Multiple TCR V beta usage by infiltrates of young NOD mouse islets of Langerhans. A polymerase chain reaction analysis.
Because a restricted repertoire of T-cell receptor (TCR) V beta gene expression has been reported in other autoimmune diseases, the possibility of similarly restricted V beta gene expression by T-cell infiltrates of NOD mouse islets was examined. With isolated islets from 4- to 12-wk-old NOD mice, a prospective polymerase chain reaction analysis with 18 V beta-specific oligonucleotide primers was performed on the noncloned and unexpanded islet-infiltrating T cells. The methodology used permitted the detection of a minimum of 50 T cells. In contrast to the restricted TCR V beta gene usage reported for other autoimmune diseases, infiltrates of even the youngest mice were characterized by expression of multiple V beta gene segments.
Waters
S H
SH
Department of Pathology, University of Massachusetts Medical School, Worcester.
O'Neil
J J
JJ
Melican
D T
DT
Appel
M C
MC
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D005260:Female; D015870:Gene Expression; D007515:Islets of Langerhans / Q000276:immunology*; D046911:Macromolecular Substances; D008297:Male; D051379:Mice; D016688:Mice, Inbred NOD; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D016133:Polymerase Chain Reaction / Q000379:methods; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012194:RNA-Directed DNA Polymerase; D011948:Receptors, Antigen, T-Cell / Q000235:genetics*
D046911:Macromolecular Substances; D009838:Oligodeoxyribonucleotides; D011948:Receptors, Antigen, T-Cell; D012313:RNA; D012194:RNA-Directed DNA Polymerase