Peroxisomes in human colon carcinomas. A cytochemical and biochemical study.
The presence of peroxisomes and their enzymic content were investigated and compared in healthy and neoplastic human colon epithelial cells using cytochemical studies at the ultrastructural level as well as biochemical analyses. Catalase-positive organelles were found to be more numerous in normal than in colonic neoplastic cells. Biochemical assays revealed that no D-aminoacid oxidase or L-alpha-hydroxyacid oxidase activity was detected in normal or tumor tissues. The specific activities of catalase, fatty-acyl CoA oxidase and enoyl-CoA hydratase/3 hydroxyacyl-CoA dehydrogenase (the so-called peroxisomal bifunctional enzyme of the beta-oxidation system) were found to be diminished in carcinoma cells compared with the control tissue. The fall in catalase activity correlated well with tumor stage according to Dukes, suggesting that this peroxisomal enzyme could be used as a potential prognostic marker.
Laboratoire de Biologie Cellulaire du Développement-Université de Nancy I, Faculté des Sciences, Vandoeuvre-les-Nancy, France.
S
S
Cablé
J M
JM
Keller
S
S
Colin
K
K
Haffen
M
M
Kédinger
R M
RM
Parache
M
M
Dauça
D015094:3-Hydroxyacyl CoA Dehydrogenases / Q000032:analysis; D000230:Adenocarcinoma / Q000201:enzymology / Q000473:pathology / Q000648:ultrastructure*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000429:Alcohol Oxidoreductases / Q000032:analysis; D002374:Catalase / Q000032:analysis; D003110:Colonic Neoplasms / Q000201:enzymology / Q000473:pathology / Q000648:ultrastructure*; D003605:D-Amino-Acid Oxidase / Q000032:analysis; D004746:Enoyl-CoA Hydratase / Q000032:analysis; D006801:Humans; D007535:Isomerases / Q000032:analysis; D008830:Microbodies / Q000201:enzymology*; D008875:Middle Aged; D009097:Multienzyme Complexes / Q000032:analysis; D009363:Neoplasm Proteins / Q000032:analysis*; D009367:Neoplasm Staging; D063994:Peroxisomal Bifunctional Enzyme; D011379:Prognosis; D012004:Rectal Neoplasms / Q000201:enzymology / Q000473:pathology / Q000648:ultrastructure*
D009097:Multienzyme Complexes; D009363:Neoplasm Proteins; D000429:Alcohol Oxidoreductases; D015094:3-Hydroxyacyl CoA Dehydrogenases; C021380:L-2-hydroxyacid oxidase; D002374:Catalase; D003605:D-Amino-Acid Oxidase; C576253:EHHADH protein, human; D004746:Enoyl-CoA Hydratase; D063994:Peroxisomal Bifunctional Enzyme; D007535:Isomerases