Polyclonal and monoclonal antibodies monospecific to MMTV LTR orf protein produced in E. coli.
Monoclonal and polyclonal antibodies specific to an open reading frame of the mouse mammary tumor virus long terminal repeat were generated using an open reading frame-beta-galactosidase fusion protein produced in E. coli. Both antibodies reacted with the open reading frame-beta-galactosidase fusion protein but not with beta-galactosidase alone using an immunoblotting technique. It is concluded that these antibodies were specific for the protein encoded by the open reading frame of the mouse mammary tumor virus long terminal repeat.
Haga
S
S
Second Department of Anatomy, Nara Medical University, Japan.
Shigesada
K
K
Namba
Y
Y
Tanaka
H
H
Imai
S
S
Morimoto
J
J
Hiroishi
S
S
Yamamoto
H
H
Sarkar
N H
NH
Hilgers
J
J
D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D000914:Antibodies, Viral / Q000096:biosynthesis*; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D006046:Gold; D007150:Immunohistochemistry; D008324:Mammary Tumor Virus, Mouse / Q000201:enzymology / Q000235:genetics*; D015514:Oncogene Proteins, Fusion / Q000235:genetics* / Q000276:immunology* / Q000302:isolation & purification; D016366:Open Reading Frames; D010957:Plasmids; D012091:Repetitive Sequences, Nucleic Acid; D001616:beta-Galactosidase / Q000235:genetics* / Q000276:immunology
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D015514:Oncogene Proteins, Fusion; D006046:Gold; D001616:beta-Galactosidase