Comparisons of rotavirus VP7-typing monoclonal antibodies by competition binding assay.
Three sets of neutralizing monoclonal antibodies (MAbs) used to type the outer capsid protein VP7 of four group A rotavirus serotypes (1 through 4) were compared in competition immunoassays. Reciprocal competition was observed for each of the VP7 type 2-, 3-, and 4-specific MAbs. The VP7 type 1 MAbs exhibited variable competition patterns with other VP7 type 1 MAbs. MAb RV4:3, which has been used to recognize antigenic variants within VP7 type 1 strains, showed reciprocal competition with the four VP7 type 3 MAbs (RV3:1, YO-1E2, 4F8, and 159) using a VP7 type 3 virus (SA11) as antigen. MAb 2C9, also prepared against VP7 type 1, reacted with VP7 type 3 strains and competed with a VP7 type 3 MAb, 159, using RRV as antigen. Use of the different sets of VP7 type-specific MAbs in the enzyme-linked immunosorbent assay permitted the recognition of six antigenic variants within VP7 types 1, 2, and 3 among specimens whose VP7 type could not be determined previously with only one set of typing MAbs. These results demonstrate differences of typing ability among these VP7-specific MAbs and emphasize the need to improve the sensitivity of typing systems by incorporating panels of MAbs reacting with several neutralizing epitopes.
Raj
P
P
Department of Pediatrics, Baylor College of Medicine, Houston, Texas 77030.
Matson
D O
DO
Coulson
B S
BS
Bishop
R F
RF
Taniguchi
K
K
Urasawa
S
S
Greenberg
H B
HB
Estes
M K
MK
D000911:Antibodies, Monoclonal; D000940:Antigenic Variation; D000956:Antigens, Viral / Q000145:classification; D015373:Bacterial Typing Techniques; D001667:Binding, Competitive; D002213:Capsid / Q000145:classification / Q000276:immunology*; D036022:Capsid Proteins; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D000939:Epitopes; D005069:Evaluation Studies as Topic; D006801:Humans; D009500:Neutralization Tests; D012401:Rotavirus / Q000145:classification / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D036022:Capsid Proteins; D000939:Epitopes; C050779:VP7 protein, Rotavirus