High density culture of anchorage-dependent animal cells by polyurethane foam packed-bed culture systems.
Monkey kidney cells (Vero) and Chinese hamster ovary cells (CHO-K1) attached to the internal surface of polyurethane foam (PUF) and grew to a high cell density (1.1 x 10(8) cells/cm3 PUF and 4.2 x 10(7) cells/cm3 PUF, respectively) in a PUF-plates packed-bed culture system. This density of Vero cells was twice that obtained previously with a PUF-particles packed-bed culture system. A maximum cell density of 6.7 x 10(7) cells/cm3 culture vessel volume was obtained in a PUF-disc packed-bed culture of Vero cells. From the cell density of CHO-K1, growing in a monolayer on the surface of PUF and a petri dish, per bulk volume of PUF, we estimated that a surface area to volume ratio of PUF plates effective for cell growth was about 109 cm2/cm3.
Department of Chemical Engineering, Faculty of Engineering, Kyushu University, Fukuoka, Japan.
T
T
Matsushita
H
H
Hidaka
K
K
Kamihata
Y
Y
Kawakubo
K
K
Funatsu
D000818:Animals; D001709:Biotechnology / Q000295:instrumentation*; D002448:Cell Adhesion; D002452:Cell Count; D002522:Chlorocebus aethiops; D006224:Cricetinae; D003412:Cricetulus; D003470:Culture Media; D005260:Female; D010053:Ovary; D011140:Polyurethanes; D014709:Vero Cells
D003470:Culture Media; D011140:Polyurethanes