Total solution synthesis of human epidermal growth factor (h-EGF) by the assembly of nine building blocks.
Human epidermal growth factor (h-EGF) composed of 53 amino acids bearing three intramolecular disulfide bridges was synthesized by the maximum protecting solution method. The synthetic h-EGF coincided with recombinant h-EGF by reverse-phase HPLC, and the sites of three intramolecular disulfide bridges were ascertained by a thermolytic digestion. The synthetic h-EGF possessed m/z 6215.7 in its FAB-MS as expected, and exhibited compatible mitogenic activity.
Institute of Applied Biochemistry, University of Tsukuba, Japan.
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Shin
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Kaburaki
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Watanabe
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Munekata
D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D004815:Epidermal Growth Factor / Q000138:chemical synthesis* / Q000494:pharmacology; D006801:Humans; D006868:Hydrolysis; D051379:Mice; D008807:Mice, Inbred BALB C; D008934:Mitogens / Q000494:pharmacology; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D010455:Peptides / Q000138:chemical synthesis*; D011994:Recombinant Proteins / Q000494:pharmacology; D013820:Thermolysin
D008934:Mitogens; D010455:Peptides; D011994:Recombinant Proteins; D004815:Epidermal Growth Factor; D013820:Thermolysin