A new serotype of Bacillus thuringiensis from Colombia toxic to mosquito larvae.
During a survey conducted in Colombia a new isolate of Bacillus thuringiensis that showed toxicity toward Culex quinquefasciatus, Cx. pipiens, Aedes aegypti, and Anopheles stephensi larvae was isolated. Parasporal crystals were spherical in shape and showed a great degree of similarity with those produced by the reference strain of Bacillus thuringiensis subsp. israelensis. Supernatant fraction of the whole culture was not toxic, and heat-stable exotoxin production was negative. Catalase, urease, arginine dihydrolase, amylase, lecithinase, acetyl-methyl-carbinol, and gelatinase production were positive. Hemolysis on sheep blood agar was alpha-type. The isolate 163-131 showed natural resistance to azolocillin and was sensible to cephoperazone, cephalotin, nalidixic acid, and trimetoprin sulfametoxazole. Flagellar agglutination showed a specific H 30 antigen which allows individualization of this strain as a new serotype and the subspecies name of medellin is proposed.
Biological Control Section, Corporacion para Investigaciones Biologicas, H.P.T.U., Medellin, Colombia, South America.
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Orduz
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Rojas
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MM
Correa
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AE
Montoya
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H
de Barjac
D000818:Animals; D001413:Bacillus thuringiensis / Q000145:classification / Q000502:physiology*; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D003105:Colombia; D009033:Culicidae / Q000382:microbiology*; D004731:Endotoxins; D006460:Hemolysin Proteins; D007814:Larva / Q000382:microbiology; D010572:Pest Control, Biological; D013171:Spores, Bacterial
D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004731:Endotoxins; D006460:Hemolysin Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis