Beijerinckia indica var. penicillanicum penicillin V acylase: enhanced enzyme production by catabolite repression-resistant mutant and effect of solvents on enzyme activity.
Beijerinckia indica var. penicillanicum mutant UREMS-5, producing 168% more penicillin V acylase, was obtained by successive treatment with UV, gamma-irradiation and ethylmethane sulfonate. Penicillin V acylase production by the mutant strain was resistant to catabolite repression by glucose. Incorporation of glucose, sodium glutamate and vegetable oils in the medium enhanced enzyme production. The maximum specific production of penicillin V acylase was 244 IU/g dry weight of cells. Effect of solvents on hydrolysis of penicillin V by soluble penicillin V acylase and whole cells was studied. Methylene chloride, chloroform and carbon tetrachloride significantly stimulated the rate of penicillin V hydrolysis by whole cells.
Research and Development, Hindustan Antibiotics Ltd., Pimpri, Pune, India.
S S
SS
Ambedkar
B S
BS
Deshpande
V K
VK
Sudhakaran
J G
JG
Shewale
D002251:Carbon Tetrachloride / Q000494:pharmacology; D002463:Cell Membrane Permeability / Q000187:drug effects; D002725:Chloroform / Q000494:pharmacology; D003470:Culture Media; D005020:Ethyl Methanesulfonate / Q000494:pharmacology; D005285:Fermentation; D005720:Gamma Rays; D005947:Glucose / Q000378:metabolism; D006868:Hydrolysis; D008752:Methylene Chloride / Q000494:pharmacology; D009154:Mutation; D010399:Penicillin Amidase / Q000096:biosynthesis*; D010404:Penicillin V / Q000378:metabolism; D010938:Plant Oils / Q000378:metabolism; D011548:Pseudomonadaceae / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics / Q000528:radiation effects; D012970:Sodium Glutamate / Q000378:metabolism; D012997:Solvents / Q000494:pharmacology*; D014466:Ultraviolet Rays
D003470:Culture Media; D010938:Plant Oils; D012997:Solvents; D008752:Methylene Chloride; D002725:Chloroform; D005020:Ethyl Methanesulfonate; D002251:Carbon Tetrachloride; D010399:Penicillin Amidase; D005947:Glucose; D012970:Sodium Glutamate; D010404:Penicillin V