Dopaminergic neurons in the cat dorsal motor nucleus of the vagus, demonstrated by dopamine, AADC and TH immunohistochemistry.
In the rostral part of the dorsal motor nucleus of the vagus of the cat, neurons do not contain histochemically detectable catecholamines, even though many perikarya contain both intense aromatic L-amino acid decarboxylase (AADC) immunoreactivity and strong monoamine oxidase enzymatic activity. Similarly located perikarya have distinct immunoreactivities to tyrosine hydroxylase (TH) and dopamine after treatment with colchicine. Since inhibition of monoamine oxidase fails to reveal dopamine in these cells, its absence in non-colchicine-treated animals cannot be due to rapid deamination. It appears that dopamine is synthesized by TH and AADC in dorsal motor vagal cells and is then rapidly transported from the perikarya.
Département de Médecine Expérimentale, CNRS UA1195, INSERM U52, Faculté de Médecine, Lyon, France.
K
K
Kitahama
C
C
Buda
J P
JP
Sastre
I
I
Nagatsu
B
B
Raynaud
M
M
Jouvet
M
M
Geffard
D000818:Animals; D001142:Aromatic-L-Amino-Acid Decarboxylases / Q000737:chemistry*; D002395:Catecholamines / Q000378:metabolism; D002415:Cats; D003078:Colchicine / Q000494:pharmacology; D004298:Dopamine / Q000737:chemistry / Q000502:physiology*; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D009474:Neurons / Q000502:physiology*; D014446:Tyrosine 3-Monooxygenase / Q000737:chemistry*; D014630:Vagus Nerve / Q000166:cytology* / Q000201:enzymology / Q000378:metabolism
D002395:Catecholamines; D014446:Tyrosine 3-Monooxygenase; D001142:Aromatic-L-Amino-Acid Decarboxylases; D003078:Colchicine; D004298:Dopamine