Effects of dexamethasone on the growth and epidermal growth factor receptor expression of the OVCA 433 ovarian cancer cells.
We studied the correlation between dexamethasone (Dex) induced growth effects and modulation of epidermal growth factor receptor (EGFR) expression in OVCA 433 ovarian cancer cells. These cells express specific high and low affinity 125I-EGF binding sites and are growth stimulated by EGF. Dex exhibits mitoinhibitory effects by recruiting OVCA 433 cells in the G0-G1 phase of the cycle, but increases the number of both the high and the low affinity EGFR in a dose dependent manner. The maximal EGFR expression increase occurs after 24 h of Dex treatment consistently with Northern blot studies. The mitogenic activity of EGF in OVCA 433 cells is not affected by the presence of Dex. Moreover Dex growth inhibition occurs in JA1 cells, an ovarian cancer cell line which expresses unfunctional EGFR and which is unresponsive to EGF. Our results indicate that the Dex induced growth effects occur independently of EGFR expression.
Ferrandina
G
G
Department of Obstetrics and Gynecology, Catholic University, Rome, Italy.
Scambia
G
G
Benedetti Panici
P
P
Bonanno
G
G
De Vincenzo
R
R
Rumi
C
C
Bussa
S
S
Genuardi
M
M
Romano Spica
V
V
Spica Romano
V
V
Mancuso
S
S
D000911:Antibodies, Monoclonal / Q000276:immunology; D001665:Binding Sites; D015152:Blotting, Northern; D002455:Cell Division / Q000187:drug effects; D003907:Dexamethasone / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D015536:Down-Regulation; D004815:Epidermal Growth Factor / Q000378:metabolism* / Q000494:pharmacology; D066246:ErbB Receptors / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D005260:Female; D006801:Humans; D007399:Interphase / Q000187:drug effects; D010051:Ovarian Neoplasms / Q000378:metabolism* / Q000473:pathology; D012313:RNA / Q000032:analysis / Q000235:genetics; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D004815:Epidermal Growth Factor; D012313:RNA; D003907:Dexamethasone; D066246:ErbB Receptors