[Kinetics of merthiolate-induced aggregation of human platelets].
Incubation of human platelets (in the form of platelet rich plasma or washed platelet suspension) with sodium merthiolate (ethyl mercuric salicylate inhibiting the arachidonic acid incorporation into phospholipids) induces their irreversible aggregation, which is accompanied by TxB2 synthesis. The merthiolate-induced aggregation has a lag-period of 0.5-10 min, whose magnitude is inversely correlated with the merthiolate concentration. The concentration dependencies of the rate of the merthiolate-induced and arachidonate-induced aggregation are threshold ones; the Hill coefficients are more than 30. The merthiolate-induced aggregation occurs in two phases: a slow phase which is independent of the arachidonic acid cyclooxygenase metabolism and a fast phase which is fully blocked by indomethacin. This aggregation is inhibited by PGE1 and ajoene (an inhibitor of the fibrinogen interaction with the fibrinogen receptor, GPIIb/IIIa). Quantitative and qualitative analyses of the experimental data were performed, using a model which took account of: (a) increase in the concentration of free endogenous arachidonic acid resulting from the inhibition by merthiolate of the arachidonic acid re-incorporation into phospholipids, and (b) existence of a threshold intracellular arachidonic acid concentration needed for the irreversible aggregation of platelets.
P V
PV
Vrzheshch
A V
AV
Tatarintsev
E V
EV
Orlova
D E
DE
Ershov
S D
SD
Varfolomeev
D000527:Alprostadil / Q000494:pharmacology; D016718:Arachidonic Acid / Q000378:metabolism; D004220:Disulfides / Q000494:pharmacology; D006801:Humans; D007700:Kinetics; D010936:Plant Extracts / Q000494:pharmacology; D010974:Platelet Aggregation / Q000187:drug effects*; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology; D013454:Sulfoxides; D013849:Thimerosal / Q000494:pharmacology*; D013931:Thromboxanes / Q000096:biosynthesis
D004220:Disulfides; D010936:Plant Extracts; D010975:Platelet Aggregation Inhibitors; D013454:Sulfoxides; D013931:Thromboxanes; D013849:Thimerosal; D016718:Arachidonic Acid; C048980:ajoene; D000527:Alprostadil
Kinetika mertioliat-indutsirovannoĭ agregatsii trombotsitov cheloveka.