Dietary n-3 fatty acids reduce antibody-dependent cell cytotoxicity and alter eicosanoid release by chicken immune cells.
The overall goal of the present study was to determine whether the incorporation of n-3 fatty acids into poultry rations would alter the immune response of broiler chickens. Female broiler chicks were fed a corn and soybean meal-based diet to which one of four dietary fats were added: lard (LA), corn oil (CO), flaxseed oil (SO), or menhaden fish oil (FO). The latter two fat sources are rich in n-3 polyunsaturated fatty acids (PUFA). Enriching the diet with n-3 PUFA did not alter the primary or secondary antibody response of broiler chickens to sheep red blood cells. Dietary fat source had no effect on antibody-dependent cell cytotoxicity (ADCC) by peripheral blood leukocytes, but ADCC by splenocytes was 50% lower in chickens fed SO and FO compared with LA and CO (P less than .005). As expected, the fatty acid profile of the isolated immune cells reflected the fatty acid composition of the dietary fats fed. Basal release and calcium ionophore (A23187)-stimulated (10 microM) release of thromboxane B were significantly lower (P less than .05) in the SO and FO groups compared with the LA and CO groups. Total leukotriene B release was not significantly altered by dietary fat source. In conclusion, feeding broiler chickens diets rich in n-3 PUFA reduced ADCC of splenocytes and altered eicosanoid production by isolated immune cells.
Fritsche
K L
KL
Department of Animal Sciences, University of Missouri-Columbia 65211.
Cassity
N A
NA
D000818:Animals; D000917:Antibody Formation; D000920:Antibody-Dependent Cell Cytotoxicity / Q000187:drug effects*; D000001:Calcimycin / Q000494:pharmacology; D002645:Chickens / Q000276:immunology*; D004041:Dietary Fats / Q000494:pharmacology*; D015777:Eicosanoids / Q000378:metabolism*; D005227:Fatty Acids / Q000378:metabolism; D015525:Fatty Acids, Omega-3 / Q000494:pharmacology*; D005260:Female; D005395:Fish Oils / Q000494:pharmacology; D007962:Leukocytes / Q000276:immunology / Q000378:metabolism; D007975:Leukotriene B4 / Q000378:metabolism; D013154:Spleen / Q000166:cytology / Q000276:immunology; D013929:Thromboxane B2 / Q000378:metabolism
D004041:Dietary Fats; D015777:Eicosanoids; D005227:Fatty Acids; D015525:Fatty Acids, Omega-3; D005395:Fish Oils; C033266:Menhaden oil; D007975:Leukotriene B4; D000001:Calcimycin; D013929:Thromboxane B2