Comparative studies on the fermentative production of lantibiotics by staphylococci.
The production of the lanthionine-containing polypeptide antibiotics gallidermin from Staphylococcus gallinarum TU 3928 and pep 5 from S. epidermidis 5 is investigated with respect to regulation and stimulation of productivity by media components, optimization of both the media used and the fermentation process and is compared to the production of the lantibiotic epidermin from S. epidermidis TU 3298. Efficient methods for rapid quantification of lantibiotics, optimization of the media and a primary enrichment by adsorption chromatography are reported.
Lehrbereich Mikrobiologie/Antibiotika, Universität Tübingen, Federal Republic of Germany.
T
T
Hörner
V
V
Ungermann
H
H
Zähner
H P
HP
Fiedler
R
R
Utz
R
R
Kellner
G
G
Jung
D000409:Alanine / Q000031:analogs & derivatives / Q000378:metabolism; D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents; D001430:Bacteriocins; D002850:Chromatography, Gel; D003470:Culture Media; D005285:Fermentation / Q000502:physiology*; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D010452:Peptide Biosynthesis; D010455:Peptides; D010456:Peptides, Cyclic / Q000096:biosynthesis / Q000378:metabolism; D013210:Staphylococcus / Q000378:metabolism*; D013212:Staphylococcus epidermidis / Q000378:metabolism*; D013440:Sulfides
D000900:Anti-Bacterial Agents; D001430:Bacteriocins; D003470:Culture Media; D010455:Peptides; D010456:Peptides, Cyclic; D013440:Sulfides; C057656:gallidermin; C054222:lantibiotic Pep5; C050558:epidermin; C001520:lanthionine; D000409:Alanine