[Beneficial effects of perfusion of atrial natriuretic factor in acute post-ischemic renal insufficiency in the rat].
Owing to its capacity of increasing glomerular filtration, potential beneficial effects of atrial natriuretic factor (ANF) were assessed during a post ischemic acute renal insufficiency in rats. Renal insufficiency was obtained by clamping the renal artery during 30 min., and by performing a reperfusion during 2 hours in uninephrectomized rats. Three groups were defined: a control group where animals were submitted to an operation procedure without renal artery clamping, a control group were animals received a physiological serum perfusion (1.5 ml/h) during the renal reperfusion time and an experimental group where animals were administrated a rat 1-28 ANF perfusion (5 microns/ml in NaCl 0.9%, 1.5 ml/hour) during the reperfusion time. Insulin clearance (1.0 + 0.05 ml/h vs 0.7 + 0.05 ml/h, p < 0.01), and diuresis (32.9 +/- 3.6 microliters/min. vs 7.5 +/- 0.23 microliters/min., p < 0.01) were significantly higher in rats which were administrated a NaCl 0.9% perfusion. Histologically, a significant decrease in kidney weight and in he percentage of diseases nephrons was observed after reperfusion in ANF treated rats. The results obtained demonstrate that ANF perfusion in case of post ischemic acute renal insufficiency in rats improves the recovery of renal function and reduces the renal histologic lesions.
Hôpital Broussais, Paris.
F
F
Haab
D
D
Farge
D
D
Nochy
I
I
Viossat
M
M
Cambillaut
J N
JN
Fabiani
D058186:Acute Kidney Injury / Q000209:etiology / Q000628:therapy*; D000818:Animals; D009320:Atrial Natriuretic Factor / Q000008:administration & dosage* / Q000494:pharmacology; D003250:Constriction; D003404:Creatinine / Q000097:blood; D004231:Diuresis / Q000187:drug effects; D005919:Glomerular Filtration Rate / Q000187:drug effects; D007262:Infusions, Intravenous; D007511:Ischemia / Q000150:complications; D007668:Kidney / Q000098:blood supply / Q000187:drug effects; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012077:Renal Artery; D015427:Reperfusion Injury / Q000503:physiopathology
C472610:atrial natriuretic peptide, rat; D009320:Atrial Natriuretic Factor; D003404:Creatinine
Effets bénéfiques de la perfusion du facteur natriurétique auriculaire au cours de l'insuffisance rénale aiguë post-ischémique chez le rat.