gamma-Aminobutyric acid receptor activation of outer hair cells in the guinea pig cochlea.
Gamma-aminobutyric acid (GABA), the main inhibitory neurotransmitter of the central nervous system, may also be released from olivocochlear efferent nerves reaching the outer hair cells of the cochlea. In the present study the cell potential of isolated outer hair cells of the third and fourth turns of the guinea pig cochlea was measured with patch-clamp electrodes. GABA-receptor agonists and antagonists were applied extracellularly. The cell membrane hyperpolarized in a reversible manner with increasing concentrations of extracellular GABA. Half-maximal hyperpolarization (2 mV) was achieved with approximately 10(-7) M GABA. Desensitization was not observed. The hyperpolarizing effect of GABA was potentiated by the benzodiazepine clorazepate and was blocked by picrotoxin.
Gitter
A H
AH
Department of Otolaryngology, University of Tübingen, Federal Republic of Germany.
Zenner
H P
HP
D000818:Animals; D006168:Guinea Pigs; D006198:Hair Cells, Auditory / Q000502:physiology*; D008564:Membrane Potentials; D011963:Receptors, GABA-A / Q000502:physiology*
D011963:Receptors, GABA-A