Characterization of primary human fibroblasts transformed by human papilloma virus type 16 and herpes simplex virus type 2 DNA sequences.
Human papilloma virus type 16 (HPV-16) and herpes simplex virus type 2 (HSV-2) are human viruses implicated in the development of cancer, in particular cervical cancer. The ability of HSV-2 and HPV-16 to transform early passage human cells was examined in this report. For these studies, gingival fibroblasts were utilized. One gingival cell strain was derived from a normal individual (N-16). The second cell strain was derived from hyperplastic gingival tissue of an epileptic individual (R-30) treated with phenytoin, an antiseizure drug. A common side effect of phenytoin is the induction of gingival overgrowth. R-30 cells contained a stable chromosomal translocation between chromosomes 8 and 18 and expressed higher steady state levels of c-myc. HPV-16 DNA efficiently immortalized R-30 cells but not N-16 cells. R-30 cells cotransfected with HPV-16, and HSV-2 viral DNAs were more aneuploid than R-30 cells transfected with HPV-16 DNA alone. Additionally, R-30 cells cotransfected with both viral DNAs grew better in soft agar than R-30 cells transfected with HPV-16 DNA alone. HSV-2 DNA was detected in transformed cells by polymerase chain reaction. These results suggested R-30 cells were immortalized more efficiently by HPV-16 and further imply that HPV-16 and HSV-2 DNA fragments can cooperate during multistep transformation.
Dhanwada
K R
KR
Department of Microbiology, Loyola University of Chicago Medical Center, Maywood, Illinois 60153.
Veerisetty
V
V
Zhu
F
F
Razzaque
A
A
Thompson
K D
KD
Jones
C
C
D001483:Base Sequence; D002455:Cell Division; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D004279:DNA, Viral / Q000235:genetics*; D005347:Fibroblasts / Q000166:cytology / Q000382:microbiology; D005881:Gingiva / Q000382:microbiology; D006801:Humans; D007621:Karyotyping; D008969:Molecular Sequence Data; D027383:Papillomaviridae / Q000235:genetics*; D018139:Simplexvirus / Q000235:genetics*; D014412:Tumor Virus Infections / Q000235:genetics
D004279:DNA, Viral