[In vitro culture of fibroblasts from pubic skin of women in diagnosis of androgen hypersensitivity].
Intracellular metabolism of testosterone was studied in in vitro cultured skin fibroblasts obtained from women with idiopathic hirsutism. A significantly higher rate of conversion of testosterone into 5-alpha-dihydrotestosterone was found in these women (4.78 +/- 2.08 fmol/microgram DNA/hour) as compared to that obtained for skin cultures of women without symptoms of androgenization (0.98 +/- 0.37 fmol/microgram DNA/hour). These results demonstrate that fibroblast culture may be useful in diagnosing the causes of hyperandrogenization in women with normal androgen secretion.
Zakład Endokrynologicznej Ginekologicznej AM w Białymstoku.
S
S
Wołczyński
W
W
Kuczyński
M
M
Kulikowski
M
M
Szamatowicz
D000328:Adult; D000728:Androgens / Q000378:metabolism; D002478:Cells, Cultured; D005260:Female; D005347:Fibroblasts / Q000378:metabolism*; D006628:Hirsutism / Q000209:etiology / Q000378:metabolism*; D006801:Humans; D012867:Skin / Q000378:metabolism*; D013739:Testosterone / Q000378:metabolism*
D000728:Androgens; D013739:Testosterone
Hodowla in vitro fibroblastów ze skóry łonowej kobiet w diagnostyce stanów nadwrazliwości na androgeny.