[The expression of kainate and GABA receptors in oocytes of Bufo bufo gargarizans].
Oocytes of Bufo bufo gargarizans were used as an expression system for analyzing structure of exogenous membrane protein and their functions. Poly (A)+ mRNA isolated from rat brain was injected into Toad (Bufo bufo gargarizans) oocytes (50 ng/oocyte). Rat brain kainic acid and gamma-aminobutyric acid(GABA) receptors expressed by the injected mRNA were integrated in the oocyte membrane 48 h after the injection at 19 degrees C. Membrane currents induced by kainic acid (5 x 10(-5) mol/L) and GABA (10(-4) mol/L) were 294.0 +/- 6.4 nA (n = 5) and 309.5 +/- 4.9 nA (n = 4) respectively. The kainic acid induced current reached its maximum value at about 10(-3) mol/L. Moreover, it was observed that the 36Cl- influx of the oocytes microinjected with mRNA was one-fold more rapid than the control oocytes. These results indicate that the oocytes of Bufo bufo gargarizans as those of Xenopus laevis can be used to express membrane proteins (receptors & transports) to acquire their proper functions from exogenous mRNA.
Gu
Q B
QB
Shanghai Institute of Cell Biology, Shanghai Joint Laboratory of Life Science, Academia Sinica.
Zhu
H
H
Li
W
W
Yao
Y
Y
Bao
Y D
YD
Tso
J K
JK
Yang
X L
XL
D000818:Animals; D001693:Biological Transport, Active; D002023:Bufo bufo; D002712:Chlorides / Q000378:metabolism; D009865:Oocytes / Q000378:metabolism / Q000502:physiology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D011963:Receptors, GABA-A / Q000096:biosynthesis* / Q000235:genetics; D017470:Receptors, Glutamate / Q000096:biosynthesis* / Q000235:genetics; D018092:Kainic Acid Receptors
D002712:Chlorides; D012333:RNA, Messenger; D011963:Receptors, GABA-A; D017470:Receptors, Glutamate; D018092:Kainic Acid Receptors