The identity of Anisakis type II larvae with Anisakis physeteris confirmed by restriction fragment length polymorphism analysis of genomic DNA.
The identity of Anisakis type II larvae with adult A. physeteris was confirmed by comparison of restriction fragment length polymorphisms (RFLPs) of 25S ribosomal DNA (rDNA). Patterns of RFLPs in larvae were almost identical with those in adult worms. Directly labelled 25S rDNA might serve as an appropriate probe with highly specific activity for examining RFLPs of larvae and adult worms.
Department of Parasitology, Shinshu University School of Medicine, Nagano Prefecture, Japan.
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Matsuura
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Sugane
D000818:Animals; D017163:Anisakis / Q000235:genetics / Q000302:isolation & purification*; D015139:Blotting, Southern; D004275:DNA, Ribosomal / Q000032:analysis*; D007814:Larva / Q000235:genetics; D009693:Nucleic Acid Hybridization; D012150:Polymorphism, Restriction Fragment Length; D012335:RNA, Ribosomal / Q000235:genetics*; D015183:Restriction Mapping
D004275:DNA, Ribosomal; D012335:RNA, Ribosomal; C065039:RNA, ribosomal, 25S