A molecular genetic linkage map of mouse chromosome 18 reveals extensive linkage conservation with human chromosomes 5 and 18.
An interspecific backcross between C57BL/6J and Mus spretus was used to generate a molecular genetic linkage map of mouse chromosome 18 that includes 23 molecular markers and spans approximately 86% of the estimated length of the chromosome. The Apc, Camk2a, D18Fcr1, D18Fcr2, D18Leh1, D18Leh2, Dcc, Emb-rs3, Fgfa, Fim-2/Csfmr, Gnal, Grl-1, Grp, Hk-1rs1, Ii, Kns, Lmnb, Mbp, Mcc, Mtv-38, Palb, Pdgfrb, and Tpl-2 genes were mapped relative to each other in one interspecific backcross. A second interspecific backcross and a centromere-specific DNA satellite probe were used to determine the distance of the most proximal chromosome 18 marker to the centromere. The interspecific map extends the known regions of linkage homology between mouse chromosome 18 and human chromosomes 5 and 18 and identifies a new homology segment with human chromosome 10p. It also provides molecular access to many regions of mouse chromosome 18 for the first time.
Mammalian Genetics Laboratory, ABL-Basic Research Program, NCI-Frederick Cancer Research and Development Center, Maryland 21702.
M J
MJ
Justice
D J
DJ
Gilbert
K W
KW
Kinzler
B
B
Vogelstein
A M
AM
Buchberg
J D
JD
Ceci
Y
Y
Matsuda
V M
VM
Chapman
C
C
Patriotis
A
A
Makris
D000818:Animals; D015139:Blotting, Southern; D002503:Centromere; D002874:Chromosome Mapping; D002887:Chromosomes, Human, Pair 18; D002895:Chromosomes, Human, Pair 5; D003433:Crosses, Genetic; D004247:DNA; D008040:Genetic Linkage; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D012150:Polymorphism, Restriction Fragment Length
D004247:DNA