Phospholipids in Trypanosoma cruzi: phosphoinositide composition and turnover.
The polyphosphoinositides from Trypanosoma cruzi were isolated by preparative thin-layer chromatography (TLC) and identified. When myo-[3H]inositol was present in the culture medium for five days, analyses showed the presence of phosphatidylinositol (PI), lysophosphatidylinositol (lysoPI), phosphatidylinositol 4-phosphate (PIP) and phosphatidylinositol 4,5-bisphosphate (PIP2). Short-term incubation with 32Pi led to higher percentages of incorporation into phosphatidylethanolamine (PE), lysophosphatidylethanolamine (lysoPE) and PI compared to the other glycerophospholipids. The phosphoinositides (PI, PIP and PIP2) contained a larger proportion of unsaturated than saturated fatty acids. High proportions of 18:2 were found in the three phosphoinositides analyzed, whereas the major saturated fatty acid was 18:0. Water-soluble inositol phosphates (IP, IP2 and IP3) were also identified.
Racagni
G
G
Departamento de Biología Molecular, Facultad de Ciencias Exactas, Universidad Nacional de Río Cuarto, Córdoba, Argentina.
García de Lema
M
M
Domenech
C E
CE
Machado de Domenech
E E
EE
D000818:Animals; D001711:Biotransformation; D002849:Chromatography, Gas; D002855:Chromatography, Thin Layer; D010716:Phosphatidylinositols / Q000032:analysis / Q000493:pharmacokinetics*; D010761:Phosphorus Radioisotopes; D014316:Tritium; D014349:Trypanosoma cruzi / Q000378:metabolism*
D010716:Phosphatidylinositols; D010761:Phosphorus Radioisotopes; D014316:Tritium