title: Adhesion of 8701-BC breast cancer cells to type V collagen and 67 kDa receptor.
abstract: Ductal infiltration carcinomas (d.i.c.) of the breast are potentially highly metastatic tumours, associated with drastic alterations of the architecture and molecular composition of the extracellular matrix at the tumour-host interface. 8701-BC, a recently characterized cell line, isolated from primary d.i.c., was used to study different aspects of tumor cell-substratum interactions. Since type V collagen deposition is augmented in d.i.c. we have examined the ability of 8701-BC cells to interact with this collagen species. We have found that cell binding to type V collagen was mediated by protein homologous to the 67 kDa laminin receptor (67-R). This conclusion is substantiated by the following observations: (a) a major band having an apparent molecular mass of 67 kDa and immunoreactive to the anti-67 R antibody was detectable by SDS-PAGE of the membrane proteins; (b) the antibody inhibited cellular adhesion to type V collagen in a dose-dependent way; (c) membrane proteins purified by affinity chromatography on type V collagen were immunoreactive to anti-67 R antibody, but not to anti-VLA1, VLA2 and VLA3 integrin antibodies. This receptor appears to have prominent carbohydrate-binding properties, since lactose competes with cell adhesion to type V collagen.
authors.affiliation: Istituto di Istologia ed Embriologia, Università di Palermo, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Minafra
authors.forename: C
authors.initials: C
authors.lastname: Luparello
authors.forename: I
authors.initials: I
authors.lastname: Pucci-Minafra
authors.forename: M E
authors.initials: ME
authors.lastname: Sobel
authors.forename: S
authors.initials: S
authors.lastname: Garbisa
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D002846:Chromatography, Affinity; D003094:Collagen / Q000378:metabolism*; D007785:Lactose / Q000502:physiology; D008325:Mammary Neoplasms, Experimental / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000096:biosynthesis*; D038981:Receptors, Collagen; D014407:Tumor Cells, Cultured
chemical_list: D011956:Receptors, Cell Surface; D038981:Receptors, Collagen; D003094:Collagen; D007785:Lactose