Angiotensin converting enzymes from urine of treated and untreated essential mild hypertensive patients (EHP) with diuretic: partial purification and characterization.
Urine of untreated EHP was eluted, on a ion-exchange chromatography, in two protein peaks with ACE activity, at 0.7 mS (BI) and 1.25 mS (BII), while urine of treated EHP, was eluted only in one peak with ACE activity (0.7 mS). BI (Mr, 88 kDa) and BII (Mr, 61 kDa) convert AI to AII, hydrolyze bradikinin, are inhibited by captopril, EDTA and metal ions.
Alves
K B
KB
Department of Biochemistry, Escola Paulista de Medicina, SP, Brasil.
Casarini
D E
DE
da Costa
R H
RH
Plavnik
F L
FL
Portela
J E
JE
Marson
O
O
D000328:Adult; D001794:Blood Pressure; D002752:Chlorthalidone / Q000627:therapeutic use*; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D006973:Hypertension / Q000188:drug therapy* / Q000201:enzymology* / Q000652:urine; D007700:Kinetics; D008297:Male; D008875:Middle Aged; D008970:Molecular Weight; D007703:Peptidyl-Dipeptidase A / Q000302:isolation & purification / Q000652:urine*; D012016:Reference Values; D013997:Time Factors
D007703:Peptidyl-Dipeptidase A; D002752:Chlorthalidone