Human C-type natriuretic peptide. Characterization of the gene and peptide.
We isolated the human C-type natriuretic peptide gene and identified the peptide in the brain. The human C-type natriuretic peptide gene appeared to be composed of at least two exons and one intron. In the 5'-flanking region, there is an array of cis elements (an inverted CCAAT box, two GC boxes, and a cyclic AMP response element-like sequence) that is not present in upstream sequences of the atrial and brain natriuretic peptide genes. Analysis of the deduced amino acid sequence revealed that human prepro C-type natriuretic peptide comprises 126 amino acids and that the C-terminal 22-residue peptide (G-L-S-K-G-C-F-G-L-K-L-D-R-I-G-S-M-S-G-L-G-C) preceded by Lys-Lys is identical to the porcine counterpart. However, replacement of two amino acids took place in the C-terminal 53-residue sequence, corresponding to another endogenous form of the peptide. Reverse-phase high-performance liquid chromatography coupled with a radioimmunoassay for C-type natriuretic peptide demonstrated that it occurs in the human brain. C-type natriuretic peptide-like immunoreactivity was detected in discrete regions of the human brain, and its level was 10-fold higher than the atrial and brain natriuretic peptide levels, raising the possibility that C-type natriuretic peptide is the major natriuretic peptide in the human brain.
Ogawa
Y
Y
Second Division, Kyoto University School of Medicine, Japan.
Nakao
K
K
Nakagawa
O
O
Komatsu
Y
Y
Hosoda
K
K
Suga
S
S
Arai
H
H
Nagata
K
K
Yoshida
N
N
Imura
H
H
D000595:Amino Acid Sequence; D001483:Base Sequence; D001923:Brain Chemistry; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D008969:Molecular Sequence Data; D020098:Natriuretic Peptide, C-Type; D009419:Nerve Tissue Proteins / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D015345:Oligonucleotide Probes / Q000235:genetics
D009419:Nerve Tissue Proteins; D015345:Oligonucleotide Probes; D020098:Natriuretic Peptide, C-Type