Cloning and partial sequencing of the porcine growth hormone (pGH) gene from pituitary gland.
High molecular weight DNA was isolated from porcine pituitary gland, and a genomic library was constructed using lambda EMBL3 as cloning vector. Five positive clones were identified by in-situ hybridization with the full-length pGH cDNA as the probe. Dot-blot hybridization, restriction analysis, and Southern hybridization showed that one of the positive clones contained the entire pGH gene. A subclone that contained the upstream sequence from the SmaI site of pGH gene was identified, and part of it was sequenced. We also compared the sequence of our pGH gene with the corresponding sequence of the published pGH gene.
Laboratory of Animal Biochemistry, Beijing Agricultural University, China.
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Q
Yang
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B
Zhu
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Zhou
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Qi
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004247:DNA; D015723:Gene Library; D005822:Genetic Vectors; D013006:Growth Hormone / Q000235:genetics*; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D010902:Pituitary Gland / Q000378:metabolism*; D013552:Swine
D013006:Growth Hormone; D004247:DNA