Generation of a subtype-specific neutralization epitope in foot-and-mouth disease virus of a different subtype.
An epitope involved in neutralization of foot-and-mouth disease virus (FMDV) of subtype C3 was generated by a single amino acid replacement in VP1 of FMDV of subtype C1. The replacement [Ser (139)----Ile, in the immunodominant site A] was consistently found in those FMDV C1 Santa Pau-Sp/70 mutants resistant to neutralization by monoclonal antibody (MAb) SD6 (specific for most C1 viruses) that acquired the capacity to be neutralized by MAb 7AB5 (specific for C3 viruses).
Hernández
J
J
Centro de Biología Molecular, Universidad Autónoma de Madrid, Spain.
Martínez
M A
MA
Rocha
E
E
Domingo
E
E
Mateu
M G
MG
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal; D000956:Antigens, Viral / Q000737:chemistry / Q000276:immunology*; D005537:Aphthovirus / Q000737:chemistry / Q000276:immunology*; D000939:Epitopes / Q000737:chemistry / Q000276:immunology*; D008969:Molecular Sequence Data; D009154:Mutation; D009500:Neutralization Tests; D010948:Viral Plaque Assay
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D000939:Epitopes