title: Probing protein structure by solvent perturbation of nuclear magnetic resonance spectra. Nuclear magnetic resonance spectral editing and topological mapping in proteins by paramagnetic relaxation filtering.
abstract: Soluble spin labels, which "bleach" the surface proton resonances of a protein to n.m.r. measurements, can provide useful information about protein conformation and dynamics. The use of the soluble nitroxide, TEMPOL, has been explored to show the correlation of the paramagnetic perturbations of protein two-dimensional n.m.r. data with proton exposure to the free radical in hen egg-white lysozyme. The results demonstrate that the nitroxide approaches the protein randomly, and that the extent of the observed paramagnetic effects reflects the native folding pattern of the protein. A correlation of spectral simplification with the known tertiary structure establishes the feasibility of new strategies for topological mapping of surface and buried protons of the protein. Application to the elucidation of protein structure and to the study of dynamical processes is discussed.
authors.affiliation: Instituto di Biologia, Facoltà di Medicina, Università di Udine, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Esposito
authors.forename: A M
authors.initials: AM
authors.lastname: Lesk
authors.forename: H
authors.initials: H
authors.lastname: Molinari
authors.forename: A
authors.initials: A
authors.lastname: Motta
authors.forename: N
authors.initials: N
authors.lastname: Niccolai
authors.forename: A
authors.initials: A
authors.lastname: Pastore
mesh_terms: D000595:Amino Acid Sequence; D003497:Cyclic N-Oxides; D004578:Electron Spin Resonance Spectroscopy; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D015335:Molecular Probes; D008969:Molecular Sequence Data; D009113:Muramidase / Q000737:chemistry / Q000187:drug effects; D010449:Peptide Mapping / Q000379:methods; D011485:Protein Binding / Q000187:drug effects; D011487:Protein Conformation / Q000187:drug effects; D012997:Solvents; D013113:Spin Labels; D014867:Water / Q000737:chemistry*
chemical_list: D003497:Cyclic N-Oxides; D015335:Molecular Probes; D012997:Solvents; D013113:Spin Labels; D014867:Water; D009113:Muramidase; C001803:tempol