Transformation of cauliflower (Brassica oleracea L. var. botrytis)--an experimental survey.
The paper compares different approaches for the genetic transformation of cauliflower (Agrobacterium-mediated, PEG-mediated and/or electroporation). Transient expression of the neomycin phosphotransferase II (NPTII) gene could be detected after direct gene transfer. Stable transformation was achieved using both Agrobacterium-mediated and direct gene transfer. Expression as well as incorporation of the NPTII sequence could be demonstrated.
Eimert
K
K
Dept. of Genetics, Martin Luther University, Halle/S., Germany.
Siegemund
F
F
D001937:Brassica / Q000235:genetics*; D019868:Kanamycin Kinase; D010770:Phosphotransferases / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D010957:Plasmids; D011523:Protoplasts / Q000201:enzymology; D012231:Rhizobium / Q000235:genetics*; D014162:Transfection; D014170:Transformation, Genetic
D010770:Phosphotransferases; D019868:Kanamycin Kinase