PCR cloning of the cDNA of rabbit skeletal muscle protein phosphatase inhibitor-2.
The coding region of the cDNA of protein phosphatase inhibitor-2 was determined by polymerase chain reaction amplification. The cDNA clone consisted of 621 nucleotides, and encoded 204 amino acids. The deduced amino-acid sequence was identical with that of the sequence reported by chemical sequencing methods.
Zhang
Z
Z
Department of Biochemistry and Molecular Biology, University of Miami School of Medicine, Florida 33101.
Bai
G
G
Lee
E Y
EY
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D009124:Muscle Proteins / Q000235:genetics*; D009132:Muscles / Q000502:physiology*; D009838:Oligodeoxyribonucleotides; D010749:Phosphoprotein Phosphatases / Q000037:antagonists & inhibitors; D016133:Polymerase Chain Reaction / Q000379:methods; D011506:Proteins / Q000235:genetics*; D011817:Rabbits; D000097847:Protein Phosphatase Inhibitory Proteins
D004247:DNA; D009124:Muscle Proteins; D009838:Oligodeoxyribonucleotides; D010749:Phosphoprotein Phosphatases; D011506:Proteins; C024833:protein phosphatase inhibitor-2; D000097847:Protein Phosphatase Inhibitory Proteins