Taurine uptake into chick B cells.
The objective of the present study was to determine whether chick B cells possess a specific transport system for taurine. The Bursa of Fabricius was isolated from newly hatched to 6-week-old chicks and an enriched fraction (86.2%) of B cells was isolated. The chick B cells maintained a high intracellular taurine concentration (0.8-1.12 mM) that decreased with age. The B cells exhibited carrier-mediated and simple diffusion uptake components, but only the carrier-mediated component increased with age. Inhibitor studies indicated taurine uptake was sodium and energy dependent. The data demonstrate that chick B cells possess a specialized taurine transport system and the activity of this system changes during posthatch B cell development.
Division of Animal and Veterinary Sciences, College of Agriculture and Forestry, West Virginia University, Morgantown 26506.
D W
DW
Porter
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WG
Martin
D000375:Aging / Q000276:immunology*; D000704:Analysis of Variance; D000818:Animals; D000831:Animals, Newborn; D001402:B-Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D001692:Biological Transport / Q000187:drug effects; D002060:Bursa of Fabricius / Q000378:metabolism; D002645:Chickens / Q000276:immunology*; D002851:Chromatography, High Pressure Liquid; D003545:Cysteine / Q000031:analogs & derivatives / Q000494:pharmacology; D004140:Dinitrophenols / Q000494:pharmacology; D018377:Neurotransmitter Agents / Q000494:pharmacology; D010042:Ouabain / Q000494:pharmacology; D012964:Sodium / Q000494:pharmacology; D013654:Taurine / Q000493:pharmacokinetics*; D015091:beta-Alanine / Q000494:pharmacology
D004140:Dinitrophenols; D018377:Neurotransmitter Agents; D015091:beta-Alanine; D013654:Taurine; D010042:Ouabain; D012964:Sodium; D003545:Cysteine; C013461:cysteine sulfinic acid