A method for attachment of peptides to a solid surface with enhanced immunoreactivity.
Another method has been developed to attach synthetic peptides to solid supports for use in enzyme immunoassays. The method is based on passively adsorbing a synthetic peptide to a solid-phase support, then further attaching more of the same peptide by means of cross-linking to the previously adsorbed peptide. This method results in highly enhanced peptide immunoreactivity compared with that obtained with standard methodologies.
Leahy
D C
DC
Baxter Diagnostics, Inc., Pandex Division, Mundelein, IL 60060-3021.
Shah
D O
DO
Todd
J A
JA
D000595:Amino Acid Sequence; D000956:Antigens, Viral / Q000276:immunology; D003432:Cross-Linking Reagents; D016174:Hepacivirus / Q000276:immunology; D006801:Humans; D007124:Immunoenzyme Techniques; D007158:Immunologic Techniques; D008280:Magnetics; D008863:Microspheres; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology; D011137:Polystyrenes; D013499:Surface Properties
D000956:Antigens, Viral; D003432:Cross-Linking Reagents; D010455:Peptides; D011137:Polystyrenes