Effect of new-breviscapine on fibrinolysis and anticoagulation of human vascular endothelial cells.
Cultured confluent human umbilical vein endothelial cells were incubated with new-breviscapine (NB), a flavonoid consisting of 4-OH-scutellarin-7-O-glucuronide (C33H30O18) and FeCl3, MgCl2, and CaCl2, which is first extracted from Erigeron breviscapus (Vant) Hand-Mazz in China, 0, 6.25, 12.5, 25, 50, 100, and 1,000 micrograms.ml-1. The releases of tissue-type plasminogen activator (t-PA), and epoprostenol (Epo) from endothelial cells were stimulated by NB, but no significant effect of plasminogen activator inhibitor (PAI) activity was seen. NB 25-1,000 micrograms.ml-1 induced a production of thrombomodulin (TM) within the cells, an expression of TM on the surface of the cells, and a release of TM from the cells. Our data provide a new evidence that NB is a stimulant to fibrinolysis and anticoagulation of endothelial cells.
Zhou
Q S
QS
Jiangsu Institute of Hematology, Suzhou Medical College, China.
Zhao
Y M
YM
Bai
X
X
Li
P X
PX
Ruan
C G
CG
D000925:Anticoagulants / Q000494:pharmacology*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects*; D011464:Epoprostenol / Q000378:metabolism; D005342:Fibrinolysis / Q000187:drug effects*; D005419:Flavonoids; D006801:Humans; D011956:Receptors, Cell Surface / Q000378:metabolism; D018179:Receptors, Thrombin; D013917:Thrombin / Q000378:metabolism; D010959:Tissue Plasminogen Activator / Q000378:metabolism; D014471:Umbilical Veins
D000925:Anticoagulants; D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D011956:Receptors, Cell Surface; D018179:Receptors, Thrombin; C061097:breviscapine; D011464:Epoprostenol; D013917:Thrombin; D010959:Tissue Plasminogen Activator