Crystallization and preliminary crystallographic study of an octa-heme cytochrome c3 from Desulfovibrio desulfuricans Norway.
An octa-heme cytochrome c3, isolated as a dimeric molecule of about 30 kDa from the anaerobic bacteria Desulfovibro desulfuricans Norway, has been crystallized in a form suitable for atomic resolution X-ray structural investigations. The crystals are trigonal, space group P3(1)21 (or its enantiomorph P3(2)21), with cell dimensions: a = b = 72.9 A c = 62.7 A. The asymmetric unit contains most probably one monomer and a solvent content of about 60%. Under this assumption, the crystallographic 2-fold axis relates the two subunits of the dimer. Diffraction extends to 2.0 A.
Czjzek
M
M
Laboratoire de Cristallographie et Cristallisation des Macromolécules Biologiques-CNRS, URA 1296, Université d'Aix-Marseille II, Faculté de Médecine Nord, France.
Guerlesquin
F
F
Roig
V
V
Payan
F
F
Bruschi
M
M
Haser
R
R
D003460:Crystallization; D003574:Cytochrome c Group / Q000737:chemistry*; D003901:Desulfovibrio / Q000737:chemistry*; D011487:Protein Conformation; D014961:X-Ray Diffraction
D003574:Cytochrome c Group; C017165:cytochrome c(3)