Failure to detect cytomegalovirus DNA in pancreas in type 2 diabetes.
We have attempted to confirm the finding of cytomegalovirus (CMV) nucleic acid sequences in pancreas sections from patients with type 2 diabetes in a large population since this finding has major implications for the pathogenesis of the disorder. A highly sensitive nested polymerase chain reaction method was developed to detect the immediate-early CMV gene in DNA extracted from wax-embedded tissue sections. We could not confirm the previous findings; CMV DNA was not detected in pancreas sections from 43 type 2 diabetic patients or from 38 non-diabetic age-matched subjects, although the method detected CMV DNA, even in very low concentrations, in positive controls.
Nuffield Department of Medicine, Radcliffe Infirmary, Oxford, UK.
A T
AT
Hattersley
Y M
YM
Lo
S J
SJ
Read
R P
RP
Eglin
J S
JS
Wainscoat
A
A
Clark
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001483:Base Sequence; D001609:Beta-Globulins / Q000235:genetics; D003587:Cytomegalovirus / Q000235:genetics / Q000302:isolation & purification*; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification*; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics / Q000382:microbiology*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D010179:Pancreas / Q000382:microbiology*; D016133:Polymerase Chain Reaction
D001609:Beta-Globulins; D004279:DNA, Viral