pp39mos is associated with p34cdc2 kinase in c-mosxe-transformed NIH 3T3 cells.
We investigated the possible interactions between pp39mos and p34cdc2 kinase in NIH 3T3 cells transformed by c-mosxe. pp39mos is coprecipitated with p34cdc2 when using either anti-PSTAIR antibody or p13suc1-Sepharose beads. Likewise, p34cdc2 is coprecipitated with pp39mos when using anti-mos antibody. However, pp39mos was not present in histone H1 kinase-active p34cdc2 complexes precipitated with anti-p34cdc2 C-terminal peptide antibody even during metaphase of the cell cycle. The molar ratio of p34 to pp39mos in the p13suc1 complex is approximately 2:1. Consistent with the tight association between pp39mos and tubulin, tubulin was also present in equivalent amounts with pp39mos and p34 in the p13suc1 complex. This pp39mos-p34cdc2-tubulin complex may be important in transformation by the mos oncogene.
Zhou
R
R
ABL-Basic Research Program, PRI/DynCorp, Inc., Frederick, Maryland.
Daar
I
I
Ferris
D K
DK
White
G
G
Paules
R S
RS
Vande Woude
G
G
D016475:3T3 Cells; D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies; D016203:CDC2 Protein Kinase / Q000032:analysis / Q000378:metabolism*; D002461:Cell Line, Transformed; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D005455:Fluorescent Antibody Technique; D007700:Kinetics; D051379:Mice; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology; D006656:Protamine Kinase / Q000378:metabolism; D011485:Protein Binding; D011505:Protein-Tyrosine Kinases / Q000235:genetics; D011518:Proto-Oncogene Proteins / Q000032:analysis / Q000235:genetics* / Q000378:metabolism*; D017231:Proto-Oncogene Proteins c-mos; D011519:Proto-Oncogenes; D014404:Tubulin / Q000378:metabolism
D000906:Antibodies; D010455:Peptides; D011518:Proto-Oncogene Proteins; D014404:Tubulin; D011505:Protein-Tyrosine Kinases; D006656:Protamine Kinase; D017231:Proto-Oncogene Proteins c-mos; D016203:CDC2 Protein Kinase