Structural study of cell wall mannan of a Candida albicans (serotype A) strain.
The structure of the mannan of Candida albicans NIH A-207 strain (serotype A) was investigated by adopting mild acetolysis followed by enzymolysis with an Arthrobacter GJM-1 exo-alpha-mannosidase. The resultant oligosaccharides, from pentaose to octaose (where manp = D-mannopyranose), were identified as manp beta (1----2)manp alpha (1----2)manp alpha (1----2)manp alpha (1----2)manp, manp beta (1----2)manp beta (1----2)manp alpha (1----2)manp alpha (1----2)- manp alpha (1----2)manp, manp beta (1----2)manp beta (1----2)manp beta (1----2)manp alpha (1----2)manp alpha (1----2)manp alpha (1----2)manp and manp beta (1----2)manp beta (1----2)manp beta (1----2)manp beta (1----2)manp alpha (1----2)manp alpha (1----2)manp alpha (1----2)manp, respectively. Analyses of alpha-linked oligosaccharides obtained by acetolysis under conventional conditions gave the same oligosaccharides, from biose to heptaose, as those obtained from the mannans of C. albicans NIH B-792 (serotype B) and J-1012 (serotype A, formerly serotype C).
Second Department of Hygienic Chemistry, Tohoku College of Pharmacy, Miyagi, Japan.
H
H
Kobayashi
N
N
Shibata
T
T
Osaka
Y
Y
Miyagawa
Y
Y
Ohkubo
S
S
Suzuki
D002176:Candida albicans / Q000737:chemistry*; D002240:Carbohydrate Sequence; D002473:Cell Wall / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D008351:Mannans / Q000737:chemistry*; D008969:Molecular Sequence Data; D015394:Molecular Structure; D013045:Species Specificity
D008351:Mannans