Molecular cloning of a new bombesin receptor subtype expressed in uterus during pregnancy.
The homology screening approach has been used to clone a new member of the guanine-nucleotide-binding-protein-coupled receptor superfamily from guinea pig uterus. The cloned cDNA encodes a 399-amino-acid protein and shows the highest amino acid similarity to members of the bombesin receptor family; 52% and 47% similarity to the gastrin-releasing-peptide (GRP) receptor and the neuromedin-B receptor, respectively. Binding experiments with the stably transfected LLC-PK1 cell line expressing the new receptor protein confirmed the bombesin-like nature of the cloned receptor. The relative order of ligand affinity, GRP = neuromedin C much greater than neuromedin B, suggests that the cloned cDNA represents the GRP subtype rather than the neuromedin-B subtype of bombesin receptors. Northern-blot analysis of mRNA species from several guinea-pig tissues showed that the mRNA for the new bombesin receptor subtype is expressed mainly in uteri of pregnant animals.
Gorbulev
V
V
Max-Planck-Institut für Biophysik, Frankfurt, Federal Republic of Germany.
Akhundova
A
A
Büchner
H
H
Fahrenholz
F
F
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D004247:DNA / Q000737:chemistry / Q000302:isolation & purification; D005260:Female; D015870:Gene Expression; D006168:Guinea Pigs; D007668:Kidney; D008969:Molecular Sequence Data; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D018004:Receptors, Bombesin; D017981:Receptors, Neurotransmitter / Q000737:chemistry / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D014162:Transfection; D014599:Uterus / Q000378:metabolism*
D012333:RNA, Messenger; D018004:Receptors, Bombesin; D017981:Receptors, Neurotransmitter; D004247:DNA