Calbindin-D28K-containing neurons in animal models of neurodegeneration: possible protection from excitotoxicity.
Brain levels of the calcium binding protein Calbindin-D28K (CaBP28K) and CaBP28K mRNA were measured for various animal models of neurodegenerative diseases (MPTP-treated C57BL/6J mice and Sprague-Dawley rats receiving striatal/intraperitoneal kainic acid or quinolinic acid into the nucleus basalis magnocellularis). Brain areas were tested (radioimmunoassay, Western blot, slot blot, and Northern blot) for levels of CaBP28K and CaBP28K mRNA. The various models did not exhibit any changes in protein or mRNA levels from the controls, suggesting that CaBP28K-containing neurons were not lost after exposure to these neurotoxins. Immunocytochemical characterization of the substantia nigra of the MPTP-treated mice revealed that there was significant dopaminergic cell loss in this brain area after MPTP treatment. The majority of dopaminergic neurons that degenerated did not contain CaBP28K. The small percentage of surviving neurons were CaBP28K-positive. These results suggest that the presence of CaBP28K may protect neurons from calcium-mediated neurotoxicity.
Iacopino
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Department of Biochemistry and Molecular Biology, University of Medicine and Dentistry of New Jersey, Newark.
Christakos
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German
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Sonsalla
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D000818:Animals; D001480:Basal Ganglia Diseases / Q000139:chemically induced / Q000378:metabolism*; D001679:Biogenic Amines / Q000032:analysis; D064092:Calbindin 1; D064026:Calbindins; D002118:Calcium / Q000502:physiology*; D002470:Cell Survival; D004195:Disease Models, Animal; D007608:Kainic Acid / Q000633:toxicity; D020267:MPTP Poisoning; D051379:Mice; D008810:Mice, Inbred C57BL; D009410:Nerve Degeneration; D009419:Nerve Tissue Proteins / Q000096:biosynthesis / Q000502:physiology*; D009474:Neurons / Q000737:chemistry / Q000502:physiology*; D009498:Neurotoxins / Q000037:antagonists & inhibitors*; D017378:Quinolinic Acid; D011805:Quinolinic Acids / Q000633:toxicity; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains; D064030:S100 Calcium Binding Protein G / Q000502:physiology*
D001679:Biogenic Amines; C576499:Calb1 protein, mouse; C576500:Calb1 protein, rat; D064092:Calbindin 1; D064026:Calbindins; D009419:Nerve Tissue Proteins; D009498:Neurotoxins; D011805:Quinolinic Acids; D012333:RNA, Messenger; D064030:S100 Calcium Binding Protein G; D017378:Quinolinic Acid; D007608:Kainic Acid; D002118:Calcium