title: Hepatic uptake of a modified low molecular weight heparin in rats.
abstract: Fractionated and unfractionated heparins are widely used as antithrombotic agents. Because of their heterogeneous composition, it is difficult to study the pharmacokinetics of these drugs. We now report on a new method for labeling low molecular weight heparins with 131I by binding tyramine to the anhydromannose end of the molecules. We examined the pharmacokinetics of the compound by intravenous injection of 131I-tyramine-heparin into Wistar rats. About 18% of the activity was found in the liver, whereas 33% was detected in urine. Biological activity in terms of Factor Xa inhibition was measurable. Since evidence from cell culture experiments implies that reticuloendothelial cell system receptors might be involved in heparin metabolism, maleylated BSA, a substance known to block scavenger receptors, was injected before the radiolabeled heparin compound. The liver uptake was reduced from 17.4 to 4.8%. Injection of unfractionated heparin before tracer application caused a considerable increase in urine excretion of the tracer substance. To our knowledge, this is the first report that liver uptake of heparins is linked to scavenger receptor mediated mechanisms in vivo. This interaction of heparins with scavenger receptors might play an important role in the biology of the vessel wall.
authors.affiliation: First Department of Medicine, Faculty for Clinical Medicine Mannheim, University of Heidelberg, Germany.
authors.forename: G
authors.initials: G
authors.lastname: Stehle
authors.forename: E A
authors.initials: EA
authors.lastname: Friedrich
authors.forename: H
authors.initials: H
authors.lastname: Sinn
authors.forename: A
authors.initials: A
authors.lastname: Wunder
authors.forename: J
authors.initials: J
authors.lastname: Harenberg
authors.forename: C E
authors.initials: CE
authors.lastname: Dempfle
authors.forename: W
authors.initials: W
authors.lastname: Maier-Borst
authors.forename: D L
authors.initials: DL
authors.lastname: Heene
mesh_terms: D000818:Animals; D005260:Female; D006495:Heparin, Low-Molecular-Weight / Q000031:analogs & derivatives / Q000493:pharmacokinetics*; D008099:Liver / Q000378:metabolism*; D008565:Membrane Proteins; D008970:Molecular Weight; D051381:Rats; D017208:Rats, Wistar; D011971:Receptors, Immunologic / Q000502:physiology; D018110:Receptors, Lipoprotein; D051116:Receptors, Scavenger; D051122:Scavenger Receptors, Class B; D014018:Tissue Distribution
chemical_list: D006495:Heparin, Low-Molecular-Weight; D008565:Membrane Proteins; D011971:Receptors, Immunologic; D018110:Receptors, Lipoprotein; D051116:Receptors, Scavenger; C454739:Scarb1 protein, mouse; D051122:Scavenger Receptors, Class B