title: Role of different genes in the virulence and pathogenesis of Aujeszky's disease virus.
abstract: In this study the role of different genes located in the unique short region of the genome of Aujeszky's disease virus was examined. Inactivation of the genes encoding the protein kinase (PK), gp63, and gI reduced virulence of the virus for pigs, in contrast to inactivation of the genes encoding the 28 kDa protein, and gX. There was no correlation between virulence and virus multiplication in vitro or in the oropharynx in vivo. The morphogenesis of the PK mutant was altered. The gI mutant replicated to normal titres in the oropharynx and could be recovered from the trigeminal ganglia but not from other parts of the central nervous system, suggesting that gI facilitates the spread of the virus from neuron to neuron. All mutants induced neutralizing antibody and complete or partial protection against a challenge infection. PK and gp63 were required for the induction of complete protection, although these proteins are reportedly not targets for neutralizing antibody or cytotoxic T cells.
authors.affiliation: Central Veterinary Institute, Department of Virology, Lelystad, The Netherlands.
authors.forename: T G
authors.initials: TG
authors.lastname: Kimman
authors.forename: J M
authors.initials: JM
authors.lastname: Pol
authors.forename: N
authors.initials: N
authors.lastname: de Wind
authors.forename: N
authors.initials: N
authors.lastname: Oei-Lie
authors.forename: A J
authors.initials: AJ
authors.lastname: Berns
authors.forename: A L
authors.initials: AL
authors.lastname: Gielkens
mesh_terms: D000818:Animals; D046508:Culture Techniques; D005814:Genes, Viral; D011558:Herpesvirus 1, Suid / Q000235:genetics* / Q000472:pathogenicity / Q000648:ultrastructure; D008854:Microscopy, Electron; D016254:Mutagenesis, Insertional; D009297:Nasal Mucosa / Q000382:microbiology; D011494:Protein Kinases / Q000235:genetics; D011557:Pseudorabies / Q000382:microbiology*; D013047:Specific Pathogen-Free Organisms; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology*; D014759:Viral Envelope Proteins / Q000235:genetics; D014774:Virulence / Q000235:genetics
chemical_list: D014759:Viral Envelope Proteins; C042966:pseudorabies virus glycoproteins; D011494:Protein Kinases