Sugar modified oligonucleotides: synthesis, nuclease resistance and base pairing of oligodeoxynucleotides containing 1-(4'-thio-beta-D-ribofuranosyl)-thymine.
XdT12 and dT5XdT6, where X is 1-(4'-thio-beta-d-ribofuranosyl)-thymine, were synthesized. The first oligonucleotide presents a better stability against calf spleen phosphodiesterase than natural dT12. The second one hybridizes with complementary sequence. However the X:A base pairing stability is lower than natural T:A one.
Bellon
L
L
Université de Montpellier II Sciences, URA 488 du CNRS, France.
Morvan
F
F
Barascut
J L
JL
Imbach
J L
JL
D000818:Animals; D001482:Base Composition; D002417:Cattle; D004355:Drug Stability; D007202:Indicators and Reagents; D009682:Magnetic Resonance Spectroscopy; D009691:Nucleic Acid Denaturation; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000378:metabolism; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism*; D013154:Spleen / Q000201:enzymology; D013379:Substrate Specificity; D013872:Thionucleosides; D013936:Thymidine / Q000031:analogs & derivatives*
D007202:Indicators and Reagents; D009841:Oligonucleotides; D013872:Thionucleosides; C074301:1-(4'-thio-beta-ribofuranosyl)thymine; D010727:Phosphoric Diester Hydrolases; D013936:Thymidine