Response of colostrum-deprived cynomolgus monkeys to intragastric challenge exposure with simian rotavirus strain SA11.
The infectivity and pathogenic potential of a cell culture-adapted simian rotavirus was evaluated in colostrum-deprived newborn and infant cynomolgus macaques (Macaca fascicularis). Intragastric challenge exposure with the simian rotavirus strain SA11 on postpartum day 2 induced diarrhea in 5 of 5 colostrum-deprived newborn monkeys. Compared with sham-inoculated controls, 3 of the 5 inoculated monkeys also manifested reduced body weight gain during the initial 5 days after challenge exposure. Rotavirus was detected in feces of 3 challenge-exposed monkeys for up to 2 days after inoculation. Evaluation of antibody response after rotavirus inoculation was obscured by high but variable prechallenge-exposure serum titers of rotavirus-specific antibody. Preexisting serum titer of neutralizing antibody in newborn monkeys was not predictive of clinical response to inoculation with rotavirus SA11. Two 90-day-old infant monkeys with low serum neutralizing antibody titer did not have diarrhea, reduced weight gain, or antibody response after oral inoculation with rotavirus SA11. Results of these challenge-exposure studies in newborn cynomolgus monkeys were consistent with a heterologous host-rotavirus model and indicate that neonatal serum antibody of maternal origin may not be associated with resistance to rotavirus-induced disease.
Petschow
B W
BW
Mead Johnson Research Center, Bristol-Myers Squibb Co, Evansville, IN 47721.
Litov
R E
RE
Young
L J
LJ
McGraw
T P
TP
D000818:Animals; D000831:Animals, Newborn; D000914:Antibodies, Viral / Q000097:blood; D000956:Antigens, Viral / Q000032:analysis; D003967:Diarrhea / Q000276:immunology / Q000662:veterinary*; D004195:Disease Models, Animal; D004198:Disease Susceptibility; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000382:microbiology; D005312:Fetal Blood / Q000276:immunology; D007112:Immunity, Maternally-Acquired; D007124:Immunoenzyme Techniques; D008252:Macaca fascicularis; D008992:Monkey Diseases / Q000276:immunology*; D009500:Neutralization Tests; D012401:Rotavirus / Q000276:immunology / Q000302:isolation & purification; D012400:Rotavirus Infections / Q000276:immunology / Q000662:veterinary*; D015430:Weight Gain
D000914:Antibodies, Viral; D000956:Antigens, Viral