The EGF-inducible protein EIP-1 of migrating normal and malignant rat liver epithelial cells is identical to plasminogen activator inhibitor 1 and is a component of the ECM migration tracks.
Induction of rat liver epithelial cell migration by epidermal growth factor (EGF) changes the expression pattern of secreted proteins. The expression of the early induced glycoprotein EGF-inducible protein No. 1 (EIP-1) correlates with the migratory behavior of both normal and Ha-ras-transformed, tumorigenic cells and is deposited into the ECM migration tracks. The sequence of two clones from a cDNA library of EGF-induced cells and the amino terminal sequence of the purified protein revealed that EIP-1 is identical to rat plasminogen activator inhibitor 1 (PAI-1). Based on the migration-linked expression pattern of EIP-1/PAI-1 it is proposed that the inhibitor is required for the migration of these cells, but not sufficient to stimulate it.
Seebacher
T
T
Arbeitsgruppe Zellbiologie, Fakultät für Biologie, Universität Konstanz, Federal Republic of Germany.
Manske
M
M
Zoller
J
J
Crabb
J
J
Bade
E G
EG
D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D002461:Cell Line, Transformed; D002465:Cell Movement; D002478:Cells, Cultured; D003907:Dexamethasone / Q000494:pharmacology; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D005109:Extracellular Matrix / Q000737:chemistry*; D008099:Liver / Q000166:cytology* / Q000378:metabolism; D008114:Liver Neoplasms, Experimental / Q000378:metabolism / Q000473:pathology*; D017395:Plasminogen Activator Inhibitor 1 / Q000032:analysis / Q000096:biosynthesis / Q000737:chemistry*; D014176:Protein Biosynthesis; D011506:Proteins / Q000032:analysis / Q000737:chemistry*; D051381:Rats
D017395:Plasminogen Activator Inhibitor 1; D011506:Proteins; D004815:Epidermal Growth Factor; D003994:Bucladesine; D003907:Dexamethasone