Toxicity of dimethyl sulfoxide as a solvent in bioassay system with HeLa cells evaluated colorimetrically with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide.
The toxicity of dimethyl sulfoxide (Me2SO) was examined in HeLa cells cultured at 37 degrees C for up to 72 hr. The growth of the cells was measured by a colorimetric method with the use of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), which gave good correlation between the cell number and the color development from the reduction of MTT under suitable conditions. When the initial number of cells was 3 x 10(4)/ml, Me2SO at 1% or less had no apparent effect on proliferation for up to 48 hr of incubation, but in longer incubations, cell growth was repressed. When the initial number of cells was 3 x 10(5)/ml, the effect of Me2SO was similar.
Ben Trivedi
A
A
Research Institute for Food Science, Kyoto University, Japan.
Kitabatake
N
N
Doi
E
E
D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects*; D003124:Colorimetry; D004396:Coloring Agents; D004121:Dimethyl Sulfoxide / Q000633:toxicity*; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D013778:Tetrazolium Salts; D013844:Thiazoles; D014407:Tumor Cells, Cultured
D004396:Coloring Agents; D013778:Tetrazolium Salts; D013844:Thiazoles; C022616:thiazolyl blue; D004121:Dimethyl Sulfoxide