Correlation between ELISA and recombinant immunoblot assay in serum samples positive for anti-HCV.
To determine the false-positivity of antibody to hepatitis C virus (HCV) (anti-C100-3), the correlation between ELISA optical density (OD), anti-HCV titer and recombinant immunoblot assay (RIBA) in 87 anti-HCV-positive sera was analyzed. The results showed that > 90% of the serum samples with an anti-HCV OD > 2.0 or with titers > 10 x were RIBA reactive. These findings are applicable in daily clinical practice.
Department of Obstetrics and Gynecology, National Taiwan University, Taipei, R.O.C.
H H
HH
Lin
H Y
HY
Hsu
M H
MH
Chang
J T
JT
Wang
P J
PJ
Chen
D S
DS
Chen
D000328:Adult; D001782:Blood Donors; D002648:Child; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D016174:Hepacivirus / Q000276:immunology*; D006508:Hepatitis Antibodies / Q000032:analysis*; D006526:Hepatitis C / Q000175:diagnosis* / Q000276:immunology; D018937:Hepatitis C Antibodies; D006521:Hepatitis, Chronic / Q000175:diagnosis / Q000276:immunology; D006801:Humans; D015151:Immunoblotting; D011247:Pregnancy; D012016:Reference Values
D006508:Hepatitis Antibodies; D018937:Hepatitis C Antibodies; C078224:anti-C100-3