Detection of antibodies to ovine lentivirus using a recombinant antigen derived from the env gene.
The Western blot assay was performed to characterize antibodies to the transmembrane glycoprotein (TGP) of ovine progressive pneumonia virus (OPPV) by using glutathione-S-transferase-TGP (GST-TGP) protein. The GST-TGP protein was efficiently expressed in Escherichia coli (E. coli) and was highly immunoreactive in the Western blot assay. This assay detected antibodies in 97% (103/106) of the sera from agarose gel immunodiffusion (AGID) positive OPP animals. Like human AIDS patients, antibodies to TGP appear to be one of the major serological markers in OPP infected animals.
Kwang
J
J
USDA, ARS, U.S. Meat Animal Research Center, Clay Center, NE 68933.
Cutlip
R
R
D000595:Amino Acid Sequence; D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology*; D001483:Base Sequence; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D005982:Glutathione Transferase / Q000235:genetics; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology; D012756:Sheep; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology*; D016182:Visna / Q000276:immunology*; D014790:Visna-maedi virus / Q000235:genetics / Q000276:immunology*
D000914:Antibodies, Viral; D011993:Recombinant Fusion Proteins; D014759:Viral Envelope Proteins; D005982:Glutathione Transferase