Immunomodulatory effect of peripheral benzodiazepine receptor ligands on human mononuclear cells.
Immunomodulatory effect of ligands active at the peripheral benzodiazepine receptor (PBR) was examined in human peripheral blood mononuclear cells (PBMC). Ro5-4864, PK11195 and diazepam suppressed phytohemagglutinin (PHA) and concanavalin A (ConA) induced proliferation of PBMC. All three ligands inhibited interleukin-3-like activity (IL-3-LA) secretion, while the production of interleukin-2 (IL-2) was inhibited by Ro5-4864 and diazepam only. The selective central benzodiazepine ligand clonazepam did not affect the cellular immune functions examined. Our results indicate an in-vitro immuno-suppressive activity of peripheral and mixed, but not central type benzodiazepine ligands.
Bessler
H
H
Hematology Research Laboratory, Hasharon Hospital, Golda Medical Center, Petah Tiqva, Israel.
Weizman
R
R
Gavish
M
M
Notti
I
I
Djaldetti
M
M
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D000431:Ethanol / Q000494:pharmacology; D005445:Flunitrazepam / Q000494:pharmacology; D006801:Humans; D007107:Immune System / Q000187:drug effects; D007376:Interleukin-2 / Q000378:metabolism; D007377:Interleukin-3 / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000187:drug effects*; D008024:Ligands; D010835:Phytohemagglutinins; D011963:Receptors, GABA-A / Q000378:metabolism*
D000276:Adjuvants, Immunologic; D007376:Interleukin-2; D007377:Interleukin-3; D008024:Ligands; D010835:Phytohemagglutinins; D011963:Receptors, GABA-A; D000431:Ethanol; D005445:Flunitrazepam