Detection of human papillomavirus in cervical scrapes by the polymerase chain reaction in relation to cytology: possible implications for cervical cancer screening.
An HPV screening strategy based on general primer-mediated and type-specific PCR is described. A relationship was found between an increasing HPV prevalence rate and a higher degree of cervical dysplasia (up to 100% in cervical carcinoma). Based on these prevalence studies in the Netherlands and preliminary data showing that progression of cervical lesions is always associated with persistent infection of oncogenic/high-risk HPV types, a cervical cancer screening scheme incorporating PCR-based HPV detection is proposed for use in industrialized countries.
Meijer
C J
CJ
Department of Pathology, Free University Hospital, Amsterdam, The Netherlands.
van den Brule
A J
AJ
Snijders
P J
PJ
Helmerhorst
T
T
Kenemans
P
P
Walboomers
J M
JM
D002584:Cervix Uteri / Q000382:microbiology* / Q000473:pathology*; D005260:Female; D006801:Humans; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D014412:Tumor Virus Infections / Q000175:diagnosis* / Q000473:pathology*; D002583:Uterine Cervical Neoplasms / Q000175:diagnosis* / Q000382:microbiology / Q000473:pathology*; D014626:Vaginal Smears