Enhanced antibody titers to an oxidized DNA base in inflammatory and neoplastic diseases.
Reactive oxygen species (ROS) produced by phagocytic cells induce oxidative stress during chronic inflammation. ROS play a role in the pathogenesis of a broad range of diseases including autoimmune, cardiac and neoplastic abnormalities. We found that sera of patients with a variety of inflammatory dermatoses contain elevated levels of antibodies (Ab) binding to an oxidized DNA base derivative, 5-hydroxymethyl-2'deoxyuridine (HMdU) coupled to bovine serum albumin, as determined by the enzyme-linked immunosorbent assay. Patients with immune complex diseases and a history of neoplasm elaborated the highest titers of anti-HMdU Ab. Titers from sera of psoriatic subjects were lower than from the aforementioned groups but were still significantly elevated (p < 0.001) above those of healthy controls. Treatment of inflammatory dermatoses with systemic antiinflammatory and cytotoxic drugs significantly lowered the titers [p < 0.005 (immune complex) or p < 0.001 (psoriasis and neoplastic) diseases], suggesting that this assay may be of value in monitoring the response to therapy in these diseases.
Department of Environmental Medicine, New York University Medical Center, NY 10016-6451.
K
K
Frenkel
D
D
Khasak
J
J
Karkoszka
J
J
Shupack
M
M
Stiller
D000328:Adult; D000368:Aged; D000906:Antibodies / Q000032:analysis*; D000936:Antigen-Antibody Complex; D004247:DNA / Q000378:metabolism; D003872:Dermatitis / Q000276:immunology*; D005260:Female; D006801:Humans; D007154:Immune System Diseases / Q000276:immunology; D008297:Male; D008875:Middle Aged; D009369:Neoplasms / Q000276:immunology*; D011565:Psoriasis / Q000276:immunology; D017382:Reactive Oxygen Species / Q000378:metabolism; D013936:Thymidine / Q000031:analogs & derivatives* / Q000276:immunology; D014647:Varicose Ulcer / Q000276:immunology
D000906:Antibodies; D000936:Antigen-Antibody Complex; D017382:Reactive Oxygen Species; C018080:5-hydroxymethyl-2'-deoxyuridine; D004247:DNA; D013936:Thymidine