title: Kinetics of amide proton exchange in parvalbumin studied by 1H 2-D NMR. A comparison of the calcium and magnesium loaded forms.
abstract: The amide proton exchange rates have been measured for the pike parvalbumin loaded either with calcium (PaCa2) or with magnesium (PaMg2) by using 2-D total correlation spectroscopy experiments. The differences in the exchange rates observed between these two species were unexpected when compared with the small conformational changes induced in parvalbumin by the Ca/Mg exchange. With the calcium-loaded protein (PaCa2), a significant difference was observed for the amide proton exchange rates of residues located in the N-terminal domain AB in contrast to the slower exchange rates that were observed in the CD and EF domains. Such a difference does not exist for PaMg2, where faster exchange rates are observed over all the sequence. Since amide proton exchange rates are the signature of the solvent's accessibility in proteins, we interpreted our results in terms of difference of the equilibria between 'closed-states' and 'opened-states' for individual amide protons of the protein when calcium was replaced by magnesium. The CD and EF domains, and to a lesser extent the AB domain, would be more rigid when the protein was loaded with calcium ions. For the magnesium-loaded parvalbumin (PaMg2) the faster exchange rates we observed could be rationalized by a more flexible structure than in the case of the PaCa2.
authors.affiliation: Centre CNRS-INSERM de Pharmacologie-Endocrinologie, Montpellier, France.
authors.forename: C
authors.initials: C
authors.lastname: Baldellon
authors.forename: A
authors.initials: A
authors.lastname: Padilla
authors.forename: A
authors.initials: A
authors.lastname: Cavé
mesh_terms: D000577:Amides / Q000737:chemistry*; D002118:Calcium / Q000737:chemistry*; D007700:Kinetics; D008274:Magnesium / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D010320:Parvalbumins / Q000737:chemistry*; D017434:Protein Structure, Tertiary; D011522:Protons
chemical_list: D000577:Amides; D010320:Parvalbumins; D011522:Protons; D008274:Magnesium; D002118:Calcium