Fusion of a scid pre-B cells with a wild type (myeloma) B cell results in correct rearrangement of a V(D)J recombination substrate.
Mice with the scid mutation have a defect in the V(D)J recombinase. In order to determine whether the SCID product is normally present in mature B cells that do not have the recombinase activity, scid pre-B cells were fused with myeloma cells. It was found that in the hybrid cells, a rearrangement test gene was correctly joined immediately after fusion. The same test gene was aberrantly rearranged in the scid pre-B cells. Stable hybrids between the scid pre-B and the myeloma cells had lost the expression of RAG-1 and RAG-2 genes, supporting the previous finding of an inhibitor of rearrangement in myeloma cells that acts shortly after fusion. Thus, mature B cells apparently contain the SCID product, the wild type SCID function is not competitively interfered with by products present in scid pre-B cells, and the SCID product seems not to be a target for the recombinase inhibitor.
Zhao
J
J
Department of Molecular Genetics and Cell Biology, University of Chicago, IL 60637.
Storb
U
U
D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002459:Cell Fusion / Q000276:immunology*; D015322:Gene Rearrangement, B-Lymphocyte / Q000502:physiology*; D005803:Genes, Immunoglobulin / Q000502:physiology*; D051379:Mice; D016513:Mice, SCID; D008969:Molecular Sequence Data; D009101:Multiple Myeloma / Q000276:immunology; D016133:Polymerase Chain Reaction; D014407:Tumor Cells, Cultured