Inhibition of human immunodeficiency virus (HIV) type 1 multiplication by an avian cellular factor.
A factor secreted from avian cells infected non productively with a non cytopathogenic mutant of vesicular stomatitis virus (VSV ts 1026) interferes with HIV replication in CEM cells and peripheral blood monocytes (PBL). Production of infectious particles is decreased and many virions lack cores and/or spikes. In CEM cells the prmRNA is spliced into 7.5, 4, and 2 kb mRNA. Residual virus contains less env encoded proteins and p 18; p 25 appears as several bands. The processing of tat, rev. and nef proteins differs in treated cells and in controls.
Semmel
M
M
INSERM Unité 268, Hôpital Paul-Brousse, Villejuif, France.
Alileche
A
A
Coulaud
D
D
Aguilar
J
J
Krief
P
P
Jasmin
C
C
D000818:Animals; D015152:Blotting, Northern; D015153:Blotting, Western; D015704:CD4 Antigens / Q000378:metabolism; D016827:CD8 Antigens / Q000378:metabolism; D002460:Cell Line; D002642:Chick Embryo; D016207:Cytokines / Q000378:metabolism / Q000494:pharmacology*; D005347:Fibroblasts / Q000378:metabolism / Q000382:microbiology; D015497:HIV-1 / Q000187:drug effects* / Q000502:physiology / Q000648:ultrastructure; D008213:Lymphocyte Activation; D008854:Microscopy, Electron; D012333:RNA, Messenger / Q000096:biosynthesis / Q000187:drug effects; D012367:RNA, Viral / Q000096:biosynthesis / Q000187:drug effects; D013601:T-Lymphocytes / Q000276:immunology / Q000382:microbiology; D014158:Transcription, Genetic; D014721:Vesicular stomatitis Indiana virus / Q000502:physiology*; D014779:Virus Replication / Q000187:drug effects
D015704:CD4 Antigens; D016827:CD8 Antigens; D016207:Cytokines; D012333:RNA, Messenger; D012367:RNA, Viral