Highly inducible synthesis of heterologous proteins in epithelial cells carrying a glucocorticoid-responsive vector.
A glucocorticoid-responsive vector is described which allows for the highly inducible expression of complementary DNAs (cDNAs) in stably transfected mammalian cell lines. This vector, pLK-neo, composed of a variant mouse mammary tumor virus long terminal repeat promoter, containing a hormone regulatory element, a Geneticin resistance-encoding gene in a simian virus 40 transcription unit, and a polylinker insertion site for heterologous cDNAs, was used to express the polymeric immunoglobulin (poly-Ig) receptor and the thymocyte marker, Thy-1, in Madin-Darby canine kidney (MDCK) cells and in murine fibroblast L cells. A high level of poly-Ig receptor or Thy-1 mRNA accumulation was observed in MDCK cells in response to dexamethasone with a parallel ten- to 200-fold increase in protein synthesis depending on the recombinant protein and the transfected cell clone.
Institute of Biochemistry, University of Lausanne, Epalinges, Switzerland.
R P
RP
Hirt
O
O
Poulain-Godefroy
J
J
Billotte
J P
JP
Kraehenbuhl
N
N
Fasel
D000818:Animals; D000954:Antigens, Surface / Q000235:genetics; D002460:Cell Line; D003907:Dexamethasone / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects*; D005822:Genetic Vectors / Q000235:genetics*; D008324:Mammary Tumor Virus, Mouse / Q000235:genetics*; D008562:Membrane Glycoproteins / Q000235:genetics; D051379:Mice; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*; D012635:Secretory Component / Q000235:genetics; D018800:Thy-1 Antigens; D014162:Transfection / Q000235:genetics
D000954:Antigens, Surface; D008562:Membrane Glycoproteins; D011994:Recombinant Proteins; D012635:Secretory Component; D018800:Thy-1 Antigens; D003907:Dexamethasone