title: Association of a cGMP-binding activity with nuclei isolated from amoebae of Dictyostelium discoideum and Polysphondylium violaceum.
abstract: When vegetative and early slug stage amoebae of Dictyostelium discoideum or Polysphondylium violaceum were lysed by filter breakage in a nuclear isolation buffer not containing detergents, substantial levels of a cGMP-binding activity with slow-dissociation kinetics were detected. After fractionation by centrifugation, 50% or more of this binding activity was associated with isolated nuclei. In addition, with Polysphondylium cells, the fraction of stable, nuclear-associated binding activity appeared to increase during cell aggregation. These results support the idea that cGMP might function in the nucleus during early development.
authors.affiliation: Department of Biology, York University, Ont.
authors.forename: J R
authors.initials: JR
authors.lastname: Butler
authors.forename: M B
authors.initials: MB
authors.lastname: Coukell
mesh_terms: D000818:Animals; D002458:Cell Fractionation; D002467:Cell Nucleus / Q000378:metabolism*; D006152:Cyclic GMP / Q000378:metabolism*; D016837:Dictyosteliida / Q000378:metabolism*; D004023:Dictyostelium / Q000378:metabolism*; D007700:Kinetics
chemical_list: D006152:Cyclic GMP