Engineering of proteases and protease inhibition.
Proteases are unquestionably the single most studied class of enzymes and yet many questions still remain about their mechanisms and roles. Protein engineering offers the opportunity to provide some of the answers. In this review, recent advances towards the understanding of stability, mechanism, specificity and regulation of proteases and their inhibitors are outlined. In addition, the application of this increased understanding is also discussed.
National Research Council, Biotechnology Research Institute, Montréal, Québec, Canada.
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Storer
D000818:Animals; D010450:Endopeptidases / Q000737:chemistry* / Q000378:metabolism; D004795:Enzyme Stability; D006801:Humans; D011480:Protease Inhibitors / Q000737:chemistry*; D015202:Protein Engineering; D013379:Substrate Specificity
D011480:Protease Inhibitors; D010450:Endopeptidases