A novel terminal oxidase, cytochrome baa3 purified from aerobically grown Pseudomonas aeruginosa: it shows a clear difference between resting state and pulsed state.
A novel type of cytochrome c oxidase was purified to homogeneity from Pseudomonas aeruginosa which was grown aerobically. The purified oxidase contained two molecules of heme a, two atoms of copper, and one molecule of protoheme per molecule. One of the two heme a molecules in the oxidase reacted with carbon monoxide, so that the enzyme was of baa3-type. The oxidase molecule was composed of three subunits with molecular weights of 38,000, 57,000, and 82,000. Although the oxidase oxidized ferrocytochrome c-550 obtained from the bacterial cells grown aerobically, the oxidizing activity was not high. The "resting form" and the "pulsed form" of the oxidase were observed clearly with this enzyme, and the transition from the resting form to the pulsed form was accompanied by a distinct change of the enzymatic activity. The difference in the kinetics of the catalytic reactions between the two forms is discussed.
Fujiwara
T
T
Department of Life Science, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Kanagawa.
Fukumori
Y
Y
Yamanaka
T
T
D002248:Carbon Monoxide / Q000378:metabolism; D002384:Catalysis; D002850:Chromatography, Gel; D003300:Copper / Q000032:analysis / Q000378:metabolism; D003574:Cytochrome c Group / Q000378:metabolism; D003576:Electron Transport Complex IV / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D006418:Heme / Q000032:analysis / Q000378:metabolism; D008970:Molecular Weight; D010084:Oxidation-Reduction; D011550:Pseudomonas aeruginosa / Q000201:enzymology* / Q000254:growth & development; D013056:Spectrophotometry, Ultraviolet; D013772:Tetramethylphenylenediamine / Q000378:metabolism
D003574:Cytochrome c Group; D006418:Heme; D003300:Copper; D002248:Carbon Monoxide; D003576:Electron Transport Complex IV; D013772:Tetramethylphenylenediamine