Generation of 19 STS markers that can be anchored at specific sites on human chromosome 21.
Sequence-tagged sites (STSs) are short stretches of DNA that can be specifically detected by the polymerase chain reaction (PCR) and can be used to construct long-range physical maps of chromosomal DNA. These STSs can be detected by PCR assays developed by reference to data obtained from the sequencing of restriction fragment length polymorphism-DNA markers for chromosome 21, which were derived from recombinant lamba-phage and plasmid clones made from DNA of a human-hamster hybrid cell line. In this report, we describe the generation of 19 new STSs that are specific for human chromosome 21.
Gene Bank, Tsukuba Life Science Center, RIKEN, Institute of Physical and Chemical Research, Ibaraki, Japan.
X
X
Tang
H
H
Tashiro
T
T
Eki
Y
Y
Murakami
E
E
Soeda
T
T
Sakakura
P C
PC
Watkins
K
K
Yokoyama
D001483:Base Sequence; D002874:Chromosome Mapping; D002891:Chromosomes, Human, Pair 21; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D006822:Hybrid Cells; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D016324:Sequence Tagged Sites