The binding site on ICAM-1 for Plasmodium falciparum-infected erythrocytes overlaps, but is distinct from, the LFA-1-binding site.
The intercellular adhesion molecule-1 (ICAM-1, CD54) is one of three putative endothelial receptors that mediate in vitro cytoadherence of P. falciparum-infected erythrocytes. Since cytoadherence to postcapillary venular endothelium is thought to be a major factor in the virulence of P. falciparum malaria, we have examined the interaction between ICAM-1 and the P. falciparum-infected cell, and have compared it with the interaction to the physiological counter receptor, the leukocyte integrin LFA-1. Our results demonstrate that the malaria-binding site resides in the first two domains of the ICAM-1 molecule and overlaps, but is distinct from, the LFA-1 site.
Berendt
A R
AR
Molecular Parasitology Group, Institute of Molecular Medicine, John Radcliffe Hospital, Oxford, England.
McDowall
A
A
Craig
A G
AG
Bates
P A
PA
Sternberg
M J
MJ
Marsh
K
K
Newbold
C I
CI
Hogg
N
N
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D001483:Base Sequence; D001665:Binding Sites; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D000939:Epitopes / Q000032:analysis; D004912:Erythrocytes / Q000469:parasitology / Q000502:physiology*; D006801:Humans; D018799:Intercellular Adhesion Molecule-1; D016169:Lymphocyte Function-Associated Antigen-1 / Q000502:physiology*; D016778:Malaria, Falciparum / Q000097:blood*; D051379:Mice; D008958:Models, Molecular; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009838:Oligodeoxyribonucleotides; D010963:Plasmodium falciparum / Q000502:physiology*; D011487:Protein Conformation; D011991:Receptors, Virus / Q000502:physiology*; D012689:Sequence Homology, Nucleic Acid
D000911:Antibodies, Monoclonal; D015815:Cell Adhesion Molecules; D000939:Epitopes; D016169:Lymphocyte Function-Associated Antigen-1; D009838:Oligodeoxyribonucleotides; D011991:Receptors, Virus; D018799:Intercellular Adhesion Molecule-1