A family outbreak of gastroenteritis caused by group C rotavirus.
A family outbreak of gastroenteritis caused by group C rotavirus is described. All five members of the family, with children between 8 and 15 years of age, fell ill with diarrhea. The diagnosis was initially based on the detection of rotavirus RNA showing a typical group C profile in gel electrophoresis in stool samples, and it was serologically verified from patient sera using a cell culture adapted porcine group C rotavirus as a source of standard antigen. All collected serum samples from the family contained IgM and/or IgG class antibodies to group C rotavirus measurable by immunofluorescence antibody test (IFAT). Group C rotavirus specific IgM class antibodies were present in the early serum samples in 3/4 patients. A roller tube neutralization test (NT) was established to demonstrate neutralizing antibodies to porcine group C rotavirus in human sera. These methods can be used to detect serologically group C rotavirus infections.
Maunula
L
L
Department of Virology, University of Helsinki, Finland.
Svensson
L
L
von Bonsdorff
C H
CH
D000293:Adolescent; D000914:Antibodies, Viral / Q000097:blood; D002648:Child; D003967:Diarrhea / Q000382:microbiology; D004196:Disease Outbreaks; D005243:Feces / Q000382:microbiology; D005260:Female; D005387:Finland / Q000453:epidemiology; D005455:Fluorescent Antibody Technique; D005759:Gastroenteritis / Q000382:microbiology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009500:Neutralization Tests; D012401:Rotavirus / Q000302:isolation & purification / Q000648:ultrastructure; D012400:Rotavirus Infections / Q000382:microbiology*
D000914:Antibodies, Viral