[An experimental study on the mechanism of impairment of cell membrane during hemorrhagic shock in dogs].
Dogs were bled and maintained at an arterial pressure of 6.7 kPa for 3 hours. During hemorrhagic shock, red blood cell and serum were assayed for Na+, K+ and Ca++ ion level. RBC and hepatic tissue were together assayed for adenosine triphosphatase (ATPase) activities, free radicals. Results showed that: (1) at 15 minutes after hemorrhagic shock. ATPase activities in RBC and hepatic tissue was significantly elevated. While ratio of sodium and potassium (Na+/K+) in RBC was showed no significant difference; (2) ATPase activities in RBC and liver were decreased, Na+/K+ in RBC was elevated at first after hemorrhagic shock; (3) the signals of free radicals were increased during hemorrhagic shock. The signals of free radicals and ATPase activities in cell membrane was negative correlated. While signals of free radicals and Na+/K+ in RBC was positive correlated. Based on these findings, it is proposed that: free radicals after hemorrhagic shock is an important factor responsible for decrease of ATPase activities and dysfunction of ions active transport in cell membrane.
Wang
J Y
JY
First Affiliated Hospital, Fourth Military medical college, Xian.
Wang
H
H
Pang
Z Q
ZQ
D000818:Animals; D000252:Calcium-Transporting ATPases / Q000378:metabolism / Q000502:physiology*; D002462:Cell Membrane / Q000502:physiology; D004285:Dogs; D004910:Erythrocyte Membrane / Q000502:physiology*; D005260:Female; D005609:Free Radicals; D008099:Liver / Q000201:enzymology; D008297:Male; D012771:Shock, Hemorrhagic / Q000097:blood / Q000201:enzymology / Q000503:physiopathology*; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism / Q000502:physiology*
D005609:Free Radicals; D000252:Calcium-Transporting ATPases; D000254:Sodium-Potassium-Exchanging ATPase