Evaluation of different immunization protocols with P. brasiliensis antigens in guinea pigs.
The objective of the present study was to develop an efficient and reproducible protocol of immunization of guinea pigs with P. brasiliensis antigens as an animal model for future studies of protective immunity mechanisms. We tested three different antigens (particulate, soluble and combined) and six protocols in the presence and absence of Freund's complete adjuvant and with different numbers of immunizing doses and variable length of time between the last immunizing dose and challenge. The efficacy of the immunizing protocol was evaluated by measuring the humoral and cellular anti-P. brasiliensis immune response of the animals, using immunodiffusion, skin test and macrophage migration inhibition test. It was observed that: 1. Three immunizing doses of the antigens induced a more marked response than two doses; 2. The highest immune response was obtained with the use of Freund's complete adjuvant; 3. Animals challenged a long time (week 6) after the last immunizing dose showed good anti-P. brasiliensis immune response; 4. The particulate antigen induced the lowest immune response. The soluble and the combined antigens were equally efficient in raising good humoral and cellular anti-P. brasiliensis immune response.
Kamegasawa
A
A
Department of Ophthalmology, Faculty of Medicine, UNESP, Botucatu, Brasil.
Iwasso
M T
MT
Viero
R
R
Franco
M
M
D000818:Animals; D000917:Antibody Formation; D000946:Antigens, Fungal / Q000008:administration & dosage / Q000276:immunology* / Q000302:isolation & purification; D004195:Disease Models, Animal; D006168:Guinea Pigs; D007111:Immunity, Cellular; D007114:Immunization / Q000379:methods*; D008297:Male; D010228:Paracoccidioides / Q000276:immunology*; D010229:Paracoccidioidomycosis / Q000209:etiology / Q000276:immunology / Q000473:pathology; D012867:Skin / Q000276:immunology / Q000473:pathology; D012995:Solubility; D013997:Time Factors
D000946:Antigens, Fungal