Photoaffinity labeling of the electroplax sodium channel with a photoreactive mu-conotoxin carrying a radioactive and chromogenic diazirine.
Photoreactive derivatives of mu-conotoxin GIIIA carrying a radioactive and chromogenic diazirine have been prepared as a photoaffinity labeling reagent for muscle-type sodium channels. When the reagent was photolyzed in the presence of the eel sodium channel, radioactivity was specifically incorporated into the channel protein.
Hatanaka
Y
Y
Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.
Nakayama
H
H
Kanaoka
Y
Y
D000345:Affinity Labels; D000595:Amino Acid Sequence; D000818:Animals; D020916:Conotoxins; D004557:Electric Organ / Q000378:metabolism* / Q000502:physiology; D004593:Electrophorus; D008969:Molecular Sequence Data; D010456:Peptides, Cyclic / Q000737:chemistry*; D010777:Photochemistry; D015222:Sodium Channels / Q000378:metabolism*
D000345:Affinity Labels; D020916:Conotoxins; D010456:Peptides, Cyclic; D015222:Sodium Channels; C046101:conotoxin GIII