Evaluation of three methods for the concentration of poliovirus from oysters.
Three methods for the concentration of poliovirus from oyster homogenates were compared. The adsorption-elution-precipitation method gave the lowest average virus recovery (24.1%), while the beef extract elution-acid precipitation method and the non-fat dry milk elution-acid precipitation methods gave recoveries of 47.2% and 39.6%, respectively. Although the overall recovery rates with these methods were lower than those reported in previous studies, recoveries of 40-47% obtained with the elution-precipitation methods used in the present study are considered to be above average in terms of recovery efficiency.
Bouchriti
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Departement d'Hygiene et Industrie des Denrees Alimentaires d'Origine Animale, Institut Agronomique et Veterinaire Hassan II, Rabat, Morocco.
Goyal
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SM
D000818:Animals; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D005069:Evaluation Studies as Topic; D010125:Ostreidae / Q000382:microbiology*; D017955:Poliovirus / Q000302:isolation & purification*; D014776:Virus Cultivation / Q000379:methods*