Limited potential for mosquito transmission of a live, attenuated chikungunya virus vaccine.
Studies were conducted to determine the potential for transmission of a live, attenuated chikungunya (CHIK) virus vaccine by orally exposed or virus-inoculated mosquitoes. The vaccine (CHIK 181/clone 25) replicated in and was transmitted by female Aedes albopictus and Ae. aegypti after intrathoracic inoculation. Mosquitoes also became infected with the vaccine after ingesting virus from either a blood-soaked cotton pledget or a viremic monkey. However, because of the low viremias produced in inoculated humans, it is unlikely that mosquitoes would become infected by feeding on a person inoculated with the live, attenuated CHIK vaccine. Although the vaccine was transmitted by mosquitoes after intrathoracic inoculation, there was no evidence of reversion to a virulent phenotype.
Turell
M J
MJ
Department of Arboviral Entomology, U.S. Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, Maryland.
Malinoski
F J
FJ
D000330:Aedes / Q000382:microbiology*; D000818:Animals; D000833:Animals, Suckling; D002646:Chikungunya virus / Q000276:immunology* / Q000472:pathogenicity / Q000502:physiology; D005260:Female; D007303:Insect Vectors / Q000382:microbiology*; D008253:Macaca mulatta; D051379:Mice; D014036:Togaviridae Infections / Q000635:transmission*; D014613:Vaccines, Attenuated / Q000009:adverse effects; D014765:Viral Vaccines / Q000009:adverse effects*; D014774:Virulence; D014779:Virus Replication
D014613:Vaccines, Attenuated; D014765:Viral Vaccines