Establishment and characterization of monoclonal antibodies against Chuzan virus K-47.
We established sixteen mouse monoclonal antibodies reactive to Chuzan virus K-47 strain using P3-X63-Ag8-U1 cells as fusion partner cells. Among them, CG53/2/4 recognized a 100K structural protein of the virus. The 100K antigen lost it's antigenicity for CG53/2/4 after mild periodate oxidation treatment, suggesting that the 100K viral antigen is a glycoprotein. In addition, CG53/2/4 neutralized the viral infectivity. This indicates that the 100K glycoprotein is essential for the infection of the virus. The other monoclonal antibodies reacted with a 41K antigen of the virus. Especially CG1/1 showed the highest reactivity to the virus. Forward step sandwich assay using CG1/1 and biotinylated CG53/2/4 could detect the virus at 10TCID50/ml. Therefore, these monoclonal antibodies can eventually predict the virus infection to the animals before their sideration.
Eto
N
N
Department of Food Science and Technology, Faculty of Agriculture, Kyushu University, Fukuoka, Japan.
Yamada
K
K
Koga
A
A
Shirahata
S
S
Murakami
H
H
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000956:Antigens, Viral / Q000276:immunology; D001710:Biotin; D002460:Cell Line; D003429:Cross Reactions; D004285:Dogs; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009500:Neutralization Tests; D010504:Periodic Acid; D012087:Reoviridae / Q000276:immunology*; D015678:Viral Structural Proteins / Q000502:physiology
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D015678:Viral Structural Proteins; D010504:Periodic Acid; D001710:Biotin