Elevated stromelysin-1 and reduced collagenase-IV expression in invasive rat embryo fibroblasts expressing E1A deletion mutants + T24-H-ras.
We have studied the in vitro invasive properties of 3 cell lines derived from the co-transfection of rat embryo fibroblasts (REF) with EIA genes deficient in exon 2 and T24-ras. All 3 cell lines showed invasive properties at passage 10 after isolation. Invasive cells expressed elevated levels of stromelysin-1 and reduced levels of 68-kDa type-IV collagenase compared with untransfected REF. In 2 cell lines the invasive capacity increased during in vitro propagation. The expression of stromelysin-1 increased during this process, whereas 68-kDa type-IV collagenase was persistently expressed at reduced levels. In the third clone analyzed, the invasive capacity decreased during culture, in parallel with decreased expression of stromelysin-1. The low level of stromelysin-1 expression observed in this cell line did not result from loss of AP-1-transcription-factor activity, and was not reversed by phorbol-ester treatment.
Engel
G
G
Department of Oncology, Karolinska Institute and Hospital, Stockholm, Sweden.
Popowicz
P
P
Marshall
H
H
Norling
G
G
Svensson
C
C
Auer
G
G
Akusjärvi
G
G
Linder
S
S
D017456:Adenovirus Early Proteins; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D004622:Embryo, Mammalian; D005091:Exons; D005347:Fibroblasts / Q000378:metabolism* / Q000473:pathology; D005354:Fibrosarcoma / Q000235:genetics / Q000473:pathology; D015870:Gene Expression; D011905:Genes, ras; D019278:Matrix Metalloproteinase 3; D020780:Matrix Metalloproteinase 9; D008666:Metalloendopeptidases / Q000235:genetics / Q000378:metabolism*; D003012:Microbial Collagenase / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D009154:Mutation; D009362:Neoplasm Metastasis; D009856:Oncogene Proteins, Viral / Q000235:genetics*; D016755:Proto-Oncogene Proteins c-jun / Q000378:metabolism; D051381:Rats; D014162:Transfection
D017456:Adenovirus Early Proteins; D009856:Oncogene Proteins, Viral; D016755:Proto-Oncogene Proteins c-jun; D008666:Metalloendopeptidases; D019278:Matrix Metalloproteinase 3; D003012:Microbial Collagenase; D020780:Matrix Metalloproteinase 9