[Proteins induced by various types of double-stranded RNA and interferon type I in human fibroblasts. Correlation with antiviral activity of the substances].
Protein induction by new antiviral preparations of dsRNAs (larifan, ridostin, rifastin and poly(A).poly(U)) and recombinant beta-interferon in human fibroblasts (M19) was studied. The common gene products: 88, 80, 68, 58, 56, 52, 50 and 26 kD were detected in the spectra of the induced cytoplasmic polypeptides. At the same time the sets of the induced proteins had individual distinctions in various preparations. Induction of the 56-kD protein was more essential in the action of dsRNAs than that of interferon. The antiviral activity of dsRNAs and interferon preparations correlated with a relative increase in the synthesis of proteins with molecular weights of 88, 80 and 58 kD. The study results are in agreement with the fact that the dsRNAs have interferon-independent pathways of antiviral action with participation of 56- and 58-kD protein genes.
Sokolova
T M
TM
Antonov
A Iu
AIu
Suetina
I A
IA
D000998:Antiviral Agents; D003470:Culture Media; D003598:Cytoskeletal Proteins / Q000032:analysis / Q000096:biosynthesis*; D004666:Encephalitis Virus, Venezuelan Equine / Q000187:drug effects* / Q000502:physiology; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D006131:Growth Inhibitors; D006801:Humans; D066298:In Vitro Techniques; D016899:Interferon-beta / Q000494:pharmacology*; D008954:Models, Biological; D008970:Molecular Weight; D012330:RNA, Double-Stranded / Q000494:pharmacology*; D014721:Vesicular stomatitis Indiana virus / Q000187:drug effects* / Q000502:physiology; D014779:Virus Replication / Q000187:drug effects / Q000502:physiology
D000998:Antiviral Agents; D003470:Culture Media; D003598:Cytoskeletal Proteins; D006131:Growth Inhibitors; D012330:RNA, Double-Stranded; D016899:Interferon-beta
Belki, indutsirovannye raznymi vidami dvispiral'nykh RNK i interferonami tipa I v fibroblastakh cheloveka. Korreliatsiia s antivirusnoĭ aktivnost'iu preparatov.