Expression of CD54, CD58, CD14, and HLA-DR on macrophages and macrophage-derived accessory cells and their accessory capacity.
Human peripheral monocytes can differentiate in vitro into macrophages (Mph) possessing a low accessory activity in T cell stimulation. Mph can be converted into a state of high accessory activity by treatment with dibutyryl cyclic AMP. This finding was used in this study to achieve Mph-derived AC (MphAC). Among the surface antigens on AC which have been shown to participate in accessory events leading to T cell proliferation, MHC class II antigens, CD58 (LFA-3) and CD54 (ICAM-1) seem to be especially important. We show here that the high accessory capacity of MphAC was not correlated with a high level of the surface antigens HLA-DR, CD58, and CD54. The amount of CD54 molecules was, in fact, lower on the MphAC than on the Mph.
Department of Zoophysiology, Uppsala University, Sweden.
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G
Ocklind
D
D
Friedrichs
J H
JH
Peters
D000911:Antibodies, Monoclonal; D000938:Antigen-Presenting Cells / Q000276:immunology*; D015703:Antigens, CD / Q000032:analysis*; D015214:Antigens, Differentiation, Myelomonocytic / Q000032:analysis; D018968:CD58 Antigens; D015815:Cell Adhesion Molecules / Q000032:analysis; D006684:HLA-DR Antigens / Q000032:analysis*; D006801:Humans; D018799:Intercellular Adhesion Molecule-1; D018950:Lipopolysaccharide Receptors; D008213:Lymphocyte Activation; D008264:Macrophages / Q000276:immunology*; D008934:Mitogens; D009000:Monocytes / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D018968:CD58 Antigens; D015815:Cell Adhesion Molecules; D006684:HLA-DR Antigens; D018950:Lipopolysaccharide Receptors; D008934:Mitogens; D018799:Intercellular Adhesion Molecule-1