Processing in the 5' region of the pnp transcript facilitates the site-specific endonucleolytic cleavages of mRNA.
The primary transcript of pnp, the gene encoding polynucleotide phosphorylase in Escherichia coli, is processed in the 5' end region by ribonuclease III (RNase III). The unprocessed transcript shows enhanced stability compared with the processed transcript. We report here that, unlike the processed transcript, the unprocessed pnp transcript did not accept endonucleolytic attack at, at least, five cleavage sites. Sequencing analysis of the four cleavage products shows no sequence specific to all these sites, but AU rich stretches were observed at three sites.
Takata
R
R
Department of Biotechnology, College of Agriculture, Ehime University, Japan.
Izuhara
M
M
Akiyama
K
K
D001483:Base Sequence; D004722:Endoribonucleases / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D029968:Escherichia coli Proteins; D008969:Molecular Sequence Data; D011117:Polyribonucleotide Nucleotidyltransferase / Q000235:genetics*; D012322:RNA Precursors / Q000378:metabolism*; D012323:RNA Processing, Post-Transcriptional / Q000235:genetics*; D012329:RNA, Bacterial / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D043244:Ribonuclease III
D029968:Escherichia coli Proteins; D012322:RNA Precursors; D012329:RNA, Bacterial; D012333:RNA, Messenger; D011117:Polyribonucleotide Nucleotidyltransferase; D004722:Endoribonucleases; D043244:Ribonuclease III; C468366:ribonuclease III, E coli