title: A pancreatic-like ribonuclease is synthesized in rat brain.
abstract: The distribution and cell localization of a pancreatic-like ribonuclease (RNAase) in the rat brain has been studied by RNA blot analysis and in situ hybridization using as a probe the cDNA coding for the rat pancreas RNAase, and by immunocytochemistry using an antiserum raised against the rat pancreas RNAase. RNA blot analysis and in situ hybridization experiments have shown that the RNAase mRNA is present in all the cerebral areas investigated and that neurons appeared to be actively expressing RNAase mRNA while glial cells were devoid of hybridization signals. In agreement with these results the immunocytochemical analysis has shown that neurons are specifically immunostained. These experiments demonstrate that a pancreatic-like ribonuclease is synthesized in the neurons of the rat brain.
authors.affiliation: Institute of Cellular Biology, University of Perugia, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Grassi Zucconi
authors.forename: C
authors.initials: C
authors.lastname: Cosi
authors.forename: M
authors.initials: M
authors.lastname: Palmieri
authors.forename: A
authors.initials: A
authors.lastname: Furia
authors.forename: M A
authors.initials: MA
authors.lastname: Bassetti
authors.forename: A
authors.initials: A
authors.lastname: Carsana
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D001921:Brain / Q000201:enzymology*; D004247:DNA / Q000235:genetics; D009419:Nerve Tissue Proteins / Q000096:biosynthesis* / Q000235:genetics; D009474:Neurons / Q000201:enzymology; D010179:Pancreas / Q000201:enzymology; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D012260:Ribonucleases / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D004247:DNA; D012260:Ribonucleases