title: Localization of preovulatory expression of plasminogen activator inhibitor type-1 and tissue inhibitor of metalloproteinase type-1 mRNAs in the rat ovary.
abstract: Proteinases and their inhibitors control follicular connective tissue remodeling associated with follicular rupture. We examined the regulation and cellular localization of plasminogen activator inhibitor type-1 (PAI-1) and tissue inhibitor of metalloproteinase type-1 (TIMP-1) mRNAs by in situ hybridization. [35S]UTP-labeled RNA probes were hybridized to ovarian sections of eCG-primed immature rats treated with hCG. Before hCG stimulation of ovulation, very low expression of PAI-1 mRNA was observed in theca cells. After hCG administration, expression of PAI-1 mRNA was increased in theca cells of most antral follicles, whereas expression in granulosa cells was limited to preovulatory follicles and only to areas where the basal membrane was dissociated. Before hCG treatment, low expression of TIMP-1 mRNA was observed in theca cells, but not in granulosa cells. After hCG treatment, TIMP-1 mRNA was greatly stimulated in theca cells irrespective of follicle size, while the expression in granulosa cells was limited to large antral follicles. The present study demonstrates cell-specific expression of PAI-1 and TIMP-1 mRNAs in the LH/hCG-stimulated ovary, thus confirming the localized control of preovulatory proteolysis by coexpression of both enzymes and their respective inhibitors.
authors.affiliation: Department of Hormone Research, Bernhard Zondek Hormone Research Laboratory, Weizmann Institute of Science, Rehovot, Israel.
authors.forename: S Y
authors.initials: SY
authors.lastname: Chun
authors.forename: M
authors.initials: M
authors.lastname: Popliker
authors.forename: R
authors.initials: R
authors.lastname: Reich
authors.forename: A
authors.initials: A
authors.lastname: Tsafriri
mesh_terms: D000818:Animals; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D005260:Female; D015870:Gene Expression / Q000187:drug effects / Q000235:genetics; D006023:Glycoproteins / Q000032:analysis* / Q000235:genetics; D006107:Granulosa Cells / Q000737:chemistry / Q000166:cytology; D003012:Microbial Collagenase / Q000037:antagonists & inhibitors; D009693:Nucleic Acid Hybridization; D010053:Ovary / Q000737:chemistry* / Q000166:cytology; D010060:Ovulation / Q000502:physiology; D017395:Plasminogen Activator Inhibitor 1 / Q000032:analysis*; D012333:RNA, Messenger / Q000032:analysis* / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D013799:Theca Cells / Q000737:chemistry / Q000166:cytology; D019714:Tissue Inhibitor of Metalloproteinases
chemical_list: D006063:Chorionic Gonadotropin; D006023:Glycoproteins; D017395:Plasminogen Activator Inhibitor 1; D012333:RNA, Messenger; D019714:Tissue Inhibitor of Metalloproteinases; D003012:Microbial Collagenase