Direct evidence for the alterations in protein structure and conformation upon in vitro nonenzymatic glycosylation.
The formation of nonenzymatic glycosylation products appears to be a link between chronic hyperglycaemia and long-term diabetic complications. However, little is known concerning the glycation-induced modifications in the structure and conformation of proteins, which possibly underlie their altered functional characteristics. This study conveys a direct evidence for and compares the glucose-induced modifications in the conformation of three proteins with various half-lives: bovine serum albumin, human haemoglobin and bovine tendon collagen. These proteins incubated in vitro with glucose in various media containing optionally EDTA and Fe2+ ions contained up to 4-10 times as much attached glucose as did their relevant controls, and the extent of glycation was the highest in the samples incubated under air or in the absence of EDTA. The fluorescence and ESR data indicate that the Trp in albumin molecule, given albumin glycation-induced structural modifications, became more exposed to water surrounding solution whereas the Trp residues of haemoglobin remained shielded from water; also collagen fluorescence derived from the supposedly newly formed covalent crosslinks is vastly increased, and particularly when collagen was glycated under air or in the presence of Fe2+ ions. Possible mechanisms underlying the increased mobility of selected protein domains and glycation-mediated alterations in protein conformation are considered and discussed.
Watala
C
C
Department of Biophysics, University of Lodz, Poland.
Gwozdzinski
K
K
Malek
M
M
D000818:Animals; D002417:Cattle; D003094:Collagen / Q000737:chemistry* / Q000378:metabolism; D004492:Edetic Acid / Q000494:pharmacology; D004578:Electron Spin Resonance Spectroscopy; D005296:Ferrous Compounds / Q000494:pharmacology; D005947:Glucose / Q000378:metabolism*; D006023:Glycoproteins / Q000737:chemistry*; D006031:Glycosylation; D006454:Hemoglobins / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D015394:Molecular Structure; D011487:Protein Conformation; D012710:Serum Albumin, Bovine / Q000737:chemistry* / Q000378:metabolism; D013050:Spectrometry, Fluorescence
D005296:Ferrous Compounds; D006023:Glycoproteins; D006454:Hemoglobins; D012710:Serum Albumin, Bovine; D003094:Collagen; D004492:Edetic Acid; D005947:Glucose