Stabilities of hydroxyl radical spin adducts of PBN-type spin traps.
The stability of the hydroxyl spin adduct of nine different PBN-type spin traps has been examined in phosphate buffer solutions of various pH. The hydroxyl adduct is produced by short illumination of hydrogen peroxide with UV light in the presence of spin trap and the decay of its EPR signal followed. The stability measured by the half life of the first-order decay is strongly dependent on the pH of the solution and the structure of the aromatic ring used in the trap. All hydroxyl adducts are more stable in acidic media. tert-Butyl hydroaminoxyl is detected as a degradation product of the hydroxyl adduct from all spin traps.
Janzen
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EG
National Biomedical Center for Spin Trapping and Free Radicals, Oklahoma Medical Research Foundation, Oklahoma City 73104.
Kotake
Y
Y
Hinton
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RD
D003497:Cyclic N-Oxides; D004355:Drug Stability; D005609:Free Radicals; D006863:Hydrogen-Ion Concentration; D006878:Hydroxides / Q000737:chemistry*; D017665:Hydroxyl Radical; D007700:Kinetics; D015394:Molecular Structure; D009589:Nitrogen Oxides / Q000737:chemistry*; D013113:Spin Labels
D003497:Cyclic N-Oxides; D005609:Free Radicals; D006878:Hydroxides; D009589:Nitrogen Oxides; D013113:Spin Labels; D017665:Hydroxyl Radical; C029217:phenyl-N-tert-butylnitrone