Cloning and sequence analysis of cDNA encoding Rhizopus niveus lipase.
Complementary DNA encoding Rhizopus niveus lipase (RNL) was isolated from the R. niveus IF04759 cDNA library using a synthetic oligonucleotide corresponding to the amino acid sequence of the enzyme. A clone, which had an insert of 1.0 kilobase pairs, was found to contain the coding region of the enzyme. The lipase gene was expressed in Escherichia coli as a lacZ fusion protein. The mature RNL consisted of 297 amino acid residues with a molecular mass of 32 kDa. The RNL sequence showed significant overall homology to Rhizomucor miehei lipase and the putative active site residues were strictly conserved.
Kugimiya
W
W
Central Research Institute, Fuji Oil Co., Ltd., Ibaraki, Japan.
Otani
Y
Y
Kohno
M
M
Hashimoto
Y
Y
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004271:DNA, Fungal / Q000235:genetics*; D005656:Fungal Proteins / Q000235:genetics*; D015870:Gene Expression / Q000235:genetics; D008049:Lipase / Q000235:genetics*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009090:Mucorales / Q000201:enzymology / Q000235:genetics; D012233:Rhizopus / Q000201:enzymology* / Q000235:genetics
D004271:DNA, Fungal; D005656:Fungal Proteins; D008049:Lipase