The relative importance of the routes and sources of wound contamination during general surgery. II. Airborne.
The influence of airborne bacteria on wound contamination during biliary surgery was studied. When bacteria grew in the bile they accounted for most of the bacteria in the wound but when the wounds were free of bile bacteria many of the bacteria came from the patient's skin. It was only in wounds with little contamination from non-airborne routes that it was possible to demonstrate an effect of airborne contamination. In such a situation it was estimated that a reduction in the airborne bacteria in the operating room of about 13-fold would reduce the wound contamination by about 50%. The contamination of patient drapes from various sources and its relationship to wound contamination was studied. It was demonstrated that in areas away from the wound, the bacterial concentration on the drape surface was significantly affected only by airborne bacteria. In the area close to the wound, airborne bacteria and bacteria from the wound significantly affected drape contamination. However, it was found that more bacteria transferred from the wound to the drape surface than vice versa. Punctured gloves, impervious gowns and the number of bacteria on the patient's skin did not significantly affect the counts on the drapes' surfaces.
Building Services Research Unit, University of Glasgow, Scotland.
W
W
Whyte
A
A
Hambraeus
G
G
Laurell
J
J
Hoborn
D000391:Air Microbiology; D001419:Bacteria / Q000302:isolation & purification; D001646:Bile / Q000382:microbiology; D001662:Biliary Tract Surgical Procedures; D015169:Colony Count, Microbial; D006801:Humans; D009873:Operating Rooms; D012044:Regression Analysis; D012867:Skin / Q000382:microbiology; D013530:Surgical Wound Infection / Q000382:microbiology*; D014691:Ventilation