Insertional inactivation of a gene which controls expression of vancomycin resistance on plasmid pHKK100.
Expression of inducible high level vancomycin resistance (Vmr) in enterococci appears to require other plasmid-encoded genes in addition to the previously described structural genes vanA and vanH. Tn917 mutagenesis was used to identify such a region in the Vmr plasmid pHKK100. Insertional inactivation of a 693-bp open reading frame upstream from vanH resulted in complete loss of Vmr. This putative 26,642-Da protein has been designated VanR.
Handwerger
S
S
Division of Infectious Diseases, Beth Israel Medical Center, Mount Sinai School of Medicine, New York, NY.
Discotto
L
L
Thanassi
J
J
Pucci
M J
MJ
D000595:Amino Acid Sequence; D001483:Base Sequence; D004251:DNA Transposable Elements; D004352:Drug Resistance, Microbial / Q000235:genetics*; D016983:Enterococcus / Q000187:drug effects / Q000235:genetics*; D015870:Gene Expression; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D010641:Phenotype; D011815:R Factors / Q000235:genetics*; D014640:Vancomycin / Q000494:pharmacology*
D004251:DNA Transposable Elements; D014640:Vancomycin