Ultrastructural changes associated with reduced mitochondrial DNA and impaired mitochondrial function in the presence of 2'3'-dideoxycytidine.
Incubation of Molt-4 cells in 4 microM 2'3'-dideoxycytidine did not produce a significant change in the mitochondrial ultrastructure after 4 days; however, by 12 days, the mitochondrial ultrastructure was distorted, with condensed cristae or vacuolization, or both. Concentration-dependent decreases in both cell growth (mean 50% inhibitory concentration, 4.70 +/- 0.5 microM) and mitochondrial DNA content (mean 50% inhibitory concentration, 0.46 +/- 0.06 microM) occurred after incubation with 2'3'-dideoxycytidine for 4 days.
Lewis
L D
LD
Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
Hamzeh
F M
FM
Lietman
P S
PS
D002478:Cells, Cultured; D004272:DNA, Mitochondrial / Q000378:metabolism*; D007773:Lactates / Q000378:metabolism; D004915:Leukemia, Erythroblastic, Acute / Q000378:metabolism; D008854:Microscopy, Electron; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure*; D014407:Tumor Cells, Cultured; D016047:Zalcitabine / Q000494:pharmacology*
D004272:DNA, Mitochondrial; D007773:Lactates; D016047:Zalcitabine