[Hepatitis B virus markers in peripheral blood mononuclear cells].
Recent studies have shown tropism of the hepatitis B virus (HBV) by peripheral blood mononuclear cells (PBMC). The consequences of this phenomenon and their clinical use are not yet clear, however. Seventy-nine patients were studied between March 1989 and October 1990. Sixty-nine patients had chronic liver disease with histological evaluations, and 10 were vaccinated for HBV. The following markers were determined: serum: HBsAg, HBeAg, anti-HBe, antitotal-HBc, anti-HBs, anti-HCV, HBV-DNA; lysated PMBC cells: HBsAg, HBeAg. Hepatic tissue: HBsAg, HBcAg. Four groups were formed according to serology. Group I--positive HBsAg patients (n = 25) HBsAg was observed in the lysated of PBMC in 19 (76%) of the patients. HBeAg in PBMC was detected in 8 (32%), all of them showed evidence of viral replication (presence of HBcAg and/or HBV-DNA in the serum HBcAg in the tissue). Group II--antitotal HBc/anti-HBs positive (n = 14), HBsAg in PBMC was found in 5 (36%) and HBeAg in 1 (7.0%). In this patient replication markers in the serum and in the tissue (HBV-DNA, HBcAg) was also present. Three patients out of 9 anti-HBs positive had HBsAg in PBMC. Group III--seronegative patients for HBV. HBsAg was present in PBMC in 2 (6.6%) of the patients, but was absent in all of them. There was concomitant presence of HBsAg in MN and the hepatic tissue in 1 patient. Replication markers were not observed in the group. Group IV--10 asymptomatic individuals vaccinated for HBV. Except anti-HBs in serum, no other HBV marker could be identified in serum or in PBMC.(ABSTRACT TRUNCATED AT 250 WORDS)
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Serviço de Gastro-Hepatologia, Hospital Universitário Prof. Edgard Santos, Universidade Federal da Bahia, Fiocruz-UFBa.
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Motta
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Carneiro
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Freitas
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D000328:Adult; D015415:Biomarkers / Q000032:analysis; D002908:Chronic Disease; D004279:DNA, Viral / Q000302:isolation & purification; D005260:Female; D006514:Hepatitis B Surface Antigens / Q000032:analysis*; D006515:Hepatitis B virus / Q000235:genetics; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D008107:Liver Diseases / Q000276:immunology*; D008297:Male
D015415:Biomarkers; D004279:DNA, Viral; D006514:Hepatitis B Surface Antigens
Marcadores do vírus da hepatite B em células mononucleares do sangue periférico.