title: Abnormal Papanicolaou smears. Comparison of cytology, colposcopy and cervical swab DNA hybridization.
abstract: Cervical swab sampling for DNA hybridization was performed on 89 women attending a colposcopy clinic. In situ hybridization studies for human papillomavirus (HPV) types 6/11, 16/18 and 31/33/35 were performed on the specimens. Biopsy specimens were obtained from 65 patients. The cytologic, colposcopic and histologic results were classified into five groups and assigned a score of 0-5, depending upon the diagnosis. An increasing score correlated positively with the presence of HPV, especially types 16/18 and 31/33/35. A comparison was made of the sensitivity, specificity, and positive and negative predictive values for the standard method of colposcopic evaluation versus the standard method combined with DNA cervical swab sampling. No significant changes in the parameters were found when the DNA cervical swab was added.
authors.affiliation: Division of Gynecology, Hahnemann University Hospital, Philadelphia, PA 19102-1192.
authors.forename: W S
authors.initials: WS
authors.lastname: Powell
authors.forename: H J
authors.initials: HJ
authors.lastname: McKenzie
mesh_terms: D003127:Colposcopy / Q000592:standards*; D003430:Cross-Sectional Studies; D003584:Cytological Techniques / Q000592:standards*; D004247:DNA; D005069:Evaluation Studies as Topic; D005260:Female; D006785:Hospitals, University; D006801:Humans; D009693:Nucleic Acid Hybridization; D010044:Outpatient Clinics, Hospital; D065006:Papanicolaou Test; D027383:Papillomaviridae; D010414:Pennsylvania / Q000453:epidemiology; D012680:Sensitivity and Specificity; D014412:Tumor Virus Infections / Q000175:diagnosis* / Q000453:epidemiology; D014626:Vaginal Smears / Q000379:methods* / Q000592:standards
chemical_list: D004247:DNA