Interaction of plasma gelsolin with tropomyosin.
Horse plasma gelsolin labelled with benzophenone-4-isothiocyanate can be photochemically cross-linked to rabbit cardiac tropomyosin. The cross-linking proceeds with greater efficiency in calcium-containing buffers. Further evidence for interaction between these proteins is provided by retention of fluorescently labelled gelsolin on tropomyosin-agarose affinity columns and by the ability of tropomyosin to cause an increase in the fluorescence intensity of gelsolin labelled with fluorescein-5-isothiocyanate. Both of these effects require the presence of calcium ions.
Koepf
E K
EK
Department of Chemistry, University of British Columbia, Vancouver, Canada.
Burtnick
L D
LD
D000818:Animals; D002135:Calcium-Binding Proteins / Q000097:blood* / Q000302:isolation & purification; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D005456:Fluorescent Dyes; D018260:Gelsolin; D006736:Horses; D007700:Kinetics; D008840:Microfilament Proteins / Q000097:blood* / Q000302:isolation & purification; D009132:Muscles / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism; D011485:Protein Binding; D011817:Rabbits; D014335:Tropomyosin / Q000302:isolation & purification / Q000378:metabolism*
D002135:Calcium-Binding Proteins; D005456:Fluorescent Dyes; D018260:Gelsolin; D008840:Microfilament Proteins; D014335:Tropomyosin