The membrane dialysis bioreactor with integrated radial-flow fixed bed--a new approach for continuous cultivation of animal cells.
A hybridoma cell was cultivated continuously in a membrane dialysis bioreactor with an integrated radial-flow fixed bed consisting of porous Siran carriers over a period of 6 weeks. Antibodies accumulated to an average of 100 mg l-1, approx. 10 times more than in fixed bed cultures without dialysis membrane. Serum costs could be reduced about 85% due to an appropriate feeding strategy. Siran carriers with 3-5 mm diameter showed an advantage compared to those with 1-2 mm diameter. For the 3-5 mm carrier the specific glucose uptake rate and the MAb production rate were constant, if the velocity was between 0.09 mm s-1 and 0.75 mm s-1. At higher velocities cells are washed out of the bed. Furthermore antibody consistency and cell stability were verified in long-term cultivations over a period of 96 days. From an estimation of the antibody concentration reachable with the reactor concept under optimal conditions a concentration 45 times higher compared to axial-flow fixed bed reactors and 11 times higher compared to stirred tank reactors can be expected.
Technische Universität Hamburg-Harburg, Germany.
A
A
Bohmann
R
R
Pörtner
J
J
Schmieding
V
V
Kasche
H
H
Märkl
D000818:Animals; D001709:Biotechnology / Q000295:instrumentation*; D002460:Cell Line; D002875:Chromosomes; D003956:Dialysis; D005898:Glass; D006825:Hybridomas / Q000166:cytology*; D008567:Membranes, Artificial; D051379:Mice
D008567:Membranes, Artificial