Demonstration of ouabain-sensitive, potassium-dependent p-nitrophenylphosphatase activity in the rat cardiac muscle by cobalt-based cytochemistry.
We have utilized cobalt-reaction technique for histochemical and cytochemical demonstration of ouabain-sensitive, K(+)-dependent p-NPPase (Na(+)-K(+)-ATPase). The incubation medium consisted of: cobalt chloride, tricine buffer, p-nitrophenylphosphate, KCl, and phenylalanine. Final pH 7.4. Ultracytochemically, reaction products were localized along the internal side of sarcolemma and its vesicles, T-tubule membrane, and capillary endothelial cells. These results suggest that the method is reliable and can be used to investigate the localization of Na(+)-K(+)-ATPase activity.
Zinchuk
V S
VS
Department of Pathological Anatomy, Kiev Medical Institute, USSR.
Tushevsky
V F
VF
Bulavka
A V
AV
D009597:4-Nitrophenylphosphatase / Q000378:metabolism*; D000818:Animals; D003035:Cobalt; D004730:Endothelium, Vascular / Q000201:enzymology; D006651:Histocytochemistry; D008297:Male; D009206:Myocardium / Q000201:enzymology*; D010042:Ouabain / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D012508:Sarcolemma / Q000201:enzymology; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism
D003035:Cobalt; D010042:Ouabain; D009597:4-Nitrophenylphosphatase; D000254:Sodium-Potassium-Exchanging ATPase; C018021:cobaltous chloride