Altered diacylglycerol level and metabolism in neutrophils from patients with localized juvenile periodontitis.
Diacylglycerol, a physiological activator of protein kinase C, was elevated nearly twofold in unstimulated peripheral blood neutrophils from patients with localized juvenile periodontitis compared with cells from normal individuals. These cells also showed an enhanced and prolonged elevation of diglyceride in response to N-formylmethionylleucylphenylalanine. The metabolism of a cell-permeant diacylglycerol by diglyceride kinase was significantly decreased, because of a fivefold or higher elevation in the apparent Km of cellular diglyceride kinase.
Tyagi
S R
SR
Department of Biochemistry, Emory University Medical School, O. Wayne Rollins Research Center, Atlanta, Georgia 30322.
Uhlinger
D J
DJ
Lambeth
J D
JD
Champagne
C
C
Van Dyke
T E
TE
D000328:Adult; D002118:Calcium / Q000378:metabolism; D019852:Diacylglycerol Kinase; D004075:Diglycerides / Q000032:analysis* / Q000378:metabolism; D006801:Humans; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000737:chemistry / Q000378:metabolism*; D010518:Periodontitis / Q000378:metabolism*; D010716:Phosphatidylinositols / Q000378:metabolism; D010770:Phosphotransferases / Q000032:analysis
D004075:Diglycerides; D010716:Phosphatidylinositols; D009240:N-Formylmethionine Leucyl-Phenylalanine; D010770:Phosphotransferases; D019852:Diacylglycerol Kinase; D002118:Calcium