[Mechanism of the photosensitive effects of methylene blue in the inhibition of DNA synthesis in cancer cells].
In the present study, we found that various concentrations of methylene blue (38, 3.8, 0.38, 0.038, 0.0038 mumol/L) significantly inhibited DNA synthesis in ascites hepatoma (AH) cells. The inhibition increased with the concentration of methylene blue as well as with irradiation time. Photosensitization of methylene blue was not due to 1O2, OH., or O2.-, but was closely related to H2O2. The mechanism of inhibition of DNA synthesis may be attributed to the damage of DNA replication template.
Zhou
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X
Department of Biology, Lanzhou University.
Zheng
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R
D000818:Animals; D004249:DNA Damage / Q000528:radiation effects*; D004273:DNA, Neoplasm / Q000096:biosynthesis*; D008027:Light; D008114:Liver Neoplasms, Experimental / Q000473:pathology*; D008751:Methylene Blue / Q000494:pharmacology*; D051379:Mice; D011838:Radiation-Sensitizing Agents / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D004273:DNA, Neoplasm; D011838:Radiation-Sensitizing Agents; D008751:Methylene Blue