Comparison of a competitive enzyme immunoassay kit and the infant mouse assay for detecting Escherichia coli heat-stable enterotoxin.
A commercial competitive enzyme immunoassay kit, Escherichia coli ST EIA, was compared with the conventional infant mouse assay for sensitivity and specificity in detecting E. coli heat-stable enterotoxin. Thirty-one of 46 strains of E. coli tested were positive by both assays, while 15 strains were negative. The sensitivity of the ST EIA kit was up to 64-fold lower than the infant mouse assay.
Bureau of Microbial Hazards, Health & Welfare Canada, Ottawa, Ontario.
S
S
Stavric
B
B
Buchanan
J
J
Speirs
D000818:Animals; D000831:Animals, Newborn; D001427:Bacterial Toxins / Q000032:analysis*; D001667:Binding, Competitive; D001681:Biological Assay; D004768:Enterotoxins / Q000032:analysis*; D004926:Escherichia coli; D029968:Escherichia coli Proteins; D007124:Immunoenzyme Techniques; D051379:Mice; D011237:Predictive Value of Tests; D011933:Reagent Kits, Diagnostic
D001427:Bacterial Toxins; D004768:Enterotoxins; D029968:Escherichia coli Proteins; D011933:Reagent Kits, Diagnostic; C034151:heat stable toxin (E coli)