Acetyl-coenzyme A carboxylase mRNA metabolism in the rat liver.
The acetyl-coenzyme A carboxylase (ACC) gene contains two promoters (PI and PII), both of which are active in the liver. Various physiological stimuli affect one, or both of the promoters of the ACC gene, and result in the generation of two classes of ACC mRNAs which differ in the composition of their 5' untranslated regions (5' UTR). We have analyzed the amounts of the two major mRNAs species that are generated from each of these promoters in order to examine the regulation of ACC gene activity in the liver under different physiological conditions. Our findings can be summarized as follows: (1) In liver from normal animals, fed a complete laboratory chow ad libitum, the level of class 2 ACC mRNA species generated by PII is very low. These mRNA species disappear on starvation. Refeeding starved animals with a fat-free diet stimulates both PI and PII with different time courses of induction: PII responds quickly and PII gene products accumulate to maximum levels within 18 hours, while the PI response, as measured by the accumulation of class 1 mRNAs, shows a lag period of 6 hours before reaching maximal levels at the end of a 24-hour refeeding period. The half-lives estimated from the induction kinetics were 4.4 hours for class 2 mRNAs and 11.8 hours for class 1 mRNAs. Reinstatement of starvation causes an almost instantaneous disappearance of class 1 mRNA species, as compared with class 2 mRNA species. This rapid decay of PI transcripts suggests that factors stabilizing this class of ACC mRNAs contribute to the steady-state levels reached after the dietary induction.(ABSTRACT TRUNCATED AT 250 WORDS)
Biochemistry Department, West Lafayette, IN 47907.
F
F
López-Casillas
M V
MV
Ponce-Castañeda
K H
KH
Kim
D000103:Acetyl-CoA Carboxylase / Q000235:genetics*; D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy / Q000201:enzymology* / Q000235:genetics; D004435:Eating; D015870:Gene Expression / Q000187:drug effects; D007328:Insulin / Q000627:therapeutic use; D008099:Liver / Q000201:enzymology*; D008297:Male; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013217:Starvation; D013997:Time Factors
D007328:Insulin; D012333:RNA, Messenger; D012313:RNA; D000103:Acetyl-CoA Carboxylase