[Variants of the interaction of the human T-cell leukemia virus (HTLV-I) with the B-cells of lymphoblastoid lines].
B-cell lymphoblastoid lines which are known to be derived by in vitro inoculation of B-lymphocytes with Epstein-Barr herpes virus (EBV) were shown to be infected with HTLV-1. Three possible variants of HTLV-1 interaction with cells were demonstrated by immunoblot, polymerase chain reaction, and virus isolation: (1) prolonged productive infection; (2) infection of the cells manifested only by the presence of "silent" virus sequences; (3) temporary production of HTLV-1 (3.5 months) after the end of which genetic material persisted in the cells. The long-term productive HTLV-1 infection in EBV-infected B-cells was found to influence the functioning of EBV genome which was manifested by expression of two additional proteins of EBNA-5 group and by changes in the intensity and pattern of LMP and EBNA-2 proteins the functioning of which is associated with immortalizing and transforming properties of EBV.
Iakovleva
L S
LS
Pavlish
O A
OA
Tupitsin
N N
NN
Gurtsevich
V E
VE
D001402:B-Lymphocytes / Q000382:microbiology*; D002461:Cell Line, Transformed; D005434:Flow Cytometry; D004854:Herpesvirus 4, Human / Q000378:metabolism / Q000472:pathogenicity; D015368:Human T-lymphotropic virus 1 / Q000378:metabolism / Q000472:pathogenicity*; D006801:Humans; D015151:Immunoblotting / Q000379:methods; D016133:Polymerase Chain Reaction / Q000379:methods; D013997:Time Factors; D014764:Viral Proteins / Q000032:analysis / Q000096:biosynthesis; D014776:Virus Cultivation
D014764:Viral Proteins
Varianty vzaimodeĭstviia virusa T-kletochnogo leĭkoza cheloveka (HTLV-I) s B-kletkami limfoblastoidnykh liniĭ.