Increase in the expression of thyroid hormone receptors in porcine granulosa cells early in follicular maturation.
Thyroid hormone has been demonstrated to synergize with FSH to exert stimulatory effects on the differentiation of porcine granulosa cells. In order to further characterize the nature of thyroid hormone action on granulosa cells, the presence of triiodothyronine (T3) receptors in the nuclei of porcine granulosa cells was examined, and qualitatively and quantitatively compared during follicular maturation. Then, comparative abilities of granulosa cells from varying follicle stages to respond to T3 were assessed in terms of FSH-induced LH/hCG receptor formation and progesterone secretion. Furthermore, the expression of erb-A was analyzed using Northern blot hybridization of porcine granulosa cell RNA with a v-erb-A probe. Binding experiments with [125I]T3 showed that granulosa cell nuclei obtained from small follicles had a greater ability to bind [125I]T3 compared to those from large follicles. Scatchard analysis revealed the presence of nuclear T3 receptors with a single class of binding sites. There was little difference in the affinity of the T3 receptors during follicular maturation. By contrast, the number of the T3 receptors was higher in small follicle granulosa cells compared to that in large follicle granulosa cells. Thus, the increased T3 binding to small follicle granulosa cells relative to large follicle granulosa cells appears to be attributable to the increased number of the nuclear T3 receptors rather than to a change in the affinity. The magnitude of the stimulatory effects of T3 on granulosa cell functions was maximal in small follicle granulosa cells, but negligible in large follicle granulosa cells.(ABSTRACT TRUNCATED AT 250 WORDS)
Maruo
T
T
Department of Obstetrics and Gynecology, Kobe University School of Medicine, Japan.
Hiramatsu
S
S
Otani
T
T
Hayashi
M
M
Mochizuki
M
M
D000818:Animals; D015152:Blotting, Northern; D002467:Cell Nucleus / Q000378:metabolism / Q000648:ultrastructure; D002478:Cells, Cultured; D005260:Female; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D005498:Follicular Phase / Q000502:physiology*; D015870:Gene Expression / Q000502:physiology; D006107:Granulosa Cells / Q000737:chemistry* / Q000502:physiology / Q000648:ultrastructure; D011374:Progesterone / Q000097:blood; D011518:Proto-Oncogene Proteins / Q000032:analysis / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D011967:Receptors, Gonadotropin / Q000032:analysis / Q000378:metabolism; D011974:Receptors, LH / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D011988:Receptors, Thyroid Hormone / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D013552:Swine; D014158:Transcription, Genetic; D014284:Triiodothyronine / Q000378:metabolism
D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D011967:Receptors, Gonadotropin; D011974:Receptors, LH; D011988:Receptors, Thyroid Hormone; D014284:Triiodothyronine; D011374:Progesterone; D005640:Follicle Stimulating Hormone