[Pyrophosphate-dependent inactivation of tyrosyl-tRNA synthetase during aminoacylation of heterologous tRNA].
It has been shown that heterologous aminoacylation of tRNA by tyrosyl-tRNA synthetase leads to inactivation of the enzyme. Inorganic pyrophosphatase prevents the inactivation and increases the enzyme activity and aminoacylation level in a heterologous system. A putative inactivation mechanism is discussed.
Khvorova
A M
AM
Motorin
Iu A
IuA
Vol'fson
A D
AD
D000215:Acylation; D000588:Amines / Q000737:chemistry; D000818:Animals; D002417:Cattle; D011756:Diphosphates / Q000378:metabolism*; D008099:Liver / Q000201:enzymology; D012343:RNA, Transfer / Q000378:metabolism*; D011817:Rabbits; D014448:Tyrosine-tRNA Ligase / Q000037:antagonists & inhibitors* / Q000378:metabolism
D000588:Amines; D011756:Diphosphates; D012343:RNA, Transfer; D014448:Tyrosine-tRNA Ligase
Pirofosfatzavisimaia inaktivatsiia tirozil-tRNK sintetazy pri aminoatsilirovanii geterologichnoĭ tRNK.