title: [Multiple sclerosis: IL-2, IFN-tau and PGE2 secretion in mononuclear cell cultures stimulated with PHA and the effect of these cytokines on oligodendroglial cells].
abstract: The production of interferon-tau (IFN-tau), interleukin-2 (IL-2) and prostaglandin-E2 (PGE2) in peripheral blood mononuclear cells (PBMC) stimulated with phytohemagglutinin (PHA) and the repercussion which these cytokines have on the growth and activity of the 2', 3' and cyclonucleotide-3'-phosphohydrolase (CNP) of cultures of rat oligodendrocytes were analyzed. The study was carried out in a group of patients (20 with active disease, 23 in remission) with defined multiple sclerosis (MS), a group of subjects with other central nervous system diseases (OCNSD) and a group of 20 healthy (HS). Increases in PGE2 were found in PBMC cultures of patients with MS (p < 0.0005, ANOVA test) and the production of PGE2 in patients with active MS correlated positively (R2 = 0.743, p = 0.02) with CNP activity in the oligodendroglial cultures. These results suggest that PGE2 may influence in the re-myelinization process.
authors.affiliation: Servicio de Inmunología, Hospital de Bellvitge-Prínceps d'Espanya, Barcelona.
authors.forename: A
authors.initials: A
authors.lastname: Sierra
authors.forename: I
authors.initials: I
authors.lastname: Ferrer
authors.forename: T
authors.initials: T
authors.lastname: Arbizu
authors.forename: E
authors.initials: E
authors.lastname: Buendía
mesh_terms: D063309:2',3'-Cyclic Nucleotide 3'-Phosphodiesterase; D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases / Q000378:metabolism; D000293:Adolescent; D000328:Adult; D000818:Animals; D002478:Cells, Cultured; D015232:Dinoprostone / Q000378:metabolism*; D005260:Female; D006801:Humans; D007370:Interferon Type I; D007371:Interferon-gamma / Q000378:metabolism*; D007376:Interleukin-2 / Q000378:metabolism*; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000276:immunology / Q000378:metabolism*; D009836:Oligodendroglia / Q000378:metabolism*; D010727:Phosphoric Diester Hydrolases; D010835:Phytohemagglutinins / Q000494:pharmacology; D011257:Pregnancy Proteins; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D007370:Interferon Type I; D007376:Interleukin-2; D010835:Phytohemagglutinins; D011257:Pregnancy Proteins; C020947:interferon tau; D007371:Interferon-gamma; D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases; D010727:Phosphoric Diester Hydrolases; D063309:2',3'-Cyclic Nucleotide 3'-Phosphodiesterase; C571337:CNP protein, human; C571339:Cnp protein, rat; D015232:Dinoprostone
vernacular_title: Esclerosis múltiple: secreción de IL-2, IFN-tau y PGE2 en cultivo de células mononucleares estimuladas con PHA y efecto de estas citocinas sobre las células oligodendrogliales.