Potential- and acetylcholine-activated ionic currents of pheochromocytoma PC12 cells during incubation with nerve growth factor.
Pheochromocytoma PC12 cells incubated with and without nerve growth factor were investigated using the patch-clamp technique in the whole-cell recording mode, and the concentration clamp method in the rat. On the fourth day of incubation with nerve growth factor, sodium potential-activated ionic currents appeared in the membranes of the most morphologically differentiated cells. At the same period a three-fold increase of acetylcholine-activated current density, compared with the cells incubated without nerve growth factor, was observed. Thus, the qualitative and quantitative changes in membrane properties can be a result of metabolic reorganization in PC12 cells induced by nerve growth factor and accompanied by morphological differentiation according to neuronal phenotype.
Department of Physiology of the Spinal Cord, A. A. Bogomoletz Institute of Physiology, Academy of Sciences, Ukrainian SSR, Kiev.
K V
KV
Baev
V K
VK
Beresovskii
V N
VN
Kalunov
N I
NI
Luschitskaya
K I
KI
Rusin
Vilner BYa
T V
TV
Zavadskaya
D000109:Acetylcholine / Q000494:pharmacology*; D000310:Adrenal Gland Neoplasms; D000818:Animals; D004305:Dose-Response Relationship, Drug; D005071:Evoked Potentials / Q000187:drug effects; D007473:Ion Channels / Q000187:drug effects / Q000502:physiology*; D007700:Kinetics; D009414:Nerve Growth Factors / Q000494:pharmacology*; D016716:PC12 Cells; D010673:Pheochromocytoma; D015221:Potassium Channels / Q000187:drug effects / Q000502:physiology; D015222:Sodium Channels / Q000187:drug effects / Q000502:physiology; D013779:Tetrodotoxin / Q000494:pharmacology; D013997:Time Factors
D007473:Ion Channels; D009414:Nerve Growth Factors; D015221:Potassium Channels; D015222:Sodium Channels; D013779:Tetrodotoxin; D000109:Acetylcholine