Purification and crystallization of intact human rhinovirus complexed with a neutralizing Fab.
We report the first crystallization of an intact virion, human rhinovirus 14, complexed with the Fab fragment from a neutralizing antibody. These crystals diffract to at least 6.0A resolution. It has been suggested that Fab's and mAb's can induce large conformational changes in the capsid upon binding. The structure of this complex should enable us to detect the existence and role of such changes.
Smith
T J
TJ
Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907.
Chase
E S
ES
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D003460:Crystallization; D006801:Humans; D007140:Immunoglobulin Fab Fragments; D008968:Molecular Conformation; D009500:Neutralization Tests; D012229:Rhinovirus / Q000302:isolation & purification* / Q000648:ultrastructure*; D014771:Virion / Q000648:ultrastructure*; D014961:X-Ray Diffraction
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D007140:Immunoglobulin Fab Fragments