Culture of neuronal cells from postnatal rat brain: application to the study of neurotrophic factors.
1. The authors developed a primary culture technique for neuronal cells from postnatal rat brains and studied the effects of neurotrophic factors on the naturally developed neurons. 2. We demonstrated changes in the neurotrophic role of nerve growth factor (NGF) during the developmental stages of the rat: NGF was shown to act as a differentiation factor in the early stages and as a survival factor later. 3. It appeared that interleukin-6 (IL-6) supported the survival of septal cholinergic neurons obtained from 10-day-old rats. IL-6, however, did not induce the differentiation of embryonic rat septal cholinergic neurons. IL-6 improved the survival of mesencephalic catecholaminergic neurons from postnatal and embryonic rat brains, which have known not to be response to NGF.
Division of Protein Biosynthesis, Osaka University, Japan.
Y
Y
Kushima
H
H
Hatanaka
D000818:Animals; D000831:Animals, Newborn; D001685:Biological Factors / Q000494:pharmacology*; D001921:Brain / Q000166:cytology* / Q000187:drug effects; D002470:Cell Survival; D002478:Cells, Cultured; D007150:Immunohistochemistry; D015850:Interleukin-6 / Q000494:pharmacology; D009414:Nerve Growth Factors / Q000494:pharmacology; D009474:Neurons / Q000166:cytology / Q000187:drug effects*; D051381:Rats; D014446:Tyrosine 3-Monooxygenase / Q000032:analysis
D001685:Biological Factors; D015850:Interleukin-6; D009414:Nerve Growth Factors; D014446:Tyrosine 3-Monooxygenase