Mouse peritoneal macrophage prostaglandin E1 synthesis is altered by dietary gamma-linolenic acid.
The ability of dietary gamma-linolenic acid [18:3(n-6)] to modulate prostaglandin biosynthesis in mouse resident peritoneal macrophages was determined. Mice were fed diets containing corn oil, borage oil or evening primrose oil or a mixture of borage and fish oils. After 2 wk, resident peritoneal macrophages were isolated and stimulated with unopsonized zymosan to induce prostaglandin synthesis. Borage oil, primrose oil and fish-borage oil mixture dietary groups (containing 25.6, 11.9 and 19.5 g gamma-linolenic acid/100 g fatty acids, respectively) had significantly (P less than 0.05) enhanced prostaglandin E1 synthesis (39.7, 29.4 and 73.0 nmol prostaglandin E1/mg protein, respectively) compared with corn oil-fed (containing less than 0.1 g gamma-linolenic acid/100 g fatty acids) animals, which synthesized less than 0.1 nmol prostaglandin E1/mg protein. Borage oil- and fish-borage oil mixture-fed mice had the highest biosynthetic ratio of prostaglandin E1/prostaglandin E2 (E1/E2 approximately 0.2). Macrophages from borage oil-fed mice synthesized the lowest amount of prostacyclin (198.7 nmol 6-keto-prostaglandin F1 alpha/mg protein) compared with corn oil-, primrose oil- and fish-borage oil mixture-fed mice (379.7, 764.8 and 384.2 nmol 6-keto-prostaglandin F1 alpha/mg protein, respectively). In addition, borage oil-, primrose oil- and fish-borage oil mixture-fed mice had significantly (P less than 0.05) higher levels of dihomo-gamma-linolenic acid [20:3(n-6)] in membrane phospholipids (5.5, 3.5 and 5.7 mol/100 mol, respectively) relative to corn oil-fed mice (2.0 mol/100 mol).(ABSTRACT TRUNCATED AT 250 WORDS)
Fan
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Department of Animal Science, Texas A&M University, College Station 77843-2471.
Chapkin
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D000527:Alprostadil / Q000096:biosynthesis; D000818:Animals; D001202:Ascitic Fluid / Q000166:cytology; D004042:Dietary Fats, Unsaturated / Q000008:administration & dosage / Q000494:pharmacology*; D015232:Dinoprostone / Q000096:biosynthesis; D011464:Epoprostenol / Q000096:biosynthesis; D005228:Fatty Acids, Essential / Q000494:pharmacology; D005260:Female; D005395:Fish Oils / Q000494:pharmacology; D008041:Linoleic Acids; D008042:Linolenic Acids / Q000008:administration & dosage / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D031665:Oenothera biennis; D010743:Phospholipids / Q000378:metabolism; D010938:Plant Oils / Q000494:pharmacology; D015054:Zymosan / Q000494:pharmacology; D017965:gamma-Linolenic Acid
D004042:Dietary Fats, Unsaturated; D005228:Fatty Acids, Essential; D005395:Fish Oils; D008041:Linoleic Acids; D008042:Linolenic Acids; D010743:Phospholipids; D010938:Plant Oils; C028498:evening primrose oil; D017965:gamma-Linolenic Acid; D015054:Zymosan; D011464:Epoprostenol; D000527:Alprostadil; D015232:Dinoprostone