Presence of luteinizing hormone-releasing hormone binding sites in cultured porcine lymphocytes.
Membrane-enriched homogenates of fresh and cultured (48 h) porcine lymphocytes were assayed for the presence of specific LHRH receptors by saturation and displacement analysis using [D-Ser-(TBU)6, des-Gly-NH2(10)] LHRH-EA as the labeled and unlabeled ligand. Membrane-enriched homogenates of porcine pituitaries served as positive controls while porcine granulosa cell membranes and crude liver homogenates served as negative controls. Specific high-affinity LHRH receptors were found in porcine pituitaries (Kd = 0.3 nM) and cultured lymphocytes (Kd = 13 nM) but not in fresh lymphocytes. No specific binding was observed in negative control tissues. Porcine lymphocytes have measurable high-affinity LHRH receptors after 48 h of culture.
Standaert
F E
FE
Department of Animal Sciences, Washington State University, Pullman 99164-6320.
Chew
B P
BP
De Avila
D
D
Reeves
J J
JJ
D000818:Animals; D001667:Binding, Competitive; D002064:Buserelin / Q000378:metabolism; D002478:Cells, Cultured; D005260:Female; D006107:Granulosa Cells / Q000378:metabolism; D008214:Lymphocytes / Q000378:metabolism*; D010903:Pituitary Gland, Anterior / Q000378:metabolism; D011966:Receptors, LHRH / Q000378:metabolism*; D013552:Swine / Q000097:blood*
D011966:Receptors, LHRH; D002064:Buserelin