Improved method for coliphage detection based on beta-galactosidase induction.
An improved method for coliphage detection based on the induction of beta-galactosidase in Escherichia coli is described. Upon infection by coliphages, the cells are lysed and a stable indolyl product that is dark blue becomes visible within each plaque. The improved method is compared to the proposed coliphage detection procedure described in Standard Methods for the Examination of Water and Wastewater.
Ijzerman
M M
MM
Department of Crop and Soil Environmental Sciences, Virginia Polytechnic Institute and State University, Blacksburg 24061-0404.
Hagedorn
C
C
D003090:Coliphages / Q000254:growth & development / Q000302:isolation & purification*; D004790:Enzyme Induction; D004926:Escherichia coli / Q000201:enzymology; D005697:Galactosides; D007211:Indoles; D007544:Isopropyl Thiogalactoside; D010948:Viral Plaque Assay; D014871:Water Microbiology; D001616:beta-Galactosidase / Q000096:biosynthesis
D005697:Galactosides; D007211:Indoles; D007544:Isopropyl Thiogalactoside; D001616:beta-Galactosidase; C044888:5-bromo-4-chloro-3-indolyl beta-galactoside