title: Cloning of complementary DNA inserts from phage DNA directly into plasmid vector.
authors.affiliation: Department of Internal Medicine, Ohio State University, Columbus 43210.
authors.forename: I M
authors.initials: IM
authors.lastname: Chiu
authors.forename: K
authors.initials: K
authors.lastname: Lehtoma
authors.forename: M L
authors.initials: ML
authors.lastname: Poulin
mesh_terms: D010582:Bacteriophage lambda / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods*; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004274:DNA, Recombinant / Q000096:biosynthesis*; D004279:DNA, Viral / Q000235:genetics* / Q000302:isolation & purification; D015246:Deoxyribonuclease EcoRI; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D005822:Genetic Vectors; D007202:Indicators and Reagents; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D010957:Plasmids; D016133:Polymerase Chain Reaction / Q000379:methods*; D015183:Restriction Mapping
chemical_list: D004274:DNA, Recombinant; D004279:DNA, Viral; D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides; D004262:DNA Restriction Enzymes; D015246:Deoxyribonuclease EcoRI