African swine fever virus encodes a gene with extensive homology to type II DNA topoisomerases.
Nucleotide sequencing of a virulent African swine fever virus (ASFV) isolate (Malawi LIL20/1) identified an open reading frame of 1191 amino acid residues encoding a protein of 134.9 kDa. This gene mapped to the SalI i and j restriction endonuclease fragments of the ASFV genome. The predicted polypeptide was found to share 21.1% identity over a 1077 amino acid region with the human type II DNA topoisomerase. The sequence is compared to other type II DNA topoisomerases and the possible roles in ASFV replication are discussed.
Baylis
S A
SA
Sir William Dunn School of Pathology, University of Oxford, U.K.
Dixon
L K
LK
Vydelingum
S
S
Smith
G L
GL
D000358:African Swine Fever Virus / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D004250:DNA Topoisomerases, Type II / Q000235:genetics*; D005814:Genes, Viral / Q000235:genetics*; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D014779:Virus Replication
D004250:DNA Topoisomerases, Type II