title: Freeze-dried, cross-linked bovine type I collagen: analysis of properties.
abstract: This study was undertaken to assess the physical and biological properties of freeze-dried cross-linked bovine type I collagen and to assess its potential for use in the guided tissue regeneration method of treatment of periodontal disease in human adult subjects. The modulus of elasticity, swelling ratio, and biodegradation rate were investigated. The collagen sponge was implanted subdermally into Sprague-Dawley rats and a histological study carried out at 2, 7, 21, 35, and 49 days post implantation. Growth of human gingival and periodontal ligament derived fibroblasts on collagen sponge was assessed, as well as the effect of bovine collagen supernatants upon gingival and periodontal fibroblast cultures. The physical properties of the collagen sponge were consistent with good handling qualities and, therefore, it was appropriate for use at a surgical site. The histological study demonstrated a reduction in thickness of the collagen at 21 days; at 35 days there was a hazy appearance of the collagen remnants; and at 49 days the graft material had been completely replaced with fibrous tissues. The in vitro response of human gingival and periodontal fibroblasts to bovine collagen showed that, after 21 days, confluent fibroblast growth was observed around and underneath the sponge. The effect of bovine collagen supernatants upon fibroblasts demonstrated an apparent proliferative effect of the supernatant with both gingival and periodontal ligament fibroblasts. However, the non-parametric Friedman test revealed no significant differences between dilutions or time points. The overall findings provide encouraging evidence of the safety of freeze-dried cross-linked bovine collagen sponge in the surgical treatment of periodontal disease.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Periodontology, University of Wales College of Medicine, Heath Park, Cardiff, UK.
authors.forename: P R
authors.initials: PR
authors.lastname: Hyder
authors.forename: P
authors.initials: P
authors.lastname: Dowell
authors.forename: G
authors.initials: G
authors.lastname: Singh
authors.forename: A E
authors.initials: AE
authors.lastname: Dolby
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D001673:Biodegradation, Environmental; D002417:Cattle; D002478:Cells, Cultured; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D003094:Collagen / Q000737:chemistry* / Q000378:metabolism; D003238:Connective Tissue / Q000473:pathology; D003470:Culture Media; D004548:Elasticity; D005347:Fibroblasts / Q000473:pathology; D005612:Freeze Drying; D005881:Gingiva / Q000473:pathology; D006801:Humans; D003012:Microbial Collagenase / Q000378:metabolism; D010513:Periodontal Ligament / Q000473:pathology; D019736:Prostheses and Implants; D051381:Rats; D011919:Rats, Inbred Strains; D012867:Skin / Q000473:pathology; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D003470:Culture Media; D003094:Collagen; D003012:Microbial Collagenase