Amplification of the c-myc proto-oncogene in human chondrosarcoma.
The genomic organization of four oncogenes, i.e., c-myc, c-myb, c-Ha-ras, and c-fms, was investigated in fresh surgical specimens from 10 patients with cartilaginous tumors. Among nine chondrosarcomas, six were primary lesions and three local recurrences. The remaining case was a chondroblastoma. Amplification of the c-myc proto-oncogene was the sole abnormality detected in this series, occurring in two chondrosarcomas (four- and eight-fold). No other genetic alteration such as oncogene rearrangement was found. Nor was there any amplification of the other oncogenes studied. Both c-myc-amplified tumors were primary lesions and histologically classified as grade II; according to flow DNA cytometry, one was diploid and the other aneuploid. In our limited series, there was no overall relationship between c-myc amplification, on the one hand, and histologic subtype, malignancy grade, surgical stage, or ploidy level, on the other. Our study shows that amplification of the c-myc oncogene, presumed to be involved in the development of malignancy, is encountered in occasional human chondrosarcomas, however, without any relationship to other well-known features of this tumor entity. The clinical significance of this gene amplification remains to be established.
Castresana
J S
JS
Department of Tumor Pathology, Karolinska Hospital, Stockholm, Sweden.
Barrios
C
C
Gómez
L
L
Kreicbergs
A
A
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000782:Aneuploidy; D015139:Blotting, Southern; D001859:Bone Neoplasms / Q000235:genetics*; D002804:Chondroblastoma / Q000235:genetics; D002813:Chondrosarcoma / Q000235:genetics*; D018211:Chondrosarcoma, Mesenchymal / Q000235:genetics; D015342:DNA Probes; D004171:Diploidy; D005260:Female; D005784:Gene Amplification; D016236:Genes, fms; D016259:Genes, myc; D011905:Genes, ras; D006801:Humans; D008297:Male; D008875:Middle Aged; D009857:Oncogenes; D011379:Prognosis; D000090063:Proto-Oncogene Mas
D015342:DNA Probes; C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas