title: Cytochrome composition and oxygen-dependent respiration-driven proton translocation in Wolinella curva, Wolinella recta, Bacteroides ureolyticus, and Bacteroides gracilis.
abstract: The membrane fractions of the microaerobically grown type strains of Wolinella curva, Wolinella recta, Bacteroides ureolyticus, and Bacteroides gracilis contained membrane-bound cytochrome b, cytochrome c, and CO-binding cytochrome c. Soluble cytochrome c and CO-binding cytochrome c were also present. Although B. gracilis is oxidase negative, it possessed cytochrome c. With H2 or formate as the electron donor, proton efflux from anaerobic cells occurred upon addition of a pulse of oxygen. With formate as the electron donor, the H+/O ratios of W. curva, W. recta, B. ureolyticus, and B. gracilis were 0.75, 1.66, 2.06, and 2.04, respectively. With H2 as the electron donor, the H+/O ratios of W. curva, B. ureolyticus, and B. gracilis were 1.25, 1.97, and 2.36, respectively. Proton translocation was inhibited by the protonophore carbonylcyanide m-chlorophenylhydrazone. The results confirm that the organisms are not anaerobes but are microaerophiles capable of respiring with oxygen.
authors.affiliation: Department of Biology, Virginia Polytechnic Institute and State University, Blacksburg 24061.
authors.forename: Y H
authors.initials: YH
authors.lastname: Han
authors.forename: R M
authors.initials: RM
authors.lastname: Smibert
authors.forename: N R
authors.initials: NR
authors.lastname: Krieg
mesh_terms: D001439:Bacteroides / Q000737:chemistry*; D001693:Biological Transport, Active; D002167:Campylobacter / Q000737:chemistry*; D002248:Carbon Monoxide / Q000378:metabolism; D003573:Cytochrome b Group / Q000032:analysis; D003574:Cytochrome c Group / Q000032:analysis; D003580:Cytochromes / Q000737:chemistry*; D005561:Formates / Q000378:metabolism; D006859:Hydrogen / Q000378:metabolism; D008566:Membranes / Q000737:chemistry; D010084:Oxidation-Reduction; D011522:Protons
chemical_list: D003573:Cytochrome b Group; D003574:Cytochrome c Group; D003580:Cytochromes; D005561:Formates; D011522:Protons; D002248:Carbon Monoxide; D006859:Hydrogen