title: Restriction in the conformational flexibility of apoproteins in the presence of organic cosolvents: a consequence of the formation of "native-like conformation".
abstract: The influence of n-propanol on the overall alpha-helical conformation of beta-globin, apocytochrome C, and the functional domain of streptococcal M49 protein (pepM49) and its consequence on the proteolysis of the respective proteins has been investigated. A significant amount of alpha-helical conformation is induced into these proteins at pH 6.0 and 4 degrees C in the presence of relatively low concentrations of n-propanol. The induction of alpha-helical conformation into the proteins increased as a function of the propanol concentration, the maximum induction occurring around 30% n-propanol. In the case of alpha-globin, the fluorescence of its tryptophyl residues also increased as a function of n-propanol concentration, the midpoint of this transition being around 20% n-propanol. Furthermore, concomitant with the induction of helical conformation into these proteins, the proteolysis of their polypeptide chain by V8 protease also gets restricted. The alpha-helical conformation induced into alpha- and beta-globin by n-propanol decreased as the temperature is raised from 4 to 24 degrees C. In contrast, the alpha-helical conformation of both alpha- and beta-chain (i.e., globin with noncovalently bound heme) did not exhibit such a sensitivity to this change in temperature. However, distinct differences exist between the n-propanol induced "alpha-helical conformation" of globins and the "alpha-helical conformation" of alpha- and beta-chains. A cross-correlation of the n-propanol induced increase in the fluorescence of beta-globin with the corresponding increase in the alpha-helical conformation of the polypeptide chain suggested that the fluorescence increase represents a structural change of the protein that is secondary to the induction of the alpha-helical conformation into the protein (i.e., an integration of the helical conformation induced to the segments of the polypeptide chain to influence the microenvironment of the tryptophyl residues). Presumably, the fluorescence increase is a consequence of the packing of the helical segments of globin to generate a "native-like structure." The induction of alpha-helical conformation into these proteins in the presence of n-propanol and the consequent generation of "native-like conformation" is not unique to n-propanol. Trifluoroethanol, another helix-inducing organic solvent, also behaves in the same fashion as n-propanol. However, in contrast to the proteins described above, n-propanol could neither induce an alpha-helical conformation into performic acid oxidized RNAse-A nor restrict its proteolysis by proteases.(ABSTRACT TRUNCATED AT 400 WORDS)
authors.affiliation: Department of Medicine, Albert Einstein College of Medicine, Bronx, New York 10641.
authors.forename: A S
authors.initials: AS
authors.lastname: Acharya
authors.forename: K S
authors.initials: KS
authors.lastname: Iyer
authors.forename: G
authors.initials: G
authors.lastname: Sahni
authors.forename: K M
authors.initials: KM
authors.lastname: Khandke
authors.forename: B N
authors.initials: BN
authors.lastname: Manjula
mesh_terms: D000433:1-Propanol / Q000494:pharmacology; D000942:Antigens, Bacterial; D001059:Apoproteins / Q000737:chemistry*; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins / Q000737:chemistry; D002352:Carrier Proteins; D003574:Cytochrome c Group / Q000737:chemistry; D045304:Cytochromes c; D005914:Globins / Q000737:chemistry / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D011487:Protein Conformation / Q000187:drug effects; D017433:Protein Structure, Secondary / Q000187:drug effects; D012259:Ribonuclease, Pancreatic / Q000737:chemistry / Q000378:metabolism; D012697:Serine Endopeptidases / Q000378:metabolism; D012997:Solvents / Q000494:pharmacology*; D013050:Spectrometry, Fluorescence; D013696:Temperature; D014357:Trypsin / Q000378:metabolism; D014364:Tryptophan / Q000737:chemistry
chemical_list: D000942:Antigens, Bacterial; D001059:Apoproteins; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D002352:Carrier Proteins; D003574:Cytochrome c Group; D012997:Solvents; C014689:streptococcal M protein; D014364:Tryptophan; D005914:Globins; D045304:Cytochromes c; D000433:1-Propanol; D012259:Ribonuclease, Pancreatic; D012697:Serine Endopeptidases; C028344:glutamyl endopeptidase; D014357:Trypsin