Detection of human group C rotaviruses by an enzyme-linked immunosorbent assay using monoclonal antibodies.
An enzyme-linked immunosorbent assay using monoclonal antibodies was established for the detection of human group C rotaviruses. Seventeen clinical samples which were found to contain group C rotaviruses were all strongly positive, whereas 9 samples containing group A rotaviruses and 51 samples lacking rotaviruses were all negative with this test.
Fujii
R
R
Department of Microbiology, Okayama Prefectural Institute for Environmental Science and Public Health, Japan.
Kuzuya
M
M
Hamano
M
M
Yamada
M
M
Yamazaki
S
S
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000956:Antigens, Viral / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D012367:RNA, Viral / Q000032:analysis; D012401:Rotavirus / Q000276:immunology / Q000302:isolation & purification*
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D012367:RNA, Viral