Isolation and purification of human endometrial stromal and glandular cells using immunomagnetic microspheres.
Isolation of pure preparations of the different cell populations of human endometrium is a prerequisite for studies of in-vitro function. Sieving of dispersed endometrial cells, followed by adsorption onto immunomagnetic microspheres coated with antibody to Thy-1 was used to separate glandular and stromal cells. The purity of these cell populations was checked with antibodies to cytokeratin and Thy-1. The stromal cells were 98% pure and 90% viable, gland cells were 82% pure with 76% viability. The purified cells were able to proliferate in vitro as shown by thymidine incorporation.
University of Oxford, Nuffield Department of Obstetrics and Gynaecology, John Radcliffe Hospital, Headington, UK.
S
S
Fernández-Shaw
S C
SC
Shorter
C E
CE
Naish
D H
DH
Barlow
P M
PM
Starkey
D000954:Antigens, Surface / Q000276:immunology; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D002498:Centrifugation; D004717:Endometrium / Q000166:cytology / Q000276:immunology; D004847:Epithelial Cells; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008280:Magnetics; D008562:Membrane Glycoproteins / Q000276:immunology; D008863:Microspheres; D018800:Thy-1 Antigens; D013936:Thymidine / Q000378:metabolism
D000954:Antigens, Surface; D008562:Membrane Glycoproteins; D018800:Thy-1 Antigens; D013936:Thymidine