Biochemical characterization of a variant form of cytosolic epoxide hydrolase induced by parental exposure to N-ethyl-N-nitrosourea.
1. ENU4 mice express a protein variant originally detected in a CBF1 mouse sired by a C57BL/6 mouse exposed to N-ethyl-N-nitrosourea. It appears to be an isoelectric point variant of cytosolic epoxide hydrolase. Affinity purified cytosolic epoxide hydrolase from ENU4 mice has a pI of approximately 5.1 compared to 5.6 in other mouse strains. 2. Clofibrate induced cytosolic epoxide hydrolase to similar levels in five strains of mice. However, CBF1 and ENU4 mice were more sensitive to the induction of palmitoyl CoA oxidase activity. 3. Except for isoelectric point, the physico- and immunochemical properties of cytosolic epoxide hydrolase from ENU4 mice were similar to those of the other mouse strains. Substrate specificities for five of six substrates tested were also similar.
Nourooz-Zadeh
J
J
Department of Entomology, University of California, Davis 95616.
Winder
B S
BS
Dietze
E C
EC
Giometti
C S
CS
Tollaksen
S L
SL
Hammock
B D
BD
D000818:Animals; D015153:Blotting, Western; D002846:Chromatography, Affinity; D002994:Clofibrate / Q000494:pharmacology; D003600:Cytosol / Q000201:enzymology*; D004591:Electrophoresis, Polyacrylamide Gel; D004790:Enzyme Induction; D004851:Epoxide Hydrolases / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D005038:Ethylnitrosourea / Q000633:toxicity*; D005260:Female; D007526:Isoelectric Point; D051379:Mice; D008807:Mice, Inbred BALB C; D010088:Oxidoreductases / Q000378:metabolism; D011247:Pregnancy
D010088:Oxidoreductases; C028427:palmitoyl CoA oxidase; D004851:Epoxide Hydrolases; D002994:Clofibrate; D005038:Ethylnitrosourea