Recombinant brewer's yeast strains suitable for accelerated brewing.
Four brewer's yeast strains carrying the alpha-ald gene of Klebsiella terrigena (ex. Aerobacter aerogenes) or of Enterobacter aerogenes on autonomously replicating plasmids were constructed. The alpha-ald genes were linked either to the ADC1 promoter or to the PGK1 promoter of yeast Saccharomyces cerevisiae. In pilot scale brewing (50 l) with three of these recombinant yeasts the formation of diacetyl in beer was so low during fermentation that lagering was not required. All other brewing properties of the strains were unaffected and the quality of finished beers was as good as that of finished beer prepared with the control strain. The total process time of beer production could therefore be reduced to 2 weeks, in contrast to about 5 weeks required in the conventional process.
VTT, Biotechnical Laboratory, Espoo, Finland.
M L
ML
Suihko
K
K
Blomqvist
M
M
Penttilä
R
R
Gisler
J
J
Knowles
D001483:Base Sequence; D001515:Beer; D002262:Carboxy-Lyases / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D004755:Enterobacteriaceae / Q000235:genetics / Q000378:metabolism*; D005285:Fermentation; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D016296:Mutagenesis; D010957:Plasmids; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*
D002262:Carboxy-Lyases; C023746:acetolactate decarboxylase