title: A biochemical and NMR spectroscopic study of hydrazine in the isolated rat hepatocyte.
abstract: Using isolated rat hepatocytes the biochemical effects of hydrazine have been investigated using both conventional assay techniques and high resolution proton NMR. High resolution proton NMR revealed that hydrazine caused a significant increase in alanine and lactate levels in the incubation buffer, whereas levels of beta-hydroxybutyrate were decreased. NMR also detected metabolites of hydrazine notably acetylhydrazine and a cyclised hydrazone formed with alpha-ketoglutarate. Changes were detected in NADH and NADPH, ATP, succinate dehydrogenase (SDH) and total non-protein sulphydryl groups (TNPSH). However, the changes in pyridine nucleotides occurred at higher concentrations than those affecting succinate dehydrogenase and ATP. Similarly, the depletion of TNPSH occurred at a higher concentration and with a different time course to that seen with ATP depletion and inhibition of succinate dehydrogenase.
authors.affiliation: Department of Toxicology, London School of Pharmacy, UK.
authors.forename: S
authors.initials: S
authors.lastname: Ghatineh
authors.forename: W
authors.initials: W
authors.lastname: Morgan
authors.forename: N E
authors.initials: NE
authors.lastname: Preece
authors.forename: J A
authors.initials: JA
authors.lastname: Timbrell
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001711:Biotransformation; D005978:Glutathione / Q000378:metabolism; D006834:Hydrazines / Q000032:analysis / Q000378:metabolism* / Q000633:toxicity; D066298:In Vitro Techniques; D008099:Liver / Q000737:chemistry / Q000166:cytology / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D009243:NAD / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D009711:Nucleotides / Q000378:metabolism; D010084:Oxidation-Reduction; D011522:Protons; D051381:Rats; D017207:Rats, Sprague-Dawley; D013385:Succinate Dehydrogenase / Q000378:metabolism; D013438:Sulfhydryl Compounds / Q000378:metabolism
chemical_list: D006834:Hydrazines; D009711:Nucleotides; D011522:Protons; D013438:Sulfhydryl Compounds; D009243:NAD; C029424:hydrazine; D009249:NADP; D000255:Adenosine Triphosphate; D013385:Succinate Dehydrogenase; D005978:Glutathione