Effects of nifedipine and nicardipine on glucagon-stimulated gluconeogenesis in primary cultures of rat hepatocytes.
The effects of nifedipine and nicardipine on glucagon-stimulated gluconeogenesis from lactate were examined in primary cultures of rat hepatocytes. Nifedipine and nicardipine (10(-7)-10(-5) M) significantly potentiated the glucagon-stimulated gluconeogenesis from lactate by increasing intracellular cAMP levels. In contrast, diltiazem and verapamil did not potentiate the glucagon-stimulated gluconeogenesis. 1-Methyl-3-isobutylxanthine and papaverine also potentiated the glucagon-stimulated gluconeogenesis from lactate. On the basis of these results, possible mechanisms by which nifedipine and nicardipine potentiate the glucagon-stimulated gluconeogenesis will be discussed.
Ogihara
M
M
Biochemical Pharmacology Group, Faculty of Pharmaceutical Sciences, Josai University, Saitama, Japan.
D000818:Animals; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000032:analysis; D005934:Glucagon / Q000494:pharmacology; D005943:Gluconeogenesis / Q000187:drug effects*; D005947:Glucose / Q000032:analysis; D008099:Liver / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D009529:Nicardipine / Q000494:pharmacology*; D009543:Nifedipine / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains
D005934:Glucagon; D009529:Nicardipine; D000242:Cyclic AMP; D009543:Nifedipine; D005947:Glucose