title: Differential expression of subspecies of polyomavirus and murine leukemia virus enhancer core binding protein, PEBP2, in various hematopoietic cells.
abstract: The core sequence of the enhancer of murine leukemia virus (MuLV) long terminal repeat is highly conserved in a large number of MuLV strains and appears to play an essential role when SL3-3 or Moloney strains induce T cell lymphoma in mice. We found by using the electrophoretic mobility shift assay that a polyomavirus enhancer core-binding protein, PEBP2, bound to this core motif of MuLV. We also noted that PEBP2 in several hematopoietic cell lines derived from B lymphocyte, macrophage and myelocyte lineages migrated significantly faster than the authentic PEBP2 detected in NIH3T3 fibroblasts. Interestingly, PEBP2 detected in the cell lines of T lymphocyte lineage appeared to contain both types, which were indistinguishable in electrophoretic mobility from those of NIH3T3 and of B lymphocyte, macrophage and myelocyte lineages. The treatment of the nuclear extract containing PEBP2 with phosphatase generated PEBP3, which is a subcomponent of PEBP2 and retained the same DNA-binding specificity as PEBP2. The altered mobility of hematopoietic cell-derived or T lymphocyte-derived PEBP2 was found to be due to the alteration of the mobility of PEBP3. Based on the distinct mobility of PEBP2/3 of T lymphocytes from those of other hematopoietic cells, we discuss the implication of PEBP2 in MuLV-induced T cell leukemia and T cell-specific gene expression.
authors.affiliation: Department of Viral Oncology, Kyoto University.
authors.forename: M
authors.initials: M
authors.lastname: Satake
authors.forename: M
authors.initials: M
authors.lastname: Inuzuka
authors.forename: K
authors.initials: K
authors.lastname: Shigesada
authors.forename: T
authors.initials: T
authors.lastname: Oikawa
authors.forename: Y
authors.initials: Y
authors.lastname: Ito
mesh_terms: D016475:3T3 Cells / Q000382:microbiology / Q000502:physiology; D000135:Acid Phosphatase / Q000494:pharmacology; D000818:Animals; D001402:B-Lymphocytes / Q000382:microbiology / Q000502:physiology; D001483:Base Sequence; D001665:Binding Sites; D050659:Core Binding Factor Alpha 1 Subunit; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D004586:Electrophoresis; D004742:Enhancer Elements, Genetic / Q000235:genetics* / Q000502:physiology; D015870:Gene Expression / Q000235:genetics; D014644:Genetic Variation / Q000235:genetics; D006413:Hematopoietic System / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D007939:Leukemia L1210 / Q000235:genetics / Q000382:microbiology / Q000473:pathology; D051379:Mice; D008969:Molecular Sequence Data; D008979:Moloney murine leukemia virus / Q000235:genetics* / Q000378:metabolism; D011120:Polyomavirus / Q000235:genetics* / Q000378:metabolism; D013601:T-Lymphocytes / Q000382:microbiology / Q000502:physiology; D050656:Transcription Factor AP-2; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D014758:Viral Core Proteins / Q000235:genetics* / Q000378:metabolism / Q000502:physiology
chemical_list: D050659:Core Binding Factor Alpha 1 Subunit; D004268:DNA-Binding Proteins; C493716:Runx2 protein, mouse; D050656:Transcription Factor AP-2; D014157:Transcription Factors; D014758:Viral Core Proteins; C060759:enhancer-binding protein AP-3; D000135:Acid Phosphatase