Rapid and simultaneous assay of monoamine neurotransmitters and their metabolites in discrete brain areas of mice by HPLC with coulometric detection.
For simultaneous assay of the three monoamine neurotransmitters, norepinephrine, dopamine, and serotonin, and four respective metabolites in brain tissue, a rapid and simple method using high-performance liquid chromatography with coulometric detection is described. Because the present method permits the determination of these target substrates within 10 min or less in one chromatographic run, 150 samples can be analyzed using an autosampler and an integrator in a 24-h period. Within-run coefficients of variation for the target substrates in the standard solution and the whole brain sample were less than 3% and 2% (n = 40), respectively. The quantitative detection limits were 0.01-0.1 pmol. The present procedure was applied to measure the target substrates in several discrete brain areas in mice.
Department of Pharmacology, School of Dentistry, Iwate Medical University, Morioka, Japan.
H
H
Saito
S
S
Murai
E
E
Abe
Y
Y
Masuda
T
T
Itoh
D000818:Animals; D015306:Biogenic Monoamines / Q000378:metabolism*; D001923:Brain Chemistry / Q000502:physiology*; D002851:Chromatography, High Pressure Liquid; D004563:Electrochemistry; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D018377:Neurotransmitter Agents / Q000378:metabolism*
D015306:Biogenic Monoamines; D018377:Neurotransmitter Agents