Unfolding of the tertiary structure of specific tRNA and ribosomal 5S RNA from plants as studied with hydroxyl radicals.
Ribosomal 5S RNA is present in all eubacterial and eukaryotic ribosomes. Despite a large amount of experimental data on the primary and secondary structures of these types of molecules, details of their tertiary structure and their precise function in protein biosynthesis are still not known. Recently we have proposed a new model for the tertiary structure of plant 5S rRNA. In this study we applied the Fe(II)-mediated cleavage reaction to test the model. The data presented here provide experimental evidence that in the 5S rRNA molecule only a few nucleotides are buried in the tertiary structure. Similar experiments performed with methionine initiator tRNA gave results which imply the difference in its structure when compared with the X-ray structure of yeast tRNAPhe.
Barciszewska
M Z
MZ
Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznań.
Barciszewski
J
J
Erdmann
V A
VA
D001482:Base Composition; D001483:Base Sequence; D004492:Edetic Acid; D007887:Fabaceae; D005290:Ferric Compounds; D005609:Free Radicals; D006878:Hydroxides; D017665:Hydroxyl Radical; D007202:Indicators and Reagents; D007502:Iron Chelating Agents; D007700:Kinetics; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010946:Plants, Medicinal; D012341:RNA, Ribosomal, 5S / Q000737:chemistry* / Q000302:isolation & purification; D012343:RNA, Transfer / Q000737:chemistry* / Q000302:isolation & purification; D012358:RNA, Transfer, Met / Q000737:chemistry; D012360:RNA, Transfer, Phe / Q000737:chemistry; D012639:Seeds
D005290:Ferric Compounds; D005609:Free Radicals; D006878:Hydroxides; D007202:Indicators and Reagents; D007502:Iron Chelating Agents; D012341:RNA, Ribosomal, 5S; D012358:RNA, Transfer, Met; D012360:RNA, Transfer, Phe; D017665:Hydroxyl Radical; D012343:RNA, Transfer; D004492:Edetic Acid; C019179:Fe(III)-EDTA