Purification of bacterial exotoxins. The case of botulinum, tetanus, anthrax, pertussis and cholera toxins.
Bacterial protein toxins and their fragments have been isolated and purified for various reasons, including the development of efficient vaccines and for methods of identification of bacterial agents causing disease. This activity continues today but a new area of bacterial protein toxin research has recently emerged. Since it was shown that toxin molecules comprise several types of biological activity within their structural domains, it was suggested to use these domains (and their combinations) as biochemical tools for developing novel agents for disease imaging and and/or relieving. In this way eukaryotic cell-receptor specific fusion toxins have been developed to prevent malignancy in human. While human clinical trials of these preparations have only recently begun, the preliminary clinical findings are promising. Also fusion proteins which combine independent immunodominant epitopes from different antigens have also been developed thus opening a way for the generation of new vaccines for both human and veterinary use. Receptor binding fragments of microbial toxins when combined with other molecules may be useful in delivering these molecules into the cell. In this way novel agents may be developed with a potential for inducing specific changes at the molecular level for the correction of metabolic disorders causing human and animal diseases. Bacterial protein toxins such as anthrax, botulinum, cholera, pertussis and tetanus for which considerable progress has been achieved in structure-function analysis are promising candidates for such research. Particularly exciting appears the idea of extending this research to the cells of the nervous system, exploiting the unique specificity of the botulinum or tetanus toxin fragments which may bring long desired methods for treatment of various disorders of the nervous system. Data on functional domains of these toxins as well as methods of purification of the whole toxins and their fragments are considered in this review as they form a base for their further structure-function analysis and engineering applications.
PHLS Centre for Applied Microbiology & Research, Porton Down, Salisbury, UK.
V A
VA
Pasechnik
C C
CC
Shone
P
P
Hambleton
D000818:Animals; D000942:Antigens, Bacterial; D001408:Bacillus anthracis / Q000378:metabolism; D001427:Bacterial Toxins / Q000302:isolation & purification*; D001905:Botulinum Toxins / Q000302:isolation & purification; D002772:Cholera Toxin / Q000302:isolation & purification; D002853:Chromatography, Liquid; D005098:Exotoxins / Q000302:isolation & purification*; D006801:Humans; D013744:Tetanus Toxin / Q000302:isolation & purification; D010566:Virulence Factors, Bordetella / Q000302:isolation & purification
D000942:Antigens, Bacterial; D001427:Bacterial Toxins; D005098:Exotoxins; D013744:Tetanus Toxin; D010566:Virulence Factors, Bordetella; C030325:anthrax toxin; D002772:Cholera Toxin; D001905:Botulinum Toxins