title: Coexpression of the receptor-associated protein gephyrin changes the ligand binding affinities of alpha 2 glycine receptors.
abstract: The inhibitory glycine receptor (GlyR) is a ligand-gated chloride channel protein, whose ligand binding alpha subunit occurs in several isoforms in the mammalian central nervous system. Here we show that coexpression of the GlyR-associated protein gephyrin changes the agonist and antagonist binding affinities of GlyRs generated by alpha 2 subunit expression in 293 kidney cells. Thus, a receptor-associated protein modifies the functional properties of a neurotransmitter receptor. This may contribute to an optimization of the postsynaptic neurotransmitter response.
authors.affiliation: Zentrum für Molekulare Biologie, Universität Heidelberg, Germany.
authors.forename: T
authors.initials: T
authors.lastname: Takagi
authors.forename: I
authors.initials: I
authors.lastname: Pribilla
authors.forename: J
authors.initials: J
authors.lastname: Kirsch
authors.forename: H
authors.initials: H
authors.lastname: Betz
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D015870:Gene Expression; D005998:Glycine / Q000378:metabolism*; D006801:Humans; D008024:Ligands; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D018009:Receptors, Glycine; D017981:Receptors, Neurotransmitter / Q000378:metabolism*; D013331:Strychnine / Q000494:pharmacology
chemical_list: D002352:Carrier Proteins; D008024:Ligands; D008565:Membrane Proteins; D018009:Receptors, Glycine; D017981:Receptors, Neurotransmitter; C073441:gephyrin; D013331:Strychnine; D005998:Glycine