Characterization of putative neurotransmitter N-acetyl-aspartyl-glutamic acid and some related compounds by fast atom bombardment and tandem mass spectrometry.
The characterization of the N-blocked neuropeptide N-acetyl-aspartyl-glutamic acid and of some related compounds was carried out by negative-ion fast atom bombardment mass spectrometry and collision-induced dissociation of the generated [M-H]- species. Thorough analysis of the fragment ion spectra allowed discrimination between sequence isomeric compounds, and highlighting of differences between dipeptides linked in the normal or iso mode.
Istituto di Tecnologie Biomediche Avanzate-CNR, Milano, Italy.
F M
FM
Rubino
L
L
Zecca
F
F
Chillemi
D000595:Amino Acid Sequence; D001224:Aspartic Acid / Q000032:analysis; D001923:Brain Chemistry; D004151:Dipeptides / Q000032:analysis*; D005971:Glutamates / Q000032:analysis; D018698:Glutamic Acid; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D018377:Neurotransmitter Agents / Q000032:analysis*; D016339:Spectrometry, Mass, Fast Atom Bombardment
D004151:Dipeptides; D005971:Glutamates; D018377:Neurotransmitter Agents; C027172:isospaglumic acid; D001224:Aspartic Acid; D018698:Glutamic Acid