Alterations within pp59v-rel-containing protein complexes following the stimulation of REV-T-transformed lymphoid cells with zinc.
pp59v-rel exists in association with specific cellular proteins within lymphoid cells transformed by reticuloendotheliosis virus (REV-T). These include the cellular rel homolog (p75c-rel) as well as a 40-kDa avian homolog to I kappa B. The brief exposure of REV-T-transformed lymphoid cells to micromolar concentrations of ZnSO4 induces profound alterations within these protein complexes. Most of the constituents of the rel protein complexes (to include pp59v-rel, p75c-rel, and p115) translocate from the cytosol to the nucleus. This system has been used to characterize the molecular events that accompany the activation of rel protein complexes. The level of phosphorylation increases on three proteins within these complexes: pp59v-rel, p75-c-rel, and pp40. The degree of phosphorylation on pp59v-rel is such that its relative mass increases 3 to 6 kDa when resolved by SDS-polyacrylamide gel electrophoresis. pp59v-rel is phosphorylated on serine and threonine residues predominantly within a single domain of 17.5 kDa. Similarly, p75c-rel exhibits a corresponding increase in its relative mass with increased phosphorylation. The increased phosphorylation of pp40 is accompanied by its dissociation from the cytosolic rel protein complexes. These observations draw parallels with the induction of the NF-kappa B trans-activating factor.
Storms
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Department of Microbiology, University of Texas, Austin 78712-1095.
Bose
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D000470:Alkaloids / Q000494:pharmacology; D002104:Cadmium / Q000494:pharmacology; D002467:Cell Nucleus / Q000378:metabolism; D002472:Cell Transformation, Viral; D004789:Enzyme Activation / Q000187:drug effects; D046911:Macromolecular Substances; D009687:Nuclear Proteins / Q000378:metabolism; D020572:Oncogene Proteins v-rel; D010750:Phosphoproteins / Q000378:metabolism; D010766:Phosphorylation; D047428:Protein Kinase Inhibitors; D011494:Protein Kinases / Q000378:metabolism*; D001356:Reticuloendotheliosis virus; D015682:Retroviridae Proteins, Oncogenic / Q000378:metabolism*; D019311:Staurosporine; D015032:Zinc / Q000494:pharmacology*
D000470:Alkaloids; D046911:Macromolecular Substances; D009687:Nuclear Proteins; D020572:Oncogene Proteins v-rel; D010750:Phosphoproteins; D047428:Protein Kinase Inhibitors; D015682:Retroviridae Proteins, Oncogenic; D002104:Cadmium; D011494:Protein Kinases; D019311:Staurosporine; D015032:Zinc