Discordant segregation of Na+,K(+)-adenosine triphosphatase alleles and essential hypertension.
OBJECTIVE
To determine whether the alpha 2 and or beta 1 isoforms of the Na+,K(+)-adenosine triphosphatase (Na+,K(+)-ATPase) are involved in the pathogenesis of essential hypertension.


METHODS
Segregation analysis of polymorphic DNA markers was used to test the involvement of Na+,K(+)-ATPase in essential hypertension.


METHODS
Children with persistent hypertension having one parent with essential hypertension were included in the study. Criteria for persistent hypertension were blood pressure readings with systolic and/or diastolic levels exceeding the 95th percentile based upon age and sex. The diagnosis of hypertension for adults, including parents and older siblings, was confirmed using criteria recommended in the 1988 report of the Joint National Committee on Detection, Evaluation, and Treatment of High Blood Pressure.


RESULTS
In three essential hypertensive families consisting of 18 members including 11 hypertensives, several obligate recombinants between the Na+,K(+)-ATPase alpha 2 isoform marker and the hypertension phenotype were observed. Similarly, in one hypertension family consisting of four members, obligate recombinants between the beta 1 isoform marker and the disease were observed.


CONCLUSIONS
The discordant segregation of the alpha 2 and beta 1 isoform markers and essential hypertension suggests that neither the Na+,K(+)-ATPase alpha 2 nor beta 1 isoform genes play a primary role in the pathogenesis of hypertension in the families studied.
Department of Molecular Genetics, Biochemistry and Microbiology, University of Cincinnati College of Medicine, OH.
M M
MM
Shull
D
D
Hassenbein
J
J
Loggie
S
S
Daniels
A
A
King
T
T
Burton
J B
JB
Lingrel
D000328:Adult; D002648:Child; D004247:DNA / Q000032:analysis*; D005190:Family; D005816:Genetic Complementation Test; D008040:Genetic Linkage; D006801:Humans; D006973:Hypertension / Q000235:genetics*; D007527:Isoenzymes / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D010375:Pedigree; D012150:Polymorphism, Restriction Fragment Length; D000254:Sodium-Potassium-Exchanging ATPase / Q000235:genetics*
D007527:Isoenzymes; D004247:DNA; D000254:Sodium-Potassium-Exchanging ATPase