Chromosome mobilization of Legionella pneumophila with RK2::Mu and Tn5-Mob.
RK2::Mu plasmids and transposon Tn5-Mob were used to mobilize the Legionella pneumophila chromosome. Plate matings between L. pneumophila donors that contained RK2::Mu plasmids and auxotrophic recipients yielded recombinants at frequencies ranging from 10(-6) to 10(-7) per recipient for the markers tested. The presence of a Mu insertion in the chromosome of donors that harbored RK2::Mu plasmids increased the frequency of chromosome transfer of certain selected markers as compared with strains that contained RK2::Mu alone. Cotransfer experiments with Mu-containing donors and a thymidine and tryptophan auxotroph failed to reveal any linkage between the thy and trp loci in L. pneumophila. A strain that contained a chromosomal Tn5-Mob insertion and helper plasmid pRK24.4 transferred chromosomal markers at frequencies of 10(-7) per recipient. These findings suggest that RK2::Mu plasmids and Tn5-Mob may be useful for genetic mapping experiments with L. pneumophila.
Mintz
C S
CS
Department of Microbiology and Immunology, University of Miami, School of Medicine, FL 33101.
Zou
C H
CH
D015139:Blotting, Southern; D002876:Chromosomes, Bacterial / Q000378:metabolism*; D003227:Conjugation, Genetic / Q000235:genetics*; D004251:DNA Transposable Elements / Q000235:genetics*; D016952:Legionella pneumophila / Q000235:genetics*; D010957:Plasmids / Q000235:genetics*; D011995:Recombination, Genetic / Q000235:genetics
D004251:DNA Transposable Elements