The product of the cellular crk gene consists primarily of SH2 and SH3 regions.
We have cloned and sequenced a complementary DNA encoding the cellular homologue of the transforming oncogene v-crk of avian sarcoma virus CT10. This complementary DNA contains an open reading frame of 915 base pairs that encodes a polypeptide of 305 amino acids. The first 205 amino acids of this c-Crk protein were identical to those of the CT10 encoded v-Crk protein, with the exception of 5 amino acids. Like v-Crk, this portion of c-Crk contained one each of the Src homology domains SH2, SH2', and SH3. The 100 carboxy-terminal amino acids of c-Crk protein, which are not coded for in the CT10 viral genome, contain another SH3 region. We found limited sequence homology between c-crk and the avian retrovirus genome, which explains recombination events in the transduction of this protooncogene. Using a polyclonal antiserum made against bacterially expressed v-crk, we identified a 35-kilodalton protein in normal chicken embryo fibroblasts and in all embryonic chicken tissues examined. This 35-kilodalton protein was indistinguishable from a polypeptide made by in vitro translation of c-crk complementary DNA.
Reichman
C T
CT
Rockefeller University, New York, New York 10021.
Mayer
B J
BJ
Keshav
S
S
Hanafusa
H
H
D000595:Amino Acid Sequence; D000818:Animals; D001358:Avian Sarcoma Viruses / Q000235:genetics; D001483:Base Sequence; D002645:Chickens / Q000235:genetics*; D004247:DNA / Q000235:genetics; D005796:Genes; D005814:Genes, Viral; D015750:Genes, gag; D008969:Molecular Sequence Data; D050736:Oncogene Protein v-crk; D016366:Open Reading Frames; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis / Q000235:genetics*; D050737:Proto-Oncogene Proteins c-crk; D015682:Retroviridae Proteins, Oncogenic / Q000235:genetics; D017385:Sequence Homology; D015678:Viral Structural Proteins / Q000235:genetics
D050736:Oncogene Protein v-crk; D011518:Proto-Oncogene Proteins; D050737:Proto-Oncogene Proteins c-crk; D015682:Retroviridae Proteins, Oncogenic; D015678:Viral Structural Proteins; D004247:DNA