[The effect of carnosine on Ca-channels in rabbit skeletal muscle sarcoplasmic reticulum].
Carnosine (beta-alanyl-L-histidine), which is present in millimolar concentrations in skeletal muscles, induces Ca2+ release from the heavy fraction of rabbit skeletal muscle sarcoplasmic reticulum by activation ruthenium red-sensitive Ca-release channels. The effect of carnosine is dose-dependent, which indicates the presence of saturable carnosine-binding sites in the Ca-release channel molecule. The half-maximal Ca2+ release is observed in the presence of 8.7 mM carnosine. At the same time, carnosine addition to the medium increases the affinity of sarcoplasmic reticulum Ca-channels for the Ca-release activators, caffeine and adenine nucleotides. It is concluded that carnosine is an endogenous regulator of skeletal muscle sarcoplasmic reticulum Ca-channels which modulates the affinity of these channels for different ligands.
Batrukova
M A
MA
Rubtsov
A M
AM
Boldyrev
A A
AA
D000227:Adenine Nucleotides / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002110:Caffeine / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D015220:Calcium Channels / Q000187:drug effects*; D002336:Carnosine / Q000494:pharmacology*; D002413:Cations, Divalent; D009132:Muscles / Q000648:ultrastructure*; D011817:Rabbits; D012519:Sarcoplasmic Reticulum / Q000187:drug effects*; D013379:Substrate Specificity
D000227:Adenine Nucleotides; D015220:Calcium Channels; D002413:Cations, Divalent; D002110:Caffeine; D002336:Carnosine; D000255:Adenosine Triphosphate; D002118:Calcium
Vlianie karnozina na Ca-kanaly sarkoplazmaticheskogo retikuluma skeletnykh myshts.