Generation of 19 STS markers that can be anchored at specific sites on human chromosome 21.
Sequence-tagged sites (STSs) are short stretches of DNA that can be specifically detected by the polymerase chain reaction (PCR) and can be used to construct long-range physical maps of chromosomal DNA. These STSs can be detected by PCR assays developed by reference to data obtained from the sequencing of restriction fragment length polymorphism-DNA markers for chromosome 21, which were derived from recombinant lamba-phage and plasmid clones made from DNA of a human-hamster hybrid cell line. In this report, we describe the generation of 19 new STSs that are specific for human chromosome 21.
Tang
X
X
Gene Bank, Tsukuba Life Science Center, RIKEN, Institute of Physical and Chemical Research, Ibaraki, Japan.
Tashiro
H
H
Eki
T
T
Murakami
Y
Y
Soeda
E
E
Sakakura
T
T
Watkins
P C
PC
Yokoyama
K
K
D001483:Base Sequence; D002874:Chromosome Mapping; D002891:Chromosomes, Human, Pair 21; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D006822:Hybrid Cells; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D016324:Sequence Tagged Sites