HLA class II frequencies in celiac disease patients in the west of Ireland.
Restriction fragment length polymorphism analysis, using a single restriction enzyme TaqI-multiple-probe system for HLA-DRB1-DQB1 and -DQA1, was used to determine HLA-DR and -DQ frequencies in 56 unrelated celiac patients and 47 unrelated controls from the west of Ireland. In addition, HLA-DPB1 allelic frequencies were determined in the same group of patients and controls by using the technique of enzymatic DNA amplification of the polymorphic second exon of HLA-DPB1 genes in conjunction with sequence-specific oligonucleotide probing. The results suggest that HLA-DQ rather than HLA-DR is more important in conferring susceptibility to celiac disease. Furthermore, no association between HLA-DP and celiac disease was found in this study.
Northern Ireland Tissue Typing Service, Belfast City Hospital.
D A
DA
Savage
D
D
Middleton
F
F
Trainor
A
A
Taylor
M
M
Carson
F M
FM
Stevens
C F
CF
McCarthy
D000293:Adolescent; D000328:Adult; D000483:Alleles; D002446:Celiac Disease / Q000453:epidemiology / Q000235:genetics* / Q000276:immunology; D002648:Child; D005787:Gene Frequency; D005802:Genes, MHC Class II; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006683:HLA-DQ Antigens / Q000235:genetics*; D006684:HLA-DR Antigens / Q000235:genetics*; D015803:HLA-DR3 Antigen / Q000235:genetics; D006239:Haplotypes; D006801:Humans; D007494:Ireland / Q000453:epidemiology; D012150:Polymorphism, Restriction Fragment Length
D006683:HLA-DQ Antigens; D006684:HLA-DR Antigens; D015803:HLA-DR3 Antigen