Morphogenesis and differentiation of Dictyostelium cells interacting with immobilized glucosides: dependence on DIF production.
Previous work has shown that multicellular morphogenesis of submerged Dictyostelium cells is inhibited when they bind to glucosides covalently linked to polyacrylamide gels. The amoebae aggregate normally, but then the aggregates repeatedly disperse and reaggregate, whereas control cells go on to form tight aggregates. We have investigated the role of the stalk cell differentiation inducing factors (DIFs) in this process. In the presence of cyclic AMP, amoebae submerged at high cell density accumulate DIF and differentiate into stalk cells. We find that stalk cell differentiation is inhibited by interaction of the cells with glucoside gels in these conditions, but can be restored by the addition of exogenous DIF-1. Since the responsiveness of cells to DIF-1 is not altered, it appears likely that the effect of the glucoside gel is to block DIF-1 production. Further, the addition of DIF-1 or DIF-2 stimulates the formation of tight aggregates by cells developing on glucoside gels in the absence of cyclic AMP, thus preventing the rounds of aggregation and disaggregation otherwise seen. This suggests a role for DIF in morphogenesis as well as in controlling cell differentiation. We propose a model in which immobilized glucosides activate a specific receptor ("food sensor") which drives the amoebae toward the vegetative state and inhibits DIF accumulation. DIF, on the other hand, induces tight aggregate formation and so locks the amoebae into the developmental program.
Gambino
M
M
Department of Clinical and Biological Sciences, University of Turin (Torino), Italy.
Kay
R R
RR
Bozzaro
S
S
D000818:Animals; D002449:Cell Aggregation / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D000242:Cyclic AMP / Q000494:pharmacology; D004023:Dictyostelium / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D005782:Gels; D005960:Glucosides / Q000378:metabolism / Q000494:pharmacology*; D006588:Hexanones / Q000378:metabolism* / Q000494:pharmacology; D007700:Kinetics; D009024:Morphogenesis / Q000187:drug effects; D009621:Nogalamycin / Q000494:pharmacology; D013170:Spores / Q000187:drug effects / Q000502:physiology; D013997:Time Factors
D005782:Gels; D005960:Glucosides; D006588:Hexanones; C053433:1-((3,5-dichloro)-2,6-dihydroxy-4-methoxyphenyl)-1-hexanone; D000242:Cyclic AMP; D009621:Nogalamycin