title: The effect of n-6 fatty acids on normal and v-Ki ras transformed NIH-3T3 cells.
abstract: The effect of exogenous gamma-linolenic acid (18:3n-6) was examined on NIH-3T3 and a subclone expressing the v-Ki-ras oncogene (DT). 18:3n-6 inhibited DT cell growth more readily than NIH-3T3 cell growth. In comparison, linoleic acid (18:2n-6) had no effect on the growth of either cell line. DT cells elongated and desaturated both 18:2n-6 and 18:3n-6 to dihomo-gamma-linolenic acid (20:3n-6) and arachidonic acid (20:4n-6) to a much greater extent than NIH-3T3 cells and had a much higher membrane fluidity. The presence of the ras gene or its product appears to increase the metabolism of polyunsaturated fatty acids and potentiate the cytostatic actions of 18:3n-6.
authors.affiliation: EFAMOL Research Institute, Kentville, Nova Scotia, Canada.
authors.forename: R C
authors.initials: RC
authors.lastname: Cantrill
authors.forename: G W
authors.initials: GW
authors.lastname: Ells
authors.forename: R J
authors.initials: RJ
authors.lastname: de Antueno
authors.forename: M
authors.initials: M
authors.lastname: Elliot
authors.forename: S K
authors.initials: SK
authors.lastname: Raha
authors.forename: D F
authors.initials: DF
authors.lastname: Horrobin
mesh_terms: D016475:3T3 Cells; D000818:Animals; D002455:Cell Division / Q000187:drug effects*; D002461:Cell Line, Transformed; D002471:Cell Transformation, Neoplastic; D002999:Clone Cells; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology*; D005454:Fluorescence Polarization; D011905:Genes, ras; D008042:Linolenic Acids / Q000494:pharmacology*; D008560:Membrane Fluidity / Q000187:drug effects; D051379:Mice; D015689:Oncogene Protein p21(ras) / Q000235:genetics; D013329:Structure-Activity Relationship; D017965:gamma-Linolenic Acid
chemical_list: D005231:Fatty Acids, Unsaturated; D008042:Linolenic Acids; D017965:gamma-Linolenic Acid; D015689:Oncogene Protein p21(ras)