Experimental and theoretical evidence for convective nutrient transport in an immobilized cell support.
Even though immobilized-cell reactors possess several engineering advantages over free-cell reactors, their full potential has not been realized because mass transfer often limits the rate of nutrient supply and product removal from immobilized cell supports. We studied the interaction between mass transfer and reaction kinetics in the anaerobic conversion of glucose to CO2 and ethanol by yeast immobilized in a porous rotating disk on the agitator shaft of a conventional CSTR. A Sherwood number correlation was used to show that external mass-transfer resistances were negligible under typical operating conditions. The modulus of Weisz based on observable reaction parameters was used to gauge the importance of pore diffusion limitations. Under conditions for which significant pore diffusion effects and hence low effectiveness factors (eta = ca. 0.1) would be predicted, the observed reaction rates were much higher than expected (eta = ca. 1), suggesting that pore diffusion limitations were at least partially relieved by convective transport of glucose into the support. Two possible mechanisms of convective transport are discussed. We hypothesize that gas evolution was responsible for the convective enhancement of glucose supply.
Bringi
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V
Department of Agricultural and Chemical Engineering, Colorado State University, Fort Collins 80523.
Dale
B E
BE
D001709:Biotechnology / Q000295:instrumentation / Q000379:methods*; D002245:Carbon Dioxide / Q000378:metabolism; D002465:Cell Movement; D004058:Diffusion; D005947:Glucose / Q000378:metabolism; D007700:Kinetics; D008954:Models, Biological; D012440:Saccharomyces / Q000378:metabolism*
D002245:Carbon Dioxide; D005947:Glucose