title: Characterization of a plasma retinol-binding protein membrane receptor expressed in the retinal pigment epithelium.
abstract: A specific membrane receptor for plasma retinol-binding protein (RBP) is expressed in the retinal pigment epithelium (RPE). When chemically cross-linking RBP to RPE membranes, an 86-kDa RBP.RBP receptor complex is formed, and a 63-kDa protein was identified as the RBP-binding membrane protein (Båvik, C.-O., Eriksson, U., Allen, R., and Peterson, P. (1991) J. Biol. Chem. 266, 14978-14985). To explore in more detail the characteristics of this membrane receptor, we have generated a monoclonal antibody, A52, to the 63-kDa protein (p63). A52 binds the 86-kDa RBP.RBP receptor complex and p63. Several lines of evidence suggest that p63 is not a regular integral membrane protein, and it occurs in different forms. One form is firmly attached to membranes, is part of a high molecular weight complex, and is able to bind RBP. The other form of p63 can be removed from membranes by treatment with an alkaline buffer and is unable to bind RBP. Both forms of p63 contain extensive hydrophobic domains and are found in the detergent phase upon extraction with Triton X-114. The expression of p63 is restricted to RPE, and immunohistochemical localization of tissue sections from bovine retina showed highest expression in the basolateral portion of RPE cells. Immunofluorescence localization, using isolated RPE cells, showed that p63 is exposed on the cell surface of newly isolated RPE cells.
authors.affiliation: Ludwig Institute for Cancer Research, Stockholm, Sweden.
authors.forename: C O
authors.initials: CO
authors.lastname: Båvik
authors.forename: C
authors.initials: C
authors.lastname: Busch
authors.forename: U
authors.initials: U
authors.lastname: Eriksson
mesh_terms: D000818:Animals; D001665:Binding Sites; D002417:Cattle; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D005455:Fluorescent Antibody Technique; D008565:Membrane Proteins / Q000302:isolation & purification / Q000378:metabolism; D008970:Molecular Weight; D010857:Pigment Epithelium of Eye / Q000166:cytology / Q000378:metabolism*; D011485:Protein Binding; D011956:Receptors, Cell Surface / Q000302:isolation & purification / Q000378:metabolism*; D012160:Retina / Q000166:cytology / Q000378:metabolism; D012177:Retinol-Binding Proteins / Q000302:isolation & purification / Q000378:metabolism*; D054839:Retinol-Binding Proteins, Plasma
chemical_list: D008565:Membrane Proteins; D011956:Receptors, Cell Surface; D012177:Retinol-Binding Proteins; D054839:Retinol-Binding Proteins, Plasma; C027928:retinol binding protein receptor