ELISA and indirect immunofluorescence in the diagnosis of LCM virus infections.
Thirty-seven matched samples of patient sera with the clinical diagnosis of a lymphocytic choriomeningitis (LCM) infection, as well as 56 matched samples of patient sera with the clinical diagnosis of a CNS infection of vague etiology were examined. Two serological techniques, indirect immunofluorescence (IF) and ELISA were used. They revealed 16.2% of positive sera confirming the clinical diagnosis of the disease; in the cases of clinical diagnosis of CNS infection of vague etiology 8.9% of positive sera were found, which points to an LCM virus-caused infection.
Public Health Institute of the Republic of Croatia, Zagreb.
B
B
Turković
M
M
Ljubicić
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D008216:Lymphocytic Choriomeningitis / Q000175:diagnosis*; D008217:Lymphocytic choriomeningitis virus / Q000276:immunology*; D051379:Mice; D012680:Sensitivity and Specificity; D015047:Zoonoses
D000914:Antibodies, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M