Immunoadsorption: strategies for antigen elution and production of reusable adsorbents.
Immunoadsorption is a powerful and generalizable method for protein purification that exploits the fine specificity of antigen-antibody interactions. In spite of its potential utility, the more widespread process scale use of immunoadsorption has been limited by the high cost of the antibody and the lack of gentle elution schemes that completely preserve the activity of both the immunoadsorbent and the eluted product. In this report, we review common chemical elution strategies such as pH, ionic strength, chaotropic salts, denaturants, and organic solvents as well as physical techniques such as pressure, electrokinetics, and temperature. In general, selection of elution strategies has largely been an empirical art, balancing stability of the immunoadsorbent and the eluted product and efficiency. The limitations of the available choices demonstrate that more attention must be placed on the antibody. Techniques which assist in the identification or creation of new antibodies with improved binding properties and resistance to degradation, e.g., screening and/or rational protein engineering, are also discussed.
Department of Chemical and Biochemical Engineering, Rutgers University, Piscataway, New Jersey.
M L
ML
Yarmush
K P
KP
Antonsen
S
S
Sundaram
D M
DM
Yarmush
D000941:Antigens; D001709:Biotechnology / Q000379:methods*; D007163:Immunosorbent Techniques; D007164:Immunosorbents
D000941:Antigens; D007164:Immunosorbents