[Soluble metabolic antigens of Trichinella spiralis: their isolation and characteristics].
Cultivation of Trichinella muscular larvae, purified by centrifugation in 20 ... 50% saccharose density gradient, in protein--free nutrient media at a dosage of 3.5-.10(3) lar./ml in the presence of insulin has made it possible to obtain a soluble antigen of Trichinella. It has been shown by means of electrophoresis in polyacrylamide gel that the soluble (secretory-excretory) antigen has three protein fractions while the somatic Trichinella antigen has 18 fractions. It has been shown that the soluble (secretory-excretory) antigen can be used for immobilization of erythrocytes on the surface that enables the sensitivity and specificity of serological methods for diagnosis of trichinellosis to be increased.
Kostetskiĭ
A F
AF
Vaserin
Iu I
IuI
D000818:Animals; D000947:Antigens, Helminth / Q000032:analysis / Q000302:isolation & purification* / Q000378:metabolism; D003470:Culture Media; D004591:Electrophoresis, Polyacrylamide Gel; D006386:Hemagglutination Tests; D007814:Larva / Q000276:immunology / Q000378:metabolism; D009132:Muscles / Q000469:parasitology; D012995:Solubility; D013997:Time Factors; D017160:Trichinella spiralis / Q000276:immunology* / Q000302:isolation & purification / Q000378:metabolism
D000947:Antigens, Helminth; D003470:Culture Media
Rastvorimye metabolicheskie antigeny Trichinella spiralis: poluchenie i kharakteristika.