[Effect of crocidolite fibers on the cathepsin B-like enzyme activity in HL-60 cells].
In a previous study, we found that crocidolite (an asbestos fiber) had an inhibitory effect on the differentiation process of HL-60 cells induced by dimethyl sulfoxide (DMSO). Here we describe the cathepsin B-like enzyme HL-60 cells and the changes of its activity during cell differentiation with or without crocidolite treatment. The cathepsin B-like enzyme in HL-60 cell extracts had almost the same characteristics and the already known cathepsin B as for the pH optima and the effects of proteinase inhibitors. The cathepsin B-like enzyme activity increased according to the cell differentiation induced by DMSO, however, its activity was depressed by crocidolite treatment.
Tsushima
H
H
Department of Hygiene, Kawasaki Medical School, Kurashiki.
Kawakami
Y
Y
Hyodoh
F
F
Ueki
A
A
D001194:Asbestos / Q000494:pharmacology*; D017638:Asbestos, Crocidolite; D002401:Cathepsin B / Q000378:metabolism*; D002454:Cell Differentiation / Q000187:drug effects*; D003864:Depression, Chemical; D006801:Humans; D014407:Tumor Cells, Cultured / Q000201:enzymology*
D017638:Asbestos, Crocidolite; D001194:Asbestos; D002401:Cathepsin B