Protection against tabun toxicity in mice by prophylaxis with an enzyme hydrolyzing organophosphate esters.
We demonstrate here the correlation between protection afforded by pretreatment alone with parathion hydrolase purified from Pseudomonas sp. against tabun toxicity in mice and the kinetic parameters which are assumed to determine the in vivo detoxification of tabun by the same enzyme. Results show that 15 and 22 micrograms of parathion hydrolase per animal conferred a protective ratio of 3.94 and 5.65 respectively, against tabun toxicity, without post-exposure treatment.
Raveh
L
L
Israel Institute for Biological Research, Ness-Ziona, Israel.
Segall
Y
Y
Leader
H
H
Rothschild
N
N
Levanon
D
D
Henis
Y
Y
Ashani
Y
Y
D000818:Animals; D043303:Aryldialkylphosphatase; D002800:Cholinesterase Inhibitors / Q000633:toxicity*; D005459:Fluorides / Q000037:antagonists & inhibitors; D007700:Kinetics; D051379:Mice; D010755:Organophosphates / Q000037:antagonists & inhibitors* / Q000633:toxicity; D010278:Parathion / Q000037:antagonists & inhibitors; D010710:Phosphates / Q000037:antagonists & inhibitors; D010744:Phosphoric Monoester Hydrolases / Q000008:administration & dosage* / Q000302:isolation & purification / Q000494:pharmacology; D011549:Pseudomonas / Q000201:enzymology
D002800:Cholinesterase Inhibitors; D010755:Organophosphates; D010710:Phosphates; C012980:fluorophosphate; D010278:Parathion; D010744:Phosphoric Monoester Hydrolases; D043303:Aryldialkylphosphatase; D005459:Fluorides; C009374:tabun