Changes in surface relief of suspended cells are morphological signs of the initial stage of neoplastic transformation in fibroblastic monolayer cultures.
The percentages of cells with different types of cell surface relief were determined in cell suspensions derived from monolayer cultures. Primary cultures of rat embryo fibroblasts (REF) and cell lines REF (LT) and REF-1, immortalized cells of which preserved normal phenotypic characteristics of the initial primary culture REF, as well as morphologically transformed tumorigenic lines REF (LT) ras and REF-2EJ were studied. In REF suspensions the cells with the blebbed type of surface relief were shown to be predominant as compared with those with microvillus relief whereas cell suspensions derived from both immortalized and fully transformed cultures display the reverse ratio of cells with those types of surface relief. Therefore, the pattern of cell surface relief in cell suspensions derived from fibroblastic monolayer cultures may serve as a morphological marker of the initial stage of neoplastic transformation-immortalization when typical morphological signs of cell transformation are not yet manifested in monolayer cultures.
Rovensky
Y A
YA
Laboratory of Mechanisms of Carcinogenesis, Russian Academy of Medical Sciences, Moscow.
Komissarova
E V
EV
Topol
L Z
LZ
Kisseljov
F L
FL
D000256:Adenoviridae / Q000235:genetics; D017500:Adenovirus E1A Proteins / Q000235:genetics / Q000502:physiology; D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000235:genetics / Q000502:physiology; D016923:Cell Death; D002471:Cell Transformation, Neoplastic / Q000473:pathology*; D002472:Cell Transformation, Viral; D005347:Fibroblasts / Q000473:pathology*; D051379:Mice; D008819:Mice, Nude; D008855:Microscopy, Electron, Scanning; D015513:Oncogene Proteins / Q000235:genetics / Q000502:physiology; D011120:Polyomavirus / Q000235:genetics; D016283:Proto-Oncogene Proteins p21(ras) / Q000235:genetics / Q000502:physiology; D051381:Rats; D011916:Rats, Inbred F344 / Q000196:embryology; D014162:Transfection; D014407:Tumor Cells, Cultured / Q000648:ultrastructure
D017500:Adenovirus E1A Proteins; D000952:Antigens, Polyomavirus Transforming; D015513:Oncogene Proteins; C077183:human oncogene protein EJ-ras; D016283:Proto-Oncogene Proteins p21(ras)