title: Cyclic AMP inhibition of lipopolysaccharide-induced restriction of Legionella pneumophila growth in macrophage cultures.
abstract: The mechanism of the effects of lipopolysaccharide (LPS) on macrophages in terms of replication of intracellular facultative bacteria is unclear. It was found in the present study that the anti-Legionella pneumophila activity induced by LPS in macrophages from susceptible A/J mice was reversed in vitro by dibutyryl cyclic AMP (DcAMP). A 24-h pretreatment of murine thioglycolate-elicited macrophages with LPS resulted in an enhanced ability of these cells to inhibit the intracellular growth of L. pneumophila. This anti-L. pneumophila activity of macrophages induced by LPS was inhibited when DcAMP (10(-3) to 10(-5) M) was present during preincubation with LPS. The addition of DcAMP to the cultures was more effective before LPS treatment than after treatment. The effect of DcAMP was dose dependent. The secretion and production of acid phosphatase by LPS-activated macrophages were also inhibited by the addition of DcAMP before LPS treatment. Furthermore, the anti-L. pneumophila activity of macrophages induced by LPS could also be reversed in vitro by treatment with prostaglandin E2, colchicine, isoproterenol, theophylline, or hydrocortisone, all of which are known to increase the intracellular levels of cyclic AMP in various tissues. These observations indicate that the anti-L. pneumophila activity induced by LPS treatment can be modified by mechanisms involving cyclic nucleotide metabolism.
authors.affiliation: Department of Medical Microbiology and Immunology, University of South Florida College of Medicine, Tampa 33612.
authors.forename: K
authors.initials: K
authors.lastname: Egawa
authors.forename: T W
authors.initials: TW
authors.lastname: Klein
authors.forename: Y
authors.initials: Y
authors.lastname: Yamamoto
authors.forename: C A
authors.initials: CA
authors.lastname: Newton
authors.forename: H
authors.initials: H
authors.lastname: Friedman
mesh_terms: D000818:Animals; D003994:Bucladesine / Q000494:pharmacology*; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000032:analysis; D015232:Dinoprostone / Q000494:pharmacology; D005260:Female; D016952:Legionella pneumophila / Q000187:drug effects* / Q000254:growth & development; D008070:Lipopolysaccharides; D008264:Macrophages / Q000382:microbiology*; D051379:Mice
chemical_list: D008070:Lipopolysaccharides; D003994:Bucladesine; D000242:Cyclic AMP; D015232:Dinoprostone