Induction of chemiluminescent emission in polymorphonuclear leucocytes stimulated by Sendai virus.
Sendai virus (SV) stimulates in vitro the chemiluminescent emission (CHL) of human polymorphonuclear leucocytes (LPMN). The kinetics of CHL produced by LPMN depends on the structure of stimulus, the intensity being dependent of the immunological status of the subject. CHL induced by Sendai virus is similar to that given by phorbol-myristate (PMA) as regards the induction of an early maximum. To obtain CHL significant emission, an optimum ratio between LPM concentration and Sendai virus dilution is required. The maximum effect is obtained in a large scale of dilution of Sendai virus ranging from 6,000 to 16,000 uHA.
Olinescu
R
R
Dept. of Radiobiology, Clinical Hospital Fundeni, Bucharest, Romania.
Săvoiu
D
D
Popescu
A
A
Zuivertz
A
A
Tomas
E
E
D002478:Cells, Cultured / Q000187:drug effects / Q000378:metabolism / Q000382:microbiology; D006801:Humans; D008163:Luminescent Measurements; D008213:Lymphocyte Activation / Q000187:drug effects; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism / Q000382:microbiology*; D010222:Parainfluenza Virus 1, Human / Q000276:immunology*; D013997:Time Factors; D015054:Zymosan / Q000494:pharmacology
D015054:Zymosan