title: Radiolysis of aqueous solutions of guanosine-5'-monophosphate in presence of magnesium ions.
abstract: A study of the effects of Mg2+ ions on the mononucleotide, guanosine-5'-monophosphate, in the acid form (5-GMPH2) is presented, together with the effects of gamma radiation. The physical methods used to investigate these effects were Fourier Transform Infrared (FT-IR) spectroscopy and mass spectrometry (MS). From the spectral analysis it was found that the nucleotide spectra depend on the presence of Mg2+ ions. The FT-IR intensity changes, together with the mass spectra, suggest the interaction of the magnesium atom with the base guanine at the N7 site, as well as with the phosphate group. This is important, since Mg2+ is usually thought to combine only with the phosphate group. An important finding from the MS spectra was the fact that after irradiation the magnesium atom still seems to be bound to the base at the N7 site.
authors.affiliation: Laboratory of Radiation Chemistry and Biospectroscopy, National Technical University of Athens, Greece.
authors.forename: J D
authors.initials: JD
authors.lastname: Anastassopoulou
mesh_terms: D005720:Gamma Rays; D006157:Guanosine Monophosphate / Q000528:radiation effects*; D008274:Magnesium / Q000494:pharmacology*; D013058:Mass Spectrometry; D015394:Molecular Structure; D011675:Pulse Radiolysis; D011854:Radiochemistry; D013055:Spectrophotometry, Infrared
chemical_list: D006157:Guanosine Monophosphate; D008274:Magnesium