Conversion of an apparent 100 kDa folate binding protein from human milk, choroid plexus and semen to a 25 kDa molecular species by phosphatidylinositol-specific phospholipase C.
Gel filtration studies in the presence of Triton X-100 showed that treatment with phosphatidylinositol-specific phospholipase C reduced the apparent molecular size of the 100 kDa folate binding protein from human milk, choroid plexus and semen to 25 kDa. Cleavage of a hydrophobic glycosyl phosphatidylinositol domain (a membrane anchor) inserting the protein into Triton X-100 micelles could account for this phenomenon.
Hansen
S I
SI
Department of Clinical Chemistry, Central Hospital Hillerød, Denmark.
Holm
J
J
D002352:Carrier Proteins / Q000737:chemistry* / Q000378:metabolism; D002831:Choroid Plexus / Q000737:chemistry*; D002850:Chromatography, Gel; D058973:Folate Receptors, GPI-Anchored; D017261:Glycosylphosphatidylinositols; D008894:Milk Proteins / Q000737:chemistry* / Q000378:metabolism; D008895:Milk, Human / Q000737:chemistry*; D008970:Molecular Weight; D043265:Phosphatidylinositol Diacylglycerol-Lyase; D010716:Phosphatidylinositols / Q000737:chemistry; D054801:Phosphoinositide Phospholipase C; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism*; D011134:Polysaccharides / Q000737:chemistry; D011956:Receptors, Cell Surface; D012661:Semen / Q000737:chemistry*
D002352:Carrier Proteins; D058973:Folate Receptors, GPI-Anchored; D017261:Glycosylphosphatidylinositols; D008894:Milk Proteins; D010716:Phosphatidylinositols; D011134:Polysaccharides; D011956:Receptors, Cell Surface; D010727:Phosphoric Diester Hydrolases; D054801:Phosphoinositide Phospholipase C; D043265:Phosphatidylinositol Diacylglycerol-Lyase