Establishment of mouse oligodendrocyte/type-2 astrocyte lineage cell line by transfection with origin-defective simian virus 40 DNA.
A permanent glial cell line has been established from the neonatal mouse primary mixed glial cell cultures by transfection with replication origin-defective simian virus 40 DNA. This cell line, designated OS3, has morphological similarity to type-2 astrocyte and expresses an astrocyte-specific marker, glial fibrillary acidic protein (GFAP), when cultured in the presence of 10% calf serum (CS). OS3 cells do not express the O4 antigen, galactocerebroside (GalC) and A2B5 under this culture condition. When cultured in a medium containing 2% CS or a chemically defined medium, these cells undergo morphological transformation. Some of these cells express O4 antigen and/or GalC, and the percentage of GFAP positive cells decreases under these conditions. Thus depending on the culture conditions, the OS3 cells display either type-2 astrocyte properties or immature oligodendrocyte characteristics. Furthermore, the OS3 cells show similar responses to the various growth factors as do oligodendrocyte/type-2 astrocyte (O-2A) progenitors. Therefore, the OS3 cell line is an unique mouse bipotential permanent O-2A lineage cell line which may be useful to analyze the developmental properties of these glial cells.
Ohtani
K
K
Department of Neurology, Nagoya University School of Medicine, Japan.
Suzumura
A
A
Sawada
M
M
Marunouchi
T
T
Nakashima
I
I
Takahashi
A
A
D000818:Animals; D001253:Astrocytes / Q000502:physiology*; D001790:Blood Physiological Phenomena; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D004261:DNA Replication / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics*; D005455:Fluorescent Antibody Technique; D006133:Growth Substances / Q000494:pharmacology; D051379:Mice; D009457:Neuroglia / Q000502:physiology*; D009836:Oligodendroglia / Q000502:physiology*; D013539:Simian virus 40 / Q000235:genetics*; D013234:Stem Cells / Q000502:physiology; D014162:Transfection / Q000235:genetics*
D004279:DNA, Viral; D006133:Growth Substances