Protein A insensitive ELISA detection of staphylococcal enterotoxin B.
A sandwich enzyme-linked immunosorbent assay to detect staphylococcal enterotoxin B was developed using rat monoclonal antibodies as capture antibodies and as a biotinylated conjugate. This test was sensitive, less than 1 ng/ml of enterotoxin B was detected and interference by protein A was prevented by the use of rat monoclonal antibodies of the IgG2a isotype which were insensitive to protein A even at concentrations greater than 1000 ng/ml.
Centre d'Immunochimie Microbienne, Institut Pasteur de Lyon, L'Arbresle, France.
V
V
Rogemond
J M
JM
Da Costa Castro
T
T
Delaunay
C
C
Cazaux
R M
RM
Guinet
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D001710:Biotin; D003429:Cross Reactions; D004768:Enterotoxins / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007074:Immunoglobulin G / Q000276:immunology; D011237:Predictive Value of Tests; D051381:Rats; D013205:Staphylococcal Protein A; D013211:Staphylococcus aureus
D000911:Antibodies, Monoclonal; D004768:Enterotoxins; D007074:Immunoglobulin G; D013205:Staphylococcal Protein A; C031818:enterotoxin B, staphylococcal; D001710:Biotin