[Restriction fragment length polymorphism of the 5'-region of the bovine ribosomal spacer repeat].
The restriction map of bovine 28S rRNA gene and adjacent 5'-spacer region was determined. The high level of intragenomic and population length polymorphism of EcoRI-BamHI restriction fragment was demonstrated to originated from the 3'-end of 28S rDNA and 5'-spacer of rDNA repeat. This polymorphism is more pronounced than the ones revealed in human and murine rDNA repeats and could be compared with genomic fingerprints obtained by M13 or minisatellite DNA hybridization probes. From family blot-panel analysis we concluded that in progeny only parental sets of length variants were inherited and that the copy number of definite variants does not change in the progeny as well. From these results it was proposed that the definite sets of linked in genome length variants are inherited independently from each other.
Morzunov
S P
SP
Gogolevskaia
I K
IK
Kupriianova
N S
NS
Timofeeva
M Ia
MIa
D000483:Alleles; D000818:Animals; D002417:Cattle; D003001:Cloning, Molecular; D004275:DNA, Ribosomal / Q000235:genetics; D016678:Genome; D012150:Polymorphism, Restriction Fragment Length; D012339:RNA, Ribosomal, 28S / Q000235:genetics*; D015183:Restriction Mapping
D004275:DNA, Ribosomal; D012339:RNA, Ribosomal, 28S
Polimorfizm dlin restriktaznykh fragmentov 5'-oblasti speĭsera tibosomnogo povtora byka.