Rapid determination of human cytomegalovirus susceptibility to ganciclovir directly from clinical specimen primocultures.
We were able to rapidly determine the susceptibility of human cytomegalovirus to ganciclovir using a late antigen synthesis reduction assay directly on primocultures of clinical specimens. This test was compared with a conventional susceptibility assay which was performed with cell-free virus obtained after cytomegalovirus isolation and in vitro passages. Both tests produced similar results. The rapid test, unlike conventional assays, is able to provide a result within 5 days after receipt of the specimen and could thus play a direct role in the therapeutic decision.
Pepin
J M
JM
Department of Virology, Claude Bernard Hospital, Paris, France.
Simon
F
F
Dussault
A
A
Collin
G
G
Dazza
M C
MC
Brun-Vezinet
F
F
D003587:Cytomegalovirus / Q000187:drug effects* / Q000302:isolation & purification; D003586:Cytomegalovirus Infections / Q000188:drug therapy / Q000382:microbiology; D004352:Drug Resistance, Microbial; D005069:Evaluation Studies as Topic; D015774:Ganciclovir / Q000494:pharmacology*; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods*; D013997:Time Factors; D014776:Virus Cultivation
D015774:Ganciclovir