title: Evidence for independent molecular identity and functional interaction of the haemagglutinin and cysteine proteinase (gingivain) of Porphyromonas gingivalis.
abstract: The sequence of events involved in haemagglutination and lysis of erythrocytes by washed cells, vesicles and the culture supernate of Porphyromonas gingivalis strain W83 was monitored by 51Cr release and transmission electronmicroscopy. All preparations, except capsular material and lipopolysaccharide, caused haemagglutination and, by a slow process of attachment and specific attack on the surface structures of the red blood cells, produced minute pores and eventual leakage of cellular contents. N-acetylglucosamine, N-acetylgalactosamine and several other sugars such as glucose and sucrose had no effect on haemagglutination. Antiserum raised against a cloned haemagglutinin of P. gingivalis strain 381 inhibited the activity of strain W83 cells, vesicles and supernate. The antiserum-neutralised supernate lost 70-80% of its hydrolytic activity towards alpha-N-benzoyl-L-arginine-4-nitroanilide but the residual activity behaved in a manner similar to the native supernate in that it was completely inhibited by the addition of 2,2'-dipyridyl disulphide and was fully restored upon addition of a low-Mr mercaptan. Binding of the antiserum to the haemagglutinin epitope of P. gingivalis still permitted titration of the active centre cysteinyl thiol group of the proteinase. Purified gingivain caused lysis of erythrocytes and was not neutralised by antiserum to the haemagglutinin. These results suggest that, although the haemagglutinin and gingivain are probably separate molecules, they are closely associated on the outer membrane of P. gingivalis and may be functionally related.
authors.affiliation: Department of Oral Microbiology, London Hospital Medical College, University of London, Whitechapel.
authors.forename: H N
authors.initials: HN
authors.lastname: Shah
authors.forename: S E
authors.initials: SE
authors.lastname: Gharbia
authors.forename: A
authors.initials: A
authors.lastname: Progulske-Fox
authors.forename: K
authors.initials: K
authors.lastname: Brocklehurst
mesh_terms: D015082:2,2'-Dipyridyl / Q000031:analogs & derivatives / Q000494:pharmacology; D000818:Animals; D001586:Benzoylarginine Nitroanilide / Q000378:metabolism; D002241:Carbohydrates / Q000494:pharmacology; D003546:Cysteine Endopeptidases / Q000378:metabolism*; D004220:Disulfides / Q000494:pharmacology; D004912:Erythrocytes / Q000648:ultrastructure; D006384:Hemagglutination; D006388:Hemagglutinins / Q000276:immunology / Q000378:metabolism*; D006461:Hemolysis; D006863:Hydrogen-Ion Concentration; D007106:Immune Sera; D008854:Microscopy, Electron; D016966:Porphyromonas gingivalis / Q000737:chemistry* / Q000201:enzymology / Q000472:pathogenicity; D012756:Sheep
chemical_list: D002241:Carbohydrates; D004220:Disulfides; D006388:Hemagglutinins; D007106:Immune Sera; C001105:2,2'-dipyridyl disulfide; D015082:2,2'-Dipyridyl; D001586:Benzoylarginine Nitroanilide; D003546:Cysteine Endopeptidases; C067046:gingivain