Prolactin-like substance secretion by granulosa cells isolated from bovine ovaries.
By the 5-day culture of bovine granulosa cells both in serum-free and in serum-supplemented medium the time-dependent accumulation of PRL immunoreactivity was observed. FSH additions (10-10,000 ng/ml medium) led to a dramatic rise of immunoreactive PRL in a dose-dependent manner. LH stimulated the increase of PRL-like substance only in a great dose (10 IU/ml). Lower LH doses (0.01-1 IU/ml) had no significant influence on this process. Low doses of oxytocin (1 or 10 mIU/ml) blocked, and higher ones (100 or 1,000 mIU/ml) stimulated the granulosa PRL-like substance accumulation. Arginine-8-vasopressin (1-1,000 ng/ml), arginine-8-vasotocin (10-10,000 ng/ml), or LH-RH (10-10,000 ng/ml) failed to influence the PRL immunoreactivity accumulation in the culture medium. Present data may suggest the production of PRL-like substance by bovine ovarian cells, as well as the involvement of gonadotropins and oxytocin in the regulation of this process.
Sirotkin
A V
AV
Research Institute of Animal Production, Nitra, Slovakia.
Nitray
J
J
Politov
V P
VP
Marinchenko
P
P
D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005260:Female; D005640:Follicle Stimulating Hormone / Q000008:administration & dosage / Q000494:pharmacology; D006107:Granulosa Cells / Q000378:metabolism*; D066298:In Vitro Techniques; D007986:Luteinizing Hormone / Q000008:administration & dosage / Q000494:pharmacology; D010053:Ovary / Q000166:cytology*; D010121:Oxytocin / Q000008:administration & dosage / Q000494:pharmacology; D011388:Prolactin / Q000276:immunology / Q000378:metabolism*
D010121:Oxytocin; D011388:Prolactin; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone