[Effects of aluminum, fluorine and their mixtures on the enzyme activities of jejunal mucus membrane in the perfusion of small intestine of rats in vivo].
Buffer (control), F as NaF (100 mmol/L), Al as AlCl3 (32 and 170 mmol/L) and the two mixtures of Al and F (Al:F = 0.3:0.7 and 0.7:0.3) in Tris-buffered media of pH 7.0 were respectively perfused through the small intestine of rats over a period of 40 min. The jejunum was taken out from each animal immediately. Some of its parts were for HE staining; and the others for enzyme histochemical staining. Fifteen enzymes were observed. The results showed that (1) Fluorine chiefly inhibited the metal-enzymes and metal-activated enzymes; aluminum mainly depressed the activities of enzymes in relation to the cell energy metabolism, such as hydrogenases and oxidase. (2) The decreased activities of enzymes induced by Al or F increased significantly after mixing with each other, indicating that the simultaneous existence of Al and F decreased their ionic concentrations, so that strong antagonistic effects on the inhibition of enzymes were noticed since F or Al inhibited enzyme activities by their ions. (3) A high dose of Al intensively inhibited the enzymes related to the cell energy metabolism, which explained why less quantity of Al was absorbed in animals with high dose of Al than that of low dose Al seen in the absorption experiment. (4) The enzyme activities in the animals given high dose of Al mixed with F apparently were elevated in comparison with that in the animals given a pure high dose of Al which was the result of antagonistic effects of F on the inhibition of enzyme activities by Al, therefore the quantity of the absorbed Al was high.
Xiao
B
B
Li
D
D
Dong
Q
Q
Li
Q
Q
Li
S
S
Zhan
C
C
D000535:Aluminum / Q000494:pharmacology*; D000818:Animals; D003576:Electron Transport Complex IV / Q000378:metabolism; D005461:Fluorine / Q000494:pharmacology*; D006867:Hydrolases / Q000378:metabolism; D007413:Intestinal Mucosa / Q000201:enzymology; D007583:Jejunum / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D010088:Oxidoreductases / Q000378:metabolism*; D010477:Perfusion; D051381:Rats; D011919:Rats, Inbred Strains
D005461:Fluorine; D000535:Aluminum; D010088:Oxidoreductases; D003576:Electron Transport Complex IV; D006867:Hydrolases