A study of chromosome 6 allele loss in human colorectal carcinomas.
Matched normal/tumor DNA pairs from patients with sporadic and hereditary (FAP = familial adenomatous polyposis) colorectal carcinoma were examined for tumor-specific allele loss on chromosome 6 using cDNA probes for the avian myeloblastosis viral oncogene homologue (MYB on 6q22-q23), the estrogen receptor (ESR on 6q24-q27), and for the alpha polypeptide of human chorionic gonadotropin (CGA on 6q14-q21). No chromosome 6 allele loss was observed at these gene loci among 22 colorectal carcinomas examined, although such losses were relatively frequent (37.5% of informative individuals) at the D17S28 locus on chromosome 17. These results are consistent with karyological studies and indicate that chromosome 6 allele loss from colorectal carcinomas may occur less frequently than previously reported.
Wildrick
D M
DM
Creighton Cancer Center, Creighton University School of Medicine, Omaha, Nebraska 68178.
Alfaro
S R
SR
Gope
R
R
Boman
B M
BM
D011125:Adenomatous Polyposis Coli / Q000235:genetics* / Q000473:pathology / Q000601:surgery; D000483:Alleles; D015139:Blotting, Southern; D006063:Chorionic Gonadotropin / Q000235:genetics; D002872:Chromosome Deletion; D002874:Chromosome Mapping; D002896:Chromosomes, Human, Pair 6; D003110:Colonic Neoplasms / Q000235:genetics* / Q000473:pathology / Q000601:surgery; D015342:DNA Probes; D004273:DNA, Neoplasm / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D009857:Oncogenes; D011960:Receptors, Estrogen / Q000235:genetics; D012004:Rectal Neoplasms / Q000235:genetics* / Q000473:pathology / Q000601:surgery
D006063:Chorionic Gonadotropin; D015342:DNA Probes; D004273:DNA, Neoplasm; D011960:Receptors, Estrogen