title: GABAA antagonists reveal binding sites for [35S]TBPS in cerebellar granular cell layer.
abstract: Autoradiography revealed picrotoxin-sensitive [35S]TBPS ([35S]t-butylbicyclophosphorothionate) binding sites in the cerebellar granule cell layer only in the presence of GABAA receptor antagonists. However, under the same conditions, the binding was decreased in other brain regions, including the cerebellar molecular cell layer. Thus, the convulsant binding sites at GABAA receptors in the granule cell layer are highly sensitive to down-regulation by GABA, which may be due to a unique combination of receptor subunits.
authors.affiliation: Laboratory of Molecular Neuroendocrinology, ZMBH, University of Heidelberg, F.R.G.
authors.forename: E R
authors.initials: ER
authors.lastname: Korpi
authors.forename: H
authors.initials: H
authors.lastname: Lüddens
authors.forename: P H
authors.initials: PH
authors.lastname: Seeburg
mesh_terms: D000731:Androstanes / Q000494:pharmacology; D000818:Animals; D001345:Autoradiography; D001378:Azasteroids / Q000494:pharmacology; D001665:Binding Sites; D001921:Brain / Q000378:metabolism / Q000648:ultrastructure; D001643:Bridged Bicyclo Compounds / Q000378:metabolism*; D019086:Bridged Bicyclo Compounds, Heterocyclic; D002525:Cerebellar Cortex / Q000166:cytology / Q000378:metabolism / Q000648:ultrastructure*; D003292:Convulsants / Q000378:metabolism; D018756:GABA Antagonists; D058787:GABA-A Receptor Antagonists; D008297:Male; D011724:Pyridazines / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D013462:Sulfur Radioisotopes
chemical_list: D000731:Androstanes; D001378:Azasteroids; D001643:Bridged Bicyclo Compounds; D019086:Bridged Bicyclo Compounds, Heterocyclic; D003292:Convulsants; D018756:GABA Antagonists; D058787:GABA-A Receptor Antagonists; D011724:Pyridazines; D013462:Sulfur Radioisotopes; C037476:tert-butylbicyclophosphorothionate; C049853:gabazine; C032061:RU 5135