title: Effects of actin filaments on fibrin clot structure and lysis.
abstract: The muscle and cytoskeletal protein actin is released from cells as a consequence of cell death and interacts with components of the hemostatic and fibrinolytic systems, including platelets, plasmin, and fibrin. We report here that incorporation of actin filaments into fibrin clots changes their viscoelastic properties by increasing their shear modulus at low deforming stresses and by nearly eliminating their tendency to become more rigid with increasing deformation (ie, exhibit strain-hardening). The viscoelastic effects depended on the length of the actin filaments as shown by the effects of the plasma filament-severing protein, gelsolin. Binding of actin to fibrin clots also varied with actin filament length. The plasma actin-binding proteins gelsolin and vitamin D-binding protein reduced, but did not eliminate, the incorporation of actin in the clot. Fluorescence microscopy showed a direct association of rhodamine-labeled actin filaments with the fibrin network. Incubation of clots containing long actin filaments in solutions containing physiologic concentrations of gelsolin (2 mumol/L) released 60% of the actin trapped in the clot. Reduction of the actin content of a fibrin clot by incubation in a gelsolin-containing solution resulted in an increased rate of clot lysis. The ability of plasma gelsolin to shorten actin filaments may therefore be of physiologic and potentially of therapeutic importance insofar as gelsolin-mediated diffusion of actin from the clot may restore the clot's rheologic properties and render it more sensitive to the lytic action of plasmin.
authors.affiliation: Division of Experimental Medicine, Brigham and Women's Hospital, Boston, MA 02115.
authors.forename: P A
authors.initials: PA
authors.lastname: Janmey
authors.forename: J A
authors.initials: JA
authors.lastname: Lamb
authors.forename: R M
authors.initials: RM
authors.lastname: Ezzell
authors.forename: S
authors.initials: S
authors.lastname: Hvidt
authors.forename: S E
authors.initials: SE
authors.lastname: Lind
mesh_terms: D000199:Actins / Q000737:chemistry / Q000378:metabolism*; D001665:Binding Sites; D002135:Calcium-Binding Proteins / Q000494:pharmacology; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D003850:Deoxyribonuclease I / Q000378:metabolism; D004548:Elasticity; D005337:Fibrin / Q000737:chemistry* / Q000378:metabolism*; D005342:Fibrinolysis; D005456:Fluorescent Dyes; D018260:Gelsolin; D006801:Humans; D046911:Macromolecular Substances; D008840:Microfilament Proteins / Q000494:pharmacology; D008856:Microscopy, Fluorescence; D012235:Rhodamines; D014783:Viscosity; D014809:Vitamin D-Binding Protein / Q000494:pharmacology
chemical_list: D000199:Actins; D002135:Calcium-Binding Proteins; D005456:Fluorescent Dyes; D018260:Gelsolin; D046911:Macromolecular Substances; D008840:Microfilament Proteins; D012235:Rhodamines; D014809:Vitamin D-Binding Protein; D005337:Fibrin; D003850:Deoxyribonuclease I