DNA sequence analysis and comparison of the variable heavy and light chain regions of two IgM, monoclonal, anti-myelin associated glycoprotein antibodies.
The complete variable heavy and light chain gene sequences of two monoclonal, IgM, anti-myelin associated glycoprotein (MAG) antibodies associated with peripheral neuropathy, are presented. Comparative analysis of the two VH regions has revealed that they are 88% homologous to one another and are both members of the VH3 gene family. They are also highly homologous to a gene which is frequently utilized in the fetal B-cell repertoire. The V kappa light chain gene of one of the antibodies is 99% homologous to a V kappa II gene and the V lambda light chain gene of the other antibody is only 72% homologous to other known V lambda genes. Further analysis of V genes utilized by anti-MAG antibodies should reveal the structural basis for their binding activity.
Department of Neurology, College of Physicians and Surgeons of Columbia University, New York, NY 10032.
L A
LA
Spatz
M
M
Williams
B
B
Brender
R
R
Desai
N
N
Latov
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000235:genetics* / Q000276:immunology; D001483:Base Sequence; D015152:Blotting, Northern; D004247:DNA / Q000235:genetics*; D015321:Gene Rearrangement; D006801:Humans; D007075:Immunoglobulin M / Q000235:genetics*; D008969:Molecular Sequence Data; D009185:Myelin Proteins / Q000276:immunology*; D009186:Myelin Sheath / Q000276:immunology*; D018992:Myelin-Associated Glycoprotein
D000911:Antibodies, Monoclonal; D007075:Immunoglobulin M; D009185:Myelin Proteins; D018992:Myelin-Associated Glycoprotein; D004247:DNA