Bone marrow processing with the Fresenius AS 104: initial results.
Prior to purging, cryopreservation, or ABO-incompatible bone marrow (BM) transplantation, we have concentrated 23 BM harvests using a modification of the "grancollect-protocol" and the recently available bone marrow stem cell (BMSC) protocol of the Fresenius AS 104 cell separator with the P1-Y set. Within 40-70 minutes, the initial marrow volume of 922 ml (+/- 408 ml) was processed two to three times. A mean of 59% (+/- 20%) of the initial mononuclear cells was recovered in a mean volume of 119 ml (+/- 31 ml). The recovery of clonogenic cells, measured by CFU-GM assays, was 98% (+/- 80%). Red blood cells in the BM concentrates were reduced to 8% (+/- 4%) of the initial number. The procedure was efficient and yielded a BM cell fraction suitable for purging, cryopreservation, and transplantation. All transplanted patients showed fast and sustained engraftments after autologous or allogeneic BM transplantation.
Zingsem
J
J
Abt. Innere Medizin und Poliklinik m.S. Hämatologie und Onkologie, Universitätsklinikum Rudolf Virchow, Freie Universität Berlin, FRG.
Zeiler
T
T
Zimmermann
R
R
Weisbach
V
V
Mitschulat
H
H
Siegert
W
W
Eckstein
R
R
D001854:Bone Marrow Cells; D002469:Cell Separation / Q000295:instrumentation*; D004906:Erythrocyte Count; D005069:Evaluation Studies as Topic; D006801:Humans; D007938:Leukemia / Q000473:pathology; D007958:Leukocyte Count; D008223:Lymphoma / Q000473:pathology; D012189:Retrospective Studies