Interaction of cholesterol-conjugated alkylating oligonucleotide derivatives with cellular biopolymers.
Interactions of oligonucleotide derivatives with mammalian cells and cellular biopolymers have been investigated. The derivatives were oligonucleotides bearing an alkylating 2-chloroethylamino group at the 3'-end and a cholesterol residue at the 5'-terminal phosphate. These compounds are readily taken up by cells and react with cellular DNA, RNA and some proteins which may play a role in delivery of the compounds into cells.
Ryte
A S
AS
Institute of Bioorganic Chemistry, Siberian Division of the USSR Academy of Sciences, Novosibirsk.
Karamyshev
V N
VN
Nechaeva
M V
MV
Guskova
Z V
ZV
Ivanova
E M
EM
Zarytova
V F
VF
Vlassov
V V
VV
D000478:Alkylation; D001201:Ascites / Q000378:metabolism; D001345:Autoradiography; D001704:Biopolymers; D002460:Cell Line; D002784:Cholesterol / Q000378:metabolism*; D004247:DNA / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D009841:Oligonucleotides / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism; D012313:RNA / Q000378:metabolism; D014407:Tumor Cells, Cultured
D001704:Biopolymers; D009841:Oligonucleotides; D011506:Proteins; D012313:RNA; D004247:DNA; D002784:Cholesterol