title: Synthesis of a biospecific adsorbent for the purification of the three human retinoic acid receptors by affinity chromatography.
abstract: The total synthesis of an affinity gel suitable for the purification of retinoic acid receptors (hRARs) is reported. A chalcone derived from a potent retinobenzoic acid (Ch55) was chosen as the ligand and fixed to an immobilized matrix by coupling with the N-hydroxysuccinimide ester of agarose (Affi-Gel 10, Bio-Rad Laboratories). Efficiencies of purification of the different human RARs were tested, using recombinant receptors produced with the baculovirus expression system.
authors.affiliation: Unité 16 INSERM, Laboratoire de Biochimie des Protéines, Lille, France.
authors.forename: W
authors.initials: W
authors.lastname: Bourguet
authors.forename: B
authors.initials: B
authors.lastname: Sablonnière
authors.forename: P
authors.initials: P
authors.lastname: Formstecher
authors.forename: J Y
authors.initials: JY
authors.lastname: Chen
authors.forename: J L
authors.initials: JL
authors.lastname: Bernier
authors.forename: J P
authors.initials: JP
authors.lastname: Hénichart
mesh_terms: D002352:Carrier Proteins / Q000302:isolation & purification*; D002599:Chalcone / Q000031:analogs & derivatives* / Q000737:chemistry; D047188:Chalcones; D002846:Chromatography, Affinity; D006801:Humans; D015394:Molecular Structure; D018168:Receptors, Retinoic Acid; D011994:Recombinant Proteins / Q000302:isolation & purification; D012685:Sepharose / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D002352:Carrier Proteins; D047188:Chalcones; D018168:Receptors, Retinoic Acid; D011994:Recombinant Proteins; D002599:Chalcone; C032392:Affi-Gel 10; D012685:Sepharose; C054189:3,5-di-tert-butylchalcone 4'-carboxylic acid