Interferon-gamma regulates expression of a novel keratin class I gene.
Interferon (IFN)-gamma has been implicated in the pathogenesis of several autoimmune disorders and inflammatory skin diseases. To identify novel mediators involved in the IFN-gamma response we have used differential hybridization of a cDNA library prepared from IFN-gamma-treated HeLa cells to isolate a gene that is induced following treatment with IFN-gamma. We report here the molecular cloning and characterization of a cDNA detecting a 1.6-kb mRNA that accumulated in response to IFN-gamma but not in response to IFN-alpha or IFN-beta. The gene is regulated by IFN-gamma in human cell lines of epithelial origin. The mRNA encodes a predicted protein of 432 amino acids and the primary structure of the protein demonstrates that it is a novel member of developmentally regulated keratin class I genes.
Department of Dermatology, University of Cologne, FRG.
T
T
Flohr
U
U
Buwitt
B
B
Bonnekoh
T
T
Decker
E C
EC
Böttger
D000595:Amino Acid Sequence; D003513:Cycloheximide / Q000494:pharmacology; D004848:Epithelium / Q000502:physiology; D015870:Gene Expression / Q000187:drug effects; D005796:Genes; D006367:HeLa Cells; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology*; D007633:Keratins / Q000235:genetics*; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics; D016415:Sequence Alignment
D012333:RNA, Messenger; D007633:Keratins; D007371:Interferon-gamma; D003513:Cycloheximide