Fusion properties of cells infected with human parainfluenza virus type 3: receptor requirements for viral spread and virus-mediated membrane fusion.
Cells can be persistently infected with human parainfluenza virus type 3 (HPF3) by using a high multiplicity of infection (MOI) (> or = 5 PFU per cell). The persistently infected cells exhibit no cytopathic effects and do not fuse with each other, yet they readily fuse with uninfected cells. We have previously shown that the failure of the persistently infected cells to fuse with each other is due to the lack of a receptor on these cells for the viral hemagglutinin-neuraminidase glycoprotein, and we have established that both fusion and hemagglutinin-neuraminidase proteins are needed for cell fusion mediated by HPF3. We then postulated that the generation of persistent infection and the failure of cells infected with HPF3 at high MOI to form syncytia are both due to the action of viral neuraminidase in the high-MOI inoculum. In this report, we describe experiments to test this hypothesis and further investigate the receptor requirements for HPF3 infection and cell fusion. A normally cytopathic low-MOI HPF3 infection can be converted into a noncytopathic infection by the addition of exogenous neuraminidase, either in the form of a purified enzyme or as UV-inactivated HPF3 virions. Evidence is presented that the receptor requirements for an HPF3 virus particle to infect a cell are different from those for fusion between cells. By treating infected cells in culture with various doses of neuraminidase, we demonstrate that virus spreads from cell to cell in the complete absence of cell-cell fusion. We compare the outcome of HPF3 infection in the presence of excess neuraminidase with that of another paramyxovirus (simian virus 5) and provide evidence that these two viruses differ in their receptor requirements for mediating fusion.
Moscona
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A
Department of Pediatrics and Cell Biology, Mount Sinai School of Medicine, New York, New York 10029-6574.
Peluso
R W
RW
D002459:Cell Fusion; D002478:Cells, Cultured; D006389:Hemagglutinins, Viral / Q000378:metabolism; D006801:Humans; D008561:Membrane Fusion; D008954:Models, Biological; D019158:N-Acetylneuraminic Acid; D009439:Neuraminidase / Q000378:metabolism; D010224:Parainfluenza Virus 3, Human / Q000254:growth & development* / Q000472:pathogenicity; D011991:Receptors, Virus / Q000378:metabolism*; D015301:Retroviruses, Simian / Q000378:metabolism / Q000472:pathogenicity; D012794:Sialic Acids / Q000378:metabolism; D014760:Viral Fusion Proteins / Q000378:metabolism; D014779:Virus Replication
D006389:Hemagglutinins, Viral; D011991:Receptors, Virus; D012794:Sialic Acids; D014760:Viral Fusion Proteins; D009439:Neuraminidase; D019158:N-Acetylneuraminic Acid