title: Determination of enalapril and its active metabolite enalaprilat in plasma and urine by gas chromatography/mass spectrometry.
abstract: The method for the simultaneous determination of angiotensin-converting enzyme (ACE) inhibitor enalapril and its active metabolite enalaprilat in plasma and urine was developed by gas chromatography/mass spectrometry. Enalapril and enalaprilat in plasma and urine were extracted and cleaned up by using Sep-Pak C18 and silica cartridges. Derivatization was carried out using diazomethane and trifluoroacetic anhydride. Detection by selected ion monitoring was selected to m/z 288 (enalaprilat) and 302 (enalapril). The detection limit of enalapril and enalaprilat was 200 pg/mL in plasma and 2 ng/mL in urine. This method was applied to the pharmacokinetic analysis of enalapril and enalaprilat in body fluids.
authors.affiliation: Product Development Laboratories, Sankyo Co. Ltd., Tokyo, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Shioya
authors.forename: M
authors.initials: M
authors.lastname: Shimojo
authors.forename: Y
authors.initials: Y
authors.lastname: Kawahara
mesh_terms: D004656:Enalapril / Q000097:blood / Q000378:metabolism* / Q000652:urine; D015773:Enalaprilat / Q000097:blood / Q000378:metabolism* / Q000652:urine; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D015203:Reproducibility of Results
chemical_list: D004656:Enalapril; D015773:Enalaprilat