Attachment of mitochondria to intermediate filaments in adrenal cells: relevance to the regulation of steroid synthesis.
The rate of steroid synthesis is regulated by the rate of transport of cholesterol from lipid droplets to mitochondria. We have previously demonstrated that lipid droplets in adrenal cells are tightly attached to intermediate filaments. Here we now show that mitochondria colocalize with intermediate filaments in modified double indirect immunofluorescence and by electron microscopy of extracted adrenal cells. Direct contact between mitochondria and intermediate filaments was established by examination of stereo pairs of electron micrographs from extracted cells. The attachment of both droplets and mitochondria to intermediate filaments suggests possible mechanisms for this form of intracellular transport of cholesterol to mitochondria and hence for the regulation of steroid synthesis.
Almahbobi
G
G
Department of Endocrinology, Prince of Wales Hospital, Randwick, New South Wales, Australia.
Williams
L J
LJ
Hall
P F
PF
D000311:Adrenal Glands / Q000378:metabolism / Q000648:ultrastructure*; D000818:Animals; D002417:Cattle; D002451:Cell Compartmentation; D002458:Cell Fractionation; D002478:Cells, Cultured; D002784:Cholesterol / Q000378:metabolism; D003576:Electron Transport Complex IV / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D066298:In Vitro Techniques; D007382:Intermediate Filaments / Q000378:metabolism* / Q000648:ultrastructure; D051379:Mice; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D008928:Mitochondria / Q000378:metabolism* / Q000648:ultrastructure; D013256:Steroids / Q000096:biosynthesis*
D013256:Steroids; D002784:Cholesterol; D003576:Electron Transport Complex IV