[The study and evaluation of a method for identifying Escherichia coli by using fluorescent disks].
A study was carried out with 101 strains, 79 of Escherichia coli and 22 of other genera isolated from clinical samples at several hospitals in Havana form October 1989 to January 1990. In all the strains, beta-glycuronidase enzyme was detected in conditions established by our laboratory and was compared with the results reached by the ROCHE enterotube method. Of the 79 Escherichia coli strains, 74 were positive to the beta-glycuronidase detection test. Sensitivity was 94% and specificity, was 100%.
Departamiento de Diagnóstico Bioquímico y Microbiólogico, Centro Nacional de Investigaciones Científicas.
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Niebla Pérez
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Pascual Marqui
D001431:Bacteriological Techniques; D004926:Escherichia coli / Q000201:enzymology / Q000302:isolation & purification*; D005069:Evaluation Studies as Topic; D005453:Fluorescence; D005966:Glucuronidase / Q000032:analysis; D006801:Humans; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods; D014466:Ultraviolet Rays
D005966:Glucuronidase
Estudio y evaluación de un método de identificación de Escherichia coli mediante el empleo de discos fluorescentes.