Description of an in situ hybridization methodology for detection of Epstein-Barr virus RNA in paraffin-embedded tissues, with a survey of normal and neoplastic tissues.
The authors describe a highly sensitive and practical in situ hybridization method using an oligonucleotide probe for EBER1 RNA for the detection of Epstein-Barr virus (EBV) in formalin-fixed, paraffin-embedded tissue sections. Paraffin-embedded tissues from 793 cases of normal and neoplastic tissues were studied. Nuclear staining for EBV RNA was uniformly present in all or virtually all neoplastic cells in a variety of known EBV-positive tumors. We also demonstrate rare EBV-infected cells in normal lymphoid tissues. RNAase predigestion, competitive inhibition, and control probe studies confirmed the specificity of the staining. In addition, cross-reactivity of EBV RNA staining with other viruses was not present. Additionally, the distribution of EBV in a wide variety of other normal and neoplastic tissues is reported.
Chang
K L
KL
Department of Pathology, City of Hope National Medical Center, Duarte, California.
Chen
Y Y
YY
Shibata
D
D
Weiss
L M
LM
D001483:Base Sequence; D004279:DNA, Viral / Q000235:genetics; D005069:Evaluation Studies as Topic; D005260:Female; D005814:Genes, Viral; D006566:Herpesviridae Infections / Q000175:diagnosis / Q000382:microbiology; D004854:Herpesvirus 4, Human / Q000235:genetics* / Q000302:isolation & purification*; D006801:Humans; D017403:In Situ Hybridization / Q000379:methods* / Q000706:statistics & numerical data; D008297:Male; D008969:Molecular Sequence Data; D009369:Neoplasms / Q000382:microbiology / Q000473:pathology; D015345:Oligonucleotide Probes; D016612:Paraffin Embedding; D012367:RNA, Viral / Q000235:genetics* / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D014412:Tumor Virus Infections / Q000175:diagnosis / Q000382:microbiology
D004279:DNA, Viral; D015345:Oligonucleotide Probes; D012367:RNA, Viral