A newly found angiotensin II receptor subtype mediates cyclic GMP formation in differentiated Neuro-2A cells.
In search of the functional role of the newly found angiotensin II (Ang II) binding site which is expressed in differentiated Neuro-2A cells, we found that Ang II causes a marked stimulation of cGMP formation dose-dependently. The stimulation was blocked by the nonselective Ang II receptor antagonist [Sar1,Ile8]Ang II but not by the AT1 antagonist DuP 753 or the AT2 antagonist PD 123319. These results suggest that Ang II increased cGMP level via a new Ang II receptor subtype in differentiated Neuro-2A cells.
Chaki
S
S
Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN 37232.
Inagami
T
T
D000804:Angiotensin II / Q000037:antagonists & inhibitors / Q000378:metabolism / Q000494:pharmacology*; D057911:Angiotensin Receptor Antagonists; D001665:Binding Sites; D001713:Biphenyl Compounds / Q000494:pharmacology; D002454:Cell Differentiation; D006152:Cyclic GMP / Q000378:metabolism*; D007093:Imidazoles / Q000494:pharmacology; D019808:Losartan; D009447:Neuroblastoma / Q000378:metabolism* / Q000473:pathology; D011725:Pyridines / Q000494:pharmacology; D011945:Receptors, Angiotensin / Q000378:metabolism*; D013777:Tetrazoles / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D057911:Angiotensin Receptor Antagonists; D001713:Biphenyl Compounds; D007093:Imidazoles; D011725:Pyridines; D011945:Receptors, Angiotensin; D013777:Tetrazoles; D000804:Angiotensin II; C073402:PD 123319; D006152:Cyclic GMP; D019808:Losartan