Early activation of the proto-oncogene c-fgr during Epstein-Barr virus immortalization.
In an attempt to clarify the chronological relationships between Epstein-Barr virus (EBV) infection, B cell immortalization and c-fgr activation, we evaluated for the presence of EBV-determined nuclear antigen (EBNA), cellular DNA synthesis and expression of c-fgr-specific RNA following infection of human peripheral blood lymphocytes with B95-8 EBV. High expression of c-fgr was observed prior to EBNA detection and cellular DNA synthesis in EBV-infected cells. These results suggest that activation of c-fgr is an essential event during the early phase of EBV immortalization.
Okano
M
M
Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha 68198-3135.
Davis
J R
JR
Brichacek
B
B
Yasuda
N
N
Purtilo
D T
DT
D000956:Antigens, Viral / Q000032:analysis; D001402:B-Lymphocytes / Q000378:metabolism / Q000382:microbiology*; D004261:DNA Replication; D019309:Epstein-Barr Virus Nuclear Antigens; D005786:Gene Expression Regulation; D004854:Herpesvirus 4, Human / Q000276:immunology; D006801:Humans; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins / Q000378:metabolism*; D019061:src-Family Kinases
D000956:Antigens, Viral; D019309:Epstein-Barr Virus Nuclear Antigens; C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins; C053102:proto-oncogene proteins c-fgr; D019061:src-Family Kinases