title: Inhibition of vasopressin-induced formation of diradylglycerols in vascular smooth muscle cells by incorporation of eicosapentaenoic acid in membrane phospholipids.
abstract: OBJECTIVE
  Eicosapentaenoic acid and linoleic acid exert antihypertensive effects by an unknown mechanism unrelated to prostanoids, a property which is not shared by arachidonic acid. This study investigated the influence of these three acids on the formation of diradylglycerols and phosphatidic acid, key intracellular messengers involved in the mediation of agonist-induced vascular smooth muscle cell contraction.
  
  
  METHODS
  Rat mesenteric artery vascular smooth muscle cells in culture were pre-incubated for 24 h with eicosapentaenoic acid, linoleic acid or arachidonic acid. After thorough washing the cells were then incubated for 20 min in the presence of arginine vasopressin or vehicle, either immediately or following cell labelling with 32P-orthophosphate.
  
  
  METHODS
  The fatty acid composition of cell lipids was determined by gas chromatography after transesterification in the presence of boron trifluoride and methanol. Diradylglycerols and 32P-phosphatidic acid were purified from cell lipid extracts by thin-layer chromatography and diradylglycerols were analysed.
  
  
  RESULTS
  Incubation of vascular smooth muscle cells with eicosapentaenoic acid, linoleic acid or arachidonic acid resulted in the incorporation of these fatty acids at the sn-2 position of membrane phospholipids, mainly phosphatidylcholine and phosphatidylethanolamine. Eicosapentaenoic acid treatment was associated with a reduction, and linoleic acid treatment with an increase in the relative proportions of arachidonic acid found in cell phospholipids. Arginine vasopressin stimulated the formation of both diradylglycerols and 32P-phosphatidic acid. The arginine vasopressin-induced stimulation of diradylglycerols accumulation was almost completely abolished in eicosapentaenoic acid-treated cells, whereas it was not modified by linoleic acid or by arachidonic acid treatment. The arginine vasopressin-stimulated formation of 32P-phosphatidic acid was significantly inhibited by linoleic acid treatment but was not influenced by eicosapentaenoic acid or arachidonic acid treatment.
  
  
  CONCLUSIONS
  The incorporation of eicosapentaenoic acid or linoleic acid at the sn-2 position of membrane phospholipids leads to an inhibition of arginine vasopressin-induced formation of diradylglycerols or phosphatidic acid, respectively, in rat mesenteric artery vascular smooth muscle cells in culture. These properties may contribute to the antihypertensive effects in these fatty acids in vitro.
authors.affiliation: Institut de Recherches Cliniques, Montréal, Québec, Canada.
authors.forename: R
authors.initials: R
authors.lastname: Hui
authors.forename: M
authors.initials: M
authors.lastname: Robillard
authors.forename: P
authors.initials: P
authors.lastname: Falardeau
mesh_terms: D000818:Animals; D016718:Arachidonic Acid / Q000032:analysis / Q000378:metabolism; D001127:Arginine Vasopressin / Q000502:physiology*; D002478:Cells, Cultured; D004075:Diglycerides / Q000032:analysis / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D015118:Eicosapentaenoic Acid / Q000032:analysis / Q000378:metabolism*; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000032:analysis / Q000378:metabolism*; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000737:chemistry / Q000378:metabolism*; D010712:Phosphatidic Acids / Q000096:biosynthesis; D010743:Phospholipids / Q000737:chemistry / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D004075:Diglycerides; D008041:Linoleic Acids; D010712:Phosphatidic Acids; D010743:Phospholipids; D001127:Arginine Vasopressin; D016718:Arachidonic Acid; C035561:diarachidonyl diglyceride; D019787:Linoleic Acid; D015118:Eicosapentaenoic Acid