Human papillomavirus detection in cervical dysplasias or neoplasias and in condylomata acuminata by in situ hybridization with biotinylated DNA probes.
Specimens from cervical dysplasias or carcinomas and genital condylomata acuminata were retrospectively analysed by in situ hybridization (ISH) with biotinylated DNA probes for human papillomavirus (HPV) types 6, 11, 16 and 18. In the control group no case was positive for HPV DNA. In mild/moderate dysplasias, 4 cases (14%) were positive for HPV 6 or 11 and 2 cases (7%), for HPV 16. In the severe dysplasia/in situ carcinoma group, 9 cases (31%) showed presence of DNA of HPV types 16 or 18. Six invasive carcinomas (20%) were positive for HPV type 16 or 18. Among condylomata acuminata, 22 cases (73%) were positive for HPV types 6 or 11. In all ISH-positive cases only one viral type was detected. No correlation between HPV DNA positivity and histological findings of HPV infection was observed. Although less sensitive than some other molecular biology techniques, in situ hybridization with biotinylated DNA probes proved to be simple and useful for detecting and typing HPV in samples routinely received for histopathological analysis.
Guimarães
E M
EM
Departamento de Anatomia Patológica e Medicina Legal, Faculdade de Medicina, UFMG, Belo Horizonte, Brasil.
Brasileiro Filho
G
G
Pena
S D
SD
D001710:Biotin; D001938:Brazil / Q000453:epidemiology; D002294:Carcinoma, Squamous Cell / Q000453:epidemiology / Q000235:genetics*; D003218:Condylomata Acuminata / Q000453:epidemiology / Q000235:genetics*; D015344:DNA Probes, HPV; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D006801:Humans; D017403:In Situ Hybridization / Q000379:methods* / Q000706:statistics & numerical data; D027383:Papillomaviridae / Q000235:genetics*; D015995:Prevalence; D012189:Retrospective Studies; D002577:Uterine Cervical Diseases / Q000453:epidemiology / Q000235:genetics*; D002578:Uterine Cervical Dysplasia / Q000453:epidemiology / Q000235:genetics*; D002583:Uterine Cervical Neoplasms / Q000453:epidemiology / Q000235:genetics*
D015344:DNA Probes, HPV; D004279:DNA, Viral; D001710:Biotin