Enzyme-linked immunosorbent assays for detection of Pseudomonas fluorescens in sediment samples.
A strain-specific antibody to Pseudomonas fluorescens was used to develop two enzyme-linked immunosorbent assays: a sandwich and a competitive assay. Both assay types could be used to perform rapid and sensitive detection of the target organism in extracts of sediment samples.
Research Center for Microbiology, Water Quality Institute, Hørsholm, Denmark.
O
O
Nybroe
A
A
Johansen
M
M
Laake
D000907:Antibodies, Bacterial / Q000276:immunology; D000918:Antibody Specificity; D001667:Binding, Competitive; D004797:Enzyme-Linked Immunosorbent Assay; D011237:Predictive Value of Tests; D011551:Pseudomonas fluorescens / Q000276:immunology / Q000302:isolation & purification*; D015203:Reproducibility of Results; D012988:Soil Microbiology
D000907:Antibodies, Bacterial