title: Inosine-containing primers in human papillomavirus detection by polymerase chain reaction.
abstract: A PCR protocol has been developed using inosine-containing primers for human papilloma virus (HPV) detection. The HPV16 L1 region from CaSki-positive cells was efficiently amplified by a single reaction and directly analyzed by agarose gel electrophoresis. The method was found to be sensitive and reproducible, and it easy to use for HPV typing and screening.
authors.affiliation: Department of Public Health and Cell Biology, 2nd University of Rome, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Novelli
authors.forename: M
authors.initials: M
authors.lastname: Gennarelli
authors.forename: L
authors.initials: L
authors.lastname: De Santis
authors.forename: P
authors.initials: P
authors.lastname: Angeloni
authors.forename: B
authors.initials: B
authors.lastname: Dallapiccola
mesh_terms: D001483:Base Sequence; D006801:Humans; D066298:In Vitro Techniques; D007288:Inosine / Q000737:chemistry*; D027383:Papillomaviridae / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D007288:Inosine