[Genes of the lipase family: comparison of nucleic and proteinic sequences].
Vertebrates' plasmatic apolipoproteins and a few number of lipases in their metabolism present sequence homologies. They are grouped in genes families. The four exons apolipoproteins gene family includes nine human genes: the divergence rate of their sequences allows to place the first ancestral gene very high in the phylogenetic tree of the evolution. However, a more recent duplication of apolipoprotein C-I gene dating from 40 millions years, may be a phylogenetic marker for the radiation of Monkeys. Pancreatic lipase and isoforms, lipoprotein-lipase and hepatic triacylglycerol-lipase form by their homologies a "superfamily" of genes, which also includes yolk proteins of Dipterians eggs. Sequence homologies of PL, LPL and HL are analysed and compared with multiple alignments of amino-acids and nucleotides on spreadsheets. From these comparisons we may characterize four classes of phylogenetic markers: 1) repetitive DNA sequence (Alu, B1, PRE-1) appeared during Mammals evolution, 2) short insertions or deletions (within N-terminal domain) and a gene conversion in guinea-pig lineage, 3) a progressive reduction of intron number during the lipases evolution, 4) several duplications of genes which have produced the five genes of this superfamily currently known in the human genome.
Raisonnier
A
A
Laboratoire des Lipides et des Lipoprotéines, CHU Pitié-Salpêtrère, Paris.
Brault
D
D
Noe
L
L
Etienne
J
J
Hamelin
J
J
Chuat
J C
JC
Dugail
I
I
Quignard-Boulange
A
A
Lavau
M
M
Galibert
F
F
D000595:Amino Acid Sequence; D000818:Animals; D001053:Apolipoproteins / Q000235:genetics / Q000648:ultrastructure; D001483:Base Sequence; D008049:Lipase / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012689:Sequence Homology, Nucleic Acid
D001053:Apolipoproteins; D008049:Lipase
Les gènes de la famille des lipases: comparaison des séquences nucléiques et protéiques.