The role of the IGF1 receptor in the regulation of cdc2 mRNA levels in fibroblasts.
The levels of cdc2 mRNA increase when quiescent cells are stimulated by growth factors. In BALB/c 3T3, both platelet-derived growth factor and insulin-like growth factor 1 (IGF-1) are required to increase cdc2 mRNA levels. In p6 cells, which constitutively overexpress the IGF-1 receptor, IGF-1 is sufficient. The importance of the IGF-1/IGF-1 receptor interaction in regulating the levels of cdc2 mRNA was further confirmed by showing that an antisense oligodeoxynucleotide to the IGF-1 receptor RNA inhibited the IGF-1-mediated increase.
Jefferson Cancer Institute, Thomas Jefferson University, Jefferson Medical College, Philadelphia, Pennsylvania 19107.
E
E
Surmacz
P
P
Nugent
Z
Z
Pietrzkowski
R
R
Baserga
D016475:3T3 Cells; D000818:Animals; D015152:Blotting, Northern; D016203:CDC2 Protein Kinase / Q000235:genetics*; D016895:Culture Media, Serum-Free; D005347:Fibroblasts / Q000378:metabolism; D005786:Gene Expression Regulation; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D010982:Platelet-Derived Growth Factor / Q000378:metabolism; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism*; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D017451:Receptors, Somatomedin; D014158:Transcription, Genetic; D014162:Transfection
D016895:Culture Media, Serum-Free; D010982:Platelet-Derived Growth Factor; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D017451:Receptors, Somatomedin; D007334:Insulin-Like Growth Factor I; D016203:CDC2 Protein Kinase; D012194:RNA-Directed DNA Polymerase