Structure of di-O-alpha-maltosyl cyclodextrins produced from alpha-maltosylfluoride and cyclodextrins.
The structures of di-O-alpha-maltosyl beta-cyclodextrins ((G2)2-beta-CDs), which were produced from alpha-maltosylfluoride (alpha-G2F) and cyclodextrin (CD) by the transfer action of debranching enzymes, were examined by the enzymic method using Bacillus subtilis saccharifying alpha-amylase (BSA). (G2)2-beta-CD was converted to (G1)2-beta-CD by treatment with glucoamylase before the examination. BSA completely hydrolyzed (G1)2-beta-CD to produce glucose, 6(3)-O-alpha-glucosylmaltotriose, and 6(3),6(5)-di-O-alpha-glucosyl maltopentaose. (G2)2-beta-CD was the mixture of 6A,6C-di-O-alpha-maltosyl beta-CD and 6A,6D-di-O-alpha-maltosyl beta-CD. The ratio of A,C/A,D in (G2)2-beta-CD synthesized with Pseudomonas isoamylase and Aerobacter pullulanase were 40:60-45:55 and 30:70, respectively. The content of 6A,6C-di-O-alpha-maltosyl gamma-CD in (G2)2-gamma-CD synthesized by isoamylase was about 35%.
Osaka Municipal Technical Research Institute, Japan.
Y
Y
Yoshimura
S
S
Kitahata
S
S
Okada
Y
Y
Satomura
K
K
Fujita
D001412:Bacillus subtilis / Q000201:enzymology; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D003505:Cyclodextrins / Q000096:biosynthesis / Q000737:chemistry* / Q000378:metabolism*; D004754:Enterobacter / Q000201:enzymology; D005087:Glucan 1,4-alpha-Glucosidase / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000378:metabolism; D007517:Isoamylase / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D008320:Maltose / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism; D008969:Molecular Sequence Data; D015394:Molecular Structure; D011549:Pseudomonas / Q000201:enzymology; D016339:Spectrometry, Mass, Fast Atom Bombardment; D000516:alpha-Amylases / Q000378:metabolism; D047392:beta-Cyclodextrins
D003505:Cyclodextrins; D047392:beta-Cyclodextrins; C068070:6,6-di-O-maltosyl-beta-cyclodextrin; C020395:alpha-maltosyl fluoride; D008320:Maltose; D006026:Glycoside Hydrolases; D000516:alpha-Amylases; D005087:Glucan 1,4-alpha-Glucosidase; C100181:pullulanase; C048745:cyclomaltodextrinase; D007517:Isoamylase