Cigarette smoke exposure increases the cell water organization and membrane order of cultured T cells.
In order to determine the effects of cigarette smoke (CS) exposure on the physical properties of cells, NMR water-proton relaxation time (which measures the intracellular water organization) and ESR spin labeling (which measures membrane order) measurements were performed on cultured Jurkat T cells exposed to CS. NMR spin-lattice relaxation time (T1) decreased with CS exposure in a dose-dependent fashion. A significantly depressed T1 value was obtained even when CS was delivered through a filter. Cell viability was not affected in this condition. Superoxide dismutase (SOD) prevented the depression of T1 value. These results suggest that superoxide radicals or subsequently generated species contained in the gas phase of CS increase the intracellular water organization in viable cells. CS exposure also increased the ESR membrane order parameter of nitroxide spin label. These physical characteristic changes may be important in CS-induced cell responses and cytopathology.
Suzuki
Y J
YJ
Department of Molecular & Cell Biology, University of California, Berkeley 94720.
Tsuchiya
M
M
Shindo
Y
Y
Packer
L
L
D002462:Cell Membrane / Q000648:ultrastructure*; D002470:Cell Survival; D004578:Electron Spin Resonance Spectroscopy; D006801:Humans; D066298:In Vitro Techniques; D016399:Lymphoma, T-Cell; D009682:Magnetic Resonance Spectroscopy; D008560:Membrane Fluidity; D010947:Plants, Toxic; D012906:Smoke; D014026:Nicotiana; D014407:Tumor Cells, Cultured; D014867:Water / Q000737:chemistry*
D012906:Smoke; D014867:Water