Long term and large-scale cultivation of human hepatoma Hep G2 cells in hollow fiber bioreactor. Cultivation of human hepatoma Hep G2 in hollow fiber bioreactor.
Long-term and large scale cultivation of an anchorage-dependent cell line using an industrial scale hollow fiber perfusion bioreactor is described. Hep G2 cells (a human hepatoma cell line) were cultivated in an Acusyst-P (Endotronic) with a total fiber surface area of 7.2 m2 6 x 1.2m2) to produce Hep G2 crude conditioned medium (CCM). Pretreatment of the cellulose acetate hollow fibers with collagen enhances the attachment of the anchorage-dependent cells. We have succeeded in growing the Hep G2 cells in an antibiotics- and serum-free IMDM medium, supplemented with 50 micrograms/ml of Hep G2 CCM protein at inoculation. The Hep G2 cells replicate and secrete CCM protein in quantities comparable to those produced in DMEM containing 10% fetal calf serum (FCS). The highest CCM protein productivity during the 80-day cultivation was 1.1 g/day with a total of 30 g of protein accumulated. Hep G2 CCM (20-40 micrograms protein/ml) was comparable to or even better than 10% FCS in supporting the growth of Molt-4 (a human T leukemia cell line) and FO (a mouse myeloma cell line) cells in vitro. The availability of this large amount of Hep G2 CCM will aid the further purification and characterization of growth factor(s) which could be used as serum substituents.
Liu
J J
JJ
Development Center for Biotechnology, Taipei, Taiwan, ROC.
Chen
B S
BS
Tsai
T F
TF
Wu
Y J
YJ
Pang
V F
VF
Hsieh
A
A
Hsieh
J H
JH
Chang
T H
TH
D006528:Carcinoma, Hepatocellular; D002448:Cell Adhesion; D002455:Cell Division; D003094:Collagen; D003470:Culture Media; D006801:Humans; D007700:Kinetics; D008113:Liver Neoplasms; D008722:Methods; D013997:Time Factors; D014407:Tumor Cells, Cultured
D003470:Culture Media; D003094:Collagen