Detection of a new polymorphism of the human prothrombin (F2) gene by combination of PASA and mutated primer-mediated PCR-RFLP.
A new polymorphism of the human prothrombin (F2) gene was detected by a combination of polymerase chain reaction (PCR) amplification of specific alleles (PASA) and mutated primer-mediated PCR restriction fragment length polymorphism (PCR-RFLP). The method is simple and useful for detecting polymorphisms and mutations. The new polymorphism of C1 and C2 examined by this method is highly heterozygous and serves as a good human DNA marker.
Otsuka Department of Clinical and Molecular Nutrition, School of Medicine, University of Tokushima, Japan.
H
H
Iwahana
N
N
Mizusawa
K
K
Yoshimoto
M
M
Itakura
D001483:Base Sequence; D004277:DNA, Single-Stranded; D006579:Heterozygote; D006801:Humans; D008969:Molecular Sequence Data; D009154:Mutation; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D011516:Prothrombin / Q000235:genetics*
D004277:DNA, Single-Stranded; D011516:Prothrombin