title: [Antigenicity tests of suplatast tosilate (IPD-1151T)].
abstract: Antigenicity of suplatast tosilate (IPD-1151T) was investigated in guinea pigs and mice. The results were as follows: 1. Homologous passive cutaneous anaphylaxis (PCA), active systemic anaphylaxis (ASA), active cutaneous anaphylaxis (ACA) and Schultz-Dale reaction tests were carried out using guinea pigs which were immunized orally with IPD-1151T alone or subcutaneously with IPD-1151T and Freund's complete adjuvants (CFA). Positive reactions in these tests were not produced by eliciting injection of IPD-1151T or its metabolite, M-1. On the other hand, the sensitization of ovalbumin (OVA) with CFA produced positive reactions in all of PCA, ASA, ACA and Schultz-Dale tests. 2. Heterologous passive cutaneous anaphylaxis (PCA) test using rats was carried out using two strains of mice (C3H/He and BALB/c) which were immunized orally with IPD-1151T alone or intraperitoneally with IPD-1151T and aluminum hydroxide gel (Alum) as an adjuvant. No animals showed positive reaction to both eliciting antigens, IPD-1151T and M-1. On the other hand, a positive reaction in PCA test to eliciting antigen, OVA, was obtained in rats treated with sera of mice sensitized with OVA plus Alum. 3. These findings showed that IPD-1151T had no antigenicity in guinea pigs and mice.
authors.affiliation: Drug Safety Laboratory, Taiho Pharmaceutical Co., Ltd., Tokushima, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Kouchi
authors.forename: A
authors.initials: A
authors.lastname: Ohuchida
authors.forename: Y
authors.initials: Y
authors.lastname: Maeda
authors.forename: S
authors.initials: S
authors.lastname: Katayama
authors.forename: M
authors.initials: M
authors.lastname: Kurokawa
authors.forename: T
authors.initials: T
authors.lastname: Mizumoto
authors.forename: M
authors.initials: M
authors.lastname: Nakagawa
authors.forename: H
authors.initials: H
authors.lastname: Manabe
authors.forename: S
authors.initials: S
authors.lastname: Sugimoto
authors.forename: R
authors.initials: R
authors.lastname: Yoshida
mesh_terms: D000284:Administration, Oral; D000707:Anaphylaxis; D000818:Animals; D000917:Antibody Formation; D001190:Arylsulfonates / Q000008:administration & dosage / Q000276:immunology*; D006168:Guinea Pigs; D006633:Histamine Antagonists / Q000008:administration & dosage / Q000276:immunology*; D007274:Injections, Intraperitoneal; D007279:Injections, Subcutaneous; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D051381:Rats; D011919:Rats, Inbred Strains; D013452:Sulfonium Compounds / Q000008:administration & dosage / Q000276:immunology*
chemical_list: D001190:Arylsulfonates; D006633:Histamine Antagonists; D013452:Sulfonium Compounds; C073028:suplatast tosilate