Microbial modification of sugars as building blocks for chemicals.
Investigations on the microbial modification of sucrose to the corresponding 3-keto-derivative were carried out with resting cells of Agrobacterium tumefaciens NCPPB 396. This highly specific oxidation to yield the 3-keto-derivative has been analysed kinetically with varying substrate and cell mass concentrations. The formation of the corresponding 3-keto-derivative depended strongly on the reaction time and the aeration rate and was maximal at aeration rates up to 11.5 volume air/cultivation volume per minute with resting cells. The product formation increased with increasing substrate concentrations. However, the product yield was maximal at substrate concentrations below 20 g/l. Data pertaining to the production of active cell mass as well as for maximal 3-keto-derivative formation are presented in this paper. Also included are some applications for these derivatives.
Stoppok
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E
Institut fur landwirtschaftliche Technologie und Zuckerindustrie an der TU Braunschweig, Langer Kamp 5, Germany.
Matalla
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K
Buchholz
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K
D000332:Aerobiosis; D016960:Agrobacterium tumefaciens / Q000187:drug effects / Q000378:metabolism*; D003470:Culture Media; D005296:Ferrous Compounds / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008345:Manganese / Q000494:pharmacology; D013395:Sucrose / Q000031:analogs & derivatives* / Q000378:metabolism*; D013997:Time Factors
D003470:Culture Media; D005296:Ferrous Compounds; C059956:3-ketosucrose; D008345:Manganese; D013395:Sucrose