Cytosolic long-chain acyl-CoA hydrolase, a suitable parameter to measure hepatic response to peroxisome proliferators.
The possibility of using cytosolic long-chain acyl-CoA as a parameter to measure the response of liver to peroxisome proliferators was studied. A subcutaneous (s.c.) injection of perfluorooctanoic acid (PFOA) to male Wistar rats caused an increase in activity of cytosolic long-chain acyl-CoA hydrolase. This increase in activity seems to be due to enzyme induction, since it was prevented by simultaneous administration of cycloheximide or actinomycin D with PFOA. The activity of cytosolic long-chain acyl-CoA hydrolase was increased in a dose-dependent manner by the administration of three peroxisome proliferators with diverse chemical structures: alpha-(p-chlorophenoxy)isobutyric acid (clofibric acid), 2,2'-(decamethylenedithio)diethanol (tiadenol) and PFOA. The increased activity produced by clofibric acid lasted throughout a 22-week treatment. A good correlation was found between the activities of cytosolic long-chain acyl-CoA hydrolase and peroxisomal beta-oxidation induced by the administration of the peroxisome proliferators. These results indicate that cytosolic long-chain acyl-CoA hydrolase is a suitable parameter for measuring the response of rat liver to challenges by peroxisome proliferators.
Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University, Japan.
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Kawashima
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Kozuka
D000284:Administration, Oral; D000818:Animals; D002210:Caprylates / Q000494:pharmacology*; D002995:Clofibric Acid / Q000494:pharmacology; D004347:Drug Interactions; D004790:Enzyme Induction / Q000187:drug effects; D005233:Fatty Alcohols / Q000494:pharmacology; D005466:Fluorocarbons / Q000494:pharmacology*; D000960:Hypolipidemic Agents / Q000494:pharmacology; D008099:Liver / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D010170:Palmitoyl-CoA Hydrolase / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D002210:Caprylates; D005233:Fatty Alcohols; D005466:Fluorocarbons; D000960:Hypolipidemic Agents; C004568:tiadenol; D002995:Clofibric Acid; C023036:perfluorooctanoic acid; D010170:Palmitoyl-CoA Hydrolase