Trisomy and chromosome size changes in hybrid trypanosomes from a genetic cross between Trypanosoma brucei rhodesiense and T. b. brucei.
Further analysis of hybrid clones from an experimental cross of Trypanosoma brucei rhodesiense 058 and T. b. brucei 196 shows 2 of the hybrid clones to have DNA contents about 1.5 times parental values. This represents over 40,000 kb of extra DNA. Comparison of the molecular karyotypes of parental and progeny trypanosomes shows that the bulk of the extra DNA constitutes chromosomes greater than 1 Mb in size, although a small proportion can be accounted for by an increased number of mini-chromosomes. The 2 hybrid clones have 3 alleles at several loci for housekeeping genes as shown by RFLP and isoenzyme analysis. Trisomy of the chromosome carrying phosphoglycerate kinase and tubulin genes and that carrying the phospholipase C gene was demonstrated by analysis of molecular karyotypes. These chromosomes appear prone to substantial size alterations associated with genetic exchange. Our results for one of the hybrid clones are completely consistent with it being triploid and the product of fusion of haploid and diploid nuclei.
Gibson
W
W
Department of Pathology and Microbiology, University of Bristol Veterinary School, Langford, UK.
Garside
L
L
Bailey
M
M
D000483:Alleles; D000818:Animals; D002875:Chromosomes / Q000648:ultrastructure*; D016054:DNA, Protozoan / Q000032:analysis; D006824:Hybridization, Genetic; D007521:Isocitrate Dehydrogenase / Q000235:genetics / Q000378:metabolism; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D007621:Karyotyping; D051379:Mice; D012150:Polymorphism, Restriction Fragment Length; D015183:Restriction Mapping; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism; D014314:Trisomy; D014346:Trypanosoma brucei brucei / Q000201:enzymology / Q000235:genetics*; D016833:Trypanosoma brucei rhodesiense / Q000201:enzymology / Q000235:genetics*
D016054:DNA, Protozoan; D007527:Isoenzymes; D007521:Isocitrate Dehydrogenase; D013482:Superoxide Dismutase