title: The induction of IL-6 and gelatinase B by IL-1 in mouse cell lines transformed with bovine papillomavirus: decreased production in tumorigenic cells.
abstract: Six cell lines, that were cloned from murine C127 cells infected by bovine papillomavirus type 1 (BPV1), were found to differ in the degree of transformation in vitro and of tumorigenicity in vivo. In these cell lines the degree of tumorigenicity was inversely correlated with IL-6 induction by IL-1 beta. Whereas the parental C127 cell line produced 15-30 U/ml of IL-6 spontaneously, none of the transformed cell lines produced significant levels of IL-6 constitutively. On induction by human IL-1 beta the parental C127 cell line produced up to 300 U/ml of IL-6, whereas the fully transformed ID14 cell line failed to produce any. The less transformed cell lines produced lower yields of IL-1 beta-induced IL-6, dependent on their degrees of transformation and tumorigenicity. Gelatinase B (96 kDa), a matrix metalloproteinase inducible by IL-1 beta, was dose-dependently regulated in the parental C127 cell line and in the weakly transformed cell line Tlc. These data suggest that transformation processes by BPV1 generally impair IL-1-regulated gene transcription. This impairment seems not to be located at the IL-1 beta receptor level, since in all the cell lines studied the numbers and affinities of the IL-1 beta binding sites were found to be comparable. This impairment seems not to be mediated by transformation-induced inactivation of the protein kinase C pathway since phorbol 12-myristate 13-acetate (PMA) induced IL-6 production equally well in all C127 cell-derived clones. It is suggested that BPV1 transformation can change the expression of host genes that might play a functional role in tumor immune surveillance and tumorigenicity in vivo.
authors.affiliation: Rega Institute for Medical Research, University of Leuven, Belgium.
authors.forename: A
authors.initials: A
authors.lastname: Fuse
authors.forename: B
authors.initials: B
authors.lastname: Simizu
authors.forename: M
authors.initials: M
authors.lastname: Van Ranst
authors.forename: G
authors.initials: G
authors.lastname: Opdenakker
authors.forename: J
authors.initials: J
authors.lastname: Van Damme
mesh_terms: D000818:Animals; D052688:Bovine papillomavirus 1; D002461:Cell Line, Transformed; D002471:Cell Transformation, Neoplastic / Q000378:metabolism*; D002472:Cell Transformation, Viral / Q000502:physiology*; D017364:Collagenases / Q000096:biosynthesis*; D004790:Enzyme Induction / Q000502:physiology; D007375:Interleukin-1 / Q000378:metabolism / Q000502:physiology*; D015850:Interleukin-6 / Q000096:biosynthesis*; D020780:Matrix Metalloproteinase 9; D051379:Mice; D008819:Mice, Nude; D011485:Protein Binding
chemical_list: D007375:Interleukin-1; D015850:Interleukin-6; D017364:Collagenases; D020780:Matrix Metalloproteinase 9