Immunization against polyoma tumors with synthetic peptides derived from the sequences of middle- and large-T antigens.
We have used 9 synthetic peptides corresponding to sequences of polyoma virus small-T, middle-T and large-T antigens as immunogens in order to map antigenic epitopes that can induce polyoma-tumor-specific immunity in different mouse strains. We found that immunization of mice with synthetic peptides derived from amino acid (aa) sequences common to all 3 T-antigens (aa 1-19), or sequences common to only middle-T and small-T (aa 162-176), as well as synthetic peptides unique for middle-T (aa 269-282 and 371-381), or unique for large-T (aa 108-124, 316-333 and 436-449) can induce immunity against polyoma tumors. The synthetic peptides can be divided into 3 types with regard to immunogenicity; (i) peptides that immunize in more than one mouse strain and may represent immunodominant sites, (ii) peptides that may be immunogenic in only one strain, and thus strain-specific, and finally (iii) peptides that do not immunize in the strains tested so far.
Department of Virology, Microbiology Central Laboratory, Stockholm, Sweden.
G
G
Reinholdsson-Ljunggren
T
T
Ramqvist
L
L
Ahrlund-Richter
T
T
Dalianis
D000595:Amino Acid Sequence; D000818:Animals; D000912:Antibodies, Neoplasm / Q000096:biosynthesis*; D000952:Antigens, Polyomavirus Transforming / Q000737:chemistry / Q000276:immunology*; D000939:Epitopes; D051379:Mice; D008969:Molecular Sequence Data; D009374:Neoplasms, Experimental / Q000276:immunology*; D010455:Peptides / Q000737:chemistry / Q000276:immunology; D011120:Polyomavirus / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000737:chemistry; D014765:Viral Vaccines / Q000737:chemistry
D000912:Antibodies, Neoplasm; D000952:Antigens, Polyomavirus Transforming; D000939:Epitopes; D010455:Peptides; D014614:Vaccines, Synthetic; D014765:Viral Vaccines