CD3.TCR1, a human CD3 epitope expressed on viable gamma/delta lymphocytes exclusively.
T lymphocytes express either the alpha/beta or the gamma/delta receptor (TCR) in a mutually exclusive fashion. Both structures are associated on the cell membrane with the CD3 proteins which are thought to transduce signals resulting from antigen recognition. The CD3 complex is present in both alpha/beta and gamma/delta cells and includes at least five proteins (designated gamma, delta, epsilon, zeta and eta). We have developed here a novel mAb, anti-CD3.TCR1, which immunoprecipitates the CD3 molecules from both alpha/beta and gamma/delta cells lysates following solubilization with Triton X-100. While the SDS-PAGE migration profile of the material recognized by either anti-CD3.TCR1 or anti-OKT3 are superimposable in both cell types, this mAb recognizes viable untreated gamma/delta T lymphocytes exclusively. These findings further support the view that molecular interactions within the TCR/CD3 protein complex are distinct in the two T lymphocyte populations.
Laboratoire d'Hémato-immunologie, INSERM U333, Institut Gustave-Roussy, Villejuif, France.
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Miossec
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Genevee
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Hercend
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Jitsukawa
D000818:Animals; D000906:Antibodies; D000945:Antigens, Differentiation, T-Lymphocyte / Q000008:administration & dosage / Q000737:chemistry / Q000276:immunology*; D017252:CD3 Complex; D002460:Cell Line; D002999:Clone Cells; D000939:Epitopes / Q000276:immunology; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D006801:Humans; D016131:Lymphocyte Subsets / Q000276:immunology; D008562:Membrane Glycoproteins / Q000276:immunology*; D016853:Muromonab-CD3 / Q000096:biosynthesis / Q000276:immunology; D011233:Precipitin Tests; D011948:Receptors, Antigen, T-Cell / Q000737:chemistry / Q000276:immunology*; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*
D000906:Antibodies; D000945:Antigens, Differentiation, T-Lymphocyte; D017252:CD3 Complex; D000939:Epitopes; D008562:Membrane Glycoproteins; D016853:Muromonab-CD3; D011948:Receptors, Antigen, T-Cell; D016692:Receptors, Antigen, T-Cell, gamma-delta