Effects of cyclic nucleotide analogs on intraocular pressure and trauma-induced inflammation in the rabbit eye.
In this study the effects of cell-permeable 8-bromo-cAMP and 8-bromo-cGMP on intraocular pressure (IOP) and puncture-induced inflammatory response were investigated. Both 8-bromo-cAMP and 8-Bromo-cGMP reduced IOP when given subconjunctivally, but not topically. Subconjunctival administration of 8-bromo-cAMP induced a moderate disruption of the blood-aqueous barrier (BAB); in addition, subconjunctival 8-bromo-cAMP, but not topical 8-bromo-cAMP or subconjunctival 8-bromo-cGMP, reduced the disruption of the BAB and elevation of the aqueous PGE2 level after puncture trauma. It is concluded that the effects of 8-bromo-cAMP depend on the mode of administration, since this determines the concentration of 8-bromo-cAMP reached in the aqueous humor. It is suggested that 8-bromo-cAMP can partially suppress a slight inflammatory response by interference with the release of arachidonic acid from the tissues surrounding the aqueous humor.
Busch
M J
MJ
Department of Experimental Ophthalmology, The Netherlands Ophthalmic Research Institute, Amsterdam.
van Oosterhout
E J
EJ
Hoyng
P F
PF
D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000493:pharmacokinetics / Q000494:pharmacology*; D000818:Animals; D001082:Aqueous Humor / Q000378:metabolism*; D001693:Biological Transport, Active; D003228:Conjunctiva; D065306:Corneal Injuries; D000242:Cyclic AMP / Q000378:metabolism; D006152:Cyclic GMP / Q000031:analogs & derivatives* / Q000493:pharmacokinetics / Q000494:pharmacology; D015232:Dinoprostone / Q000378:metabolism; D005128:Eye Diseases / Q000209:etiology / Q000378:metabolism*; D005136:Eye Proteins / Q000378:metabolism; D007249:Inflammation / Q000378:metabolism; D007429:Intraocular Pressure / Q000187:drug effects*; D011817:Rabbits
D005136:Eye Proteins; D015124:8-Bromo Cyclic Adenosine Monophosphate; C016276:8-bromocyclic GMP; D000242:Cyclic AMP; D006152:Cyclic GMP; D015232:Dinoprostone