Relationship of the flavodoxin isoforms from Porphyra umbilicalis.
The macroalga Porphyra umbilicalis contained two flavodoxins in approximately 5:1 ratio and differing in Mr by ca 1000. The N-terminal sequences of the isoforms were identical and there was strong immunochemical identity. Peptide mapping gave similar fragments which differed in Mr by constant amount for the two isoforms. The flavodoxins may therefore differ only at the C-terminus, possibly as a consequence of in vivo processing since inclusion of protease inhibitors during extraction had no effect on the ratio of the isoforms.
Department of Biochemistry, University College of Wales, Aberystwyth, Dyfed, U.K.
N T
NT
Price
A J
AJ
Smith
L J
LJ
Rogers
D000595:Amino Acid Sequence; D004591:Electrophoresis, Polyacrylamide Gel; D005418:Flavodoxin / Q000737:chemistry / Q000302:isolation & purification*; D007536:Isomerism; D008969:Molecular Sequence Data; D000461:Rhodophyta / Q000032:analysis*
D005418:Flavodoxin