title: Phenotypic properties of 3T3 cells transformed in vitro with polyoma virus and passaged once in syngeneic animals.
abstract: Cloned BALB/c 3T3 cells transformed in vitro with polyoma virus (PyV) acquired a higher tumorigenicity phenotype after a single in vivo passage. Some of the in vivo passaged cells (CTC cells) exhibited also a higher metastatic phenotype than cells from the same clones that were maintained only in culture (C cells). A phenotypic comparison between CTC and C cells was performed. It was found that most CTC lines exhibited a higher binding to laminin compared to their clonal C cell ancestors. Some CTC cells were less sensitive to the cytotoxic effects of TNF-alpha than the corresponding C cells. CTC cells originating from tumors which appeared after a long latency period (late tumors) tended to express Fc gamma RII while CTC cells originating from tumors which appeared after a short latency period (early tumors) as well as the corresponding C cells tended not to express Fc gamma RII. The expression of a membrane epitope recognized by a monoclonal antibody expressing specificity towards PyV transformed cells, was down-regulated on late tumor cells compared to early tumor cells. Transfection of cloned PyV-transformed BALB/c 3T3 cells with the beta 1Fc gamma RII gene augmented the tumorigenicity and metastatic phenotype of the transfectants compared to control transfectants.
authors.affiliation: Department of Cell Research and Immunology, George S. Wise Faculty of Life Sciences, Tel Aviv University, Israel.
authors.forename: A B
authors.initials: AB
authors.lastname: Langer
authors.forename: N
authors.initials: N
authors.lastname: Emmanuel
authors.forename: J
authors.initials: J
authors.lastname: Even
authors.forename: W H
authors.initials: WH
authors.lastname: Fridman
authors.forename: O
authors.initials: O
authors.lastname: Gohar
authors.forename: B
authors.initials: B
authors.lastname: Gonen
authors.forename: B Z
authors.initials: BZ
authors.lastname: Katz
authors.forename: M
authors.initials: M
authors.lastname: Ran
authors.forename: N I
authors.initials: NI
authors.lastname: Smorodinsky
authors.forename: I P
authors.initials: IP
authors.lastname: Witz
mesh_terms: D016475:3T3 Cells / Q000276:immunology* / Q000637:transplantation; D000818:Animals; D002461:Cell Line, Transformed; D002471:Cell Transformation, Neoplastic / Q000276:immunology; D002472:Cell Transformation, Viral; D005260:Female; D016130:Immunophenotyping; D051379:Mice; D008807:Mice, Inbred BALB C; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000209:etiology / Q000276:immunology*; D011120:Polyomavirus / Q000276:immunology*; D014162:Transfection