[Dynamic changes of pulmonary glucocorticoid receptor in endotoxin-induced lung injury].
The changes of maximal binding capacity (Bmax) of glucocorticoid receptor (GCR) in the endotoxin-induced lung injury were observed by using radioligand binding assay. The content of glucocorticoid (GC) and the activity of phospholipase A2 (PLA2) were also measured. The results showed that the level of GCR in lung cytoplasma decreased continuously after endotoxin infusion, and its affinity decreased in the late phase while PLA2 activity markedly increased. There was a negative correlation between the Bmax of GCR and PLA2 activity (r = -0.87, P < 0.01). These findings indicated that there is a secondary alteration in GCR during endo toxin-induced lung injury, and that the GC hypofunction caused by reduced GCR binding capacity may be of importance in the development of ARDS.
Liu
L
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Department of Respiration, Xijing Hospital, Fourth Military Medical University, Xian.
D000818:Animals; D004731:Endotoxins; D004926:Escherichia coli; D005260:Female; D005938:Glucocorticoids / Q000097:blood; D008168:Lung / Q000378:metabolism* / Q000473:pathology; D008171:Lung Diseases / Q000209:etiology / Q000378:metabolism*; D010741:Phospholipases A / Q000378:metabolism; D054467:Phospholipases A2; D011869:Radioligand Assay; D011965:Receptors, Glucocorticoid / Q000378:metabolism*; D012756:Sheep
D004731:Endotoxins; D005938:Glucocorticoids; D011965:Receptors, Glucocorticoid; D010741:Phospholipases A; D054467:Phospholipases A2