A highly sensitive enzyme-linked immunosorbent assay for Clostridium perfringens enterotoxin.
A highly sensitive enzyme-linked immunosorbent assay (ELISA) for quantitation of Clostridium perfringens enterotoxin (CPE) by a sandwich method with polystyrene beads was elaborated. The ELISA was very sensitive with a detection limit of 1 pg/ml of CPE. Clostridium perfringens culture fluid did not interfere with the assay. This ELISA may be useful for the mass screening for Cl. perfringens producing small amounts of CPE.
Department of Veterinary Medicine, College of Agriculture, University of Osaka Prefecture, Japan.
T
T
Uemura
S
S
Yoshitake
D
D
Hu
T
T
Kajikawa
D001709:Biotechnology; D003015:Clostridium Infections / Q000209:etiology / Q000382:microbiology; D003016:Clostridium perfringens / Q000472:pathogenicity; D004768:Enterotoxins / Q000032:analysis* / Q000592:standards; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods* / Q000592:standards / Q000706:statistics & numerical data; D005517:Foodborne Diseases / Q000209:etiology / Q000382:microbiology; D006801:Humans; D012015:Reference Standards; D012680:Sensitivity and Specificity
D004768:Enterotoxins; C035598:enterotoxin, Clostridium