Signal transduction pathways leading to the production of IL-8 by human monocytes are differentially regulated by dexamethasone.
Previous studies have shown that IL-8 gene expression is enhanced by various stimuli, which induce different signal transduction pathways. A lipopolysaccharide (LPS)-induced pathway has been reported to be inhibited by glucocorticoids in monocytes. We have now examined the effect of dexamethasone on the LPS-induced and other signal transduction pathways leading to the production of IL-8 by human monocytes. Dexamethasone inhibited the production of IL-8 stimulated with a cyclic adenosine monophosphate analog or LPS. In contrast, dexamethasone had no significant effect on a phorbol ester (PMA)-stimulated IL-8 production. These results suggest that the signal transduction pathways leading to the production of IL-8 by human monocytes are differentially regulated by dexamethasone.
Anttila
H S
HS
Department of Dermatology, Helsinki University Central Hospital, Finland.
Reitamo
S
S
Ceska
M
M
Hurme
M
M
D003994:Bucladesine / Q000037:antagonists & inhibitors / Q000494:pharmacology; D003907:Dexamethasone / Q000494:pharmacology*; D006801:Humans; D016209:Interleukin-8 / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000502:physiology; D009000:Monocytes / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D016209:Interleukin-8; D008070:Lipopolysaccharides; D003994:Bucladesine; D003907:Dexamethasone; D013755:Tetradecanoylphorbol Acetate