Detection of p53 gene mutations in human ovarian and endometrial cancers by polymerase chain reaction-single strand conformation polymorphism analysis.
The presence of mutations in the p53 gene was examined in ovarian cancers by a polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) analysis. The primers were designed to amplify exons 5 through 9 that contain phylogenetically conserved domains of the p53 gene. Mutations were detected in 5 out of 10 cases, one of which contained a deletion in the second allele. A single base substitution was detected in 4 cases at codons 162, 175, 205 and 273 and a single base insertion in one case within codon 315. A high frequency of p53 mutations in ovarian cancers and lack of mutation in 6 benign ovarian tumors and 2 normal ovaries suggested that the mutation of the p53 gene was associated with the genesis and/or progression of ovarian cancer. In 1 of 7 endometrial cancers, two mutations at codons 239 and 254 were detected.
Naito
M
M
Department of Gynecology and Obstetrics, School of Medicine, Kyoto University.
Satake
M
M
Sakai
E
E
Hirano
Y
Y
Tsuchida
N
N
Kanzaki
H
H
Ito
Y
Y
Mori
T
T
D000483:Alleles; D001483:Base Sequence; D002872:Chromosome Deletion; D004247:DNA / Q000032:analysis / Q000235:genetics; D004273:DNA, Neoplasm / Q000032:analysis* / Q000235:genetics; D004277:DNA, Single-Stranded / Q000032:analysis* / Q000235:genetics; D004279:DNA, Viral / Q000032:analysis; D016889:Endometrial Neoplasms / Q000235:genetics* / Q000382:microbiology / Q000556:secondary; D005091:Exons / Q000235:genetics; D005260:Female; D016158:Genes, p53 / Q000235:genetics*; D016679:Genome, Viral; D006801:Humans; D008969:Molecular Sequence Data; D009154:Mutation; D009690:Nucleic Acid Conformation; D010051:Ovarian Neoplasms / Q000235:genetics* / Q000382:microbiology / Q000556:secondary; D027383:Papillomaviridae / Q000235:genetics; D010920:Placenta / Q000737:chemistry / Q000502:physiology; D016133:Polymerase Chain Reaction / Q000379:methods*; D011110:Polymorphism, Genetic / Q000235:genetics*; D011247:Pregnancy
D004273:DNA, Neoplasm; D004277:DNA, Single-Stranded; D004279:DNA, Viral; D004247:DNA