The influence of low protein diet on the testicular toxicity of di(2-ethylhexyl)phthalate.
Oral administration of di(2-ethylhexyl)phthalate (DEHP) at 1000 mg/kg body weight to adult male albino rats maintained on low protein (LP) diet for 15 d resulted in a greater decrease in absolute and relative weights of the testis and in epididymal sperm count than in those rats maintained on a normal protein (NP) diet. A marked increase in the activity of testicular beta-glucuronidase and gamma-glutamyl transpeptidase (GGT) in the LP-fed animals suggested that LP diet enhanced the vulnerability of Sertoli cells towards DEHP. A greater decrease in the activity of testicular acid phosphatase, lactate dehydrogenase isoenzyme-X (LDH-X) and sorbitol dehydrogenase (SDH) in the LP-fed animals occurred in comparison to NP-fed animals. Degeneration of mature germinal cells in the LP-fed animals on exposure to DEHP suggested that LP diets enhance the susceptibility of the testis towards DEHP.
Tandon
R
R
Industrial Toxicology Research Centre, Mahatma Gandhi Marg, Lucknow, India.
Paramar
D
D
Singh
G B
GB
Seth
P K
PK
Srivastava
S P
SP
D000284:Administration, Oral; D000818:Animals; D004044:Dietary Proteins / Q000008:administration & dosage*; D004051:Diethylhexyl Phthalate / Q000008:administration & dosage / Q000633:toxicity*; D005966:Glucuronidase / Q000032:analysis; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013076:Sperm Count; D013094:Spermatozoa / Q000187:drug effects / Q000473:pathology; D013737:Testis / Q000187:drug effects* / Q000201:enzymology; D005723:gamma-Glutamyltransferase / Q000032:analysis
D004044:Dietary Proteins; D004051:Diethylhexyl Phthalate; D005723:gamma-Glutamyltransferase; D005966:Glucuronidase