title: Fungal protein enrichment of residual liquor from a sugar-cane waste Saccharum officinarum.
abstract: The use of residual liquor obtained from cane-thrash, a sugar-cane waste Saccharum officinarum, L. , as a protein source was studied. The cane-thrash was available in local sugar-mills and the fungus utilized, the Aspergillus niger IZ-9, was provided by the University of São Paulo and derived from a strain under the number NRRL-337. The liquor was previously hydrolyzed and physicochemical analyses were performed. Total nitrogen and amino acid contents were measured in the final product. The synthetic medium of Pontecorvo et al. modified by Christias et al., was used for growth, maintenance and storage of the strain. The analysis of variance was used to determine the statistical significance of all results. Mean values were compared by the Tukey's test. According to our data, the residual liquor from the prehydrolyzed cane-thrash can be used as a medium for biomass production from the A. niger IZ-9 and that the highest biomass production was obtained when the synthetic medium was substituted for 100% treated liquor added with balanced salts and carbon, and pH adjusted to 5.0. The highest amino acid values were detected in the A. Niger grown in the medium containing 40% treated liquor added with balanced salts and carbon, with pH adjustment to 5.0 with exception of methionine, this fungal protein seems to provide all essential amino acids in a diet, and could certainly be considered of good biological value.
authors.affiliation: Department of Nutrition, Federal University of Pernambuco, Recife, Brazil.
authors.forename: T L
authors.initials: TL
authors.lastname: Stamford
authors.forename: R
authors.initials: R
authors.lastname: de Camargo
mesh_terms: D000596:Amino Acids; D001234:Aspergillus niger / Q000254:growth & development / Q000378:metabolism; D050260:Carbohydrate Metabolism; D003470:Culture Media; D005516:Food Microbiology; D005656:Fungal Proteins / Q000096:biosynthesis / Q000302:isolation & purification; D006863:Hydrogen-Ion Concentration; D007220:Industrial Waste; D010936:Plant Extracts / Q000737:chemistry*; D010940:Plant Proteins / Q000302:isolation & purification*; D010945:Plants, Edible
chemical_list: D000596:Amino Acids; D003470:Culture Media; D005656:Fungal Proteins; D007220:Industrial Waste; D010936:Plant Extracts; D010940:Plant Proteins