Asparagine-linked sugar chains of plasma membrane glycoproteins from healthy and common variable immunodeficiency B lymphoblastoid cell lines.
Asparagine-linked sugar chains of plasma membrane glycoproteins, which are formed by glycosylation during B cell maturation, were examined with B lymphoblastoid cell lines (LCLs) transformed by Epstein-Barr virus derived from healthy controls and patients with common variable immunodeficiency (CVI). Both two patients with CVI showed hypogammaglobulinemia and impaired B cell functions. LCLs from healthy controls and the patients showed CD19+ and HLA/DR+ in the cell surface and secreted IgM. In both healthy controls and the patients, the main oligosaccharide in asparagine-linked sugar chains of the membrane glycoproteins of LCLs was biantennary sugar chain with bisected GlcNAc (Gal2-GlcNAc2-Man3-GlcNAc-GlcNAc-Fuc-GlcNAcOT). Biantennary sugar chain with an alpha-fucosyl residue linked at the proximal GlcNAc was seen but biantennary sugar chain without an alpha-fucosyl residue at the proximal GlcNAc was little detected in each LCL. There was no difference in quality and quantity of asparagine-linked sugar chains between healthy controls and the patients. These results suggest that glycosylation during B cell maturation may not be impaired in patients with CVI.
Kondo
N
N
Department of Pediatrics, Gifu University School of Medicine, Japan.
Motoyoshi
F
F
Orii
T
T
D001216:Asparagine / Q000032:analysis*; D001402:B-Lymphocytes / Q000737:chemistry* / Q000473:pathology; D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000032:analysis*; D002462:Cell Membrane / Q000737:chemistry; D002472:Cell Transformation, Viral / Q000502:physiology; D002648:Child; D004560:Electricity; D004590:Electrophoresis, Paper / Q000379:methods; D005260:Female; D004854:Herpesvirus 4, Human / Q000502:physiology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007075:Immunoglobulin M / Q000032:analysis; D007153:Immunologic Deficiency Syndromes / Q000473:pathology*; D008297:Male; D008562:Membrane Glycoproteins / Q000032:analysis*; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000032:analysis
D002241:Carbohydrates; D007075:Immunoglobulin M; D008562:Membrane Glycoproteins; D009844:Oligosaccharides; D001216:Asparagine