Unusual structure of plasmid DNA formed in transformants of Escherichia coli.
An unusual structural component of plasmid pUC18 was observed in transformants of Escherichia coli strains. This molecule migrated faster than superhelical topoisomers in agarose gel electrophoresis and was difficult to stain with ethidium bromide. This unusual topology appeared independently of gyrase mutation, and the recA genotype may relate to accumulation of the plasmids conforming to the unusual topology.
Kato
M
M
Department of Life Sciences, College of Integrated Arts and Sciences, University of Osaka Prefecture, Sakai, Japan.
Furuno
A
A
D015139:Blotting, Southern; D004261:DNA Replication; D004250:DNA Topoisomerases, Type II / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics*; D004278:DNA, Superhelical / Q000235:genetics*; D004587:Electrophoresis, Agar Gel; D004926:Escherichia coli / Q000235:genetics* / Q000254:growth & development; D066298:In Vitro Techniques; D010957:Plasmids / Q000235:genetics*
D004269:DNA, Bacterial; D004278:DNA, Superhelical; D004250:DNA Topoisomerases, Type II