Membrane-potential-dependent uptake of tryptamine by rat intestinal brush-border membrane vesicles.
The effect of membrane potential on the uptake of tryptamine, an organic cation, by rat intestinal brush-border membrane vesicles was studied. In the presence of an outwardly directed H(+)-gradient, the initial uptake of tryptamine was stimulated remarkably and the overshoot phenomenon was observed. In contrast, the uptake was depressed by an inwardly-directed H(+)-gradient. The effect of H(+)-gradient on the uptake of tryptamine was maintained in the presence of FCCP, whereas it vanished when voltage-clamped vesicles were used. Moreover, the uptake of tryptamine was linearly augmented with increase of the valinomycin-induced inside-negative K+ diffusion potential. These results suggest that tryptamine is taken up into intestinal brush-border membrane vesicles depends upon the ionic diffusion potential. The effect of several indole derivatives and amine compounds on the uptake of tryptamine was also examined. The uptake of tryptamine was inhibited by all amine compounds used, but anionic and zwitterionic compounds had no effect, suggesting that these amines interact on brush-border membrane and cause an inhibitory effect.
Sugawara
M
M
Department of Pharmacy, Hokkaido University Hospital, School of Medicine, Sapporo, Japan.
Sasaki
M
M
Iseki
K
K
Miyazaki
K
K
D000588:Amines / Q000494:pharmacology; D000818:Animals; D002259:Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone / Q000494:pharmacology; D004058:Diffusion; D007413:Intestinal Mucosa / Q000378:metabolism*; D007422:Intestines / Q000648:ultrastructure; D007700:Kinetics; D008564:Membrane Potentials; D008871:Microvilli / Q000378:metabolism; D011188:Potassium / Q000378:metabolism; D011522:Protons; D051381:Rats; D012964:Sodium / Q000378:metabolism; D014363:Tryptamines / Q000378:metabolism*
D000588:Amines; D011522:Protons; D014363:Tryptamines; D002259:Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone; C030820:tryptamine; D012964:Sodium; D011188:Potassium