Additive enhancement of neutrophil collagenase activity by HOCl and cathepsin G.
Using preparations of latent collagenase derived from neutrophil specific granule extracts, the relative effects of cathepsin G and HOCl on activation of neutrophil collagenase were determined using a quantitative collagenase assay. Enhancement of collagenase activity by cathepsin G and physiologically relevant concentrations of HOCl were comparable, but HOCl mediated collagenase activation was reversible in the presence of HOCl scavenger. Collagenase activity in preparations treated with cathepsin G and HOCL simultaneously or sequentially was significantly greater than activity in preparations treated with HOCl or cathepsin G alone. The results indicated additive, yet independent enhancing effects of HOCl and cathepsin G on activity of neutrophil collagenase.
Chatham
W W
WW
Department of Medicine, University of Alabama, Birmingham.
Blackburn
W D
WD
Heck
L W
LW
D056649:Cathepsin G; D002403:Cathepsins / Q000097:blood* / Q000302:isolation & purification / Q000494:pharmacology; D004347:Drug Interactions; D004789:Enzyme Activation; D006801:Humans; D007700:Kinetics; D008634:Mersalyl / Q000494:pharmacology; D003012:Microbial Collagenase / Q000097:blood*; D009504:Neutrophils / Q000201:enzymology*; D012697:Serine Endopeptidases; D012973:Sodium Hypochlorite / Q000494:pharmacology*
D008634:Mersalyl; D012973:Sodium Hypochlorite; D002403:Cathepsins; D012697:Serine Endopeptidases; C534094:CTSG protein, human; D056649:Cathepsin G; D003012:Microbial Collagenase