Cloning and mapping of telomere-associated sequences from Hordeum vulgare L.
We present a novel approach to the efficient cloning of telomere-associated sequences and demonstrate its application to the cloning and mapping of these sequences from barley. The method is a modification of the Vectorette PCR technique and allows specific amplification and cloning of subtelomeric sequences. Telomere-associated sequences isolated from barley include hypervariable, repetitive sequences. Polymorphisms detected by subtelomeric markers behaved as Mendelian factors and were mapped to the most distal positions of barley chromosomes 1, 2, and 4.
Kilian
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Department of Crop and Soil Sciences, Washington State University, Pullman 99164-6420.
Kleinhofs
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D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004277:DNA, Single-Stranded; D001467:Hordeum / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D016615:Telomere
D004277:DNA, Single-Stranded