title: Lack of JC viral genomic sequences in multiple sclerosis brain tissue by polymerase chain reaction.
abstract: With DNA extracted from brain specimens from 19 multiple sclerosis, 5 progressive multifocal leukoencephalopathy, 1 Alzheimer's disease, and 8 nonneurological control subjects, polymerase chain reaction was performed using nested sets of primer pairs amplifying segments of the large T and VP1 antigen-encoding sequences of JC virus. Both sequences were detected in each of the 5 brain specimens of progressive multifocal leukoencephalopathy but in none of the 19 multiple sclerosis, 1 Alzheimer's disease, or the 8 control brain specimens.
authors.affiliation: Laboratory of Central Nervous System Studies, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892.
authors.forename: G J
authors.initials: GJ
authors.lastname: Buckle
authors.forename: M S
authors.initials: MS
authors.lastname: Godec
authors.forename: J U
authors.initials: JU
authors.lastname: Rubi
authors.forename: C
authors.initials: C
authors.lastname: Tornatore
authors.forename: E O
authors.initials: EO
authors.lastname: Major
authors.forename: C J
authors.initials: CJ
authors.lastname: Gibbs
authors.forename: D C
authors.initials: DC
authors.lastname: Gajdusek
authors.forename: D M
authors.initials: DM
authors.lastname: Asher
mesh_terms: D001483:Base Sequence; D001921:Brain / Q000382:microbiology; D006801:Humans; D007577:JC Virus / Q000235:genetics / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D009103:Multiple Sclerosis / Q000382:microbiology*; D016133:Polymerase Chain Reaction