FAB-MS/MS spectrometry in determining the primary structure of gamma-glutamyl-containing peptides.
Positive fast atom bombardment tandem mass spectrometry (FAB-MS/MS) was applied for peptide sequencing, particularly for determining the gamma glutamyl linkage involved in metal-binding peptides such as Cadystin (gamma EC)3G = Cadystin A and Cadystin (gamma EC)2G = Cadystin B (MW 771 and 539, respectively). The fragmentation patterns between the natural gamma glutamyl peptide and the synthetic alpha glutamyl one were clearly distinguishable. FAB-MS/MS was proved to be a good method for determining these peptides, since it needed no chemical degradation and only a small amount of the peptide was needed for determination.
Laboratory of Organic Chemistry, School of Agriculture, Nagoya University, Japan.
M
M
Isobe
D
D
Uyakul
K L
KL
Liu
T
T
Goto
D000595:Amino Acid Sequence; D002352:Carrier Proteins / Q000737:chemistry; D005656:Fungal Proteins / Q000737:chemistry; D047908:Intracellular Signaling Peptides and Proteins; D008969:Molecular Sequence Data; D015394:Molecular Structure; D010455:Peptides / Q000737:chemistry*; D029702:Schizosaccharomyces pombe Proteins; D016339:Spectrometry, Mass, Fast Atom Bombardment / Q000379:methods
D002352:Carrier Proteins; D005656:Fungal Proteins; D047908:Intracellular Signaling Peptides and Proteins; D010455:Peptides; D029702:Schizosaccharomyces pombe Proteins; C055088:cadystin protein, S pombe