Expression of human 17 beta-hydroxysteroid dehydrogenase in mammalian cells.
The insert of 1278 bp containing the entire coding region of cDNA encoding human 17 beta-hydroxysteroid dehydrogenase (17 beta-HSD) was inserted into a pHS1 vector and expressed in HeLA human cervical carcinoma cells and COS-1 monkey kidney tumor cells. Western blot analysis indicated that the expressed protein migrates at the same position as the purified enzyme and is recognized by the antibody raised against purified human placental 17 beta-HSD. The expressed enzyme efficiently catalyzes the interconversion of estrone and estradiol while dehydroepiandrosterone and 5-androstene-3 beta,17 beta-diol are interconverted at a lower rate. The present data suggest the existence of two 17 beta-HSDs.
Dumont
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M
MRC Group in Molecular Endocrinology, CHUL Research Center, Quebec, Canada.
Luu-The
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V
de Launoit
Y
Y
Labrie
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F
D015067:17-Hydroxysteroid Dehydrogenases / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000235:genetics; D003687:Dehydroepiandrosterone / Q000494:pharmacology; D004958:Estradiol / Q000494:pharmacology; D004970:Estrone / Q000494:pharmacology; D005260:Female; D015870:Gene Expression; D006367:HeLa Cells; D006801:Humans; D007700:Kinetics; D008322:Mammals; D008668:Metallothionein / Q000235:genetics; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D010920:Placenta / Q000201:enzymology; D011247:Pregnancy; D011401:Promoter Regions, Genetic; D015183:Restriction Mapping; D013728:Terminator Regions, Genetic; D014162:Transfection
D009838:Oligodeoxyribonucleotides; D004970:Estrone; D003687:Dehydroepiandrosterone; D004958:Estradiol; D004247:DNA; D008668:Metallothionein; D015067:17-Hydroxysteroid Dehydrogenases; C032690:3-alpha-(17-beta)-hydroxysteroid dehydrogenase (NAD(+))