Identification and characterization of a 30K glycoprotein of herpes simplex virus type 2.
A herpes simplex virus type 2 (HSV-2) type-specific monoclonal antibody (MAb), CH-A9, precipitated a glycoprotein with an M(r) of approximately 30,000 (g30K) from extracts of HSV-2-infected BHK cells labelled with [3H]leucine, [14C]fructose or [3H]glucosamine. The M(r) of this glycoprotein is lower than those of other HSV glycoproteins. Immunoassays of BHK cells infected with HSV-1-HSV-2 intertypic recombinants localized the gene encoding the target antigen of MAb CH-A9 to the unique long (UL) region at map units 0.490 to 0.564. Tunicamycin effectively inhibits N-linked glycosylation of g30K, which suggests that g30K may be modified by addition of N-linked oligosaccharides and that the amino acid sequence may contain Asn-X-Ser or Asn-X-Thr. The g30K was also purified on an immunoadsorbent column consisting of MAb CH-A9 linked to Sepharose 4B and was shown to be an HSV-2 type-specific antigen by indirect ELISA. The glycoprotein could induce HSV-2 type-specific neutralizing antibody in BALB/c mice. This evidence suggests that g30K may be a novel glycoprotein of HSV-2.
Qin
K F
KF
Department of Microbiology, Fourth Military Medical University, Xian, Shaanxi, China.
Yu
Q G
QG
Gao
Q
Q
Wang
M X
MX
Jiang
S Z
SZ
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D005260:Female; D005814:Genes, Viral; D006023:Glycoproteins / Q000032:analysis*; D007114:Immunization; D051379:Mice; D008807:Mice, Inbred BALB C; D011233:Precipitin Tests; D018139:Simplexvirus / Q000737:chemistry* / Q000235:genetics / Q000276:immunology; D014415:Tunicamycin / Q000494:pharmacology; D014764:Viral Proteins / Q000032:analysis* / Q000276:immunology
D000911:Antibodies, Monoclonal; D006023:Glycoproteins; D014764:Viral Proteins; D014415:Tunicamycin