Proteins released from vaccinia cores are probably not involved in protein synthesis inhibition in vitro.
1. It is known that vaccinia core proteins are released into the supernatant fraction when cores are incubated under appropriate conditions. When prepared in the absence of viral transcription this fraction inhibits in vitro protein synthesis. 2. We show here that after incubation, the cores loose the capability to inhibit protein synthesis. Furthermore, we show that no inhibition of translation is observed with supernatant fractions prepared from transcribing cores. 3. SDS-PAGE analysis of the supernatant fraction of cores obtained in the presence and absence of viral transcription indicated that their protein composition is similar and that approximately 17 peptides are released from the cores and that 4 are phosphorylated. 4. We conclude that the proteins associated with vaccinia cores and released in the presence of ribonucleotides are not involved in protein synthesis inhibition.
Dâmaso
C R
CR
Laboratório de Biologia Molecular de Vírus, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Brasil.
Moussatché
N
N
D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D006160:Guanosine Triphosphate / Q000494:pharmacology; D006367:HeLa Cells; D006801:Humans; D011500:Protein Synthesis Inhibitors; D011817:Rabbits; D012265:Ribonucleotides / Q000494:pharmacology; D014158:Transcription, Genetic; D014616:Vaccinia virus / Q000187:drug effects / Q000378:metabolism*; D014758:Viral Core Proteins / Q000096:biosynthesis*
D011500:Protein Synthesis Inhibitors; D012265:Ribonucleotides; D014758:Viral Core Proteins; D006160:Guanosine Triphosphate; D000255:Adenosine Triphosphate