Viral inactivation in blood and red cell concentrates with benzoporphyrin derivative.
Using both the vesicular stomatitis virus (VSV) and feline leukemia virus (FeLV) as models we have shown that the photosensitizer benzoporphyrin derivative ring A (BPD), when activated with red light (600-700 nm), is effective in eliminating both free virus and virally infected cells from spiked blood products and whole blood drawn from viremic cats experimentally infected with FeLV, under conditions which appear to share red blood cells. The effect of photodynamic therapy on infected lymphocytes, as visualized by scanning electron microscopy, initially appeared as a limited area of tiny holes in the membrane. These holes were subsequently seen to increase in size until the membrane appeared completely decomposed. The red cell membranes however, seem to be undamaged by such photodynamic treatment.
North
J
J
Quadra Logic Technologies Inc., Vancouver, British Columbia, Canada.
Neyndorff
H
H
King
D
D
Levy
J G
JG
D000818:Animals; D001769:Blood / Q000187:drug effects / Q000382:microbiology* / Q000528:radiation effects; D002415:Cats / Q000097:blood / Q000382:microbiology; D016923:Cell Death; D004912:Erythrocytes / Q000187:drug effects / Q000382:microbiology* / Q000528:radiation effects; D006801:Humans; D005256:Leukemia Virus, Feline / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects; D016582:Leukemia, Feline / Q000097:blood / Q000382:microbiology; D008855:Microscopy, Electron, Scanning; D008958:Models, Molecular; D010777:Photochemistry; D011166:Porphyrins / Q000494:pharmacology* / Q000528:radiation effects; D011838:Radiation-Sensitizing Agents / Q000494:pharmacology* / Q000528:radiation effects; D013601:T-Lymphocytes / Q000187:drug effects / Q000382:microbiology / Q000528:radiation effects / Q000648:ultrastructure; D014721:Vesicular stomatitis Indiana virus / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects; D014766:Viremia / Q000097:blood / Q000382:microbiology; D018406:Virus Physiological Phenomena; D014779:Virus Replication / Q000187:drug effects / Q000528:radiation effects; D014780:Viruses / Q000187:drug effects* / Q000528:radiation effects
D011166:Porphyrins; D011838:Radiation-Sensitizing Agents; C504224:benzoporphyrin D