Mapping of GABAA receptor alpha 5 and alpha 6 subunit-like immunoreactivity in rat brain.
The distribution of the alpha 5 and alpha 6 subunits of the GABAA receptor has been mapped in rat brain using affinity-purified antibodies generated against peptide sequences unique to the respective polypeptides. alpha 5 Subunit-like immunoreactivity was of low density but was distributed across several cell groups including cortical interneurones, hippocampal CA3 pyramidal neurones, the anterior thalamic reticular nucleus and cerebellar Purkinje neurones. alpha 6 Subunit-like immunoreactivity was observed in high density in cerebellar granule cells. These patterns are compatible with in situ hybridisation studies and provide a further anatomical substrate for GABAA receptor heterogeneity in the CNS.
Thompson
C L
CL
Department of Pharmaceutical Chemistry, School of Pharmacy, London, UK.
Bodewitz
G
G
Stephenson
F A
FA
Turner
J D
JD
D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000378:metabolism*; D001931:Brain Mapping; D007150:Immunohistochemistry; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D011963:Receptors, GABA-A / Q000378:metabolism*
D012333:RNA, Messenger; D011963:Receptors, GABA-A