title: The complete sequence of African horsesickness virus serotype 4 (vaccine strain) RNA segment 5 and its predicted polypeptide compared with NS1 of bluetongue virus.
abstract: The complete sequence of RNA segment 5 of the African horsesickness virus serotype 4 (AHSV-4) vaccine strain was determined from cDNA clones inserted into pBR322. The RNA is 1751 bp long (M(r) 1.12 x 10(6)) and contains an open reading frame encoding a protein of 548 amino acids (M(r) 63,122) with a net charge of +0.5 at neutral pH. A comparison of the sequence of AHSV-4 segment 5 with that of segment 6 of bluetongue virus (BTV) serotypes 10 and 17 revealed 49.2% and 48.9% nucleotide similarity, respectively, and 31.4% amino acid similarity. However, AHSV-4 segment 5 has no significant similarity to BTV segment 5. In addition, Northern blot hybridization showed that full-length AHSV-4 segment 5 cDNA cross-hybridized with the corresponding genes of all serotypes of attenuated AHSV.
authors.affiliation: Exotic Disease Research Division, National Institute of Animal Health, Tokyo, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Mizukoshi
authors.forename: K
authors.initials: K
authors.lastname: Sakamoto
authors.forename: A
authors.initials: A
authors.lastname: Iwata
authors.forename: T
authors.initials: T
authors.lastname: Tsuchiya
authors.forename: S
authors.initials: S
authors.lastname: Ueda
authors.forename: T
authors.initials: T
authors.lastname: Watanabe
authors.forename: M
authors.initials: M
authors.lastname: Kamada
authors.forename: A
authors.initials: A
authors.lastname: Fukusho
mesh_terms: D000356:African Horse Sickness Virus / Q000145:classification / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D001820:Bluetongue virus / Q000145:classification / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D017385:Sequence Homology; D012703:Serotyping; D017361:Viral Nonstructural Proteins / Q000145:classification / Q000235:genetics*; D014765:Viral Vaccines
chemical_list: D017361:Viral Nonstructural Proteins; D014765:Viral Vaccines