Participation of the CD27 antigen in the regulation of IL-2-activated human natural killer cells.
The CD27 Ag is expressed by the majority of resting T lymphocytes and appears to play a crucial role in T cell activation. We found that some resting peripheral blood NK cells also express CD27. Furthermore, CD27 expression was up-regulated on NK cells stimulated by IL-2. The cytolytic activity of IL-2-activated, but not resting, NK cells was inhibited by an anti-CD27 mAb (anti-1A4). However, anti-1A4 did not affect conjugate formation between IL-2-activated NK cells and tumor cell targets. In contrast, anti-1A4 inhibited CD2-mediated calcium mobilization and the serine esterase activity of NK cell granules. These inhibitory effects could be mediated in part by increase in intracellular cAMP levels induced by anti-1A4. Our results suggest that the CD27 Ag plays an important role in the regulation of activated NK cells.
Division of Tumor Immunology, Dana-Farber Cancer Institute, Boston, MA.
K
K
Sugita
M J
MJ
Robertson
Y
Y
Torimoto
J
J
Ritz
S F
SF
Schlossman
C
C
Morimoto
D015703:Antigens, CD / Q000276:immunology*; D000943:Antigens, Differentiation / Q000502:physiology; D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology* / Q000502:physiology; D018801:CD2 Antigens; D002118:Calcium / Q000502:physiology; D000242:Cyclic AMP / Q000502:physiology; D003602:Cytotoxicity, Immunologic; D006801:Humans; D066298:In Vitro Techniques; D007376:Interleukin-2 / Q000494:pharmacology*; D015979:Killer Cells, Lymphokine-Activated / Q000276:immunology; D007694:Killer Cells, Natural / Q000276:immunology*; D008213:Lymphocyte Activation; D011961:Receptors, Fc / Q000502:physiology; D017452:Receptors, IgG; D011971:Receptors, Immunologic / Q000502:physiology; D013869:Thiolester Hydrolases / Q000378:metabolism; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7
D015703:Antigens, CD; D000943:Antigens, Differentiation; D000945:Antigens, Differentiation, T-Lymphocyte; D018801:CD2 Antigens; D007376:Interleukin-2; D011961:Receptors, Fc; D017452:Receptors, IgG; D011971:Receptors, Immunologic; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7; D000242:Cyclic AMP; D013869:Thiolester Hydrolases; D002118:Calcium