title: Reactivation of rat cytomegalovirus in lung allografts: an experimental and immunohistochemical study in rats.
abstract: Reactivation of latent rat cytomegalovirus (RCMV) from a lung allograft or from a recipient was studied in RCMV-mismatched combinations (donor [D]-/recipient [R]+, D+/R-, and D+/R+) with latently infected lung grafts and chronically infected rats in an inbred rat model. Nineteen transplants in a major histocompatibility complex different strain combination (Brown-Norway/Lewis) were immunosuppressed daily (cyclosporine, azathioprine, and prednisolone) from day 3 after orthotopic left lung transplantation and killed on days 3, 6, and 21. Control groups consisted of nine chronically RCMV-infected rats with immunosuppression without transplantation and six allografts with immunosuppression without RCMV infection. Reactivation of latent RCMV was tested by immunohistochemical staining with monoclonal antibodies against RCMV-induced antigens and by plaque assays of the virus in the salivary glands. The following results were obtained: (1) All allotransplants developed acute ongoing rejection on days 3 and 6, and the rejection was resolved on day 21 by immunosuppression. (2) Reactivation was observed in allotransplanted groups, but not in the control rats. (3) In the D+/R+ and D-/R+ groups on days 3 and 6, the number of RCMV-related antigen-positive cells increased in the recipient spleen and lymph nodes and in the bronchus-associated lymphoid tissue of the donor lung in the D-/R+ group, but not in the chronically RCMV-infected controls. (4) In the D+/R- group on day 6, RCMV-induced antigen-positive cells were observed in the spleen and lymph nodes of the recipient and also around the vessels in the recipient lung. (5) In the D-/R+ group, vascular endothelial cells or mildly infiltrated mononuclear cell subpopulations around the vessels in the lung allograft showed weakly positive staining against RCMV-related antigens on day 6. (6) After the initial acute rejection on days 3 and 6 was treated by immunosuppressive drugs, reactivated acute RCMV infection became chronic or latent again on day 21. We conclude that RCMV infection could be transferred with latently infected lung allografts by reactivation of latent RCMV. In rats, as in man, alloimmune responses seemed to have a definite influence on the reactivation of latent RCMV after lung transplantation.
authors.affiliation: Department of Thoracic Surgery, University of Tokyo, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Yagyu
authors.forename: G
authors.initials: G
authors.lastname: Steinhoff
authors.forename: A M
authors.initials: AM
authors.lastname: Duijvestijn
authors.forename: C A
authors.initials: CA
authors.lastname: Bruggeman
authors.forename: H
authors.initials: H
authors.lastname: Matsumoto
authors.forename: P J
authors.initials: PJ
authors.lastname: van Breda Vriesman
mesh_terms: D000818:Animals; D003587:Cytomegalovirus / Q000254:growth & development* / Q000276:immunology; D003586:Cytomegalovirus Infections / Q000276:immunology* / Q000635:transmission; D006084:Graft Rejection / Q000276:immunology* / Q000382:microbiology; D007124:Immunoenzyme Techniques; D007166:Immunosuppressive Agents / Q000627:therapeutic use*; D008168:Lung / Q000276:immunology / Q000382:microbiology; D016040:Lung Transplantation / Q000276:immunology*; D008297:Male; D051381:Rats; D011914:Rats, Inbred BN; D011917:Rats, Inbred Lew; D014184:Transplantation, Homologous; D014775:Virus Activation / Q000276:immunology*
chemical_list: D007166:Immunosuppressive Agents