Engineering enzymes for non-aqueous solvents.
Enzymes may be redesigned to permit catalysis in non-aqueous solvents by engineering their amino acid sequences, thereby altering their physical and chemical properties to suit the new solvent environment. The interactions that contribute to protein stability in non-aqueous solvents are discussed in the context of attempting to identify possible approaches to constructing enzymes which exhibit enhanced stability in non-aqueous media. These approaches are illustrated by several examples where protein engineering has resulted in enzymes that are better suited for catalysis in organic solvents.
Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena 91125.
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Arnold
D000595:Amino Acid Sequence; D002384:Catalysis; D004795:Enzyme Stability; D004798:Enzymes / Q000737:chemistry / Q000235:genetics*; D009154:Mutation; D011487:Protein Conformation; D015202:Protein Engineering; D012997:Solvents; D013381:Subtilisins / Q000235:genetics
D004798:Enzymes; D012997:Solvents; D013381:Subtilisins