Study of humoral immune response in mammals immunized with Plasmodium falciparum antigenic preparations.
Six Plasmodium falciparum protein fractions, isolated under reducing conditions, were used to immunize mice, rabbits and the squirrel monkey Saimiri sciureus. Five or seven subcutaneous injections of each antigenic preparation, in conjunction with Freund's complete or incomplete adjuvants, were administered. This led to the development of specific antibodies detected by IFAT, ELISA or immunoblotting which inhibited merozoite reinvasion in in vitro P. falciparum cultures. This activity seems to be associated with rhoptry proteins contained in fractions Pf F2 and Pf F4.
Cubas
A B
AB
INSERM U 313, Faculté de Médicine Pitié-Salpêtrière, Paris, France.
Cunha
F
F
Nitcheu
I K
IK
Rolland
L
L
Ciceron
L
L
Carvalho
A B
AB
Furtado
A F
AF
Monjour
L
L
D000818:Animals; D000913:Antibodies, Protozoan / Q000096:biosynthesis* / Q000276:immunology; D000953:Antigens, Protozoan / Q000276:immunology*; D005260:Female; D007106:Immune Sera; D007117:Immunization, Secondary; D007074:Immunoglobulin G / Q000276:immunology; D007075:Immunoglobulin M / Q000276:immunology; D017780:Malaria Vaccines / Q000276:immunology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010963:Plasmodium falciparum / Q000254:growth & development / Q000276:immunology*; D015800:Protozoan Proteins / Q000276:immunology*; D011817:Rabbits; D012453:Saimiri; D014611:Vaccination
D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D007106:Immune Sera; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D017780:Malaria Vaccines; D015800:Protozoan Proteins