The complete nucleotide sequence of region 1 of the CFA/I fimbrial operon of human enterotoxigenic Escherichia coli.
The production of the plasmid-encoded fimbrial antigen CFA/I of enterotoxigenic Escherichia coli requires two DNA regions: CFA/I region 1 and CFA/I region 2. These two regions are separated by about 40 kb on the wildtype plasmid. CFA/I region 1 contains the structural genes, whereas CFA/I region 2 contains a positive regulator. The first two genes (cfaA and cfaB) and the cfaD' sequence of region 1 have already been described. Here the total nucleotide sequence of region 1 is presented. Two new genes in region 1 are described, named cfaC and cfaE. The GC content of the genes in region 1 is 33.6% which is substantially lower than normally found in E. coli genes (50%). The codon usage also differs from the standard codons used in E. coli.
Jordi
B J
BJ
Department of Bacteriology, Faculty of Veterinary Medicine, University of Utrecht, The Netherlands.
Willshaw
G A
GA
van der Zeijst
B A
BA
Gaastra
W
W
D000595:Amino Acid Sequence; D000942:Antigens, Bacterial / Q000235:genetics*; D001482:Base Composition; D001483:Base Sequence; D003062:Codon / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D004768:Enterotoxins / Q000096:biosynthesis; D004926:Escherichia coli / Q000235:genetics* / Q000276:immunology / Q000472:pathogenicity; D036781:Fimbriae Proteins; D010861:Fimbriae, Bacterial / Q000276:immunology; D006801:Humans; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D009876:Operon
D000942:Antigens, Bacterial; D003062:Codon; D004269:DNA, Bacterial; D004768:Enterotoxins; C022924:colonization factor antigens; D036781:Fimbriae Proteins