Characterization of the gene encoding the plastid-located glutamine synthetase of Phaseolus vulgaris: regulation of beta-glucuronidase gene fusions in transgenic tobacco.
The gln-delta gene, which encodes the plastid-located glutamine synthetase of Phaseolus vulgaris, was cloned and its promoter region was sequenced. Primer extension analysis was used to map the two major transcription initiation sites which are about 90 nucleotides apart. A fusion of 2.3 kb of the upstream region of the gln-delta gene to the reporter gene uidA encoding beta-glucuronidase was shown to be expressed in the chlorophyllous cell types of leaves and stems and in the root meristem region of transgenic tobacco. Analysis of a series of three 5' promoter deletion fusions revealed the presence of a region essential for promoter activity between -786 and -327 and regions involved in tissue-specific regulation and light regulation between -786 and +43.
Department of Biological Sciences, University of Warwick, England, UK.
J M
JM
Cock
P
P
Hémon
J V
JV
Cullimore
D001483:Base Sequence; D004252:DNA Mutational Analysis; D007887:Fabaceae / Q000201:enzymology* / Q000235:genetics; D005786:Gene Expression Regulation / Q000502:physiology*; D005966:Glucuronidase / Q000235:genetics / Q000378:metabolism; D005974:Glutamate-Ammonia Ligase / Q000235:genetics* / Q000378:metabolism; D008027:Light; D008969:Molecular Sequence Data; D009584:Nitrogen / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000201:enzymology / Q000235:genetics*; D010946:Plants, Medicinal; D010947:Plants, Toxic; D011401:Promoter Regions, Genetic / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D014026:Nicotiana / Q000201:enzymology / Q000235:genetics*
D011993:Recombinant Fusion Proteins; D005966:Glucuronidase; D005974:Glutamate-Ammonia Ligase; D009584:Nitrogen