[Treatment of experimental liver tumors by in vivo suicide gene transfer in rats].
We investigated whether the transduction of a suicide gene might induce the elimination of malignant solid tumours. BDIX male rats were given an intra-hepatic injection of a mixture containing DHDK12 tumor cells and xenogeneic fibroblasts. The latter were producing either the herpes simplex virus type 1 thymidine kinase HSV1-TK- or nls-lac Z(control)-expressing recombinant retroviral particles. 5 days later, a time at which the tumor is macroscopic, all the rats were treated with ganciclovir, a nucleoside analog that is metabolized by HSV1-TK into a toxic compound. After 5 days of treatment, a dramatic reduction in tumour volume was noted in the HSV1-TK group. These results delineate a new therapeutical strategy for the treatment of disseminated liver metastases or of a large variety of solid malignant tumours.
Panis
Y
Y
Laboratoire de Biologie et Génétique des Pathologies immunitaires, Centre national de la Recherche scientifique, U.R.A. n. 1463, C.E.R.V.I., Hôpital de la Pitié-Salpêtrière, Paris.
Caruso
M
M
Houssin
D
D
Andreoletti
M
M
Khayat
D
D
Salzmann
J L
JL
Klatzmann
D
D
D000818:Animals; D016923:Cell Death / Q000187:drug effects; D005347:Fibroblasts / Q000187:drug effects / Q000201:enzymology; D015774:Ganciclovir / Q000494:pharmacology / Q000627:therapeutic use; D008114:Liver Neoplasms, Experimental / Q000188:drug therapy / Q000628:therapy*; D008297:Male; D051381:Rats; D018139:Simplexvirus / Q000235:genetics; D013937:Thymidine Kinase / Q000235:genetics; D014162:Transfection; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000201:enzymology
D013937:Thymidine Kinase; D015774:Ganciclovir
Traitement de tumeurs hépatiques expérimentales par transfert de gène suicide in vivo chez le rat.