title: Spontaneous injury in isolated sheep lungs: role of resident polymorphonuclear leukocytes.
abstract: Perfusion of isolated sheep lungs with homologous blood caused pulmonary hypertension and edema that was not altered by depletion of perfusate polymorphonuclear (PMN) leukocytes (D. B. Pearse et al., J. Appl. Physiol. 66: 1287-1296, 1989). The purpose of this study was to evaluate the role of resident PMN leukocytes in this injury. First, we quantified the content and activation of lung PMN leukocytes before and during perfusion of eight isolated sheep lungs with a constant flow (100 ml.kg-1.min-1) of homologous blood. From measurements of myeloperoxidase (MPO) activity, we estimated that the lungs contained 1.2 x 10(10) PMN leukocytes, which explained why the lung PMN leukocyte content, measured by MPO activity and histological techniques, did not increase significantly with perfusion, despite complete sequestration of 2.0 x 10(9) PMN leukocytes from the perfusate. MPO activities in perfusate and lymph supernatants did not increase during perfusion, suggesting that lung PMN leukocytes were not activated. Second, we perfused lungs from 6 mechlorethamine-treated and 6 hydroxyurea-treated sheep with homologous leukopenic blood and compared them with 11 normal lungs perfused similarly. Despite marked reductions in lung PMN leukocyte concentration, there were no differences in pulmonary arterial pressure, lymph flow, or reservoir weight between groups. Extravascular lung water was greater in both groups of leukopenic lungs. These results suggest that resident PMN leukocytes did not contribute to lung injury in this model.
authors.affiliation: Division of Pulmonary and Critical Care Medicine, Johns Hopkins Medical Institutions, Francis Scott Key Medical Center, Baltimore, Maryland 21224.
authors.forename: D B
authors.initials: DB
authors.lastname: Pearse
authors.forename: J T
authors.initials: JT
authors.lastname: Sylvester
mesh_terms: D000818:Animals; D066298:In Vitro Techniques; D008168:Lung / Q000473:pathology; D055370:Lung Injury; D009504:Neutrophils / Q000473:pathology* / Q000502:physiology; D010477:Perfusion; D009195:Peroxidase / Q000097:blood / Q000378:metabolism; D011652:Pulmonary Circulation; D012756:Sheep
chemical_list: D009195:Peroxidase