title: cAMP activates the sodium pump in cultured cells of the elasmobranch rectal gland.
abstract: The inorganic ion content of rectal gland cells cultured from Squalus acanthias was studied by electron probe analysis in order to determine the effect of stimulation by cAMP. Cell sodium was reduced by 30% (P less than 0.01) at 8 min after exposure to dibutyryl cAMP and theophylline and remained low at 25 and 33 min. Chloride content also fell significantly with stimulation. Although cAMP may activate several transport sites, the results are consistent with a direct effect of stimulation to increase the activity of the sodium pump in shark rectal gland.
authors.affiliation: Department of Medicine, Harvard Medical School, Boston MA.
authors.forename: S
authors.initials: S
authors.lastname: Lear
authors.forename: B J
authors.initials: BJ
authors.lastname: Cohen
authors.forename: P
authors.initials: P
authors.lastname: Silva
authors.forename: C
authors.initials: C
authors.lastname: Lechene
authors.forename: F H
authors.initials: FH
authors.lastname: Epstein
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002712:Chlorides / Q000378:metabolism; D000242:Cyclic AMP / Q000494:pharmacology*; D004284:Dogfish; D004577:Electron Probe Microanalysis; D010758:Phosphorus / Q000378:metabolism; D011188:Potassium / Q000378:metabolism; D012491:Salt Gland / Q000187:drug effects* / Q000378:metabolism; D012964:Sodium / Q000378:metabolism; D000254:Sodium-Potassium-Exchanging ATPase / Q000187:drug effects* / Q000502:physiology
chemical_list: D002712:Chlorides; D010758:Phosphorus; D012964:Sodium; D000242:Cyclic AMP; D000254:Sodium-Potassium-Exchanging ATPase; D011188:Potassium