title: Chronic ethanol intake inhibits both the vasoactive intestinal peptide binding and the associated cyclic AMP production in rat enterocytes.
abstract: 1. Chronic ethanol intake during 6, 8, 10 or 12 weeks resulted in a decrease of 125I-vasoactive intestinal peptide (VIP) binding to rat enterocytes. 2. Native peptide displaced 125I-VIP binding to enterocytes, exhibiting a IC50 at about 4 nM native VIP in control and ethanol-treated animals. 3. The number of binding sites in ethanol-treated animals were significantly diminished when compared to control animals. This reduction is observed in both the high-affinity and the low-affinity binding sites. 4. Increasing concentrations of native VIP produced a similar cyclic AMP rise in enterocytes from control or ethanol-treated rats during 6 weeks. However, after 8 weeks of ethanol treatment, a significant decrease in cyclic AMP production stimulated by VIP was observed.
authors.affiliation: Department of Medical Biochemistry and Molecular Biology, University of Seville School of Medicine, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Jimenez
authors.forename: J R
authors.initials: JR
authors.lastname: Calvo
authors.forename: P
authors.initials: P
authors.lastname: Molinero
authors.forename: R
authors.initials: R
authors.lastname: Goberna
authors.forename: J M
authors.initials: JM
authors.lastname: Guerrero
mesh_terms: D000818:Animals; D000242:Cyclic AMP / Q000096:biosynthesis*; D004247:DNA / Q000378:metabolism; D004847:Epithelial Cells; D004848:Epithelium / Q000187:drug effects / Q000378:metabolism; D000431:Ethanol / Q000097:blood / Q000494:pharmacology*; D007413:Intestinal Mucosa / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D007457:Iodine Radioisotopes; D008297:Male; D011506:Proteins / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D014660:Vasoactive Intestinal Peptide / Q000378:metabolism*
chemical_list: D007457:Iodine Radioisotopes; D011506:Proteins; D014660:Vasoactive Intestinal Peptide; D000431:Ethanol; D004247:DNA; D000242:Cyclic AMP