Internucleosomal chromatin degradation in myeloma and B-hybridoma cell cultures.
The activity of Ca/Mg-dependent endonuclease (CME) is strongly inhibited in myeloma X-63.Ag8.653 and B-hybridoma MLC-1c as compared with mouse splenocytes. Nevertheless, pronounced internucleosomal chromatin degradation occurs in both cell lines during long-term cultivation without passing. In isolated cell nuclei of X-63 the activation of CME, which precedes chromatin fragmentation in vivo and loss of cell viability, is revealed. The time-course of CME activation is opposite to cell proliferation and is not accompanied by alterations in enzyme quantity. The results suggest that cell death of X-63 and MLC-1c occurs via apoptosis, and involves the mechanisms controlling the activation and/or interaction of CME with chromatin.
Sokolova
I A
IA
National Research Center of Medical Genetics, Russian Academy of Medical Sciences, Moscow.
Volgin
A U
AU
Makarova
N V
NV
Volgina
V V
VV
Shishkin
S S
SS
Khodarev
N N
NN
D000818:Animals; D001402:B-Lymphocytes / Q000378:metabolism; D002453:Cell Cycle; D016923:Cell Death; D002843:Chromatin / Q000378:metabolism*; D004249:DNA Damage; D004720:Endonucleases / Q000378:metabolism; D004789:Enzyme Activation; D006825:Hybridomas / Q000378:metabolism; D066298:In Vitro Techniques; D051379:Mice; D009707:Nucleosomes / Q000378:metabolism; D010954:Plasmacytoma / Q000378:metabolism; D014407:Tumor Cells, Cultured
D002843:Chromatin; D009707:Nucleosomes; D004720:Endonucleases