Sandwich capture ELISA by a murine monoclonal antibody against a genus-specific LPS epitope for the detection of different common serotypes of salmonellas.
A sandwich capture ELISA based on a murine monoclonal antibody against a genus-specific epitope in the outer core region of the Salmonella lipopolysaccharide is described for the detection of different common serotypes of salmonellas. Four h broth cultures of seven standard and 24 wild strains of salmonellas were all detected by the capture ELISA while overnight broth cultures of 21 non-salmonella standard strains were all negative. The capture ELISA detected 1 ng/ml of Ra lipopolysaccharide, 10(6)/ml of a smooth wild strain of Salm. typhimurium, and 1120 cells of Salm. heidelberg after enrichment culture for 4 h.
Department of Microbiology, University of Hong Kong, Queen Mary Hospital Compound.
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D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D001419:Bacteria / Q000276:immunology / Q000302:isolation & purification; D004306:Dose-Response Relationship, Immunologic; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology; D008070:Lipopolysaccharides / Q000276:immunology*; D051379:Mice; D011237:Predictive Value of Tests; D015203:Reproducibility of Results; D012475:Salmonella / Q000145:classification / Q000276:immunology / Q000302:isolation & purification*; D012703:Serotyping
D000911:Antibodies, Monoclonal; D000939:Epitopes; D008070:Lipopolysaccharides