[The early diagnosis of leptospirosis by demonstrating antigens via an immunohistochemical exam of the hamstring muscle].
Establishing an early and certain diagnosis of leptospirosis has been a difficult task. So, we analysed the histopathological alterations of the gastrocnemius muscle, and studied, for the first time, the usefulness of the immune histochemical method of peroxidase-antiperoxidase for the demonstration of the spirochete and of its products in this tissue. Histopathological observations have shown a picture of myositis, characterized by interstitial inflammatory infiltrate and necrotic-degenerative abnormalities of muscle fibers. The lesions were considered minimal in 69.45% of the patients, moderate in 19.45%, severe in 5.55% and absent in the remaining. On the other hand, the immune histochemical method identified the etiology in 94.45% what was considered very expressive.
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Divisão de Clínica de Moléstias Infecciosas e Parasitárias, Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo.
Amato Neto
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Duarte
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D000293:Adolescent; D000328:Adult; D000368:Aged; D000942:Antigens, Bacterial / Q000378:metabolism*; D001706:Biopsy; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007921:Leptospira interrogans / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D009132:Muscles / Q000378:metabolism* / Q000473:pathology; D009220:Myositis / Q000175:diagnosis / Q000473:pathology; D013997:Time Factors; D014895:Weil Disease / Q000175:diagnosis* / Q000473:pathology
D000942:Antigens, Bacterial
Diagnóstico precoce da leptospirose por demonstração de antígenos através de exame imuno-histoquímico em músculo da panturrilha.