Comparison of CD composition produced by chimeric CGTases.
Using 12 chimeric cyclomaltodextrin glucanotransferases (CGTases) constructed from two genes, the CGTase gene from the alkalophilic Bacillus sp. strain No. 38-2 (CGTase 38-2 gene) and the CGTase gene from the alkalophilic Bacillus sp. strain No. 17-1 (CGTase 17-1 gene), we compared the effect of those chimeric enzymes on cyclodextrin (CD) production, especially on the composition of the CDs produced. It was found that the N-terminal and the C-terminal segments were important for CD production. Chimeric enzymes that contained the N-terminal and the C-terminal segments derived from CGTase 38-2 produced large amounts of CD, and especially a higher proportion of alpha-CD than those of other chimeric enzymes.
Riken Institute, Saitama, Japan.
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Kaneko
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Kudo
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Horikoshi
D001407:Bacillus / Q000201:enzymology / Q000235:genetics*; D002678:Chimera; D003505:Cyclodextrins / Q000096:biosynthesis*; D003912:Dextrins / Q000096:biosynthesis*; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D005798:Genes, Bacterial; D005964:Glucosyltransferases / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D007527:Isoenzymes / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D007700:Kinetics; D010957:Plasmids; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D013213:Starch / Q000096:biosynthesis*
D003505:Cyclodextrins; D003912:Dextrins; D007527:Isoenzymes; D013213:Starch; D005964:Glucosyltransferases; C019963:cyclomaltodextrin glucanotransferase