[Effect of NO-like relaxing factor (NO-LRF) in rat tourniquet shock].
On tourniquet shock (ToS) rat model, it was found that the reactivity of isolated perfused aortic ring to noradrenaline was decreased, while the cGMP content of the aortic tissue was increased. These ToS-induced changes could be potentiated or attenuated respectively by perfusion with NO-precursor, L-arginine, or NO-synthesis inhibitor L-NNA independent of the presence of vascular endothelium. Guanylate cyclase inhibitor, methylene blue, could also attenuate the aortic reactivity. All these results suggest that the aortic musculature can produce a NO-LRF factor capable of lowering the vascular reactivity of the ToS animals. That L-arginine can ameliorate while L-NNA can exacerbate ToS, suggest that NO-LRF do play an adaptive role in the protective mechanism of the organism during ToS.
Zhang
L Y
LY
Laboratory of Cardiopulmonary Endocrinology, Beijing Medical University.
Dong
S Y
SY
Yang
J
J
Tang
J
J
Su
J Y
JY
Tang
C S
CS
D000818:Animals; D001011:Aorta / Q000378:metabolism; D001120:Arginine / Q000031:analogs & derivatives* / Q000494:pharmacology; D001794:Blood Pressure / Q000187:drug effects; D006152:Cyclic GMP / Q000378:metabolism; D066298:In Vitro Techniques; D008297:Male; D009569:Nitric Oxide / Q000378:metabolism* / Q000502:physiology; D019335:Nitroarginine; D051381:Rats; D017208:Rats, Wistar; D012769:Shock / Q000209:etiology / Q000378:metabolism*; D014111:Tourniquets; D014665:Vasodilator Agents
D014665:Vasodilator Agents; D019335:Nitroarginine; D009569:Nitric Oxide; D001120:Arginine; D006152:Cyclic GMP