Construction of a umuDC operon substitution mutation in Escherichia coli.
Using a specialized transducing lambda phage, the umuDC operon of Escherichia coli was deleted and replaced with the chloramphenicol acetyltransferase gene. The delta (umuDC)595::cat mutation was subsequently transferred by generalized P1 transduction into a variety of genetic backgrounds. It is concluded that the UmuDC proteins, which are normally required for inducible mutagenesis, are not essential for cell survival.
Section on Viruses and Cellular Biology, National Institute of Child Health and Human Development, Bethesda, MD 20892.
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D010582:Bacteriophage lambda / Q000235:genetics; D015139:Blotting, Southern; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics*; D002872:Chromosome Deletion; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D008957:Models, Genetic; D009876:Operon; D015183:Restriction Mapping; D014161:Transduction, Genetic
D004269:DNA, Bacterial; D015500:Chloramphenicol O-Acetyltransferase