Fermentation and isolation of herbicolin A, a peptide antibiotic produced by Erwinia herbicola strain A 111.
Erwinia herbicola (Enterobacter agglomerans), belonging to the Enterobacteriaceae, produces the lipopeptide antibiotics herbicolin A and B, which are active against sterol-containing fungi. Fermentation of these antibiotics was performed in 20-l stirred glass fermentors in a batch process. Best yields of antibiotic production were found at low cultivation temperatures in a TRIS-buffered chemically defined medium. Under these conditions the amount of impurities aggravating the purification was minimized. Isolation was performed by adsorption, and gel and ion exchange chromatographic techniques. In a final purification step preparative high performance liquid chromatography (PHPLC) yielded pure herbicolin A.
Greiner
M
M
Institut für Biologie I, Mikrobiologie I, Universität Tübingen, Federal Republic of Germany.
Winkelmann
G
G
D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents; D000935:Antifungal Agents / Q000378:metabolism*; D023181:Antimicrobial Cationic Peptides; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D004885:Erwinia / Q000378:metabolism*; D005285:Fermentation; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000378:metabolism; D010455:Peptides; D013696:Temperature
D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D023181:Antimicrobial Cationic Peptides; D009842:Oligopeptides; D010455:Peptides; C027099:herbicolin A; C064202:herbicolin B