Monoclonal antibody GOM-2 binds to blood group B-Le(y) active glycolipid antigens on human gastric cancer cells, KATO-III.
The antigen structure of a mouse monoclonal antibody, GOM-2, established by immunization with KATO-III human gastric cancer cells, was examined. GOM-2 reactive glycolipids were prepared from KATO-III cells and treated with endoglycoceramidase. Structural studies of ten GOM-2 reactive oligosaccharides by a combination of glycosidase digestions, methylation, and affinity chromatography on an Ulex europeus agglutinin I (UEA-I) column revealed that nine of them had a Y-related B-active difucosylated determinant (B-Le(y)) and one had a B-active determinant. Affinity chromatography of the purified and modified oligosaccharides on an immobilized GOM-2 column demonstrated that GOM-2 has a novel binding specificity: it binds tightly to the biantennary structure carrying the B-Le(y) determinant at the termini or the branched structure carrying the B-Le(y) structure at two nonreducing termini.
Formulation Research Institute, Otsuka Pharmaceutical Company, Tokushima, Japan.
S
S
Sueyoshi
H
H
Nagakura
A
A
Kato
S
S
Uetsuki
Y
Y
Nakayama
M
M
Adachi
D000911:Antibodies, Monoclonal / Q000276:immunology*; D001666:Binding Sites, Antibody; D002240:Carbohydrate Sequence; D006017:Glycolipids / Q000276:immunology*; D006801:Humans; D007983:Lewis Blood Group Antigens / Q000276:immunology*; D008563:Membrane Lipids / Q000276:immunology; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000276:immunology; D013274:Stomach Neoplasms / Q000276:immunology*; D013402:Sugar Alcohols / Q000737:chemistry; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D006017:Glycolipids; D007983:Lewis Blood Group Antigens; D008563:Membrane Lipids; D009844:Oligosaccharides; D013402:Sugar Alcohols