Upregulation of adhesion molecules induced by broncho-vaxom on phagocytic cells.
Whole blood was incubated with the bacterial extract Broncho-Vaxom (OM85) at various concentrations and for different periods of time. Expression of the beta 2-integrins (LFA-1, CD11a/CD18; MAC-1, CD11b/CD18; p150,95, CD11c/CD18) and ICAM-1 (CD54) by monocytes and granulocytes was studied using flow cytometry. OM85 enhanced the expression of MAC-1 and ICAM-1 on monocytes and granulocytes in a dose-dependent manner. Maximal expression was achieved with 1 mg/ml bacterial extract. The effect on MAC-1 expression was not due to the low concentration of endotoxin contaminating the preparation (less than 1 ng/mg) since polymyxin-B did not substantially affect the adhesion molecule upregulation induced by OM85. In addition, OM85 enhanced the expression of p150,95 on monocytes and granulocytes, and also increased expression of LFA-1 on monocytes, but not on granulocytes. While MAC-1 and p150,95 expression reached peak values between 1 and 6 h, levels of ICAM-1 rose constantly for 10 h. We suggest that the clinical interest of OM85 in the management of recurrent infections could be related to be upregulation of adhesion molecules induced by this bacterial extract.
Duchow
J
J
Department of Immunology, Hopital Erasme, Université Libre de Bruxelles, Belgium.
Marchant
A
A
Delville
J P
JP
Schandene
L
L
Goldman
M
M
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D001419:Bacteria; D015815:Cell Adhesion Molecules / Q000032:analysis*; D002457:Cell Extracts; D006098:Granulocytes / Q000737:chemistry / Q000187:drug effects; D006801:Humans; D066298:In Vitro Techniques; D016023:Integrins / Q000032:analysis*; D018799:Intercellular Adhesion Molecule-1; D016177:Macrophage-1 Antigen / Q000032:analysis; D009000:Monocytes / Q000737:chemistry / Q000187:drug effects; D010586:Phagocytes / Q000737:chemistry / Q000187:drug effects*; D015854:Up-Regulation; D000095983:Bacterial Lysates
D000276:Adjuvants, Immunologic; D015815:Cell Adhesion Molecules; D002457:Cell Extracts; D016023:Integrins; D018799:Intercellular Adhesion Molecule-1; D016177:Macrophage-1 Antigen; C030259:Broncho-Vaxom; D000095983:Bacterial Lysates