Functional properties of acetylcholine receptors coexpressed with the 43K protein in heterologous cell systems.
The nicotinic acetylcholine (ACh) receptor is an integral membrane protein which mediates synaptic transmission at the skeletal neuromuscular junction. A key event in the development of the neuromuscular junction is the formation of high density aggregates of ACh receptors in the postsynaptic membrane. Receptor clustering has been attributed, in part, to their association with a peripheral membrane protein of Mr 43,000 (43K protein). We have addressed whether the association of the 43K protein can alter the single channel properties of the ACh receptor, and thus influence neuromuscular transmission at developing synapses, by expressing ACh receptors with and without the 43K protein in heterologous expression systems. We found that coexpression of the 43K protein with the receptor did not significantly alter either its single channel conductance or its mean channel open time. This was true in oocytes and also in COS cells where it was possible to localize 43K-induced clusters by fluorescence microscopy and to record from those clustered receptors. These data are in agreement with previous single channel studies which have shown that the properties of diffusely distributed and clustered receptors in native muscle cells from both mice and Xenopus do not differ.
Program in Molecular and Cellular Neurosciences, Dartmouth Medical School, Hanover, New Hampshire 03755-3833.
C
C
Brennan
P B
PB
Scotland
S C
SC
Froehner
L P
LP
Henderson
D000818:Animals; D002460:Cell Line; D002522:Chlorocebus aethiops; D015870:Gene Expression; D005822:Genetic Vectors; D066298:In Vitro Techniques; D007473:Ion Channels / Q000502:physiology; D008564:Membrane Potentials; D008970:Molecular Weight; D009124:Muscle Proteins / Q000235:genetics / Q000502:physiology*; D009865:Oocytes / Q000737:chemistry; D011950:Receptors, Cholinergic / Q000235:genetics / Q000502:physiology*; D014162:Transfection
D007473:Ion Channels; D009124:Muscle Proteins; D011950:Receptors, Cholinergic; C051609:peripheral membrane protein 43K