Influence of 5-fluorouracil and folinic acid on colonic healing: an experimental study in the rat.
Male Wistar rats were subjected to colonic resection and randomized to one of four groups: control group (intraperitoneal NaCl, intravenous NaCl); 5-fluorouracil (5-FU) group (intraperitoneal 5-FU, intravenous NaCl); folinic acid group (intraperitoneal NaCl, intravenous folinic acid); and 5-FU-folinic acid group (intraperitoneal 5-FU, intravenous folinic acid). Treatment was started immediately after surgery and continued until the animals were killed at 3 or 7 days. Anastomotic complications (abscesses or dehiscence) occurred in four of 33 animals in the control group, 12 of 36 in the 5-FU group, one of 32 in the folinic acid group and nine of 36 in the 5-FU-folinic acid group. Anastomotic and skin breaking strength did not differ between groups on day 3 but by day 7 were significantly reduced in the 5-FU group. In rats given 5-FU-folinic acid, breaking strength was also reduced, but less so than in the 5-FU group. Breaking strength in animals receiving folinic acid was similar to that in the control group. In this model colonic healing was impaired after intraperitoneal 5-FU administration, but when folinic acid was added no further deterioration occurred.
Graf
W
W
Department of Surgery, Akademiska Sjukhuset, Uppsala, Sweden.
Weiber
S
S
Glimelius
B
B
Jiborn
H
H
Påhlman
L
L
Zederfeldt
B
B
D000714:Anastomosis, Surgical; D000818:Animals; D001835:Body Weight; D003106:Colon / Q000187:drug effects / Q000201:enzymology; D005472:Fluorouracil / Q000008:administration & dosage / Q000494:pharmacology*; D006909:Hydroxyproline / Q000378:metabolism; D002955:Leucovorin / Q000008:administration & dosage / Q000494:pharmacology*; D007958:Leukocyte Count; D008297:Male; D009195:Peroxidase / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D012867:Skin / Q000187:drug effects; D013997:Time Factors; D014945:Wound Healing / Q000187:drug effects*
D009195:Peroxidase; D002955:Leucovorin; D006909:Hydroxyproline; D005472:Fluorouracil