title: Erythrocyte membrane lipids and cationic transport systems in men.
abstract: OBJECTIVE
  The relationship between erythrocyte membrane and plasma lipids and various transmembrane erythrocyte cationic fluxes was examined in 53 normal men.
  
  
  METHODS
  Different measurements of erythrocyte transport systems were obtained: Na(+)-Li+ countertransport activity; Na+, K+ cotransport activity; Na+, K(+)-ATPase pump activity and the ground membrane permeability for Na+ and K+ as well as the intra-erythrocyte Na+, K+ and Mg2+ concentrations. Plasma cholesterol, triglycerides, phospholipids, free fatty acids, low- and high-density lipoprotein cholesterol levels and the erythrocyte membrane contents of cholesterol, phospholipids and free fatty acids were obtained from fasting subjects.
  
  
  RESULTS
  In single regression analysis the erythrocyte Na(+)-Li+ countertransport and Na+, K+ cotransport activities were negatively related to the erythrocyte membrane cholesterol, phospholipids and free fatty acids contents. The Na+, K(+)-ATPase pump activity as assessed by the ouabain-sensitive Na+ efflux was also inversely related to the membrane cholesterol and phospholipids contents. In multiple regression analysis the red blood cell Na(+)-Li+ countertransport activity was independently and negatively related to the membrane cholesterol and free fatty acids contents.
  
  
  CONCLUSIONS
  Our data show that an elevated level of erythrocyte membrane lipids in normal men is accompanied by lower Na(+)-Li+ countertransport, Na+, K+ cotransport and Na+, K(+)-ATPase pump activities.
authors.affiliation: Department of Pathophysiology, University of Leuven, Belgium.
authors.forename: P
authors.initials: P
authors.lastname: Lijnen
authors.forename: R
authors.initials: R
authors.lastname: Fagard
authors.forename: J
authors.initials: J
authors.lastname: Staessen
authors.forename: L
authors.initials: L
authors.lastname: Thijs
authors.forename: A
authors.initials: A
authors.lastname: Amery
mesh_terms: D000328:Adult; D004910:Erythrocyte Membrane / Q000737:chemistry* / Q000378:metabolism; D004912:Erythrocytes / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D016623:Ion Pumps / Q000502:physiology*; D017136:Ion Transport / Q000502:physiology; D008055:Lipids / Q000032:analysis* / Q000097:blood; D008094:Lithium / Q000378:metabolism*; D008274:Magnesium / Q000032:analysis; D008297:Male; D011188:Potassium / Q000032:analysis; D012044:Regression Analysis; D012964:Sodium / Q000032:analysis; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*
chemical_list: D016623:Ion Pumps; D008055:Lipids; D008094:Lithium; D012964:Sodium; D000254:Sodium-Potassium-Exchanging ATPase; D008274:Magnesium; D011188:Potassium