Improved method for bile collection in unrestrained conscious rats.
We describe an improved method for continuous collection of bile from unrestrained rats. Use of an externally accessible, continuous-loop cannula when cannulating the common bile duct allows for full recovery from anesthetic effects and maintenance of a normal bile salt pool until the cannula loop is cut. Bile resulting from the cut cannula is diverted into a surgically implanted glass collection vessel and removed periodically via an externalized sampling port. Bile flow over a 24-hour collection period averaged 0.98 +/- 0.04 ml/hr (Mean +/- SEM, n = 9) with no gross pathological changes noted upon necropsy. This technique offers the capability of reestablishing conditions as close to physiologic as possible postsurgery to minimize potential artifacts during bile collection.
Heitmeyer
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SA
Procter & Gamble Company, Miami Valley Laboratories, Cincinnati, Ohio 45239-8707.
Powers
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JF
D000818:Animals; D001646:Bile; D002404:Catheterization / Q000295:instrumentation / Q000662:veterinary*; D008297:Male; D051381:Rats; D013048:Specimen Handling / Q000295:instrumentation / Q000379:methods / Q000662:veterinary*