B cell presentation of a tolerogenic signal to Th clones.
Lightly irradiated (950 R) splenic B cells were inefficient, in comparison to unseparated spleen cells, in stimulating antigen-specific proliferation of Th1 clones specific for human gamma globulin (HGG). This inefficiency was due to antigen-specific inactivation: Th1 clones preincubated with HGG and lightly irradiated B cells or mitomycin C-treated B cells were unable to proliferate to HGG in secondary cultures. In contrast to Th1 clones, Th2 clones proliferated well in response to B cell APC, and showed no decrease in their subsequent antigen-induced proliferative capacity after exposure to lightly irradiated B cells and HGG. However, preincubation of Th2 with lightly irradiated B cells and HGG did inactivate the capacity of Th2 to provide help for antibody production in secondary cultures. These results suggest that under certain conditions B cells may present antigen to Th1 and Th2 cells in a tolerogenic rather than an immunogenic manner.
Department of Immunology, Research Institute of Scripps Clinic, La Jolla, California 92037.
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Gilbert
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Weigle
D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology* / Q000528:radiation effects; D002478:Cells, Cultured; D005404:Fixatives; D005557:Formaldehyde / Q000737:chemistry; D007108:Immune Tolerance; D066298:In Vitro Techniques; D008213:Lymphocyte Activation; D008211:Lymphocyte Cooperation; D008297:Male; D051379:Mice; D008805:Mice, Inbred A; D011108:Polymers / Q000737:chemistry; D051381:Rats; D011917:Rats, Inbred Lew; D013154:Spleen / Q000166:cytology; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*; D005719:gamma-Globulins / Q000276:immunology
D005404:Fixatives; D011108:Polymers; D005719:gamma-Globulins; D005557:Formaldehyde; C003043:paraform