Detection of CMV DNA in clinical samples of AIDS patients by chemiluminescence hybridization.
A sensitive chemiluminescence dot-blot hybridization assay for the detection of CMV DNA in clinical samples of AIDS patients is described. In the chemiluminescence hybridization assay, digoxigenin-labelled CMV DNA probes were used and when hybridized they were detected by anti-digoxigenin Fab fragments conjugated with alkaline phosphatase. Adamantyl 1,2-dioxetane phenyl phosphate was used as the chemiluminescent substrate. The results were recorded by instant photographic films. The results obtained with the chemiluminescence hybridization assay were compared with the results obtained by hybridization with colourimetric detection. The chemiluminescent assay proved specific, sensitive and reliable and thus can be used as a valuable routine diagnostic test for the detection of CMV DNA in clinical samples.
Musiani
M
M
Institute of Microbiology, University of Bologna, Italy.
Zerbini
M
M
Gentilomi
G
G
Gibellini
D
D
Gallinella
G
G
Venturoli
S
S
La Placa
M
M
D000163:Acquired Immunodeficiency Syndrome / Q000382:microbiology*; D003587:Cytomegalovirus / Q000302:isolation & purification*; D004279:DNA, Viral / Q000032:analysis* / Q000652:urine; D006801:Humans; D008163:Luminescent Measurements; D009693:Nucleic Acid Hybridization; D012463:Saliva / Q000382:microbiology; D012680:Sensitivity and Specificity
D004279:DNA, Viral