title: Identification of the glycoprotein H gene of murine cytomegalovirus.
abstract: Partial sequencing of the HindIII C fragment of murine cytomegalovirus (MCMV) revealed an open reading frame of 2172 nucleotides in length encoding a 724 amino acid protein with a predicted Mr of 80.4K. Analysis of the predicted amino acid sequence revealed homology with glycoprotein H (gH) from a number of other herpesviruses. MCMV gH showed strongest amino acid identity with human (H) CMV and human herpesvirus 6 gH, and less identity with the gH protein sequences of Epstein-Barr virus, varicella-zoster virus and herpes simplex virus type 1. The greatest identity between MCMV and HCMV gH occurs in the C-terminal region. The MCMV gH is characterized by having a 14 amino acid signal sequence, a 23 amino acid transmembrane region, a seven amino acid positively charged cytoplasmic anchor sequence and eight putative N-linked glycosylation sites. Comparison of MCMV gH with that of HCMV indicates that there are 12 conserved cysteine residues and three conserved potential N-linked glycosylation sites.
authors.affiliation: Department of Microbiology, University of Western Australia, Queen Elizabeth II Medical Centre, Nedlands.
authors.forename: J
authors.initials: J
authors.lastname: Xu
authors.forename: P B
authors.initials: PB
authors.lastname: Dallas
authors.forename: P A
authors.initials: PA
authors.lastname: Lyons
authors.forename: G R
authors.initials: GR
authors.lastname: Shellam
authors.forename: A A
authors.initials: AA
authors.lastname: Scalzo
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003587:Cytomegalovirus / Q000235:genetics*; D005814:Genes, Viral; D006023:Glycoproteins / Q000737:chemistry / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000235:genetics*
chemical_list: D006023:Glycoproteins; D014759:Viral Envelope Proteins