Monocyte adherence to fibronectin: role of CD11/CD18 integrins and relationship to other monocyte functions.
Adherence of monocytes to extracellular matrix components is critical for their accumulation at sites of infection. To gain insight into the factors that regulate monocyte recruitment, we have studied monocyte adherence with regard to the regulatory effects of bacterial lipopolysaccharide (LPS) and the mechanisms involved; moreover, we have contrasted the phenotypes of adherent and nonadherent cells. Our results show that only a minor subpopulation of monocytes (20-25%) adhere spontaneously to fibronectin and that LPS stimulated a threefold increase in the proportion of adherent cells. Basal adherence and LPS-stimulated adherence of monocytes to fibronectin were substantially mediated by CD11/CD18 integrins. Further studies revealed that spontaneously adherent monocytes were 14-fold more actively phagocytic, released 1.6-fold more superoxide anion, and contained 20-fold more peroxidase activity than nonadherent cells, whereas LPS-adherent cells had an intermediate phenotype. These results indicate that LPS may enhance the accumulation of monocytes with an antimicrobial phenotype and thereby promote resolution of tissue infection.
Department of Medicine, University of Utah Health Sciences Center, Salt Lake City 84132.
C A
CA
Owen
E J
EJ
Campbell
R A
RA
Stockley
D015703:Antigens, CD / Q000502:physiology*; D018845:CD11 Antigens; D018821:CD18 Antigens; D002448:Cell Adhesion; D005353:Fibronectins / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D007249:Inflammation / Q000503:physiopathology; D008070:Lipopolysaccharides / Q000008:administration & dosage; D009000:Monocytes / Q000166:cytology / Q000502:physiology*; D009842:Oligopeptides / Q000378:metabolism; D009195:Peroxidase / Q000378:metabolism; D010587:Phagocytosis; D016028:Receptors, Leukocyte-Adhesion / Q000502:physiology*; D013481:Superoxides / Q000378:metabolism; D013997:Time Factors
D015703:Antigens, CD; D018845:CD11 Antigens; D018821:CD18 Antigens; D005353:Fibronectins; D008070:Lipopolysaccharides; D009842:Oligopeptides; D016028:Receptors, Leukocyte-Adhesion; D013481:Superoxides; C046617:arginyl-glycyl-aspartyl-serine; D009195:Peroxidase