Modular integrated fluidized bed bioreactor technology.
We describe the design and demonstrate the application of a modular integrated fluidized bed bioreactor system. Basically the system is a reactor vessel equipped with an extending cylinder and a liquid distributor plate. Instead of an external recirculation loop, as used in existing fluidized bed systems, a low shear stress impeller is used as the recirculation pump. The system has several unique features, such as modular exchangeable elements, efficient oxygenation and the option of operating as a stirred tank-, a packed bed- or a fluidized bed reactor. An example of a fluidized bed run using CHO-K1 cells is shown. Under standard culture conditions a 100-fold increase in cell density (up to 1.2 x 10(8) cells/ml) was achieved.
Institute of Applied Microbiology (IAM), University of Agriculture, Vienna, Austria.
M
M
Reiter
G
G
Blüml
T
T
Gaida
N
N
Zach
F
F
Unterluggauer
O
O
Doblhoff-Dier
M
M
Noe
R
R
Plail
S
S
Huss
H
H
Katinger
D000818:Animals; D001709:Biotechnology / Q000295:instrumentation* / Q000379:methods; D016466:CHO Cells; D006224:Cricetinae; D046508:Culture Techniques / Q000295:instrumentation* / Q000379:methods; D015483:HIV Antibodies / Q000235:genetics*; D015497:HIV-1 / Q000276:immunology; D006801:Humans; D011995:Recombination, Genetic
D015483:HIV Antibodies