The in vivo depletion of CD4+ T cells prevents antigen-induced eosinophil infiltration into mouse skin.
In order to determine the role of CD4+ T cells and CD8+ T cells in causing antigen-induced eosinophil infiltration into the site of late-phase reaction (LPR), we examined the effect of the in vivo depletion of CD4+ T cells and CD8+ T cells on antigen-induced eosinophil infiltration into mouse skin. Eosinophil infiltration into the skin of ovalbumin (OA)-sensitized BALB/c mice was biphasic after subcutaneous OA challenge, reaching the first peak at 6 h and the second peak at 24 to 48 h. The in vivo depletion of CD4+ T cells by pretreatment with anti-L3T4 monoclonal antibody (mAb) significantly decreased the second peak (at 24 h and 48 h), but not the first peak (at 6 h), of OA-induced eosinophil infiltration into the skin of OA-sensitized mice. However, the depletion of CD8+ T cells by pretreatment with anti-Lyt-2 mAb had no significant effect on either the first or second peak of OA-induced cutaneous eosinophilia in the mouse. These results indicate that CD4+ T cells, but not CD8+ T cells, play an important role in causing antigen-induced eosinophil recruitment of cutaneous LPR.
Tomoe
S
S
Second Department of Internal Medicine, Chiba University School of Medicine.
Iwamoto
I
I
Yoshida
S
S
Tomioka
H
H
D000818:Animals; D000941:Antigens / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000502:physiology*; D004804:Eosinophils / Q000276:immunology*; D006968:Hypersensitivity, Delayed / Q000276:immunology; D008212:Lymphocyte Depletion; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000276:immunology; D012867:Skin / Q000276:immunology*
D000941:Antigens; D010047:Ovalbumin