title: Thermally induced chain exchange of frog alpha beta-tropomyosin.
abstract: The thermally induced unfolding of the alpha-helix of Rana esculenta alpha alpha, alpha beta and beta beta tropomyosin and two tryptic fragments approximately corresponding to the N- and C-terminal halves of alpha beta have been investigated by use of optical rotation, circular dichroism and UV difference spectroscopy. Reversible unfolding transitions of alpha alpha and beta beta occur around 49 degrees C and 32 degrees C, respectively. The helix unfolding of alpha beta shows two major transitions at 36 degrees C and 48 degrees C, with only the latter being reversible. The major unfolding transitions of each of the N- and C-terminal alpha beta peptides roughly correspond to the low and high temperature transitions of intact alpha beta, respectively. This suggests that the unfolding of alpha beta could be due to unfolding of two independent domains in alpha beta. UV difference data, crosslinking and chromatography results show, however, that the unfolding of alpha beta at 36 degrees C is due to chain exchange with the formation of alpha alpha homodimers and largely unfolded beta monomers, and that the transition at 48 degrees C is due to unfolding of alpha alpha dimers.
authors.affiliation: Department of Chemistry, Roskilde University, Denmark.
authors.forename: S
authors.initials: S
authors.lastname: Hvidt
authors.forename: S S
authors.initials: SS
authors.lastname: Lehrer
mesh_terms: D000818:Animals; D002845:Chromatography; D002942:Circular Dichroism; D017886:Durapatite; D006882:Hydroxyapatites; D046911:Macromolecular Substances; D009132:Muscles; D009904:Optical Rotation; D010446:Peptide Fragments / Q000737:chemistry; D011489:Protein Denaturation; D017510:Protein Folding; D017433:Protein Structure, Secondary; D011893:Rana esculenta; D013816:Thermodynamics; D014335:Tropomyosin / Q000737:chemistry* / Q000302:isolation & purification; D014357:Trypsin; D014443:Tyrosine
chemical_list: D006882:Hydroxyapatites; D046911:Macromolecular Substances; D010446:Peptide Fragments; D014335:Tropomyosin; D014443:Tyrosine; D017886:Durapatite; D014357:Trypsin