Immunological evaluation of harvested stem cells obtained by leukapheresis after chemotherapy.
Some patients suffering from malignancies may benefit of myeloablative chemotherapy followed by hematological reconstitution with autologous peripheral blood reinfusion. A quick evaluation of the number of hematopoietic progenitors present in leukapheresis blood samples is necessary to ensure the collection of a sufficient number of these cells. A study was performed on a series of 25 leukapheresis following initial chemotherapy. The number of granulomonocytic colony-forming unit (CFU-GM) and the number of CD34+ cells were evaluated simultaneously, in each sample. Results have shown a relatively strong linear correlation between both methods of evaluation of hematopoietic progenitors, suggesting that immunophenotyping could be a useful method to estimate the number of progenitors.
Research Department of Hematopoietic and Tumor Differenciation, School of Medicine, University of Rouen, Saint Etienne du Rouvray, France.
G
G
Teixeira
B
B
Lenormand
P
P
Jean
D
D
Fardoun
E
E
Sumereau-Dassin
D
D
Bastit
H
H
Tilly
H
H
Piguet
M
M
Monconduit
J P
JP
Vannier
D000328:Adult; D015703:Antigens, CD / Q000032:analysis*; D018952:Antigens, CD34; D000970:Antineoplastic Agents / Q000627:therapeutic use*; D002478:Cells, Cultured; D002675:Child, Preschool; D005434:Flow Cytometry; D006412:Hematopoietic Stem Cells / Q000276:immunology* / Q000473:pathology; D006801:Humans; D007937:Leukapheresis; D009369:Neoplasms / Q000097:blood* / Q000188:drug therapy; D012016:Reference Values
D015703:Antigens, CD; D018952:Antigens, CD34; D000970:Antineoplastic Agents