Enhanced sensitivity to neoplastic transformation by 137Cs gamma-rays of cells in the G2-/M-phase age interval.
C3H mouse 10T1/2 cells, exposed to low doses of fission-spectrum neutrons, have an enhanced frequency of neoplastic transformation if protracted exposures are used (Hill et al. 1982, 1984a, 1985). To explain this anomaly, a biophysical model was proposed (Elkind 1991a,b) having the following essential features: (1) a narrow age interval exists in the growth cycle of 10T1/2 cells in which cells have high sensitivities to transformation; (2) in the latter age interval, cells are also sensitive to killing; (3) with increasing dose, cells at ages earlier in the growth cycle are progressively delayed from entering the sensitive age window; and (4) with increasing dose, the transformation sensitivity of cells in the sensitive window is not expressed due to increased killing. Protracted low doses result in elevated frequencies because of less killing, and reductions in delays in cell progression. Therefore, transformation-sensitive cells can progress into the sensitive interval to replace those that have progressed out of it. The unique shape and radiobiological properties of cells in and around mitosis, led to the proposal that the sensitive window is mitosis and possible cells just preceding or just following M phase (Elkind 1991a,b). Because of the likelihood that the properties of the cells in a sensitive window would not be evident only when fission-spectrum neutrons are used, this study was undertaken using 137Cs gamma-rays. We have found that late G2- to M-phase 10T1/2 cells have a maximal sensitivity to neoplastic transformation as well as to killing by 137Cs gamma-rays.
Department of Radiological Health Sciences, Colorado State University, Fort Collins 80523.
J
J
Cao
R L
RL
Wells
M M
MM
Elkind
D000818:Animals; D002470:Cell Survival / Q000528:radiation effects; D002471:Cell Transformation, Neoplastic / Q000528:radiation effects*; D002588:Cesium Radioisotopes; D004307:Dose-Response Relationship, Radiation; D005720:Gamma Rays; D007399:Interphase / Q000502:physiology*; D051379:Mice; D008809:Mice, Inbred C3H; D008938:Mitosis / Q000502:physiology*; D011836:Radiation Tolerance / Q000502:physiology*
D002588:Cesium Radioisotopes