title: Testosterone destroys the transcortin-receptor complex.
abstract: Dissociation of the complex of transcortin receptor with immobilized transcortin in the presence of 10(-5) M testosterone has been shown with the use of affinity chromatography on transcortin-Sepharose. The specificity of this effect is confirmed by its abrogation in the presence of cortisol. The testosterone effect has been used for the elution of transcortin receptor from affinity column. The receptor retained transcortin-binding capacity after the elution and removal of testosterone. Characteristics of the receptor obtained by testosterone elution were identical with those of the transcortin eluted preparation.
authors.affiliation: Institute of Bioorganic Chemistry, Byelorussian Academy of Sciences, Minsk, Belarus.
authors.forename: S A
authors.initials: SA
authors.lastname: Krupenko
authors.forename: O A
authors.initials: OA
authors.lastname: Strel'chyonok
mesh_terms: D002462:Cell Membrane / Q000378:metabolism; D002846:Chromatography, Affinity / Q000379:methods; D003656:Decidua; D004591:Electrophoresis, Polyacrylamide Gel; D004717:Endometrium / Q000378:metabolism; D005260:Female; D006801:Humans; D007525:Isoelectric Focusing; D008970:Molecular Weight; D011247:Pregnancy; D011956:Receptors, Cell Surface / Q000187:drug effects / Q000302:isolation & purification / Q000378:metabolism*; D015843:Serpins; D013739:Testosterone / Q000494:pharmacology*; D014156:Transcortin / Q000378:metabolism*
chemical_list: D011956:Receptors, Cell Surface; C534935:SERPINA6 protein, human; D015843:Serpins; D013739:Testosterone; D014156:Transcortin