Multiple sequence elements are involved in RNA 3' end formation in spleen necrosis virus.
The function of the poly(A) signal in spleen necrosis virus (SNV) is dependent upon the distance between the cap site and the poly(A) site, while the function of the SV40 late poly(A) signal is independent of the distance. Deletions in the SNV poly(A) sequence do not alter the distance-dependent function. SNV/SV40 chimeric poly(A) signals show intermediate behavior between the SNV and SV40 poly(A) signals. These results indicate that multiple sequence elements are involved in the functions of either the SNV or SV40 poly(A) signals. This intermediate behavior is also observed with poly(A) signals from the mouse alpha-globin and herpes simplex virus thymidine kinase genes.
Iwasaki
K
K
McArdle Laboratory for Cancer Research, University of Wisconsin, Madison 53706.
Temin
H M
HM
D001483:Base Sequence; D002872:Chromosome Deletion; D004252:DNA Mutational Analysis; D008969:Molecular Sequence Data; D011061:Poly A / Q000378:metabolism*; D012323:RNA Processing, Post-Transcriptional / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics / Q000378:metabolism*; D012045:Regulatory Sequences, Nucleic Acid; D012190:Retroviridae / Q000235:genetics*; D013539:Simian virus 40 / Q000235:genetics
D012367:RNA, Viral; D011061:Poly A