The use of dyes in protein purification.
The role of the matrix, ligand and linking mechanism in affinity chromatography is discussed, special emphasis being placed on the use of dyestuff molecules as ligands. Current knowledge of dye-protein interactions is outlined and problems arising from the use of conventional textile dyes as ligands are considered. Work on the synthesis of novel dye-like molecules designed specifically for affinity chromatography is reviewed. This is seen as leading to the development of improved affinity systems capable of advancing the utility of affinity chromatography in protein purification.
Affinity Chromatography Ltd., Freeport, Ballasalla, Isle of Man.
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Stead
D002846:Chromatography, Affinity; D004396:Coloring Agents; D008024:Ligands; D011506:Proteins / Q000302:isolation & purification*
D004396:Coloring Agents; D008024:Ligands; D011506:Proteins