Comparison of the Western immunoblot assay and a glycoprotein G enzyme immunoassay for detection of serum antibodies to herpes simplex virus type 2 in patients with AIDS.
Herpes simplex virus type 2 (HSV-2) seroprevalence in 68 patients with AIDS was 77% by Western blot (immunoblot) and 44% by glycoprotein G-2 immunoassay. Each of 16 patients with culture-proven HSV-2 infection was positive by Western blot versus 8 by glycoprotein G-2 immunoassay. No differences in age, race, duration of AIDS, acyclovir usage, or HSV-1 seroprevalence were found to explain differences in sensitivity.
Safrin
S
S
Department of Medicine, University of California, San Francisco 94143.
Arvin
A
A
Mills
J
J
Ashley
R
R
D000163:Acquired Immunodeficiency Syndrome / Q000382:microbiology*; D000914:Antibodies, Viral / Q000032:analysis*; D015153:Blotting, Western; D005260:Female; D006801:Humans; D007118:Immunoassay; D008297:Male; D018139:Simplexvirus / Q000276:immunology*; D014759:Viral Envelope Proteins / Q000276:immunology*
D000914:Antibodies, Viral; D014759:Viral Envelope Proteins; C045464:glycoprotein G, herpes simplex virus type 2