title: [Effects of beta-agkistrodotoxin on synaptic transmission in toad sympathetic ganglia].
abstract: The effects of beta-agkistrodotoxin (beta-AgTX) on synaptic transmission of the toad sympathetic ganglia were investigated by intracellular recording techniques. Superfusion of beta-AgTX (30 microgram/ml, 5-15 min) reversibly inhibited the cholinergic fast excitatory postsynaptic potential (f-EPSP, n = 16) and the fast components of acetylcholine (ACh) potential induced by micropressure administration of ACh (n = 24). Comparison of beta-AgTX effect in the same cell group showed significantly different inhibition rates on f-EPSP (77.2 +/- 27.7%) and ACh potential (25.5 +/- 17.5%) (n = 6, P less than 0.01, F test). During application of beta-AgTX (30 or 50 micrograms/ml) for 15-30 min, no detectable change was found in non-cholinergic late slow EPSPs (n = 22). The results suggest that beta-AgTX selectively inhibits the cholinergic transmission of the toad sympathetic ganglia by both presynaptic and postsynaptic mechanism.
authors.affiliation: Cell Electrophysiology Laboratory, Wannan Medical College, Wuhu.
authors.forename: X T
authors.initials: XT
authors.lastname: Jiang
authors.forename: W D
authors.initials: WD
authors.lastname: Li
authors.forename: Z G
authors.initials: ZG
authors.lastname: Jiang
authors.forename: M Y
authors.initials: MY
authors.lastname: Wang
authors.forename: K
authors.initials: K
authors.lastname: Xu
authors.forename: J K
authors.initials: JK
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002023:Bufo bufo; D003435:Crotalid Venoms / Q000494:pharmacology*; D005260:Female; D005728:Ganglia, Sympathetic / Q000187:drug effects*; D008297:Male; D008839:Microelectrodes; D009498:Neurotoxins / Q000494:pharmacology*; D013569:Synapses / Q000187:drug effects; D009435:Synaptic Transmission / Q000187:drug effects*
chemical_list: D003435:Crotalid Venoms; D009498:Neurotoxins; C070121:beta-agkistrodotoxin