Biological activities of recombinant murine interferons alpha 1 and alpha 4: large difference in antiproliferative effect.
The mature forms of two recombinant murine interferons alpha, alpha 1 and alpha 4, have been expressed in vitro using an established transcription and translation system. The relative specific antiviral activity, antiproliferative activity and the natural killer cell stimulating activity of both subtypes were compared in vitro. While the antiviral and natural killer cell stimulating activities of the 2 subtypes were similar, the relative antiproliferative activities varied markedly. On the basis of equal molar inputs, MuIFN-alpha 1 had less than 8% of the antiproliferative activity of MuIFN-alpha 4. This data shows that a large functional difference exists between these two subtypes which are known to be expressed at different levels in mouse L-cells in vitro.
Swaminathan
N
N
Department of Microbiology, University of Western Australia, Nedlands.
Lai
C M
CM
Beilharz
M W
MW
Boyer
S J
SJ
Klinken
S P
SP
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D004686:Encephalomyocarditis virus / Q000187:drug effects; D006131:Growth Inhibitors / Q000494:pharmacology*; D007370:Interferon Type I / Q000145:classification / Q000494:pharmacology*; D007694:Killer Cells, Natural / Q000187:drug effects; D007739:L Cells / Q000187:drug effects; D004915:Leukemia, Erythroblastic, Acute / Q000188:drug therapy / Q000473:pathology; D051379:Mice; D008809:Mice, Inbred C3H; D011994:Recombinant Proteins; D014407:Tumor Cells, Cultured; D014779:Virus Replication / Q000187:drug effects
D006131:Growth Inhibitors; D007370:Interferon Type I; D011994:Recombinant Proteins