Human Y-79 retinoblastoma cells express functional corticotropin-releasing hormone receptors.
In human Y-79 retinoblastoma cells corticotropin-releasing hormone (CRH) produces a marked and rapid increase of adenylate cyclase activity. The concentration of the peptide producing half-maximal stimulation is 60 nM. The effect of CRH is significantly antagonized by the specific CRH receptor antagonist alpha-helical CHR 9-41 and is mimicked by sauvagine and urotensin I, two peptides displaying sequence homology with CRH. These results demonstrate the presence of functional CRH receptors in human Y-79 retinoblastoma cells and suggest that this cell line may be a suitable model in which to study the action of CRH on human retinal cell function.
Olianas
M C
MC
Department of Neurosciences, University of Cagliari, Italy.
Lampis
G
G
Onali
P
P
D000262:Adenylyl Cyclases / Q000378:metabolism*; D002460:Cell Line; D003346:Corticotropin-Releasing Hormone / Q000037:antagonists & inhibitors / Q000378:metabolism / Q000494:pharmacology*; D005134:Eye Neoplasms; D006801:Humans; D007700:Kinetics; D010446:Peptide Fragments / Q000494:pharmacology; D018019:Receptors, Corticotropin-Releasing Hormone; D017981:Receptors, Neurotransmitter / Q000378:metabolism / Q000502:physiology*; D012175:Retinoblastoma
D010446:Peptide Fragments; D018019:Receptors, Corticotropin-Releasing Hormone; D017981:Receptors, Neurotransmitter; D003346:Corticotropin-Releasing Hormone; C045089:corticotropin releasing hormone (9-41); D000262:Adenylyl Cyclases