[Interleukin 6 production and interleukin 6 mRNA gene expression by mouse glomerular mesangial cells].
To investigate the relationship between Interleukin 6(IL 6) and glomerular mesangial cells (MC), the MC of BALB/c mouse were cultured and verified subsequently by anti-keratin and antidesmin antibodies. The cultured MC were divided into two parts those with Mac-1 marker and those without by flow cytometry. The IL 6 activity of cultured supernatants of MC was measured using the IL 6-dependent cell line subclone (MH 60). The results show that the mouse MC can produce IL6 and the level of IL 6 produced by MC with Mac-1 marker was higher than that without Mac-1. The RNA from the mouse glomerular mesangial cells was hybridized in dot blots with a-32pdCTP labeled IL6 cDNA probe, the dot blot autoradiograph showed the expression of IL6 mRNA gene in mouse mesangial cells.
Chen
X M
XM
Department of Nephrology, PLA General Hospital, Beijing.
D000818:Animals; D002478:Cells, Cultured; D015342:DNA Probes; D005434:Flow Cytometry; D015870:Gene Expression; D005920:Glomerular Mesangium / Q000166:cytology / Q000378:metabolism*; D015850:Interleukin-6 / Q000096:biosynthesis / Q000235:genetics*; D051379:Mice; D008807:Mice, Inbred BALB C; D012333:RNA, Messenger / Q000032:analysis*
D015342:DNA Probes; D015850:Interleukin-6; D012333:RNA, Messenger