Cloning and expression of alpha-amylase from Bacillus amyloliquefaciens in a stable plasmid vector in Lactobacillus plantarum.
Lactobacillus plantarum is used in a wide range of agricultural and food fermentations. In this paper we report the introduction of alpha-amylase into the organism from Bacillus amyloliquefaciens on a stable recombinant plasmid. The genetically manipulated organism grew on MRSB medium supplemented with starch and it may be a prototype for the development of lactobacilli able to use an increased range of substrates in commercial fermentations.
Biotechnology Centre, Cranfield Institute of Technology, Bedford, UK.
S
S
Jones
P J
PJ
Warner
D001407:Bacillus / Q000201:enzymology* / Q000235:genetics; D015139:Blotting, Southern; D003001:Cloning, Molecular; D015342:DNA Probes; D004269:DNA, Bacterial / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D015971:Gene Expression Regulation, Enzymologic; D005822:Genetic Vectors; D007778:Lactobacillus / Q000201:enzymology* / Q000235:genetics; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D000516:alpha-Amylases / Q000235:genetics*
D015342:DNA Probes; D004269:DNA, Bacterial; D000516:alpha-Amylases