The murine Hox-2.4 promoter contains a functional octamer motif.
Like other HOX genes the murine Hox-2.4 gene is thought to be involved in regional specification along the antero-posterior axis. In addition it has been reported to have oncogenic potential. We studied expression Hox-2.4 in murine EC cells and determined the transcription start site. Studies of DNA-protein interactions in the promoter region showed that the Hox-2.4 promoter contains a CCAAT box and a perfect octamer motif, which is capable of binding Oct-factors. Cotransfection of Oct expression vectors influences the transcriptional activity of the promoter, suggesting that the Oct-gene family may be involved in regulating HOX genes.
Hubrecht Laboratory, Netherlands Institute for Developmental Biology, Utrecht.
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Zwartkruis
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Hoeijmakers
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Deschamps
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Meijlink
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D015152:Blotting, Northern; D054278:Embryonal Carcinoma Stem Cells; D005786:Gene Expression Regulation / Q000235:genetics*; D005801:Genes, Homeobox / Q000235:genetics*; D005822:Genetic Vectors / Q000235:genetics; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D014411:Neoplastic Stem Cells; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic / Q000235:genetics*
D011993:Recombinant Fusion Proteins; D014157:Transcription Factors; D008156:Luciferases