[Detection of cytotoxicity of biocompatible materials in MRC-5, HeLa, and RC-IAL cell lines].
The sensitivity of diploid and heteroploid cell lines for detection of cytotoxicity using the agar diffusion method on cell culture, was tested with ascorbic acid solution of different concentrations. A total of 562 samples of 21 various materials were tested. The heteroploid cell line, RC-IAL, showed in relation to the MRC-5 and HeLa cell lines, greater sensitivity because it showed the presence of cytotoxic effect with the lowest concentration used (10 and 25 micrograms/ml) of ascorbic acid and showed greater diameter of cytotoxic halo in 15 samples and equal diameter in 16 of the 43 positive samples (7.6%). Out of 43 positive samples, the MRC-5 line did not show cytotoxicity in 3 sponge samples and 1 of acrylic resin. The PVC (polyvinylchloride) and polyethylene rarely showed positivity, while, the plastic, cotton and acrylic resin demonstrated cytotoxicity in about 5% of samples. We thus suggest the use of the RC-IAL and HeLa cell lines for continuation of this type of analysis at Adolfo Lutz Institute.
Cruz
A S
AS
Instituto Adolfo Lutz, Seção de Culturas Celulares, São Paulo, Brasil.
Figueiredo
C A
CA
Martinez
C H
CH
Gomes
L F
LF
D000818:Animals; D001672:Biocompatible Materials / Q000633:toxicity*; D002460:Cell Line / Q000187:drug effects; D005333:Fetus; D006367:HeLa Cells; D006801:Humans; D007668:Kidney; D008168:Lung; D011817:Rabbits; D012680:Sensitivity and Specificity
D001672:Biocompatible Materials
Detecção da citotoxicidade de materiais biocompatíveis nas linhagens celulares MRC-5, HeLa e RC-IAL.