Expression of interleukin 5 by the CD4+CD45R0+ subset of human T cells.
The expression of IL5 by CD4+CD45RA+, CD4+CD45R0+ and CD3+CD8+ subsets of human peripheral blood mononuclear cells was assessed. Interleukin 5 expression was detected by RNA extraction, reverse transcription and polymerase chain reaction. Populations of highly purified cells were obtained by a protocol of sequential plastic adherence, magnetic bead separation and flow cytometric cell sorting. IL5 was clearly expressed in the CD4+CD45R0+ subset from 3 to 48 hr after activation. The CD4+CD45RA+ and CD3+CD8+ subsets expressed very much less IL5. By contrast, IL2 expression was readily detected in all sorted populations. Thus, in activated CD4+ cells, IL5 was predominantly expressed in the CD4+CD45R0+ subset, a pattern of expression corresponding to that reported for a number of other cytokines, and differing from that of IL2.
Sewell
W A
WA
Centre for Immunology, St. Vincent's Hospital, Sydney, Australia.
Valentine
J E
JE
Cooley
M A
MA
D015703:Antigens, CD / Q000032:analysis*; D001483:Base Sequence; D015704:CD4 Antigens / Q000032:analysis; D002469:Cell Separation; D017931:DNA Primers; D005434:Flow Cytometry; D015870:Gene Expression; D006801:Humans; D007376:Interleukin-2 / Q000096:biosynthesis; D015848:Interleukin-5 / Q000096:biosynthesis*; D017493:Leukocyte Common Antigens / Q000032:analysis; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D016176:T-Lymphocyte Subsets / Q000276:immunology / Q000378:metabolism*
D015703:Antigens, CD; D015704:CD4 Antigens; D017931:DNA Primers; D007376:Interleukin-2; D015848:Interleukin-5; D012333:RNA, Messenger; D017493:Leukocyte Common Antigens