title: Structure and expression of a gene from Arabidopsis thaliana encoding a protein related to SNF1 protein kinase.
abstract: The AKin10 gene from Arabidopsis thaliana encoding a putative Ser/Thr protein kinase (PK) has been isolated and characterized. The AKin10-encoding gene is located on a genomic 5.4-kb BamHI fragment and contains ten introns, one being located in the 5' untranslated region. The deduced amino acid sequence of AKin10 is 65% identical over the catalytic domain to the yeast PK (SNF1). SNF1 is essential for the derepression of many glucose-repressible genes, including Suc2 which encodes invertase. Southern blot hybridization experiments suggested the presence of one copy of the gene per haploid genome of A. thaliana. Northern hybridization experiments indicated that this gene is expressed in roots, shoots and leaves. AKin10 may play an important role in a signal transduction cascade regulating gene expression and carbohydrate metabolism in higher plants.
authors.affiliation: Institut de Recherches sur les Plantes URA 1128, Université de Paris-XI, Orsay, France.
authors.forename: L
authors.initials: L
authors.lastname: Le Guen
authors.forename: M
authors.initials: M
authors.lastname: Thomas
authors.forename: M
authors.initials: M
authors.lastname: Bianchi
authors.forename: N G
authors.initials: NG
authors.lastname: Halford
authors.forename: M
authors.initials: M
authors.lastname: Kreis
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000235:genetics*; D001483:Base Sequence; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D005091:Exons; D005656:Fungal Proteins / Q000235:genetics; D015723:Gene Library; D017343:Genes, Plant; D007438:Introns; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010940:Plant Proteins / Q000235:genetics*; D011494:Protein Kinases / Q000235:genetics*; D017346:Protein Serine-Threonine Kinases / Q000235:genetics*; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid
chemical_list: D029681:Arabidopsis Proteins; D004247:DNA; D005656:Fungal Proteins; D010940:Plant Proteins; D011494:Protein Kinases; D017346:Protein Serine-Threonine Kinases; C071570:SNF1-related protein kinases; C531469:At3g01090 protein, Arabidopsis