Oral absorption of biologically active insulin in common carp (Cyprinus carpio L.).
1. Bovine insulin dissolved in 0.05 M deoxycholic acid was absorbed through oral intubation in fish weighing 200-300 g. 2. The peak of absorption appeared in all fish, 30-45 min after intubation and was followed by a gradual decrease. The extent of absorption was extremely variable, with up to a 20-fold difference between individuals. No detectable insulin was found in fish intubated with vehicle. 3. The absorbed hormone retained 17-88% of its lipogenic bioactivity in vitro. The absorbed insulin lowered the levels of several amino acids in the intubated fish, indirectly indicating that its bioactivity was also retained in vivo.
Department of Biochemistry and Human Nutrition, Faculty of Agriculture, Hebrew University of Jerusalem, Rehovot, Israel.
Y
Y
Hertz
Y
Y
Shechter
Z
Z
Madar
A
A
Gertler
D000042:Absorption; D000284:Administration, Oral; D000596:Amino Acids / Q000097:blood; D000818:Animals; D002347:Carps; D007328:Insulin / Q000097:blood / Q000493:pharmacokinetics* / Q000502:physiology; D009055:Mouth / Q000378:metabolism
D000596:Amino Acids; D007328:Insulin