Control of enzyme properties in supramolecular systems.
The study deals with stability and activity of enzymes in supramolecular systems. Acid phosphatase (EC 3.1.3.2) has been studied as model enzyme. The organic phase is rich in C2-C4 acetates. Didodecyldimethylammonium chloride (DD-DACl) has been mainly used as ionic surfactants. The rate of enzyme inactivation is smaller than in buffer and is less dependent on storage temperature. Specific activity of the enzyme is lowered because of a less affinity towards the substrate and of reduction of maximal velocity.
Alfani
F
F
Department of Chemistry, Chemical Engineering and Materials, University of L'Aquila, Italy.
Cantarella
M
M
Cutarella
N
N
Spreti
N
N
Germani
R
R
Savelli
G
G
D000135:Acid Phosphatase / Q000737:chemistry; D001709:Biotechnology; D002384:Catalysis; D004795:Enzyme Stability; D004798:Enzymes / Q000737:chemistry*; D007700:Kinetics; D008956:Models, Chemical; D012995:Solubility; D013501:Surface-Active Agents; D014867:Water
D004798:Enzymes; D013501:Surface-Active Agents; D014867:Water; D000135:Acid Phosphatase