Microscopic visualization of insect cell-bubble interactions. I: Rising bubbles, air-medium interface, and the foam layer.
Through the use of microscopic, high-speed video technology, the interactions of two suspended insect cell lines, Trichoplusia ni (TN-368) and Spodoptera frugiperda (SF-9), with air and oxygen bubbles were studied. Events such as cell-bubble attachment, cell-bubble collision, cell transport into the foam layer, and trapping of cells in the foam layer are presented and discussed. Based on these observations and those in a companion paper (Chalmers, J. J.; Bavarian, F. Biotechnol. Prog. 1991, following paper in this issue) and the experimental and theoretical work of other researchers, several mechanisms of cell damage as a result of sparging are presented.
Department of Chemical Engineering, Ohio State University, Columbus 43210.
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Bavarian
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Fan
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Chalmers
D000388:Air; D000818:Animals; D002448:Cell Adhesion; D002460:Cell Line; D002465:Cell Movement; D003470:Culture Media; D007313:Insecta / Q000166:cytology*; D008853:Microscopy / Q000379:methods*; D010100:Oxygen; D014741:Video Recording
D003470:Culture Media; D010100:Oxygen