P-glycoprotein genes of Entamoeba histolytica.
Six different P-glycoprotein gene segments were identified from an emetine-resistant E. histolytica mutant, which overexpresses mRNAs homologous to segments of the human mdr1 (P-glycoprotein) gene. The open reading frames of two completely sequenced genes EhPgp1 and EhPgp2 were 1,302 and 1,310 amino acids long, respectively, and showed a 67% positional identity with each other and 41 and 40% positional identities, respectively, with human mdr1 gene. Within each ameba P-glycoprotein were the ATP-binding sites found twice in eukaryotic P-glycoproteins and once in prokaryotic transport proteins. A phylogenetic tree showed that Entamoeba P-glycoproteins are more related to the human and mouse P-glycoproteins than to the Plasmodium and Leishmania P-glycoproteins. In addition, there were two P-glycoprotein pseudogenes, each with a frame shift and stop codons in identical places within the amino ATP-binding site.
Department of Tropical Public Health, Harvard School of Public Health, Boston, MA.
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Descoteaux
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Shen
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Ayala
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Orozco
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Samuelson
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics*; D016054:DNA, Protozoan / Q000235:genetics*; D004748:Entamoeba histolytica / Q000235:genetics*; D017125:Genes, Protozoan; D006801:Humans; D007891:Leishmania / Q000235:genetics; D008562:Membrane Glycoproteins / Q000235:genetics*; D051379:Mice / Q000235:genetics; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D010963:Plasmodium falciparum / Q000235:genetics; D015800:Protozoan Proteins / Q000235:genetics*; D011544:Pseudogenes; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D002352:Carrier Proteins; D016054:DNA, Protozoan; D008562:Membrane Glycoproteins; D015800:Protozoan Proteins