Multiple rpoD-related genes of cyanobacteria.
Genomes of many eubacterial strains have been shown to encode for multiple rpoD-related genes. In this report, we describe the identification of the multiple rpoD-related genes of cyanobacterial strains. DNAs of three cyanobacterial strains, Anabaena sp. PCC7120, Synechococcus sp. PCC7942, and Synechocystis sp. PCC6803, were examined by Southern hybridization, using a synthetic probe designed for detecting rpoD or rpoD-related genes. Four or five hybridization signals were found in each DNA. Four DNA regions of Synechococcus sp. PCC7942 corresponding to the hybridization signals were cloned and partially sequenced. The sequence data indicate the presence of genes, named rpoD1, rpoD2, rpoD3, and rpoD4, whose products are highly similar to the basic structure of the principal sigma factors of eubacterial strains. The rpoD1 gene showed the greatest similarity to the sigA gene of Anabaena sp. PCC7120.
Tanaka
K
K
Institute of Applied Microbiology, University of Tokyo, Japan.
Masuda
S
S
Takahashi
H
H
D000595:Amino Acid Sequence; D017033:Anabaena / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D001709:Biotechnology; D000458:Cyanobacteria / Q000235:genetics*; D015342:DNA Probes; D004269:DNA, Bacterial / Q000235:genetics; D012321:DNA-Directed RNA Polymerases; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D005810:Multigene Family; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D012808:Sigma Factor / Q000235:genetics
D001426:Bacterial Proteins; D015342:DNA Probes; D004269:DNA, Bacterial; D012808:Sigma Factor; C081775:RNA polymerase sigma 70; D012321:DNA-Directed RNA Polymerases