Molecular cloning of osteoma-inducing replication-competent murine leukemia viruses from the RFB osteoma virus stock.
We report the molecular cloning of two replication-competent osteoma-inducing murine leukemia viruses from the RFB osteoma virus stock (M. P. Finkel, C. A. Reilly, Jr., B. O. Biskis, and I. L. Greco, p. 353-366, in C. H. G. Price and F. G. M. Ross, ed., Bone--Certain Aspects of Neoplasia, 1973). Like the original RFB osteoma virus stock, viruses derived from the molecular RFB clones induced multiple osteomas in mice of the CBA/Ca strain. The cloned RFB viruses were indistinguishable by restriction enzyme analysis and by nucleotide sequence analysis of their long-terminal-repeat regions and showed close relatedness to the Akv murine leukemia virus.
Pedersen
L
L
Department of Molecular Biology, University of Aarhus, Denmark.
Behnisch
W
W
Schmidt
J
J
Luz
A
A
Pedersen
F S
FS
Erfle
V
V
Strauss
P G
PG
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004279:DNA, Viral; D009052:Leukemia Virus, Murine / Q000235:genetics* / Q000472:pathogenicity / Q000502:physiology; D051379:Mice; D008808:Mice, Inbred CBA; D008969:Molecular Sequence Data; D010016:Osteoma / Q000382:microbiology*; D012091:Repetitive Sequences, Nucleic Acid; D014779:Virus Replication
D004279:DNA, Viral