Dexamethasone blocks nerve growth factor induction of nerve growth factor receptor mRNA in PC12 cells.
Glucocorticoids and nerve growth factor (NGF) have been shown to have antagonistic effects on chromaffin cells in vivo. Here we determined the effect of the synthetic glucocorticoid, dexamethasone, on levels of mRNA for the nerve growth factor receptor (NGFR) in rat PC12 pheochromocytoma cells. Following administration of dexamethasone (1 microM) there is a decline in NGFR mRNA expression. More importantly, administration of dexamethasone appears to block the NGF-mediated induction of NGFR when both agents are administered simultaneously. These data support the hypothesis that glucocorticoids and NGF act in opposition in determination of the phenotype of chromaffin cells.
Foreman
P J
PJ
Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch, Galveston 77550.
Taglialatela
G
G
Jackson
G R
GR
Perez-Polo
J R
JR
D000818:Animals; D015152:Blotting, Northern; D002454:Cell Differentiation / Q000187:drug effects; D003864:Depression, Chemical; D003907:Dexamethasone / Q000494:pharmacology*; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D009363:Neoplasm Proteins / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification; D009414:Nerve Growth Factors / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D016716:PC12 Cells / Q000187:drug effects*; D010641:Phenotype; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D011956:Receptors, Cell Surface / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification; D017475:Receptors, Nerve Growth Factor
D009363:Neoplasm Proteins; D009414:Nerve Growth Factors; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D017475:Receptors, Nerve Growth Factor; D003907:Dexamethasone