title: Immunomodulatory effect of peripheral benzodiazepine receptor ligands on human mononuclear cells.
abstract: Immunomodulatory effect of ligands active at the peripheral benzodiazepine receptor (PBR) was examined in human peripheral blood mononuclear cells (PBMC). Ro5-4864, PK11195 and diazepam suppressed phytohemagglutinin (PHA) and concanavalin A (ConA) induced proliferation of PBMC. All three ligands inhibited interleukin-3-like activity (IL-3-LA) secretion, while the production of interleukin-2 (IL-2) was inhibited by Ro5-4864 and diazepam only. The selective central benzodiazepine ligand clonazepam did not affect the cellular immune functions examined. Our results indicate an in-vitro immuno-suppressive activity of peripheral and mixed, but not central type benzodiazepine ligands.
authors.affiliation: Hematology Research Laboratory, Hasharon Hospital, Golda Medical Center, Petah Tiqva, Israel.
authors.forename: H
authors.initials: H
authors.lastname: Bessler
authors.forename: R
authors.initials: R
authors.lastname: Weizman
authors.forename: M
authors.initials: M
authors.lastname: Gavish
authors.forename: I
authors.initials: I
authors.lastname: Notti
authors.forename: M
authors.initials: M
authors.lastname: Djaldetti
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D000431:Ethanol / Q000494:pharmacology; D005445:Flunitrazepam / Q000494:pharmacology; D006801:Humans; D007107:Immune System / Q000187:drug effects; D007376:Interleukin-2 / Q000378:metabolism; D007377:Interleukin-3 / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000187:drug effects*; D008024:Ligands; D010835:Phytohemagglutinins; D011963:Receptors, GABA-A / Q000378:metabolism*
chemical_list: D000276:Adjuvants, Immunologic; D007376:Interleukin-2; D007377:Interleukin-3; D008024:Ligands; D010835:Phytohemagglutinins; D011963:Receptors, GABA-A; D000431:Ethanol; D005445:Flunitrazepam