Analysis of ras oncogene mutations in human squamous cell carcinoma of the head and neck.
The presence of proto-oncogene mutations at codons 12, 13 and 61 of the Ha-, Ki-, and N-ras in primary head and neck squamous cell carcinoma are analysed in this study. Oncogene ras-specific sequences were amplified by the polymerase chain reaction and probed with mutation specific oligonucleotide probes. Mutations were detected in 8 of 22 samples (36.3%). No mutations were detected on patients' peripheral blood DNA. We found that histologically and clinically, squamous cell carcinomas with or without a ras mutation do not differ significantly from each other.
Núñez
F
F
Department of Otolaryngology, Hospital Central de Asturias, Oviedo, Spain.
Domínguez
O
O
Coto
E
E
Suárez-Nieto
C
C
Pérez
P
P
López-Larrea
C
C
D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D016009:Chi-Square Distribution; D004247:DNA / Q000235:genetics; D011905:Genes, ras / Q000235:genetics*; D006258:Head and Neck Neoplasms / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D006801:Humans; D009154:Mutation / Q000235:genetics*; D009367:Neoplasm Staging; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction; D000090063:Proto-Oncogene Mas; D013030:Spain / Q000453:epidemiology; D016019:Survival Analysis
C000715470:MAS1 protein, human; D015345:Oligonucleotide Probes; D000090063:Proto-Oncogene Mas; D004247:DNA