Affinity chromatographic purification of bovine lung endothelin receptor using biotinylated endothelin and avidin-agarose.
Endothelin receptor was purified from bovine lung by affinity chromatography using biotinylated endothelin and avidin-agarose. Endothelin was biotinylated with sulphosuccinimidyl 2-(biotinamido)ethyl-1,3-dithiopropionate, a reactive form of biotin with a cleavable spacer arm containing a disulphide bond designed for a simple elution by thiols. Starting from 3.5 kg of bovine lung, about 200 micrograms of pure receptor were obtained.
Hagiwara
H
H
Department of Biological Sciences, Tokyo Institute of Technology, Japan.
Nagasawa
T
T
Lodhi
K M
KM
Kozuka
M
M
Ito
T
T
Hirose
S
S
D000595:Amino Acid Sequence; D000818:Animals; D001360:Avidin; D001710:Biotin; D002417:Cattle; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D016232:Endothelins; D008168:Lung / Q000737:chemistry*; D008969:Molecular Sequence Data; D011956:Receptors, Cell Surface / Q000302:isolation & purification*; D017466:Receptors, Endothelin; D012685:Sepharose
D016232:Endothelins; D011956:Receptors, Cell Surface; D017466:Receptors, Endothelin; D001360:Avidin; D001710:Biotin; D012685:Sepharose