Restriction endonuclease analysis of Aspergillus fumigatus DNA.
OBJECTIVE
To develop a genome based DNA fingerprinting system for Aspergillus fumigatus mould.


METHODS
DNA was extracted from 21 isolates obtained from eight patients with an aspergilloma. This was with a freeze-dried mycelial extract fragmented in liquid nitrogen. DNA was subsequently purified by phenol-chloroform extraction followed by ultracentrifugation on a caesium chloride gradient. The DNA was restricted by EcoRI and Xba I.


RESULTS
All isolates were identical when cut by EcoRI; Xba I delineated six DNA types.


CONCLUSIONS
DNA fingerprinting can be used to type isolates of A fumigatus. Strains from within an aspergilloma which were morphologically distinct could either have the identical DNA fingerprint or produce a unique type.
Department of Medical Microbiology, University of Manchester Medical School.
J P
JP
Burnie
A
A
Coke
R C
RC
Matthews
D001228:Aspergillosis / Q000382:microbiology*; D001232:Aspergillus fumigatus / Q000145:classification* / Q000235:genetics; D016172:DNA Fingerprinting; D004271:DNA, Fungal / Q000032:analysis*; D004587:Electrophoresis, Agar Gel; D016681:Genome, Fungal; D006801:Humans; D012150:Polymorphism, Restriction Fragment Length
D004271:DNA, Fungal