Cytochemical investigation of p-NPPase activity in rat cardiac muscle.
The cytochemical demonstration of the atrial cardiac myocyte pumping activity has been made by detecting p-nitrophenylphosphatase (NPPase), which is used by investigating of Na(+)-K(+)-ATPase and H(+)-K(+)-ATPase activities. The fine ultrastructural localization of these enzymes was studied using cytochemical methods with cerium as a capturing agent. Na(+)-K(+)-ATPase was localized on the atrial muscle cell plasma membrane, T-tubule membrane, endothelial cell nuclear membrane, and cardiac myocyte nuclear membrane. H(+)-K(+)-ATPase was localized on the atrial muscle cells plasma membrane, T-tubule membrane, and sarcoplasmatic reticulum. The present findings indicate that the transporting metabolic activity of the heart as an endocrine organ is realized by the interaction between p-NPPases.
Zinchuk
V S
VS
Department of Pathological Anatomy, Kiev Medical Institute, USSR.
D009597:4-Nitrophenylphosphatase / Q000032:analysis*; D000251:Adenosine Triphosphatases / Q000032:analysis*; D000818:Animals; D002462:Cell Membrane / Q000201:enzymology; D002563:Cerium / Q000737:chemistry; D017506:H(+)-K(+)-Exchanging ATPase; D008297:Male; D009206:Myocardium / Q000201:enzymology* / Q000648:ultrastructure; D009685:Nuclear Envelope / Q000201:enzymology; D051381:Rats; D011919:Rats, Inbred Strains; D012519:Sarcoplasmic Reticulum / Q000201:enzymology; D000254:Sodium-Potassium-Exchanging ATPase / Q000032:analysis*
D002563:Cerium; D009597:4-Nitrophenylphosphatase; D000251:Adenosine Triphosphatases; D017506:H(+)-K(+)-Exchanging ATPase; D000254:Sodium-Potassium-Exchanging ATPase