A reliable method for the use of oligonucleotides as probes in blot-hybridization experiments.
We have developed a ligation and specific-primer radiolabeling method that allows the use of oligonucleotides as probes in blot-hybridization experiments. The major advantage of the protocol is that standard hybridization and washing conditions may be used and yield high signals and low background. The observed increase in the stability and intensity of the hybridization signals appears to result from both increased length and specific radioactivity of the hybridization probe.
Paquette
J
J
Ludwig Institute for Cancer Research, Montreal, Quebec, Canada.
Sapienza
C
C
D001483:Base Sequence; D006358:Hot Temperature; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes / Q000138:chemical synthesis*; D012150:Polymorphism, Restriction Fragment Length
D015345:Oligonucleotide Probes