title: Differential effect of basolateral and apical adenosine on AVP-stimulated cAMP formation in primary culture of IMCD.
abstract: It has been recently established that adenosine interferes with the ability of arginine vasopressin (AVP) to generate adenosine 3',5'-cyclic monophosphate (cAMP) in inner medullary collecting duct (IMCD) cells in culture. The aim of the current study was to determine whether this interaction of adenosine with AVP is mediated by adenosine from the basolateral (B) and/or the apical (A) surface of the tubule cell. Cells from rat IMCD were grown to confluence in monolayers on porous filters. Adenosine (5 x 10(-8)-10(-4) M) applied to the B or A surface of the cell had no detectable effect on basal cAMP formation. AVP, 10(-9)-10(-6) M, increased cAMP formation from both B and A surfaces of the cell. When AVP was applied to the B surface, 10(-6) M adenosine inhibited AVP-stimulated cAMP formation from the B side only, whereas adenosine at 10(-4) M inhibited cAMP formation from both B and A sides. The inhibitory effect of adenosine was reproduced with N6-cyclohexyladenosine (CHA) from both B and A surfaces. 5'-(N-ethylcarboxamido)adenosine (NECA) and 2',5'-dideoxyadenosine (DDA) inhibited cAMP formation from the B surface only. When AVP was applied to the A surface, the inhibitory effects of adenosine were the same as when AVP was applied to the B surface; CHA, NECA, and DDA inhibited AVP-stimulated cAMP formation from both the B and A surfaces. 1,3-Dipropyl-8-cyclopentylxanthine (DPCPX), an adenosine antagonist with selectivity for the A1 receptor, prevented the inhibitory effects of adenosine, CHA, and NECA on AVP-stimulated cAMP formation.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: University of Rochester School of Medicine and Dentistry, New York 14642.
authors.forename: Y
authors.initials: Y
authors.lastname: Yagil
mesh_terms: D000241:Adenosine / Q000031:analogs & derivatives / Q000378:metabolism* / Q000493:pharmacokinetics; D000818:Animals; D001127:Arginine Vasopressin / Q000494:pharmacology*; D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000096:biosynthesis*; D004305:Dose-Response Relationship, Drug; D004594:Electrophysiology; D007679:Kidney Medulla; D007685:Kidney Tubules, Collecting / Q000166:cytology / Q000378:metabolism* / Q000502:physiology; D009994:Osmolar Concentration; D010539:Permeability; D051381:Rats; D011919:Rats, Inbred Strains; D014018:Tissue Distribution; D014970:Xanthines / Q000494:pharmacology
chemical_list: D014970:Xanthines; D001127:Arginine Vasopressin; C051360:1,3-dipropyl-8-cyclopentylxanthine; D000242:Cyclic AMP; D000241:Adenosine