title: Cytomegalovirus genomes demonstrated by polymerase chain reaction in synovial fluid from rheumatoid arthritis patients.
abstract: Synovial fluid from 20 patients with rheumatoid arthritis was analyzed to detect human cytomegalovirus (CMV) genomic material using polymerase chain reaction. Of 20 samples tested, 9 were positive for CMV by either ethidium bromide staining or Southern blotting. In contrast, CMV was detected in only 2 of 18 control patients with osteoarthritis. These results suggest an etiologic relationship between CMV and rheumatoid arthritis.
authors.affiliation: Department of Microbiology, Kanazawa Medical University, Ishikawa, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Murayama
authors.forename: F
authors.initials: F
authors.lastname: Jisaki
authors.forename: M
authors.initials: M
authors.lastname: Ayata
authors.forename: D
authors.initials: D
authors.lastname: Sakamuro
authors.forename: T
authors.initials: T
authors.lastname: Hironaka
authors.forename: K
authors.initials: K
authors.lastname: Hirai
authors.forename: N
authors.initials: N
authors.lastname: Tsuchiya
authors.forename: K
authors.initials: K
authors.lastname: Ito
authors.forename: T
authors.initials: T
authors.lastname: Furukawa
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001172:Arthritis, Rheumatoid / Q000150:complications / Q000235:genetics* / Q000382:microbiology; D001483:Base Sequence; D015139:Blotting, Southern; D003587:Cytomegalovirus / Q000235:genetics* / Q000302:isolation & purification; D003586:Cytomegalovirus Infections / Q000150:complications; D004279:DNA, Viral / Q000032:analysis* / Q000235:genetics*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D010003:Osteoarthritis / Q000150:complications / Q000235:genetics / Q000382:microbiology; D016133:Polymerase Chain Reaction; D013582:Synovial Fluid / Q000737:chemistry* / Q000382:microbiology
chemical_list: D004279:DNA, Viral