title: Direct effects of bromocriptine on the steroidogenic capability of porcine granulosa cells.
abstract: The direct effects of bromocriptine on steroidogenesis were examined in cultured porcine granulosa cells. The following observations were made with bromocriptine: (1) It significantly increased the basal or FSH-stimulated secretion of progesterone in cultured porcine granulosa cells at concentrations exceeding 10(-7) mol/l; (2) its inhibitory effect on basal estradiol secretion was demonstrated; (3) it did not influence cell number in cultured porcine granulosa cells; (4) it increased the extracellular accumulation of cAMP in a concentration-dependent manner; and (5) it did not induce a change in cytosolic free Ca2+ concentration. These findings suggest that bromocriptine exerts a direct effect on steroidogenesis in ovarian granulosa cells.
authors.affiliation: Department of Obstetrics and Gynecology, Tokyo Medical and Dental University, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Kamada
authors.forename: T
authors.initials: T
authors.lastname: Kubota
authors.forename: M
authors.initials: M
authors.lastname: Taguchi
authors.forename: T
authors.initials: T
authors.lastname: Aso
mesh_terms: D000818:Animals; D001971:Bromocriptine / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004958:Estradiol / Q000378:metabolism; D005260:Female; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D006107:Granulosa Cells / Q000166:cytology / Q000378:metabolism*; D009994:Osmolar Concentration; D011374:Progesterone / Q000378:metabolism; D013256:Steroids / Q000096:biosynthesis*; D013552:Swine
chemical_list: D013256:Steroids; D001971:Bromocriptine; D011374:Progesterone; D004958:Estradiol; D005640:Follicle Stimulating Hormone; D000242:Cyclic AMP; D002118:Calcium