Antibiotics from basidiomycetes. XLI. Clavicoronic acid, a novel inhibitor of reverse transcriptases from Clavicorona pyxidata (Pers. ex Fr.) Doty.
A novel inhibitor of RNA-directed DNA-polymerases was isolated from fermentations of Clavicorona pyxidata. Its structure was elucidated by spectroscopic methods. Clavicoronic acid (1) is a noncompetitive inhibitor of avian myeloblastosis virus (Ki 130 microM) and Moloney murine leukemia virus (Ki 68 microM) reverse transcriptases. In permeabilized cells and isolated nucleic DNA- and RNA-synthesis are not affected. Clavicoronic acid markedly inhibits the multiplication of vesicular stomatitis virus in baby hamster kidney cells by interfering with this virus's RNA-directed RNA-polymerase. 1 exhibits no cytotoxic and very weak antimicrobial activities.
Erkel
G
G
LB Biotechnologie der Universität, Kaiserslautern, Germany.
Anke
T
T
Gimenez
A
A
Steglich
W
W
D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D009189:Avian Myeloblastosis Virus / Q000187:drug effects / Q000201:enzymology; D001487:Basidiomycota / Q000378:metabolism*; D002460:Cell Line; D005285:Fermentation; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008968:Molecular Conformation; D015394:Molecular Structure; D008979:Moloney murine leukemia virus / Q000187:drug effects / Q000201:enzymology; D012190:Retroviridae / Q000187:drug effects* / Q000201:enzymology; D018894:Reverse Transcriptase Inhibitors; D012717:Sesquiterpenes / Q000737:chemistry / Q000494:pharmacology*; D014721:Vesicular stomatitis Indiana virus / Q000187:drug effects* / Q000201:enzymology
D000998:Antiviral Agents; D018894:Reverse Transcriptase Inhibitors; D012717:Sesquiterpenes; C073556:clavicoronic acid