[Regulation of purine biosynthesis. I. Isolation of add:: MudJ (lacZ, Kanr) insertions and genetic mapping].
Report here is the isolation of adenosine deaminase deficient mutants and genetic mapping. Engineering transposon MudJ (lacZ, Kanr) was used for mutagenesis and six add:: MudJ were obtained among 20,000 Kanr transductants. Adenosine deaminase activity of these mutants were assayed and all are negative. Cotransduction analysis of add::MudJ indicated that add is 70% linked to pmi(31') and 37% linked to zxx1900::Tn10d-tet insertion which is 10% linked to purR(30'). Three points cross showed that add is located between pmi and Tn10d-tet insertion. Therefore the gene order is purR-zxx1900::Tn10d-tet-add-pmi.
Wang
A
A
Institute of Microbiology, Academia Sinica, Beijing.
Chen
X
X
Dai
X
X
Tang
G
G
D000243:Adenosine Deaminase / Q000235:genetics; D002874:Chromosome Mapping; D004251:DNA Transposable Elements; D015964:Gene Expression Regulation, Bacterial; D016680:Genome, Bacterial; D011687:Purines / Q000378:metabolism*; D012486:Salmonella typhimurium / Q000235:genetics*; D014161:Transduction, Genetic
D004251:DNA Transposable Elements; D011687:Purines; D000243:Adenosine Deaminase