Herpesvirus vector gene transfer and expression of beta-glucuronidase in the central nervous system of MPS VII mice.
Genetic disorders affecting the central nervous system (CNS) can potentially be treated by gene transfer using vectors which infect and express genes in post-mitotic neurons. Herpesviruses establish latent infections in neurons during which only one viral gene (LAT) is expressed, thus the LAT promoter may express foreign genes in latently infected CNS cells. Expression of a beta-glucuronidase gene driven by the LAT promoter was tested in mice lacking this enzyme, which are a model for a human genetic disease affecting the CNS (mucopolysaccharidosis VII, Sly disease). Cells expressing the missing enzymatic activity were present in the trigeminal ganglia and brainstems of latently infected animals, up to four months post-inoculation, demonstrating the potential of this approach for the long-term expression of foreign genes in the CNS.
Wolfe
J H
JH
School of Veterinary Medicine, University of Pennsylvania, Philadelphia 19104.
Deshmane
S L
SL
Fraser
N W
NW
D000818:Animals; D001933:Brain Stem / Q000378:metabolism*; D005814:Genes, Viral; D005822:Genetic Vectors; D005966:Glucuronidase / Q000235:genetics* / Q000378:metabolism*; D017403:In Situ Hybridization; D051379:Mice; D008815:Mice, Inbred Strains; D009474:Neurons / Q000378:metabolism; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D015183:Restriction Mapping; D018139:Simplexvirus / Q000235:genetics*; D014162:Transfection / Q000379:methods*; D012668:Trigeminal Ganglion / Q000378:metabolism*
D011993:Recombinant Fusion Proteins; D005966:Glucuronidase