title: Liquid chromatographic mass spectrometric methods for the determination of marine polyether toxins.
abstract: Experiments with novel nitrogenous coumarin-based reagents yielded moderately fluorescent derivatives of brevetoxin-3 and ciguatoxin-1. The diethylaminocoumarin-carbamic acid esters of brevetoxin-3 were resolved by high performance liquid chromatography into two derivative peaks that correspond to substitutions at the C-37 and C-41 hydroxyls. The derivatives produced intense molecular ions under fast atom bombardment ionization conditions, confirming derivative identity. Ciguatoxin-1 was also successfully derivatized, resolved, and identified by HPLC-fluorometry with an estimated lower limit of detection of 0.5 to 1.0 ng.
authors.affiliation: US Food and Drug Administration, Division of Seafood Research, Dauphin Island, AL 36528.
authors.forename: R W
authors.initials: RW
authors.lastname: Dickey
authors.forename: F A
authors.initials: FA
authors.lastname: Bencsath
authors.forename: H R
authors.initials: HR
authors.lastname: Granade
authors.forename: R J
authors.initials: RJ
authors.lastname: Lewis
mesh_terms: D002851:Chromatography, High Pressure Liquid; D002922:Ciguatoxins / Q000032:analysis; D003374:Coumarins; D004988:Ethers, Cyclic / Q000032:analysis; D005456:Fluorescent Dyes; D008387:Marine Toxins / Q000032:analysis*; D009550:Nigericin / Q000032:analysis; D019319:Okadaic Acid; D035964:Oxocins; D016339:Spectrometry, Mass, Fast Atom Bombardment; D000095702:Polyether Toxins
chemical_list: D002922:Ciguatoxins; D003374:Coumarins; D004988:Ethers, Cyclic; D005456:Fluorescent Dyes; D008387:Marine Toxins; D009550:Nigericin; D019319:Okadaic Acid; D035964:Oxocins; C053342:brevetoxin; D000095702:Polyether Toxins