Canatoxin induces activation on mice peritoneal macrophages.
Canatoxin (CNTX), the toxic protein from Canavalia ensiformis seeds, injected into the peritoneal cavities of mice (10 micrograms/cavity) induced a significant neutrophil migration (10.5 +/- 0.5 x 10(6) cells/cavity) after 4 h. A later migratory effect (48 h) on mononuclear cells, predominantly macrophages, was also observed (controls: 7 +/- 0.9; CNTX: 17 +/- 2.0 x 10(6) cells/cavity). These CNTX-elicited macrophages, when compared to resident cells (R) or cells elicited by thioglycollate (TG), had an increased content of the lysosomal enzyme N-acetyl-beta-D-glucosaminidase (R: 4.5 +/- 0.5; TG: 7.2 +/- 1.0; CNTX: 20.2 +/- 3.0 mU/10(6) cells) and a greater (> or = 100%) phagocytic activity. The data suggest that CNTX-stimulated macrophages presented some characteristics of activated cells.
Ghazaleh
F A
FA
Departamento de Bioquímica Médica, Universidade Federal do Rio de Janeiro, Brasil.
Araújo
C F
CF
Barja-Fidalgo
C
C
Carlini
C R
CR
D000118:Acetylglucosaminidase / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D037102:Lectins / Q000494:pharmacology*; D008247:Lysosomes / Q000187:drug effects / Q000201:enzymology; D008262:Macrophage Activation / Q000187:drug effects*; D017737:Macrophages, Peritoneal / Q000187:drug effects* / Q000201:enzymology / Q000276:immunology; D051379:Mice; D010587:Phagocytosis / Q000187:drug effects; D010940:Plant Proteins; D013864:Thioglycolates / Q000494:pharmacology; D013997:Time Factors; D014118:Toxins, Biological / Q000494:pharmacology*
D037102:Lectins; D010940:Plant Proteins; D013864:Thioglycolates; D014118:Toxins, Biological; C032815:canatoxin; D000118:Acetylglucosaminidase