Isolation and characterization of genes encoding chaperonin 60 beta from Arabidopsis thaliana.
Chaperonins (Cpn) are implicated in the folding and assembly of multimeric proteins in plastids and mitochondria of eukaryotes and in prokaryotes. Plastid Cpn is composed of two different polypeptides termed Cpn60 alpha and Cpn60 beta. We have isolated cDNA and genomic clones encoding Cpn60 beta from Arabidopsis thaliana. The steady-state level of the cpn60 beta mRNAs is higher in etiolated leaves and sucrose-treated plants as compared to control leaves. The A. thaliana cpn60 beta gene family consists of at least three different coding units. It was confirmed that Cpn beta-encoding genes have a high level of conservation among plants.
Centro de Investigaciones Biológicas, FIBA, Mar del Plata, Argentina.
E
E
Zabaleta
A
A
Oropeza
B
B
Jiménez
G
G
Salerno
M
M
Crespi
L
L
Herrera-Estrella
D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D018833:Chaperonins; D005786:Gene Expression Regulation / Q000187:drug effects; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics*; D010944:Plants / Q000235:genetics*; D011506:Proteins / Q000235:genetics*; D015183:Restriction Mapping; D013395:Sucrose / Q000494:pharmacology
D010940:Plant Proteins; D011506:Proteins; D013395:Sucrose; D018833:Chaperonins