The effect of exercise and in vivo treatment with ACTH and norepinephrine on the lipolytic responsiveness of guinea pig (Cavia porcellus) adipose tissue.
1. In adipose slices of the guinea pig (Cavia porcellus), adrenocorticotrophic hormone (ACTH) produced a significantly higher lipolytic response (increased Vmax and decreased Km) as compared to norepinephrine (NE), thus indicating a marked difference with human adipose tissue. 2. Exercise-training caused a significant increase in the affinity of the lipolytic response in adipose tissue towards both ACTH and NE, but had no significant effect on Vmax. 3. Prolonged in vivo treatment with both ACTH and NE significantly decreased the responsiveness (affinity and capacity) of adipose slices towards ACTH. Responsiveness towards NE was much less affected.
Van den Bergh
R
R
Department of Biochemistry, University of Port Elizabeth, Republic of South Africa.
Oelofsen
W
W
Naudé
R J
RJ
Terblanche
S E
SE
D000273:Adipose Tissue / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology; D000324:Adrenocorticotropic Hormone / Q000008:administration & dosage / Q000502:physiology*; D000818:Animals; D006168:Guinea Pigs; D066298:In Vitro Techniques; D007700:Kinetics; D008066:Lipolysis / Q000187:drug effects / Q000502:physiology*; D008297:Male; D009638:Norepinephrine / Q000008:administration & dosage / Q000502:physiology*; D010805:Physical Conditioning, Animal
D000324:Adrenocorticotropic Hormone; D009638:Norepinephrine