Enkephalin hydrolysis by mouse plasma in vitro.
Hydrolysis of [Leu]- and [Met]enkephalin was determined in samples of pooled whole mouse plasma in vitro by using HPLC-ECD to measure accumulation of Tyr-containing metabolites. More Tyr-Gly-Gly accumulated from [Met]enkephalin than from [Leu]enkephalin hydrolysis, and [Met]enkephalin's half-life in mouse plasma was approximately half that of [Leu]enkephalin. Comparisons of metabolite formation in the presence versus the absence of inhibitors with high selectivity for various peptidases demonstrated that a bestatin-sensitive aminopeptidase, presumably aminopeptidase M, as well as enkephalinase and angiotensin converting enzyme, participate in the hydrolysis of enkephalin in mouse plasma.
Shibanoki
S
S
Department of Pharmacology, Nihon University School of Medicine, Tokyo, Japan.
Weinberger
S B
SB
Schulteis
G
G
Ishikawa
K
K
Martinez
J L
JL
D000595:Amino Acid Sequence; D000626:Aminopeptidases / Q000097:blood; D000818:Animals; D018826:CD13 Antigens; D002851:Chromatography, High Pressure Liquid; D004563:Electrochemistry; D010450:Endopeptidases / Q000097:blood; D004743:Enkephalin, Leucine / Q000097:blood*; D004744:Enkephalin, Methionine / Q000097:blood*; D006868:Hydrolysis; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D015260:Neprilysin / Q000097:blood; D007703:Peptidyl-Dipeptidase A / Q000097:blood; D010949:Plasma / Q000201:enzymology; D013997:Time Factors
D004744:Enkephalin, Methionine; D004743:Enkephalin, Leucine; D010450:Endopeptidases; D000626:Aminopeptidases; D018826:CD13 Antigens; C020071:dipeptidyl carboxypeptidase; D007703:Peptidyl-Dipeptidase A; D015260:Neprilysin