Immediate cell membrane damage produced by Entamoeba histolytica subcellular extracts.
The alpha[14C]amino isobutyric acid is a synthetic amino acid with low molecular weight, not incorporated to protein. Chinese hamster ovary (CHO) cells were labeled with this radioactive compound and treated with the amebal subcellular fraction P30 at short times. We detected membrane damage in CHO cells by specific radiolabel release from the first 30 sec of interaction between cell cultures and amebal fraction.
Castro-Garza
J E
JE
Departamento de Biología Celular, Unidad de Investigación Biomédica del Noreste, IMSS, Monterrey, NL.
Said-Fernández
S
S
D000818:Animals; D016466:CHO Cells; D002462:Cell Membrane / Q000473:pathology*; D006224:Cricetinae; D003412:Cricetulus; D004748:Entamoeba histolytica / Q000737:chemistry* / Q000472:pathogenicity; D013347:Subcellular Fractions