Characterization of the cytokeratins of human colonic, pancreatic, and gastric adenocarcinoma cell lines.
Cytokeratin-type intermediate filaments are a polygenic family of insoluble proteins that vary according to cell of origin and have been proposed as potentially useful markers of differentiation in epithelial malignancies. Because gastrointestinal malignancies resemble each other in their expression of many soluble antigens, we compared the cytokeratins of seven colonic, three gastric, six pancreatic, and one duodenal carcinoma cell lines, and one colon villous adenoma cell line. Cytokeratins were characterized by one- and two-dimensional gel electrophoresis, immunoblotting, and immunocytochemistry. These cell lines expressed combinations of cytokeratins 7, 8, 18, and 19, which are typical of the "simple" epithelial pattern found in normal ductal and glandular tissues of the gastrointestinal tract. However, pancreatic carcinoma cell lines expressed additional cytokeratins that are normally found in stratified squamous epithelium and epidermoid (squamous cell) carcinomas. These additional cytokeratins consisted of cytokeratin 16 in all six cell lines and cytokeratins 4, 13, and 16 in one cell line. These results suggest that cytokeratin patterns represent stable markers that may aid in distinguishing gastrointestinal malignancies.
Gastrointestinal Research Laboratory, Veterans Administration Medical Center, San Francisco, California 94121.
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P
Rafiee
S B
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Ho
R S
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Bresalier
E J
EJ
Bloom
J H
JH
Kim
Y S
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Kim
D000230:Adenocarcinoma / Q000737:chemistry*; D000818:Animals; D002460:Cell Line; D003110:Colonic Neoplasms / Q000737:chemistry*; D003937:Diagnosis, Differential; D006801:Humans; D007633:Keratins / Q000302:isolation & purification*; D051379:Mice; D010190:Pancreatic Neoplasms / Q000737:chemistry*; D013274:Stomach Neoplasms / Q000737:chemistry*; D014407:Tumor Cells, Cultured
D007633:Keratins