[Laboratory study and clinical evaluation of ABC-ELISA for detection of mycoplasma pneumoniae antigens].
Using anti-M. pneumoniae IgG obtained by immunizing rabbits with whole cell antigen of M. pneumoniae strain FH, we established an ABC-ELISA for detection of M. pneumoniae in simulated positive specimens, ABC-ELISA had a detection limit of 10(4) cfu/ml of specimen. There were no cross-reaction with 4 other species of human mycoplasmas and 12 common species of bacteria in respiratory tract. In 47 patients suspected of being M. pneumoniae infection, we compared the results of ABC-ELISA with the combined results from serodiagnostic methods and M. pneumoniae culture. The coincident rate between them was 80.9%.
Yu
K L
KL
Changzheng Hospital, Shanghai.
D000942:Antigens, Bacterial / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D009177:Mycoplasma pneumoniae / Q000302:isolation & purification*; D010614:Pharynx / Q000382:microbiology; D011019:Pneumonia, Mycoplasma / Q000175:diagnosis*
D000942:Antigens, Bacterial