[Uptake of 86Rb by human erythrocytes: modification of the method and applications].
In this study we applied a method generally used for the study of Na+,K(+)-ATPase, as well as other systems of potassium transport, which makes use of a rubidium isotope (86Rb) as analogue of the potassium and is known as uptake of the 86Rb. This method proved to be particularly sensitive and versatile for kinetic studies of this pump system, allowing to assess possible alterations. Its application in the study of sodium and potassium transport in erythrocytes of uremic subjects in extracorporeal dialysis made it possible to reveal certain alterations due both to pump-dependent and pump-independent uptake. In fact, the results show the hypothesis of restoration of Na+,K(+)-pump activity for elimination during dialysis of one or more inhibitor present in the uremic plasma. Furthermore, a reduction in aspecific flows was noted which could be the result of more generalized damage of the membrane.
Albertini
M C
MC
Istituto di Chimica Biologica G. Fornaini, Università di Urbino.
Canestrari
F
F
Galli
F
F
Pascucci
M
M
Galiotta
P
P
D000328:Adult; D000368:Aged; D004910:Erythrocyte Membrane / Q000378:metabolism*; D006801:Humans; D008875:Middle Aged; D010042:Ouabain / Q000494:pharmacology; D006435:Renal Dialysis; D012414:Rubidium Radioisotopes / Q000097:blood / Q000493:pharmacokinetics*; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors / Q000097:blood / Q000187:drug effects; D014511:Uremia / Q000097:blood / Q000628:therapy
D012414:Rubidium Radioisotopes; D010042:Ouabain; D000254:Sodium-Potassium-Exchanging ATPase
Uptake del 86Rb negli eritrociti umani: messa a punto del metodo ed applicazioni.