Distribution and localization of Epstein-Barr virus subtypes A and B in AIDS-related lymphomas and lymphatic tissue of HIV-positive patients.
Tissue samples of 21 HIV-positive patients have been studied for the presence and distribution of Epstein-Barr virus (EBV) subtypes A and B. This was done by PCR, EBER in situ hybridization, and immunohistochemical detection of EBV latent membrane protein (LMP) in AIDS-associated malignant lymphomas (16 cases) and lymphatic organs of patients without lymphoma (5 cases). Eleven cases were considered to be EBV-positive, with type A in four, and type B virus in four other cases. In patients with malignant lymphoma, EBV was localized in tumour tissue exclusively. One patient without lymphoma presented with multiple EBV genome type B-positive cells in all the lymphoid tissue samples examined. In HIV-positive patients, both subtypes of EBV, A and B, may play a role in the pathogenesis of lymphoproliferative lesions.
Borisch
B
B
Institute of Pathology, Bern, Switzerland.
Finke
J
J
Hennig
I
I
Delacrétaz
F
F
Schneider
J
J
Heitz
P U
PU
Laissue
J A
JA
D000328:Adult; D000368:Aged; D005260:Female; D015658:HIV Infections / Q000382:microbiology*; D004854:Herpesvirus 4, Human / Q000145:classification / Q000302:isolation & purification*; D006801:Humans; D017403:In Situ Hybridization; D008221:Lymphoid Tissue / Q000382:microbiology*; D016483:Lymphoma, AIDS-Related / Q000382:microbiology*; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction