Distribution of cells containing progesterone receptor mRNA in the female rat di- and telencephalon: an in situ hybridization study.
In an attempt to examine regional synthesis of the progesterone receptor (PR) in the brain, the distribution of mRNA encoding PR was investigated in the female adult rat di- and telencephalon by in situ hybridization using T7 RNA polymerase transcripts of a 320 base pair rat PR cDNA clone. The rat PR cDNA had been partially cloned and sequenced by using the reverse transcription-polymerase chain reaction (RT-PCR) method. The primer set corresponds to a part of the progesterone binding domain of human PR cDNA. Large numbers of strong labeling were observed in the arcuate nucleus, medial preoptic nucleus, and ventrolateral part of the ventromedial nucleus which are relative to sexual behavior. Moderate labeling was found in layers II and IV of the isocortex, in the pyramidal layer of the CA1 and CA3 fields of the hippocampal formation, in the cortical nucleus of the amygdala, in the nucleus of the diagonal band, and in the anterior periventricular nucleus. Weak labeling was found in many other regions. These results were largely in agreement with the distribution of PR previously reported by ligand binding assay and autoradiographic studies. This present in situ hybridization study may provide a useful tool for the analysis of the regional regulation of PR synthesis in the rat brain.
Hagihara
K
K
Department of Obstetrics and Gynecology, Yamanashi Medical School, Japan.
Hirata
S
S
Osada
T
T
Hirai
M
M
Kato
J
J
D000818:Animals; D003001:Cloning, Molecular; D004027:Diencephalon / Q000166:cytology / Q000502:physiology*; D004971:Estrus; D005260:Female; D009474:Neurons / Q000166:cytology / Q000502:physiology*; D009693:Nucleic Acid Hybridization; D009928:Organ Specificity; D016133:Polymerase Chain Reaction; D015347:RNA Probes; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D011980:Receptors, Progesterone / Q000096:biosynthesis*; D013687:Telencephalon / Q000166:cytology / Q000502:physiology*
D015347:RNA Probes; D012333:RNA, Messenger; D011980:Receptors, Progesterone