LAN-1 human neuroblastoma cells are provided of endothelin-1 receptors linked to [Ca++]i elevation.
Endothelin-1 (ET-1) produced a dose-dependent increase of intracellular Ca++ concentrations [Ca++]i characterized by an early peak phase and a delayed plateau in LAN-1 human neuroblastoma cells. The ET-1 receptor showed a rapid desensitization since a second pulse application of ET-1 did not elicit a further [Ca++]i increase. Furthermore thapsigargin, an endoplasmic reticulum Ca(++)-ATPase inhibitor, completely abolished the ET-1 induced intracellular Ca++ elevation.
Fatatis
A
A
Department of Human Communication Sciences, 2nd School of Medicine, Federico II University of Naples, Italy.
Bassi
A
A
Mita
G
G
Di Renzo
G F
GF
Annunziato
L
L
D002118:Calcium / Q000378:metabolism*; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D016232:Endothelins / Q000494:pharmacology*; D006801:Humans; D007424:Intracellular Fluid / Q000187:drug effects* / Q000502:physiology; D008856:Microscopy, Fluorescence; D009447:Neuroblastoma; D011956:Receptors, Cell Surface / Q000187:drug effects* / Q000502:physiology; D017466:Receptors, Endothelin; D014407:Tumor Cells, Cultured / Q000187:drug effects*
D016232:Endothelins; D011956:Receptors, Cell Surface; D017466:Receptors, Endothelin; D002118:Calcium