title: Effect of rat atrial natriuretic factor on in vivo and in vitro aldosterone and corticosterone secretions in the rat during the perinatal period.
abstract: The effect of rat atrial natriuretic factor (rANF) on aldosterone and corticosterone secretion was investigated in vivo in 21-day-old rat fetuses injected intravenously through the umbilical vein and in vitro on isolated adrenal cells from 17-, 19- and 21-day-old fetuses and 1-, 2-, 3- and 4-week-old rats. In vivo, rANF (50 pmol/50 microliters/fetus) inhibited both basal levels and secretion of aldosterone stimulated by adrenocorticotropin hormone (ACTH(1-24), 0.25 pmol/50 microliters/fetus), but not corticosterone secretion. In vitro, the addition of graded concentrations of rANF (0.001, 0.01 and 10 nmol/l) to the incubation medium did not affect the basal aldosterone and corticosterone secretions of fetal and neonatal adrenal cells. ACTH(1-24) (0.1 nmol/l) stimulated productions of both corticosterone and aldosterone by the adrenal cells at all stages studied. The addition of graded concentrations of rANF to the incubation medium containing ACTH(1-24) (0.1 nmol/l) induced a dose-dependent inhibition of aldosterone secretion by the adrenal cells from 21-day-old fetuses and newborn rats. In contrast, no effect was observed on cells from 17- and 19-day-old fetuses. At all stages investigated, the three doses of rANF were unable to affect ACTH-induced corticosterone secretion in vitro. In isolated adrenal cells from 2-week-old rats, rANF (10 nmol/l) inhibited the secretion of aldosterone induced by ACTH(1-24) (0.1 nmol/l), and by different steroids of the aldosterone synthetic pathway (progesterone, 11-deoxycorticosterone, corticosterone, 1 mumol/l for each steroid). These results suggest that rANF is a specific inhibitor of aldosterone synthesis in the perinatal period of the rat and that the inhibitory effect of rANF occurs both during the early and late pathways of aldosteroidogenesis.
authors.affiliation: Laboratoire de Neuroendocrinologie du Développement, Université des Sciences et Techniques, Villeneuve d'Ascq, France.
authors.forename: S
authors.initials: S
authors.lastname: Deloff
authors.forename: A
authors.initials: A
authors.lastname: Leprêtre
authors.forename: V
authors.initials: V
authors.lastname: Montel
authors.forename: A
authors.initials: A
authors.lastname: Châtelain
mesh_terms: D000311:Adrenal Glands / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D000324:Adrenocorticotropic Hormone / Q000494:pharmacology; D000450:Aldosterone / Q000097:blood / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn / Q000097:blood / Q000378:metabolism*; D009320:Atrial Natriuretic Factor / Q000494:pharmacology*; D003345:Corticosterone / Q000097:blood / Q000378:metabolism*; D005312:Fetal Blood; D005333:Fetus / Q000378:metabolism*; D000451:Mineralocorticoid Receptor Antagonists / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D011994:Recombinant Proteins
chemical_list: D000451:Mineralocorticoid Receptor Antagonists; D011994:Recombinant Proteins; C472610:atrial natriuretic peptide, rat; D000450:Aldosterone; D009320:Atrial Natriuretic Factor; D000324:Adrenocorticotropic Hormone; D003345:Corticosterone