Adenosine triphosphate content of Mycobacterium leprae by percoll buoyant density centrifugation.
Thirty nine untreated patients of bacilliferous leprosy with a mean bacteriological index of 4.8 and morphological index of 1.3% formed the study group. Adenosine triphosphate assay was carried out by (i) enzyme treatment method in 18 patients and (ii) percoll buoyant density gradient method in 21 patients. ATP content obtained by percoll buoyant density gradient method was significantly higher than that obtained by enzyme treatment method. Percoll buoyant density centrifugation for purification and isolation of bacilli from human leproma is simplier, quicker and can serve as an alternate method of enzyme treatment.
Sharma
V K
VK
Department of Dermatology, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
Kaur
S
S
Kaur
I
I
Thakur
M
M
Vaishnavi
C
C
Kohli
M
M
Ganguly
N K
NK
D000255:Adenosine Triphosphate / Q000032:analysis*; D002499:Centrifugation, Density Gradient; D006801:Humans; D015439:Leprosy, Borderline / Q000382:microbiology; D015440:Leprosy, Lepromatous / Q000382:microbiology; D009166:Mycobacterium leprae / Q000737:chemistry* / Q000302:isolation & purification; D011205:Povidone; D012822:Silicon Dioxide
C016039:Percoll; D012822:Silicon Dioxide; D000255:Adenosine Triphosphate; D011205:Povidone