Cultivation of recombinant E. coli and production of fusion protein in 60-1 bubble column and airlift tower loop reactors.
E. coli K12 with multicopy plasmid (lambda PR-promoter and temperature-sensitive lambda cI 857 repressor) was cultivated in 60-l bubble column and airlift tower loop reactors. The medium composition, cell concentration, and intracellulary enzyme activity were monitored on-line during batch, fed-batch, and continuous cultivations. The specific growth rates, cell mass yield coefficients, plasmid stabilities, productivities of the amount of active fusion protein (beta-galactosidase activity), concentrations and yields of acetic acid, and volumetric oxygen transfer coefficient were evaluated for different medium compositions and cultivation conditions. The enzyme activity was also monitored during the temperature induction. The results evaluated in the 60-l bubble column and airlift tower loop reactors are compared with those evaluated in a 1-1 stirred-tank reactor.
Kracke-Helm
H A
HA
Institute für Technische Chemie, Universität Hannover, Germany.
Rinas
U
U
Hitzmann
B
B
Schügerl
K
K
D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification; D001431:Bacteriological Techniques / Q000295:instrumentation*; D003001:Cloning, Molecular / Q000379:methods*; D003470:Culture Media / Q000494:pharmacology; D004790:Enzyme Induction; D004867:Equipment Design; D004926:Escherichia coli / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D005818:Genetic Engineering / Q000295:instrumentation*; D005822:Genetic Vectors; D005990:Glycerol / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism; D011489:Protein Denaturation; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis* / Q000302:isolation & purification; D013696:Temperature; D001616:beta-Galactosidase / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification
D001426:Bacterial Proteins; D003470:Culture Media; D011993:Recombinant Fusion Proteins; D001616:beta-Galactosidase; D005990:Glycerol; D010100:Oxygen