Production of Neisseria gonorrhoeae pili (fimbriae) in Pseudomonas aeruginosa.
Pseudomonas aeruginosa K/2PfS, when transformed with an expression plasmid harboring the pilin gene (pilE1) of Neisseria gonorrhoeae MS11, was able to express and assemble gonococcal pilin monomers into surface-associated pili, as judged by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, immunoblotting, and immunoelectron microscopy. Concomitant with the expression of gonococcal pili in P. aeruginosa was the virtual loss of production of P. aeruginosa K/2PfS pili normally associated with the host cell.
Division of Biomolecular Engineering, Commonwealth Scientific and Industrial Research Organisation, Parkville, Victoria, Australia.
P A
PA
Hoyne
R
R
Haas
T F
TF
Meyer
J K
JK
Davies
T C
TC
Elleman
D000595:Amino Acid Sequence; D001425:Bacterial Outer Membrane Proteins / Q000032:analysis / Q000235:genetics*; D002458:Cell Fractionation; D004591:Electrophoresis, Polyacrylamide Gel; D036781:Fimbriae Proteins; D010861:Fimbriae, Bacterial / Q000648:ultrastructure*; D005798:Genes, Bacterial; D015151:Immunoblotting; D016253:Microscopy, Immunoelectron; D008969:Molecular Sequence Data; D009344:Neisseria gonorrhoeae / Q000235:genetics* / Q000648:ultrastructure; D010957:Plasmids; D011550:Pseudomonas aeruginosa / Q000235:genetics* / Q000648:ultrastructure; D014169:Transformation, Bacterial
D001425:Bacterial Outer Membrane Proteins; D036781:Fimbriae Proteins