A rabbit corneal epithelial cell line expresses functional platelet-derived growth factor beta-type receptors.
Platelet-derived growth factor (PDGF) is a family of three isoforms termed PDGF-AA, PDGF-AB, or PDGF-BB that induce proliferation in various mesenchymal cells. A rabbit corneal epithelial cell line (SIRC) was chosen to study the effects of the three isoforms of PDGF. These cells express approximately 43,000 PDGF beta-type receptors and less than 2800 alpha-type receptors on their surface. Thus, only PDGF-BB and -AB led to a transient dose-dependent rise in cytosolic free calcium with an effective dose for 50% of cells of 5 ng/ml. The PDGF-induced calcium signal is largely independent of the presence of extracellular calcium.
Knorr
M
M
Department of General Ophthalmology, University of Tübingen, Germany.
Steuhl
K P
KP
Tatje
D
D
Hoppe
J
J
D000818:Animals; D002118:Calcium / Q000378:metabolism; D002460:Cell Line; D003315:Cornea / Q000166:cytology / Q000378:metabolism*; D004847:Epithelial Cells; D004848:Epithelium / Q000378:metabolism; D008970:Molecular Weight; D010982:Platelet-Derived Growth Factor / Q000378:metabolism*; D011817:Rabbits; D011956:Receptors, Cell Surface / Q000378:metabolism*; D017479:Receptors, Platelet-Derived Growth Factor; D011994:Recombinant Proteins / Q000378:metabolism
D010982:Platelet-Derived Growth Factor; D011956:Receptors, Cell Surface; D011994:Recombinant Proteins; D017479:Receptors, Platelet-Derived Growth Factor; D002118:Calcium