title: Calcium-activated currents in cultured neurones from rat dorsal root ganglia.
abstract: 1. Voltage-activated Ca2+ currents and caffeine (1 to 10 mM) were used to increase intracellular Ca2+ in rat cultured dorsal root ganglia (DRG) neurones. Elevation of intracellular Ca2+ resulted in activation of inward currents which were attenuated by increasing the Ca2+ buffering capacity of cells by raising the concentration of EGTA in the patch solution to 10 mM. Low and high voltage-activated Ca2+ currents gave rise to Cl- tail currents in cells loaded with CsCl patch solution. Outward Ca2+ channel currents activated at very depolarized potentials (Vc + 60 mV to + 100 mV) also activated Cl- tail currents, which were enhanced when extracellular Ca2+ was elevated from 2 mM to 4 mM. 2. The Ca(2+)-activated Cl- tail currents were identified by estimation of tail current reversal potential by use of a double pulse protocol and by sensitivity to the Cl- channel blocker 5-nitro 2-(3-phenyl-propylamino) benzoic acid (NPPB) applied at a concentration of 10 microM. 3. Cells loaded with Cs acetate patch solution and bathed in medium containing 4 mM Ca2+ also had prolonged Ca(2+)-dependent tail currents, however these smaller tail currents were insensitive to NPPB. Release of Ca2+ from intracellular stores by caffeine gave rise to sustained inward currents in cells loaded with Cs acetate. Both Ca(2+)-activated tail currents and caffeine-induced inward currents recorded from cells loaded with Cs acetate were attenuated by Tris based recording media, and had reversal potentials positive to 0 mV suggesting activity of Ca(2+)-activated cation channels.4. Our data may reflect (a) different degrees of association between Ca2+-activated channels with voltage-gated Ca2+ channels, (b) distinct relationships between channels and intracellular Ca2" stores and Ca2+ homeostatic mechanisms, (c) regulation of Ca2+-activated channels by second messengers, and (d) varying channel sensitivity to Ca2 , in the cell body of DRG neurones.
authors.affiliation: Department of Physiology, St. George's Hospital Medical School, London.
authors.forename: K P
authors.initials: KP
authors.lastname: Currie
authors.forename: R H
authors.initials: RH
authors.lastname: Scott
mesh_terms: D000200:Action Potentials; D000818:Animals; D002110:Caffeine / Q000494:pharmacology; D002118:Calcium / Q000494:pharmacology / Q000502:physiology*; D015220:Calcium Channels / Q000187:drug effects; D002478:Cells, Cultured; D004566:Electrodes; D004594:Electrophysiology; D005727:Ganglia, Spinal / Q000502:physiology*; D009474:Neurons / Q000187:drug effects / Q000502:physiology; D051381:Rats
chemical_list: D015220:Calcium Channels; D002110:Caffeine; D002118:Calcium