Effect of Cu(2+)-ascorbic acid on lipid peroxidation, Mg(2+)-ATPase activity and spectrin of RBC membrane and reversal by erythropoietin.
The effect of erythropoietin (Ep), a glycoprotein hormone, has been studied on lipid peroxidation induced by Cu2+ and ascorbate in vitro, Mg2+ ATPase activity and spectrin of RBC membrane. Our present investigation reveals that Cu2+ and ascorbic acid increases lipid peroxidation of RBC membrane significantly. It has further been observed that under the same experimental condition spectrin, a major cytoskeleton membrane protein, and Mg(2+)-ATPase activity of RBC membrane decrease significantly. However, exogenous administration of Ep completely restores lipid peroxidation and Mg(2+)-ATPase activity and partially recovers spectrin of RBC membrane.
Chattopadhyay
A
A
Guha Institute of Biochemistry, Calcutta, India.
Das Choudhury
T D
TD
Basu
M K
MK
Datta
A G
AG
D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology*; D017301:Ca(2+) Mg(2+)-ATPase / Q000097:blood*; D003300:Copper / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000378:metabolism*; D004921:Erythropoietin / Q000494:pharmacology*; D000431:Ethanol / Q000494:pharmacology; D016166:Free Radical Scavengers; D005609:Free Radicals / Q000097:blood; D006041:Goats; D006878:Hydroxides / Q000097:blood; D017665:Hydroxyl Radical; D007700:Kinetics; D015227:Lipid Peroxidation / Q000187:drug effects*; D008353:Mannitol / Q000494:pharmacology; D008565:Membrane Proteins / Q000097:blood / Q000302:isolation & purification; D013049:Spectrin / Q000378:metabolism*
D016166:Free Radical Scavengers; D005609:Free Radicals; D006878:Hydroxides; D008565:Membrane Proteins; D004921:Erythropoietin; D013049:Spectrin; D017665:Hydroxyl Radical; D000431:Ethanol; D008353:Mannitol; D003300:Copper; D017301:Ca(2+) Mg(2+)-ATPase; D001205:Ascorbic Acid