Separation and purification of IgG1 and IgG2 from IgG-Cohn-II fraction of human plasma by pseudobioaffinity chromatography on histidyl-AH-sepharose.
L-histidine coupled to aminohexyl-sepharose (H-AH) has been used as an affinity sorbent to separate IgG from human plasma. Two subclasses IgG1 and IgG2 were specifically bound to histidyl-AH-sepharose at pH 7.4 and eluted using 0.2 M and 1M NaCl. The specificity of the two subclasses were determined by immunoelectrophoresis. Quantitative determination of IgG1, IgG2 was carried out using radial immunodiffusion technique.
Département de Génie Biologique, Université de Technologie de Compiègne, France.
A
A
el-Kak
M A
MA
Vijayalakshmi
D002846:Chromatography, Affinity; D004587:Electrophoresis, Agar Gel; D006639:Histidine / Q000031:analogs & derivatives*; D006801:Humans; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D007074:Immunoglobulin G / Q000097:blood / Q000145:classification / Q000302:isolation & purification*; D007526:Isoelectric Point; D012680:Sensitivity and Specificity; D012685:Sepharose / Q000031:analogs & derivatives*
D007074:Immunoglobulin G; C080392:histidyl-AH-sepharose; D006639:Histidine; D012685:Sepharose