Cell cycle-dependent changes in tissue transglutaminase mRNA levels in bovine endothelial cells.
The pattern of transglutaminase gene expression through the cell cycle was examined by Northern blot analysis using cultured bovine endothelial cells and a cDNA probe. When the cells reached confluency or were arrested in G0/G1 phase by nutrition deprivation, transglutaminase mRNA rose to a very high level; S- and M-phase extracts showed high and low levels, respectively. Subcellular localization studies by sucrose gradient centrifugation and immunostaining demonstrated that the majority of transglutaminase is present in cytosols throughout the cycle. The cell cycle-dependent changes in the transglutaminase mRNA levels strongly support the implicated involvement of the enzyme in cell growth, differentiation, and senescence.
Department of Biological Sciences, Tokyo Institute of Technology, Japan.
K
K
Nara
Y
Y
Aoyama
T
T
Iwata
H
H
Hagiwara
S
S
Hirose
D000818:Animals; D016590:Aphidicolin / Q000494:pharmacology; D015152:Blotting, Northern; D002417:Cattle; D002453:Cell Cycle / Q000502:physiology*; D002467:Cell Nucleus / Q000201:enzymology; D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient; D015342:DNA Probes; D004730:Endothelium, Vascular / Q000201:enzymology*; D015870:Gene Expression; D007399:Interphase; D008938:Mitosis; D012333:RNA, Messenger / Q000378:metabolism*; D016196:S Phase; D011503:Transglutaminases / Q000235:genetics*
D015342:DNA Probes; D012333:RNA, Messenger; D016590:Aphidicolin; D011503:Transglutaminases