Silencing the type II sodium channel gene: a model for neural-specific gene regulation.
Neural-specific expression of a sodium channel mini-gene has been shown to be mediated by a 28 bp silencer element, RE1, located in the 5' flanking region of the gene. This element is active exclusively in cell lines that do not express the endogenous brain type II sodium channel gene, including fibroblast, skeletal muscle, and certain neuronal cell lines. All of these non-type II expressing cells contain RE1-binding complexes. On the basis of mutational analysis and in vivo "repressor trap" experiments, we propose that cell-specific RE1-binding proteins are responsible, at least in part, for restricting expression of the type II sodium channel gene to specific neurons in the vertebrate nervous system.
Kraner
S D
SD
Department of Neurobiology and Behavior, State University of New York, Stony Brook 11794.
Chong
J A
JA
Tsay
H J
HJ
Mandel
G
G
D000310:Adrenal Gland Neoplasms / Q000473:pathology / Q000648:ultrastructure; D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D004247:DNA / Q000235:genetics; D004273:DNA, Neoplasm / Q000235:genetics; D003851:Deoxyribonucleases; D005347:Fibroblasts / Q000166:cytology / Q000648:ultrastructure; D005786:Gene Expression Regulation / Q000502:physiology*; D005809:Genes, Regulator / Q000235:genetics* / Q000502:physiology; D008969:Molecular Sequence Data; D009132:Muscles / Q000166:cytology / Q000648:ultrastructure; D009154:Mutation / Q000235:genetics; D009474:Neurons / Q000166:cytology / Q000502:physiology* / Q000648:ultrastructure; D010673:Pheochromocytoma / Q000473:pathology / Q000648:ultrastructure; D051381:Rats; D012045:Regulatory Sequences, Nucleic Acid; D015222:Sodium Channels / Q000235:genetics* / Q000502:physiology; D014162:Transfection; D014407:Tumor Cells, Cultured / Q000473:pathology / Q000648:ultrastructure
D004273:DNA, Neoplasm; D015222:Sodium Channels; D004247:DNA; D003851:Deoxyribonucleases