Thy-1 antigen-mediated adhesion of mouse lymphoid cells to stromal cells of haemopoietic origin.
Thy-1 antigen is one of the widespread cell surface antigens. The antigen is expressed in the cells of a wide variety of different tissues and species and its pattern varies considerably during development and among species. Although the Thy-1 is one of the members of Ig-superfamily its function(s) remain unknown. Here we show that monoclonal antibodies to mouse Thy-1.2 antigen prevent adhesion of Thy-1.2-positive mouse T-leukemic cells 127 to monolayers of two mouse bone marrow stromal cell lines (14F1.1 and MBA-15). The same antibodies do not prevent adhesion of cell 127 to monolayers of two other mouse bone marrow stromal cell lines (MBA-2.1 and MBA-1.1.1) and to a wide spectrum of different normal and tumor mouse cell lines. A characteristic of the cell line 14F1.1 is the ability to support growth of mouse haemopoietic stem-cells. This data suggest that cells of stromal lines 14F1.1 and MBA-15 contain on their surface a specific ligand for the Thy-1 molecule. Cell-cell interaction via this ligand and the molecule on the cell surface may be an important step for development of certain lineages of cells.
Department of Chemical Immunology, Weizmann Institute of Science, Rehovot, Israel.
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Irlin
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Peled
D000818:Animals; D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D001853:Bone Marrow / Q000276:immunology; D001854:Bone Marrow Cells; D002448:Cell Adhesion / Q000276:immunology*; D002450:Cell Communication / Q000276:immunology; D002460:Cell Line; D008562:Membrane Glycoproteins; D051379:Mice; D015398:Signal Transduction / Q000276:immunology; D013601:T-Lymphocytes / Q000166:cytology* / Q000276:immunology*; D018800:Thy-1 Antigens
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D008562:Membrane Glycoproteins; D018800:Thy-1 Antigens