Effect of a partial deletion of Y chromosome on in vitro fertilizing ability of mouse spermatozoa.
The effect of a partial deletion of Y chromosome on sperm fertilizing ability was investigated through an in vitro fertilization technique. Epididymal spermatozoa of a congenic line, B10.BR-Ydel, which is characterized by a high incidence of abnormal spermatozoa, revealed a significantly lower in vitro fertilization rate (22%) than that (79%) of its control strain (B10.BR/SgSn), which has a normal-sized Y chromosome. Incidence of capacitated spermatozoa as determined by chlortetracycline fluorescence assay was significantly lower in B10.BR-Ydel than in B10.BR/SgSn spermatozoa. The fertilization rate was significantly improved when B10.BR-Ydel spermatozoa were separated from the supernatant of sperm suspension by Percoll gradient centrifugation. A reconstitution experiment revealed that the B10.BR-Ydel spermatozoa were more sensitive to the inhibitory effect of the supernatant than B10.BR/SgSn spermatozoa. Spermatozoa from F1 (C57BL/6N male x B10.BR-Ydel female) males showed higher fertilization rates than those from F1 (B10.BR.Ydel male x C57BL/6N female) males. These observations suggest that not only the morphology but also the fertilizing ability of spermatozoa is directly related to partial deletion of Y chromosome.
Xian
M
M
Department of Animal Pathology, University of Tokyo, Japan.
Azuma
S
S
Naito
K
K
Kunieda
T
T
Moriwaki
K
K
Toyoda
Y
Y
D000818:Animals; D002469:Cell Separation; D002872:Chromosome Deletion; D005260:Female; D005307:Fertilization in Vitro; D006824:Hybridization, Genetic; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D011205:Povidone; D012822:Silicon Dioxide; D013075:Sperm Capacitation; D013094:Spermatozoa / Q000502:physiology*; D014998:Y Chromosome
C016039:Percoll; D012822:Silicon Dioxide; D011205:Povidone