title: The monocytic cell line SKM-1 strongly expresses the myeloperoxidase gene.
abstract: A newly established human monocytic cell line, SKM-1, showed strong expression of myeloperoxidase mRNA, to the same extent as in HL-60 cells. We studied the cell morphology and myeloperoxidase expression of this cell line, which was established from a patient with myelodysplastic syndrome who had an abnormal chromosome on the upstream region of 17p13. Electron micrographs showed the cells to have a fragile and irregular cell surface. SKM-1 cells were peroxidase-positive. About 60% of myeloperoxidase (MPO) was released to the culture fluid from SKM-1 cells but only a few percent of MPO was released from HL-60 cells into the culture fluid. The predominant mRNA size of SKM-1 myeloperoxidase was 3.3 kb although there was a smaller size as well. Fluorescent in situ hybridization of MPO mRNA showed strong staining in 5% to 10% of SKM-1 cells and of bone marrow cells from patients with myelogenous leukemia, while all cells from HL-60 were positive.
authors.affiliation: Genetic Research Laboratory, SRL, Tokyo, Japan.
authors.forename: R
authors.initials: R
authors.lastname: Kawaguchi
authors.forename: Y
authors.initials: Y
authors.lastname: Hosokawa
authors.forename: A
authors.initials: A
authors.lastname: Komine
authors.forename: M
authors.initials: M
authors.lastname: Tsutsumi
authors.forename: K
authors.initials: K
authors.lastname: Hikiji
authors.forename: A
authors.initials: A
authors.lastname: Ishida-Okawara
authors.forename: K
authors.initials: K
authors.lastname: Suzuki
authors.forename: T
authors.initials: T
authors.lastname: Nakagawa
authors.forename: K
authors.initials: K
authors.lastname: Yamaguchi
mesh_terms: D000208:Acute Disease; D000368:Aged; D000754:Anemia, Refractory, with Excess of Blasts / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D015152:Blotting, Northern; D002886:Chromosomes, Human, Pair 17; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D004915:Leukemia, Erythroblastic, Acute / Q000235:genetics / Q000378:metabolism; D007951:Leukemia, Myeloid / Q000235:genetics / Q000378:metabolism; D008297:Male; D009195:Peroxidase / Q000032:analysis*; D012334:RNA, Neoplasm / Q000032:analysis*; D014407:Tumor Cells, Cultured
chemical_list: D012334:RNA, Neoplasm; D009195:Peroxidase