title: Identification and characterization of glycoprotein gp1 of bovine herpesvirus type 4.
abstract: Three major bovine herpesvirus type 4 (BHV-4) glycoproteins have been described previously. By using monoclonal antibodies produced against BHV-4 envelope proteins from which the three major antigens had been removed by immunoaffinity, a fourth glycoprotein was identified. This protein (gp1) has a high Mr (greater than 300K), is detected about 8 h post-inoculation of infected cells and is strictly expressed as a gamma protein. Moreover, gp1 was identified by a polyclonal antiserum from an infected animal, indicating that this glycoprotein is an antigen recognized by the immune system of infected animals.
authors.affiliation: Department of Virology-Immunology, Faculty of Veterinary Medicine, University of Liège, Belgium.
authors.forename: J
authors.initials: J
authors.lastname: Dubuisson
authors.forename: P P
authors.initials: PP
authors.lastname: Pastoret
authors.forename: E
authors.initials: E
authors.lastname: Thiry
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral / Q000097:blood; D002417:Cattle / Q000382:microbiology*; D002478:Cells, Cultured; D006023:Glycoproteins / Q000096:biosynthesis / Q000276:immunology*; D006564:Herpesviridae / Q000276:immunology*; D006566:Herpesviridae Infections / Q000276:immunology* / Q000662:veterinary; D011233:Precipitin Tests; D014759:Viral Envelope Proteins / Q000096:biosynthesis / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D006023:Glycoproteins; D014759:Viral Envelope Proteins