Primary structure analysis of polypeptides.
Primary structure determination together with other techniques such as amino acid analysis, peptide mapping, and polyacrylamide gel electrophoresis provide a physical characterisation of a polypeptide. This has been valuable in the research field for some time, but is now being applied to problems which arise in the areas of process development, formulation and QA of protein products. Probably the most significant contribution is elucidation of processing events, since this may go undetected by other techniques. Thus, with the emergence of various protein products from biotechnology, a technique which has been of great value in research activities for many years is finding its place in the range of techniques used for process development, formulation and QA.
Smith
B J
BJ
Celltech Ltd, Slough, Berkshire, UK.
D000595:Amino Acid Sequence; D001709:Biotechnology; D015394:Molecular Structure; D010455:Peptides / Q000737:chemistry*
D010455:Peptides