Equivalence of a high-performance liquid chromatographic assay and a bioassay of azithromycin in human serum samples.
Two sensitive methods for the determination of the azalide antibiotic azithromycin in human serum were compared. High-performance liquid chromatography (HPLC) and a microbiological assay were simultaneously applied to 768 serum samples obtained in a clinical study. There was excellent agreement between the azithromycin concentrations measured by HPLC and by the bioassay. The correlation coefficient for the two methods was r2 = 0.96. The precision and the sensitivity of the methods were found to be very similar.
Riedel
K D
KD
Department of Research and Development, Pfizer/Mack, Illertissen, Germany.
Wildfeuer
A
A
Laufen
H
H
Zimmermann
T
T
D017963:Azithromycin; D001681:Biological Assay; D002851:Chromatography, High Pressure Liquid; D004917:Erythromycin / Q000031:analogs & derivatives* / Q000097:blood / Q000493:pharmacokinetics; D006801:Humans
D004917:Erythromycin; D017963:Azithromycin