The expression of presynaptic t-ACPD receptor in rat cerebellum.
The expression of a receptor subtype for one type of excitatory amino acid agonist, t-ACPD, was examined in developing Purkinje cells of cerebellar slices. The t-ACPD-induced responses were compared with those induced by QA in current response, single cell Ca2+ imaging and changes in the miniature currents in the same preparation. It was found that t-ACPD induced a single component of inward current, and an increase in the frequency of miniature currents associated with the presence of external Ca2+, but was ineffective at mobilizing intracellular Ca2+ even in the presence of external Ca2+. The present study suggests the expression of at least two types of metabotropic receptors in the Purkinje cell region, one of which, expressed in the Purkinje cell dendrites, is highly sensitive to QA, but relatively insensitive to t-ACPD, and the other of which is a t-ACPD-sensitive receptor expressed on the presynaptic terminals of the neurons making synapses onto Purkinje cells.
Department of Molecular Neurobiology, School of Human Sciences, Waseda University, Tokorozawa, Japan.
H
H
Takagi
H
H
Takimizu
J
J
de Barry
Y
Y
Kudo
T
T
Yoshioka
D018750:6-Cyano-7-nitroquinoxaline-2,3-dione; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002531:Cerebellum / Q000502:physiology*; D003515:Cycloleucine / Q000031:analogs & derivatives* / Q000494:pharmacology; D003712:Dendrites / Q000187:drug effects / Q000502:physiology / Q000648:ultrastructure; D005071:Evoked Potentials / Q000187:drug effects; D005456:Fluorescent Dyes; D006575:Heterocyclic Compounds, 3-Ring; D066298:In Vitro Techniques; D008564:Membrane Potentials / Q000187:drug effects; D009498:Neurotoxins / Q000494:pharmacology*; D011689:Purkinje Cells / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D011810:Quinoxalines / Q000494:pharmacology; D016318:Quisqualic Acid / Q000494:pharmacology; D051381:Rats; D018094:Receptors, Metabotropic Glutamate; D017981:Receptors, Neurotransmitter / Q000187:drug effects / Q000502:physiology*; D013569:Synapses / Q000502:physiology*
D005456:Fluorescent Dyes; D006575:Heterocyclic Compounds, 3-Ring; D009498:Neurotoxins; D011810:Quinoxalines; D018094:Receptors, Metabotropic Glutamate; D017981:Receptors, Neurotransmitter; C073111:metabotropic quisqualate receptor; D003515:Cycloleucine; C068483:rhod-2; C036361:1-amino-1,3-dicarboxycyclopentane; D018750:6-Cyano-7-nitroquinoxaline-2,3-dione; D016318:Quisqualic Acid; D002118:Calcium