Correction of human mucopolysaccharidosis type-VI fibroblasts with recombinant N-acetylgalactosamine-4-sulphatase.
A full-length human N-acetylgalactosamine-4-sulphatase (4-sulphatase) cDNA clone was constructed and expressed in CHO-DK1 cells under the transcriptional control of the Rous sarcoma virus long terminal repeat. A clonal cell line expressing high activities of human 4-sulphatase was isolated. The maturation and processing of the human enzyme in this transfected CHO cell line showed it to be identical with that seen in normal human skin fibroblasts. The high-uptake precursor form of the recombinant enzyme was purified from the medium of the transfected cells treated with NH4Cl and was shown to be efficiently endocytosed by control fibroblasts and by fibroblasts from a mucopolysaccharidosis type-VI (MPS VI) patient. Enzyme uptake was inhibitable by mannose 6-phosphate. After uptake, the enzyme was processed normally in both normal and MPS VI fibroblasts and was shown both to correct the enzymic defect and to initiate degradation of [35S]sulphated dermatan sulphate in MPS VI fibroblasts. The stabilities of the recombinant enzyme and enzyme from human fibroblasts appeared to be similar after uptake. However, endocytosed enzyme has a significantly shorter half-life than endogenous human enzyme. The purified precursor 4-sulphatase had a similar pH optimum and catalytic parameters to the mature form of 4-sulphatase isolated from human liver.
Anson
D S
DS
Department of Chemical Pathology, Adelaide Children's Hospital, South Australia.
Taylor
J A
JA
Bielicki
J
J
Harper
G S
GS
Peters
C
C
Gibson
G J
GJ
Hopwood
J J
JJ
D000469:Alkaline Phosphatase / Q000235:genetics; D000818:Animals; D001358:Avian Sarcoma Viruses / Q000235:genetics; D001483:Base Sequence; D016466:CHO Cells; D002478:Cells, Cultured; D002803:Chondro-4-Sulfatase / Q000172:deficiency / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D006224:Cricetinae; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D004705:Endocytosis; D005347:Fibroblasts / Q000201:enzymology; D006001:Glycoconjugates / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009087:Mucopolysaccharidosis VI / Q000378:metabolism*; D009838:Oligodeoxyribonucleotides; D011994:Recombinant Proteins / Q000378:metabolism; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D012867:Skin / Q000201:enzymology*; D014158:Transcription, Genetic; D014162:Transfection
D006001:Glycoconjugates; D009838:Oligodeoxyribonucleotides; D011994:Recombinant Proteins; D004247:DNA; D000469:Alkaline Phosphatase; D002803:Chondro-4-Sulfatase