Structures of sugar chains of water-soluble glycoproteins in developing castor bean cotyledons.
The structures of sugar chains from the water-soluble glycoproteins in developing castor beans have been identified. The structural analyses were done by a fluorescence method combined with exoglycosidase digestions and 1H-NMR spectroscopy. The identified structures fell into two categories; one was an oligomannose-type, the other xylomannose-type or xylose-containing type. Among these oligosaccharides, Man3Fuc1Xyl1GlcNAc2 (M3FX; 38%) and Man6GlcNAc2 (M6B; 22%) were the major structures. The higher mannose-content oligosaccharides (Man8-7GlcNAc2) were only 4.1%, and the further-modified structures (GNM3FX, M2FX) than M3FX were 22% of the total.
Department of Agricultural Sciences, Faculty of Agriculture, Okayama University, Japan.
Y
Y
Kimura
H
H
Suehisa
O
O
Yamaguchi
S
S
Nakajima
S
S
Takagi
D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000737:chemistry*; D002367:Ricinus communis / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D005959:Glucosidases; D006023:Glycoproteins / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000737:chemistry*; D010947:Plants, Toxic; D013050:Spectrometry, Fluorescence
D002241:Carbohydrates; D006023:Glycoproteins; D010940:Plant Proteins; D005959:Glucosidases