title: L-glutamate transporter derived from mRNAs of primary glial cultures: expression in Xenopus laevis oocytes.
abstract: A high-affinity sodium-dependent L-glutamate transporter was expressed in Xenopus oocytes after microinjection of poly(A)+ RNA from primary astrocyte cultures from rat brain cortex. mRNA-induced L-glutamate transport was saturable by substrate and shows kinetic features similar to those found in intact glial cell preparations. L-Glutamate accumulation was prevented by rising the external K+ concentration or by coincubation with L-, D-aspartate or D-glutamate. After fractionation by sucrose density gradient, the mRNA encoding for the expressed L-glutamate transporter from glial cells was found in fractions containing messages of 2.05-2.9 kilobases (kb) in length.
authors.affiliation: Centro de Biología Molecular, Facultad de Ciencias, Universidad Autónoma de Madrid, Spain.
authors.forename: R
authors.initials: R
authors.lastname: Alcántara
authors.forename: M
authors.initials: M
authors.lastname: Casado
authors.forename: L
authors.initials: L
authors.lastname: Olivares
authors.forename: C
authors.initials: C
authors.lastname: Giménez
authors.forename: C
authors.initials: C
authors.lastname: Aragón
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D027322:Amino Acid Transport System X-AG; D000818:Animals; D002478:Cells, Cultured; D006023:Glycoproteins / Q000096:biosynthesis / Q000378:metabolism*; D007700:Kinetics; D009457:Neuroglia / Q000378:metabolism*; D009865:Oocytes / Q000378:metabolism*; D011061:Poly A / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D017208:Rats, Wistar; D014982:Xenopus laevis
chemical_list: D027322:Amino Acid Transport System X-AG; D006023:Glycoproteins; D012333:RNA, Messenger; D011061:Poly A; D000469:Alkaline Phosphatase