A comparison of three heparin-binding serine proteinase inhibitors.
The purpose of this study was to compare three heparin-binding plasma proteinase inhibitors in order to identify common and unique features of heparin binding and heparin-enhanced proteinase inhibition. Experiments with antithrombin, heparin cofactor, and protein C inhibitor were performed under identical conditions in order to facilitate comparisons. Synthetic peptides corresponding to the putative heparin binding regions of antithrombin, heparin cofactor, and protein C inhibitor bound to heparin directly and interfered in heparin-enhanced proteinase inhibition assays. All three inhibitors obeyed a ternary complex mechanism for heparin-enhanced thrombin inhibition, and the optimum heparin concentration was related to the apparent heparin affinity of the inhibitor. The maximum inhibition rate and rate enhancement due to heparin appeared to be unique properties of each inhibitor. In assays with heparin oligosaccharides of known size, only the antithrombin-thrombin reaction exhibited a sharp threshold for rate enhancement at 14-16 saccharide units. Acceleration of antithrombin inhibition of factor Xa, heparin cofactor inhibition of thrombin, and protein C inhibitor inhibition of thrombin, activated protein C, and factor Xa did not require a minimum saccharide size. The differences in heparin size dependence and rate enhancement of proteinase inhibition by these inhibitors might reflect differences in the importance of the ternary complex mechanism and other mechanisms, alterations in inhibitor reactivity, and orientation effects in heparin-enhanced proteinase inhibition.
Pratt
C W
CW
Department of Pathology, University of North Carolina School of Medicine, Chapel Hill 27599.
Whinna
H C
HC
Church
F C
FC
D000595:Amino Acid Sequence; D000990:Antithrombin III / Q000037:antagonists & inhibitors; D001665:Binding Sites; D001798:Blood Proteins / Q000378:metabolism; D002846:Chromatography, Affinity; D065427:Factor Xa Inhibitors; D006493:Heparin / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D008958:Models, Molecular; D008969:Molecular Sequence Data; D018046:Protein C Inhibitor; D015842:Serine Proteinase Inhibitors / Q000378:metabolism*; D013917:Thrombin / Q000037:antagonists & inhibitors
D001798:Blood Proteins; D065427:Factor Xa Inhibitors; D018046:Protein C Inhibitor; D015842:Serine Proteinase Inhibitors; D000990:Antithrombin III; D006493:Heparin; D013917:Thrombin