Increased platelet sodium-proton exchange rates in insulin-dependent (type 1) diabetic patients with nephropathy and hypertension.
In order to assess the potential role of the plasma membrane sodium-proton (Na+/H+) exchanger in the pathogenesis of diabetic nephropathy, we investigated 32 insulin dependent (type 1) diabetic patients and 21 control subjects. We tested the Na+/H+ exchange as the rate of amiloride sensitive and sodium dependent volume gain of platelets suspended in sodium propionate. Patients with diabetic nephropathy had significantly increased rates of Na+/H+ exchange (0.31 +/- 0.06 s-1 x 10(-2)) when compared to those without nephropathy (0.24 +/- 0.07, p less than 0.05) or to a control group (0.23 +/- 05, p less than 0.05). Nine patients who were classified as hypertensive had a highly significant increase in the Na+/H+ exchange rates when compared to 23 non-hypertensive diabetic patients: 0.33 +/- 0.04 versus 0.24 +/- 0.06 (p less than 0.001). There was no significant correlation between the Na+/H+ exchange rates and age, diabetes duration, glycated hemoglobin or fructosamine levels on the day of the test. In summary, the data presented here demonstrate an increase in the Na+/H+ exchange rate in insulin-dependent diabetic patients with nephropathy and hypertension.
Barbe
P
P
Department of Endocrinology, CHU Purpan, Toulouse, France.
Salles
J P
JP
Barthe
P
P
Louvet
J P
JP
Chap
H
H
D000293:Adolescent; D000328:Adult; D000584:Amiloride / Q000494:pharmacology; D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism*; D002352:Carrier Proteins / Q000097:blood*; D003922:Diabetes Mellitus, Type 1 / Q000097:blood* / Q000150:complications; D003928:Diabetic Nephropathies / Q000097:blood* / Q000209:etiology; D005260:Female; D019270:Fructosamine; D006442:Glycated Hemoglobin / Q000032:analysis; D006595:Hexosamines / Q000097:blood; D006801:Humans; D006973:Hypertension / Q000097:blood* / Q000150:complications; D006977:Hypertension, Renal / Q000097:blood / Q000209:etiology; D008297:Male; D008875:Middle Aged; D011507:Proteinuria / Q000097:blood / Q000209:etiology; D012964:Sodium / Q000097:blood*; D017923:Sodium-Hydrogen Exchangers
D000584:Amiloride; D002352:Carrier Proteins; D019270:Fructosamine; D006442:Glycated Hemoglobin; D006595:Hexosamines; D012964:Sodium; D017923:Sodium-Hydrogen Exchangers