Interleukin 10 is a potent growth and differentiation factor for activated human B lymphocytes.
Interleukin 10 (IL-10), originally identified as a TH2 helper T-cell product able to inhibit cytokine production by TH1 cells, is highly homologous to BCRF1 (viral IL-10), an open reading frame in the Epstein-Barr virus genome. Here, we show that human and viral IL-10 stimulate DNA replication of B lymphocytes activated either via their antigen receptor or via their CD40 antigen. IL-4 and IL-10 display additive effects and induce a strong increase in the number of viable cells. Moreover, IL-10 induces activated B cells to secrete large amounts of IgG, IgA, and IgM, and the combination of IL-10 and IL-4 results in the secretion of the four immunoglobulin isotypes. Thus, IL-10 may play an important role in the amplification of humoral responses.
Schering-Plough, Laboratory for Immunological Research, Dardilly, France.
F
F
Rousset
E
E
Garcia
T
T
Defrance
C
C
Péronne
N
N
Vezzio
D H
DH
Hsu
R
R
Kastelein
K W
KW
Moore
J
J
Banchereau
D000917:Antibody Formation; D015703:Antigens, CD / Q000502:physiology; D000944:Antigens, Differentiation, B-Lymphocyte / Q000502:physiology; D001402:B-Lymphocytes / Q000502:physiology*; D019013:CD40 Antigens; D002454:Cell Differentiation; D002455:Cell Division; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D006801:Humans; D066298:In Vitro Techniques; D016753:Interleukin-10 / Q000494:pharmacology*; D015847:Interleukin-4 / Q000494:pharmacology; D008213:Lymphocyte Activation; D011947:Receptors, Antigen, B-Cell / Q000502:physiology; D011994:Recombinant Proteins / Q000494:pharmacology; D014764:Viral Proteins / Q000494:pharmacology
D015703:Antigens, CD; D000944:Antigens, Differentiation, B-Lymphocyte; D019013:CD40 Antigens; D011947:Receptors, Antigen, B-Cell; D011994:Recombinant Proteins; D014764:Viral Proteins; D016753:Interleukin-10; D015847:Interleukin-4; D004247:DNA