title: Characterization of saccharide moiety in the electroplax sodium channel.
abstract: Carbohydrate chains on the large peptide of the voltage-sensitive sodium channel from Electrophorus electricus electroplax have been partially characterized by the lectin-blotting technique combined with digestion using three glucosidases: neuraminidase, endo-beta-N-acetylglucosaminidase H, and peptide: N-glycosidase F. The results show that both N-linked oligosaccharides and O-linked (mucin-type) oligosaccharides are present. In N-linked oligosaccharides, the results suggest the presence of complex- and hybrid-type oligosaccharides which contain bisecting N-acetylglucosamine(s), as well as the complex-type oligosaccharides with the alpha-Fuc-GlcNAc-(Asn) residue(s). In O-linked oligosaccharides, they must carry Gal beta1----3GalNAc- moieties which contain NeuNAc residues in the terminal.
authors.affiliation: Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Nakayama
authors.forename: S
authors.initials: S
authors.lastname: Yamamoto
authors.forename: Y
authors.initials: Y
authors.lastname: Hatanaka
authors.forename: T
authors.initials: T
authors.lastname: Hachisu
authors.forename: S
authors.initials: S
authors.lastname: Tsunasawa
authors.forename: F
authors.initials: F
authors.lastname: Sakiyama
authors.forename: Y
authors.initials: Y
authors.lastname: Kanaoka
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002236:Carbohydrate Conformation; D004593:Electrophorus; D006026:Glycoside Hydrolases / Q000494:pharmacology; D037102:Lectins; D009844:Oligosaccharides / Q000737:chemistry* / Q000302:isolation & purification; D037121:Plant Lectins; D015222:Sodium Channels / Q000737:chemistry* / Q000187:drug effects; D014909:Wheat Germ Agglutinins
chemical_list: D037102:Lectins; D009844:Oligosaccharides; D037121:Plant Lectins; C017707:Ricinus communis agglutinin-1; D015222:Sodium Channels; D014909:Wheat Germ Agglutinins; D006026:Glycoside Hydrolases