A simple and selective method for visualizing the kinetoplast of trypanosomes by means of phosphomolybdic acid and a photographic developer.
Trypanosoma cruzi epimastigotes treated with phosphomolybdic acid and the photographic developer Rodinal display bright kinetoplasts when unmounted smears are observed using low-magnification phase-contrast objectives. This simple method enables the rapid and accurate identification of kinetoplasts in trypanosomes.
Stockert
J C
JC
Max-Planck-Institut für Biologie, Abteilung Mikrobiologie, Tübingen, Federal Republic of Germany.
D000818:Animals; D004270:DNA, Circular / Q000032:analysis; D018105:DNA, Kinetoplast; D016054:DNA, Protozoan / Q000032:analysis; D007202:Indicators and Reagents; D008982:Molybdenum; D015388:Organelles / Q000648:ultrastructure*; D010756:Phosphoric Acids; D014349:Trypanosoma cruzi / Q000737:chemistry / Q000648:ultrastructure*
D004270:DNA, Circular; D018105:DNA, Kinetoplast; D016054:DNA, Protozoan; D007202:Indicators and Reagents; D010756:Phosphoric Acids; D008982:Molybdenum; C003125:phosphomolybdic acid