Molecular cloning and mapping of phenol degradation genes from Bacillus stearothermophilus FDTP-3 and their expression in Escherichia coli.
Two genes of the meta pathway of phenol degradation were cloned from a phenol-utilizing strain of Bacillus stearothermophilus and were mapped by subcloning and by use of a Tn5 insertion mutation. They code for phenol hydroxylase and catechol 2,3-dioxygenase, respectively. The gene encoding catechol 2,3-dioxygenase, which is more thermostable than catechol 2,3-dioxygenase encoded by the other gene, shares rather limited homology with that from Pseudomonas putida.
Dong
F M
FM
Institute of Genetics, Fudan University, Shanghai, People's Republic of China.
Wang
L L
LL
Wang
C M
CM
Cheng
J P
JP
He
Z Q
ZQ
Sheng
Z J
ZJ
Shen
R Q
RQ
D050557:Catechol 2,3-Dioxygenase; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004251:DNA Transposable Elements; D049308:Dioxygenases; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D005798:Genes, Bacterial; D001411:Geobacillus stearothermophilus / Q000235:genetics* / Q000378:metabolism*; D006899:Mixed Function Oxygenases / Q000235:genetics; D016254:Mutagenesis, Insertional; D010105:Oxygenases / Q000235:genetics; D019800:Phenol; D010636:Phenols / Q000378:metabolism*
D004251:DNA Transposable Elements; D010636:Phenols; D019800:Phenol; D006899:Mixed Function Oxygenases; D010105:Oxygenases; D049308:Dioxygenases; D050557:Catechol 2,3-Dioxygenase; C022336:phenol 2-monooxygenase