Poliovirus chimeras expressing sequences from the principal neutralization domain of human immunodeficiency virus type 1.
Sequences from the principal neutralization domain of human immunodeficiency virus type 1 (HIV-1) strain LAI or RF have been expressed in antigenic site 1 of the capsid of the Sabin strain of poliovirus type 1. A number of the resulting chimeras were viable. Viable variants bearing mutations within the insertion site spontaneously arose from several nonviable chimeras. In general, these mutations result in a decrease in positive charge in the substituted antigenic site 1. Two of the chimeras were genetically stable and have been further characterized. Both chimeras were neutralized by various HIV-1 neutralizing antibodies. In rabbits, both chimeras produced high levels of antibodies which react with HIV-1 gp120/160 in immunoprecipitation and enzyme-linked immunosorbent assays. One of the chimeras (HIV-1LAI) produced a significant but weak HIV-1 neutralizing response.
Unité de Virologie Moléculaire (Centre National de la Recherche Scientifique UA 545), Institut Pasteur, Paris, France.
J F
JF
Dedieu
J
J
Ronco
S
S
van der Werf
J M
JM
Hogle
Y
Y
Henin
M
M
Girard
D000163:Acquired Immunodeficiency Syndrome / Q000235:genetics / Q000276:immunology*; D000595:Amino Acid Sequence; D000917:Antibody Formation; D001483:Base Sequence; D002678:Chimera; D000939:Epitopes / Q000235:genetics / Q000276:immunology*; D005822:Genetic Vectors; D015699:HIV Envelope Protein gp120 / Q000235:genetics / Q000276:immunology*; D015497:HIV-1 / Q000235:genetics / Q000276:immunology*; D006801:Humans; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D017955:Poliovirus / Q000235:genetics / Q000276:immunology*; D015202:Protein Engineering
D000939:Epitopes; D015699:HIV Envelope Protein gp120