Coiled tube membrane bioreactor for cultivation of hybridoma cells producing monoclonal antibodies.
A coiled tube membrane reactor was developed for the cultivation of mouse-mouse hybridoma cells producing monoclonal antibodies. The cell and antibody concentrations obtained in the membrane reactor were higher than that obtained in a batch spinner flask without a membrane. A mathematical model has been developed to describe the membrane transport coupled growth and product formation, and the physical and kinetic constants of the system were determined.
Department of Chemical Engineering, Washington University, St. Louis, Missouri 63130.
J
J
Hagedorn
F
F
Kargi
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D002455:Cell Division; D002460:Cell Line; D002470:Cell Survival; D046508:Culture Techniques / Q000295:instrumentation* / Q000379:methods; D006825:Hybridomas / Q000166:cytology* / Q000276:immunology; D007700:Kinetics; D051379:Mice; D008954:Models, Biological
D000911:Antibodies, Monoclonal