Mapping of a human brain voltage-gated calcium channel to human chromosome 12p13-pter.
Degenerate DNA oligomers coding for highly conserved regions of the voltage-gated calcium channel were synthesized for the polymerase chain reaction (PCR) using DNA from a human brain cDNA library as template. PCR amplified a 640-bp DNA fragment from the human brain cDNA library. Sequencing revealed that this fragment encodes part of a protein highly homologous to a subtype of the dihydropyridine-sensitive calcium channel cloned from rabbit heart and rat brain. Southern analysis of panels of somatic cell hybrids mapped the 640-bp fragment, CACNL1A1, to human chromosome 12p13-pter.
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W
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Department of Biology, University of California San Diego, La Jolla 92093-0319.
McPherson
J D
JD
Hoang
D Q
DQ
Wasmuth
J J
JJ
Evans
G A
GA
Montal
M
M
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D001921:Brain / Q000378:metabolism*; D015220:Calcium Channels / Q000235:genetics*; D020746:Calcium Channels, L-Type; D002874:Chromosome Mapping; D002881:Chromosomes, Human, Pair 12; D006224:Cricetinae; D004247:DNA; D006801:Humans; D006822:Hybrid Cells; D015640:Ion Channel Gating; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics*; D016133:Polymerase Chain Reaction
C079146:CACNA1C protein, human; D015220:Calcium Channels; D020746:Calcium Channels, L-Type; D009419:Nerve Tissue Proteins; D004247:DNA