Characterization of glutamine metabolism in two related murine hybridomas.
Aspects of glutamine metabolism were examined in two related hybridomas, a high-producing line (PQX B1/2) and a low-producing line (PQX B2/2). The growth and metabolic characteristics of both cell lines were identical or very similar. During batch growth glutamine was completely exhausted from the medium and an examination of the fate of [14C]glutamine highlighted the importance of this amino acid as an energy source. The relative enzyme activities and the amount of ammonia produced during growth indicated that glutamine is oxidized preferentially via the transamination pathway. The overall rate of glutamine utilization from the growth medium was similar to the rate of [14C]glutamine uptake which suggests that transport may regulate glutamine metabolism.
Department of Biological Sciences, Manchester Polytechnic, U.K.
H A
HA
Jenkins
M
M
Butler
A J
AJ
Dickson
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D001692:Biological Transport; D002245:Carbon Dioxide / Q000378:metabolism; D002455:Cell Division; D002460:Cell Line; D005973:Glutamine / Q000378:metabolism*; D006825:Hybridomas / Q000166:cytology / Q000201:enzymology / Q000378:metabolism*; D007700:Kinetics; D051379:Mice; D010084:Oxidation-Reduction
D000911:Antibodies, Monoclonal; D005973:Glutamine; D002245:Carbon Dioxide