The micronucleus assay with mouse peripheral blood reticulocytes using acridine orange-coated slides with triethylenemelamine.
A micronucleus assay using mouse peripheral blood and supravital staining with acridine orange (AO) was validated by two laboratories on triethylenemelamine-treated mice. Dose- and time-dependent increases in micronucleated peripheral reticulocytes were observed. This new method can be used as an alternative to the conventional bone marrow micronucleus assay.
Toxicology Laboratories, Yoshitomi Pharmaceutical Industries, Ltd., Fukuoka-ken, Japan.
E
E
Yamamura
H
H
Hirono
M
M
Takeuchi
M
M
Kojima
S
S
Aoki
D000165:Acridine Orange; D000818:Animals; D004305:Dose-Response Relationship, Drug; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D015162:Micronucleus Tests / Q000379:methods; D016685:Mitomycin / Q000633:toxicity; D009153:Mutagens / Q000633:toxicity*; D012156:Reticulocytes / Q000187:drug effects*; D014265:Triethylenemelamine / Q000633:toxicity*
D009153:Mutagens; D016685:Mitomycin; D000165:Acridine Orange; D014265:Triethylenemelamine