Fibrin glue from single-donation autologous plasmapheresis.
A method is described for preparing fibrin glue from fibrinogen obtained by double cryoprecipitation of plasma collected by plasmapheresis. Plasma (average volume, 528.8 +/- 86 mL), collected from 12 plasmapheresis donors was cryoprecipitated twice. When the cryoprecipitated pellet was resuspended in 0.5 to 2 mL of saline (total volume of saline-resuspended fibrinogen: 5 mL), the average yield of fibrinogen was 78.4 +/- 18.3 mg per mL. This is comparable to commercial preparations, is suitable for clinical use, and offers greater safety at a reduced cost.
Casali
B
B
Centro Trasfusionale, Ospedale S. Maria, Reggio Emilia, Italy.
Rodeghiero
F
F
Tosetto
A
A
Palmieri
B
B
Immovilli
R
R
Ghedini
C
C
Rivasi
P
P
D004591:Electrophoresis, Polyacrylamide Gel; D005176:Factor XIII / Q000032:analysis; D015718:Fibrin Tissue Adhesive / Q000737:chemistry; D005340:Fibrinogen / Q000032:analysis; D005353:Fibronectins / Q000032:analysis; D006801:Humans; D046911:Macromolecular Substances; D008722:Methods; D010956:Plasmapheresis; D010958:Plasminogen / Q000032:analysis; D012709:Serum Albumin / Q000032:analysis; D011503:Transglutaminases / Q000378:metabolism
D015718:Fibrin Tissue Adhesive; D005353:Fibronectins; D046911:Macromolecular Substances; D012709:Serum Albumin; D005340:Fibrinogen; D010958:Plasminogen; D005176:Factor XIII; D011503:Transglutaminases