Practical considerations in the measurement of culture fluorescence.
We have examined practical considerations associated with the use of a commercially available fluorescence probe for in situ measurements in bioreactors. The optical path length of the measurement was first determined and a flow cell subsequently designed. The environment (agitation/aeration rates) of the probe was found to have a significant influence on the measurement. These effects were eliminated by placing the probe in a recycle loop using the flow cell. Fluorescence measurement in the recycle loop was verified to be representative of the cell sample and to not affect cell metabolism.
Coppella
S J
SJ
Maryland Biotechnology Institute, University of Maryland Baltimore County 21228.
Rao
G
G
D004926:Escherichia coli / Q000166:cytology* / Q000378:metabolism; D005285:Fermentation; D005453:Fluorescence; D005456:Fluorescent Dyes; D009249:NADP / Q000378:metabolism
D005456:Fluorescent Dyes; D009249:NADP