title: Competitive adsorption of human immunoglobulin G and albumin: consequences for structure and reactivity of the adsorbed layer.
abstract: The affinity of polyclonal anti-IgG for human IgG adsorbed on silica surfaces was investigated by two complementary techniques, scanning angle reflectometry and 125I radiotracing. Special attention was paid to compare the reactivity of IgG adsorbed directly or by exchange with already adsorbed albumin. In particular it was shown that (i) in the first case (direct adsorption) the reaction between anti-IgG and adsorbed IgG was in the ratio 1:1 and (ii) in the second case (adsorption by exchange) there was no reaction.
authors.affiliation: Centre de Recherches Odontologiques, Institut National de la Santé et de la Recherche Médicale, Université Louis Pasteur.
authors.forename: E
authors.initials: E
authors.lastname: Lutanie
authors.forename: J C
authors.initials: JC
authors.lastname: Voegel
authors.forename: P
authors.initials: P
authors.lastname: Schaaf
authors.forename: M
authors.initials: M
authors.lastname: Freund
authors.forename: J P
authors.initials: JP
authors.lastname: Cazenave
authors.forename: A
authors.initials: A
authors.lastname: Schmitt
mesh_terms: D000327:Adsorption; D000418:Albumins / Q000737:chemistry*; D000818:Animals; D002645:Chickens; D006801:Humans; D007074:Immunoglobulin G / Q000737:chemistry*; D007136:Immunoglobulins / Q000737:chemistry; D066298:In Vitro Techniques; D012822:Silicon Dioxide / Q000737:chemistry
chemical_list: D000418:Albumins; C016512:IgY; D007074:Immunoglobulin G; D007136:Immunoglobulins; D012822:Silicon Dioxide