Phenotypic properties of liver tumors induced by dehydroepiandrosterone in F-344 rats.
Dehydroepiandrosterone (DHEA), a C19 adrenal steroid hormone, induces peroxisome proliferation in liver cells and is hepatocarcinogenic in the rat. The present study deals with the phenotypic properties of DHEA-induced liver lesions. A majority of the altered areas (80-87%), neoplastic nodules (> 94%) and hepatocellular carcinomas (HCC, 80-100%) lacked the marker enzymes gamma-glutamyltranspeptidase and placental form of glutathione S-transferase (GSTP). Northern blot analysis of HCC from 4 rats revealed no detectable GSTP mRNA. These HCC, however, showed a marked decrease in the staining of glucose-6-phosphatase and adenosine triphosphatase. These results indicate that the phenotypic properties of liver tumors induced by DHEA and amphipathic carboxylate peroxisome proliferators are similar.
Rao
M S
MS
Department of Pathology, Northwestern University Medical School, Chicago, IL 60611.
Subbarao
V
V
Kumar
S
S
Yeldandi
A V
AV
Reddy
J K
JK
D000818:Animals; D015152:Blotting, Northern; D002273:Carcinogens; D006528:Carcinoma, Hepatocellular / Q000139:chemically induced / Q000378:metabolism / Q000503:physiopathology; D003687:Dehydroepiandrosterone; D006651:Histocytochemistry; D008114:Liver Neoplasms, Experimental / Q000139:chemically induced* / Q000235:genetics* / Q000378:metabolism; D008297:Male; D008830:Microbodies / Q000187:drug effects; D010641:Phenotype; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D011916:Rats, Inbred F344
D002273:Carcinogens; D012333:RNA, Messenger; D003687:Dehydroepiandrosterone