title: Crystallization and preliminary crystallographic study of an octa-heme cytochrome c3 from Desulfovibrio desulfuricans Norway.
abstract: An octa-heme cytochrome c3, isolated as a dimeric molecule of about 30 kDa from the anaerobic bacteria Desulfovibro desulfuricans Norway, has been crystallized in a form suitable for atomic resolution X-ray structural investigations. The crystals are trigonal, space group P3(1)21 (or its enantiomorph P3(2)21), with cell dimensions: a = b = 72.9 A c = 62.7 A. The asymmetric unit contains most probably one monomer and a solvent content of about 60%. Under this assumption, the crystallographic 2-fold axis relates the two subunits of the dimer. Diffraction extends to 2.0 A.
authors.affiliation: Laboratoire de Cristallographie et Cristallisation des Macromolécules Biologiques-CNRS, URA 1296, Université d'Aix-Marseille II, Faculté de Médecine Nord, France.
authors.forename: M
authors.initials: M
authors.lastname: Czjzek
authors.forename: F
authors.initials: F
authors.lastname: Guerlesquin
authors.forename: V
authors.initials: V
authors.lastname: Roig
authors.forename: F
authors.initials: F
authors.lastname: Payan
authors.forename: M
authors.initials: M
authors.lastname: Bruschi
authors.forename: R
authors.initials: R
authors.lastname: Haser
mesh_terms: D003460:Crystallization; D003574:Cytochrome c Group / Q000737:chemistry*; D003901:Desulfovibrio / Q000737:chemistry*; D011487:Protein Conformation; D014961:X-Ray Diffraction
chemical_list: D003574:Cytochrome c Group; C017165:cytochrome c(3)