High-cell-density cultivation of Escherichia coli.
High-cell-density cultivations of Escherichia coli in glucose-mineral-salt media produce more than 100 g dry cells litre-1 in special fed-batch modes with feeding of glucose and ammonia only. The specific growth rate can be adjusted to allow optimum recombinant protein generation.
Riesenberg
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Institute of Microbiology and Experimental Therapy (ZIMET), Jena, Germany.
D001709:Biotechnology / Q000379:methods; D004926:Escherichia coli / Q000254:growth & development*