Isolation and sequencing of a gene, C-ADE1, and its use for a host-vector system in Candida maltosa with two genetic markers.
The host-vector systems of an n-alkane-assimilating-yeast, Candida maltosa, that we previously constructed consisted of a vector replicating with an ARS region of this yeast, and C. maltosa strains J288 (leu2) or CH1 (his5) as hosts. Since each of these hosts has a single genetic marker, we have developed a new host-vector system using two genetic markers. By UV irradiation of strain CH1, an adenine auxotrophic mutant, CHA1, forming red colonies was isolated. A DNA fragment complementing this deficiency was isolated from the C. maltosa genome. Since the DNA fragment also complemented the ade1 mutation of S. cerevisiae, we termed a gene contained in this DNA fragment C-ADE1. The nucleotides of C-ADE1 were sequenced. The deduced amino acid sequence (291 residues) had 65.6% homology with that of ADE1 of S. cerevisiae (306 residues). Having the cloned C-ADE1 DNA, we improved the host-vector system of C. maltosa.
Kawai
S
S
Department of Agricultural Chemistry, University of Tokyo, Japan.
Hikiji
T
T
Murao
S
S
Takagi
M
M
Yano
K
K
D000225:Adenine / Q000378:metabolism; D000595:Amino Acid Sequence; D001483:Base Sequence; D002175:Candida / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D005800:Genes, Fungal; D005819:Genetic Markers / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D016366:Open Reading Frames; D015183:Restriction Mapping; D014170:Transformation, Genetic / Q000502:physiology
D005819:Genetic Markers; D000225:Adenine