Adherence of pathogenic and non-pathogenic Entamoeba histolytica strains to neutrophils.
The adherence of polymorphonuclear leukocytes (PMNs) to eight pathogenic and nine nonpathogenic strains of Entamoeba histolytica was examined. No difference between pathogenic and nonpathogenic strains was found. The addition of different carbohydrates confirmed the importance of the 170-kDa lectin of E. histolytica in binding to PMNs, corroborated by the finding that treatment of PMNs with galactosidase inhibited adherence. Inhibition of the microfilament system of E. histolytica using cytochalasin B resulted in a loss of adherence to PMNs. Inhibition of the microtubule system using nocodazole did not affect adherence. Preincubation of the trophozoites with serum resulted in enhanced adherence, but the serum factor responsible for this effect could not be identified. Fibronectin, vitronectin, integrins (CD11/CD18 molecules), complement, and mannose-binding protein did not seem to mediate adherence between E. histolytica and PMNs. In summary, these results indicate that defective adherence mechanisms are not a common feature of nonpathogenic E. histolytica strains.
Bernhard Nocht Institute for Tropical Medicine, Clinical Department, Hamburg, Federal Republic of Germany.
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Burchard
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Bilke
D000116:Acetylgalactosamine / Q000494:pharmacology; D000818:Animals; D001667:Binding, Competitive; D001798:Blood Proteins / Q000494:pharmacology; D002241:Carbohydrates / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects; D002478:Cells, Cultured; D003571:Cytochalasin B / Q000494:pharmacology; D003599:Cytoskeleton / Q000187:drug effects; D004748:Entamoeba histolytica / Q000187:drug effects / Q000378:metabolism* / Q000472:pathogenicity; D005353:Fibronectins / Q000494:pharmacology; D005696:Galactosidases / Q000494:pharmacology; D006023:Glycoproteins / Q000494:pharmacology; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D016023:Integrins / Q000378:metabolism; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism / Q000469:parasitology*; D012397:Rosette Formation; D019096:Vitronectin
D001798:Blood Proteins; D002241:Carbohydrates; D005353:Fibronectins; D006023:Glycoproteins; D007106:Immune Sera; D016023:Integrins; D019096:Vitronectin; D003571:Cytochalasin B; D005696:Galactosidases; D000116:Acetylgalactosamine