Immunosuppressive factor from liver and its influence on T cell development.
An endogenous immunosuppressive factor from liver, named ISF-70, of 70 kDa was studied with respect to mouse thymocyte maturation and expression of differentiation antigens in vitro. This factor induced dose-dependent alternative changes in thymocyte viability curves for 72 h incubation. Augmentation of viable cells was accompanied by a decrease in DNA synthesis and an increase in Lyt-2-positive cells. A correlation was observed between thymocyte proliferation, Lyt-2 expression and the action of ISF-70. It was found that ISF-70 distribution on thymocytes coincided with that of Lyt-2 antigen. The role of this factor in thymocyte developmental pathways and self-tolerance is discussed.
Institute of Immunology, Moscow, Russia.
D
D
Kazansky
N
N
Nastoyashchaya
M
M
Lomakin
N
N
Artsimovich
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis; D000950:Antigens, Ly / Q000032:analysis; D000954:Antigens, Surface / Q000276:immunology; D001685:Biological Factors / Q000494:pharmacology*; D002470:Cell Survival; D002478:Cells, Cultured; D004261:DNA Replication / Q000187:drug effects; D005260:Female; D008099:Liver / Q000737:chemistry*; D008562:Membrane Glycoproteins / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL / Q000276:immunology; D008808:Mice, Inbred CBA / Q000276:immunology; D016176:T-Lymphocyte Subsets / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D018800:Thy-1 Antigens; D013950:Thymus Gland / Q000166:cytology
D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D000950:Antigens, Ly; D000954:Antigens, Surface; D001685:Biological Factors; D008562:Membrane Glycoproteins; D018800:Thy-1 Antigens; C077784:immunosuppressive factor-neutral protein, mouse