Inhibition of prolyl endopeptidase by synthetic peptide fragments of human beta-casein.
It has been suggested that peptide inhibitors of prolyl endopeptidase (PEP) may act as anti-amnestic agents. In the hope of finding PEP inhibitors in milk proteins, we synthesized a total of 37 human beta-casein peptide fragments containing proline residues. It was found that the peptides with PEP inhibition activity in vitro were located in the region of amino acid residues 49-59 of human beta-casein. The most potent inhibitor was Ile-Tyr-Pro-Phe-Val-Glu-Pro-Ile (IC50 = 8 microM).
Central Research Laboratories, Ajinomoto Co., Inc., Kawasaki, Japan.
M
M
Asano
N
N
Nio
Y
Y
Ariyoshi
D000595:Amino Acid Sequence; D002364:Caseins / Q000737:chemistry / Q000494:pharmacology*; D010450:Endopeptidases / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000494:pharmacology*; D000085822:Prolyl Oligopeptidases; D012697:Serine Endopeptidases; D015842:Serine Proteinase Inhibitors / Q000737:chemistry / Q000494:pharmacology*
D002364:Caseins; D010446:Peptide Fragments; D015842:Serine Proteinase Inhibitors; D010450:Endopeptidases; D012697:Serine Endopeptidases; C000705287:PREPL protein, human; D000085822:Prolyl Oligopeptidases