[Cytochemical evaluation of blast cells in acute myeloblastic leukemia after induction of their maturation in liquid culture--diagnostic usefulness].
We have performed the cytochemical analysis (myeloperoxidase, ASD-chloroacetate and acid alpha-naphthylacetate esterases, Sudan black B) of blast cells from 25 acute leukemia patients, after 3, 5 and 7 days of liquid culture with conditioned medium from phytohemagglutinin stimulated leukocytes (PHA-LCM). In case of acute myeloid leukemia blast cells an increase of percentage of positive cells simultaneously with the enhancement of the cytochemical reactions was observed. This method may be useful for the precise diagnosis of poorly differentiated blasts with weak expression of cytochemical phenotype.
Hansz
J
J
Kliniki Hematologii Instytutu Chorób Wewnetrznych Akademii Medycznej w Poznaniu.
Sawiński
K
K
Woźny
T
T
D000293:Adolescent; D000328:Adult; D000368:Aged; D001391:Azo Compounds; D001757:Blastomeres / Q000166:cytology; D001853:Bone Marrow / Q000473:pathology*; D002265:Carboxylic Ester Hydrolases / Q000097:blood / Q000378:metabolism*; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D003470:Culture Media; D005260:Female; D006412:Hematopoietic Stem Cells / Q000187:drug effects / Q000201:enzymology / Q000473:pathology*; D006801:Humans; D066298:In Vitro Techniques; D015470:Leukemia, Myeloid, Acute / Q000097:blood / Q000175:diagnosis / Q000201:enzymology / Q000473:pathology*; D007958:Leukocyte Count / Q000187:drug effects; D007962:Leukocytes / Q000187:drug effects / Q000201:enzymology / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D009281:Naphthalenes; D009283:Naphthol AS D Esterase / Q000097:blood / Q000378:metabolism*; D009195:Peroxidase / Q000097:blood / Q000378:metabolism*; D010835:Phytohemagglutinins / Q000494:pharmacology
D001391:Azo Compounds; D003470:Culture Media; D009281:Naphthalenes; D010835:Phytohemagglutinins; C016118:Sudan Black B; D009195:Peroxidase; D009283:Naphthol AS D Esterase; D002265:Carboxylic Ester Hydrolases; C019873:chloroacetate esterase
Ocena cytochemiczna komórek blastycznych ostrych białaczek szpikowych indukowanych do dojrzewania w warunkach hodowli płynnej--przydatność diagnostyczna.