Monoclonal antibody to human plasma gelsolin.
Human plasma gelsolin was purified by column chromatography. The method yielded a protein of high purity and activity. Using this protein, we produced monoclonal antibody (Mab H6B11) against human plasma gelsolin by somatic cell fusion. This monoclonal antibody reacted in a dose-dependent manner with gelsolin derived from human plasma and platelets and neutralized depolymerizing activity to F-actin. It differed from the commercially available substance (Mab G4896; Sigma) in that the time required for the reaction between the antigen and antibody in the enzyme-linked immunosorbent assay could be shortened by one-third. The antibody was judged to be useful in assays for elucidating the physiological role of plasma gelsolin.
Hiyoshi
M
M
Department of Laboratory Medicine, Osaka City University Medical School.
Terano
Y
Y
Tatsumi
N
N
Okuda
K
K
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000302:isolation & purification / Q000378:metabolism; D002135:Calcium-Binding Proteins / Q000097:blood / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification*; D002460:Cell Line; D004797:Enzyme-Linked Immunosorbent Assay; D018260:Gelsolin; D006801:Humans; D006825:Hybridomas; D051379:Mice; D008807:Mice, Inbred BALB C; D008840:Microfilament Proteins / Q000097:blood / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification*
D000911:Antibodies, Monoclonal; D002135:Calcium-Binding Proteins; D018260:Gelsolin; D008840:Microfilament Proteins