L-sorbose does not cause hemolysis in dog erythrocytes with inherited high Na, K-ATPase activity.
1. The hemolytic effect of L-sorbose on canine erythrocytes characterized by inherited high Na, K-ATPase activity and a high potassium concentration (HK RBCs) was compared with that on normal canine erythrocytes (LK RBCs). 2. Dogs having HK RBCs (HK dogs) revealed no clinical and hematological changes after administration of L-sorbose, whereas normal dogs (LK dogs) developed severe hemolytic anemia associated with hemoglobinuria and marked decreases of erythrocyte ATP concentrations. 3. In vitro, L-sorbose induced hemolysis in LK RBCs along with the depression of both ATP and lactate formation in these cells, but not in HK RBCs. The inhibition of glycolysis by L-sorbose in LK RBCs, however, was not observed when glucose-6-phosphate was used as a substrate instead of glucose. 4. These results suggest that the disparity of susceptibility to sorbose-induced hemolysis may be due to the difference in erythrocyte metabolism between HK and LK RBCs, especially the high activity of hexokinase in HK cells, which was 2-fold greater than that in LK RBCs.
Goto
I
I
Department of Veterinary Internal Medicine, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
Shimizu
T
T
Maede
Y
Y
D000255:Adenosine Triphosphate / Q000097:blood; D000818:Animals; D004285:Dogs / Q000097:blood*; D004912:Erythrocytes / Q000187:drug effects* / Q000201:enzymology; D006461:Hemolysis / Q000187:drug effects*; D006801:Humans; D007773:Lactates / Q000096:biosynthesis; D019344:Lactic Acid; D008297:Male; D000254:Sodium-Potassium-Exchanging ATPase / Q000097:blood* / Q000235:genetics; D013013:Sorbose / Q000494:pharmacology*
D007773:Lactates; D019344:Lactic Acid; D000255:Adenosine Triphosphate; D000254:Sodium-Potassium-Exchanging ATPase; D013013:Sorbose