Production of specific antibody against L-dopa and its ultrastructural localization of immunoreactivity in the house-shrew (Suncus murinus) lateral habenular nucleus.
An antiserum was raised against L-DOPA bound to bovine serum albumin, purified by affinity chromatography, and its specificities were verified by immunoblotting and enzyme-linked immunosorbent assays. The antiserum did not cross-react with dopamine (DA), tyrosine, tyramine, octopamine, norepinephrine or epinephrine. Immunocytochemical studies using the PAP method revealed that tyrosine hydroxylase- and L-DOPA positive but aromatic L-amino acid decarboxylase- and DA-negative neurons were present in the lateral habenular nucleus of the house-shrew (Suncus murinus) brain. Ultrastructurally L-DOPA immunoreactive products were localized in the cytoplasmic matrix and terminals with vesicles.
Department of Anatomy, School of Medicine, Fujita Health University, Aichi, Japan.
N
N
Karasawa
G
G
Isomura
I
I
Nagatsu
D000818:Animals; D000918:Antibody Specificity; D001142:Aromatic-L-Amino-Acid Decarboxylases / Q000032:analysis; D007124:Immunoenzyme Techniques; D007980:Levodopa / Q000276:immunology*; D009419:Nerve Tissue Proteins / Q000032:analysis; D011817:Rabbits; D012788:Shrews / Q000378:metabolism*; D013787:Thalamic Nuclei / Q000737:chemistry* / Q000648:ultrastructure; D014446:Tyrosine 3-Monooxygenase / Q000032:analysis
D009419:Nerve Tissue Proteins; D007980:Levodopa; D014446:Tyrosine 3-Monooxygenase; D001142:Aromatic-L-Amino-Acid Decarboxylases