Identification of a Drosophila gene encoding a calmodulin-binding protein with homology to the trp phototransduction gene.
We have isolated a number of Drosophila cDNAs on the basis of their encoding calmodulin-binding proteins. A full-length cDNA clone corresponding to one of these genes has been cloned and sequenced. Conservation of amino acid sequence and tissue-specific expression are observed between this gene and the transient receptor potential (trp) gene. We propose the name transient receptor potential-like (trpl) to describe this newly isolated gene. The trpl protein contains two possible calmodulin-binding sites, six transmembrane regions, and a sequence homologous to an ankyrin-like repeat. Structurally, the trpl and trp proteins resemble cation channel proteins, particularly the brain isoform of the voltage-sensitive Ca2+ channel. The identification of a protein similar to the trp gene product, yet also able to bind Ca2+/calmodulin, allows for a reinterpretation of the phenotype of the trp mutations and suggests that both genes may encode light-sensitive ion channels.
Phillips
A M
AM
Department of Genetics, University of Melbourne, Parkville, Victoria, Australia.
Bull
A
A
Kelly
L E
LE
D000595:Amino Acid Sequence; D000818:Animals; D017487:Ankyrins; D001798:Blood Proteins / Q000737:chemistry; D015153:Blotting, Western; D015220:Calcium Channels / Q000737:chemistry; D002147:Calmodulin / Q000378:metabolism; D002148:Calmodulin-Binding Proteins / Q000737:chemistry / Q000235:genetics*; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000235:genetics*; D015870:Gene Expression; D005796:Genes; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010786:Photoreceptor Cells / Q000502:physiology*; D011487:Protein Conformation; D016415:Sequence Alignment; D050051:Transient Receptor Potential Channels
D017487:Ankyrins; D001798:Blood Proteins; D015220:Calcium Channels; D002147:Calmodulin; D002148:Calmodulin-Binding Proteins; D029721:Drosophila Proteins; D008565:Membrane Proteins; D050051:Transient Receptor Potential Channels; C074192:trpl protein, Drosophila; D004247:DNA