[Lipolysis in model membranes in the presence of positively charged soluble proteins].
Phospholipase A2 hydrolysis of neutral and negatively charged lipid membranes modified by positively charged proteins has been studied using liposomes composed of either dioleoylphosphatidylcholine (DOPC) or dioleoylphosphatidylglycerol (DOPG) alone or their equimolar mixture in the presence of cytochrome c, histone H1, cytochrome b5, and polylysine. Twenty minutes after the reaction had been initiated, DOPC hydrolysis was 58%, while that in the equimolar mixture with DOPG was 35%. DOPG hydrolysis was more complete in binary mixtures of liposomes. The same was observed for liposomes in the presence of cytochrome c. Hydrolysis of phospholipids in binary liposomes in the presence of histone H1 was 3 times faster than that in protein-free liposomes. In the presence of polylysine the rate of DOPG hydrolysis was decreased. The results obtained are suggestive of electrostatic interactions between hydrophilic proteins and negatively charged phospholipids, the phospholipase A2 catalytic activity being affected by these interactions.
Naubatova
M K
MK
Litvinko
N M
NM
Kisel'
M A
MA
Akhrem
A A
AA
D003574:Cytochrome c Group / Q000378:metabolism; D015786:Cytochromes b5 / Q000378:metabolism; D004560:Electricity; D006657:Histones / Q000378:metabolism; D008066:Lipolysis; D008081:Liposomes; D008567:Membranes, Artificial; D010713:Phosphatidylcholines / Q000378:metabolism; D010715:Phosphatidylglycerols / Q000378:metabolism; D010741:Phospholipases A / Q000378:metabolism; D054467:Phospholipases A2; D011107:Polylysine / Q000378:metabolism; D011506:Proteins / Q000378:metabolism*
D003574:Cytochrome c Group; D006657:Histones; D008081:Liposomes; D008567:Membranes, Artificial; D010713:Phosphatidylcholines; D010715:Phosphatidylglycerols; D011506:Proteins; D011107:Polylysine; C051388:1,2-dioleoyl-sn-glycero-3-phosphoglycerol; D015786:Cytochromes b5; D010741:Phospholipases A; D054467:Phospholipases A2; C017251:1,2-oleoylphosphatidylcholine
Issledovanie lipoliza v model'nykh membranakh v prisutstvii polozhitel'no zariazhennykh rastvorimykh belkov.