Antibody production in silkworm cells and silkworm larvae infected with a dual recombinant Bombyx mori nuclear polyhedrosis virus.
We have examined the efficiency of coexpression of two heterologous genes from a recombinant Bombyx mori nuclear polyhedrosis virus for the production of antibodies in silkworm larvae. The cDNAs encoding the light and the heavy chains of a murine immunoglobulin, directed against lipoprotein I of Pseudomonas aeruginosa, were brought under the control of two separate copies of the viral polyhedrin promotor. Infection of silkworm cells with the recombinant baculovirus yielded a maximum of 6.4 micrograms/ml IgG2A in the culture supernatant 72 hours post infection, while 800 micrograms/ml IgG2A was found in the hemolymph of infected fifth instar silkworm larvae seven days after infection with the same construct. The recombinant antibody exhibited a similar antigen specificity and avidity to that of the monoclonal antibody derived from ascites fluid.
Dept. of Genetics, GBF, Gesellschaft für Biotechnologische Forschung mbH, Braunschweig, Germany.
U
U
Reis
B
B
Blum
B U
BU
von Specht
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H
Domdey
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J
Collins
D000818:Animals; D000906:Antibodies / Q000235:genetics*; D000915:Antibody Affinity; D000918:Antibody Specificity; D000942:Antigens, Bacterial / Q000276:immunology; D001426:Bacterial Proteins / Q000276:immunology; D016367:Baculoviridae / Q000235:genetics*; D012831:Bombyx / Q000276:immunology* / Q000382:microbiology; D004247:DNA / Q000235:genetics; D015870:Gene Expression; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000235:genetics; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D007147:Immunoglobulin Light Chains / Q000235:genetics; D007814:Larva / Q000276:immunology / Q000382:microbiology; D008074:Lipoproteins / Q000276:immunology; D011401:Promoter Regions, Genetic; D011550:Pseudomonas aeruginosa / Q000737:chemistry / Q000276:immunology; D011994:Recombinant Proteins / Q000276:immunology
D000906:Antibodies; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D007074:Immunoglobulin G; D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains; D008074:Lipoproteins; D011994:Recombinant Proteins; C060507:outer membrane lipoprotein I, Bacteria; D004247:DNA