The effect of anion transport inhibitors and extracellular Cl- concentration on eosinophil respiratory burst activity.
Furosemide has been shown recently to protect asthmatic patients against certain bronchoconstrictor challenges. We investigated the effect of furosemide on eosinophil function. Since furosemide may be exerting its inhibitory effect on the eosinophil by inhibiting anion transport, we also assessed the effects of the anion transport inhibitors 5-nitro-2-(3-phenylpropylamino)-benzoic acid (NPPB) and 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid (DIDS). Furosemide, NPPB and DIDS inhibited the eosinophil respiratory burst in response to leukotriene B4 (LTB4) and, to a smaller extent, inhibited the response to opsonized zymosan (OZ). To assess whether the anion transport inhibitors were achieving their inhibitory effect by inhibiting an influx of Cl- ions into the eosinophil, the effect of removing extracellular Cl- on eosinophil function was determined. OZ-induced H2O2 production was inhibited by removing extracellular Cl- whereas the LTB4 response was not affected by the concentration of extracellular Cl-.
Perkins
R S
RS
Department of Thoracic Medicine, National Heart and Lung Institute, London, U.K.
Dent
G
G
Chung
K F
KF
Barnes
P J
PJ
D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002712:Chlorides / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004804:Eosinophils / Q000187:drug effects* / Q000378:metabolism; D005665:Furosemide / Q000494:pharmacology*; D006168:Guinea Pigs; D006861:Hydrogen Peroxide / Q000032:analysis; D007975:Leukotriene B4; D009579:Nitrobenzoates / Q000494:pharmacology*; D016897:Respiratory Burst / Q000187:drug effects*; D015054:Zymosan
D002712:Chlorides; D009579:Nitrobenzoates; D007975:Leukotriene B4; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid; C058176:5-nitro-2-(3-phenylpropylamino)benzoic acid; D005665:Furosemide; D015054:Zymosan; D006861:Hydrogen Peroxide; D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid