Occurrence of Listeria species in raw milk and dairy products produced in Northern Ireland.
The overall incidence of Listeria spp. in raw milk samples surveyed was found to be 25.0% (Listeria monocytogenes 15.3%), with the incidence in samples from processing centres 54.0% (L. monocytogenes 33.3%); this was higher than that in samples from dairy farms (Listeria spp. 8.8%; L. monocytogenes 5.3%). The FDA enrichment procedure was much more productive than cold enrichment and Oxford agar was superior to modified McBride agar for isolation of Listeria. Listeria monocytogenes was never isolated by direct plating of raw milk samples on Oxford agar at a detection level of 1.0 cfu/ml. Listeria spp. were isolated from 1 of 95 pasteurized milk samples (L. monocytogenes) and 1 of 33 soft cheese samples (L. seeligeri). Restriction fragment length polymorphism was more useful than sero- or phage-typing for typing of L. monocytogenes strains, and results suggest that specific L. monocytogenes strains may persist in both farm and processing environments.
Department of Agriculture for Northern Ireland, Belfast.
J
J
Harvey
A
A
Gilmour
D000818:Animals; D001434:Bacteriophage Typing; D003470:Culture Media; D003611:Dairy Products / Q000382:microbiology*; D005516:Food Microbiology; D008089:Listeria monocytogenes / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D008892:Milk / Q000382:microbiology*; D009660:Northern Ireland; D012150:Polymorphism, Restriction Fragment Length; D012703:Serotyping
D003470:Culture Media