Tryptophan as an endogenous photosensitizer to elicit harmful effects of ultraviolet B.
Owing to stratospheric ozone depletion (SOD) the natural flux of ultraviolet B (UVB) radiation (290-320 nm) is likely to increase on the earth surface. In our efforts to identify endogenous chromophores which may absorb significantly in the UVB range and subsequently induce phototoxic reactions, we have observed that tryptophan (Trp) was quite photoreactive under UVB. It enhanced considerably the oxygen-dependent photooxidation of tyrosine (Tyr) to dopachrome, a precursor of melanin. Our data suggest that UVB-sensitized Trp produces singlet oxygen (1O2) and superoxide radicals (O2-.), and these reactive forms of oxygen may contribute to membrane-, cytoplasm- and DNA-damaging effects. In the event of an increasing SOD level, other UVB chromophores may also exhibit similar phototoxic properties to lead to a definitive imbalance between cell life, injury and death.
Babu
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Phototoxicology Laboratory, Industrial Toxicology Research Centre, Lucknow, India.
Joshi
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PC
D004295:Dihydroxyphenylalanine / Q000737:chemistry*; D004307:Dose-Response Relationship, Radiation; D045563:Indolequinones; D007211:Indoles; D014442:Monophenol Monooxygenase / Q000528:radiation effects*; D010100:Oxygen; D010777:Photochemistry; D011809:Quinones; D011838:Radiation-Sensitizing Agents; D026082:Singlet Oxygen; D013481:Superoxides; D014364:Tryptophan; D014466:Ultraviolet Rays
D045563:Indolequinones; D007211:Indoles; D011809:Quinones; D011838:Radiation-Sensitizing Agents; D013481:Superoxides; D026082:Singlet Oxygen; C001123:dopachrome; D004295:Dihydroxyphenylalanine; D014364:Tryptophan; D014442:Monophenol Monooxygenase; D010100:Oxygen