Recoverin, a novel calcium-binding protein from vertebrate photoreceptors.
Photoreceptor guanylyl cyclase activity is modulated by an endogenous calcium-binding protein called recoverin. A modified isolation procedure for recoverin using gel-filtration chromatography instead of a heat denaturation step is presented. The elution volume of recoverin corresponds to a monomer. Recoverin exhibits a calcium-dependent mobility shift in a native gel electrophoresis. Isoelectric focusing revealed a pI of 5.25. No subspecies of recoverin were detected.
Institut für Biologische Informationsverarbeitung, Forschungszentrum Jülich, Germany.
H G
HG
Lambrecht
K W
KW
Koch
D000818:Animals; D000906:Antibodies; D000951:Antigens, Neoplasm / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification*; D002135:Calcium-Binding Proteins / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification*; D002417:Cattle; D002850:Chromatography, Gel; D004789:Enzyme Activation; D005136:Eye Proteins; D006162:Guanylate Cyclase / Q000378:metabolism*; D051597:Hippocalcin; D008074:Lipoproteins; D009419:Nerve Tissue Proteins; D010786:Photoreceptor Cells / Q000378:metabolism*; D051603:Recoverin; D012243:Rhodopsin / Q000032:analysis
D000906:Antibodies; D000951:Antigens, Neoplasm; D002135:Calcium-Binding Proteins; D005136:Eye Proteins; D008074:Lipoproteins; D009419:Nerve Tissue Proteins; D051603:Recoverin; D051597:Hippocalcin; D012243:Rhodopsin; D006162:Guanylate Cyclase