Detection of DNA aberrations in human cancers by single-strand conformation polymorphism analysis of polymerase chain reaction products.
We have developed a simple, sensitive method, single-strand conformation polymorphism (SSCP) analysis, to detect a single nucleotide substitution in a DNA fragment amplified and labeled by the polymerase chain reaction (PCR). Mobility shift of single-stranded DNAs due to their specific conformations on non-denaturing polyacrylamide gel electrophoresis can reveal DNA aberrations. By the PCR-SSCP analysis of DNAs from surgical specimens of human cancers, mutated ras genes (17%) and aberrations of tumor suppressor p53 gene (53%) including loss of one of the two alleles and a mutation in the remaining allele were detected in lung carcinomas and aberrations of both of the p53 and retinoblastoma (RB) genes were detected exclusively in advanced hepatocellular carcinomas.
Murakami
Y
Y
Oncogene Division, National Cancer Center Research Institute, Tokyo, Japan.
Suzuki
Y
Y
Kishimoto
Y
Y
Hirohashi
S
S
Hayashi
K
K
Sekiya
T
T
D006528:Carcinoma, Hepatocellular / Q000235:genetics; D002869:Chromosome Aberrations; D004273:DNA, Neoplasm / Q000235:genetics*; D004277:DNA, Single-Stranded / Q000235:genetics*; D016158:Genes, p53; D011905:Genes, ras; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics; D008175:Lung Neoplasms / Q000235:genetics; D009154:Mutation; D009690:Nucleic Acid Conformation; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012175:Retinoblastoma / Q000235:genetics
D004273:DNA, Neoplasm; D004277:DNA, Single-Stranded