title: Insulin and isoproterenol induce phosphorylation of the particulate cyclic GMP-inhibited, low Km cyclic AMP phosphodiesterase (cGI PDE) in 3T3-L1 adipocytes.
abstract: The cGI PDE in particulate fractions of differentiated adipocytes (but not control 3T3-L1 fibroblasts) was cross-reactive with a polyclonal antibody raised against the bovine adipose cGI PDE. The 3T3-L1 adipocyte cGI PDE is a 135 kDa protein which is phosphorylated in 32P-labeled cells in response to beta-agonist or insulin. These results indicate that the 3T3-L1 cGI PDE is similar in structure and hormonal regulation to the analogous enzyme in the rat adipocyte.
authors.affiliation: Laboratory of Cellular Metabolism, National Heart, Lung and Blood Institute, NIH, Bethesda, MD 20892.
authors.forename: V
authors.initials: V
authors.lastname: Vasta
authors.forename: C J
authors.initials: CJ
authors.lastname: Smith
authors.forename: J
authors.initials: J
authors.lastname: Calvo
authors.forename: P
authors.initials: P
authors.lastname: Belfrage
authors.forename: V C
authors.initials: VC
authors.lastname: Manganiello
mesh_terms: D016475:3T3 Cells / Q000187:drug effects / Q000201:enzymology; D000273:Adipose Tissue / Q000187:drug effects / Q000196:embryology* / Q000378:metabolism; D000818:Animals; D015153:Blotting, Western; D002454:Cell Differentiation / Q000187:drug effects; D003429:Cross Reactions; D007328:Insulin / Q000494:pharmacology*; D007545:Isoproterenol / Q000494:pharmacology*; D051379:Mice; D010766:Phosphorylation; D011494:Protein Kinases / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*; D015363:Quinolones / Q000494:pharmacology
chemical_list: D007328:Insulin; D015363:Quinolones; C021294:cilostamide; D011494:Protein Kinases; D007545:Isoproterenol