A rapid method for starting a culture for the establishment of Epstein-Barr virus-transformed human lymphoblastoid cell lines.
We developed a simple and efficient procedure for the establishment of Epstein-Barr virus-transformed human lymphoblastoid cell lines. B-lymphocytes were obtained by centrifugation after hemolysis of red cells with a hemolysis buffer, instead of Ficoll-Parque gradient. We can start a primary culture within 15 min by using this method.
Fukushima
Y
Y
Division of Medical Genetics, Saitama Children's Medical Center, Japan.
Ohashi
H
H
Wakui
K
K
Nishida
T
T
Oh-ishi
T
T
D001402:B-Lymphocytes / Q000166:cytology*; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral; D004854:Herpesvirus 4, Human / Q000502:physiology; D006801:Humans; D013997:Time Factors