Effect of rufloxacin on in-vitro proliferation and differentiation of human mononuclear cells.
We investigated the effects of rufloxacin, a new, long acting fluoroquinolone, on the growth and differentiation of human peripheral blood mononuclear cells (MNC) stimulated with T- and B-cell mitogenic agents. Rufloxacin inhibited 3H-thymidine incorporation into MNC stimulated with phytohaemagglutinin (PHA) and pokeweed mitogen (PWM) in a dose-dependent manner. The concentrations of rufloxacin required to inhibit 1/2 maximal proliferation of T- and B-cells were 62 and 33.5 mg/L respectively. Rufloxacin, at clinically achievable serum levels (less than 10 mg/L), was found not to inhibit PHA-induced T-cell differentiation as assessed by IL-2 production, IL-2 receptor expression and the expression of cell differentiation markers (CD4 and CD8). However, higher concentrations of rufloxacin (10 and 50 mg/L) markedly inhibited B-cell differentiation in-vitro as determined by the measurement of immunoglobulin production by MNC stimulated with PWM. The clinical relevance of our in-vitro findings remains to be elucidated.
Gollapudi
S
S
Department of Basic and Clinical Immunology, University of California, Irvine.
Perumal
V
V
Thadepalli
H
H
D000890:Anti-Infective Agents / Q000494:pharmacology*; D001402:B-Lymphocytes / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D024841:Fluoroquinolones; D006801:Humans; D007074:Immunoglobulin G / Q000096:biosynthesis; D066298:In Vitro Techniques; D008934:Mitogens / Q000494:pharmacology; D009000:Monocytes / Q000187:drug effects*; D010835:Phytohemagglutinins; D015363:Quinolones / Q000494:pharmacology*; D011971:Receptors, Immunologic / Q000187:drug effects; D017472:Receptors, Interleukin-1; D015375:Receptors, Interleukin-2 / Q000187:drug effects; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology
D000890:Anti-Infective Agents; D024841:Fluoroquinolones; D007074:Immunoglobulin G; D008934:Mitogens; D010835:Phytohemagglutinins; D015363:Quinolones; D011971:Receptors, Immunologic; D017472:Receptors, Interleukin-1; D015375:Receptors, Interleukin-2; C060328:rufloxacin