Vitronectin diversity in evolution but uniformity in ligand binding and size of the core polypeptide.
We isolated vitronectins from the plasma or sera of 14 animal species including mouse and rat by heparin affinity chromatography. They cross-reacted with anti-vitronectin antibody and their amino terminal sequences showed strong homology. They also promoted spreading of BHK cells and were bound to heparin and collagen in the same way. Therefore, these properties appear to be essential for vitronectin function. However, the apparent molecular weights of these vitronectins varied considerable from 59 to 78 kDa in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). In addition, the number of bands also varied from 1 to 3. To search for the uniformity of vitronectin polypeptide, vitronectins were deglycosylated and examined by Ferguson plot analysis. The size of the polypeptide portion of vitronectins was estimated to range from 40 to 57 kDa which was 19-26 kDa smaller than original values. Supposing a possible cleavage site at 5-13 kDa far from the carboxyl terminus, all vitronectin polypeptides were speculated to be synthesized de novo in the size range of 50-57 kDa. Proteins reacting with anti-vitronectin antibody were also detected on the immunoblot of 13 more species including Drosophila and Physarum. Almost all of these vitronectin-like proteins showed marked species-specific variations in their apparent molecular weights from 51 to 96 kDa in SDS-PAGE.
Department of Biology, Faculty of Science, Ochanomizu University, Tokyo, Japan.
N
N
Nakashima
K
K
Miyazaki
M
M
Ishikawa
T
T
Yatohgo
H
H
Ogawa
H
H
Uchibori
I
I
Matsumoto
N
N
Seno
M
M
Hayashi
D000595:Amino Acid Sequence; D000818:Animals; D005075:Biological Evolution; D002417:Cattle; D002460:Cell Line / Q000737:chemistry; D002645:Chickens; D006224:Cricetinae; D004285:Dogs; D006023:Glycoproteins / Q000097:blood / Q000302:isolation & purification*; D006031:Glycosylation; D006168:Guinea Pigs; D006196:Hagfishes; D006801:Humans; D051379:Mice; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011817:Rabbits; D051381:Rats; D013552:Swine; D019096:Vitronectin
D006023:Glycoproteins; D019096:Vitronectin