[Evaluation of the effect of the dilution of urine on the toxicity and isolation of cytomegalovirus using the rapid shell-vial centrifugation culture technique].
Working with highly passaged MRC-5 fibroblast monolayers in a shell-vial culture-centrifugation assay for rapid detection of cytomegalovirus (CMV), a direct toxic effect of the urine on the monolayer was observed in as many as 44% of cases when vials were inoculated with undiluted specimens. This toxicity was reduced to only 6% by inoculation of shell-vials with samples diluted 1:1 with viral transport medium. The recovery of CMV in shell-vials was not significantly affected by the dilution factor, since five cases detected in conventional culture in tubes and not in vials were compensated by seven cases detected in vials and not in tubes.
Pedraza
M A
MA
Unidad de Virología, Hospital 12 de Octubre, Madrid.
Otero
J R
JR
D016477:Artifacts; D002478:Cells, Cultured; D002498:Centrifugation; D003587:Cytomegalovirus / Q000302:isolation & purification*; D005347:Fibroblasts / Q000382:microbiology; D006801:Humans; D008828:Microbiological Techniques; D014556:Urine
Evalución del efecto de la dilución de la orina sobre la toxicidad y el aislamiento de citomegalovirus con la técnica rápida de cultivo-centrifugación en viales.