title: Isolation of amine oxidase from bovine plasma by a two-step procedure.
abstract: A novel method for isolation of amine oxidase from bovine plasma is reported; it involves a two-step procedure, namely ammonium sulfate fractionation and affinity chromatography with elution by aniline, which is a competitive inhibitor of the enzyme. A homogeneous enzyme, characterized by a specific activity of 0.44 U/mg, was obtained with a yield higher than 50%.
authors.affiliation: Department of Biological Chemistry, University of Padova, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Vianello
authors.forename: M L
authors.initials: ML
authors.lastname: Di Paolo
authors.forename: L
authors.initials: L
authors.lastname: Zennaro
authors.forename: R
authors.initials: R
authors.lastname: Stevanato
authors.forename: A
authors.initials: A
authors.lastname: Rigo
mesh_terms: D006631:Amine Oxidase (Copper-Containing); D000588:Amines; D000818:Animals; D002417:Cattle; D002846:Chromatography, Affinity; D004578:Electron Spin Resonance Spectroscopy; D004591:Electrophoresis, Polyacrylamide Gel; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000037:antagonists & inhibitors / Q000097:blood* / Q000302:isolation & purification*; D012685:Sepharose / Q000031:analogs & derivatives
chemical_list: D000588:Amines; C024903:aminohexyl-sepharose; D012685:Sepharose; D006631:Amine Oxidase (Copper-Containing); D000587:Oxidoreductases Acting on CH-NH Group Donors