Expression of active human interferon-beta in transgenic plants.
Tobacco plants were transformed with the human gene for interferon-beta (IFN-beta). Transformation was determined by the polymerase chain reaction (PCR), and expression was determined by Western blot analysis, by purifying the IFN from the transgenic plants, and by bioassays indicating its activity in human cells. Plants expressing IFN-beta were self-pollinated. IFN-beta-expressing progeny plants were selected and produced active IFN-beta, indicating stable transformation.
Edelbaum
O
O
Virus Laboratory, Hebrew University of Jerusalem, Faculty of Agriculture, Rehovot, Israel.
Stein
D
D
Holland
N
N
Gafni
Y
Y
Livneh
O
O
Novick
D
D
Rubinstein
M
M
Sela
I
I
D001483:Base Sequence; D015153:Blotting, Western; D002460:Cell Line; D002846:Chromatography, Affinity; D003588:Cytopathogenic Effect, Viral; D015870:Gene Expression; D006801:Humans; D016899:Interferon-beta / Q000235:genetics* / Q000302:isolation & purification / Q000494:pharmacology; D008969:Molecular Sequence Data; D030821:Plants, Genetically Modified / Q000737:chemistry / Q000235:genetics*; D010947:Plants, Toxic; D010957:Plasmids; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins; D014026:Nicotiana; D014162:Transfection; D014721:Vesicular stomatitis Indiana virus / Q000187:drug effects
D011994:Recombinant Proteins; D016899:Interferon-beta