[Immunogenic proteins of nuclease-sensitive regions of chromatin of the normal rat liver and N-nitrosodiethylamine-induced hepatoma].
The localization of immunogenic proteins of chromatin in the nuclease-hypersensitive regions was investigated by means of polyclonal antibodies against the total chromatin from cells N-nitrosodiethylamine-induced hepatoma of rats and from hepatocytes. The fractions released after digestion by Ca-dependent endonuclease and by exogenous DNasa-1 were considerably enriched with immunogenic determinants. The enrichment of nuclease-hypersensitive sites of chromatin with tumour-associated antigens was observed in experiments with antihepatoma antibodies preincubated with chromatin from the normal liver cells. Higher immunoreactivity was typical of fractions obtained during Ca-dependent endonuclease segregation of the hepatoma chromatin, while products of digestion by exogenous DNasa-1 possessed higher tumour specificity.
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D000818:Animals; D002843:Chromatin / Q000737:chemistry*; D002868:Chromosomal Proteins, Non-Histone / Q000276:immunology*; D003850:Deoxyribonuclease I; D004052:Diethylnitrosamine; D004720:Endonucleases; D005092:Exonucleases; D008099:Liver / Q000737:chemistry*; D008114:Liver Neoplasms, Experimental / Q000139:chemically induced / Q000737:chemistry*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012016:Reference Values
D002843:Chromatin; D002868:Chromosomal Proteins, Non-Histone; D004052:Diethylnitrosamine; D004720:Endonucleases; D005092:Exonucleases; D003850:Deoxyribonuclease I
Immunogennye belki nukleaza-chuvstvitel'nykh oblasteĭ khromatina normal'noĭ pecheni krys i gepatomy, indutsirovannoĭ N-nitrozodiétilaminom.