Adrenergic regulation of neonatal brown fat adenylyl cyclase and Gs alpha activity.
Norepinephrine (NE)-stimulated adenylyl cyclase (AC) activity increases during the perinatal period in rat brown adipose tissue (BAT), and this increase is associated with changes in the activities of both the catalytic subunit (C) and Gs alpha, the GTP-binding protein that mediates activation of C. The present study examined the role of the sympathetic nervous system in the postnatal sensitization of AC. The sympathetic innervation of BAT increased 7- to 13-fold after birth, and this increase was temporally correlated with the postnatal enhancement of AC responsiveness. 6-hydroxydopamine (6-HDA) treatment of neonates reduced tyrosine hydroxylase levels by greater than 90%. This treatment greatly reduced the perinatal increase in NE- and NaF-stimulated AC and completely abolished the increase in forskolin-Mn(2+)-stimulated activity. However, sympathectomy did not alter the postnatal increase in Gs alpha-specific activity and did not prevent the postnatal reduction in Gs alpha levels. These results demonstrate that the sympathetic innervation of BAT develops fully after birth and is essential for the postnatal increase in the activity of C but not of Gs alpha.
Department of Psychiatry, Wayne State University School of Medicine, Detroit, Michigan 48235.
A
A
Chaudhry
J G
JG
Granneman
D000262:Adenylyl Cyclases / Q000737:chemistry / Q000378:metabolism*; D002001:Adipose Tissue, Brown / Q000196:embryology / Q000294:innervation / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism / Q000502:physiology*; D002384:Catalysis; D005333:Fetus / Q000378:metabolism; D019204:GTP-Binding Proteins / Q000378:metabolism*; D016627:Oxidopamine / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D013564:Sympathetic Nervous System / Q000196:embryology / Q000254:growth & development / Q000502:physiology*; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
D016627:Oxidopamine; D014446:Tyrosine 3-Monooxygenase; D019204:GTP-Binding Proteins; D000262:Adenylyl Cyclases