A single 24h contact time with adriamycin provokes the emergence of resistant cells expressing the Gp 170 protein.
We investigate here, in A549 cells, the influence of a single short (1h) or long (24h) adriamycin (ADR) contact time on the long-term cytotoxicity of the drug and on the emergence of resistant cells. In contrast to a 1 h ADR contact, a 24 h treatment provokes the emergence of resistant cells overexpressing the Gp170 protein and this overexpression is maintained for at least three months without any drug selection pressure.
Chevillard
S
S
Institut Curie, Section de Biologie, Paris, France.
Vielh
P
P
Bastian
G
G
Coppey
J
J
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D002460:Cell Line; D004317:Doxorubicin / Q000494:pharmacology*; D004351:Drug Resistance; D006801:Humans; D008562:Membrane Glycoproteins / Q000032:analysis*; D013997:Time Factors
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D008562:Membrane Glycoproteins; D004317:Doxorubicin