Further diagnostic use of an invasive-specific monoclonal antibody against Entamoeba histolytica.
Genotypic differences between invasive and non-invasive E. histolytica could explain the 1:10 ratio of symptomatic/asymptomatic infection worldwide. Currently, zymodeme analysis is used to differentiate invasive from non-invasive E. histolytica strains but the technique is cumbersome and expensive. In accordance with the WHO research priorities for amebiasis we report here the further use of an invasive-specific monoclonal antibody against E. histolytica in immunofluorescence, to identify isolates cultured from stool samples of patients from three geographically distant endemic regions: Bangladesh, Colombia and Mexico. We tested 107 E. histolytica isolates and the correlation between zymodeme characterization and the immunofluorescence assay was 100%.
González-Ruíz
A
A
Department of Medical Parasitology, London School of Hygiene and Tropical Medicine, UK.
Haque
R
R
Rehman
T
T
Aguirre
A
A
Castañón
G
G
Hall
A
A
Guhl
F
F
Ruíz-Palacios
G
G
Warhurst
D C
DC
Miles
M A
MA
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000913:Antibodies, Protozoan / Q000276:immunology*; D001459:Bangladesh / Q000453:epidemiology; D003105:Colombia / Q000453:epidemiology; D004404:Dysentery, Amebic / Q000453:epidemiology / Q000469:parasitology*; D004748:Entamoeba histolytica / Q000201:enzymology / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification / Q000472:pathogenicity; D005243:Feces / Q000469:parasitology; D005455:Fluorescent Antibody Technique; D007527:Isoenzymes / Q000032:analysis; D008800:Mexico / Q000453:epidemiology; D015800:Protozoan Proteins / Q000032:analysis; D014774:Virulence
D000911:Antibodies, Monoclonal; D000913:Antibodies, Protozoan; D007527:Isoenzymes; D015800:Protozoan Proteins