Identification of heat shock protein 70 in the rabies virion.
We investigated a 73-kDa polypeptide (p73), a minor component of the rabies virion (HEP-Flury and ERA strains), accounting for as much as 1% of total virion proteins. Two-dimensional gel electrophoresis and immunoblotting with the antiserum against the heat shock protein 70 (hsp70) demonstrated that p73 was identical to a constitutive type of cellular hsp70. The antiserum also detected p73/hsp70 in the purified virions of other negative-stranded RNA viruses, such as vesicular stomatitis virus (New Jersey serotype), Newcastle disease virus (Miyadera strain), and influenza A virus (PR8 strain), among which, however, the contents were variable.
Sagara
J
J
Faculty of Pharmaceutical Sciences, Kyoto University, Japan.
Kawai
A
A
D015180:Electrophoresis, Gel, Two-Dimensional; D006360:Heat-Shock Proteins / Q000032:analysis* / Q000737:chemistry / Q000276:immunology; D007106:Immune Sera; D015151:Immunoblotting; D009980:Influenza A virus / Q000737:chemistry; D009522:Newcastle disease virus / Q000737:chemistry; D011820:Rabies virus / Q000737:chemistry*; D018116:Vesiculovirus / Q000737:chemistry; D014764:Viral Proteins / Q000032:analysis* / Q000737:chemistry / Q000276:immunology; D014771:Virion / Q000737:chemistry*
D006360:Heat-Shock Proteins; D007106:Immune Sera; D014764:Viral Proteins