Evaluation of desferal as a bifunctional chelating agent for labeling antibodies with Zr-89.
Zirconium-desferal was prepared and analysed by TLC, NMR and u.v.-spectroscopy. The stoichiometry of the complex was found to be 1:1. Chelation of desferal, coupled to resin, with 88Zr appeared to be fast and almost quantitative in various buffer systems in a broad pH-range (4-7). A high in vitro stability of the zirconium-desferal complex was observed; less than 0.2% zirconium was lost within 24 h in plasma-solutions.
Meijs
W E
WE
Radio-Nuclide-Centre (RNC), Free University, Amsterdam, The Netherlands.
Herscheid
J D
JD
Haisma
H J
HJ
Pinedo
H M
HM
D000906:Antibodies; D002614:Chelating Agents; D003676:Deferoxamine; D005069:Evaluation Studies as Topic; D007553:Isotope Labeling / Q000379:methods*; D011868:Radioisotopes; D015040:Zirconium
D000906:Antibodies; D002614:Chelating Agents; D011868:Radioisotopes; D015040:Zirconium; D003676:Deferoxamine