Comparison of EBV-infectivity measured by EBNA-induction and immortalization of human B-cells.
Epstein-Barr virus (EBV) infectivity was measured by two different systems. One was EBV-specific antigen (EBNA) induction in EBV-negative BJAB cells, the other was immortalization of human peripheral blood B-lymphocytes upon EBV infection. Because the sensitivity of the EBNA-induction assay, which takes 2-3 days, was similar with that of B-cell immortalization, which takes 6-8 weeks, the former can be used for the rapid and convenient determination of EBV-infectivity.
Cho
M S
MS
Department of Cellular and Molecular Biology Research, Miles, Inc., Berkeley, CA 94701.
Louie
R E
RE
D000956:Antigens, Viral / Q000096:biosynthesis*; D001402:B-Lymphocytes / Q000382:microbiology; D002460:Cell Line; D002467:Cell Nucleus / Q000276:immunology; D002472:Cell Transformation, Viral; D004279:DNA, Viral / Q000032:analysis; D019309:Epstein-Barr Virus Nuclear Antigens; D005069:Evaluation Studies as Topic; D004854:Herpesvirus 4, Human / Q000276:immunology / Q000472:pathogenicity*; D006801:Humans; D014773:Virology / Q000379:methods; D014774:Virulence
D000956:Antigens, Viral; D004279:DNA, Viral; D019309:Epstein-Barr Virus Nuclear Antigens