Preparation of polyclonal antibodies to an anti-tumor (1----3)-beta-D-glucan, schizophyllan.
Antibodies against Schizophyllan (SPG) or SPG conjugated with bovine serum albumin (BSA) were raised in rabbits by multiple subcutaneous immunization. The titer of SPG-reactive antibodies in the antiserum to SPG-BSA conjugate was significantly higher than in the antiserum to SPG as assessed by enzyme-linked immunosorbent assay (ELISA). The SPG-reactive antibodies purified by affinity chromatography using a Protein A-Sepharose CL-4B column interacted with the SPG-BSA conjugate spotted on a nitrocellulose membrane filter in a dose-dependent manner, but the anti-SPG antibodies did not interact with BSA. Immunocytochemical staining has also shown that the anti-SPG antibodies reacted with SPG taken up by mouse peritoneal macrophages.
Research Laboratory, Taito Co., Ltd., Kobe, Japan.
K
K
Tabata
W
W
Itoh
A
A
Hirata
I
I
Sugawara
S
S
Mori
D000818:Animals; D000906:Antibodies / Q000276:immunology*; D000917:Antibody Formation; D000918:Antibody Specificity; D000970:Antineoplastic Agents / Q000276:immunology*; D005936:Glucans / Q000276:immunology*; D007114:Immunization; D015151:Immunoblotting; D007150:Immunohistochemistry / Q000379:methods; D008264:Macrophages / Q000276:immunology / Q000378:metabolism; D008297:Male; D051379:Mice; D010529:Peritoneal Cavity / Q000166:cytology; D011817:Rabbits; D012710:Serum Albumin, Bovine / Q000276:immunology / Q000378:metabolism; D012566:Sizofiran / Q000276:immunology* / Q000378:metabolism; D047071:beta-Glucans
D000906:Antibodies; D000970:Antineoplastic Agents; D005936:Glucans; D047071:beta-Glucans; D012710:Serum Albumin, Bovine; D012566:Sizofiran; C033363:beta-1,3-glucan