Physical mapping of a 950-kb region surrounding a locus (D10S102) tightly linked to the MEN2A gene.
We have constructed a long-range contig of cosmid and YAC clones around D10S102, a locus that is tightly linked to the gene responsible for multiple endocrine neoplasia type 2A (MEN2A). With D10S102 as a starting point, a 360-kb cosmid contig was constructed by bidirectional genomic walking, and at least six fragments from these cosmids showed high sequence homology to other species. Five YAC clones were also isolated at the D10S102 locus, and they formed a contig covering 950 kb of genomic DNA. Furthermore, we obtained six RFLP systems from the contig, which will serve as new resources for fine-scale genetic linkage mapping of the MEN2A locus.
Division of Biochemistry, Cancer Institute, Tokyo, Japan.
T
T
Tokino
T
T
Imai
A
A
Tanigami
S
S
Takiguchi
Y
Y
Nakamura
D001483:Base Sequence; D002874:Chromosome Mapping; D016386:Chromosome Walking; D002879:Chromosomes, Human, Pair 10; D003360:Cosmids; D004273:DNA, Neoplasm / Q000235:genetics; D008040:Genetic Linkage; D006801:Humans; D008969:Molecular Sequence Data; D009377:Multiple Endocrine Neoplasia / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D012441:Saccharomyces cerevisiae / Q000235:genetics
D004273:DNA, Neoplasm