Cocaine induced synchronous oscillations in central noradrenergic neurons in vitro.
Through inhibition of reuptake, cocaine increases monoaminergic tone in the central nervous system. The activities of the neurons within the locus coeruleus play a pivotal role in central noradrenergic transmission and regulate overall levels of arousal and attention. We have found that cocaine in low concentrations (0.3-1.0 microM) induced slow oscillations (0.8 Hz) in membrane potential (2-6 mV). These oscillations were synchronized in neurons throughout the nucleus and were blocked by alpha 2-adrenergic receptor antagonists. The synchrony of these events was thought to arise from within the nucleus, through a combination of spontaneous activity (intrinsic properties) and noradrenergic mediated inhibitory postsynaptic potentials augmented by cocaine. The synchronous firing of noradrenergic neurons may facilitate transmitter release in the widespread projection areas and thus be important for the action of cocaine to increase levels of arousal.
G C
GC
Harris
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Hausken
J T
JT
Williams
D000317:Adrenergic alpha-Antagonists / Q000494:pharmacology*; D000818:Animals; D003042:Cocaine / Q000494:pharmacology*; D004146:Dioxanes / Q000494:pharmacology*; D005071:Evoked Potentials / Q000187:drug effects; D019329:Idazoxan; D066298:In Vitro Techniques; D007700:Kinetics; D007736:Kynurenic Acid / Q000494:pharmacology; D008125:Locus Coeruleus / Q000502:physiology*; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D009991:Oscillometry; D010852:Picrotoxin / Q000494:pharmacology; D011224:Prazosin / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D013331:Strychnine / Q000494:pharmacology; D013569:Synapses / Q000187:drug effects / Q000502:physiology
D000317:Adrenergic alpha-Antagonists; D003042:Cocaine; D004146:Dioxanes; D019329:Idazoxan; D007736:Kynurenic Acid; D010852:Picrotoxin; D011224:Prazosin; D013331:Strychnine