Eicosanoids content in small intestinal mucosa of children with celiac disease.
Celiac disease (CD) is characterized by diarrhea, growth retardation, and weight loss in genetically susceptible subjects on a gluten-containing diet. The exact pathogenesis of CD is still obscure, but it is considered to be immunologically mediated. We have previously shown elevated prostaglandin E2 (PGE2) and thromboxane B2 (TxB2) content in small intestinal mucosa obtained from active celiac children. In the present study, we found significantly elevated PGE2, leukotriene B4 (LTB4), and leukotrienes C4, D4, and E4 (LTC4D4E4) content in small bowel mucosa from children suffering from CD on a gluten-containing diet in comparison to control subjects. PGE2 was 25,278 +/- 7,761 vs. 4,478 +/- 426 pg/mg of protein (mean +/- SEM), respectively. LTB4 was 8,807 +/- 3,706 vs. 403 +/- 63 pg/mg of protein (mean +/- SEM), respectively. LTC4D4E4 was 15,369 +/- 4,085 vs. 2,998 +/- 279 pg/mg of protein (mean +/- SEM), respectively. We conclude that the elevated content of arachidonic acid metabolic products via cyclooxygenase and lipoxygenase pathways may contribute to the diarrhea and may be involved in the pathogenesis of mucosal injury.
Branski
D
D
Department of Pediatrics, Bikur Cholim General Hospital, Jerusalem, Israel.
Hurvitz
H
H
Halevi
A
A
Klar
A
A
Navon
P
P
Weidenfeld
J
J
D000293:Adolescent; D002446:Celiac Disease / Q000378:metabolism*; D002648:Child; D002675:Child, Preschool; D015232:Dinoprostone / Q000096:biosynthesis; D015777:Eicosanoids / Q000032:analysis*; D006801:Humans; D007223:Infant; D007413:Intestinal Mucosa / Q000378:metabolism*; D007421:Intestine, Small / Q000378:metabolism*; D007975:Leukotriene B4 / Q000096:biosynthesis; D013189:SRS-A / Q000096:biosynthesis
D015777:Eicosanoids; D013189:SRS-A; D007975:Leukotriene B4; D015232:Dinoprostone