title: Equivalence of a high-performance liquid chromatographic assay and a bioassay of azithromycin in human serum samples.
abstract: Two sensitive methods for the determination of the azalide antibiotic azithromycin in human serum were compared. High-performance liquid chromatography (HPLC) and a microbiological assay were simultaneously applied to 768 serum samples obtained in a clinical study. There was excellent agreement between the azithromycin concentrations measured by HPLC and by the bioassay. The correlation coefficient for the two methods was r2 = 0.96. The precision and the sensitivity of the methods were found to be very similar.
authors.affiliation: Department of Research and Development, Pfizer/Mack, Illertissen, Germany.
authors.forename: K D
authors.initials: KD
authors.lastname: Riedel
authors.forename: A
authors.initials: A
authors.lastname: Wildfeuer
authors.forename: H
authors.initials: H
authors.lastname: Laufen
authors.forename: T
authors.initials: T
authors.lastname: Zimmermann
mesh_terms: D017963:Azithromycin; D001681:Biological Assay; D002851:Chromatography, High Pressure Liquid; D004917:Erythromycin / Q000031:analogs & derivatives* / Q000097:blood / Q000493:pharmacokinetics; D006801:Humans
chemical_list: D004917:Erythromycin; D017963:Azithromycin