Minoxidil specifically decreases the expression of lysine hydroxylase in cultured human skin fibroblasts.
The levels of lysine hydroxylase protein and the levels of the mRNAs for lysine hydroxylase and the alpha- and beta-subunits of proline 4-hydroxylase were measured in cultured human skin fibroblasts treated with 1 mM-minoxidil. The data demonstrate that minoxidil decreases the amount of lysine hydroxylase protein, this being due to a decrease in the level of lysine hydroxylase mRNA. The effect of minoxidil appears to be highly specific, as no changes were observed in the amounts of mRNAs for the alpha- and beta-subunits of proline 4-hydroxylase.
Hautala
T
T
Collagen Research Unit, University of Oulu, Finland.
Heikkinen
J
J
Kivirikko
K I
KI
Myllylä
R
R
D015152:Blotting, Northern; D015153:Blotting, Western; D002478:Cells, Cultured; D003864:Depression, Chemical; D005347:Fibroblasts / Q000187:drug effects* / Q000201:enzymology / Q000502:physiology; D015870:Gene Expression / Q000187:drug effects*; D006801:Humans; D015151:Immunoblotting; D046911:Macromolecular Substances; D008914:Minoxidil / Q000494:pharmacology*; D009693:Nucleic Acid Hybridization; D008240:Procollagen-Lysine, 2-Oxoglutarate 5-Dioxygenase / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D011393:Procollagen-Proline Dioxygenase / Q000235:genetics / Q000378:metabolism; D014176:Protein Biosynthesis / Q000187:drug effects; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
D046911:Macromolecular Substances; D012333:RNA, Messenger; D008914:Minoxidil; D011393:Procollagen-Proline Dioxygenase; D008240:Procollagen-Lysine, 2-Oxoglutarate 5-Dioxygenase