Molecular characterization of Mexican stocks of Trypanosoma cruzi using total DNA.
Seventeen Mexican Trypanosoma cruzi stocks and five South American reference strains were analyzed by Hind III restriction fragment length polymorphisms associated with ribosomal RNA gene spacers and by HinfI digestion patterns of total DNA. Our findings demonstrate the occurrence of genetic heterogeneity within these stocks. Hierarchic and non-hierarchic clustering of these molecular characters allowed the formation of groups that correlate with the geographic origin of the stocks. The HinfI digestion pattern permitted the identification of DNA fragments from the kinetoplast maxi-circles, and therefore represents a simple and convenient method for conducting epidemiologic surveys in laboratories in developing countries.
Department of Molecular Biology, Universidad Nacional Autonoma de Mexico, Mexico City.
J E
JE
Zavala-Castro
O
O
Velasco-Castrejon
R
R
Hernandez
D000818:Animals; D015139:Blotting, Southern; D016000:Cluster Analysis; D004270:DNA, Circular / Q000032:analysis*; D018105:DNA, Kinetoplast; D016054:DNA, Protozoan / Q000032:analysis*; D015247:Deoxyribonuclease HindIII; D015252:Deoxyribonucleases, Type II Site-Specific; D004587:Electrophoresis, Agar Gel; D014644:Genetic Variation; D008800:Mexico; D012150:Polymorphism, Restriction Fragment Length; D016053:RNA, Protozoan / Q000032:analysis; D012335:RNA, Ribosomal / Q000032:analysis*; D015183:Restriction Mapping; D014349:Trypanosoma cruzi / Q000235:genetics*
D004270:DNA, Circular; D018105:DNA, Kinetoplast; D016054:DNA, Protozoan; D016053:RNA, Protozoan; D012335:RNA, Ribosomal; D015247:Deoxyribonuclease HindIII; D015252:Deoxyribonucleases, Type II Site-Specific; C032229:GANTC-specific type II deoxyribonucleases