title: Site-specific incorporation of [125I]iododeoxyuridine into DNA.
abstract: A procedure for the incorporation of [125I]IdU into specific sites in DNA is described. The approach depends upon attachment of radioiododeoxyuridine to a controlled pore glass support which is then used for automated synthesis of an oligomer. The resulting oligomer, containing a terminal 3'[125I]iododeoxyuridine, is used as a primer during DNA synthesis catalyzed by the Taq polymerase employing thermal cycling. The product formed includes the radioiodonucleotide at a single internal site determined by the length of the oligomer.
authors.affiliation: University of Chicago Hospitals and Clinics, IL.
authors.forename: N
authors.initials: N
authors.lastname: Scherberg
authors.forename: I
authors.initials: I
authors.lastname: Bloch
authors.forename: P
authors.initials: P
authors.lastname: Gardner
mesh_terms: D001483:Base Sequence; D001665:Binding Sites; D004247:DNA / Q000737:chemistry*; D007065:Idoxuridine / Q000737:chemistry*; D007457:Iodine Radioisotopes; D008969:Molecular Sequence Data
chemical_list: D007457:Iodine Radioisotopes; D004247:DNA; D007065:Idoxuridine