Conditions for assembly of tubulin-based structures in unfertilized sea urchin eggs. Spirals, monasters and cytasters.
Cytasters were induced in the unfertilized eggs of the sea urchin Lytechinus pictus by two different methods: (1) treatment with hexylene glycol or taxol, which are known to lower the tubulin critical concentration in vitro, but do not activate the nuclear cycle, and (2) by raising the cytoplasmic pH from that of unfertilized cytoplasm to that of fertilized, which activated the nuclear cycle and initiated tubulin polymerization. In the unactivated eggs, with increasing concentrations of the inducing agents, temperature and duration of treatment, microtubule structures that formed showed a progression from loose microtubule networks and spiral arrays to tightly organized cytasters. In eggs incubated in sea water containing 2.5 or 10 mM ammonium acetate titrated with HCl or NaOH in steps from pH 5.0 to pH 9.0, the nuclear cycle and tubulin polymerization were initiated at about pH 7.0. The degree of development attainable after three hours was dependent on the pH, with spirals forming at the threshold level of pH 7.0, monasters at pH 7.5, and at pH 8.5 cells formed cytasters, multipolar spindles and even completed multipolar divisions. In unactivated eggs, tubulin was made available by lowering of the tubulin critical concentration; in activated eggs it was made available from some previously unavailable store. The evidence suggests that the amount of assembly-competent tubulin available, regardless of how it is made available, determines the type of structure that is formed, with microtubule bundles and spirals at the lower concentrations and functional cytasters at the upper. We also describe some details of cytaster formation, including the role of microtubule interactions and the movement of dense granules to the aster centers in hexylene glycol-treated eggs.
Harris
P J
PJ
Department of Biology, University of Oregon, Eugene 97403.
Clason
E L
EL
D000470:Alkaloids / Q000494:pharmacology; D000818:Animals; D001704:Biopolymers; D003595:Cytoplasmic Streaming / Q000502:physiology; D006018:Glycols / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D008870:Microtubules / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure*; D009865:Oocytes / Q000187:drug effects / Q000648:ultrastructure*; D017239:Paclitaxel; D012617:Sea Urchins; D013696:Temperature; D014404:Tubulin / Q000378:metabolism*; D015053:Zygote
D000470:Alkaloids; D001704:Biopolymers; D006018:Glycols; D014404:Tubulin; C011480:hexylene glycol; D017239:Paclitaxel