Isolation and characterization of goldfish cdk2, a cognate variant of the cell cycle regulator cdc2.
This paper reports the nucleotide and predicted amino acid sequences of the goldfish cdk2, a cognate variant of the cell cycle regulator cdc2. The predicted protein sequence shows strong homology to the other known cdk2 (88% for Xenopus and 90% for human). A monoclonal antibody against the C-terminal sequence of goldfish cdk2 recognized a 34-kDa protein in extracts from various goldfish tissues. The protein level was high in such tissues as testis and ovary containing actively dividing cells. Protein cdk2 binds to p13sucl, the fission yeast suc1+ gene product, but not to cyclin B, with which cdc2 forms a complex. The kinase activity of cdk2 increased 30-fold when oocytes matured, although its protein level did not remarkably change. Anti-cdk2 immunoprecipitates from 32P-labeled mature oocyte extracts contained a 47-kDa protein, which was not recognized by either anti-cyclin A or anti-cyclin B antibody, indicating complex formation of cdk2 with a protein other than cyclins A or B.
Hirai
T
T
Laboratory of Reproductive Biology, National Institute for Basic Biology, Okazaki, Japan.
Yamashita
M
M
Yoshikuni
M
M
Tokumoto
T
T
Kajiura
H
H
Sakai
N
N
Nagahama
Y
Y
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D001483:Base Sequence; D002453:Cell Cycle; D004247:DNA / Q000302:isolation & purification; D005260:Female; D006054:Goldfish / Q000502:physiology*; D016200:Maturation-Promoting Factor / Q000032:analysis / Q000302:isolation & purification* / Q000502:physiology; D008969:Molecular Sequence Data; D009865:Oocytes / Q000502:physiology
D000911:Antibodies, Monoclonal; D004247:DNA; D016200:Maturation-Promoting Factor