Isolation and characterization of microtubule-associated protein 2 (MAP2) kinase from rat brain.
Microtubule-associated protein 2 (MAP2) kinase has been isolated and characterized from rat brain. The enzyme has an apparent M(r) of approximately 42,000 and its pI is 4.9. MAP2 was the preferred substrate, but it also phosphorylated myelin basic protein (MBP), histone V-S, tubulin and the PC12 protein substrate pp250. The enzyme is distinct from protein kinase C, cAMP-dependent kinase and the calcium/calmodulin-dependent kinases, as specific inhibitors of these kinases did not affect MAP2 phosphorylation. The addition of the relatively non-specific protein kinase inhibitor H7 (20 microM) had a modest inhibitory effect. The enzyme was active in both 5 mM Mn2+ and Mg2+, and displayed Kms for MAP2, MBP, and ATP of 56 nM, 254 nM, and 4 microM, respectively. This enzyme, which represents a low abundance protein in whole brain, is analogous to the MAP2 kinase observed in growth factor-stimulated cell lines.
Schanen
N C
NC
Department of Neurology, Case Western Reserve University Medical School, Cleveland, OH 44106.
Landreth
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G
D000818:Animals; D001921:Brain / Q000201:enzymology*; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D007700:Kinetics; D008274:Magnesium / Q000494:pharmacology; D008345:Manganese / Q000494:pharmacology; D009363:Neoplasm Proteins / Q000302:isolation & purification; D009419:Nerve Tissue Proteins / Q000302:isolation & purification*; D016716:PC12 Cells / Q000201:enzymology; D047428:Protein Kinase Inhibitors; D011494:Protein Kinases / Q000302:isolation & purification*; D051381:Rats; D013379:Substrate Specificity
D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D047428:Protein Kinase Inhibitors; D008345:Manganese; D011494:Protein Kinases; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D008274:Magnesium