Human papillomavirus and herpes virus DNA are not detected in benign and malignant prostatic tissue using the polymerase chain reaction.
Fresh prostatic tissue removed at the time of surgery was assayed for the presence of human papillomavirus (HPV) types 6, 11, 16, 18 and 33 and herpes and varicella-zoster viruses (HV) using DNA amplification followed by specific hybridization. Thirty samples representing both benign and malignant prostatic disease were assayed. Although appropriate amplimers were present for beta globulin gene indicating successful extraction of DNA, no HPV or HV amplimers could be obtained with appropriate primers. We conclude that HPV and HV are not routinely found in human prostate.
Serfling
U
U
Veterans Affairs Medical Center, Department of Dermatology, Miami, Florida.
Ciancio
G
G
Zhu
W Y
WY
Leonardi
C
C
Penneys
N S
NS
D015344:DNA Probes, HPV / Q000032:analysis*; D004279:DNA, Viral / Q000032:analysis*; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D011467:Prostate / Q000737:chemistry*; D011469:Prostatic Diseases / Q000382:microbiology*; D018139:Simplexvirus / Q000235:genetics*
D015344:DNA Probes, HPV; D004279:DNA, Viral