Interleukin-6 is antiproliferative to a mouse hybridoma cell line and promotive for its antibody productivity.
Monoclonal antibody production by hybridoma cells at moderately slowed growth states would be favorable for commercial scale production since cells can devote their resources to performing the differentiated function, immunoglobulin production. We found that a purified recombinant human interleukin-6, which had been reported to support or stimulate proliferation of B cell hybridoma/plasmacytoma cells, suppressed growth of a hybridoma cell line in serum-free medium. In the presence of the interleukin, the growth-suppressed cells were viable for remarkably long periods in batch culture, and after removal of the interleukin from the culture medium, they started to proliferate at their normal growth rate. As the concentration of the interleukin increased in the culture, the growth rate decreased and the specific antibody productivity (antibody production rate per cell) increased to 5-fold of control at 10 U ml-1 (2 ng ml-1) of the interleukin.
Institute for Genetics, University of Cologne, Germany.
F
F
Makishima
S
S
Terada
T
T
Mikami
E
E
Suzuki
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D002455:Cell Division / Q000187:drug effects*; D016895:Culture Media, Serum-Free; D046508:Culture Techniques / Q000379:methods; D006131:Growth Inhibitors / Q000494:pharmacology*; D006825:Hybridomas / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D015850:Interleukin-6 / Q000494:pharmacology*; D007700:Kinetics; D051379:Mice; D011994:Recombinant Proteins / Q000494:pharmacology; D013997:Time Factors
D000911:Antibodies, Monoclonal; D016895:Culture Media, Serum-Free; D006131:Growth Inhibitors; D015850:Interleukin-6; D011994:Recombinant Proteins