Determination of diphenylmethane antihistaminic drugs and their analogues in body fluids by gas chromatography with surface ionization detection.
Eleven diphenylmethane antihistaminic drugs and their analogues were tested for their detection by capillary gas chromatography (GC) with surface ionization detection (SID). The GC-SID response was highest for doxylamine, diphenhydramine and orphenadrine and lowest for terodiline, clemastine and pipethanate. The detection limits for drugs with the highest response were 2-5 pg (ca. 6-20 fmol) on-column (100-250 pg/ml of body fluid). The detection limits with GC-SID were 10-100 times higher than those with GC with nitrogen-phosphorus detection. A detailed procedure for the isolation of the antihistaminics from human whole blood and urine by the use of Sep-Pak C18 cartridges, prior to GC-SID, is also presented. The recoveries of the drugs (50 or 500 pmol), which had been added to 1 ml of body fluids, were > 60%. The baselines remained steady as the column temperature was increased and the background was clean, especially for whole blood extracts.
Department of Legal Medicine, Aichi Medical University, Japan.
H
H
Hattori
S
S
Yamamoto
M
M
Iwata
E
E
Takashima
T
T
Yamada
O
O
Suzuki
D001559:Benzhydryl Compounds / Q000032:analysis* / Q000097:blood / Q000652:urine; D002849:Chromatography, Gas / Q000379:methods*; D006634:Histamine H1 Antagonists / Q000032:analysis* / Q000097:blood / Q000652:urine; D006801:Humans; D007477:Ions
D001559:Benzhydryl Compounds; D006634:Histamine H1 Antagonists; D007477:Ions; C010129:diphenylmethane