title: Fecal bile acid excretion and composition in response to changes in dietary wheat bran, fat and calcium in the rat.
abstract: The effect and possible interactive influence of different dietary amounts of wheat bran, fat and calcium on the fecal excretion, concentration and composition of bile acids was studied in Fischer-344 rats. The fecal bile acids were analyzed using gas-liquid chromatography. Dietary wheat bran increased both total bile acid excretion and fecal weight without changes in fecal bile acid concentration. The proportion of fecal hyodeoxycholic acid decreased with increasing dietary fiber, whereas that of lithocholic and deoxycholic acids increased significantly with fiber intake. The percent content of fecal chenodeoxycholic acid did not change. Increasing dietary fat led to an increase in bile acid excretion without changes in either fecal weight or bile acid concentration. In contrast, the level of dietary calcium did not affect the total excretion of bile acids. However, since calcium increased the fecal weight, it consequently diluted bile acids and decreased their fecal concentration. Dietary fat and calcium had no influence on fecal bile acid composition. There were no interactive effects of wheat bran, fat and calcium on fecal bile acids. The finding in this study that dietary fiber, fat and calcium induce significant changes in fecal bile acids may be of relevance to the potential of bile acids to promote carcinogenesis.
authors.affiliation: Department of Medicine, George Washington University Medical Center, Washington, D.C. 20037.
authors.forename: M L
authors.initials: ML
authors.lastname: Borum
authors.forename: K L
authors.initials: KL
authors.lastname: Shehan
authors.forename: H
authors.initials: H
authors.lastname: Fromm
authors.forename: S
authors.initials: S
authors.lastname: Jahangeer
authors.forename: M K
authors.initials: MK
authors.lastname: Floor
authors.forename: O
authors.initials: O
authors.lastname: Alabaster
mesh_terms: D000818:Animals; D001647:Bile Acids and Salts / Q000032:analysis*; D002136:Calcium, Dietary / Q000494:pharmacology*; D002635:Chenodeoxycholic Acid / Q000032:analysis; D003840:Deoxycholic Acid / Q000032:analysis; D004041:Dietary Fats / Q000494:pharmacology*; D004043:Dietary Fiber / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005243:Feces / Q000737:chemistry*; D008095:Lithocholic Acid / Q000032:analysis; D008297:Male; D051381:Rats
chemical_list: D001647:Bile Acids and Salts; D002136:Calcium, Dietary; D004041:Dietary Fats; D004043:Dietary Fiber; D003840:Deoxycholic Acid; D002635:Chenodeoxycholic Acid; D008095:Lithocholic Acid; C010471:hyodeoxycholic acid