Transitory expression of Thy-1 antigen in immature B cell lines.
In cmu- B220+ Thy-1- murine immature B cells transformed with a temperature-sensitive mutant of Abelson murine leukemia virus, a low level of Thy-1 antigen was transitorily expressed after the shift of the culture temperature from the permissive (35 degrees C) to the non-permissive (39 degrees C) temperature. On the other hand, B220 antigen was persistently expressed regardless of whether the cells were cultured at the permissive or non-permissive temperature. No other T-lineage-specific antigens, CD3, CD4, and CD8 were induced during the culture at the non-permissive temperature. Expression of Thy-1 antigen was confirmed by the detection of a low level of Thy-1-specific mRNA. These results clearly showed that immature B cells could express Thy-1 antigen during proliferation and/or differentiation. The results imply a role for Thy-1 antigen in B cell proliferation and/or differentiation.
Oka
Y
Y
Department of Medicine III, Osaka University Medical School, Japan.
Shimizu
Y
Y
Tsukada
S
S
Sugiyama
H
H
D000818:Animals; D000954:Antigens, Surface / Q000378:metabolism*; D001402:B-Lymphocytes / Q000276:immunology*; D002454:Cell Differentiation; D002455:Cell Division; D002460:Cell Line; D002461:Cell Line, Transformed / Q000276:immunology; D005434:Flow Cytometry; D008562:Membrane Glycoproteins / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D012333:RNA, Messenger / Q000378:metabolism; D018800:Thy-1 Antigens
D000954:Antigens, Surface; D008562:Membrane Glycoproteins; D012333:RNA, Messenger; D018800:Thy-1 Antigens