[Effects of ANF and ACTH on aldosterone secretion in cultured human adrenal tissue].
We studied the effects of various concentrations of ANF and ACTH on aldosterone secretion in human normal adrenal tissue over different response times in vitro. We found that ANF 10(-8) mol/L at first stimulated but 24 h later inhibited basal aldosterone secretion. ANF 10(-8) mol/L also inhibited the ACTH-stimulated secretion of aldosterone. ACTH 10(-8) mol/L did not stimulate aldosterone secretion, while ACTH concentrations of 3 x 10(-8) and 5 x 10(-8) mol/L did. When ACTH and ANF were added simultaneously, the ANF-induced inhibition of ACTH-stimulated aldosterone secretion could be overcome by increasing the concentration of ACTH. Our results show that ANF caused a dose-dependent and time-dependent inhibition of basal and ACTH-stimulated increases in aldosterone secretion.
Sun
M
M
PUMC Hospital, Beijing.
D000311:Adrenal Glands / Q000378:metabolism*; D000324:Adrenocorticotropic Hormone / Q000494:pharmacology*; D000328:Adult; D000450:Aldosterone / Q000378:metabolism*; D009320:Atrial Natriuretic Factor / Q000494:pharmacology*; D046508:Culture Techniques; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008297:Male
D000450:Aldosterone; D009320:Atrial Natriuretic Factor; D000324:Adrenocorticotropic Hormone