Characterization of experimental rat nephroblastoma and its cell line.
Rat nephroblastoma (Wilms' tumor) was induced by transplacental administration of N-ethyl-nitrosourea (ENU). The induced renal tumors were histologically compatible with human nephroblastoma. A cultured cell line (ENU-T-1) established from a xenotransplant, showed similar morphological and biological features to cultured embryonal kidney cells. Introduction of normal human chromosome #11 (#11) bearing Wilms' tumor suppressor gene(s) (WT) suppressed colony-forming ability on soft agar plates (CFA) but tumorigenicity of ENU-T-1 was not affected. Whereas tumorigenicity of human nephroblastoma cell line, SK-NEP-1 was completely suppressed, CFA was unchanged. These facts indicated that pathogenetic mechanism is different between human and experimental rat nephroblastomas.
Nagashima
Y
Y
Department of Pathology, Yokohama City University School of Medicine, Japan.
Miyagi
Y
Y
Sumino
K
K
Ohaki
Y
Y
Umeda
M
M
Oshimura
M
M
Misugi
K
K
D000818:Animals; D005038:Ethylnitrosourea; D006801:Humans; D007680:Kidney Neoplasms / Q000139:chemically induced / Q000473:pathology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014407:Tumor Cells, Cultured; D009396:Wilms Tumor / Q000139:chemically induced / Q000473:pathology*
D005038:Ethylnitrosourea