Ontogeny of the distribution and colocalization of calbindin D28K within neural and endocrine cells of the gastrointestinal tract of fetal and neonatal sheep.
Using immunocytochemical techniques we have demonstrated that Calbindin D28K (CaBP) is present in the gastrointestinal tract of ovine fetuses early in development (by day 45). At day 45, CaBP was limited to neuronal elements in the developing intestine. By day 100, CaBP immunoreactivity was abundant in both epithelial endocrine cells and nerves of the submucous and myenteric ganglia. The location of CaBP containing cells and fibers was similar in duodenal sections taken from day 100 and term (145 days), as well as those taken from 24-48 h postnatal lambs. CaBP is colocalized in endocrine cells containing gastrin, glucagon, somatostatin and neurotensin, but not glucose dependent insulinotrophic peptide (GIP). Furthermore, it is extensively colocalized in nerve fibers and cells containing neurotensin but not somatostatin or vasoactive intestinal peptide. The colocalization of CaBP within various endocrine and nerve cells does not change in fetal sheep over the last one-third of gestation and there is no difference between fetal and neonatal sheep.
Department of Obstetrics and Gynecology, University of Western Ontario, London, Canada.
P E
PE
Houghton
A M
AM
Buchan
J R
JR
Challis
D000818:Animals; D064092:Calbindin 1; D064026:Calbindins; D004064:Digestive System / Q000737:chemistry* / Q000166:cytology / Q000254:growth & development; D004702:Endocrine Glands / Q000737:chemistry*; D005260:Female; D005333:Fetus; D006801:Humans; D007150:Immunohistochemistry; D007231:Infant, Newborn; D008297:Male; D009474:Neurons / Q000737:chemistry*; D018377:Neurotransmitter Agents / Q000032:analysis; D011247:Pregnancy; D064030:S100 Calcium Binding Protein G / Q000032:analysis* / Q000502:physiology; D012756:Sheep; D014018:Tissue Distribution
C576498:CALB1 protein, human; D064092:Calbindin 1; D064026:Calbindins; D018377:Neurotransmitter Agents; D064030:S100 Calcium Binding Protein G