A method for the preparation of coimmobilizates by adhesion using polyethylenimine.
A method has been described for obtaining coimmobilizates by the simultaneous binding of glucose oxidase to the cell and the enzyme-bound cell to cotton thread through adhesion using polyethylenimine (PEI). Glucose oxidase was found to adsorb onto PEI-coated yeast cells from a water suspension. The desorption observed at higher ionic strength could be obviated by cross-linking with 2% glutaraldehyde for 2 min. The enzyme-bound yeast cells could then be immobilized by adhesion on cotton thread. The coimmobilizate could be reused for over 10 batches without appreciable loss in activity.
D'Souza
S F
SF
Food Technology and Enzyme Engineering Division, Bhabha Atomic Research Centre, Bombay, India.
Melo
J S
JS
D004800:Enzymes, Immobilized / Q000378:metabolism; D005949:Glucose Oxidase / Q000378:metabolism*; D007700:Kinetics; D011094:Polyethyleneimine; D011485:Protein Binding; D012441:Saccharomyces cerevisiae / Q000378:metabolism*
D004800:Enzymes, Immobilized; D011094:Polyethyleneimine; D005949:Glucose Oxidase