title: Bovine papillomavirus type 1-transformed primary mouse fibroblasts show no correlation between tumorigenicity and viral gene expression, but c-myc gene expression is elevated in tumorigenic cell lines.
abstract: Bovine papillomavirus type 1 (BPV-1)-transformed primary mouse fibroblasts containing episomal or integrated BPV-1 sequences were analysed for virus-specific transcripts and c-myc gene expression. Total BPV-1-specific expression was high in cell lines containing episomal BPV-1 DNA in comparison to lines containing integrated BPV-1 sequences, mainly due to higher expression of the E6/E7 sequences. No correlation was found between the viral transcription and tumorigenicity, although BPV-1 gene expression occurred in all cell lines. High levels of c-myc expression were found in all cell lines exhibiting a tumorigenic phenotype as compared to the nontumorigenic lines. These data suggest that expression of BPV-1 genes may be essential for transformation but not tumorigenicity, whereas high levels of expression of cellular oncogenes like c-myc may be associated with tumorigenicity.
authors.affiliation: Department of Clinical Microbiology, University of Kuopio, Finland.
authors.forename: R
authors.initials: R
authors.lastname: Agrawal
authors.forename: J
authors.initials: J
authors.lastname: Pelkonen
authors.forename: R A
authors.initials: RA
authors.lastname: Mäntyjärvi
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D052688:Bovine papillomavirus 1 / Q000235:genetics / Q000502:physiology; D002471:Cell Transformation, Neoplastic; D002472:Cell Transformation, Viral; D003720:Densitometry; D005347:Fibroblasts / Q000378:metabolism / Q000382:microbiology*; D015967:Gene Expression Regulation, Viral; D005814:Genes, Viral; D016259:Genes, myc; D051379:Mice; D009368:Neoplasm Transplantation; D015183:Restriction Mapping; D014407:Tumor Cells, Cultured