Protonation states of the catalytic intermediates of cytochrome c oxidase.
Protonation changes accompanying conversion of oxidised (O state) cytochrome c oxidase to the 2-electron-reduced P state, and 3-electron-reduced F state at pH 8.0 have been measured. It was found that 2 and 3 protons, respectively, were taken up. The fourth proton required for the reduction of O2 to H2O must therefore be consumed in the remaining F----O portion of the catalytic cycle.
Mitchell
R
R
Glynn Research Institute, Bodmin, Cornwall, UK.
Mitchell
P
P
Rich
P R
PR
D000818:Animals; D002384:Catalysis; D002417:Cattle; D003576:Electron Transport Complex IV / Q000737:chemistry / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D011522:Protons
D011522:Protons; D003576:Electron Transport Complex IV