Molecular design and catalytic function of a B12-enzyme mimetic.
Developing artificial holoenzymes requires attention to the molecular design, not only of the active site, but also of its microenvironment, which in the naturally occurring enzyme is provided by the apoprotein. Microenvironmental effects play a major role in determining the catalytic performance of an enzyme. Using the development of a B12-dependent holoenzyme mimetic as an example, the important factors and concepts underlying the approaches to developing an artificial holoenzyme are discussed.
Department of Organic Synthesis, Faculty of Engineering, Kyushu University, Fukuoka, Japan.
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Murakami
D000591:Amino Acid Isomerases / Q000737:chemistry; D001709:Biotechnology / Q000639:trends; D015195:Drug Design; D004798:Enzymes / Q000737:chemistry; D019751:Intramolecular Transferases; D008765:Methylmalonyl-CoA Mutase / Q000737:chemistry; D015394:Molecular Structure; D014805:Vitamin B 12 / Q000737:chemistry*
D004798:Enzymes; D000591:Amino Acid Isomerases; D019751:Intramolecular Transferases; C021570:methylaspartate mutase; D008765:Methylmalonyl-CoA Mutase; D014805:Vitamin B 12