18-hydroxylation in the Y-1 adrenal cell line: response to ACTH and to culture conditions.
The 18-hydroxylation of deoxycorticosterone in the Y-1 adrenal cell line was studied under various incubation and cell culture conditions and compared to 11 beta-hydroxylation. Repeated incubation of the substrate increased both 18- and 11 beta-hydroxylation in the Y-1 cells. Furthermore, both 18- and 11 beta-hydroxylation were increased with increased serum concentration and prolonged incubation time. While the increase in 11 beta-hydroxylation seemed to be independent of the type of serum, 18-hydroxylation was much more important in cells cultured in fetal or newborn calf serum supplemented medium than in those cultured in horse serum supplemented medium. As expected, ACTH treatment increased 11 beta-hydroxylation; however, it decreased 18-hydroxylation. The different regulation of these two hydroxylating pathways by ACTH, point to a heterogeneity of the cytochrome P-450(11) beta of the Y-1 cell line.
Es-souni
M
M
Laboratoire de Biochimie, Faculté des Sciences, Université de Bourgogne, Dijon, France.
Ramirez
L C
LC
Bournot
P
P
D000311:Adrenal Glands / Q000187:drug effects / Q000378:metabolism*; D000324:Adrenocorticotropic Hormone / Q000494:pharmacology*; D000818:Animals; D001769:Blood; D002478:Cells, Cultured; D003470:Culture Media / Q000494:pharmacology*; D019405:Cytochrome P-450 CYP11B2; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis*; D003900:Desoxycorticosterone / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D005312:Fetal Blood; D006900:Hydroxylation / Q000187:drug effects; D051379:Mice; D013252:Steroid 11-beta-Hydroxylase / Q000096:biosynthesis; D013250:Steroid Hydroxylases / Q000096:biosynthesis*
D003470:Culture Media; D003900:Desoxycorticosterone; D000324:Adrenocorticotropic Hormone; D003577:Cytochrome P-450 Enzyme System; D013250:Steroid Hydroxylases; D019405:Cytochrome P-450 CYP11B2; D013252:Steroid 11-beta-Hydroxylase