Chromatography of hen egg-white proteins on anion-exchange cellulose at high flow rates using a radial flow column.
The influence of column configuration on the separation of hen egg-white proteins using Whatman DE52 and QA52 anion-exchange cellulose has been investigated. Using a 100 ml volume axial flow column (6.6 cm x 4.4 cm i.d.) we achieved flow rates of up to 25 ml/min i.e. 15 bed volumes/h after which higher flow was restricted due to pressure constraints within the system. Under radial flow conditions using a 100 ml column flow rates of up to 150 ml/min i.e. 90 bed volumes/h were achieved using DE52 and QA52. While chromatographic resolution was superior under axial flow at the lower flow rates excellent resolution was maintained at up to 150 ml/min using the radial flow column. This is a consequence of the fast kinetics of adsorption/desorption exhibited by DE52 and QA52. The data indicate that it is the column configuration and not the cellulose matrix which influences flow performance.
Whatman Specialty Products Division, Media Technology Operations (U.S.), Fairfield, N.J. 07004.
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Koscielny
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Levison
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Toome
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Butts
D000818:Animals; D002482:Cellulose; D002645:Chickens; D002852:Chromatography, Ion Exchange / Q000379:methods*; D004527:Egg Proteins / Q000302:isolation & purification*; D007700:Kinetics
D004527:Egg Proteins; D002482:Cellulose