Antisense RNA directed against the major capsid protein of Lactococcus lactis subsp. cremoris bacteriophage 4-1 confers partial resistance to the host.
Antisense RNA targeted against the major capsid protein (MCP) of Lactococcus lactis subsp. cremoris bacteriophage F4-1 reduced bacteriophage replication by up to 50%. The region containing the mcp gene was oriented to transcribe the antisense strand using a L. lactis subsp. cremoris Wg2 promoter. The size of the mcp insert transcribed affected the level of bacteriophage inhibition and the greatest level of inhibition was achieved using a 301-bp fragment from the 5' end of the mcp. Antisense mcp RNA constructs were stable and did not alter the endogenous plasmid profile in the host, L. lactis subsp. cremoris F4-1. There were, however, some adverse effects on the host during the stationary phase as exhibited by a decline in cell density.
Chung
D K
DK
Department of Food Science, Cornell University, Ithaca, NY 14853.
Chung
S K
SK
Batt
C A
CA
D001435:Bacteriophages / Q000187:drug effects*; D001483:Base Sequence; D002213:Capsid / Q000096:biosynthesis*; D002611:Cheese / Q000382:microbiology; D005524:Food Technology; D005822:Genetic Vectors; D013294:Lactococcus lactis / Q000254:growth & development*; D008969:Molecular Sequence Data; D010957:Plasmids; D016372:RNA, Antisense / Q000494:pharmacology*; D014779:Virus Replication / Q000187:drug effects*
D016372:RNA, Antisense