Blotting and band-shifting: techniques for studying protein-protein interactions.
The type II cAMP-dependent protein kinase (PKA) is localized in certain cellular compartments through association with specific A-kinase anchoring proteins (AKAPs). A variety of blotting and electrophoresis techniques have been developed to study the protein-protein interactions that occur between the regulatory (R) subunit of PKA and AKAPs. These methods have also been used for a variety of purposes such as detecting calmodulin-binding proteins, comparing wild-type- and mutant-form binding affinities and estimating the molecular weight of multiprotein complexes.
Carr
D W
DW
Vollum Institute for Advanced Biomedical Research, Portland, OR 97201-3089.
Scott
J D
JD
D000818:Animals; D015153:Blotting, Western / Q000379:methods*; D002352:Carrier Proteins; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D010770:Phosphotransferases; D011493:Protein Kinase C / Q000378:metabolism; D011494:Protein Kinases / Q000378:metabolism*
D002352:Carrier Proteins; D010770:Phosphotransferases; D011494:Protein Kinases; D011493:Protein Kinase C