Evaluation of human papillomavirus type 5 on frozen sections of multiple lesions from transplant recipients with in situ hybridization and non-isotopic probes.
Transplant recipients are at high risk to develop multiple cutaneous lesions after grafting. The frequency of the potentially oncogenic human papillomavirus (HPV) type 5 DNA was evaluated in cutaneous lesions taken from sun-exposed areas in transplant recipients (92 lesions and 5 samples from normal skin) and compared with a nontransplanted population (22 lesions and 7 samples from normal skin) using in situ hybridization and biotinylated probes to HPV types 1, 2, 5, 16 and 18. HPV type 5 DNA was identified in 8/92 cutaneous lesions of transplanted recipients: 3 warts, 1 case of seborrheic keratosis, 2 actinic keratoses and 2 keratoacanthomas. HPV type 5 DNA was not detected in 27 malignant tumors (8 basal cell carcinomas and 19 squamous cell carcinomas) from transplant recipients. HPV DNA type 5 was detected in only 1 case of squamous cell carcinoma from the general population. The presence of HPV DNA 5 was confirmed with Southern blotting in 2 out of 6 cases from transplant recipients. The reaction was negative with the squamous cell carcinoma from nontransplant recipients. These data indicate that the presence of HPV DNA type 5 is not very frequent; it can be detected with in situ hybridization and nonisotopic probe, which is easier to handle than Southern blot.
Soler
C
C
INSERM U 209, Hôpital E.-Herriot, Lyon, France.
Chardonnet
Y
Y
Euvrard
S
S
Chignol
M C
MC
Thivolet
J
J
D001706:Biopsy; D015344:DNA Probes, HPV; D005629:Frozen Sections; D006801:Humans; D016030:Kidney Transplantation / Q000009:adverse effects*; D027383:Papillomaviridae / Q000302:isolation & purification*; D012867:Skin / Q000473:pathology*; D012874:Skin Diseases, Infectious / Q000382:microbiology* / Q000473:pathology; D012878:Skin Neoplasms / Q000382:microbiology* / Q000473:pathology; D014412:Tumor Virus Infections / Q000382:microbiology* / Q000473:pathology
D015344:DNA Probes, HPV