[Formation of superoxide radicals in membranes of subcellular organelles in regenerating liver].
A correlation between the changes in the rates of superoxide radical generation, upsilon, in microsomes, mitochondria, and nuclei and the Cu, Zn- and Mn-SOD activities in rat liver during the first 5 days after partial hepatectomy, has been studied. Level of upsilon in microsomal and mitochondrial membranes in the regeneration process was reduced. The Cu, Zn- and Mn-SOD activities changed in an extreme and antibate manner: the former was characterized by a minimum, whereas the latter-by a maximum with an extreme on the 3rd day after surgery. Analysis of the correlation between the values of upsilon in the nuclear membranes and cell cycle stages (on a literary basis) revealed that the upsilon was decreased 2 times on the stage of DNA synthesis. When mitosis was at maximum, upsilon showed a 4-5-fold increase in comparison with the control, the Cu, Zn-SOD activity being essentially unchanged. A role of SOD and O2-. in cell division is postulated. O2-. is assumed to play a role in gene expression, disassembly, and regeneration of the nuclear membrane; that of SOD is thought to consist in regulation of the proliferative activity.
Vartanian
L S
LS
Sadovnikova
I P
IP
Gurevich
S M
SM
Sokolova
I S
IS
D000818:Animals; D004247:DNA / Q000096:biosynthesis; D005260:Female; D005609:Free Radicals; D007425:Intracellular Membranes / Q000378:metabolism*; D007700:Kinetics; D008099:Liver / Q000201:enzymology / Q000502:physiology; D008115:Liver Regeneration; D015388:Organelles / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013482:Superoxide Dismutase / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism*
D005609:Free Radicals; D013481:Superoxides; D004247:DNA; D013482:Superoxide Dismutase
Obrazovanie superoksidnykh radikalov v membranakh subkletochnykh organell regeneriruiushcheĭ pecheni.