Expression of p53 gene in B-CLL peripheral lymphocytes.
The p53 tumor suppressor gene expression has been studied in 23 B-CLL cases at different clinical stages. The analysis failed to show a direct correlation with each stage, but the significantly lower frequency of a BglII RFLP in the pathologic population suggests a role of this gene in B-CLL. Northern Blot analysis showed the expression of p53 mRNA in all the B-CLL cases. A protocol for the RT-PCR methods was set up to study a very small amount of materials which should be better used for sequence analysis.
Istituto di Chimica Biologica, Centro di Studi Biochimici delle Patologie del Genoma Umano.
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D004273:DNA, Neoplasm / Q000235:genetics; D005787:Gene Frequency; D016158:Genes, p53; D006801:Humans; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000235:genetics*; D008214:Lymphocytes / Q000737:chemistry; D012150:Polymorphism, Restriction Fragment Length
D004273:DNA, Neoplasm