Direct in vivo gene introduction into rat kidney.
We established a simple and highly efficient method for in vivo gene transfer using HVJ (Sendai virus) and liposomes. Plasmid DNA and high mobility group 1 (HMG1) protein were co-encapsulated in liposomes by agitation and sonication and were co-introduced into cells by HVJ-mediated membrane fusion. pACT SVT DNA, as a reporter gene, was introduced into the kidney of intact rats through a cannula in the renal artery, and SV40 large T antigen was detected by enzyme immunohistochemistry in glomerular cells 4 days after its introduction. This newly developed kidney-directed gene transfer method should be useful not only in basic research but also in potential gene therapeutics of renal diseases.
Tomita
N
N
Department of Geriatric Medicine, Osaka University Medical School, Japan.
Higaki
J
J
Morishita
R
R
Kato
K
K
Mikami
H
H
Kaneda
Y
Y
Ogihara
T
T
D000199:Actins / Q000235:genetics; D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000235:genetics*; D002645:Chickens; D004279:DNA, Viral / Q000008:administration & dosage / Q000235:genetics; D004337:Drug Carriers; D015870:Gene Expression; D005814:Genes, Viral; D006609:High Mobility Group Proteins; D007668:Kidney / Q000502:physiology*; D007678:Kidney Glomerulus / Q000166:cytology / Q000502:physiology; D007700:Kinetics; D008081:Liposomes; D008297:Male; D008561:Membrane Fusion; D010957:Plasmids; D011401:Promoter Regions, Genetic; D051381:Rats; D011919:Rats, Inbred Strains; D013539:Simian virus 40 / Q000235:genetics*; D014162:Transfection
D000199:Actins; D000952:Antigens, Polyomavirus Transforming; D004279:DNA, Viral; D004337:Drug Carriers; D006609:High Mobility Group Proteins; D008081:Liposomes