Depletion of T-lymphocyte subsets in murine herpes-simplex-virus retinitis.
Uniocular injection of herpes-simplex virus type 1 into the anterior chamber of BALB/c mice induced contralateral retinitis with relative preservation of the ipsilateral retina. Overall 95% of T-cell deficient nude mice developed ipsi- and contralateral retinitis, suggesting the importance of T-cells in this model. We then depleted lymphocyte subsets in susceptible BALB/c and resistant CB-17 and C57BL/6J mice using anti-CD4 (helper/inducer cells) or anti-CD8 (suppressor/cytotoxic cells) monoclonal antibody. 85% of CD8-depleted, 58% of CD4-depleted and 50% of untreated BALB/c mice developed contralateral retinitis. All CD4- and CD8-depleted animals developed severe ipsilateral retinitis. These results suggest that CD8 cells (but not CD4 cells) are protective for the contralateral retina in BALB/c mice and that both subsets are important for the ipsilateral protection. In CB-17 and C57BL/6J mice, depletion produced no change in the contralateral retina but resulted in ipsilateral retinitis, suggesting different mechanisms for ipsi- and contralateral protection. The possible role of the anterior-chamber-associated immune deviation is discussed.
Massachusetts Eye and Ear Infirmary, Hilles Immunology Laboratory, Harvard Medical School, Boston.
M
M
Zierhut
S
S
Soukasian
T Z
TZ
Zhao
R R
RR
Tamesis
C S
CS
Foster
D000818:Animals; D000867:Anterior Chamber / Q000382:microbiology; D000911:Antibodies, Monoclonal; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D004198:Disease Susceptibility; D015828:Eye Infections, Viral / Q000276:immunology* / Q000473:pathology; D005260:Female; D005434:Flow Cytometry; D006561:Herpes Simplex / Q000276:immunology* / Q000473:pathology; D007113:Immunity, Innate; D015994:Incidence; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008819:Mice, Nude; D012173:Retinitis / Q000276:immunology* / Q000382:microbiology / Q000473:pathology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*
D000911:Antibodies, Monoclonal