A simple method to attach a universal 50-bp GC-clamp to PCR fragments used for mutation analysis by DGGE.
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Division of Experimental Therapy, The Netherlands Cancer Institute Antonin van Leeuwenhoek Huis, Amsterdam.
D001482:Base Composition; D001483:Base Sequence; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics; D003596:Cytosine; D004252:DNA Mutational Analysis / Q000379:methods*; D004273:DNA, Neoplasm / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D016158:Genes, p53; D006151:Guanosine; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics; D008969:Molecular Sequence Data; D009691:Nucleic Acid Denaturation; D009838:Oligodeoxyribonucleotides; D016133:Polymerase Chain Reaction
D004273:DNA, Neoplasm; D009838:Oligodeoxyribonucleotides; D006151:Guanosine; D003596:Cytosine