Thermolytical techniques to characterize fungal polysaccharides and bacterial lipopolysaccharides.
The present work constitutes an entirely novel contribution in the scope of microbiology and especially in taxonomy, introducing thermolysis curves as a rapid method of characterization of fungal polysaccharides and bacterial lipopolysaccharides. The thermal analysis techniques applied were thermogravimetry and derivative thermogravimetry (TG-DTG), differential thermal analysis (DTA), and differential scanning calorimetry (DSC). Each thermogram of a sample is represented by one or a few temperatures and, in DSC, by complementary enthalpy data. The temperatures of the thermograms from structurally unknown polysaccharides are compared with those used as references, and thus, information on their composition, linkage types, and anomeric configuration can be deduced. The situation is more complicated for bacterial lipopolysaccharides, but in whatever mode, a structural estimation is always possible. In the course of the development and validation of the thermal method, structural findings on relative stabilities of linkage types (valuable in carbohydrate research) have been recognized and are therefore also described in this work.
Departamento de Microbiología, Facultad de Medicina, Valladolid, Spain.
M C
MC
Ramos-Sánchez
A
A
Rodríguez-Torres
J A
JA
Leal
F J
FJ
Martín-Gil
J
J
Martín-Gil
D001709:Biotechnology; D002152:Calorimetry, Differential Scanning; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D004055:Differential Thermal Analysis; D005658:Fungi / Q000737:chemistry / Q000145:classification; D006090:Gram-Negative Bacteria / Q000737:chemistry / Q000145:classification; D008070:Lipopolysaccharides / Q000737:chemistry*; D008969:Molecular Sequence Data; D011134:Polysaccharides / Q000737:chemistry*; D013818:Thermogravimetry
D008070:Lipopolysaccharides; D011134:Polysaccharides