The effect of cold storage of rat thoracic aortic rings in organ preservation solutions--a study of receptor-linked vascular prostacyclin synthesis.
An important aspect of organ preservation is the maintenance of intrinsic dilator and antithrombotic mechanisms of blood vessels. Blood vessels synthesize prostacyclin (PGI2), a potent vasodilator and inhibitor of platelet adhesion and aggregation. PGI2 synthesis is controlled by complex mechanisms including adrenoceptor-linked calcium influx and protein kinase C. Since organ preservation solutions may influence these mechanisms, we investigated the effect on in vitro PGI2 synthesis of cold storage of rat aortic rings in lactobionate-raffinose solution (LRS) and hypertonic citrate kidney preservation solution (KPS) on in vitro PGI2 synthesis. Acute incubation of aortic tissue in both preservation solutions at 37 degrees C (compared with minimal essential medium) completely inhibited PGI2 synthesis when stimulated with noradrenaline (NA), phorbol ester (a protein kinase C activator), NaF (a G protein activator), or A23187. Following storage of aortic rings at 4 degrees C (for up to 72 hr) in LRS and KPS, subsequent washing and incubation in MEM, PGI2 synthesis was initially markedly enhanced in response to NA when compared with tissues stored in MEM. These enhanced responses disappeared, and PGI2 synthesis returned to normal following 1 hr incubation of tissues in MEM at 37 degrees C. These data demonstrate that cold storage in preservation fluids exerts minimal deleterious effects, not only on PGI2 synthesis, but possibly on other key processes (calcium homeostasis, protein kinase C activity) in blood vessels.
Jeremy
J Y
JY
Department of Chemical Pathology and Human Metabolism, Royal Free Hospital, England.
Stansby
G
G
Fuller
B
B
Rolles
K
K
Hamilton
G
G
D000241:Adenosine; D000493:Allopurinol; D000818:Animals; D001013:Aorta, Thoracic; D015925:Cryopreservation; D011464:Epoprostenol / Q000096:biosynthesis*; D005978:Glutathione; D007328:Insulin; D008297:Male; D009926:Organ Preservation; D019188:Organ Preservation Solutions; D011887:Raffinose; D051381:Rats; D011919:Rats, Inbred Strains; D011942:Receptors, Adrenergic, alpha / Q000502:physiology*; D012996:Solutions; D013997:Time Factors
D007328:Insulin; D019188:Organ Preservation Solutions; D011942:Receptors, Adrenergic, alpha; D012996:Solutions; C055206:University of Wisconsin-lactobionate solution; D000493:Allopurinol; D011464:Epoprostenol; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose