title: Phosphodiesters in saponified extracts of human breast and colon tumors using 31P magnetic resonance spectroscopy.
abstract: Saponified phospholipid extracts of malignant and normal human breast and colon surgical tissue specimens (n = 45) generate characteristic phosphodiester profiles using 31P magnetic resonance (MR) spectroscopy. The resultant 31P MR spectroscopic profiles of the analyzed tissues are used to differentiate malignant from normal. The appearance of an uncharacterized resonance at 0.29 delta in the malignant tissue spectra (50% of breast and 75% of colon specimens) is the most notable qualitative finding. Quantitatively, malignant colon tissues differ from normal colon tissues with depressed levels of phosphatidylserine and elevated levels of glycerol 3-phosphorylglycerol and an index measuring the summation of phospholipid polar head group residues with free hydroxyl groups. Malignant breast tissues have significantly elevated levels of glycerol 3-phosphorylethanolamine and significantly depressed levels of glycerol 3-phosphorylcholine compared to normal breast tissues, reflecting a perturbation in the balance of lipid residues that are the respective breakdown products of phosphatidylethanolamine and phosphatidylcholine. The concentration of the polar head group residues is compared to 31P MR spectroscopic profiles of colon and breast tissue phospholipids, in order to demonstrate the quantitative nature of the technique employed.
authors.affiliation: Pathology Institute, University of Utrecht, The Netherlands.
authors.forename: T E
authors.initials: TE
authors.lastname: Merchant
authors.forename: D
authors.initials: D
authors.lastname: Characiejus
authors.forename: J N
authors.initials: JN
authors.lastname: Kasimos
authors.forename: W
authors.initials: W
authors.lastname: Den Otter
authors.forename: L W
authors.initials: LW
authors.lastname: Gierke
authors.forename: T
authors.initials: T
authors.lastname: Glonek
mesh_terms: D000230:Adenocarcinoma / Q000737:chemistry*; D001943:Breast Neoplasms / Q000737:chemistry*; D002285:Carcinoma, Intraductal, Noninfiltrating / Q000737:chemistry*; D003110:Colonic Neoplasms / Q000737:chemistry*; D005260:Female; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D010743:Phospholipids / Q000378:metabolism*
chemical_list: D010743:Phospholipids