Relative performance of Organon kit in comparison to Du Pont for confirmatory serological testing of HIV infection by western blot test in sera from blood donors.
A total of 32 specimens with different categories of reactivity by Du Pont Western Blot kit comprising of specimens showing full spectrum of HIV-I antigen specific bands, 19 specimens showing total absence of bands and four specimens showing non-specific bands (without any interpretative importance) were subjected to Western Blot testing by Organon test. Of the nine specimens showing full spectrum of bands by Du Pont the correlation with Organon kit was 100 per cent based on WHO criteria. Four specimens with non-specific indeterminate band pattern by Du Pont failed to show any band in Organon kit, indicating that latter to be more specific.
National Institute of Communicable Diseases, Sham Nath Marg, Delhi.
R K
RK
Aggarwal
R
R
Chatterjee
D
D
Chattopadhya
S
S
Kumari
D015492:AIDS Serodiagnosis / Q000379:methods*; D001782:Blood Donors; D015153:Blotting, Western / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay; D005069:Evaluation Studies as Topic; D015658:HIV Infections / Q000097:blood* / Q000453:epidemiology / Q000517:prevention & control; D015497:HIV-1; D006801:Humans; D008403:Mass Screening / Q000379:methods; D011933:Reagent Kits, Diagnostic / Q000592:standards*; D012680:Sensitivity and Specificity
D011933:Reagent Kits, Diagnostic