Biosynthesis of inter-alpha-trypsin inhibitor and alpha 1-microglobulin in a human hepatoma cell line.
1. Biosynthesis of alpha 1-microglobulin and inter-alpha-trypsin inhibitor was investigated in a human hepatoma cell line HepG-2. 2. alpha 1-Microglobulin was translated as a precursor common with the light chain of inter-alpha-trypsin inhibitor. 3. alpha 1-Microglobulin was synthesized and secreted into the growth medium within 30 min. 4. Processing of inter-alpha-trypsin-inhibitor-related proteins appeared slow and incomplete. The light chain was connected via a chondroitinsulphate to a heavy chain to form a 125,000-Mr protein and secreted within 1-4 hr.
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Department of Clinical Chemistry, Rigshospitalet, University of Copenhagen, Denmark.
D000510:Alpha-Globulins / Q000096:biosynthesis* / Q000737:chemistry; D050260:Carbohydrate Metabolism; D002241:Carbohydrates / Q000737:chemistry; D006528:Carcinoma, Hepatocellular; D002738:Chloroquine / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008113:Liver Neoplasms; D011233:Precipitin Tests; D011499:Protein Processing, Post-Translational; D014361:Trypsin Inhibitors / Q000096:biosynthesis* / Q000737:chemistry; D014407:Tumor Cells, Cultured
D000510:Alpha-Globulins; D002241:Carbohydrates; D014361:Trypsin Inhibitors; C016001:alpha-1-microglobulin; C011877:inter-alpha-inhibitor; D002738:Chloroquine