Primary structures of N-linked oligosaccharides of momordin-a, a ribosome-inactivating protein from Momordica charantia seeds.
The structures of the N-linked oligosaccharides of momordin-a, which is a ribosome-inactivating protein from the seeds of Momordica charantia, were analyzed. First, the N-linked oligosaccharides of this glycoprotein were liberated by hydrazinolysis. After N-acetylation, the reducing ends of the oligosaccharides were coupled with 2-aminopyridine and the pyridylamino (PA-) derivatives were purified by gel filtration and high performance liquid chromatography (HPLC) on an ODS-silica column. Three kinds of oligosaccharide fractions were separated by HPLC. The structure of each oligosaccharide isolated was analyzed by a combination of sugar component analysis, exoglycosidase digestion, another kind of HPLC using an amide-silica column, and 500-MHz 1H NMR spectroscopy. The structures of two main oligosaccharides were established to be: [Formula; see text] and [Formula; see text]. These two oligosaccharides were the first examples having xylose (or fucose) but no alpha-mannosyl linkage among the N-linked oligosaccharides of glycoproteins from both animal and plant origins.
Department of Agricultural Science, Faculty of Agriculture, Okayama University, Japan.
Y
Y
Kimura
Y
Y
Minami
T
T
Tokuda
S
S
Nakajima
S
S
Takagi
G
G
Funatsu
D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009699:N-Glycosyl Hydrolases; D009844:Oligosaccharides / Q000737:chemistry* / Q000302:isolation & purification; D010940:Plant Proteins / Q000737:chemistry*; D054790:Ribosome Inactivating Proteins, Type 2; D012639:Seeds / Q000737:chemistry; D014994:Xylose / Q000737:chemistry
D009844:Oligosaccharides; D010940:Plant Proteins; D054790:Ribosome Inactivating Proteins, Type 2; D014994:Xylose; D009699:N-Glycosyl Hydrolases