Physical separation of ICAM-1 binding cells.
Studies of interactions between specific molecules involved in adhesion between cells are often complicated since cells possess many different types of surface molecules influencing adhesion. To be able to further study interactions between the adhesive cell surface molecule LFA-1 and its ligand ICAM-1, we have produced an ICAM-1 fusion protein in which the extracellular part of ICAM-1 has been fused to a part of an IgG heavy chain. The fusion protein was produced by CHO cells and was easily purified in large quantities from the cell culture supernatant. The protein was coupled to magnetic beads and was shown to have binding characteristics similar to native ICAM-1. The coupled beads were used for magnetic isolation of ICAM-1 binding cells thereby providing a method for detecting changes in avidity for ICAM-1 and also for the analysis of ICAM-1 binding cells.
Hedman
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Institute of Cell and Molecular Biology, University of Umeå, Sweden.
Brändén
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Lundgren
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D000595:Amino Acid Sequence; D015703:Antigens, CD / Q000378:metabolism; D018951:Antigens, CD20; D000944:Antigens, Differentiation, B-Lymphocyte / Q000378:metabolism; D000945:Antigens, Differentiation, T-Lymphocyte / Q000378:metabolism; D001483:Base Sequence; D018801:CD2 Antigens; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D002469:Cell Separation / Q000379:methods*; D006801:Humans; D018799:Intercellular Adhesion Molecule-1; D016169:Lymphocyte Function-Associated Antigen-1 / Q000378:metabolism; D008214:Lymphocytes / Q000166:cytology* / Q000276:immunology; D008280:Magnetics; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D011971:Receptors, Immunologic / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000378:metabolism
D015703:Antigens, CD; D018951:Antigens, CD20; D000944:Antigens, Differentiation, B-Lymphocyte; D000945:Antigens, Differentiation, T-Lymphocyte; D018801:CD2 Antigens; D015815:Cell Adhesion Molecules; D016169:Lymphocyte Function-Associated Antigen-1; D009838:Oligodeoxyribonucleotides; D011971:Receptors, Immunologic; D011993:Recombinant Fusion Proteins; D018799:Intercellular Adhesion Molecule-1