Immunohistochemical markers in rat cortex: co-localization of calretinin and calbindin-D28k with neuropeptides and GABA.
Calretinin and calbindin-D28k are two calcium-binding proteins which are present in separate populations of interneurons in cerebral cortex and hippocampus. To identify these cells with the populations expressing different transmitters, two-colour immunofluorescence was done with antibodies against the calcium-binding proteins plus antibodies against vasoactive intestinal peptide (VIP), somatostatin (SRIF), or gamma-aminobutyric acid (GABA). In neocortex, calretinin is partially co-localized with VIP (especially in the deeper layers) and is not co-localized with SRIF. Calbindin is largely co-localized with SRIF, and not with VIP. Both calretinin and calbindin are partially co-localized with GABA. In piriform and entorhinal cortex, the patterns resemble those in neocortex. In hippocampus, preliminary data indicate greater heterogeneity, especially in the ventral part; at least a few double-positive cells are present for every combination of calcium-binding protein and neuropeptide. These results expand the known diversity of local-circuit neurons in cortical regions.
Department of Physiology, University of Cambridge, UK.
J H
JH
Rogers
D000818:Animals; D064092:Calbindin 1; D064032:Calbindin 2; D064026:Calbindins; D002540:Cerebral Cortex / Q000033:anatomy & histology / Q000378:metabolism*; D006624:Hippocampus / Q000276:immunology / Q000378:metabolism; D007150:Immunohistochemistry; D009479:Neuropeptides / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D064030:S100 Calcium Binding Protein G / Q000378:metabolism*; D013004:Somatostatin / Q000276:immunology / Q000378:metabolism; D014660:Vasoactive Intestinal Peptide / Q000276:immunology / Q000378:metabolism; D005680:gamma-Aminobutyric Acid / Q000276:immunology / Q000378:metabolism*
C576500:Calb1 protein, rat; C576512:Calb2 protein, rat; D064092:Calbindin 1; D064032:Calbindin 2; D064026:Calbindins; D009479:Neuropeptides; D064030:S100 Calcium Binding Protein G; D014660:Vasoactive Intestinal Peptide; D013004:Somatostatin; D005680:gamma-Aminobutyric Acid