Uptake, metabolism, and release of N-[3H]acetylaspartylglutamate by the avian retina.
N-Acetylaspartylglutamate (NAAG) is a nervous system-specific dipeptide that is released from retinal neurons on depolarization. In the present study, extracellular metabolism, uptake, and release of [3H]NAAG were examined in the chick retina. After in vitro incubation with NAAG radiolabeled in the glutamate moiety, [3H]glutamate and [3H]NAAG increased in retinal cells through time- and temperature-dependent processes, which were reduced in the absence of extracellular sodium. Coincubation of cells with [3H]NAAG and aspartylglutamate or phosphate resulted in the decreased extracellular appearance of [3H]glutamate, produced by hydrolysis of radiolabeled NAAG, and a consequent increased availability of [3H]NAAG for transport into the retinal cells. When this tissue was incubated with radiolabeled NAAG, glutamate, glutamine, or aspartate under similar conditions, only [3H]NAAG served as a significant source for the appearance of intracellular [3H]NAAG. These data support the conclusion that [3H]NAAG can be transported into retinal cells, whereas [3H]glutamate transport is the predominant process after release of this amino acid from NAAG by extracellular peptidase activities. After uptake, [3H]NAAG entered a cellular pool, from which the peptide was secreted under depolarizing conditions and in a calcium-dependent manner.
Department of Biology, Georgetown University, Washington, DC 20057.
L C
LC
Williamson
J H
JH
Neale
D000818:Animals; D001224:Aspartic Acid / Q000378:metabolism / Q000493:pharmacokinetics; D001692:Biological Transport / Q000502:physiology; D002645:Chickens; D004151:Dipeptides / Q000378:metabolism* / Q000493:pharmacokinetics*; D005971:Glutamates / Q000378:metabolism / Q000493:pharmacokinetics; D018698:Glutamic Acid; D005973:Glutamine / Q000378:metabolism / Q000493:pharmacokinetics; D006634:Histamine H1 Antagonists / Q000378:metabolism* / Q000493:pharmacokinetics*; D012160:Retina / Q000378:metabolism* / Q000502:physiology; D013997:Time Factors; D014316:Tritium
D004151:Dipeptides; D005971:Glutamates; D006634:Histamine H1 Antagonists; D005973:Glutamine; D014316:Tritium; C027172:isospaglumic acid; D001224:Aspartic Acid; D018698:Glutamic Acid