Further physicochemical characterization of the monocyte locomotion inhibition factor (MLIF) produced by Entamoeba histolytica.
Ultrafiltration, gel-sieve chromatography and HPLC were used to purify MLIF. This material was HCl-hydrolyzed and the amino acids analyzed by HPLC-ortho-ophthaldialdehyde. Two hydrophobic non-polar (ile,leu), two uncharged polar (thr,tyr) and two positively charged (basic) (his, arg) amino acids were found in clear excess to their concentration in AMC and thus may intervene in the composition of MLIF.
Díaz-Guerra
O
O
División de Inmunología, Subjefatura de Investigación, CMN Siglo XXI, IMSS, México, DF.
Rico
G
G
Ladrón de Guevara
O
O
Padilla
P
P
García
L
L
Kretschmer
R R
RR
D000596:Amino Acids; D000818:Animals; D002634:Chemotaxis, Leukocyte / Q000187:drug effects*; D002851:Chromatography, High Pressure Liquid; D004748:Entamoeba histolytica / Q000737:chemistry*; D006801:Humans; D009000:Monocytes / Q000187:drug effects*; D015800:Protozoan Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology
D000596:Amino Acids; D015800:Protozoan Proteins