Transposon mutagenesis in Legionella pneumophila. I.--Persistence of suicide and broad host-range plasmids.
Two of three highly virulent strains of Legionella pneumophila could act as recipients at high frequencies in conjugation experiments with Escherichia coli donor strains carrying broad host-range plasmids belonging to incompatibility groups N, P and W. All broad host-range and most transposon-delivery plasmids persisted within transconjugants with high stability. Only one (pSUP1021) of several vehicles designed for the delivery of transposons into the chromosome of Gram-negative bacteria was found to yield transposon mutants of Legionella at a detectable frequency.
Tully
M
M
Division of Pathology, PHLS Centre for Applied Microbiology and Research, Porton Down, Salisbury, UK.
Sharpe
S A
SA
Ashworth
S
S
D015139:Blotting, Southern; D003227:Conjugation, Genetic / Q000502:physiology*; D004261:DNA Replication; D004251:DNA Transposable Elements / Q000502:physiology*; D004926:Escherichia coli / Q000235:genetics; D066298:In Vitro Techniques; D016952:Legionella pneumophila / Q000235:genetics*; D016254:Mutagenesis, Insertional; D010957:Plasmids / Q000235:genetics*
D004251:DNA Transposable Elements