Solid-phase extraction and HPLC-UV confirmation of drugs of abuse in urine.
A series of six liquid chromatographic methods were developed to confirm the presence of six classes of drugs of abuse in urine. The chromatographic separations were performed with a reversed-phase C8 column, except in the case of morphine, which was separated on a normal phase column. Isocratic and gradient elutions, ion pair technique, and UV detection were employed. Sample pretreatment involved the extensive application of solid-phase extractions and liquid-liquid extractions on solid supports. The specificity and sensitivity enabled the confirmation of morphine, benzoylecgonine, THC-COOH, amphetamine, and methamphetamine, six barbiturates, and nine benzodiazepines screened positive by EMIT in urine.
Ferrara
S D
SD
Centre of Behavioural and Forensic Toxicology, University of Padova, Italy.
Tedeschi
L
L
Frison
G
G
Castagna
F
F
D000661:Amphetamine / Q000652:urine; D001463:Barbiturates / Q000652:urine; D001569:Benzodiazepines / Q000652:urine; D002851:Chromatography, High Pressure Liquid; D003042:Cocaine / Q000031:analogs & derivatives / Q000652:urine; D013759:Dronabinol / Q000652:urine; D006801:Humans; D013287:Illicit Drugs / Q000652:urine*; D007124:Immunoenzyme Techniques; D009020:Morphine / Q000652:urine; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet; D015813:Substance Abuse Detection / Q000379:methods*
D001463:Barbiturates; D013287:Illicit Drugs; D001569:Benzodiazepines; C005618:benzoylecgonine; D009020:Morphine; D013759:Dronabinol; D000661:Amphetamine; D003042:Cocaine