Cross-reactivity of bacteriocins from lactic acid bacteria and lantibiotics in a nisin bioassay and ELISA.
A number of bacteriocins from lactic acid bacteria and lantibiotics were tested for cross-reactivity in a nisin ELISA and bioassay. The bacteriocins showed no cross-reactivity, reflecting their structural dissimilarity from nisin. The lantibiotic subtilin which shares many common structural features with nisin, showed a high cross-reactivity in both the ELISA and the bioassay suggesting possible modifications to nisin to enhance its activity. Gallidermin did not cross react in the ELISA but did produce a zone of inhibition in the less specific bioassay. Other lantibiotics tested did not react in either assay.
School of Biological Sciences, University of Surrey, Guildford, UK.
M B
MB
Falahee
M R
MR
Adams
D000900:Anti-Bacterial Agents / Q000032:analysis*; D001419:Bacteria / Q000378:metabolism; D001430:Bacteriocins / Q000032:analysis*; D001681:Biological Assay; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D007773:Lactates / Q000096:biosynthesis; D019344:Lactic Acid; D009561:Nisin / Q000032:analysis*
D000900:Anti-Bacterial Agents; D001430:Bacteriocins; D007773:Lactates; D009561:Nisin; D019344:Lactic Acid