Physiology of myeloma cells grown in glucose-limited chemostat cultures.
A recombinant myeloma NS1-derived clone was grown in chemostat cultures in Dulbecco's MEM/Ham's F12 (1:1) medium containing various concentrations of glucose, at a dilution rate of 0.028 h-1. Serum-supplemented cultures were virtually glucose-limited at a large range of glucose feed concentrations (0.7-5 mM). True glucose-limited cultures, however, were only established at low glucose supply levels to 1.3 mM at a maximum. In cultures obtained at higher glucose concentrations methionine was shown to be the growth-limiting compound. The pattern derived for serum-free chemostat cultures was similar, except that growth yields on glucose were much lower. Glucose was shown to be the growth-limiting substrate in cultures fed with media containing less than 4.5 mM glucose. Upon supplying glucose at higher concentrations such cultures presumably run into methionine and/or tryptophan limitation.
Department of Biotechnology and Biochemistry, Organon International BV, Oss, The Netherlands.
P R
PR
Levering
J A
JA
van Heijst
C M
CM
Sünnen
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001790:Blood Physiological Phenomena; D003470:Culture Media; D016895:Culture Media, Serum-Free; D005947:Glucose / Q000378:metabolism*; D051379:Mice; D009101:Multiple Myeloma / Q000503:physiopathology*; D014407:Tumor Cells, Cultured
D000596:Amino Acids; D003470:Culture Media; D016895:Culture Media, Serum-Free; D005947:Glucose