Comparative auto-controlled study between swim-up and Percoll preparation of fresh semen samples for in-vitro fertilization.
This study compared swim-up and Percoll preparation of fresh semen samples for in-vitro fertilization. Sixty trials of in-vitro fertilization (IVF), 38 with normal semen and 22 with abnormal semen, comprising 734 oocytes were included in the study. Each semen sample was prepared by both a swim-up technique and a simplified discontinuous (50%, 70%, 90%) Percoll gradient. The oocytes for each trial were distributed at random between the two sperm preparations and incubated with the same number of motile spermatozoa. Percoll gradient preparation produced a significantly higher final concentration of spermatozoa than swim-up preparation (mean +/- SEM: 6.6 +/- 1.5 x 10(6)/ml versus 1.9 +/- 0.2 x 10(6)/ml; P less than 0.01) but a significantly lower sperm motility (69 +/- 2% versus 94 +/- 1%; P less than 0.001) and a lower number of normal forms (55 +/- 2% versus 64 +/- 2%; P less than 0.01). The ability of the Percoll gradient method to extract motile spermatozoa was higher than that of the swim-up technique (20 +/- 15.6% versus 0.8 +/- 13.6%). Nevertheless, the rates of fertilization (61%), fertilization failure (18%) and polyspermia (9%), embryo quality evaluated by mean embryo scores (3.8 +/- 0.3) and the mean number of spare embryos frozen per trial (1.4 +/- 0.3) were strictly identical in both groups. The 24 pregnancies (including three from frozen--thawed embryos) obtained in these 60 trials (40% per oocyte retrieval) could not be separated according to the sperm preparation method, as embryos from both groups were replaced together.(ABSTRACT TRUNCATED AT 250 WORDS)
Englert
Y
Y
Department of Obstetrics/Gynaecology, Erasmus Hospital-ULB, Belgium.
Van den Bergh
M
M
Rodesch
C
C
Bertrand
E
E
Biramane
J
J
Legreve
A
A
D002469:Cell Separation / Q000379:methods; D005307:Fertilization in Vitro; D006801:Humans; D008297:Male; D011205:Povidone; D012661:Semen / Q000166:cytology*; D012822:Silicon Dioxide; D013094:Spermatozoa / Q000166:cytology*
C016039:Percoll; D012822:Silicon Dioxide; D011205:Povidone