Increase in nucleoside diphosphatase in rat brain striatum lesioned with kainic acid.
The activity of ammoniagenesis from guanine nucleotides was found to increase significantly in rat brain after infusion of kainic acid into the striatum. Among the enzymes involved in degrading guanine nucleotides, nucleoside diphosphatase was markedly increased in the lesioned striatum. The enzyme activity began to increase 2 days after the infusion, and reached the maximum on the 13th day, the level being 4 times as high as that of the intact contralateral region. The increased activity was due to Type L enzyme, judging from its substrate specificity. Puromycin and cycloheximide inhibited this increase, indicating that the increased activity resulted from an increase in the net synthesis of the enzyme. These findings suggest that Type L NDPase might play some important roles in gliosis after neuronal lesion.
Miyamoto
S
S
Institute for Protein Research, Osaka University, Japan.
Matsuda
Y
Y
Sano
S
S
Shiraki
H
H
Nakagawa
H
H
D017766:Acid Anhydride Hydrolases; D000641:Ammonia / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000201:enzymology*; D003342:Corpus Striatum / Q000378:metabolism*; D003513:Cycloheximide / Q000494:pharmacology; D005911:Gliosis; D007608:Kainic Acid / Q000008:administration & dosage / Q000633:toxicity*; D008297:Male; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism*; D011691:Puromycin / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar
D011691:Puromycin; D000641:Ammonia; D003513:Cycloheximide; D010744:Phosphoric Monoester Hydrolases; D017766:Acid Anhydride Hydrolases; C023837:nucleoside-diphosphatase; D007608:Kainic Acid