Thy-1 antigen-mediated adhesion of mouse lymphoid cells to stromal cells of haemopoietic origin.
Thy-1 antigen is one of the widespread cell surface antigens. The antigen is expressed in the cells of a wide variety of different tissues and species and its pattern varies considerably during development and among species. Although the Thy-1 is one of the members of Ig-superfamily its function(s) remain unknown. Here we show that monoclonal antibodies to mouse Thy-1.2 antigen prevent adhesion of Thy-1.2-positive mouse T-leukemic cells 127 to monolayers of two mouse bone marrow stromal cell lines (14F1.1 and MBA-15). The same antibodies do not prevent adhesion of cell 127 to monolayers of two other mouse bone marrow stromal cell lines (MBA-2.1 and MBA-1.1.1) and to a wide spectrum of different normal and tumor mouse cell lines. A characteristic of the cell line 14F1.1 is the ability to support growth of mouse haemopoietic stem-cells. This data suggest that cells of stromal lines 14F1.1 and MBA-15 contain on their surface a specific ligand for the Thy-1 molecule. Cell-cell interaction via this ligand and the molecule on the cell surface may be an important step for development of certain lineages of cells.
Irlin
Y
Y
Department of Chemical Immunology, Weizmann Institute of Science, Rehovot, Israel.
Peled
A
A
D000818:Animals; D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D001853:Bone Marrow / Q000276:immunology; D001854:Bone Marrow Cells; D002448:Cell Adhesion / Q000276:immunology*; D002450:Cell Communication / Q000276:immunology; D002460:Cell Line; D008562:Membrane Glycoproteins; D051379:Mice; D015398:Signal Transduction / Q000276:immunology; D013601:T-Lymphocytes / Q000166:cytology* / Q000276:immunology*; D018800:Thy-1 Antigens
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D008562:Membrane Glycoproteins; D018800:Thy-1 Antigens