Historical progress and the future of human cell culture research.
Progress in human cell culture research is discussed based primarily on our hematopoietic cell culture studies. The article includes a historical background of Burkitt lymphoma cell lines, discovery of EBV, normal B-lymphoblastoid cell lines with EBV, a variety of leukemia, lymphoma, and myeloma cell lines, clinical and theoretical contributions made by studies of T-cell leukemia cell lines, the discovery and clinical relevance of HTLV, HIV and HBLV, early attempts at adoptive immunotherapy of patients with cancer, and the future of human cell culture research. Despite the fact that current cell culture methods permit maintenance of only limited cell types of both normal and malignant origins, biotechnological advances such as hybridoma and recombinant DNA technologies should continue to provide unlimited research opportunities in all fields.
Moore
G E
GE
University of Colorado Medical Center, Division of Surgical Oncology, Denver General Hospital.
Minowada
J
J
D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology; D002051:Burkitt Lymphoma / Q000473:pathology*; D003585:Cell Biology / Q000639:trends; D002455:Cell Division; D002478:Cells, Cultured; D006224:Cricetinae; D003470:Culture Media; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D004854:Herpesvirus 4, Human; D015368:Human T-lymphotropic virus 1; D015367:Human T-lymphotropic virus 2; D006801:Humans; D016219:Immunotherapy, Adoptive; D007938:Leukemia / Q000473:pathology; D008223:Lymphoma / Q000473:pathology / Q000628:therapy; D051379:Mice; D051381:Rats; D014407:Tumor Cells, Cultured
D003470:Culture Media