Basic fibroblast growth factor induces proliferation of a rat pancreatic cancer cell line. Inhibition by somatostatin.
AR4-2J, a rat pancreatic acinar-tumor cell line, was used to investigate long-term effects of basic fibroblast growth factor (bFGF) and somatostatin on pancreatic cancer cells. We observed that bFGF stimulated cell proliferation when cells were cultured in serum-free medium. The effect was dose-dependent with half-maximal and maximal effects at 25 pM and I nM bFGF, respectively. The somatostatin analog SMS 201-995 (SMS) decreased the growth-promoting effect of bFGF. The maximal effect was observed at I nM SMS and the half-maximal effect at 20 pM SMS. Characterization of bFGF receptor-binding properties with [125I]bFGF revealed that AR4-2J cells exhibited 2 classes of bFGF binding site with respective KD values of 47 pM and 3 nM and binding capacities of 14 fmol and 0.9 pmol/10(6) cells. High-affinity receptors correlated with bFGF stimulation of AR4-2J cell growth, suggesting that the effects of bFGF are receptor-mediated.
INSERM U 151, CHU Rangueil, Toulouse, France.
M
M
Bensaïd
N
N
Tahiri-Jouti
C
C
Cambillau
N
N
Viguerie
B
B
Colas
C
C
Vidal
J P
JP
Tauber
J P
JP
Estève
C
C
Susini
N
N
Vaysse
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D016222:Fibroblast Growth Factor 2 / Q000037:antagonists & inhibitors / Q000378:metabolism / Q000494:pharmacology*; D010190:Pancreatic Neoplasms / Q000473:pathology*; D051381:Rats; D011956:Receptors, Cell Surface / Q000032:analysis; D017468:Receptors, Fibroblast Growth Factor; D013004:Somatostatin / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D011956:Receptors, Cell Surface; D017468:Receptors, Fibroblast Growth Factor; D016222:Fibroblast Growth Factor 2; D013004:Somatostatin