A cyclic AMP response element is involved in retinoic acid-dependent RAR beta 2 promoter activation.
Activation of the retinoic acid receptor (RAR) beta 2 promoter is known to be mediated by a RA response element located in the proximity of the TATA-box. By deletion studies in P19 embryonal carcinoma cells we have analyzed the RAR beta 2 promoter for the presence of additional regulatory elements. We found that the cyclic AMP response element-related motif, TGATGTCA at position -99 to -92, is able to enhance RA-dependent RAR beta 2 promoter activation. In addition we demonstrate that this element, designated CRE-beta 2, is functionally active as a CRE since it can bind members of the CREB/ATF transcription factor family and, moreover, mediates the stimulatory effect of cAMP on RA-dependent RAR beta 2 promoter activation in human foetal kidney 293 cells.
Kruyt
F A
FA
Hubrecht Laboratory, Netherlands Institute for Developmental Biology, Utrecht.
Folkers
G
G
van den Brink
C E
CE
van der Saag
P T
PT
D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D002460:Cell Line; D000242:Cyclic AMP / Q000378:metabolism / Q000494:pharmacology; D017362:Cyclic AMP Response Element-Binding Protein / Q000378:metabolism*; D004247:DNA; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D007668:Kidney / Q000166:cytology / Q000196:embryology; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D011401:Promoter Regions, Genetic; D018168:Receptors, Retinoic Acid; D012045:Regulatory Sequences, Nucleic Acid; D016385:TATA Box; D014212:Tretinoin / Q000378:metabolism*
D002352:Carrier Proteins; D017362:Cyclic AMP Response Element-Binding Protein; D018168:Receptors, Retinoic Acid; D014212:Tretinoin; D004247:DNA; D000242:Cyclic AMP