L-tryptophan alleviates fatty liver and modifies hepatic microsomal mixed function oxidase in laying hens.
1. Three experiments were conducted to study the effect of dietary L-tryptophan supplementation (250-1000 ppm) on lipid accumulation, an occurrence of hemorrhages and microsomal mixed function oxidase in the liver of laying hens. 2. Dietary L-tryptophan supplementation resulted in significant decreases in hepatic lipids, in particular triglyceride, and occurrence of hemorrhage in laying hens. 3. Hepatic lipid accumulation by estrogen injection in starved-refed growing chicks decreased as dietary tryptophan content increased. 4. Supplementation of L-tryptophan at 1000 mg/kg diet enhanced alanine aminotransferase activity in the hepatic tissue and at 500 mg/kg diet, increased cytochrome b5, a component of the mixed function oxidase, in the hepatic microsomes. 5. These results demonstrate that L-tryptophan alleviates fatty liver in laying hens and modifies microsomal mixed function oxidase in the liver.
Akiba
Y
Y
Department of Animal Science, Faculty of Agriculture, Tohoku University, Sendai, Japan.
Takahashi
K
K
Horiguchi
M
M
Ohtani
H
H
Saitoh
S
S
Ohkawara
H
H
D000818:Animals; D002645:Chickens; D004032:Diet; D005234:Fatty Liver / Q000517:prevention & control / Q000662:veterinary; D005260:Female; D050356:Lipid Metabolism; D008099:Liver / Q000378:metabolism*; D008862:Microsomes, Liver / Q000201:enzymology*; D006899:Mixed Function Oxygenases / Q000378:metabolism*; D011201:Poultry Diseases / Q000517:prevention & control; D014364:Tryptophan / Q000008:administration & dosage / Q000494:pharmacology*
D014364:Tryptophan; D006899:Mixed Function Oxygenases