Origin of brain 2',3'-cyclic-nucleotide 3'-phosphodiesterase doublet.
The present study established that 2',3'-cyclic-nucleotide 3'-phosphodiesterase doublet common to mammalian brain originates from an alternative splicing. Peptides specific to the predicted larger translation product were synthesized and antisera against these peptides were prepared. Immunostaining of SDS/PAGE blots showed that the antisera react with the larger protein, but not with the smaller protein, of 2',3'-cyclic-nucleotide 3'-phosphodiesterase doublet in all mammals studied.
Kurihara
T
T
Institute of Life Science, Soka University, Tokyo, Japan.
Tohyama
Y
Y
Yamamoto
J
J
Kanamatsu
T
T
Watanabe
R
R
Kitajima
S
S
D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000166:cytology / Q000201:enzymology*; D002417:Cattle; D006801:Humans; D015151:Immunoblotting; D051379:Mice; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis; D014176:Protein Biosynthesis; D012326:RNA Splicing; D051381:Rats; D017385:Sequence Homology; D013045:Species Specificity
D010455:Peptides; D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases