Characterization of the aryl hydrocarbon receptor in the human C-4II cervical squamous carcinoma cell line.
Treatment of C-4II human cervical squamous carcinoma cells with 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) gave a concentration-dependent increase in ethoxyresorufin O-deethylase (EROD) activity. The EC50 for this response was approximately 1 nM and the maximum induced activity was 27 pmol/min/mg protein. The molecular properties of the cytosolic and nuclear aryl hydrocarbon (Ah) receptor complex were determined by velocity sedimentation analysis, photoaffinity labeling, gel retardation using a consensus dioxin responsive element (DRE), and DNA-Sepharose, DRE-Sepharose and Sephacryl S-300 gel permeation column chromatography. The apparent molecular masses of the cytosolic and nuclear photoaffinity-labeled Ah receptor complexes were 110 kDa and differed from the corresponding values obtained for the Ah receptor from other animal species. In contrast, most of the other molecular properties of the Ah receptor were not significantly different from those previously reported for other species. The relative Ah-responsiveness of the C-4II cells was assessed by determining the ratio of the induced EROD activity/nuclear Ah receptor levels for a submaximal inducing dose of [3H]TCDD. The induced activity/binding ratio for the human C-4II cells was 0.77 and was at least one order of magnitude lower than the corresponding value for the Ah-responsive rat hepatoma H-4-II E cells.
Wang
X
X
Department of Veterinary Physiology and Pharmacology, Texas A&M University, College Station 77843-4466.
Rosengren
R
R
Morrison
V
V
Santostefano
M
M
Safe
S
S
D001483:Base Sequence; D002294:Carcinoma, Squamous Cell / Q000378:metabolism*; D002460:Cell Line / Q000187:drug effects / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism; D019363:Cytochrome P-450 CYP1A1; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000378:metabolism; D000072317:Polychlorinated Dibenzodioxins / Q000494:pharmacology; D018336:Receptors, Aryl Hydrocarbon; D011955:Receptors, Drug / Q000737:chemistry*; D002583:Uterine Cervical Neoplasms / Q000378:metabolism*
D000072317:Polychlorinated Dibenzodioxins; D018336:Receptors, Aryl Hydrocarbon; D011955:Receptors, Drug; D003577:Cytochrome P-450 Enzyme System; D010088:Oxidoreductases; D019363:Cytochrome P-450 CYP1A1