Alleles of the rat T-cell receptor beta chain gene complex.
Inbred rat strains provide a rich source of genetic diversity in immunologically relevant genes. We have characterized the alleles of one of these genes, encoding the rat T-cell receptor C beta 1 chain, by Southern blots and nucleic acid sequencing. The Cb1 gene segments from DA and LEW rats display complex allotypic variation: both coding and noncoding regions contain multiple nucleotide substitutions. In addition, there is a polymorphic insertion of a rat repetitive LINE element 3' to the coding region. The Cb1 alleles are one part of larger Tcrb haplotypes, containing V beta, D beta, and J beta elements; complete Cb1 genomic nucleotide sequences, and a partial list of the strain distribution of the two alleles, are described in this report.
Department of Microbiology and Immunology, Hahnemann University, Philadelphia, PA 19102-1192.
E P
EP
Blankenhorn
P D
PD
Smith
C B
CB
Williams
G A
GA
Gutman
D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002874:Chromosome Mapping; D015342:DNA Probes; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D051381:Rats; D011919:Rats, Inbred Strains / Q000235:genetics*; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
D015342:DNA Probes; D016693:Receptors, Antigen, T-Cell, alpha-beta