In vitro study of the phagocytic processes in splenic granulocytes of the tench (Tinca tinca, L.).
The different stages of the phagocytic process by splenic granulocytes of Tinca tinca were studied. Adherence capacity to both endothelium and tissue substrate, mobility rate, the phagocytosis capacity for both cells (Candida albicans) and inert particles (latex beads), candidicide power, and capacity of digestion measured by nitroblue tetrazolium (NBT) reduction were evaluated in splenic granulocytes of healthy adult tench. The capacity of adherence to nylon fiber was possessed by 51% of the granulocytes. The percentage capable of adherence to smooth plastic surfaces rose with incubation time. Casein, an effective chemoattractant, increased the random mobility of the granulocytes. Phagocytosis was greater for opsonized C. albicans than for nonopsonized. However, the number of phagocytosed yeast cells destroyed by the granulocytes did not depend on whether or not the C. albicans had been previously opsonized. The phagocytosis indices and the percent phagocytosis of latex beads were greater than those obtained for the phagocytosis of C. albicans in the absence of serum. Finally, the metabolic activity in these cells following the digestion of ingested material showed a 148 +/- 31% stimulation. The results show that splenic cells of tench have the capacity to make a phagocytic response against both cells (C. albicans) and inert particles (latex beads).
Pedrera
I M
IM
Department of Physiology, Faculty of Sciences, University of Extremadura, Badajoz, Spain.
Collazos
M E
ME
Ortega
E
E
Barriga
C
C
D000818:Animals; D002176:Candida albicans / Q000276:immunology; D002448:Cell Adhesion; D002465:Cell Movement; D005260:Female; D005399:Fishes; D006098:Granulocytes / Q000276:immunology*; D066298:In Vitro Techniques; D007840:Latex; D008297:Male; D010587:Phagocytosis / Q000276:immunology*; D013154:Spleen / Q000276:immunology*; D013481:Superoxides / Q000378:metabolism; D013997:Time Factors
D007840:Latex; D013481:Superoxides