A structurally abnormal erythropoietin receptor gene in a human erythroleukemia cell line.
Restriction endonuclease mapping demonstrates a 3' end deletion of one erythropoietin receptor (EpoR) gene in TF-1 cells, a human erythroleukemia cell line that overexpresses the EpoR and proliferates in response to erythropoietin (Epo). EpoR mRNA transcripts are highly abundant and normal in size. These findings raise interesting questions about the possible role of this EpoR gene abnormality in the pathogenesis of the erythroleukemia from which this cell line was derived. This is the first report of an abnormal human erythropoietin receptor gene.
Ward
J C
JC
Department of Medicine, University of Minnesota Medical School, Minneapolis.
Harris
K W
KW
Penny
L A
LA
Forget
B G
BG
Kitamura
T
T
Winkelmann
J C
JC
D015152:Blotting, Northern; D015139:Blotting, Southern; D002874:Chromosome Mapping; D004262:DNA Restriction Enzymes / Q000494:pharmacology; D004921:Erythropoietin / Q000494:pharmacology; D006801:Humans; D004915:Leukemia, Erythroblastic, Acute / Q000235:genetics* / Q000473:pathology; D009154:Mutation / Q000235:genetics*; D011956:Receptors, Cell Surface / Q000737:chemistry* / Q000187:drug effects / Q000235:genetics*; D017467:Receptors, Erythropoietin; D014158:Transcription, Genetic / Q000187:drug effects; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000473:pathology / Q000648:ultrastructure
D011956:Receptors, Cell Surface; D017467:Receptors, Erythropoietin; D004921:Erythropoietin; D004262:DNA Restriction Enzymes