The mitochondrial genome of the basidiomycete Agrocybe aegerita: molecular cloning, physical mapping and gene location.
The mtDNA of a wild-type strain of Agrocybe aegerita was purified from mitochondria isolated by cellular fractionation. A representative library was constructed in E. coli by molecular cloning at the HindIII restriction site of pBR322. Southern hybridizations between total DNA of the fungal strain and cloned mitochondrial insert probes were used to establish the restriction map of the mtDNA molecule. Its size was assessed at about 80,500 bp. Four structural genes (for Cox 1, Cox 2, Atp 6, and Atp 8) were located on the map by heterologous hybridizations with oligonucleote probes specific for yeast mitochondrial genes. The location of the genes coding for the large and the small RNAs of the mitochondrial ribosome was determined by hybridization with the E. coli rrnB operon. A comparison of A. aegerita mtDNA organization with that of both phylogenetically close and distant fungi is discussed.
Moulinier
T
T
Laboratoire de Génétique Moléculaire et d'Amélioration des Champignons Cultivés, Université de Bordeaux II-INRA, CRA de La Grande Ferrade, Villenave d'Ornon, France.
Barroso
G
G
Labarère
J
J
D000251:Adenosine Triphosphatases / Q000235:genetics; D001483:Base Sequence; D001487:Basidiomycota / Q000201:enzymology / Q000235:genetics*; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004272:DNA, Mitochondrial / Q000235:genetics*; D003576:Electron Transport Complex IV / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D005800:Genes, Fungal / Q000235:genetics*; D016681:Genome, Fungal; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000235:genetics; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D012407:rRNA Operon / Q000235:genetics
D004272:DNA, Mitochondrial; D009838:Oligodeoxyribonucleotides; D003576:Electron Transport Complex IV; D000251:Adenosine Triphosphatases