title: Characterization of L-glutamate and kainate binding sites in the brain of a freshwater fish, Telapilia monsanbica.
abstract: [3H]Kainate and L-[3H]glutamate binding sites in a rich source of kainate binding sites, fish brain, have been thoroughly analysed here for the purpose of studying the correlation between kainate binding sites and L-glutamate receptors in vertebrate CNS. The brain of a freshwater fish, Telapilia monsanbica, was found to contain three types of kainate binding sites: Type 1 sites (Kd = 1050 +/- 380 microM, Bmax = 4 +/- 4 pmol/mg), Type 2 sites (Kd = 133 +/- 20 nM, Bmax = 190 +/- 20 pmol/mg), and Type 3 sites (Kd = 23 +/- 15 nM, Bmax = 28 +/- 19 pmol/mg). The dissociation constants of L-glutamate to Type 1, 2 and 3 sites were, respectively, 0.28 +/- 0.04, 5.5 +/- 0.2 and 137 +/- 28 microM. Pharmacological characterization of these binding sites showed that Type 1 and 2 sites, respectively, corresponded to N-methyl-D-aspartate-subtype L-glutamate receptors and non-N-methyl-D-aspartate L-glutamate receptors. Autoradiographic studies showed that Type 1 and 2 sites were distributed widely in fish brain, indicating the involvement of L-glutamate receptors in various brain functions. Type 3 sites, on the other hand, were relatively insensitive to most endogenous amino acids and were only found in the molecular layer of cerebellum and torus longitudinalis. Type 3 sites possibly representing a distinctive class of receptor has been suggested by the results.
authors.affiliation: Institute of Life Science, National Tsing Hua University, Hsinchu, Taiwan, Republic of China.
authors.forename: C K
authors.initials: CK
authors.lastname: Tong
authors.forename: M P
authors.initials: MP
authors.lastname: Pan
authors.forename: Y C
authors.initials: YC
authors.lastname: Chang
mesh_terms: D000818:Animals; D001345:Autoradiography; D001667:Binding, Competitive; D001921:Brain / Q000166:cytology / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D005399:Fishes; D005618:Fresh Water; D005971:Glutamates / Q000378:metabolism*; D007608:Kainic Acid / Q000378:metabolism*; D007700:Kinetics; D011869:Radioligand Assay; D017470:Receptors, Glutamate / Q000378:metabolism*; D018092:Kainic Acid Receptors; D017981:Receptors, Neurotransmitter / Q000378:metabolism*; D014316:Tritium
chemical_list: D005971:Glutamates; D007608:Kainic Acid; D017470:Receptors, Glutamate; D018092:Kainic Acid Receptors; D017981:Receptors, Neurotransmitter; D014316:Tritium