Chloroquine inhibits the insulin binding and the imprinting of nuclear envelope in Tetrahymena.
Chloroquine possessing lysosomotrop effects inhibits the development of insulin imprinting both on the plasma membrane and the nuclear envelope. Simultaneously chloroquine can inhibit the insulin binding in the nucleus itself but not in the plasma membrane. There is a dose dependent increase of binding of labelled insulin to the nuclear envelope. The control related binding of nuclei pretreated with insulin or chloroquine is similar and have a direct ratio, independently of the applied concentrations.
Department of Biology, Semmelweis University Medical School, Budapest, Hungary.
H
H
Hegyesi
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P
Kovács
G
G
Csaba
D000818:Animals; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D002738:Chloroquine / Q000494:pharmacology*; D007328:Insulin / Q000378:metabolism* / Q000494:pharmacology; D009685:Nuclear Envelope / Q000187:drug effects / Q000378:metabolism; D011972:Receptor, Insulin / Q000187:drug effects / Q000378:metabolism; D013769:Tetrahymena pyriformis / Q000187:drug effects* / Q000378:metabolism*
D007328:Insulin; D002738:Chloroquine; D011972:Receptor, Insulin