title: Rat surfactant protein D enhances the production of oxygen radicals by rat alveolar macrophages.
abstract: Rat surfactant protein D (SP-D) was shown to enhance the production of oxygen radicals by rat alveolar macrophages. This enhancement, which was determined by a lucigenin-dependent chemiluminescence assay, was maximal after 18 min at an SP-D concentration of 0.2 micrograms/ml. Surfactant lipids did not influence the stimulation of alveolar macrophages by SP-D, whereas the oxygen-radical production of these cells induced by surfactant protein A was inhibited by the lipids in a concentration-dependent manner.
authors.affiliation: Laboratory of Veterinary Biochemistry, University of Utrecht, The Netherlands.
authors.forename: J F
authors.initials: JF
authors.lastname: Van Iwaarden
authors.forename: H
authors.initials: H
authors.lastname: Shimizu
authors.forename: P H
authors.initials: PH
authors.lastname: Van Golde
authors.forename: D R
authors.initials: DR
authors.lastname: Voelker
authors.forename: L M
authors.initials: LM
authors.lastname: Van Golde
mesh_terms: D000818:Animals; D001992:Bronchoalveolar Lavage Fluid; D006023:Glycoproteins / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D007700:Kinetics; D008163:Luminescent Measurements; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D016676:Macrophages, Alveolar / Q000187:drug effects / Q000378:metabolism*; D010743:Phospholipids / Q000494:pharmacology; D011510:Proteolipids / Q000302:isolation & purification / Q000494:pharmacology; D037663:Pulmonary Surfactant-Associated Protein D; D037661:Pulmonary Surfactant-Associated Proteins; D011663:Pulmonary Surfactants / Q000302:isolation & purification / Q000494:pharmacology*; D051381:Rats; D013481:Superoxides / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
chemical_list: D006023:Glycoproteins; D010743:Phospholipids; D011510:Proteolipids; D037663:Pulmonary Surfactant-Associated Protein D; D037661:Pulmonary Surfactant-Associated Proteins; D011663:Pulmonary Surfactants; D013481:Superoxides; D013755:Tetradecanoylphorbol Acetate