Immunoregulation of lymphoproliferation in vitro by monocytes and their subpopulations. II. Phenotypic changes of T cells in cultures activated with antigen and mitogen.
The aim of this study was to analyze phenotypes of T cells activated by mitogen (PWM) and antigen (PPD) in the presence of FcR+ or FcR- monocytes. It was found that CD4+ and CD8+ lymphocytes are preferentially activated in the presence of different monocyte subpopulations. Expression of HLA-DR and CD25 on CD4+ lymphocytes was greater in cultures activated in the presence of FcR-. CD8+ lymphocytes were more efficiently activated (expression of HLA-DR) when FcR+ monocytes were added to culture. In the presence of FcR+ monocytes an increased expression of CD45RA antigen on CD4+ cells was also observed. These data support our previous functional studies which showed that "suppressor" T cells of CD8+ phenotype are activated in the presence of FcR+ monocytes.
Pryjma
J
J
Department of Clinical Immunology and Microbiology, Copernicus Medical School, Cracow, Poland.
Kowalczyk
D
D
Ruggiero
I
I
Zembala
M
M
D015704:CD4 Antigens / Q000032:analysis; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D016827:CD8 Antigens / Q000032:analysis; D002478:Cells, Cultured; D006684:HLA-DR Antigens / Q000032:analysis; D006801:Humans; D016130:Immunophenotyping; D017493:Leukocyte Common Antigens / Q000032:analysis; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000276:immunology*; D008213:Lymphocyte Activation / Q000187:drug effects; D011043:Pokeweed Mitogens / Q000494:pharmacology; D015375:Receptors, Interleukin-2 / Q000032:analysis; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology; D014373:Tuberculin / Q000494:pharmacology
D015704:CD4 Antigens; D016827:CD8 Antigens; D006684:HLA-DR Antigens; D011043:Pokeweed Mitogens; D015375:Receptors, Interleukin-2; D014373:Tuberculin; D017493:Leukocyte Common Antigens