Assignment of the human 2',3'-cyclic nucleotide 3'-phosphohydrolase gene to chromosome 17.
2',3'-Cyclic nucleotide 3'-phosphohydrolase (CNP) has been used as a general oligodendrocyte and Schwann cell marker enzyme within the nervous system and has been the intense target of a number of recent studies. In this report, we determined the chromosomal localization of the human CNP gene using PCR on two somatic cell DNA panels. PCR amplification, using four primer pairs across an intron, confirms that the CNP gene is localized to chromosome 17. We also present the complete intron sequence of the human gene usd to make the assignment. This intron contains a c----t polymorphism located at nucleotide 1215, which may be of use in mapping the CNPase gene more precisely within chromosome 17.
Sprinkle
T J
TJ
Department of Biochemistry, Medical College of Georgia, Augusta 30912.
Lanclos
K D
KD
Lapp
D F
DF
D063309:2',3'-Cyclic Nucleotide 3'-Phosphodiesterase; D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases / Q000235:genetics*; D001483:Base Sequence; D002874:Chromosome Mapping; D002886:Chromosomes, Human, Pair 17; D006801:Humans; D006822:Hybrid Cells; D007438:Introns; D008969:Molecular Sequence Data; D010727:Phosphoric Diester Hydrolases; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic
D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases; D010727:Phosphoric Diester Hydrolases; D063309:2',3'-Cyclic Nucleotide 3'-Phosphodiesterase; C571337:CNP protein, human