Detection of prodynorphin end products in lizard, turtle, and alligator brain extracts.
Heterologous radioimmunoassays (RIAs) for the mammalian prodynorphin end products, alpha-neo-endorphin, dynorphin A(1-17), dynorphin A(1-8), and dynorphin B(1-13) were used to screen brain extracts obtained from representatives of the major surviving orders of reptiles: Chelonia (Pseudemys scripta), Squamata (Anolis carolinensis), and Crocodylia (Alligator mississippiensis). Methanol/acid extracts of whole brains obtained from each species were separately fractionated by gel filtration chromatography and reversed-phase HPLC. In all three species, an immunoreactive form of alpha-neo-endorphin was detected with the same retention time as synthetic mammalian alpha-neo-endorphin following reversed-phase HPLC analysis. In all three species, reversed-phase HPLC analysis revealed a novel form of dynorphin B(1-13)-related immunoreactivity. With the available immunological probes, dynorphin A products were only detected in the Anolis brain extracts. Both dynorphin A(1-17) and dynorphin A(1-8) were detected in this species.
University of Denver, Department of Biological Sciences, CO 80208.
A M
AM
Goldsmith
C A
CA
Sei
V
V
Lance
R M
RM
Dores
D000489:Alligators and Crocodiles / Q000502:physiology; D000818:Animals; D005075:Biological Evolution; D001923:Brain Chemistry; D002851:Chromatography, High Pressure Liquid; D004399:Dynorphins / Q000032:analysis*; D004723:Endorphins / Q000032:analysis*; D004745:Enkephalins / Q000378:metabolism*; D005260:Female; D008116:Lizards / Q000502:physiology; D008297:Male; D011498:Protein Precursors / Q000032:analysis* / Q000378:metabolism*; D011863:Radioimmunoassay; D012104:Reptiles / Q000502:physiology*; D014020:Tissue Extracts / Q000737:chemistry; D014426:Turtles / Q000502:physiology
D004723:Endorphins; D004745:Enkephalins; D011498:Protein Precursors; D014020:Tissue Extracts; C025681:alpha-neoendorphin; D004399:Dynorphins; C033660:preproenkephalin