High-performance liquid chromatographic determination of rufloxacin and its main active metabolite in biological fluids.
A high-performance liquid chromatographic method is described for the determination of a fluoroquinolone, rufloxacin, and its N-desmethyl metabolite in plasma, urine and bile. Samples are chromatographed on a poly(styrene-divinylbenzene) column, the eluate being monitored with a fluorescence detector. The method was validated and a detection limit of 10 ng/ml for both rufloxacin and its metabolite in all the biological matrices considered was found. The method was successfully applied in pharmacokinetic studies.
Lombardi
F
F
ISF, Laboratories for Biomedical Research, Milan, Italy.
Ardemagni
R
R
Colzani
V
V
Visconti
M
M
D000890:Anti-Infective Agents / Q000032:analysis* / Q000493:pharmacokinetics; D001646:Bile / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D003640:Dealkylation; D004355:Drug Stability; D024841:Fluoroquinolones; D006801:Humans; D015363:Quinolones / Q000032:analysis* / Q000493:pharmacokinetics; D013050:Spectrometry, Fluorescence
D000890:Anti-Infective Agents; D024841:Fluoroquinolones; D015363:Quinolones; C060328:rufloxacin