Structure of a ricin mutant showing rescue of activity by a noncatalytic residue.
Ricin A chain is an N-glycosidase which removes a single adenine base from a conservative loop of 28S rRNA, thereby inactivating eukaryotic ribosomes. The mechanism of action has been proposed to include transition-state stabilization of an oxycarbonium ion on the substrate ribose by interaction with Glu 177. Conversion of Glu 177 to Gln reduces activity nearly 200-fold [Ready, M. P., Kim, Y., & Robertus, J. D. (1991) Proteins: Struct., Funct., Genet. 10, 270-278] while conversion to Ala (E177A) reduces activity only 20-fold [Schlossman, D., Withers, D., Welsh, P., Alexander, A., Robertus, J., & Frankel, A. (1989) Mol. Cell. Biol. 9, 5012-5021]. X-ray analysis of the latter mutant protein shows that a residue at the edge of the active site, Glu 208, rotates into the space left vacant by the mutation. Its rearranged carboxylate partially substitutes for that of Glu 177. This is equivalent to the rescue of enzyme activity by a second-site reversion. Kinetic analysis shows the E177A mutation affects kcat and not Km, consistent with the notion that the carboxylate serves in transition-state stabilization.
Department of Chemistry and Biochemistry, Clayton Foundation Biochemical Institute, University of Texas, Austin 78712.
Y
Y
Kim
D
D
Mlsna
A F
AF
Monzingo
M P
MP
Ready
A
A
Frankel
J D
JD
Robertus
D000595:Amino Acid Sequence; D000818:Animals; D001156:Artemia / Q000737:chemistry; D001665:Binding Sites; D002384:Catalysis; D004926:Escherichia coli / Q000737:chemistry; D005971:Glutamates / Q000737:chemistry; D018698:Glutamic Acid; D007700:Kinetics; D009154:Mutation; D012276:Ricin / Q000737:chemistry* / Q000235:genetics; D013329:Structure-Activity Relationship
D005971:Glutamates; D018698:Glutamic Acid; D012276:Ricin