A double-label analysis demonstrating the non-coexistence of tyrosine hydroxylase-like and GABA-like immunoreactivities in amacrine cells of the larval tiger salamander retina.
Previous studies have localized tyrosine hydroxylase, the rate-limiting enzyme for the production of dopamine, and gamma-aminobutyric acid (GABA) to amacrine cell populations in the larval tiger salamander retina. Double-label immunocytochemistry was used to examine if tyrosine hydroxylase-like and GABA-like immunoreactivities colocalize in tiger salamander amacrine cells. A total of 2,162 tyrosine hydroxylase-like immunoreactive amacrine cells were observed in double-labelled sections. None of these cells were observed to express GABA-like immunoreactivity. Therefore, the present study demonstrates that dopamine and GABA are localized to distinct neuronal populations in the larval tiger salamander retina.
Alice R. McPherson Laboratory of Retina Research, Baylor College of Medicine, The Woodlands, TX 77381.
C B
CB
Watt
V J
VJ
Florack
D000818:Animals; D005455:Fluorescent Antibody Technique; D007150:Immunohistochemistry; D007814:Larva; D012160:Retina / Q000166:cytology*; D014446:Tyrosine 3-Monooxygenase / Q000032:analysis*; D014562:Caudata; D005680:gamma-Aminobutyric Acid / Q000032:analysis*
D005680:gamma-Aminobutyric Acid; D014446:Tyrosine 3-Monooxygenase