Molecular analysis of a Bjerkandera adusta lignin peroxidase gene.
A cDNA clone, lambda LPO-1, encoding a major lignin peroxidase from the basidiomycete Bjerkandera adusta was isolated and characterized. The nucleotide sequence of lambda LPO-1 predicts a mature protein consisting of 349 amino acids with a molecular weight of 37,225 preceded by a signal peptide of 23 amino acid residues. We have also cloned and sequenced the gene encoding lignin peroxidase from B. adusta. Comparison of these sequences reveals a lignin peroxidase gene structure consisting of 1,116 bp of protein-encoding DNA that is interrupted by four intervening sequences. The putative eukaryotic regulatory sequence, a TATA box, is present at position -75 relative to the translational initiation codon. Amino acid sequence homology between the coding regions of lambda LPO-1 and of the lignin peroxidase cDNA clone lambda ML-1 from Phanerochaete chrysosporium is 61%.
Kimura
Y
Y
Department of Bioresource Science, Faculty of Agriculture, Kagawa University, Japan.
Asada
Y
Y
Oka
T
T
Kuwahara
M
M
D000595:Amino Acid Sequence; D001483:Base Sequence; D001487:Basidiomycota / Q000201:enzymology / Q000235:genetics*; D004271:DNA, Fungal / Q000737:chemistry; D005800:Genes, Fungal; D015698:Genomic Library; D008969:Molecular Sequence Data; D010544:Peroxidases / Q000235:genetics*; D015183:Restriction Mapping; D016385:TATA Box
D004271:DNA, Fungal; D010544:Peroxidases; C042858:lignin peroxidase