title: Anti-proliferative and cytotoxic activity of surface-associated material from periodontopathogenic bacteria.
abstract: The easily solubilized surface-associated material from three bacterial species associated with periodontal diseases, Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis and Eikenella corrodens, produced dose-dependent inhibition of thymidine incorporation by human fibroblasts, the human monocytic cell line U937 and guinea pig epidermal cells. In contrast, lipopolysaccharides from A. actinomycetemcomitans and P. gingivalis were either inactive or substantially less active over the dose range tested. One of the constituents of surface-associated material from a 'non-leucotoxic' strain of A. actinomycetemcomitans was highly cytotoxic to human peripheral blood polymorphonuclear cells, with 50% killing from less than 1 ng/ml. A constituent of the surface-associated material from P. gingivalis was approximately one log order less active. The lipopolysaccharides from these bacteria were at least three log orders less active in neutrophil killing. These findings add weight to the hypothesis that easily solubilized exopolymers from periodontopathogens play a major part in the pathology of periodontal diseases.
authors.affiliation: MaxilloFacial Surgery and Oral Medicine Research Unit, University of London, U.K.
authors.forename: S
authors.initials: S
authors.lastname: Meghji
authors.forename: M
authors.initials: M
authors.lastname: Wilson
authors.forename: B
authors.initials: B
authors.lastname: Henderson
authors.forename: D
authors.initials: D
authors.lastname: Kinane
mesh_terms: D016976:Aggregatibacter actinomycetemcomitans / Q000472:pathogenicity; D000818:Animals; D001425:Bacterial Outer Membrane Proteins; D002455:Cell Division / Q000187:drug effects*; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis*; D016124:Eikenella corrodens / Q000472:pathogenicity; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D006168:Guinea Pigs; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects / Q000378:metabolism; D008070:Lipopolysaccharides; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism; D010510:Periodontal Diseases / Q000382:microbiology*; D016966:Porphyromonas gingivalis / Q000472:pathogenicity; D013936:Thymidine / Q000378:metabolism
chemical_list: D001425:Bacterial Outer Membrane Proteins; D008070:Lipopolysaccharides; D004247:DNA; D013936:Thymidine