Incorporation of BrdU in a DNA fragment may affect protein-DNA interactions in a site-dependent manner.
DNA in which BrdU has been randomly incorporated is used to identify specific DNA-binding proteins. We show here that the location of BrdU incorporation in a DNA fragment can significantly affect specific protein-DNA interactions and thereby identification of DNA-binding proteins. We suggest that a radioactive labeled unmodified DNA, rather than BrdU-incorporated DNA, be used for identifying DNA-binding proteins by UV cross-linking.
Kumar
G
G
Department of Molecular Biology and Genetics, Wayne State University, School of Medicine, Detroit, Michigan 48201.
Sharma
A K
AK
Jayaraman
K
K
D001483:Base Sequence; D001973:Bromodeoxyuridine / Q000494:pharmacology*; D003432:Cross-Linking Reagents; D004279:DNA, Viral / Q000187:drug effects*; D004268:DNA-Binding Proteins / Q000187:drug effects*; D006367:HeLa Cells; D006801:Humans; D007577:JC Virus / Q000235:genetics; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation / Q000187:drug effects; D011401:Promoter Regions, Genetic / Q000187:drug effects; D011485:Protein Binding / Q000187:drug effects; D014466:Ultraviolet Rays
D003432:Cross-Linking Reagents; D004279:DNA, Viral; D004268:DNA-Binding Proteins; D001973:Bromodeoxyuridine