title: Air sampling procedure for evaluation of viral excretion level by vaccinated pigs infected with Aujeszky's disease (pseudorabies) virus.
abstract: Five groups of eight fattening pigs were vaccinated and then infected with Aujeszky's disease virus. Viral excretion was evaluated by two means: deep nasal swabbing and air sampling. It appeared that infectious airborne virus could be recovered from day 1 to day 6 after infection in the isolated units where control animals were raised. In vaccinated animals, airborne particles were also detected but the amount and duration varied in relation to their immune status at the day of virulent challenge: viral excretion was significantly lower in pigs presenting a high antibody level (1/16 to 1/64) just before infection. Results obtained with nasal swabs and with air samples were closely related. Despite its low sensitivity, the air sampling procedure could be considered as an efficient tool for reflecting infectious viral pressure in a confined atmosphere.
authors.affiliation: Ministère de l'Agriculture et de la Forêt, CNEVA, Laboratoire de Recherches Avicole et Porcine, Ploufragan, France.
authors.forename: E
authors.initials: E
authors.lastname: Bourgueil
authors.forename: E
authors.initials: E
authors.lastname: Hutet
authors.forename: R
authors.initials: R
authors.lastname: Cariolet
authors.forename: P
authors.initials: P
authors.lastname: Vannier
mesh_terms: D000391:Air Microbiology; D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D004197:Disease Reservoirs; D005069:Evaluation Studies as Topic; D011558:Herpesvirus 1, Suid / Q000276:immunology / Q000302:isolation & purification*; D009297:Nasal Mucosa / Q000382:microbiology; D011557:Pseudorabies / Q000517:prevention & control* / Q000635:transmission; D013552:Swine; D013553:Swine Diseases / Q000517:prevention & control* / Q000635:transmission; D014611:Vaccination / Q000662:veterinary; D014765:Viral Vaccines
chemical_list: D000914:Antibodies, Viral; D014765:Viral Vaccines