title: Antagonism by potassium of the inhibition of (Na(+)+K+) ATPase produced by an endogenous digitalis-like compound extracted from mammalian heart.
abstract: The antagonism by potassium of (Na(+)+K+)ATPase inhibition was analyzed in order to further study the presence of endogenous digitalis-like activity in mammalian heart. Digoxin and a partially purified extract prepared from guinea pig cardiac tissue inhibited (Na(+)+K+)ATPase, also obtained from guinea pig heart, in a dose-dependent manner in agreement with Michaelis-Menten kinetics. The effects of both inhibitors were antagonized by increasing the KCl concentration from 0.5 to 5 mM. Hunter-Downs plots were linear, suggesting that the interaction between (Na(+)+K+)ATPase and either digoxin or the extract could be considered as competitive. Ks estimated for KCl from these plots was similar to the K0.5 value obtained by activating the enzyme with KCl, and thus suggesting that both inhibitors, digoxin and the extract, were acting on (Na(+)+K+)ATPase. Digoxin and the extract were able to inhibit [3H]ouabain specific binding to the enzyme in a dose-dependent manner in agreement with a competitive interaction. Ki values estimated from binding experiments were similar to those calculated from Hunter-Downs plots. Data strongly suggest the presence in guinea pig heart of an endogenous compound which is able to inhibit (Na(+)+K+)ATPase solely by binding to the digitalis receptors.
authors.affiliation: Departamento de Farmacología y Toxicología, Centro de Investigación y de Estudios Avanzados del I.P.N., México, D.F.
authors.forename: G
authors.initials: G
authors.lastname: Castañeda-Hernández
authors.forename: F J
authors.initials: FJ
authors.lastname: Flores-Murrieta
mesh_terms: D000818:Animals; D002316:Cardiotonic Agents / Q000037:antagonists & inhibitors* / Q000302:isolation & purification / Q000494:pharmacology; D004077:Digoxin / Q000494:pharmacology; D004347:Drug Interactions; D004789:Enzyme Activation; D006168:Guinea Pigs; D007700:Kinetics; D009124:Muscle Proteins / Q000037:antagonists & inhibitors*; D009206:Myocardium / Q000737:chemistry* / Q000201:enzymology; D010042:Ouabain / Q000378:metabolism / Q000494:pharmacology; D011189:Potassium Chloride / Q000494:pharmacology*; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors / Q000187:drug effects* / Q000378:metabolism; D014020:Tissue Extracts
chemical_list: D002316:Cardiotonic Agents; D009124:Muscle Proteins; D014020:Tissue Extracts; D010042:Ouabain; D011189:Potassium Chloride; D004077:Digoxin; D000254:Sodium-Potassium-Exchanging ATPase