title: Captopril does not protect against renal oxidative injury.
abstract: The free thiol group of captopril is important in the action and metabolism of this drug. It has been postulated that the thiol group may allow captopril to act in a manner similar to glutathione and protect against oxidative injury. This study investigated the ability of captopril, enalaprilat, and N-acetylcysteine to prevent tertbutyl hydroperoxide induced oxidative injury in rat renal homogenates. Lipid peroxidation was significantly increased in homogenates from captopril-treated animals (p < 0.05), and following glutathione depletion this was further enhanced (p < 0.001). Renal glutathione content was significantly reduced by captopril treatment (p < 0.01). These results suggest that captopril does not act as an alternate source of reducing equivalents to glutathione and does not protect against renal oxidative injury in this model.
authors.affiliation: School of Pharmacy, University of Otago, Dunedin, New Zealand.
authors.forename: S A
authors.initials: SA
authors.lastname: Chin
authors.forename: J P
authors.initials: JP
authors.lastname: Fawcett
authors.forename: R J
authors.initials: RJ
authors.lastname: Walker
mesh_terms: D000111:Acetylcysteine / Q000494:pharmacology; D000818:Animals; D002216:Captopril / Q000493:pharmacokinetics / Q000494:pharmacology*; D004347:Drug Interactions; D015773:Enalaprilat / Q000494:pharmacology; D005978:Glutathione / Q000378:metabolism; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D010084:Oxidation-Reduction / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D013438:Sulfhydryl Compounds / Q000493:pharmacokinetics; D013997:Time Factors
chemical_list: D013438:Sulfhydryl Compounds; D002216:Captopril; D005978:Glutathione; D015773:Enalaprilat; D000111:Acetylcysteine