Establishment and characterization of monoclonal antibodies against bovine ephemeral fever virus.
We established fourteen monoclonal antibodies (MAbs) reactive to bovine ephemeral fever virus YHL strain, and characterized six representatives including three IgG1s (YG3/4, YG5/8, and YG6/7) and three IgMs (YM4/9, YM2/6, and YM6/8). Among them, YG3/4 and YM4/9 gave especially strong reactivities to the virus. YM4/9 reacted specifically with a 43K antigen of the virus, corresponding to the matrix protein 1. The other MAbs reacted most strongly with the 43K antigen, but also reacted with unknown 23K and 21K antigens. By a simultaneous two-site method using YM4/9 and YG3/4, it was possible to detect 10(4.10)TCID50/ml of the virus, in the presence of serum.
Eto
N
N
Department of Food Science and Technology, Faculty of Agriculture, Kyushu University, Fukuoka, Japan.
Yamada
K
K
Koga
A
A
Shirahata
S
S
Murakami
H
H
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000914:Antibodies, Viral / Q000276:immunology*; D000956:Antigens, Viral / Q000032:analysis; D015153:Blotting, Western / Q000662:veterinary; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D004810:Ephemeral Fever / Q000382:microbiology*; D005455:Fluorescent Antibody Technique / Q000662:veterinary; D009500:Neutralization Tests / Q000662:veterinary; D012209:Rhabdoviridae / Q000276:immunology* / Q000302:isolation & purification
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral