Effect of inclusion body production on culture turbidity and cell dry weight in growing bacterial cultures.
An approach to the optimization of product yield in an inducible inclusion body-producing system is presented. Following induction by indoleacrylic acid (IAA) of a trpLE-HIVgp41 fusion protein, we found a large increase in culture turbidity and single-cell dry weight. After an initial transition phase, new and constant values for specific growth rate, single-cell light turbidity, and single cell dry weight were achieved, allowing for the determination of optimal conditions for product formation.
Hwang
S O
SO
Department of Chemical Engineering, Tufts University, Medford, Massachusetts 02155.
Feldberg
R S
RS
D002273:Carcinogens / Q000494:pharmacology; D002452:Cell Count; D002455:Cell Division; D004926:Escherichia coli / Q000235:genetics / Q000648:ultrastructure*; D015700:HIV Envelope Protein gp41 / Q000235:genetics; D002479:Inclusion Bodies; D007211:Indoles / Q000494:pharmacology; D009391:Nephelometry and Turbidimetry; D010957:Plasmids / Q000502:physiology; D011994:Recombinant Proteins / Q000096:biosynthesis
D002273:Carcinogens; D015700:HIV Envelope Protein gp41; D007211:Indoles; D011994:Recombinant Proteins; C001446:indoleacrylic acid