Large-scale insect cell culture: methods, applications and products.
The primary development in large-scale insect cell culture over the past year has been the continuing accumulation of documented evidence (fundamental and applied) that conventional aerated stirred-tank and air-lift bioreactors may be employed for insect cell cultivation and recombinant protein production, provided that air sparging, agitation, and the addition to the medium of Pluronic F-68 and methyl cellulose polymers are carefully controlled.
Goosen
M F
MF
Queen's University, Ontario, Canada.
D000818:Animals; D001709:Biotechnology / Q000379:methods; D002478:Cells, Cultured; D007313:Insecta / Q000166:cytology*