Use of a dispersed repetitive DNA element to distinguish clinical isolates of Cryptococcus neoformans.
We isolated a recombinant phage from a Cryptococcus neoformans genomic library that contains a member of a dispersed family of repetitive DNA elements. This clone, CNRE-1, hybridized to at least seven chromosomes in C. neoformans on the basis of pulsed-field gel analysis. Hybridization of CNRE-1 to restriction digests of genomic DNA confirmed that there are multiple copies of this element and that restriction fragment length polymorphisms are present in strains from different serotypes of C. neoformans. The utility of this probe as an epidemiologic marker was determined by testing cryptococcal isolates from a single hospital. Five isolates from four patients were closely related to a serotype A reference strain, whereas five other isolates from four additional patients exhibited distinct patterns. In two patients, the isolates obtained during recurrent cryptococcal infections were identical to the original isolates.
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Department of Pathology, School of Medicine, State University of New York, Stony Brook 11794-8691.
Spitzer
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D000163:Acquired Immunodeficiency Syndrome / Q000382:microbiology; D003455:Cryptococcus neoformans / Q000235:genetics / Q000302:isolation & purification*; D004271:DNA, Fungal / Q000032:analysis*; D006801:Humans; D012150:Polymorphism, Restriction Fragment Length; D012091:Repetitive Sequences, Nucleic Acid
D004271:DNA, Fungal