Zinc hydroxide stimulates superoxide production by rat alveolar macrophages.
The effect of zinc hydroxide on superoxide (O2-) production by rat alveolar macrophages was determined by chemiluminescence and by cytochrome c reduction. Zinc ions had no effect on the chemiluminescence of unstimulated alveolar macrophages. By contrast, zinc hydroxide (ZnOH2), a neutralized form of zinc ions, increased the chemiluminescence level and O2- release. Increased O2- release was inhibited by pertussis toxin, isoquinoline sulfonamide and pretreatment with EGTA. These findings indicate that zinc hydroxide formation from zinc compounds can stimulate the O2- production by alveolar macrophages by receptor-mediated and Ca(2+)-dependent process.
Ogino
K
K
Department of Public Health, Yamaguchi University School of Medicine, Ube, Japan.
Izumi
Y
Y
Ishiyama
H
H
Murata
T
T
Kobayashi
H
H
Houbara
T
T
D000818:Animals; D002374:Catalase / Q000494:pharmacology; D003574:Cytochrome c Group / Q000378:metabolism; D006878:Hydroxides / Q000494:pharmacology*; D066298:In Vitro Techniques; D007700:Kinetics; D008163:Luminescent Measurements; D016676:Macrophages, Alveolar / Q000187:drug effects / Q000378:metabolism*; D010084:Oxidation-Reduction; D051381:Rats; D011919:Rats, Inbred Strains; D013431:Sulfates / Q000494:pharmacology; D013482:Superoxide Dismutase / Q000494:pharmacology; D013481:Superoxides / Q000378:metabolism*; D015032:Zinc / Q000494:pharmacology*; D017967:Zinc Compounds; D019287:Zinc Sulfate
D003574:Cytochrome c Group; D006878:Hydroxides; D013431:Sulfates; D017967:Zinc Compounds; D013481:Superoxides; D019287:Zinc Sulfate; D002374:Catalase; D013482:Superoxide Dismutase; D015032:Zinc; C052745:zinc hydroxide