[Effect of endothelin on isolated myocytes from rat heart].
The mechanism of endothelin (ET) action on isolated myocytes of rat heart was investigated. Administration of 10(-9)-10(-7) mol/L ET caused dose-dependently severe contracture of myocytes, increase in cytosolic lactate dehydrogenase leakage and increase of cellular total calcium. ET also enhanced 45Ca2+ influx in myocytes, that could be antagonized by calcium channel blocker verapamil. Incubation of Fura-2 -preloaded myocytes with ET would induce a significant increase of cytosolic free Ca2+, and this effect of ET could also be partly antagonized by verapamil. These results suggest that ET enhances Ca2+ influx in myocytes mainly through voltage-dependent calcium channel, by which ET exerts its biological action.
Tang
C S
CS
Laboratory of Cardiopulmonary Endocrinology, Beijing Medical University.
Li
Z P
ZP
Fan
G
G
Tang
J
J
Su
J Y
JY
D000818:Animals; D015220:Calcium Channels / Q000187:drug effects; D002478:Cells, Cultured; D016232:Endothelins / Q000494:pharmacology*; D006321:Heart / Q000187:drug effects*; D008297:Male; D009206:Myocardium / Q000166:cytology*; D051381:Rats; D011919:Rats, Inbred Strains
D015220:Calcium Channels; D016232:Endothelins