Immunoelectron microscopic epitope locations of titin in rabbit heart muscle.
The location of cardiac titin epitopes in the sarcomere of rabbit cardiac, atrial and ventricular muscle was studied by using polyclonal antibodies against skeletal muscle titin. The results show that incubation with the antibody leads to the appearance of four electron-dense stripes in the A band of both atrial and ventricular cardiac muscle. The location and intensity of these stripes were identical to those observed in skeletal muscle. In conclusion we demonstrate that titins from skeletal and cardiac muscles share some common antigenic determinants.
CNR Unit for Muscle Biology and Physiopathology, University of Padova, Italy.
S
S
Pierobon-Bormioli
D
D
Biral
R
R
Betto
G
G
Salviati
D000818:Animals; D000906:Antibodies / Q000276:immunology; D015153:Blotting, Western; D064211:Connectin; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000032:analysis* / Q000276:immunology; D007150:Immunohistochemistry; D008297:Male; D016253:Microscopy, Immunoelectron; D009124:Muscle Proteins / Q000032:analysis* / Q000276:immunology; D009206:Myocardium / Q000737:chemistry* / Q000648:ultrastructure; D011494:Protein Kinases; D011817:Rabbits
D000906:Antibodies; D064211:Connectin; D000939:Epitopes; D009124:Muscle Proteins; D011494:Protein Kinases