title: Immunohistochemical characterization of an amphicrine mucinous islet-cell carcinoma of the pancreas. Case report.
abstract: Immunohistochemical characteristics of a mucinous islet-cell carcinoma of the pancreas are described. The tumour presented with jaundice in a 59-year-old male. It consisted of polygonal atypical cells forming a reticular pattern, and invaded the common bile duct. In DNA flow cytometry, the tumour cells showed a clear-cut aneuploid peak. Intercellular mucin was abundant. A panel of antisera and monoclonal markers was applied in the immunohistochemical analysis. In addition to general epithelial and endocrine markers, the tumour cells showed a focal positive immunoreaction with anti-glucagon, anti-insulin, anti-vasoactive intestinal polypeptide, anti-pancreatic secretory trypsin inhibitor and anti-phospholipase A2 antigen. At the ultrastructural level, mucous and neuroendocrine granules were demonstrated in the same tumour cells.
authors.affiliation: Department of Pathology, University of Turku, Finland.
authors.forename: V J
authors.initials: VJ
authors.lastname: Laine
authors.forename: T O
authors.initials: TO
authors.lastname: Ekfors
authors.forename: R
authors.initials: R
authors.lastname: Gullichsen
authors.forename: T J
authors.initials: TJ
authors.lastname: Nevalainen
mesh_terms: D007516:Adenoma, Islet Cell / Q000276:immunology / Q000378:metabolism / Q000473:pathology*; D002277:Carcinoma / Q000276:immunology / Q000378:metabolism / Q000473:pathology*; D003594:Cytoplasmic Granules / Q000648:ultrastructure; D004273:DNA, Neoplasm / Q000032:analysis; D005934:Glucagon / Q000378:metabolism; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D008562:Membrane Glycoproteins / Q000378:metabolism; D008875:Middle Aged; D018396:Mucin-1; D009077:Mucins / Q000378:metabolism; D009418:S100 Proteins / Q000378:metabolism; D014361:Trypsin Inhibitors / Q000378:metabolism
chemical_list: D004273:DNA, Neoplasm; D008562:Membrane Glycoproteins; D018396:Mucin-1; D009077:Mucins; D009418:S100 Proteins; D014361:Trypsin Inhibitors; D005934:Glucagon