Cloning, expression, and nucleotide sequence of a mutant glgC gene from Escherichia coli B.
A mutant glgC gene contained in a 10.9-kb PstI fragment was cloned from the Escherichia coli B strain SG5 via colony hybridization by using a wild-type glgC probe. The altered allosteric properties of the expressed ADPglucose synthetase were found to result from the conversion of proline to serine at amino acid residue 295.
Meyer
C R
CR
Department of Biochemistry, Michigan State University, East Lansing 48824.
Ghosh
P
P
Remy
E
E
Preiss
J
J
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics*; D015870:Gene Expression; D005798:Genes, Bacterial; D051599:Glucose-1-Phosphate Adenylyltransferase; D007700:Kinetics; D008969:Molecular Sequence Data; D009154:Mutation; D009713:Nucleotidyltransferases / Q000235:genetics* / Q000378:metabolism; D010957:Plasmids; D015183:Restriction Mapping
D009713:Nucleotidyltransferases; D051599:Glucose-1-Phosphate Adenylyltransferase