Mumps and Coxsackie B3 virus infection of human fetal pancreatic islet-like cell clusters.
Islet-like cell clusters (ICCs) prepared from human fetal pancreases were infected with mumps or coxsackie B3 virus. Double-labeled antibody technique showed that the viruses infected both insulin-secreting and other pancreatic cells and that secretion of immunoreactive insulin into the culture medium of the mumps virus-infected cells had already ceased on day 7. The mumps virus-infected ICC clusters produced virus for 14 days, and the mumps virus antigen was detected in the ICCs through the whole 22-day observation period. The coxsackie B3 virus-infected ICCs contained cells highly positive for viral antigen during the first 2 days after infection, and the infectious virus was detected in the culture medium for 22 days. This in vitro model indicates that mumps and coxsackie B3 viruses infect human fetal pancreatic endocrine cells and are able to alter beta-cell function. Coxsackie B3 virus infection in ICCs is lytical and seems to lead to rapid cell destruction, but long-lasting, restricted mumps virus infection in human fetal pancreatic ICCs offers an interesting model to study the effects of viral infection in the endocrine pancreas and beta cell.
Vuorinen
T
T
Department of Virology, University of Turku, Finland.
Nikolakaros
G
G
Simell
O
O
Hyypiä
T
T
Vainionpää
R
R
D002478:Cells, Cultured; D003384:Coxsackievirus Infections / Q000503:physiopathology*; D029822:Enterovirus B, Human / Q000302:isolation & purification / Q000502:physiology; D005333:Fetus / Q000166:cytology; D005455:Fluorescent Antibody Technique; D006801:Humans; D007328:Insulin / Q000378:metabolism; D007515:Islets of Langerhans / Q000166:cytology* / Q000382:microbiology* / Q000502:physiology; D009107:Mumps / Q000378:metabolism*; D009109:Mumps virus / Q000302:isolation & purification / Q000502:physiology; D010179:Pancreas / Q000196:embryology*; D011863:Radioimmunoassay; D013997:Time Factors; D014779:Virus Replication / Q000502:physiology
D007328:Insulin