Isolation and characterization of a 2-(2,4-dichlorophenoxy) propionic acid-degrading soil bacterium.
The 2-(2,4-dichlorphenoxy)propionic acid (2,4-DP)-degrading bacterial strain MH was isolated after numerous subcultivations of a mixed culture obtained by soil-column enrichment and finally identified as Flavobacterium sp. Growth of this strain was supported by 2,4-DP (maximum specific growth rate 0.2 h-1) as well as by 2,4-dichlorophenoxyacetic acid (2,4-D), 4-(2,4-dichlorophenoxy)butyric acid (2,4-DB), and 2-(4-chloro-2-methylphenoxy)propionic acid (MCPP) as sole sources of carbon and energy under aerobic conditions. 2,4-DP-Grown cells (10(8] of strain MH degraded 2,4-dichlorophenoxyalkanoic acids, 2,4-dichlorophenol (2,4-DCP), and 4-chlorophenol at rates in the range of 30 nmol/h. Preliminary investigations indicate that cleavage of 2,4-DP results in 2,4-DCP, which is further mineralized via ortho-hydroxylation and ortho-cleavage of the resulting 3,5-dichlorocatechol.
Horvath
M
M
Abteilung für Mikrobielle Okologie und Methodik am Institut für Biochemische Technologie und Mikrobiologie, Technische Universität Wien, Vienna, Austria.
Ditzelmüller
G
G
Loidl
M
M
Streichsbier
F
F
D015084:2,4-Dichlorophenoxyacetic Acid / Q000031:analogs & derivatives* / Q000378:metabolism; D001673:Biodegradation, Environmental; D005417:Flavobacterium / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism; D007700:Kinetics; D012988:Soil Microbiology
D015084:2,4-Dichlorophenoxyacetic Acid; C010571:dichlorprop