Atypical rotavirus genomic patterns identified by polyacrylamide gel electrophoresis.
Two atypical "short" rotavirus genomic profiles were detected in stool specimens following the testing of 84 children with diarrhoea. Both rotaviruses were identified as Group A, and confirmation was performed by blocking assay using antiserum against simian rotavirus SA-11. This report underscores the importance of screening on the molecular level to detect atypical or unusual rotaviruses that would normally proceed unrecognised by testing with any of the commercial antigen detection kits.
Lipson
S M
SM
Jane and Dayton Brown and Dayton T. Brown, Jr, Virology Laboratory, Department of Medicine, North Shore University Hospital--Cornell University Medical College, Manhasset, New York.
Kaplan
M H
MH
D002675:Child, Preschool; D003968:Diarrhea, Infantile / Q000382:microbiology*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D012330:RNA, Double-Stranded / Q000032:analysis*; D012367:RNA, Viral / Q000032:analysis*; D012401:Rotavirus / Q000145:classification / Q000235:genetics*; D012400:Rotavirus Infections / Q000382:microbiology*
D012330:RNA, Double-Stranded; D012367:RNA, Viral