Replication-defective virus infection of feather buds produces a localized region of beta-galactosidase activity.
We are interested in using retroviral vectors to trace cell lineage and to introduce exogenous genes in chicken skin explant cultures. Here the LZ10 virus carrying the gene encoding beta-galactosidase was introduced to the skin explants by two different means: a) the virus was added to the media or b) the virus was microinjected into regions of the developing feather buds. Infection by microinjection led to localized expression of beta-galactosidase in the developing feather bud, while, surprisingly, infection by adding the virus to the culture media led to localized band of beta-galactosidase expression in the middle of the feather filament. The significance of this finding in skin morphogenesis and as a tool for experimental embryology is discussed.
Widelitz
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RB
Department of Pathology, School of Medicine, University of Southern California, Los Angeles 90033.
Chuong
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CM
D000818:Animals; D001358:Avian Sarcoma Viruses / Q000235:genetics / Q000502:physiology*; D002472:Cell Transformation, Viral; D002642:Chick Embryo; D003673:Defective Viruses / Q000235:genetics / Q000502:physiology*; D009924:Organ Culture Techniques; D012867:Skin / Q000166:cytology / Q000196:embryology* / Q000201:enzymology; D014779:Virus Replication; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism*
D001616:beta-Galactosidase