Recognition of SV40-VP2 in the infected cell by antipeptide antibodies.
Antipeptide antibodies were elicited against two synthetic peptides corresponding to amino acids 47-55 and 98-103 of the structural protein VP 2 of SV 40. The induced antibodies proved to be VP 2-specific in an immunoblot. In immunofluorescence these antibodies showed a discrete nuclear and perinuclear staining pattern. In immune electron microscopy studies the induced antibodies did not bind to major virions suggesting that VP 2 is not present at the surface of SV 40 particles.
Streckert
H J
HJ
Department of Medical Microbiology and Virology, Ruhr-University Bochum, Federal Republic of Germany.
Sommerfeld
H J
HJ
Morgenroth
K
K
Werchau
H
H
D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology*; D000918:Antibody Specificity; D002213:Capsid / Q000138:chemical synthesis / Q000276:immunology*; D036022:Capsid Proteins; D002460:Cell Line; D005455:Fluorescent Antibody Technique; D016253:Microscopy, Immunoelectron; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology; D011817:Rabbits; D013539:Simian virus 40 / Q000276:immunology* / Q000648:ultrastructure
D000914:Antibodies, Viral; D036022:Capsid Proteins; D010455:Peptides; C442543:VP2 protein, Polyomavirus