Dipyridamole inhibits platelet aggregation induced by oxygen-derived free radicals.
Pyrogallol (a generator of superoxide anions) caused 50% increase in platelet aggregation induced by 400 microM of arachidonic acid. Dipyridamole did not produce a statistically significant inhibition of arachidonic-acid induced platelet aggregation, but it caused 100% inhibition of pyrogallol-stimulated platelet aggregation. Ferrous salts (Fe2+) induced 34% platelet aggregation which was inhibited (79.6%) by a concentration of dipyridamole of 10 microM. Dipyridamole inhibited ferrous-induced lipid peroxidation with IC-50 values of 17.5 microM. When arachidonic acid was used as aggregating agent, the corresponding IC-50 value was 140.5 microM. These results indicate that dipyridamole prevented platelet activation induced by oxygen-derived free radicals.
De la Cruz
J P
JP
Department of Pharmacology and Therapeutics, School of Medicine, University of Málaga, Spain.
García
P J
PJ
Sánchez de la Cuesta
F
F
D000328:Adult; D016718:Arachidonic Acid / Q000037:antagonists & inhibitors / Q000494:pharmacology; D001205:Ascorbic Acid / Q000037:antagonists & inhibitors / Q000494:pharmacology; D004176:Dipyridamole / Q000494:pharmacology*; D005296:Ferrous Compounds / Q000494:pharmacology; D005609:Free Radicals; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D010974:Platelet Aggregation / Q000187:drug effects*; D011748:Pyrogallol / Q000494:pharmacology*; D013481:Superoxides / Q000037:antagonists & inhibitors*
D005296:Ferrous Compounds; D005609:Free Radicals; D011748:Pyrogallol; D013481:Superoxides; D016718:Arachidonic Acid; C020748:ferrous sulfate; D004176:Dipyridamole; D001205:Ascorbic Acid