Degradation products of the mRNA encoding the small subunit of ribulose-1,5-bisphosphate carboxylase in soybean and transgenic petunia.
The degradation of a soybean ribulose-1,5-bisphosphate carboxylase small subunit RNA, SRS4, was investigated in soybean seedlings and in petunia plants transformed with an SRS4 gene construct. Polyacrylamide RNA gel blot, primer extension, and S1 nuclease analyses were used to identify and map fragments of the SRS4 mRNA generated in vivo. We showed that SRS4 mRNA is degraded to a characteristic set of fragments in soybean and transgenic petunia and that degradation is not dependent on position of insertion of the gene construct within the genome, on the expression level of the SRS4 mRNA, or on the rbcS promoter. Degradation products lacked poly(A) tails and fractionated with poly(A)-depleted RNA on oligo(dT)-sepharose columns. These products pelleted with polysomes and were released from polysomes prepared with EDTA. Sequences at the 5' end of the SRS4 mRNA were more stable than those at the 3' end of the mRNA. Three models for SRS4 mRNA degradation involving endonucleolytic and exonucleolytic degradation were presented to explain the origin of the 5' proximal fragments.
Thompson
D M
DM
Department of Genetics, University of Georgia, Athens 30602.
Tanzer
M M
MM
Meagher
R B
RB
D001483:Base Sequence; D004492:Edetic Acid / Q000494:pharmacology; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes; D010944:Plants / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000235:genetics; D011061:Poly A / Q000378:metabolism; D011132:Polyribosomes / Q000187:drug effects / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D012273:Ribulose-Bisphosphate Carboxylase / Q000235:genetics*; D013025:Glycine max / Q000378:metabolism
D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D011061:Poly A; D004492:Edetic Acid; D012273:Ribulose-Bisphosphate Carboxylase