[The evaluation of the effect of formol in the detection of Cryptosporidium sp. oocysts in feces by the methods of Sheater and of the Coprotest].
Frequently it is necessary to search for oocysts of Cryptosporidium sp. in the stools potentially contaminated with human immunodeficiency virus (HIV). For the protection of the laboratory personal handling fecal material formalin has been considered useful. However, this disinfectant has not been evaluated when the stool examinations were performed with the technique of flotation-centrifugation in concentrated solution of sugar and with that of "Coprotest". Our experience indicates that the use of formalin may interfere with the results when the first of these methods is used, but it does not alter the results of the second one. This information is of importance for the future assistential and scientific studies.
Amato Neto
V
V
Laboratório de Investigação Médica-Parasitologia, Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo.
Braz
L M
LM
Campos
R
R
Ogassawara
S
S
Motta
H M
HM
Morandi
N F
NF
Marques
H H
HH
D000163:Acquired Immunodeficiency Syndrome / Q000517:prevention & control / Q000635:transmission; D000818:Animals; D003458:Cryptosporidium / Q000187:drug effects / Q000302:isolation & purification*; D004202:Disinfectants / Q000494:pharmacology*; D005069:Evaluation Studies as Topic; D005243:Feces / Q000382:microbiology / Q000469:parasitology*; D005557:Formaldehyde / Q000494:pharmacology*; D015497:HIV-1; D006801:Humans; D017758:Infectious Disease Transmission, Patient-to-Professional / Q000517:prevention & control; D017056:Medical Laboratory Personnel; D008722:Methods
D004202:Disinfectants; D005557:Formaldehyde
Avaliação da influência do formol na pesquisa de oocistos de Cryptosporidium sp. nas fezes, através do método de Sheater e do "Coprotest".