Photochrome-labeling method in study of dynamics of biological systems.
The theoretical considerations and experimental evidences discussed in this paper indicate that quantitative study of photochromic processes in labeled objects open up new possibilities for investigating microviscosity and conformation transitions in biological systems. The proposed method of photochrome labeling features higher sensitivity and simplicity, and uses labels which are more stable under physiological conditions compared with traditional spin labels.
Likhtenshtein
G I
GI
Institute of Chemical Physics, USSR Academy of Science Chernogolovka.
Khudjakov
D V
DV
Vogel
V R
VR
D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid; D003497:Cyclic N-Oxides; D004578:Electron Spin Resonance Spectroscopy; D005453:Fluorescence; D007700:Kinetics; D008954:Models, Biological; D009211:Myoglobin / Q000032:analysis; D009569:Nitric Oxide; D010777:Photochemistry / Q000379:methods*; D011506:Proteins / Q000032:analysis*; D013113:Spin Labels; D014783:Viscosity
D003497:Cyclic N-Oxides; D009211:Myoglobin; D011506:Proteins; D013113:Spin Labels; C003028:N-(1-oxyl-2,2,6,6-tetramethyl-4-piperidinyl)iodoacetamide; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid; D009569:Nitric Oxide