title: Comparison of herpesviruses isolated from reindeer, goats, and cattle by restriction endonuclease analysis.
abstract: A genomic comparison of bovine herpesvirus 1 (BHV-1), caprine herpesvirus (CHV-2) and reindeer herpesvirus (RHV), was performed using 5 restriction endonucleases. Cross neutralization of these three herpesviruses showed that BHV-1 and CHV-2 had a relatively low degree of cross reaction with heterologous viruses. RHV showed a higher degree of such cross reactivity. The restriction endonuclease analyses showed that the migration patterns of the DNA segments were different for the three groups of herpesviruses. The enteric caprine strain could be differentiated from genital strains using BstE II and Hpa I. The genome size of reindeer herpesvirus was estimated to be approximately 86.8 x 10(6) Da (131.8 kbp), and indications of isomerization of this genome were found. It is concluded that reindeer herpesvirus is a distinct species within the family Herpesviridae.
authors.affiliation: Department of Microbiology and Immunology, Norwegian College of Veterinary Medicine, Oslo.
authors.forename: E
authors.initials: E
authors.lastname: Rimstad
authors.forename: R
authors.initials: R
authors.lastname: Krona
authors.forename: B
authors.initials: B
authors.lastname: Hyllseth
mesh_terms: D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D003429:Cross Reactions; D004262:DNA Restriction Enzymes / Q000378:metabolism*; D005260:Female; D006041:Goats / Q000382:microbiology; D006564:Herpesviridae / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D006566:Herpesviridae Infections / Q000382:microbiology / Q000662:veterinary; D007242:Herpesvirus 1, Bovine / Q000235:genetics*; D008297:Male; D009500:Neutralization Tests; D012053:Reindeer / Q000382:microbiology
chemical_list: D004262:DNA Restriction Enzymes