Creutzfeldt-Jakob disease cosegregates with the codon 178Asn PRNP mutation in families of European origin.
We recently discovered an amino acid-altering heterozygous mutation in codon 178 of the PRNP amyloid precursor gene in patients with familial Creutzfeldt-Jakob disease. This mutation is now shown to be associated with the occurrence of disease in 7 unrelated families of Western European origin, among which a total of 65 members are known to have died from Creutzfeldt-Jakob disease. The mutation was detected in each of 17 tested patients, including at least 1 affected member of each family, and in 16 of 36 of their first-degree relatives, but not in affected families with other mutations, patients with the nonfamilial form of the disease, or 83 healthy control individuals. Linkage analysis in two informative families yielded a lod score of 5.30, which, because no recombinants were found, strongly suggests that codon 178Asn is the actual disease mutation.
Laboratory of Central Nervous System Studies, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892.
L G
LG
Goldfarb
P
P
Brown
M
M
Haltia
F
F
Cathala
W R
WR
McCombie
J
J
Kovanen
L
L
Cervenáková
L
L
Goldin
A
A
Nieto
M S
MS
Godec
D000328:Adult; D001483:Base Sequence; D003062:Codon; D007562:Creutzfeldt-Jakob Syndrome / Q000208:ethnology / Q000235:genetics*; D004252:DNA Mutational Analysis; D005060:Europe / Q000208:ethnology; D005260:Female; D005796:Genes; D005799:Genes, Dominant; D020022:Genetic Predisposition to Disease; D006801:Humans; D007729:Kuru / Q000235:genetics; D008126:Lod Score; D008297:Male; D008969:Molecular Sequence Data; D009154:Mutation; D010375:Pedigree; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D018621:PrPC Proteins; D011328:Prions / Q000235:genetics* / Q000648:ultrastructure; D011487:Protein Conformation; D011498:Protein Precursors / Q000235:genetics* / Q000648:ultrastructure
D003062:Codon; D018621:PrPC Proteins; D011328:Prions; D011498:Protein Precursors