title: Stoichiometric binding of 2'(or 3')-O-(2,4,6-trinitrophenyl)adenosine 5'-triphosphate to bovine heart cytochrome c oxidase.
abstract: The binding of 2'(or 3')-O-(2,4,6-trinitrophenyl)adenosine 5'-triphosphate (TNP-ATP) to isolated bovine heart cytochrome c oxidase (COX) was studied by following its specific spectral change at 510 nm. The quantitative titration revealed two binding sites for TNP-ATP per monomer COX with a Kd of 1.6 microM.
authors.affiliation: Fachbereich Chemie, Philipps-Universität, Marburg, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Reimann
authors.forename: B
authors.initials: B
authors.lastname: Kadenbach
mesh_terms: D000255:Adenosine Triphosphate / Q000031:analogs & derivatives* / Q000378:metabolism; D000818:Animals; D002417:Cattle; D003576:Electron Transport Complex IV / Q000378:metabolism*; D005456:Fluorescent Dyes; D008929:Mitochondria, Heart / Q000201:enzymology*; D013057:Spectrum Analysis
chemical_list: C009587:2',3'-O-(2,4,6-trinitrophenyl)adenosine 5'-triphosphate; D005456:Fluorescent Dyes; D000255:Adenosine Triphosphate; D003576:Electron Transport Complex IV