[The detection of human papillomaviruses in histological preparations by using dot-blot hybridization].
The 56 tissue samples were obtained from female patients with anogenital tumors, benign or malign, subsequently confirmed through pathomorphological analysis. The investigations were aimed at the discovering of HPV presence using hybridization techniques with biotinylated molecular probes and monitorisation through enzymatic reaction. The presence of ADN types 11, 16 and 18 was detected on about 52% of all the cases; the ADN HPV incidence was 57% among the patients with a neoplasm and/or cervix uteri papillomatosis diagnosis.
Anton
G
G
Institute de Virologie Stefan S. Nicolau, Bucarest, Roumanie.
Stoian
M
M
Iliescu
R
R
Popa
L M
LM
Repanovici
R
R
D000328:Adult; D000368:Aged; D001005:Anus Neoplasms / Q000382:microbiology; D001710:Biotin; D015344:DNA Probes, HPV; D004273:DNA, Neoplasm / Q000302:isolation & purification; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D005833:Genital Neoplasms, Female / Q000382:microbiology; D006801:Humans; D015151:Immunoblotting / Q000379:methods*; D008875:Middle Aged; D009693:Nucleic Acid Hybridization / Q000379:methods*; D027383:Papillomaviridae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D010957:Plasmids; D012703:Serotyping; D014412:Tumor Virus Infections / Q000382:microbiology
D015344:DNA Probes, HPV; D004273:DNA, Neoplasm; D004279:DNA, Viral; D001710:Biotin
Détection des papillomavirus humains sur des préparations histologiques à l'aide de l'hybridation dot-blot.