Effects of 17 beta-N,N-diethylcarbamoyl-4-methyl-4-aza-5 alpha-androstan-3-one on steroidogenesis in gonads of rat fetuses.
1. The effect of 5 alpha-reductase inhibitor 17 beta-N,N-diethylcarbamoyl-4-methyl-4-aza-5 alpha-androstan-3-one (4-MA) on the fetal rat steroidogenesis was examined. 18- and 20-day-old fetal ovaries were incubated in the presence of dcAMP (1 mM) and spironolactone (10 mM) and progesterone production was assessed with and without 4-MA (0.01-50 microM). 2. High concentrations of 4-MA significantly decreased the production of progesterone. 3. Similar inhibition of the testosterone synthesis was observed in 16-day-old fetal testes explanted either in the control medium (M199) or in the presence of LH (100 ng/ml) when treated with 4-MA. The effect was rapidly reversible in both gonads.
Gangnerau
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Laboratoire de Physiologie du Développement, Université Paris, France.
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D000726:Androgen Antagonists / Q000494:pharmacology*; D000818:Animals; D001378:Azasteroids / Q000494:pharmacology*; D042944:Cholestenone 5 alpha-Reductase; D000242:Cyclic AMP / Q000494:pharmacology; D013196:Dihydrotestosterone / Q000031:analogs & derivatives* / Q000494:pharmacology; D005260:Female; D005333:Fetus; D006066:Gonads / Q000187:drug effects* / Q000196:embryology / Q000378:metabolism; D008297:Male; D010053:Ovary / Q000187:drug effects; D010088:Oxidoreductases / Q000037:antagonists & inhibitors; D011374:Progesterone / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D013148:Spironolactone / Q000494:pharmacology; D013737:Testis / Q000187:drug effects; D013739:Testosterone / Q000378:metabolism*
D000726:Androgen Antagonists; D001378:Azasteroids; D013196:Dihydrotestosterone; D013148:Spironolactone; D013739:Testosterone; D011374:Progesterone; C031563:17-N,N-diethylcarbamoyl-4-methyl-4-azaandrostane-3-one; D000242:Cyclic AMP; D010088:Oxidoreductases; D042944:Cholestenone 5 alpha-Reductase