Regioselective modification of Lysine's amino groups by proteases.
The regioselectivity of six serine proteases for the amino groups of lysine was investigated. alpha-Chymotrypsin showed a preference for the alpha-amino group, although the selectivity can be varied 10-fold depending on the reaction medium. Subtilisin Carlsberg and other bacterial proteases were highly specific for the epsilon-amino group, regardless of the reaction medium: they were used as catalysts for the preparative synthesis of isopeptides in anhydrous tert-amyl alcohol.
Ashigara Research Laboratories, Fuji Photo Film Co., Ltd., Kanagawa, Japan.
H
H
Kitaguchi
M
M
Ono
I
I
Itoh
A M
AM
Klibanov
D000595:Amino Acid Sequence; D010450:Endopeptidases / Q000378:metabolism*; D008239:Lysine / Q000378:metabolism*; D008969:Molecular Sequence Data; D012997:Solvents
D012997:Solvents; D010450:Endopeptidases; D008239:Lysine