title: Effect of small changes in extracellular magnesium concentration on the tone of feline mesenteric arteries: involvement of endothelium.
abstract: The aim of this study was to investigate the effect of small alterations in extracellular magnesium concentration on the tone of feline mesenteric arteries and to examine the role of endothelium in these responses. We measured isometrical tension of isolated arterial rings, placed between two stainless steel wires in a tissue chamber containing Krebs-Henseleit solution, aerated with a gas mixture containing 95% O2 and 5% CO2 at 37 degrees C. After precontraction with noradrenaline, a decrease of extracellular magnesium concentration from 1.2 mM to 1.0 and 0.8 mM resulted in sustained relaxations, whereas the elevation of extracellular magnesium from 0.8 mM to 1.2 mM caused an increase in vascular tone when endothelium was intact. The magnesium-withdrawal related dilations were absent in endothelium-denuded vessels and were inhibited by oxyhemoglobin (5 x 10(-6) M) and methylene blue (10(-5) M), suggesting the involvement of endothelium-derived relaxing factor in this vascular response. Nifedipine (5 x 10(-7) M) or dichlorobenzamil (3 x 10(-5) M), however, did not affect the magnesium-deficiency related relaxations. Therefore, in this vascular action of magnesium, nifedipine-sensitive calcium channels or the sodium- calcium antiport system are not involved. We conclude that small alterations in extracellular magnesium concentration, possibly within the physiological range, are able to modify the basal formation and release of EDRF, and thus alter arterial smooth muscle tone in this vascular bed. This endothelium- and magnesium-dependent system appears to be more sensitive than the direct smooth muscle actions of magnesium. The possible physiological and pathophysiological consequences of these observations are discussed.
authors.affiliation: Experimental Research Department, Semmelweis University Medical School, Budapest, Hungary.
authors.forename: C
authors.initials: C
authors.lastname: Szabó
authors.forename: M
authors.initials: M
authors.lastname: Faragó
authors.forename: E
authors.initials: E
authors.lastname: Dóra
mesh_terms: D000818:Animals; D002415:Cats; D004730:Endothelium, Vascular / Q000502:physiology*; D005260:Female; D007537:Isometric Contraction / Q000187:drug effects; D008274:Magnesium / Q000494:pharmacology*; D008297:Male; D008638:Mesenteric Arteries; D009126:Muscle Relaxation / Q000502:physiology; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000502:physiology*; D009569:Nitric Oxide / Q000502:physiology; D009638:Norepinephrine / Q000494:pharmacology
chemical_list: D009569:Nitric Oxide; D008274:Magnesium; D009638:Norepinephrine