An amino-terminal fragment of SV40 T antigen transforms REF52 cells.
An SV40 mutant, T147D, encodes only the amino-terminal 147 amino acids of large T antigen and does not make small t antigen. We show here that a retrovirus which expresses this mutant T antigen transforms rat REF52 cells as efficiently as a retrovirus that expresses both the wild-type large and small T antigens. This cell line had previously been refractory to transformation by mutants that make short, amino-terminal fragments of T antigen.
Sompayrac
L
L
Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder 80302.
Danna
K J
KJ
D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000235:genetics / Q000502:physiology*; D002460:Cell Line; D002472:Cell Transformation, Viral; D003001:Cloning, Molecular; D009154:Mutation; D010446:Peptide Fragments / Q000235:genetics / Q000502:physiology; D051381:Rats; D013539:Simian virus 40 / Q000235:genetics; D014162:Transfection
D000952:Antigens, Polyomavirus Transforming; D010446:Peptide Fragments