title: [The early diagnosis of leptospirosis by demonstrating antigens via an immunohistochemical exam of the hamstring muscle].
abstract: Establishing an early and certain diagnosis of leptospirosis has been a difficult task. So, we analysed the histopathological alterations of the gastrocnemius muscle, and studied, for the first time, the usefulness of the immune histochemical method of peroxidase-antiperoxidase for the demonstration of the spirochete and of its products in this tissue. Histopathological observations have shown a picture of myositis, characterized by interstitial inflammatory infiltrate and necrotic-degenerative abnormalities of muscle fibers. The lesions were considered minimal in 69.45% of the patients, moderate in 19.45%, severe in 5.55% and absent in the remaining. On the other hand, the immune histochemical method identified the etiology in 94.45% what was considered very expressive.
authors.affiliation: Divisão de Clínica de Moléstias Infecciosas e Parasitárias, Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo.
authors.forename: D E
authors.initials: DE
authors.lastname: Uip
authors.forename: V
authors.initials: V
authors.lastname: Amato Neto
authors.forename: M S
authors.initials: MS
authors.lastname: Duarte
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000942:Antigens, Bacterial / Q000378:metabolism*; D001706:Biopsy; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007921:Leptospira interrogans / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D009132:Muscles / Q000378:metabolism* / Q000473:pathology; D009220:Myositis / Q000175:diagnosis / Q000473:pathology; D013997:Time Factors; D014895:Weil Disease / Q000175:diagnosis* / Q000473:pathology
chemical_list: D000942:Antigens, Bacterial
vernacular_title: Diagnóstico precoce da leptospirose por demonstração de antígenos através de exame imuno-histoquímico em músculo da panturrilha.