title: Retrovirus-mediated gene transfer targeted to malignant glioma cells in murine brain.
abstract: A murine model for meningeal metastasis of malignant glioma was developed to study selective gene transfer into tumor cells and to establish a reliable means of determining the rate of tumor cell infection. A murine ecotropic retroviral vector was created in which the Escherichia coli beta-galactosidase gene served as a marker for gene expression from the integrated retrovirus. This retrovirus exhibited a high rate of infectivity in RSV-M mouse glioma cells in vitro. The recombinant retrovirus was injected directly into the cisterna magna of the mice. Staining of beta-galactosidase showed that the rate of gene integration was high in the disseminated glioma cells. These results suggest the possibility of retrovirus-mediated gene therapy for meningeal dissemination of malignant glioma.
authors.affiliation: Department of Neurosurgery, Center for Adult Diseases, Osaka.
authors.forename: M
authors.initials: M
authors.lastname: Yamada
authors.forename: K
authors.initials: K
authors.lastname: Shimizu
authors.forename: Y
authors.initials: Y
authors.lastname: Miyao
authors.forename: T
authors.initials: T
authors.lastname: Hayakawa
authors.forename: K
authors.initials: K
authors.lastname: Ikenaka
authors.forename: K
authors.initials: K
authors.lastname: Nakahira
authors.forename: K
authors.initials: K
authors.lastname: Nakajima
authors.forename: T
authors.initials: T
authors.lastname: Kagawa
authors.forename: K
authors.initials: K
authors.lastname: Mikoshiba
mesh_terms: D000818:Animals; D001483:Base Sequence; D001932:Brain Neoplasms / Q000235:genetics*; D002946:Cisterna Magna; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005798:Genes, Bacterial / Q000235:genetics*; D005822:Genetic Vectors / Q000235:genetics*; D005910:Glioma / Q000235:genetics*; D008578:Meninges; D051379:Mice; D008969:Molecular Sequence Data; D009362:Neoplasm Metastasis; D013539:Simian virus 40 / Q000235:genetics*; D014162:Transfection / Q000379:methods*; D014407:Tumor Cells, Cultured; D001616:beta-Galactosidase / Q000032:analysis / Q000235:genetics*
chemical_list: D001616:beta-Galactosidase