Simultaneous high-performance liquid chromatographic analysis of buspirone and its metabolite 1-(2-pyrimidinyl)-piperazine in plasma using electrochemical detection.
A selective and sensitive high-performance liquid chromatographic method with coulometric detection is described for the quantitation of buspirone and its active metabolite, 1-(2-pyrimidinyl)piperazine, in plasma samples of mice treated orally with buspirone (10 mg/kg body weight). The analytes are extracted with a carboxylic acid solid-phase extraction column before chromatography. A dual-electrode electrochemical detector is used. The limit of detection is 50 pg for buspirone and 35 pg for 1-(2-pyrimidinyl)piperazine.
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Istituto Superiore di Sanità, Rome, Italy.
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D000284:Administration, Oral; D000818:Animals; D014151:Anti-Anxiety Agents / Q000097:blood*; D002065:Buspirone / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004563:Electrochemistry; D008297:Male; D051379:Mice
D014151:Anti-Anxiety Agents; C037921:1-(2-pyrimidinyl)piperazine; D002065:Buspirone