Replication of plasmid-derived human papillomavirus type 11 DNA in cultured keratinocytes.
Plasmid-derived human papillomavirus type 11 (HPV11) DNA has been shown to replicate episomally and semiconservatively following transfection of primary human foreskin keratinocytes. HPV11 DNA was excised from the bacterial plasmid, religated to form circular molecules, and cotransfected along with pSV2Neo. Transfectants were selected and shown to contain replicated episomal HPV DNA. Once selected, HPV11 DNA persists in cells through at least two additional passages.
Mungal
S
S
Department of Oral Biology and Pathology, School of Dental Medicine, State University of New York, Stony Brook 11794-8702.
Steinberg
B M
BM
Taichman
L B
LB
D002478:Cells, Cultured; D004261:DNA Replication; D004279:DNA, Viral / Q000378:metabolism*; D006801:Humans; D015603:Keratinocytes / Q000382:microbiology*; D027383:Papillomaviridae / Q000254:growth & development*; D010957:Plasmids; D012692:Serial Passage; D014162:Transfection; D014412:Tumor Virus Infections
D004279:DNA, Viral