Dihydroergotoxine modulation of the GABAA receptor-associated Cl- ionophore in mouse brain.
Dihydroergotoxine non-competitively displaced the binding of t-[3H]butylbicycloorthobenzoate ([3H]TBOB) to crude synaptosomal membranes from the mouse brain (cerebrum minus cortex), and gamma-aminobutyric acid (GABA) (10 microM) enhanced the displacement potency of dihydroergotoxine in a bicuculline-sensitive manner. The same ergot compound prolonged pentobarbital-induced sleeping in mice and diminished the convulsive potency of picrotoxin in the same animal species. The results are indicative of the positive coupling between GABA and dihydroergotoxine.
Tvrdeić
A
A
Ruder Bosković Institute, Laboratory for Molecular Neuropharmacology, Zagreb, Croatia.
Pericić
D
D
D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D001643:Bridged Bicyclo Compounds / Q000378:metabolism; D019086:Bridged Bicyclo Compounds, Heterocyclic; D018118:Chloride Channels; D004088:Dihydroergotoxine / Q000494:pharmacology*; D004357:Drug Synergism; D005260:Female; D007476:Ionophores; D008565:Membrane Proteins / Q000187:drug effects*; D051379:Mice; D008808:Mice, Inbred CBA; D010424:Pentobarbital / Q000494:pharmacology; D010852:Picrotoxin; D011963:Receptors, GABA-A / Q000187:drug effects* / Q000378:metabolism
D001643:Bridged Bicyclo Compounds; D019086:Bridged Bicyclo Compounds, Heterocyclic; D018118:Chloride Channels; D004088:Dihydroergotoxine; D007476:Ionophores; D008565:Membrane Proteins; D010424:Pentobarbital; D010852:Picrotoxin; D011963:Receptors, GABA-A; C046308:tert-butylbicyclo-2-benzoate