title: Mobilization of nonconjugative pBR322-derivative plasmids from laboratory strains of Escherichia coli to bacteria isolated from seawater.
abstract: Mobilization of derivatives of plasmids pBR322 and pBR325 was shown to occur between Escherichia coli K12 strains in LB-broth at 37 degrees C, provided a mobilizer plasmid (F') was present either together with the nonconjugative plasmid or in a second donor strain. Evidence from restriction endonuclease analysis suggested that the mobilization was facilitated by a transposition phenomenon involving the "gamma-delta" sequence of F'. It was shown that mobilization of a derivative of pBR325 from E. coli K12 to bacteria isolated from seawater occurred in incubations in both LB-broth and filtered seawater and that Pseudomonas sp., Enterobacter aerogenes, Klebsiella oxytoca, E. coli, and Citrobacter amalonaticus isolates were recipient-active.
authors.affiliation: Department of General Microbiology, University of Copenhagen, Denmark.
authors.forename: S J
authors.initials: SJ
authors.lastname: Sørensen
mesh_terms: D002954:Citrobacter / Q000235:genetics; D003227:Conjugation, Genetic; D004269:DNA, Bacterial / Q000032:analysis / Q000302:isolation & purification; D004587:Electrophoresis, Agar Gel; D004754:Enterobacter / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D015151:Immunoblotting; D007709:Klebsiella / Q000235:genetics; D010957:Plasmids / Q000235:genetics*; D011549:Pseudomonas / Q000235:genetics; D012623:Seawater; D014169:Transformation, Bacterial / Q000502:physiology*; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial