Analysis of enrofloxacin and its metabolite ciprofloxacin in bovine and porcine muscle by high-performance liquid chromatography following cation exchange clean-up.
A simple and rapid method of analysis for the trace residue determination of enrofloxacin and its metabolite ciprofloxacin has been developed. Clean-up of the samples is by cation exchange solid phase extraction (SPE) and determination made by high-performance liquid chromatography using a base-deactivated column and fluorescence detection. The method has been validated for the determination of residues in bovine and porcine muscle tissue and bacon. Recoveries at the 0.010 mg kg-1 level for enrofloaxacin and ciprofloxacin respectively were 90%, 75% in bovine muscle, 75%, 54% in porcine muscle and 81%, 63% in bacon. Determination to the 0.001 mg kg-1 level in bovine muscle and to the 0.002 mg kg-1 level in porcine muscle and bacon was also carried out. The method has been used as a quantitative screening procedure.
Tarbin
J A
JA
Ministry of Agriculture, Fisheries and Food, Food Science Laboratory, Colney, Norwich, UK.
Tyler
D J
DJ
Shearer
G
G
D000818:Animals; D000890:Anti-Infective Agents / Q000032:analysis*; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D002939:Ciprofloxacin / Q000032:analysis*; D000077422:Enrofloxacin; D024841:Fluoroquinolones; D015394:Molecular Structure; D009132:Muscles / Q000737:chemistry*; D015363:Quinolones / Q000032:analysis*; D015203:Reproducibility of Results; D013552:Swine
D000890:Anti-Infective Agents; D024841:Fluoroquinolones; D015363:Quinolones; D000077422:Enrofloxacin; D002939:Ciprofloxacin