Distributions of neuropeptide Y, vasoactive intestinal peptide and somatostatin in populations of postganglionic neurons innervating the rat kidney, spleen and intestine.
Some peripheral peptidergic nerves selectively innervate different types of tissue in abdominal organs. Neuropeptide Y- and vasoactive intestinal peptide-immunoreactive nerve terminals have been identified in the kidney, spleen and intestine and these peptides may have important physiological actions. Somatostatin has been found in sympathetic ganglia, and nerve terminals containing this peptide have been identified in the intestine. We have used fluorescent retrograde tracers to identify renal, splenic and mesenteric postganglionic neurons in rat sympathetic ganglia and then used immunocytochemistry to determine the proportions of these three identified groups of neurons displaying immunoreactivity for neuropeptide Y, vasoactive intestinal peptide and somatostatin. Most renal, splenic and mesenteric neurons were immunoreactive for neuropeptide Y and less than 1% of cells innervating these organs were immunoreactive for vasoactive intestinal peptide. Somatostatin immunoreactivity was present only in a small percentage of mesenteric neurons and not in renal or splenic neurons. The present study demonstrates that (i) the rat kidney, spleen and intestine do not differ in the proportion of innervation by neuropeptide Y-immunoreactive neurons, (ii) the solar plexus, splanchnic ganglion and chain ganglia (T12 and T13) provide very little vasoactive intestinal peptide-immunoreactive inputs to these organs, and (iii) somatostatin-immunoreactive neurons innervate the intestine but not the kidney or spleen.
John P. Robarts Research Institute, London, Ontario, Canada.
V
V
Chevendra
L C
LC
Weaver
D000818:Animals; D005728:Ganglia, Sympathetic / Q000166:cytology / Q000502:physiology; D006168:Guinea Pigs; D007150:Immunohistochemistry; D007422:Intestines / Q000294:innervation; D007668:Kidney / Q000294:innervation; D008297:Male; D008643:Mesentery / Q000294:innervation; D009474:Neurons / Q000378:metabolism*; D009478:Neuropeptide Y / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D013004:Somatostatin / Q000378:metabolism*; D013153:Splanchnic Nerves / Q000502:physiology; D013154:Spleen / Q000294:innervation; D014660:Vasoactive Intestinal Peptide / Q000378:metabolism*
D009478:Neuropeptide Y; D014660:Vasoactive Intestinal Peptide; D013004:Somatostatin