Cysteine protects Na,K-ATPase and isolated human lymphocytes from silver toxicity.
Metal-binding proteins are important components of retroviruses such as human immunodeficiency virus (HIV). Therefore, metals could be used as antiviral agents. However, most metals are toxic for humans with the exception of silver which is toxic only to prokaryotic cells and viruses. In addition, HIV infection causes a decrease in body cysteine. We formed a complex of silver and cysteine, named silver-cysteine. Healthy human lymphocytes were incubated with silver-nitrate or silver-cysteine. Negligible cell survival was seen at 50 microM silver-nitrate. However, in presence of 1 mM cysteine, the viability remained unaffected up to 1 mM of silver. Further, silver inhibition of isolated Na,K-ATPase was easily reversed by cysteine. Thus, non-toxic silver-cysteine could be used as an anti-viral and cysteine-replenishing agent.
Hussain
S
S
Laboratory of Experimental Therapeutics, Geneva University Medical Center, Switzerland.
Anner
R M
RM
Anner
B M
BM
D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D003545:Cysteine / Q000494:pharmacology*; D006678:HIV / Q000187:drug effects; D006801:Humans; D066298:In Vitro Techniques; D007679:Kidney Medulla / Q000201:enzymology; D007700:Kinetics; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects*; D012756:Sheep; D012834:Silver / Q000633:toxicity*; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors / Q000378:metabolism*
D012834:Silver; D000254:Sodium-Potassium-Exchanging ATPase; D003545:Cysteine