title: The pathway of arginine catabolism in Giardia intestinalis.
abstract: In Giardia intestinalis, arginine is catabolised by the arginine dihydrolase pathway. The enzymes of the pathway (arginine deiminase, ornithine transcarbamoylase and carbamate kinase) were investigated and their basic kinetic parameters determined. The specific activity of arginine deiminase was 270 +/- 23 nmol min-1 (mg protein)-1; ornithine transcarbamoylase, in the direction of citrulline utilisation 170 +/- 22 nmol min-1 (mg protein)-1, and in the direction of ornithine utilisation 2100 +/- 100 nmol min-1 (mg protein)-1; and carbamate kinase 2100 +/- 400 nmol min-1 (mg protein)-1. The activities of these enzymes are between 10 and 250 fold greater than those reported for the enzymes in Trichomonas vaginalis, the only other parasite in which the arginine dihydrolase pathway has been reported. The flux through the pathway in G. intestinalis, as determined by the liberation of 14CO2 from 1 mM [14C-guanidino]arginine was 30 nmol min-1 (mg protein)-1. This flux was not affected by valinomycin (0.1 microM), nigericin (3 microM), azide (5 mM) or cyanide (1 mM). The flux was only marginally affected by glucose up to 10 mM concentration. Conversely, the flux through glucose metabolism, as determined by the release of 14CO2 from 1 mM [1-14C]glucose was only 2 nmol min-1 (mg protein)-1, and was unaffected by arginine concentrations up to 10 mM. These observations suggest that there is no direct metabolic interface between arginine and glucose catabolism.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: School of Biochemistry, University of New South Wales, Kensington, Australia.
authors.forename: P J
authors.initials: PJ
authors.lastname: Schofield
authors.forename: M R
authors.initials: MR
authors.lastname: Edwards
authors.forename: J
authors.initials: J
authors.lastname: Matthews
authors.forename: J R
authors.initials: JR
authors.lastname: Wilson
mesh_terms: D000818:Animals; D001120:Arginine / Q000378:metabolism*; D001692:Biological Transport / Q000502:physiology; D016829:Giardia lamblia / Q000378:metabolism*; D006867:Hydrolases / Q000378:metabolism; D009954:Ornithine Carbamoyltransferase / Q000378:metabolism; D010770:Phosphotransferases / Q000378:metabolism; D017851:Phosphotransferases (Carboxyl Group Acceptor)
chemical_list: D001120:Arginine; D009954:Ornithine Carbamoyltransferase; D010770:Phosphotransferases; D017851:Phosphotransferases (Carboxyl Group Acceptor); C019288:carbamate kinase; D006867:Hydrolases; C019723:arginine deiminase