title: Ovine mitochondrial DNA: restriction enzyme analysis, mapping and sequencing data.
abstract: Restriction endonuclease fragment patterns of mitochondrial DNA (mtDNA) in sheep were analysed with 11 enzymes. Four breeds (Merinolandschaf, Rhoenschaf, Schwarzkoepfiges Fleischschaf and Skudde) of domestic sheep and European Mouflon were examined. A restriction map with 28 cleavage sites of seven enzymes was established. KpnI and PstI do not cut ovine mtDNA. Two EcoRI fragments of Merinolandschaf, Rhoenschaf and Mouflon each were cloned and partially sequenced. Intraspecific nucleotide sequence differences within 1.101 kb ranged from 0.09 to 0.27%. Hybridization analysis with a fragment of porcine mtDNA along with sequencing data from cloned fragments was used for orientation of the restriction map along the bovine sequence. Ovine mtDNA sequences encompassing parts of the Cyt.b-, ND5-, CoIII- and ATPase6 genes were compared with the corresponding sequences of the bovine mtDNA.
authors.affiliation: Institut für Tierzucht und Haustiergenetik, Justus-Liebig Universität, Giessen, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Hiendleder
authors.forename: W
authors.initials: W
authors.lastname: Hecht
authors.forename: V
authors.initials: V
authors.lastname: Dzapo
authors.forename: R
authors.initials: R
authors.lastname: Wassmuth
mesh_terms: D000251:Adenosine Triphosphatases / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D001059:Apoproteins / Q000235:genetics; D001483:Base Sequence; D003573:Cytochrome b Group / Q000235:genetics; D045303:Cytochromes b; D004247:DNA; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004272:DNA, Mitochondrial / Q000235:genetics*; D014644:Genetic Variation; D008928:Mitochondria / Q000201:enzymology; D008969:Molecular Sequence Data; D009245:NADH Dehydrogenase / Q000235:genetics; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D012756:Sheep / Q000235:genetics*
chemical_list: D001059:Apoproteins; D003573:Cytochrome b Group; D004272:DNA, Mitochondrial; D004247:DNA; D045303:Cytochromes b; D009245:NADH Dehydrogenase; D004262:DNA Restriction Enzymes; D000251:Adenosine Triphosphatases