title: Detection of human papillomavirus (HPV) DNA type 6/11 in a conjunctival papilloma by in situ hybridization with biotinylated probes.
abstract: Four cases of conjunctival papilloma in two different patients were examined by in situ hybridization for HPV DNA type 6/11, 16/18 and 31/33/51. Formalin-fixed and paraffin-embedded tissues were hybridized by biotinylated probes. One tumor and one of its recurrences showed nuclear positivity for HPV 6/11 in the superficial cells of the epithelium. The results suggest that HPV type 6/11 may be etiologic agent of conjunctival papillomas. The benign behavior of these neoplasms may be related to the etiologic role of this type of HPV.
authors.affiliation: Istituto di anatomia e istologia patologica, Università di Firenze.
authors.forename: G P
authors.initials: GP
authors.lastname: Mincione
authors.forename: G L
authors.initials: GL
authors.lastname: Taddei
authors.forename: M
authors.initials: M
authors.lastname: Wolovsky
authors.forename: A
authors.initials: A
authors.lastname: Calzolari
authors.forename: F
authors.initials: F
authors.lastname: Mincione
mesh_terms: D000293:Adolescent; D000328:Adult; D001710:Biotin; D003230:Conjunctival Neoplasms / Q000737:chemistry / Q000382:microbiology*; D015344:DNA Probes, HPV; D004273:DNA, Neoplasm / Q000032:analysis*; D004279:DNA, Viral / Q000032:analysis*; D006801:Humans; D017403:In Situ Hybridization; D008297:Male; D009364:Neoplasm Recurrence, Local; D010212:Papilloma / Q000737:chemistry / Q000382:microbiology*; D027383:Papillomaviridae / Q000145:classification / Q000302:isolation & purification* / Q000472:pathogenicity
chemical_list: D015344:DNA Probes, HPV; D004273:DNA, Neoplasm; D004279:DNA, Viral; D001710:Biotin