Use of ameroid constrictors in the development of renin-dependent hypertension in dogs.
We describe methods for using ameroid constrictors to develop renin-dependent hypertension in dogs. A vascular-access-port was implanted in each dog and mean arterial blood pressure was monitored. Via a small flank incision, an ameroid constrictor was placed on the left renal artery and mean arterial blood pressure, plasma renin activity, and the response to angiotensin-converting enzyme inhibition measured during the subsequent 4 weeks. Placement of the constrictor on the left renal artery resulted in a significant increase in plasma renin activity which was accompanied by a significant hypertension (blood pressure greater than 140 mmHg). The greatest increase in plasma renin activity and blood pressure occurred at week two which coincided with the greatest response to angiotensin-converting enzyme inhibition, when mean arterial blood pressure decreased more than 30 mmHg after administration of either captopril or enalapril (1 mg/kg, i.v.). The results of this study indicate that ameroid constrictors provide a reliable method for establishing renin-dependent hypertension in dogs.
Brooks
D P
DP
Department of Pharmacology, SmithKline Beecham Pharmaceuticals, King of Prussia, PA 19406.
Fredrickson
T A
TA
D000818:Animals; D001672:Biocompatible Materials / Q000009:adverse effects; D001794:Blood Pressure / Q000187:drug effects; D002364:Caseins; D003250:Constriction; D004195:Disease Models, Animal; D004285:Dogs; D006339:Heart Rate / Q000187:drug effects; D020100:Hydrogels; D006977:Hypertension, Renal / Q000097:blood / Q000209:etiology*; D008297:Male; D012077:Renal Artery / Q000601:surgery; D012083:Renin / Q000097:blood / Q000187:drug effects / Q000502:physiology*
D001672:Biocompatible Materials; D002364:Caseins; D020100:Hydrogels; C009719:ameroid; D012083:Renin