Extracellular ATP induces rapid cell rounding in cultured human Chang liver cells.
Extracellular ATP (7 microM-14 mM) induced monolayer Chang liver cells (ATCC CCL 13) to retract and round up in dose-dependent and time-dependent manners. ATP-induced rounding was concomitant with raised intracellular pH (7.3 to 7.8) and suppressed (a) in sodium-free medium (250 mM sucrose), (b) with the addition of 1 mM quinidine, an inhibitor of Na+/H+ exchanges, and (c) with the addition of 10 nM staurosporine, a potent inhibitor of protein kinase C that is known to activate Na+/H+ exchanges. These findings suggest that ATP-induced cell rounding is due to activation of Na+/H+ antiporters.
Sit
K H
KH
Department of Anatomy, National University of Singapore, Kent Ridge.
Bay
B H
BH
Wong
K P
KP
D000255:Adenosine Triphosphate / Q000494:pharmacology*; D000470:Alkaloids / Q000494:pharmacology; D000595:Amino Acid Sequence; D002352:Carrier Proteins / Q000378:metabolism; D002478:Cells, Cultured; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008099:Liver / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000494:pharmacology; D011802:Quinidine / Q000494:pharmacology; D017923:Sodium-Hydrogen Exchangers; D019311:Staurosporine
D000470:Alkaloids; D002352:Carrier Proteins; D009842:Oligopeptides; D017923:Sodium-Hydrogen Exchangers; C047981:arginyl-glycyl-aspartic acid; D000255:Adenosine Triphosphate; D019311:Staurosporine; D011802:Quinidine