An inducible gene expression system for Neurospora crassa.
Neurospora crassa acetyl CoA synthetase is highly induced when the growing mycelium is transferred from sucrose- to acetate-based medium. The inducible promoter of this gene has been isolated and used to control the expression of glutamate dehydrogenase. Transformants containing this expression cassette show gdh levels up to 25 times higher than the nontransformed host strain. This expression cassette will form the basis of a system of heterologous gene expression.
Protein Engineering Department, AFRC Institute of Food Research, Shinfield, Reading, UK.
M E
ME
Collins
G
G
Briggs
C
C
Sawyer
P
P
Sheffield
I F
IF
Connerton
D000106:Acetate-CoA Ligase / Q000096:biosynthesis / Q000235:genetics*; D004271:DNA, Fungal / Q000235:genetics / Q000302:isolation & purification; D004790:Enzyme Induction; D015971:Gene Expression Regulation, Enzymologic; D015966:Gene Expression Regulation, Fungal; D005969:Glutamate Dehydrogenase / Q000235:genetics* / Q000378:metabolism; D009492:Neurospora crassa / Q000201:enzymology / Q000235:genetics*; D010957:Plasmids; D011401:Promoter Regions, Genetic
D004271:DNA, Fungal; D005969:Glutamate Dehydrogenase; D000106:Acetate-CoA Ligase