title: Primary structure of the human elafin precursor preproelafin deduced from the nucleotide sequence of its gene and the presence of unique repetitive sequences in the prosegment.
abstract: The human elafin gene was cloned and its entire nucleotide sequence was determined to deduce the amino acid sequence for the precursor of elafin, an elastase-specific inhibitor. The gene spans approximately 1.7 kb and is divided into 3 exons. The gene product preproelafin consists of 117 amino acids: the initiator Met, a putative 25-amino acid signal peptide, a pro-sequence of about 34 amino acids, and the C-terminal 57 amino acids for mature elafin. Possible covalent clotting of the prosegment and its physiological significance have been pointed out based on a remarkable sequence similarity between the pro-sequence and the guinea pig seminal clotting protein SVP-1.
authors.affiliation: Department of Biological Sciences, Tokyo Institute of Technology, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Saheki
authors.forename: F
authors.initials: F
authors.lastname: Ito
authors.forename: H
authors.initials: H
authors.lastname: Hagiwara
authors.forename: Y
authors.initials: Y
authors.lastname: Saito
authors.forename: J
authors.initials: J
authors.lastname: Kuroki
authors.forename: S
authors.initials: S
authors.lastname: Tachibana
authors.forename: S
authors.initials: S
authors.lastname: Hirose
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D005091:Exons; D006801:Humans; D007438:Introns; D008969:Molecular Sequence Data; D011499:Protein Processing, Post-Translational; D021382:Protein Sorting Signals / Q000235:genetics*; D053491:Proteinase Inhibitory Proteins, Secretory; D011506:Proteins; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D015842:Serine Proteinase Inhibitors / Q000235:genetics*
chemical_list: D021382:Protein Sorting Signals; D053491:Proteinase Inhibitory Proteins, Secretory; D011506:Proteins; D015842:Serine Proteinase Inhibitors