A Drosophila metallothionein promoter is inducible in mosquito cells.
Expression from a Drosophila metallothionein promoter (Mtn) was investigated in mosquito cells. Recombinant plasmids carrying a transcription unit comprised of the Escherichia coli beta-galactosidase gene (lacZ) fused to the metallothionein promoter were stably introduced into Aedes albopictus C6/36 cells. A low copy transformant containing approximately 60 copies of plasmid per cell, and a high copy transformant (1-2 x 10(4) copies/cell) were characterized. The expression of beta-galactosidase from the metallothionein promoter could be induced and controlled in this heterologous system, even when the copy number of introduced plasmid was several thousand.
Department of Microbiology, Colorado State University, Fort Collins 80526.
M J
MJ
Kovach
J O
JO
Carlson
B J
BJ
Beaty
D000330:Aedes / Q000737:chemistry / Q000235:genetics*; D000818:Animals; D002460:Cell Line; D003001:Cloning, Molecular; D004331:Drosophila melanogaster / Q000235:genetics*; D005786:Gene Expression Regulation; D005822:Genetic Vectors; D008668:Metallothionein / Q000235:genetics* / Q000302:isolation & purification; D011401:Promoter Regions, Genetic; D001616:beta-Galactosidase / Q000235:genetics
D008668:Metallothionein; D001616:beta-Galactosidase