[Influence of phosphorylation on the functional properties of the sodium channel reconstructed in an artificial membrane].
Phosphorylation of the reconstructed TTX-sensitive cytosolic protein of the bovine brain has been studied. Some properties of the protein are similar to those of the membrane potential-dependent sodium channel. It is shown that the influence of phosphorylation by protein kinase A on the reconstructed channel greatly depends on the mode of reconstruction. Phosphorylation fo reconstructed channels in the open state leads to their closing. Preliminary phosphorylation of channel-forming protein results in a considerable increase of the activation effect of veratrine and scorpion toxin.
Malysheva
M K
MK
Zhukareva
V A
VA
Kastrikina
T F
TF
D000818:Animals; D002417:Cattle; D008564:Membrane Potentials / Q000502:physiology; D008567:Membranes, Artificial; D009419:Nerve Tissue Proteins / Q000378:metabolism; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism*; D012604:Scorpion Venoms / Q000494:pharmacology; D015222:Sodium Channels / Q000187:drug effects / Q000502:physiology*; D013779:Tetrodotoxin / Q000494:pharmacology; D014702:Veratrine / Q000494:pharmacology
D008567:Membranes, Artificial; D009419:Nerve Tissue Proteins; D012604:Scorpion Venoms; D015222:Sodium Channels; D013779:Tetrodotoxin; D011494:Protein Kinases; D014702:Veratrine
Vliianie fosforilirovaniia na funktsional'nye svoĭstva natrievogo kanala, rekonstruirovannogo v iskusstvennuiu membranu.