The use of tissue culture for the detection of mycotoxins.
After incubation with 20 different mycotoxin standards and extracts from fungi and feed stuffs, fluorescent flow cytometry was used to measure viability of NS-1 cells and compared to microscopic assessment of cytotoxicity on stained HEp-11 monolayers. Both methods gave essentially the same results but the cytometric analysis offered a more quantitative approach and was particularly appropriate in the screening of extracts containing fats. The potential uses of a cytotoxic test in the analysis of feed stuffs and fungal extracts for the presence of mycotoxins are discussed.
Department of Microbiology, East of Scotland College of Agriculture, Edinburgh, UK.
J
J
Robb
M
M
Norval
W A
WA
Neill
D000818:Animals; D001681:Biological Assay / Q000379:methods*; D002470:Cell Survival; D005434:Flow Cytometry; D006801:Humans; D009183:Mycotoxins / Q000032:analysis* / Q000633:toxicity; D014407:Tumor Cells, Cultured
D009183:Mycotoxins