Sperabillins, new antibacterial antibiotics with potent in vivo activity. Taxonomy, fermentation, isolation and biological activity.
A Gram-negative bacterium was found to produce new antibacterial antibiotics, sperabillins A, B, C and D, and the producing bacterium was characterized and identified as Pseudomonas fluorescens YK-437. Sperabillins were isolated by column chromatographies using cation-exchange resins, activated carbon and cation-exchange Sephadex, and preparative reverse-phase HPLC. Sperabillins showed antibacterial activity against Gram-negative and Gram-positive bacteria including antibiotic-resistant strains of Pseudomonas aeruginosa and Staphylococcus aureus. Sperabillin A inhibited DNA, RNA, protein, and cell wall biosynthesis in Escherichia coli. Sperabillins showed good protective effects in experimentally infected mice.
Microbiology Research Laboratories, Takeda Chemical Industries, Ltd., Osaka, Japan.
N
N
Katayama
Y
Y
Nozaki
S
S
Tsubotani
M
M
Kondo
S
S
Harada
H
H
Ono
D000578:Amidines / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D000818:Animals; D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects*; D001424:Bacterial Infections / Q000188:drug therapy; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000187:drug effects; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D003470:Culture Media; D004269:DNA, Bacterial / Q000096:biosynthesis / Q000187:drug effects; D005285:Fermentation; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D010457:Peptidoglycan / Q000096:biosynthesis / Q000187:drug effects; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D011551:Pseudomonas fluorescens / Q000145:classification / Q000378:metabolism*; D012329:RNA, Bacterial / Q000096:biosynthesis / Q000187:drug effects; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism
D000578:Amidines; D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D003470:Culture Media; D004269:DNA, Bacterial; D010457:Peptidoglycan; D012329:RNA, Bacterial; C073531:sperabillin C; C073530:sperabillin B; C073529:sperabillin A; C073532:sperabillin D