Nerve growth factor-induced loss of cell-associated nerve growth factor receptor in human melanoma A875 cells.
Human A875 melanoma cells are known to express the low-affinity nerve growth factor receptor p75NGFR in a monomeric and a covalently linked probably dimeric form. Kinetic analysis of the association of nerve growth factor (NGF) with its receptor revealed a rapid loss of binding sites at high ligand concentrations. Using cross-linking and immunoprecipitation with an anti-p75NGFR antibody, this was found to be due to a decrease of the high molecular weight form of the receptor. Mechanisms for such a ligand-induced receptor loss are discussed.
Hertel
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C
Pharma Division, Preclinical Research, F. Hoffmann La Roche Ltd., Basel, Switzerland.
Schubenel
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R
D015536:Down-Regulation / Q000187:drug effects; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D007700:Kinetics; D008545:Melanoma / Q000473:pathology*; D009363:Neoplasm Proteins / Q000096:biosynthesis; D009414:Nerve Growth Factors / Q000494:pharmacology*; D011485:Protein Binding; D011518:Proto-Oncogene Proteins / Q000378:metabolism; D020917:Receptor, trkA; D011956:Receptors, Cell Surface / Q000378:metabolism*; D017475:Receptors, Nerve Growth Factor; D013696:Temperature; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000378:metabolism
D009363:Neoplasm Proteins; D009414:Nerve Growth Factors; D011518:Proto-Oncogene Proteins; D011956:Receptors, Cell Surface; D017475:Receptors, Nerve Growth Factor; D020917:Receptor, trkA