Activation-induced ubiquitination of the T cell antigen receptor.
The zeta subunit of the T cell antigen receptor (TCR) exists primarily as a disulfide-linked homodimer. This receptor subunit is important in TCR-mediated signal transduction and is a substrate for a TCR-activated protein tyrosine kinase. The zeta chain was found to undergo ubiquitination in response to receptor engagement. This posttranslational modification occurred in normal T cells and tumor lines. Both nonphosphorylated and phosphorylated zeta molecules were modified, and at least one other TCR subunit, CD3 delta, was also ubiquitinated after activation of the receptor. These findings suggest an expanded role for ubiquitination in transmembrane receptor function.
Cenciarelli
C
C
Experimental Immunology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892.
Hou
D
D
Hsu
K C
KC
Rellahan
B L
BL
Wiest
D L
DL
Smith
H T
HT
Fried
V A
VA
Weissman
A M
AM
D000818:Animals; D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology / Q000378:metabolism; D017252:CD3 Complex; D002478:Cells, Cultured; D006825:Hybridomas / Q000276:immunology; D008213:Lymphocyte Activation / Q000502:physiology*; D046911:Macromolecular Substances; D051379:Mice; D008810:Mice, Inbred C57BL; D008970:Molecular Weight; D011499:Protein Processing, Post-Translational; D011948:Receptors, Antigen, T-Cell / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D014452:Ubiquitins / Q000302:isolation & purification / Q000378:metabolism*
D000945:Antigens, Differentiation, T-Lymphocyte; D017252:CD3 Complex; D046911:Macromolecular Substances; D011948:Receptors, Antigen, T-Cell; D014452:Ubiquitins