Brain oxygenation state: preparation of isolated perfused rat brain and near-infrared spectrophotometry.
The method of blood-free isolated perfused rat brain was described, of which EEG was within the normal range. The simultaneous measurement of heme a+a3 and copper in cytochrome oxidase could be performed under the various conditions such as graded hypoxia and seizure. The relationship between these two chromophores in the brain was same to that of isolated mitochondria.
Inagaki
M
M
Biophysics Division, Hokkaido University, Sapporo, Japan.
Tamura
M
M
D000818:Animals; D001921:Brain / Q000378:metabolism*; D003300:Copper / Q000378:metabolism; D003576:Electron Transport Complex IV / Q000378:metabolism; D005069:Evaluation Studies as Topic; D006418:Heme / Q000031:analogs & derivatives / Q000378:metabolism; D002534:Hypoxia, Brain / Q000378:metabolism; D066298:In Vitro Techniques; D010084:Oxidation-Reduction; D010101:Oxygen Consumption; D010477:Perfusion; D051381:Rats; D012640:Seizures / Q000378:metabolism; D013055:Spectrophotometry, Infrared / Q000295:instrumentation
C027728:heme a; D006418:Heme; D003300:Copper; D003576:Electron Transport Complex IV