Drosophila homologs of two mammalian intracellular Ca(2+)-release channels: identification and expression patterns of the inositol 1,4,5-triphosphate and the ryanodine receptor genes.
We have identified and cloned portions of two Drosophila genes homologous to two classes of mammalian intracellular Ca(2+)-release channels, the ryanodine receptor and the inositol 1,4,5-triphosphate (IP3) receptor. The Drosophila ryanodine receptor gene (dry) encodes an approx. 15 kb mRNA. It is expressed in the mesoderm of early stage-9 embryos and subsequently in somatic muscles and their precursor cells. In adults, dry mRNA was detected in tubular muscles and at a lower level in neuronal tissues. Embryonic expression of the Drosophila IP3 receptor gene (dip) appears more dynamic and is associated with developing anterior sense organs. In adults, dip expression occurs in several tissues, and relatively high levels of dip mRNA in adult antennae suggest a role for this gene product during olfactory transduction.
Hasan
G
G
Howard Hughes Medical Institute, Brandeis University, Waltham, MA 02254.
Rosbash
M
M
D000595:Amino Acid Sequence; D000818:Animals; D015220:Calcium Channels / Q000235:genetics*; D004330:Drosophila / Q000196:embryology / Q000235:genetics* / Q000648:ultrastructure; D015870:Gene Expression / Q000502:physiology*; D017403:In Situ Hybridization; D053496:Inositol 1,4,5-Trisphosphate Receptors; D008969:Molecular Sequence Data; D009124:Muscle Proteins / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011817:Rabbits; D051381:Rats; D011956:Receptors, Cell Surface / Q000235:genetics*; D011950:Receptors, Cholinergic / Q000235:genetics*; D018160:Receptors, Cytoplasmic and Nuclear; D019837:Ryanodine Receptor Calcium Release Channel; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
D015220:Calcium Channels; D053496:Inositol 1,4,5-Trisphosphate Receptors; D009124:Muscle Proteins; D011956:Receptors, Cell Surface; D011950:Receptors, Cholinergic; D018160:Receptors, Cytoplasmic and Nuclear; D019837:Ryanodine Receptor Calcium Release Channel