The acu-1 gene of Coprinus cinereus is a regulatory gene required for induction of acetate utilisation enzymes.
We have isolated a gene from Coprinus cinereus which cross-hybridises to the facA and acu-5 genes of Aspergillus nidulans and Neurospora crassa, respectively. These genes encode acetyl-CoA synthetase, an enzyme which is inducible by acetate and required for growth on acetate as sole carbon source. We have designated the C. cinereus gene acs-1 and have used transformation to demonstrate its functional homology to the ascomycete genes by complementation of an N. crassa acu-5 mutation. The acs-1 gene has never been identified by mutation; mutations leading to loss of acetyl-CoA synthetase function map to another gene, acu-1. Using Northern analyses we have shown that acu-1 has a regulatory function that is required for acetate-induced transcription of acs-1 and of another acetate utilisation gene, acu-7, the isocitrate lyase structural gene.
School of Biological Sciences, Queen Mary and Westfield College, University of London, UK.
M K
MK
Maconochie
I F
IF
Connerton
L A
LA
Casselton
D000085:Acetates / Q000494:pharmacology*; D019342:Acetic Acid; D000103:Acetyl-CoA Carboxylase / Q000235:genetics*; D015152:Blotting, Northern; D003001:Cloning, Molecular; D003302:Coprinus / Q000201:enzymology* / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects*; D005809:Genes, Regulator / Q000235:genetics*; D015698:Genomic Library; D007522:Isocitrate Lyase / Q000235:genetics; D010957:Plasmids / Q000235:genetics; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
D000085:Acetates; D007522:Isocitrate Lyase; D000103:Acetyl-CoA Carboxylase; D019342:Acetic Acid