title: Characterization of the mouse PDGF A-chain gene. Evolutionary conservation of gene structure, nucleotide sequence and alternative splicing.
abstract: The mouse platelet-derived growth factor (PDGF) A-chain gene has been structurally characterized and compared with its human counterpart. The organization of the two genes is similar. Both consist of 7 exons spaced by 6 introns of corresponding sizes. As in the human gene, exon 6 encodes a sequence which is alternatively spliced. When present, it codes for an alternative C-terminus of the A-chain. In intron 5, conserved stretches of nucleotides, potentially involved in the regulation of the alternative splicing, are identified. The untranslated sequences show a high degree of nucleotide sequence identity and several conserved consensus binding sites for transcription factors are identified within the 5' untranslated as well as in the 5' flanking region.
authors.affiliation: Department of Pathology, University Hospital, Uppsala, Sweden.
authors.forename: F
authors.initials: F
authors.lastname: Rorsman
authors.forename: C
authors.initials: C
authors.lastname: Betsholtz
mesh_terms: D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005075:Biological Evolution; D003001:Cloning, Molecular; D017124:Conserved Sequence; D005091:Exons; D015698:Genomic Library; D006801:Humans; D007438:Introns; D051379:Mice / Q000235:genetics*; D008969:Molecular Sequence Data; D010982:Platelet-Derived Growth Factor / Q000096:biosynthesis / Q000235:genetics*; D011401:Promoter Regions, Genetic; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D016385:TATA Box
chemical_list: D010982:Platelet-Derived Growth Factor; C075278:platelet-derived growth factor A