Development of a piezoelectric immunosensor for the detection of Salmonella typhimurium.
A piezoelectric biosensor has been developed for the detection of Salmonella typhimurium. The antibody to Salmonella was immobilized on the crystal by various immobilization procedures. The best result was obtained when antibody was immobilized on the crystal precoated with a thin layer of polyethyleneimine. The response of the coated crystal for S. typhimurium in a microbial suspension was in the range of 10(5) to 10(9) cells ml-1. The time required for a complete interaction between the crystal and the cells appeared to depend upon the cell concentration of the analyzed sample. The antibody-bound crystal lost no activity over 4 days at 4 degrees C and it could be reused for 6-8 consecutive assays.
National Research Council of Canada, Biotechnology Research Institute, Montreal, Quebec.
E
E
Prusak-Sochaczewski
J H
JH
Luong
G G
GG
Guilbault
D000907:Antibodies, Bacterial; D015374:Biosensing Techniques; D004566:Electrodes; D005516:Food Microbiology; D007202:Indicators and Reagents; D008722:Methods; D012486:Salmonella typhimurium / Q000302:isolation & purification*
D000907:Antibodies, Bacterial; D007202:Indicators and Reagents