title: Plasmid profiles of Acinetobacter and Enterobacter species of hospital origin: restriction endonuclease analysis of plasmid DNA and transformation of Escherichia coli by R plasmids.
abstract: A total of 37 multi-resistant strains (20 Acinetobacter calcoaceticus and 17 Enterobacter cloacae) were isolated from patients of the Intensive Care Units. All the isolates were examined for resistance to a battery of antimicrobial agents by the disk diffusion method. Plasmid profiles and restriction endonuclease analysis of plasmid DNA by EcoR1 revealed the spread of one A. calcoaceticus and two E. cloacae endemic strains. Transformation experiments on Escherichia coli competent cells by three plasmids established the presence of R plasmids in the multi-resistant isolates.
authors.affiliation: Department of Microbiology, School of Medicine, University of Patras, Greece.
authors.forename: I
authors.initials: I
authors.lastname: Spiliopoulou
authors.forename: A
authors.initials: A
authors.lastname: Droukopoulou
authors.forename: A
authors.initials: A
authors.lastname: Athanassiadou
authors.forename: G
authors.initials: G
authors.lastname: Dimitracopoulos
mesh_terms: D000150:Acinetobacter / Q000145:classification / Q000187:drug effects / Q000235:genetics*; D003428:Cross Infection / Q000382:microbiology*; D004262:DNA Restriction Enzymes / Q000378:metabolism*; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics*; D015246:Deoxyribonuclease EcoRI / Q000378:metabolism; D004352:Drug Resistance, Microbial / Q000235:genetics; D004754:Enterobacter / Q000145:classification / Q000187:drug effects / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D008826:Microbial Sensitivity Tests; D011815:R Factors / Q000235:genetics*; D014169:Transformation, Bacterial / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes; D015246:Deoxyribonuclease EcoRI