Loss of V protein expression in human parainfluenza virus type 1 is not a recent event.
The P gene of paramyxoviruses usually contains an alternate overlapping ORF coding for a short cysteine-rich domain called V. This domain was thought to be a common feature of these genes as it is present in ten paramyxoviruses. However, the V ORF region of the P gene of one strain of human parainfluenza-virus type 1 (isolated in 1957) was recently found to be closed by no less than 9 stop codons. To determine whether the absence of the V ORF here might be due to the long adaptation of this strain in culture, 3 other more recent isolates were examined. Small differences in the V region were found, but all had conserved the vast majority of the stop codons. Moreover, examination of most of the intercistronic regions of the PIV1 genome failed to uncover a cryptic V gene. The V ORF is then unlikely to be a common feature of paramyxovirus P genes.
Rochat
S
S
Department of Genetics and Microbiology, University of Geneva School of Medicine, Switzerland.
Komada
H
H
Kolakofsky
D
D
D000595:Amino Acid Sequence; D001483:Base Sequence; D015967:Gene Expression Regulation, Viral; D008969:Molecular Sequence Data; D010222:Parainfluenza Virus 1, Human / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics*
C068854:V protein, human parainfluenza virus type 1; D014764:Viral Proteins