Suppression mediated in vitro by Marek's disease virus-transformed T-lymphoblastoid cell lines: effect on lymphoproliferation.
Marek's disease virus (MDV)-transformed lymphoblastoid cells (MDCC-MSB1, -PA9 and -RP1) added to chicken splenic lymphocytes after treatment with mitomycin, suppress the lymphoproliferative response to T-cell mitogens (concanavalin A or phytohemagglutinin) by 40-70%. This suppressive activity was observed in syngeneic as well as in allogeneic combinations of cell lines and responder lymphocytes. The suppressive effect disappeared when the addition of MD-transformed cell lines to the responder cultures was delayed for 24 h. Treatment with glutaraldehyde, instead of mitomycin, greatly weakened the suppressive activity of the MD lymphoblastoid cells. A reduction of interleukin 2 (IL-2)-like activity produced by responder lymphocytes was observed after mixing with mitomycin-treated lymphoblastoid cells, but also, although slightly less, with the same glutaraldehyde-treated cells. Nevertheless no membrane fluorescence was observed, using INN-CH16 monoclonal antibody on MDV-induced lymphoblastoid cell lines to check up on the presence of IL-2 receptor-like structure. All the three lines exhibited a CD4+, CD8- phenotype.
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Unité de Virologie Aviaire, Institut National de la Recherche Agronomique, CR de Tours-Nouzilly, Monnaie, France.
D000818:Animals; D000911:Antibodies, Monoclonal; D002460:Cell Line; D002472:Cell Transformation, Viral / Q000276:immunology*; D002645:Chickens; D005976:Glutaral / Q000494:pharmacology; D008381:Herpesvirus 2, Gallid / Q000276:immunology*; D016130:Immunophenotyping; D007165:Immunosuppression Therapy; D007213:Indomethacin / Q000494:pharmacology; D007376:Interleukin-2 / Q000096:biosynthesis; D008213:Lymphocyte Activation / Q000276:immunology*; D008297:Male; D008380:Marek Disease / Q000276:immunology / Q000473:pathology; D008934:Mitogens; D008937:Mitomycins / Q000494:pharmacology; D015375:Receptors, Interleukin-2 / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology* / Q000382:microbiology
D000911:Antibodies, Monoclonal; D007376:Interleukin-2; D008934:Mitogens; D008937:Mitomycins; D015375:Receptors, Interleukin-2; D005976:Glutaral; D007213:Indomethacin