title: Accumulation of the pre-assembled membrane arm of NADH:ubiquinone oxidoreductase in mitochondria of manganese-limited grown Neurospora crassa.
abstract: The NADH:ubiquinone oxidoreductase (complex I) of mitochondria is constructed from two arms arranged perpendicular to each other. The peripheral arm protruding into the matrix contains the proximal section of the electron pathway, and the membrane arm with all mitochondrially encoded subunits contains the distal section of the electron pathway. When Neurospora crassa is grown under manganese limitation the formation of the peripheral arm is disturbed, but the membrane arm containing the iron-sulfur cluster N-2, is accumulated. An extra-polypeptide, assumed to be a chaperone, is found to be associated with this pre-assembled membrane arm.
authors.affiliation: Institut für Biochemie, Universität Düsseldorf, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Schmidt
authors.forename: T
authors.initials: T
authors.lastname: Friedrich
authors.forename: J
authors.initials: J
authors.lastname: Wallrath
authors.forename: T
authors.initials: T
authors.lastname: Ohnishi
authors.forename: H
authors.initials: H
authors.lastname: Weiss
mesh_terms: D004578:Electron Spin Resonance Spectroscopy; D007425:Intracellular Membranes / Q000201:enzymology; D007506:Iron-Sulfur Proteins / Q000378:metabolism; D008345:Manganese / Q000378:metabolism*; D008928:Mitochondria / Q000201:enzymology*; D016660:NAD(P)H Dehydrogenase (Quinone) / Q000378:metabolism*; D009492:Neurospora crassa / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development; D011233:Precipitin Tests; D011487:Protein Conformation
chemical_list: D007506:Iron-Sulfur Proteins; D008345:Manganese; D016660:NAD(P)H Dehydrogenase (Quinone)