Structure and expression of human and rat D2 dopamine receptor genes.
D2 dopamine receptor may be related with the pathogenesis of Parkinson's disease and schizophrenia. Furthermore, the antipsychotic drugs have high affinity for D2 dopamine receptor. We carried out the cloning of the genomic DNA for human D2 dopamine receptor and clarified the structure of this gene. Our isolated gene spans about 15 kbp and consists of seven exons interrupted by six introns. However, putative first exon was not yet identified. Spot blot hybridization analysis of cell sorter fractionated human chromosomal DNA with D2 receptor genomic DNA revealed the localization of this gene in the chromosome 11 fraction. We analyzed human genomic DNA by Southern blot hybridization with D2 dopamine receptor genomic DNA as a probe, but so far we could not find RFLP. Northern blot analyses of brain RNA of several animals and rat brain RNA after various treatments were carried out. Developmental changes of D2 dopamine receptor mRNA were observed in the rat brains.
Department of Neuropharmacology, Niigata University, Japan.
K
K
Araki
R
R
Kuwano
K
K
Morii
S
S
Hayashi
S
S
Minoshima
N
N
Shimizu
T
T
Katagiri
H
H
Usui
T
T
Kumanishi
Y
Y
Takahashi
D000375:Aging / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001402:B-Lymphocytes; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D001921:Brain / Q000378:metabolism; D002460:Cell Line; D002874:Chromosome Mapping; D004247:DNA / Q000097:blood / Q000235:genetics / Q000302:isolation & purification; D005091:Exons; D015870:Gene Expression; D015698:Genomic Library; D006801:Humans; D007438:Introns; D007621:Karyotyping; D007962:Leukocytes / Q000378:metabolism; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D012150:Polymorphism, Restriction Fragment Length; D011487:Protein Conformation; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017448:Receptors, Dopamine D2 / Q000235:genetics*; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid
D009838:Oligodeoxyribonucleotides; D012333:RNA, Messenger; D017448:Receptors, Dopamine D2; D004247:DNA