Establishment of the human BSMZ breast cancer cell line, which overexpresses the erbB-2 and c-myc genes.
A new cell line, designated BSMZ, was established from a malignant pleural effusion from a woman with breast cancer. This line has a doubling time of 27 h and has now been cultured for over 120 passages. The large, rounded BSMZ cells grow as both a monolayer and as aggregations in suspension. Intracytoplasmic lumen, a finding consistent with results from cells derived from mammary tissue, was detected on ultrastructural analysis. Injection of BSMZ cells into nude mice resulted in the growth of solid tumors 4 weeks after inoculation. The solid tumor was identical to the original BSMZ cells in microscopic and electron microscopic studies. These cells possess an average of 80 chromosomes. Expression of erbB-2 and c-myc genes was increased by 10-fold, while there was no detectable overexpression of the N-ras and c-myb genes. Southern analysis has revealed amplification of the erbB-2 and c-myc loci. The BSMZ cell line may therefore provide a useful model for the study of human breast cancer and overexpression of the erbB-2 gene.
Department of Microbiology, Jikei University School of Medicine, Tokyo, Japan.
M
M
Watanabe
H
H
Tanaka
M
M
Kamada
J H
JH
Okano
H
H
Takahashi
K
K
Uchida
A
A
Iwamura
M
M
Zeniya
T
T
Ohno
D000328:Adult; D000818:Animals; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology; D002455:Cell Division / Q000502:physiology; D005260:Female; D005784:Gene Amplification / Q000235:genetics; D015870:Gene Expression / Q000235:genetics*; D016259:Genes, myc / Q000235:genetics*; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D010996:Pleural Effusion / Q000473:pathology; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D018719:Erb-b2 Receptor Tyrosine Kinases; D014407:Tumor Cells, Cultured
D011518:Proto-Oncogene Proteins; D018719:Erb-b2 Receptor Tyrosine Kinases