title: Mitochondrial DNA sequence analysis of the cytochrome oxidase subunit I and II genes, the ATPase9 gene, the NADH dehydrogenase ND4L and ND5 gene complex, and the glutaminyl, methionyl and arginyl tRNA genes from Trichophyton rubrum.
abstract: In this paper, we present the nucleotide sequence of a 5248 bp-long region of the mitochondrial (mt) genome of the dermatophyte Trichophyton rubrum. This region which represents about 1/4 of the total mt genome of this species reveals a compact organization of genes including: the glutaminyl tRNA, the methionyl tRNA, the cytochrome oxidase subunit I gene, the arginyl tRNA, the mitochondrial version of the ATPase subunit 9 gene, the cytochrome oxidase subunit II gene and a part of the NADH dehydrogenase ND4L and ND5 gene "complex". The main features of the part of mt DNA sequenced is the non-interrupted COXI gene and the presence in the mitochondrial version of the ATPase 9 gene of a small group IA intron. The extensive amino-acid sequence similarity with the equivalent gene in Aspergillus nidulans and Neuropora crassa indicates that this gene codes for a dicyclohexylcarbodiimide binding protein. The conserved arrangement of this portion of the mt genome and the presence of tRNAs between the protein-coding genes are compatible with a large polycistronic transcript processed by the excision of tRNAs, or similar secondary structures, as proposed for other fungal or mammalian mt DNAS.
authors.affiliation: Unité de Mycologie, Institut Pasteur, Paris, France.
authors.forename: C
authors.initials: C
authors.lastname: de Bièvre
authors.forename: B
authors.initials: B
authors.lastname: Dujon
mesh_terms: D000251:Adenosine Triphosphatases / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D004271:DNA, Fungal / Q000235:genetics; D004272:DNA, Mitochondrial / Q000235:genetics*; D003576:Electron Transport Complex IV / Q000235:genetics*; D005800:Genes, Fungal; D007438:Introns; D008969:Molecular Sequence Data; D009245:NADH Dehydrogenase / Q000235:genetics*; D009690:Nucleic Acid Conformation; D012331:RNA, Fungal / Q000235:genetics; D012346:RNA, Transfer, Amino Acyl / Q000235:genetics*; D012358:RNA, Transfer, Met / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D014249:Trichophyton / Q000201:enzymology / Q000235:genetics*
chemical_list: D004271:DNA, Fungal; D004272:DNA, Mitochondrial; D012331:RNA, Fungal; D012346:RNA, Transfer, Amino Acyl; D012358:RNA, Transfer, Met; D009245:NADH Dehydrogenase; D003576:Electron Transport Complex IV; D000251:Adenosine Triphosphatases