Effect of the terminal phosphate derivatization of beta- and alpha-oligodeoxynucleotides on their antisense activity in protein biosynthesis, stability and uptake by eucaryotic cells.
Inhibition of polypeptide chain elongation with the mRNA-complementary (antisense) oligonucleotide has been realized through a RNase H independent mechanism. Nuclease resistant complementary non-natural alpha-17-mer oligonucleotide did not inhibit cell-free protein biosynthesis of beta-globin in the wheat germ system because it did not elicit RNase H activity. Linkage of alkylating group [4-(N-2-chloroethyl-N-methyl)-aminobenzyl]-methylamine to the 5'-terminus of the alpha-oligomer led to the formation of its covalent adduct with mRNA which could not be translated in vitro. Linkage of hydrophobic residues to the terminal phosphates of natural oligonucleotides increased their stability against nucleases and uptake by human cancer cells. A porphyrin, substituted in the meso-position by aromatic groups, gave a rise to an approximately six-fold increase of uptake and cholesterol a 30-100-fold increase. Eighty percent of bound derivatives were found in cytoplasmic cellular fractions.
Boutorine
A S
AS
Institute of Bioorganic Chemistry, Siberian Division, Russian Academy of Sciences, Novosibirsk.
Boiziau
C
C
Le Doan
T
T
Toulmé
J J
JJ
Hélène
C
C
D000478:Alkylation; D001483:Base Sequence; D002784:Cholesterol / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005914:Globins / Q000096:biosynthesis*; D006801:Humans; D008969:Molecular Sequence Data; D016376:Oligonucleotides, Antisense / Q000737:chemistry / Q000378:metabolism*; D010710:Phosphates / Q000378:metabolism; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism*; D016914:Ribonuclease H / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D016376:Oligonucleotides, Antisense; D010710:Phosphates; D012333:RNA, Messenger; D005914:Globins; D002784:Cholesterol; D016914:Ribonuclease H