title: Coxsackie B1 virus-induced changes in cell membrane-associated functions are not responsible for altered sensitivity to bacterial invasiveness.
abstract: To analyze the possible mechanisms by which coxsackie B1 virus infection affects the invasiveness of Shigella flexneri, we have studied the influence of intracellular levels of Na+ and K+, ATPase activity, cytoplasmic membrane potential, cAMP level and cell communication through gap junctions. 3h after adsorption of viable or UV-inactivated coxsackie B1 virus the Na(+)-K+ gradient of the cell collapsed, ATPase activity decreased, the cytoplasmic membranic potential-dependent tetraphosphonium ion uptake were reduced. No changes in cAMP or intercellular cell communication were observed. S. flexneri invasiveness in HEp-2 cell pretreated with viable or UV-inactivated coxsackie B 1 virus was enhanced, but bacterial invasiveness was unchanged in K(+)-depleted HEp-2 cells, cell cultures with high intracellular Na+ content or ouabain pre-treated cells compared to control cells. We found no correlation between the enhanced bacterial invasiveness in the early phase of coxsackie B 1 virus infection in HEp-2 cell cultures and intracellular K+ depletion, high intracellular Na+ content, inhibited Na(+)-K+ ATPase activity or membranic depolarization.
authors.affiliation: Kaptein W. Wilhelmsen og Frues Bakteriologiske Institutt, University of Oslo, Norway.
authors.forename: K
authors.initials: K
authors.lastname: Modalsli
authors.forename: G
authors.initials: G
authors.lastname: Bukholm
authors.forename: S O
authors.initials: SO
authors.lastname: Mikalsen
authors.forename: M
authors.initials: M
authors.lastname: Degré
mesh_terms: D002450:Cell Communication; D002460:Cell Line; D002462:Cell Membrane / Q000382:microbiology* / Q000502:physiology; D000242:Cyclic AMP / Q000378:metabolism; D029822:Enterovirus B, Human / Q000502:physiology* / Q000528:radiation effects; D007365:Intercellular Junctions / Q000378:metabolism; D008564:Membrane Potentials; D009861:Onium Compounds / Q000378:metabolism; D009943:Organophosphorus Compounds / Q000378:metabolism; D010042:Ouabain / Q000494:pharmacology; D011188:Potassium / Q000378:metabolism; D012763:Shigella flexneri / Q000502:physiology*; D015398:Signal Transduction; D012964:Sodium / Q000378:metabolism; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D014466:Ultraviolet Rays
chemical_list: D009861:Onium Compounds; D009943:Organophosphorus Compounds; D010042:Ouabain; D012964:Sodium; D000242:Cyclic AMP; D000254:Sodium-Potassium-Exchanging ATPase; D011188:Potassium; C013289:tetraphenylphosphonium