[Interference of platelet-activating factor antagonist, BN50739 with the releasing of inflammatory mediators from anaphylactic lung of guinea pig].
BN50739 is a new and potent PAF antagonist. In this experiment, we have further confirmed that it is a selective PAF antagonist by means of rabbit platelet aggregating test. And also, when BN50739 was applied, in the concentration of 10 uM, to the isolated lung of guinea pig sensitized by ovalbumin, we observed that the amount of histamine and thromboxane A2 released was a little less (about 19.0% and 19.2%, respectively) than that of controls (P < 0.05). Whereas, the ratio of PGI2/TxA2 was increased by 17.6% comparing to that in control group (P < 0.05). But the level of LTC4 remained unchanged regardless of the presence of BN50739. We conclude that PAF may play a role in allergic reaction.
Zhang
J N
JN
Respiratory disease research lab Union hospital of Tongji medical university, Wuhan.
D000542:Alveolitis, Extrinsic Allergic / Q000139:chemically induced / Q000378:metabolism*; D000818:Animals; D001381:Azepines / Q000494:pharmacology*; D005260:Female; D006168:Guinea Pigs; D006632:Histamine / Q000378:metabolism; D008168:Lung / Q000378:metabolism; D008297:Male; D010047:Ovalbumin; D010972:Platelet Activating Factor / Q000037:antagonists & inhibitors*; D011460:Prostaglandins F / Q000378:metabolism; D013189:SRS-A / Q000378:metabolism; D013929:Thromboxane B2 / Q000378:metabolism; D014230:Triazoles / Q000494:pharmacology*
D001381:Azepines; D010972:Platelet Activating Factor; D011460:Prostaglandins F; D013189:SRS-A; D014230:Triazoles; C064509:BN 50739; D013929:Thromboxane B2; D006632:Histamine; D010047:Ovalbumin; C100006:prostaglandin F1