[Effects of paradoxical sleep deprivation on the activity of opiate receptors isolated from synaptic membranes of the rat brain].
The specific binding of 3H-naloxone with opiate receptors isolated from brain synaptic membranes of control and paradoxical sleep deprived rats were studied. This extreme state was shown to reduce the naloxone-binding activity of synaptic membranes by 35% and isolated receptors by 25-28%. The values of Kd and Bmax were calculated for isolated opiate receptors at different stages of purification. Considerable decrease of 3H-naloxone binding sites density (2 times) in the isolated opiate receptors with simultaneous increase of their affinity (3-4,5 times) was found following 24 hours paradoxical sleep deprivation. These findings suggest development of fixed alterations in the structure and functions of integral receptor proteins under extreme influences.
Taranova
N P
NP
Izykenova
G A
GA
D000818:Animals; D001923:Brain Chemistry; D008297:Male; D009270:Naloxone / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011957:Receptors, Opioid / Q000302:isolation & purification / Q000378:metabolism / Q000502:physiology*; D012892:Sleep Deprivation; D012895:Sleep, REM / Q000502:physiology; D013570:Synaptic Membranes / Q000737:chemistry*; D014316:Tritium
D011957:Receptors, Opioid; D014316:Tritium; D009270:Naloxone
Vliianie lisheniia paradoksal'noĭ fazy sna na aktivnost' opiatnykh retseptorov, izolirovannykh iz sinapticheskikh membran mozga krys.