Androgen-responsive expression and mitogenic activity of schwannoma-derived growth factor on an androgen-dependent Shionogi mouse mammary carcinoma cell line.
We report here, using the androgen-dependent SC2G cell line derived from Shionogi mouse mammary carcinoma SC115, that cDNA encoding mouse homologue of the rat schwannoma-derived growth factor (SDGF) was isolated and its protein product was identified to be an autocrine growth factor which is expressed in response to androgen in this hormone-dependent cell line. The androgen-independent SC1G cells derived from SC115 were shown to express SDGF even in the absence of androgen, while Northern analysis probed with mouse mammary tumor virus (MMTV) DNA showed that the androgen inducible transcriptional machinery remains functional in this hormone independent cell line.
Sonoda
H
H
Shionogi Research Laboratories, Shionogi & Co., Ltd., Osaka, Japan.
Yamaguchi
T
T
Watanabe
S
S
D000595:Amino Acid Sequence; D066258:Amphiregulin; D000728:Androgens / Q000494:pharmacology; D000818:Animals; D001483:Base Sequence; D002455:Cell Division; D066255:EGF Family of Proteins; D006023:Glycoproteins; D006133:Growth Substances / Q000096:biosynthesis* / Q000494:pharmacology; D036341:Intercellular Signaling Peptides and Proteins; D008325:Mammary Neoplasms, Experimental / Q000378:metabolism*; D008324:Mammary Tumor Virus, Mouse / Q000235:genetics; D051379:Mice; D008934:Mitogens / Q000494:pharmacology*; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000096:biosynthesis; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured
D066258:Amphiregulin; D000728:Androgens; C587849:Areg protein, mouse; C587850:Areg protein, rat; D066255:EGF Family of Proteins; D006023:Glycoproteins; D006133:Growth Substances; D036341:Intercellular Signaling Peptides and Proteins; D008934:Mitogens; D012333:RNA, Messenger