Isolation and characterization of two sub-species of Lp(a), one containing apo E and one free of apo E.
Lipoprotein Lp(a) was isolated by immunoaffinity chromatography using anti apolipoprotein B and anti apolipoprotein (a) immunosorbents. Besides apolipoproteins (a) and B, this fraction was shown to contain apolipoproteins C and E. Therefore, it was decided to further purify this crude Lp(a) into particles containing apolipoprotein E and particles free of apo E, using chromatography with an anti apolipoprotein E immunosorbent. Lp(a), free of apolipoprotein E was cholesterol ester rich and triacylglycerol poor and was found mainly in the LDL size range. In contrast, Lp(a) containing apolipoprotein E was triacylglycerol rich and was distributed mainly in the VLDL and IDL size range. Binding of these two fractions, one containing apo E and one free of it, to the apo B/E receptor of HeLa cells was studied. Both fractions bound to the receptor but the one containing apo E had a better affinity than the one free of apo E. Further studies are needed to identify the clinical importance of these two different entities.
Bard
J M
JM
SERLIA, Institut Pasteur, Lille, France.
Delattre-Lestavel
S
S
Clavey
V
V
Pont
P
P
Derudas
B
B
Parra
H J
HJ
Fruchart
J C
JC
D001053:Apolipoproteins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D001057:Apolipoproteins E / Q000302:isolation & purification*; D053241:Apoprotein(a); D002846:Chromatography, Affinity; D006367:HeLa Cells / Q000378:metabolism; D006801:Humans; D008055:Lipids / Q000097:blood; D017270:Lipoprotein(a); D008074:Lipoproteins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D010316:Particle Size; D011956:Receptors, Cell Surface / Q000378:metabolism; D018110:Receptors, Lipoprotein
D001053:Apolipoproteins; D001057:Apolipoproteins E; D008055:Lipids; D017270:Lipoprotein(a); D008074:Lipoproteins; D011956:Receptors, Cell Surface; D018110:Receptors, Lipoprotein; C032531:apolipoprotein B,E receptor; D053241:Apoprotein(a)