Immunoprecipitation of a pertussis toxin substrate of the G(o) family from rat islets of Langerhans.
Rat islets express a pertussis toxin sensitive G-protein involved in receptor-mediated inhibition of insulin secretion. This has been assumed previously to represent "G(i)" which couples inhibitory receptors to adenylate cyclase. Incubation of islet G-proteins with 32P-NAD and pertussis toxin resulted in the labelling of a band of molecular weight 40,000. This band was very broad and did not allow resolution of individual components. Incubation of the radiolabelled proteins with an anti-G(o) antiserum resulted in specific immunoprecipitation of a 32P-labelled band. These results demonstrate that the complement of pertussis toxin sensitive G-proteins in rat islets includes G(o).
Berrow
N S
NS
Department of Biological Sciences, Keele University, Staffs, UK.
Hurst
R D
RD
Chan
S L
SL
Morgan
N G
NG
D037361:Adenylate Cyclase Toxin; D000262:Adenylyl Cyclases / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D002478:Cells, Cultured; D004837:Epinephrine / Q000494:pharmacology; D019204:GTP-Binding Proteins / Q000032:analysis* / Q000276:immunology; D007515:Islets of Langerhans / Q000378:metabolism*; D008969:Molecular Sequence Data; D009638:Norepinephrine / Q000494:pharmacology; D037342:Pertussis Toxin; D011233:Precipitin Tests; D051381:Rats; D017208:Rats, Wistar; D013004:Somatostatin / Q000494:pharmacology; D010566:Virulence Factors, Bordetella / Q000378:metabolism* / Q000494:pharmacology
D037361:Adenylate Cyclase Toxin; D010566:Virulence Factors, Bordetella; D013004:Somatostatin; D037342:Pertussis Toxin; D019204:GTP-Binding Proteins; D000262:Adenylyl Cyclases; D009638:Norepinephrine; D004837:Epinephrine