[T-cell receptor repertoire in tumor infiltrating lymphocytes within malignant brain tumors].
Expression of T cell receptor (TCR) V alpha and V beta genes in tumor infiltrating lymphocytes (TILs) within human malignant brain tumor was examined. Primers for 18 different human TCR V alpha and 21 V beta families were used to analyze TCRV-(D)-J-C gene rearrangements in TILs in 8 human malignant glioma specimens obtained at surgery. Using the polymerase chain reaction (PCR) method, we detected limited TCR variable region, V alpha gene expression in malignant glial tumors and also V alpha 7 and V alpha 12 TCR genes were preferentially expressed. Usage of TCR V beta gene was not as restricted as in TCR V alpha. These TILs expressing a limited repertoire of TCRs might be isolated, expanded, and used therapeutically for treatment of malignant brain tumors.
Nitta
T
T
Department of Neurology and Neurological Science, Stanford University School of Medicine.
Ikeda
M
M
Cogen
P
P
Steinman
L
L
Sato
K
K
D000293:Adolescent; D000328:Adult; D001483:Base Sequence; D001932:Brain Neoplasms / Q000235:genetics / Q000276:immunology*; D002675:Child, Preschool; D005260:Female; D015870:Gene Expression; D015332:Gene Rearrangement, alpha-Chain T-Cell Antigen Receptor; D015333:Gene Rearrangement, beta-Chain T-Cell Antigen Receptor; D006801:Humans; D007223:Infant; D016246:Lymphocytes, Tumor-Infiltrating / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics / Q000378:metabolism*
D015345:Oligonucleotide Probes; D016693:Receptors, Antigen, T-Cell, alpha-beta