In vivo identification and monitoring of changes in rat brain glucose by two-dimensional shift-correlated 1H NMR spectroscopy.
Intracerebral glucose resonance was directly detected and resolved in vivo by two-dimensional shift-correlated (COSY) 1H NMR spectroscopy in anesthetized rats (n = 4). The relative changes in brain glucose concentration were measured by volume integration of the alpha-D-glucose cross peak in the 2D COSY spectra. This report demonstrates the possibility of monitoring the variations in cerebral glucose following iv injection of glucose.
Peres
M
M
Institut de Chimie des Substances Naturelles C.N.R.S., Gif sur Yvette, France.
Fedeli
O
O
Barrere
B
B
Gillet
B
B
Berenger
G
G
Seylaz
J
J
Beloeil
J C
JC
D000818:Animals; D001224:Aspartic Acid / Q000031:analogs & derivatives / Q000378:metabolism; D001786:Blood Glucose / Q000032:analysis; D001921:Brain / Q000378:metabolism*; D005947:Glucose / Q000008:administration & dosage / Q000378:metabolism*; D005971:Glutamates / Q000378:metabolism; D018698:Glutamic Acid; D005973:Glutamine / Q000378:metabolism; D006859:Hydrogen; D007294:Inositol / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008297:Male; D008991:Monitoring, Physiologic; D051381:Rats; D017208:Rats, Wistar
D001786:Blood Glucose; D005971:Glutamates; D005973:Glutamine; D001224:Aspartic Acid; D018698:Glutamic Acid; D007294:Inositol; D006859:Hydrogen; C000179:N-acetylaspartate; D005947:Glucose