Cloning of a B2 bradykinin receptor: examination of the bradykinin binding site by site directed mutagenesis.
A cDNA encoding a bradykinin receptor has been isolated. In oocytes expressing the receptor, bradykinin-induced chloride current is blocked by [Thi5,8 dPhe7]BK and is unaffected by des-Arg9-BK suggesting that the cDNA encodes a classical B2 type receptor. The predicted protein sequence is homologous to other G protein-coupled receptors. Preliminary models of the receptor and BK have been built. Data from mutagenesis experiments designed to test the models is reported.
Freedman
R
R
Syntex Research, Palo Alto, CA 94304.
Jarnagin
K
K
D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D001920:Bradykinin / Q000737:chemistry / Q000378:metabolism*; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D005260:Female; D008958:Models, Molecular; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D051381:Rats; D018002:Receptors, Bradykinin; D017981:Receptors, Neurotransmitter / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D014599:Uterus / Q000378:metabolism
D018002:Receptors, Bradykinin; D017981:Receptors, Neurotransmitter; D004247:DNA; D001920:Bradykinin