title: Assessment of posttraumatic polymorphonuclear leukocyte accumulation in rat brain using tissue myeloperoxidase assay and vinblastine treatment.
abstract: Polymorphonuclear leukocytes (PMN) are implicated in the pathogenesis of traumatic brain injury. We tested the following hypotheses: (1) leukocyte accumulation is present in brain tissue 24 h posttrauma, (2) leukocyte accumulation represents PMN, and (3) prior systemic PMN depletion attenuates brain tissue PMN accumulation. Trauma was induced in exposed right parietal cortex by weightdrop in anesthetized Wistar rats (n = 24). Of the traumatized rats, 12 were PMN-depleted with vinblastine sulfate i.v. Controls were 12 normal rats and 5 sham-operated rats (craniotomy). Sections of traumatized and contralateral hemispheres were analyzed for myeloperoxidase (MPO) activity. Brain MPO activity was increased fivefold at 24 h posttrauma, but only in the traumatized hemisphere (0.448 +/- 0.133 U/g vs 0.090 +/- 0.022 U/g in trauma vs normal, respectively, p < 0.05, mean +/- SEM). PMN depletion attenuated this increase in MPO activity and decreased circulating PMN counts (0.07 +/- 0.032 x 10(9)/L vs 0.894 +/- 0.294 x 10(9)/L PMN-depleted-trauma vs trauma rats, respectively, p < 0.05). Leukocyte accumulation in the brain posttrauma was confirmed by MPO assay. Inhibition of MPO activity in the PMN-depleted group and the specificity of vinblastine treatment for depletion of circulating PMN suggest that leukocyte accumulation in the brain at 24 h posttrauma is largely due to PMN.
authors.affiliation: Department of Anesthesiology and Critical Care Medicine, University of Pittsburgh, Pennsylvania.
authors.forename: K V
authors.initials: KV
authors.lastname: Biagas
authors.forename: M W
authors.initials: MW
authors.lastname: Uhl
authors.forename: J K
authors.initials: JK
authors.lastname: Schiding
authors.forename: E M
authors.initials: EM
authors.lastname: Nemoto
authors.forename: P M
authors.initials: PM
authors.lastname: Kochanek
mesh_terms: D000818:Animals; D001921:Brain / Q000201:enzymology / Q000473:pathology*; D001930:Brain Injuries / Q000201:enzymology / Q000473:pathology*; D007958:Leukocyte Count / Q000187:drug effects; D008214:Lymphocytes / Q000187:drug effects; D008297:Male; D009000:Monocytes / Q000187:drug effects; D009504:Neutrophils / Q000502:physiology*; D009195:Peroxidase / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D014747:Vinblastine / Q000494:pharmacology*
chemical_list: D014747:Vinblastine; D009195:Peroxidase