Embedded adrenal cells graft reduced local and early nonspecific inflammatory phenomena which follow agarose beads implantation.
Microencapsulation of adrenal cells is proposed for reducing the nonspecific inflammatory reaction observed around polymer implants. This hypothesis was tested by comparing both host cellular reaction and the surrounding graft cell populations which appeared either when agarose embedded cells or when empty agarose beads were implanted. Our results showed that the fibrotic material that surrounded the implanted empty agarose microbeads was not as severe and important when adrenal cells were present. Similarly, T lymphocyte population surrounding the graft was considerably reduced together with the percentage of CD4 and CD8 positive cell subpopulations. The activation macrophage marker IaD disappeared. Our results support the hypothesis that embedded adrenal cells may be a suitable solution for reducing early inflammatory events due to microcapsules implantation.
INSERM U.306, Université de Bordeaux II.
C
C
Cadic
S
S
Vitiello
H
H
Gin
P J
PJ
Neveu
B
B
Dupuy
D000302:Adrenal Cortex / Q000166:cytology / Q000637:transplantation* / Q000648:ultrastructure; D000818:Animals; D002214:Capsules; D005434:Flow Cytometry; D006085:Graft Survival; D006099:Granuloma / Q000473:pathology / Q000517:prevention & control; D007249:Inflammation / Q000517:prevention & control*; D008214:Lymphocytes / Q000166:cytology / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008815:Mice, Inbred Strains; D008854:Microscopy, Electron; D012685:Sepharose
D002214:Capsules; D012685:Sepharose