title: Reduction and inactivation of the sarcoplasmic Ca(2+)-ATPase by 2-mercaptoethanol--contrast to the (Na+,K+) ATPase.
abstract: The sarcoplasmic Ca(2+)-ATPase was reduced with 300 mM 2-mercaptoethanol at elevated temperatures (40-45 degrees C) with a concomitant loss of ATPase activity. The reduction and inactivation of the Ca(2+)-ATPase proceeded rapidly in the absence of Ca2+. The Ca(2+)-ATPase was also inactivated with 2-mercaptoethanol in the presence of diluted SDS (0.4 mg/ml) even at 20 degrees C. In contrast to the (Na+, K+) ATPase, the inactivated Ca(2+)-ATPase in the presence of diluted SDS was sedimented by the centrifugation at 100,000 x g for 30 min.
authors.affiliation: Division of Biology, School of Medicine, University of Occupational and Environmental Health, Kitakyushu, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Mutoh
authors.forename: A
authors.initials: A
authors.lastname: Sugino
authors.forename: K
authors.initials: K
authors.lastname: Higashi
authors.forename: M
authors.initials: M
authors.lastname: Takasugi
authors.forename: A
authors.initials: A
authors.lastname: Kuroiwa
authors.forename: M
authors.initials: M
authors.lastname: Kawamura
mesh_terms: D000818:Animals; D000252:Calcium-Transporting ATPases / Q000378:metabolism*; D008623:Mercaptoethanol / Q000494:pharmacology*; D011817:Rabbits; D012519:Sarcoplasmic Reticulum / Q000201:enzymology*; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*; D013696:Temperature
chemical_list: D008623:Mercaptoethanol; D000252:Calcium-Transporting ATPases; D000254:Sodium-Potassium-Exchanging ATPase