Characterization of EBV-positive lymphoblastoid cell lines obtained from HIV seropositive patients with or without lymphomas.
Epstein-Barr-virus- (EBV-) positive lymphoblastoid cell lines (LCLs) spontaneously arising in vitro were obtained from the peripheral blood of six HIV-seropositive patients and from the peripheral blood and the bone marrow of one patient (LAM) with AIDS and lymphoma. The LCLs from HIV-seropositive patients had phenotypic, cytogenetic, and biological characteristics indistinguishable from those of normal LCLs obtained by infecting B cells with EBV in vitro. The LCLs from LAM patient comprised composite cell populations. Cloning analysis and cell fractionation procedures showed that, beside normal EBV-infected cells, these lines contained a malignant subset population characterized by c-myc rearrangement, abnormal karyotype, and a surface phenotype similar to that of Burkitt's lymphoma cells. Analyses of Ig heavy chain and c-myc oncogene loci showed that these malignant cells were the progeny of a single precursor. Nevertheless, these cells had heterogeneous EBV-fused termini, a finding which indicates that EBV infection followed c-myc rearrangement.
Roncella
S
S
Istituto Nazionale per la Ricerca sul Cancro, IST, Genova, Italy.
Di Celle
P F
PF
Cutrona
G
G
Carbone
A
A
Sessarego
M
M
Dodi
F
F
Foa
R
R
Rowe
M
M
Ferrarini
M
M
D015139:Blotting, Southern; D002051:Burkitt Lymphoma / Q000235:genetics / Q000276:immunology*; D002460:Cell Line; D015321:Gene Rearrangement; D006679:HIV Seropositivity / Q000097:blood*; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000276:immunology*; D006801:Humans; D007075:Immunoglobulin M / Q000032:analysis* / Q000235:genetics; D008214:Lymphocytes / Q000276:immunology*; D014178:Translocation, Genetic
D007075:Immunoglobulin M