Dried gel hybridization in place of southern hybridization for detection of Listeria monocytogenes DNA fragments.
A simple, sensitive, dried gel DNA hybridization method for detection of Listeria monocytogenes DNA fragments is described. DNA samples were fractionated on an agarose gel. The gel was then denatured in NaOH-NaCl and neutralized in Tris-NaCl. The resulting agarose gel was dried and hybridized with 32P-labelled DNA probe. No transfer to nitrocellulose membranes was used.
Wang
R F
RF
Department of Food Science, University of Arkansas, Fayetteville 72703.
Cao
W W
WW
Johnson
M G
MG
D015139:Blotting, Southern; D004269:DNA, Bacterial / Q000032:analysis*; D004587:Electrophoresis, Agar Gel; D008089:Listeria monocytogenes / Q000235:genetics*; D009693:Nucleic Acid Hybridization
D004269:DNA, Bacterial