Comparison of a calcitonin gene-related peptide receptor in a human neuroblastoma cell line (SK-N-MC) and a calcitonin receptor in a human breast carcinoma cell line (T47D).
A specific CGRP-binding protein of M(r) 60,000 has been identified in the human neuroblastoma cell line SK-N-MC. After N-deglycosylation a M(r) of 48,000 was found. The M(r) were indistinguishable from those determined in the human cerebellum. Receptor binding of CGRP is coupled to cyclic AMP formation. The latter is antagonized by hCGRP-I8-37. CT and DAPamide interact only minimally with the CGRP receptor, whereas CGRP and DAPamide are full agonists in T47D cells. The CT receptor on human breast cancer cell line T47D is clearly different from the human CGRP receptor.
Muff
R
R
Department of Orthopedic Surgery, University of Zürich, Switzerland.
Stangl
D
D
Born
W
W
Fischer
J A
JA
D000595:Amino Acid Sequence; D001943:Breast Neoplasms; D015740:Calcitonin Gene-Related Peptide / Q000378:metabolism*; D002460:Cell Line; D000242:Cyclic AMP / Q000378:metabolism; D005260:Female; D006801:Humans; D007700:Kinetics; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009447:Neuroblastoma; D018003:Receptors, Calcitonin; D011956:Receptors, Cell Surface / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D012689:Sequence Homology, Nucleic Acid
D018003:Receptors, Calcitonin; D011956:Receptors, Cell Surface; D000242:Cyclic AMP; D015740:Calcitonin Gene-Related Peptide