Gene expression of insulin-like growth factor-I and IGF-I receptor during wound healing in rats.
OBJECTIVE
To measure the time course of changes in the concentrations of insulin-like growth factor-I (IGF-I), IGF-I messenger RNA (mRNA), and IGF-I receptor mRNA during wound healing.


METHODS
Open experimental study.


METHODS
40 male Sprague-Dawley rats, each weighing 300 g.


METHODS
Stainless steel wire mesh cylinders implanted in the subcutaneous tissue of the back. Five rats killed at each time point (1.5, 2, 3, 4, 5, 7, 11, and 18 weeks).


METHODS
Wet weight and concentrations of IGF-I mRNA, and IGF-I receptor mRNA of granulation tissue, and concentration of IGF-I in wound fluid.


RESULTS
The wet weight of granulation tissue increased significantly between week 1.5 and weeks 4-5, and then decreased. IGF-I mRNA concentration (amol/microgram DNA) increased significantly (threefold) between week 1.5 and weeks 3-5, and decreased between weeks 5 and 7. The concentration of IGF-I receptor RNA remained constant throughout the study, and the concentration of IGF-I in wound fluid remained constant until week 8 and then increased to a higher level at weeks 11-18.


CONCLUSIONS
There is a transient rise in the gene expression of IGF-I but not of IGF-I receptor mRNA during wound healing.
Steenfos
H H
HH
Department of Plastic Surgery, University of Göteborg, Sweden.
Jansson
J O
JO
D000818:Animals; D015870:Gene Expression; D007334:Insulin-Like Growth Factor I / Q000032:analysis / Q000096:biosynthesis* / Q000502:physiology; D008297:Male; D012333:RNA, Messenger / Q000032:analysis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017526:Receptor, IGF Type 1 / Q000096:biosynthesis*; D014945:Wound Healing / Q000235:genetics / Q000502:physiology*
D012333:RNA, Messenger; D007334:Insulin-Like Growth Factor I; D017526:Receptor, IGF Type 1