Immunoassay of type IV collagenase/gelatinase (MMP-2) in human plasma.
We have developed a sensitive and specific sandwich type enzyme-linked immunosorbent assay (ELISA) to detect type IV collagenase (MMP-2) in human plasma which employs the combination of affinity purified rabbit polyclonal antibodies and mouse monoclonal antibodies to human MMP-2. The MMP-2 ELISA detects latent and activated MMP-2, MMP-2 complexed with TIMP and MMP-2 complexed with alpha 2 macroglobulin (65% efficiency). To determine whether physiologic conditions associated with increased connective tissue turnover are accompanied by increased MMP-2 levels in plasma, we compared enzyme levels in pregnant and nonpregnant women. Plasma MMP-2 (mean +/- standard deviation) was significantly increased (p less than 0.05) in the second half of pregnancy (650 +/- 312) as compared to early pregnancy (356 +/- 139) or the nonpregnant state (387 +/- 193). As a result of the linkage between type IV collagenase production by cancer cells and the metastatic phenotype, the assay of MMP-2 in plasma is of potential clinical value in cancer.
Department of Research, Department of Veterans Affairs Medical Center, Northport, NY 11768.
S
S
Zucker
R M
RM
Lysik
M
M
Gurfinkel
M H
MH
Zarrabi
W
W
Stetler-Stevenson
L A
LA
Liotta
H
H
Birkedal-Hansen
W
W
Mann
D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D003238:Connective Tissue / Q000378:metabolism; D004306:Dose-Response Relationship, Immunologic; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D006023:Glycoproteins / Q000502:physiology; D006801:Humans; D015151:Immunoblotting; D020780:Matrix Metalloproteinase 9; D003012:Microbial Collagenase / Q000037:antagonists & inhibitors / Q000097:blood*; D011247:Pregnancy; D012680:Sensitivity and Specificity; D019714:Tissue Inhibitor of Metalloproteinases; D000511:alpha-Macroglobulins / Q000502:physiology
D000911:Antibodies, Monoclonal; D006023:Glycoproteins; D019714:Tissue Inhibitor of Metalloproteinases; D000511:alpha-Macroglobulins; D003012:Microbial Collagenase; D020780:Matrix Metalloproteinase 9