Human genomic sequences corresponding to murine CD3 eta-related transcripts: lack of conservation or expression of homologous human products.
We have cloned and sequenced human genomic DNA homologous to exons 9 and 10 of the CD3 zeta/eta/theta locus. Although there are open reading frames within the human sequences corresponding to the translated portions of murine exons 9 and 10, we find no evidence of conservation of the encoded polypeptide product. Furthermore, using oligonucleotides derived from these homologous sequences, we are unable to detect human CD3 eta- or CD3 theta-like transcripts by polymerase chain reaction amplification of reverse-transcribed RNA from a variety of human lymphoid tissues. Despite the absence of evidence for conservation of human CD3 eta and CD3 theta, there is a surprising degree of similarity between human and murine nucleotide sequences, not only for exons 9 and 10 (78% and 70%, respectively), but also for the 9/10 intron (71%). A possible mechanism for this conservation is discussed.
Lerner
A
A
Laboratory of Immunobiology, Harvard Medical School, Boston, MA.
Diener
A C
AC
Reinherz
E L
EL
Clayton
L K
LK
D000818:Animals; D015703:Antigens, CD / Q000235:genetics; D000945:Antigens, Differentiation, T-Lymphocyte / Q000235:genetics*; D000951:Antigens, Neoplasm / Q000235:genetics; D001483:Base Sequence; D017252:CD3 Complex; D005091:Exons; D015894:Genome, Human; D006801:Humans; D017730:Ki-1 Antigen; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011948:Receptors, Antigen, T-Cell / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic
D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D000951:Antigens, Neoplasm; D017252:CD3 Complex; D017730:Ki-1 Antigen; D011948:Receptors, Antigen, T-Cell