A new method for the encapsulation of mammalian cells.
A new encapsulation method was developed for the cultivation of mammalian cells. The capsules were produced using a solution of sodium cellulose sulphate (CS)(1.5%) and poly-dimethyl-diallyl-ammonium chloride (PDMDAAC). When CS droplets fell into the precipitation bath consisting of a 2% solution of PDMDAAC, immediately a membrane at the interphase was built up. The influences of varying encapsulation process parameters on capsule characteristics, cell growth, and monoclonal antibody production were tested. This new method showed advantages when compared to other methods mainly due to time simplicity of the whole process.
Merten
O W
OW
Institut Pasteur, Technologie Cellulaire, Paris, France.
Dautzenberg
H
H
Palfi
G E
GE
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000096:biosynthesis; D002214:Capsules; D002460:Cell Line; D002482:Cellulose / Q000031:analogs & derivatives; D046508:Culture Techniques / Q000379:methods; D006825:Hybridomas / Q000276:immunology / Q000502:physiology*; D007074:Immunoglobulin G / Q000032:analysis / Q000096:biosynthesis; D051379:Mice; D011095:Polyethylenes; D011108:Polymers; D000644:Quaternary Ammonium Compounds
D000911:Antibodies, Monoclonal; D002214:Capsules; D007074:Immunoglobulin G; D011095:Polyethylenes; D011108:Polymers; D000644:Quaternary Ammonium Compounds; C041004:poly-N,N-dimethyl-N,N-diallylammonium chloride; D002482:Cellulose; C020254:cellulose sulfate