Replication of human herpesvirus 6 in epithelial cells in vitro.
Mink lung epithelial (NBL-7) cells were shown to be permissive for human herpesvirus 6 (HHV-6) by four independent methods of analysis: detection of infectious virus, viral antigens, viral DNA sequences, and herpesvirus particles. Infection was serially passaged, with minimal cytopathology, for several months demonstrating for the first time that a cell of epithelial origin can support HHV-6 replication.
Simmons
A
A
Division of Medical Virology, Institute of Medical and Veterinary Science, Adelaide, Australia.
Demmrich
Y
Y
La Vista
A
A
Smith
K
K
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D002460:Cell Line; D003470:Culture Media; D003588:Cytopathogenic Effect, Viral; D015342:DNA Probes; D004279:DNA, Viral / Q000032:analysis; D004848:Epithelium / Q000382:microbiology; D005455:Fluorescent Antibody Technique; D015654:Herpesvirus 6, Human / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification / Q000502:physiology*; D006801:Humans; D008168:Lung / Q000382:microbiology*; D008854:Microscopy, Electron; D008907:Mink; D009693:Nucleic Acid Hybridization; D014771:Virion / Q000302:isolation & purification; D014779:Virus Replication
D000956:Antigens, Viral; D003470:Culture Media; D015342:DNA Probes; D004279:DNA, Viral