Localisation of the mitogenic epitope of staphylococcal enterotoxin B.
Limited digestion of staphylococcal enterotoxin B (SEB) with trypsin resulted in the generation of a 12-Kda amino-terminal fragment and a 17-Kda carboxy-terminal fragment which were isolated by preparative iso-electric focusing. The carboxy-terminal fragment exhibited significant mitogenic activity for murine splenocytes, whereas the isolated amino-terminal fragment possessed little detectable mitogenic activity. Monoclonal antibodies (MAbs) specific for the carboxy-terminal fragment neutralised most of the mitogenic activity of both the intact toxin and the carboxy-terminal fragment. MAbs specific for the amino-terminal fragment had no detectable neutralising activity. These results support the hypothesis that the epitope(s) responsible for mitogenic activity is located in the carboxy-terminal region of SEB.
Department of Microbiology and Immunology, Temple University School of Medicine, Philadelphia, PA 19140.
M
M
Binek
J R
JR
Newcomb
C M
CM
Rogers
T J
TJ
Rogers
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000918:Antibody Specificity; D015153:Blotting, Western; D002478:Cells, Cultured; D004591:Electrophoresis, Polyacrylamide Gel; D004768:Enterotoxins / Q000737:chemistry* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000032:analysis*; D006825:Hybridomas; D007525:Isoelectric Focusing; D008213:Lymphocyte Activation; D051379:Mice; D008934:Mitogens / Q000737:chemistry* / Q000276:immunology; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry* / Q000276:immunology; D013211:Staphylococcus aureus; D014357:Trypsin / Q000378:metabolism
D000911:Antibodies, Monoclonal; D004768:Enterotoxins; D000939:Epitopes; D008934:Mitogens; D010446:Peptide Fragments; C031818:enterotoxin B, staphylococcal; D014357:Trypsin