title: Angiotensin II induces formation of the early growth response gene-1 protein in rat vascular smooth muscle cells.
abstract: The effect of angiotensin II (Ang II) on the early growth response gene-1 (Egr-1) mRNA, on the Egr-1 protein and on the phosphoinositide PI turnover signalling system was investigated in the presence and absence of EXP3174, a potent non-peptide Ang II receptor antagonist. Ang II induced an accumulation of 3.4 kb Egr-1 mRNA and the 80 kDa Egr-1 protein, with a maximum at 30 min and 60 min, respectively. EXP3174 blocked the Ang II-induced increase of inositol phosphates, Egr-1 mRNA and the Egr-1 protein, suggesting the involvement of the PI signalling system by the expression of the Egr-1 gene.
authors.affiliation: Medizinische Universitäts-Poliklinik, Bonn, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Sachinidis
authors.forename: P
authors.initials: P
authors.lastname: Weisser
authors.forename: Y
authors.initials: Y
authors.lastname: Ko
authors.forename: K
authors.initials: K
authors.lastname: Schulte
authors.forename: K
authors.initials: K
authors.lastname: Meyer zu Brickwedde
authors.forename: L
authors.initials: L
authors.lastname: Neyses
authors.forename: H
authors.initials: H
authors.lastname: Vetter
mesh_terms: D000804:Angiotensin II / Q000494:pharmacology*; D000818:Animals; D002478:Cells, Cultured; D004268:DNA-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics; D051766:Early Growth Response Protein 1; D005260:Female; D017874:Immediate-Early Proteins; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000378:metabolism*; D010716:Phosphatidylinositols / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D011921:Rats, Inbred WKY; D014157:Transcription Factors / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D004268:DNA-Binding Proteins; D051766:Early Growth Response Protein 1; C486110:Egr1 protein, rat; D017874:Immediate-Early Proteins; D010716:Phosphatidylinositols; D012333:RNA, Messenger; D014157:Transcription Factors; D000804:Angiotensin II