Solid-phase extraction and GC/MS confirmation of barbiturates from human urine.
A highly selective and sensitive procedure has been developed for isolating and identifying barbiturates in human urine. With a new disposable bonded silica gel solid-phase extraction (SPE) column and hexobarbital as an internal standard (IS), amobarbital, butabarbital, pentobarbital, phenobarbital, secobarbital, and methaqualone were selectively isolated from endogenous urine components. Capillary gas chromatography/ion trap mass spectrometry (GC/MS) analysis of the extracts generated a full mass spectrum for the detection, identification, and quantitation of barbiturates. Linear quantitative response curves for the drugs have been generated over a concentration range of 20-500 ng/mL. Overall extraction efficiencies for drugs averaged greater than 90%, and the quantitative response curves exhibited correlation coefficients of 0.996 to 0.999.
Varian Sample Preparation Products, Harbor City, California 90710.
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D000654:Amobarbital / Q000652:urine; D001463:Barbiturates / Q000652:urine*; D002623:Chemistry Techniques, Analytical / Q000379:methods; D002845:Chromatography / Q000379:methods; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008702:Methaqualone / Q000652:urine; D010424:Pentobarbital / Q000652:urine; D010634:Phenobarbital / Q000652:urine; D015203:Reproducibility of Results; D012631:Secobarbital / Q000652:urine; D012680:Sensitivity and Specificity; D058428:Silica Gel; D012822:Silicon Dioxide
D001463:Barbiturates; D012631:Secobarbital; D058428:Silica Gel; D012822:Silicon Dioxide; D008702:Methaqualone; D000654:Amobarbital; D010424:Pentobarbital; D010634:Phenobarbital