Activation of GABA B receptors in the anterior pituitary inhibits prolactin and luteinizing hormone secretion.
Previous work from our laboratory showed that baclofen could lower serum prolactin (PRL) levels acting at the central nervous system. The present experiments were designed to evaluate whether the gamma-aminobutyric acid B agonist was also effective in inhibiting hormone release at the pituitary level. In monolayer cultures of adenohypophyseal dispersed cells, baclofen inhibited basal PRL secretion after 1 or 2 h of incubation. This inhibition was significantly abolished by three antagonists: phaclofen, 3-aminopropyl-phosphonic acid and 4-aminobutylphosphonic acid. Furthermore, baclofen inhibited the thyrotropin-releasing hormone-induced PRL release in a concentration-dependent manner. With regard to gonadotropin secretion, baclofen was unable to modify basal luteinizing hormone (LH) secretion, but significantly inhibited the LH-releasing hormone-induced LH release. These results show that baclofen, in addition to its central neuroendocrine effects, inhibits pituitary hormone secretion, under basal and/or stimulated conditions, by direct action at the pituitary level.
Lux-Lantos
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V
Instituto de Biologia y Medicina Experimental, Buenos Aires, Argentina.
Rey
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E
Libertun
C
C
D000818:Animals; D001418:Baclofen / Q000031:analogs & derivatives / Q000494:pharmacology; D002082:Butylamines / Q000494:pharmacology; D002478:Cells, Cultured; D005260:Female; D018756:GABA Antagonists; D007987:Gonadotropin-Releasing Hormone / Q000494:pharmacology; D007774:Lactation / Q000502:physiology; D007986:Luteinizing Hormone / Q000378:metabolism*; D009943:Organophosphorus Compounds / Q000494:pharmacology; D010903:Pituitary Gland, Anterior / Q000187:drug effects / Q000502:physiology*; D011359:Proestrus / Q000502:physiology; D011388:Prolactin / Q000378:metabolism*; D011437:Propylamines / Q000494:pharmacology; D051381:Rats; D011963:Receptors, GABA-A / Q000187:drug effects / Q000502:physiology*; D013973:Thyrotropin-Releasing Hormone / Q000494:pharmacology
D002082:Butylamines; D018756:GABA Antagonists; D009943:Organophosphorus Compounds; D011437:Propylamines; D011963:Receptors, GABA-A; C052329:phaclofen; D007987:Gonadotropin-Releasing Hormone; C079360:4-aminobutylphosphonic acid; D013973:Thyrotropin-Releasing Hormone; D011388:Prolactin; D007986:Luteinizing Hormone; D001418:Baclofen; C011649:3-aminopropylphosphonic acid