Amplification of viral RNA for the detection of dengue types 1 and 2 virus.
In vitro DNA amplification by means of the polymerase chain reaction (PCR) was used to amplify dengue types 1 and 2 viral genomes in cultured cells and in the serum of persons infected with dengue virus. Results of the present investigation suggest that the PCR method is type-specific in detecting dengue virus and has a detection sensitivity of less than 100 plaque-forming units (pfu) for both serotypes of the virus. The PCR method may be useful for detecting and typing dengue virus in clinical and epidemiological specimens.
Pao
C C
CC
Department of Biochemistry, Chang Gung Medical College, Taipei, Taiwan, Republic of China.
Yao
D S
DS
Lin
C Y
CY
King
C C
CC
D001483:Base Sequence; D015139:Blotting, Southern; D003716:Dengue Virus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D016679:Genome, Viral; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000235:genetics*; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D014158:Transcription, Genetic / Q000235:genetics
D012367:RNA, Viral; D012194:RNA-Directed DNA Polymerase