The use of antibodies labelled with dyes for fast and simple assay of some human proteins by immunofiltration technique.
Immunofiltration technique with polyclonal and monoclonal antibodies for semi-quantitative assays of human albumin, chorionic gonadotropin, immunoglobulin G and transferrin was elaborated. An amount of antibody was immobolized in the form of 6 radially located small bars on a dry test filter made of glass microfibre sheet. The other amount of antibody, used in solution, was labelled with some dyes like commercial disperse dyes, colloidal elements, formazans and polypyrrole. Number of colour bars appearing on the test filter showed ranged of analyte concentration. Good results were obtained using antibodies labelled with colloidal gold, Disperse Red 11 and formazan from MTT. Assays with monoclonal antibodies were more sensitive than with polyclonal antibodies.
Szewczuk
A
A
Biochemical Laboratory, Polish Academy of Sciences, Wrocław.
Kuropatwa
M
M
Rapak
A
A
D000906:Antibodies; D000911:Antibodies, Monoclonal; D006063:Chorionic Gonadotropin / Q000032:analysis; D004396:Coloring Agents; D005069:Evaluation Studies as Topic; D005374:Filtration / Q000379:methods; D017979:Gold Colloid; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods*; D007074:Immunoglobulin G / Q000032:analysis; D011506:Proteins / Q000032:analysis*; D012643:Selenium; D012709:Serum Albumin / Q000032:analysis; D014168:Transferrin / Q000032:analysis
D000906:Antibodies; D000911:Antibodies, Monoclonal; D006063:Chorionic Gonadotropin; D004396:Coloring Agents; D017979:Gold Colloid; D007074:Immunoglobulin G; D011506:Proteins; D012709:Serum Albumin; D014168:Transferrin; D012643:Selenium