[Effects of spermine on phosphorylation of phosphoinositide and 47 K protein in platelet membranes of pig].
In the presence of Mg2+ and exogenous phosphatidylinositol-4-phosphate, swine thrombocytic membranes were incubated with [gamma-32P]ATP at 30 degrees C for 3 min and the incorporations of 32P into phospholipids and proteins were measured. Spermine stimulated the formation of phosphatidylinositol-4,5-bisphosphate (optimal concentration 0.25 mmol.L-1) and inhibited the phosphorylation of 47 K protein (IC50 1 mmol.L-1). This helps to clarify the effect of spermine on cell growth and proliferation.
Ding
K H
KH
Department of Biochemistry, Zhanjiang Medical College, China.
Liang
N C
NC
D000818:Animals; D001792:Blood Platelets / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism*; D019269:Phosphatidylinositol 4,5-Diphosphate; D010716:Phosphatidylinositols / Q000096:biosynthesis / Q000378:metabolism*; D010766:Phosphorylation / Q000187:drug effects; D013096:Spermine / Q000494:pharmacology*; D013552:Swine
D008565:Membrane Proteins; D019269:Phosphatidylinositol 4,5-Diphosphate; D010716:Phosphatidylinositols; D013096:Spermine