The role of free radicals in brassica-induced anaemia of sheep: an ESR spin trapping study.
The formation of reactive free radical species in sheep erythrocytes challenged with dimethyldisulphide, a brassica-derived haemolysin, has been investigated by electron spin resonance spectroscopy using the spin trap alpha-(4-pyridyl 1-oxide)-N-tert-butylnitrone. Erythrocytes exposed to this agent undergo a burst of free radical activity as demonstrated by the appearance of a spin adduct. The results suggest that haemolytic anaemia which can occur in sheep grazing forage brassicas is a consequence of oxidative stress.
McPhail
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DB
Macaulay Land Use Research Institute, Craigiebuckler, Aberdeen, Scotland.
Sibbald
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AM
D000743:Anemia, Hemolytic / Q000139:chemically induced / Q000503:physiopathology / Q000662:veterinary*; D000818:Animals; D001937:Brassica / Q000009:adverse effects*; D004121:Dimethyl Sulfoxide; D004578:Electron Spin Resonance Spectroscopy; D005260:Female; D005609:Free Radicals; D007263:Infusions, Parenteral; D012417:Rumen; D012756:Sheep; D012757:Sheep Diseases / Q000139:chemically induced / Q000503:physiopathology*
D005609:Free Radicals; D004121:Dimethyl Sulfoxide