title: Partial nucleotide sequence of the enzyme pyruvate, orthophosphate dikinase of Entamoeba histolytica HM1:IMSS.
abstract: We isolated a clone from a lambda gt11 cDNA library of E. histolytica, subcloned its insert in the vector pTZ18R and sequenced it by the method of Sanger. Sequence comparison with Genbank and of the corresponding amino acid sequence with the NBRF/PIR data bank using the FastA program, showed homologies between 54.9 and 58.5% for the nucleotides and between 48.6 and 49.4% for the amino acids with pyruvate, orthophosphate dikinases from maize, Flaveria trinervia and Bacteroides symbiosus. The sequence obtained for E. histolytica represents about 20% of the complete gene or protein sequence.
authors.affiliation: Departamento de Microbiología, Instituto de Fisiología Celular, UNAM, México, DF.
authors.forename: E
authors.initials: E
authors.lastname: Saavedra Lira
authors.forename: O
authors.initials: O
authors.lastname: Robinson
authors.forename: R
authors.initials: R
authors.lastname: Pérez Montfort
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D018076:DNA, Complementary / Q000235:genetics; D016054:DNA, Protozoan / Q000235:genetics*; D004748:Entamoeba histolytica / Q000201:enzymology / Q000235:genetics*; D017125:Genes, Protozoan; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010940:Plant Proteins / Q000235:genetics; D016133:Polymerase Chain Reaction; D015800:Protozoan Proteins / Q000235:genetics*; D011772:Pyruvate, Orthophosphate Dikinase / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
chemical_list: D001426:Bacterial Proteins; D018076:DNA, Complementary; D016054:DNA, Protozoan; D010940:Plant Proteins; D015800:Protozoan Proteins; D011772:Pyruvate, Orthophosphate Dikinase