Block of Ca2+ wave and Ca2+ oscillation by antibody to the inositol 1,4,5-trisphosphate receptor in fertilized hamster eggs.
The concentration of cytoplasmic free calcium (Ca2+) increases in various stimulated cells in a wave (Ca2+ wave) and in periodic transients (Ca2+ oscillations). These phenomena are explained by inositol 1,4,5-trisphosphate (IP3)-induced Ca2+ release (IICR) and Ca(2+)-induced Ca2+ release (CICR) from separate intracellular stores, but decisive evidence is lacking. A monoclonal antibody to the IP3 receptor inhibited both IICR and CICR upon injection of IP3 and Ca2+ into hamster eggs, respectively. The antibody completely blocked sperm-induced Ca2+ waves and Ca2+ oscillations. The results indicate that Ca2+ release in fertilized hamster eggs is mediated solely by the IP3 receptor, and Ca(2+)-sensitized IICR, but not CICR, generates Ca2+ waves and Ca2+ oscillations.
Miyazaki
S
S
Department of Physiology, Tokyo Women's Medical College, Japan.
Yuzaki
M
M
Nakada
K
K
Shirakawa
H
H
Nakanishi
S
S
Nakade
S
S
Mikoshiba
K
K
D000818:Animals; D000911:Antibodies, Monoclonal; D002110:Caffeine / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D015220:Calcium Channels; D006224:Cricetinae; D004305:Dose-Response Relationship, Drug; D005306:Fertilization / Q000502:physiology*; D015151:Immunoblotting; D053496:Inositol 1,4,5-Trisphosphate Receptors; D008297:Male; D010063:Ovum / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000187:drug effects / Q000502:physiology*; D018160:Receptors, Cytoplasmic and Nuclear; D012433:Ryanodine / Q000494:pharmacology; D013094:Spermatozoa / Q000502:physiology; D013997:Time Factors
D000911:Antibodies, Monoclonal; D015220:Calcium Channels; D053496:Inositol 1,4,5-Trisphosphate Receptors; D011956:Receptors, Cell Surface; D018160:Receptors, Cytoplasmic and Nuclear; D012433:Ryanodine; D002110:Caffeine; D002118:Calcium