Detection of the ethyl- and pentyl-radical adducts of alpha-(4-pyridyl-1- oxide)-N-tert-butylnitrone in rat-liver microsomes treated with ADP, NADPH and ferric chloride.
HPLC-EPR analyses of the reaction mixtures of microsomal suspensions incubated with ADP, ferric chloride, NADPH and alpha-(4-pyridyl-1-oxide)-N-tert-butylnitrone (4-POBN) were performed. In the elution pattern of the reaction mixture, three peaks (peaks 1, 2 and 3) were detected. The radical adducts (1 and 3) were identified as being the pentyl- and ethyl-radical adducts of 4-POBN by comparing their retention times with those of the authentic radical adducts.
Iwahashi
H
H
Laboratory of Molecular Biophysics, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709.
Parker
C E
CE
Tomer
K B
KB
Mason
R P
RP
D000244:Adenosine Diphosphate / Q000494:pharmacology*; D000818:Animals; D002712:Chlorides; D002851:Chromatography, High Pressure Liquid; D004578:Electron Spin Resonance Spectroscopy; D005290:Ferric Compounds / Q000494:pharmacology*; D005609:Free Radicals; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects*; D009249:NADP / Q000494:pharmacology*; D009589:Nitrogen Oxides / Q000737:chemistry*; D011725:Pyridines; D051381:Rats; D011919:Rats, Inbred Strains; D013113:Spin Labels
D002712:Chlorides; D005290:Ferric Compounds; D005609:Free Radicals; D009589:Nitrogen Oxides; D011725:Pyridines; D013113:Spin Labels; C043438:alpha-(4-pyridyl-1-oxide)-N-tert-butylnitrone; D009249:NADP; D000244:Adenosine Diphosphate; C024555:ferric chloride