Rapid agonist-induced beta-adrenergic receptor kinase translocation in C6 glioma cells.
Exposure of C6 glioma cells to 1 microM isoproterenol leads to fast desensitization of the beta-adrenergic receptor/adenylyl cyclase system and transient receptor sequestration. It also triggers a very rapid and transient translocation to the plasma membrane of beta-adrenergic receptor kinase (beta ARK), a specific cytoplasmic kinase that phosphorylates only the agonist-occupied form of several G protein-coupled receptors. beta ARK-mediated receptor phosphorylation appears to be a suitable mechanism for the rapid regulation of adrenergic receptor function in the nervous tissue.
García-Higuera
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Departamento de Biología Molecular, Centro de Biología Molecular (CSIC-UAM), Universidad Autónoma, Madrid, Spain.
Mayor
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D000262:Adenylyl Cyclases / Q000378:metabolism*; D000818:Animals; D001692:Biological Transport; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism*; D005910:Glioma; D007700:Kinetics; D051381:Rats; D011943:Receptors, Adrenergic, beta / Q000378:metabolism*; D012995:Solubility; D014407:Tumor Cells, Cultured; D051552:beta-Adrenergic Receptor Kinases
D011943:Receptors, Adrenergic, beta; D017868:Cyclic AMP-Dependent Protein Kinases; D051552:beta-Adrenergic Receptor Kinases; D000262:Adenylyl Cyclases