Immediate autoproteolysis and new proteinases in Entamoeba invadens and Entamoeba moshkovskii trophozoites.
We have examined the sodium dodecyl sulfate (SDS)-induced autoproteolysis of E. invadens (PZ and IP101) and E. moshkovskii (FIC and Laredo) trophozoite lysates. Heat-treated lysates containing parahydroxy-mercuribenzoate (pHMB) of all four strains had undegraded protein patterns. Unheated pHMB-lacking lysates of PZ had two (99 and 90 kDa) and IP101 lysates had three (45, 99, 90 kDa) major proteins, whereas FIC and Laredo lysates had only one (90 kDa). Heat-treatment changed the remaining proteins to smaller ones: 37 in PZ and IP101, 33 and 37 kDa in FIC and Laredo. Unheated lysates run on gelatin-containing "substrate" gels had gelatinases whose sizes were higher in lysates containing pHMB and allowed us to detect a 200 kDa gelatinase in E. invadens strains. Our results indicate that SDS induces immediate autoproteolysis by CPs in non-histolytica trophozoites, whose proteinases appear to be processed by self-digestion, and Entamoeba proteinases vary considerably under the various conditions used to obtain and characterize them.
López-Revilla
R
R
Department of Cell Biology, Centro de Investigación y de Estudios Avanzados del IPN, México, DF.
Báez-Camargo
M
M
D000818:Animals; D001329:Autolysis; D003546:Cysteine Endopeptidases / Q000378:metabolism; D010450:Endopeptidases / Q000378:metabolism*; D004747:Entamoeba / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development; D015800:Protozoan Proteins / Q000378:metabolism*; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D013045:Species Specificity
D015800:Protozoan Proteins; D012967:Sodium Dodecyl Sulfate; D010450:Endopeptidases; D003546:Cysteine Endopeptidases