Synergistic stimulation of nitric oxide hemoglobin production in rats by recombinant interleukin 1 and tumor necrosis factor.
Nitric oxide (NO) is formed from arginine in Escherichia coli lipopolysaccharide (LPS) treated rat; however, none of specific cytokine inducing NO generation is yet determined. We studied the effect of interleukin 1 (IL-1) and tumor necrosis factor (TNF) on NO production in rats by detecting NO-hemoglobin in their blood, using electron spin resonance. Either IL-1 or TNF alone stimulated NO-hemoglobin formation. Combined administration of IL-1 and TNF markedly enhanced NO-hemoglobin generation, demonstrating the synergistic character of both stimuli on NO production. Further, LPS and TNF in combination were more potent stimulator of NO-hemoglobin production in rats than each alone.
Kosaka
H
H
Department of Physiology, Medical School, Osaka University, Japan.
Harada
N
N
Watanabe
M
M
Yoshihara
H
H
Katsuki
Y
Y
Shiga
T
T
D000818:Animals; D004357:Drug Synergism; D004578:Electron Spin Resonance Spectroscopy; D006442:Glycated Hemoglobin / Q000378:metabolism*; D007375:Interleukin-1 / Q000494:pharmacology*; D007700:Kinetics; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D009569:Nitric Oxide / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D011994:Recombinant Proteins / Q000494:pharmacology; D012016:Reference Values; D014409:Tumor Necrosis Factor-alpha
D006442:Glycated Hemoglobin; D007375:Interleukin-1; D008070:Lipopolysaccharides; D009569:Nitric Oxide; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; C058377:hemoglobin A, glycosylated-nitric oxide complex