title: Concerted evolution of the mouse Tcp-10 gene family: implications for the functional basis of t haplotype transmission ratio distortion.
abstract: The mouse Tcr locus is defined by its central role in the transmission ratio distortion phenotype characteristic of t haplotypes. A molecular candidate for Tcr has been identified in the form of a gene--Tcp-10b--expressed during spermatogenesis. Tcp-10b is one member of a multigene family present in two to four copies on different homologs of chromosome 17. The coding regions of the Tcp-10 genes present within two inbred strains were compared with those of the tw5 haplotype. The various gene family members are highly conserved relative to each other with a minimum nucleotide identity of 98.6% in all pairwise comparisons. Maximal parsimony analysis indicates that the Tcp-10 gene family has evolved in a concerted manner with the obliteration of nearly all individual gene-specific characteristics. As a consequence, the candidate for the full-length mutant Tcr gene product is distinguished by only a single, highly conservative, amino acid change. The data are consistent with the hypothesis that the effector of mutant Tcr activity is a second, alternatively spliced product that is expressed in a haploid- and allele-specific manner.
authors.affiliation: Department of Molecular Biology, Princeton University, New Jersey 08544.
authors.forename: S H
authors.initials: SH
authors.lastname: Pilder
authors.forename: C L
authors.initials: CL
authors.lastname: Decker
authors.forename: S
authors.initials: S
authors.lastname: Islam
authors.forename: C
authors.initials: C
authors.lastname: Buck
authors.forename: J A
authors.initials: JA
authors.lastname: Cebra-Thomas
authors.forename: L M
authors.initials: LM
authors.lastname: Silver
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D005075:Biological Evolution; D002872:Chromosome Deletion; D002874:Chromosome Mapping; D006239:Haplotypes; D047908:Intracellular Signaling Peptides and Proteins; D008297:Male; D051379:Mice; D008869:Microtubule-Associated Proteins; D008969:Molecular Sequence Data; D005810:Multigene Family; D009687:Nuclear Proteins / Q000235:genetics*; D010641:Phenotype; D011506:Proteins; D012326:RNA Splicing; D016415:Sequence Alignment; D013735:Testicular Hormones / Q000235:genetics*; D044767:Ubiquitin-Protein Ligases; D056427:t-Complex Genome Region
chemical_list: D047908:Intracellular Signaling Peptides and Proteins; D008869:Microtubule-Associated Proteins; D009687:Nuclear Proteins; D011506:Proteins; C533392:TCP10L3 protein, human; C533395:Tcp10 protein, mouse; D013735:Testicular Hormones; C116196:PPP1R11 protein, human; D044767:Ubiquitin-Protein Ligases