The biosynthesis of polyunsaturated fatty acids by rat sertoli cells.
1. The biosynthesis of polyunsaturated fatty acids (PUFA) of the n-6 and n-3 series was investigated in cultured Sertoli cells. 18:2n-6, 18:3n-6, 20:2n-6, 18:3n-3 and 20:3n-3 were added individually at a concentration of 20 mumol to culture media. 2. Maximum incorporation of 20- and 22-carbon PUFA into membrane lipids was observed after 72 hr of incubation with all the exogenous substrates used. 3. As reported in other cell systems, the delta 6 desaturation was the first rate-limiting step; the major factor regulating this activity was the concentration of linoleic acid or alpha-linolenic acid in the medium. 4. Our data show that the delta 5-desaturation represents a second regulatory step in PUFA biosynthesis. 5. The sum of n-6 and n-3 PUFA of the 22 carbon chain length constantly represented between 11 and 12% of total fatty acids, regardless of the exogenous substrate used. 6. Our kinetic studies of the incorporation of PUFA of the n-6 and n-3 series did not permit detection of a delta 8 desaturase activity.
Laboratoire de Biochimie, URA CNRS 609, Université de Caen, France.
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Oulhaj
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Huynh
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Nouvelot
D000818:Animals; D002478:Cells, Cultured; D003470:Culture Media; D004537:Eicosanoic Acids / Q000494:pharmacology; D005231:Fatty Acids, Unsaturated / Q000032:analysis / Q000096:biosynthesis*; D007700:Kinetics; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000494:pharmacology; D008042:Linolenic Acids / Q000494:pharmacology; D008297:Male; D051381:Rats; D012708:Sertoli Cells / Q000378:metabolism*; D017962:alpha-Linolenic Acid
D003470:Culture Media; D004537:Eicosanoic Acids; D005231:Fatty Acids, Unsaturated; D008041:Linoleic Acids; D008042:Linolenic Acids; D017962:alpha-Linolenic Acid; D019787:Linoleic Acid