Mapping of functional regions of Clostridium perfringens type A enterotoxin.
Studies were conducted to allow construction of an initial map of the structure-versus-function relationship of the Clostridium perfringens type A enterotoxin (CPE). Removal of the N-terminal 25 amino acids of CPE increased the primary cytotoxic effect of CPE but did not affect binding. CPE sequences required for at least four epitopes were also identified.
Department of Molecular Genetics and Biochemistry, University of Pittsburgh School of Medicine, Pennsylvania 15261-2072.
P C
PC
Hanna
E U
EU
Wieckowski
T A
TA
Mietzner
B A
BA
McClane
D000911:Antibodies, Monoclonal / Q000276:immunology; D003016:Clostridium perfringens / Q000378:metabolism*; D004768:Enterotoxins / Q000737:chemistry / Q000276:immunology / Q000633:toxicity*; D000939:Epitopes / Q000032:analysis; D010446:Peptide Fragments / Q000276:immunology / Q000633:toxicity; D013329:Structure-Activity Relationship
D000911:Antibodies, Monoclonal; D004768:Enterotoxins; D000939:Epitopes; D010446:Peptide Fragments; C035598:enterotoxin, Clostridium