title: Molecular properties of the vasoactive intestinal peptide receptor in aorta and other tissues.
abstract: The molecular weight of the vasoactive intestinal peptide (VIP) receptor was assessed in bovine aorta, and rat liver, lung, and brain by covalent cross-linking and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The receptor in all four tissues was found to be a single polypeptide of approximate M(r) 54,000, contradicting previous claims for substantial heterogeneity in the molecular weight of this receptor. Guanine nucleotides inhibit cross-linking of 125I-VIP to its receptor, and cross-linking with ethylene glycolbis(succinimidylsuccinate) provides further evidence for complex formation between VIP, its receptor and a guanine nucleotide-binding regulatory protein (G-protein). The precise mechanism of receptor-G-protein coupling may differ between the aorta and other tissues.
authors.affiliation: Department of Pharmacology, College of Medicine, University of Vermont, Burlington 05405.
authors.forename: S M
authors.initials: SM
authors.lastname: Shreeve
authors.forename: A W
authors.initials: AW
authors.lastname: DeLuca
authors.forename: N L
authors.initials: NL
authors.lastname: Diehl
authors.forename: J C
authors.initials: JC
authors.lastname: Kermode
mesh_terms: D000818:Animals; D001011:Aorta / Q000378:metabolism*; D001921:Brain / Q000378:metabolism; D003432:Cross-Linking Reagents; D019204:GTP-Binding Proteins / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008168:Lung / Q000378:metabolism; D008297:Male; D008970:Molecular Weight; D051381:Rats; D017207:Rats, Sprague-Dawley; D011964:Receptors, Gastrointestinal Hormone / Q000737:chemistry* / Q000378:metabolism; D018005:Receptors, Vasoactive Intestinal Peptide; D014018:Tissue Distribution; D014660:Vasoactive Intestinal Peptide / Q000378:metabolism*
chemical_list: D003432:Cross-Linking Reagents; D011964:Receptors, Gastrointestinal Hormone; D018005:Receptors, Vasoactive Intestinal Peptide; D014660:Vasoactive Intestinal Peptide; D019204:GTP-Binding Proteins