HPLC-monitoring of AZT in HIV-infected patient's plasma: a critical study.
3'-azido-2', 3'-deoxythymidine (AZT) concentrations in spiked human plasma were determined by means of reversed-phase high performance liquid chromatography (RP-HPLC). Samples were first cleaned-up for analysis using solid-phase extraction (SPE) columns filled with Silipore C18. In the concentration range comprising usual peak plasma concentrations during AZT therapy (0.1-20 mumol/l, i.e. 0.026-5.34 micrograms/ml) mean efficiency of the extraction procedure reached as high as 75.3% of original AZT concentrations in standard unextracted aqueous solutions. Replicate analyses in this range gave satisfactory intra-assay precision and reproducibility with coefficient of variation less than 11.3%. Calibration curves both in water and plasma showed good linearity (r > 0.999). The detection limit in plasma was 2 mumol/l, i.e. 5.3 ng per a 20 microliter of sample injected to the HPLC column. Plasma levels of AZT after a single dose administration, determined by HPLC and RIA showed rather poor correlation (r = 0.8900). In RIA about 1.7-4.5 times higher concentration values were obtained in a relatively short time, and, consequently, this method may better fulfil the needs of routine drug monitoring.
Borvák
J
J
Institute of Virology, Slovak Academy of Sciences, Bratislava.
Kasanická
J
J
Mayer
V
V
D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D015658:HIV Infections / Q000097:blood* / Q000188:drug therapy; D006801:Humans; D012015:Reference Standards; D015203:Reproducibility of Results; D015215:Zidovudine / Q000031:analogs & derivatives / Q000097:blood* / Q000493:pharmacokinetics
D015215:Zidovudine; C055886:3'-azido-3'-deoxy-5'-O-beta-glucopyranuronosylthymidine