Continuous production of large amounts of monoclonal immunoglobulins in hollow fibers using protein-free medium.
The performance of a protein-free medium was compared in culture flasks with a serum-supplemented medium and with a serum free medium in terms of cell growth and monoclonal antibody production by a murine hybridoma. We present results of continuous production in hollow fiber culture systems using serum-free medium and protein-free medium. In protein-free medium, it has been possible to produce large quantities of monoclonal antibody with a productivity similar to that obtained in serum-free medium. After a two steps purification process, monoclonal antibodies were characterized by SDS-PAGE, High Performance Size Exclusion Chromatography and Free Solution Capillary Electrophoresis. SDS-PAGE and high performance chromatography analysis have showed that purified monoclonal antibodies produced in serum-free medium or protein-free medium were similar. Furthermore, Capillary Electrophoresis characterization revealed that both MAbs were constituted by three isoforms with equivalent electrophoretic mobilities.
Dhainaut
F
F
Innovation Therapeutique, CNTS 3, Les Ulis, France.
Bihoreau
N
N
Meterreau
J L
JL
Lirochon
J
J
Vincentelli
R
R
Mignot
G
G
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000302:isolation & purification; D002452:Cell Count; D002455:Cell Division; D002850:Chromatography, Gel; D016895:Culture Media, Serum-Free; D046508:Culture Techniques / Q000295:instrumentation / Q000379:methods; D006825:Hybridomas; D007074:Immunoglobulin G / Q000096:biosynthesis* / Q000302:isolation & purification; D007700:Kinetics; D051379:Mice; D010101:Oxygen Consumption; D013997:Time Factors
D000911:Antibodies, Monoclonal; D016895:Culture Media, Serum-Free; D007074:Immunoglobulin G