Isolation of delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine from culture broths by covalent chromatography.
An efficient procedure for the isolation of reduced delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine (ACV) from culture supernatants of beta-lactam antibiotic-producing microorganisms is described. The method utilises covalent chromatography to isolate thiols from culture broths that have been deproteinised and undergone borohydride reduction. 2-Pyridyl disulphide activated thiopropyl Sepharose was employed batchwise to isolate the thiols present in such broths from cultures of the known ACV excreter Cephalosporium acremonium N2 and the penicillin producer Penicillium chrysogenum P2. ACV was separated from these mixtures of thiols by gel permeation chromatography. Reversed-phase HPLC analysis showed the ACV to be of a high purity unless isolated from a highly complex culture medium.
School of Biological and Health Sciences, Division of Biotechnology, Polytechnic of Central London, UK.
C D
CD
Orford
M W
MW
Adlard
D
D
Perry
D000164:Acremonium / Q000032:analysis*; D000595:Amino Acid Sequence; D002845:Chromatography / Q000379:methods*; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000302:isolation & purification*; D010408:Penicillium chrysogenum / Q000032:analysis*; D012685:Sepharose / Q000031:analogs & derivatives
D009842:Oligopeptides; C011638:5-(2-aminoadipyl)cysteinylvaline; C035872:thiopropyl-sepharose; D012685:Sepharose