Identification of a novel vertebrate homeobox gene expressed in haematopoietic cells.
This paper describes the characterisation of a novel chicken homeobox gene, Prh, whose encoded homeodomain sequence differs significantly from those of other factors which have been described. As expected, a portion of the encoded protein, containing the homeodomain, is capable of sequence-specific DNA-binding. Outside the homeodomain, Prh, possesses an N-terminal region extremely rich in proline residues and a C-terminal acidic portion, either of which may function as transcription regulatory domains. Since, among the chicken tissues tested, its transcription is restricted to haematopoietic cells, lung and liver, it may function in tissue-specific patterns of gene regulation. Human and murine Prh homologues have also been identified; so it is likely that such genes are a general feature of vertebrate genomes.
Chester Beatty Laboratories, Institute of Cancer Research, London, UK.
M R
MR
Crompton
T J
TJ
Bartlett
A D
AD
MacGregor
G
G
Manfioletti
E
E
Buratti
V
V
Giancotti
G H
GH
Goodwin
D000595:Amino Acid Sequence; D000818:Animals; D009189:Avian Myeloblastosis Virus / Q000502:physiology; D001483:Base Sequence; D015152:Blotting, Northern; D002460:Cell Line; D002472:Cell Transformation, Viral; D002642:Chick Embryo; D004247:DNA; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism*; D003851:Deoxyribonucleases / Q000378:metabolism; D005801:Genes, Homeobox; D006412:Hematopoietic Stem Cells / Q000378:metabolism*; D018398:Homeodomain Proteins; D006801:Humans; D008099:Liver / Q000378:metabolism*; D008168:Lung / Q000378:metabolism*; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D014157:Transcription Factors; D014714:Vertebrates / Q000235:genetics
D004268:DNA-Binding Proteins; C078492:HHEX protein, human; D018398:Homeodomain Proteins; D014157:Transcription Factors; D004247:DNA; D003851:Deoxyribonucleases