title: Immunocytochemical and ultrastructural studies of neuronal and oligodendroglial cytoplasmic inclusions in multiple system atrophy. 2. Oligodendroglial cytoplasmic inclusions.
abstract: Oligodendroglial cytoplasmic inclusions (OCI) in multiple system atrophy were investigated immunocytochemically and ultrastructurally. Among the 17 cases examined, 16 had OCIs. Almost all OCIs were positive for both alpha B-crystallin and ubiquitin. The antibodies against tubulin, paired helical filament and tau stained OCIs to various extents. Ultrastructurally OCIs consisted of meshworks of granule-associated filaments about 25 nm in diameter that sometimes formed flame-shaped tangle-like structures. Immunoelectron microscopy showed that an epitope of alpha B-crystallin was located on the granule-associated filaments composing OCIs. Our studies further support a cooperative role of alpha B-crystallin, ubiquitin and cytoskeletal protein in the formation of some types of intracytoplasmic inclusions.
authors.affiliation: Department of Neuropathology, Faculty of Medicine, University of Tokyo, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Murayama
authors.forename: K
authors.initials: K
authors.lastname: Arima
authors.forename: Y
authors.initials: Y
authors.lastname: Nakazato
authors.forename: J
authors.initials: J
authors.lastname: Satoh
authors.forename: M
authors.initials: M
authors.lastname: Oda
authors.forename: T
authors.initials: T
authors.lastname: Inose
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001284:Atrophy; D002493:Central Nervous System Diseases / Q000378:metabolism / Q000473:pathology*; D002648:Child; D002675:Child, Preschool; D003459:Crystallins / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism / Q000648:ultrastructure*; D006801:Humans; D007150:Immunohistochemistry; D002479:Inclusion Bodies / Q000378:metabolism* / Q000648:ultrastructure; D007223:Infant; D007231:Infant, Newborn; D008854:Microscopy, Electron; D016253:Microscopy, Immunoelectron; D008875:Middle Aged; D009474:Neurons / Q000378:metabolism / Q000648:ultrastructure*; D009836:Oligodendroglia / Q000378:metabolism / Q000648:ultrastructure*; D014404:Tubulin / Q000378:metabolism; D014452:Ubiquitins / Q000378:metabolism; D016875:tau Proteins / Q000378:metabolism
chemical_list: D003459:Crystallins; D014404:Tubulin; D014452:Ubiquitins; D016875:tau Proteins