Purification and properties of mycodextranase from Streptomyces sp. J-13-3.
An enzyme hydrolyzing nigeran (alternating alpha-1,3- and alpha-1,4-linked glucan) was purified from the culture filtrate of Streptomyces sp. J-13-3, which lysed the cell wall of Aspergillus niger, by percipitation with ammonium sulfate and column chromatographies on DEAE-Sephadex A-50, CM-Sephadex C-50, chromatofocusing, and Sephadex G-100. The final preparation was homogenous in polyacrylamide gel electrophoresis (PAGE). The molecular weight of the enzyme was 68,000 by SDS-PAGE and gel filtration. The optimum pH and temperature for the enzyme activity were 6.0 and 50 degrees C, respectively. The enzyme was stable in the pH range from 6.0 to 8.0 and up to 50 degrees C. The enzyme activity was inhibited significantly by Hg+, Hg2+, and p-chloromercuribenzoic acid. The Km (mg/ml) for nigeran was 3.33. The enzyme specifically hydrolyzed nigeran into nigerose and nigeran tetrasaccharide by an endo-type of action, indicating it to be a mycodextranase (EC 3.2.1.61) that splits only the alpha-1,4-glucosidic linkages in nigeran.
Okazaki
K
K
Department of Bioresource Science, Faculty of Agriculture, Kagawa University, Japan.
Abe
T
T
Saruwatari
K
K
Kato
F
F
Maruyama
K
K
Tagawa
K
K
D002240:Carbohydrate Sequence; D004591:Electrophoresis, Polyacrylamide Gel; D004795:Enzyme Stability; D005656:Fungal Proteins / Q000096:biosynthesis / Q000302:isolation & purification* / Q000378:metabolism; D005936:Glucans / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000096:biosynthesis / Q000302:isolation & purification* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D008969:Molecular Sequence Data; D008970:Molecular Weight; D013302:Streptomyces / Q000201:enzymology*; D013379:Substrate Specificity
D005656:Fungal Proteins; D005936:Glucans; C001290:glucan nigeran; D006026:Glycoside Hydrolases; C021817:mycodextranase