Chronic intrajejunal TNBS application in TNBS-sensitized rats: a new model of chronic inflammatory bowel diseases.
An enteritis, based on a delayed-type hypersensitivity reaction, was induced in TNBS (2,4,4-trinitrobenzenesulfonic acid) sensitized rats by intrajejunal challenge with TNBS. This treatment induced a chronic inflammation of the distal small intestine, characterized by gross hyperaemia and oedema, as assessed by a macroscopic score. Histologically, the inflammatory response included cell infiltration by lymphocytes and histiocytes, a transmural granulomatous inflammation with multinucleated cells and activated mesenteric lymph nodes. Drug treatment with sulfasalazine or 5-aminosalicylic acid improved enteritis score. The applicability and relevance of this new model is discussed in relation to drug development and basic research of inflammatory bowel diseases.
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Department of Pharmacology, Center of Research, Grünenthal GmbH, Aachen, FRG.
D000636:Aminosalicylic Acids / Q000008:administration & dosage / Q000494:pharmacology; D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D007114:Immunization; D015212:Inflammatory Bowel Diseases / Q000139:chemically induced / Q000188:drug therapy / Q000473:pathology; D007413:Intestinal Mucosa / Q000187:drug effects* / Q000473:pathology; D007583:Jejunum; D019804:Mesalamine; D051381:Rats; D017207:Rats, Sprague-Dawley; D012460:Sulfasalazine / Q000008:administration & dosage / Q000494:pharmacology; D014302:Trinitrobenzenesulfonic Acid / Q000008:administration & dosage / Q000276:immunology / Q000633:toxicity*
D000636:Aminosalicylic Acids; D012460:Sulfasalazine; D019804:Mesalamine; D014302:Trinitrobenzenesulfonic Acid