Expression of Fos-like immunoreactivity by yohimbine and clonidine in the rat brain.
To elucidate the role of alpha 2-adrenoceptors in transcriptional control in the rat brain, we localized the Fos-like immunoreactivity (Fos-LI) induced by alpha 2-adrenoceptor agonists and by an antagonist. Injections of yohimbine (5 mg/kg, i.p.) into rats led to the induction of Fos-LI in areas with a dense alpha 2-adrenoceptor binding such as the locus coeruleus, the bed nucleus of stria terminalis, the central nucleus of amygdaloid complex, the paraventricular nucleus, the nucleus tractus solitarius, and ventrolateral medulla oblongata. Clonidine (500 micrograms/kg, i.p.) suppressed the Fos expression by yohimbine in these nuclei, and clonidine (100 micrograms/kg, i.p.) or guanabenz (4 mg/kg, i.p.) induced Fos-LI in oxytocin neurons in the paraventricular and supraoptic nuclei in the hypothalamus. Thus, the alpha 2-adrenoceptor is involved in transcriptional control via Fos expression in neurons related to autonomic and other functions.
Tsujino
T
T
First Department of Internal Medicine, Kobe University School of Medicine, Japan.
Sano
H
H
Kubota
Y
Y
Hsieh
S T
ST
Miyajima
T
T
Saito
K
K
Nakajima
M
M
Saito
N
N
Yokoyama
M
M
D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D003000:Clonidine / Q000494:pharmacology*; D016762:Genes, fos; D006143:Guanabenz / Q000494:pharmacology; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D008297:Male; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D016760:Proto-Oncogene Proteins c-fos / Q000032:analysis / Q000096:biosynthesis* / Q000276:immunology; D051381:Rats; D017208:Rats, Wistar; D011942:Receptors, Adrenergic, alpha / Q000378:metabolism*; D015016:Yohimbine / Q000494:pharmacology*
D016760:Proto-Oncogene Proteins c-fos; D011942:Receptors, Adrenergic, alpha; D015016:Yohimbine; D006143:Guanabenz; D003000:Clonidine