[Ultrastructural localization of myelin bodies and acid phosphatase activities in the liver and kidney induced by quinacrine in rats].
Electron microscopic studies were conducted to reveal the ultrastructural aspects of the myelin body and acid phosphatase activity in rats induced by quinacrine, an antimalarial drug. Each of 22 rats in three groups were examined. The first group of control rats was initially given a single i.p. dose (200 mg/kg) of diethylnitrosamine (DEN) only and then fed a CRF-1 basal diet for 8 weeks. The second and third group were treated with DEN or saline, and starting 2 weeks later, were fed a CRF-1 basal diet supplemented with 500 ppm quinacrine for 6 weeks. All animals were subjected to a partial hepatectomy at week 3, and then sacrificed at week 8. Liver and kidney tissues specimens from 2 rats per group were collected for routine electron microscopic study. Furthermore, the activity of acid phosphatase, a key enzyme for lysosomal activity, was also investigated. In this study, tissues were fixed with a solution of 2.5% glutaraldehyde in 0.1 M sodium cacodylate buffer. After fixation, tissues were frozen and their 8 microns sections were treated with Gomori's lead nitrate buffer solution, and post-fixed with a 1% osmium solution. After being embedded in Epon 812, ultrathin sections were made. Intracellular myelin bodies were observed in the hepatocytes, interlobular bile duct cells, renal glomerular podocytes and renal tubular cells in the quinacrine treated groups, but not were observed in rats treated with DEN alone.(ABSTRACT TRUNCATED AT 250 WORDS)
Imazawa
T
T
Furukawa
F
F
Yoshida
J
J
Shinoda
K
K
Imaida
K
K
Takahashi
M
M
Hayashi
Y
Y
D000135:Acid Phosphatase / Q000378:metabolism*; D000818:Animals; D006651:Histocytochemistry; D002479:Inclusion Bodies / Q000737:chemistry*; D007668:Kidney / Q000201:enzymology / Q000648:ultrastructure*; D008099:Liver / Q000201:enzymology / Q000648:ultrastructure*; D008297:Male; D008854:Microscopy, Electron; D009185:Myelin Proteins / Q000032:analysis*; D011796:Quinacrine / Q000494:pharmacology*; D051381:Rats; D011916:Rats, Inbred F344
D009185:Myelin Proteins; D000135:Acid Phosphatase; D011796:Quinacrine