title: [Pyrophosphate-dependent inactivation of tyrosyl-tRNA synthetase during aminoacylation of heterologous tRNA].
abstract: It has been shown that heterologous aminoacylation of tRNA by tyrosyl-tRNA synthetase leads to inactivation of the enzyme. Inorganic pyrophosphatase prevents the inactivation and increases the enzyme activity and aminoacylation level in a heterologous system. A putative inactivation mechanism is discussed.
authors.forename: A M
authors.initials: AM
authors.lastname: Khvorova
authors.forename: Iu A
authors.initials: IuA
authors.lastname: Motorin
authors.forename: A D
authors.initials: AD
authors.lastname: Vol'fson
mesh_terms: D000215:Acylation; D000588:Amines / Q000737:chemistry; D000818:Animals; D002417:Cattle; D011756:Diphosphates / Q000378:metabolism*; D008099:Liver / Q000201:enzymology; D012343:RNA, Transfer / Q000378:metabolism*; D011817:Rabbits; D014448:Tyrosine-tRNA Ligase / Q000037:antagonists & inhibitors* / Q000378:metabolism
chemical_list: D000588:Amines; D011756:Diphosphates; D012343:RNA, Transfer; D014448:Tyrosine-tRNA Ligase
vernacular_title: Pirofosfatzavisimaia inaktivatsiia tirozil-tRNK sintetazy pri aminoatsilirovanii geterologichnoĭ tRNK.