title: The m gamma delta-1 element, a small gamma delta (Tn1000) derivative useful for plasmid mutagenesis, allele replacement and DNA sequencing.
abstract: Transposon gamma delta (Tn1000), a 6-kb member of the Tn3 family, is widely used for plasmid mutagenesis. A 1.8-kb derivative of gamma delta was constructed that contains the kan gene from Tn5 and the resolution (res) site from gamma delta cloned between 40-bp inverted repeats of gamma delta's delta (delta) end. This element, named m gamma delta-1, lacks the genes encoding transposase and resolvase, and therefore depends on its host to supply transposition and resolution functions. Thus, in strains lacking gamma delta, m gamma delta-1 will not transpose. The m gamma delta-1 element is shown to be useful for mutagenesis of plasmids, DNA sequencing, and allele replacement (in Streptomyces avermitilis).
authors.affiliation: Department of Molecular and Cell Biology, University of Connecticut, Storrs 06269-2131.
authors.forename: C M
authors.initials: CM
authors.lastname: Berg
authors.forename: N B
authors.initials: NB
authors.lastname: Vartak
authors.forename: G
authors.initials: G
authors.lastname: Wang
authors.forename: X
authors.initials: X
authors.lastname: Xu
authors.forename: L
authors.initials: L
authors.lastname: Liu
authors.forename: D J
authors.initials: DJ
authors.lastname: MacNeil
authors.forename: K M
authors.initials: KM
authors.lastname: Gewain
authors.forename: L A
authors.initials: LA
authors.lastname: Wiater
authors.forename: D E
authors.initials: DE
authors.lastname: Berg
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000235:genetics*; D005838:Genotype; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D009713:Nucleotidyltransferases / Q000235:genetics*; D009838:Oligodeoxyribonucleotides; D010957:Plasmids; D015183:Restriction Mapping; D013302:Streptomyces / Q000235:genetics*; D019895:Transposases
chemical_list: D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D009838:Oligodeoxyribonucleotides; D009713:Nucleotidyltransferases; D019895:Transposases