A voltage-gated calcium channel is linked to the antigen receptor in Jurkat T lymphocytes.
Activation of T lymphocytes results in an increase in intracellular Ca2+ due in large part to influx of extracellular Ca2+. Using the patch clamp technique, an inward current in Jurkat T lymphocytes was observed upon depolarization from a holding potential of -90 mV but not from -60 mV. This whole-cell current was insensitive to tetrodotoxin, carried by Ba2+, and blocked by Ni2+. Occupancy of the T lymphocyte antigen receptor increased the current's magnitude. These data suggest that antigen receptor-induced Ca2+ entry in T lymphocytes may be mediated by a voltage-regulated Ca channel.
Densmore
J J
JJ
Department of Pathology, University of Virginia Health Sciences Center, Charlottesville 22908.
Szabo
G
G
Gray
L S
LS
D015220:Calcium Channels / Q000378:metabolism*; D002460:Cell Line; D006801:Humans; D008213:Lymphocyte Activation; D008564:Membrane Potentials; D009532:Nickel / Q000494:pharmacology; D011948:Receptors, Antigen, T-Cell / Q000378:metabolism*; D013601:T-Lymphocytes / Q000166:cytology / Q000378:metabolism*
D015220:Calcium Channels; D011948:Receptors, Antigen, T-Cell; D009532:Nickel