Rapid isolation of serum albumin using microporous PVC/silica cartridges.
A protocol has been developed for rapid isolation of albumin from rabbit or bovine serum. It is based on the use of microporous plastic silica sheets that have been fabricated into rapid flow-through devices called ACTI-MOD cartridges. In the first step, the untreated silica plastic matrix binds a major portion of the non-albumin protein. A second step, using an ACTI-MOD anion-exchange quaternary amine, further purifies and concentrates the albumin. At this stage, the albumin appears to be at least ninety-nine per cent pure based on sodium dodecyl sulfate gel electrophoresis.
Nochumson
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FMC Bio-Support Materials, Pine Brook, NJ 07058.
D000818:Animals; D001709:Biotechnology; D002417:Cattle; D002846:Chromatography, Affinity / Q000295:instrumentation* / Q000379:methods; D002852:Chromatography, Ion Exchange / Q000295:instrumentation / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel; D005069:Evaluation Studies as Topic; D011143:Polyvinyl Chloride; D011817:Rabbits; D012709:Serum Albumin / Q000302:isolation & purification*; D012822:Silicon Dioxide
D012709:Serum Albumin; D012822:Silicon Dioxide; D011143:Polyvinyl Chloride