Immunization with Porphyromonas (Bacteroides) gingivalis fimbriae protects against periodontal destruction.
Adhesive fimbriae from Porphyromonas gingivalis are cell surface structures which may be important in the virulence of this oral pathogen and thus may serve as a critical or target antigen. Immunization with highly purified 43-kDa fimbrial protein protected against periodontal tissue destruction when tested in the P. gingivalis-infected gnotobiotic rat model. A similarly highly purified 75-kDa cell surface component did not provide protection. Heat-killed whole-cell and sonicated cell surface extracts which contain the 43-kDa protein as well as the 75-kDa component were protective also. This study indicates that the fimbrial protein may serve as a model for the development of effective vaccines against periodontitis, a major human oral disease.
Evans
R T
RT
Department of Oral Biology, State University of New York, Buffalo 14214.
Klausen
B
B
Sojar
H T
HT
Bedi
G S
GS
Sfintescu
C
C
Ramamurthy
N S
NS
Golub
L M
LM
Genco
R J
RJ
D016301:Alveolar Bone Loss; D000704:Analysis of Variance; D000818:Animals; D000917:Antibody Formation; D000942:Antigens, Bacterial / Q000276:immunology; D000954:Antigens, Surface / Q000276:immunology; D001442:Bacteroides Infections / Q000517:prevention & control*; D002401:Cathepsin B / Q000032:analysis; D056668:Cathepsin L; D002403:Cathepsins / Q000032:analysis; D003546:Cysteine Endopeptidases; D010450:Endopeptidases; D010861:Fimbriae, Bacterial / Q000276:immunology*; D018093:Gelatinases; D005856:Germ-Free Life; D005881:Gingiva / Q000201:enzymology; D003012:Microbial Collagenase / Q000032:analysis; D010434:Pepsin A / Q000032:analysis; D010510:Periodontal Diseases / Q000473:pathology / Q000517:prevention & control*; D016966:Porphyromonas gingivalis; D051381:Rats; D011919:Rats, Inbred Strains; D015164:Vaccines, Inactivated / Q000276:immunology
D000942:Antigens, Bacterial; D000954:Antigens, Surface; D015164:Vaccines, Inactivated; D002403:Cathepsins; D010450:Endopeptidases; D003546:Cysteine Endopeptidases; D002401:Cathepsin B; C534281:CTSL protein, human; D056668:Cathepsin L; C534280:Ctsl protein, rat; D010434:Pepsin A; D018093:Gelatinases; D003012:Microbial Collagenase