Inhibition of prostaglandin delta 13 reductase activity in rabbit kidney cortex by glutathione disulfide.
t-Butyl hydroperoxide and H2O2-Fe(2+)-EDTA-glutathione system which produces hydroxyl radicals did not affect the 15-hydroxy prostaglandin dehydrogenase activity in rabbit kidney cortex. On the other hand, H2O2-Fe(2+)-EDTA-glutathione system inhibited the prostaglandin delta 13 reductase activity. Mannitol, a scavenger of hydroxyl radicals, had no effect on the inhibitory action of this system, indicating that the effect of H2O2-Fe(2+)-EDTA-glutathione system on the prostaglandin delta 13 reductase may not be due to produced hydroxyl radicals. As a result of further investigation, it was shown that glutathione disulfide, which is synthesized concomitantly with hydroxyl radicals from H2O2-Fe(2+)-EDTA-glutathione, inhibited the prostaglandin delta 13 reductase activity. These results suggest that hydroperoxides and hydroxyl radicals may not be likely candidates for the modulator of the catabolism of prostaglandins in the kidney cortex, and that glutathione disulfide has the potential to modulate the prostaglandin catabolism by affecting the prostaglandin delta 13 reductase activity.
Sakuma
S
S
Department of Hygienic Chemistry, Osaka University of Pharmaceutical Sciences, Japan.
Fujimoto
Y
Y
Nishida
H
H
Sumiya
T
T
Yamamoto
I
I
Fujita
T
T
D011452:15-Oxoprostaglandin 13-Reductase / Q000037:antagonists & inhibitors*; D000818:Animals; D015232:Dinoprostone / Q000378:metabolism; D004492:Edetic Acid / Q000494:pharmacology; D005296:Ferrous Compounds / Q000494:pharmacology; D005978:Glutathione / Q000031:analogs & derivatives* / Q000494:pharmacology; D019803:Glutathione Disulfide; D006911:Hydroxyprostaglandin Dehydrogenases / Q000187:drug effects; D066298:In Vitro Techniques; D007672:Kidney Cortex / Q000378:metabolism*; D008297:Male; D010545:Peroxides / Q000494:pharmacology; D011817:Rabbits; D020122:tert-Butylhydroperoxide
D005296:Ferrous Compounds; D010545:Peroxides; C055526:Fe(II)-EDTA; D020122:tert-Butylhydroperoxide; D004492:Edetic Acid; D006911:Hydroxyprostaglandin Dehydrogenases; C035859:15-hydroxyprostaglandin dehydrogenase; D011452:15-Oxoprostaglandin 13-Reductase; D005978:Glutathione; D015232:Dinoprostone; D019803:Glutathione Disulfide