Activation of human eosinophils in vitro by respiratory syncytial virus.
To determine the nature of the interaction between viruses and eosinophils, normodense eosinophils were separated from the blood of healthy volunteers and incubated in vitro with respiratory syncytial virus (RSV). After incubation for 2 h with the virus, 29.5 +/- 15.8% of the eosinophils demonstrated specific binding of the virus to the cell membrane, as detected by fluorescent staining with an anti-RSV MAb. Superoxide production and leukotriene C4 release were measured as determinants of cell activation. Using a cytochrome c reduction assay, superoxide could be detected in the supernatant 30 min after exposure to RSV. Maximal release was reached at 3 h postexposure (5.88 +/- 2.19 nmol cytochrome c reduction/5 x 10(5) cells). The virus-induced superoxide generation varied in magnitude among different subjects and ranged from 0.6 to 11.5 nmol cytochrome c reduction/5 x 10(5) cells. RSV also appeared to prime eosinophils to the effects of other known cell activators, as demonstrated by an increase in superoxide production upon subsequent stimulation of RSV-primed cells with phorbol-12-myristate-13-acetate (21.4 +/- 5.8 versus 9.4 +/- 2.7 nmol cytochrome c reduction/5 x 10(5) cells for primed and unprimed cells, respectively) (p less than 0.04). RSV did not directly induce leukotriene C4 release from the eosinophils but primed the cells to exhibit a more vigorous response on subsequent challenge with the calcium ionophore A23187 (9.16 +/- 1.04 versus 4.2 +/- 1.3 ng leukotriene C4/1 x 10(6) cells) (p less than 0.005). These findings indicate that RSV can activate or prime eosinophils to release various inflammatory mediators.(ABSTRACT TRUNCATED AT 250 WORDS)
Kimpen
J L
JL
School of Medicine, State University of New York, Buffalo.
Garofalo
R
R
Welliver
R C
RC
Ogra
P L
PL
D000328:Adult; D001988:Bronchiolitis / Q000209:etiology; D004804:Eosinophils / Q000502:physiology*; D006801:Humans; D066298:In Vitro Techniques; D012136:Respiratory Syncytial Viruses / Q000472:pathogenicity*; D010253:Respirovirus Infections / Q000209:etiology; D013189:SRS-A / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism
D013189:SRS-A; D013481:Superoxides