Planarian mitochondria. I. Heterogeneity of cytochrome c oxidase subunit I gene sequences in the freshwater planarian, Dugesia japonica.
We have detected sequence heterogeneity in the cytochrome c oxidase subunit I (COI) gene of freshwater planarian, Dugesia japonica, collected in one locality. A part of the COI gene was amplified via the polymerase chain reaction (PCR) using template DNA prepared from a mixture of 500 individuals or from each of 18 individuals. Analyses of DNA sequences by standard strategies for cloning and sequencing or by direct sequencing clearly show that (1) considerable sequence heterogeneity exists in DNA prepared from the mixed individuals, (2) 11 individuals have almost identical sequences (type A), and (3) 7 individuals have sequences different from one another (Seq-D1 to Seq-D7; collectively called type D). Each of the Seq-D1-D7 sequences except for Seq-D5 shows some heterogeneity even in a single individual (heteroplasmy). A possible cause of the sequence heterogeneities is discussed.
Bessho
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Y
Department of Biology, School of Science, Nagoya University, Japan.
Ohama
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T
Osawa
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S
D000818:Animals; D001483:Base Sequence; D004272:DNA, Mitochondrial / Q000235:genetics*; D003576:Electron Transport Complex IV / Q000235:genetics*; D008969:Molecular Sequence Data; D005810:Multigene Family; D010932:Planarians / Q000201:enzymology / Q000235:genetics*; D011003:Ploidies; D012100:Reproduction, Asexual / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid
D004272:DNA, Mitochondrial; D003576:Electron Transport Complex IV