In situ characterization of mononuclear cells in marginal periodontitis of patients with Down's syndrome.
An indirect immunofluorescence technique on cryostat sections was used to study the cellular composition in chronic marginal periodontitis (CMP) of patients with Down's syndrome (DS). The findings were compared with CMP lesions in otherwise normal patients (NP). The distribution and amount of CD22+ cells (B lymphocytes), CD3+ cells (pan T lymphocytes), CD4+ cells (helper T subset), CD8+ cells (suppressor/cytotoxic T subset), and CD11c+ cells (in tissue, mainly monocytes and macrophages) were investigated. Morphologic studies showed a denser inflammatory infiltrate in DS than in NP. Countings showed significant differences in cell distribution (p = 0.0003) and cell profiles (p = 0.0273) between the two groups. The median CD4+/CD8+ ratio in DS (2.73) was significantly higher (p = 0.0024) than found in gingival inflammatory lesions from NP (1.08). The present study shows that DS patients have a different, more pronounced, immune response in CMP than NP.
Søhoel
P D
PD
Department of Periodontology, School of Dentistry, University of Bergen, Norway.
Johannessen
A C
AC
Kristoffersen
T
T
Haugstvedt
Y
Y
Nilsen
R
R
D000328:Adult; D000368:Aged; D001402:B-Lymphocytes / Q000473:pathology; D015496:CD4-Positive T-Lymphocytes / Q000473:pathology; D002908:Chronic Disease; D003238:Connective Tissue / Q000473:pathology; D004314:Down Syndrome; D004848:Epithelium / Q000473:pathology; D005260:Female; D005881:Gingiva / Q000473:pathology; D006801:Humans; D007958:Leukocyte Count; D008214:Lymphocytes / Q000473:pathology*; D008264:Macrophages / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000473:pathology*; D010518:Periodontitis / Q000473:pathology*; D013601:T-Lymphocytes / Q000473:pathology; D013602:T-Lymphocytes, Cytotoxic / Q000473:pathology