Expression of gene-specific RNA in cultured cells exposed to rotating 60-Hz magnetic fields.
Replicate sets of cultures of mouse and of human cells were exposed to cyclic, sine wave, 60-Hz rotating magnetic fields of 1.0 Gs (1 Gs [symbol: see text] 0.1 mT) for 24-, 48-, or 72-h periods. Total RNA extracted from unexposed control and from magnetic field exposed cells was dot blot hybridized to a number of oncogene probes (including v-myc, v-fos, v-raf, and v-Ha-ras), a probe for 3611 murine sarcoma virus, a probe for the 70,000 dalton heat shock protein (hsp70), and a probe for the long terminal repeat sequence of mouse mammary tumor virus. Comparisons of levels of RNA in unexposed and magnetic field exposed cells measured by densitometer readings of resulting autoradiographs revealed no significant increases or decreases in RNA levels in magnetic field exposed cells with the seven probes tested.
Parker
J E
JE
Radiofrequency Radiation Branch, Armstrong Laboratory, Brooks Air Force Base, TX 78235-5301.
Winters
W
W
D000818:Animals; D015152:Blotting, Northern; D015342:DNA Probes / Q000235:genetics; D004574:Electromagnetic Fields; D015967:Gene Expression Regulation, Viral / Q000235:genetics*; D006360:Heat-Shock Proteins / Q000235:genetics*; D006801:Humans; D008324:Mammary Tumor Virus, Mouse / Q000235:genetics; D051379:Mice; D009857:Oncogenes / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics; D009053:Sarcoma Viruses, Murine / Q000235:genetics; D014407:Tumor Cells, Cultured
D015342:DNA Probes; D006360:Heat-Shock Proteins; D012367:RNA, Viral