title: Effect of adenosine triphosphate on the postischemic left ventricular function of the immature myocardium.
abstract: In this study, the effect of exogenous adenosine triphosphate (ATP) on the immature myocardium was evaluated. Isolated working neonatal rabbit hearts were perfused aerobically for 15 min with Krebs-Henseleit buffer (KHB) at 37 degrees C, and then arrested with St. Thomas solution (STS) in group 1 and STS containing 500 mumol/L of ATP in group 2 at 4 degrees to 6 degrees C and maintained at 10 degrees to 14 degrees C for 60 min. Hearts were reperfused with KHB aerobically at 37 degrees C for 15 min. Each heart served as its own control before and after arrest. Systolic function was significantly depressed in group 1 compared with group 2. There was a significant decrease in the peak left ventricular (LV) systolic pressure in group 1 (preischemia mean [PIM] 54 mm Hg to postischemia mean [PoIM] 42 mm Hg, Student's t test p = 0.007) than in group 2 (PIM 66 to PoIM 62 mm Hg, p = 0.5). The LV pulse pressure decreased in group 1 (PIM 72 to PoIM 54 mm Hg, p = 0.02) but not in group 2 (PIM 84 to PoIM 86 mm Hg, p = 0.9) and the rate of rise of LV pressure (dP/dT) in group 2 improved (PIM 5718 to PoIM 6926 mm Hg, p = 0.4) compared with group 1 (PIM 7021 to PoIM 4125 mm Hg, p = 0.008). The PoIM LV flow (LVF) was greater in group 2 than group 1 (LVF group 1 = 2.7 ml/min, group 2 = 4.5 ml/min). Diastolic pressures were not significantly different in the two groups. Our findings suggest that the incorporation of ATP in STS has a significant effect in improving postischemic LV systolic function in neonatal rabbit hearts.
authors.affiliation: Deborah Research Institute, Browns Mills, NJ 08015-1799.
authors.forename: S
authors.initials: S
authors.lastname: Muralidharan
authors.forename: H
authors.initials: H
authors.lastname: Rambaran
authors.forename: G W
authors.initials: GW
authors.lastname: Laub
authors.forename: C
authors.initials: C
authors.lastname: Chen
authors.forename: J
authors.initials: J
authors.lastname: Gu
authors.forename: L B
authors.initials: LB
authors.lastname: McGrath
mesh_terms: D000255:Adenosine Triphosphate / Q000494:pharmacology*; D000818:Animals; D003327:Coronary Disease / Q000503:physiopathology*; D006321:Heart / Q000187:drug effects* / Q000254:growth & development; D006324:Heart Arrest, Induced; D006439:Hemodynamics / Q000187:drug effects / Q000502:physiology; D066298:In Vitro Techniques; D015425:Myocardial Reperfusion / Q000379:methods; D010477:Perfusion / Q000379:methods; D011817:Rabbits; D016277:Ventricular Function, Left / Q000187:drug effects* / Q000502:physiology
chemical_list: D000255:Adenosine Triphosphate