Short sequence in L1 region of human papillomaviruses correlates with clinical pictures and grouping by cross-hybridization.
A consensus primer-mediated polymerase chain reaction devised to amplify a short sequence in L1 region (L1-PCR) efficiently detected genital human papillomaviruses (HPVs) in clinical materials. Nucleotide sequencing of the amplified fragment showed that L1-PCR is also applicable to hitherto unsequenced HPVs. By comparing the amplified 210 nucleotides HPVs were classified into six groups, which are consistent with clinical pictures and a grouping based on cross-hybridization under the stringent condition.
Iwamoto
A
A
Department of Bacteriology, Faculty of Medicine, University of Tokyo.
Yoshikawa
H
H
Kitagawa
K
K
Igarashi
H
H
Kawana
T
T
Yoshikura
H
H
D001483:Base Sequence; D002584:Cervix Uteri / Q000382:microbiology*; D005260:Female; D006801:Humans; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D027383:Papillomaviridae / Q000235:genetics* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D002578:Uterine Cervical Dysplasia / Q000382:microbiology*; D002583:Uterine Cervical Neoplasms / Q000382:microbiology*
D009838:Oligodeoxyribonucleotides