The hemolytic activity of deoxynivalenol and T-2 toxin.
The hemolytic effects of deoxynivalenol (DON) and T-2 toxin (T-2) individually on rat erythrocytes were studied at different concentrations. Sodium azide was used as an enzyme inhibitor to prevent T-2 toxin metabolism. The concentration of T-2 was controlled by GC-MS and no decrease of the toxin was found during the time of the experiment. In spite of the much higher toxicity of T-2 toxin to eucaryotic cells, DON and T-2 showed similar lytic activity toward erythrocytes at high and low concentrations. Neither of these toxins at a concentration of 130 micrograms/ml, produced significant hemolysis even after 11 hr incubation. This finding suggests that there is a threshold level for both T-2 and DON, below which the lytic reaction does not occur. An additional hemolysis test was conducted in the presence of mannitol, glutathione, ascorbic acid, alfa-tocopherol, and histidine. The assay demonstrated that all the compounds inhibited to some extent the hemolytic reaction of the toxins. It is suggested that DON and T-2 exert their toxicity on procaryotic cells in three different ways: by penetrating the phospholipid bilayer and acting at the subcellular level, by interacting with the cellular membranes, and by free radical mediated phospholipid peroxidation. Most probably, more than one mechanism operates at the same time.
Department of Chemistry, National Veterinary Institute, Helsinki, Finland.
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Rizzo
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Atroshi
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Hirvi
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Saloniemi
D000818:Animals; D005609:Free Radicals; D006461:Hemolysis / Q000187:drug effects*; D008297:Male; D010743:Phospholipids / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013605:T-2 Toxin / Q000633:toxicity*; D014255:Trichothecenes / Q000633:toxicity*
D005609:Free Radicals; D010743:Phospholipids; D014255:Trichothecenes; D013605:T-2 Toxin; C007262:deoxynivalenol