[A research for screening anti-hepatitis B virus drugs with the 2.2.15 cell line].
In this paper, 43 drugs have been screened and evaluated for their ability of anti-hepatitis B virus by way of detecting in the cell culture medium the HBsAG and HBeAG secreted from the 2.2.15 cell line--a cell line derived from the Hep-G2 transfected with cloned HBV DNA. In addition, the MTT colorimetric assay is used in the process for monitoring the cytotoxic effects. The result showed that five drugs, including anti-HBV-I, anti-HBV-II, Radix Astragali Co., Rhizoma Curculiginis Co. and 518BII-A-6 are promising anti-HBV drugs.
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Department of Infectious Disease, First Teaching Hospital, Beijing Medical University.
D000998:Antiviral Agents / Q000494:pharmacology*; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000473:pathology; D004279:DNA, Viral / Q000235:genetics; D004353:Drug Evaluation, Preclinical; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D006515:Hepatitis B virus / Q000187:drug effects* / Q000235:genetics; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics / Q000473:pathology; D014162:Transfection; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000382:microbiology
D000998:Antiviral Agents; D004279:DNA, Viral; D004365:Drugs, Chinese Herbal