Dual effects of endothelin-1 on neurite outgrowth induced by 12-O-tetradecanoylphorbol-13-acetate.
The vasoactive peptide endothelin-1 (ET-1) was found to bind to a single class of binding sites in chick embryonic sensory ganglia with a Kd of 67 +/- 5 pM. Treatment of ganglia explants with 100 pM ET-1 did not affect neuronal development, but when added together with 12-O-tetradecanoylphorbol-13-acetate (TPA) a synergistic stimulation of neurite outgrowth was observed. In contrast, 10 nM ET-1 inhibited TPA-induced neurite outgrowth. Both the stimulatory and inhibitory effects were not blocked by nifedipine, a Ca2+ channel blocker. These results suggest that ET-1 can modulate the process of neurite outgrowth and its effects are not dependent on voltage-gated Ca2+ channels.
Hsu
L
L
Department of Biology, Seton Hall University, South Orange, NJ 07079.
Savage
P
P
Jeng
A Y
AY
D000818:Animals; D002118:Calcium / Q000502:physiology; D015220:Calcium Channels / Q000187:drug effects; D002642:Chick Embryo; D016232:Endothelins / Q000494:pharmacology*; D005724:Ganglia / Q000187:drug effects / Q000254:growth & development / Q000502:physiology; D007700:Kinetics; D016501:Neurites / Q000187:drug effects*; D009543:Nifedipine / Q000494:pharmacology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D015220:Calcium Channels; D016232:Endothelins; D009543:Nifedipine; D013755:Tetradecanoylphorbol Acetate; D002118:Calcium