Immune responses and protection induced in mice by an industrialized vaccine against American cutaneous leishmaniasis.
An industrialized vaccine against American cutaneous leishmaniasis was compared to a laboratory made vaccine in its ability to induce cellular and humoral immune responses in mice. No differences were observed between seric IgG levels or lymphoblastic proliferation response of mice immunized with either vaccine. Antigenic composition, evaluated by SDS-PAGE, was identical in both preparations. Protection induced in mice against a challenge with infective parasites was also compared. The level of protection obtained with the industrialized vaccine was comparable to that induced by the laboratory made preparation. The results showed that the industrialization process did not alter the efficacy of the vaccine.
da Costa
C A
CA
Departamento de Analises Clinicas e Toxicologicas, Faculdade de Farmacia, UFMG, Belo Horizonte, MG, Brazil.
Afonso
L C
LC
Toledo
V P
VP
Guimaraes
T M
TM
Nascimento
E
E
Tavares
C A
CA
Mayrink
W
W
D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D005069:Evaluation Studies as Topic; D005260:Female; D049673:History, 20th Century; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007891:Leishmania / Q000276:immunology*; D016773:Leishmaniasis, Cutaneous / Q000276:immunology / Q000517:prevention & control*; D008213:Lymphocyte Activation; D051379:Mice; D008810:Mice, Inbred C57BL / Q000276:immunology; D016052:Protozoan Vaccines / Q000266:history / Q000276:immunology*; D014611:Vaccination
D000913:Antibodies, Protozoan; D007074:Immunoglobulin G; D016052:Protozoan Vaccines