New hepatocellular carcinoma cell line SUHC-1 established from a patient with hepatitis C virus RNA in serum.
A new human hepatocellular carcinoma cell (HCC) line, designated SUHC-1, was derived from a Japanese patient with hepatocellular carcinoma having antibody to hepatitis C virus (HCV) and HCV-RNA in his serum, and established in tissue culture. This cell line exhibited typical epithelial cell morphology in culture as observed by phase-contrast and electron microscopy. The SUHC-1 cells produced albumin and alpha 2-macroglobulin. Chromosomal analysis showed several rearrangements at short and long arms of chromosome 1, 17 and 20 (1p-, 1q-, i(1q), i(17q) and 20q+) with a modal number of 91. HCV-RNA was not detected in the supernatant of SUHC-1 cells by nested polymerase chain reaction assay or in the SUHC-1 cells by the in situ hybridization method. We concluded that complete HCV does not exist in the SUHC-1 cell line. The SUHC-1 cell line is the first line of HCC to have been derived from a patient with persistent HCV infection, and may provide a suitable model for studies of hepatocarcinogenesis related to HCV.
Yoshizawa
K
K
Second Department of Internal Medicine, Shinshu University School of Medicine, Matsumoto.
Kiyosawa
K
K
Usuda
S
S
Yabu
K
K
Nakatsuji
Y
Y
Yamada
S
S
Furuta
K
K
Tanaka
E
E
Sodeyama
T
T
Furuta
S
S
D000368:Aged; D000369:Aged, 80 and over; D001483:Base Sequence; D014408:Biomarkers, Tumor / Q000032:analysis; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000473:pathology* / Q000648:ultrastructure; D002455:Cell Division; D002878:Chromosomes, Human, Pair 1; D002886:Chromosomes, Human, Pair 17; D002890:Chromosomes, Human, Pair 20; D046508:Culture Techniques / Q000379:methods; D015321:Gene Rearrangement; D016174:Hepacivirus / Q000235:genetics / Q000302:isolation & purification* / Q000648:ultrastructure; D006801:Humans; D017403:In Situ Hybridization; D007621:Karyotyping; D007700:Kinetics; D008113:Liver Neoplasms / Q000235:genetics / Q000473:pathology* / Q000648:ultrastructure; D008297:Male; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D016376:Oligonucleotides, Antisense; D016133:Polymerase Chain Reaction / Q000379:methods; D012367:RNA, Viral / Q000097:blood* / Q000302:isolation & purification; D014407:Tumor Cells, Cultured
D014408:Biomarkers, Tumor; D009838:Oligodeoxyribonucleotides; D016376:Oligonucleotides, Antisense; D012367:RNA, Viral