Carbachol-induced theta-like activity in entorhinal cortex slices.
The present study was conducted for two purposes: the first was to evaluate whether activation of cholinergic receptors of the entorhinal cortex in vitro (complete deafferentation) with carbachol (100 microM) was capable of producing theta (theta)-like slow activity. The second purpose was to determine whether carbachol-induced slow waveforms were mediated by muscarinic or nicotinic receptors. We demonstrated that carbachol was capable of producing theta-like slow activity. This activity was not altered by nicotinic antagonists, (+)-tubocurarine and hexametonium. Atropine and scopolamine, in contrast, completely blocked in vitro induced slow waves, indicating entorhinal muscarinic receptors to be actively involved in the mechanism generating cholinergic theta rhythm.
Laboratory of Limbic System Physiology, University of Lódź, Poland.
J
J
Konopacki
H
H
Gołebiewski
B
B
Eckersdorf
D000818:Animals; D001285:Atropine / Q000494:pharmacology; D002217:Carbachol / Q000494:pharmacology*; D018738:Hexamethonium; D006584:Hexamethonium Compounds / Q000494:pharmacology; D006624:Hippocampus / Q000187:drug effects*; D066298:In Vitro Techniques; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D011950:Receptors, Cholinergic / Q000187:drug effects*; D011976:Receptors, Muscarinic / Q000187:drug effects / Q000502:physiology*; D011978:Receptors, Nicotinic / Q000187:drug effects / Q000502:physiology*; D012601:Scopolamine / Q000494:pharmacology; D013826:Theta Rhythm / Q000187:drug effects*; D014403:Tubocurarine / Q000494:pharmacology
D006584:Hexamethonium Compounds; D011950:Receptors, Cholinergic; D011976:Receptors, Muscarinic; D011978:Receptors, Nicotinic; D018738:Hexamethonium; D001285:Atropine; D002217:Carbachol; D012601:Scopolamine; D014403:Tubocurarine