Conjugative gene transfer in marine cyanobacteria: Synechococcus sp., Synechocystis sp. and Pseudanabaena sp.
Versatility of gene transfer by transconjugation in marine cyanobacteria was demonstrated. In this study, seven different marine cyanobacteria were used as recipient cells. First, transconjugation was carried out using the mobilizable transposon (Tn5) carrying plasmid pSUP1021. Transconjugates were observed in all marine cyanobacteria tested. Second, the broad-host-range vector pKT0230 (IncQ) was tested for transconjugation. pKT230 has been successfully transferred in a marine cyanobacterium Synechococcus sp. NKBG15041C, and replicated as an autonomous replicon without alteration in the restriction enzyme pattern. A maximum transfer efficiency of 5.2 x 10(-4) transconjugants/recipient cell was observed, when mating was performed on agar plates containing low salinity (0.015 M NaCl) medium. This is the first study to demonstrate gene transfer in marine cyanobacteria via transconjugation.
Department of Biotechnology, Faculty of Technology, Tokyo University of Agriculture and Technology, Japan.
K
K
Sode
M
M
Tatara
H
H
Takeyama
J G
JG
Burgess
T
T
Matsunaga
D001709:Biotechnology; D003227:Conjugation, Genetic; D000458:Cyanobacteria / Q000235:genetics*; D005818:Genetic Engineering; D005822:Genetic Vectors; D010957:Plasmids; D013045:Species Specificity