Rainbow trout p53: cDNA cloning and biochemical characterization.
We have cloned and sequenced the p53-encoding cDNA of rainbow trout (Salmo gairdneri). The encoded product contains the characteristics found in all p53 proteins: (i) the five highly conserved domains, (ii) an acidic N terminus, (iii) a hydrophilic C terminus, and (iv) a penultimate serine residue. Furthermore, we demonstrate that the rainbow trout p53 is able to specifically interact with the SV40 large T antigen.
Caron de Fromentel
C
C
Unité d'Oncologie Moléculaire, IRSC, Villejuif, France.
Pakdel
F
F
Chapus
A
A
Baney
C
C
May
P
P
Soussi
T
T
D000595:Amino Acid Sequence; D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000378:metabolism; D001483:Base Sequence; D005075:Biological Evolution; D003001:Cloning, Molecular; D015342:DNA Probes / Q000235:genetics; D016158:Genes, p53 / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D014337:Trout / Q000235:genetics*; D016159:Tumor Suppressor Protein p53 / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
D000952:Antigens, Polyomavirus Transforming; D015342:DNA Probes; D016159:Tumor Suppressor Protein p53