title: Electrophysiological evidence for the presence of ionotropic and metabotropic excitatory amino acid receptors on dopaminergic neurons of the rat mesencephalon: an in vitro study.
abstract: The actions of the ionotropic and metabotropic excitatory amino acid agonists AMPA, quisqualate, kainate, NMDA and trans-ACDP were studied by means of intracellular electrophysiological recordings from dopaminergic neurons of rat mesencephalon in brain slices. It was observed that all these agents evoked an inward current in cells which were voltage-clamped near the resting potential (-50, -60 mV). The membrane responses produced by AMPA, kainate and quisqualate were associated with an increase of the apparent input conductance while the responses induced by NMDA and trans-ACDP were associated with a decrease in the apparent input conductance. Therefore, stimulation of ionotropic and metabotropic amino acid receptors activates inward currents in the dopaminergic cells by different mechanisms.
authors.affiliation: Neurological Clinic, 2nd University of Rome Tor Vergata, Italy.
authors.forename: N B
authors.initials: NB
authors.lastname: Mercuri
authors.forename: F
authors.initials: F
authors.lastname: Stratta
authors.forename: P
authors.initials: P
authors.lastname: Calabresi
authors.forename: G
authors.initials: G
authors.lastname: Bernardi
mesh_terms: D000818:Animals; D046508:Culture Techniques; D004298:Dopamine / Q000502:physiology*; D005260:Female; D008297:Male; D008564:Membrane Potentials / Q000502:physiology; D008636:Mesencephalon / Q000502:physiology*; D009474:Neurons / Q000502:physiology; D051381:Rats; D011919:Rats, Inbred Strains; D018091:Receptors, AMPA; D017459:Receptors, Amino Acid; D011956:Receptors, Cell Surface / Q000502:physiology*; D011954:Receptors, Dopamine / Q000502:physiology*; D018092:Kainic Acid Receptors; D016194:Receptors, N-Methyl-D-Aspartate / Q000502:physiology*; D017981:Receptors, Neurotransmitter / Q000502:physiology; D013378:Substantia Nigra / Q000502:physiology; D009435:Synaptic Transmission / Q000502:physiology*; D013681:Tegmentum Mesencephali / Q000502:physiology
chemical_list: D004298:Dopamine; D018091:Receptors, AMPA; D017459:Receptors, Amino Acid; D011956:Receptors, Cell Surface; D011954:Receptors, Dopamine; D018092:Kainic Acid Receptors; D016194:Receptors, N-Methyl-D-Aspartate; D017981:Receptors, Neurotransmitter