Sulfoglycolipids bind to adhesive protein amphoterin (P30) in the nervous system.
HNK-1 antibody reactive carbohydrate epitope carried by glycolipids and glycoproteins has been shown to be involved in cell to cell interactions. It has been proposed that the HNK-1 reactive 3-sulfoglucuronyl carbohydrate epitope in glycolipids may interact with a cell surface receptor to promote the biological response in the developing nervous system. The possible occurrence of such a receptor was examined in rat nervous system. A specific binding of sulfoglycolipids to a 30 kD protein from adult rat cerebellum is described. Little binding was found with neutral glycolipids and gangliosides. The 30 kD protein from cerebellum was partially purified on a sulfatide-octyl-Sepharose affinity column. Binding of sulfoglucuronyl glycolipids to a similar 30 kD protein from forebrain previously identified as amphoterin is also shown. Amphoterin is developmentally regulated and is involved in neural cell adhesion and neurite extension.
Mohan
P S
PS
Department of Biochemistry, Eunice Kennedy Shriver Center, Waltham, MA 02254.
Laitinen
J
J
Merenmies
J
J
Rauvala
H
H
Jungalwala
F B
FB
D000818:Animals; D000943:Antigens, Differentiation / Q000276:immunology; D018959:CD57 Antigens; D002352:Carrier Proteins / Q000302:isolation & purification / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D002531:Cerebellum / Q000378:metabolism*; D002846:Chromatography, Affinity; D003600:Cytosol / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000032:analysis; D005260:Female; D006017:Glycolipids / Q000378:metabolism*; D024243:HMGB1 Protein; D006609:High Mobility Group Proteins / Q000302:isolation & purification / Q000378:metabolism*; D008715:Methionine / Q000378:metabolism; D008970:Molecular Weight; D009419:Nerve Tissue Proteins / Q000378:metabolism; D016548:Prosencephalon / Q000378:metabolism; D011485:Protein Binding; D051381:Rats; D011919:Rats, Inbred Strains; D014316:Tritium
D000943:Antigens, Differentiation; D018959:CD57 Antigens; D002352:Carrier Proteins; D000939:Epitopes; D006017:Glycolipids; D024243:HMGB1 Protein; D006609:High Mobility Group Proteins; D009419:Nerve Tissue Proteins; C011117:sulfoglycolipids; D014316:Tritium; D008715:Methionine