Prediction of doxorubicin resistance in gastrointestinal cancer by P-glycoprotein staining.
Feasibility of immunohistochemical staining of P-glycoprotein for the prediction of doxorubicin resistance in gastrointestinal cancers was examined. Among 10 cancer cell lines which consist of two gastric cancer cell lines and eight colon cancer cell lines, seven cell lines were stained positively by the monoclonal antibody to P-glycoprotein, C219. In consequence of the evaluation on the effect of doxorubicin on these tumour cells by means of succinic dehydrogenase inhibition test (SDI test), zero out of seven cell lines stained positively by C219 was sensitive to doxorubicin, but two out of three cell lines stained negatively were sensitive. Among 23 fresh surgical specimens of gastrointestinal cancers which consisted of 15 gastric cancers and eight colon cancers, seven tumour tissues were stained positively by C219. All P-glycoprotein positive tumours were resistant to doxorubicin. On the other hand, four of 16 P-glycoprotein tumours were sensitive to doxorubicin. These data indicate that positively stained cancer cells by C219 are resistant to doxorubicin.
Department of Surgery II, Nagoya University School of Medicine, Japan.
M
M
Yamauchi
H
H
Kumazawa
T
T
Satta
I
I
Sugawara
K
K
Isobe
Y
Y
Kodera
K
K
Ito
T
T
Watanabe
H
H
Takagi
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D002460:Cell Line; D003110:Colonic Neoplasms / Q000473:pathology; D004317:Doxorubicin / Q000627:therapeutic use*; D004351:Drug Resistance; D005069:Evaluation Studies as Topic; D005770:Gastrointestinal Neoplasms / Q000737:chemistry / Q000188:drug therapy*; D006801:Humans; D007150:Immunohistochemistry; D008562:Membrane Glycoproteins / Q000032:analysis*; D013274:Stomach Neoplasms / Q000473:pathology
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D008562:Membrane Glycoproteins; D004317:Doxorubicin