The influence of ras oncogene expression on radiation response in the Rat-1 cell.
We evaluated the effect of H-ras oncogene expression on resistance to ionizing radiation in cultured rat fibroblasts. The Rat-1 cell line, and two Rat-1 derivatives, MR4 and MR7, carrying a ZN-regulatable metallothionein-rasT24 fusion gene were used to study the effects of the ras oncogene on radiation sensitivity. Cells were irradiated with a 137Cs source (450 cGY/min) in the presence or absence of ZnSO4. Multiple cell survival studies did not show an appreciable difference in sensitivity to radiation among the lines in the presence or absence of ras oncogene expression.
Department of Experimental Radiotherapy, University of Texas M.D. Anderson Cancer Center, Houston 77030.
A S
AS
Garden
R E
RE
Meyn
M M
MM
Weil
R M
RM
Lebovitz
M W
MW
Lieberman
D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D005347:Fibroblasts / Q000528:radiation effects; D015870:Gene Expression; D011905:Genes, ras; D011836:Radiation Tolerance / Q000235:genetics*; D051381:Rats