Gelsolin binds to polymeric actin at a low rate.
Association of gelsolin with actin filament subunits was investigated by the decrease of the fluorescence intensity of a 7-nitro-2-oxa-1,3-diazole (NBD) label covalently linked to gelsolin. The rate constant of this reaction was found to be 4 x 10(3) M-1 s-1. Binding of NBD-labeled gelsolin to monomeric actin proceeds at a similar low rate. The rate of association of gelsolin that was unmodified to actin filament subunits was estimated too. Unmodified gelsolin was added to a mixture of actin filaments and actin-DNase I complex. The fractions of gelsolin that bound to actin filament subunits or to actin-DNase I complex depended on the relative rates of these two competing reactions. In this way it was possible to estimate the rate constant of association of unmodified gelsolin with actin filament subunits (2 x 10(4) M-1 s-1). Thus, gelsolin associates with actin filament subunits at a rate that is considerably slower than diffusion-controlled and similar to the rate of binding of gelsolin to monomeric actin.
Schoepper
B
B
Institute of Physiological Chemistry, Ruhr-University Bochum, Federal Republic of Germany.
Wegner
A
A
D000199:Actins / Q000302:isolation & purification / Q000378:metabolism*; D000818:Animals; D002135:Calcium-Binding Proteins / Q000302:isolation & purification / Q000378:metabolism*; D002645:Chickens; D003850:Deoxyribonuclease I / Q000378:metabolism; D018260:Gelsolin; D005895:Gizzard, Avian; D007700:Kinetics; D046911:Macromolecular Substances; D008433:Mathematics; D008840:Microfilament Proteins / Q000302:isolation & purification / Q000378:metabolism*; D009130:Muscle, Smooth / Q000378:metabolism; D009132:Muscles / Q000378:metabolism; D011817:Rabbits
D000199:Actins; D002135:Calcium-Binding Proteins; D018260:Gelsolin; D046911:Macromolecular Substances; D008840:Microfilament Proteins; D003850:Deoxyribonuclease I