Acetylene, a mammalian metabolite of 1,1,1-trichloroethane.
1,1,1-Trichloroethane (TCE) is a widely used industrial solvent of low acute toxicity. It is slowly oxidized to trichloroethanol and trichloroacetic acid by cytochrome P-450-dependent mono-oxygenases. Increased inhalative uptake by rats under hypoxia and spin-trapping experiments indicate that TCE is also reductively metabolized to a radical intermediate. Acetylene is formed as a metabolite, suggesting transfer of an additional electron to form the corresponding carbene. Hypoxia and induction of mixed-function mono-oxygenases accelerate the formation of acetylene. Experiments performed in vitro with rat liver microsomal fractions yield analogous results.
Dürk
H
H
Department of Internal Medicine, University of Tübingen, Germany.
Poyer
J L
JL
Klessen
C
C
Frank
H
H
D000114:Acetylene / Q000378:metabolism*; D000818:Animals; D004578:Electron Spin Resonance Spectroscopy; D004980:Ethane / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry; D000860:Hypoxia / Q000378:metabolism; D066298:In Vitro Techniques; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism; D006899:Mixed Function Oxygenases / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D012997:Solvents; D014240:Trichloroethanes / Q000378:metabolism*
D012997:Solvents; D014240:Trichloroethanes; C024566:1,1,1-trichloroethane; D006899:Mixed Function Oxygenases; D004980:Ethane; D000114:Acetylene