A single human colonic adenoma cell line can be converted in vitro to both a colorectal adenocarcinoma and a mucinous carcinoma.
In a previous study, using a chemical carcinogen, we converted in vitro a non-tumorigenic cell line derived from a human colorectal diploid adenoma, designated PC/AA, into a tumorigenic cell line which, when inoculated into athymic nude mice, produced progressively growing adenocarcinomas. We now report that continuous in vitro passage of the PC/AA adenoma cell line resulted in its spontaneous transformation to a mucinous carcinoma with a modal karyotype of 51, XY, +i(Iq), +8, +9, +13, +i(13q), -21, +mar. These studies show that a single adenoma can be converted along 2 independent pathways, giving rise to either a mucinous carcinoma or an adenocarcinoma, and provide further experimental evidence for the adenoma-carcinoma sequence. Cytogenetic changes which occur along both pathways to tumorigenicity include abnormalities of chromosome I and multiple copies of chromosome 13. These abnormalities may be important in tumour development and progression in colorectal carcinogenesis.
Paraskeva
C
C
Department of Pathology and Microbiology, University of Bristol, School of Medical Sciences, UK.
Hague
A
A
Rooney
N
N
Williams
A C
AC
Harper
S J
SJ
Hanlon
K A
KA
Atkinson
R J
RJ
Corfield
A P
AP
D000230:Adenocarcinoma / Q000473:pathology* / Q000648:ultrastructure; D002288:Adenocarcinoma, Mucinous / Q000473:pathology* / Q000648:ultrastructure; D000236:Adenoma / Q000473:pathology* / Q000648:ultrastructure; D000818:Animals; D002460:Cell Line; D002869:Chromosome Aberrations; D003110:Colonic Neoplasms / Q000473:pathology* / Q000648:ultrastructure; D015179:Colorectal Neoplasms / Q000473:pathology* / Q000648:ultrastructure; D006801:Humans; D066298:In Vitro Techniques; D007621:Karyotyping; D051379:Mice; D008819:Mice, Nude