title: Receptors for prostaglandins F2 alpha and E2 in ovine corpora lutea during maternal recognition of pregnancy.
abstract: Plasma membrane receptors for prostaglandins (PG) F2 alpha and E2 were quantified in ovine corpora lutea obtained from nonpregnant and pregnant ewes on Days 10, 13, and 15 post-estrus, and from additional ewes on Days 25 and 40 of pregnancy. Regardless of reproductive status or day post-estrus, concentrations of luteal receptors for PGF2 alpha were 7- to 10-fold greater than those for PGE2. In pregnant ewes the concentration of receptors for PGF2 alpha was highest on Day 10 (35.4 +/- 2.8 fmol/mg) and lowest on Day 25 (22.3 +/- 2.5 fmol/mg). A difference in the concentration of luteal receptors for PGF2 alpha between pregnant and nonpregnant ewes was apparent only on Day 15 post-estrus, at which time the concentration of receptors for PGF2 alpha was higher in pregnant ewes than in nonpregnant ewes (27.1 +/- 2.7 vs. 17.7 +/- 2.7 fmol/mg). Concentrations of receptors for PGE2 in pregnant ewes were similar (p > 0.05; 2.8 +/- 0.3 to 3.7 +/- 0.2 fmol/mg) between Days 13 and 40 but were higher (p < 0.05) than in corpora lutea obtained from nonpregnant ewes on Days 10 (5.0 +/- 0.4 vs. 4.1 +/- 0.2 fmol/mg) and 15 (3.7 +/- 0.2 vs. 2.0 +/- 0.4 fmol/mg) post-estrus. Although concentrations of receptors for both PGF2 alpha and PGE2 were lowest in corpora lutea obtained from nonpregnant ewes on Day 15, this was not due to luteal regression since the weights and concentrations of progesterone in corpora lutea on Day 15 were not lower than those for corpora lutea obtained on Days 10 and 13.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Physiology, Colorado State University, Fort Collins 80523.
authors.forename: G J
authors.initials: GJ
authors.lastname: Wiepz
authors.forename: M C
authors.initials: MC
authors.lastname: Wiltbank
authors.forename: T M
authors.initials: TM
authors.lastname: Nett
authors.forename: G D
authors.initials: GD
authors.lastname: Niswender
authors.forename: H R
authors.initials: HR
authors.lastname: Sawyer
mesh_terms: D000818:Animals; D003080:Cold Temperature; D003338:Corpus Luteum / Q000033:anatomy & histology / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004971:Estrus / Q000378:metabolism; D005260:Female; D006150:Guanine Nucleotides / Q000494:pharmacology; D009929:Organ Size; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000378:metabolism*; D011453:Prostaglandins / Q000378:metabolism; D011982:Receptors, Prostaglandin / Q000096:biosynthesis*; D018078:Receptors, Prostaglandin E; D012756:Sheep; D013997:Time Factors
chemical_list: D006150:Guanine Nucleotides; D011453:Prostaglandins; D011982:Receptors, Prostaglandin; D018078:Receptors, Prostaglandin E; C034671:prostaglandin F2alpha receptor