Protein phosphorylation is involved in the regulation of chromatin condensation during interphase.
Loci affecting the condensation state of interphase chromatin have been previously identified from analysis of suppression and enhancement of position effect variegation (PEV) in Drosophila. Here we show that Su-var(3)6 and an allelic mutant, e078, which both show suppression of PEV in the heterozygous state, have point mutations (Gly220-->Ser and Gly220-->Asp, respectively) in a protein phosphatase 1 catalytic subunit located at 87B (PP1 87B). The mutated glycine is conserved in all known protein serine/threonine phosphatases in the same gene family, and its substitution decreases PP1 activity. We conclude that protein dephosphorylation by PP1 87B regulates the condensation state of chromatin during interphase.
Dombrádi
V
V
Department of Biochemistry, The University, Dundee, Scotland, UK.
Cohen
P T
PT
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002843:Chromatin / Q000378:metabolism*; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D015342:DNA Probes; D004330:Drosophila / Q000235:genetics / Q000502:physiology*; D007399:Interphase; D007438:Introns; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D005810:Multigene Family; D009154:Mutation; D010749:Phosphoprotein Phosphatases / Q000235:genetics*; D010766:Phosphorylation; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D054645:Protein Phosphatase 1; D017384:Sequence Deletion; D017386:Sequence Homology, Amino Acid
D002843:Chromatin; D015342:DNA Probes; D046911:Macromolecular Substances; D004247:DNA; D010749:Phosphoprotein Phosphatases; D054645:Protein Phosphatase 1