Colonization factor antigen CFA/IV (PCF8775) of human enterotoxigenic Escherichia coli: nucleotide sequence of the CS5 determinant.
Human enterotoxigenic Escherichia coli isolates expressing the colonization factor antigen CFA/IV (previously designated PCF8775) produce plasmid-encoded CS5 fimbriae. The nucleotide sequence of the region encoding the major CS5 fimbrial subunit was determined. The subunit is synthesized as a precursor of 203 amino acids (20.85 kDa) with a mature protein of 181 amino acids corresponding to a size of 18.6 kDa. The CS5 subunit shows homology to the corresponding component of porcine enterotoxigenic E. coli F41, particularly within the signal sequence and at the carboxy terminus.
Clark
C A
CA
Department of Microbiology and Immunology, University of Adelaide, Australia.
Heuzenroeder
M W
MW
Manning
P A
PA
D000595:Amino Acid Sequence; D000942:Antigens, Bacterial / Q000235:genetics*; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D000939:Epitopes / Q000032:analysis; D004926:Escherichia coli / Q000235:genetics*; D036781:Fimbriae Proteins; D010861:Fimbriae, Bacterial; D004854:Herpesvirus 4, Human; D006801:Humans; D008969:Molecular Sequence Data
D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D000939:Epitopes; C022924:colonization factor antigens; D036781:Fimbriae Proteins