Use of ars18 based vectors to increase protein production in Yarrowia lipolytica.
The isolation of ars sequence from the yeast Yarrowia lipolytica has recently been reported (Fournier et al., 1991). Vectors containing ars18 have been used to increase homologous and heterologous protein production. Examples presented are the Yarrowia lipolytica alkaline extracellular protease (AEP), the porcine alpha 1-interferon and the bovine prochymosin. A 2- to 6-fold increase in the corresponding protein production was observed and in several cases it was established that it corresponded to the copy number of plasmid in the cell.
Laboratoire de Génétique des Microorganismes, Institut National Agronomique Paris-Grignon, Thiverval-Grignon, France.
J M
JM
Nicaud
P
P
Fournier
C
C
La Bonnardière
M
M
Chasles
C
C
Gaillardin
D000818:Animals; D002417:Cattle; D012085:Chymosin / Q000096:biosynthesis*; D010450:Endopeptidases / Q000096:biosynthesis*; D004792:Enzyme Precursors / Q000096:biosynthesis*; D005784:Gene Amplification; D005800:Genes, Fungal; D005822:Genetic Vectors; D007370:Interferon Type I / Q000096:biosynthesis*; D010957:Plasmids; D013552:Swine; D015003:Yeasts / Q000235:genetics*
D004792:Enzyme Precursors; D007370:Interferon Type I; D010450:Endopeptidases; C032443:prorennin; D012085:Chymosin