Transformation of human colostrum lymphocytes with Epstein-Barr virus.
Lymphocytes from human colostrum were transformed with EB virus to obtain immunoglobulin secreting cells. Equal proportions of IgG, IgA and IgM were detected in wells containing transformed colostrum cells. Pretreatment of colostrum cells with BCGF prior to EB virus infection reduced the transformation slightly. We discussed the possibility of establishing cell lines that produced human monoclonal antibody using colostrum cells.
Shinmoto
H
H
Technical Research Institute, Snow Brand Milk Products Co., Ltd., Kawagoe, Japan.
Dosako
S
S
Tanaka
S
S
D002472:Cell Transformation, Viral; D003126:Colostrum / Q000166:cytology* / Q000276:immunology*; D005260:Female; D004854:Herpesvirus 4, Human; D006801:Humans; D007071:Immunoglobulin A, Secretory / Q000096:biosynthesis; D007074:Immunoglobulin G / Q000096:biosynthesis; D007075:Immunoglobulin M / Q000096:biosynthesis; D015847:Interleukin-4 / Q000494:pharmacology; D008214:Lymphocytes / Q000276:immunology*
D007071:Immunoglobulin A, Secretory; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D015847:Interleukin-4