Molecular interaction between HIV-1 major envelope glycoprotein and dextran sulfate.
We investigated at the molecular level the interaction between, HIV-1 recombinant gp160 (rgp160) and low-molecular-weight dextran sulfate. We demonstrate the occurrence of a specific interaction between rgp160 and sulfated dextran beads, which is saturable, pH-dependent and inhibitable by soluble dextran sulfate but not by soluble dextran. This specific interaction has a low affinity, with an estimated Kd in the 10(-4) M range. In addition, the binding of rgp160 to soluble recombinant CD4 (sT4) can only be inhibited by the preincubation of rgp160, but not of sT4, with dextran sulfate. Taken together, these results demonstrate the occurrence of a low affinity, but specific interaction between dextran sulfate and rgp160. This may account, at least in part, for the anti-HIV-1 activity of dextran sulfate.
Laboratoire de Biologie Cellulaire, Faculté de Médecine, Bobigny, France.
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E
Mbemba
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V
Chams
J C
JC
Gluckman
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D
Klatzmann
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L
Gattegno
D015704:CD4 Antigens / Q000378:metabolism; D002846:Chromatography, Affinity; D016264:Dextran Sulfate / Q000494:pharmacology*; D003911:Dextrans; D004347:Drug Interactions; D015686:Gene Products, env / Q000378:metabolism*; D019302:HIV Envelope Protein gp160; D015497:HIV-1 / Q000187:drug effects*; D011498:Protein Precursors / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D014759:Viral Envelope Proteins / Q000378:metabolism*
D015704:CD4 Antigens; D003911:Dextrans; D015686:Gene Products, env; D019302:HIV Envelope Protein gp160; D011498:Protein Precursors; D011994:Recombinant Proteins; D014759:Viral Envelope Proteins; C052839:Cytodex; D016264:Dextran Sulfate