title: Plasmodium falciparum and Plasmodium chabaudi: characterization of glycosylphosphatidylinositol-degrading activities.
abstract: Merozoites of malaria parasites have a membrane-bound serine protease whose solubilization and subsequent activity depend on a parasite-derived glycosylphosphatidylinositol-phospholipase C (GPI-PLC). The GPI-degrading activities from both Plasmodium falciparum and Plasmodium chabaudi have been characterized and partially purified by phenylboronate chromatography. They are membrane-bound, developmentally regulated, calcium-independent enzymes and as such they resemble GPI-PLC of Trypanosoma brucei. Furthermore, a T. brucei GPI-PLC-specific monoclonal antibody (mAT3) immunoprecipitates the plasmodial GPI-degrading activity. Thin-layer chromatography is suggestive of two activities: a GPI-PLC and a phospholipase A.
authors.affiliation: Department of Immunology, Institut Pasteur, Paris, France.
authors.forename: C
authors.initials: C
authors.lastname: Braun-Breton
authors.forename: T
authors.initials: T
authors.lastname: Blisnick
authors.forename: P
authors.initials: P
authors.lastname: Barbot
authors.forename: R
authors.initials: R
authors.lastname: Bülow
authors.forename: L
authors.initials: L
authors.lastname: Pereira da Silva
authors.forename: G
authors.initials: G
authors.lastname: Langsley
mesh_terms: D000818:Animals; D003429:Cross Reactions; D006017:Glycolipids / Q000378:metabolism*; D054800:Glycosylphosphatidylinositol Diacylglycerol-Lyase; D017261:Glycosylphosphatidylinositols; D043265:Phosphatidylinositol Diacylglycerol-Lyase; D010716:Phosphatidylinositols / Q000378:metabolism*; D010741:Phospholipases A / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D010727:Phosphoric Diester Hydrolases / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D016788:Plasmodium chabaudi / Q000201:enzymology*; D010963:Plasmodium falciparum / Q000201:enzymology*; D014346:Trypanosoma brucei brucei / Q000201:enzymology; D010738:Type C Phospholipases / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D006017:Glycolipids; D017261:Glycosylphosphatidylinositols; D010716:Phosphatidylinositols; D010741:Phospholipases A; D010727:Phosphoric Diester Hydrolases; D010738:Type C Phospholipases; D043265:Phosphatidylinositol Diacylglycerol-Lyase; D054800:Glycosylphosphatidylinositol Diacylglycerol-Lyase