Distribution of 111In-bleomycin complex in small cell lung cancer cells by autoradiography.
The distribution of 111In-bleomycin Complex (111In-BLMC) in small cell lung cancer (SCLC) cells was studied by autoradiography. SCLC cells were exposed to 111In-BLMC and 111Indium chloride (111InCl3) for 1 hour, 3 hours, and 4 hours; washed with fresh medium; and spread on slides. The slides were smeared with NTB2 (NTB3) emulsion by wet or dry-mount technique and exposed 3 to 15 days. 111In-BLMC was found to localize in the cell nucleus and nuclear membrane (78.3%); 111InCl3 located mainly in the cytoplasm (52.3%). This distribution of labeled BLM may explain the mechanism of killing SCLC cells by 111In-BLMC.
Department of Radiation Medicine, University of Kentucky Medical Center, Lexington.
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Hou
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Maruyama
D001345:Autoradiography; D001761:Bleomycin / Q000378:metabolism*; D018288:Carcinoma, Small Cell / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D006801:Humans; D007204:Indium / Q000378:metabolism; D007205:Indium Radioisotopes / Q000378:metabolism*; D008175:Lung Neoplasms / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D007205:Indium Radioisotopes; D007204:Indium; D001761:Bleomycin; C020758:indium trichloride