title: Phorbol ester-induced changes in rat hippocampal glycoprotein fucosylation.
abstract: Studies on glycoprotein fucosylation were carried out using hippocampal slices from rat brain. These slices were incubated in the presence of the protein kinase C (PKC) activating phorbol ester, 4 beta-phorbol-12,13-dibutyrate (PDBu), or an inactive isoform, 4 alpha-phorbol-12,13-didecanoate (PDD), respectively, for 7 min followed by a 60 min pulse of [3H]fucose. PDBu caused an increase in [3H]fucose incorporation into glycoproteins by 29% as well as an activation of the fucokinase enzyme reaction by 21%. The PDBu-induced stimulation of [3H]fucose insertion into hippocampal glycoproteins was abolished by the PKC inhibitors, staurosporine and H7. The importance of a PKC-regulated glycoprotein fucosylation in mechanisms underlying changes in neuronal plasticity is discussed.
authors.affiliation: Institute of Neurobiology and Brain Research Magdeburg, F.R.G.
authors.forename: F
authors.initials: F
authors.lastname: Angenstein
authors.forename: S
authors.initials: S
authors.lastname: Staak
authors.forename: R
authors.initials: R
authors.lastname: Jork
mesh_terms: D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D000470:Alkaloids / Q000494:pharmacology; D000818:Animals; D005643:Fucose / Q000378:metabolism*; D006023:Glycoproteins / Q000096:biosynthesis*; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism*; D066298:In Vitro Techniques; D007546:Isoquinolines / Q000494:pharmacology; D009419:Nerve Tissue Proteins / Q000096:biosynthesis*; D015240:Phorbol 12,13-Dibutyrate / Q000494:pharmacology*; D010770:Phosphotransferases / Q000037:antagonists & inhibitors; D017853:Phosphotransferases (Alcohol Group Acceptor); D010879:Piperazines / Q000494:pharmacology; D011493:Protein Kinase C / Q000037:antagonists & inhibitors; D051381:Rats; D019311:Staurosporine
chemical_list: D000470:Alkaloids; D006023:Glycoproteins; D007546:Isoquinolines; D009419:Nerve Tissue Proteins; D010879:Piperazines; D005643:Fucose; D015240:Phorbol 12,13-Dibutyrate; D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D010770:Phosphotransferases; D017853:Phosphotransferases (Alcohol Group Acceptor); C020153:fucokinase; D011493:Protein Kinase C; D019311:Staurosporine