A method for the assay of "difficult" interferons exemplified with recombinant equine interferon-beta 1.
We wished to assay recombinant equine interferon-beta 1 (rEqIFN-beta 1) but could not obtain satisfactory results with previously described methods. Therefore, we developed a yield-reduction assay, using primary horse peripheral blood mononuclear cells (PBMC) with vesicular stomatitis virus (VSV) for challenge, which proved consistently satisfactory and highly sensitive. It is suggested that this method of assay may be useful for IFNs from other animals where problems are encountered.
Marquardt
J
J
Immunology Unit, Veterinary School, Hannover, FRG.
Heinz
H
H
Heymer
J
J
Deegen
E
E
Adolf
G R
GR
Leibold
W
W
D000818:Animals; D001681:Biological Assay / Q000379:methods*; D006736:Horses; D016899:Interferon-beta / Q000032:analysis* / Q000494:pharmacology; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D011994:Recombinant Proteins / Q000032:analysis / Q000494:pharmacology; D014721:Vesicular stomatitis Indiana virus / Q000276:immunology*
D011994:Recombinant Proteins; D016899:Interferon-beta