Mathematical modelling and simulation of aqueous two-phase continuous protein extraction.
A mathematical model has been developed to describe the continuous, steady-state operation of an aqueous two-phase system for protein extraction. The model is based on steady-state mass balances of the main components and phase equilibrium data. Experimental data on the separation of thaumatin from contaminant proteins of an homogenate of E. coli in a PEG4000/Phosphate system was used. The data shows the effect of the presence and absence of NaCl which was used to carry out the extraction of thaumatin into the PEG phase and back into the PO4(-3) phase. Simulation results showing the sensitivity to key process parameters, and the effect of process variables on performance are presented and discussed. The model can be used to predict performance and thus 'robustness' of process conditions as well as predict protein recovery yield and purity. This model can also be used to implement a suitable control strategy to maintain process stability.
Mistry
S L
SL
University of Reading, Biochemical Engineering Laboratory, England.
Asenjo
J A
JA
Zaror
C A
CA
D001426:Bacterial Proteins / Q000302:isolation & purification*; D004340:Drug Contamination; D004926:Escherichia coli; D008433:Mathematics; D008722:Methods; D008962:Models, Theoretical; D010710:Phosphates; D010940:Plant Proteins / Q000302:isolation & purification*; D011092:Polyethylene Glycols; D011506:Proteins / Q000302:isolation & purification*; D013549:Sweetening Agents
D001426:Bacterial Proteins; D010710:Phosphates; D010940:Plant Proteins; D011506:Proteins; D013549:Sweetening Agents; D011092:Polyethylene Glycols; C003427:thaumatin protein, plant