Glutamine and glucose metabolism in bovine blood lymphocytes.
1. Glutamine and glucose metabolism was studied in bovine blood lymphocytes incubated at 37 degrees C in the presence of Krebs-Ringer bicarbonate buffer (pH 7.4) containing 1 mM [U-14C]glutamine and 5 mM [U-14C]glucose, respectively. 2. The major metabolic products from glutamine were ammonia, glutamate, and to a lesser extent, aspartate and CO2. Glucose was metabolized mainly to lactate and, to a lesser extent, pyruvate and CO2. These findings indicate incomplete oxidation of glutamine and glucose carbons in bovine blood lymphocytes. 3. Glucose provided three-fold greater amounts of energy to bovine blood lymphocytes than did glutamine on the basis of their measured end-products. Glycolysis accounted for 50% of glucose-derived ATP production. 4. Our findings suggest similar metabolic patterns of glutamine and glucose in lymphocytes between ruminants and non-ruminant species (e.g. rats). However, in contrast to rat peripheral lymphocytes, glucose, rather than glutamine, was a major energy substrate for bovine blood lymphocytes.
Wu
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Department of Animal Science, Texas A&M University, College Station 77843-2471.
Greene
L W
LW
D000255:Adenosine Triphosphate / Q000096:biosynthesis; D000641:Ammonia / Q000097:blood; D000818:Animals; D001224:Aspartic Acid / Q000097:blood; D001786:Blood Glucose / Q000378:metabolism*; D002417:Cattle / Q000097:blood*; D005971:Glutamates / Q000097:blood; D018698:Glutamic Acid; D005973:Glutamine / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D007773:Lactates / Q000097:blood; D019344:Lactic Acid; D008214:Lymphocytes / Q000378:metabolism*; D008297:Male; D011773:Pyruvates / Q000097:blood; D019289:Pyruvic Acid; D051381:Rats
D001786:Blood Glucose; D005971:Glutamates; D007773:Lactates; D011773:Pyruvates; D005973:Glutamine; D001224:Aspartic Acid; D019344:Lactic Acid; D018698:Glutamic Acid; D000641:Ammonia; D019289:Pyruvic Acid; D000255:Adenosine Triphosphate