Development of a chromatographic process for large-scale purification of staphylococcal enterotoxin B.
A process for large-scale purification of staphylococcal enterotoxin B (SEB) is presented. The process consists of three chromatographic steps. The first two steps are based on ion-exchange chromatography and the final one on gel filtration. Diluted culture supernatant (2000 dm3) with a SEB content of 0.4 mg dm-3 were processed and purified. SEB was obtained in 1.5 dm3 of 0.1 mol dm-3 ammonium hydrogen carbonate buffer, pH 8.0. The final product produced a single band on SDS polyacrylamide gel electrophoresis and a single peak when subjected to analytical gel filtration. The overall recovery was 74%.
Pharmacia LKB Biotechnology AB, Uppsala, Sweden.
H J
HJ
Johansson
T N
TN
Pettersson
J H
JH
Berglöf
D002850:Chromatography, Gel / Q000379:methods; D002852:Chromatography, Ion Exchange / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D004768:Enterotoxins / Q000302:isolation & purification*; D005285:Fermentation; D013211:Staphylococcus aureus / Q000254:growth & development*
D004768:Enterotoxins; C031818:enterotoxin B, staphylococcal