Physical mapping of loci in the distal half of the short arm of the human X chromosome: implications for the spreading of X-chromosome inactivation.
The relative order of 11 loci in the distal half of the short arm of the human X chromosome was examined using a panel of somatic cell hybrids containing structurally rearranged X chromosomes. The results show that the gene for phosphoribosylpyrophosphate synthetase 2 (PRPS2) is located between ZFX (zinc finger protein, X-linked) and STS (steroid sulfatase). The results also confirm the localization of ZFX distal to POLA (alpha-DNA polymerase). Previous studies have shown that STS and ZFX escape X-inactivation whereas POLA undergoes inactivation. Evaluation of PRPS2 expression in somatic cell hybrids containing inactive human X chromosomes showed that PRPS2 undergoes X-inactivation. These results provide further evidence for interspersion of loci that do and do not undergo X-inactivation on the human X chromosome.
Wang
J C
JC
Division of Medical Genetics, Harbor-UCLA Medical Center, Torrance 90509.
Passage
M B
MB
Ellison
J
J
Becker
M A
MA
Yen
P H
PH
Shapiro
L J
LJ
Mohandas
T K
TK
D001192:Arylsulfatases / Q000235:genetics*; D015152:Blotting, Northern; D015139:Blotting, Southern; D004257:DNA Polymerase II / Q000235:genetics*; D015342:DNA Probes / Q000235:genetics; D004303:Dosage Compensation, Genetic; D005260:Female; D006801:Humans; D006822:Hybrid Cells; D012268:Ribose-Phosphate Pyrophosphokinase / Q000235:genetics*; D043266:Steryl-Sulfatase; D014960:X Chromosome; D016335:Zinc Fingers / Q000235:genetics
D015342:DNA Probes; D012268:Ribose-Phosphate Pyrophosphokinase; D004257:DNA Polymerase II; D001192:Arylsulfatases; D043266:Steryl-Sulfatase