Endocarditis caused by Candida parapsilosis.
The authors report a case of endocarditis caused by Candida parapsilosis. To the best of our knowledge, a case has not been described previously in Japan in the English literature. A battery of 8 peroxidase-labeled lectins was tested on sections of paraffin-embedded tissue to determine which lectin could be used in the microscopic diagnosis of C. parapsilosis. One lectin, from Archis hypoaea (PNA) was found to react with C. parapsilosis. On the other hand, C. albicans, Aspergillus, Mucor, and Cryptococcus did not react with A. hypoaea (PNA). On fluorescence microscopic study, C. parapsilosis was not fluorescent, but other fungi were fluorescent when exposed to ultraviolet illumination. Therefore, we propose new procedures for identification of C. parapsilosis in tissue sections using lectin histochemistry and fluorescence microscopy.
Senba
M
M
Department of Pathology, Nagasaki University, Japan.
Watanabe
K
K
Yoshida
K
K
Ochi
S
S
Matsumoto
K
K
Yano
K
K
Itakura
H
H
D002177:Candidiasis / Q000150:complications* / Q000175:diagnosis / Q000382:microbiology; D003937:Diagnosis, Differential; D004452:Echocardiography; D004696:Endocarditis / Q000000981:diagnostic imaging / Q000209:etiology / Q000382:microbiology*; D016469:Fungemia / Q000150:complications* / Q000175:diagnosis / Q000382:microbiology; D006801:Humans; D008297:Male; D008875:Middle Aged; D010504:Periodic Acid; D013438:Sulfhydryl Compounds
D013438:Sulfhydryl Compounds; D010504:Periodic Acid; C005732:thioacetic acid