Comparative analysis of inositol phospholipid metabolism in viral and chemical transformation of mammalian cells.
1. Inositol phospholipid metabolites were measured from virally and chemically transformed cells. 2. Increased levels of PIP and PIP2 were observed from both transformed cell lines as compared with controls. 3. Intracellular levels of IP3 were also increased approximately three folds in BPV-1 infected ID 13 cells and in 3-MC transformed NIH 3T3 cells. 4. The results suggest that phosphorylation of phosphatidylinositols and enhanced generation of IP3 second messenger molecules are the common signal transducing process leading to the cell transformation.
Department of Life Science, Korea Advanced Institute of Science and Technology, Taejon.
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Kim
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Kim
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Choe
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Kang
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Joe
D000818:Animals; D052688:Bovine papillomavirus 1 / Q000502:physiology; D002455:Cell Division; D002461:Cell Line, Transformed; D002471:Cell Transformation, Neoplastic; D002472:Cell Transformation, Viral; D006868:Hydrolysis; D015544:Inositol 1,4,5-Trisphosphate / Q000378:metabolism; D007295:Inositol Phosphates / Q000378:metabolism; D008748:Methylcholanthrene / Q000633:toxicity; D051379:Mice; D010716:Phosphatidylinositols / Q000378:metabolism*; D010766:Phosphorylation; D015398:Signal Transduction
D007295:Inositol Phosphates; D010716:Phosphatidylinositols; D008748:Methylcholanthrene; D015544:Inositol 1,4,5-Trisphosphate