Comparative behaviour of L-929 fibroblastic and human endothelial cells onto crosslinked protein substrates.
Studies were carried out to compare the behaviour of human umbilical vein endothelial cells (HUVEC) and L-929 fibroblastic cells towards proteins crosslinked by glutaraldehyde (GTA) or carbodiimide (CDI) proposed for coating of vascular prostheses. CDI crosslinking of bovine serum albumin used alone, or mixed with gelatin, allowed higher rates of cell growth and DNA synthesis than GTA crosslinking independent of cells. Assessment of the plating efficiency revealed a similar behaviour of both cells towards membranes and reference plastic surface in terms of percentages of bound cells. HUVEC proliferation onto CDI crosslinked gelatin and/or albumin membranes did not differ significantly whereas the growth of L-929 was enhanced onto gelatin albumin membranes in comparison with both gelatin membranes and the reference surface. The analysis of DNA synthesis corroborated the results of the growth curves and elicited a delay of the growth phases in HUVEC cultured onto CDI crosslinked membranes, unlike the L-929 fibroblast.
Warocquier-Clérout
R
R
Départment de Génie Biologique, Université de Technologie de Compiègne, France.
Lee
Y S
YS
Penhoat
J
J
Sigot-Luizard
M F
MF
D000418:Albumins; D000818:Animals; D002234:Carbodiimides; D002448:Cell Adhesion; D002455:Cell Division; D002460:Cell Line / Q000166:cytology*; D002478:Cells, Cultured / Q000166:cytology*; D003432:Cross-Linking Reagents; D004261:DNA Replication; D004730:Endothelium, Vascular / Q000166:cytology*; D005347:Fibroblasts / Q000166:cytology*; D005780:Gelatin; D005976:Glutaral; D006801:Humans; D008566:Membranes; D008855:Microscopy, Electron, Scanning
D000418:Albumins; D002234:Carbodiimides; D003432:Cross-Linking Reagents; D005780:Gelatin; D005976:Glutaral