An improved method for rapid isolation of plasmid DNA from wild-type gram-negative bacteria for plasmid restriction profile analysis.
An improved method for the isolation of large and small plasmids from wild-type Gram-negative bacteria has been developed. The protocol combines the lysis and purification procedures of two popular plasmid isolation methods, and produces DNA sufficiently pure for restriction enzyme digestion in less than three hours.
Olsen
J E
JE
Department of Hygiene and Microbiology, Royal Veterinary and Agricultural University, Frederiksberg C., Denmark.
D004269:DNA, Bacterial / Q000032:analysis / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel; D006090:Gram-Negative Bacteria / Q000235:genetics*; D010957:Plasmids; D015183:Restriction Mapping
D004269:DNA, Bacterial