title: Modified binary plant transformation vectors with the wild-type gene encoding NPTII.
abstract: The defective gene encoding neomycin phosphotransferase (NPTII) present in the binary plasmid vector, pBin19, was replaced with the wild-type (wt) gene. Plasmid vectors analogous to pBin19, pBI121 and pBI101 were constructed carrying the gene encoding the wt NPTII enzyme activity.
authors.affiliation: Plant Biotechnology Institute, National Research Council of Canada, Saskatoon, Sask.
authors.forename: R S
authors.initials: RS
authors.lastname: Datla
authors.forename: J K
authors.initials: JK
authors.lastname: Hammerlindl
authors.forename: B
authors.initials: B
authors.lastname: Panchuk
authors.forename: L E
authors.initials: LE
authors.lastname: Pelcher
authors.forename: W
authors.initials: W
authors.lastname: Keller
mesh_terms: D005822:Genetic Vectors; D019868:Kanamycin Kinase; D010770:Phosphotransferases / Q000235:genetics*; D010944:Plants / Q000235:genetics*; D010957:Plasmids; D014170:Transformation, Genetic
chemical_list: D010770:Phosphotransferases; D019868:Kanamycin Kinase