An acetate-sensitive mutant of Neurospora crassa deficient in acetyl-CoA hydrolase.
The predicted amino acid sequence of the product of the acetate-inducible acu-8 gene of Neurospora crassa, previously of unknown function, has close homology to the recently published sequence of Saccharomyces cerevisiae acetyl-CoA hydrolase. An acu-8 mutant strain, previously characterized as acetate non-utilizing, shows strong growth-inhibition by acetate, but will use it as carbon source at low concentrations. The mutant was shown to be deficient in acetyl-CoA hydrolase and to accumulate acetyl-CoA when supplied with acetate. As in Saccharomyces, the Neurospora enzyme is acetate-inducible.
Protein Engineering Department, AFRC Institute of Food Research, Reading, UK.
I F
IF
Connerton
W
W
McCullough
J R
JR
Fincham
D000085:Acetates / Q000494:pharmacology*; D000104:Acetyl-CoA Hydrolase / Q000172:deficiency* / Q000235:genetics; D000595:Amino Acid Sequence; D008969:Molecular Sequence Data; D009154:Mutation; D009492:Neurospora crassa / Q000187:drug effects* / Q000201:enzymology* / Q000254:growth & development
D000085:Acetates; D000104:Acetyl-CoA Hydrolase