Isolation and characterization of type 1 fimbriae from a chicken pathogenic Escherichia coli serotype O78.
Type 1 fimbriae from chicken pathogenic Escherichia coli strain PDI-386 (serotype O78) was purified and characterized. Because of the acid-induced autoagglutination (T. Sekizaki, Y. Nakasato, and I. Nonomura, J. Vet. Med. Sci. 54, 493-499, 1992), the fimbriae could be easily purified by repeating acid sedimentation, washing, and dissolving in buffer (pH 8.0). In electron microscopy, the purified fimbriae showed a filament of 8 nm in diameter and 10 microns in average length. The molecular mass of the protein subunit of the purified fimbriae estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) was 19,000 daltons. The amino acid composition and its NH2-terminal sequence were similar to the previously described one of the Klebsiella pneumoniae type 1 fimbriae. Moreover, there was an immunological relatedness between them. These results indicated that a molecular diversity found between the fimbriae of E. coli and that of K. pneumoniae has already been existed among chicken pathogenic E. coli strains.
Sekizaki
T
T
National Institute of Animal Health, Ibaraki, Japan.
Miyazaki
S
S
Ito
H
H
Asawa
T
T
Nonomura
I
I
D000595:Amino Acid Sequence; D000818:Animals; D002645:Chickens / Q000382:microbiology*; D004926:Escherichia coli / Q000737:chemistry / Q000302:isolation & purification / Q000648:ultrastructure*; D004927:Escherichia coli Infections / Q000382:microbiology / Q000662:veterinary; D010861:Fimbriae, Bacterial / Q000737:chemistry*; D008969:Molecular Sequence Data; D011201:Poultry Diseases / Q000382:microbiology*