The lux genes in Photobacterium leiognathi are closely linked with genes corresponding in sequence to riboflavin synthesis genes.
Three open reading frames (ORFs) have been found in the region downstream of the luxG gene in the Photobacterium leiognathi lux operon. These genes (ORF I, II, and III) are not only closely linked to the lux operon and transcribed in the same direction but also show the same organization and code for proteins homologous in sequence to the gene products of ribB, ribA, and ribH of Bacillus subtilis, respectively. The Photobacterium leiognathi gene (ORF II) corresponding to ribA was expressed in Escherichia coli in the bacteriophage T7 promoter-RNA polymerase system and a 40 kDa 35S-labeled polypeptide has been detected on SDS-PAGE. Expression of DNA extending from luxBEG to ORF II inserted between a strong promoter and a reporter gene and transferred by conjugation into Vibrio harveyi did not affect the expression of the reporter gene. The results provide evidence that neither promoter nor terminator sites were present in the DNA between the luxG and ORF II indicating that these genes might be part of the lux operon.
Lee
C Y
CY
Department of Biochemistry, McGill University, Montreal, Quebec, Canada.
Meighen
E A
EA
D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D004926:Escherichia coli / Q000235:genetics; D006136:GTP Cyclohydrolase / Q000235:genetics; D005798:Genes, Bacterial; D008164:Luminescent Proteins; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D009876:Operon; D010776:Photobacterium / Q000235:genetics* / Q000378:metabolism; D011401:Promoter Regions, Genetic; D014176:Protein Biosynthesis; D015183:Restriction Mapping; D012256:Riboflavin / Q000096:biosynthesis*; D012258:Riboflavin Synthase / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D013604:T-Phages / Q000235:genetics; D013728:Terminator Regions, Genetic; D014158:Transcription, Genetic
D001426:Bacterial Proteins; D002352:Carrier Proteins; D004269:DNA, Bacterial; D008164:Luminescent Proteins; D046911:Macromolecular Substances; C026648:lumazine protein, Photobacterium; D012258:Riboflavin Synthase; D006136:GTP Cyclohydrolase; D012256:Riboflavin