Increasing the efficiency of lipid-conjugated antibodies incorporation into the membrane of antigen presenting B cells.
We have introduced some modifications in the technique called "cell decoration" in order to increase the amount of lipid-conjugated antibodies which can be incorporated into the membrane of B cells. As shown by FACS analysis, we have obtained an approximately 4-fold increment in the amount of specific antibodies incorporated into the cell membrane. The procedure, which consists of successive changes of the medium that contains the lipid-conjugated antibodies, avoided changes on parameters that interfere with cell viability. The proposed modification resulted in an approximately 2-fold enhancement of the ability of decorated B cells to act as antigen presenting cells for specific T hybridomas.
Gontijo
C M
CM
Departamento de Bioquímica e Imunologia, Universidade Federal de Minas Gerais, Belo Horizonte, Brasil.
Möller
G
G
D000818:Animals; D000906:Antibodies / Q000187:drug effects / Q000276:immunology*; D000918:Antibody Specificity / Q000187:drug effects / Q000276:immunology; D000938:Antigen-Presenting Cells / Q000187:drug effects / Q000276:immunology*; D001402:B-Lymphocytes / Q000187:drug effects / Q000276:immunology*; D002462:Cell Membrane / Q000187:drug effects / Q000276:immunology; D003840:Deoxycholic Acid; D007158:Immunologic Techniques; D007202:Indicators and Reagents; D008563:Membrane Lipids / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D011946:Receptors, Antigen / Q000187:drug effects / Q000276:immunology; D012995:Solubility
D000906:Antibodies; D007202:Indicators and Reagents; D008563:Membrane Lipids; D011946:Receptors, Antigen; D003840:Deoxycholic Acid