An immunoblotting procedure for detection of antibodies against bovine leukemia virus in cattle.
A sensitive protein immunoblotting (Western blot) procedure has been developed for detecting anti-BLV antibodies in cattle sera. The antibodies against most of the major viral proteins could be detected. This procedure does not give any non-specific background staining and there is absence of any erroneous results due to utilisation of purified viral preparations. The procedure has been applied for detection of antibodies to BLV in a set of 74 sera samples and it has been compared with other commonly used serological tests like ELISA and agar gel immunodiffusion test.
Grover
Y P
YP
Institut National de la Santé et de la Recherche Médicale, Unité 117, Bordeaux, France.
Guillemain
B
B
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D015153:Blotting, Western; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay; D005779:Immunodiffusion; D001909:Leukemia Virus, Bovine / Q000276:immunology*; D012680:Sensitivity and Specificity
D000914:Antibodies, Viral