Protein interactions with urea and guanidinium chloride. A calorimetric study.
The interaction of urea and guanidinium chloride with proteins has been studied calorimetrically by titrating protein solutions with denaturants at various fixed temperatures, and by scanning them with temperature at various fixed concentrations of denaturants. It has been shown that the observed heat effects can be described in terms of a simple binding model with independent and similar binding sites. Using the calorimetric data, the number of apparent binding sites for urea and guanidinium chloride have been estimated for three proteins in their unfolded and native states (ribonuclease A, hen egg white lysozyme and cytochrome c). The intrinsic and total thermodynamic characteristics of their binding (the binding constant, the Gibbs energy, enthalpy, entropy and heat capacity effect of binding) have also been determined. It is found that the binding of urea and guanidinium chloride by protein is accompanied by a significant decrease of enthalpy and entropy. At all concentrations of denaturants the enthalpy term slightly dominates the entropy term in the Gibbs energy function. Correlation analysis of the number of binding sites and structural characteristics of these proteins suggests that the binding sites for urea and guanidinium chloride are likely to be formed by several hydrogen bonding groups. This type of binding of the denaturant molecules should lead to a significant restriction of conformational freedom within the polypeptide chain. This raises a doubt as to whether a polypeptide chain in concentrated solutions of denaturants can be considered as a standard of a random coil conformation.
Makhatadze
G I
GI
Department of Biology, Johns Hopkins University, Baltimore, MD 21218.
Privalov
P L
PL
D002151:Calorimetry; D003574:Cytochrome c Group / Q000737:chemistry; D006146:Guanidines / Q000737:chemistry*; D006860:Hydrogen Bonding; D009113:Muramidase / Q000737:chemistry; D011489:Protein Denaturation; D011506:Proteins / Q000737:chemistry*; D012260:Ribonucleases / Q000737:chemistry; D013696:Temperature; D013816:Thermodynamics; D014508:Urea / Q000737:chemistry*
D003574:Cytochrome c Group; D006146:Guanidines; D011506:Proteins; D014508:Urea; D012260:Ribonucleases; D009113:Muramidase