title: Measurement of isotype-specific antibody responses to Aujeszky's disease virus in sera and mucosal secretions of pigs.
abstract: Enzyme-linked immunosorbent assays (ELISAs) for the detection of porcine IgM, IgA, IgG1 and IgG2 antibodies directed against Aujeszky's disease virus (ADV) are described. ADV-specific IgA and IgM were detected in an antibody capture assay, and ADV-specific IgG1 and IgG2 were detected in an indirect double antibody sandwich assay. A selected set of samples was tested in the four ELISAs and in a 24 h virus neutralization assay. Comparison of the results showed that the ELISAs were isotype-specific, sensitive, and reproducible. Samples with ADV antibody of one isotype showed that ADV-specific IgG1, IgG2 and IgM were able to neutralize the virus in vitro. In vitro neutralization of virus can be enhanced by complement. ADV-specific IgA neutralized virus only weakly. ADV-infected cells activated complement in the absence of antibody. Specific IgG2 and IgM enhanced complement activation. Analysis of the time course of antibody responses after infection or vaccination revealed that the isotype-specific ELISAs are suitable to study the humoral antibody response of pigs to the virus in mucosal secretions. Wild-type virus (strain NIA-3) and an attenuated vaccine strain (Bartha) administered intranasally induced mucosal IgM and IgA responses to the virus. In contrast, a killed vaccine (Nobivac) administered intramuscularly induced only weak mucosal IgM responses. The attenuated vaccine strain primed for a mucosal IgA memory response evoked upon challenge infection with wild-type virus.
authors.affiliation: Central Veterinary Institute, Department of Virology, Lelystad, Netherlands.
authors.forename: T G
authors.initials: TG
authors.lastname: Kimman
authors.forename: R A
authors.initials: RA
authors.lastname: Brouwers
authors.forename: F J
authors.initials: FJ
authors.lastname: Daus
authors.forename: J T
authors.initials: JT
authors.lastname: van Oirschot
authors.forename: D
authors.initials: D
authors.lastname: van Zaane
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D000956:Antigens, Viral / Q000276:immunology; D003167:Complement Activation / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D011558:Herpesvirus 1, Suid / Q000276:immunology*; D007132:Immunoglobulin Isotypes / Q000032:analysis*; D009297:Nasal Mucosa / Q000276:immunology*; D009500:Neutralization Tests; D011557:Pseudorabies / Q000276:immunology* / Q000517:prevention & control; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013552:Swine; D013997:Time Factors; D014611:Vaccination; D014612:Vaccines / Q000008:administration & dosage
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D007132:Immunoglobulin Isotypes; D014612:Vaccines