Protein engineering for thermostability.
Studies with small, monomeric proteins indicate that, to some extent, the effects of amino acid substitutions can be predicted. However, conformational and other changes may complicate the prediction. Site-directed mutagenesis is leading both to a better understanding of protein stability and to the production of more stable proteins.
Nosoh
Y
Y
Department of Fundamental Science, College of Science and Engineering, Iwaki Meisei University, Fukushima, Japan.
Sekiguchi
T
T
D000595:Amino Acid Sequence; D004355:Drug Stability; D005818:Genetic Engineering; D006358:Hot Temperature; D008969:Molecular Sequence Data; D009154:Mutation; D011487:Protein Conformation; D011506:Proteins / Q000235:genetics* / Q000378:metabolism
D011506:Proteins