Detecting changes in neuronal activities induced by N-methyl-D-aspartate receptor blockade using non-linear dynamics techniques.
The dynamics of N-methyl-D-aspartate receptor blockade-induced transitions between two types of intracellularly recorded spontaneous membrane potential oscillation from cat thalamic neurons have been studied using non-linear dynamics techniques. We report that, as previously predicted by theoretical studies, the number of degrees of freedom of these oscillations (the minimal number of independent variables governing the activity) is small, i.e. they are low dimensional. The N-methyl-D-aspartate receptor antagonists DL-2-amino-5-phosphono-valeric acid and ketamine, which transformed one type of oscillation into another, decreased the calculated dimension. DL-2-Amino-5-phosphono-valeric acid had no effect on the dimension when Mg2+ was present in the perfusion medium. The decrease in dimension was gradual and its time-course had a sigmoidal shape. It is suggested that the application of the machinery of dynamical systems theory might help to detect and monitor drug-induced membrane potential state transitions and to identify the factors underlying membrane potential oscillations.
Department of Pharmacology, University of Oxford, U.K.
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Aszodi
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Crunelli
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Soltesz
D015763:2-Amino-5-phosphonovalerate / Q000494:pharmacology*; D000818:Animals; D002415:Cats; D005071:Evoked Potentials / Q000187:drug effects; D005829:Geniculate Bodies / Q000502:physiology*; D066298:In Vitro Techniques; D007649:Ketamine / Q000494:pharmacology*; D007700:Kinetics; D008274:Magnesium / Q000494:pharmacology; D008564:Membrane Potentials / Q000187:drug effects; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D010477:Perfusion; D016194:Receptors, N-Methyl-D-Aspartate / Q000037:antagonists & inhibitors* / Q000502:physiology
D016194:Receptors, N-Methyl-D-Aspartate; D007649:Ketamine; D015763:2-Amino-5-phosphonovalerate; D008274:Magnesium