title: Different early effect of irradiation in brain and small cell lung cancer examined by in vivo 31P-magnetic resonance spectroscopy.
abstract: Early effects of irradiation were evaluated by non-invasive in vivo 31P-magnetic resonance spectroscopy (31P-MRS) of two small cell lung cancer (SCLC) tumor lines CPH SCCL 54A and 54B, in nude mice. The tumors were originally derived from the same patient and have similar morphology and growth characteristics, but a different radiosensitivity. The 54A tumors are twice as radiosensitive as the 54B's. In the present study the tumors were treated with 2.5, 10, and 40 Gy. For comparison, nude mice were given cranial irradiation at the same three doses, and the effect was evaluated by in vivo 31P-MRS. No effect was observed in brain at any dose level. In contrast, 40 Gy induced a statistically significant reduction in ATP/Pi ratio during the 12-h post-irradiation period. This effect was more pronounced in 54A than in 54B. Some reduction was observed following 10 Gy, whereas 2.5 Gy induced no changes in ATP/Pi. The differential effect on tumors and brain might be relevant for monitoring irradiation effects by in vivo 31P-MRS in patients with brain metastases.
authors.affiliation: Department of Oncology, Finsen Institute, Blegdamsvej, Copenhagen, Denmark.
authors.forename: P E
authors.initials: PE
authors.lastname: Kristjansen
authors.forename: A G
authors.initials: AG
authors.lastname: Pedersen
authors.forename: B
authors.initials: B
authors.lastname: Quistorff
authors.forename: M
authors.initials: M
authors.lastname: Spang-Thomsen
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism / Q000528:radiation effects*; D018288:Carcinoma, Small Cell / Q000378:metabolism / Q000532:radiotherapy*; D004307:Dose-Response Relationship, Radiation; D008175:Lung Neoplasms / Q000378:metabolism / Q000532:radiotherapy*; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D011836:Radiation Tolerance; D014407:Tumor Cells, Cultured