Quantitative autoradiography of hemicholinium-3 binding sites in human amygdala.
We report the binding properties and subnuclear localization of [3H]hemicholinium-3 binding sites in human amygdala using quantitative autoradiography. Specific binding was saturable and high affinity (apparent Kd 2-11 nM). Binding was highest in the basolateral nucleus which receives dense cholinergic innervation from the basal forebrain. Binding closely approximated acetylcholinesterase reactivity. These data support [3H]hemicholinium-3 as a quantitative marker for cholinergic terminals in human brain.
Wolf
S S
SS
Department of Neurology, University of Virginia Health Sciences Center, Charlottesville 22908.
Brashear
H R
HR
Levesque
C A
CA
Wooten
G F
GF
D000368:Aged; D000369:Aged, 80 and over; D000679:Amygdala / Q000378:metabolism*; D001345:Autoradiography; D002352:Carrier Proteins; D005260:Female; D006426:Hemicholinium 3 / Q000378:metabolism*; D006801:Humans; D008297:Male; D008875:Middle Aged; D011869:Radioligand Assay; D011956:Receptors, Cell Surface / Q000032:analysis*; D011950:Receptors, Cholinergic / Q000032:analysis*
D002352:Carrier Proteins; D011956:Receptors, Cell Surface; D011950:Receptors, Cholinergic; D006426:Hemicholinium 3