title: Comparison of virus isolation, immunofluorescence and DNA probe hybridization for detection of pseudorabies virus in experimentally infected pigs.
abstract: Virus isolation, immunofluorescent staining and DNA probe hybridization, three techniques for the detection of pseudorabies virus (PRV) have been compared in pigs experimentally infected with the Thailand CB-1 strain PRV. The virus isolation and DNA hybridization detection demonstrated a good correlation in detecting infection in live animals by nasal swabbing. In white blood cells an earlier detection was seen with the DNA-hybridization techniques. Consistent results with all the three techniques tested were found in organ materials as nasal mucosa and tonsils as well as in the olfactory bulb.
authors.affiliation: Department of Pathology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand.
authors.forename: R
authors.initials: R
authors.lastname: Wongwatcharadumrong
authors.forename: A
authors.initials: A
authors.lastname: Kunavongkrit
authors.forename: M
authors.initials: M
authors.lastname: Neumüller
authors.forename: T
authors.initials: T
authors.lastname: Linné
mesh_terms: D000818:Animals; D015342:DNA Probes; D005455:Fluorescent Antibody Technique; D011558:Herpesvirus 1, Suid / Q000302:isolation & purification*; D009693:Nucleic Acid Hybridization; D011557:Pseudorabies / Q000382:microbiology*; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology*
chemical_list: D015342:DNA Probes