Expression of human lymphotoxin in Namalwa KJM-1 cells adapted to serum-free medium.
A Namalwa cell line, KJM-1, which was adapted to serum-free medium is thought to be a good host cell line for recombinant DNA technology. We previously reported the expression of human beta-interferon (beta-IFN) in Namalwa KJM-1 (Miyaji, 1989a). The utility of Namalwa KJM-1 for expression of foreign genes was further examined. As a target gene to be expressed, human lymphotoxin (hLT) cDNA was used. It was engineered for expression in Namalwa KJM-1 using a simian virus 40 (SV40)-based expression vector pAGE107 (Miyaji, 1989a). It contains all components necessary for the expression of cDNA in mammalian cells. The expression vector was introduced into Namalwa KJM-1 by electroporation. Among the transformants, clone 7 was further examined for the expression of hLT in serum-free medium. The production level of hLT was augmented with the increase of the cell density. Thus it was further indicated that Namalwa KJM-1 is useful for production of foreign gene products.
Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., Japan.
H
H
Miyaji
N
N
Harada
T
T
Mizukami
S
S
Sato
N
N
Fujiyoshi
S
S
Itoh
D002460:Cell Line / Q000378:metabolism*; D003470:Culture Media; D004560:Electricity; D005822:Genetic Vectors; D006801:Humans; D008233:Lymphotoxin-alpha / Q000096:biosynthesis*; D011994:Recombinant Proteins / Q000096:biosynthesis; D013539:Simian virus 40; D014162:Transfection
D003470:Culture Media; D008233:Lymphotoxin-alpha; D011994:Recombinant Proteins