Adhesion, growth, and matrix production by osteoblasts on collagen substrata.
A number of studies have demonstrated the pivotal role of collagen molecules in modulating cell growth and differentiation. In order to analyze the direct effects of collagen type I on the osteoblastic phenotype, we have devised an in vitro culture system for studying the interactions between bovine collagen type I and Saos-2 cells, a human osteoblastic cell line. Saos-2 cells were cultured both on top of collagen-coated culture dishes as well as inside a three-dimensional collagen network. Plating on dishes treated with collagen induced maximal adhesion of Saos-2 cells after 24-hour incubation. Cells cultured on collagen gel matrix expressed about 2.5-fold more alkaline phosphatase when compared with untreated plastic dishes. On collagen-coated dishes the responsiveness of Saos-2 cells to parathyroid hormone was decreased, whereas no modifications were observed in the effect of vasoactive intestinal peptide on these cells. Using a microfluorimetric measurement of DNA, an increase of proliferation was observed in Saos-2 cells cultured on collagen gel. Saos-2 cells were also able to colonize collagen sponges and in this three-dimensional network they were able to synthesize osteocalcin, as assessed both by immunocytochemistry and radioimmunoassay. In this study we have demonstrated that bovine collagen type I exhibits favorable effects on attachment and functional and growth activities of a human osteoblastic cell line, encouraging its use as a bone graft material.
Masi
L
L
Department of Clinical Physiopathology, University of Florence Viale Pieraccini 6 50139, Italy.
Franchi
A
A
Santucci
M
M
Danielli
D
D
Arganini
L
L
Giannone
V
V
Formigli
L
L
Benvenuti
S
S
Tanini
A
A
Beghè
F
F
D000469:Alkaline Phosphatase / Q000378:metabolism; D001859:Bone Neoplasms / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology; D002454:Cell Differentiation / Q000502:physiology; D002455:Cell Division / Q000187:drug effects / Q000502:physiology; D002471:Cell Transformation, Neoplastic / Q000473:pathology; D003094:Collagen; D000242:Cyclic AMP / Q000378:metabolism; D003592:Cytophotometry; D004273:DNA, Neoplasm / Q000032:analysis; D005109:Extracellular Matrix / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D005782:Gels; D006801:Humans; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D010006:Osteoblasts / Q000378:metabolism / Q000473:pathology* / Q000502:physiology; D015675:Osteocalcin / Q000032:analysis / Q000378:metabolism; D012516:Osteosarcoma / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology; D010281:Parathyroid Hormone / Q000494:pharmacology; D010641:Phenotype; D011863:Radioimmunoassay; D014407:Tumor Cells, Cultured / Q000737:chemistry / Q000473:pathology / Q000648:ultrastructure; D014660:Vasoactive Intestinal Peptide / Q000494:pharmacology
D004273:DNA, Neoplasm; D005782:Gels; D010281:Parathyroid Hormone; D015675:Osteocalcin; D014660:Vasoactive Intestinal Peptide; D003094:Collagen; D000242:Cyclic AMP; D000469:Alkaline Phosphatase