Immunologically active metallic ion-containing polysaccharides of Achyrocline satureioides.
Two homogeneous, metallic ion-containing pectic polysaccharides with mean M(r)s of 7600 and 15,000 were isolated from dried aerial parts of Achyrocline satureioides by anion exchange column chromatography on DEAE-Sepharose CL-6B and gel filtration column chromatography on Fractogel TSK HW-50 (S). The structures, as determined by methylation analysis, carboxyl reduction, and partial acid hydrolysis, were shown to be rhamnogalacturonans. Both pectins show a pronounced anticomplementary effect in vitro. The larger carbohydrate AS 4 of higher M(r) exerts anti-inflammatory activity and a strong enhancement of phagocytosis in vivo.
Complex Carbohydrate Research Center, Athens, GA 30602.
J
J
Puhlmann
U
U
Knaus
L
L
Tubaro
W
W
Schaefer
H
H
Wagner
D002240:Carbohydrate Sequence; D003169:Complement Inactivator Proteins / Q000737:chemistry / Q000302:isolation & purification; D008670:Metals / Q000737:chemistry; D008969:Molecular Sequence Data; D010368:Pectins / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*; D010946:Plants, Medicinal / Q000737:chemistry* / Q000276:immunology
D003169:Complement Inactivator Proteins; D008670:Metals; C076293:rhamnogalacturonan AS4; C076292:rhamnogalacturonan AS3; D010368:Pectins