title: Homology of the envelope glycoprotein B of human herpesvirus-6 and cytomegalovirus.
abstract: The envelope glycoprotein B (gB) coding sequences of two strains of human herpesvirus-6 (HHV6 GS and Z29) were determined by sequencing a 2.5-kb open reading frame adjacent to the DNA polymerase sequence. The deduced primary translation product is 830 amino acids in length and is 96% conserved between the two divergent strains with no localized hypervariability noted. It contains the expected signal and transmembrane sequence motifs as well as a putative site of protease cleavage. There was 39% amino acid identity with the gB of human cytomegalovirus (CMV, strain AD169). HHV6-CMV gB peptide homology was evident through the entire sequence, but was especially strong in the amino-terminal portion of CMV gp55, which contains linear and conformational epitopes recognized by CMV-neutralizing antibodies. All 10 cysteine residues of HHV6 gB match corresponding residues of CMV gB. Sequence data suggest strong structural similarity and possible immunologic cross-reactivity of gB from the two viruses.
authors.affiliation: Medical Service, VA Medical Center, Portland, Oregon.
authors.forename: S
authors.initials: S
authors.lastname: Chou
authors.forename: G I
authors.initials: GI
authors.lastname: Marousek
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003587:Cytomegalovirus / Q000235:genetics*; D004279:DNA, Viral; D015654:Herpesvirus 6, Human / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000235:genetics*
chemical_list: D004279:DNA, Viral; D014759:Viral Envelope Proteins; C040495:glycoprotein B, Simplexvirus; C077311:glycoprotein B, human herpesvirus-6