Modelling the growth and protein production by insect cells following infection by a recombinant baculovirus in suspension culture.
A mathematical model has been developed to describe the growth and infection of insect cells by recombinant baculoviruses. The model parameters were determined from a series of independent experiments involving batch suspension culture. The profiles generated by the model for cell growth, virus production and protein production agree with those observed in experiments. Presently, the model simulates only systems where cells are not growth-limited. The model is useful in aiding the design and optimization of large-scale systems for production of biological insecticides as well as recombinant proteins and in delineating those areas which are limiting the process and require further, more fundamental, investigation.
Power
J
J
Department of Chemical Engineering, University of Queensland, Brisbane, Australia.
Greenfield
P F
PF
Nielsen
L
L
Reid
S
S
D000818:Animals; D016367:Baculoviridae / Q000502:physiology*; D002455:Cell Division / Q000502:physiology; D002478:Cells, Cultured; D003198:Computer Simulation; D007313:Insecta / Q000166:cytology / Q000378:metabolism*; D008954:Models, Biological; D011994:Recombinant Proteins / Q000096:biosynthesis
D011994:Recombinant Proteins