The protein kinase inhibitor, staurosporine, does not inhibit phosphoinositide kinases.
The effects of the protein kinase inhibitor staurosporine on phosphatidylinositol (PI) kinase and phosphatidylinositol 4-phosphate (PIP) kinase activities in brain membranes, and the synthesis of polyphosphoinositides in cultured NIH 3T3 fibroblasts were examined. Staurosporine, at concentrations up to 1 microM did not significantly modulate either PI kinase or PIP kinase activity in brain membranes. DNA synthesis was dose-dependently inhibited by sub-nanomolar concentrations of staurosporine; however, cells treated with up to 1 microM staurosporine contained levels of PIP and phosphatidylinositol 4,5-bisphosphate (PIP2) similar to those observed in untreated cells. Therefore, phosphoinositide kinases do not appear to be either directly or indirectly affected by staurosporine.
Smith
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Cancer Research Center of Hawaii, University of Hawaii, Honolulu 96813.
Mooberry
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D019870:1-Phosphatidylinositol 4-Kinase; D016475:3T3 Cells / Q000187:drug effects / Q000378:metabolism; D000470:Alkaloids / Q000494:pharmacology*; D000818:Animals; D001921:Brain / Q000201:enzymology; D004247:DNA / Q000096:biosynthesis; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D010716:Phosphatidylinositols / Q000378:metabolism; D010770:Phosphotransferases / Q000037:antagonists & inhibitors*; D047428:Protein Kinase Inhibitors; D051381:Rats; D019311:Staurosporine
D000470:Alkaloids; D010716:Phosphatidylinositols; D047428:Protein Kinase Inhibitors; D004247:DNA; D010770:Phosphotransferases; D019870:1-Phosphatidylinositol 4-Kinase; D019311:Staurosporine