Close linkage of the olfactory marker protein gene to the mouse deafness mutation shaker-1.
One thousand sixty-six progeny have been generated from a backcross segregating for the mouse deafness mutation, shaker-1 (sh-1). One thousand fifty-two mice were analyzed for a protein polymorphism segregating for the distal flanking marker, beta-globin (Hbb), and 13 recombinants between Hbb and sh-1 were identified. One thousand eight mice were analyzed for a restriction fragment length polymorphism segregating for the proximal flanking marker, tyrosinase (c), and 54 recombinants between c and sh-1 were identified, completing a panel of 67 recombinant mice from the backcross in the vicinity of the sh-1 mutation. This panel allows the identification of markers closely linked to the sh-1 mutation that may act as start points for a chromosomal walk to the gene. One such marker, the olfactory marker protein gene (Omp), is recombinant with sh-1 in only one mouse from the recombinant panel. Thus, the Omp gene lies 0.1 cM from sh-1, on average, a distance of 200 kb. Haplotype analysis indicates that Omp lies proximal to sh-1.
Department of Biochemistry and Molecular Genetics, St. Mary's Hospital Medical School, London, United Kingdom.
K A
KA
Brown
M J
MJ
Sutcliffe
K P
KP
Steel
S D
SD
Brown
D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D003433:Crosses, Genetic; D003638:Deafness / Q000235:genetics*; D005260:Female; D008040:Genetic Linkage / Q000235:genetics*; D005914:Globins / Q000235:genetics; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D008969:Molecular Sequence Data; D014442:Monophenol Monooxygenase / Q000235:genetics; D009154:Mutation / Q000235:genetics; D009419:Nerve Tissue Proteins / Q000235:genetics*; D051842:Olfactory Marker Protein; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D015183:Restriction Mapping
D009419:Nerve Tissue Proteins; D051842:Olfactory Marker Protein; C497598:Omp protein, mouse; D005914:Globins; D014442:Monophenol Monooxygenase