Expression of proliferating cell nuclear antigen during the cell cycle of human diploid fibroblasts.
Proliferating cell nuclear antigen mRNA levels were determined in human diploid fibroblasts as they progressed through the cell cycle. PCNA message levels were low at G0, gradually increased following entrance into G1, peaked at G1/S, and declined during S phase. PCNA mRNA was determined to have a half life of 12 hours when cells were blocked at the G1/S interface. PCNA protein levels increased two- to three-fold as cells moved from G0 to S phase.
Samuel Roberts Noble Foundation, Inc., Ardmore, Oklahoma 73402.
C A
CA
Stewart
R T
RT
Dell'Orco
D002478:Cells, Cultured; D005347:Fibroblasts; D006207:Half-Life; D006801:Humans; D007399:Interphase; D009687:Nuclear Proteins / Q000096:biosynthesis* / Q000235:genetics; D018809:Proliferating Cell Nuclear Antigen; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D016196:S Phase
D009687:Nuclear Proteins; D018809:Proliferating Cell Nuclear Antigen; D012333:RNA, Messenger