Depression of early protection against influenza virus infection by cyclophosphamide and its restoration by protein-bound polysaccharide.
Relationship between depression of early protection against influenza virus infection and the decrease in the number of peripheral polymorphonuclear leukocytes in cyclophosphamide-treated mice was investigated using protein-bound polysaccharide (PSK), which had been shown to exert a potent restorative effect on leukocytopenia in immunocompromised hosts. Following intranasal inoculation with influenza virus (1.5 x 10(3) PFU) into untreated mice, the pulmonary virus titer progressively increased during 3 days and decreased gradually from the day 7 after infection. The treatment of mice with cyclophosphamide (150 mg/kg) 2 days before infection markedly enhanced the pulmonary virus multiplication from the early phase of infection, and the higher virus titer was maintained thereafter. When mice were given cyclophosphamide after PSK-treatment, virus titers from the early to late phases of infection were lower than those in untreated mice.
Tsuru
S
S
Department of Microbiology, National Defense Medical College, Saitama, Japan.
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D003520:Cyclophosphamide / Q000494:pharmacology*; D003602:Cytotoxicity, Immunologic; D005260:Female; D009980:Influenza A virus / Q000254:growth & development / Q000276:immunology*; D008168:Lung / Q000382:microbiology; D051379:Mice; D008807:Mice, Inbred BALB C; D009504:Neutrophils / Q000276:immunology; D009976:Orthomyxoviridae Infections / Q000276:immunology* / Q000382:microbiology; D011509:Proteoglycans / Q000494:pharmacology*; D011817:Rabbits
D000276:Adjuvants, Immunologic; D000914:Antibodies, Viral; D011509:Proteoglycans; C010770:polysaccharide-K; D003520:Cyclophosphamide