Construction of a umuDC operon substitution mutation in Escherichia coli.
Using a specialized transducing lambda phage, the umuDC operon of Escherichia coli was deleted and replaced with the chloramphenicol acetyltransferase gene. The delta (umuDC)595::cat mutation was subsequently transferred by generalized P1 transduction into a variety of genetic backgrounds. It is concluded that the UmuDC proteins, which are normally required for inducible mutagenesis, are not essential for cell survival.
Woodgate
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Section on Viruses and Cellular Biology, National Institute of Child Health and Human Development, Bethesda, MD 20892.
D010582:Bacteriophage lambda / Q000235:genetics; D015139:Blotting, Southern; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics*; D002872:Chromosome Deletion; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D008957:Models, Genetic; D009876:Operon; D015183:Restriction Mapping; D014161:Transduction, Genetic
D004269:DNA, Bacterial; D015500:Chloramphenicol O-Acetyltransferase