title: Crystallization and preliminary X-ray analysis of three serotypes of foot-and-mouth disease virus.
abstract: Foot-and-mouth disease viruses from serotypes O, A and C have been crystallized. The particular strains studied include O1K, A10(61), A22 Iraq 24/64, A24 Cruzeiro and C-S8c1. In addition, crystals have been grown of G67, a monoclonal antibody neutralization escape mutant derived from O1K, and of virus R100, recovered after the establishment of a persistent infection in baby hamster kidney cells with C-S8c1. Empty particles, capsids which lack the RNA genome, have also been crystallized for subtypes A22 Iraq 24/64 and A10(61). In almost all cases, crystals suitable for high resolution structure determination were obtained from (NH4)2SO4 or mixtures of polyethylene glycol and NH4Cl.
authors.affiliation: AFRC Institute for Animal Health, Pirbright Laboratory, Woking, U.K.
authors.forename: S
authors.initials: S
authors.lastname: Curry
authors.forename: R
authors.initials: R
authors.lastname: Abu-Ghazaleh
authors.forename: W
authors.initials: W
authors.lastname: Blakemore
authors.forename: E
authors.initials: E
authors.lastname: Fry
authors.forename: T
authors.initials: T
authors.lastname: Jackson
authors.forename: A
authors.initials: A
authors.lastname: King
authors.forename: S
authors.initials: S
authors.lastname: Lea
authors.forename: D
authors.initials: D
authors.lastname: Logan
authors.forename: J
authors.initials: J
authors.lastname: Newman
authors.forename: D
authors.initials: D
authors.lastname: Stuart
mesh_terms: D005537:Aphthovirus / Q000737:chemistry* / Q000235:genetics / Q000648:ultrastructure; D002213:Capsid / Q000737:chemistry / Q000648:ultrastructure; D003460:Crystallization; D014644:Genetic Variation; D009154:Mutation; D012703:Serotyping; D014357:Trypsin / Q000494:pharmacology; D014771:Virion / Q000737:chemistry / Q000187:drug effects / Q000235:genetics / Q000648:ultrastructure; D014961:X-Ray Diffraction
chemical_list: D014357:Trypsin