Production and characterization of human gamma interferon from Escherichia coli.
The production of human gamma interferon as intracellular inclusion bodies in Escherichia coli, which simplified the purification process, is described. An expression plasmid carrying lipoprotein and the tryptophane promoters in tandem was used. Preparation of highly pure interferon was achieved using high resolution chromatography after denaturation and renaturation steps. Structural characteristics of this protein were verified by mass spectrometric analysis. Additional control tests have shown the suitability of the final product for clinical purposes.
Perez
L
L
Center for Genetic Engineering and Biotechnology (CIGB), Havana, Cuba.
Vega
J
J
Chuay
C
C
Menendez
A
A
Ubieta
R
R
Montero
M
M
Padron
G
G
Silva
A
A
Santizo
C
C
Besada
V
V
D000595:Amino Acid Sequence; D004926:Escherichia coli / Q000235:genetics*; D005285:Fermentation; D015964:Gene Expression Regulation, Bacterial; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis / Q000235:genetics*; D008074:Lipoproteins / Q000235:genetics; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D010957:Plasmids; D011401:Promoter Regions, Genetic; D011489:Protein Denaturation; D011994:Recombinant Proteins; D012091:Repetitive Sequences, Nucleic Acid; D014364:Tryptophan / Q000235:genetics
D008074:Lipoproteins; D011994:Recombinant Proteins; D007371:Interferon-gamma; D014364:Tryptophan