Expression of the Marek's disease virus (MDV) homolog of glycoprotein B of herpes simplex virus by a recombinant baculovirus and its identification as the B antigen (gp100, gp60, gp49) of MDV.
A gene encoding a homolog of glycoprotein B of herpes simplex virus (gB homolog) has been identified on the Marek's disease virus (MDV) genome (L. J. N. Ross, M. Sanderson, S. D. Scott, M. M. Binns, T. Doel, and B. Milne, J. Gen. Virol. 70:1789-1804, 1989); however, the molecular and immunological characteristics of the gene product(s) are still not clear. In the present study, the gB homolog of MDV was expressed in insect cells by a recombinant baculovirus, and it was characterized to determine its molecular and antigenic properties. The expressed recombinant protein had three molecular sizes (88 to 110, 58, and 49 kDa) and was recognized by antisera from chickens inoculated with each of the three serotypes of MDV. By immunofluorescence analysis, it was shown that the protein was expressed in the cytoplasm and on the surface of the recombinant baculovirus-infected cells. The gB homolog of MDV was processed similarly to pseudorabies virus and varicella-zoster virus with respect to cleavage and the intramolecular disulfide bond between the cleaved products. Interestingly, the expressed protein reacted with monoclonal antibody M51, specific to the B antigen (gp100, gp60, gp49) of MDV, although the locations of the gene encoding the B antigen and of the gene encoding the gB homolog were reported to be different. Moreover, competitive experiments revealed that anti-gB homolog serum and monoclonal antibody M51 recognized the same molecules. From these results, the gB homolog and the B antigen of MDV seem to be the same glycoprotein.
Niikura
M
M
Department of Veterinary Microbiology, Faculty of Agriculture, University of Tokyo, Japan.
Matsuura
Y
Y
Endoh
D
D
Onuma
M
M
Mikami
T
T
D000818:Animals; D000956:Antigens, Viral / Q000096:biosynthesis* / Q000276:immunology; D016367:Baculoviridae / Q000235:genetics; D001483:Base Sequence; D002478:Cells, Cultured; D003429:Cross Reactions; D005822:Genetic Vectors; D008381:Herpesvirus 2, Gallid / Q000235:genetics* / Q000276:immunology; D007150:Immunohistochemistry; D007313:Insecta; D008969:Molecular Sequence Data; D011499:Protein Processing, Post-Translational; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000276:immunology; D012703:Serotyping; D018139:Simplexvirus / Q000276:immunology; D014162:Transfection; D014759:Viral Envelope Proteins / Q000096:biosynthesis* / Q000276:immunology
D000956:Antigens, Viral; D011994:Recombinant Proteins; D014759:Viral Envelope Proteins