Growth enhancement in transgenic Atlantic salmon by the use of an "all fish" chimeric growth hormone gene construct.
We have developed an "all fish" growth hormone (GH) chimeric gene construct by using an antifreeze protein gene (AFP) promoter from ocean pout linked to a chinook salmon GH cDNA clone. After microinjection into fertilized, nonactivated Atlantic salmon eggs via the micropyle, transgenic Atlantic salmon were generated. The presence of the transgene was detected by polymerase chain reaction (PCR) using specific oligonucleotide primers. A number of these transgenic fish showed dramatic increases in their growth rate. At one year old, the average increase of the transgenic fish was 2 to 6 fold and the largest transgenic fish was 13 times that of the average non-transgenic control.
Research Institute, Hospital for Sick Children, Toronto, Canada.
S J
SJ
Du
Z Y
ZY
Gong
G L
GL
Fletcher
M A
MA
Shears
M J
MJ
King
D R
DR
Idler
C L
CL
Hew
D000818:Animals; D030801:Animals, Genetically Modified / Q000254:growth & development*; D021301:Antifreeze Proteins; D001483:Base Sequence; D001773:Blood Cells / Q000502:physiology; D001835:Body Weight; D002678:Chimera; D003001:Cloning, Molecular; D004247:DNA / Q000008:administration & dosage / Q000097:blood / Q000235:genetics; D005399:Fishes / Q000235:genetics*; D005615:Freezing; D006023:Glycoproteins / Q000235:genetics*; D013006:Growth Hormone / Q000235:genetics* / Q000502:physiology; D008845:Microinjections; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D010063:Ovum / Q000502:physiology; D016133:Polymerase Chain Reaction / Q000379:methods; D011401:Promoter Regions, Genetic; D012474:Salmon / Q000235:genetics / Q000254:growth & development*; D014284:Triiodothyronine / Q000097:blood
D021301:Antifreeze Proteins; D006023:Glycoproteins; D015345:Oligonucleotide Probes; D014284:Triiodothyronine; D013006:Growth Hormone; D004247:DNA