Presence of a Vicia unijuga lectin-binding (Vgu) glycoprotein with Thomsen-Friedenreich (T) activity and Vgu glycoproteins in human primary hepatocellular carcinoma.
1. Twenty perchloric acid-soluble glycoprotein fractions (PASFs) were separated from carcinomatous and non-carcinomatous regions of the livers of 5 patients with primary hepatocellular carcinoma (HCC) and 5 patients with a complication of HCC and hepatocirrhosis (HC). 2. In autoradiography using 125I-labelled Vicia unijuga lectin (VUA) and 125I-labelled Arachis hypogaea anti-T lectin as probes, 10 PASFs from carcinomatous regions of the livers of 10 patients gave 1 Vgu glycoprotein with T activity and/or 1-4 Vgu glycoproteins, respectively, and among 10 PASFs from non-carcinomatous regions of the livers of 10 patients, 7 PASFs gave no glycoproteins, respectively. 3. These results show that a Vgu glycoprotein with T activity and Vgu glycoproteins occur in HCC and in a complication of HCC and HC as oncofetal antigens.
Otsuka
K
K
Department of Biochemistry, Tokyo College of Pharmacy, Japan.
Mizuno
M
M
Kagawa
K
K
Kashima
K
K
Ohkuma
S
S
D000906:Antibodies; D015295:Antigens, Tumor-Associated, Carbohydrate; D006528:Carcinoma, Hepatocellular / Q000737:chemistry*; D004187:Disaccharides / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins / Q000032:analysis* / Q000378:metabolism; D006801:Humans; D037102:Lectins / Q000378:metabolism*; D008113:Liver Neoplasms / Q000737:chemistry*; D009363:Neoplasm Proteins / Q000032:analysis* / Q000378:metabolism; D010472:Perchlorates / Q000494:pharmacology; D037121:Plant Lectins; D012995:Solubility
D000906:Antibodies; D015295:Antigens, Tumor-Associated, Carbohydrate; D004187:Disaccharides; D006023:Glycoproteins; D037102:Lectins; D009363:Neoplasm Proteins; D010472:Perchlorates; D037121:Plant Lectins; C042242:Vicia lectins; C016467:Thomsen-Friedenreich antigen; C025991:Thomsen-Friedenreich antibodies