title: Differentiation of oncogenic and nononcogenic strains of Marek's disease virus type 1 by using polymerase chain reaction DNA amplification.
abstract: Differentiation of oncogenic and nononcogenic strains of Marek's disease virus type 1 (MDV1) was attempted by polymerase chain reaction (PCR) using the primers chosen from the sequence within the long inverted repeats of MDV1 DNA. PCR of the DNAs extracted from oncogenic-strain-infected cells and Marek's disease tumor cell lines produced a major product containing two or three copies of 132-base-pair (bp) repeat units, whereas PCRs of the DNAs extracted from nononcogenic-strain-infected cells yielded amplified products with various sizes corresponding to the number of 132-bp repeat units. The primers chosen from the glycoprotein A genes of MDV1 and herpesvirus of turkeys also were used for determination of their serotype specificity. The PCR procedure was found to be a simple and sensitive procedure for identification of MDV1 and herpesvirus of turkeys and for estimation of oncogenicity of MDV1.
authors.affiliation: Department of Cell Regulation, Tokyo Medical and Dental University, Japan.
authors.forename: G S
authors.initials: GS
authors.lastname: Zhu
authors.forename: T
authors.initials: T
authors.lastname: Ojima
authors.forename: T
authors.initials: T
authors.lastname: Hironaka
authors.forename: T
authors.initials: T
authors.lastname: Ihara
authors.forename: N
authors.initials: N
authors.lastname: Mizukoshi
authors.forename: A
authors.initials: A
authors.lastname: Kato
authors.forename: S
authors.initials: S
authors.lastname: Ueda
authors.forename: K
authors.initials: K
authors.lastname: Hirai
mesh_terms: D001483:Base Sequence; D004279:DNA, Viral / Q000235:genetics*; D005814:Genes, Viral / Q000235:genetics; D008381:Herpesvirus 2, Gallid / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics; D012680:Sensitivity and Specificity
chemical_list: D004279:DNA, Viral