Induction of C4-dicarboxylate transport genes by external stimuli in Rhizobium meliloti.
We have characterized two mutants of Rhizobium meliloti L5-30 obtained by random mutagenesis using Mu-lacZ that were defective in transport of C4-dicarboxylic acids. These mutants induced ineffective nodules on alfalfa. Mutations in the two strains appeared to be located in a dctA gene. Levels of dctA gene expression were determined under different environmental conditions using dctA-lacZ fusions, and beta-galactosidase activities increased in response to osmotic stress and also when the cells were incubated in medium low in calcium. The transcriptional induction of the dctA gene by environmental signals was decreased by DNA gyrase inhibitors such as novobiocin and coumermycin.
Batista
S
S
Division of Biochemistry, Instituto de Investigaciones Biológicas C., Montevideo, Uruguay.
Castro
S
S
Aguilar
O M
OM
Martínez-Drets
G
G
D049933:Aminocoumarins; D001693:Biological Transport, Active / Q000235:genetics; D003374:Coumarins / Q000494:pharmacology; D003998:Dicarboxylic Acids / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects; D016296:Mutagenesis; D009586:Nitrogen Fixation / Q000235:genetics / Q000502:physiology; D009675:Novobiocin / Q000494:pharmacology; D012231:Rhizobium / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D059005:Topoisomerase II Inhibitors
D049933:Aminocoumarins; D003374:Coumarins; D003998:Dicarboxylic Acids; D059005:Topoisomerase II Inhibitors; D009675:Novobiocin; C004628:coumermycin