[Observation on the changes in the frequency of end-plate potentials evoked by sera with different antibodies from patients with myasthenia gravis in nerve-muscle preparation of rats].
Using glass microelectrode recording the effect of sera with presynaptic membrane (PrM) antibody and acetylcholine receptor (AchR) antibody on the frequency of miniature end-plate potentials (MEPPs) was tested in the phrenic nerve-diaphragm preparation of rats. Sera from 5 health control persons did not affect the MEPP frequency. Twenty tested patient sera were identified to include 3 cases with AChR antibody, 6 cases with PrM antibody, 5 cases with both and 6 cases without both antibodies. The results showed that 2 cases with PrM antibody first increased for 1 to 2 minutes and then decreased the MEPP frequency. The MEPP frequency was decreased in total 11 cases, including 2 cases with AChR antibody, 3 cases with PrM antibody, 4 cases with both and 2 cases without both antibodies. The MEPP frequency was not changed in total 7 cases, including 4 cases without both antibodies and 1 case from each other 3 kinds of sera. The results demonstrated that serum from some patients with myasthenia gravis, especially from those with PrM antibody has some factors which act on the motor presynaptic ending.
Lu
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Institute of Neurology, Shanghai Medical University.
D000328:Adult; D000368:Aged; D000818:Animals; D001323:Autoantibodies / Q000502:physiology*; D003964:Diaphragm / Q000502:physiology; D005260:Female; D006801:Humans; D008297:Male; D008564:Membrane Potentials; D008875:Middle Aged; D009045:Motor Endplate / Q000502:physiology*; D009157:Myasthenia Gravis / Q000276:immunology* / Q000503:physiopathology; D010791:Phrenic Nerve / Q000502:physiology; D051381:Rats; D011950:Receptors, Cholinergic / Q000276:immunology*; D017981:Receptors, Neurotransmitter / Q000276:immunology*
D001323:Autoantibodies; D011950:Receptors, Cholinergic; D017981:Receptors, Neurotransmitter