title: Structure of the Drosophila melanogaster gene encoding cyclin A.
abstract: A P element (PE)-induced Drosophila melanogaster mutation, hari, affects the formation of the bristle mechanosensory organ in the adult fly. In this mutation, the site of PE insertion is in the first intron of the gene (CycA) encoding cyclin A (CycA). In order to analyze the hari mutant at the molecular level, we cloned and sequenced the cDNA and genomic DNA encoding CycA. CycA has seven exons and six introns, and its transcription unit spans 6 kb. All exon-intron junctions are compatible with the GT/AG consensus. Results of primer extension analysis and RNase protection assay indicate that CycA has major and minor transcription start points (tsp). To our knowledge, this is the first report on the CycA genomic sequence from a multicellular organism.
authors.affiliation: Laboratory of Cell Biology, Mitsubishi Kasei Institute of Life Sciences, Tokyo, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Takahisa
authors.forename: S
authors.initials: S
authors.lastname: Togashi
authors.forename: R
authors.initials: R
authors.lastname: Ueda
authors.forename: M
authors.initials: M
authors.lastname: Mikuni
authors.forename: S
authors.initials: S
authors.lastname: Tsurumura
authors.forename: K
authors.initials: K
authors.lastname: Kondo
authors.forename: T
authors.initials: T
authors.lastname: Miyake
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D016213:Cyclins / Q000235:genetics*; D004247:DNA / Q000235:genetics; D004251:DNA Transposable Elements; D004331:Drosophila melanogaster / Q000235:genetics*; D005091:Exons; D017344:Genes, Insect; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D014158:Transcription, Genetic
chemical_list: D016213:Cyclins; D004251:DNA Transposable Elements; D004247:DNA