Prenatal identification of small mosaic markers of different chromosomal origins.
In situ hybridization using a series of alphoid DNA probes has demonstrated the origin of two small accessory mosaic marker chromosomes ascertained from 1079 amniocenteses. These markers appeared to be de novo, derived from acrocentric chromosomes, and identical by traditional cytogenetic staining (G, Q, C, AgNOR, Hoechst-distamycin). Molecular characterization showed that one marker had originated from chromosome 14, the other from chromosome 22. Clinical outcome in both cases was normal.
Department of Gynecology and Obstetrics, Johns Hopkins University School of Medicine, Baltimore, MD.
G
G
Stetten
K J
KJ
Blakemore
A M
AM
Courter
C A
CA
Coss
E W
EW
Jabs
D000328:Adult; D000650:Amnion / Q000166:cytology; D002503:Centromere; D002883:Chromosomes, Human, Pair 14; D002892:Chromosomes, Human, Pair 22; D015342:DNA Probes; D005260:Female; D005819:Genetic Markers; D006801:Humans; D007621:Karyotyping; D009030:Mosaicism; D009693:Nucleic Acid Hybridization; D011296:Prenatal Diagnosis / Q000379:methods*; D013194:Staining and Labeling
D015342:DNA Probes; D005819:Genetic Markers