Pancreastatin increases cytosolic Ca2+ in insulin secreting RINm5F cells.
We have investigated the effect of pancreastatin on cytosolic Ca2+ concentration in the insulin secreting cell line RINm5F. Changes in [Ca2+]i induced by pancreastatin were detected by Fluo-3 fluorescence using both flow cytometry and batch analysis measurements, and turned out to be from 90 to 315 nM equivalent to 80% of that caused by ATP, which increased [Ca2+]i from 90 nM to 400 nM. This effect of pancreastatin did not depend on extracellular calcium and was not mediated by alpha-adrenergic receptors since it was not prevented by the alpha-blocker yohimbine. It is concluded that pancreastatin has a role in the homeostasis of free cytosolic calcium in the insulin secreting cell line Rinm5F.
Sánchez-Margalet
V
V
Department of Medical Biochemistry and Molecular Biology, Medical School of Sevilla, Hospital Virgen Macarena, University of Sevilla, Spain.
Lucas
M
M
Goberna
R
R
D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D002460:Cell Line; D053379:Chromogranin A; D003000:Clonidine / Q000494:pharmacology; D003600:Cytosol / Q000378:metabolism*; D005434:Flow Cytometry; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D010187:Pancreatic Hormones / Q000494:pharmacology*; D011942:Receptors, Adrenergic, alpha / Q000187:drug effects / Q000502:physiology; D015016:Yohimbine / Q000494:pharmacology
D053379:Chromogranin A; D007328:Insulin; D010187:Pancreatic Hormones; D011942:Receptors, Adrenergic, alpha; C050836:pancreastatin; D015016:Yohimbine; D000001:Calcimycin; D000255:Adenosine Triphosphate; D003000:Clonidine; D002118:Calcium