Inosine-containing primers in human papillomavirus detection by polymerase chain reaction.
A PCR protocol has been developed using inosine-containing primers for human papilloma virus (HPV) detection. The HPV16 L1 region from CaSki-positive cells was efficiently amplified by a single reaction and directly analyzed by agarose gel electrophoresis. The method was found to be sensitive and reproducible, and it easy to use for HPV typing and screening.
Novelli
G
G
Department of Public Health and Cell Biology, 2nd University of Rome, Italy.
Gennarelli
M
M
De Santis
L
L
Angeloni
P
P
Dallapiccola
B
B
D001483:Base Sequence; D006801:Humans; D066298:In Vitro Techniques; D007288:Inosine / Q000737:chemistry*; D027383:Papillomaviridae / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*
D007288:Inosine