A new patient-like metastatic model of human small-cell lung cancer constructed orthotopically with intact tissue via thoracotomy in nude mice.
A new nude-mouse metastasizing orthotopic transplant model of human small-cell carcinoma of the lung is described. Histologically-intact human small-cell lung tumors were transplanted to the left lung of nude mice via a thoracotomy procedure we have developed. The transplanted tumors grew extensively locally and metastasized to the opposite lung, lymph nodes and other clinically-relevant sites. The results described indicate the model developed could have clinical relevance and contrasts with models of small-cell carcinoma constructed with injections of cell suspensions which result in few or no metastases.
Wang
X
X
AntiCancer, Inc., San Diego, California 92110.
Fu
X
X
Kubota
T
T
Hoffman
R M
RM
D000818:Animals; D018288:Carcinoma, Small Cell / Q000473:pathology* / Q000556:secondary*; D002455:Cell Division; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D008175:Lung Neoplasms / Q000473:pathology* / Q000556:secondary*; D008297:Male; D051379:Mice; D008819:Mice, Nude; D009362:Neoplasm Metastasis / Q000473:pathology*; D009368:Neoplasm Transplantation / Q000379:methods; D013908:Thoracotomy; D014183:Transplantation, Heterologous