Phylogenetic and epidemiological analysis of Neisseria meningitidis using DNA probes.
The genetic relationships between various serotypes and serogroups of meningococcal strains were investigated by restriction fragment-length polymorphism (RFLP) analysis using a number of random DNA probes and a probe containing a truncated copy of the meningococcal insertion sequence IS1106. The data were used to estimate genetic distance between all pairs of strains and to construct phylogenetic trees for meningococcal strains. B15:P1.16R strains isolated from cases of systemic meningococcal disease in two health districts with a high incidence of disease were clonal in contrast to similar strains from cases occurring in other parts of the UK. Strains from these areas, which contain a similar genomic deletion, were found to be derived from two distinct lineages within the B15:P1.16R phylogenetic group. RFLP data demonstrated that present serological typing systems for the meningococcus do not necessarily reflect true genetic relationships.
Molecular Microbiology Group, School of Biological Sciences, University of Surrey, Guildford, UK.
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McFadden
D015139:Blotting, Southern; D015342:DNA Probes; D004196:Disease Outbreaks / Q000706:statistics & numerical data; D005069:Evaluation Studies as Topic; D006801:Humans; D008589:Meningococcal Infections / Q000453:epidemiology / Q000235:genetics* / Q000382:microbiology; D009345:Neisseria meningitidis / Q000235:genetics*; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D012703:Serotyping; D006113:United Kingdom / Q000453:epidemiology
D015342:DNA Probes