Specificity and T cell receptor beta chain usage of a human collagen type II-reactive T cell clone derived from a healthy individual.
Collagen type II (CII) is a cartilage-specific matrix compound well known as an inducer of an experimental, T cell-dependent autoimmune arthritis, a disease which shows some similarities to human rheumatoid arthritis. Here we report on an HLA-DR7-restricted human CD4 T cell clone (TC9), which was isolated from a healthy donor and recognizes human CII. After screening CNBr fragments of CII and tryptic fragments derived thereof, the T cell epitope could be mapped to amino acid residues 271-285 of the triple helical region of CII that are located within CNBr fragment 11 [alpha 1 (II) CB11]. This epitope was confirmed by a synthetic peptide stimulatory for TC9. The T cell receptor beta chain of TC9 was cloned using the polymerase chain reaction; it comprises V beta 6.7 and contains besides J beta 2.3 and C beta 2 an as yet undescribed sequence for the D segment.
Clinical Research Unit for Rheumatology, Friedrich-Alexander-Universität, Erlangen-Nürnberg, FRG.
T
T
Yan
H
H
Burkhardt
T
T
Ritter
B
B
Bröker
K H
KH
Mann
W M
WM
Bertling
K
K
von der Mark
F
F
Emmrich
D000328:Adult; D000595:Amino Acid Sequence; D001483:Base Sequence; D002999:Clone Cells; D003094:Collagen / Q000276:immunology*; D000939:Epitopes / Q000032:analysis; D015809:HLA-DR7 Antigen / Q000032:analysis; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics*; D013601:T-Lymphocytes / Q000276:immunology*
D000939:Epitopes; D015809:HLA-DR7 Antigen; D016693:Receptors, Antigen, T-Cell, alpha-beta; D003094:Collagen