Flavonol glycoside production in callus cultures of Epimedium diphyllum.
Callus cultures of Epimedium diphyllum produced a large amount of epimedoside A in addition to a small amount of diphylloside B, ikarisoside C, epimedoside E, diglycosides of des-O-methylanhydroicaritin (8-gamma, gamma-dimethylallylkaempfero). Icariin, epimedins A-C, which are glycosides of anhydroicaritin, were also produced in the callus cultures. Contents of the flavonol glycosides in callus tissue were higher than those of mother plants, but the composition of each flavonol glycoside mixture in the callus cultures was different from that of the original plants. The time-course experiments showed that an inverse relationship existed between cell growth and flavonol glycoside production. Effects of hormonal factors on cell growth and flavonol glycoside production indicated that 2,4-dichlorophenoxyacetic acid was needed for the production of flavonol glycosides.
Department of Pharmacognosy, Gifu Pharmaceutical University, Japan.
H
H
Yamamoto
K
K
Ieda
S
S
Tsuchiya
K
K
Yan
T
T
Tanaka
M
M
Iinuma
M
M
Mizuno
D002240:Carbohydrate Sequence; D046508:Culture Techniques; D005419:Flavonoids / Q000096:biosynthesis* / Q000737:chemistry; D008969:Molecular Sequence Data; D015394:Molecular Structure; D010937:Plant Growth Regulators / Q000494:pharmacology; D010944:Plants
D005419:Flavonoids; D010937:Plant Growth Regulators