Distributions and colocalization of neuropeptide Y and somatostatin in the goldfish brain.
The distributions of single- and double-labelled neuropeptide Y- (NPY-) and somatostatin-immunoreactive (SOM-IR) perikarya and processes were determined in the goldfish brain using immunoperoxidase and immunofluorescence techniques, respectively. In double-labelled material, it was evident that although these two peptides showed markedly similar distributions, they were colocalized in very few instances. A high degree of colocalization of NPY and SOM was noted in the neurons of the ventrolateral telencephalon (VI), the entopenduncular nucleus (NE) and, to a lesser extent, in the dorsocentral nucleus of the telencephalon (Dc). In Vl and NE, neurons showing NPY-IR displayed SOM-IR and vice versa. The only other instance of colocalization was that noted in the brainstem, where SOM and NPY were colocalized in the large cell bodies of the medial column of the vagal motor complex. Single-labelled SOM- and NPY-IR neurons shared a very similar distribution in various nuclei in the diencephalon and in the optic tectum. Colocalization was also noted within fibers throughout many nuclei of the telencephalon and within fibers innervating the swim bladder, one of the peripheral organs to which neurons of the medial column of the vagal motor complex project. Processes in the torus semicircularis and vagal lobe showed single-labelled immunoreactivity for both SOM and NPY in distinct laminar patterns. Large single-labelled SOM-IR terminals appeared to form pericellular baskets in the eminentia granularis of the cerebellum. Single-labelled NPY- or SOM-IR fibers were also found in the secondary gustatory nucleus and tract, the facial lobe, descending trigeminal tract, reticular formation and spinal cord. As in mammalian species, select groups of neurons in teleosts colocalize the neuropeptides SOM and NPY.
Department of Anatomy, Faculty of Medicine, University of Ottawa, Ontario, Canada.
L C
LC
Pickavance
W A
WA
Staines
J N
JN
Fryer
D000818:Animals; D005075:Biological Evolution; D001921:Brain / Q000033:anatomy & histology*; D001923:Brain Chemistry; D005455:Fluorescent Antibody Technique; D006054:Goldfish / Q000502:physiology*; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D009478:Neuropeptide Y / Q000378:metabolism*; D012154:Reticular Formation / Q000033:anatomy & histology / Q000378:metabolism; D013004:Somatostatin / Q000378:metabolism*; D013116:Spinal Cord / Q000033:anatomy & histology / Q000378:metabolism
D009478:Neuropeptide Y; D013004:Somatostatin