title: Identification of sequence types among the M-nontypeable group A streptococci.
abstract: Streptococcal diseases, namely, acute glomerulonephritis and acute rheumatic fever, are common features in the aboriginal population of the Northern Territory of Australia. We examined the group A streptococcal M types identified during various surveys conducted since 1987. Streptococci were predominantly isolated from skin infections. A high proportion of the isolates could not be serotyped by conventional means and were designated M nontypeable (MNT). M-specific DNA sequences from the MNT isolates were examined, and sequence types were proposed for the classification of MNTs. This allowed a more precise estimate of the M types present in a population study.
authors.affiliation: Menzies School of Health Research, Casuarina, Australia.
authors.forename: W A
authors.initials: WA
authors.lastname: Relf
authors.forename: D R
authors.initials: DR
authors.lastname: Martin
authors.forename: K S
authors.initials: KS
authors.lastname: Sriprakash
mesh_terms: D000595:Amino Acid Sequence; D001315:Australia; D015373:Bacterial Typing Techniques; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012703:Serotyping; D013290:Streptococcal Infections / Q000382:microbiology; D013297:Streptococcus pyogenes / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification
chemical_list: D004269:DNA, Bacterial