Cellular localization and characterization of proteins that bind high density lipoprotein.
High density lipoprotein (HDL) stimulates excretion of excess intracellular cholesterol from cells, presumably by interacting with a cell-surface receptor. A 110 kDa membrane protein that is a candidate for the HDL receptor has been identified by ligand blot analysis. In this study we determined the cellular localization of this and other HDL-binding proteins and characterized their properties. The plasma membranes (PM) of cultured bovine aortic endothelial cells were labeled with trace amounts of [3H]cholesterol, and cell homogenates were fractionated on sucrose and Percoll gradients. Ligand blot analysis of homogenates of cultured bovine aortic endothelial cells demonstrated that cells contain multiple proteins that bind HDL3, including a major membrane protein with an apparent M(r) of 110 kDa and two minor ones with M(r) of 105 and 130 kDa. The gradient distribution of the 105, 110, and 130 kDa HDL-binding proteins mirrored that of labeled cholesterol and 5'-nucleotidase, both PM markers. Treatment of intact cells with the water-soluble cross-linker bis(sulfosuccinimidyl)suberate abolished the HDL binding activity of the 110 and 130 kDa proteins but not that of the 105 kDa protein. These findings suggest that the 105, 110, and 130 kDa HDL-binding proteins are localized to the PM and that at least two of these proteins are exposed to the extracellular fluid. Solubilized 110 and 130 kDa proteins were retained on wheat-germ agglutinin and abrin lectin columns, showing that they are glycoproteins. The cellular localization and physical properties of the 110 and 130 kDa proteins suggest that they may play a role in binding of HDL to the cell surface.
Hokland
B
B
Department of Medicine, University of Washington, Seattle 98195.
Mendez
A J
AJ
Oram
J F
JF
D000036:Abrin; D000818:Animals; D001011:Aorta; D002352:Carrier Proteins; D002417:Cattle; D002458:Cell Fractionation; D002462:Cell Membrane / Q000737:chemistry; D002478:Cells, Cultured; D002846:Chromatography, Affinity; D003432:Cross-Linking Reagents / Q000494:pharmacology; D004730:Endothelium, Vascular / Q000737:chemistry; D008075:Lipoproteins, HDL / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D008970:Molecular Weight; D016601:RNA-Binding Proteins; D011956:Receptors, Cell Surface / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D018110:Receptors, Lipoprotein; D014909:Wheat Germ Agglutinins
D002352:Carrier Proteins; D003432:Cross-Linking Reagents; D008075:Lipoproteins, HDL; D008562:Membrane Glycoproteins; D016601:RNA-Binding Proteins; D011956:Receptors, Cell Surface; D018110:Receptors, Lipoprotein; D014909:Wheat Germ Agglutinins; C031616:high density lipoprotein receptors; D000036:Abrin; C075197:high density lipoprotein binding protein