[3H]PN200-110 and [3H]glibenclamide binding in normal and cardiomyopathic hamsters.
1. We examined the binding of the Ca2+ channel ligand [3H]PN200-110 and the ATP-sensitive K+ channel ligand [3H]glibenclamide to brain and heart from cardiomyopathic hamsters and compared them to controls. 2. We found that [3H]PN200-110 binding site density was elevated in the heart, but not in the brain, of 30- and 180-day old cardiomyopathic hamsters when compared to controls. 3. [3H]Glibenclamide binding site density was greatly reduced in the heart of 180-day old cardiomyopathic animals compared with all other groups. 4. Quantitative autoradiography revealed that [3H]glibenclamide binding was elevated in several brain areas of 30-day old cardiomyopathic hamsters relative to controls. 5. It is concluded that alterations in both Ca2+ and K+ channels exist in the cardiomyopathic hamster.
Miller
J A
JA
Marion Merrell Dow Research Institute, Cincinnati, OH 45215.
Chmielewski
P A
PA
Rampe
D
D
D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D001345:Autoradiography; D001921:Brain / Q000378:metabolism; D015220:Calcium Channels / Q000187:drug effects; D006224:Cricetinae; D005905:Glyburide / Q000378:metabolism*; D006331:Heart Diseases / Q000378:metabolism*; D066298:In Vitro Techniques; D017275:Isradipine / Q000378:metabolism*; D008647:Mesocricetus; D009206:Myocardium / Q000378:metabolism; D015221:Potassium Channels / Q000187:drug effects
D015220:Calcium Channels; D015221:Potassium Channels; D000255:Adenosine Triphosphate; D005905:Glyburide; D017275:Isradipine