Simultaneous quantitation of delta-9-tetrahydrocannabinol (THC) and 11-nor-9-carboxy-delta-9-tetrahydrocannabinol (THC-COOH) in serum by GC/MS using deuterated internal standards and its application to a smoking study and forensic cases.
A new procedure for the simultaneous detection of delta-9-tetrahydrocannabinol (THC) and its major metabolite, 11-nor-9-carboxy-delta-9-tetrahydrocannabinol (THC-COOH) in serum has been evaluated. The method combines rapid, efficient, solid-phase extraction and simple derivatization by methylation. Analysis and quantitation is performed by gas chromatography/mass spectrometry (GC/MS) using deuterated cannabinoids as internal standards (IS). Reproducibility and sensitivity of the method are good. The procedure is applied to serum specimens collected from a smoking study with 24 volunteers and 212 forensic cases. Results are interpreted based upon the current knowledge about THC metabolism and pharmacokinetics.
Moeller
M R
MR
Institute of Legal Medicine, State University of Saarland, Homburg, Germany.
Doerr
G
G
Warth
S
S
D002138:Calibration; D013759:Dronabinol / Q000031:analogs & derivatives* / Q000097:blood* / Q000493:pharmacokinetics; D005260:Female; D005554:Forensic Medicine / Q000295:instrumentation / Q000379:methods*; D008401:Gas Chromatography-Mass Spectrometry; D006207:Half-Life; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D008385:Marijuana Smoking / Q000097:blood*; D015203:Reproducibility of Results
C016780:11-nor-delta(9)-tetrahydrocannabinol-9-carboxylic acid; D013759:Dronabinol