Polymerized albumin receptor on rat liver cells.
The polymerized albumin hypothesis was proposed for the mechanism of a hepatitis B virus (HBV) infection of human liver parenchymal cells on the basis that a receptor for polymerized albumin treated with glutaraldehyde was detected on isolated human liver parenchymal cells. However, some controversy exists regarding this hypothesis, because a receptor for formaldehyde-treated bovine serum albumin (f-BSA) has been found on liver non-parenchymal cells. Therefore, we characterized the uptake of polymerized rat serum albumin (p-RSA) and f-BSA by rat liver in vivo, and their bindings to liver cells in vitro. Most p-RSA and f-BSA was taken up by the liver after intravenous administration, and the uptake of p-RSA was inhibited by a 1,000-fold excess of f-BSA. In addition, more than 80% of p-RSA taken up by the liver was found in the non-parenchymal cells, and the remainder was found in the parenchymal cells. P-RSA as well as f-BSA could bind to isolated rat liver parenchymal and non-parenchymal cells. Furthermore, p-RSA and f-BSA could bind to isolated rat liver cell plasma membranes, and these bindings were completely inhibited by 1,000-fold excess of either f-BSA or p-RSA. These results indicate that there is a receptor, which can recognize both p-RSA and f-BSA, on not only rat liver non-parenchymal cells but also the parenchymal cells. It is also indicated that the receptor on the parenchymal cells as well as the non-parenchymal cells is involved in the in vivo uptake of p-RSA.(ABSTRACT TRUNCATED AT 250 WORDS)
Takami
M
M
Division of Biochemical Genetics, Meiji Institute of Health Science, Kanagawa.
Kasuya
I
I
Tsunoo
H
H
D000818:Animals; D002417:Cattle; D002462:Cell Membrane / Q000378:metabolism; D066298:In Vitro Techniques; D007275:Injections, Intravenous; D008099:Liver / Q000378:metabolism*; D008970:Molecular Weight; D051381:Rats; D017457:Receptors, Albumin; D011956:Receptors, Cell Surface / Q000378:metabolism*; D012709:Serum Albumin / Q000008:administration & dosage / Q000302:isolation & purification / Q000378:metabolism*; D012710:Serum Albumin, Bovine / Q000378:metabolism; D000075462:Serum Albumin, Human; D014018:Tissue Distribution
D017457:Receptors, Albumin; D011956:Receptors, Cell Surface; D012709:Serum Albumin; C032137:polyalbumin; D012710:Serum Albumin, Bovine; D000075462:Serum Albumin, Human