A highly conserved tyrosine residue at codon 845 within the kinase domain is not required for the transforming activity of human epidermal growth factor receptor.
Epidermal growth factor receptor (EGF-R) is a widely expressed ligand-dependent tyrosine kinase. The tyrosine residue at 845 in EGF-R corresponds to Y416 of v/c-src kinase, which is highly conserved and functionally important in many tyrosine kinases. To clarify the functional role of Y845, we constructed a mutant human EGF-R in which this tyrosine was replaced with phenylalanine and transfected it to NIH3T3 cells. EGF-R F845 induced EGF-dependent cellular transformation and revealed tyrosine-autophosphorylation of a 170 kDa protein, and initiated DNA synthesis similar to the wild-type EGF-R. We conclude here that Y845 is dispensable in the above mentioned functions of EGF-R tyrosine kinase.
Gotoh
N
N
Department of Genetics, University of Tokyo, Japan.
Tojo
A
A
Hino
M
M
Yazaki
Y
Y
Shibuya
M
M
D016475:3T3 Cells; D000818:Animals; D001483:Base Sequence; D003062:Codon / Q000235:genetics; D004261:DNA Replication; D004351:Drug Resistance / Q000235:genetics; D004815:Epidermal Growth Factor / Q000378:metabolism / Q000494:pharmacology*; D066246:ErbB Receptors / Q000235:genetics* / Q000378:metabolism; D005839:Gentamicins / Q000494:pharmacology; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009838:Oligodeoxyribonucleotides; D010649:Phenylalanine; D011505:Protein-Tyrosine Kinases / Q000235:genetics* / Q000378:metabolism; D014162:Transfection; D014443:Tyrosine
D003062:Codon; D005839:Gentamicins; D009838:Oligodeoxyribonucleotides; D014443:Tyrosine; D010649:Phenylalanine; D004815:Epidermal Growth Factor; C010680:antibiotic G 418; D066246:ErbB Receptors; D011505:Protein-Tyrosine Kinases