Expression of chimeric human transferrin genes in transfected human tumor cell lines.
The iron-binding plasma protein transferrin (TF) is essential for supplying iron to cells and the prevention of iron toxicity. Our laboratory has cloned and characterized the human TF gene. Comparison of promoter regions of TF genes from human, chicken, and mouse reveals a strong nucleotide sequence conservation. This study demonstrates that 5' flanking regions of the TF gene are sufficient for directing expression of a heterologous gene in transgenic mice and transfected cells. For cell-specific expression, more than 150 base pairs appear to be required.
Department of Cellular & Structural Biology, University of Texas Health Science Center, San Antonio 78284.
G S
GS
Adrian
K
K
Fischbach
Y
Y
Lu
O
O
Gayet
E
E
Rivera
B H
BH
Bowman
D000818:Animals; D001483:Base Sequence; D002645:Chickens; D002678:Chimera / Q000235:genetics*; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D003001:Cloning, Molecular; D004247:DNA; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic; D012045:Regulatory Sequences, Nucleic Acid; D014162:Transfection / Q000235:genetics; D014168:Transferrin / Q000096:biosynthesis / Q000235:genetics*; D014407:Tumor Cells, Cultured
D014168:Transferrin; D004247:DNA; D015500:Chloramphenicol O-Acetyltransferase