In vitro inhibition of 5-lipoxygenase metabolite, leukotriene B4, in bovine mononuclear cells by bovine viral diarrhea virus.
Bovine viral diarrhea (BVD) virus inhibited phytohemagglutinin (PHA)-, PHA plus phorbol-12-myristate-13 acetate (PMA)- or PHA plus calcium ionophore (A23187)-stimulated bovine peripheral blood mononuclear cell (PBMC) proliferation. Further, BVD-virus inhibited A23187-stimulated leukotriene B4 (LTB4) synthesis into the culture supernatants. Presence of exogenous LTB4 failed to reverse the BVD virus-induced immunosuppression. Our results suggest that BVD virus-induced immunosuppression is due to a factor that may be necessary to induce LTB4 synthesis for normal mononuclear cell proliferation.
Atluru
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D
Department of Anatomy and Physiology, Kansas State University, Manhattan 66506.
Gudapaty
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S
Xue
W
W
Gurria
F
F
Chengappa
M M
MM
McVey
D S
DS
Minocha
H C
HC
Atluru
S
S
D000818:Animals; D001094:Arachidonate 5-Lipoxygenase / Q000378:metabolism*; D000001:Calcimycin / Q000494:pharmacology; D002417:Cattle; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D001908:Diarrhea Viruses, Bovine Viral / Q000502:physiology*; D007165:Immunosuppression Therapy; D007963:Leukocytes, Mononuclear / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology; D007975:Leukotriene B4 / Q000037:antagonists & inhibitors*; D008213:Lymphocyte Activation; D008934:Mitogens / Q000494:pharmacology; D011863:Radioimmunoassay
D008934:Mitogens; D007975:Leukotriene B4; D000001:Calcimycin; D001094:Arachidonate 5-Lipoxygenase