[Changes in the gene expression of the hepatitis B virus in PLC-PRF-5 cells of human hepatocarcinoma during their acquisition of drug resistance].
Mutant clones of hepatocarcinoma cell line PLC-PRF-5 containing integrated hepatitis B virus genome were derived by multistep selection for resistance to some toxic agents. These clones were found to display various stages of cell differentiation according to growth rate, ability to synthesize alpha-fetoprotein, to grow in semisolid medium and to cloning in agar. In the majority of the clones with higher stages of differentiation the expression of HBsAg gene was demonstrated to be increased as compared to the original line, and in some of these clones the expression of silent HBcAg was activated.
Tugizov
Sh M
ShM
Savchenkova
I P
IP
Grabovskaia
I L
IL
Kushch
A A
AA
D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000276:immunology; D002471:Cell Transformation, Neoplastic / Q000235:genetics / Q000276:immunology; D004351:Drug Resistance / Q000235:genetics / Q000276:immunology; D015967:Gene Expression Regulation, Viral / Q000235:genetics* / Q000276:immunology; D016679:Genome, Viral; D006512:Hepatitis B Core Antigens / Q000235:genetics / Q000276:immunology; D006514:Hepatitis B Surface Antigens / Q000235:genetics / Q000276:immunology; D006515:Hepatitis B virus / Q000235:genetics* / Q000276:immunology; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics* / Q000276:immunology; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D012641:Selection, Genetic; D014407:Tumor Cells, Cultured / Q000276:immunology
D006512:Hepatitis B Core Antigens; D006514:Hepatitis B Surface Antigens
Izmenenie ékspressii genov virusa gepatita B v kletkakh gepatokartsinomy cheloveka PLC-PRF-5 v protsesse priobreteniia imi lekarstvennoĭ ustoĭchivosti.