[Suppression of myeloperoxidase gene expression by gamma-interferon].
The gene expression of myeloperoxidase (MPO) in HL60 cells, as tested by Northern blot analysis with a MPO gene probe, was markedly suppressed by treatment with gamma interferon (IFN-gamma)(200U/ml) for 9hr, whereas cytochemically detected MPO activity, cell surface antigen expression, and cell morphology remained unchanged even at 48hr after the treatment. IFN-gamma of 50U/ml was sufficient for the suppression at 24hr. When the HL60 cells treated with IFN-gamma (200U/ml) for 24hr were cultured in the absence of IFN-gamma for another 24hr, the transcript of MPO gene reverted to a level comparable to that of the HL60 cells cultured thoroughly in the absence of IFN-gamma, indicating the reversibility of the suppression. The suppression of MPO expression at RNA level, possibly independent of differentiation, is one of the biological activities exerted by IFN-gamma, which has not been previously reported.
Kawano
S
S
Department of Laboratory Medicine, Kobe University School of Medicine, Japan.
Tatsumi
E
E
Yoneda
N
N
Yamaguchi
N
N
D000199:Actins; D002455:Cell Division; D002478:Cells, Cultured; D003864:Depression, Chemical; D015971:Gene Expression Regulation, Enzymologic; D005805:Genes, MHC Class I / Q000235:genetics; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology*; D009195:Peroxidase / Q000235:genetics*; D012333:RNA, Messenger
D000199:Actins; D012333:RNA, Messenger; D007371:Interferon-gamma; D009195:Peroxidase