Detection of tick-borne encephalitis virus in ixodid ticks collected in natural foci by time-resolved fluoroimmunoassay.
Time-resolved fluoroimmunoassay (TR-FIA) was used for the first time for evaluation of infestation of ixodid ticks with tick-borne encephalitis virus. Comparison of TR-FIA results with those obtained in enzyme immunoassay and by virus isolation confirmed the high efficacy of the method in question. Positive results of TR-FIA coincided with the data of virus isolation in 83.6% cases, the level of false-negative results did not exceed 1.2%, the overall time consumption amounted to about 1.2 hr.
Ivanovsky Institute of Virology of the U.S.S.R. AMS, Moscow.
V G
VG
Pomelova
N A
NA
Lavrova
S Y
SY
Gaidamovich
O V
OV
Navolokin
P
P
Halonen
M V
MV
Sokolova
I G
IG
Kharitonenkov
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D004669:Encephalitis Viruses, Tick-Borne / Q000276:immunology*; D004675:Encephalitis, Tick-Borne / Q000175:diagnosis*; D015200:Fluoroimmunoassay / Q000379:methods*; D007124:Immunoenzyme Techniques; D013987:Ticks / Q000382:microbiology*
D000956:Antigens, Viral