Analysis of the promoter of the cytochrome P-450 2B2 gene in the rat.
About 3 kb of the promoter region of the gene encoding cytochrome P-450 2B2 (CYP2B2) in the rat were sequenced and searched for potential cis-acting elements. Apart from putative binding sites for (liver-specific) protein factors, a region showing homology with the LINE 1 retrotransposon element was also found. Three proximal promoter fragments, encompassing nucleotides -579 to -372, -372 to -211, and -211 to +1, respectively, were shown to contain binding sites for multiple protein factors by bandshift analyses. The strongest protein-binding element, designated BRE (basic regulatory element), occurs between -103 to -66. Its structure is very similar to a negative control element in the murine cmyc promoter and displays a composite feature having a tandemly repeated sequence homology with the BTE (basic transcription element; Yanagida et al., 1990) separated by a CCAAA-box. The use of a deletion series of this template in in vitro transcription assays, provided evidence that the BRE serves as a major cis-acting element in the (regulated) transcription activation of the CYP2B2 gene.
Hoffmann
M
M
Department of Biochemistry and Molecular Biology, Vrije Universiteit, Amsterdam, The Netherlands.
Mager
W H
WH
Scholte
B J
BJ
Civil
A
A
Planta
R J
RJ
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D004251:DNA Transposable Elements; D004790:Enzyme Induction; D005796:Genes; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D051381:Rats; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic
D004251:DNA Transposable Elements; D003577:Cytochrome P-450 Enzyme System