The procoagulant response of cytomegalovirus infected endothelial cells.
The report describes the effect of an in vitro infection of human umbilical vein endothelial cells with human Cytomegalovirus (CMV). The parameters studied are cellular procoagulant activity, secretion of plasminogen activator inhibitor (PAI-1) and urokinase-type plasminogen activator (u-PA), activation and internalization of factor X and Merocyanine 540 staining. The infection does not result in an increase in PAI-1 and u-PA secretion, but it brings about a procoagulant response, which is relatively rapid compared to the tissue factor mediated response induced by inflammatory mediators. The time course and the coagulation factor dependency suggest a facilitated interaction of coagulation factors on the surface of infected cells. Chromogenic activity measurements after the addition of purified factor X and electron microscopic examination of the cells after addition of colloidal gold-factor X conjugates both point to an internalization of factor X and/or Xa after interaction with the endothelial cell surface. Merocyanine 540 staining suggests that CMV infection leads to membrane perturbations.
van Dam-Mieras
M C
MC
Open University of The Netherlands, Dept. Natural Sciences, Heerlen.
Muller
A D
AD
van Hinsbergh
V W
VW
Mullers
W J
WJ
Bomans
P H
PH
Bruggeman
C A
CA
D001777:Blood Coagulation / Q000502:physiology*; D002478:Cells, Cultured; D002863:Chromogenic Compounds; D003586:Cytomegalovirus Infections / Q000503:physiopathology*; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D005170:Factor X / Q000378:metabolism; D015951:Factor Xa / Q000378:metabolism; D006801:Humans; D017395:Plasminogen Activator Inhibitor 1 / Q000032:analysis / Q000378:metabolism; D013917:Thrombin / Q000494:pharmacology; D014568:Urokinase-Type Plasminogen Activator / Q000276:immunology / Q000378:metabolism
D002863:Chromogenic Compounds; D017395:Plasminogen Activator Inhibitor 1; D005170:Factor X; D013917:Thrombin; D015951:Factor Xa; D014568:Urokinase-Type Plasminogen Activator