title: The N-linked oligosaccharides of human lactoferrin are not required for binding to bacterial lactoferrin receptors.
abstract: The oligosaccharides of human lactoferrin were enzymatically removed with glycopeptidase F, resulting in a preparation containing partial and fully deglycosylated human lactoferrin. The derivatives were separated by Concanavalin A affinity chromatography and compared with native human lactoferrin with respect to their ability to bind to bacterial receptors. Competitive binding experiments demonstrated that the lactoferrin derivatives were equally capable as native lactoferrin in binding to receptors of Neisseria meningitidis, Neisseria gonorrhoeae, and Moraxella catarrhalis. This result indicates that the oligosaccharides on human lactoferrin are not essential for binding to the bacterial receptors.
authors.affiliation: Department of Microbiology and Infectious Diseases, University of Calgary, Alta., Canada.
authors.forename: J
authors.initials: J
authors.lastname: Alcantara
authors.forename: J S
authors.initials: JS
authors.lastname: Padda
authors.forename: A B
authors.initials: AB
authors.lastname: Schryvers
mesh_terms: D001665:Binding Sites; D006801:Humans; D066298:In Vitro Techniques; D007781:Lactoferrin / Q000737:chemistry / Q000378:metabolism*; D001936:Moraxella catarrhalis / Q000378:metabolism; D009344:Neisseria gonorrhoeae / Q000378:metabolism; D009345:Neisseria meningitidis / Q000378:metabolism; D009346:Neisseriaceae / Q000378:metabolism*; D009844:Oligosaccharides / Q000737:chemistry / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000378:metabolism*
chemical_list: D009844:Oligosaccharides; D011956:Receptors, Cell Surface; C038340:lactoferrin receptors; D007781:Lactoferrin