title: Preparative isolation of angiotensin-converting enzyme from human lung.
abstract: Angiotensin-converting enzyme from human lung was purified to apparent homogeneity using a five-step purification procedure consisting of ammonium sulfate precipitation, ion-exchange chromatography on DEAE Sephadex A-50, gel permeation on Sephadex G-200, chromatofocusing on a polybuffer exchange (PBE 94) column and high-performance liquid chromatographic gel permeation on a Bio-Sil TSK-250 column. This procedure gave an approximately 700-fold purification with a 20% yield compared to a 550-fold purification and a 1% yield with an affinity chromatography-based procedure. The 20-fold greater yield of the five-step procedure offers a major advantage for preparative use in the structural characterization of angiotensin-converting enzyme.
authors.affiliation: Department of Cell Biology and Medicine, University of Alabama, Birmingham 35294.
authors.forename: Q C
authors.initials: QC
authors.lastname: Meng
authors.forename: S J
authors.initials: SJ
authors.lastname: King
authors.forename: K E
authors.initials: KE
authors.lastname: Branham
authors.forename: L J
authors.initials: LJ
authors.lastname: Delucas
authors.forename: B
authors.initials: B
authors.lastname: Lorber
authors.forename: S
authors.initials: S
authors.lastname: Oparil
mesh_terms: D015153:Blotting, Western; D002846:Chromatography, Affinity / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002852:Chromatography, Ion Exchange / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008168:Lung / Q000201:enzymology*; D007703:Peptidyl-Dipeptidase A / Q000302:isolation & purification*
chemical_list: D007703:Peptidyl-Dipeptidase A