Morphology, DNA ploidy and allele losses on chromosome 11 in sporadic hyperparathyroidism and that associated with multiple neoplasia, type 1.
OBJECTIVE
To analyse different forms of hyperparathyroidism (for example, sporadic and multiple endocrine neoplasia type 1 (MEN 1)) by histopathology, DNA cytometry and by the presence of allele losses on chromosome 11, thereby identifying common characteristics.


METHODS
Enlarged glands from 26 patients with hyperparathyroidism (23 sporadic and 3 MEN 1). Cytometric assessment was made of 28 glands.


RESULTS
Nine patients had multiple gland disease and 15 had single gland disease (14 sporadic and 1 MEN 1). DNA cytometry showed that 18 (15 sporadic and all 3 MEN 1) were diploid, seven tetraploid, and two aneuploid. Two glands from one sporadic case showed different ploidy patterns. Seven patients with sporadic and all three with MEN 1 hyperparathyroidism had allele losses for chromosome 11 in the analysed glands.


CONCLUSIONS
There were no significant differences in histopathological appearances, ploidy, or allele losses among abnormal glands from a variety of forms of hyperparathyroidism.
Department of Surgery, Karolinska Hospital, Stockholm, Sweden.
K
K
Sandelin
C
C
Larsson
U G
UG
Falkmer
L O
LO
Farnebo
L
L
Grimelius
M
M
Nordenskjöld
D000328:Adult; D000368:Aged; D000483:Alleles; D002880:Chromosomes, Human, Pair 11 / Q000648:ultrastructure*; D004247:DNA / Q000032:analysis* / Q000235:genetics; D003937:Diagnosis, Differential; D005260:Female; D005434:Flow Cytometry / Q000379:methods; D005819:Genetic Markers; D005838:Genotype; D006801:Humans; D006961:Hyperparathyroidism / Q000235:genetics / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D009377:Multiple Endocrine Neoplasia / Q000235:genetics / Q000473:pathology*; D010280:Parathyroid Glands / Q000473:pathology; D010282:Parathyroid Neoplasms / Q000235:genetics / Q000473:pathology*; D011003:Ploidies
D005819:Genetic Markers; D004247:DNA