Phorbol ester alters carbachol-stimulated diacylglycerol formation in parotid acinar cells through the hydrolysis of phosphoinositides and phosphatidylcholine.
Incubation of rat parotid acinar cells with phorbol 12,13-dibutyrate (PDBu) resulted in inhibition of carbachol-stimulated formation of sn-1,2-diacylglycerol (DAG). PDBu pretreatment inhibited carbachol-induced turnover of phosphoinositides; this inhibition was indicated by phosphatidylinositol 4,5-bisphosphate breakdown. This pretreatment also attenuated the effect of carbachol on inositol phosphate generation and phosphatidylcholine hydrolysis. These results show that PDBu alters carbachol-stimulated DAG formation through the hydrolysis of phosphoinositides and phosphatidylcholine.
Komabayashi
T
T
Department of Physiology and Pharmacology, Tokyo College of Pharmacy, Japan.
Noguchi
M
M
Izawa
T
T
Suda
K
K
Sawada
H
H
Tsuboi
M
M
D000818:Animals; D002217:Carbachol / Q000494:pharmacology*; D004075:Diglycerides / Q000378:metabolism*; D006868:Hydrolysis; D008297:Male; D010306:Parotid Gland / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D015240:Phorbol 12,13-Dibutyrate / Q000494:pharmacology*; D010713:Phosphatidylcholines / Q000378:metabolism; D010716:Phosphatidylinositols / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
D004075:Diglycerides; D010713:Phosphatidylcholines; D010716:Phosphatidylinositols; D015240:Phorbol 12,13-Dibutyrate; D002217:Carbachol