Monoclonal antibodies to bis-indole alkaloids of Catharanthus roseus and their use in enzyme-linked immuno-sorbent-assays.
High affinity monoclonal antibodies directed against bis-indole alkaloids from Catharanthus roseus were produced. Once characterized, they were used to develop sensitive enzyme-linked-immuno-sorbent-assays, enabling us to determine minute quantities of alkaloids related to vinblastine in plant material.
Laboratoire de Botanique et Phytochimie, Faculté de Pharmacie, Châtenay-Malabry, France.
M C
MC
Cibotti
C
C
Freier
J
J
Andrieux
M
M
Plat
L
L
Cosson
C
C
Bohuon
D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D010944:Plants / Q000032:analysis / Q000276:immunology; D014747:Vinblastine / Q000276:immunology; D014748:Vinca Alkaloids / Q000032:analysis / Q000276:immunology*
D000911:Antibodies, Monoclonal; D014748:Vinca Alkaloids; D014747:Vinblastine