Inhibitory effects of crude soybean trypsin inhibitor on pancreatic ductal carcinogenesis in hamsters after initiation with N-nitrosobis(2-oxopropyl)amine.
The effects of soybean trypsin inhibitor (SBTI) administration during the promotion phase of pancreatic carcinogenesis were investigated. Female Syrian golden hamsters were given three weekly s.c. injections of N-nitrosobis(2-oxopropyl)amine (BOP) each at a dose of 10 mg/kg and then administered 5% SBTI diet for the following 37 weeks. Additional groups of animals received the BOP injection alone or the 5% SBTI diet alone as controls. At week 40 of the experiment, all surviving animals were killed and development of pancreatic lesions was assessed histopathologically. The results showed that the incidence of dysplastic lesions in hamsters of the BOP/SBTI group was significantly decreased as compared to that of the BOP group (P < 0.01). A similar but not significant tendency was also found for pancreatic adenocarcinomas. In addition, the number of dysplastic lesions in the pancreas head portion in the BOP/SBTI group were significantly decreased as compared to the BOP group value (P < 0.05). Furthermore, atrophic changes of the pancreatic exocrine tissue were more severe in the BOP group than in the BOP/SBTI group (P < 0.01), indicating that SBTI treatment gave effective protection against the replacement process of acinar cell induced by BOP. Thus, the present experiment demonstrated that SBTI can inhibit hamster pancreatic ductal carcinogenesis when given in the promotion phase, in clear contrast to the enhancing effects reported for preneoplastic acinar lesion development in rats.
Furukawa
F
F
Division of Pathology, National Institute of Health Sciences, Tokyo, Japan.
Nishikawa
A
A
Imaida
K
K
Mitsui
M
M
Enami
T
T
Hayashi
Y
Y
Takahashi
M
M
D000818:Animals; D001835:Body Weight / Q000187:drug effects; D002273:Carcinogens / Q000633:toxicity*; D002285:Carcinoma, Intraductal, Noninfiltrating / Q000139:chemically induced*; D006224:Cricetinae; D004347:Drug Interactions; D005260:Female; D008647:Mesocricetus; D009602:Nitrosamines / Q000633:toxicity*; D010190:Pancreatic Neoplasms / Q000139:chemically induced*; D011230:Precancerous Conditions; D014360:Trypsin Inhibitor, Kunitz Soybean / Q000494:pharmacology*
D002273:Carcinogens; D009602:Nitrosamines; C013622:nitrosobis(2-oxopropyl)amine; D014360:Trypsin Inhibitor, Kunitz Soybean