title: [Site-specific restrictases from Bacillus thuringiensis var. Kumantoensis].
abstract: The efficiency of bacteriophages CP-54 and CP-55 plating on Bacillus thuringiensis var. kumantoensis H18 (Kum) is decreased about 10-fold as compared with the efficiency of plating on Bacillus thuringiensis var. galleriae H5 (Gal). Bacteriophages having propagated for one cycle in Kum cells might be further grown in this strain without growth restriction. Two site-specific restriction enzymes isolated from Bacillus thuringiensis var. kumantoensis were designated BtkI and BtkII. The endonuclease BtkI recognises the same nucleotide sequence CGCG in DNA as recognised by the restriction endonuclease FnuDII; BtkII recognises the same nucleotide sequence GATC as the endonuclease Sau3A.
authors.forename: R R
authors.initials: RR
authors.lastname: Azizbekian
authors.forename: B A
authors.initials: BA
authors.lastname: Rebetish
authors.forename: E M
authors.initials: EM
authors.lastname: Netyksa
authors.forename: M A
authors.initials: MA
authors.lastname: Bychkova
authors.forename: A P
authors.initials: AP
authors.lastname: Bolotin
mesh_terms: D001413:Bacillus thuringiensis / Q000201:enzymology*; D001435:Bacteriophages / Q000254:growth & development; D004262:DNA Restriction Enzymes / Q000378:metabolism*; D004269:DNA, Bacterial / Q000378:metabolism; D013379:Substrate Specificity
chemical_list: D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes
vernacular_title: Saĭtspetsificheskie restriktazy iz shtamma Bacillus thuringiensis var.Kumantoensis.