Double antibody sandwich ELISA for the detection of rubella virus antigen.
We developed enzyme linked immunosorbent assay (ELISA) for the detection of rubella virus antigen, using two monoclonal antibodies, MC-7 and MC-22. The double antibody sandwich ELISA method was carefully standardized and found to be sensitive enough to detect as small as 2.5 ng protein of rubella virus. The infective titers by the double antibody sandwich ELISA closely related to those judged by interference of vesicular stomatitis virus in RK-13 cells. The method is simple, sensitive, and readily applicable to the detection of rubella virus.
Komatsu
H
H
Department of Virology, Kitasato Institute, Tokyo, Japan.
Suzuki
Y
Y
Matumoto
M
M
D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology*; D000956:Antigens, Viral / Q000032:analysis*; D002460:Cell Line; D006224:Cricetinae; D004797:Enzyme-Linked Immunosorbent Assay; D005347:Fibroblasts / Q000382:microbiology; D007668:Kidney; D008647:Mesocricetus; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology; D012412:Rubella virus / Q000276:immunology*; D014721:Vesicular stomatitis Indiana virus / Q000502:physiology; D014759:Viral Envelope Proteins / Q000276:immunology*; D014762:Viral Interference
D000914:Antibodies, Viral; D000956:Antigens, Viral; D014759:Viral Envelope Proteins; C040901:rubella antibodies; C061826:E1 envelope protein, Rubella virus