title: Changes in cAMP phosphodiesterase activity and cAMP concentration during mouse preimplantation development.
abstract: Cyclic nucleotide phosphodiesterase (PDE) activity and cAMP amounts were measured in mouse preimplantation embryos at the 1-cell, 2-cell, 8-cell/morula, and mid-blastocyst stages. PDE activity remained constant between the 1-cell and 2-cell stages. It decreased by the 8-cell stage and continued to decrease by the mid blastocyst stage to about 14% of the 1- and 2-cell values. By contrast, cAMP amounts remained essentially constant at 0.05 fmole/embryo (0.3 microM) from the 1-cell to the blastocyst stage and increased to 0.175 fmole in the fully expanded blastocyst that was close to hatching. Measurements of embryo volume indicated that intracellular volume remained essentially constant up to the blastocyst stage. The morphological changes in cell shape that accompany differentiation of the trophectoderm and that are coupled with blastocoel expansion decreased the intracellular volume. This decrease resulted in an increase in the cAMP concentration to about 0.4 microM by the mid-blastocyst stage. Previous studies indicate that either cAMP or TGF-alpha/EGF can stimulate the rate of blastocoel expansion. Although TGF-alpha/EGF can elevate cAMP levels in other cell types, TGF-alpha, at a concentration that maximally stimulates the rate of blastocoel expansion, did not elevate cAMP in blastocysts. Thus, it was unlikely that elevation of cAMP is the mechanism by which TGF-alpha stimulates the rate of blastocoel expansion.
authors.affiliation: Department of Biology, University of Pennsylvania, Philadelphia 19104-6018.
authors.forename: A
authors.initials: A
authors.lastname: Dardik
authors.forename: R M
authors.initials: RM
authors.lastname: Schultz
mesh_terms: D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000378:metabolism*; D000818:Animals; D001755:Blastocyst / Q000201:enzymology / Q000378:metabolism*; D046508:Culture Techniques; D000242:Cyclic AMP / Q000378:metabolism*; D005260:Female; D051379:Mice; D011247:Pregnancy; D016211:Transforming Growth Factor alpha / Q000502:physiology
chemical_list: D016211:Transforming Growth Factor alpha; D000242:Cyclic AMP; D015105:3',5'-Cyclic-AMP Phosphodiesterases