[Hepatitis B virus infection in monocytes and their functional changes in patients with chronic hepatitis B].
We detected the HBV DNA in peripheral blood monocytes of 70 patients with chronic hepatitis B and 20 healthy controls by polymerase chain reaction (PCR) and Southern transfer hybridization technique, the HLA-DR expression on monocytic membrane and tumor necrosis factor-alpha (TNF alpha) content and interleukin 1 (IL 1) activity in their monocytic culture supernatant were also measured respectively by indirect immunofluorescence technique, ELISA, and thymocytic multiplication assay. HBV DNA sequences were detected in monocytes of 42(60%) of the 70 patients. The percentage of monocyte-expressing HLADR and the TNF alpha content in monocyte culture supernatant were significantly elevated in patients with HBV DNA-positive monocytes compared with those patients with HBV-DNA-negative monocytes. The percentage of monocyte-expressing HLA-DA and the level of TNF alpha produced by monocytes correlated markedly with the intensity of HBV DNA-positive signal in monocytes. These data suggested that HBV infection in monocytes might cause a series of functional changes in these cells.
Guang
L X
LX
Department of Infectious Disease, Southwest Hospital, Third Military Medical College, Chongqing.
D000293:Adolescent; D000328:Adult; D004279:DNA, Viral / Q000097:blood; D005260:Female; D006684:HLA-DR Antigens / Q000097:blood; D006509:Hepatitis B / Q000276:immunology* / Q000382:microbiology; D006515:Hepatitis B virus / Q000235:genetics / Q000302:isolation & purification*; D006521:Hepatitis, Chronic / Q000276:immunology / Q000382:microbiology; D006801:Humans; D007375:Interleukin-1 / Q000097:blood; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000276:immunology* / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis
D004279:DNA, Viral; D006684:HLA-DR Antigens; D007375:Interleukin-1; D014409:Tumor Necrosis Factor-alpha