[The determination of benzodiazepines in biological objects on the Milichrome microcolumn liquid chromatograph].
1,4-benzodiazepines and benzophenols, the products of their hydrolytic cleavage, were measured by high-pressure liquid chromatography in expert material (stomach, liver, intestine, kidney samples). Milichrome microcolumn liquid chromatograph, designs 1 and 2, was employed, with a chromatography column packed with Separon C18 (5 microns) sorbent. A mixture of (NH4)2HPO4 (0.05 M) and acetonitryl in volume ratio 65:35 was used as the mobile phase in native compound analysis and that in volume ratio 45:55 for benzophenols and medazepam separation. The external standard method was used for quantitative estimation.
Bodrina
D E
DE
Eremin
S K
SK
Izotov
B N
BN
Tiapkina
R I
RI
D014151:Anti-Anxiety Agents / Q000032:analysis* / Q000302:isolation & purification; D001569:Benzodiazepines; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods; D006801:Humans; D007422:Intestines / Q000737:chemistry; D007668:Kidney / Q000737:chemistry; D008099:Liver / Q000737:chemistry; D012015:Reference Standards; D013270:Stomach / Q000737:chemistry
D014151:Anti-Anxiety Agents; D001569:Benzodiazepines
Opredelenie benzodiazepinov v biologicheskikh ob''ektakh na mikrokolonochnom zhidkostnom khromatografe "Milikhrom".