Differentiation of quail myoblasts transformed with a temperature sensitive mutant of Rous sarcoma virus. II. Relationship of myoblast fusion with calcium and temperature.
The effects of calcium and temperature on fusion of quail embryonic myoblasts were examined using cells transformed with a temperature-sensitive mutant of Rous sarcoma virus (ts-RSV). The transformed quail myoblasts (QM-RSV) fused to form myotubes at 41 degrees C, the non-permissive temperature, but not at 35.5 degrees C, the permissive temperature. On incubation at 41 degrees C, a period of more than 10 hr was needed for the myoblasts to become fusion-competent, but calcium was not needed for development of fusion-competence. Once the cells had become competent, fusion proceeded even at 35.5 degrees C. These results suggest that the src gene product expressed at 35.5 degrees C may control the fusion of cells in the competent stage by inactivating a component(s) that is associated with fusion-competence. However, fusion of even myoblasts in the competent stage was blocked in calcium-deficient medium, suggesting that calcium is essential for the fusion, probably at a step immediately before membrane union. Unlike fusion, other biochemical processes of differentiation proceeded even in calcium-deficient medium, indicating a distinction of fusion from these other processes during myoblast differentiation.
Kim
J
J
Institute of Molecular and Cellular Biology for Pharmaceutical Sciences, Kyoto Pharmaceutical University, Japan.
Adachi
T
T
Saiuchi
M
M
Asada
A
A
D000818:Animals; D001358:Avian Sarcoma Viruses / Q000235:genetics; D002118:Calcium / Q000378:metabolism*; D002454:Cell Differentiation / Q000235:genetics; D002459:Cell Fusion / Q000235:genetics*; D002461:Cell Line, Transformed; D003370:Coturnix; D016391:Genes, src / Q000502:physiology*; D008561:Membrane Fusion / Q000502:physiology; D009132:Muscles / Q000196:embryology*; D011505:Protein-Tyrosine Kinases / Q000502:physiology*; D013696:Temperature
D011505:Protein-Tyrosine Kinases; D002118:Calcium