title: [Quantitative skeletal muscle pathology of aging regarding ragged-red fibers and cytochrome c oxidase-negative fibers].
abstract: A statistical analysis of mitochondrial abnormality of aging in human skeletal muscle fibers was performed. Sixty one muscle samples were obtained from patients with acute medical illness autopsied strictly within 2 hours after death, or with orthopedic or surgical diseases biopsied with informed consents. The patients aged from 16 to 89, averaging 58 +/- 21 years in males and 21 to 92, averaging 55 +/- 20 years in females. Sections were stained by modified Gomori's trichrome, succinate dehydrogenase and cytochrome c oxidase-negative [CCO(-)] fibers approximately in 10,000 fibers in each muscle were evaluated. Both RRF and CCO (-) fibers were not observed below the fourth decade, but sequentially increased with age, especially after the seventh decade. The incidence of CCO (-) fibers was higher than that of RRF. RRF did not necessarily correspond to CCO (-) fibers. The present quantitative pathological result is a useful tool to evaluate the mitochondrial function in fresh human skeletal muscles by the age.
authors.affiliation: Fourth Department of Medicine, Aichi Medical University.
authors.forename: H
authors.initials: H
authors.lastname: Suoh
authors.forename: K
authors.initials: K
authors.lastname: Sahashi
authors.forename: T
authors.initials: T
authors.lastname: Ibi
authors.forename: M
authors.initials: M
authors.lastname: Tashiro
authors.forename: T
authors.initials: T
authors.lastname: Mitsuma
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000473:pathology*; D001706:Biopsy; D003576:Electron Transport Complex IV / Q000378:metabolism*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D008931:Mitochondria, Muscle / Q000473:pathology; D009132:Muscles / Q000201:enzymology / Q000473:pathology*
chemical_list: D003576:Electron Transport Complex IV