title: Frequent oropharyngeal shedding of Epstein-Barr virus in homosexual men during early HIV infection.
abstract: OBJECTIVE
  To determine the frequency of Epstein-Barr virus (EBV) oropharyngeal shedding during HIV infection in homosexual men in the Multicenter AIDS Cohort Study.
  
  
  METHODS
  The cohort consisted of 210 men who were HIV-seronegative at their baseline study visit, 39 of whom seroconverted to HIV at a later date, and 73 asymptomatic and mildly symptomatic men with HIV infection of indeterminate duration.
  
  
  METHODS
  EBV in throat washings was detected by transformation of newborn cord blood lymphocytes.
  
  
  RESULTS
  EBV was isolated from 49% (35 out of 71) of the HIV-seropositive and 16% (33 out of 204) of the HIV-seronegative homosexual men tested at their baseline visit. Elevated EBV shedding frequency was noted 6 months before, as well as during the first HIV-seropositive clinic visit, in the men who seroconverted to HIV. Seronegative men who shed EBV at their baseline visit seroconverted to HIV within a shorter period than did non-shedders during 5 years of follow-up. Shedding of EBV was not significantly associated with either abnormal T-cell numbers, clinical symptoms or risk for development of AIDS.
  
  
  CONCLUSIONS
  There is an increased rate of EBV shedding in HIV-seropositive homosexual men that occurs very early in the course of HIV infection.
authors.affiliation: Department of Infectious Diseases and Microbiology, University of Pittsburgh Graduate School of Public Health, PA 15261.
authors.forename: J
authors.initials: J
authors.lastname: Ferbas
authors.forename: M A
authors.initials: MA
authors.lastname: Rahman
authors.forename: L A
authors.initials: LA
authors.lastname: Kingsley
authors.forename: J A
authors.initials: JA
authors.lastname: Armstrong
authors.forename: M
authors.initials: M
authors.lastname: Ho
authors.forename: S Y
authors.initials: SY
authors.lastname: Zhou
authors.forename: C R
authors.initials: CR
authors.lastname: Rinaldo
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000209:etiology / Q000382:microbiology; D000293:Adolescent; D000328:Adult; D000368:Aged; D015658:HIV Infections / Q000209:etiology / Q000382:microbiology*; D006679:HIV Seropositivity / Q000382:microbiology; D004854:Herpesvirus 4, Human / Q000302:isolation & purification* / Q000472:pathogenicity; D006716:Homosexuality; D006801:Humans; D008297:Male; D008875:Middle Aged; D009960:Oropharynx / Q000382:microbiology*; D012725:Sexual Behavior; D013997:Time Factors