title: Recombinant polypeptide from the gp48 region of the bovine viral diarrhea virus (BVDV) detects serum antibodies in vaccinated and infected cattle.
abstract: To characterize the immune response of cattle to bovine viral diarrhea virus (BVDV) glycoprotein gp48, we have produced a large amount of recombinant glutathione-s-transferase-gp48 (GST-gp48) fusion protein in Escherichia coli. Antibodies to gp48 were present in cattle vaccinated with killed or modified-live virus vaccination, or following natural infection. These results were in agreement with results of serum neutralization (SN) test which detected gp53 of BVDV.
authors.affiliation: USDA, ARS, U.S. Meat Animal Research Center, Clay Center, NE 68933.
authors.forename: J
authors.initials: J
authors.lastname: Kwang
authors.forename: E T
authors.initials: ET
authors.lastname: Littledike
authors.forename: R O
authors.initials: RO
authors.lastname: Donis
authors.forename: E J
authors.initials: EJ
authors.lastname: Dubovi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D001483:Base Sequence; D015153:Blotting, Western; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000276:immunology*; D002417:Cattle; D001908:Diarrhea Viruses, Bovine Viral / Q000276:immunology*; D015967:Gene Expression Regulation, Viral; D005822:Genetic Vectors; D005982:Glutathione Transferase / Q000737:chemistry; D006023:Glycoproteins / Q000737:chemistry / Q000276:immunology*; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D011993:Recombinant Fusion Proteins / Q000737:chemistry; D014611:Vaccination / Q000662:veterinary; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000276:immunology*; D014765:Viral Vaccines / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D006023:Glycoproteins; D011993:Recombinant Fusion Proteins; D014759:Viral Envelope Proteins; D014765:Viral Vaccines; C078531:glycoprotein 48, bovine viral diarrhea virus; D005982:Glutathione Transferase