[Characterization of a substance which protects Klebsiella pneumoniae from the bactericidal effect of normal human serum].
Chemical composition of a substance produced by Klebsiella pneumoniae which is accumulated in chemically defined medium was determined. This substance (the protective factor) protects the bacterium from being killed by normal human serum. Protective factor was treated with DNase, lysozyme and an alkaline protease. The two former enzymes did not affect the protective factor. On the other hand, when alkaline protease was used, the protective activity was totally lost. Results showed that the protective factor is a protein.
Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, México D.F.
O
O
Rodas-Suárez
A
A
Martínez-Ramos
D001426:Bacterial Proteins / Q000302:isolation & purification* / Q000494:pharmacology; D001685:Biological Factors / Q000302:isolation & purification* / Q000494:pharmacology; D001770:Blood Bactericidal Activity / Q000187:drug effects*; D002847:Chromatography, Agarose; D017077:Culture Media, Conditioned / Q000737:chemistry; D003851:Deoxyribonucleases / Q000378:metabolism; D010450:Endopeptidases / Q000378:metabolism; D006801:Humans; D007711:Klebsiella pneumoniae / Q000737:chemistry / Q000502:physiology*; D009113:Muramidase / Q000378:metabolism
D001426:Bacterial Proteins; D001685:Biological Factors; D017077:Culture Media, Conditioned; D003851:Deoxyribonucleases; D009113:Muramidase; D010450:Endopeptidases
Caracterización de una sustancia que protege a Klebsiella pneumoniae del efecto bactericida del suero humano normal.