An improved method for chromosome banding after fluorescence in situ hybridization: use of a tetramethylrhodhamine-conjugated BrdU antibody.
A method for high quality chromosome banding after in situ hybridization with biotinylated probes has been developed. Fluoresceine-conjugated avidin is used for probe detection, while chromosome banding is performed with a tetramethylrodhamine-conjugated anti-BrdU antibody. In this way probe localization and chromosome identification can be performed simultaneously simply by changing the incidental light wavelength.
Dipartimento di Genetica e Microbiologia, A. Buzzati-Traverso, Pavia, Italy.
A
A
De Sario
L
L
De Carli
E
E
Raimondi
D000328:Adult; D001973:Bromodeoxyuridine / Q000032:analysis* / Q000276:immunology; D002478:Cells, Cultured; D002503:Centromere / Q000648:ultrastructure; D002871:Chromosome Banding / Q000379:methods*; D004396:Coloring Agents; D003360:Cosmids; D016650:Fluorescein-5-isothiocyanate; D006801:Humans; D007621:Karyotyping; D008214:Lymphocytes / Q000166:cytology*; D008297:Male; D008856:Microscopy, Fluorescence / Q000379:methods; D009693:Nucleic Acid Hybridization; D012235:Rhodamines; D014998:Y Chromosome
D004396:Coloring Agents; D012235:Rhodamines; C005358:tetramethylrhodamine; D001973:Bromodeoxyuridine; D016650:Fluorescein-5-isothiocyanate