Polarization sensitive coherent anti-Stokes Raman scattering spectroscopy of the amide I band of proteins in solutions.
Polarization sensitive coherent anti-Stokes Raman scattering (PCARS) spectroscopy is a fruitful technique to study Raman vibrations of diluted molecules under off-electron resonant conditions. We apply PCARS as a direct spectroscopic method to investigate the broad amide I band of proteins in heavy water. In spontaneous Raman spectroscopy, this band is not well resolved. We fit a number of spectra taken of each protein under different polarization conditions, with a single set of parameters. It then appears that some substructure is observed in the amide I band. From this substructure, we determine the percentage of alpha-helix, beta-sheet, and random coil for the proteins lysozyme, albumin, ribonuclease A, and alpha-chymotrypsin.
Chikishev
A Y
AY
International Laser Center, Moscow State University, Russia.
Lucassen
G W
GW
Koroteev
N I
NI
Otto
C
C
Greve
J
J
D000577:Amides; D000818:Animals; D002417:Cattle; D002645:Chickens; D002918:Chymotrypsin / Q000737:chemistry; D003903:Deuterium; D017666:Deuterium Oxide; D006801:Humans; D008433:Mathematics; D008962:Models, Theoretical; D009113:Muramidase / Q000737:chemistry; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry*; D012259:Ribonuclease, Pancreatic / Q000737:chemistry; D012709:Serum Albumin / Q000737:chemistry; D013059:Spectrum Analysis, Raman / Q000379:methods; D014867:Water
D000577:Amides; D011506:Proteins; D012709:Serum Albumin; D014867:Water; D003903:Deuterium; D012259:Ribonuclease, Pancreatic; D009113:Muramidase; D002918:Chymotrypsin; D017666:Deuterium Oxide