Developmental changes in the effect of atrial natriuretic peptide on tissue cyclic GMP content and particulate guanylate cyclase activity of aorta, kidney and lung of rats.
To investigate possible developmental changes in the physiological effect of atrial natriuretic peptide (ANP) after birth, we studied the effect of ANP on the slice cGMP content and the particulate guanylate cyclase activity of aorta, kidney and lung in neonate, 2-week-old and adult rats of both sexes. Incubation with human ANP(99-126) (hANP) increased significantly the slice cGMP content of aorta, kidney and lung in three ages of rats. The hANP-stimulated fraction of cGMP contents of kidney decreased, that of lung increased with development, whereas that of aorta showed no significant change. Consistently, the hANP-responsive particulate guanylate cyclase activity decreased in kidney, increased in lung during development, without significant developmental change in aorta. These results indicate a differential change in the effect of hANP on the slice cGMP content among tissues during development. The developmental change in the effect of hANP on slice cGMP content is probably caused by the ontogenetic change in activation of ANP receptor-linked guanylate cyclase.
Department of Pharmacology, Tokyo Women's Medical College, Japan.
T
T
Muraki
K
K
Irie
Y
Y
Uchida
T
T
Nomoto
D000375:Aging; D000818:Animals; D001011:Aorta / Q000201:enzymology / Q000254:growth & development; D009320:Atrial Natriuretic Factor / Q000494:pharmacology*; D006152:Cyclic GMP / Q000378:metabolism*; D005260:Female; D006162:Guanylate Cyclase / Q000378:metabolism*; D007668:Kidney / Q000201:enzymology / Q000254:growth & development; D008168:Lung / Q000201:enzymology / Q000254:growth & development; D008297:Male; D011863:Radioimmunoassay; D051381:Rats; D017208:Rats, Wistar
D009320:Atrial Natriuretic Factor; D006162:Guanylate Cyclase; D006152:Cyclic GMP