Nitric oxide donors increase mucus gel thickness in rat stomach.
Instillation of the nitric oxide (NO) generator isosorbide dinitrate (0.1-1 mM) into the rat gastric lumen in vivo produced a dose-related increase in mucus gel thickness that was prevented by coadministration of oxyhaemoglobin (10 microM). Isosorbide dinitrate did not induce epithelial cell damage. S-Nitroso-N-acetyl-penicillamine (0.3 mM) and dibutyryl cyclic GMP (1 mM) also increased mucus thickness. These findings, along with the presence of NO synthase in the gastric mucosa, imply a role for NO in vivo in mediation of gastric mucus release.
Brown
J F
JF
Pharmaceutical Sciences Institute, Aston University, Birmingham, UK.
Hanson
P J
PJ
Whittle
B J
BJ
D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005753:Gastric Mucosa / Q000187:drug effects*; D007548:Isosorbide Dinitrate / Q000494:pharmacology*; D008297:Male; D009093:Mucus / Q000378:metabolism*; D009569:Nitric Oxide / Q000494:pharmacology*; D010108:Oxyhemoglobins / Q000494:pharmacology; D010396:Penicillamine / Q000031:analogs & derivatives / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D026423:S-Nitroso-N-Acetylpenicillamine
D010108:Oxyhemoglobins; D009569:Nitric Oxide; D003994:Bucladesine; D026423:S-Nitroso-N-Acetylpenicillamine; D010396:Penicillamine; D007548:Isosorbide Dinitrate