title: DNA supercoiling facilitates the assembly of transcriptionally active chromatin on the adenovirus major late promoter.
abstract: Assembly of nucleosomes on the adenovirus major late promoter blocked initiation of transcription by RNA polymerase II. However, the formation of transcription preinitiation complexes prevented subsequent assembly of promoter sequences into nucleosomes and allowed transcription on the chromatin templates. When the formation of preinitiation complexes was in competition with nucleosome assembly, transcription on linear or relaxed closed circular DNA was inactivated by nucleosome assembly over the promoter region. However, transcription on partially supercoiled DNA (mean superhelical density of -0.036) remained active because the rapid formation of preinitiation complexes prevented subsequent assembly of promoter sequences into nucleosomes.
authors.affiliation: Department of Genetics, Graduate University for Advanced Studies, Mishima, Japan.
authors.forename: R
authors.initials: R
authors.lastname: Ohba
authors.forename: H
authors.initials: H
authors.lastname: Tabuchi
authors.forename: S
authors.initials: S
authors.lastname: Hirose
mesh_terms: D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D002417:Cattle; D002843:Chromatin / Q000378:metabolism*; D004264:DNA Topoisomerases, Type I / Q000378:metabolism; D004270:DNA, Circular / Q000235:genetics / Q000378:metabolism*; D004278:DNA, Superhelical / Q000378:metabolism*; D004279:DNA, Viral / Q000235:genetics / Q000378:metabolism*; D006367:HeLa Cells; D006657:Histones / Q000378:metabolism; D006801:Humans; D009707:Nucleosomes / Q000378:metabolism; D011401:Promoter Regions, Genetic; D012319:RNA Polymerase II / Q000302:isolation & purification / Q000378:metabolism; D013950:Thymus Gland / Q000201:enzymology; D035581:Transcription Factor TFIIB; D014157:Transcription Factors / Q000302:isolation & purification / Q000378:metabolism; D019473:Transcription Factors, TFII; D014158:Transcription, Genetic
chemical_list: D002843:Chromatin; D004270:DNA, Circular; D004278:DNA, Superhelical; D004279:DNA, Viral; D006657:Histones; D009707:Nucleosomes; D035581:Transcription Factor TFIIB; D014157:Transcription Factors; D019473:Transcription Factors, TFII; C066644:transcription factor TFIIE; D012319:RNA Polymerase II; D004264:DNA Topoisomerases, Type I