Detection and characterization of hepatitis C virus sequence in the serum of a patient with chronic HCV infection.
A cDNA fragment corresponding to the nonstructural gene region of Hepatitis C virus was cloned and sequenced. cDNA was obtained by reverse transcription of viral RNA extracted from serum of a German patient with chronic post transfusion hepatitis. "Nested" PCR resulted in a cDNA fragment of 345 nt. The sequence showed a homology of 96% to the American prototype HCV.
Schreier
E
E
Robert Koch-Institute of the Federal Health Office, Berlin, Federal Republic of Germany.
Fuchs
K
K
Höhne
M
M
Motz
M
M
Zachoval
R
R
Esteban
J
J
Dittmann
S
S
Deinhardt
F
F
Roggendorf
M
M
D001483:Base Sequence; D005814:Genes, Viral / Q000235:genetics*; D016174:Hepacivirus / Q000235:genetics*; D006526:Hepatitis C / Q000453:epidemiology / Q000235:genetics*; D006521:Hepatitis, Chronic / Q000453:epidemiology / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D012367:RNA, Viral / Q000097:blood*; D012689:Sequence Homology, Nucleic Acid
D012367:RNA, Viral