Improvement in the antibody productivity of human-human hybridomas by transfection with Tac gene.
The presence of a highly purified recombinant interleukin-2 (rIL-2) increased the production of immunoglobulin (IgM or IgG) by human-human hybridomas to 1.5-2.0 times the production by untreated cells. However, these cells did not react with anti-Tac (IL-2 receptor alpha) antibody. To enhance the response of the hybridoma cells to rIL-2, Tac gene was introduced by co-transfection with Tac gene expression plasmid pTB459 and G418 resistant gene expression plasmid pRSVneo. Tac cDNA transfected hybridoma (HBW-4.16.459-6-126) was induced to produce 6 times as much IgG by rIL-2 as was the control. This antibody production promoting phenomenon mediated by rIL-2 was depressed by anti-Tac antibody.
Microbiology Research Laboratories, Takeda Chemical Industries, Ltd., Osaka, Japan.
K
K
Iwamoto
Y
Y
Shintani
R
R
Sasada
T
T
Honjo
K
K
Kitano
D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D005818:Genetic Engineering; D006801:Humans; D006825:Hybridomas / Q000378:metabolism*; D007074:Immunoglobulin G / Q000096:biosynthesis; D007376:Interleukin-2 / Q000502:physiology; D015375:Receptors, Interleukin-2 / Q000235:genetics*; D011994:Recombinant Proteins; D014162:Transfection
D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D007376:Interleukin-2; D015375:Receptors, Interleukin-2; D011994:Recombinant Proteins