Comparison of insulin-like growth factor interaction with satellite cells and embryonic myoblasts derived from the turkey.
1. The interaction of insulin-like growth factors (IGFs) with receptors on clonal-derived turkey satellite cells and embryonic myoblasts was compared using competitive binding assays and affinity cross-linking analysis. 2. Although [125I]IGF-I and [125I]IGF-II were displaced similarly by IGF-I and IGF-II within cell lines (P greater than 0.05), displacement, and therefore dissociation constants, differed between cell lines (P less than 0.0001). 3. Receptor cross-linking analysis using iodinated IGFs suggests that both IGF-I and IGF-II interact with the type I receptor on turkey embryonic and posthatch myogenic cells.
Department of Animal and Range Science, South Dakota State University, Brookings 57007-0392.
S S
SS
Sun
D C
DC
McFarland
N H
NH
Ferrin
K K
KK
Gilkerson
D000818:Animals; D001345:Autoradiography; D001667:Binding, Competitive; D002478:Cells, Cultured; D003432:Cross-Linking Reagents; D004591:Electrophoresis, Polyacrylamide Gel; D008297:Male; D009132:Muscles / Q000166:cytology / Q000196:embryology / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000378:metabolism; D017451:Receptors, Somatomedin; D013002:Somatomedins / Q000378:metabolism*; D014422:Turkeys / Q000196:embryology / Q000378:metabolism*
D003432:Cross-Linking Reagents; D011956:Receptors, Cell Surface; D017451:Receptors, Somatomedin; D013002:Somatomedins