The human fibroblast receptor for gp86 of human cytomegalovirus is a phosphorylated glycoprotein.
A human embryonic lung (HEL) cell receptor for gp86 of human cytomegalovirus that functions in virus-cell fusion was further characterized. Anti-idiotype antibodies that mimic gp86 were used to immunoprecipitate the 92.5-kDa fibroblast membrane receptor for gp86, which was preincubated with various endoglycosidases. The receptor, which has a pI ranging from 5.3 to 5.6, appears to be a glycoprotein with primarily N-linked sugar residues, some of which have high concentrations of mannose and some of which are complex oligosaccharides. Western blots (immunoblots) of electrophoretically transferred receptor incubated with various biotinylated lectins confirmed the presence of sugar moieties, including N-acetylglucosamine, glucose or mannose, and galactose, but not fucose or N-acetylgalactosamine. This gp86 receptor from uninfected HEL cells also incorporated radiolabeled phosphate from orthophosphoric acid, indicating that it is a constitutively phosphorylated receptor.
Keay
S
S
Research Service, Department of Veterans Affairs Medical Center, Baltimore, Maryland.
Baldwin
B
B
D000818:Animals; D000888:Antibodies, Anti-Idiotypic; D015153:Blotting, Western; D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000032:analysis; D002460:Cell Line; D003587:Cytomegalovirus / Q000502:physiology*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D017874:Immediate-Early Proteins / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000096:biosynthesis / Q000302:isolation & purification / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010710:Phosphates / Q000378:metabolism; D010750:Phosphoproteins / Q000096:biosynthesis / Q000302:isolation & purification / Q000502:physiology*; D011991:Receptors, Virus / Q000096:biosynthesis / Q000302:isolation & purification / Q000502:physiology*; D015534:Trans-Activators; D014759:Viral Envelope Proteins / Q000378:metabolism*; D014764:Viral Proteins
D000888:Antibodies, Anti-Idiotypic; D002241:Carbohydrates; C069186:IE2 protein, Cytomegalovirus; D017874:Immediate-Early Proteins; D008562:Membrane Glycoproteins; D010710:Phosphates; D010750:Phosphoproteins; D011991:Receptors, Virus; D015534:Trans-Activators; C077406:UL115 protein, Human herpesvirus 5; D014759:Viral Envelope Proteins; D014764:Viral Proteins; C071440:glycoprotein H, Cytomegalovirus; C499358:glycoprotein H, Human cytomegalovirus; C439068:glycoprotein O, cytomegalovirus