Anisodamine antagonizes acetylcholine-induced inhibition of adrenergic neurotransmission in the canine saphenous vein.
The effect of anisodamine, an alkaloid structurally related to atropine and isolated from a Chinese herb, on adrenergic neurotransmission was studied using isolated canine saphenous veins. Helical strips of vein were incubated in modified physiological salt solution containing L-(7- 3H)-norepinephrine (3 x 10(-7) mol/L) for two hours and then mounted for isometric tension recording and superfusion. Increases in isometric tension and efflux of total tritiated compounds and 3H-norepinephrine evoked by electrical stimulation were inhibited by acetylcholine (10(-6) mol/L). This effect of acetylcholine was antagonized to comparable levels by anisodamine (10(-6) mol/L) or atropine (10(-6) mol/L). The results demonstrate that anisodamine blocks prejunctional muscarinic receptors.
Guo
H
H
Institute of Basic Medical Sciences, CAMS, Beijing.
Lorenz
R R
RR
Vanhoutte
P M
PM
D000109:Acetylcholine / Q000037:antagonists & inhibitors*; D000818:Animals; D001285:Atropine / Q000494:pharmacology; D004285:Dogs; D066298:In Vitro Techniques; D009119:Muscle Contraction / Q000187:drug effects; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000294:innervation*; D009638:Norepinephrine / Q000494:pharmacology; D010276:Parasympatholytics / Q000494:pharmacology*; D011976:Receptors, Muscarinic / Q000187:drug effects; D012501:Saphenous Vein; D012991:Solanaceous Alkaloids / Q000494:pharmacology*; D009435:Synaptic Transmission / Q000187:drug effects*
D010276:Parasympatholytics; D011976:Receptors, Muscarinic; D012991:Solanaceous Alkaloids; C003922:anisodamine; D001285:Atropine; D000109:Acetylcholine; D009638:Norepinephrine