Target recognition by bovine mononuclear, MHC-unrestricted cytotoxic cells.
A population of bovine non B/non T, cytotoxic lymphocytes with natural killer activity against virus-infected and non-infected embryonic kidney cells was functionally characterized. The data obtained in experiments of flow cytometry and immuno-peroxidase staining show that a CD2-, CD4-, CD8-, TcR gamma delta-, CD3+, CD45+, FcR+ lymphoid killer cell does exist within bovine peripheral blood leucocytes. This population can detect the down-regulation of class I MHC antigens or the expression of embryonic forms thereof, as shown by experiments of 17-hour 51Cr release and binding to target cells. This model was tested in vitro in experiments on virus-infected bovine kidney cells. The emerging picture was substantially in agreement with the "missing self" theory as a possible option for target cell recognition. In this respect, the profound alteration of MHC Class I expression could represent a major early event, recognized on virus-infected cells by the immune system.
Amadori
M
M
Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia, Brescia, Italy.
Archetti
I L
IL
Verardi
R
R
Berneri
C
C
D000256:Adenoviridae / Q000276:immunology; D000818:Animals; D002417:Cattle / Q000276:immunology*; D002460:Cell Line; D002478:Cells, Cultured; D003602:Cytotoxicity, Immunologic; D015536:Down-Regulation; D004770:Enterovirus / Q000276:immunology; D005260:Female; D005434:Flow Cytometry; D007242:Herpesvirus 1, Bovine / Q000276:immunology; D015395:Histocompatibility Antigens Class I / Q000096:biosynthesis / Q000276:immunology*; D007124:Immunoenzyme Techniques; D007694:Killer Cells, Natural / Q000276:immunology*; D008214:Lymphocytes / Q000276:immunology*; D010224:Parainfluenza Virus 3, Human / Q000276:immunology; D018406:Virus Physiological Phenomena; D014780:Viruses / Q000276:immunology
D015395:Histocompatibility Antigens Class I