Stereospecific effects of ascorbic acid and analogues on D1 and D2 agonist binding.
Ascorbic acid inhibited the specific binding of both the D1 agonist, [3H] SKF 38393, and the D2 agonist, [3H] N-0437 at physiologically relevant concentrations. This inhibition was both stereospecific and receptor selective. Using ligand concentrations approximating their KD's, the IC50's for ascorbate and two structural analogues, isoascorbate and D-glucoascorbate, were determined. The rank order of IC50's at both D1 and D2 were D-glucoascorbate greater than isoascorbate greater than ascorbate. However, the IC50 for each compound was greater at D1 than D2. Evaluation of the relationship between the IC50 for ascorbate and the ligand concentration using both the D1 and the D2 ligand yielded data inconsistent with competitive inhibition models. Preliminary experiments were conducted to evaluate the site and type of inhibition with results consistent with an allostearic effect at the level of the receptor.
Department of Psychiatry and Behavioral Neurobiology, University of Alabama, Birmingham 35294-0017.
L C
LC
Tolbert
P E
PE
Morris
J J
JJ
Spollen
S C
SC
Ashe
D015647:2,3,4,5-Tetrahydro-7,8-dihydroxy-1-phenyl-1H-3-benzazepine / Q000037:antagonists & inhibitors* / Q000378:metabolism; D000818:Animals; D001205:Ascorbic Acid / Q000031:analogs & derivatives / Q000494:pharmacology*; D003342:Corpus Striatum / Q000187:drug effects / Q000378:metabolism; D015259:Dopamine Agents / Q000037:antagonists & inhibitors* / Q000378:metabolism; D006539:Heptoses / Q000494:pharmacology; D007700:Kinetics; D008024:Ligands; D008297:Male; D051381:Rats; D011954:Receptors, Dopamine / Q000378:metabolism*; D013237:Stereoisomerism; D013764:Tetrahydronaphthalenes / Q000037:antagonists & inhibitors* / Q000378:metabolism; D013876:Thiophenes / Q000037:antagonists & inhibitors* / Q000378:metabolism
D015259:Dopamine Agents; D006539:Heptoses; D008024:Ligands; D011954:Receptors, Dopamine; D013764:Tetrahydronaphthalenes; D013876:Thiophenes; C005056:isoascorbic acid; C007850:glucoascorbic acid; D015647:2,3,4,5-Tetrahydro-7,8-dihydroxy-1-phenyl-1H-3-benzazepine; C047508:rotigotine; D001205:Ascorbic Acid