Determination of alanine aminotransferase in human serum in an open-closed flow injection configuration.
Open-closed flow injection systems allow the development of methods based on multidetection by using conventional detectors. The methods proposed here for the determination of alanine aminotransferase (ALT) utilize a photometric detector included in the closed circuit, which allows one to perform fixed-time and reaction-rate measurements whose sensitivity can be increased by using the sum of the analytical signals. The methods, with determination ranges between 1 and 500 U l-1 of ALT, feature sampling frequencies up to 60 h-1. Their application to serum samples provided excellent results, with recoveries between 95 and 106% and averaging at 99.9%.
Department of Analytical Chemistry, Faculty of Sciences, University of Córdoba, Spain.
J M
JM
Fernández-Romero
M D
MD
Luque de Castro
M
M
Valcárcel
D000410:Alanine Transaminase / Q000097:blood*; D002138:Calibration; D006801:Humans; D008722:Methods; D010783:Photometry
D000410:Alanine Transaminase