Superoxide dismutase from Trichuris ovis--inhibition by benzimidazoles and pyrimidine derivatives.
Three superoxide dismutase isoenzymes of different cellular location were detected in an homogenate of Trichuris ovis. Each of these molecular forms was purified by differential centrifugation and precipitation with ammonium sulphate, followed by chromatography on DEAE-cellulose and Sephadex G-75 columns. The activity levels of the two molecular forms detected in the mitochondrial (one cyanide sensitive Cu-Zn-SOD and the other cyanide insensitive Mn-SOD) were higher than that of the superoxide dismutase detected in the cytoplasmic fraction (cyanide sensitive Cu-Zn-SOD). All molecular forms present evident differences to the SODs contained in the host liver. Molecular mass and some of the physical and chemical properties of the enzyme was determined for all three molecular forms. An inhibitory effect on the SOD of the parasite an the host was detected with a series of compounds, some of which markedly inhibited parasite enzyme but not host enzyme.
Sanchez-Moreno
M
M
Departamento de Bioquímica y Biologia Molecular, Facultad de Ciencias, Universidad de Granada, España.
Garcia-Rejon
L
L
Salas
I
I
Osuna
A
A
Monteoliva
M
M
D000818:Animals; D001562:Benzimidazoles / Q000494:pharmacology; D002848:Chromatography, DEAE-Cellulose; D003600:Cytosol / Q000201:enzymology; D004592:Electrophoresis, Starch Gel; D006041:Goats / Q000469:parasitology; D015801:Helminth Proteins / Q000302:isolation & purification*; D007527:Isoenzymes / Q000302:isolation & purification*; D008928:Mitochondria / Q000201:enzymology; D011725:Pyridines / Q000494:pharmacology; D013482:Superoxide Dismutase / Q000037:antagonists & inhibitors / Q000302:isolation & purification*; D014258:Trichuris / Q000201:enzymology*
D001562:Benzimidazoles; D015801:Helminth Proteins; D007527:Isoenzymes; D011725:Pyridines; D013482:Superoxide Dismutase