title: Evaluation of desferal as a bifunctional chelating agent for labeling antibodies with Zr-89.
abstract: Zirconium-desferal was prepared and analysed by TLC, NMR and u.v.-spectroscopy. The stoichiometry of the complex was found to be 1:1. Chelation of desferal, coupled to resin, with 88Zr appeared to be fast and almost quantitative in various buffer systems in a broad pH-range (4-7). A high in vitro stability of the zirconium-desferal complex was observed; less than 0.2% zirconium was lost within 24 h in plasma-solutions.
authors.affiliation: Radio-Nuclide-Centre (RNC), Free University, Amsterdam, The Netherlands.
authors.forename: W E
authors.initials: WE
authors.lastname: Meijs
authors.forename: J D
authors.initials: JD
authors.lastname: Herscheid
authors.forename: H J
authors.initials: HJ
authors.lastname: Haisma
authors.forename: H M
authors.initials: HM
authors.lastname: Pinedo
mesh_terms: D000906:Antibodies; D002614:Chelating Agents; D003676:Deferoxamine; D005069:Evaluation Studies as Topic; D007553:Isotope Labeling / Q000379:methods*; D011868:Radioisotopes; D015040:Zirconium
chemical_list: D000906:Antibodies; D002614:Chelating Agents; D011868:Radioisotopes; D015040:Zirconium; D003676:Deferoxamine