In vitro accurate transcription from the cap site of bovine leukemia virus (BLV) dependent on the BLV-infected cell nuclear lysate.
The cell-free transcriptional system initiating from the cap site in bovine leukemia virus (BLV) LTR by RNA polymerase II was constructed. The transcription was completely dependent on the template DNA and the nuclear lysate isolated from BLV-infected bat lung cells (TB1Lu). The relative transcriptional rates estimated using several deletion mutants around the promoter sequence in BLV LTR as templates closely corresponded to that obtained by transient expression assay in cultured cells using these plasmids and tax-producing plasmid. The partial purification of the factor(s) involving to the transcriptional activation from the nuclear lysate suggested that the factor(s) was different from tax and rex, the regulatory factors encoded on viral genome. The transcription from the caps site of adenovirus E3 was also stimulated in the presence of the nuclear lysate from BLV-infected cells.
Shoji-Tanaka
A
A
Laboratory of Molecular Oncology, Tsukuba Life Science Center, Japan.
Katoh
I
I
Yoshinaka
Y
Y
Ikawa
Y
Y
D000818:Animals; D002457:Cell Extracts; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D001909:Leukemia Virus, Bovine / Q000235:genetics*; D009154:Mutation / Q000235:genetics; D012315:RNA Caps / Q000235:genetics*; D012319:RNA Polymerase II / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*; D015183:Restriction Mapping; D014158:Transcription, Genetic / Q000235:genetics*
D002457:Cell Extracts; D012315:RNA Caps; D011993:Recombinant Fusion Proteins; D012319:RNA Polymerase II