Use of fluorescence anisotropy determinations for indicating the physiological status of hybridoma cell cultures.
The evolution of lipid compartment fluidity during culture of hybridoma cells was studied by fluorescence polarization measurements. The probe partition between the plasma membrane and intracytoplasmic compartments was determined by a quenching fluorescence method. A progressive decrease of the plasma membrane fluidity was observed during the growth phase with an increase during stationary and degeneration phases of the culture. These data suggest that fluidity parameters could be used to follow the behaviour of hybridoma cell cultures.
INSERM U 284, Vandoeuvre, France.
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V
Eyl
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Muller
M
M
Donner
M
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Maugras
J F
JF
Stoltz
D002462:Cell Membrane / Q000502:physiology; D003470:Culture Media; D004161:Diphenylhexatriene / Q000031:analogs & derivatives; D005454:Fluorescence Polarization; D005456:Fluorescent Dyes; D006801:Humans; D006825:Hybridomas / Q000166:cytology / Q000502:physiology*; D007700:Kinetics; D008560:Membrane Fluidity
D003470:Culture Media; D005456:Fluorescent Dyes; D004161:Diphenylhexatriene; C033628:1-(4-(trimethylamino)phenyl)-6-phenylhexa-1,3,5-triene