title: Changes in pyridine and adenine nucleotide levels in Friend erythroleukaemia cells during growth and differentiation.
abstract: Pyridine and adenine nucleotide levels were measured in Friend erythroleukaemia cells (FELC) stimulated to growth and induced to differentiate by hexamethylene bisacetamide (HMBA) and N'-methylnicotinamide (N'-MNAM). A three- to fourfold increase in the NADP(H) was found to parallel cell growth stimulation in both the presence and absence of differentiation inducers. NAD(H) increased about twofold in control and to a minor extent in HMBA-treated FELC but did not vary significantly in N'-MNAM-treated cells. ATP was significantly higher in control cells stimulated to growth than in resting ones, but it did not vary in inducer-treated cells. These data confirm the relationship between high NADP(H) levels and cell resumption to growth; moreover they show that NAD(H) pool reduction and NAD/NADH ratio rise are associated with the process of FELC differentiation. The activities of NAD pyrophosphorylase and NAD kinase are much more enhanced in growth-stimulated FELC than in resting ones. On the other hand transition from the quiescent to the proliferative state was accompanied by a decrease in the activity of poly(ADP-ribose) polymerase. A decrease in poly(ADP-ribose) polymerase activity was also found in differentiated cells in contrast to controls.
authors.affiliation: Istituto di Patologia Generale, Università di Firenze, Italy.
authors.forename: R
authors.initials: R
authors.lastname: Caldini
authors.forename: M
authors.initials: M
authors.lastname: Chevanne
authors.forename: L
authors.initials: L
authors.lastname: Magnelli
mesh_terms: D000081:Acetamides / Q000494:pharmacology; D000227:Adenine Nucleotides / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D002455:Cell Division / Q000502:physiology; D005622:Friend murine leukemia virus / Q000302:isolation & purification*; D006397:Hematinics / Q000494:pharmacology; D004915:Leukemia, Erythroblastic, Acute / Q000378:metabolism* / Q000382:microbiology / Q000473:pathology*; D051379:Mice; D009243:NAD / Q000378:metabolism*; D009249:NADP / Q000378:metabolism*; D009536:Niacinamide / Q000031:analogs & derivatives / Q000494:pharmacology; D009612:Nicotinamide-Nucleotide Adenylyltransferase / Q000032:analysis / Q000378:metabolism; D010770:Phosphotransferases / Q000032:analysis / Q000378:metabolism; D017853:Phosphotransferases (Alcohol Group Acceptor); D011065:Poly(ADP-ribose) Polymerases / Q000032:analysis / Q000378:metabolism; D014407:Tumor Cells, Cultured / Q000737:chemistry / Q000378:metabolism / Q000473:pathology
chemical_list: D000081:Acetamides; D000227:Adenine Nucleotides; D006397:Hematinics; D009243:NAD; D009536:Niacinamide; D009249:NADP; D000255:Adenosine Triphosphate; D011065:Poly(ADP-ribose) Polymerases; D010770:Phosphotransferases; D017853:Phosphotransferases (Alcohol Group Acceptor); C021844:NAD kinase; D009612:Nicotinamide-Nucleotide Adenylyltransferase; C014026:hexamethylene bisacetamide; C008472:N-methylnicotinamide