The locus Om, responsible for the DDK syndrome, maps close to Sigje on mouse chromosome 11.
The DDK inbred strain of mouse has a striking particularity: when DDK females are crossed to males of other strains they exhibit a reduced fertility, whereas the reciprocal crosses (non-DDK females x DDK males) are fertile (Wakasugi et al. 1967; Wakasugi 1973). The low fertility results from an early embryonic lethality, the F1 embryos dying near the late morula-early blastocyst stage. Genetic analyses (Wakasugi 1974) and nuclear and cytoplasmic transfers (Renard and Babinet 1986; Babinet et al. 1990; Mann 1986), have shown that the failure of the embroys to develop is due to an incompatibility between a DDK maternally encoded cytoplasmic product and the non-DDK paternal genome. In order to elucidate the genetic determinism of this embryonic lethality, we have analyzed the fertility of male progeny from a backcross BALB/c females x (BALB/c x DDK)F1 males and that of males from a set of recombinant inbred (RI) strains, established from DDK and BALB/c progenitors, when mated with DDK females. Our results indicate that a single locus, Om, is responsible for the DDK syndrome and is located on Chromosome (Chr) 11, very close to the Sigje locus.
Baldacci
P A
PA
Unité de Génétique des Mammifères, Institut Pasteur, Paris, France.
Richoux
V
V
Renard
J P
JP
Guénet
J L
JL
Babinet
C
C
D000818:Animals; D015139:Blotting, Southern; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D004276:DNA, Satellite; D005260:Female; D005298:Fertility / Q000235:genetics*; D008040:Genetic Linkage; D005819:Genetic Markers; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008815:Mice, Inbred Strains; D012150:Polymorphism, Restriction Fragment Length; D013577:Syndrome
D004276:DNA, Satellite; D005819:Genetic Markers