Proteinkinase C beta-isoform triggers the formation of prostanoids and superoxide in liver macrophages.
The zymosan- and phorbolester-induced formation of prostanoids in cultured rat liver macrophages has been shown recently to be controlled by proteinkinase C (1). Using specific antibodies raised against the alpha-, beta-, gamma- and epsilon-isoforms of proteinkinase C, we show that proteinkinase-beta is the predominant isoform in rat liver macrophages. Northern blot analysis with a beta-isoform-specific c-DNA probe revealed the expression of m-RNA for proteinkinase-beta. In resting cells the beta-isoform of proteinkinase C is nearly equally distributed between the cytosolic and membrane fractions. Zymosan and phorbolester led to a translocation of proteinkinase-beta from the cytosol to the membranes, whereas exogenously added arachidonic acid and the calcium ionophore A23187 had no effect. These data indicate that the beta-isoform of proteinkinase C takes part in the prostaglandin and superoxide formation following PMA and zymosan treatment of rat liver macrophages.
Duyster
J
J
Institut für Molekulare Zellbiologie, Ludwigs-Universität, Freiburg, FRG.
Hidaka
H
H
Decker
K
K
Dieter
P
P
D000818:Animals; D000001:Calcimycin / Q000494:pharmacology; D002478:Cells, Cultured; D015232:Dinoprostone / Q000378:metabolism; D007527:Isoenzymes / Q000378:metabolism; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D008264:Macrophages / Q000378:metabolism*; D008297:Male; D011453:Prostaglandins / Q000096:biosynthesis*; D011493:Protein Kinase C / Q000378:metabolism*; D051381:Rats; D015398:Signal Transduction; D013481:Superoxides / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D015054:Zymosan / Q000494:pharmacology
D007527:Isoenzymes; D011453:Prostaglandins; D013481:Superoxides; D000001:Calcimycin; D015054:Zymosan; D011493:Protein Kinase C; D015232:Dinoprostone; D013755:Tetradecanoylphorbol Acetate