[Studies on the effects of rhTNF-alpha anti-replication against EHFV and HAV in vitro].
It was demonstrated in this study that rhTNF-alpha can inhibit the replication of epidemic hemorrhagic fever virus and hepatitis A virus in tissue culture. EHFV infection produced strong fluorescence ( - +) on Vero-E6 cells after 9 days. In the rhTNF-alpha treated group, however the fluorescence was very weak (- or +/-). rhTNF-alpha also showed anti-HAV effects on 2BS cells. The fluorescence intensity on 2BS cells infected by HAV was - +, but in the rhTNF-alpha treated group, the fluorescence was negative. We observed that rhTNF-alpha inhibited EHFV antigen expression on Vero-E6 at different times (-2h, 0h, +2h). Dr. Old had recently suggested that the physiological role of rhTNF-alpha is that of a protective agent against infectious diseases, and this view is supported by our findings.
Jia
F
F
Institute of Hepatology, Beijing Army General Hospital.
D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D002478:Cells, Cultured; D006476:Orthohantavirus / Q000502:physiology*; D006507:Hepatovirus / Q000502:physiology*; D006801:Humans; D008168:Lung / Q000166:cytology; D011994:Recombinant Proteins / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*; D014709:Vero Cells; D014779:Virus Replication / Q000187:drug effects*
D000998:Antiviral Agents; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha