title: SPT4, SPT5 and SPT6 interactions: effects on transcription and viability in Saccharomyces cerevisiae.
abstract: The SPT4, SPT5 and SPT6 genes of Saccharomyces cerevisiae were identified originally by mutations that suppress delta insertion mutations at HIS4 and LYS2. Subsequent analysis has demonstrated that spt4, spt5 and spt6 mutations confer similar pleiotropic phenotypes. They suppress delta insertion mutations by altering transcription and are believed to be required for normal transcription of several other loci. We have now analyzed interactions between SPT4, SPT5 and SPT6. First, the combination of mutations in any two of these three genes causes lethality in haploids. Second, some recessive mutations in different members of this set fail to complement each other. Third, mutations in all three genes alter transcription in similar ways. Finally, the results of coimmunoprecipitation experiments demonstrate that at least the SPT5 and SPT6 proteins interact physically. Taken together, these genetic and biochemical results indicate that SPT4, SPT5 and SPT6 function together in a transcriptional process that is essential for viability in yeast.
authors.affiliation: Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115.
authors.forename: M S
authors.initials: MS
authors.lastname: Swanson
authors.forename: F
authors.initials: F
authors.lastname: Winston
mesh_terms: D002868:Chromosomal Proteins, Non-Histone; D004251:DNA Transposable Elements; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D005798:Genes, Bacterial; D005804:Genes, Lethal; D016153:Genes, Suppressor; D005816:Genetic Complementation Test; D006026:Glycoside Hydrolases / Q000235:genetics; D056488:Histone Chaperones; D009154:Mutation; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins; D014158:Transcription, Genetic; D035602:Transcriptional Elongation Factors; D043324:beta-Fructofuranosidase
chemical_list: D002868:Chromosomal Proteins, Non-Histone; D004251:DNA Transposable Elements; D005656:Fungal Proteins; D056488:Histone Chaperones; D009687:Nuclear Proteins; D011993:Recombinant Fusion Proteins; C065456:SPT6 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D035602:Transcriptional Elongation Factors; C069940:SPT5 transcriptional elongation factor; D006026:Glycoside Hydrolases; D043324:beta-Fructofuranosidase