[Nonenzymatic reduction of Met-Hb under the action of NAD radicals generated in the presence of HAD.H and Fe(II) salts. Antioxidant properties of Met-Hb].
It has been found that superoxide radicals formed at autooxidation of Fe(II) ions or of their complexes with EDTA in the presence of NAD.H cause reduction of met-Hb into deoxy or oxy-form. Under the effect of hydroxyl radicals generated in the ultrasonic field in the presence of NADH reduction of met-Hb into the ferro-form (carboxy-Hb) was observed in Co atmosphere and not in O2 atmosphere. It was induced by a high oxidation rate of oxy-Hb by hydroxyl radicals into met-Hb as compared to carboxy-Hb. Reduction of met-Hb was shown to be accompanied with the formation of NAD+. The role of NAD. radicals in the reduction of met-Hb which acts as an antioxidant in respect to organic free radicals was discussed.
Stepuro
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II
Konovalova
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NV
D000975:Antioxidants / Q000737:chemistry*; D004492:Edetic Acid / Q000737:chemistry; D004798:Enzymes / Q000378:metabolism; D005609:Free Radicals; D006454:Hemoglobins / Q000737:chemistry; D006801:Humans; D007501:Iron / Q000737:chemistry*; D007700:Kinetics; D008706:Methemoglobin / Q000737:chemistry*; D009243:NAD / Q000737:chemistry*; D010084:Oxidation-Reduction; D013481:Superoxides / Q000737:chemistry
D000975:Antioxidants; D004798:Enzymes; D005609:Free Radicals; D006454:Hemoglobins; D009243:NAD; D013481:Superoxides; C100003:deoxyhemoglobin; D008706:Methemoglobin; D004492:Edetic Acid; D007501:Iron
Neénzimaticheskoe vosstanovlenie Met-Hb pod deĭstviem radikalov NAD, generiruemykh v prisutstvii NAD.H i soleĭ Fe(II). Antioksidantnye svoĭstva Met-Hb.