Platinum-diaminobenzidine reaction and its contribution to the quantitation of cytochrome oxidase activity.
The platinum-diaminobenzidine (Pt-DAB) reaction, which yields a black, electron-dense, insoluble, Pt-containing reaction product at the active site of cytochrome oxidase without postosmification, has been developed. This reaction permits us to quantitate the oxidase activity in an individual mitochondrion or in its constitutent part by the energy dispersive X-ray analysis (EDAX), based on the platinum atoms incorporated in the product.
Hiraoka
T
T
Shiga University of Medical Science, Ohtsu City, Japan.
Hirai
K
K
D015100:3,3'-Diaminobenzidine; D000818:Animals; D004577:Electron Probe Microanalysis; D003576:Electron Transport Complex IV / Q000378:metabolism*; D051379:Mice; D008813:Mice, Inbred ICR; D008854:Microscopy, Electron / Q000379:methods; D008930:Mitochondria, Liver / Q000201:enzymology / Q000648:ultrastructure; D010984:Platinum
D015100:3,3'-Diaminobenzidine; D010984:Platinum; D003576:Electron Transport Complex IV