Cloning of the alpha-adducin gene from the Huntington's disease candidate region of chromosome 4 by exon amplification.
We have applied the technique of exon amplification to the isolation of genes from the chromosome 4p16.3 Huntington's disease (HD) candidate region. Exons recovered from cosmid Y24 identified cDNA clones corresponding to the alpha-subunit of adducin, a calmodulin-binding protein that is thought to promote assembly of spectrin-actin complexes in the formation of the membrane cytoskeleton, alpha-adducin is widely expressed and, at least in brain, is encoded by alternatively spliced mRNAs. The alpha-adducin gene maps immediately telomeric to D4S95, in a region likely to contain the HD defect, and must be scrutinized to establish whether it is the site of the HD mutation.
Molecular Neurogenetics Unit, Massachusetts General Hospital, Boston.
S A
SA
Taylor
R G
RG
Snell
A
A
Buckler
C
C
Ambrose
M
M
Duyao
D
D
Church
C S
CS
Lin
M
M
Altherr
G P
GP
Bates
N
N
Groot
D017398:Alternative Splicing / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001798:Blood Proteins / Q000235:genetics*; D001923:Brain Chemistry / Q000235:genetics; D002148:Calmodulin-Binding Proteins / Q000032:analysis / Q000737:chemistry / Q000235:genetics*; D002894:Chromosomes, Human, Pair 4; D003001:Cloning, Molecular; D005091:Exons / Q000235:genetics*; D006801:Humans; D006816:Huntington Disease / Q000235:genetics*; D008969:Molecular Sequence Data; D021141:Nucleic Acid Amplification Techniques; D010215:Papio; D012333:RNA, Messenger / Q000032:analysis; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D014158:Transcription, Genetic; D000099291:Adducins
D001798:Blood Proteins; D002148:Calmodulin-Binding Proteins; D012333:RNA, Messenger; D000099291:Adducins