Transport of acidic amino acids in Candida albicans.
In Candida albicans ATCC 10261, two kinetically different amino acid transport systems with a high (S1) and a low (S2) affinity for aspartic acid (asp) and glutamic acid (glu) were identified. The S1 for the two acidic amino acids was characterized by low Kt values while Kt values of S2 were 30 to 40 times higher. Based on competitive studies of both systems, S1 was found to be specific and common to both asp and glu while S2 was relatively less specific. The S1 and S2 systems were also different in their sensitivity to respiratory inhibitors, mercurials and a K+ channel blocker. Both systems, however, showed maximum transport rates during the mid-exponential growth phase.
School of Life Sciences, Jawaharal Nehru University, New Delhi, India.
P
P
Gupta
S K
SK
Mahanty
S
S
Ansari
R
R
Prasad
D001224:Aspartic Acid / Q000378:metabolism*; D001667:Binding, Competitive; D001692:Biological Transport / Q000187:drug effects; D002176:Candida albicans / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone / Q000494:pharmacology; D003513:Cycloheximide / Q000494:pharmacology; D005656:Fungal Proteins / Q000096:biosynthesis / Q000187:drug effects; D005971:Glutamates / Q000378:metabolism*; D018698:Glutamic Acid; D006863:Hydrogen-Ion Concentration; D007700:Kinetics
D005656:Fungal Proteins; D005971:Glutamates; D001224:Aspartic Acid; D018698:Glutamic Acid; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone; D003513:Cycloheximide