An easy-to-handle semi-automated method for media development using a colorimetric viability assay and fractional factorial designs.
A rapid and effective semi-automated screening method has been developed for the development of growth media for mammalian cell culture. The method has proven to be a powerful tool for preliminary evaluation and comparison of new media formulations, but has some inherent disadvantages, which are important to recognise in order to interpret the results.
Department of Biotechnology, Technical University of Denmark, Lyngby.
P
P
Rexen
J V
JV
Kierulff
C
C
Emborg
D000818:Animals; D001331:Automation; D016466:CHO Cells; D002452:Cell Count; D002470:Cell Survival; D002478:Cells, Cultured; D003124:Colorimetry; D006224:Cricetinae; D003470:Culture Media; D006863:Hydrogen-Ion Concentration; D051379:Mice; D013778:Tetrazolium Salts; D013844:Thiazoles
D003470:Culture Media; D013778:Tetrazolium Salts; D013844:Thiazoles; C022616:thiazolyl blue