Axenic massive cultivation of Entamoeba histolytica trophozoites in spinner flasks and in a microfermentor.
A medium, PEHPS, whose main components are extracts of ox liver, and ox and swine pancreas (EHP) was used to develop two alternative methods for axenic cultivation of trophozoites in suspension: with magnetic stirrer and with a 14 l capacity microfermentor, giving the first technic yields of 2.36 x 10(5) to 3.08 x 10(5) and the second in a 14 l capacity microfermentor, which produced 1.65 x 10(5) amebas/ml in one batch and 2.94 to 3.65 x 10(5) of such parasites/ml at semicontinuous flux.
Said-Fernández
S
S
División de Biología Celular, Instituto Mexicano del Seguro Social, Monterrey, NL.
Mata-Cárdenas
B D
BD
Castro-Garza
J
J
Martínez-Rodríguez
H
H
Navarro-Marmolejo
L
L
Lozano-Garza
G
G
D000818:Animals; D003470:Culture Media; D004748:Entamoeba histolytica / Q000254:growth & development*; D005285:Fermentation; D008099:Liver; D010179:Pancreas; D010274:Parasitology / Q000295:instrumentation / Q000379:methods*; D013552:Swine; D014020:Tissue Extracts
D003470:Culture Media; D014020:Tissue Extracts