Detection of P-glycoprotein on endothelial and endocrine cells of the human pancreatic islets by C 494 monoclonal antibody.
P-glycoprotein, an integral membrane protein acting as an energy-dependent efflux pump, has been detected immunocytochemically in the human pancreatic islets using C 494 monoclonal antibody. Intense P-glycoprotein immunoreactivity was found in both endothelial cells of islet blood capillaries and in endocrine cells. Strong expression of P-glycoprotein has been found in the capillary blood vessels at blood-tissue barrier sites and in numerous kinds of cells with secretory/excretory function. Therefore the present findings suggest that P-glycoprotein may play a role in controlling the composition of the extracellular fluids and the intracellular milieu of endocrine islet cells and possibly in regulating their secretory activity.
Department of Human Anatomy and Histology, University of Florence, Italy.
D
D
Bani
M L
ML
Brandi
C A
CA
Axiotis
T
T
Bani-Sacchi
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D000328:Adult; D000911:Antibodies, Monoclonal; D004730:Endothelium, Vascular / Q000378:metabolism; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007515:Islets of Langerhans / Q000098:blood supply / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000032:analysis*
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D000911:Antibodies, Monoclonal; D008562:Membrane Glycoproteins