Identification of human and bovine rotavirus serotypes by polymerase chain reaction.
The use of the polymerase chain reaction (PCR) for identifying serotypes of human and bovine rotaviruses was examined. In the identification of 115 human rotavirus samples in stools, results with PCR showed excellent agreement with results of serotyping by an enzyme-linked immunosorbent assay (ELISA) using serotype-specific monoclonal antibodies. Furthermore, the PCR showed a much higher sensitivity (93%) than the ELISA test (82.6%). The PCR method could also be applied for identifying the serotype of bovine rotaviruses.
Taniguchi
K
K
Department of Hygiene, Sapporo Medical College, Japan.
Wakasugi
F
F
Pongsuwanna
Y
Y
Urasawa
T
T
Ukae
S
S
Chiba
S
S
Urasawa
S
S
D000818:Animals; D001483:Base Sequence; D002417:Cattle; D004247:DNA; D004797:Enzyme-Linked Immunosorbent Assay / Q000592:standards; D005069:Evaluation Studies as Topic; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012401:Rotavirus / Q000145:classification* / Q000235:genetics; D012400:Rotavirus Infections / Q000453:epidemiology* / Q000382:microbiology; D012680:Sensitivity and Specificity; D012703:Serotyping / Q000379:methods* / Q000592:standards
D004247:DNA