A monoclonal anti-idiotypic 'internal image' antibody that recognizes the A1 adenosine receptor potentiates the alpha 1-adrenergic activation of phospholipase C in primary cultures of mouse striatal astrocytes.
To determine which subtype of adenosine receptor mediates the potentiating effect of 2-chloroadenosine on the noradrenaline-induced inositol-phosphate formation, we used the monoclonal anti-idiotypic antibody AA1 that acts as an 'internal image' of adenosine and specifically recognizes the A1 adenosine receptor. In cultured mouse striatal astrocytes, AA1 increased the noradrenaline-evoked inositol phosphate (IP) accumulation, thus demonstrating a biological activity of an anti-idiotypic antibody. This effect was inhibited by PACPX, a selective A1 antagonist. Inhibitors of phospholipase A2 activity prevented the potentiation. These results establish the involvement of A1 adenosine receptors in the modulation of phospholipase C activity.
el-Etr
M
M
INSERM U.33, Lab Hormones, Kremlin-Bicêtre, France.
Lombes
M
M
Baulieu
E E
EE
Erlanger
B F
BF
D015762:2-Chloroadenosine / Q000494:pharmacology; D015117:5,8,11,14-Eicosatetraynoic Acid / Q000494:pharmacology; D000241:Adenosine / Q000031:analogs & derivatives / Q000494:pharmacology; D019830:Adenosine-5'-(N-ethylcarboxamide); D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology*; D001253:Astrocytes / Q000187:drug effects / Q000201:enzymology*; D002478:Cells, Cultured; D003342:Corpus Striatum / Q000166:cytology / Q000187:drug effects / Q000201:enzymology*; D004789:Enzyme Activation / Q000187:drug effects; D005260:Female; D051379:Mice; D009638:Norepinephrine / Q000494:pharmacology; D011247:Pregnancy; D011796:Quinacrine / Q000494:pharmacology; D011942:Receptors, Adrenergic, alpha / Q000502:physiology*; D011983:Receptors, Purinergic / Q000187:drug effects / Q000276:immunology*; D010738:Type C Phospholipases / Q000378:metabolism*; D014970:Xanthines / Q000494:pharmacology
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D011942:Receptors, Adrenergic, alpha; D011983:Receptors, Purinergic; D014970:Xanthines; D015117:5,8,11,14-Eicosatetraynoic Acid; D015762:2-Chloroadenosine; D019830:Adenosine-5'-(N-ethylcarboxamide); D010738:Type C Phospholipases; C046379:1,3-dipropyl-8-(2-amino-4-chlorophenyl)xanthine; D011796:Quinacrine; D000241:Adenosine; D009638:Norepinephrine