Glycoprotein 60 of equine herpesvirus type 1 is a homologue of herpes simplex virus glycoprotein D and plays a major role in penetration of cells.
Monoclonal antibodies (MAbs) specific for equine herpesvirus type 1 (EHV-1) glycoprotein 60 (gp60) and gp 17/18 (F3132 and 5H6 respectively) were found to react with the same protein, which was identified as a homologue of herpes simplex virus type 1 gD. MAb F3132 strongly neutralized virus infectivity and inhibited the penetration of the virus into the cell. The effects on penetration were shared with three other MAbs against this protein (P68, F3116 and F3129), but no effect on virus penetration was found with any other anti-EHV-1 MAb tested. The level of glycosylation of gp60 was analysed using glycanase enzymes and glycosylation inhibitors, and consisted of mainly N-linked carbohydrate. The M(r) of non-N-glycosylated gp60 was 50K.
Whittaker
G R
GR
Department of Microbiology, University of Leeds, U.K.
Taylor
L A
LA
Elton
D M
DM
Giles
L E
LE
Bonass
W A
WA
Halliburton
I W
IW
Killington
R A
RA
Meredith
D M
DM
D000818:Animals; D000911:Antibodies, Monoclonal; D002241:Carbohydrates / Q000032:analysis; D006031:Glycosylation; D006566:Herpesviridae Infections / Q000378:metabolism; D004861:Herpesvirus 1, Equid / Q000235:genetics* / Q000472:pathogenicity; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D012689:Sequence Homology, Nucleic Acid; D018139:Simplexvirus / Q000235:genetics*; D014759:Viral Envelope Proteins / Q000235:genetics* / Q000276:immunology; D014774:Virulence; D014779:Virus Replication / Q000235:genetics*
D000911:Antibodies, Monoclonal; D002241:Carbohydrates; D014759:Viral Envelope Proteins; C488988:glycoprotein D, Equid herpesvirus 1; C075737:glycoprotein gp60, Equid herpesvirus 1