Monoclonal antibodies for the identification of herpesvirus simiae (B virus).
To differentiate between B virus and HSV isolates from monkeys and man monoclonal antibodies (mabs) were produced to herpesvirus simiae (B virus) and herpes simplex type 1 and 2 (HSV-1 and HSV-2). Mabs were tested by indirect immunofluorescence (IFAT) for reactivity against herpesviruses from Asiatic monkeys (B virus), African monkeys (SA 8 virus), and man (HSV-1, HSV-2, varicella-zoster virus, cytomegalovirus, and Epstein-Barr virus). Mabs could be divided into groups A-E displaying specific reactivity for B virus (A); reactivity with both B virus and SA 8 but not HSV (B); reactivity with B virus, SA 8 virus and HSV strains (C); specific reactivity with HSV-1 (D); and specific reactivity with HSV-2 (E). Two of the B virus specific mabs were able to differentiate between cynomolgus and rhesus strains of B virus. None of the mabs reacted with human varicella-zoster virus, cytomegalovirus, or Epstein-Barr virus. A panel of mabs for the unequivocal identification of B virus isolates from monkey or man is proposed.
Cropper
L M
LM
Virus Reference Laboratory, Central Public Health Laboratory, London, U.K.
Lees
D N
DN
Patt
R
R
Sharp
I R
IR
Brown
D
D
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D002522:Chlorocebus aethiops; D005455:Fluorescent Antibody Technique; D005720:Gamma Rays; D006566:Herpesviridae Infections / Q000175:diagnosis; D006568:Herpesvirus 1, Cercopithecine / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans; D008252:Macaca fascicularis; D008253:Macaca mulatta; D051379:Mice; D008807:Mice, Inbred BALB C; D009500:Neutralization Tests; D010215:Papio; D014709:Vero Cells
D000911:Antibodies, Monoclonal