Human guanylin: cDNA isolation, structure, and activity.
Guanylin is a mammalian peptide homologue of heat-stable enterotoxins that acts on intestinal guanylate cyclase to elicit an increase in cyclic GMP. We have isolated a cDNA encoding an apparent precursor of guanylin from a human intestinal cDNA library. The mRNA is expressed at high levels in human ileum and colon. Human guanylin stimulated increases in T84 cell cyclic GMP levels, displaced 125I-labelled heat-stable enterotoxin (STa) binding to this cell line, and stimulated increases in short-circuit current (Isc) of isolated rat proximal colonic mucosa. This peptide may play a role in regulating fluid and electrolyte absorption in human intestines.
Wiegand
R C
RC
Department of Molecular Biology, Monsanto Corporate Research, Monsanto Company, St. Louis, MO 63198.
Kato
J
J
Huang
M D
MD
Fok
K F
KF
Kachur
J F
JF
Currie
M G
MG
D000595:Amino Acid Sequence; D000818:Animals; D001427:Bacterial Toxins / Q000378:metabolism; D001483:Base Sequence; D002460:Cell Line; D002712:Chlorides / Q000378:metabolism; D003106:Colon / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology; D006152:Cyclic GMP / Q000378:metabolism; D004247:DNA / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D004553:Electric Conductivity / Q000187:drug effects; D004768:Enterotoxins / Q000378:metabolism; D029968:Escherichia coli Proteins; D005768:Gastrointestinal Hormones; D006801:Humans; D007082:Ileum / Q000378:metabolism*; D008969:Molecular Sequence Data; D045265:Natriuretic Peptides; D010452:Peptide Biosynthesis; D010455:Peptides / Q000737:chemistry / Q000235:genetics* / Q000494:pharmacology*; D051381:Rats
D001427:Bacterial Toxins; D002712:Chlorides; D004768:Enterotoxins; D029968:Escherichia coli Proteins; D005768:Gastrointestinal Hormones; D045265:Natriuretic Peptides; D010455:Peptides; C034151:heat stable toxin (E coli); C072685:guanylin; D004247:DNA; D006152:Cyclic GMP