title: Changes of drebrin expression in the visual cortex of the cat during development.
abstract: The expression of and developmental changes in drebrin were studied in cat visual cortex using immunohistochemistry and immunoblot analysis. Drebrin is a developmentally regulated brain protein which in the chicken has characteristic changes in expression related to developmental stage. A monoclonal antibody (MAb M2F6) raised against drebrin, was found to label the neuropil of the kitten visual cortex in the early postnatal period. At 1-3 weeks of age, the staining was prominent in layer IV of the visual cortex. The immunoreactivity, however, was found to be dramatically decreased around the end of the sensitive period for ocular dominance plasticity (approximately 3 months of age). In the adult visual cortex, almost no immunostaining was observed. These developmental changes revealed by an immunohistochemical method were confirmed using immunoblot analysis. Upon immunoblot analysis after SDS-PAGE of protein from the kitten visual cortex, MAb M2F6 was found to recognize two protein bands with molecular weights of 130 kDa (drebrin E) and 140 kDa (drebrin A). The developmental profile of the intensity of the two bands of the drebin closely parallels in time the postnatal changes in cortical susceptibility to visual deprivation. These results indicate that the expression of drebrin in kitten visual cortex is restricted to the early postnatal period and suggest that it may play an important role in the experience-dependent modification of cortical circuitry during the sensitive period.
authors.affiliation: Department of Neuroscience, Osaka Bioscience Institute, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Imamura
authors.forename: T
authors.initials: T
authors.lastname: Shirao
authors.forename: K
authors.initials: K
authors.lastname: Mori
authors.forename: K
authors.initials: K
authors.lastname: Obata
mesh_terms: D000375:Aging / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn; D000911:Antibodies, Monoclonal; D002415:Cats; D007150:Immunohistochemistry; D009473:Neuronal Plasticity; D009479:Neuropeptides / Q000378:metabolism*; D013997:Time Factors; D014793:Visual Cortex / Q000254:growth & development / Q000378:metabolism*
chemical_list: D000911:Antibodies, Monoclonal; D009479:Neuropeptides; C053386:drebrins