Hybridization of transposon Tn501 for detection of mercury resistance sequences in a marine environment.
Total genomic DNA isolated by concentrating seawater and mercury-resistant bacteria were hybridized with a mer probe to detect the presence of homologous DNA sequences in marine coastal waters of the Bay of Bengal, India. Coastal water extracts induced with mercury hybridized with the mer operon of transposon Tn501. Most of the mercury-resistant bacteria that volatilized mercury also contained homologous DNA sequences to the mer probe.
Prabu
S K
SK
Centre for Advanced Study in Botany, University of Madras, India.
Mahadevan
A
A
D001419:Bacteria / Q000235:genetics* / Q000378:metabolism; D004251:DNA Transposable Elements / Q000502:physiology*; D004269:DNA, Bacterial / Q000235:genetics; D004352:Drug Resistance, Microbial; D066298:In Vitro Techniques; D007194:India; D008628:Mercury / Q000378:metabolism*; D009693:Nucleic Acid Hybridization / Q000502:physiology*; D009876:Operon / Q000235:genetics; D012623:Seawater / Q000032:analysis*
D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D008628:Mercury