Practical considerations in the measurement of culture fluorescence.
We have examined practical considerations associated with the use of a commercially available fluorescence probe for in situ measurements in bioreactors. The optical path length of the measurement was first determined and a flow cell subsequently designed. The environment (agitation/aeration rates) of the probe was found to have a significant influence on the measurement. These effects were eliminated by placing the probe in a recycle loop using the flow cell. Fluorescence measurement in the recycle loop was verified to be representative of the cell sample and to not affect cell metabolism.
Maryland Biotechnology Institute, University of Maryland Baltimore County 21228.
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Coppella
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Rao
D004926:Escherichia coli / Q000166:cytology* / Q000378:metabolism; D005285:Fermentation; D005453:Fluorescence; D005456:Fluorescent Dyes; D009249:NADP / Q000378:metabolism
D005456:Fluorescent Dyes; D009249:NADP