Changes in the levels of rat interleukin 8/CINC and gelatinase in the exudate of carrageenin-induced inflammation in rats.
A sensitive enzyme-linked immunosorbent assay (ELISA) for rat interleukin 8/cytokine-induced neutrophil chemoattractant (CINC) has been established by using biotin-conjugated anti-CINC rabbit IgG. The biotin-streptavidin sandwich ELISA detected CINC at concentrations from 3 pg/ml up to 30 ng/ml. The concentration of CINC in the pouch fluid (exudate) of rat carrageenin-induced inflammation was measured by the ELISA. After a time lag of about 2 h, neutrophils steadily accumulated in the carrageenin/air-pouch until 8 h. Similarly, the CINC level of exudate increased after about a 2-h lag, and reached a maximum (134 ng/ml) at 8 h, and thereafter decreased to a negligible concentration at 24 h after carrageenin injection. In association with the rise in CINC level, the concentration of exudate 96-kDa gelatinase corresponding to neutrophil gelatinase/type IV collagenase increased with time. The results suggest that CINC contributes, at least in part, to the neutrophil migration into the inflammatory lesion of the carrageenin-induced inflammation in rats.
Nakagawa
H
H
Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University, Japan.
Ikesue
A
A
Kato
H
H
Debuchi
H
H
Watanabe
K
K
Tsurufuji
S
S
Naganawa
M
M
Mitamura
M
M
D000818:Animals; D002351:Carrageenan; D019743:Chemokines, CXC; D002630:Chemotactic Factors / Q000032:analysis / Q000378:metabolism* / Q000494:pharmacology; D002634:Chemotaxis, Leukocyte; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005122:Exudates and Transudates / Q000201:enzymology / Q000378:metabolism*; D018093:Gelatinases; D006133:Growth Substances / Q000032:analysis / Q000378:metabolism* / Q000494:pharmacology; D007249:Inflammation / Q000139:chemically induced / Q000201:enzymology / Q000378:metabolism*; D036341:Intercellular Signaling Peptides and Proteins; D016209:Interleukin-8 / Q000032:analysis / Q000378:metabolism*; D008297:Male; D009504:Neutrophils / Q000378:metabolism; D010434:Pepsin A / Q000032:analysis / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
D019743:Chemokines, CXC; D002630:Chemotactic Factors; D006133:Growth Substances; D036341:Intercellular Signaling Peptides and Proteins; D016209:Interleukin-8; D002351:Carrageenan; D010434:Pepsin A; D018093:Gelatinases