Semi-automated solid-phase DNA sequencing.
Increasing the efficiency of DNA sequencing necessitates the development of systems which reduce the need for manual operations by integrating template preparation, sequencing reactions, product separation and detection. A semi-automated system, whereby PCR-amplified biotinylated genomic or plasmid DNA is immobilized on streptavidin-coated magnetic beads, has been developed.
Department of Biochemistry and Biotechnology, Royal Institute of Technology, Stockholm, Sweden.
M
M
Uhlén
T
T
Hultman
J
J
Wahlberg
J
J
Lundeberg
S
S
Bergh
B
B
Pettersson
A
A
Holmberg
S
S
Ståhl
T
T
Moks
D001331:Automation; D001483:Base Sequence; D003365:Costs and Cost Analysis; D004247:DNA; D010957:Plasmids; D016133:Polymerase Chain Reaction / Q000379:methods
D004247:DNA