Protection by salvianolic acid A against adriamycin toxicity on rat heart mitochondria.
It was found that salvianolic acid A (Sai A) has potent antioxidant activity. The effects of Sai A on adriamycin-induced heart mitochondrial toxicity of rats in vitro and on adriamycin antitumor activity are investigated in this article. Malondialdehyde (MDA) formation and membrane rigidification of rat heart mitochondria intoxicated with adriamycin were significantly reduced by Sai A. In the electron spin resonance (ESR) studies, Sai A has no significant effect on the formation of adriamycin semiquinone radicals (AQ.), while hydroxyl radicals generated by electron transfer from AQ. to H2O2 were scavenged by Sai A dose-dependently. On the other hand, Sai A was shown to have no effects on the antitumor activity of adriamycin in cultured L1210 ascitic tumor cells and in mice with P388 ascite tumor. These results indicate that Sai A protects against adriamycin induced heart mitochondrial toxicity of rats, while Sai A has no antagonizing effect on the antitumor activity of adriamycin.
Lin
T J
TJ
Institute of Materia Medica, Chinese Academy of Medical Sciences, Beijing.
Liu
G T
GT
Liu
Y
Y
Xu
G Z
GZ
D000818:Animals; D000970:Antineoplastic Agents / Q000627:therapeutic use; D002109:Caffeic Acids / Q000494:pharmacology*; D002462:Cell Membrane / Q000187:drug effects; D004317:Doxorubicin / Q000494:pharmacology / Q000627:therapeutic use / Q000633:toxicity*; D004578:Electron Spin Resonance Spectroscopy; D004579:Electron Transport; D005609:Free Radicals; D006861:Hydrogen Peroxide / Q000378:metabolism; D006878:Hydroxides / Q000378:metabolism; D017665:Hydroxyl Radical; D007773:Lactates / Q000494:pharmacology*; D007939:Leukemia L1210 / Q000188:drug therapy; D008297:Male; D051379:Mice; D008929:Mitochondria, Heart / Q000187:drug effects* / Q000648:ultrastructure; D009374:Neoplasms, Experimental / Q000188:drug therapy; D051381:Rats; D011919:Rats, Inbred Strains; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents; D002109:Caffeic Acids; D005609:Free Radicals; D006878:Hydroxides; D007773:Lactates; D017665:Hydroxyl Radical; C066201:salvianolic acid A; D004317:Doxorubicin; D006861:Hydrogen Peroxide