Effect of aciclovir on the replication of herpes simplex virus type 1 in MA-104 cell line resistant to aciclovir.
A culture of monkey kidney cells (MA-104) resistant to aciclovir (CAS 59277-89-3) was established. The resistant cells were continuously grown in medium containing 200 mumol/l aciclovir whereas normal cells died in such medium. The doses of aciclovir required for 50 and 90% reduction in virus yield were for several Herpes simplex virus Type 1 clinical isolates up to 5-fold higher in resistant than in normal MA-104 cells; in contrast, doses required for 99% reduction in virus yield could be as much as 50-fold higher in the resistant cells. Virus yields developed in the presence of 30 mumol aciclovir in culture medium were at least 10(3)-fold higher in resistant than in normal MA-104 cells. The results showed that the antiviral effect of aciclovir may be significantly decreased in the cell line which developed resistance to aciclovir.
Cinatl
J
J
Department of Virology, Johann Wolfgang Goethe-University, Frankfurt/Main, Fed. Rep. of Germany.
Cinatl
J
J
Rabenau
H
H
Mainke
M
M
Kornhuber
B
B
Doerr
H W
HW
D000212:Acyclovir / Q000494:pharmacology*; D000818:Animals; D002460:Cell Line; D004352:Drug Resistance, Microbial; D000882:Haplorhini; D018139:Simplexvirus / Q000187:drug effects*; D014779:Virus Replication / Q000187:drug effects
D000212:Acyclovir