title: A high-molecular-weight (greater than 8.10(5)) non-collagenous glycoprotein is synthesized by bovine cartilage in vitro.
abstract: A high-molecular-weight (greater than 8.10(5)) glycoprotein was detected in [3H]glucosamine-labeled bovine cartilage. Extraction with varying amounts of guanidinium chloride showed that the molecule was not tightly bound to other matrix substances. Enzyme digestions identified the molecule as a non-collagenous glycoprotein. This glycoprotein constituted 10-20% of the [3H]glucosamine-labeled macromolecular material that was released into culture medium on the first day after labeling. The 3H-labeled glycoprotein was purified by anion-exchange chromatography, CsCl gradient centrifugation and gel filtration. The purified glycoprotein appeared on an SDS-polyacrylamide gel as one slightly polydisperse band, which could not be reduced by beta-mercaptoethanol.
authors.affiliation: Jan van Breemen Institute for Rheumatology and Rehabilitation, Amsterdam, The Netherlands.
authors.forename: N A
authors.initials: NA
authors.lastname: Visser
authors.forename: H S
authors.initials: HS
authors.lastname: Brand
authors.forename: G P
authors.initials: GP
authors.lastname: Vankampen
authors.forename: R J
authors.initials: RJ
authors.lastname: Vandestadt
authors.forename: J K
authors.initials: JK
authors.lastname: Vanderkorst
mesh_terms: D000818:Animals; D002358:Cartilage, Articular / Q000737:chemistry / Q000378:metabolism*; D002417:Cattle; D002478:Cells, Cultured; D005260:Female; D006023:Glycoproteins / Q000096:biosynthesis* / Q000302:isolation & purification; D006821:Hyaluronoglucosaminidase; D006868:Hydrolysis; D003012:Microbial Collagenase; D008970:Molecular Weight; D014316:Tritium
chemical_list: D006023:Glycoproteins; D014316:Tritium; D006821:Hyaluronoglucosaminidase; D003012:Microbial Collagenase