Typing of methicillin-resistant Staphylococcus aureus with IS256.
A simple one-step procedure is described for specifically amplifying and labelling insertion element IS256 which is associated with the gentamicin-resistance transposon Tn4001. The product has been used to probe DNA digests of methicillin-resistant Staphylococcus aureus. The resulting restriction fragment length polymorphisms were found to be able to distinguish isolates which were indistinguishable by other typing methods. The probe also hybridised with methicillin-resistant Staphylococcus aureus which were isolated before the emergence of gentamicin resistance, demonstrating its usefulness in typing species other than those that are gentamicin-resistant.
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School of Biomedical Sciences, Curtin University of Technology, Bentley, W.A., Australia.
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D015373:Bacterial Typing Techniques; D001483:Base Sequence; D015342:DNA Probes; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000235:genetics; D006801:Humans; D016106:Methicillin Resistance / Q000235:genetics; D008969:Molecular Sequence Data; D010957:Plasmids; D016133:Polymerase Chain Reaction; D013211:Staphylococcus aureus / Q000145:classification* / Q000187:drug effects / Q000235:genetics*
D015342:DNA Probes; D004251:DNA Transposable Elements; D004269:DNA, Bacterial