Changes in cell attachment by RSV transformation in rat liver epithelial cells.
BRL, a non-malignant rat liver epithelial-like cell line, possessed the ability to adhere through fibronectin to a solid substrate. Oncogenical transformation of these BRL cells with RSV induced a significant decrease in the fibronectin molecules in the extracellular matrix and reduction in its ability to adhere to fibronectin. The alpha 5 and beta 1 subunits of integrin (fibronectin receptor) were quantitatively diminished during RSV transformation in BRL cells. These results suggest that adhesive reduction of BRL cells to a substrate by RSV transformation may be caused by a decrease in cell surface fibronectin and fibronectin receptor molecules.
Mashima
K
K
Laboratory of Biochemistry, College of Science, Rikkyo (St. Paul's) University, Tokyo, Japan.
Mizuguchi
J
J
D000818:Animals; D000906:Antibodies; D001358:Avian Sarcoma Viruses / Q000235:genetics*; D002448:Cell Adhesion; D002460:Cell Line; D002462:Cell Membrane / Q000502:physiology; D002471:Cell Transformation, Neoplastic; D004591:Electrophoresis, Polyacrylamide Gel; D004848:Epithelium / Q000502:physiology; D005109:Extracellular Matrix / Q000502:physiology; D005353:Fibronectins / Q000302:isolation & purification / Q000378:metabolism; D007700:Kinetics; D008099:Liver / Q000502:physiology*; D051381:Rats; D017469:Receptors, Fibronectin / Q000302:isolation & purification / Q000378:metabolism
D000906:Antibodies; D005353:Fibronectins; D017469:Receptors, Fibronectin