Immunohistochemical determination of P-glycoprotein in a rat mammary tumour treated with tamoxifen.
The effect of treatment with the antiestrogen Tamoxifen on the distribution and localization of P-glycoprotein was determined by immunohistochemistry in a rat mammary tumour model, the R3230AC. Both in the untreated and the treated tumours, P-glycoprotein is unevenly expressed with numerous negative tumour cells and located predominantly in the cytoplasmic membranes. Administration of Tamoxifen significantly lowers P-glycoprotein content of the tumour studied.
Kellen
J A
JA
Sunnybrook Health Science Centre, University of Toronto, Ontario, Canada.
Wong
A C
AC
Mirakian
A
A
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D000230:Adenocarcinoma / Q000737:chemistry* / Q000188:drug therapy; D000818:Animals; D002352:Carrier Proteins / Q000032:analysis*; D004351:Drug Resistance; D005260:Female; D007124:Immunoenzyme Techniques; D008325:Mammary Neoplasms, Experimental / Q000737:chemistry* / Q000188:drug therapy; D008562:Membrane Glycoproteins / Q000032:analysis*; D009363:Neoplasm Proteins / Q000032:analysis*; D009376:Neoplasms, Hormone-Dependent / Q000737:chemistry* / Q000188:drug therapy; D051381:Rats; D011916:Rats, Inbred F344; D013629:Tamoxifen / Q000494:pharmacology* / Q000627:therapeutic use
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D002352:Carrier Proteins; D008562:Membrane Glycoproteins; D009363:Neoplasm Proteins; D013629:Tamoxifen