Cloning of a gene coding for phosphotransacetylase from Escherichia coli.
A phosphotransacetylase gene (pta) has been cloned from a genomic DNA library of Escherichia coli 1100, a derivative of strain K-12. The phosphotransacetylase activities of pta+ plasmid-containing strains were amplified about 150-fold under control of the lac promoter. The molecular weight of the phosphotransacetylase was estimated to be about 81,000 by sodium dodecyl sulphate-polyacrylamide gel electrophoresis. The pta gene was found to be downstream of ackA by a combination of restriction analysis and plasmid subcloning. It is located about 13 kb upstream of the purF-folC-hisT region of the chromosome.
Research and Development Division, Kikkoman Corporation, Chiba, Japan.
H
H
Yamamoto-Otake
A
A
Matsuyama
E
E
Nakano
D000084:Acetate Kinase / Q000096:biosynthesis / Q000235:genetics*; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005798:Genes, Bacterial; D009876:Operon; D010709:Phosphate Acetyltransferase / Q000096:biosynthesis / Q000235:genetics*; D015183:Restriction Mapping
D010709:Phosphate Acetyltransferase; D000084:Acetate Kinase