ATP-activated Ca(2+)-permeable channels in rat peritoneal macrophages.
The patch-clamp technique was used to study mechanisms of ATP-induced Ca2+ influx in rat peritoneal macrophages. The experiments on whole-cell and patch membranes have shown that extracellular ATP activates channels permeable to di- and monovalent inorganic cations. Ratios of unitary channel conductances in 105 mM Ca2+, Sr2+, Mn2+, Ba2+ and normal sodium solutions were 1.0, 0.95, 0.75, 0.55 and 0.85, respectively. The channels could open in the presence of non-hydrolyzable GTP analogues in artificial intracellular solution. The data are consistent with the hypothesis that a GTP-binding protein is involved in receptor-to-channel coupling.
Naumov
A P
AP
Institute of Cytology of Russian Academy of Sciences, St. Petersburg.
Kuryshev
Y A
YA
Kaznacheyeva
E V
EV
Mozhayeva
G N
GN
D000255:Adenosine Triphosphate / Q000502:physiology*; D000818:Animals; D002118:Calcium / Q000502:physiology*; D015220:Calcium Channels / Q000502:physiology*; D002413:Cations, Divalent; D002462:Cell Membrane / Q000502:physiology; D004553:Electric Conductivity / Q000187:drug effects; D016244:Guanosine 5'-O-(3-Thiotriphosphate) / Q000494:pharmacology; D066298:In Vitro Techniques; D015640:Ion Channel Gating; D008264:Macrophages / Q000502:physiology*; D010529:Peritoneal Cavity / Q000166:cytology; D051381:Rats
D015220:Calcium Channels; D002413:Cations, Divalent; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D000255:Adenosine Triphosphate; D002118:Calcium