An integrated process for the production and biotransformation of penicillin.
The extraction of Penicillin G (PG) from the filtered cultivation medium of Penicillium chrysogenum and its conversion into 6-amino penicillanic acid (6-APA) and phenyl acetic acid (PhA) at pH 8 was performed in a 10 l kühni extractor during the production by means of penicillin-G-amidase immobilized in a liquid membrane carrier system (LM). 6-APA was enriched in LM, and the PhA returned to the cultivation medium. After electrocoalescence of LM, the 6-APA was converted into ampicillin with the same enzyme at pH 6, while the liquid membrane phase and enzyme were recycled and reused.
Institut für Technische Chemie, Universität Hannover, Germany.
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D000667:Ampicillin / Q000302:isolation & purification / Q000378:metabolism; D001711:Biotransformation; D003470:Culture Media; D004800:Enzymes, Immobilized; D008567:Membranes, Artificial; D010397:Penicillanic Acid / Q000378:metabolism / Q000031:analogs & derivatives; D010406:Penicillins / Q000096:biosynthesis* / Q000302:isolation & purification / Q000493:pharmacokinetics*; D010408:Penicillium chrysogenum / Q000737:chemistry / Q000254:growth & development / Q000378:metabolism*; D010539:Permeability
D003470:Culture Media; D004800:Enzymes, Immobilized; D008567:Membranes, Artificial; D010406:Penicillins; D000667:Ampicillin; D010397:Penicillanic Acid; C004408:aminopenicillanic acid