title: Sequence analysis of WIS-2-1A, a retrotransposon-like element from wheat.
abstract: WIS-2-1A, a 8624 bp insertion in the Glu-1A-2 locus of chromosome 1A of wheat, consists of two 1755 bp long terminal repeats enclosing a 5114 bp internal region. No long open reading frames could be found, but inspection of the predicted amino acid sequence showed regions with homology to retrotransposon structures, including a methionine tRNA initiator binding site, a nucleotide binding domain, a protease, an integrase and a polymerase. DNA replication errors have resulted in frame-shifts in the protein coding region, suggesting that retrotransposition of WIS-2-1A, if it occurs, must be mediated by trans-acting factors.
authors.affiliation: Institute for Plant Science Research, John Innes Institute, Norwich, UK.
authors.forename: G J
authors.initials: GJ
authors.lastname: Murphy
authors.forename: H
authors.initials: H
authors.lastname: Lucas
authors.forename: G
authors.initials: G
authors.lastname: Moore
authors.forename: R B
authors.initials: RB
authors.lastname: Flavell
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D001665:Binding Sites; D002874:Chromosome Mapping; D003062:Codon; D004254:DNA Nucleotidyltransferases / Q000235:genetics*; D004261:DNA Replication; D004251:DNA Transposable Elements; D004259:DNA-Directed DNA Polymerase / Q000235:genetics; D016368:Frameshift Mutation; D019426:Integrases; D008969:Molecular Sequence Data; D012091:Repetitive Sequences, Nucleic Acid; D012190:Retroviridae / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D014908:Triticum / Q000235:genetics*
chemical_list: D003062:Codon; D004251:DNA Transposable Elements; D004254:DNA Nucleotidyltransferases; D019426:Integrases; D004259:DNA-Directed DNA Polymerase