[Interaction of proflavin with deoxytetraribonucleoside triphosphate 5'-d(ApGpCpT): thermodynamic analysis from (1)H NMR data].
Temperature relationships of chemical shifts of protons of proflavin mixed with deoxytetraribonucleoside triphosphate 5'-d(ApGpCpT) in water solution were investigated on impulse NMR spectrometer (500 MHz). Procedure is suggested for calculating values of mole fractions of various associates in solution as a function against temperature. Free energies of Gibbs, entalpy and entropy were determined in the reactions of complex formation 1:1, 1:2, 2:1 of proflavin with tetranucleotide. The results point to a significant role of hydrophobic interactions during the formation of dye--tetramere duplex complexes.
Veselkov
A N
AN
Sevastopol Instrument-Making Institute, Burkback College, London University, Great Britain.
Davis
D
D
Dymant
L N
LN
Parkes
H
H
Shipp
D
D
D009682:Magnetic Resonance Spectroscopy; D009843:Oligoribonucleotides / Q000737:chemistry*; D011370:Proflavine / Q000737:chemistry*; D011522:Protons; D013816:Thermodynamics
D009843:Oligoribonucleotides; D011522:Protons; D011370:Proflavine
Vzaimodeĭstvie proflavina s dezoksitetraribonukleozidtrifosfatom 5'-d(ApGpCpT): termodinamicheskiĭ analiz po dannym (1)H IaMR.