[A highly sensitive diagnostic kit for evaluating therapeutic effect in schistosomiasis cases].
A highly sensitive diagnostic kit for detecting circulating antigens of schistosomiasis was developed. It can detect the circulating antigen of this infection at a level of 10(-9) g/ml for the purposes of diagnosis and treatment. In acute cases of schistosomiasis, the detecting rate was 100% in 45 cases, and 93.0%-100% in 621 cases of early chronic stage with various infection loads. False positive rate was 0.1% in 513 normal individuals and 438 cases of other parasitic infections. The results showed that the kit was of high sensitivity and specificity. The variation coefficients in single batch and between batches were found to be less than 10%, indicating its good stability and reproducibility. Serum samples from cases treated by praziquantel were tested with the kit with a negative reaction rate of 79.7% 3 months after treatment. The kit can be used for diagnostic and therapeutic purposes.
Wang
X Z
XZ
Institute of Antiparasitic Diseases, Sichuan Academy of Medical Sciences, Chengdu.
D000818:Animals; D000947:Antigens, Helminth / Q000097:blood*; D005069:Evaluation Studies as Topic; D006801:Humans; D011933:Reagent Kits, Diagnostic / Q000592:standards*; D012549:Schistosoma japonicum / Q000276:immunology*; D012554:Schistosomiasis japonica / Q000175:diagnosis*; D012680:Sensitivity and Specificity
D000947:Antigens, Helminth; D011933:Reagent Kits, Diagnostic