title: Energetic metabolism of glucose, mannose and galactose in glucose-starved rat insulinoma cells anchored on microcarrier beads. A phosphorus-31 NMR study.
abstract: Insulin-secreting cells (RINm5F) have successfully been grown on a large scale on poly-L-lysine coated-polystyrene microcarriers, providing a high cell number in a restricted volume under conditions that respect the metabolic integrity of these anchorage-dependent cells. The energetic metabolism of the perfused cells has been followed non-invasively by phosphorus-31 nuclear magnetic resonance spectroscopy. Glucose starvation induced a rapid decrease in nucleoside triphosphates (mainly ATP) pools, correlated with an increase in Pi level. The initial ATP level was rapidly recovered when the cells were refed with glucose or with mannose, but not with galactose, even after 2 h of perfusion. These differential effects of hexoses on energetic metabolism might be related to their various insulin-release actions on tumor islet cells.
authors.affiliation: Centre de Résonance Magnétique Biologique et Médicale (CRMBM), URA CNRS 1186, Faculté de Médecine, Marseille, France.
authors.forename: M
authors.initials: M
authors.lastname: Kriat
authors.forename: J
authors.initials: J
authors.lastname: Fantini
authors.forename: J
authors.initials: J
authors.lastname: Vion-Dury
authors.forename: S
authors.initials: S
authors.lastname: Confort-Gouny
authors.forename: J P
authors.initials: JP
authors.lastname: Galons
authors.forename: P J
authors.initials: PJ
authors.lastname: Cozzone
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002457:Cell Extracts / Q000737:chemistry; D004734:Energy Metabolism / Q000502:physiology*; D005690:Galactose / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D007340:Insulinoma / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008358:Mannose / Q000378:metabolism*; D008863:Microspheres; D010472:Perchlorates; D010477:Perfusion; D010758:Phosphorus / Q000378:metabolism; D051381:Rats; D013217:Starvation / Q000378:metabolism; D014407:Tumor Cells, Cultured
chemical_list: D002457:Cell Extracts; D010472:Perchlorates; D010758:Phosphorus; D000255:Adenosine Triphosphate; D005947:Glucose; D008358:Mannose; D005690:Galactose