A double-label analysis demonstrating the non-coexistence of tyrosine hydroxylase-like and GABA-like immunoreactivities in amacrine cells of the larval tiger salamander retina.
Previous studies have localized tyrosine hydroxylase, the rate-limiting enzyme for the production of dopamine, and gamma-aminobutyric acid (GABA) to amacrine cell populations in the larval tiger salamander retina. Double-label immunocytochemistry was used to examine if tyrosine hydroxylase-like and GABA-like immunoreactivities colocalize in tiger salamander amacrine cells. A total of 2,162 tyrosine hydroxylase-like immunoreactive amacrine cells were observed in double-labelled sections. None of these cells were observed to express GABA-like immunoreactivity. Therefore, the present study demonstrates that dopamine and GABA are localized to distinct neuronal populations in the larval tiger salamander retina.
Watt
C B
CB
Alice R. McPherson Laboratory of Retina Research, Baylor College of Medicine, The Woodlands, TX 77381.
Florack
V J
VJ
D000818:Animals; D005455:Fluorescent Antibody Technique; D007150:Immunohistochemistry; D007814:Larva; D012160:Retina / Q000166:cytology*; D014446:Tyrosine 3-Monooxygenase / Q000032:analysis*; D014562:Caudata; D005680:gamma-Aminobutyric Acid / Q000032:analysis*
D005680:gamma-Aminobutyric Acid; D014446:Tyrosine 3-Monooxygenase