Some technical parameters influencing the precision and accuracy of fragment size determination for RFLP's.
A comparison was carried out between 3 computer-assisted systems for the estimation of DNA fragment length: (1) the "Digitab" system (developed at our institute), (2) the FBI analysis system (FBI Quantico, USA) and (3) the BioImage system (Waters/Millipore, USA). Both the FBI system and the BioImage system were found to be much more precise than the manual Digitab system. In an additional experiment, a possible influence of the field strength on the "accuracy" of fragment size measurement was checked. Lowering the field strength to approx. 1V/cm led to a statistically significant increase in the measured fragment size.
Institut für Rechtsmedizin, Universität Münster, Bundesrepublik Deutschland.
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Puers
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Wiegand
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Brinkmann
D003199:Computer Systems; D006801:Humans; D012150:Polymorphism, Restriction Fragment Length; D011786:Quality Control; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*; D015203:Reproducibility of Results; D012984:Software