title: Expression of the protease gene of equine infectious anemia virus in Escherichia coli: formation of the mature processed enzyme and specific cleavage of the gag precursor.
abstract: A 620-bp Bg/II restriction fragment containing the putative protease coding sequence from equine infectious anemia virus (EIAV) proviral DNA was cloned and expressed in E. coli as a Pol precursor protein. In contrast to the 25-kDa fusion protein predicted from the expressed pol sequence, a protein of approximately 10 kDa was generated by apparent autocatalytic processing of the Pol precursor. This mature processed protein was detected in transformed cells using an antisera raised against synthetic peptide from the conserved carboxyl-terminal segment of the predicted EIAV protease coding sequence. Coexpression of this protein with a 35-kDa EIAV Gag-precursor fusion protein resulted in the specific proteolytic processing of the precursor as shown by formation of p26, the major capsid protein of EIAV.
authors.affiliation: Department of Biochemistry, Louisiana State University, Baton Rouge.
authors.forename: K
authors.initials: K
authors.lastname: Rushlow
authors.forename: X X
authors.initials: XX
authors.lastname: Peng
authors.forename: R C
authors.initials: RC
authors.lastname: Montelaro
authors.forename: D S
authors.initials: DS
authors.lastname: Shih
mesh_terms: D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D005786:Gene Expression Regulation; D015683:Gene Products, gag / Q000235:genetics / Q000378:metabolism; D015684:Gene Products, pol / Q000235:genetics* / Q000378:metabolism; D004860:Infectious Anemia Virus, Equine / Q000201:enzymology / Q000235:genetics*; D011498:Protein Precursors / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D015531:Radioimmunoprecipitation Assay; D015183:Restriction Mapping
chemical_list: D015683:Gene Products, gag; D015684:Gene Products, pol; D011498:Protein Precursors