Microtiter assay for measurement of factor XIII activity in plasma.
A sensitive, reproducible and rapid quantitative microtiter assay for factor XIII activity is described. The assay is based on incorporation of a soluble amine substrate into casein attached to polystyrol microtiter plates and detection of bound substrate with a monoclonal antibody-enzyme conjugate. Defibrination of plasma samples is not required. The assay shows strong correlation with the immunologic assay for factor XIII catalytic subunit a (r = 0.94), the factor XIII dansylcadaverine assay (r = 0.83), and the factor XIII clot solubility test. Normal values detected in healthy blood donors were in the range of 74% to 117%, with a mean value of 96.9% and a median value of 97.8%; pooled normal plasma was used as a reference. Since the assay is performed in a single microtiter plate and requires few reagents and short incubation periods, it can be easily adapted for clinical use.
University of Heidelberg, Faculty of Clinical Medicine Mannheim, First Dept. of Medicine, Germany.
C E
CE
Dempfle
J
J
Harenberg
K
K
Hochreuter
D L
DL
Heene
D002364:Caseins; D005176:Factor XIII / Q000032:analysis*; D005340:Fibrinogen; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D011503:Transglutaminases / Q000032:analysis
D002364:Caseins; D005340:Fibrinogen; D005176:Factor XIII; D011503:Transglutaminases