The Entner-Doudoroff pathway in Escherichia coli is induced for oxidative glucose metabolism via pyrroloquinoline quinone-dependent glucose dehydrogenase.
The Entner-Doudoroff pathway was shown to be induced for oxidative glucose metabolism when Escherichia coli was provided with the periplasmic glucose dehydrogenase cofactor pyrroloquinoline quinone (PQQ). Induction of the Entner-Doudoroff pathway by glucose plus PQQ was established both genetically and biochemically and was shown to occur in glucose transport mutants, as well as in wild-type E. coli. These data complete the body of evidence that proves the existence of a pathway for oxidative glucose metabolism in E. coli. PQQ-dependent oxidative glucose metabolism provides a metabolic branch point in the periplasm; the choices are either oxidation to gluconate followed by induction of the Entner-Doudoroff pathway or phosphotransferase-mediated transport. The oxidative glucose pathway might be important for survival of enteric bacteria in aerobic, low-phosphate, aquatic environments.
Fliege
R
R
School of Biological Sciences, University of Nebraska, Lincoln 68588-0118.
Tong
S
S
Shibata
A
A
Nickerson
K W
KW
Conway
T
T
D001693:Biological Transport, Active / Q000187:drug effects / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D042843:Glucose 1-Dehydrogenase; D005948:Glucose Dehydrogenases / Q000378:metabolism*; D009154:Mutation; D010084:Oxidation-Reduction; D045542:PQQ Cofactor; D010710:Phosphates / Q000494:pharmacology; D015363:Quinolones / Q000494:pharmacology
D010710:Phosphates; D015363:Quinolones; D045542:PQQ Cofactor; D005948:Glucose Dehydrogenases; D042843:Glucose 1-Dehydrogenase; D005947:Glucose