Glycogen synthase kinase-3 and the Alzheimer-like state of microtubule-associated protein tau.
The Alzheimer-like state of tau protein includes phosphorylation by a proline-directed Ser/Thr kinase present in normal or pathological human brain. Extending earlier results on MAP kinase, we show here that the proline-directed kinase, GSK3, can induce an Alzheimer-like immune response involving several distinct and phosphorylatable epitopes at Ser-Pro motifs, as well as a gel mobility shift, similar to MAP kinase. Both kinases behave like microtubule-associated proteins in that they co-purify through cycles of assembly and disassembly, and both kinases are directly associated with paired helical filaments.
Mandelkow
E M
EM
Max-Planck-Unit for Structural Molecular Biology, DESY, Hamburg, Germany.
Drewes
G
G
Biernat
J
J
Gustke
N
N
Van Lint
J
J
Vandenheede
J R
JR
Mandelkow
E
E
D000544:Alzheimer Disease / Q000201:enzymology* / Q000473:pathology; D000818:Animals; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D002417:Cattle; D003001:Cloning, Molecular; D038341:Glycogen Synthase Kinases; D006801:Humans; D007382:Intermediate Filaments / Q000378:metabolism; D007700:Kinetics; D008870:Microtubules / Q000378:metabolism; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism*; D016875:tau Proteins / Q000737:chemistry / Q000378:metabolism*
D016875:tau Proteins; D011494:Protein Kinases; D038341:Glycogen Synthase Kinases; D017871:Calcium-Calmodulin-Dependent Protein Kinases