A 6 h microslide immunodiffusion assay for confirmed detection of staphylococcal enterotoxins.
Use of appropriately balanced immunological reagents and incubation of microslides at 45 degrees C resulted in confirmed assay results in 6 h for staphylococcal enterotoxins. The sensitivity of the 45 degrees C-6 h assay was comparable to the 37 degrees C-24 h assay: 0.1 micrograms of staphylococcal enterotoxin A/ml.
Department of Food Science and Nutrition, University of Minnesota, Saint Paul 55108.
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McLandsborough
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Tatini
D004768:Enterotoxins / Q000032:analysis*; D005516:Food Microbiology; D005779:Immunodiffusion; D011237:Predictive Value of Tests; D013211:Staphylococcus aureus; D013997:Time Factors
D004768:Enterotoxins