title: Influence of Epstein-Barr virus genomes on patient survival in Hodgkin's disease.
abstract: In previous studies, Epstein-Barr virus was considered a possible etiologic factor in Hodgkin's disease. Two hundred twenty-nine cases of Hodgkin's disease were investigated for the presence of Epstein-Barr virus DNA using the polymerase chain reaction technique on formalin-fixed, paraffin-embedded lymph node tissue to clarify the clinical importance of the incidence of this genome. In 42 cases (18.3%), genomic DNA was not amplifiable. The remaining 187 cases included the following subtypes: lymphocyte-predominant type (n = 13), nodular sclerosis type (n = 98), mixed cellularity type (n = 68), and lymphocyte-depleted type (n = 8). Sixty-six cases (35.2%) were positive for Epstein-Barr virus DNA. In the statistical analysis of available follow-up data from 130 patients, no influence of a positive Epstein-Barr virus DNA finding on length of survival time was revealed. This was true within the cohort of all patients and within the histologically defined subtypes of Hodgkin's disease. In this investigation, detection of Epstein-Barr virus DNA by polymerase chain reaction showed no prognostic relevance for patients with Hodgkin's disease.
authors.affiliation: Institute of Pathology, School of Medicine, Technical University of Munich, Federal Republic of Germany.
authors.forename: C
authors.initials: C
authors.lastname: Fellbaum
authors.forename: M L
authors.initials: ML
authors.lastname: Hansmann
authors.forename: H
authors.initials: H
authors.lastname: Niedermeyer
authors.forename: I
authors.initials: I
authors.lastname: Kraus
authors.forename: M J
authors.initials: MJ
authors.lastname: Alavaikko
authors.forename: G
authors.initials: G
authors.lastname: Blanco
authors.forename: R
authors.initials: R
authors.lastname: Aine
authors.forename: R
authors.initials: R
authors.lastname: Busch
authors.forename: B
authors.initials: B
authors.lastname: Pütz
authors.forename: R
authors.initials: R
authors.lastname: Fischer
mesh_terms: D000704:Analysis of Variance; D001483:Base Sequence; D004279:DNA, Viral / Q000032:analysis; D005500:Follow-Up Studies; D004854:Herpesvirus 4, Human / Q000235:genetics* / Q000302:isolation & purification; D006689:Hodgkin Disease / Q000382:microbiology* / Q000401:mortality; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D016019:Survival Analysis
chemical_list: D004279:DNA, Viral