On the value of giving a combination of drugs for the treatment of endotoxaemia in pigs.
OBJECTIVE
To see if treatment with a combination of drugs each directed at a different mediator was successful in preventing activation of those mediators in experimental endotoxic shock.


METHODS
Controlled study.


METHODS
40 juvenile pigs.


METHODS
33 animals received 0.01 mg/kg endotoxin infusion, the rest being given the same volume of saline; 10 of the 33 received no treatment. Of the remaining 23, 5 were given combination treatment with methylprednisolone, naloxone, ketanserin, promethazine, C1 esterase inhibitor, antithrombin III and aprotinin; 7 were given methylprednisolone only; 6 were given the three protease inhibitors (C1 esterase inhibitor, antithrombin III and aprotinin); and 5 were given naloxone, ketanserin and promethazine.


METHODS
Assessment of haemodynamic, proteolytic, and cellular effects of endotoxaemia.


RESULTS
Only the combination treatment totally blocked all the effects of the infusion of endotoxin.


CONCLUSIONS
As endotoxin affects several mediators, combination treatment is necessary to block all its deleterious effects in pigs.
Naess
F
F
Department of Surgery, Ullevål Hospital, University of Oslo, Norway.
Roeise
O
O
Stadaas
J O
JO
Aasen
A O
AO
D000818:Animals; D004338:Drug Combinations; D010450:Endopeptidases / Q000097:blood / Q000187:drug effects; D004731:Endotoxins / Q000097:blood; D004926:Escherichia coli; D006439:Hemodynamics / Q000187:drug effects; D012772:Shock, Septic / Q000097:blood / Q000188:drug therapy* / Q000401:mortality; D013552:Swine
D004338:Drug Combinations; D004731:Endotoxins; D010450:Endopeptidases