Hydroxyl radical generation in the NADH/microsomal reduction of vanadate.
ESR spin trapping measurements demonstrate generation of hydroxyl (.OH) radical from reduction of vanadate by rat liver microsomes/NADH without exogenous H2O2. Catalase decreases the .OH signal while increasing a vanadium (4+) signal. Addition of superoxide dismutase (SOD) or measurements under an argon atmosphere show decreased .OH radical production. The results suggest that during the one-electron vanadate reduction process by microsomes/NADH, molecular oxygen is reduced to H2O2, which then reacts with vanadium (4+) to generate .OH radical via a Fenton-like mechanism.
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Department of Chemistry, West Virginia University, Morgantown 26506.
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D000818:Animals; D002374:Catalase / Q000378:metabolism; D004578:Electron Spin Resonance Spectroscopy; D005609:Free Radicals; D006861:Hydrogen Peroxide / Q000378:metabolism; D006878:Hydroxides / Q000378:metabolism*; D017665:Hydroxyl Radical; D008862:Microsomes, Liver / Q000378:metabolism*; D008956:Models, Chemical; D009243:NAD / Q000378:metabolism*; D010084:Oxidation-Reduction; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000378:metabolism; D014638:Vanadates / Q000378:metabolism*
D005609:Free Radicals; D006878:Hydroxides; D009243:NAD; D017665:Hydroxyl Radical; D014638:Vanadates; D006861:Hydrogen Peroxide; D002374:Catalase; D013482:Superoxide Dismutase