[Interaction of a mixture of epimastigotes and tripomastigotes from a culture of a Mexican isolate of Trypanosoma cruzi with mouse macrophages].
Chagas disease, a cause of important morbo-lethality in Latinamerica, is produced by a protozoan which has a circulating and tissular phase the Trypanosoma cruzi. Adhesion between T. cruzi and phagocytes is the first step in cellular parasitism, which has a central role in pathogenesis. Although there are studies on parasite phagocyte interaction with some strains of T. cruzi, and it is known that biological variation does exist. We now report the first studies with a mexican isolate using a mix of different phases of T. cruzi obtained from acellular culture resembling the natural conditions. Murine macrophages adheres to epimastigotes, transitional trypomastigotes and metacyclic trypomastigotes since the first minutes of observation, progression on cell-cell interaction was demonstrated, there were no differences among parasite faces however, in some cases there was a failed adhesion, this suggest a possible parasite evasion mechanism. These studies limited only to morphologic aspects the adhesion phenomena, should be pursued.
Departamento de Inmunología, Instituto Nacional de Cardiología Ignacio Chávez.
V M
VM
Monteon
V
V
Nekrassov
A
A
Zepeda
A
A
Aranda
P A
PA
Reyes
D000818:Animals; D014355:Chagas Disease / Q000469:parasitology*; D006801:Humans; D008262:Macrophage Activation; D017737:Macrophages, Peritoneal / Q000502:physiology*; D008800:Mexico; D051379:Mice; D008855:Microscopy, Electron, Scanning; D010587:Phagocytosis; D000267:Tissue Adhesions; D014349:Trypanosoma cruzi / Q000254:growth & development / Q000302:isolation & purification / Q000502:physiology*
Interacción de una mezcla de epimastigotes y tripomastigotes de cultivo de un aislamiento mexicano de Trypanosoma cruzi con macrófagos murinos.