Production and characterization of a polyclonal antibody to the c-erbB-3 protein: examination of c-erbB-3 protein expression in adenocarcinomas.
A polyclonal rabbit antibody was raised to the c-erbB-3 protein using a synthetic peptide corresponding to amino acids 1229-1241 of the predicted protein sequence of c-erbB-3. In Western blot analysis this antibody detects a single band at approximately 165 kD in a c-erbB-3 transfected (293/HER-3) human cell line. c-erbB-3 protein expression was then examined in a variety of adenocarcinomas. Expression of c-erbB-3 protein was indicated by membrane and/or cytoplasmic tumour cell immunoreactivity in formalin-fixed, paraffin-embedded tissue sections. c-erbB-3 protein was detected in a series of 13 out of 14 primary breast carcinomas, 3 of 5 gastric adenocarcinomas, 8 of 9 colonic adenocarcinomas, 2 of 9 prostatic adenocarcinomas, 0 of 6 renal cell carcinomas, 1 of 4 primary lung adenocarcinomas, and 5 of 7 endometrial adenocarcinomas. Immunohistochemical expression of the c-erbB-3 protein appears to be a relatively common event in adenocarcinomas, and further studies are now warranted to establish the role of the c-erbB-3 protein in neoplasia.
University Department of Histopathology, Queen's Medical Centre, Nottingham, U.K.
D N
DN
Poller
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I
Spendlove
C
C
Baker
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R
Church
I O
IO
Ellis
G D
GD
Plowman
R J
RJ
Mayer
D000230:Adenocarcinoma / Q000737:chemistry*; D000818:Animals; D000906:Antibodies / Q000302:isolation & purification*; D015153:Blotting, Western; D001943:Breast Neoplasms / Q000737:chemistry; D002292:Carcinoma, Renal Cell / Q000737:chemistry; D002462:Cell Membrane / Q000737:chemistry; D003110:Colonic Neoplasms / Q000737:chemistry; D003593:Cytoplasm / Q000737:chemistry; D016889:Endometrial Neoplasms / Q000737:chemistry; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007680:Kidney Neoplasms / Q000737:chemistry; D008175:Lung Neoplasms / Q000737:chemistry; D008297:Male; D018739:Oncogene Proteins v-erbB; D011471:Prostatic Neoplasms / Q000737:chemistry; D011518:Proto-Oncogene Proteins / Q000032:analysis / Q000276:immunology*; D011817:Rabbits; D020893:Receptor, ErbB-3; D015682:Retroviridae Proteins, Oncogenic / Q000276:immunology* / Q000378:metabolism; D013274:Stomach Neoplasms / Q000737:chemistry
D000906:Antibodies; D018739:Oncogene Proteins v-erbB; D011518:Proto-Oncogene Proteins; D015682:Retroviridae Proteins, Oncogenic; D020893:Receptor, ErbB-3