Partial nucleotide sequence of the enzyme pyruvate, orthophosphate dikinase of Entamoeba histolytica HM1:IMSS.
We isolated a clone from a lambda gt11 cDNA library of E. histolytica, subcloned its insert in the vector pTZ18R and sequenced it by the method of Sanger. Sequence comparison with Genbank and of the corresponding amino acid sequence with the NBRF/PIR data bank using the FastA program, showed homologies between 54.9 and 58.5% for the nucleotides and between 48.6 and 49.4% for the amino acids with pyruvate, orthophosphate dikinases from maize, Flaveria trinervia and Bacteroides symbiosus. The sequence obtained for E. histolytica represents about 20% of the complete gene or protein sequence.
Saavedra Lira
E
E
Departamento de Microbiología, Instituto de Fisiología Celular, UNAM, México, DF.
Robinson
O
O
Pérez Montfort
R
R
D000595:Amino Acid Sequence; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D018076:DNA, Complementary / Q000235:genetics; D016054:DNA, Protozoan / Q000235:genetics*; D004748:Entamoeba histolytica / Q000201:enzymology / Q000235:genetics*; D017125:Genes, Protozoan; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010940:Plant Proteins / Q000235:genetics; D016133:Polymerase Chain Reaction; D015800:Protozoan Proteins / Q000235:genetics*; D011772:Pyruvate, Orthophosphate Dikinase / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
D001426:Bacterial Proteins; D018076:DNA, Complementary; D016054:DNA, Protozoan; D010940:Plant Proteins; D015800:Protozoan Proteins; D011772:Pyruvate, Orthophosphate Dikinase