title: Construction of complex directional complementary DNA libraries in SfiI.
authors.affiliation: Division of Hematologic Oncology/Lymphoma of Memorial Hospital Program in Molecular Biology, Sloan-Kettering Institute, Memorial Sloan-Kettering Cancer Center, New York, New York 10021.
authors.forename: A D
authors.initials: AD
authors.lastname: Zelenetz
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods*; D004247:DNA / Q000235:genetics*; D004262:DNA Restriction Enzymes; D004273:DNA, Neoplasm / Q000235:genetics*; D004274:DNA, Recombinant / Q000096:biosynthesis*; D004259:DNA-Directed DNA Polymerase; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics*; D015723:Gene Library; D005822:Genetic Vectors; D006801:Humans; D007202:Indicators and Reagents; D008223:Lymphoma / Q000235:genetics*; D008969:Molecular Sequence Data; D010957:Plasmids; D011116:Polynucleotide 5'-Hydroxyl-Kinase; D012334:RNA, Neoplasm / Q000235:genetics / Q000302:isolation & purification; D015183:Restriction Mapping; D013379:Substrate Specificity; D013604:T-Phages / Q000201:enzymology
chemical_list: D004273:DNA, Neoplasm; D004274:DNA, Recombinant; D007202:Indicators and Reagents; D012334:RNA, Neoplasm; D004247:DNA; D011116:Polynucleotide 5'-Hydroxyl-Kinase; D004259:DNA-Directed DNA Polymerase; D004262:DNA Restriction Enzymes; C041749:endodeoxyribonuclease SfiI; D015252:Deoxyribonucleases, Type II Site-Specific