Thy-1 modulation and cell proliferation at early steps of intrathymic bone marrow cell differentiation.
Intrathymic (IT) transfer of bone marrow (BM) precursor cells in sublethally irradiated hosts has been widely used to study T cell differentiation and maturation. In this report we have used double congenic mice Ly 5.1 Thy 1.1 (host) and Ly 5.2 Thy 1.2 (donor) and detected cycling Ly 5.2+ BM cells by in vivo bromodeoxyuridine incorporation, before induction of the Thy 1.2 antigen. Until Day 9 post-transfer, some donor type cells express a high level of Thy 1.2 together with macrophage and granulocyte markers. A few days later, a Thy 1.2low population transiently B220+ was detected. Thereafter, donor type cells expressed an intermediate Thy 1.2 brightness; this population then persisted and surpassed the other subsets. Our findings permitted to establish a relationship between cell cycle and Thy 1 fluorescence intensity according to the sequence: Thy 1low resting, Thy 1low cycling, Thy 1high cycling, Thy 1high resting. Moreover, we have shown that cells from the myeloïd and B lineages can, in vivo, transiently express the Thy 1 antigen, develop and differentiate within the thymus microenvironment.
INSERM U 345, CHU Necker (6 étage), Paris, France.
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D000818:Animals; D000954:Antigens, Surface / Q000096:biosynthesis*; D016026:Bone Marrow Transplantation; D002453:Cell Cycle; D004247:DNA / Q000096:biosynthesis; D005819:Genetic Markers; D008213:Lymphocyte Activation / Q000276:immunology*; D008562:Membrane Glycoproteins / Q000096:biosynthesis*; D051379:Mice; D008810:Mice, Inbred C57BL; D013601:T-Lymphocytes / Q000166:cytology* / Q000378:metabolism; D018800:Thy-1 Antigens; D013997:Time Factors
D000954:Antigens, Surface; D005819:Genetic Markers; D008562:Membrane Glycoproteins; D018800:Thy-1 Antigens; D004247:DNA