[Rapid detection of anti-HAV IgM by solid-phase immunosorbent hemagglutination inhibition test].
A solid-phase immunosorbent hemagglutination inhibition test (SPISHAI) was developed for hepatitis A virus-specific immunoglobulin M (IgM) antibody. Three hundred thirty and six sera were comparatively detected with both SPISHAI and ELISA. Among them 97 sera were positive and 237 were negative with both method. The crude agreement rate was 99.4%. With SPISHAI the titers of anti-HAV IgM ranged from 1:20 to 1:327,680 among tested sera from infected individuals by HAV. The specificity of SPISHAI was confirmed by 2-ME treatment method and blocking test. The patients with non-A hepatitis all got negative results. The SPISHAI does not require conjugated antibody and sophisticated equipment, and is not interfered with rheumatoid factor in sera. Furthermore, the result of the test can be got within 3 hours. Therefore, the SPISHAI is a cheap and simple, and could be applied for early diagnosis and epidemiological surveillance of hepatitis A in the community and in primary health care.
Xian
L
L
Department of Epidemiology, Fourth Military Medical University, Xian.
D006385:Hemagglutination Inhibition Tests / Q000379:methods; D035922:Hepatitis A Antibodies; D006508:Hepatitis Antibodies / Q000097:blood*; D006507:Hepatovirus / Q000276:immunology*; D006801:Humans; D007075:Immunoglobulin M / Q000097:blood*; D007163:Immunosorbent Techniques
D035922:Hepatitis A Antibodies; D006508:Hepatitis Antibodies; D007075:Immunoglobulin M