Detection of cytomegalovirus DNA in pulmonary specimens: confirmation by in situ hybridization in two cases.
Papanicolaou stained bronchial brush and imprint pulmonary smears containing intranuclear and cytoplasmic inclusion bearing alveolar pneumocytes suggestive of cytomegalovirus infection were destained and reprocessed for in situ hybridization using a biotinylated probe for cytomegalovirus DNA. Two cases were processed in this way. A hybridization signal for viral DNA was noted in each case. However, no reddish brown staining reaction products were noted in any of the control samples. This simple and rapid nonradioactive detection system is a valuable supplement to routine pulmonary cytology for the definitive diagnosis of this virus infection, and this technique is also appropriate for retrospective study.
Iwa
N
N
Department of Pathology, Osaka City University Hospital, Japan.
Sasaki
M
M
Yutani
C
C
Wakasa
K
K
D003581:Cytodiagnosis / Q000379:methods; D003587:Cytomegalovirus / Q000235:genetics*; D003586:Cytomegalovirus Infections / Q000175:diagnosis*; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D006801:Humans; D008171:Lung Diseases / Q000382:microbiology* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009693:Nucleic Acid Hybridization
D004279:DNA, Viral