Monoclonal antibody to Japanese encephalitis virus cross-reacting with histones present in the cell nuclei.
An immunoglobulin G (IgG2b) class of monoclonal antibody (MoAb, NHA-1) raised against Japanese encephalitis virus (JEV) E glycoprotein, reacted with the viral antigen expressed in cytoplasm of the infected cells and also with the cell nuclei, by an indirect fluorescent antibody technique (FA). The NHA-1 reactivity to nuclei was found to be due to its recognizing a JEV cross-reactive epitope present on the nuclear histones. Adsorption with calf thymus histones (type II-AS) showed a drop in NHA-1 reactivity to both JEV and histones by an enzyme-linked immunosorbent assay (ELISA) and indirect FA; the drop was higher against the histones. The MoAb recognized specifically the viral antigens expressed on the infected porcine kidney cell surface by a modified indirect FA. ELISA carried out with glutaraldehyde-fixed antigens showed an almost 2-fold increase in the reactivity over unfixed JEV antigen but none for the histones. Thus, the results indicate that histones share a sequential homology with E glycoprotein of JEV, which might lead to an autoimmune disorder induced due to the molecular mimicry between these two antigens.
National Institute of Virology, Pune, India.
A K
AK
Gupta
S
S
Bhattacharya
V J
VJ
Lad
S A
SA
Sarthi
M
M
Kutubuddin
S N
SN
Ghosh
K
K
Banerjee
D000818:Animals; D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D002467:Cell Nucleus / Q000276:immunology; D002478:Cells, Cultured; D003429:Cross Reactions; D004664:Encephalitis Virus, Japanese / Q000276:immunology*; D006657:Histones / Q000276:immunology*; D007074:Immunoglobulin G; D013552:Swine; D014709:Vero Cells; D015678:Viral Structural Proteins / Q000276:immunology
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D006657:Histones; D007074:Immunoglobulin G; D015678:Viral Structural Proteins