Intrinsic radiosensitivity and PLD repair in osteosarcoma cell lines.
The response to radiation of seven osteosarcoma cell lines was analysed by in vitro colony-forming assay and compared with that of eight human fibroblast strains. The values of D0, the surviving fraction after 2 Gy (S2Gy), and the mean inactivation dose (D) of osteosarcoma cells in log-phase culture were significantly higher than those of fibroblast strains (p less than 0.01). PLD (potentially lethal damage) repair of osteosarcoma cells evaluated in the plateau phase of growth showed great variation for enhancement of survival, although all of the values were maximized within 12 h after irradiation. In the osteosarcoma, intrinsic radiosensitivity in vitro reflected the clinical response to radiation. However, the capacity for PLD repair might not be a good indicator for predicting the results of radiation therapy.
Department of Orthopaedic Surgery, Faculty of Medicine, Kyoto University, Japan.
M
M
Sugimoto
J
J
Toguchida
Y
Y
Kotoura
T
T
Yamamuro
H
H
Utsumi
D002470:Cell Survival / Q000528:radiation effects; D004260:DNA Repair; D005347:Fibroblasts / Q000528:radiation effects; D006801:Humans; D012516:Osteosarcoma / Q000532:radiotherapy*; D011829:Radiation Dosage; D011836:Radiation Tolerance; D013997:Time Factors; D014407:Tumor Cells, Cultured