Evidence for an internal pool of nucleoside transporters in mammalian reticulocytes.
Comparisons of the site specific binding of nitrobenzylthioinosine (NBMPR) to intact and lysed red cells from various mammalian and avian species suggest the presence of a cytoplasmic pool of nucleoside transporters. In some species the cytoplasmic pool is about 50% of the total (mouse). On the average, the cytoplasmic pool is approx. 20% of the surface pool of NBMPR-binding sites. In sheep reticulocytes, both pools disappear in an energy-dependent manner during the maturation of the reticulocyte in vitro.
Liang
L
L
Department of Biochemistry, McGill University, Montreal, Canada.
Johnstone
R M
RM
D002731:4-Chloromercuribenzenesulfonate / Q000494:pharmacology; D000345:Affinity Labels; D000818:Animals; D001665:Binding Sites; D001798:Blood Proteins / Q000378:metabolism; D002352:Carrier Proteins / Q000378:metabolism*; D002478:Cells, Cultured; D002642:Chick Embryo; D004910:Erythrocyte Membrane / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism*; D009245:NADH Dehydrogenase / Q000378:metabolism; D033703:Nucleoside Transport Proteins; D012156:Reticulocytes / Q000378:metabolism*; D012756:Sheep; D013868:Thioinosine / Q000031:analogs & derivatives / Q000378:metabolism; D014357:Trypsin / Q000378:metabolism
D000345:Affinity Labels; D001798:Blood Proteins; D002352:Carrier Proteins; D008565:Membrane Proteins; D033703:Nucleoside Transport Proteins; D013868:Thioinosine; D002731:4-Chloromercuribenzenesulfonate; D009245:NADH Dehydrogenase; D014357:Trypsin; C001789:4-nitrobenzylthioinosine