Nerve-dependent expression of c-myc protein during forelimb regeneration of Xenopus laevis froglets.
The consequences of denervation on the expression of c-myc protein have been analyzed on the regenerating forelimb of young froglets of Xenopus laevis. The level of c-myc expression, low in control limbs and enhanced in the regenerate, is transiently increased after a three-hour total denervation. For this protein, the level of expression is not a function of the quantity of nerve in the regenerate. Four days after denervation, c-myc signal is back to its base level observed in the regenerate. A different pattern of expression is obtained for an S phase marker (PCNA protein) taken as a control in the same experimental conditions. The data presented here show that the nervous system normally exerts a negative control on the expression of c-myc and PCNA proteins in the limb regenerate of Xenopus.
Lemaître
J M
JM
Institut Jacques Monod, Université Paris VII-CNRS, France.
Méchali
M
M
Géraudie
J
J
D000818:Animals; D003714:Denervation; D005552:Forelimb / Q000254:growth & development / Q000294:innervation*; D005786:Gene Expression Regulation; D009687:Nuclear Proteins / Q000096:biosynthesis*; D010751:Phosphopyruvate Hydratase / Q000096:biosynthesis; D018809:Proliferating Cell Nuclear Antigen; D016271:Proto-Oncogene Proteins c-myc / Q000096:biosynthesis*; D012038:Regeneration; D014982:Xenopus laevis
D009687:Nuclear Proteins; D018809:Proliferating Cell Nuclear Antigen; D016271:Proto-Oncogene Proteins c-myc; D010751:Phosphopyruvate Hydratase