Assignment of the human CRABP-II gene to chromosome 1q21 by nonisotopic in situ hybridization.
Two highly conserved forms of cellular retinoic acid binding protein (CRABP-I and CRABP-II) have been described, and one, CRABP-II, is highly expressed in human skin. We have utilized a 10-kb fragment containing the human CRABP-II (hCRABP-II) gene (isolated from a human genomic library) to localize hCRABP-II to human chromosome 1 band q21 by fluorescence in situ hybridization. Localization to 1q was confirmed by hybridization of a hCRABP-II cDNA clone against a human-mouse hybrid cell line containing a t(1;6)(q21;q13)) translocation chromosome. The hCRABP-II gene is therefore localized to a band known to contain several other genes that are expressed in the context of epidermal differentiation, including profilaggrin, loricrin, involucrin, and calcyclin.
Elder
J T
JT
Department of Dermatology, University of Michigan Medical School, Ann Arbor 48109-0668.
Aström
A
A
Pettersson
U
U
Voorhees
J J
JJ
Trent
J M
JM
D000818:Animals; D002352:Carrier Proteins / Q000235:genetics*; D002454:Cell Differentiation; D002874:Chromosome Mapping / Q000379:methods*; D002878:Chromosomes, Human, Pair 1; D015342:DNA Probes; D005796:Genes / Q000235:genetics*; D006801:Humans; D006822:Hybrid Cells; D015603:Keratinocytes / Q000166:cytology; D051379:Mice; D008856:Microscopy, Fluorescence; D009693:Nucleic Acid Hybridization; D018168:Receptors, Retinoic Acid; D014178:Translocation, Genetic; D014212:Tretinoin
D002352:Carrier Proteins; D015342:DNA Probes; D018168:Receptors, Retinoic Acid; D014212:Tretinoin