Interactions of cells from peritoneal dialysate with mesothelial cells and fibroblasts in culture.
Peritoneal mesothelial cells and fibroblasts were co-cultured in vitro with peritoneal white blood cells (PWBC) obtained from CAPD patients, after an overnight exchange with 0.5% Dianeal or 2.5% Dianeal. Unstimulated PWBC inhibited proliferation of mesothelial cells and fibroblasts. Upon stimulation with lipoposaccharides (LPS), PWBC from the 0.5% dextrose exchange, enhanced the growth of mesothelial cells and fibroblasts, whereas when stimulated with LPS, PBWC from the 2.5% dextrose exchange increased only proliferation of fibroblasts.
Breborowicz
A
A
Division of Nephrology, University of Toronto, Canada.
Balaskas
E
E
Oreopoulos
G D
GD
Vas
M
M
Martis
L
L
Serkes
K D
KD
Oreopoulos
D G
DG
D002455:Cell Division; D002478:Cells, Cultured; D015314:Dialysis Solutions; D004847:Epithelial Cells; D005347:Fibroblasts / Q000166:cytology*; D006801:Humans; D007962:Leukocytes / Q000166:cytology / Q000502:physiology*; D009852:Omentum / Q000033:anatomy & histology; D010529:Peritoneal Cavity / Q000166:cytology*; D010531:Peritoneal Dialysis, Continuous Ambulatory
D015314:Dialysis Solutions