title: Antibodies directed against synthetic peptides distinguish between the catalytic subunits of protein phosphatases 1 and 2A.
abstract: Two antipeptide antibodies (designated type 1 antibody and type 2A antibody) were raised against synthetic peptides, Cys-Thr-Pro-Pro-Arg-Asn-Ser-Ala-Lys-Ala-Lys-Lys and Cys-Val-Thr-Arg-Arg-Thr-Pro-Asp-Try-Phe-Leu, corresponding to the carboxyl termini of the catalytic subunits of protein phosphatase 1 and phosphatase 2A (Cys was added for specific coupling to carrier protein). These antipeptide antibodies were highly specific and were useful in discriminating between protein phosphatase 1 and phosphatase 2A in crude extracts or purified preparations. Type 2A antibody reacted with both native and denatured protein phosphatase 2A whereas under the same condition type 1 antibody reacted only with denatured protein phosphatase 1.
authors.affiliation: Department of Pharmacology, Medical College of Ohio, Toledo 43699.
authors.forename: W
authors.initials: W
authors.lastname: Wang
authors.forename: R D
authors.initials: RD
authors.lastname: Lane
authors.forename: K K
authors.initials: KK
authors.lastname: Schlender
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D008969:Molecular Sequence Data; D010455:Peptides / Q000276:immunology; D010749:Phosphoprotein Phosphatases / Q000276:immunology*; D054645:Protein Phosphatase 1; D054648:Protein Phosphatase 2; D011817:Rabbits
chemical_list: D010455:Peptides; D010749:Phosphoprotein Phosphatases; D054645:Protein Phosphatase 1; D054648:Protein Phosphatase 2