Examples of expression systems based on animal RNA viruses: alphaviruses and influenza virus.
Successful recovery of RNA viruses and functional RNA replicons from cDNA has greatly facilitated molecular genetic analyses of viral proteins and cis-regulatory elements. This technology allows the use of RNA virus replication machinery to express heterologous sequences. Both positive-strand and negative-strand animal RNA viruses have been engineered to produce chimeric viruses expressing protective epitopes from other pathogens and for transient expression of heterologous sequences.
Department of Molecular Microbiology, Washington University School of Medicine, St Louis, Missouri 63110-1093.
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D000524:Alphavirus / Q000235:genetics; D001709:Biotechnology; D015870:Gene Expression; D005818:Genetic Engineering; D009975:Orthomyxoviridae / Q000235:genetics; D012328:RNA Viruses / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics
D012367:RNA, Viral