title: Long-term depression is induced in Ca2+/calmodulin kinase-inhibited visual cortex neurons.
abstract: To elucidate a role of Ca2+/calmodulin-dependent protein kinase II (CaMKII) in induction of long-term potentiation (LTP), KN-62, a selective inhibitor for CaMKII, was injected into layer 2/3 neurons of sliced visual cortex obtained from young rats. Tetanic stimulation (5 Hz, 1 min) applied to the white matter after the KN-62 injection induced long-term depression (LTD) of excitatory postsynaptic potentials (EPSPs) evoked by test stimulation of the white matter in 9 of the 14 cells tested. However, EPSPs evoked by test stimulation of the non-tetanized site were not changed, indicating that the induction of LTD was input-specific. Simultaneously, recorded field potentials which were derived from neurons with intact CaMKII showed LTP. These results suggest that postsynaptic CaMKII plays a role in the induction of LTP/LTD in visual cortex.
authors.affiliation: Department of Neurophysiology, Biomedical Research Center, Osaka University Medical School, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Funauchi
authors.forename: T
authors.initials: T
authors.lastname: Tsumoto
authors.forename: A
authors.initials: A
authors.lastname: Nishigori
authors.forename: Y
authors.initials: Y
authors.lastname: Yoshimura
authors.forename: H
authors.initials: H
authors.lastname: Hidaka
mesh_terms: D000818:Animals; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D005074:Evoked Potentials, Visual / Q000502:physiology*; D066298:In Vitro Techniques; D009474:Neurons; D047428:Protein Kinase Inhibitors; D051381:Rats; D011919:Rats, Inbred Strains; D013569:Synapses / Q000502:physiology*; D013997:Time Factors; D014793:Visual Cortex / Q000166:cytology*
chemical_list: D047428:Protein Kinase Inhibitors; D017871:Calcium-Calmodulin-Dependent Protein Kinases