title: Primary structure, chromosomal localization, and functional expression of a voltage-gated sodium channel from human brain.
abstract: A cDNA library derived from human cerebral cortex was screened for the presence of sodium channel alpha subunit-specific clones. Ligation of three overlapping clones generated a full-length cDNA clone, HBA, that provided the complete nucleotide sequence coding for a protein of 2005 amino acids. The predicted structure suggests four homologous repeats and exhibits greatest homology and structural similarity to the rat brain sodium channel II. A second cDNA clone, HBB, that encodes a different subtype of sodium channel was isolated. Hybridization of DNA fragments from the 3' untranslated region of HBA and PCR with primers derived from HBB with human-hamster somatic cell hybrids localized these clones to human chromosome 2. In situ hybridization to human metaphase chromosomes mapped the structural genes for both HBA and HBB sodium channels to chromosome 2q23-24.3. The sodium channel HBA gene product was expressed by transfection in CHO cells. Expressed HBA currents were voltage-dependent, sodium-selective, and tetrodotoxin-sensitive and, thus, exhibit the biophysical and pharmacological properties characteristic of sodium channels.
authors.affiliation: Department of Biology, University of California, San Diego, La Jolla 92093.
authors.forename: C M
authors.initials: CM
authors.lastname: Ahmed
authors.forename: D H
authors.initials: DH
authors.lastname: Ware
authors.forename: S C
authors.initials: SC
authors.lastname: Lee
authors.forename: C D
authors.initials: CD
authors.lastname: Patten
authors.forename: A V
authors.initials: AV
authors.lastname: Ferrer-Montiel
authors.forename: A F
authors.initials: AF
authors.lastname: Schinder
authors.forename: J D
authors.initials: JD
authors.lastname: McPherson
authors.forename: C B
authors.initials: CB
authors.lastname: Wagner-McPherson
authors.forename: J J
authors.initials: JJ
authors.lastname: Wasmuth
authors.forename: G A
authors.initials: GA
authors.lastname: Evans
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D016466:CHO Cells; D002889:Chromosomes, Human, Pair 2; D003001:Cloning, Molecular; D006224:Cricetinae; D004247:DNA / Q000235:genetics; D006801:Humans; D015640:Ion Channel Gating; D008564:Membrane Potentials; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D015183:Restriction Mapping; D016415:Sequence Alignment; D015222:Sodium Channels / Q000235:genetics* / Q000502:physiology; D014162:Transfection
chemical_list: D009838:Oligodeoxyribonucleotides; D015222:Sodium Channels; D004247:DNA