[Detection of DNA-binding proteins in polyacrylamide gel after denaturing electrophoresis].
A simple method for detection of DNA-binding proteins is offered. These proteins can be revealed, following their electrophoretic separation in sodium dodecyl sulfate (SDS)-polyacrylamide gel containing labeled DNA, by washing the gel in buffer to remove SDS and to allow protein renaturation. Protein-free DNA is washed out, remaining in the DNA-binding proteins that restored their original characteristic. After autoradiography these proteins are seen as black bands (by one-dimensional gel electrophoresis) or spots (by two-dimensional gel electrophoresis) on a grey background. High sensitivity of the method is shown by using protein fractions of rat liver and a standard method.
Naryzhnyĭ
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SN
D000818:Animals; D001345:Autoradiography; D015153:Blotting, Western; D004247:DNA / Q000737:chemistry; D004268:DNA-Binding Proteins / Q000032:analysis*; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D008099:Liver / Q000737:chemistry; D008297:Male; D011489:Protein Denaturation; D051381:Rats
D004268:DNA-Binding Proteins; D004247:DNA
Obnaruzhenie DNK-sviazyvaiushchikh belkov v poliakrilamidnom gele posle denaturiruiushchego élektroforeza.