[Activity of Ca(+2)Mg(+2) --ATPase in erythrocyte membranes of women with diabetes mellitus type I].
The activity of Ca(++)-Mg++ ATP-ase present in erythrocyte membranes was determined in basal conditions and following stimulation with calmodulin in 8 women with insulin-dependent diabetes and in 9 healthy women. The isolation of erythrocyte membranes and the determination of activity of Ca(++)-Mg(++)-ATP-ase were carried out according to the method of Gietzen et al. A decrease in the activity of Ca(++)-Mg(++)-ATP-ase in basal conditions was found in fractions with the highest erythrocyte content obtained from diabetic patients. After stimulation with calmodulin the activity of Ca(++)-Mg(++)-ATP-ase in all the fractions was lower in diabetic patients than in the controls. Low activities of the enzyme were accompanied by high values of HbA1c. The results suggest that glycosylation of the ATP-ase or/and calmodulin may be the main cause of the observed fall in the enzyme activity in diabetes. Also the disturbances concerning the cumulation of intracellular calcium may be related to the changes caused by glycosylation of Ca(++)-Mg(++)-ATP-ase or/and calmodulin.
Klinika Endokrynologii AM, Białymstoku.
M
M
Szelachowska
W
W
Schaefer
A F
AF
Gries
I
I
Kinalska
D000328:Adult; D017301:Ca(2+) Mg(2+)-ATPase / Q000378:metabolism*; D002147:Calmodulin / Q000494:pharmacology; D003922:Diabetes Mellitus, Type 1 / Q000201:enzymology*; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000378:metabolism*; D005260:Female; D006442:Glycated Hemoglobin / Q000032:analysis; D006801:Humans
D017301:Ca(2+) Mg(2+)-ATPase; D002147:Calmodulin; D006442:Glycated Hemoglobin
Aktywność Ca+2Mg(+2)--ATP-azy w błonach erytrocytarnych u kobiet chorych na cukrzyce typu I.