The synthesis of new xylosyloligosaccharides by transxylosylation with Aspergillus niger beta-xylosidase.
The synthesis of xylosyloligosaccharides from beta-(1----4)-xylobiose in the presence of D-mannose by transxylosylation with beta-xylosidase from Aspergillus niger IFO 6662 was studied. At first, the transxylosylation products were separated by charcoal column chromatography in 5% ethanol elution into three peaks, P-1, P-2, and P-3. They were further separated on thin layer chromatography, and their three products, saccharides O-4, O-5, and O-N were purified by preparative paper chromatography. Saccharides O-4 and O-5 were identified as O-beta-D-xylopyranosyl-(1----4)-D- mannopyranose and O-beta-D-xylopyranosyl-(1----6)-D-mannopyranose, respectively, by measurement of the degree of polymerization, specific rotation, acid hydrolysis, and methylation analysis. By 1H-NMR spectroscopy in addition to these analyses, saccharide O-N was identified as O-beta-D-xylopyranosyl-(1----1')-beta-D-xylopyranose, a new nonreducing xylobiose.
Institute of Applied Biochemistry, University of Tsukuba, Ibaraki, Japan.
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Kizawa
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Shinoyama
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Yasui
D001234:Aspergillus niger / Q000201:enzymology*; D002240:Carbohydrate Sequence; D002606:Charcoal; D002845:Chromatography; D002851:Chromatography, High Pressure Liquid; D002854:Chromatography, Paper; D002855:Chromatography, Thin Layer; D004187:Disaccharides / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D008358:Mannose / Q000378:metabolism; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000138:chemical synthesis* / Q000302:isolation & purification; D014995:Xylosidases / Q000378:metabolism*
D004187:Disaccharides; D009844:Oligosaccharides; D002606:Charcoal; D014995:Xylosidases; C026579:exo-1,4-beta-D-xylosidase; C004173:xylobiose; D008358:Mannose