title: Transformation of cauliflower (Brassica oleracea L. var. botrytis)--an experimental survey.
abstract: The paper compares different approaches for the genetic transformation of cauliflower (Agrobacterium-mediated, PEG-mediated and/or electroporation). Transient expression of the neomycin phosphotransferase II (NPTII) gene could be detected after direct gene transfer. Stable transformation was achieved using both Agrobacterium-mediated and direct gene transfer. Expression as well as incorporation of the NPTII sequence could be demonstrated.
authors.affiliation: Dept. of Genetics, Martin Luther University, Halle/S., Germany.
authors.forename: K
authors.initials: K
authors.lastname: Eimert
authors.forename: F
authors.initials: F
authors.lastname: Siegemund
mesh_terms: D001937:Brassica / Q000235:genetics*; D019868:Kanamycin Kinase; D010770:Phosphotransferases / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D010957:Plasmids; D011523:Protoplasts / Q000201:enzymology; D012231:Rhizobium / Q000235:genetics*; D014162:Transfection; D014170:Transformation, Genetic
chemical_list: D010770:Phosphotransferases; D019868:Kanamycin Kinase