Cyclic adenosine 3'5'-monophosphate and the relaxant action of relaxin in the rat uterus in vivo.
The relationship between relaxant actions of relaxin in the uterus and changes in uterine cAMP concentrations was assessed in anaesthetized bilaterally ovariectomized nonpregnant rats. Relaxin i.v. bolus (5 micrograms kg-1) did not change cAMP concentrations but inhibited uterine contractions with rapid onset. Uterine contractions were significantly reduced by 30-70% for 60 min. Relaxin (50 micrograms kg-1) produced a short-lived (up to 5 min) and small (up to 3.2-fold) increase in cAMP concentrations plus a marked (90%) and prolonged inhibition of uterine contractions (70-90% over 60 min). Salbutamol (an agonist at beta 2-adrenoceptors, 100 and 500 micrograms kg-1) produced a similar degree and time course of inhibition of uterine contractions to that of relaxin but a more marked (19-fold) increase in cAMP concentrations. Glibenclamide, a blocker of ATP-sensitive potassium channels, which has been shown to antagonize relaxin as a uterine relaxant, did not prevent the relaxin-induced rise in cAMP concentrations. It is suggested that the uterine relaxant action of relaxin may not result from an increase in uterine cAMP concentrations.
Downing
S J
SJ
Department of Physiological Sciences, Medical School, Manchester University, UK.
McIlwrath
A
A
Hollingsworth
M
M
D000420:Albuterol / Q000494:pharmacology; D000704:Analysis of Variance; D000818:Animals; D000242:Cyclic AMP / Q000096:biosynthesis*; D004305:Dose-Response Relationship, Drug; D005260:Female; D005905:Glyburide / Q000494:pharmacology; D010052:Ovariectomy; D015221:Potassium Channels / Q000187:drug effects / Q000502:physiology; D051381:Rats; D018343:Receptors, Adrenergic, beta-2 / Q000502:physiology; D012065:Relaxin / Q000502:physiology*; D013997:Time Factors; D014590:Uterine Contraction / Q000187:drug effects / Q000502:physiology*; D014599:Uterus / Q000378:metabolism
D015221:Potassium Channels; D018343:Receptors, Adrenergic, beta-2; D012065:Relaxin; D000242:Cyclic AMP; D000420:Albuterol; D005905:Glyburide