High performance liquid chromatography and preliminary pharmacokinetics of rufloxacin and its metabolites N-desmethylrufloxacin and rufloxacinsulfoxide in plasma and urine of humans.
An HPLC analysis was developed for the measurement of rufloxacin and two of its possible metabolites N-desmethylrufloxacin and rufloxacinsulfoxide. Humans are unable to form these two metabolites, while no other metabolites could be detected in the HPLC chromatogram. The half-life of elimination of rufloxacin in two human subjects was 27 and 38 h respectively, while a constant percentage of 25-26% of the dose is excreted unchanged in the urine.
Vree
T B
TB
Department of Clinical Pharmacy, Academic Hospital Nijmegen Sint Radboud, The Netherlands.
van den Biggelaar-Martea
M
M
Imbimbo
B P
BP
D000328:Adult; D000890:Anti-Infective Agents / Q000097:blood / Q000493:pharmacokinetics* / Q000652:urine; D001798:Blood Proteins / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D005260:Female; D024841:Fluoroquinolones; D006801:Humans; D008297:Male; D008875:Middle Aged; D010865:Pilot Projects; D011485:Protein Binding; D015363:Quinolones / Q000032:analysis* / Q000097:blood / Q000493:pharmacokinetics* / Q000652:urine; D013454:Sulfoxides / Q000032:analysis* / Q000097:blood / Q000652:urine; D013843:Thiazines / Q000032:analysis* / Q000097:blood / Q000652:urine
D000890:Anti-Infective Agents; D001798:Blood Proteins; D024841:Fluoroquinolones; D015363:Quinolones; D013454:Sulfoxides; D013843:Thiazines; C074116:N-desmethylrufloxacin; C074117:rufloxacin sulfoxide; C060328:rufloxacin