A single 24h contact time with adriamycin provokes the emergence of resistant cells expressing the Gp 170 protein.
We investigate here, in A549 cells, the influence of a single short (1h) or long (24h) adriamycin (ADR) contact time on the long-term cytotoxicity of the drug and on the emergence of resistant cells. In contrast to a 1 h ADR contact, a 24 h treatment provokes the emergence of resistant cells overexpressing the Gp170 protein and this overexpression is maintained for at least three months without any drug selection pressure.
Institut Curie, Section de Biologie, Paris, France.
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S
Chevillard
P
P
Vielh
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G
Bastian
J
J
Coppey
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D002460:Cell Line; D004317:Doxorubicin / Q000494:pharmacology*; D004351:Drug Resistance; D006801:Humans; D008562:Membrane Glycoproteins / Q000032:analysis*; D013997:Time Factors
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D008562:Membrane Glycoproteins; D004317:Doxorubicin