Coordination of ATP production and consumption in brain: parallel regulation of cytochrome oxidase and Na+, K(+)-ATPase.
Previous studies have shown that most neuronal ATP is produced by oxidative metabolism, and consumed by Na+,K(+)-ATPase. We hypothesized that the distribution of Na+,K(+)-ATPase in brain would correlate with that of the energy-producing enzyme, cytochrome oxidase (CO). We localized these enzymes in monkey hippocampus and striate cortex by histochemistry and immunohistochemistry. Their distributions were generally similar, although some differences were observed. We also studied regulation of enzyme levels, using monocular impulse blockage with tetrodotoxin (TTX) to alter neuronal activity in the visual system. Parallel changes in CO and Na+,K(+)-ATPase activity were induced in striate cortex. These results provide further evidence that neuronal energy demands regulate CO levels and distribution.
Hevner
R F
RF
Department of Cellular Biology and Anatomy, Medical College of Wisconsin, Milwaukee 53226.
Duff
R S
RS
Wong-Riley
M T
MT
D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D003576:Electron Transport Complex IV / Q000378:metabolism*; D006624:Hippocampus / Q000187:drug effects / Q000201:enzymology* / Q000473:pathology; D006651:Histocytochemistry; D006706:Homeostasis; D007150:Immunohistochemistry; D007527:Isoenzymes / Q000378:metabolism*; D008252:Macaca fascicularis; D008255:Macaca radiata; D009474:Neurons / Q000187:drug effects / Q000201:enzymology* / Q000473:pathology; D012016:Reference Values; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*; D013779:Tetrodotoxin / Q000633:toxicity; D014793:Visual Cortex / Q000187:drug effects / Q000201:enzymology* / Q000473:pathology
D007527:Isoenzymes; D013779:Tetrodotoxin; D000255:Adenosine Triphosphate; D003576:Electron Transport Complex IV; D000254:Sodium-Potassium-Exchanging ATPase