Strain improvement of Penicillium chrysogenum by recombinant DNA techniques.
The penDE gene from Penicillium chrysogenum has been isolated; the gene is located in close vicinity of the pcbC gene. Amplification of the pcbC-penDE gene cluster in Penicillium chrysogenum Wis54-1255 leads to a significant increase in penicillin production. In selected transformants an increase of up to 40% is observed.
Gist-brocades Research and Development, Delft, The Netherlands.
A E
AE
Veenstra
P
P
van Solingen
R A
RA
Bovenberg
L H
LH
van der Voort
D000217:Acyltransferases / Q000096:biosynthesis* / Q000235:genetics; D004352:Drug Resistance, Microbial; D004790:Enzyme Induction; D005656:Fungal Proteins / Q000096:biosynthesis* / Q000235:genetics; D005784:Gene Amplification; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D007438:Introns; D010404:Penicillin V / Q000378:metabolism*; D046915:Penicillin-Binding Proteins; D010408:Penicillium chrysogenum / Q000235:genetics* / Q000378:metabolism; D010692:Phleomycins / Q000494:pharmacology; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis* / Q000235:genetics; D012641:Selection, Genetic
D005656:Fungal Proteins; D046915:Penicillin-Binding Proteins; D010692:Phleomycins; D011993:Recombinant Fusion Proteins; D000217:Acyltransferases; C018756:acyl-CoA-6-aminopenicillanic acid acyltransferase; D010404:Penicillin V