[A study to clarify the mechanism of the usefulness of the macrolides--the influence of clarithromycin to biofilm with P. aeruginosa].
Clarithromycin (CAM) was administered long-term to patients with diffuse panbronchiolitis (DPB) to clarify the mechanism of the usefulness of the macrolides (MLs). 1. A tendency for clinical improvement was observed in 17 patients with DPB. Bacteria were eradicated in 7 of 9 patients with P. aeruginosa found in sputum. 2. A biofilm experimental model with P. aeruginosa was found to be destructed through constant contact with CAM and formed into a single cell with a smooth surface. 3. It was believed that the new lesion forming capability of P. aeruginosa that had been in contact with CAM was reduced due to a significant decrease in adherence to tissue. P. aeruginosa was principally eradicated by the host factors. These results suggested that the improvement in the prognosis of DPB with P. aeruginosa in the sputum after adding MLs was closely related to the destructive effect of the MLs on the biofilm.
First Department of Internal Medicine, School of Medicine, Kyorin University.
H
H
Takeda
N
N
Oogaki
N
N
Kikuchi
H
H
Kobayashi
T
T
Akashi
D000818:Animals; D001988:Bronchiolitis / Q000188:drug therapy*; D017291:Clarithromycin / Q000494:pharmacology*; D003102:Colloids; D006801:Humans; D066298:In Vitro Techniques; D051379:Mice; D008855:Microscopy, Electron, Scanning; D011091:Polyesters; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000648:ultrastructure
D003102:Colloids; D011091:Polyesters; D017291:Clarithromycin