Transcriptional activation of jun and actin genes by estrogen during mitogenic stimulation of rat uterine cells.
Estrogens induce transcriptional activation of c-fos and c-myc proto-oncogenes during mitogenic stimulation of human, chicken, mouse and rat cells in vivo and in vitro. In this paper we show that 17 beta-estradiol injected into adult ovariectomized rats increases c-jun, jun-B and jun-D gene transcription in the uterus. Kinetics and amplitude of response are different for each gene, since c-jun is activated first, within 30 min after injection, followed by jun-D and jun-B, 60 and 90 min after injection, respectively. Maximal activation of jun-B marks a drop in transcription of all the jun genes. Furthermore, transcriptional activation by 17 beta-estradiol of the growth-regulated beta- and gamma-cytoskeletal actin genes is prevented by an inhibitor of protein synthesis, indicating that it is a secondary response to the hormone. These data support the hypothesis that during growth stimulation of target cells the estrogen receptor induces transcription of regulatory genes, triggering in this way a cascade of gene regulation events that results in progression through the cell cycle.
Istituto di Patologia generale e Oncologia, Prima Facoltà di Medicina e Chirurgia, Università di Napoli, Italy.
L
L
Cicatiello
C
C
Ambrosino
B
B
Coletta
M
M
Scalona
V
V
Sica
F
F
Bresciani
A
A
Weisz
D000199:Actins / Q000235:genetics*; D000818:Animals; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism*; D015342:DNA Probes; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D016762:Genes, fos; D016758:Genes, jun / Q000187:drug effects; D016259:Genes, myc; D007700:Kinetics; D010052:Ovariectomy; D011061:Poly A / Q000235:genetics / Q000302:isolation & purification; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D014158:Transcription, Genetic / Q000187:drug effects; D014599:Uterus / Q000187:drug effects / Q000378:metabolism*
D000199:Actins; D015342:DNA Probes; D012333:RNA, Messenger; D011061:Poly A; D004958:Estradiol; D012313:RNA