title: [Morphology and delta-endotoxin proteins of Bacillus thuringiensis from soils and their toxicities to insects].
abstract: 94 strains of Bacillus thuringiensis were isolated from soils in southwest and northwest of China. The morphology of cells, spores and parasporal crystals of these strains was investigated under transmission and scanning electro-microscope. Proteins of delta-endotoxins from all strains were analysed by rapid SDS-PAGE. 9 species of insects in Lepidoptera, Coleoptera and Diptera were tested for assay of delta-endotoxins. Some kinds of parasporal crystals were quite different in form and in composition of protein from those reported before. Most of strains were nontoxic to all of 9 species used in bio-assay. Some strains were very effective in species of Coleoptera or Noctuidae.
authors.affiliation: Wuhan Institute of Virology, Academia Sinica.
authors.forename: R
authors.initials: R
authors.lastname: Li
authors.forename: S
authors.initials: S
authors.lastname: Dai
authors.forename: X
authors.initials: X
authors.lastname: Li
authors.forename: Y
authors.initials: Y
authors.lastname: Zhao
authors.forename: C
authors.initials: C
authors.lastname: Sun
mesh_terms: D000818:Animals; D001413:Bacillus thuringiensis / Q000737:chemistry / Q000302:isolation & purification / Q000648:ultrastructure*; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D001517:Coleoptera; D004175:Diptera; D004731:Endotoxins / Q000302:isolation & purification; D006460:Hemolysin Proteins; D007306:Insecticides; D007915:Lepidoptera; D010572:Pest Control, Biological; D012988:Soil Microbiology
chemical_list: D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004731:Endotoxins; D006460:Hemolysin Proteins; D007306:Insecticides; C030956:insecticidal crystal protein, Bacillus Thuringiensis