[Apolipoprotein C III binding sites (receptor) on non-parenchymal cells from rat liver].
Binding sites of apolipoprotein (apo) C III on non-parenchymal cells (NPC) isolated from rat liver were found. Apo C III was purified from delipided human plasma very low density lipoproteins. 125I-labeled-apo C III was prepared by chloramine-T method. Non-parenchymal cells were isolated from rat liver by collagenase method. Freshly isolated rat liver NPC bound 125I-labeled apo C III in a saturable way with Kd 0.1-0.55 mumol/L and Bmax 18.1-42.0 ng/ml cell protein. There were about 1.0-4.5 x 10(5) specific binding sites of apo C III on each NPC. Unlabeled apo C III, but not apo AI and B100, could inhibit these binding activities. The results demonstrate the presence of saturable and specific apo C III receptors (binding sites) on rat liver NPC, but their nature and biologic properties remain to be investigated.
Zhang
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Liu
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D000818:Animals; D053305:Apolipoprotein C-III; D001056:Apolipoproteins C / Q000378:metabolism* / Q000502:physiology; D001665:Binding Sites; D006801:Humans; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D026503:Low Density Lipoprotein Receptor-Related Protein-1; D051381:Rats; D017207:Rats, Sprague-Dawley; D011956:Receptors, Cell Surface / Q000378:metabolism*
D053305:Apolipoprotein C-III; D001056:Apolipoproteins C; D026503:Low Density Lipoprotein Receptor-Related Protein-1; D011956:Receptors, Cell Surface