title: Secretion of the Escherichia coli outer membrane proteins OmpA and OmpF in Bacillus subtilis is blocked at an early intracellular step.
abstract: When the genes coding for the outer membrane (OM) proteins OmpA and OmpF of Escherichia coli are fused to a signal sequence of a bacillar exoenzyme and expressed in Bacillus subtilis they remain cell-bound and the signal sequence is not cleaved. To identify the step of arrest in the export of these proteins we studied their accessibility to protease applied to intact protoplasts; they remained resistant indicating fully intracellular localization. Both proteins appeared associated with the cell membranes in sedimentation and flotation centrifugation experiments. However, OmpA and OmpF proteins synthesized in B. subtilis without a signal sequence were similarly associated with membranes in centrifugation experiments whereas electron microscopy showed the presence of intracytoplasmic inclusion bodies not obviously attached to the cytoplasmic membrane. We conclude that OmpA and OmpF proteins even when provided with a functional signal sequence do not enter the export pathway in B. subtilis, probably owing to lack of a specific export component in B. subtilis.
authors.affiliation: Department of Molecular Bacteriology, National Public Health Institute, Helsinki, Finland.
authors.forename: R
authors.initials: R
authors.lastname: Puohiniemi
authors.forename: M
authors.initials: M
authors.lastname: Simonen
authors.forename: S
authors.initials: S
authors.lastname: Muttilainen
authors.forename: J P
authors.initials: JP
authors.lastname: Himanen
authors.forename: M
authors.initials: M
authors.lastname: Sarvas
mesh_terms: D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000235:genetics* / Q000378:metabolism / Q000648:ultrastructure; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics*; D005227:Fatty Acids / Q000378:metabolism; D008969:Molecular Sequence Data; D010957:Plasmids; D018272:Porins; D011499:Protein Processing, Post-Translational; D021382:Protein Sorting Signals / Q000235:genetics / Q000378:metabolism; D011523:Protoplasts / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D014357:Trypsin / Q000378:metabolism
chemical_list: D001425:Bacterial Outer Membrane Proteins; D005227:Fatty Acids; D018272:Porins; D021382:Protein Sorting Signals; D011993:Recombinant Fusion Proteins; D014357:Trypsin