Exon skipping in human beta-casein.
Earlier amino acid alignments of mature beta-caseins showed that the human protein was shifted in alignment relative to other species, with amino acid deletions in the N-terminal region and others inserted in the C-terminal region. Our alignment, based on cDNA sequences and their translation products, has shown that the amino acid deletions correspond exactly to exon 3 in the other species. Cloning and sequencing of a segment of the human beta-casein gene between exons 2 and 4 revealed the presence of an intact exon 3 sequence in the gene. An interruption of the polypyrimidine tract adjacent to the 5' end of exon 3 sequence may account for the omission of the exon from human beta-casein mRNA.
Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder 80309.
R S
RS
Menon
Y F
YF
Chang
K F
KF
Jeffers
R G
RG
Ham
D000595:Amino Acid Sequence; D001483:Base Sequence; D002364:Caseins / Q000235:genetics*; D003001:Cloning, Molecular; D005091:Exons; D006801:Humans; D008969:Molecular Sequence Data; D012313:RNA; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid
D002364:Caseins; D012313:RNA