Growth of poliovirus using alginate entrapped-anchorage dependent Vero cells.
Obligatory anchorage dependant Vero cells were successfully grown in gelatinous-like macrocarriers made of calcium alginate. Entrapped single cells were immobilized within the polymerized alginate matrix divide to form large spherical clumps of cells. A cell density of 17 x 10(6) cells/ml of alginate with over 95% viability was obtained after 14 days in spinner flasks. When subjected to poliovirus type I infection, spherical masses of Vero cells progressively showed extensive cytopathic effect but remained entrapped in the alginate matrix of the macrocarriers. Virus was released into a cellular debris free-like supernatant and reached a peak titer of 10(8.0) TCID50/ml after 72 hours.
Division of Viral Vaccines, Institut Armand-Frappier, Québec, Canada.
P
P
Trépanier
R
R
Dugré
R
R
Alain
J
J
Lapierre
D000464:Alginates; D000818:Animals; D002448:Cell Adhesion; D002452:Cell Count; D003584:Cytological Techniques; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D017955:Poliovirus / Q000254:growth & development*; D014709:Vero Cells; D014776:Virus Cultivation / Q000379:methods*
D000464:Alginates; D006603:Hexuronic Acids; D020723:Glucuronic Acid