A quantitative assay of retrograde transported HSV in the trigeminal ganglion.
The relationship between the dose of Herpes simplex virus type 1 (HSV) inoculated in the cornea and the amount of actively replicating virus recovered from mouse trigeminal ganglion cells 5 d after corneal scratch and inoculation was investigated with a tissue culture plaque assay. A dose response curve of productive viral replication was obtained. The estimated dose of HSV that produces half-maximal recovery of virus within the ganglion was 9.15 x 10(3) plaque forming units per eye, and the maximal amount of HSV recovered was 1.34 x 10(4) pfu per ganglion. This definition of infectivity as a function of dose will be useful for studying the effects of potential inhibitors of the binding, uptake, and transport of HSV by productively or latently infected trigeminal neurons.
Carter
S R
SR
Department of Anatomy and Neuroscience Program, University of California San Francisco 94143-0452.
Pereira
L
L
Paz
P
P
LaVail
J H
JH
D000818:Animals; D003315:Cornea / Q000294:innervation / Q000382:microbiology; D003389:Cranial Nerve Diseases / Q000382:microbiology; D004195:Disease Models, Animal; D005260:Female; D005455:Fluorescent Antibody Technique; D006561:Herpes Simplex / Q000382:microbiology*; D016849:Keratitis, Herpetic / Q000382:microbiology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D018139:Simplexvirus / Q000502:physiology; D012668:Trigeminal Ganglion / Q000382:microbiology*; D014779:Virus Replication