Immunological identification of a Cl- channel protein in electric organs of Narke japonica.
A Cl- channel in electric organs of Narke japonica was reconfirmed, using a polyclonal antibody, to be a 180k protein composed of two identical 90k units. The specific antibody against the 180k protein from electric organs reduced, when added in the cis side solution, the open probability of the Cl- channel in a planar bilayer membrane without affecting the single channel conductance, ion selectivity nor voltage dependency. Furthermore, the antibody added in the trans side also affected the channel to increase the open probability. The eluate of the immuno-affinity chromatography was found to contain only the 180k protein. These results indicate that 180k protein to be an integral membrane protein and to form the Cl- channel.
Taguchi
T
T
Department of Biophysical Engineering, Faculty of Engineering Science, Osaka University, Japan.
Kawasaki
T
T
Kasai
M
M
D000818:Animals; D018118:Chloride Channels; D002846:Chromatography, Affinity / Q000379:methods; D004553:Electric Conductivity; D004557:Electric Organ / Q000737:chemistry* / Q000276:immunology; D015640:Ion Channel Gating / Q000502:physiology*; D008565:Membrane Proteins / Q000302:isolation & purification* / Q000378:metabolism*; D014101:Torpedo
D018118:Chloride Channels; D008565:Membrane Proteins