The rapid serological diagnosis of infectious mononucleosis.
A total of 121 samples of serum collected from 101 patients was tested to determine the sensitivity and specificity of a commercial latex agglutination test for detecting infectious mononucleosis heterophile antibody, a commercial immunofluorescence test for detecting antibody to Epstein-Barr virus capsid antigen and a rapid enzyme immunoassay for detecting antibody to Epstein-Barr virus nuclear antigen. Although the Epstein-Barr virus capsid antigen IgM indirect immunofluorescence test proved to be the most sensitive, false-positive reactions were seen when samples collected from patients with cytomegalovirus, hepatitis A virus, parvovirus and leptospira infection were tested. False-positive reactions were also seen with samples containing rheumatoid factor.
Gray
J J
JJ
Clinical Microbiology and Public Health Laboratory, Addenbrooke's Hospital, Cambridge, U.K.
Caldwell
J
J
Sillis
M
M
D000293:Adolescent; D000328:Adult; D000368:Aged; D000910:Antibodies, Heterophile / Q000097:blood* / Q000276:immunology; D000914:Antibodies, Viral / Q000097:blood*; D000956:Antigens, Viral / Q000097:blood / Q000276:immunology / Q000302:isolation & purification; D036022:Capsid Proteins; D002675:Child, Preschool; D005189:False Positive Reactions; D005455:Fluorescent Antibody Technique; D004854:Herpesvirus 4, Human / Q000276:immunology*; D006801:Humans; D007244:Infectious Mononucleosis / Q000175:diagnosis* / Q000276:immunology; D007841:Latex Fixation Tests; D008875:Middle Aged; D012680:Sensitivity and Specificity
D000910:Antibodies, Heterophile; D000914:Antibodies, Viral; D000956:Antigens, Viral; D036022:Capsid Proteins; C041788:Epstein-Barr viral capsid antigen