Decrease by psychotropic drugs and local anaesthetics of membrane fluidity measured by fluorescence anisotropy in Escherichia coli.
The effects of psychotropic drugs and local anaesthetics on the fluidity of Escherichia coli cell membranes were examined. Chlorpromazine was shown to increase 1,6-diphenyl-1,3,5-hexatriene fluorescence anisotropy, indicating that it decreased the membrane fluidity. This increase was significant at a temperature of more than 24 degrees C. Dibucaine, lignocaine, imipramine, tetracaine and procaine also increased the fluorescence anisotropy.
Faculty of Pharmaceutical Sciences, University of Tokyo, Japan.
K
K
Tanji
Y
Y
Ohta
S
S
Kawato
T
T
Mizushima
S
S
Natori
K
K
Sekimizu
D000779:Anesthetics, Local / Q000494:pharmacology*; D002462:Cell Membrane / Q000187:drug effects*; D002746:Chlorpromazine / Q000494:pharmacology*; D004926:Escherichia coli; D005454:Fluorescence Polarization; D008560:Membrane Fluidity / Q000187:drug effects
D000779:Anesthetics, Local; D002746:Chlorpromazine