Engineering enzymes for non-aqueous solvents.
Enzymes may be redesigned to permit catalysis in non-aqueous solvents by engineering their amino acid sequences, thereby altering their physical and chemical properties to suit the new solvent environment. The interactions that contribute to protein stability in non-aqueous solvents are discussed in the context of attempting to identify possible approaches to constructing enzymes which exhibit enhanced stability in non-aqueous media. These approaches are illustrated by several examples where protein engineering has resulted in enzymes that are better suited for catalysis in organic solvents.
Arnold
F H
FH
Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena 91125.
D000595:Amino Acid Sequence; D002384:Catalysis; D004795:Enzyme Stability; D004798:Enzymes / Q000737:chemistry / Q000235:genetics*; D009154:Mutation; D011487:Protein Conformation; D015202:Protein Engineering; D012997:Solvents; D013381:Subtilisins / Q000235:genetics
D004798:Enzymes; D012997:Solvents; D013381:Subtilisins