Glutathione mobilization during cerebral ischemia and reperfusion in the rat.
1. Cerebral ischemia applied for 15 min and followed by a 30 min reperfusion did not change the glutathione (GSH) levels and beta-adrenoceptor density (Bmax) in brain cortex. 2. A significant increase in erythrocyte-lysate GSH concentration (vs control) and a significant decrease of Bmax values in erythrocyte membranes (vs control) was found at the same time. 3. Pretreatment with the alpha-adrenoceptor antagonist phentolamine (5 mg/kg i.p.) prevented the erythrocyte GSH increase but not the decrease of Bmax value. Pretreatment with the beta-antagonist propranolol (2 mg/kg i.p.) did not influence the increase in erythrocyte GSH but circumvented the decrease of Bmax.
Kramer
K
K
Department of Pharmacochemistry, Faculty of Chemistry, Vrije Universiteit, Amsterdam, The Netherlands.
Voss
H P
HP
Grimbergen
J A
JA
Smink
C
C
Timmerman
H
H
Bast
A
A
D000818:Animals; D002545:Brain Ischemia / Q000378:metabolism*; D002540:Cerebral Cortex / Q000378:metabolism*; D015536:Down-Regulation / Q000187:drug effects; D004912:Erythrocytes / Q000378:metabolism; D005978:Glutathione / Q000097:blood / Q000378:metabolism*; D008297:Male; D010646:Phentolamine / Q000494:pharmacology; D011433:Propranolol / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D011943:Receptors, Adrenergic, beta / Q000378:metabolism*; D015424:Reperfusion
D011943:Receptors, Adrenergic, beta; D011433:Propranolol; D005978:Glutathione; D010646:Phentolamine