title: [Dalargin-binding proteins of synaptic membranes from the rat brain: isolation and comparison of their properties with opiate receptor properties].
abstract: The proteins isolated from rat brain synaptic membranes were studied by affinity chromatography on dalargin-omega-aminohexyl-Sepharose 4B and specific elution with DAGO (Tyr-D-Ala-Gly-N-Me-Gly-ol). These proteins were shown to bind specifically 3H-naloxone (Kd = 6.6 nM; Bmax = 690 pmol/mg of protein). SDS electrophoresis of the dalargin-binding proteins termed as DBPDAGO revealed one major protein band with M(r) of 42 kDa and two minor bands with M(r) of 29 and 67 kDa. The glycoprotein component was found in DBPDAGO; their isoelectric properties were established (pI 5.4). The close similarity of DBP properties with those of isolated brain opiate receptors suggest them to be opiate receptor components.
authors.forename: G A
authors.initials: GA
authors.lastname: Izykenova
authors.forename: N P
authors.initials: NP
authors.lastname: Taranova
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000187:drug effects*; D002853:Chromatography, Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D016308:Enkephalin, Leucine-2-Alanine / Q000031:analogs & derivatives* / Q000494:pharmacology; D007525:Isoelectric Focusing; D008297:Male; D008969:Molecular Sequence Data; D009020:Morphine / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D011957:Receptors, Opioid / Q000378:metabolism*; D013570:Synaptic Membranes / Q000187:drug effects*; D001615:beta-Endorphin / Q000378:metabolism
chemical_list: D009419:Nerve Tissue Proteins; D011957:Receptors, Opioid; D001615:beta-Endorphin; D016308:Enkephalin, Leucine-2-Alanine; D009020:Morphine; C040996:enkephalin-Leu, Ala(2)-Arg(6)-
vernacular_title: Dalarginsviazyvaiushchie belki sinapticheskikh membran mozga krys: vydelenie i sravnenie ikh svoĭstv so svoĭstvami opiatnykh retseptorov.