Recombinant polypeptide from the gp48 region of the bovine viral diarrhea virus (BVDV) detects serum antibodies in vaccinated and infected cattle.
To characterize the immune response of cattle to bovine viral diarrhea virus (BVDV) glycoprotein gp48, we have produced a large amount of recombinant glutathione-s-transferase-gp48 (GST-gp48) fusion protein in Escherichia coli. Antibodies to gp48 were present in cattle vaccinated with killed or modified-live virus vaccination, or following natural infection. These results were in agreement with results of serum neutralization (SN) test which detected gp53 of BVDV.
Kwang
J
J
USDA, ARS, U.S. Meat Animal Research Center, Clay Center, NE 68933.
Littledike
E T
ET
Donis
R O
RO
Dubovi
E J
EJ
D000595:Amino Acid Sequence; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D001483:Base Sequence; D015153:Blotting, Western; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000276:immunology*; D002417:Cattle; D001908:Diarrhea Viruses, Bovine Viral / Q000276:immunology*; D015967:Gene Expression Regulation, Viral; D005822:Genetic Vectors; D005982:Glutathione Transferase / Q000737:chemistry; D006023:Glycoproteins / Q000737:chemistry / Q000276:immunology*; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D011993:Recombinant Fusion Proteins / Q000737:chemistry; D014611:Vaccination / Q000662:veterinary; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000276:immunology*; D014765:Viral Vaccines / Q000276:immunology*
D000914:Antibodies, Viral; D006023:Glycoproteins; D011993:Recombinant Fusion Proteins; D014759:Viral Envelope Proteins; D014765:Viral Vaccines; C078531:glycoprotein 48, bovine viral diarrhea virus; D005982:Glutathione Transferase