Erythrocytes from magnesium-deficient hamsters display an enhanced susceptibility to oxidative stress.
Previous studies in our laboratory have indicated a role for free radical participation in magnesium deficiency cardiomyopathy. We have demonstrated the ability of various antioxidant drugs and nutrients to protect against magnesium deficiency-induced myocardial injury. In this study, we have examined erythrocytes from normal and magnesium-deficient animals and compared their susceptibility to an in vitro oxidative stress. Syrian male hamsters were placed on either magnesium-deficient or magnesium-supplemented diets. Animals from each group also received vitamin E in doses of 10 and 25 mg as subcutaneous implants. Erythrocytes obtained after 14 days on the diet were exposed to an exogenous hydroxyl (.OH) radical generating system (dihydroxyfumarate not equal to Fe3+ ADP) at 37 degrees C for 20 min. Erythrocyte crenation was observed and quantified by scanning electron microscopy. Lipid peroxidation, hemolysis (%), and intracellular glutathione levels were determined. In addition, serum lipid changes and membrane phospholipids were characterized. Our data demonstrate that erythrocytes from magnesium-deficient animals are more susceptible to free radical injury, supporting our hypothesis that magnesium deficiency reduces the threshold antioxidant capacity.
Freedman
A M
AM
Department of Medicine, George Washington University Medical Center, Washington, DC 20037.
Mak
I T
IT
Stafford
R E
RE
Dickens
B F
BF
Cassidy
M M
MM
Muesing
R A
RA
Weglicki
W B
WB
D000244:Adenosine Diphosphate / Q000494:pharmacology; D000818:Animals; D002712:Chlorides; D002784:Cholesterol / Q000097:blood; D006224:Cricetinae; D004910:Erythrocyte Membrane / Q000737:chemistry; D004912:Erythrocytes / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D005290:Ferric Compounds / Q000494:pharmacology; D005650:Fumarates / Q000494:pharmacology; D005978:Glutathione / Q000097:blood; D006461:Hemolysis; D006878:Hydroxides / Q000097:blood; D017665:Hydroxyl Radical; D066298:In Vitro Techniques; D007502:Iron Chelating Agents / Q000494:pharmacology; D015227:Lipid Peroxidation; D008275:Magnesium Deficiency / Q000097:blood*; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D008563:Membrane Lipids / Q000097:blood; D008647:Mesocricetus; D008855:Microscopy, Electron, Scanning; D010743:Phospholipids / Q000097:blood; D012016:Reference Values; D013481:Superoxides / Q000097:blood; D014280:Triglycerides / Q000097:blood
D002712:Chlorides; D005290:Ferric Compounds; D005650:Fumarates; D006878:Hydroxides; D007502:Iron Chelating Agents; D008563:Membrane Lipids; D010743:Phospholipids; D014280:Triglycerides; D013481:Superoxides; C005998:dihydroxyfumarate; D017665:Hydroxyl Radical; D008315:Malondialdehyde; D000244:Adenosine Diphosphate; D002784:Cholesterol; D005978:Glutathione; C024555:ferric chloride