Production of desferrioxamine E and new analogues by directed fermentation and feeding fermentation.
Streptomyces olivaceus TU 2718 produces the siderophore desferrioxamine E. Production depends on L-lysine and iron concentrations in the medium. With optimized conditions the yield of desferrioxamine E could be increased to 12 g/l in feeding fermentations. Supplementation of the basic production medium with natural and synthetic precursors of desferrioxamine E led to the production of twelve new analogues of desferrioxamine E.
Lehrbereich Mikrobiologie/Antibiotika, Universität Tübingen, Federal Republic of Germany.
J
J
Meiwes
H P
HP
Fiedler
H
H
Zähner
S
S
Konetschny-Rapp
G
G
Jung
D001431:Bacteriological Techniques; D002851:Chromatography, High Pressure Liquid; D003470:Culture Media; D003676:Deferoxamine / Q000302:isolation & purification / Q000378:metabolism*; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D007501:Iron / Q000378:metabolism; D008239:Lysine / Q000378:metabolism; D013302:Streptomyces / Q000254:growth & development / Q000378:metabolism*; D013395:Sucrose / Q000378:metabolism
D003470:Culture Media; D013395:Sucrose; D007501:Iron; D003676:Deferoxamine; D008239:Lysine