In vitro differentiation of the human osteosarcoma cell lines, HOS and KHOS.
The osteogenic potential of the two human osteosarcoma cell lines HOS and KHOS; a cell line produced by the transformation of the HOS cells by the Kirsten murine sarcoma virus, was studied in vitro. HOS cells cultured more than 2 weeks formed nodules composed of two morphologically distinct layers, an epithelial-like surface cell layer and a collagen-rich inner cell layer. Alkaline phosphatase (ALPase) activity occurred in the plasma membrane of the surface cell layer, and calcified substances developing along collagen fibers were detected in the collagen-rich inner cell layer. The calcified substances were further examined by analytical electron microscopy and were shown to be hydroxyapatite crystals. In contrast, there was neither ALPase nor the deposition of a calcified substance in the KHOS cells.
Department of Molecular Cellular Oncology and Microbiology, Tokyo Medical and Dental University, Japan.
T
T
Ikeda
Y
Y
Futaesaku
N
N
Tsuchida
D000469:Alkaline Phosphatase / Q000378:metabolism; D002454:Cell Differentiation; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D003094:Collagen / Q000378:metabolism; D017886:Durapatite; D006801:Humans; D006882:Hydroxyapatites / Q000032:analysis; D012516:Osteosarcoma / Q000473:pathology* / Q000648:ultrastructure; D014407:Tumor Cells, Cultured
D006882:Hydroxyapatites; D003094:Collagen; D017886:Durapatite; D000469:Alkaline Phosphatase