Analysis of DNA restriction fragment length polymorphism extends the evidence for breast milk transmission in Streptococcus agalactiae late-onset neonatal infection.
Analysis of restriction fragment length polymorphism (RFLP) of total DNA and of ribosomal DNA (ribotyping) was used to document four cases of Streptococcus agalactiae mother-to-infant transmission potentially associated with ingestion of infected mother's milk. Twenty strains were analyzed. Ten strains were mother-baby pairs, five from the milk of five mothers, four from their neonates with late-onset infection, and one from a colonized neonate. All mothers had early postpartum mastitis. Ten unrelated strains were studied for comparison. In each case, the two strains of each mother-baby pair produced identical RFLP patterns of total DNA. The 10 unrelated strains generated 10 different patterns, one of which, though, was observed in one of the mother-baby pairs. Ribotyping was less discriminative than total DNA RFLP analysis (6 different patterns vs. 13). These data extend the evidence for breast milk transmission in S. agalactiae late-onset neonatal infection.
Bingen
E
E
Laboratoire de Bactériologie and Biochimie Génétique, Hôpital Robert Debré, Paris, France.
Denamur
E
E
Lambert-Zechovsky
N
N
Aujard
Y
Y
Brahimi
N
N
Geslin
P
P
Elion
J
J
D001942:Breast Feeding; D004269:DNA, Bacterial / Q000032:analysis*; D004275:DNA, Ribosomal / Q000032:analysis; D005260:Female; D006801:Humans; D007231:Infant, Newborn; D008413:Mastitis / Q000382:microbiology; D008895:Milk, Human / Q000382:microbiology*; D012150:Polymorphism, Restriction Fragment Length; D011247:Pregnancy; D013290:Streptococcal Infections / Q000382:microbiology / Q000635:transmission*; D013292:Streptococcus agalactiae / Q000145:classification / Q000235:genetics*
D004269:DNA, Bacterial; D004275:DNA, Ribosomal