Selective protein separations using Formed-In-Place anion exchange membranes.
Anion exchange membranes prepared by adsorption of polymers on Formed-In-Place microfiltration substrates were formed and ion-exchange separations of solutions containing two proteins were determined by ion exchange membrane sequential separation procedures, similar to affinity membrane separation procedures. Representative ion exchange separation processes utilizing adsorbed poly(ethylene imine) (PEI) as the ion exchange membrane for the separation of the components of solutions containing two proteins, bovine serum albumin (BSA) and lysozyme and ovalbumin and lysozyme, are described. The stability of the PEI adsorbed layer, binding characteristics of the BSA to the membrane and purification properties of the procedure were determined.
Department of Chemistry, Clemson University, South Carolina 29634-1905.
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D000327:Adsorption; D005374:Filtration; D007474:Ion Exchange; D008567:Membranes, Artificial; D010539:Permeability; D011094:Polyethyleneimine; D011485:Protein Binding; D011506:Proteins / Q000302:isolation & purification*
D008567:Membranes, Artificial; D011506:Proteins; D011094:Polyethyleneimine