Recovery of Clostridium thermosulfurogenes produced beta-amylase by (hydroxypropyl)methylcellulose partition.
A procedure for recovering Clostridium thermosulfurogenes produced beta-amylase from fermentation broth by partition was developed. The partition was achieved by addition of ammonium sulfate to an aqueous solution of the enzyme with (hydroxypropyl)methylcellulose. The beta-amylase-containing pellet formed upon centrifugation could be redissolved and the polymer recovered by a second salt addition. The process was not dependent on polymer/enzyme solution pH, but it was affected by temperature, polymer nominal molecular weight and loading, and fermentation carbon source. Unlike more traditional aqueous-phase partitions, such as poly(ethylene glycol)/dextran, the current approach appeared to be biospecific.
Miranda
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Department of Agricultural, Michigan State University, East Lansing 48824.
Berglund
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D002482:Cellulose; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D003013:Clostridium / Q000201:enzymology*; D004987:Ethers; D005285:Fermentation; D005782:Gels; D065347:Hypromellose Derivatives; D008747:Methylcellulose / Q000031:analogs & derivatives; D013696:Temperature; D001614:beta-Amylase / Q000302:isolation & purification*
D004987:Ethers; D005782:Gels; D065347:Hypromellose Derivatives; D002482:Cellulose; D008747:Methylcellulose; D001614:beta-Amylase