Cell growth effects of Epstein-Barr virus leader protein.
B lymphoblastoid cell lines immortalized with P3HR1/633 Epstein-Barr virus (EBV), which has a deletion in the EBV nuclear antigen leader protein (EBNA-LP) gene, were transfected with a vector expressing wild-type EBNA-LP. The EBNA-LP transfectants grew out faster under G418 selection than control cells but expression of EBNA-LP made no significant difference to growth rate or saturation density of the resulting established cell lines. When the cells expressing EBNA-LP were allowed to grow to saturation and then diluted in fresh medium they underwent DNA synthesis more rapidly than control cultures.
Allan
G J
GJ
Ludwig Institute for Cancer Research, St Mary's Hospital Medical School, London, U.K.
Inman
G J
GJ
Parker
B D
BD
Rowe
D T
DT
Farrell
P J
PJ
D000956:Antigens, Viral / Q000235:genetics*; D001402:B-Lymphocytes / Q000166:cytology / Q000382:microbiology*; D015153:Blotting, Western; D002455:Cell Division; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral; D019309:Epstein-Barr Virus Nuclear Antigens; D005455:Fluorescent Antibody Technique; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000276:immunology* / Q000502:physiology*; D006801:Humans; D014162:Transfection
D000956:Antigens, Viral; D019309:Epstein-Barr Virus Nuclear Antigens