Degradation of isomeric monochlorobenzoates and 2,4-dichlorophenoxyacetic acid by a constructed Pseudomonas sp.
A 4-chlorobenzoate-degrading Pseudomonas sp. US1 was mated with a strain of Escherichia coli JMP 397 (harbouring the plasmid pJP4). An ex-conjugant designated Pseudomonas sp. US1 ex that could utilize all the isomeric monochlorobenzoates and 2,4-dichlorophenoxyacetate was obtained. The ex-conjugant released stoichiometric amounts of chloride when grown on these chloroaromatics as sole sources of carbon and energy.
Department of Microbiology and Biotechnology Centre, Faculty of Science, M.S. University of Baroda, India.
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Sahasrabudhe
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Modi
D015084:2,4-Dichlorophenoxyacetic Acid / Q000378:metabolism*; D002723:Chlorobenzoates / Q000378:metabolism*; D005818:Genetic Engineering; D006868:Hydrolysis; D010957:Plasmids; D011549:Pseudomonas / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism
D002723:Chlorobenzoates; D015084:2,4-Dichlorophenoxyacetic Acid