Differential collagen and fibronectin production by Thy 1+ and Thy 1- lung fibroblast subpopulations.
Pulmonary fibrosis resulting from diverse etiologies is characterized by proliferation of fibroblasts and excessive accumulation of interstitial collagen. Whether fibrosis is associated with selective expansion of fibroblast subpopulations differing in amounts or types of collagens synthesized is unknown. We have previously isolated lines and clones of normal murine lung fibroblasts based on the presence of the Thy 1 surface antigen. These subpopulations differ in morphology, growth characteristics, and display of class II major histocompatibility complex antigens (R.P. Phipps, D.P. Penney, P. Keng, H. Quill, A. Paxhia, S. Derdak, and M. E. Felch. Am. J. Respir. Cell Mol. Biol. 1: 65-74, 1989). We evaluated the amounts and types of collagen and fibronectin synthesized by Thy 1+ (Fib2-T-3+) and Thy 1- (Fib2-T-4-) lung fibroblast lines and clones. Thy 1+ fibroblast line synthesized two- to threefold more collagen and noncollagen protein than the Thy 1- line. In contrast, both the Thy 1+ and Thy 1- lines synthesized similar amounts of fibronectin. Thy 1+ and Thy 1- lines and clones expressed mRNA for alpha 1(I)-and alpha 1(III)-procollagen and synthesized both types (predominantly type I and lesser amounts of type III) of collagen, protein, and mRNA. The fibroblast clones varied significantly in total collagen and fibronectin production, with one Thy 1- clone (D3) synthesizing the largest amount of collagen but relatively little fibronectin.(ABSTRACT TRUNCATED AT 250 WORDS)
University of Rochester Cancer Center, Department of Medicine, New York.
S
S
Derdak
D P
DP
Penney
P
P
Keng
M E
ME
Felch
D
D
Brown
R P
RP
Phipps
D000818:Animals; D000954:Antigens, Surface / Q000032:analysis*; D003094:Collagen / Q000096:biosynthesis* / Q000235:genetics; D005347:Fibroblasts / Q000276:immunology / Q000378:metabolism; D005353:Fibronectins / Q000096:biosynthesis*; D008168:Lung / Q000166:cytology / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000032:analysis*; D010641:Phenotype; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism; D018800:Thy-1 Antigens
D000954:Antigens, Surface; D005353:Fibronectins; D008562:Membrane Glycoproteins; D012333:RNA, Messenger; D018800:Thy-1 Antigens; D003094:Collagen