Effect of dimethyl sulfoxide on cytosolic calcium in cultured rat hepatocytes injured by D-galactosamine.
D-galactosamine (Gal 0.5 mmol.L-1) made lactate dehydrogenase (LDH) leakage from the hepatocytes in monolayer-culture increase by 50%, Dimethyl sulfoxide (Me2SO 2% vol/vol) decreased the LDH leakage (P less than 0.05). The cytosolic free Ca2+ concentration ([Ca2+]c) of rat hepatocytes exposed to Gal 4 mmol.L-1 for 90 min in suspension culture increased about 2-fold (P less than 0.01). Me2SO (2%) antagonized this [Ca2+]c-increasing effect of Gal. These results verified directly that the [Ca2+]c of hepatocytes was increased in the early stage of Gal-induced hepatotoxicity, and suggested that the prevention or lightening of the disturbance in intracellular Ca2+ homeostasis may be, at least, one of the mechanisms of the protective action of Me2SO against Gal-induced hepatocyte injury.
Liu
H P
HP
Department of Pharmacology, Beijing Medical University, China.
Cong
Z
Z
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D000252:Calcium-Transporting ATPases / Q000502:physiology; D002478:Cells, Cultured; D003600:Cytosol / Q000378:metabolism; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D005688:Galactosamine / Q000494:pharmacology; D008099:Liver / Q000166:cytology* / Q000187:drug effects; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
D005688:Galactosamine; D000252:Calcium-Transporting ATPases; D002118:Calcium; D004121:Dimethyl Sulfoxide