title: Predictive value of human papillomavirus DNA detection by filter hybridization and polymerase chain reaction in women with negative results of colposcopic examination.
abstract: This study focused on women who had an abnormal Papanicolaou smear and a subsequent colposcopic examination negative for a cervical squamous intraepithelial lesion. Cervical swabs obtained during the initial colposcopic examination were analyzed for human papillomavirus (HPV) DNA, and these results were correlated with the follow-up examinations. Human papillomavirus DNA was detected by dot-blot hybridization in 31 of 109 (28%) cases. The rate of subsequent biopsy-proved squamous intraepithelial lesion was 17 of 31 (55%) for the HPV-positive group compared with 9 of 78 (11%) for the HPV-negative group. The HPV-DNA detection rate by polymerase chain reaction was 46 of 109 (42%), with a rate of subsequent squamous intraepithelial lesion of 18 of 46 (39%) for the HPV-positive group, compared with 8 of 63 (13%) for the HPV-negative group. Most women had no evidence of squamous intraepithelial lesions on follow-up if the concurrent Papanicolaou smear was normal, even when HPV DNA was detected, suggesting that HPV detection per se does not necessarily correlate with subsequent squamous intraepithelial lesion.
authors.affiliation: Department of Pathology, SUNY, Stony Brook, New York 11794-8691.
authors.forename: G J
authors.initials: GJ
authors.lastname: Nuovo
authors.forename: J
authors.initials: J
authors.lastname: Moritz
authors.forename: L L
authors.initials: LL
authors.lastname: Walsh
authors.forename: P
authors.initials: P
authors.lastname: MacConnell
authors.forename: J
authors.initials: J
authors.lastname: Koulos
mesh_terms: D002584:Cervix Uteri / Q000737:chemistry / Q000473:pathology; D003127:Colposcopy; D004279:DNA, Viral / Q000032:analysis* / Q000235:genetics; D005260:Female; D006801:Humans; D017403:In Situ Hybridization / Q000592:standards*; D065006:Papanicolaou Test; D027383:Papillomaviridae / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000592:standards*; D011237:Predictive Value of Tests; D014412:Tumor Virus Infections / Q000175:diagnosis / Q000473:pathology; D002583:Uterine Cervical Neoplasms / Q000175:diagnosis / Q000473:pathology; D014626:Vaginal Smears
chemical_list: D004279:DNA, Viral