title: Cell-specific envelope glycosylation distinguishes FIV glycoproteins produced in cytopathically and noncytopathically infected cells.
abstract: Feline immunodeficiency virus (FIV) infection induces syncytium formation and cell death in primary feline astrocyte cultures but persistently and noncytopathically infects Crandell feline kidney cells (CrFK). Because viral envelope glycoproteins are implicated in cell fusion events we evaluated the astrocyte-produced FIV surface glycoprotein for properties that might distinguish it from that produced in CrFK cells. The surface glycoprotein from astrocytes migrated faster on SDS-PAGE and contained more Endo H-sensitive oligosaccharides than that from CrFK, although the precursor and deglycosylated envelope glycoproteins from both cells were the same size. Castanospermine treatment of infected astrocytes, which blocks glucose trimming from oligosaccharide side chains of glycoproteins, both obliterated the mobility difference between astrocyte- and CrFK-produced FIV surface glycoproteins and prevented syncytium in infected astrocyte cultures. These results demonstrate the importance of the infected cell type in viral envelope protein glycosylation and implicate cell type-specific carbohydrate structures on retroviral glycoproteins as mediators of cell fusion.
authors.affiliation: Department of Pathology, Colorado State University, Fort Collins 80523.
authors.forename: M L
authors.initials: ML
authors.lastname: Poss
authors.forename: S W
authors.initials: SW
authors.lastname: Dow
authors.forename: E A
authors.initials: EA
authors.lastname: Hoover
mesh_terms: D001253:Astrocytes / Q000382:microbiology; D016923:Cell Death; D002460:Cell Line; D003588:Cytopathogenic Effect, Viral; D004797:Enzyme-Linked Immunosorbent Assay; D015726:Giant Cells / Q000166:cytology; D006026:Glycoside Hydrolases / Q000037:antagonists & inhibitors / Q000378:metabolism; D006031:Glycosylation; D016089:Immunodeficiency Virus, Feline / Q000378:metabolism* / Q000502:physiology; D007212:Indolizines / Q000494:pharmacology; D007700:Kinetics; D008562:Membrane Glycoproteins / Q000378:metabolism*; D015531:Radioimmunoprecipitation Assay; D014759:Viral Envelope Proteins / Q000378:metabolism*; D014779:Virus Replication; D054836:Indolizidines
chemical_list: D006026:Glycoside Hydrolases; D007212:Indolizines; D008562:Membrane Glycoproteins; D014759:Viral Envelope Proteins; C037806:castanospermine; D054836:Indolizidines