Superoxide production by liver macrophages in a septic rat model--relation to arterial ketone body ratio.
The relationship between superoxide production by liver macrophages and arterial ketone body ratio (AKBR), which reflects the oxidation-reduction state in the mitochondrial compartment of hepatocytes, was studied in rats with lethal and sublethal septicemia induced by intravenous injection of live Escherichia coli 014. In the sublethal model, AKBR decreased transiently (p < 0.01) and superoxide production by isolated liver macrophages increased significantly after opsonized zymosan (OZ) stimulation (p < 0.05). On the other hand, in the lethal model, AKBR decreased markedly (p < 0.01) to below 0.4 without recovery, and superoxide production was not activated by OZ stimulation. Thus, when AKBR decreases to an irreversible level, below about 0.4, superoxide production by liver macrophages is impaired, while as long as AKBR remains reversible, more than about 0.4, it is enhanced. It is suggested that superoxide production by the Kupffer cells is related to the intrahepatic oxidation-reduction state in the septic model.
Iimuro
Y
Y
First Department of Surgery, Yamanashi Medical College, Japan.
Yamamoto
M
M
Inoue
S
S
Kohno
H
H
Matsumoto
Y
Y
D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001158:Arteries; D001663:Bilirubin / Q000097:blood; D004195:Disease Models, Animal; D004927:Escherichia coli Infections / Q000378:metabolism; D066298:In Vitro Techniques; D007657:Ketone Bodies / Q000097:blood*; D007728:Kupffer Cells / Q000378:metabolism*; D008264:Macrophages / Q000378:metabolism; D008297:Male; D008930:Mitochondria, Liver / Q000378:metabolism; D010084:Oxidation-Reduction; D051381:Rats; D017207:Rats, Sprague-Dawley; D018805:Sepsis / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism*
D007657:Ketone Bodies; D013481:Superoxides; D000410:Alanine Transaminase; D001663:Bilirubin