Localization of vasoactive intestinal polypeptide-receptor-immunoreactivity in human salivary glands.
A monoclonal VIP-receptor antibody derived from a human adenocarcinoma cell line (HT 29) was used in combination with immunogold silver staining for the immunohistochemical demonstration of VIP-receptor (rec) immunoreactivity (IR) in paraffin-embedded human salivary glands (parotid, palatal, labial glands). VIP-rec-IR was localized to mucous endpieces of labial and--to a lesser extent--palatal glands, intercalated ducts of the parotid gland, and excretory ducts of all glands investigated. The findings correlate well with known effects of VIP on mucous release and electrolyte transport in salivary glands. Lack of VIP-rec-IR at serious acini may point to immunologically different receptor subtypes in these glands.
Herbst
W M
WM
Department of Dermatology, University of Erlangen-Nürnberg.
Kummer
W
W
Heym
C
C
D000911:Antibodies, Monoclonal; D006801:Humans; D007150:Immunohistochemistry; D010306:Parotid Gland / Q000737:chemistry; D011964:Receptors, Gastrointestinal Hormone / Q000032:analysis* / Q000276:immunology; D018005:Receptors, Vasoactive Intestinal Peptide; D012469:Salivary Glands / Q000737:chemistry*; D014407:Tumor Cells, Cultured; D014660:Vasoactive Intestinal Peptide
D000911:Antibodies, Monoclonal; D011964:Receptors, Gastrointestinal Hormone; D018005:Receptors, Vasoactive Intestinal Peptide; D014660:Vasoactive Intestinal Peptide