Modulation of the human papillomavirus type 16 induced transformation and transcription by deletion of loci on the short arm of human chromosome 11 can be mimicked by SV40 small t.
The human papillomavirus (HPV) type 16 enhancer-promoter has been shown to be active in human fibroblasts with a deletion on the short arm of one chromosome 11 (karyotype 46,del(11)(p11.11p15.1)) but is virtually inactive in diploid human fibroblasts (Smits, Smits, Jebbink, and ter Schegget, 1990b, Virology, 176, 158-165). In diploid human embryonic fibroblasts, activation of the HPV16 enhancer-promoter could be achieved by expression of the SV40 small t. By cotransfecting SV40 small t cDNA together with HPV16 DNA into diploid cells, it was possible to increase the transforming activity of HPV16 by 10- 15-fold. Furthermore, SV40 small t was essential for the SV40 large T-induced morphological transformation of human diploid fibroblasts, whereas SV40 small t was dispensable for transformation of del-11 cells. We propose that, as a result of the deletion of loci on the short arm of chromosome 11 in del-11 cells, functions are expressed that mimic those of SV40 small t in transformation and trans-activation.
Smits
P H
PH
Department of Medical Microbiology, University of Amsterdam, The Netherlands.
de Ronde
A
A
Smits
H L
HL
Minnaar
R P
RP
van der Noordaa
J
J
ter Schegget
J
J
D000952:Antigens, Polyomavirus Transforming / Q000378:metabolism*; D002460:Cell Line; D002472:Cell Transformation, Viral; D002872:Chromosome Deletion; D002880:Chromosomes, Human, Pair 11; D004171:Diploidy; D004742:Enhancer Elements, Genetic; D006801:Humans; D027383:Papillomaviridae / Q000235:genetics*; D011401:Promoter Regions, Genetic; D015534:Trans-Activators / Q000378:metabolism*; D014158:Transcription, Genetic
D000952:Antigens, Polyomavirus Transforming; D015534:Trans-Activators