HTLV-II-specific antisera raised in rabbits immunized with a synthetic peptide of HTLV-II envelope protein.
In order to discriminate HTLV-II from HTLV-I, HTLV-II-specific polyclonal antibodies against a synthetic peptide of HTLV-II envelope sequence were raised in rabbits. We immunized two adult rabbits with a KLH-conjugated synthetic peptide corresponding to the amino acid sequence 171-196 of the HTLV-II envelope sequence, which is a specific region for HTLV-II as evaluated with an ELISA method. The resulting rabbit antisera to the synthetic peptide reacted with gp46 of HTLV-II lysates in Western blot analysis but not with that of HTLV-I. Flow cytometric analysis and immunohistochemical study revealed that these affinity purified antisera recognized some HTLV-II-producing cell lines examined, but not HTLV-I-producing cell lines or other cell lines uninfected by HTLV. These findings indicate that these antisera specifically recognized the envelope glycoprotein (gp46) of HTLV-II and suggest the specificity of this region in the immune response to HTLV-II. Such antisera are useful in distinguishing between HTLV-I and HTLV-II infection and in determining the presence of individual HTLV-II-infected cells both in vivo and in vitro, including non-lymphoid cells. They may also assist in the elucidation of the pathogenesis of HTLV-II.
Second Department of Pathology, Okayama University Medical School.
K
K
Hayashi
N
N
Ohara
K
K
Fujiwara
H J
HJ
Jeon
T R
TR
Koirala
K
K
Takahashi
T
T
Akagi
N
N
Tomita
K
K
Miyamoto
D000595:Amino Acid Sequence; D000818:Animals; D015686:Gene Products, env / Q000235:genetics / Q000276:immunology*; D015481:HTLV-I Antibodies / Q000032:analysis* / Q000276:immunology; D015482:HTLV-II Antibodies / Q000032:analysis* / Q000276:immunology; D007114:Immunization; D008969:Molecular Sequence Data; D011817:Rabbits; D015682:Retroviridae Proteins, Oncogenic / Q000235:genetics / Q000276:immunology*; D054299:env Gene Products, Human Immunodeficiency Virus
D015686:Gene Products, env; D015481:HTLV-I Antibodies; D015482:HTLV-II Antibodies; D015682:Retroviridae Proteins, Oncogenic; D054299:env Gene Products, Human Immunodeficiency Virus; C069568:gp46 protein, Human immunodeficiency virus 2