Involvement of a d-type oxidase in the Na(+)-motive respiratory chain of Escherichia coli growing under low delta mu H+ conditions.
An attempt has been made to find out which of the two terminal oxidases, the d-type or the o-type, operates as a Na+ pump in Escherichia coli grown at low delta mu H+ conditions. For this purpose, mutants lacking either d or o oxidase have been studied. It is shown that a d-,o+ mutant grows slowly or does not grow at all under low delta mu H+ conditions (alkaline or protonophore-containing growth media were used). Inside-out subcellular vesicles from the d-,o+ mutant cannot oxidize ascorbate and TMPD, and cannot transport Na+ when succinate is oxidized in the presence of a protonophore. The same vesicles are found to transport Na+ when NADH is oxidized as if the Na(+)-motive NADH-quinone oxidase were operative. On the other hand, a mutant lacking o oxidase (d+,o-) grows at low delta mu H+ conditions as fast as the maternal E. coli strain containing both d and o oxidases. Corresponding vesicles oxidize ascorbate and TMPD as well as succinate, the oxidations being coupled to the protonophore-stimulated Na+ transport. Growth in the presence of a protonophore is found to induce a strong increase in the d oxidase level in the maternal d+,o+ E.coli strain. It is concluded that oxidase of the d-type, rather than of the o-type, operates as a Na+ pump in E. coli grown under conditions unfavorable for the H+ cycle.
Avetisyan
A V
AV
Department of Bioenergetics, A.N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Russia.
Bogachev
A V
AV
Murtasina
R A
RA
Skulachev
V P
VP
D004926:Escherichia coli / Q000201:enzymology* / Q000254:growth & development; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D010088:Oxidoreductases / Q000378:metabolism*; D045322:Photosynthetic Reaction Center Complex Proteins / Q000378:metabolism*; D012964:Sodium / Q000378:metabolism*; D000254:Sodium-Potassium-Exchanging ATPase; D013057:Spectrum Analysis; D013379:Substrate Specificity
D045322:Photosynthetic Reaction Center Complex Proteins; D012964:Sodium; D010088:Oxidoreductases; C020094:duroquinol oxidase; D000254:Sodium-Potassium-Exchanging ATPase