The regulatory subunit of the type II cAMP-dependent protein kinase in rabbit ovaries is the RII beta isoform.
Based on RII autophosphorylation, photoaffinity labeling with 8-N3[32P]cAMP, and Western blot analysis we have identified the RII isoform found in rabbit corpora lutea as RII beta. The RII beta subunit found in rabbit corpora lutea differs from the RII beta found in rat follicles and corpora lutea in that it migrates at Mr 52,500 on SDS-PAGE and shifts to Mr 53,000 when phosphorylated.
Jackiw
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V
Department of Cell, Molecular, and Structural Biology, Northwestern University Medical School, Chicago, IL 60611.
Hunzicker-Dunn
M
M
D000345:Affinity Labels / Q000378:metabolism; D000818:Animals; D000936:Antigen-Antibody Complex; D001386:Azides / Q000378:metabolism; D015153:Blotting, Western; D002848:Chromatography, DEAE-Cellulose; D003338:Corpus Luteum / Q000201:enzymology*; D000242:Cyclic AMP / Q000031:analogs & derivatives / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D007527:Isoenzymes / Q000302:isolation & purification* / Q000378:metabolism; D046911:Macromolecular Substances; D008970:Molecular Weight; D010053:Ovary / Q000201:enzymology*; D010766:Phosphorylation; D011494:Protein Kinases / Q000302:isolation & purification* / Q000378:metabolism; D011817:Rabbits
D000345:Affinity Labels; D000936:Antigen-Antibody Complex; D001386:Azides; D007527:Isoenzymes; D046911:Macromolecular Substances; C017042:8-azidoadenosine-3',5'-monophosphate; D000242:Cyclic AMP; D011494:Protein Kinases