Amplification fragment length polymorphism in Brucella strains by use of polymerase chain reaction with arbitrary primers.
DNA heterogeneity among members of the genus Brucella was demonstrated with the arbitrarily primed polymerase chain reaction (AP-PCR). Simple, reproducible genomic fingerprints from DNA of 25 different Brucella strains were generated with five arbitrarily chosen primers, alone and in pairs, with the PCR. Reaction conditions were optimized for each primer. Several DNA segments were amplified in each sample with all of the primers. PCR products that are not shared among all strains act as polymorphic markers. Polymorphism was apparent for each primer. The Brucella strains can be distinguished according to the banding patterns of their amplified DNA on agarose gels, and the differences can be diagnostic of specific strains. To determine genetic relatedness among the Brucella strains, similarity coefficients were calculated. Statistical analysis of the similarity coefficients revealed the degrees of relatedness among strains of the genus Brucella.
USDA/ARS/NADC, Ames, Iowa 50010.
A
A
Fekete
J A
JA
Bantle
S M
SM
Halling
R W
RW
Stich
D001483:Base Sequence; D002002:Brucella / Q000145:classification / Q000235:genetics*; D016172:DNA Fingerprinting; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D013223:Statistics as Topic