title: Activation of muscarinic cholinergic receptors stimulates inositol phosphates synthesis in the developing avian cochlear duct.
abstract: We previously reported that the inositol phosphates (IPs) synthesis is induced by muscarinic agonists in the rat cochlea and that this stimulation is maximal at postnatal day 12. This peak response is concomitant with the onset of the efferent synaptogenesis at the outer hair cell level. Whether the correlation between this neuronal plasticity and the enhanced IPs formation is unique to the rat or a general feature of the developing vertebrate cochlea is not known. To examine this question, we measured, in the presence of LiCl, the accumulation of (3H)-IPs induced by carbachol, in the developing chick cochlear duct during a period ranging from embryonic day (E) 8 to post-hatching day (P) 20. Carbachol (1 mM) causes a significant increase of IPs formation relative to basal values at all ages. This IPs accumulation is maximal at E8 (1854% of the basal level), then, rapidly decreases until P13 when it reaches a steady-state level of 294% of the basal level. Strikingly, this gradual decline in IPs formation is interrupted between E15 and E19, by a transient increase in IPs synthesis. This rise peaks at E16 with a stimulation value of 757% of the control level. This maximal stimulation is inhibited by atropine in a dose-dependent manner, as is the case at E9, suggesting the involvement of muscarinic receptors. Interestingly, the occurrence of the peak response is concomitant with the plastic events associated with the maturation of the efferent innervation of the cochlear duct. Thus, these results suggest that there may be a correlation between cochlear plasticity and enhanced IPs synthesis, which is not species-specific.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: INSERM-U 254, Laboratoire de Neurobiologie de l'Audition, Hôpital St Charles, Montpellier, France.
authors.forename: S
authors.initials: S
authors.lastname: Bartolami
authors.forename: E
authors.initials: E
authors.lastname: Mayat
authors.forename: W R
authors.initials: WR
authors.lastname: Lippe
authors.forename: G
authors.initials: G
authors.lastname: Rebillard
authors.forename: R
authors.initials: R
authors.lastname: Pujol
mesh_terms: D000818:Animals; D001285:Atropine / Q000494:pharmacology; D001711:Biotransformation / Q000187:drug effects; D002217:Carbachol / Q000494:pharmacology; D002642:Chick Embryo; D002712:Chlorides / Q000494:pharmacology; D003053:Cochlear Duct / Q000187:drug effects / Q000378:metabolism*; D007295:Inositol Phosphates / Q000096:biosynthesis*; D008094:Lithium / Q000494:pharmacology; D018021:Lithium Chloride; D010277:Parasympathomimetics / Q000494:pharmacology; D011976:Receptors, Muscarinic / Q000187:drug effects / Q000502:physiology*
chemical_list: D002712:Chlorides; D007295:Inositol Phosphates; D010277:Parasympathomimetics; D011976:Receptors, Muscarinic; D001285:Atropine; D002217:Carbachol; D008094:Lithium; D018021:Lithium Chloride