Generation of cytotoxic monoclonals against AK-5 histiocytoma: conjugation with daunomycin and use in chemotherapy.
We have successfully generated cytotoxic monoclonal antibodies against a rat histiocytoma, AK-5. Monoclonal antibodies obtained after fusing immunized rat splenocytes with SP2/0 myeloma, were cytotoxic to AK-5 cells in the presence of complement. These monoclonals were highly specific and did not show any cross reactivity with normal cells and ascitic tumors such as Zajdela ascites hepatoma or Meth A. One of the antibodies was conjugated to daunomycin and used in the chemotherapeutic treatment. Total regression of AK-5 histiocytoma was obtained after injection of daunomycin-MAb conjugate into tumor bearing animals suggesting the specific targeting of the antineoplastic drug to the tumor. The histology of the tumor sections showed extensive necrosis of the tumors after treatment of the animals with drug-MAb conjugate.
Khar
A
A
Centre for Cellular & Molecular Biology, Hyderabad, India.
Varalakshmi
C
C
Pardhasaradhi
B V
BV
Ali
A M
AM
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology; D002460:Cell Line; D003429:Cross Reactions; D003630:Daunorubicin / Q000008:administration & dosage* / Q000627:therapeutic use; D004337:Drug Carriers; D018219:Histiocytoma, Benign Fibrous / Q000188:drug therapy / Q000276:immunology* / Q000473:pathology; D006825:Hybridomas / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D009368:Neoplasm Transplantation; D051381:Rats; D011919:Rats, Inbred Strains
D000911:Antibodies, Monoclonal; D004337:Drug Carriers; D003630:Daunorubicin