title: Detection of single HPV copies in SiHa cells by in situ polymerase chain reaction (in situ PCR) combined with immunoperoxidase and immunogold-silver staining (IGSS) techniques.
abstract: Detection of HPV by means of in situ hybridization techniques may often present problems if the number of HPV copies is too small, especially when using nonradioactive detection systems. A new way of amplifying extremely small amounts of virus DNA copies is the polymerase chain reaction (PCR), which is mostly used in vitro. In this study, we present a method for in situ PCR combined with immunogold-silver staining (IGSS) and immunoperoxidase (IMP) methods which allows the detection of single copies of HPV-DNA in SiHa cells. This method may have wide applications in routine diagnostic histopathology.
authors.affiliation: Department of Pathology, University Hospital, Uppsala, Sweden.
authors.forename: I
authors.initials: I
authors.lastname: Zehbe
authors.forename: G W
authors.initials: GW
authors.lastname: Hacker
authors.forename: E
authors.initials: E
authors.lastname: Rylander
authors.forename: J
authors.initials: J
authors.lastname: Sällström
authors.forename: E
authors.initials: E
authors.lastname: Wilander
mesh_terms: D001483:Base Sequence; D004279:DNA, Viral / Q000032:analysis* / Q000235:genetics; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry / Q000379:methods; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods; D014407:Tumor Cells, Cultured; D002583:Uterine Cervical Neoplasms / Q000382:microbiology*
chemical_list: D004279:DNA, Viral; D009838:Oligodeoxyribonucleotides