Are Sinc and the PrP gene congruent? Evidence from PrP gene analysis in Sinc congenic mice.
Congenic mouse strains VM/Dk and VM-Sincs7/Dk differ at the Sinc gene, which controls the incubation period of scrapie in mice; VM/Dk mice are Sincp7p7 and VM-Sincs7/Dk mice are Sincs7s7. Restriction fragment length polymorphism and DNA sequencing analysis demonstrated that the PrP genes also differ in these strains, confirming the close genetic linkage of Sinc and PrP. Using the restriction enzyme HhaI, we have shown that at least 100 kb of DNA flanking the PrP gene differs between the two strains, and therefore the congruence of the Sinc and PrP genes is still not certain.
AFRC Institute for Animal Health, AFRC/MRC Neuropathogenesis Unit, Edinburgh, U.K.
N
N
Hunter
J C
JC
Dann
A D
AD
Bennett
R A
RA
Somerville
I
I
McConnell
J
J
Hope
D000818:Animals; D001923:Brain Chemistry; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D005796:Genes / Q000235:genetics; D051379:Mice; D008815:Mice, Inbred Strains / Q000235:genetics* / Q000382:microbiology; D009419:Nerve Tissue Proteins / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D018620:PrPSc Proteins; D011328:Prions / Q000235:genetics*; D015183:Restriction Mapping; D012376:Rodent Diseases / Q000235:genetics* / Q000382:microbiology; D012608:Scrapie / Q000235:genetics*; D017422:Sequence Analysis, DNA; D013997:Time Factors
D009419:Nerve Tissue Proteins; D018620:PrPSc Proteins; D011328:Prions; D015252:Deoxyribonucleases, Type II Site-Specific; C037130:GCGC-specific type II deoxyribonucleases