Characterization of the tachykinin NK2 receptor subtype in the rabbit pulmonary artery.
Contractile responses to neurokinin A (NKA), neuropeptide gamma(NP gamma), and the NK2 receptor-selective analogs [Lys5,MeLeu9,Nle10]NKA(4-10) and MDL 28,564 were determined in the endothelium-denuded rabbit pulmonary artery. Responses to NKA, NP gamma, and [Lys5,MeLeu9,Nle10]NKA(4-10) were antagonized by the NK2 receptor antagonist MDL 29,913, with pA2 values of 6.67, 6.46, and 7.32, respectively. Autoradiographic studies failed to demonstrate any specific binding sites for [125I]-iodohistidyl NKA (INKA) over the pulmonary artery. These data suggest the presence in rabbit pulmonary artery of an unusual "nonclassical" NK2 receptor subtype, which appears to lack affinity for INKA.
Xiao
X H
XH
School of Physiology and Pharmacology, University of New South Wales, Sydney, Australia.
Mussap
C J
CJ
Burcher
E
E
D000595:Amino Acid Sequence; D000818:Animals; D001345:Autoradiography; D005260:Female; D008297:Male; D008969:Molecular Sequence Data; D009119:Muscle Contraction / Q000187:drug effects*; D009131:Muscle, Smooth, Vascular / Q000187:drug effects*; D010456:Peptides, Cyclic / Q000494:pharmacology; D011651:Pulmonary Artery / Q000737:chemistry*; D011817:Rabbits; D017981:Receptors, Neurotransmitter / Q000037:antagonists & inhibitors / Q000737:chemistry* / Q000145:classification; D018039:Receptors, Tachykinin; D015320:Tachykinins / Q000037:antagonists & inhibitors* / Q000494:pharmacology
D010456:Peptides, Cyclic; D017981:Receptors, Neurotransmitter; D018039:Receptors, Tachykinin; D015320:Tachykinins; C073382:MDL 29913