An interaction between p21ras and heat shock protein hsp60, a chaperonin.
Ras proteins play a crucial role in the development of neoplasia and in signal transduction in normal cells. In a search for proteins interacting with p21ras, we previously identified a protein of 60 kDa (p60) through use of a chemical cross-linker. Using information from partial amino acid sequencing of the purified protein, we isolated full-length cDNA clones encoding this 60-kDa protein. Nucleotide sequence analysis revealed that p60 is the murine heat shock protein hsp60, a chaperonin. Association of hsp60 with p21ras appears physiological, as the amount of hsp60 complexed to p21ras was similar even in cells over-expressing p21ras, and reversing the order of cross-linking and lysis of the cells, which releases large amounts of hsp60 from mitochondria, did not alter the amount of hsp60 cross-linked to p21ras.
Ikawa
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Whitehead Institute for Biomedical Research, Cambridge, MA 02142.
Weinberg
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D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D001483:Base Sequence; D002460:Cell Line; D018833:Chaperonins; D003001:Cloning, Molecular; D003432:Cross-Linking Reagents; D006360:Heat-Shock Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D016283:Proto-Oncogene Proteins p21(ras) / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D012689:Sequence Homology, Nucleic Acid; D013388:Succinimides
D000911:Antibodies, Monoclonal; D003432:Cross-Linking Reagents; D006360:Heat-Shock Proteins; D009838:Oligodeoxyribonucleotides; D011506:Proteins; D013388:Succinimides; D018833:Chaperonins; C501009:HRAS protein, human; D016283:Proto-Oncogene Proteins p21(ras); C011240:dithiobis(succinimidylpropionate)