title: Inhibitory action of orotate, 2-thioorotate and isoorotate on nucleotide metabolism and nucleic acid synthesis in hepatoma cells.
abstract: 1. The specificity of the action of orotate on hepatoma cells was investigated. 2. Orotic acid and its methyl ester had similar inhibitory effects on the incorporation of [3H]thymidine into DNA of hepatoma cells. 3. In contrast to previous studies in vivo, incubation of rat kidney cells with orotate caused an increase in the ratio of UTP/ATP concentrations that was similar to effects on hepatic cells. 4. Inhibitory effects of 2-thioorotate and isoorotate on metabolism were found to be less selective and required higher concentrations than with orotate.
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Medicine and Dentistry of New Jersey, New Jersey Medical School, Newark 07103.
authors.forename: M A
authors.initials: MA
authors.lastname: Lea
authors.forename: A
authors.initials: A
authors.lastname: Luke
authors.forename: A
authors.initials: A
authors.lastname: Assad
authors.forename: M
authors.initials: M
authors.lastname: Patel
authors.forename: P A
authors.initials: PA
authors.lastname: Reddy
mesh_terms: D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000378:metabolism*; D002460:Cell Line; D004273:DNA, Neoplasm / Q000096:biosynthesis*; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism*; D009711:Nucleotides / Q000378:metabolism*; D009963:Orotic Acid / Q000031:analogs & derivatives / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014407:Tumor Cells, Cultured
chemical_list: D004273:DNA, Neoplasm; D009711:Nucleotides; D009963:Orotic Acid