title: Metastasis-associated alterations in phospholipids and fatty acids of human prostatic adenocarcinoma cell lines.
abstract: Metastatic variants of human prostatic adenocarcinoma cell lines (DU-145, LNCaP, and ND-1) were studied by using soft agar colony forming efficiency, nude mice tumorigenicity, in vitro invasion assay, and type IV collagenase assay. The DU-145 and ND-1 cell line showed higher metastatic potential than LNCaP. Lipids from DU-145, ND-1, and LNCaP cells were extracted and analyzed by thin-layer chromatography and gas-liquid chromatography. The major lipids were phosphatidylcholine, phosphatidylethanolamine, sphingomyelin, fatty acids, and cholesterol. The sphingomyelin level was significantly higher in highly metastatic cells (DU-145 and ND-1) compared with the lower metastatic variant (LNCaP). The increase in the synthetic pathway and decrease in degradation pathway of sphingomyelin in microsomal fractions was sufficient to account for the measured increase in sphingomyelin in DU-145 cells compared with LNCaP cells. The major fatty acids of these lipids were palmitic (16:0), stearic (18:0), oelic (18:1), and arachidonic acid (20:4). The arachidonic acid level was significantly decreased in DU-145 and ND-1 compared with LNCaP cells. Electron microscopic studies showed no significant changes in the morphology of DU-145, ND-1, and LNCaP cells. The results of these investigations demonstrate for the first time that sphingomyelin and arachidonic acid contents are different in high and low metastatic variants of human prostatic adenocarcinoma cell lines.
authors.affiliation: Department of Urology, Pathology, and Cytogenetics, University of California Medical School, San Francisco 94143.
authors.forename: R
authors.initials: R
authors.lastname: Dahiya
authors.forename: B
authors.initials: B
authors.lastname: Boyle
authors.forename: B C
authors.initials: BC
authors.lastname: Goldberg
authors.forename: W H
authors.initials: WH
authors.lastname: Yoon
authors.forename: B
authors.initials: B
authors.lastname: Konety
authors.forename: K
authors.initials: K
authors.lastname: Chen
authors.forename: T S
authors.initials: TS
authors.lastname: Yen
authors.forename: W
authors.initials: W
authors.lastname: Blumenfeld
authors.forename: P
authors.initials: P
authors.lastname: Narayan
mesh_terms: D000230:Adenocarcinoma / Q000737:chemistry / Q000556:secondary*; D000818:Animals; D001095:Arachidonic Acids / Q000378:metabolism; D001932:Brain Neoplasms / Q000737:chemistry / Q000556:secondary*; D005227:Fatty Acids / Q000032:analysis*; D006801:Humans; D008207:Lymphatic Metastasis; D008297:Male; D051379:Mice; D008819:Mice, Nude; D008861:Microsomes / Q000378:metabolism; D009368:Neoplasm Transplantation; D010743:Phospholipids / Q000032:analysis*; D010770:Phosphotransferases / Q000378:metabolism; D011471:Prostatic Neoplasms / Q000473:pathology*; D013108:Sphingomyelin Phosphodiesterase / Q000378:metabolism; D013109:Sphingomyelins / Q000378:metabolism; D017855:Transferases (Other Substituted Phosphate Groups); D014407:Tumor Cells, Cultured
chemical_list: D001095:Arachidonic Acids; D005227:Fatty Acids; D010743:Phospholipids; D013109:Sphingomyelins; D010770:Phosphotransferases; D017855:Transferases (Other Substituted Phosphate Groups); C019863:ceramide cholinephosphotransferase; D013108:Sphingomyelin Phosphodiesterase