title: Differential roles of Fc gamma RII and Fc gamma RIII in immune complex stimulation of human neutrophils.
abstract: Insoluble immune complexes (IIC) stimulate human neutrophils through Fc gamma receptors. Freshly isolated human neutrophils express two FcR subclasses, FcRII and FcRIII. We explored the role of FcRII and FcRIII in this activation process by selectively binding each FcR subclass with the Fab fragments of the respective anti-FcR monoclonal antibodies (MFab) before exposure to IIC. Correlation among liganded FcR subclass, IIC binding, and ensuant IIC stimulation was achieved with multiparameter flow cytometry. We utilized rhodamine-labeled anti-FcRIII and fluorescein-labeled IIC to study binding and observed the change in [Ca2+]i in the same cell with a Ca2+ indicator, Indo-1. Treatment with either anti-FcRII (IV.3) or anti-FcRIII (3G8) MFab decreased both the fraction of cells exhibiting a Ca2+ transient and the magnitude of that transient, although only anti-FcRIII but not anti-FcRII significantly inhibited the subsequent IIC binding. In addition, cells treated with anti-FcRII and then stimulated with IIC exhibited a decrease in both the intracellular Ca2+ transient and the later Ca2+ influx, whereas anti-FcRIII totally abolished the mobilization of intracellular Ca2+ without affecting the Ca2+ influx. Treatment with either anti-FcR MFab decreased the IIC-stimulated transmembrane potential change, oxidative burst, and elastase release. These studies indicate that freshly isolated neutrophils' Fc receptor subclasses have unique roles in the IIC-initiated stimulation and that full activation can only be achieved when both FcR subclasses are available.
authors.affiliation: Department of Biochemistry, Boston University School of Medicine, Massachusetts 02118.
authors.forename: B A
authors.initials: BA
authors.lastname: Brunkhorst
authors.forename: G
authors.initials: G
authors.lastname: Strohmeier
authors.forename: K
authors.initials: K
authors.lastname: Lazzari
authors.forename: G
authors.initials: G
authors.lastname: Weil
authors.forename: D
authors.initials: D
authors.lastname: Melnick
authors.forename: H B
authors.initials: HB
authors.lastname: Fleit
authors.forename: E R
authors.initials: ER
authors.lastname: Simons
mesh_terms: D000911:Antibodies, Monoclonal / Q000502:physiology; D000936:Antigen-Antibody Complex / Q000502:physiology*; D002118:Calcium / Q000097:blood; D004533:Egtazic Acid / Q000494:pharmacology; D006801:Humans; D007074:Immunoglobulin G / Q000502:physiology; D007700:Kinetics; D019272:Leukocyte Elastase; D009504:Neutrophils / Q000187:drug effects / Q000276:immunology / Q000502:physiology*; D010196:Pancreatic Elastase / Q000378:metabolism; D017452:Receptors, IgG / Q000145:classification / Q000502:physiology*; D013481:Superoxides / Q000097:blood
chemical_list: D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D007074:Immunoglobulin G; D017452:Receptors, IgG; D013481:Superoxides; D004533:Egtazic Acid; D010196:Pancreatic Elastase; D019272:Leukocyte Elastase; D002118:Calcium