Rapid DNA diagnosis of herpes simplex virus serotypes.
The presence of nucleotide sequences specific for each of herpes simplex virus (HSV) serotypes was demonstrated. These sequences were applied for dot DNA-DNA hybridization and for PCR for rapid DNA diagnosis of HSV infections. These sequences were found by molecular cloning of HSV-DNA fragments after digestion of DNA by KpnI enzyme. The type 1-specific sequence was found around the 5' end of BamHI B-fragment in the L region of type 1 DNA (corresponds to alpha gene 27, promoter-regulatory region) and the type 2-specific sequence was around the junction region of the L and S of type 2 DNA (corresponds to a' sequence). Both simple dot blot hybridization and PCR of HSV DNA's, employing these type-specific nucleotide sequences, were proven to be much more useful than immunofluorescence in terms of type-specific diagnosis of HSV infections.
Matsumoto
T
T
Department of Pathology, Research Institute for Microbial Diseases, Osaka University, Suita, Japan.
Yamada
O
O
Itagaki
A
A
Ishida
S
S
Kamahora
T
T
Kurimura
T
T
D001483:Base Sequence; D004279:DNA, Viral / Q000235:genetics*; D005455:Fluorescent Antibody Technique; D006561:Herpes Simplex / Q000175:diagnosis / Q000382:microbiology*; D006801:Humans; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D012703:Serotyping; D018139:Simplexvirus / Q000145:classification* / Q000235:genetics
D004279:DNA, Viral