More on effects of storage time and temperature on urinary enzymes: a 1-year study.
Results of our conclusive study on urinary enzyme stability during sample storage are reported. We measured alanine aminopeptidase (AAP) and N-acetyl-beta-D-glucosaminidase (NAG) in morning urines from 9 healthy normal subjects immediately after collection and throughout a 1-year storage at -70 and -20 degrees C. AAP proved to be quite stable at -70 degrees C (99.2% of the basal value at the end of the year). NAG is partially preserved (84.1% of the basal value) at -70 degrees C, but significantly decreased (50.4%) at -20 degrees C.
Postgraduate Medical School of Clinical Pathology, University of Pisa, Italy.
E
E
Matteucci
L
L
Pellegrini
C
C
Uncini-Manganelli
M
M
Cecere
M
M
Saviozzi
O
O
Giampietro
D000118:Acetylglucosaminidase / Q000652:urine*; D000328:Adult; D000626:Aminopeptidases / Q000652:urine*; D018826:CD13 Antigens; D004795:Enzyme Stability; D005260:Female; D005615:Freezing; D006801:Humans; D008297:Male; D012016:Reference Values; D013997:Time Factors
D000118:Acetylglucosaminidase; D000626:Aminopeptidases; D018826:CD13 Antigens