Expression of myogenic factors in skeletal muscle and electric organ of Torpedo californica.
Fish electric organ is a skeletal muscle homolog in which many muscle-specific genes are inhibited while acetylcholine receptor is expressed at high levels. The molecular mechanisms underlying this discoordinate regulation have not yet been explored. We have obtained partial sequences for MyoD, myogenin, and myf5 from Torpedo californica and have measured their mRNAs in several organs, using ribonuclease protection. We have found that MyoD and myf5 are expressed at comparable levels in muscle and electric organ, whereas myogenin transcripts could not be detected in either tissue. Acetylcholine receptor alpha subunit mRNA, on the other hand, is two orders of magnitude more abundant in electric tissue. We conclude that neither the loss of contractile proteins from, nor the enhanced expression of acetylcholine receptor genes in, the differentiating electrocyte is a simple consequence of the abundance of myogenic factor messages.
Neville
C M
CM
Department of Biochemistry and Cell Biology, State University of New York, Stony Brook 11794.
Schmidt
J
J
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004247:DNA; D004268:DNA-Binding Proteins; D004557:Electric Organ / Q000378:metabolism*; D008969:Molecular Sequence Data; D009124:Muscle Proteins / Q000096:biosynthesis*; D009132:Muscles / Q000378:metabolism*; D017570:MyoD Protein; D051787:Myogenic Regulatory Factor 5; D018008:Myogenin; D009928:Organ Specificity; D012333:RNA, Messenger / Q000235:genetics; D016415:Sequence Alignment; D014101:Torpedo; D015534:Trans-Activators
D004268:DNA-Binding Proteins; D009124:Muscle Proteins; D017570:MyoD Protein; D051787:Myogenic Regulatory Factor 5; D018008:Myogenin; D012333:RNA, Messenger; D015534:Trans-Activators; D004247:DNA