title: [Interclonal and interpopulational differences in the frequency of spontaneous karyotypic changes in cell populations of transplantable rat rhabdomyosarcoma RA-2].
abstract: Rat organospecific transplantable RA-2 rhabdomyosarcoma substrains RA-2H (high metastatic potential), RA-2L (low metastatic potential) and RA-2T (thermal resistance) were investigated using single cell cloning technique for frequency of micronucleated cells (FMC). Significant interclonal and interpopulational differences were established. Average FMC for RA-2H was 2.96 +/- 0.13%; 5.94 +/- 0.24% for RA-2L and 1.89 +/- 0.12% for RA-2T. Clones showed 5-10 times differences in FMC in each substrain. FMC versus growth time and clone size was studied in RA-2H: average FMC dropped from 2.7% on day 9 to 0.5% on day 20 after administration and the lowest values were recorded in larger clones at each stage. Perspectives of FMC studies in tumor cell populations are discussed.
authors.forename: V Iu
authors.initials: VIu
authors.lastname: Kravtsov
authors.forename: E V
authors.initials: EV
authors.lastname: Fedorova
authors.forename: E V
authors.initials: EV
authors.lastname: Kaminskaia
authors.forename: I V
authors.initials: IV
authors.lastname: Guzhova
authors.forename: A F
authors.initials: AF
authors.lastname: Iakovlev
authors.forename: Iu B
authors.initials: IuB
authors.lastname: Vakhtin
mesh_terms: D000818:Animals; D002452:Cell Count; D007621:Karyotyping; D008168:Lung / Q000648:ultrastructure; D008175:Lung Neoplasms / Q000648:ultrastructure*; D048629:Micronuclei, Chromosome-Defective / Q000648:ultrastructure; D009368:Neoplasm Transplantation; D009928:Organ Specificity; D051381:Rats; D012208:Rhabdomyosarcoma / Q000648:ultrastructure*; D013997:Time Factors; D014407:Tumor Cells, Cultured / Q000648:ultrastructure
vernacular_title: Mezhklonal'nye i mezhpopuliatsionnye razlichiia po chastote spontannykh kariotipicheskikh izmeneniĭ v kletochnykh populiatsiiakh perevivnoĭ rabdomiosarkomy RA-2 krys.