Internalizable insulin-BSA-chlorin E6 conjugate is a more effective photosensitizer than chlorin E6 alone.
Experiments with human hepatoma PLC/PRF/5 cells involving the use of two different tests (colony formation and Trypan blue exclusion) have demonstrated a significantly higher photosensitizing activity of chlorin e6 conjugates with bovine serum albumin (BSA) and internalizable ligand insulin as compared to that of chlorin e6 itself. Receptor-mediated internalization of insulin-BSA-chlorin e6 conjugates ensures greater photosensitization of cells than the binding of those conjugates to cell surface receptors. The suitability of such conjugates permitting the delivery of a photosensitizer to most sensitive cell structures is discussed.
Sobolev
A S
AS
Russian Research-Production Center of Molecular Diagnostics and Therapy, Moscow, Russia.
Akhlynina
T V
TV
Yachmenev
S V
SV
Rosenkranz
A A
AA
Severin
E S
ES
D006528:Carcinoma, Hepatocellular; D002735:Chlorophyllides; D006801:Humans; D007328:Insulin / Q000378:metabolism*; D008113:Liver Neoplasms; D010777:Photochemistry; D011166:Porphyrins / Q000378:metabolism*; D011838:Radiation-Sensitizing Agents / Q000378:metabolism*; D012710:Serum Albumin, Bovine / Q000378:metabolism; D014407:Tumor Cells, Cultured
D002735:Chlorophyllides; D007328:Insulin; D011166:Porphyrins; D011838:Radiation-Sensitizing Agents; D012710:Serum Albumin, Bovine; C062985:phytochlorin