Crystallization and preliminary X-ray analysis of three serotypes of foot-and-mouth disease virus.
Foot-and-mouth disease viruses from serotypes O, A and C have been crystallized. The particular strains studied include O1K, A10(61), A22 Iraq 24/64, A24 Cruzeiro and C-S8c1. In addition, crystals have been grown of G67, a monoclonal antibody neutralization escape mutant derived from O1K, and of virus R100, recovered after the establishment of a persistent infection in baby hamster kidney cells with C-S8c1. Empty particles, capsids which lack the RNA genome, have also been crystallized for subtypes A22 Iraq 24/64 and A10(61). In almost all cases, crystals suitable for high resolution structure determination were obtained from (NH4)2SO4 or mixtures of polyethylene glycol and NH4Cl.
Curry
S
S
AFRC Institute for Animal Health, Pirbright Laboratory, Woking, U.K.
Abu-Ghazaleh
R
R
Blakemore
W
W
Fry
E
E
Jackson
T
T
King
A
A
Lea
S
S
Logan
D
D
Newman
J
J
Stuart
D
D
D005537:Aphthovirus / Q000737:chemistry* / Q000235:genetics / Q000648:ultrastructure; D002213:Capsid / Q000737:chemistry / Q000648:ultrastructure; D003460:Crystallization; D014644:Genetic Variation; D009154:Mutation; D012703:Serotyping; D014357:Trypsin / Q000494:pharmacology; D014771:Virion / Q000737:chemistry / Q000187:drug effects / Q000235:genetics / Q000648:ultrastructure; D014961:X-Ray Diffraction
D014357:Trypsin