Isolation of a cDNA encoding a human serum marker for acute pancreatitis. Identification of pancreas-specific protein as pancreatic procarboxypeptidase B.
A human pancreas-specific protein (PASP), previously characterized as a serum marker for acute pancreatitis and pancreatic graft rejection, has been identified as pancreatic procarboxypeptidase B (PCPB). cDNAs encoding PASP/PCPB were isolated from a human pancreas cDNA library using a combination of nucleic acid hybridization screening and immunoscreening with antisera raised against native PASP. The deduced amino acid sequence of PASP/PCPB cDNA predicts the translation of a 416-amino acid preproenzyme with a 15-amino acid signal/leader peptide and a 95-amino acid activation peptide. The proenzyme portion of this protein has 76% identity with rat PCPB and 84% identity with bovine carboxypeptidase B. DNA and RNA blot analyses indicate that human PCPB mRNA (1,400 nucleotides) is transcribed from a single locus in the human genome in a tissue-specific fashion. N-terminal sequencing of native PASP and the specific immunoreactivity of bacterially expressed PASP/PCPB with native PASP antibodies confirm the identification of PASP as human pancreatic PCPB.
Yamamoto
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Molecular Biology Department, Diagnostic Products Corporation, Los Angeles, California 90045.
Pousette
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Chow
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Wilson
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el Shami
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French
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D000208:Acute Disease; D000595:Amino Acid Sequence; D001483:Base Sequence; D015415:Biomarkers / Q000097:blood; D015152:Blotting, Northern; D015139:Blotting, Southern; D015153:Blotting, Western; D043424:Carboxypeptidase B; D002268:Carboxypeptidases / Q000097:blood / Q000235:genetics*; D004247:DNA / Q000235:genetics / Q000302:isolation & purification*; D004792:Enzyme Precursors / Q000097:blood / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010195:Pancreatitis / Q000097:blood* / Q000175:diagnosis; D016133:Polymerase Chain Reaction; D011506:Proteins / Q000235:genetics* / Q000378:metabolism; D012313:RNA / Q000235:genetics; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid
D015415:Biomarkers; C058173:CPB1 protein, human; D004792:Enzyme Precursors; D011506:Proteins; D012313:RNA; D004247:DNA; D002268:Carboxypeptidases; D043424:Carboxypeptidase B