title: Transcriptional activity of HIV-1 and HHV-6 in retinal lesions from AIDS patients.
abstract: This study determined the frequency of multiple viral (HIV-1, HHV-6, and CMV) infections in 26 retinas from 16 AIDS patients. Of the 12 retinas of 26 that tested positive for HIV-1 DNA sequences, seven also were positive for HHV-6 DNA sequences. Four of these seven retinas were culture positive for HIV-1 and two of the four contained CMV DNA sequences and antigens. Using RNA probes, HIV-1 and HHV-6 transcriptional activity was demonstrated in two of the four HIV-1 culture positive retinas. These retinas also contained CMV DNA sequences and antigens. The results demonstrate that more than 35% of AIDS patients suffer from at least two simultaneous viral infections and 15% suffer from three viral infections. The presence of transcriptional activity of HIV-1 and HHV-6 suggests an active infection.
authors.affiliation: Division of Molecular Virology, Baylor College of Medicine, Houston, Texas 77030.
authors.forename: H B
authors.initials: HB
authors.lastname: Qavi
authors.forename: M T
authors.initials: MT
authors.lastname: Green
authors.forename: G K
authors.initials: GK
authors.lastname: SeGall
authors.forename: D E
authors.initials: DE
authors.lastname: Lewis
authors.forename: F B
authors.initials: FB
authors.lastname: Hollinger
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000382:microbiology / Q000473:pathology; D000914:Antibodies, Viral / Q000032:analysis; D001483:Base Sequence; D003587:Cytomegalovirus / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification / Q000648:ultrastructure; D003586:Cytomegalovirus Infections / Q000382:microbiology / Q000473:pathology; D004279:DNA, Viral / Q000235:genetics; D015828:Eye Infections, Viral / Q000382:microbiology*; D015967:Gene Expression Regulation, Viral; D015658:HIV Infections / Q000382:microbiology*; D015497:HIV-1 / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification / Q000648:ultrastructure; D006566:Herpesviridae Infections / Q000382:microbiology*; D015654:Herpesvirus 6, Human / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification / Q000648:ultrastructure; D006801:Humans; D008969:Molecular Sequence Data; D015347:RNA Probes; D012164:Retinal Diseases / Q000382:microbiology* / Q000473:pathology
chemical_list: D000914:Antibodies, Viral; D004279:DNA, Viral; D015347:RNA Probes