Monoclonal and polyclonal antibodies for immunohistochemical detection of bovine parainfluenza type 3 virus in frozen and formalin-fixed paraffin-embedded tissues.
Accurate identification of bovine parainfluenza type 3 virus in bovine respiratory disease requires dependable, sensitive, and specific techniques for detection in affected animals. Immunohistochemical testing can be a rapid and reliable means of demonstration of virus in tissues from suspect cases; however, this procedure is dependent upon the quality of the antisera directed against the viral antigens. The production of rabbit polyclonal and murine monoclonal antibodies directed against bovine parainfluenza type 3 virus and techniques for their use in fresh-frozen and formalin-fixed paraffin-embedded tissues in immunofluorescence and immunoperoxidase-based immunohistochemical tests are described.
Haines
D M
DM
Department of Veterinary Microbiology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Canada.
Kendall
J C
JC
Remenda
B W
BW
Breker-Klassen
M M
MM
Clark
E G
EG
D000818:Animals; D000911:Antibodies, Monoclonal; D000956:Antigens, Viral / Q000032:analysis*; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology*; D015925:Cryopreservation / Q000662:veterinary; D005557:Formaldehyde; D007150:Immunohistochemistry / Q000379:methods*; D016612:Paraffin Embedding / Q000662:veterinary; D010224:Parainfluenza Virus 3, Human / Q000276:immunology / Q000302:isolation & purification*; D018184:Paramyxoviridae Infections / Q000382:microbiology / Q000662:veterinary*; D012141:Respiratory Tract Infections / Q000382:microbiology / Q000662:veterinary*; D016707:Tissue Fixation / Q000662:veterinary
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D005557:Formaldehyde