Mapping genetic determinants for human immunodeficiency virus type 1 resistance to soluble CD4.
Neutralization of human immunodeficiency virus type 1 (HIV-1) infection with soluble CD4 (sCD4) can be achieved over a broad range of concentrations for different virus strains. Laboratory virus strains passaged in transformed T-cell lines are typically sensitive to sCD4 neutralization, whereas primary virus isolates require over 100-fold-higher sCD4 concentrations. Using recombinant viruses generated from a laboratory strain, HIV-1NL4-3, and a primary macrophagetropic strain, HIV-1JR-FL, we mapped a region of gp120 important for determining sensitivity to sCD4 neutralization. This same region has previously been defined as important for macrophage and transformed T-cell line tropism and includes the V3 neutralization domain but does not include regions of gp120 that have been shown to be most important for CD4 binding.
O'Brien
W A
WA
Department of Medicine, West Los Angeles Veteran's Administration Medical Center, California.
Chen
I S
IS
Ho
D D
DD
Daar
E S
ES
D000163:Acquired Immunodeficiency Syndrome / Q000235:genetics* / Q000517:prevention & control; D000937:Antigen-Antibody Reactions; D001665:Binding Sites; D015704:CD4 Antigens / Q000378:metabolism*; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D000939:Epitopes / Q000235:genetics* / Q000378:metabolism; D015699:HIV Envelope Protein gp120 / Q000235:genetics* / Q000378:metabolism; D015497:HIV-1 / Q000235:genetics* / Q000472:pathogenicity; D006801:Humans; D009928:Organ Specificity; D016133:Polymerase Chain Reaction; D011995:Recombination, Genetic
D015704:CD4 Antigens; D000939:Epitopes; D015699:HIV Envelope Protein gp120