Frequent amplification of the c-met gene in scirrhous type stomach cancer.
Amplification of the c-met gene, that encodes hepatocyte growth factor receptor, was examined on human esophageal, gastric and colorectal carcinomas. Six (55%) of the 11 gastric carcinoma cell lines and 15 (23%) of the 64 advanced gastric carcinomas showed the c-met gene amplification. Among them, c-met amplification was detected in 5 gastric cancer cell lines, derived from scirrhous gastric carcinoma and in 5 (38%) of 13 scirrhous gastric carcinoma tissues. Furthermore, patients of gastric carcinoma with c-met amplification showed significantly advanced tumor stage and poorer prognosis than those without the amplification. Conversely, no amplification was detected in any of the esophageal and colorectal carcinoma cell lines as well as carcinoma tissues except one colonic carcinoma. These results overall suggest that amplification of the c-met gene might participate in carcinogenesis and progression of stomach cancer, especially scirrhous type stomach carcinoma.
Kuniyasu
H
H
Department of Pathology, Hiroshima University, School of Medicine, Japan.
Yasui
W
W
Kitadai
Y
Y
Yokozaki
H
H
Ito
H
H
Tahara
E
E
D002293:Adenocarcinoma, Scirrhous / Q000235:genetics* / Q000473:pathology; D003110:Colonic Neoplasms / Q000235:genetics; D015342:DNA Probes; D004273:DNA, Neoplasm / Q000235:genetics* / Q000302:isolation & purification; D004938:Esophageal Neoplasms / Q000473:pathology; D005784:Gene Amplification; D017228:Hepatocyte Growth Factor / Q000378:metabolism; D006801:Humans; D011505:Protein-Tyrosine Kinases / Q000235:genetics; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D019859:Proto-Oncogene Proteins c-met; D011519:Proto-Oncogenes; D011956:Receptors, Cell Surface / Q000235:genetics*; D012004:Rectal Neoplasms / Q000235:genetics; D015183:Restriction Mapping; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology; D014407:Tumor Cells, Cultured
D015342:DNA Probes; D004273:DNA, Neoplasm; D011518:Proto-Oncogene Proteins; D011956:Receptors, Cell Surface; D017228:Hepatocyte Growth Factor; D011505:Protein-Tyrosine Kinases; D019859:Proto-Oncogene Proteins c-met