Expression of histone H3 cell cycle-related gene, vimentin and MYC genes in pediatric brain tumors. A preliminary analysis showing the different malignant cell growth potential.
Eleven pediatric brain tumors were studied for the histone H3, Vimentin and MYC gene expression. H3, an S phase cell cycle-related gene (ccr), was found prevalently expressed in tumors with a high mitotic index (MI). Vimentin gene, which contributes to maintaining the cell structure but is also demonstrated to be an early responder gene to growth stimulation was found variously expressed. The different expression of Vimentin gene in the examined samples suggests the active proliferation of the tumor cells. Analysis of MYC gene expression was found increased only in a mesenchymal chondrosarcoma while in other samples MYC mRNA was undetectable. Medulloblastoma, chondrosarcoma, and choroid plexus carcinoma have high S phase H3 gene expression associated with a high MI. Differently an astrocytoma shows a low MI associated with high H3 gene expression. This first preliminary report of H3, Vimentin and MYC gene expression in brain tumors demonstrates that malignant cells are characterized by a different gene expression and different growth potentials.
Stenger
A M
AM
Department of Pediatric N. II, Semmelweis University Medical School, Budapest, Hungary.
Garré
M L
ML
Andreussi
L
L
Cama
A
A
Callea
F
F
Brisigotti
M
M
Fabbretti
G
G
Di Martino
D
D
Tonini
G P
GP
D000293:Adolescent; D000328:Adult; D001932:Brain Neoplasms / Q000235:genetics* / Q000473:pathology / Q000601:surgery; D002453:Cell Cycle; D002455:Cell Division; D002675:Child, Preschool; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D016259:Genes, myc; D006657:Histones / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D007223:Infant; D008297:Male; D008940:Mitotic Index; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000235:genetics; D009380:Neoplasms, Nerve Tissue / Q000235:genetics* / Q000473:pathology / Q000601:surgery; D016271:Proto-Oncogene Proteins c-myc / Q000096:biosynthesis* / Q000235:genetics; D016896:Treatment Outcome; D014746:Vimentin / Q000096:biosynthesis* / Q000235:genetics
D006657:Histones; D009363:Neoplasm Proteins; D016271:Proto-Oncogene Proteins c-myc; D014746:Vimentin