Characterization of putative neurotransmitter N-acetyl-aspartyl-glutamic acid and some related compounds by fast atom bombardment and tandem mass spectrometry.
The characterization of the N-blocked neuropeptide N-acetyl-aspartyl-glutamic acid and of some related compounds was carried out by negative-ion fast atom bombardment mass spectrometry and collision-induced dissociation of the generated [M-H]- species. Thorough analysis of the fragment ion spectra allowed discrimination between sequence isomeric compounds, and highlighting of differences between dipeptides linked in the normal or iso mode.
Rubino
F M
FM
Istituto di Tecnologie Biomediche Avanzate-CNR, Milano, Italy.
Zecca
L
L
Chillemi
F
F
D000595:Amino Acid Sequence; D001224:Aspartic Acid / Q000032:analysis; D001923:Brain Chemistry; D004151:Dipeptides / Q000032:analysis*; D005971:Glutamates / Q000032:analysis; D018698:Glutamic Acid; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D018377:Neurotransmitter Agents / Q000032:analysis*; D016339:Spectrometry, Mass, Fast Atom Bombardment
D004151:Dipeptides; D005971:Glutamates; D018377:Neurotransmitter Agents; C027172:isospaglumic acid; D001224:Aspartic Acid; D018698:Glutamic Acid