Emperipolesis of lymphoid cells by human follicular dendritic cells in vitro.
Isolated follicular dendritic cells (FDCs) showed true and pseudoemperipolesis of fresh tonsillar lymphocytes, even after long-term (50-day) cultivation. Emperipolesis by FDCs was not restricted by allotype specificity, nor was it inhibited by the addition of antibodies against MHC-I & II antigens. Follicular dendritic cells predominantly engulfed B-cells; monocytes and macrophages were not found between FDC cytoplasmic extensions. When highly purified T-cell populations were added to FDC cultures emperipolesis of T-cells occurred, particularly those of the CD4-positive phenotype. Mitoses appeared within 6 h in the emperipolesed lymphocytes and, after an additional 18 h, some lymphocytes exhibited apoptosis.
Department of Anatomy Histology, Fukushima Medical College, Japan.
R
R
Tsunoda
M
M
Nakayama
E
E
Heinen
K
K
Miyake
K
K
Suzuki
N
N
Sugai
M
M
Kojima
D000943:Antigens, Differentiation / Q000378:metabolism; D002448:Cell Adhesion / Q000502:physiology; D003713:Dendritic Cells / Q000276:immunology / Q000502:physiology* / Q000648:ultrastructure; D006801:Humans; D007124:Immunoenzyme Techniques; D066298:In Vitro Techniques; D008214:Lymphocytes / Q000502:physiology / Q000648:ultrastructure*; D008853:Microscopy; D008858:Microscopy, Phase-Contrast; D014066:Palatine Tonsil / Q000166:cytology
D000943:Antigens, Differentiation