Ethanol-inducible microsomal aniline hydroxylase activity and cytochrome P-450 isozymes in adult hen liver.
1. Cytochrome P-450 was induced in adult hen liver by administering 15% ethanol in drinking water and compared with other inducers such as phenobarbital and beta-naphthoflavone. 2. Aniline was the only substrate whose turnover was induced by ethanol treatment when measured in the presence of 100 microM alpha-naphthoflavone. 3. The inhibitor alpha-naphthoflavone differentiated aniline and p-nitrophenol hydroxylase activities, while p-hydroxyphenyl imidazole and SKF differentiated p-nitrophenol hydroxylase activity between ethanol- and beta-naphthoflavone-induced microsomes. 4. Ethanol treatment also slightly induced some P-450 isozymes related to phenobarbital and beta-naphthoflavone inducers.
Laboratory of Neurotoxicology, Duke University Medical Center, Durham, North Carolina 27710.
R P
RP
Gupta
M B
MB
Abou-Donia
D000815:Aniline Hydroxylase / Q000096:biosynthesis*; D000818:Animals; D001571:Benzoflavones / Q000494:pharmacology; D002384:Catalysis; D002645:Chickens / Q000378:metabolism*; D019392:Cytochrome P-450 CYP2E1; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis / Q000187:drug effects; D004790:Enzyme Induction / Q000502:physiology; D000431:Ethanol / Q000494:pharmacology*; D005260:Female; D007527:Isoenzymes / Q000096:biosynthesis; D007700:Kinetics; D008797:Metyrapone / Q000494:pharmacology; D008862:Microsomes, Liver / Q000187:drug effects* / Q000201:enzymology; D006899:Mixed Function Oxygenases / Q000096:biosynthesis* / Q000187:drug effects; D010634:Phenobarbital / Q000494:pharmacology; D019324:beta-Naphthoflavone
D001571:Benzoflavones; D007527:Isoenzymes; D000431:Ethanol; D019324:beta-Naphthoflavone; D003577:Cytochrome P-450 Enzyme System; D006899:Mixed Function Oxygenases; D019392:Cytochrome P-450 CYP2E1; D000815:Aniline Hydroxylase; D010634:Phenobarbital; D008797:Metyrapone