Inhibition of murine macrophage protein kinase C activity by nonviable Histoplasma capsulatum.
Ingestion of Histoplasma capsulatum yeast cells inhibits the oxidative burst response of murine macrophages (M phi's). Since protein kinase C (PKC) is believed to be an integral part of the signal transduction pathway involved in the production of reactive oxygen intermediates, we investigated the relationship between PKC activity and oxidative burst inhibition in H. capsulatum-containing murine peritoneal M phi's. An inhibitory effect on both basal and phorbol myristate acetate-mobilized membrane PKC activities was observed in M phi's which had ingested H. capsulatum but not latex spheres. These results suggest that one way in which H. capsulatum may disrupt the oxidative burst is through a PCK-dependent mechanism.
Wolf
J E
JE
Department of Medicine, Thomas Jefferson University, Philadelphia, Pennsylvania 19107.
Massof
S E
SE
Sherwin
J R
JR
Considine
R V
RV
D000284:Administration, Oral; D000595:Amino Acid Sequence; D000818:Animals; D005260:Female; D006658:Histoplasma / Q000378:metabolism*; D008264:Macrophages / Q000201:enzymology*; D051379:Mice; D008806:Mice, Inbred AKR; D008969:Molecular Sequence Data; D011493:Protein Kinase C / Q000378:metabolism*; D016897:Respiratory Burst / Q000276:immunology; D015398:Signal Transduction; D013481:Superoxides / Q000378:metabolism; D013997:Time Factors
D013481:Superoxides; D011493:Protein Kinase C