Human granulosa-luteal and cumulus cells express transforming growth factors-beta type 1 and type 2 mRNA.
Human granulosa-luteal cells and cumulus cells obtained from women undergoing in vitro fertilization and embryo transfer (IVF-ET) were examined for the presence of TGF-beta 1 and TGF-beta 2 mRNA by reverse transcription-polymerase chain reaction (RT-PCR) analysis. RT-PCR analysis revealed that both follicle cell types express mRNA for both TGF-beta subtypes. Verification of RT-PCR products was done by restriction enzyme digestion analysis. These results suggest a role(s) for TGF-beta 1 and TGF-beta 2 in the development of human granulosa-luteal cells and the oocyte-cumulus cell complex.
Department of Obstetrics/Gynecology, Duke University Medical Center, Durham, NC 27710.
G W
GW
Mulheron
N L
NL
Bossert
J A
JA
Lapp
D K
DK
Walmer
D W
DW
Schomberg
D001483:Base Sequence; D003338:Corpus Luteum / Q000502:physiology*; D004624:Embryo Transfer; D005260:Female; D005307:Fertilization in Vitro; D006107:Granulosa Cells / Q000502:physiology*; D006801:Humans; D008596:Menotropins / Q000627:therapeutic use; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D010053:Ovary / Q000166:cytology / Q000502:physiology*; D016133:Polymerase Chain Reaction / Q000379:methods; D012313:RNA / Q000302:isolation & purification; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D013480:Superovulation; D016212:Transforming Growth Factor beta / Q000235:genetics*
D009838:Oligodeoxyribonucleotides; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta; D008596:Menotropins; D012313:RNA