[The spectral and paramagnetic properties of oxyhemoglobin solutions UV-irradiated in the presence of ascorbic acid].
Absorption spectra and ESR of aqueous and aqueous/glyceric solutions of oxyhemoglobin exposed to UV radiation (250-400 nm) at 293 and 77 K in the presence of ascorbic acid have been analyzed. Vitamin C (5 x 10(-5) M) has been shown to exert a photoprotective effect with regard to oxyhemoglobin (2 x 10(-6) M) UV-irradiated with a dose of 0.86 x 10(5) J/m2 at 293 K. The photoprotective effect of ascorbic acid is also displayed after UV irradiation of frozen (77 K) aqueous/glyceric oxyhemoglobin solutions (2.53 x 10(-5) M). It is concluded that ascorbic acid can be a scavenger with respect to active UV-induced particles in protein systems, including O2-. and OH. Proposed is a mode of processes leading to UV inactivation of hemoprotein molecules.
Artiukhov
V G
VG
D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology*; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004578:Electron Spin Resonance Spectroscopy; D005990:Glycerol; D051379:Mice; D010108:Oxyhemoglobins / Q000032:analysis / Q000187:drug effects / Q000528:radiation effects*; D012996:Solutions; D013054:Spectrophotometry, Atomic; D014466:Ultraviolet Rays; D014867:Water
D010108:Oxyhemoglobins; D012996:Solutions; D014867:Water; D005990:Glycerol; D001205:Ascorbic Acid
Spektral'nye i paramagnitnye svoĭstva rastvorov oksigemoglobina, UF-obluchennykh v prisutstvii askorbinovoĭ kisloty.