Site-specific incorporation of [125I]iododeoxyuridine into DNA.
A procedure for the incorporation of [125I]IdU into specific sites in DNA is described. The approach depends upon attachment of radioiododeoxyuridine to a controlled pore glass support which is then used for automated synthesis of an oligomer. The resulting oligomer, containing a terminal 3'[125I]iododeoxyuridine, is used as a primer during DNA synthesis catalyzed by the Taq polymerase employing thermal cycling. The product formed includes the radioiodonucleotide at a single internal site determined by the length of the oligomer.
Scherberg
N
N
University of Chicago Hospitals and Clinics, IL.
Bloch
I
I
Gardner
P
P
D001483:Base Sequence; D001665:Binding Sites; D004247:DNA / Q000737:chemistry*; D007065:Idoxuridine / Q000737:chemistry*; D007457:Iodine Radioisotopes; D008969:Molecular Sequence Data
D007457:Iodine Radioisotopes; D004247:DNA; D007065:Idoxuridine