Effect of PGE2 on interleukin-1 and superoxide release from primary-cultured human hepatic macrophages.
In order to learn more about how human hepatic macrophages function, we analyzed the effect of exogenous PGE2 on the amounts of interleukin-1 (IL-1) and superoxide (O2-) released from primary-cultured human hepatic macrophages (HHM phi). When endogenous PGE2 production was blocked by indomethacin, exogenous PGE2 reduced IL-1 release from HHM phi in a dose-dependent manner, whereas it tended to increase O2- release from HHM phi. These results may suggest the probable contribution of PGE2 in regulating HHM phi mediator release in vivo.
Funaki
N
N
First Department of Surgery, Faculty of Medicine, Kyoto University, Japan.
Arii
S
S
Adachi
Y
Y
Higashituji
H
H
Fujita
S
S
Furutani
M
M
Mise
M
M
Ishiguro
S
S
Kitao
T
T
Tanaka
J
J
D002478:Cells, Cultured; D015232:Dinoprostone / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007213:Indomethacin / Q000494:pharmacology; D007375:Interleukin-1 / Q000378:metabolism*; D008099:Liver / Q000166:cytology* / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism*
D007375:Interleukin-1; D013481:Superoxides; D015232:Dinoprostone; D007213:Indomethacin