Use of diethyldithiocarbamate for quantitative determination of tellurite uptake by bacteria.
We have developed a simple method for the quantitative determination of tellurite in biological media. This assay is suitable for studying tellurite uptake in bacteria and overcomes the problems of older techniques which are time consuming and labor intensive. In earlier protocols diethyldithiocarbamate was reacted with tellurite and the resulting complex was extracted into organic solvents before spectrophotometric determination. In this study, diethyldithiocarbamate was incubated with tellurite at neutral pH to form a yellow colloidal solution. The absorbance of the aqueous yellow sol was used to determine tellurite concentrations in the range of 1 to 50 micrograms/ml (4 to 200 microM) without the need for solvent extraction.
Turner
R J
RJ
Department of Biochemistry, University of Alberta, Edmonton, Canada.
Weiner
J H
JH
Taylor
D E
DE
D001151:Arsenic / Q000378:metabolism; D018053:Arsenites; D001692:Biological Transport; D004050:Ditiocarb; D004926:Escherichia coli / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D010957:Plasmids; D012643:Selenium / Q000378:metabolism; D013057:Spectrum Analysis; D013691:Tellurium / Q000032:analysis* / Q000378:metabolism
D018053:Arsenites; D004050:Ditiocarb; D012643:Selenium; C026660:tellurous acid; C015001:arsenite; D001151:Arsenic; D013691:Tellurium