Detection of infectious bursal disease viruses using in situ hybridization and non-radioactive probes.
The in situ hybridization assay was developed for the detection of infectious bursal disease virus (IBDV) infections in chickens. Bursal tissue samples were harvested 4 days following infection with the ST-C, MD, E, IN, or SAL IBDV strain. The cDNA clones STC-243, located on genome segment A, and STC-119, located on genome segment B, were used to prepare non-radioactive probes. Probes were labeled with digoxigenin and detected the homologous ST-C virus and also heterologous viruses in bursal tissue sections. No positive cells were observed in tissue sections from uninfected control chickens.
Jackwood
D J
DJ
Department of Veterinary Preventive Medicine, Ohio Agricultural Research and Development Center, Ohio State University, Wooster 44691.
Swayne
D E
DE
Fisk
R J
RJ
D000818:Animals; D002060:Bursa of Fabricius / Q000382:microbiology / Q000473:pathology; D002645:Chickens; D015342:DNA Probes; D004279:DNA, Viral; D007243:Infectious bursal disease virus / Q000235:genetics / Q000302:isolation & purification*; D009693:Nucleic Acid Hybridization; D011201:Poultry Diseases / Q000382:microbiology*; D012088:Reoviridae Infections / Q000382:microbiology / Q000662:veterinary*
D015342:DNA Probes; D004279:DNA, Viral