title: Glucose-6-phosphatase in normal adult human intestinal mucosa.
abstract: 1. The existence of specific glucose-6-phosphatase activity in human intestinal mucosa has been somewhat controversial. 2. We have demonstrated the presence of low levels of specific glucose-6-phosphatase activity in normal human adult intestinal mucosa. Activity was found in oesophagus, stomach, duodenum and colon. 3. Immunoblot analysis using antibodies monospecific for the 36.5 kDa liver glucose-6-phosphatase catalytic subunit demonstrated that intestinal mucosa contains low levels of the glucose-6-phosphatase enzyme protein. 4. The low levels of activity together with problems of proteolysis make human intestinal biopsies unsuitable for use in the diagnosis of type 1 glycogen-storage disease.
authors.affiliation: Department of Medicine, Ninewells Hospital and Medical School, Dundee, U.K.
authors.forename: J
authors.initials: J
authors.lastname: Pears
authors.forename: R T
authors.initials: RT
authors.lastname: Jung
authors.forename: J
authors.initials: J
authors.lastname: Jankowski
authors.forename: I D
authors.initials: ID
authors.lastname: Waddell
authors.forename: A
authors.initials: A
authors.lastname: Burchell
mesh_terms: D000328:Adult; D004947:Esophagus / Q000201:enzymology; D005753:Gastric Mucosa / Q000201:enzymology; D005952:Glucose-6-Phosphatase / Q000378:metabolism*; D006801:Humans; D007413:Intestinal Mucosa / Q000201:enzymology*; D008861:Microsomes / Q000201:enzymology; D013379:Substrate Specificity
chemical_list: D005952:Glucose-6-Phosphatase