The role of the excision and error-prone repair systems in mutagenesis by fluorinated quinolones in Salmonella typhimurium.
Patterns of reversion produced by ciprofloxacin, enoxacin and ofloxacin in Salmonella typhimurium strains carrying the hisG428 ochre mutation have been studied. These fluorinated quinolones produce a significant increase in reversion of this mutation, even when it is located on the chromosome. Nevertheless, reversion is higher when the hisG428 mutation is on the multicopy plasmid pAQ1 than when it is on the chromosome. Reversion of hisG428 induced by fluorinated quinolones is abolished both in a uvrB genetic background and in the absence of the plasmid pKM101. Therefore, mutagenesis produced by fluorinated quinolones in the Salmonella mutagenicity assay is significantly affected by both the excision repair and the error-prone repair systems. Furthermore, fluorinated quinolones are also detected as moderate mutagens with the base substitution hisG46 mutation when both repair systems are functional in the tester strain.
Departament de Genètica i de Microbiologia, Universitat Autònoma de Barcelona, Spain.
B
B
Clerch
J
J
Barbé
M
M
Llagostera
D002939:Ciprofloxacin / Q000494:pharmacology*; D004260:DNA Repair; D004305:Dose-Response Relationship, Drug; D015365:Enoxacin / Q000494:pharmacology*; D005798:Genes, Bacterial; D005838:Genotype; D016296:Mutagenesis; D009152:Mutagenicity Tests; D009153:Mutagens / Q000494:pharmacology*; D015242:Ofloxacin / Q000494:pharmacology*; D012486:Salmonella typhimurium / Q000187:drug effects* / Q000235:genetics
D009153:Mutagens; D015365:Enoxacin; D002939:Ciprofloxacin; D015242:Ofloxacin