A high involvement of O2- possibly generated in inner membranes for iron-induced microsomal lipid peroxidation.
The lipid peroxidation of and the O2- generation by rat liver microsomes in the presence of NADPH or both NADPH and Fe3+ were determined by thiobarbituric acid-reacting substance formation and by chemiluminescence intensities with a cypridina luciferin analog, 2-methyl-6-(p-methoxyphenyl)-3, 7-dihydroimidazo[1,2-a]pyrazin-3-one(MCLA), as a chemiluminescence probe. Judging from the experiments with various inhibitors on the O2- generation and the lipid peroxidation, O2- generated, at intramembranous site, by cytochrome P-450 system is considered to be highly involved in the iron-induced lipid peroxidation.
Koga
S
S
Photon Medical Research Center, Hamamatsu University School of Medicine, Shizuoka, Japan.
Nakano
M
M
D000818:Animals; D002570:Ceruloplasmin / Q000494:pharmacology; D003300:Copper / Q000494:pharmacology; D003577:Cytochrome P-450 Enzyme System / Q000494:pharmacology; D004912:Erythrocytes / Q000201:enzymology; D016166:Free Radical Scavengers; D007093:Imidazoles / Q000494:pharmacology; D007425:Intracellular Membranes / Q000187:drug effects / Q000378:metabolism*; D007501:Iron / Q000378:metabolism* / Q000494:pharmacology; D015227:Lipid Peroxidation / Q000187:drug effects*; D008163:Luminescent Measurements; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism*; D008892:Milk / Q000201:enzymology; D008954:Models, Biological; D009942:Organometallic Compounds / Q000494:pharmacology; D011719:Pyrazines / Q000494:pharmacology; D051381:Rats; D012459:Salicylates / Q000494:pharmacology; D013482:Superoxide Dismutase / Q000494:pharmacology; D013481:Superoxides / Q000378:metabolism*; D014969:Xanthine Oxidase / Q000494:pharmacology
D016166:Free Radical Scavengers; D007093:Imidazoles; D009942:Organometallic Compounds; D011719:Pyrazines; D012459:Salicylates; C048193:2-methyl-6-phenyl-3,7-dihydroimidazo(1,2-a)pyrazin-3-one; D013481:Superoxides; C063412:2-methyl-6-(4-methoxyphenyl)-3,7-dihydroimidazo(1,2-alpha)pyrazin-3-one; C025564:copper salicylate complex; D003300:Copper; D003577:Cytochrome P-450 Enzyme System; D007501:Iron; D013482:Superoxide Dismutase; D002570:Ceruloplasmin; D014969:Xanthine Oxidase