Optimized centrifugation for rapid elution of DNA from agarose gels.
A simple method has been developed for the isolation of DNA from agarose gels. Centrifugation in a low-cost device for 45 s at low speed provides a high yield of DNA suitable for further manipulation by restriction enzymes, T4 DNA ligase, Taq polymerase, Klenow fragment, and T4 polynucleotide kinase, and also for sequencing. In contrast, centrifugation for greater than 1 min leads to coelution of substances that inhibit DNA ligase.
Department of Surgery, St. George's Hospital Medical School, London, UK.
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Austen
D015139:Blotting, Southern; D002498:Centrifugation / Q000295:instrumentation / Q000379:methods; D004247:DNA / Q000302:isolation & purification*; D004274:DNA, Recombinant / Q000302:isolation & purification; D004587:Electrophoresis, Agar Gel / Q000379:methods*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length
D004274:DNA, Recombinant; D004247:DNA