title: Transcriptional regulation of transferrin and albumin genes by retinoic acid in human hepatoma cell line Hep3B.
abstract: Transferrin and albumin, which are both secreted from the human hepatoma cell line Hep3B, were regulated transcriptionally by retinoic acid (RA) in a dose-dependent manner. The cell growth rate was little affected under the same conditions. The treatment of Hep3B cells with RA (10 microM for 48 h) resulted in an 8-fold increase in transferrin protein synthesis, a 10-fold increase in the steady-state transferrin mRNA level, and a 5-fold increase in its transcriptional rate. The same treatment led to 4-fold decrease in albumin synthesis, as well as a 7-fold decline in the steady-state albumin mRNA level and a 4-fold decrease in the transcriptional rate. Cycloheximide and actinomycin D blocked the action of RA, suggesting that RA may regulate transferrin and albumin gene expression indirectly in human liver cells.
authors.affiliation: Graduate Institute of Microbiology and Immunology, National Yang-Ming Medical College, Taipei, Taiwan, Republic of China.
authors.forename: S L
authors.initials: SL
authors.lastname: Hsu
authors.forename: Y F
authors.initials: YF
authors.lastname: Lin
authors.forename: C K
authors.initials: CK
authors.lastname: Chou
mesh_terms: D015152:Blotting, Northern; D006528:Carcinoma, Hepatocellular; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002467:Cell Nucleus / Q000187:drug effects / Q000502:physiology*; D003513:Cycloheximide / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D007700:Kinetics; D008113:Liver Neoplasms; D012334:RNA, Neoplasm / Q000235:genetics / Q000302:isolation & purification; D012709:Serum Albumin / Q000235:genetics*; D014158:Transcription, Genetic / Q000187:drug effects; D014168:Transferrin / Q000235:genetics*; D014212:Tretinoin / Q000494:pharmacology*
chemical_list: D012334:RNA, Neoplasm; D012709:Serum Albumin; D014168:Transferrin; D003609:Dactinomycin; D014212:Tretinoin; D003513:Cycloheximide