Transgenic production of a variant of human tissue-type plasminogen activator in goat milk: generation of transgenic goats and analysis of expression.
We report the first successful production of transgenic goats that express a heterologous protein in their milk. The production of a glycosylation variant of human tPA (LAtPA--longer acting tissue plasminogen activator) from an expression vector containing the murine whey acid promoter (WAP) operatively linked to the cDNA of a modified version of human tPA was examined in transgenic dairy goats. Two transgenic goats were identified from 29 animals born. The first animal, a female, was mated and allowed to carry the pregnancy to term. Milk was obtained upon parturition and was shown to contain enzymatically active LAtPA at a concentration of 3 micrograms/ml.
Ebert
K M
KM
Tufts University School of Veterinary Medicine, North Grafton, MA 01536-1895.
Selgrath
J P
JP
DiTullio
P
P
Denman
J
J
Smith
T E
TE
Memon
M A
MA
Schindler
J E
JE
Monastersky
G M
GM
Vitale
J A
JA
Gordon
K
K
D000818:Animals; D030801:Animals, Genetically Modified; D015139:Blotting, Southern; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D004624:Embryo Transfer; D005260:Female; D015870:Gene Expression; D014644:Genetic Variation; D006041:Goats / Q000235:genetics*; D006801:Humans; D008892:Milk / Q000201:enzymology*; D011247:Pregnancy; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000302:isolation & purification; D015183:Restriction Mapping; D010959:Tissue Plasminogen Activator / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification
D011994:Recombinant Proteins; D004247:DNA; D010959:Tissue Plasminogen Activator