Lack of in vitro interference between SA-11 rotavirus and attenuated poliovirus type 1.
To determine if rotavirus interferes with the multiplication of poliovirus, and hence the efficiency of the polio vaccine, the effect of timing and concentration of Simian rotavirus (SSA-11) on polio-1 infection in MA-104 cells was studied in vitro by evaluating the cytopathic effect, the reduction of the infectivity titers and the visualization of viral particles by electron microscopy. We found that poliovirus 1 was able to replicate when the challenge dose was administered within the first 8 h following SA-11 infection and with titers ten times lower than those of rotavirus. Hence, non-interference effect was observed in this in vitro model.
Guiscafré Gallardo
J P
JP
Clinical Infectious Diseases Research Unit, Instituto Mexicano del Seguro Social, D.F.
Zamora Zavala
C
C
Alvarez y Muñoz
T
T
Muñoz
O
O
D000818:Animals; D002460:Cell Line; D003588:Cytopathogenic Effect, Viral; D007106:Immune Sera; D007668:Kidney; D008253:Macaca mulatta; D017955:Poliovirus / Q000276:immunology / Q000502:physiology* / Q000648:ultrastructure; D011055:Poliovirus Vaccine, Oral; D011817:Rabbits; D012401:Rotavirus / Q000276:immunology / Q000502:physiology* / Q000648:ultrastructure; D018139:Simplexvirus / Q000502:physiology; D014709:Vero Cells; D014762:Viral Interference; D014771:Virion / Q000648:ultrastructure; D014779:Virus Replication
D007106:Immune Sera; D011055:Poliovirus Vaccine, Oral