Resolution and productivity of conalbumin and lysozyme from fresh egg-white loaded at very high flow rate on a 250-mm length CM-HVFM column.
A 250 x 10 mm I.D. column of CM-HVFM, a novel carboxymethyl ion-exchange matrix, has been used as a preparative chromatographic column to separate fresh egg-white protein. When loading a diluted egg-white solution at pH 4.8 ovalbumin was not adsorbed and lysozyme was preferentially adsorbed compared to the conalbumin. As the column loading was increased from 24 to 450 kg m-3 column volume at the superficial velocity of 6.12 m h-1, the lysozyme continued to be absorbed eventually displacing conalbumin. A maximum lysozyme productivity at 16.7 kg m-3 h-1 was achieved at the highest loading. For conalbumin a maximum productivity of 8.8 kg m-3 h-1 occurred at the lower loading of 100 kg m-3. The purities of lysozyme and conalbumin were comparable at a column loading of 450 kg m-3 h-1. The performance of the column was not degraded, neither was the column blocked or channelled despite the high column loading at the high flow-rate.
School of Chemical Engineering, University of Bath, UK.
F
F
Ming
J A
JA
Howell
D000818:Animals; D001709:Biotechnology; D002645:Chickens; D002852:Chromatography, Ion Exchange / Q000379:methods*; D003207:Conalbumin / Q000302:isolation & purification*; D004527:Egg Proteins / Q000302:isolation & purification*; D009113:Muramidase / Q000302:isolation & purification*
D004527:Egg Proteins; D003207:Conalbumin; D009113:Muramidase