Plasmid stabilization of an Escherichia coli culture through cycling.
The problem of plasmid instability of fermentations that involve plasmid-bearing recombinant organisms is dealt with in this work. Previous theoretical work demonstrated that under certain conditions (where plasmid-bearing species are slower in responding to changes in the fermentation environment than the wild species) the washout of the plasmid-bearing species can be prevented. In the sequel, Weber and San showed that cycling the dilution rate can delay the washout of plasmid-bearing species for a plasmid-bearing Escherichia coli culture. This work shows that it is indeed possible to secure the presence of the plasmid-bearing species at all times through appropriate cycling.
Stephens
M L
ML
Department of Chemical Engineering, University of Florida, Gainesville 32611.
Christensen
C
C
Lyberatos
G
G
D000667:Ampicillin; D001431:Bacteriological Techniques; D004926:Escherichia coli / Q000235:genetics*; D005285:Fermentation; D005818:Genetic Engineering; D010403:Penicillin Resistance / Q000235:genetics; D010957:Plasmids; D013753:Tetracycline Resistance / Q000235:genetics
D000667:Ampicillin