Surface lysine and tyrosine residues are required for interaction of the major herpes simplex virus type 1 DNA-binding protein with single-stranded DNA.
Modification of the herpes simplex virus type 1 major DNA-binding protein (ICP8) with reagents and conditions specific for arginine, lysine, and tyrosine residues indicates that surface lysine and tyrosine residues are required for the interaction of this protein with single-stranded DNA. Modification of either of these two amino acids resulted in a loss and/or modification of binding activity as judged by nitrocellulose filter assays and gel shift. Modification specific for arginine residues did not affect binding within the limits of the assays used. Finally, quenching of the intrinsic tryptophan fluorescence of ICP8 in the presence of single-stranded DNA either suggests involvement of this amino acid in the binding reaction or reflects a conformational change in the protein upon binding.
Ruyechan
W T
WT
Department of Microbiology, School of Medicine, State University of New York, Buffalo 14214.
Olson
J W
JW
D000089:Acetic Anhydrides / Q000494:pharmacology; D001120:Arginine / Q000031:analogs & derivatives / Q000378:metabolism; D001665:Binding Sites; D004277:DNA, Single-Stranded / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000187:drug effects / Q000378:metabolism*; D005453:Fluorescence; D007455:Iodine / Q000494:pharmacology; D008239:Lysine / Q000031:analogs & derivatives / Q000378:metabolism; D010658:Phenylglyoxal / Q000494:pharmacology; D018139:Simplexvirus / Q000737:chemistry*; D013499:Surface Properties; D014443:Tyrosine / Q000031:analogs & derivatives / Q000378:metabolism; D014764:Viral Proteins / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*
D000089:Acetic Anhydrides; D004277:DNA, Single-Stranded; D004268:DNA-Binding Proteins; C045462:ICP8 protein, Simplexvirus; D014764:Viral Proteins; C031800:acetic anhydride; D014443:Tyrosine; D001120:Arginine; D007455:Iodine; D008239:Lysine; D010658:Phenylglyoxal