Regulation of adrenocorticotropin secretion from cultured canine anterior pituitary cells.
Pituitary cells, collected from five healthy dogs, were cultured and treated with various doses of ovine corticotropin-releasing hormone (CRH), arginine vasopressin (AVP), oxytocin (OT), or angiotensin II (AII) to determine which of these hypothalamic peptides affected adrenocorticotropin (ACTH) secretion. Of the 4 peptides, only CRH significantly increased ACTH secretion from cultured canine anterior pituitary cells. The lowest dose of CRH tested, 0.01 nM, significantly stimulated ACTH release. Co-addition of AVP, OT, or AII with CRH did not increase ACTH secretion beyond that caused by addition of CRH alone. Similarly, neither co-addition of AVP with OT, AVP with AII, or OT with AII significantly stimulated ACTH secretion. These results support a role for CRH in the physiologic regulation of ACTH secretion from the canine anterior pituitary, but do not support regulatory roles for AVP, OT, or AII.
Kemppainen
R J
RJ
Department of Physiology and Pharmacology, Auburn University College of Veterinary Medicine, AL 36849.
Clark
T P
TP
Sartin
J L
JL
Zerbe
C A
CA
D000324:Adrenocorticotropic Hormone / Q000378:metabolism*; D000804:Angiotensin II / Q000502:physiology; D000818:Animals; D001127:Arginine Vasopressin / Q000502:physiology; D002478:Cells, Cultured; D003346:Corticotropin-Releasing Hormone / Q000502:physiology; D004285:Dogs / Q000502:physiology*; D005260:Female; D008297:Male; D009479:Neuropeptides / Q000502:physiology*; D010121:Oxytocin / Q000502:physiology; D010903:Pituitary Gland, Anterior / Q000166:cytology / Q000378:metabolism*
D009479:Neuropeptides; D000804:Angiotensin II; D001127:Arginine Vasopressin; D010121:Oxytocin; D000324:Adrenocorticotropic Hormone; D003346:Corticotropin-Releasing Hormone