title: Short sequence in L1 region of human papillomaviruses correlates with clinical pictures and grouping by cross-hybridization.
abstract: A consensus primer-mediated polymerase chain reaction devised to amplify a short sequence in L1 region (L1-PCR) efficiently detected genital human papillomaviruses (HPVs) in clinical materials. Nucleotide sequencing of the amplified fragment showed that L1-PCR is also applicable to hitherto unsequenced HPVs. By comparing the amplified 210 nucleotides HPVs were classified into six groups, which are consistent with clinical pictures and a grouping based on cross-hybridization under the stringent condition.
authors.affiliation: Department of Bacteriology, Faculty of Medicine, University of Tokyo.
authors.forename: A
authors.initials: A
authors.lastname: Iwamoto
authors.forename: H
authors.initials: H
authors.lastname: Yoshikawa
authors.forename: K
authors.initials: K
authors.lastname: Kitagawa
authors.forename: H
authors.initials: H
authors.lastname: Igarashi
authors.forename: T
authors.initials: T
authors.lastname: Kawana
authors.forename: H
authors.initials: H
authors.lastname: Yoshikura
mesh_terms: D001483:Base Sequence; D002584:Cervix Uteri / Q000382:microbiology*; D005260:Female; D006801:Humans; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D027383:Papillomaviridae / Q000235:genetics* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D002578:Uterine Cervical Dysplasia / Q000382:microbiology*; D002583:Uterine Cervical Neoplasms / Q000382:microbiology*
chemical_list: D009838:Oligodeoxyribonucleotides