Abnormalities of chromosomes 7 and 22 in human malignant pleural mesothelioma: correlation between Southern blot and cytogenetic analyses.
Southern blot hybridization studies were performed on 23 mesothelioma primary tumor specimens to detect chromosome 1-, 7-, and 22-specific numerical changes, gene amplifications, and gene rearrangements. The molecular findings were compared with previous cytogenetic findings. No gene rearrangements or amplifications were detected. A numerical abnormality of chromosome 7 was detected by Southern blot analysis in two cases in which no numerical abnormality had been detected by the previous chromosome study. A numerical aberration of chromosome 22 was detected in five cases, which was compatible with the cytogenetic finding of monosomy 22 in these cases. The Southern blot results for the copy numbers of chromosomes 7 and 22 were concordant with the cytogenetic findings in 65%-80% of the cases.
Department of Medical Genetics, University of Helsinki, Finland.
M
M
Tiainen
J
J
Kere
L
L
Tammilehto
K
K
Mattson
S
S
Knuutila
D015139:Blotting, Southern; D002869:Chromosome Aberrations; D025063:Chromosome Disorders; D002878:Chromosomes, Human, Pair 1; D002892:Chromosomes, Human, Pair 22; D002897:Chromosomes, Human, Pair 7; D005784:Gene Amplification; D015321:Gene Rearrangement; D006801:Humans; D007621:Karyotyping; D008654:Mesothelioma / Q000235:genetics*; D010997:Pleural Neoplasms / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length