High levels of reactive oxygen metabolites in colon cancer tissue: analysis by chemiluminescence probe.
Reactive oxygen metabolites (ROM) have been postulated to contribute to the development of various carcinomas, including colon cancer. Indeed, the effects of ROM scavengers are being tested for chemoprevention of adenocarcinoma of the colon. However, there has been no evidence to indicate that high levels of ROM are indeed present in cancerous tissue. In this study, we used a chemiluminescence probe to estimate ROM levels in cancerous and neighboring noncancerous colonic tissues from seven patients with colon cancer. Cancerous tissues contained significantly (p less than 0.05) more luminol-enhanced chemiluminescence (4,808 +/- 2,282 counts/min/mg protein) than neighboring noncancerous tissues (2,175 +/- 1,111). The addition of an ROM scavenger, catalase (2, 4, and 8 micrograms/ml), to the tissue suspension inhibited chemiluminescence produced by both noncancerous (-74%, -85%, and -71%) and cancerous (-11%, -61%, and -53%) tissues. This study shows that colonic cancerous tissue contains high levels of ROM, which may play an important role in the pathogenesis of colon cancer.
Keshavarzian
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A
Department of Medicine, Loyola University Medical School, Maywood, IL 60153.
Zapeda
D
D
List
T
T
Mobarhan
S
S
D000230:Adenocarcinoma / Q000378:metabolism; D000368:Aged; D000369:Aged, 80 and over; D003110:Colonic Neoplasms / Q000378:metabolism*; D006801:Humans; D007958:Leukocyte Count; D008163:Luminescent Measurements; D008165:Luminol; D008297:Male; D008875:Middle Aged; D009195:Peroxidase / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism*; D012016:Reference Values
D017382:Reactive Oxygen Species; D008165:Luminol; D009195:Peroxidase