N-methyl-N'-nitro-N-nitrosoguanidine-induced light emission in Chinese hamster cell cultures: correlation with enhancement of chromosomal aberrations.
N-Methyl-N'-nitro-N-nitrosoguanidine (MNNG) was found to induce an ultraweak photon emission in cultures of Chinese hamster fibroblasts (CHL). Measurements suggest that the light emission is due to a reaction between MNNG and cellular metabolites. The light emission depended on the concentration of MNNG and was oxygen-dependent, disappearing in a nitrogen atmosphere. Superoxide dismutase (SOD) or sodium azide decreased the emission intensity. The production of chromosomal aberrations in CHL by MNNG was correlated with the light emission intensity and was inhibited in the presence of SOD.
Biophoton Project, Research Development Corporation of Japan JRDC, Sendai.
M
M
Kimura
P
P
Roschger
M
M
Kobayashi
S
S
Kimura
H
H
Inaba
D000818:Animals; D002460:Cell Line; D002869:Chromosome Aberrations; D006224:Cricetinae; D003412:Cricetulus; D005347:Fibroblasts / Q000187:drug effects; D007700:Kinetics; D008027:Light; D008769:Methylnitronitrosoguanidine / Q000494:pharmacology*; D009152:Mutagenicity Tests; D009153:Mutagens / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000494:pharmacology; D013997:Time Factors
D009153:Mutagens; D008769:Methylnitronitrosoguanidine; D013482:Superoxide Dismutase