Microtiter assay for measurement of factor XIII activity in plasma.
A sensitive, reproducible and rapid quantitative microtiter assay for factor XIII activity is described. The assay is based on incorporation of a soluble amine substrate into casein attached to polystyrol microtiter plates and detection of bound substrate with a monoclonal antibody-enzyme conjugate. Defibrination of plasma samples is not required. The assay shows strong correlation with the immunologic assay for factor XIII catalytic subunit a (r = 0.94), the factor XIII dansylcadaverine assay (r = 0.83), and the factor XIII clot solubility test. Normal values detected in healthy blood donors were in the range of 74% to 117%, with a mean value of 96.9% and a median value of 97.8%; pooled normal plasma was used as a reference. Since the assay is performed in a single microtiter plate and requires few reagents and short incubation periods, it can be easily adapted for clinical use.
Dempfle
C E
CE
University of Heidelberg, Faculty of Clinical Medicine Mannheim, First Dept. of Medicine, Germany.
Harenberg
J
J
Hochreuter
K
K
Heene
D L
DL
D002364:Caseins; D005176:Factor XIII / Q000032:analysis*; D005340:Fibrinogen; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D011503:Transglutaminases / Q000032:analysis
D002364:Caseins; D005340:Fibrinogen; D005176:Factor XIII; D011503:Transglutaminases