Induction of a BrdU-enhanceable fragile site-like lesion and sister chromatid exchanges at 11q23.1 in EBV-transformed lymphoblastoid cell lines.
We examined the expression of a fragile site-like lesion and induction of sister chromatid exchanges (SCEs) at 11q23.1 in EBV-transformed lymphoblastoid cell lines derived from carriers of distamycin A-inducible fragile sites and ataxia telangiectasia patients. The fragile site-like lesion at 11q23.1 was found to be BrdU-enhanceable in all cell lines examined, and the expression frequencies increased linearly with the rates of BrdU substitution in replicated DNA. In addition, an increased frequency of SCEs was observed at 11q23.1 on the expressed chromosome. Thus, the BrdU-enhanceable fragile site-like lesion at 11q23.1 is a "hot spot" for the formation of SCEs, as has been reported for other rare and common fragile sites.
Seki
N
N
Division of Genetics, National Institute of Radiological Sciences, Chiba, Japan.
Tsuji
H
H
Takahashi
E
E
Yamauchi
M
M
Saito
T
T
Hashimoto
T
T
Yamamoto
K
K
Hori
T
T
D001973:Bromodeoxyuridine / Q000494:pharmacology*; D002460:Cell Line; D002461:Cell Line, Transformed; D002871:Chromosome Banding; D043283:Chromosome Fragile Sites; D002873:Chromosome Fragility; D002880:Chromosomes, Human, Pair 11; D004305:Dose-Response Relationship, Drug; D004854:Herpesvirus 4, Human / Q000235:genetics*; D006801:Humans; D007621:Karyotyping; D007700:Kinetics; D008214:Lymphocytes; D012854:Sister Chromatid Exchange / Q000187:drug effects
D001973:Bromodeoxyuridine