Use of mammalian cell expression cloning systems to identify genes for cytokines, receptors, and regulatory proteins.
Mammalian cell expression cloning has become a standard technique for the isolation of mammalian genes or cDNAs. Its advantage is that the biological functions of the gene of interest are used for cloning. Therefore, the identified cDNAs or genes should be functional in vivo, and there is no need for physical or chemical information about the gene products, so that protein purification in sufficient quantity to raise antibodies or to obtain amino acid sequences is not necessary. Here, we summarize recent progress in mammalian cell cloning systems, and discuss the possible directions in which this technique will lead.
Institute of Medical Science, University of Tokyo, Japan.
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Nakayama
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Yokota
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Arai
D000818:Animals; D001709:Biotechnology; D002460:Cell Line; D003001:Cloning, Molecular / Q000379:methods*; D016207:Cytokines / Q000235:genetics; D004247:DNA / Q000235:genetics; D015870:Gene Expression; D005818:Genetic Engineering; D005822:Genetic Vectors; D006801:Humans; D010957:Plasmids; D011956:Receptors, Cell Surface / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics
D016207:Cytokines; D011956:Receptors, Cell Surface; D011994:Recombinant Proteins; D004247:DNA