High performance liquid chromatography of nucleic acids and the related compounds on a fluorinated silica gel column.
Separations of nucleic acids and the related compounds were investigated by HPLC on a new fluorinated bonded silica gel column. Polyadenylate (Poly (A)) enzymatic partial hydrolysate sample and the mixture of various polynucleotide samples were sufficiently separated by the reversed-phase mode using a gradient elution with aqueous ammonium acetate/acetonitrile system. Mixed-mode separation on the fluorinated bonded phase coated with a tert-alkylammonium salt was also examined for the separation of the various polynucleotides including tRNAs.
Itoh
H
H
School of Pharmaceutical Sciences, Kitasato University, Tokyo, Japan.
Kinoshita
T
T
Nimura
N
N
D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation*; D005466:Fluorocarbons / Q000737:chemistry*; D011119:Polynucleotides / Q000302:isolation & purification*; D012343:RNA, Transfer / Q000302:isolation & purification*; D058428:Silica Gel; D012822:Silicon Dioxide / Q000737:chemistry*
D005466:Fluorocarbons; D011119:Polynucleotides; D058428:Silica Gel; D012822:Silicon Dioxide; D012343:RNA, Transfer