Identification of the B cell derived T cell colony promoting activity to soluble CD23.
We previously reported that supernatants of phytohemagglutinin (PHA)-stimulated normal human B cells (NBCsup) contain a T cell colony promoting activity. NBCsup were able to (a) increase the number of secondary colonies generated under PHA and interleukin-2 (IL-2) stimulation by peripheral blood-derived primary T colony cells, (b) enhance the ability of CD4+ but not CD8+ peripheral blood T cells to form agar colonies in the presence of PHA and IL-2 and (c) support in vitro differentiation of CD2-3-4-8- prothymocytes into CD2+3+4+ T cells. This activity was therefore refered to as Prothymocyte Differentiating Activity (PTDA). Subsequent studies pointed to striking biochemical and cell source homologies between this B cell derived factor and the 25-kDa soluble CD23 (sCD23). sCD23 has been recently found to display prothymocyte differentiating activity.
Centre de Recherches sur les Maladies du Sang, Hopital La Miletrie, Poitiers, France.
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F
Morel
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Goube de Laforest
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Brizard
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Gombert
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JC
Lecron
D001402:B-Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects; D017077:Culture Media, Conditioned / Q000494:pharmacology; D016207:Cytokines / Q000494:pharmacology; D006801:Humans; D007073:Immunoglobulin E / Q000494:pharmacology; D007376:Interleukin-2 / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects; D010835:Phytohemagglutinins; D017455:Receptors, IgE / Q000502:physiology*; D011993:Recombinant Fusion Proteins / Q000494:pharmacology
D017077:Culture Media, Conditioned; D016207:Cytokines; D007376:Interleukin-2; D010835:Phytohemagglutinins; D017455:Receptors, IgE; D011993:Recombinant Fusion Proteins; D007073:Immunoglobulin E