An aqueous hydroxypropylstarch-poly(ethylene glycol) two-phase system for extraction of alpha-lactalbumin and beta-lactoglobulin from bovine whey.
The partitioning of alpha-lactalbumin and beta-lactoglobulin from bovine whey has been studied in an aqueous poly(ethylene glycol) (PEG)-hydroxypropylstarch two-phase system. The influence of several parameters including concentrations of polymers, sodium phosphate buffer, KSCN, and of PEG palmitate, with and without the presence of Ca2+, on the partitioning of the proteins has been investigated. The separation of the two proteins was demonstrated by counter-current distribution. A purification procedure for both proteins has been developed by using PEG-hydroxypropylstarch two-phase system. This system is compared with the more costly standard system based on PEG and dextran. The possible use of the aqueous two-phase systems for batch extraction for large scale purification of these whey proteins is discussed.
Ortín
A
A
Department of Biochemistry, University of Lund, Sweden.
Cebrian
J A
JA
López-Pérez
M J
MJ
Johansson
G
G
D000818:Animals; D002417:Cattle; D007768:Lactalbumin / Q000302:isolation & purification*; D007782:Lactoglobulins / Q000302:isolation & purification*; D008892:Milk / Q000032:analysis; D011092:Polyethylene Glycols; D012995:Solubility; D013213:Starch
D007782:Lactoglobulins; D011092:Polyethylene Glycols; D013213:Starch; D007768:Lactalbumin