Towards identification of cis-acting elements involved in the replication of enterovirus and rhinovirus RNAs: a proposal for the existence of tRNA-like terminal structures.
On the basis of a comparative analysis of published sequences, models for the secondary structure of the 3'-terminal [poly(A)-preceding] untranslated region of the entero- and rhinovirus RNAs were worked out. The models for all these viruses share a common core element, but there are an extra enterovirus-specific element and still an additional element characteristic of a subset of enterovirus RNAs. The two latter models were verified for poliovirus and coxsackievirus B genomes by testing with single-strand and double-strand specific enzymatic and chemical probes. A tRNA-like tertiary structure model for the 3'-terminal folding of enterovirus RNAs was proposed. A similar folding was proposed for the 3' termini of the negative RNA strands as well as for the 5' termini of the positive strand of all entero- and rhinovirus RNAs. Implications of these data for template recognition during negative and positive RNA strands synthesis and for the evolution of the picornavirus genomes are discussed.
Pilipenko
E V
EV
Institute of Poliomyelitis and Viral Encephalitides, USSR Academy of Medical Sciences, Moscow.
Maslova
S V
SV
Sinyakov
A N
AN
Agol
V I
VI
D001483:Base Sequence; D005075:Biological Evolution; D004770:Enterovirus / Q000235:genetics*; D016678:Genome; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012343:RNA, Transfer / Q000737:chemistry / Q000235:genetics; D012367:RNA, Viral / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics; D012229:Rhinovirus / Q000235:genetics*
D012367:RNA, Viral; D012343:RNA, Transfer