In vivo and in vitro protein synthesis of oviducal pars recta by estradiol effect.
1. Proteins secreted by toad oviductal pars recta are involved in fertilization and their biological activity is regulated by estrogens. 2. Effect of 17 beta-estradiol on protein synthesis was examined on castrated animals by different in vivo and in vitro experimental approaches. 3. Different routes of L-[3H]leucine administration were assessed. An intralymphatic route was the most efficient for incorporating radioactivity per mg of trichloroacetic acid insoluble proteins. 4. Ligature of pars recta induces protein synthesis at a similar level to exogenous estradiol. 5. Electrophoretic pattern of radioactive proteins did not show synthesis of a specific protein related to the zone with biological activity. 6. Pars recta releases newly synthesized proteins in vivo into its fluid secretion as much as in vitro into culture medium.
Mansilla-Whitacre
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ZC
Departamento de Biología del Desarrollo, INSIBIO, CONICET-Universidad Nacional de Tucumán, Argentina.
Fernández
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SN
Miceli
D C
DC
D000818:Animals; D002022:Bufo arenarum; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D066298:In Vitro Techniques; D007930:Leucine / Q000008:administration & dosage / Q000378:metabolism; D010052:Ovariectomy; D010057:Oviducts / Q000187:drug effects* / Q000293:injuries / Q000502:physiology; D014176:Protein Biosynthesis; D011506:Proteins / Q000378:metabolism
D011506:Proteins; D004958:Estradiol; D007930:Leucine