PPADS, a novel functionally selective antagonist of P2 purinoceptor-mediated responses.
We have characterized PPADS (pyridoxalphosphate-6-azophenyl-2',4'-disulfonic acid) as a novel antagonist which selectively blocks P2 purinoceptor-mediated responses in rabbit vas deferens at pre- and postjunctional sites. PPADS did not interact with alpha 1-adrenoceptors, muscarinic M2 and M3 receptors, histamine H1 and adenosine A1 receptors. Thus, PPADS is a novel and useful pharmacological tool to study co-transmission in tissues where ATP and co-existing neurotransmitters act in concert.
Lambrecht
G
G
Department of Pharmacology, University of Frankfurt, Germany.
Friebe
T
T
Grimm
U
U
Windscheif
U
U
Bungardt
E
E
Hildebrandt
C
C
Bäumert
H G
HG
Spatz-Kümbel
G
G
Mutschler
E
E
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D004558:Electric Stimulation; D008297:Male; D011224:Prazosin / Q000494:pharmacology; D058914:Purinergic Antagonists; D011732:Pyridoxal Phosphate / Q000031:analogs & derivatives* / Q000494:pharmacology; D011817:Rabbits; D009435:Synaptic Transmission / Q000187:drug effects*; D014649:Vas Deferens / Q000187:drug effects / Q000294:innervation
D058914:Purinergic Antagonists; C077792:pyridoxal phosphate-6-azophenyl-2',4'-disulfonic acid; D011732:Pyridoxal Phosphate; D000255:Adenosine Triphosphate; D011224:Prazosin