Immunocytochemical detection of herpes viruses in oral smears of HIV-infected patients.
Cytologic smears (CS) were taken from the lateral border of the tongue of HIV-seropositive patients (HIV+) (n = 39) and of seronegative controls (HIV-) (n = 19) and examined by immunocytochemistry (APAAP) and in situ hybridization (ISH) (biotinylated DNA probes) for the presence of viral antigens/DNA of EBV and CMV. While none of the HIV controls showed positive results for EBV antigen, 61% (APAAP) resp. 79% (ISH) of oral epithelial cells in the group of HIV+ patients were EBV-positive. While all CS taken from areas with the clinical diagnosis of hairy leukoplakia (HL) were EBV positive (APAAP and/or ISH), the detection of EBV in CS from uninvolved oral mucosa seemed to be associated with the later development of HL. In the group of HIV+ patients the detection rate for CMV was about five times (APAAP) resp. three times (ISH) higher than in HIV- persons. This non-invasive technique seems to be a valuable tool to screen for viral antigens/genomes.
Langford
A
A
Abteilung für zahnärztliche Chirurgiel Oralchirurgie-Nord, Freie Universität, Berlin.
Kunze
R
R
Schmelzer
S
S
Wolf
H
H
Pohle
H D
HD
Reichart
P
P
D000328:Adult; D002467:Cell Nucleus / Q000382:microbiology; D003581:Cytodiagnosis; D003587:Cytomegalovirus / Q000235:genetics / Q000302:isolation & purification*; D003593:Cytoplasm / Q000382:microbiology; D015342:DNA Probes; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D015658:HIV Infections / Q000382:microbiology* / Q000473:pathology; D006679:HIV Seropositivity / Q000382:microbiology / Q000473:pathology; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D007150:Immunohistochemistry; D015603:Keratinocytes / Q000382:microbiology; D007972:Leukoplakia, Oral / Q000382:microbiology; D008297:Male; D009693:Nucleic Acid Hybridization; D014059:Tongue / Q000382:microbiology* / Q000648:ultrastructure; D014062:Tongue Neoplasms / Q000382:microbiology; D014412:Tumor Virus Infections / Q000382:microbiology; D014764:Viral Proteins / Q000032:analysis
D015342:DNA Probes; D004279:DNA, Viral; D014764:Viral Proteins