Low density lipoprotein oxidation by stimulated neutrophils and ferritin.
Low density lipoprotein (LDL) oxidation mediated by phorbol myristate acetate (PMA)- and formylmethionylleucylphenylalanine (FMLP) -stimulated human neutrophils was enhanced by 70% in the presence of ferritin. Iron released from ferritin by the superoxide anion generated in the respiratory burst of stimulated neutrophils is shown to be involved in lipoprotein oxidation. Ascorbate (100 microM), superoxide dismutase (10 micrograms/ml) and uric acid (430 microM) showed inhibitory effects of 30% [corrected], 70% and 50% on LDL oxidation, respectively. Ceruloplasmin (2.7 microM) potentiated LDL oxidation by stimulated neutrophils and ferritin, both alone and in the presence of methionine. Methionine (1 mM) and catalase (30 micrograms/ml) increased LDL oxidation by stimulated neutrophils and ferritin. These data suggest that LDL oxidation by stimulated neutrophils and ferritin may be relevant in inflammation when both neutrophils and ferritin are increased.
Abdalla
D S
DS
Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, Brazil.
Campa
A
A
Monteiro
H P
HP
D001059:Apoproteins / Q000494:pharmacology; D001205:Ascorbic Acid / Q000494:pharmacology; D002374:Catalase / Q000494:pharmacology; D002570:Ceruloplasmin / Q000494:pharmacology; D005293:Ferritins / Q000494:pharmacology*; D006801:Humans; D015227:Lipid Peroxidation; D008077:Lipoproteins, LDL / Q000378:metabolism*; D008715:Methionine / Q000494:pharmacology; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism*; D010084:Oxidation-Reduction; D013481:Superoxides / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D017392:Thiobarbituric Acid Reactive Substances; D014168:Transferrin / Q000494:pharmacology; D014527:Uric Acid / Q000494:pharmacology
D001059:Apoproteins; D008077:Lipoproteins, LDL; D017392:Thiobarbituric Acid Reactive Substances; D014168:Transferrin; C028284:apotransferrin; D013481:Superoxides; D014527:Uric Acid; D009240:N-Formylmethionine Leucyl-Phenylalanine; D005293:Ferritins; D008715:Methionine; D002374:Catalase; D002570:Ceruloplasmin; D013755:Tetradecanoylphorbol Acetate; D001205:Ascorbic Acid