Isolation and mapping of 88 new RFLP markers on human chromosome 8.
To obtain new RFLP markers for construction of a high-resolution map of human chromosome 8, a cosmid library was constructed from a somatic hybrid cell that contained chromosome 8 as the only human component in mouse genomic background. Eighty-eight new RFLP markers were isolated and characterized, and 71 of them were sublocalized to chromosomal bands by fluorescent in situ hybridization (FISH). Of these, 36 were localized to the short arm, 34 to the long arm, and 1 to the centromeric region. Five markers defined VNTR loci. This work represents the first extensive isolation and physical mapping of RFLP markers on human chromosome 8. These new markers will serve as useful resources for linkage mapping of loci for inherited diseases and for efforts to identify a putative tumor suppressor gene(s) on chromosome 8.
Emi
M
M
Division of Biochemistry, Cancer Institute, Tokyo, Japan.
Takahashi
E
E
Koyama
K
K
Okui
K
K
Oshimura
M
M
Nakamura
Y
Y
D001345:Autoradiography; D015139:Blotting, Southern; D002874:Chromosome Mapping; D002898:Chromosomes, Human, Pair 8; D003360:Cosmids; D004247:DNA; D005453:Fluorescence; D005819:Genetic Markers; D006801:Humans; D007621:Karyotyping; D009693:Nucleic Acid Hybridization; D012150:Polymorphism, Restriction Fragment Length
D005819:Genetic Markers; D004247:DNA