title: Analysis of ras oncogene mutations in human squamous cell carcinoma of the head and neck.
abstract: The presence of proto-oncogene mutations at codons 12, 13 and 61 of the Ha-, Ki-, and N-ras in primary head and neck squamous cell carcinoma are analysed in this study. Oncogene ras-specific sequences were amplified by the polymerase chain reaction and probed with mutation specific oligonucleotide probes. Mutations were detected in 8 of 22 samples (36.3%). No mutations were detected on patients' peripheral blood DNA. We found that histologically and clinically, squamous cell carcinomas with or without a ras mutation do not differ significantly from each other.
authors.affiliation: Department of Otolaryngology, Hospital Central de Asturias, Oviedo, Spain.
authors.forename: F
authors.initials: F
authors.lastname: Núñez
authors.forename: O
authors.initials: O
authors.lastname: Domínguez
authors.forename: E
authors.initials: E
authors.lastname: Coto
authors.forename: C
authors.initials: C
authors.lastname: Suárez-Nieto
authors.forename: P
authors.initials: P
authors.lastname: Pérez
authors.forename: C
authors.initials: C
authors.lastname: López-Larrea
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D016009:Chi-Square Distribution; D004247:DNA / Q000235:genetics; D011905:Genes, ras / Q000235:genetics*; D006258:Head and Neck Neoplasms / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D006801:Humans; D009154:Mutation / Q000235:genetics*; D009367:Neoplasm Staging; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction; D000090063:Proto-Oncogene Mas; D013030:Spain / Q000453:epidemiology; D016019:Survival Analysis
chemical_list: C000715470:MAS1 protein, human; D015345:Oligonucleotide Probes; D000090063:Proto-Oncogene Mas; D004247:DNA