title: Possible role of surface potential in the gating mechanism of Ca2+ channels in cat adrenal chromaffin cells: studies with fura-2 microfluorometry.
abstract: The cytosolic free Ca2+ concentration ([Ca]in) in isolated cat chromaffin cells was measured by fura-2 microfluorometry. During 30 mM KCl depolarization or sucrose substitution for NaCl, a reduction in external Ca2+ concentration under optimal conditions paradoxically caused a rise in [Ca]in and, in separate experiments, in catecholamine secretion. The results support a previously suggested role of surface potentials in the gating mechanism of Ca2+ channels.
authors.affiliation: Department of Physiology, Kagashima University School of Medicine, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Sorimachi
authors.forename: S
authors.initials: S
authors.lastname: Nishimura
authors.forename: K
authors.initials: K
authors.lastname: Yamagami
mesh_terms: D000313:Adrenal Medulla / Q000166:cytology*; D000818:Animals; D002118:Calcium / Q000378:metabolism; D015220:Calcium Channels / Q000502:physiology*; D002415:Cats; D003592:Cytophotometry; D016257:Fura-2; D066298:In Vitro Techniques; D015640:Ion Channel Gating / Q000502:physiology; D008564:Membrane Potentials / Q000502:physiology
chemical_list: D015220:Calcium Channels; D002118:Calcium; D016257:Fura-2