Inhibition of phosphatidylserine synthesis by glutamate, acetylcholine, thapsigargin and ionophore A23187 in glioma C6 cells.
Phosphatidylserine synthesis was studied in glioma C6 cells with [14C]serine and in the presence or absence of agents which increase the level of [Ca2+]i. It was found that glutamate and acetylcholine inhibited this synthesis by up to 40%, whereas thapsigargin and the ionophore A23187 inhibited by up to 70%. The inhibitory effect of thapsigargin and the A23187 was observed in Ca(2+)-free medium. The data show that the inhibition of this synthesis is caused by the Ca(2+)-depletion from endoplasmic reticulum, suggesting that the synthesis of phosphatidylserine occurs on the luminal side of these structures and can be regulated by transmembrane signaling systems.
Czarny
M
M
Dept. of Cellular Biochemistry, Nencki Institute of Experimental Biology, Warsaw, Poland.
Sabała
P
P
Ucieklak
A
A
Kaczmarek
L
L
Barańska
J
J
D000109:Acetylcholine / Q000494:pharmacology*; D000818:Animals; D000001:Calcimycin / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism*; D002122:Calcium Chloride / Q000494:pharmacology; D000252:Calcium-Transporting ATPases / Q000037:antagonists & inhibitors; D004305:Dose-Response Relationship, Drug; D004492:Edetic Acid / Q000494:pharmacology; D016257:Fura-2; D005910:Glioma; D005971:Glutamates / Q000494:pharmacology*; D018698:Glutamic Acid; D007700:Kinetics; D010718:Phosphatidylserines / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D013729:Terpenes / Q000494:pharmacology*; D019284:Thapsigargin; D014407:Tumor Cells, Cultured
D005971:Glutamates; D010718:Phosphatidylserines; D013729:Terpenes; D000001:Calcimycin; D018698:Glutamic Acid; D019284:Thapsigargin; D004492:Edetic Acid; D000252:Calcium-Transporting ATPases; D002122:Calcium Chloride; D000109:Acetylcholine; D002118:Calcium; D016257:Fura-2