Continuous chitosan hydrolyzate production by immobilized chitosanolytic enzyme from Enterobacter sp. G-1.
Chitosanolytic enzymes from Enterobacter sp. G-1 were immobilized on various carriers to continuously hydrolyze chitosan. Four different carriers were tested: FE-3901 (strong basic anion exchange resin, ionic binding), glutaraldehyde-treated FE-4612 (weak basic anion exchange resin, cross-linking), Chitopearl (chitosan beads), and alginate calcium. Glutaraldehyde-treated FE-4612 and Chitopearl immobilized more protein than the others. The enzyme immobilized on FE-3901 had the greatest activity. The activity of enzyme immobilized on FE-3901 decreased rapidly when exposed to a continuous flow of 1% chitosan. The enzyme immobilized with Chitopearl retained more than 50% of its original activity after 17 days, and the activity was fully restored by re-immobilization.
Yamasaki
Y
Y
Applied Biological Sciences, Faculty of Agriculture, Shimane University, Matsue, Japan.
Fukumoto
I
I
Kumagai
N
N
Ohta
Y
Y
Nakagawa
T
T
Kawamukai
M
M
Matsuda
H
H
D000837:Anion Exchange Resins; D001426:Bacterial Proteins / Q000378:metabolism*; D002686:Chitin / Q000031:analogs & derivatives* / Q000378:metabolism; D048271:Chitosan; D004754:Enterobacter / Q000201:enzymology*; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000378:metabolism*; D006026:Glycoside Hydrolases / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007526:Isoelectric Point; D007700:Kinetics; D011506:Proteins / Q000378:metabolism
D000837:Anion Exchange Resins; D001426:Bacterial Proteins; D004800:Enzymes, Immobilized; D011506:Proteins; D002686:Chitin; D048271:Chitosan; D006026:Glycoside Hydrolases; C019868:chitosanase