Marek's disease virus-transformed chicken T-cell lines respond to lymphokines.
Current assays for chicken interleukin-2 (IL-2) utilize mitogen-activated lymphocytes. However, very high inter-assay variability and sporadic high background proliferation limit their usefulness. In view of the above, several Marek's disease virus (MDV)-transformed T-cell lines (which grow well in a serum-supplemented medium) were tested for a response to chicken IL-2 when grown in serum-free media. Five of six lines examined showed a dose-dependent proliferative response to chicken T-cell conditioned media. One line, MDCC-CU14, was chosen for further studies. In addition to the tumor cells' dose-dependent responses to semi-purified chicken IL-2, they expressed T-cell activation antigens on the cell surface. Furthermore, the level of surface expression was enhanced on cells provided IL-2. Co-incubation of the tumor cells with monoclonal antibody INN-CH-16 (specific for an antigen on the surface of activated T-cells) and IL-2 resulted in a modulation of lymphokine-induced proliferation. Together, these data suggest that signalling mechanisms in MDV T-cell tumors are intact and that these lines can be used as an assay for chicken T-cell lymphokines. Furthermore, they provide an interesting model for the study of avian and mammalian T-cell transformation. Implications for the study of Marek's disease are also discussed.
Kaplan
M H
MH
Department of Immunology and Microbiology, Wayne State University School of Medicine, Detroit, MI 48201.
Dhar
A
A
Brown
T R
TR
Sundick
R S
RS
D000818:Animals; D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral / Q000276:immunology*; D002645:Chickens / Q000276:immunology*; D017077:Culture Media, Conditioned / Q000494:pharmacology; D005434:Flow Cytometry; D008381:Herpesvirus 2, Gallid / Q000502:physiology*; D007376:Interleukin-2 / Q000502:physiology; D008213:Lymphocyte Activation / Q000276:immunology; D008380:Marek Disease / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7
D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D017077:Culture Media, Conditioned; D007376:Interleukin-2; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7