[Interaction of (3H)naloxone with immunocompetent murine cells: the effect of mitogenic and antigenic activation].
The binding of the opioid antagonist [3H]-naloxone to immunocompetent cells of the mouse, F1(CBA x C57B1/6), in medium 199 has been studied. The binding was reversible and reached a maximum during 15-20 min at 37 degrees C. The stereospecificity profile was proven to correspond to mu-type receptors. The binding curve was characterized by high positive cooperativity (nH = 2.3, IC50 = 5 nM). Mitogenic stimulation by Con A, SEA, and ML caused an increase in the number of receptors. Besides, stimulation by an antigen (ovalbumin) changed the binding parameters. The distribution of binding sites for naloxone on various immunocompetent cells was investigated. The maximal number of sites was found on lymphocytes of lymph nodes and bone marrow cells. A conclusion is drawn that both T- and non-T-cells play a role in naloxone binding.
Khegaĭ
L A
LA
Kim
B B
BB
Gavrilova
E M
EM
Zakharova
L A
LA
Zaĭtsev
S V
SV
D000818:Animals; D000941:Antigens / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology / Q000378:metabolism*; D005260:Female; D008221:Lymphoid Tissue / Q000166:cytology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008934:Mitogens; D009270:Naloxone / Q000378:metabolism*; D011957:Receptors, Opioid / Q000378:metabolism; D013379:Substrate Specificity; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism*; D014316:Tritium
D000941:Antigens; D008934:Mitogens; D011957:Receptors, Opioid; D014316:Tritium; D009270:Naloxone
Vzaimodeĭstvie (3H)naloksona s immunokompetentnymi kletkami myshi: vliianie mitogennoĭ i antigennoĭ aktivatsii.