Identification of the glycoprotein H gene of murine cytomegalovirus.
Partial sequencing of the HindIII C fragment of murine cytomegalovirus (MCMV) revealed an open reading frame of 2172 nucleotides in length encoding a 724 amino acid protein with a predicted Mr of 80.4K. Analysis of the predicted amino acid sequence revealed homology with glycoprotein H (gH) from a number of other herpesviruses. MCMV gH showed strongest amino acid identity with human (H) CMV and human herpesvirus 6 gH, and less identity with the gH protein sequences of Epstein-Barr virus, varicella-zoster virus and herpes simplex virus type 1. The greatest identity between MCMV and HCMV gH occurs in the C-terminal region. The MCMV gH is characterized by having a 14 amino acid signal sequence, a 23 amino acid transmembrane region, a seven amino acid positively charged cytoplasmic anchor sequence and eight putative N-linked glycosylation sites. Comparison of MCMV gH with that of HCMV indicates that there are 12 conserved cysteine residues and three conserved potential N-linked glycosylation sites.
Xu
J
J
Department of Microbiology, University of Western Australia, Queen Elizabeth II Medical Centre, Nedlands.
Dallas
P B
PB
Lyons
P A
PA
Shellam
G R
GR
Scalzo
A A
AA
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003587:Cytomegalovirus / Q000235:genetics*; D005814:Genes, Viral; D006023:Glycoproteins / Q000737:chemistry / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000235:genetics*
D006023:Glycoproteins; D014759:Viral Envelope Proteins