Coxsackie B1 virus-induced changes in cell membrane-associated functions are not responsible for altered sensitivity to bacterial invasiveness.
To analyze the possible mechanisms by which coxsackie B1 virus infection affects the invasiveness of Shigella flexneri, we have studied the influence of intracellular levels of Na+ and K+, ATPase activity, cytoplasmic membrane potential, cAMP level and cell communication through gap junctions. 3h after adsorption of viable or UV-inactivated coxsackie B1 virus the Na(+)-K+ gradient of the cell collapsed, ATPase activity decreased, the cytoplasmic membranic potential-dependent tetraphosphonium ion uptake were reduced. No changes in cAMP or intercellular cell communication were observed. S. flexneri invasiveness in HEp-2 cell pretreated with viable or UV-inactivated coxsackie B 1 virus was enhanced, but bacterial invasiveness was unchanged in K(+)-depleted HEp-2 cells, cell cultures with high intracellular Na+ content or ouabain pre-treated cells compared to control cells. We found no correlation between the enhanced bacterial invasiveness in the early phase of coxsackie B 1 virus infection in HEp-2 cell cultures and intracellular K+ depletion, high intracellular Na+ content, inhibited Na(+)-K+ ATPase activity or membranic depolarization.
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Kaptein W. Wilhelmsen og Frues Bakteriologiske Institutt, University of Oslo, Norway.
Bukholm
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Mikalsen
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D002450:Cell Communication; D002460:Cell Line; D002462:Cell Membrane / Q000382:microbiology* / Q000502:physiology; D000242:Cyclic AMP / Q000378:metabolism; D029822:Enterovirus B, Human / Q000502:physiology* / Q000528:radiation effects; D007365:Intercellular Junctions / Q000378:metabolism; D008564:Membrane Potentials; D009861:Onium Compounds / Q000378:metabolism; D009943:Organophosphorus Compounds / Q000378:metabolism; D010042:Ouabain / Q000494:pharmacology; D011188:Potassium / Q000378:metabolism; D012763:Shigella flexneri / Q000502:physiology*; D015398:Signal Transduction; D012964:Sodium / Q000378:metabolism; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D014466:Ultraviolet Rays
D009861:Onium Compounds; D009943:Organophosphorus Compounds; D010042:Ouabain; D012964:Sodium; D000242:Cyclic AMP; D000254:Sodium-Potassium-Exchanging ATPase; D011188:Potassium; C013289:tetraphenylphosphonium