Effect of gamma-glutamyl transpeptidase inhibitors on the transport of glutamate into neuronal and glial primary cultures.
Inhibitors of gamma-glutamyl transpeptidase (mixture of serine and borate--13 mM, kainic acid--5 mM and 6-diazo-5-oxo-L-norleucine--2 mM) significantly suppressed glutamate uptake into cultured neurones and glial cells. The simultaneous application of any of these inhibitors with ouabain resulted in a further decline in glutamate uptake. It can be speculated that gamma-glutamyl transpeptidase significantly contributes to glutamate transport into nerve cells in the early period of brain development until the Na(+)-K(+)-gradient is fully constituted.
Institute of Physiology, Czechoslovak Academy of Sciences, Prague.
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Jankásková
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Lisý
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Stastný
D000375:Aging / Q000378:metabolism; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D001881:Borates / Q000494:pharmacology*; D001921:Brain / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D002478:Cells, Cultured; D002645:Chickens; D003980:Diazooxonorleucine / Q000494:pharmacology*; D005971:Glutamates / Q000378:metabolism*; D018698:Glutamic Acid; D007608:Kainic Acid / Q000494:pharmacology*; D009457:Neuroglia / Q000187:drug effects / Q000378:metabolism*; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D012694:Serine / Q000494:pharmacology*; D005723:gamma-Glutamyltransferase / Q000037:antagonists & inhibitors*
D001881:Borates; D005971:Glutamates; C040900:serine-borate complex; D003980:Diazooxonorleucine; D018698:Glutamic Acid; D012694:Serine; D005723:gamma-Glutamyltransferase; D007608:Kainic Acid