title: On employing Leishmania promastigotes for systemic lupus erythematosus diagnosis.
abstract: Based on having definite double stranded DNA determinants in the kinetoplast, it was attempted to use Leishmania promastigotes as an IFAT substrate for the detection of serum anti ds-DNA autoantibodies which are the prominent diagnostic marker of SLE. Serological inspection of different Leishmania preparations revealed that Leishmania is not analogous to Crithidia lucilia as an appropriate ds-DNA substrate. It seems likely that the species-specific molecular characters of the kinetoplast-DNA are influencing the reaction. The issue is an evidence of the distinct differentiation of the K-DNA antigenic determinants in haemoflagellates.
authors.affiliation: Department of Parasitology, Faculty of Medicine, Ain Shams University, Cairo, Egypt.
authors.forename: M E
authors.initials: ME
authors.lastname: Azab
authors.forename: M A
authors.initials: MA
authors.lastname: el Mawla
authors.forename: M M
authors.initials: MM
authors.lastname: Abbas
mesh_terms: D000818:Animals; D000974:Antibodies, Antinuclear / Q000032:analysis*; D004270:DNA, Circular / Q000276:immunology; D018105:DNA, Kinetoplast; D016054:DNA, Protozoan / Q000276:immunology; D006801:Humans; D007891:Leishmania / Q000235:genetics* / Q000276:immunology; D008180:Lupus Erythematosus, Systemic / Q000175:diagnosis*; D013045:Species Specificity
chemical_list: D000974:Antibodies, Antinuclear; D004270:DNA, Circular; D018105:DNA, Kinetoplast; D016054:DNA, Protozoan