Transmissible gastroenteritis coronavirus: surface antigens induced by virulent and attenuated strains.
Three strains of the transmissible gastroenteritis virus (TGEV) possessing different degrees of pathogenicity for piglets were examined for their capacity to express M and S glycoproteins on the infected cell surface using a microwell immunoperoxidase test. These two viral glycoproteins were easily detected on the plasma membrane of 0.1% paraformaldehyde-fixed swine testis (ST) or pig kidney (RP.D) cells which were infected with high-passaged Purdue-115 and low-passaged D-52 strains and a high-passaged attenuated (188-SG) mutant of TGEV. No significant differences were found between attenuated and virulent strains with regard to the viral antigen expression on the membrane of infected cells over a 14-h period.
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Institute of Veterinary Research, Laboratory of Virology, Bach mai-Hànôi, Viêtnam.
Bernard
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Bottreau
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D000818:Animals; D000954:Antigens, Surface / Q000032:analysis*; D002460:Cell Line; D005761:Gastroenteritis, Transmissible, of Swine / Q000276:immunology*; D006023:Glycoproteins / Q000032:analysis*; D007124:Immunoenzyme Techniques; D017293:Protein S / Q000032:analysis*; D013552:Swine; D013997:Time Factors; D005760:Transmissible gastroenteritis virus / Q000276:immunology* / Q000302:isolation & purification / Q000472:pathogenicity; D014613:Vaccines, Attenuated / Q000276:immunology; D015164:Vaccines, Inactivated / Q000276:immunology; D014774:Virulence
D000954:Antigens, Surface; D006023:Glycoproteins; D017293:Protein S; D014613:Vaccines, Attenuated; D015164:Vaccines, Inactivated; C023587:protein M (glycoprotein)