Sequence and expression of rat ICAM-1.
We have isolated cDNA clones-coding for rat intercellular adhesion molecule-1 (RICAM-1) from a cDNA library constructed from rat Ax cells stimulated with IL-1 beta using the mouse ICAM-1 cDNA as a hybridization probe. The RICAM-1 sequence shows 79.1% homology with mouse ICAM-1 and 55.6% homology with human ICAM-1 at the nucleic acid level. In order to examine the expression of RICAM-1 on Chinese hamster ovary (CHO) cells, we constructed the vector, pSV-RICAM1-neo, containing the SV40 promoter. Flowcytometric analysis showed that CHO-K1 cells transfected with pSV-RICAM1-neo expressed high amounts of RICAM-1 on their surfaces.
Kita
Y
Y
Molecular Biology Research Laboratory, Tokyo R and D Center, Daiichi Pharmaceutical Co., Japan.
Takashi
T
T
Iigo
Y
Y
Tamatani
T
T
Miyasaka
M
M
Horiuchi
T
T
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015815:Cell Adhesion Molecules / Q000235:genetics* / Q000378:metabolism; D004247:DNA / Q000302:isolation & purification*; D018799:Intercellular Adhesion Molecule-1; D008969:Molecular Sequence Data; D051381:Rats; D016415:Sequence Alignment
D015815:Cell Adhesion Molecules; D018799:Intercellular Adhesion Molecule-1; D004247:DNA