"In vitro" effect of interleukin-1 beta on human glioma cell lines: regulation of cell proliferation and IL-6 production.
The human glioma cell lines U251 and HP591 were chosen as "in vitro" models for functional astrocytes. When cultured in the presence of IL-1 beta these cell lines demonstrated a marked increase in interleukin-6 production and in [3H]-thymidine uptake. The addition of dbcAMP could mimic the first effect of IL-1 beta but at the same time suppressed cell proliferation. These results suggest that IL-1 beta possibly exerts one of its biological effects (IL-6 synthesis) by means of the cyclic AMP pathway.
Cinque
S
S
Laboratory of Biochemistry IRC, K. U. Leuven Campus Kortrijk, Belgium.
Willems
J
J
Depraetere
S
S
Vermeire
L
L
Joniau
M
M
D003994:Bucladesine / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D000242:Cyclic AMP / Q000378:metabolism; D004261:DNA Replication / Q000187:drug effects; D005910:Glioma / Q000188:drug therapy* / Q000276:immunology / Q000473:pathology; D006801:Humans; D007375:Interleukin-1 / Q000627:therapeutic use*; D015850:Interleukin-6 / Q000096:biosynthesis*; D015398:Signal Transduction; D014407:Tumor Cells, Cultured
D007375:Interleukin-1; D015850:Interleukin-6; D003994:Bucladesine; D000242:Cyclic AMP