title: The soluble form of two N-terminal domains of the poliovirus receptor is sufficient for blocking viral infection.
abstract: By means of deleting a C-terminal portion of the open reading frame of the poliovirus receptor cDNA, and by vaccinia virus-mediated overexpression we have produced a protein corresponding to the first two N-terminal Ig-like domains of the poliovirus receptor. This protein that lacked the third Ig-like domain, the transmembrane region and most of the intracellular C-terminal tail was detected in the medium of vaccinia virus infected cells. The properties of the truncated PVR cDNA were further characterized by in vitro translation and modification. The molecular weight of the unmodified protein was found to be 27 kDa; translation in the presence of dog pancreas microsomes led to an increase in molecular weights which we attribute to N-glycosylation. Upon incubation with poliovirus at 37 degrees C, the vaccinia-virus generated protein specifically reduced infectivity of poliovirus. Sucrose gradients of poliovirus particles derived after incubation with the protein showed the induction of a slower sedimenting particle (135S). Our experiments suggest that the two N-terminal domains of the poliovirus receptor in soluble form are sufficient for the conversion of poliovirus into a non-infectious particle.
authors.affiliation: Department of Microbiology, State University of New York, Stony Brook 11794.
authors.forename: A
authors.initials: A
authors.lastname: Zibert
authors.forename: H C
authors.initials: HC
authors.lastname: Selinka
authors.forename: O
authors.initials: O
authors.lastname: Elroy-Stein
authors.forename: E
authors.initials: E
authors.lastname: Wimmer
mesh_terms: D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D004279:DNA, Viral / Q000235:genetics; D004285:Dogs; D006801:Humans; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D017955:Poliovirus / Q000235:genetics* / Q000472:pathogenicity / Q000502:physiology; D011991:Receptors, Virus / Q000235:genetics* / Q000502:physiology; D011995:Recombination, Genetic; D012995:Solubility; D014616:Vaccinia virus / Q000235:genetics
chemical_list: D004279:DNA, Viral; D011991:Receptors, Virus