title: Molecular and genetic characterization of cytochrome oxidase-negative Aeromonas salmonicida isolated from coho salmon (Oncorhynchus kisutch).
abstract: Cytochrome oxidase variants of the bacterial fish pathogen, Aeromonas salmonicida, were characterized for genetic and molecular homology with cytochrome oxidase-positive isolates that typically induce furunculosis in salmonids. Protein and lipopolysaccharide moieties of the cytochrome oxidase-negative variants were similar to their typical counterparts, based on sodium-dodecyl-sulfate polyacrylamide gel electrophoresis. Pathogenicity of aberrant isolates to brook trout (Salvelinus fontinalis) was similar to typical cytochrome oxidase-positive isolates. Colorimetric deoxyribonucleic acid (DNA) hybridization in 96-well microplates yielded homology values greater than 82.5% for typical aberrant A. salmonicida isolates when photobiotinylated DNA for reference A. salmonicida 3.101 was used as a probe. The only variation of these isolates from typical A. salmonicida was a negative cytochrome oxidase reaction.
authors.affiliation: U.S. Fish and Wildlife Service, National Fish Health Research Laboratory, Kearneysville, West Virginia 25430.
authors.forename: J D
authors.initials: JD
authors.lastname: Teska
authors.forename: R C
authors.initials: RC
authors.lastname: Cipriano
authors.forename: W B
authors.initials: WB
authors.lastname: Schill
mesh_terms: D000333:Aeromonas / Q000145:classification* / Q000201:enzymology / Q000235:genetics / Q000472:pathogenicity; D000818:Animals; D001426:Bacterial Proteins / Q000032:analysis; D004269:DNA, Bacterial / Q000032:analysis; D003576:Electron Transport Complex IV / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D005393:Fish Diseases / Q000382:microbiology*; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology / Q000662:veterinary*; D008070:Lipopolysaccharides / Q000032:analysis; D009693:Nucleic Acid Hybridization; D012474:Salmon / Q000382:microbiology*; D012689:Sequence Homology, Nucleic Acid; D014337:Trout
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D008070:Lipopolysaccharides; D003576:Electron Transport Complex IV