An anticoagulant fucoidan from the brown seaweed Ecklonia kurome.
The structure of a alpha-L-fucose-rich, sulphated polysaccharide (C-I) with a potent anticoagulant activity, which was isolated from the brown seaweed Ecklonia kurome, has been studied. Methylation analysis showed that C-I consisted mainly of 3-linked and 3,4-disubstituted fucopyranosyl residues in addition to non-reducing terminal fucofuranosyl and fucopyranosyl residues, 2,3-di- and 2,3,4-tri-substituted fucopyranosyl residues and galactopyranosyl residues with various glycosidic linkages. Methanolysis of C-I gave neutral di-, tri-, tetra- and highly polymerized-oligosaccharide fractions. GC-MS and methylation analysis indicated that di- and trisaccharide fractions consisted mainly of Fuc-(1----3)-Fuc and Fuc-(1----3)-Fuc-(1----3)-Fuc, respectively, in addition to small amounts of Fuc-(1----4)-Fuc, Fuc-(1----4)-Gal and Fuc-(1----3)-[Fuc-(1----2)-]Fuc. When methylated C-I was subjected to methanolysis for desulphation followed by remethylation with deuterated methyl iodide, most of deuteriomethyl groups substituted to position 4 of 3-linked Fuc.
Department of Biophysics, School of Hygienic Sciences, Kitasato University, Kanagawa, Japan.
T
T
Nishino
H
H
Kiyohara
H
H
Yamada
T
T
Nagumo
D000925:Anticoagulants / Q000737:chemistry / Q000302:isolation & purification*; D002240:Carbohydrate Sequence; D008745:Methylation; D008969:Molecular Sequence Data; D000459:Phaeophyceae / Q000032:analysis*; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology
D000925:Anticoagulants; D011134:Polysaccharides; C007789:fucoidan