Prostaglandin E2 binding site distribution and subtype classification in the rabbit iris-ciliary body.
The distribution and characteristics of specific binding sites for tritium labeled prostaglandin E2 (3H-PGE2) were examined in membrane preparations from rabbit iris-sphincter, iris and ciliary body. The majority of 3H-PGE2 specific binding sites were found in the ciliary body (46%) followed by the iris (37%) and the iris-sphincter muscle (5%). Scatchard analysis of saturable 3H-PGE2 binding sites in the ciliary body indicated a single binding site with a Kd of 2.81 nM and Bmax value of 84 fmoles bound/mg protein. Competition by agonists selective for the EP1, EP2 and EP3 receptor subtypes of the EP (PGE2) prostanoid receptor indicated that the majority of rabbit ciliary body 3H-PGE2 binding sites are of the EP2 subtype. Incomplete displacement of labeled 3H-PGE2 from its binding sites by the EP2 selective agonist 11-deoxy PGE1 suggests the presence of additional EP or non-EP binding sites. There was essentially no binding to EP1 receptor sites as defined by the displacement of 3H-PGE2 by 17-phenyl-trinor PGE2. A weak displacement of 3H-PGE2 by the EP3/EP1 specific agonist, sulprostone, may account for the presence of a small number of EP3 specific binding sites in this tissue. The predominant distribution of PGE2 binding sites in the ciliary body and their identification as EP2 selective, supports recent functional studies where topical application of prostanoids with EP2 but not EP1 or EP3 agonist activity resulted in breakdown of the blood-aqueous barrier.
Csukas
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S
Department of Ophthalmology and Visual Sciences, Kentucky Lions Eye Research Institute, University of Louisville, School of Medicine 40292.
Bhattacherjee
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P
Rhodes
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L
Paterson
C A
CA
D000818:Animals; D001667:Binding, Competitive / Q000502:physiology; D002924:Ciliary Body / Q000378:metabolism*; D007498:Iris / Q000378:metabolism*; D008566:Membranes / Q000378:metabolism; D009130:Muscle, Smooth / Q000378:metabolism*; D011458:Prostaglandins E; D011817:Rabbits; D011869:Radioligand Assay; D011982:Receptors, Prostaglandin / Q000145:classification / Q000378:metabolism*; D018078:Receptors, Prostaglandin E
D011458:Prostaglandins E; D011982:Receptors, Prostaglandin; D018078:Receptors, Prostaglandin E