A low cost method to produce a gaseous environment for the isolation of Helicobacter pylori.
A low cost method (LCM) to produce a gaseous environment for the isolation of Helicobacter pylori, was compared with the standard Gas Park system. The LCM uses a carbonated antacid tablet, a plastic bag with tap water, a candle, and a wide-mouthed glass jar provided with a tight-fitting metallic screw cap and a rubber gasket. Antral gastric biopsies from 153 cases were incubated by duplicate on blood agar plates and treated with the two methods. In 95 cases the agent was isolated from both, and only from the standard method in 10 cases; the opposite condition was found in five cases, and 43 were negative. That difference is not significant (Pearson's chi 2 = 93.25 p > 0.05).
Hernández
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F
Departamento de Microbiología, Facultad de Microbiología, Universidad de Costa Rica, San José.
Rivera
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P
D001241:Aspirin; D001431:Bacteriological Techniques; D002951:Citrates; D003470:Culture Media; D004338:Drug Combinations; D016480:Helicobacter pylori / Q000302:isolation & purification*; D006801:Humans; D011706:Pyloric Antrum / Q000382:microbiology; D017693:Sodium Bicarbonate
D002951:Citrates; D003470:Culture Media; D004338:Drug Combinations; C002931:sodium acetylsalicylate, sodium bicarbonate, sodium citrate drug combination; D017693:Sodium Bicarbonate; D001241:Aspirin