Sequence of human galanin and its inhibition of glucose-stimulated insulin secretion from RIN cells.
Human progalanin cDNA was cloned with polymerase chain reaction techniques. The cDNA sequence predicts that the human form of galanin has a substitution of the glycine residue found at position 30 in other species and thus is likely to retain this residue during posttranslational processing and not be amidated at the COOH terminus. Furthermore, the cDNA sequence predicts three additional amino acid substitutions compared with known galanins. Human galanin was synthesized, and its bioactivity was compared with porcine and rat galanin based on inhibition of insulin release from a glucose-responsive rat insulinoma (RIN) cell line. Human galanin inhibited glucose-stimulated insulin secretion in a dose-dependent manner in RIN cells. Human, porcine, and rat galanin exhibited similar activity with ED50 less than 1 nM.
McKnight
G L
GL
ZymoGenetics, Inc., Seattle, WA 98105.
Karlsen
A E
AE
Kowalyk
S
S
Mathewes
S L
SL
Sheppard
P O
PO
O'Hara
P J
PJ
Taborsky
G J
GJ
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002460:Cell Line; D003001:Cloning, Molecular / Q000379:methods; D019004:Galanin; D005947:Glucose / Q000494:pharmacology*; D006801:Humans; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D007340:Insulinoma; D007700:Kinetics; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D010190:Pancreatic Neoplasms; D010455:Peptides / Q000235:genetics* / Q000494:pharmacology*; D011061:Poly A / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods; D011498:Protein Precursors / Q000235:genetics*; D012313:RNA / Q000235:genetics; D012333:RNA, Messenger; D051381:Rats; D012689:Sequence Homology, Nucleic Acid; D013552:Swine
D007328:Insulin; D009838:Oligodeoxyribonucleotides; D010455:Peptides; D011498:Protein Precursors; D012333:RNA, Messenger; D011061:Poly A; D012313:RNA; D019004:Galanin; D005947:Glucose