title: Insertion of SMRV-H viral DNA at the c-myc gene locus of a BL cell line and presence in established cell lines.
abstract: Mutation of the c-myc gene locus has occurred during in vitro culture of the Burkitt lymphoma cell line Namalwa. Gene cloning and sequencing of this c-myc gene locus has revealed the insertion of incomplete copies of the Squirrel Monkey Retrovirus-Human (SMRV-H) proviral genome. Insertions of the SMRV-H genome were seen in all Namalwa cell lines tested, with multiple insertions being seen in some sublines. Viral particles with the morphology of type-D retrovirus, budding from these cells, were seen by transmission EM. New insertions of the genome were produced spontaneously in these cells. The origins of the type-D retrovirus and its implications for workers in the field are discussed.
authors.affiliation: LRF Lab, Medical School, Newcastle upon Tyne, UK.
authors.forename: P G
authors.initials: PG
authors.lastname: Middleton
authors.forename: S
authors.initials: S
authors.lastname: Miller
authors.forename: J A
authors.initials: JA
authors.lastname: Ross
authors.forename: C M
authors.initials: CM
authors.lastname: Steel
authors.forename: K
authors.initials: K
authors.lastname: Guy
mesh_terms: D001483:Base Sequence; D002051:Burkitt Lymphoma / Q000235:genetics / Q000382:microbiology*; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004251:DNA Transposable Elements; D004279:DNA, Viral / Q000032:analysis*; D016259:Genes, myc; D006801:Humans; D008242:Lysogeny; D008969:Molecular Sequence Data; D015301:Retroviruses, Simian / Q000235:genetics*; D014407:Tumor Cells, Cultured
chemical_list: D004251:DNA Transposable Elements; D004279:DNA, Viral