title: Further physicochemical characterization of the monocyte locomotion inhibition factor (MLIF) produced by Entamoeba histolytica.
abstract: Ultrafiltration, gel-sieve chromatography and HPLC were used to purify MLIF. This material was HCl-hydrolyzed and the amino acids analyzed by HPLC-ortho-ophthaldialdehyde. Two hydrophobic non-polar (ile,leu), two uncharged polar (thr,tyr) and two positively charged (basic) (his, arg) amino acids were found in clear excess to their concentration in AMC and thus may intervene in the composition of MLIF.
authors.affiliation: División de Inmunología, Subjefatura de Investigación, CMN Siglo XXI, IMSS, México, DF.
authors.forename: O
authors.initials: O
authors.lastname: Díaz-Guerra
authors.forename: G
authors.initials: G
authors.lastname: Rico
authors.forename: O
authors.initials: O
authors.lastname: Ladrón de Guevara
authors.forename: P
authors.initials: P
authors.lastname: Padilla
authors.forename: L
authors.initials: L
authors.lastname: García
authors.forename: R R
authors.initials: RR
authors.lastname: Kretschmer
mesh_terms: D000596:Amino Acids; D000818:Animals; D002634:Chemotaxis, Leukocyte / Q000187:drug effects*; D002851:Chromatography, High Pressure Liquid; D004748:Entamoeba histolytica / Q000737:chemistry*; D006801:Humans; D009000:Monocytes / Q000187:drug effects*; D015800:Protozoan Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology
chemical_list: D000596:Amino Acids; D015800:Protozoan Proteins