Phosphatidylcholine metabolism in ischemic and hypoxic hearts.
The rates of phosphatidylcholine biosynthesis in the isolated hamster hearts under ischemic and hypoxic conditions were examined. Global ischemia was produced by perfusion of the heart with a reduced flow, whereas hypoxia was produced by perfusion with a N2-saturated buffer. A 51% reduction in the biosynthesis of phosphatidylcholine was observed in the ischemic heart. The reduction was caused by a severe decrease in ATP level which resulted in a diminished conversion of choline into phosphocholine. A 22% reduction in the biosynthetic rate of phosphatidylcholine was also detected in the hypoxic heart. The reduction was caused by a diminished level of CTP which resulted in a decreased conversion of phosphocholine to CDP-choline. No compensatory mechanism was triggered during ischemia, but the CTP: phosphocholine cytidylyltransferase activity was enhanced in the hypoxic heart. Our results demonstrate the possible rate-limiting role of choline kinase and reconfirm the regulatory role of the cytidylyltransferase in the biosynthesis of phosphatidylcholine.
Choy
P C
PC
Department of Biochemistry & Molecular Biology, Faculty of Medicine, University of Manitoba, Winnipeg, Canada.
Chan
M
M
Hatch
G
G
Man
R Y
RY
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001692:Biological Transport; D015687:Cell Hypoxia; D002794:Choline / Q000378:metabolism; D002797:Choline Kinase / Q000378:metabolism; D019916:Choline-Phosphate Cytidylyltransferase; D006224:Cricetinae; D003566:Cytidine Diphosphate Choline / Q000378:metabolism; D003570:Cytidine Triphosphate / Q000378:metabolism; D008647:Mesocricetus; D017202:Myocardial Ischemia / Q000378:metabolism*; D009713:Nucleotidyltransferases / Q000378:metabolism; D010713:Phosphatidylcholines / Q000378:metabolism*; D010767:Phosphorylcholine / Q000378:metabolism
D010713:Phosphatidylcholines; D010767:Phosphorylcholine; D003566:Cytidine Diphosphate Choline; D003570:Cytidine Triphosphate; D000255:Adenosine Triphosphate; D002797:Choline Kinase; D009713:Nucleotidyltransferases; D019916:Choline-Phosphate Cytidylyltransferase; D002794:Choline