title: Radiation from ultraviolet phototherapy sources results in photodegradation of 12(R) and 12(S)-hydroxy-eicosatetraenoic acid: high-performance liquid chromatography and polymorph migration studies.
abstract: The effect of irradiation from Sylvania PUVA lamps (emitting predominantly in the ultraviolet (UVA) region) and broadband Philips TL-12 lamps (peaking in the UVB region) on two inflammatory mediators, 12(R)- and 12(S)-hydroxy-eicosatetraenoic acid (HETE) was studied. A high-performance liquid chromatography study showed significant photodegradation of both enantiomers at a concentration of 5 micrograms/ml in phosphate-buffered saline following irradiation with 10 J.cm-2 UVA or 0.375 J.cm-2 UVB. The in vitro chemokinetic microdroplet migration response of human peripheral polymorphonuclear leukocytes from normal and psoriatic subjects was significantly reduced following irradiation of 12(R)-HETE at a concentration of 1 micrograms/ml in medium with 40 J.cm-2 UVA and 1.5 J.cm-2 UVB respectively. No such effect was seen with 12(S)-HETE. The effect of ultraviolet on skin physiology, and in particular in the successful phototherapy of a range of inflammatory skin disorders, is not fully understood. The photodegradation of inflammatory mediators such as 12-HETE, as shown in this study, provides another factor of possible therapeutic significance.
authors.affiliation: Department of Dermatology, Ninewells Hospital and Medical School, Dundee, United Kingdom.
authors.forename: I
authors.initials: I
authors.lastname: Amorim
authors.forename: C
authors.initials: C
authors.lastname: Green
authors.forename: A
authors.initials: A
authors.lastname: Hill
authors.forename: T M
authors.initials: TM
authors.lastname: MacLeod
authors.forename: J
authors.initials: J
authors.lastname: Ferguson
mesh_terms: D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D006893:Hydroxyeicosatetraenoic Acids / Q000008:administration & dosage / Q000032:analysis / Q000494:pharmacology / Q000528:radiation effects*; D009504:Neutrophils / Q000187:drug effects; D011701:PUVA Therapy / Q000295:instrumentation*; D011565:Psoriasis / Q000097:blood; D013057:Spectrum Analysis; D014466:Ultraviolet Rays; D014467:Ultraviolet Therapy / Q000295:instrumentation*
chemical_list: D006893:Hydroxyeicosatetraenoic Acids; D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid