Release of choline by phospholipase D and a related phosphoric diester hydrolase in human erythrocytes. 1H spin-echo n.m.r. studies.
A previously detected phosphatidylcholine-specific phospholipase D from lysates of human red blood cells has been further characterized by 1H spin-echo n.m.r. spectroscopy. A second choline-releasing enzymic activity was observed after addition of glycerophosphocholine. Both of these phosphoric diester hydrolase activities were activated to different extents by different concentrations of calcium ions. Differences between the two activities were also observed on inhibition by barium and phosphate ions. These distinct, choline-yielding, reactions which occur in the cytoplasm of red blood cells may be involved in the regulation of the levels of membrane phosphatidylcholine.
Selle
H
H
Department of Biochemistry, University of Sydney, N.S.W., Australia.
Chapman
B E
BE
Kuchel
P W
PW
D001464:Barium / Q000494:pharmacology; D002118:Calcium / Q000494:pharmacology; D002794:Choline / Q000378:metabolism*; D003593:Cytoplasm / Q000201:enzymology; D004533:Egtazic Acid / Q000494:pharmacology; D004912:Erythrocytes / Q000201:enzymology* / Q000378:metabolism; D005997:Glycerylphosphorylcholine / Q000378:metabolism; D006801:Humans; D006867:Hydrolases / Q000097:blood*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D010710:Phosphates / Q000494:pharmacology; D010713:Phosphatidylcholines / Q000378:metabolism; D010739:Phospholipase D / Q000097:blood*; D010727:Phosphoric Diester Hydrolases / Q000097:blood*; D013057:Spectrum Analysis / Q000379:methods
D010710:Phosphates; D010713:Phosphatidylcholines; D001464:Barium; D004533:Egtazic Acid; D005997:Glycerylphosphorylcholine; D006867:Hydrolases; D010727:Phosphoric Diester Hydrolases; D010739:Phospholipase D; D002794:Choline; D002118:Calcium