Biokinetics and biliary excretion of radiotocopherol administered orally to sheep.
Four adult sheep were given .4 microCi/kg of BW of D-alpha[5-Me-3H]tocopherol orally. Plasma alpha-tocopherol specific activities were measured serially during a 72-h period, after which the sheep were killed. The disposition kinetics were best described by the use of a two-compartment model. The radiotocopherol had a slow distribution phase followed by a relatively slow elimination phase. During the 72-h period of serial monitoring the peak plasma specific activities were observed between 32 to 48 h. At slaughter (72 h after dosing) tissue radioactivity distribution indicated a high rate of accumulation in some glandular organs such as the liver and adrenals. Identification of radioactive components excreted in bile, using HPLC or thin-layer chromatography, showed that unchanged radiotocopherol was present only at very low concentrations (less than 3% of the total recovered radioactivity).
Hidiroglou
M
M
Animal Research Centre, Agriculture Canada, Ottawa, Ontario.
Ivan
M
M
D000284:Administration, Oral; D000818:Animals; D001646:Bile / Q000737:chemistry / Q000378:metabolism*; D001682:Biological Availability; D004043:Dietary Fiber / Q000008:administration & dosage; D005766:Gastrointestinal Contents / Q000737:chemistry; D007408:Intestinal Absorption; D008297:Male; D006109:Poaceae; D012756:Sheep / Q000378:metabolism*; D012820:Silage; D014018:Tissue Distribution; D014810:Vitamin E / Q000008:administration & dosage / Q000032:analysis / Q000493:pharmacokinetics*; D003313:Zea mays
D004043:Dietary Fiber; D014810:Vitamin E