title: Comparison of MRC-5 and continuous cell lines for detection of cytomegalovirus in centrifugation cultures.
abstract: Continuous cell lines were assessed for use for rapid human cytomegalovirus (HCMV) detection procedures combining tissue culture, centrifugation, and immediate early antigen (IEA) immunostaining. Human cells (MRC-5 embryonic fibroblasts, U-373MG astrocytoma cells, differentiated teratocarcinoma (Tera-2) cells), murine cells (BALB/c-3T3 and Y-1 cells), BHK21 hamster cells, and mink lung (ML) cells were first inoculated with HCMV laboratory strain. IEA synthesizing cells were detected by immunoperoxidase assay using a monoclonal antibody. ML cells and differentiated Tera-2 cells exhibited more positive cells than MRC-5 cells. BHK21, and MRC-5 cells were equivalent in sensitivity whereas U-373MG, BALB/c-3T3, and Y-1 cells had only reduced IEA positive cells. When 63 urine specimens were inoculated onto MRC-5, ML and differentiated Tera-2 cells, 20 (31.7%) were positive in MRC-5 cells versus 18 (28.5%) in ML or Tera-2 cells. Moreover, greater numbers of infected cells were detected in MRC-5 cells than in these two cell lines. MRC-5 cells were superior for detection of HCMV in clinical samples by centrifugation cultures.
authors.affiliation: Service de Bactériologie-Virologie, Hôpital Saint-Louis, Université Paris VII, France.
authors.forename: M C
authors.initials: MC
authors.lastname: Mazeron
authors.forename: B
authors.initials: B
authors.lastname: Benjelloun
authors.forename: C
authors.initials: C
authors.lastname: Bertrand
authors.forename: J L
authors.initials: JL
authors.lastname: Pons
authors.forename: Y
authors.initials: Y
authors.lastname: Pérol
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D002455:Cell Division; D002460:Cell Line; D006224:Cricetinae; D003587:Cytomegalovirus / Q000254:growth & development* / Q000302:isolation & purification*; D006801:Humans; D017874:Immediate-Early Proteins; D051379:Mice; D013045:Species Specificity; D014407:Tumor Cells, Cultured; D014556:Urine / Q000382:microbiology
chemical_list: D000956:Antigens, Viral; D017874:Immediate-Early Proteins; C044582:immediate-early proteins, cytomegalovirus