title: Further diagnostic use of an invasive-specific monoclonal antibody against Entamoeba histolytica.
abstract: Genotypic differences between invasive and non-invasive E. histolytica could explain the 1:10 ratio of symptomatic/asymptomatic infection worldwide. Currently, zymodeme analysis is used to differentiate invasive from non-invasive E. histolytica strains but the technique is cumbersome and expensive. In accordance with the WHO research priorities for amebiasis we report here the further use of an invasive-specific monoclonal antibody against E. histolytica in immunofluorescence, to identify isolates cultured from stool samples of patients from three geographically distant endemic regions: Bangladesh, Colombia and Mexico. We tested 107 E. histolytica isolates and the correlation between zymodeme characterization and the immunofluorescence assay was 100%.
authors.affiliation: Department of Medical Parasitology, London School of Hygiene and Tropical Medicine, UK.
authors.forename: A
authors.initials: A
authors.lastname: González-Ruíz
authors.forename: R
authors.initials: R
authors.lastname: Haque
authors.forename: T
authors.initials: T
authors.lastname: Rehman
authors.forename: A
authors.initials: A
authors.lastname: Aguirre
authors.forename: G
authors.initials: G
authors.lastname: Castañón
authors.forename: A
authors.initials: A
authors.lastname: Hall
authors.forename: F
authors.initials: F
authors.lastname: Guhl
authors.forename: G
authors.initials: G
authors.lastname: Ruíz-Palacios
authors.forename: D C
authors.initials: DC
authors.lastname: Warhurst
authors.forename: M A
authors.initials: MA
authors.lastname: Miles
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000913:Antibodies, Protozoan / Q000276:immunology*; D001459:Bangladesh / Q000453:epidemiology; D003105:Colombia / Q000453:epidemiology; D004404:Dysentery, Amebic / Q000453:epidemiology / Q000469:parasitology*; D004748:Entamoeba histolytica / Q000201:enzymology / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification / Q000472:pathogenicity; D005243:Feces / Q000469:parasitology; D005455:Fluorescent Antibody Technique; D007527:Isoenzymes / Q000032:analysis; D008800:Mexico / Q000453:epidemiology; D015800:Protozoan Proteins / Q000032:analysis; D014774:Virulence
chemical_list: D000911:Antibodies, Monoclonal; D000913:Antibodies, Protozoan; D007527:Isoenzymes; D015800:Protozoan Proteins