Polymorphism of class I alcohol dehydrogenase in French, Vietnamese and Niger populations: genotyping by PCR amplification and RFLP analysis on dried blood spots.
The polymorphism of human alcohol dehydrogenase (ADH) can contribute to the explanation of the important ethnic differences towards alcohol metabolism. Its assessment at the genomic DNA level with a procedure, excluding labelled probes, consisting of PCR (Polymerase chain reaction) amplification on dried blood spots and analysis of allele-specific RFLP (Restriction fragment length polymorphism) profiles, is well adapted to extensive studies in population samples. It can emphasize the importance of ADH as a genetic marker of population. Three ethnic groups (French Caucasians, Vietnamese Orientals, Black Africans from Niger) were studied. ADH2 and ADH3 genotypes were in equilibrium according to the Hardy-Weinberg law. Important differences were noted in the distribution of ADH2 and ADH3 alleles.
Département de Biochimie Médicale et Biologie Moléculaire, Université de Bordeaux II, France.
A
A
Iron
A
A
Groppi
B
B
Fleury
J
J
Begueret
A
A
Cassaigne
P
P
Couzigou
D000426:Alcohol Dehydrogenase / Q000235:genetics* / Q000378:metabolism; D000438:Alcohols / Q000378:metabolism; D000483:Alleles; D001769:Blood; D005602:France / Q000208:ethnology; D005784:Gene Amplification / Q000235:genetics; D005838:Genotype; D006801:Humans; D009548:Niger / Q000208:ethnology; D021141:Nucleic Acid Amplification Techniques; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D044469:Racial Groups; D014744:Vietnam / Q000208:ethnology
D000438:Alcohols; D000426:Alcohol Dehydrogenase