Molecular cytogenetic analysis of a complex t(10;22;11) translocation in Ewing's sarcoma.
Fluorescence in situ hybridization (FISH) using chromosome-specific plasmid libraries and chromosome region-specific DNA markers allowed the characterization of a t(10;22;11) (p11.2;q12;q24) in a Ewing's sarcoma (ES). This study illustrates the usefulness of molecular cytogenetic analysis of ES, especially for determining the localization of the translocated 11q24-25 segment in complex or variant translocations.
Speleman
F
F
Department of Medical Genetics, University Hospital, Ghent, Belgium.
Van Roy
N
N
Wiegant
J
J
Dierick
A M
AM
Uyttendaele
D
D
Leroy
J G
JG
D000328:Adult; D001859:Bone Neoplasms / Q000235:genetics*; D002879:Chromosomes, Human, Pair 10; D002880:Chromosomes, Human, Pair 11; D002892:Chromosomes, Human, Pair 22; D002898:Chromosomes, Human, Pair 8; D005260:Female; D006801:Humans; D007621:Karyotyping; D008856:Microscopy, Fluorescence; D009693:Nucleic Acid Hybridization; D012512:Sarcoma, Ewing / Q000235:genetics*; D013977:Tibia / Q000473:pathology; D014178:Translocation, Genetic; D014407:Tumor Cells, Cultured