The sensitive determination of abanoquil in blood by high-performance liquid chromatography/atmospheric pressure ionization mass spectrometry.
A method is described for the determination of abanoquil in human blood. The method is based on high-performance liquid chromatography (HPLC)/atmospheric pressure positive ion chemical ionization mass spectrometry, using (2H3)abanoquil as internal standard. Multiple reaction monitoring is employed for selectivity and sensitivity, which enables quantification over the range 10-500 pg ml-1 with acceptable precision and accuracy. This assay methodology illustrates the versatility of atmospheric pressure ionization/tandem mass spectrometry, in conjunction with HPLC, for the separation and quantification of drugs in the subnanogram per millilitre range.
Department of Drug Metabolism Pfizer Central Research, Sandwich, Kent, UK.
B
B
Kaye
M W
MW
Clark
N J
NJ
Cussans
P V
PV
Macrae
D A
DA
Stopher
D000317:Adrenergic alpha-Antagonists / Q000097:blood*; D000634:Aminoquinolines / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D066298:In Vitro Techniques; D013058:Mass Spectrometry / Q000379:methods; D044005:Tetrahydroisoquinolines
D000317:Adrenergic alpha-Antagonists; D000634:Aminoquinolines; D044005:Tetrahydroisoquinolines; C058534:abanoquil