Imidazole-SDS-Zn reverse staining of proteins in gels containing or not SDS and microsequence of individual unmodified electroblotted proteins.
A reverse staining procedure is described for the detection of proteins in acrylamide and agarose gels with and without SDS. Protein detection occurs a few minutes after electrophoresis. The sensitivity on acrylamide gels is higher than that of Coomassie blue staining either on acrylamide gels or on electrotransferred membranes. Sequencing of protein bands only detected by reverse staining on the gel and not by Coomassie blue is demonstrated.
Servicio de Endocrinologia, Hospital Ramon y Cajal, Madrid, Spain.
M L
ML
Ortiz
M
M
Calero
C
C
Fernandez Patron
C F
CF
Patron
L
L
Castellanos
E
E
Mendez
D004587:Electrophoresis, Agar Gel / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D007093:Imidazoles; D011506:Proteins / Q000032:analysis*; D012967:Sodium Dodecyl Sulfate; D013194:Staining and Labeling / Q000379:methods*; D015032:Zinc
D007093:Imidazoles; D011506:Proteins; D012967:Sodium Dodecyl Sulfate; C029899:imidazole; D015032:Zinc