Gene transfer to tumor-infiltrating lymphocytes and other mammalian somatic cells by microprojectile bombardment.
We demonstrate the application of particle delivery to the transformation of mammalian somatic cells. Two mouse T-lymphocyte cell lines and Chinese hamster ovary (CHO) cells were transformed transiently with the RSV-ADH (alcohol dehydrogenase) gene. Stable gene transfer was also demonstrated in CHO cells at a frequency of 6 x 10(-4) and in T-cells at a frequency of 1 x 10(-4), using beta-Gal/neomycin-resistance gene constructs. The helium gas acceleration mechanism (PDS 1000/He) and particle delivery method allowed better velocity control and particle dispersion than the gunpowder driven instrument. These improvements have increased cell viability, transformation efficiency, and cellular targets.
Fitzpatrick-McElligott
S
S
DCV DuPont/ConAgra, Wilmington, DE 19810.
D000399:Air Pressure; D000818:Animals; D016466:CHO Cells; D002460:Cell Line; D002470:Cell Survival; D006224:Cricetinae; D015870:Gene Expression; D005819:Genetic Markers; D016246:Lymphocytes, Tumor-Infiltrating / Q000378:metabolism; D051379:Mice; D014162:Transfection / Q000379:methods*; D014170:Transformation, Genetic
D005819:Genetic Markers