Enzymatic purification of plasmid DNA.
A novel method for plasmid DNA purification using enzymes that degrade all major types of contaminating nucleic acids present in crude plasmid DNA mixtures (but not plasmid DNA) has been devised. This method is quick (can be accomplished in two hours), requires no expensive laboratory equipment (an ultracentrifuge is not necessary) and is inexpensive. Plasmid DNA purified by this methodology can be used in a variety of molecular biological techniques including restriction enzyme digestions, subcloning, sequencing, nick translation and end labeling. This plasmid purification technique will be very useful for the molecular biologist performing cloning experiments.
Isfort
R J
RJ
Miami Valley Laboratories Proctor & Gamble Co. Cincinnati, OH 45239-8707.
D004247:DNA / Q000302:isolation & purification* / Q000378:metabolism; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics; D010957:Plasmids / Q000235:genetics*; D012259:Ribonuclease, Pancreatic / Q000378:metabolism
D004247:DNA; D004262:DNA Restriction Enzymes; D012259:Ribonuclease, Pancreatic