Glycolipids of a human glioma cell line bearing receptors for platelet-derived growth factor (PDGF).
Glycolipids of U-1242 MG were characterized because results of previous studies showed that exogenous gangliosides, especially GM3, inhibit PDGF-stimulated growth of this human glioma cell line. GM3 and GM2 are the major gangliosides; both separate as doublets with thin-layer chromatography. The major neutral glycolipid is glucocerebroside with nonhydroxy fatty acids, but paragloboside, ceramide dihexoside, globoside, and asialoGM2 (GA2) are also present. The coexistence in U-1242 MG of these gangliosides and the PDGF receptor, whose mitogenic signal is modulated by GM3 in these cells, suggests a possible functional relationship among them with respect to growth regulation.
Yates
A J
AJ
Department of Pathology, Ohio State University, Columbus 43210.
Agudelo
J D
JD
Sung
C C
CC
D002855:Chromatography, Thin Layer; D005732:Gangliosides / Q000032:analysis*; D005910:Glioma / Q000378:metabolism; D006017:Glycolipids / Q000032:analysis*; D006801:Humans; D010982:Platelet-Derived Growth Factor / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000032:analysis*; D017479:Receptors, Platelet-Derived Growth Factor; D014407:Tumor Cells, Cultured
D005732:Gangliosides; D006017:Glycolipids; D010982:Platelet-Derived Growth Factor; D011956:Receptors, Cell Surface; D017479:Receptors, Platelet-Derived Growth Factor