Isolation of amine oxidase from bovine plasma by a two-step procedure.
A novel method for isolation of amine oxidase from bovine plasma is reported; it involves a two-step procedure, namely ammonium sulfate fractionation and affinity chromatography with elution by aniline, which is a competitive inhibitor of the enzyme. A homogeneous enzyme, characterized by a specific activity of 0.44 U/mg, was obtained with a yield higher than 50%.
Vianello
F
F
Department of Biological Chemistry, University of Padova, Italy.
Di Paolo
M L
ML
Zennaro
L
L
Stevanato
R
R
Rigo
A
A
D006631:Amine Oxidase (Copper-Containing); D000588:Amines; D000818:Animals; D002417:Cattle; D002846:Chromatography, Affinity; D004578:Electron Spin Resonance Spectroscopy; D004591:Electrophoresis, Polyacrylamide Gel; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000037:antagonists & inhibitors / Q000097:blood* / Q000302:isolation & purification*; D012685:Sepharose / Q000031:analogs & derivatives
D000588:Amines; C024903:aminohexyl-sepharose; D012685:Sepharose; D006631:Amine Oxidase (Copper-Containing); D000587:Oxidoreductases Acting on CH-NH Group Donors