Reliability of the herpes simplex virus immunofluorescent test in corneal disease.
Herpetic ocular disease never produces well-identifiable morphological aspects. It is expensive, complicated and slow to culture Herpes simplex virus. The validity of the fluorescent monoclonal antibodies test was investigated using corneal cells with or without a sure herpetic lesion. The tests, on 36 cases without sure herpetic lesions, were always negative, while of the 42 patients with a clinically ascertained herpetic lesion, 38 were positive (90.5%) and 4 negative (9.5%), two of these on account of inadequate tissue preparations. Thus, this method can prove useful and reliable for diagnostic purposes.
Bordin
P
P
Department of Ophthalmology, State Hospital, Rovigo, Italy.
Merlin
U
U
Pugina
P
P
Benzi
G
G
Sichirollo
R
R
D000914:Antibodies, Viral / Q000032:analysis; D003315:Cornea / Q000382:microbiology; D065306:Corneal Injuries; D005129:Eye Foreign Bodies / Q000175:diagnosis; D015807:Eye Injuries, Penetrating / Q000175:diagnosis; D005455:Fluorescent Antibody Technique; D006801:Humans; D016849:Keratitis, Herpetic / Q000175:diagnosis*; D008856:Microscopy, Fluorescence; D015203:Reproducibility of Results; D018139:Simplexvirus / Q000276:immunology
D000914:Antibodies, Viral