Is it possible to use the hypoosmotic swelling test as criteria for "freezeability" of human semen in an AID program?
The aim of this study was to determine the possibility of the introduction of another parameter--the hypoosmotic swelling test--in the evaluation of the freezing potentially of anonymous donor's semen sample to be utilized in an AID program. 154 donor semen samples were frozen using classic seminal parameters: HOS tests were performed but not utilized as criteria for freezeability. All specimens were thawed and another HOS test was performed. All specimens were divided into two groups on the basis of the pre freezing HOS test value--HOS tests positive (> or = 55%) and HOS test negative (< 55%)--to verify if it's possible to correlate this value to the recovery of a good motility after thawing. A further division was performed: HOS tests highly positive (> or = 70%), HOS positive (60-55%) and HOS tests negative (< 55%) but the authors did not find any statistical difference. As concerns the clinical evaluation, were considered the last 15 pregnancies achieved with 11 samples out of 22 utilized. There was not any statistical difference. The data could seem to confirm the hypothesis that it is not possible to utilize the HOS test as a predictive value of "freezeability" of human semen sample.
Volpes
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Centro Andros Cecos Italia, Palermo.
Sammartano
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Coffaro
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F
Guida
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S
Scaglione
P
P
Allegra
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A
D015925:Cryopreservation; D005307:Fertilization in Vitro; D006801:Humans; D007038:Hypotonic Solutions; D007316:Insemination, Artificial, Heterologous; D008297:Male; D009995:Osmosis; D011237:Predictive Value of Tests; D012662:Semen Preservation; D013081:Sperm Motility; D013094:Spermatozoa / Q000502:physiology*
D007038:Hypotonic Solutions