Biosynthesis of paf-acether in cultured-mouse mast cells: the role of calcium and G proteins.
We examined the potential role of a guanine nucleotide-binding protein in the biosynthesis of paf-acether (paf) and the release of beta-hexosaminidase during antigenic stimulation of cultured mouse bone marrow-derived mast cells. Unlike pertussis toxin, cholera toxin treatment enhanced the antigen-stimulated production of paf and calcium mobilisation without affecting acetyltransferase activation and cell degranulation. The level of intracellular cAMP doubled in cholera toxin-treated cells. Our data suggest that a cholera toxin-sensitive guanine nucleotide-binding protein is involved in the IgE receptor-mediated signal transduction leading to paf production most probably at the level of Ca2+ influx.
Joly
F
F
INSERM U 200, Université Paris-Sud, Clamart, France.
Beauvais
F
F
Ninio
E
E
D000123:Acetyltransferases / Q000378:metabolism; D000818:Animals; D000944:Antigens, Differentiation, B-Lymphocyte / Q000187:drug effects / Q000502:physiology; D001853:Bone Marrow / Q000378:metabolism; D001854:Bone Marrow Cells; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D002772:Cholera Toxin / Q000494:pharmacology; D000242:Cyclic AMP / Q000378:metabolism; D019204:GTP-Binding Proteins / Q000378:metabolism*; D007073:Immunoglobulin E / Q000378:metabolism; D007700:Kinetics; D008407:Mast Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D037342:Pertussis Toxin; D010972:Platelet Activating Factor / Q000096:biosynthesis*; D011961:Receptors, Fc / Q000187:drug effects / Q000502:physiology; D017455:Receptors, IgE; D015398:Signal Transduction / Q000187:drug effects; D010566:Virulence Factors, Bordetella / Q000494:pharmacology; D001619:beta-N-Acetylhexosaminidases / Q000378:metabolism
D000944:Antigens, Differentiation, B-Lymphocyte; D010972:Platelet Activating Factor; D011961:Receptors, Fc; D017455:Receptors, IgE; D010566:Virulence Factors, Bordetella; D007073:Immunoglobulin E; D002772:Cholera Toxin; D000242:Cyclic AMP; D000123:Acetyltransferases; D037342:Pertussis Toxin; D001619:beta-N-Acetylhexosaminidases; D019204:GTP-Binding Proteins; D002118:Calcium