Immunokinetics of equine herpesvirus 1 in donkey mares: suppression of secondary cell-mediated response.
To study the immunokinetics of equine herpesvirus 1 (EHV1), donkey mares were immunised with a laboratory strain of EHV1, or with recommended doses of Pneumabort-K vaccine (EHV1 Army 183 strain, formalin-inactivated, with an oil adjuvant) and a booster was given after three months. Humoral immune responses were studied by employing a virus neutralisation (VN) test. A leucocyte migration inhibition test (LMIT) was employed for the assay of cellular immune responses. The VN antibody titre reached 1:64 or 1:128 after primary immunisation and showed a marginal increase (1:256) after secondary immunisation with either of the immunogens. After the primary dose of immunogen, there was a gradual increase in host cellular response which persisted for up to three months. However, on secondary immunisation, cell-mediated immune response was short-lived and weak compared to the primary response with both immunogens. This could be one possible explanation for breakdown of anti-EHV1 immunity leading to abortion in immunised mares.
Singh
M
M
Department of Veterinary Microbiology, Haryana Agricultural University, Hisar, India.
Charan
S
S
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis*; D002464:Cell Migration Inhibition; D005260:Female; D004861:Herpesvirus 1, Equid / Q000276:immunology*; D007111:Immunity, Cellular; D007114:Immunization / Q000662:veterinary; D007117:Immunization, Secondary / Q000662:veterinary; D007700:Kinetics; D009500:Neutralization Tests; D010527:Perissodactyla / Q000276:immunology*; D014765:Viral Vaccines / Q000276:immunology*
D000914:Antibodies, Viral; C076173:Pneumabort-K; D014765:Viral Vaccines