title: Epidermal and splenic antigen-presenting cell function in a retrovirally induced murine immunodeficiency syndrome (MAIDS).
abstract: Since alterations of epidermal Langerhans cells (LC) have been observed in humans infected with HIV, we investigated the morphology and function of these cells in murine acquired immunodeficiency syndrome (MAIDS), a murine model closely resembling human AIDS. The number as well as the shape of dendritic MHC class II+ cells from ear skin of C57BL/6 mice were similar in normal and infected animals. In mixed epidermal cell (EC) lymphocyte cultures, EC from infected mice and from normal mice stimulated allogeneic T cell proliferation to the same extent. In contrast to T cells from normal mice, however, T cells from infected mice did not respond to allogeneic spleen cells, confirming the presence of a T-cell defect in MAIDS. Subcutaneous injection of syngeneic mice with trinitrophenyl-modified MAIDS EC resulted in delayed ear swelling responses after challenge that were equivalent to those induced by hapten-modified EC from normal mice, suggesting that the contact sensitivity inducing potential of MAIDS LC was preserved. To investigate antigen presenting and processing function, EC and spleen cells were tested with the ovalbumin-specific IAb-restricted T cell hybridoma BO.17.10 and either ovalbumin 323-339 peptide or intact ovalbumin protein. MAIDS spleen cells had a reduced antigen presenting capacity compared with normal spleen cells, whereas EC from these mice showed the same processing and presenting capacity as normal controls. In summary, our results demonstrate that the frequency, morphology, level of MHC class II antigen expression and ability to process and present antigen is normal for LC from mice with MAIDS whereas the function of splenic T cells and APC from infected mice is significantly impaired.
authors.affiliation: Department of Medicine, Hôpital Cantonal Universitaire, Geneva, Switzerland.
authors.forename: A
authors.initials: A
authors.lastname: Cerny
authors.forename: S
authors.initials: S
authors.lastname: Izui
authors.forename: J H
authors.initials: JH
authors.lastname: Saurat
authors.forename: F A
authors.initials: FA
authors.lastname: Waldvogel
authors.forename: H C
authors.initials: HC
authors.lastname: Morse
authors.forename: C
authors.initials: C
authors.lastname: Hauser
mesh_terms: D000818:Animals; D000938:Antigen-Presenting Cells / Q000502:physiology*; D003877:Dermatitis, Contact / Q000209:etiology; D004817:Epidermis / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D009052:Leukemia Virus, Murine; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D016183:Murine Acquired Immunodeficiency Syndrome / Q000276:immunology*; D013154:Spleen / Q000276:immunology*