Production of arachidonic acid by Mortierella alpina ATCC 32222.
When Mortierella alpina ATCC 32222 was incubated in a glucose salts medium at 25 degrees C the biomass (17.5 g/l) contained 9.62% arachidonic acid which amounted to 54% (w/w) of total biomass lipids. When the glucose concentration in the medium was varied from 0 to 150 g/l, the percentage of arachidonic acid in biomass and in lipids was highest at a glucose concentration of 30 g/l, but highest yield of arachidonic acid per litre of culture broth was observed at a glucose concentration of 100 g/l. While production of biomass reached a plateau of 17 g/l after a 3-day incubation at 25 degrees C, the percentage of arachidonic acid in lipids and biomass increased dramatically from 3 to 6 days with a concurrent arachidonic acid yield increase from 0.89 to 1.63 g/l. Optimum initial culture pH for arachidonic acid production was in the range 6.0-6.7. By increasing the concentration of the glucose salts medium three-fold, yields of biomass and arachidonic acid were increased to 35.8 g/l and 3.73 g/l, respectively.
Department of Biology, University of Waterloo, Ontario, Canada.
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Bajpai
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Bajpai
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Ward
D016718:Arachidonic Acid / Q000096:biosynthesis*; D002244:Carbon / Q000378:metabolism; D003470:Culture Media; D005227:Fatty Acids / Q000096:biosynthesis; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008055:Lipids / Q000096:biosynthesis; D009090:Mucorales / Q000254:growth & development / Q000378:metabolism*; D009584:Nitrogen / Q000378:metabolism; D013696:Temperature
D003470:Culture Media; D005227:Fatty Acids; D008055:Lipids; D016718:Arachidonic Acid; D002244:Carbon; D005947:Glucose; D009584:Nitrogen