Different DNA changes in primary and recurrent hepatocellular carcinoma.
DNA restriction fragment length polymorphism analysis was carried out on a primary and recurrent hepatocellular carcinoma in a hepatitis B virus negative patient. For the primary tumour, allele losses were found on the short arm of chromosome 17 (probe: p144-D6, 17p13) and the long arm of chromosome 5 with the probe Lambda MS8 (5q35-qter); other probes showed either no allele loss or a non-informative pattern. The recurrent cancer also showed allele loss with p144-D6, but not with Lambda MS8. In addition, the recurrent tumour had allele losses with Lambda MS43 (12q24.3-qter), pYNZ22 (17p13), and DNA rearrangement revealed by the probe Lambda MS32 (1q42-43), a pattern not seen in the primary lesion. These results indicate that the second hepatocellular carcinoma was of independent clonality and probably represents a de novo neoplasm rather than a recurrence.
University Department of Surgery, Royal Free Hospital School of Medicine, London.
S F
SF
Ding
R P
RP
Jalleh
C B
CB
Wood
L
L
Bowles
J D
JD
Delhanty
J
J
Dooley
N A
NA
Habib
D000368:Aged; D001345:Autoradiography; D006528:Carcinoma, Hepatocellular / Q000235:genetics*; D002872:Chromosome Deletion; D015342:DNA Probes; D004273:DNA, Neoplasm / Q000235:genetics*; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics*; D008297:Male; D009364:Neoplasm Recurrence, Local / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length
D015342:DNA Probes; D004273:DNA, Neoplasm