High-performance liquid chromatography and preliminary pharmacokinetics of rufloxacin and its metabolites, N-desmethylrufloxacin and rufloxacinsulfoxide, in urine of rhesus monkey Macaca mulatta.
A gradient high-performance liquid chromatographic method for the quantification of rufloxacin and two of its metabolites in urine, N-desmethylrufloxacin and rufloxacinsulfoxide, has been developed and validated. Monkey urine samples were diluted ten times with distilled water and 20 microliters were injected onto a Cp Spher 5-ODS column, 5 microns particle size. The mobile phase was a mixture of 4% acetonitrile and 96% buffer at time 0, which changed linearly over 37 min to 26% acetonitrile and 74% buffer. Detection was achieved at 246 nm. The limit of detection of the three compounds was 0.50 microgram/ml. An example of a pharmacokinetic study of rufloxacin and its metabolites in monkeys is shown.
Vree
T B
TB
Department of Clinical Pharmacy, Academic Hospital Nijmegen Sint Radboud, Netherlands.
van den Biggelaar-Martea
M
M
Peeters
A
A
Imbimbo
B P
BP
D000818:Animals; D000890:Anti-Infective Agents; D002851:Chromatography, High Pressure Liquid; D024841:Fluoroquinolones; D008253:Macaca mulatta; D015363:Quinolones / Q000493:pharmacokinetics* / Q000652:urine; D013056:Spectrophotometry, Ultraviolet; D013454:Sulfoxides / Q000493:pharmacokinetics* / Q000652:urine; D013843:Thiazines / Q000493:pharmacokinetics* / Q000652:urine
D000890:Anti-Infective Agents; D024841:Fluoroquinolones; D015363:Quinolones; D013454:Sulfoxides; D013843:Thiazines; C074116:N-desmethylrufloxacin; C074117:rufloxacin sulfoxide; C060328:rufloxacin