Increase in superoxide production by heat-shocked cells of Neurospora crassa, demonstrated by a fluorometric assay.
1. Increase in superoxide production by heat-shocked cells of Neurospora crassa was demonstrated by a fluorometric assay. 2. A sensitive fluorometric assay for the estimation of superoxide anion radical--based on the liberation of 4-methylumbelliferone from 4-methyl-beta-D-umbelliferyl glucopyranoside--is described. 3. Using this system the level of superoxide in the medium of heat-shocked Neurospora crassa cells was found to be consistently higher, in comparison with that of non-shocked cells, cultured at the normal growth temperature of 28 degrees C. 4. Addition of superoxide dismutase to the culture media suppressed the production of 4-methylumbelliferone.
Lin
W S
WS
Department of Biological Sciences, University of Calgary, Alberta, Canada.
Kapoor
M
M
D005470:Fluorometry; D005609:Free Radicals; D005720:Gamma Rays; D006358:Hot Temperature; D006923:Hymecromone / Q000032:analysis; D015394:Molecular Structure; D009492:Neurospora crassa / Q000254:growth & development / Q000378:metabolism*; D015203:Reproducibility of Results; D013482:Superoxide Dismutase / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism*; D013941:Thymine / Q000378:metabolism
D005609:Free Radicals; D013481:Superoxides; D006923:Hymecromone; D013482:Superoxide Dismutase; D013941:Thymine