The QM gene is X-linked and therefore not involved in suppression of tumorigenesis in Wilms' tumor.
Inactivation of one or more tumor-suppressor genes on the short arm of chromosome 11 is thought to play a role in the etiology of Wilms' tumor. A candidate gene, QM, was recently isolated by subtractive hybridization between a tumorigenic cell line (deleted for part of 11p) and a non-tumorigenic cell line (the tumorigenic cell line carrying an extra t(X;11)copy). We show here with an exon-specific polymerase chain reaction that the genomic homolog of the QM cDNA is located in the G6PD-color vision genes region in Xq28. No homologous sequences could be detected on 11p. Our experiments indicate that the QM gene is not involved in the suppression of Wilms' tumor.
van den Ouweland
A M
AM
Department of Human Genetics, University Hospital Nijmegen, The Netherlands.
Verdijk
M
M
Mannens
M M
MM
van Oost
B A
BA
D001483:Base Sequence; D015139:Blotting, Southern; D005091:Exons / Q000235:genetics; D016147:Genes, Tumor Suppressor / Q000235:genetics*; D008040:Genetic Linkage / Q000235:genetics*; D006801:Humans; D006822:Hybrid Cells; D007680:Kidney Neoplasms / Q000235:genetics*; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes / Q000235:genetics; D016133:Polymerase Chain Reaction; D014407:Tumor Cells, Cultured; D009396:Wilms Tumor / Q000235:genetics*; D014960:X Chromosome
D015345:Oligonucleotide Probes