title: Intranuclear inclusions in paramyxovirus-induced encephalitis: evidence for altered nuclear body differentiation.
abstract: Intranuclear inclusion bodies (INB) are frequently encountered in viral infections, where they are thought to be accumulations of viral particles. However, for RNA viruses replicating in the cytoplasm, this compartmentalization represents a paradox not consistent with the viral replication cycle. To define the basis for intranuclear paramyxoviral inclusion bodies in astrocytes, natural cases of canine distemper virus subacute encephalitis were examined by light and transmission electron microscopy, and by quantitative confocal immunofluorescence microscopy. Although INB were viral antigen positive, they were not composed of structurally recognizable paramyxoviral nucleocapsids. The structural basis for the INB was instead viral antigen-associated forms of nucleolar development known as nuclear bodies. Three variants of the light microscopic Cowdry type A INB were complex nuclear bodies, giant beaded nuclear bodies (sphaeridia), and nuclear body-associated granulofilamentous matrices. In the latter, the granulofilamentous matrix frequently filled the nucleus, resulting in a fourth morphological INB variant, and was associated with morphological evidence of nuclear degeneration. These findings suggest a novel mechanism of virus-induced cytopathology whereby intranuclear viral protein exerts deleterious effects upon nucleolar differentiation in infected cells and hence altered host cell RNA metabolism.
authors.affiliation: Department of Veterinary Pathobiology, Ohio State University, Columbus 43210.
authors.forename: M
authors.initials: M
authors.lastname: Oglesbee
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000648:ultrastructure; D002467:Cell Nucleus / Q000648:ultrastructure*; D004216:Distemper / Q000473:pathology*; D004217:Distemper Virus, Canine / Q000648:ultrastructure*; D004285:Dogs; D002479:Inclusion Bodies / Q000648:ultrastructure*; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence