Influence of arachidonic acid metabolites and steroids on function of bovine polymorphonuclear neutrophils.
Polymorphonuclear neutrophils (PMN) from 4 ovariectomized healthy cows were incubated with 0 (control), 10(-8), 10(-7), and 10(-6) M arachidonic acid metabolites of the cyclo- and lipoxygenase pathways for 30 minutes, and with steroids for 2 hours. Immediately after incubation, PMN were subjected to the following function assays: chemotaxis against zymosan-activated serum, chemotaxis against arachidonic acid metabolite or steroid at the doses given (only control PMN were tested), random migration, ingestion of 125I-iododeoxyuridine-labeled Staphylococcus aureus (125I-IdUR-S aureus), iodination of proteins, cytochrome C reduction, antibody-independent and -dependent cell-mediated cytotoxicity (AICC and ADCC). Prostaglandin F2 alpha was chemoattractant and stimulated ingestion of 125I-IdUR-S aureus. Prostaglandin E2 stimulated cytochrome C reduction, whereas prostacyclin inhibited iodination of proteins. Thromboxane B2 stimulated ADCC. Leukotriene B4 was chemoattractant for bovine PMN and stimulated random migration and AICC. 5-Hydroxyeicosatetraenoic acid was also chemoattractant, but inhibited ingestion of 125I-IdUR-S aureus. 15-Hydroxyeicosatetraenoic acid was chemoattractant and decreased ADCC. Lipoxin A4 stimulated random migration, whereas lipoxin B4 inhibited chemotaxis against zymosan-activated serum, but was chemoattractant and stimulated cytochrome C reduction. 12-Hydroxyhepadecatrienoic acid and 12-hydroxyeicosatetraenoic acid did not influence any of the PMN functions tested. Of the steroids tested, cortisol increased ADCC, and progesterone stimulated cytochrome C reduction, but decreased ADCC. 17 beta-Estradiol and estrone were chemoattractant and stimulated cytochrome C reduction. In addition, estrone also stimulated random migration.(ABSTRACT TRUNCATED AT 250 WORDS)
Hoedemaker
M
M
Department of Veterinary Biosciences, College of Veterinary Medicine, University of Illinois, Urbana 61801.
Lund
L A
LA
Wagner
W C
WC
D000818:Animals; D000920:Antibody-Dependent Cell Cytotoxicity / Q000187:drug effects; D001095:Arachidonic Acids / Q000494:pharmacology*; D002417:Cattle / Q000502:physiology*; D002478:Cells, Cultured; D002634:Chemotaxis, Leukocyte; D015237:Dinoprost / Q000494:pharmacology; D015232:Dinoprostone / Q000494:pharmacology; D011464:Epoprostenol / Q000494:pharmacology; D004958:Estradiol / Q000494:pharmacology; D004970:Estrone / Q000494:pharmacology; D006893:Hydroxyeicosatetraenoic Acids / Q000494:pharmacology; D007975:Leukotriene B4 / Q000494:pharmacology; D044045:Lipoxins; D009504:Neutrophils / Q000187:drug effects* / Q000276:immunology / Q000502:physiology; D011374:Progesterone / Q000494:pharmacology; D013211:Staphylococcus aureus / Q000276:immunology; D013256:Steroids / Q000494:pharmacology*; D013929:Thromboxane B2 / Q000494:pharmacology
D001095:Arachidonic Acids; D006893:Hydroxyeicosatetraenoic Acids; D044045:Lipoxins; D013256:Steroids; D007975:Leukotriene B4; D004970:Estrone; D011374:Progesterone; D004958:Estradiol; D013929:Thromboxane B2; C042651:lipoxin B4; D015237:Dinoprost; D011464:Epoprostenol; D015232:Dinoprostone