Enzyme sensor-FIA-system for on-line monitoring of glucose, lactate and glutamine in animal cell cultures.
Enzyme sensors for glucose, lactate and glutamine were connected via flow-injection analysis (FIA) devices to two different bioprocesses. They were used for on-line process control of perfused bioreactor systems containing mammalian cell lines producing a monoclonal antibody and recombinant interleukin-2. The biosensor system gives direct access to important process data which can be used as control parameters for long term cell cultivation systems.
Zentralinstitut für Molekularbiologie, Berlin-Buch, F.R.G.
R
R
Renneberg
G
G
Trott-Kriegeskorte
M
M
Lietz
V
V
Jäger
M
M
Pawlowa
G
G
Kaiser
U
U
Wollenberger
F
F
Schubert
R
R
Wagner
R D
RD
Schmid
D000818:Animals; D015374:Biosensing Techniques; D002138:Calibration; D002478:Cells, Cultured; D005947:Glucose / Q000032:analysis*; D005973:Glutamine / Q000032:analysis*; D007376:Interleukin-2 / Q000096:biosynthesis; D007773:Lactates / Q000032:analysis*; D019344:Lactic Acid; D051379:Mice; D009862:Online Systems; D051381:Rats
D007376:Interleukin-2; D007773:Lactates; D005973:Glutamine; D019344:Lactic Acid; D005947:Glucose