Elevated expression of annexin II (lipocortin II, p36) in a multidrug resistant small cell lung cancer cell line.
The doxorubicin-selected multidrug resistant small cell lung cancer cell line, H69AR, is cross-resistant to the Vinca alkaloids and epipodophyllotoxins, but does not overexpress P-glycoprotein, a 170 kDa plasma membrane efflux pump usually associated with this type of resistance. Monoclonal antibodies were raised against the H69AR cell line and one of these, MAb 3.186, recognises a peptide epitope on a 36 kDa phosphorylated protein that is membrane associated, but not presented on the external surface of H69AR cells (Mirski & Cole, 1991). In the present study, in vitro translation and molecular cloning techniques were used to determine the relative levels of mRNA corresponding to the 3.186 antigen. In addition, a cDNA clone containing an insert of approximately 1.4 kb was obtained by screening an H69AR cDNA library with 125I-MAb 3.186. Fragments of this cloned DNA hybridised to a single mRNA species of approximately 1.6 kb that was 5 to 6-fold elevated in H69AR cells. Partial DNA sequencing and restriction endonuclease mapping revealed identity of the cloned DNA with p36, a member of the annexin/lipocortin family of Ca2+ and phospholipid binding proteins.
Cole
S P
SP
Cancer Research Laboratories, Queen's University, Kingston, Ontario, Canada.
Pinkoski
M J
MJ
Bhardwaj
G
G
Deeley
R G
RG
D017302:Annexins; D000911:Antibodies, Monoclonal; D002135:Calcium-Binding Proteins / Q000378:metabolism*; D018288:Carcinoma, Small Cell / Q000378:metabolism*; D004247:DNA / Q000235:genetics; D004351:Drug Resistance; D015870:Gene Expression; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism*; D011233:Precipitin Tests; D012333:RNA, Messenger / Q000235:genetics; D015183:Restriction Mapping; D014407:Tumor Cells, Cultured
D017302:Annexins; D000911:Antibodies, Monoclonal; D002135:Calcium-Binding Proteins; D012333:RNA, Messenger; D004247:DNA