Development of a turbidimetric immunoassay for on-line monitoring of proteins in cultivation processes.
An on-line assay for a thermostable pullulanase and antithrombin III (AT III) is described. The assay is based on the formation of aggregates between the protein to be measured and antibodies raised against this protein. Assay automation was achieved by utilizing the flow injection analysis (FIA) principles. The apparatus, a stopped-flow, merging-zone manifold, is described in detail. Since the reaction used in an FIA system does not have to reach equilibrium, it was possible to reduce the time for an assay cycle to 2.5 min. A method for simulating cultivation conditions was developed for assay optimization. Using this method, a detection limit of 1 mg l-1 together with a standard deviation of 1.5 was found. A sandwich ELISA was used as reference assay in the case of AT III and an enzymatic activity assay in the case of pullulanase. Correlation coefficients of 0.988 (AT III) and 0.976 (pullulanase) were determined. The turbidimetric assay was successfully used for pullulanase monitoring during a 240-h cultivation of Clostridium thermosulfurogenes.
Institut für Technische Chemie, Universität Hannover, Germany.
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R
Freitag
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T
Scheper
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K
Schügerl
D000936:Antigen-Antibody Complex / Q000032:analysis*; D000990:Antithrombin III / Q000032:analysis* / Q000096:biosynthesis; D003013:Clostridium / Q000201:enzymology; D004797:Enzyme-Linked Immunosorbent Assay; D006026:Glycoside Hydrolases / Q000032:analysis* / Q000096:biosynthesis; D006801:Humans; D007118:Immunoassay / Q000379:methods; D009391:Nephelometry and Turbidimetry / Q000295:instrumentation / Q000379:methods
D000936:Antigen-Antibody Complex; D000990:Antithrombin III; D006026:Glycoside Hydrolases; C100181:pullulanase