[Effect of intracerebroventricular injections of ANG II and ANG II antibody on renal sodium excretion and Na+.K(+)-ATPase in rat].
In anesthetized rats, it was observed that intracerebroventricular (I.C.V.) microinjection of angiotensin II (ANG II) in a dose of 16 pg evoked a significant increase in renal sodium excretion which began within 15 min and lasted for 90 min. The activity of Na+.K(+)-ATPase in renal cortex after I.C.V. microinjection of ANG II (1.51 +/- 0.26 mumol Pi/mg Pro.h) was inhibited as compared with that of the control injecting of artificial cerebrospinal fluid (2.66 +/- 0.28 mumol Pi/mg Pro.h, P less than 0.01). There was no change in mean arterial pressure. Within 15 min after I.C.V. administration of ANG II antibody, however, and antinatriuretic period of 135 min and a higher activity of Na+.K(+)-ATPase in renal cortex (3.61 +/- 0.34 mumol Pi/mg Pro.h, P less than 0.05 compared with control) were observed. There was no natriuresis in the animals microinjected with ANG II either into femoral vein or into spinal subarachnoid space. The result of the present investigation suggests that brain endogenous ANG II may possess some natriuretic activity possibly through inhibiting renal Na+.K(+)-ATPase activity.
Li
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Department of Physiology, Zun Yi Medical College.
Gao
Y
Y
Yu
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AQ
D000804:Angiotensin II / Q000276:immunology / Q000494:pharmacology*; D000818:Animals; D000906:Antibodies / Q000008:administration & dosage; D005260:Female; D007276:Injections, Intraventricular; D007668:Kidney / Q000502:physiology*; D007672:Kidney Cortex / Q000201:enzymology; D008297:Male; D009318:Natriuresis / Q000187:drug effects*; D051381:Rats; D011919:Rats, Inbred Strains; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*
D000906:Antibodies; D000804:Angiotensin II; D000254:Sodium-Potassium-Exchanging ATPase