title: Guinea-pig ileum (GPI) and mouse vas deferens (MVD) preparations in the discovery, discrimination and parallel bioassay of opioid peptides.
abstract: As many as 47 amphibian and mammalian, natural and non-natural opioid peptides have been examined in guinea-pig ileum (GPI) and mouse vas deferens (MVD) preparations. The great value of these extremely simple and accessible tissue models in the identification, isolation and purification of endogenous opioid peptides, in studying structure/activity relationships, and in determining selectivity of the peptide molecules for the various opioid receptors, especially delta- and mu-receptors, is emphasized.
authors.affiliation: Institute of Medical Pharmacology III, University of Rome La Sapienza, Italy.
authors.forename: G F
authors.initials: GF
authors.lastname: Erspamer
authors.forename: C
authors.initials: C
authors.lastname: Severini
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001681:Biological Assay; D002364:Caseins; D004723:Endorphins / Q000302:isolation & purification / Q000494:pharmacology*; D004745:Enkephalins / Q000302:isolation & purification / Q000494:pharmacology; D006168:Guinea Pigs; D007082:Ileum; D007537:Isometric Contraction / Q000187:drug effects*; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D009130:Muscle, Smooth / Q000187:drug effects*; D009842:Oligopeptides / Q000302:isolation & purification / Q000494:pharmacology; D018847:Opioid Peptides; D017465:Receptors, Opioid, delta / Q000378:metabolism; D017450:Receptors, Opioid, mu / Q000378:metabolism; D013329:Structure-Activity Relationship; D014649:Vas Deferens
chemical_list: D002364:Caseins; D004723:Endorphins; D004745:Enkephalins; D009842:Oligopeptides; D018847:Opioid Peptides; D017465:Receptors, Opioid, delta; D017450:Receptors, Opioid, mu; C059567:deltorphin; C030058:dermorphin; C021968:beta-casomorphins