Metal redistribution in largemouth bass (Micropterus salmoides) in response to restrainment stress and dietary cadmium: role of metallothionein and other metal-binding proteins.
1. Fish stressed by restrainment displayed elevated serum cortisol, copper and zinc levels; dietary cadmium had no effect. 2. Stress/dietary cadmium increased liver copper levels in a metal pool containing metallothionein and non-metallothionein proteins but decreased intestinal zinc bound as low molecular weight forms. 3. After restrainment, zinc losses occurred in dorsal skeletal muscle, ovary and spleen: copper decreased in intestine and pyloric caecum. 4. Dietary cadmium altered intestinal zinc distribution and raised hepatic Cu-binding protein levels but did not alter plasma zinc, copper or cortisol levels. 5. Alterations in zinc and copper concentrations during stress contrast with mammalian models.
Weber
D N
DN
Marine and Freshwater Biomedical Core Center, University of Wisconsin-Milwaukee 53201.
Eisch
S
S
Spieler
R E
RE
Petering
D H
DH
D000818:Animals; D001492:Bass / Q000378:metabolism*; D002104:Cadmium / Q000008:administration & dosage / Q000494:pharmacology*; D002352:Carrier Proteins / Q000378:metabolism; D003300:Copper / Q000097:blood / Q000378:metabolism*; D004032:Diet; D005260:Female; D006854:Hydrocortisone / Q000097:blood; D007413:Intestinal Mucosa / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008668:Metallothionein / Q000378:metabolism*; D009132:Muscles / Q000378:metabolism; D010053:Ovary / Q000378:metabolism; D012149:Restraint, Physical; D013154:Spleen / Q000378:metabolism; D013312:Stress, Physiological / Q000378:metabolism*; D014018:Tissue Distribution; D015032:Zinc / Q000097:blood / Q000378:metabolism*
D002352:Carrier Proteins; C023548:copper-binding protein; D002104:Cadmium; D003300:Copper; D008668:Metallothionein; D015032:Zinc; D006854:Hydrocortisone