Clonal derivatives of a human B-lymphoblastoid cell line producing prolactin--a cytogenetic characterization.
The IM-9-P cell line is a variant of the human B-lymphoblastoid cell line IM-9 which ectopically secretes prolactin (hPRL). The heterogeneous line IM-9-P and three sublines of clonal origin, two of them positive and one negative for PRL gene expression, were subjected to cytogenetic analysis and compared with the reference line IM-9 which showed a normal female diploid karyotype. G-banding revealed several rearrangements in the chromosomes. Nine altered chromosomes including one stable marker chromosome were common to all analysed karyotypes of IM-9-P cells and their clones. A second marker chromosome 'mar2' occurred only in the karyotypes of the hPRL producing clones, but not in the non-producing clone. None of the visible alterations involve chromosome 6 which carries the PRL gene in humans.
Institute of Hormone and Fertility Research, Hamburg, FRG.
A
A
Bonhoff
B
B
Gellersen
K R
KR
Held
H G
HG
Bohnet
D001402:B-Lymphocytes / Q000378:metabolism* / Q000648:ultrastructure; D002460:Cell Line; D002869:Chromosome Aberrations; D002871:Chromosome Banding; D003582:Cytogenetics; D005260:Female; D015870:Gene Expression; D006801:Humans; D007231:Infant, Newborn; D007621:Karyotyping; D009101:Multiple Myeloma; D011388:Prolactin / Q000235:genetics / Q000378:metabolism*
D011388:Prolactin