DNA fingerprinting of the mosquito pathogen Bacillus sphaericus with M13 DNA as a probe.
Hypervariable nucleotide sequences were detected in Bacillus sphaericus by hybridization with radioactively labelled M13 DNA. Different serotypes could be distinguished by their hybridization profiles. The appearance of bands common for mosquito-pathogenic strains and their absence in an apathogenic strain opens the probability that M13 could hybridize to specific alleles, related to insect toxicity.
Institute of Microbiology, Bulgarian Academy of Sciences, Sofia.
A N
AN
Abadjieva
R T
RT
Grigorova
V I
VI
Miteva
D000483:Alleles; D000818:Animals; D001407:Bacillus / Q000145:classification / Q000235:genetics*; D015342:DNA Probes; D004269:DNA, Bacterial / Q000032:analysis*; D009693:Nucleic Acid Hybridization; D009710:Nucleotide Mapping; D012150:Polymorphism, Restriction Fragment Length; D015183:Restriction Mapping; D012703:Serotyping
D015342:DNA Probes; D004269:DNA, Bacterial