Cloning of the gene encoding a glycylprolyl aminopeptidase from Porphyromonas gingivalis.
A genomic library of Porphyromonas gingivalis 381 was constructed in the cosmid vector pHC79. A clone, pSN1, was identified by the expression of glycylprolyl-naphthylamide hydrolysing activity. The DNA insert contained within the cosmid pSN1 was subcloned into the plasmid vector pBR328 to create the recombinant plasmid pSN11 containing a 2.9 kb EcoRV insert. An Escherichia coli transformant containing pSN11 produced a protein having a molecular weight of 75 kDa. Southern-blot hybridization revealed that the 2.9 kb EcoRV DNA hybridized with an identical sized Eco RV DNA fragment in the chromosomal DNA of P. gingivalis 381.
Nakamura
S
S
Fundamental Research Laboratory, Sunstar Inc., Osaka, Japan.
Takeuchi
A
A
Masamoto
Y
Y
Abiko
Y
Y
Hayakawa
M
M
Takiguchi
H
H
D015139:Blotting, Southern; D003001:Cloning, Molecular / Q000379:methods; D003360:Cosmids; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D008970:Molecular Weight; D010510:Periodontal Diseases / Q000382:microbiology; D016966:Porphyromonas gingivalis / Q000201:enzymology* / Q000235:genetics*; D011994:Recombinant Proteins / Q000235:genetics; D015183:Restriction Mapping
D011994:Recombinant Proteins; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases; C059607:glycylprolyl dipeptidase