A new method for estimating population doubling time of vertebrate cells.
Based upon the fact that the amount of DNA doubles after each cycle, a technique was developed in which the population doubling time for cell cultures was determined from the ratio of its DNA content to [3H]thymidine incorporation under conditions in which cell numbers cannot be readily determined. This technique may be useful in determining what proportion the sample is of the total cell population.
Center for Environmental Health Sciences, Massachusetts Institute of Technology, Cambridge.
S C
SC
Tripathi
C E
CE
Donaghey
D000818:Animals; D002453:Cell Cycle; D002478:Cells, Cultured; D002645:Chickens; D004247:DNA / Q000378:metabolism*; D005347:Fibroblasts / Q000378:metabolism; D009206:Myocardium / Q000166:cytology; D013936:Thymidine / Q000378:metabolism
D004247:DNA; D013936:Thymidine