title: Neurotransmitter-stimulated immediate-early gene responses are organized through differential post-synaptic receptor mechanisms.
abstract: The products of the cellular immediate-early genes (IEGs) are thought to act as messengers in the coupling of trans-synaptic stimuli with altered neuronal gene expression. However, the manner in which neurotransmission specifies particular responses through the IEGs is undefined. In this report, mRNA and transcription analysis of a precisely-timed, physiological IEG response illustrates how an IEG signal may be organized through differential neurotransmitter receptor activation. The nocturnal pattern of IEG expression in the rat pineal gland has been shown to be differentially regulated through post-synaptic adrenergic receptors. Induction of the c-fos gene is primarily mediated through alpha 1-receptors, whereas the coordinately regulated jun-B gene exhibits dual regulation through alpha 1- and beta-receptors. A simultaneous repression of c-jun expression is partly mediated through a beta-receptor mechanism. In vitro analysis of IEGs in cultured pineal glands has confirmed the receptor-specific link between adrenergic neurotransmission and IEG induction. The pineal is a unique neuroendocrine model in which the characteristics and function of the IEG third messenger system may be defined.
authors.affiliation: Neuropeptide Laboratory, National University of Singapore.
authors.forename: D A
authors.initials: DA
authors.lastname: Carter
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D046508:Culture Techniques; D016762:Genes, fos / Q000235:genetics; D016758:Genes, jun / Q000235:genetics; D008297:Male; D010870:Pineal Gland / Q000187:drug effects*; D011224:Prazosin / Q000494:pharmacology; D011433:Propranolol / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D017981:Receptors, Neurotransmitter / Q000187:drug effects*; D013569:Synapses / Q000187:drug effects*; D009435:Synaptic Transmission / Q000187:drug effects*; D014158:Transcription, Genetic / Q000187:drug effects*
chemical_list: D017981:Receptors, Neurotransmitter; D011433:Propranolol; D011224:Prazosin