title: Purification and crystallization of insecticidal delta-endotoxin CryIIIB2 from Bacillus thuringiensis.
abstract: CryIIIB2, an insecticidal protein from Bacillus thuringiensis has been crystallized from 0.6 M NaBr and HEPES buffer at pH 7.0 and X-ray diffraction data collected on a native crystal to 2.4 A. The insecticidal protein was obtained from a Bacillus thuringiensis (Bt) strain EG7231. Crystals of the endotoxin are orthorhombic, space group C2221, with unit cell dimensions of a = 122.44, b = 131.81, and c = 105.37 A. A unit cell contains one molecule of the 67,000 Da endotoxin per asymmetric unit.
authors.affiliation: Molecular Biophysics Department, Medical Foundation of Buffalo, New York 14203.
authors.forename: V
authors.initials: V
authors.lastname: Cody
authors.forename: J R
authors.initials: JR
authors.lastname: Luft
authors.forename: E
authors.initials: E
authors.lastname: Jensen
authors.forename: W
authors.initials: W
authors.lastname: Pangborn
authors.forename: L
authors.initials: L
authors.lastname: English
mesh_terms: D001413:Bacillus thuringiensis / Q000737:chemistry*; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D003460:Crystallization; D004731:Endotoxins / Q000737:chemistry / Q000302:isolation & purification*; D006460:Hemolysin Proteins; D007306:Insecticides / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004731:Endotoxins; D006460:Hemolysin Proteins; D007306:Insecticides; C030956:insecticidal crystal protein, Bacillus Thuringiensis