Elevated expression of ICAM1 (CD54) and minimal expression of LFA3 (CD58) in Epstein-Barr-virus-positive nasopharyngeal carcinoma cells.
Undifferentiated nasopharyngeal carcinoma (NPC) is a remarkable entity among human tumors because of its constant association with the Epstein-Barr virus (EBV). Malignant epithelial cells harbor the EBV genome and often express at least 2 species of latent EBV protein (EBNA1 and LMP1). Despite the massive presence of tumor-infiltrating lymphocytes, NPC cells obviously escape immune surveillance directed to EBV antigens. Previous investigations carried out on EBV-positive Burkitt lymphoma (BL) cells have shown that this fact may be partially accounted for by a lack of expression of ICAM1 (CD54) and LFA3 (CD58). ICAM1 and LFA3 have therefore been investigated in fresh NPC biopsies and transplanted NPCs. With only 1 exception out of 9 cases, NPC cells appear to express high levels of ICAM1 and low levels of LFA3. This is a complete inversion of the pattern observed in normal epithelial cells in vivo. Additional investigations will be required to determine to what extent these characteristics affect T-cell interactions with NPC cells, specially in the process of EBV-antigen recognition.
Laboratoire de Biologie des Tumeurs Humaines, URA 1156 CNRS, Institut Gustave Roussy, Villejuif, France.
P
P
Busson
Q
Q
Zhang
J M
JM
Guillon
C D
CD
Gregory
L S
LS
Young
B
B
Clausse
M
M
Lipinski
A B
AB
Rickinson
T
T
Tursz
D000818:Animals; D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000032:analysis*; D000954:Antigens, Surface / Q000032:analysis*; D018968:CD58 Antigens; D015815:Cell Adhesion Molecules / Q000032:analysis* / Q000235:genetics; D002460:Cell Line; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000302:isolation & purification*; D015395:Histocompatibility Antigens Class I / Q000032:analysis; D006801:Humans; D018799:Intercellular Adhesion Molecule-1; D008562:Membrane Glycoproteins / Q000032:analysis*; D051379:Mice; D008819:Mice, Nude; D009303:Nasopharyngeal Neoplasms / Q000276:immunology / Q000382:microbiology* / Q000473:pathology* / Q000601:surgery; D009368:Neoplasm Transplantation; D011991:Receptors, Virus / Q000032:analysis*; D014158:Transcription, Genetic; D014183:Transplantation, Heterologous; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000954:Antigens, Surface; D018968:CD58 Antigens; D015815:Cell Adhesion Molecules; D015395:Histocompatibility Antigens Class I; D008562:Membrane Glycoproteins; D011991:Receptors, Virus; D018799:Intercellular Adhesion Molecule-1