Glucocorticoid, androgen, and retinoic acid regulation of glutathione S-transferase gene expression in hamster smooth muscle tumor cells.
A mu class glutathione S-transferase gene (hGSTYBX) is expressed in the DDT1MF-2 hamster smooth muscle tumor cell line. This gene is glucocorticoid responsive, and near maximal induction was found to occur within 24 h. The induced mRNA was very stable with a half-life of more than 48 h. Serum had no effect on either constitutive or glucocorticoid induced hGSTYBX expression. Although dibutyryl cAMP, phenobarbital, and 12-O-tetradecanoylphorbol-13-acetate did not alter hGSTYBX expression, testosterone and retinoic acid were each able to increase hGSTYBX expression in a concentration dependent manner. These results demonstrate a unique pattern of responsiveness of the hamster gene compared to the glutathione S-transferase genes of other species.
Schwartz
D A
DA
Department of Medicine, Medical University of South Carolina, Charleston 29425.
Norris
J S
JS
D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D002460:Cell Line; D006224:Cricetinae; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D005982:Glutathione Transferase / Q000235:genetics*; D007527:Isoenzymes / Q000235:genetics*; D009130:Muscle, Smooth; D009374:Neoplasms, Experimental / Q000201:enzymology* / Q000235:genetics; D010634:Phenobarbital / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013739:Testosterone / Q000494:pharmacology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014212:Tretinoin / Q000494:pharmacology*; D014222:Triamcinolone Acetonide / Q000494:pharmacology*
D007527:Isoenzymes; D012333:RNA, Messenger; D013739:Testosterone; D014212:Tretinoin; D003994:Bucladesine; D005982:Glutathione Transferase; D014222:Triamcinolone Acetonide; D013755:Tetradecanoylphorbol Acetate; D010634:Phenobarbital