Widespread co-localization of mRNAs encoding the guanylate cyclase-coupled natriuretic peptide receptors in rat tissues.
Natriuretic peptides modulate vasorelaxation, diuresis, and natriuresis through the stimulation of cGMP production by the guanylate cyclase-coupled natriuretic peptide receptors, GC-A and GC-B. We used reverse transcription-polymerase chain reaction to determine the distribution of mRNA encoding both receptors in rat tissues. GC-A and GC-B transcripts were detected in all peripheral and neural tissues examined. Since the atrial natriuretic peptide gene is expressed in all these tissues, our widespread detection of GC-A and GC-B mRNAs now suggests that natriuretic peptides may act as endocrine and paracrine hormones as well as neurotransmitters via both GC-A and GC-B receptors.
Department of Physiology, University of Toronto, Ontario, Canada.
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D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000378:metabolism*; D006162:Guanylate Cyclase / Q000235:genetics*; D008297:Male; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D009928:Organ Specificity; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D017461:Receptors, Atrial Natriuretic Factor / Q000235:genetics*; D015183:Restriction Mapping; D014158:Transcription, Genetic
D009838:Oligodeoxyribonucleotides; D012333:RNA, Messenger; D006162:Guanylate Cyclase; D017461:Receptors, Atrial Natriuretic Factor