title: Identification of two families of satellite-like repetitive DNA sequences from the zebrafish (Brachydanio rerio).
abstract: To further our understanding of the structure and organization of the zebrafish genome, we have undertaken the analysis of highly and middle-repetitive DNA sequences. We have cloned and sequenced two families of tandemly repeated DNA fragments. The monomer units of the Type I satellite-like sequence are 186 bp long, A+T-rich (65%), and exhibit a high degree of sequence conservation. The Type I satellite-like sequence constitutes 8% of the zebrafish genome, or approximately 8 x 10(5) copies per haploid genome. Southern analysis of genomic DNA, digested with several restriction endonucleases, shows a ladder of hybridizing bands, consistent with a tandem array, and suggests longer range periodic variations in the sequence of the tandem repeats. The Type II satellite has a monomer length of 165 bp, is also A+T-rich (68%), and constitutes 0.2% of the zebrafish genome (22,000 copies per haploid genome). Southern analysis reveals a complex pattern rather than a ladder of regularly spaced hybridizing bands.
authors.affiliation: Institute of Neuroscience, University of Oregon, Eugene 97403.
authors.forename: M
authors.initials: M
authors.lastname: Ekker
authors.forename: A
authors.initials: A
authors.lastname: Fritz
authors.forename: M
authors.initials: M
authors.lastname: Westerfield
mesh_terms: D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004276:DNA, Satellite / Q000235:genetics*; D015698:Genomic Library; D008969:Molecular Sequence Data; D012091:Repetitive Sequences, Nucleic Acid; D015027:Zebrafish / Q000235:genetics*
chemical_list: D004276:DNA, Satellite