N-methyl-N'-nitro-N-nitrosoguanidine: the effect on migration and cGMP level of neutrophils.
N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) causes a concentration-dependent increase of the cGMP level in rabbit peritoneal neutrophils. MNNG has a strong chemokinetic effect on neutrophils. In addition the compound also possesses a moderate chemotactic effect. The potentiating effect of MNNG on neutrophil migration is completely inhibited by pertussis toxin. The results support the view that in neutrophils cGMP is involved in chemokinesis and chemotaxis and that the cGMP-mediated potentiation of migration is controlled by a pertussis toxin-sensitive G-protein.
Elferink
J G
JG
Department of Medical Biochemistry, University of Leiden, The Netherlands.
de Koster
B M
BM
D000818:Animals; D002465:Cell Movement / Q000187:drug effects; D006152:Cyclic GMP / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D066298:In Vitro Techniques; D008769:Methylnitronitrosoguanidine / Q000494:pharmacology*; D009504:Neutrophils / Q000187:drug effects*; D037342:Pertussis Toxin; D011817:Rabbits; D010566:Virulence Factors, Bordetella / Q000494:pharmacology
D010566:Virulence Factors, Bordetella; D008769:Methylnitronitrosoguanidine; D037342:Pertussis Toxin; D006152:Cyclic GMP