title: Rapid detection of respiratory viruses using mixtures of monoclonal antibodies on shell vial cultures.
abstract: Eleven hundred and thirty-three clinical specimens submitted to the laboratory for diagnosis of respiratory virus infections were tested by direct immunofluorescence (DIF) for respiratory syncytial virus (RSV), by shell vial culture, and by conventional cell culture. The shell vial cultures were stained with 8 different monoclonal antibodies both 1 day and 3-7 days after inoculation. In order to limit the cost and the workload, mixtures of monoclonal antibodies were used. Coverslips with HEp-2 cells were incubated with a mixture of FITC-labeled monoclonal antibody to RSV and nonlabeled monoclonal antibody to adenovirus. When no RSV positive IF staining was observed after the first incubation step, the same coverslip was incubated once more with FITC-labeled anti-mouse antibody. A positive reaction at this stage indicated the presence of adenovirus. Similarly, cultures of tertiary monkey kidney cells were investigated with a mixture of two FITC-labeled monoclonals to the influenza viruses A and B and three nonlabeled monoclonals to the parainfluenza viruses 1, 2 and 3. If influenza virus or parainfluenza virus was detected, the exact type was determined by staining different parts of a duplicate coverslip. Shell vial cultures for cytomegalovirus (CMV) were always performed separately on human embryonic lung fibroblasts. Using this approach, we detected RSV (n = 248), CMV (n = 42), parainfluenza virus (n = 31), influenza virus (n = 28), and adenovirus (n = 6), in most cases after only one day of culture. For RSV, the sensitivity of the shell vial method was too low (74%) to allow omission of DIF (sensitivity 95%).(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Regional Public Health Laboratory, University Hospital, Groningen, The Netherlands.
authors.forename: J
authors.initials: J
authors.lastname: Schirm
authors.forename: D S
authors.initials: DS
authors.lastname: Luijt
authors.forename: G W
authors.initials: GW
authors.lastname: Pastoor
authors.forename: J M
authors.initials: JM
authors.lastname: Mandema
authors.forename: F P
authors.initials: FP
authors.lastname: Schröder
mesh_terms: D000258:Adenovirus Infections, Human / Q000175:diagnosis; D000911:Antibodies, Monoclonal; D003586:Cytomegalovirus Infections / Q000175:diagnosis; D005069:Evaluation Studies as Topic; D005455:Fluorescent Antibody Technique; D006801:Humans; D007251:Influenza, Human / Q000175:diagnosis; D018184:Paramyxoviridae Infections / Q000175:diagnosis; D012136:Respiratory Syncytial Viruses; D012141:Respiratory Tract Infections / Q000175:diagnosis* / Q000382:microbiology; D010253:Respirovirus Infections / Q000175:diagnosis; D012680:Sensitivity and Specificity; D014773:Virology / Q000379:methods* / Q000706:statistics & numerical data; D014777:Virus Diseases / Q000175:diagnosis* / Q000382:microbiology
chemical_list: D000911:Antibodies, Monoclonal