Neuronal changes in the nigrostriatal pathway of 1-methyl-4-phenylpyridine-treated mice.
Thirty young-adult mice were treated with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) 30 mg/kg/day for 2 days and sacrificed 24 hours later in order to determine striatal catecholamines and to study morphological changes in the nigrostriatal pathway. Immunohistological techniques were also used with polyclonal antibodies for glial fibrillary acidic protein (GFAP) and tyrosine hydroxylase (TH), and monoclonal antibodies for two subunits of neurofilaments. Silver impregnation demonstrated conspicuous neuronal changes affecting cellular processes from substantia nigra in all treated mice. Terminal and axonal degeneration were also observed in striata. These changes were associated with a moderate to marked gliosis. The TH immunoreactivity was normal in cell bodies of substantia nigra but was decreased in striata from MPTP-treated mice. These data indicate that in mice the deterioration of dendritic and axonal neuropil may constitute a significant causal factor of MPTP neurotoxicity.
Neurological Tissue Bank, University of Barcelona, Spain.
M L
ML
Boatell
N
N
Mahy
A
A
Cardozo
S
S
Ambrosio
E
E
Tolosa
F F
FF
Cruz-Sánchez
D015632:1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine / Q000494:pharmacology*; D000818:Animals; D001369:Axons / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D002395:Catecholamines / Q000378:metabolism; D003342:Corpus Striatum / Q000166:cytology* / Q000187:drug effects; D003712:Dendrites / Q000276:immunology / Q000378:metabolism; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009434:Neural Pathways / Q000166:cytology / Q000187:drug effects; D009474:Neurons / Q000187:drug effects*; D013378:Substantia Nigra / Q000166:cytology* / Q000187:drug effects; D014446:Tyrosine 3-Monooxygenase / Q000276:immunology / Q000378:metabolism
D002395:Catecholamines; D005904:Glial Fibrillary Acidic Protein; D015632:1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine; D014446:Tyrosine 3-Monooxygenase