title: Vasoactive intestinal peptide enhances dopamine accumulation in primary cell culture of rat hypothalamus.
abstract: The effect of vasoactive intestinal peptide (VIP) and PHI-27 on dopamine accumulation in cultured rat hypothalamic cells was investigated. VIP enhanced [3H]dopamine accumulation dose dependently. This effect was significant at 10(-8)-10(-5) M VIP with a concomitant increase in intracellular cyclic AMP (cAMP), and reached its plateau level at 10(-6) M VIP. VIP increased [3H]dopamine accumulation significantly within 15 min. PHI-27 and dibutyryl cAMP ((Bu)2-cAMP) also enhanced [3H]dopamine accumulation. These results suggest that VIP enhances dopamine accumulation in hypothalamic cells by increasing intracellular cAMP.
authors.affiliation: Department of Obstetrics and Gynecology, Osaka University Medical School, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Kadowaki
authors.forename: K
authors.initials: K
authors.lastname: Koike
authors.forename: K
authors.initials: K
authors.lastname: Hirota
authors.forename: M
authors.initials: M
authors.lastname: Ohmichi
authors.forename: H
authors.initials: H
authors.lastname: Kurachi
authors.forename: A
authors.initials: A
authors.lastname: Miyoke
authors.forename: O
authors.initials: O
authors.lastname: Tanizawa
mesh_terms: D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000378:metabolism; D004298:Dopamine / Q000378:metabolism*; D005260:Female; D007031:Hypothalamus / Q000166:cytology / Q000378:metabolism*; D010451:Peptide PHI / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D014660:Vasoactive Intestinal Peptide / Q000502:physiology*
chemical_list: D010451:Peptide PHI; D014660:Vasoactive Intestinal Peptide; D003994:Bucladesine; D000242:Cyclic AMP; D004298:Dopamine