The virulence-determining genomic BamHI fragment 4 of pseudorabies virus contains genes corresponding to the UL15 (partial), UL18, UL19, UL20, and UL21 genes of herpes simplex virus and a putative origin of replication.
The genomic BamHI-fragment 4 of pseudorabies virus (PrV) has previously been shown to encode functions necessary for expression of PrV neurovirulence (B. Lomniczi, S. Watanabe, T. Ben-Porat, and A. S. Kaplan, 1984, J. Virol. 52, 198-205). To identify proteins that might be involved in the neurotropism of PrV we sequenced the complete 9382-bp fragment BamHI-4, the longest contiguous sequence determined in the UL region of PrV so far, and analyzed its coding capacity. In an arrangement similar to that found in herpes simplex virus type 1 we identified complete open reading frames encoding proteins with strong homology to the UL18 (50% homology), UL19 (60% homology), UL20 (33% homology), and UL21 (36% homology) polypeptides and the 3'-part of a gene homologous to UL15 (67% homology) of HSV-1. In addition, a consensus sequence for an alphaherpesviral origin of replication was found at the left terminus of the fragment.
Klupp
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BG
Federal Research Centre for Virus Diseases of Animals, Tübingen, Germany.
Kern
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H
Mettenleiter
T C
TC
D001483:Base Sequence; D002874:Chromosome Mapping; D016384:Consensus Sequence; D004261:DNA Replication; D004274:DNA, Recombinant / Q000235:genetics; D004279:DNA, Viral / Q000187:drug effects / Q000235:genetics; D015245:Deoxyribonuclease BamHI / Q000378:metabolism; D005814:Genes, Viral / Q000235:genetics*; D011558:Herpesvirus 1, Suid / Q000235:genetics* / Q000472:pathogenicity*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D012689:Sequence Homology, Nucleic Acid; D018139:Simplexvirus / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics; D014774:Virulence / Q000235:genetics*
D004274:DNA, Recombinant; D004279:DNA, Viral; D014764:Viral Proteins; D015245:Deoxyribonuclease BamHI