Ambruticin S production in air-lift and stirred-tank bioreactors.
Using the method of equi-inocular synchronized comparative fermentation (EISCF) the cultivation of Sorangium cellulosum So ce 10 and production of the polyketide antibiotic ambruticin S was compared in stirred-tank and air-lift reactors of different geometry. This method requires that inocula originate from the same pre-culture and cultivation parameters are synchronized to similar values. Similar ambruticin yields were obtained from both reactor systems provided that the concentration of dissolved oxygen (DO) was maintained above a certain value (ca. 40%). For cultivation of S. cellulosum it is the DO level rather than the oxygen transfer rate which presents the proper criterion for scale-up and comparative reactor studies.
Hopf
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Biochemical Engineering Division, GBF-National Research Institute for Biotechnology, Braunschweig, Federal Republic of Germany.
Yonsel
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S
Deckwer
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WD
D000409:Alanine / Q000378:metabolism; D000935:Antifungal Agents / Q000378:metabolism*; D002245:Carbon Dioxide / Q000378:metabolism; D005285:Fermentation; D009161:Mycobacterium / Q000235:genetics; D010100:Oxygen / Q000378:metabolism; D011714:Pyrans / Q000378:metabolism
D000935:Antifungal Agents; D011714:Pyrans; D002245:Carbon Dioxide; D000409:Alanine; D010100:Oxygen; C014964:ambruticin