Reductase activity encoded by the HM1 disease resistance gene in maize.
The HM1 gene in maize controls both race-specific resistance to the fungus Cochliobolus carbonum race 1 and expression of the NADPH (reduced form of nicotinamide adenine dinucleotide phosphate)-dependent HC toxin reductase (HCTR), which inactivates HC toxin, a cyclic tetrapeptide produced by the fungus to permit infection. Several HM1 alleles were generated and cloned by transposon-induced mutagenesis. The sequence of wild-type HM1 shares homology with dihydroflavonol-4-reductase genes from maize, petunia, and snap-dragon. Sequence homology is greatest in the beta alpha beta-dinucleotide binding fold that is conserved among NADPH- and NADH (reduced form of nicotinamide adenine dinucleotide)-dependent reductases and dehydrogenases. This indicates that HM1 encodes HCTR.
Johal
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GS
Department of Biotechnology Research, Pioneer Hi-Bred International, Inc., Johnston, IA 50131.
Briggs
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SP
D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004247:DNA / Q000737:chemistry / Q000235:genetics; D017343:Genes, Plant; D006375:Helminthosporium; D007438:Introns; D008969:Molecular Sequence Data; D009249:NADP / Q000494:pharmacology; D009693:Nucleic Acid Hybridization; D010088:Oxidoreductases / Q000737:chemistry / Q000235:genetics*; D010456:Peptides, Cyclic / Q000037:antagonists & inhibitors; D010935:Plant Diseases; D010940:Plant Proteins; D012150:Polymorphism, Restriction Fragment Length; D012326:RNA Splicing; D012333:RNA, Messenger / Q000235:genetics; D003313:Zea mays / Q000201:enzymology / Q000235:genetics*
D010456:Peptides, Cyclic; D010940:Plant Proteins; D012333:RNA, Messenger; D009249:NADP; C037654:HC toxin; D004247:DNA; C078135:HC toxin reductase, Zea mays; D010088:Oxidoreductases