Gene transfer into mammalian central nervous system using herpes virus vectors: extended expression of bacterial lacZ in neurons using the neuron-specific enolase promoter.
A herpes simplex virus (HSV) vector in which the mammalian promoter for neuron-specific enolase (NSE) controls expression of a marker gene was analyzed for its ability to drive expression of this foreign gene in culture and in vivo. In cultured cells, the vector appeared to give neuron-specific expression. Introduction of 10(6) pfu of the virus into the adult rat caudate nucleus by stereotactic injection was not toxic to the animals and yielded beta-galactosidase (beta-gal)-positive neurons for at least 30 days after viral inoculation. This recombinant herpes virus vector is the first described to use a mammalian promoter to yield extended expression of a foreign gene product in the adult mammalian central nervous system (CNS).
Andersen
J K
JK
Neuroscience Center, Massachusetts Hospital East, Charlestown 02129.
Garber
D A
DA
Meaney
C A
CA
Breakefield
X O
XO
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002478:Cells, Cultured; D002490:Central Nervous System / Q000378:metabolism* / Q000382:microbiology; D004277:DNA, Single-Stranded; D005822:Genetic Vectors; D007763:Lac Operon; D008969:Molecular Sequence Data; D009474:Neurons / Q000382:microbiology; D010751:Phosphopyruvate Hydratase / Q000235:genetics*; D011401:Promoter Regions, Genetic; D051381:Rats; D018139:Simplexvirus / Q000235:genetics*; D014162:Transfection
D004277:DNA, Single-Stranded; D010751:Phosphopyruvate Hydratase