title: Smokeless tobacco contains an inhibitor of prolyl hydroxylase activity.
abstract: The effects of smokeless tobacco extract (STE) and various constituents of STE on prolyl hydroxylase activity were determined using enzyme extracted from chick embryos. STE inhibited prolyl hydroxylase activity in a concentration-dependent manner, but nicotine and anabasine had essentially no effect. Enzymatic activity was inhibited by zinc, but not by the other inorganic elements in STE; however, the zinc concentration in STE was not high enough to produce the observed inhibition. The inhibition by STE was diminished by increasing concentrations of 2-oxoglutarate, but not by increasing concentrations of other cofactors. Thus, STE contains an inhibitor of prolyl hydroxylase which may be competitive with 2-oxoglutarate.
authors.affiliation: Department of Pharmacology & Toxicology, University of Louisville, KY 40292.
authors.forename: R J
authors.initials: RJ
authors.lastname: Galvin
authors.forename: W K
authors.initials: WK
authors.lastname: Ramp
authors.forename: L G
authors.initials: LG
authors.lastname: Lenz
authors.forename: W M
authors.initials: WM
authors.lastname: Pierce
mesh_terms: D000818:Animals; D002642:Chick Embryo; D002849:Chromatography, Gas; D010947:Plants, Toxic; D011393:Procollagen-Proline Dioxygenase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D014030:Tobacco, Smokeless / Q000032:analysis / Q000494:pharmacology*
chemical_list: D011393:Procollagen-Proline Dioxygenase