title: Effect of a two-solution fluoride mouthrinse on remineralization of enamel lesions in vitro.
abstract: A previous study showed that a two-solution fluoride (F) rinse deposited significantly more loosely-bound F on the tooth surface than did a sodium fluoride (NaF) rinse with the same F concentration (12 mmol/L). In the present study, this experimental rinse was evaluated for its ability to cause remineralization of enamel lesions in an in vitro pH-cycling model. Caries-like lesions were formed in the enamel of extracted human molars by means of a pH 4 demineralizing solution. Fifty-one approximately 120-microns-thick sections containing lesions were randomly divided into (1) control, (2) NaF rinse, and (3) two-solution F rinse groups. With the cut surfaces protected, the control samples were immersed in a pH 7 remineralizing solution for 12 days, and twice daily the sections were also exposed to a pH 4 demineralizing solution for 30 min. Samples in the NaF group received an additional one-minute rinse with a NaF (12 mmol/L) solution twice daily. Samples in the two-solution rinse group received the rinse treatment with a 12 mmol/L F solution prepared by combination of a Na2SiF6 and phosphate-containing solution with a calcium solution just before use. The mineral contents of the lesions were assessed by quantitative microradiography. The results showed that (1) no significant de- or remineralization was detected in the controls; (2) a 46% decrease in mineral loss (delta Z) of the lesion was produced by the NaF rinses; and (3) a 94% decrease in delta Z and a 20-microns-thick, mineral-dense surface-coating were produced by the two-solution F rinse treatment.
authors.affiliation: American Dental Association Health Foundation, Paffenbarger Research Center, National Institute of Standards and Technology, Gaithersburg, Maryland 20899.
authors.forename: L C
authors.initials: LC
authors.lastname: Chow
authors.forename: S
authors.initials: S
authors.lastname: Takagi
authors.forename: S
authors.initials: S
authors.lastname: Shih
mesh_terms: D001031:Apatites / Q000008:administration & dosage / Q000627:therapeutic use; D002124:Calcium Fluoride / Q000008:administration & dosage / Q000627:therapeutic use; D003743:Dental Enamel / Q000737:chemistry / Q000187:drug effects*; D017886:Durapatite; D005459:Fluorides / Q000008:administration & dosage / Q000627:therapeutic use*; D006801:Humans; D006882:Hydroxyapatites / Q000008:administration & dosage / Q000627:therapeutic use; D007091:Image Processing, Computer-Assisted; D008852:Microradiography; D008903:Minerals / Q000032:analysis; D009067:Mouthwashes; D011856:Radiographic Image Enhancement; D012969:Sodium Fluoride / Q000627:therapeutic use*; D017001:Tooth Demineralization / Q000503:physiopathology*; D014089:Tooth Remineralization
chemical_list: D001031:Apatites; D006882:Hydroxyapatites; D008903:Minerals; D009067:Mouthwashes; D012969:Sodium Fluoride; D017886:Durapatite; C025105:fluorapatite; D002124:Calcium Fluoride; D005459:Fluorides