Anti-human immunodeficiency virus (HIV) agents are also potent and selective inhibitors of feline immunodeficiency virus (FIV)-induced cytopathic effect: development of a new method for screening of anti-FIV substances in vitro.
The ability of several known anti-HIV substances to inhibit feline immunodeficiency virus (FIV) was tested. The results showed that FIV infection of feline T-cells was almost completely blocked in the presence of all of the agents tested. However, FIV-induced syncytium formation between a human T-cell line (MT-2 cells) and a FIV-infected feline lymphocyte cell line (3201/FIV) was inhibited only by dextran sulfate and pradimicin A. The assay used to measure syncytium inhibition was rapid and did not use potentially hazardous human immunodeficiency virus (HIV)-infected cells. The efficacy results coincided with those of HIV studies.
Tanabe-Tochikura
A
A
Department of Veterinary Pathobiology, Ohio State University, Columbus 43210.
Tochikura
T S
TS
Blakeslee
J R
JR
Olsen
R G
RG
Mathes
L E
LE
D000818:Animals; D018943:Anthracyclines; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology; D000998:Antiviral Agents / Q000494:pharmacology*; D002415:Cats; D002459:Cell Fusion / Q000187:drug effects; D002478:Cells, Cultured; D003588:Cytopathogenic Effect, Viral / Q000187:drug effects*; D016264:Dextran Sulfate / Q000494:pharmacology; D016049:Didanosine / Q000494:pharmacology; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D006678:HIV / Q000187:drug effects*; D006493:Heparin / Q000494:pharmacology; D006801:Humans; D016089:Immunodeficiency Virus, Feline / Q000187:drug effects*; D014779:Virus Replication / Q000187:drug effects; D015215:Zidovudine / Q000494:pharmacology; D000095643:Pradimicins and Benanomicins
D018943:Anthracyclines; D000903:Antibiotics, Antineoplastic; D000998:Antiviral Agents; D016264:Dextran Sulfate; D016049:Didanosine; D006493:Heparin; D015215:Zidovudine; C058036:pradimicin A; D000095643:Pradimicins and Benanomicins