Polyamines enhance calcium mobilization in fMet-Leu-Phe-stimulated phagocytes.
Spermidine and putrescine (50 microM-1 mM), found in exudates from infection sites, significantly enhance fMet-Leu-Phe-induced Ca2+ mobilization in differentiated HL-60 cells and polymorphonuclear leukocytes (PMNs) by delaying the return to basal cytosolic Ca2+ levels. This enhancement by polyamines is associated with inhibition of Ca2+ efflux across the plasma membrane. In parallel with their effects on Ca2+ signaling, polyamines also significantly prolong the kinetics of fMet-Leu-Phe-induced protein kinase C translocation. Thus, polyamines may play a novel role in modulating regulatory events in phagocytes.
Walters
J D
JD
Department of Periodontology, College of Dentistry, Ohio State University, Columbus 43210.
Sorboro
D M
DM
Chapman
K J
KJ
D002118:Calcium / Q000378:metabolism*; D015220:Calcium Channels / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology*; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism*; D010586:Phagocytes / Q000187:drug effects / Q000378:metabolism*; D011700:Putrescine / Q000502:physiology*; D013095:Spermidine / Q000502:physiology*; D014407:Tumor Cells, Cultured
D015220:Calcium Channels; D009240:N-Formylmethionine Leucyl-Phenylalanine; D002118:Calcium; D013095:Spermidine; D011700:Putrescine