title: Characterization of monoclonal antibodies to the outer membrane protein (OmpD) of Salmonella typhimurium.
abstract: A panel of monoclonal antibodies, seven against the trimeric and seven against the monomeric forms to outer membrane protein D (OmpD) of Salmonella typhimurium were produced. The specificities of these monoclonal antibodies for the porin proteins of S. typhimurium and their cross-reactions with Salmonella porins OmpC and OmpF were determined by Western immunoblotting and enzyme-linked immunosorbent assay. We observed that OmpD shared more epitopes and had greater structural similarity with OmpC than with OmpF.
authors.affiliation: Biomedical Research Program, Alabama State University, Montgomery 36101.
authors.forename: S R
authors.initials: SR
authors.lastname: Pai
authors.forename: Y
authors.initials: Y
authors.lastname: Upshaw
authors.forename: S P
authors.initials: SP
authors.lastname: Singh
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry / Q000276:immunology*; D003429:Cross Reactions; D051379:Mice; D018272:Porins; D011487:Protein Conformation; D012486:Salmonella typhimurium / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D018272:Porins