A murine leukemia virus derived retroviral vector with a rat VL30 packaging psi sequence.
A rat VL30 packaging Psi sequence of 168 nucleotides has been characterized. It bears little or no homology to the Murine Leukemia Virus (MLV) Psi sequence, is much smaller in size and at least as efficient for packaging. The use of this small VL30 Psi sequence in MLV derived retroviral vectors should render even more unlikely recombinations possibly generating replication competent MLV viruses. Thus, utilization of the rat VL30 Psi sequence should improve the biological safety of MLV derived vectors for human gene transfer.
Torrent
C
C
LaboRetro INSERM, Ecole Normale Supérieure de Lyon, France.
Wang
P
P
Darlix
J L
JL
D016475:3T3 Cells; D000818:Animals; D003673:Defective Viruses / Q000235:genetics*; D005814:Genes, Viral; D005822:Genetic Vectors; D006251:Harvey murine sarcoma virus / Q000235:genetics; D006367:HeLa Cells; D006801:Humans; D009052:Leukemia Virus, Murine / Q000235:genetics*; D051379:Mice; D051381:Rats / Q000235:genetics*; D012045:Regulatory Sequences, Nucleic Acid; D014162:Transfection