Binding of a cementum attachment protein to extracellular matrix components and to dental surfaces.
Cementum proteins (CP) have been shown to mediate cell attachment. Among these, a 55 kDa protein was isolated. The purpose of the present study was to assess the capacity of CP to bind to non-demineralized and demineralized root surfaces and to support cell attachment to dentin. CP were prepared by sequential extraction of bovine cementum with 25 mM EDTA, 0.5 M acetic acid followed by 4 M guanidine HCl. The latter was subjected to ion exchange chromatography on a DEAE-3SW column and eluted stepwise with a 0-0.5 M NaCl gradient. CP were labelled with 125I and the capacity of 125I-CP to bind to mineralized and partially demineralized dentin, synthetic hydroxyapatite, collagen, fibronectin and fibrillar collagen-fibronectin complex was assessed. It was found that CP bind specifically to mineralized dentin and synthetic hydroxyapatite but not to demineralized dentin. The specific binding was 60% of the total binding. SDS-PAGE analysis of the proteins bound to dentin indicated that the main bound protein had a molecular weight of 55 kDa. CP exhibited high affinity for fibronectin (kD = 1.56 x 10(-10) M) and fibronectin-collagen complex, but their binding to either molecular or fibrillar collagen was negligible. It is suggested that CP may play an important role in the attachment of cells of the periodontium to cementum extracellular matrix during homeostasis and regeneration.
Pitaru
S
S
Department of Oral Biology, Goldschleger School of Dental Medicine, Tel Aviv University, Israel.
Savion
N
N
Hekmati
H
H
Olsen
S
S
Narayanan
S A
SA
D000818:Animals; D002417:Cattle; D002448:Cell Adhesion / Q000502:physiology; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D002852:Chromatography, Ion Exchange; D003094:Collagen / Q000378:metabolism; D003739:Dental Cementum / Q000737:chemistry* / Q000166:cytology / Q000378:metabolism; D003804:Dentin / Q000378:metabolism; D017886:Durapatite; D004591:Electrophoresis, Polyacrylamide Gel; D005109:Extracellular Matrix / Q000378:metabolism; D016326:Extracellular Matrix Proteins / Q000378:metabolism*; D005347:Fibroblasts / Q000378:metabolism; D005353:Fibronectins / Q000378:metabolism; D006882:Hydroxyapatites; D010513:Periodontal Ligament / Q000378:metabolism; D011485:Protein Binding; D011506:Proteins / Q000378:metabolism; D012038:Regeneration / Q000502:physiology; D013552:Swine; D017001:Tooth Demineralization; D014092:Tooth Root / Q000166:cytology / Q000378:metabolism*
D015815:Cell Adhesion Molecules; D016326:Extracellular Matrix Proteins; D005353:Fibronectins; D006882:Hydroxyapatites; D011506:Proteins; C433705:cementum attachment protein, 55-kDa; D003094:Collagen; D017886:Durapatite