The effect of the UL42 protein on the DNA polymerase activity of the catalytic subunit of the DNA polymerase encoded by herpes simplex virus type 1.
The effect that the UL42 protein of herpes simplex virus type 1 has on the DNA polymerase activity of the DNA polymerase catalytic subunit (Pol) of the same virus has been investigated. The observed effects are critically dependent on the salt used and its concentration, such that the UL42 protein may inhibit, have little or no effect on, or activate the Pol activity, depending on the condition used. The observed effects are due to the values for Km(app) for activated DNA and Vmaxapp for Pol and the Pol-UL42 protein complex differently varying with salt concentration.
Hart
G J
GJ
Department of Virology, Glaxo Group Research Ltd., Greenford, Middlesex, UK.
Boehme
R E
RE
D000645:Ammonium Sulfate / Q000378:metabolism; D002478:Cells, Cultured; D004247:DNA / Q000378:metabolism; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D005090:Exodeoxyribonucleases; D007700:Kinetics; D011189:Potassium Chloride / Q000378:metabolism; D018139:Simplexvirus / Q000201:enzymology*; D012965:Sodium Chloride / Q000378:metabolism; D014764:Viral Proteins / Q000378:metabolism*
D014764:Viral Proteins; D012965:Sodium Chloride; D011189:Potassium Chloride; D004247:DNA; D004259:DNA-Directed DNA Polymerase; D005090:Exodeoxyribonucleases; C043417:DNA polymerase, Simplexvirus; D000645:Ammonium Sulfate