DNA stabilization and amplification from museum collections of extracts originally intended for allozyme analysis.
Protein extracts originally prepared for isozyme electrophoresis two decades ago contain surviving DNA sequences susceptible to amplification by the polymerase chain reaction (PCR). Amplification was also possible after stabilization of such extracts on filter paper and immersion under mineral oil without refrigeration.
Museum of Vertebrate Zoology, University of California, Berkeley 94720.
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D000818:Animals; D001483:Base Sequence; D003573:Cytochrome b Group / Q000235:genetics; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification*; D017931:DNA Primers / Q000235:genetics; D004355:Drug Stability; D017753:Ecosystem; D004798:Enzymes / Q000235:genetics / Q000302:isolation & purification; D005784:Gene Amplification; D008899:Mineral Oil; D008969:Molecular Sequence Data; D009144:Museums; D016133:Polymerase Chain Reaction / Q000379:methods*; D013997:Time Factors; D014562:Caudata / Q000235:genetics
D003573:Cytochrome b Group; D017931:DNA Primers; D004798:Enzymes; D008899:Mineral Oil; D004247:DNA