Basic fibroblast growth factor in cells derived from Dupuytren's contracture: synthesis, presence, and implications for treatment of the disease.
Dupuytren's contracture (DC) is associated with fibroblast and endothelial cell proliferation. We have identified a fibroblast and endothelial cell mitogen, basic fibroblast growth factor (FGF), in cells derived from this tissue and characterized the effects of this growth factor on DC cells. Northern blot analysis of DC cells reveals the presence of basic FGF mRNA species, and the DC cells coexpress multiple forms of basic FGF. Radioreceptor assays establish that the DC cells have high-affinity binding sites for basic FGF and proliferate in response to exogenous recombinant basic FGF. Furthermore, a conjugate between saporin (a ribosome-inactivating protein) and basic FGF, which is cytotoxic to cells possessing the basic FGF receptor, is also cytotoxic to DC cells. The possibility that basic FGF-saporin could be a potential therapeutic agent for prevention of recurrence of the disease after surgery is discussed.
Lappi
D A
DA
Department of Molecular and Cellular Growth Biology, Whittier Institute, La Jolla, CA 92037.
Martineau
D
D
Maher
P A
PA
Florkiewicz
R Z
RZ
Buscaglia
M
M
Gonzalez
A M
AM
Farris
J
J
Hamer
M
M
Fox
R
R
Baird
A
A
D002455:Cell Division; D004305:Dose-Response Relationship, Drug; D004387:Dupuytren Contracture / Q000473:pathology*; D016222:Fibroblast Growth Factor 2 / Q000032:analysis* / Q000096:biosynthesis / Q000187:drug effects / Q000235:genetics; D005347:Fibroblasts / Q000473:pathology; D006801:Humans; D000922:Immunotoxins; D009699:N-Glycosyl Hydrolases; D010940:Plant Proteins / Q000494:pharmacology; D011505:Protein-Tyrosine Kinases / Q000494:pharmacology; D012333:RNA, Messenger; D011956:Receptors, Cell Surface / Q000032:analysis; D017468:Receptors, Fibroblast Growth Factor; D054789:Ribosome Inactivating Proteins, Type 1; D000076985:Saporins
D000922:Immunotoxins; D010940:Plant Proteins; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D017468:Receptors, Fibroblast Growth Factor; D054789:Ribosome Inactivating Proteins, Type 1; D016222:Fibroblast Growth Factor 2; D011505:Protein-Tyrosine Kinases; D009699:N-Glycosyl Hydrolases; D000076985:Saporins