Species differentiation of mycoplasmas by EF-Tu specific monoclonal antibodies.
Ten mouse hybridoma cell lines producing IgG monoclonal antibodies to mycoplasmal elongation factor Tu (EF-Tu) were established. These mAbs showed different degrees of cross-reactivity between mollicutes and even other bacteria. This finding, indicating protein structure diversities of pan bacterial EF-Tu should permit species differentiation of mycoplasmas by epitope pattern analysis of a single protein. Epitope patterns of 23 mollicute type strains and of 40 M. hominis isolates were determined by ELISA. All M. hominis patterns were found to be closely related whereas intrageneric patterns differed in a species specific manner.
Institute for Medical Microbiology and Virology, Heinrich-Heine-University, Düsseldorf, Germany.
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Kamla
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Henrich
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Hadding
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D000942:Antigens, Bacterial / Q000032:analysis; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000032:analysis*; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009174:Mycoplasma / Q000145:classification / Q000276:immunology*; D010444:Peptide Elongation Factor Tu / Q000276:immunology*; D013045:Species Specificity
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D000939:Epitopes; D010444:Peptide Elongation Factor Tu