title: The influence of alpha- and beta-galactose residues and sialic acid O-acetyl groups of rat erythrocytes on the interaction with peritoneal macrophages.
abstract: The significance of glycoconjugates on the surface of rat erythrocytes was studied in the interaction of these cells with homologous peritoneal macrophages. The erythrocytes exposing terminal alpha-galactose and thus of B blood group specificity, as well as sialic acid are not bound by the macrophages. beta-Galactose residues exposed by sialidase induced strong binding and additional alpha-galactosidase treatment enhanced the binding. beta-Galactose exposed on glycolipids after pronase and alpha-galactosidase treatment induced no binding. An intact protein core of the glycoproteins on the erythrocyte surface was necessary for interaction with macrophages. Partial de-O-acetylation of sialic acids prior to sialidase treatment stimulated subsequent binding of the erythrocytes.
authors.affiliation: Biochemisches Institut, Universität zu Kiel.
authors.forename: K
authors.initials: K
authors.lastname: Kiehne
authors.forename: R
authors.initials: R
authors.lastname: Schauer
mesh_terms: D000107:Acetylation; D000818:Animals; D002448:Cell Adhesion; D002450:Cell Communication; D004912:Erythrocytes / Q000737:chemistry* / Q000201:enzymology / Q000502:physiology; D005690:Galactose / Q000737:chemistry / Q000502:physiology*; D008264:Macrophages / Q000737:chemistry* / Q000502:physiology; D010529:Peritoneal Cavity / Q000166:cytology; D051381:Rats; D012794:Sialic Acids / Q000097:blood* / Q000737:chemistry; D013329:Structure-Activity Relationship; D000519:alpha-Galactosidase / Q000737:chemistry
chemical_list: D012794:Sialic Acids; D000519:alpha-Galactosidase; D005690:Galactose