pH-dependent flagella formation by facultative alkaliphilic Bacillus sp. C-125.
A facultative alkaliphilic strain of Bacillus sp. C-125 grown at alkaline pH had many sinuous peritrichous flagella and was highly motile. However, most of the cells grown initially at pH 7 were non-motile and possessed few straight flagella. The amount of flagellin was low when the organism was grown at pH 7, suggesting that non-motility is due to poor synthesis of flagellin. The molecular mass of the flagellin was 37 kDa and the isoelectric point was pH 5.0. The amino acid composition of the flagellin was similar to that found in the flagellin from neutrophilic Bacillus subtilis 168.
Department of Bioengineering, Faculty of bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Japan.
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R
Aono
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Ogino
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Horikoshi
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D001407:Bacillus / Q000254:growth & development* / Q000378:metabolism / Q000648:ultrastructure; D005407:Flagella / Q000378:metabolism* / Q000648:ultrastructure; D005408:Flagellin / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification; D006863:Hydrogen-Ion Concentration; D007526:Isoelectric Point; D008969:Molecular Sequence Data; D008970:Molecular Weight
D000596:Amino Acids; D005408:Flagellin