title: Protein phosphorylation regulated by cyclic nucleotide-dependent protein kinases in cell extracts and in intact human lymphocytes.
abstract: A specific 46,000/50,000 molecular weight protein substrate for both cAMP-dependent protein kinase (cAK) and cGMP-dependent protein kinase (cGK) extensively characterized and purified from human platelets was found to be present also in human T-lymphocytes, B-lymphocytes and other cells and tumour cell lines. This protein termed vasodilator-stimulated phosphoprotein (VASP) was present in cytosol and membranes of lymphocytes. Addition of exogenous purified cAK or cGK to lymphocyte cytosol or membranes converted 80-90% of VASP to its phosphoform. Endogenous VASP phosphorylation in both cytosol and membranes was stimulated by the addition of cAMP but not by cGMP. With intact lymphocytes, prostaglandin E1 (PGE1) and prostaglandin E2 (PGE2) induced an increase of cAMP and converted 70% of VASP to its phosphoform. In contrast, an increase of cGMP was not associated with VASP phosphorylation although cGK was detected in lymphocytes. These data support the hypothesis that VASP phosphorylation may be an important component of cAMP-mediated regulation of lymphocyte function.
authors.affiliation: Labor für klinische Biochemie and Kinderklinik der Universität, Würzburg, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Halbrügge
authors.forename: M
authors.initials: M
authors.lastname: Eigenthaler
authors.forename: C
authors.initials: C
authors.lastname: Polke
authors.forename: U
authors.initials: U
authors.lastname: Walter
mesh_terms: D002457:Cell Extracts; D002460:Cell Line; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000097:blood / Q000502:physiology; D006152:Cyclic GMP / Q000097:blood / Q000502:physiology; D006801:Humans; D008214:Lymphocytes / Q000201:enzymology / Q000378:metabolism*; D009000:Monocytes / Q000201:enzymology / Q000378:metabolism; D010750:Phosphoproteins / Q000097:blood / Q000502:physiology*; D010766:Phosphorylation / Q000187:drug effects; D011494:Protein Kinases / Q000097:blood / Q000502:physiology*; D014665:Vasodilator Agents / Q000097:blood / Q000494:pharmacology
chemical_list: D002457:Cell Extracts; D010750:Phosphoproteins; D014665:Vasodilator Agents; D000242:Cyclic AMP; D011494:Protein Kinases; D006152:Cyclic GMP