title: Marked increase in cerebrospinal fluid ubiquitin in Creutzfeldt-Jakob disease.
abstract: We have established the radioimmunoassay for ubiquitin in the cerebrospinal fluid (CSF) and measured the ubiquitin concentration in CSF from 4 cases of neuropathologically verified Creutzfeldt-Jakob disease (CJD), 10 cases of multi-infarct dementia (MID), 7 cases of senile dementia of Alzheimer type (SDAT), and 18 controls. The normal values were determined to range from 7.3 to 21.0 ng/ml, 14.3 +/- 1.1 ng/ml in the mean +/- S.E.M. The CSF ubiquitin levels in the cases of MID and SDAT were 16.6 +/- 6.4 ng/ml and 21.3 +/- 6.1 ng/ml, respectively. In the cases of CJD, the CSF ubiquitin was markedly increased at the early and middle stages of the disease (230.6 ng/ml in Case 1, 107.6 ng/ml in Case 2, 212.5 ng/ml in Case 3, and 377.0 ng/ml in Case 4) and these gradually decreased as the disease progressed. The measurement of CSF ubiquitin seems useful to make an early diagnosis of CJD.
authors.affiliation: Third Department of Internal Medicine, Yamagata University School of Medicine, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Manaka
authors.forename: T
authors.initials: T
authors.lastname: Kato
authors.forename: K
authors.initials: K
authors.lastname: Kurita
authors.forename: T
authors.initials: T
authors.lastname: Katagiri
authors.forename: Y
authors.initials: Y
authors.lastname: Shikama
authors.forename: K
authors.initials: K
authors.lastname: Kujirai
authors.forename: T
authors.initials: T
authors.lastname: Kawanami
authors.forename: Y
authors.initials: Y
authors.lastname: Suzuki
authors.forename: K
authors.initials: K
authors.lastname: Nihei
authors.forename: H
authors.initials: H
authors.lastname: Sasaki
mesh_terms: D000368:Aged; D000544:Alzheimer Disease / Q000134:cerebrospinal fluid; D002851:Chromatography, High Pressure Liquid; D007562:Creutzfeldt-Jakob Syndrome / Q000134:cerebrospinal fluid*; D015161:Dementia, Multi-Infarct / Q000134:cerebrospinal fluid; D006801:Humans; D008875:Middle Aged; D011863:Radioimmunoassay; D014452:Ubiquitins / Q000134:cerebrospinal fluid* / Q000276:immunology
chemical_list: D014452:Ubiquitins