[The physicochemical and biological properties of the bovine hemoglobin polymer].
A study was made of bovine hemoglobin polymer obtained by a new method: intraerythrocytic polymerization of hemoglobin followed by isolation of hemoglobin polymer. The polymer obtained had weighted mean and numerical average molecular mass of 388 kD and 134 kD, respectively, P 32 mm Hg, arteriovenous difference 5.1 vol%, Hill's coefficient 2.14, oxygen output rate constant 55 sec, and it retained its activity during long-term storage. Its solution in a concentration of 9-10 g/dl has a viscosity of 2.34, and an oncotic pressure of 240 mm water. Bovine hemoglobin polymer is nontoxic and capable of long-term circulation in the blood. Its safety and clinical effectiveness should be studied in an extended biological experiment.
Smirnov
I V
IV
Khachatur'ian
A A
AA
Fetisova
L V
LV
Ivanov
Iu G
IuG
Groznaia
T G
TG
Smirnova
I L
IL
Vorob'eva
T M
TM
Akimova
T A
TA
Iarochkin
V S
VS
Rozenberg
G Ia
GIa
D000818:Animals; D001802:Blood Substitutes / Q000737:chemistry* / Q000493:pharmacokinetics / Q000633:toxicity; D002417:Cattle; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004355:Drug Stability; D005260:Female; D006207:Half-Life; D006454:Hemoglobins; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D051379:Mice; D008970:Molecular Weight; D009997:Osmotic Pressure; D010084:Oxidation-Reduction; D010108:Oxyhemoglobins / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
D001802:Blood Substitutes; D006454:Hemoglobins; D010108:Oxyhemoglobins; C079129:hemoglobin polymer
Izuchenie fiziko-khimicheskikh i riada biologicheskikh svoĭstv polimera gemoglobina byka.