Evaluation of quinolinic acid induced excitotoxic neurodegeneration in rat striatum by quantitative magnetic resonance imaging in vivo.
Excitotoxic neurodegeneration in the rat striatum was induced by direct injection of quinolinic acid. The degree of damage was evaluated in vivo 1 day later by quantitative magnetic resonance imaging (MRI) and 7 days later in the same animals by measuring the activities of the neuronal marker enzymes choline acetyltransferase and glutamic acid decarboxylase. Striatal damage assessed using the two approaches was highly correlated. Moreover the cerebroprotective efficacy of the N-methyl-D-aspartate receptor antagonist CGP 40116 was indistinguishable based on all analytical parameters. MRI, however, was more reproducible than the enzymatic methods and was faster and simpler for routine analyses of excitotoxic damage and cerebroprotection in vivo.
Biology Research Laboratory, Pharmaceutical Division, Ciba-Geigy Ltd., Basel, Switzerland.
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D
Sauer
P R
PR
Allegrini
K H
KH
Thedinga
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L
Massieu
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H
Amacker
G E
GE
Fagg
D015763:2-Amino-5-phosphonovalerate / Q000031:analogs & derivatives / Q000494:pharmacology; D000818:Animals; D001921:Brain / Q000201:enzymology; D001929:Brain Edema / Q000473:pathology; D002795:Choline O-Acetyltransferase / Q000378:metabolism; D003342:Corpus Striatum / Q000473:pathology*; D005968:Glutamate Decarboxylase / Q000032:analysis / Q000378:metabolism; D008279:Magnetic Resonance Imaging; D008297:Male; D009270:Naloxone / Q000494:pharmacology; D009410:Nerve Degeneration / Q000187:drug effects*; D017378:Quinolinic Acid / Q000633:toxicity*; D051381:Rats; D011919:Rats, Inbred Strains; D016194:Receptors, N-Methyl-D-Aspartate / Q000037:antagonists & inhibitors
D016194:Receptors, N-Methyl-D-Aspartate; C064539:2-amino-4-methyl-5-phosphono-3-pentenoic acid; D009270:Naloxone; D015763:2-Amino-5-phosphonovalerate; D002795:Choline O-Acetyltransferase; D005968:Glutamate Decarboxylase; D017378:Quinolinic Acid