Effects of the Fab fragment of digoxin antibody on the natriuresis and increase in blood pressure induced by intracerebroventricular infusion of hypertonic saline solution in rats.
1. The effects of intravenous injection of Fab fragments of anti-digoxin IgG (Digibind) on the changes in blood pressure, urine volume and urinary sodium excretion after intracerebroventricular infusion of artificial cerebrospinal fluid with normal or high sodium concentration were examined in anaesthetized rats. 2. The biological efficacy of Digibind was confirmed by experiments in vitro and in vivo, which showed that pretreatment with Digibind completely abolished or significantly attenuated the aortic contractile response or pressor response to digoxin in guinea-pigs. 3. Infusion of high-sodium cerebrospinal fluid, but not normal-sodium cerebrospinal fluid, into the lateral brain ventricle of rats caused marked increases in blood pressure, urine volume and urinary sodium excretion. 4. Digibind did not significantly affect the increases in blood pressure, urine volume and urinary sodium excretion caused by intracerebroventricular infusion of high-sodium cerebrospinal fluid. 5. Digoxin-like immunoreactive factor may play a minor role, if any, in central nervous system-induced natriuresis in rats.
Yamada
K
K
Second Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Japan.
Goto
A
A
Hui
C
C
Yagi
N
N
Sugimoto
T
T
D000818:Animals; D001013:Aorta, Thoracic / Q000187:drug effects; D001794:Blood Pressure / Q000187:drug effects / Q000502:physiology*; D004077:Digoxin / Q000276:immunology* / Q000494:pharmacology; D007140:Immunoglobulin Fab Fragments / Q000008:administration & dosage* / Q000276:immunology; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects; D009318:Natriuresis / Q000502:physiology*; D051381:Rats; D011919:Rats, Inbred Strains; D012462:Saline Solution, Hypertonic / Q000494:pharmacology; D012964:Sodium / Q000652:urine
D007140:Immunoglobulin Fab Fragments; D012462:Saline Solution, Hypertonic; C050199:digoxin antibodies Fab fragments; D004077:Digoxin; D012964:Sodium