Biotransformation, by Enterobacter agglomerans, of pyrimidine acyclonucleosides to acyclonucleotides.
The ability of Enterobacter agglomerans to transform naturally occurring nucleosides into nucleotides has been utilized to transform newly synthesized pyrimidine acyclonucleosides into the corresponding acyclonucleotides. Unselected bacteria were used as the source of nucleoside phosphotransferase, the phosphate group donor being 4-nitrophenyl phosphate in the presence of Zn2+ ions. Optimal reaction conditions are outlined.
Rutkowski
M
M
Department of General Chemistry, Institute of Basic Sciences, Military Medical Academy, Lodz, Poland.
Korczak
E
E
D004754:Enterobacter / Q000201:enzymology*; D009596:Nitrophenols / Q000378:metabolism; D009705:Nucleosides / Q000378:metabolism*; D009711:Nucleotides / Q000378:metabolism*; D009943:Organophosphorus Compounds / Q000378:metabolism; D010770:Phosphotransferases / Q000378:metabolism; D015032:Zinc / Q000494:pharmacology
D009596:Nitrophenols; D009705:Nucleosides; D009711:Nucleotides; D009943:Organophosphorus Compounds; C008644:nitrophenylphosphate; D010770:Phosphotransferases; C021892:nucleoside phosphotransferase; D015032:Zinc