title: Inhibition of cell cycle progression by antisense oligodeoxynucleotides.
abstract: We have used the antisense strategy to study the role of certain genes in cell cycle progression. In particular, we used antisense oligodeoxynucleotides to study: (1) the role of the IGF-1 receptor in the control of cell proliferation; and (2) the sequence of gene expression during the cell cycle. Our results can be summarized as follows: (1) the activation of the IGF-1 receptor by its ligand, IGF-1, is an obligatory step in the proliferation of fibroblasts and hemopoietic cells; and (2) the expression of DNA synthesis genes, such as PCNA, DNA polymerase alpha, and cdc2, is dependent on the expression of previous genes. A tentative temporal order is: c-myc > c-myb > IGF-1 receptor > DNA synthesis genes.
authors.affiliation: Jefferson Cancer Institute, Jefferson Medical College, Philadelphia, Pennsylvania 19107-5541.
authors.forename: R
authors.initials: R
authors.lastname: Baserga
authors.forename: K
authors.initials: K
authors.lastname: Reiss
authors.forename: H
authors.initials: H
authors.lastname: Alder
authors.forename: Z
authors.initials: Z
authors.lastname: Pietrzkowski
authors.forename: E
authors.initials: E
authors.lastname: Surmacz
mesh_terms: D000818:Animals; D002453:Cell Cycle / Q000187:drug effects*; D002478:Cells, Cultured; D016259:Genes, myc / Q000235:genetics; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000187:drug effects; D008957:Models, Genetic; D009838:Oligodeoxyribonucleotides / Q000494:pharmacology*; D016376:Oligonucleotides, Antisense / Q000494:pharmacology*; D010835:Phytohemagglutinins / Q000494:pharmacology; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D017526:Receptor, IGF Type 1 / Q000235:genetics*
chemical_list: D009838:Oligodeoxyribonucleotides; D016376:Oligonucleotides, Antisense; D010835:Phytohemagglutinins; D012333:RNA, Messenger; D017526:Receptor, IGF Type 1