DNA/RNA synthesis and labelling.
Reliable methods of machine-aided RNA synthesis have been established to complement those for DNA assembly. Oligonucleotides containing thio-modified backbones and 2'-O-alkyl sugars head the list of many newly available analogues. Biotin, fluorescent agents and many reporter groups can be conveniently introduced into oligonucleotides in multiples by phosphoramidite or H-phosphonate chemistry.
Medical Research Council, Cambridge, UK.
M J
MJ
Gait
D000818:Animals; D001710:Biotin; D004247:DNA / Q000138:chemical synthesis*; D005453:Fluorescence; D006801:Humans; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis; D009843:Oligoribonucleotides / Q000138:chemical synthesis; D012313:RNA / Q000138:chemical synthesis*
D009838:Oligodeoxyribonucleotides; D009843:Oligoribonucleotides; D012313:RNA; D001710:Biotin; D004247:DNA