D-ribulokinase from Klebsiella pneumoniae for continuous production of D-(-)-ribulose-5-phosphate.
The production of D-ribulose-5-phosphate in an enzyme membrane reactor was examined. Phosphoryl transfer from ATP to D-ribulose was catalysed by D-ribulokinase isolated from Klebsiella pneumoniae. For production of D-ribulose-5-phosphate the phosphoryl donor ATP was used either in stoichiometric or in catalytic amounts. Using catalytic amounts of ATP requires a second enzyme, e.g. pyruvate kinase, to regenerate ATP. The kinetic parameters for D-ribulokinase and pyruvate kinase were determined to calculate the performance of an enzyme membrane reactor for continuous production of D-ribulose-5-phosphate. Both processes operated for more than 200 h. Regardless of whether ATP was used in catalytic or stoichiometric amounts, about the same production parameters were determined. In continuous production space/time yields of 117 g (with ATP regeneration) and 103 g (without ATP regeneration) of D-ribulose-5-phosphate l -1 per day were reached.
Central Research Laboratories of Ciba-Geigy Ltd., Basel, Switzerland.
D
D
Gygax
H
H
Nachtegaal
O
O
Ghisalba
R
R
Lattmann
H P
HP
Schär
C
C
Wandrey
M B
MB
Streiff
D000255:Adenosine Triphosphate / Q000378:metabolism; D001426:Bacterial Proteins / Q000302:isolation & purification* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D007711:Klebsiella pneumoniae / Q000201:enzymology*; D010428:Pentosephosphates / Q000138:chemical synthesis*; D010770:Phosphotransferases / Q000302:isolation & purification* / Q000378:metabolism; D017853:Phosphotransferases (Alcohol Group Acceptor); D011770:Pyruvate Kinase / Q000378:metabolism; D012274:Ribulosephosphates / Q000138:chemical synthesis*
D001426:Bacterial Proteins; D010428:Pentosephosphates; D012274:Ribulosephosphates; C031524:ribulose 5-phosphate; D000255:Adenosine Triphosphate; D010770:Phosphotransferases; D017853:Phosphotransferases (Alcohol Group Acceptor); C022550:ribulokinase; D011770:Pyruvate Kinase