[Terminal deoxynucleotidyl transferase in various immunophenotypic cells in leukemia].
The staining results of terminal deoxynucleotidyl transferase (TdT) in leukemic cells of peripheral blood or bone marrow in 55 patients showed that 22 (40%) patients were found positive for TdT staining: 17 patients with acute lymphoblastic leukemia (ALL), 2 acute non-lymphocytic leukemia (ANLL) and 3 hybrid acute leukemia (HAL). The positive rate of ALL (17/21) was significantly higher than that of ANLL (2/21, P < 0.005). The leukemic cells of HLA-DR+CD19+TdT- were demonstrated to express some of myeloid restricted antigens (HI98, HIM 4, HIM 5) (3/3), which suggested that these cases were leukemias of myeloid origin. None of the 8 patients with M 0/M 1 ANLL was positively stained for the enzyme. Our results showed that TdT detection is of great value in the diagnosis and differentiation of both poorly differentiated and hybrid acute leukemia.
Tang
Y M
YM
Children's Hospital, Zhejiang Medical University, Hangzhou.
D000328:Adult; D000911:Antibodies, Monoclonal; D002648:Child; D004253:DNA Nucleotidylexotransferase / Q000032:analysis*; D003937:Diagnosis, Differential; D006801:Humans; D016130:Immunophenotyping; D015470:Leukemia, Myeloid, Acute / Q000175:diagnosis / Q000201:enzymology / Q000473:pathology*; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000175:diagnosis / Q000201:enzymology / Q000473:pathology*
D000911:Antibodies, Monoclonal; D004253:DNA Nucleotidylexotransferase