Thy-1 inhibits mitogen-induced Ca2+ oscillation in ras-transformed mouse fibroblasts.
Cell surface glycoprotein Thy-1 functions as a transformation suppressor in v-ras-transformed NIH/3T3 cells [Sugimoto et al., (1991) Cancer Res. 51, 99-104.]. In order to understand the mechanism of action of Thy-1, we examined the effect of Thy-1 expression on mitogen-induced Ca2+ oscillation which was correlated with v-ras-transformation [Fu et al., (1991) FEBS Lett. 281, 263-266.]. Forced expression of Thy-1 in v-ras-transformed cells inhibited mitogen-induced Ca2+ oscillation. Although v-Ras-free, Thy-1-positive NIH/3T3 cells (major population) did not show Ca2+ oscillation, whereas in Thy-1-negative NIH/3T3 cells (less than 1% of the population) Ca2+ oscillation was observed. Finally, replacement of the carboxyl-half of Thy-1 with that of CD4 abolished the inhibitory effect of Thy-1. These results suggest that Thy-1 directly or indirectly participates in the negative regulation of Ca2+ response by inhibiting Ca2+ oscillation.
Sugimoto
Y
Y
Aging Process Research Laboratory (Frontier Research Program), Tsukuba Life Science Center, Japan.
Fu
T
T
Hirochika
R
R
Nakauchi
H
H
Ikawa
Y
Y
Nozawa
Y
Y
D016475:3T3 Cells; D000818:Animals; D000954:Antigens, Surface / Q000235:genetics / Q000502:physiology*; D001920:Bradykinin / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism*; D002461:Cell Line, Transformed; D005347:Fibroblasts / Q000502:physiology; D011905:Genes, ras; D007700:Kinetics; D008562:Membrane Glycoproteins / Q000235:genetics / Q000502:physiology*; D051379:Mice; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000502:physiology*; D015689:Oncogene Protein p21(ras) / Q000235:genetics / Q000378:metabolism; D018800:Thy-1 Antigens; D014162:Transfection
D000954:Antigens, Surface; D008562:Membrane Glycoproteins; D009419:Nerve Tissue Proteins; D018800:Thy-1 Antigens; D015689:Oncogene Protein p21(ras); D001920:Bradykinin; D002118:Calcium