Recombinant antigens in viral diagnosis.
DNA-fragments coding for a variety of different viral antigens have been cloned and expressed in E. coli. Selected purified recombinant antigens were used for detection of specific antibodies by the means of ELISA technique. This approach has been used for the development of four different ELISA's for the detection of HIV- and EBV-specific antibodies.
Vornhagen
R
R
Biotest AG, Research Department, Dreiechenhain, FRG.
Baur
A
A
Jahn
G
G
Hinderer
W
W
Nebel-Schickel
H
H
Horn
J
J
Wolf
H
H
Sonneborn
H H
HH
D000914:Antibodies, Viral / Q000032:analysis*; D000918:Antibody Specificity / Q000276:immunology; D000956:Antigens, Viral / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D015483:HIV Antibodies / Q000032:analysis; D015488:HIV Antigens; D015658:HIV Infections / Q000175:diagnosis / Q000276:immunology; D015497:HIV-1 / Q000276:immunology; D004854:Herpesvirus 4, Human / Q000276:immunology; D006801:Humans; D007244:Infectious Mononucleosis / Q000175:diagnosis / Q000276:immunology; D011994:Recombinant Proteins; D014412:Tumor Virus Infections / Q000175:diagnosis / Q000276:immunology; D014777:Virus Diseases / Q000175:diagnosis* / Q000276:immunology
D000914:Antibodies, Viral; D000956:Antigens, Viral; D015483:HIV Antibodies; D015488:HIV Antigens; D011994:Recombinant Proteins