Cell proliferation in rat colon measured with bromodeoxyuridine, proliferating cell nuclear antigen, and [3H]thymidine.
Epithelial cell proliferation was studied in the normal colonic mucosa of 5-week-old Sprague-Dawley rats, comparing [3H]thymidine incorporation (group 1) with two newer proliferation markers, bromodeoxyuridine (group 2) and proliferating cell nuclear antigen (group 3). Microautoradiography (group 1) or immunoperoxidase assays (groups 2 and 3) were carried out. Cells were counted for positive reaction and position along 50 colonic crypt columns/animal. No significant differences were found in number or distribution of labeled epithelial cells in proliferative compartments in crypt columns of normal colonic mucosa; labeled cells were mainly in the lower 60% of colonic crypts. Thus, in this model, bromodeoxyuridine and proliferating cell nuclear antigens were comparable to [3H]thymidine as reliable markers of proliferating epithelial cells in rat colon.
Irving Weinstein Laboratory for Gastrointestinal Cancer Prevention, Memorial Sloan-Kettering Cancer Center, New York, New York 10021.
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Richter
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Richter
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Yang
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Lipkin
D000818:Animals; D001324:Autoantigens; D015415:Biomarkers; D001973:Bromodeoxyuridine; D002452:Cell Count; D002455:Cell Division; D004847:Epithelial Cells; D005069:Evaluation Studies as Topic; D005260:Female; D005404:Fixatives; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000166:cytology*; D009687:Nuclear Proteins; D018809:Proliferating Cell Nuclear Antigen; D051381:Rats; D017207:Rats, Sprague-Dawley; D013936:Thymidine; D014316:Tritium
D001324:Autoantigens; D015415:Biomarkers; D005404:Fixatives; D009687:Nuclear Proteins; D018809:Proliferating Cell Nuclear Antigen; D014316:Tritium; D001973:Bromodeoxyuridine; D013936:Thymidine