[Lectin-induced chemiluminescence of peripheral blood neutrophils in patients with ischemic heart disease before and after blood irradiation with helium-neon laser].
The blood taken from 35 patients with coronary heart disease and 30 healthy donors was irradiated with He-Ne laser, which resulted in a decrease in its count of segmented neutrophilic granulocytes. Lectins bound to various carbohydrate determinants onto the neutrophil surface were shown to affect changes occurring after luminol-depended chemiluminescence irradiation in patients and healthy persons in different ways. The patients' neutrophils contained lower levels of radiommunologically detectable leukotriene B4. Thromboxane B2 levels also dropped following the irradiation. The laser irradiation induced elimination of some less resistant cells from blood flow, i.e. "rejuvenation" of a cell population of neutrophilic granulocytes. The remaining cells differed in the composition and reactivity of surface lectin receptors and in the content of biologically active substances, which is likely to play the key role in the mechanism responsible for the therapeutical effect of He-Ne laser.
Kipshidze
N N
NN
Khomeriki
S G
SG
Kubatiev
A A
AA
Korochkin
I M
IM
Denisov
S M
SM
Shliapnikov
V N
VN
D003327:Coronary Disease / Q000097:blood* / Q000532:radiotherapy; D005260:Female; D006371:Helium; D006801:Humans; D066298:In Vitro Techniques; D053685:Laser Therapy; D037102:Lectins / Q000008:administration & dosage*; D007975:Leukotriene B4 / Q000097:blood / Q000737:chemistry; D008163:Luminescent Measurements; D008297:Male; D009356:Neon; D009504:Neutrophils / Q000737:chemistry / Q000187:drug effects* / Q000528:radiation effects; D013929:Thromboxane B2 / Q000097:blood / Q000737:chemistry
D037102:Lectins; D007975:Leukotriene B4; D006371:Helium; D009356:Neon; D013929:Thromboxane B2
Lektinindutsirovannaia khemiliuminestsentsiia neĭtrofil'nykhgranu- lotsitov perifericheskoĭ krovi bol'nykh ishemicheskoĭ bolezn'iu serdtsa do i posle oblucheniia krovi geliĭ-neonovym lazerom.