Studies on carbohydrate-binding proteins using liposome-based systems--I. Preparation of neoglycoprotein-conjugated liposomes and the feasibility of their use as drug-targeting devices.
1. Five types of neoglycoprotein-coupled liposomes were prepared in order to investigate their potential utility as new types of drug-targeting devices which exploit cellular functions of carbohydrate-binding proteins. 2. These preparations were shown to be stable at 37 degrees C for 24 hr and at 7 degrees C over 4 months. 3. An inhibition assay in an in vitro system using human adenocarcinoma cells indicated the high affinity binding of neoglycoprotein-conjugated liposomes. The inhibitory potency correlated with both the type and the amount of immobilized neoglycoproteins on liposomes. 4. A tissue distribution assay in an in vivo system using Ehrlich solid tumor-bearing mice showed the feasibility of the application of [125I]neoglycoprotein-conjugated liposomes as drug-targeting devices, based on carbohydrate-protein interactions.
Yamazaki
N
N
Industrial Products Research Institute, Agency of Industrial Science and Technology, Ibaraki, Japan.
Kojima
S
S
Gabius
S
S
Gabius
H J
HJ
D050260:Carbohydrate Metabolism; D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid; D003110:Colonic Neoplasms; D004337:Drug Carriers; D006023:Glycoproteins / Q000008:administration & dosage* / Q000378:metabolism; D006801:Humans; D008081:Liposomes; D008297:Male; D008969:Molecular Sequence Data; D009928:Organ Specificity; D011956:Receptors, Cell Surface / Q000378:metabolism; D014407:Tumor Cells, Cultured
D004337:Drug Carriers; D006023:Glycoproteins; D008081:Liposomes; D011956:Receptors, Cell Surface