title: Rearrangement of viral sequences in cytopathogenic pestiviruses.
abstract: Two cytopathogenic isolates of bovine viral diarrhea virus (cpBVDV) have been analyzed. For both viruses two regions of their genomic RNAs were found to be duplicated and rearranged. The viral genomes contain a small duplicated element (SD) derived from the genomic 5' end far downstream of its original context. This sequence is followed by a larger duplication which encompasses the region coding for the protein p80(LD), a molecular marker for cpBVDV. The SD element codes for the viral protease p20. In the case of the viruses analyzed here the aminoterminus of p80 is generated by autoproteolytic removal of the preceding SD-encoded protease. For one of the cpBVDV isolates a specific fusion protein (p28) could be identified which is composed of p20 and part of p10, another viral protein. Molecular characterization of the respective noncytopathogenic counterpart revealed that duplication and rearrangement of sequences as well as the expression of p28 and p80 are specific for the cytopathogenic virus.
authors.affiliation: Federal Research Centre for Virus Diseases of Animals, Tübingen, Federal Republic of Germany.
authors.forename: G
authors.initials: G
authors.lastname: Meyers
authors.forename: N
authors.initials: N
authors.lastname: Tautz
authors.forename: R
authors.initials: R
authors.lastname: Stark
authors.forename: J
authors.initials: J
authors.lastname: Brownlie
authors.forename: E J
authors.initials: EJ
authors.lastname: Dubovi
authors.forename: M S
authors.initials: MS
authors.lastname: Collett
authors.forename: H J
authors.initials: HJ
authors.lastname: Thiel
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000382:microbiology; D002417:Cattle; D002460:Cell Line; D004279:DNA, Viral; D001908:Diarrhea Viruses, Bovine Viral / Q000235:genetics* / Q000472:pathogenicity; D016679:Genome, Viral; D008969:Molecular Sequence Data; D011233:Precipitin Tests; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D011995:Recombination, Genetic; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D004279:DNA, Viral; D011993:Recombinant Fusion Proteins; D014764:Viral Proteins