title: [Plasmid localization and cloning of restriction modification genes from Citrobacter freundii 4111 strain].
abstract: Over 60 producing strains of restriction endonucleases type II have been found among 500 different strains, mostly Enterobacteriaceae. The strain Citrobacter freundii 4111 produces restriction endonuclease CfrBI, a new isoschisomer of StyI. The genes of the restriction-modification system CfrBI were located on the multicopy plasmid pZE8 containing the Co1E1-type replicon and cloned to E. coli K802. The deletion variant of 3.2-kb pZE8 which contains intact restriction-modification and a DNA fragment responsible for autonomous plasmid replication was selected among the recombinant plasmids. The strain with higher R. CfrBI production (at least 10,000,000 U/g cells, which is 500-fold higher than the wild strain) was constructed.
authors.forename: A N
authors.initials: AN
authors.lastname: Kravets
authors.forename: A S
authors.initials: AS
authors.lastname: Solonin
authors.forename: M V
authors.initials: MV
authors.lastname: Zakharova
authors.forename: Z E
authors.initials: ZE
authors.lastname: Tarutina
mesh_terms: D016971:Citrobacter freundii / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes / Q000096:biosynthesis / Q000235:genetics*; D005798:Genes, Bacterial; D010957:Plasmids; D011995:Recombination, Genetic; D013045:Species Specificity
chemical_list: D004262:DNA Restriction Enzymes
vernacular_title: Plazmidnaia lokalizatsiia i klonirovanie genov sistemy restriktsii-modifikatsii iz shtamma Citrobacter freundii 4111.