Sputum cysteinyl-leukotriene levels correlate with the severity of pulmonary disease in children with cystic fibrosis.
Sputum samples from 13 children with cystic fibrosis (CF) were analyzed for leukotrienes (LTs) LTB4, LTC4, LTD4, and LTE4. Distribution of LTB4 appeared to be normal, and of cysteinyl-LTs log normal. Total cysteinyl-LT levels, of which on average 75% was LTE4, were nearly 10 times higher than in earlier studies. Log LTE4 and total cysteinyl-LT levels correlated with the overall severity of pulmonary disease assessed by Chrispin-Norman chest radiograph score (Log LTE4: r = 0.701, r2 = 49.1%, P = 0.008. Log total cysteinyl-LTs: r = 0.715, r2 = 51.1%, P = 0.006). There was no apparent relationship between LTB4 levels and Chrispin-Norman chest radiograph score, nor between the level of any of the LTs and age or organism cultured from sputum. These findings suggest that the cysteinyl-LTs may be involved in the pathophysiology of pulmonary disease in CF.
Spencer
D A
DA
Department of Paediatrics, King's College Hospital, Denmark Hill, London, U.K.
Sampson
A P
AP
Green
C P
CP
Costello
J F
JF
Piper
P J
PJ
Price
J F
JF
D002648:Child; D002851:Chromatography, High Pressure Liquid; D003550:Cystic Fibrosis / Q000150:complications / Q000378:metabolism*; D006801:Humans; D017999:Leukotriene E4; D015289:Leukotrienes / Q000032:analysis*; D008168:Lung / Q000000981:diagnostic imaging*; D011859:Radiography; D011863:Radioimmunoassay; D012141:Respiratory Tract Infections / Q000000981:diagnostic imaging*; D013189:SRS-A / Q000031:analogs & derivatives / Q000032:analysis; D013183:Sputum / Q000737:chemistry*
D015289:Leukotrienes; D013189:SRS-A; D017999:Leukotriene E4