Structures of poly(dA-dT, ip5dU) containing various small amounts of the antiherpetic 5-isopropyl-2'-deoxyuridine.
Three different concentrations of the antiherpetic agent 5-isopropyl-2'-deoxyuridine (ip5dU) were introduced into the synthetic DNA poly(dA-dT) to analyze resulting copolymers by electron microscopy, UV absorption and CD spectroscopy. The poly(dA-dT, ip5dU) containing 1.3 and 4.3% ip5dU did not much differ from the parent poly(dA-dT) but poly (dA-dT, ip5dU) with 7.1% ip5dU behaved in an unusual way. Results are explained by the notion that if bulky isopropyls occur sufficiently close to each other then stable hairpins protruding from the double helix are formed, presumably to accommodate the ip5dU-s into the loops.
Sági
J
J
Central Research Insitute for Chemistry, Hungarian Academy of Sciences, Budapest.
Stokrová
J
J
Vorlícková
M
M
Spánová
A
A
Kypr
J
J
Ruff
E
E
Otvös
L
L
D000998:Antiviral Agents / Q000737:chemistry* / Q000627:therapeutic use; D002942:Circular Dichroism; D003857:Deoxyuridine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000627:therapeutic use; D006566:Herpesviridae Infections / Q000188:drug therapy; D008854:Microscopy, Electron; D009690:Nucleic Acid Conformation; D011067:Poly dA-dT / Q000737:chemistry*; D011089:Polydeoxyribonucleotides / Q000737:chemistry* / Q000627:therapeutic use; D013056:Spectrophotometry, Ultraviolet
D000998:Antiviral Agents; D011089:Polydeoxyribonucleotides; C074875:poly(deoxyadenine-thymine,5-isopropyl-2'-deoxyuridine); D011067:Poly dA-dT; C048773:5-isopropyl-2'-deoxyuridine; D003857:Deoxyuridine