Retrovirus-mediated gene transfer targeted to malignant glioma cells in murine brain.
A murine model for meningeal metastasis of malignant glioma was developed to study selective gene transfer into tumor cells and to establish a reliable means of determining the rate of tumor cell infection. A murine ecotropic retroviral vector was created in which the Escherichia coli beta-galactosidase gene served as a marker for gene expression from the integrated retrovirus. This retrovirus exhibited a high rate of infectivity in RSV-M mouse glioma cells in vitro. The recombinant retrovirus was injected directly into the cisterna magna of the mice. Staining of beta-galactosidase showed that the rate of gene integration was high in the disseminated glioma cells. These results suggest the possibility of retrovirus-mediated gene therapy for meningeal dissemination of malignant glioma.
Yamada
M
M
Department of Neurosurgery, Center for Adult Diseases, Osaka.
Shimizu
K
K
Miyao
Y
Y
Hayakawa
T
T
Ikenaka
K
K
Nakahira
K
K
Nakajima
K
K
Kagawa
T
T
Mikoshiba
K
K
D000818:Animals; D001483:Base Sequence; D001932:Brain Neoplasms / Q000235:genetics*; D002946:Cisterna Magna; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005798:Genes, Bacterial / Q000235:genetics*; D005822:Genetic Vectors / Q000235:genetics*; D005910:Glioma / Q000235:genetics*; D008578:Meninges; D051379:Mice; D008969:Molecular Sequence Data; D009362:Neoplasm Metastasis; D013539:Simian virus 40 / Q000235:genetics*; D014162:Transfection / Q000379:methods*; D014407:Tumor Cells, Cultured; D001616:beta-Galactosidase / Q000032:analysis / Q000235:genetics*
D001616:beta-Galactosidase