title: Biochemical properties and subcellular distribution of an N-type calcium channel alpha 1 subunit.
abstract: A site-directed anti-peptide antibody, CNB-1, that recognizes the alpha 1 subunit of rat brain class B calcium channels (rbB) immunoprecipitated 43% of the N-type calcium channels labeled by [125I]omega-conotoxin. CNB-1 recognized proteins of 240 and 210 kd, suggesting the presence of two size forms of this alpha 1 subunit. Calcium channels recognized by CNB-1 were localized predominantly in dendrites; both dendritic shafts and punctate synaptic structures upon the dendrites were labeled. The large terminals of the mossy fibers of the dentate gyrus granule neurons were heavily labeled, suggesting that the punctate labeling pattern represents calcium channels in nerve terminals. The pattern of immunostaining was cell specific. The cell bodies of some pyramidal cells in layers II, III, and V of the dorsal cortex, Purkinje cells, and scattered cell bodies elsewhere in the brain were also labeled at a low level. The results define complementary distributions of N- and L-type calcium channels in dendrites, nerve terminals, and cell bodies of most central neurons and support distinct functional roles in calcium-dependent electrical activity, intracellular calcium regulation, and neurotransmitter release for these two channel types.
authors.affiliation: Department of Pharmacology, University of Washington, Seattle 98195.
authors.forename: R E
authors.initials: RE
authors.lastname: Westenbroek
authors.forename: J W
authors.initials: JW
authors.lastname: Hell
authors.forename: C
authors.initials: C
authors.lastname: Warner
authors.forename: S J
authors.initials: SJ
authors.lastname: Dubel
authors.forename: T P
authors.initials: TP
authors.lastname: Snutch
authors.forename: W A
authors.initials: WA
authors.lastname: Catterall
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies; D000911:Antibodies, Monoclonal; D001921:Brain / Q000166:cytology / Q000378:metabolism*; D015220:Calcium Channels / Q000378:metabolism*; D002540:Cerebral Cortex / Q000166:cytology / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D006624:Hippocampus / Q000166:cytology / Q000378:metabolism; D015151:Immunoblotting; D007150:Immunohistochemistry; D046911:Macromolecular Substances; D008636:Mesencephalon / Q000166:cytology / Q000378:metabolism; D008969:Molecular Sequence Data; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology; D010456:Peptides, Cyclic / Q000378:metabolism; D016548:Prosencephalon / Q000166:cytology / Q000378:metabolism; D011689:Purkinje Cells / Q000166:cytology / Q000378:metabolism; D011712:Pyramidal Tracts / Q000166:cytology / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D017981:Receptors, Neurotransmitter / Q000378:metabolism*; D013569:Synapses / Q000502:physiology / Q000648:ultrastructure; D020960:omega-Conotoxins
chemical_list: D000906:Antibodies; D000911:Antibodies, Monoclonal; D015220:Calcium Channels; D046911:Macromolecular Substances; D010455:Peptides; D010456:Peptides, Cyclic; D017981:Receptors, Neurotransmitter; D020960:omega-Conotoxins; C053157:omega-conotoxin receptor; C053423:Conus magus toxin