Copper-mediated regulation of cytochrome c553 and plastocyanin in the cyanobacterium Synechocystis 6803.
In certain cyanobacteria and algae, cytochrome c553 or plastocyanin can serve to carry electrons from the cytochrome bf complex to photosystem I. The availability of copper in the growth medium regulates which protein is present. To investigate copper induced control of gene expression we isolated these proteins from the cyanobacterium Synechocystis 6803. Using immunodetection and optical spectroscopy, the steady state levels of cytochrome c553 and plastocyanin were measured in cells grown at different copper concentrations. The results show that in cells grown in 20-30 nM copper, cytochrome c553 was present, whereas plastocyanin was not detected. The opposite behavior was observed in cells grown in the presence of 1 microM copper; plastocyanin was present, whereas cytochrome c553 could not be detected. Both proteins were present in cells grown in 0.3 microM copper. Northern analysis of total RNA, probed with a gene fragment for cytochrome c553 or the plastocyanin gene, showed that cells grown in the presence of 20-30 nM copper have message for cytochrome c553, but not for plastocyanin, whereas cells grown in 1 microM copper have message for plastocyanin, but not for cytochrome c553. These results demonstrate that copper regulates expression of both of the genes encoding cytochrome c553 and plastocyanin prior to translation in Synechocystis 6803.
Zhang
L
L
Department of Plant Biology, University of Illinois, Urbana.
McSpadden
B
B
Pakrasi
H B
HB
Whitmarsh
J
J
D003300:Copper / Q000378:metabolism*; D000458:Cyanobacteria / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D003574:Cytochrome c Group / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000528:radiation effects; D008027:Light; D010970:Plastocyanin / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics
D003574:Cytochrome c Group; D012333:RNA, Messenger; C083951:cytochrome c553; D003300:Copper; D010970:Plastocyanin; C022302:cytochrome C-552