Isolation of restriction-reduced mutants from Streptomyces.
Restriction-reduced mutants were isolated from Streptomyces rosa subsp. notoensis KA301 and S. tanashiensis strain Kala which produce the benzoisochromanequinone antibiotics nanaomycin and kalafungin, respectively. The mutants of S. rosa, which can be transformed with a multi-copy plasmid and in which the actinophage Pa16 can propagate, were selected. They were transformed with a single-copy plasmid propagated in S. lividans TK24, and with its modified plasmid propagated in the mutant at higher efficiency. The mutants of S. tanashiensis were selected by their capability to be transformed with a multi-copy plasmid. The efficiency of transformation with a single-copy plasmid propagated in S. lividans TK24 was low, but was much increased by heating the protoplasts at 42 degrees C for 15 min prior to the transformation. These mutants derived from both strains probably lack at least one of their restriction systems.
School of Pharmaceutical Sciences, Kitasato University, Tokyo, Japan.
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Kakinuma
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Ikeda
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Omura
D004926:Escherichia coli / Q000235:genetics; D006358:Hot Temperature; D008769:Methylnitronitrosoguanidine / Q000494:pharmacology; D016296:Mutagenesis; D010957:Plasmids; D011523:Protoplasts; D013302:Streptomyces / Q000235:genetics / Q000302:isolation & purification*; D014169:Transformation, Bacterial
D008769:Methylnitronitrosoguanidine