Initial characterization of whole-cell currents from freshly dissociated corneal keratocytes.
The perforated patch technique was utilized to obtain whole-cell currents from freshly dissociated rabbit corneal keratocytes. We describe and provide the initial characterization of two distinct whole cell currents in rabbit keratocytes: a K(+)-selective delayed rectifier and a voltage-sensitive, tetrodotoxin blockable Na+ current. The voltage-sensitive Na+ current is of sufficient magnitude to allow us to initiate action potentials when current-clamping the cells. This is the first detailed electrophysiological study of corneal keratocytes.
Watsky
M A
MA
Department of Physiology and Biophysics, Mayo Foundation, Rochester, MN 55905.
Rae
J L
JL
D000200:Action Potentials; D000818:Animals; D001692:Biological Transport; D002478:Cells, Cultured; D003319:Corneal Stroma / Q000166:cytology / Q000502:physiology*; D004594:Electrophysiology; D011188:Potassium / Q000378:metabolism; D015221:Potassium Channels / Q000502:physiology*; D011817:Rabbits; D012964:Sodium / Q000378:metabolism; D015222:Sodium Channels / Q000502:physiology*
D015221:Potassium Channels; D015222:Sodium Channels; D012964:Sodium; D011188:Potassium