Exponential amplification of nucleic acids: new diagnostics using DNA polymerases and RNA replicases.
Recent developments in DNA and RNA amplification technology are enabling the design of ultra-sensitive diagnostic assays for infectious diseases. The leading amplification technology is the polymerase chain reaction (PCR). An alternative approach, Q-beta amplification, also promises remarkable speed and precise quantification of assay results.
Centro de Investigación sobre Ingenieria Genética y Biotecnologia, UNAM, F.R.
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Lizardi
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Kramer
D001483:Base Sequence; D003141:Communicable Diseases / Q000175:diagnosis*; D004259:DNA-Directed DNA Polymerase; D005189:False Positive Reactions; D006801:Humans; D008969:Molecular Sequence Data; D021141:Nucleic Acid Amplification Techniques; D009696:Nucleic Acids / Q000378:metabolism*; D011777:Q beta Replicase; D012367:RNA, Viral / Q000235:genetics; D012324:RNA-Dependent RNA Polymerase
D009696:Nucleic Acids; D012367:RNA, Viral; D011777:Q beta Replicase; D012324:RNA-Dependent RNA Polymerase; D004259:DNA-Directed DNA Polymerase