Mapping genetic determinants for human immunodeficiency virus type 1 resistance to soluble CD4.
Neutralization of human immunodeficiency virus type 1 (HIV-1) infection with soluble CD4 (sCD4) can be achieved over a broad range of concentrations for different virus strains. Laboratory virus strains passaged in transformed T-cell lines are typically sensitive to sCD4 neutralization, whereas primary virus isolates require over 100-fold-higher sCD4 concentrations. Using recombinant viruses generated from a laboratory strain, HIV-1NL4-3, and a primary macrophagetropic strain, HIV-1JR-FL, we mapped a region of gp120 important for determining sensitivity to sCD4 neutralization. This same region has previously been defined as important for macrophage and transformed T-cell line tropism and includes the V3 neutralization domain but does not include regions of gp120 that have been shown to be most important for CD4 binding.
Department of Medicine, West Los Angeles Veteran's Administration Medical Center, California.
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O'Brien
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Chen
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Ho
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Daar
D000163:Acquired Immunodeficiency Syndrome / Q000235:genetics* / Q000517:prevention & control; D000937:Antigen-Antibody Reactions; D001665:Binding Sites; D015704:CD4 Antigens / Q000378:metabolism*; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D000939:Epitopes / Q000235:genetics* / Q000378:metabolism; D015699:HIV Envelope Protein gp120 / Q000235:genetics* / Q000378:metabolism; D015497:HIV-1 / Q000235:genetics* / Q000472:pathogenicity; D006801:Humans; D009928:Organ Specificity; D016133:Polymerase Chain Reaction; D011995:Recombination, Genetic
D015704:CD4 Antigens; D000939:Epitopes; D015699:HIV Envelope Protein gp120