Activation of guinea pig eosinophil respiratory burst by leukotriene B4: role of protein kinase C.
Leukotriene B4 (LTB4) and the protein kinase C activator, 4-beta-phorbol dibutyrate (PDBu), both induced a pronounced and concentration-dependent stimulation of hydrogen peroxide (H2O2) generation by purified guinea pig peritoneal eosinophils in the concentration range 1 nM-1 microM. The LTB4 response was inhibited competitively by the specific LTB4 receptor antagonist, U-75302, with a KB of 25 nM, while the concentration-response curves for both stimuli were shifted rightwards (3.8-fold and 2.8-fold for LTB4 and PDBu, respectively) by the competitive protein kinase C inhibitor, 1-O-hexadecyl-2-O-methylglycerol at a concentration of 300 microM. LTB4 appears, therefore, to induce respiratory burst in eosinophils via a receptor-mediated mechanism involving protein kinase C.
Rabe
K F
KF
Department of Thoracic Medicine, National Heart and Lung Institute, London, UK.
Giembycz
M A
MA
Dent
G
G
Barnes
P J
PJ
D000818:Animals; D004804:Eosinophils / Q000187:drug effects* / Q000378:metabolism; D005233:Fatty Alcohols / Q000494:pharmacology; D005995:Glyceryl Ethers / Q000494:pharmacology; D006018:Glycols / Q000494:pharmacology; D006168:Guinea Pigs; D007975:Leukotriene B4 / Q000378:metabolism / Q000494:pharmacology*; D008297:Male; D015240:Phorbol 12,13-Dibutyrate / Q000494:pharmacology; D011493:Protein Kinase C / Q000502:physiology*; D016897:Respiratory Burst / Q000187:drug effects*
D005233:Fatty Alcohols; D005995:Glyceryl Ethers; D006018:Glycols; C059039:1-O-hexadecyl-2-O-methylglycerol; C062137:U 75302; D007975:Leukotriene B4; D015240:Phorbol 12,13-Dibutyrate; D011493:Protein Kinase C