Antibody assisted protein refolding.
In this study, we provide an initial demonstration of the use of monoclonal antibodies (MAbs) to enhance the yield of native protein during protein refolding. The presence of an anti-native MAb was found to enhance the refolding of reduced S-Protein (a fragment of Ribonuclease A) by as much as 360 percent over controls. This increase in recovered enzymatic activity was directly related to the MAb concentration and was saturable with excess antibody, suggesting that the antibodies are assisting through direct interaction at specific epitopes.
Carlson
J D
JD
Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge 02139.
Yarmush
M L
ML
D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G / Q000145:classification; D007700:Kinetics; D046911:Macromolecular Substances; D008970:Molecular Weight; D010084:Oxidation-Reduction; D010446:Peptide Fragments / Q000737:chemistry* / Q000276:immunology; D011487:Protein Conformation; D011489:Protein Denaturation; D011506:Proteins / Q000737:chemistry* / Q000276:immunology; D012259:Ribonuclease, Pancreatic / Q000737:chemistry* / Q000276:immunology
D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D046911:Macromolecular Substances; D010446:Peptide Fragments; D011506:Proteins; C074799:ribonuclease A (21-124); D012259:Ribonuclease, Pancreatic