title: Gene transfer in primary cultures of human hepatocytes.
abstract: Using liposomes as the mediator of DNA transfer, we were successful in the transfection of human hepatocytes isolated from surgical samples with an E. coli beta-galactosidase gene (beta-gal). A comparison of transfection efficiency showed that of the four promoters used, cytomegalovirus (CMV) promoter yielded higher transfection efficiencies than Rous sarcoma virus (RSV), Simian virus-40 (SV-40) and human alpha-1 antitrypsin (AAT) promoters. These studies represent the first report on the successful transfection of primary cultures of human hepatocytes.
authors.affiliation: Health Sciences Department, Monsanto Company, St. Louis, Missouri 63167.
authors.forename: A P
authors.initials: AP
authors.lastname: Li
authors.forename: C A
authors.initials: CA
authors.lastname: Myers
authors.forename: D L
authors.initials: DL
authors.lastname: Kaminski
mesh_terms: D001358:Avian Sarcoma Viruses / Q000235:genetics; D002478:Cells, Cultured; D003587:Cytomegalovirus / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D005814:Genes, Viral; D006801:Humans; D008081:Liposomes; D008099:Liver / Q000201:enzymology*; D011401:Promoter Regions, Genetic; D013539:Simian virus 40 / Q000235:genetics; D014162:Transfection; D000515:alpha 1-Antitrypsin / Q000235:genetics; D001616:beta-Galactosidase / Q000235:genetics*
chemical_list: D008081:Liposomes; D000515:alpha 1-Antitrypsin; D001616:beta-Galactosidase