Selective activation of murine V beta 8.2 bearing T cells by Pseudomonas exotoxin A.
We have determined that Pseudomonas aeruginosa exotoxin A (PE) can selectively stimulate the proliferation of V beta bearing T lymphocytes. Murine thymocytes were fractionated by selective agglutination with peanut agglutinin (PNA) and the PNA- thymocytes, which represent mature thymocytes, were shown to be responsive to PE stimulation. In addition, mature peripheral T lymphocytes (nylon wool nonadherent splenocytes) were also observed to respond to PE stimulation. Both CD4+ and CD8+ splenic T lymphocyte populations proliferated in response to PE. Flow microfluorimetry analysis of PNA- thymocytes stimulated with PE indicated that V beta 8.2 bearing T cells were preferentially expanded. Thus, our data indicate that PE represents a microbial super antigen which stimulates murine thymocytes which bear the V beta 8.2 element of the T cell receptor.
Department of Molecular Microbiology and Immunology, University of Missouri-Columbia, School of Medicine 65212.
D M
DM
Dixon
R D
RD
LeGrand
M L
ML
Misfeldt
D036002:ADP Ribose Transferases; D000818:Animals; D001427:Bacterial Toxins; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D016827:CD8 Antigens / Q000032:analysis; D002478:Cells, Cultured; D005098:Exotoxins / Q000276:immunology* / Q000502:physiology; D008213:Lymphocyte Activation; D051379:Mice; D011550:Pseudomonas aeruginosa / Q000378:metabolism*; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000032:analysis*; D013601:T-Lymphocytes / Q000276:immunology*; D037521:Virulence Factors; D000097668:Pseudomonas aeruginosa Exotoxin A
D036002:ADP Ribose Transferases; D001427:Bacterial Toxins; D016827:CD8 Antigens; D005098:Exotoxins; D016693:Receptors, Antigen, T-Cell, alpha-beta; D037521:Virulence Factors; D000097668:Pseudomonas aeruginosa Exotoxin A