title: Inhibition and affinity chromatography of chicken lung angiotensin I-converting enzyme with captopril.
abstract: 1. Angiotensin I-converting enzyme (EC 3.4.15.1) has been purified to electrophoretic homogeneity from chicken lung by using a facile two-step protocol which included affinity chromatography on Sepharose-bound captopril. 2. Captopril was a potent inhibitor of chicken lung angiotensin I-converting enzyme with Ki values of 2.0 nmol/l and 1.6 nmol/l for detergent-extracted and trypsin-extracted angiotensin I-converting enzymes, respectively. 3. Molecular weight comparison of trypsin-extracted (M(r)270,000) and detergent-extracted (M(r)690,000) angiotensin I-converting enzyme indicated that membrane-binding sequence contributed to a large extent to the enzyme molecule. 4. Kinetic properties of both forms of the enzyme suggested that the membrane-bound sequence contributed to an increase of the enzyme-substrate affinity.
authors.affiliation: Departamento de Bioquímica, Biología Molecular y Fisiologia, Facultad de Ciencias, Universidad de Valladolid, Spain.
authors.forename: M J
authors.initials: MJ
authors.lastname: Polanco
authors.forename: M T
authors.initials: MT
authors.lastname: Agapito
authors.forename: J M
authors.initials: JM
authors.lastname: Recio
mesh_terms: D000818:Animals; D002216:Captopril / Q000494:pharmacology; D002645:Chickens; D002846:Chromatography, Affinity; D066298:In Vitro Techniques; D007700:Kinetics; D008168:Lung / Q000201:enzymology*; D008970:Molecular Weight; D007703:Peptidyl-Dipeptidase A / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D002216:Captopril; D007703:Peptidyl-Dipeptidase A