title: Infectivity of bovine leukaemia virus infected cattle: an ELISA for detecting antigens expressed in in vitro cultured lymphocytes.
abstract: A simple ELISA is described for quantifying expression of bovine leukaemia virus (BLV) antigens in short-term cultures of peripheral blood lymphocytes (PBL) isolated from infected cattle. The PBL-ELISA demonstrated that antigen expression levels in infected cattle could vary by more than 50-fold. Inoculation of sheep with dilutions of lymphocytes from two BLV-infected cattle, differentiated in the PBL-ELISA by 50 to 100-fold, suggested that antigen expression levels were correlated with infectivity. Haematological data indicated that increased antigen expression in PBL cultures was associated with an increased number of circulating B-lymphocytes, irrespective of whether or not an animal had lymphocytosis. This supported the hypothesis that BLV-infected cattle that are PBL-ELISA positive are more infectious and may present a greater risk of transmitting the disease. The applicability of the PBL-ELISA to a field situation was assessed with 98 BLV-infected cattle from three commercial dairy herds with infection prevalences of 11%, 23% and 47%. Similar percentages (49%, 50% and 52%) of PBL-ELISA positive cattle were identified among those infected cattle available for testing in the three herds. An additional 22 infected cattle from an experimental herd were tested to assess the stability of antigen expression levels over an 8 month period. Fewer (27%) of these cattle were identified as PBL-ELISA positive and antigen expression levels were generally lower than those observed in the commercial herds. Antigen expression levels in the experimental herd remained stable over the period of the study. The potential of the PBL-ELISA to assist in BLV eradication programs by identifying those seropositive cattle with the greatest potential to transmit infection is discussed.
authors.affiliation: CSIRO Division of Tropical Animal Production, Indooroopilly, Qld., Australia.
authors.forename: J A
authors.initials: JA
authors.lastname: Cowley
authors.forename: J B
authors.initials: JB
authors.lastname: Molloy
authors.forename: C K
authors.initials: CK
authors.lastname: Dimmock
authors.forename: P J
authors.initials: PJ
authors.lastname: Walker
authors.forename: A G
authors.initials: AG
authors.lastname: Bruyeres
authors.forename: W H
authors.initials: WH
authors.lastname: Ward
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000032:analysis*; D001402:B-Lymphocytes; D002417:Cattle; D002478:Cells, Cultured; D016583:Enzootic Bovine Leukosis / Q000150:complications / Q000175:diagnosis* / Q000635:transmission; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D015151:Immunoblotting; D001909:Leukemia Virus, Bovine / Q000276:immunology*; D007958:Leukocyte Count / Q000662:veterinary; D008214:Lymphocytes / Q000382:microbiology*; D008218:Lymphocytosis / Q000209:etiology / Q000662:veterinary; D012680:Sensitivity and Specificity; D012756:Sheep
chemical_list: D000956:Antigens, Viral