title: Protonation states of the catalytic intermediates of cytochrome c oxidase.
abstract: Protonation changes accompanying conversion of oxidised (O state) cytochrome c oxidase to the 2-electron-reduced P state, and 3-electron-reduced F state at pH 8.0 have been measured. It was found that 2 and 3 protons, respectively, were taken up. The fourth proton required for the reduction of O2 to H2O must therefore be consumed in the remaining F----O portion of the catalytic cycle.
authors.affiliation: Glynn Research Institute, Bodmin, Cornwall, UK.
authors.forename: R
authors.initials: R
authors.lastname: Mitchell
authors.forename: P
authors.initials: P
authors.lastname: Mitchell
authors.forename: P R
authors.initials: PR
authors.lastname: Rich
mesh_terms: D000818:Animals; D002384:Catalysis; D002417:Cattle; D003576:Electron Transport Complex IV / Q000737:chemistry / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D011522:Protons
chemical_list: D011522:Protons; D003576:Electron Transport Complex IV