Amplification and direct sequencing of a cDNA encoding human cytosolic 3-hydroxy-3-methylglutaryl-coenzyme A synthase.
Cytosolic 3-hydroxy-3-methylglutaryl CoA (HMG-CoA) synthase (E.C. 4.1.3.5) is a highly regulated enzyme involved in isoprenoid biosynthesis and therefore a potential target for cholesterol-lowering drugs. Up to now, primary structure data have only been available for chicken, rat and hamster HMG-CoA synthase. Using in vitro amplification and direct sequencing, we have determined the nucleotide sequence of the coding region of the human cytosolic 3-hydroxy-3-methylglutaryl CoA synthase cDNA.
Russ
A P
AP
Gustav Embden-Zentrum der biologischen Chemie, J.W. Goethe-Universität, Frankfurt, Germany.
Ruzicka
V
V
Maerz
W
W
Appelhans
H
H
Gross
W
W
D000595:Amino Acid Sequence; D001483:Base Sequence; D004247:DNA; D006801:Humans; D006904:Hydroxymethylglutaryl-CoA Synthase / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction
D004247:DNA; D006904:Hydroxymethylglutaryl-CoA Synthase