Differential effects of platelet-activating factor on superoxide anion production in human eosinophils and neutrophils.
The effect of platelet-activating factor (PAF) on the generation of superoxide anion (O2-) in human eosinophils and neutrophils was examined. The presence of PAF potentiated O2- production in either opsonized zymosan- or formyl-methionyl-leucyl-phenylalanine (FMLP)-stimulated cells. The effect of PAF was prominent in opsonized zymosan-stimulated eosinophils and in FMLP-stimulated neutrophils. We also found that eosinophils generate substantial amounts of O2- when treated with PAF alone. The high responsiveness of unstimulated or opsonized zymosan-stimulated eosinophils to PAF to generate O2- may be relevant to the pathological changes at the loci of allergic reactions where eosinophils and PAF are crucially involved.
Mabuchi
K
K
Faculty of Pharmaceutical Sciences, Teikyo University, Kanagawa, Japan.
Sugiura
T
T
Ojima-Uchiyama
A
A
Masuzawa
Y
Y
Waku
K
K
D004804:Eosinophils / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism; D010972:Platelet Activating Factor / Q000494:pharmacology*; D013481:Superoxides / Q000378:metabolism*; D015054:Zymosan / Q000494:pharmacology
D010972:Platelet Activating Factor; D013481:Superoxides; D009240:N-Formylmethionine Leucyl-Phenylalanine; D015054:Zymosan