Cooperativity in transactivation between retinoic acid receptor and TFIID requires an activity analogous to E1A.
In embryonal carcinoma (EC) cells retinoic acid (RA) strongly induces transcription from the RA receptor beta 2 (RAR beta 2) promoter through an RA response element (RARE) located in close proximity to the TATA box. Here we demonstrate that recombinant human TATA box-binding protein, hTFIID, and RAR functionally cooperate in transactivation of the RAR beta 2 promoter in EC cells in a strictly RA-dependent manner. We demonstrate that the core domain of hTFIID is sufficient to mediate RAR-dependent transcription and that Drosophila, but not yeast, TFIID can substitute for hTFIID. In COS cells ectopic expression of the E1A protein is a prerequisite for hTFIID and RAR to cooperate in transactivation. We propose a model for transcriptional regulation of the RAR beta 2 promoter in EC cells in which RAR, following activation by RA, functionally interacts with hTFIID via an E1A-like activity present in EC cells.
Berkenstam
A
A
EMBL, Gene Expression Program, Heidelberg, Germany.
Vivanco Ruiz
M M
MM
Barettino
D
D
Horikoshi
M
M
Stunnenberg
H G
HG
D017456:Adenovirus Early Proteins; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002352:Carrier Proteins / Q000502:physiology*; D002460:Cell Line; D002522:Chlorocebus aethiops; D004252:DNA Mutational Analysis; D004331:Drosophila melanogaster / Q000502:physiology; D005786:Gene Expression Regulation; D006801:Humans; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D009856:Oncogene Proteins, Viral / Q000502:physiology; D011401:Promoter Regions, Genetic; D018168:Receptors, Retinoic Acid; D012045:Regulatory Sequences, Nucleic Acid; D012441:Saccharomyces cerevisiae; D013045:Species Specificity; D035362:Transcription Factor TFIID; D014157:Transcription Factors / Q000502:physiology*; D015533:Transcriptional Activation
D017456:Adenovirus Early Proteins; D002352:Carrier Proteins; D009856:Oncogene Proteins, Viral; D018168:Receptors, Retinoic Acid; D035362:Transcription Factor TFIID; D014157:Transcription Factors