Substrate specificity of alpha-galactosidase from Aspergillus niger 5-16.
This paper describes the specificity of Aspergillus niger 5-16 alpha-galactosidase toward various oligosaccharides having terminal galactose or stub galactose or both on the oligosaccharide. The galactosidase rapidly hydrolyzed p-nitrophenyl-alpha-D-galactopyranoside, but hardly liberated galactose from melibiose, manninotriose, 6(3)-alpha-D-galactosylmannotriose, etc. On the other hand, the enzyme tore off the stub galactoses attached to the inner mannoses of the main-chain of galactomannooligosaccharides, but not the terminal galactoses attached to the non-reducing-end mannoses of the main-chain. Thus, the substrate specificity of A. niger 5-16 alpha-galactosidase is quite different from that of Mortierella vinacea alpha-galactosidase.
Institute of Applied Biochemistry, University of Tsukuba, Ibaraki, Japan.
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R
Kaneko
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Kusakabe
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Ida
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K
Murakami
D001234:Aspergillus niger / Q000201:enzymology*; D002240:Carbohydrate Sequence; D002846:Chromatography, Affinity; D002855:Chromatography, Thin Layer; D008361:Mannosidases / Q000302:isolation & purification; D008553:Melibiose / Q000378:metabolism; D008969:Molecular Sequence Data; D009090:Mucorales / Q000201:enzymology*; D009598:Nitrophenylgalactosides / Q000378:metabolism; D009844:Oligosaccharides / Q000378:metabolism*; D013379:Substrate Specificity; D014312:Trisaccharides / Q000378:metabolism; D000519:alpha-Galactosidase / Q000378:metabolism*; D044902:beta-Mannosidase
D009844:Oligosaccharides; D014312:Trisaccharides; C069155:manninotriose; D009598:Nitrophenylgalactosides; C059200:4-nitrophenylgalactoside; D008553:Melibiose; D008361:Mannosidases; D000519:alpha-Galactosidase; D044902:beta-Mannosidase