title: Inhibition of lipopolysaccharide synthesis in Agrobacterium tumefaciens and Aeromonas salmonicida.
abstract: Lipopolysaccharide (LPS) synthesis was inhibited, new lipid A metabolites accumulated, and growth ceased, when the plant pathogen Agrobacterium tumefaciens and the fish pathogen Aeromonas salmonicida were treated with an antibacterial agent which specifically inhibits CTP:CMP-3-deoxy-manno-octulosonate cytidylyltransferase (CMP-KDO synthase). The new lipid A metabolites were purified by chromatography on DEAE-cellulose and chemically analysed. Metabolites isolated from both bacterial species contained glucosamine and phosphate in a 1:1 molar ratio, and 3-OH-C14:0 was the major fatty acid present (1 mol and 1.4 mol per mol glucosamine for A. tumefaciens and A. salmonicida, respectively). Inhibition of LPS synthesis by CMP-KDO synthase inhibitor had no effect on the initial kinetics of A. tumefaciens attachment to cultured carrot cells, but did inhibit cell aggregation normally induced by bacterial cellulose synthesis. Bacteria treated with inhibitor remained viable and able to synthesize protein at 15% the rate of control cells, indicating that the lack of cellulose-induced aggregation was not due to the inability of bacteria to make protein, but rather the inability to respond normally to the bacterial-plant cell interaction.
authors.affiliation: Anti-Infective Research Division, Abbott Laboratories, Abbott Park, Illinois 60064-3500.
authors.forename: R C
authors.initials: RC
authors.lastname: Goldman
authors.forename: J O
authors.initials: JO
authors.lastname: Capobianco
authors.forename: C C
authors.initials: CC
authors.lastname: Doran
authors.forename: A G
authors.initials: AG
authors.lastname: Matthysse
mesh_terms: D000333:Aeromonas / Q000378:metabolism*; D016960:Agrobacterium tumefaciens / Q000378:metabolism*; D002848:Chromatography, DEAE-Cellulose; D002849:Chromatography, Gas; D002855:Chromatography, Thin Layer; D007700:Kinetics; D008050:Lipid A / Q000032:analysis / Q000378:metabolism; D008070:Lipopolysaccharides / Q000096:biosynthesis*; D009713:Nucleotidyltransferases / Q000037:antagonists & inhibitors*; D010944:Plants / Q000378:metabolism
chemical_list: D008050:Lipid A; D008070:Lipopolysaccharides; D009713:Nucleotidyltransferases; C028984:3-deoxy-manno-octulosonate cytidyltransferase