Use of a modified cupric acetate method for the detection and quantitation of xylanolytic activities: a comparative study with the congo red method.
A modified cupric acetate method for the screening and quantitation of xylanolytic activities was comparable with the more widely used congo red method with respect to sensitivity and ease of use and was shown to have points of merit over the latter. The use of a non-linear correction, in comparison to the conventional linear one, for the effect of dilution on the quantitative plate assay was evaluated.
Department of Microbiology, Panjab University, Chandigarh, India.
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N
Capalash
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P
Sharma
K G
KG
Gupta
D001407:Bacillus / Q000201:enzymology*; D003224:Congo Red; D003300:Copper; D006026:Glycoside Hydrolases / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D009942:Organometallic Compounds; D012988:Soil Microbiology; D013194:Staining and Labeling; D043325:Xylan Endo-1,3-beta-Xylosidase
D009942:Organometallic Compounds; C015092:cupric acetate; D003224:Congo Red; D003300:Copper; D006026:Glycoside Hydrolases; D043325:Xylan Endo-1,3-beta-Xylosidase