Anchorage-dependent mammalian cell culture using polyurethane foam as a new substratum for cell attachment.
Anchorage-dependent mammalian cells were cultivated at high cell density in a novel culture system using polyurethane foam (PUF) as a substratum for cell attachment. PUF has a macroporous structure giving a high surface area to volume ratio. Monkey kidney cells (Vero) and Chinese hamster ovary cells (CHO-K1) attached to the internal surface of PUF and grew to a high cell density (1.04 X 10(8) cells/cm3 PUF and 3.5 X 10(7) cells/cm3 PUF, respectively) in PUF stationary cultures. In addition, we have designed a PUF-particle packed-bed culture system for high density mass cell culture. A maximum cell density of 2.4 X 10(7) cells/cm3 culture vessel volume was obtained in a packed-bed culture of Vero cells.
Department of Chemical Engineering, Faculty of Engineering, Kyushu University, Fukuoka, Japan.
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Matsushita
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Ketayama
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Kamihata
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Funatsu
D000818:Animals; D001709:Biotechnology; D002448:Cell Adhesion; D002452:Cell Count; D002478:Cells, Cultured; D006224:Cricetinae; D003412:Cricetulus; D000882:Haplorhini; D011140:Polyurethanes; D014709:Vero Cells / Q000502:physiology
D011140:Polyurethanes