Regeneration of the eighth cranial nerve in the bullfrog, Rana catesbeiana.
The present study was done in order to document the ability of the eighth cranial nerve of the bullfrog (Rana catesbeiana) to regenerate, the anatomic characteristics of the regenerated fibers, and the specificity of projections from individual endorgan branches of the nerve. The eighth cranial nerve was sharply transected between the ganglion cells and the brain stem in 40 healthy bullfrogs and allowed to regenerate. Anatomic studies were performed in these animals a minimum of 3 months postoperatively. Horseradish peroxidase was used to label the whole vestibular nerve or its individual endorgan branches. Labeled regenerated fibers could be identified crossing the site of the nerve section and projecting centrally to the vestibular nuclei in a pattern similar to that of normal frogs. Labeling of individual branches showed that regenerated fibers innervated the same specific areas found in normal frogs. Unlike normal animals, both thick and thin fibers projected to the medial nucleus.
Division of Head and Neck Surgery, UCLA School of Medicine 90024.
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Newman
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Honrubia
D000818:Animals; D001370:Axonal Transport; D006735:Horseradish Peroxidase; D009412:Nerve Fibers / Q000502:physiology / Q000648:ultrastructure; D009416:Nerve Regeneration; D009474:Neurons / Q000166:cytology / Q000502:physiology; D011892:Rana catesbeiana; D014722:Vestibule, Labyrinth / Q000502:physiology; D000159:Vestibulocochlear Nerve / Q000166:cytology / Q000502:physiology*
D006735:Horseradish Peroxidase