Augmentation of pulmonary foreign body granulomatous inflammation in mice by lipopolysaccharide: involvement of macrophage activation and tumor necrosis factor-alpha.
We examined the effect of lipopolysaccharide (LPS) on dextran bead-induced lung granulomas using LPS responder (C3H/HeN) and nonresponder (C3H/HeJ) mice. LPS augmented granuloma sizes, TNF-alpha and N-acetyl-beta-D-glucosaminidase levels of lung extracts in C3H/HeN, but not in C3H/HeJ mice. Granuloma macrophages of C3H/HeN mice produced higher levels of superoxide anion and TNF-alpha than those of C3H/HeJ mice. Taken together with our previous report that macrophages and macrophage-derived cytokines are essential for the development of lesions, the present results suggest that activation of macrophages plays an important role in the development and augmentation of pulmonary granulomas.
Kasama
T
T
First Department of Internal Medicine, Showa University School of Medicine, Tokyo, Japan.
Kobayashi
K
K
Yamagata
N
N
Iwabuchi
H
H
Matsuda
T
T
Nakatani
K
K
Kasahara
K
K
Takahashi
T
T
D000118:Acetylglucosaminidase / Q000032:analysis; D000704:Analysis of Variance; D000818:Animals; D006099:Granuloma / Q000139:chemically induced* / Q000378:metabolism / Q000473:pathology; D007150:Immunohistochemistry; D008070:Lipopolysaccharides; D008171:Lung Diseases; D008262:Macrophage Activation; D008264:Macrophages / Q000378:metabolism; D051379:Mice; D008809:Mice, Inbred C3H; D013481:Superoxides / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*
D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; D013481:Superoxides; D000118:Acetylglucosaminidase