title: [Gallbladder cancer: immunohistochemical expression of CA-19-9, epithelial membrane antigen, dupan-2 and carcinoembryonic antigen].
abstract: The immunohistochemical expression of CA19-9, epithelial membrane antigen (EMA), and carcinoembryonic antigen (CEA) was studied in 103 tissue samples of gallbladder cancer and 25 samples of non tumoral gallbladder lesions. CA19-9 and EMA were positive in over 90% of cancers and non tumoral lesions. Dupan-2 expression was observed in 100% of non tumoral lesions and 78% of cancers. CEA expression was observed in 12% of non tumoral lesions and 89% of cancers. The magnitude of immunohistochemical staining was moderate or intense for all antibodies, except Dupan-2. No differences were observed in the location of positive staining in superficial or deep parts of the tumor. In these lesions the positive staining was cytoplasmatic with a granular and irregular pattern; on the contrary, in the non tumoral lesions, staining was seen in the apical parts of the cell. Calculated sensitivity, specificity and positive predictive value for CEA was 89%, 88% and 96% respectively. If future studies disclose a good correlation between serological and immunohistochemical detection of this antigen, its determination would be potentially useful in clinical grounds.
authors.affiliation: Unidad de Anatomía Patológica y Citopatología, Facultad de Medicina, Universidad de la Frontera, Hospital Regional de Temuco, Chile.
authors.forename: I
authors.initials: I
authors.lastname: Roa
authors.forename: J C
authors.initials: JC
authors.lastname: Araya
authors.forename: T
authors.initials: T
authors.lastname: Shiraisch
authors.forename: R
authors.initials: R
authors.lastname: Yatani
authors.forename: I
authors.initials: I
authors.lastname: Wistuba
authors.forename: M
authors.initials: M
authors.lastname: Villaseca
authors.forename: X
authors.initials: X
authors.lastname: de Aretxabala
mesh_terms: D000951:Antigens, Neoplasm / Q000032:analysis*; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000032:analysis; D014408:Biomarkers, Tumor / Q000032:analysis*; D002272:Carcinoembryonic Antigen / Q000032:analysis; D005260:Female; D005706:Gallbladder Neoplasms / Q000276:immunology* / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008562:Membrane Glycoproteins / Q000032:analysis; D018396:Mucin-1
chemical_list: D000951:Antigens, Neoplasm; D015295:Antigens, Tumor-Associated, Carbohydrate; D014408:Biomarkers, Tumor; D002272:Carcinoembryonic Antigen; C042317:DU-PAN-2 antigen, human; D008562:Membrane Glycoproteins; D018396:Mucin-1
vernacular_title: Cancer de la vesicula biliar: expression inmunohistoquimica del CA19-9, antigeno de membrana epitelial, Dupan-2 Y antigeno carcinoembrionario.