title: Functional evaluation of human neutrophils. Is the bactericidal activity correlated with nitroblue tetrazolium reduction?
abstract: The cytochemical nitroblue tetrazolium (NBT) reduction test continues to be used in clinical laboratories to detect defects in the oxidative metabolism of phagocytes. However, the specificity of the test is controversial, and it is not clear whether NBT reduction really reflects the microbicidal activity of these cells. In the present study, we evaluated the killing of Staphylococcus aureus by neutrophils from healthy adult individuals and from patients with phagocyte dysfunctions using a fluorochrome phagocytic assay, and compared the results with those obtained with a cytochemical NBT test performed simultaneously. The ability of neutrophils to reduce NBT (expressed as percent reducing neutrophils) with or without a lipopolysaccharide stimulus was not correlated with the bactericidal activity of these cells (expressed as percent killed bacteria per 100 neutrophils). The age and sex of the healthy adults did not influence the results of either assay. It seems that the superoxide anion played a small role in NBT reduction by normal neutrophils, since superoxide dismutase did not significantly inhibit this reaction. Only the absolute absence of NBT reduction reflected the low bactericidal activity of neutrophils, as seen in patients with chronic granulomatous disease (CGD). We conclude that the only clinical usefulness of the NBT test is for the screening of CGD, and that bacterial phagocytic assays are more appropriate for assessing the microbicidal function of neutrophils.
authors.affiliation: Seção de Imunologia, Instituto Adolfo Lutz, São Paulo, Brasil.
authors.forename: R
authors.initials: R
authors.lastname: Bellinati-Pires
authors.forename: M M
authors.initials: MM
authors.lastname: Carneiro-Sampaio
authors.forename: G M
authors.initials: GM
authors.lastname: Colletto
mesh_terms: D000293:Adolescent; D000328:Adult; D001770:Blood Bactericidal Activity / Q000276:immunology / Q000502:physiology*; D005260:Female; D006105:Granulomatous Disease, Chronic / Q000097:blood / Q000276:immunology / Q000503:physiopathology*; D006801:Humans; D066298:In Vitro Techniques; D007223:Infant; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000187:drug effects / Q000276:immunology / Q000502:physiology*; D009580:Nitroblue Tetrazolium / Q000378:metabolism*; D010084:Oxidation-Reduction; D010587:Phagocytosis; D013211:Staphylococcus aureus / Q000276:immunology; D013482:Superoxide Dismutase / Q000494:pharmacology
chemical_list: D009580:Nitroblue Tetrazolium; D013482:Superoxide Dismutase