Effects of N-methyl-D-aspartate on quisqualate-stimulated phosphoinositide hydrolysis in slices of kitten striate cortex.
Stimulation of phosphoinositide (PI) hydrolysis by excitatory amino acids (EAAs) was studied in coronal slices of kitten visual cortex. Coincubation with N-methyl-D-aspartate (NMDA) markedly reduced the stimulation by quisqualate, however, this inhibition developed with a latency of > 10 min and occurred even when the NMDA exposure preceded, but did not overlap with, incubation in quisqualate. This time-course of NMDA inhibition of EAA-stimulated PI turnover places new constraints on its possible mechanism of inhibition.
Bohner
A P
AP
Department of Neuroscience, Brown University, Providence 02912.
Dudek
S M
SM
Bear
M F
MF
D000818:Animals; D002415:Cats; D006868:Hydrolysis; D066298:In Vitro Techniques; D016202:N-Methylaspartate / Q000494:pharmacology*; D010716:Phosphatidylinositols / Q000378:metabolism*; D016318:Quisqualic Acid / Q000037:antagonists & inhibitors*; D014793:Visual Cortex / Q000187:drug effects* / Q000378:metabolism
D010716:Phosphatidylinositols; D016202:N-Methylaspartate; D016318:Quisqualic Acid