DNA fragmentation in focal cortical freeze injury of rats.
This study examined the appearance of double-strand DNA breaks in rat brain after a focal cortical freeze injury in vivo. DNA fragments of oligonucleosome size appeared 3 h after the injury, and increased in a time-dependent manner. At 24 h, the amount of DNA fragmentation reached a maximum and then declined. When nuclei from freeze-injured brain tissue were incubated with Ca2+ in vitro, increased endonuclease activity, which can cause DNA fragmentation, was found. These findings indicate that the activation of a Ca(2+)-dependent endonuclease may be involved in the evolution of freeze-traumatized brain tissue.
Tominaga
T
T
Division of Neurosurgery, Tohoku University School of Medicine, Sendai, Japan.
Kure
S
S
Yoshimoto
T
T
D000818:Animals; D001930:Brain Injuries / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism; D002540:Cerebral Cortex / Q000378:metabolism* / Q000502:physiology; D004247:DNA / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D004720:Endonucleases / Q000378:metabolism; D005615:Freezing; D008297:Male; D009707:Nucleosomes / Q000737:chemistry; D051381:Rats; D011919:Rats, Inbred Strains
D009707:Nucleosomes; D004247:DNA; D004720:Endonucleases