Continuous counter-current two-phase aqueous extraction.
Continuous counter-current column operation provides operating convenience for contacting two-phase aqueous partitioning systems for protein extraction. We discuss in detail the important parameters for designing spray, packed, plate and York-Scheibel columns for protein recovery using both polymer-polymer and polymer-salt two-phase aqueous systems. We compare the various contractors for their operating and extraction efficiency. The work also provides a step-by-step design procedure and specific recommendations for future data needs.
Department of Chemical Technology, University of Bombay, India.
J B
JB
Joshi
S B
SB
Sawant
K S
KS
Rao
T A
TA
Patil
K M
KM
Rostami
S K
SK
Sikdar
D008722:Methods; D011506:Proteins / Q000302:isolation & purification*; D012995:Solubility; D009626:Terminology as Topic; D014867:Water / Q000737:chemistry
D011506:Proteins; D014867:Water