Developmental switch in the pharmacology of Ca2+ channels coupled to acetylcholine release.
The pharmacological specificity of Ca2+ channel-secretion coupling in acetylcholine (ACh) and somatostatin (SOM) release was studied in the chick eye choroid neuromuscular junctions and in dissociated ciliary ganglion (CG) neurons. ACh secretion changes in development from stage (St) 40, when release is dihydropyridine (DHP) and partially omega-conotoxin (omega-CgTX) sensitive, to posthatch, when release is insensitive to DHPs but sensitive to omega-CgTX. St 40 CG neurons cultured with striated muscle have release properties similar to those of St 40 iris and choroid but different from those of St 34 neurons, which are neither DHP nor omega-CgTX sensitive. SOM (also coreleased from posthatch choroid terminals) can inhibit ACh release in both posthatch and St 40 choroids, suggesting that the SOM receptor interacts with both DHP-sensitive and -insensitive channels.
Department of Physiology and Neurobiology, University of Connecticut, Storrs 06269.
D B
DB
Gray
J L
JL
Brusés
G R
GR
Pilar
D000109:Acetylcholine / Q000378:metabolism*; D000367:Age Factors; D000818:Animals; D015220:Calcium Channels / Q000378:metabolism / Q000502:physiology*; D002478:Cells, Cultured; D002642:Chick Embryo; D002645:Chickens; D002829:Choroid / Q000502:physiology; D004558:Electric Stimulation; D066298:In Vitro Techniques; D015640:Ion Channel Gating / Q000187:drug effects; D007498:Iris / Q000502:physiology; D009469:Neuromuscular Junction / Q000502:physiology; D009543:Nifedipine / Q000494:pharmacology; D011188:Potassium / Q000494:pharmacology; D017981:Receptors, Neurotransmitter / Q000502:physiology; D017481:Receptors, Somatostatin; D013004:Somatostatin / Q000378:metabolism; D014700:Verapamil / Q000494:pharmacology
D015220:Calcium Channels; D017981:Receptors, Neurotransmitter; D017481:Receptors, Somatostatin; D013004:Somatostatin; D014700:Verapamil; D009543:Nifedipine; D000109:Acetylcholine; D011188:Potassium