Receptor autoradiography with 11C and [3H]-labelled ligands visualized by imaging plates.
The distribution of histamine H1, H3, dopamine D1 and D2 receptors in the brain was studied by receptor autoradiography using a high-sensitivity and high-resolution imaging plate system. [3H]Pyrilamine, [3H](R)alpha-methyl-histamine, [11C]SCH23390, and [11C]N-methylspiperone (or [11C]YM-09151-2) were used as ligands to identify H1, H3, D1 and D2 receptors, respectively. Two different receptors (dopamine D2 and histamine H3) could be also labelled simultaneously in a single cryostat-sliced section using [11C]N-methylspiperone and [3H](R)alpha-methylhistamine, respectively. The imaging plate system is useful for receptor autoradiography of positron emitter-labelled and tritium-labelled receptor-ligands because of its high sensitivity.
Department of Pharmacology I, Tohoku University School of Medicine, Sendai, Japan.
K
K
Yanai
J H
JH
Ryu
T
T
Watanabe
R
R
Iwata
T
T
Ido
D000818:Animals; D001345:Autoradiography; D001921:Brain / Q000000981:diagnostic imaging; D015259:Dopamine Agents; D065127:Dopamine D2 Receptor Antagonists; D006168:Guinea Pigs; D017442:Histamine Agonists; D006633:Histamine Antagonists; D008024:Ligands; D008297:Male; D008761:Methylhistamines; D051381:Rats; D017208:Rats, Wistar; D011956:Receptors, Cell Surface / Q000037:antagonists & inhibitors / Q000378:metabolism*; D017447:Receptors, Dopamine D1 / Q000037:antagonists & inhibitors / Q000378:metabolism; D017448:Receptors, Dopamine D2 / Q000378:metabolism; D011968:Receptors, Histamine / Q000378:metabolism; D018100:Receptors, Histamine H3; D013134:Spiperone / Q000031:analogs & derivatives; D014055:Tomography, Emission-Computed
D015259:Dopamine Agents; D065127:Dopamine D2 Receptor Antagonists; D017442:Histamine Agonists; D006633:Histamine Antagonists; D008024:Ligands; D008761:Methylhistamines; D011956:Receptors, Cell Surface; D017447:Receptors, Dopamine D1; D017448:Receptors, Dopamine D2; D011968:Receptors, Histamine; D018100:Receptors, Histamine H3; D013134:Spiperone; C069357:alpha-methylhistamine; C039167:3-N-methylspiperone