Macrophage activation by OM-85 BV.
Peritoneal or bone-marrow-derived murine macrophages were exposed for 24 h in vitro to dilutions of the bacterial extract OM-85 BV, in the presence or absence of other added compounds [macrophage-activating factor (MAF), recombinant murine interferon-gamma (IFN-gamma)]. Various metabolic responses and functional activities were then measured. Glucose oxidation through the hexose monophosphate shunt pathway was markedly stimulated in OM-85 BV-treated macrophages compared to control macrophages. Similarly, OM-85 BV primed macrophages for superoxide production upon triggering by phorbol myristate acetate. Both effects were further enhanced by simultaneous treatment of the cells with MAF with OM-85 BV. The bacterial extract also induced macrophages to release large amounts of nitrite (a marker of the activated state). As regards functional responses, coincubation with MAF and OM-85 BV activated macrophages to destroy target cells as well as intracellular microorganisms; in the latter case, similar results were obtained when MAF was replaced by IFN-gamma. In all these tests, the possibility that the observed effects were due to contamination of the bacterial extracts by endotoxin could be excluded. The above results indicate that OM-85 BV induces metabolic and functional properties in macrophages that are characteristic of the activated state and are important for host defence.
Mauël
J
J
Institute of Biochemistry, Epalinges, Switzerland.
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D001419:Bacteria; D002457:Cell Extracts; D005947:Glucose / Q000378:metabolism; D007371:Interferon-gamma / Q000494:pharmacology; D008262:Macrophage Activation; D016215:Macrophage-Activating Factors / Q000494:pharmacology; D008264:Macrophages / Q000276:immunology / Q000378:metabolism; D051379:Mice; D009609:Nitrous Oxide / Q000378:metabolism; D011994:Recombinant Proteins; D013481:Superoxides / Q000378:metabolism; D014407:Tumor Cells, Cultured / Q000276:immunology; D000095983:Bacterial Lysates
D000276:Adjuvants, Immunologic; D002457:Cell Extracts; D005947:Glucose; D007371:Interferon-gamma; D016215:Macrophage-Activating Factors; D009609:Nitrous Oxide; D011994:Recombinant Proteins; D013481:Superoxides; C030259:Broncho-Vaxom; D000095983:Bacterial Lysates