Expression of the gene coding for the major outer capsid protein of SA-11 rotavirus in a baculovirus system.
The gene coding for the major outer capsid protein (VP7) of simian rotavirus SA-11 has been expressed in a baculovirus-insect cell system. The resulting protein is 35 kDa and is primarily associated with the endoplasmic reticulum. Neutralizing SA-11 polyclonal antiserum and VP7 monospecific antiserum reacted specifically with the expressed gene product. Antiserum derived against the recombinant VP7 protein neutralized SA-11 rotavirus infectivity in a fluorescent focus assay.
McGonigal
T P
TP
Department of Corporate Molecular Biology, Abbott Laboratories, North Chicago, IL.
Turon
M C
MC
Komar-Hartnett
K
K
Kister
S E
SE
Smith
R E
RE
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D000956:Antigens, Viral / Q000276:immunology; D016367:Baculoviridae / Q000235:genetics*; D001483:Base Sequence; D002213:Capsid / Q000235:genetics* / Q000276:immunology; D036022:Capsid Proteins; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000737:chemistry; D005814:Genes, Viral; D005822:Genetic Vectors; D006168:Guinea Pigs; D008969:Molecular Sequence Data; D009036:Moths / Q000235:genetics; D009500:Neutralization Tests; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D015183:Restriction Mapping; D012401:Rotavirus / Q000235:genetics* / Q000276:immunology; D015678:Viral Structural Proteins / Q000235:genetics*; D016662:Virus Integration
D000914:Antibodies, Viral; D000956:Antigens, Viral; D036022:Capsid Proteins; D004279:DNA, Viral; D011994:Recombinant Proteins; C050779:VP7 protein, Rotavirus; D015678:Viral Structural Proteins