The effect of Pluronic F-68 on hybridoma cells in continuous culture.
The comparative effects of Pluronic on cell growth of hybridomas were studied. The addition of 0.05% Pluronic decreased the steady-state cell number in continuous culture by about 12% compared to a non-supplemented culture. In short-term experiments, results demonstrated a gradual decrease in cell number with increasing concentration of Pluronic. Such growth inhibition was found to be a result of lowering the rate of DNA synthesis.
Centre for Biochemical Engineering, School of Chemical Engineering, University of Birmingham, UK.
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al-Rubeai
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Emery
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Chalder
D000818:Animals; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D006825:Hybridomas / Q000187:drug effects*; D051379:Mice; D011060:Poloxalene / Q000494:pharmacology*
D011060:Poloxalene