Development and inhibition of mouse ear oedema induced with capsaicin.
Oedema was induced in one ear of male mice of the CFLP strain with capsaicin solution (10 microliters/40 micrograms/ear). The development in time and the extent of the oedema were determined by the oedema-disk gravimetric technique. The maximum oedema was attained in less than 1 h, and there was, subsequently, a gradual decrease. The extent of the mouse ear oedema induced in this way and measured after 60 min was inhibited to a statistically significant degree and in a dose-dependent manner by the antihistamine chloropyramine, the antihistamine-antiserotonin cyproheptadine, the non-steroidal anti-inflammatory agent piroxicam, the prostaglandin antagonist di-4-phloretin phosphate, and the lipoxygenase inhibitor nordihydroguairetic acid. The method proved suitable for the detection of oedema and for the biologically quantitative determination of the state of desensitization induced with capsaicin.
Gábor
M
M
Institute of Pharmacodynamics, Albert Szent-Györgyi Medical University, Szeged, Hungary.
Rázga
Z
Z
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000627:therapeutic use; D002211:Capsaicin / Q000037:antagonists & inhibitors / Q000633:toxicity*; D004427:Ear Diseases / Q000139:chemically induced / Q000473:pathology*; D004431:Ear, External; D004487:Edema / Q000139:chemically induced / Q000188:drug therapy / Q000473:pathology*; D006634:Histamine H1 Antagonists / Q000627:therapeutic use; D008297:Male; D051379:Mice
D000894:Anti-Inflammatory Agents, Non-Steroidal; D006634:Histamine H1 Antagonists; D002211:Capsaicin