title: Characterization of experimental rat nephroblastoma and its cell line.
abstract: Rat nephroblastoma (Wilms' tumor) was induced by transplacental administration of N-ethyl-nitrosourea (ENU). The induced renal tumors were histologically compatible with human nephroblastoma. A cultured cell line (ENU-T-1) established from a xenotransplant, showed similar morphological and biological features to cultured embryonal kidney cells. Introduction of normal human chromosome #11 (#11) bearing Wilms' tumor suppressor gene(s) (WT) suppressed colony-forming ability on soft agar plates (CFA) but tumorigenicity of ENU-T-1 was not affected. Whereas tumorigenicity of human nephroblastoma cell line, SK-NEP-1 was completely suppressed, CFA was unchanged. These facts indicated that pathogenetic mechanism is different between human and experimental rat nephroblastomas.
authors.affiliation: Department of Pathology, Yokohama City University School of Medicine, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Nagashima
authors.forename: Y
authors.initials: Y
authors.lastname: Miyagi
authors.forename: K
authors.initials: K
authors.lastname: Sumino
authors.forename: Y
authors.initials: Y
authors.lastname: Ohaki
authors.forename: M
authors.initials: M
authors.lastname: Umeda
authors.forename: M
authors.initials: M
authors.lastname: Oshimura
authors.forename: K
authors.initials: K
authors.lastname: Misugi
mesh_terms: D000818:Animals; D005038:Ethylnitrosourea; D006801:Humans; D007680:Kidney Neoplasms / Q000139:chemically induced / Q000473:pathology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014407:Tumor Cells, Cultured; D009396:Wilms Tumor / Q000139:chemically induced / Q000473:pathology*
chemical_list: D005038:Ethylnitrosourea