Transformation of Penicillium urticae with plasmids containing the hygromycin B resistance gene.
A transformation system with efficiencies between 6 and 50 stable transformants per micrograms of DNA was developed for Penicillium urticae J1 (ATCC48163) using hygromycin B-resistant plasmids containing or not containing fragments of the P. urticae genome. The tandem repeated integration and/or random integration of vector DNA were observed. Although P. urticae was unable to grow in the presence of 200 micrograms/ml hygromycin B, the transformants were resistant to more than 5 mg/ml of hygromycin B.
Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University, Nagano, Japan.
N
N
Kiuchi
A
A
Naruse
H
H
Yamamoto
J
J
Sekiguchi
D015139:Blotting, Southern; D002934:Cinnamates; D004247:DNA / Q000302:isolation & purification; D004352:Drug Resistance, Microbial / Q000235:genetics; D006921:Hygromycin B / Q000031:analogs & derivatives* / Q000494:pharmacology; D010407:Penicillium / Q000187:drug effects / Q000235:genetics*; D010957:Plasmids; D011523:Protoplasts; D014170:Transformation, Genetic
D002934:Cinnamates; D006921:Hygromycin B; C026273:hygromycin A; D004247:DNA