Two intragenic polymorphisms of the APC-gene detected by PCR and enzymatic digestion.
The gene causing adenomatous polyposis coli (APC) has recently been cloned. Three intragenic polymorphisms were reported to be detectable by single-strand conformation polymorphism analysis. Here, we describe an assay using polymerase-chain-reaction-based amplification and subsequent enzymatic digestion of genomic sequences to identify polymorphic sites at nucleotide positions 1458 and 5037 of the APC gene.
Institut für Humangenetik der Universität, Erlangen, Federal Republic of Germany.
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C
Kraus
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WG
Ballhausen
D011125:Adenomatous Polyposis Coli / Q000235:genetics*; D001483:Base Sequence; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length