title: In situ demonstration of Epstein-Barr virus in intravenous drug abusers with generalized lymphadenopathy.
abstract: We have studied by the in situ hybridization method the presence of Epstein-Barr virus (EBV) DNA genome in lymph node tissues from 11 patients with persistent generalized lymphadenopathy. Using a biotinylated EBV DNA probe, we demonstrated EBV nucleic acid in scattered germinal centre cells in eight of the 11 cases. Our results suggest that EBV is not a determinant factor in the pathogenesis of this lymphadenopathy, but support its possible implication in B cell malignant transformation in cases of AIDS-associated lymphoma.
authors.affiliation: Department of Pathology, Hospital Ramon y Cajal, Madrid, Spain.
authors.forename: F
authors.initials: F
authors.lastname: Mampaso
authors.forename: C
authors.initials: C
authors.lastname: Bellas
authors.forename: A
authors.initials: A
authors.lastname: Molina
authors.forename: C
authors.initials: C
authors.lastname: Quereda
authors.forename: T
authors.initials: T
authors.lastname: Bricio
authors.forename: L
authors.initials: L
authors.lastname: Buzon
mesh_terms: D000386:AIDS-Related Complex / Q000382:microbiology*; D004279:DNA, Viral / Q000032:analysis; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D017403:In Situ Hybridization; D008206:Lymphatic Diseases / Q000382:microbiology*; D008297:Male; D015819:Substance Abuse, Intravenous / Q000382:microbiology*
chemical_list: D004279:DNA, Viral