title: Tissue response to biphasic calcium phosphate ceramic with different ratios of HA/beta TCP in periodontal osseous defects.
abstract: The purpose of this study was to determine the optimal ratio of calcium hydroxyapatite (HA) to beta tricalcium phosphate (beta TCP) in a biphasic porous calcium phosphate (BCP) ceramic for effective repair of periodontal osseous defects. Defects were surgically produced in beagle dogs and made chronic for 4 months to simulate periodontal disease. Mucoperiosteal periodontal flaps were reflected, followed by osseous defect debridement and root planing. Specially prepared ceramic with different HA/beta TCP ratios were implanted into the prepared defects. The sites were allowed to heal for 6 months, animals were euthanized, and site-blocks were removed for histological study. During the follow-up phase, scaling and polishing were done once a month, and standardized probing attachment levels were recorded pre- and 6-months postoperatively. The Duncan's multiple range test showed that all the treatments produced statistically significant higher gain in probing attachment levels than the control group (0HA/0 beta TCP) (P < 0.05). Among the 7 "active" treatment groups, 2 (65/35 and 85/15) had significantly higher gain in probing attachment levels than those in 3 groups (50/50, 100/0, and 0/100) (P < 0.05). Histologically, higher HA ratio (but not 100% HA) showed accelerated new bone formation and new attachment levels. Based on histological results, the 85HA/15 beta TCP ratio appears to demonstrate greater gain in attachment level and bone regeneration in the treatment of periodontal osseous defects.
authors.affiliation: Clement J. Zablocki V.A. Medical Center, Dental Research Section, Milwaukee, WI.
authors.forename: E B
authors.initials: EB
authors.lastname: Nery
authors.forename: R Z
authors.initials: RZ
authors.lastname: LeGeros
authors.forename: K L
authors.initials: KL
authors.lastname: Lynch
authors.forename: K
authors.initials: K
authors.lastname: Lee
mesh_terms: D016301:Alveolar Bone Loss / Q000601:surgery*; D000539:Alveolar Process / Q000473:pathology; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D001861:Bone Regeneration; D002130:Calcium Phosphates / Q000032:analysis / Q000737:chemistry; D002516:Ceramics / Q000737:chemistry; D003094:Collagen; D003238:Connective Tissue / Q000473:pathology; D003739:Dental Cementum / Q000473:pathology; D015921:Dental Implants; D004285:Dogs; D017886:Durapatite; D004846:Epithelial Attachment / Q000473:pathology; D006882:Hydroxyapatites / Q000032:analysis / Q000737:chemistry; D012995:Solubility; D014092:Tooth Root / Q000473:pathology; D014945:Wound Healing
chemical_list: D001672:Biocompatible Materials; D002130:Calcium Phosphates; D015921:Dental Implants; D006882:Hydroxyapatites; C485817:beta-tricalcium phosphate; D003094:Collagen; D017886:Durapatite; C018392:tricalcium phosphate