[Detection of estrogen receptor (ER) mRNA by use of reverse transcriptase-polymerase chain reaction (RT-PCR) assay; comparison with dextran coated charcoal (DCC) assay and immunocytochemical assay].
A new method for estrogen receptor (ER) mRNA was performed on 33 human breast tumors, using a reverse transcriptase-polymerase chain reaction (RT-PCR) assay by the method of Fuqua et al. In a preliminary experiment using the MCF-7 breast tumor cell line, ER/beta-actin ratio was almost same. ER protein was estimated by a dextran coated charcoal (DCC) assay and by an ER-immunocytochemical (ER-ICA) assay using a specific monoclonal antibody. We found RT-PCR assay correlates with ER-ICA assay (r = 0.664, p less than 0.01), whereas no significant correlation was seen between RT-PCR assay and DCC assay. These results suggests that RT-PCR assay is suitable for detection of ER from small amounts of tissue.
Ozaki
T
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Dept. of Surgery, Osaka University, Suita, Japan.
D015139:Blotting, Southern; D001943:Breast Neoplasms / Q000737:chemistry*; D002606:Charcoal; D003911:Dextrans; D004586:Electrophoresis; D006801:Humans; D007150:Immunohistochemistry; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis; D012194:RNA-Directed DNA Polymerase; D011869:Radioligand Assay; D011960:Receptors, Estrogen / Q000032:analysis*; D014407:Tumor Cells, Cultured
D003911:Dextrans; D012333:RNA, Messenger; D011960:Receptors, Estrogen; D002606:Charcoal; D012194:RNA-Directed DNA Polymerase