Properties of a cell line (MEG-01SSF) of human megakaryoblastic leukemia cells.
MEG-01SSF cells were prepared from MEG-01S by conditioning in a chemically defined, serum-free medium. The cells grew well in the medium and required transferrin for growth. Doubling time was about 20 hours. The cells retained GpIIb/IIIa as a marker of megakaryocytes and transferrin receptors on the cell surface. Some of the cells in the stationary phase of growth produced platelet-like particles, which bore characteristic microtubule rings.
Aichi Cancer Center Hospital, Nagoya.
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Takeuchi
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Satoh
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Ogura
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Saito
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Takeuchi
D000954:Antigens, Surface / Q000032:analysis; D001792:Blood Platelets / Q000166:cytology*; D002454:Cell Differentiation / Q000502:physiology; D005455:Fluorescent Antibody Technique; D006801:Humans; D007947:Leukemia, Megakaryoblastic, Acute / Q000473:pathology*; D010980:Platelet Membrane Glycoproteins / Q000032:analysis; D011990:Receptors, Transferrin / Q000032:analysis; D014404:Tubulin / Q000276:immunology; D014407:Tumor Cells, Cultured
D000954:Antigens, Surface; D010980:Platelet Membrane Glycoproteins; D011990:Receptors, Transferrin; D014404:Tubulin