A noninvasive assessment of myocardial oxygen tension: 19F NMR spectroscopy of sequestered perfluorocarbon emulsion.
Fluorine NMR spectroscopy of sequestered perfluorocarbon emulsion has been used to measure myocardial oxygen tension. This novel application provides a rapid noninvasive assessment of changes in oxygen tension in response to ischemia and reperfusion. Rats were predosed with Oxypherol-ET (emulsion of perfluorotributylamine). Following vascular clearance of the emulsion the heart was excised and perfused using the Langendorff retrograde technique. 19F spin-lattice relaxation time measurements provided an accurate estimate of myocardial pO2. Using a two-point determination with a time resolution of 1 s, the loss of oxygen was found to be complete within 40 s of the onset of global ischemia. The fall in oxygen tension correlated closely with an observed loss of ventricular pressure. Magnetic resonance imaging showed that perfluorocarbon was distributed throughout the heart; thus, this reporter molecule provides a global measurement of oxygen tension.
Mason
R P
RP
Department of Radiology, University of Texas Southwestern Medical Center, Dallas 75235.
Jeffrey
F M
FM
Malloy
C R
CR
Babcock
E E
EE
Antich
P P
PP
D000818:Animals; D001802:Blood Substitutes; D004578:Electron Spin Resonance Spectroscopy; D004655:Emulsions; D005461:Fluorine; D005466:Fluorocarbons; D008279:Magnetic Resonance Imaging; D009682:Magnetic Resonance Spectroscopy; D017202:Myocardial Ischemia / Q000378:metabolism; D015425:Myocardial Reperfusion; D009206:Myocardium / Q000378:metabolism*; D010101:Oxygen Consumption; D051381:Rats; D017207:Rats, Sprague-Dawley
D001802:Blood Substitutes; D004655:Emulsions; D005466:Fluorocarbons; D005461:Fluorine; C008807:perfluorotributylamine