Mutagenicity of the reaction products of dibenzo-p-dioxin with nitrogen oxides.
Dibenzo-p-dioxin (DD) was made to react with various concentrations of nitrogen oxides in the dark. The mutagenicities of the reaction products were tested using Salmonella typhimurium strains TA98, TA100, TA98NR and TA98/1,8-DNP6 in the presence or absence of a mammalian metabolic activation system (S9 mix). DD-NOx (molar ratios 1:3, 1:6 and 1:18) reaction products exhibited mutagenic potency in strains TA98 and TA98/1,8-DNP6 without S9 mix. In a gas chromatography/mass spectrometry study, 2-nitrodibenzo-p-dioxin (NDD) was identified with authentic sample in the mutagenic reaction products. DD-NOx (1:18) reaction products were reduced by sodium hydrogen sulfide and the reduction mixture was analyzed by HPLC. 2,7-Dinitrodibenzo-p-dioxin (DNDD) and 2,8-DNDD were identified as corresponding diamino-DDs in the reduction mixture. 2-NDD, 2,7-DNDD and 2,8-DNDD were also mutagenic in strains TA98 and TA98/1,8-DNP6 without S9 mix and the mutagenicity of DD-NOx reaction products was largely accounted for by the nitro-DDs.
Kyoto Pharmaceutical University, Japan.
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T
Watanabe
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Kusumoto
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M
Ikeda
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T
Hirayama
D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D004147:Dioxins / Q000737:chemistry / Q000633:toxicity*; D008401:Gas Chromatography-Mass Spectrometry; D009682:Magnetic Resonance Spectroscopy; D009153:Mutagens / Q000633:toxicity*; D009589:Nitrogen Oxides / Q000737:chemistry*; D012486:Salmonella typhimurium / Q000187:drug effects
D004147:Dioxins; D009153:Mutagens; D009589:Nitrogen Oxides; C020887:dibenzo(1,4)dioxin