Enzymatic synthesis of PAPS with an ATP-regeneration system.
Sulfate activating enzymes, ATP sulfurylase and APS kinase, were newly isolated from a thermophile, Bacillus stearothermophilus. Adenosine 3'-phosphate 5'-phosphosulfate (PAPS) was synthesized by these enzymes. The reaction proceeded more efficiently when an ATP-regeneration system, using acetate kinase, was coupled to the reaction system.
Ibuki
H
H
Biochemistry Department, Unitika Research and Development Center, Kyoto, Japan.
Tashiro
T
T
Hayashi
M
M
Nakajima
H
H
Liu
M C
MC
Suiko
M
M
D000084:Acetate Kinase / Q000737:chemistry; D000255:Adenosine Triphosphate / Q000737:chemistry*; D001411:Geobacillus stearothermophilus / Q000201:enzymology; D010724:Phosphoadenosine Phosphosulfate / Q000138:chemical synthesis* / Q000302:isolation & purification; D010770:Phosphotransferases / Q000737:chemistry; D017853:Phosphotransferases (Alcohol Group Acceptor); D013430:Sulfate Adenylyltransferase / Q000737:chemistry
D010724:Phosphoadenosine Phosphosulfate; D000255:Adenosine Triphosphate; D010770:Phosphotransferases; D017853:Phosphotransferases (Alcohol Group Acceptor); C019617:adenylylsulfate kinase; D000084:Acetate Kinase; D013430:Sulfate Adenylyltransferase