Replication origins and pause sites in sea urchin mitochondrial DNA.
We have used a combination of one- and two-dimensional agarose gel electrophoresis, and solution hybridization to strand-specific probes, to map the replication origin of sea urchin mitochondrial DNA and to investigate the structure of replication intermediates. These assays are consistent with replication initiating unidirectionally from the D-loop region by D-loop expansion, as in vertebrates. A prominent site of initiation of lagging-strand synthesis lies at, or near to, the boundary between the genes for ATPase 6 and COIII, which is also close to a pause site for leading-strand synthesis. These findings suggest a role for pause sites in the regulation of mitochondrial transcription and replication, possibly involving template-binding proteins.
Department of Genetics, University of Glasgow, U.K.
A G
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Mayhook
A M
AM
Rinaldi
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HT
Jacobs
D000818:Animals; D002874:Chromosome Mapping; D004261:DNA Replication; D004272:DNA, Mitochondrial / Q000235:genetics* / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000235:genetics; D003576:Electron Transport Complex IV / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D015180:Electrophoresis, Gel, Two-Dimensional; D046911:Macromolecular Substances; D009245:NADH Dehydrogenase / Q000235:genetics; D009690:Nucleic Acid Conformation; D012335:RNA, Ribosomal / Q000235:genetics; D012343:RNA, Transfer / Q000235:genetics; D015183:Restriction Mapping; D012617:Sea Urchins / Q000235:genetics*
D004272:DNA, Mitochondrial; D004275:DNA, Ribosomal; D046911:Macromolecular Substances; D012335:RNA, Ribosomal; D012343:RNA, Transfer; D009245:NADH Dehydrogenase; D003576:Electron Transport Complex IV