Coiled tube membrane bioreactor for cultivation of hybridoma cells producing monoclonal antibodies.
A coiled tube membrane reactor was developed for the cultivation of mouse-mouse hybridoma cells producing monoclonal antibodies. The cell and antibody concentrations obtained in the membrane reactor were higher than that obtained in a batch spinner flask without a membrane. A mathematical model has been developed to describe the membrane transport coupled growth and product formation, and the physical and kinetic constants of the system were determined.
Hagedorn
J
J
Department of Chemical Engineering, Washington University, St. Louis, Missouri 63130.
Kargi
F
F
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D002455:Cell Division; D002460:Cell Line; D002470:Cell Survival; D046508:Culture Techniques / Q000295:instrumentation* / Q000379:methods; D006825:Hybridomas / Q000166:cytology* / Q000276:immunology; D007700:Kinetics; D051379:Mice; D008954:Models, Biological
D000911:Antibodies, Monoclonal