title: Immune function in pheasants experimentally infected with marble spleen disease virus.
abstract: Two experiments were conducted to evaluate the effect of marble spleen disease virus (MSDV) infection on the immune response of pheasants. In the first, 15 ring-necked pheasants were inoculated orally with cell-culture-propagated MSDV and 15 received saline (controls). On days 7, 21, and 35 postinoculation (PI), all birds received sheep erythrocytes intravenously. Hemagglutination titers to sheep erythrocytes were determined for serum samples collected weekly for 6 weeks. The virus-inoculated group had significantly (P less than 0.05) lower hemagglutination titers than the control group. In the second experiment, 30 pheasants were allotted into two groups as above. Whole blood was collected from each bird weekly for 5 weeks. The blood was cultured in microtiter plates with or without optimum concentrations of concanavalin A. Five of 10 MSDV-inoculated pheasants had significantly depressed T lymphocyte transformation on either day 7 or day 14 PI. Overall, the depression of T lymphocyte transformation was transient and mild.
authors.affiliation: Animal Disease Diagnostic Laboratory, School of Veterinary Medicine, Purdue University, West Lafayette, Indiana 47907.
authors.forename: S D
authors.initials: SD
authors.lastname: Fitzgerald
authors.forename: A L
authors.initials: AL
authors.lastname: Fitzgerald
authors.forename: W M
authors.initials: WM
authors.lastname: Reed
authors.forename: T
authors.initials: T
authors.lastname: Burnstein
mesh_terms: D000257:Adenoviridae Infections / Q000276:immunology / Q000662:veterinary*; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D000259:Aviadenovirus / Q000276:immunology*; D001715:Bird Diseases / Q000276:immunology*; D001717:Birds; D002478:Cells, Cultured; D006386:Hemagglutination Tests; D007111:Immunity, Cellular; D008213:Lymphocyte Activation; D011897:Random Allocation; D013154:Spleen / Q000473:pathology; D013158:Splenic Diseases / Q000276:immunology / Q000662:veterinary*; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D000914:Antibodies, Viral