Studies on novel pili from Shigella flexneri. I. Detection of pili and hemagglutination activity.
Pili were detected using electron microscopy in clinical isolates of Shigella flexneri which had been continuously subcultivated in liquid media. Morphologically, the pili appeared as thin, flexible, cylindrical structures of up to 2-5 microns in length and about 3-5 nm in diameter. Two strains showed mannose-resistant (MR) hemagglutination to fresh fowl erythrocytes (type 4), and one to tannic acid-treated horse erythrocyte (type 3) pili. These pili are novel and different from the mannose-sensitive (MS) type 1 pili described by Duguid and Gillies.
Department of Bacteriology, Nagasaki University, Japan.
A
A
Utsunomiya
T
T
Naito
M
M
Ehara
Y
Y
Ichinose
A
A
Hamamoto
D000818:Animals; D003470:Culture Media / Q000494:pharmacology; D004912:Erythrocytes / Q000276:immunology; D010861:Fimbriae, Bacterial / Q000187:drug effects / Q000276:immunology* / Q000648:ultrastructure*; D006384:Hemagglutination / Q000187:drug effects; D006386:Hemagglutination Tests; D006801:Humans; D008358:Mannose / Q000494:pharmacology; D012763:Shigella flexneri / Q000187:drug effects / Q000276:immunology* / Q000648:ultrastructure*; D013997:Time Factors
D003470:Culture Media; D008358:Mannose