An outbreak of Mycoplasma synoviae infection in North Carolina turkeys: comparison of isolates by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and restriction endonuclease analysis.
Mycoplasma synoviae (MS) isolates made in 1988-89 from turkey flocks in North Carolina, Missouri, and Ontario, Canada, were compared with each other and MS reference strains (WVU-1853, F10-2AS, Neb-3S, and K1968) by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of cell proteins and restriction endonuclease analysis (REA) of DNA. SDS-PAGE and REA indicated considerable homology among MS reference strains and recent field isolates. However, sufficient differences were resolved to identify the MS reference strains as different from each other and the field isolates, and to classify seven of nine recent field isolates as a cluster of nearly identical strains. The results suggest that flocks infected with members of the cluster were epizootiologically associated, possibly by a common or point source of infection.
Ley
D H
DH
Department of Food Animal and Equine Medicine, College of Veterinary Medicine, North Carolina State University, Raleigh 27606.
Avakian
A P
AP
D000818:Animals; D001426:Bacterial Proteins / Q000032:analysis; D004262:DNA Restriction Enzymes; D004196:Disease Outbreaks / Q000662:veterinary*; D004591:Electrophoresis, Polyacrylamide Gel; D009174:Mycoplasma / Q000145:classification / Q000302:isolation & purification*; D009175:Mycoplasma Infections / Q000453:epidemiology / Q000662:veterinary*; D009657:North Carolina / Q000453:epidemiology; D011201:Poultry Diseases / Q000453:epidemiology / Q000382:microbiology*; D012967:Sodium Dodecyl Sulfate; D014422:Turkeys / Q000382:microbiology*
D001426:Bacterial Proteins; D012967:Sodium Dodecyl Sulfate; D004262:DNA Restriction Enzymes