Expression of platelet activating factor receptor in renal tubular cell line (LLC-PK1).
Northern blot analyses of poly(A)+ RNA extracted from cloned renal tubule cells, LLC-PK1, MDCK and JTC-12 cells, were performed using an isolated cDNA of guinea pig platelet activating factor (PAF) receptor as a probe. The results showed the expression of single entity of PAF receptor mRNA in LLC-PK1, but not in MDCK or JTC-12 cells. PAF also induced a dose-dependent elevation of intracellular calcium in LLC-PK1 cells as assessed by Fura-2 fluorescence signals, which was completely blocked by a PAF receptor antagonist, WEB 2170. These results indicate a PAF receptor-effector system in a renal tubular cell line LLC-PK1, providing a possible model system for studying physiological roles of PAF receptors in renal tubules.
Jamil
K M
KM
First Department of Internal Medicine, University of Tokyo, Japan.
Takano
T
T
Nakao
A
A
Honda
Z
Z
Shimizu
T
T
Watanabe
T
T
Kurokawa
K
K
D000818:Animals; D015152:Blotting, Northern; D002118:Calcium / Q000378:metabolism; D002460:Cell Line; D003001:Cloning, Molecular; D015342:DNA Probes; D004285:Dogs; D015870:Gene Expression; D006168:Guinea Pigs; D007684:Kidney Tubules / Q000378:metabolism*; D051379:Mice; D010972:Platelet Activating Factor / Q000494:pharmacology; D010980:Platelet Membrane Glycoproteins; D012333:RNA, Messenger / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000235:genetics*; D043562:Receptors, G-Protein-Coupled
D015342:DNA Probes; D010972:Platelet Activating Factor; D010980:Platelet Membrane Glycoproteins; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D043562:Receptors, G-Protein-Coupled; C039351:platelet activating factor receptor; D002118:Calcium