On the mechanism of the interaction of ketamine and halothane in vitro.
1. Electrically induced contraction of guinea pig ileum myenteric plexus-longitudinal muscle was inhibited by ketamine and halothane with IC50s of 2.1 x 10(-4) M and 1.8 v/v% respectively. 2. The inhibitory action of ketamine was partially antagonized by naloxone and the selective kappa antagonist nor-binaltorphimine. 3. The actions of ketamine and halothane were synergistic at high levels of response (above 30% inhibition). 4. The actions of ketamine and halothane became antagonistic after treatment with pertussis toxin. 5. The interaction of ketamine and halothane was similar to the interaction of morphine and halothane.
Bansinath
M
M
Department of Anesthesiology, New York University Medical Center, NY.
Warner
W
W
Tang
C K
CK
Turndorf
H
H
Puig
M M
MM
D000818:Animals; D004347:Drug Interactions; D019204:GTP-Binding Proteins / Q000502:physiology; D006168:Guinea Pigs; D006221:Halothane / Q000494:pharmacology*; D007082:Ileum / Q000187:drug effects; D066298:In Vitro Techniques; D007649:Ketamine / Q000494:pharmacology*; D009130:Muscle, Smooth / Q000187:drug effects; D009131:Muscle, Smooth, Vascular / Q000187:drug effects; D009197:Myenteric Plexus / Q000187:drug effects; D009270:Naloxone / Q000494:pharmacology; D009271:Naltrexone / Q000031:analogs & derivatives / Q000494:pharmacology; D037342:Pertussis Toxin; D011957:Receptors, Opioid / Q000187:drug effects; D010566:Virulence Factors, Bordetella / Q000494:pharmacology
D011957:Receptors, Opioid; D010566:Virulence Factors, Bordetella; D009270:Naloxone; C051844:norbinaltorphimine; D009271:Naltrexone; D007649:Ketamine; D037342:Pertussis Toxin; D019204:GTP-Binding Proteins; D006221:Halothane