Functional conservation of nematode and vertebrate myogenic regulatory factors.
The Caenorhabditis elegans protein, CeMyoD, is related to the vertebrate myogenic regulatory factors MyoD, myogenin, MRF-4 and Myf-5. Like its vertebrate counterparts, CeMyoD accumulates in the nucleus of striated muscle cells prior to the onset of terminal differentiation. CeMyoD also shares functional similarities with the vertebrate myogenic regulatory factors. Viral LTR driven expression of CeMyoD in mouse 10T1/2 cells can convert this cell line into myoblasts as well as efficiently trans-activate mouse muscle-specific promoters. Furthermore, mouse MyoD expression can activate a CeMyoD-beta-galactosidase reporter construct in a 10T1/2 co-transfection assay.
Krause
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M
Fred Hutchinson Cancer Research Center, Seattle, Washington 98104.
Fire
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A
White-Harrison
S
S
Weintraub
H
H
Tapscott
S
S
D000595:Amino Acid Sequence; D000818:Animals; D016384:Consensus Sequence; D005786:Gene Expression Regulation; D008969:Molecular Sequence Data; D009024:Morphogenesis / Q000235:genetics; D009124:Muscle Proteins; D009132:Muscles / Q000196:embryology*; D017570:MyoD Protein; D011401:Promoter Regions, Genetic; D015534:Trans-Activators
D009124:Muscle Proteins; D017570:MyoD Protein; D015534:Trans-Activators