title: Renal cortical endosomes participate in the degradation of insulin.
abstract: It has long been thought that metabolism of insulin by kidney proximal tubule cells takes place only in lysosomes. But previous studies with the perfused rat kidney and a proximal tubule-like cultured kidney cell line suggested that insulin degradation occurred in a nonlysosomal compartment. In this study we show that endosomes isolated from rat kidney cortex degrade insulin and that this process is ATP and pH dependent.
authors.affiliation: Stanford University, Calif.
authors.forename: J
authors.initials: J
authors.lastname: Fawcett
authors.forename: B
authors.initials: B
authors.lastname: Frank
authors.forename: R
authors.initials: R
authors.lastname: Rabkin
mesh_terms: D000255:Adenosine Triphosphate / Q000502:physiology; D000818:Animals; D003574:Cytochrome c Group / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007328:Insulin / Q000378:metabolism*; D007668:Kidney / Q000201:enzymology; D007672:Kidney Cortex / Q000378:metabolism* / Q000648:ultrastructure; D008247:Lysosomes / Q000378:metabolism; D015388:Organelles / Q000502:physiology*; D051381:Rats
chemical_list: D003574:Cytochrome c Group; D007328:Insulin; D000255:Adenosine Triphosphate