Genetic transformation of Aureobasidium pullulans.
Aureobasidium pullulans strain Y117 was transformed to hygromycin resistance using plasmid pDH33, which contains the bacterial hygromycin B phosphotransferase gene (hph) fused to promoter elements of the Aspergillus niger glucoamylase gene (glaA). Southern hybridizations of transformants revealed multiple, integrated copies of the vector. The glaA promoter was not induced by starch in A. pullulans as it is in A. niger; however, the transcriptional start points were the same in both species.
Institute for Microbial and Biochemical Technology, Forest Products Laboratory, U.S. Department of Agriculture, Madison, Wisconsin 53705-2398.
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D001234:Aspergillus niger / Q000235:genetics; D015139:Blotting, Southern; D002934:Cinnamates; D004352:Drug Resistance, Microbial / Q000235:genetics; D005087:Glucan 1,4-alpha-Glucosidase / Q000235:genetics; D006921:Hygromycin B / Q000031:analogs & derivatives / Q000494:pharmacology; D003904:Mitosporic Fungi / Q000187:drug effects / Q000235:genetics*; D010770:Phosphotransferases / Q000235:genetics; D017853:Phosphotransferases (Alcohol Group Acceptor); D010957:Plasmids; D014170:Transformation, Genetic
D002934:Cinnamates; D006921:Hygromycin B; C026273:hygromycin A; D010770:Phosphotransferases; D017853:Phosphotransferases (Alcohol Group Acceptor); C063242:hygromycin-B kinase; D005087:Glucan 1,4-alpha-Glucosidase