title: In vitro inhibition of 5-lipoxygenase metabolite, leukotriene B4, in bovine mononuclear cells by bovine viral diarrhea virus.
abstract: Bovine viral diarrhea (BVD) virus inhibited phytohemagglutinin (PHA)-, PHA plus phorbol-12-myristate-13 acetate (PMA)- or PHA plus calcium ionophore (A23187)-stimulated bovine peripheral blood mononuclear cell (PBMC) proliferation. Further, BVD-virus inhibited A23187-stimulated leukotriene B4 (LTB4) synthesis into the culture supernatants. Presence of exogenous LTB4 failed to reverse the BVD virus-induced immunosuppression. Our results suggest that BVD virus-induced immunosuppression is due to a factor that may be necessary to induce LTB4 synthesis for normal mononuclear cell proliferation.
authors.affiliation: Department of Anatomy and Physiology, Kansas State University, Manhattan 66506.
authors.forename: D
authors.initials: D
authors.lastname: Atluru
authors.forename: S
authors.initials: S
authors.lastname: Gudapaty
authors.forename: W
authors.initials: W
authors.lastname: Xue
authors.forename: F
authors.initials: F
authors.lastname: Gurria
authors.forename: M M
authors.initials: MM
authors.lastname: Chengappa
authors.forename: D S
authors.initials: DS
authors.lastname: McVey
authors.forename: H C
authors.initials: HC
authors.lastname: Minocha
authors.forename: S
authors.initials: S
authors.lastname: Atluru
mesh_terms: D000818:Animals; D001094:Arachidonate 5-Lipoxygenase / Q000378:metabolism*; D000001:Calcimycin / Q000494:pharmacology; D002417:Cattle; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D001908:Diarrhea Viruses, Bovine Viral / Q000502:physiology*; D007165:Immunosuppression Therapy; D007963:Leukocytes, Mononuclear / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology; D007975:Leukotriene B4 / Q000037:antagonists & inhibitors*; D008213:Lymphocyte Activation; D008934:Mitogens / Q000494:pharmacology; D011863:Radioimmunoassay
chemical_list: D008934:Mitogens; D007975:Leukotriene B4; D000001:Calcimycin; D001094:Arachidonate 5-Lipoxygenase