The apical sorting signal on human aminopeptidase N is not located in the stalk but in the catalytic head group.
Human aminopeptidase N carries an apical sorting signal on its ectodomain necessary for its correct transport to the apical membrane in Madin-Darby canine kidney cells. To determine whether the apical sorting signal is localized in the serine/threonine rich stalk or in the catalytic head group, anchor/stalk-minus aminopeptidase N, consisting of the hemagglutinin signal peptide and the catalytic head group of human aminopeptidase N, was expressed in MDCK cells. Anchor/stalk-minus aminopeptidase N was secreted mainly to the apical side. The catalytic head group of human aminopeptidase N thus carries an apical sorting signal.
Department of Biochemistry C, Panum Institute, University of Copenhagen, Denmark.
L K
LK
Vogel
O
O
Norén
H
H
Sjöström
D000595:Amino Acid Sequence; D000626:Aminopeptidases / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D015153:Blotting, Western; D018826:CD13 Antigens; D002384:Catalysis; D002478:Cells, Cultured; D004247:DNA; D004285:Dogs; D006801:Humans; D008969:Molecular Sequence Data; D011233:Precipitin Tests
D004247:DNA; D000626:Aminopeptidases; D018826:CD13 Antigens