title: Collagenolytic activity in experimental intestinal anastomoses. Differences between small and large bowel and evidence for the presence of collagenase.
abstract: Collagen degradation is thought to be an integral part of the healing sequence of intestinal anastomoses, but almost nothing is known about the enzyme activities involved. We have studied collagenolytic activities, extracted from 1 day-old intestinal anastomoses in the rat. Using either soluble type I collagen or fibrillar type I or type III collagen as a substrate, activities measured in extracts from anastomotic segments were compared to those in extracts from uninjured intestine, removed at operation: in all cases, the collagenolytic activity in anastomotic extracts was significantly higher. This increase was significantly more pronounced in large bowel than in small bowel. The activities were strongly inhibited by serum and metallo-chelating compounds. Analysis, by means of SDS-polyacrylamide gel electrophoresis, of the reaction products of the degradation of fibrillar type I collagen by the extracts revealed the presence of a multitude of fragments, amongst them TcA fragments characteristic for the activity of mammalian collagenase. Thus, the degradative capacity towards various collagen substrates is enhanced in the anastomotic area during the first postoperative period and a true mammalian collagenase is one of the enzymes present.
authors.affiliation: Department of General Surgery, Academic Hospital, University of Nijmegen, The Netherlands.
authors.forename: J W
authors.initials: JW
authors.lastname: van der Stappen
authors.forename: T
authors.initials: T
authors.lastname: Hendriks
authors.forename: H H
authors.initials: HH
authors.lastname: de Boer
authors.forename: B M
authors.initials: BM
authors.lastname: de Man
authors.forename: J J
authors.initials: JJ
authors.lastname: de Pont
mesh_terms: D000714:Anastomosis, Surgical; D000818:Animals; D003094:Collagen / Q000378:metabolism*; D003106:Colon / Q000201:enzymology* / Q000601:surgery; D004591:Electrophoresis, Polyacrylamide Gel; D007082:Ileum / Q000201:enzymology* / Q000601:surgery; D008297:Male; D003012:Microbial Collagenase / Q000378:metabolism*; D011184:Postoperative Period; D011480:Protease Inhibitors / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D013379:Substrate Specificity
chemical_list: D011480:Protease Inhibitors; D003094:Collagen; D003012:Microbial Collagenase