title: Spectroscopic characterization of the alternate form of S-methylcoenzyme M reductase from Methanobacterium thermoautotrophicum (strain delta H).
abstract: Two forms (MR1 and MR2) of S-methylcoenzyme M reductase were purified from Methanobacterium thermoautotrophicum (strain delta H) as recently described (Rospert, S., Linder, D., Ellerman, J. and Thauer, R.K. (1990) Eur. J. Biochem. 194, 871-877). MR2 was at least 50-fold more active than MR1, independent of assay conditions. The two forms are spectroscopically similar, but not identical, by UV-visible, magnetic circular dichroism and resonance Raman spectroscopies. MR2 exhibited an EPR signal corresponding to 20% of the enzyme-bound nickel. Strong EPR signals similar to those previously assigned to Ni(I)F430 bound to methylreductase in Methanobacterium thermoautotrophicum (strain Marburg) (Albracht, S.P.J., Ankel-Fuchs, D., Bocher, R., Ellerman, J., Moll, J., Van der Zwann, J.W. and Thauer, R.K. (1988) Biochim. Biophys. Acta 955, 86-102) were observed in MR2-rich, log-phase, as well as in MR1-rich, slow-growing bacteria. Log-phase cells had dramatically different EPR spectra depending on whether they were removed from the fermenter (under gas flow) before or after cooling to 10 degrees C. EPR spectra of slow-growing cells were insensitive to harvesting conditions. The possible biological significance of the alternate form of methylreductase is discussed.
authors.affiliation: Department of Chemistry, University of Georgia, Athens 30602.
authors.forename: M C
authors.initials: MC
authors.lastname: Brenner
authors.forename: L
authors.initials: L
authors.lastname: Ma
authors.forename: M K
authors.initials: MK
authors.lastname: Johnson
authors.forename: R A
authors.initials: RA
authors.lastname: Scott
mesh_terms: D002942:Circular Dichroism; D004578:Electron Spin Resonance Spectroscopy; D017014:Methanobacterium / Q000201:enzymology* / Q000254:growth & development; D008970:Molecular Weight; D010088:Oxidoreductases / Q000737:chemistry* / Q000378:metabolism; D013057:Spectrum Analysis
chemical_list: D010088:Oxidoreductases; C021572:methyl coenzyme M reductase