Hydrogen peroxide-induced DNA damages in vitro revealed by electron microscopy.
A plasmid pSV2neo (5.6 kb) which is a recombinant SV40-based vector was subjected to hydrogen peroxide treatment in presence of iron in vitro (Fenton reaction). Transmission electron microscopy revealed that H2O2 through the Fenton reaction introduced different types of damages in DNA. Besides the expected nicked circular and linear molecules, single-stranded loops and plasmid multimers could be characterized. These observations on DNA damages might be helpful for better understanding of the effect of hydrogen peroxide on living cells in vivo.
Chaudhuri
S
S
Crystallography and Molecular Biology Division, Saha Institute of Nuclear Physics, Calcutta, India.
Bhattacharyya
N P
NP
Bhattacharjee
S B
SB
D004249:DNA Damage; D004274:DNA, Recombinant / Q000187:drug effects* / Q000648:ultrastructure; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D007501:Iron / Q000494:pharmacology; D008854:Microscopy, Electron; D010957:Plasmids; D013539:Simian virus 40 / Q000235:genetics
D004274:DNA, Recombinant; D006861:Hydrogen Peroxide; D007501:Iron