Wounding acts as a tumor promoter in chickens inoculated with avian sarcoma virus 17.
Avian sarcoma virus 17 (ASV17) is an acutely transforming retrovirus which carries the oncogene v-jun. The virus induces fibrosarcomas in chickens at the site of inoculation. Here we describe wound-related tumor formation in 77% of chickens inoculated with ASV17 in one wing and wounded by metal clip insertion in the opposite wing. Tumors from both wound-related and inoculation-related sites were histologically diagnosed as fibrosarcomas. Tissues cultured from both tumor sites produced infectious virus in culture and expressed high levels of the v-Jun oncoprotein detectable by immunofluorescent staining. By varying the time of wounding relative to virus inoculation we defined the early stages of wound healing (2-7 days postinoculation) as favoring wound-related tumor formation. Three other acutely transforming retroviruses containing oncogenes coding for nonreceptor protein tyrosine kinases (v-src, v-yes, and v-fps), inoculated in the same manner, induced wound-related tumors in all cases. We conclude that in chickens, ASV17 collaborates with wound healing to promote tumorigenesis by a process which may relate either to a biochemical function of Jun or to a more general, shared characteristic of transforming retroviruses.
Marshall
G M
GM
Department of Microbiology, University of Southern California School of Medicine, Los Angeles 90033-1054.
Vanhamme
L
L
Wong
W Y
WY
Su
H
H
Vogt
P K
PK
D000818:Animals; D001358:Avian Sarcoma Viruses / Q000502:physiology*; D002471:Cell Transformation, Neoplastic; D002472:Cell Transformation, Viral; D002645:Chickens; D001357:Sarcoma, Avian / Q000209:etiology* / Q000473:pathology; D014947:Wounds and Injuries / Q000150:complications*