The cutting-grinding technique for histologic preparation of undecalcified bone and bone-anchored implants. Improvements in instrumentation and procedures.
An improved technique for histologic sectioning of hard tissues is described. Thin sections (5 to 15 microns) of undecalcified tissues such as bones with ceramic and metallic implants as well as teeth with enamel and fillings can be produced. Implant-tissue and hard tissue-soft tissue interfaces are well preserved. Because of the development of equipment and materials designed specifically for this technique, consistently good results are obtained. Most stains used in the paraffin technique may also be used in these sections.
Rohrer
M D
MD
College of Dentistry, University of Oklahoma Health Sciences Center, Oklahoma City.
Schubert
C C
CC
D000818:Animals; D001672:Biocompatible Materials; D001842:Bone and Bones / Q000033:anatomy & histology*; D002516:Ceramics; D015921:Dental Implants; D003793:Dental Restoration, Permanent; D017886:Durapatite; D016591:Histocytological Preparation Techniques / Q000295:instrumentation*; D006801:Humans; D006882:Hydroxyapatites; D016348:Osseointegration; D019736:Prostheses and Implants; D014070:Tooth / Q000033:anatomy & histology*
D001672:Biocompatible Materials; D015921:Dental Implants; D006882:Hydroxyapatites; D017886:Durapatite