Glycinergic ligands modulate the rate of phosphorylation of the glycine receptor by protein kinase C.
The alpha subunit of the glycine receptor purified from rat spinal cord is rapidly and specifically phosphorylated by protein kinase C (Ruiz-Gómez et al., (1991) J. Biol. Chem. 266, 559-566). We report here that the rate of phosphorylation of the glycine receptor by this kinase is higher in the presence of agonists (glycine, beta-alanine) than in the presence of antagonists (strychnine, RU-5135). These results suggest that activated glycine receptors would be a preferential target for functional regulation through phosphorylation mechanisms.
Vaello
M L
ML
Centro de Biología Molecular Severo Ochoa (CSIC-UAM), Universidad Autònoma, Madrid, Spain.
Ruiz-Gómez
A
A
Mayor
F
F
D000409:Alanine / Q000494:pharmacology*; D000731:Androstanes / Q000494:pharmacology; D000818:Animals; D001378:Azasteroids / Q000494:pharmacology; D001921:Brain / Q000201:enzymology; D005998:Glycine / Q000378:metabolism / Q000494:pharmacology*; D007700:Kinetics; D008024:Ligands; D046911:Macromolecular Substances; D010766:Phosphorylation; D011493:Protein Kinase C / Q000302:isolation & purification / Q000378:metabolism*; D051381:Rats; D018009:Receptors, Glycine; D017981:Receptors, Neurotransmitter / Q000187:drug effects / Q000302:isolation & purification / Q000378:metabolism*; D013116:Spinal Cord / Q000378:metabolism; D013331:Strychnine / Q000378:metabolism / Q000494:pharmacology*
D000731:Androstanes; D001378:Azasteroids; D008024:Ligands; D046911:Macromolecular Substances; D018009:Receptors, Glycine; D017981:Receptors, Neurotransmitter; D011493:Protein Kinase C; D013331:Strychnine; D000409:Alanine; C032061:RU 5135; D005998:Glycine