title: Detection of herpes simplex virus in gingival tissue.
abstract: The presence of herpes simplex virus (HSV) antigens was shown by immunofluorescence staining in 26 of 66 (39.3%) specimens of clinically healthy gingiva, but only one sample contained infectious virus. HSV DNA sequences were clearly identified in intact gingival cells by dot blot hybridization in one specimen, and a weak pattern in a second one. Both specimens harbored viral antigens. These findings of viral genome and protein expression suggest that the virus is present in the latent form in the gingiva.
authors.affiliation: Virology Department, Hebrew University Hadassah Medical School, Jerusalem, Israel.
authors.forename: R
authors.initials: R
authors.lastname: Amit
authors.forename: A
authors.initials: A
authors.lastname: Morag
authors.forename: Z
authors.initials: Z
authors.lastname: Ravid
authors.forename: N
authors.initials: N
authors.lastname: Hochman
authors.forename: J
authors.initials: J
authors.lastname: Ehrlich
authors.forename: Z
authors.initials: Z
authors.lastname: Zakay-Rones
mesh_terms: D000328:Adult; D001345:Autoradiography; D004247:DNA / Q000032:analysis; D015342:DNA Probes; D004279:DNA, Viral / Q000032:analysis; D005455:Fluorescent Antibody Technique; D016679:Genome, Viral; D005881:Gingiva / Q000382:microbiology*; D006801:Humans; D015151:Immunoblotting; D009693:Nucleic Acid Hybridization; D012016:Reference Values; D018139:Simplexvirus / Q000235:genetics / Q000302:isolation & purification*; D014776:Virus Cultivation / Q000379:methods
chemical_list: D015342:DNA Probes; D004279:DNA, Viral; D004247:DNA