title: Metabolism of oxygen radicals in peroxisomes and cellular implications.
abstract: Peroxisomes are subcellular respiratory organelles which contain catalase and H2O2-producing flavin oxidases as basic enzymatic constituents. These organelles have an essentially oxidative type of metabolism and have the potential to carry out different important metabolic pathways. In recent years the presence of different types of superoxide dismutase (SOD) have been demonstrated in peroxisomes from several plant species, and more recently the occurrence of SOD has been extended to peroxisomes from human and transformed yeast cells. A copper,zinc-containing SOD from plant peroxisomes has been purified and partially characterized. The production of hydroxyl and superoxide radicals has been studied in peroxisomes. There are two sites of O2- production in peroxisomes: (1) in the matrix, the generating system being xanthine oxidase; and (2) in peroxisomal membranes, dependent on reduced nicotinamide adenine dinucleotide (NADH), and the electron transport components of the peroxisomal membrane are possibly responsible. The generation of oxygen radicals in peroxisomes could have important effects on cellular metabolism. Diverse cellular implications of oxyradical metabolism in peroxisomes are discussed in relation to phenomena such as cell injury, peroxisomal genetic diseases, peroxisome proliferation and oxidative stress, metal and salt stress, catabolism of nucleic acids, senescence, and plant pathogenic processes.
authors.affiliation: Unidad de Bioquímica Vegetal, Estación Experimental del Zaidín, CSIC, Granada, Spain.
authors.forename: L A
authors.initials: LA
authors.lastname: del Río
authors.forename: L M
authors.initials: LM
authors.lastname: Sandalio
authors.forename: J M
authors.initials: JM
authors.lastname: Palma
authors.forename: P
authors.initials: P
authors.lastname: Bueno
authors.forename: F J
authors.initials: FJ
authors.lastname: Corpas
mesh_terms: D000818:Animals; D002477:Cells / Q000378:metabolism*; D005609:Free Radicals / Q000378:metabolism; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D006878:Hydroxides / Q000378:metabolism; D017665:Hydroxyl Radical; D008830:Microbodies / Q000378:metabolism*; D010944:Plants / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism*
chemical_list: D005609:Free Radicals; D006878:Hydroxides; D013481:Superoxides; D017665:Hydroxyl Radical; D006861:Hydrogen Peroxide; D013482:Superoxide Dismutase