Relationship between cell-cycle state of erythroid progenitors and fetal hemoglobin synthesis.
DNA-synthesis state of circulating burst forming unit-erythroid (BFU-E) was evaluated in patients with sickle cell anemia and correlated with percent of fetal hemoglobin synthesized in the BFU-E-derived cells. Percentage of S-phase BFU-E inversely correlated with percent fetal hemoglobin synthesized in the BFU-E-derived cells (simple linear correlation coefficient, r = -0.8, P = 0.0302; polynomial regression, R = -0.99, P = 0.0002). This observation is of relevance to our understanding of the relationship between the developmental stage of the erythroid progenitors and expression of globin genes.
University of South Alabama Comprehensive Sickle Cell Center, Mobile.
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D002453:Cell Cycle / Q000502:physiology; D002478:Cells, Cultured; D004247:DNA / Q000235:genetics / Q000378:metabolism; D015672:Erythroid Precursor Cells / Q000166:cytology* / Q000378:metabolism; D005319:Fetal Hemoglobin / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D013936:Thymidine / Q000378:metabolism; D014316:Tritium
D014316:Tritium; D004247:DNA; D005319:Fetal Hemoglobin; D013936:Thymidine