A recombinant-based feline immunodeficiency virus antibody enzyme-linked immunosorbent assay.
We have developed an antibody detection enzyme-linked immunosorbent assay (ELISA) for the identification of animals infected by feline immunodeficiency virus (FIV). The ELISA solid-phase antigen consists of recombinant FIV gag proteins expressed in bacteria. The proteins are purified from bacterial lysates as insoluble inclusion bodies. In the case of bacterially expressed p24gag, it is shown that all of the linear, sequential epitopes presented by viral p24 during infection are retained. Purified preparations can be substituted for solid-phase whole virus in the IDEXX PetChektm immunoassay. The antibody ELISA duplicates the sensitivity and specificity of the whole virus based PetChek plate assay.
Mermer
B
B
IDEXX Laboratories, Westbrook, ME 04092.
Hillman
P
P
Harris
R
R
Krogmann
T
T
Tonelli
Q
Q
Palin
W
W
Andersen
P
P
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D002415:Cats; D002460:Cell Line; D003001:Cloning, Molecular; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D004926:Escherichia coli / Q000235:genetics; D016181:Feline Acquired Immunodeficiency Syndrome / Q000175:diagnosis*; D015967:Gene Expression Regulation, Viral; D015683:Gene Products, gag / Q000235:genetics / Q000276:immunology*; D015750:Genes, gag; D016089:Immunodeficiency Virus, Feline / Q000235:genetics / Q000276:immunology*; D011993:Recombinant Fusion Proteins / Q000276:immunology / Q000302:isolation & purification; D012680:Sensitivity and Specificity; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology*
D000914:Antibodies, Viral; D015683:Gene Products, gag; D011993:Recombinant Fusion Proteins; D014759:Viral Envelope Proteins; C079587:p15(gag) protein, Feline immunodeficiency virus; C074662:p24 protein, Feline immunodeficiency virus