Effect of prostaglandin E2 (PGE2) and cyclic adenosine monophosphate (cAMP) upon actin cytoskeleton in human pulmonary fibroblasts (ICP-23) infected by measles virus.
The evolution of the actin cytoskeleton after trypsinization and recultivation as well as the effect of the PGE2 modulator and that of the secondary messenger, the cyclic AMP upon the same cytoskeletal proteins in human pulmonary fibroblasts (ICP-23) were studied. The substances were administered simultaneously and after one hour of viral adsorption. Using epifluorescence for pointing out filamentous actin the modifications occurring in this cytoskeletal protein when contacting trypsin and the virus and when PGE2 and cAMP are administered in the experimental variants are observed. Actin arrangement is obviously modified by the viral infection but the restrictive effect of PGE2 and cAMP upon virus replication is correlated with modifications occurring in the actin cytoskeleton.
Babeş
L
L
Cantacuzino Institute, Bucharest, Romania.
Gagheş
A
A
Orăşanu
M
M
Teletin
N
N
Petraşincu
D E
DE
Codău
M
M
D000199:Actins / Q000187:drug effects*; D002460:Cell Line; D002478:Cells, Cultured / Q000187:drug effects / Q000382:microbiology; D000242:Cyclic AMP / Q000494:pharmacology*; D003588:Cytopathogenic Effect, Viral / Q000187:drug effects; D003599:Cytoskeleton / Q000187:drug effects*; D015232:Dinoprostone / Q000494:pharmacology*; D005347:Fibroblasts / Q000187:drug effects / Q000382:microbiology; D006801:Humans; D008168:Lung / Q000166:cytology / Q000187:drug effects* / Q000382:microbiology; D008457:Measles / Q000382:microbiology*; D008459:Measles virus / Q000187:drug effects / Q000502:physiology; D013997:Time Factors; D014357:Trypsin / Q000494:pharmacology; D014779:Virus Replication / Q000187:drug effects
D000199:Actins; D000242:Cyclic AMP; D014357:Trypsin; D015232:Dinoprostone