Estrogen induction of cytochrome c oxidase subunit III in rat hippocampus.
Differential screening of a cDNA library prepared from mRNA of the hippocampus of estrogen-stimulated ovariectomized female rats led to the identification of a single estrogen-induced clone. Analysis of the sequence identified this cDNA as the gene coding for subunit III of the enzyme cytochrome c oxidase. Cytochrome c oxidase subunit III mRNA levels significantly increased as early as 3 h following the administration of a single dose of hormone. This effect was visible in the hippocampus and in the hypothalamus, but not in the other brain areas examined. Because subunit III of the cytochrome c oxidase is of mitochondrial origin, the mechanism involved in the estrogenic effect is still unknown. The observation that the activity of cytochrome c oxidase can also be induced by estrogens in the hippocampus indicates that this induction may be secondary to the increased expression of the other subunits of cytochrome c oxidase or to the general increase of neuronal activity.
Milano Molecular Pharmacology Laboratory, University of Milan, Italy.
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Bettini
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Maggi
D000595:Amino Acid Sequence; D000818:Animals; D001345:Autoradiography; D001483:Base Sequence; D003576:Electron Transport Complex IV / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D004790:Enzyme Induction; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D006624:Hippocampus / Q000201:enzymology; D008969:Molecular Sequence Data; D012313:RNA / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
D004958:Estradiol; D012313:RNA; D003576:Electron Transport Complex IV