Anti-tumor necrosis factor receptor and tumor necrosis factor agonist activity by an anti-idiotypic antibody.
Tumor necrosis factor (TNF) is a cytokine which, among other properties, is a principle mediator of inflammation and septic shock. It acts upon target cells by binding to specific cell surface receptors. A10G10 is a murine monoclonal antibody which recognizes human TNF and neutralizes its activity. A rabbit polyclonal antibody directed at the antigen-binding site of A10G10 was raised and affinity purified over an A10G10 column. The resultant anti-idiotypic antibody recognized not only A10G10 but also both TNF receptors. It showed TNF agonist activity in two different TNF bioassays, and competed with several anti-TNF receptor monoclonal antibodies and TNF itself for binding to cells. These results represent an example of a method for obtaining antibodies to a ligand-specific receptor in the absence of the receptor itself.
Galloway
C J
CJ
Miles, Incorporated, (Inc.), Berkeley, Miles, Inc., West Haven.
Madanat
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MS
Sarr
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Espevik
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Dumas
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ML
Mitra
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Ranges
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GE
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000502:physiology*; D006801:Humans; D016328:NF-kappa B / Q000378:metabolism; D011817:Rabbits; D011956:Receptors, Cell Surface / Q000276:immunology*; D018124:Receptors, Tumor Necrosis Factor; D014409:Tumor Necrosis Factor-alpha / Q000502:physiology*
D000888:Antibodies, Anti-Idiotypic; D016328:NF-kappa B; D011956:Receptors, Cell Surface; D018124:Receptors, Tumor Necrosis Factor; D014409:Tumor Necrosis Factor-alpha