Comparative evaluation of the colony-stimulating factors induction potential of Plasmodium cynomolgi-infected monkey erythrocytes and soluble antigens.
Plasmodium cynomolgi total antigens soluble in culture medium (P.c.SA), and noninfective P. cynomolgi-infected monkey erythrocytes (P.c.IE) were compared for their potential to induce colony-stimulating factors (CSFs). When injected intravenously in monkeys, both preparations induced an increase in the serum CSFs levels; P.c.IE appeared to be 1.6-fold more potent than the P.c.SA. In vitro P.c.IE induced 1.8-fold more CSF by monkey blood monocyte-derived macrophages than P.c. However, both in vivo and in vitro, the peak CSFs levels induced by P.c.SA were attained apparently 8 h earlier. CSF generated by P.c.SA and P.c.IE induced the formation of macrophage, granulocyte and granulocyte-macrophage colonies, in vitro; P.c.IE-generated CSF induced the formation of significantly (P < 0.01) higher numbers of granulocyte-macrophage colonies, indicating that the CSF induced by them stimulated different biological responses. The CSF induction appeared to be LPS-independent.
Singh
P P
PP
Division of Microbiology, Central Drug Research Institute, Lucknow, India.
Dutta
G P
GP
D000818:Animals; D000953:Antigens, Protozoan / Q000276:immunology*; D003115:Colony-Stimulating Factors / Q000096:biosynthesis*; D004912:Erythrocytes / Q000276:immunology / Q000469:parasitology; D005260:Female; D008253:Macaca mulatta; D008264:Macrophages / Q000276:immunology; D008288:Malaria / Q000276:immunology*; D008297:Male; D016789:Plasmodium cynomolgi / Q000276:immunology* / Q000302:isolation & purification; D012995:Solubility
D000953:Antigens, Protozoan; D003115:Colony-Stimulating Factors