title: In vitro glycated low-density lipoprotein interaction with human monocyte-derived macrophages.
abstract: Human low-density lipoprotein (LDL) was glycated in vitro (5 days, glucose 50 mmol/l), labelled with 125I, and its binding and uptake by human monocyte-derived macrophages studied. Glycation produced lower binding and lower uptake. Competition experiments using unlabelled LDL (control, glycated, and acetyl-LDL) showed that most glycated LDL was taken up by the apolipoprotein-B100: E receptor pathway. Results suggest that less of the glycated LDL may enter the cells via scavenger receptors, and very minute amount via non-saturable receptor-independent pathways.
authors.affiliation: Biochemistry Laboratory, Faculté de Pharmacie, Université Louis Pasteur, Illkirch, France.
authors.forename: A
authors.initials: A
authors.lastname: Gugliucci Creriche
authors.forename: S
authors.initials: S
authors.lastname: Dumont
authors.forename: J C
authors.initials: JC
authors.lastname: Siffert
authors.forename: A J
authors.initials: AJ
authors.lastname: Stahl
mesh_terms: D001667:Binding, Competitive; D001693:Biological Transport, Active; D017127:Glycation End Products, Advanced; D006031:Glycosylation; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D008077:Lipoproteins, LDL / Q000378:metabolism*; D008264:Macrophages / Q000378:metabolism*; D009000:Monocytes / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000378:metabolism; D018110:Receptors, Lipoprotein; D000093844:Glycated Proteins
chemical_list: D017127:Glycation End Products, Advanced; D008077:Lipoproteins, LDL; D011956:Receptors, Cell Surface; D018110:Receptors, Lipoprotein; C032629:acetyl-LDL; C032531:apolipoprotein B,E receptor; C031797:glycated lipoproteins, LDL; D000093844:Glycated Proteins