Modulation of fatty-acid-binding protein content of rat heart and skeletal muscle by endurance training and testosterone treatment.
The effects of training and/or testosterone treatment and its aromatization to oestradiol on fatty-acid-binding protein (FABP) content and cytochrome c oxidase activity in heart, soleus and extensor digitorum longus (EDL) muscles were studied in intact adult female rats. One group of rats remained sedentary, whereas the others were trained for 7 weeks. Thereafter the trained rats were divided into control and testosterone-treated groups, with or without an aromatase inhibitor. Testosterone was administered by a silastic implant. Training was continued for 2 weeks. In untreated sedentary rats the immunochemically assayed FABP contents were 497 +/- 28, 255 +/- 49 and 58 +/- 17 micrograms/g wet weight for the heart, soleus, and EDL respectively. In the heart the FABP content was increased after training (29%), testosterone treatment (33%) or both manipulations (53%). In soleus muscle FABP increased only after testosterone treatment (16%), whereas in EDL no changes were found. Inhibiting the aromatase enzyme complex abolished the testosterone-induced effect on FABP content in soleus (suggesting an oestradiol effect) but not in heart muscle. Among the three muscles studied the FABP content was found to be related to the cytochrome c oxidase activity in a non-linear way. In conclusion, it is shown that the FABP contents and mitochondrial activities of heart and skeletal muscle are affected by training and sex hormones and that these effects are different for heart and skeletal muscles.
van Breda
E
E
Department of Physiology, University of Limburg, Maastricht, The Netherlands.
Keizer
H A
HA
Vork
M M
MM
Surtel
D A
DA
de Jong
Y F
YF
van der Vusse
G J
GJ
Glatz
J F
JF
D000818:Animals; D047072:Aromatase Inhibitors; D002352:Carrier Proteins / Q000378:metabolism*; D003576:Electron Transport Complex IV / Q000378:metabolism; D004958:Estradiol / Q000378:metabolism; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D005260:Female; D006321:Heart / Q000187:drug effects; D008929:Mitochondria, Heart / Q000201:enzymology / Q000378:metabolism; D009132:Muscles / Q000187:drug effects / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism*; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D010805:Physical Conditioning, Animal; D051381:Rats; D013739:Testosterone / Q000097:blood / Q000494:pharmacology*
D047072:Aromatase Inhibitors; D002352:Carrier Proteins; C490174:Fabp7 protein, rat; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D013739:Testosterone; D004958:Estradiol; D003576:Electron Transport Complex IV