Deoxyribonucleolytic activity of alpha- and beta-momorcharins.
alpha- and beta-Momorcharins were purified by an improved procedure using the affinity Affi-gel Blue gel and the ion exchange Mono-S FPLC column. Both purified alpha- and beta-momorcharins possessed deoxyribonucleolytic activity. Under normal digestion conditions, they cleaved the supercoiled, double-stranded SV-40 DNA to produce nicked circular and linear DNAs. Prolonged incubation did not have any further effects. On the other hand, the linear DNAs, lambda, Ad-2 and T7 were not digested by alpha- nor beta-momorcharin. Thus, it appears that the conformation of the DNA may be the determining factor for the deoxyribonucleolytic activity of these momorcharins.
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Department of Biochemistry, Chinese University of Hong Kong, Shatin, N.T.
Yeung
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Fong
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D002850:Chromatography, Gel; D004279:DNA, Viral / Q000187:drug effects*; D004591:Electrophoresis, Polyacrylamide Gel; D010940:Plant Proteins / Q000302:isolation & purification / Q000494:pharmacology*; D012269:Ribosomal Proteins; D054788:Ribosome Inactivating Proteins; D013539:Simian virus 40 / Q000235:genetics
D004279:DNA, Viral; D010940:Plant Proteins; D012269:Ribosomal Proteins; C039582:MMC protein, Momordica charantia; D054788:Ribosome Inactivating Proteins