[Determination of colonization factor antigens CFA in diagnosis of enterotoxigenic strains of Escherichia coli].
This study was aimed at establishment whether preliminary determination of colonization factor antigens CFA may be useful in selection of potentially pathogenic strains of Escherichia coli with serological types belonging to ETEC and 750 isolates of E. coli from children with symptoms of diarrhoea. Enterotoxigenicity of strains was evaluated by suckling mice test and culture of Y1 cell tissue. Colonization factor antigens CFA were evaluated on the basis of slide agglutination and agar gel immunodiffusion with application diagnostic sera prepared for this study. Ability of enterotoxin production was found in 25% strains of E. coli with serological types belonging to ETEC. In 90% these strains were isolated from cases of epidemic diarrhoea. ETEC strains were found in 11% of hospitalized children and in 5% who were treated outside of hospital because of diarrhoea. MRHA adhesins occurred on 80% of ETEC strains were all diagnosed as CFA/I. CFA/II were not found and in only three strains non-fimbrial CFA/IV was present. Preliminary determination of CFA during selection of ETEC strains presents as a very sensitive method (97%) and is also highly specific (99%). Application of this method will result in significant increase of affectivity of biological tests directed toward determination of E. coli enterotoxigenicity.
Andziak
J
J
Zakład Bakteriologii PZH w Warszawie.
D000818:Animals; D001426:Bacterial Proteins / Q000032:analysis*; D002648:Child; D003967:Diarrhea / Q000382:microbiology*; D004768:Enterotoxins / Q000096:biosynthesis*; D004926:Escherichia coli / Q000145:classification / Q000276:immunology*; D036781:Fimbriae Proteins; D010861:Fimbriae, Bacterial; D006801:Humans; D051379:Mice; D012680:Sensitivity and Specificity; D012703:Serotyping; D013045:Species Specificity
D001426:Bacterial Proteins; D004768:Enterotoxins; C022924:colonization factor antigens; D036781:Fimbriae Proteins
Określanie czynników kolonizacyjnych CFA w diagnostyce enterotoksynogennych pałeczek Escherichia coli.