Age-related alterations in second-messenger systems in the gerbil brain: autoradiographic analysis and effect of vinconate.
Age-related alterations in binding sites of major second-messenger systems in the gerbil brain were analyzed by receptor autoradiography using [3H]phorbol 12,13-dibutyrate (PDBu), and [3H]inositol 1,4,5-trisphosphate (IP3). In middle-aged gerbils (16 months old), the reduction of PDBu-binding sites was noticed in various brain regions compared with young animals (4 weeks old). By contrast, the elevation of IP3-binding sites was seen in most of brain regions except for the reduction in the cerebellum in the middle-aged animals. Chronic treatment with vinconate partly regulated the age-related changes in the binding sites of these second messengers. The result demonstrates the age-related alterations in the binding sites of an intracellular second-messenger system. Furthermore, they suggest that vinconate partly modulates the age-related alterations in the bindings of second messengers.
Araki
T
T
Department of Neurology, Tohoku University School of Medicine, Japan.
Kanai
Y
Y
Kato
H
H
Kogure
K
K
Ishida
Y
Y
D000375:Aging / Q000502:physiology*; D000818:Animals; D001345:Autoradiography; D001921:Brain / Q000187:drug effects* / Q000378:metabolism*; D029742:Caenorhabditis elegans Proteins; D015220:Calcium Channels; D002352:Carrier Proteins; D005849:Gerbillinae; D015544:Inositol 1,4,5-Trisphosphate / Q000378:metabolism; D053496:Inositol 1,4,5-Trisphosphate Receptors; D007295:Inositol Phosphates / Q000378:metabolism; D008297:Male; D015240:Phorbol 12,13-Dibutyrate / Q000378:metabolism; D011493:Protein Kinase C; D011956:Receptors, Cell Surface / Q000378:metabolism; D018160:Receptors, Cytoplasmic and Nuclear; D011955:Receptors, Drug / Q000378:metabolism; D015290:Second Messenger Systems; D014748:Vinca Alkaloids / Q000494:pharmacology*
D029742:Caenorhabditis elegans Proteins; D015220:Calcium Channels; D002352:Carrier Proteins; D053496:Inositol 1,4,5-Trisphosphate Receptors; D007295:Inositol Phosphates; D011956:Receptors, Cell Surface; D018160:Receptors, Cytoplasmic and Nuclear; D011955:Receptors, Drug; D014748:Vinca Alkaloids; C083141:phorbol ester binding protein; C027776:phorbol ester receptor; D015240:Phorbol 12,13-Dibutyrate; C038627:vinconate; D015544:Inositol 1,4,5-Trisphosphate; D011493:Protein Kinase C