Conservation of HPV-16 E6/E7 ORF sequences in a cervical carcinoma.
A cervical carcinoma that contained human papillomavirus (HPV)-16 homologous DNA was analyzed. Each tumor cell genome contained a single, incomplete copy of HPV-16 DNA. The E6 and E7 open reading frames (ORFs) were completely conserved relative to other published HPV-16 sequences. Much of the non-coding region (NCR) was free of base changes, including complete conservation of several regulatory elements. Multiple mutations were identified in the remaining integrated HPV-16 DNA, which was composed of parts of the L1 and E1 ORFs. The extraordinary conservation of the E6/E7 DNA sequence, as compared with other regions of the integrated HPV-16 DNA, supports the role of E6/E7 in tumorigenesis.
Cone
R W
RW
First Hill Women's Clinic, Seattle, Washington.
Minson
A C
AC
Smith
M R
MR
McDougall
J K
JK
D000328:Adult; D001483:Base Sequence; D003001:Cloning, Molecular; D004273:DNA, Neoplasm / Q000235:genetics* / Q000302:isolation & purification; D004279:DNA, Viral / Q000235:genetics* / Q000302:isolation & purification; D005260:Female; D005814:Genes, Viral; D006801:Humans; D008969:Molecular Sequence Data; D009857:Oncogenes; D016366:Open Reading Frames; D027383:Papillomaviridae / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D014412:Tumor Virus Infections / Q000209:etiology / Q000382:microbiology; D002583:Uterine Cervical Neoplasms / Q000209:etiology / Q000382:microbiology*
D004273:DNA, Neoplasm; D004279:DNA, Viral