Measurement of MPO activity as model for detection of granulocyte infiltration in different tissues.
Activity of myeloperoxidase (MPO) was determined in different tissues to detect granulocyte infiltration. MPO was measured in the mouse ear after injection of interleukin-1 beta, in the rat paw after carrageenan-induced edema and in the lung of sensitized guinea pigs after ovalbumin inhalation. Pretreatment of the animals with antiinflammatory drugs abolished the increase of MPO activity in tissues induced by this different stimuli.
Werner
U
U
Department of Pharmacology, ASTA Medica AG, Frankfurt/Main, FRG.
Szelenyi
I
I
D000818:Animals; D002351:Carrageenan / Q000633:toxicity; D003907:Dexamethasone / Q000494:pharmacology; D004423:Ear; D004487:Edema / Q000139:chemically induced / Q000188:drug therapy / Q000276:immunology; D006168:Guinea Pigs; D006614:Hindlimb; D007213:Indomethacin / Q000494:pharmacology; D007249:Inflammation / Q000276:immunology*; D007375:Interleukin-1 / Q000494:pharmacology; D008168:Lung / Q000276:immunology; D008297:Male; D051379:Mice; D009504:Neutrophils / Q000201:enzymology / Q000276:immunology*; D010047:Ovalbumin / Q000008:administration & dosage; D009195:Peroxidase / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
D007375:Interleukin-1; D003907:Dexamethasone; D002351:Carrageenan; D010047:Ovalbumin; D009195:Peroxidase; D007213:Indomethacin