Herpes simplex virus type 1 and human immunodeficiency virus type 1 antigens in platelets from a hemophilia B patient with human immunodeficiency virus type 1-related thrombocytopenia.
We analyzed platelet-associated antigens from a hemophilia B patient with human immunodeficiency virus type 1 (HIV-1)-related thrombocytopenia. Two bands appeared at 31,000 and 37,000 daltons in the platelet lysate after reaction with autologous serum in SDS-PAGE and Western blots. The band at 37,000 daltons was obtained using anti-herpes simplex type 1 (HSV-1) rabbit antiserum. Doublet bands at 36,000 and 37,000 daltons also appeared after reaction with HSV-1 seropositive human serum. The band at 31,000 daltons appeared after reaction with anti-HIV-1 rabbit serum. These results suggest that the platelet-associated antigens in this patient are components of both HSV-1 and HIV-1 antigens. In addition, acyclovir decreased his PAIgG level and increased his platelet count, and zidovudine increased his platelet count. Thus, we concluded that each of the platelet-associated antigens is partially responsible for the thrombocytopenia by causing deposition of immune complexes in this patient.
Koike
K
K
Department of Clinical Pathology, Tokyo Medical College, Japan.
Matsumoto
K
K
Arai
M
M
Yorifuji
H
H
Fukutake
K
K
Fujimaki
M
M
D000212:Acyclovir / Q000627:therapeutic use; D000328:Adult; D016824:Antigens, Human Platelet / Q000276:immunology; D000956:Antigens, Viral / Q000032:analysis*; D001792:Blood Platelets / Q000276:immunology / Q000382:microbiology*; D015488:HIV Antigens / Q000032:analysis*; D015658:HIV Infections / Q000150:complications* / Q000188:drug therapy / Q000382:microbiology; D015497:HIV-1 / Q000276:immunology / Q000302:isolation & purification*; D006467:Hemophilia A / Q000150:complications* / Q000382:microbiology; D006561:Herpes Simplex / Q000150:complications* / Q000382:microbiology; D006801:Humans; D007105:Immune Complex Diseases / Q000209:etiology; D007074:Immunoglobulin G / Q000032:analysis; D008297:Male; D008970:Molecular Weight; D018139:Simplexvirus / Q000276:immunology / Q000302:isolation & purification*; D013921:Thrombocytopenia / Q000150:complications* / Q000382:microbiology; D015215:Zidovudine / Q000627:therapeutic use
D016824:Antigens, Human Platelet; D000956:Antigens, Viral; D015488:HIV Antigens; D007074:Immunoglobulin G; D015215:Zidovudine; D000212:Acyclovir