Molecular cloning and characterization of the promoter region of the calcineurin A alpha gene.
The 5'-flanking region of the calcineurin A alpha gene was isolated from a rat genomic library. It lacked TATA and CAAT boxes but contained G+C-rich regions, and was demonstrated to function as a strong promoter in neuronal cell lines (NG108-15 mouse neuroblastoma x rat glioma hybrid cells or N1E115 mouse neuroblastoma cells), but not in nonneuronal cell lines (C6 rat glioma or L-M mouse fibroblastoid cells) in a transient chloramphenicol acetyltransferase expression assay. Deletion analysis of the 5'-flanking region revealed that the core promoter region, as well as the sequence critical for cell-type-specific-promoter function, reside within the fragment -107 to +157 with respect to the major transcription initiation site.
Chang
C D
CD
Department of Pharmacology, School of Medicine, Kobe University, Japan.
Takeda
T
T
Mukai
H
H
Shuntoh
H
H
Kuno
T
T
Tanaka
C
C
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D019703:Calcineurin; D002148:Calmodulin-Binding Proteins / Q000235:genetics*; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D017353:Gene Deletion; D016678:Genome; D005910:Glioma / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D009447:Neuroblastoma / Q000235:genetics; D009474:Neurons / Q000502:physiology; D010749:Phosphoprotein Phosphatases / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics*; D051381:Rats; D015183:Restriction Mapping; D014162:Transfection; D014407:Tumor Cells, Cultured
D002148:Calmodulin-Binding Proteins; D004247:DNA; D015500:Chloramphenicol O-Acetyltransferase; D019703:Calcineurin; D010749:Phosphoprotein Phosphatases