title: Effect of the radioprotector WR2721 on irradiation-induced injury to ciliated cells of eustachian tube.
abstract: Our previous studies revealed that injury to the ciliated cells of the eustachian tube may be the primary cause of irradiation-induced serous otitis media. The purpose of this study was to investigate the effects of the radioprotector WR2721 on irradiation-induced injury to ciliated cells of the eustachian tube (ET) in chinchillas. Twelve chinchillas were divided into two groups: the control group and the experimental group, which was pretreated with a single intraperitoneal dose of the radioprotector S-2-[3-aminopropylamino]ethylphosphorothioic acid (WR2721) 400 mg/kg. The two groups were exposed to 30 Gy of 13-MeV electrons in a single fraction to the area of the bullae and nasopharynx. Ciliary dysfunction was tested and ciliated cells of the ET were examined by scanning and transmission electron microscopy. Pretreatment with WR2721 was found to protect ciliated cells of the ET from irradiation injury.
authors.affiliation: Department of Surgery, Loma Linda University School of Medicine, California.
authors.forename: D
authors.initials: D
authors.lastname: Panossian
authors.forename: T T
authors.initials: TT
authors.lastname: Jung
authors.forename: D
authors.initials: D
authors.lastname: Weeks
authors.forename: J
authors.initials: J
authors.lastname: Sandefur
authors.forename: Y M
authors.initials: YM
authors.lastname: Park
authors.forename: S K
authors.initials: SK
authors.lastname: Miller
authors.forename: K A
authors.initials: KA
authors.lastname: Krohn
mesh_terms: D004999:Amifostine / Q000494:pharmacology*; D000818:Animals; D002682:Chinchilla; D002923:Cilia / Q000528:radiation effects / Q000648:ultrastructure; D005064:Eustachian Tube / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D009079:Mucociliary Clearance / Q000528:radiation effects; D010034:Otitis Media with Effusion / Q000473:pathology / Q000517:prevention & control*; D011292:Premedication; D011833:Radiation Injuries, Experimental / Q000473:pathology / Q000517:prevention & control*
chemical_list: D004999:Amifostine