Industrial production of monoclonal antibodies and therapeutic proteins by dialysis fermentation.
A novel and powerful fermentation method is reported for the large-scale growth of mammalian cells and their secreted products. The system described illustrates many of the advantages of conventional batch fermentation processes but in addition has been shown to yield cell densities in excess of 1 x 10(7) cells/ml with concomitant increase in product concentration.
Comer
M J
MJ
Boehringer Mannheim GmbH, Research Centre Penzberg, FRG.
Kearns
M J
MJ
Wahl
J
J
Munster
M
M
Lorenz
T
T
Szperalski
B
B
Koch
S
S
Behrendt
U
U
Brunner
H
H
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D001688:Biological Products / Q000096:biosynthesis*; D002478:Cells, Cultured / Q000378:metabolism*; D003956:Dialysis; D005285:Fermentation; D008722:Methods; D051379:Mice
D000911:Antibodies, Monoclonal; D001688:Biological Products