[Preparation and evaluation of coagglutination reagents for the identification of Vibrio cholerae 01. Its trial during a cholera outbreak in Minatitlán, Veracruz].
This study was realized in Minatitlán, Veracruz during a cholera outbreak. 169 rectal swabs were taken from hilles and their contacts. They were transfer alkaline peptone water for enrichment to V. cholerae and incubated for 8 hrs to 37 degrees C, 70 were positive for V. cholerae in both techniques. The coagglutination was done with a reagent prepared at the Instituto de Diagnóstico y Referencia Epidemiológicos of México and the culture were also performed in the same Institute. We obtained 100% of sensitivity and specificity of co-agglutination in relation with culture. This results gave the possibility to use this kind or reagents for a rapid presumptive diagnosis of cholera.
C
C
González Bonilla
A
A
Villanueva Zamudio
G
G
Bolaños Monrroy
S
S
Giono Cerezo
J L
JL
Valdespino Gómez
D000372:Agglutination Tests; D001706:Biopsy; D002771:Cholera / Q000175:diagnosis / Q000453:epidemiology / Q000382:microbiology*; D004196:Disease Outbreaks; D006801:Humans; D008800:Mexico; D012007:Rectum / Q000382:microbiology / Q000473:pathology; D012680:Sensitivity and Specificity; D013211:Staphylococcus aureus; D014734:Vibrio cholerae / Q000302:isolation & purification*
Preparación y evaluación del reactivo de coaglutinación para la identificación de Vibrio cholerae 01. Su ensayo durante un brote de cólera en Minatitlán, Veracruz.