Evidence that multiple defects in cell-surface molecule interactions across species differences are responsible for diminished xenogeneic T cell responses.
The purpose of the present study was to identify which of the several possible defects in cell-surface-molecule interactions are responsible for diminished mouse helper T cell responses to xenoantigens. We measured primary mouse anti-monkey, anti-pig, and anti-human proliferation in vitro in experimental systems in which potential defects were partially corrected by lymphokine supplementation and/or the use of transgenic or hybridoma cell populations. We found that the diminished mouse helper T cell responses to xenoantigens result from at least two defects in cell-surface-molecule interactions between T cells and xenogeneic APCs, specifically TCR and/or CD8 interactions with xenogeneic class I MHC molecules and accessory molecule interactions with their ligands (probably LFA-1 with ICAM-1/ICAM-2 and/or LFA-2 with LFA-3). Other investigators have identified additional defects, such as in lymphokine function across species differences. Thus, there appear to be multiple defects responsible for the diminished cellular immune response to xenoantigens.
Department of Surgery, Massachusetts General Hospital, Boston 02114.
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RD
Moses
H J
HJ
Winn
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Auchincloss
D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology; D000945:Antigens, Differentiation, T-Lymphocyte / Q000502:physiology; D015478:Antigens, Heterophile / Q000276:immunology*; D018801:CD2 Antigens; D015704:CD4 Antigens / Q000502:physiology; D016827:CD8 Antigens / Q000502:physiology; D002478:Cells, Cultured; D005260:Female; D015395:Histocompatibility Antigens Class I / Q000032:analysis; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D006801:Humans; D008213:Lymphocyte Activation; D008222:Lymphokines / Q000494:pharmacology; D008252:Macaca fascicularis; D008297:Male; D051379:Mice; D011971:Receptors, Immunologic / Q000502:physiology; D011994:Recombinant Proteins / Q000494:pharmacology; D013045:Species Specificity; D013552:Swine; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*
D000945:Antigens, Differentiation, T-Lymphocyte; D015478:Antigens, Heterophile; D018801:CD2 Antigens; D015704:CD4 Antigens; D016827:CD8 Antigens; D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II; D008222:Lymphokines; D011971:Receptors, Immunologic; D011994:Recombinant Proteins