[Isolation of HAV from sea clam].
Three strains of HAV were isolated directly from clams (Arca Subcrensta Lisckke) collected from sea with cell culture and identified by electron microscopy, immunofluorescent test, ELISA, HAV-cDNA hybridization and neutralization test. The strains were stably passed in 2BS cells with the titer of 4.0-5.0 TCID50/ml. Results indicated that the clams were contaminated by HAV and may cause an outbreak of hepatitis A.
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Shandong Provincial Hygiene and Epidemic Prevention Station.
D000818:Animals; D049872:Bivalvia / Q000382:microbiology*; D006507:Hepatovirus / Q000302:isolation & purification*