Electrophoretic transfer of DNA from gels to filters by using a homemade apparatus.
Electroblotting is a rapid and high-efficiency method of transferring DNA from gels to the variety of membranes available, in very short time frames when compared with capillary-blotting techniques and with much less preparation required. A simple apparatus made of two food savers, one dialysis membrane, one sponge, and two removable electrodes was used to transfer DNA molecules up to 21 kb, from agarose gels to nitrocellulose filters, in less than 30 min. A recombinant pAT153 plasmid carrying the human cytomegalovirus BamH I-P fragment (7.2 kb) was rapidly identified by hybridization, using our simplified transfer procedure.
Pramatarova
A
A
Research and Virology Center, Armand-Frappier Institute, Laval-des-Rapides, Québec, Canada.
Michaud
L
L
Gagnon
F
F
Hamelin
C
C
D015139:Blotting, Southern / Q000295:instrumentation* / Q000379:methods; D003101:Collodion; D003587:Cytomegalovirus / Q000235:genetics; D004247:DNA / Q000032:analysis*; D004274:DNA, Recombinant / Q000032:analysis; D004279:DNA, Viral / Q000235:genetics; D004586:Electrophoresis / Q000295:instrumentation* / Q000379:methods; D004587:Electrophoresis, Agar Gel; D005782:Gels; D008567:Membranes, Artificial
D004274:DNA, Recombinant; D004279:DNA, Viral; D005782:Gels; D008567:Membranes, Artificial; D003101:Collodion; D004247:DNA