title: Detection and typing of human papillomaviruses present in fixed and stained archival cervical smears by a consensus polymerase chain reaction and direct sequence analysis allow the identification of a broad spectrum of human papillomavirus types.
abstract: DNA well suited for polymerase chain reaction (PCR) amplification was purified from archival Papanicolaou smears. The detection of a wide range of human papillomavirus (HPV) types was made possible using a HPV-specific consensus primer pair, and typing was conveniently done by direct sequence analysis of the PCR product. The method could be of unique value in longitudinal and cross-sectional studies aimed at answering a number of fundamental pathological and epidemiological questions regarding HPV infection of the genital tract.
authors.affiliation: Department of Virology, Section of Fundamental Virology), University of Amsterdam, The Netherlands.
authors.forename: H L
authors.initials: HL
authors.lastname: Smits
authors.forename: L M
authors.initials: LM
authors.lastname: Tieben
authors.forename: S P
authors.initials: SP
authors.lastname: Tjong-A-Hung
authors.forename: M F
authors.initials: MF
authors.lastname: Jebbink
authors.forename: R P
authors.initials: RP
authors.lastname: Minnaar
authors.forename: C L
authors.initials: CL
authors.lastname: Jansen
authors.forename: J
authors.initials: J
authors.lastname: ter Schegget
mesh_terms: D001483:Base Sequence; D002584:Cervix Uteri / Q000382:microbiology; D004279:DNA, Viral / Q000032:analysis* / Q000235:genetics; D005260:Female; D006801:Humans; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D065006:Papanicolaou Test; D027383:Papillomaviridae / Q000145:classification / Q000235:genetics*; D016133:Polymerase Chain Reaction; D014412:Tumor Virus Infections / Q000175:diagnosis* / Q000235:genetics; D014626:Vaginal Smears
chemical_list: D004279:DNA, Viral; D009838:Oligodeoxyribonucleotides