Immunoelectron microscopic localization of thiolases, beta-oxidation enzymes of an n-alkane-utilizable yeast, Candida tropicalis.
The location of acetoacetyl-CoA thiolase (T-I) and 3-ketoacyl-CoA thiolase (T-III), enzymes of the fatty acid beta-oxidation system, was studied in n-alkane-grown Candida tropicalis cells by immunoelectron microscopy using a post-embedding method with colloidal gold conjugated IgG. The deposition of gold particles for T-I was detected in the microbodies and cytoplasm and that of gold particles for T-III specifically in the microbodies. The double labeling technique confirmed that T-I and T-III occurred concurrently in a microbody and T-I also in cytoplasm. These results were consistent with the biochemical data based on subcellular fractionation and indicated that the yeast beta-oxidation system operates efficiently only in the microbodies.
Department of Chemical and Biological Sciences, Faculty of Science, Japan Women's University, Tokyo.
N
N
Kamasawa
N
N
Naito
T
T
Kurihara
Y
Y
Kamada
M
M
Ueda
A
A
Tanaka
M
M
Osumi
D000101:Acetyl-CoA C-Acetyltransferase / Q000032:analysis*; D000102:Acetyl-CoA C-Acyltransferase / Q000032:analysis*; D000473:Alkanes; D002175:Candida / Q000201:enzymology*; D003593:Cytoplasm / Q000201:enzymology*; D008830:Microbodies / Q000201:enzymology*; D016253:Microscopy, Immunoelectron; D010084:Oxidation-Reduction
D000473:Alkanes; D000102:Acetyl-CoA C-Acyltransferase; D000101:Acetyl-CoA C-Acetyltransferase