title: High frequency of transmission of murine AIDS virus in C57BL/10 mice via mother's milk.
abstract: Maternal transmission of a murine leukemia virus (MuLV) mixture named LP-BM5 MuLV, which is knwon to induce murine AIDS (MAIDS), was investigated. Adult female C57BL/10 mice were inoculated intraperitoneally with LP-BM5 MuLV. When the virus-inoculated female mice developed splenomegaly or lymphadenopathy, they were mated with normal C57BL/10 male mice. Of 56 offspring born to MAIDS mothers, 14 appeared to develop MAIDS, as assessed by the occurrence of splenomegaly or lymphadenopathy as well as the mitogen response of spleen cells. The occurrence of MAIDS in offspring was found to be accompanied by the maternal transmission and expansion of a defective virus genome from which almost the entire pol and env regions are deleted. On the other hand, the ecotropic helper virus genome was detected in all offspring regardless of the occurrence of MAIDS. To examine the mode of maternal transmission of LP-BM5 MuLV, foster-nursing experiments were conducted. The ecotropic helper viruses were found in all normal offspring nursed by a MAIDS mother, and some of them developed MAIDS. In contrast, none of offspring born to a MAIDS mother that were nursed by an uninfected foster mother either carried the LP-BM5 MuLV or developed MAIDS. Finally, both the defective and the ecotropic helper viruses were detected in LP-BM5 MuLV-infected mother's milk. These results indicated that maternal transmission of LP-BM5 MuLV occurs with a high frequency and is mediated by mother's milk.
authors.affiliation: Department of Blood Products, National Institute of Health, Tokyo, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Okada
authors.forename: K
authors.initials: K
authors.lastname: Suzuki
authors.forename: K
authors.initials: K
authors.lastname: Komuro
authors.forename: T
authors.initials: T
authors.lastname: Mizuochi
mesh_terms: D000818:Animals; D001483:Base Sequence; D003673:Defective Viruses / Q000302:isolation & purification / Q000472:pathogenicity*; D004195:Disease Models, Animal; D005260:Female; D015752:Genes, env / Q000235:genetics; D015751:Genes, pol / Q000235:genetics; D016679:Genome, Viral; D006378:Helper Viruses / Q000302:isolation & purification; D009052:Leukemia Virus, Murine / Q000302:isolation & purification / Q000472:pathogenicity*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008892:Milk / Q000382:microbiology*; D008969:Molecular Sequence Data; D016183:Murine Acquired Immunodeficiency Syndrome / Q000635:transmission*; D011247:Pregnancy; D014774:Virulence