Endopeptidase 24.15 modulates bradykinin-induced contraction in guinea-pig trachea.
Guinea-pig tracheal strips were contracted with cumulative concentrations of bradykinin or substance P in the presence of thiorphan, an inhibitor of endopeptidase 24.11 and of angiotensin-converting enzyme, or in the presence of N-[1-(R,S)-carboxy-3-phenylpropyl]Ala-Ala-Phe-para-aminobenzoate (cFP-AAF-pAB), a selective inhibitor of endopeptidase 24.15. The concentration-effect curve of bradykinin was shifted to the left in the presence of each inhibitor whereas the curve of substance P was sensitive to thiorphan but not to cFP-AAF-pAB. These results show that endopeptidase 24.15 may modulate the contractile effect of bradykinin but not that of substance P in the guinea-pig trachea.
Laboratoire de Neuroimmunopharmacologie, Université Louis Pasteur--Strasbourg I, Illkirch, France.
A
A
Da Silva
J
J
Dhuy
F
F
Waeldelé
C
C
Bertrand
Y
Y
Landry
D000595:Amino Acid Sequence; D000818:Animals; D001920:Bradykinin / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D006168:Guinea Pigs; D008297:Male; D008666:Metalloendopeptidases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D008969:Molecular Sequence Data; D009119:Muscle Contraction / Q000187:drug effects*; D009842:Oligopeptides / Q000494:pharmacology; D007703:Peptidyl-Dipeptidase A / Q000494:pharmacology; D013373:Substance P / Q000494:pharmacology; D015244:Thiorphan / Q000494:pharmacology; D014132:Trachea / Q000187:drug effects* / Q000502:physiology
D009842:Oligopeptides; C064939:N-(1-carboxy-3-phenylpropyl)-alanyl-alanyl-phenylalanine-4-aminobenzoate; D013373:Substance P; D015244:Thiorphan; D007703:Peptidyl-Dipeptidase A; D008666:Metalloendopeptidases; C022510:thimet oligopeptidase; D001920:Bradykinin