Antibodies directed against synthetic peptides distinguish between the catalytic subunits of protein phosphatases 1 and 2A.
Two antipeptide antibodies (designated type 1 antibody and type 2A antibody) were raised against synthetic peptides, Cys-Thr-Pro-Pro-Arg-Asn-Ser-Ala-Lys-Ala-Lys-Lys and Cys-Val-Thr-Arg-Arg-Thr-Pro-Asp-Try-Phe-Leu, corresponding to the carboxyl termini of the catalytic subunits of protein phosphatase 1 and phosphatase 2A (Cys was added for specific coupling to carrier protein). These antipeptide antibodies were highly specific and were useful in discriminating between protein phosphatase 1 and phosphatase 2A in crude extracts or purified preparations. Type 2A antibody reacted with both native and denatured protein phosphatase 2A whereas under the same condition type 1 antibody reacted only with denatured protein phosphatase 1.
Wang
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W
Department of Pharmacology, Medical College of Ohio, Toledo 43699.
Lane
R D
RD
Schlender
K K
KK
D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D008969:Molecular Sequence Data; D010455:Peptides / Q000276:immunology; D010749:Phosphoprotein Phosphatases / Q000276:immunology*; D054645:Protein Phosphatase 1; D054648:Protein Phosphatase 2; D011817:Rabbits
D010455:Peptides; D010749:Phosphoprotein Phosphatases; D054645:Protein Phosphatase 1; D054648:Protein Phosphatase 2