L1 substrate enhances outgrowth of tyrosine hydroxylase-immunoreactive neurites in mesencephalic cell culture.
We have evaluated neurite outgrowth from mesencephalic tyrosine hydroxylase-positive neurons grown in vitro on different substrates. Cultures of ventral mesencephalon from rat embryos (E13) were plated on plastic dishes coated with the following substrates: L1, L2/HNK-1 "residual" (mainly J1/160 but also tenascin), MAG antigens from mouse brains, laminin, fibronectin, poly-L-lysine, RGD peptide, and plastic alone. After 3, 4, and 6 days in vitro, the cultures were stained using an antibody against tyrosine hydroxylase (TH), and the length of TH-positive neurites was measured by computer-assisted image analysis in a double-blind fashion. L1 antigen had a significant positive effect on neurite outgrowth compared to the other substrates studied. Laminin and fibronectin were also favorable substrates. In cultures treated with cytosine arabinoside to prevent mitoses and glial proliferation, the positive effect of L1 was abolished, but laminin still had a stimulatory effect. These data indicate that L1 may be indirectly involved in differentiation or axonal elongation of substantia nigra dopaminergic neurons and suggest a complex effect involving both neurons and glia on dopaminergic neurite development.
Preclinical Neurosciences Section, NIMH Neuroscience Center, St. Elizabeths, Washington, D.C. 20032.
M
M
Poltorak
K
K
Shimoda
W J
WJ
Freed
D000818:Animals; D015816:Cell Adhesion Molecules, Neuronal; D002478:Cells, Cultured; D004622:Embryo, Mammalian; D005353:Fibronectins; D007150:Immunohistochemistry; D007797:Laminin; D039841:Leukocyte L1 Antigen Complex; D008636:Mesencephalon / Q000201:enzymology / Q000502:physiology*; D016501:Neurites / Q000201:enzymology / Q000502:physiology* / Q000648:ultrastructure; D009474:Neurons / Q000201:enzymology / Q000502:physiology*; D051381:Rats; D011919:Rats, Inbred Strains; D013379:Substrate Specificity; D014446:Tyrosine 3-Monooxygenase / Q000032:analysis*
D015816:Cell Adhesion Molecules, Neuronal; D005353:Fibronectins; D007797:Laminin; D039841:Leukocyte L1 Antigen Complex; D014446:Tyrosine 3-Monooxygenase