Effects on rat renal osmolytes of extended treatment with an aldose reductase inhibitor.
1. The mammalian renal medulla uses sorbitol, myo-inositol, betaine and glycerophosphorylcholine as intracellular osmolytes. 2. Sorbitol synthesis was inhibited by feeding male Wistar rats the aldose reductase inhibitor sorbinil at 40 mg/kg/day for 71 d, and renal inner medullas were extracted for analysis. 3. Aldose reductase activities and sorbitol contents were greatly reduced in sorbinil-treated animals, while betaine contents increased significantly (with no other osmolytes changing). 4. The betaine increase compensated for the sorbitol decrease such that the total organic osmolytes maintained the same ratio to sodium contents as controls. 5. These results are identical to the pattern previously reported for sorbinil treatment of rats for 10 d, but not for 21 d.
Edmands
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Biology Department, Whitman College, Walla Walla, WA 99362.
Yancey
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D000449:Aldehyde Reductase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D000818:Animals; D001622:Betaine / Q000378:metabolism* / Q000494:pharmacology; D001835:Body Weight / Q000187:drug effects; D007093:Imidazoles / Q000494:pharmacology*; D048289:Imidazolidines; D007668:Kidney / Q000201:enzymology / Q000378:metabolism* / Q000502:physiology; D008297:Male; D009994:Osmolar Concentration; D051381:Rats; D017208:Rats, Wistar; D013012:Sorbitol / Q000378:metabolism*; D013997:Time Factors; D014556:Urine
D007093:Imidazoles; D048289:Imidazolidines; D001622:Betaine; D013012:Sorbitol; D000449:Aldehyde Reductase; C026411:sorbinil