[The effect of epidermal growth factor on the expression of erb B2/neu oncogene in mouse embryo fibroblast cells].
The erb B2/neu oncogene encodes a protein which sequence is closely similar to the epidermal growth factor receptor (EGFR). We have previously found that EGF can induce the expression of erb B1/EGFR gene in normal and 3H-TdR transformed C3H/10T1/2CL8 mouse embryo fibroblast cells i.e. NC3H10 and TC 3H10 respectively, but we do not know whether the neu oncogene expression can be induced by EGF. In this study, the effect of EGF on NC3H10 and TC3H10 has been observed by Northern blot analysis. The result indicated that EGF had a obvious induction effect on neu oncogene expression in these cells. Thus, the expression of both erbB 1/EGFR gene and erbB 2/neu oncogene can be induced by EGF. This result may provide a novel clue to the molecular mechanism of EGF action in cell nucleus.
Zheng
Y S
YS
Department of Biochemistry, Beijing Medical University.
Tong
T J
TJ
D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D005333:Fetus; D005347:Fibroblasts / Q000166:cytology; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D051379:Mice; D011518:Proto-Oncogene Proteins / Q000187:drug effects / Q000235:genetics*; D018719:Erb-b2 Receptor Tyrosine Kinases
D004815:Epidermal Growth Factor; D011518:Proto-Oncogene Proteins; D018719:Erb-b2 Receptor Tyrosine Kinases