title: Glycinergic ligands modulate the rate of phosphorylation of the glycine receptor by protein kinase C.
abstract: The alpha subunit of the glycine receptor purified from rat spinal cord is rapidly and specifically phosphorylated by protein kinase C (Ruiz-Gómez et al., (1991) J. Biol. Chem. 266, 559-566). We report here that the rate of phosphorylation of the glycine receptor by this kinase is higher in the presence of agonists (glycine, beta-alanine) than in the presence of antagonists (strychnine, RU-5135). These results suggest that activated glycine receptors would be a preferential target for functional regulation through phosphorylation mechanisms.
authors.affiliation: Centro de Biología Molecular Severo Ochoa (CSIC-UAM), Universidad Autònoma, Madrid, Spain.
authors.forename: M L
authors.initials: ML
authors.lastname: Vaello
authors.forename: A
authors.initials: A
authors.lastname: Ruiz-Gómez
authors.forename: F
authors.initials: F
authors.lastname: Mayor
mesh_terms: D000409:Alanine / Q000494:pharmacology*; D000731:Androstanes / Q000494:pharmacology; D000818:Animals; D001378:Azasteroids / Q000494:pharmacology; D001921:Brain / Q000201:enzymology; D005998:Glycine / Q000378:metabolism / Q000494:pharmacology*; D007700:Kinetics; D008024:Ligands; D046911:Macromolecular Substances; D010766:Phosphorylation; D011493:Protein Kinase C / Q000302:isolation & purification / Q000378:metabolism*; D051381:Rats; D018009:Receptors, Glycine; D017981:Receptors, Neurotransmitter / Q000187:drug effects / Q000302:isolation & purification / Q000378:metabolism*; D013116:Spinal Cord / Q000378:metabolism; D013331:Strychnine / Q000378:metabolism / Q000494:pharmacology*
chemical_list: D000731:Androstanes; D001378:Azasteroids; D008024:Ligands; D046911:Macromolecular Substances; D018009:Receptors, Glycine; D017981:Receptors, Neurotransmitter; D011493:Protein Kinase C; D013331:Strychnine; D000409:Alanine; C032061:RU 5135; D005998:Glycine