Direct detection of Epstein-Barr virus in peripheral blood and comparison of Epstein-Barr virus genotypes present in direct specimens and lymphoblastoid cell lines established from nasopharyngeal carcinoma patients and healthy carriers in Hong Kong.
By means of a PCR assay, EBV was demonstrated directly in peripheral blood of previously infected individuals. The virus was detected in approximately 80% of specimens from EBV-seropositive individuals, but not in cord-blood lymphocytes by this method. When virus present in peripheral blood was compared to that observed directly in NPC biopsies or throat washings, it was distinct from that seen in biopsies in 4/15 cases (27%) and from that seen in throat washes in 1/22 cases (5%). The throat-wash virus differed from the biopsy virus in 3/20 cases (15%). The prototype F virus was found in 7/10 LCLs (70%) established from NPC patients' peripheral blood, but was only detected in 2/9 specimens (22%) directly analyzed by the PCR assay. This finding suggests selective isolation of prototype F EBV in spontaneous LCLs established from NPC patients.
Lung
M L
ML
Department of Microbiology, Queen Mary Hospital, University of Hong Kong.
Lam
W P
WP
Chan
K H
KH
Li
S
S
Sham
J
J
Choy
D
D
D015139:Blotting, Southern; D002353:Carrier State / Q000382:microbiology*; D002460:Cell Line; D004279:DNA, Viral / Q000032:analysis*; D005838:Genotype; D004854:Herpesvirus 4, Human / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D006723:Hong Kong; D006801:Humans; D008214:Lymphocytes / Q000382:microbiology*; D009303:Nasopharyngeal Neoplasms / Q000382:microbiology*; D010614:Pharynx / Q000382:microbiology; D016133:Polymerase Chain Reaction
D004279:DNA, Viral