[Study of toxication of toxoflavin from Pseudomonas cocovenenans to immunocyte and detoxication].
In order to study the toxication of toxoflavin produced by P. cocovenenans to immunocytes and detoxication, the effects of toxoflavin on rabbit blood culture in the presence of PHA and detoxication function of seven drugs towards this system have been observed by 3H-TdR incorporation assay. The more toxoflavin used, the more reduction of cpm value. The results indicated that toxoflavin in 0.5 microgram/ml or more suppressed the growth of cells to even still lower than that of control in which PHA was absent; and when 0.1 microgram/ml of toxoflavin were used, the cpm value began to rise again. The effects of toxoflavin on lymphocyte and other blood cells were studied under the microscope and the naked-eye. The detoxication effects of the seven drugs on toxoflavin were observed and shown that SOD, GSH, Cyt. C and VC were most effective in protecting the lymphocytes from poisoning.
Yue
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Weifang Medical College.
D000818:Animals; D000931:Antidotes / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D003574:Cytochrome c Group / Q000494:pharmacology; D005978:Glutathione / Q000494:pharmacology; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects*; D011549:Pseudomonas / Q000378:metabolism; D011744:Pyrimidinones / Q000302:isolation & purification / Q000633:toxicity; D011817:Rabbits; D013482:Superoxide Dismutase / Q000494:pharmacology; D014227:Triazines / Q000302:isolation & purification / Q000633:toxicity
D000931:Antidotes; D003574:Cytochrome c Group; D011744:Pyrimidinones; D014227:Triazines; C009570:toxoflavin; D013482:Superoxide Dismutase; D005978:Glutathione