The proliferative kinetics of somatostatin-producing cells in the rat antral mucosa after truncal vagotomy.
The kinetics of somatostatin-producing cells (D-cells) in the rat antral mucosa after truncal vagotomy were studied using double immunostaining for bromodeoxyuridine (BrdU) and somatostatin. Both the concentration of somatostatin and D-cell density in the antral mucosa demonstrated a significant increase on the 3rd day after truncal vagotomy. With the single labeling of BrdU, a few D-cells showed positive immunostaining for BrdU throughout the experimental period in both vagotomized and sham operated rats. With cumulative labeling, the BrdU labeled cells demonstrated a linear increase in an identical number for each experimental time-point in both groups. The labeling index of BrdU in the D-cells increased significantly, beginning on the 3rd day and attaining a maximum level of 41.8% on the 10th day, in the vagotomized group after cumulative labeling. In this group, however, the density of D-cells with no immunoreactive BrdU also increased quickly on the 3rd day with cumulative labeling. The present study indicates that the most important factor involving D-cell hyperplasia observed after truncal vagotomy is the activation of pre-existing D-cells to synthesize and release hormones, together with the rapid replication of progenitor cells and their maturation to D-cells.
Shimoda
H
H
Second Department of Surgery, Medical College of Oita, Japan.
Yokoyama
S
S
Nakayama
I
I
Hadama
T
T
Uchida
Y
Y
D000818:Animals; D001973:Bromodeoxyuridine / Q000032:analysis; D002452:Cell Count; D002455:Cell Division; D005753:Gastric Mucosa / Q000166:cytology* / Q000378:metabolism; D008297:Male; D011184:Postoperative Period; D011706:Pyloric Antrum / Q000166:cytology* / Q000378:metabolism; D011863:Radioimmunoassay; D051381:Rats; D017208:Rats, Wistar; D013004:Somatostatin / Q000032:analysis / Q000096:biosynthesis*; D015167:Vagotomy, Truncal
D013004:Somatostatin; D001973:Bromodeoxyuridine