A novel sulfatase from Pseudomonas testosteroni hydrolyzing lithocholic acid sulfate.
Pseudomonas testosteroni ATCC 11996 was found to produce a novel bile acid sulfate sulfatase that hydrolyzes the sulfate ester bond in lithocholic acid sulfate (LCA-S). The enzyme synthesis was induced by several kinds of bile acids including LCA-S. Mn2+ functioned as an essential component for the enzyme synthesis and SO4(2-) suppressed it. This sulfatase hydrolyzes LCA-S to isolithocholic acid and sulfuric acid with inversion of alpha- to beta-configuration of the hydroxyl group at the third position of lithocholic acid.
Tazuke
Y
Y
Kyoto Research Laboratories, Marukin Shoyu Co., Japan.
Matsuda
K
K
Okada
S
S
Tsukada
Y
Y
D002849:Chromatography, Gas; D003470:Culture Media; D004790:Enzyme Induction; D006868:Hydrolysis; D008095:Lithocholic Acid / Q000031:analogs & derivatives* / Q000378:metabolism; D008345:Manganese / Q000494:pharmacology; D011549:Pseudomonas / Q000201:enzymology*; D013429:Sulfatases / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism*; D013431:Sulfates / Q000494:pharmacology; D013464:Sulfuric Acids / Q000378:metabolism
D003470:Culture Media; D013431:Sulfates; D013464:Sulfuric Acids; C014202:sulfolithocholic acid; D008345:Manganese; D008095:Lithocholic Acid; D013429:Sulfatases; C033158:sulfuric acid