Microbial glycolipid production under nitrogen limitation and resting cell conditions.
Rhodococcus erythropolis is able to synthesize an anionic trehalose-2,2',3,4-tetraester during cultivation on n-alkanes. Preconditions for an overproduction are nitrogen limitation, temperature- and pH-shift. The optimum carbon source was technical grade n-C-10, which led to 0.35 g g-1 of glycolipid per n-alkane. Electron microscopical observations showed that n-C-14,15 (technical grade) grown cells contained numerous lipid inclusions in contrast to n-C-10 (technical grade) grown cells. Nocardia corynebacteroides synthesizes a novel pentasaccharide lipid and as size products small amounts of trehalose-corynomycolates. Optimum precursors for overproduction are n-alkanes from n-tetradecane to n-hexadecane with yields in the range of 0.17 g g-1 of glycolipid per carbon source.
Institut für Biochemie und Biotechnologie, Technische Universität, Braunschweig, F.R.G.
J S
JS
Kim
M
M
Powalla
S
S
Lang
F
F
Wagner
H
H
Lünsdorf
V
V
Wray
D001709:Biotechnology; D006017:Glycolipids / Q000096:biosynthesis*; D007399:Interphase; D015394:Molecular Structure; D009584:Nitrogen / Q000494:pharmacology; D009615:Nocardia / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D009844:Oligosaccharides / Q000096:biosynthesis; D012240:Rhodococcus / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D013501:Surface-Active Agents / Q000378:metabolism
D006017:Glycolipids; D009844:Oligosaccharides; D013501:Surface-Active Agents; D009584:Nitrogen