Transcription factor activation and functional stimulation of human monocytes.
Activation of expression of genes encoding transcription factors: c-fos and c-jun and formation of AP1 transcriptional complex in human monocytes was investigated. It was found that lipopolysaccharide induced strongly both c-fos and c-jun expression as well as AP1 formation. Interferon gamma activated strongly c-fos and weakly c-jun and AP1. Tumor necrosis factor induced slightly c-fos and had almost no effect on c-jun and AP1. The data suggest that differences in functional responses elicited in monocytes by all three factors may be dependent on different routes on nuclear signalling employed by the factors.
Kamińska
B
B
Nencki Institute of Experimental Biology, Warsaw, Poland.
Kaczmarek
L
L
Malaguarnera
L
L
Arcidiacono
A
A
Messina
L
L
Spampinato
G
G
Messina
A
A
D005786:Gene Expression Regulation / Q000187:drug effects; D016762:Genes, fos / Q000187:drug effects*; D016758:Genes, jun / Q000187:drug effects*; D006801:Humans; D015151:Immunoblotting; D007371:Interferon-gamma / Q000494:pharmacology*; D008070:Lipopolysaccharides; D009000:Monocytes / Q000187:drug effects* / Q000502:physiology; D016755:Proto-Oncogene Proteins c-jun / Q000032:analysis; D015398:Signal Transduction; D013481:Superoxides / Q000032:analysis; D015533:Transcriptional Activation / Q000187:drug effects*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
D008070:Lipopolysaccharides; D016755:Proto-Oncogene Proteins c-jun; D014409:Tumor Necrosis Factor-alpha; D013481:Superoxides; D007371:Interferon-gamma