Intact chemoreceptor-based biosensors.
A receptrode biosensor is presented that uses intact chemoreceptor-based molecular recognition from antennular structures of the Hawaiian swimming crab species Portunis sanguinolentus. The sensor is coupled to a learning, pattern recognition calculation for performing analytical chemistry. Action potential waveforms are used to establish the identity of individual action potential types that can be associated to particular analytes. The pattern recognition calculations used are referred to as cluster analysis (CA) and principal component analysis (PCA). Action potential similarities are determined by using a dendrogram plot of the cluster analysis results and further elucidated by using principal component scores plots. Quantitative analysis was performed after classification of analyte and background responses. Chemoresponses to salinity and trimethylamine N-oxide, two chemical constituents that are found in the crustacean living environment, were investigated and gave analytic responses over several orders of magnitude.
Barker
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TQ
Hawaii Biosensor Laboratory, Department of Chemistry, University of Hawaii, Honolulu 96822.
Buch
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RM
Rechnitz
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GA
D000200:Action Potentials; D000818:Animals; D003386:Brachyura; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002628:Chemoreceptor Cells / Q000502:physiology; D005583:Fourier Analysis; D008433:Mathematics; D008744:Methylamines / Q000032:analysis; D012965:Sodium Chloride / Q000032:analysis
D008744:Methylamines; D012965:Sodium Chloride; C005855:trimethyloxamine