Isolation and characterization of a cryptic plasmid from mesophilic aeromonads: potential as a cloning vector.
A 2.6 kb covalently closed circular plasmid has been isolated from clinical and environmental isolates of Aeromonas sobria and A. hydrophila. The possibility that the plasmid carries genetic determinants that mediate resistance to a variety of anti-microbial agents has been eliminated. The plasmid is stable at approximately 20-25 copies per chromosome equivalent which, together with its relatively small size and the presence of unique restriction sites, makes it a good candidate for development as a cloning vector.
Department of Microbiology, University of Reading, UK.
I F
IF
Connerton
J S
JS
Kaur
S
S
Rogers
R W
RW
Park
D000333:Aeromonas / Q000235:genetics*; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000032:analysis*; D004587:Electrophoresis, Agar Gel; D005822:Genetic Vectors; D010957:Plasmids; D015183:Restriction Mapping
D004269:DNA, Bacterial