Determination of pipamperone in human plasma by high performance liquid chromatography.
A sensitive and reproducible high performance liquid chromatographic method was developed for the determination of pipamperone (1'-[4-(4-fluorophenyl)-4-oxobutyl]-[1,4'-bipiperidine]-4'- carboxamide, Dipiperon, CAS 1893-33-0) in plasma samples. Pipamperone and its internal standard (piritramide or 1'-(3-cyano-3,3-diphenylpropyl)-[1,4'-bipiperidine]-4'-carbo xamide) were extracted from alkalinized plasma by liquid-liquid extraction and analysed on a reversed-phase (C18) column. In order to reduce analysis time, a gradient elution technique was applied. Chromatographic peaks were quantified by UV detection at two wavelengths. The method has a detection limit of 2 ng/ml, it proved to have good accuracy and precision, and was linear in the range of 2 to 400 ng/ml. The assay was extensively applied to study the pharmacokinetics in man after oral administration of the drug.
Janssen Research Foundation, Janssen GmbH, Neuss, Fed. Rep. of Germany.
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Luhmann
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Szathmary
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Grünert
D014150:Antipsychotic Agents / Q000097:blood* / Q000493:pharmacokinetics; D001205:Ascorbic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D002090:Butyrophenones / Q000097:blood* / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid; D016166:Free Radical Scavengers; D006801:Humans; D011786:Quality Control; D012996:Solutions; D014810:Vitamin E / Q000031:analogs & derivatives* / Q000494:pharmacology
D014150:Antipsychotic Agents; D002090:Butyrophenones; D016166:Free Radical Scavengers; D012996:Solutions; D014810:Vitamin E; C005569:pipamperone; C066638:potassium ascorbyl tocopheryl phosphate; D001205:Ascorbic Acid