An improved amperometric immunosensor for the detection and enumeration of protein A-bearing Staphylococcus aureus.
An amperometric immunosensor specific to the protein A of Staphylococcus aureus, was developed using the direct electrochemical detection of phenol produced by alkaline phosphatase from phenylphosphate. The immunosensor could detect protein A at 0.01 ng/ml and could reliably detect and quantify pure cultures of protein A-bearing Staph. aureus above 10(3) cfu/ml. A similar sensitivity of detection was obtained with samples of beef and milk.
Department of Microbiology, AFRC Institute of Food Research, Shinfield, Reading, UK.
B
B
Mirhabibollahi
J L
JL
Brooks
R G
RG
Kroll
D000818:Animals; D015374:Biosensing Techniques; D015169:Colony Count, Microbial; D004553:Electric Conductivity; D005516:Food Microbiology; D007159:Immunologic Tests; D008460:Meat; D008892:Milk / Q000382:microbiology; D019800:Phenol; D010636:Phenols / Q000032:analysis* / Q000378:metabolism; D011237:Predictive Value of Tests; D013205:Staphylococcal Protein A / Q000096:biosynthesis*; D013211:Staphylococcus aureus / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism
D010636:Phenols; D013205:Staphylococcal Protein A; D019800:Phenol