Occurrence of free O-phosphoserine and O-phosphothreonine in porcine liver.
The occurrence of free O-phosphoserine and O-phosphothreonine in porcine liver is demonstrated. These amino acids were separated from the tissue extracts by anion- and cation-exchange chromatography and thin-layer chromatography, and were identified by gas chromatography with flame photometric detection and gas chromatography-mass spectrometry. The contents of O-phosphoserine and O-phosphothreonine in the liver were estimated to be 377 +/- 13 ng/g and 115 +/- 2 ng/g, respectively.
Faculty of Pharmaceutical Sciences, Okayama University, Japan.
H
H
Kataoka
N
N
Sakiyama
M
M
Makita
D000818:Animals; D008401:Gas Chromatography-Mass Spectrometry; D008099:Liver / Q000737:chemistry / Q000378:metabolism*; D010768:Phosphoserine / Q000032:analysis / Q000378:metabolism*; D010769:Phosphothreonine / Q000032:analysis / Q000378:metabolism*; D013552:Swine
D010769:Phosphothreonine; D010768:Phosphoserine