Transcriptional activity of HIV-1 and HHV-6 in retinal lesions from AIDS patients.
This study determined the frequency of multiple viral (HIV-1, HHV-6, and CMV) infections in 26 retinas from 16 AIDS patients. Of the 12 retinas of 26 that tested positive for HIV-1 DNA sequences, seven also were positive for HHV-6 DNA sequences. Four of these seven retinas were culture positive for HIV-1 and two of the four contained CMV DNA sequences and antigens. Using RNA probes, HIV-1 and HHV-6 transcriptional activity was demonstrated in two of the four HIV-1 culture positive retinas. These retinas also contained CMV DNA sequences and antigens. The results demonstrate that more than 35% of AIDS patients suffer from at least two simultaneous viral infections and 15% suffer from three viral infections. The presence of transcriptional activity of HIV-1 and HHV-6 suggests an active infection.
Qavi
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Division of Molecular Virology, Baylor College of Medicine, Houston, Texas 77030.
Green
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MT
SeGall
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GK
Lewis
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DE
Hollinger
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FB
D000163:Acquired Immunodeficiency Syndrome / Q000382:microbiology / Q000473:pathology; D000914:Antibodies, Viral / Q000032:analysis; D001483:Base Sequence; D003587:Cytomegalovirus / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification / Q000648:ultrastructure; D003586:Cytomegalovirus Infections / Q000382:microbiology / Q000473:pathology; D004279:DNA, Viral / Q000235:genetics; D015828:Eye Infections, Viral / Q000382:microbiology*; D015967:Gene Expression Regulation, Viral; D015658:HIV Infections / Q000382:microbiology*; D015497:HIV-1 / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification / Q000648:ultrastructure; D006566:Herpesviridae Infections / Q000382:microbiology*; D015654:Herpesvirus 6, Human / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification / Q000648:ultrastructure; D006801:Humans; D008969:Molecular Sequence Data; D015347:RNA Probes; D012164:Retinal Diseases / Q000382:microbiology* / Q000473:pathology
D000914:Antibodies, Viral; D004279:DNA, Viral; D015347:RNA Probes