title: Formation of a covalent Hg-Cys-bond during mercurial activation of PMNL procollagenase gives evidence of a cysteine-switch mechanism.
abstract: A common method for the activation of mammalian metalloproteinases is the use of mercurial compounds. Activation of PMNL procollagenase by soluble mercurials takes place as a three-step mechanism with a final intermolecular loss of the PRCGVPD autoinhibitor region. In this study covalently bound mercury in the form of mercurial agarose was chosen to probe activation of PMNL procollagenase. Activation was not achieved, since the final intermolecular cleavage with removal of the PRCGVPD motif could not take place. An intermediate form of the enzyme was bound to the column. Its N-terminal sequence determination proved cleavage of the Asp64-Met65 peptide bond leaving the cysteine of the propeptide domain for covalent attachment to the mercurial agarose. This gives further evidence of a cysteine-switch mechanism involving Cys71.
authors.affiliation: Department of Biochemistry, University of Bielefeld, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Bläser
authors.forename: S
authors.initials: S
authors.lastname: Triebel
authors.forename: H
authors.initials: H
authors.lastname: Reinke
authors.forename: H
authors.initials: H
authors.lastname: Tschesche
mesh_terms: D000595:Amino Acid Sequence; D017364:Collagenases; D003545:Cysteine / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D004789:Enzyme Activation; D004792:Enzyme Precursors / Q000378:metabolism*; D006801:Humans; D008627:Mercuric Chloride; D008628:Mercury / Q000378:metabolism*; D003012:Microbial Collagenase / Q000378:metabolism*; D008969:Molecular Sequence Data; D009504:Neutrophils / Q000201:enzymology*
chemical_list: D004792:Enzyme Precursors; D008627:Mercuric Chloride; D017364:Collagenases; C022440:procollagenase; D003012:Microbial Collagenase; D008628:Mercury; D003545:Cysteine