Effect of ethanol on ascorbate release in the nucleus accumbens and striatum of freely moving rats.
An in vivo voltammetry technique was used to monitor the extracellular ascorbate (AA) concentration in the nucleus accumbens and striatum of unanesthetized, freely moving rats. A single injection of ethanol, 1.0 g/kg intraperitoneally (IP), induced a significant increase in extracellular AA concentration in both the nucleus accumbens and striatum. This effect was dose dependent within a dose range from 0.5-2.0 g/kg. 4-Methylpyrazole (50 mg/kg, IP), which inhibits alcoholdehydrogenase, could not prevent the increase in AA concentration, evoked by ethanol. Furthermore, systemic administration of acetaldehyde (20 mg/kg, IP), the main metabolite of ethanol, did not have any effect on the level of AA in the nucleus accumbens or striatum. These results show that ethanol can alter the brain extracellular AA levels and that this effect seems to be attributed to ethanol itself and not to acetaldehyde. Consequently, these results indicate that a role for AA in the action of ethanol in the brain should be considered.
Department of Pharmacology, University of Göteborg, Sweden.
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L
Svensson
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C
Wu
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Johannessen
J A
JA
Engel
D000818:Animals; D001205:Ascorbic Acid / Q000378:metabolism*; D003342:Corpus Striatum / Q000187:drug effects* / Q000378:metabolism; D000431:Ethanol / Q000633:toxicity*; D000077604:Fomepizole; D005971:Glutamates / Q000378:metabolism; D018698:Glutamic Acid; D008297:Male; D009714:Nucleus Accumbens / Q000187:drug effects* / Q000378:metabolism; D011720:Pyrazoles / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley
D005971:Glutamates; D011720:Pyrazoles; D000431:Ethanol; D018698:Glutamic Acid; D000077604:Fomepizole; D001205:Ascorbic Acid