Induction of differentiation of the human promyelocytic cell line HL-60 by activin/EDF.
A human promyelocytic cell line, HL-60, treated with activin/EDF was found to differentiate into monocyte/macrophage-like cells. This was shown not only by morphology but by the loss of myeloperoxidase granules and the appearance of nonspecific esterase. Dose-dependent inhibition of the differentiation by follistatin, an activin-binding protein, confirmed that it was indeed caused by activin. Thus, activin/EDF exerts its effect on hematopoietic cells not only on erythroid differentiation but also on at least a part of myeloid cell differentiation.
Yamada
R
R
Department of Biochemistry, Faculty of Medicine, Saitama Medical School, Japan.
Suzuki
T
T
Hashimoto
M
M
Eto
Y
Y
Shiokawa
K
K
Muramatsu
M
M
D028341:Activins; D001665:Binding Sites; D002454:Cell Differentiation / Q000187:drug effects; D003594:Cytoplasmic Granules / Q000648:ultrastructure; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D038681:Follistatin; D006023:Glycoproteins / Q000494:pharmacology; D006651:Histocytochemistry; D006801:Humans; D007265:Inhibins / Q000378:metabolism / Q000494:pharmacology*; D015473:Leukemia, Promyelocytic, Acute / Q000378:metabolism / Q000473:pathology*; D009195:Peroxidase / Q000378:metabolism; D014407:Tumor Cells, Cultured
D038681:Follistatin; D006023:Glycoproteins; D028341:Activins; D007265:Inhibins; D004815:Epidermal Growth Factor; D009195:Peroxidase