Status of the ICP34.5 gene in herpes simplex virus type 1 strain 17.
In the published DNA sequence of herpes simplex virus type 1 (HSV-1) strain 17 the coding region for the neurovirulence factor ICP34.5 is disrupted by a 2 bp insertion relative to the corresponding sequence in HSV-1 strain F; this difference would render 60% of the ICP34.5 coding sequence out of frame in strain 17. Re-investigation of the strain 17 sequence showed that the plasmid clone used as the major sequencing substrate for the region was atypical in that other clones did not possess the 2 bp insertion. It was concluded that the coding sequence for ICP34.5 is intact in HSV-1 strain 17 and that the HSV-1 DNA sequence should be revised at this locus.
Dolan
A
A
MRC Virology Unit, University of Glasgow, U.K.
McKie
E
E
MacLean
A R
AR
McGeoch
D J
DJ
D001483:Base Sequence; D003001:Cloning, Molecular; D004251:DNA Transposable Elements / Q000235:genetics*; D005814:Genes, Viral / Q000235:genetics*; D014644:Genetic Variation; D008969:Molecular Sequence Data; D016364:Reading Frames; D018139:Simplexvirus / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics*
D004251:DNA Transposable Elements; D014764:Viral Proteins; C066469:gamma 34.5 protein, Human herpesvirus 1