Evaluation of an enzyme-linked immunosorbent assay for detection of antibodies to vesicular stomatitis virus in cattle in an enzootic region of Mexico.
An ELISA was compared with the plaque-reduction serum neutralization (PRSN) test, for detection of vesicular stomatitis virus (VSV) antibodies in cattle in a vesicular stomatitis enzootic region of Mexico. A total of 325 bovine serum samples were screened for VSV antibodies. The PRSN test was performed, using Vero cells. The ELISA contained gradient-purified VSV Indiana (Lab strain) and VSV New Jersey (Hazelhurst) as the antigens. Regression analysis and weighted kappa statistic were used to estimate measures of agreement between the 2 assays for detection of VSV antibodies. The ELISA method proved useful for serodiagnosis of vesicular stomatitis. The ELISA and PRSN test results were highly correlated for detection of VSV antibodies.
Hernández De Anda
J
J
Instituto de Investigaciones en Ciencias Veterinarias, Universidad Autónoma de Baja California, México.
Salman
M D
MD
Webb
P A
PA
Keefe
T J
TJ
Arregín Arévalo
A
A
Mason
J
J
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D000956:Antigens, Viral / Q000276:immunology; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis* / Q000453:epidemiology; D004797:Enzyme-Linked Immunosorbent Assay; D005069:Evaluation Studies as Topic; D008800:Mexico / Q000453:epidemiology; D009500:Neutralization Tests; D011237:Predictive Value of Tests; D012044:Regression Analysis; D015203:Reproducibility of Results; D013280:Stomatitis / Q000175:diagnosis / Q000453:epidemiology / Q000662:veterinary*; D014709:Vero Cells; D014721:Vesicular stomatitis Indiana virus / Q000276:immunology*; D018116:Vesiculovirus; D014777:Virus Diseases / Q000175:diagnosis / Q000453:epidemiology / Q000662:veterinary*
D000914:Antibodies, Viral; D000956:Antigens, Viral