Stabilization of the adenylate energy charge in erythrocytes of rats and humans at high altitude hypoxia.
1. Stabilization of adenylate energy charge and control of adenylate pool were analysed in the erythrocytes of the rat and the human exposed to highly hypoxic conditions. 2. Red cell energy charge was decreased in the rats exposed to a simulated altitude of 5000-8000 m, and then recovered to the normal value with the depletion of adenylate pool. 3. The energy charge and the adenylate pool size of the human erythrocytes did not show any change under highly hypoxic conditions. 4. Anaerobic incubation of rat erythrocytes caused a marked decrease in the energy charge, and its recovery was accompanied by the depletion of total adenylates. 5. The energy charge and total adenylates of human red cells did not change under the anaerobic incubation of erythrocytes. 6. These results suggest that the energy charge of rat erythrocytes can be controlled by depletion of the adenylate pool, but the adenylate degradation is not responsible for the stabilization of the energy charge in human erythrocytes.
Department of Genetics, Institute for Developmental Research, Aichi, Japan.
M
M
Yoshino
C
C
Yamamoto
K
K
Murakami
Y
Y
Katsumata
S
S
Mori
D000244:Adenosine Diphosphate / Q000097:blood; D000249:Adenosine Monophosphate / Q000097:blood* / Q000737:chemistry; D000255:Adenosine Triphosphate / Q000097:blood; D000328:Adult; D000532:Altitude Sickness / Q000097:blood*; D000818:Animals; D004734:Energy Metabolism; D004912:Erythrocytes / Q000378:metabolism*; D006801:Humans; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
D000249:Adenosine Monophosphate; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate