Termination of second messenger signaling in olfaction.
By using isolated rat olfactory cilia and a fast kinetics methodology, it has been demonstrated that odorant-induced second messenger signaling in the millisecond time range is terminated via phosphorylation reactions catalyzed by specific protein kinases. The cyclic adenosine nucleotide pathway is turned off by kinase A activity, whereas the inositol trisphosphate cascade is terminated by kinase C. The data support the concept that desensitization of odorant responses involves phosphorylation of key elements in the transduction cascade.
Boekhoff
I
I
University Stuttgart-Hohenheim, Institute of Zoophysiology, Stuttgart, Federal Republic of Germany.
Breer
H
H
D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D000818:Animals; D000242:Cyclic AMP / Q000502:physiology; D004988:Ethers, Cyclic / Q000494:pharmacology; D005246:Feedback; D015544:Inositol 1,4,5-Trisphosphate / Q000378:metabolism; D009570:Nitriles; D019319:Okadaic Acid; D009831:Olfactory Mucosa / Q000502:physiology*; D015240:Phorbol 12,13-Dibutyrate / Q000494:pharmacology; D011493:Protein Kinase C / Q000037:antagonists & inhibitors; D011494:Protein Kinases / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D015290:Second Messenger Systems / Q000502:physiology*; D015398:Signal Transduction; D012903:Smell / Q000502:physiology*; D013110:Sphingosine / Q000494:pharmacology; D013997:Time Factors
D004988:Ethers, Cyclic; D009570:Nitriles; D019319:Okadaic Acid; D015240:Phorbol 12,13-Dibutyrate; C054764:citralva; D015544:Inositol 1,4,5-Trisphosphate; D000242:Cyclic AMP; D011494:Protein Kinases; D011493:Protein Kinase C; D013110:Sphingosine; D015056:1-Methyl-3-isobutylxanthine