The filter does not act as a DNA size discriminator in the neutral filter elution technique.
Various types of filters were used with the neutral filter elution technique. No significant differences in elution rate or curve shapes were found. Therefore we assume that the release of DNA fragments from the DNA gel formed by lysis of cells is the rate-determining event in elution and not the rate of migration through the filter. We suggest that the filter only serves as a support for the DNA gel.
Stockholm University, Arrhenius Laboratories for Natural Sciences, Department of Radiobiology, Sweden.
J
J
Nygren
G
G
Ahnström
D002478:Cells, Cultured; D004247:DNA / Q000737:chemistry* / Q000528:radiation effects*; D004249:DNA Damage; D004307:Dose-Response Relationship, Radiation; D005374:Filtration / Q000295:instrumentation*; D006801:Humans; D009690:Nucleic Acid Conformation
D004247:DNA