Large scale cDNA sequencing for analysis of quantitative and qualitative aspects of gene expression.
Large scale sequencing of cDNAs provides a complementary approach to structural analysis of the human genome by generating expressed sequence tags (ESTs). We have initiated the large-scale sequencing of a 3'-directed cDNA library from the human liver cell line HepG2, that is a non-biased representation of the mRNA population. 982 random cDNA clones were sequenced yielding more than 270 kilobases. A significant portion of the identified genes encoded secretable proteins and components for protein-synthesis. The abundance of cDNA species varied from 2.2% to less than 0.004%. Fifty two percent of the mRNA were abundant species consisting of 173 genes and the rest were non-abundant, consisting of about 6,600 genes.
Okubo
K
K
Institute for Molecular and Cellular Biology, Osaka University, Japan.
Hori
N
N
Matoba
R
R
Niiyama
T
T
Fukushima
A
A
Kojima
Y
Y
Matsubara
K
K
D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D015870:Gene Expression; D005787:Gene Frequency; D015723:Gene Library; D015894:Genome, Human; D006801:Humans; D008099:Liver / Q000737:chemistry* / Q000166:cytology; D008969:Molecular Sequence Data; D011506:Proteins / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D014407:Tumor Cells, Cultured
D018076:DNA, Complementary; D011506:Proteins; D012333:RNA, Messenger