Lactate dehydrogenase (LDH) activity of the cultured eukaryotic cells as marker of the number of dead cells in the medium [corrected].
One significant problem in monitoring a culture's evolution is to assess change in cell viability. We have demonstrated that LDH release could be a good indicator of cellular damage of many cell lines, especially during shear stress or sonication. Moreover, we have found a significant correlation between the number of dead cells, determined by Trypan Blue staining, and LDH activity measurements in the supernatant of hybridoma strains, whatever the culture conditions. We have also shown that when viability is still near 100% no LDH is released even at high cell concentrations. Therefore, LDH should serve as a potential marker of cell injury and death.
Legrand
C
C
Laboratoire de Biochimie Médicale, Faculté de Médecine et IBN, Nancy, France.
Bour
J M
JM
Jacob
C
C
Capiaumont
J
J
Martial
A
A
Marc
A
A
Wudtke
M
M
Kretzmer
G
G
Demangel
C
C
Duval
D
D
D000818:Animals; D016923:Cell Death; D002478:Cells, Cultured; D006224:Cricetinae; D003470:Culture Media; D005260:Female; D007770:L-Lactate Dehydrogenase / Q000032:analysis*; D051379:Mice; D051381:Rats
D003470:Culture Media; D007770:L-Lactate Dehydrogenase