The basic region of AP-1 specifies glucocorticoid receptor activity at a composite response element.
Unrelated factors collaborate at composite response elements to confer novel patterns of transcriptional regulation. For example, AP-1 and glucocorticoid receptor bind and mutually affect their activities at a 25-bp composite element, plfG. We found that different members of the AP-1 factor family that behave similarly in the absence of receptor are strikingly distinct in its presence: They specify opposite (enhancement vs. repression) regulatory actions by the receptor. Four amino acids within the AP-1 DNA-binding domain were identified as crucial determinants of receptor regulatory activity at plfG. We conclude that interactions of factors from separate transcription factor families at composite response elements provide a mechanism by which a single factor can regulate both positively or negatively, and a potential resolution of the apparent functional redundancy within regulatory factor families.
Miner
J N
JN
Department of Biochemistry and Biophysics, University of California at San Francisco 94143-0448.
Yamamoto
K R
KR
D000595:Amino Acid Sequence; D001483:Base Sequence; D004247:DNA; D006023:Glycoproteins / Q000235:genetics; D036341:Intercellular Signaling Peptides and Proteins; D008969:Molecular Sequence Data; D011233:Precipitin Tests; D011388:Prolactin; D016755:Proto-Oncogene Proteins c-jun / Q000378:metabolism*; D011965:Receptors, Glucocorticoid / Q000378:metabolism*; D012045:Regulatory Sequences, Nucleic Acid; D014407:Tumor Cells, Cultured
D006023:Glycoproteins; D036341:Intercellular Signaling Peptides and Proteins; C000634106:Prl2c2 protein, mouse; D016755:Proto-Oncogene Proteins c-jun; D011965:Receptors, Glucocorticoid; D011388:Prolactin; D004247:DNA