Media additives for protecting freely suspended animal cells against agitation and aeration damage.
Over the past 30 years, several different additives have been used to protect freely suspended animal cells in culture from agitation and aeration damage. These include pluronic polyols, various derivatized celluloses and starches, protein mixtures, polyvinyl-pyrrolidones, dextrans, and, more recently, polyethylene glycol (PEG) and polyvinyl alcohol. The protective mechanisms of these additives are not yet fully understood, but recent studies suggest both fluid-mechanical and biological mechanisms of protection.
Department of Chemical Engineering, Northwestern University, Evanston, IL 60208-3120.
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Papoutsakis
D000818:Animals; D001709:Biotechnology; D002469:Cell Separation; D003470:Culture Media; D003584:Cytological Techniques; D066298:In Vitro Techniques; D011108:Polymers
D003470:Culture Media; D011108:Polymers