Detection of functional interferon alpha receptors in human neuroendocrine tumor cell lines using a new monoclonal antibody.
While first described as antiviral agents, interferons (IFNs) exhibit significant antiproliferative and antitumor effects as well. IFN alpha has been successfully used in clinical trials to treat several malignancies, including leukemias and certain solid tumors. While many cell types have been studied for IFN alpha receptor expression, very little is known about receptor expression on human neuroendocrine cells. Using a novel anti-IFN alpha receptor monoclonal antibody, we examined IFN alpha receptor expression in 10 human cell lines derived from tumors of neuroendocrine origin, including neuroblastoma, neuroepithelioma and small cell lung carcinoma. All cell lines studied displayed a similar pattern of IFN alpha receptor expression and 5 of 8 cell lines demonstrated reduced thymidine incorporation following IFN alpha treatment. Addition of exogenous IFN alpha caused a decrease in IFN alpha receptor expression, while differentiating agents, such as phorbol esters and retinoic acid, induced an increase in receptor number without altering receptor affinity.
Rosolen
A
A
Tumor Cell Biology Section, NCI, NIH, Bethesda, MD.
Colamonici
O R
OR
Pfeffer
L M
LM
Whitesell
L
L
Nordan
R
R
Neckers
L M
LM
D000911:Antibodies, Monoclonal; D018288:Carcinoma, Small Cell / Q000378:metabolism*; D003432:Cross-Linking Reagents; D004273:DNA, Neoplasm / Q000096:biosynthesis; D005434:Flow Cytometry; D006801:Humans; D016898:Interferon-alpha / Q000378:metabolism*; D009447:Neuroblastoma / Q000378:metabolism*; D018241:Neuroectodermal Tumors, Primitive, Peripheral / Q000378:metabolism*; D011233:Precipitin Tests; D011971:Receptors, Immunologic / Q000378:metabolism*; D017471:Receptors, Interferon; D013379:Substrate Specificity; D013936:Thymidine / Q000378:metabolism; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D003432:Cross-Linking Reagents; D004273:DNA, Neoplasm; D016898:Interferon-alpha; D011971:Receptors, Immunologic; D017471:Receptors, Interferon; D013936:Thymidine