title: Infection of the HTLV-I-harbouring T-lymphoblastoid line MT-2 by Epstein-Barr virus.
abstract: Epstein-Barr virus (EBV), a ubiquitous human B-lymphotropic virus, is associated with certain lymphoproliferative diseases of T-cell lineage. To understand the mechanism by which EBV infects T cells, we have tested the susceptibility of various human T-cell lines to the virus. We report here that the HTLV-I-harbouring T-lymphoblastoid line MT-2 carries a high level of CD21/EBV receptors on their surface, adsorbs fluorescein isothiocyanate-labeled EBV, and synthesizes virus latent antigens (EBNA-1 and LMP) following EBV infection. Pretreatment of MT-2 cells with anti-CD21 monoclonal antibody OKB7 inhibited the virus binding as well as the synthesis of virus latent antigen. These data suggest that human T-cells can be infected with EBV via functionally active virus receptors.
authors.affiliation: Department of Virology, Hokkaido University School of Medicine, Sapporo, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Koizumi
authors.forename: X K
authors.initials: XK
authors.lastname: Zhang
authors.forename: S
authors.initials: S
authors.lastname: Imai
authors.forename: M
authors.initials: M
authors.lastname: Sugiura
authors.forename: N
authors.initials: N
authors.lastname: Usui
authors.forename: T
authors.initials: T
authors.lastname: Osato
mesh_terms: D000944:Antigens, Differentiation, B-Lymphocyte / Q000378:metabolism; D000956:Antigens, Viral / Q000378:metabolism; D002460:Cell Line; D004854:Herpesvirus 4, Human / Q000254:growth & development* / Q000276:immunology; D015368:Human T-lymphotropic virus 1 / Q000254:growth & development; D006801:Humans; D007158:Immunologic Techniques; D066298:In Vitro Techniques; D011951:Receptors, Complement / Q000378:metabolism; D017464:Receptors, Complement 3d; D013601:T-Lymphocytes / Q000382:microbiology*; D014764:Viral Proteins / Q000378:metabolism
chemical_list: D000944:Antigens, Differentiation, B-Lymphocyte; D000956:Antigens, Viral; D011951:Receptors, Complement; D017464:Receptors, Complement 3d; D014764:Viral Proteins