title: A micronucleus-limited sequence family in Tetrahymena thermophila: organization and sequence conservation.
abstract: During macronuclear development in the ciliated protozoan Tetrahymena thermophila, sequence reorganization including sequence loss occurs. Addressing questions about the organization and nucleotide sequence of micronucleus limited regions can lead to insights about mechanisms of DNA rearrangements during macronuclear development as well as mechanisms for the maintenance of the stability of micronucleus-limited sequence families. We have previously identified a moderately repetitive micronucleus-limited sequence family called X-H (family members hybridize to an approximately 450 bp Xbal-HindIII restriction fragment), completely absent from macronuclear DNA. The first member of this family which we isolated is associated with terminal sequences characteristic of a Tel-1 element, a putative micronuclear transposable element. Two additional family members have been isolated which are not closely associated with Tel-1 terminal sequences. We have nucleotide sequence data for three cloned members of the X-H family. This analysis has demonstrated that the longest cloned members of the X-H family share a region of homology of approximately 2,400 bp and are highly conserved, differing only by small insertions or deletions of 100 bp or less. The sequences from one of the sequenced family members flanking the region of homology are themselves mostly micronucleus-limited.
authors.affiliation: Department of Biology, York University, Toronto, Ontario, Canada.
authors.forename: N N
authors.initials: NN
authors.lastname: Tsao
authors.forename: S G
authors.initials: SG
authors.lastname: Tsao
authors.forename: R E
authors.initials: RE
authors.lastname: Pearlman
mesh_terms: D000818:Animals; D001483:Base Sequence; D004251:DNA Transposable Elements; D016054:DNA, Protozoan; D048631:Micronucleus, Germline / Q000378:metabolism*; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D016808:Tetrahymena thermophila / Q000235:genetics*
chemical_list: D004251:DNA Transposable Elements; D016054:DNA, Protozoan