Protein recovery from reversed micellar solutions through contact with a pressurized gas phase.
We describe a new process for the recovery of encapsulated protein from reversed micellar solution in concentrated form. The method involves desolubilization of the protein by decreasing solvent density through gas dissolution. Under appropriate thermodynamic conditions, the micellar water pool can be converted to clathrate hydrates. Protein recovery is facilitated by clathrate hydrate formation, which causes the desolubilized protein to exist in a solid phase, distinct from the micellar supernatant. The process is carried out without any ionic strength or pH modification.
Chemical Engineering Department, Tulane University, New Orleans, Louisiana 70118.
J B
JB
Phillips
H
H
Nguyen
V T
VT
John
D004143:Dioctyl Sulfosuccinic Acid; D005030:Ethylenes; D005740:Gases; D008823:Micelles; D011312:Pressure; D011506:Proteins / Q000302:isolation & purification*; D012995:Solubility; D012996:Solutions; D014867:Water
D005030:Ethylenes; D005740:Gases; D008823:Micelles; D011506:Proteins; D012996:Solutions; D014867:Water; D004143:Dioctyl Sulfosuccinic Acid; C036216:ethylene