Isolation of mutants with improved production of cAMP from Microbacterium sp. no. 205 (ATCC 21376).
Mutants were isolated from Microbacterium sp. no. 205 (ATCC 21376) producing 13-30 mM cyclic adenosine 3',5'-monophosphate (cAMP) by salvage biosynthesis, through sequential improvements of the bacterium for the purpose of improving cAMP production. The mutants produced 50-75 mM cAMP on 100 mM inosine 5'-monophosphate as a precursor. Mutants resistant to the inhibition of growth by cAMP at high concentrations were isolated; the resistance was one of four characteristics effective for improved production of cAMP.
Ishiyama
J
J
Research and Development Division, Kikkoman Corporation, Chiba-ken, Japan.
D003352:Corynebacterium / Q000187:drug effects / Q000235:genetics / Q000254:growth & development; D000242:Cyclic AMP / Q000096:biosynthesis* / Q000235:genetics; D005947:Glucose / Q000494:pharmacology; D016296:Mutagenesis; D011687:Purines / Q000494:pharmacology; D011743:Pyrimidines / Q000494:pharmacology; D012680:Sensitivity and Specificity
D011687:Purines; D011743:Pyrimidines; D000242:Cyclic AMP; D005947:Glucose