Plasmodium falciparum and Plasmodium chabaudi: characterization of glycosylphosphatidylinositol-degrading activities.
Merozoites of malaria parasites have a membrane-bound serine protease whose solubilization and subsequent activity depend on a parasite-derived glycosylphosphatidylinositol-phospholipase C (GPI-PLC). The GPI-degrading activities from both Plasmodium falciparum and Plasmodium chabaudi have been characterized and partially purified by phenylboronate chromatography. They are membrane-bound, developmentally regulated, calcium-independent enzymes and as such they resemble GPI-PLC of Trypanosoma brucei. Furthermore, a T. brucei GPI-PLC-specific monoclonal antibody (mAT3) immunoprecipitates the plasmodial GPI-degrading activity. Thin-layer chromatography is suggestive of two activities: a GPI-PLC and a phospholipase A.
Braun-Breton
C
C
Department of Immunology, Institut Pasteur, Paris, France.
Blisnick
T
T
Barbot
P
P
Bülow
R
R
Pereira da Silva
L
L
Langsley
G
G
D000818:Animals; D003429:Cross Reactions; D006017:Glycolipids / Q000378:metabolism*; D054800:Glycosylphosphatidylinositol Diacylglycerol-Lyase; D017261:Glycosylphosphatidylinositols; D043265:Phosphatidylinositol Diacylglycerol-Lyase; D010716:Phosphatidylinositols / Q000378:metabolism*; D010741:Phospholipases A / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D010727:Phosphoric Diester Hydrolases / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D016788:Plasmodium chabaudi / Q000201:enzymology*; D010963:Plasmodium falciparum / Q000201:enzymology*; D014346:Trypanosoma brucei brucei / Q000201:enzymology; D010738:Type C Phospholipases / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*
D006017:Glycolipids; D017261:Glycosylphosphatidylinositols; D010716:Phosphatidylinositols; D010741:Phospholipases A; D010727:Phosphoric Diester Hydrolases; D010738:Type C Phospholipases; D043265:Phosphatidylinositol Diacylglycerol-Lyase; D054800:Glycosylphosphatidylinositol Diacylglycerol-Lyase