title: Yeast pheromone receptor endocytosis and hyperphosphorylation are independent of G protein-mediated signal transduction.
abstract: When alpha factor binds to the yeast alpha factor receptor a signal is transmitted via a tripartite G protein that prepares the cell for conjugation. As a result of alpha factor binding the receptor also undergoes a regulated internalization and hyperphosphorylation. Using cells that lack activity of this tripartite G protein, we show that G protein-mediated pheromone signal transduction is not necessary for regulation of receptor internalization or hyperphosphorylation. Therefore, the processes of signal transduction and down regulation can be uncoupled. We propose that binding of alpha factor to its receptor results in a receptor conformation change that permits receptor hyperphosphorylation and interaction with the endocytic machinery.
authors.affiliation: Biocenter of the University of Basel, Switzerland.
authors.forename: B
authors.initials: B
authors.lastname: Zanolari
authors.forename: S
authors.initials: S
authors.lastname: Raths
authors.forename: B
authors.initials: B
authors.lastname: Singer-Krüger
authors.forename: H
authors.initials: H
authors.lastname: Riezman
mesh_terms: D015536:Down-Regulation; D004705:Endocytosis; D019204:GTP-Binding Proteins / Q000502:physiology*; D000072235:Mating Factor; D010449:Peptide Mapping; D010455:Peptides / Q000378:metabolism*; D010750:Phosphoproteins / Q000378:metabolism; D010766:Phosphorylation; D011487:Protein Conformation; D011956:Receptors, Cell Surface / Q000502:physiology*; D051964:Receptors, Mating Factor; D018000:Receptors, Peptide; D012441:Saccharomyces cerevisiae / Q000502:physiology*; D015398:Signal Transduction; D014157:Transcription Factors
chemical_list: D010455:Peptides; D010750:Phosphoproteins; D011956:Receptors, Cell Surface; D051964:Receptors, Mating Factor; D018000:Receptors, Peptide; D014157:Transcription Factors; D000072235:Mating Factor; D019204:GTP-Binding Proteins