Antibody production in baculovirus-infected insect cells.
We have employed the baculovirus expression system for the production of a mouse monoclonal IgG antibody directed against lipoprotein I of Pseudomonas aeruginosa. Both light and heavy chain cDNAs were introduced into the baculovirus genome in a single step of homologous recombination. Insect cells that were infected with the recombinant virus stably secreted antigen-binding and glycosylated antibody molecules capable of binding the complement component C1q.
zu Putlitz
J
J
Laboratorium für molekulare Biologie-Genzentrum-der Ludwig-Maximilians-Universität München, Martinsried, FRG.
Kubasek
W L
WL
Duchêne
M
M
Marget
M
M
von Specht
B U
BU
Domdey
H
H
D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology / Q000378:metabolism; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000378:metabolism*; D016367:Baculoviridae / Q000235:genetics*; D003001:Cloning, Molecular / Q000379:methods*; D003172:Complement C1 / Q000276:immunology; D005822:Genetic Vectors; D006031:Glycosylation; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000276:immunology / Q000378:metabolism; D007143:Immunoglobulin Heavy Chains / Q000096:biosynthesis / Q000378:metabolism; D007147:Immunoglobulin Light Chains / Q000096:biosynthesis / Q000378:metabolism; D007313:Insecta / Q000166:cytology*; D008074:Lipoproteins / Q000276:immunology; D051379:Mice; D011550:Pseudomonas aeruginosa / Q000276:immunology*; D011994:Recombinant Proteins / Q000276:immunology / Q000378:metabolism
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D003172:Complement C1; D007074:Immunoglobulin G; D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains; D008074:Lipoproteins; D011994:Recombinant Proteins