Respiratory chain defect of myocardial mitochondria in idiopathic dilated cardiomyopathy of Doberman pinscher dogs.
Idiopathic dilated cardiomyopathy (IDCM) is a primary myocardial disease of unknown cause. We tested the hypothesis that IDCM was associated with a myocardial metabolic defect by determining a comprehensive biochemical profile of metabolite concentrations and enzyme activities for the major metabolic pathways of the myocardium. We used the Doberman pinscher breed as a naturally occurring canine model of IDCM and compared its myocardial profile with that of healthy adult mongrels. Compared with controls, myocardium in IDCM had markedly reduced mitochondrial electron transport activity and myoglobin concentration, in association with acidosis and energy depletion following anoxic challenge: 60% decreased NADH dehydrogenase and 50% decreased ATP synthetase activities; 90% decreased myoglobin concentration; and 30% reduced ATP and 50% increased lactate and proton concentrations. Sarcoplasmic reticulum Ca(2+)-transport ATPase was decreased by 42%. There was a 15% compensatory increase in fatty acid oxidation and Krebs cycle activity. Other biochemical changes were mild by comparison with the mitochondrial defects. We conclude that IDCM is associated with a marked impairment of mitochondrial production of ATP, arising from decreased activity of the mitochondrial electron transport system, including myoglobin. These changes may be secondary to an underlying genetic defect or may indicate a deficiency of the mitochondrial respiratory chain that predisposes this breed to heart failure.
McCutcheon
L J
LJ
Department of Pathology, Ontario Veterinary College, University of Guelph, Canada.
Cory
C R
CR
Nowack
L
L
Shen
H
H
Mirsalami
M
M
Lahucky
R
R
Kovac
L
L
O'Grady
M
M
Horne
R
R
O'Brien
P J
PJ
D025181:ATP Synthetase Complexes; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002311:Cardiomyopathy, Dilated / Q000378:metabolism*; D004195:Disease Models, Animal; D004285:Dogs; D005260:Female; D008297:Male; D008929:Mitochondria, Heart / Q000378:metabolism*; D009097:Multienzyme Complexes / Q000378:metabolism; D009211:Myoglobin / Q000378:metabolism; D009245:NADH Dehydrogenase / Q000378:metabolism; D010770:Phosphotransferases / Q000378:metabolism
D009097:Multienzyme Complexes; D009211:Myoglobin; D000255:Adenosine Triphosphate; D009245:NADH Dehydrogenase; D010770:Phosphotransferases; D025181:ATP Synthetase Complexes