Erythropoietin gene expression in haemopoietic cell lines.
Erythropoietin (Epo) gene expression was studied in a number of different haemopoietic cell lines by in situ hybridization and Northern Blot analysis using a radioisotope-labelled monkey Epo DNA probe. A positive message was expressed by a human cell line, CM-S, derived from a patient with congenital hypoplastic anemia, and by a murine erythro-leukaemic cell line, clone 707, derived from the spleen of Friend virus-infected mice. No message was detected in two megakaryoblastic cell lines, and in a monocytic cell line, derived from a patient with acute monocytic leukaemia. These data may fit with the hypothesis of expression of Epo and other growth factors by haemopoietic cells through a mechanism of so-called autocrine secretion.
Division of Haematology, UMDS-St. Thomas' Campus, London, U.K.
E
E
Abood
S
S
Eridani
B
B
Sawyer
N
N
Westwood
T C
TC
Pearson
D000818:Animals; D015152:Blotting, Northern; D002460:Cell Line; D004921:Erythropoietin / Q000235:genetics*; D015870:Gene Expression; D006412:Hematopoietic Stem Cells / Q000378:metabolism*; D006801:Humans; D016130:Immunophenotyping; D051379:Mice; D009693:Nucleic Acid Hybridization; D014407:Tumor Cells, Cultured
D004921:Erythropoietin