Comparison of the amino acid sequences of the lectins from seeds of Dioclea lehmanni and Canavalia maritima.
The amino acid sequences of the major lectins from the seeds of Dioclea lehmanni and Canavalia maritima were determined by DABITC/PITC microsequence analysis of peptides derived from the proteins by enzymatic digestions with trypsin, chymotrypsin and the protease from S. aureus V8. These sequences were found to be very similar to those of the lectins from Dioclea grandiflora and Canavalia ensiformis (Con A). The D. lehmanni lectin was unusual amongst legume lectins in that it contained a single Cys.
Perez
G
G
Department of Chemistry, Universidad Nacional Bogota, Colombia.
Perez
C
C
Sousa-Cavada
B
B
Moreira
R
R
Richardson
M
M
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D007887:Fabaceae / Q000032:analysis*; D037102:Lectins / Q000737:chemistry* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D037121:Plant Lectins; D010940:Plant Proteins / Q000737:chemistry* / Q000302:isolation & purification; D010946:Plants, Medicinal; D012639:Seeds / Q000032:analysis; D012689:Sequence Homology, Nucleic Acid
D000596:Amino Acids; D037102:Lectins; D037121:Plant Lectins; D010940:Plant Proteins