Adhesion to thrombospondin by human embryonic fibroblasts is mediated by multiple receptors and includes a role for glycoprotein 88 (CD36).
Fetal embryonic fibroblasts attach and spread on thrombospondin (TSP). Adhesion is tight and focal adhesion plaques and "spots" are formed. We have investigated the receptors responsible for this adhesion. Unstimulated cells express the vitronectin receptor on their surface and this beta 3 integrin molecule contributes to adhesion. Another putative receptor for TSP, termed glycoprotein (GP) 88, which exists as a cytoplasmic pool in unstimulated cells becomes surface expressed when these cells are plated on TSP and localizes to areas of cell adhesion. Western blot analysis of cell lysate confirms GP88 as a TSP binding protein. Studies with fucoidan indicate that the heparan sulfate proteoglycan, known to function as a receptor for TSP, appears to contribute substantially to the TSP attachment of these cells and may be the receptor most important in the initial phases of TSP interaction.
Institute of Medical and Veterinary Science, Adelaide, Australia.
F C
FC
Stomski
J S
JS
Gani
R C
RC
Bates
G F
GF
Burns
D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000378:metabolism*; D015153:Blotting, Western; D018955:CD36 Antigens; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D011508:Chondroitin Sulfate Proteoglycans / Q000378:metabolism; D004622:Embryo, Mammalian; D005347:Fibroblasts / Q000378:metabolism*; D019812:Heparan Sulfate Proteoglycans; D006497:Heparan Sulfate / Q000378:metabolism; D006801:Humans; D016023:Integrins / Q000378:metabolism; D007700:Kinetics; D010980:Platelet Membrane Glycoproteins / Q000378:metabolism*; D011134:Polysaccharides / Q000494:pharmacology; D016024:Receptors, Cytoadhesin / Q000378:metabolism*; D011971:Receptors, Immunologic / Q000378:metabolism; D019030:Receptors, Vitronectin; D019699:Thrombospondins
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D018955:CD36 Antigens; D011508:Chondroitin Sulfate Proteoglycans; D019812:Heparan Sulfate Proteoglycans; D006497:Heparan Sulfate; D016023:Integrins; D010980:Platelet Membrane Glycoproteins; D011134:Polysaccharides; D016024:Receptors, Cytoadhesin; D011971:Receptors, Immunologic; D019030:Receptors, Vitronectin; D019699:Thrombospondins; C007789:fucoidan