Nucleotide and deduced amino acid sequence of bovine adrenal medulla chromogranin B (secretogranin I).
1. A novel 1745-dalton pyroglutamyl peptide (BAM-1745)6 was recently isolated and characterized from bovine adrenal medulla chromaffin granules. Its amino acid sequence was found to be 93% identical to residues 580-593 of human chromogranin B (secretogranin I). 2. Based on this sequence a degenerate oligonucleotide probe was synthesized and used to identify a 2.4-kb bovine adrenal medulla chromogranin B cDNA. 3. The deduced polypeptide is 647 amino acids long and begins with a putative signal sequence of 20 residues as in the human, rat, and mouse proteins. Also conserved in the bovine protein is a tyrosine residue which may be sulfated, two N-terminal cysteines, and many paired basic amino acids which may serve as sites of posttranslational processing. The peptide BAM-1745 is flanked by paired basic amino acids and therefore is most likely a product of posttranslational processing. Bovine chromogranin B is 67, 58, and 58% identical to the human, rat, and mouse chromogranin B proteins, respectively. 4. The carboxyl terminus of bovine chromogranin B, including BAM-1745, was found to be the most conserved region of the polypeptide and may identify it as an important functional domain.
Grandy
D K
DK
Vollum Institute for Advanced Biomedical Research, Oregon Health Sciences University, Portland 97021.
Leduc
R
R
Makam
H
H
Flanagan
T
T
Diliberto
E J
EJ
Civelli
O
O
Viveros
O H
OH
D000313:Adrenal Medulla / Q000737:chemistry; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle / Q000235:genetics*; D053378:Chromogranin B; D002864:Chromogranins / Q000235:genetics* / Q000302:isolation & purification; D004247:DNA / Q000235:genetics; D006031:Glycosylation; D006801:Humans; D051379:Mice / Q000235:genetics; D008969:Molecular Sequence Data; D011061:Poly A / Q000235:genetics; D011499:Protein Processing, Post-Translational; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats / Q000235:genetics; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
C506134:CHGB protein, human; D053378:Chromogranin B; D002864:Chromogranins; D012333:RNA, Messenger; C506137:chromogranin B, mouse; C506140:chromogranin B, rat; D011061:Poly A; D004247:DNA