Increased platelet-activating factor (PAF) concentrations in hearts and lungs of Pichinde virus-infected guinea pigs.
Platelet-activating factor (PAF) has been implicated as a cause of cardiopulmonary disturbances in certain diseases. In the present study, concentrations of PAF in hearts, lungs, whole blood, and other organs of control and Pichinde virus-infected guinea pigs on post-inoculation days (PID) 10 and 14 were measured by radioimmunoassay. Results were further confirmed by bioassay after separation and purification with thin-layer chromatography. PAF concentration in the hearts and lungs of virus-infected animals increased significantly on PID 10 and 14, as compared with control levels. PAF level in the blood of infected guinea pigs also significantly increased on PID 14. There was little change of PAF levels in liver and kidney after viral inoculation. Increased PAF concentrations in both hearts and lungs of infected strain 13 guinea pigs suggest that this lipid mediator may play an important role in the development of cardiopulmonary disturbances.
Qian
C
C
Department of Clinical and Experimental Physiology, United States Army Medical Research Institute of Infectious Disease, Fort Detrick, Frederick, MD 21702-5011.
Liu
C T
CT
Peters
C J
CJ
D000818:Animals; D001117:Arenaviridae Infections / Q000097:blood / Q000378:metabolism*; D001681:Biological Assay; D006168:Guinea Pigs; D007668:Kidney / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008168:Lung / Q000378:metabolism*; D008297:Male; D009206:Myocardium / Q000378:metabolism*; D009928:Organ Specificity / Q000502:physiology; D010972:Platelet Activating Factor / Q000302:isolation & purification / Q000378:metabolism*; D011817:Rabbits; D011863:Radioimmunoassay
D010972:Platelet Activating Factor