Structural and functional correlates of a mutation in the malignant hyperthermia-susceptible pig ryanodine receptor.
The skeletal muscle ryanodine receptor of malignant hyperthermia-susceptible (MHS) pigs contains a mutation at residue 615 that is highly correlated with various abnormalities in the regulation of sarcoplasmic reticulum (SR) Ca2+ channel activity. In isolated SR membranes the Arg615 to Cys615 ryanodine receptor mutation is now shown to be directly responsible for an altered tryptic peptide map, due to the elimination of the Arg615 cleavage site. Furthermore, trypsin treatment released 86-99 kDa ryanodine receptor fragments encompassing residue 615 from the SR membranes. We conclude that the 86-99 kDa domain containing residue 615 is near the cytoplasmic surface of the ryanodine receptor and likely near important Ca2+ channel regulatory sites.
Mickelson
J R
JR
Department of Veterinary PathoBiology, University of Minnesota, St. Paul 55108.
Knudson
C M
CM
Kennedy
C F
CF
Yang
D I
DI
Litterer
L A
LA
Rempel
W E
WE
Campbell
K P
KP
Louis
C F
CF
D000595:Amino Acid Sequence; D000818:Animals; D001120:Arginine; D001483:Base Sequence; D015220:Calcium Channels / Q000235:genetics*; D003545:Cysteine; D004195:Disease Models, Animal; D008305:Malignant Hyperthermia / Q000235:genetics* / Q000662:veterinary*; D008969:Molecular Sequence Data; D009154:Mutation; D010449:Peptide Mapping; D011950:Receptors, Cholinergic / Q000235:genetics*; D019837:Ryanodine Receptor Calcium Release Channel; D012519:Sarcoplasmic Reticulum / Q000737:chemistry / Q000378:metabolism; D013329:Structure-Activity Relationship; D013552:Swine
D015220:Calcium Channels; D011950:Receptors, Cholinergic; D019837:Ryanodine Receptor Calcium Release Channel; D001120:Arginine; D003545:Cysteine