title: The human "peripheral-type" benzodiazepine receptor: regional mapping of the gene and characterization of the receptor expressed from cDNA.
abstract: A cDNA for the human "peripheral-type" benzodiazepine receptor (PBR) was isolated from a liver cDNA library. The 851-nucleotide probe hybridized with a approximately 1 kb mRNA in Northern blots of RNA extracted from various human tissues and cell lines. The human PBR probe was hybridized to DNA from a somatic cell hybrid mapping panel to determine that the gene maps to chromosome 22. With a regional mapping panel for chromosome 22, we localized the gene within band 22q13.31. The ligand-binding properties of the receptor expressed from the cDNA were examined in transient expression experiments and compared to the endogenous human PBR. The PBR ligand [3H]PK 11195 had high affinity for the expressed receptor in COS-1 cells, but the affinities of a pair of isoquinoline propanamide enantiomers differed remarkably in expressed and endogenous human PBR. These findings reveal that the host cell and/or post-translational modification may have an important influence on PBR function.
authors.affiliation: Department of Obstetrics and Gynecology, University of Pennsylvania School of Medicine, Philadelphia 19104.
authors.forename: Y J
authors.initials: YJ
authors.lastname: Chang
authors.forename: R T
authors.initials: RT
authors.lastname: McCabe
authors.forename: H
authors.initials: H
authors.lastname: Rennert
authors.forename: M L
authors.initials: ML
authors.lastname: Budarf
authors.forename: R
authors.initials: R
authors.lastname: Sayegh
authors.forename: B S
authors.initials: BS
authors.lastname: Emanuel
authors.forename: P
authors.initials: P
authors.lastname: Skolnick
authors.forename: J F
authors.initials: JF
authors.lastname: Strauss
mesh_terms: D001483:Base Sequence; D015152:Blotting, Northern; D002460:Cell Line; D002874:Chromosome Mapping; D002892:Chromosomes, Human, Pair 22; D003001:Cloning, Molecular; D004247:DNA; D006801:Humans; D008099:Liver / Q000378:metabolism; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011963:Receptors, GABA-A / Q000235:genetics* / Q000378:metabolism
chemical_list: D011963:Receptors, GABA-A; D004247:DNA