Commercial levels of chymosin production by Aspergillus.
We have increased the production of bovine chymosin in Aspergillus niger var. awamori to more than one gram per liter of secreted authentic enzyme by combining a mutagenesis protocol with a novel robotic screening program. Analysis of the superior chymosin producing strains indicated that they have enhanced capabilities to secrete extracellular proteins.
Dunn-Coleman
N S
NS
Genencor International Inc., South San Francisco, CA 94080.
Bloebaum
P
P
Berka
R M
RM
Bodie
E
E
Robinson
N
N
Armstrong
G
G
Ward
M
M
Przetak
M
M
Carter
G L
GL
LaCost
R
R
D001234:Aspergillus niger / Q000187:drug effects / Q000235:genetics*; D001709:Biotechnology / Q000295:instrumentation / Q000379:methods; D012085:Chymosin / Q000096:biosynthesis* / Q000235:genetics; D003847:Deoxyglucose / Q000494:pharmacology; D004352:Drug Resistance, Microbial / Q000235:genetics; D016296:Mutagenesis; D009492:Neurospora crassa / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis
D011994:Recombinant Proteins; D003847:Deoxyglucose; D012085:Chymosin