title: Genetic analysis of simian virus 40 from brains and kidneys of macaque monkeys.
abstract: Simian virus 40 (SV40) was isolated from the brains of three rhesus monkeys and the kidneys of two other rhesus monkeys with simian immunodeficiency virus-induced immunodeficiency. A striking feature of these five cases was the tissue specificity of the SV40 replication. SV40 was also isolated from the kidney of a Taiwanese rock macaque with immunodeficiency probably caused by type D retrovirus infection. Multiple full-length clones were derived from all six fresh SV40 isolates, and two separate regions of their genomes were sequenced: the origin (ori)-enhancer region and the coding region for the carboxy terminus of T antigen (T-ag). None of the 23 clones analyzed had two 72-bp enhancer elements as are present in the commonly used laboratory strain 776 of SV40; 22 of these 23 clones were identical in their ori-enhancer sequences, and these had only a single 72-bp enhancer element. We found no evidence for differences in ori-enhancer sequences associated with tissue-specific SV40 replication. The T-ag coding sequence that was analyzed was identical in all clones from kidney. However, significant variation was observed in the carboxy-terminal region of T-ag in SV40 isolated from brain tissues. This sequence variation was located in a region previously reported to be responsible for SV40 host range in cultured cell lines. Thus, SV40 appears to be an opportunistic pathogen in the setting of simian immunodeficiency virus-induced immunodeficiency, similarly to JC virus in human immunodeficiency virus-infected humans, the enhancer sequence organization generally attributed to SV40 is not representative of natural SV40 isolates, and sequence variation near the carboxy terminus of T-ag may play a role in tissue-specific replication of SV40.
authors.affiliation: New England Regional Primate Research Center, Harvard Medical School, Southborough, Massachusetts 01772.
authors.forename: P O
authors.initials: PO
authors.lastname: Ilyinskii
authors.forename: M D
authors.initials: MD
authors.lastname: Daniel
authors.forename: C J
authors.initials: CJ
authors.lastname: Horvath
authors.forename: R C
authors.initials: RC
authors.lastname: Desrosiers
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000957:Antigens, Viral, Tumor / Q000235:genetics*; D001483:Base Sequence; D001921:Brain / Q000382:microbiology; D003001:Cloning, Molecular; D004742:Enhancer Elements, Genetic / Q000235:genetics; D007668:Kidney / Q000382:microbiology; D008251:Macaca / Q000382:microbiology*; D008969:Molecular Sequence Data; D009928:Organ Specificity; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D016097:Simian Acquired Immunodeficiency Syndrome / Q000235:genetics*; D013539:Simian virus 40 / Q000235:genetics* / Q000648:ultrastructure; D014412:Tumor Virus Infections / Q000235:genetics*; D014779:Virus Replication / Q000235:genetics
chemical_list: D000957:Antigens, Viral, Tumor