title: The HIV-1 surface protein gp120 has no effect on transmembrane signal transduction in T cells.
abstract: The ability of HIV-1 envelope glycoprotein gp120 to induce transmembrane signaling processes in human T cells and tumor T-cell lines was investigated. Differently glycosylated gp120 preparations were characterized with respect to their purity, the fraction of native gp120, and the affinity of the gp120-CD4 interaction. These data were used to establish experimental conditions that allow a substantial fraction of the CD4 receptor to be complexed with gp120 in the course of the experiments. The results are in contrast to several previous studies since no effect of gp120 on the intracellular Ca2+ concentration, the metabolism of inositol phosphates and arachidonic acid, protein kinase C translocation, and tyrosine phosphorylation was found. Cross-linking of the gp120:CD4 complex by anti-gp120 antibodies did not elicit additional effects.
authors.affiliation: Institute of Pharmacology and Toxicology, Humboldt University, Berlin, Germany.
authors.forename: R
authors.initials: R
authors.lastname: Kaufmann
authors.forename: D
authors.initials: D
authors.lastname: Laroche
authors.forename: K
authors.initials: K
authors.lastname: Buchner
authors.forename: F
authors.initials: F
authors.lastname: Hucho
authors.forename: C
authors.initials: C
authors.lastname: Rudd
authors.forename: C
authors.initials: C
authors.lastname: Lindschau
authors.forename: P
authors.initials: P
authors.lastname: Ludwig
authors.forename: A
authors.initials: A
authors.lastname: Höer
authors.forename: E
authors.initials: E
authors.lastname: Oberdisse
authors.forename: J
authors.initials: J
authors.lastname: Kopp
mesh_terms: D001095:Arachidonic Acids / Q000378:metabolism; D015704:CD4 Antigens / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D000242:Cyclic AMP / Q000096:biosynthesis; D015699:HIV Envelope Protein gp120 / Q000502:physiology*; D015497:HIV-1 / Q000502:physiology*; D006801:Humans; D007295:Inositol Phosphates / Q000378:metabolism; D007700:Kinetics; D010766:Phosphorylation; D011493:Protein Kinase C / Q000378:metabolism; D015398:Signal Transduction; D013601:T-Lymphocytes / Q000378:metabolism / Q000382:microbiology / Q000502:physiology*; D014443:Tyrosine / Q000378:metabolism
chemical_list: D001095:Arachidonic Acids; D015704:CD4 Antigens; D015699:HIV Envelope Protein gp120; D007295:Inositol Phosphates; D014443:Tyrosine; D000242:Cyclic AMP; D011493:Protein Kinase C; D002118:Calcium