Identification of multiple biological factors in rabbit serum that modulate dopamine-mediated aortic constriction.
A bioassay was established using freshly prepared rabbit aortic strip to monitor the effects of various biological factors contained in rabbit serum that modulate vasoconstriction. Serotonin was shown to be a major vasoconstriction modulator in rabbit serum. A rapid procedure is described for its isolation from rabbit serum by sequential gel filtration chromatography and reversed-phase HPLC using C4, C18, and C2/C18 columns with an overall cumulative yield of about 20%. It was also noted that rabbit serum contains multiple biological factors that modulate dopamine-mediated aortic contraction other than serotonin. A potent vasoconstriction inhibitor was identified in rabbit serum which appears to be a novel regulator that mediates its effect via both alpha-adrenergic and serotonin receptors.
Leone
M G
MG
Population Council, New York, New York 10021.
Silvestrini
B
B
Palmery
M
M
Cheng
C Y
CY
D000818:Animals; D001013:Aorta, Thoracic / Q000502:physiology*; D001685:Biological Factors / Q000097:blood*; D004298:Dopamine / Q000502:physiology; D066298:In Vitro Techniques; D011817:Rabbits; D011942:Receptors, Adrenergic, alpha / Q000502:physiology; D011985:Receptors, Serotonin / Q000502:physiology; D012701:Serotonin / Q000097:blood / Q000502:physiology; D014661:Vasoconstriction / Q000502:physiology*
D001685:Biological Factors; D011942:Receptors, Adrenergic, alpha; D011985:Receptors, Serotonin; D012701:Serotonin; D004298:Dopamine