Cyclic AMP-dependent increase of steroidogenesis in brain cortical minces.
Rat brain cortical minces were incubated with forskolin and dibutyryl-cyclic AMP for 60 and 30 min, respectively. The concentrations of pregnenolone, progesterone and desoxycorticosterone in this preparation were significantly increased by both substances. The results indicate that, in brain tissue, steroidogenesis appears to be regulated by receptor transduction mechanisms that operate through adenylate cyclase.
Barbaccia
M L
ML
Department of Experimental Medicine, University of Rome Tor Vergata, Italy.
Roscetti
G
G
Trabucchi
M
M
Ambrosio
C
C
Massotti
M
M
D000818:Animals; D002540:Cerebral Cortex / Q000187:drug effects* / Q000378:metabolism*; D005576:Colforsin / Q000494:pharmacology; D000242:Cyclic AMP / Q000502:physiology*; D003900:Desoxycorticosterone / Q000096:biosynthesis*; D066298:In Vitro Techniques; D008297:Male; D011284:Pregnenolone / Q000096:biosynthesis*; D011374:Progesterone / Q000096:biosynthesis*; D051381:Rats; D017207:Rats, Sprague-Dawley
D005576:Colforsin; D003900:Desoxycorticosterone; D011374:Progesterone; D011284:Pregnenolone; D000242:Cyclic AMP