Alzheimer skin fibroblasts show increased susceptibility to free radicals.
We have studied the response to toxic oxygen metabolites of fibroblasts derived from skin biopsies of 5 patients with familial (FAD) and 4 with sporadic (AD) Alzheimer's disease compared with those derived from 4 normal controls. Fibroblasts were damaged by the generation of oxygen metabolites during the enzymatic oxidation of acetaldehyde by 50 munits of xanthine-oxidase (Xo). To quantify cell damage we measured lactate dehydrogenase (LDH) activity in the culture medium and cell viability in fibroblast cultures. We found a significant increase in LDH activity in the FAD vs. controls and also in the AD vs. controls.
Tesco
G
G
Department of Neurological and Psychiatric Sciences, University of Florence, Italy.
Latorraca
S
S
Piersanti
P
P
Piacentini
S
S
Amaducci
L
L
Sorbi
S
S
D000079:Acetaldehyde / Q000378:metabolism; D000375:Aging / Q000378:metabolism; D000544:Alzheimer Disease / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D002470:Cell Survival; D005347:Fibroblasts / Q000378:metabolism; D016166:Free Radical Scavengers; D005609:Free Radicals; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism*; D012867:Skin / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000378:metabolism; D014969:Xanthine Oxidase / Q000378:metabolism
D016166:Free Radical Scavengers; D005609:Free Radicals; D017382:Reactive Oxygen Species; D007770:L-Lactate Dehydrogenase; D013482:Superoxide Dismutase; D014969:Xanthine Oxidase; D000079:Acetaldehyde