A search for Pneumocystis carinii in post-mortem lungs by DNA amplification.
DNA amplification of specific sequences and subsequent oligonucleotide hybridization were used to search for Pneumocystis carinii in post-mortem lung samplings from non-immunosuppressed individuals ranging from 15 to 70 years of age. No P. carinii-specific DNA was detected in 45 DNA amplification reactions from 15 lungs.
Department of Paediatrics, John Radcliffe Hospital, Oxford, U.K.
S E
SE
Peters
A E
AE
Wakefield
K
K
Sinclair
P R
PR
Millard
J M
JM
Hopkin
D000293:Adolescent; D000328:Adult; D000368:Aged; D004587:Electrophoresis, Agar Gel; D004996:Ethidium; D006801:Humans; D008168:Lung / Q000382:microbiology*; D008875:Middle Aged; D015345:Oligonucleotide Probes; D011010:Pneumocystis / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D013194:Staining and Labeling
D015345:Oligonucleotide Probes; D004996:Ethidium