The monocytic cell line SKM-1 strongly expresses the myeloperoxidase gene.
A newly established human monocytic cell line, SKM-1, showed strong expression of myeloperoxidase mRNA, to the same extent as in HL-60 cells. We studied the cell morphology and myeloperoxidase expression of this cell line, which was established from a patient with myelodysplastic syndrome who had an abnormal chromosome on the upstream region of 17p13. Electron micrographs showed the cells to have a fragile and irregular cell surface. SKM-1 cells were peroxidase-positive. About 60% of myeloperoxidase (MPO) was released to the culture fluid from SKM-1 cells but only a few percent of MPO was released from HL-60 cells into the culture fluid. The predominant mRNA size of SKM-1 myeloperoxidase was 3.3 kb although there was a smaller size as well. Fluorescent in situ hybridization of MPO mRNA showed strong staining in 5% to 10% of SKM-1 cells and of bone marrow cells from patients with myelogenous leukemia, while all cells from HL-60 were positive.
Kawaguchi
R
R
Genetic Research Laboratory, SRL, Tokyo, Japan.
Hosokawa
Y
Y
Komine
A
A
Tsutsumi
M
M
Hikiji
K
K
Ishida-Okawara
A
A
Suzuki
K
K
Nakagawa
T
T
Yamaguchi
K
K
D000208:Acute Disease; D000368:Aged; D000754:Anemia, Refractory, with Excess of Blasts / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D015152:Blotting, Northern; D002886:Chromosomes, Human, Pair 17; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D004915:Leukemia, Erythroblastic, Acute / Q000235:genetics / Q000378:metabolism; D007951:Leukemia, Myeloid / Q000235:genetics / Q000378:metabolism; D008297:Male; D009195:Peroxidase / Q000032:analysis*; D012334:RNA, Neoplasm / Q000032:analysis*; D014407:Tumor Cells, Cultured
D012334:RNA, Neoplasm; D009195:Peroxidase