title: Altered chromatin higher-order structure in cells infected by herpes simplex virus type 1.
abstract: In cells infected by herpesviruses chromosomal aberrations such as breaks and constrictions are commonly observed. Previous studies have shown that chromatin modifications in cells infected by herpes simplex virus type 1 (HSV-1) are not due to extensive breakdown of host-cell DNA or disruption of nucleosomal structure. We have previously shown that infection by HSV-1 induces single-stranded breaks in the host-cell DNA in a time-dependent fashion. Here we report that the early DNA damage observed in virus-infected cells is related to modifications in the higher-order structure of host-cell chromatin. Such modifications seem to be of a more permanent nature than the DNA single-stranded breaks.
authors.affiliation: Laboratoire d'Immunobiologie, Faculté de Médecine Paris V, France.
authors.forename: A
authors.initials: A
authors.lastname: Aranda-Anzaldo
mesh_terms: D002843:Chromatin / Q000378:metabolism; D002869:Chromosome Aberrations; D004249:DNA Damage; D006367:HeLa Cells; D006801:Humans; D009698:Nucleoproteins / Q000378:metabolism; D018139:Simplexvirus / Q000502:physiology*; D014461:Ultracentrifugation; D014466:Ultraviolet Rays
chemical_list: D002843:Chromatin; D009698:Nucleoproteins