title: Development of mouse oocytes superovulated at different ages.
abstract: The development of oocytes superovulated at 25, 50, or 90 days in four mouse strains (C57BL/6N, DBA/2N, ICR, and B6D2F1) was examined using the techniques of in vitro fertilization, culture, and transfer of two-cell embryos to pseudopregnant recipients. The highest number of ova were obtained from superovulated 25-day-old mice in all strains. Approximately 80% of oocytes developed to the two-cell stage after in vitro fertilization. Of these living oocytes, 60% developed to weanling stage through the recipient. These results suggested that donor age among 25, 50, or 90-day-old mice has no influence on the viability of superovulated oocytes. Consequently, we conclude that superovulated 25-day-old mice offer an economical and efficient source of viable oocytes for the production of transgenic mice.
authors.affiliation: Laboratory Animal Research Center, University of Tsukuba, Ibaraki, Japan.
authors.forename: F
authors.initials: F
authors.lastname: Sugiyama
authors.forename: N
authors.initials: N
authors.lastname: Kajiwara
authors.forename: S
authors.initials: S
authors.lastname: Hayashi
authors.forename: Y
authors.initials: Y
authors.lastname: Sugiyama
authors.forename: K
authors.initials: K
authors.lastname: Yagami
mesh_terms: D000367:Age Factors; D000818:Animals; D002454:Cell Differentiation; D005260:Female; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D009865:Oocytes / Q000166:cytology*; D013480:Superovulation