Evaluation of the physiological value of porcine luteal cells isolated in various stages of the luteal phase: tissue culture approach.
Porcine luteal cells were collected from corpora lutea in four different stages of the luteal phase and cultured as monolayers. Progesterone (P4) secretion was assayed using radioimmunoassays (Gregoraszczuk, 1991). Luteal cells cultured from porcine corpora lutea collected in the early luteal phase maintained steroidogenic capacity for 6 days in culture until the time comparable with midluteal corpora lutea. Luteal cells collected from mature and regressing corpora lutea did not dedifferentiate during 2 days of culture. After this time secretion of progesterone decreased to undetectable amounts characteristic of old corpora lutea. The regression in the culture progressed. The results demonstrate that the degree of the decline of progesterone depends on the type of corpus luteum, which is connected to particular time intervals of the luteal phase. Before starting experiments it is necessary to take into consideration the stage of the luteal phase from which the material is collected for culture. This study provides evidence that long term culture is useful for investigating a variety of aspects of luteal function only if cells are collected in the early luteal phase. Short term culture is suitable for investigation of cells collected from mid and late luteal phase. Regulation of luteal function is dependent on stage of the luteal phase.
Department of Physiology, Jagiellonian University, Kraków, Ingardena, Poland.
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Gregoraszczuk
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Wojtusiak
D000818:Animals; D002469:Cell Separation; D002470:Cell Survival; D002478:Cells, Cultured; D003338:Corpus Luteum / Q000166:cytology* / Q000378:metabolism / Q000502:physiology; D005260:Female; D008183:Luteal Phase; D011374:Progesterone / Q000378:metabolism; D013552:Swine
D011374:Progesterone