Piperine-mediated changes in the permeability of rat intestinal epithelial cells. The status of gamma-glutamyl transpeptidase activity, uptake of amino acids and lipid peroxidation.
The effect of piperine (1-[5-(1,3-benzodioxol-5-yl)-1-oxo-2,4-pentadienyl]piperidin e), (from Piper nigrum) on the absorptive function of the intestine was studied. In vitro experiments showed that piperine (25-100 microM) significantly stimulated gamma-glutamyl transpeptidase (gamma-GT, EC 2.3.2.2.) activity, enhanced the uptake of radiolabelled L-leucine, L-isoleucine and L-valine, and increased lipid peroxidation in freshly isolated epithelial cells of rat jejunum. The kinetic behaviour of gamma-GT towards substrate and acceptor altered in the presence of piperine. In the presence of benzyl alcohol, an enhanced gamma-GT activity due to piperine was maintained. These results suggested that piperine may interact with the lipid environment to produce effects which lead to increased permeability of the intestinal cells.
Regional Research Laboratory (CSIR), Jammu-Tawi, India.
R K
RK
Johri
N
N
Thusu
A
A
Khajuria
U
U
Zutshi
D000470:Alkaloids; D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D052117:Benzodioxoles; D019905:Benzyl Alcohol; D001592:Benzyl Alcohols; D002463:Cell Membrane Permeability / Q000187:drug effects*; D004789:Enzyme Activation / Q000187:drug effects; D004848:Epithelium / Q000187:drug effects; D007422:Intestines / Q000187:drug effects*; D007583:Jejunum / Q000187:drug effects; D007700:Kinetics; D015227:Lipid Peroxidation / Q000187:drug effects*; D010880:Piperidines / Q000494:pharmacology*; D011092:Polyethylene Glycols; D053284:Polyunsaturated Alkamides; D051381:Rats; D005723:gamma-Glutamyltransferase / Q000378:metabolism*
D000470:Alkaloids; D000596:Amino Acids; D052117:Benzodioxoles; D001592:Benzyl Alcohols; D010880:Piperidines; D053284:Polyunsaturated Alkamides; D011092:Polyethylene Glycols; D005723:gamma-Glutamyltransferase; D019905:Benzyl Alcohol; C008922:piperine