Nucleolar organizer regions in squamous cell carcinoma of the head and neck.
Nucleolar organizer regions are collections of nucleolar proteins associated with ribosomal genes that can be visualized in histologic sections using a silver colloid stain, thus the term silver-staining nucleolar organizer region (AgNOR). In some tissues, the number of AgNORs per nucleus correlates with cellular proliferation and, independently, with malignant change. AgNORs were studied in 66 paraffin-embedded head and neck squamous cell carcinomas and in 12 samples of normal tonsillar squamous epithelium. Carcinomas had a significantly higher mean AgNOR count than the benign epithelium (P less than .0001). Among carcinomas, mean AgNOR count increased with stage of the disease (P less than .001), but there was no significant correlation with histologic grade or DNA ploidy as determined by flow cytometry. These data suggest that AgNOR count should be evaluated as a possible aid in differentiating benign from malignant squamous epithelial proliferations in the head and neck, and also possibly as a prognostic marker in these carcinomas.
Department of Otolaryngology and Bronchoesophagology, Rush-Presbyterian-St. Luke's Medical Center, Chicago, IL 60612.
S M
SM
Hirsch
J
J
DuCanto
D D
DD
Caldarelli
J C
JC
Hutchinson
J S
JS
Coon
D000782:Aneuploidy; D002294:Carcinoma, Squamous Cell / Q000473:pathology / Q000648:ultrastructure*; D004273:DNA, Neoplasm / Q000032:analysis* / Q000235:genetics; D004848:Epithelium / Q000648:ultrastructure; D005434:Flow Cytometry; D006258:Head and Neck Neoplasms / Q000473:pathology / Q000648:ultrastructure*; D006801:Humans; D007012:Hypopharyngeal Neoplasms / Q000473:pathology / Q000648:ultrastructure; D007822:Laryngeal Neoplasms / Q000473:pathology / Q000648:ultrastructure; D009367:Neoplasm Staging; D009697:Nucleolus Organizer Region / Q000648:ultrastructure*; D009959:Oropharyngeal Neoplasms / Q000473:pathology / Q000648:ultrastructure; D011003:Ploidies; D013194:Staining and Labeling; D014062:Tongue Neoplasms / Q000473:pathology / Q000648:ultrastructure
D004273:DNA, Neoplasm