Central effects of tricyclic compounds on the endocrine system--an in vitro study.
The present study involves the effects on corticotropin (ACTH) release of neuro- and thymoleptic tricyclic antidepressant compounds (TrcACs: chlorpromazine, promethazine, haloperidol, imipramine, amitriptyline) and their interactions with lysine-8-vasopressin (LVP) and corticosterone (B). As an in vitro model, 14-day monolayer pituitary cell cultures of Wistar rats were employed. The ACTH concentrations of the supernatant media were measured by radioimmunoassay. TrcACs augmented ACTH release; their combination with LVP, however, did not result in further stimulation; moreover, when combined with TrcACs + LVP, B did not inhibit, but rather paradoxically increased their ACTH-releasing action. As none of these phenomena were followed by relevant changes in intracellular cyclic adenosine monophosphate content, the mechanism of action may be proposed to involve a protein kinase C route.
Julesz
J
J
Endocrine Unit, A. Szent-Györgyi Medical University, Szeged, Hungary.
Gálfi
M
M
Molnár
J
J
Vecsernyés
M
M
D000324:Adrenocorticotropic Hormone / Q000378:metabolism*; D000639:Amitriptyline / Q000494:pharmacology; D000818:Animals; D000929:Antidepressive Agents, Tricyclic / Q000494:pharmacology*; D002478:Cells, Cultured; D002746:Chlorpromazine / Q000494:pharmacology; D003345:Corticosterone / Q000494:pharmacology; D000242:Cyclic AMP / Q000378:metabolism*; D004347:Drug Interactions; D006220:Haloperidol / Q000494:pharmacology; D007099:Imipramine / Q000494:pharmacology; D008236:Lypressin / Q000494:pharmacology; D010902:Pituitary Gland / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D011398:Promethazine / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar
D000929:Antidepressive Agents, Tricyclic; D000639:Amitriptyline; D008236:Lypressin; D000324:Adrenocorticotropic Hormone; D000242:Cyclic AMP; D011398:Promethazine; D006220:Haloperidol; D007099:Imipramine; D002746:Chlorpromazine; D003345:Corticosterone