Partial structures and binding studies of maitotoxin, the most potent marine toxin.
Partial structures of maitotoxin (MTX) were deduced by extensive two-dimensional NMR measurements, showing that the toxin had carbon-carbon double bonds in both termini of the molecule, and a primary alcohol group at the end. These functionalities were used for preparation of a radioligand of MTX; [3H]H-MTX and [3H]benzoyl-MTX. Both radioligands had high levels of non-specific binding to tissues. The binding of [3H]MTX to rat glioma C6 cells was inhibited by a didesulfo-MTX, suggesting the presence of the specific site for MTX-binding on the external surface of the cell membrane.
Murata
M
M
Faculty of Agriculture, Tohoku University, Sendai, Japan.
Sasaki
M
M
Yokoyama
A
A
Iwashita
T
T
Gusovsky
F
F
Daly
J W
JW
Yasumoto
T
T
D000818:Animals; D006224:Cricetinae; D005910:Glioma / Q000378:metabolism; D007340:Insulinoma / Q000378:metabolism; D008024:Ligands; D009682:Magnetic Resonance Spectroscopy; D008387:Marine Toxins / Q000737:chemistry* / Q000378:metabolism*; D015394:Molecular Structure; D035964:Oxocins; D051381:Rats; D014316:Tritium; D014407:Tumor Cells, Cultured; D000095702:Polyether Toxins
D008024:Ligands; D008387:Marine Toxins; D035964:Oxocins; D014316:Tritium; C020394:maitotoxin; D000095702:Polyether Toxins