title: [Genes of the lipase family: comparison of nucleic and proteinic sequences].
abstract: Vertebrates' plasmatic apolipoproteins and a few number of lipases in their metabolism present sequence homologies. They are grouped in genes families. The four exons apolipoproteins gene family includes nine human genes: the divergence rate of their sequences allows to place the first ancestral gene very high in the phylogenetic tree of the evolution. However, a more recent duplication of apolipoprotein C-I gene dating from 40 millions years, may be a phylogenetic marker for the radiation of Monkeys. Pancreatic lipase and isoforms, lipoprotein-lipase and hepatic triacylglycerol-lipase form by their homologies a "superfamily" of genes, which also includes yolk proteins of Dipterians eggs. Sequence homologies of PL, LPL and HL are analysed and compared with multiple alignments of amino-acids and nucleotides on spreadsheets. From these comparisons we may characterize four classes of phylogenetic markers: 1) repetitive DNA sequence (Alu, B1, PRE-1) appeared during Mammals evolution, 2) short insertions or deletions (within N-terminal domain) and a gene conversion in guinea-pig lineage, 3) a progressive reduction of intron number during the lipases evolution, 4) several duplications of genes which have produced the five genes of this superfamily currently known in the human genome.
authors.affiliation: Laboratoire des Lipides et des Lipoprotéines, CHU Pitié-Salpêtrère, Paris.
authors.forename: A
authors.initials: A
authors.lastname: Raisonnier
authors.forename: D
authors.initials: D
authors.lastname: Brault
authors.forename: L
authors.initials: L
authors.lastname: Noe
authors.forename: J
authors.initials: J
authors.lastname: Etienne
authors.forename: J
authors.initials: J
authors.lastname: Hamelin
authors.forename: J C
authors.initials: JC
authors.lastname: Chuat
authors.forename: I
authors.initials: I
authors.lastname: Dugail
authors.forename: A
authors.initials: A
authors.lastname: Quignard-Boulange
authors.forename: M
authors.initials: M
authors.lastname: Lavau
authors.forename: F
authors.initials: F
authors.lastname: Galibert
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001053:Apolipoproteins / Q000235:genetics / Q000648:ultrastructure; D001483:Base Sequence; D008049:Lipase / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012689:Sequence Homology, Nucleic Acid
chemical_list: D001053:Apolipoproteins; D008049:Lipase
vernacular_title: Les gènes de la famille des lipases: comparaison des séquences nucléiques et protéiques.