title: Three-dimensional structure of Na,K-ATPase determined from membrane crystals induced by cobalt-tetrammine-ATP.
abstract: The three-dimensional structure of Na,K-ATPase has been analyzed with electron microscopy and image processing. The enzyme, purified from pig kidney outer medulla, was arranged in a new form of tetragonal two-dimensional membrane crystals after incubation with cobalt-tetrammine-ATP, a stable MgATP complex analogue. Each continuous protein domain, as delineated by negative stain, consists of two alpha beta-protomers related by a dyad axis. The two rod-like regions are connected by a bridge displaced about 20 A away from the center of the structure toward the lipid bilayer. The domain connecting the two promoters is more constricted and closer to the center of the structure in the Co(NH3)4ATP-induced crystals than in the vanadate-induced p21 crystals. These observations suggest that the difference between previously analyzed dimers of two-dimensional p21 crystals induced with vanadate/magnesium and dimers of p4 crystals induced with Co(NH3)4ATP reflects two different conformational states of the enzyme.
authors.affiliation: Department of Cell Biology, University of Aarhus, Denmark.
authors.forename: E
authors.initials: E
authors.lastname: Skriver
authors.forename: U
authors.initials: U
authors.lastname: Kavéus
authors.forename: H
authors.initials: H
authors.lastname: Hebert
authors.forename: A B
authors.initials: AB
authors.lastname: Maunsbach
mesh_terms: D000255:Adenosine Triphosphate / Q000031:analogs & derivatives; D000818:Animals; D003460:Crystallization; D007091:Image Processing, Computer-Assisted; D007679:Kidney Medulla / Q000201:enzymology; D008854:Microscopy, Electron; D008958:Models, Molecular; D015394:Molecular Structure; D009942:Organometallic Compounds; D011487:Protein Conformation; D000254:Sodium-Potassium-Exchanging ATPase / Q000737:chemistry / Q000302:isolation & purification / Q000648:ultrastructure*; D013552:Swine
chemical_list: D009942:Organometallic Compounds; C020511:tetraamminecobalt(III)ATP; D000255:Adenosine Triphosphate; D000254:Sodium-Potassium-Exchanging ATPase