The relationship between endogenous oestradiol and vitamin D3 metabolites in serum and follicular fluid during ovarian stimulation for in-vitro fertilization and embryo transfer.
The present study was undertaken to examine the effect of circulating oestradiol on serum levels of 25-hydroxyvitamin D3 (25-OHD3), 24,25-dihydroxyvitamin D3[24,25-(OH)2D3], and 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3] during gonadotrophin-induced ovarian stimulation in 10 healthy women undergoing in-vitro fertilization and embryo transfer (IVF). The presence of these metabolites in the follicular fluid was also investigated. Plasma oestradiol increased from 25 +/- 3.2 (mean +/- SE) pg/ml before initiation of treatment to 2563 +/- 328 pg/ml on the day of injection of human chorionic gonadotrophin (HCG) and 1641 +/- 299 pg/ml on the day of ovum retrieval (P < 0.01). Serum levels of 1,25-(OH)2D3 increased from 32.0 +/- 1.9 (mean +/- SE) pg/ml to 46.6 +/- 8.1 and 48.5 +/- 7.7 pg/ml (P < 0.05) on the day of HCG and ovum retrieval, respectively. No changes in blood levels of 25-OHD3 and 24,25-(OH)2D3 were found. The presence of vitamin D metabolites in follicular fluid is documented herein for the first time. All three metabolites were present in the follicular fluid but were significantly lower than in the concurrent serum (P < 0.01). A highly significant correlation was found between serum and follicular fluid levels: r = 0.787, P < 0.001 for 1,25-(OH)2D3; r = 0.738, P < 0.01 for 25-OHD3; and r = 0.751, P < 0.01 for 24,25-(OH)2D3. Our results suggest that raised levels of circulating oestradiol during gonadotrophin-induced ovarian stimulation are associated with a significant increase of serum 1,25-(OH)2D3.
Potashnik
G
G
Fertility and IVF Unit, Toor Institute, Haifa, Israel.
Lunenfeld
E
E
Levitas
E
E
Itskovitz
J
J
Albutiano
S
S
Yankowitz
N
N
Sonin
Y
Y
Levy
J
J
Glezerman
M
M
Shany
S
S
D015650:24,25-Dihydroxyvitamin D 3 / Q000032:analysis; D000328:Adult; D002112:Calcifediol / Q000032:analysis; D002117:Calcitriol / Q000032:analysis; D002762:Cholecalciferol / Q000378:metabolism*; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D004624:Embryo Transfer; D004958:Estradiol / Q000097:blood*; D005260:Female; D005307:Fertilization in Vitro; D015571:Follicular Fluid / Q000737:chemistry*; D006801:Humans; D010062:Ovulation Induction / Q000379:methods*; D012044:Regression Analysis; D017329:Triptorelin Pamoate / Q000627:therapeutic use
D006063:Chorionic Gonadotropin; D017329:Triptorelin Pamoate; D002762:Cholecalciferol; D015650:24,25-Dihydroxyvitamin D 3; D004958:Estradiol; D002117:Calcitriol; D002112:Calcifediol