title: Insulin and insulin-like growth factor I in follicular fluid after induction of ovulation in women undergoing in vitro fertilization.
abstract: This study was undertaken to evaluate the relationship between concentrations of insulin and insulin-like growth factor I (IGF-I) in follicular fluid and fertilization and cleavage of human oocytes fertilized in vitro. The concentration of oestradiol, progesterone, luteinizing hormone, follicle-stimulating hormone, testosterone, insulin and IGF-I was determined in 36 follicular fluids, free of visible blood contamination and containing mature oocyte-corona-cumulus complexes, obtained from 12 women undergoing in vitro fertilization. Follicular development was induced by clomiphene citrate and human menopausal gonadotrophin, and follicular aspiration was performed 35 h after an ovulatory dose of human chorionic gonadotrophin. Concentrations of IGF-I were significantly higher in follicular fluids associated with mature oocytes that fertilized and cleaved, than in follicular fluid associated with mature oocytes that did not fertilize (P < 0.001). There was no difference in the concentration of insulin between follicular fluids from which fertilized oocytes were obtained and those with oocytes that remained unfertilized. No significant correlations were found between rates of embryo cleavage, concentrations of insulin and IGF-I. Multiple linear regression analysis demonstrated that the concentrations of IGF-I in follicular fluid were predicted statistically by a negative regression coefficient for the concentration of testosterone, and by a positive regression coefficient for the concentration of progesterone in follicular fluid. No candidate variable was included in the model to predict concentrations of insulin. These data suggest an important role for IGF-I in the mature follicle.
authors.affiliation: Department of Obstetrics and Gynaecology, Virgen de las Nieves General Hospital, Granada, Spain.
authors.forename: P
authors.initials: P
authors.lastname: Jimena
authors.forename: J A
authors.initials: JA
authors.lastname: Castilla
authors.forename: F
authors.initials: F
authors.lastname: Peran
authors.forename: R
authors.initials: R
authors.lastname: Molina
authors.forename: J P
authors.initials: JP
authors.lastname: Ramirez
authors.forename: M
authors.initials: M
authors.lastname: Acebal
authors.forename: F
authors.initials: F
authors.lastname: Vergara
authors.forename: A
authors.initials: A
authors.lastname: Herruzo
mesh_terms: D000328:Adult; D000704:Analysis of Variance; D002970:Cleavage Stage, Ovum / Q000378:metabolism; D004958:Estradiol / Q000032:analysis; D005260:Female; D005306:Fertilization / Q000502:physiology; D005307:Fertilization in Vitro; D005640:Follicle Stimulating Hormone / Q000032:analysis; D015571:Follicular Fluid / Q000737:chemistry*; D006801:Humans; D007328:Insulin / Q000032:analysis*; D007334:Insulin-Like Growth Factor I / Q000032:analysis*; D008183:Luteal Phase; D007986:Luteinizing Hormone / Q000032:analysis; D010062:Ovulation Induction; D011374:Progesterone / Q000032:analysis; D012044:Regression Analysis; D013739:Testosterone / Q000032:analysis
chemical_list: D007328:Insulin; D013739:Testosterone; D011374:Progesterone; D004958:Estradiol; D007334:Insulin-Like Growth Factor I; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone