Production of chromogranin A and B derived peptides in human small cell lung carcinoma cell lines.
Production of chromogranin (Cg)A and B derived peptides [pancreastatin (PST), GAWK, CCB] was studied using human lung carcinoma derived cell lines. PST-like immunoreactivity (LI) was detected in the culture medium in 3 of 6 small cell lung carcinoma (SCLC) cell lines, while GAWK- and CCB-LIs were detected in 5 of 6 and all the 6 SCLC cell lines, respectively. CCB-LI was produced in large amounts in SCLC cell lines as compared to PST- and GAWK-LIs. In non-SCLC cell lines, on the other hand, PST- and GAWK-LIs were not detected. CCB-LI was detected in 1 of 7 non-SCLC cell lines, but not detected in the remainder. PST, GAWK and CCB-LIs, secreted by these cell lines, consisted of several peaks, and these peaks were different among cell lines. This suggests that processing of CgA and B is different in the cell lines. Production of CgA and B derived peptides seems to be a characteristic feature of SCLC, and among them, CCB LI may be a useful marker for SCLC.
Iguchi
H
H
Department of Biochemistry, National Kyushu Cancer Center, Fukuoka, Japan.
Bannai
S
S
Takanashi
N
N
Tsukada
Y
Y
D018288:Carcinoma, Small Cell / Q000378:metabolism*; D002850:Chromatography, Gel; D053379:Chromogranin A; D002864:Chromogranins / Q000378:metabolism*; D003470:Culture Media; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism*; D009363:Neoplasm Proteins / Q000096:biosynthesis*; D010452:Peptide Biosynthesis; D010446:Peptide Fragments / Q000096:biosynthesis*; D014407:Tumor Cells, Cultured / Q000378:metabolism
C506150:CHGA protein, human; D053379:Chromogranin A; D002864:Chromogranins; D003470:Culture Media; D009363:Neoplasm Proteins; D010446:Peptide Fragments