Mapping of functional regions of Clostridium perfringens type A enterotoxin.
Studies were conducted to allow construction of an initial map of the structure-versus-function relationship of the Clostridium perfringens type A enterotoxin (CPE). Removal of the N-terminal 25 amino acids of CPE increased the primary cytotoxic effect of CPE but did not affect binding. CPE sequences required for at least four epitopes were also identified.
Hanna
P C
PC
Department of Molecular Genetics and Biochemistry, University of Pittsburgh School of Medicine, Pennsylvania 15261-2072.
Wieckowski
E U
EU
Mietzner
T A
TA
McClane
B A
BA
D000911:Antibodies, Monoclonal / Q000276:immunology; D003016:Clostridium perfringens / Q000378:metabolism*; D004768:Enterotoxins / Q000737:chemistry / Q000276:immunology / Q000633:toxicity*; D000939:Epitopes / Q000032:analysis; D010446:Peptide Fragments / Q000276:immunology / Q000633:toxicity; D013329:Structure-Activity Relationship
D000911:Antibodies, Monoclonal; D004768:Enterotoxins; D000939:Epitopes; D010446:Peptide Fragments; C035598:enterotoxin, Clostridium