Characterization of the glycoprotein D gene products of equine herpesvirus 1 using a prokaryotic cell expression vector.
The gene encoding equine herpesvirus 1 (equine abortion virus; EHV-1) glycoprotein D was engineered into the prokaryotic vector pEX, and expressed as a beta-galactosidase fusion product, which was recognized by pooled equine sera and anti-EHV-1 rabbit sera. Antibodies raised against the EHV-1 gD fusion product identified strong bands in infected cells at 66 and 68 K and at 138 K in purified virus, thus characterizing the several forms of this major envelope glycoprotein which is an important candidate for inclusion in subunit vaccines.
Love
D N
DN
Bell
C W
CW
Whalley
J M
JM
D000818:Animals; D015967:Gene Expression Regulation, Viral; D005822:Genetic Vectors; D004861:Herpesvirus 1, Equid / Q000235:genetics*; D014759:Viral Envelope Proteins / Q000235:genetics*
D014759:Viral Envelope Proteins; C488988:glycoprotein D, Equid herpesvirus 1