Increased expression of retroviral sequences in progressionally advanced rat prostatic tumors.
Differential hybridization analysis revealed two cDNA clones, pBUS19 and pBUS30, to be overexpressed in progressionally advanced rat prostatic tumors. Northern blot analysis suggested the clones to be related although no homology in nucleotide sequence could be shown. Isolation and characterization of a pBUS19-related clone, pJG116, and computer-assisted database comparison showed that all three clones could be mapped within a rat-specific endogenous retrovirus. The importance of overexpression of retroviral sequences in advanced prostatic cancer remains unclear.
Department of Urology (Urological Research Laboratory), University of Nijmegen, The Netherlands.
M J
MJ
Bussemakers
A
A
van Bokhoven
J J
JJ
van Groningen
F M
FM
Debruyne
J A
JA
Schalken
D000818:Animals; D015152:Blotting, Northern; D004273:DNA, Neoplasm / Q000235:genetics / Q000302:isolation & purification; D004279:DNA, Viral / Q000235:genetics* / Q000302:isolation & purification; D015723:Gene Library; D008297:Male; D008969:Molecular Sequence Data; D010957:Plasmids; D011467:Prostate / Q000502:physiology; D011471:Prostatic Neoplasms / Q000235:genetics / Q000382:microbiology* / Q000473:pathology; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012334:RNA, Neoplasm / Q000235:genetics / Q000302:isolation & purification; D051381:Rats; D012190:Retroviridae / Q000235:genetics* / Q000302:isolation & purification
D004273:DNA, Neoplasm; D004279:DNA, Viral; D012334:RNA, Neoplasm; D012313:RNA