Identification and quantification of rodent malaria strains and species using gene probes.
A DNA probe PCsv4 and a subclone thereof PCsv4.1, hybridize specifically to rodent malaria DNA. DNA purified from a small volume (10 microliters) of infected mouse blood was used to determine the composition of the parasite population present. The hybridization signal following PCsv4 probing of slot-blotted DNA correlated directly with parasitaemia. The hybridization pattern and intensity, resulting from probing restriction enzyme digested and Southern-blotted genomic DNA, determined the identity of the infecting parasite line(s), and provided a semi-quantitative measure of parasite burden. Fifteen parasite lines representative of all four Plasmodium species infecting rodents can be differentiated in this way.
Snounou
G
G
Division of Parasitology, National Institute for Medical Research, Mill Hill, London.
Bourne
T
T
Jarra
W
W
Viriyakosol
S
S
Brown
K N
KN
D000818:Animals; D001345:Autoradiography; D015139:Blotting, Southern; D015342:DNA Probes; D016054:DNA, Protozoan / Q000032:analysis*; D015246:Deoxyribonuclease EcoRI; D004195:Disease Models, Animal; D008288:Malaria / Q000469:parasitology*; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D009693:Nucleic Acid Hybridization; D010961:Plasmodium / Q000145:classification* / Q000235:genetics; D010962:Plasmodium berghei / Q000145:classification / Q000235:genetics; D016788:Plasmodium chabaudi / Q000145:classification / Q000235:genetics; D010967:Plasmodium yoelii / Q000145:classification / Q000235:genetics; D012150:Polymorphism, Restriction Fragment Length; D012044:Regression Analysis; D015183:Restriction Mapping
D015342:DNA Probes; D016054:DNA, Protozoan; D015246:Deoxyribonuclease EcoRI