title: [The pharmacological characteristics, immunochemical identification and study of the location of quisqualate receptors in the rat and human brains].
abstract: The kinetics of [3H]-L-glutamate binding to brain synaptic membranes (SM) and to glutamate-binding proteins (GBP) was determined with agonist and monoclonal antibodies (MAbs). It was revealed, that rat and human brain GBP have individual protein components with M(r) from 14 to 92 kDa. Quisqualate inhibited [3H]-L-glutamate binding to solubilized and to purified 68 kDa protein component. MAbs have the most activity, and NMDA was failure. It has been shown that 68 kDa component antigen determinants are similar to those of bovine, frog and rat brain synaptic membranes. Anti-GBP monoclonal antibodies blocked functional non-NMDA receptors in isolated frog spinal cord. Immunocytochemistry was done on rat and human brain sections. Distribution of quisqualate receptors was determined with light and electron microscopy. Some properties of vertebrate CNS non-NMDA receptors are discussed.
authors.forename: S A
authors.initials: SA
authors.lastname: Dambinova
authors.forename: Iu V
authors.initials: IuV
authors.lastname: Bobryshev
authors.forename: A I
authors.initials: AI
authors.lastname: Gorodinskiĭ
authors.forename: M N
authors.initials: MN
authors.lastname: Margulis
authors.forename: A O
authors.initials: AO
authors.lastname: Nikitin
authors.forename: E A
authors.initials: EA
authors.lastname: Orlova
authors.forename: I Iu
authors.initials: IIu
authors.lastname: Pavlov
authors.forename: A Iu
authors.initials: AIu
authors.lastname: Savinov
mesh_terms: D000328:Adult; D000818:Animals; D000911:Antibodies, Monoclonal; D001921:Brain / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D005971:Glutamates / Q000276:immunology / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D008024:Ligands; D008565:Membrane Proteins / Q000276:immunology / Q000378:metabolism; D016253:Microscopy, Immunoelectron; D011485:Protein Binding / Q000187:drug effects; D016318:Quisqualic Acid / Q000378:metabolism*; D011895:Rana ridibunda; D051381:Rats; D018091:Receptors, AMPA; D017470:Receptors, Glutamate / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure
chemical_list: D000911:Antibodies, Monoclonal; D005971:Glutamates; D008024:Ligands; D008565:Membrane Proteins; D018091:Receptors, AMPA; D017470:Receptors, Glutamate; D016318:Quisqualic Acid
vernacular_title: Farmakologicheskaia kharakteristika, immunokhimicheskaia identifikatsiia i issledovanie lokalizatsii kviskvalatnykh retseptorov golovnogo mozga krysy i cheloveka.