title: Analysis of the UL36 open reading frame encoding the large tegument protein (ICP1/2) of herpes simplex virus type 1.
abstract: Using peptide antisera specific for regions within the N terminus and C terminus of the predicted UL36 gene product, immunoblotting experiments were performed to demonstrate definitively that ICP1/2 is encoded by the UL36 gene. These data also suggest that both the cell- and the virion-associated forms of ICP1/2 are colinear with the complete predicted amino acid sequence of the UL36 gene. Computer-assisted analyses of the predicted amino acid sequence of the UL36 gene revealed the presence of two putative leucine zipper-type motifs and a potential ATP-binding domain. The possible functions of these consensus domains will also be discussed.
authors.affiliation: Department of Biochemistry and Molecular Biology, Louisiana State University Medical Center, Shreveport 71130-3932.
authors.forename: D S
authors.initials: DS
authors.lastname: McNabb
authors.forename: R J
authors.initials: RJ
authors.lastname: Courtney
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002460:Cell Line; D004268:DNA-Binding Proteins / Q000235:genetics*; D005814:Genes, Viral; D006801:Humans; D007106:Immune Sera; D016350:Leucine Zippers / Q000235:genetics; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D012091:Repetitive Sequences, Nucleic Acid; D018139:Simplexvirus / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics*; D014771:Virion / Q000235:genetics
chemical_list: D004268:DNA-Binding Proteins; D007106:Immune Sera; C078035:UL36 protein, Human herpesvirus 1; D014764:Viral Proteins