Identification of novel RFLPs in the vicinity of CpG islands in Xq28: application to the analysis of the pattern of X chromosome inactivation.
Probes for CpG islands were cloned from the distal long arm of the human X chromosome; three of them were found to be polymorphic. A HindIII RFLP was identified by the probe 2-25 (DXS606), and it was mapped to the Xq27-Xq28 boundary. Probes 2-19 (DXS605) and 2-55 (DXS707), which identify EcoRI and MspI polymorphisms, respectively, have been mapped to the distal part of Xq28, in the G6PD-RCP/GCP gene region. Probe 2-19 has been further localized about 16 kb from the 3' end of the G6PD gene. The new RFLPs may be useful for the precise mapping of the many disease genes localized in this part of the human X chromosome. Probe 2-19 is highly informative, and it has been studied in greater detail. Using the methylation-sensitive rare-cutter enzyme EagI in conjunction with the polymorphic EcoRI site, we were able to demonstrate that the RFLP may be used both to study randomness of X chromosome inactivation and for carrier detection in X-linked syndromes where nonrandom X inactivation occurs. It is conceivable that the combined use of 2-19 and of the probes described so far (pSPT-PGK and M27 beta) will make analysis of X inactivation feasible in virtually every female.
Istituto di Genetica Biochemica ed Evoluzionistica, Consiglio Nazionale Delle Richerche, Pavia, Italy.
E
E
Maestrini
S
S
Rivella
C
C
Tribioli
M
M
Rocchi
G
G
Camerino
S
S
Santachiara-Benerecetti
O
O
Parolini
L D
LD
Notarangelo
D
D
Toniolo
D000483:Alleles; D000818:Animals; D002874:Chromosome Mapping; D006224:Cricetinae; D004247:DNA / Q000235:genetics / Q000378:metabolism; D015342:DNA Probes; D015226:Dinucleoside Phosphates / Q000235:genetics*; D005260:Female; D008040:Genetic Linkage; D006801:Humans; D006822:Hybrid Cells; D008745:Methylation; D012150:Polymorphism, Restriction Fragment Length; D015183:Restriction Mapping; D014960:X Chromosome
D004247:DNA; D015342:DNA Probes; D015226:Dinucleoside Phosphates; C015772:CpG dinucleotide