Epstein-Barr virus BCRF1 gene product (viral interleukin 10) inhibits superoxide anion production by human monocytes.
Due to its similar biological activities to interleukin 10 (IL-10), Epstein-Barr virus (EBV) BCRF1 gene product (viral IL-10: vIL-10) has recently been recognized as an analogue of authentic IL-10. Preincubation of human monocytes with vIL-10, like human IL-10, induced smaller amounts of interferon-gamma (IFN-gamma) mRNA in activated human peripheral blood mononuclear cells (PBMNCs) than nonpreincubation, indicating that vIL-10 acts principally on monocytes. Since the activation of monocytes and their generation of oxidative products are regulated by various cytokines, we examined the effects of vIL-10 on superoxide anion (O2-) production by human PBMNCs and monocytes. Not only PBMNCs but also monocytes preincubated with vIL-10 showed a smaller production of O2-. Inhibition was achieved in a dose-dependent fashion and increased gradually after incubation with vIL-10. Additions of IFN-gamma, macrophage colony-stimulating factor (M-CSF) or granulocyte-macrophage colony-stimulating factor (GM-CSF), which prime monocyte activation and induce O2- production, were also affected by the reciprocal effect of vIL-10. Thus, vIL-10 production by EBV-infected cells may be involved in the development of EBV-related disorders.
Niiro
H
H
First Department of Internal Medicine, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Otsuka
T
T
Abe
M
M
Satoh
H
H
Ogo
T
T
Nakano
T
T
Furukawa
Y
Y
Niho
Y
Y
D001483:Base Sequence; D004305:Dose-Response Relationship, Drug; D005814:Genes, Viral; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D004854:Herpesvirus 4, Human / Q000235:genetics*; D007371:Interferon-gamma / Q000378:metabolism / Q000494:pharmacology; D016753:Interleukin-10 / Q000235:genetics*; D016173:Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D008969:Molecular Sequence Data; D009000:Monocytes / Q000187:drug effects*; D013481:Superoxides / Q000037:antagonists & inhibitors*; D014764:Viral Proteins / Q000494:pharmacology*
C066460:BCRF1 protein, Human herpesvirus 4; D014764:Viral Proteins; D013481:Superoxides; D016753:Interleukin-10; D016173:Macrophage Colony-Stimulating Factor; D007371:Interferon-gamma; D016178:Granulocyte-Macrophage Colony-Stimulating Factor