Protease-catalysed peptide synthesis in aqueous-organic two-phase systems: reactant precipitation and interfacial inactivation.
Peptides can be efficiently synthesized by the proteases thermolysin and pepsin acting in aqueous-organic two-phase systems similar to those reported previously. However, a number of problems can prevent successful reactions in these systems, with these and other proteases: (1) Complexes between amino acid reactants that are sparingly soluble in both phases may be precipitated, though their solubility behavior is complex; (2) the enzyme may be subject to rapid inactivation by contact with the phase interface, and the rate of this is unusually dependent on the nature of the solvent; and (3) the reaction rate is usually slow.
Department of Bioscience & Biotechnology, University of Strathclyde, Glasgow, UK.
J M
JM
Cassells
P J
PJ
Halling
D000596:Amino Acids; D010450:Endopeptidases / Q000378:metabolism*; D007202:Indicators and Reagents; D007700:Kinetics; D010434:Pepsin A / Q000378:metabolism; D010455:Peptides / Q000138:chemical synthesis*; D012995:Solubility; D012997:Solvents; D013820:Thermolysin / Q000378:metabolism; D014867:Water
D000596:Amino Acids; D007202:Indicators and Reagents; D010455:Peptides; D012997:Solvents; D014867:Water; D010450:Endopeptidases; D010434:Pepsin A; D013820:Thermolysin