A highly sensitive enzyme-linked immunosorbent assay for Clostridium perfringens enterotoxin.
A highly sensitive enzyme-linked immunosorbent assay (ELISA) for quantitation of Clostridium perfringens enterotoxin (CPE) by a sandwich method with polystyrene beads was elaborated. The ELISA was very sensitive with a detection limit of 1 pg/ml of CPE. Clostridium perfringens culture fluid did not interfere with the assay. This ELISA may be useful for the mass screening for Cl. perfringens producing small amounts of CPE.
Uemura
T
T
Department of Veterinary Medicine, College of Agriculture, University of Osaka Prefecture, Japan.
Yoshitake
S
S
Hu
D
D
Kajikawa
T
T
D001709:Biotechnology; D003015:Clostridium Infections / Q000209:etiology / Q000382:microbiology; D003016:Clostridium perfringens / Q000472:pathogenicity; D004768:Enterotoxins / Q000032:analysis* / Q000592:standards; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods* / Q000592:standards / Q000706:statistics & numerical data; D005517:Foodborne Diseases / Q000209:etiology / Q000382:microbiology; D006801:Humans; D012015:Reference Standards; D012680:Sensitivity and Specificity
D004768:Enterotoxins; C035598:enterotoxin, Clostridium