[The polymerase chain reaction (PCR) for the detection of DNA of equine herpesviruses 1 and 4].
Formalin-fixed and Paraplast-embedded tissue samples of 42 aborted equine fetuses were examined by polymerase chain reaction for the presence of equine herpesvirus DNA. The used set of primers was located in the glycoprotein 13 open reading frame and allowed the amplification of both EHV 1 und EHV 4. By cleaving pattern analysis after Hinf I digestion EHV 1 could be distinguished from EHV 4. In 9 of the cases investigated EHV 1-DNA was detected. This finding is in absolute context with the results of the virological investigations.
Hardt
M
M
Institut für Veterinär-Pathologie, Justus-Liebig-Universität Giessen.
Teifke
J P
JP
Weiss
E
E
D000034:Abortion, Veterinary / Q000382:microbiology; D000818:Animals; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D006564:Herpesviridae / Q000235:genetics*; D006566:Herpesviridae Infections / Q000382:microbiology / Q000662:veterinary*; D004861:Herpesvirus 1, Equid / Q000235:genetics*; D006734:Horse Diseases / Q000382:microbiology*; D006736:Horses; D016133:Polymerase Chain Reaction; D011247:Pregnancy
D004279:DNA, Viral
Die Polymerasekettenreaktion (PCR) zum Nachweis von DNA der Equinen Herpesviren 1 und 4.