[Clinical significance of oncogene product expression in human lung cancer].
The clinical importance of ras oncogene product p21 was evaluated in surgically treated non-small cell lung cancer patients. Paraffin sections of tumors were analysed immunohistochemically using anti-ras p21 monoclonal antibody rp35. The ras p21 expression was correlated with clinicopathological parameters and survival. Survival analysis demonstrated significantly longer survival times in patients with p21-negative tumors than those with p21-positive tumors. In Cox's multivariate analysis, ras p21 expression was a major and independent prognostic determinant of survival. On the other hand, in small cell lung cancer, L-myc gene is known to be frequently amplified and overexpressed. Immunoprecipitation analysis of two small cell lung cancer cell lines (classic type) revealed three major L-myc proteins (p60, p66 and p68), all of which were derived from extensive phosphorylation of a p59 protein. Expression and phosphorylation of L-myc protein, as well as the autocrine growth mechanism of gastrin-releasing peptide (GRP), is thought to be involved in the malignant behavior of small cell lung cancer.
Dosaka-Akita
H
H
First Department of Medicine, Hokkaido University School of Medicine, Sapporo, Japan.
Harada
M
M
Miyamoto
H
H
Kawakami
Y
Y
D000230:Adenocarcinoma / Q000235:genetics / Q000401:mortality; D018288:Carcinoma, Small Cell / Q000235:genetics / Q000401:mortality; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000401:mortality; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000401:mortality*; D009367:Neoplasm Staging; D015689:Oncogene Protein p21(ras) / Q000032:analysis*; D011379:Prognosis; D016271:Proto-Oncogene Proteins c-myc / Q000032:analysis*; D015996:Survival Rate
D016271:Proto-Oncogene Proteins c-myc; D015689:Oncogene Protein p21(ras)