title: Epstein-Barr virus (EBV) genomes and c-myc oncogene in oral Burkitt's lymphomas.
abstract: In addition to Burkitt's lymphomas, tentative evidence suggests the involvement of Epstein-Barr virus (EBV) in malignant lymphomas of T-cell origin. The c-myc proto-oncogene is strongly associated with the development of lymphoid neoplasias. In the present study, a series of 38 biopsies of oral lymphomas (29 Burkitt's lymphomas, 9 malignant lymphomas of other type) obtained from patients in Tanzania were studied using in situ hybridization (ISH) and polymerase chain reaction (PCR) for detection of EBV DNA and c-myc oncogene. In ISH applied on formalin-fixed, paraffin wax-embedded biopsies, the Bam HI W fragment of EBV DNA was used as the probe. Amplification of c-myc oncogene was studied by PCR with a primer set from Exon II area. As an internal standard beta-globin gene was simultaneously amplified. EBV DNA was disclosed by ISH in five Burkitt's lymphomas only. Using the PCR, 20 of the 29 cases (70%) of Burkitt's lymphomas showed amplification for EBV DNA. Of the other EBV-positive lymphomas, two were of the lymphocytic type (large non-cleaved cell), one histiocytic and one Burkitt's-like lymphoma. All EBV-positive cases found on the agarose gel were positive also with the dot blot, when hybridized with the 32P-labeled EBV Bam HI W-fragment probe. All lymphomas showed similar bands on the gel for c-myc and beta-globin indicating that no amplification of c-myc was present.
authors.affiliation: Department of Pathology, University of Kuopio, Finland.
authors.forename: S
authors.initials: S
authors.lastname: Syrjänen
authors.forename: P
authors.initials: P
authors.lastname: Kallio
authors.forename: P
authors.initials: P
authors.lastname: Sainio
authors.forename: C
authors.initials: C
authors.lastname: Fuju
authors.forename: K
authors.initials: K
authors.lastname: Syrjänen
mesh_terms: D002051:Burkitt Lymphoma / Q000235:genetics* / Q000382:microbiology*; D004279:DNA, Viral / Q000235:genetics; D005784:Gene Amplification; D016259:Genes, myc / Q000235:genetics*; D016679:Genome, Viral; D004854:Herpesvirus 4, Human / Q000235:genetics*; D006801:Humans; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000235:genetics / Q000382:microbiology; D007972:Leukoplakia, Oral / Q000235:genetics / Q000382:microbiology; D016393:Lymphoma, B-Cell / Q000235:genetics* / Q000382:microbiology*; D016403:Lymphoma, Large B-Cell, Diffuse / Q000235:genetics / Q000382:microbiology; D009062:Mouth Neoplasms / Q000235:genetics* / Q000382:microbiology*; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D000090063:Proto-Oncogene Mas; D013636:Tanzania
chemical_list: D004279:DNA, Viral; C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas