Multimerization of ICP0, a herpes simplex virus immediate-early protein.
ICP0, a herpes simplex virus immediate-early gene product, is a highly phosphorylated nuclear protein that is a potent activator of virus and host genes. Using biochemical and genetic assays employing plasmids encoding mutant forms of ICP0 and a recombinant adenovirus that expresses ICP0, we mutant forms of ICP0 and a recombinant adenovirus that expresses ICP0, we provide evidence that the protein multimerizes. Some mutant forms of ICP0 were transdominant and interfered with activation of a target reporter gene or with complementation of an ICP0-minus virus.
Chen
J
J
Department of Microbiology, Columbia University, New York, New York 10032.
Panagiotidis
C
C
Silverstein
S
S
D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D004252:DNA Mutational Analysis; D005816:Genetic Complementation Test; D017874:Immediate-Early Proteins; D011487:Protein Conformation; D018139:Simplexvirus / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic; D044767:Ubiquitin-Protein Ligases; D014709:Vero Cells; D014762:Viral Interference; D014764:Viral Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*
D017874:Immediate-Early Proteins; D014764:Viral Proteins; D044767:Ubiquitin-Protein Ligases; C051804:Vmw110 protein, Human herpesvirus 1