title: Polyclonal and monoclonal antibodies monospecific to MMTV LTR orf protein produced in E. coli.
abstract: Monoclonal and polyclonal antibodies specific to an open reading frame of the mouse mammary tumor virus long terminal repeat were generated using an open reading frame-beta-galactosidase fusion protein produced in E. coli. Both antibodies reacted with the open reading frame-beta-galactosidase fusion protein but not with beta-galactosidase alone using an immunoblotting technique. It is concluded that these antibodies were specific for the protein encoded by the open reading frame of the mouse mammary tumor virus long terminal repeat.
authors.affiliation: Second Department of Anatomy, Nara Medical University, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Haga
authors.forename: K
authors.initials: K
authors.lastname: Shigesada
authors.forename: Y
authors.initials: Y
authors.lastname: Namba
authors.forename: H
authors.initials: H
authors.lastname: Tanaka
authors.forename: S
authors.initials: S
authors.lastname: Imai
authors.forename: J
authors.initials: J
authors.lastname: Morimoto
authors.forename: S
authors.initials: S
authors.lastname: Hiroishi
authors.forename: H
authors.initials: H
authors.lastname: Yamamoto
authors.forename: N H
authors.initials: NH
authors.lastname: Sarkar
authors.forename: J
authors.initials: J
authors.lastname: Hilgers
mesh_terms: D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D000914:Antibodies, Viral / Q000096:biosynthesis*; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D006046:Gold; D007150:Immunohistochemistry; D008324:Mammary Tumor Virus, Mouse / Q000201:enzymology / Q000235:genetics*; D015514:Oncogene Proteins, Fusion / Q000235:genetics* / Q000276:immunology* / Q000302:isolation & purification; D016366:Open Reading Frames; D010957:Plasmids; D012091:Repetitive Sequences, Nucleic Acid; D001616:beta-Galactosidase / Q000235:genetics* / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D015514:Oncogene Proteins, Fusion; D006046:Gold; D001616:beta-Galactosidase