Anti-phosphoinositide auto-antibodies in sera of cancer patients: isotypic and immunochemical characterization.
High levels of circulating anti-phosphatidylinositol (PtdIns) auto-antibodies (auto-Ab) have been previously found in sera of patients with malignant tumors. Using our ELISA test, a high statistical level of immunological binding directed against PtdIns was found in a large series of sera from patients with proliferative pathologies. In contrast to tumor markers, anti-PtdIns auto-Ab did not vary whatever the histological grades, TMN classification and patient's age. These anti-PtdIns auto-Ab were immunoglobulins (Ig) of G class. Their specificity was evaluated by competition experiments in ELISA and found to be rather high. An increase of these circulating auto-Ab reflected possible disturbances in PtdIns turnover and appearance of endogenous neo-antigen with PtdIns structure.
Faiderbe
S
S
Laboratoire d'Immunologie et Pathologie, Université de Bordeaux II, France.
Chagnaud
J L
JL
Geffard
M
M
D000912:Antibodies, Neoplasm / Q000097:blood* / Q000276:immunology / Q000378:metabolism; D000915:Antibody Affinity; D000918:Antibody Specificity; D001323:Autoantibodies / Q000097:blood* / Q000276:immunology / Q000378:metabolism; D001666:Binding Sites, Antibody; D004848:Epithelium / Q000473:pathology; D006402:Hematologic Diseases / Q000097:blood / Q000276:immunology / Q000473:pathology; D006801:Humans; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000097:blood; D007150:Immunohistochemistry; D009367:Neoplasm Staging; D009369:Neoplasms / Q000097:blood / Q000276:immunology* / Q000473:pathology; D010716:Phosphatidylinositols / Q000276:immunology*
D000912:Antibodies, Neoplasm; D001323:Autoantibodies; D007074:Immunoglobulin G; D010716:Phosphatidylinositols