title: Correlation in the expression of type IV collagenase and the invasive and chemotactic abilities of myelomonocytic cells during differentiation into macrophages.
abstract: Monomyelocytic phagocytes originate in the bone marrow and while differentiating into macrophages migrate to inflammatory foci and target tissues by egress from the capillary blood vessels. During such diapedesis, the cells must traverse tissue barriers such as basement membrane, which has type IV collagen as its principal structural element. We studied whether the expression of type IV collagenase activity, invasion through basement membrane, and the response to inflammatory chemoattractants are related to each other and to the process of differentiation of murine M1 myeloid leukemia cells into macrophages. M1 cells stimulated with mouse lung-conditioned medium (MLCM) or interleukin 6 (IL6) differentiate into macrophages by 72 h, as determined by expression of Fc receptors, induction of lysozyme, and morphological changes from blast cells to mature macrophages. During this process of differentiation the invasive ability of the cells and the amount of type IV collagenase in the supernatants from the invading cells continuously increased up to 72 h. Zymographic analysis of supernatants of the invading cells revealed a single 100-kd metalloproteinase with gelatinolytic activity. Chemotaxis towards arachidonic acid metabolites, which are present in inflamed tissues, was detected only in differentiated cells. Studies with thioglycolate (TG)-elicited peritoneal macrophages gave results similar to those obtained with differentiated M1 cells, showing that the ability to invade basement membrane, the expression of type IV collagenase, and the chemotactic response to inflammatory chemoattractants all increased with the differentiation of myeloid cells and reached their highest expression in fully differentiated cells.
authors.affiliation: Division of Cytokine Biology, CBER, FDA, Bethesda, MD 20892.
authors.forename: D H
authors.initials: DH
authors.lastname: Pluznik
authors.forename: R
authors.initials: R
authors.lastname: Fridman
authors.forename: R
authors.initials: R
authors.lastname: Reich
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D002465:Cell Movement / Q000502:physiology; D002633:Chemotaxis / Q000187:drug effects / Q000502:physiology*; D003470:Culture Media / Q000494:pharmacology; D006412:Hematopoietic Stem Cells / Q000201:enzymology* / Q000473:pathology* / Q000502:physiology*; D015850:Interleukin-6 / Q000494:pharmacology; D007951:Leukemia, Myeloid / Q000201:enzymology / Q000473:pathology / Q000503:physiopathology; D008264:Macrophages / Q000473:pathology*; D051379:Mice; D003012:Microbial Collagenase / Q000032:analysis*; D010586:Phagocytes / Q000166:cytology* / Q000201:enzymology* / Q000502:physiology; D013864:Thioglycolates / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000201:enzymology / Q000473:pathology
chemical_list: D003470:Culture Media; D015850:Interleukin-6; D013864:Thioglycolates; D003012:Microbial Collagenase