[The pharmacological characteristics, immunochemical identification and study of the location of quisqualate receptors in the rat and human brains].
The kinetics of [3H]-L-glutamate binding to brain synaptic membranes (SM) and to glutamate-binding proteins (GBP) was determined with agonist and monoclonal antibodies (MAbs). It was revealed, that rat and human brain GBP have individual protein components with M(r) from 14 to 92 kDa. Quisqualate inhibited [3H]-L-glutamate binding to solubilized and to purified 68 kDa protein component. MAbs have the most activity, and NMDA was failure. It has been shown that 68 kDa component antigen determinants are similar to those of bovine, frog and rat brain synaptic membranes. Anti-GBP monoclonal antibodies blocked functional non-NMDA receptors in isolated frog spinal cord. Immunocytochemistry was done on rat and human brain sections. Distribution of quisqualate receptors was determined with light and electron microscopy. Some properties of vertebrate CNS non-NMDA receptors are discussed.
Dambinova
S A
SA
Bobryshev
Iu V
IuV
Gorodinskiĭ
A I
AI
Margulis
M N
MN
Nikitin
A O
AO
Orlova
E A
EA
Pavlov
I Iu
IIu
Savinov
A Iu
AIu
D000328:Adult; D000818:Animals; D000911:Antibodies, Monoclonal; D001921:Brain / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D005971:Glutamates / Q000276:immunology / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D008024:Ligands; D008565:Membrane Proteins / Q000276:immunology / Q000378:metabolism; D016253:Microscopy, Immunoelectron; D011485:Protein Binding / Q000187:drug effects; D016318:Quisqualic Acid / Q000378:metabolism*; D011895:Rana ridibunda; D051381:Rats; D018091:Receptors, AMPA; D017470:Receptors, Glutamate / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure
D000911:Antibodies, Monoclonal; D005971:Glutamates; D008024:Ligands; D008565:Membrane Proteins; D018091:Receptors, AMPA; D017470:Receptors, Glutamate; D016318:Quisqualic Acid
Farmakologicheskaia kharakteristika, immunokhimicheskaia identifikatsiia i issledovanie lokalizatsii kviskvalatnykh retseptorov golovnogo mozga krysy i cheloveka.