Comparison of the N-linked oligosaccharide structures of the two major human myelin glycoproteins MAG and P0: assessment of the structures bearing the epitope for HNK-1 and human monoclonal immunoglobulin M found in demyelinating neuropathy.
The epitope for HNK-1 and patient's monoclonal autoantibodies in demyelinating polyneuropathy associated with immunoglobulin M gammopathy is borne by different types of N-linked oligosaccharide structures in human P0 and myelin-associated glycoprotein (MAG). Fourteen glycopeptide fractions bearing different oligosaccharide structures were obtained from either MAG or P0 glycopeptides by serial lectin affinity chromatography on concanavalin A-Sepharose, Phaseolus vulgaris erythrophytohemagglutinin-agarose, Pisum sativum agglutinin-agarose, and Phaseolus vulgaris leucophytohemagglutinin-agarose. As shown by dot-TLC plate immunostaining, the same MAG and P0 glycopeptide fractions were recognized by HNK-1 and patient's immunoglobulin M, confirming that these antibodies display similar specificities. The antigenic carbohydrate was present in glycopeptide fractions that either interact with Pisum sativum agglutinin-agarose or were bound by Aleuria aurantia agglutinin-digoxigenin, indicating that these structures contained alpha(1-6)fucose residues. This study demonstrates that the L2/HNK-1 epitope is borne mainly or even exclusively by N-linked oligosaccharide structures alpha(1-6)fucosylated in the core.
Burger
D
D
Department of Neurology, Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland.
Perruisseau
G
G
Simon
M
M
Steck
A J
AJ
D000911:Antibodies, Monoclonal / Q000032:analysis; D000943:Antigens, Differentiation / Q000276:immunology*; D018959:CD57 Antigens; D005591:Chemical Fractionation; D002846:Chromatography, Affinity; D003711:Demyelinating Diseases / Q000276:immunology / Q000378:metabolism*; D000939:Epitopes; D006801:Humans; D007075:Immunoglobulin M / Q000032:analysis*; D037102:Lectins; D018993:Myelin P0 Protein; D009185:Myelin Proteins / Q000737:chemistry* / Q000276:immunology; D018992:Myelin-Associated Glycoprotein; D009844:Oligosaccharides / Q000737:chemistry* / Q000276:immunology
D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation; D018959:CD57 Antigens; D000939:Epitopes; D007075:Immunoglobulin M; D037102:Lectins; D018993:Myelin P0 Protein; D009185:Myelin Proteins; D018992:Myelin-Associated Glycoprotein; D009844:Oligosaccharides