[Titration of SH-groups of histone H3 in a DNP preparation of normal and neoplastic animal cells with a mercury-containing spin marker and with a DTNB preparation].
DNP samples isolated from the cells of calf thymus and Ehrlich ascite carcinoma of mice were examined. SH-groups of histone H3 of chromatin from these cells were titrated with mercury-containing spin label and with DTNB under joint action of different salt and sarcosyl concentrations on DNP. The results revealed differences in accessibility and titration of histone H3 SH-groups in DNP of normal and tumor cells with DTNB, as well as in molecular dynamics of the mercury-containing spin label introduced to these SH-groups.
Mil'
E M
EM
Voronina
S S
SS
Biniukov
V I
VI
D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D003852:Deoxyribonucleoproteins / Q000737:chemistry*; D004228:Dithionitrobenzoic Acid / Q000737:chemistry; D004578:Electron Spin Resonance Spectroscopy; D006657:Histones / Q000032:analysis*; D008628:Mercury / Q000737:chemistry*; D051379:Mice; D013113:Spin Labels; D013438:Sulfhydryl Compounds / Q000737:chemistry*; D014407:Tumor Cells, Cultured
D003852:Deoxyribonucleoproteins; D006657:Histones; D013113:Spin Labels; D013438:Sulfhydryl Compounds; D004228:Dithionitrobenzoic Acid; D008628:Mercury
Titrovanie SH-grupp gistona N3 v DNP normal'nykh i opukholevykh kletok zhivotnykh rtut'soderzhashcheĭ spinovoĭ metkoĭ i preparatom DTNB.