title: Rapid determination of human cytomegalovirus susceptibility to ganciclovir directly from clinical specimen primocultures.
abstract: We were able to rapidly determine the susceptibility of human cytomegalovirus to ganciclovir using a late antigen synthesis reduction assay directly on primocultures of clinical specimens. This test was compared with a conventional susceptibility assay which was performed with cell-free virus obtained after cytomegalovirus isolation and in vitro passages. Both tests produced similar results. The rapid test, unlike conventional assays, is able to provide a result within 5 days after receipt of the specimen and could thus play a direct role in the therapeutic decision.
authors.affiliation: Department of Virology, Claude Bernard Hospital, Paris, France.
authors.forename: J M
authors.initials: JM
authors.lastname: Pepin
authors.forename: F
authors.initials: F
authors.lastname: Simon
authors.forename: A
authors.initials: A
authors.lastname: Dussault
authors.forename: G
authors.initials: G
authors.lastname: Collin
authors.forename: M C
authors.initials: MC
authors.lastname: Dazza
authors.forename: F
authors.initials: F
authors.lastname: Brun-Vezinet
mesh_terms: D003587:Cytomegalovirus / Q000187:drug effects* / Q000302:isolation & purification; D003586:Cytomegalovirus Infections / Q000188:drug therapy / Q000382:microbiology; D004352:Drug Resistance, Microbial; D005069:Evaluation Studies as Topic; D015774:Ganciclovir / Q000494:pharmacology*; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods*; D013997:Time Factors; D014776:Virus Cultivation
chemical_list: D015774:Ganciclovir