Flow-induced release of adenosine 5'-triphosphate from endothelial cells of the rat mesenteric arterial bed.
Adenosine 5'-triphosphate (ATP) was released into the perfusate of rat isolated mesenteric arterial beds during each of two consecutive increases in flow. There was no significant difference between the amounts of ATP released on each occasion. Substance P was also released into the perfusate by increased flow, although its release was more variable. Removal of the endothelium of the mesenteric vessels with sodium deoxycholate led to a significant reduction (74%) in the amount of ATP released compared with the release before the endothelium had been removed. This suggests that the ATP released into the mesenteric arterial perfusate during increased flow arises from endothelial cells.
Department of Anatomy and Developmental Biology, University College London, England.
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Ralevic
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Milner
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Kirkpatrick
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Burnstock
D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D001783:Blood Flow Velocity; D004730:Endothelium, Vascular / Q000378:metabolism*; D066298:In Vitro Techniques; D008297:Male; D008638:Mesenteric Arteries / Q000378:metabolism*; D010477:Perfusion; D051381:Rats; D011919:Rats, Inbred Strains; D013152:Splanchnic Circulation / Q000502:physiology*; D013373:Substance P / Q000378:metabolism
D013373:Substance P; D000255:Adenosine Triphosphate