Phosphatidylethanolamine is the donor of the ethanolamine residue linking a glycosylphosphatidylinositol anchor to protein.
Numerous cell surface glycoproteins from eukaryotic organisms including African trypanosomes and budding yeast (Saccharomyces cerevisiae), are anchored to the lipid bilayer by a glycophospholipid, glycosylphosphatidylinositol, covalently linked to the carboxyl terminus of the protein via a phosphoethanolamine bridge. In this paper we describe metabolic labeling experiments aimed at identifying the biosynthetic origin of the ethanolamine residue in the phosphoethanolamine bridge. Using yeast mutants generated by disruption of the ethanolaminephosphotransferase (EPT1) and cholinephosphotransferase (CPT1) genes, we report data consistent with the proposal that the ethanolamine residue is derived from phosphatidylethanolamine.
Menon
A K
AK
Laboratory of Molecular Parasitology, Rockefeller University, New York, New York 10021.
Stevens
V L
VL
D002798:Diacylglycerol Cholinephosphotransferase / Q000235:genetics / Q000378:metabolism*; D019856:Ethanolamine; D004982:Ethanolaminephosphotransferase / Q000235:genetics / Q000378:metabolism*; D004983:Ethanolamines / Q000378:metabolism; D005800:Genes, Fungal; D006017:Glycolipids / Q000378:metabolism*; D017261:Glycosylphosphatidylinositols; D007294:Inositol / Q000378:metabolism; D008051:Lipid Bilayers; D016296:Mutagenesis; D010714:Phosphatidylethanolamines / Q000378:metabolism*; D010716:Phosphatidylinositols / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*
D004983:Ethanolamines; D006017:Glycolipids; D017261:Glycosylphosphatidylinositols; D008051:Lipid Bilayers; D010714:Phosphatidylethanolamines; D010716:Phosphatidylinositols; D007294:Inositol; D019856:Ethanolamine; D004982:Ethanolaminephosphotransferase; D002798:Diacylglycerol Cholinephosphotransferase