A downstream sequence of the rpL32 promoter competes with the glucocorticoid responsive element for a protein factor.
The murine ribosomal protein (rp) L32 gene contains essential promoter sequences located both upstream and downstream of the cap site. A combination of gel mobility shift, UV cross-linking, and cell-free transcription assays were used to analyze the interaction of factors binding to a downstream element (located at position +25 to +37). The rpL32 downstream element identified polypeptides (transcription factors) ranging in size from 45 to 25 kilodaltons (kDa). Four base pair changes in the wild-type sequence of the downstream element eliminated binding. An oligonucleotide containing the glucocorticoid responsive element sequence competed specifically for the 45-kDa protein in both the gel mobility shift assay and in the UV cross-linking studies. Our data also indicate that the downstream binding factors contribute to cell-free transcription of the rpL32 gene.
Yoganathan
T
T
Department of Molecular Biology and Genetics, College of Biological Science, University of Guelph, Ont., Canada.
Sells
B H
BH
D000818:Animals; D001483:Base Sequence; D001667:Binding, Competitive / Q000502:physiology; D002474:Cell-Free System; D004251:DNA Transposable Elements / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology; D005938:Glucocorticoids / Q000502:physiology*; D051379:Mice; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011485:Protein Binding; D012269:Ribosomal Proteins / Q000235:genetics*; D014157:Transcription Factors / Q000378:metabolism*; D014158:Transcription, Genetic / Q000235:genetics; D014407:Tumor Cells, Cultured; D014466:Ultraviolet Rays
D004251:DNA Transposable Elements; D005938:Glucocorticoids; D015345:Oligonucleotide Probes; D012269:Ribosomal Proteins; D014157:Transcription Factors; C041806:ribosomal protein L32