title: [The role of calcium ions in the molecular mechanism of the protective mechanism of exogenous phosphocreatine].
abstract: The role of Ca2+ in the manifestation of the cardioprotective effect of phosphocreatine (PCr) on the ischemic myocardium was studied in isolated rat hearts perfused by the Langendorf method. Under ischemic cardiac arrest induced by a Ca(2+)-free perfusing solution PCr had no protective effect on the ischemic myocardium. PCr accelerated the postischemic restoration of contractility of hearts perfused with a solution containing 0.5 and 1.2 mM Ca2+. The structural analog of PCr, phosphoarginine, possessing a Ca(2+)-binding capacity similar to that of PCr, had no protective effect. The effects of PCr and Ca2+ on the package of sarcolemmal vesiculate lipids were studied by ESR spectroscopy. PCr induced a more dense package of membrane phospholipids at weakly acidic and neutral values of pH (but not at pH 8.5). Although at pH 5.5 Ca2+ did not affect the membrane structure, it potentiated the effect of PCr on sarcolemmal phospholipids. Thus, the protective effect of PCr on the ischemic myocardium is not linked with its ability to bind Ca2+; however, Ca2+ is an indispensable component of the mechanism underlying the protective effect of PCr on the ischemic myocardium.
authors.forename: I V
authors.initials: IV
authors.lastname: Dzhaliashvili
authors.forename: E A
authors.initials: EA
authors.lastname: Konorev
authors.forename: N V
authors.initials: NV
authors.lastname: Medvedeva
authors.forename: V L
authors.initials: VL
authors.lastname: Lakomkin
authors.forename: V A
authors.initials: VA
authors.lastname: Saks
mesh_terms: D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D002413:Cations, Divalent; D003327:Coronary Disease / Q000517:prevention & control; D004578:Electron Spin Resonance Spectroscopy; D006321:Heart / Q000187:drug effects; D066298:In Vitro Techniques; D008297:Male; D009206:Myocardium / Q000378:metabolism; D009943:Organophosphorus Compounds / Q000494:pharmacology; D010725:Phosphocreatine / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D013113:Spin Labels
chemical_list: D002413:Cations, Divalent; D009943:Organophosphorus Compounds; D013113:Spin Labels; D010725:Phosphocreatine; C015441:phospho-L-arginine; D001120:Arginine; D002118:Calcium
vernacular_title: Rol' ionov kal'tsiia v molekuliarnom mekhanizme zashchitnogo deĭstviia ékzogennogo fosfokreatina.