Formation of a transfer product from stevioside by the cultures of Actinomycete.
An Actinomycete, strain K-128, which was isolated from soil, formed a transfer product when the strain was cultured in a medium containing both stevioside and curdlan. The transfer product in culture broth was separated by DIAION HP-20 column and TOYOPEARL HW-40F column chromatographies. From structural studies, the transfer product was identified as C13-O-beta-6(2)-beta-glucosylsophorosyl-C19-O-beta-glucopyranosyl steviol. This product was the first to be obtained by a culture of an Actinomycete.
Kusakabe
I
I
Institute of Applied Biochemistry, University of Tsukuba, Ibaraki, Japan.
Watanabe
S
S
Morita
R
R
Terahara
M
M
Murakami
K
K
D000190:Actinomyces / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D002855:Chromatography, Thin Layer; D004224:Diterpenes; D045786:Diterpenes, Kaurane; D005947:Glucose / Q000378:metabolism; D005959:Glucosidases / Q000378:metabolism; D005960:Glucosides / Q000494:pharmacology*; D006868:Hydrolysis; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D016339:Spectrometry, Mass, Fast Atom Bombardment; D013549:Sweetening Agents / Q000494:pharmacology*; D013729:Terpenes / Q000494:pharmacology*
D004224:Diterpenes; D045786:Diterpenes, Kaurane; D005960:Glucosides; D013549:Sweetening Agents; D013729:Terpenes; C012043:stevioside; D005959:Glucosidases; D005947:Glucose