title: Cryo-EM of the native structure of the calcium release channel/ryanodine receptor from sarcoplasmic reticulum.
abstract: The native structure of the calcium release channel (ryanodine receptor) from rabbit skeletal muscle has been analyzed in two dimensions from electron micrographs of frozen hydrated specimens. Within a resolution of 3.0 nm there is excellent agreement between the structure as seen in vitreous water and in negative stained specimens. Features seen in the three-dimensional reconstruction of the negatively stained channel can be identified in the projection of the unstained receptor.
authors.affiliation: Wadsworth Center for Laboratories and Research, New York State Department of Health, Albany 12201-0509.
authors.forename: M
authors.initials: M
authors.lastname: Radermacher
authors.forename: T
authors.initials: T
authors.lastname: Wagenknecht
authors.forename: R
authors.initials: R
authors.lastname: Grassucci
authors.forename: J
authors.initials: J
authors.lastname: Frank
authors.forename: M
authors.initials: M
authors.lastname: Inui
authors.forename: C
authors.initials: C
authors.lastname: Chadwick
authors.forename: S
authors.initials: S
authors.lastname: Fleischer
mesh_terms: D000818:Animals; D055592:Biophysical Phenomena; D001703:Biophysics; D015220:Calcium Channels / Q000648:ultrastructure*; D005615:Freezing; D008854:Microscopy, Electron; D009124:Muscle Proteins / Q000648:ultrastructure*; D011487:Protein Conformation; D011817:Rabbits; D011950:Receptors, Cholinergic / Q000648:ultrastructure*; D019837:Ryanodine Receptor Calcium Release Channel; D012519:Sarcoplasmic Reticulum / Q000648:ultrastructure
chemical_list: D015220:Calcium Channels; D009124:Muscle Proteins; D011950:Receptors, Cholinergic; D019837:Ryanodine Receptor Calcium Release Channel