title: Evidence for an internal pool of nucleoside transporters in mammalian reticulocytes.
abstract: Comparisons of the site specific binding of nitrobenzylthioinosine (NBMPR) to intact and lysed red cells from various mammalian and avian species suggest the presence of a cytoplasmic pool of nucleoside transporters. In some species the cytoplasmic pool is about 50% of the total (mouse). On the average, the cytoplasmic pool is approx. 20% of the surface pool of NBMPR-binding sites. In sheep reticulocytes, both pools disappear in an energy-dependent manner during the maturation of the reticulocyte in vitro.
authors.affiliation: Department of Biochemistry, McGill University, Montreal, Canada.
authors.forename: L
authors.initials: L
authors.lastname: Liang
authors.forename: R M
authors.initials: RM
authors.lastname: Johnstone
mesh_terms: D002731:4-Chloromercuribenzenesulfonate / Q000494:pharmacology; D000345:Affinity Labels; D000818:Animals; D001665:Binding Sites; D001798:Blood Proteins / Q000378:metabolism; D002352:Carrier Proteins / Q000378:metabolism*; D002478:Cells, Cultured; D002642:Chick Embryo; D004910:Erythrocyte Membrane / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism*; D009245:NADH Dehydrogenase / Q000378:metabolism; D033703:Nucleoside Transport Proteins; D012156:Reticulocytes / Q000378:metabolism*; D012756:Sheep; D013868:Thioinosine / Q000031:analogs & derivatives / Q000378:metabolism; D014357:Trypsin / Q000378:metabolism
chemical_list: D000345:Affinity Labels; D001798:Blood Proteins; D002352:Carrier Proteins; D008565:Membrane Proteins; D033703:Nucleoside Transport Proteins; D013868:Thioinosine; D002731:4-Chloromercuribenzenesulfonate; D009245:NADH Dehydrogenase; D014357:Trypsin; C001789:4-nitrobenzylthioinosine