5'-upstream cis-elements and binding factor(s) potentially involved in light-regulated expression of a Brassica napus rbcS gene.
The 5'-upstream region of a Brassica napus rbcS gene contains sequence elements that resemble the cis-regulatory elements described for other species. In band shift competition assays using whole-cell extracts and sets of deletion fragments we could assign protein binding to a region located 110-130 base pairs upstream of the transcription start site. DNase I protection experiments showed that the binding region is non-contiguous and reveals symmetry. On the non-coding strand, the sequence contains 5'-CAC-3' and 5'-GTGG-3' elements which together resemble the contiguous G-box core motif, CACGTGG, of other rbcS genes. The DNA-protein interaction was more efficient with extracts from light-grown than with those from dark-grown seedlings, indicating the possible involvement of light-regulated factors(s).
Fiebig
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University of Bochum, Plant Cell Physiology and Molecular Biology, Federal Republic of Germany.
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D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D001937:Brassica / Q000235:genetics* / Q000528:radiation effects; D003001:Cloning, Molecular; D004247:DNA; D005786:Gene Expression Regulation; D008027:Light; D008969:Molecular Sequence Data; D005810:Multigene Family; D012045:Regulatory Sequences, Nucleic Acid; D015183:Restriction Mapping; D012273:Ribulose-Bisphosphate Carboxylase / Q000235:genetics*; D014157:Transcription Factors / Q000378:metabolism*; D014158:Transcription, Genetic
D014157:Transcription Factors; D004247:DNA; D012273:Ribulose-Bisphosphate Carboxylase