The importance of P and type 1 fimbriae for the persistence of Escherichia coli in the human gut.
The faecal Escherichia coli flora was studied in 89 infants. Each infant was followed with a mean of 12 faecal samples (range 5-21) between 0 and 18 months of age. All isolates were assayed for P fimbriae and biochemically phenotyped and the persistence of each strain (phenotype) in the infant's gut was determined. In a subset of strains the occurrence of type 1 fimbriae and adherence to HeLa cells was studied. Thirty-one per cent of isolates belonging to strains colonizing for longer than 6 months expressed P fimbriae compared to 19% of the isolates from strains colonizing 1-6 months or transient strains colonizing less than 1 month. Type 1 fimbriae and adherence to HeLa cells occurred similarly often in all groups of strains. We conclude that P fimbriae, but not type 1 fimbriae or HeLa cell adherence seemed to contribute to the ability of the E. coli strain to colonize the human intestine.
Department of Pediatrics, Karolinska Institute, S:t Göran's Children's Hospital, Stockholm, Sweden.
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K
Tullus
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Kühn
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Orskov
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Orskov
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Möllby
D001422:Bacterial Adhesion / Q000502:physiology; D004926:Escherichia coli / Q000145:classification / Q000254:growth & development*; D005243:Feces / Q000382:microbiology; D010861:Fimbriae, Bacterial / Q000502:physiology*; D006367:HeLa Cells / Q000382:microbiology; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D007422:Intestines / Q000382:microbiology*; D010641:Phenotype; D011971:Receptors, Immunologic; D012703:Serotyping / Q000379:methods
D011971:Receptors, Immunologic; C037872:pili, bacterial receptor