Highly sensitive enhanced chemiluminescence immunodetection method for herpes simplex virus type 2 Western immunoblot.
Three Western blot (immunoblot) methods for detecting antibodies to herpes simplex virus type 2 were compared: (i) nitrocellulose blots with 4-chloro-1-naphthol immunostaining (4CN-WB); (ii) polyvinylidene difluoride (PVDF) blots with 3,3',5,5'-tetramethylbenzidene immunostaining (TMB-WB); and (iii) PVDF blots with enhanced chemiluminescence (ECL-WB). TMB-WB was 10-fold more sensitive than 4CN-WB, while ECL-WB was as much as 500-fold more sensitive.
Dalessio
J
J
Department of Laboratory Medicine, University of Washington, Seattle 98195.
Ashley
R
R
D000914:Antibodies, Viral / Q000097:blood*; D015153:Blotting, Western / Q000379:methods* / Q000706:statistics & numerical data; D005069:Evaluation Studies as Topic; D006558:Herpes Genitalis / Q000175:diagnosis / Q000276:immunology; D006801:Humans; D008163:Luminescent Measurements; D012680:Sensitivity and Specificity; D018139:Simplexvirus / Q000276:immunology*
D000914:Antibodies, Viral