Inhibition of bovine papillomavirus plasmid DNA replication by adeno-associated virus.
The helper-dependent human parvovirus adeno-associated virus type 2 (AAV) inhibits both the oncogenic transforming abilities and the DNA replication of its helper viruses, adenovirus (Ad), and herpes simplex virus (HSV). As AAV-2 also inhibits the transforming ability of bovine papillomavirus type 1 (BPV), AAV-2 was assayed for its ability to inhibit BPV plasmid DNA replication. Here we find that the AAV-2 Rep78 gene is able to trans-inhibit BPV plasmid DNA replication and that the AAV-2 terminal repeats (TR) are also cis-required for the full inhibitory effect of AAV-2. When both the AAV-2 Rep78 open reading frame and TRs are present the inhibition of BPV plasmid DNA replication is very strong.
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Department of Obstetrics and Gynecology, University of Arkansas for Medical Sciences, Little Rock 72205.
D000818:Animals; D052688:Bovine papillomavirus 1 / Q000254:growth & development* / Q000472:pathogenicity; D004261:DNA Replication; D000229:Dependovirus / Q000235:genetics*; D015967:Gene Expression Regulation, Viral; D006378:Helper Viruses / Q000378:metabolism; D051379:Mice; D010957:Plasmids / Q000235:genetics