title: Determination of stavudine, a new antiretroviral agent, in human plasma by reversed-phase high-performance liquid chromatography with ultraviolet detection.
abstract: A sensitive high-performance liquid chromatographic assay has been developed to determine the levels of a new antiretroviral agent, stavudine (2',3'-didehydro-3'-deoxythymidine, d4T), in human plasma. Didanosine (2',3'-dideoxyinosine, ddI) was used as the internal standard. The very selective sample pretreatment involved solid-phase extraction using silica gel columns. Chromatography was carried out on a mu Bondapak phenyl column, using a mobile phase of 0.005 M phosphate buffer (pH 6.8)-methanol (90:10, v/v) and ultraviolet detection at 265 nm. The method has been validated, and stability tests under various conditions have been performed. The detection limit is 10 ng/ml (using 500-microliters human plasma samples). The bioanalytical assay has been used in a single pharmacokinetic experiment in a rat to investigate the applicability of the method in vivo.
authors.affiliation: Department of Pharmacy, Slotervaart Hospital, Amsterdam, Netherlands.
authors.forename: D M
authors.initials: DM
authors.lastname: Burger
authors.forename: H
authors.initials: H
authors.lastname: Rosing
authors.forename: R
authors.initials: R
authors.lastname: van Gijn
authors.forename: P L
authors.initials: PL
authors.lastname: Meenhorst
authors.forename: O
authors.initials: O
authors.lastname: van Tellingen
authors.forename: J H
authors.initials: JH
authors.lastname: Beijnen
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000097:blood* / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D015224:Dideoxynucleosides / Q000097:blood* / Q000493:pharmacokinetics; D006801:Humans; D051381:Rats; D015203:Reproducibility of Results; D012190:Retroviridae; D013056:Spectrophotometry, Ultraviolet; D018119:Stavudine
chemical_list: D000998:Antiviral Agents; D015224:Dideoxynucleosides; D018119:Stavudine