Isolation of vindoline from Catharanthus roseus by supercritical fluid extraction.
Vindoline was extracted from the leaves of Catharanthus roseus over the ranges of 35-70 degrees C and 100-300 bar using supercritical carbon dioxide with and without the addition of 3 wt % ethanol as a cosolvent. The vindoline contents in the extracts were determined by HPLC and identified by LC/MS. The remarkable highest vindoline concentration, 58 wt %, was obtained at the lowest temperature, 35 degrees C, and the highest pressure, 300 bar, of this study. The use of a cosolvent only slightly improved the extraction yields or selectivities at some experimental conditions.
Department of Chemical Engineering, Korea Advanced Institute of Science and Technology, Daejon.
K M
KM
Song
S W
SW
Park
W H
WH
Hong
H
H
Lee
S S
SS
Kwak
J R
JR
Liu
D001709:Biotechnology; D002245:Carbon Dioxide; D000431:Ethanol; D005069:Evaluation Studies as Topic; D010946:Plants, Medicinal / Q000737:chemistry*; D011312:Pressure; D012997:Solvents; D013696:Temperature; D014747:Vinblastine / Q000031:analogs & derivatives* / Q000302:isolation & purification
D012997:Solvents; D002245:Carbon Dioxide; D000431:Ethanol; C009667:vindoline; D014747:Vinblastine