Thapsigargin blocks the induction of long-term potentiation in rat hippocampal slices.
Experiments were performed to investigate whether intact intracellular Ca2+ pools are necessary for long-term potentiation (LTP) in the CA1 region of rat hippocampal slices. Thapsigargin (1 microM), which depletes most intracellular Ca2+ pools by blocking ATP-dependent Ca2+ uptake into intracellular compartments, blocked the induction but not the expression of LTP. Thapsigargin had no effect on synaptic transmission or on responses mediated by N-methyl-D-aspartate (NMDA) receptor activation. These data suggest that Ca2+ release from intracellular stores is required for the induction of LTP.
Harvey
J
J
Department of Pharmacology, Medical School, University of Birmingham, UK.
Collingridge
G L
GL
D000818:Animals; D002118:Calcium / Q000502:physiology; D004558:Electric Stimulation; D005071:Evoked Potentials / Q000187:drug effects; D005260:Female; D006624:Hippocampus / Q000187:drug effects* / Q000502:physiology; D066298:In Vitro Techniques; D051381:Rats; D016194:Receptors, N-Methyl-D-Aspartate / Q000187:drug effects; D009435:Synaptic Transmission / Q000187:drug effects; D013729:Terpenes / Q000494:pharmacology*; D019284:Thapsigargin
D016194:Receptors, N-Methyl-D-Aspartate; D013729:Terpenes; D019284:Thapsigargin; D002118:Calcium