Multidrug resistant HOB1 lymphoma cells express P-glycoprotein that does not play the major role in the development of drug resistance to adriamycin.
Two cell lines resistant to 0.1 microM vincristine (VCR) and 2.0 microM adriamycin (ADR), respectively, (designated HOB1/VCR0.1 and HOB1/ADR2.0) were established from a human immunoblastic B lymphoma cell line. These cell lines showed the typical MDR phenotype with overexpression of P-glycoprotein and decreased [3H]VCR accumulation. The retention amounts of intracellular [3H]VCR in these two cell lines could be augmented by verapamil. However, in spite of the overproduction of P-glycoprotein, both HOB1/VCR1.0 and HOB1/ADR2.0 cells did not exhibit decreased accumulation of intracellular [14C]ADR. And the retention of [14C]ADR was not affected by verapamil. Our data support that P-glycoprotein is a drug transporter more important for the development of drug resistance to VCR than to ADR.
Department of Biochemistry, National Defense Medical Center, Taipei, Taiwan, R.O.C.
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Kao
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D015152:Blotting, Northern; D002470:Cell Survival / Q000187:drug effects; D015342:DNA Probes; D004317:Doxorubicin / Q000378:metabolism* / Q000494:pharmacology*; D004351:Drug Resistance / Q000235:genetics*; D015870:Gene Expression; D006801:Humans; D007700:Kinetics; D008223:Lymphoma; D008562:Membrane Glycoproteins / Q000032:analysis / Q000235:genetics*; D012334:RNA, Neoplasm / Q000235:genetics / Q000302:isolation & purification; D015183:Restriction Mapping; D014407:Tumor Cells, Cultured; D014700:Verapamil / Q000494:pharmacology; D014750:Vincristine / Q000494:pharmacology
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D015342:DNA Probes; D008562:Membrane Glycoproteins; D012334:RNA, Neoplasm; D014750:Vincristine; D004317:Doxorubicin; D014700:Verapamil