Cloning and sequencing of the gene for the cytoplasmic inorganic pyrophosphatase from the thermoacidophilic archaebacterium Thermoplasma acidophilum.
The gene (ppa) from the thermoacidophilic archaebacterium Thermoplasma acidophilum, encoding the cytoplasmic pyrophosphatase, has been cloned. Two degenerate oligonucleotide probes, synthesized according to the N-terminal amino acid sequence of the isolated protein, were used to screen subgenomic libraries. The DNA-derived amino acid sequence of the archaebacterial enzyme allows, for the first time, comparative studies of cytoplasmic pyrophosphatases to be extended to all three urkingdoms. The archaebacterial pyrophosphatase more closely resembles the eubacterial enzymes on the basis of sequence similarity and subunit size. The majority of amino acid residues considered to be essential for hydrolysis of pyrophosphate seem to have been conserved throughout evolution, as inferred from the results of an alignment of sequences from all three urkingdoms.
Richter
O M
OM
Institut für Biochemie, Medizinische Universität zu Lübeck, Federal Republic of Germany.
Schäfer
G
G
D000595:Amino Acid Sequence; D001483:Base Sequence; D005075:Biological Evolution; D003001:Cloning, Molecular; D003062:Codon; D017124:Conserved Sequence; D003593:Cytoplasm / Q000201:enzymology; D004269:DNA, Bacterial / Q000737:chemistry; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D005798:Genes, Bacterial; D043564:Inorganic Pyrophosphatase; D008969:Molecular Sequence Data; D010957:Plasmids; D011755:Pyrophosphatases / Q000737:chemistry / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D013822:Thermoplasma / Q000201:enzymology* / Q000235:genetics
D003062:Codon; D004269:DNA, Bacterial; D011755:Pyrophosphatases; D043564:Inorganic Pyrophosphatase