title: Peripheral nervous system demyelination from systemic transfer of experimental allergic neuritis serum.
abstract: The ability of systemically transferred experimental allergic neuritis (EAN) serum to produce EAN lesions in recipient animals was studied. Seventeen Lewis rats received five daily 1-ml intraperitoneal (i.p.) injections of sera from rabbits with EAN induced with bovine myelin/complete Freund's adjuvant (CFA). Another 17 rats received similar injections of sera from rabbits inoculated with CFA alone. On day 0 (the first day of i.p. injections), all rats were injected in the proximal tibial branch of the right sciatic nerve with a single 10-microliters injection of 0.03 M 5-hydroxytryptamine (5-HT) in sterile 0.15 M saline. Proximal tibial branches of left sciatic nerves received similar single injections of saline alone. Animals were then studied using electrophysiological and histological techniques. In all animals, intraneural saline injection had no significant effect upon nerve conduction. In the presence of circulating CFA serum, 5-HT injection caused a mild gradual decrease in amplitude ratio becoming maximal by day 17 (P < 0.005) and partially resolving by day 28. In contrast, in the presence of circulating EAN serum, 5-HT injection caused a more rapid and severe decrease in amplitude ratio becoming maximal by days 6-10 (P < 0.001 day 6; P < 0.0001 day 10) and completely resolving by day 28. Histological analysis of nerves injected with 5-HT in CFA serum-treated animals showed areas of mild demyelination, axonal degeneration and some fibre loss consistent with needle trauma. In contrast, 5-HT-injected nerves in animals administered EAN serum showed areas of marked cellular infiltration and severe demyelination in association with numerous debris-filled infiltrating cells.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Medicine, University of Sydney, Australia.
authors.forename: G K
authors.initials: GK
authors.lastname: Harvey
authors.forename: J D
authors.initials: JD
authors.lastname: Pollard
mesh_terms: D000818:Animals; D003711:Demyelinating Diseases / Q000209:etiology* / Q000503:physiopathology; D005260:Female; D005620:Freund's Adjuvant / Q000276:immunology; D008297:Male; D009431:Neural Conduction; D009444:Neuritis, Autoimmune, Experimental / Q000097:blood*; D010523:Peripheral Nervous System Diseases / Q000209:etiology* / Q000503:physiopathology; D011817:Rabbits; D051381:Rats; D011917:Rats, Inbred Lew; D012701:Serotonin / Q000494:pharmacology
chemical_list: D012701:Serotonin; D005620:Freund's Adjuvant