N-laurylbiotinamide as affinity surfactant.
N-laurylbiotinamide (NLB), which retains strong affinity for the protein avidin, was synthesized from biotin and N-laurylamine via the biotin ester of N-hydroxysuccinimide and characterized by NMR. When the synthesized NLB was used as a cosurfactant with AOT to form a reverse micellar system in isooctane, it was found to extend the pH range over which avidin can be transferred from a continuous aqueous solution to the reverse-micellar phase. This behavior is similar to that already reported for a different affinity surfactant, n-octyl beta-D-glucopyranoside.
Department of Chemical Engineering, University of Connecticut, Storrs 06269.
R W
RW
Coughlin
J B
JB
Baclaski
D001360:Avidin / Q000737:chemistry / Q000302:isolation & purification; D001710:Biotin / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004143:Dioctyl Sulfosuccinic Acid; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy; D008823:Micelles; D009795:Octanes; D012996:Solutions; D013501:Surface-Active Agents; D014867:Water
D008823:Micelles; D009795:Octanes; D012996:Solutions; D013501:Surface-Active Agents; D014867:Water; D004143:Dioctyl Sulfosuccinic Acid; C066881:N-laurylbiotinamide; D001360:Avidin; D001710:Biotin; C045798:2,2,4-trimethylpentane