title: The different interactions of a gIII mutant of pseudorabies virus with several different cell types.
abstract: Glycoprotein gIII of pseudorabies virus (PrV) is multifunctional. It plays a role in the stable adsorption of the virus to its host cells by interacting with a cellular heparin-like substance. It also affects both release of mature virus from infected cell types and virulence. Thus, although non-essential for growth in vitro, gIII plays a central role in the biology of the virus. The primary attachment of a mutant, PrV2, which has an in-frame internal deletion and expresses a shortened version of gIII, and of wild-type (wt) virus, to MDBK cells has been shown to occur similarly. To ascertain whether different domains of gIII control the expression of the different biological functions of the gIII protein, we have compared several aspects of virus-host cell interactions of PrV2, of a gIII-null virus, and of wt virus. Our results showed that the deletion of the internal segment of the gIII glycoprotein affects adsorption and virus release differently, i.e. that these two functions of gIII appear to be independent of each other. Furthermore, we observed that although the primary adsorption of PrV2 and wt virus to MDBK cells is similar, PrV2 behaved like a gIII-null mutant with respect to virulence. The apparent contradiction between these two findings was resolved when it was found that although PrV2 binds as well as does wt to some cell types, it binds poorly to other cell types. The functional importance of different domains of gIII in virus adsorption thus differs, depending on the cell type with which the virus interacts.
authors.affiliation: Department of Microbiology and Immunology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232.
authors.forename: L
authors.initials: L
authors.lastname: Zsak
authors.forename: N
authors.initials: N
authors.lastname: Sugg
authors.forename: T
authors.initials: T
authors.lastname: Ben-Porat
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002645:Chickens; D015967:Gene Expression Regulation, Viral; D011558:Herpesvirus 1, Suid / Q000235:genetics / Q000472:pathogenicity*; D007928:Lethal Dose 50; D051379:Mice; D008807:Mice, Inbred BALB C; D016296:Mutagenesis; D011107:Polylysine / Q000494:pharmacology; D011557:Pseudorabies / Q000378:metabolism*; D013329:Structure-Activity Relationship; D014759:Viral Envelope Proteins / Q000235:genetics*; D014774:Virulence; D014779:Virus Replication / Q000187:drug effects / Q000235:genetics
chemical_list: D014759:Viral Envelope Proteins; C042966:pseudorabies virus glycoproteins; D011107:Polylysine