Immunohistologic demonstration of myocardial proteins with applications.
The authors used antibodies specific for creatine kinase MB isoenzyme (CK-MB) and myosin light chain-1 (MLC-1) for immunohistologic staining. At appropriate dilutions of antibody, frozen sections of human heart muscle were positive for both CK-MB and MLC-1, whereas sections of human skeletal muscle were negative for both proteins. Staining for both CK-MB and MLC-1 also was demonstrated in an immature teratoma. Furthermore, staining was localized to the rhabdomyosarcomatous elements within the teratoma; other components of the tumor did not stain for CK-MB or MLC-1. Biopsies of skeletal muscle revealed that regenerative, but not intact normal or degenerating, fibers also contained CK-MB and MLC-1. Immunohistologic stains for CK-MB and MLC-1 may be useful as tumor markers and as markers for regenerative muscle fibers.
Clinical Pathology Department, Warren G. Magnuson Clinical Center, National Institutes of Health, Bethesda, Maryland 20892.
K
K
Emancipator
N
N
Urankar-Nagy
K A
KA
Leonard
G
G
Bradford
S N
SN
Emancipator
D001706:Biopsy; D003402:Creatine Kinase / Q000032:analysis; D006801:Humans; D007158:Immunologic Techniques; D007527:Isoenzymes; D009124:Muscle Proteins / Q000032:analysis*; D009132:Muscles / Q000737:chemistry / Q000473:pathology / Q000502:physiology; D009206:Myocardium / Q000737:chemistry*; D009218:Myosins / Q000032:analysis / Q000737:chemistry; D009363:Neoplasm Proteins / Q000032:analysis; D010982:Platelet-Derived Growth Factor / Q000032:analysis; D012038:Regeneration; D012208:Rhabdomyosarcoma / Q000737:chemistry; D013194:Staining and Labeling; D013724:Teratoma / Q000737:chemistry
D007527:Isoenzymes; D009124:Muscle Proteins; D009363:Neoplasm Proteins; D010982:Platelet-Derived Growth Factor; D003402:Creatine Kinase; D009218:Myosins