Lethal oxidative damage to human immunodeficiency virus by human recombinant myeloperoxidase.
Human recombinant myeloperoxidase was evaluated in a cell-free system for its inactivation properties on the replication of human immunodeficiency virus, HTLV-IIIB. In the presence of a hydrogen peroxide generating system (glucose and glucose oxidase) and sodium thiocyanate, the recombinant enzyme inhibited virus-induced syncytium formation and viral replication without causing any cytopathic effects on SupT1 reporter cells. In addition, U937 monocytoid cells, chronically infected with HIV1, were exposed to recombinant myeloperoxidase (10 U/ml) and monitored during 48 h for the accumulation of intracellular p24 viral antigen. Under these conditions, the recombinant enzyme significantly reduced intracellular viral replication without affecting cell viability.
Moguilevsky
N
N
Applied Genetics, University of Brussels, Nivelles, Belgium.
Steens
M
M
Thiriart
C
C
Prieels
J P
JP
Thiry
L
L
Bollen
A
A
D002460:Cell Line; D005947:Glucose / Q000378:metabolism; D005949:Glucose Oxidase / Q000378:metabolism; D006678:HIV / Q000187:drug effects*; D016655:HIV Core Protein p24 / Q000378:metabolism; D015497:HIV-1 / Q000187:drug effects*; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D010084:Oxidation-Reduction; D009195:Peroxidase / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000494:pharmacology; D013861:Thiocyanates / Q000494:pharmacology; D014779:Virus Replication / Q000187:drug effects
D016655:HIV Core Protein p24; D011994:Recombinant Proteins; D013861:Thiocyanates; C024553:sodium thiocyanate; D006861:Hydrogen Peroxide; D005949:Glucose Oxidase; D009195:Peroxidase; D005947:Glucose