Nucleotide and deduced amino acid sequence of bovine adrenal medulla chromogranin B (secretogranin I).
1. A novel 1745-dalton pyroglutamyl peptide (BAM-1745)6 was recently isolated and characterized from bovine adrenal medulla chromaffin granules. Its amino acid sequence was found to be 93% identical to residues 580-593 of human chromogranin B (secretogranin I). 2. Based on this sequence a degenerate oligonucleotide probe was synthesized and used to identify a 2.4-kb bovine adrenal medulla chromogranin B cDNA. 3. The deduced polypeptide is 647 amino acids long and begins with a putative signal sequence of 20 residues as in the human, rat, and mouse proteins. Also conserved in the bovine protein is a tyrosine residue which may be sulfated, two N-terminal cysteines, and many paired basic amino acids which may serve as sites of posttranslational processing. The peptide BAM-1745 is flanked by paired basic amino acids and therefore is most likely a product of posttranslational processing. Bovine chromogranin B is 67, 58, and 58% identical to the human, rat, and mouse chromogranin B proteins, respectively. 4. The carboxyl terminus of bovine chromogranin B, including BAM-1745, was found to be the most conserved region of the polypeptide and may identify it as an important functional domain.
Vollum Institute for Advanced Biomedical Research, Oregon Health Sciences University, Portland 97021.
D K
DK
Grandy
R
R
Leduc
H
H
Makam
T
T
Flanagan
E J
EJ
Diliberto
O
O
Civelli
O H
OH
Viveros
D000313:Adrenal Medulla / Q000737:chemistry; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle / Q000235:genetics*; D053378:Chromogranin B; D002864:Chromogranins / Q000235:genetics* / Q000302:isolation & purification; D004247:DNA / Q000235:genetics; D006031:Glycosylation; D006801:Humans; D051379:Mice / Q000235:genetics; D008969:Molecular Sequence Data; D011061:Poly A / Q000235:genetics; D011499:Protein Processing, Post-Translational; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats / Q000235:genetics; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
C506134:CHGB protein, human; D053378:Chromogranin B; D002864:Chromogranins; D012333:RNA, Messenger; C506137:chromogranin B, mouse; C506140:chromogranin B, rat; D011061:Poly A; D004247:DNA