Prognostic B-cell epitopes on the flagellar protein of Borrelia burgdorferi.
Overlapping decapeptides based on the flagellin sequence of Borrelia burgdorferi B31 (G. S. Gassmann, M. Kramer, U. B. Göbel, and R. Wallich, Nucleic Acids Res. 17:3590, 1989) were used to identify immunologically reactive regions of flagellin. Five serum specimens from patients with late manifestations of Lyme disease and borrelia-specific monoclonal antibody H9724 reacted with an epitope in the central region of the flagellar protein (amino acids 205 to 226), which is heterologous to the amino acid sequences of other bacterial flagellins. This epitope was not recognized by human sera with antibodies to Treponema pallidum, sera of healthy individuals, or sera from patients with stage I or II of Lyme disease.
Freie Universität Berlin, Rudolf Virchow Krankenhaus, Neurologie, Germany.
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Kölmel
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology*; D015748:Borrelia burgdorferi Group / Q000276:immunology*; D003429:Cross Reactions; D000939:Epitopes / Q000276:immunology*; D005260:Female; D005408:Flagellin / Q000235:genetics / Q000276:immunology*; D006801:Humans; D008193:Lyme Disease / Q000276:immunology; D008297:Male; D008969:Molecular Sequence Data
D000911:Antibodies, Monoclonal; D000939:Epitopes; D005408:Flagellin