title: Peptides presented to the immune system by the murine class II major histocompatibility complex molecule I-Ad.
abstract: Between 650 and 2000 different peptides are associated with the major histocompatibility complex class II molecule I-Ad. Sequences for nine of these were obtained by a combination of automated Edman degradation and tandem mass spectrometry. All of the peptides are derived from secretory or integral membrane proteins that are synthesized by the antigen-presenting cell itself. Peptides were 16 to 18 residues long, had ragged NH2-and COOH-termini, and contained a six-residue binding motif that was variably placed within the peptide chain. Binding data on truncated peptides suggest that the peptide binding groove on class II molecules can be open at both ends.
authors.affiliation: Department of Chemistry, University of Virginia, Charlottesville 22901.
authors.forename: D F
authors.initials: DF
authors.lastname: Hunt
authors.forename: H
authors.initials: H
authors.lastname: Michel
authors.forename: T A
authors.initials: TA
authors.lastname: Dickinson
authors.forename: J
authors.initials: J
authors.lastname: Shabanowitz
authors.forename: A L
authors.initials: AL
authors.lastname: Cox
authors.forename: K
authors.initials: K
authors.lastname: Sakaguchi
authors.forename: E
authors.initials: E
authors.lastname: Appella
authors.forename: H M
authors.initials: HM
authors.lastname: Grey
authors.forename: A
authors.initials: A
authors.lastname: Sette
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000938:Antigen-Presenting Cells / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D000949:Histocompatibility Antigens Class II / Q000276:immunology*; D013058:Mass Spectrometry; D008565:Membrane Proteins / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D012689:Sequence Homology, Nucleic Acid
chemical_list: D000949:Histocompatibility Antigens Class II; D008565:Membrane Proteins