Metabolic roles of peptone and yeast extract for the culture of a recombinant strain of Escherichia coli.
The influence of complex compounds on the growth of a recombinant strain of Escherichia coli containing the gene encoding glyceraldehyde 3-phosphate dehydrogenase, as well as the production of this enzyme have been studied. Batchwise cultures led to an accumulation of acetate, which was not utilized in a yeast extract-free medium. After glucose exhaustion, growth stopped and enzyme activity decreased. Whereas yeast extract allowed acetate assimilation and growth, peptone stabilized the enzymatic activity. The addition of both compounds resulted in optimal performances for enzyme production.
CNRS-ENSAIA, Vandoeuvre-les-Nancy, France.
N
N
Nancib
C
C
Branlant
J
J
Boudrant
D003470:Culture Media; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics / Q000254:growth & development*; D005285:Fermentation; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000096:biosynthesis / Q000235:genetics; D010446:Peptide Fragments / Q000096:biosynthesis / Q000235:genetics; D010461:Peptones / Q000378:metabolism*; D015002:Yeast, Dried / Q000378:metabolism*
D003470:Culture Media; D010446:Peptide Fragments; D010461:Peptones; C068142:glyceraldehyde 3-phosphate dehydrogenase (304-313); D005987:Glyceraldehyde-3-Phosphate Dehydrogenases