The role of egg antigens, cytokines in granuloma formation in murine schistosomiasis mansoni.
The induction of granuloma formation by soluble egg antigens (SEA) of Schistosoma mansoni is accompanied by T cell-mediated lymphokine production that regulates the intensity of the response. In the present study we have examined the ability of SDS-PAGE fractionated SEA proteins to elicit granulomas and lymphokine production in infected and egg-immunized mice. At the acute stage of infection SEA fractions (< 21, 25-30, 32-38, 60-66, 70-90, 93-125, and > 200 kD) that elicited pulmonary granulomas also elicited IL-2, IL-4 lymphokine production. At the chronic stage a diminished number of fractions (60-66, 70-90, 93-125, and > 200 kD) were able to elicit granulomas with an overall decrease in IL-2, IL-4 production. Granulomas were elicited by larval-egg crossreactive and egg-specific fractions at both the acute and chronic stage of the infection. Examination of lymphokine production from egg-immunized mice demonstrated that as early as 4 days IL-2 was produced by spleen cells stimulated with < 21, 32-38, 40-46, 93-125, and > 200 kD fractions. By 16 days, IL-2 production was evoked by 8 of 9 fractions. IL-4 production at 4 days in response to all fractions was minimal while at 16 days IL-4 was elicited with the < 21, 25-30, 50-56, 93-125, and > 200 kD fractions. The present study reveals differences in the range of SEA fractions able to elicit granulomas and IL-2, IL-4 production between acute and chronic stages of infection.(ABSTRACT TRUNCATED AT 250 WORDS)
Department of Immunology/Microbiology, Wayne State University, School of Medicine, Detroit, MI 48201.
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Boros
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Lukacs
D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology* / Q000302:isolation & purification / Q000633:toxicity; D016207:Cytokines / Q000502:physiology*; D004527:Egg Proteins / Q000276:immunology / Q000302:isolation & purification / Q000633:toxicity; D005260:Female; D006099:Granuloma / Q000276:immunology* / Q000469:parasitology / Q000503:physiopathology; D015801:Helminth Proteins / Q000276:immunology / Q000302:isolation & purification / Q000633:toxicity; D007376:Interleukin-2 / Q000096:biosynthesis; D015847:Interleukin-4 / Q000096:biosynthesis; D008222:Lymphokines / Q000502:physiology; D051379:Mice; D008808:Mice, Inbred CBA; D010063:Ovum / Q000276:immunology*; D012550:Schistosoma mansoni / Q000276:immunology*; D012555:Schistosomiasis mansoni / Q000276:immunology* / Q000503:physiopathology; D013154:Spleen / Q000276:immunology; D016176:T-Lymphocyte Subsets / Q000276:immunology
D000947:Antigens, Helminth; D016207:Cytokines; D004527:Egg Proteins; D015801:Helminth Proteins; D007376:Interleukin-2; D008222:Lymphokines; D015847:Interleukin-4