Beneficial effect of insulin on the decreased gastrointestinal beta-adrenergic responses in streptozotocin-diabetic rats: a contributing role of protein biosynthesis.
The beneficial effect of insulin on the decreased gastrointestinal beta-adrenergic responses in experimentally diabetic rats has been reported. The effects of streptozotocin-induced diabetes and insulin on the isolated rat duodenum, precontracted with bethanechol, were examined in the absence and in the presence of protein synthesis inhibitors in isometric conditions. Streptozotocin-induced diabetes caused a decrease of the beta-adrenergic responses of the rat duodenum. In vitro insulin treatment corrected the decreased beta-adrenergic responses of rat duodenum due to streptozotocin-induced diabetes. In the presence of protein synthesis inhibitors, actinomycin D or cycloheximide, the beneficial effect of insulin on the decreased intestinal beta-adrenergic responses was significantly inhibited. These findings suggest that the beneficial effect of insulin in the rat duodenum might be related to a new receptor synthesis.
Oztürk
Y
Y
Department of Pharmacology, Faculty of Pharmacy, Ankara University, Turkey.
Altan
V M
VM
Ozçelikay
A T
AT
Yildizoğlu-Ari
N
N
D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D001835:Body Weight / Q000187:drug effects; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D004064:Digestive System / Q000187:drug effects*; D004386:Duodenum / Q000187:drug effects; D066298:In Vitro Techniques; D007328:Insulin / Q000097:blood / Q000494:pharmacology*; D008297:Male; D014176:Protein Biosynthesis; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D011943:Receptors, Adrenergic, beta / Q000187:drug effects*
D001786:Blood Glucose; D007328:Insulin; D011500:Protein Synthesis Inhibitors; D011943:Receptors, Adrenergic, beta