The source of non-heme iron that binds nitric oxide in cultivated macrophages.
In cultured macrophages (J 774 line) a decrease in iron-sulfur centers (ISC) was not observed after 5 min treatment with nitric oxide (NO) (10(-7) M NO/10(7) cells). The content of these centers was measured by electron spin resonance (ESR) spectroscopy at 16-60 K. However, the appearance of a characteristic ESR signal at g(av) = 2.03 indicated the formation of dinitrosyl iron complex (DNIC) in these cells. These findings suggest that loosely bound non-heme iron (free iron) but not iron from ISC is mainly involved in DNIC formation. ISC might release iron for DNIC formation after their destruction induced by the products of NO oxidation (NO2, N2O3, etc).
Vanin
A F
AF
Institute of Chemical Physics, Russian Academy of Sciences, Moscow.
Men'shikov
G B
GB
Moroz
I A
IA
Mordvintcev
P I
PI
Serezhenkov
V A
VA
Burbaev
D Sh
DSh
D000818:Animals; D002460:Cell Line; D004227:Dithionite / Q000494:pharmacology; D004578:Electron Spin Resonance Spectroscopy; D005615:Freezing; D006358:Hot Temperature; D007501:Iron / Q000378:metabolism*; D007506:Iron-Sulfur Proteins / Q000378:metabolism; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D009569:Nitric Oxide / Q000378:metabolism*; D009589:Nitrogen Oxides / Q000378:metabolism
D007506:Iron-Sulfur Proteins; D009589:Nitrogen Oxides; D004227:Dithionite; D009569:Nitric Oxide; C073661:dinitrosyl iron complex; D007501:Iron