Characterization of monoclonal antibodies to the outer membrane protein (OmpD) of Salmonella typhimurium.
A panel of monoclonal antibodies, seven against the trimeric and seven against the monomeric forms to outer membrane protein D (OmpD) of Salmonella typhimurium were produced. The specificities of these monoclonal antibodies for the porin proteins of S. typhimurium and their cross-reactions with Salmonella porins OmpC and OmpF were determined by Western immunoblotting and enzyme-linked immunosorbent assay. We observed that OmpD shared more epitopes and had greater structural similarity with OmpC than with OmpF.
Pai
S R
SR
Biomedical Research Program, Alabama State University, Montgomery 36101.
Upshaw
Y
Y
Singh
S P
SP
D000818:Animals; D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry / Q000276:immunology*; D003429:Cross Reactions; D051379:Mice; D018272:Porins; D011487:Protein Conformation; D012486:Salmonella typhimurium / Q000276:immunology*
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D018272:Porins