title: Biosynthesis of griseolic acids: incorporation of 13C-labeled compounds into griseolic acid A.
abstract: Biosynthesis of griseolic acids, competitive inhibitors of cyclic nucleotide phosphodiesterase, was investigated with the culture of a producing strain of Streptomyces griseoaurantiacus. 13C-Labeled and 15N-labeled compounds were added into the culture, and 13C-enriched and 15N-enriched griseolic acid A was isolated from the culture medium and analyzed by 13C NMR and 15N NMR spectroscopy. The compounds added to growth medium were [2-13C]acetate, [1,2-13C]acetate, [1,4-13C]succinate, [1-13C]glucose, [6-13C]glucose, [2-13C]ribose, and [1-13C, 15N]glycine. The results suggest that adenosine, which is formed from amino acids and sugars contributes the adenine and ribose moieties to griseolic acid A. The data also suggest that a dicarboxylic acid from the Krebs tricarboxylic acid cycle contributes to the dicarboxylic part of the compound.
authors.affiliation: Fermentation Research Laboratories, Sankyo Co., Ltd., Tokyo, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Miyakoshi
authors.forename: H
authors.initials: H
authors.lastname: Haruyama
authors.forename: T
authors.initials: T
authors.lastname: Shioiri
authors.forename: S
authors.initials: S
authors.lastname: Takahashi
authors.forename: A
authors.initials: A
authors.lastname: Torikata
authors.forename: M
authors.initials: M
authors.lastname: Yamazaki
mesh_terms: D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000037:antagonists & inhibitors*; D000241:Adenosine / Q000031:analogs & derivatives* / Q000096:biosynthesis; D002247:Carbon Isotopes; D013302:Streptomyces / Q000378:metabolism
chemical_list: D002247:Carbon Isotopes; C046499:griseolic acid; D015105:3',5'-Cyclic-AMP Phosphodiesterases; D000241:Adenosine