[Evaluation of IgG-IgM antibodies against the Epstein-Barr virus nuclear antigen (EBNA-1) using ELISA].
BACKGROUND
Evaluation of two commercial immunoenzymatic techniques (ELISA) for detection of anti-EBNA-1 IgG and IgM for diagnosis of Epstein-Barr virus mononucleosis.


METHODS
Study of anti-EBNA-1 IgG and IgM in infectious mononucleosis and they have been compared with the detection of IgM antibodies to VCA by indirect immunofluorescence as the reference method.


RESULTS
The sensitivity of the assay was 87.5%, the specificity was 89.4%, the positive predictive value was 91.4% and the negative predictive value was 85%.


CONCLUSIONS
This technique can be useful in laboratories without the necessary equipment and experienced personnel for indirect immunofluorescence. It also can be used as a complementary determination to the anti-VCA IgM in the long lasting infectious mononucleosis, when this marker is negative.
Martínez Zapico
R
R
Servicio de Microbiología, Hospital La Paz, Madrid.
Sobejano
L
L
Llamazares
I
I
Ladrón de Guevara
C
C
D000914:Antibodies, Viral / Q000097:blood*; D000956:Antigens, Viral / Q000276:immunology*; D015415:Biomarkers; D036022:Capsid Proteins; D004268:DNA-Binding Proteins / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D019309:Epstein-Barr Virus Nuclear Antigens; D004854:Herpesvirus 4, Human / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood*; D007075:Immunoglobulin M / Q000097:blood*; D007244:Infectious Mononucleosis / Q000175:diagnosis*; D011237:Predictive Value of Tests; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity
D000914:Antibodies, Viral; D000956:Antigens, Viral; D015415:Biomarkers; D036022:Capsid Proteins; D004268:DNA-Binding Proteins; D019309:Epstein-Barr Virus Nuclear Antigens; C041788:Epstein-Barr viral capsid antigen; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D011933:Reagent Kits, Diagnostic
Estudio y valoración de anticuerpos IgG-IgM frente al antígeno nuclear del virus de Epstein-Barr (EBNA-1) mediante ELISA.