Effect of intravenously infused eicosapentaenoic acid on the leukotriene generation in patients with active Crohn's disease.
Ten patients with active Crohn's disease who have been managed with parenteral-nutrition therapy were administered a lipid emulsion either with [containing 0.6 g eicosapentaenoic acid (EPA)] or without fish oil for 2 wk. We isolated polymorphonuclear leukocytes (PMNLs) from the patients before and after this treatment and measured the amounts of leukotriene B4 (LTB4) and leukotriene B5 (LTB5) generated by activated PMNLs by reversed-phase HPLC. The LTB5 generation in active Crohn's disease before this treatment was significantly lower than in healthy control subjects. The amount of LTB5 and the LTB5-LTB4 ratio increased significantly after fish-oil supplementation. The difference with LTB4 was not statistically significant. We have shown that daily intravenous administration of 0.6 g EPA influenced the generation of leukotrienes in active Crohn's disease even after short-term treatment. Further investigations are necessary to clarify the correlation between EPA and clinical improvement in Crohn's disease.
Ikehata
A
A
Third Department of Internal Medicine, Tohoku University School of Medicine, Sendai, Japan.
Hiwatashi
N
N
Kinouchi
Y
Y
Yamazaki
H
H
Kumagai
Y
Y
Ito
K
K
Kayaba
Y
Y
Toyota
T
T
D000328:Adult; D003424:Crohn Disease / Q000097:blood / Q000188:drug therapy*; D015118:Eicosapentaenoic Acid / Q000008:administration & dosage / Q000031:analogs & derivatives / Q000097:blood / Q000627:therapeutic use*; D005217:Fat Emulsions, Intravenous / Q000008:administration & dosage; D005260:Female; D005395:Fish Oils / Q000008:administration & dosage; D006801:Humans; D007262:Infusions, Intravenous; D007975:Leukotriene B4 / Q000097:blood / Q000031:analogs & derivatives; D015289:Leukotrienes / Q000097:blood*; D008297:Male; D009504:Neutrophils / Q000378:metabolism
D005217:Fat Emulsions, Intravenous; D005395:Fish Oils; D015289:Leukotrienes; D007975:Leukotriene B4; C040212:leukotriene B5; D015118:Eicosapentaenoic Acid