title: Superoxide anion release from blood monocytes and alveolar macrophages in patients with diffuse lung fibrosis.
abstract: Superoxide anion release (O2-) after stimulation with phorbol myristate acetate was measured in alveolar macrophages (AM) obtained by bronchoalveolar lavage and in blood monocytes from 47 patients with diffuse interstitial lung disease: idiopathic pulmonary fibrosis (N = 15), hypersensitivity pneumonitis (N = 7), pneumoconiosis (N = 6) and sarcoidosis (N = 19). Differential cell counts demonstrated a lymphocyte predominance in patients with hypersensitivity pneumonitis (HP) and sarcoidosis while the other groups had neutrophil predominance. No correlation between O2- activity in alveolar macrophages (AM) or blood monocytes (BM) compared to lung function (VC and diffusing capacity) could be demonstrated. Smoking pneumoconiotics had significantly decreased BM O2- release (1.25 +/- 0.25 (SEM) nmol/min/10(6) cells) and significantly increased AM/BM O2- ratios (2.04 +/- 0.26) compared to smokers with idiopathic pulmonary fibrosis (IPF) who had the following mean values: BM O2- release = 2.58 +/- 0.25 and AM/BM O2- ratio = 0.86 +/- 0.23. When matched for sex and smoking, a significantly increased AM/BM O2- ratio was seen among patients with HP (2.19 +/- 0.98) in comparison with patients who had sarcoidosis (0.40 +/- 0.18). Patients with either HP or pneumoconiosis had generally elevated AM O2- release and reduced BM O2- release. These results suggest that environmentally related interstitial lung disorders (HP and pneumoconiosis) may be associated with elevated AM O2- release relative to BM O2- release in comparison to non-environmentally related disorders (IPF or sarcoidosis).
authors.affiliation: Department of Pulmonary Medicine, Bispebjerg Hospital, Copenhagen, Denmark.
authors.forename: D
authors.initials: D
authors.lastname: Sherson
authors.forename: H
authors.initials: H
authors.lastname: Nielsen
authors.forename: J
authors.initials: J
authors.lastname: Frederiksen
authors.forename: N
authors.initials: N
authors.lastname: Milman
authors.forename: E
authors.initials: E
authors.lastname: Struve-Christensen
authors.forename: B N
authors.initials: BN
authors.lastname: Petersen
mesh_terms: D000328:Adult; D000542:Alveolitis, Extrinsic Allergic / Q000097:blood / Q000378:metabolism / Q000503:physiopathology; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D002469:Cell Separation; D006801:Humans; D016676:Macrophages, Alveolar / Q000378:metabolism* / Q000473:pathology; D008875:Middle Aged; D009000:Monocytes / Q000378:metabolism* / Q000473:pathology; D011009:Pneumoconiosis / Q000097:blood / Q000378:metabolism / Q000503:physiopathology; D011658:Pulmonary Fibrosis / Q000097:blood / Q000378:metabolism* / Q000503:physiopathology; D012507:Sarcoidosis / Q000097:blood / Q000378:metabolism / Q000503:physiopathology; D013481:Superoxides / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
chemical_list: D013481:Superoxides; D013755:Tetradecanoylphorbol Acetate