title: Site of palmitoylation of a phospholipase C-resistant glycosylphosphatidylinositol membrane anchor.
abstract: The site of palmitoylation of the phosphatidylinositol moiety of the glycosyl-phosphatidylinositol membrane anchor of Trypanosoma brucei procyclic acidic repetitive protein was studied by using periodate oxidation. Analysis of the products by g.c.-m.s. allowed the assignment of 40 and 60% of the palmitate to the 2-position and the 3-position respectively of the myo-inositol ring.
authors.affiliation: Department of Biochemistry, University of Dundee, Scotland, U.K.
authors.forename: M A
authors.initials: MA
authors.lastname: Ferguson
mesh_terms: D000818:Animals; D002240:Carbohydrate Sequence; D008401:Gas Chromatography-Mass Spectrometry; D006017:Glycolipids / Q000378:metabolism*; D017261:Glycosylphosphatidylinositols; D008562:Membrane Glycoproteins; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D010168:Palmitates / Q000378:metabolism*; D010504:Periodic Acid / Q000737:chemistry; D043265:Phosphatidylinositol Diacylglycerol-Lyase; D010716:Phosphatidylinositols / Q000378:metabolism*; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism*; D015800:Protozoan Proteins / Q000378:metabolism*; D014346:Trypanosoma brucei brucei / Q000378:metabolism; D010738:Type C Phospholipases / Q000378:metabolism*; D014643:Variant Surface Glycoproteins, Trypanosoma / Q000378:metabolism
chemical_list: D006017:Glycolipids; D017261:Glycosylphosphatidylinositols; D008562:Membrane Glycoproteins; D010168:Palmitates; D010716:Phosphatidylinositols; D015800:Protozoan Proteins; D014643:Variant Surface Glycoproteins, Trypanosoma; C053277:procyclic acidic repetitive protein, Trypanosoma; D010504:Periodic Acid; D010727:Phosphoric Diester Hydrolases; D010738:Type C Phospholipases; D043265:Phosphatidylinositol Diacylglycerol-Lyase