title: [Transposition of Tn917 in Bacillus pumilus].
abstract: Transposition Tn917 was introduced into Bacillus pumilus 289 by protoplast transformation with plasmid pTV32. The temperature-sensitive replication property of pTV32 was maintained in B. pumilus. Tn917 was transposed efficiently in B. pumilus with 4.8 x 10(-4) transposition rate. The yield of auxotrophs was about 0.65% in all insertional mutants. It indicated a prospects for the use of Tn917 as a tool for insertional mutagenesis and genetic manipulation in B. pumilus.
authors.affiliation: Department of Biology, Nankai University, Tianjin.
authors.forename: Y
authors.initials: Y
authors.lastname: Geng
authors.forename: R
authors.initials: R
authors.lastname: Jiang
mesh_terms: D001407:Bacillus / Q000235:genetics*; D004251:DNA Transposable Elements; D010957:Plasmids
chemical_list: D004251:DNA Transposable Elements