Nucleotide sequence of a carboxyphosphonoenolpyruvate phosphonomutase gene isolated from a bialaphos-producing organism, Streptomyces hygroscopicus, and its expression in Streptomyces lividans.
The carboxyphosphonoenolpyruvate (CPEP) phosphonomutase gene of bialaphos-producing Streptomyces hygroscopicus, which encodes a C-P bond forming enzyme was cloned into Streptomyces lividans and sequenced. The amino acid composition of the protein coded in an open reading frame of 295 codons and its calculated molecular mass, 32,800 Da, coincided well with those of the purified enzyme. Introduction of the CPEP phosphonomutase gene, the expression of which is controlled by the promoter of the aph gene, into S. lividans resulted in the production of this enzyme at a level almost equivalent to that in the parent strain.
Hidaka
T
T
Institute of Applied Microbiology, University of Tokyo, Japan.
Hidaka
M
M
Uozumi
T
T
Seto
H
H
D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000378:metabolism; D001483:Base Sequence; D003001:Cloning, Molecular; D015870:Gene Expression / Q000235:genetics; D008969:Molecular Sequence Data; D009943:Organophosphorus Compounds / Q000378:metabolism; D010770:Phosphotransferases / Q000737:chemistry / Q000235:genetics*; D017875:Phosphotransferases (Phosphomutases); D011401:Promoter Regions, Genetic / Q000235:genetics; D015183:Restriction Mapping; D013302:Streptomyces / Q000201:enzymology* / Q000235:genetics
D000900:Anti-Bacterial Agents; D009943:Organophosphorus Compounds; C003122:bialaphos; D010770:Phosphotransferases; D017875:Phosphotransferases (Phosphomutases); C064363:carboxyphosphonoenolpyruvate phosphonomutase