title: Introduction of multiple restriction enzyme sites by in vitro mutagenesis using the polymerase chain reaction.
authors.affiliation: Department of Pathology, University of Pittsburgh School of Medicine, Pennsylvania 15261.
authors.forename: B
authors.initials: B
authors.lastname: Shankarappa
authors.forename: R
authors.initials: R
authors.lastname: Balachandran
authors.forename: P
authors.initials: P
authors.lastname: Gupta
authors.forename: G D
authors.initials: GD
authors.lastname: Ehrlich
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000737:chemistry; D004262:DNA Restriction Enzymes; D015752:Genes, env; D015497:HIV-1 / Q000235:genetics; D008969:Molecular Sequence Data; D016296:Mutagenesis; D016133:Polymerase Chain Reaction; D013379:Substrate Specificity
chemical_list: D004247:DNA; D004262:DNA Restriction Enzymes