The m gamma delta-1 element, a small gamma delta (Tn1000) derivative useful for plasmid mutagenesis, allele replacement and DNA sequencing.
Transposon gamma delta (Tn1000), a 6-kb member of the Tn3 family, is widely used for plasmid mutagenesis. A 1.8-kb derivative of gamma delta was constructed that contains the kan gene from Tn5 and the resolution (res) site from gamma delta cloned between 40-bp inverted repeats of gamma delta's delta (delta) end. This element, named m gamma delta-1, lacks the genes encoding transposase and resolvase, and therefore depends on its host to supply transposition and resolution functions. Thus, in strains lacking gamma delta, m gamma delta-1 will not transpose. The m gamma delta-1 element is shown to be useful for mutagenesis of plasmids, DNA sequencing, and allele replacement (in Streptomyces avermitilis).
Berg
C M
CM
Department of Molecular and Cell Biology, University of Connecticut, Storrs 06269-2131.
Vartak
N B
NB
Wang
G
G
Xu
X
X
Liu
L
L
MacNeil
D J
DJ
Gewain
K M
KM
Wiater
L A
LA
Berg
D E
DE
D000483:Alleles; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000235:genetics*; D005838:Genotype; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D009713:Nucleotidyltransferases / Q000235:genetics*; D009838:Oligodeoxyribonucleotides; D010957:Plasmids; D015183:Restriction Mapping; D013302:Streptomyces / Q000235:genetics*; D019895:Transposases
D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D009838:Oligodeoxyribonucleotides; D009713:Nucleotidyltransferases; D019895:Transposases