title: Comparison between first- and second-generation test for anti-hepatitis C virus antibodies in hemodialysis patients.
abstract: The serum of 387 hemodialysis patients from 9 dialysis units was checked for anti-hepatitis C virus antibodies with a 1st-generation ELISA (Ortho) test: 61 patients were repeatedly positive. In order to avoid false-positive results, these sera were tested with a 1st-generation confirmatory RIBA test, 2nd-generation screening ELISA test and 2nd-generation confirmatory RIBA test. The 2nd-generation ELISA test confirmed data obtained with 1st-generation ELISA, however, the 1st-generation confirmatory RIBA test underestimated the number of anti-HCV-positive patients.
authors.affiliation: Department of Nephrology, General Hospital, Treviso, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Da Porto
authors.forename: P
authors.initials: P
authors.lastname: Poli
authors.forename: P
authors.initials: P
authors.lastname: Calzavara
authors.forename: M R
authors.initials: MR
authors.lastname: Castelletto
authors.forename: A
authors.initials: A
authors.lastname: Adami
authors.forename: C
authors.initials: C
authors.lastname: Cascone
authors.forename: G P
authors.initials: GP
authors.lastname: Amici
authors.forename: T
authors.initials: T
authors.lastname: Teodori
authors.forename: L
authors.initials: L
authors.lastname: Okolicsanyi
mesh_terms: D003951:Diagnostic Errors; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D016174:Hepacivirus / Q000276:immunology*; D006508:Hepatitis Antibodies / Q000097:blood*; D006526:Hepatitis C / Q000175:diagnosis / Q000276:immunology; D006801:Humans; D015151:Immunoblotting / Q000379:methods; D006435:Renal Dialysis / Q000009:adverse effects*
chemical_list: D006508:Hepatitis Antibodies