A gene homologous to topoisomerase II in African swine fever virus.
A putative topoisomerase II gene of African swine fever virus was mapped using a degenerate oligonucleotide probe derived from a region highly conserved in type II topoisomerases. The gene is located within EcoRI fragments P and H of the African swine fever virus genome. Sequencing of this region has revealed a long open reading frame, designated P1192R, encoding a protein of 1192 amino acids, with a predicted molecular weight of 135,543. Open reading frame P1192R is transcribed late after infection into a 4.6-kb RNA. The deduced amino acid sequence of this open reading frame shares significant similarity with topoisomerase II sequences from different sources, with percentages of identity between 23 and 29%. The evolutionary relationships among the topoisomerase II sequences of ASF virus, eukaryotes and prokaryotes were analyzed and a phylogenetic tree was established. The tree indicates that the ASF virus topoisomerase II gene was present in the virus genome before protozoa, yeasts, and metazoa diverged.
García-Beato
R
R
Centro de Biología Molecular, (CSIC-UAM), Facultad de Ciencias, Universidad Autónoma, Madrid, Spain.
Freije
J M
JM
López-Otín
C
C
Blasco
R
R
Viñuela
E
E
Salas
M L
ML
D000358:African Swine Fever Virus / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005075:Biological Evolution; D004250:DNA Topoisomerases, Type II / Q000235:genetics*; D004279:DNA, Viral / Q000235:genetics; D015870:Gene Expression; D005814:Genes, Viral; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D012333:RNA, Messenger / Q000235:genetics; D015183:Restriction Mapping; D016415:Sequence Alignment; D014709:Vero Cells; D015678:Viral Structural Proteins / Q000235:genetics*
D004279:DNA, Viral; D009838:Oligodeoxyribonucleotides; D012333:RNA, Messenger; D015678:Viral Structural Proteins; D004250:DNA Topoisomerases, Type II