Studies on p40, the leucine zipper motif-containing protein encoded by the first open reading frame of an active human LINE-1 transposable element.
Full-length human LINE-1 retrotransposons encode p40 proteins with varying electrophoretic mobilities under denaturing conditions. The p40 expressed from the first open reading frame in the LINE-1 copy designated L1.2A co-electrophoreses with the endogenous p40 in human teratocarcinoma cells. This finding is consistent with previous data indicating that L1.2A is an active element. The amino acid sequence in the central region of the L1.2A p40 accounts, at least in part, for its characteristic mobility. This region includes sequences which can, in principle, form a leucine zipper.
Holmes
S E
SE
Laboratory of Biochemistry, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892.
Singer
M F
MF
Swergold
G D
GD
D000595:Amino Acid Sequence; D001483:Base Sequence; D015153:Blotting, Western; D004251:DNA Transposable Elements; D004268:DNA-Binding Proteins; D006801:Humans; D016350:Leucine Zippers / Q000235:genetics*; D008969:Molecular Sequence Data; D009841:Oligonucleotides; D016366:Open Reading Frames; D010455:Peptides / Q000235:genetics*; D014176:Protein Biosynthesis; D011506:Proteins / Q000235:genetics*; D013724:Teratoma / Q000378:metabolism; D014407:Tumor Cells, Cultured
D004251:DNA Transposable Elements; D004268:DNA-Binding Proteins; D009841:Oligonucleotides; D010455:Peptides; D011506:Proteins; C077214:L1Hs-encoded protein p40, human