[Detection of the HIV p24 antigen on lymphocyte membranes using flow cytometry].
The evaluation of the presence of p24 antigen on the membrane of peripheral blood mononuclear cells from 31 HIV infected individuals is presented. The study was performed by indirect immunofluorescence and flow cytometry and the data were analyzed by the Kolmogorov-Smirnov test. Values obtained [D/s(n)] result from the comparison of the fluorescence histograms of each sample with a control one. Cases showing p24 Ag on peripheral blood mononuclear cells also presented percentages of CD3, HLA-DR positive cells significantly higher than p24 negative ones. In addition, D/s(n) values were superior in symptomatic patients than in asymptomatic ones, which indicate the existence of a correlation between flow cytometry results, viral replication and clinical course. Nevertheless in this study, as well and in previous ones, a high degree of cross reactivity between the anti-p24 monoclonal antibody employed and normal lymphocytes has been observed. This reactivity is localized preferentially in the CD4 positive subset.
Departamento de Microbiología e Inmunología, Facultad de Medicina, Universidad del País Vasco, Bilbao.
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Madariaga
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Suárez
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Antón
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Campelo
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Lardelli
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Cisterna
D000911:Antibodies, Monoclonal / Q000276:immunology; D017252:CD3 Complex / Q000032:analysis; D016516:CD4-CD8 Ratio; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D003429:Cross Reactions; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D015483:HIV Antibodies / Q000276:immunology; D016655:HIV Core Protein p24 / Q000097:blood*; D006679:HIV Seropositivity / Q000097:blood* / Q000382:microbiology; D015497:HIV-1 / Q000302:isolation & purification*; D006684:HLA-DR Antigens / Q000032:analysis; D006801:Humans; D008214:Lymphocytes / Q000737:chemistry / Q000382:microbiology*
D000911:Antibodies, Monoclonal; D017252:CD3 Complex; D015483:HIV Antibodies; D016655:HIV Core Protein p24; D006684:HLA-DR Antigens
Detección de antígeno p24 del VIH en las membranas linfocitarias por citometría de flujo.