Phenotypic differences between Syrian hamster embryo cells cultured at pH 6.7 or 7.3.
We have investigated the molecular phenotypic differences between Syrian hamster embryo (SHE) cells cultured at pH 6.7 or 7.3. Multiple pH-sensitive phenotypic differences were noted including changes in cellular morphology, a unique charge differential in a major cellular protein, nine uniquely expressed proteins, two unique phosphoserine/threonine phosphoproteins, one unique phosphotyrosine phosphoprotein, and the pH dependent mRNA level of a gap junctional gene (connexin 43). These differences, combined with previously described pH-specific differences (differential transformation rates and gap junctional communication), illustrate that culturing SHE cells in media that differ by 0.6 pH units (0.3 units on either side of pH 7.0) can have a profound influence on the cellular phenotype.
Isfort
R J
RJ
Human and Environmental Safety Division, Procter & Gamble Company, Miami Valley Laboratories, Cincinnati, Ohio 45239.
Cody
D B
DB
Asquith
T N
TN
LeBoeuf
R A
RA
D000818:Animals; D002478:Cells, Cultured; D017630:Connexins; D006224:Cricetinae; D003470:Culture Media; D004622:Embryo, Mammalian / Q000737:chemistry / Q000166:cytology* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007526:Isoelectric Point; D008565:Membrane Proteins / Q000235:genetics*; D008647:Mesocricetus; D008970:Molecular Weight; D010641:Phenotype; D010750:Phosphoproteins / Q000032:analysis / Q000737:chemistry; D011506:Proteins / Q000032:analysis* / Q000737:chemistry; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
D017630:Connexins; D003470:Culture Media; D008565:Membrane Proteins; D010750:Phosphoproteins; D011506:Proteins; D012333:RNA, Messenger