title: Immunogenicity of inactivated purified tissue culture vaccine against hepatitis A (HepAvac) assessed in laboratory rodents.
abstract: Immune response of laboratory rodents (guinea-pigs, CBA and Balb/c mice, Wistar and August rats) to inactivated hepatitis A vaccine was quantitatively assessed. Under comparable conditions of experiment, the mice showed the highest antibody titres and were capable of reacting to the lower doses of immunogen; meanwhile their individual variations in immune response were more pronounced; white rats were the least susceptible to the vaccine, demonstrating the minimal antibody formation; guinea-pigs produced antibody at intermediate levels but the antibody titres were the most homogeneous. The enhancing effect of aluminium hydroxide was observed in guinea-pigs examined at the late postimmunization stage. Differences in immunogenicity of three vaccine lots were essentially similar when these lots were tested as undiluted preparations in guinea-pigs and mice for mean antibody titres and in mice for 50% immune response using serial dilutions of vaccine. All three tests could be routinely employed for vaccine immunogenicity control.
authors.affiliation: Institute of Poliomyelitis and Virus Encephalitides, Russian Academy of Medical Sciences, Moscow.
authors.forename: L B
authors.initials: LB
authors.lastname: Elbert
authors.forename: E A
authors.initials: EA
authors.lastname: Lisitzina
authors.forename: Y Y
authors.initials: YY
authors.lastname: Kusov
authors.forename: M S
authors.initials: MS
authors.lastname: Balayan
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis*; D004306:Dose-Response Relationship, Immunologic; D006168:Guinea Pigs; D022362:Hepatitis A Vaccines; D006507:Hepatovirus / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008808:Mice, Inbred CBA; D051381:Rats; D017208:Rats, Wistar; D013045:Species Specificity; D015164:Vaccines, Inactivated / Q000276:immunology; D014761:Viral Hepatitis Vaccines / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D022362:Hepatitis A Vaccines; D015164:Vaccines, Inactivated; D014761:Viral Hepatitis Vaccines