title: Lipid peroxidation in human breast cancer cells in response to gamma-linolenic acid and iron.
abstract: Lipid peroxidation in human breast cancer (ZR-75-1) cells and cancer cell killing were confirmed by using ultraviolet (UV)-spectrophotometry and mass spectrometry (MS). ZR-75-1 cells and human normal fibroblast CCD-41-SK (41Sk) cells were cultured with gamma-linolenic acid (GLA) and ferrous iron Fe (II) combinations. Formation of lipid peroxide and cytotoxic effect were highest in ZR-75-1 cells treated with GLA + Fe (II), though 41Sk cells showed little evidence of either lipid peroxidation or cytotoxity. These results indicate a cancer-cell-specific lipid peroxidation mechanism in association with the selective cancer cell killing effect in response to GLA.
authors.affiliation: Efamnol Research Institute, Kentville, Nova Scotia, Canada.
authors.forename: S
authors.initials: S
authors.lastname: Takeda
authors.forename: D F
authors.initials: DF
authors.lastname: Horrobin
authors.forename: M
authors.initials: M
authors.lastname: Manku
authors.forename: P G
authors.initials: PG
authors.lastname: Sim
authors.forename: G
authors.initials: G
authors.lastname: Ells
authors.forename: V
authors.initials: V
authors.lastname: Simmons
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D001943:Breast Neoplasms / Q000378:metabolism* / Q000473:pathology; D002470:Cell Survival / Q000187:drug effects; D005260:Female; D006801:Humans; D007501:Iron / Q000494:pharmacology*; D008042:Linolenic Acids / Q000494:pharmacology*; D015227:Lipid Peroxidation / Q000187:drug effects*; D014407:Tumor Cells, Cultured; D017965:gamma-Linolenic Acid
chemical_list: D000970:Antineoplastic Agents; D008042:Linolenic Acids; D017965:gamma-Linolenic Acid; D007501:Iron