[Gene expression of hepatitis viruses in the liver and hepatocarcinogenesis].
Human hepatitis B virus (HBV) carriers run an increased risk of hepatocellular carcinoma (HCC), where the expression of HBV genes play the most important role in the initial stage of hepatocarcinogenesis. As the integration of HBV DNA into the cellular DNA of HCC as well as chronic hepatitis was demonstrated very frequently, the virus-cell fusion gene was considered to be most essential for hepatocarcinogenesis. Among the virus-cell fusion genes, the X gene is known to function as a transactivator for viral and cellular genes at the time of chronic infection. One mechanism for hepatocarcinogenesis that appears particularly reasonable is transactivation of cellular oncogenes by the X-cell fusion protein. In 1990, we found a part of the amino acid sequences in the X protein to be highly homologous to functionally essential sequences in the Kunitz domain, characteristic of Kunitz-type serine protease inhibitors. It has been recently demonstrated that X protein expressed in E. coli or from the in vitro translation system binds to a specific serine protease from the liver cells. These results indicate that transactivation function of X protein may be exerted by acting as a protease inhibitor analogue to control the proteolytic pathway of cellular transcription factor(s). On the other hand, viral hepatitis resulting from viruses other than hepatitis A virus and HBV has been referred to as non-A, non-B hepatitis. In 1989, the viral genome was molecularly cloned as a positive-strand RNA having about 10 kb in size and named as hepatitis C virus (HCV). Details of genetic structure and mechanism of expression are currently under investigation at molecular level.
Koike
K
K
Department of Gene Research Cancer Institute, Tokyo, Japan.
D001483:Base Sequence; D006528:Carcinoma, Hepatocellular / Q000209:etiology* / Q000378:metabolism; D004261:DNA Replication; D004279:DNA, Viral / Q000378:metabolism; D015870:Gene Expression; D006515:Hepatitis B virus / Q000235:genetics* / Q000502:physiology; D006801:Humans; D008099:Liver / Q000382:microbiology*; D008113:Liver Neoplasms / Q000209:etiology* / Q000378:metabolism; D008969:Molecular Sequence Data; D015533:Transcriptional Activation; D014407:Tumor Cells, Cultured; D014760:Viral Fusion Proteins
D004279:DNA, Viral; D014760:Viral Fusion Proteins