Astrocytic localization of the messenger RNA encoding the isoenzyme B of inositol (1,4,5) 3-kinase in the human brain.
The calcium-mobilizing second messenger inositol 1,4,5-trisphosphate (InsP3) is phosphorylated to inositol 1,3,4,5-tetrakisphosphate, another putative second messenger, through the activity of the enzyme InsP3 3-kinase. The cDNAs encoding two such isozymes have been recently isolated from a human hippocampal cDNAs library. We have previously reported the neuronal localization of the A form (Mailleux et al., Neurosci. Lett., 137 (1992) 69-71) and we here demonstrate the presence of the messenger RNA for the B form in human astrocytes.
Mailleux
P
P
Laboratory of Neurophysiology, Faculty of Medicine, Université Libre de Bruxelles, Belgium.
Takazawa
K
K
Albala
N
N
Erneux
C
C
Vanderhaeghen
J J
JJ
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001253:Astrocytes / Q000201:enzymology*; D001921:Brain / Q000201:enzymology*; D002525:Cerebellar Cortex / Q000201:enzymology; D015342:DNA Probes; D005260:Female; D006801:Humans; D007527:Isoenzymes / Q000235:genetics*; D008297:Male; D015345:Oligonucleotide Probes; D010770:Phosphotransferases / Q000235:genetics*; D017853:Phosphotransferases (Alcohol Group Acceptor); D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism*; D013462:Sulfur Radioisotopes
D015342:DNA Probes; D007527:Isoenzymes; D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D013462:Sulfur Radioisotopes; D010770:Phosphotransferases; D017853:Phosphotransferases (Alcohol Group Acceptor); C051038:Inositol 1,4,5-trisphosphate 3-kinase