Sequence and embryonic expression of the murine Hox-3.5 gene.
The murine Hox-3.5 gene has been mapped and linked genomically to a position 18 kb 3' of its most 5' locus neighbour, Hox-3.4, on chromosome 15. The sequence of the Hox-3.5 cDNA, together with the position of the gene within the locus, show it to be a paralogue of Hox-2.6, Hox-1.4 and Hox-4.2. The patterns of embryonic expression for the Hox-3.5 gene are examined in terms of three rules, proposed to relate a Hox gene's expression pattern to its position within the locus. The anterior boundaries of Hox-3.5 expression in the hindbrain and prevertebral column lie anterior to those of Hox-3.4 and all other, more 5'-located Hox-3 genes. Within the hindbrain, the Hox-3.5 boundary is seen to lie posterior to that of its paralogue, Hox-2.6, by a distance equal to about the length of one rhombomere. Patterns of Hox-3.5 expression within the oesophagus and spinal cord, but not the testis, are similar to those of other Hox-3 genes, Hox-3.3 and Hox-3.4.
Department of Cell and Structural Biology, University of Manchester, UK.
A M
AM
Geada
S J
SJ
Gaunt
M
M
Azzawi
S M
SM
Shimeld
J
J
Pearce
P T
PT
Sharpe
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D005314:Embryonic and Fetal Development / Q000235:genetics*; D004947:Esophagus / Q000196:embryology; D015870:Gene Expression / Q000235:genetics*; D005801:Genes, Homeobox / Q000235:genetics*; D017404:In Situ Hybridization, Fluorescence; D051379:Mice; D008969:Molecular Sequence Data; D012249:Rhombencephalon / Q000196:embryology; D017386:Sequence Homology, Amino Acid; D013116:Spinal Cord / Q000196:embryology