L-glutamate transporter derived from mRNAs of primary glial cultures: expression in Xenopus laevis oocytes.
A high-affinity sodium-dependent L-glutamate transporter was expressed in Xenopus oocytes after microinjection of poly(A)+ RNA from primary astrocyte cultures from rat brain cortex. mRNA-induced L-glutamate transport was saturable by substrate and shows kinetic features similar to those found in intact glial cell preparations. L-Glutamate accumulation was prevented by rising the external K+ concentration or by coincubation with L-, D-aspartate or D-glutamate. After fractionation by sucrose density gradient, the mRNA encoding for the expressed L-glutamate transporter from glial cells was found in fractions containing messages of 2.05-2.9 kilobases (kb) in length.
Alcántara
R
R
Centro de Biología Molecular, Facultad de Ciencias, Universidad Autónoma de Madrid, Spain.
Casado
M
M
Olivares
L
L
Giménez
C
C
Aragón
C
C
D000469:Alkaline Phosphatase / Q000378:metabolism; D027322:Amino Acid Transport System X-AG; D000818:Animals; D002478:Cells, Cultured; D006023:Glycoproteins / Q000096:biosynthesis / Q000378:metabolism*; D007700:Kinetics; D009457:Neuroglia / Q000378:metabolism*; D009865:Oocytes / Q000378:metabolism*; D011061:Poly A / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D017208:Rats, Wistar; D014982:Xenopus laevis
D027322:Amino Acid Transport System X-AG; D006023:Glycoproteins; D012333:RNA, Messenger; D011061:Poly A; D000469:Alkaline Phosphatase