Detection of human papilloma virus types 16 & 18 DNA in cervical lesions of Indian women using in situ hybridization.
Fifty one biopsies from women with malignant lesions of the uterine cervix and 9 biopsies fron non-malignant lesions were examined for the presence of HPV 16 and 18 DNA sequences by in situ hybridization method using 35S-labelled DNA probes. HPV 16 DNA sequences were detected in 82.4 per cent biopsies from women with malignant lesions, whereas HPV 18 DNA was detected in only 3 biopsies which were also positive for HPV 16 DNA. Two biopsies from non-malignant lesions were positive for HPV 16 DNA only. Data were also analysed according to the histologic type of cancer. It was observed that no significant correlation existed between HPV types and different histologic types of cervical cancer.
Sarkar
S
S
Department of Microbiology, All India Institute of Medical Sciences, New Delhi.
Verma
K
K
Kaur
H
H
Seth
P
P
D015344:DNA Probes, HPV; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D006801:Humans; D017403:In Situ Hybridization; D007194:India; D027383:Papillomaviridae / Q000302:isolation & purification*; D014412:Tumor Virus Infections / Q000175:diagnosis*; D002577:Uterine Cervical Diseases / Q000382:microbiology*
D015344:DNA Probes, HPV; D004279:DNA, Viral