Quantification of Trypanosoma cruzi in the heart, lymph nodes and liver of experimentally infected mice, using limiting dilution analysis.
Limiting dilution analysis was used to quantify Trypanosoma cruzi in the lymph nodes, liver and heart of Swiss and C57Bl/10 mice. The results showed that, in Swiss and Bl/10 mice infected with T. cruzi Y strain, the number of parasites/mg of tissue increased during the course of the infection in both types of mice, although a greater number of parasites were observed in heart tissue from Swiss mice than from Bl/10. With regard to liver tissue, it was observed that the parasite load in the initial phase of infection was higher than in heart. In experiments using T. cruzi Colombian strain, the parasite load in the heart of Swiss and Bl/10 mice increased relatively slowly, although high levels of parasitization were nonetheless observable by the end of the infection. As for the liver and lymph nodes, the concentration of parasites was lower over the entire course of infection than in heart. Both strains thus maintained their characteristic tissue tropisms. The limiting dilution assay (LDA) proved to be an appropriate method for more precise quantification of T. cruzi, comparing favorably with other direct microscopic methods that only give approximate scores.
Departamento de Protozoologia, Instituto Oswaldo Cruz, Rio de Janeiro, Brasil.
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D000818:Animals; D014355:Chagas Disease / Q000469:parasitology*; D006321:Heart / Q000469:parasitology*; D008099:Liver / Q000469:parasitology*; D008198:Lymph Nodes / Q000469:parasitology*; D008297:Male; D051379:Mice / Q000145:classification / Q000469:parasitology; D008810:Mice, Inbred C57BL; D013045:Species Specificity; D014349:Trypanosoma cruzi / Q000145:classification / Q000302:isolation & purification*