Tonin-like activity present in the human submandibular gland.
An enzyme which is able to liberate angiotensin II from angiotensin I, angiotensinogen(1-14) fragment and angiotensinogen was purified from human submandibular gland. Its molecular weight is 110,000; is inhibited by PMSF but not by EDTA or enalaprilat. The pH optima for angiotensin II liberation were 4.0 for angiotensin I, 7.0 for angiotensinogen(1-14) fragment and 8.0 for angiotensinogen. The total amount of angiotensin II generating activity in the human submandibular gland is 5,000-times smaller than that in the rat gland.
Gualberto
M P
MP
Department of Physiology and Biophysics, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
Nunes
R L
RL
Beraldo
W T
WT
Pesquero
J L
JL
D000595:Amino Acid Sequence; D000803:Angiotensin I / Q000737:chemistry; D000804:Angiotensin II / Q000737:chemistry; D000808:Angiotensinogen / Q000737:chemistry; D006801:Humans; D008969:Molecular Sequence Data; D008970:Molecular Weight; D007703:Peptidyl-Dipeptidase A / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D013363:Submandibular Gland / Q000201:enzymology*; D013379:Substrate Specificity
D000808:Angiotensinogen; D000804:Angiotensin II; D000803:Angiotensin I; D007703:Peptidyl-Dipeptidase A