Direct injection/h.p.l.c. methods for the analysis of drugs in biological samples.
1. Direct injection h.p.l.c. methods for zaprinast, and pantoprazole and its sulphone metabolite were developed. 2. Optimal recovery of pantoprazole and its sulphone metabolite was effected by the absence of transfer losses and the effective adjustment of sample pH on-line. 3. Acetonitrile reduced the recovery of pantoprazole and its sulphone metabolite at acetonitrile concentrations greater than 5% in serum. 4. Direct injection h.p.l.c. methods minimize sample handling losses, reduce human contact with biological samples and are sufficiently accurate and reproducible to be used to support pharmacodynamic and toxicokinetic studies.
Doyle
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E
Department of Metabolism and Pharmacokinetics, SmithKline Beecham, Welwyn, Hertfordshire, UK.
Huber
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R
Picot
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VS
D053799:2-Pyridinylmethylsulfinylbenzimidazoles; D000818:Animals; D001562:Benzimidazoles / Q000032:analysis / Q000097:blood / Q000378:metabolism / Q000652:urine; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006863:Hydrogen-Ion Concentration; D009853:Omeprazole / Q000031:analogs & derivatives; D000077402:Pantoprazole; D004364:Pharmaceutical Preparations / Q000032:analysis*; D054328:Proton Pump Inhibitors; D011688:Purinones / Q000032:analysis / Q000097:blood / Q000652:urine; D051381:Rats; D013450:Sulfones / Q000032:analysis / Q000097:blood / Q000652:urine; D013454:Sulfoxides / Q000032:analysis / Q000097:blood / Q000652:urine; D013997:Time Factors
D053799:2-Pyridinylmethylsulfinylbenzimidazoles; D001562:Benzimidazoles; D004364:Pharmaceutical Preparations; D054328:Proton Pump Inhibitors; D011688:Purinones; D013450:Sulfones; D013454:Sulfoxides; C060592:SK&F 95601; D000077402:Pantoprazole; C011145:zaprinast; D009853:Omeprazole