The nature of extra-chromosomal maintenance of transforming plasmids in the filamentous basidiomycete Phanerochaete chrysosporium.
The nature of extra-chromosomal maintenance of the transforming plasmid p12-6 in Phanerochaete chrysosporium was studied. Our results indicate that the plasmid is maintained in the fungal transformants extra-chromosomally as part of a larger endogenous plasmid (designated pME) of P. chrysosporium. Using the total DNA of p12-6 fungal transformants, p12-6, as well as a larger plasmid, p511, were recovered in recA- E. coli strains while only p12-6 was recovered in recA+ E. coli strains. The results also showed that the cytosine methylation system has no apparent effect on the strain-dependent recovery of p12-6 and p511 in E. coli from the total DNA of fungal transformants.
Randall
T A
TA
Department of Microbiology, Michigan State University, East Lansing 48824-1101.
Reddy
C A
CA
D001487:Basidiomycota / Q000235:genetics*; D003596:Cytosine / Q000378:metabolism; D004262:DNA Restriction Enzymes / Q000502:physiology; D004352:Drug Resistance, Microbial / Q000235:genetics; D005822:Genetic Vectors; D005839:Gentamicins / Q000494:pharmacology; D008745:Methylation; D010957:Plasmids / Q000502:physiology*; D014170:Transformation, Genetic / Q000502:physiology*
D005839:Gentamicins; D003596:Cytosine; C010680:antibiotic G 418; D004262:DNA Restriction Enzymes