Quantitative flow measurements in bioreactors by nuclear magnetic resonance imaging.
We have developed nuclear magnetic resonance (NMR) flow imaging techniques to measure fluid flow in a cell-free hollow fiber bioreactor (HFBR). Using 1H NMR we track the motion of protons and obtain velocity distributions as a function of position and time. These measurements enable the visualization of flow patterns needed for module design and for establishing desired operating conditions. Uneven flow in the cell-containing region of an HFBR can result in concentration gradients and uneven cell distribution that may lead to reduced cell viability. Results from this non-invasive method could be used to design more efficient cell bioreactors or membrane separation devices.
Intermagnetics General Corporation, Troy, NY 12180.
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Hammer
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Heath
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Mirer
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Belfort
D001709:Biotechnology / Q000295:instrumentation*; D002138:Calibration; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*