Halide permeation through 10 pS and 20 pS anion channels in human airway epithelial cells.
Halide permeability sequences were obtained from reversal potential measurements of single-channel currents through 10 pS and 20 pS anion channels in human airway epithelial cells. The sequences obtained were Cl- greater than I- greater than Br- greater than or equal to F- for the 10 pS channel and Cl- greater than I- greater than or equal to Br- greater than or equal to F- for the 20 pS channel. However, the permeability differences were not large, the greatest being 0.66 for the ratio of fluoride to chloride permeability in the 20 pS channel. Single-channel currents were also measured with solutions of constant halide concentration but varying ratios of chloride to fluoride ions. An anomalous mole fraction effect was observed for the 20 pS channel but not for the 10 pS channel, suggesting that the former is a multi-ion channel. Comparison of the halide permeability sequences of these two channels with those of whole-cell currents in other epithelial cells does not support their involvement in any of the known whole-cell epithelial currents.
Wilk-Blaszczak
M A
MA
Department of Physiology, University of Alberta, Edmonton, Canada.
French
A S
AS
Man
S F
SF
D001965:Bromides / Q000378:metabolism; D002463:Cell Membrane Permeability; D002478:Cells, Cultured; D002712:Chlorides / Q000378:metabolism; D004847:Epithelial Cells; D004848:Epithelium / Q000378:metabolism / Q000502:physiology; D005459:Fluorides / Q000378:metabolism; D006801:Humans; D007454:Iodides / Q000378:metabolism; D007473:Ion Channels / Q000378:metabolism*; D008564:Membrane Potentials; D009297:Nasal Mucosa / Q000166:cytology / Q000378:metabolism* / Q000502:physiology
D001965:Bromides; D002712:Chlorides; D007454:Iodides; D007473:Ion Channels; D005459:Fluorides