title: Differential response of preadipocytes and adipocytes to prostacyclin and prostaglandin E2: physiological implications.
abstract: The major prostaglandins (PGs) locally produced in adipose tissue both in rodent and man are PGE2 and prostacyclin (PGI2). We have recently described PGI2 as an autocrine promoter and/or amplifier of terminal differentiation of cultured preadipocytes in several species. The effectiveness and specificity of PGI2 as an adipogenic agent are related to its ability to induce in preadipocytes intracellular increases of both cAMP and free calcium. Moreover, PGs of the E series are well known to exert an antilipolytic effect in mature adipocytes. These observations have prompted us to address two questions of physiological interest: 1) Is PGI2 still able to increase cAMP in differentiated adipocytes, behaving thus as a lipolytic agent, and 2) Is PGE2 able to negatively modulate cAMP production in adipose precursor cells, behaving thus as a counteracting effector of PGI2 action? Our results, with respect to cAMP production and/or lipolysis and antilipolysis, demonstrate clearly that in adipose tissue of both rat and man, PGI2 exclusively affects adipose precursor cells whereas PGE2 exclusively affects adipocytes. We propose a model of concerted action for both PGs in the development of adipose tissue mass, PGI2 behaving as an adipogenic-hyperplastic effector and PGE2 as an antilipolytic-hypertrophic effector.
authors.affiliation: Centre de Biochimie du CNRS (UMR 134), Université de Nice-Sophia Antipolis, Faculté des Sciences, Parc Valrose, Nice, France.
authors.forename: G
authors.initials: G
authors.lastname: Vassaux
authors.forename: D
authors.initials: D
authors.lastname: Gaillard
authors.forename: C
authors.initials: C
authors.lastname: Darimont
authors.forename: G
authors.initials: G
authors.lastname: Ailhaud
authors.forename: R
authors.initials: R
authors.lastname: Negrel
mesh_terms: D000273:Adipose Tissue / Q000166:cytology* / Q000378:metabolism / Q000502:physiology*; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000378:metabolism / Q000502:physiology; D015232:Dinoprostone / Q000494:pharmacology*; D011464:Epoprostenol / Q000494:pharmacology*; D005260:Female; D006801:Humans; D050356:Lipid Metabolism; D008297:Male; D008954:Models, Biological; D051381:Rats; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism / Q000502:physiology*
chemical_list: D011464:Epoprostenol; D000242:Cyclic AMP; D015232:Dinoprostone; D002118:Calcium