Selective adherence of neurons and glial cells from dissociated cerebral and spinal cord microcarrier cultures.
In stationary cultures of dissociated brain and spinal cord grown on microcarriers (MCs), the neuronal and ependymal cells attached to the MCs forming floating aggregates in which they grow in a three-dimensional pattern. The glial and meningeal elements on the contrary, tend to dissociate from the aggregates and adhere to the plastic dish where they divide to form a monolayer. This different behavior of CNS components is not observed in rotating cultures in which all CNS cells remain attached to the MCs and develop into mature floating structures. This cell separation in stationary MC-cultures which is documented here by SEM and immunocytochemistry, may be useful for analysis and evaluation of the metabolic biochemical events of each of the cellular components derived from the same culture.
Shahar
A
A
Department of Virology, Israel Institute for Biological Research, Ness-Ziona.
Reuveny
S
S
David
Y
Y
Hamdorf
G
G
Terborg
M
M
Cervos-Navarro
J
J
D000818:Animals; D001921:Brain / Q000166:cytology* / Q000648:ultrastructure; D002448:Cell Adhesion / Q000502:physiology; D002455:Cell Division; D002469:Cell Separation / Q000379:methods; D002478:Cells, Cultured; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D007124:Immunoenzyme Techniques; D008863:Microspheres; D009457:Neuroglia / Q000166:cytology* / Q000648:ultrastructure; D009474:Neurons / Q000166:cytology* / Q000648:ultrastructure; D010751:Phosphopyruvate Hydratase / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013116:Spinal Cord / Q000166:cytology* / Q000648:ultrastructure
D005904:Glial Fibrillary Acidic Protein; D010751:Phosphopyruvate Hydratase