Infection of the HTLV-I-harbouring T-lymphoblastoid line MT-2 by Epstein-Barr virus.
Epstein-Barr virus (EBV), a ubiquitous human B-lymphotropic virus, is associated with certain lymphoproliferative diseases of T-cell lineage. To understand the mechanism by which EBV infects T cells, we have tested the susceptibility of various human T-cell lines to the virus. We report here that the HTLV-I-harbouring T-lymphoblastoid line MT-2 carries a high level of CD21/EBV receptors on their surface, adsorbs fluorescein isothiocyanate-labeled EBV, and synthesizes virus latent antigens (EBNA-1 and LMP) following EBV infection. Pretreatment of MT-2 cells with anti-CD21 monoclonal antibody OKB7 inhibited the virus binding as well as the synthesis of virus latent antigen. These data suggest that human T-cells can be infected with EBV via functionally active virus receptors.
Koizumi
S
S
Department of Virology, Hokkaido University School of Medicine, Sapporo, Japan.
Zhang
X K
XK
Imai
S
S
Sugiura
M
M
Usui
N
N
Osato
T
T
D000944:Antigens, Differentiation, B-Lymphocyte / Q000378:metabolism; D000956:Antigens, Viral / Q000378:metabolism; D002460:Cell Line; D004854:Herpesvirus 4, Human / Q000254:growth & development* / Q000276:immunology; D015368:Human T-lymphotropic virus 1 / Q000254:growth & development; D006801:Humans; D007158:Immunologic Techniques; D066298:In Vitro Techniques; D011951:Receptors, Complement / Q000378:metabolism; D017464:Receptors, Complement 3d; D013601:T-Lymphocytes / Q000382:microbiology*; D014764:Viral Proteins / Q000378:metabolism
D000944:Antigens, Differentiation, B-Lymphocyte; D000956:Antigens, Viral; D011951:Receptors, Complement; D017464:Receptors, Complement 3d; D014764:Viral Proteins