Characterization of gold in urine and bile following administration of gold sodium thiomalate with chelating agents to rats.
Gold was characterized in the urine and bile of rats treated with D-penicillamine (D-PEN), 2,3-dimercaptosuccinic acid (DMSA), 2,3-dimercaptopropane sulphonate (DMPS), or N-(2-mercapto-2-methylpropanoyl)-L-cysteine (bucillamine) immediately after gold sodium thiomalate (AuTM) injection by both gel chromatographic and electrophoretic methods. It is suggested that the gold in the urine and bile after AuTM administration was predominantly bound to high molecular weight compounds. The characterization of gold in the urine after administration of AuTM with D-PEN, DMSA, or DMPS showed that most of the gold was bound to the chelating agents. In the treatment with the chelating agents such as D-PEN and DMPS, the gold was mainly excreted as a gold-chelating agent compound in the bile and a minor portion of the gold was present in the form of a gold-L-cysteine compound and high molecular weight compounds. DMSA treatment showed that a major portion of the gold was bound to high molecular weight compounds in the bile and a minor portion of the gold was present in the forms of gold-DMSA and gold-L-cysteine compounds. The administration of AuTM and bucillamine indicated that the gold was mainly present as a gold-Me-bucillamine compound in the urine and a gold-bucillamine compound in the bile.
Kojima
S
S
Department of Hygienic Chemistry, Faculty of Pharmaceutical Sciences, Kumamoto University, Japan.
Takahashi
Y
Y
Kiyozumi
M
M
Funakoshi
T
T
Shimada
H
H
D000818:Animals; D001646:Bile / Q000737:chemistry*; D002614:Chelating Agents / Q000494:pharmacology*; D002845:Chromatography; D003545:Cysteine / Q000031:analogs & derivatives / Q000494:pharmacology; D004586:Electrophoresis; D006046:Gold / Q000032:analysis* / Q000737:chemistry* / Q000652:urine; D006052:Gold Sodium Thiomalate / Q000008:administration & dosage* / Q000378:metabolism; D007275:Injections, Intravenous; D008297:Male; D010396:Penicillamine / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D004113:Succimer / Q000494:pharmacology; D014494:Unithiol / Q000494:pharmacology
D002614:Chelating Agents; D006052:Gold Sodium Thiomalate; D014494:Unithiol; D006046:Gold; D004113:Succimer; D010396:Penicillamine; D003545:Cysteine; C026535:bucillamine