Development of a piezoelectric immunosensor for the detection of enterobacteria.
A piezoelectric crystal immunosensor has been developed for the detection of enterobacteria in drinking water using antibodies against the enterobacterial common antigen. Applying an anti-enterobacterial antibody layer via protein A immobilization onto a 10-MHz crystal, a response is observed for 10(6) to 10(9) cells ml-1 of Escherichia coli K12, and for various other antigens of the Enterobacteriaceae family.
Department of Botany, Technische Universität München at Weihenstephan, Freising, Germany.
M
M
Plomer
G G
GG
Guilbault
B
B
Hock
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial / Q000032:analysis*; D015374:Biosensing Techniques; D004755:Enterobacteriaceae / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans; D007118:Immunoassay / Q000379:methods; D014871:Water Microbiology; D014881:Water Supply / Q000592:standards*
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; C017286:enterobacterial common antigen