Uptake and incorporation of inositol by preimplantation mouse embryos.
The uptake of myo-inositol by preimplantation mouse embryos was investigated using [3H]myo-inositol. Uptake increased about 12-fold between one- and two-cell stages and increased again at the blastocyst stage (> 6-fold compared with the two-cell stage). Uptake at the blastocyst stage was time and temperature dependent; it was stimulated by sodium, inhibited by glucose and appeared to take place mainly via a saturable mechanism. Uptake in the presence of 6.25 mmol inositol l-1 was 1424 fmol inositol per blastocyst per h. About 10% of the [3H]inositol taken up by blastocysts during 8 h in culture was incorporated into lipid. Thin layer chromatography of the lipid showed that most of this inositol was incorporated into lipid material co-migrating with phosphatidylinositol with a small proportion co-migrating with phosphatidylinositol 4-phosphate.
Kane
M T
MT
Department of Molecular Embryology, AFRC Institute of Animal Physiology and Genetics Research, Barbraham, Cambridge, UK.
Norris
M
M
Harrison
R A
RA
D000704:Analysis of Variance; D000818:Animals; D001755:Blastocyst / Q000187:drug effects / Q000378:metabolism*; D002855:Chromatography, Thin Layer; D002970:Cleavage Stage, Ovum / Q000378:metabolism; D003080:Cold Temperature / Q000009:adverse effects; D003470:Culture Media; D004305:Dose-Response Relationship, Drug; D005947:Glucose / Q000494:pharmacology; D007294:Inositol / Q000493:pharmacokinetics*; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D010716:Phosphatidylinositols / Q000378:metabolism; D012964:Sodium / Q000494:pharmacology
D003470:Culture Media; D010716:Phosphatidylinositols; D007294:Inositol; D012964:Sodium; D005947:Glucose