Analysis of the gene polymorphism of ABO blood group specific transferases helps diagnosis of acquired B status.
Blood group typing of an aged patient suffering from ileus provided evidence for an acquired B. As a parameter independent of cell membrane molecules or secreted blood group substances, the nucleotide sequence polymorphism of A and B transferases was investigated. Restriction fragment length polymorphism of DNA chains amplified in a polymerase chain reaction from the coding region of the glycosyltransferase indicated that no gene for B transferase was present in the patient's genome. We conclude that the assessment of polymorphism of AB0 blood group transferase can be used as a marker independent of blood group molecules for confirming a suspected acquired B.
Institut für Blutgruppenserologie, Universität Wien (National Blood Group Reference Laboratory-WHO, Vienna, Austria.
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Fischer
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Faé
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Dub
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Pickl
D000017:ABO Blood-Group System / Q000235:genetics*; D000368:Aged; D000369:Aged, 80 and over; D001788:Blood Grouping and Crossmatching; D005260:Female; D005700:Galactosyltransferases / Q000172:deficiency / Q000235:genetics*; D006388:Hemagglutinins / Q000276:immunology*; D006801:Humans; D007415:Intestinal Obstruction / Q000209:etiology; D007518:Isoantibodies / Q000276:immunology*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012004:Rectal Neoplasms / Q000097:blood / Q000150:complications
D000017:ABO Blood-Group System; D006388:Hemagglutinins; D007518:Isoantibodies; D005700:Galactosyltransferases; C023155:blood-group-substance alpha-D-galactosyltransferase