Immunoscintigraphy in Hodgkin's disease and anaplastic large cell lymphomas: results in 18 patients using the iodine radiolabeled monoclonal antibody HRS-3.
Immunoscintigraphy (IS) using the HRS-3 Hodgkin associated monoclonal antibody (MoAb) was performed in 18 patients with Hodgkin's Disease (HD) at staging or restaging. Either F(ab')2 fragments (14 patients) or whole HRS-3 (4 patients) labeled with 77-260 Mbq 131-I were used. Whole body images, including anterior and posterior views, were taken from a digital gamma camera, within 4 to 8 hours after injection and then daily for 5 days. In one patient IS was discontinued due to iodine intolerance. Seventeen patients were evaluable: 14 showed a true positive result including 2 cases which were reviewed as anaplastic large cell lymphoma (ALCL). Nodal, splenic, bone marrow and muscle involvements were imaged, many of these sites were previously unsuspected. In 7 patients with true positive findings the Pressman Specificity Index, as measured from biopsied material, ranged from 1.5-3 in 4 patients and from 5 to greater than 100 in 3 patients. Imaging was equivocal or failed in 1 patient (lymph nodes). In 2 patients, IS imaging was truly negative due to the absence of active HD, and a false negative result occurred once (inguinal node). In none of the patients a false positive image was observed. In order to rule out non-specific iodine uptake a control, anti-ACE MoAb, labeled with 125-Iodine was injected simultaneously in 10 patients. The evaluation of the study gave a sensitivity of 87% and a good specificity. IS using radioiodine labeled MoAbs is feasible and represents a reliable non-invasive diagnostic method for the staging and follow-up of HD and ALCL.
da Costa
L
L
Institut Gustave Roussy, Villejuif, France.
Carde
P
P
Lumbroso
J D
JD
Ricard
M
M
Pfreundschuh
M
M
Bosq
J
J
Manil
L
L
Diehl
V
V
Parmentier
C
C
D000818:Animals; D000911:Antibodies, Monoclonal / Q000302:isolation & purification; D015703:Antigens, CD / Q000276:immunology*; D000951:Antigens, Neoplasm / Q000276:immunology*; D004341:Drug Evaluation; D006689:Hodgkin Disease / Q000000981:diagnostic imaging*; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007454:Iodides / Q000008:administration & dosage; D007457:Iodine Radioisotopes; D017730:Ki-1 Antigen; D016403:Lymphoma, Large B-Cell, Diffuse / Q000000981:diagnostic imaging*; D051379:Mice; D008807:Mice, Inbred BALB C; D016719:Radioimmunodetection / Q000379:methods*; D012680:Sensitivity and Specificity; D012996:Solutions
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000951:Antigens, Neoplasm; D007454:Iodides; D007457:Iodine Radioisotopes; D017730:Ki-1 Antigen; D012996:Solutions; C010389:Lugol's solution