Bone marrow processing with the Fresenius AS 104: initial results.
Prior to purging, cryopreservation, or ABO-incompatible bone marrow (BM) transplantation, we have concentrated 23 BM harvests using a modification of the "grancollect-protocol" and the recently available bone marrow stem cell (BMSC) protocol of the Fresenius AS 104 cell separator with the P1-Y set. Within 40-70 minutes, the initial marrow volume of 922 ml (+/- 408 ml) was processed two to three times. A mean of 59% (+/- 20%) of the initial mononuclear cells was recovered in a mean volume of 119 ml (+/- 31 ml). The recovery of clonogenic cells, measured by CFU-GM assays, was 98% (+/- 80%). Red blood cells in the BM concentrates were reduced to 8% (+/- 4%) of the initial number. The procedure was efficient and yielded a BM cell fraction suitable for purging, cryopreservation, and transplantation. All transplanted patients showed fast and sustained engraftments after autologous or allogeneic BM transplantation.
Abt. Innere Medizin und Poliklinik m.S. Hämatologie und Onkologie, Universitätsklinikum Rudolf Virchow, Freie Universität Berlin, FRG.
J
J
Zingsem
T
T
Zeiler
R
R
Zimmermann
V
V
Weisbach
H
H
Mitschulat
W
W
Siegert
R
R
Eckstein
D001854:Bone Marrow Cells; D002469:Cell Separation / Q000295:instrumentation*; D004906:Erythrocyte Count; D005069:Evaluation Studies as Topic; D006801:Humans; D007938:Leukemia / Q000473:pathology; D007958:Leukocyte Count; D008223:Lymphoma / Q000473:pathology; D012189:Retrospective Studies