Cloning of three endoglucanase genes from Thermomonospora curvata into Escherichia coli.
A BamHI genomic library from Thermomonospora curvata was constructed in E. coli using cosmid vector pHC79. Four clones able to hydrolyze CMC were isolated. Restriction digests and Southern gel analysis revealed the presence of three different endoglucanase genes. DNA fragments contained in all of the endoglucanase cosmids hybridized to T. curvata chromosomal DNA. The cellulase genes were expressed in E. coli, but at rather low levels.
Presutti
D G
DG
Department of Microbiology, Clemson University, South Carolina 29634-1909.
Hughes
T A
TA
Stutzenberger
F J
FJ
D001419:Bacteria / Q000235:genetics*; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics*; D002480:Cellulase / Q000096:biosynthesis / Q000235:genetics*; D003001:Cloning, Molecular; D003360:Cosmids; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial; D011994:Recombinant Proteins / Q000096:biosynthesis
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D011994:Recombinant Proteins; D002480:Cellulase