[Cytochemical study of human sperm metabolism during in vitro capacitation after cryopreservation].
To study the effects of deep freezing on the energy metabolism of human spermatozoa, we investigated, by cytochemical quantitative methods, cytochrome oxidase and lactate dehydrogenase activities of fresh and frozen human spermatozoa during in vitro capacitation. Fresh and frozen human spermatozoa were incubated in Biggers, Whitten and Wittingham's medium supplemented with 15% heat-inactivated human serum. Both histoenzymological reactions can be quantitated and have been evaluated by microdensitometric method. The results indicate that human spermatozoa depend almost entirely on anaerobic glycolysis during in vitro capacitation and suggest that both aerobic and anaerobic metabolism in spermatozoa are only slightly impaired by freezing-thawing and storage.
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Istituto di Anatomia degli Animali Domestici, Università di Milano.
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D000328:Adult; D000693:Anaerobiosis; D015415:Biomarkers; D015925:Cryopreservation; D003470:Culture Media; D003576:Electron Transport Complex IV / Q000032:analysis*; D004734:Energy Metabolism; D006019:Glycolysis; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000032:analysis*; D008297:Male; D012662:Semen Preservation; D013075:Sperm Capacitation / Q000502:physiology*; D013094:Spermatozoa / Q000201:enzymology*
D015415:Biomarkers; D003470:Culture Media; D007770:L-Lactate Dehydrogenase; D003576:Electron Transport Complex IV
Studio citochimico sul metabolismo degli spermatozoi umani nel corso della capacitazione in vitro anche dopo crioconservazione.