Multianalytic study on cervical HPV lesions.
Nowadays new sophisticated techniques of molecular biology based on the principles of hybridization between nucleic acids, allow a correct diagnosis of genital HPV infection. In the present paper, beside traditional diagnostic methods, we used In Situ Hybridization (ISH) and Polymerase Chain Reaction (PCR) to detect the presence of HPV types 6, 11, 16, 18, 31 and 33. We tested ten patients affected by cervical lesions of high histological atypias associated with HPV, who underwent surgical conization. Types 6 and 11, at low risk of evolution, are less frequent than 31 and 33, at medium grade of evolution, and than 16 and 18 which are at high risk of evolution.
D'Antona
N
N
Clinica Ostetrica e Ginecologia, Università di Siena.
Tosi
P
P
Ricci
M G
MG
Cintorino
M
M
Bernabei
A
A
Leoncini
P
P
Nuti
S
S
D000328:Adult; D015344:DNA Probes, HPV; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D006801:Humans; D016131:Lymphocyte Subsets; D008875:Middle Aged; D009693:Nucleic Acid Hybridization; D027383:Papillomaviridae / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D014412:Tumor Virus Infections / Q000175:diagnosis*; D002577:Uterine Cervical Diseases / Q000382:microbiology* / Q000473:pathology
D015344:DNA Probes, HPV; D004279:DNA, Viral