The locus Om, responsible for the DDK syndrome, maps close to Sigje on mouse chromosome 11.
The DDK inbred strain of mouse has a striking particularity: when DDK females are crossed to males of other strains they exhibit a reduced fertility, whereas the reciprocal crosses (non-DDK females x DDK males) are fertile (Wakasugi et al. 1967; Wakasugi 1973). The low fertility results from an early embryonic lethality, the F1 embryos dying near the late morula-early blastocyst stage. Genetic analyses (Wakasugi 1974) and nuclear and cytoplasmic transfers (Renard and Babinet 1986; Babinet et al. 1990; Mann 1986), have shown that the failure of the embroys to develop is due to an incompatibility between a DDK maternally encoded cytoplasmic product and the non-DDK paternal genome. In order to elucidate the genetic determinism of this embryonic lethality, we have analyzed the fertility of male progeny from a backcross BALB/c females x (BALB/c x DDK)F1 males and that of males from a set of recombinant inbred (RI) strains, established from DDK and BALB/c progenitors, when mated with DDK females. Our results indicate that a single locus, Om, is responsible for the DDK syndrome and is located on Chromosome (Chr) 11, very close to the Sigje locus.
Unité de Génétique des Mammifères, Institut Pasteur, Paris, France.
P A
PA
Baldacci
V
V
Richoux
J P
JP
Renard
J L
JL
Guénet
C
C
Babinet
D000818:Animals; D015139:Blotting, Southern; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D004276:DNA, Satellite; D005260:Female; D005298:Fertility / Q000235:genetics*; D008040:Genetic Linkage; D005819:Genetic Markers; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008815:Mice, Inbred Strains; D012150:Polymorphism, Restriction Fragment Length; D013577:Syndrome
D004276:DNA, Satellite; D005819:Genetic Markers