Internal translation initiation in the design of improved expression vectors.
The discovery of a novel, cap-independent mechanism of translation used by picornavirus mRNAs has led to new advances in the engineering of mammalian expression vectors. It is now possible to express several proteins in a coordinate fashion from a single mRNA. Improved expression vectors suitable for virus-mediated transfer and direct DNA transfer are described.
Davies
M V
MV
Genetics Institute, Cambridge, Massachusetts.
Kaufman
R J
RJ
D000818:Animals; D001483:Base Sequence; D001709:Biotechnology; D004274:DNA, Recombinant / Q000235:genetics; D015870:Gene Expression; D005818:Genetic Engineering; D005822:Genetic Vectors; D006801:Humans; D008969:Molecular Sequence Data; D014176:Protein Biosynthesis; D012367:RNA, Viral / Q000235:genetics; D014162:Transfection
D004274:DNA, Recombinant; D012367:RNA, Viral