Targeting deletion (homoeologous chromosome pairing locus) or addition line single copy sequences from cereal genomes.
We describe here a protocol for obtaining clones containing sequences present in low copy-number from genomic DNA where moderately and highly repeated sequences predominate. Specific chromosomal regions can be targeted by using deletion or addition line material. We have used this protocol to identify a sequence which has been deleted in both the tetraploid and hexaploid wheat mutants for the homoeologous chromosome pairing locus.
Clarke
B
B
Cambridge Laboratory, John Innes Centre for Plant Science Research, Norwich, Norfolk, UK.
Stancombe
P
P
Money
T
T
Foote
T
T
Moore
G
G
D002872:Chromosome Deletion; D003001:Cloning, Molecular; D004247:DNA / Q000302:isolation & purification*; D017886:Durapatite; D016678:Genome; D006882:Hydroxyapatites / Q000494:pharmacology; D009154:Mutation; D011123:Polyploidy; D014908:Triticum / Q000187:drug effects / Q000235:genetics*
D006882:Hydroxyapatites; D004247:DNA; D017886:Durapatite