Type 1 pili enhance the invasion of Salmonella braenderup and Salmonella typhimurium to HeLa cells.
The relationship between type 1 pili-associated adhesion and invasion to HeLa cells by Salmonella braenderup and S. typhimurium was studied. When the clinical isolates of these strains were grown in L-broth, they showed both type 1 pili formation and mannose-sensitive adhesion to HeLa cells. On the other hand, the type 1 pili-defective mutants, which were obtained either by repeated subcultures on L-agar plates or by the transposon Tn1-insertion mutagenesis of the S. braenderup and S. typhimurium strains, concomitantly lost mannose-sensitive adhesion to HeLa cells. When the HeLa cells were incubated with Salmonella, the type 1 piliated strains invaded the HeLa cells with much higher infection rate than did the type 1 pili-defective strains. The invasion of type 1 piliated strains to HeLa cells was markedly inhibited in the presence of D-mannose. The infectivity of the strain, which lost type 1 pili but still had mannose-resistant adhesion, was slightly higher than that of the strains defective in both mannose-sensitive and mannose-resistant adhesion. These results suggested that type 1 pili have a role in enhancing the invasion of S. braenderup and S. typhimurium to HeLa cells.
Horiuchi
S
S
Department of Microbiology, Faculty of Medicine, Tokyo Medical and Dental University, Japan.
Inagaki
Y
Y
Okamura
N
N
Nakaya
R
R
Yamamoto
N
N
D000208:Acute Disease; D000372:Agglutination Tests; D000818:Animals; D001422:Bacterial Adhesion / Q000187:drug effects; D004760:Enterocolitis / Q000382:microbiology; D010861:Fimbriae, Bacterial / Q000502:physiology* / Q000648:ultrastructure; D006367:HeLa Cells / Q000382:microbiology / Q000648:ultrastructure; D006801:Humans; D008358:Mannose / Q000494:pharmacology; D016254:Mutagenesis, Insertional; D011817:Rabbits; D012475:Salmonella / Q000502:physiology* / Q000648:ultrastructure; D012486:Salmonella typhimurium / Q000502:physiology* / Q000648:ultrastructure
D008358:Mannose