Ligand binding to the porcine adipose tissue beta-adrenergic receptor.
We measured ligand binding to the beta-adrenergic receptor from porcine adipocytes using tritiated radioligands, dihydroalprenolol (DHA) and CGP-12177 (CGP), and an iodinated radioligand, cyanopindolol (ICP). Binding was measured in a crude plasma membrane preparation. Equilibrium saturation binding was regular for all three ligands; the Kd were approximately 4,000 pM for DHA, 600 pM for CGP, and 100 pM for ICP. Binding was stereospecific with each radioligand. Association of each radioligand was relatively rapid; dissociation was rapid and complete for DHA, initially rapid but ultimately incomplete for CGP, and minimal for ICP. The Kd estimated from kinetic data were approximately 1,000 pM for DHA and 100 pM for CGP. The receptor did not bind phentolamine, an alpha-adrenergic antagonist, except at concentrations greater than 10(-5) M. Propranolol was bound to the receptor with a Ki of approximately 8 nM regardless of the radioligand used. Metoprolol, a purported beta 1-adrenergic specific antagonist, was bound to the receptor with a Ki of approximately 300 nM when the radioligands were CGP or ICP but with a Ki of approximately 1,000 nM when the radioligand was DHA. The Ki for ICI 118,551, a purported beta 2-adrenergic specific antagonist, were approximately 500 nM when the radioligands were DHA or CGP but 125 nM when the radioligand was ICP. Thus, the choice of radioligand can influence the characterization of the beta-adrenergic receptor being studied.
Mersmann
H J
HJ
Department of Pediatrics, Baylor College of Medicine, Houston, TX 77030.
McNeel
R L
RL
D000273:Adipose Tissue / Q000378:metabolism*; D000319:Adrenergic beta-Antagonists / Q000737:chemistry / Q000378:metabolism*; D000818:Animals; D001667:Binding, Competitive; D004082:Dihydroalprenolol / Q000737:chemistry / Q000378:metabolism; D006160:Guanosine Triphosphate / Q000494:pharmacology; D007700:Kinetics; D008024:Ligands; D008968:Molecular Conformation; D010869:Pindolol / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D011412:Propanolamines / Q000737:chemistry / Q000378:metabolism*; D011943:Receptors, Adrenergic, beta / Q000378:metabolism*; D013552:Swine / Q000378:metabolism*; D013696:Temperature; D013997:Time Factors
D000319:Adrenergic beta-Antagonists; D008024:Ligands; D011412:Propanolamines; D011943:Receptors, Adrenergic, beta; C047975:cyanopindolol; D004082:Dihydroalprenolol; D006160:Guanosine Triphosphate; D010869:Pindolol; C033394:CGP 12177