Cytoskeleton and molecular mechanisms in neurotransmitter release by neurosecretory cells.
The process of exocytosis is a fascinating interplay between secretory vesicles and cellular components. Secretory vesicles are true organelles which not only store and protect neurotransmitters from inactivation but also provide the cell with efficient carriers of material for export. Different types of secretory vesicles are described and their membrane components compared. Associations of several cytoplasmic proteins and cytoskeletal components with secretory vesicles and the importance of such associations in the mechanism of secretion are discussed. A description of possible sites of action for Ca2+ as well as possible roles for calmodulin, G-proteins and protein kinase C in secretion are also presented. Important aspects of the cytoskeleton of neurosecretory cells are discussed. The cytoskeleton undergoes dynamic changes as a result of cell stimulation. These changes (i.e. actin filament disassembly) which are a prelude to exocytosis, play a central role in secretion. Moreover, advanced electrophysiological techniques which allow the study of secretory vesicle-plasma membrane fusion in real-time resolution and at the level of the single secretory vesicle, have also provided a better understanding of the secretory process.
Department of Pharmacology, University of Ottawa, Ontario, Canada.
J M
JM
Trifaró
M L
ML
Vitale
A
A
Rodríguez Del Castillo
D002147:Calmodulin / Q000037:antagonists & inhibitors / Q000378:metabolism; D002352:Carrier Proteins; D003599:Cytoskeleton / Q000502:physiology*; D009490:Neurosecretory Systems / Q000502:physiology*; D018377:Neurotransmitter Agents / Q000378:metabolism*
D002147:Calmodulin; D002352:Carrier Proteins; D018377:Neurotransmitter Agents