Adoptive transfer of V beta 2-deleting activity with host cells from mice implanted with C4 preneoplastic hyperplastic alveolar nodules.
Selective deletion of mature peripheral V beta 2+ T-cells was observed in BALB/c mice implanted with the syngeneic C4 preneoplastic hyperplastic alveolar nodule (HAN) but not in mice with sham surgery (W. Z. Wei, R. Ficsor-Jacobs, S. J. Tsai, and R. Pauley, Cancer Res., 51:3331-3333, 1991). We now report the participation of host cells in that process. Peripheral V beta 2+ T-cells were reduced by 50% or more 4 weeks after an i.v. injection of 10 x 10(6) spleen cells from C4 HAN-bearing mice. Both T- and B-cell-enriched splenocytes mediated V beta 2 deletion. Secondary adoptive transfer of splenocytes from the recipients of the primary adoptive transfer also resulted in V beta 2+ T-cell deletion. The splenocytes lose V beta 2-deleting activity after 500 rad irradiation. V beta 2 deletion induced by either C4 HAN or splenocytes was more profound in CD4+ than in CD8+ T-cells. Loss of V beta 2+CD4+ T-cells was observed 5 days after the adoptive transfer of splenocytes, whereas V beta 2+ CD8+ cells were not reduced until day 9 or later. The differential rate of V beta 2+ CD4+ and CD8+ T-cell reduction continued for at least 7 weeks after the adoptive transfer. The pattern of V beta 2 deletion and the sequence of T-cell loss is similar in the recipients of C4 HAN or of adoptively transferred splenocytes. Southern blot analysis demonstrates non-germ line Mtv or MMTV proviral DNA in C4 HAN. Splenocytes of C4 HAN-bearing mice express a higher level of 1.7-kilobase long terminal repeat transcript than normal BALB/c splenocytes, suggesting a role for a unique Mtv/MMTV provirus in V beta 2 deletion.
Wei
W Z
WZ
E. Walter Albachten Department of Immunology, Michigan Cancer Foundation, Detroit 48201.
Wang
H
H
Ficsor-Jacobs
R
R
Pauley
R
R
D000818:Animals; D001402:B-Lymphocytes / Q000378:metabolism / Q000502:physiology; D004279:DNA, Viral / Q000235:genetics; D005260:Female; D015870:Gene Expression / Q000235:genetics; D006965:Hyperplasia; D016219:Immunotherapy, Adoptive; D008175:Lung Neoplasms / Q000276:immunology* / Q000473:pathology; D008212:Lymphocyte Depletion; D008324:Mammary Tumor Virus, Mouse / Q000235:genetics / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C; D009368:Neoplasm Transplantation; D011230:Precancerous Conditions / Q000276:immunology* / Q000382:microbiology / Q000473:pathology; D011533:Proviruses / Q000235:genetics / Q000502:physiology; D011650:Pulmonary Alveoli / Q000276:immunology / Q000473:pathology*; D011836:Radiation Tolerance; D013154:Spleen / Q000166:cytology / Q000276:immunology / Q000528:radiation effects; D016176:T-Lymphocyte Subsets / Q000276:immunology; D013601:T-Lymphocytes / Q000378:metabolism / Q000502:physiology* / Q000528:radiation effects
D004279:DNA, Viral