1H-and 13C-n.m.r. spectroscopy of 2-oxo-3-deoxy-D-glycero-D- galactononulosonic acid-containing oligosaccharide-alditols bearing Lewis X, Lewis Y and A-Lewis Y determinants isolated from the jelly coat of Pleurodeles waltl eggs.
Three acidic oligosaccharide-alditols carrying Lewis X, Lewis Y and A-Lewis Y determinants were isolated from the jelly coat of Pleurodeles waltl eggs. These compounds possess the following structures. Gal beta 1-4(Fuc alpha 1-3)GlcNAc beta 1-3[2-oxo-3-deoxy-D- glycero-D-galactononulosonic acid (KDN)alpha 2-6] GalNAc-ol; Fuc alpha 1-2Gal beta 1-4(Fuc alpha 1-3)GlcNAc beta 1-3(KDN alpha 2-6) GalNAc-ol and Fuc alpha 1-2(GalNAc alpha 1-3)Gal beta 1-4(Fuc alpha 1-3)GlcNAc beta 1-3(KDN alpha 2-6)GalNAc-ol. The complete 1H-n.m.r.-spectrum assignment for the three compounds and the 13C-n.m.r. analysis of the A-Lewis Y determinant-containing heptasaccharide are reported.
Strecker
G
G
Laboratoire de Chimie Biologique, Université des Sciences et Technologies de Lille, Villeneuve d'Ascq, France.
Wieruszeski
J M
JM
Michalski
J C
JC
Montreuil
J
J
D000818:Animals; D002240:Carbohydrate Sequence; D007983:Lewis Blood Group Antigens / Q000737:chemistry* / Q000302:isolation & purification; D016256:Lewis X Antigen / Q000737:chemistry* / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009077:Mucins / Q000737:chemistry; D009844:Oligosaccharides / Q000737:chemistry*; D009865:Oocytes / Q000737:chemistry*; D010999:Pleurodeles; D013400:Sugar Acids / Q000737:chemistry*; D013402:Sugar Alcohols / Q000737:chemistry*
D007983:Lewis Blood Group Antigens; D016256:Lewis X Antigen; D009077:Mucins; D009844:Oligosaccharides; D013400:Sugar Acids; D013402:Sugar Alcohols; C050008:3-deoxyglycero-galacto-nonulosonic acid