Role and regulation of interleukin (IL)-2 receptor alpha and beta chains in IL-2-driven B-cell growth.
Substantial proportions of resting B cells constitutively express low levels of IL-2 receptor (IL-2R) alpha and/or beta chains. The expression of these chains is differentially regulated by anti-IgM and IL-2/IL-4. The anti-IgM induces IL-2R alpha chain expression, whereas each of the two cytokines induces IL-2R beta chain expression in a dose-dependent manner. Moreover, IL-2 induces the growth of B cells, when the cells were pretreated with IL-2 or IL-4 for 24 h. The magnitude of this IL-2-driven B-cell growth depends upon the level of IL-2R beta chain expression. Costimulation of the B cells with IL-2 and anti-IgM shifts the dose-response curve, and the cells proliferate at an IL-2 concentration as low as 40 pM. These results indicate that the levels of anti-IgM-induced IL-2R alpha chain and IL-2-induced IL-2R beta chain determine the sensitivity of the cells to IL-2.
Third Department of Internal Medicine, Hyogo College of Medicine, Japan.
K
K
Nakanishi
S
S
Hirose
T
T
Yoshimoto
H
H
Ishizashi
K
K
Hiroishi
T
T
Tanaka
T
T
Kono
M
M
Miyasaka
T
T
Taniguchi
K
K
Higashino
D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000276:immunology*; D002478:Cells, Cultured; D015342:DNA Probes; D004261:DNA Replication; D005260:Female; D005455:Fluorescent Antibody Technique; D015870:Gene Expression / Q000187:drug effects; D007075:Immunoglobulin M / Q000276:immunology; D007376:Interleukin-2 / Q000494:pharmacology*; D015847:Interleukin-4 / Q000494:pharmacology; D007700:Kinetics; D008213:Lymphocyte Activation / Q000187:drug effects; D046911:Macromolecular Substances; D051379:Mice; D008807:Mice, Inbred BALB C; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D015375:Receptors, Interleukin-2 / Q000235:genetics / Q000502:physiology*; D013997:Time Factors
D015342:DNA Probes; D007075:Immunoglobulin M; D007376:Interleukin-2; D046911:Macromolecular Substances; D012333:RNA, Messenger; D015375:Receptors, Interleukin-2; D015847:Interleukin-4; D012313:RNA