title: Sequential assignment of the proton NMR spectrum of isolated alpha(CO) chains from human adult hemoglobin.
abstract: In this paper we report proton two-dimensional NMR experiments on isolated alpha chains from human hemoglobin A (HbA) in the monocarboxylated state. Several J-correlated and NOE spectra in water or deuterium water and phosphate buffer (100 mM) at 310 K and pH 5.6 were acquired and analysed for the sequential assignment of the proton resonances. In addition, we used the topological data obtained from the crystal structure of alpha subunits in the monocarboxylated HbA tetramer. The assigned resonances correspond to 70% of the amino acid residues. The present results provide information on the tertiary structure of isolated alpha chains in solution, particularly in the heme region. This structure may be compared with that of the a subunits in the tetrameric HbA(CO) in crystal by comparison of observed chemical shifts and those calculated from the X-ray atomic coordinates. Overall, the global folding of the two forms are highly similar. However, this analysis points out several local conformational differences in the heme pocket and the neighboring of the unique Trp residue. Possible explanations of these differences are discussed.
authors.affiliation: Institut National de la Santé et de la Recherche Médicale U91, Hôpital Henri Mondor, Créteil, France.
authors.forename: L
authors.initials: L
authors.lastname: Martineau
authors.forename: C T
authors.initials: CT
authors.lastname: Craescu
mesh_terms: D000595:Amino Acid Sequence; D002263:Carboxyhemoglobin / Q000737:chemistry*; D006441:Hemoglobin A / Q000737:chemistry*; D006801:Humans; D046911:Macromolecular Substances; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D011487:Protein Conformation; D011522:Protons
chemical_list: D046911:Macromolecular Substances; D011522:Protons; D006441:Hemoglobin A; D002263:Carboxyhemoglobin