A yeast system for stable expression of hepatitis B surface antigen.
We have constructed a yeast strain that has integrated into its chromosomal ribosomal RNA gene site two copies of Hepatitis B virus surface (HBS) antigen gene under the control of the yeast (Saccharomyces cerevisiae) glyceraldehyde-3-phosphate dehydrogenase (GAP) promoter and terminator. The level of expression of HBS gene was low in the strain, but upon chemical and physical mutageneses, in combination with an immunological screening procedure, a mutant clone which expressed HBS protein at a high level was obtained. This mutant strain produces HBS antigen stably under non-selective conditions.
Shiosaki
K
K
Institute for Molecular and Cellular Biology, Osaka University, Japan.
Miyatsu
Y
Y
Takahara
K
K
Mizokami
H
H
Hamada
F
F
Matsubara
K
K
D002875:Chromosomes; D003001:Cloning, Molecular; D005814:Genes, Viral; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics; D006514:Hepatitis B Surface Antigens / Q000235:genetics* / Q000276:immunology; D016296:Mutagenesis; D010641:Phenotype; D010957:Plasmids; D011401:Promoter Regions, Genetic; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*
D006514:Hepatitis B Surface Antigens; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases