[Plasmid localization and cloning of restriction modification genes from Citrobacter freundii 4111 strain].
Over 60 producing strains of restriction endonucleases type II have been found among 500 different strains, mostly Enterobacteriaceae. The strain Citrobacter freundii 4111 produces restriction endonuclease CfrBI, a new isoschisomer of StyI. The genes of the restriction-modification system CfrBI were located on the multicopy plasmid pZE8 containing the Co1E1-type replicon and cloned to E. coli K802. The deletion variant of 3.2-kb pZE8 which contains intact restriction-modification and a DNA fragment responsible for autonomous plasmid replication was selected among the recombinant plasmids. The strain with higher R. CfrBI production (at least 10,000,000 U/g cells, which is 500-fold higher than the wild strain) was constructed.
Kravets
A N
AN
Solonin
A S
AS
Zakharova
M V
MV
Tarutina
Z E
ZE
D016971:Citrobacter freundii / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes / Q000096:biosynthesis / Q000235:genetics*; D005798:Genes, Bacterial; D010957:Plasmids; D011995:Recombination, Genetic; D013045:Species Specificity
D004262:DNA Restriction Enzymes
Plazmidnaia lokalizatsiia i klonirovanie genov sistemy restriktsii-modifikatsii iz shtamma Citrobacter freundii 4111.