Absence of infectious human immunodeficiency virus type 1 in "natural" eccrine sweat.
Although human immunodeficiency virus type 1 (HIV-1) has been found in numerous body fluids, there are no reports of attempts to demonstrate this virus in eccrine sweat, a fluid frequently encountered during person-to-person interactions. "Natural" eccrine sweat samples and blood from 50 HIV-1-seropositive patients and 2 HIV-1-seronegative controls were cultured for HIV-1 by a cocultivation method. Polymerase chain reaction for HIV-1 RNA and proviral DNA was done on 40 sweat samples (39 patients, 1 control). HIV-1 was isolated from peripheral blood mononuclear cells of 39 (78%) of 50 patients but from none of 52 sweat samples. No HIV-1 viral DNA or RNA was detected in the 40 sweat samples tested. With present methodology, infectious HIV-1 cannot be demonstrated in "natural" eccrine sweat samples from HIV-infected patients.
Division of Infectious Diseases, New York Medical College, Westchester County Medical Center, Valhalla 10595.
G P
GP
Wormser
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S
Bittker
G
G
Forseter
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IK
Hewlett
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I
Argani
B
B
Joshi
J S
JS
Epstein
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D
Bucher
D000163:Acquired Immunodeficiency Syndrome / Q000382:microbiology; D015496:CD4-Positive T-Lymphocytes; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D015658:HIV Infections / Q000382:microbiology*; D015497:HIV-1 / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D007958:Leukocyte Count; D007963:Leukocytes, Mononuclear / Q000382:microbiology; D008297:Male; D016133:Polymerase Chain Reaction; D011533:Proviruses / Q000235:genetics / Q000302:isolation & purification; D012367:RNA, Viral / Q000032:analysis; D012307:Risk Factors; D013542:Sweat / Q000382:microbiology*
D004279:DNA, Viral; D012367:RNA, Viral