Morphological characteristics of a transplantable nephroblastoma (NB-Y) in F344 rats and the relation of tumour growth to hyper-reninaemia in NB-Y-bearing rats.
A transplantable tumour, designated NB-Y, was established from a spontaneous nephroblastoma in an F344 rat. NB-Y was serially passaged in syngeneic rats by subcutaneous implantation up to the 49th generation. The transplants grew into nodules with an average diameter of 5 cm and average weight of 92.9 g 4 weeks after implantation. The primary tumour and NB-Y consisted mainly of sheets or clusters of undifferentiated blastemal cells, which reacted immunohistochemically for vimentin but not for keratin. Renin-containing cells were observed in the small blood vessel walls within the primary tumour, but neoplastic cells of both primary tumour and NB-Y failed to stain for renin. Plasma renin activity was significantly higher (40.7 ng per ml per h) in transplanted rats 4 weeks after implantation compared with non-transplanted controls (28.0 ng per ml per h). Hyperplastic juxtaglomerular cells were often observed in rats bearing NB-Y. Sinusoidal dilatation was present in the liver, adrenal glands, pituitary gland and bone marrow of recipients, suggesting abnormal blood flow provoked via the renin-angiotensin system. The present study revealed the development of hyper-reninaemia in NB-Y-bearing rats, but its pathogenesis remains unknown.
Yamate
J
J
Nippon Institute for Biological Science, Tokyo, Japan.
Tajima
M
M
Shibuya
K
K
Saitoh
T
T
Kizaki
H
H
D000740:Anemia / Q000209:etiology; D000818:Animals; D005260:Female; D007678:Kidney Glomerulus / Q000473:pathology; D007680:Kidney Neoplasms / Q000097:blood / Q000150:complications / Q000473:pathology*; D008297:Male; D009363:Neoplasm Proteins / Q000097:blood*; D009368:Neoplasm Transplantation; D051381:Rats; D011916:Rats, Inbred F344; D012083:Renin / Q000097:blood*; D013047:Specific Pathogen-Free Organisms; D009396:Wilms Tumor / Q000097:blood / Q000150:complications / Q000473:pathology*
D009363:Neoplasm Proteins; D012083:Renin