Membrane vesicles from brown adipose tissue: a tool for the study of amino acid transport. The case of L-alanine.
A density gradient method is used to isolate membrane vesicles from brown adipose tissue. These respond to changes in osmolarity and show the classical overshoot pattern when L-alanine uptake is assayed. Transport is shown to be effected by two components: a linear (Kd = 0.498 min-1) and Na(+)-dependent saturable component (Km = 2.3 mM) and a Vmax = 19.9 pmol/micrograms protein.min). This pattern is similar to that shown by cells isolated from brown adipose tissue.
Rodríguez-Martín
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Departament de Bioquímica i Fisiologia, Universitat de Barcelona, Spain.
Bel
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Remesar
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D015720:5'-Nucleotidase / Q000032:analysis; D002001:Adipose Tissue, Brown / Q000378:metabolism* / Q000648:ultrastructure; D000409:Alanine / Q000378:metabolism*; D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D001692:Biological Transport; D002458:Cell Fractionation / Q000379:methods; D003576:Electron Transport Complex IV / Q000032:analysis; D005260:Female; D007700:Kinetics; D008861:Microsomes / Q000378:metabolism; D009247:NADH, NADPH Oxidoreductases / Q000032:analysis; D009995:Osmosis; D051381:Rats; D017208:Rats, Wistar; D015091:beta-Alanine / Q000031:analogs & derivatives / Q000494:pharmacology
D000596:Amino Acids; D015091:beta-Alanine; C012924:2,2-dimethyl-beta-alanine; D009247:NADH, NADPH Oxidoreductases; D003576:Electron Transport Complex IV; D015720:5'-Nucleotidase; D000409:Alanine