Possible involvement of nuclear oncoproteins in regulation of DNA replication.
Polyomavirus DNA replication requires its enhancer which contains an AP-1 site. We have shown that protooncogenes, c-jun and c-fos, whose products form heterodimeric transcription activator, AP-1, strongly stimulate polyomavirus DNA replication through the AP-1 site. The mechanisms by which this enhancer stimulates replication and transcription are different. By replacing the enhancer with the oligonucleotides representing the binding site of a transcription factor of interest, any transcription factor with the known binding sequence can be characterized in this replication assay. When Rel protein was examined, we were able to reveal a new domain in v-Rel protein which can stimulate replication strongly. Whether this domain also coincides with transforming potential of v-Rel protein is currently under investigation.
Asano
M
M
Department of Viral Oncology, Institute for Virus Research, Kyoto University, Japan.
Ishikawa
H
H
Ito
Y
Y
D000818:Animals; D004261:DNA Replication; D004279:DNA, Viral / Q000235:genetics*; D009687:Nuclear Proteins / Q000502:physiology*; D015513:Oncogene Proteins / Q000502:physiology*; D011120:Polyomavirus / Q000235:genetics*; D014157:Transcription Factors / Q000502:physiology*
D004279:DNA, Viral; D009687:Nuclear Proteins; D015513:Oncogene Proteins; D014157:Transcription Factors