title: A study of hydrogen exchange of monoclonal antibodies: specificity of the antigen-binding induced conformational stabilization.
abstract: Amide-hydrogen exchange of three anti-yeast iso-1-cytochrome-c IgG monoclonal antibodies and the Fab, prepared from one of them, were studied by infrared spectrophotometry in the presence and absence of the deuterated immunogen and evolutionarily related species (the deuterated immunogen contained a population of a dimer. Each subunit of the dimer appeared to bind to the antibodies in a manner similar to the monomer). The number of hydrogens of the antibodies whose exchange was suppressed on binding to the immunogen was found to exceed that estimated for the residues shielded by the immunogen. Analysis of the data suggests that such suppression of hydrogen exchange occurs mainly for the Fab domains, but not for the Fc. One of the antibodies showed two distinct classes of amide-hydrogens. Class-1 hydrogens (approx. 36/site) exchange faster than class 2 (approx. 37/site). The exchange of class-1 hydrogens was suppressed by binding to the immunogen, but not to the evolutionarily related species. The exchange of class-2 hydrogens was suppressed by binding to the evolutionarily related species, as well as to the immunogen. Thus, the suppression of exchange of class-1 hydrogens appears to occur by some kind of conformational stabilization, the mechanism of which differentiates between the deuterated immunogen and the evolutionarily related species. Evidence suggests that the trans-interactions of the Fab domains may modulate the hydrogen exchange. If it is assumed that the antigen-binding strengthens the trans-interactions in such a way that the exchange of the slower exchanging hydrogens is suppressed, this could explain the suppression of exchange of class-2 hydrogens.
authors.affiliation: Laboratory of Chemical Biology, National Institutes of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892.
authors.forename: P
authors.initials: P
authors.lastname: Rizzo
authors.forename: C
authors.initials: C
authors.lastname: Tinello
authors.forename: A
authors.initials: A
authors.lastname: Punturieri
authors.forename: H
authors.initials: H
authors.lastname: Taniuchi
mesh_terms: D000577:Amides / Q000737:chemistry; D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry* / Q000276:immunology; D000937:Antigen-Antibody Reactions; D005075:Biological Evolution; D003574:Cytochrome c Group / Q000276:immunology; D045304:Cytochromes c; D003903:Deuterium; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006825:Hybridomas; D006859:Hydrogen / Q000737:chemistry*; D007140:Immunoglobulin Fab Fragments / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D011487:Protein Conformation; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D029701:Saccharomyces cerevisiae Proteins; D013055:Spectrophotometry, Infrared
chemical_list: D000577:Amides; D000911:Antibodies, Monoclonal; C014059:CYC1 protein, S cerevisiae; D003574:Cytochrome c Group; D007140:Immunoglobulin Fab Fragments; D029701:Saccharomyces cerevisiae Proteins; D006859:Hydrogen; D045304:Cytochromes c; D003903:Deuterium