A theoretical method to predict DNA permutation gel electrophoresis from the sequence.
The gel electrophoretic permutation assays of DNA fragments experimentally investigated by different authors were theoretically reproduced using our theoretical model of sequence-dependent curvature. The general pattern of agreement obtained suggests that our method can be usefully adopted as an alternative to the experimental assay, in particular where the lack of a sufficient number of unique restriction sites in the fragment prevents the correct localization of the main bend site.
Boffelli
D
D
Dipartimento di Chimica, Università di Roma, La Sapienza, Italy.
De Santis
P
P
Palleschi
A
A
Risuleo
G
G
Savino
M
M
D004247:DNA / Q000737:chemistry*; D004279:DNA, Viral / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D008956:Models, Chemical; D009690:Nucleic Acid Conformation; D013539:Simian virus 40 / Q000235:genetics
D004279:DNA, Viral; D004247:DNA