The genetic stability of Penicillium chrysogenum transformants in a fermentor.
A number of transformants of Penicillium chrysogenum have been obtained with the plasmid vector p3SR2. Southern analysis showed that transformation had occurred by integration of vector sequences into the nuclear DNA of the fungus. A number of transformants were tested for stability of the transformed phenotype in agar medium and some were found to be unstable. Two transformants, shown to be stable in agar culture, were grown in 5-1 batch fermentors for further stability tests. Over periods of up to 312 h in the fermentor both transformants were 100% stable with respect to the transformed phenotype. In addition Southern analysis of DNA extracted from the spent mycelium showed that no change had occurred in the position of the integrated vector sequences within the transformant nuclear DNA.
School of Biotechnology, Polytechnic of Central London, UK.
D V
DV
Renno
G
G
Saunders
A T
AT
Bull
G
G
Holt
D015139:Blotting, Southern; D003470:Culture Media; D004271:DNA, Fungal / Q000032:analysis; D005285:Fermentation / Q000235:genetics; D010408:Penicillium chrysogenum / Q000235:genetics*; D014170:Transformation, Genetic
D003470:Culture Media; D004271:DNA, Fungal