title: [Indirect ultramicroElisa for the detection of total antibodies against cytomegaloviruses in human blood].
abstract: We have standardized an indirect ultramicro ELISA assay for detecting antibodies to human Cytomegalovirus (CMV) using human serum samples (UMELISA CMV). The optimal concentration of coating antigen (30 ug/ml), serum dilution (1:40) and anti-human conjugate working dilution (1:1500), were determined by a check board titration method. The UMELISA CMV was compared with the latex agglutination test for antibodies to CMV (Dupont de Nemours) and with an indirect immunofluorescent method. The results have showed the high coincidence, sensitivity and especificity of the proposed assay regarding the two methods compared with, and supporting its use either for a blood donors screening or in the serological diagnosis of this infection by paired serum samples.
authors.affiliation: Departmento de Virologia, Instituto de Medicina Tropical, Pedro Kouri Siboney, Ciudad Habana, Cuba.
authors.forename: J
authors.initials: J
authors.lastname: Laferte
authors.forename: M
authors.initials: M
authors.lastname: Marrero
authors.forename: M
authors.initials: M
authors.lastname: Alvarez
authors.forename: L
authors.initials: L
authors.lastname: Jomarron
authors.forename: S
authors.initials: S
authors.lastname: Garcia
authors.forename: S
authors.initials: S
authors.lastname: Vazquez
authors.forename: L
authors.initials: L
authors.lastname: Morier
authors.forename: M
authors.initials: M
authors.lastname: Ulacia
authors.forename: A
authors.initials: A
authors.lastname: Melchor
mesh_terms: D000914:Antibodies, Viral / Q000097:blood*; D001771:Blood Banks; D003587:Cytomegalovirus / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005455:Fluorescent Antibody Technique; D006801:Humans; D007841:Latex Fixation Tests; D011237:Predictive Value of Tests
chemical_list: D000914:Antibodies, Viral
vernacular_title: UltramicroELISA indirecto para la detección de anticuerpos totales a Citomegalovirus en suero humano.