title: The fast release of mucin secretion from human colonic cells induced by Entamoeba histolytica is dependent on contact and protein kinase C activation.
abstract: The mucus producing colonic cell line, LS174T, was used as a model to study E. histolytica-induced mucin secretion. E. histolytica trophozoites in contact with the mucus layer overlying the LS174T cells and in response to PMA, a protein kinase C activator, and Ca2+ ionophore A23187 which elevates intracellular Ca2+ ([Ca]i), caused a time-dependent (0.25-2.00 h) release of mucin. PKC inhibitors, H7 and staurosporine inhibited E. histolytica (37 and 75%) and PMA (46 and 100%)-induced mucin secretion, whereas in response to Ca2+ ionophore mucin secretion was augmented (56 and 17%). Both PMA and E. histolytica-induced the translocation of the PKC enzyme from the cytoplasm to the membrane fraction with increased enzyme activity. These results suggest that even though mucin secretion can be induced by PKC and Ca(2+)-dependent pathways, E. histolytica evokes the fast release of mucins by a PKC-dependent mechanism.
authors.affiliation: Institute of Parasitology of McGill University, Ste. Anne de Bellevue, Québec, Canada.
authors.forename: K
authors.initials: K
authors.lastname: Keller
authors.forename: M
authors.initials: M
authors.lastname: Olivier
authors.forename: K
authors.initials: K
authors.lastname: Chadee
mesh_terms: D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D000230:Adenocarcinoma / Q000473:pathology; D000470:Alkaloids / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000502:physiology*; D002240:Carbohydrate Sequence; D002448:Cell Adhesion; D003106:Colon / Q000378:metabolism*; D003110:Colonic Neoplasms / Q000473:pathology; D004748:Entamoeba histolytica / Q000502:physiology*; D004789:Enzyme Activation; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism*; D007546:Isoquinolines / Q000494:pharmacology; D037102:Lectins; D008562:Membrane Glycoproteins / Q000378:metabolism; D051379:Mice; D008819:Mice, Nude; D008969:Molecular Sequence Data; D009077:Mucins / Q000378:metabolism*; D009363:Neoplasm Proteins / Q000378:metabolism; D010879:Piperazines / Q000494:pharmacology; D011493:Protein Kinase C / Q000037:antagonists & inhibitors / Q000502:physiology*; D015800:Protozoan Proteins / Q000378:metabolism; D012636:Secretory Rate; D015398:Signal Transduction; D019311:Staurosporine; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014407:Tumor Cells, Cultured
chemical_list: D000470:Alkaloids; D007546:Isoquinolines; D037102:Lectins; D008562:Membrane Glycoproteins; D009077:Mucins; D009363:Neoplasm Proteins; D010879:Piperazines; D015800:Protozoan Proteins; C052452:galactose inhibitable adherence protein, Entamoeba histolytica; D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D011493:Protein Kinase C; D019311:Staurosporine; D013755:Tetradecanoylphorbol Acetate; D002118:Calcium