Secretion of urokinase and tissue-plasminogen activator by epidermal cells in the presence of psoriatic fibroblast-conditioned medium.
The present study examined secretion of urokinase and tissue-plasminogen activator by epidermal cells in the presence of psoriatic or uninvolved skin fibroblast-conditioned medium. Using zymographic analyses, a 54kD lysis band and a small 110kD band derived from urokinase could be detected in the harvest fluid from keratinocytes treated with both psoriatic and uninvolved fibroblast-conditioned medium, as well as very weak lysis bands of 63kD and 120kD derived from tissue-plasminogen activator in the harvest fluid treated with psoriatic fibroblast-conditioned medium, but not with uninvolved fibroblast-conditioned medium.
Department of Dermatology, Slade Hospital, Oxford, UK.
Y
Y
Horiuchi
J M
JM
Marshall
S A
SA
Cederholm-Williams
T J
TJ
Ryan
D000328:Adult; D002478:Cells, Cultured; D017077:Culture Media, Conditioned; D005347:Fibroblasts / Q000502:physiology; D006801:Humans; D015603:Keratinocytes / Q000201:enzymology* / Q000378:metabolism; D008970:Molecular Weight; D011565:Psoriasis / Q000201:enzymology*; D010959:Tissue Plasminogen Activator / Q000378:metabolism*; D014568:Urokinase-Type Plasminogen Activator / Q000378:metabolism*
D017077:Culture Media, Conditioned; D010959:Tissue Plasminogen Activator; D014568:Urokinase-Type Plasminogen Activator