[Study on P450c21 gene].
Yeast cells harboring mutant plasmids were incubated with 0.1, 0.15, 0.2, 0.3, 0.4, 0.45, 0.6, or 1.0 mM (14C)-17-hydroxyprogesterone for 1h. The steroids in the media were then separated by thin layer chromatography and counted to calculate enzymatic activities. Wt = wild type enzyme. Pt: patient enzyme.
B C
BC
Chung
D009154:Mutation; D013255:Steroid 21-Hydroxylase / Q000235:genetics*
D013255:Steroid 21-Hydroxylase