Flow cytometric analysis of the expression of murine B and T surface markers from birth to adulthood.
The proportion of nucleated splenocytes bearing B-lymphocyte markers B220, surface IgM (sIgM) and sIgD, as well as the T-lymphocyte markers Thy 1.2, CD5, CD8a and CD4 were quantitated by flow cytometric analysis (FACS) throughout postpartum development in the A/J mouse. Full expression of B lymphocyte markers was achieved much sooner than expression of T lymphocyte markers. This was especially true for B220, which was found on 8% of all splenocytes at day 5 and reached adult levels (47-50%) by weaning at day 22. Expression of sIgM and sIgD were 13% and 9%, respectively, of all splenocytes at day 5 with mature levels not expressed until day 35 postpartum (approximately 36% of cells were positive for these markers). T lymphocyte markers, on the other hand, did not reach full expression until sexual maturity. For example, Thy 1.2 expression was 8% on day 5 and did not reach mature levels (28-30%) until day 56. CD5 closely paralleled Thy 1.2 expression rising from only 2% on day 5 to 27% by day 56. Likewise, CD8a and CD4 marker development paralleled one another with CD8a rising from 1% on day 5 to 10% by day 56 and CD4 rising from 5% on day 5 to 19% by day 56. These data demonstrate the variability in the time of appearance and rate of maturation of the various lymphocyte cell surface markers during postpartum development. They also serve as a reference to identify alterations in lymphocyte development created by immunodeficiency diseases.
Department of Biochemistry, Michigan State University, East Lansing 48824.
L E
LE
King
L A
LA
Morford
J P
JP
Gibbons
P J
PJ
Fraker
D000375:Aging / Q000276:immunology*; D000818:Animals; D015703:Antigens, CD / Q000276:immunology; D000954:Antigens, Surface / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology; D005434:Flow Cytometry; D008214:Lymphocytes / Q000276:immunology*; D008562:Membrane Glycoproteins / Q000276:immunology; D008565:Membrane Proteins / Q000276:immunology*; D051379:Mice; D013601:T-Lymphocytes / Q000276:immunology; D018800:Thy-1 Antigens
D015703:Antigens, CD; D000954:Antigens, Surface; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D018800:Thy-1 Antigens