Sodium pump activity in uremic erythrocytes: a microcalorimetric study.
Erythrocyte thermogenesis was studied by flow microcalorimetry in 25 healthy subjects and 27 uremic patients. The heat production (HP) from cells in plasma, decrease in HP induced by ouabain (a specific sodium pump inhibitor) and index of rate response to ouabain action were measured. HP was higher in uremic patients than controls. Sodium pump inhibition with ouabain induced the same decrease in HP in the two groups. The index of rate response to ouabain action was lower in uremic patients than in controls. The difference in total HP may be due to a different age distribution of erythrocytes. Mean sodium pump activity was identical in the two groups, but some patients had lower activity than controls. Ouabain seems to act more slowly in many patients than in controls, perhaps because of hindered binding of the inhibitor.
Gallice
P
P
Laboratory of Biophysics, UFR de Pharmacie, Marseille, France.
Kovacic
H
H
Baz
M
M
Berland
Y
Y
Elsen
R
R
Sari
J C
JC
Crevat
A
A
D002151:Calorimetry / Q000295:instrumentation / Q000379:methods; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D010042:Ouabain / Q000494:pharmacology; D000254:Sodium-Potassium-Exchanging ATPase / Q000187:drug effects / Q000502:physiology*; D014511:Uremia / Q000097:blood*; D014680:Veins
D010042:Ouabain; D000254:Sodium-Potassium-Exchanging ATPase