The GABAB receptor antagonist, CGP-35348, inhibits paired-pulse disinhibition in the rat dentate gyrus in vivo.
Extracellular field potentials were recorded from the dentate gyrus of adult rats during electrical stimulation of the angular bundle in vivo. Paired pulses produced inhibition of the second population spike (PS2) at 25 ms, and potentiation at inter-stimulus intervals from 50 to 200 ms. The GABAB receptor antagonist, CGP 35348, reduced the amplitude of PS2 at each of these inter-stimulus intervals while producing no effect on the first population spike of the pair (PS1). Under control conditions, the duration of the second population EPSP (pEPSP2) was increased relative to the first at inter-stimulus intervals from 100 to 400 ms, and CGP 35348 reduced these durations to baseline levels. These results demonstrate that GABAB receptors modulate synaptic inhibition in vivo.
Brucato
F H
FH
Department of Medicine, Duke University, Durham, NC 27710.
Morrisett
R A
RA
Wilson
W A
WA
Swartzwelder
H S
HS
D000818:Animals; D004566:Electrodes; D005071:Evoked Potentials / Q000187:drug effects; D005260:Female; D058787:GABA-A Receptor Antagonists; D006624:Hippocampus / Q000187:drug effects / Q000502:physiology*; D009943:Organophosphorus Compounds / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011963:Receptors, GABA-A / Q000187:drug effects*
D058787:GABA-A Receptor Antagonists; D009943:Organophosphorus Compounds; D011963:Receptors, GABA-A; C066430:CGP 35348