Affinity chromatography supports: a look at performance requirements.
Because of its high selectivity, affinity chromatography is a preferred tool in the downstream processing of high-value proteins and peptides of therapeutic interest. This review examines the affinity supports currently available, and investigates the performance characteristics and properties required of the support matrices for improved affinity-based supports for large-scale purification of biomolecules. Parameters for optimizing an affinity chromatographic process, and the advantages of affinity-based separation for scaled-up systems are highlighted.
Research and Development Laboratories, J. T. Baker Inc., Phillipsburg, New Jersey 08865.
S R
SR
Narayanan
L J
LJ
Crane
D002846:Chromatography, Affinity; D006801:Humans; D007164:Immunosorbents; D008024:Ligands; D010455:Peptides / Q000302:isolation & purification*; D011506:Proteins / Q000302:isolation & purification*; D012685:Sepharose; D012822:Silicon Dioxide
D007164:Immunosorbents; D008024:Ligands; D010455:Peptides; D011506:Proteins; D012822:Silicon Dioxide; D012685:Sepharose