[Forskolin and cyclic adenosine monophosphate inhibit the neurulation action of concanavalin A on explants of the gastrula ectoderm of amphibians].
We studied effects of forskolin, an activator of adenylate cyclase activity, and dioctanoyl-cyclic adenosine monophosphate (do-cAMP) on neutralizing (N) activity of concanavalin A (Con A). Biological testing was performed using explanted animal pole ectoderm of the Rana temporaria early gastrula. Con A treatment (200 micrograms/ml, 2 h) resulted in neutralization of 70-90% explants. If the explants were previously treated with forskolin (100 microM, 1 h), Con A effect decreased to 10%. When Con A and forskolin were applied simultaneously, no N-effect was observed. The same results were obtained with simultaneous treatment of the explants with Con A and do-cAMP (10(-5) M). Moreover, treatment with forskolin of the explants previously treated with Con A inhibited their neural differentiation. We suggest that N-effect of Con A is calcium dependent; the increase in intracellular cAMP after treatment of explants with forskolin or do-cAMP interferes with intracellular Ca2+ release and this results in the inhibited N-effect of Con A.
Mikhaĭlov
A T
AT
Gorgoliuk
N A
NA
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D005576:Colforsin / Q000494:pharmacology*; D003208:Concanavalin A / Q000037:antagonists & inhibitors*; D046508:Culture Techniques; D000242:Cyclic AMP / Q000031:analogs & derivatives* / Q000494:pharmacology; D004347:Drug Interactions; D004475:Ectoderm / Q000166:cytology / Q000187:drug effects*; D005775:Gastrula / Q000166:cytology / Q000187:drug effects*; D009420:Nervous System / Q000187:drug effects* / Q000196:embryology; D011896:Rana temporaria; D012996:Solutions
C069614:N(6),O(2)-dioctanoyl cyclic AMP; D012996:Solutions; D003208:Concanavalin A; D005576:Colforsin; C048930:N(1),O(2)-dioctanoyl cyclic AMP; D000242:Cyclic AMP
Forskolin i tsiklicheskiĭ adenozin monofosfat ingibiruiut neĭtralizuiushchee deĭstvie konkanavalina A na ékstrakty éktodermy gastruly amfibiĭ.