GABAA receptor immunoreactivity in the white matter.
Using immunohistochemical methods with polyclonal antibodies directed against a specific sequence of the beta 1-subunit of the GABAA receptor, we found strong immunoreactivity in the white matter of cat brain. The immunopositive products were present primarily on processes of glial cells, especially astrocytes. Immunoreactivity appeared also on the cell bodies of astrocytes and on the cytoplasmic membranes of neurons. The abundant immunostaining in the white matter suggests that (1) GABAA receptors are present on glial cells in vivo, (2) GABAA receptors may be localized on non-synaptic membranes in the white matter and (3) activation of GABAA receptors may have some trophic effects on preservation of the structure and functional properties of the white matter.
Gu
Q
Q
Department of Ophthalmology, University of British Columbia, Vancouver, Canada.
Velazquez
J L
JL
Angelides
K J
KJ
Cynader
M S
MS
D000595:Amino Acid Sequence; D000818:Animals; D001253:Astrocytes / Q000737:chemistry; D001923:Brain Chemistry / Q000502:physiology*; D002415:Cats; D007150:Immunohistochemistry; D008969:Molecular Sequence Data; D009457:Neuroglia / Q000737:chemistry*; D011963:Receptors, GABA-A / Q000032:analysis*
D011963:Receptors, GABA-A