Nitric oxide-releasing compounds inhibit Dictyostelium discoideum aggregation without altering cGMP production.
The effects of nitric oxide-releasing compounds on Dictyostelium discoideum cell development and guanylyl cyclase activity were studied. The addition of SNP (sodium nitroprusside) or SIN-1 (3-morpholino-syndnonimine) to starved cells inhibited their differentiation and aggregation in a concentration-dependent manner. In contrast to mammalian systems, SNP did not significantly affect guanylyl cyclase activity in cell lysates of D. discoideum, nor did it stimulate cGMP production in intact cells. The results suggest that the inhibitory effects of NO on D. discoideum cell aggregation are through a mechanism independent of an effect on guanylyl cyclase activity.
Department of Biochemistry and Molecular Biology, St. Louis University School of Medicine, MO.
Y
Y
Tao
A
A
Howlett
C
C
Klein
D000818:Animals; D000959:Antihypertensive Agents / Q000494:pharmacology; D000242:Cyclic AMP / Q000494:pharmacology; D006152:Cyclic GMP / Q000378:metabolism*; D004023:Dictyostelium / Q000187:drug effects* / Q000201:enzymology; D006162:Guanylate Cyclase / Q000378:metabolism; D008981:Molsidomine / Q000031:analogs & derivatives* / Q000494:pharmacology; D009024:Morphogenesis / Q000187:drug effects*; D009569:Nitric Oxide / Q000378:metabolism*; D009599:Nitroprusside / Q000494:pharmacology*; D014665:Vasodilator Agents / Q000494:pharmacology
D000959:Antihypertensive Agents; D014665:Vasodilator Agents; D009599:Nitroprusside; D009569:Nitric Oxide; C002385:linsidomine; D008981:Molsidomine; D000242:Cyclic AMP; D006162:Guanylate Cyclase; D006152:Cyclic GMP