Vaccinia-rotavirus VP7 recombinants protect mice against rotavirus-induced diarrhoea.
Recombinant vaccinia viruses expressing wild type intracellular VP7 (VP7wt) from rotavirus SA11 or VP7sc, a cell surface-anchored variant, boosted antibody titres in SA11-immune mice. Pups born to these mice were protected from diarrhoea following challenge with SA11. In rotavirus-naive mice, two immunizations with recombinant vaccinia virus expressing VP7sc stimulated protective immunity that could be transferred to pups, whereas viruses expressing VP7wt did not stimulate protective immunity. Recombinant vaccinia viruses expressing intracellular or cell surface-anchored VP6, the rotavirus group-reactive antigen from the inner capsid, did not stimulate protective immunity. These experiments demonstrate that a live viral vector expressing cell surface anchored VP7 may represent a strategy for the development of safe, effective vaccines against rotavirus-induced diarrhoea.
Andrew
M E
ME
CSIRO Australian Animal Health Laboratory, Geelong, Vic.
Boyle
D B
DB
Coupar
B E
BE
Reddy
D
D
Bellamy
A R
AR
Both
G W
GW
D000818:Animals; D000831:Animals, Newborn / Q000276:immunology; D000906:Antibodies / Q000032:analysis; D003967:Diarrhea / Q000382:microbiology / Q000517:prevention & control*; D005260:Female; D007112:Immunity, Maternally-Acquired / Q000276:immunology; D051379:Mice; D008808:Mice, Inbred CBA; D008892:Milk / Q000276:immunology; D012401:Rotavirus / Q000276:immunology*; D012400:Rotavirus Infections / Q000517:prevention & control*; D014614:Vaccines, Synthetic / Q000627:therapeutic use*; D014616:Vaccinia virus / Q000276:immunology*; D014765:Viral Vaccines / Q000627:therapeutic use*
D000906:Antibodies; D014614:Vaccines, Synthetic; D014765:Viral Vaccines