Sequences of DNA fragments contacting the nuclear lamina in vivo.
To study the DNA sequences contacting the nuclear lamina (NL) in vivo, Ehrlich ascites tumor cells were UV-irradiated. The NL was purified, and the DNA fragments covalently linked to the lamina proteins in vivo were cloned and sequenced. Although heterogeneous in length and composition, the sequences displayed homology to the introns and/or flanking regions of different genes, suggesting that functionally distinct regions are organized in a topologically defined manner at the nuclear periphery.
Christova
R
R
Institute of Molecular Biology, Bulgarian Academy of Sciences, Sofia.
Bach
I
I
Galcheva-Gargova
Z
Z
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000235:genetics; D002843:Chromatin / Q000378:metabolism; D003001:Cloning, Molecular; D003432:Cross-Linking Reagents; D004273:DNA, Neoplasm / Q000235:genetics* / Q000528:radiation effects; D003852:Deoxyribonucleoproteins / Q000235:genetics*; D007438:Introns; D034882:Lamins; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000235:genetics / Q000528:radiation effects; D015530:Nuclear Matrix / Q000378:metabolism*; D009687:Nuclear Proteins / Q000235:genetics* / Q000528:radiation effects; D012091:Repetitive Sequences, Nucleic Acid; D012689:Sequence Homology, Nucleic Acid; D014407:Tumor Cells, Cultured; D014466:Ultraviolet Rays
D002843:Chromatin; D003432:Cross-Linking Reagents; D004273:DNA, Neoplasm; D003852:Deoxyribonucleoproteins; D034882:Lamins; D009363:Neoplasm Proteins; D009687:Nuclear Proteins