The human 5-HT2 receptor is encoded by a multiple intron-exon gene.
Serotonin (5-hydroxytryptamine; 5-HT) mediates many central and peripheral nervous system functions by its interaction with specific neuronal receptors. Here we report the genomic structure of the human 5-HT2 receptor. The SacI-EcoRI restriction fragment of rat 5-HT2 receptor cDNA was used as a probe to identify and isolate two positive clones of 8.5 and 7.0 kb from an EcoRI restriction digest of a chromosome 13 specific EcoRI fragment lambda-phage human genomic library. Subcloning and sequencing of these fragments showed the 8.5 kb fragment (designated lambda SE-5) contained the first two exons of the 5-HT2 receptor gene. The 7.0 kb insert (lambda SE-2) contained an incomplete third exon. A HindIII-EcoRI fragment of this insert was used as a probe to isolate a 9.0 kb clone (lambda SH-2), which contained the entire third exon, from a chromosome 13 specific HindIII-fragment lambda-phage human genomic library. The isolation of these three clones (lambda SE-5, lambda SE-2 and lambda SH-2) shows that the human 5-HT2 receptor gene consists of three exons separated by two introns and spans over 20 kb. The deduced amino acid sequence of the human, mouse and rat 5-HT2 receptors are highly conserved and all three share a 90% sequence similarity.
Chen
K
K
Department of Molecular Pharmacology, School of Pharmacy, University of Southern California, Los Angeles 90033.
Yang
W
W
Grimsby
J
J
Shih
J C
JC
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005091:Exons; D019204:GTP-Binding Proteins / Q000502:physiology; D005796:Genes; D006801:Humans; D007438:Introns; D051379:Mice / Q000235:genetics; D008969:Molecular Sequence Data; D051381:Rats / Q000235:genetics; D011985:Receptors, Serotonin / Q000145:classification / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D015398:Signal Transduction; D013045:Species Specificity
D011985:Receptors, Serotonin; D019204:GTP-Binding Proteins