cDNA cloning and structure of mouse putative Ah receptor.
Mouse cDNA clones for a putative Ah receptor have been isolated from a cDNA library of mRNA from Hepa-1 cells by an oligonucleotide probe produced by PCR with a pair of primers which was synthesized according to the reported N-terminal sequence of 26 amino acids. The cDNA clones encode a polypeptide of 805 amino acids with a helix-loop-helix motif and with some similarity to a certain region designated PAS of Drosophila Per and Sim, and human Arnt protein. Cotransfection of an expression vector of the Ah receptor with a reporter plasmid pMC6.3k consisting of CYP1A1 promoter and CAT structural gene into CV-1 cells enhanced the CAT expression in response to added 3-methylcholanthrene.
Ema
M
M
Department of Chemistry, Faculty of Science, Tohoku University, Sendai, Japan.
Sogawa
K
K
Watanabe
N
N
Chujoh
Y
Y
Matsushita
N
N
Gotoh
O
O
Funae
Y
Y
Fujii-Kuriyama
Y
Y
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics / Q000378:metabolism; D003001:Cloning, Molecular; D015723:Gene Library; D006801:Humans; D008748:Methylcholanthrene / Q000494:pharmacology; D051379:Mice; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D000072317:Polychlorinated Dibenzodioxins / Q000378:metabolism; D016133:Polymerase Chain Reaction; D018336:Receptors, Aryl Hydrocarbon; D011955:Receptors, Drug / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D014162:Transfection
D015345:Oligonucleotide Probes; D000072317:Polychlorinated Dibenzodioxins; D018336:Receptors, Aryl Hydrocarbon; D011955:Receptors, Drug; D011993:Recombinant Fusion Proteins; D008748:Methylcholanthrene; D015500:Chloramphenicol O-Acetyltransferase