title: DNA adducts detected by 32P-postlabelling, in the intestine of rats given bile from patients with familial adenomatous polyposis and from unaffected controls.
abstract: Duodenal adenomas are common in patients with familial adenomatous polyposis (FAP) and cluster around the papilla, suggesting the involvement of bile in their development. Using 32P-postlabelling we determined levels of DNA adducts in the small bowel and colon of rats treated with bile from FAP and control patients. We found a significantly higher level of adducts in the small bowel of rats treated with FAP gallbladder bile compared with control gallbladder bile (P = 0.0034). This result supports the hypothesis that bile plays a role in the development of neoplasia in the foregut of FAP patients.
authors.affiliation: Haddow Laboratory, Royal Marsden Hospital, Sutton, Surrey, UK.
authors.forename: D K
authors.initials: DK
authors.lastname: Scates
authors.forename: A D
authors.initials: AD
authors.lastname: Spigelman
authors.forename: R K
authors.initials: RK
authors.lastname: Phillips
authors.forename: S
authors.initials: S
authors.lastname: Venitt
mesh_terms: D011125:Adenomatous Polyposis Coli / Q000378:metabolism*; D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D001646:Bile / Q000378:metabolism*; D004247:DNA / Q000378:metabolism*; D005260:Female; D006801:Humans; D007422:Intestines / Q000737:chemistry*; D008297:Male; D008795:Metronidazole / Q000494:pharmacology; D008875:Middle Aged; D010761:Phosphorus Radioisotopes; D051381:Rats; D011916:Rats, Inbred F344
chemical_list: D010761:Phosphorus Radioisotopes; D008795:Metronidazole; D004247:DNA