Pilocarpine-induced increases in the activity of 6-phosphofructo-2-kinase and the fructose-2,6-bisphosphate content of rat salivary glands.
The activity of 6-phosphofructo-1-kinase (PFK-1), an important regulatory enzyme of glycolysis, was determined after injection of the sialagogue pilocarpine. The fructose-2,6-bisphosphate content of the glands and 6-phosphofructo-2-kinase (PFK-2) activity were also measured. The increase in PFK-1 activity after pilocarpine treatment was likely to be due to the increase in the content of its potent modulator, fructose-2,6-bisphosphate. This in turn was assumed to be due to the increase in the activity of the active form of PFK-2.
Nicolau
J
J
Department of Biochemistry, University of São Paulo, Brazil.
de Souza
D N
DN
Martins
H R
HR
D000818:Animals; D005635:Fructosediphosphates / Q000032:analysis*; D006003:Glycogen / Q000032:analysis; D008297:Male; D010306:Parotid Gland / Q000737:chemistry* / Q000187:drug effects* / Q000201:enzymology; D010732:Phosphofructokinase-1 / Q000378:metabolism*; D025481:Phosphofructokinase-2; D010770:Phosphotransferases / Q000378:metabolism*; D010862:Pilocarpine / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D013363:Submandibular Gland / Q000737:chemistry* / Q000187:drug effects* / Q000201:enzymology; D013997:Time Factors
D005635:Fructosediphosphates; D010862:Pilocarpine; C027652:fructose 2,6-diphosphate; D006003:Glycogen; D010770:Phosphotransferases; D025481:Phosphofructokinase-2; D010732:Phosphofructokinase-1