IL-10 is produced by subsets of human CD4+ T cell clones and peripheral blood T cells.
Murine IL-10 has been reported originally to be produced by the Th2 subset of CD4+ T cell clones. In this study, we demonstrate that human IL-10 is produced by Th0, Th1-, and Th2-like CD4+ T cell clones after both Ag-specific and polyclonal activation. In purified peripheral blood T cells, low, but significant, levels of IL-10 were found to be produced by the CD4+CD45RA+ population, whereas CD4+CD45RA- "memory" cells secreted 5- to 20-fold higher levels of IL-10. In addition, IL-10 was produced by activated CD8+ peripheral blood T cells. Optimal induction of IL-10 was observed after activation by specific Ag and by the combination of anti-CD3 mAb and the phorbol ester tetradecanoyl phorbol acetate, whereas the combination of calcium ionophore A23187 and 12-O-tetradecanoylphorbol-13-acetate acetate was a poor inducer of IL-10 production. Kinetic studies indicated that IL-10 was produced relatively late as compared with other cytokines. Maximal IL-10 mRNA expression in CD4+ T cell clones and purified peripheral blood T cells was obtained after 24 h, whereas maximal IL-10 protein synthesis occurred between 24 h and 48 h after activation. No differences were observed in the kinetics of IL-10 production among Th0, Th1-, and Th2-like subsets of CD4+ T cell clones. The results indicate a regulatory role for IL-10 in later phases of the immune response.
Human Immunology Department, DNAX Research Institute, Palo Alto, CA 94304-1104.
H
H
Yssel
R
R
De Waal Malefyt
M G
MG
Roncarolo
J S
JS
Abrams
R
R
Lahesmaa
H
H
Spits
J E
JE
de Vries
D015703:Antigens, CD / Q000032:analysis; D015496:CD4-Positive T-Lymphocytes / Q000378:metabolism*; D016827:CD8 Antigens / Q000032:analysis; D002999:Clone Cells; D006649:Histocompatibility Antigens / Q000032:analysis; D006801:Humans; D016753:Interleukin-10 / Q000096:biosynthesis*; D017493:Leukocyte Common Antigens; D013601:T-Lymphocytes / Q000378:metabolism*; D006377:T-Lymphocytes, Helper-Inducer / Q000378:metabolism
D015703:Antigens, CD; D016827:CD8 Antigens; D006649:Histocompatibility Antigens; D016753:Interleukin-10; D017493:Leukocyte Common Antigens