High speed automated DNA sequencing in ultrathin slab gels.
We have developed a high speed instrument for automated DNA sequence analysis. The apparatus employs laser excitation and a cooled CCD detector for the parallel detection of up to 18 sets of four fluorescently labeled DNA sequencing reactions during their electrophoretic separation in ultrathin (50-100 microns) denaturing polyacrylamide gels. Four hundred and fifty bases of sequence information is obtained from 100 ng of M13 template DNA in less than one hour, corresponding to an overall instrument throughput of over 8000 bases/hr.
Kostichka
A J
AJ
Department of Chemistry, University of Wisconsin-Madison 53706.
Marchbanks
M L
ML
Brumley
R L
RL
Drossman
H
H
Smith
L M
LM
D001322:Autoanalysis / Q000295:instrumentation / Q000379:methods; D001483:Base Sequence; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel / Q000295:instrumentation / Q000379:methods
D004247:DNA