Analysis of a peptidoglycan hydrolase gene from Staphylococcus aureus NCTC 8325.
We have investigated the expression of the peptidoglycan hydrolase gene (lytA) of Staphylococcus aureus NCTC 8325. Results from in vitro transcription-translation analysis, maxicell experiments, and Northern (RNA) blot analysis suggest that the lytA gene encodes a polypeptide of M(r) approximately 50,000. Physical mapping data indicate that the lytA gene originated from prophage 11 in the NCTC 8325 strain.
Wang
X
X
Department of Biological Sciences, Illinois State University, Normal 61761.
Mani
N
N
Pattee
P A
PA
Wilkinson
B J
BJ
Jayaswal
R K
RK
D002874:Chromosome Mapping; D004252:DNA Mutational Analysis; D009238:N-Acetylmuramoyl-L-alanine Amidase / Q000235:genetics*; D011994:Recombinant Proteins / Q000096:biosynthesis; D013211:Staphylococcus aureus / Q000201:enzymology / Q000235:genetics*
D011994:Recombinant Proteins; D009238:N-Acetylmuramoyl-L-alanine Amidase