[Effect of age and phenobarbital on liver activity of mixed function oxidase].
Effect of age and phenobarbital on the rat mixed function oxidase activity was studied. Male Wistar rats 0.5-, 1-, 2-, 4-, 8-, 12-, 20- and 28 month-old were used. In this study the levels of cytochrome P-450 and cytochrome b5. NADPH-cytochrome P-450 and NADH-cytochrome b5 reductases activity were examined. Both cytochrome P-450 and NADPH-cytochrome P-450 reductase activity was induced by phenobarbital in all animals. Maximum was observed in 4- and 8-month-old for hemoprotein and 2-month-old rats for reductase activity but minimum in youngest and oldest one respectively. On the contrary, cytochrome b5 and NADH-cytochrome b5 reductase activity was inhibited after phenobarbital injection. The highest depression of cytochrome b5 content was found in youngest, but the enzyme activity in oldest rats.
Plewka
A
A
II Katedry Histologii i Embriologii, Slaskiej Akademii Medycznej, Katowicach.
Kamiński
M
M
D000375:Aging / Q000378:metabolism*; D000818:Animals; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D006899:Mixed Function Oxygenases / Q000187:drug effects / Q000378:metabolism*; D010634:Phenobarbital / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
D006899:Mixed Function Oxygenases; D010634:Phenobarbital
Wpływ wieku i fenobarbitalu na aktywność watrobowego układu oksydaz o funkcji mieszanej u szczurów.