Biphasic protein kinase C translocation in PC12 cells in response to short-term and long-term ethanol exposure.
Short-term and long-term effects of ethanol on protein kinase C (PKC) activity and PKC translocation from cytosol to membrane were examined in PC12 cells, a clonal cell line of neural crest origin. Treatment of PC12 cells with ethanol (30-100 mM) for 2 hr had no effect on PKC activity and PKC translocation. When PC12 cells were treated with 100 mM ethanol for 18, 44 and 74 hr, there was a biphasic effect on PKC translocation. At 18 and 44 hr ethanol treatment, PKC translocation was significantly (P < 0.001) increased, at 74 hr ethanol treatment, there was a significant decrease (P < 0.05). Less than 100 mM of ethanol had no effect on PKC activity and PKC translocation. Cyclic AMP and cyclic GMP-dependent protein kinase had no effect on PKC translocation. These findings indicate that biphasic PKC translocation from cytosol to membrane forms the basis of acute and chronic effects of ethanol on neurotransmission.
Virmani
M
M
Laboratory of Molecular and Cellular Neurobiology, National Institute on Alcohol and Alcoholism, Rockville, MD 20852.
Ahluwalia
B
B
D000818:Animals; D002462:Cell Membrane / Q000187:drug effects; D000242:Cyclic AMP / Q000502:physiology; D006152:Cyclic GMP / Q000502:physiology; D003600:Cytosol / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D000431:Ethanol / Q000494:pharmacology*; D016716:PC12 Cells; D011493:Protein Kinase C / Q000235:genetics* / Q000378:metabolism; D051381:Rats; D014178:Translocation, Genetic / Q000187:drug effects* / Q000235:genetics; D014407:Tumor Cells, Cultured / Q000187:drug effects*
D000431:Ethanol; D000242:Cyclic AMP; D011493:Protein Kinase C; D006152:Cyclic GMP