[The effect of antimembrane testicular antibodies on Na+, K(+)-ATPase activity in the testicles of rats of different ages].
The effect of large and small doses of rabbit antibodies specific to plasma membranes of the rat testicle cells has been studied in the experiments on Wistar rats of three age groups (preadolescent--aged 20 days, puberal--aged 5-7 months and old--aged 24-26 months). It is stated that incubation of plasma membranes by IgG fraction isolated from antimembrane testicular serum (IgG-ATCSm) in a large dose (43 g of protein G per 125 g of protein of membrane fraction) caused statistically reliable inhibition of Na+, K(+)-ATPase activity in the membranes of testicle cells of puberal and old rats. Preadolescent rats exhibit only a tendency to decrease the activity of this enzyme. Incubation of plasma membranes of testicle cells in rats of different age by small doses of IgG-ATCSm (0.43 g of protein G per 125 g of membrane protein) induced a statistically reliable increase of Na+, K(+)-ATPase activity in puberal and old animals and its slight increase in preadolescent rats. The IgG fraction isolated from normal rabbit serum (IgG-NRS) exerted a less pronounced effect on Na+, K(+)-ATPase activity parallel with retention of a tendency to a decrease of activity under the influence of large doses of the drug and to an increase with introduction of small doses.
Aleksiuk
L I
LI
Sukhina
V S
VS
Zelens'ka
T M
TM
D000375:Aging / Q000276:immunology* / Q000378:metabolism*; D000818:Animals; D000906:Antibodies / Q000494:pharmacology*; D000918:Antibody Specificity / Q000276:immunology; D002462:Cell Membrane / Q000201:enzymology / Q000276:immunology; D004306:Dose-Response Relationship, Immunologic; D007074:Immunoglobulin G / Q000494:pharmacology*; D008297:Male; D011817:Rabbits; D051381:Rats; D017208:Rats, Wistar; D000254:Sodium-Potassium-Exchanging ATPase / Q000187:drug effects* / Q000378:metabolism; D013737:Testis / Q000201:enzymology* / Q000276:immunology*
D000906:Antibodies; D007074:Immunoglobulin G; D000254:Sodium-Potassium-Exchanging ATPase
Vplyv antimembrannykh testykuliarnykh antytil na aktyvnist' Na+, K(+)-ATFazy u testykulakh shchuriv riznoho viku.