Detection and quantification of anti-Ki antibodies by enzyme-linked immunosorbent assay using recombinant Ki antigen.
OBJECTIVE
To establish an enzyme-linked immunosorbent assay (ELISA) for detecting anti-Ki antibody, using a bovine recombinant Ki antigen, and studying its specificity.


METHODS
Sera from 220 patients with various connective tissue diseases were screened, and a prospective study of fluctuations in anti-Ki antibody and clinical course of a woman with systemic lupus erythematosus (SLE) was analyzed, by ELISA:


RESULTS
Anti-Ki antibodies were present in 18.9% of patients with SLE. The titer of anti-Ki antibody in the woman with SLE rose before the onset of pericarditis and pleuritis in this longitudinal study.


CONCLUSIONS
ELISA using a recombinant Ki antigen is useful for the diagnosis of SLE, and it might be useful in estimating disease activity in patients with SLE.
Yamanaka
K
K
Department of Internal Medicine, Juntendo University, School of Medicine, Tokyo, Japan.
Takasaki
Y
Y
Nishida
Y
Y
Shimada
K
K
Shibata
M
M
Hashimoto
H
H
D000818:Animals; D001323:Autoantibodies / Q000032:analysis*; D002417:Cattle; D002846:Chromatography, Affinity; D003240:Connective Tissue Diseases / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000276:immunology; D009687:Nuclear Proteins / Q000737:chemistry / Q000276:immunology*; D010455:Peptides / Q000032:analysis; D018809:Proliferating Cell Nuclear Antigen; D011994:Recombinant Proteins
D001323:Autoantibodies; D009687:Nuclear Proteins; D010455:Peptides; D018809:Proliferating Cell Nuclear Antigen; D011994:Recombinant Proteins