Hepatocyte response to continuous low dose-rate radiation in radioimmunotherapy assessed by micronucleus assay.
The response of hepatocytes to low dose-rate irradiation was examined in mice following the injection of radiolabelled monoclonal antibody. Mice were injected intravenously with an 131I-labelled monoclonal antibody 196-14 which recognizes CA125 antigen, and the effect of continuous low dose-rate irradiation on hepatocytes was assessed using the micronucleus assay. The frequency of micronuclei increased in a dose-dependent fashion, but it was lower than the frequency induced by conventional external X-rays which was determined immediately after the irradiation. A linear quadratic model (micronucleus frequency = aD+bD2+c) showed that the value of b decreased with low dose-rate irradiation from the radiolabelled antibody. It is concluded that the micronucleus assay is useful for the evaluation of the response of hepatocytes to irradiation in radioimmunotherapy.
Sakahara
H
H
Department of Nuclear Medicine, Kyoto University Hospital, Japan.
Ono
K
K
Saga
T
T
Akuta
K
K
Endo
K
K
Konishi
J
J
Abe
M
M
D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism / Q000627:therapeutic use; D005260:Female; D007074:Immunoglobulin G / Q000378:metabolism / Q000627:therapeutic use; D007457:Iodine Radioisotopes / Q000493:pharmacokinetics / Q000627:therapeutic use; D008099:Liver / Q000166:cytology / Q000378:metabolism / Q000528:radiation effects*; D051379:Mice; D008809:Mice, Inbred C3H; D015162:Micronucleus Tests; D016499:Radioimmunotherapy; D011879:Radiotherapy Dosage
D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D007457:Iodine Radioisotopes