Fatty acid modulation of HepG2 cell cholesterol biosynthesis and esterification.
We investigated the effects of medium supplementation with increasing amounts of different fatty acids on HepG2 cell cholesterol biosynthesis and esterification. Up to 200 microM 16:0 led to an increase in cholesterol secretion/synthesis of 60%/40%; 18:1, 18:2, or 18:3 decreased secretion/synthesis by 35%/25%, 65%/65%, and 60%/60%; 80 microM 20:5 caused a reduction of 75%/50%. Compared to 200 microM 16:0, 18:2 led to a 50% reduced cellular cholesterol ester biosynthesis; the effect of 30 microM 20:5 was comparable to that of 18:2 while the addition of 200 microM 18:1 raised esterification. Supplementation of 18:2 reduced the cellular cholesterol ester content by 50%; 16:0 led to an increase of 80%. The effects of saturated and unsaturated fatty acids seem to be related to their number of double bonds and could be due to changes in membrane phospholipid fatty acid composition.
Schäfer
H F
HF
Institute for Clinical Chemistry, Klinikum Mannheim, University of Heidelberg, Germany.
Kattermann
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R
D006528:Carcinoma, Hepatocellular / Q000378:metabolism; D002784:Cholesterol / Q000096:biosynthesis / Q000378:metabolism*; D004951:Esterification; D005227:Fatty Acids / Q000494:pharmacology*; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism; D014407:Tumor Cells, Cultured
D005227:Fatty Acids; D002784:Cholesterol