Effect of anionic polymers on fusion of Sendai virus with human erythrocyte ghosts.
The effect of anionic polymers (dextran sulfate, heparin and chondroitin sulfate) on fusion of Sendai virus with erythrocyte ghosts was studied. The effect of pH on the activity of these anionic polymers was also investigated. In order to examine the interaction of such polymers with the Sendai virion and erythrocyte ghost surfaces, the binding of virions to erythrocyte ghosts and the aggregation of virions and/or erythrocyte ghosts were also measured with respect to the same parameters. It was found that the anionic polymers suppressed the fusion of Sendai virus with erythrocyte ghosts. The order of effectiveness of the polymers in suppression was dextran sulfate greater than heparin greater than chondroitin sulfate, for the application of a same quantity (weight/ml) of the polymers. The lower the pH of the suspending medium, the more effective were the polymers in suppressing virion-erythrocyte ghost aggregation and fusion. The suppression of fusion was dependent on the concentration of the polymers applied: the higher the concentration of the polymer applied, the more the suppression was observed. Evidence from binding studies, turbidity measurements and electrophoretic mobility measurements indicates that the anionic polymers interact preferentially with the virion surface.
Ohki
S
S
Department of Biophysical Sciences, State University of New York, Buffalo 14214.
Arnold
K
K
Srinivasakumar
N
N
Flanagan
T D
TD
D000327:Adsorption; D002809:Chondroitin Sulfates / Q000494:pharmacology; D016264:Dextran Sulfate / Q000494:pharmacology; D004586:Electrophoresis; D004903:Erythrocyte Aggregation / Q000187:drug effects; D004910:Erythrocyte Membrane / Q000737:chemistry / Q000187:drug effects / Q000382:microbiology*; D006493:Heparin / Q000494:pharmacology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D009391:Nephelometry and Turbidimetry; D010222:Parainfluenza Virus 1, Human / Q000737:chemistry / Q000187:drug effects*; D011108:Polymers / Q000494:pharmacology*; D013050:Spectrometry, Fluorescence
D011108:Polymers; D006493:Heparin; D002809:Chondroitin Sulfates; D016264:Dextran Sulfate