D-galactosamine-induced cell damage enhances specific binding of prostaglandin E1 to rat hepatocytes.
Specific bindings of [3H]prostaglandin E1 ([3H]PGE1), 125I-glucagon and [3H]norepinephrine to D-galactosamine (GalN)-treated rat hepatocytes in primary culture were investigated. After a two hour-treatment with GalN (1 and 10mg/ml), hepatocytes showed an enhanced specific binding to [3H]PGE1, whereas 125I-glucagon binding was little affected and [3H]norepinephrine binding was strongly diminished. Scatchard plot analysis indicated an increase of binding sites of [3H]PGE1. This unusual manner of [3H]PGE1 binding is suggested to indicate a special property of PGE1 receptor and may be associated with the cytoprotective effect of prostaglandins.
Matsumoto
M
M
Third Department of Internal Medicine, Nara Medical University, Japan.
Kikuchi
E
E
Okamoto
Y
Y
Morimura
M
M
Kitano
H
H
Fukui
H
H
Nakano
H
H
Tsujii
T
T
D000527:Alprostadil / Q000378:metabolism*; D000818:Animals; D001665:Binding Sites; D001667:Binding, Competitive; D002251:Carbon Tetrachloride / Q000494:pharmacology; D002478:Cells, Cultured; D003513:Cycloheximide / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D005688:Galactosamine / Q000494:pharmacology / Q000633:toxicity*; D005934:Glucagon / Q000378:metabolism; D007700:Kinetics; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000494:pharmacology; D008099:Liver / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D009638:Norepinephrine / Q000378:metabolism; D019308:Palmitic Acid; D010169:Palmitic Acids / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D011982:Receptors, Prostaglandin / Q000187:drug effects / Q000378:metabolism*; D018078:Receptors, Prostaglandin E
D008041:Linoleic Acids; D010169:Palmitic Acids; D011982:Receptors, Prostaglandin; D018078:Receptors, Prostaglandin E; D003609:Dactinomycin; D019308:Palmitic Acid; D005688:Galactosamine; D005934:Glucagon; D003513:Cycloheximide; D019787:Linoleic Acid; D002251:Carbon Tetrachloride; D000527:Alprostadil; D009638:Norepinephrine