title: Changes in the lateral diffusion of fluorescent lipid analogues in the surface membrane of adult male Schistosoma mansoni.
abstract: The effect of serotonin on the fluidity of the tegumental membranes of adult male Schistosoma mansoni was assessed by the fluorescence recovery after photobleaching technique. It was demonstrated that the translational diffusion of 5-N'-octadecanoyl aminofluorescein is reduced by a mechanism involving G-protein coupled activation of adenylate cyclase and lowering of intracellular calcium concentration. Furthermore, the lateral diffusion coefficient and the mobile fraction appear to be controlled by calcium and cAMP dependent pathways respectively. No change in the diffusion of the fluorescent phospholipid N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)-phosphatidyl choline was observed, suggesting the two probes used here partition into two different domains that are under independent control. An increase in the amount of protein associating with a membrane cytoskeleton is also demonstrated.
authors.affiliation: Institute of Biochemistry, University of Glasgow, UK.
authors.forename: W A
authors.initials: WA
authors.lastname: Sands
authors.forename: J R
authors.initials: JR
authors.lastname: Kusel
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D000242:Cyclic AMP / Q000378:metabolism; D003599:Cytoskeleton / Q000187:drug effects / Q000378:metabolism; D004058:Diffusion; D005456:Fluorescent Dyes / Q000493:pharmacokinetics; D008297:Male; D008560:Membrane Fluidity / Q000187:drug effects; D012550:Schistosoma mansoni / Q000187:drug effects / Q000378:metabolism*; D012701:Serotonin / Q000494:pharmacology
chemical_list: D005456:Fluorescent Dyes; D012701:Serotonin; D000242:Cyclic AMP; D002118:Calcium