title: Japanese soybean paste miso scavenges free radicals and inhibits lipid peroxidation.
abstract: Japanese soybean paste miso, which has been reported to prevent gastric and mammary cancer and chronic nephritis, was demonstrated by electron spin resonance spectrometry using 5,5'-dimethyl-1-pyrroline-N-oxide as a scavenger of free radicals. Fifty mg/ml of miso scavenged 100% of 1,1-diphenyl-2-picrylhydrazyl radicals (3.9 x 10(15) spins/ml); 45 mg/ml quenched 92% of hydroxyl radicals (7.9 x 10(16) spins/ml); and 50 mg/ml quenched 50% of superoxide anion (6.7 x 10(16) spins/ml). In the system of rat cerebral cortex homogenate supplemented with 2 mM each of Fe2+ and ascorbic acid, 90% and 82% of the hydrogen and carbon-centered radicals having 1.7 x 10(13) spins/ml and 3.9 x 10(13) spins/ml, respectively, were quenched by 180 mg/ml of miso. The thiobarbituric acid-reactive substances, an index of lipid peroxidation in the brain, was inhibited by 10 mg/ml of miso. These results showed that miso acts as an antioxidant by scavenging free radicals.
authors.affiliation: Department of Neurochemistry, Okayama University Medical School, Japan.
authors.forename: L A
authors.initials: LA
authors.lastname: Santiago
authors.forename: M
authors.initials: M
authors.lastname: Hiramatsu
authors.forename: A
authors.initials: A
authors.lastname: Mori
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D001205:Ascorbic Acid / Q000494:pharmacology; D002540:Cerebral Cortex / Q000737:chemistry / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004578:Electron Spin Resonance Spectroscopy; D016166:Free Radical Scavengers; D015227:Lipid Peroxidation / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013025:Glycine max; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis; D014810:Vitamin E / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D016166:Free Radical Scavengers; D017392:Thiobarbituric Acid Reactive Substances; D014810:Vitamin E; D001205:Ascorbic Acid