Recombinant E-selectin-protein mediates tumor cell adhesion via sialyl-Le(a) and sialyl-Le(x).
E-selectin (previously known as ELAM-1) is one of the adhesion molecules expressed on activated endothelium. Here we show that HL-60 cells express sialyl-Le(x), but not Sialyl-Le(a) on their surface, a colon carcinoma cell line COLO 205 express both these epitopes and another colon carcinoma COLO 320 does not express either one of them. HL-60 and COLO 205 cell adhere strongly to E-selectin coated microwells, whereas COLO 320 does not adhere at all to E-selectin. Finally we provide evidence that monoclonal anti-sialyl-Le(x) can abolish part of the adherence of HL-60 cells to recombinant E-selectin. The adherence of COLO 205 cells can be decreased by either monoclonal anti-sialyl-Le(a) or anti-sialyl-Le(x) antibodies. These results indicate that cell-associated sialylated carbohydrate moieties can act as ligands for recombinant E-selectin.
Department of Bacteriology and Immunology, University of Helsinki, Finland.
M L
ML
Majuri
P
P
Mattila
R
R
Renkonen
D000911:Antibodies, Monoclonal; D002448:Cell Adhesion / Q000502:physiology*; D015815:Cell Adhesion Molecules / Q000276:immunology*; D002460:Cell Line; D003110:Colonic Neoplasms; D019040:E-Selectin; D005434:Flow Cytometry; D006801:Humans; D015473:Leukemia, Promyelocytic, Acute; D007983:Lewis Blood Group Antigens / Q000502:physiology*; D011971:Receptors, Immunologic / Q000502:physiology; D011994:Recombinant Proteins / Q000494:pharmacology
D000911:Antibodies, Monoclonal; D015815:Cell Adhesion Molecules; D019040:E-Selectin; D007983:Lewis Blood Group Antigens; D011971:Receptors, Immunologic; D011994:Recombinant Proteins