Effects of glycerol on the growth, adhesion, and cellulolytic activity of rumen cellulolytic bacteria and anaerobic fungi.
The effect of glycerol on the growth, adhesion, and cellulolytic activity of two rumen cellulolytic bacterial species, Ruminococcus flavefaciens and Fibrobacter succinogenes subsp. succinogenes, and of an anaerobic fungal species, Neocallimastix frontalis, was studied. At low concentrations (0.1-1%), glycerol had no effect on the growth, adhesion, and cellulolytic activity of the two bacterial species. However, at a concentration of 5%, it greatly inhibited their growth and cellulolytic activity. Glycerol did not affect the adhesion of bacteria to cellulose. The growth and cellulolytic activity of N. frontalis were inhibited by glycerol, increasingly so at higher concentrations. At a concentration of 5%, glycerol totally inhibited the cellulolytic activity of the fungus. Thus, glycerol can be added to animal feed at low concentrations.
Laboratory of Microbiology, INRA, C.R. Saint-Genes-Champanelle, France.
V
V
Roger
G
G
Fonty
C
C
Andre
P
P
Gouet
D000818:Animals; D001421:Bacteria, Anaerobic / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D001422:Bacterial Adhesion / Q000187:drug effects; D001439:Bacteroides / Q000187:drug effects; D002448:Cell Adhesion / Q000187:drug effects; D002482:Cellulose / Q000378:metabolism*; D005658:Fungi / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D005990:Glycerol / Q000494:pharmacology*; D007700:Kinetics; D012417:Rumen / Q000382:microbiology
D002482:Cellulose; D005990:Glycerol