Molecular properties of the vasoactive intestinal peptide receptor in aorta and other tissues.
The molecular weight of the vasoactive intestinal peptide (VIP) receptor was assessed in bovine aorta, and rat liver, lung, and brain by covalent cross-linking and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The receptor in all four tissues was found to be a single polypeptide of approximate M(r) 54,000, contradicting previous claims for substantial heterogeneity in the molecular weight of this receptor. Guanine nucleotides inhibit cross-linking of 125I-VIP to its receptor, and cross-linking with ethylene glycolbis(succinimidylsuccinate) provides further evidence for complex formation between VIP, its receptor and a guanine nucleotide-binding regulatory protein (G-protein). The precise mechanism of receptor-G-protein coupling may differ between the aorta and other tissues.
Shreeve
S M
SM
Department of Pharmacology, College of Medicine, University of Vermont, Burlington 05405.
DeLuca
A W
AW
Diehl
N L
NL
Kermode
J C
JC
D000818:Animals; D001011:Aorta / Q000378:metabolism*; D001921:Brain / Q000378:metabolism; D003432:Cross-Linking Reagents; D019204:GTP-Binding Proteins / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008168:Lung / Q000378:metabolism; D008297:Male; D008970:Molecular Weight; D051381:Rats; D017207:Rats, Sprague-Dawley; D011964:Receptors, Gastrointestinal Hormone / Q000737:chemistry* / Q000378:metabolism; D018005:Receptors, Vasoactive Intestinal Peptide; D014018:Tissue Distribution; D014660:Vasoactive Intestinal Peptide / Q000378:metabolism*
D003432:Cross-Linking Reagents; D011964:Receptors, Gastrointestinal Hormone; D018005:Receptors, Vasoactive Intestinal Peptide; D014660:Vasoactive Intestinal Peptide; D019204:GTP-Binding Proteins