A new method for protein crystallization using high pressure.
Crystallization methods for proteins have been the subject of decades of development yet protein crystallization remains the limiting step in structural studies. We present here a new method for protein crystallization--based on the use of high pressure--that enabled us to accelerate dramatically the growth of glucose isomerase crystals. We think this method may have a more general utility.
Visuri
K
K
Cultor Ltd., Technology Center, Kantvik, Finland.
Kaipainen
E
E
Kivimäki
J
J
Niemi
H
H
Leisola
M
M
Palosaari
S
S
D019747:Aldose-Ketose Isomerases; D002238:Carbohydrate Epimerases / Q000096:biosynthesis / Q000235:genetics; D003460:Crystallization; D011506:Proteins; D014961:X-Ray Diffraction
D011506:Proteins; D002238:Carbohydrate Epimerases; D019747:Aldose-Ketose Isomerases; C023298:xylose isomerase