Galanin reduces PDBu-induced protein phosphorylation in rat ventral hippocampus.
The effect of galanin (GAL) on basal and phorbol-12,13-dibutyrate (PDBu) induced protein phosphorylation in rat ventral hippocampal miniprisms was investigated. GAL (0.5, 1 and 2 microM) inhibited PDBu stimulation in a concentration-dependent manner without altering basal protein phosphorylation. This inhibitory effect was prevented by the GAL antagonist galantide. GAL did not affect either the activity of protein kinase C (PKC) from rat brain or basal phosphorylation in ventral hippocampal hippogenates, suggesting that it did not directly modulate PKC activity. Depolarization of miniprisms from ventral hippocampi by 18 mM K+ prevented the effect of GAL on PDBu-induced phosphorylation. The results indicate that GAL indirectly regulates neuronal protein phosphorylation by a GAL receptor-mediated action.
Istituto di Ricerche Farmacologiche Mario Negri, Milano, Italy.
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La Porta
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Bianchi
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Sozzani
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Bartfai
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Consolo
D000818:Animals; D019004:Galanin / Q000031:analogs & derivatives; D006624:Hippocampus / Q000378:metabolism*; D008297:Male; D009479:Neuropeptides / Q000502:physiology*; D010455:Peptides / Q000502:physiology*; D015240:Phorbol 12,13-Dibutyrate / Q000494:pharmacology*; D010766:Phosphorylation; D011506:Proteins / Q000378:metabolism*; D051381:Rats; D013373:Substance P / Q000031:analogs & derivatives*
D009479:Neuropeptides; D010455:Peptides; D011506:Proteins; C074975:galantide; D013373:Substance P; D015240:Phorbol 12,13-Dibutyrate; D019004:Galanin