Genetic characterization of Legionella pneumophila serogroup 1 associated with respiratory disease in Australia.
Techniques were developed for genetic characterization of Legionella pneumophila serogroup 1 by using restriction fragment length polymorphism analysis. Allozyme analysis provided an index of the discrimination achieved by restriction fragment length polymorphism. Isolates from human cases of legionellosis were examined by both methods, and their profiles were compared with reference strains of L. pneumophila serogroup 1 obtained from the American Type Culture Collection. Eighteen distinct clones were evident among the isolates examined. Both methods could be used to trace the source of an outbreak of legionellosis caused by L. pneumophila serogroup 1.
Division of Clinical Microbiology, Institute of Medical and Veterinary Science, Adelaide, South Australia.
J
J
Lanser
M
M
Adams
R
R
Doyle
P
P
Hewitt
N
N
Sangster
D001315:Australia; D015139:Blotting, Southern; D003001:Cloning, Molecular; D006801:Humans; D016952:Legionella pneumophila / Q000145:classification / Q000235:genetics*; D007877:Legionnaires' Disease / Q000382:microbiology*; D012150:Polymorphism, Restriction Fragment Length; D012703:Serotyping