Characterization of cholinesterases from the parasitic nematode Trichinella spiralis.
1. Trichinella cholinesterases occur in multiple molecular forms which differ in size, kinetics, activity with butyrylthiocholine, and effects of inhibitors. 2. The 5.3 and 13S forms identified in Trichinella extracts are also found in C. elegans and other nematodes but the 7S form which occurs in other nematodes was absent from Trichinella detergent extracts. Differences in kinetic and inhibition properties among nematode species were also evident. 3. The level of cholinesterases in excretory/secretory products is low. 4. Trichinella cholinesterases did not elicit a detectable antibody response in mice.
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Department of Zoology, University of Manitoba, Winnipeg, Canada.
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D000818:Animals; D000909:Antibodies, Helminth / Q000032:analysis; D002093:Benzenaminium, 4,4'-(3-oxo-1,5-pentanediyl)bis(N,N-dimethyl-N-2-propenyl-), Dibromide / Q000494:pharmacology; D002800:Cholinesterase Inhibitors / Q000494:pharmacology*; D002802:Cholinesterases / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D051379:Mice; D010830:Physostigmine / Q000494:pharmacology; D013770:Tetraisopropylpyrophosphamide / Q000494:pharmacology; D017160:Trichinella spiralis / Q000201:enzymology*; D014235:Trichinellosis / Q000276:immunology
D000909:Antibodies, Helminth; D002800:Cholinesterase Inhibitors; D002093:Benzenaminium, 4,4'-(3-oxo-1,5-pentanediyl)bis(N,N-dimethyl-N-2-propenyl-), Dibromide; D013770:Tetraisopropylpyrophosphamide; D010830:Physostigmine; D002802:Cholinesterases