Digoxigenin-labeled nucleic acid probe for the detection of infectious bursal disease virus in infected cells.
A cDNA probe was synthesized from the VP-4 region of a virulent field isolate of infectious bursal disease virus (IBDV). The probe was labeled during synthesis with a non-radioactive steroid hapten, digoxigenin. The probe was used to develop a hybridization assay to detect the presence of IBDV in infected cell-culture and tissue suspensions from the bursa of Fabricius of infected chickens. The test was rapid, reproducible, and sensitive, and it could detect four serologic subtypes of IBDV, including the GLS-5 isolate.
Hathcock
T L
TL
Department of Pathobiology, College of Veterinary Medicine, Auburn University, Alabama 36849-5519.
Giambrone
J J
JJ
D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D002645:Chickens; D015342:DNA Probes; D004076:Digoxigenin; D005347:Fibroblasts / Q000382:microbiology; D007243:Infectious bursal disease virus / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000737:chemistry; D011201:Poultry Diseases / Q000175:diagnosis / Q000382:microbiology*; D012367:RNA, Viral / Q000032:analysis; D012088:Reoviridae Infections / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D012680:Sensitivity and Specificity; D013047:Specific Pathogen-Free Organisms; D014774:Virulence
D015342:DNA Probes; D009841:Oligonucleotides; D012367:RNA, Viral; D004076:Digoxigenin