Neutralizing mechanisms of two human monoclonal antibodies against human cytomegalovirus glycoprotein 130/55.
The neutralization of human cytomegalovirus (HCMV) after adsorption to the cell surface at 4 degrees C was studied using two neutralizing monoclonal antibodies (C-23 and C-41) recognizing glycoprotein 130/55. HCMV adsorbed to cells was neutralized by C-23 (complement-independent), but not by C-41 (complement-dependent). Furthermore, the virus remained sensitive to C-23 for 120 min after shifting up from 4 degrees C to 37 degrees C, suggesting that C-23 might block an early stage of virus penetration into cells, and also that transition from virus attachment to virus penetration might be quite slow. The cell-to-cell infection of HCMV was also blocked only by C-23, and not by C-41. On the basis of the results presented here, we suggest that C-41 blocks the attachment of virus to the cell surface whereas C-23 prevents the penetration of virus into the cell.
Ohizumi
Y
Y
Virus Research Center, Sendai National Hospital, Japan.
Suzuki
H
H
Matsumoto
Y
Y
Masuho
Y
Y
Numazaki
Y
Y
D000327:Adsorption; D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral / Q000276:immunology* / Q000494:pharmacology; D002462:Cell Membrane / Q000382:microbiology; D002478:Cells, Cultured; D003080:Cold Temperature; D003165:Complement System Proteins / Q000276:immunology; D003587:Cytomegalovirus / Q000276:immunology*; D005347:Fibroblasts; D006358:Hot Temperature; D006801:Humans; D009500:Neutralization Tests; D014759:Viral Envelope Proteins / Q000276:immunology*; D014779:Virus Replication / Q000187:drug effects
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D014759:Viral Envelope Proteins; C040495:glycoprotein B, Simplexvirus; D003165:Complement System Proteins