title: Comparison of antiviral assay methods using cell-free and cell-associated varicella-zoster virus.
abstract: Assay methods for varicella-zoster virus (VZV) susceptibility to acyclovir (ACV) of VZV were compared by using cell-free (CF) and cell-associated (CA) virus of 6 x plaque-purified VZV. The 50% effective doses (ED50) of ACV, as required to reduce virus plaque formation by 50%, were about 8 times higher for CA virus than for CF virus. Also, the ED50 of 1-beta-D-arabinofuranosyl-(E)-5-(2-bromovinyl)uracil (BVaraU) for CA-VZV was higher than for CF-VZV, and fresh clinical isolates of VZV gave higher ACV ED50 values than CF virus. CA virus prepared at various times after CF virus infection showed a gradual increase of the ACV ED50 with time, ranging from the ED50 for CF virus to that for CA virus.
authors.affiliation: Department of Virology, Toyama Medical and Pharmaceutical University, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Shiraki
authors.forename: H
authors.initials: H
authors.lastname: Ochiai
authors.forename: J
authors.initials: J
authors.lastname: Namazue
authors.forename: T
authors.initials: T
authors.lastname: Okuno
authors.forename: S
authors.initials: S
authors.lastname: Ogino
authors.forename: K
authors.initials: K
authors.lastname: Hayashi
authors.forename: K
authors.initials: K
authors.lastname: Yamanishi
authors.forename: M
authors.initials: M
authors.lastname: Takahashi
mesh_terms: D000212:Acyclovir / Q000494:pharmacology; D000998:Antiviral Agents / Q000494:pharmacology*; D001086:Arabinofuranosyluracil / Q000031:analogs & derivatives / Q000494:pharmacology; D002474:Cell-Free System; D004261:DNA Replication / Q000187:drug effects; D004279:DNA, Viral / Q000096:biosynthesis; D014645:Herpesvirus 3, Human / Q000187:drug effects*; D006801:Humans; D009693:Nucleic Acid Hybridization; D010948:Viral Plaque Assay; D014779:Virus Replication / Q000187:drug effects
chemical_list: D000998:Antiviral Agents; D004279:DNA, Viral; D001086:Arabinofuranosyluracil; C031368:sorivudine; D000212:Acyclovir