title: The surface envelope protein gene region of equine infectious anemia virus is not an important determinant of tropism in vitro.
abstract: Virulent, wild-type equine infectious anemia virus (EIAV) is restricted in one or more early steps in replication in equine skin fibroblast cells compared with cell culture-adapted virus, which is fully competent for replication in this cell type. We compared the sequences of wild-type EIAV and a full-length infectious proviral clone of the cell culture-adapted EIAV and found that the genomes were relatively well conserved with the exception of the envelope gene region, which showed extensive sequence differences. We therefore constructed several wild-type and cell culture-adapted virus chimeras to examine the role of the envelope gene in replication in different cell types in vitro. Unlike wild-type virus, which is restricted by an early event(s) for replication in equine dermis cells, the wild-type outer envelope gene chimeras are replication competent in this cell type. We conclude that even though there are extensive sequence differences between wild-type and cell culture-adapted viruses in the surface envelope gene region, this domain is not a determinant of the differing in vitro cell tropisms.
authors.affiliation: Department of Microbiology, College of Veterinary Medicine, North Carolina State University, Raleigh 27606.
authors.forename: S T
authors.initials: ST
authors.lastname: Perry
authors.forename: M T
authors.initials: MT
authors.lastname: Flaherty
authors.forename: M J
authors.initials: MJ
authors.lastname: Kelley
authors.forename: D L
authors.initials: DL
authors.lastname: Clabough
authors.forename: S R
authors.initials: SR
authors.lastname: Tronick
authors.forename: L
authors.initials: L
authors.lastname: Coggins
authors.forename: L
authors.initials: L
authors.lastname: Whetter
authors.forename: C R
authors.initials: CR
authors.lastname: Lengel
authors.forename: F
authors.initials: F
authors.lastname: Fuller
mesh_terms: D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D004279:DNA, Viral; D004859:Equine Infectious Anemia / Q000382:microbiology; D006736:Horses; D004860:Infectious Anemia Virus, Equine / Q000235:genetics* / Q000472:pathogenicity / Q000502:physiology; D007700:Kinetics; D008969:Molecular Sequence Data; D010641:Phenotype; D011533:Proviruses / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D014759:Viral Envelope Proteins / Q000235:genetics*; D014774:Virulence / Q000235:genetics; D014779:Virus Replication
chemical_list: D004279:DNA, Viral; D014759:Viral Envelope Proteins