Properties of the guinea-pig lung platelet-activating factor receptor encoded by the cloned cDNA.
Platelet-activating factor (PAF) is a naturally occurring phospholipid possessing proinflammatory and shock-inducing activities. Although PAF exerts these functions via specific receptors, the molecular structures of the receptor has not been clarified. We have isolated a cDNA coding for PAF receptor through expression cloning. The PAF receptor expressed in Xenopus laevis oocytes and COS-7 cells showed pharmacological properties consistent with those in previous reports. Sequence analysis revealed that PAF receptor belongs to the superfamily of G-protein-coupled receptors.
Honda
Z
Z
Department of Physiological Chemistry and Nutrition, Faculty of Medicine, University of Tokyo, Japan.
Nakamura
M
M
Seyama
Y
Y
Shimizu
T
T
D000818:Animals; D002460:Cell Line; D003001:Cloning, Molecular; D004247:DNA; D019204:GTP-Binding Proteins / Q000378:metabolism; D006168:Guinea Pigs; D008168:Lung / Q000378:metabolism*; D010972:Platelet Activating Factor / Q000378:metabolism*; D010980:Platelet Membrane Glycoproteins; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism*; D043562:Receptors, G-Protein-Coupled; D014982:Xenopus laevis
D010972:Platelet Activating Factor; D010980:Platelet Membrane Glycoproteins; D011956:Receptors, Cell Surface; D043562:Receptors, G-Protein-Coupled; C039351:platelet activating factor receptor; D004247:DNA; D019204:GTP-Binding Proteins