POU-specific domain of Oct-2 factor confers 'octamer' motif DNA binding specificity on heterologous Antennapedia homeodomain.
The bipartite DNA binding domain of the POU family of transcription factors contains a 'POU-specific' domain unique to this class of factors and a 'POU homeodomain' homologous to other homeodomains. We compared DNA binding of the Oct-2 factor POU domain and the Antennapedia (Antp) homeodomain with a chimeric Oct-2/Antp protein in which the distantly related Antp homeodomain was substituted for the Oct-2 POU homeodomain. The Oct-2/Antp chimeric protein bound both the octamer and the Antp sites efficiently, indicating that DNA binding specificity is contributed by both components of the POU domain.
Institute of Molecular Biology II, University of Zürich, Switzerland.
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Brugnera
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Xu
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Schaffner
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Arnosti
D051943:Antennapedia Homeodomain Protein; D001483:Base Sequence; D001665:Binding Sites; D004247:DNA / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D003850:Deoxyribonuclease I; D005801:Genes, Homeobox; D018398:Homeodomain Proteins; D008745:Methylation; D008969:Molecular Sequence Data; D009687:Nuclear Proteins; D050813:Octamer Transcription Factor-2; D050809:POU Domain Factors; D011485:Protein Binding; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors
D051943:Antennapedia Homeodomain Protein; D004268:DNA-Binding Proteins; D018398:Homeodomain Proteins; D009687:Nuclear Proteins; D050813:Octamer Transcription Factor-2; D050809:POU Domain Factors; C058435:POU2F2 protein, human; D011993:Recombinant Fusion Proteins; D014157:Transcription Factors; D004247:DNA; D003850:Deoxyribonuclease I