Cloning and analysis of a Candida maltosa gene which confers resistance to formaldehyde in Saccharomyces cerevisiae.
A gene (FDH1) of Candida maltosa which confers resistance to formaldehyde in Saccharomyces cerevisiae was cloned and its nucleotide sequence determined. The gene has a single intron which possesses the highly conserved splicing signals found in S. cerevisiae introns. We demonstrated that processing of the pre-mRNA of the cloned gene occurred identically in both S. cerevisiae and C. maltosa. The predicted amino acid sequence from the cloned gene showed 65.5% identity to human alcohol dehydrogenase (ADH) class III and 23.9% identity to S. cerevisiae ADH1. The most probable mechanism of resistance to formaldehyde is thought to be the glutathione-dependent oxidation of formaldehyde which is characteristic for ADH class III. The cloned FDH1 gene was successfully employed as a dominant selectable marker in the transformation of S. cerevisiae.
Sasnauskas
K
K
Department of Molecular Biology, Institute of Biotechnology, Vilnius, Lithuania.
Jomantiene
R
R
Januska
A
A
Lebediene
E
E
Lebedys
J
J
Janulaitis
A
A
D000426:Alcohol Dehydrogenase / Q000235:genetics; D000595:Amino Acid Sequence; D001483:Base Sequence; D002175:Candida / Q000235:genetics*; D003001:Cloning, Molecular; D004271:DNA, Fungal; D004352:Drug Resistance, Microbial / Q000235:genetics; D005091:Exons; D005557:Formaldehyde / Q000494:pharmacology*; D005560:Formate Dehydrogenases; D005656:Fungal Proteins / Q000235:genetics*; D005800:Genes, Fungal; D006801:Humans; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
D004271:DNA, Fungal; C499417:FDH1 protein, Candida; D005656:Fungal Proteins; D005557:Formaldehyde; D000426:Alcohol Dehydrogenase; D005560:Formate Dehydrogenases