Induced RAW 264.7 macrophages express soluble and particulate nitric oxide synthase: inhibition by transforming growth factor-beta.
RAW 264.7 macrophages induced with lipopolysaccharide and interferon-gamma expressed nitric oxide (NO) synthase. Approximately two-thirds of the total induced NO synthase activity was found in the cytosolic fraction, whereas one-third was associated with the particulate fraction. Both enzymes formed L-citrulline in addition to NO-like material. NO and L-citrulline formation by both enzymes were calcium-independent and inhibited by NG-nitro-L-arginine and NG-methyl-L-arginine. Transforming growth factor-beta 1 prevented the induction of both enzymes.
Förstermann
U
U
Abbott Laboratories, Abbott Park, IL 60064.
Schmidt
H H
HH
Kohlhaas
K L
KL
Murad
F
F
D000594:Amino Acid Oxidoreductases / Q000378:metabolism*; D002478:Cells, Cultured; D002956:Citrulline / Q000096:biosynthesis; D007371:Interferon-gamma / Q000494:pharmacology; D008070:Lipopolysaccharides; D008264:Macrophages / Q000187:drug effects / Q000201:enzymology*; D019001:Nitric Oxide Synthase; D016212:Transforming Growth Factor beta / Q000494:pharmacology*
D008070:Lipopolysaccharides; D016212:Transforming Growth Factor beta; D002956:Citrulline; D007371:Interferon-gamma; D019001:Nitric Oxide Synthase; D000594:Amino Acid Oxidoreductases