Immunocytochemical studies on light-induced changes in phosphatidylinositol 4,5-bisphosphate immunoreactivity in the visual system of normal and norpA mutant of Drosophila.
The distribution of phosphatidylinositol 4,5-bisphosphate (PIP2) in the visual system of Drosophila was studied by indirect immunofluorescence staining using a monoclonal antibody against PIP2. The retina of the compound eye and the cortical regions of the optic lobe were heavily stained by the antibody. In the retina, photoreceptor cells were stained with the antibody, but non-neuronal cells such as pigment cells and cone cells were not stained. The staining intensity in the light-adapted photoreceptor cells was lower than that in dark-adapted cells in normal flies, whereas no such difference in immunoreactivity was observed in the norpAEE5 mutant, whose photoreceptor cells are deficient in phospholipase C. These results suggest that the PIP2 in the photoreceptor cells is hydrolyzed by phospholipase C coded by the norpA gene upon light stimulation.
Suzuki
E
E
Department of Fine Morphology, University of Tokyo, Japan.
Hirosawa
K
K
D000818:Animals; D000911:Antibodies, Monoclonal; D004331:Drosophila melanogaster / Q000235:genetics / Q000378:metabolism*; D007150:Immunohistochemistry; D008027:Light; D009154:Mutation; D019269:Phosphatidylinositol 4,5-Diphosphate; D010716:Phosphatidylinositols / Q000032:analysis / Q000378:metabolism*; D010786:Photoreceptor Cells / Q000378:metabolism; D012160:Retina / Q000166:cytology / Q000378:metabolism
D000911:Antibodies, Monoclonal; D019269:Phosphatidylinositol 4,5-Diphosphate; D010716:Phosphatidylinositols