Parathyroid hormone regulation of matrix degrading enzymes in rat osteoblastic osteosarcoma 17/2.8 cells.
The present study was designed to further understand the role of PTH on the secretion of the neutral metalloproteinases, collagenase and gelatinase, from the rat osteosarcoma clonal cell line, ROS 17/2.8. Semiconfluent cells were treated with bovine parathyroid hormone, b-PTH-(1-34) at 100 nM-0.01 nM for 24-96 hours and pooled, concentrated media were analyzed by functional assay for collagenase (3H-methyl collagen) and gelatinase (3H-methyl gelatin). Collagenase activity significantly decreased (P less than 0.01) in the PTH conditioned media in a dose-dependent manner before (98-64%) and after (91-39%) reduction and alkylation. SDS-PAGE and fluorography apparently showed the most degradation to alpha A chains in collagen with controls, whereas this substrate remained intact with PTH (100 nM). PTH (100 nM) media also showed neutral gelatinase activity approximately 2% compared to control before and after reduction and alkylation (P less than 0.01). Significant amounts of an inhibitor to collagenase and gelatinase might have been secreted at 1 nM and 0.01 nM PTH, since collagenase and gelatinase activities were greater after reduction and alkylation. Reduction and alkylation likely destroyed these significant amounts of inhibitor. Polymorphonuclear leukocyte collagenase activity was also inhibited 80% by PTH conditioned media, but not by control. However, upon reduction and alkylation which destroyed inhibitor, the PTH treated media showed only a 14% inhibition against polymorphonuclear leukocyte collagenase (P less than 0.01). PTH appeared to downregulate neutral metalloproteinase activities through its effects on an inhibitor. This downregulation may represent a specific phenotypic response to PTH in ROS 17/2.8 cells.
Vernillo
A T
AT
Department of Oral Medicine, New York University College of Dentistry, N.Y. 10010.
Ramamurthy
N S
NS
Lee
H M
HM
Rifkin
B R
BR
D000478:Alkylation; D000818:Animals; D002417:Cattle; D003470:Culture Media; D018093:Gelatinases; D006023:Glycoproteins / Q000494:pharmacology; D006801:Humans; D003012:Microbial Collagenase / Q000037:antagonists & inhibitors / Q000097:blood / Q000378:metabolism; D009504:Neutrophils / Q000201:enzymology; D010006:Osteoblasts / Q000201:enzymology / Q000473:pathology; D012516:Osteosarcoma / Q000201:enzymology* / Q000378:metabolism; D010084:Oxidation-Reduction; D010281:Parathyroid Hormone / Q000494:pharmacology*; D010434:Pepsin A / Q000037:antagonists & inhibitors / Q000097:blood / Q000378:metabolism; D051381:Rats; D019714:Tissue Inhibitor of Metalloproteinases; D014407:Tumor Cells, Cultured / Q000187:drug effects
D003470:Culture Media; D006023:Glycoproteins; D010281:Parathyroid Hormone; D019714:Tissue Inhibitor of Metalloproteinases; D010434:Pepsin A; D018093:Gelatinases; D003012:Microbial Collagenase