Evidence for two promoters upstream of the pts operon: regulation by the cAMP receptor protein regulatory complex.
Several potential target sites for multiple regulatory mechanisms were previously identified in the 5' flanking region of the pts operon. We have investigated the in vitro interactions of the cAMP receptor protein (CRP).cAMP regulatory complex with two DNA binding sites, by gel mobility-shift assays, and report the analysis of the functional role of each of the binding sites in vivo. Promoter-reporter gene fusion studies identified two CRP.cAMP-dependent promoters (the previously identified P1 and another promoter, P0) upstream of ptsH. The crr promoters (P2) within ptsI may be negatively regulated by CRP.cAMP.
Fox
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DK
McCollum-Pratt Institute, Johns Hopkins University, Baltimore, MD 21218.
Presper
K A
KA
Adhya
S
S
Roseman
S
S
Garges
S
S
D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics / Q000378:metabolism; D003001:Cloning, Molecular; D002373:Cyclic AMP Receptor Protein / Q000378:metabolism*; D004269:DNA, Bacterial / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D015971:Gene Expression Regulation, Enzymologic; D005798:Genes, Bacterial; D009876:Operon; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000235:genetics*; D011401:Promoter Regions, Genetic; D015183:Restriction Mapping; D014158:Transcription, Genetic
D002373:Cyclic AMP Receptor Protein; D004269:DNA, Bacterial; D015500:Chloramphenicol O-Acetyltransferase; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System