The effect of silibinin (Legalon) on the the free radical scavenger mechanisms of human erythrocytes in vitro.
The effect of Legalon was investigated parallel with that of Adriblastina (doxorubicin) and paracetamol on some parameters characterizing the free radical scavenger mechanisms of human erythrocytes in vitro and on the time of acid hemolysis performed in aggregometer. Observations suggest that Adriblastina enhances the lipid peroxidation of the membrane of red blood cells, while paracetamol causes significant depletion of intracellular glutathione level, thus decreasing the free radical eliminating capacity of the glutathione peroxidase system. Legalon on the other hand, is able to increase the activity of both superoxide dismutase and glutathione peroxidase, which may explain the protective effect of the drug against free radicals and also the stabilizing effect on the red blood cell membrane, shown by the increase of the time of full haemolysis.
Altorjay
I
I
2nd Department of Medicine, University Medical School, Debrecen, Hungary.
Dalmi
L
L
Sári
B
B
Imre
S
S
Balla
G
G
D000082:Acetaminophen / Q000494:pharmacology; D000328:Adult; D004317:Doxorubicin / Q000494:pharmacology; D004912:Erythrocytes / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D016166:Free Radical Scavengers; D005978:Glutathione / Q000097:blood; D005979:Glutathione Peroxidase / Q000097:blood; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D015227:Lipid Peroxidation / Q000187:drug effects; D008315:Malondialdehyde / Q000097:blood; D012838:Silymarin / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000097:blood
D016166:Free Radical Scavengers; D012838:Silymarin; D000082:Acetaminophen; D008315:Malondialdehyde; D004317:Doxorubicin; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005978:Glutathione