Characterization and molecular cloning of Bifidobacterium longum cryptic plasmid pMB1.
The small cryptic plasmid pMB1 (1.9 kb), previously isolated from Bifidobacterium longum, has been characterized by physical mapping. Two cloning vectors, pMR3 and pDG7, carrying chloramphenicol and ampicillin resistances derived from pJH101, have been electroporated in Escherichia coli.
Department of Pharmaceutical Sciences, University of Bologna, Italy.
D
D
Matteuzzi
P
P
Brigidi
M
M
Rossi
D
D
Di
D001644:Bifidobacterium / Q000235:genetics*; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000032:analysis*; D004587:Electrophoresis, Agar Gel; D005822:Genetic Vectors; D010957:Plasmids; D015183:Restriction Mapping; D014169:Transformation, Bacterial
D004269:DNA, Bacterial