title: Cooperativity in transactivation between retinoic acid receptor and TFIID requires an activity analogous to E1A.
abstract: In embryonal carcinoma (EC) cells retinoic acid (RA) strongly induces transcription from the RA receptor beta 2 (RAR beta 2) promoter through an RA response element (RARE) located in close proximity to the TATA box. Here we demonstrate that recombinant human TATA box-binding protein, hTFIID, and RAR functionally cooperate in transactivation of the RAR beta 2 promoter in EC cells in a strictly RA-dependent manner. We demonstrate that the core domain of hTFIID is sufficient to mediate RAR-dependent transcription and that Drosophila, but not yeast, TFIID can substitute for hTFIID. In COS cells ectopic expression of the E1A protein is a prerequisite for hTFIID and RAR to cooperate in transactivation. We propose a model for transcriptional regulation of the RAR beta 2 promoter in EC cells in which RAR, following activation by RA, functionally interacts with hTFIID via an E1A-like activity present in EC cells.
authors.affiliation: EMBL, Gene Expression Program, Heidelberg, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Berkenstam
authors.forename: M M
authors.initials: MM
authors.lastname: Vivanco Ruiz
authors.forename: D
authors.initials: D
authors.lastname: Barettino
authors.forename: M
authors.initials: M
authors.lastname: Horikoshi
authors.forename: H G
authors.initials: HG
authors.lastname: Stunnenberg
mesh_terms: D017456:Adenovirus Early Proteins; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002352:Carrier Proteins / Q000502:physiology*; D002460:Cell Line; D002522:Chlorocebus aethiops; D004252:DNA Mutational Analysis; D004331:Drosophila melanogaster / Q000502:physiology; D005786:Gene Expression Regulation; D006801:Humans; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D009856:Oncogene Proteins, Viral / Q000502:physiology; D011401:Promoter Regions, Genetic; D018168:Receptors, Retinoic Acid; D012045:Regulatory Sequences, Nucleic Acid; D012441:Saccharomyces cerevisiae; D013045:Species Specificity; D035362:Transcription Factor TFIID; D014157:Transcription Factors / Q000502:physiology*; D015533:Transcriptional Activation
chemical_list: D017456:Adenovirus Early Proteins; D002352:Carrier Proteins; D009856:Oncogene Proteins, Viral; D018168:Receptors, Retinoic Acid; D035362:Transcription Factor TFIID; D014157:Transcription Factors