[The expression of plasmalemma transcobalamin-II receptors on human blood lymphocytes stimulated by mitogens].
The influence of mitogens on the expression of surface membrane TC-II receptors of human blood lymphocytes and internalization of (TS-II+57Co-CNCbl) complex into cytoplasm were investigated. Mature lymphocytes have a very small number of surface receptors to plasma TC-II but their expression is increased significantly by PHA or Con-A stimulation. CBl transport to cytoplasma is activated in definite sequence by two different mechanisms. Stimulated cells take free CBl without participation of TC-II in early hours of mitogen action (12-42 hrs) before maximal 3H-thymidine incorporation into DNA. On day 3 of cultivation, specific mechanism of CBl transport triggers and the number of lymphoblast receptors is increased manifold. Radioactive CBl enters cytoplasma due to interaction of TC-II-CN [57Co] CBl of the medium with surface membrane receptor of the cells. Thus, the definition of TC-II receptors as an important functional parameter may serve a marker of proliferating cells.
Oreshkin
A E
AE
Gudkova
M V
MV
Miasishcheva
N V
NV
D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D002478:Cells, Cultured / Q000187:drug effects / Q000378:metabolism; D003037:Cobalt Radioisotopes; D003208:Concanavalin A / Q000494:pharmacology; D006801:Humans; D008213:Lymphocyte Activation / Q000187:drug effects*; D008214:Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D008934:Mitogens / Q000494:pharmacology*; D010835:Phytohemagglutinins / Q000494:pharmacology; D011956:Receptors, Cell Surface / Q000187:drug effects* / Q000378:metabolism; D014155:Transcobalamins / Q000187:drug effects* / Q000378:metabolism; D014316:Tritium; D014805:Vitamin B 12 / Q000097:blood
D003037:Cobalt Radioisotopes; D008934:Mitogens; D010835:Phytohemagglutinins; D011956:Receptors, Cell Surface; D014155:Transcobalamins; C028176:transcobalamin receptor; D014316:Tritium; D003208:Concanavalin A; D014805:Vitamin B 12
Expressiia retseptorov transkobalamina-II plazmalemmy limfotsitov krovi cheloveka, stimulirovannykh mitogenami.