High level of HTLV-I specific protein expression in a patient with adult T-cell leukemia, chronic progressive myelopathy and Kaposi's sarcoma.
Analysis was made of serum anti-HTLV-I antibodies, virus-specific proteins in peripheral blood lymphocytes (PBL) and proviruses in lymphocyte DNA of a patient with adult T-cell leukemia (ATL), Kaposi's sarcoma, and chronic myelopathy. Using Western blot and PCR (with HIV-1 specific primers), it was shown that Kaposi's sarcoma was not linked to HIV infection. Western blot analysis of serum revealed antibodies against p19, p24 and Pr 53 of HTLV-I. Examination of proteins in fresh PBL by Western blot revealed a high level of HTLV-I specific protein expression. Southern blot analysis of the patient's DNA revealed two different sites for HTLV-I provirus integration.
UPR A0043 CNRS Rétrovirus et Rétrotransposons des Vertébrés, Hôpital Saint-Louis, Paris, France.
V A
VA
Morozov
S
S
Lagaye
F
F
Saal
A
A
Bazarbachi
O
O
Gout
O
O
Lyon-Caen
J
J
Périés
D000368:Aged; D015139:Blotting, Southern; D004279:DNA, Viral / Q000032:analysis; D015480:Deltaretrovirus Antibodies / Q000032:analysis; D005260:Female; D015368:Human T-lymphotropic virus 1 / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D015458:Leukemia, T-Cell / Q000382:microbiology*; D015493:Paraparesis, Tropical Spastic / Q000382:microbiology*; D011533:Proviruses / Q000235:genetics; D015183:Restriction Mapping; D012514:Sarcoma, Kaposi / Q000382:microbiology*; D014764:Viral Proteins / Q000378:metabolism*
D004279:DNA, Viral; D015480:Deltaretrovirus Antibodies; D014764:Viral Proteins