Routes to active proteins from transformed microorganisms.
Over-expression of recombinant proteins in microbial hosts results in the formation of active soluble protein or of insoluble aggregates (inclusion bodies). Efficient in vitro refolding strategies have been developed to reactivate inactive proteins from inclusion bodies. Co-expression of molecular chaperones may provide a tool to promote correct structure formation of recombinant proteins in vivo.
Buchner
J
J
Institut für Biophysik and Biochemie, Universität Regensburg, Germany.
Rudolph
R
R
D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D006801:Humans; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D014169:Transformation, Bacterial
D011994:Recombinant Proteins