Characterization of H2 histamine receptor: linkage to both adenylate cyclase and [Ca2+]i signaling systems.
We undertook these studies to examine the intracellular mechanisms of histamine action using a rat hepatoma-derived cell line that had been transfected to express the H2 histamine receptor cDNA. Transfected cells demonstrated increased adenosine 3',5'-cyclic monophosphate production, membrane inositol phospholipid turnover, and intracellular Ca2+ concentration ([Ca2+]i) in response to histamine. All of the effects could be inhibited with the H2 histamine receptor antagonist cimetidine, and the increased membrane inositol phospholipid turnover and [Ca2+]i were abolished by cholera toxin pretreatment of cells. These data support the notion that a single histamine H2 receptor can be linked to two stimulatory intracellular signaling systems.
Delvalle
J
J
Department of Internal Medicine, University of Michigan and Medical Center, Ann Arbor 48109.
Wang
L
L
Gantz
I
I
Yamada
T
T
D000262:Adenylyl Cyclases / Q000378:metabolism*; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D000242:Cyclic AMP / Q000096:biosynthesis; D006632:Histamine / Q000502:physiology; D015544:Inositol 1,4,5-Trisphosphate / Q000096:biosynthesis; D007425:Intracellular Membranes / Q000378:metabolism*; D008113:Liver Neoplasms / Q000378:metabolism / Q000473:pathology; D008114:Liver Neoplasms, Experimental / Q000378:metabolism / Q000473:pathology; D051381:Rats; D011970:Receptors, Histamine H2 / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D014162:Transfection; D014407:Tumor Cells, Cultured
D011970:Receptors, Histamine H2; D006632:Histamine; D015544:Inositol 1,4,5-Trisphosphate; D000242:Cyclic AMP; D000262:Adenylyl Cyclases; D002118:Calcium