title: Selenoproteins in mitochondria and cytosol of Saccharomyces uvarum after growth in sodium selenite-supplemented media.
abstract: The extent of the biosynthesis of selenoproteins in mitochondria and cytosol of Saccharomyces uvarum depends on the sodium selenite concentration in the medium. In mitochondria there is a selenoprotein (SP 1) exhibiting glutathione peroxidase activity whose concentration already reaches a maximum at low concentrations of sodium selenite. A second selenoprotein (SP 2) was found in mitochondria and cytosol. Both proteins contain L-selenocysteine. The molecular masses of SP 1 and SP 2 are 73,000 Da and 83,000 Da, respectively. A subunit of SP 1 was found to have a molecular mass of 30,000 Da. SP 2, identified as a glycoprotein, has subunits with molecular masses of 36,500 Da and 5,000 Da. The selenium concentration of the total yeast increases from 260 micrograms/kg dry weight to 280 mg/kg dry weight after supplementation of the medium with sodium selenite.
authors.affiliation: Fachrichtung Medizinische Biochemie, Universität des Saarlandes, Homburg, Fed. Rep. of Germany.
authors.forename: H J
authors.initials: HJ
authors.lastname: Haas
authors.forename: M
authors.initials: M
authors.lastname: Velten
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D003470:Culture Media / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D008928:Mitochondria / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism*; D012440:Saccharomyces / Q000254:growth & development / Q000378:metabolism*; D012643:Selenium / Q000378:metabolism*; D051140:Selenoproteins; D018038:Sodium Selenite
chemical_list: D000596:Amino Acids; D003470:Culture Media; D011506:Proteins; D051140:Selenoproteins; D012643:Selenium; D018038:Sodium Selenite