The cAMP-binding ectoprotein from Saccharomyces cerevisiae is membrane-anchored by glycosyl-phosphatidylinositol.
Saccharomyces cerevisiae contains an amphiphilic cAMP-binding glycoprotein at the outer face of the plasma membrane (M(r) = 54,000). It is converted to a hydrophilic form by treatment with glycosyl-phosphatidylinositol-specific phospholipases C and D (GPI-PLC/D), suggesting membrane anchorage by a covalently bound glycolipid. Determination of the constituents of the purified anchor by gas-liquid chromatography and amino acid analysis reveals the presence of glycerol, myo-inositol, glucosamine, galactose, mannose, ethanolamine, and asparagine (as the carboxyl-terminal amino acid of the Pronase-digested protein to which the anchor is attached). Complementary results are obtained by metabolic labeling, indicating that fatty acids and phosphorus are additional anchor constituents. The phosphorus is resistant to alkaline phosphatase, whereas approximately half is lost from the protein after treatment with GPI-PLD or nitrous acid, and all is removed by aqueous HF indicating the presence of two phosphodiester bonds. Inhibition of N-glycosylation by tunicamycin or removal of protein-bound glycan chains by N-glycanase or Pronase does not abolish radiolabeling of the anchor structure by any of the above compounds. Analysis of the products obtained after sequential enzymic and chemical degradation of the anchor agrees with the arrangement of constituents in GPIs from higher eucaryotes. Evidence for anchorage of the yeast cAMP-binding protein by a GPI anchor is strengthened additionally by the reactivity of the GPI-PLC-cleaved anchor with antibodies directed against the cross-reacting determinant of trypanosomal variant surface glycoproteins.
Müller
G
G
Hoechst Aktiengesellschaft/Frankfurt am Main, Federal Republic of Germany.
Schubert
K
K
Fiedler
F
F
Bandlow
W
W
D000581:Amidohydrolases; D002241:Carbohydrates / Q000032:analysis; D002352:Carrier Proteins / Q000302:isolation & purification / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D002855:Chromatography, Thin Layer; D000242:Cyclic AMP / Q000378:metabolism*; D002373:Cyclic AMP Receptor Protein; D006023:Glycoproteins / Q000302:isolation & purification / Q000378:metabolism*; D017261:Glycosylphosphatidylinositols / Q000378:metabolism*; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase; D010710:Phosphates / Q000378:metabolism; D010739:Phospholipase D / Q000378:metabolism; D011402:Pronase; D011953:Receptors, Cyclic AMP / Q000302:isolation & purification / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D014415:Tunicamycin / Q000494:pharmacology; D010738:Type C Phospholipases / Q000378:metabolism
D002241:Carbohydrates; D002352:Carrier Proteins; D002373:Cyclic AMP Receptor Protein; D006023:Glycoproteins; D017261:Glycosylphosphatidylinositols; D010710:Phosphates; D011953:Receptors, Cyclic AMP; D014415:Tunicamycin; D000242:Cyclic AMP; D010738:Type C Phospholipases; D010739:Phospholipase D; D011402:Pronase; D000581:Amidohydrolases; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase