title: GLOBIC: a very fast microcomputer program for fingerprinting, characterization and comparison of long nucleotide sequences.
abstract: This paper describes the program GLOBIC, which compares, characterizes and fingerprints even 0.1 Mbase sequences in a few minutes with the aid of an IBM-AT microcomputer. Instead of the nucleotide sequences themselves, GLOBIC compares the local nucleotide or short oligonucleotide compositions. GLOBIC presents two-dimensional maps of contour lines depicting the similarity of two different sequences, a sequence compared to itself, to its complementary sequence or to a random sequence. A vocabulary is presented to translate the typical patterns appearing in the two-dimensional maps into their meanings as relationships between the compared sequences. The application of GLOBIC is demonstrated using several examples from the genomic nucleotide sequences of bacteriophage T7, adenovirus type-2 and Epstein-Barr virus.
authors.affiliation: Institute of Biophysics, Czechoslovak Academy of Sciences, Brno.
authors.forename: J
authors.initials: J
authors.lastname: Mrázek
authors.forename: J
authors.initials: J
authors.lastname: Kypr
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D000465:Algorithms; D001483:Base Sequence; D016172:DNA Fingerprinting / Q000379:methods* / Q000706:statistics & numerical data; D004279:DNA, Viral / Q000235:genetics; D004854:Herpesvirus 4, Human / Q000235:genetics; D016415:Sequence Alignment / Q000379:methods / Q000706:statistics & numerical data; D012984:Software; D013604:T-Phages / Q000235:genetics
chemical_list: D004279:DNA, Viral