Ultrastructural localization of human papilloma virus by nonradioactive in situ hybridization on tissue of human cervical intraepithelial neoplasia.
BACKGROUND
A nonradioactive in situ hybridization was developed to localize human papilloma virus (HPV) at the ultrastructural level.


METHODS
Cervical biopsies from human uterine cervices clinically suspicious of condyloma were embedded in Lowicryl K4M at low temperature. Postembedding in situ hybridization was performed with DNA probes specific for HPV types 6/11, 16, and 18. The hybrids were detected by anti-horseradish peroxidase antibodies conjugated with 10 nm colloidal gold particles.


RESULTS
Localization for HPV 16 and 18 both was to intranuclear and cytoplasmic sites. Cytoplasmic detected HPV signals were between masses of intermediate filaments and in vacuoles; other organelles were devoid of positive signal. Within the nucleus the precise localization of the viral nucleic acid was episomal, vacuolar, and chromosomal. In situ hybridization with plasmid control DNA confirmed the specificity of the HPV positive signals.


CONCLUSIONS
This study helps define the subcellular compartmentalization of HPV DNA in infected human cells.
Multhaupt
H A
HA
Department of Pathology, Pennsylvania Hospital, Philadelphia.
Rafferty
P A
PA
Warhol
M J
MJ
D004848:Epithelium / Q000382:microbiology; D005260:Female; D006046:Gold; D006735:Horseradish Peroxidase; D006801:Humans; D017403:In Situ Hybridization / Q000379:methods*; D027383:Papillomaviridae / Q000648:ultrastructure*; D016613:Plastic Embedding; D013696:Temperature; D002583:Uterine Cervical Neoplasms / Q000382:microbiology*
D006046:Gold; D006735:Horseradish Peroxidase