Quantitative reconstitution of isolated influenza haemagglutinin into liposomes by the detergent method and the immunogenicity of haemagglutinin liposomes.
The reconstitution of influenza virus haemagglutinin into liposomes from lipid/protein/detergent mixtures by detergent removal provides vesicles that are similar in structure to viral particles. The dissociation properties of haemagglutinin aggregates and the molar ratio of lipid to protein in the starting mixture are the key factors for the individual and total yield of protein incorporation into liposomes. Structural properties of the detergent used as well as special reconstitution conditions are of minor importance for the formation of haemagglutinin liposomes. As determined by radial immunodiffusion-, haemolysis- and fusion experiments, specific properties of haemagglutinin were maintained to a large extent on liposomal incorporation, but its immunogenicity is increased, if the antigen is incorporated into the lipid bilayer of liposomes.
Stahn
R
R
Max Delbrück Center for Molecular Medicine, Berlin, Germany.
Schäfer
H
H
Kunze
M
M
Malur
J
J
Ladhoff
A
A
Lachmann
U
U
D000818:Animals; D003902:Detergents; D006389:Hemagglutinins, Viral / Q000737:chemistry* / Q000276:immunology; D008055:Lipids / Q000737:chemistry; D008081:Liposomes / Q000737:chemistry*; D051379:Mice; D008813:Mice, Inbred ICR; D009975:Orthomyxoviridae; D011506:Proteins / Q000737:chemistry
D003902:Detergents; D006389:Hemagglutinins, Viral; D008055:Lipids; D008081:Liposomes; D011506:Proteins