A Drosophila metallothionein promoter is inducible in mosquito cells.
Expression from a Drosophila metallothionein promoter (Mtn) was investigated in mosquito cells. Recombinant plasmids carrying a transcription unit comprised of the Escherichia coli beta-galactosidase gene (lacZ) fused to the metallothionein promoter were stably introduced into Aedes albopictus C6/36 cells. A low copy transformant containing approximately 60 copies of plasmid per cell, and a high copy transformant (1-2 x 10(4) copies/cell) were characterized. The expression of beta-galactosidase from the metallothionein promoter could be induced and controlled in this heterologous system, even when the copy number of introduced plasmid was several thousand.
Kovach
M J
MJ
Department of Microbiology, Colorado State University, Fort Collins 80526.
Carlson
J O
JO
Beaty
B J
BJ
D000330:Aedes / Q000737:chemistry / Q000235:genetics*; D000818:Animals; D002460:Cell Line; D003001:Cloning, Molecular; D004331:Drosophila melanogaster / Q000235:genetics*; D005786:Gene Expression Regulation; D005822:Genetic Vectors; D008668:Metallothionein / Q000235:genetics* / Q000302:isolation & purification; D011401:Promoter Regions, Genetic; D001616:beta-Galactosidase / Q000235:genetics
D008668:Metallothionein; D001616:beta-Galactosidase