The presence of free D-alanine, D-proline and D-serine in mice.
The presence of free D-alanine, D-proline and D-serine was demonstrated in mammalian tissues, using a mutant mouse strain lacking D-amino acid oxidase. In the experiment, free amino acids from the kidney and serum were derivatized with 1-fluoro-2,4-dinitrophenyl-5-L-alanine amide (FDAA) to diastereomers, separated by two-dimensional thin-layer chromatography (TLC), and analysed by reversed-phase high-performance liquid chromatography (HPLC) for the resolution of D- and L-isomers. D/L ratios of alanine, proline and serine were obtained based on the peak areas of HPLC.
Department of Biochemistry, Sapporo Medical College, Hokkaido, Japan.
Y
Y
Nagata
K
K
Yamamoto
T
T
Shimojo
R
R
Konno
Y
Y
Yasumura
T
T
Akino
D000409:Alanine / Q000032:analysis* / Q000097:blood; D000596:Amino Acids / Q000032:analysis*; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D003605:D-Amino-Acid Oxidase / Q000172:deficiency; D007668:Kidney / Q000737:chemistry; D051379:Mice; D008817:Mice, Mutant Strains; D011392:Proline / Q000032:analysis* / Q000097:blood; D012694:Serine / Q000032:analysis* / Q000097:blood; D013237:Stereoisomerism
D000596:Amino Acids; D012694:Serine; D011392:Proline; D003605:D-Amino-Acid Oxidase; D000409:Alanine