An Epstein-Barr virus transformation-associated membrane protein interacts with src family tyrosine kinases.
In latently infected growth-transformed human lymphocytes, Epstein-Barr virus (EBV) encodes two integral plasma membrane proteins: LMP1, which constitutively induces B-lymphocyte activation and intercellular adhesion, and LMP2A, which associates with LMP1 and is a tyrosine kinase substrate. We now demonstrate that LMP2A associates with src family protein tyrosine kinases, particularly lyn kinase, in nonionic detergent extracts of transfected B lymphoma cells or in extracts of EBV-transformed B lymphocytes. The LMP2A and tyrosine kinase association is stable in nonionic detergents and includes a 70-kDa cell protein which is also an in vitro or in vivo kinase substrate. This LMP2A association with B-lymphocyte src family tyrosine kinases is likely to be an important pathway in EBV's effects on cell growth.
Burkhardt
A L
AL
Department of Molecular Biology, Bristol-Myers Squibb Pharmaceutical Research Institute, Princeton, New Jersey 08543.
Bolen
J B
JB
Kieff
E
E
Longnecker
R
R
D000818:Animals; D000956:Antigens, Viral / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D001402:B-Lymphocytes; D002460:Cell Line; D002472:Cell Transformation, Viral; D016391:Genes, src; D004854:Herpesvirus 4, Human / Q000378:metabolism*; D016393:Lymphoma, B-Cell; D008565:Membrane Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D015688:Oncogene Protein pp60(v-src) / Q000302:isolation & purification / Q000378:metabolism*; D011485:Protein Binding; D011505:Protein-Tyrosine Kinases / Q000302:isolation & purification / Q000378:metabolism*; D014162:Transfection; D014763:Viral Matrix Proteins; D014764:Viral Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*
D000956:Antigens, Viral; C037662:EBV-associated membrane antigen, Epstein-Barr virus; D008565:Membrane Proteins; D014763:Viral Matrix Proteins; D014764:Viral Proteins; D011505:Protein-Tyrosine Kinases; D015688:Oncogene Protein pp60(v-src)