Protection from retinal necrosis by passive transfer of monoclonal antibody specific for herpes simplex virus glycoprotein D.
Passive administration of antibody against herpes simplex virus type 1 (HSV-1) has been shown to protect against stromal keratitis and death from encephalitis. Although the exact mechanism by which passively-transferred antibody protects is not known, one of the features of protection by passively-transferred antibody is interference with the ability of the virus to spread within the nervous system. In the experiments reported herein, studies were performed to determine if 8D2, a monoclonal antibody against a type-common epitope of glycoprotein D, could protect mice from retinal necrosis following uniocular anterior chamber inoculation of HSV-1. Mice were protected from retinal necrosis when the antibody was administered 2 hours before virus inoculation or 24 hours after virus inoculation. When antibody was injected 2 hours before virus inoculation, the titer of virus at day 1 p.i. in the injected eyes of antibody-treated and control mice was the same, but by 3 days p.i., the titer of virus in the antibody-treated mice was significantly lower than that recovered from control mice. The titers of virus in the brains and in the uninoculated eyes of antibody-treated mice were also significantly lower than in control mice. The results of these studies suggest that passively-transferred antibody protects against retinal necrosis by limiting spread of virus to the CNS or replication of virus within the CNS.
Atherton
S S
SS
Department of Microbiology and Immunology, University of Miami School of Medicine, FL 33101.
D000818:Animals; D000867:Anterior Chamber / Q000382:microbiology; D000911:Antibodies, Monoclonal / Q000008:administration & dosage*; D000918:Antibody Specificity / Q000276:immunology; D001921:Brain / Q000382:microbiology; D015828:Eye Infections, Viral / Q000517:prevention & control*; D005260:Female; D007116:Immunization, Passive; D051379:Mice; D008807:Mice, Inbred BALB C; D012173:Retinitis / Q000473:pathology / Q000517:prevention & control*; D018139:Simplexvirus / Q000302:isolation & purification; D014759:Viral Envelope Proteins / Q000008:administration & dosage*
D000911:Antibodies, Monoclonal; D014759:Viral Envelope Proteins; C039447:glycoprotein D, Human herpesvirus 1