title: Possible role of the submandibular glands in the control of glucose metabolism in mouse enterocytes.
abstract: We evaluated the effect of sialadenectomy on hexokinase activity and on rates of lactate formation and of [U-14C]glucose decarboxylation in 3 cellular fractions of the small intestine epithelium from male adult mice. The surgery was carried out under ether anesthesia and a sham-operated group was used as control. Three cell fractions were obtained by shaking the inverted small intestine: 1) tip of the villus, 2) villus and 3) villus and crypt cells. Five days after sialadenectomy, hexokinase activity was reduced in fractions 1 (3.53 +/- 0.65 vs 1.98 +/- 0.25 nmol min-1 mg protein-1, expressed as mean +/- SEM for 7 mice) and 3 (5.01 +/- 0.55 vs 3.15 +/- 0.42 nmol min-1 mg protein-1, mean +/- SEM for 7 mice). After removal of the submandibular glands, the rates of lactate formation were decreased in fractions 2 (4.16 +/- 0.54 vs 2.30 +/- 0.25, mean +/- SEM for 10 and 11 mice, respectively) and 3 (1.74 +/- 0.24 vs 0.87 +/- 0.14, mean +/- SEM for 13 mice) and the rates of [U-14C] glucose decarboxylation were reduced in fraction 1 (1.14 +/- 0.12 vs 0.61 +/- 0.10, mean +/- SEM for 11 and 12 mice, respectively). We conclude that the secretion of submandibular glands plays a physiological role in the control of glucose metabolism in enterocytes.
authors.affiliation: Departamento de Histologia e Embriologia, Universidade de São Paulo, Brasil.
authors.forename: M F
authors.initials: MF
authors.lastname: Santos
authors.forename: R
authors.initials: R
authors.lastname: Curi
authors.forename: F
authors.initials: F
authors.lastname: Fava-de-Moraes
mesh_terms: D000818:Animals; D003653:Decarboxylation; D004847:Epithelial Cells; D004848:Epithelium / Q000378:metabolism; D005947:Glucose / Q000378:metabolism*; D006593:Hexokinase / Q000378:metabolism; D007421:Intestine, Small / Q000166:cytology / Q000378:metabolism*; D007773:Lactates / Q000096:biosynthesis; D008297:Male; D051379:Mice; D013363:Submandibular Gland / Q000502:physiology* / Q000601:surgery; D013997:Time Factors
chemical_list: D007773:Lactates; D006593:Hexokinase; D005947:Glucose