Conjugal transfer of plasmid and transposon DNA from Escherichia coli into Porphyromonas gingivalis.
Using the broad host-range vector R751 to provide transfer functions, plasmid pVAL-1 and transposon Tn4351 were conjugally mobilized from Escherichia coli into Porphyromonas gingivalis. Transfer frequencies for both elements varied between 10(-6) and 10(-11), depending upon the recipient. The behavior of pVAL-1 and Tn4351 in P. gingivalis was essentially as described previously in Bacteroides spp. These data indicate that plasmid and transposon DNA can be conjugally transferred into P. gingivalis and that these elements can be used to genetically manipulate the organism in examining putative virulence determinants that may participate in the induction or exacerbation of periodontal disease.
Dyer
D W
DW
Department of Microbiology, School of Medicine and Biomedical Sciences, State University of New York, Buffalo 14214.
Bilalis
G
G
Michel
J H
JH
Malek
R
R
D001345:Autoradiography; D015139:Blotting, Southern; D003227:Conjugation, Genetic; D004261:DNA Replication; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics*; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D010761:Phosphorus Radioisotopes; D010957:Plasmids; D016966:Porphyromonas gingivalis / Q000235:genetics*
D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D010761:Phosphorus Radioisotopes