A new surfactant series, the N-alkylamino-1-deoxylactitols: application for extraction of 'op' opiate receptors from frog brain.
A new series of surfactants, the N-alkylamino-1-deoxylactitols, was prepared and employed to extract 'op' opiate receptors from frog brain. These surfactants are both cheap and convenient to prepare. Receptors were reproducibly extracted in a good yield using N-nonylamino-1-deoxylactitol. This derivative, which was not denaturing during the extraction process, could thus be used instead of the more costly digitonin, whose rather variable purity affects yield.
Garelli-Calvet
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R
Laboratoire des IMRCP, UA CNRS No. 470, Université Paul Sabatier, Toulouse, France.
Latgé
P
P
Rico
I
I
Lattes
A
A
Puget
A
A
D000605:Amino Alcohols / Q000138:chemical synthesis / Q000737:chemistry; D000818:Animals; D001923:Brain Chemistry; D004072:Digitonin; D011895:Rana ridibunda; D011957:Receptors, Opioid / Q000302:isolation & purification*; D013501:Surface-Active Agents; D014020:Tissue Extracts
D000605:Amino Alcohols; D011957:Receptors, Opioid; D013501:Surface-Active Agents; D014020:Tissue Extracts; D004072:Digitonin