Regulation of beta-endorphin receptor expression in mouse spleen cells with Con A and rIL-2.
The expression of the beta-endorphin receptor on both activated and unstimulated mouse spleen cells was studied. Results showed that unstimulated cells have only one type of beta-endorphin receptor with a specific low affinity (Kd = 1.034 +/- 0.0237 x 10(-7) M, 25,000 sites/cell). After Con A stimulation, cells express two types of receptors, one with a low affinity (Kd = 1.034 +/- 0.024 x 10(-7) M, 320,000 sites/cell) and the other with a high affinity (Kd = 1.052 +/- 0.033 x 10(-9) M, 49,000 sites/cell). The kinetic experiments during 4 days after Con A activation indicated that the receptor of high affinity emerged from 24 to 72 h, while the low affinity one increased in number after stimulation. The receptor numbers of both high and low affinity ones reached a maximum peak at 72 h, then began to decline. The addition of exogenous rIL-2 depressed the Con A-induced increment of the receptor numbers of both the high and low affinity ones, but enhanced the proliferative response of the cells. It is suggested that the degree of the expression of the receptors does not simply depend on the mitogenic degree of the cells. In addition, our experiment demonstrated that splenocytes cultured in medium with or without Con A or Con A + rIL-2 for 96 h did not secrete any detectable amount of beta-endorphin with use of the RIA assay, which is sensitive enough to detect the much lower levels of beta-endorphin than that necessary for biological effects. We suggest that the expression of the high affinity beta-endorphin receptor on the activated T-lymphocytes may have to precede the production of IL-2 to potentiate the T-cell proliferative response. The mechanisms and modes of interaction between the neuroendocrine system and the immune system were discussed.
Jia
L
L
Department of Medicine III, Osaka University Medical School, Japan.
Hara
H
H
Okochi
T
T
Negoro
S
S
D000818:Animals; D001667:Binding, Competitive; D002478:Cells, Cultured; D003208:Concanavalin A; D015536:Down-Regulation; D007376:Interleukin-2 / Q000096:biosynthesis / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D011869:Radioligand Assay; D011957:Receptors, Opioid / Q000032:analysis*; D011994:Recombinant Proteins / Q000494:pharmacology; D013154:Spleen / Q000737:chemistry*; D001615:beta-Endorphin / Q000378:metabolism*
D007376:Interleukin-2; D011957:Receptors, Opioid; D011994:Recombinant Proteins; D003208:Concanavalin A; D001615:beta-Endorphin