Hippocampal neuronal responsiveness to NMDA agonists and antagonists in the adult rat neonatally treated with MK-801.
Long-lasting effects of neonatal interference with N-methyl-D-aspartate (NMDA) receptors were investigated by measuring responses to micro-iontophoretically applied NMDA agonists/antagonist in hippocampal neurons of the adult rat. Rat pups were chronically treated with MK-801 from postnatal day 8 through 19 and tested at postnatal day 70-100. CA1 cell responses to glutamate were not affected by the neonatal treatment. However, a stronger suppression of the NMDA evoked response by the NMDA site antagonist amino-5-phosphonovalerate (APV) was measured, suggesting a long-lasting configurational change of the NMDA receptor. The NMDA evoked responses were equally strong suppressed by MK-801 in both groups, suggesting that channel sites were not affected by this treatment.
Netherlands Institute for Brain Research, Amsterdam.
J A
JA
Gorter
M
M
Veerman
M
M
Mirmiran
D015763:2-Amino-5-phosphonovalerate / Q000494:pharmacology; D000375:Aging / Q000187:drug effects*; D000818:Animals; D000831:Animals, Newborn; D016291:Dizocilpine Maleate / Q000494:pharmacology*; D005260:Female; D005971:Glutamates / Q000494:pharmacology; D018698:Glutamic Acid; D006624:Hippocampus / Q000166:cytology / Q000187:drug effects*; D007478:Iontophoresis; D008297:Male; D009474:Neurons / Q000187:drug effects*; D051381:Rats; D011919:Rats, Inbred Strains; D016194:Receptors, N-Methyl-D-Aspartate / Q000037:antagonists & inhibitors / Q000187:drug effects*
D005971:Glutamates; D016194:Receptors, N-Methyl-D-Aspartate; D018698:Glutamic Acid; D016291:Dizocilpine Maleate; D015763:2-Amino-5-phosphonovalerate