Differentiation-related changes in quantitative binding of immunomagnetic beads.
Experiments have been carried out to demonstrate that cell viability and phenotypic characteristics are not affected by exposure to constant magnetic fields or growth in the presence of Dynabeads. This paper also demonstrates that the changes in the surface concentration of the antigens, SSEA-1 (stage-specific embryonic antigen-1) and histocompatibility antigen (MHC H2-Db) during differentiation as determined by flow cytometric analysis, are mirrored by changes in the numbers of bound immunomagnetic particles (Dynabeads) targeted with monoclonal antibodies to these cell surface antigens. These results clearly indicate that the numbers of beads bound to cells reflect the numbers of specific surface antigens present on the cells.
Department of Biology, University of Essex, Colchester, England, UK.
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Swann
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Dealtry
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Rickwood
D000818:Animals; D002454:Cell Differentiation; D002455:Cell Division; D002470:Cell Survival; D006183:H-2 Antigens / Q000032:analysis; D064532:Histocompatibility Antigen H-2D; D016130:Immunophenotyping; D007519:Isoantigens / Q000032:analysis; D016256:Lewis X Antigen / Q000032:analysis; D008280:Magnetics; D008285:Major Histocompatibility Complex; D051379:Mice; D008863:Microspheres; D014407:Tumor Cells, Cultured / Q000166:cytology* / Q000276:immunology
D006183:H-2 Antigens; D064532:Histocompatibility Antigen H-2D; D007519:Isoantigens; D016256:Lewis X Antigen