[Quantitative analysis of cytoplasmic myeloperoxidase positive leukemic cells by FCM].
Development of a fixation allowed flow cytometric analysis of nuclear and intracellular antigen in leukemic cells. In this paper the analyzing procedure of cytoplasmic myeloperoxidase by FCM was described. This procedure is more sensitive than cytochemical staining of fixed cell smears and more specific than cell surface immunophenotyping by CD13, CD14, CD33. So, this technique is useful for classification of myelogenous leukemic cells especially MO type leukemia by FAB classification. This technique also allowed two color analysis of cell surface antigens and cytoplasmic antigens. Mixed lineage leukemia can be easily and accurately classified by using of this procedure.
Takahashi
S
S
Dept. of Pediatrics, Faculty of Medicine, Tokyo Medical and Dental University.
Okawa
H
H
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface / Q000032:analysis; D003593:Cytoplasm / Q000276:immunology; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D007951:Leukemia, Myeloid / Q000145:classification* / Q000175:diagnosis; D009195:Peroxidase / Q000032:analysis* / Q000276:immunology
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D009195:Peroxidase