Nitric oxide stimulates the ADP-ribosylation of a 41-kDa cytosolic protein in Dictyostelium discoideum.
Nitric oxide-releasing compounds were shown to activate an ADP-ribosyltransferase activity in the cytosol of Dictyostelium discoideum. The enzyme ADP-ribosylated a cytosolic protein of approximately 41 kDa, p41. Neither cGMP nor GTP and its analogues affected this ADP-ribosylation. p41 differs from other substrates ADP-ribosylated by cholera, pertussis, or diphtheria toxins. Treatment of ADP-ribosylated p41 with snake venom phosphodiesterase released adenosine 5'-monophosphate, indicating a mono-ADP-ribose-protein linkage. This linkage was stable to neutral hydroxylamine but was sensitive to mercury ions and iodomethane, suggesting an attachment to a cysteine residue. Treatment of intact cells with nitric oxide-releasing compounds appeared to stimulate the ADP-ribosylation of p41 and this modification was reversible.
E.A. Doisy Department of Biochemistry and Molecular Biology, St. Louis University School of Medicine, MO 63104.
Y
Y
Tao
A
A
Howlett
C
C
Klein
D036002:ADP Ribose Transferases; D000246:Adenosine Diphosphate Ribose / Q000378:metabolism; D000818:Animals; D003545:Cysteine / Q000737:chemistry; D003600:Cytosol / Q000378:metabolism; D004023:Dictyostelium / Q000201:enzymology*; D004789:Enzyme Activation; D005656:Fungal Proteins / Q000737:chemistry / Q000378:metabolism*; D006162:Guanylate Cyclase / Q000378:metabolism; D008970:Molecular Weight; D009569:Nitric Oxide / Q000494:pharmacology*; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism*
D005656:Fungal Proteins; D000246:Adenosine Diphosphate Ribose; D009569:Nitric Oxide; D036002:ADP Ribose Transferases; D011065:Poly(ADP-ribose) Polymerases; D006162:Guanylate Cyclase; D003545:Cysteine