Effectiveness of chemical agents in removing platinum from DNA isolated from cisplatin-treated HL-60 cells.
Human promyelocytic leukemia cells, HL-60, were treated with cisplatin [cis-diamminedichloroplatinum (II)] (2 mM, 1 h). DNA-cisplatin-protein complexes were isolated and exposed to thiourea (1 M), NaCN (100 mM), diethyldithio-carbamate (500 mM), or N-methyl-D-glucamine dithiocarbamate (500 mM) for 12 h. The release of platinum was measured by atomic absorption spectroscopy. Sodium cyanide was the most effective agent, releasing about 90% of the DNA-bound platinum. Thiourea was the least effective agent, while dithiocarbamates exhibited an intermediate. The ability of the same group of agents to split the proteins off from the protein-cisplatin-DNA complex was also evaluated and similarly dithiocarbamate were also the most effective.
Oliński
R
R
Department of Clinical Biochemistry, Medical School, Bydgoszcz, Poland.
Briggs
R C
RC
Basinger
M
M
Jones
M M
MM
D002614:Chelating Agents; D002945:Cisplatin / Q000302:isolation & purification* / Q000378:metabolism*; D004247:DNA / Q000302:isolation & purification*; D018736:DNA Adducts; D004273:DNA, Neoplasm / Q000302:isolation & purification*; D004050:Ditiocarb; D006801:Humans; D007202:Indicators and Reagents; D015473:Leukemia, Promyelocytic, Acute; D008623:Mercaptoethanol; D009363:Neoplasm Proteins / Q000302:isolation & purification; D012966:Sodium Cyanide; D013012:Sorbitol / Q000031:analogs & derivatives; D013113:Spin Labels; D013859:Thiocarbamates; D013890:Thiourea; D014407:Tumor Cells, Cultured
D002614:Chelating Agents; D018736:DNA Adducts; D004273:DNA, Neoplasm; D007202:Indicators and Reagents; D009363:Neoplasm Proteins; D013113:Spin Labels; D013859:Thiocarbamates; C057270:cisplatin-DNA adduct; D013012:Sorbitol; D008623:Mercaptoethanol; C041262:N-methyl-D-glucamine dithiocarbamate; D004247:DNA; D004050:Ditiocarb; D013890:Thiourea; D012966:Sodium Cyanide; D002945:Cisplatin