title: Evidence for de novo rearrangements of Drosophila transposable elements induced by the passage to the cell culture.
abstract: The genomic distribution and the number of elements of eleven transposon families have been compared by the Southern technique between permanent cultured cells, larval salivary glands and the brains and whole flies of an inbred Drosophila line (inb-c) from which the cells were established. In cultured cells, changes in restriction patterns consistent with various types of rearrangements such as amplification, transposition and excision of the elements of copia, 1731, 412, 297 and mdg-4 transposon families are detected whereas B 104, G and blood elements appear stable. In previous reports these rearrangements were not detected among individuals of the inb-c line or among samples of somatic tissues, or in samples spanning years of maintenance of cultured cells. Hence, we believe that they have been induced de novo during the passage to the cell culture.
authors.affiliation: Centro per lo Studio degli Acidi Nucleici C.N.R., Roma, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Di Franco
authors.forename: C
authors.initials: C
authors.lastname: Pisano
authors.forename: F
authors.initials: F
authors.lastname: Fourcade-Peronnet
authors.forename: G
authors.initials: G
authors.lastname: Echalier
authors.forename: N
authors.initials: N
authors.lastname: Junakovic
mesh_terms: D000818:Animals; D001345:Autoradiography; D002478:Cells, Cultured; D015342:DNA Probes; D004251:DNA Transposable Elements; D004330:Drosophila / Q000235:genetics*; D015321:Gene Rearrangement
chemical_list: D015342:DNA Probes; D004251:DNA Transposable Elements