title: Regional flow during experimental hemorrhage and crystalloid resuscitation: persistence of low flow to the splanchnic organs.
abstract: BACKGROUND AND METHODS. Rapid changes in cardiac output (CO) and organ perfusion occur with hemorrhagic shock and fluid resuscitation. To assess regional alterations of flow, 40 Sprague-Dawley male rats were subjected to hemorrhagic shock and crystalloid resuscitation under halothane anesthesia. Polyethylene microspheres were injected before and after hemorrhage and after resuscitation. At sacrifice, brain, lungs, heart, liver, intestine, spleen and kidneys were harvested, weighed and radioactivity counted. Changes in mean arterial pressure, oxygen consumption, organ flow and CO were also measured.
  
  
  RESULTS
  Cardiac output decreased during hemorrhage (P less than 0.01), it increased with resuscitation but did not return to baseline even with infusion of fluid volumes of three times the blood loss. Flow decreased during hemorrhage in all organs, but the difference was not statistically significant in the liver (P greater than 0.05), since a larger percentage of CO was maintained as hepatic perfusion. During resuscitation, flow to brain and kidneys increased over the percentage values expected by increased CO (P less than 0.01), but flow to the liver did not increase significantly. Flow to small bowel remained depressed (P less than 0.005).
  
  
  CONCLUSIONS
  Following hemorrhage there is hypoperfusion of all splanchnic organs; however, flow to the liver decreases least. Crystalloid resuscitation in our model failed to return CO to baseline. Blood supply to intestine remained depressed in disproportion to CO both after hemorrhage and resuscitation and hepatic blood flow remained decreased after resuscitation.
authors.affiliation: Department of Surgery, University of California, Irvine.
authors.forename: G
authors.initials: G
authors.lastname: Scannell
authors.forename: L
authors.initials: L
authors.lastname: Clark
authors.forename: K
authors.initials: K
authors.lastname: Waxman
mesh_terms: D000818:Animals; D002302:Cardiac Output / Q000502:physiology; D005440:Fluid Therapy; D007552:Isotonic Solutions; D008102:Liver Circulation / Q000502:physiology; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012151:Resuscitation / Q000379:methods*; D000077325:Ringer's Lactate; D012771:Shock, Hemorrhagic / Q000628:therapy*; D013152:Splanchnic Circulation / Q000502:physiology*
chemical_list: D007552:Isotonic Solutions; D000077325:Ringer's Lactate