An investigation of Japanese subjects maps susceptibility to type 1 (insulin-dependent) diabetes mellitus close to the DQA1 gene.
Insulin-dependent diabetic and control subjects of Japanese origin were HLA-DRB1, -DQB1, and -DQA1 typed using restriction fragment length polymorphism analysis and sequence-specific oligonucleotide gene probing. The DQA1 allele DQA1*0301 was positively associated with the disease [48/52 (92%) diabetic patients versus 44/64 (69%) control subjects, Pc less than 0.03, RR = 4.97]. Alleles of the DRB1 and DQB1 genes showed no significant association with the disease. The frequency of DQB1 genotypes encoding the amino acid aspartic acid at position 57 of the DQ beta chain did not differ significantly between subjects with insulin-dependent diabetes mellitus (IDDM) and controls. These findings suggest that a susceptibility allele for IDDM in the Japanese is more closely associated with the DQA1 gene than the DQB1 gene.
Department of Medicine, University of Birmingham, England.
K H
KH
Jacobs
D
D
Jenkins
C
C
Mijovic
M
M
Penny
Y
Y
Uchigata
D
D
Cavan
Y
Y
Hirata
T
T
Otani
J
J
Fletcher
A H
AH
Barnett
D000483:Alleles; D001483:Base Sequence; D002874:Chromosome Mapping; D003922:Diabetes Mellitus, Type 1 / Q000453:epidemiology / Q000235:genetics*; D004198:Disease Susceptibility; D005787:Gene Frequency; D006683:HLA-DQ Antigens / Q000235:genetics*; D059848:HLA-DQ alpha-Chains; D059866:HLA-DQ beta-Chains; D006801:Humans; D007564:Japan; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D012150:Polymorphism, Restriction Fragment Length
D006683:HLA-DQ Antigens; D059848:HLA-DQ alpha-Chains; D059866:HLA-DQ beta-Chains; C069050:HLA-DQA1 antigen; C069051:HLA-DQB1 antigen; D015345:Oligonucleotide Probes