The identification of 18 nuclear genes required for the expression of the yeast mitochondrial gene encoding cytochrome c oxidase subunit 1.
Eighteen nuclear mutants of the yeast Saccharomyces cerevisae, each disturbed in the biosynthesis of the mitochondrially encoded cytochrome c oxidase subunit 1 (cox1) and each representing a distinct complementation group, have been examined to identify the level at which COX1 expression is affected. RNA blotting revealed that most have a defect in the processing of COX1 precursor-mRNA; only a few are defective in COX1 transcription and/or pre-mRNA stability. In most RNA-processing mutants, the absence of the COX1 messenger results from a defect in the splicing of one or more COX1 introns. In turn, this defect can be ascribed to a mutation in a nuclear gene which is either directly involved in splicing or else acts indirectly by impairing COX1 translation.
Pel
H J
HJ
Department of Molecular Cell Biology, University of Amsterdam, The Netherlands.
Tzagoloff
A
A
Grivell
L A
LA
D015152:Blotting, Northern; D015342:DNA Probes; D003576:Electron Transport Complex IV / Q000235:genetics* / Q000378:metabolism; D005091:Exons; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D007438:Introns; D008928:Mitochondria / Q000378:metabolism; D009154:Mutation; D014176:Protein Biosynthesis; D012323:RNA Processing, Post-Transcriptional / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*; D013057:Spectrum Analysis; D014158:Transcription, Genetic
D015342:DNA Probes; D003576:Electron Transport Complex IV