Evidence that Ha-Ras mediates two distinguishable intracellular signals activated by v-Src.
v-Src activates promoters under the control of 12-O-tetradecanoylphorbol-13-acetate (TPA) response elements (TREs) and serum response elements (SREs) via two distinguishable intracellular signaling mechanisms. The induction of TRE- and SRE-mediated gene expression by v-Src could be distinguished by a differential sensitivity to depleting cells of protein kinase C (PKC) and to a dominant negative Raf-1 mutant. Thus, PKC depletion and the dominant negative Raf-1 mutant were able to distinguish two intracellular signaling mechanisms activated by v-Src. Both of these v-Src-induced intracellular signals were sensitive to a dominant negative mutant of Ha-Ras. These data suggest that Ha-Ras functions to coordinately regulate multiple intracellular signaling mechanisms activated by v-Src.
Qureshi
S A
SA
Institute for Biomolecular Structure and Function, Hunter College, City University of New York, New York 10021.
Alexandropoulos
K
K
Rim
M
M
Joseph
C K
CK
Bruder
J T
JT
Rapp
U R
UR
Foster
D A
DA
D016475:3T3 Cells; D000818:Animals; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics / Q000378:metabolism; D003001:Cloning, Molecular; D020690:GTPase-Activating Proteins; D005786:Gene Expression Regulation; D011905:Genes, ras; D016391:Genes, src; D051379:Mice; D010957:Plasmids; D011401:Promoter Regions, Genetic / Q000187:drug effects; D011506:Proteins / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D015183:Restriction Mapping; D018139:Simplexvirus / Q000201:enzymology / Q000235:genetics; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D013937:Thymidine Kinase / Q000235:genetics; D014158:Transcription, Genetic; D014162:Transfection; D020703:ras GTPase-Activating Proteins
D020690:GTPase-Activating Proteins; D011506:Proteins; D011994:Recombinant Proteins; D020703:ras GTPase-Activating Proteins; D015500:Chloramphenicol O-Acetyltransferase; D013937:Thymidine Kinase; D013755:Tetradecanoylphorbol Acetate