title: Assignment of the redox potentials to the four haems in Desulfovibrio vulgaris cytochrome c3 by 2D-NMR.
abstract: Using 2D-NMR the four haems of Desulfovibrio vulgaris (Hildenborough) cytochrome c3 within the X-ray structure were fully cross assigned according to their redox potential. The strategy used was based on a complete network of chemical exchange connectivities between the NMR signals obtained for all oxidation levels to the corresponding ones in the fully reduced spectrum [1992, Eur. J. Biochem., in press]. This unequivocal cross-assignment disagrees with earlier results obtained for the similar protein from Desulfovibrio vulgaris (Miyazaki F.).
authors.affiliation: Centro de Tecnologia Química e Biológica, Oeiras, Portugal.
authors.forename: C A
authors.initials: CA
authors.lastname: Salgueiro
authors.forename: D L
authors.initials: DL
authors.lastname: Turner
authors.forename: H
authors.initials: H
authors.lastname: Santos
authors.forename: J
authors.initials: J
authors.lastname: LeGall
authors.forename: A V
authors.initials: AV
authors.lastname: Xavier
mesh_terms: D003574:Cytochrome c Group / Q000737:chemistry*; D016969:Desulfovibrio vulgaris / Q000737:chemistry*; D006418:Heme / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D010084:Oxidation-Reduction; D013329:Structure-Activity Relationship; D014961:X-Ray Diffraction
chemical_list: D003574:Cytochrome c Group; D006418:Heme; C017165:cytochrome c(3)