Expression of platelet-derived growth factor and its receptors in proliferative disorders of fibroblastic origin.
Platelet-derived growth factor (PDGF) is known to stimulate the proliferation of connective tissue-derived cells in vitro. Less is known about its functions in vivo, and the role of PDGF in the development of human tumors has not been clarified. The authors have investigated the occurrence of PDGF and PDGF receptors in a series of proliferative disorders of fibroblastic origin using immunohistochemical and in situ hybridization techniques. High expression of PDGF beta-receptor mRNA and protein was found in the malignant tumors, and also in some benign lesions, such as dermatofibroma. In all these cases, benign as well as malignant, the PDGF B-chain mRNA, and less clearly, the PDGF A-chain mRNA, were coexpressed with the beta-receptor. In contrast, high expression of PDGF alpha-receptor mRNA was only found in fully malignant lesions, i.e., malignant fibrous histiocytoma. These data indicate that an autocrine growth stimulation via the PDGF beta-receptor could occur in an early phase of tumorigenesis, and may be a necessary but insufficient event for the progression into fully malignant human connective tissue lesions.
Smits
A
A
Ludwig Institute for Cancer Research, Biomedical Center, Uppsala, Sweden.
Funa
K
K
Vassbotn
F S
FS
Beausang-Linder
M
M
af Ekenstam
F
F
Heldin
C H
CH
Westermark
B
B
Nistér
M
M
D000423:Alcian Blue; D000918:Antibody Specificity; D005347:Fibroblasts / Q000502:physiology; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D009369:Neoplasms / Q000378:metabolism*; D009693:Nucleic Acid Hybridization; D010982:Platelet-Derived Growth Factor / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000378:metabolism*; D017479:Receptors, Platelet-Derived Growth Factor; D012871:Skin Diseases / Q000378:metabolism*; D013194:Staining and Labeling
D010982:Platelet-Derived Growth Factor; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D017479:Receptors, Platelet-Derived Growth Factor; D000423:Alcian Blue