Genotyping of bovine kappa-casein loci following DNA sequence amplification.
Caseins are a family of milk proteins that exist in several molecular forms (alpha s1, alpha s2, beta and kappa) with variant alleles of each. Kappa-caseins (kappa-CN) exist as A or B variants and are of particular importance to the quality of the milk. We report a rapid non-radioactive method for differentiating bovine k-CN alleles. Unlike direct milk protein analysis, this method can be utilized for genotyping sperm, or animals of either sex at an early age. A 350bp fragment of the bovine kappa-CN gene was amplified by the polymerase chain reaction (PCR) technique. The amplified product digested with HinfI endonuclease generated restriction fragment length polymorphisms (RFLP's) that allowed precise identification of kappa-CN AA, AB or BB genotypes. Hybridization with allele specific oligo-probes confirmed the RFLP identification of genotypes. The accurate and early identification of milk protein genotypes can have a direct impact on dairy cattle breeding strategies.
Fernando Medrano
J
J
Department of Animal Science, University of California, Davis 95616.
Aguilar-Cordova
E
E
D000483:Alleles; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002364:Caseins / Q000235:genetics*; D002417:Cattle / Q000235:genetics*; D005260:Female; D005784:Gene Amplification; D005796:Genes; D005838:Genotype; D008297:Male; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length
D002364:Caseins; D015345:Oligonucleotide Probes