Serum immunoassay of a small cell lung cancer associated ganglioside: development of a sensitive scintillation proximity assay.
We here report an enzyme linked immunosorbent assay (ELISA) and a scintillation proximity assay (SPA) for detection of the ganglioside FucGM1 in sera from small cell lung cancer (SCLC) patients. The SPA was more sensitive and reproducible than the ELISA. In this assay, monoclonal antibodies specific for FucGM1 were bound to SPA particles and incubated with labelled FucGM1 and 100 microliters test-serum overnight, and counted in a beta-counter. The sensitivity was 0.2 ng. Seven out of twenty sera from SCLC patients were positive, whereas none of twenty sera from healthy individuals were positive for FucGM1. The SPA was more sensitive than the previously reported HPTLC as well as a direct ELISA.
Pallesen
T
T
Fibiger Institute, Department of Tumor Cell Biology, Copenhagen, Denmark.
Vangsted
A
A
Drivsholm
L
L
Clausen
H
H
Zeuthen
J
J
Wallin
H
H
D002240:Carbohydrate Sequence; D018288:Carcinoma, Small Cell / Q000097:blood* / Q000175:diagnosis; D004797:Enzyme-Linked Immunosorbent Assay; D005677:G(M1) Ganglioside / Q000031:analogs & derivatives* / Q000097:blood; D006801:Humans; D006825:Hybridomas; D007118:Immunoassay; D008175:Lung Neoplasms / Q000097:blood* / Q000175:diagnosis; D008969:Molecular Sequence Data; D011379:Prognosis; D012588:Scintillation Counting; D012680:Sensitivity and Specificity; D014407:Tumor Cells, Cultured
D005677:G(M1) Ganglioside; C039123:fucosyl GM1 ganglioside