title: Comparative clinical and microbiological effects of topical subgingival application of metronidazole 25% dental gel and scaling in the treatment of adult periodontitis.
abstract: The aim of the study was to compare the clinical and microbiological effects of topical application of a metronidazole gel and a single session of subgingival scaling in the treatment of adult periodontitis. An open, randomized controlled clinical study design was employed. Each of 24 subjects received the 2 treatments simultaneously each in 2 randomly selected quadrants of the dentition. The metronidazole, 25% gel was applied subgingivally on days 0 and 7. Scaling was carried out in one quadrant on day 0 and in one quadrant on day 7. Clinical and microbiological examinations were carried out before treatment and on days 21, 49, 91, 133, and 175 of the experimental period. The microbiological analyses included determination of total anaerobically cultivable bacteria, and relative proportions of anaerobes, aerobes, black-pigmented anaerobic Gram negative rods, Actinobacillus actinomycetemcomitans, streptococci, and spirochetes. Both treatments were effective in reducing probing pocket depth and bleeding on probing. Metronidazole tended to be a little better than scaling during the study period and the clinical effects of both treatments persisted during the whole 6 months observation period. Local metronidazole treatment induced a significant and long-lasting shift in the subgingival flora towards a composition more compatible with health and comparable to that obtained by mechanical debridement. Proportions of black pigmented anaerobes including Prevotella intermedia, and the number of spirochetes were significantly reduced after both treatments with a concomitant increase in the proportions of streptococci. While scaling resulted in a statistically significant increase in the proportion of A. actinomycetemcomitans, this was avoided after metronidazole treatment.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Oral Biology, Royal Dental College, Aarhus, Denmark.
authors.forename: V
authors.initials: V
authors.lastname: Pedrazzoli
authors.forename: M
authors.initials: M
authors.lastname: Kilian
authors.forename: T
authors.initials: T
authors.lastname: Karring
mesh_terms: D000328:Adult; D000368:Aged; D016976:Aggregatibacter actinomycetemcomitans / Q000187:drug effects / Q000302:isolation & purification; D001419:Bacteria / Q000187:drug effects / Q000302:isolation & purification*; D001439:Bacteroides / Q000187:drug effects / Q000302:isolation & purification; D003692:Delayed-Action Preparations; D003773:Dental Plaque / Q000382:microbiology; D012534:Dental Scaling; D004343:Drug Implants; D005260:Female; D005500:Follow-Up Studies; D005782:Gels; D005884:Gingival Hemorrhage / Q000188:drug therapy / Q000382:microbiology / Q000628:therapy; D006801:Humans; D008297:Male; D008795:Metronidazole / Q000008:administration & dosage / Q000627:therapeutic use*; D008875:Middle Aged; D010514:Periodontal Pocket / Q000188:drug therapy / Q000382:microbiology / Q000628:therapy; D010518:Periodontitis / Q000188:drug therapy* / Q000382:microbiology / Q000628:therapy*; D016966:Porphyromonas gingivalis / Q000187:drug effects / Q000302:isolation & purification; D013291:Streptococcus / Q000187:drug effects / Q000302:isolation & purification
chemical_list: D003692:Delayed-Action Preparations; D004343:Drug Implants; D005782:Gels; D008795:Metronidazole