Production of hepatitis B surface antigen (preS2 + S) by high-cell density cultivations of a recombinant yeast.
A high-cell density bioprocess has been developed for the production of hepatitis B surface protein (preS2 + S) by recombinant yeast. This fed-batch process utilizes a growth medium containing yeast extract, soy peptone and glucose which was fed at a constant rate to maintain cells in a respiratory state. Cell densities of up to 60 g l-1 dry weight were achieved, which represented a 6-fold increase over those from batch bioprocesses. This increase in cell mass was attained without compromising specific activity; therefore, volumetric productivities of six times those of batch bioprocesses were achieved.
Department of Cellular and Molecular Biology, Merck Sharp and Dohme Research Laboratories, West Point, Pennsylvania 19486.
C A
CA
Schulman
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RW
Ellis
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RZ
Maigetter
D006514:Hepatitis B Surface Antigens / Q000096:biosynthesis*; D011498:Protein Precursors / Q000096:biosynthesis*; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D014759:Viral Envelope Proteins / Q000096:biosynthesis*
D006514:Hepatitis B Surface Antigens; D011498:Protein Precursors; D014759:Viral Envelope Proteins; C050807:presurface protein 2, hepatitis B surface antigen