Expression of a cytomegalovirus IE-1-factor VIII cDNA hybrid gene in transgenic mice.
A construct containing the 5' end of the human cytomegalovirus major immediate early gene fused to the human coagulation factor VIII cDNA was used to produce transgenic mice. Two out of five transgenic lines transcribed the construct. The expression was consistently seen in a limited number of tissues and was highest in muscle tissues. This is in contrast to the almost ubiquitous activity demonstrated in earlier studies with the IE-1 enhancer/promoter. Human factor VIII protein was detected immunochemically in muscle tissues at levels several times higher than in human plasma.
Mikkelsen
T R
TR
Department of Molecular Biology and Plant Physiology, Aarhus University, Denmark.
Chapman
B
B
Din
N
N
Ingerslev
J
J
Kristensen
P
P
Poulsen
K
K
Hjorth
J P
JP
D000818:Animals; D015152:Blotting, Northern; D015139:Blotting, Southern; D003001:Cloning, Molecular; D003587:Cytomegalovirus / Q000235:genetics*; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D004742:Enhancer Elements, Genetic; D005167:Factor VII / Q000096:biosynthesis / Q000235:genetics*; D005260:Female; D015870:Gene Expression; D005814:Genes, Viral; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D008822:Mice, Transgenic; D009132:Muscles / Q000166:cytology / Q000502:physiology; D009928:Organ Specificity; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis*; D015183:Restriction Mapping; D014158:Transcription, Genetic
D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; D005167:Factor VII; D004247:DNA