Possible involvement of superoxide anion in the induction of cyanide-resistant respiration in Hansenula anomala.
A chemiluminescence study showed that Qi site inhibitors such as antimycin A induce O2- generation in respiring cyanide-sensitive mitochondria from the yeast, Hansenula anomala. The O2- generation was suppressed by radical scavengers such as flavone, butylated hydroxyanisole, and Co0. Induction of cyanide-resistant respiration in H. anomala cells by Qi site inhibitors was also inhibited by these radical scavengers. Furthermore, antimycin A-induced synthesis of the mitochondrial 36-kDa protein, which is thought to be the alternative oxidase functional in the cyanide-resistant respiratory pathway, was abolished by the addition of flavone. These observations suggest that O2- is somehow involved in the induction of cyanide-resistant respiration.
Minagawa
N
N
Department of Biochemistry, Niigata College of Pharmacy, Japan.
Koga
S
S
Nakano
M
M
Sakajo
S
S
Yoshimoto
A
A
D000838:Anions; D000968:Antimycin A / Q000494:pharmacology; D000975:Antioxidants / Q000494:pharmacology; D003486:Cyanides / Q000494:pharmacology*; D044950:Flavanones; D047309:Flavones; D005419:Flavonoids / Q000494:pharmacology; D016166:Free Radical Scavengers; D049449:Luminescence; D010101:Oxygen Consumption / Q000187:drug effects*; D010843:Pichia / Q000187:drug effects / Q000378:metabolism*; D013481:Superoxides / Q000378:metabolism*
D000838:Anions; D000975:Antioxidants; D003486:Cyanides; D044950:Flavanones; D047309:Flavones; D005419:Flavonoids; D016166:Free Radical Scavengers; D013481:Superoxides; D000968:Antimycin A; C043562:flavone; C028610:flavanone