Studies on carbohydrates extracted from native and chemically treated Biomphalaria alexandrina snails.
1. Carbohydrates were extracted from total tissue extracts of Biomphalaria alexandrina snails and were analyzed to their monosaccharides using GLC. 2. The snails were chemically treated with thioxanthone derivatives (compounds I, II, III) and the change in the monosaccharide constituents of their carbohydrates was investigated. 3. The isolated monosaccharides from native and chemically pretreated snails were injected into mice and their protective effects were examined after infection of mice with cercariae of Schistosoma mansoni. 4. The results showed that the main monosaccharides in carbohydrates of snails were galactose, glucose, fucose and mannose and that chemical treatment caused a drop in the galactose content. 5. Moreover, monosaccharide fractions from snails treated with compound III were the most effective in inducing protection against Schistosoma infection in mice.
Nabih
I
I
Medicinal Chemistry Department, National Research Centre, Cairo, Egypt.
Rizk
M
M
Soliman
A M
AM
D000818:Animals; D001700:Biomphalaria / Q000737:chemistry* / Q000187:drug effects* / Q000276:immunology; D002241:Carbohydrates / Q000737:chemistry / Q000302:isolation & purification; D006826:Hycanthone / Q000494:pharmacology*; D007422:Intestines / Q000469:parasitology; D007928:Lethal Dose 50; D008099:Liver / Q000469:parasitology; D008154:Lucanthone / Q000494:pharmacology*; D051379:Mice; D009005:Monosaccharides / Q000276:immunology* / Q000302:isolation & purification; D010270:Parasite Egg Count; D012555:Schistosomiasis mansoni / Q000276:immunology / Q000517:prevention & control*; D013892:Thioxanthenes / Q000494:pharmacology*; D014612:Vaccines / Q000276:immunology; D044004:Xanthones
D002241:Carbohydrates; D009005:Monosaccharides; D013892:Thioxanthenes; D014612:Vaccines; D044004:Xanthones; D006826:Hycanthone; C027484:4-(beta-diethylaminoethylamino)-1,2-cyclohexenothiaxanthone; D008154:Lucanthone