Advanced glycosylation end products in the mesenteric artery.
We measured advanced glycosylation end products in the mesenteric artery of 37 patients (ages 29-82 years), 34 of whom were nondiabetic. Samples of arterial tissue were obtained during bowel resectioning. Advanced glycosylation end products were measured as collagen-linked fluorescence (excitation wavelength 370 nm, emission wavelength 440 nm) after collagenase digestion of tissue samples. Mean fluorescence of the arterial samples was 15 U/mg (range 5.3-27). Collagen fluorescence correlated with patients' age (r = 0.57; P less than 0.001). No difference in the collagen-linked fluorescence was observed between men and women (P = 0.63), hypertensive and normotensive patients (P = 0.44), smokers and nonsmokers (P = -0.52), and patients with and without symptomatic coronary heart disease (P = 0.7). This study demonstrates, for the first time, the relationship between collagen-linked fluorescence and patients' age in human arterial tissue ex vivo.
MacDonald
E
E
Department of Biochemistry, Western Infirmary, Glasgow, Scotland, U.K.
Lee
W K
WK
Hepburn
S
S
Bell
J
J
Scott
P J
PJ
Dominiczak
M H
MH
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000378:metabolism*; D003094:Collagen / Q000378:metabolism*; D003327:Coronary Disease / Q000378:metabolism; D005260:Female; D006031:Glycosylation; D006801:Humans; D006973:Hypertension / Q000378:metabolism; D015212:Inflammatory Bowel Diseases / Q000378:metabolism; D008297:Male; D008638:Mesenteric Arteries / Q000378:metabolism*; D003012:Microbial Collagenase / Q000378:metabolism; D008875:Middle Aged; D009369:Neoplasms / Q000378:metabolism; D013050:Spectrometry, Fluorescence
D003094:Collagen; D003012:Microbial Collagenase