An application of 3D-QSAR to the analysis of the sequence specificity of DNA alkylation by uracil mustard.
The sequence specificity of DNA alkylation by uracil mustard was examined using a novel three-dimensional QSAR method known as HASL, or the hypothetical active site lattice. The structures of a variety of 4-mer sequences obtained from pBR322 and SV40 were related to their degree of guanine-N7 alkylation by uracil mustard. The resulting correlations were found to point to a significant contribution from bases on the 3' side of the target guanine nucleotide. The HASL models derived from the analysis of 52 guanine-containing 4-mer sequences were used to highlight those atomic features in the favored TGCC sequence that were found most important in determining specificity. It was found that the NH2-O systems present in the two GC base pairs on the 3' side of the target guanine were significantly correlated to the degree of alkylation by uracil mustard. This finding is consistent with a prealkylation binding event occurring between these sites along the major groove and the uracil mustard O2/O4 system.
Doweyko
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AM
CIBA-GEIGY Corporation, Environmental Health Center, Farmington, Connecticut 06032.
Mattes
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WB
D000478:Alkylation; D004247:DNA / Q000737:chemistry*; D004269:DNA, Bacterial / Q000737:chemistry; D004279:DNA, Viral / Q000737:chemistry; D006150:Guanine Nucleotides / Q000737:chemistry*; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D010957:Plasmids / Q000737:chemistry; D012680:Sensitivity and Specificity; D013539:Simian virus 40 / Q000737:chemistry; D013329:Structure-Activity Relationship; D014499:Uracil Mustard / Q000737:chemistry*
D004269:DNA, Bacterial; D004279:DNA, Viral; D006150:Guanine Nucleotides; D004247:DNA; D014499:Uracil Mustard