Complementation of the cs dis2-11 cell cycle mutant of Schizosaccharomyces pombe by a protein phosphatase from Arabidopsis thaliana.
The activities of type I protein phosphatases play a central role in eukaryotic cell cycle control. Here, we report the cloning and characterization from the flowering plant Arabidopsis thaliana of a cDNA clone named PP1-At which is highly homologous to protein phosphatase 1. The deduced amino acid sequence of PP1-At shows that the PP1-At protein is 318 amino acid residues long and has a molecular weight of 35,298 Da. The PP1-At protein has strong similarity to all other known protein phosphatase type 1 catalytic subunits. Approximately 62% of the amino acids are identical to type 1 protein phosphatases of rabbit, mouse, Saccharomyces cerevisiae and Schizosaccharomyces pombe. RNA blot analysis revealed a single mRNA species of approximately the same size as the cDNA isolated. The PP1-At-encoded mRNA of 1.3 kb is abundant in most vegetative Arabidopsis tissues, with the lowest level of expression in leaves. When transferred to the fission yeast S.pombe, the PP1-At-encoded protein can rescue a semidominant mutant, cold sensitive (cs) dis2-11, which under nonpermissive conditions is unable to complete chromosome disjunction.
Nitschke
K
K
Max-Planck-Institut für Züchtungsforschung, Köln, FRG.
Fleig
U
U
Schell
J
J
Palme
K
K
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002453:Cell Cycle / Q000235:genetics*; D003001:Cloning, Molecular; D003080:Cold Temperature; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D005799:Genes, Dominant; D017343:Genes, Plant; D005816:Genetic Complementation Test; D051379:Mice; D008969:Molecular Sequence Data; D009154:Mutation; D009838:Oligodeoxyribonucleotides; D010749:Phosphoprotein Phosphatases / Q000235:genetics* / Q000378:metabolism; D010944:Plants / Q000201:enzymology* / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods; D011487:Protein Conformation; D054645:Protein Phosphatase 1; D011817:Rabbits; D015183:Restriction Mapping; D012568:Schizosaccharomyces / Q000166:cytology / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
D009838:Oligodeoxyribonucleotides; D004247:DNA; D010749:Phosphoprotein Phosphatases; D054645:Protein Phosphatase 1