Selective inhibition of proliferation in v-abl- and bcr-abl-transformed cells by a nucleoside analog.
The nucleoside analog acyclovir (9-[2-hydroxy-ethoxy)methyl]guanine or acycloguanosine; ACV) inhibited the in vitro transformation of NIH 3T3 cells by Abelson murine leukemia virus and the proliferation of abl- and bcr-abl-transformed hemopoietic murine cell lines. This effect is selective since ACV at the same concentration had no effect on the src and Ha-ras transformation of NIH 3T3 cells or on the proliferation of hemopoietic cells transformed by those oncogenes. The inhibitory effect on proliferation of abl-transformed cells correlated with the extent of ACV triphosphate formation and incorporation into cellular DNA that was greater than that in normal or other oncogene-transformed cells. The increased ACV triphosphate formation might be due to a higher level of 5'-nucleotidase, the enzyme responsible for trace levels of ACV phosphorylation in uninfected cells.
Cioé
L
L
Wistar Institute, Philadelphia, Pennsylvania 19104-4268.
Mukhopadhyay
S
S
Rovera
G
G
D016475:3T3 Cells; D000011:Abelson murine leukemia virus; D000212:Acyclovir / Q000378:metabolism / Q000494:pharmacology*; D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002472:Cell Transformation, Viral / Q000187:drug effects*; D004247:DNA / Q000378:metabolism; D016313:Genes, abl; D066298:In Vitro Techniques; D051379:Mice; D010766:Phosphorylation; D010770:Phosphotransferases / Q000378:metabolism
D004247:DNA; D010770:Phosphotransferases; C021892:nucleoside phosphotransferase; D000212:Acyclovir