Enzyme production by recombinant Trichoderma reesei strains.
The production of both homologous and heterologous proteins with the cellulolytic filamentous fungus Trichoderma reesei is described. Biotechnically important improvements in the production of cellulolytic enzymes have been obtained by genetic engineering methodology to construct strains secreting novel mixtures of cellulases. These improvements have been achieved by gene inactivation and promoter changes. The strong and highly inducible promoter of the gene encoding the major cellulase, cellobiohydrolase I (CBHI) has also been used for the production of eukaryotic heterologous proteins in Trichoderma. The expression and secretion of active calf chymosin is described in detail.
VTT, Biotechnical Laboratory, Espoo, Finland.
J M
JM
Uusitalo
K M
KM
Nevalainen
A M
AM
Harkki
J K
JK
Knowles
M E
ME
Penttilä
D000818:Animals; D002417:Cattle; D043366:Cellulose 1,4-beta-Cellobiosidase; D002918:Chymotrypsin / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D003470:Culture Media / Q000494:pharmacology; D004790:Enzyme Induction / Q000187:drug effects; D005800:Genes, Fungal; D005822:Genetic Vectors; D006026:Glycoside Hydrolases / Q000096:biosynthesis / Q000235:genetics*; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis* / Q000378:metabolism; D014242:Trichoderma / Q000235:genetics*
D003470:Culture Media; D011993:Recombinant Fusion Proteins; D006026:Glycoside Hydrolases; D043366:Cellulose 1,4-beta-Cellobiosidase; D002918:Chymotrypsin