title: Sequence analysis of the large (L) protein of simian virus 5.
abstract: The complete nucleotide sequence of the large (L) protein gene of simian virus 5 (SV5) was determined from cDNA of the genomic RNA and mRNA, and found to be 6804 bases in length, exclusive of a poly(A) tract. The sequence contained an open reading frame of 6765 nucleotides encoding 2255 amino acids. Results of dot matrix comparisons of the L protein of SV5 with those of human parainfluenza type 3 virus and Sendai virus indicated that there are five conserved domains, and that each domain contains characteristic sequence(s). The L protein of SV5 was detected in purified virions using antiserum directed against an oligopeptide corresponding to the N-terminal region.
authors.affiliation: Department of Microbiology, Mie University School of Medicine, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Higuchi
authors.forename: Y
authors.initials: Y
authors.lastname: Miyahara
authors.forename: M
authors.initials: M
authors.lastname: Kawano
authors.forename: M
authors.initials: M
authors.lastname: Tsurudome
authors.forename: H
authors.initials: H
authors.lastname: Matsumura
authors.forename: S
authors.initials: S
authors.lastname: Kusagawa
authors.forename: H
authors.initials: H
authors.lastname: Komada
authors.forename: M
authors.initials: M
authors.lastname: Nishio
authors.forename: Y
authors.initials: Y
authors.lastname: Ito
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004277:DNA, Single-Stranded / Q000235:genetics; D008969:Molecular Sequence Data; D010222:Parainfluenza Virus 1, Human / Q000235:genetics; D010224:Parainfluenza Virus 3, Human / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics; D015301:Retroviruses, Simian / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D014764:Viral Proteins / Q000032:analysis / Q000235:genetics*; D014771:Virion / Q000737:chemistry
chemical_list: D004277:DNA, Single-Stranded; D012333:RNA, Messenger; D012367:RNA, Viral; D014764:Viral Proteins; C075512:L protein, Simian parainfluenza virus 5