Orthotopic reconstitution of human small-cell lung carcinoma after intravenous transplantation in SCID mice.
We have constructed an orthotopically reconstituted model of human small-cell lung carcinoma (SCLC) by intravenous transplantation in severe combined immunodeficient (SCID) mice. Two human SCLC xenografts, H-69 and Lu-130, were disaggregated and injected through the tail vein of SCID mice. Human SCLCs were orthotopically reconstituted with multi-focal lung tumor growth in all SCID mice after intravenous injection of 5 x 10(6) tumor cells per mouse. The heart and liver were also seeded with actively growing SCLC. This orthotopic reconstitution model of human SCLC in SCID mice should be useful for further studies on the biological behavior and treatment of human SCLC.
Kuo
T H
TH
Department of Surgery, School of Medicine, Keio University, Tokyo, Japan.
Kubota
T
T
Watanabe
M
M
Furukawa
T
T
Kase
S
S
Tanino
H
H
Nishibori
H
H
Saikawa
Y
Y
Teramoto
T
T
Ihsibiki
K
K
D000818:Animals; D018288:Carcinoma, Small Cell / Q000473:pathology*; D002455:Cell Division; D006338:Heart Neoplasms / Q000473:pathology / Q000556:secondary*; D006801:Humans; D008113:Liver Neoplasms / Q000473:pathology / Q000556:secondary*; D008175:Lung Neoplasms / Q000473:pathology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D016513:Mice, SCID; D009368:Neoplasm Transplantation; D014183:Transplantation, Heterologous