title: Functional nerve growth factor receptors on human B lymphocytes. Interaction with IL-2.
abstract: In addition to its neurotrophic activity, nerve growth factor (NGF) has been shown to interact with cells of the immune system. We have characterized the effects of NGF on human B cell proliferation and the regulation of NGF receptor expression on these cells. Nerve growth factor receptors were expressed on all tonsillar and peripheral blood B cells and this expression was increased upon activation of the cells. NGF augmented the mitogenic effect of the T-independent B cell mitogen, Staphylococcus aureus Cowan I strain, and provided a progression signal to competent B cells. The proliferative response was augmented when the progression signal provided by NGF was combined with that provided by IL-2 but not with IL-4. One effect of the interaction between NGF and IL-2 appears to occur at the receptor level, because each of these ligands increased the expression of the receptor for the other ligand, whereas IL-4 was without effect. These results demonstrate the expression of functional receptors on human B lymphocytes, the involvement of NGF in immuno-regulation, and indicate that NGF may act as a B cell growth factor.
authors.affiliation: Department of Pediatrics, National Jewish Center for Immunology and Respiratory Medicine, Denver, CO 80224.
authors.forename: C
authors.initials: C
authors.lastname: Brodie
authors.forename: E W
authors.initials: EW
authors.lastname: Gelfand
mesh_terms: D001402:B-Lymphocytes / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D007376:Interleukin-2 / Q000494:pharmacology; D015847:Interleukin-4 / Q000494:pharmacology; D008213:Lymphocyte Activation; D014066:Palatine Tonsil / Q000166:cytology; D011956:Receptors, Cell Surface / Q000378:metabolism*; D015375:Receptors, Interleukin-2 / Q000378:metabolism; D017475:Receptors, Nerve Growth Factor
chemical_list: D007376:Interleukin-2; D011956:Receptors, Cell Surface; D015375:Receptors, Interleukin-2; D017475:Receptors, Nerve Growth Factor; D015847:Interleukin-4