Genetic engineering of Trichoderma to produce strains with novel cellulase profiles.
Genetic engineering has been used to modify the proportion of different cellulases produced by a hypercellulolytic Trichoderma reesei mutant strain. A general expression vector, pAMH110, containing the promoter and terminator sequences of the strongly expressed main cellobiohydrolase 1 (cbh1) gene was used to overexpress a cDNA coding for EGI, the major endoglucanase (1,4,beta-D-glucan glucanohydrolase, EC 3.2.1.4). An in vitro modified cbh1 cDNA, incapable of coding for active enzyme, was used to inactivate the major cellobiohydrolase (1,4-beta-D-glucan cellobiohydrolase, EC 3.2.1.91) gene. In this way, new strains producing elevated amounts of the specific endoglucanase 1 (EGI) and/or lacking the major cellobiohydrolase (CBHI) were produced, and these have been further characterized.
Harkki
A
A
VTT Biotechnical Laboratory, Espoo, Finland.
Mäntylä
A
A
Penttilä
M
M
Muttilainen
S
S
Bühler
R
R
Suominen
P
P
Knowles
J
J
Nevalainen
H
H
D001483:Base Sequence; D002482:Cellulose / Q000096:biosynthesis / Q000235:genetics*; D003001:Cloning, Molecular / Q000379:methods; D005818:Genetic Engineering / Q000379:methods; D008969:Molecular Sequence Data; D010957:Plasmids; D015183:Restriction Mapping; D014242:Trichoderma / Q000201:enzymology / Q000235:genetics*
D002482:Cellulose