Sea anemone Radianthus macrodactylus--a new source of palytoxin.
A very potent non-protein toxin was isolated from the sea anemone Radianthus macrodactylus with the use of chromatography on polytetrafluoroethylene, CM-Sephadex C-25 and by cation and anion exchange HPLC. The toxin was identified as palytoxin by u.v.-, i.r.- and 500 MHz 1H NMR spectroscopy. Its LD50 was 0.74 +/- 0.29 micrograms/kg by i.v. injection into mice. So far, palytoxin has been associated with zoanthids only. The toxin caused the loss of haemoglobin from erythrocytes but only in about 2 hr after the beginning of incubation, which is characteristic for palytoxin from zoanthids. Sea anemone palytoxin was divided into major and minor components by HPLC. The latter proved to be a product of degradation of palytoxin.
Pacific Institute of Bioorganic Chemistry, Russian Academy of Sciences, Vladivostok.
V M
VM
Mahnir
E P
EP
Kozlovskaya
A I
AI
Kalinovsky
D000178:Acrylamides; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D002942:Circular Dichroism; D003064:Cnidarian Venoms / Q000032:analysis* / Q000302:isolation & purification / Q000633:toxicity; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism; D006454:Hemoglobins / Q000378:metabolism; D007928:Lethal Dose 50; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D051379:Mice; D012615:Sea Anemones / Q000737:chemistry*; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet; D000095702:Polyether Toxins
D000178:Acrylamides; D003064:Cnidarian Venoms; D006454:Hemoglobins; C010272:palytoxin; D000095702:Polyether Toxins