A new method for estimating population doubling time of vertebrate cells.
Based upon the fact that the amount of DNA doubles after each cycle, a technique was developed in which the population doubling time for cell cultures was determined from the ratio of its DNA content to [3H]thymidine incorporation under conditions in which cell numbers cannot be readily determined. This technique may be useful in determining what proportion the sample is of the total cell population.
Tripathi
S C
SC
Center for Environmental Health Sciences, Massachusetts Institute of Technology, Cambridge.
Donaghey
C E
CE
D000818:Animals; D002453:Cell Cycle; D002478:Cells, Cultured; D002645:Chickens; D004247:DNA / Q000378:metabolism*; D005347:Fibroblasts / Q000378:metabolism; D009206:Myocardium / Q000166:cytology; D013936:Thymidine / Q000378:metabolism
D004247:DNA; D013936:Thymidine