Foot-and-mouth disease: detection of antibodies in cattle sera by blocking ELISA.
A blocking ELISA was developed for the detection of antibodies to foot-and-mouth disease virus SAT1, SAT2 and SAT3 and for the quantification of antibodies on a single dilution of serum. The avidin-biotin system was used. The test was compared with the liquid-phase ELISA executed at the World Reference Laboratory for foot-and-mouth disease. It was found to have favourable logistics and combined high specificity with high sensitivity. The quantitative test using a single dilution of serum was resource saving and proved to be a reliable and precise method for the assessment of antibody levels.
Sorensen
K J
KJ
Veterinary Research Laboratory, Harare, Zimbabwe.
Madekurozwa
R L
RL
Dawe
P
P
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D005537:Aphthovirus / Q000276:immunology*; D001667:Binding, Competitive; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005536:Foot-and-Mouth Disease / Q000276:immunology*; D012044:Regression Analysis; D012680:Sensitivity and Specificity
D000914:Antibodies, Viral