HLA-DP polymorphism in northern Italian celiac patients.
The contribution of HLA-DP genes to celiac disease susceptibility has been investigated in 95 Italian patients, 41 with childhood and 54 with adult disease onset. Polymerase chain reaction amplification, sequence-specific oligonucleotide probe hybridization and restriction fragment length polymorphism analyses have been carried out. All celiac patients and 56 out of 128 random healthy controls were DQw2-positive. The frequency of the DPB1*0101 allele was significantly increased (pc = 0.002, relative risk 5.21) in patients with celiac disease (23.2%) compared to the whole panel of controls (5.5%), but not to the 56 controls bearing DQw2 (10.7%). No significant difference in the frequency of DPB1*0101 was found between celiac patients with pediatric (24.4%) or adult (22.2%) onset. The DPB1*0101 allele was associated with both the DR3-DQw2 and DR7-DQw2 haplotypes. Moreover, our study has not confirmed the association with DPB1*0402 and DPB1*0301 previously reported in celiac children from southern Italy. The linkage of the DPB1*0101 allele with the DQ locus and the observation that the DP but not the DQ association appears to be ethnically dependent strongly support a secondary role of DP molecules in celiac disease susceptibility.
Mantovani
V
V
Tissue Typing Laboratory, Malpighi Hospital, Bologna, Italy.
Corazza
G R
GR
Frisoni
M
M
Zaniboni
M G
MG
Bragliani
M
M
Valentini
R A
RA
Barboni
P
P
Lambertini
A
A
Gasbarrini
G
G
D000328:Adult; D000483:Alleles; D001483:Base Sequence; D002446:Celiac Disease / Q000208:ethnology / Q000235:genetics*; D002648:Child; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006682:HLA-DP Antigens / Q000235:genetics*; D006801:Humans; D007558:Italy; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length
D006682:HLA-DP Antigens