The effects of hormonal and other stimuli on cell-surface Ro/SSA antigen expression by human keratinocytes in vitro: their possible role in the induction of cutaneous lupus lesions.
Ultraviolet B light (UVB) has previously been shown to induce the expression of the extractable nuclear antigens (e.g. Ro/SSA) on the surfaces of human keratinocytes in vitro. This study assessed whether injurious, metabolic, inflammatory, immunological or hormonal stimuli would also induce this expression or modulate that produced by UVB. No stimulus initiated expression alone, but 17-beta oestradiol doubled that found in response to UVB. These findings confirm the potential role of UVB in the initiation and potentiation of cutaneous lupus lesions and may help to explain the female preponderance of the disease.
Jones
S K
SK
Department of Dermatology, Bristol Royal Infirmary, U.K.
D000954:Antigens, Surface / Q000528:radiation effects*; D001324:Autoantigens / Q000528:radiation effects*; D002478:Cells, Cultured; D002772:Cholera Toxin / Q000494:pharmacology; D016207:Cytokines / Q000494:pharmacology; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D015603:Keratinocytes / Q000187:drug effects / Q000276:immunology / Q000528:radiation effects*; D007975:Leukotriene B4 / Q000494:pharmacology; D008180:Lupus Erythematosus, Systemic / Q000209:etiology*; D020733:RNA, Small Cytoplasmic; D012261:Ribonucleoproteins / Q000528:radiation effects*; D012737:Sex Factors; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014466:Ultraviolet Rays / Q000009:adverse effects*; D000099207:SS-A Antigen
D000954:Antigens, Surface; D001324:Autoantigens; D002772:Cholera Toxin; D016207:Cytokines; D004958:Estradiol; D006861:Hydrogen Peroxide; D007975:Leukotriene B4; D020733:RNA, Small Cytoplasmic; D012261:Ribonucleoproteins; D013755:Tetradecanoylphorbol Acetate; D000099207:SS-A Antigen; C116006:RO60 protein, human