The protective role of polyphenols in cytotoxicity of hydrogen peroxide.
A variety of synthetic and natural polyphenols protect mammalian cells from hydrogen peroxide (H2O2). Cytotoxicity of H2O2 on Chinese hamster V79 cells was assessed with a colony formation assay, and several polyphenols prevented the decrease in the number of colonies caused by H2O2. A study of the structure-activity relationship revealed that affinity of the polyphenols for the cell membrane and the presence of an ortho-dihydroxy moiety in their structure proved essential to this protection.
Department of Food Science and Technology, Faculty of Agriculture, Nagoya University, Japan.
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Nakayama
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Niimi
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Osawa
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Kawakishi
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002460:Cell Line; D002462:Cell Membrane / Q000187:drug effects; D003114:Colony-Forming Units Assay; D006224:Cricetinae; D005419:Flavonoids; D006861:Hydrogen Peroxide / Q000037:antagonists & inhibitors / Q000633:toxicity*; D010636:Phenols / Q000494:pharmacology*; D011108:Polymers / Q000494:pharmacology*; D059808:Polyphenols; D013329:Structure-Activity Relationship
D000975:Antioxidants; D005419:Flavonoids; D010636:Phenols; D011108:Polymers; D059808:Polyphenols; D006861:Hydrogen Peroxide