title: Ca(2+)-dependent translocation of cytosolic proteins to isolated granule subpopulations and plasma membrane from human neutrophils.
abstract: In order to identify cytosolic proteins involved in control of granule exocytosis in human neutrophils, subcellular fractions enriched in each of the 3 major granule subsets were incubated with cytosol from neutrophils in the presence or absence of Ca2+. After washing, proteins were eluted from the organelles by EGTA. Annexins I, II, IV and VI were found to bind to all organelles studied. In addition, a 28-kDa protein was found to bind exclusively to plasma membranes and secretory vesicles, the most readily exocytosed organelle of neutrophils. Ca(2+)-dependent association of cytosolic proteins to different granule subsets may control differential exocytosis of granules.
authors.affiliation: Department of Hematology, University Hospital, Copenhagen, Denmark.
authors.forename: N
authors.initials: N
authors.lastname: Borregaard
authors.forename: L
authors.initials: L
authors.lastname: Kjeldsen
authors.forename: K
authors.initials: K
authors.lastname: Lollike
authors.forename: H
authors.initials: H
authors.lastname: Sengeløv
mesh_terms: D001693:Biological Transport, Active; D002118:Calcium / Q000494:pharmacology*; D002462:Cell Membrane / Q000378:metabolism*; D003594:Cytoplasmic Granules / Q000187:drug effects / Q000378:metabolism*; D003600:Cytosol / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*; D005089:Exocytosis / Q000187:drug effects; D018093:Gelatinases; D006801:Humans; D009504:Neutrophils / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*; D010434:Pepsin A / Q000032:analysis; D013347:Subcellular Fractions / Q000201:enzymology
chemical_list: D010434:Pepsin A; D018093:Gelatinases; D002118:Calcium