title: Rapid DNA diagnosis of herpes simplex virus serotypes.
abstract: The presence of nucleotide sequences specific for each of herpes simplex virus (HSV) serotypes was demonstrated. These sequences were applied for dot DNA-DNA hybridization and for PCR for rapid DNA diagnosis of HSV infections. These sequences were found by molecular cloning of HSV-DNA fragments after digestion of DNA by KpnI enzyme. The type 1-specific sequence was found around the 5' end of BamHI B-fragment in the L region of type 1 DNA (corresponds to alpha gene 27, promoter-regulatory region) and the type 2-specific sequence was around the junction region of the L and S of type 2 DNA (corresponds to a' sequence). Both simple dot blot hybridization and PCR of HSV DNA's, employing these type-specific nucleotide sequences, were proven to be much more useful than immunofluorescence in terms of type-specific diagnosis of HSV infections.
authors.affiliation: Department of Pathology, Research Institute for Microbial Diseases, Osaka University, Suita, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Matsumoto
authors.forename: O
authors.initials: O
authors.lastname: Yamada
authors.forename: A
authors.initials: A
authors.lastname: Itagaki
authors.forename: S
authors.initials: S
authors.lastname: Ishida
authors.forename: T
authors.initials: T
authors.lastname: Kamahora
authors.forename: T
authors.initials: T
authors.lastname: Kurimura
mesh_terms: D001483:Base Sequence; D004279:DNA, Viral / Q000235:genetics*; D005455:Fluorescent Antibody Technique; D006561:Herpes Simplex / Q000175:diagnosis / Q000382:microbiology*; D006801:Humans; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D012703:Serotyping; D018139:Simplexvirus / Q000145:classification* / Q000235:genetics
chemical_list: D004279:DNA, Viral