title: Mutations that affect the regulation of phs in Salmonella typhimurium.
abstract: The regulation of phs [production of hydrogen sulphide (H2S)] in Salmonella typhimurium is complex. Previous studies have shown that expression is dependent upon the presence of reduced sulphur and anaerobiosis and is modulated by carbon source and growth stage. Transposon mutagenesis failed to find any potential trans-acting factors effective in the regulation of phs in relation to oxygen. Spontaneous mutants capable of expressing phs-lac aerobically were isolated and characterized. These mutations are closely linked to phs and affect not only oxygen regulation but also the requirement for cyclic AMP and reduced sulphur. Analysis of merodiploid strains indicates that these mutations cis-acting and that phs is not subject to autoregulation.
authors.affiliation: Département de microbiologie et immunologie, Université de Montréal, Québec, Canada.
authors.forename: N
authors.initials: N
authors.lastname: Alami
authors.forename: P C
authors.initials: PC
authors.lastname: Hallenbeck
mesh_terms: D000332:Aerobiosis; D000693:Anaerobiosis; D000242:Cyclic AMP / Q000494:pharmacology; D004251:DNA Transposable Elements; D004171:Diploidy; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects*; D005947:Glucose / Q000494:pharmacology; D006862:Hydrogen Sulfide / Q000378:metabolism*; D007763:Lac Operon; D016254:Mutagenesis, Insertional; D009154:Mutation; D010100:Oxygen / Q000494:pharmacology; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics; D012486:Salmonella typhimurium / Q000235:genetics*; D014161:Transduction, Genetic
chemical_list: D004251:DNA Transposable Elements; D011993:Recombinant Fusion Proteins; D000242:Cyclic AMP; D005947:Glucose; D010100:Oxygen; D006862:Hydrogen Sulfide