title: Detection and characterization of hepatitis C virus sequence in the serum of a patient with chronic HCV infection.
abstract: A cDNA fragment corresponding to the nonstructural gene region of Hepatitis C virus was cloned and sequenced. cDNA was obtained by reverse transcription of viral RNA extracted from serum of a German patient with chronic post transfusion hepatitis. "Nested" PCR resulted in a cDNA fragment of 345 nt. The sequence showed a homology of 96% to the American prototype HCV.
authors.affiliation: Robert Koch-Institute of the Federal Health Office, Berlin, Federal Republic of Germany.
authors.forename: E
authors.initials: E
authors.lastname: Schreier
authors.forename: K
authors.initials: K
authors.lastname: Fuchs
authors.forename: M
authors.initials: M
authors.lastname: Höhne
authors.forename: M
authors.initials: M
authors.lastname: Motz
authors.forename: R
authors.initials: R
authors.lastname: Zachoval
authors.forename: J
authors.initials: J
authors.lastname: Esteban
authors.forename: S
authors.initials: S
authors.lastname: Dittmann
authors.forename: F
authors.initials: F
authors.lastname: Deinhardt
authors.forename: M
authors.initials: M
authors.lastname: Roggendorf
mesh_terms: D001483:Base Sequence; D005814:Genes, Viral / Q000235:genetics*; D016174:Hepacivirus / Q000235:genetics*; D006526:Hepatitis C / Q000453:epidemiology / Q000235:genetics*; D006521:Hepatitis, Chronic / Q000453:epidemiology / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D012367:RNA, Viral / Q000097:blood*; D012689:Sequence Homology, Nucleic Acid
chemical_list: D012367:RNA, Viral