Structural alterations of the ribosomal RNA genes in leukemic cells.
Cloned 6.7 kb EcoR1 fragment of mice rDNA was used as a hybridization probe for rDNA structure analysis in mice, rat and calf haemopoietic tumor and normal cells. EcoR1, BglII and Pst1 restriction fragment length polymorphism (RFLP) was found in neoplastic rDNA and was not revealed in normal ones. The rRNA gene rearrangements were observed not only in spacer region but in coding sequences of the genes. Leukemic cells reveal also rDNA amplification. A role of genetic rearrangements of rDNA for mechanisms of carcinogenesis is suggested.
Smirnova
I A
IA
Institute of Experimental Pathology, Oncology and Radiobiology, Ukranian Academy of Sciences, Kiev.
D000818:Animals; D002471:Cell Transformation, Neoplastic / Q000235:genetics; D002472:Cell Transformation, Viral / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005260:Female; D005784:Gene Amplification / Q000235:genetics; D015321:Gene Rearrangement / Q000235:genetics*; D006413:Hematopoietic System / Q000166:cytology / Q000502:physiology; D007938:Leukemia / Q000235:genetics* / Q000382:microbiology; D004915:Leukemia, Erythroblastic, Acute / Q000235:genetics; D008297:Male; D008745:Methylation; D051379:Mice; D008815:Mice, Inbred Strains; D009693:Nucleic Acid Hybridization; D012334:RNA, Neoplasm / Q000235:genetics* / Q000378:metabolism; D012335:RNA, Ribosomal / Q000235:genetics* / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D011925:Rauscher Virus; D014412:Tumor Virus Infections / Q000235:genetics
D004275:DNA, Ribosomal; D012334:RNA, Neoplasm; D012335:RNA, Ribosomal