Altered surface antigen expression on peripheral blood mononuclear cells in cats infected with feline immunodeficiency virus.
Expression of CD4, CD8, IL-2 receptor alpha chain (IL-2R alpha), and MHC class II (MHC-II) on peripheral blood mononuclear cells were examined in cats infected with feline immunodeficiency virus (FIV). CD4/CD8 T cell ratio in FIV-infected cats was slightly decreased, as compared with that in specific-pathogen-free (SPF) cats. However, there was no statistical differences between them. The number of circulating IL-2R alpha+ cells in FIV-infected cats was higher than that in healthy cats, whereas induction of IL-2R alpha expression by concanavalin A (Con A) stimulation was depressed in FIV-infected cats. By using two-color cytofluorometry, Con A-induced enhancement of IL-2R alpha expression was found to be reduced in both CD4+ and CD8+ populations in PBMC from FIV-infected cats. The circulating MHC-II+ cells were also increased in FIV-infected cats. Furthermore, the induction of IL-2R alpha expression on PBMC after Con A-stimulation significantly depressed by FIV inoculation in vitro. These results suggest that FIV activates PBMC in vivo via direct and/or indirect mechanisms, leading to the unresponsive state of T cells to further stimuli in vitro.
Ohno
K
K
Department of Veterinary Internal Medicine, Faculty of Agriculture, University of Tokyo, Japan.
Watari
T
T
Goitsuka
R
R
Tsujimoto
H
H
Hasegawa
A
A
D000818:Animals; D000954:Antigens, Surface / Q000096:biosynthesis*; D015704:CD4 Antigens / Q000096:biosynthesis; D016516:CD4-CD8 Ratio; D016827:CD8 Antigens / Q000096:biosynthesis; D002415:Cats; D016181:Feline Acquired Immunodeficiency Syndrome / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D000949:Histocompatibility Antigens Class II / Q000096:biosynthesis; D016089:Immunodeficiency Virus, Feline / Q000276:immunology*; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D015375:Receptors, Interleukin-2 / Q000096:biosynthesis; D013047:Specific Pathogen-Free Organisms
D000954:Antigens, Surface; D015704:CD4 Antigens; D016827:CD8 Antigens; D000949:Histocompatibility Antigens Class II; D015375:Receptors, Interleukin-2