Assignment of the chicken tyrosine hydroxylase gene to chromosome 6 by FISH.
The gene for tyrosine hydroxylase, the first and rate-limiting enzyme in the biosynthetic pathway of catecholamine neurotransmitters, has been localized in situ to chromosome 6 in the chicken. It is the first DNA marker to be reported for this telocentric macrochromosome. Use of a 45-kb biotinylated chicken-specific cosmid probe and a sensitive fluorescent detection system proved to be highly efficient, with over 90% of metaphases showing positive hybridization signals on one or (usually) both chromosome 6 homologs, in physically mapping this single-gene locus.
Department of Human Genetics, University of Würzburg, FRG.
M
M
Dominguez-Steglich
A
A
Carrier
C
C
Auffray
M
M
Schmid
D000818:Animals; D002645:Chickens / Q000235:genetics*; D002874:Chromosome Mapping; D003360:Cosmids / Q000235:genetics; D015342:DNA Probes / Q000235:genetics; D005453:Fluorescence; D009693:Nucleic Acid Hybridization; D014446:Tyrosine 3-Monooxygenase / Q000235:genetics*
D015342:DNA Probes; D014446:Tyrosine 3-Monooxygenase