Endonuclease-free, protoplast-forming enzyme preparation and its application in fungal transformation.
An endonuclease-free, protoplast-forming enzyme complex was prepared from the "snail enzyme." The purified preparation has high protoplast-forming activity comparable to the crude enzyme complex without destroying circular plasmid DNA. Furthermore, a higher transformation rate was achieved by the application of the endonuclease-free enzyme complex in both yeast and filamentous fungal vector-host systems.
Mink
M
M
Department of Microbiology, Attila József University, Szeged, Hungary.
Höltke
H J
HJ
Kessler
C
C
Ferenczy
L
L
D000818:Animals; D001230:Aspergillus / Q000235:genetics / Q000648:ultrastructure*; D003851:Deoxyribonucleases / Q000032:analysis; D004720:Endonucleases / Q000302:isolation & purification*; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D009097:Multienzyme Complexes / Q000302:isolation & purification* / Q000378:metabolism; D010957:Plasmids; D011523:Protoplasts / Q000648:ultrastructure*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000648:ultrastructure*; D012908:Snails / Q000201:enzymology; D014170:Transformation, Genetic
D009097:Multienzyme Complexes; D003851:Deoxyribonucleases; D004720:Endonucleases