MHC class II beta-chain expression in the rainbow trout.
A cDNA clone corresponding to the MHC class II beta-chain of the rainbow trout (Oncorhynchus mykiss) has been isolated and used in restriction fragment length polymorphism (RFLP) studies in a family of full siblings of rainbow trout. A very simple RFLP pattern was detected, suggesting segregation of a homozygote AA genotype and a heterozygote AB genotype. The MHC class II beta-chain of the rainbow trout seems to be transcribed in the same type of cells as class II genes of higher vertebrates even though the cDNA clone recognizes atypical messenger sizes of 2.2 kb and 3.6 kb in the analysed family. Surprisingly the transcripts seem to be allele-specific for the assigned genotypes.
Institute for Experimental Immunology, Copenhagen, Denmark.
H R
HR
Juul-Madsen
J
J
Glamann
H O
HO
Madsen
M
M
Simonsen
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002347:Carps; D002645:Chickens; D004247:DNA / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D005802:Genes, MHC Class II / Q000235:genetics*; D000949:Histocompatibility Antigens Class II / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D012333:RNA, Messenger / Q000235:genetics; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D014337:Trout / Q000235:genetics* / Q000276:immunology
D000949:Histocompatibility Antigens Class II; D012333:RNA, Messenger; D004247:DNA