Comparison of the University of Wisconsin preservation solution and other crystalloid perfusates in a 30-hour rabbit lung preservation model.
The University of Wisconsin solution, which contains a high potassium concentration (120 mmol/L), was evaluated for rabbit lung preservation by comparing it with a modified University of Wisconsin solution with low potassium (4 mmol/L), a low-potassium dextran solution (4 mmol/L), and simple surface cooling. In the first three groups rabbit lungs were flushed in situ with the solution (n = 5 in each group); then the lung-heart block was harvested and stored at 10 degrees C for 30 hours. In the surface cooling group the lungs were harvested without flushing and then simply immersed in saline and stored. For assessment, the stored lung was ventilated with room air and perfused with fresh venous blood at a rate of 40 ml/min for 10 minutes. Assessment of lung function included gas analysis of effluent blood, mean pulmonary artery perfusion pressure, and peak airway pressure. Among these parameters, oxygen tension was most sensitive. Oxygen tension at 10 minutes' perfusion in the modified University of Wisconsin (95 +/- 6 mm Hg) and low-potassium dextran (99 +/- 4 mm Hg) groups was significantly higher than that in the surface cooling (61 +/- 7 mm Hg) and University of Wisconsin (51 +/- 7 mm Hg) groups. There was no difference between the modified University of Wisconsin and low-potassium dextran groups or between the surface cooling and University of Wisconsin groups. We conclude that the low-potassium University of Wisconsin solution is superior to the high-potassium University of Wisconsin solution and that the lactobionate and raffinose included in the University of Wisconsin solution as impermeants do not improve lung preservation in this model.
Division of Cardiothoracic Surgery, Washington University School of Medicine, St. Louis, Mo.
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S
Miyoshi
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Shimokawa
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Schreinemakers
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Date
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Weder
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Harper
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JD
Cooper
D000241:Adenosine; D000493:Allopurinol; D000818:Animals; D003080:Cold Temperature; D000077324:Crystalloid Solutions; D003911:Dextrans / Q000494:pharmacology; D005069:Evaluation Studies as Topic; D005978:Glutathione; D007328:Insulin; D007552:Isotonic Solutions; D008168:Lung; D009926:Organ Preservation; D019188:Organ Preservation Solutions; D010952:Plasma Substitutes; D011188:Potassium / Q000494:pharmacology; D011817:Rabbits; D011887:Raffinose; D012965:Sodium Chloride; D012996:Solutions; D013997:Time Factors
D000077324:Crystalloid Solutions; D003911:Dextrans; D007328:Insulin; D007552:Isotonic Solutions; D019188:Organ Preservation Solutions; D010952:Plasma Substitutes; D012996:Solutions; C055206:University of Wisconsin-lactobionate solution; D012965:Sodium Chloride; D000493:Allopurinol; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose; D011188:Potassium