Further data on the development of SRIF-like immunoreactive nerve cell populations in the chick embryo brain stem. II. Midbrain tegmentum.
Further immunocytochemical analysis of the neuroblasts with SRIF-like immunoreactivity (ir) was carried out on the chick embryo midbrain tegmentum. 5 or 100 microns mesencephalon sections were obtained from 60 White Leghorn chick embryos at stages (E = Embryonic days) ranging from E4 1/2 to E18 and incubated with rabbit polyclonal antibodies against synthetic cyclic Somatostatin-14, according to PAP-DAB technique. In the midbrain tegmentum the ir appeared as from E12. From E12 to E13 1/2-E14 the ir distribution gradually changed. From E14 to E18 numbers and spatial arrangement of the positive neuroblast groups did not show substantial changes; in these respects the ir distributional pattern proved to be similar to the one observed by the Authors in adult animals. From E17 to E18 a decrease in the positive neuroblast density appeared to occur, particularly in a ventrally placed group. These results are consistent with a possible local regulative role of the SRIF.
Institute of Human Anatomy, University of Bari.
G
G
Nicolardi
A
A
Rizzi
V
V
Benagiano
R
R
Arena
F
F
Fanelli
M E
ME
Camosso
G
G
Ambrosi
D000818:Animals; D002642:Chick Embryo / Q000737:chemistry*; D007124:Immunoenzyme Techniques; D009024:Morphogenesis; D009474:Neurons / Q000737:chemistry*; D013004:Somatostatin / Q000032:analysis*; D013681:Tegmentum Mesencephali / Q000737:chemistry* / Q000196:embryology*
D013004:Somatostatin