The DNA polymerase-encoding gene of Bacillus subtilis bacteriophage SPO1.
The bacteriophage SPO1 DNA polymerase-encoding gene, which contains a self-splicing intron, has been sequenced and its amino acid (aa) sequence has been deduced. The aa sequence of SPO1 DNA polymerase shows a high degree of similarity with that of DNA polymerase I from Escherichia coli (Po1I). Alignment with the sequences of Po1I, and the phi 29 and SPO1 DNA polymerases indicate that the aa residues that have been implicated in 3'----5' exonuclease activities are conserved.
Scarlato
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International Institute of Genetics and Biophysics, Naples, Italy.
Gargano
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D000595:Amino Acid Sequence; D001412:Bacillus subtilis; D001435:Bacteriophages / Q000201:enzymology* / Q000235:genetics; D001483:Base Sequence; D004256:DNA Polymerase I / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D005092:Exonucleases / Q000378:metabolism; D007438:Introns / Q000235:genetics; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
D004256:DNA Polymerase I; D005092:Exonucleases