Piperine-mediated changes in the permeability of rat intestinal epithelial cells. The status of gamma-glutamyl transpeptidase activity, uptake of amino acids and lipid peroxidation.
The effect of piperine (1-[5-(1,3-benzodioxol-5-yl)-1-oxo-2,4-pentadienyl]piperidin e), (from Piper nigrum) on the absorptive function of the intestine was studied. In vitro experiments showed that piperine (25-100 microM) significantly stimulated gamma-glutamyl transpeptidase (gamma-GT, EC 2.3.2.2.) activity, enhanced the uptake of radiolabelled L-leucine, L-isoleucine and L-valine, and increased lipid peroxidation in freshly isolated epithelial cells of rat jejunum. The kinetic behaviour of gamma-GT towards substrate and acceptor altered in the presence of piperine. In the presence of benzyl alcohol, an enhanced gamma-GT activity due to piperine was maintained. These results suggested that piperine may interact with the lipid environment to produce effects which lead to increased permeability of the intestinal cells.
Johri
R K
RK
Regional Research Laboratory (CSIR), Jammu-Tawi, India.
Thusu
N
N
Khajuria
A
A
Zutshi
U
U
D000470:Alkaloids; D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D052117:Benzodioxoles; D019905:Benzyl Alcohol; D001592:Benzyl Alcohols; D002463:Cell Membrane Permeability / Q000187:drug effects*; D004789:Enzyme Activation / Q000187:drug effects; D004848:Epithelium / Q000187:drug effects; D007422:Intestines / Q000187:drug effects*; D007583:Jejunum / Q000187:drug effects; D007700:Kinetics; D015227:Lipid Peroxidation / Q000187:drug effects*; D010880:Piperidines / Q000494:pharmacology*; D011092:Polyethylene Glycols; D053284:Polyunsaturated Alkamides; D051381:Rats; D005723:gamma-Glutamyltransferase / Q000378:metabolism*
D000470:Alkaloids; D000596:Amino Acids; D052117:Benzodioxoles; D001592:Benzyl Alcohols; D010880:Piperidines; D053284:Polyunsaturated Alkamides; D011092:Polyethylene Glycols; D005723:gamma-Glutamyltransferase; D019905:Benzyl Alcohol; C008922:piperine