Rat model to investigate the treatment of acute nitrogen dioxide intoxication.
1. The pulmonary toxic events induced by acute nitrogen dioxide (NO)2 exposure were studied in the rat to develop an inhalation model to investigate therapeutic measures. 2. A good correlation was observed between the lung weights and severity of the atypical pneumonitis. The pulmonary effects observed, became more pronounced with increasing NO2 concentrations (0, 25, 75, 125, 175 or 200 ppm, 1 ppm NO2 = 1.88 mg m-3 NO2) and exposure times (5, 10, 20 or 30 min). 3. An adequate NO2 concentration is 175 ppm, because it can induce a severe lung injury without mortality. This makes it possible to investigate suitable therapeutic interventions for several days. 4. Following acute inhalatory NO2 intoxication, transformation of NO2 to nitrate is presumably more notable than transformation to nitrite. 5. The transformation of NO2 to nitrate in lung tissue causes a slight increase in the serum nitrite concentration, which does not induce measurable formation of methaemoglobin. 6. Presumably, methaemoglobin does not contribute to the toxicity of NO2 intoxication.
National Poison Control Centre, National Institute of Public Health and Environmental Protection, Bilthoven, The Netherlands.
J
J
Meulenbelt
J A
JA
Dormans
M
M
Marra
P J
PJ
Rombout
B
B
Sangster
D000280:Administration, Inhalation; D000818:Animals; D001273:Atmosphere Exposure Chambers; D001711:Biotransformation; D001835:Body Weight / Q000187:drug effects; D005260:Female; D008168:Lung / Q000187:drug effects* / Q000473:pathology; D008954:Models, Biological; D009585:Nitrogen Dioxide / Q000493:pharmacokinetics / Q000633:toxicity*; D009929:Organ Size / Q000187:drug effects; D011014:Pneumonia / Q000139:chemically induced; D011650:Pulmonary Alveoli / Q000473:pathology; D051381:Rats; D011919:Rats, Inbred Strains
D009585:Nitrogen Dioxide