Philanthotoxins block glutamatergic transmission in rat hippocampus--I. Reduction of high affinity glutamate uptake demonstrated by light microscopy surface autoradiography.
1. Effects of structural philanthotoxin (PTX) analogues on the high affinity uptake of [3H]-L-glutamate by slices of the rat hippocampus were studied using light microscopy surface autoradiography. 2. Considering the various effects of the PTX-analogues it can be concluded that the presence of an aromatic nucleus in the molecule is an important moiety, while the number and distribution of positive charges in the polyamine chain seems to be of a still not understood importancy.
Schluter
N C
NC
Department of Pharmacology, University of Amsterdam, The Netherlands.
Van Weeren-Kramer
J
J
Piek
T
T
Van Marle
J
J
D000818:Animals; D001224:Aspartic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D001345:Autoradiography / Q000379:methods; D018691:Excitatory Amino Acid Antagonists; D005260:Female; D005971:Glutamates / Q000378:metabolism*; D018698:Glutamic Acid; D006624:Hippocampus / Q000187:drug effects* / Q000378:metabolism; D066298:In Vitro Techniques; D008853:Microscopy; D015394:Molecular Structure; D011073:Polyamines; D051381:Rats; D011919:Rats, Inbred Strains; D009435:Synaptic Transmission / Q000187:drug effects*; D014862:Wasp Venoms / Q000494:pharmacology*
D018691:Excitatory Amino Acid Antagonists; D005971:Glutamates; D011073:Polyamines; D014862:Wasp Venoms; C011274:3-hydroxyaspartic acid; D001224:Aspartic Acid; D018698:Glutamic Acid; C034092:delta-philanthotoxin