Replication of porcine cytomegalovirus in the 19-PFT cell line.
Replication of porcine cytomegalovirus was examined in fibroblast- and epithelial-like cell lines of the 19-PFT cell line derived from pig fallopian tube. The virus grew well in the fibroblast-like cell line than the epithelial-like cell line. Cytomegalic cytopathic effects of the virus were clearly observed under the microscope after dispersion of the infected cell culture by trypsin-versene and it was demonstrated that cytomegalic cytopathic effects could be used for infectivity titration. Intranuclear inclusions were formed in the infected cells and herpetic virus particles were observed in the nucleus and cytoplasm when infected cells were observed under the transmission electron microscope. Infected cells formed characteristic red plaque.
Kawamura
H
H
Department of Veterinary Microbiology, College of Agriculture, University of Osaka Prefecture, Japan.
Tajima
T
T
Hironao
T
T
Kajikawa
T
T
Kotani
T
T
D000818:Animals; D002460:Cell Line; D003470:Culture Media; D003587:Cytomegalovirus / Q000502:physiology*; D004847:Epithelial Cells; D004848:Epithelium / Q000382:microbiology; D005187:Fallopian Tubes / Q000166:cytology / Q000382:microbiology; D005260:Female; D005347:Fibroblasts / Q000382:microbiology; D013552:Swine / Q000382:microbiology*; D014779:Virus Replication
D003470:Culture Media