Effects of urogastrone-epidermal growth factor and age at administration on five enzymes in the small intestine of suckling rats.
Suckling rats were given urogastrone-epidermal growth factor (EGF: 1,000 micrograms/kg body weight) or vehicle by gavage at one of three stages of development: 8 to 10, 11 to 13 or 14 to 16 days of age. Intubation was carried out at 8-hourly intervals over these periods. Fourteen to 16 h after the last intubation the rats were killed; that is, at 11, 14 and 17 days respectively. Samples of proximal and distal small intestine (SI) were taken for enzyme analysis. Five enzymes were assayed; sucrase, lactase, gamma-glutamyl transferase, alkaline phosphatase and neutral amino-peptidase, and their activities expressed per g protein. Treatment with EGF had no effect on body weight or on the length of the small intestine at any age. The nature of the effects on enzyme activities depended on the specific enzyme concerned, the site within the small intestine and the timing of the treatment. Lactase was increased by EGF at both sites only on day 14, whereas gamma-glutamyl transferase was increased in proximal samples at 11 and 14 days, and in distal samples at 17 days. Nor was the outcome always to increase activity. On day 11 alkaline phosphatase was increased in proximal SI, but decreased in distal SI; and so too was aminopeptidase N decreased in distal SI at 11 days. Sucrase showed no response at all. The pattern is complex. Certainly it does not indicate accelerated functional maturation.
Department of Child Health, St Mary's Hospital, Manchester.
F M
FM
Bradbury
J L
JL
Smart
S W
SW
D'Souza
R W
RW
Lobley
P C
PC
Burrows
P W
PW
Pemberton
D000367:Age Factors; D000469:Alkaline Phosphatase / Q000378:metabolism*; D000626:Aminopeptidases / Q000378:metabolism*; D000818:Animals; D000833:Animals, Suckling / Q000378:metabolism; D018826:CD13 Antigens; D004789:Enzyme Activation / Q000187:drug effects; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D007421:Intestine, Small / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development; D043322:Lactase; D051381:Rats; D013393:Sucrase / Q000378:metabolism; D001616:beta-Galactosidase / Q000378:metabolism; D005723:gamma-Glutamyltransferase / Q000378:metabolism*
D004815:Epidermal Growth Factor; D005723:gamma-Glutamyltransferase; D000469:Alkaline Phosphatase; D043322:Lactase; D001616:beta-Galactosidase; D013393:Sucrase; D000626:Aminopeptidases; D018826:CD13 Antigens