Evaluation of two ELISA kits for the detection of Aujeszky's disease antibodies in pigs.
This work describes the evaluation of two commercial ELISA kits for the detection of gI antibodies against Aujeszky's disease. A collection of experimental sera from infected pigs, field sample sera, and sera from pigs vaccinated with seven different modified gI-negative commercial vaccines were used to evaluate each test. Both ELISA kits showed good reproducibility, and specificity, but differences could be appreciated in sensitivity when sera obtained at early stages of infection was analysed. These results also indicated that both kits could be used in conjunction with the seven vaccines evaluated in this study.
Arias
M
M
Departamento de Sanidad Animal, Instituto Nacional de Investigaciones Agrarias, Madrid, Spain.
Moyano
M
M
Escribano
J M
JM
Sánchez-Vizcaino
J M
JM
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary*; D005069:Evaluation Studies as Topic; D005189:False Positive Reactions; D011558:Herpesvirus 1, Suid / Q000276:immunology*; D011557:Pseudorabies / Q000276:immunology*; D011897:Random Allocation; D011933:Reagent Kits, Diagnostic / Q000662:veterinary; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013552:Swine; D013553:Swine Diseases / Q000276:immunology*; D014611:Vaccination / Q000662:veterinary; D014759:Viral Envelope Proteins / Q000276:immunology; D014765:Viral Vaccines / Q000276:immunology
D000914:Antibodies, Viral; D011933:Reagent Kits, Diagnostic; D014759:Viral Envelope Proteins; D014765:Viral Vaccines; C042966:pseudorabies virus glycoproteins