High correlation in antibody titers between the Sabin-Feldman dye test and an enzyme-linked immunosorbent assay detecting immunoglobulin G antibodies to the nucleoside triphosphate hydrolase of Toxoplasma gondii.
An enzyme-linked immunosorbent assay to detect immunoglobulin G antibodies against nucleoside triphosphate hydrolase, which is a specific and dominant antigen of Toxoplasma gondii, was developed, and the sensitivity and specificity of the test were compared with those of the Sabin-Feldman dye test. One hundred percent agreement was observed in comparative study between those tests on 37 positive and 50 negative human sera. Antibody titers in the enzyme-linked immunosorbent assay test, which were expressed as the reciprocal of the highest positive dilution of serum, were just 100 times those in the dye test on 81% (30 of 37) of the positive sera.
Asai
T
T
Department of Microbiology, Tokyo Medical College, Japan.
Mizuno
F
F
Kojima
S
S
Takeuchi
T
T
Kobayashi
A
A
Suzuki
Y
Y
D000818:Animals; D000913:Antibodies, Protozoan / Q000032:analysis*; D004396:Coloring Agents; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis*; D043583:Nucleoside-Triphosphatase; D010744:Phosphoric Monoester Hydrolases / Q000276:immunology*; D014122:Toxoplasma / Q000201:enzymology / Q000276:immunology*
D000913:Antibodies, Protozoan; D004396:Coloring Agents; D007074:Immunoglobulin G; D010744:Phosphoric Monoester Hydrolases; D043583:Nucleoside-Triphosphatase