Single pass sequencing and physical and genetic mapping of human brain cDNAs.
We have performed single pass sequencing of 1,024 human brain cDNAs, over 900 of which seem to represent new human genes. Library prescreening with total brain cDNA significantly reduced repeated sequencing of highly represented cDNAs. A subset of sequenced cDNAs were physically mapped to their chromosomal locations using gene-specific STS primers derived from 3' untranslated regions. We have also determined that human brain cDNAs represent a rich source of gene-associated polymorphic markers. Microsatellite-containing cDNAs can be physically mapped and converted to highly informative genetic markers, thus facilitating integration of the human physical, expression and genetic maps.
Khan
A S
AS
Department of Pharmacology, University of Colorado Health Sciences Center, Denver 80262.
Wilcox
A S
AS
Polymeropoulos
M H
MH
Hopkins
J A
JA
Stevens
T J
TJ
Robinson
M
M
Orpana
A K
AK
Sikela
J M
JM
D001483:Base Sequence; D001923:Brain Chemistry / Q000235:genetics*; D002675:Child, Preschool; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D004276:DNA, Satellite / Q000032:analysis; D016208:Databases, Factual; D005260:Female; D005333:Fetus; D015723:Gene Library; D006801:Humans; D007223:Infant; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D011506:Proteins / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics; D017422:Sequence Analysis, DNA / Q000379:methods; D012689:Sequence Homology, Nucleic Acid; D014897:Spinal Muscular Atrophies of Childhood / Q000235:genetics
D018076:DNA, Complementary; D004276:DNA, Satellite; D011506:Proteins