Vasopressin elevation of Na+/H+ exchange is inhibited by genistein in human blood platelets.
The regulation of intracellular Na+ and pHi in human blood platelets is known to be controlled by the function of the Na+/H+ exchanger. The phosphorylation state of the Na+/H+ exchanger which determines the exchanger activity in human blood platelets is regulated by the activities of protein kinases and protein phosphatases. Observations in this study indicate that arginine vasopressin (AVP) that interacts with a V1 receptor, activates the Na+/H+ exchange in human blood platelets through a genistein-inhibited mechanism. The AVP-activated Na+/H+ exchange is probably not regulated by protein kinase C (PKC), since this activation is not inhibited by staurosporine. The multiple ways in which platelet Na+/H+ exchange can be modulated may indicate the critical role played by this exchanger in the homeostasis control of pHi in human blood platelets.
Aharonovits
O
O
Department of Life Sciences, Ben-Gurion University of the Negev, Beer-Sheva, Israel.
Zik
M
M
Livne
A A
AA
Granot
Y
Y
D000328:Adult; D000470:Alkaloids / Q000494:pharmacology; D001127:Arginine Vasopressin / Q000494:pharmacology*; D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism*; D002352:Carrier Proteins / Q000097:blood*; D019833:Genistein; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007529:Isoflavones / Q000494:pharmacology*; D008875:Middle Aged; D010766:Phosphorylation; D011493:Protein Kinase C / Q000037:antagonists & inhibitors / Q000378:metabolism; D011522:Protons; D012964:Sodium / Q000097:blood; D017923:Sodium-Hydrogen Exchangers; D019311:Staurosporine; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D000470:Alkaloids; D002352:Carrier Proteins; D007529:Isoflavones; D011522:Protons; D017923:Sodium-Hydrogen Exchangers; D001127:Arginine Vasopressin; D012964:Sodium; D019833:Genistein; D011493:Protein Kinase C; D019311:Staurosporine; D013755:Tetradecanoylphorbol Acetate