Mechanism of MHC class I downregulation in HIV infected cells.
HIV infection of CD4+ peripheral blood lymphocytes leads to a loss of MHC class I molecules on the surface of the infected cells as detectable by monoclonal antibody staining and flow cytometry. Incubation of the infected cells at 26 degrees C or treatment at 37 degrees C with peptides leads to upregulation of MHC class I to levels equal to those found on uninfected cells cultured under the same conditions. The data suggest that, after HIV infection, the mechanisms responsible for peptide generation, peptide transport and thus stable association between peptides and MHC class I molecules are severely affected.
Institute of Virology and Immunobiology, University of Würzburg, Germany.
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Kerkau
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Gernert
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Kneitz
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Schimpl
D015496:CD4-Positive T-Lymphocytes / Q000378:metabolism / Q000382:microbiology*; D015536:Down-Regulation; D005434:Flow Cytometry; D015683:Gene Products, gag / Q000378:metabolism; D015488:HIV Antigens / Q000378:metabolism; D015497:HIV-1 / Q000378:metabolism*; D015395:Histocompatibility Antigens Class I / Q000378:metabolism*; D014764:Viral Proteins; D001613:beta 2-Microglobulin / Q000378:metabolism; D054301:gag Gene Products, Human Immunodeficiency Virus
D015683:Gene Products, gag; D015488:HIV Antigens; D015395:Histocompatibility Antigens Class I; D014764:Viral Proteins; D001613:beta 2-Microglobulin; D054301:gag Gene Products, Human Immunodeficiency Virus; C054858:p17 protein, Human Immunodeficiency Virus Type 1