[The identification of alphaviruses by using cDNA probes complementary to the 3'-terminal of the viral genome].
Venezuelan equine encephalomyelitis, Eastern equine encephalomyelitis and Sindbis viruses, as well as members of various serological groups of alphaviruses were identified using synthesized molecular probes complementary to 3'-terminus of the molecule of the appropriate genomic RNA: 32P-single-stranded kDNA and E. coli-cloned 32P-double-stranded kDNA. A high species-specificity of kDNA-probes was demonstrated. The sensitivity of the method was 1 ng of viral RNA overlayed on the nitrocellulose filter.
Vinskaia
A I
AI
Proskriakova
O F
OF
Gorban'
S G
SG
Strelets
I V
IV
Kuznetsova
G I
GI
D000524:Alphavirus / Q000235:genetics / Q000302:isolation & purification*; D004247:DNA / Q000235:genetics*; D015342:DNA Probes; D004279:DNA, Viral / Q000235:genetics*; D004663:Encephalitis Virus, Eastern Equine / Q000235:genetics / Q000302:isolation & purification; D004666:Encephalitis Virus, Venezuelan Equine / Q000235:genetics / Q000302:isolation & purification; D005816:Genetic Complementation Test; D016679:Genome, Viral; D009693:Nucleic Acid Hybridization; D012367:RNA, Viral / Q000235:genetics; D012845:Sindbis Virus / Q000235:genetics / Q000302:isolation & purification; D014158:Transcription, Genetic
D015342:DNA Probes; D004279:DNA, Viral; D012367:RNA, Viral; D004247:DNA
Identifikatsiia al'favirusov s pomoshch'iu kDNK-zondov, komplementarnykh 3'-kontsu virusnogo genoma.