The detection of Alcelaphine herpesvirus-1 DNA by in situ hybridization of tissues from rabbits affected with malignant catarrhal fever.
Tissue sections and cultured lymphocytes from rabbits clinically affected following experimental infection with Alcelaphine herpesvirus-1 (AHV-1) were assessed for the presence of viral DNA by in situ hybridization with the cloned major HindII repeat sequence of this virus. Small numbers of virus-infected cells were consistently detected only in submandibular lymph nodes, while other tissues showed no evidence of viral DNA. Virus titration in culture suggested that there were higher titres of virus in the lymph nodes, spleen and lung of infected animals than in the kidney or peripheral blood lymphocytes and confirmed the low level of virus in these animals. Substantially more viral DNA was detected by in situ hybridization in lymphocytes following at least 24 h of culture, suggesting that viral replication is normally repressed by the host.
Bridgen
A
A
Moredun Research Institute, Edinburgh, U.K.
Munro
R
R
Reid
H W
HW
D000818:Animals; D002478:Cells, Cultured; D046508:Culture Techniques; D004279:DNA, Viral / Q000032:analysis*; D006564:Herpesviridae / Q000737:chemistry / Q000302:isolation & purification*; D006566:Herpesviridae Infections / Q000150:complications / Q000382:microbiology*; D008198:Lymph Nodes / Q000382:microbiology*; D008214:Lymphocytes / Q000382:microbiology; D008304:Malignant Catarrh / Q000209:etiology / Q000382:microbiology*; D009693:Nucleic Acid Hybridization; D011817:Rabbits
D004279:DNA, Viral