Characterization of peptidyl-nucleoside antifungal antibiotics from fermentation broth.
Characterization of sinefungin related antifungal antibiotics from fermentation broth was accomplished by coupling photodiode array (PDA) detection to high performance liquid chromatography (HPLC). From the combined HPLC-PDA evaluation of broth filtrate, we detected five sinefungin related components. Fast atom bombardment (FAB) mass spectroscopic evaluations, mass-analysed ion kinetic energy spectra (MIKES) and collision activated (CA) MIKES of these components confirmed their respective identities. Our findings from the combination of HPLC photodiode array acquisition and FAB-mass spectrometry suggest we have detected the presence of a previously unreported sinefungin analogue.
Cooper
R
R
Pharmaceutical Research Division, Schering Plough Corporation, Bloomfield, NJ.
Das
P
P
Federbush
C
C
Mierzwa
R
R
Patel
M
M
Pramanik
B
B
Truumees
I
I
D000241:Adenosine / Q000031:analogs & derivatives* / Q000032:analysis / Q000302:isolation & purification; D000935:Antifungal Agents / Q000032:analysis* / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D002855:Chromatography, Thin Layer; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D013058:Mass Spectrometry; D015394:Molecular Structure
D000935:Antifungal Agents; D000241:Adenosine; C006235:sinefungin