Expression of the signal transducing regions of CD4-like and lck genes in murine egg.
We previously demonstrated that the MHC class II molecule on murine sperm and CD4-like molecule on the egg vitelline membrane are involved in fertilization. In this study, the RNA transcripts in murine eggs corresponding to parts of the CD4 gene and lck gene in thymocytes were demonstrated by means of the reverse transcriptase (RT)/polymerase chain reaction (PCR) followed by the sequencing of the PCR products. The deduced sequences potentially encode the amino acid sequence of the transmembrane to the cytoplasmic domain of CD4 and that of the N-terminal domain of p56lck respectively. These findings indicate that a signal transducing complex similar to that in immune T cells is expressed at the transcriptional level in murine eggs.
Department of Immunology, University of Tokyo, Japan.
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Mori
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Gou
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Yoshida
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Saito
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Mori
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000276:immunology / Q000502:physiology; D015704:CD4 Antigens / Q000235:genetics* / Q000502:physiology; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D019860:Lymphocyte Specific Protein Tyrosine Kinase p56(lck); D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D009856:Oncogene Proteins, Viral / Q000235:genetics; D010063:Ovum / Q000276:immunology / Q000502:physiology*; D016133:Polymerase Chain Reaction / Q000379:methods; D011505:Protein-Tyrosine Kinases / Q000235:genetics*; D012194:RNA-Directed DNA Polymerase; D015183:Restriction Mapping; D015398:Signal Transduction; D013094:Spermatozoa / Q000276:immunology / Q000502:physiology; D013950:Thymus Gland / Q000276:immunology / Q000502:physiology; D014158:Transcription, Genetic
D015704:CD4 Antigens; D009838:Oligodeoxyribonucleotides; D009856:Oncogene Proteins, Viral; D004247:DNA; D011505:Protein-Tyrosine Kinases; D019860:Lymphocyte Specific Protein Tyrosine Kinase p56(lck); D012194:RNA-Directed DNA Polymerase