[Identification of a segment of the gene for the substance B receptor in the human DNA genome using the polymerase chain reaction].
Polymerase chain reaction was applied to human genomic DNA using primers corresponding to the rat substance P receptor cDNA. As a result, a fragment of 94 b.p. was isolated identical to the fragment 771-864 of the above-mentioned cDNA, with the exception of the G796----A substitution (Val----Ile in the amino acid sequence). A comparison of the established sequence with the published structures of tachykinin receptors of NK-1, NK-2 and NK-3 types allows its assignment to the substance P receptor (NK-1 tachykinin receptor) gene detected in the human genome.
Barinov
A A
AA
Kuriatov
A B
AB
Mertsalov
I B
IB
Martsen
L O
LO
Tsetlin
V I
VI
D000595:Amino Acid Sequence; D001483:Base Sequence; D004247:DNA; D015894:Genome, Human; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D018040:Receptors, Neurokinin-1; D017981:Receptors, Neurotransmitter / Q000235:genetics*; D018039:Receptors, Tachykinin; D012689:Sequence Homology, Nucleic Acid; D015320:Tachykinins / Q000378:metabolism
D018040:Receptors, Neurokinin-1; D017981:Receptors, Neurotransmitter; D018039:Receptors, Tachykinin; D015320:Tachykinins; D004247:DNA
Identifikatsiia uchastka gena retseptora veshchestva P v genomnoĭ DNK cheloveka s pomoshch'iu polimeraznoĭ tsepnoĭ reaktsii.