Glucose transporter expression and glucose utilization in skeletal muscle and brown adipose tissue during starvation and re-feeding.
Starvation (48 h) decreased the concentration of mRNA of the insulin-responsive glucose transporter isoform (GLUT 4) in interscapular brown adipose tissue (IBAT) (56%) and tibialis anterior (10%). Despite dramatic [7-fold (tibialis anterior) and 40-fold (IBAT)] increases in glucose utilization after 2 and 4 h of chow re-feeding, no significant changes in GLUT 4 mRNA concentration were observed in these tissues over this re-feeding period. The results exclude changes in GLUT 4 mRNA concentration in mediating the responses of glucose transport in these tissues to acute re-feeding after prolonged starvation.
Department of Medicine, Royal Postgraduate Medical School, Hammersmith Hospital, London, U.K.
D M
DM
Smith
S R
SR
Bloom
M C
MC
Sugden
M J
MJ
Holness
D002001:Adipose Tissue, Brown / Q000378:metabolism*; D000818:Animals; D015152:Blotting, Northern; D003847:Deoxyglucose / Q000378:metabolism*; D004435:Eating; D005260:Female; D005947:Glucose / Q000378:metabolism*; D009004:Monosaccharide Transport Proteins / Q000235:genetics / Q000378:metabolism*; D009132:Muscles / Q000378:metabolism*; D015345:Oligonucleotide Probes; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013217:Starvation / Q000378:metabolism*
D009004:Monosaccharide Transport Proteins; D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D012313:RNA; D003847:Deoxyglucose; D005947:Glucose