title: Isoforms of the Na,K-ATPase are present in both axons and dendrites of hippocampal neurons in culture.
abstract: The distributions of isoforms of the Na,K-ATPase alpha subunit were determined in mature cultured hippocampal neurons and in a polarized epithelial cell line. We find that hippocampal neurons express the alpha 1 and alpha 3 isoforms in the membranes of both axons and dendrites. In contrast the alpha 1 and alpha 3 proteins are exclusively basolateral when expressed endogenously or by stable transfection in renal epithelial cells. These data suggest that epithelial cells and hippocampal neurons localize these proteins by different mechanisms. These observations contrast with those made for the vesicular stomatitis virus and the influenza glycoproteins, which are polarized in both epithelial and neuronal cells.
authors.affiliation: Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, CT 06510.
authors.forename: G
authors.initials: G
authors.lastname: Pietrini
authors.forename: M
authors.initials: M
authors.lastname: Matteoli
authors.forename: G
authors.initials: G
authors.lastname: Banker
authors.forename: M J
authors.initials: MJ
authors.lastname: Caplan
mesh_terms: D000818:Animals; D001369:Axons / Q000201:enzymology; D016764:Cell Polarity; D002478:Cells, Cultured; D003712:Dendrites / Q000201:enzymology; D004848:Epithelium / Q000201:enzymology; D005455:Fluorescent Antibody Technique; D006624:Hippocampus / Q000201:enzymology* / Q000648:ultrastructure; D066298:In Vitro Techniques; D007527:Isoenzymes / Q000378:metabolism; D051381:Rats; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*; D014162:Transfection
chemical_list: D007527:Isoenzymes; D000254:Sodium-Potassium-Exchanging ATPase