title: Human papillomavirus detection in cervical cells by in situ hybridization with biotinylated probes.
abstract: We have investigated the applicability of human papillomavirus (HPV) DNA detection by in situ hybridization with biotinylated probes in epithelial cells obtained from the cervix using a cotton tip swab. We describe a simple procedure for obtaining homogeneous cell samples and good preservation of cellular structure. This is achieved by pretreatment of cells with L-cysteine before hybridization. Separate denaturation of cellular DNA and probe DNA is also necessary for satisfactory results. Both benign HPV DNA 6/11 and potentially oncogenic HPV DNA 16/18 could be identified in our series. In situ hybridization on cervical scrapes is a rapid, simple and very specific method for detecting patients infected with oncogenic HPV types.
authors.affiliation: INSERM U346, Affiliée CNRS, Pav. R, Hôpital E. Herriot, Lyon, France.
authors.forename: Y
authors.initials: Y
authors.lastname: Chardonnet
authors.forename: F
authors.initials: F
authors.lastname: Béjui-Thivolet
authors.forename: I
authors.initials: I
authors.lastname: Guérin-Reverchon
mesh_terms: D000328:Adult; D001710:Biotin; D015139:Blotting, Southern; D002460:Cell Line; D002584:Cervix Uteri / Q000166:cytology / Q000382:microbiology*; D015344:DNA Probes, HPV; D005260:Female; D006367:HeLa Cells; D006801:Humans; D017403:In Situ Hybridization; D008875:Middle Aged; D027383:Papillomaviridae / Q000302:isolation & purification*; D014626:Vaginal Smears / Q000379:methods*
chemical_list: D015344:DNA Probes, HPV; D001710:Biotin