title: Effects of mucoid and non-mucoid Pseudomonas aeruginosa isolates from cystic fibrosis patients on inflammatory mediator release from human polymorphonuclear granulocytes and rat mast cells.
abstract: Mucoid Pseudomonas aeruginosa causing chronic bronchopulmonary infection in cystic fibrosis (CF) patients may interfere with host defence mechanisms. We investigated 13 P. aeruginosa strains isolated from sputa of CF patients with regard to the induction or modulation of inflammatory mediator release from human neutrophils (PMN) and rat mast cells. The effects of mucoid as compared to non-mucoid bacteria were studied using a mucoid strain and its non-mucoid revertant. The release of leukotrienes (LT) and histamine in response to the majority of the CF strains was insignificant. However, preincubation of PMN with P. aeruginosa caused a dose-dependent decrease (50-95%) of LTB4 and LTC4 generation and LTB4 metabolism induced by the Ca(2+)-ionophore A23187 or opsonized zymosan (ZX) (P less than 0.001). The mucoid strains caused a three- to 10-fold higher impairment of LTB4 release (P less than 0.05) and a concomitant down-regulation of LTB4 receptors on neutrophils. Inhibitory effects were also obtained for mucoid and non-mucoid bacteria when the phorbol-ester or the Ca(2+)-ionophore induced luminol enhanced chemiluminescence response (P less than 0.001) or the histamine release from rat peritoneal mast cells (P less than 0.01) was studied. The bacteria-cell contact with non-mucoid strains was associated with an increased Ca2+ influx into PMN, whereas mucoid bacteria had no effect. In addition, a protein kinase C-dependent decrease of the C3bi receptor was suppressed by the mucoid--and less effectively--by the non-mucoid strain. The results suggest that the impairment of the phagocytic and inflammatory system may contribute to the pathogenesis and persistence of mucoid P. aeruginosa infection in CF.
authors.affiliation: Institute of Microbiology and Immunology, Ruhr University, Bochum, Germany.
authors.forename: P
authors.initials: P
authors.lastname: Friedl
authors.forename: B
authors.initials: B
authors.lastname: König
authors.forename: W
authors.initials: W
authors.lastname: König
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D003550:Cystic Fibrosis / Q000382:microbiology*; D006632:Histamine / Q000378:metabolism; D006801:Humans; D015289:Leukotrienes / Q000096:biosynthesis; D008407:Mast Cells / Q000276:immunology*; D009504:Neutrophils / Q000276:immunology*; D010100:Oxygen / Q000097:blood; D011552:Pseudomonas Infections / Q000276:immunology*; D051381:Rats; D011951:Receptors, Complement / Q000032:analysis; D017463:Receptors, Complement 3b; D011971:Receptors, Immunologic / Q000032:analysis; D018102:Receptors, Leukotriene B4; D013183:Sputum / Q000382:microbiology*
chemical_list: D015289:Leukotrienes; D011951:Receptors, Complement; D017463:Receptors, Complement 3b; D011971:Receptors, Immunologic; D018102:Receptors, Leukotriene B4; D006632:Histamine; D010100:Oxygen; D002118:Calcium