Specific inhibition of mRNA accumulation for lymphokines in human T cell line Jurkat by mycobacterial lipoarabinomannan antigen.
The immunomodulatory effect of Mycobacterium tuberculosis-derived lipoarabinomannan (LAM) on mitogen/antigen-induced expression of mRNAs for a number of cytokines in human monocytic cell line Mono-Mac-6 and in T cell line Jurkat was investigated. Interestingly, LAM exhibited a down-regulatory effect on the accumulation of mRNAs for IL-2, IL-3, granulocyte-macrophage colony-stimulating factor (GM-CSF), and IL-2 receptor alpha (IL-2R alpha) in T cells co-stimulated with phytohaemagglutinin-P (PHA) and 4 beta-phorbol-12-myristyl-13-acetate (PMA). In human Mono-Mac-6 cells. LAM has a weak inhibitory effect on the lipopolysaccharide (LPS)-induced mRNA accumulation for IL-1 beta, a slight stimulatory effect on mRNAs accumulation for IL-8 and tumour necrosis factor-alpha (TNF-alpha), but clearly no effect on mRNA accumulation for intercellular adhesion molecule-1 (ICAM-1). These findings imply that LAM may contribute to the immunologic defects associated with a number of mycobacterial infections by modulating these mediators.
Sandoz Forschungsinstitut, Vienna, Austria.
C S
CS
Chujor
B
B
Kuhn
B
B
Schwerer
H
H
Bernheimer
W R
WR
Levis
D
D
Bevec
D000942:Antigens, Bacterial / Q000276:immunology*; D001483:Base Sequence; D015152:Blotting, Northern; D002460:Cell Line; D002478:Cells, Cultured; D016207:Cytokines / Q000032:analysis; D006801:Humans; D008070:Lipopolysaccharides / Q000276:immunology*; D008222:Lymphokines / Q000032:analysis*; D008969:Molecular Sequence Data; D009000:Monocytes / Q000276:immunology; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D015345:Oligonucleotide Probes; D012313:RNA / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis*; D013601:T-Lymphocytes / Q000276:immunology*
D000942:Antigens, Bacterial; D016207:Cytokines; D008070:Lipopolysaccharides; D008222:Lymphokines; D015345:Oligonucleotide Probes; D012333:RNA, Messenger; C050016:lipoarabinomannan; D012313:RNA