Beta-eliminative cleavage of the acidic polysaccharide of Fusarium sp. M7-1 by an enzyme preparation of Cellulomonas sp.
By digestion with an enzyme preparation derived from a culture filtrate of Cellulomonas sp., an unsaturated disaccharide was produced accompanied by the release of mannose and beta 1----2 mannobiose from the acidic oligomer mixture that was obtained from the acidic polysaccharide of Fusarium sp. M7-1 by acetolysis. The unsaturated disaccharide produced was isolated and identified as 4-deoxy-L-threo-hex-4-enopyranouronosyl alpha(1----2)-D-galactose. The same unsaturated sugar linked to the polysaccharide was also produced accompanied by the release of mannose and beta 1----2 mannobiose from the acidic polysaccharide by the enzyme digestion.
Department of Bioresource Science, Faculty of Agriculture, Kagawa University, Japan.
S
S
Iwahara
T
T
Jikibara
S
S
Miki
K
K
Takegawa
D000192:Actinomycetales / Q000201:enzymology*; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D005670:Fusarium; D006868:Hydrolysis; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000302:isolation & purification; D011134:Polysaccharides
D009844:Oligosaccharides; D011134:Polysaccharides