Cyclooctatin, a new inhibitor of lysophospholipase, produced by Streptomyces melanosporofaciens MI614-43F2. Taxonomy, production, isolation, physico-chemical properties and biological activities.
Cyclooctatin has been isolated from Streptomyces melanosporofaciens MI614-43F2 as part of a program designed to find microorganism-produced inhibitors of lysophospholipase. It was purified by chromatography on silica gel, Capcell Pak C18 (HPLC) and Sephadex LH-20 followed by solvent extraction and then isolated as a colorless powder. Cyclooctatin has the molecular formula of C20H34O3. It is competitive with the substrate, and the inhibition constant (Ki) was 4.8 x 10(-6) M.
Aoyagi
T
T
Institute of Microbial Chemistry, Tokyo, Japan.
Aoyama
T
T
Kojima
F
F
Hattori
S
S
Honma
Y
Y
Hamada
M
M
Takeuchi
T
T
D000818:Animals; D001643:Bridged Bicyclo Compounds / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002417:Cattle; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D004224:Diterpenes; D007700:Kinetics; D008099:Liver / Q000187:drug effects*; D008245:Lysophospholipase / Q000037:antagonists & inhibitors*; D008828:Microbiological Techniques; D058428:Silica Gel; D012822:Silicon Dioxide; D013302:Streptomyces / Q000737:chemistry / Q000145:classification / Q000302:isolation & purification
D001643:Bridged Bicyclo Compounds; D004224:Diterpenes; C078947:cyclooctatin; D058428:Silica Gel; D012822:Silicon Dioxide; D008245:Lysophospholipase