[Diagnosis of ocular chlamydiosis by gene amplification (polymerase chain reaction or P.C.R.)].
Identification of conjunctival chlamydioses could be very difficult since this disease is extremely latent. Moreover previous instillation of topical antibiotic provokes a negativation of D.I.F. and of cultures. P.C.R. is very useful, it was demonstrative in 41 cases out of 47 cases; D.I.F. was positive only in 18 cases. P.C.R. is a very promising method to recognize chlamydiae; identification of different serovars is processing.
C.H.R., Hôpital des Enfants, Service d'Ophtalmologie, Bordeaux.
P
P
Vérin
P
P
Gendre
P
P
Goldschmidt
B
B
Mortemousque
P
P
Coulon
W
W
Williamson
P A
PA
Ndiaye
D002690:Chlamydia Infections / Q000175:diagnosis* / Q000382:microbiology; D002692:Chlamydia trachomatis / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000032:analysis; D015818:Eye Infections, Bacterial / Q000175:diagnosis* / Q000382:microbiology; D006801:Humans; D016133:Polymerase Chain Reaction
D004269:DNA, Bacterial
Diagnostic des chlamydioses oculaires par amplification génique (réaction en chaîne à la polymérase ou P.C.R.).