Mitochondrial production of oxygen free radicals in the heart muscle during the life span of the rat: peak at middle age.
Mitochondria extracted from Wistar rat hearts at 3, 14-18 and 24 months of age showed no change in state 3-mitochondrial respiration measured in the presence of glutamate or succinate. Again no changes were found in the SMP-O2- production at the level of the rotenone-inhibited region, whilst at the level of the antimycin-inhibited region there was a marked increase in O2- production in the group of 14-18-month-old rats. In the same age period, the production of mitochondrial H2O2 supported by glutamate or succinate and the level of GSSG increased in comparison to the young group, accompanied by a decrease in the GSH level. Mitochondrial TBARS levels did not change during a life span, while a progressive accumulation in the mitochondrial lipofuscin content with age was measured.
Guarnieri
C
C
Department of Biochemistry, University of Bologna, Italy.
Muscari
C
C
Caldarera
C M
CM
D000375:Aging / Q000378:metabolism*; D000818:Animals; D005609:Free Radicals; D005978:Glutathione / Q000031:analogs & derivatives / Q000378:metabolism; D019803:Glutathione Disulfide; D006861:Hydrogen Peroxide / Q000378:metabolism*; D008062:Lipofuscin / Q000378:metabolism; D008929:Mitochondria, Heart / Q000378:metabolism*; D010101:Oxygen Consumption; D051381:Rats; D017208:Rats, Wistar; D013481:Superoxides / Q000378:metabolism*; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis / Q000378:metabolism
D005609:Free Radicals; D008062:Lipofuscin; D017392:Thiobarbituric Acid Reactive Substances; D013481:Superoxides; D006861:Hydrogen Peroxide; D005978:Glutathione; D019803:Glutathione Disulfide