Effects of activin A and somatostatin on intact FSH secretion and intracellular Ca2+ concentration in human FSH-secreting pituitary adenoma cells.
Activin A stimulated synthesis and secretion of intact FSH in dispersed human FSH-secreting adenoma cells. Significant stimulation was observed after 24 hr. Activin A caused an increase in Ca2+ concentration ([Ca2+]i). This response occurred soon after the activin A action. These effects were blocked in Ca(2+)-deficient medium and by nitrendipine (5 microM). Somatostatin inhibited the activin A-induced intact FSH secretion and the [Ca2+]i response. These findings indicated that Ca2+ influx through voltage-gated Ca2+ channel was involved in the activin A induced synthesis and secretion of intact FSH.
Takano
K
K
Fourth Department of Internal Medicine, University of Tokyo School of Medicine, Japan.
Yamashita
N
N
Kojima
I
I
Kitaoka
M
M
Teramoto
A
A
Ogata
E
E
D028341:Activins; D000236:Adenoma / Q000378:metabolism* / Q000601:surgery; D002118:Calcium / Q000378:metabolism* / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005640:Follicle Stimulating Hormone / Q000096:biosynthesis / Q000097:blood / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D007265:Inhibins / Q000494:pharmacology*; D007700:Kinetics; D009568:Nitrendipine / Q000494:pharmacology; D010911:Pituitary Neoplasms / Q000378:metabolism* / Q000601:surgery; D013004:Somatostatin / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D028341:Activins; D013004:Somatostatin; D007265:Inhibins; D005640:Follicle Stimulating Hormone; D009568:Nitrendipine; D002118:Calcium