[Role of fructose-1-phosphate kinase in expression of PEP-synthase in Escherichia coli K-12].
Mutational damage of the fruK gene coding for fructose-1-phosphate kinase leads to 2-6-fold (depending on the strain) decrease in FEP synthase activity in Escherichia coli. The fruK mutants were unable to utilize lactate as well as fructose and fructose-1-phosphate, acquiring, in addition, sensitivity to mannose in their growth medium. Reversions back to FruK+ phenotype or introduction of an intact fruK allele resulted in restoration of both FEP synthase activity and the ability to grow on lactate.
Molchanova
M L
ML
Erdagaeva
R S
RS
Grigorenko
Iu A
IuA
Bol'shakova
T N
TN
Gershanovich
V N
VN
D000483:Alleles; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005632:Fructose / Q000378:metabolism; D005636:Fructosephosphates / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D005815:Genetic Code / Q000235:genetics; D007773:Lactates / Q000378:metabolism; D019344:Lactic Acid; D008358:Mannose / Q000378:metabolism; D009154:Mutation / Q000235:genetics; D010641:Phenotype; D010732:Phosphofructokinase-1 / Q000235:genetics*; D011772:Pyruvate, Orthophosphate Dikinase / Q000502:physiology*
D005636:Fructosephosphates; D007773:Lactates; C032284:fructose-1-phosphate; D005632:Fructose; D019344:Lactic Acid; D010732:Phosphofructokinase-1; C068652:1-phosphofructokinase; D011772:Pyruvate, Orthophosphate Dikinase; D008358:Mannose
Rol' fruktozo-1-fosfatkinazy v ékspressii FEP-sintetazy u Escherichia coli K-12.