[A structural protein study of the influenza A (H1N1) virus by polyacrylamide gel electrophoresis].
Influenza is an acute respiratory disease typically appearing as an epidemic. Three immunological types of the influenza virus are known: A, B and C. Continually, antigen changes occur, especially in type A. Therefore, a comparative study was carried out on 4 influenza A(H1N1) virus strains in relation to protein structure (surface antigens), by using polyacrylamide gel electrophoresis by the modified Laemmli method. The objective was to compare the structural proteins of the A/Havana/1292/78 (H1N1) national strain with the proteins of 3 international pattern strains. In all the cases, 6 bands were detected by densitometry. In the 4 strains studied the most abundant protein was M. Great differences between the Cuban strain and the 3 international patterns were not seen.
Pérez Guevara
M T
MT
Instituto Nacional de Higiene, Epidemiología y Microbiología.
Savón Valdés
C
C
Rivas Arjona
M
M
Goyenechea Hernández
A
A
D000956:Antigens, Viral / Q000032:analysis; D003462:Cuba; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D053118:Influenza A Virus, H1N1 Subtype; D009980:Influenza A virus / Q000737:chemistry* / Q000276:immunology; D015678:Viral Structural Proteins / Q000032:analysis*
D000956:Antigens, Viral; D015678:Viral Structural Proteins
Estudio de las proteínas estructurales de influenzavirus A (H1N1) por electroforesis en gel de poliacrilamida.