title: Extracellular pH modifies adaptive response to high K+ in cultured canine kidney cells.
abstract: The chronic interactive and independent effects of extracellular pH and K+ on renal Na(+)-K(+)-adenosinetriphosphatase (ATPase) activity and active K+ transport were studied in the Madin-Darby canine kidney (MDCK) cell line. Confluent cell monolayers were incubated for 24 h in control (4 mM) or high (7.5 mM) K+ medium at acid (6.8) or neutral (7.4) pH. Under acid pH conditions, exposure to high K+ elicited a rise of 133% in maximum Na(+)-K(+)-ATPase activity and 66% in active K+ uptake. In contrast, high K+ had no effect on enzyme activity or K+ uptake at neutral pH. Detergent-activated Na(+)-K(+)-ATPase assay demonstrated a latent pool of enzyme at acid pH-control K+, which seemed to account entirely for the increase in Na(+)-K(+)-ATPase activity after exposure to high K+. The effects of pH appeared unrelated to HCO3- and Cl- concentration in the extracellular environment. We conclude that the upregulatory effect of high K+ on renal Na(+)-K(+)-ATPase is pH dependent. The data suggest that a pool of catalytically inactive enzyme exists only at acid extracellular pH at K+ concentrations in the normal physiological range and that K+ adaptation, at least initially, is the result of recruitment of this latent intracellular pool. In the intact cell extracellular K+ and luminal pH may interact to modify catalytic turnover rate as well as bioavailability of Na(+)-K(+)-ATPase.
authors.affiliation: Department of Radiology, University of Rochester Medical Center, New York 14642.
authors.forename: M A
authors.initials: MA
authors.lastname: Manuli
authors.forename: J M
authors.initials: JM
authors.lastname: Lorenz
mesh_terms: D000222:Adaptation, Physiological; D000818:Animals; D001639:Bicarbonates / Q000494:pharmacology; D001693:Biological Transport, Active / Q000187:drug effects; D002460:Cell Line; D004285:Dogs; D005110:Extracellular Space / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007668:Kidney / Q000166:cytology / Q000378:metabolism*; D011188:Potassium / Q000493:pharmacokinetics / Q000494:pharmacology*; D012964:Sodium / Q000494:pharmacology; D017693:Sodium Bicarbonate; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D012996:Solutions
chemical_list: D001639:Bicarbonates; D012996:Solutions; D012967:Sodium Dodecyl Sulfate; D017693:Sodium Bicarbonate; D012964:Sodium; D000254:Sodium-Potassium-Exchanging ATPase; D011188:Potassium