Comparison of growth kinetics of producing and nonproducing hybridoma cells in batch culture.
Several clones of nonproducing cells were isolated from a continuous culture of hybridoma cells, which were originally producing antibody. Their behavior was compared to that of the producing cells in batch culture. The growth kinetics of five out of six clones exhibited higher specific growth rate, higher yield of cell mass on glutamine, and lower yields of lactate and ammonium. The implications of the comparisons for growth of hybridoma cultures are discussed.
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Department of Chemical Engineering and Materials Science, University of Minnesota, Minneapolis 55455.
Hu
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D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D002455:Cell Division; D002470:Cell Survival; D002999:Clone Cells; D046508:Culture Techniques / Q000379:methods; D006801:Humans; D006825:Hybridomas / Q000166:cytology* / Q000276:immunology; D007074:Immunoglobulin G / Q000096:biosynthesis; D007700:Kinetics; D000509:alpha-Fetoproteins / Q000276:immunology
D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D000509:alpha-Fetoproteins