The biological reductive capacity of tissues is decreased following exposure to oxidative stress: a cyclic voltammetry study of irradiated rats.
The reductive capacity of rat tissue homogenates and body fluids was determined by cyclic voltammetric measurements. The reductive capacity of rat lung, liver and kidney homogenates was significantly reduced four days after total body gamma-ray irradiation with 5.5 Gy as compared to controls. In parallel, reduced ability of the irradiated organ homogenates to scavenge hydroxyl radicals and to destroy hydrogen peroxide was recorded. However, no difference in their superoxide dismutase activity was found. The possible use of cyclic voltammetry as a method for qualitative evaluation of the ability of biological tissues to cope with oxidative stress is discussed.
Kohen
R
R
Department of Pharmacy, Hebrew University of Jerusalem.
Tirosh
O
O
Gorodetsky
R
R
D000818:Animals; D001826:Body Fluids / Q000528:radiation effects*; D002384:Catalysis; D017097:Electric Impedance; D016166:Free Radical Scavengers; D005609:Free Radicals; D005720:Gamma Rays; D006878:Hydroxides; D017665:Hydroxyl Radical; D007668:Kidney / Q000528:radiation effects*; D008099:Liver / Q000528:radiation effects*; D008168:Lung / Q000528:radiation effects*; D010084:Oxidation-Reduction; D051381:Rats; D011919:Rats, Inbred Strains; D013482:Superoxide Dismutase / Q000378:metabolism; D014916:Whole-Body Irradiation
D016166:Free Radical Scavengers; D005609:Free Radicals; D006878:Hydroxides; D017665:Hydroxyl Radical; D013482:Superoxide Dismutase