Differential in vitro inhibition of feline enteric coronavirus and feline infectious peritonitis virus by actinomycin D.
The growth of feline enteric coronavirus strain 79-1683 in whole feline embryo cells was inhibited by the presence of 1 microgram/ml of actinomycin D in the culture fluid. No virus-specific mRNAs could be detected in such cultures and yields of infectious virus were depressed by > 99%. By contrast, the antigenically related feline infectious peritonitis virus strain 79-1146 was unaffected by the presence of actinomycin D, indicating a fundamental difference between the two feline coronavirus strains in their requirements for host-encoded function(s).
Lewis
E L
EL
Department of Veterinary Medicine, Bristol, U.K.
Harbour
D A
DA
Beringer
J E
JE
Grinsted
J
J
D000818:Animals; D002415:Cats / Q000382:microbiology; D003332:Coronaviridae / Q000254:growth & development*; D016765:Coronavirus, Feline / Q000254:growth & development*; D003609:Dactinomycin / Q000494:pharmacology*; D004334:Drug Administration Schedule; D012367:RNA, Viral / Q000378:metabolism; D014779:Virus Replication / Q000187:drug effects*
D012367:RNA, Viral; D003609:Dactinomycin