cAMP control of Mg2+ homeostasis in heart and liver cells.
The increase of intracellular cAMP level in intact myocytes and hepatocytes induced by beta-adrenergic stimulation or by forskolin or permeant cyclic AMP analogues induces a sizeable Mg2+ efflux from the cells. Approximately 10-15% of total cell Mg2+ is mobilized over 5-8 min stimulation. The addition of cAMP to digitonin-permeabilized hepatocytes also mobilizes Mg2+ from intracellular compartment(s). Furthermore, cAMP stimulates a major Mg2+ extrusion from isolated rat liver mitochondria, indicating this compartment as the main source of Mg2+ mobilization in liver cells.
Romani
A
A
Department of Physiology and Biophysics, Case Western Reserve University, Cleveland, Ohio.
Scarpa
A
A
D000818:Animals; D001692:Biological Transport; D002463:Cell Membrane Permeability / Q000187:drug effects; D005576:Colforsin / Q000494:pharmacology; D000242:Cyclic AMP / Q000031:analogs & derivatives / Q000502:physiology*; D004072:Digitonin / Q000494:pharmacology; D006321:Heart / Q000187:drug effects; D006706:Homeostasis / Q000502:physiology; D008099:Liver / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D008274:Magnesium / Q000378:metabolism*; D008297:Male; D008930:Mitochondria, Liver / Q000187:drug effects / Q000378:metabolism; D000226:Mitochondrial ADP, ATP Translocases / Q000378:metabolism; D009206:Myocardium / Q000166:cytology / Q000378:metabolism*; D009638:Norepinephrine / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley / Q000502:physiology
D005576:Colforsin; D000226:Mitochondrial ADP, ATP Translocases; D000242:Cyclic AMP; D008274:Magnesium; D004072:Digitonin; D009638:Norepinephrine