Localization of methanol dehydrogenase in two strains of methylotrophic bacteria detected by immunogold labeling.
Antibodies to methanol dehydrogenase purified from Methylobacterium sp. strain AM1 and Methylomonas sp. strain A4 were raised. The antibody preparations were used in indirect immunogold labeling studies. With this approach, methanol dehydrogenase was found to be preferentially localized to the periplasmic region of the methylotroph Methylobacterium sp. strain AM1 and to the intracytoplasmic membrane of the methanotroph Methylomonas sp. strain A4. Antibody cross-reactivity to other methylotrophic bacteria was detected.
Fassel
T A
TA
Department of Biological Sciences, University of Wisconsin-Milwaukee 53201.
Buchholz
L A
LA
Collins
M L
ML
Remsen
C C
CC
D000429:Alcohol Oxidoreductases / Q000378:metabolism*; D002462:Cell Membrane / Q000201:enzymology; D006088:Gram-Negative Aerobic Bacteria / Q000201:enzymology* / Q000648:ultrastructure; D007150:Immunohistochemistry; D008772:Methylococcaceae / Q000201:enzymology* / Q000648:ultrastructure; D016253:Microscopy, Immunoelectron; D013045:Species Specificity
D000429:Alcohol Oxidoreductases; C019641:alcohol dehydrogenase (acceptor)