Renal beta-adrenoceptors in thyroxine-treated rats.
The number of beta-adrenoceptors was measured in cardiac and renal cortex membranes from controls and thyroxine-treated rats. Two methods of membrane preparation (sucrose- and KCl-extraction) were compared. KCl-extraction yielded a higher number of beta-adrenoceptors, accompanied by increased activity of the plasma membrane enzyme marker 5'-nucleotidase. Thyroxine increased the number of beta-adrenoceptors in both cardiac and renal cortex membranes prepared with both methods. These results suggest that: i) Enrichment of the membrane fraction may explain the higher number of beta-adrenoceptors found with KCl, ii) Thyroid hormone plays a role in the regulation of beta-adrenoceptors in the renal cortex.
Haro
J M
JM
Departamento de Bioquímica, Facultad de Medicina, Granada, Spain.
Sabio
J M
JM
Vargas
F
F
D015720:5'-Nucleotidase / Q000378:metabolism; D000818:Animals; D002458:Cell Fractionation; D002462:Cell Membrane / Q000378:metabolism; D004082:Dihydroalprenolol / Q000378:metabolism; D006980:Hyperthyroidism / Q000139:chemically induced / Q000378:metabolism; D007672:Kidney Cortex / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D009206:Myocardium / Q000378:metabolism; D011189:Potassium Chloride; D051381:Rats; D017208:Rats, Wistar; D011943:Receptors, Adrenergic, beta / Q000187:drug effects / Q000378:metabolism*; D013395:Sucrose; D013974:Thyroxine / Q000494:pharmacology*
D011943:Receptors, Adrenergic, beta; D013395:Sucrose; D004082:Dihydroalprenolol; D011189:Potassium Chloride; D015720:5'-Nucleotidase; D013974:Thyroxine