Comparison of Clostridium sordellii toxins HT and LT with toxins A and B of C. difficile.
Clostridium sordellii produces two toxins, designated HT (haemorrhagic toxin) and LT (lethal toxin), that are similar to toxins A and B of C. difficile. The physicochemical properties of toxins HT and A were remarkably similar. The specific biological activities of toxin HT were almost the same as those of toxin A, and their NH2-terminal sequences shared close homology. The properties of toxins LT and B were similar, as were their NH2-terminal sequences, but toxin B was much more cytotoxic than toxin LT. Immunodiffusion analysis with specific antibodies showed that although toxins B and LT shared major antigenic determinants, each had unique epitopes. The results suggest that toxins B and LT have diverged more than toxins A and HT. Immunoblotting with antibodies to the toxins of C. difficile showed that toxins HT and LT had common antigenic determinants.
Department of Anaerobic Microbiology, Virginia Polytechnic Institute and State University, Blacksburg 24061.
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Martinez
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Wilkins
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D001426:Bacterial Proteins; D001427:Bacterial Toxins / Q000737:chemistry* / Q000276:immunology / Q000302:isolation & purification; D016360:Clostridioides difficile / Q000737:chemistry*; D003013:Clostridium / Q000737:chemistry*; D004768:Enterotoxins / Q000737:chemistry* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes; D006386:Hemagglutination Tests; D007122:Immunoelectrophoresis; D008969:Molecular Sequence Data; D010449:Peptide Mapping
D000596:Amino Acids; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004768:Enterotoxins; D000939:Epitopes; C072293:hemorrhagic toxin HT, Clostridium sordellii; C072292:lethal toxin LT, Clostridium sordellii; C041348:tcdA protein, Clostridium difficile; C031815:toxB protein, Clostridium difficile