Characterization of radiation/fusion hybrids containing parts of human chromosome 10 and their use in mapping chromosome 10-specific probes.
We have characterized a panel of somatic cell hybrid cell lines which contain different portions of human chromosome 10. Genomic DNA from the somatic cell hybrids was tested for hybridization with each of an ordered set of probes used previously to construct a genetic map of chromosome 10, as well as several additional probes, previously localized by in situ hybridization. Hybridization of an unmapped probe to the cell line DNAs can be used to determine its most likely position on the chromosome relative to the mapped set of probes. Genomic DNA from two of the cell lines has been used to construct region-specific cosmid and bacteriophage libraries, and clones derived from these libraries were localized by hybridization to the panel of hybrid cell lines. Several of these probes reveal restriction fragment length polymorphisms which have been genetically mapped. Three of the probes map near the locus for multiple endocrine neoplasia type 2A, and one of these probes, BG-JC353 (D10S167), maps between RBP3 and TB14.34 (D10S34). Another probe, CRI-J282 (D10S104), is close to the FNRB locus. The panel of hybrid cell lines is thus useful for rapidly localizing unmapped probes and as a source of DNA for the construction of recombinant libraries derived from specific regions of the chromosome.
Rothschild
C B
CB
Department of Biochemistry, Bowman Gray School of Medicine, Wake Forest University, Winston-Salem, North Carolina 27157.
Noll
W W
WW
Gravius
T C
TC
Schuster
M K
MK
Nutile-McMenemy
N
N
Jones
C
C
Bowden
D W
DW
D002874:Chromosome Mapping; D002879:Chromosomes, Human, Pair 10; D015342:DNA Probes / Q000235:genetics; D004706:Endodeoxyribonucleases / Q000378:metabolism; D008040:Genetic Linkage / Q000235:genetics; D005819:Genetic Markers / Q000235:genetics*; D006801:Humans; D006822:Hybrid Cells; D009693:Nucleic Acid Hybridization; D012150:Polymorphism, Restriction Fragment Length
D015342:DNA Probes; D005819:Genetic Markers; D004706:Endodeoxyribonucleases