Subcellular distribution of ubiquitin-protein conjugates in the hippocampus following transient ischemia.
Ubiquitin-protein conjugates in the hippocampus were analyzed by immunoblotting with a monoclonal anti-ubiquitin antibody. In the CA1 region, Triton X-100 insoluble ubiquitin-protein conjugates increased after 24 hr following 20 min of ischemia. When the total hippocampi were fractionated subcellularly, ubiquitin-protein conjugates increased in the particulate, especially in the mitochondrial fraction. The ubiquitin-protein conjugates were solubilized by SDS, or were partially solubilized by urea. The results indicate that insoluble ubiquitin-protein conjugates increase after ischemia.
Hayashi
T
T
Department of Biochemistry, Jikei University School of Medicine, Tokyo, Japan.
Takada
K
K
Matsuda
M
M
D000818:Animals; D000911:Antibodies, Monoclonal; D015153:Blotting, Western; D016923:Cell Death / Q000502:physiology; D006624:Hippocampus / Q000378:metabolism*; D066298:In Vitro Techniques; D002546:Ischemic Attack, Transient / Q000378:metabolism*; D008297:Male; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013347:Subcellular Fractions / Q000378:metabolism*; D014452:Ubiquitins / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D009419:Nerve Tissue Proteins; D014452:Ubiquitins