title: Effect of hydroxyl radical on Na(+)-K(+)-ATPase activity of the brain microsomal membranes.
abstract: Sphingomyelin liposomes and brain microsomes were oxidized by exposure to hydrogen peroxide and ferrous ion. Lipid peroxidation were measured by the formation of thiobarbituric acid- reactive substances (TBAR). Hydroxyl radical was detected using the spin- trapping technique. Incubation of sphingomyelin liposomes with H2O2-Fe2+ resulted in an increase in the formation of TBAR. Na(+)-K(+)-ATPase activity was markedly inhibited and the SH group content decreased during incubation of microsomes in the presence of H2O2-Fe2+. Sodium ferulate effectively inhibited TBAR formation, protected Na(+)-K(+)-ATPase activity and prevented the oxidative modification of SH groups. Spin-trapping experiments showed that sodium ferulate effectively scavenged the hydroxyl radicals.
authors.affiliation: Institute of Biophysics, Academia Sinica, Beijing, China.
authors.forename: J W
authors.initials: JW
authors.lastname: Chen
authors.forename: L
authors.initials: L
authors.lastname: Zhang
authors.forename: X
authors.initials: X
authors.lastname: Lian
authors.forename: F
authors.initials: F
authors.lastname: Hwang
mesh_terms: D000818:Animals; D001923:Brain Chemistry; D003373:Coumaric Acids / Q000494:pharmacology*; D004578:Electron Spin Resonance Spectroscopy; D016166:Free Radical Scavengers; D005609:Free Radicals / Q000494:pharmacology; D006861:Hydrogen Peroxide; D006878:Hydroxides / Q000494:pharmacology*; D017665:Hydroxyl Radical; D007425:Intracellular Membranes / Q000187:drug effects / Q000378:metabolism*; D015227:Lipid Peroxidation; D008081:Liposomes; D008861:Microsomes / Q000378:metabolism; D011817:Rabbits; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*; D013438:Sulfhydryl Compounds / Q000032:analysis
chemical_list: D003373:Coumaric Acids; D016166:Free Radical Scavengers; D005609:Free Radicals; D006878:Hydroxides; D008081:Liposomes; D013438:Sulfhydryl Compounds; D017665:Hydroxyl Radical; C004999:ferulic acid; D006861:Hydrogen Peroxide; D000254:Sodium-Potassium-Exchanging ATPase