Calcium channel blocking agents and potassium-stimulated release of glutamate from cerebellar slices.
The effects of calcium channel blockers and tetrodotoxin on the potassium-stimulated release of endogenous glutamate from rat cerebellar slices was assessed. Verapamil (10 microM), omega-conotoxin (1 and 10 microM) and cobalt (2 mM) all significantly decreased release. Amiloride (100 microM) and tetrodotoxin (0.5 and 1 microM) had no effect. The results suggest a role for N-type voltage-operated calcium channels in the potassium-stimulated release of glutamate.
Department of Pharmacology and Therapeutics, University of Wales College of Medicine, Heath Park, Cardiff, UK.
B G
BG
Dickie
J A
JA
Davies
D000818:Animals; D002118:Calcium / Q000502:physiology; D002121:Calcium Channel Blockers / Q000494:pharmacology*; D002531:Cerebellum / Q000187:drug effects* / Q000378:metabolism; D005260:Female; D005971:Glutamates / Q000378:metabolism*; D018698:Glutamic Acid; D066298:In Vitro Techniques; D011188:Potassium / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
D002121:Calcium Channel Blockers; D005971:Glutamates; D018698:Glutamic Acid; D011188:Potassium; D002118:Calcium