Gene amplification in mammalian cells: strategies for protein production.
Gene amplification is an experimental strategy for increasing protein production in mammalian cells. Co-amplification of the target gene by genetically linking it to one or more selectable and amplifiable genetic markers is a particularly successful strategy. A number of papers published in the past year or two illustrate the use of gene amplification to achieve high levels of expression.
Kellems
R E
RE
Baylor College of Medicine, Houston, Texas.
D000818:Animals; D005784:Gene Amplification; D005819:Genetic Markers; D006801:Humans; D008322:Mammals; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics*
D005819:Genetic Markers; D011994:Recombinant Proteins