title: Ion transport in colon cancer cell cultures studied by X-ray microanalysis.
abstract: Three colon cancer cell lines (Colo 205, HT29 and T84) were investigated by X-ray microanalysis with respect to elemental composition and the effect of cAMP on the cellular concentrations of Na, K, and Cl. The cultures were not homogeneous with respect to their elemental composition, but appeared to consist of two sub-groups, low-K cells and high-K cells. In all three cell lines, the low-K cells had, in addition, higher Ca, markedly lower Cl, and somewhat lower P and S concentrations. Differences in Na and Mg concentrations were absent or not consistent. Exposure of cells to cAMP caused a decrease of the cellular Cl and K content in high-K (high-Cl) cells. Changes in Na were not significant. No difference between the three cell lines could be noted. Incubation of the cells with phorbol myristate acetate (PMA), which has been shown to down-regulate the expression of the cystic fibrosis (CF) transmembrane conductance regulator gene and thus confer CF-like characteristics on the cells, significantly decreased the response in the cellular Cl concentration to cAMP stimulation. It is concluded that cAMP initially activates predominantly the apical Cl- channel and the basolateral K+ channel.
authors.affiliation: Department of Human Anatomy, University of Uppsala, Sweden.
authors.forename: A
authors.initials: A
authors.lastname: von Euler
authors.forename: G M
authors.initials: GM
authors.lastname: Roomans
mesh_terms: D001692:Biological Transport / Q000187:drug effects; D002712:Chlorides / Q000378:metabolism*; D003110:Colonic Neoplasms / Q000378:metabolism*; D000242:Cyclic AMP / Q000494:pharmacology*; D004577:Electron Probe Microanalysis; D004602:Elements; D006801:Humans; D011188:Potassium / Q000378:metabolism*; D012964:Sodium / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014407:Tumor Cells, Cultured
chemical_list: D002712:Chlorides; D004602:Elements; D012964:Sodium; D000242:Cyclic AMP; D013755:Tetradecanoylphorbol Acetate; D011188:Potassium