Biotransformation of digitoxigenin by ginseng hairy root cultures.
Five new compounds (three esters and two glycosides) and seven previously reported compounds were isolated as biotransformation products of digitoxigenin by ginseng hairy root cultures. The new esters and glycosides were elucidated as digitoxigenin stearate, digitoxigenin palmitate, digitoxigenin myristate, 3-epidigitoxigenin beta-D-gentiobioside and digitoxigenin beta-D-sophoroside using 1H and 13CNMR and FAB mass spectral data. Biotransformations involving esterification (of stearic acid, palmitic acid, myristic acid and lauric acid) and glycosylation (of gentiobiose and sophorose) of digitoxigenin have been demonstrated for the first time in the plant cell and tissue cultures. The hairy roots showed high glycosylation ability to the digitoxigenin molecule.
Medicinal Plant Garden, School of Pharmaceutical Sciences, Kitasato University, Kanagawa, Japan.
K
K
Kawaguchi
M
M
Hirotani
T
T
Yoshikawa
T
T
Furuya
D000808:Angiotensinogen / Q000378:metabolism*; D001711:Biotransformation; D002478:Cells, Cultured; D004952:Esters; D006027:Glycosides / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D005894:Panax / Q000378:metabolism*; D010946:Plants, Medicinal
D004952:Esters; D006027:Glycosides; D000808:Angiotensinogen