Dye-ligand centrifugal affinity chromatography.
A fast method for the screening of a large number of immobilized dyes for the purification or binding of proteins called dye-ligand centrifugal affinity chromatography, is described. The ease and speed of this method is demonstrated by screening 65 immobilized dyes for the binding of purified goat IgG. Two immobilized dyes (Drimarene Blue K-R and Drimarene Rubine R/K-5BL) with a high affinity for goat IgG were found to bind specifically the Fc-fragment of the IgG.
Department of Chemistry, Baylor University, Waco, TX 76798-7348.
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D000818:Animals; D002498:Centrifugation / Q000379:methods; D002846:Chromatography, Affinity / Q000379:methods*; D004396:Coloring Agents; D004591:Electrophoresis, Polyacrylamide Gel; D006041:Goats; D007128:Immunoglobulin Fragments / Q000302:isolation & purification; D007074:Immunoglobulin G / Q000302:isolation & purification; D008024:Ligands; D010206:Papain; D011506:Proteins / Q000302:isolation & purification*; D012685:Sepharose
D004396:Coloring Agents; D007128:Immunoglobulin Fragments; D007074:Immunoglobulin G; D008024:Ligands; D011506:Proteins; D012685:Sepharose; D010206:Papain