Insulin and progesterone increase 32PO4-labeling of phospholipids and inositol 1,4,5-trisphosphate mass in Xenopus oocytes.
After a 4-6 h induction period, insulin or progesterone induces Xenopus oocytes to enter prophase of meiosis. During the period of induction, both insulin and progesterone induced an increase in 32PO4 labeling of phosphatidylcholine and phosphatidylinositol. Through a mass assay, we found that insulin and progesterone increase inositol 1,4,5-trisphosphate (IP3) at about 15-30 s, 15 min and at about 2-3 h (0.5 GVBD50) after hormone addition. Since IP3 increases were small (from a basal of 66 to 104 nM), the results agree with prior conclusions that progesterone does not induce a large, cytosolic calcium elevation. Insulin is probably acting through the insulin-like growth factor-1 receptor as insulin concentrations greater than about 50 nM are required to increase IP3.
Stith
B J
BJ
Department of Biology, University of Colorado, Denver.
Jaynes
C
C
Goalstone
M
M
Silva
S
S
D000818:Animals; D015220:Calcium Channels; D005260:Female; D015544:Inositol 1,4,5-Trisphosphate / Q000378:metabolism*; D053496:Inositol 1,4,5-Trisphosphate Receptors; D007328:Insulin / Q000494:pharmacology*; D007334:Insulin-Like Growth Factor I / Q000378:metabolism; D008540:Meiosis / Q000187:drug effects / Q000502:physiology; D009865:Oocytes / Q000187:drug effects* / Q000378:metabolism; D010710:Phosphates / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism*; D010758:Phosphorus / Q000378:metabolism; D010761:Phosphorus Radioisotopes; D011374:Progesterone / Q000494:pharmacology*; D011956:Receptors, Cell Surface / Q000378:metabolism; D018160:Receptors, Cytoplasmic and Nuclear; D017451:Receptors, Somatomedin; D014981:Xenopus
D015220:Calcium Channels; D053496:Inositol 1,4,5-Trisphosphate Receptors; D007328:Insulin; D010710:Phosphates; D010743:Phospholipids; D010761:Phosphorus Radioisotopes; D011956:Receptors, Cell Surface; D018160:Receptors, Cytoplasmic and Nuclear; D017451:Receptors, Somatomedin; D010758:Phosphorus; D011374:Progesterone; D007334:Insulin-Like Growth Factor I; D015544:Inositol 1,4,5-Trisphosphate