Production of a foreign protein product with genetically modified plant cells.
Plant cells (Nicotiana tabacum) were genetically engineered to produce a foreign protein, chloramphenicol acetyltransferase (CAT), and the CAT production from suspension cultures was investigated. Suspension cultures were grown in a shake flask, a stirred fermenter, and a bubble-column fermenter. The CAT production was growth related and the maximum activity was reached during the early stationary phase. A 41-day, semicontinuous stirred fermenter run, consisting of five sequential batch runs, demonstrated long-term CAT production. Continuous CAT production was also accomplished in a bubble-column fermenter at a medium flow rate of 3.1 ml h-1, which was equivalent to a dilution rate of 0.25 day-1.
Hogue
R S
RS
Department of Chemical Engineering, Washington State University, Pullman 99164.
Lee
J M
JM
An
G
G
D002478:Cells, Cultured; D015500:Chloramphenicol O-Acetyltransferase / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D005285:Fermentation; D005818:Genetic Engineering / Q000379:methods*; D007700:Kinetics; D010947:Plants, Toxic; D012231:Rhizobium / Q000201:enzymology / Q000235:genetics; D014026:Nicotiana / Q000235:genetics* / Q000254:growth & development
D015500:Chloramphenicol O-Acetyltransferase