Establishment and characterization of a cell line of congenital primitive neuroectodermal tumor of soft tissue.
A new human cell line, termed Muraoka, has been established from the recurrent tumor of a case of congenital primitive neuroectodermal tumor (PNET) arising at the temporofacial region of a male infant. The microscopic findings of this cell line were epithelioid, and the xenografted tumor in a nude mouse consisted of the malignant epithelioid cells. Immunohistochemically, the cells were positive for neuron-specific enolase, S-100 protein, carcinoembryonic antigen, cytokeratin, epithelial membrane antigen, and glial fibrillary acidic protein. These findings were quite similar to those of the epithelioid cells in the original tumor and of the xenografted tumor cells. Neither chromosomal abnormalities nor N-myc amplification were observed. Morphological differentiation after treatment with N6-2'-O-dibutyryladenosine 3':5'-cyclic monophosphate (Bt2-cAMP), all-trans-retinoic acid (RA), prostaglandin E1 (PGE1), and 5-bromo-2'-deoxyuridine (BrdU) showed two different results. Bt2-cAMP and PGE1 induced neuronal differentiation with the extension of neurites, whereas RA and BrdU predominantly induced Schwannian differentiation (flat cells). In these respects, the cell line Muraoka seems to be useful for studying characteristics of PNET as well as for developing the new treatments against such tumors.
Department of Pediatric Surgery, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
T
T
Fukushige
M
M
Nagoshi
Y
Y
Hachitanda
T
T
Ueno
Y
Y
Zaizen
S
S
Suita
M
M
Tsuneyoshi
Y
Y
Kaneko
A
A
Nakagawara
D000818:Animals; D001973:Bromodeoxyuridine / Q000494:pharmacology; D003994:Bucladesine / Q000494:pharmacology; D002272:Carcinoembryonic Antigen / Q000032:analysis; D002460:Cell Line / Q000187:drug effects; D005153:Facial Neoplasms / Q000151:congenital* / Q000378:metabolism / Q000473:pathology; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D006801:Humans; D007231:Infant, Newborn; D007621:Karyotyping; D008297:Male; D051379:Mice; D008819:Mice, Nude; D016501:Neurites / Q000187:drug effects; D009447:Neuroblastoma / Q000151:congenital* / Q000378:metabolism / Q000473:pathology; D018241:Neuroectodermal Tumors, Primitive, Peripheral / Q000151:congenital* / Q000378:metabolism / Q000473:pathology; D010751:Phosphopyruvate Hydratase / Q000032:analysis; D009418:S100 Proteins / Q000032:analysis; D014212:Tretinoin / Q000494:pharmacology
D002272:Carcinoembryonic Antigen; D005904:Glial Fibrillary Acidic Protein; D009418:S100 Proteins; D014212:Tretinoin; D003994:Bucladesine; D010751:Phosphopyruvate Hydratase; D001973:Bromodeoxyuridine