Excision of a transposable element from a viral vector introduced into maize plants by agroinfection.
The geminivirus maize streak virus (MSV) was used as a vector to introduce the maize transposable element Dissociation (Ds) and to study its excision in maize plants. MSV carrying Ds1 in its genome was introduced into maize plants by agroinfection. Excision of the Ds1 element from the MSV genome was detected only when functions from the transposable element Activator (Ac) were supplied in trans, either endogenously by the recipient maize plant or by co-transformation with Agrobacterium carrying a genomic Ac clone. The excision of Ds1 could easily be visualized by the appearance of viral symptoms induced by the revertant virus. The junction sequences left on the MSV genome after excision revealed 'footprints' typical of transposition as described for maize. From these results, we conclude that transposition functions in our system and that the use of the MSV replicon provides a rapid and simple tool for the investigation of the excision of transposable elements in maize plants.
Shen
W H
WH
Friedrich Miescher-Institut, Basel, Switzerland.
Hohn
B
B
D016960:Agrobacterium tumefaciens / Q000235:genetics; D001483:Base Sequence; D004247:DNA / Q000235:genetics; D004251:DNA Transposable Elements; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D010942:Plant Viruses / Q000235:genetics / Q000472:pathogenicity; D010944:Plants / Q000235:genetics* / Q000382:microbiology; D010957:Plasmids; D016133:Polymerase Chain Reaction; D003313:Zea mays / Q000235:genetics / Q000382:microbiology
D004251:DNA Transposable Elements; D004247:DNA