Structure and expression of a plastid-encoded groEL homologous heat-shock gene in a thermophilic unicellular red alga.
A gene homologous to the E. coli groEL locus was identified on the plastid genome of the unicellular red alga Cyanidium caldarium strain 14-1-1 (synonym: Galdieria sulphuraria). The complete nucleotide sequence was determined and compared to bacterial- and nuclear-encoded counterparts of higher plants. At the amino-acid level the C. caldarium gene shows 70% homology to the corresponding gene of the cyanobacterium Synechococcus and 52% homology to nuclear-encoded counterparts of higher plants, respectively. Northern and Western blot experiments were used to investigate the dependence of the transcript- and protein-level on culture temperature and heat shock.
Institut für Pflanzenphysiologie, Justus Liebig Universität, Giessen, Federal Republic of Germany.
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Maid
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Steinmüller
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Zetsche
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D015152:Blotting, Northern; D015153:Blotting, Western; D018834:Chaperonin 60; D002736:Chloroplasts; D015342:DNA Probes / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression / Q000235:genetics; D006360:Heat-Shock Proteins / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics*; D015183:Restriction Mapping; D000461:Rhodophyta / Q000235:genetics*; D013696:Temperature
D001426:Bacterial Proteins; D018834:Chaperonin 60; D015342:DNA Probes; D006360:Heat-Shock Proteins; D010940:Plant Proteins; C075415:groEL protein, Cyanidium caldarium