Differential expression of two msh-related homeobox genes Chox-7 and Chox-8 during chick limb development.
We have isolated two closely related cDNAs, Chox-7 and Chox-8, encoding homeodomain-containing proteins homologous to Drosophila msh. The Chox-7 and Chox-8 genes are chicken cognates of mouse Hox-7.1 and Hox-8.1, respectively. In situ hybridization using 3' regions of the cDNAs as probes revealed that the Chox-7 gene is highly expressed in the mesenchyme subjacent to the apical ectodermal ridge whereas Chox-8 expression is localized in the anterodistal mesenchymal region at early stages of limb formation, suggesting different roles during limb development. At later stages, both genes are expressed in the anterior and posterior mesenchymes and in the interdigital mesenchyme where programmed cell death occurs.
Department of Pharmacology, Kawasaki Medical School, Japan.
T
T
Nohno
S
S
Noji
E
E
Koyama
K
K
Nishikawa
F
F
Myokai
T
T
Saito
S
S
Taniguchi
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002642:Chick Embryo; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D015342:DNA Probes; D005121:Extremities / Q000196:embryology; D005786:Gene Expression Regulation; D015723:Gene Library; D005801:Genes, Homeobox; D051379:Mice; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D016133:Polymerase Chain Reaction; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
D015342:DNA Probes; D009838:Oligodeoxyribonucleotides; D004247:DNA