Structure and expression of the gene for Pv200, a major blood-stage surface antigen of Plasmodium vivax.
Molecular cloning and structure analysis of the gene encoding the Pv200 protein of the Sal-1 strain of Plasmodium vivax revealed an overall identity of 34-37% when the deduced amino acid sequence was compared with the sequences of various major merozoite surface antigens of Plasmodium falciparum, Plasmodium yoelii and Plasmodium chabaudi. When the Sal-1 Pv200 sequence was compared with the corresponding sequence from the Belèm strain of P. vivax, it was found that the two merozoite surface antigens were relatively well conserved with an overall amino acid sequence identity of 81%. A region of 23 repeated glutamine residues, found in the sequence of the Belèm isolate was not found, however, in the Sal-1 sequence. Amino- and carboxy-terminal domains of the Pv200 protein were expressed in the yeast Saccharomyces cerevisiae. Each recombinant protein was shown to react with antibodies in sera from splenectomized Bolivian Saimiri monkeys that had been infected previously with P. vivax, and in human sera from individuals with a history of exposure to vivax malaria. The availability of recombinant DNA-derived Pv200 proteins will now allow a full assessment of their utility in the diagnosis and immunoprophylaxis of the benign tertian malaria associated with P. vivax infection.
Chiron Corporation, Emeryville, CA 94608.
H L
HL
Gibson
J E
JE
Tucker
D C
DC
Kaslow
A U
AU
Krettli
W E
WE
Collins
M C
MC
Kiefer
I C
IC
Bathurst
P J
PJ
Barr
D000595:Amino Acid Sequence; D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood / Q000276:immunology; D000953:Antigens, Protozoan / Q000737:chemistry / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D000954:Antigens, Surface / Q000737:chemistry / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D001029:Aotus trivirgatus; D001483:Base Sequence; D003001:Cloning, Molecular; D016054:DNA, Protozoan / Q000235:genetics; D000939:Epitopes; D015870:Gene Expression; D015698:Genomic Library; D006801:Humans; D016778:Malaria, Falciparum / Q000276:immunology; D008969:Molecular Sequence Data; D010966:Plasmodium vivax / Q000235:genetics* / Q000254:growth & development; D016133:Polymerase Chain Reaction; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid
D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D000954:Antigens, Surface; D016054:DNA, Protozoan; D000939:Epitopes; C073018:Pv200 antigen, P vivax