title: In vitro infection of normal human keratinocytes by human papillomavirus type 1 followed by amplification of the viral genome in reconstructed epidermis.
abstract: Primary cultures of normal human keratinocytes were inoculated in vitro with human papillomavirus type 1 (HPV-1), the agent responsible for deep plantar warts. Upon transfer to dead de-epidermized dermis and growth at the air-liquid interface, keratinocytes reconstituted a pseudoepidermis. Under these highly differentiating conditions, HPV-1 DNA amplification was found to take place in the reconstructed epidermis, being detectable from 7 days after the transfer and persisting for at least 10 days thereafter. The extent of keratinocyte differentiation may be insufficient to allow a complete HPV infectious cycle.
authors.affiliation: Department of Molecular Biology, Université Libre de Bruxelles, Rhode Saint Genèse, Belgium.
authors.forename: M
authors.initials: M
authors.lastname: Bossens
authors.forename: C
authors.initials: C
authors.lastname: van Pachterbeke
authors.forename: M
authors.initials: M
authors.lastname: Tuynder
authors.forename: D
authors.initials: D
authors.lastname: Parent
authors.forename: M
authors.initials: M
authors.lastname: Heenen
authors.forename: J
authors.initials: J
authors.lastname: Rommelaere
mesh_terms: D002454:Cell Differentiation; D002478:Cells, Cultured; D004279:DNA, Viral / Q000032:analysis; D004848:Epithelium / Q000382:microbiology; D006801:Humans; D066298:In Vitro Techniques; D015603:Keratinocytes / Q000382:microbiology*; D008297:Male; D027383:Papillomaviridae / Q000254:growth & development*; D014412:Tumor Virus Infections / Q000382:microbiology*; D014779:Virus Replication
chemical_list: D004279:DNA, Viral