In vitro karyotypic and immunophenotypic characterisation of primitive neuroectodermal tumours: similarities to malignant gliomas.
Monoclonal antibody (Mab) mediated immunotherapy of brain tumours requires the identification of tumour-restricted cell surface antigens. We have characterised four primitive neuroectodermal tumours, which included pineoblastoma, medulloblastoma and ependymoblastoma cultures, that demonstrated in vitro evidence of malignant behaviour (anchorage-independent growth and nu/nu xenograft tumour formation). The cytogenetic findings ranged from normal G-banded and Q-banded karyotypes through mixed near-diploid/hyperdiploid. These cultures resembled the cell surface immunophenotypic spectrum of malignant gliomas. They were distinguished from normal glia in vitro by the expression of restricted fetal mesenchymal, neuronal, myoblastic, melanocytic, epidermal, chondrocytic, lymphoid and epithelial antigens. Certain antigens appeared sufficiently represented among central nervous system (CNS) neoplasms to afford potential targets for Mab-mediated immunotherapy.
Jennings
M T
MT
Vanderbilt University Medical Center, Nashville, Tennessee 37232-3375.
Jennings
D L
DL
Ebrahim
S A
SA
Johnson
M D
MD
Turc-Carel
C
C
Philip
T
T
Philip
I
I
Lapras
C
C
Shapiro
J R
JR
D000293:Adolescent; D000911:Antibodies, Monoclonal; D001932:Brain Neoplasms / Q000737:chemistry / Q000235:genetics / Q000473:pathology; D016543:Central Nervous System Neoplasms / Q000737:chemistry / Q000235:genetics / Q000473:pathology*; D002528:Cerebellar Neoplasms / Q000737:chemistry / Q000235:genetics / Q000473:pathology; D004806:Ependymoma / Q000737:chemistry / Q000235:genetics / Q000473:pathology; D005260:Female; D005910:Glioma / Q000737:chemistry / Q000235:genetics / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D016130:Immunophenotyping; D007223:Infant; D007621:Karyotyping; D008297:Male; D008527:Medulloblastoma / Q000737:chemistry / Q000235:genetics / Q000473:pathology; D009373:Neoplasms, Germ Cell and Embryonal / Q000737:chemistry / Q000235:genetics / Q000473:pathology*; D010871:Pinealoma / Q000737:chemistry / Q000235:genetics / Q000473:pathology; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal