title: Insertional inactivation of a gene which controls expression of vancomycin resistance on plasmid pHKK100.
abstract: Expression of inducible high level vancomycin resistance (Vmr) in enterococci appears to require other plasmid-encoded genes in addition to the previously described structural genes vanA and vanH. Tn917 mutagenesis was used to identify such a region in the Vmr plasmid pHKK100. Insertional inactivation of a 693-bp open reading frame upstream from vanH resulted in complete loss of Vmr. This putative 26,642-Da protein has been designated VanR.
authors.affiliation: Division of Infectious Diseases, Beth Israel Medical Center, Mount Sinai School of Medicine, New York, NY.
authors.forename: S
authors.initials: S
authors.lastname: Handwerger
authors.forename: L
authors.initials: L
authors.lastname: Discotto
authors.forename: J
authors.initials: J
authors.lastname: Thanassi
authors.forename: M J
authors.initials: MJ
authors.lastname: Pucci
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D004251:DNA Transposable Elements; D004352:Drug Resistance, Microbial / Q000235:genetics*; D016983:Enterococcus / Q000187:drug effects / Q000235:genetics*; D015870:Gene Expression; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D010641:Phenotype; D011815:R Factors / Q000235:genetics*; D014640:Vancomycin / Q000494:pharmacology*
chemical_list: D004251:DNA Transposable Elements; D014640:Vancomycin