Chromatin structure, DNA methylation, and gene expression at sites of viral integration in human fibroblasts. Implications for chromosomal fragility.
We analyzed the structural and functional properties of a chromosomal region in which a recombinant hybrid virus adenovirus 5/SV40 preferentially integrates. Our results demonstrated that the structure of the cellular targets for DNA and RNA viruses is very similar and that the cellular sequence flanking the integrated virus possesses, simultaneously, all the features postulated to be the molecular basis for chromosomal fragility.
De Ambrosis
A
A
Laboratory of Molecular Biology, Istituto Nazionale per la Ricerca sul Cancro, Genova, Italy.
Casciano
I
I
Querzola
F
F
Vidali
G
G
Romani
M
M
D000256:Adenoviridae / Q000235:genetics*; D015152:Blotting, Northern; D015139:Blotting, Southern; D002843:Chromatin / Q000648:ultrastructure*; D004247:DNA / Q000378:metabolism*; D003850:Deoxyribonuclease I; D005347:Fibroblasts / Q000382:microbiology* / Q000648:ultrastructure; D015870:Gene Expression; D006801:Humans; D007438:Introns; D008745:Methylation; D011995:Recombination, Genetic; D015183:Restriction Mapping; D013539:Simian virus 40 / Q000235:genetics*; D015719:Single-Strand Specific DNA and RNA Endonucleases
D002843:Chromatin; D004247:DNA; D003850:Deoxyribonuclease I; D015719:Single-Strand Specific DNA and RNA Endonucleases