title: Effect of purified components of Ascaris suum extracts on IgE antibody response in guinea pigs.
abstract: The effect of components P530 and P29, isolated from Ascaris suum adult worm extract (ASC), on the heterologous IgE antibody response was studied in guinea pigs. Groups of 7 guinea pigs were immunized ip with 50 micrograms of ovalbumin (OA) alone or mixed with 200 micrograms of each component precipitated in an alum gel. The primary and secondary IgE antibody response was evaluated by passive cutaneous anaphylaxis reaction (PCA). Immunization of guinea pigs with P29 plus ovalbumin (OA) resulted in a significant increase in the level of serum IgE anti-OA antibodies, especially in the secondary response (almost 8-fold higher when compared with control group). This potentiated response was not observed when the animals received OA plus P530 or the crude extract. Indeed, the P530 component, as well as the crude extract, induced a depression of the anti-OA IgE antibody response (2-3 fold decrease when compared with OA-immunized animals). It was also shown that P29, but not P530 or ASC, was capable of eliciting a strong anti-ASC IgE antibody response. These results demonstrate that in guinea pigs these two Ascaris suum components have antagonistic biological effects, one inducing potentiation and the other suppression of the heterologous IgE antibody response.
authors.affiliation: Centro de Pesquisa e Formação em Imunologia O.G. Bier, Instituto Butantan, São Paulo, Brasil.
authors.forename: M F
authors.initials: MF
authors.lastname: Soares
authors.forename: I
authors.initials: I
authors.lastname: Mota
mesh_terms: D000818:Animals; D000937:Antigen-Antibody Reactions / Q000276:immunology*; D000947:Antigens, Helminth / Q000276:immunology* / Q000302:isolation & purification; D017165:Ascaris suum / Q000276:immunology*; D006168:Guinea Pigs; D007114:Immunization / Q000379:methods; D007073:Immunoglobulin E / Q000032:analysis / Q000276:immunology*; D008970:Molecular Weight; D010323:Passive Cutaneous Anaphylaxis / Q000276:immunology; D013997:Time Factors
chemical_list: D000947:Antigens, Helminth; D007073:Immunoglobulin E