[Establishment and characteristics of a lymphoblastoid cell line (CKM) resistant to 6-TG and ouabain].
Three lymphoblastoid cell lines (CKM-c, CKM-1 and CKM-8) have been established by EBV-transformation from cord blood. They have been maintained for 1 year after 120 passages with rapid growth. The population doubling times were 24.5h (CKM-1), 30 h (CKM-8) and 36 h (CKM-c). The B lymphocyte features were identified under an electron microscope. EBV antigens including EBNA, VCA-Ig A, MA and EA were negative in all the cell lines. The median chromosome number was 48-50. Interferon (IFN) was induced by NDV treatment of the CKM-c cell line. CKM-8 has been modified to be resistant to both thioguanine (30 micrograms/ml) and Ouabain (10(-5)) for the production of human-human hybridomas.
Gong
C
C
Institute of Medical Biology, Kunming.
D002461:Cell Line, Transformed / Q000187:drug effects*; D002472:Cell Transformation, Viral; D005312:Fetal Blood; D004854:Herpesvirus 4, Human / Q000502:physiology; D006801:Humans; D006825:Hybridomas; D008214:Lymphocytes / Q000166:cytology*; D010042:Ouabain / Q000494:pharmacology*; D013866:Thioguanine / Q000494:pharmacology*
D010042:Ouabain; D013866:Thioguanine