Neuronal-type nicotinic receptors in human neuroblastoma and small-cell lung carcinoma cell lines.
A beta subunit of the neuronal nicotinic receptor, sharing 88% homology with the rat beta 4 subunit, has been cloned from a human neuroblastoma cell line. The gene encoding the human beta 4 subunit is expressed in association with the alpha 3 gene in neuroblastoma and small-cell lung carcinoma cell lines. Patch-clamp experiments and radioligand binding assays confirm that these neuroendocrine tumor cell lines express functional neuronal nicotinic receptors. We suggest that these receptors might play a crucial role in the control of neurotransmitter and hormone secretion from neurosecretory human tumors.
Tarroni
P
P
Department of Medical Pharmacology, University of Milan, Italy.
Rubboli
F
F
Chini
B
B
Zwart
R
R
Oortgiesen
M
M
Sher
E
E
Clementi
F
F
D000109:Acetylcholine / Q000494:pharmacology; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002217:Carbachol / Q000494:pharmacology; D018288:Carcinoma, Small Cell; D006801:Humans; D008175:Lung Neoplasms; D046911:Macromolecular Substances; D008564:Membrane Potentials / Q000187:drug effects; D008969:Molecular Sequence Data; D009447:Neuroblastoma; D009474:Neurons / Q000502:physiology*; D009838:Oligodeoxyribonucleotides; D009861:Onium Compounds / Q000378:metabolism / Q000494:pharmacology; D016133:Polymerase Chain Reaction / Q000379:methods; D051381:Rats; D011978:Receptors, Nicotinic / Q000187:drug effects / Q000235:genetics* / Q000502:physiology; D017386:Sequence Homology, Amino Acid; D014320:Triphenylmethyl Compounds / Q000378:metabolism / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D046911:Macromolecular Substances; D009838:Oligodeoxyribonucleotides; D009861:Onium Compounds; D011978:Receptors, Nicotinic; D014320:Triphenylmethyl Compounds; C011561:triphenylmethylphosphonium; D002217:Carbachol; D000109:Acetylcholine