Cytomegalovirus genomes demonstrated by polymerase chain reaction in synovial fluid from rheumatoid arthritis patients.
Synovial fluid from 20 patients with rheumatoid arthritis was analyzed to detect human cytomegalovirus (CMV) genomic material using polymerase chain reaction. Of 20 samples tested, 9 were positive for CMV by either ethidium bromide staining or Southern blotting. In contrast, CMV was detected in only 2 of 18 control patients with osteoarthritis. These results suggest an etiologic relationship between CMV and rheumatoid arthritis.
Murayama
T
T
Department of Microbiology, Kanazawa Medical University, Ishikawa, Japan.
Jisaki
F
F
Ayata
M
M
Sakamuro
D
D
Hironaka
T
T
Hirai
K
K
Tsuchiya
N
N
Ito
K
K
Furukawa
T
T
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001172:Arthritis, Rheumatoid / Q000150:complications / Q000235:genetics* / Q000382:microbiology; D001483:Base Sequence; D015139:Blotting, Southern; D003587:Cytomegalovirus / Q000235:genetics* / Q000302:isolation & purification; D003586:Cytomegalovirus Infections / Q000150:complications; D004279:DNA, Viral / Q000032:analysis* / Q000235:genetics*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D010003:Osteoarthritis / Q000150:complications / Q000235:genetics / Q000382:microbiology; D016133:Polymerase Chain Reaction; D013582:Synovial Fluid / Q000737:chemistry* / Q000382:microbiology
D004279:DNA, Viral