title: Effect of bleeding site on clinical laboratory testing of rats: orbital venous plexus versus posterior vena cava.
abstract: We sought to determine if there were any differences in the results of clinical laboratory tests between blood samples collected from the orbital venous plexus and the posterior vena cava of adult male rats. Thirty healthy adult male Sprague Dawley rats were anesthetized by ether inhalation, and blood samples were collected successively from the orbital venous plexus (OVP) and the posterior vena cava (PVC) for hematologic (n = 10), serum chemistry (n = 10), and coagulation (n = 10) analyses. The prothrombin and partial thromboplastin times of samples from the OVP were prolonged (17% and 288%, respectively) when compared with samples from the PVC. Respective hematologic biases were as follows: red blood cell count (7%), hemoglobin (6%), hematocrit (5%), mean corpuscular volume (-3%), mean corpuscular hemoglobin (-1%), mean corpuscular hemoglobin content (1%), white blood cell count (13%), and platelet count (-7%). Respective serum chemistry biases were as follows: sorbitol dehydrogenase (-7%), glucose (-7%), blood urea nitrogen (-10%), creatinine (-2%), total protein (4%), albumin (2%), globulin (9%), alkaline phosphatase (5%), lactate dehydrogenase (-6%), aspartate aminotransferase (-5%), alanine aminotransferase (-2%), total bilirubin (0%), direct bilirubin (0%), magnesium (-17%), sodium (4%), potassium (0), chloride (4%), calcium (-2%), phosphorous (-17%), cholesterol (3%), triglycerides (24%), creatinine kinase (-8%), 5'nucleotidase (0%), and total bile acids (4%). For hematologic testing, there were no biologically significant differences between samples collected from the OVP and PVC. The coagulation times and serum Mg and P showed biologically significant differences between samples collected from the OVP and PVC.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Procter & Gamble Company, Human and Environmental Safety Division, Cincinnati, OH 45239-8707.
authors.forename: G W
authors.initials: GW
authors.lastname: Dameron
authors.forename: K W
authors.initials: KW
authors.lastname: Weingand
authors.forename: J M
authors.initials: JM
authors.lastname: Duderstadt
authors.forename: L W
authors.initials: LW
authors.lastname: Odioso
authors.forename: T A
authors.initials: TA
authors.lastname: Dierckman
authors.forename: W
authors.initials: W
authors.lastname: Schwecke
authors.forename: K
authors.initials: K
authors.lastname: Baran
mesh_terms: D000818:Animals; D001774:Blood Chemical Analysis / Q000662:veterinary; D001780:Blood Coagulation Tests / Q000662:veterinary; D001800:Blood Specimen Collection / Q000662:veterinary*; D006403:Hematologic Tests / Q000662:veterinary; D008297:Male; D009915:Orbit / Q000098:blood supply*; D051381:Rats; D011919:Rats, Inbred Strains; D014680:Veins; D014682:Vena Cava, Inferior