Expression of SV40 T-antigen in lipoma cells with a chromosomal translocation T(3;12) is not sufficient for direct immortalization.
12q13-15 changes are the most frequent cytogenetic abnormalities in human tumor cells. To test their biological significance we used an assay based on lipoma cells with a limited in vitro lifetime and this type of chromosomal aberration. Lipoma cells with a reciprocal translocation t(3;12)(q28;q14) were transformed by transfection with a plasmid containing the SV40 "early region". The transformed cells showed an altered morphology with loss of contact inhibition, formation of foci, and T-antigen expression. They were immortalized after a growth crisis. The karyotypic patterns before and after the crisis show that the translocation together with expression of SV40 T-antigen is not sufficient for direct immortalization.
Belge
G
G
Center of Human Genetics and Genetic Counselling, University of Bremen, F.R.G.
Kazmierczak
B
B
Meyer-Bolte
K
K
Bartnitzke
S
S
Bullerdiek
J
J
D000328:Adult; D000952:Antigens, Polyomavirus Transforming / Q000032:analysis / Q000235:genetics*; D002455:Cell Division; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral; D005260:Female; D005814:Genes, Viral; D006801:Humans; D007621:Karyotyping; D008067:Lipoma / Q000235:genetics / Q000473:pathology*; D013539:Simian virus 40 / Q000235:genetics; D014162:Transfection; D014178:Translocation, Genetic; D014407:Tumor Cells, Cultured
D000952:Antigens, Polyomavirus Transforming