Establishment and characterization of a new human colon adenocarcinoma cell line: BCS-TC2.
A new cell line designated as BCS-TC2 was established in culture from a primary human colon adenocarcinoma. This cell line has been in continuous culture over a 36-month period. The cells grow as a monolayer sheet, displaying areas with a multilayered pattern as well as single cells and free-floating aggregates. The morphological, immunological, and ultrastructural features of these cells are in agreement with their epithelial origin. The characterization of this cell line indicated a 38 hr doubling time, and a colony forming efficiency of 2% in semisolid media and 22% in liquid culture, at low cell densities. These cells produce low amounts of carcinoembryonic antigen in culture (0.1 ng of CEA/10(6) cells). Sub-cutaneous injection into athymic mice shows that these cells have a non-tumorigenic capacity. Chromosomal analysis showed a karyotype 46 XX, -15, +der (15), inv (16) (p13::q13). BCS-TC2 cell line, which maintains in culture several characteristics of the original tumor, represents a useful model system for cell biology studies of primary and non-metastatic tumors.
Dpto. Bioquímica y Biología Molecular I, Universidad Complutense, Madrid, Spain.
J
J
Turnay
N
N
Olmo
J G
JG
Gavilanes
J
J
Benitez
M A
MA
Lizarbe
D000230:Adenocarcinoma / Q000235:genetics / Q000473:pathology; D000818:Animals; D002455:Cell Division; D003110:Colonic Neoplasms / Q000235:genetics / Q000473:pathology; D004848:Epithelium; D005260:Female; D006801:Humans; D007621:Karyotyping; D051379:Mice; D008807:Mice, Inbred BALB C; D008875:Middle Aged; D009368:Neoplasm Transplantation; D014407:Tumor Cells, Cultured