Fractionation of gamma-glutamyl transpeptidase from the rat brain, kidney and pancreas by concanavalin A affinity chromatography.
gamma-Glutamyl transpeptidase (GGT) in tissue extracts from five brain regions, the kidney and pancreas of 50-day-old rats was examined for its specific activity and affinity to immobilized concanavalin A (Con A). According to their descending GGT activity, the tissue extracts were classified in the following order: kidney >> pancreas >> olfactory bulbs > medulla oblongata > hippocampus > cerebellum > frontal cortex. Using different concentrations of methyl-alpha-D-mannopyranoside for the elution of GGT from Con A-Sepharose column, the enzyme from brain regions could be separated into five fractions, two of which contained about 75% of the total GGT activity without regional differences in elution profiles. Almost complete GGT activity in kidney tissue extracts was eluted in a single peak whereas the enzyme from pancreas exhibited two peaks.
Dvoráková
L
L
Institute of Physiology, Czechoslovak Academy of Sciences, Prague.
Lisý
V
V
Stastný
F
F
D000818:Animals; D001921:Brain / Q000201:enzymology*; D005591:Chemical Fractionation; D002846:Chromatography, Affinity; D003208:Concanavalin A / Q000737:chemistry; D005260:Female; D007668:Kidney / Q000737:chemistry / Q000201:enzymology*; D010179:Pancreas / Q000737:chemistry / Q000201:enzymology*; D051381:Rats; D017208:Rats, Wistar; D014020:Tissue Extracts; D005723:gamma-Glutamyltransferase / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*
D014020:Tissue Extracts; D003208:Concanavalin A; D005723:gamma-Glutamyltransferase