O-glycosidically linked oligosaccharides from peptidorhamnomannans of Sporothrix schenckii.
beta-Elimination of peptidorhamnomannans purified from yeast-like and mycelial phases of Sporothrix schenckii released neutral and acidic reduced oligosaccharides that were O linked to serine and/or threonine. Man-(alpha 1-2)Man-ol, Rha(alpha 1-3)Man(alpha 1-2)Man-ol, Rha(alpha 1-4)GlcA(alpha 1-2)Man(alpha 1-2)Man-ol, and Rha(alpha 1-4)[Rha(alpha 1-2)] GlcA(alpha 1-2)Man(alpha 1-2)Man-ol were characterized based on methylation analysis, proton magnetic resonance and fast atom bombardment mass spectrometry.
Depart. Biologia Celular e Genética-UERJ, Brasil.
L M
LM
Lopes-Alves
L
L
Mendonça-Previato
B
B
Fournet
P
P
Degand
J O
JO
Previato
D000596:Amino Acids / Q000032:analysis; D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000032:analysis; D002850:Chromatography, Gel; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification*; D009682:Magnetic Resonance Spectroscopy; D008351:Mannans / Q000737:chemistry*; D008745:Methylation; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry / Q000302:isolation & purification*; D013175:Sporothrix / Q000737:chemistry*
D000596:Amino Acids; D002241:Carbohydrates; D006027:Glycosides; D008351:Mannans; D009844:Oligosaccharides