Human papillomavirus detection in cervical cells by in situ hybridization with biotinylated probes.
We have investigated the applicability of human papillomavirus (HPV) DNA detection by in situ hybridization with biotinylated probes in epithelial cells obtained from the cervix using a cotton tip swab. We describe a simple procedure for obtaining homogeneous cell samples and good preservation of cellular structure. This is achieved by pretreatment of cells with L-cysteine before hybridization. Separate denaturation of cellular DNA and probe DNA is also necessary for satisfactory results. Both benign HPV DNA 6/11 and potentially oncogenic HPV DNA 16/18 could be identified in our series. In situ hybridization on cervical scrapes is a rapid, simple and very specific method for detecting patients infected with oncogenic HPV types.
Chardonnet
Y
Y
INSERM U346, Affiliée CNRS, Pav. R, Hôpital E. Herriot, Lyon, France.
Béjui-Thivolet
F
F
Guérin-Reverchon
I
I
D000328:Adult; D001710:Biotin; D015139:Blotting, Southern; D002460:Cell Line; D002584:Cervix Uteri / Q000166:cytology / Q000382:microbiology*; D015344:DNA Probes, HPV; D005260:Female; D006367:HeLa Cells; D006801:Humans; D017403:In Situ Hybridization; D008875:Middle Aged; D027383:Papillomaviridae / Q000302:isolation & purification*; D014626:Vaginal Smears / Q000379:methods*
D015344:DNA Probes, HPV; D001710:Biotin