Antisense inhibition of gene expression: a tool for studying the role of NMYC in the growth and differentiation of neuroectoderm-derived cells.
To understand the role of individual genes in regulating biological processes, one must be able to interfere specifically with either their expression or function. While monoclonal antibodies have proven very useful in studying cell surface proteins, the specific inhibition of intracellular proteins in viable cells is a much more difficult problem. The goal of antisense technology is to develop small oligonucleotides, plasmids, or retroviral vectors that can be introduced easily into viable cells in order to inhibit gene products specifically. In this report, we will describe our use of antisense DNA and RNA to study the role of the NMYC proto-oncogene in neuroectodermal cell growth and differentiation.
Neckers
L M
LM
Tumor Cell Biology Section, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892.
Rosolen
A
A
Whitesell
L
L
D000818:Animals; D016373:DNA, Antisense / Q000494:pharmacology*; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D016259:Genes, myc; D006801:Humans; D009373:Neoplasms, Germ Cell and Embryonal / Q000235:genetics; D011506:Proteins / Q000037:antagonists & inhibitors; D000090063:Proto-Oncogene Mas; D016372:RNA, Antisense / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D016373:DNA, Antisense; C000715470:MAS1 protein, human; D011506:Proteins; D000090063:Proto-Oncogene Mas; D016372:RNA, Antisense