Effects of androgen treatment on adenylate cyclase system in rat hepatic membranes.
Effects of androgen treatment of young female rats on glucagon- and catecholamine-sensitive adenylate cyclase activity and adrenergic receptors of hepatic membranes have been studied. Injections of testosterone propionate for 7 days showed a significant decrease in the adenylate cyclase activity responding to isoproterenol and glucagon. The decrease in hormonal stimulation of the enzyme was accompanied with the fall in activation by non-hormonal stimuli, such as forskolin, sodium fluoride, Gpp(NH)p and Mn, without any changes in the number and the affinity of beta-adrenergic receptors of the membrane. These results suggest that androgens exert post-receptor effects by inhibiting the activity of the catalytic unit of adenylate cyclase system in rat hepatic membranes.
Shima
S
S
Department of Biochemistry, St. Marianna University, School of Medicine, Kanagawa, Japan.
D000067956:Adenylyl Cyclase Inhibitors; D000262:Adenylyl Cyclases / Q000187:drug effects* / Q000378:metabolism; D000728:Androgens / Q000494:pharmacology*; D000818:Animals; D001667:Binding, Competitive; D001786:Blood Glucose / Q000187:drug effects; D002395:Catecholamines / Q000494:pharmacology; D002478:Cells, Cultured; D003921:Diabetes Mellitus, Experimental / Q000201:enzymology; D005260:Female; D005934:Glucagon / Q000494:pharmacology; D007545:Isoproterenol / Q000494:pharmacology; D007700:Kinetics; D008099:Liver / Q000187:drug effects / Q000201:enzymology* / Q000648:ultrastructure; D008566:Membranes / Q000187:drug effects / Q000201:enzymology; D051381:Rats; D011919:Rats, Inbred Strains; D011943:Receptors, Adrenergic, beta / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D013268:Stimulation, Chemical; D013739:Testosterone / Q000494:pharmacology
D000067956:Adenylyl Cyclase Inhibitors; D000728:Androgens; D001786:Blood Glucose; D002395:Catecholamines; D011943:Receptors, Adrenergic, beta; D013739:Testosterone; D005934:Glucagon; D000262:Adenylyl Cyclases; D007545:Isoproterenol