Mapping the clock rhythm mutation to the period locus of Drosophila melanogaster by germline transformation.
The Clock (Clk) mutation shortens circadian rhythms of locomotor activity and eclosion from ca. 24 h to 22.5-23 h. Clk was previously mapped, by meiotic recombination, very close to the period(per) locus on the X chromosome. To determine whether Clk is a mutation within the per gene or if the former is separate from the latter, two overlapping genomic fragments were cloned from Clk flies to produce a per-containing 13.2 kb construct, per01 flies (which by themselves are arrhythmic)--when transformed with this construct--expressed short-period rhythms. This indicates that the Clk mutation is contained within this 13.2 kb region and is almost certainly a new "fast-clock" allele of per.
Dushay
M S
MS
Department of Biology, Brandeis University, Waltham, Massachusetts 02254.
Rosbash
M
M
Hall
J C
JC
D000483:Alleles; D000818:Animals; D002874:Chromosome Mapping / Q000379:methods*; D002940:Circadian Rhythm / Q000235:genetics*; D004251:DNA Transposable Elements / Q000235:genetics; D004331:Drosophila melanogaster / Q000235:genetics*; D005815:Genetic Code / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics; D016678:Genome; D005854:Germ Cells / Q000502:physiology*; D008297:Male; D009043:Motor Activity / Q000235:genetics; D009154:Mutation / Q000235:genetics; D014158:Transcription, Genetic / Q000235:genetics*; D014170:Transformation, Genetic / Q000235:genetics*; D014960:X Chromosome
D004251:DNA Transposable Elements