Polyclonal B-lymphocyte activation of sheep by Mycobacterium phlei against sheep pox virus.
A trypsinized preparation of Mycobacterium phlei, non specific stimulator of immunity (NSI), and Sheep Pox Virus (SPV) were inoculated in different groups of sheep to activate B-lymphocytes and induce SPV neutralizing substance(s). NSI sensitized sheep B-lymphocytes in the presence of NSI or lymphokine elaborated SPV neutralizing substance(s). The SPV sensitized B-lymphocytes also mediated such neutralizing substance(s). Healthy control sheep B-lymphocytes failed to show any appreciable amount of viral neutralizing substance. However, a significant virus neutralizing substance(s) was detected when healthy sheep B-lymphocytes were cultured in presence of NSI antigen along with lymphokines.
Gangopadhyay
N N
NN
Immunostimulation Laboratory, Indian Veterinary Research Institute, Izatnagar.
Mallick
B B
BB
D000818:Animals; D015214:Antigens, Differentiation, Myelomonocytic / Q000276:immunology; D000951:Antigens, Neoplasm; D001402:B-Lymphocytes; D015815:Cell Adhesion Molecules; D005260:Female; D007111:Immunity, Cellular; D008213:Lymphocyte Activation; D008297:Male; D008562:Membrane Glycoproteins / Q000276:immunology; D009168:Mycobacterium phlei / Q000276:immunology*; D011212:Poxviridae / Q000276:immunology; D011213:Poxviridae Infections / Q000276:immunology* / Q000517:prevention & control; D012756:Sheep; D014611:Vaccination
D015214:Antigens, Differentiation, Myelomonocytic; D000951:Antigens, Neoplasm; D015815:Cell Adhesion Molecules; D008562:Membrane Glycoproteins