Abnormal proteins in the cerebrospinal fluid of a patient with Creutzfeldt-Jakob disease following administration of human pituitary growth hormone.
A Brazilian case of Creutzfeldt-Jakob disease in a hypopituitary patient who had received cadaver-derived human pituitary growth hormone between 1968 and 1977 is reported. The clinical diagnosis was confirmed during his lifetime by the demonstration of two abnormal 30-kDa proteins in the cerebrospinal fluid by two-dimensional gel electrophoresis. These proteins, characteristic of Creutzfeldt-Jakob disease, present isoelectric points of 5.1 and 5.2. Furthermore, both proteins migrate as doublets, each one displaying a molecular weight variant of about 29-kDa. This is one of 16 cases of the disease associated to therapy with cadaver-derived human growth hormone and one of the few examples among such cases of confirmation of the clinical diagnosis by biochemical characterization of abnormal proteins in the cerebrospinal fluid.
Macário
M E
ME
Serviço de Endocrinologia, Hospital Universitário Clementino Fraga Filho, Rio de Janeiro, Brasil.
Moura-Neto
V
V
Vaisman
M
M
Araujo
H M
HM
Buescu
A
A
Cordeiro
J G
JG
Chagas
C
C
D000328:Adult; D001938:Brazil; D002556:Cerebrospinal Fluid Proteins / Q000134:cerebrospinal fluid / Q000187:drug effects*; D002908:Chronic Disease; D007562:Creutzfeldt-Jakob Syndrome / Q000134:cerebrospinal fluid* / Q000175:diagnosis / Q000188:drug therapy* / Q000209:etiology; D015180:Electrophoresis, Gel, Two-Dimensional; D013006:Growth Hormone / Q000009:adverse effects / Q000627:therapeutic use*; D006801:Humans; D007018:Hypopituitarism / Q000134:cerebrospinal fluid / Q000150:complications / Q000188:drug therapy; D008297:Male; D008970:Molecular Weight
D002556:Cerebrospinal Fluid Proteins; D013006:Growth Hormone