Failure of tetrodotoxin to inhibit the prostaglandin-induced secretory response of rat small intestine in-vitro.
Tetrodotoxin (TTX, 10 microM) did not inhibit prostaglandin E2 (PGE2)-stimulated increases in electrical activity in intact or stripped sheets of rat small intestine, although it reduced basal electrical activity in the intact preparation. Basal and PGE2-stimulated cAMP production by enterocytes isolated from the small intestine was unaffected by TTX. Thus its reported ability to inhibit prostaglandin-induced fluid secretion in-vivo does not appear to represent a direct interaction of the neurotoxin with the mechanism of prostaglandin action at the enterocyte.
Hardcastle
J
J
Department of Biomedical Science, University of Sheffield, UK.
Hardcastle
P T
PT
D000818:Animals; D000242:Cyclic AMP / Q000032:analysis; D015232:Dinoprostone / Q000494:pharmacology*; D066298:In Vitro Techniques; D007421:Intestine, Small / Q000378:metabolism*; D008297:Male; D051381:Rats; D013779:Tetrodotoxin / Q000494:pharmacology*
D013779:Tetrodotoxin; D000242:Cyclic AMP; D015232:Dinoprostone