Regional glycine receptor binding in the p,p'-DDT myoclonic rat model.
Abnormal glycinergic neurotransmission has been implicated in the pathophysiology of DDT-induced myoclonus. To examine the role of glycine receptors in the DDT model, was measured [3H]strychnine receptor binding in brainstem and spinal cord in the rat after acute administration of DDT. The highest dose of DDT tested significantly increased both Bmax (20%) and Kd (57%) of glycine sites in spinal cord but not brainstem compared to vehicle-treated controls at 4 h. Lower DDT doses, which also induced myoclonus, had no significant effects on [3H]strychnine specific binding. In vitro, 10(-7) DDT did not displace [3H]strychnine binding in naive rat spinal cord, but higher doses could not be studied due to poor solubility of DDT under the assay conditions. These data suggest that only a maximal dose of DDT has significant though mixed effects on parameters of [3H]strychnine binding in spinal cord which are not correlated with the onset of myoclonus.
Pranzatelli
M R
MR
Department of Neurology, George Washington University, Washington, DC.
Tkach
K
K
D000818:Animals; D001933:Brain Stem / Q000378:metabolism; D003634:DDT / Q000633:toxicity*; D004195:Disease Models, Animal; D008297:Male; D009207:Myoclonus / Q000139:chemically induced*; D051381:Rats; D011919:Rats, Inbred Strains; D018009:Receptors, Glycine; D017981:Receptors, Neurotransmitter / Q000187:drug effects* / Q000378:metabolism; D013116:Spinal Cord / Q000378:metabolism; D013331:Strychnine / Q000378:metabolism
D018009:Receptors, Glycine; D017981:Receptors, Neurotransmitter; D003634:DDT; D013331:Strychnine