Chromosomal mapping of the human annexin IV (ANX4) gene.
Annexin IV (placental anticoagulant protein II) is a member of the annexin or lipocortin family of calcium-dependent phospholipid-binding proteins. A cDNA for human annexin IV was isolated from a placental library that is 675 bases longer in the 3' untranslated region than previously reported, indicating the existence of alternative mRNA processing for this gene. Genomic Southern blotting with a cDNA probe indicated a gene size of 18-56 kb. Primers developed for polymerase chain reaction (PCR) allowed amplification of a 1.6-kb portion of the ANX4 gene. DNA sequence analysis showed that this PCR product contained a single intron with exon-intron boundaries in exactly the same position as in the mouse annexin I and annexin II genes. PCR analysis of a somatic cell hybrid panel mapped the ANX4 gene to chromosome 2, and in situ hybridization with a cDNA probe showed a unique locus for ANX4 at 2p13. This study provides further evidence that genes for the annexins are dispersed throughout the genome but are similar in size and exon-intron organization.
Department of Laboratory Medicine, University of Washington, Seattle 98195.
J F
JF
Tait
C
C
Smith
D A
DA
Frankenberry
C H
CH
Miao
D A
DA
Adler
C M
CM
Disteche
D000595:Amino Acid Sequence; D000818:Animals; D017302:Annexins; D001483:Base Sequence; D002874:Chromosome Mapping; D002889:Chromosomes, Human, Pair 2; D004247:DNA / Q000235:genetics; D015342:DNA Probes; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D012150:Polymorphism, Restriction Fragment Length; D011257:Pregnancy Proteins / Q000235:genetics*
D017302:Annexins; D015342:DNA Probes; D011257:Pregnancy Proteins; D004247:DNA