Insertion of SMRV-H viral DNA at the c-myc gene locus of a BL cell line and presence in established cell lines.
Mutation of the c-myc gene locus has occurred during in vitro culture of the Burkitt lymphoma cell line Namalwa. Gene cloning and sequencing of this c-myc gene locus has revealed the insertion of incomplete copies of the Squirrel Monkey Retrovirus-Human (SMRV-H) proviral genome. Insertions of the SMRV-H genome were seen in all Namalwa cell lines tested, with multiple insertions being seen in some sublines. Viral particles with the morphology of type-D retrovirus, budding from these cells, were seen by transmission EM. New insertions of the genome were produced spontaneously in these cells. The origins of the type-D retrovirus and its implications for workers in the field are discussed.
Middleton
P G
PG
LRF Lab, Medical School, Newcastle upon Tyne, UK.
Miller
S
S
Ross
J A
JA
Steel
C M
CM
Guy
K
K
D001483:Base Sequence; D002051:Burkitt Lymphoma / Q000235:genetics / Q000382:microbiology*; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004251:DNA Transposable Elements; D004279:DNA, Viral / Q000032:analysis*; D016259:Genes, myc; D006801:Humans; D008242:Lysogeny; D008969:Molecular Sequence Data; D015301:Retroviruses, Simian / Q000235:genetics*; D014407:Tumor Cells, Cultured
D004251:DNA Transposable Elements; D004279:DNA, Viral