A novel Vero cell line for use as a mammalian host-vector system in serum-free medium.
We have established a novel cell line from a Vero cell derivative that is useful for expression of exogenous genes and protein production. Parental Vero-317 cells can grow in biotin-containing Eagle's MEM without supplements. By transforming this cell line with replication origin-defective SV40 DNA, which contains a temperature-sensitive tsA58 large T antigen gene, we established the Verots S3 cell line that amplified a SV40-origin containing plasmid. The cell line expressed a human growth hormone (hGH) gene insert with higher efficiency than COS-7 cells in 5% serum-containing MEM and could grow and continue hGH expression in protein-free MEM. However, temperature-sensitive shut down of hGH production was observed not immediately but 3 days after the temperature shift from 33 degrees C to 39.5 degrees C.
RIKEN Cell Bank, RIKEN Institute of Physical and Chemical Research, Tsukuba Science City, Japan.
T
T
Ohno
X
X
Wang
J
J
Kurashima
K
K
Saijo-Kurita
M
M
Hirono
D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000096:biosynthesis; D015139:Blotting, Southern; D002455:Cell Division; D002460:Cell Line; D016895:Culture Media, Serum-Free; D015870:Gene Expression; D005822:Genetic Vectors; D013006:Growth Hormone / Q000235:genetics / Q000378:metabolism; D007700:Kinetics; D010957:Plasmids; D015183:Restriction Mapping; D013696:Temperature; D014162:Transfection; D014170:Transformation, Genetic; D014709:Vero Cells / Q000166:cytology / Q000378:metabolism*
D000952:Antigens, Polyomavirus Transforming; D016895:Culture Media, Serum-Free; D013006:Growth Hormone