Presence of lower temperature-dependent antibody with low avidity to C100-3 (HCV) antigen in voluntary blood donors.
Serum samples with lower temperature-dependent antibody with low avidity to C100-3 (HCV) antigen were found in 0.19% of 23,197 voluntary blood donors at this blood center. They showed positive C100-3 antibody activity at 24 C but not at 37 C. The antibody activity bound to C100-3 antigen at lower temperature disappeared after incubation for 60 min at 37 C or treatment with 8 M urea. Other markers of hepatitis C virus infection, especially the presence of HCV-RNA were demonstrated in some of these serum samples and the importance of this phenomenon is discussed with regard to virus screening of blood donors for hepatitis C.
Yamaki
M
M
Japanese Red Cross Central Blood Center, Tokyo.
Shimada
T
T
Matsumoto
C
C
Watanabe
J
J
Nishioka
K
K
D000915:Antibody Affinity / Q000502:physiology*; D000956:Antigens, Viral; D001782:Blood Donors; D003080:Cold Temperature; D004492:Edetic Acid; D016174:Hepacivirus / Q000276:immunology*; D006508:Hepatitis Antibodies / Q000032:analysis* / Q000276:immunology; D006801:Humans; D017361:Viral Nonstructural Proteins; D014764:Viral Proteins / Q000276:immunology*
D000956:Antigens, Viral; C064544:C100 protein, hepatitis C virus; D006508:Hepatitis Antibodies; D017361:Viral Nonstructural Proteins; D014764:Viral Proteins; D004492:Edetic Acid