Balancing of energy-consuming processes of K 562 cells.
A balance of ATP-consuming processes in human erythroleukemia (K 562) cells by use of the decreased 14CO2 formation from [1-14C]-glutamate following inhibition of energy-requiring processes is presented. This method was tested on Ehrlich mouse ascites tumour cells and was used in suspensions of K 562 cells with a low cell content. More than 90 percent of the ATP produced by oxidative phosphorylation could be accounted for in K 562 cells. Protein synthesis consumed about 35 percent, Na+/K(+)-ATPase about 20 percent and transcription processes 5-10 percent of the total ATP. The share of the Ca(2+)-dependent reactions was notably high at 25 percent in comparison with Ehrlich mouse ascites tumour cells, reticulocytes or hepatocytes. ATP consumption by DNA synthesis was assessed at 5-10 percent. Only less than 10 percent of the consumption of ATP produced oxidatively remained for other cellular reactions. The degree of coupling of K 562 cells was high in comparison with that of other eukaryotic cell types.
Siems
W G
WG
Institute of Biochemistry, Medical Faculty (Charité), Humboldt University, Berlin, Federal Republic of Germany.
Schmidt
H
H
Gruner
S
S
Jakstadt
M
M
D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000378:metabolism; D005260:Female; D005622:Friend murine leukemia virus; D004915:Leukemia, Erythroblastic, Acute / Q000378:metabolism*; D051379:Mice; D009374:Neoplasms, Experimental / Q000378:metabolism; D010085:Oxidative Phosphorylation; D014176:Protein Biosynthesis; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured
D000255:Adenosine Triphosphate; D000254:Sodium-Potassium-Exchanging ATPase