Detection and quantification of anti-Ki antibodies by enzyme-linked immunosorbent assay using recombinant Ki antigen.
OBJECTIVE
To establish an enzyme-linked immunosorbent assay (ELISA) for detecting anti-Ki antibody, using a bovine recombinant Ki antigen, and studying its specificity.


METHODS
Sera from 220 patients with various connective tissue diseases were screened, and a prospective study of fluctuations in anti-Ki antibody and clinical course of a woman with systemic lupus erythematosus (SLE) was analyzed, by ELISA:


RESULTS
Anti-Ki antibodies were present in 18.9% of patients with SLE. The titer of anti-Ki antibody in the woman with SLE rose before the onset of pericarditis and pleuritis in this longitudinal study.


CONCLUSIONS
ELISA using a recombinant Ki antigen is useful for the diagnosis of SLE, and it might be useful in estimating disease activity in patients with SLE.
Department of Internal Medicine, Juntendo University, School of Medicine, Tokyo, Japan.
K
K
Yamanaka
Y
Y
Takasaki
Y
Y
Nishida
K
K
Shimada
M
M
Shibata
H
H
Hashimoto
D000818:Animals; D001323:Autoantibodies / Q000032:analysis*; D002417:Cattle; D002846:Chromatography, Affinity; D003240:Connective Tissue Diseases / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000276:immunology; D009687:Nuclear Proteins / Q000737:chemistry / Q000276:immunology*; D010455:Peptides / Q000032:analysis; D018809:Proliferating Cell Nuclear Antigen; D011994:Recombinant Proteins
D001323:Autoantibodies; D009687:Nuclear Proteins; D010455:Peptides; D018809:Proliferating Cell Nuclear Antigen; D011994:Recombinant Proteins