title: The effects of arachidonic acid and its CoA ester on the catabolism of prostaglandin E2 in rabbit kidney cortex.
abstract: The effects of arachidonic acid and arachidonoyl CoA on the catabolism of prostaglandin E2 in a 105000 x g supernatant fraction from rabbit kidney cortex were examined. Arachidonic acid reduced the 15-hydroxy prostaglandin dehydrogenase activity by 50% at 20 microM, while arachidonoyl CoA showed weak inhibition for the enzyme activity (15% at 20 microM). However, arachidonoyl CoA strongly inhibited the prostaglandin delta 13 reductase activity, the concentration required for 50% inhibition being about 3 microM. The dehydrogenase inhibition by arachidonic acid was non-competitive with regard to NAD+ and prostaglandin E2, respectively. Arachidonoyl CoA was also a non-competitive inhibitor for the reductase with regard to NADH and 15-keto prostaglandin E2, respectively. These results suggest that arachidonic acid and arachidonoyl CoA can be important modulating factors in prostaglandin catabolism by the kidney cortex.
authors.affiliation: Department of Hygienic Chemistry, Osaka University of Pharmaceutical Sciences, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Sakuma
authors.forename: Y
authors.initials: Y
authors.lastname: Fujimoto
authors.forename: M
authors.initials: M
authors.lastname: Okumura
authors.forename: H
authors.initials: H
authors.lastname: Nishida
authors.forename: I
authors.initials: I
authors.lastname: Yamamoto
authors.forename: T
authors.initials: T
authors.lastname: Fujita
mesh_terms: D011452:15-Oxoprostaglandin 13-Reductase / Q000037:antagonists & inhibitors; D000214:Acyl Coenzyme A / Q000494:pharmacology*; D000818:Animals; D016718:Arachidonic Acid / Q000494:pharmacology*; D015232:Dinoprostone / Q000031:analogs & derivatives / Q000378:metabolism*; D006911:Hydroxyprostaglandin Dehydrogenases / Q000037:antagonists & inhibitors; D066298:In Vitro Techniques; D007672:Kidney Cortex / Q000378:metabolism*; D008297:Male; D011817:Rabbits
chemical_list: D000214:Acyl Coenzyme A; C026563:arachidonyl-coenzyme A; D016718:Arachidonic Acid; C026346:15-ketoprostaglandin E2; D006911:Hydroxyprostaglandin Dehydrogenases; C035859:15-hydroxyprostaglandin dehydrogenase; D011452:15-Oxoprostaglandin 13-Reductase; D015232:Dinoprostone