Efficient electropulse transformation of intact Candida maltosa cells by different homologous vector plasmids.
Conditions for efficient and quick transformation by electroporation were developed in Candida maltosa. To investigate the efficiency of transformation with integrative as well as with autonomously replicating plasmids, a series of vectors was constructed for homologous transformation of this species. Transformants were obtained with different plasmids as covalently closed circular molecules and as linearized DNA. The influence of recipient strain and plasmid type as well as of cell number and parameters of the supplied electrical pulse on the transformation efficiency have been investigated. A maximum of 7000 transformants per 100 ng of plasmid DNA was reached. The efficiency of transformation was compared with that of the LiCl method.
Kasüske
A
A
Ernst-Moritz-Arndt-Universität Greifswald, Fachrichtung Biologie, Institut für Genetik, Germany.
Wedler
H
H
Schulze
S
S
Becher
D
D
D002175:Candida / Q000235:genetics*; D002712:Chlorides; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D004560:Electricity; D005069:Evaluation Studies as Topic; D005821:Genetic Techniques; D005822:Genetic Vectors; D008094:Lithium; D018021:Lithium Chloride; D009690:Nucleic Acid Conformation; D010957:Plasmids; D014170:Transformation, Genetic
D002712:Chlorides; D004271:DNA, Fungal; D008094:Lithium; D018021:Lithium Chloride