Effects of intracerebroventricular captopril on angiotensin-converting enzyme and angiotensinogen mRNA levels in rat brain.
The effects of intracerebroventricular (i.c.v.) infusion of the angiotensin-converting enzyme (ACE) inhibitor captopril on angiotensin-induced drinking, brain ACE activity, and ACE and angiotensinogen (A-ogen) mRNA levels were examined. I.c.v. infusion of captopril at a rate of 1 microgram/microliter per h for 7 days resulted in a 60% reduction in brain ACE activity and an 80% reduction in the drinking response to i.c.v. angiotensin I. Quantitative solution hybridization experiments indicated that brain ACE mRNA levels were decreased by 40%, whereas brain A-ogen mRNA levels were unchanged. These results suggest that ACE and A-ogen mRNA levels are regulated differently in the brain than in the peripheral renin-angiotensin system.
Ye
M Q
MQ
Department of Pharmacology, Mount Sinai School of Medicine, City University of New York, NY 10029.
Yuan
L X
LX
Healy
D P
DP
D000808:Angiotensinogen / Q000235:genetics*; D000809:Angiotensins / Q000008:administration & dosage / Q000494:pharmacology; D000818:Animals; D001923:Brain Chemistry / Q000187:drug effects*; D002216:Captopril / Q000008:administration & dosage / Q000494:pharmacology*; D004326:Drinking / Q000187:drug effects; D007276:Injections, Intraventricular; D008297:Male; D007703:Peptidyl-Dipeptidase A / Q000032:analysis*; D012333:RNA, Messenger / Q000032:analysis*; D051381:Rats; D017207:Rats, Sprague-Dawley
D000809:Angiotensins; D012333:RNA, Messenger; D000808:Angiotensinogen; D002216:Captopril; D007703:Peptidyl-Dipeptidase A