Antigen-specific stimulation of T cell extracellular acidification by MHC class II-peptide complexes.
A specific T cell response to a preformed complex of detergent-solubilized MHC class II molecule and cognate antigenic peptide was observed by monitoring the extracellular acidification. An increase in this rate was observed when the resting 4R3.9 T cell clone specific for the peptide fragment MBP(1-14) of myelin basic protein was exposed to preformed detergent-solubilized IAk-MBP(1-14)A4 complexes. MBP peptide alone, IAk alone, or complexes of IAs-proteolipid protein(139-151) and IAd-OVA(323-339), did not cause significant increases in the acidification rates of the MBP(1-14)-restricted 4R3.9 T cell clone. In addition, BW 5147 T lymphoma cells, which lack TCR, did not show any increase in rate when exposed to IAk-MBP(1-14)A4 complexes. Similar increases in acidification rate were observed in the presence of IL-2, anti-CD3 and anti-TCR antibodies. The enhanced acidification responses were blocked by genistein, a tyrosine kinase inhibitor.
Nag
B
B
Anergen Incorporation, Redwood City, CA 94063.
Wada
H G
HG
Fok
K S
KS
Green
D J
DJ
Sharma
S D
SD
Clark
B R
BR
Parce
J W
JW
McConnell
H M
HM
D000143:Acids / Q000378:metabolism; D000595:Amino Acid Sequence; D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology; D017252:CD3 Complex; D002478:Cells, Cultured; D019833:Genistein; D000949:Histocompatibility Antigens Class II / Q000276:immunology*; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology; D007529:Isoflavones / Q000494:pharmacology; D008213:Lymphocyte Activation; D008969:Molecular Sequence Data; D004676:Myelin Basic Protein / Q000276:immunology; D010047:Ovalbumin / Q000276:immunology; D010446:Peptide Fragments / Q000276:immunology*; D011948:Receptors, Antigen, T-Cell / Q000276:immunology; D013601:T-Lymphocytes / Q000378:metabolism*
D000143:Acids; D000945:Antigens, Differentiation, T-Lymphocyte; D017252:CD3 Complex; D000949:Histocompatibility Antigens Class II; D007376:Interleukin-2; D007529:Isoflavones; D004676:Myelin Basic Protein; D010446:Peptide Fragments; D011948:Receptors, Antigen, T-Cell; D010047:Ovalbumin; D019833:Genistein