Immobilization of nucleoside on silica gel and specific separation of oligonucleotides.
Deoxyadenosine was immobilized on silica gel, in order to use as HPLC resins for selective separation of oligonucleotides. The longest retention time was observed for the complementary pd(T)4, and increased with decrease of temperature. This fact suggested that the main separation factor was the base pairing between complementary nucleic acid bases. These resins may be useful for separation of components of nucleic acids and polynucleotides as a specific separation system.
Inaki
Y
Y
Faculty of Engineering, Osaka University, Japan.
Matsukawa
K
K
Kawano
H
H
Hong
W
W
Wada
T
T
Mochizuki
E
E
Takemoto
K
K
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003839:Deoxyadenosines / Q000737:chemistry*; D009841:Oligonucleotides / Q000302:isolation & purification*; D058428:Silica Gel; D012822:Silicon Dioxide / Q000737:chemistry*; D013816:Thermodynamics
D003839:Deoxyadenosines; D009841:Oligonucleotides; D058428:Silica Gel; D012822:Silicon Dioxide