Chronic intrajejunal TNBS application in TNBS-sensitized rats: a new model of chronic inflammatory bowel diseases.
An enteritis, based on a delayed-type hypersensitivity reaction, was induced in TNBS (2,4,4-trinitrobenzenesulfonic acid) sensitized rats by intrajejunal challenge with TNBS. This treatment induced a chronic inflammation of the distal small intestine, characterized by gross hyperaemia and oedema, as assessed by a macroscopic score. Histologically, the inflammatory response included cell infiltration by lymphocytes and histiocytes, a transmural granulomatous inflammation with multinucleated cells and activated mesenteric lymph nodes. Drug treatment with sulfasalazine or 5-aminosalicylic acid improved enteritis score. The applicability and relevance of this new model is discussed in relation to drug development and basic research of inflammatory bowel diseases.
Department of Pharmacology, Center of Research, Grünenthal GmbH, Aachen, FRG.
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D000636:Aminosalicylic Acids / Q000008:administration & dosage / Q000494:pharmacology; D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D007114:Immunization; D015212:Inflammatory Bowel Diseases / Q000139:chemically induced / Q000188:drug therapy / Q000473:pathology; D007413:Intestinal Mucosa / Q000187:drug effects* / Q000473:pathology; D007583:Jejunum; D019804:Mesalamine; D051381:Rats; D017207:Rats, Sprague-Dawley; D012460:Sulfasalazine / Q000008:administration & dosage / Q000494:pharmacology; D014302:Trinitrobenzenesulfonic Acid / Q000008:administration & dosage / Q000276:immunology / Q000633:toxicity*
D000636:Aminosalicylic Acids; D012460:Sulfasalazine; D019804:Mesalamine; D014302:Trinitrobenzenesulfonic Acid