POU-specific domain of Oct-2 factor confers 'octamer' motif DNA binding specificity on heterologous Antennapedia homeodomain.
The bipartite DNA binding domain of the POU family of transcription factors contains a 'POU-specific' domain unique to this class of factors and a 'POU homeodomain' homologous to other homeodomains. We compared DNA binding of the Oct-2 factor POU domain and the Antennapedia (Antp) homeodomain with a chimeric Oct-2/Antp protein in which the distantly related Antp homeodomain was substituted for the Oct-2 POU homeodomain. The Oct-2/Antp chimeric protein bound both the octamer and the Antp sites efficiently, indicating that DNA binding specificity is contributed by both components of the POU domain.
Brugnera
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Institute of Molecular Biology II, University of Zürich, Switzerland.
Xu
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Schaffner
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Arnosti
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D051943:Antennapedia Homeodomain Protein; D001483:Base Sequence; D001665:Binding Sites; D004247:DNA / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D003850:Deoxyribonuclease I; D005801:Genes, Homeobox; D018398:Homeodomain Proteins; D008745:Methylation; D008969:Molecular Sequence Data; D009687:Nuclear Proteins; D050813:Octamer Transcription Factor-2; D050809:POU Domain Factors; D011485:Protein Binding; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors
D051943:Antennapedia Homeodomain Protein; D004268:DNA-Binding Proteins; D018398:Homeodomain Proteins; D009687:Nuclear Proteins; D050813:Octamer Transcription Factor-2; D050809:POU Domain Factors; C058435:POU2F2 protein, human; D011993:Recombinant Fusion Proteins; D014157:Transcription Factors; D004247:DNA; D003850:Deoxyribonuclease I