Interaction of various Entamoeba histolytica strains with human intestinal cell lines.
The interaction of four pathogenic and three nonpathogenic E. histolytica strains with two human intestinal cell lines (Caco-2 and HT-29) was examined. The adherence of pathogenic and nonpathogenic E. histolytica to these cells was similar, indicating that defective adherence to intestinal cells is not a common feature of nonpathogenic strains. The addition of different carbohydrates confirmed the importance of the galactose-binding lectin of E. histolytica in binding to these intestinal cells. On the other hand, only virulent E. histolytica strains damaged monolayers of intestinal cells. The results indicate that Caco-2 cells and differentiated HT-29 cells are useful models for research of intestinal amebiasis.
Burchard
G D
GD
Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany.
Prange
G
G
Mirelman
D
D
D000818:Animals; D002277:Carcinoma / Q000473:pathology; D002448:Cell Adhesion; D002454:Cell Differentiation; D003106:Colon / Q000473:pathology*; D003110:Colonic Neoplasms / Q000473:pathology; D004748:Entamoeba histolytica / Q000472:pathogenicity*; D006801:Humans; D013045:Species Specificity; D014407:Tumor Cells, Cultured; D014774:Virulence