Glucose-6-phosphatase in normal adult human intestinal mucosa.
1. The existence of specific glucose-6-phosphatase activity in human intestinal mucosa has been somewhat controversial. 2. We have demonstrated the presence of low levels of specific glucose-6-phosphatase activity in normal human adult intestinal mucosa. Activity was found in oesophagus, stomach, duodenum and colon. 3. Immunoblot analysis using antibodies monospecific for the 36.5 kDa liver glucose-6-phosphatase catalytic subunit demonstrated that intestinal mucosa contains low levels of the glucose-6-phosphatase enzyme protein. 4. The low levels of activity together with problems of proteolysis make human intestinal biopsies unsuitable for use in the diagnosis of type 1 glycogen-storage disease.
Pears
J
J
Department of Medicine, Ninewells Hospital and Medical School, Dundee, U.K.
Jung
R T
RT
Jankowski
J
J
Waddell
I D
ID
Burchell
A
A
D000328:Adult; D004947:Esophagus / Q000201:enzymology; D005753:Gastric Mucosa / Q000201:enzymology; D005952:Glucose-6-Phosphatase / Q000378:metabolism*; D006801:Humans; D007413:Intestinal Mucosa / Q000201:enzymology*; D008861:Microsomes / Q000201:enzymology; D013379:Substrate Specificity
D005952:Glucose-6-Phosphatase