Analysis of the amino terminal presequence of the feline immunodeficiency virus glycoprotein: effect of deletions on the intracellular transport of gp95.
The envelope glycoprotein of feline immunodeficiency virus (FIV) consists of two noncovalently associated subunits, the surface glycoprotein (SU; gp95) and the transmembrane glycoprotein (TM; gp40). An unusual feature of the open reading frame (ORF) encoding the FIV glycoprotein is the presence of an unusually long amino terminal sequence (149 amino acids, "L" region or n-region of the signal sequence) preceding the predicted hydrophobic signal sequence. To examine the role of this n-region in the biosynthesis of gp95, the gene-encoding signal sequence and the surface glycoprotein (gp95) were expressed using recombinant vaccinia viruses. Glycoprotein mutants were constructed with 25, 42, 73, 102, and 147 amino acids removed from the n-region. Expression studies revealed that deletion of 25-102 amino acids did not appreciably effect the biosynthesis, intracellular transport, and release of gp95 from the cell surface. In contrast, removal of 147 of 149 amino acids resulted in the gp95 that was blocked in release from the cell. These results indicate that between 3 and 47 amino acids of the n-region are required for the proper biosynthesis, processing, and release of the FIV gp95 from infected cells.
Stephens
E B
EB
Department of Infectious Diseases, College of Veterinary Medicine, University of Florida, Gainesville 32611.
Butfiloski
E J
EJ
Monck
E
E
D001483:Base Sequence; D003001:Cloning, Molecular; D015686:Gene Products, env / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D016089:Immunodeficiency Virus, Feline / Q000235:genetics*; D008969:Molecular Sequence Data; D016296:Mutagenesis / Q000235:genetics; D016133:Polymerase Chain Reaction; D021382:Protein Sorting Signals / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D014616:Vaccinia virus / Q000235:genetics
D015686:Gene Products, env; D021382:Protein Sorting Signals; D011993:Recombinant Fusion Proteins