Coculture of newborn rat skin epidermal cells with Swiss 3T3 cells: effect of the phases of 3T3 cells on attachment, growth and keratin synthesis of epidermal cells.
Swiss albino mouse 3T3 cells in various states were inoculated onto one side of Millipore filters. The other side of the filter was then coated with type I collagen and inoculated with newborn rat skin epidermal cells. On coculture of these cells, the attachment, growth and keratin synthesis of epidermal cells were found to depend on the state of the 3T3 cells: 3T3 cells in the stationary phase of growth were the most effective, followed by those in the logarithmic growth phase, those in the lag phase and plasmolyzed fibroblasts being only slightly effective. The effects of 3T3 cells in different states correlated well with their abilities to synthesize type IV collagen, but not type I collagen: with an increase in type IV collagen synthesis by the 3T3 cells, attachment of epidermal cells to the cell support, and their growth and synthesis of keratins increased. This culture system is concluded to mimic conditions in skin in vivo, and therefore to be suitable for studies on the effects of fibroblasts on the growth of epidermal cells.
Ohkura
K
K
Faculty of Pharmaceutical Sciences, University of Tokushima, Japan.
Terada
H
H
D016475:3T3 Cells; D000818:Animals; D000831:Animals, Newborn; D002448:Cell Adhesion; D002450:Cell Communication; D002455:Cell Division; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis; D000078404:Epidermal Cells; D004817:Epidermis / Q000254:growth & development / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism*; D007633:Keratins / Q000096:biosynthesis*; D051379:Mice; D051381:Rats; D011919:Rats, Inbred Strains; D012867:Skin / Q000166:cytology* / Q000254:growth & development / Q000378:metabolism
D007633:Keratins; D003094:Collagen