Simultaneous diagnosis of coagulation factor XIIA (F13A) and plasminogen (PLG) phenotypes by polyacrylamide gel isoelectric focusing.
In this paper, we report a simple rapid method for simultaneous determination of Coagulation Factor XIIIA (F13A) and plasminogen (PLG) phenotypes by PAGIF with a nominal pH range of 3.5 to 10, followed by immunofixation and silver stain. Critical considerations concerning the conditions of molecular separation and detection strategies are also presented.
Caeiro
J L
JL
Departamento de Antropoloxía, Facultade de Bioloxía, Universidade de Santiago de Compostela, Galicia, Spain.
Llano
C
C
Parra
E
E
D005819:Genetic Markers; D006579:Heterozygote; D006720:Homozygote; D006801:Humans; D007525:Isoelectric Focusing; D010641:Phenotype; D010958:Plasminogen / Q000032:analysis* / Q000235:genetics; D015203:Reproducibility of Results; D011503:Transglutaminases / Q000032:analysis* / Q000235:genetics
D005819:Genetic Markers; D010958:Plasminogen; D011503:Transglutaminases