Genetically defined lysosomal acetylesterase EC 3.1.1.6 in the cauda epididymidis of mouse, rat, and man.
After inhibition by bis-p-nitrophenyl phosphate and subsequent staining for esterase using naphthol AS-D acetate as the substrate, a strong lysosomal esterase was demonstrated in the cauda epididymidis of mouse, rat, and man. Owing to its behaviour towards the classifying inhibitors eserine, diisopropyl fluorophosphate, bis-p-nitrophenyl phosphate, and p-chloromercuriphenylsulphonate, this lysosomal esterase was shown to be an acetylesterase (EC 3.1.1.6). Control experiments involving isoelectric focusing revealed that this acetylesterase was identical with the genetically defined homologues ES-17, ES-6, and ES-A4 in mouse, rat, and man, respectively.
Trefzger
J
J
Abteilung für Chemische Pathologie, Universität Freiburg, Germany.
Ronai
A
A
Wassmer
B
B
von Deimling
O
O
D002731:4-Chloromercuribenzenesulfonate / Q000494:pharmacology; D000115:Acetylesterase / Q000032:analysis* / Q000037:antagonists & inhibitors; D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D004822:Epididymis / Q000201:enzymology*; D006651:Histocytochemistry; D006801:Humans; D007150:Immunohistochemistry; D007525:Isoelectric Focusing; D007531:Isoflurophate / Q000494:pharmacology; D008247:Lysosomes / Q000201:enzymology*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009596:Nitrophenols / Q000494:pharmacology; D010830:Physostigmine / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains
D009596:Nitrophenols; D007531:Isoflurophate; D002731:4-Chloromercuribenzenesulfonate; C002887:bis(4-nitrophenyl)phosphate; D010830:Physostigmine; D000115:Acetylesterase