Mapping antibody binding sites on protein antigens.
The large surfaces of protein antigens that interact with antibody-combining sites can be determined using deuterium-exchange labelling and two-dimensional 1H nuclear magnetic resonance (NMR). This technique may also be applied to other protein-protein interactions to identify key residues that contribute to the affinity.
Paterson
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Department of Microbiology, School of Medicine, University of Pennsylvania, Philadelphia 19104-6076.
D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D000941:Antigens / Q000276:immunology*; D001666:Binding Sites, Antibody; D003574:Cytochrome c Group / Q000737:chemistry* / Q000276:immunology; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008961:Models, Structural; D011487:Protein Conformation; D011506:Proteins / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D000941:Antigens; D003574:Cytochrome c Group; D011506:Proteins