title: Effects of equine infectious anemia virus on hematopoietic progenitors in vitro.
abstract: Direct effects of equine infectious anemia virus (EIAV) on hematopoiesis in vitro were studied. Bone marrow mononuclear cells from clinically normal horses were incubated with 100 TCID50 of EIAV/10(7) cells. These cells were cultured to assay for colonies derived from erythroid progenitors, granulocyte/monocyte progenitors, and fibroblastic progenitors. The EIAV had a selective suppressive effect on the erythroid progenitors. Colony-forming units-erythroid were suppressed to 80% of that for medium controls (P = 0.011). Burst-forming units-erythroid were suppressed to 70% of that for medium controls (P = 0.003). Significant effect was not apparent on colony-forming units-granulocyte/macrophage or on colony-forming units-fibroblastic.
authors.affiliation: Department of Veterinary Pathobiology, College of Veterinary Medicine, Ohio State University, Columbus 43210.
authors.forename: C J
authors.initials: CJ
authors.lastname: Swardson
authors.forename: G J
authors.initials: GJ
authors.lastname: Kociba
authors.forename: L E
authors.initials: LE
authors.lastname: Perryman
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells; D002478:Cells, Cultured; D005347:Fibroblasts / Q000382:microbiology; D006098:Granulocytes / Q000382:microbiology; D006412:Hematopoietic Stem Cells / Q000382:microbiology*; D004860:Infectious Anemia Virus, Equine / Q000502:physiology*; D008264:Macrophages / Q000382:microbiology