Microbial transformation of immunosuppressive compounds. I. Desmethylation of FK506 and immunomycin (FR 900520) by Actinoplanes sp. ATCC 53771.
The immunosuppressants FK506 and FR 900520 were desmethylated by Actinoplanes sp. ATCC 53771 to yield various O-desmethylated products. The products were isolated and purified by solvent extraction and HPLC chromatography, and identified by NMR and MS spectroscopy.
Merck Sharp & Dohme Research Laboratory, Rahway, New Jersey 07065.
T S
TS
Chen
B H
BH
Arison
L S
LS
Wicker
E S
ES
Inamine
R L
RL
Monaghan
D000192:Actinomycetales / Q000378:metabolism*; D000818:Animals; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D005285:Fermentation; D007166:Immunosuppressive Agents / Q000737:chemistry / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008752:Methylene Chloride; D051379:Mice; D008968:Molecular Conformation; D015394:Molecular Structure; D010880:Piperidines / Q000737:chemistry / Q000378:metabolism*; D016339:Spectrometry, Mass, Fast Atom Bombardment; D013601:T-Lymphocytes / Q000187:drug effects; D016559:Tacrolimus / Q000737:chemistry / Q000378:metabolism* / Q000031:analogs & derivatives
D007166:Immunosuppressive Agents; D010880:Piperidines; D008752:Methylene Chloride; C058028:immunomycin; D016559:Tacrolimus