Characterization of the RDC1 gene which encodes the canine homolog of a proposed human VIP receptor. Expression does not correlate with an increase in VIP binding sites.
We have isolated a portion of the canine gene encoding the orphan receptor RDC1 [1]. The complete coding sequence is contained in a single exon, and an intron divides the 5' untranslated region of RDC1 mRNA. The RDC1 protein is 94% homologous to the gene product of GPRN1, which has been proposed to serve as a VIP receptor when expressed in CHO-K1 and COS-7 cells (Sreedharan, S.P. et al. (1991) Proc. Natl. Acad. Sci. USA 88, 4986-4990). Northern analysis indicates that CHO-K1 cells endogenously express a 2.1 kb RDC1 mRNA. However, while CHO-K1 cells possess detectable low affinity [125I]VIP binding sites, VIP binding is not altered in membranes of CHO-K1 cells expressing varying amounts of the RDC1 gene construct. Further, endogenous VIP binding is not increased by transient expression of RDC1 in COS-7 cells. Taken together, the data suggest that RDC1 is not a canine homolog of the proposed VIP receptor.
Cook
J S
JS
Procter and Gamble Company, Corporate Research Division, Miami Valley Laboratories, Cincinnati, OH 45239-8707.
Wolsing
D H
DH
Lameh
J
J
Olson
C A
CA
Correa
P E
PE
Sadee
W
W
Blumenthal
E M
EM
Rosenbaum
J S
JS
D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D016466:CHO Cells; D002460:Cell Line; D003001:Cloning, Molecular; D006224:Cricetinae; D004285:Dogs; D019204:GTP-Binding Proteins / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D012313:RNA / Q000302:isolation & purification; D011964:Receptors, Gastrointestinal Hormone / Q000235:genetics* / Q000378:metabolism; D018005:Receptors, Vasoactive Intestinal Peptide; D012689:Sequence Homology, Nucleic Acid; D014162:Transfection; D014660:Vasoactive Intestinal Peptide
D011964:Receptors, Gastrointestinal Hormone; D018005:Receptors, Vasoactive Intestinal Peptide; D014660:Vasoactive Intestinal Peptide; D012313:RNA; D019204:GTP-Binding Proteins