title: Minoxidil specifically decreases the expression of lysine hydroxylase in cultured human skin fibroblasts.
abstract: The levels of lysine hydroxylase protein and the levels of the mRNAs for lysine hydroxylase and the alpha- and beta-subunits of proline 4-hydroxylase were measured in cultured human skin fibroblasts treated with 1 mM-minoxidil. The data demonstrate that minoxidil decreases the amount of lysine hydroxylase protein, this being due to a decrease in the level of lysine hydroxylase mRNA. The effect of minoxidil appears to be highly specific, as no changes were observed in the amounts of mRNAs for the alpha- and beta-subunits of proline 4-hydroxylase.
authors.affiliation: Collagen Research Unit, University of Oulu, Finland.
authors.forename: T
authors.initials: T
authors.lastname: Hautala
authors.forename: J
authors.initials: J
authors.lastname: Heikkinen
authors.forename: K I
authors.initials: KI
authors.lastname: Kivirikko
authors.forename: R
authors.initials: R
authors.lastname: Myllylä
mesh_terms: D015152:Blotting, Northern; D015153:Blotting, Western; D002478:Cells, Cultured; D003864:Depression, Chemical; D005347:Fibroblasts / Q000187:drug effects* / Q000201:enzymology / Q000502:physiology; D015870:Gene Expression / Q000187:drug effects*; D006801:Humans; D015151:Immunoblotting; D046911:Macromolecular Substances; D008914:Minoxidil / Q000494:pharmacology*; D009693:Nucleic Acid Hybridization; D008240:Procollagen-Lysine, 2-Oxoglutarate 5-Dioxygenase / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D011393:Procollagen-Proline Dioxygenase / Q000235:genetics / Q000378:metabolism; D014176:Protein Biosynthesis / Q000187:drug effects; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: D046911:Macromolecular Substances; D012333:RNA, Messenger; D008914:Minoxidil; D011393:Procollagen-Proline Dioxygenase; D008240:Procollagen-Lysine, 2-Oxoglutarate 5-Dioxygenase