Effects of various bile acids and their sodium salts on development of pepsinogen-altered pyloric glands in rats.
Effects of dietary bile acids and their sodium salts on the development of pepsinogen-altered pyloric glands (PAPG) were examined in male WKY/N Crj rats initially given a single dose of 160 mg/kg body weight of N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) by gastric intubation. From week 3 the animals were administered basal diet containing 0.5% supplements of cholic acid (CA), deoxycholic acid (DCA), chenodeoxycholic acid (CDCA) or their sodium salts (Na-C, Na-DC and Na-CDC), or 5% ascorbic acid (ASA) or its salt (Na-AS) for 18 weeks. The concentration of DCA and Na-DC was reduced to 0.3% from week 12. At week 20, animals were killed and the numbers of immunohistochemically-demonstrated PAPG were determined. Values were significantly higher with Na-C and Na-CDC than with the corresponding parent acids, and in the Na-C case PAPG development was greater than with MNNG alone. In addition, Na-CDC itself induced the numbers of PAPG significantly. These results suggest that bile salts are possible intrinsic promoters of gastric carcinogenesis. They were without effect, however, on forestomach lesions.
First Department of Pathology, Nagoya City University Medical School, Japan.
Y
Y
Yoshida
M
M
Tatematsu
K
K
Takaba
Y
Y
Shichino
N
N
Ito
D000818:Animals; D001647:Bile Acids and Salts / Q000008:administration & dosage*; D002277:Carcinoma / Q000139:chemically induced*; D004357:Drug Synergism; D008297:Male; D008769:Methylnitronitrosoguanidine / Q000008:administration & dosage*; D011708:Pylorus / Q000473:pathology; D051381:Rats; D011921:Rats, Inbred WKY; D013274:Stomach Neoplasms / Q000139:chemically induced*
D001647:Bile Acids and Salts; D008769:Methylnitronitrosoguanidine