Production of androsta-1,4-diene-3,17-dione from cholesterol using immobilized growing cells of Mycobacterium sp. NRRL B-3683 adsorbed on solid carriers.
Living cells of Mycobacterium sp. NRRL B-3683 were immobilized by adsorption on different types of solid carriers in order to produce androsta-1,4-diene-3,17-dione (ADD) from cholesterol. Activated alumina proved to be the most preferred carrier for long-term operation when glucose and peptone were added to the reaction medium. In a repeated-batch process, the maximum productivity of ADD was about 0.19 g/l per day with a molar conversion rate of 77% when 1.0 g/l of cholesterol was added to the reaction medium. The half-life of the immobilized cells was more than 45 days and the system could be reactivated by incubating the immobilized cells in a cell growth medium.
Graduate Institute of Agricultural Chemistry, National Taiwan University, Taipei, Republic of China.
C Y
CY
Lee
W H
WH
Liu
D000327:Adsorption; D000537:Aluminum Oxide; D000730:Androstadienes / Q000378:metabolism* / Q000494:pharmacology; D002784:Cholesterol / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D007218:Industrial Microbiology / Q000379:methods*; D009161:Mycobacterium / Q000187:drug effects / Q000378:metabolism*
D000730:Androstadienes; C006573:1,4-androstadiene-3,17-dione; D002784:Cholesterol; D005947:Glucose; D000537:Aluminum Oxide