Evidence for oligomannosyl residues containing both beta-1,2 and alpha-1,2 linkages as a serotype A-specific epitope(s) in mannans of Candida albicans.
In order to identify the branches containing both beta-1,2 and alpha-1,2 linkages as the serotype A-specific epitope(s) in the mannans of Candida albicans, serotype A strains with oligosaccharides constituting the beta-1,2 linkage, the alpha-1,2 linkage, and both the beta-1,2 and the alpha-1,2 linkages were prepared from the mannans of C. albicans serotype A strains (NIH A-207 and J-1012) and tested for their inhibitory effects in the precipitin and slide agglutination assays. The results indicated that two oligosaccharides containing both beta-1,2 and alpha-1,2 linkages, Manp beta 1-2Manp alpha 1-2Manp alpha 1-2Manp alpha 1-2Man and Manp beta 1-2Manp beta 1-2Manp alpha 1-2Manp alpha 1-2Manp alpha 1-2Man, served as epitopes participating in the serotype A specificity of C. albicans strains.
Second Department of Hygienic Chemistry, Tohoku College of Pharmacy, Miyagi, Japan.
H
H
Kobayashi
N
N
Shibata
S
S
Suzuki
D000371:Agglutination; D002176:Candida albicans / Q000276:immunology*; D002240:Carbohydrate Sequence; D000939:Epitopes / Q000032:analysis*; D008351:Mannans / Q000276:immunology*; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000276:immunology*; D012703:Serotyping
D000939:Epitopes; D008351:Mannans; D009844:Oligosaccharides