title: Melanotropin as a potential regulator of pigment pattern formation in embryonic skin.
abstract: Frozen tissue sections of developing axolotl embryos were labeled by indirect immunofluorescence with anti-alpha-MSH. Anti-MSH immunoreactivity is first detectable in embryos when neural crest cells are migrating from the neural tube. Antibody labeling is visible around the lateral and ventral edges of the neural tube and in the embryonic ectoderm. As development progresses, the amount of labeling increases greatly, particularly in developing ectoderm. Western blots of soluble proteins extracted from various developmental stages of axolotl embryo ectoderm reveal that MSH activity is associated directly with several high molecular weight components that may be part of the embryonic extracellular matrix. Thus, we suggest that melanotropin activity is present in embryonic axolotl skin, is associated with the extracellular matrix, and is thereby in a position to play a supportive and/or directive role in the establishment of embryonic pigment patterns.
authors.affiliation: Department of Physiology & Cell Biology, University of Kansas, Lawrence 66045.
authors.forename: S
authors.initials: S
authors.lastname: Frost-Mason
authors.forename: D
authors.initials: D
authors.lastname: Walpita
authors.forename: L
authors.initials: L
authors.lastname: McKay
mesh_terms: D000557:Ambystoma / Q000196:embryology; D000818:Animals; D002465:Cell Movement; D004475:Ectoderm / Q000737:chemistry; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D007814:Larva; D009074:Melanocyte-Stimulating Hormones / Q000032:analysis / Q000502:physiology*; D009024:Morphogenesis; D009432:Neural Crest / Q000737:chemistry / Q000166:cytology; D012867:Skin / Q000196:embryology*; D012880:Skin Pigmentation / Q000502:physiology*; D000521:alpha-MSH / Q000276:immunology
chemical_list: D016326:Extracellular Matrix Proteins; D000521:alpha-MSH; D009074:Melanocyte-Stimulating Hormones