title: Contact allergens modulate the expression of MHC class II molecules on murine epidermal Langerhans cells by endocytotic mechanisms.
abstract: MHC class II molecules play an important role during the sensitization phase of allergic contact dermatitis. To study the influence of contact allergens on the expression of these molecules by murine epidermal Langerhans cells (LC), we performed a flow-cytofluorometric analysis of the Ia-antigen expression after in vivo application of contact allergens. A distinct decrease in the Ia-antigen expression of the entire LC population was noticed 3 h after in vivo application of the contact allergen 2,4-dinitrofluorobenzene (DNFB). This decrease was transient and balanced 24 h after in vivo application of DNFB. A downregulation was also detectable after in vivo application of the contact allergens 1-chloro-2,4-dinitrobenzene (DNCB), oxazolone, K2Cr2O7, 2,4,6-trinitrochlorobenzene (TNCB), and toxic concentrations of the irritant compound sodium dodecyl sulfate (SDS). In vitro studies showed that freshly prepared as well as 3-d cultured LC downregulated their Ia-antigen expression in the presence of DNFB, which was used as a model compound. This decrease was not inhibited by the MHC class II molecule transport-inhibitor brefeldin A nor by the ionophore monensin. The inhibition of receptor-mediated endocytosis with hypertonic media (0.45 M sucrose) abolished the DNFB-mediated downregulation of Ia-antigen expression. An accelerated clearance of cell-surface-expressed antibody-labeled IA molecules was detectable in the presence of DNFB. Internalization studies carried out with peroxidase-labeled anti-IA-antibody complexes showed remarkable alterations in the intracellular distribution of endocytosed material under the influence of subtoxic concentrations of DNFB, DNCB, K2Cr2O7, and TNCB. The irritant substance sodium dodecyl sulfate (SDS) influenced the intracellular distribution pattern of internalized material only when used in toxic concentrations. An augmented participation of MHC class II molecules in endocytotic processes is mediated by reactive substances like contact allergens and might contribute to the processing and presentation of these compounds.
authors.affiliation: Department of Dermatology, University of Mainz, Germany.
authors.forename: D
authors.initials: D
authors.lastname: Becker
authors.forename: U
authors.initials: U
authors.lastname: Neiss
authors.forename: S
authors.initials: S
authors.lastname: Neis
authors.forename: K
authors.initials: K
authors.lastname: Reske
authors.forename: J
authors.initials: J
authors.lastname: Knop
mesh_terms: D000485:Allergens / Q000502:physiology*; D000818:Animals; D000911:Antibodies, Monoclonal; D020126:Brefeldin A; D003517:Cyclopentanes / Q000494:pharmacology; D003877:Dermatitis, Contact / Q000276:immunology*; D004705:Endocytosis / Q000502:physiology; D005260:Female; D000949:Histocompatibility Antigens Class II / Q000187:drug effects / Q000276:immunology* / Q000502:physiology; D006982:Hypertonic Solutions / Q000494:pharmacology; D007801:Langerhans Cells / Q000276:immunology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008985:Monensin / Q000494:pharmacology
chemical_list: D000485:Allergens; D000911:Antibodies, Monoclonal; D003517:Cyclopentanes; D000949:Histocompatibility Antigens Class II; D006982:Hypertonic Solutions; D020126:Brefeldin A; D008985:Monensin