Somatotopic organization of tyrosine hydroxylase expression in the rat locus coeruleus: long term effect of RU24722.
Tyrosine hydroxylase (TH) tissue concentration was determined by immunostaining of tissue sections directly transferred onto nitrocellulose membranes in the restricted region of the noradrenergic perikarya of the locus coeruleus (LC) along its postero-anterior axis. TH containing cells were systematically counted on adjacent post fixed sections stained by immunohistochemistry. The absolute quantity of TH was estimated in each section and was found to be linearly related to the number of TH immuno-positive cells found in the adjacent section. The ratio between these two parameters was thus used as an index of the cellular concentration of TH in noradrenergic cells. In the LC of control rats, the TH cellular concentration was lower (-39%) in the anterior than in the posterior half of the structure. Three days after an injection of 20 mg/kg of RU24722, an eburnamine derivative known to increase the quantity of TH in the LC, increases in quantities of TH were found in both portions of the LC. Moreover in the posterior LC the increase in the amount of TH resulted from a significant increase in the number of TH-immunopositive cells. In the anterior part, however, it was primarily the result of a significant increase in TH cellular concentration. Throughout the LC there was an increase in the cellular concentration of TH which was inversely proportional to the concentrations found in control animals. TH mRNA content was measured by a quantitative in situ hybridization in sections of both the posterior and anterior LC one day after a single injection of RU24722 at the same dose. The quantity of TH mRNA was significantly increased in both parts. The number of TH mRNA-expressing neurons also increased, especially in the anterior LC. Thus the effects at the level of TH protein and TH mRNA were strikingly parallel though increase in TH protein occurred later than the increase in the TH mRNA. These results suggest that in the rat LC: (1) there is a significant population of 'sleeping cells' in which TH expression is either inactivated or, at a low level of activation; (2) TH cellular concentration could exert a retrocontrol on its own expression in cells of the LC that contained TH and (3) TH expression appears to be regulated by different selective mechanisms in these two different subpopulations of noradrenergic cells within the LC.
Debure
L I
LI
Laboratoire de Neuropharmacologie Moléculaire, CERMEP, Lyon, France.
Moyse
E
E
Fevre-Montange
M
M
Hardin
H
H
Belin
M F
MF
Rousset
C
C
Pujol
J F
JF
Weissmann
D
D
D000818:Animals; D001345:Autoradiography; D001931:Brain Mapping / Q000379:methods; D002138:Calibration; D002452:Cell Count; D004525:Efferent Pathways / Q000502:physiology; D007150:Immunohistochemistry; D007274:Injections, Intraperitoneal; D008125:Locus Coeruleus / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors; D014446:Tyrosine 3-Monooxygenase / Q000032:analysis* / Q000096:biosynthesis / Q000235:genetics; D014749:Vincamine / Q000031:analogs & derivatives* / Q000494:pharmacology
D012333:RNA, Messenger; D014749:Vincamine; C044616:vindeburnol; D014446:Tyrosine 3-Monooxygenase