Spectroscopic and biological studies of spin-labeled tetracycline.
A new nitroxyl labeled tetracycline is synthesized. Proton NMR experiments of tetracycline, spin-labeled tetracycline, and the diamagnetic reduced form in DMSO-d6 are reported. The signals observed in the NMR spectra are all assigned. The NMR data revealed that the spin label is attached to the C-2 amide group on ring A of tetracycline. The spin-labeled tetracycline is also tested in vitro for antitumor activity and is found to be active against leukemia P338/ADR cell line and in melanoma LOX cell line.
Pezeshk
A
A
Department of Chemistry, Moorhead State University, Minnesota 56563.
Pezeshk
V
V
D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry* / Q000494:pharmacology; D004578:Electron Spin Resonance Spectroscopy; D007941:Leukemia P388; D009682:Magnetic Resonance Spectroscopy; D008545:Melanoma; D013113:Spin Labels; D013752:Tetracycline / Q000737:chemistry* / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents; D013113:Spin Labels; D013752:Tetracycline