Sequence analysis and characterization of the Porphyromonas gingivalis prtC gene, which expresses a novel collagenase activity.
In order to examine the potential role of bacterial collagenases in periodontal tissue destruction, we recently isolated a gene, prtC, from Porphyromonas gingivalis ATCC 53977, which expressed collagenase activity (N. Takahashi, T. Kato, and H. K. Kuramitsu, FEMS Microbiol. Lett. 84:135-138, 1991). The nucleotide sequence of the gene has been determined, and the deduced amino acid sequence corresponds to a basic protein of 37.8 kDa. In addition, Southern blot analysis indicated that the prtC gene is conserved among the three major serotypes of P. gingivalis. The enzyme has been purified to near homogeneity from Escherichia coli clone NTS1 following Mono Q anion exchange and sequential gel filtration chromatography. The molecular mass of the purified enzyme was estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis to be ca. 35 kDa, and the active enzyme behaved as a dimer following gel filtration chromatography. The collagenase degraded soluble and reconstituted fibrillar type I collagen, heat-denatured type I collagen, and azocoll but not gelatin or the synthetic collagenase substrate 4-phenylazobenzyloxycarbonyl-Pro-Leu-Gly-Pro-D-Arg. Enzyme activity was enhanced by Ca2+ and inhibited by EDTA, sulfhydryl-blocking agents, and the salivary peptide histatin. Preliminary evidence for the existence of a second collagenase expressed by strain 53977 was also obtained.
Kato
T
T
Department of Pediatric Dentistry, University of Texas Health Science Center, San Antonio 78284.
Takahashi
N
N
Kuramitsu
H K
HK
D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D017364:Collagenases; D008670:Metals / Q000378:metabolism; D003012:Microbial Collagenase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D016966:Porphyromonas gingivalis / Q000201:enzymology* / Q000235:genetics
D001426:Bacterial Proteins; D008670:Metals; D017364:Collagenases; C074887:prtC protein, bacteria; D003012:Microbial Collagenase