title: Interleukin-8 and neutrophil markers in colonic mucosa from patients with ulcerative colitis.
abstract: In this study, mediators of inflammation were characterized in colonic and terminal ileum mucosa from subjects with ulcerative colitis. We considered the role of two different chemotactic factors (interleukin-8 and leukotriene B4) and of myeloperoxidase in the pathology of inflammatory bowel disease. Serial biopsy specimens were taken at different sites, washed in 0.02 M phosphate/saline buffer, homogenized, and then sonically disrupted. In both the proximal and distal regions of the colonic mucosa of ulcerative colitis patients, there was a more than 10-fold increase in interleukin-8 levels over that in control subjects (> 300 pg/mg protein vs. 30 pg/mg protein in controls, p < or = 0.01). However, terminal ileum levels of interleukin-8 were the same in ulcerative colitis and control groups (150 pg/mg protein). There was also a 3- to 5-fold increase in leukotriene B4 levels and a several-fold increase in myeloperoxidase levels throughout the colonic mucosa in patients with ulcerative colitis. This study demonstrates that 1) interleukin-8 may have an immunoregulatory role in the pathogenesis of inflammatory bowel disease, and 2) interleukin-8, myeloperoxidase, and leukotriene B4 may be useful markers for the biochemical identification of inflammatory bowel disease.
authors.affiliation: Department of Medicine, Nassau County Medical Center, East Meadow, New York.
authors.forename: R S
authors.initials: RS
authors.lastname: Izzo
authors.forename: K
authors.initials: K
authors.lastname: Witkon
authors.forename: A I
authors.initials: AI
authors.lastname: Chen
authors.forename: C
authors.initials: C
authors.lastname: Hadjiyane
authors.forename: M I
authors.initials: MI
authors.lastname: Weinstein
authors.forename: C
authors.initials: C
authors.lastname: Pellecchia
mesh_terms: D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000032:analysis; D002374:Catalase / Q000378:metabolism; D003093:Colitis, Ulcerative / Q000175:diagnosis* / Q000276:immunology; D003106:Colon / Q000378:metabolism*; D006801:Humans; D007082:Ileum / Q000378:metabolism; D016209:Interleukin-8 / Q000032:analysis / Q000276:immunology*; D007413:Intestinal Mucosa / Q000378:metabolism*; D007975:Leukotriene B4 / Q000032:analysis / Q000276:immunology*; D008875:Middle Aged; D009504:Neutrophils / Q000276:immunology*; D009195:Peroxidase / Q000378:metabolism* / Q000502:physiology; D011863:Radioimmunoassay
chemical_list: D015415:Biomarkers; D016209:Interleukin-8; D007975:Leukotriene B4; D002374:Catalase; D009195:Peroxidase