title: Influence of fixation on human papillomavirus DNA detection in frozen and embedded paraffin lesions by in situ hybridization with biotinylated probes.
abstract: Series of frozen or paraffin-embedded tissues from various body sites, taken from non-immunosuppressed or immunosuppressed patients with persistent papilloma lesions were examined for the presence of group specific antigen from human papillomavirus (HPV) by indirect immunofluorescence or HPV DNA by in situ hybridization with biotinylated probes. We have shown that it is possible to detect HPV DNA after fixation of tissues in neutral formalin, Bouin's or Baker's solution. However, the sensitivity was reduced as compared to frozen tissues. The HPV DNA was detected in nuclei of heavily infected epithelial cells such as plantar or hand warts or in dispersed cells containing high copy numbers of HPV DNA from lesions such as squamous cell carcinomas or keratoacanthomas. In premalignant or malignant lesions of both immunosuppressed or non-immunosuppressed patients, HPV DNA was rarely detected after fixation. HPV types commonly described for skin and genital samples were identified in non-immunosuppressed patients, whereas in transplant recipients oncogenic HPV type 16 was identified in benign warts as well as in premalignant or malignant lesions. Positive reactions with several HPV types were more frequent in lesions from grafted patients than from the normal population. Virus antigen was detectable more frequently in frozen sections than in fixed tissues. Our findings indicate that in situ hybridization is an appropriate and rapid technique to study the presence of HPV infection. However, numerous controls are needed to avoid misinterpretations.
authors.affiliation: INSERM U209, Hôpital E. Herriot, Lyon, France.
authors.forename: C
authors.initials: C
authors.lastname: Soler
authors.forename: Y
authors.initials: Y
authors.lastname: Chardonnet
authors.forename: I
authors.initials: I
authors.lastname: Guerin-Reverchon
authors.forename: M C
authors.initials: MC
authors.lastname: Chignol
authors.forename: J
authors.initials: J
authors.lastname: Viac
authors.forename: S
authors.initials: S
authors.lastname: Euvrard
authors.forename: J
authors.initials: J
authors.lastname: Thivolet
mesh_terms: D000956:Antigens, Viral / Q000032:analysis*; D002277:Carcinoma / Q000276:immunology / Q000382:microbiology; D015925:Cryopreservation; D015344:DNA Probes, HPV; D004279:DNA, Viral / Q000302:isolation & purification*; D006801:Humans; D016867:Immunocompromised Host; D017403:In Situ Hybridization; D027383:Papillomaviridae / Q000302:isolation & purification*; D016612:Paraffin Embedding; D012680:Sensitivity and Specificity; D016707:Tissue Fixation / Q000379:methods*; D014412:Tumor Virus Infections / Q000276:immunology / Q000382:microbiology*; D014860:Warts / Q000276:immunology / Q000382:microbiology
chemical_list: D000956:Antigens, Viral; D015344:DNA Probes, HPV; D004279:DNA, Viral