[The effect of acute poisoning of rats with carbaryl on the transamination reactions involving alpha-ketoglutarate. Part I. Activity of plasma aminotransferases].
White male Wistar rats were poisoned orally with a single carbaryl dose 474 mg/kg (1/2 LD50). In the plasma of control animals and 2, 4, 24 and 72 hours after administration of the insecticide the activity of aminotransferase catalysing the reactions between alpha-ketoglutarate and six amino acids (cysteine, lysine, phenylalanine, leucine, asparagine and valine) was determined. Aminotransferases activity was expressed as the amount of glutamic acid developing during incubation of 1 cm3 of plasma for 1 hour. Glutamic acid was determined spectrophotometrically after chromatographic separation on paper. The results of the investigations demonstrated that acute intoxication with carbaryl caused a statistically significant increase in the activity of two aminotransferases in the plasma of experimental animals. A rise in the activity of the enzyme catalysing the reaction between alpha-ketoglutarate and asparagine ketoglutarate and lysine was observed.
Katedry i Zakładu Chemii Toksykologicznej Akademii Medycznej w Lublinie.
K
K
Jeleniewicz
S
S
Szczepaniak
H
H
Maciejewska-Kozak
D000818:Animals; D012721:Carbaryl / Q000506:poisoning*; D005970:Glutamate Synthase / Q000378:metabolism; D005971:Glutamates / Q000097:blood*; D018698:Glutamic Acid; D007656:Ketoglutaric Acids / Q000378:metabolism*; D008239:Lysine / Q000378:metabolism*; D008297:Male; D051381:Rats; D017208:Rats, Wistar
D005971:Glutamates; D007656:Ketoglutaric Acids; D018698:Glutamic Acid; D005970:Glutamate Synthase; D008239:Lysine; D012721:Carbaryl
Wpływ ostrego zatrucia szczurów karbarylem na rekcje transaminacji z udziałem alfa-ketoglutaranu. CZ. I. Aktywność aminotransferaz osoczowych.