A reexamination of the stopped-flow measured hydrogen-deuterium exchange rates in nucleic acid double helices.
Using the deuteration labelling method in conjunction with an improved stopped-flow instrument, we have reexamined the proton exchange process in poly(dA-dT).poly(dA-dT). A single proton exchange class is found with a rate constant at 20 degrees C of 0.4 S-1. This exchange rate is independent of buffer concentration. From the comparison of these results with those obtained in a former study where two rates were measured, it is concluded that the present deuteration signal corresponds to the exchange of the amino protons, whereas the imino proton exchange is not detected.
Girardet
J L
JL
Laboratoire de Biophysique Moléculaire et Cellulaire, DBMS, (UA 520 CNRS) CENG, Grenoble, France.
Ramstein
J
J
D002021:Buffers; D006152:Cyclic GMP / Q000737:chemistry; D003903:Deuterium / Q000737:chemistry*; D006859:Hydrogen / Q000737:chemistry*; D007700:Kinetics; D011067:Poly dA-dT / Q000737:chemistry*
D002021:Buffers; D011067:Poly dA-dT; D006859:Hydrogen; D003903:Deuterium; D006152:Cyclic GMP