Increased ability of murine bone marrow stromal cells immortalized with simian virus 40 (SV40) to retain haemopoietic cells and support long-term haemopoiesis in vitro.
A simple method for short-term recharge of SV40-immortalized marrow stromal cell (MSC) lines based on their specific interaction with the appropriate haemopoietic cells is described. During the first week after the recharge the production of polymorphonuclear and mononuclear blood cells is an order of magnitude higher than in the recharged primary MSC cultures. Progenitor-derived granulopoiesis is predominant. The long-term repopulating potential of the haemopoietic stem cells (HSC) in the recharged cultures is demonstrated when they are cultured in tissue culture wells (Sutherland et al., 1990). The blood cells produced in the later passages show signs of malignancy.
Milchev
G I
GI
Institute of Cell Biology and Morphology, Bulgarian Academy of Sciences, Sofia.
Pavlova
B
B
Roeva
I
I
D000818:Animals; D001853:Bone Marrow / Q000382:microbiology / Q000502:physiology*; D002478:Cells, Cultured; D006410:Hematopoiesis / Q000502:physiology*; D006412:Hematopoietic Stem Cells / Q000502:physiology*; D007958:Leukocyte Count; D007963:Leukocytes, Mononuclear / Q000502:physiology; D008137:Longitudinal Studies; D051379:Mice; D008807:Mice, Inbred BALB C; D009504:Neutrophils / Q000502:physiology; D013539:Simian virus 40 / Q000254:growth & development*