Interleukin-11 enhances human megakaryocytopoiesis in vitro.
We investigated the effect of recombinant human interleukin-11 (rhIL-11) on human megakaryocytopoiesis. Nonadherent and T-cell-depleted human bone marrow (BM) mononuclear cells were cultured in a serum-free agar culture system. rhIL-11 alone did not stimulate the growth of human megakaryocyte colonies. However, when rhIL-11 was combined with optimal or suboptimal doses of rhIL-3, the number and size of the megakaryocyte colonies increased. The same results were obtained when highly purified BM CD34-positive cells were used as target cells. Next, we investigated the effect of rhIL-11 on the ploidy of megakaryocytes. The ploidy distribution of individual cells in megakaryocyte colonies obtained by rhIL-11 in combination with rhIL-3 was significantly shifted towards higher values. Furthermore, when highly purified CD41-positive BM cells were cultured in the presence of rhIL-11, the ploidy distribution was shifted towards higher values. This effect was not suppressed by anti-IL-6 antibody. These results suggest that rhIL-11 acts directly as a megakaryocyte potentiator and may play a role in regulating human megakaryocytopoiesis.
Teramura
M
M
Department of Medicine, Tokyo Women's Medical College, Japan.
Kobayashi
S
S
Hoshino
S
S
Oshimi
K
K
Mizoguchi
H
H
D015703:Antigens, CD / Q000032:analysis; D018952:Antigens, CD34; D001854:Bone Marrow Cells; D002469:Cell Separation; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D006410:Hematopoiesis; D006412:Hematopoietic Stem Cells / Q000166:cytology; D006801:Humans; D017370:Interleukin-11; D007377:Interleukin-3 / Q000494:pharmacology; D007378:Interleukins / Q000494:pharmacology*; D008533:Megakaryocytes / Q000166:cytology* / Q000276:immunology; D011003:Ploidies; D011994:Recombinant Proteins / Q000494:pharmacology*
D015703:Antigens, CD; D018952:Antigens, CD34; D017370:Interleukin-11; D007377:Interleukin-3; D007378:Interleukins; D011994:Recombinant Proteins