title: Buried water in homologous serine proteases.
abstract: Buried water molecules in the structurally homologous family of eukaryotic serine proteases were examined to determine whether buried waters and their protein environments are conserved in these proteins. We found 16 equivalent water sites conserved in trypsin/ogen, chymotrypsin/ogen, elastase, kallikrein, thrombin, rat tonin and rat mast cell protease, and 5 additional water sites in enzymes which share the primary specificity of trypsin. Based on an alignment of 30 serine protease sequences, it appears that the protein environments of these 21 conserved buried waters are highly conserved. The protein environments of buried waters are comprised primarily of atoms from highly conserved residues or main chain atoms from nonconserved residues. In one instance, the protein environment of a water is conserved even in the presence of an unlikely Pro/Ala substitution. We also note 3 instances in which a histidine side chain substitutes for water, suggesting that the structural role of water at these sites is satisfied by the presence of an alternative hydrogen bonding partner. Buried waters appear to be integral structural components of these proteins and should be incorporated into protein structures predicted on the basis of sequence homology to this family, including the catalytic domains of coagulation proteases.
authors.affiliation: Department of Biochemistry and Molecular Biology, Mayo Clinic and Foundation, Rochester, Minnesota 55905.
authors.forename: U
authors.initials: U
authors.lastname: Sreenivasan
authors.forename: P H
authors.initials: PH
authors.lastname: Axelsen
mesh_terms: D000818:Animals; D053818:Chymases; D002918:Chymotrypsin / Q000737:chemistry; D002919:Chymotrypsinogen / Q000737:chemistry; D003460:Crystallization; D006639:Histidine / Q000737:chemistry; D006801:Humans; D006860:Hydrogen Bonding; D007610:Kallikreins / Q000737:chemistry; D015394:Molecular Structure; D010196:Pancreatic Elastase / Q000737:chemistry; D007703:Peptidyl-Dipeptidase A / Q000737:chemistry; D051381:Rats; D012697:Serine Endopeptidases / Q000737:chemistry*; D013917:Thrombin / Q000737:chemistry; D014357:Trypsin / Q000737:chemistry; D014362:Trypsinogen / Q000737:chemistry; D014867:Water / Q000737:chemistry*; D014961:X-Ray Diffraction
chemical_list: D014867:Water; D006639:Histidine; D014362:Trypsinogen; D002919:Chymotrypsinogen; D007703:Peptidyl-Dipeptidase A; D007610:Kallikreins; D012697:Serine Endopeptidases; D002918:Chymotrypsin; D010196:Pancreatic Elastase; D053818:Chymases; D014357:Trypsin; D013917:Thrombin