Expression of fibroblast growth factor receptors by embryonal carcinoma cells and early mouse embryos.
We have previously shown that differentiation of embryonal carcinoma (EC) cells leads to both increased binding of FGF (fibroblast growth factor) and suppression of k-FGF expression. In the current study, we examined the expression of FGF receptors by EC cells, EC-derived differentiated cells and early mammalian embryos using the technique of reverse transcription-polymerase chain reaction (RT-PCR). We determined that both mouse, F9, and human, NT2/D1, EC cells as well as their differentiated counterparts express transcripts for two forms of FGF receptors, bek (bacterially expressed kinase) and flg (fms-like gene). In addition, we determined that mouse blastocysts express flg transcripts. The presence of FGF receptor transcripts in early embryos and the previous finding of FGF-related activity in medium conditioned by mouse blastocysts argue that the FGF family plays important roles during early mammalian development.
Campbell
W J
WJ
Eppley Institute for Cancer Research and Allied Diseases, Department of Pathology and Microbiology, Omaha, Nebraska.
Miller
K A
KA
Anderson
T M
TM
Shull
J D
JD
Rizzino
A
A
D000818:Animals; D001483:Base Sequence; D001755:Blastocyst; D002454:Cell Differentiation; D002460:Cell Line; D004622:Embryo, Mammalian; D005346:Fibroblast Growth Factors / Q000378:metabolism; D000091344:Filaggrin Proteins; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009838:Oligodeoxyribonucleotides; D016133:Polymerase Chain Reaction / Q000379:methods; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012334:RNA, Neoplasm / Q000235:genetics / Q000302:isolation & purification; D011956:Receptors, Cell Surface / Q000235:genetics* / Q000378:metabolism; D017468:Receptors, Fibroblast Growth Factor; D015183:Restriction Mapping; D013724:Teratoma; D014158:Transcription, Genetic
C000717249:FLG protein, human; D000091344:Filaggrin Proteins; D009838:Oligodeoxyribonucleotides; D012334:RNA, Neoplasm; D011956:Receptors, Cell Surface; D017468:Receptors, Fibroblast Growth Factor; D005346:Fibroblast Growth Factors; D012313:RNA