The radioprotective effects of WR-2721 on radiation-induced goblet cell loss.
We assessed the efficacy of the radioprotective effects of WR-2721 in radiation-induced goblet cell loss. Fourteen female New Zealand white rabbits were divided into radioprotected, radiounprotected, and control groups. Ten rabbits received 5,000 rad of beta irradiation to the temporal conjunctiva of both eyes. The right eyes were pretreated with topical applications of WR-2721 30 minutes and 1 minute before irradiation. The left eyes received irradiation only. Four additional rabbits received neither irradiation nor pretreatment with WR-2721. After a 2-month waiting period, each animal was killed and goblet cell densities per high-power field (GCD/HPF) were determined over the areas in question and compared using paired sample t tests. The mean GCD/HPF for the radioprotected, unprotected, and control groups were 10.93, 4.68, 23.15 (right eye), and 26.25 (left eye). Paired sample t tests showed a significant radioprotective effect when comparing WR-2721-treated animals with those receiving radiation only (p less than 0.003). Independent sample t tests showed significant goblet cell loss in both the radioprotected (p less than 0.018) and radiounprotected eyes (p less than 0) when compared with control groups. These data suggest a radioprotective effect of WR-2721 in radiation-induced goblet cell loss.
Stonecipher
K G
KG
Department of Ophthalmology, Tulane University School of Medicine, New Orleans, LA 70118.
Shin
G
G
Pohlod
M
M
Tepper
D
D
Karcioglu
Z A
ZA
Carey
B J
BJ
Haik
B G
BG
D004999:Amifostine / Q000494:pharmacology*; D000818:Animals; D002452:Cell Count; D003228:Conjunctiva / Q000166:cytology* / Q000528:radiation effects*; D005260:Female; D011817:Rabbits
D004999:Amifostine