Antibiotic sensitivity testing of bacteria by microcolony inhibition and image analysis.
A rapid method for determining the sensitivity of Staphylococcus aureus to beta-lactams is described. The method involves the culture of microcolonies of the bacterium on the surface of antibiotic-containing agar medium on microscope slides. The areas of the microcolonies formed are measured by means of a microscope and image analyzer. The sensitivity of the bacterium can be estimated in 4 h by this technique and the results are comparable to those obtained by overnight incubation.
Hammonds
S J
SJ
Department of Life Sciences, Trent Polytechnic, Nottingham, UK.
Adenwala
F
F
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002506:Cephalexin / Q000494:pharmacology; D007091:Image Processing, Computer-Assisted; D008712:Methicillin / Q000494:pharmacology; D008826:Microbial Sensitivity Tests / Q000379:methods*; D010406:Penicillins / Q000494:pharmacology; D013211:Staphylococcus aureus / Q000187:drug effects*
D000900:Anti-Bacterial Agents; D010406:Penicillins; D002506:Cephalexin; D008712:Methicillin