title: Comparison of an EBV transformed cell line and an EBV hybridoma cell line producing the same human anti-HBs monoclonal antibody.
abstract: A stable human-mouse heterohybridoma secreting human anti-HBs monoclonal antibody in continuous culture for 12 months was generated. It grew faster than the parent EBV transformed lymphoblastoid cell line (LCL) but produced the same level of specific antibody. The LCL was positive for the Epstein-Barr Virus Nuclear Antigen (EBNA), human CD 23 and contained a diploid number of human chromosomes. The heterohybridoma was negative for EBNA, CD 23 and mouse Ly-1 mouse, despite retaining a full complement of diploid mouse chromosomes and a limited number of human chromosomes.
authors.affiliation: Department of Clinical Sciences, London School of Hygiene and Tropical Medicine, UK.
authors.forename: A
authors.initials: A
authors.lastname: Sa'adu
authors.forename: H
authors.initials: H
authors.lastname: Walker
authors.forename: M
authors.initials: M
authors.lastname: Locniskar
authors.forename: D
authors.initials: D
authors.lastname: Bidwell
authors.forename: C
authors.initials: C
authors.lastname: Howard
authors.forename: K P
authors.initials: KP
authors.lastname: McAdam
authors.forename: A
authors.initials: A
authors.lastname: Voller
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology; D001667:Binding, Competitive; D015415:Biomarkers; D002455:Cell Division; D002461:Cell Line, Transformed / Q000276:immunology*; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D006510:Hepatitis B Antibodies / Q000096:biosynthesis / Q000276:immunology; D006514:Hepatitis B Surface Antigens / Q000276:immunology; D004854:Herpesvirus 4, Human; D006801:Humans; D006825:Hybridomas / Q000276:immunology*; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000276:immunology; D051379:Mice
chemical_list: D000911:Antibodies, Monoclonal; D015415:Biomarkers; D006510:Hepatitis B Antibodies; D006514:Hepatitis B Surface Antigens; D007074:Immunoglobulin G; D004247:DNA