title: Localization of cGMP after infusion of ANP or nitroprusside in the maturing rat.
abstract: To determine the ontogeny of intrarenal distribution of guanosine 3',5'-cyclic monophosphate (cGMP) formation in response to atrial natriuretic peptide (ANP) or sodium nitroprusside (SNP), adult and neonatal Sprague-Dawley rats were anesthetized and infused for 60 s with rat ANP (5-2,500 micrograms/kg) or SNP (0.1-10.0 mg/kg). cGMP was identified by the immunoperoxidase technique using a specific antibody. In adult rats, infusion of ANP localized cGMP primarily to the glomerular podocytes, whereas stimulation by SNP increased cGMP in the mesangium only (P less than 0.01). In neonatal rats, although overall renal cGMP immunostaining was greater than in adults, specific localization to podocytes (ANP) or mesangium (SNP) resulted only with higher doses of agonists. Although basal generation of cGMP by isolated glomeruli was greater in neonatal rats, the threshold for stimulation by ANP was lower in glomeruli from adult rats. We conclude that in vivo ANP stimulates glomerular particulate guanylate cyclase primarily in the podocytes, whereas SNP stimulates soluble guanylate cyclase localized to the mesangial cells. There is a maturational increase in the sensitivity for activation of glomerular particulate and soluble guanylate cyclase.
authors.affiliation: Department of Pediatrics, University of Virginia School of Medicine, Charlottesville 22908.
authors.forename: R L
authors.initials: RL
authors.lastname: Chevalier
authors.forename: R J
authors.initials: RJ
authors.lastname: Fern
authors.forename: M
authors.initials: M
authors.lastname: Garmey
authors.forename: S S
authors.initials: SS
authors.lastname: el-Dahr
authors.forename: R A
authors.initials: RA
authors.lastname: Gomez
authors.forename: J
authors.initials: J
authors.lastname: De Vente
mesh_terms: D000375:Aging; D000704:Analysis of Variance; D000818:Animals; D000831:Animals, Newborn; D009320:Atrial Natriuretic Factor / Q000008:administration & dosage / Q000494:pharmacology*; D000242:Cyclic AMP / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D005920:Glomerular Mesangium / Q000187:drug effects / Q000378:metabolism; D007150:Immunohistochemistry; D007262:Infusions, Intravenous; D007668:Kidney / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D007672:Kidney Cortex / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D007678:Kidney Glomerulus / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D007684:Kidney Tubules / Q000187:drug effects / Q000378:metabolism; D008297:Male; D009599:Nitroprusside / Q000008:administration & dosage / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D009599:Nitroprusside; D009320:Atrial Natriuretic Factor; D000242:Cyclic AMP