Production of recombinant human interleukin-2 with BHK cells in a hollow fibre and a stirred tank reactor with protein-free medium.
For the production of recombinant human interleukin-2 (IL-2) two different culture processes, a 1-2 liter homogeneous stirred bubble-free aerated system and a dense cell hollow fibre bioreactor were compared. Cultivations were carried out with serum- or protein-free medium formulations. In the stirred culture 0.75 mg IL-2 were produced with 1 l of perfused medium at a maximum cell number of 3 X 10(10). The product yield in the hollow fibre module was only 0.23 mg l-1 at a maximum cell number of 6 X 10(10). In contrast to results with hybridoma or EBV-transformed cell lines, in which hollow fibre bioreactors showed comparable efficiency to perfused stirred tank reactors, the tissue-like cell density is disadvantageous as adherent cells tend to stick together leaving insufficient intercellular space for removal of product.
Ryll
T
T
Gesellschaft für Biotechnologische Forschung, Arbeitsgruppe Zellkulturtechnik, Braunschweig, F.R.G.
Lucki-Lange
M
M
Jäger
V
V
Wagner
R
R
D000818:Animals; D002452:Cell Count; D002478:Cells, Cultured; D006224:Cricetinae; D005285:Fermentation; D005818:Genetic Engineering / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D007376:Interleukin-2 / Q000096:biosynthesis*; D007770:L-Lactate Dehydrogenase / Q000096:biosynthesis; D011994:Recombinant Proteins / Q000096:biosynthesis
D007376:Interleukin-2; D011994:Recombinant Proteins; D007770:L-Lactate Dehydrogenase