A new human fusion partner, HK-128, for making human-human hybridomas producing monoclonal IgG antibodies.
A hypoxanthine-aminopterin-thymidine (HAT) sensitive human fusion partner cell line, HK-128 was established from a human plasmacytoma line, LICR-LON-HMy2 (HMy2). The HK-128 cells showed a 100% cloning efficiency. Fusion efficiency of HK-128 was so high that one hybridoma cell was produced by fusion of 10(5) cells of HK-128 with lymphocytes, obtained from lymph nodes of breast cancer patients. About 90% of the resulted hybridomas were IgG producers. The remainder revealed IgM producing activity, which was lost by long term culture. This result indicates that the HK-128 cell line has an advantage for making hybridoma cells producing IgG. Among ca. 7,000 hybridomas obtained by fusion of HK-128 with lymphocytes of a breast cancer patient, we could establish a hybridoma cell line which produced IgG specifically reacting to a human breast cancer cell line, MCF-7.
Kawahara
H
H
Department of Food Science and Technology, Faculty of Agriculture, Kyushu University, Fukuoka, Japan.
Yamada
K
K
Shirahata
S
S
Murakami
H
H
D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D000951:Antigens, Neoplasm / Q000032:analysis; D001943:Breast Neoplasms / Q000276:immunology; D002459:Cell Fusion; D002469:Cell Separation; D006801:Humans; D006825:Hybridomas / Q000276:immunology*; D007074:Immunoglobulin G / Q000096:biosynthesis; D007132:Immunoglobulin Isotypes / Q000378:metabolism; D010954:Plasmacytoma / Q000276:immunology; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D007074:Immunoglobulin G; D007132:Immunoglobulin Isotypes