title: A rapid method for starting a culture for the establishment of Epstein-Barr virus-transformed human lymphoblastoid cell lines.
abstract: We developed a simple and efficient procedure for the establishment of Epstein-Barr virus-transformed human lymphoblastoid cell lines. B-lymphocytes were obtained by centrifugation after hemolysis of red cells with a hemolysis buffer, instead of Ficoll-Parque gradient. We can start a primary culture within 15 min by using this method.
authors.affiliation: Division of Medical Genetics, Saitama Children's Medical Center, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Fukushima
authors.forename: H
authors.initials: H
authors.lastname: Ohashi
authors.forename: K
authors.initials: K
authors.lastname: Wakui
authors.forename: T
authors.initials: T
authors.lastname: Nishida
authors.forename: T
authors.initials: T
authors.lastname: Oh-ishi
mesh_terms: D001402:B-Lymphocytes / Q000166:cytology*; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral; D004854:Herpesvirus 4, Human / Q000502:physiology; D006801:Humans; D013997:Time Factors