[Taxonometric analysis of DNA repeating elements].
The method of analysis of low molecular weight fragments of high copied repeats of DNA hydrolysed by restriction nuclease is presented. The P labeled fragments (by means of DNA polymerase I) are electrophoresed in nondenaturing PAAG and radio-autographed. The specific band patterns are observed in region between approximately 20 and 300 bp. When studying some lizard and fish species DNA's it was shown that the patterns observed have species and genus specificity but not individual. The approach supposed can be applied to investigation of interspecies relationships, some evolutionary problems and to the studying of questions concerning the role of DNA repeats in evolution. The method is simple and comparatively cheap and may be called as "taxonomic DNA fingerprint" or "DNA taxonoprint" method.
Fedorov
A N
AN
Grechko
V V
VV
Slobodianiuk
S Ia
SIa
Fedorova
L V
LV
Timokhina
G I
GI
D000818:Animals; D001345:Autoradiography; D004247:DNA / Q000737:chemistry*; D016172:DNA Fingerprinting; D004591:Electrophoresis, Polyacrylamide Gel; D005399:Fishes / Q000097:blood; D008116:Lizards / Q000097:blood; D012091:Repetitive Sequences, Nucleic Acid; D009626:Terminology as Topic
D004247:DNA
Taksonomicheskiĭ analiz povtoriaiushchikhsia élementov DNK.