Increased sensitivity for detection of human cytomegalovirus in urine by removal of inhibitors for the polymerase chain reaction.
The presence of inhibitors in urine interferes with the enzymatic reaction of the polymerase chain reaction (PCR) for detection of human cytomegalovirus (HCMV). To remove inhibitors, HCMV virions in urine were precipitated with polyethylene glycol, or DNA was extracted from urine by the use of glass powder and subjected to PCR followed by Southern blot hybridization with alkaline phosphatase-linked oligonucleotide probes. These simple, rapid methods increased significantly the sensitivity of PCR for detection of HCMV in urine.
Yamaguchi
Y
Y
Department of Cell Regulation, Tokyo Medical and Dental University, Japan.
Hironaka
T
T
Kajiwara
M
M
Tateno
E
E
Kita
H
H
Hirai
K
K
D001483:Base Sequence; D011232:Chemical Precipitation; D003587:Cytomegalovirus / Q000302:isolation & purification*; D004279:DNA, Viral / Q000652:urine; D005898:Glass; D006801:Humans; D008969:Molecular Sequence Data; D011092:Polyethylene Glycols; D016133:Polymerase Chain Reaction / Q000379:methods / Q000592:standards; D011208:Powders; D014556:Urine / Q000737:chemistry / Q000382:microbiology*
D004279:DNA, Viral; D011208:Powders; D011092:Polyethylene Glycols