[The use of nucleolytic enzymes (ribonucleases, polynucleotide phosphorylases and endonuclease from Serratia marcescens) for producing initial blocks of synthetic endoribonucleases].
The simplest variant of synthetic substrate-ribozyme complex has been proposed. The schemes of potential ribozyme "subunits" synthesis have been worked out: R1--GCUUGAAACAAA; R2--AAAAACUGAUGAAAGC. The macroscale synthesis of dinucleoside monophosphate ApU, GpC, CpU catalyzed by immobilized ribonucleases of different specificity and preparation of oligoadenylates by hydrolysis of poly-A in the presence of endonuclease Serratia marcescens, as well the synthesis of conservative sequences of potential ribozyme such as ApUpG, CpUpG, GpApU, ApApApG and others have been described.
Kliagina
V P
VP
Sedel'nikova
E A
EA
Smolianinova
O A
OA
Soboleva
I A
IA
Khabarova
M I
MI
Zhenodarova
S M
SM
D001483:Base Sequence; D004720:Endonucleases / Q000378:metabolism*; D004722:Endoribonucleases / Q000138:chemical synthesis*; D004800:Enzymes, Immobilized / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology; D008969:Molecular Sequence Data; D011117:Polyribonucleotide Nucleotidyltransferase / Q000378:metabolism*; D016337:RNA, Catalytic / Q000138:chemical synthesis; D012260:Ribonucleases / Q000378:metabolism*; D012706:Serratia marcescens / Q000201:enzymology*; D013379:Substrate Specificity
D004800:Enzymes, Immobilized; D016337:RNA, Catalytic; D011117:Polyribonucleotide Nucleotidyltransferase; D004720:Endonucleases; D004722:Endoribonucleases; D012260:Ribonucleases
Primenenie nukleoliticheskikh fermentov (ribonukleaz, polinukleotidfosforilaz, éndonukleazy Serratia marcescens) dlia polucheniia iskhodnykh blokov sinteticheskikh éndoribonukleaz.