Lipid peroxidation in human breast cancer cells in response to gamma-linolenic acid and iron.
Lipid peroxidation in human breast cancer (ZR-75-1) cells and cancer cell killing were confirmed by using ultraviolet (UV)-spectrophotometry and mass spectrometry (MS). ZR-75-1 cells and human normal fibroblast CCD-41-SK (41Sk) cells were cultured with gamma-linolenic acid (GLA) and ferrous iron Fe (II) combinations. Formation of lipid peroxide and cytotoxic effect were highest in ZR-75-1 cells treated with GLA + Fe (II), though 41Sk cells showed little evidence of either lipid peroxidation or cytotoxity. These results indicate a cancer-cell-specific lipid peroxidation mechanism in association with the selective cancer cell killing effect in response to GLA.
Takeda
S
S
Efamnol Research Institute, Kentville, Nova Scotia, Canada.
Horrobin
D F
DF
Manku
M
M
Sim
P G
PG
Ells
G
G
Simmons
V
V
D000970:Antineoplastic Agents / Q000494:pharmacology*; D001943:Breast Neoplasms / Q000378:metabolism* / Q000473:pathology; D002470:Cell Survival / Q000187:drug effects; D005260:Female; D006801:Humans; D007501:Iron / Q000494:pharmacology*; D008042:Linolenic Acids / Q000494:pharmacology*; D015227:Lipid Peroxidation / Q000187:drug effects*; D014407:Tumor Cells, Cultured; D017965:gamma-Linolenic Acid
D000970:Antineoplastic Agents; D008042:Linolenic Acids; D017965:gamma-Linolenic Acid; D007501:Iron