title: Regeneration of autotransplanted splenic tissue at different implantation sites.
abstract: Inbred animals (Lewis rats) were used to investigate the regeneration of autologously implanted splenic tissue at intra-omental and subcutaneous sites. Quantitative immunohistology with monoclonal antibodies against lymphocytes and macrophages was performed to analyse the cell density of red pulp (RP), periarteriolar lymphoid sheath (PALS), marginal zone (MZ) and follicle, 7-180 days after transplantation. Antigenic, allogeneic and mitogenic stimulation and Northern blotting were also performed. Transplant groups differed from spleen only in the reduced size of PALS; however, quantitative analysis demonstrated subtle differences between spleen and transplants. The cell density of B-cells and ED-1+ macrophages was reduced in the RP, Tsupp/cyt-cells were decreased and B-cells increased in PALS, and B-cells and Thelper-cells reduced in the MZ. No differences could be detected between the transplant groups. Flow-cytometric analysis of cell suspensions from spleen and transplants revealed a reduction of T-cells (OX-19+), MHC-I and transferrin-receptor-bearing cells in both transplant groups, and a decrease in the number of Thelper-cells and ED-3+ macrophages in subcutaneous transplants. Both transplant groups were defective regarding the allogeneic and pokeweed mitogen response. Aberration of the lipopolysaccharide response was restricted to subcutaneous transplants, which additionally showed abnormal expression of interferon-gamma, interleukin-5 and interleukin-6 mRNA. Thus, subtle alterations of the newly developed microenvironment and/or lymphocyte-homing may influence the regeneration of splenic tissue; the implantation site may represent an important parameter in functional reorganisation.
authors.affiliation: Institut für Allgemeine Biologie, Biochemie und Biophysik, Salzburg, Austria.
authors.forename: A
authors.initials: A
authors.lastname: Liaunigg
authors.forename: C
authors.initials: C
authors.lastname: Kastberger
authors.forename: W
authors.initials: W
authors.lastname: Leitner
authors.forename: M E
authors.initials: ME
authors.lastname: Kurz
authors.forename: E S
authors.initials: ES
authors.lastname: Bergmann
authors.forename: M
authors.initials: M
authors.lastname: Seifriedsberger
authors.forename: D
authors.initials: D
authors.lastname: Weinlich
authors.forename: W
authors.initials: W
authors.lastname: Pimpl
authors.forename: J
authors.initials: J
authors.lastname: Thalhamer
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D002454:Cell Differentiation; D003208:Concanavalin A; D005260:Female; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D007150:Immunohistochemistry; D007959:Lymphocyte Culture Test, Mixed; D016131:Lymphocyte Subsets / Q000276:immunology; D008264:Macrophages / Q000276:immunology; D008297:Male; D011043:Pokeweed Mitogens; D051381:Rats; D011917:Rats, Inbred Lew; D011990:Receptors, Transferrin / Q000032:analysis; D012038:Regeneration / Q000276:immunology; D013154:Spleen / Q000502:physiology*; D014182:Transplantation, Autologous; D016042:Transplantation, Heterotopic
chemical_list: D000911:Antibodies, Monoclonal; D000949:Histocompatibility Antigens Class II; D011043:Pokeweed Mitogens; D011990:Receptors, Transferrin; D003208:Concanavalin A