title: [Hydrolysis of 5'-phosphonates and nucleoside phosphates by phosphatases of various origin and human and calf serum].
abstract: The hydrolysis of 5'-phosphonates of 2'-deoxythymidine and its 3'-modified analogs, inhibiting the HIV reproduction, by the E. coli alkaline, calf intestine and human placenta phosphatases as well as by the Crotalus atrox venom 5'-nucleotidase were studied. Transformations of 5'-phosphonates of adenosine and its analogs during incubation with human and fetal calf blood sera were investigated. The nucleotide derivatives modified at the phosphate residue were not hydrolyzed by any of the phosphatases studied except for the cobra venom 5'-nucleotidase, the effectiveness of the latter depended on the substitutes at both phosphate and sugar residues. 2'-Deoxyadenosine incubation with blood sera resulted in its transformation to 2'-deoxyinosine and then to hypoxanthine. 2'-Deoxyadenosine 5'-phosphonates were stable during incubation with blood sera under the same conditions.
authors.forename: M K
authors.initials: MK
authors.lastname: Kukhanova
authors.forename: N B
authors.initials: NB
authors.lastname: Tarusova
authors.forename: M Ia
authors.initials: MIa
authors.lastname: Ias'ko
authors.forename: A A
authors.initials: AA
authors.lastname: Arzumanov
authors.forename: S O
authors.initials: SO
authors.lastname: Gudima
authors.forename: A A
authors.initials: AA
authors.lastname: Kraevskiĭ
authors.forename: Z G
authors.initials: ZG
authors.lastname: Chidzhavadze
authors.forename: R Sh
authors.initials: RSh
authors.lastname: Bibilashvili
mesh_terms: D015720:5'-Nucleotidase / Q000378:metabolism; D000818:Animals; D000998:Antiviral Agents / Q000378:metabolism*; D001769:Blood; D002417:Cattle; D003435:Crotalid Venoms / Q000201:enzymology; D004926:Escherichia coli / Q000201:enzymology; D006801:Humans; D006868:Hydrolysis; D007422:Intestines / Q000201:enzymology; D009682:Magnetic Resonance Spectroscopy; D009711:Nucleotides / Q000378:metabolism*; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism*; D010920:Placenta / Q000201:enzymology
chemical_list: D000998:Antiviral Agents; D003435:Crotalid Venoms; D009711:Nucleotides; D010744:Phosphoric Monoester Hydrolases; D015720:5'-Nucleotidase
vernacular_title: Gidroliz 5'-fosfonatov i fosfatov nukleozidov fosfatazami razlichnogo proiskhozhdeniia i syvorotkami krovi cheloveka i telenka.