Stimulation of thymidine kinase activity in baculovirus infected cells is not due to a virus-coded enzyme.
A polyhedrin-positive recombinant autographa californica nuclear polyhedrosis virus (AcNPV) carrying a herpes simplex virus thymidine kinase gene under the control of the SynXIV promoter, a fusion of synthetic and linker-modified polyhedrin promoters, has been constructed. When this recombinant baculovirus was used to infect a variant of Spodoptera frugiperda cells deficient in thymidine kinase (TK-), a high level of TK activity was detected. These results, in conjunction with the demonstration that AcNPV could replicate in TK- S. frugiperda cells and no TK activity was found throughout infection, imply that the wild type virus-stimulated TK activity observed in S. frugiperda (TK+) cells is not contributed by a virus-coded enzyme.
Wang
X
X
Institute of Entomology, Zhongshan University, Guangzhou, People's Republic of China.
Xie
W
W
Long
Q
Q
He
D
D
Lin
G
G
Pang
Y
Y
Pu
Z
Z
D000818:Animals; D016367:Baculoviridae / Q000201:enzymology* / Q000235:genetics; D001483:Base Sequence; D002478:Cells, Cultured; D005814:Genes, Viral; D005822:Genetic Vectors; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D009036:Moths; D000077183:Occlusion Body Matrix Proteins; D011401:Promoter Regions, Genetic; D018139:Simplexvirus / Q000201:enzymology / Q000235:genetics; D013937:Thymidine Kinase / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics; D015678:Viral Structural Proteins / Q000235:genetics*; D014777:Virus Diseases / Q000201:enzymology*
D000077183:Occlusion Body Matrix Proteins; D014764:Viral Proteins; D015678:Viral Structural Proteins; C009829:polyhedrin protein, Nucleopolyhedrovirus; D013937:Thymidine Kinase