Effects of chemical reagents on the allergenicity of house dust.
Con A-Sepharose bound and unbound house dust allergens were treated with various protein modifying reagents in order to ascertain the amino acid residues responsible for their allergenicity. Modification of carboxy group and arginine residue of bound fraction led to 90 and 85% loss of allergenicity whereas carboxy group, lysine and tryptophan modified unbound allergen reduced greater than or equal to 85% of activity. Allergenicity of both fractions was highly affected by pronase. On periodate treatment the bound fraction lost its allergenicity more compared to unbound one. Chemical regents had no remarkable effect on the activity of unbound fraction.
Mitra
I
I
Department of Biological Chemistry, Indian Association for the Cultivation of Science, Jadavpur, Calcutta.
Sikdar
S
S
Chatterjee
B P
BP
D000485:Allergens / Q000737:chemistry; D000596:Amino Acids / Q000737:chemistry*; D001120:Arginine / Q000737:chemistry; D004391:Dust / Q000009:adverse effects*; D004797:Enzyme-Linked Immunosorbent Assay; D008239:Lysine / Q000737:chemistry; D010084:Oxidation-Reduction; D010504:Periodic Acid; D014364:Tryptophan / Q000737:chemistry
D000485:Allergens; D000596:Amino Acids; D004391:Dust; D010504:Periodic Acid; D014364:Tryptophan; D001120:Arginine; D008239:Lysine