The effect of n-6 fatty acids on normal and v-Ki ras transformed NIH-3T3 cells.
The effect of exogenous gamma-linolenic acid (18:3n-6) was examined on NIH-3T3 and a subclone expressing the v-Ki-ras oncogene (DT). 18:3n-6 inhibited DT cell growth more readily than NIH-3T3 cell growth. In comparison, linoleic acid (18:2n-6) had no effect on the growth of either cell line. DT cells elongated and desaturated both 18:2n-6 and 18:3n-6 to dihomo-gamma-linolenic acid (20:3n-6) and arachidonic acid (20:4n-6) to a much greater extent than NIH-3T3 cells and had a much higher membrane fluidity. The presence of the ras gene or its product appears to increase the metabolism of polyunsaturated fatty acids and potentiate the cytostatic actions of 18:3n-6.
Cantrill
R C
RC
EFAMOL Research Institute, Kentville, Nova Scotia, Canada.
Ells
G W
GW
de Antueno
R J
RJ
Elliot
M
M
Raha
S K
SK
Horrobin
D F
DF
D016475:3T3 Cells; D000818:Animals; D002455:Cell Division / Q000187:drug effects*; D002461:Cell Line, Transformed; D002471:Cell Transformation, Neoplastic; D002999:Clone Cells; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology*; D005454:Fluorescence Polarization; D011905:Genes, ras; D008042:Linolenic Acids / Q000494:pharmacology*; D008560:Membrane Fluidity / Q000187:drug effects; D051379:Mice; D015689:Oncogene Protein p21(ras) / Q000235:genetics; D013329:Structure-Activity Relationship; D017965:gamma-Linolenic Acid
D005231:Fatty Acids, Unsaturated; D008042:Linolenic Acids; D017965:gamma-Linolenic Acid; D015689:Oncogene Protein p21(ras)