Inhibitory effects of psychotomimetic sigma ligands on nicotine-induced K+ flux from differentiated PC12 cells.
In NGF-treated PC12 cells, nicotine-induced K+ release was measured with a K(+)-sensitive microelectrode. The K+ outflow via nicotinic ACh receptor cation channels was inhibited by various psychotomimetic sigma ligands in the sequence of PCP, dextromethorphan >> DTG, MK 801, (+)SKF10047 >> (+)3-PPP. The K+ release was not affected by the neuroleptic sigma ligand haloperidol nor by the calcium antagonist nifedipine. The results suggest that psychotomimetic sigma ligands inhibit nicotine-stimulated K+ flux by interacting with nicotinic, rather than via sigma 2 receptors.
Yamamoto
H
H
Department of Psychopharmacology, Tokyo Institute of Psychiatry, Japan.
Sagi
N
N
Yamamoto
T
T
Goji
Y
Y
Okuwa
M
M
Yoshii
M
M
Moroji
T
T
D000818:Animals; D006213:Hallucinogens / Q000494:pharmacology*; D008839:Microelectrodes; D009538:Nicotine / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D016716:PC12 Cells; D011188:Potassium / Q000378:metabolism*; D015221:Potassium Channels / Q000187:drug effects; D051381:Rats; D011950:Receptors, Cholinergic / Q000187:drug effects; D017465:Receptors, Opioid, delta / Q000187:drug effects*
D006213:Hallucinogens; D015221:Potassium Channels; D011950:Receptors, Cholinergic; D017465:Receptors, Opioid, delta; D009538:Nicotine; D011188:Potassium