A triple-resonance pulse scheme for selectively correlating amide 1HN and 15N nuclei with the 1H alpha proton of the preceding residue.
A 3D 1H-15N-13C triple resonance experiment is presented that contains exclusively cross peaks between the 1HN and 15N nuclei of one residue with the H alpha of the preceding residue. The pulse sequence, designed to minimize the time coherence, is transverse on nuclei with short T2 values. The experiment consists of coherence transfers via one-bond couplings from the HN via N, CO, C alpha to the H alpha and back to the HN for detection; it is called HN(COCA)HA. The experiment was tested on uniformly 15N- and 13C-enriched T4 lysozyme.
Clubb
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Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115.
Wagner
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D000577:Amides / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D009587:Nitrogen Isotopes; D011522:Protons
D000577:Amides; D009587:Nitrogen Isotopes; D011522:Protons