Expression of c-sis and c-fos genes in human meningiomas and neurinomas.
The HindIII c-sis RFLP was analyzed in constitutional and tumor DNAs of 19 meningioma patients. Loss of a c-sis allele (allele I in all cases) was observed in 6 out of 10 tumors from heterozygous patients. No alteration in the structure or dosage of the c-sis gene was detected in any tumor sample. Northern analysis of c-sis evidenced sis transcripts in 7 out of 8 meningiomas which were amenable to analysis. All tumors expressing c-sis also expressed c-fos, suggesting that the autocrine loop elicited by sis activation may have c-fos as its nuclear target. Co-expression of c-sis and c-fos was also observed in 5 neuromas out of 7 analyzed. Very high levels of fos-mRNA were observed in 2 neuromas, but were not found to be caused by apparent alteration or amplification of the c-fos gene.
Department of Biology and Genetics, Medical Faculty, University of Milan, Italy.
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Larizza
D000293:Adolescent; D000328:Adult; D000368:Aged; D015139:Blotting, Southern; D004273:DNA, Neoplasm / Q000235:genetics / Q000302:isolation & purification; D015247:Deoxyribonuclease HindIII; D005260:Female; D016762:Genes, fos; D006801:Humans; D008297:Male; D008577:Meningeal Neoplasms / Q000235:genetics*; D008579:Meningioma / Q000235:genetics*; D008875:Middle Aged; D009442:Neurilemmoma / Q000235:genetics*; D009464:Neuroma, Acoustic / Q000235:genetics*; D010982:Platelet-Derived Growth Factor / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D020574:Proto-Oncogene Proteins c-sis; D011519:Proto-Oncogenes; D012334:RNA, Neoplasm / Q000235:genetics / Q000302:isolation & purification; D013120:Spinal Cord Neoplasms / Q000235:genetics*
D004273:DNA, Neoplasm; D010982:Platelet-Derived Growth Factor; D011518:Proto-Oncogene Proteins; D020574:Proto-Oncogene Proteins c-sis; D012334:RNA, Neoplasm; D015247:Deoxyribonuclease HindIII