Assignment of the redox potentials to the four haems in Desulfovibrio vulgaris cytochrome c3 by 2D-NMR.
Using 2D-NMR the four haems of Desulfovibrio vulgaris (Hildenborough) cytochrome c3 within the X-ray structure were fully cross assigned according to their redox potential. The strategy used was based on a complete network of chemical exchange connectivities between the NMR signals obtained for all oxidation levels to the corresponding ones in the fully reduced spectrum [1992, Eur. J. Biochem., in press]. This unequivocal cross-assignment disagrees with earlier results obtained for the similar protein from Desulfovibrio vulgaris (Miyazaki F.).
Salgueiro
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CA
Centro de Tecnologia Química e Biológica, Oeiras, Portugal.
Turner
D L
DL
Santos
H
H
LeGall
J
J
Xavier
A V
AV
D003574:Cytochrome c Group / Q000737:chemistry*; D016969:Desulfovibrio vulgaris / Q000737:chemistry*; D006418:Heme / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D010084:Oxidation-Reduction; D013329:Structure-Activity Relationship; D014961:X-Ray Diffraction
D003574:Cytochrome c Group; D006418:Heme; C017165:cytochrome c(3)