Alpha 1-6(alpha 1-3)-difucosylation of the asparagine-bound N-acetylglucosamine in honeybee venom phospholipase A2.
Chymotryptic glycopeptides were prepared from a honeybee (Apis mellifica) venom phospholipase A2 (E.C. 3.1.1.4) fraction, with high affinity towards lentil (Lens culinaris) lectin. Treatment of the glycopeptide mixture with peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase A, followed by HPLC fractionation, yielded two oligosaccharides, which were analysed by 500 MHz 1H-NMR spectroscopy to give the following structures [formula: see text] This is the first report on a naturally occurring glycoprotein N-glycan with two fucose residues linked to the asparagine-bound N-acetylglucosamine.
Institut für Chemie, Universität für Bodenkultur, Vienna, Austria.
E
E
Staudacher
F
F
Altmann
L
L
März
K
K
Hård
J P
JP
Kamerling
J F
JF
Vliegenthart
D000117:Acetylglucosamine / Q000737:chemistry*; D001216:Asparagine / Q000737:chemistry*; D001514:Bee Venoms / Q000201:enzymology*; D002240:Carbohydrate Sequence; D005643:Fucose / Q000032:analysis* / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000032:analysis* / Q000737:chemistry; D010741:Phospholipases A / Q000737:chemistry*; D054467:Phospholipases A2
D001514:Bee Venoms; D009844:Oligosaccharides; D005643:Fucose; D001216:Asparagine; D010741:Phospholipases A; D054467:Phospholipases A2; D000117:Acetylglucosamine