Three-dimensional reconstruction of single particles negatively stained or in vitreous ice.
The random-conical reconstruction method has been highly successful in three-dimensional imaging of macromolecules under low-dose conditions. This article summarizes the different steps of this technique as applied to molecules prepared with negative staining or vitreous ice, and sketches out the current directions of development. We anticipate that by using new instrumental developments, transfer function correction and computational refinement techniques, a resolution in the range of 7-10 A could ultimately be achieved.
Frank
J
J
Wadsworth Center for Laboratories and Research, New York State Department of Health, Albany 12201-0509.
Radermacher
M
M
D015220:Calcium Channels / Q000648:ultrastructure; D015925:Cryopreservation; D006433:Hemocyanins / Q000648:ultrastructure; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D008854:Microscopy, Electron / Q000379:methods*; D016624:Negative Staining; D012270:Ribosomes / Q000648:ultrastructure
D015220:Calcium Channels; D006433:Hemocyanins