title: Identification of multiple biological factors in rabbit serum that modulate dopamine-mediated aortic constriction.
abstract: A bioassay was established using freshly prepared rabbit aortic strip to monitor the effects of various biological factors contained in rabbit serum that modulate vasoconstriction. Serotonin was shown to be a major vasoconstriction modulator in rabbit serum. A rapid procedure is described for its isolation from rabbit serum by sequential gel filtration chromatography and reversed-phase HPLC using C4, C18, and C2/C18 columns with an overall cumulative yield of about 20%. It was also noted that rabbit serum contains multiple biological factors that modulate dopamine-mediated aortic contraction other than serotonin. A potent vasoconstriction inhibitor was identified in rabbit serum which appears to be a novel regulator that mediates its effect via both alpha-adrenergic and serotonin receptors.
authors.affiliation: Population Council, New York, New York 10021.
authors.forename: M G
authors.initials: MG
authors.lastname: Leone
authors.forename: B
authors.initials: B
authors.lastname: Silvestrini
authors.forename: M
authors.initials: M
authors.lastname: Palmery
authors.forename: C Y
authors.initials: CY
authors.lastname: Cheng
mesh_terms: D000818:Animals; D001013:Aorta, Thoracic / Q000502:physiology*; D001685:Biological Factors / Q000097:blood*; D004298:Dopamine / Q000502:physiology; D066298:In Vitro Techniques; D011817:Rabbits; D011942:Receptors, Adrenergic, alpha / Q000502:physiology; D011985:Receptors, Serotonin / Q000502:physiology; D012701:Serotonin / Q000097:blood / Q000502:physiology; D014661:Vasoconstriction / Q000502:physiology*
chemical_list: D001685:Biological Factors; D011942:Receptors, Adrenergic, alpha; D011985:Receptors, Serotonin; D012701:Serotonin; D004298:Dopamine