Death of serum-free mouse embryo cells caused by transforming growth factor beta 1 and effects of nutritional factors.
Transforming growth factor beta 1 (1 ng/ml) caused death of serum-free mouse embryo cells cultured in a medium consisting of a 1:1 mixture of Dulbecco's Modified Eagle's medium and Ham's F12 medium supplemented with fibronectin, insulin, transferrin, epidermal growth factor, and high density lipoprotein. Cell death occurred in the presence of polyunsaturated fatty acids including linoleic acid in the absence of selenium. The death could be reversed by adding alpha-tocopherol to the culture indicating a mechanism involving fatty acid peroxidation. Butylated hydroxytoluene was a poor suppressor of cell death in contrast to alpha-tocopherol. High density lipoprotein and fatty acid-free albumin also suppressed cell death at the level of 20 micrograms/ml and 1 mg/ml, respectively. Transforming growth factor beta 1 also caused a low rate of cell growth after heat treatment of the cells at 45 degrees C.
Nutritional Biochemistry Laboratory, Kumamoto Women's University, Japan.
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D000418:Albumins / Q000494:pharmacology; D000818:Animals; D002084:Butylated Hydroxytoluene / Q000494:pharmacology; D016923:Cell Death / Q000187:drug effects; D016895:Culture Media, Serum-Free / Q000737:chemistry; D004622:Embryo, Mammalian / Q000166:cytology*; D005227:Fatty Acids / Q000494:pharmacology; D006358:Hot Temperature; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000494:pharmacology; D008075:Lipoproteins, HDL / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C / Q000196:embryology; D012643:Selenium / Q000494:pharmacology; D016212:Transforming Growth Factor beta / Q000494:pharmacology / Q000633:toxicity*; D014810:Vitamin E / Q000494:pharmacology
D000418:Albumins; D016895:Culture Media, Serum-Free; D005227:Fatty Acids; D008041:Linoleic Acids; D008075:Lipoproteins, HDL; D016212:Transforming Growth Factor beta; D014810:Vitamin E; D002084:Butylated Hydroxytoluene; D019787:Linoleic Acid; D012643:Selenium