title: Effect of an essential fatty acid deficient diet on experimental infection with Trypanosoma cruzi in germfree and conventional mice.
abstract: 1. To investigate the possible role of essential fatty acid deficiency in host cell/parasite interaction, weanling germfree (GF) and conventional (CV) CFW mice maintained on an essential fatty acid-deficient (-) or a control (+) diet for 110 days were infected with Trypanosoma cruzi. 2. Blood parasitemia indicated that the disease was milder in the animals maintained on the essential fatty acid-deficient diet than in the GF and CV controls (maximum parasitemia: GF+ 33,300, GF-26,200, CV+ 17,100 and CV- 6,400 trypomastigotes/ml blood). 3. Survival 30 days after infection was 12% for GF+, 28% for GF-, 37% for CV+ and 65% for CV- mice. 4. Linoleic and arachidonic acid levels were significantly lower in animals kept on the essential fatty acid-deficient diet (GF-: 28.0 +/- 9.3, 23.4 +/- 8.6; CV-: 37.6 +/- 5.8, 19.9 +/- 3.6) than in controls (GF+: 164.4 +/- 48.8, 162.6 +/- 45.8; CV+: 147.1 +/- 26.5, 107.5 +/- 23.6) confirming the deficiency. 5. Before the infection, succinic dehydrogenase levels were higher in liver of all CV mice (4.52 micrograms phosphate/mg fresh tissue) than in GF mice (0.84 micrograms phosphate/mg fresh tissue), whereas the opposite was true for 5'-nucleotidase levels in brain and liver, respectively (GF: 2.84 and 3.18 micrograms phosphate/mg fresh tissue; CV: 1.25 and 1.54 micrograms phosphate/mg fresh tissue). 6. The disease was milder in deficient than in control animals in both the GF and CV groups on the basis of parasitemia and survival, indicating that fatty acid-deficient mice are partially protected against Chagas' disease. The mechanism underlying this phenomenon requires further investigation.
authors.affiliation: Departamento de Bioquímica-Imunologia, Universidade Federal de Minas Gerais, Belo Horizonte, Brasil.
authors.forename: C F
authors.initials: CF
authors.lastname: Santos
authors.forename: M E
authors.initials: ME
authors.lastname: Silva
authors.forename: M E
authors.initials: ME
authors.lastname: Silva
authors.forename: M E
authors.initials: ME
authors.lastname: Silva
authors.forename: J R
authors.initials: JR
authors.lastname: Nicoli
authors.forename: L C
authors.initials: LC
authors.lastname: Crocco-Afonso
authors.forename: J E
authors.initials: JE
authors.lastname: Santos
authors.forename: E A
authors.initials: EA
authors.lastname: Bambirra
authors.forename: E C
authors.initials: EC
authors.lastname: Vieira
mesh_terms: D015720:5'-Nucleotidase / Q000032:analysis / Q000187:drug effects; D000818:Animals; D001921:Brain / Q000187:drug effects / Q000201:enzymology; D001923:Brain Chemistry / Q000187:drug effects; D014355:Chagas Disease / Q000209:etiology* / Q000378:metabolism / Q000401:mortality; D004041:Dietary Fats / Q000008:administration & dosage*; D005228:Fatty Acids, Essential / Q000172:deficiency*; D005230:Fatty Acids, Nonesterified / Q000097:blood; D005260:Female; D005856:Germ-Free Life / Q000502:physiology*; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D051379:Mice; D008815:Mice, Inbred Strains; D013385:Succinate Dehydrogenase / Q000032:analysis / Q000187:drug effects; D014886:Weaning
chemical_list: D004041:Dietary Fats; D005228:Fatty Acids, Essential; D005230:Fatty Acids, Nonesterified; D013385:Succinate Dehydrogenase; D015720:5'-Nucleotidase