Inhibition of cell cycle progression by antisense oligodeoxynucleotides.
We have used the antisense strategy to study the role of certain genes in cell cycle progression. In particular, we used antisense oligodeoxynucleotides to study: (1) the role of the IGF-1 receptor in the control of cell proliferation; and (2) the sequence of gene expression during the cell cycle. Our results can be summarized as follows: (1) the activation of the IGF-1 receptor by its ligand, IGF-1, is an obligatory step in the proliferation of fibroblasts and hemopoietic cells; and (2) the expression of DNA synthesis genes, such as PCNA, DNA polymerase alpha, and cdc2, is dependent on the expression of previous genes. A tentative temporal order is: c-myc > c-myb > IGF-1 receptor > DNA synthesis genes.
Baserga
R
R
Jefferson Cancer Institute, Jefferson Medical College, Philadelphia, Pennsylvania 19107-5541.
Reiss
K
K
Alder
H
H
Pietrzkowski
Z
Z
Surmacz
E
E
D000818:Animals; D002453:Cell Cycle / Q000187:drug effects*; D002478:Cells, Cultured; D016259:Genes, myc / Q000235:genetics; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000187:drug effects; D008957:Models, Genetic; D009838:Oligodeoxyribonucleotides / Q000494:pharmacology*; D016376:Oligonucleotides, Antisense / Q000494:pharmacology*; D010835:Phytohemagglutinins / Q000494:pharmacology; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D017526:Receptor, IGF Type 1 / Q000235:genetics*
D009838:Oligodeoxyribonucleotides; D016376:Oligonucleotides, Antisense; D010835:Phytohemagglutinins; D012333:RNA, Messenger; D017526:Receptor, IGF Type 1