Estimation of the infectivity of herpes simplex virus type I passage III on two different tissue culture.
This technique has been developed for the estimation of the infectivity of herpes simplex virus type I passage III on a tissue culture of Vero and HBK21. The plaque assay method was performed and the number of plaques on the two culture media was counted per each dilution from (10(-5) to 10(-8)). It was found that the number and the size of plaques in 10(-5) dilution are the best in the two types of tissue cultures. The number indicates the number of virus particle (per each dilution point), which can be used for infection of experimental animals to demonstrate its infectivity character.
Arafa
R M
RM
Department of Microbiology, Benha Faculty of Medicine.
D000818:Animals; D015169:Colony Count, Microbial; D046508:Culture Techniques; D018139:Simplexvirus / Q000254:growth & development*; D010948:Viral Plaque Assay / Q000379:methods*; D014776:Virus Cultivation