Abnormal regulation of the myc gene in myeloid leukemia.
To study the regulation of expression of the myc protooncogene, cells from normal individuals and patients with acute myelogenous leukemia (AML), and chronic phase and blastic crisis of chronic myeloid leukemia (CML) cells were put in overnight culture in the presence or absence of fetal calf serum. Myc expression in normal marrow cells and chronic phase CML cells fell after culture in vitro. In contrast, myc expression was maintained or increased in a majority of the AML and blastic crisis CML specimens. These data demonstrate that the regulation of myc expression is disordered in many AML and blastic crisis specimens but not in chronic phase CML cells.
Gopal
V
V
University of Cincinnati Medical Center, OH 45267-0508.
Kadam
P
P
Preisler
H
H
Hulette
B
B
Li
Y Q
YQ
Steele
P
P
Freeman
J
J
Banavali
S
S
D001483:Base Sequence; D001752:Blast Crisis / Q000235:genetics*; D015139:Blotting, Southern; D001854:Bone Marrow Cells; D002452:Cell Count; D015973:Gene Expression Regulation, Leukemic / Q000187:drug effects / Q000235:genetics*; D016259:Genes, myc / Q000187:drug effects / Q000235:genetics*; D011905:Genes, ras / Q000235:genetics; D006801:Humans; D000077190:Interferon alpha-2; D016898:Interferon-alpha / Q000494:pharmacology; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics* / Q000473:pathology*; D015470:Leukemia, Myeloid, Acute / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D012334:RNA, Neoplasm / Q000235:genetics; D011994:Recombinant Proteins; D014212:Tretinoin / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D000077190:Interferon alpha-2; D016898:Interferon-alpha; D012334:RNA, Neoplasm; D011994:Recombinant Proteins; D014212:Tretinoin