Enkephalin induces Ca2+ mobilization in single cells of bradykinin-sensitized differentiated neuroblastoma hybridoma (NG108-15) cells.
A study of the intracellular Ca2+ ([Ca2+]i) response of differentiated neuroblastoma x glioma hybrid cells (NG108-15 cell) to enkephalin (EK) was carried out by fura-2 video-imaging. EK alone did not influence [Ca2+]i in single cells. The opioid did, however, induce a marked [Ca2+]i rise, when the cells were incubated with bradykinin (BK) prior to the EK treatment. Such BK-assisted stimulation of the differentiated hybridoma cells by EK was completely abolished by pertussis toxin treatment. These results suggest that in single NG108-15 cells, EK induces Ca2+ mobilization which is assisted by cross-talk between the EK and BK receptor systems via a pertussis toxin-sensitive G protein.
Tomura
H
H
Department of Physical Biochemistry, Gunma University, Maebashi, Japan.
Okajima
F
F
Kondo
Y
Y
D000818:Animals; D001920:Bradykinin / Q000494:pharmacology*; D003994:Bucladesine / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism* / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects; D004533:Egtazic Acid / Q000494:pharmacology; D004745:Enkephalins / Q000494:pharmacology*; D016257:Fura-2; D005910:Glioma; D006822:Hybrid Cells; D007700:Kinetics; D051379:Mice; D008856:Microscopy, Fluorescence; D009447:Neuroblastoma; D037342:Pertussis Toxin; D051381:Rats; D013997:Time Factors; D014741:Video Recording; D010566:Virulence Factors, Bordetella / Q000494:pharmacology
D004745:Enkephalins; D010566:Virulence Factors, Bordetella; D004533:Egtazic Acid; D003994:Bucladesine; D037342:Pertussis Toxin; D001920:Bradykinin; D002118:Calcium; D016257:Fura-2