Glutamate receptors of Drosophila melanogaster: cloning of a kainate-selective subunit expressed in the central nervous system.
We report the isolation and functional characterization of cDNAs encoding a Drosophila kainate-selective glutamate receptor. The deduced mature 964-residue protein (DGluR-I) is 108,482 Da and exhibits significant homology to mammalian glutamate receptor subunits. Injection of DGluR-I cRNA into Xenopus oocytes generated kainate-operated ion channels which were blocked by the selective non-N-methyl-D-aspartate receptor antagonist 6-cyano-7-nitro-quinoxaline-2,3-dione and philanthotoxin. DGluR-I transcripts are differentially expressed during Drosophila development and, in late embryogenesis, accumulate in the central nervous system.
Abteilung Neurochemie, Max-Planck-Institut für Hirnforschung, Frankfurt/Main, Federal Repoublic of Germany.
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A
Ultsch
C M
CM
Schuster
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B
Laube
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P
Schloss
B
B
Schmitt
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H
Betz
D018750:6-Cyano-7-nitroquinoxaline-2,3-dione; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D004331:Drosophila melanogaster / Q000235:genetics* / Q000378:metabolism; D007608:Kainic Acid / Q000378:metabolism / Q000494:pharmacology*; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D009420:Nervous System / Q000378:metabolism; D009838:Oligodeoxyribonucleotides; D009865:Oocytes / Q000187:drug effects / Q000502:physiology; D010636:Phenols / Q000494:pharmacology; D011073:Polyamines / Q000494:pharmacology; D016133:Polymerase Chain Reaction / Q000379:methods; D011810:Quinoxalines / Q000494:pharmacology; D017470:Receptors, Glutamate / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic; D014862:Wasp Venoms / Q000494:pharmacology; D014981:Xenopus
D046911:Macromolecular Substances; D009838:Oligodeoxyribonucleotides; D010636:Phenols; D011073:Polyamines; D011810:Quinoxalines; D017470:Receptors, Glutamate; D014862:Wasp Venoms; C074003:philanthotoxin 343; D018750:6-Cyano-7-nitroquinoxaline-2,3-dione; D004247:DNA; D007608:Kainic Acid