CP-82,996, a novel diglycoside polyether antibiotic related to monensin and produced by Actinomadura sp.
A new polyether antibiotic CP-82,996 (C50H86O16) was isolated by solvent extraction from the fermentation broth of Actinomadura sp. (ATCC 53764). Following purification by silica gel column chromatography and crystallization, the structure of CP-82,996 was determined by a single crystal X-ray analysis. The structure is closely related to monensin, but is unique in that it contains two sugar groups, whereas monensin has none. The 1H and 13C NMR chemical shifts and assignments for CP-82,996 were elucidated, and they were compared with those determined previously for monensin. CP-82,996 is active against certain Gram-positive bacteria, and is a very potent anticoccidial agent. It effectively controlled chicken coccidiosis caused by several Eimeria species at 5-10 ppm in feed, and is 10-20 times more potent than monensin.
Central Research, Pfizer Inc., Groton, CT 06340.
J P
JP
Dirlam
A M
AM
Belton
J
J
Bordner
W P
WP
Cullen
L H
LH
Huang
Y
Y
Kojima
H
H
Maeda
S
S
Nishiyama
J R
JR
Oscarson
A P
AP
Ricketts
D000818:Animals; D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use; D001419:Bacteria / Q000187:drug effects*; D002645:Chickens; D003048:Coccidiosis / Q000188:drug therapy / Q000662:veterinary*; D005285:Fermentation; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008855:Microscopy, Electron, Scanning; D015394:Molecular Structure; D008985:Monensin / Q000031:analogs & derivatives / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use; D009618:Nocardiaceae / Q000145:classification / Q000378:metabolism* / Q000648:ultrastructure; D011201:Poultry Diseases / Q000188:drug therapy*; D014961:X-Ray Diffraction
D000900:Anti-Bacterial Agents; C068005:CP 82996; D008985:Monensin