Tissue-print hybridization using a non-radioactive probe for the detection of infectious bursal disease virus.
Tissue-print hybridization was evaluated as a simplified means for detection of infectious bursal disease virus (IBDV) in the bursa of Fabricius from infected chickens. The assay employed a biotin-labeled synthetic oligonucleotide as a probe. The bound probe was detected using a color assay consisting of streptavidin conjugated to alkaline phosphatase. Bursae were imprinted onto nitrocellulose and then hybridized with the biotinylated probe. Bursal prints from IBDV-infected chickens were readily distinguished from control prints by color development and differences in signal intensity.
Hathcock
T L
TL
Department of Pathobiology, College of Veterinary Medicine, Auburn University, Alabama 36849-5519.
Giambrone
J J
JJ
D000818:Animals; D001483:Base Sequence; D002060:Bursa of Fabricius / Q000382:microbiology*; D002645:Chickens; D007243:Infectious bursal disease virus / Q000235:genetics / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes / Q000737:chemistry; D011201:Poultry Diseases / Q000175:diagnosis / Q000382:microbiology*; D012367:RNA, Viral / Q000032:analysis; D012088:Reoviridae Infections / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D013047:Specific Pathogen-Free Organisms
D015345:Oligonucleotide Probes; D012367:RNA, Viral