Inhibition of mouse hepatitis virus multiplication by an oligonucleotide complementary to the leader RNA.
An oligonucleotide complementary to a leader RNA of positive-stranded mouse hepatitis virus (MHV) was tested for the effect on the viral multiplication in mouse DBT cells. A 14-mer antisense oligonucleotide contained a sequence complementary to the conserved pentanucleotide sequence, UCUAA, of the leader RNA. A treatment of MHV-infected cells with the antisense oligonucleotide at concentrations from 5 to 25 microM had an inhibitory effect on the viral multiplication and reduced the synthesis of viral specific mRNA and proteins. No inhibitory effect was observed when the cells were treated with sense oligonucleotide and oligonucleotide which contained unrelated sequences at concentrations from 1 to 10 microM. These results showed that antisense oligonucleotide against the leader RNA reduced the multiplication of positive-stranded RNA virus, MHV.
Mizutani
T
T
Department of Laboratory Animal Science, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
Hayashi
M
M
Maeda
A
A
Yamashita
T
T
Isogai
H
H
Namioka
S
S
D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002460:Cell Line; D051379:Mice; D008969:Molecular Sequence Data; D006517:Murine hepatitis virus / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development; D016376:Oligonucleotides, Antisense / Q000737:chemistry / Q000494:pharmacology*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics*; D012367:RNA, Viral / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics; D014764:Viral Proteins / Q000096:biosynthesis
D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger; D012367:RNA, Viral; D014764:Viral Proteins