Bacterial contamination and the effect of filters in anaesthetic circuits in a simulated patient model.
In order to investigate bacterial contamination of anaesthetic breathing circuits and means of prevention of this, six different laboratory experiments were performed. These experiments involved the bacterial contamination of Dräger Narkose Spiromat 650 and Dräger AV-1 circle system circuits and of an isolated soda lime carbon dioxide absorber. The effects of anaesthetic gas, gas flow rate and the incorporation of a hydrophobic membrane heat and moisture exchanging bacterial/viral filter (HMEF) at the patient end of these circuits were investigated. It was found that without a HMEF the whole interior of the anaesthetic circuits became contaminated with bacteria. Components closest to the simulated patient showed the highest levels of contamination. Higher gas flows were associated with decreased levels of circuit contamination, presumably because more bacteria were expelled from the system. Halothane (1 volume %) and soda lime were not found to have any demonstrable bactericidal action. The presence of a HMEF between the simulated patient and the Y-piece prevented any detectable contamination from reaching the circuit. Consequently, the presence of a HMEF provides protection of the anaesthetic circuit as well as other patients, healthcare workers and the environment.
Department of Medical Microbiology, University Hospital of Leiden, The Netherlands.
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D000764:Anesthesia, Closed-Circuit / Q000295:instrumentation*; D003428:Cross Infection / Q000635:transmission*; D004866:Equipment Contamination / Q000517:prevention & control*; D004867:Equipment Design; D005374:Filtration; D006761:Hospitals; D006801:Humans; D008954:Models, Biological; D009330:Nebulizers and Vaporizers; D016544:Patient Simulation; D012706:Serratia marcescens