Sequencing and population analysis of four novel HLA-DPA1 alleles.
We determined the base sequences of the HLA-DPA1 gene from four B-lymphoblastoid cell lines (CB6B, LKT3, AMAI, and T7526) that showed distinct electrophoretic patterns of single-stranded polymerase chain reaction products of the HLA-DPA1 gene. The novel HLA-DPA1 alleles of CB6B, LKT3, AMAI, and T7526 were designated DPA1*02021, DPA1*02022, DPA1*0301, and DPA1*0401, respectively. Although there was only one base substitution between DPA1*02021 and DPA1*02022, the single-strand conformation polymorphism of these two alleles was clearly demonstrated by electrophoresis in a nondenaturing polyacrylamide gel containing 10% glycerol. In addition, we genotyped for the HLA-DPA1 gene of healthy unrelated Oriental individuals--i.e., 227 Japanese, 88 Papua New Guineans, and 41 Buyi-Chinese--to demonstrate the ethnic distribution of the HLA-DPA1 alleles.
Harada
H
H
Department of Genetic, Kyushu University, Fukuoka, Japan.
Kimura
A
A
Dong
R P
RP
Xu
X P
XP
Bhatia
K
K
Sasazuki
T
T
D000483:Alleles; D000595:Amino Acid Sequence; D044466:Asian People; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D005787:Gene Frequency; D005802:Genes, MHC Class II; D006682:HLA-DP Antigens / Q000235:genetics*; D059867:HLA-DP alpha-Chains; D006801:Humans; D015151:Immunoblotting; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D044465:White People
D006682:HLA-DP Antigens; D059867:HLA-DP alpha-Chains; C071172:HLA-DPA1 antigen