Delayed acute measles inclusion body encephalitis in a 9-year-old girl: ultrastructural, immunohistochemical, and in situ hybridization studies.
A 9-yr-old girl developed delayed acute measles inclusion body encephalitis, which was different from subacute sclerosing panencephalitis (SSPE) in clinical course. Measles virus was demonstrated by electron microscopy, immunohistochemistry, and in situ hybridization. Contrary to the most previous reports, matrix (M) protein was present in the brain, cerebrospinal fluid, and serum and was demonstrated by Western blot analysis and in situ hybridization. The hybridization was performed by a nonradioactive digoxigenin method.
Kim
T M
TM
Department of Pathology, Saint John Regional Hospital, Dalhousie Medical School, N.B. Canada.
Brown
H R
HR
Lee
S H
SH
Powell
C V
CV
Bethune
P
P
Goller
N L
NL
Tran
H T
HT
Mackay
J S
JS
D000956:Antigens, Viral / Q000302:isolation & purification; D001921:Brain / Q000382:microbiology / Q000473:pathology / Q000648:ultrastructure; D001923:Brain Chemistry; D002648:Child; D003586:Cytomegalovirus Infections / Q000150:complications / Q000209:etiology* / Q000473:pathology; D015342:DNA Probes; D003937:Diagnosis, Differential; D004076:Digoxigenin; D004673:Encephalomyelitis, Acute Disseminated / Q000150:complications / Q000209:etiology* / Q000473:pathology; D005260:Female; D006023:Glycoproteins / Q000097:blood / Q000134:cerebrospinal fluid; D006801:Humans; D007150:Immunohistochemistry; D008458:Measles Vaccine / Q000009:adverse effects*; D008459:Measles virus / Q000302:isolation & purification; D008854:Microscopy, Electron; D009693:Nucleic Acid Hybridization; D013344:Subacute Sclerosing Panencephalitis / Q000473:pathology
D000956:Antigens, Viral; D015342:DNA Probes; D006023:Glycoproteins; D008458:Measles Vaccine; C023587:protein M (glycoprotein); D004076:Digoxigenin