PA-III rat prostate adenocarcinoma cells (review).
PA-III is one of the four transplantable cell lines propagated in vitro in cell monolayer cultures from four spontaneously developed prostate cancer tumors in aged germfree and conventional Lobund-Wistar (L-W) rats (Pollard Model). Our data documented the presence of IGF-I and glucocorticoid but the absence of IGF-II, insulin and androgen receptors in PA-III cells. Activation of IGF-I receptors by IGF-I, IGF-II and insulin stimulated the proliferation of PA-III cells. Activation of glucocorticoid receptors by dexamethasone inhibited the proliferation of PA-III cells. PA-III cells contained urokinase-like activity and expressed the mRNA for urokinase gene which was negatively regulated by glucocorticoids. The above results suggest that PA-III cells could be a useful model for the study of glucocorticoid effects on prostate cancer cells. In addition, these data are discussed in relationship to the capacity of PA-III cells to produce the osteoblastic reaction when transplanted on L-W rat skeleton. A model outlining the putative paracrine interactions between PA-III cells and rat osteoblasts is proposed.
Koutsilieris
M
M
Hormonal Bioregulation Laboratory, Laval University Hospital Center, Faculty of Medicine, Laval University, Quebec, Canada.
D000230:Adenocarcinoma / Q000737:chemistry / Q000150:complications / Q000473:pathology*; D000818:Animals; D002460:Cell Line; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006133:Growth Substances / Q000494:pharmacology; D006728:Hormones / Q000494:pharmacology; D008297:Male; D009363:Neoplasm Proteins / Q000032:analysis*; D010006:Osteoblasts / Q000473:pathology; D010014:Osteolysis / Q000209:etiology; D011471:Prostatic Neoplasms / Q000737:chemistry / Q000150:complications / Q000473:pathology*; D051381:Rats; D011956:Receptors, Cell Surface / Q000032:analysis*; D014407:Tumor Cells, Cultured / Q000737:chemistry / Q000187:drug effects / Q000473:pathology
D006133:Growth Substances; D006728:Hormones; D009363:Neoplasm Proteins; D011956:Receptors, Cell Surface