[Electron microscopic enzyme cytochemistry of Entamoeba histolytica trophozoite].
Electron microscopic enzyme cytochemical reactions of Entamoeba histolytica trophozoite showed that acid phosphatase (ACP) and cytidine monophosphatase (CMPase) were located in the lysosomes. The lysosome containing enzymes were distributed in the endoplasm and beneath the plasmalemma, and the releasing enzymes by lysosomes excreted outside of the plasmalemma and caused the injury to host cells. The cytochemical positive reactions of catalase and glucose-6-phosphatase (G-6-Pase) showed that E. histolytica contains microbodies and endoplasmic reticulum. The reactive products of peroxidase (POase) were seen in the lysosome-like structure. The reactions of cytochrome oxidase (COase) and succinate dehydrogenase (SDH) were both negative, indicating that E. histolytica lacked mitochondria. The reactions of thiamine pyrophosphatase (TPPase) and nicotinamide adenine dinucleotide phosphatase (NADPase) were both negative, indicating that E. histolytica lacked Golgi body. The reactions of Na(+)-K(+)-ATPase were located on plasmalemma.
Chen
J
J
Department of Parasitology Fujian Medical College, Fuzhou.
D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D004748:Entamoeba histolytica / Q000201:enzymology* / Q000648:ultrastructure; D005952:Glucose-6-Phosphatase / Q000378:metabolism; D006651:Histocytochemistry; D008247:Lysosomes / Q000201:enzymology; D008854:Microscopy, Electron; D009708:Nucleotidases / Q000378:metabolism; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism
D009708:Nucleotidases; C025221:cytidine-5'-monophosphatase; D000135:Acid Phosphatase; D005952:Glucose-6-Phosphatase; D000254:Sodium-Potassium-Exchanging ATPase