title: Expression of recombinant myeloperoxidase using a baculovirus expression system.
abstract: Myeloperoxidase (MPO) is a glycosylated heme-containing enzyme present in the azurophilic granules of normal human polymorphonuclear neutrophils. This enzyme plays a major role in the microbicidal activity of the host defense system by catalyzing the formation of the potent oxidant, hypochlorous acid. Although the amino acid sequence of MPO has been deduced from the cDNA, the structural basis for the observed heterogeneity of this enzyme is not known. Furthermore, the nature of the prosthetic group and its mode of linkage to the apoprotein has not been determined. To address questions regarding the structural features of MPO, which arise during the complex posttranslational processing of this enzyme, we utilized a baculovirus system to express MPO in Sf9 insect cells. Two glycosylated, single-chain precursor species of MPO were observed: an 84 kDa species that was secreted and a 74 kDa species that was cell-associated. This is the first report of an expression system in which a cell-associated MPO precursor undergoes posttranslational proteolytic processing.
authors.affiliation: Department of Biochemistry, Emory University School of Medicine, Atlanta, GA 30322-3050.
authors.forename: K L
authors.initials: KL
authors.lastname: Taylor
authors.forename: D J
authors.initials: DJ
authors.lastname: Uhlinger
authors.forename: J M
authors.initials: JM
authors.lastname: Kinkade
mesh_terms: D000818:Animals; D016367:Baculoviridae; D015153:Blotting, Western; D002478:Cells, Cultured; D003001:Cloning, Molecular; D005822:Genetic Vectors; D006031:Glycosylation; D006801:Humans; D066298:In Vitro Techniques; D007313:Insecta; D008970:Molecular Weight; D009195:Peroxidase / Q000235:genetics* / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D011994:Recombinant Proteins / Q000276:immunology / Q000378:metabolism
chemical_list: D011994:Recombinant Proteins; D009195:Peroxidase