The effect of estrous cycle and buthionine sulfoximine on glutathione release from the in vitro perfused rat ovary.
There is little known regarding the intracellular mechanisms of modification of damage in the ovary. Ovarian perfusion of en block dissections of the rat right ovary with aorta and vena cava were done to determine (a) if glutathione (GSH) is released by the ovary, (b) if the release is cycle dependent, and (c) if GSH released is the product of de novo ovarian synthesis. All perfused ovaries released GSH and the release was maximal at estrus and least at metestrus. Perfusion with buthionine sulfoximine, a specific inhibitor of gamma-glutamylcysteine synthetase, resulted in a dose-dependent reduction in GSH released, indicating inhibition of de novo synthesis during perfusion.
Department of Obstetrics & Gynaecology, University of Calgary, Alberta, Canada.
N
N
Clague
M
M
Sevcik
G
G
Stuart
M
M
Brännström
P O
PO
Janson
J F
JF
Jarrell
D000818:Animals; D019328:Buthionine Sulfoximine; D004971:Estrus / Q000502:physiology*; D005260:Female; D005721:Glutamate-Cysteine Ligase / Q000037:antagonists & inhibitors; D005978:Glutathione / Q000378:metabolism*; D066298:In Vitro Techniques; D007773:Lactates / Q000378:metabolism; D019344:Lactic Acid; D008717:Methionine Sulfoximine / Q000031:analogs & derivatives* / Q000494:pharmacology; D010053:Ovary / Q000187:drug effects* / Q000378:metabolism; D010477:Perfusion; D011773:Pyruvates / Q000378:metabolism; D019289:Pyruvic Acid; D051381:Rats; D017207:Rats, Sprague-Dawley
D007773:Lactates; D011773:Pyruvates; D008717:Methionine Sulfoximine; D019344:Lactic Acid; D019328:Buthionine Sulfoximine; D019289:Pyruvic Acid; D005721:Glutamate-Cysteine Ligase; D005978:Glutathione