[The use of DNA hybridization in situ for identifying chromosomal rearrangements in the karyotyping of cell lines].
A complex karyological analysis of three human cell lines (PLC-PRF-5, MT-4 and U937) was carried out that involved traditional methods of G-, C-banding and silver staining, in addition to the in situ hybridization technique using 4 alpha-satellite DNA probes: DNA specific for centromeric regions of chromosome 11, 6, 13, and 21, and 14 and 22. The application of this additional method allowed to identify, prove or detalize the structure of 13 markers in PLC-PRF-5 cells, 1 marker in MT-4 cells, and 3 markers in U937 cells. The results show that the in situ hybridization method would be successful in cell line karyotyping for a more objective identification of some markers difficult for analysis.
Grabovskaia
I L
IL
Glukhova
L A
LA
Tsevtkova
T G
TG
Kravets
I A
IA
Mamaeva
S E
SE
Kushch
A A
AA
D006528:Carcinoma, Hepatocellular / Q000648:ultrastructure; D002460:Cell Line; D002869:Chromosome Aberrations; D002871:Chromosome Banding / Q000379:methods; D004247:DNA / Q000235:genetics*; D015342:DNA Probes; D004273:DNA, Neoplasm / Q000235:genetics*; D015497:HIV-1; D006801:Humans; D017403:In Situ Hybridization / Q000379:methods*; D007621:Karyotyping; D008113:Liver Neoplasms / Q000648:ultrastructure; D014407:Tumor Cells, Cultured; D014776:Virus Cultivation
D015342:DNA Probes; D004273:DNA, Neoplasm; D004247:DNA
Ispol'zovanie gibridizatsii DNK in situ dlia identifikatsii khromosomnykh perestroek pri kariotipirovanii kletochnykh liniĭ.