The human blood fluke Schistosoma mansoni synthesizes glycoproteins containing the Lewis X antigen.
Infection of vertebrates with the parasitic blood fluke Schistosoma mansoni induces a variety of host immune responses, which are directed against both protein and carbohydrate antigens. In this report, we describe our studies on the structures of antigenic oligosaccharides derived from glycoproteins synthesized by S. mansoni. Immobilized antibodies derived from the sera of infected hamsters and mice bind to a family of high molecular weight Asn-linked oligosaccharides in glycoproteins from the adult parasite. Structural analysis of the major antigenic oligosaccharides revealed that they have high amounts of fucose-linked alpha 1,3 to N-acetylglucosamine residues within the linear repeating disaccharide (3Gal beta 1-4GlcNAc beta 1)n, a poly-N-acetyllactosamine sequence containing the Lewis X antigenic blood group. The remarkable ability of S. mansoni to synthesize these vertebrate-type oligosaccharides may have implications in both the mechanisms of host-parasite interactions and on the development of vaccines to prevent this disease in humans.
University of Georgia, Department of Biochemistry, Athens 30602.
J
J
Srivatsan
D F
DF
Smith
R D
RD
Cummings
D000818:Animals; D000947:Antigens, Helminth / Q000096:biosynthesis* / Q000276:immunology; D015153:Blotting, Western; D002240:Carbohydrate Sequence; D002846:Chromatography, Affinity; D006224:Cricetinae; D004591:Electrophoresis, Polyacrylamide Gel; D005690:Galactose / Q000737:chemistry; D006023:Glycoproteins / Q000096:biosynthesis* / Q000737:chemistry / Q000276:immunology; D006026:Glycoside Hydrolases; D016256:Lewis X Antigen / Q000096:biosynthesis*; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D012550:Schistosoma mansoni / Q000378:metabolism*; D001616:beta-Galactosidase / Q000737:chemistry
D000947:Antigens, Helminth; D006023:Glycoproteins; D016256:Lewis X Antigen; D006026:Glycoside Hydrolases; C026093:keratan-sulfate endo-1,4-beta-galactosidase; D001616:beta-Galactosidase; D005690:Galactose