Cloning and expression of a cDNA encoding the Epstein-Barr virus thymidine kinase gene.
A clone of the Epstein-Barr virus (EBV) thymidine kinase (TK) gene was derived from a cDNA library of P3HR1 cells. The gene product was expressed as a fusion protein in a procaryotic system by using T7 RNA polymerase. The recombinant TK showed a molecular mass of 67 kDa and was biologically active. Antiserum raised in mice immunized with partially purified TK recognized an antigen present in EBV-superinfected Raji cells using an indirect immunofluorescence assay.
Liu
M Y
MY
Graduate Institute of Microbiology, College of Medicine, National Taiwan University, Taipei, ROC.
Pai
C Y
CY
Shieh
S M
SM
Hsu
T Y
TY
Chen
J Y
JY
Yang
C S
CS
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics*; D015870:Gene Expression; D015723:Gene Library; D005814:Genes, Viral; D004854:Herpesvirus 4, Human / Q000201:enzymology / Q000235:genetics*; D007106:Immune Sera; D051379:Mice; D008969:Molecular Sequence Data; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D013937:Thymidine Kinase / Q000737:chemistry / Q000235:genetics* / Q000276:immunology / Q000378:metabolism
D004279:DNA, Viral; D007106:Immune Sera; D011993:Recombinant Fusion Proteins; D004247:DNA; D013937:Thymidine Kinase