Atomic structure of single-stranded DNA bacteriophage phi X174 and its functional implications.
The mechanism of DNA ejection, viral assembly and evolution are related to the structure of bacteriophage phi X174. The F protein forms a T = 1 capsid whose major folding motif is the eight-stranded antiparallel beta barrel found in many other icosahedral viruses. Groups of 5 G proteins form 12 dominating spikes that enclose a hydrophilic channel containing some diffuse electron density. Each G protein is a tight beta barrel with its strands running radially outwards and with a topology similar to that of the F protein. The 12 'pilot' H proteins per virion may be partially located in the putative ion channel. The small, basic J protein is associated with the DNA and is situated in an interior cleft of the F protein. Tentatively, there are three regions of partially ordered DNA structure,
McKenna
R
R
Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907.
Xia
D
D
Willingmann
P
P
Ilag
L L
LL
Krishnaswamy
S
S
Rossmann
M G
MG
Olson
N H
NH
Baker
T S
TS
Incardona
N L
NL
D000595:Amino Acid Sequence; D010584:Bacteriophage phi X 174 / Q000737:chemistry / Q000235:genetics / Q000648:ultrastructure*; D002213:Capsid / Q000737:chemistry; D004279:DNA, Viral / Q000737:chemistry / Q000378:metabolism; D007473:Ion Channels / Q000737:chemistry / Q000378:metabolism; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009154:Mutation; D011487:Protein Conformation; D014764:Viral Proteins / Q000737:chemistry; D015678:Viral Structural Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D014961:X-Ray Diffraction
D004279:DNA, Viral; D007473:Ion Channels; D014764:Viral Proteins; D015678:Viral Structural Proteins