[Direct in vivo gene introduction into rat kidney].
We established a simple and efficient method in vivo gene transfer using Sendai virus (HVJ) and liposomes. Plasmid DNA and high mobility group 1 (MHG1) protein were co-encapsulated in liposomes and co-introduced into target cells by HVJ-mediated membrane fusion. pAct-SVT DNA, as a reporter gene, was introduced into the kidney of intact rats through a cannula in the renal artery, and SV 40 large T antigen was detected by enzyme immunohistochemically in glomerular cells (mesangial cells and/or capillary cells) 4 days after it's introduction. Thereafter expression of SV 40 large T antigen gene decreased and was not detected on day 10. This gene delivery system could be useful in research and development of gene therapy of renal disease.
Tomita
N
N
Dept. of Geriatric Medicine, Osaka University Medical School.
Higaki
J
J
Kaneda
Y
Y
Ogihara
T
T
D000818:Animals; D000952:Antigens, Polyomavirus Transforming; D004247:DNA; D007678:Kidney Glomerulus / Q000166:cytology*; D008081:Liposomes; D008297:Male; D008561:Membrane Fusion; D010222:Parainfluenza Virus 1, Human / Q000235:genetics*; D051381:Rats; D017208:Rats, Wistar; D014162:Transfection / Q000379:methods*
D000952:Antigens, Polyomavirus Transforming; D008081:Liposomes; D004247:DNA