Simple in vitro methods for titrating feline immunodeficiency virus (FIV) and FIV neutralizing antibodies.
The feline immunodeficiency virus (FIV) readily produced syncytia in Crandell feline kidney (CrFK) cells adapted to a medium containing 0.5% fetal calf serum, a variety of growth factors and other supplements. This finding has been exploited to develop simple and sensitive virus titration and neutralization assays. High titre neutralizing antibodies were detected in cats infected naturally and experimentally with FIV, but not in uninfected animals.
Tozzini
F
F
Department of Animal Pathology, University of Pisa, Italy.
Matteucci
D
D
Bandecchi
P
P
Baldinotti
F
F
Poli
A
A
Pistello
M
M
Siebelink
K H
KH
Ceccherini-Nelli
L
L
Bendinelli
M
M
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D015153:Blotting, Western; D002415:Cats; D002460:Cell Line; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D016181:Feline Acquired Immunodeficiency Syndrome / Q000175:diagnosis; D015726:Giant Cells / Q000382:microbiology*; D016089:Immunodeficiency Virus, Feline / Q000276:immunology* / Q000302:isolation & purification* / Q000502:physiology; D007668:Kidney / Q000166:cytology; D009500:Neutralization Tests; D012680:Sensitivity and Specificity
D000914:Antibodies, Viral