title: Detection of human papillomavirus type 16 DNA sequences in archival cervical tissues by the polymerase chain reaction.
abstract: We have evaluated the polymerase chain reaction for the detection of viral DNA sequences in paraffin-embedded archival tissues. In 63 frozen cervical biopsy specimens that were taken from premalignant and invasive lesions, Southern blotting detected human papillomavirus (HPV) type 16 DNA in 28 (44%) of the samples. In the polymerase chain reaction analysis of the formalin-fixed, paraffin-embedded mirror biopsy specimens, 46 (73%) of the tissues were found to be positive for HPV type 16. In three Southern blotting-positive cases, the DNA of the paraffin-embedded sections was too scant or too degraded to allow the detection of HPV DNA by the polymerase chain reaction. In 21 Southern blotting-negative cases, HPV type 16 DNA could be demonstrated in the archival sections by the polymerase chain reaction technique--a sensitivity improvement of more than 80% over the standard method of HPV detection in tissues.
authors.affiliation: Department of Obstetrics and Gynecology, Rigshospitalet, University Hospital, Copenhagen, Denmark.
authors.forename: U
authors.initials: U
authors.lastname: Hørding
authors.forename: S
authors.initials: S
authors.lastname: Daugaard
authors.forename: A K
authors.initials: AK
authors.lastname: Iversen
authors.forename: J E
authors.initials: JE
authors.lastname: Bock
authors.forename: J
authors.initials: J
authors.lastname: Philip
mesh_terms: D001483:Base Sequence; D001706:Biopsy; D015139:Blotting, Southern; D002584:Cervix Uteri / Q000378:metabolism* / Q000473:pathology; D004279:DNA, Viral / Q000235:genetics / Q000378:metabolism*; D005260:Female; D006801:Humans; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes / Q000235:genetics; D027383:Papillomaviridae / Q000235:genetics*; D016133:Polymerase Chain Reaction
chemical_list: D004279:DNA, Viral; D015345:Oligonucleotide Probes