title: Phosphotransferase activity associated with rat osseous plate alkaline phosphatase: a possible role in biomineralization.
abstract: 1. Alkaline phosphatase from rat osseous plate catalyzed the transfer of phosphate from p-nitrophenylphosphate to glycerol, ethanolamines, Tris, glucose and 1-amino-1-methyl-2-propanol, in a wide range of pH. Serine did not stimulate phosphotransferase activity of the enzyme. 2. The best phosphotransferase acceptors were diethanolamine and glycerol while glucose was the poorest phosphotransferase acceptor used. 3. Diethanolamine and glycerol affected both VM and KM of p-nitrophenylphosphate hydrolysis with activation constants (KA) of 0.25 and 0.85 M, respectively. 4. A kinetic model was proposed for the phosphotransferase reaction observed with alkaline phosphatase from rat osseous plates.
authors.affiliation: Departamento de Química-Faculdade de Filosofia Ciências e Letras, Universidade de São Paulo, Ribeirão Preto, Brazil.
authors.forename: J M
authors.initials: JM
authors.lastname: Pizauro
authors.forename: P
authors.initials: P
authors.lastname: Ciancaglini
authors.forename: F A
authors.initials: FA
authors.lastname: Leone
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism*; D000818:Animals; D002113:Calcification, Physiologic; D004983:Ethanolamines / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism; D006132:Growth Plate / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D009994:Osmolar Concentration; D010770:Phosphotransferases / Q000378:metabolism*; D051381:Rats
chemical_list: D004983:Ethanolamines; C020283:diethanolamine; D010770:Phosphotransferases; D000469:Alkaline Phosphatase; D005990:Glycerol