Hydrolysis of concentrated sucrose syrups by invertase immobilized on anion exchanger waste cotton thread.
Yeast invertase was immobilized on polyethyleneimine-coated cotton thread by adsorption followed by crosslinking with glutaraldehyde. The thread-bound invertase was used as an easily retrievable system for the hydrolysis of 80% w/v commercial sucrose syrups. The immobilized enzyme was stable for over 90 days to a temperature of 50 degrees C, only when stored in 80% sucrose solution. Above this temperature, inactivation of enzyme was observed. The cotton threads were used in a batch reactor for hydrolysis of sucrose in about 30 batches carried out over a period of 50 days without loss in activity. The threads could also be used in a packed bed reactor (1.51) for 97% hydrolysis of 80% sucrose syrups at 50 degrees C at a rate of about 360 kg per month for a period of 3 months.
Food Technology and Enzyme Engineering Division, Bhabha Atomic Research Centre, Bombay, India.
S S
SS
Godbole
B S
BS
Kubal
S F
SF
D'Souza
D000838:Anions; D004800:Enzymes, Immobilized / Q000378:metabolism*; D006026:Glycoside Hydrolases / Q000378:metabolism*; D003368:Gossypium; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007700:Kinetics; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D013395:Sucrose / Q000378:metabolism*; D043324:beta-Fructofuranosidase
D000838:Anions; D004800:Enzymes, Immobilized; D013395:Sucrose; D006026:Glycoside Hydrolases; D043324:beta-Fructofuranosidase