Structure of rice-straw arabinoglucuronoxylan and specificity of Streptomyces xylanase toward the xylan.
Rice-straw treated with 5% ammonia water was hydrolyzed with the beta-xylanase of Streptomyces sp. E-86, and four kinds of hetero-oligosaccharides, two arabinoxylo- and two glucuronoxylo-oligosaccharides, were isolated from the hydrolysate by charcoal column, gel filtration, ion-exchange, and aminopropyl silica column chromatographies. The structures of the oligosaccharides were identified as 3(2)-alpha-L-arabinofuranosyl-xylobiose, 3(2)-alpha-L-arabinofuranosylxylotriose, 2(3)-4-O-methyl-alpha-D-glucuronosylxylotriose, and 2(3)-alpha-D-glucuronosylxylotriose by the analysis of component sugar, partial acid hydrolysis, methylation analysis etc. Based on the structures of above oligosaccharides, the structure of rice-straw arabinoglucuronoxylan and the specificity of the xylanase toward the xylan are discussed.
Yoshida
S
S
Institute of Applied Biochemistry, University of Tsukuba, Ibaraki, Japan.
Kusakabe
I
I
Matsuo
N
N
Shimizu
K
K
Yasui
T
T
Murakami
K
K
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D043364:Endo-1,4-beta Xylanases; D006026:Glycoside Hydrolases / Q000378:metabolism*; D006868:Hydrolysis; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000302:isolation & purification; D012275:Oryza; D011134:Polysaccharides; D013302:Streptomyces / Q000201:enzymology*; D013379:Substrate Specificity; D014990:Xylans
D009844:Oligosaccharides; D011134:Polysaccharides; D014990:Xylans; D006026:Glycoside Hydrolases; D043364:Endo-1,4-beta Xylanases