title: Regulation of IL-6 receptor and gp130 expression on human cell lines of lymphoid and myeloid origin.
abstract: The expression of 80 kDa interleukin-6 receptor (IL-6R) and the associated molecule gp130 has been studied on human cell lines by FACS- and Northern blot analysis. The effects of dexamethasone, dibutyric-(DB)-cAMP and phorbol-12-myristate-13-acetate (TPA) have been studied on plasmacytoma cell line U266, B cell line BMNH and monocytoid cell line U937. Our data show a definite downregulation of IL-6R and gp130 expression by TPA in U266 and BMNH at both mRNA and cell surface protein levels. In U937 TPA inhibits only the IL-6R expression, without affecting that of gp130. DB-cAMP decreases the expression of both proteins in U937, slightly inhibits the IL-6R expression in U266, but is uneffective in BMNH. Dexamethasone induces considerable upregulation of gp130 only in U266. Our findings suggest separate regulation of IL-6R and gp130 on U266, BMNH and U937 cell lines.
authors.affiliation: Department of Molecular Biology, National Institute of Rheumatology and Physiotherapy, Budapest, Hungary.
authors.forename: A
authors.initials: A
authors.lastname: Falus
authors.forename: T
authors.initials: T
authors.lastname: Taga
authors.forename: M
authors.initials: M
authors.lastname: Hibi
authors.forename: M
authors.initials: M
authors.lastname: Murakami
authors.forename: T
authors.initials: T
authors.lastname: Kishimoto
mesh_terms: D015703:Antigens, CD; D001402:B-Lymphocytes / Q000187:drug effects / Q000276:immunology; D003994:Bucladesine / Q000494:pharmacology; D002460:Cell Line; D050822:Cytokine Receptor gp130; D003907:Dexamethasone / Q000494:pharmacology; D015536:Down-Regulation; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism*; D009000:Monocytes / Q000187:drug effects / Q000276:immunology; D010950:Plasma Cells / Q000187:drug effects / Q000276:immunology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011971:Receptors, Immunologic / Q000235:genetics / Q000378:metabolism*; D019947:Receptors, Interleukin-6; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D015854:Up-Regulation
chemical_list: D015703:Antigens, CD; C494199:IL6ST protein, human; D015850:Interleukin-6; D008562:Membrane Glycoproteins; D012333:RNA, Messenger; D011971:Receptors, Immunologic; D019947:Receptors, Interleukin-6; D050822:Cytokine Receptor gp130; D003994:Bucladesine; D003907:Dexamethasone; D013755:Tetradecanoylphorbol Acetate