Tissue- and developmental-stage-specific methylation in the two kidney promoters of the rat gamma-glutamyl transpeptidase gene.
We have investigated, using DNA methylation patterning, the site-specific methylation of promoters I and II of the rat gamma-glutamyl transpeptidase gene. This analysis was done in fetal, newborn and adult rat kidney, in which promoters I and II are progressively active during development, as well as in rat liver, which never expresses mRNAs from these two promoters. During kidney development, a progressive demethylation occurs in the promoter I and II region, specially at the level of the most proximal MspI site of promoter II. A progressive reorganization of the methylated sites within the 5' end of the gene also occurs during liver development.
Unité 99 INSERM, Hôpital Henri Monodor, Créteil, France.
J H
JH
Baik
S
S
Siegrist
G
G
Giuili
O
O
Lahuna
F
F
Bulle
G
G
Guellaën
D000818:Animals; D015139:Blotting, Southern; D004247:DNA / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D007668:Kidney / Q000201:enzymology* / Q000254:growth & development; D008745:Methylation; D009928:Organ Specificity / Q000235:genetics; D011401:Promoter Regions, Genetic; D051381:Rats; D005723:gamma-Glutamyltransferase / Q000235:genetics* / Q000378:metabolism
D004247:DNA; D005723:gamma-Glutamyltransferase