[Cytochrome oxidase induction following oxidative stress produced by adriamycin in the heart of rats fed olive oil].
Cytochrome oxidase is extremely susceptible to modifications that could occur at membrane level such as, for example, the deep influence that cardiolipin has on its maximal activity. Moreover, even the different ratio of fatty acid species in cardiolipin molecule could affect cytochrome oxidase properties. The influence of dietary fat and of endogenous lipid peroxidation with regard to cytochrome c oxidase activity and cytochrome a+a3 concentrations in mitochondria from rat heart has been investigated. Whilst the endogenous oxidative stress led to increase the peroxidation products, detected by means of hydroperoxides and conjugated dienes determinations, the features of the lipid environment were not affected as shown by fluorescence polarization technique. However, the oxidative stress was able to induce a significant increase of cytochrome a+a3 level even if the phenomenon did not reach the same extent as in the case of previous investigations on rat liver. Moreover, the results vary with the different fat source used and this might indicate a possible involvement of different dietary fats in peroxidation mechanisms. Furthermore the data we have obtained enable us to confirm once more that an induction of the synthesis of cytochrome a+a3 might be related to an enhanced production of peroxides at membrane level.
Huertas
J R
JR
Departamento di Fisiologia, Universidad de Granada, Spagna.
Battino
M
M
Mataix
F
F
Littarru
G P
GP
Lenaz
G
G
D000818:Animals; D003314:Corn Oil / Q000008:administration & dosage; D004042:Dietary Fats, Unsaturated / Q000008:administration & dosage*; D004317:Doxorubicin / Q000494:pharmacology*; D003576:Electron Transport Complex IV / Q000096:biosynthesis* / Q000187:drug effects; D004790:Enzyme Induction / Q000187:drug effects; D006321:Heart / Q000187:drug effects*; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D009206:Myocardium / Q000201:enzymology*; D000069463:Olive Oil; D010938:Plant Oils / Q000008:administration & dosage*; D051381:Rats; D017208:Rats, Wistar; D013312:Stress, Physiological / Q000178:diet therapy / Q000201:enzymology*
D004042:Dietary Fats, Unsaturated; D000069463:Olive Oil; D010938:Plant Oils; D003314:Corn Oil; D004317:Doxorubicin; D003576:Electron Transport Complex IV
Induzione della citocromo ossidasi in seguito a stress ossidativo prodotto da adriamicina in cuore di ratti alimentati con olio di oliva.