Improving purification of recombinant ribonuclease T1.
Purification of recombinant RNase T1 and its mutants has been improved by optimizing bacterial growth conditions, periplasmic fraction preparation and the use of a precolumn. The main part of the chromatographic separation could be automated due to the reproducibility of the procedure.
Landt
O
O
Abteilung Saenger, Institut für Kristallographie, Freie Universität Berlin, Germany.
Zirpel-Giesebrecht
M
M
Milde
A
A
Hahn
U
U
D001236:Aspergillus oryzae / Q000201:enzymology / Q000235:genetics; D001483:Base Sequence; D001709:Biotechnology; D002852:Chromatography, Ion Exchange; D004271:DNA, Fungal / Q000235:genetics; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D015202:Protein Engineering; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification; D006163:Ribonuclease T1 / Q000235:genetics / Q000302:isolation & purification*
D004271:DNA, Fungal; D011994:Recombinant Proteins; D006163:Ribonuclease T1