title: Sensitive procedures for measuring chloride fluxes mediated by the purified glycine receptor incorporated into phospholipid vesicles.
abstract: Two novel methods have been developed to directly measure chloride influx into purified glycine receptor-containing phospholipid vesicles. Using a method based on the fluorescence quenching of a chloride-sensitive probe entrapped into the vesicles, a chloride influx was detected which could be enhanced by glycine and completely abolished by the antagonist strychnine. In addition, by tracing the 36Cl- influx into the proteoliposomes, a stimulatory effect of both glycine and beta-alanine could be seen, which can be inhibited by strychnine and other glycine antagonists. These data, together with a previous report demonstrating ligand-mediated iodide fluxes in the same preparation (Biochemistry, 28 (1989) 6405-6409), clearly demonstrate the utility of the reconstituted receptor preparation to investigate some ion channel and pharmacological properties of the glycine receptor.
authors.affiliation: Departamento de Biología Molecular UAM-CSIC, Facultad de Ciencias, Universidad Autónoma de Madrid, Spain.
authors.forename: M
authors.initials: M
authors.lastname: García-Calvo
authors.forename: F
authors.initials: F
authors.lastname: Valdivieso
authors.forename: F
authors.initials: F
authors.lastname: Mayor
authors.forename: J
authors.initials: J
authors.lastname: Vázquez
mesh_terms: D000818:Animals; D018118:Chloride Channels; D002712:Chlorides / Q000378:metabolism*; D005998:Glycine / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D015640:Ion Channel Gating / Q000187:drug effects; D008081:Liposomes; D008565:Membrane Proteins / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D018009:Receptors, Glycine; D017981:Receptors, Neurotransmitter / Q000187:drug effects / Q000378:metabolism*; D013331:Strychnine / Q000494:pharmacology; D015091:beta-Alanine / Q000494:pharmacology
chemical_list: D018118:Chloride Channels; D002712:Chlorides; D008081:Liposomes; D008565:Membrane Proteins; D018009:Receptors, Glycine; D017981:Receptors, Neurotransmitter; D015091:beta-Alanine; D013331:Strychnine; D005998:Glycine