Adrenergic inhibition of insulin secretion involves pertussis toxin-sensitive and -insensitive mechanisms.
We studied the involvement of Bordetella pertussis toxin (PTX)-sensitive G proteins in the inhibition by adrenaline of insulin secretion from the isolated rat pancreas. The -90% inhibition induced by adrenaline (0.05 microM) was partially abolished after in vivo PTX pretreatment. The residual inhibitory effect of adrenaline in PTX-pretreated rats was suppressed by the alpha 2-adrenoceptor antagonist, yohimbine, but was not modified by the alpha 1-adrenoceptor antagonist, prazosin. Thus, the alpha 2-inhibitory effect of adrenaline on B-cells is mediated by both PTX-sensitive and PTX-insensitive mechanisms.
Hillaire-Buys
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Faculté de Médecine, Laboratorie de Pharmacologie, Centre National de la Recherche Scientifique, Montpellier, France.
Gross
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Roye
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Ribes
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Loubatières-Mariani
M M
MM
D000704:Analysis of Variance; D000818:Animals; D004837:Epinephrine / Q000494:pharmacology; D019204:GTP-Binding Proteins / Q000187:drug effects*; D005934:Glucagon / Q000378:metabolism; D066298:In Vitro Techniques; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D037342:Pertussis Toxin; D011224:Prazosin / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D011942:Receptors, Adrenergic, alpha / Q000187:drug effects / Q000502:physiology*; D010566:Virulence Factors, Bordetella / Q000494:pharmacology*; D015016:Yohimbine / Q000494:pharmacology
D007328:Insulin; D011942:Receptors, Adrenergic, alpha; D010566:Virulence Factors, Bordetella; D015016:Yohimbine; D005934:Glucagon; D037342:Pertussis Toxin; D019204:GTP-Binding Proteins; D011224:Prazosin; D004837:Epinephrine