Cloning and sequence analysis of cDNA encoding Rhizopus niveus lipase.
Complementary DNA encoding Rhizopus niveus lipase (RNL) was isolated from the R. niveus IF04759 cDNA library using a synthetic oligonucleotide corresponding to the amino acid sequence of the enzyme. A clone, which had an insert of 1.0 kilobase pairs, was found to contain the coding region of the enzyme. The lipase gene was expressed in Escherichia coli as a lacZ fusion protein. The mature RNL consisted of 297 amino acid residues with a molecular mass of 32 kDa. The RNL sequence showed significant overall homology to Rhizomucor miehei lipase and the putative active site residues were strictly conserved.
Central Research Institute, Fuji Oil Co., Ltd., Ibaraki, Japan.
W
W
Kugimiya
Y
Y
Otani
M
M
Kohno
Y
Y
Hashimoto
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004271:DNA, Fungal / Q000235:genetics*; D005656:Fungal Proteins / Q000235:genetics*; D015870:Gene Expression / Q000235:genetics; D008049:Lipase / Q000235:genetics*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009090:Mucorales / Q000201:enzymology / Q000235:genetics; D012233:Rhizopus / Q000201:enzymology* / Q000235:genetics
D004271:DNA, Fungal; D005656:Fungal Proteins; D008049:Lipase