Development and characterization of a model of eosinophil-mediated cardiomyopathy in rats infected with Toxocara canis.
This study begins testing the hypothesis that a causal relationship exists between hypereosinophilia and cardiac dysfunction. Rats infected with the nematode Toxocara canis develop marked hypereosinophilia, with peak blood eosinophil levels of approximately 3,500 eosinophils/mm3 whole blood observed approximately 14 days postinfection. Corresponding to the increase in blood eosinophils of infected animals was a decrease in cardiac performance. By 14 days postinfection, cardiac work had declined approximately 25% while negative first derivative of pressure (-dP/dt) fell approximately 10%. As the extent of hypereosinophilia declined from a peak of approximately 3,500 eosinophils/mm3 whole blood to a new steady state of approximately 1,000 eosinophils/mm3, the degree of cardiac dysfunction also was reduced. Cardiac work was 10-15% less in rats 28-42 days postinfection while -dP/dt was 5% depressed in these animals. Myocardial dysfunction was also observed following exposure of perfused hearts obtained from uninfected rats to buffer containing activated eosinophils. The hearts exposed to activated eosinophils exhibited marked histological alterations, characterized by distention of the intermyocyte space, increased pericapillary space, and focal losses of striated staining pattern. These changes were associated with the accumulation of eosinophils within the myocardium, as evidenced by the cytochemical demonstration of eosinophil peroxidase activity within the heart. The data support the hypothesis that hypereosinophilia can lead to cardiac dysfunction.
Schaffer
S W
SW
Department of Pharmacology, College of Medicine, University of South Alabama, Mobile 36688.
Dimayuga
E R
ER
Kayes
S G
SG
D000818:Animals; D009202:Cardiomyopathies / Q000097:blood / Q000209:etiology* / Q000503:physiopathology; D002452:Cell Count; D004804:Eosinophils / Q000187:drug effects / Q000473:pathology / Q000502:physiology*; D006651:Histocytochemistry; D066298:In Vitro Techniques; D008297:Male; D009200:Myocardial Contraction / Q000187:drug effects; D009206:Myocardium / Q000201:enzymology; D010477:Perfusion; D009195:Peroxidase / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014120:Toxocariasis / Q000150:complications* / Q000503:physiopathology
D009195:Peroxidase; D013755:Tetradecanoylphorbol Acetate