Construction of a quadroma to alpha-endorphin/horseradish peroxidase using an actinomycin D-resistant mouse myeloma cell line.
A hybrid hybridoma (quadroma), secreting antibodies with double specificity to alpha-endorphin (alpha-EP) and horseradish peroxidase (HRP), has been produced. The bispecific antibodies constituted about 28-29% of all immunologically active IgG, produced by quadroma. The quadroma was isolated by fusion of two mouse hybridomas (anti-HRP and anti-alpha-EP) with distinct phenotypes: double mutant AMDR/HAT(S), and wild type (AMDS/HATR). A novel strategy for the construction of a double-mutant was applied, based on the use of an actinomycin D-resistant (AMDR) mouse myeloma for initiation of one of the parental hybridomas.
Institute of Higher Nervous Activity and Neurophysiology, Russian Academy of Sciences, Moscow.
Massino YuS
E A
EA
Kizim
N N
NN
Dergunova
V M
VM
Vostrikov
A D
AD
Dmitriev
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D000918:Antibody Specificity; D002417:Cattle; D003609:Dactinomycin / Q000494:pharmacology; D004351:Drug Resistance; D004723:Endorphins / Q000276:immunology* / Q000378:metabolism; D006735:Horseradish Peroxidase / Q000276:immunology* / Q000378:metabolism; D006825:Hybridomas / Q000276:immunology*; D007124:Immunoenzyme Techniques; D051379:Mice; D009101:Multiple Myeloma / Q000276:immunology; D010902:Pituitary Gland / Q000378:metabolism; D051381:Rats; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000276:immunology; D018822:alpha-Endorphin
D000911:Antibodies, Monoclonal; D004723:Endorphins; D003609:Dactinomycin; D018822:alpha-Endorphin; D006735:Horseradish Peroxidase