Flow cytometric analysis of effects of cytokines on the expression of varicella-zoster virus glycoproteins.
Varicella-zoster virus (VZV)-infected human embryonic fibroblast (HEF) cells were stained with monoclonal antibodies directed against VZV glycoprotein I, II and IV, and then labeled with fluorescein isothiocyanate (FITC)-conjugated goat anti-mouse IgG. The cells were analyzed by flow cytometry. VZV-infected cells expressing VZV glycoproteins were clearly distinguished from uninfected cells. This method was useful for analyzing expression of VZV glycoproteins in different experimental conditions. Interferon alpha, beta, and gamma and tumor necrosis factor (TNF)-alpha reduced the percentage of positive cells and the mean fluorescence intensity of the cells expressing VZV glycoproteins. Interleukin(IL)-1 beta, IL-6 and TNF-beta had little effect on the expression of VZV glycoproteins.
Ito
M
M
Department of Pediatrics, Mie University School of Medicine, Japan.
Mizutani
K
K
Nakano
T
T
Yasuda
N
N
Kamiya
T
T
Kitamura
K
K
Torigoe
S
S
Ihara
T
T
Kamiya
H
H
Sakurai
M
M
D002478:Cells, Cultured; D002644:Chickenpox / Q000378:metabolism*; D016207:Cytokines / Q000494:pharmacology*; D005347:Fibroblasts; D005434:Flow Cytometry; D014645:Herpesvirus 3, Human / Q000378:metabolism*; D006801:Humans; D014760:Viral Fusion Proteins / Q000032:analysis* / Q000187:drug effects
D016207:Cytokines; D014760:Viral Fusion Proteins