Intergeneric protoplast fusion between Gluconobacter oxydans and Corynebacterium species.
Intergeneric protoplast fusion between 2,5-diketo-gluconic acid producing Gluconobacter oxydans (ATCC 9937) and a mutant strain of Corynebacterium species (ATCC 31090), capable of reducing 2,5-diketo-gluconic acid to 2-keto-L-gulonic acid, a penultimate step in vitamin C production) resulted in viable recombinants. Some of the fusion products exhibited the capacity to convert D-glucose to 2-keto-L-gulonic acid, but the conversion rate is low.
Genetic Engineering Unit, Regional Research Laboratory, Jammu Tawi, India.
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D016947:Acetobacteraceae / Q000235:genetics* / Q000378:metabolism; D001431:Bacteriological Techniques; D003352:Corynebacterium / Q000235:genetics* / Q000378:metabolism; D005818:Genetic Engineering / Q000379:methods; D011523:Protoplasts / Q000382:microbiology*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis*; D013045:Species Specificity
D011993:Recombinant Fusion Proteins