Histoenzymic effects of thiophenate and fenbendazole on the absorptive surfaces of Haemonchus contortus.
In vitro alterations induced by a 10 micrograms/ml and 50 micrograms/ml dose each of thiophenate and fenbendazole on the absorptive surfaces of Haemonchus contortus (Nematoda: Trichostrongylidae) were studied. The most significant changes were induced in the gut epithelium. Alkaline phosphatase and adenosine triphosphatase activities were decreased, succinic dehydrogenase activity was increased, while acid phosphatase and glucose-6-phosphatase were completely lost from the intestinal epithelium after treatment with either of the drugs. A stimulatory effect of these two anthelmintics was observe on lactic dehydrogenase and reduced nicotinamide adenine dinucleotide diaphorase distribution. Thiophenate caused an increase in the activities of glutamate dehydrogenase (GDH), glucose-6-phosphate dehydrogenase (G-6-PD) and nonspecific esterases and a decrease in reduced nicotinamide adenine dinucleotide phosphate diaphorase (NADPH-D) activity. Fenbendazole treatment led to the inhibition of GDH, while G-6-PD, NADPH-D, cytochrome oxidase, monoamine oxidase and nonspecific esterase activity remained unaltered in the epithelium.
Kaur
M
M
Department of Zoology, Punjab Agricultural University, Ludhiana, India.
Sood
M L
ML
D000042:Absorption / Q000187:drug effects; D000818:Animals; D000969:Antinematodal Agents / Q000494:pharmacology*; D004950:Esterases / Q000187:drug effects; D005260:Female; D005273:Fenbendazole / Q000494:pharmacology*; D006189:Haemonchus / Q000033:anatomy & histology / Q000187:drug effects* / Q000201:enzymology; D007422:Intestines / Q000187:drug effects; D008297:Male; D010088:Oxidoreductases / Q000187:drug effects; D010636:Phenols / Q000494:pharmacology*; D010744:Phosphoric Monoester Hydrolases / Q000187:drug effects; D013438:Sulfhydryl Compounds
D000969:Antinematodal Agents; D010636:Phenols; D013438:Sulfhydryl Compounds; D005273:Fenbendazole; C042983:thiophenol; D010088:Oxidoreductases; D004950:Esterases; D010744:Phosphoric Monoester Hydrolases