title: Nasal mucosa sensitization with toluene diisocyanate (TDI) increases preprotachykinin A (PPTA) and preproCGRP mRNAs in guinea pig trigeminal ganglion neurons.
abstract: Toluene diisocyanate (TDI) induces respiratory allergy in mammals. Using immunohistochemistry and in situ hybridization histochemistry, the present study examined effects of nasal mucosa sensitization by TDI on the immunoreactivity for substance P (SP) and calcitonin gene-related peptide (CGRP) and on the expression of their mRNAs in guinea pig trigeminal ganglion and their terminals. Single intranasal application of TDI (acute experiment) did not induce nasal allergy-like behaviours and failed to cause changes of SP and CGRP immunoreactivity and in the expression of preprotachykinin A (PPTA) mRNA and preproCGRP mRNA coding for SP and CGRP respectively in the trigeminal ganglion neurons. However, repeated application of TDI (chronic experiment) caused a dramatic increase of SP and CGRP immunoreactivity in peripheral neurites of sensory nerves in the nasal mucosa but a slight increase in the spinal trigeminal nucleus, a decrease of the same immunoreactivities in the cell bodies of the trigeminal ganglion neurons, and an increase of the expression of PPTA and preproCGRP mRNA in the same neurons. These findings suggest that chronic exposure of the nasal mucosa to TDI apparently causes enhancement of both the biosynthesis of SP and CGRP and their axonal transport in the trigeminal system.
authors.affiliation: Department of Otolaryngology, Osaka University Medical School, Japan.
authors.forename: B
authors.initials: B
authors.lastname: Kalubi
authors.forename: N
authors.initials: N
authors.lastname: Takeda
authors.forename: M
authors.initials: M
authors.lastname: Irifune
authors.forename: S
authors.initials: S
authors.lastname: Ogino
authors.forename: Y
authors.initials: Y
authors.lastname: Abe
authors.forename: S L
authors.initials: SL
authors.lastname: Hong
authors.forename: M
authors.initials: M
authors.lastname: Yamano
authors.forename: T
authors.initials: T
authors.lastname: Matsunaga
authors.forename: M
authors.initials: M
authors.lastname: Tohyama
mesh_terms: D000818:Animals; D001345:Autoradiography; D015740:Calcitonin Gene-Related Peptide / Q000235:genetics*; D015870:Gene Expression / Q000187:drug effects; D006168:Guinea Pigs; D006967:Hypersensitivity; D007114:Immunization; D009297:Nasal Mucosa / Q000187:drug effects / Q000502:physiology*; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D011498:Protein Precursors / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D012016:Reference Values; D013462:Sulfur Radioisotopes; D015320:Tachykinins / Q000235:genetics*; D014051:Toluene 2,4-Diisocyanate / Q000494:pharmacology*; D012668:Trigeminal Ganglion / Q000187:drug effects / Q000502:physiology*
chemical_list: D011498:Protein Precursors; D012333:RNA, Messenger; D013462:Sulfur Radioisotopes; D015320:Tachykinins; C076404:preprocalcitonin gene-related peptide; C044206:preprotachykinin; D014051:Toluene 2,4-Diisocyanate; D015740:Calcitonin Gene-Related Peptide