[Tetrandrine on monocyte migration induced by endothelial cell-derived chemotactic factor].
The medium conditioned by cultured human umbilical vein endothelial cells (EC-CM) was used as a source of chemotactic factor, and its chemotactic activity for monocytes was tested by using micropore filter assay in modified Boyden chamber. The effects of tetrandrine, a calcium antagonist, on monocyte migration induced by EC-CM were investigated. The results showed that cultured human umbilical vein endothelial cells secreted a chemotactic factor for monocytes, and the monocyte migration induced by this chemoattractant could be inhibited by tetrandrine at the concentration of 10(-8)-10(-5) mol/L in a dose-dependent manner. Verapamil at the concentration of x10(-8) mol/L also showed the same effect. It is suggested that tetrandrine may inhibit monocyte migration into arterial subendothelial space through antagonism to calcium and thereby suppress atherogenesis.
Zhou
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Department of Pathology, Tongji Medical University, Wuhan.
D000470:Alkaloids / Q000494:pharmacology*; D044182:Benzylisoquinolines; D002121:Calcium Channel Blockers / Q000494:pharmacology*; D002464:Cell Migration Inhibition; D002465:Cell Movement / Q000187:drug effects; D002478:Cells, Cultured; D002630:Chemotactic Factors / Q000378:metabolism / Q000494:pharmacology*; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism*; D005260:Female; D006801:Humans; D009000:Monocytes / Q000502:physiology*; D014471:Umbilical Veins
D000470:Alkaloids; D044182:Benzylisoquinolines; D002121:Calcium Channel Blockers; D002630:Chemotactic Factors; C009438:tetrandrine