Induction of NGF by isoproterenol, 4-methylcatechol and serum occurs by three distinct mechanisms.
Evidence is provided that isoproterenol, 4-methylcatechol and serum induce NGF by three separate mechanisms. Isoproterenol and 4-methylcatechol induced NGF and NGF mRNA in mouse fibroblast L929 cells in either the presence or absence of serum. Propranolol prevented NGF induction by isoproterenol, but not by 4-methylcatechol or serum. All possible combinations of these inducers resulted in additive increases in the levels of NGF and NGF mRNA.
Carswell
S
S
Cephalon, Inc., West Chester, PA 19380.
Hoffman
E K
EK
Clopton-Hartpence
K
K
Wilcox
H M
HM
Lewis
M E
ME
D000818:Animals; D002396:Catechols / Q000494:pharmacology*; D002478:Cells, Cultured; D006736:Horses / Q000276:immunology; D007106:Immune Sera / Q000494:pharmacology*; D007545:Isoproterenol / Q000494:pharmacology*; D007739:L Cells / Q000378:metabolism; D051379:Mice; D009414:Nerve Growth Factors / Q000096:biosynthesis*; D011433:Propranolol / Q000494:pharmacology; D012333:RNA, Messenger / Q000096:biosynthesis
D002396:Catechols; D007106:Immune Sera; D009414:Nerve Growth Factors; D012333:RNA, Messenger; C018599:4-methylcatechol; D011433:Propranolol; D007545:Isoproterenol