Determination of enalapril and its active metabolite enalaprilat in plasma and urine by gas chromatography/mass spectrometry.
The method for the simultaneous determination of angiotensin-converting enzyme (ACE) inhibitor enalapril and its active metabolite enalaprilat in plasma and urine was developed by gas chromatography/mass spectrometry. Enalapril and enalaprilat in plasma and urine were extracted and cleaned up by using Sep-Pak C18 and silica cartridges. Derivatization was carried out using diazomethane and trifluoroacetic anhydride. Detection by selected ion monitoring was selected to m/z 288 (enalaprilat) and 302 (enalapril). The detection limit of enalapril and enalaprilat was 200 pg/mL in plasma and 2 ng/mL in urine. This method was applied to the pharmacokinetic analysis of enalapril and enalaprilat in body fluids.
Shioya
H
H
Product Development Laboratories, Sankyo Co. Ltd., Tokyo, Japan.
Shimojo
M
M
Kawahara
Y
Y
D004656:Enalapril / Q000097:blood / Q000378:metabolism* / Q000652:urine; D015773:Enalaprilat / Q000097:blood / Q000378:metabolism* / Q000652:urine; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D015203:Reproducibility of Results
D004656:Enalapril; D015773:Enalaprilat