Induction of CD13 expression on fresh myeloid leukaemia: correlation of CD13 expression with aminopeptidase-N activity.
CD13 has proved to be a useful cell surface marker for depicting haematopoietic cells of the myeloid and monocytic lineages. Sequence data has shown CD13 to bear strong homology to Aminopeptidase-N. Expression of this antigen on myeloid leukaemic cells is, however, variable. We have looked at the effects of a phorbol ester PMA and a haematopoietic growth factor GM-CSF on the expression of CD13 displayed by a selected group of myeloid leukaemias at presentation and by normal peripheral blood granulocytes. We have found that PMA but not GM-CSF was able to stimulate CD13 expression on fresh myeloid leukaemic cells but granulocytes were stimulated by either PMA or GM-CSF. In addition, we have correlated CD13 expression with Aminopeptidase-N enzyme activity on fresh myeloid leukaemic cells. These results suggest that CD13 is functionally active on leukaemic cells and normal granulocytes.
Razak
K
K
Department of Haematology, London Hospital Medical College, Whitechapel, U.K.
Newland
A C
AC
D000626:Aminopeptidases / Q000378:metabolism*; D015703:Antigens, CD / Q000378:metabolism*; D015214:Antigens, Differentiation, Myelomonocytic / Q000378:metabolism*; D000951:Antigens, Neoplasm / Q000378:metabolism*; D018826:CD13 Antigens; D005434:Flow Cytometry; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D006098:Granulocytes / Q000276:immunology; D006801:Humans; D016130:Immunophenotyping; D007951:Leukemia, Myeloid / Q000201:enzymology / Q000276:immunology* / Q000473:pathology; D012044:Regression Analysis; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000201:enzymology / Q000276:immunology
D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D000951:Antigens, Neoplasm; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D000626:Aminopeptidases; D018826:CD13 Antigens; D013755:Tetradecanoylphorbol Acetate