Isolation of steroidal glycoalkaloids from Solanum incanum by two countercurrent chromatographic methods.
Using a bioassay for inhibition of plant growth and a combination of two countercurrent chromatographies: rotation locular countercurrent chromatography and droplet countercurrent chromatography, two biologically active glycosidal alkaloids, solasonine and solamargine were isolated from fresh ripe fruit of Solanum incanum. The combination of these chromatographic techniques has established an efficient isolation of polar phytochemicals of steroidal glycoalkaloids.
Division of Entomology and Parasitology, College of Natural Resources, University of California, Berkeley 94720.
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Fukuhara
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Kubo
D002240:Carbohydrate Sequence; D008969:Molecular Sequence Data; D015394:Molecular Structure; D010840:Phytosterols / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D010937:Plant Growth Regulators / Q000737:chemistry / Q000302:isolation & purification*; D010944:Plants / Q000032:analysis*; D012991:Solanaceous Alkaloids / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology
D010840:Phytosterols; D010937:Plant Growth Regulators; D012991:Solanaceous Alkaloids; C026609:beta-solamarine; C026608:alpha-solamargine