title: Lack of in vitro interference between SA-11 rotavirus and attenuated poliovirus type 1.
abstract: To determine if rotavirus interferes with the multiplication of poliovirus, and hence the efficiency of the polio vaccine, the effect of timing and concentration of Simian rotavirus (SSA-11) on polio-1 infection in MA-104 cells was studied in vitro by evaluating the cytopathic effect, the reduction of the infectivity titers and the visualization of viral particles by electron microscopy. We found that poliovirus 1 was able to replicate when the challenge dose was administered within the first 8 h following SA-11 infection and with titers ten times lower than those of rotavirus. Hence, non-interference effect was observed in this in vitro model.
authors.affiliation: Clinical Infectious Diseases Research Unit, Instituto Mexicano del Seguro Social, D.F.
authors.forename: J P
authors.initials: JP
authors.lastname: Guiscafré Gallardo
authors.forename: C
authors.initials: C
authors.lastname: Zamora Zavala
authors.forename: T
authors.initials: T
authors.lastname: Alvarez y Muñoz
authors.forename: O
authors.initials: O
authors.lastname: Muñoz
mesh_terms: D000818:Animals; D002460:Cell Line; D003588:Cytopathogenic Effect, Viral; D007106:Immune Sera; D007668:Kidney; D008253:Macaca mulatta; D017955:Poliovirus / Q000276:immunology / Q000502:physiology* / Q000648:ultrastructure; D011055:Poliovirus Vaccine, Oral; D011817:Rabbits; D012401:Rotavirus / Q000276:immunology / Q000502:physiology* / Q000648:ultrastructure; D018139:Simplexvirus / Q000502:physiology; D014709:Vero Cells; D014762:Viral Interference; D014771:Virion / Q000648:ultrastructure; D014779:Virus Replication
chemical_list: D007106:Immune Sera; D011055:Poliovirus Vaccine, Oral