title: Development of inositol 1,4,5-trisphosphate 3-kinase immunoreactivity in cerebellar Purkinje cells in vivo and in vitro.
abstract: Development profiles in vivo and in vitro of inositol 1,4,5-trisphosphate 3-kinase (IP3K) were investigated immunohistochemically in the cerebellar Purkinje cells. In in vivo preparations of rat cerebellum, IP3K immunoreactivity appeared in Purkinje cell bodies and dendrites shortly after birth, increased rapidly by postnatal day 5, and was subsequently confined to their dendritic processes by day 20. The appearance and shift of IP3K immunoreactivity in Purkinje cells showed an identical time course even when Purkinje cells were placed under culture conditions commencing on day 0, suggesting that Purkinje cells have their own biological clock on the expression of IP3K in the absence of external influences.
authors.affiliation: Department of Medicine, University of British Columbia, Vancouver, Canada.
authors.forename: M
authors.initials: M
authors.lastname: Mizuguchi
authors.forename: M
authors.initials: M
authors.lastname: Yamada
authors.forename: S G
authors.initials: SG
authors.lastname: Rhee
authors.forename: S U
authors.initials: SU
authors.lastname: Kim
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D002531:Cerebellum / Q000201:enzymology*; D003712:Dendrites / Q000201:enzymology; D007150:Immunohistochemistry; D009434:Neural Pathways / Q000201:enzymology; D010770:Phosphotransferases / Q000276:immunology / Q000378:metabolism*; D017853:Phosphotransferases (Alcohol Group Acceptor); D011689:Purkinje Cells / Q000201:enzymology*; D051381:Rats; D015290:Second Messenger Systems
chemical_list: D000911:Antibodies, Monoclonal; D010770:Phosphotransferases; D017853:Phosphotransferases (Alcohol Group Acceptor); C051038:Inositol 1,4,5-trisphosphate 3-kinase