Bovine virus diarrhoea virus induces in vitro a proliferative response of peripheral blood mononuclear cells from cattle immunized by infection.
The ability of bovine peripheral blood mononuclear cells (PBMC) to mount a proliferative response to bovine virus diarrhoea virus (BVDV) in vitro was examined. After culturing PBMC in the presence of a non-cytopathic strain of BVDV for 6 days, the magnitude of PBMC proliferation was measured as incorporation of radiolabelled thymidine, present during the last 18 h. Live, but not heat-inactivated, BVDV evoked a proliferative response of PBMC obtained from cattle seropositive to the virus. However, PBMC from seronegative or persistently BVDV-infected animals were not stimulated by BVDV. The presence of live BVDV did not alter the proliferative response of PBMC to stimulation with concanavalin A.
Larsson
B
B
Department of Cattle and Sheep Diseases, Swedish University of Agricultural Sciences, Uppsala.
Fossum
C
C
D000818:Animals; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000276:immunology*; D002417:Cattle; D002478:Cells, Cultured; D001908:Diarrhea Viruses, Bovine Viral / Q000276:immunology*; D004306:Dose-Response Relationship, Immunologic; D006358:Hot Temperature; D007111:Immunity, Cellular; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000382:microbiology*; D008213:Lymphocyte Activation; D014461:Ultracentrifugation