title: Protein phosphorylation is involved in the regulation of chromatin condensation during interphase.
abstract: Loci affecting the condensation state of interphase chromatin have been previously identified from analysis of suppression and enhancement of position effect variegation (PEV) in Drosophila. Here we show that Su-var(3)6 and an allelic mutant, e078, which both show suppression of PEV in the heterozygous state, have point mutations (Gly220-->Ser and Gly220-->Asp, respectively) in a protein phosphatase 1 catalytic subunit located at 87B (PP1 87B). The mutated glycine is conserved in all known protein serine/threonine phosphatases in the same gene family, and its substitution decreases PP1 activity. We conclude that protein dephosphorylation by PP1 87B regulates the condensation state of chromatin during interphase.
authors.affiliation: Department of Biochemistry, The University, Dundee, Scotland, UK.
authors.forename: V
authors.initials: V
authors.lastname: Dombrádi
authors.forename: P T
authors.initials: PT
authors.lastname: Cohen
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002843:Chromatin / Q000378:metabolism*; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D015342:DNA Probes; D004330:Drosophila / Q000235:genetics / Q000502:physiology*; D007399:Interphase; D007438:Introns; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D005810:Multigene Family; D009154:Mutation; D010749:Phosphoprotein Phosphatases / Q000235:genetics*; D010766:Phosphorylation; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D054645:Protein Phosphatase 1; D017384:Sequence Deletion; D017386:Sequence Homology, Amino Acid
chemical_list: D002843:Chromatin; D015342:DNA Probes; D046911:Macromolecular Substances; D004247:DNA; D010749:Phosphoprotein Phosphatases; D054645:Protein Phosphatase 1