Sequence analysis of the large (L) protein of simian virus 5.
The complete nucleotide sequence of the large (L) protein gene of simian virus 5 (SV5) was determined from cDNA of the genomic RNA and mRNA, and found to be 6804 bases in length, exclusive of a poly(A) tract. The sequence contained an open reading frame of 6765 nucleotides encoding 2255 amino acids. Results of dot matrix comparisons of the L protein of SV5 with those of human parainfluenza type 3 virus and Sendai virus indicated that there are five conserved domains, and that each domain contains characteristic sequence(s). The L protein of SV5 was detected in purified virions using antiserum directed against an oligopeptide corresponding to the N-terminal region.
Higuchi
Y
Y
Department of Microbiology, Mie University School of Medicine, Japan.
Miyahara
Y
Y
Kawano
M
M
Tsurudome
M
M
Matsumura
H
H
Kusagawa
S
S
Komada
H
H
Nishio
M
M
Ito
Y
Y
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004277:DNA, Single-Stranded / Q000235:genetics; D008969:Molecular Sequence Data; D010222:Parainfluenza Virus 1, Human / Q000235:genetics; D010224:Parainfluenza Virus 3, Human / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics; D015301:Retroviruses, Simian / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D014764:Viral Proteins / Q000032:analysis / Q000235:genetics*; D014771:Virion / Q000737:chemistry
D004277:DNA, Single-Stranded; D012333:RNA, Messenger; D012367:RNA, Viral; D014764:Viral Proteins; C075512:L protein, Simian parainfluenza virus 5