title: cAMP production via the adenylyl cyclase pathway is reduced in RCS rat RPE.
abstract: cAMP production was investigated in retinal pigment epithelium (RPE) cells isolated from normal rats and from rats with an inherited retinal dystrophy (Rdy/p+). In normal RPE cells, 5'-[N-Ethylcarboxamido]-adenosine (A2 receptors) produced a fivefold increase in the level of cyclic adenosine monophosphate (cAMP) over basal levels. However, only a onefold increase in cAMP was observed in dystrophic cells. cAMP production by prostaglandins E1 and E2 (prostaglandin receptors) in dystrophic RPE cells was only 29-38% of the level observed in normal cells. Direct stimulation of adenylyl cyclase by 10 mumol/l forskolin increased cAMP levels in normal RPE cells by 90 fold over basal, but only by sixfold in the dystrophic cells. These data suggest there may be a defect in the adenylyl cyclase signaling pathway in dystrophic RPE cells.
authors.affiliation: Jules Stein Eye Institute, UCLA School of Medicine 90024-7008.
authors.forename: C Y
authors.initials: CY
authors.lastname: Gregory
authors.forename: T A
authors.initials: TA
authors.lastname: Abrams
authors.forename: M O
authors.initials: MO
authors.lastname: Hall
mesh_terms: D000241:Adenosine / Q000031:analogs & derivatives / Q000494:pharmacology; D019830:Adenosine-5'-(N-ethylcarboxamide); D000262:Adenylyl Cyclases / Q000378:metabolism*; D000818:Animals; D005576:Colforsin / Q000494:pharmacology; D000242:Cyclic AMP / Q000096:biosynthesis*; D004195:Disease Models, Animal; D010857:Pigment Epithelium of Eye / Q000187:drug effects / Q000378:metabolism*; D011458:Prostaglandins E / Q000494:pharmacology; D051381:Rats; D011922:Rats, Mutant Strains; D011982:Receptors, Prostaglandin / Q000378:metabolism; D011983:Receptors, Purinergic / Q000378:metabolism; D012162:Retinal Degeneration / Q000378:metabolism*; D015398:Signal Transduction; D014665:Vasodilator Agents / Q000494:pharmacology
chemical_list: D011458:Prostaglandins E; D011982:Receptors, Prostaglandin; D011983:Receptors, Purinergic; D014665:Vasodilator Agents; D005576:Colforsin; D019830:Adenosine-5'-(N-ethylcarboxamide); D000242:Cyclic AMP; D000262:Adenylyl Cyclases; D000241:Adenosine