A novel and efficient method for enzymatic synthesis of high purity maltose using moranoline (1-deoxynojirimycin).
A transglycosylation reaction with moranoline (1-deoxynojirimycin) was done with soluble starch as the glucosyl donor and Bacillus macerans amylase as a cyclodextrin glycosyltransferase [EC 2.4.1.19]. The resultant transglycosylation products with moranoline, obtained by treating the reaction mixture with a strong cation exchange resin, were hydrolyzed by beta-amylase [EC 3.2.1.2] from sweet potatoes. The hydrolysate was treated with a strong cation exchange resin, and high purity maltose was obtained.
Chemistry Laboratories, Nippon Shinyaku Co., Ltd., Kyoto, Japan.
S
S
Maruo
H
H
Yamashita
K
K
Miyazaki
H
H
Yamamoto
Y
Y
Kyotani
H
H
Ogawa
M
M
Kojima
Y
Y
Ezure
D017485:1-Deoxynojirimycin / Q000378:metabolism*; D002240:Carbohydrate Sequence; D002411:Cation Exchange Resins; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D003912:Dextrins / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D005964:Glucosyltransferases / Q000378:metabolism*; D006031:Glycosylation; D009682:Magnetic Resonance Spectroscopy; D008320:Maltose / Q000138:chemical synthesis* / Q000302:isolation & purification; D008722:Methods; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000378:metabolism; D013213:Starch / Q000378:metabolism; D001614:beta-Amylase / Q000378:metabolism*
D002411:Cation Exchange Resins; D003912:Dextrins; D009844:Oligosaccharides; D017485:1-Deoxynojirimycin; D008320:Maltose; D013213:Starch; D005964:Glucosyltransferases; C019963:cyclomaltodextrin glucanotransferase; D001614:beta-Amylase; D005947:Glucose