Isolation of a new human pseudogene for proliferating cell nuclear antigen.
A new gene, which cross-hybridized with a rat PCNA cDNA probe, has been isolated from a human genomic cosmid library. A comparison of the gene with the human PCNA cDNA revealed 71% homology for the nucleotide sequences. This gene completely lacks introns and has traces of a polyA tail which the messenger RNA of the active gene retains. These facts indicate that this gene was generated by the reverse-transcription of a processed RNA for PCNA and exists as a PCNA pseudogene in the human genome.
Division of Molecular Life Science (Cell Biology), Tokai University School of Medicine, Kanagawa, Japan.
Y
Y
Taniguchi
Y
Y
Katsumata
S
S
Koido
S
S
Yoshimura
H
H
Suemizu
T
T
Moriuchi
D000818:Animals; D003001:Cloning, Molecular; D004247:DNA; D006801:Humans; D009687:Nuclear Proteins / Q000235:genetics*; D018809:Proliferating Cell Nuclear Antigen; D011544:Pseudogenes; D051381:Rats; D015183:Restriction Mapping
D009687:Nuclear Proteins; D018809:Proliferating Cell Nuclear Antigen; D004247:DNA