title: Rapid isoelectric focusing of proteins in hydrolytically stable capillaries.
abstract: Three new types of capillary coatings for capillary isoelectric focusing that avoid siloxane chemistry, resulting in hydrolytically stable coatings, are described and tested: phenyl-silica, acrylamide-reacted vinyl-silica, and pure PTFE. Capillaries of these three types were compared using standard proteins and a biological mixture of proteins similar to what might be encountered in actual use. Of these, the acrylamide-coated capillary produced the highest-quality results. In contrast to capillaries prepared using siloxane reactions, the capillaries described herein exhibited greatly enhanced stability at high pH.
authors.affiliation: Neural Systems Section, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892.
authors.forename: T J
authors.initials: TJ
authors.lastname: Nelson
mesh_terms: D000178:Acrylamides; D000818:Animals; D002417:Cattle; D004586:Electrophoresis; D006454:Hemoglobins / Q000302:isolation & purification; D006868:Hydrolysis; D007525:Isoelectric Focusing / Q000379:methods*; D011138:Polytetrafluoroethylene; D011506:Proteins / Q000302:isolation & purification*; D012710:Serum Albumin, Bovine / Q000302:isolation & purification; D012822:Silicon Dioxide; D012908:Snails / Q000737:chemistry; D014753:Vinyl Compounds
chemical_list: D000178:Acrylamides; D006454:Hemoglobins; D011506:Proteins; D014753:Vinyl Compounds; D012710:Serum Albumin, Bovine; D012822:Silicon Dioxide; D011138:Polytetrafluoroethylene