Effects of intravenous bicuculline and strychnine on inspiratory inhibitory responses in the cat.
Single shock stimulation of the superior laryngeal nerve (SLN), intercostal nerve (ICN), phrenic nerve (PN) or within the medullary respiratory groups (DRG-VRG) produces a transient, short-latency attenuation of inspiratory motor activity. Trains of stimuli delivered to SLN and ICN cause premature termination of inspiration. This study examined involvement of glycine and GABAA receptors in these reflex inhibitions. Experiments were conducted in decerebrate, vagotomized, and paralyzed cats. Control responses of left PN activity to threshold single shock stimulation of SLN, PN, ICN and the DRG-VRG were recorded and the thresholds for SLN- and ICN-evoked inspiratory termination were determined. Five min after intravenous injection of bicuculline (1 mg/kg) or strychnine (50 micrograms/kg), the responses to stimulation were again recorded. This procedure was reiterated until the cumulative dose elicited marked convulsions. Neither drug affected the inspiratory terminating reflexes. Systemic bicuculline had no effect on transient inspiratory inhibition. However strychnine prolonged the onset latency and the duration of all four inhibitory responses. Since the degree of transient inhibition was not lessened (only delayed), it appears that these inspiratory inhibitory reflexes do not rely exclusively on actions of glycine or GABAA receptors.
Department of Physiology, University of Kentucky Medical Center, Lexington 40536-0084.
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D000818:Animals; D001640:Bicuculline / Q000494:pharmacology*; D001933:Brain Stem / Q000502:physiology; D002415:Cats; D003655:Decerebrate State / Q000503:physiopathology; D004558:Electric Stimulation; D007275:Injections, Intravenous; D007823:Laryngeal Nerves / Q000502:physiology; D009475:Neurons, Afferent / Q000187:drug effects / Q000502:physiology; D018377:Neurotransmitter Agents / Q000502:physiology; D010791:Phrenic Nerve / Q000502:physiology; D015656:Respiratory Mechanics / Q000187:drug effects*; D013331:Strychnine / Q000494:pharmacology*; D014628:Vagotomy
D018377:Neurotransmitter Agents; D013331:Strychnine; D001640:Bicuculline