title: A variant serotype G3 rotavirus isolated from an unusually severe outbreak of diarrhoea in piglets.
abstract: About 80% of faecal samples from severe outbreak of porcine diarrhoea (scours) were positive for rotavirus. Rotavirus positive samples were analyzed for their antigenic properties and amino acid sequences of the glycoprotein genes. These viruses could not be assigned to any serotypes using serotyping monoclonal antibodies (MAbs) developed for porcine rotaviruses [Nagesha and Holmes: Journal of Medical Virology 35:206-211, 1991b]. When two such viruses were isolated in cell culture and analyzed by neutralization tests using hyperimmune sera they showed only one way antigenic relation with both human and porcine viruses belonging to serotype G3. In addition none of the serotyping MAbs neutralized these two virus isolates. There was no base variation between VP7 genes of faecal and cell culture isolates. Predicted amino acid sequences of the VP7 gene showed marked epitope variation from other porcine type G3 isolates with amino acid substitutions and an additional glycosylation site at residue 238. This antigenic variation seen in rotaviruses appears similar to that of influenza viruses undergoing antigenic drift.
authors.affiliation: School of Microbiology, University of Melbourne, Parkville, Victoria, Australia.
authors.forename: H S
authors.initials: HS
authors.lastname: Nagesha
authors.forename: J
authors.initials: J
authors.lastname: Huang
authors.forename: I H
authors.initials: IH
authors.lastname: Holmes
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000940:Antigenic Variation / Q000235:genetics; D000956:Antigens, Viral; D001315:Australia / Q000453:epidemiology; D002213:Capsid / Q000235:genetics / Q000276:immunology; D036022:Capsid Proteins; D003967:Diarrhea / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D004196:Disease Outbreaks / Q000662:veterinary*; D014644:Genetic Variation; D008969:Molecular Sequence Data; D012401:Rotavirus / Q000145:classification* / Q000235:genetics / Q000276:immunology; D012400:Rotavirus Infections / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D017386:Sequence Homology, Amino Acid; D012703:Serotyping; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology / Q000382:microbiology*
chemical_list: D000956:Antigens, Viral; D036022:Capsid Proteins; C050779:VP7 protein, Rotavirus