title: Carriers of the mouse rd gene have reduced levels of the beta subunit of the retinal cyclic GMP phosphodiesterase.
abstract: Polyclonal antipeptide antisera have been utilized to quantitate the amount of retinal rod outer segment cGMP phosphodiesterase alpha and beta catalytic subunits present in retinas from C57BL/6J mice which are normal or carriers for the rd gene defect. Results suggest that the quantity of PDE-beta subunit is reduced in carrier mice while PDE-alpha and PDE-gamma are not affected. In 21-day-old mice, the PDE-beta was reduced by about one-half while adult carrier mice had even more reduced levels of PDE-beta. Since PDE alpha was not reduced, this suggests that synthesis of PDE alpha and PDE beta may not be coordinately controlled.
authors.affiliation: Department of Anatomy and Physiology, Kansas State University, Manhattan 66506.
authors.forename: J
authors.initials: J
authors.lastname: Cunnick
authors.forename: D J
authors.initials: DJ
authors.lastname: Takemoto
mesh_terms: D015106:3',5'-Cyclic-GMP Phosphodiesterases / Q000032:analysis / Q000235:genetics* / Q000378:metabolism; D000375:Aging; D000818:Animals; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D005796:Genes; D006580:Genetic Carrier Screening; D006720:Homozygote; D007106:Immune Sera; D046911:Macromolecular Substances; D051379:Mice; D008810:Mice, Inbred C57BL; D012160:Retina / Q000254:growth & development; D012374:Rod Cell Outer Segment / Q000201:enzymology*
chemical_list: D007106:Immune Sera; D046911:Macromolecular Substances; D015106:3',5'-Cyclic-GMP Phosphodiesterases