Separation and purification of IgG1 and IgG2 from IgG-Cohn-II fraction of human plasma by pseudobioaffinity chromatography on histidyl-AH-sepharose.
L-histidine coupled to aminohexyl-sepharose (H-AH) has been used as an affinity sorbent to separate IgG from human plasma. Two subclasses IgG1 and IgG2 were specifically bound to histidyl-AH-sepharose at pH 7.4 and eluted using 0.2 M and 1M NaCl. The specificity of the two subclasses were determined by immunoelectrophoresis. Quantitative determination of IgG1, IgG2 was carried out using radial immunodiffusion technique.
el-Kak
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A
Département de Génie Biologique, Université de Technologie de Compiègne, France.
Vijayalakshmi
M A
MA
D002846:Chromatography, Affinity; D004587:Electrophoresis, Agar Gel; D006639:Histidine / Q000031:analogs & derivatives*; D006801:Humans; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D007074:Immunoglobulin G / Q000097:blood / Q000145:classification / Q000302:isolation & purification*; D007526:Isoelectric Point; D012680:Sensitivity and Specificity; D012685:Sepharose / Q000031:analogs & derivatives*
D007074:Immunoglobulin G; C080392:histidyl-AH-sepharose; D006639:Histidine; D012685:Sepharose