Stimulation of neutrophil adhesion by antibodies recognizing CD15 (Le(X)) and CD15-expressing carcinoembryonic antigen-related glycoprotein NCA-160.
The carbohydrate antigen, CD15 (Le(X)), and its sialylated derivative have recently been shown to be involved in the binding of neutrophils to the endothelial lectins, E-selectin and P-selectin. Neutrophil NCA-160, a carcinoembryonic antigen (CEA)-related glycoprotein, is the major carrier of CD15, which is also expressed on the common beta 2 chain of leucocyte integrins. Rabbit IgG antibodies directed against CEA, which cross-react with neutrophil NCAs, increase the adhesion of neutrophils to plastic. This effect is also observed with F(ab')2 and Fab antibody fragments and a monoclonal antibody (mAb) recognizing the same antigen. Anti-CD15 mAbs inhibit adhesion at higher concentrations, but augment adhesion at lower concentrations. Anti-CEA and anti-CD15 antibodies cause the homotypic adhesion of neutrophils demonstrable by light microscopy and flow cytometry. Anti-(integrin beta 2 chain) mAbs inhibit both adhesion to plastic and homotypic adhesion. These results suggest that binding of ligand to NCA-160 is able to trigger neutrophil adhesion events which have been shown to be integrin mediated. Anti-CD15 mAbs do not, however, induce a respiratory burst from neutrophils.
Department of Pathology, University of Dundee, Ninewells Hospital Medical School, U.K.
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Stocks
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Kerr
D000906:Antibodies; D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000276:immunology / Q000502:physiology*; D000951:Antigens, Neoplasm; D002272:Carcinoembryonic Antigen / Q000276:immunology; D002448:Cell Adhesion / Q000502:physiology*; D015815:Cell Adhesion Molecules; D006801:Humans; D007140:Immunoglobulin Fab Fragments; D016023:Integrins / Q000276:immunology / Q000502:physiology; D016256:Lewis X Antigen; D008163:Luminescent Measurements; D008562:Membrane Glycoproteins / Q000276:immunology / Q000502:physiology*; D009504:Neutrophils / Q000502:physiology*
D000906:Antibodies; D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000951:Antigens, Neoplasm; D002272:Carcinoembryonic Antigen; D015815:Cell Adhesion Molecules; D007140:Immunoglobulin Fab Fragments; D016023:Integrins; D016256:Lewis X Antigen; D008562:Membrane Glycoproteins