[Preparation of a monoclonal antibody against EA p138 of Epstein-Barr virus: its application in Sjogren's syndrome].
A monoclonal antibody against Epstein-Barr virus (EBV) encoded early antigen (EA) p138 was prepared with the use of a lysoprotein from the recombinant plasmid pUCARG1140. By using this specific monoclonal antibody and immunofluorescence as well as immunoenzyme methods, 4 of 13 lacrimal, 15 of 37 labial and all 7 renal biopsy tissues from patients with Sjogren's syndrome (SS) were found to have positive staining of epithelial cells, whereas those of control persons were all negative. These results suggest that EBV has been in the stage of active replication in the aforementioned tissues of SS and this virus may have a close association with SS in terms of pathogenesis.
Yang
J
J
PUMC Hospital, Beijing.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000956:Antigens, Viral / Q000032:analysis / Q000276:immunology*; D005260:Female; D004854:Herpesvirus 4, Human / Q000276:immunology* / Q000302:isolation & purification; D006801:Humans; D006825:Hybridomas; D007668:Kidney / Q000276:immunology; D007742:Labial Frenum / Q000276:immunology; D007765:Lacrimal Apparatus / Q000276:immunology; D051379:Mice; D012859:Sjogren's Syndrome / Q000276:immunology / Q000382:microbiology*
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral