Reactivity of gamma delta T cells induced by the tumour cell line RPMI 8226: functional heterogeneity of clonal populations and role of GroEL heat shock proteins.
The human tumour cell lines RPMI 8226 and Daudi are potent inducers of V gamma 9-expressing T cells. The inducing element of RPMI 8226 has not been defined but evidence suggests that a member of the GroEL heat shock protein (HSP) family (HSP 58) may have a role in the induction by Daudi cells. The present study examined the reactivity patterns of gamma delta T-cell clones generated in response to RPMI 8226 and addressed the possible role of HSP 58 in this process. RPMI 8226 induced a population of V gamma 9 TCR+ cells which were heterogeneous in terms of their cell surface markers, patterns of proliferation and cytotoxic responses. All clones expressed CD3, CD2, CD18 and CD29. They demonstrated variability in expression of CD56, CD8 and HLA-DR. RPMI 8226 stimulated proliferation in purified bulk gamma delta cultures and clones. Daudi was also capable of inducing these cells to proliferate while mycobacterial products were not effective. The clones demonstrated a limited non-MHC-restricted cytotoxicity pattern with some evidence of clonal heterogeneity. Although both Daudi and RPMI 8226 were sensitive to lysis by the clones, cold inhibition experiments indicated differential activity towards these targets. Anti-HSP 58 was inhibitory to gamma delta T-cell induction by RPMI 8226, Daudi and mycobacterial products. However, the anti-HSP 58 antibody appears to bind to the surface of at least six different tumour cell lines with no correlation to their ability to induce gamma delta T cells and the anti-HSP 58 inhibited non-gamma delta responses.
Rheumatic Disease Unit Research Laboratory, University of Manitoba, Winnipeg, Canada.
L K
LK
Selin
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S
Stewart
C
C
Shen
H Q
HQ
Mao
J A
JA
Wilkins
D001426:Bacterial Proteins / Q000276:immunology*; D018834:Chaperonin 60; D002999:Clone Cells; D003602:Cytotoxicity, Immunologic; D006360:Heat-Shock Proteins / Q000276:immunology*; D006801:Humans; D066298:In Vitro Techniques; D007376:Interleukin-2 / Q000494:pharmacology; D008213:Lymphocyte Activation; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000276:immunology*; D011994:Recombinant Proteins; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D014407:Tumor Cells, Cultured / Q000276:immunology*
D001426:Bacterial Proteins; D018834:Chaperonin 60; D006360:Heat-Shock Proteins; D007376:Interleukin-2; D016692:Receptors, Antigen, T-Cell, gamma-delta; D011994:Recombinant Proteins