pIF21, a versatile donor of transposons Tn1, Tn5 and Tn7 for tagging and mobilizing cryptic and non-selectable plasmids.
A plasmid, pIF21, has been constructed that is able to donate transposons Tn1, Tn5 and Tn7. The transposons are located on a temperature-sensitive derivative of the incP1 plasmid pRP1, which is transferable to a wide range of Gram-negative genera.
Department of Microbiology, Medical School, Newcastle upon Tyne, UK.
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Harwood
D003227:Conjugation, Genetic; D004251:DNA Transposable Elements; D006090:Gram-Negative Bacteria / Q000235:genetics*; D010957:Plasmids
D004251:DNA Transposable Elements