[Blood leukotriene B4 response to endotoxin in fish oil fed rats].
Six-week-old male Wistar rats were given a low fat diet containing 15% cod liver oil (FO; n = 30) or 15% safflower oil (SO; n = 30) for 6 weeks. In the polymorphonuclear leukocytes, arachidonic acid (AA) was significantly lower in the FO group than in the SO group (p < 0.001), and eicosapentaenoic acid (EPA) in the FO group was present in large amounts showing the EPA/AA ratio of 1.76. When lipopolysaccharide (LPS; 10mg/kg) was intraperitoneally injected, white blood cell counts in the blood significantly decreased in both groups (p < 0.001) compared to the controls 2 hours later, and at 4 hours, the counts in the SO group (2,033 +/- 151/mm3) were lower than in the FO group (3,189 +/- 624/mm3), (p < 0.01). In both groups, leukotriene B4 (LTB4) in the whole blood increased at 4 hours following LPS-administration compared to the controls (1,219 +/- 167.3pg/ml in the SO group and 600.0 +/- 109.0pg/ml in the FO group, p < 0.001). It should be noted that the level of LTB4 in the SO group was significantly higher than in the FO group (p < 0.001). Changes of the metabolites including decreased LTB4 production in the arachidonic cascade related to the increase of EPA may play a role in the inhibition of the decrease in white blood cell counts.
Urata
T
T
Second Department of Surgery, Kinki University School of Medicine, Sayama, Japan.
Nakatani
M
M
Imano
M
M
Tomiyoshi
H
H
Sonobe
N
N
Yamada
Y
Y
Kasahara
Y
Y
D000284:Administration, Oral; D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism; D003060:Cod Liver Oil / Q000008:administration & dosage / Q000494:pharmacology*; D015118:Eicosapentaenoic Acid / Q000378:metabolism; D004731:Endotoxins / Q000009:adverse effects*; D007958:Leukocyte Count / Q000187:drug effects; D007975:Leukotriene B4 / Q000097:blood*; D008070:Lipopolysaccharides / Q000009:adverse effects; D008297:Male; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012450:Safflower Oil / Q000008:administration & dosage / Q000494:pharmacology
D004731:Endotoxins; D008070:Lipopolysaccharides; D007975:Leukotriene B4; D016718:Arachidonic Acid; D012450:Safflower Oil; D003060:Cod Liver Oil; D015118:Eicosapentaenoic Acid