title: Identity of amino acid sequences of superoxide dismutase purified from both anaerobically maintained and aerated Porphyromonas gingivalis.
abstract: Superoxide dismutase (SOD) from anaerobically maintained Porphyromonas gingivalis (anaero-SOD) has the characteristic of Fe-SOD, whereas SOD from aerated cells (aero-SOD) has that of Mn-SOD. However, both types of apoSOD can bind either Fe or Mn. To elucidate the structure relationship between anaero- and aero-SOD, we examined the amino acid sequence of aero-SOD by direct protein analysis. The amino acid sequence of aero-SOD was shown to be identical with that of anaero-SOD determined previously. Our findings support the hypothesis that cambialistic SOD is formed from a single apoprotein in bacterial cells.
authors.affiliation: Department of Preventive Dentistry, Faculty of Dentistry, Osaka, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Amano
authors.forename: S
authors.initials: S
authors.lastname: Shizukuishi
authors.forename: A
authors.initials: A
authors.lastname: Tsunemitsu
authors.forename: S
authors.initials: S
authors.lastname: Tsunasawa
mesh_terms: D000332:Aerobiosis; D000595:Amino Acid Sequence; D000693:Anaerobiosis; D003470:Culture Media; D008969:Molecular Sequence Data; D016966:Porphyromonas gingivalis / Q000201:enzymology*; D013482:Superoxide Dismutase / Q000737:chemistry*
chemical_list: D003470:Culture Media; D013482:Superoxide Dismutase