title: Recognition of SV40-VP2 in the infected cell by antipeptide antibodies.
abstract: Antipeptide antibodies were elicited against two synthetic peptides corresponding to amino acids 47-55 and 98-103 of the structural protein VP 2 of SV 40. The induced antibodies proved to be VP 2-specific in an immunoblot. In immunofluorescence these antibodies showed a discrete nuclear and perinuclear staining pattern. In immune electron microscopy studies the induced antibodies did not bind to major virions suggesting that VP 2 is not present at the surface of SV 40 particles.
authors.affiliation: Department of Medical Microbiology and Virology, Ruhr-University Bochum, Federal Republic of Germany.
authors.forename: H J
authors.initials: HJ
authors.lastname: Streckert
authors.forename: H J
authors.initials: HJ
authors.lastname: Sommerfeld
authors.forename: K
authors.initials: K
authors.lastname: Morgenroth
authors.forename: H
authors.initials: H
authors.lastname: Werchau
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology*; D000918:Antibody Specificity; D002213:Capsid / Q000138:chemical synthesis / Q000276:immunology*; D036022:Capsid Proteins; D002460:Cell Line; D005455:Fluorescent Antibody Technique; D016253:Microscopy, Immunoelectron; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology; D011817:Rabbits; D013539:Simian virus 40 / Q000276:immunology* / Q000648:ultrastructure
chemical_list: D000914:Antibodies, Viral; D036022:Capsid Proteins; D010455:Peptides; C442543:VP2 protein, Polyomavirus