Using restriction enzymes that cleave DNA outside of their recognition sequences to produce desired sticky ends.
Cohen
R S
RS
Dept. of Biochemistry and Molecular Biophysics, College of Physicians and Surgeons, Columbia University, New York, NY 10032.
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes / Q000378:metabolism*; D004274:DNA, Recombinant / Q000378:metabolism*; D004330:Drosophila / Q000235:genetics; D008969:Molecular Sequence Data; D013379:Substrate Specificity
D004274:DNA, Recombinant; D004262:DNA Restriction Enzymes