Preliminary results towards ciguatoxin immunodetection.
Due to the lack of purified ciguatoxin (CTX), monensin was used as a model for developing an enzyme immunoassay to detect CTX. Specific antibodies directed against monensin have been produced in rabbits and mice using a monensin-protein immunogen obtained in bulk quantities. Rabbit polyclonal and mouse monoclonal (MAb) antibodies of high specificity and affinity have been produced. Using MAb 2H8, in a competitive micro-ELISA performed in Terasaki plates, the detection limit for free monensin was 75 pg. No cross-reactivity was detected against CTX but a procedure requiring only 100 micrograms of hapten is under current investigation with a brevetoxin (PbTx-3), another marine toxin with a polyether backbone structure similar to CTX and recently commercially available.
Pauillac
S
S
Institut Pasteur de Paris, Unité d'Immunocytochimie, France.
Labrousse
H
H
Legrand
A M
AM
Avrameas
S
S
D000818:Animals; D000906:Antibodies / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology; D000915:Antibody Affinity; D000918:Antibody Specificity; D000941:Antigens / Q000276:immunology; D002922:Ciguatoxins / Q000032:analysis*; D005260:Female; D007124:Immunoenzyme Techniques; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008985:Monensin / Q000276:immunology; D010047:Ovalbumin / Q000276:immunology; D011817:Rabbits; D012710:Serum Albumin, Bovine / Q000276:immunology
D000906:Antibodies; D000911:Antibodies, Monoclonal; D000941:Antigens; D002922:Ciguatoxins; D012710:Serum Albumin, Bovine; D010047:Ovalbumin; D008985:Monensin