Murine Thy-1+ dendritic epidermal T cell lines express granule-associated perforin and a family of granzyme molecules.
Two T cell receptor gamma/delta + murine dendritic epidermal T cell (DETC) lines with cytotoxic potential towards various tumor cell lines are shown to express perforin and granzyme A both at the mRNA and protein levels. Furthermore, mRNA transcripts for granzyme B and at least one of the other granzymes D, E, F and G are detected in amounts equivalent to a murine IL-2-dependent alpha/beta + cytotoxic T lymphocyte cell line. Hemolytic granules containing serine-esterase (granzyme A) activity are isolated from a DETC line. Thus, cytolytically-active Thy-1+ DETC lines contain the granule-associated pore-forming protein, perforin, and at least one member of each of the three subgroups of granzyme serine esterases (granzyme A, B and D/E/F/G). These data support the proposed role of gamma/delta + DETC in immune surveillance, possibly exerting cytolytic functions against virus- or parasite-infected, transformed or stressed cells.
Institute of Biochemistry, University of Lausanne, Epalinges, Switzerland.
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Krähenbühl
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Gattesco
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Tschopp
D000818:Animals; D000954:Antigens, Surface / Q000276:immunology*; D015152:Blotting, Northern; D002460:Cell Line; D003594:Cytoplasmic Granules / Q000378:metabolism; D003602:Cytotoxicity, Immunologic; D003713:Dendritic Cells / Q000276:immunology*; D015870:Gene Expression; D053804:Granzymes; D008562:Membrane Glycoproteins / Q000276:immunology*; D008565:Membrane Proteins / Q000378:metabolism*; D051379:Mice; D008815:Mice, Inbred Strains; D054353:Perforin; D052899:Pore Forming Cytotoxic Proteins; D012333:RNA, Messenger / Q000378:metabolism; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000276:immunology; D012697:Serine Endopeptidases / Q000378:metabolism*; D012867:Skin / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D018800:Thy-1 Antigens; D014407:Tumor Cells, Cultured
D000954:Antigens, Surface; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D052899:Pore Forming Cytotoxic Proteins; D012333:RNA, Messenger; D016692:Receptors, Antigen, T-Cell, gamma-delta; D018800:Thy-1 Antigens; D054353:Perforin; D053804:Granzymes; D012697:Serine Endopeptidases