Cloning of the mouse hypothalamic preprothyrotropin-releasing hormone (TRH) cDNA and tissue distribution of its mRNA.
A complementary DNA (cDNA) for mouse hypothalamic preprothyrotropin-releasing hormone (TRH) was isolated and characterized using three different combinations of the polymerase chain reaction (PCR). Using this cDNA, we examined the tissue distribution of expression of the mouse preproTRH gene and evaluated the evolutionary basis of the preproTRH gene by comparison of the cloned sequence with sequences of preproTRH in other species. The deduced protein sequence of the mouse preproTRH contained 256 amino acids featured by possessing an insertion of one amino acid as compared with that of rat preproTRH. Five repetitive copies of the TRH progenitor sequence (Lys-Arg-Gln-His-Pro-Gly-Arg/Lys-Arg) were found in the mouse preproTRH. Northern blot analysis showed an apparent single band of hypothalamic mRNA of approximately 1600 base pairs in length. The homology of the coding region of the mouse preproTRH with the rat and human preproTRH is 92 and 65% at the nucleic acid level, respectively, and 88 and 56% at the amino acid level, respectively. By means of the PCR procedure, a characteristic expression of the preproTRH mRNA was observed solely in the mouse hypothalamus and testis. Moreover, it was noteworthy to find that the degree of homology to the region containing the sixth TRH-coding sequence of the human preproTRH was higher in the mouse preproTRH than the rat counterpart.(ABSTRACT TRUNCATED AT 250 WORDS)
Satoh
T
T
First Department of Internal Medicine, Gunma University School of Medicine, Maebashi, Japan.
Yamada
M
M
Monden
T
T
Iizuka
M
M
Mori
M
M
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D005796:Genes; D007031:Hypothalamus / Q000378:metabolism*; D008297:Male; D051379:Mice / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010446:Peptide Fragments / Q000235:genetics; D016133:Polymerase Chain Reaction; D011498:Protein Precursors / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats / Q000235:genetics; D012045:Regulatory Sequences, Nucleic Acid; D012091:Repetitive Sequences, Nucleic Acid; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D013737:Testis / Q000378:metabolism; D013973:Thyrotropin-Releasing Hormone / Q000235:genetics*
D010446:Peptide Fragments; D011498:Protein Precursors; D012333:RNA, Messenger; C063596:prepro-thyrotropin-releasing hormone; D013973:Thyrotropin-Releasing Hormone; D004247:DNA