title: Dolichol and polyprenol kinase activities in microsomes from etiolated rye seedlings.
abstract: Dolichol kinase activity in microsomes from etiolated rye seedlings had a pH optimum at 8 with a shoulder at pH 6.5. Triton X-100 (0.4%) was required for optimum activity. Exogenous divalent cations did not enhance activity, although Mg+2 was added routinely. Rye microsomes were found to contain dolichol and polyprenol in a ratio of 3 to 2. Rye, soybean embryo, and rat liver microsomes catalyzed the synthesis of 78, 52, and 516 nmol [14C]dolichyl phosphate/(mg microsomal protein.h) compared with 21, 22, and 49 nmol [3H]polyprenyl phosphate/(mg microsomal protein.h), respectively. It is clear that microsomes from plant systems can catalyze the phosphorylation of polyprenol better than rat liver when compared with their abilities to catalyze the phosphorylation of dolichol. It is not known whether one or more kinases is responsible for catalyzing the phosphorylation of these two closely related groups of compounds.
authors.affiliation: Department of Biochemistry, University of Western Ontario, London, Canada.
authors.forename: R T
authors.initials: RT
authors.lastname: Rymerson
authors.forename: K K
authors.initials: KK
authors.lastname: Carroll
authors.forename: J W
authors.initials: JW
authors.lastname: Rip
mesh_terms: D000818:Animals; D002412:Cations / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D003902:Detergents / Q000494:pharmacology; D004288:Dolichol Phosphates / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008861:Microsomes / Q000201:enzymology*; D010766:Phosphorylation; D010770:Phosphotransferases / Q000378:metabolism*; D017853:Phosphotransferases (Alcohol Group Acceptor); D010940:Plant Proteins / Q000378:metabolism*; D051381:Rats; D012434:Secale / Q000201:enzymology*; D013025:Glycine max / Q000201:enzymology; D013045:Species Specificity; D013379:Substrate Specificity
chemical_list: D002412:Cations; D003902:Detergents; D004288:Dolichol Phosphates; D010940:Plant Proteins; D010770:Phosphotransferases; D017853:Phosphotransferases (Alcohol Group Acceptor); C022426:polyprenol kinase; C020087:dolichol kinase