Interferon-gamma-like immunoreactivity in sensory neurons may influence the replication of Sendai and mumps viruses.
Rat dorsal root ganglia in tissue culture, which contain an interferon-gamma (IFN-gamma)-like immunoreactive subpopulation of neurons, were infected with paramyxoviruses. Sendai virus caused a substantial neuronal lysis, while the RW strain of mumps virus caused a much less pronounced nerve cell loss. Early during infection, the subpopulation of IFN-gamma-like immunoreactive neurons was less susceptible to mumps virus. Virus antigen was rapidly lost from surviving IFN-gamma-like positive neurons infected with Sendai virus, while this remarkable self-curing effect occurred in both nerve cell populations at later time points after mumps virus infection. By quantitative enzyme-linked immunosorbent assay (ELISA) technique, increased levels of "neuronal IFN-gamma" were recorded at 10 hr and 30 hr after infection with Sendai and mumps virus, respectively. This study indicates a role for the neuronal IFN-gamma-like molecule in determining the outcome of a viral infection in sensory ganglia.
Eneroth
A
A
Division of Basic Research in Dementia, Karolinska Institutet, Huddinge Hospital, Sweden.
Andersson
T
T
Olsson
T
T
Orvell
C
C
Norrby
E
E
Kristensson
K
K
D000818:Animals; D000956:Antigens, Viral / Q000276:immunology; D046508:Culture Techniques; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005727:Ganglia, Spinal / Q000166:cytology; D007150:Immunohistochemistry; D007371:Interferon-gamma / Q000276:immunology / Q000378:metabolism* / Q000502:physiology; D009109:Mumps virus / Q000502:physiology*; D009410:Nerve Degeneration; D009475:Neurons, Afferent / Q000276:immunology / Q000378:metabolism* / Q000502:physiology; D010222:Parainfluenza Virus 1, Human / Q000502:physiology*; D011247:Pregnancy; D051381:Rats; D011919:Rats, Inbred Strains; D014779:Virus Replication / Q000502:physiology*
D000956:Antigens, Viral; D007371:Interferon-gamma