Sequences within the feline immunodeficiency virus long terminal repeat that regulate gene expression and respond to activation by feline herpesvirus type 1.
We constructed a series of deletion mutants of feline immunodeficiency virus (FIV) long terminal repeat (LTR) to identify the regions that regulate gene expression and are responsive to trans-activation of FIV LTR by feline herpes-virus type 1 (FHV-1). We demonstrated that sequences between -124 and -79, and between -21 and -32 (relative to the cap site) are essential for gene expression of FIV in Felis catus whole fetus 4 (fcwf-4) cells. Further, we demonstrated that the sequence between -63 and -23 responds to trans-activation of FIV LTR by FHV-1 in fcwf-4 cells.
Kawaguchi
Y
Y
Department of Veterinary Microbiology, Faculty of Agriculture, University of Tokyo, Japan.
Norimine
J
J
Miyazawa
T
T
Kai
C
C
Mikami
T
T
D000818:Animals; D001483:Base Sequence; D002415:Cats; D002460:Cell Line; D004279:DNA, Viral; D015967:Gene Expression Regulation, Viral; D006564:Herpesviridae / Q000235:genetics*; D016089:Immunodeficiency Virus, Feline / Q000235:genetics*; D008969:Molecular Sequence Data; D016296:Mutagenesis; D012091:Repetitive Sequences, Nucleic Acid; D015533:Transcriptional Activation
D004279:DNA, Viral