Biochemical defects of outermost layer deficient mutants during sporulation of Bacillus megaterium.
To determine the regulation of morphogenesis of the outermost layer, the thick layer outside the inner coat, of the Bacillus megaterium spore, we isolated 15 outermost layer deficient mutants of B. megaterium using transposon Tn917. Three mutant strains lacked both synthesis of the 48-kDa outermost layer protein and induction of two initial enzymes for galactosamine-6-phosphate polymer synthesis, evidence that these biochemical events are regulated in the cascade system during morphogenesis of the outermost layer.
Takubo
Y
Y
Faculty of Pharmaceutical Sciences, Osaka University, Japan.
Inoue
T
T
Kunimatsu
T
T
Kondo
M
M
Nishihara
T
T
D001410:Bacillus megaterium / Q000235:genetics* / Q000502:physiology / Q000648:ultrastructure; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification; D004251:DNA Transposable Elements; D008854:Microscopy, Electron; D008970:Molecular Weight; D009154:Mutation; D011134:Polysaccharides / Q000096:biosynthesis; D013171:Spores, Bacterial / Q000502:physiology
D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D011134:Polysaccharides