Direct visualization of serotonin1D receptors in the human brain using a new iodinated radioligand.
The development of the new ligand serotonin-5-O-carboxymethyl-glycyl [125I]tyrosinamide (abbreviated [125I]GTI) allows for the direct visualization of serotonin1B and serotonin1D (5-HT1B/1D) sites. Autoradiographic techniques were used to demonstrate the selective binding of this ligand to 5-HT1D sites in human post-mortem brain materials. The distribution of [125I]GTI binding sites was compared to [3H]5-HT sites in the presence of different displacers. The results show the selective binding of [125I]GTI to sites in the basal ganglia and substantia nigra which corresponds to 5-HT1D receptors.
Palacios
J M
JM
Preclinical Research Sandoz Pharma Ltd., Basel, Switzerland.
Waeber
C
C
Bruinvels
A T
AT
Hoyer
D
D
D000368:Aged; D001345:Autoradiography / Q000379:methods; D001479:Basal Ganglia / Q000378:metabolism; D001667:Binding, Competitive; D001921:Brain / Q000378:metabolism*; D004151:Dipeptides / Q000378:metabolism*; D006165:Guanylyl Imidodiphosphate / Q000494:pharmacology; D006801:Humans; D007457:Iodine Radioisotopes; D007700:Kinetics; D008636:Mesencephalon / Q000378:metabolism; D011869:Radioligand Assay; D011985:Receptors, Serotonin / Q000032:analysis / Q000378:metabolism*; D012701:Serotonin / Q000031:analogs & derivatives* / Q000378:metabolism*
D004151:Dipeptides; D007457:Iodine Radioisotopes; D011985:Receptors, Serotonin; C072128:serotonin-O-carboxymethyl-Gly-Tyr; D012701:Serotonin; D006165:Guanylyl Imidodiphosphate