Molecular cloning and functional expression of the second mouse nm23/NDP kinase gene, nm23-M2.
A new murine cDNA of nm23/NDP kinase was isolated. A RT-PCR product was obtained from the normal mouse liver mRNA with primers designed for the human nm23-H2 gene. The product was used as a probe to screen a cDNA library from the murine melanoma cell line, B16, and two clones containing the entire open reading frame were obtained. It was predicted that the DNA sequence encoded 152 amino acids which was 98% identical to the nm23-H2 protein. The entire nm23-M1 and -M2 gene-coding regions were translated as fusion proteins with a glutathione S-transferase. These fusion proteins displayed NDP kinase activities.
Urano
T
T
Department of Oncology, Nagasaki University School of Medicine, Japan.
Takamiya
K
K
Furukawa
K
K
Shiku
H
H
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA; D051379:Mice; D008969:Molecular Sequence Data; D020559:Monomeric GTP-Binding Proteins; D054778:NM23 Nucleoside Diphosphate Kinases; D009701:Nucleoside-Diphosphate Kinase / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D011233:Precipitin Tests; D011506:Proteins / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D014157:Transcription Factors
D000911:Antibodies, Monoclonal; D054778:NM23 Nucleoside Diphosphate Kinases; D011506:Proteins; D011994:Recombinant Proteins; D014157:Transcription Factors; D004247:DNA; C517938:NME1 protein, human; C517939:Nme1 protein, mouse; D009701:Nucleoside-Diphosphate Kinase; D020559:Monomeric GTP-Binding Proteins