Propagation of adenovirus types 40 and 41 in the PLC/PRF/5 primary liver carcinoma cell line.
The sensitivity of cell cultures to adenovirus types 40 and 41 (Ad40/41) was compared by means of cell culture infectious dose (ID50) assays using monolayer cultures in microtitre plates. The PLC/PRF/5 cell line derived from a primary human hepatocellular carcinoma was 100 times more sensitive to a laboratory strain of Ad41, and 10 times more sensitive to a laboratory strain of Ad40 and two Ad41 stool isolates, than Graham 293 and Chang conjunctival cells commonly used for the propagation of these viruses. In microtitre plate titration assays PLC/PRF/5 cells retained an optimal condition for longer and displayed cytopathogenic effects earlier and more clearly than the other cell lines. In contrast to previously used cells, PLC/PRF/5 cells also proved successful for the quantitation of Ad41, but not Ad40, by conventional plaque assays. The reason for the exceptional susceptibility of PLC/PRF/5 cells has not been elucidated, but the findings open attractive new doors for research on Ad40/41.
Grabow
W O
WO
Department of Medical Virology, University of Pretoria, South Africa.
Puttergill
D L
DL
Bosch
A
A
D000256:Adenoviridae / Q000502:physiology*; D006528:Carcinoma, Hepatocellular / Q000382:microbiology*; D002460:Cell Line; D003588:Cytopathogenic Effect, Viral; D006801:Humans; D007223:Infant; D008113:Liver Neoplasms / Q000382:microbiology*; D014407:Tumor Cells, Cultured; D014779:Virus Replication