Mucosal protective activity of prostaglandin analogs in rodent colonic inflammation.
The mucosal protective prostaglandin analogs misoprostol, enisoprost, and SC-46275 (the 17E-18-cyclopentenyl analog of enisoprost) were tested in mouse and rat colitis induced by the intrarectal instillation of dilute acetic acid. Colitis was assessed by histology and colonic levels of myeloperoxidase (a neutrophil marker enzyme). When given as enemas 30 min ahead of colitis induction, 15(R)-15-methyl-PGE2 (arbaprostil) and 15(S)-15-methyl-PGE1 were inactive; however, misoprostol, enisoprost, and SC-46275 protected against colonic inflammation with ED50 values of 24, 12 and 1.3 micrograms/kg, respectively, in rats and 11, 5, and 1 micrograms/kg, respectively, in mice. These compounds may have utility in the medical management of human inflammatory bowel disease.
Fretland
D J
DJ
Department of ImmunoInflammatory Diseases Research, Searle Research and Development, Skokie, Illinois 60077.
Widomski
D L
DL
Anglin
C P
CP
Walsh
R E
RE
Levin
S
S
Gasiecki
A F
AF
Collins
P W
PW
D000085:Acetates; D019342:Acetic Acid; D000818:Animals; D000897:Anti-Ulcer Agents / Q000494:pharmacology*; D003092:Colitis / Q000139:chemically induced / Q000188:drug therapy*; D004195:Disease Models, Animal; D007413:Intestinal Mucosa / Q000187:drug effects*; D008297:Male; D015394:Molecular Structure; D009195:Peroxidase / Q000032:analysis; D011465:Prostaglandins, Synthetic / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley
D000085:Acetates; D000897:Anti-Ulcer Agents; D011465:Prostaglandins, Synthetic; D009195:Peroxidase; D019342:Acetic Acid