Monoamines and their metabolites in the amphibian (Ambystoma tigrinum) brain: quantitative changes during metamorphosis and captivity.
1. Monoamine neurotransmitters (epinephrine, norepinephrine, dopamine, serotonin and some of their metabolites (DOPEG, MHPG, DOPAC, 5-HIAA) were measured by HPLC in extracts from telencephalon (TEL) and diencephalon-midbrain (DM) before, during at the end of metamorphosis. 2. During metamorphosis MHPG increased and 5-HIAA decreased in TEL and DM while DOPEG decreased only in DM. 3. Monoamine levels were greater in the TEL and a larger increase in MHPG occurred there. 4. Captivity without metamorphosis also caused a significant depression of 5-HIAA in TEL and depression of DOPEG, MHPG and DOPAC in DM.
Department of Environmental, Population and Organismic Biology, University of Colorado, Boulder 80309-0334.
D O
DO
Norris
J A
JA
Carr
P H
PH
Desan
T K
TK
Smock
M F
MF
Norman
D000557:Ambystoma / Q000378:metabolism / Q000502:physiology*; D000818:Animals; D015306:Biogenic Monoamines / Q000378:metabolism*; D001835:Body Weight / Q000502:physiology; D001923:Brain Chemistry / Q000502:physiology*; D002851:Chromatography, High Pressure Liquid; D004027:Diencephalon / Q000502:physiology; D008636:Mesencephalon / Q000378:metabolism; D008675:Metamorphosis, Biological / Q000502:physiology*; D009929:Organ Size / Q000502:physiology; D013687:Telencephalon / Q000378:metabolism
D015306:Biogenic Monoamines