Detection and analysis of human papillomavirus type 1 infection of skin warts from a dermatology clinic in Taiwan by southern hybridization.
Thirty cases of skin warts from the Dermatology Clinic at Tri-Service General Hospital were analyzed for the presence of human papillomavirus type 1 sequences by Southern blot hybridization. Thirteen of the 30 cases were HPV-1 positive. The prevalence was 43%. Episomal HPV-1 sequences were detected in 11 of 13 HPV-1 positive cases. There are two cases which probably contained integrated forms, one contained the higher molecular weight bands, and the other with 6 kb viral genome may be the result of rearrangement and deletion. Histological studies from HPV-1 positive specimens also indicated the typical features of HPV infection. Some cases with high copy number had a high frequency of inclusion bodies. Chi-square analysis showed that HPV-1 prevalence is not related to sex and different lesion locations, but the prevalence of HPV-1 in recurrent lesions is higher than that of initial lesions.
Sytwu
H K
HK
Department of Microbiology and Immunology, National Defense Medical Center, Taipei, Taiwan, R. O. C.
Sheu
W J
WJ
Shih
J M
JM
Chen
S L
SL
D000293:Adolescent; D000328:Adult; D000368:Aged; D015139:Blotting, Southern; D002648:Child; D002675:Child, Preschool; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification*; D015995:Prevalence; D012867:Skin / Q000382:microbiology; D014412:Tumor Virus Infections / Q000175:diagnosis* / Q000453:epidemiology / Q000473:pathology; D014860:Warts / Q000382:microbiology* / Q000473:pathology
D004279:DNA, Viral