Nucleotide sequence and PCR-amplification of a polymorphic MboI site in human DNA marker D4S95 linked to the Huntington disease locus.
The nucleotide sequence of the probe BS674E-D defining the genetic locus D4S95 was assessed, and used for designing PCR-primers amplifying a 175 bp fragment containing a polymorphic MboI site. With the described conditions it is now possible to use PCR followed by digestion for rapid determination of genotype in this marker system.
Institute of Medical Genetics, University of Copenhagen, Denmark.
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D001483:Base Sequence; D015342:DNA Probes; D015252:Deoxyribonucleases, Type II Site-Specific; D005838:Genotype; D006801:Humans; D006816:Huntington Disease / Q000235:genetics*; D008969:Molecular Sequence Data; D010375:Pedigree; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D017422:Sequence Analysis, DNA / Q000379:methods*
D015342:DNA Probes; D015252:Deoxyribonucleases, Type II Site-Specific; C024405:GATC-specific type II deoxyribonucleases