title: Circulating immune complexes in bovine leukemia virus (BLV)-infected cattle.
abstract: Circulating immune complexes (ICs) were detected in the sera of bovine leukemia virus (BLV)-seropositive cattle. Immune complexes were precipitated in 2.5% polyethylene glycol (PEG) and further dissociated. Bovine leukemia virus antigens, IgG and IgM molecules were detected after solubilization in the presence of sodium dodecyl sulphate, and quantitated by enzyme-linked immunosorbent assay (ELISA) assays. Mean values of IgG and IgM in BLV-containing ICs did not significantly differ from those obtained from ICs originating from BLV-seronegative animals. However, differences were found in the composition of ICs from older BLV-positive animals as compared to those obtained from young animals. The ratio of IgG/IgM was 5.02 in animals aged 5-10 years, while this ratio was 11.66 in animals of less than 5 years of age and 10.19 in controls. This might indicate a possible increase in the contribution of IgM molecules to the structural composition of ICs in BLV-infected cattle as related to age or stage of infection.
authors.affiliation: Department of Immunology, Kimron Veterinary Institute, Beit Dagan, Israel.
authors.forename: H
authors.initials: H
authors.lastname: Ungar-Waron
authors.forename: J
authors.initials: J
authors.lastname: Brenner
authors.forename: R
authors.initials: R
authors.lastname: Paz
authors.forename: Z
authors.initials: Z
authors.lastname: Trainin
mesh_terms: D000375:Aging / Q000276:immunology; D000818:Animals; D000936:Antigen-Antibody Complex / Q000097:blood*; D002417:Cattle; D016583:Enzootic Bovine Leukosis / Q000276:immunology*; D005260:Female; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D001909:Leukemia Virus, Bovine / Q000276:immunology*
chemical_list: D000936:Antigen-Antibody Complex; D007074:Immunoglobulin G; D007075:Immunoglobulin M