Identification of double-stranded RNA-binding domains in the interferon-induced double-stranded RNA-activated p68 kinase.
The double-stranded RNA (dsRNA)-binding domain of the human p68 kinase has been localized to the N-terminal half of the enzyme by using progressive deletion analysis and in vitro binding assays. To further define the domains responsible for binding to dsRNA, we cloned the mouse dsRNA-activated p65 kinase and used sequence alignment to identify conserved domains in the N-terminal region. Deletions in either of two 12-amino-acid-long and arginine- or lysine-rich regions abrogated binding to dsRNA. Moreover, in an in vivo growth inhibition assay in the yeast Saccharomyces cerevisiae, these mutants failed to exhibit a slow-growth phenotype.
Research Institute, Hospital for Sick Children, Toronto, Canada.
G S
GS
Feng
K
K
Chong
A
A
Kumar
B R
BR
Williams
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002460:Cell Line; D002872:Chromosome Deletion; D003001:Cloning, Molecular; D004789:Enzyme Activation; D004790:Enzyme Induction; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D011070:Poly I-C / Q000378:metabolism; D014176:Protein Biosynthesis; D011494:Protein Kinases / Q000235:genetics* / Q000378:metabolism*; D012330:RNA, Double-Stranded / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics / Q000254:growth & development; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic; D019892:eIF-2 Kinase
D012330:RNA, Double-Stranded; D011494:Protein Kinases; D019892:eIF-2 Kinase; D011070:Poly I-C