Comparison of modified HAVAB and ELISA for determination of vaccine-induced anti-HAV response.
An inhibition ELISA was compared with a modification of the HAVAB assay for measuring antibodies induced by a killed HAV vaccine. GMT's expressed in mIU/ml were higher by ELISA than by modified HAVAB, especially after the first and second doses of vaccine but seroconversion rates were very similar and a good correlation was found between both assays. Because of its higher sensitivity and specificity, the ELISA assay was preferred to modified HAVAB for the evaluation of a Hepatitis A vaccine in human volunteers.
Delem
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SmithKline Beecham Biologicals, Rixensart, Belgium.
D000328:Adult; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005069:Evaluation Studies as Topic; D035922:Hepatitis A Antibodies; D022362:Hepatitis A Vaccines; D006508:Hepatitis Antibodies / Q000032:analysis* / Q000096:biosynthesis; D006507:Hepatovirus / Q000276:immunology; D006801:Humans; D011863:Radioimmunoassay / Q000379:methods*; D013997:Time Factors; D014761:Viral Hepatitis Vaccines / Q000008:administration & dosage / Q000494:pharmacology*
D035922:Hepatitis A Antibodies; D022362:Hepatitis A Vaccines; D006508:Hepatitis Antibodies; D014761:Viral Hepatitis Vaccines