Examination of the buoyant density of hepatitis C virus by the polymerase chain reaction.
Sucrose and cesium chloride density gradients were used to fractionate hepatitis C virus (HCV) infectious chimpanzee plasma. The fractionated plasma was then evaluated for HCV RNA sequences using cDNA synthesis and the polymerase chain reaction (cDNA/PCR). cDNA/PCR detectable HCV RNA was identified repeatedly in two regions. One region was at the top of the gradients with a buoyant density of < or = 1.03 g/cm3, the other at a density of approximately 1.18-1.21 g/cm3.
Carrick
R J
RJ
Abbott Laboratories, North Chicago, IL 60064.
Schlauder
G G
GG
Peterson
D A
DA
Mushahwar
I K
IK
D000818:Animals; D001483:Base Sequence; D002499:Centrifugation, Density Gradient; D002586:Cesium; D002712:Chlorides; D016174:Hepacivirus / Q000737:chemistry* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D002679:Pan troglodytes; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000097:blood / Q000737:chemistry* / Q000302:isolation & purification; D013395:Sucrose
D002712:Chlorides; D012367:RNA, Viral; D002586:Cesium; D013395:Sucrose; C028019:cesium chloride