Humoral response in mice immunized with outer membrane proteins of Hafnia alvei. Protective activity of anti-OMP-antibodies.
Intraperitoneal immunization of mice with outer membrane proteins (OMP) of Hafnia alvei induced in the animals a synthesis of specific antibodies. The antibody levels, determined by ELISA test, were found to be relatively low in the sera of mice immunized with a single dose (5 micrograms) of OMP and after a second immunization. However, they were higher in mice immunized with three doses of OMP. The antibodies were present in circulation for a relatively short time after immunization. Serum containing anti-OMP antibodies given intraperitoneally to normal mice protected them only against challenge with a homologous Hafnia strain.
Witkowska
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Laboratory of Medical Microbiology, Polish Academy of Sciences, Wrocław.
Czarny
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Mulczyk
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D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis*; D000942:Antigens, Bacterial / Q000008:administration & dosage; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology*; D004755:Enterobacteriaceae / Q000276:immunology*; D007115:Immunization Schedule; D007116:Immunization, Passive; D007700:Kinetics; D051379:Mice; D008815:Mice, Inbred Strains; D014611:Vaccination
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins