Identification and functional analysis of the fowlpox virus homolog of the vaccinia virus p37K major envelope antigen gene.
A fowlpox virus (FPV) gene with homology to the vaccinia virus p37K major envelope antigen gene was identified and sequenced. The predicted product has a molecular weight of 43,018 Da (p43K). The FPV p43K gene has 37.5% identity with its vaccinia counterpart and higher homology with a molluscum contagiosum virus gene (42.6% identity). Based on upstream sequences, p43K appears to be regulated as a late gene. Recombinant FPV were generated in which a large portion of p43K was replaced by the Escherichia coli lacZ gene. These recombinants failed to produce visible plaques under standard conditions. After prolonged incubation the microplaques developed into small macroscopic plaques. Plaques were purified on the basis of lacZ expression. Single-cycle growth curves comparing the p43K-deleted recombinant (designated fJd43Z) with parental FPV showed that the two viruses produce identical amounts of intracellular virions, but that fJd43Z released 20-fold fewer infectious particles into the medium. CsCl gradient centrifugation of [3H]thymidine-labeled virus was employed to examine differences in the production of physical particles. The two viruses produced equivalent levels of intracellular virions, but fJd43Z failed to produce detectable levels of released particles. FPV p43K is therefore involved in the release of virions from infected cells.
Calvert
J G
JG
USDA Agricultural Research Service, Avian Disease and Oncology Laboratory, East Lansing, Michigan 48823.
Ogawa
R
R
Yanagida
N
N
Nazerian
K
K
D000595:Amino Acid Sequence; D001483:Base Sequence; D002500:Centrifugation, Isopycnic; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D005587:Fowlpox virus / Q000235:genetics* / Q000254:growth & development; D015967:Gene Expression Regulation, Viral; D005814:Genes, Viral / Q000235:genetics*; D007763:Lac Operon; D008969:Molecular Sequence Data; D016296:Mutagenesis; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification; D016415:Sequence Alignment; D014616:Vaccinia virus / Q000235:genetics; D010948:Viral Plaque Assay; D014764:Viral Proteins / Q000235:genetics* / Q000302:isolation & purification; D014771:Virion / Q000254:growth & development
D011994:Recombinant Proteins; D014764:Viral Proteins; C078427:p43K protein, Fowlpox virus