Esterase heterogeneity in mussel Mytilus galloprovincialis: effects of organophosphate and carbamate pesticides in vitro.
1. In mussel Mytilus galloprovincialis tissue-specific electrophoretic patterns of multiple molecular forms of esterases were observed. 2. Carboxylesterases and acetylesterases were identified following the application of pertinent substrates and inhibitors. 3. Hepatopancreas was the richest source of esterase activity followed by gills, ovaria, adductor muscle and mantle. 4. In vitro studies indicated that organophosphate and carbamate insecticides have distinct effects on different esterase isozymes. Esterases classified as carboxylesterase were more sensitive to parathion and particularly to paraoxon.
Ozretić
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B
Institute Rudjer Bosković, Center for Marine Research, Rovinj, Croatia.
Krajnović-Ozretić
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M
D000115:Acetylesterase / Q000378:metabolism*; D000818:Animals; D049872:Bivalvia / Q000187:drug effects / Q000201:enzymology*; D002219:Carbamates / Q000633:toxicity*; D002265:Carboxylic Ester Hydrolases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D005260:Female; D005880:Gills / Q000187:drug effects; D066298:In Vitro Techniques; D008099:Liver / Q000187:drug effects; D009943:Organophosphorus Compounds / Q000633:toxicity*; D010053:Ovary / Q000187:drug effects; D010179:Pancreas / Q000187:drug effects; D010261:Paraoxon / Q000633:toxicity; D010278:Parathion / Q000633:toxicity
D002219:Carbamates; D009943:Organophosphorus Compounds; D010278:Parathion; D002265:Carboxylic Ester Hydrolases; D000115:Acetylesterase; D010261:Paraoxon