Cloning and sequencing of the xynA gene encoding xylanase A of Aspergillus kawachii.
We have cloned the xynA gene coding for xylanase A, a major component of the xylanase family, from Aspergillus kawachii. The cDNA was isolated from an A. kawachii cDNA library by immunoscreening using antibody raised against the purified xylanase A protein. Nucleotide sequence analysis of the cDNA showed a 981-bp open reading frame that encoded a protein of 327 amino acid residues. The signal peptide was composed of 25 amino acid residues and the N-terminus of the mature protein was pyroglutamic acid. The transformed yeast with a cloned cDNA produced xylanase. The genomic DNA was arranged as ten exons and nine introns.
National Research Institute of Brewing, Tokyo, Japan.
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Ito
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Ikemasu
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Ishikawa
D000595:Amino Acid Sequence; D001230:Aspergillus / Q000201:enzymology / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D003062:Codon / Q000235:genetics; D043364:Endo-1,4-beta Xylanases; D004926:Escherichia coli / Q000235:genetics; D015723:Gene Library; D005800:Genes, Fungal; D006026:Glycoside Hydrolases / Q000235:genetics*; D007438:Introns; D008969:Molecular Sequence Data; D012326:RNA Splicing; D012331:RNA, Fungal / Q000235:genetics; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid
D003062:Codon; D012331:RNA, Fungal; D006026:Glycoside Hydrolases; D043364:Endo-1,4-beta Xylanases