Partial hydatidiform moles have impaired differentiated function (human chorionic gonadotropin and human placental lactogen secretion) in response to epidermal growth factor and 8-bromo-cyclic adenosine monophosphate.
OBJECTIVE
The null hypothesis is that partial hydatidiform moles have normal differentiated function (human chorionic gonadotropin and human placental lactogen secretion) in response to epidermal growth factor and 8-bromo-cyclic adenosine monophosphate.


METHODS
Two complete moles, 10 partial hydatidiform moles, and 19 normal first-trimester placentas in monolayer culture were exposed to 10 ng/ml epidermal growth factor, 1 mmol/L 8-bromo-cyclic adenosine monophosphate plus 1 mmol/L theophylline, or control. Human chorionic gonadotropin and human placental lactogen secretion was measured. Frequency of response to stimuli was compared by chi 2 analysis, and hormone secretion was compared by analysis of variance.


RESULTS
Partial moles demonstrated reduced frequencies of response of human chorionic gonadotropin and human placental lactogen to epidermal growth factor (partial moles 2/8 and 2/8, respectively; normal placentas 16/19 and 7/18, respectively; p less than 0.025) and of human chorionic gonadotropin to 8-bromo-cyclic adenosine monophosphate (partial moles 3/5, normal placentas 13/16; p less than 0.005).


CONCLUSIONS
Partial hydatidiform moles demonstrate impaired human chorionic gonadotropin and human placental lactogen secretory responsiveness to epidermal growth factor and cyclic nucleotides in comparison with normal first-trimester trophoblast.
Morrish
D W
DW
Department of Medicine, University of Alberta, Edmonton, Canada.
Honoré
L H
LH
Bhardwaj
D
D
D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000494:pharmacology*; D002135:Calcium-Binding Proteins / Q000032:analysis; D006063:Chorionic Gonadotropin / Q000378:metabolism*; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D005260:Female; D006801:Humans; D006828:Hydatidiform Mole / Q000737:chemistry / Q000378:metabolism*; D007124:Immunoenzyme Techniques; D007633:Keratins / Q000032:analysis; D010928:Placental Lactogen / Q000378:metabolism*; D011247:Pregnancy; D014407:Tumor Cells, Cultured; D014594:Uterine Neoplasms / Q000737:chemistry / Q000378:metabolism*; D014746:Vimentin / Q000032:analysis
D002135:Calcium-Binding Proteins; D006063:Chorionic Gonadotropin; D014746:Vimentin; C034733:oncomodulin; D015124:8-Bromo Cyclic Adenosine Monophosphate; D004815:Epidermal Growth Factor; D007633:Keratins; D010928:Placental Lactogen