Chemically defined medium for the production of biologically active substances of CHO cells.
A recombinant CHO cell line (GT19) secreting a high level of human growth hormone (hGH) was constructed with amplification of the introduced hGH gene. The cells grew well in the alpha MEM medium supplemented with 5% dialyzed fetal calf serum (dFCS), but not with less than 1% dFCS. Therefore we examined various medium components and obtained an improved medium which supported cell growth at low serum concentrations. The production of hGH by the cells was also enhanced in this medium.
Central Research Laboratory, Nippon Suisan Kaisha, Ltd., Tokyo, Japan.
J
J
Hata
T
T
Tamura
S
S
Yokoshima
S
S
Yamashita
S
S
Kabeno
K
K
Matsumoto
K
K
Onodera
D000818:Animals; D016466:CHO Cells; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D006224:Cricetinae; D003470:Culture Media; D016895:Culture Media, Serum-Free; D046508:Culture Techniques / Q000379:methods; D013006:Growth Hormone / Q000096:biosynthesis* / Q000235:genetics / Q000494:pharmacology; D006801:Humans; D010957:Plasmids; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000494:pharmacology; D015183:Restriction Mapping; D014162:Transfection
D003470:Culture Media; D016895:Culture Media, Serum-Free; D011994:Recombinant Proteins; D013006:Growth Hormone