Active protection of mice against Salmonella typhi by immunization with strain-specific porins.
NIH mice were immunized with between 2.5 and 30 micrograms of two highly purified porins, 34 kDa and 36 kDa, isolated from the virulent strain Salmonella typhi 9,12, Vi:d. Of mice immunized with 10 micrograms of porins, 90% were protected against a challenge with up to 500 LD50 (50% lethal doses) of S. typhi 9,12,Vi:d and only 30% protection was observed in mice immunized with the same dose of porins but challenged with the heterologous strain Salmonella typhimurium. These results demonstrate the utility of porins for the induction of a protective status against S. typhi in mice.
Isibasi
A
A
Laboratorio de Inmmunoquímica, Instituto Mexicano del Seguro Social, México DF.
Ortiz-Navarrete
V
V
Paniagua
J
J
Pelayo
R
R
González
C R
CR
García
J A
JA
Kumate
J
J
D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology* / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid; D005260:Female; D051379:Mice; D018272:Porins; D012485:Salmonella typhi / Q000276:immunology*; D014435:Typhoid Fever / Q000276:immunology / Q000517:prevention & control*; D014611:Vaccination
D001425:Bacterial Outer Membrane Proteins; D018272:Porins