Improved processes for the production and isolation of dynemicin A and large-scale fermentation in a 10,000-liter fermentor.
Supplementing the culture of Micromonospora chersina sp. nov. No. M956-1 with NaI (0.5 mg/l) enhanced the production of dynemicin A by 35-fold in shake flask culture. Homogeneous dynemicin A was obtained from the whole broth extract by Dicalite chromatography, Sephadex LH-20 chromatography and vacuum liquid chromatography. Gram quantities of dynemicin A were obtained from the fermentation of M. chersina sp. nov. No. M956-1 in a 10,000-liter fermentor.
Lam
K S
KS
Bristol-Myers Squibb Company, Pharmaceutical Research Institute, Wallingford, CT 06492.
Titus
J A
JA
Dabrah
T T
TT
Kimball
D L
DL
Veitch
J M
JM
Gustavson
D R
DR
Compton
B J
BJ
Matson
J A
JA
Forenza
S
S
Ross
J
J
D000880:Anthraquinones / Q000302:isolation & purification / Q000378:metabolism; D000903:Antibiotics, Antineoplastic / Q000096:biosynthesis* / Q000302:isolation & purification; D003470:Culture Media; D053281:Enediynes; D005285:Fermentation; D008847:Micromonospora / Q000378:metabolism
D000880:Anthraquinones; D000903:Antibiotics, Antineoplastic; D003470:Culture Media; D053281:Enediynes; C061229:dynemicin A