An easy-to-handle semi-automated method for media development using a colorimetric viability assay and fractional factorial designs.
A rapid and effective semi-automated screening method has been developed for the development of growth media for mammalian cell culture. The method has proven to be a powerful tool for preliminary evaluation and comparison of new media formulations, but has some inherent disadvantages, which are important to recognise in order to interpret the results.
Rexen
P
P
Department of Biotechnology, Technical University of Denmark, Lyngby.
Kierulff
J V
JV
Emborg
C
C
D000818:Animals; D001331:Automation; D016466:CHO Cells; D002452:Cell Count; D002470:Cell Survival; D002478:Cells, Cultured; D003124:Colorimetry; D006224:Cricetinae; D003470:Culture Media; D006863:Hydrogen-Ion Concentration; D051379:Mice; D013778:Tetrazolium Salts; D013844:Thiazoles
D003470:Culture Media; D013778:Tetrazolium Salts; D013844:Thiazoles; C022616:thiazolyl blue