Supports for enzyme immobilization.
Macroporous silica gel, prepared from sodium silicate and hydrochloric acid, one grade of diatomaceous earth (celite 545) and nonporous glass were analyzed in terms of pore size by the mercury intrusion pressure method. The alkylamine derivatives of the above materials were examined for their suitability as supports for enzyme immobilization, using the enzyme glucose oxidase. The effectiveness of the immobilized enzyme was compared in relation to the free enzyme and particle size of the carrier. The immobilized enzyme exhibited a broader range of optimum pH and greater thermal stability, among some properties considered.
Trevisan
H C
HC
Faculdade de Engenharia Química, Departamento de Ciência e Tecnologia de Materiais, Universidade Estadual de Campinas, SP, Brazil.
Mei
L H
LH
D004800:Enzymes, Immobilized / Q000378:metabolism*; D005782:Gels; D005949:Glucose Oxidase / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008628:Mercury; D016062:Porosity; D012822:Silicon Dioxide; D013696:Temperature; D013997:Time Factors
D004800:Enzymes, Immobilized; D005782:Gels; D012822:Silicon Dioxide; D005949:Glucose Oxidase; D008628:Mercury