Detection of herpes simplex virus in gingival tissue.
The presence of herpes simplex virus (HSV) antigens was shown by immunofluorescence staining in 26 of 66 (39.3%) specimens of clinically healthy gingiva, but only one sample contained infectious virus. HSV DNA sequences were clearly identified in intact gingival cells by dot blot hybridization in one specimen, and a weak pattern in a second one. Both specimens harbored viral antigens. These findings of viral genome and protein expression suggest that the virus is present in the latent form in the gingiva.
Amit
R
R
Virology Department, Hebrew University Hadassah Medical School, Jerusalem, Israel.
Morag
A
A
Ravid
Z
Z
Hochman
N
N
Ehrlich
J
J
Zakay-Rones
Z
Z
D000328:Adult; D001345:Autoradiography; D004247:DNA / Q000032:analysis; D015342:DNA Probes; D004279:DNA, Viral / Q000032:analysis; D005455:Fluorescent Antibody Technique; D016679:Genome, Viral; D005881:Gingiva / Q000382:microbiology*; D006801:Humans; D015151:Immunoblotting; D009693:Nucleic Acid Hybridization; D012016:Reference Values; D018139:Simplexvirus / Q000235:genetics / Q000302:isolation & purification*; D014776:Virus Cultivation / Q000379:methods
D015342:DNA Probes; D004279:DNA, Viral; D004247:DNA