Carriers of the mouse rd gene have reduced levels of the beta subunit of the retinal cyclic GMP phosphodiesterase.
Polyclonal antipeptide antisera have been utilized to quantitate the amount of retinal rod outer segment cGMP phosphodiesterase alpha and beta catalytic subunits present in retinas from C57BL/6J mice which are normal or carriers for the rd gene defect. Results suggest that the quantity of PDE-beta subunit is reduced in carrier mice while PDE-alpha and PDE-gamma are not affected. In 21-day-old mice, the PDE-beta was reduced by about one-half while adult carrier mice had even more reduced levels of PDE-beta. Since PDE alpha was not reduced, this suggests that synthesis of PDE alpha and PDE beta may not be coordinately controlled.
Cunnick
J
J
Department of Anatomy and Physiology, Kansas State University, Manhattan 66506.
Takemoto
D J
DJ
D015106:3',5'-Cyclic-GMP Phosphodiesterases / Q000032:analysis / Q000235:genetics* / Q000378:metabolism; D000375:Aging; D000818:Animals; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D005796:Genes; D006580:Genetic Carrier Screening; D006720:Homozygote; D007106:Immune Sera; D046911:Macromolecular Substances; D051379:Mice; D008810:Mice, Inbred C57BL; D012160:Retina / Q000254:growth & development; D012374:Rod Cell Outer Segment / Q000201:enzymology*
D007106:Immune Sera; D046911:Macromolecular Substances; D015106:3',5'-Cyclic-GMP Phosphodiesterases