Protective effects of anti-glycoprotein D monoclonal antibodies in murine herpetic keratitis.
The protective effects of passive immunization with two kinds of anti-glycoprotein D (anti-gD) monoclonal antibodies, having different antiviral activities, were investigated in murine herpetic keratitis. One monoclonal antibody, designated M1, had high virus-neutralizing antibody titers, along with undetectable levels of complement-dependent cytolysis (CDC) and antibody-dependent cellular cytotoxicity (ADCC); the other, designated M12, exhibited extremely low titers of virus-neutralization with high level of CDC and ADCC. When systemically administered 24 hours prior to virus inoculation to the cornea, both M1 and M12 almost completely prevented the development of stromal keratitis. The protective efficacy of both was observed to be dose-dependent. Pepsin-treated M1 retained its efficacy in suppressing stromal keratitis, whereas pepsin-treated M12 did not. When the administration of M1 and M12 were delayed, both provided significant (but less complete) protection, up to 24 hours after virus inoculation. These results suggest that both virus neutralization and CDC/ADCC play an important role in preventing virus growth in the corneal stroma during the early stage of corneal infection.
Inoue
Y
Y
Department of Ophthalmology, Osaka University Medical School, Japan.
Ohashi
Y
Y
Watanabe
H
H
Manabe
R
R
D000818:Animals; D000911:Antibodies, Monoclonal / Q000008:administration & dosage* / Q000276:immunology; D000920:Antibody-Dependent Cell Cytotoxicity / Q000276:immunology; D003165:Complement System Proteins / Q000276:immunology; D005260:Female; D007116:Immunization, Passive; D007274:Injections, Intraperitoneal; D016849:Keratitis, Herpetic / Q000276:immunology / Q000517:prevention & control*; D051379:Mice; D008807:Mice, Inbred BALB C; D009500:Neutralization Tests; D018139:Simplexvirus / Q000276:immunology*; D014709:Vero Cells; D014759:Viral Envelope Proteins / Q000008:administration & dosage*
D000911:Antibodies, Monoclonal; D014759:Viral Envelope Proteins; C039447:glycoprotein D, Human herpesvirus 1; D003165:Complement System Proteins