Regulation of biosynthesis of bacilysin by Bacillus subtilis.
Production of the dipeptide antibiotic bacilysin by Bacillus subtilis 168 was growth associated and showed no evidence of repression by glucose or sucrose. Carbohydrates other than glucose and sucrose yielded lower specific titers of bacilysin. Bacilysin production in three such carbon sources (maltose, xylose, ribose) was delayed until growth slowed down. Ammonium salts were poor for bacilysin production when used as the sole nitrogen source. When added to the standard medium containing glutamate, they suppressed antibiotic production. Aspartate was slightly better than glutamate for antibiotic production as sole nitrogen source. No other nitrogen source tested, including inorganic, organic or complex, approached the activity of glutamate or aspartate. When added to glutamate, casamino acids, phenylalanine and alanine (a substrate of bacilysin synthetase) suppressed bacilysin production while stimulating growth. Phosphate provided for optimum growth and production at 7.5 mM and both processes were inhibited at higher concentrations. Ferric citrate stimulated growth and inhibited bacilysin production, the effects being due to both the iron and the citrate components. Elimination of ferric citrate stimulated production as did increasing the concentration of Mn to its optimum concentration of 6.6 x 10(-4) M.
Biology Department, Middle East Technical University, Ankara, Turkey.
G
G
Ozcengiz
N G
NG
Alaeddinoglu
A L
AL
Demain
D000596:Amino Acids / Q000378:metabolism; D000645:Ammonium Sulfate / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D001412:Bacillus subtilis / Q000254:growth & development / Q000378:metabolism*; D050260:Carbohydrate Metabolism; D003470:Culture Media; D004151:Dipeptides / Q000096:biosynthesis; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D005971:Glutamates / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D009584:Nitrogen / Q000378:metabolism*; D010710:Phosphates / Q000378:metabolism; D013395:Sucrose / Q000378:metabolism; D014131:Trace Elements / Q000378:metabolism
D000596:Amino Acids; D000900:Anti-Bacterial Agents; D003470:Culture Media; D004151:Dipeptides; D005971:Glutamates; D010710:Phosphates; D014131:Trace Elements; C017721:casamino acids; D013395:Sucrose; C006674:bacilysin; D005947:Glucose; D009584:Nitrogen; D000645:Ammonium Sulfate