Generation of macaque B lymphoblastoid cell lines with simian Epstein-Barr-like viruses: transformation procedure, characterization of the cell lines and occurrence of simian foamy virus.
Two simian Epstein-Barr-like viruses, a rhesus Epstein-Barr virus and Herpesvirus papio, were used to transform B cells from rhesus or cynomolgus macaques. The resulting cell lines exhibited predominantly a B lymphocyte phenotype and expressed Epstein-Barr virus antigens. The majority of B lymphoblastoid cell lines from macaques, which were seropositive for simian foamy virus, developed giant cells in culture. The cytopathic agent was identified as a foamy virus and was transmissible to human embryonal fibroblasts. Treatment of cell cultures with AZT abolished giant cell formation.
Voss
G
G
Deutsches Primatenzentrum, Abteilung Virologie und Immunologie, Göttingen, Germany.
Nick
S
S
Stahl-Hennig
C
C
Ritter
K
K
Hunsmann
G
G
D000818:Animals; D000956:Antigens, Viral / Q000302:isolation & purification; D001402:B-Lymphocytes / Q000382:microbiology; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral; D003588:Cytopathogenic Effect, Viral / Q000187:drug effects; D015726:Giant Cells / Q000382:microbiology; D006564:Herpesviridae; D004854:Herpesvirus 4, Human / Q000276:immunology / Q000472:pathogenicity; D008251:Macaca; D016092:Spumavirus / Q000276:immunology / Q000472:pathogenicity; D015215:Zidovudine / Q000494:pharmacology
D000956:Antigens, Viral; D015215:Zidovudine