Syntaxin: a synaptic protein implicated in docking of synaptic vesicles at presynaptic active zones.
Synaptic vesicles store neurotransmitters that are released during calcium-regulated exocytosis. The specificity of neurotransmitter release requires the localization of both synaptic vesicles and calcium channels to the presynaptic active zone. Two 35-kilodalton proteins (p35 or syntaxins) were identified that interact with the synaptic vesicle protein p65 (synaptotagmin). The p35 proteins are expressed only in the nervous system, are 84 percent identical, include carboxyl-terminal membrane anchors, and are concentrated on the plasma membrane at synaptic sites. An antibody to p35 immunoprecipitated solubilized N-type calcium channels. The p35 proteins may function in docking synaptic vesicles near calcium channels at presynaptic active zones.
Bennett
M K
MK
Howard Hughes Medical Institute, Department of Molecular and Cellular Physiology, Stanford University Medical Center, CA 94305.
Calakos
N
N
Scheller
R H
RH
D000595:Amino Acid Sequence; D000818:Animals; D000954:Antigens, Surface; D002135:Calcium-Binding Proteins; D004591:Electrophoresis, Polyacrylamide Gel; D015151:Immunoblotting; D008562:Membrane Glycoproteins / Q000502:physiology; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000302:isolation & purification / Q000502:physiology*; D015345:Oligonucleotide Probes; D051381:Rats; D012689:Sequence Homology, Nucleic Acid; D009435:Synaptic Transmission / Q000502:physiology; D013572:Synaptic Vesicles / Q000502:physiology*; D050863:Synaptotagmin I; D050857:Synaptotagmins; D050827:Syntaxin 1
D000954:Antigens, Surface; D002135:Calcium-Binding Proteins; D008562:Membrane Glycoproteins; D009419:Nerve Tissue Proteins; D015345:Oligonucleotide Probes; D050863:Synaptotagmin I; D050827:Syntaxin 1; D050857:Synaptotagmins