Detection and typing of human papillomaviruses present in fixed and stained archival cervical smears by a consensus polymerase chain reaction and direct sequence analysis allow the identification of a broad spectrum of human papillomavirus types.
DNA well suited for polymerase chain reaction (PCR) amplification was purified from archival Papanicolaou smears. The detection of a wide range of human papillomavirus (HPV) types was made possible using a HPV-specific consensus primer pair, and typing was conveniently done by direct sequence analysis of the PCR product. The method could be of unique value in longitudinal and cross-sectional studies aimed at answering a number of fundamental pathological and epidemiological questions regarding HPV infection of the genital tract.
Smits
H L
HL
Department of Virology, Section of Fundamental Virology), University of Amsterdam, The Netherlands.
Tieben
L M
LM
Tjong-A-Hung
S P
SP
Jebbink
M F
MF
Minnaar
R P
RP
Jansen
C L
CL
ter Schegget
J
J
D001483:Base Sequence; D002584:Cervix Uteri / Q000382:microbiology; D004279:DNA, Viral / Q000032:analysis* / Q000235:genetics; D005260:Female; D006801:Humans; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D065006:Papanicolaou Test; D027383:Papillomaviridae / Q000145:classification / Q000235:genetics*; D016133:Polymerase Chain Reaction; D014412:Tumor Virus Infections / Q000175:diagnosis* / Q000235:genetics; D014626:Vaginal Smears
D004279:DNA, Viral; D009838:Oligodeoxyribonucleotides