Structure and chromosomal localization of the human 2',3'-cyclic nucleotide 3'-phosphodiesterase gene.
Human brain cDNA clones for the myelin associated enzyme 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNPase) have been isolated and sequenced. The only 5' untranslated region (UTR) sequence found was that of a human CNPII mRNA, with no direct evidence for a CNPI mRNA. Human CNPase cDNAs were used to isolate genomic clones containing the human CNPase gene which is 9 kb long. Four exons were identified, separated by three introns, and the sequence of each exon and intron/exon boundary has been established. The polymerase chain reaction (PCR) was used to detect the presence of the human CNPase gene in DNA from a panel of rodent/human somatic cell hybrids. By this means the human CNPase gene was mapped to chromosome 17. In situ hybridization of a human CNPase genomic clone to metaphase chromosomes further localized this gene to chromosomal band 17q21.
University Clinical Biochemistry, Southampton General Hospital, U.K.
A J
AJ
Douglas
M F
MF
Fox
C M
CM
Abbott
L J
LJ
Hinks
G
G
Sharpe
S
S
Povey
R J
RJ
Thompson
D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000201:enzymology; D002871:Chromosome Banding; D002886:Chromosomes, Human, Pair 17; D004247:DNA; D006801:Humans; D006822:Hybrid Cells; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012333:RNA, Messenger / Q000235:genetics; D015183:Restriction Mapping; D012377:Rodentia
D012333:RNA, Messenger; D004247:DNA; D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases