Antigen-specific and polyclonal CD4+ lamina propria T-cell lines: phenotypic and functional characterization.
Surface phenotype and function of lamina propria CD4+ T cells have been evaluated. In addition, long-term, antigen-specific and polyclonal lamina propria CD4+ T-cell lines have been generated and characterized. Lamina propria CD4+ T cells represent approximately 30% of lamina propria lymphocytes and are responsive to a variety of T-cell mitogens, including anti-CD3, concanavalin A, phytohaemagglutinin and pokeweed mitogen. In each case, however, lamina propria T cells are less responsive to these mitogens than spleen T cells. Freshly isolated lamina propria T cells produce substantial amounts of interleukin-2 (IL-2), interleukin-4 (IL-4), gamma interferon and to a lesser extent interleukin-5 (IL-5). Antigen-specific lamina propria CD4+ T-cell lines were generated by orally immunizing animals with antigen (KLH) in conjunction with cholera toxin as an oral adjuvant. Polyclonal lamina propria CD4+ T-cell lines were generated from unimmunized animals using anti-CD3 as a polyclonal stimulus. Both antigen-specific and polyclonal CD4+ T-cell lines were Thy-1+, alpha beta TCR+ and CD8-. The antigen-specific CD4+ T-cell line when stimulated by anti-CD3 and PMA produces predominantly IL-2, IL-4 and gamma interferon, with very little IL-5. In contrast, the polyclonal CD4+ T-cell line when similarly stimulated produces predominantly IL-4 and IL-5, with very little IL-2 and no detectable gamma interferon. In summary, lamina propria CD4+ T cells have been evaluated and in vitro conditions have been determined for successful generation of lamina propria CD4+ T-cell lines.
Mucosal Immunity Section, NIAID, NIH, Bethesda, MD.
G R
GR
Harriman
E
E
Hörnqvist
N Y
NY
Lycke
D000818:Animals; D000954:Antigens, Surface / Q000032:analysis; D015704:CD4 Antigens / Q000032:analysis*; D002455:Cell Division / Q000276:immunology; D002460:Cell Line; D002478:Cells, Cultured; D000939:Epitopes / Q000276:immunology; D007413:Intestinal Mucosa / Q000276:immunology*; D007421:Intestine, Small / Q000276:immunology; D008222:Lymphokines / Q000096:biosynthesis; D051379:Mice; D008810:Mice, Inbred C57BL; D013601:T-Lymphocytes / Q000276:immunology*
D000954:Antigens, Surface; D015704:CD4 Antigens; D000939:Epitopes; D008222:Lymphokines