Differential expression of the neu oncogene in mouse liver and pancreatic cell lines.
To study the tissue-specific expression of neu oncogene, we used two mouse tumor cell lines derived from liver, Hep1-a, and pancreatic, 266-6, tumors as a model system. The endogenous neu gene is expressed in Hep1-a but not in 266-6 cells. We demonstrate in this report that differential expression of the neu gene in these two cell lines is mainly regulated at transcriptional level. The neu promoter sequence responsible for the differential regulation is localized within a 90 bp region and it is possibly due to lack of a specific positive transcription factor(s) interacting with this region in 266-6 cells.
Department of Tumor Biology, University of Texas, M. D. Anderson Cancer Center, Houston 77030.
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Yan
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Marin
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Hung
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D015870:Gene Expression; D015972:Gene Expression Regulation, Neoplastic; D008114:Liver Neoplasms, Experimental / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D010190:Pancreatic Neoplasms / Q000235:genetics*; D010957:Plasmids; D011401:Promoter Regions, Genetic; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D011519:Proto-Oncogenes; D018719:Erb-b2 Receptor Tyrosine Kinases; D015183:Restriction Mapping
D009838:Oligodeoxyribonucleotides; D011518:Proto-Oncogene Proteins; D018719:Erb-b2 Receptor Tyrosine Kinases