title: Differences in macrophage stimulation and leukocyte accumulation in response to intraperitoneal administration of glucose/mannose-binding plant lectins.
abstract: Peritoneal macrophage stimulation (rapid spreading on glass surface and hydrogen peroxide production) and inflammatory reaction (leukocyte accumulation) obtained in C3H/HeJ mice at 8 weeks of age, after a single ip injection of 10 micrograms concanavalin A (Con A), a lectin extracted from Canavalia ensiformis, were compared with those obtained with two other glucose/mannose-binding lectins extracted from Canavalia brasiliensis (Con Br) and Dioclea grandiflora (DGL). All lectins enhanced macrophage spreading 3- to 4-fold at 24-72 h compared to control. Stimulation of hydrogen peroxide release by Con A, Con Br and DGL lasted 1, 2 and 3 days, respectively. Leukocyte cell influx at 24-72 h after lectin injection consisted mainly of mononuclear cells. Con A induced a moderate increase in the total number of peritoneal cells, whereas administration of Con Br or DGL increased the number of peritoneal cells 2- to 3-fold. The results indicate that DGL and Con Br have more pronounced effects on macrophage stimulation and inflammatory reactions than Con A.
authors.affiliation: Departamento de Imunologia, Universidade de São Paulo, Brasil.
authors.forename: D
authors.initials: D
authors.lastname: Rodriguez
authors.forename: B S
authors.initials: BS
authors.lastname: Cavada
authors.forename: J T
authors.initials: JT
authors.lastname: Abreu-de-Oliveira
authors.forename: R
authors.initials: R
authors.lastname: de-Azevedo-Moreira
authors.forename: M
authors.initials: M
authors.lastname: Russo
mesh_terms: D000818:Animals; D005947:Glucose / Q000378:metabolism*; D007274:Injections, Intraperitoneal; D037102:Lectins / Q000008:administration & dosage* / Q000494:pharmacology; D007962:Leukocytes / Q000187:drug effects* / Q000276:immunology; D008262:Macrophage Activation / Q000187:drug effects* / Q000276:immunology; D017737:Macrophages, Peritoneal / Q000187:drug effects* / Q000276:immunology; D008358:Mannose / Q000378:metabolism*; D051379:Mice; D008809:Mice, Inbred C3H; D013268:Stimulation, Chemical; D013997:Time Factors
chemical_list: D037102:Lectins; D005947:Glucose; D008358:Mannose