Localization of alpha-2 adrenergic receptors in the human eye.
To elucidate sites of the action of alpha-2 adrenergic drugs, we localized binding sites for a potent alpha-2 adrenergic agonist, UK-14,304, in sections of the human cadaveric eye by an in vitro ligand-binding technique and autoradiography. Its specific binding sites were found in the iris epithelium and ciliary epithelium at a high level, and also in the ciliary muscle, retina, retinal pigment epithelium (and/or choroid). Binding of UK-14,304 to the ocular pigments was prevented by preincubating the eye sections with chloroquine, which reduced the non-specific binding to a low level.
Matsuo
T
T
Department of Ophthalmology, University of British Columbia, Vancouver, Canada.
Cynader
M S
MS
D000316:Adrenergic alpha-Agonists / Q000378:metabolism; D000368:Aged; D001345:Autoradiography; D000068438:Brimonidine Tartrate; D005123:Eye / Q000187:drug effects / Q000378:metabolism*; D005260:Female; D006801:Humans; D008024:Ligands; D008297:Male; D008875:Middle Aged; D011810:Quinoxalines / Q000378:metabolism; D011942:Receptors, Adrenergic, alpha / Q000378:metabolism*
D000316:Adrenergic alpha-Agonists; D008024:Ligands; D011810:Quinoxalines; D011942:Receptors, Adrenergic, alpha; D000068438:Brimonidine Tartrate