Establishment of cytotoxic CD4+ T cell clones from cancer patients treated by local immunotherapy.
We have previously reported that the antitumor effect of OK-432, a Streptococcal preparation, is markedly augmented when injected intratumorally together with fibrinogen (Cancer, 69: 636-642, 1992). In order to elucidate the mechanism of the antitumor effects, we established T cell clones from regional lymph nodes of colorectal cancer patients who received this local immunotherapy. By culture of lymph node lymphocytes, in the presence of IL-2 and OK-432, 4 clones of T cells were established from 4 patients treated by local immunotherapy. These clones had a helper T cell phenotype (CD3+, CD4+, CD8-, CD56-, WT31+) and were successfully maintained for several months. The cells strongly expressed CD25 when stimulated with OK-432 and exhibited a high level of cytotoxic activity in part explained by the increased expression of ICAM-1 and LFA-1, and the release of TNF beta. These results suggest that the CD4+ T cells play a role in the antitumor mechanism of local immunotherapy.
Nagaoka
H
H
Second Department of Surgery, Osaka University Medical School, Japan.
Monden
T
T
Sakita
I
I
Katsumoto
Y
Y
Wakasugi
T
T
Kawasaki
Y
Y
Tomita
N
N
Takeda
T
T
Yagyu
T
T
Morimoto
H
H
D015496:CD4-Positive T-Lymphocytes / Q000166:cytology* / Q000187:drug effects / Q000276:immunology; D015815:Cell Adhesion Molecules / Q000502:physiology; D002999:Clone Cells; D015179:Colorectal Neoplasms / Q000276:immunology* / Q000628:therapy; D005434:Flow Cytometry; D006801:Humans; D007150:Immunohistochemistry; D007167:Immunotherapy; D018799:Intercellular Adhesion Molecule-1; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology; D008233:Lymphotoxin-alpha / Q000502:physiology; D010641:Phenotype; D010844:Picibanil / Q000627:therapeutic use; D013268:Stimulation, Chemical; D013602:T-Lymphocytes, Cytotoxic / Q000166:cytology* / Q000187:drug effects / Q000276:immunology
D015815:Cell Adhesion Molecules; D008233:Lymphotoxin-alpha; D018799:Intercellular Adhesion Molecule-1; D010844:Picibanil