Biologic activities of HIV-1 envelope glycoprotein: the effects of crosslinking.
We have examined the biologic activities of native and recombinant preparations of human immunodeficiency virus envelope glycoprotein (gp120), both derived from the HIV-1B strain. Antibody to gp120 was used to evaluate the effects of crosslinking gp120 on signalling by the CD4 receptor. Our results indicate that native and recombinant gp120 produce identical effects in our assay systems. Crosslinking gp120 amplified its chemoattractant activity for lymphocytes and monocytes and increased the peak intracellular calcium level, compared with binding of gp120 alone. The induction of inositol trisphosphate (IP3) production, induction of interleukin 2 receptors (IL2R), and inhibition of lymphocyte proliferation following treatment with gp120 were not enhanced by the addition of crosslinking antibody.
Pulmonary Center, Boston University School of Medicine, Conte Center, MA 02118-2394, USA.
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Cruikshank
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Center
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Pyle
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Kornfeld
D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects / Q000276:immunology; D002118:Calcium / Q000032:analysis / Q000502:physiology; D002478:Cells, Cultured; D002633:Chemotaxis / Q000187:drug effects / Q000502:physiology; D015699:HIV Envelope Protein gp120 / Q000494:pharmacology / Q000502:physiology*; D015497:HIV-1 / Q000737:chemistry; D006801:Humans; D007295:Inositol Phosphates / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects / Q000502:physiology; D018123:Receptors, Interleukin / Q000187:drug effects; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology
D015699:HIV Envelope Protein gp120; D007295:Inositol Phosphates; D018123:Receptors, Interleukin; D002118:Calcium