title: Evidence for polynuclear aggregates of ferric daunomycin. A Mössbauer, EPR, X-ray absorption spectroscopy and magnetic susceptibility study.
abstract: The interaction of the antitumor agent daunomycin (DN) with ferric iron has been analysed by Mössbauer spectroscopy, EPR, extended X-ray absorption fine structure (EXAFS), and magnetic susceptibility measurements. In contrast to literature data, at millimolar iron and anthracycline concentrations no solitary Fe(DN)3 complexes are formed in appreciable amounts. The Mössbauer spectroscopic analysis revealed severe dependencies on temperature, on the preparation procedure, the time allowed for equilibration, and on the metal/ligand ratio. The Mössbauer spectra exhibit two components: a broad magnetic sextet and a quadrupole doublet at an Fe/DN molar ratio of 1:3 and exclusively a doublet at a molar ratio of 1:20, indicating an equilibrium of these two spectral components. The EPR spectra are dominated by a signal at g(eff) = 2. Double integration of the EPR signals enabled the determination of their spin density and a correlation between EPR and Mössbauer spectra. The Mössbauer sextet species is EPR invisible and corresponds to magnetically ordered polynuclear aggregates with high magnetic anisotropy. EXAFS and susceptibility measurements provide additional evidence for the formation of polynuclear aggregates of ferric daunomycin. The quadrupole doublet species in the Mössbauer spectra correlates with the g = 2 signal in EPR. This species is also related to a magnetically ordered system, exhibiting, however, superparamagnetic behavior due to less magnetic anisotropy. Since daunomycin forms dimers in aqueous solution at millimolar concentrations, we conclude that the cooperative phenomena observed in EPR and Mössbauer spectra are a consequence of its stacking effects.
authors.affiliation: Mikrobiologie/Biotechnologie, Eberhard-Karls-Universität, Tübingen, Federal Republic of Germany.
authors.forename: B F
authors.initials: BF
authors.lastname: Matzanke
authors.forename: E
authors.initials: E
authors.lastname: Bill
authors.forename: C
authors.initials: C
authors.lastname: Butzlaff
authors.forename: A X
authors.initials: AX
authors.lastname: Trautwein
authors.forename: H
authors.initials: H
authors.lastname: Winkler
authors.forename: C
authors.initials: C
authors.lastname: Hermes
authors.forename: H F
authors.initials: HF
authors.lastname: Nolting
authors.forename: R
authors.initials: R
authors.lastname: Barbieri
authors.forename: U
authors.initials: U
authors.lastname: Russo
mesh_terms: D003630:Daunorubicin / Q000737:chemistry*; D004578:Electron Spin Resonance Spectroscopy; D005290:Ferric Compounds; D008280:Magnetics; D015204:Spectroscopy, Mossbauer; D013057:Spectrum Analysis; D014965:X-Rays
chemical_list: D005290:Ferric Compounds; D003630:Daunorubicin