Prevalence of transposons encoding kanamycin, ampicillin and trimethoprim resistance in isolates from urinary tract infections detected using DNA probes.
Drug resistant Gram-negative bacteria causing urinary tract infections were collected. Kanamycin, ampicillin or trimethoprim-resistant strains were analyzed separately for the presence of Tn5, Tn3, or Tn7 by colony hybridization. Of these isolates, kanamycin-resistant transposons were present in 38.2% of 60 kanamycin-resistant isolates. A 3.3 kb fragment containing SacI-BamHI transposase of Tn3 and 42.6% showed a positive reaction in 129 ampicillin-resistant clinical isolates. Among the 75 trimethoprim-resistant isolates studied, 52% were shown to contain Tn7 when probed with a 1 kb BamHI fragment of Tn7. Results from Southern hybridizations demonstrated that these antibiotic resistant genes had been born on plasmids in some clinical isolates.
Chang
S F
SF
Department of Microbiology, Kaohsiung Medical College, Taiwan, Republic of China.
Chang
L L
LL
Chow
T Y
TY
Wu
W J
WJ
Chang
J C
JC
D000668:Ampicillin Resistance / Q000235:genetics*; D015342:DNA Probes; D004251:DNA Transposable Elements; D006090:Gram-Negative Bacteria / Q000235:genetics; D006801:Humans; D007613:Kanamycin Resistance / Q000235:genetics*; D015995:Prevalence; D014296:Trimethoprim Resistance / Q000235:genetics*; D014552:Urinary Tract Infections / Q000382:microbiology*
D015342:DNA Probes; D004251:DNA Transposable Elements