Binding of sex-hormone-binding globulin (SHBG) to testicular membranes and solubilized receptors.
Sex-hormone-binding globulin (SHBG) binds to a specific protein on the surface of prostate, epididymis, and a human breast cancer cell line (MCF-7), and is internalized by these cells. The present study demonstrated specific binding of SHBG to receptor on membranes prepared from rat testes. The binding was saturable, specific, and time and temperature dependent. Scatchard analysis of these binding studies suggested that SHBG binds to a single class of sites on testicular membranes with a Kd at 37 degrees C of 5 x 10(-8) M and a binding capacity of 30 +/- 0.6 pmol/mg protein. These binding characteristics are similar to the SHBG receptor on human prostate and MCF-7 cells. Solubilization of the receptor resulted in a 5-fold increase in its binding capacity (158 +/- 0.3 pmol/mg protein) and a 10-fold decrease in binding affinity (Kd at 37 degrees C = 6.5 x 10(-7) M). The apparent molecular weight of the testicular SHBG receptor, as estimated by gel filtration, was M(r) = 174,000.


CONCLUSIONS
a specific binding site for SHBG was identified on testicular membranes. This binding site has been tentatively identified as a SHBG receptor based on its physical properties in testicular membrane preparations and following solubilization.
Porto
C S
CS
Department of Pharmacology, Escola Paulista de Medicina, São Paulo, Brazil.
Abreu
L C
LC
Gunsalus
G L
GL
Bardin
C W
CW
D000818:Animals; D002462:Cell Membrane / Q000378:metabolism*; D002793:Cholic Acids; D002850:Chromatography, Gel; D003902:Detergents; D008297:Male; D008970:Molecular Weight; D011485:Protein Binding; D011817:Rabbits; D051381:Rats; D017208:Rats, Wistar; D011956:Receptors, Cell Surface / Q000302:isolation & purification / Q000378:metabolism*; D012738:Sex Hormone-Binding Globulin / Q000378:metabolism*; D012995:Solubility; D013737:Testis / Q000378:metabolism*
D002793:Cholic Acids; D003902:Detergents; D011956:Receptors, Cell Surface; D012738:Sex Hormone-Binding Globulin; C063572:sex hormone-binding globulin receptor; C028213:3-((3-cholamidopropyl)dimethylammonium)-1-propanesulfonate