Putative apolipoprotein receptor gene (LRP, A2MR) is not rearranged in either myxoid liposarcoma or lipomas with translocations in 12q13-14.
The APR, also known as LRP, gene is highly homologous to the low-density lipoprotein (LDL)-receptor and encodes a cell surface molecule with biochemical properties consistent with function as a lipoprotein receptor. This gene has been mapped to human chromosomal bands 12q13-q14, a region commonly altered in tumors of adipose cells. The proximity of APR to these breakpoints, coupled with its presumed role in lipid metabolism and possible affect on cell proliferation, suggest it as a candidate gene for adipose tissue tumor formation. Pulsed-field gel analysis was used to develop a physical map covering 750 kilobases (kb) surrounding this gene. Examination of myxoid liposarcomas and lipomas bearing the characteristic translocations (12;16)(q13;p11) or (12;variable)(q14;variable), respectively, excluded the breakpoints from within a 750-kb region surrounding the APR gene. These results suggest that APR is not involved directly in the genetic changes that underlie development or progression of these tumors.
Paulien
S
S
Eleanor Roosevelt Institute for Cancer Research, Denver, Colorado 80206.
Sandberg
A A
AA
Herz
J
J
Gemmill
R M
RM
D001053:Apolipoproteins / Q000235:genetics; D002881:Chromosomes, Human, Pair 12; D016521:Electrophoresis, Gel, Pulsed-Field; D006801:Humans; D008067:Lipoma / Q000235:genetics*; D008080:Liposarcoma / Q000235:genetics*; D026503:Low Density Lipoprotein Receptor-Related Protein-1; D011956:Receptors, Cell Surface / Q000235:genetics*; D015183:Restriction Mapping; D014178:Translocation, Genetic / Q000235:genetics*; D014407:Tumor Cells, Cultured
D001053:Apolipoproteins; D026503:Low Density Lipoprotein Receptor-Related Protein-1; D011956:Receptors, Cell Surface