Differential effect in vitro of tumor necrosis factor-alpha (TNF) on normal and virus-infected erythroid progenitors from Friend virus (FVA)-infected mice.
In vivo administration of tumor necrosis factor-alpha (TNF) suppresses both normal and Friend virus (FVA)-infected erythroid progenitor cells (CFU-E). To examine the mechanism of erythroid suppression by TNF, we examined TNF's direct effect on normal and virus-infected cells in vitro. Productively infected fibroblast cell lines, fresh acute virus-infected spleen cells, and virus-infected CFU-E were sensitive, whereas uninfected CFU-E were resistant to TNF cytotoxicity in vitro. When FVA-infected erythroblasts were depleted from the spleen cell population in vitro with antivirus antibodies, TNF suppression of the remaining (uninfected) cells was abrogated. In contrast, both normal and virus-infected macrophage progenitor cells and immature erythroid progenitor cells were equally sensitive to TNF cytotoxicity in vitro. Normal erythroblasts had significantly fewer TNF receptors than FVA-infected erythroblasts, which also were morphologically less mature. These results suggest that TNF can differentially suppress late-stage virus-infected erythroid progenitors in vitro.
Chang
M J
MJ
Department of Otolaryngology, University of Pittsburgh, School of Medicine, Pennsylvania 15213.
Pourbohloul
S C
SC
Yu
W D
WD
Furmanski
P
P
Johnson
C S
CS
D000818:Animals; D016923:Cell Death; D002478:Cells, Cultured; D003609:Dactinomycin / Q000494:pharmacology; D015672:Erythroid Precursor Cells / Q000187:drug effects / Q000382:microbiology / Q000473:pathology*; D005622:Friend murine leukemia virus; D007106:Immune Sera; D004915:Leukemia, Erythroblastic, Acute / Q000473:pathology*; D051379:Mice; D008815:Mice, Inbred Strains; D010659:Phenylhydrazines / Q000494:pharmacology; D011956:Receptors, Cell Surface / Q000378:metabolism; D018124:Receptors, Tumor Necrosis Factor; D011994:Recombinant Proteins / Q000494:pharmacology; D015682:Retroviridae Proteins, Oncogenic / Q000276:immunology; D013154:Spleen / Q000473:pathology; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*; D014759:Viral Envelope Proteins / Q000276:immunology
D007106:Immune Sera; D010659:Phenylhydrazines; D011956:Receptors, Cell Surface; D018124:Receptors, Tumor Necrosis Factor; D011994:Recombinant Proteins; D015682:Retroviridae Proteins, Oncogenic; D014409:Tumor Necrosis Factor-alpha; D014759:Viral Envelope Proteins; C030299:phenylhydrazine; D003609:Dactinomycin