Generation of a subtype-specific neutralization epitope in foot-and-mouth disease virus of a different subtype.
An epitope involved in neutralization of foot-and-mouth disease virus (FMDV) of subtype C3 was generated by a single amino acid replacement in VP1 of FMDV of subtype C1. The replacement [Ser (139)----Ile, in the immunodominant site A] was consistently found in those FMDV C1 Santa Pau-Sp/70 mutants resistant to neutralization by monoclonal antibody (MAb) SD6 (specific for most C1 viruses) that acquired the capacity to be neutralized by MAb 7AB5 (specific for C3 viruses).
Centro de Biología Molecular, Universidad Autónoma de Madrid, Spain.
J
J
Hernández
M A
MA
Martínez
E
E
Rocha
E
E
Domingo
M G
MG
Mateu
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal; D000956:Antigens, Viral / Q000737:chemistry / Q000276:immunology*; D005537:Aphthovirus / Q000737:chemistry / Q000276:immunology*; D000939:Epitopes / Q000737:chemistry / Q000276:immunology*; D008969:Molecular Sequence Data; D009154:Mutation; D009500:Neutralization Tests; D010948:Viral Plaque Assay
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D000939:Epitopes