Molecular cloning and expression of chicken C-terminal Src kinase: lack of stable association with c-Src protein.
Cloning and sequencing of chicken C-terminal Src kinase (CSK), a tyrosine kinase that phosphorylates the regulatory C-terminal tyrosine residue present on cytoplasmic tyrosine kinases of the Src family, demonstrated a high degree of interspecies conservation as well as src homology 2 and 3 domains N-terminal to the kinase domain. The lack of autophosphorylation sites distinguishes CSK from other tyrosine kinases. CSK is unique and does not belong to a gene family, suggesting that it may phosphorylate other members of the Src family of tyrosine kinases in addition to c-Src. Since complex formation between c-Src and CSK seemed a likely regulatory step in the control of c-Src kinase activity, such an association was investigated by immunoprecipitation and Western blotting as well as intracellular localization studies. Although some portions of CSK were found in a membrane fraction, no complex formation between CSK and c-Src was observed, suggesting that the src homology 2 domain of CSK does not play a role in the direct interaction of c-Src.
Laboratory of Molecular Oncology, Rockefeller University, New York, NY 10021-6399.
H
H
Sabe
B
B
Knudsen
M
M
Okada
S
S
Nada
H
H
Nakagawa
H
H
Hanafusa
D000595:Amino Acid Sequence; D000818:Animals; D016367:Baculoviridae / Q000235:genetics; D001483:Base Sequence; D015139:Blotting, Southern; D000081247:CSK Tyrosine-Protein Kinase; D002460:Cell Line; D002478:Cells, Cultured; D002642:Chick Embryo; D002645:Chickens; D003001:Cloning, Molecular / Q000379:methods; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D007313:Insecta; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009928:Organ Specificity; D011505:Protein-Tyrosine Kinases / Q000235:genetics*; D016392:Proto-Oncogene Proteins pp60(c-src) / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D012689:Sequence Homology, Nucleic Acid; D014162:Transfection; D019061:src-Family Kinases
D012333:RNA, Messenger; D004247:DNA; D011505:Protein-Tyrosine Kinases; D000081247:CSK Tyrosine-Protein Kinase; D016392:Proto-Oncogene Proteins pp60(c-src); D019061:src-Family Kinases