title: Effect of calcium on oxalate uptake and transport by the rat intestine.
abstract: The effect of calcium concentration (0-10 mmol 1(-1)) on oxalate uptake and transport was investigated in vitro using everted gut segments and sacs. Increase in calcium concentration in the incubation medium led to an increase in the amounts of precipitated oxalate on the intestine; however, the net oxalate flux to the serosal side decreased. The ions, i.e. Ca2+, Ox2-, H2PO4-, HPO4(2-), present in the incubation medium favoured formation of hydroxyapatite and calcium oxalate crystals, as evidenced by Equil II analysis and free energy of the system. The nature of precipitates was confirmed by elemental analysis, X-ray diffraction spectrometry and electron microscopy. Oxalate precipitated on the intestine following incubation with calcium could be released into a calcium- and oxalate-free medium. Animals fed oxalate in the absence and presence of calcium revealed that, during 1 h in the absence of calcium, oxalate moved down the intestinal tract as a distinct peak of greater than 50% (70-90 cm in the intestine), leaving less than 10% in the stomach and first 50 cm of the intestine. In the case of animals fed calcium along with oxalate, 35% of the oxalate was still present in the stomach, and the amounts of oxalate in the intestinal segments gradually increased from 4.5% to 21.7% (0-90 cm) and dropped to 2.1% in the next 20 cm. Since oxalaemia and oxaluria appear to be influenced by intestinal oxalate absorption, the present observations may help to improve understanding of the pathophysiology of disorders exhibiting altered oxalate metabolism.
authors.affiliation: Department of Surgery, University of Erlangen, Germany.
authors.forename: V
authors.initials: V
authors.lastname: Sharma
authors.forename: P O
authors.initials: PO
authors.lastname: Schwille
mesh_terms: D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D002118:Calcium / Q000008:administration & dosage / Q000494:pharmacology*; D003460:Crystallization; D017886:Durapatite; D005753:Gastric Mucosa / Q000378:metabolism; D006882:Hydroxyapatites / Q000378:metabolism; D066298:In Vitro Techniques; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000187:drug effects*; D008297:Male; D008871:Microvilli / Q000378:metabolism; D010070:Oxalates / Q000378:metabolism*; D019815:Oxalic Acid; D051381:Rats; D011919:Rats, Inbred Strains; D013270:Stomach / Q000187:drug effects
chemical_list: D006882:Hydroxyapatites; D010070:Oxalates; D017886:Durapatite; D019815:Oxalic Acid; D002118:Calcium