2-deoxy-D-glucose inhibits rejoining of radiation-induced DNA double-strand breaks in yeast.
Effects of 2-deoxy-D-glucose (2-DG) on radiation-induced DNA double-strand breaks (dsb) have been studied under non-growth conditions in a respiratory-deficient strain of the yeast Saccharomyces cerevisiae. Velocity sedimentation in neutral sucrose gradients was used to measure DNA dsb. Addition of 2-DG to the liquid-holding medium (67 mM phosphate buffer, pH 5, 30 degrees C) at an equimolar concentration with glucose (50 mM) reduced the rate and extent of dsb rejoining. The inhibition of rejoining mediated by 2-DG is reversible for the majority--but not all--of the radiation-induced dsb.
Institut für Biophysikalische Strahlenforschung, Gesellschaft für Strahlen-und Umweltforschung mbH, Frankfurt, Germany.
M
M
Frankenberg-Schwager
R
R
Harbich
D
D
Frankenberg
V
V
Jain
D004247:DNA / Q000528:radiation effects*; D004249:DNA Damage; D004260:DNA Repair / Q000187:drug effects*; D003847:Deoxyglucose / Q000494:pharmacology*; D012441:Saccharomyces cerevisiae / Q000235:genetics
D004247:DNA; D003847:Deoxyglucose