Enhanced levels of multicatalytic proteinase mRNAs in Rous sarcoma virus transformed cells.
The multicatalytic proteinase (proteasome; MCP) is a high molecular mass proteinase which is found in all eukaryotic cells. Northern blot analysis of the levels of MCP mRNAs in a Rat-1 fibroblast cell line and in cells transformed with Rous sarcoma virus showed marked increases in the transformed cells. However, the results of immunoblot analysis with anti-MCP antibodies suggested that the MCP protein content of the two cell lines was similar.
Balson
D F
DF
Department of Biochemistry, University of Leicester, U.K.
Skilton
H E
HE
Sweeney
S T
ST
Thomson
S
S
Rivett
A J
AJ
D000818:Animals; D001358:Avian Sarcoma Viruses / Q000378:metabolism*; D015152:Blotting, Northern; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D003546:Cysteine Endopeptidases / Q000378:metabolism*; D004247:DNA / Q000378:metabolism; D015342:DNA Probes; D005347:Fibroblasts / Q000378:metabolism; D009097:Multienzyme Complexes / Q000378:metabolism*; D046988:Proteasome Endopeptidase Complex; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D014158:Transcription, Genetic
D015342:DNA Probes; D009097:Multienzyme Complexes; D012333:RNA, Messenger; D004247:DNA; D003546:Cysteine Endopeptidases; D046988:Proteasome Endopeptidase Complex