[Transposition of Tn917 in Bacillus pumilus].
Transposition Tn917 was introduced into Bacillus pumilus 289 by protoplast transformation with plasmid pTV32. The temperature-sensitive replication property of pTV32 was maintained in B. pumilus. Tn917 was transposed efficiently in B. pumilus with 4.8 x 10(-4) transposition rate. The yield of auxotrophs was about 0.65% in all insertional mutants. It indicated a prospects for the use of Tn917 as a tool for insertional mutagenesis and genetic manipulation in B. pumilus.
Geng
Y
Y
Department of Biology, Nankai University, Tianjin.
Jiang
R
R
D001407:Bacillus / Q000235:genetics*; D004251:DNA Transposable Elements; D010957:Plasmids
D004251:DNA Transposable Elements