Heterologous gene expression in Hansenula polymorpha: efficient secretion of glucoamylase.
We have introduced the glucoamylase gene (GAM1) from Schwanniomyces occidentalis into the genome of the methylotrophic yeast Hansenula polymorpha to study the potential of this organism as a host for high-level expression of a heterologous gene encoding a secretory protein. Transformants of H. polymorpha containing GAM1 under control of the formate dehydrogenase (FMD) promoter are stable and efficiently secrete an active glucoamylase that is faithfully processed and modified. Yields of up to 1.4g/l of active enzyme were obtained at cell densities of 100-130 grams dry weight per liter.
Gellissen
G
G
Rhein Biotech GmbH, Düsseldorf, FRG.
Janowicz
Z A
ZA
Merckelbach
A
A
Piontek
M
M
Keup
P
P
Weydemann
U
U
Hollenberg
C P
CP
Strasser
A W
AW
D000595:Amino Acid Sequence; D003001:Cloning, Molecular / Q000379:methods*; D005285:Fermentation; D005800:Genes, Fungal; D005087:Glucan 1,4-alpha-Glucosidase / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D010843:Pichia / Q000201:enzymology / Q000235:genetics*; D010957:Plasmids; D004718:Saccharomycetales / Q000201:enzymology*
D005087:Glucan 1,4-alpha-Glucosidase