Anti-viral activity of human recombinant heparin-binding proteins HBNF and MK.
Herpes simplex viruses bind to cell surface heparan sulfate proteoglycans, as a first step of viral infection. We report here that two recombinant heparin-binding proteins HBNF and MK inhibit infectivity of human herpes simplex viruses types 1 and 2 and human cytomegalovirus. Carboxymethylated HBNF and MK, which retain affinity for heparin-Sepharose, do not exhibit anti-viral activities. Arguments are presented that anti-viral effects of HBNF and MK are due to the competition for the specific binding to the cell surface heparan sulfate proteoglycans.
Ostrander
M
M
Medical Research Division, Lederle Laboratories, American Cyanamid Company, Pearl River, NY 10965.
Fingar
H
H
Seddon
A
A
Böhlen
P
P
Backer
J
J
D000478:Alkylation; D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D002352:Carrier Proteins / Q000378:metabolism / Q000494:pharmacology*; D016207:Cytokines / Q000378:metabolism / Q000494:pharmacology*; D003587:Cytomegalovirus / Q000187:drug effects*; D006493:Heparin / Q000378:metabolism*; D006801:Humans; D000078792:Midkine; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins / Q000494:pharmacology*; D011506:Proteins / Q000378:metabolism / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000378:metabolism / Q000494:pharmacology*; D018139:Simplexvirus / Q000187:drug effects*; D014709:Vero Cells; D010948:Viral Plaque Assay
D000998:Antiviral Agents; D002352:Carrier Proteins; D016207:Cytokines; C066254:MDK protein, human; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins; D011506:Proteins; D011994:Recombinant Proteins; C066845:pleiotrophin; D000078792:Midkine; D006493:Heparin