The use of macroporous gelatin carriers for the cultivation of mammalian cells in fluidised bed reactors.
A fluidised bed system for the cultivation of mammalian cells on a new type of macroporous gelatin microcarrier is described. The volumetric cell densities achieved under controlled conditions for two 'standard cell lines' (VERO, CHO) were one order of magnitude higher compared to conventional techniques using spherical microcarriers. The system can be potentially used for both anchorage dependent and independent cells.
Reiter
M
M
Department of Biotechnology, University of Agriculture, Vienna, Austria.
Hohenwarter
O
O
Gaida
T
T
Zach
N
N
Schmatz
C
C
Blüml
G
G
Weigang
F
F
Nilsson
K
K
Katinger
H
H
D000818:Animals; D002448:Cell Adhesion; D002449:Cell Aggregation; D002455:Cell Division; D002478:Cells, Cultured / Q000166:cytology*; D005780:Gelatin; D008722:Methods; D008863:Microspheres; D010477:Perfusion
D005780:Gelatin