Production of benzoylformic acid from phenylglycine by Saccharomycopsis lipolytica.
Microbial production of benzoylformic acid (BF), which can be used as a substrate of enzymatic synthesis of (R)-(-)-mandelic acid, was investigated. Among 145 strains of yeasts and actinomycetes, Saccharomycopsis lipolytica (IAM 4964) was the best producer of BF from DL-phenylglycine (DL-PG). Culture conditions for BF production by the organism were optimized. When 0.2% fructose as a carbon source and 0.7% Bacto-tryptone as a nitrogen source were used in the presence of 4% DL-PG, 14.5 mg/ml of BF was produced (about 37% molar yield) in 4 days of cultivation. BF was synthesized from the L-form of PG, but not from the D-form. The BF was isolated from culture broth in a crystalline form and physicochemically identified.
Department of Agricultural Chemistry, Kyoto University, Japan.
H
H
Kanzaki
A
A
Isobe
Y
Y
Izumi
H
H
Yamada
D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000378:metabolism*; D005632:Fructose / Q000494:pharmacology; D005947:Glucose / Q000494:pharmacology; D005990:Glycerol / Q000494:pharmacology; D005998:Glycine / Q000031:analogs & derivatives* / Q000378:metabolism; D006038:Glyoxylates / Q000302:isolation & purification / Q000378:metabolism*; D007700:Kinetics; D008333:Mandelic Acids; D009584:Nitrogen / Q000494:pharmacology; D012442:Saccharomycopsis / Q000378:metabolism*; D013237:Stereoisomerism
D000894:Anti-Inflammatory Agents, Non-Steroidal; D006038:Glyoxylates; D008333:Mandelic Acids; C012482:phenylglyoxylic acid; D005632:Fructose; C008852:2-phenylglycine; D005947:Glucose; D009584:Nitrogen; D005990:Glycerol; D005998:Glycine