title: Induction of differentiation of the human promyelocytic cell line HL-60 by activin/EDF.
abstract: A human promyelocytic cell line, HL-60, treated with activin/EDF was found to differentiate into monocyte/macrophage-like cells. This was shown not only by morphology but by the loss of myeloperoxidase granules and the appearance of nonspecific esterase. Dose-dependent inhibition of the differentiation by follistatin, an activin-binding protein, confirmed that it was indeed caused by activin. Thus, activin/EDF exerts its effect on hematopoietic cells not only on erythroid differentiation but also on at least a part of myeloid cell differentiation.
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Saitama Medical School, Japan.
authors.forename: R
authors.initials: R
authors.lastname: Yamada
authors.forename: T
authors.initials: T
authors.lastname: Suzuki
authors.forename: M
authors.initials: M
authors.lastname: Hashimoto
authors.forename: Y
authors.initials: Y
authors.lastname: Eto
authors.forename: K
authors.initials: K
authors.lastname: Shiokawa
authors.forename: M
authors.initials: M
authors.lastname: Muramatsu
mesh_terms: D028341:Activins; D001665:Binding Sites; D002454:Cell Differentiation / Q000187:drug effects; D003594:Cytoplasmic Granules / Q000648:ultrastructure; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D038681:Follistatin; D006023:Glycoproteins / Q000494:pharmacology; D006651:Histocytochemistry; D006801:Humans; D007265:Inhibins / Q000378:metabolism / Q000494:pharmacology*; D015473:Leukemia, Promyelocytic, Acute / Q000378:metabolism / Q000473:pathology*; D009195:Peroxidase / Q000378:metabolism; D014407:Tumor Cells, Cultured
chemical_list: D038681:Follistatin; D006023:Glycoproteins; D028341:Activins; D007265:Inhibins; D004815:Epidermal Growth Factor; D009195:Peroxidase