[Method of producing fusarin C in perlite-liquid culture medium].
For researching the biosynthesis labelled Fusarin C(Fc) by Fuscarium moniliforme, a more quick and convenient method of Fusarin C production and purification were established, and a good liquid culture medium consisted of different kinds organic matters (hydroxy proline, sucrose and glycerin), inorganic salts and perlite replaced corn grit medium. The perlite-liquid culture medium inoculated with the strain of F. moniliforme yields 936mg Fc/kg organic matter with in 14 days of incubation at 28 degrees C. As compared with the corn grit medium, the amount of Fc from perlite-liquid medium was more than that from corn grit medium (831mg Fc/kg corn grit). In all experiments both thin-layer chromatography and high-pressure liquid chromatography were used to confirm the presence of Fc. parameters which were important for the optimal biosynthesis of Fc included hydroxy proline and sucrose concentrations, incubated time/temperature and amount of perlite. The 40g of sucrose/L liquid culture was optimal concentration for Fusarin C production. Of three contained N-matter tested, hydroxy proline was the best sources of N-atom for Fusarin C. Under the absence of hydroxy proline, the Fc wasn't synthesized in perlite-liquid culture medium by F. moniliforme. A culture time/temperature study of Fc production was done, and the optimal Fc amounts was synthesized after incubation for 14 days at 28 degrees C on perlite-liquid culture medium.
Li
G
G
Cancer Institute, Chinese Academy of Medical Sciences, Beijing.
Li
M
M
Ma
J
J
D000537:Aluminum Oxide; D003470:Culture Media; D005670:Fusarium / Q000378:metabolism*; D011090:Polyenes / Q000302:isolation & purification*; D012822:Silicon Dioxide
D003470:Culture Media; D011090:Polyenes; C003076:Perlite; D012822:Silicon Dioxide; C041403:fusarin C; D000537:Aluminum Oxide