Hypotensive effect associated with a phospholipase C-delta 1 gene mutation in the spontaneously hypertensive rat.
To identify the genes responsible for blood pressure in the spontaneously hypertensive rat strain, we performed a cosegregation analysis between the genotype and blood pressure in a set of male F2 rats obtained by crossmating SHR with Wistar-Kyoto rats, a parental normotensive strain. Our investigation revealed that the phospholipase C-delta 1 polymorphism, which resulted in missense mutation, cosegregates with the lower blood pressure in SHR, and that PLC-delta 1 gene is located on chromosome 8. On the other hand, we found the lack of cosegregation between blood pressure and the nerve growth factor receptor gene, which is linked to a hypertensinogenic gene locus (denoted as BP/SP-1) on chromosome 10. We propose that PLC-delta 1 gene itself of closely linked gene on chromosome 8 is a new candidate with the hypotensive effect, and that BP-SP1 locus does not directly contribute to blood pressure elevation in original SHR.
Department of Geriatric Medicine, Osaka University Medical School, Japan.
T
T
Katsuya
J
J
Higaki
T
T
Miki
K
K
Kohara
H
H
Yagisawa
H
H
Tanase
H
H
Mikami
T
T
Serikawa
H
H
Nojima
T
T
Ogihara
D000818:Animals; D001483:Base Sequence; D001794:Blood Pressure; D002874:Chromosome Mapping; D006973:Hypertension / Q000235:genetics*; D008297:Male; D008969:Molecular Sequence Data; D009154:Mutation; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D051381:Rats; D011918:Rats, Inbred SHR / Q000235:genetics*; D011921:Rats, Inbred WKY / Q000235:genetics; D017475:Receptors, Nerve Growth Factor / Q000235:genetics; D010738:Type C Phospholipases / Q000235:genetics*
D009838:Oligodeoxyribonucleotides; D017475:Receptors, Nerve Growth Factor; D010738:Type C Phospholipases