New pyrrolobenzodiazepine antibiotics, RK-1441A and B. I. Biological properties.
Streptomyces griseus and bacteriophage B were used for an assay system detecting anti-bacteriophage antibiotics. Streptomyces sp. RK-1441 was found to produce antibacteriophage antibiotics, RK-1441A and B, which are pyrrolo[1,4]benzodiazepine group antibiotics related to neothramycin. Both RK-1441A and B had antibacteriophage activity. The former showed antimicrobial activity on a hypersensitive strain of E. coli for antitumor antibiotics, but the later did not show the activity. Restriction enzyme assay suggested that RK-1441A formed adducts with guanine residues in DNA strands. RK-1441B was not active in vitro, but it might be converted to the active form in host organisms.
Antibiotics Laboratory, RIKEN (The Institute of Physical and Chemical Research), Saitama, Japan.
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Osada
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Ishinabe
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Yano
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Kajikawa
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Isono
D000900:Anti-Bacterial Agents / Q000096:biosynthesis / Q000378:metabolism / Q000494:pharmacology*; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology; D001435:Bacteriophages / Q000187:drug effects; D001570:Benzodiazepinones / Q000378:metabolism / Q000494:pharmacology; D002470:Cell Survival; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004279:DNA, Viral / Q000032:analysis / Q000378:metabolism; D005285:Fermentation; D006801:Humans; D007942:Leukemia, Experimental; D008826:Microbial Sensitivity Tests; D013302:Streptomyces / Q000145:classification / Q000378:metabolism*; D014407:Tumor Cells, Cultured / Q000187:drug effects
D000900:Anti-Bacterial Agents; D000903:Antibiotics, Antineoplastic; D001570:Benzodiazepinones; D004279:DNA, Viral; C069060:RK 1441A; C069061:RK 1441B; D004262:DNA Restriction Enzymes