Fuzzy reasoning system for fault diagnosis of physiological activities in a cultivating process.
Aiming at development of a system which supports cultivating operations, a method to diagnose physiological activities in a cultivating process is presented, and a fuzzy expert system for diagnosing Lactobacillus casei cultivating process is implemented in this paper. This system can calculate specific rates of cell growth, substrate consumption, and product formation with measuring cell mass concentration, substrate concentration, and product concentration by using a turbidity sensor and HPLC. A database is implemented, where standard curves on specific rates representing characteristics of microorganisms are stored according to normalized substrate consumption. Comparing the calculated specific rates with standard values derived from the database, the system diagnoses physiological activities of the microorganisms. As a case study, a knowledge base for diagnosing lactic acid production process is implemented. The use of fault diagnosis on pH malfunctions by the expert system proves its reasonable performance.
Chemical Engineering Laboratory, RIKEN (The Institute of Physical and Chemical Research), Saitama, Japan.
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Bustamante
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Pokkinen
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Takuwa
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H
Asama
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Linko
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Endo
D001709:Biotechnology; D003470:Culture Media; D005103:Expert Systems; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D007780:Lacticaseibacillus casei / Q000254:growth & development*
D003470:Culture Media