Complete 1H- and 13C-resonance assignments for D-mannooligosaccharides of the beta-D-(1-->2)-linked series released from the phosphopeptidomannan of Candida albicans VW.32 (serotype A).
D-Mannooligosaccharides (dp 1 to > 17) were released by mild acid hydrolysis from the phosphopeptidomannan of a Candida albicans strain of A serotype (VW.32). Among these, mannooligosaccharides ranging from bi- to hepta-ose, which were obtained in appreciable amounts, were structurally investigated and found to belong to the beta-D-(1-->2)-linked series. The occurrence of such compounds has already been reported in other Candida albicans strains. The complete 1H- and 13C-resonance assignments for manno-tri- to manno-hepta-ose are reported and general rules applicable for the 1NMR spectrum analysis of linear mannooligosaccharide of the general structure, beta-D-Man p-(1-->2)-[beta-D-Man p-(1-->2)]n-beta-D-Man p are proposed.
Faille
C
C
Unité 42, Institut National de la Santé et de la Recherche Médicale, Villeneuve d'Ascq, France.
Wieruszeski
J M
JM
Michalski
J C
JC
Poulain
D
D
Strecker
G
G
D000143:Acids; D002176:Candida albicans / Q000378:metabolism*; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002247:Carbon Isotopes; D006868:Hydrolysis; D009682:Magnetic Resonance Spectroscopy; D008351:Mannans / Q000378:metabolism*; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry*; D011522:Protons; D013053:Spectrophotometry / Q000379:methods
D000143:Acids; D002247:Carbon Isotopes; D008351:Mannans; D009844:Oligosaccharides; D011522:Protons