Shear thickening of DNA in SDS lysates.
Cells of Escherichia coli were subjected to lysis by an alkali detergent treatment (sodium dodecyl sulphate). The rate of detergent lysis was not first order. The rheological properties of the lysate were measured using a controlled stress rheometer. Detergent-lysed cells produced a lysate which showed marked non-Newtonian properties. The material showed a shear thickening at specific low shear stress conditions. The acceleration of the cone during the ascent stage of a flow curve, influenced the shape of the flow curves. These findings are discussed in relation to the form of bacterial DNA in solution.
Stephenson
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Division of Chemical and Biotechnological Sciences, School of Science and Technology, University of Teesside, Middlesborough, Cleveland, UK.
Norman
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Cumming
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RH
D004269:DNA, Bacterial / Q000737:chemistry*; D004926:Escherichia coli / Q000737:chemistry; D006868:Hydrolysis; D012212:Rheology; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry*
D004269:DNA, Bacterial; D012967:Sodium Dodecyl Sulfate