title: Mapping of a human brain voltage-gated calcium channel to human chromosome 12p13-pter.
abstract: Degenerate DNA oligomers coding for highly conserved regions of the voltage-gated calcium channel were synthesized for the polymerase chain reaction (PCR) using DNA from a human brain cDNA library as template. PCR amplified a 640-bp DNA fragment from the human brain cDNA library. Sequencing revealed that this fragment encodes part of a protein highly homologous to a subtype of the dihydropyridine-sensitive calcium channel cloned from rabbit heart and rat brain. Southern analysis of panels of somatic cell hybrids mapped the 640-bp fragment, CACNL1A1, to human chromosome 12p13-pter.
authors.affiliation: Department of Biology, University of California San Diego, La Jolla 92093-0319.
authors.forename: W
authors.initials: W
authors.lastname: Sun
authors.forename: J D
authors.initials: JD
authors.lastname: McPherson
authors.forename: D Q
authors.initials: DQ
authors.lastname: Hoang
authors.forename: J J
authors.initials: JJ
authors.lastname: Wasmuth
authors.forename: G A
authors.initials: GA
authors.lastname: Evans
authors.forename: M
authors.initials: M
authors.lastname: Montal
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D001921:Brain / Q000378:metabolism*; D015220:Calcium Channels / Q000235:genetics*; D020746:Calcium Channels, L-Type; D002874:Chromosome Mapping; D002881:Chromosomes, Human, Pair 12; D006224:Cricetinae; D004247:DNA; D006801:Humans; D006822:Hybrid Cells; D015640:Ion Channel Gating; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics*; D016133:Polymerase Chain Reaction
chemical_list: C079146:CACNA1C protein, human; D015220:Calcium Channels; D020746:Calcium Channels, L-Type; D009419:Nerve Tissue Proteins; D004247:DNA