Mechanism of the relaxant response of the rat duodenum to bradykinin.
Bradykinin (BK) did not increase cyclic AMP production in cultured rat duodenum smooth muscle cells. Its relaxant effect on the tissue was inhibited by apamin and potentiated by phorbol dibutyrate (PDBU). PDBU also caused a relaxation which was inhibited by apamin. BK's relaxant effect, and its potentiation by PDBU, are due to activation of Ca(2+)-dependent K+ channels.
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Department of Biophysics, Escola Paulista de Medicina, São Paulo, Brazil.
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Paiva
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D000818:Animals; D001920:Bradykinin / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000378:metabolism; D004386:Duodenum / Q000187:drug effects / Q000502:physiology*; D005260:Female; D066298:In Vitro Techniques; D007545:Isoproterenol / Q000494:pharmacology; D008297:Male; D009126:Muscle Relaxation / Q000187:drug effects*; D009130:Muscle, Smooth / Q000187:drug effects / Q000502:physiology*; D015240:Phorbol 12,13-Dibutyrate / Q000494:pharmacology; D015221:Potassium Channels / Q000187:drug effects / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar
D015221:Potassium Channels; D015240:Phorbol 12,13-Dibutyrate; D000242:Cyclic AMP; D007545:Isoproterenol; D001920:Bradykinin; D002118:Calcium