Stimulation of in vitro steroidogenesis by pituitary hormones in a turtle (Trachemys scripta) within the temperature-sensitive period for sex determination.
To investigate the possible involvement of pituitary hormones in the regulation of steroidogenesis during reptilian sexual differentiation, we tested the ability of gonadotropin (ovine FSH), adrenocorticotropin (porcine ACTH), and growth hormone (bovine GH) to stimulate in vitro steroidogenesis in embryonic adrenal-kidney-gonad complexes (AKGs) of a turtle, Trachemys scripta, during and after the temperature-sensitive period for sex determination (TSP). Radioimmunoassays were used to measure progesterone, testosterone, estradiol, and corticosterone in incubation media; additionally, immunoreactive ACTH was measured in plasma. Presumptive male and female AKGs were stimulated by both FSH and ACTH at each stage investigated. Secretion of progesterone and corticosterone was usually far greater than that of testosterone or estradiol in both basal and hormone-stimulated incubations. In general, AKGs from presumptive males secreted more progesterone and corticosterone than AKGs from presumptive females. Progesterone and estradiol secretions were stimulated by both FSH and ACTH, but testosterone secretion was stimulated only by ACTH. Corticosterone secretion was strongly stimulated by ACTH. GH failed to significantly stimulate steroid secretion. Plasma ACTH levels were significantly higher in males than in females, and both sexes had significantly higher plasma levels of ACTH after the TSP compared to during the TSP. Our data demonstrate that during the temperature-sensitive period AKGs are responsive to both gonadotropin and ACTH, and that there are significant sex differences in steroidogenesis, sensitivity to gonadotropin and ACTH, and plasma ACTH levels.
White
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RB
University of Texas Marine Science Institute, University of Texas at Austin, Port Aransas 78373.
Thomas
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D000324:Adrenocorticotropic Hormone / Q000494:pharmacology; D000704:Analysis of Variance; D000818:Animals; D003345:Corticosterone / Q000096:biosynthesis; D046508:Culture Techniques; D004338:Drug Combinations; D004958:Estradiol / Q000096:biosynthesis; D005260:Female; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D013006:Growth Hormone / Q000494:pharmacology; D006358:Hot Temperature; D008297:Male; D010907:Pituitary Hormones / Q000494:pharmacology*; D011374:Progesterone / Q000096:biosynthesis; D011863:Radioimmunoassay; D012733:Sex Differentiation / Q000502:physiology*; D013256:Steroids / Q000096:biosynthesis*; D013739:Testosterone / Q000096:biosynthesis; D014426:Turtles / Q000196:embryology*
D004338:Drug Combinations; D010907:Pituitary Hormones; D013256:Steroids; D013739:Testosterone; D011374:Progesterone; D004958:Estradiol; D000324:Adrenocorticotropic Hormone; D005640:Follicle Stimulating Hormone; D013006:Growth Hormone; D003345:Corticosterone