Solid-phase synthesis of an RNA nucleopeptide fragment from the nucleoprotein of poliovirus.
The naturally occurring RNA-nucleopeptide H-Ala-Tyr[5'-pUUAAAAC-3']-NH2 is prepared via a solid-phase phosphite triester approach using N-SiOMB/O-TBDMS-protected nucleosides. Preliminary 1H-NMR studies show that the peptidyl unit has a remarkable effect on the conformational behaviour of the RNA moiety in the nucleopeptide.
Dreef-Tromp
C M
CM
Gorlaeus Laboratory, Leiden, The Netherlands.
van den Elst
H
H
van den Boogaart
J E
JE
van der Marel
G A
GA
van Boom
J H
JH
D009682:Magnetic Resonance Spectroscopy; D009690:Nucleic Acid Conformation; D009698:Nucleoproteins / Q000737:chemistry; D009843:Oligoribonucleotides / Q000737:chemistry*; D010446:Peptide Fragments / Q000138:chemical synthesis* / Q000737:chemistry; D017955:Poliovirus / Q000737:chemistry*; D012367:RNA, Viral / Q000737:chemistry*; D012261:Ribonucleoproteins / Q000138:chemical synthesis* / Q000737:chemistry; D014764:Viral Proteins / Q000138:chemical synthesis* / Q000737:chemistry
D009698:Nucleoproteins; D009843:Oligoribonucleotides; D010446:Peptide Fragments; D012367:RNA, Viral; D012261:Ribonucleoproteins; D014764:Viral Proteins