Direct nucleotide sequencing using total cellular RNA from dengue-virus-infected mosquito cells.
In recent years, a large amount of nucleotide sequence data for dengue viruses has been published. Most of it was derived by sequencing cDNA synthesized from highly purified genomic viral RNA. This paper presents a simple and rapid method for the isolation of total RNA from mosquito cells infected with dengue viruses. This RNA can be used for direct nucleotide sequencing with specific primers without the need for further purification.
Kautner
I M
IM
Dept. of Medical Microbiology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
Lam
S K
SK
D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D009033:Culicidae / Q000166:cytology / Q000235:genetics*; D003716:Dengue Virus / Q000235:genetics*; D008969:Molecular Sequence Data; D012367:RNA, Viral / Q000235:genetics* / Q000302:isolation & purification
D012367:RNA, Viral