Differential susceptibility of phosphatidylcholine small unilamellar vesicles to phospholipases A2, C and D in the presence of membrane active peptides.
Activities of phospholipases C and D along with A2 were followed on egg phosphatidylcholine small unilamellar vesicles in the presence of membrane active peptides melittin, gramicidin S and alamethicin. Decrease in the activity of phospholipase C and D and enhancement of phospholipases A2 activity suggest that these enzymes are sensitive to alterations in the lipid packing in the membranes in the presence of these peptides. Phospholipase C and D, which have not been used to study peptide--membrane interactions, have potential use in studying membrane perturbations, since their activities are very sensitive to lipid packing.
Centre for Cellular and Molecular Biology, Hyderabad, India.
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Rao
D000408:Alamethicin / Q000494:pharmacology*; D000818:Animals; D001409:Bacillus cereus / Q000201:enzymology; D001937:Brassica / Q000201:enzymology; D006096:Gramicidin / Q000494:pharmacology*; D007700:Kinetics; D008081:Liposomes; D008555:Melitten / Q000494:pharmacology*; D010179:Pancreas / Q000201:enzymology; D010713:Phosphatidylcholines / Q000378:metabolism*; D010739:Phospholipase D / Q000378:metabolism*; D010741:Phospholipases A / Q000378:metabolism*; D013552:Swine; D010738:Type C Phospholipases / Q000378:metabolism*
D008081:Liposomes; D010713:Phosphatidylcholines; D006096:Gramicidin; D008555:Melitten; D000408:Alamethicin; D010741:Phospholipases A; D010738:Type C Phospholipases; D010739:Phospholipase D