Fluorescent location of brain tumour cells in wax embedded sections.
Tumour cells and certain normal cells in wax embedded brain sections possess active isoenzymic forms of a cell surface protease. The normal isoenzymic forms are recognised by protease inhibitors present in normal serum; the tumour isoenzyme remains active after exposure to serum. Fluorescent location of cells possessing active isoenzyme after serum treatment reveals the tumour cells in these sections.
Steven
F S
FS
Department of Biochemistry and Molecular Biology, School of Biological Sciences, University of Manchester, U.K.
Reid
H
H
D001254:Astrocytoma / Q000201:enzymology / Q000473:pathology*; D001932:Brain Neoplasms / Q000201:enzymology / Q000473:pathology*; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism*; D003116:Color; D010450:Endopeptidases; D005453:Fluorescence; D005909:Glioblastoma / Q000201:enzymology / Q000473:pathology*; D006801:Humans; D008856:Microscopy, Fluorescence; D016610:Tissue Embedding; D014885:Waxes
D014885:Waxes; D002265:Carboxylic Ester Hydrolases; C037288:guanidinobenzoate esterase; D010450:Endopeptidases