Lipid peroxidation in experimental uveitis: sequential studies.
Previously we have detected the occurrence of retinal lipid peroxidation initiated by phagocyte-derived oxygen radicals in experimental autoimmune uveitis (EAU). In the current studies, the confirmation of inflammation-mediated lipid peroxidation was proceeded further to include measurement of multiple parameters, including conjugated dienes, ketodienes, thiobarbituric acid reactive substances and fluorescent chromolipids. The assay for myeloperoxidase, a measure for the number of polymorphonuclear leukocytes in the inflammatory sites was also carried out. The levels of all these parameters were followed through the course of EAU development. The sequential evaluation of histologic changes using both light and electron microscopy was also carried out and the results were correlated with lipid peroxidation indices. These data suggest that the retinal lipid peroxidation plays a causative role in the subsequent retinal degeneration.
Goto
H
H
Doheny Eye Institute, Department of Ophthalmology, USC School of Medicine, Los Angeles 90033.
Wu
G S
GS
Chen
F
F
Kristeva
M
M
Sevanian
A
A
Rao
N A
NA
D000818:Animals; D000941:Antigens; D019393:Arrestin; D001327:Autoimmune Diseases / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D002829:Choroid / Q000648:ultrastructure; D004195:Disease Models, Animal; D005136:Eye Proteins; D005260:Female; D015227:Lipid Peroxidation / Q000276:immunology* / Q000502:physiology; D008565:Membrane Proteins / Q000276:immunology; D009195:Peroxidase / Q000378:metabolism; D010726:Phosphodiesterase Inhibitors; D051381:Rats; D011917:Rats, Inbred Lew; D012160:Retina / Q000201:enzymology / Q000276:immunology / Q000648:ultrastructure; D014605:Uveitis / Q000276:immunology* / Q000378:metabolism / Q000473:pathology
D000941:Antigens; D019393:Arrestin; D005136:Eye Proteins; D008565:Membrane Proteins; D010726:Phosphodiesterase Inhibitors; D009195:Peroxidase