title: A preliminary study of correlation of immuno-histological and ultrastructural characteristics of neural granuloma in leprosy patients.
abstract: With an aim to better understand the pathogenesis of nerve damage in leprosy, peripheral nerve biopsies from six untreated leprosy cases (3 BT/TT and 3 BL/LL) were studied by electronmicroscopy and immuno-histology. In addition to routine histopathology for diagnosis, infiltrating cells of granuloma were characterized after preparation of single cell suspension. The lymphocytes in the lesion were characterized by E and EAC rosetting and macrophage phagocytic system (MPS) cells were studied using histochemical markers like esterase and peroxidase. The results indicate that the lymphocyte content was significantly greater in tuberculoid neural granuloma compared to lepromatous nerves and these formed rosettes with sheep erythrocytes (E) and expressed HLA-DR antigen suggesting that they are activated T cells. Infiltrating macrophages in both the tuberculoid and lepromatous neural granuloma were esterase positive, peroxidase negative and did not form rosettes with sheep erythrocytes or EAC. Ultrathin sections of tuberculoid granuloma showed lymphocytes clearly associated to epithelioid macrophages having well developed Golgi apparatus and rough endoplasmic reticulum. Correlation of these immunological and ultrastructural characters suggests that hypersensitivity mechanisms are possibly responsible for nerve damage in tuberculoid leprosy. Ultrastructural examination of lepromatous nerves, on the other hand, showed the predominance of macrophages with large nucleus, heavily bacillated Schwann cells, and a few lymphocytes. The correlation of immuno-histological and ultrastructural characters indicates that the mechanism(s) of nerve damage in lepromatous leprosy are basically different wherein hypersensitivity appears to play a very limited role.
authors.affiliation: Central JALMA Institute for Leprosy (ICMR), Tajganj, Agra, U.P., India.
authors.forename: V
authors.initials: V
authors.lastname: Kumar
authors.forename: K
authors.initials: K
authors.lastname: Katoch
authors.forename: V M
authors.initials: VM
authors.lastname: Katoch
authors.forename: V P
authors.initials: VP
authors.lastname: Bharadwaj
mesh_terms: D000047:Academies and Institutes; D000554:Ambulatory Care Facilities; D001706:Biopsy; D006099:Granuloma / Q000276:immunology / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D007194:India; D015441:Leprosy, Tuberculoid / Q000276:immunology / Q000473:pathology*; D008214:Lymphocytes / Q000473:pathology; D008264:Macrophages / Q000473:pathology; D008854:Microscopy, Electron; D010523:Peripheral Nervous System Diseases / Q000276:immunology / Q000473:pathology*