Identification of human blood in mosquitoes (Diptera: Culicidae) using nonradioactive DNA dot blot hybridization.
A dot blot hybridization procedure was developed to detect human blood meals in engorged mosquitoes. A biotinylated DNA probe allowed the detection of 10-100 ng of human DNA, discriminated well between human and nonhuman sources of blood, and cross-reacted only with monkey DNA. Results showed that this method was a specific and sensitive technique for the identification of blood meals up to 100 h after ingestion. The nonisotopic label offers easy handling without the problems inherent in the use of radioisotopes, and it can be adapted for use in routine field tests.
Sato
C
C
Department of Biology, Kagawa Medical School, Japan.
Furuya
Y
Y
Harada
M
M
Suguri
S
S
D000818:Animals; D009033:Culicidae; D004247:DNA / Q000097:blood* / Q000235:genetics; D015342:DNA Probes; D005914:Globins / Q000235:genetics; D006801:Humans; D007303:Insect Vectors; D009693:Nucleic Acid Hybridization; D013045:Species Specificity
D015342:DNA Probes; D005914:Globins; D004247:DNA