Demonstration of the distribution of coxsackie virus RNA in neonatal mice by non-isotopic in situ hybridization.
A simple method for the demonstration of Coxsackie virus RNA by in situ hybridization is described. Oligonucleotides complimentary to conserved sequences of Coxsackie B genome were synthesised and labelled with digoxigenin using commercially available reagents. In addition to detecting all five Coxsackie B strains examined, six strains of Coxsackie A were also demonstrated by these probes. Using one of the oligonucleotides separately it was possible to distinguish Coxsackie A strains from the strains of Coxsackie B virus examined. This study demonstrates the presence of viral RNA in mice tissues showing morphological evidence of damage, confirming the suspected tropisms of these viruses. The method described is directly applicable to the study of the presence of these viruses in human tissue from diseases where a viral aetiology is suspected.
Hilton
D A
DA
Department of Pathology, Leicester Royal Infirmary, UK.
Day
C
C
Pringle
J H
JH
Fletcher
A
A
Chambers
S
S
D000818:Animals; D000831:Animals, Newborn; D001483:Base Sequence; D003384:Coxsackievirus Infections / Q000382:microbiology*; D004770:Enterovirus / Q000235:genetics / Q000302:isolation & purification*; D029822:Enterovirus B, Human / Q000235:genetics / Q000302:isolation & purification*; D017403:In Situ Hybridization; D051379:Mice; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D012367:RNA, Viral / Q000032:analysis*; D013047:Specific Pathogen-Free Organisms
D015345:Oligonucleotide Probes; D012367:RNA, Viral