Cultivation, characterization and modulation of rat thymic non-lymphoid cells in vitro.
Thymic fragments of young adult rats were cultivated in vitro using an explant technique. Two weeks later, non-lymphoid cells were characterized by enzyme-cytochemistry and immuno-cytochemistry. Based on cytomorphological criteria and cell-specific markers approximately 95% of cells were classified into 3 main types: epithelial cells, macrophages and fibroblasts. The effect of dexamethasone and amphotericin B, known modulators of cell growth in culture was also studied. Quantitative analysis showed that amphotericin B inhibited proliferation of macrophages and epithelial cells, while dexamethasone suppressed proliferation of fibroblasts and promoted growth of epithelial cells.
Colić
M
M
Institute for Experimental Medicine, Military Medical Academy, Belgrade, Yugoslavia.
D000666:Amphotericin B / Q000494:pharmacology; D000818:Animals; D002455:Cell Division; D002478:Cells, Cultured; D003907:Dexamethasone / Q000494:pharmacology; D004847:Epithelial Cells; D005260:Female; D005347:Fibroblasts / Q000166:cytology; D005455:Fluorescent Antibody Technique; D006651:Histocytochemistry; D008264:Macrophages / Q000166:cytology; D008297:Male; D051381:Rats; D013950:Thymus Gland / Q000166:cytology*
D003907:Dexamethasone; D000666:Amphotericin B