Effect of perturbing diacylglycerol metabolism on cytosolic free Ca2+ oscillations induced in single hepatocytes.
Single rat hepatocytes microinjected with aequorin show free Ca oscillations when stimulated with Ca(2+)-mobilizing hormones. We show here that an inhibitor of diacylglycerol kinase (R 59 022) and an analogue of native diacylglycerol (diC8) inhibit free Ca oscillations induced by phenylephrine and vasopressin. These results agree with a negative feedback effect of protein kinase C on free Ca oscillations.
Sanchez-Bueno
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A
Department of Human Anatomy and Cell Biology, University of Liverpool, UK.
Cobbold
P H
PH
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D003600:Cytosol / Q000378:metabolism; D019852:Diacylglycerol Kinase; D004075:Diglycerides / Q000378:metabolism*; D007700:Kinetics; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D009991:Oscillometry; D010656:Phenylephrine / Q000494:pharmacology*; D010770:Phosphotransferases / Q000037:antagonists & inhibitors; D011744:Pyrimidinones / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013844:Thiazoles / Q000494:pharmacology*
D004075:Diglycerides; D011744:Pyrimidinones; D013844:Thiazoles; D010656:Phenylephrine; C047505:R 59022; D010770:Phosphotransferases; D019852:Diacylglycerol Kinase; D002118:Calcium