title: Detection of human papillomavirus genome and analysis of expression of c-myc and Ha-ras oncogenes in invasive cervical carcinomas.
abstract: Invasive carcinomas of the uterine cervix of 38 patients were examined for the presence of human papillomavirus (HPV) genomes and for the state of the c-myc and Ha-ras oncogenes. A combination of Southern blot hybridization and polymerase chain reaction revealed the presence of the genome of HPV type 16 in 17 tumors (45%), that of HPV type 18 in 3 tumors (8%), and that of unknown types in 16 others (42%), while no viral DNA sequences were detected in 2 tumors. Of the 38 tumors, c-myc amplification was found in only 1 tumor, while there was no Ha-ras amplification. Overexpression of the c-myc gene was observed in 15 (44%) of the 34 tumors analyzed, while there was no overexpression of Ha-ras. Of the 23 squamous cell carcinomas analyzed, relapse-free rates at 24 months were 55% in tumors with c-myc overexpression and 100% in case of tumors with no c-myc overexpression, respectively. The results suggest the possibility that activation of the c-myc oncogene is involved in tumor progression.
authors.affiliation: Department of Obstetrics and Gynecology, Saga Medical School, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Iwasaka
authors.forename: M
authors.initials: M
authors.lastname: Yokoyama
authors.forename: M
authors.initials: M
authors.lastname: Oh-uchida
authors.forename: N
authors.initials: N
authors.lastname: Matsuo
authors.forename: K
authors.initials: K
authors.lastname: Hara
authors.forename: K
authors.initials: K
authors.lastname: Fukuyama
authors.forename: T
authors.initials: T
authors.lastname: Hachisuga
authors.forename: K
authors.initials: K
authors.lastname: Fukuda
authors.forename: H
authors.initials: H
authors.lastname: Sugimori
mesh_terms: D001483:Base Sequence; D015344:DNA Probes, HPV; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D005814:Genes, Viral; D016259:Genes, myc; D011905:Genes, ras; D006801:Humans; D008969:Molecular Sequence Data; D027383:Papillomaviridae / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000032:analysis; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000473:pathology
chemical_list: D015344:DNA Probes, HPV; D004279:DNA, Viral; D012367:RNA, Viral