Bovine coronavirus uses N-acetyl-9-O-acetylneuraminic acid as a receptor determinant to initiate the infection of cultured cells.
The importance of N-acetyl-9-O-acetylneuraminic acid (Neu5,9Ac2) as a receptor determinant for bovine coronavirus (BCV) on cultured cells was analysed. Pretreatment of MDCK I (Madin Darby canine kidney) cells with neuraminidase or acetylesterase rendered the cells resistant to infection by BCV. The receptors on a human (CaCo-2) and a porcine (LLC-PK1) epithelial cell line were also found to be sensitive to neuraminidase treatment. The susceptibility to infection by BCV was restored after resialylation of asialo-MDCK I cells with Neu5,9Ac2. Transfer of sialic acid lacking a 9-O-acetyl group was ineffective in this respect. These results demonstrate that 9-O-acetylated sialic acid is used as a receptor determinant by BCV to infect cultured cells. The possibility is discussed that the initiation of a BCV infection involves the recognition of different types of receptors, a first receptor for primary attachment and a second receptor to mediate the fusion between the viral envelope and the cellular membrane.
Schultze
B
B
Institut für Virologie, Philipps-Universität Marburg, Germany.
Herrler
G
G
D000115:Acetylesterase / Q000494:pharmacology; D000818:Animals; D001665:Binding Sites; D002417:Cattle / Q000382:microbiology*; D002462:Cell Membrane / Q000187:drug effects; D002478:Cells, Cultured; D003332:Coronaviridae / Q000378:metabolism / Q000472:pathogenicity*; D003333:Coronaviridae Infections / Q000378:metabolism*; D019158:N-Acetylneuraminic Acid; D009439:Neuraminidase / Q000494:pharmacology; D012794:Sialic Acids / Q000737:chemistry* / Q000378:metabolism*; D013045:Species Specificity
D012794:Sialic Acids; C025676:9-O-acetyl-N-acetylneuraminic acid; D000115:Acetylesterase; D009439:Neuraminidase; D019158:N-Acetylneuraminic Acid