title: A protein of the endoplasmic reticulum involved early in polypeptide translocation.
abstract: To identify components of the mammalian endoplasmic reticulum involved in the translocation of secretory proteins, crosslinking and reconstitution methods were combined. A multispanning abundant membrane glycoprotein was found which is in proximity to nascent chains early in translocation. In reconstituted proteoliposomes, this protein is stimulatory or required for the translocation of secretory proteins.
authors.affiliation: Max-Delbrück Center for Molecular Medicine, Berlin-Buch, Germany.
authors.forename: D
authors.initials: D
authors.lastname: Görlich
authors.forename: E
authors.initials: E
authors.lastname: Hartmann
authors.forename: S
authors.initials: S
authors.lastname: Prehn
authors.forename: T A
authors.initials: TA
authors.lastname: Rapoport
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001692:Biological Transport / Q000502:physiology; D002135:Calcium-Binding Proteins; D003432:Cross-Linking Reagents; D004285:Dogs; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D006801:Humans; D008562:Membrane Glycoproteins / Q000737:chemistry* / Q000302:isolation & purification / Q000502:physiology*; D026901:Membrane Transport Proteins; D008861:Microsomes / Q000378:metabolism; D008969:Molecular Sequence Data; D011506:Proteins / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000502:physiology; D018160:Receptors, Cytoplasmic and Nuclear; D018000:Receptors, Peptide
chemical_list: D002135:Calcium-Binding Proteins; D003432:Cross-Linking Reagents; D008562:Membrane Glycoproteins; D026901:Membrane Transport Proteins; D011506:Proteins; D011956:Receptors, Cell Surface; D018160:Receptors, Cytoplasmic and Nuclear; D018000:Receptors, Peptide; C486174:TRAM1 protein, human; C066973:signal sequence receptor; C074282:translocating chain-associating membrane protein (TRAM)