[Detection of human papillomavirus (HPV) DNA in carcinoma of uterine cervix and genital tract diseases in Jiangsu province, China].
Pst-1 cleaved DNA restriction fragment length polymorphism (RFLP) of biopsy samples from 41 patients with squamous cell carcinoma of uterine cervix collected in Jiangsu province, China were examined for HPVs by Southern blot hybridization using HPV 16 DNA as a probe. 20 of the 41 samples were positive for HPVs when hybridized under non-stringent condition. HPV was not detectable in samples collected in the same time period from patients with cervical adenocarcinoma (N = 2), vaginal carcinoma (N = 3), vulval carcinoma (N = 1) and benign cervicitis (N = 8). Of the 20 positive samples, 7 (17.1%) had CHPV X1, a new type of NPV previously discovered is China, 6 (14.6%) had HPV 16, 4 (9.9%) had HPV 31, and in 3 (7.2%) the HPV type is as yet undetermined. Our data indicate that HPV 16 and CHPV X1 may be more closely related to cancer of the uterine cervix in Jiangsu province.
Jiangsu Institute for Cancer Research, Nanjing.
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D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002294:Carcinoma, Squamous Cell / Q000382:microbiology*; D015344:DNA Probes, HPV; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D006801:Humans; D008875:Middle Aged; D027383:Papillomaviridae / Q000145:classification / Q000302:isolation & purification*; D012150:Polymorphism, Restriction Fragment Length; D002583:Uterine Cervical Neoplasms / Q000382:microbiology*
D015344:DNA Probes, HPV; D004279:DNA, Viral