The Ku complex is modulated in response to viral infection and other cellular changes.
The complex of Ku with DNA is demonstrated to have multiple forms as assayed by gel retardation analysis. In CV1 cells this variation of complex can be modulated in response to viral infection with SV40. By Western blot analysis, a correlation can be made between modification of the complex formed on DNA in response to viral infection with variation of the 85 kDa subunit of Ku. Modification of the 85 kDa subunit can also be seen when cells are exposed to various extracellular stimuli including variation in serum levels, PMA and CaPO4.
Quinn
J P
JP
MRC Brain Metabolism Unit, Royal Edinburgh Hospital, UK.
Simpson
J
J
Farina
A R
AR
D000595:Amino Acid Sequence; D000906:Antibodies / Q000276:immunology; D034961:Antigens, Nuclear; D001483:Base Sequence; D015153:Blotting, Western; D002460:Cell Line; D004247:DNA; D004265:DNA Helicases; D004268:DNA-Binding Proteins / Q000276:immunology / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D000072200:Ku Autoantigen; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000276:immunology / Q000378:metabolism*; D016755:Proto-Oncogene Proteins c-jun / Q000276:immunology; D013539:Simian virus 40 / Q000502:physiology*; D014779:Virus Replication
D000906:Antibodies; D034961:Antigens, Nuclear; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D016755:Proto-Oncogene Proteins c-jun; D004247:DNA; D004265:DNA Helicases; C106893:XRCC5 protein, human; C000606509:Xrcc6 protein, human; D000072200:Ku Autoantigen