Inhibitory effect of a Microcystis sp (cyanobacteria) toxin on development of preimplantation mouse embryos.
1. A soluble toxin, purified from the algae bloom of an eutrophic lake dominated by Microcystis, is a very effective inhibitor of early embryo development in a dose-response relationship. 2. Two- and 8-cell mouse embryos under the influence of Microcystis toxin do not reach the developmental stages of morula and blastocyst, respectively. 3. Actin cortex is disorganized without change in the microtubules structure. 4. Results are discussed in terms of the possible mechanisms by which the toxin arrests development considering, specifically, effects on the cytoskeleton and/or on voltage-insensitive transmembrane Ca2+ channels.
Sepulveda
M S
MS
Departamento de Biologia, Facultad de Ciencias, Universidad de Chile.
Rojas
M
M
Zambrano
F
F
D000818:Animals; D001755:Blastocyst / Q000187:drug effects* / Q000648:ultrastructure; D046508:Culture Techniques; D005314:Embryonic and Fetal Development / Q000187:drug effects*; D051379:Mice; D046931:Microcystis / Q000378:metabolism*; D008854:Microscopy, Electron; D014118:Toxins, Biological / Q000633:toxicity*
D014118:Toxins, Biological