title: The pseudorabies virus homology of the herpes simplex virus UL21 gene product is a capsid protein which is involved in capsid maturation.
abstract: We mutagenized, mapped, and sequenced the pseudorabies virus (PRV) homology of gene UL21 of herpes simplex virus type 1. A polyclonal mouse antiserum against the protein encoded by the UL21 homolog was generated and used to monitor the expression and subcellular localization of the UL21-encoded protein. We found that the protein is identical to a previously detected PRV capsid protein. We analyzed viable PRV strains encoding mutant UL21 homologys, truncated by insertion of an oligonucleotide that contains stop codons in all reading frames. In two PRV mutants carrying the oligonucleotide at two sites within the gene, processing of newly replicated viral DNA was impaired. In addition, we show that one of the UL21 mutants has strongly reduced virulence for mice.
authors.affiliation: Division of Molecular Genetics, The Netherlands Cancer Institute, Amsterdam.
authors.forename: N
authors.initials: N
authors.lastname: de Wind
authors.forename: F
authors.initials: F
authors.lastname: Wagenaar
authors.forename: J
authors.initials: J
authors.lastname: Pol
authors.forename: T
authors.initials: T
authors.lastname: Kimman
authors.forename: A
authors.initials: A
authors.lastname: Berns
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002213:Capsid / Q000235:genetics*; D036022:Capsid Proteins; D002460:Cell Line; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification; D005814:Genes, Viral; D011558:Herpesvirus 1, Suid / Q000235:genetics* / Q000472:pathogenicity; D014645:Herpesvirus 3, Human / Q000235:genetics; D007668:Kidney; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D016366:Open Reading Frames; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000302:isolation & purification / Q000378:metabolism; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification; D016364:Reading Frames; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D018139:Simplexvirus / Q000235:genetics*; D013552:Swine; D014158:Transcription, Genetic; D014774:Virulence
chemical_list: D036022:Capsid Proteins; D004279:DNA, Viral; D012333:RNA, Messenger; D012367:RNA, Viral; C078030:UL21 protein, Pseudorabies virus