Diminished capacity to release metabolites of nitric oxide synthase in macrophages loaded with oxidized low-density lipoproteins.
Activation of J774-macrophages with lipopolysaccharide (LPS) or LPS and recombinant interferon-gamma (IFN-gamma) induced nitric oxide (NO) synthase activity, as measured by the production of nitrite and citrulline. NO synthase activity was suppressed by loading the cells with oxidatively modified low-density lipoprotein (ox-LDL) but not with acetylated LDL (ac-LDL), although the intracellular lipid accumulation was comparable. This suggests that the extent of activation of lipid-loaded macrophages may be influenced by the type of lipid.
Jorens
P G
PG
Division of Pharmacology, University of Antwerp (UIA), Wilrijk, Belgium.
Rosseneu
M
M
Devreese
A M
AM
Bult
H
H
Marescau
B
B
Herman
A G
AG
D000594:Amino Acid Oxidoreductases / Q000378:metabolism*; D000818:Animals; D002460:Cell Line; D002784:Cholesterol / Q000032:analysis; D002956:Citrulline / Q000378:metabolism*; D005487:Foam Cells / Q000737:chemistry; D007371:Interferon-gamma / Q000494:pharmacology; D008070:Lipopolysaccharides; D008077:Lipoproteins, LDL / Q000494:pharmacology*; D008264:Macrophages / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D019001:Nitric Oxide Synthase; D009573:Nitrites / Q000378:metabolism*; D011994:Recombinant Proteins
D008070:Lipopolysaccharides; D008077:Lipoproteins, LDL; D009573:Nitrites; D011994:Recombinant Proteins; D002956:Citrulline; D007371:Interferon-gamma; D002784:Cholesterol; D019001:Nitric Oxide Synthase; D000594:Amino Acid Oxidoreductases