High efficiency electroporation of Lactococcus lactis subsp. lactis LM0230 with plasmid pGB301.
Electroporation-mediated transformation of Lactococcus lactis with plasmid pGB301, a 9.8 kilobase pair vector (Behnke et al. 1981), has been reported by McIntyre & Harlander (1989a). Improved transformation efficiencies of 10(2)-10(3)/micrograms DNA were achieved by altering the conditions under which the bacteria were grown prior to electroporation (McIntyre & Harlander 1989b). This present investigation sought to improve still further transformation efficiencies in order to provide a reliable high frequency transformation system for Lc. lactis subsp. lactis.
Dornan
S
S
Department of Food and Agricultural Microbiology, Queen's University of Belfast, Northern Ireland.
Collins
M A
MA
D004269:DNA, Bacterial / Q000235:genetics*; D005822:Genetic Vectors; D013294:Lactococcus lactis / Q000235:genetics*; D010957:Plasmids; D014169:Transformation, Bacterial
D004269:DNA, Bacterial