[Susceptibility of RD-9H8 cells derived from RD cells to enteroviruses and its application for measuring neutralizing antibodies against coxsackievirus group B].
We carried out cloning of RD cells and obtained cloned cell line RD-9H8, which is highly sensitive to enteroviruses. Cytopathic effect (CPE) of RD-9H8 cells was more remarkable than that of original RD cells. The RD-9H8 cells were used to measure the neutralizing antibodies to coxsackievirus group B in sera from 93 medical students collected in Oct. 1990 at Iwate Medical University. The proportions of confirmed positive sera were coxsackievirus type B1 44.1%, type B2 50.5%, type B3 46.2%, type B4 64.5%, type B5 53.8% and type B6 4.4%.
Yanagihara
T
T
Department of Bacteriology, School of Medicine, Iwate Medical University.
Matsumoto
I
I
Kawana
R
R
D000914:Antibodies, Viral / Q000032:analysis*; D002999:Clone Cells; D003588:Cytopathogenic Effect, Viral; D004770:Enterovirus / Q000254:growth & development*; D029822:Enterovirus B, Human / Q000276:immunology*; D012208:Rhabdomyosarcoma; D014407:Tumor Cells, Cultured / Q000382:microbiology*
D000914:Antibodies, Viral