S-Zephyr, a new high performance ion exchange chromatography column matrix.
S-Zephyr, a new column material for high performance cation exchange chromatography of proteins, is compared with Mono-S. The comparison is based on a retentivity study, a model separation of an artificial protein mixture, a sample load capacity experiment and the development of the separation performance at a column overload situation. S-Zephyr is found to be a good matrix for cation exchange chromatography, for analytical separations as well as for small and large scale protein purification applications.
Rongen
R M
RM
Department of Chemistry, Baylor University, Waco, TX 767698-7348.
Adler
C M
CM
Scouten
W H
WH
D000837:Anion Exchange Resins; D001709:Biotechnology; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002919:Chymotrypsinogen / Q000302:isolation & purification; D003574:Cytochrome c Group / Q000302:isolation & purification; D005069:Evaluation Studies as Topic; D006454:Hemoglobins / Q000302:isolation & purification; D006801:Humans; D009113:Muramidase / Q000302:isolation & purification; D011506:Proteins / Q000302:isolation & purification*
D000837:Anion Exchange Resins; D003574:Cytochrome c Group; D006454:Hemoglobins; D011506:Proteins; D002919:Chymotrypsinogen; D009113:Muramidase