Restriction fragment length polymorphism and virulence pattern of the veterinary pathogen Escherichia coli O139:K82:H1.
Escherichia coli O139:K82:H1 strains originating from outbreaks and single cases of oedema disease in pigs were characterized by their genomic restriction fragment length polymorphism (RFLP), their virulence pattern, and by the occurrence as well as the genomic distribution of the determinants for hemolysin (hly) and verotoxins (shiga-like toxins; sltI, sltII). Whereas the RFLPs revealed considerable variation among the E. coli O139:K82:H1 isolates depending the origin and epidemic source of the strains, the virulence gene slt II was found to be present in nearly all strains in a particular chromosomal region. Similar to RFLPs, the plasmid profiles are useful for epidemiological analysis.
BGA, Robert Koch-Institut, Wernigerode.
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Tschäpe
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Bender
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Ott
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Wittig
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J
Hacker
D000818:Animals; D001427:Bacterial Toxins / Q000235:genetics; D003603:Cytotoxins / Q000235:genetics; D004269:DNA, Bacterial / Q000032:analysis; D004488:Edema Disease of Swine / Q000382:microbiology*; D004926:Escherichia coli / Q000145:classification / Q000235:genetics / Q000472:pathogenicity*; D004927:Escherichia coli Infections / Q000382:microbiology*; D006460:Hemolysin Proteins / Q000235:genetics; D010641:Phenotype; D012150:Polymorphism, Restriction Fragment Length; D022622:Shiga Toxin 1; D022641:Shiga Toxin 2; D013552:Swine; D014774:Virulence
D001427:Bacterial Toxins; D003603:Cytotoxins; D004269:DNA, Bacterial; D006460:Hemolysin Proteins; D022622:Shiga Toxin 1; D022641:Shiga Toxin 2