[Effects of dauricine on action potentials and slow inward currents of guinea pig ventricular papillary muscles].
Effects of dauricine (Dau) on the action potentials (AP), the slow action potentials (SAP), and the slow inward currents (Isi) of guinea pig ventricular papillary muscles were observed by means of intracellular microelectrode and single sucrose gap voltage clamp technique. In the early stage, Dau shortened action potential duration 100 (APD100) and effective refractory period (ERP) (ERP/APD < 1; P < 0.01), but did not affect APD20 and other parameters. In the late stage, Dau prolonged APD100, ERP, and APD20, significantly decreased action potential amplitude (APA), maximum velocity (Vmax), and overshot (OS) (ERP/APD > 1; P < 0.01), greatly diminished APA and OS of SAP induced by isoprenaline (P < 0.01), and remarkably inhibited Isi (P < 0.01). The results suggested that Dau exerted an inhibitory effect on Na+, Ca2+, and K+ channels.
Li
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SN
Department of Pharmacology, China Medical University, Shenyang.
Zhang
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KY
D000200:Action Potentials / Q000187:drug effects; D000470:Alkaloids; D000818:Animals; D000889:Anti-Arrhythmia Agents / Q000494:pharmacology*; D044182:Benzylisoquinolines; D000252:Calcium-Transporting ATPases / Q000187:drug effects; D005260:Female; D006168:Guinea Pigs; D066298:In Vitro Techniques; D007546:Isoquinolines / Q000494:pharmacology*; D008297:Male; D010210:Papillary Muscles / Q000502:physiology*; D044005:Tetrahydroisoquinolines
D000470:Alkaloids; D000889:Anti-Arrhythmia Agents; D044182:Benzylisoquinolines; D007546:Isoquinolines; D044005:Tetrahydroisoquinolines; C035934:dauricine; D000252:Calcium-Transporting ATPases