title: Nonselective utilization of the endomannosidase pathway for processing glycoproteins by human hepatoma (HepG2) cells.
abstract: Endo-alpha-D-mannosidase, a Golgi-situated processing enzyme, provides a glucosidase-independent pathway for the formation of complex N-linked oligosaccharides of glycoproteins (Moore, S. E. H., and Spiro, R. G. (1990) J. Biol. Chem. 265, 13104-13112). The present report demonstrates that at least five distinct glycoproteins secreted by HepG2 cells (alpha 1-antitrypsin, transferrin, alpha 1-acid glycoprotein, alpha 1-antichymotrypsin, and alpha-fetoprotein) as well as cell surface components can effectively utilize this alternate processing route. During a castanospermine (CST)-imposed glucosidase blockade, these glycoproteins apparently were produced with their usual complement of complex carbohydrate units, and upon addition of the mannosidase I inhibitor, 1-deoxymannojirimycin (DMJ), to prevent further processing of deglucosylated N-linked oligosaccharides, Man6-8GlcNAc, but not Man9GlcNAc, were identified; the Man8GlcNAc component occurred as the characteristic isomer generated by endomannosidase cleavage. Although the endomannosidase-mediated deglucosylation pathway appeared to be nonselective, a differential inhibitory effect on the secretion of the various glycoproteins was noted in the presence of CST which was directly related to the number of their N-linked oligosaccharides, ranging from minimal in alpha-fetoprotein to substantial (approximately 65%) in alpha 1-acid glycoprotein. Addition of DMJ to CST-incubated cells did not further decrease secretion of the glycoproteins, although processing was now arrested at the polymannose stage, and a portion of the oligosaccharides were still in the glucosylated form. These latter findings indicate that complex carbohydrate units are not required for secretion of these glycoproteins and that any effect which glucose residues exert on their intracellular transit would be related to movement from the endoplasmic reticulum to the Golgi compartment.
authors.affiliation: Department of Biological Chemistry and Medicine, Harvard Medical School, Boston, Massachusetts.
authors.forename: C
authors.initials: C
authors.lastname: Rabouille
authors.forename: R G
authors.initials: RG
authors.lastname: Spiro
mesh_terms: D017485:1-Deoxynojirimycin; D006528:Carcinoma, Hepatocellular / Q000378:metabolism*; D002855:Chromatography, Thin Layer; D004591:Electrophoresis, Polyacrylamide Gel; D005944:Glucosamine / Q000031:analogs & derivatives / Q000494:pharmacology; D006023:Glycoproteins / Q000378:metabolism*; D006026:Glycoside Hydrolases / Q000037:antagonists & inhibitors; D006596:Hexosaminidases / Q000378:metabolism; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism*; D008361:Mannosidases / Q000378:metabolism*; D011233:Precipitin Tests; D014407:Tumor Cells, Cultured
chemical_list: D006023:Glycoproteins; D017485:1-Deoxynojirimycin; D006026:Glycoside Hydrolases; D006596:Hexosaminidases; D008361:Mannosidases; D005944:Glucosamine