Cryo-EM of the native structure of the calcium release channel/ryanodine receptor from sarcoplasmic reticulum.
The native structure of the calcium release channel (ryanodine receptor) from rabbit skeletal muscle has been analyzed in two dimensions from electron micrographs of frozen hydrated specimens. Within a resolution of 3.0 nm there is excellent agreement between the structure as seen in vitreous water and in negative stained specimens. Features seen in the three-dimensional reconstruction of the negatively stained channel can be identified in the projection of the unstained receptor.
Radermacher
M
M
Wadsworth Center for Laboratories and Research, New York State Department of Health, Albany 12201-0509.
Wagenknecht
T
T
Grassucci
R
R
Frank
J
J
Inui
M
M
Chadwick
C
C
Fleischer
S
S
D000818:Animals; D055592:Biophysical Phenomena; D001703:Biophysics; D015220:Calcium Channels / Q000648:ultrastructure*; D005615:Freezing; D008854:Microscopy, Electron; D009124:Muscle Proteins / Q000648:ultrastructure*; D011487:Protein Conformation; D011817:Rabbits; D011950:Receptors, Cholinergic / Q000648:ultrastructure*; D019837:Ryanodine Receptor Calcium Release Channel; D012519:Sarcoplasmic Reticulum / Q000648:ultrastructure
D015220:Calcium Channels; D009124:Muscle Proteins; D011950:Receptors, Cholinergic; D019837:Ryanodine Receptor Calcium Release Channel