Translation inhibition by phosphorothioate oligodeoxynucleotides in cell-free systems.
A detailed comparison was made of the concentration dependence of translation inhibition by phosphorothioate and phosphodiester oligodeoxynucleotides of the same anti-beta-globin sequence in cell-free systems using beta-globin mRNA and unrelated mRNAs as controls. The results confirm that at low concentrations the phosphorothioate oligomer is more potent as an antisense compound, while at higher concentration (greater than 4 microM) it exhibits more nonspecific inhibition than the phosphodiester oligomer for RNase H-mediated translation inhibition.
Ghosh
M K
MK
Pharmacology Department, Georgetown University Medical Center, Washington DC.
Ghosh
K
K
Cohen
J S
JS
D001483:Base Sequence; D002474:Cell-Free System; D004926:Escherichia coli / Q000235:genetics; D005914:Globins / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D016376:Oligonucleotides, Antisense / Q000494:pharmacology*; D014176:Protein Biosynthesis / Q000187:drug effects*; D012333:RNA, Messenger / Q000378:metabolism*; D016914:Ribonuclease H / Q000378:metabolism; D013873:Thionucleotides / Q000494:pharmacology*
D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger; D013873:Thionucleotides; D005914:Globins; D016914:Ribonuclease H