Lack of JC viral genomic sequences in multiple sclerosis brain tissue by polymerase chain reaction.
With DNA extracted from brain specimens from 19 multiple sclerosis, 5 progressive multifocal leukoencephalopathy, 1 Alzheimer's disease, and 8 nonneurological control subjects, polymerase chain reaction was performed using nested sets of primer pairs amplifying segments of the large T and VP1 antigen-encoding sequences of JC virus. Both sequences were detected in each of the 5 brain specimens of progressive multifocal leukoencephalopathy but in none of the 19 multiple sclerosis, 1 Alzheimer's disease, or the 8 control brain specimens.
Buckle
G J
GJ
Laboratory of Central Nervous System Studies, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892.
Godec
M S
MS
Rubi
J U
JU
Tornatore
C
C
Major
E O
EO
Gibbs
C J
CJ
Gajdusek
D C
DC
Asher
D M
DM
D001483:Base Sequence; D001921:Brain / Q000382:microbiology; D006801:Humans; D007577:JC Virus / Q000235:genetics / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D009103:Multiple Sclerosis / Q000382:microbiology*; D016133:Polymerase Chain Reaction