ESR spin trapping analysis of gamma induced radicals in sucrose: II.
Radicals induced by gamma-irradiation of sucrose, in the solid state at different temperatures and in aqueous solution, have been investigated by the spin trapping method. Electron spin resonance (ESR) combined with high performance liquid chromatography (HPLC), followed by spectral analysis with a simulation program (Voyons) revealed seven main radical species. A comparative study of the ESR signals from spin trapped gamma-induced radicals in some glycosides, disaccharides, 13C specifically labelled carbohydrates, as well as in several deoxysucroses and fructans, led to the assignment of a chemical structure to five out of the seven sucrose-nitroxide adducts previously evidenced. Sucrose is shown to be a conceivable model for the study of fructans gamma-radiolysis mechanism in aqueous solution.
Triolet
J
J
Groupe de Radiochimie du Stress Oxydatif, DPVE/SIV, Centre de Cadarache, Saint-Paul-lez-Durance, France.
Thiery
C
C
Agnel
J P
JP
Battesti
C
C
Raffi
J
J
Vincent
P
P
D002240:Carbohydrate Sequence; D004578:Electron Spin Resonance Spectroscopy; D005609:Free Radicals / Q000737:chemistry; D005720:Gamma Rays; D008969:Molecular Sequence Data; D015394:Molecular Structure; D013113:Spin Labels; D013395:Sucrose / Q000737:chemistry / Q000528:radiation effects*
D005609:Free Radicals; D013113:Spin Labels; D013395:Sucrose