Inactivation of proteins and other biological macromolecules during chromatographic methods of bioseparation.
The denaturation of proteins and other biological macromolecules such as gentamycin, mRNAs, and long-chain fatty acids during their separation by different chromatographic techniques is analyzed. Non-conventional techniques such as centrifugal partition chromatography are also examined. Particular attention is paid to the denaturing mechanisms prevalent under processing conditions, and how denaturation may perhaps be alleviated under laboratory conditions or during scale-up. The available mechanistic studies shed physical insights into the conformational behavior of proteins on chromatographic columns. Mechanistic studies of other biological macromolecule separation on columns is rare. Numbers for both recovery and purity of the biological product are presented wherever available. Scale-up studies are rare, nevertheless, those that are presented together do provide significant and valuable information, and may be generalized to other systems with caution.
Chemical Engineering Department, University of Mississippi, University 38677-9740.
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Sadana
D000818:Animals; D001709:Biotechnology; D002845:Chromatography / Q000379:methods*; D002846:Chromatography, Affinity / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002852:Chromatography, Ion Exchange / Q000379:methods; D006801:Humans; D046911:Macromolecular Substances; D008956:Models, Chemical; D011489:Protein Denaturation; D011506:Proteins / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000302:isolation & purification*
D046911:Macromolecular Substances; D011506:Proteins