Early and late replicative chromosomal banding patterns of Gallus domesticus.
Early and late replicating chromosomal banding patterns of Gallus domesticus were investigated by cell synchronization and incorporation of 5'-bromodeoxyuridine during early and late DNA synthesis. The early replicating chromosomal banding patterns observed, as revealed by either acridine orange or Hoechst 33258/propidium iodide staining, were similar to the structural G-banding patterns obtained by trypsin digestion and Giemsa staining. Late replicating chromosomal banding showed extensive reverse band complementarity to the G-banding pattern. Cell synchronization increased the number of prometaphase and metaphase plates available for analysis. G-banding obtained by Hoechst 33258/propidium iodide staining was investigated due to the fact that it is compatible with chromosomal in situ hybridization procedures that use nonisotopically-labeled DNA probes. Standard replicative G-banded and R-banded idiograms, as obtained after cell synchronization, are proposed.
Ponce de Leon
F A
FA
Department of Veterinary and Animal Sciences, University of Massachusetts, Amherst 01003.
Li
Y
Y
Weng
Z
Z
D000165:Acridine Orange; D000818:Animals; D006690:Bisbenzimidazole; D001973:Bromodeoxyuridine; D002460:Cell Line; D002642:Chick Embryo; D002645:Chickens / Q000235:genetics*; D002871:Chromosome Banding / Q000379:methods / Q000662:veterinary*; D005347:Fibroblasts; D007091:Image Processing, Computer-Assisted; D011419:Propidium; D016196:S Phase; D012730:Sex Chromosomes
D011419:Propidium; D000165:Acridine Orange; D001973:Bromodeoxyuridine; D006690:Bisbenzimidazole