Involvement of opioid receptors in Met-enkephalin modulation of blast-transformation of mouse splenocytes.
The influence of Met-enkephalin on mitogenic stimulation of mouse splenocytes was investigated. Met-enkephalin (ME) was shown to suppress proliferation induced by Concanavalin A and activate proliferation induced by Staphylococcus enterotoxin A. Both effects were revealed at low (down to 10(-14) M) concentration of pentapeptide. Naloxone reversed ME influence on cell activation. The number of receptors for naloxone was shown to increase up to 2.5-fold during mitogenic activation. The difference in expression of various types of opioid receptors at mitogenic stimulation was demonstrated by ligand displacement experiments.
Zaitsev
S V
SV
Lomonosov State University, Moscow, U.S.S.R.
Khegai
L A
LA
Kim
B B
BB
Gavrilova
E M
EM
Yanovsky
O G
OG
Zakharova
L A
LA
D000818:Animals; D001667:Binding, Competitive; D003208:Concanavalin A / Q000494:pharmacology; D004174:Diprenorphine / Q000378:metabolism / Q000494:pharmacology; D004744:Enkephalin, Methionine / Q000494:pharmacology*; D004768:Enterotoxins / Q000494:pharmacology; D005260:Female; D008213:Lymphocyte Activation / Q000187:drug effects*; D051379:Mice; D008810:Mice, Inbred C57BL / Q000276:immunology; D008808:Mice, Inbred CBA / Q000276:immunology; D009270:Naloxone / Q000494:pharmacology; D010146:Pain / Q000503:physiopathology; D011957:Receptors, Opioid / Q000187:drug effects / Q000502:physiology*; D017465:Receptors, Opioid, delta; D017450:Receptors, Opioid, mu; D012684:Sensory Thresholds; D013154:Spleen / Q000166:cytology
D004768:Enterotoxins; D011957:Receptors, Opioid; D017465:Receptors, Opioid, delta; D017450:Receptors, Opioid, mu; D003208:Concanavalin A; D004174:Diprenorphine; D009270:Naloxone; C026066:enterotoxin A, Staphylococcal; D004744:Enkephalin, Methionine