A P element chimera containing captured genomic sequences was recovered at the vestigial locus in Drosophila following targeted transposition.
A P element carrying the Dopa decarboxylase gene, P[Ddc], was targeted into vg21, a cryptic P element induced mutant allele of the vestigial (vg) locus. The resulting allele, vg28w, contained the expected P[Ddc] plus an additional 9.5 kb of DNA, captured from elsewhere on chromosome II. Reversion of the vg28w mutant allele demonstrated that the entire insert can excise but cannot reinsert at an appreciable frequency. We explain the targeted transposition as the repair of a double stranded gap, created by the excision of the P element at vg21, and suggest that the formation of chimeric elements may be an important component of P element dependent genomic instability.
Heslip
T R
TR
Department of Genetics, University of Alberta, Edmonton, Canada.
Williams
J A
JA
Bell
J B
JB
Hodgetts
R B
RB
D000483:Alleles; D000818:Animals; D001483:Base Sequence; D002678:Chimera; D004247:DNA / Q000235:genetics; D004252:DNA Mutational Analysis; D004251:DNA Transposable Elements; D004296:Dopa Decarboxylase / Q000235:genetics; D004331:Drosophila melanogaster / Q000201:enzymology / Q000235:genetics*; D005260:Female; D008297:Male; D008969:Molecular Sequence Data; D015183:Restriction Mapping
D004251:DNA Transposable Elements; D004247:DNA; D004296:Dopa Decarboxylase