Detection of anti-gag antibodies of feline immunodeficiency virus in cat sera by enzyme-linked immunosorbent assay.
Using gag protein of feline immunodeficiency virus (FIV) expressed in Escherichia coli, an enzyme-linked immunosorbent assay (ELISA) system was developed for detection of antibodies to FIV gag protein in cat sera. With serum samples from cats experimentally infected with several strains and an infectious molecular clone of FIV, increases of the antibody titers to FIV gag protein were observed in all cases by the ELISA at early stage of infection. When we examined a total of 415 field cat sera which were previously tested by an indirect immunofluorescence assay (IFA), 9 (12.9%) out of 70 IFA positive sera were judged as negative by the ELISA. However, all 3 serum samples tested among the 9 IFA positive sera had antibodies to gp130 but not to p26 by a radioimmunoprecipitation assay. The results indicated that some IFA positive sera did not have antibodies to the p26 though they have antibodies to other proteins specific for FIV.
Furuya
T
T
Department of Veterinary Microbiology, Faculty of Agriculture, University of Tokyo, Japan.
Hasegawa
A
A
Miyazawa
T
T
Miki
K
K
Mikami
T
T
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D002415:Cats; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D015683:Gene Products, gag / Q000276:immunology*; D016089:Immunodeficiency Virus, Feline / Q000276:immunology*; D008297:Male; D015531:Radioimmunoprecipitation Assay
D000914:Antibodies, Viral; D015683:Gene Products, gag