The immune response to measles virus in mice. T-helper response to the nucleoprotein and mapping of the T-helper epitopes.
The T-helper response to the measles virus nucleoprotein (NP) has been studied in mice. The T-cell proliferative response was measured in lymphocytes from mice immunized with either a vaccinia measles-NP recombinant virus or a mouse neuro-adapted measles virus. A T-cell response was obtained with lymphocytes from H2d or H2k mice when stimulated with either measles virus or the NP expressed in bacteria. The response was CD4+ specific. The T-helper epitopes were mapped using truncated NP peptides. The major epitopes in both H2d and H2k mice were determined to be between amino acids 67-98. A further T-cell epitope (between amino acids 457-525) was identified when H2d mice were immunized with measles virus. Studies to quantitate the precursor cells for these epitopes confirmed that the region 67-98 of NP was immunodominant in both haplotypes immunized with the vaccinia-NP recombinant virus, whereas an additional major epitope was observed in the measles virus-infected H2d mice. The primary structure of the epitopes determined here are compared to predicted T-cell epitope motifs.
Giraudon
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P
Immunovirologie Moléculaire et Cellulaire, UMR 30-CNRS, Faculté de Médecine Alexis Carrel, Lyon, France.
Buckland
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R
Wild
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TF
D000595:Amino Acid Sequence; D000818:Animals; D002478:Cells, Cultured; D000939:Epitopes / Q000276:immunology*; D007111:Immunity, Cellular; D007114:Immunization; D008213:Lymphocyte Activation / Q000276:immunology; D008457:Measles / Q000276:immunology / Q000382:microbiology; D008459:Measles virus / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008808:Mice, Inbred CBA; D008969:Molecular Sequence Data; D019590:Nucleocapsid Proteins; D009698:Nucleoproteins / Q000276:immunology*; D011233:Precipitin Tests; D013154:Spleen / Q000166:cytology; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*; D014764:Viral Proteins / Q000276:immunology*
D000939:Epitopes; D019590:Nucleocapsid Proteins; D009698:Nucleoproteins; D014764:Viral Proteins; C058314:nucleoprotein, Measles virus