A crude extract of Artocarpus integrifolia contains two lectins with distinct biological activities.
The crude extract derived from seeds of Artocarpus integrifolia (jack fruit) contains two fractions with different biological activities for lymphocytes. One fraction is the D-galactose-binding lectin, jacalin, obtained by affinity purification on a D-galactose agarose column. The other, which is a component of the flow-through fraction (FT), is responsible for the mitogenic activity observed with human PBMC and murine spleen cells. In contrast, jacalin inhibits FT- and ConA-induced proliferative activity of human PMBC and murine spleen cells. This inhibition is not due to toxicity, because: (1) jacalin induces significant levels of IL-3/GM-CSF but not of IL-2 and/or IL-4 in murine spleen cells; (2) jacalin does not affect the capacity of these cells to secrete IL-2 or IL-4 as supernatants obtained from spleen cells sequentially stimulated with jacalin and ConA contain IL-2 and/or IL-4 as well as IL-3/GM-CSF. The ligand for the mitogen contained in the FT fraction is D-mannose as determined by sugar inhibition studies.
Department of Immunology, Ribeirão Preto Medical School, University of São Paulo, Brazil.
I K
IK
de Miranda-Santos
M
M
Delgado
P V
PV
Bonini
M M
MM
Bunn-Moreno
A
A
Campos-Neto
D000818:Animals; D005690:Galactose / Q000378:metabolism; D016179:Granulocyte Colony-Stimulating Factor / Q000378:metabolism; D006801:Humans; D007377:Interleukin-3 / Q000378:metabolism; D037102:Lectins / Q000737:chemistry* / Q000378:metabolism; D008213:Lymphocyte Activation / Q000187:drug effects; D008358:Mannose / Q000031:analogs & derivatives / Q000378:metabolism; D051379:Mice; D010936:Plant Extracts / Q000737:chemistry*; D037121:Plant Lectins; D010944:Plants / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry*
D007377:Interleukin-3; D037102:Lectins; D010936:Plant Extracts; D037121:Plant Lectins; C043696:jacalin; D016179:Granulocyte Colony-Stimulating Factor; D008358:Mannose; D005690:Galactose