Micronucleus tests on N-ethyl-N-nitrosourea with mouse peripheral blood reticulocytes using acridine orange-coated slides.
The micronucleus test with peripheral blood using acridine orange-coated slides was validated in male CD-1 mice treated once with N-ethyl-N-nitrosourea (ENU) at doses of 6.25, 12.5, 25.0, and 50.0 mg/kg body weight. Peripheral blood preparations were made 0, 24, 48, and 72 h after treatment. The frequencies of micronucleated peripheral reticulocytes in the ENU-treated groups increased dose-dependently, peaking at 48 h after treatment. The results indicate that the method used in the present study can be an alternative to the method using bone marrow polychromatic erythrocytes.
Hitotsumachi
S
S
Drug Safety Research Laboratories, Takeda Chemical Industries, Ltd., Osaka, Japan.
Kimura
Y
Y
Katoh
M
M
Ishihara
N
N
Hara
T
T
Shibuya
T
T
D000165:Acridine Orange; D000818:Animals; D004305:Dose-Response Relationship, Drug; D005038:Ethylnitrosourea / Q000633:toxicity*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D015162:Micronucleus Tests / Q000379:methods; D009153:Mutagens / Q000633:toxicity*; D012156:Reticulocytes / Q000187:drug effects*
D009153:Mutagens; D000165:Acridine Orange; D005038:Ethylnitrosourea