title: Linear, non-mitochondrial plasmids of Alternaria alternata.
abstract: Three plasmids, with sizes of 7.0 kbp, 6.8 kbp, and 5.0 kbp and designated pAal-1, pAal-2 and pAal-3 respectively, have been found in a tentoxin-producing isolate of Alternaria alternata. Exonuclease digestions show these plasmids to be linear with blocked 5' ends. Plasmid pAal-1 does not hybridize to nuclear DNA, mitochondrial DNA, or double-stranded RNA from a mycovirus found in the isolate, but does hybridize weakly to a series of linear DNAs which are not visible on gels and may include pAal-2 and pAal-3. Cellular fractionation shows that, unlike other linear fungal plasmids, these plasmids are not localized in the mitochondria. Plasmids have not been found in other tentoxin-producing isolates and there is no evidence that these plasmids have any effect on the production of tentoxin.
authors.affiliation: United States Department of Agriculture, ARS Southern Regional Research Center, New Orleans, LA 70179.
authors.forename: H S
authors.initials: HS
authors.lastname: Shepherd
mesh_terms: D000528:Alternaria / Q000235:genetics*; D004262:DNA Restriction Enzymes / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism; D010957:Plasmids
chemical_list: D004262:DNA Restriction Enzymes