High-performance liquid chromatographic determination of floctafenine and its major metabolite, floctafenic acid in plasma.
A simple, rapid and selective high-performance liquid chromatographic method for the determination of floctafenine and its major metabolite, floctafenic acid, in plasma is reported. Plasma samples were purified using the mobile phase as a protein precipitant. The supernatant containing parent compounds and diazepam (internal standard) were eluted from a 5 micron C 18 reversed-phase column at ambient temperature. The mobile phase consisted of 0.05 M sodium acetate:acetonitrile: methanol (200:100:100 v/v/v) adjusted to pH 5 and pumped isocratically at a flow rate of 1.5 ml/min. The effluent was monitored at 350 nm. Quantification was achieved by the measurement of the peak-height ratio of each drug to the internal standard. The mean percentage recoveries from plasma samples spiked with floctafenine and floctafenic acid ranged from 88.13 to 101.93%. Detection limits were 100 ng/ml for floctafenine and 50 ng/ml for floctafenic acid. The coefficients of variation (RSD, %) for within-day and day-to-day analysis were less than 8%.
Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Alexandria University, Egypt.
M H
MH
Abdel-Hay
A M
AM
Gharaibeh
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D062367:ortho-Aminobenzoates / Q000031:analogs & derivatives / Q000097:blood*
D062367:ortho-Aminobenzoates