In vivo binding and clearance of circulating antigen by bispecific heteropolymer-mediated binding to primate erythrocyte complement receptor.
We have used the avidin/biotin system to construct soluble, cross-linked bispecific heteropolymers containing mAb to both the primate E C receptor and the DNP group. These heteropolymers facilitate in vitro binding of DNP-bovine gamma-globulin (DNP-BGG) to both human and squirrel monkey E. Intravenous injection in squirrel monkeys of DNP-BGG followed by heteropolymer leads to E binding and clearance from the circulation of a significant fraction of both heteropolymer and DNP-BGG, without lysis or clearance of the E. This methodology may potentially be used to treat a variety of infectious diseases and other syndromes associated with blood-borne pathogens.
Department of Biochemistry, University of Virginia, School of Medicine, Charlottesville 22908.
R P
RP
Taylor
C J
CJ
Reist
W M
WM
Sutherland
A
A
Otto
R H
RH
Labuguen
E L
EL
Wright
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000941:Antigens / Q000378:metabolism*; D002417:Cattle; D004912:Erythrocytes / Q000276:immunology*; D006801:Humans; D008252:Macaca fascicularis; D008253:Macaca mulatta; D008657:Metabolic Clearance Rate; D011951:Receptors, Complement / Q000378:metabolism*; D017463:Receptors, Complement 3b; D012453:Saimiri; D005719:gamma-Globulins / Q000276:immunology
D000911:Antibodies, Monoclonal; D000941:Antigens; D011951:Receptors, Complement; D017463:Receptors, Complement 3b; D005719:gamma-Globulins