Agents that stimulate phosphoinositide turnover also elevate cAMP in SK-N-SH human neuroblastoma cells.
Stimulation of m1 and of m3 muscarinic receptors has previously been shown to increase intracellular cAMP levels in a variety of cells. Although the mechanism underlying this response is not fully understood, it has been hypothesized to be secondary to the IP3-mediated rise in intracellular calcium. In order to determine whether other means of elevating intracellular calcium also raise cAMP levels, we stimulated SK-N-SH human neuroblastoma cells with bradykinin or with maitotoxin. Both of these agents stimulated phospholipase C, stimulated inositol phosphate release and elevated cAMP levels, thus demonstrating that this cAMP response is not unique to muscarinic receptor stimulation.
Baumgold
J
J
Department of Radiology, George Washington University Medical Center, Washington, D.C. 20037.
Paek
R
R
Yasumoto
T
T
D001920:Bradykinin / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism; D000242:Cyclic AMP / Q000378:metabolism*; D004789:Enzyme Activation; D006801:Humans; D007295:Inositol Phosphates / Q000378:metabolism; D007424:Intracellular Fluid / Q000378:metabolism; D008387:Marine Toxins / Q000494:pharmacology*; D009447:Neuroblastoma / Q000378:metabolism* / Q000473:pathology; D035964:Oxocins; D010703:Phorbol Esters / Q000494:pharmacology; D010716:Phosphatidylinositols / Q000378:metabolism*; D018002:Receptors, Bradykinin; D011976:Receptors, Muscarinic / Q000187:drug effects; D017981:Receptors, Neurotransmitter / Q000187:drug effects / Q000502:physiology; D013268:Stimulation, Chemical; D014316:Tritium; D014407:Tumor Cells, Cultured; D010738:Type C Phospholipases / Q000378:metabolism; D000095702:Polyether Toxins
D001920:Bradykinin; D002118:Calcium; D000242:Cyclic AMP; D007295:Inositol Phosphates; D008387:Marine Toxins; D035964:Oxocins; D010703:Phorbol Esters; D010716:Phosphatidylinositols; D018002:Receptors, Bradykinin; D011976:Receptors, Muscarinic; D017981:Receptors, Neurotransmitter; D014316:Tritium; D010738:Type C Phospholipases; C020394:maitotoxin; D000095702:Polyether Toxins