Mineralocorticoid hormone action in plant cells.
The multiplication of Chlamydomonas reinhardtii wild type cells can be arrested by the spirolactone RU 26752 and this is fully reversible by the natural mineralocorticoid aldosterone. Evidence is presented for a 52 kDa protein that possesses functional DNA and ligand binding domains and tests positive for mineralocorticoid receptor-like activity by immuneprecipitation, macroaggregation, and photoaffinity. The regulation of trans-activation by steroid hormones in the animal world would therefore appear to be just as valid for the plant kingdom, thereby providing a new model for genetic analysis.
Mirshahi
M
M
INSERM U-86, Centre Universitaire des Cordeliers, Paris, France.
Mirshahi
A
A
Nato
A
A
Agarwal
M K
MK
D000450:Aldosterone / Q000494:pharmacology*; D000818:Animals; D016825:Chlamydomonas reinhardtii / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism; D002852:Chromatography, Ion Exchange; D003600:Cytosol / Q000187:drug effects / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000302:isolation & purification / Q000378:metabolism*; D007074:Immunoglobulin G / Q000302:isolation & purification; D008970:Molecular Weight; D018161:Receptors, Mineralocorticoid; D011987:Receptors, Steroid / Q000187:drug effects / Q000302:isolation & purification / Q000378:metabolism*; D013148:Spironolactone / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology
D004268:DNA-Binding Proteins; D007074:Immunoglobulin G; D018161:Receptors, Mineralocorticoid; D011987:Receptors, Steroid; D013148:Spironolactone; D000450:Aldosterone; C047175:7-propyl spirolactone