title: In vitro activity of silicon carbide whiskers in comparison to other industrial fibers using four cell culture systems.
abstract: Silicon carbide whiskers (SiCW) and continuous glass filaments are important components of composite materials having potentially widespread use in the automotive, aerospace, and power generation industries. We determined the in vitro activity of three well-characterized samples of silicon carbide whiskers and a continuous glass filament sample in four different cellular assays and compared this to the activities of UICC crocidolite, JM Code 100 glass microfiber, and erionite in the same assay systems. The SiCW had a diameter range of 0.32-0.75 microns and a length range of 4.5-20.1 microns. The SiCW was significantly toxic; on a mass basis, one SiCW sample was more toxic than crocidolite; however, JM Code 100 glass microfiber, which is not toxic in vivo (i.e., it does not cause fibrogenesis or carcinogenesis when inhaled), was also more toxic than crocidolite. The glass filament sample was the least cytotoxic of all the samples tested. On a fiber number basis, all three SiCW samples were more toxic than crocidolite. The results of our study showed that SiCW exhibits significant in vitro biological reactivity. Thus, despite the caution that must be exercised in extrpolating the results of in vitro studies to conclusions about in vivo health effects, SiCW should be considered toxic until further toxicological data are available.
authors.affiliation: Inhalation Toxicology Research Institute, Lovelace Biomedical and Environmental Research Institute, Albuquerque, NM 87185.
authors.forename: N F
authors.initials: NF
authors.lastname: Johnson
authors.forename: M D
authors.initials: MD
authors.lastname: Hoover
authors.forename: D G
authors.initials: DG
authors.lastname: Thomassen
authors.forename: Y S
authors.initials: YS
authors.lastname: Cheng
authors.forename: A
authors.initials: A
authors.lastname: Dalley
authors.forename: A L
authors.initials: AL
authors.lastname: Brooks
mesh_terms: D000538:Aluminum Silicates / Q000633:toxicity; D000818:Animals; D001194:Asbestos / Q000633:toxicity; D017638:Asbestos, Crocidolite; D002244:Carbon / Q000737:chemistry / Q000633:toxicity*; D017554:Carbon Compounds, Inorganic; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004847:Epithelial Cells; D004848:Epithelium / Q000187:drug effects; D005898:Glass; D016676:Macrophages, Alveolar / Q000187:drug effects*; D051381:Rats; D012825:Silicon / Q000737:chemistry / Q000633:toxicity*; D017655:Silicon Compounds; D014132:Trachea / Q000187:drug effects*; D017641:Zeolites
chemical_list: D000538:Aluminum Silicates; D017554:Carbon Compounds, Inorganic; D017655:Silicon Compounds; D017638:Asbestos, Crocidolite; D017641:Zeolites; D001194:Asbestos; D002244:Carbon; C022088:silicon carbide; D012825:Silicon