Detection of free radicals in UV-irradiated lens by spin trapping ESR.
We have made an ESR study on the UV-photolysis of lens and identified the origins of free radicals involved in the initial photochemical process by spin trapping technique. Two spin adducts were detected on irradiation of canine lens in the presence of a spin trapping reagent (DMPO); a spin adduct of sulfur centered radical derived from glutathione and the protonated adduct of hydrated electron. A free radical mechanism of initial photochemical injury in UV-irradiated lens was discussed, comparing with a photolysis of tryptophan plus cysteine solution.
Murakami
J
J
Kozuka Eye Clinic, Ehime, Japan.
Kozuka
Y
Y
Okazaki
M
M
Shiga
T
T
D000818:Animals; D003497:Cyclic N-Oxides; D003545:Cysteine / Q000528:radiation effects; D004285:Dogs; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods*; D005609:Free Radicals / Q000528:radiation effects; D006801:Humans; D007908:Lens, Crystalline / Q000378:metabolism / Q000528:radiation effects*; D013113:Spin Labels; D014364:Tryptophan / Q000528:radiation effects; D014466:Ultraviolet Rays
D003497:Cyclic N-Oxides; D005609:Free Radicals; D013113:Spin Labels; C017245:5,5-dimethyl-1-pyrroline-1-oxide; D014364:Tryptophan; D003545:Cysteine