title: Muscarinic receptor subtypes, beta-adrenoceptors and cAMP in the tracheal smooth muscle of conventional and double-muscled calves.
abstract: The total muscarinic (M1 + M2 + M3) and beta-adrenergic receptors in the tracheal smooth muscle of conventional and double-muscled calves were identified and characterized with the non-specific antagonists [3H]quinuclidinyl benzilate ([3H]QNB) and [3H]dihydroalprenolol ([3H]DHA) respectively. Although the quantity of beta-adrenoceptors in double-muscled calves was 25% lower (p < 0.05) than in conventional calves (Bmax = 327 +/- 89 fmol/mg protein), adenylate cyclase assays indicated that the basal adenylate cyclase activity and the (-)-isopropylnoradrenaline (ISO)- and sodium fluoride (NaF)-stimulated values were not significantly different between these calves. However, the density of muscarinic receptors in double-muscled calves was 40% higher (p < 0.01) than in conventional calves (Bmax = 2955 +/- 625 fmol/mg protein). Subtypes of muscarinic receptors were studied with [3H]telenzepine (M1-receptors), [3H]AF-DX 384 (M2-receptors) and [3H]4DAMP (M1 and M3-receptors). It was found that in both double-muscled and conventional calves about 40% of the receptors were of the M3-subtype, the remaining 60% being M2-receptors. From these results, it is suggested that inflammation of the respiratory tract in double-muscled calves may be complicated by an imbalance between the cholinergic bronchoconstrictor and the beta-adrenergic bronchodilator components of the autonomic nervous system.
authors.affiliation: Department of Veterinary Physiology, Faculty of Veterinary Medicine, University of Ghent, Belgium.
authors.forename: E
authors.initials: E
authors.lastname: Roets
authors.forename: C
authors.initials: C
authors.lastname: Burvenich
authors.forename: M
authors.initials: M
authors.lastname: Roberts
mesh_terms: D000818:Animals; D002417:Cattle / Q000145:classification*; D000242:Cyclic AMP / Q000378:metabolism*; D009130:Muscle, Smooth / Q000378:metabolism*; D011943:Receptors, Adrenergic, beta / Q000378:metabolism*; D011976:Receptors, Muscarinic / Q000145:classification / Q000378:metabolism*; D014132:Trachea / Q000378:metabolism
chemical_list: D011943:Receptors, Adrenergic, beta; D011976:Receptors, Muscarinic; D000242:Cyclic AMP