title: Proton relaxation enhancement by means of serum albumin and poly-L-lysine labeled with DTPA-Gd3+: relaxivities as a function of molecular weight and conjugation efficiency.
abstract: A series of poly-L-lysine chains, with molecular weight ranging from 3300 up to 102,000 Da, were labeled with DTPA-Gd3+. No significant differences in longitudinal and transversal relaxivity, could be demonstrated as a function of the chain length. The R1 and R2 relaxivities were respectively 2.5 and 5 times superior to those of plain DTPA-Gd3+ (at 2.4 T). Bovine serum albumin was also labeled in a way that a wide (DTPA-Gd3+)/BSA range (3-39) was obtained. The longitudinal relaxivity, of these paramagnetically labeled albumins, increased with increasing (DTPA-Gd3+)/BSA ratios. This effect was most pronounced at very low (DTPA-Gd3+)/BSA ratios.
authors.affiliation: Department of Organic Chemistry, University of Antwerp (RUCA), Belgium.
authors.forename: M
authors.initials: M
authors.lastname: Spanoghe
authors.forename: D
authors.initials: D
authors.lastname: Lanens
authors.forename: R
authors.initials: R
authors.lastname: Dommisse
authors.forename: A
authors.initials: A
authors.lastname: Van der Linden
authors.forename: F
authors.initials: F
authors.lastname: Alderweireldt
mesh_terms: D019786:Gadolinium DTPA; D007553:Isotope Labeling / Q000379:methods; D009682:Magnetic Resonance Spectroscopy; D008970:Molecular Weight; D009942:Organometallic Compounds; D004369:Pentetic Acid; D011107:Polylysine; D011522:Protons; D012710:Serum Albumin, Bovine
chemical_list: D009942:Organometallic Compounds; D011522:Protons; D011107:Polylysine; D012710:Serum Albumin, Bovine; D004369:Pentetic Acid; D019786:Gadolinium DTPA