Evolution of the retrotransposons TRS/ingi and of the tubulin genes in trypanosomes.
The African trypanosomes have genomes of high plasticity, as demonstrated for instance by their ability to shuffle their genes around, coding for variant-specific surface glycoproteins (VSGs). Another indication of their genome plasticity is the presence of multiple retro-elements. The retrotransposon-like element TRS/ingi is present in many copies in the genome of trypanosomes. One particular derivative of TRS/ingi, called TUBIS, had previously been found to interrupt a tubulin gene in a particular strain of T. brucei. Here both TRS/ingi and TUBIS were studied by hybridizing genomic DNA of various strains and species of trypanosomes with suitable probes in order to elucidate the evolution of this family of retro-elements. The TSR/ingi elements are highly repeated and have very long open reading frames, while TUBIS clearly is a truncated, inactivated form of this element, found in only one particular chromosomal location. Both elements were shown to be present in several strains and species of the subgenus Trypanozoon, in particular in T. brucei brucei, T. gambiense, T. rhodesiense, T. equiperdum and T. evansi. They could not be detected in species of other subgenera, in particular in T. congolense and T. cruzi. These findings suggest that the retrotransposon TRS/ingi was acquired by trypanosomes only after divergence of present day subgenera. The TUBIS element was found in exactly the same chromosomal location (at the 3' end of the tubulin gene cluster) in many different strains and species of the subgenus Trypanozoon. This shows that the element was transposed to this location before speciation of the subgenus. Although, TRS/ingi is unlikely to be involved directly in VSG switching, it may have contributed to the genome plasticity of trypanosomes.
Institute for General Microbiology, University of Bern, Switzerland.
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Braun
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Behrens
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Glauser
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Brun
D000818:Animals; D004251:DNA Transposable Elements / Q000235:genetics*; D017125:Genes, Protozoan; D005810:Multigene Family; D010957:Plasmids / Q000235:genetics; D014345:Trypanosoma / Q000145:classification / Q000235:genetics* / Q000254:growth & development; D014346:Trypanosoma brucei brucei / Q000235:genetics; D014404:Tubulin / Q000235:genetics*
D004251:DNA Transposable Elements; D014404:Tubulin