Cell-mediated cytolysis of lymphoblastoid cells expressing Marek's disease virus-specific phosphorylated polypeptides.
Cell-mediated immune responses against Marek's disease virus (MDV)-antigens were examined using reticuloendotheliosis virus (REV)-transformed lymphoblastoid cell line CU91 and three cell lines derived from CU91. CU210 was established by establishing a latent MDV infection in CU91. Transfection of CU210 with pNL1, a selectable plasmid or with pNL1 and the cloned BamHI A fragment of MDV DNA resulted in the establishment of CU212 and CU211, respectively. CU211 expressed a MDV-specific phosphorylated polypeptide, while CU210 and CU212 were negative for MDV antigens. Only CU211 was lysed by MDV-specific effector cells. All cell lines were lysed by syngeneic REV-specific effector cells, although high levels of expression of the phosphorylated protein reduced the level of REV-specific lysis.
Pratt
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WD
Dept. of Avian and Aquatic Animal Medicine, Cornell University, Ithaca, NY 14853.
Morgan
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Schat
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D000818:Animals; D000956:Antigens, Viral / Q000235:genetics / Q000276:immunology*; D002461:Cell Line, Transformed; D002645:Chickens; D015967:Gene Expression Regulation, Viral; D008381:Herpesvirus 2, Gallid / Q000235:genetics / Q000276:immunology*; D007111:Immunity, Cellular; D010750:Phosphoproteins / Q000235:genetics / Q000276:immunology*; D014162:Transfection
D000956:Antigens, Viral; D010750:Phosphoproteins