In vitro interactions of anionic and cationic surfactants with salivary fractions on well-defined solid surfaces.
Ellipsometry was used to study the interaction of one anionic (SDS) and one cationic (CTAB) surfactant with films adsorbed from six different salivary fractions obtained after fractionation of whole unstimulated saliva on a Superdex 200 Hiload gel filtration column. Experiments were performed on both hydrophilic silica and hydrophobic methylated silica surfaces. The results of this study indicate that the adhesive and cohesive properties of the films adsorbed from the individual fractions were strongly dependent on the surface characteristics of the substrates and that the outcome of protein/surfactant interactions was dependent on factors such as protein composition, surfactant charge, and substrate characteristics. These interactions probably involve replacement of the adsorbed proteins by surfactants or protein/surfactant complex formation. The anionic surfactant seemed to be more efficient in removing adsorbed salivary proteins than the cationic one.
Vassilakos
N
N
Department of Prosthetic Dentistry, Dental School, University of Lund, Sweden.
Arnebrant
T
T
Rundegren
J
J
Glantz
P O
PO
D000268:Adhesiveness; D000327:Adsorption; D000838:Anions / Q000737:chemistry; D002412:Cations / Q000737:chemistry; D000077286:Cetrimonium; D002593:Cetrimonium Compounds / Q000737:chemistry; D006801:Humans; D008970:Molecular Weight; D012471:Salivary Proteins and Peptides / Q000737:chemistry*; D012825:Silicon / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry; D013499:Surface Properties; D013501:Surface-Active Agents / Q000737:chemistry*; D014867:Water
D000838:Anions; D002412:Cations; D002593:Cetrimonium Compounds; D012471:Salivary Proteins and Peptides; D013501:Surface-Active Agents; D014867:Water; D012967:Sodium Dodecyl Sulfate; D012822:Silicon Dioxide; D012825:Silicon; D000077286:Cetrimonium