Effects of stress upon plasma estradiol and progesterone levels and the rate of oviductal embryo transport in the rat.
The possibility that changes in sex steroid levels associated with stress could alter the rate of oviductal embryo transport was investigated in the rat. To this end, the effect of cold-swimming and cold-restraint upon estradiol (E2) and progesterone (P) serum levels and embryo transport were assessed. Swimming in water at 16 degrees C for 10 min two or four times between 16:00 and 22:00 h on day 3 of pregnancy caused a modest acceleration of embryo transport that was not associated with decreased fertility. Restraint at 10 degrees C for 2 h between 13:00 and 15:00 h on the first 4 days of pregnancy did not affect embryo transport. Both stimuli increased corticosterone serum levels. Cold-swimming produced a severe hypothermia as compared to cold-restraint and increased serum E2, decreasing significantly the ratio P/E2. Cold-restraint increased the P/E2 ratio. When rats swam in cold water for 10 min twice and were rewarmed by immersion in water at 38 degrees C during 20 min, embryo transport was accelerated despite that no changes occurred in the blood levels of sex steroids. It is concluded that oviductal embryo transport is minimally affected by stress in the rat and that the effect of acute immersion may be independent of alterations in circulating sex steroid levels.
Cardenas
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Laboratorio de Endocrinología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Santiago.
D000818:Animals; D003080:Cold Temperature; D003345:Corticosterone / Q000097:blood; D004622:Embryo, Mammalian / Q000502:physiology*; D004958:Estradiol / Q000097:blood*; D005187:Fallopian Tubes / Q000502:physiology; D005260:Female; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000097:blood / Q000502:physiology*; D011374:Progesterone / Q000097:blood*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012149:Restraint, Physical; D013312:Stress, Physiological / Q000097:blood*; D013315:Stress, Psychological / Q000097:blood*
D011374:Progesterone; D004958:Estradiol; D003345:Corticosterone