H+/glycyl-glycine cotransport in eel intestinal brush-border membrane vesicles: studies with the pH-sensitive dye Acridine orange.
Monitoring the fluorescence quenching of the pH-sensitive dye Acridine orange, proton accumulation in the presence of an inside-negative transmembrane potential was measured in eel (Anguilla anguilla) intestinal brush-border membrane vesicles. It was demonstrated that the proton accumulation was specifically increased by the presence of the dipeptide glycyl-glycine in the extravesicular space, showing saturation kinetics at increasing dipeptide concentrations and was specifically inhibited by diethylpyrocarbonate. Data reported suggest the presence of an electrical-potential-dependent H+/glycyl-glycine cotransport system in the eel intestinal brush-border membrane vesicles.
Verri
T
T
Dipartimento di Biologia, Università di Lecce, Italy.
Maffia
M
M
Storelli
C
C
D000165:Acridine Orange; D000818:Animals; D004524:Eels; D006033:Glycylglycine / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007413:Intestinal Mucosa / Q000378:metabolism*; D007700:Kinetics; D008871:Microvilli / Q000378:metabolism*; D011522:Protons; D013050:Spectrometry, Fluorescence
D011522:Protons; D006033:Glycylglycine; D000165:Acridine Orange