Molecular analysis of isolates of Streptococcus suis capsular type 2 by restriction-endonuclease-digested DNA separated on SDS-PAGE and by hybridization with an rDNA probe.
This study was undertaken to assess the discriminatory value of restriction endonuclease (RE) digestion patterns of Streptococcus suis chromosomal DNA using polyacrylamide gel electrophoresis (SDS-PAGE) and DNA-rDNA hybridization. For the RE digestion patterns, DNAs were digested separately with the enzymes BamHI and BglII and the resultant fragments were separated by SDS-PAGE. An Escherichia coli rDNA probe derived from pKK3535 was used for the hybridization. Twenty-three S. suis capsular type 2 isolates recovered from diseased and clinically healthy pigs, from a human case, and from a cow were compared in this study. The majority of isolates associated with septicaemia belonged to one restriction endonuclease analysis (REA) profile group. Isolates associated with pneumonia belonged either to the REA profile group of isolates associated with septicaemia or to a second REA profile group. The REA profiles of isolates from clinically healthy animals were more heterogeneous. The REA profile of the type 2 reference strain, S735, which was originally isolated from a pig, was very different from those of the porcine and bovine isolates but similar to the profile of the human isolate. The profiles obtained after rDNA hybridization were more homogeneous. Although different patterns were detected in the 23 isolates, there was no correlation between the source of the isolate and the patterns observed with this technique.
Beaudoin
M
M
Groupe de Recherche sur les Maladies Infectieuses du Porc, Faculté de Médecine Vétérinaire, Université de Montréal, Saint-Hyacinthe, Québec, Canada.
Harel
J
J
Higgins
R
R
Gottschalk
M
M
Frenette
M
M
MacInnes
J I
JI
D000818:Animals; D016667:Bacterial Capsules; D001426:Bacterial Proteins; D002170:Canada; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology; D015342:DNA Probes; D004269:DNA, Bacterial / Q000235:genetics*; D004275:DNA, Ribosomal / Q000235:genetics*; D015245:Deoxyribonuclease BamHI / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics; D005069:Evaluation Studies as Topic; D006801:Humans; D012150:Polymorphism, Restriction Fragment Length; D000091402:Prohibitins; D013290:Streptococcal Infections / Q000382:microbiology / Q000662:veterinary; D017011:Streptococcus suis / Q000145:classification* / Q000235:genetics / Q000472:pathogenicity; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology; D014774:Virulence
D001426:Bacterial Proteins; D015342:DNA Probes; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; C000717331:PHB2 protein, human; D000091402:Prohibitins; D015245:Deoxyribonuclease BamHI; C024404:BglII endonuclease; D015252:Deoxyribonucleases, Type II Site-Specific