Production of monoclonal antibodies by hybridoma cells in a flat sheet membrane bioreactor.
The purpose of this study was to investigate hybridoma growth and monoclonal antibody formation in a flat sheet membrane bioreactor. The effects of several different molecular weight cutoff membranes on growth and antibody formation were investigated. Nutrient and toxic product diffusion through the membranes were quantified, and the kinetic and physical constants of the system were determined.
Hagedorn
J
J
Department of Chemical Engineering, Washington University, St. Louis, Missouri 63130.
Kargi
F
F
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D001709:Biotechnology / Q000295:instrumentation / Q000379:methods*; D002460:Cell Line; D005947:Glucose / Q000378:metabolism; D006825:Hybridomas; D007700:Kinetics; D007773:Lactates / Q000378:metabolism; D019344:Lactic Acid; D008566:Membranes; D051379:Mice; D008970:Molecular Weight
D000911:Antibodies, Monoclonal; D007773:Lactates; D019344:Lactic Acid; D005947:Glucose