title: [Contribution of immunohistochemistry and in situ hybridization techniques in diseases caused by or associated with Epstein-Barr virus].
abstract: Epstein-Barr virus is associated with many diseases. Today, the pathologist may study either by immunohistochemistry or in situ hybridization on tissue sections: EBV genome, EBV messenger RNA, EBV latent and replicative proteins. Several technics can be performed on fixed paraffin-embedded tissue to demonstrate the presence of EBV DNA, EBER-1 RNA, LMP-1 protein. Frozen tissues are required for the study of EBNA-2, ZEBRA and replicating proteins expression. The results, obtained during the study of benign and malignant proliferations always or often associated with EBV, such as infectious mononucleosis, Burkitt's lymphomas, AIDS associated lymphomas, lymphoproliferations in immunocompromised patients, Hodgkin's disease, and some epithelial proliferations, are summarized.
authors.affiliation: Service Central Jacques-Delarue d'Anatomie et de Cytologie Pathologiques, Hôtel-Dieu de Paris.
authors.forename: J
authors.initials: J
authors.lastname: Audouin
authors.forename: S
authors.initials: S
authors.lastname: Prevot
authors.forename: J P
authors.initials: JP
authors.lastname: Brouland
authors.forename: T
authors.initials: T
authors.lastname: Molina
authors.forename: A
authors.initials: A
authors.lastname: Le Tourneau
authors.forename: J
authors.initials: J
authors.lastname: Diebold
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000150:complications; D002051:Burkitt Lymphoma / Q000150:complications / Q000175:diagnosis; D004279:DNA, Viral / Q000235:genetics*; D004854:Herpesvirus 4, Human / Q000302:isolation & purification; D006689:Hodgkin Disease / Q000175:diagnosis; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D017403:In Situ Hybridization; D007244:Infectious Mononucleosis / Q000175:diagnosis; D008223:Lymphoma / Q000150:complications; D008228:Lymphoma, Non-Hodgkin / Q000175:diagnosis; D008232:Lymphoproliferative Disorders / Q000175:diagnosis; D009693:Nucleic Acid Hybridization / Q000502:physiology; D012367:RNA, Viral / Q000235:genetics*
chemical_list: D004279:DNA, Viral; D012367:RNA, Viral
vernacular_title: Apport des techniques d'immunohistochimie et d'hybridation in situ dans les affections dues ou associées au virus d'Epstein-Barr.