Regulation of flagellar motility by temperature-dependent phosphorylation of a 43 kDa axonemal protein in fowl spermatozoa.
Phosphorylation of fowl sperm proteins was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) after incubating the demembranated spermatozoa with [gamma-32P]ATP at 30 degrees C or 40 degrees C. A marked difference of phosphorylation between 30 degrees C and 40 degrees C was observed in a 43 kDa protein. This protein was slightly phosphorylated at 40 degrees C, but strongly phosphorylated at 30 degrees C in a cAMP-independent manner. The motility of demembranated spermatozoa was negligible at 40 degrees C, but vigorous movement was observed at 30 degrees C. These results showed that phosphorylation of a 43 kDa protein is likely to be a regulatory step in the maintenance of fowl sperm motility.
Ashizawa
K
K
Laboratory of Animal Reproduction, Faculty of Agriculture, Miyazaki University, Japan.
Katayama
S
S
Tsuzuki
Y
Y
D000818:Animals; D002645:Chickens; D000242:Cyclic AMP / Q000378:metabolism; D003598:Cytoskeletal Proteins / Q000737:chemistry / Q000378:metabolism; D066298:In Vitro Techniques; D008297:Male; D008970:Molecular Weight; D010750:Phosphoproteins / Q000378:metabolism; D010766:Phosphorylation; D013081:Sperm Motility; D013082:Sperm Tail / Q000502:physiology*; D013696:Temperature
D003598:Cytoskeletal Proteins; D010750:Phosphoproteins; D000242:Cyclic AMP