Amplification of viral RNA for the detection of dengue types 1 and 2 virus.
In vitro DNA amplification by means of the polymerase chain reaction (PCR) was used to amplify dengue types 1 and 2 viral genomes in cultured cells and in the serum of persons infected with dengue virus. Results of the present investigation suggest that the PCR method is type-specific in detecting dengue virus and has a detection sensitivity of less than 100 plaque-forming units (pfu) for both serotypes of the virus. The PCR method may be useful for detecting and typing dengue virus in clinical and epidemiological specimens.
Department of Biochemistry, Chang Gung Medical College, Taipei, Taiwan, Republic of China.
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Pao
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Yao
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Lin
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King
D001483:Base Sequence; D015139:Blotting, Southern; D003716:Dengue Virus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D016679:Genome, Viral; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000235:genetics*; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D014158:Transcription, Genetic / Q000235:genetics
D012367:RNA, Viral; D012194:RNA-Directed DNA Polymerase