Time course of sodium-induced Na(+)-K(+)-ATPase recruitment in rabbit cortical collecting tubule.
In cortical collecting tubules (CCD) of aldosterone-repleted rabbit kidney, an increase in intracellular sodium concentration (Nai) induces the recruitment and/or activation of latent Na(+)-K(+)-ATPase pumps (Blot-Chabaud et al., J. Biol. Chem. 265: 11676-11681, 1990). The present study was addressed to determine the time course of this Nai-dependent pump recruitment and to examine some of the factors possibly involved in this phenomenon. CCD from adrenalectomized rabbits complemented with aldosterone and dexamethasone were incubated at 4 degrees C either in a K(+)-free saline solution (Na(+)-loaded CCD) or in a sucrose solution (control CCD) and then rewarmed for various time periods to allow pump recruitment to occur. The number of pumps in the membrane was determined by specific [3H]ouabain binding; Nai was measured using 22Na. A rise in Nai induced a threefold increase in the number of basolateral pumps, which was fully achieved within 1-2 min. This pump recruitment was reversible within 15 min after restoration of low Nai. It was unaffected by inhibitors of cytoskeleton and Ca2+ ionophore A 23187. The blocker of the Na(+)-H+ antiporter, amiloride, did not prevent it. The protein kinase C activator, phorbol 12-myristate 13-acetate, did not induce it in the absence of Na+. We conclude that Nai is a major determinant of pump recruitment and/or activation, which occurs over a very short period of time. It may constitute a rapid adaptative response to an increase in the cell Na+ load.
Coutry
N
N
Institut National de la Santé et de la Recherche Médicale Unité 246, Paris, France.
Blot-Chabaud
M
M
Mateo
P
P
Bonvalet
J P
JP
Farman
N
N
D000818:Animals; D004790:Enzyme Induction; D005260:Female; D007672:Kidney Cortex; D007685:Kidney Tubules, Collecting / Q000378:metabolism*; D010042:Ouabain / Q000378:metabolism; D011817:Rabbits; D012964:Sodium / Q000494:pharmacology*; D000254:Sodium-Potassium-Exchanging ATPase / Q000187:drug effects / Q000378:metabolism*; D013696:Temperature; D013997:Time Factors; D014638:Vanadates / Q000494:pharmacology
D014638:Vanadates; D010042:Ouabain; D012964:Sodium; D000254:Sodium-Potassium-Exchanging ATPase