Transcriptional regulation of the plastocyanin and cytochrome c553 genes from the cyanobacterium Anabaena species PCC 7937.
The effect of copper on the levels of plastocyanin (PC) and cytochrome c553 (cyt-c)-specific transcripts from Anabaena sp. PCC 7937 was investigated. The addition of copper resulted in a marked increase in PC mRNA levels, and a decrease in cyt c mRNA levels. Thus the functional exchange between PC and cyt c seems to be regulated at the mRNA level. The copper-dependent increase in PC and decrease in cyt c mRNA levels was abolished when chloramphenicol was added to the cells. This suggests that de novo synthesis of at least one trans-acting element is required to regulate PC and cyt c mRNA levels. Both PC and cyt c mRNA stability was found to be unaltered under varying Cu2+ regimes. This leads to the conclusion that expression of both genes is regulated at the level of initiation of transcription.
Bovy
A
A
Department of Molecular Cell Biology, State University of Utrecht, The Netherlands.
de Vrieze
G
G
Borrias
M
M
Weisbeek
P
P
D017033:Anabaena / Q000187:drug effects / Q000378:metabolism*; D001483:Base Sequence; D002701:Chloramphenicol / Q000494:pharmacology; D003300:Copper / Q000378:metabolism* / Q000494:pharmacology; D003574:Cytochrome c Group / Q000096:biosynthesis* / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects / Q000502:physiology; D007700:Kinetics; D008969:Molecular Sequence Data; D010970:Plastocyanin / Q000096:biosynthesis* / Q000235:genetics; D014176:Protein Biosynthesis / Q000187:drug effects; D012333:RNA, Messenger / Q000032:analysis; D012293:Rifampin / Q000494:pharmacology; D014158:Transcription, Genetic / Q000187:drug effects / Q000502:physiology*
D003574:Cytochrome c Group; D012333:RNA, Messenger; C083951:cytochrome c553; D002701:Chloramphenicol; D003300:Copper; D010970:Plastocyanin; C022302:cytochrome C-552; D012293:Rifampin