Human T-cell leukemia virus-1-positive cell line established from a patient with small cell lung cancer.
A stable cell line, KHM-3S, was established from a patient with small cell lung cancer (SCLC), who had a high serum level of soluble interleukin 2 receptors (sIL2-R) and was seropositive for human T cell leukemia virus (HTLV)-1. KHM-3S cells were positive for IL2-R (Tac) and NKH-1, but negative for other lymphocytic markers such as OKT 11, OKT 4, OKT 8, T cell receptor (WT 31), B 1, and B 4. Moreover, the KHM-3S cells were negative for leukocyte common antigen and strongly positive for neuron-specific enolase (NSE). Secretion of sIL2-R and NSE by the KHM-3S line was detected by an enzyme-linked immunosorbent assay. Rearrangement of the T cell receptor gene and monoclonal HTLV-1 integration were found by Southern blot analysis of KHM-3S DNA. However, Northern blot analysis showed no T cell receptor mRNA. KHM-3S may be useful for studies on the role of HTLV-1 in carcinogenesis and IL2-R expression in SCLC.
Matsuzaki
H
H
Second Department of Internal Medicine, Kumamoto University Medical School.
Hata
H
H
Asou
N
N
Yoshida
M
M
Matsuno
F
F
Takeya
M
M
Yamaguchi
K
K
Sanada
I
I
Takatsuki
K
K
D000954:Antigens, Surface / Q000032:analysis; D015152:Blotting, Northern; D015139:Blotting, Southern; D018288:Carcinoma, Small Cell / Q000382:microbiology* / Q000473:pathology / Q000503:physiopathology; D015490:HTLV-I Infections / Q000097:blood / Q000473:pathology* / Q000503:physiopathology; D015368:Human T-lymphotropic virus 1 / Q000302:isolation & purification*; D006801:Humans; D007621:Karyotyping; D008175:Lung Neoplasms / Q000382:microbiology* / Q000473:pathology / Q000503:physiopathology; D008297:Male; D008875:Middle Aged; D015375:Receptors, Interleukin-2 / Q000096:biosynthesis; D014407:Tumor Cells, Cultured
D000954:Antigens, Surface; D015375:Receptors, Interleukin-2