[Evaluation of the antithrombotic effects of pentoxifylline, acetylsalicylic acid and low molecular weight heparin in the laser model of thrombosis].
Antithrombotic effects of different agents including acetylsalicylic acid (Asprocol/Polfa PL/), pentoxifylline (Agapurin/Spofa CS/) and low molecular weight heparin (Antixarin B. Braun, Melsungen, FRG) were studied in the laser-induced rat thrombosis model. The investigations were carried out on male Wistar rats weighing 200-300 g. Thrombus formation was induced in small mesenteric arteries 25-30 microns; using argon laser. An interference contrast system based on a Leitz Orthoplan microscope for the evaluation of thrombus formation was used. The number of laser injuries needed to induce a defined thrombus proved to be a useful way to quantitate the results in this thrombosis mode. All agents showed dose dependent antithrombotic effect in our laser model. Acetylsalicylic acid (ASA) 2 hours after oral administration markedly inhibited thrombus formation in minimal dose 50 mg/kg, pentoxifylline in dose 10 mg/kg 30 min after i.v. injection and low molecular weight heparin (Antixarin) in dose 0.1 mg/kg, 2 hours, after s.c. injection. Antithrombotic effect of administration of minimal effective doses of ASA (orally) and pentoxifylline (i.v.) lasted longer than 4 hours but less then 6 hours. LMW heparin (Antixarin) in minimal effective dose 0.1 mg/kg after s.c. injection inhibited thrombus formation in small mesenteric vessels for more than 12 hours but less than 24 hours.
Krupiński
K
K
Klinika Hematologii Akademii Medycznej Białymstoku.
Breddin
H K
HK
Bielawiec
M
M
Płonowski
A
A
D000818:Animals; D001241:Aspirin / Q000494:pharmacology*; D004195:Disease Models, Animal; D006495:Heparin, Low-Molecular-Weight / Q000494:pharmacology*; D066298:In Vitro Techniques; D007834:Lasers; D008297:Male; D010431:Pentoxifylline / Q000494:pharmacology*; D013152:Splanchnic Circulation / Q000187:drug effects; D015912:Thrombolytic Therapy; D013927:Thrombosis / Q000188:drug therapy*
D006495:Heparin, Low-Molecular-Weight; D001241:Aspirin; D010431:Pentoxifylline
Ocena przeciwzakrzepowych właściwości pentoksyfiliny,kwasu acetylsalicylowego oraz drobnoczasteczkowej heparyny na laserowym modelu zakrzepowym.