Bacterial degradation of 3-chloroacrylic acid and the characterization of cis- and trans-specific dehalogenases.
A coryneform bacterium that is able to utilize cis- and trans-3-chloroacrylic acid as sole carbon source for growth was isolated from freshwater sediment. The organism was found to produce two inducible dehalogenases, one specific for the cis- and the other for trans-3-chloroacrylic acid. Both dehalogenases were purified to homogeneity from cells induced for dehalogenase synthesis with 3-chlorocrotonic acid. The enzymes produced muconic acid semialdehyde (3-oxopropionic acid) from their respective 3-chloroacrylic acid substrate. No other substrates were found. The cis-3-chloroacrylic acid dehalogenase consisted of two polypeptide chains of a molecular weight 16.2 kDa. Trans-3-chloroacrylic acid dehalogenase was a protein with subunits of 7.4 and 8.7 kDa. The subunit and amino acid compositions and the N-terminal amino acid sequences of the enzymes indicate that they are not closely related.
Department of Biochemistry, Groningen Biotechnology Center, University of Groningen, The Netherlands.
J E
JE
van Hylckama Vlieg
D B
DB
Janssen
D000179:Acrylates / Q000737:chemistry / Q000378:metabolism*; D000192:Actinomycetales / Q000302:isolation & purification / Q000378:metabolism*; D000595:Amino Acid Sequence; D001673:Biodegradation, Environmental; D001709:Biotechnology; D006867:Hydrolases / Q000737:chemistry / Q000378:metabolism*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011487:Protein Conformation; D013237:Stereoisomerism; D013379:Substrate Specificity; D014871:Water Microbiology
D000179:Acrylates; C071773:3-chloroacrylic acid; D006867:Hydrolases; C077719:cis-3-chloroacrylic acid dehalogenase; C077721:trans-3-chloroacrylic acid dehalogenase