Application and assessment of cloacin typing of Enterobacter cloacae.
Three methods, O-serotyping, phage typing and susceptibility to bacteriocins, were used to type 357 clinical isolates of Enterobacter cloacae cultured from 219 patients. One hundred and sixty isolates were typed by serology and phage typing. When these two methods were used, primary classification of isolates was based on serology (65.7% typable) and phage typing for further subdivision (94.1% typable). When all the isolates were typed by cloacin susceptibility, 81.5% of them were typable. Maximum discrimination between cultures was achieved when the three methods were used together; no single method was sufficiently discriminatory. There was a close parallel between serotyping and bacteriocin lysis pattern. The latter was easy to perform and the results were achieved within 48 h. By applying this typing system two episodes of cross-infection were identified in a haematology/oncology unit and intensive care unit.
Department of Clinical Microbiology, Trinity College, Dublin, Ireland.
M A
MA
Daw
G D
GD
Corcoran
F R
FR
Falkiner
C T
CT
Keane
D001434:Bacteriophage Typing; D002989:Cloacin / Q000302:isolation & purification*; D016972:Enterobacter cloacae / Q000145:classification* / Q000254:growth & development; D005243:Feces / Q000382:microbiology; D006801:Humans; D009666:Nose / Q000382:microbiology; D019081:O Antigens; D010614:Pharynx / Q000382:microbiology; D011135:Polysaccharides, Bacterial / Q000302:isolation & purification; D016036:Seroepidemiologic Studies; D012703:Serotyping / Q000379:methods*
D019081:O Antigens; D011135:Polysaccharides, Bacterial; D002989:Cloacin