title: Immobilization of invertase through its carbohydrate moiety on Ocimum basilicum seed.
abstract: Yeast invertase, a glycoprotein, was covalently coupled to Ocimum basilicum seeds either through its protein or carbohydrate moiety. Of the various methods investigated, binding of the enzyme through its carbohydrate moiety resulted in the retention of considerably higher amounts of enzyme activity. Immobilized invertase showed a shift in the pH optimum toward the alkaline side without appreciable change in temperature optimum. However, the immobilized preparation was more thermostable than the free enzyme. Invertase bound to the seeds could be used repeatedly for the hydrolysis of sucrose syrups in a batch process without appreciable loss in activity. The seeds could serve as an inexpensive, ready-to-use, natural pellicular polysaccharide support for immobilizing enzymes.
authors.affiliation: Food Technology and Enzyme Engineering Division, Bhabha Atomic Research Centre, Bombay, India.
authors.forename: J S
authors.initials: JS
authors.lastname: Melo
authors.forename: S F
authors.initials: SF
authors.lastname: D'Souza
mesh_terms: D002241:Carbohydrates / Q000737:chemistry*; D004795:Enzyme Stability; D004800:Enzymes, Immobilized; D004852:Epoxy Compounds / Q000737:chemistry; D005027:Ethylene Oxide / Q000032:analysis; D005029:Ethylenediamines; D005976:Glutaral; D006026:Glycoside Hydrolases / Q000032:analysis / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D010084:Oxidation-Reduction; D010504:Periodic Acid; D012639:Seeds / Q000737:chemistry*; D013696:Temperature; D043324:beta-Fructofuranosidase
chemical_list: D002241:Carbohydrates; D004800:Enzymes, Immobilized; D004852:Epoxy Compounds; D005029:Ethylenediamines; D010504:Periodic Acid; C031234:ethylenediamine; C009288:metaperiodate; D006026:Glycoside Hydrolases; D043324:beta-Fructofuranosidase; D005027:Ethylene Oxide; D005976:Glutaral