Transplantation of human neuroblastoma cells, catecholaminergic and non-catecholaminergic: effects on rotational behavior in Parkinson's rat model.
Cultured human catecholaminergic and non-catecholaminergic donor cells were used in neural transplantation experiments in a rat model of Parkinson's disease. Using two different human catecholaminergic neuroblastoma cell lines, one control non-catecholaminergic neuroblastoma cell line, and one sham control (tissue culture medium), transplants were made into the striatum using a modified Ungerstedt hemiparkinsonian rat model. Significant decreases in apomorphine-induced rotational behavior were produced by two of three catecholaminergic cell lines. Grafted cells staining positively for tyrosine hydroxylase (TH) and catecholamine fluorescence indicated viable catecholamine activity in the two cell lines which produced reductions in rotational behavior. Catecholamine fluorescence was not detected in either of the two controls. These data suggest a link between catecholamine secretion by transplanted cells and motor improvement using a rat rotational behavior model.
Department of Neurology, University of California, Los Angeles School of Medicine 90024-1769.
J S
JS
Manaster
T
T
Feuerman
C P
CP
Reynolds
C H
CH
Markham
D000818:Animals; D001058:Apomorphine / Q000494:pharmacology; D001932:Brain Neoplasms / Q000201:enzymology / Q000503:physiopathology*; D016380:Brain Tissue Transplantation / Q000502:physiology*; D002395:Catecholamines / Q000502:physiology*; D006651:Histocytochemistry; D006801:Humans; D009368:Neoplasm Transplantation / Q000502:physiology*; D009447:Neuroblastoma / Q000201:enzymology / Q000503:physiopathology*; D016627:Oxidopamine; D010300:Parkinson Disease / Q000503:physiopathology*; D051381:Rats; D011919:Rats, Inbred Strains; D012399:Rotation; D013239:Stereotyped Behavior / Q000502:physiology*; D013378:Substantia Nigra / Q000502:physiology; D014184:Transplantation, Homologous; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
D002395:Catecholamines; D016627:Oxidopamine; D014446:Tyrosine 3-Monooxygenase; D001058:Apomorphine