Identification and nucleotide sequence of the leukocyte and reticulocyte forms of rabbit cytochrome b5 mRNA.
RNA extracted from rabbit leukocytes and reticulocytes was reverse transcribed and used in the Polymerase Chain Reaction technique along with primers designed to amplify the coding sequence of rabbit cytochrome b5. The resultant amplified products were subcloned and analyzed. Sequencing confirmed that leukocyte and liver cDNAs are homologous and encode the membrane-bound form of the protein. In contrast, reticulocytes exhibit a highly similar, but different mRNA which encodes the smaller, soluble cytochrome b5. This is the first example of a cytochrome b5 sequence from a tissue other than liver, erythrocyte or reticulocyte.
Department of Biochemistry, Northeastern Ohio Universities College of Medicine, Rootstown 44272.
S J
SJ
Giordano
A W
AW
Steggles
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015786:Cytochromes b5 / Q000737:chemistry / Q000235:genetics*; D007962:Leukocytes / Q000737:chemistry*; D008099:Liver / Q000737:chemistry; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000737:chemistry*; D011817:Rabbits; D012156:Reticulocytes / Q000737:chemistry*; D012689:Sequence Homology, Nucleic Acid
D012333:RNA, Messenger; D015786:Cytochromes b5