Inhibition of phosphoinositide turnover by praziquantel in Schistosoma mansoni.
The effect of praziquantel on phosphoinositide turnover was examined in Schistosoma mansoni to determine if this anthelminthic modulates signal transduction pathways in parasites. Adult worms were radiolabeled with [3H]myoinositol for 24 hr and total inositol phosphate levels determined in the presence of praziquantel. Praziquantel inhibited inositol phosphate turnover when activated with NaF plus AlCl3 or with the nonhydrolyzable guanine nucleotide-binding protein analogue GTP gamma S. Furthermore, praziquantel decreased basal turnover of inositol phosphates. Inhibition was seen in both male and female worms as well as in schistosomula. These data indicate that inhibition of phosphoinositide turnover may contribute to the effect of praziquantel on parasite survival within the definitive host.
Wiest
P M
PM
Division of Geographic Medicine, Miriam Hospital, Providence, Rhode Island.
Li
Y
Y
Olds
G R
GR
Bowen
W D
WD
D000818:Animals; D004305:Dose-Response Relationship, Drug; D005260:Female; D008297:Male; D010716:Phosphatidylinositols / Q000378:metabolism*; D011223:Praziquantel / Q000494:pharmacology*; D012550:Schistosoma mansoni / Q000187:drug effects* / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects*
D010716:Phosphatidylinositols; D011223:Praziquantel