[Electrophoretic analysis of RNA from rotavirus strains isolated from children in the years 1986-1990].
Double stranded RNA of rotaviruses is composed of 11 segments which in standard and field strains may differ in electrophoretic mobility in polyacrylamide gel electrophoresis. This study was aimed at determination of electrophoretic differentiation of rotavirus strains found in samples of feces of children treated because of diarrhoea in two hospitals in Warsaw and in Kraków region in the years 1986-1990. Out of 85 positive samples from Warsaw and 90 from Krakow region, for investigation 44 and 36 strains, respectively were included. Totally for 71 strains well stained 11 segments of RNA were obtained which permitted for determination of their electrophoretic-types. Majority of strains, 60 (84%) were characterized by a long electrophoretic-type, whereas 11 (16%) had a short electrophoretic-type. Considering differences in migration speed of 1-4 and 7-9 segments, within long and short types, eight subtypes denominated as a-h were present. Subtypes a, c and d constituted 73% of all tested strains. These studies indicate that in Poland occur at least eight electrophoretic subtypes of rotaviruses and that this feature is partly correlated with belonging of strains to subgroups and antigenic serotypes.
Szczepaniak
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Zakład Wirusologii PZH w Warszawie.
Gut
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Jarzabek
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D002675:Child, Preschool; D003967:Diarrhea / Q000382:microbiology*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D012367:RNA, Viral / Q000032:analysis*; D012401:Rotavirus / Q000235:genetics*; D013045:Species Specificity
D012367:RNA, Viral
Elektroforetyczna analiza RNA szczepów rotawirusów izolowanych od dzieci w latach 1986-1990.