Functional involvement of P-glycoprotein in blood-brain barrier.
P-glycoprotein, an active efflux pump of antitumor agents in multidrug-resistant tumor cells, exists in various normal tissues, including brain capillaries. To study the physiological function of P-glycoprotein expressed in brain capillary endothelium, we established nine mouse brain capillary endothelial cell (MBEC) lines and examined the transport of antitumor agents across the monolayer of MBEC epithelia. In the MBECs, the activities of alkaline phosphatase and gamma-glutamyl transpeptidase, specific markers for brain capillary endothelial cells, were about three times higher than those in other cells including human umbilical vein endothelial cells. By immunoblot analysis, P-glycoprotein was detected in all of the nine MBEC clones. The P-glycoprotein expressed in MBECs specifically bound [125I]iodoaryl azidoprazosin as that in multidrug-resistant cells, and efflux of vincristine was observed in the MBECs. When MBECs were grown on a porous filter membrane, they formed a monolayer of epithelium. By immunoelectron microscopic analysis, P-glycoprotein in MBEC epithelia was shown to be localized to the apical surface of the cells. Moreover, the unidirectional transepithelial transport of vincristine from basal side to apical side was demonstrated in vitro. These observations indicate that P-glycoprotein in brain capillary endothelium prevents vincristine from entering the central nervous system and thus may be one of the functional components of the blood-brain barrier.
Institute of Applied Microbiology, University of Tokyo, Japan.
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T
Tatsuta
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Naito
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T
Oh-hara
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Sugawara
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Tsuruo
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D000970:Antineoplastic Agents / Q000378:metabolism; D001386:Azides / Q000378:metabolism; D001812:Blood-Brain Barrier / Q000502:physiology*; D001921:Brain / Q000098:blood supply / Q000201:enzymology / Q000378:metabolism; D002196:Capillaries / Q000378:metabolism; D002478:Cells, Cultured; D004347:Drug Interactions; D004730:Endothelium, Vascular / Q000201:enzymology / Q000378:metabolism / Q000502:physiology; D006801:Humans; D008562:Membrane Glycoproteins / Q000235:genetics / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D016253:Microscopy, Immunoelectron; D011224:Prazosin / Q000031:analogs & derivatives / Q000378:metabolism; D014407:Tumor Cells, Cultured; D014700:Verapamil / Q000494:pharmacology; D014750:Vincristine / Q000378:metabolism; D005723:gamma-Glutamyltransferase / Q000378:metabolism
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D000970:Antineoplastic Agents; D001386:Azides; D008562:Membrane Glycoproteins; D014750:Vincristine; C041552:azidoprazosin; D014700:Verapamil; D005723:gamma-Glutamyltransferase; D000469:Alkaline Phosphatase; D011224:Prazosin