title: Isolation and purification of calmodulin from the shrimp, Crangon crangon.
abstract: Calmodulin was isolated and purified from shrimp abdominal muscle by heat precipitation, ion exchange and hydrophobic interaction chromatography. The purified calmodulin was homogeneous when evaluated by polyacrylamide gel electrophoresis. A still remaining contaminant was eliminated by high performance liquid chromatography on a phenyl column. The biological and physicochemical properties of shrimp calmodulin such as amino acid composition, molecular weight and the ability to activate calmodulin-deficient bovine heart phosphodiesterase were compared to those of other invertebrate calmodulins.
authors.affiliation: Universität Oldenburg, Dept. Zoophysiology, Germany.
authors.forename: R H
authors.initials: RH
authors.lastname: Michael
authors.forename: R
authors.initials: R
authors.lastname: Pipkorn
authors.forename: A
authors.initials: A
authors.lastname: Willig
authors.forename: P P
authors.initials: PP
authors.lastname: Jaros
mesh_terms: D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000032:analysis; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002147:Calmodulin / Q000737:chemistry / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D033364:Decapoda / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D004789:Enzyme Activation; D008970:Molecular Weight
chemical_list: D000596:Amino Acids; D002147:Calmodulin; D015105:3',5'-Cyclic-AMP Phosphodiesterases