Examples of expression systems based on animal RNA viruses: alphaviruses and influenza virus.
Successful recovery of RNA viruses and functional RNA replicons from cDNA has greatly facilitated molecular genetic analyses of viral proteins and cis-regulatory elements. This technology allows the use of RNA virus replication machinery to express heterologous sequences. Both positive-strand and negative-strand animal RNA viruses have been engineered to produce chimeric viruses expressing protective epitopes from other pathogens and for transient expression of heterologous sequences.
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Department of Molecular Microbiology, Washington University School of Medicine, St Louis, Missouri 63110-1093.
D000524:Alphavirus / Q000235:genetics; D001709:Biotechnology; D015870:Gene Expression; D005818:Genetic Engineering; D009975:Orthomyxoviridae / Q000235:genetics; D012328:RNA Viruses / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics
D012367:RNA, Viral