Bovine leukemia virus gene expression in vivo.
The in vivo transcriptional status of bovine leukemia virus was assessed at three stages of infection during the progression of the disease: aleukemic stage, persistent lymphocytosis, and leukemia/lymphosarcoma. Bovine leukemia virus transcripts could be amplified from total or cytoplasmic enriched lymphocyte RNA by reverse transcription polymerase chain reaction in cells from all but a few aleukemic animals. With primer pairs diagnostic for differentially spliced transcripts (full length-genomic, envelope, tax/rex, and alternatively spliced), a trend toward exclusion of both full-length and envelope RNAs, with retention of the tax/rex message, appears as leukemia/lymphosarcoma develops.
Haas
L
L
Department of Microbiology, Parasitology, and Immunology, New York State College of Veterinary Medicine, Cornell University, Ithaca 14853.
Divers
T
T
Casey
J W
JW
D001483:Base Sequence; D015139:Blotting, Southern; D015870:Gene Expression; D005814:Genes, Viral; D016355:Genes, pX; D001909:Leukemia Virus, Bovine / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000378:metabolism; D014158:Transcription, Genetic
D012367:RNA, Viral