Collagenase activity in clinical isolates of Entamoeba histolytica maintained in xenic cultures.
Several isolates of pathogenic and non-pathogenic E. histolytica from xenic cultures were tested for their capacity to digest native type I collagen gels. The results demonstrate that the pathogenic isolate HM48:IMSS has a high collagenolytic activity. The non-pathogenic isolates, HM43:IMSS, HM44:IMSS and HM46:IMSS showed significantly lower collagenolytic activity. Six groups showing different degrees of collagenase activity or non-activity were separated from the non-pathogenic isolates by a Percoll gradient. The groups obtained from the pathogenic isolate HM48:IMSS always displayed enzymatic activity.
Magos
M A
MA
Department of Genetics and Molecular Biology, Centro de Investigación y de Estudios Avanzados del IPN, México, DF, México.
de la Torre
M
M
Muñoz
M L
ML
D000818:Animals; D002499:Centrifugation, Density Gradient; D003094:Collagen / Q000378:metabolism; D017364:Collagenases / Q000378:metabolism*; D004748:Entamoeba histolytica / Q000201:enzymology* / Q000254:growth & development / Q000472:pathogenicity; D010274:Parasitology / Q000379:methods*; D011205:Povidone; D015800:Protozoan Proteins / Q000378:metabolism*; D012822:Silicon Dioxide; D014774:Virulence
D015800:Protozoan Proteins; C016039:Percoll; D012822:Silicon Dioxide; D003094:Collagen; D017364:Collagenases; D011205:Povidone