PAb 2000 specifically recognizes the large T and small t proteins of JC virus.
A monoclonal antibody, PAb 2000, has been derived which recognizes the large T protein of JC virus (JCV), but not the corresponding proteins of the related polyomaviruses BK virus (BKV) and SV40. The epitope bound by PAb 2000 was localized to the amino-terminal 81 amino acids of this multifunctional protein. As observed previously with several monoclonal antibodies that bind a similar region of SV40 large T antigen, PAb 2000 was found to interact with the small t antigen and the denatured form of large T antigen. This monoclonal antibody recognized a subpopulation of T protein, the abundance of which varied in different species of cells transformed by JCV. The availability of PAb 2000, the first JCV T antigen-specific monoclonal antibody, will facilitate the purification and biochemical characterization of the JCV oncoproteins.
Bollag
B
B
Department of Molecular and Cell Biology, Pennsylvania State University, University Park 16802.
Frisque
R J
RJ
D000818:Animals; D000911:Antibodies, Monoclonal; D000957:Antigens, Viral, Tumor / Q000032:analysis*; D002460:Cell Line; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral; D007577:JC Virus / Q000302:isolation & purification*; D051379:Mice; D008810:Mice, Inbred C57BL; D011233:Precipitin Tests; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000032:analysis; D014764:Viral Proteins / Q000032:analysis*
D000911:Antibodies, Monoclonal; D000957:Antigens, Viral, Tumor; D011993:Recombinant Fusion Proteins; D014764:Viral Proteins