Effect of beta-lactamase inhibitors on beta-lactamases from anaerobic bacteria.
Three beta-lactamase inhibitors in clinical use--clavulanic acid, sulbactam and tazobactam--were investigated for their activity on beta-lactamases from Bacteroides uniformis, Clostridium butyricum and Fusobacterium nucleatum. Purification of the beta-lactamases was carried out by anion-exchange chromatography, gel filtration and FPLC. The inactivation of beta-lactamase activity was determined spectrophotometrically with nitrocefin as substrate. Various concentrations of the inhibitors were preincubated at 30 degrees C together with the enzyme for different periods of time before determination of the beta-lactamase activity. The three beta-lactamases tested were more susceptible to tazobactam than to clavulanic acid and sulbactam. Clavulanic acid and sulbactam reduced the enzyme activity of the Bacteroides uniformis beta-lactamase more effectively than the Clostridium butyricum and Fusobacterium nucleatum beta-lactamases.
Hedberg
M
M
Department of Microbiology, Karolinska Institute, Huddinge University Hospital, Sweden.
Lindqvist
L
L
Tunér
K
K
Nord
C E
CE
D001421:Bacteria, Anaerobic / Q000187:drug effects / Q000201:enzymology*; D001439:Bacteroides / Q000187:drug effects / Q000201:enzymology; D019818:Clavulanic Acid; D002969:Clavulanic Acids / Q000494:pharmacology*; D003013:Clostridium / Q000187:drug effects / Q000201:enzymology; D016967:Fusobacterium nucleatum / Q000187:drug effects / Q000201:enzymology; D010397:Penicillanic Acid / Q000494:pharmacology*; D013407:Sulbactam / Q000494:pharmacology*; D000078142:Tazobactam; D065093:beta-Lactamase Inhibitors
D002969:Clavulanic Acids; D065093:beta-Lactamase Inhibitors; D019818:Clavulanic Acid; D010397:Penicillanic Acid; D013407:Sulbactam; D000078142:Tazobactam