Partial release of aminopeptidase N from larval midgut cell membranes of the silkworm, Bombyx mori, by phosphatidylinositol-specific phospholipase C.
1. The membrane anchor of aminopeptidase N associated with larval midgut cell membranes of the silkworm, Bombyx mori, was investigated by using phosphatidylinositol-specific phospholipase C (PIPLC) and proteases. 2. Aminopeptidase N, which was virtually all localized in the brush border membrane, was solubilized by PIPLC but not by papain or trypsin. 3. Detergent-solubilized amphiphilic aminopeptidase N was converted into a hydrophilic form by PIPLC but not by papain. 4. Either of these effects of PIPLC on aminopeptidase N was maximally 40%. 5. These results suggest that in larval midgut cells of the silkworm, B. mori, at least 40% aminopeptidase N is anchored in the brush border membrane via glycosyl-phosphatidylinositol.
College of Medical Technology, Nagoya University, Japan.
S
S
Takesue
K
K
Yokota
S
S
Miyajima
R
R
Taguchi
H
H
Ikezawa
Y
Y
Takesue
D000626:Aminopeptidases / Q000378:metabolism*; D000818:Animals; D012831:Bombyx / Q000201:enzymology* / Q000378:metabolism; D018826:CD13 Antigens; D002462:Cell Membrane / Q000378:metabolism; D004064:Digestive System / Q000378:metabolism; D006017:Glycolipids / Q000378:metabolism; D017261:Glycosylphosphatidylinositols; D007814:Larva / Q000378:metabolism; D008871:Microvilli / Q000378:metabolism; D043265:Phosphatidylinositol Diacylglycerol-Lyase; D010716:Phosphatidylinositols / Q000378:metabolism; D054801:Phosphoinositide Phospholipase C; D010727:Phosphoric Diester Hydrolases / Q000494:pharmacology
D006017:Glycolipids; D017261:Glycosylphosphatidylinositols; D010716:Phosphatidylinositols; D010727:Phosphoric Diester Hydrolases; D054801:Phosphoinositide Phospholipase C; D000626:Aminopeptidases; D018826:CD13 Antigens; D043265:Phosphatidylinositol Diacylglycerol-Lyase