Characterization of the myeloid-specific CD11b promoter.
The CD11b/CD18 heterodimeric surface antigen is expressed exclusively on human monocytes, macrophages, granulocytes, and natural killer cells. During differentiation of myeloid cell lines, CD11b steady state messenger RNA levels increase significantly; we show here that CD11b transcription rates increase commensurately. A 1.7-kb fragment of CD11b 5' flanking sequence directs expression of a reporter gene specifically in myeloid cell lines. Deletion analysis localizes elements directing high levels of tissue-specific reporter gene expression to the 412 bp proximal to the transcriptional start site. This sequence contains two consensus binding sites for Sp1, a GATA motif, and a purine-rich sequence that presents potential binding sites for members of the ets family of genes. Analysis of this promoter should result in the isolation of myeloid-specific transcription factors and the development of methods to direct the myeloid-specific expression of heterologous genes.
Hematology/Oncology Division, Beth Israel Hospital, Boston, MA 02215.
H L
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Pahl
A G
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D015703:Antigens, CD / Q000235:genetics*; D001483:Base Sequence; D015152:Blotting, Northern; D018845:CD11 Antigens; D002460:Cell Line; D004247:DNA / Q000737:chemistry; D015870:Gene Expression; D005786:Gene Expression Regulation; D006098:Granulocytes / Q000166:cytology / Q000276:immunology*; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics; D014158:Transcription, Genetic; D014162:Transfection
D015703:Antigens, CD; D018845:CD11 Antigens; D012333:RNA, Messenger; D004247:DNA