Hepatocyte response to continuous low dose-rate radiation in radioimmunotherapy assessed by micronucleus assay.
The response of hepatocytes to low dose-rate irradiation was examined in mice following the injection of radiolabelled monoclonal antibody. Mice were injected intravenously with an 131I-labelled monoclonal antibody 196-14 which recognizes CA125 antigen, and the effect of continuous low dose-rate irradiation on hepatocytes was assessed using the micronucleus assay. The frequency of micronuclei increased in a dose-dependent fashion, but it was lower than the frequency induced by conventional external X-rays which was determined immediately after the irradiation. A linear quadratic model (micronucleus frequency = aD+bD2+c) showed that the value of b decreased with low dose-rate irradiation from the radiolabelled antibody. It is concluded that the micronucleus assay is useful for the evaluation of the response of hepatocytes to irradiation in radioimmunotherapy.
Department of Nuclear Medicine, Kyoto University Hospital, Japan.
H
H
Sakahara
K
K
Ono
T
T
Saga
K
K
Akuta
K
K
Endo
J
J
Konishi
M
M
Abe
D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism / Q000627:therapeutic use; D005260:Female; D007074:Immunoglobulin G / Q000378:metabolism / Q000627:therapeutic use; D007457:Iodine Radioisotopes / Q000493:pharmacokinetics / Q000627:therapeutic use; D008099:Liver / Q000166:cytology / Q000378:metabolism / Q000528:radiation effects*; D051379:Mice; D008809:Mice, Inbred C3H; D015162:Micronucleus Tests; D016499:Radioimmunotherapy; D011879:Radiotherapy Dosage
D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D007457:Iodine Radioisotopes