title: Ultrastructural localization of inositol 1,4,5-trisphosphate 3-kinase in rat cerebellar cortex.
abstract: Subcellular localization of inositol 1,4,5-trisphosphate 3-kinase in the rat cerebellar cortex was studied immunohistochemically using a monoclonal antibody. Electron microscopy revealed intense immunoreactivity in the dendritic spines of Purkinje cells forming synapses with the parallel fibers, climbing fibers and recurrent collaterals of Purkinje cell axons. The labelling was associated with the hypolemmal cisternae, surrounding matrix and plasmalemma including the postsynaptic densities. Weaker immunoreactivity was present in the dendritic spines of basket cells and in certain segments of Purkinje cell recurrent collaterals. The postsynaptic regions of the dendritic trunks of Purkinje and basket cells were negative. These results indicate that inositol 1,4,5-trisphosphate 3-kinase is distributed amongst the spines of various synaptic relations with different electrophysiological properties, and that axon terminals of certain cell types are another functional site for the enzyme.
authors.affiliation: Department of Pathology, Niigata University, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Yamada
authors.forename: A
authors.initials: A
authors.lastname: Kakita
authors.forename: M
authors.initials: M
authors.lastname: Mizuguchi
authors.forename: S G
authors.initials: SG
authors.lastname: Rhee
authors.forename: S U
authors.initials: SU
authors.lastname: Kim
authors.forename: F
authors.initials: F
authors.lastname: Ikuta
mesh_terms: D000818:Animals; D001369:Axons / Q000201:enzymology / Q000648:ultrastructure; D002525:Cerebellar Cortex / Q000201:enzymology* / Q000648:ultrastructure; D003712:Dendrites / Q000201:enzymology / Q000648:ultrastructure; D016253:Microscopy, Immunoelectron; D009412:Nerve Fibers / Q000201:enzymology / Q000648:ultrastructure; D010770:Phosphotransferases / Q000032:analysis*; D017853:Phosphotransferases (Alcohol Group Acceptor); D011689:Purkinje Cells / Q000201:enzymology* / Q000648:ultrastructure; D051381:Rats; D011919:Rats, Inbred Strains; D013569:Synapses / Q000201:enzymology* / Q000648:ultrastructure
chemical_list: D010770:Phosphotransferases; D017853:Phosphotransferases (Alcohol Group Acceptor); C051038:Inositol 1,4,5-trisphosphate 3-kinase