Purification and crystallization of insecticidal delta-endotoxin CryIIIB2 from Bacillus thuringiensis.
CryIIIB2, an insecticidal protein from Bacillus thuringiensis has been crystallized from 0.6 M NaBr and HEPES buffer at pH 7.0 and X-ray diffraction data collected on a native crystal to 2.4 A. The insecticidal protein was obtained from a Bacillus thuringiensis (Bt) strain EG7231. Crystals of the endotoxin are orthorhombic, space group C2221, with unit cell dimensions of a = 122.44, b = 131.81, and c = 105.37 A. A unit cell contains one molecule of the 67,000 Da endotoxin per asymmetric unit.
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Molecular Biophysics Department, Medical Foundation of Buffalo, New York 14203.
Luft
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Jensen
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Pangborn
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English
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D001413:Bacillus thuringiensis / Q000737:chemistry*; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D003460:Crystallization; D004731:Endotoxins / Q000737:chemistry / Q000302:isolation & purification*; D006460:Hemolysin Proteins; D007306:Insecticides / Q000737:chemistry / Q000302:isolation & purification*
D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004731:Endotoxins; D006460:Hemolysin Proteins; D007306:Insecticides; C030956:insecticidal crystal protein, Bacillus Thuringiensis