Deletion and insertion mutants of HBsAg particles.
We have found previously that hybrid 22-nm HBsAg particles can be created by insertion of short antigenic sequences into the HBV major envelope protein. We have now performed a detailed deletion mutagenesis of the S gene of HBV encoding HBsAg. Deletion of the 51 C-terminal amino acids including most of the third and all of the fourth hydrophobic domain of the S protein did not affect particle assembly and secretion. However, secretion of 22-nm particles was abolished by minor deletions in the N-terminal region. Insertion and deletion/substitution mutants carrying a poliovirus epitope at the N-terminus and the preS1 region at the C-terminus have been characterized.
Machein
U
U
Institute for Medical Microbiology, University of Mainz, Federal Republic of Germany.
Nagel
R
R
Prange
R
R
Clemen
A
A
Streeck
R E
RE
D004252:DNA Mutational Analysis; D006514:Hepatitis B Surface Antigens / Q000235:genetics / Q000378:metabolism*; D006515:Hepatitis B virus / Q000235:genetics* / Q000254:growth & development; D016296:Mutagenesis; D016254:Mutagenesis, Insertional; D017955:Poliovirus / Q000235:genetics; D011498:Protein Precursors / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D013539:Simian virus 40 / Q000235:genetics; D013329:Structure-Activity Relationship; D014759:Viral Envelope Proteins / Q000235:genetics
D006514:Hepatitis B Surface Antigens; D011498:Protein Precursors; D011994:Recombinant Proteins; D014759:Viral Envelope Proteins; C050806:presurface protein 1, hepatitis B surface antigen