Expression of c-met proto-oncogene in COS cells induces the signal transducing high-affinity receptor for hepatocyte growth factor.
By transfection of the expression plasmid containing a human c-met cDNA into COS-7 cells, high-affinity binding sites specific for HGF with a Kd value of 30 pM were newly detected. Furthermore, only in the c-met transfected COS-7 cells, but not in the control COS-7 cells, DNA synthesis was markedly induced in response to HGF. Thus, transient expression of exogenous c-met cDNA resulted in the appearance of high-affinity receptor for HGF and conversion of the normally non-responsive COS-7 cells into the HGF-responsive cells. These results provide evidence for identifying the c-met product as a signal transducing high-affinity receptor for HGF.
Higuchi
O
O
Department of Biology, Faculty of Science, Kyushu University, Fukuoka, Japan.
Mizuno
K
K
Vande Woude
G F
GF
Nakamura
T
T
D002460:Cell Line; D004247:DNA / Q000235:genetics; D015870:Gene Expression; D006801:Humans; D007457:Iodine Radioisotopes; D008934:Mitogens; D010957:Plasmids; D016133:Polymerase Chain Reaction; D011505:Protein-Tyrosine Kinases / Q000235:genetics*; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D019859:Proto-Oncogene Proteins c-met; D011956:Receptors, Cell Surface / Q000235:genetics*; D015398:Signal Transduction; D014529:Uridine / Q000378:metabolism
D007457:Iodine Radioisotopes; C000715470:MAS1 protein, human; D008934:Mitogens; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins; D011956:Receptors, Cell Surface; D004247:DNA; D011505:Protein-Tyrosine Kinases; D019859:Proto-Oncogene Proteins c-met; D014529:Uridine