title: Analysis of heterologous and homologous promoters and enhancers in vitro and in vivo by gene transfer into Japanese medaka (Oryzias latipes) Xiphophorus.
abstract: Efficient expression systems are required for analysis of gene regulation and function in teleost fish. To develop such systems, a number of inducible or constitutive promoter and enhancer sequences of fish or higher vertebrate origin were tested for activity in a variety of fish cell lines and in embryos of the Japanese medaka fish (Oryzias latipes) and Xiphophorus. The activity of the different promoter-enhancer combinations were quantitated. Considerable differences were found for some constructs if tested in vitro or in vivo. Fro the data obtained, a set of expression vectors for basic research as well as for aquaculture purposes were established.
authors.affiliation: Max-Planck-Institüt für Biochemie, Martinsried, Germany.
authors.forename: C
authors.initials: C
authors.lastname: Winkler
authors.forename: Y
authors.initials: Y
authors.lastname: Hong
authors.forename: J
authors.initials: J
authors.lastname: Wittbrodt
authors.forename: M
authors.initials: M
authors.lastname: Schartl
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified / Q000235:genetics*; D015139:Blotting, Southern; D006528:Carcinoma, Hepatocellular; D015500:Chloramphenicol O-Acetyltransferase / Q000096:biosynthesis / Q000235:genetics; D004247:DNA / Q000737:chemistry; D004742:Enhancer Elements, Genetic; D005399:Fishes / Q000196:embryology / Q000235:genetics*; D005786:Gene Expression Regulation; D005822:Genetic Vectors; D006801:Humans; D008113:Liver Neoplasms; D008668:Metallothionein / Q000235:genetics; D008845:Microinjections; D009690:Nucleic Acid Conformation; D009990:Oryzias / Q000196:embryology / Q000235:genetics; D011401:Promoter Regions, Genetic; D013937:Thymidine Kinase / Q000235:genetics; D014162:Transfection / Q000379:methods*; D014337:Trout / Q000235:genetics; D014407:Tumor Cells, Cultured
chemical_list: D004247:DNA; D008668:Metallothionein; D015500:Chloramphenicol O-Acetyltransferase; D013937:Thymidine Kinase