Liquid chromatographic mass spectrometric methods for the determination of marine polyether toxins.
Experiments with novel nitrogenous coumarin-based reagents yielded moderately fluorescent derivatives of brevetoxin-3 and ciguatoxin-1. The diethylaminocoumarin-carbamic acid esters of brevetoxin-3 were resolved by high performance liquid chromatography into two derivative peaks that correspond to substitutions at the C-37 and C-41 hydroxyls. The derivatives produced intense molecular ions under fast atom bombardment ionization conditions, confirming derivative identity. Ciguatoxin-1 was also successfully derivatized, resolved, and identified by HPLC-fluorometry with an estimated lower limit of detection of 0.5 to 1.0 ng.
Dickey
R W
RW
US Food and Drug Administration, Division of Seafood Research, Dauphin Island, AL 36528.
Bencsath
F A
FA
Granade
H R
HR
Lewis
R J
RJ
D002851:Chromatography, High Pressure Liquid; D002922:Ciguatoxins / Q000032:analysis; D003374:Coumarins; D004988:Ethers, Cyclic / Q000032:analysis; D005456:Fluorescent Dyes; D008387:Marine Toxins / Q000032:analysis*; D009550:Nigericin / Q000032:analysis; D019319:Okadaic Acid; D035964:Oxocins; D016339:Spectrometry, Mass, Fast Atom Bombardment; D000095702:Polyether Toxins
D002922:Ciguatoxins; D003374:Coumarins; D004988:Ethers, Cyclic; D005456:Fluorescent Dyes; D008387:Marine Toxins; D009550:Nigericin; D019319:Okadaic Acid; D035964:Oxocins; C053342:brevetoxin; D000095702:Polyether Toxins