Acceptor specificity of cyclodextrin glycosyltransferase from Bacillus stearothermophilus and synthesis of alpha-D-glucosyl O-beta-D-galactosyl-(1----4)-beta-D-glucoside.
Bacillus stearothermophilus CGTase had a wider acceptor specificity than Bacillus macerans CGTase did and produced large amounts of transfer products of various acceptors such as D-galactose, D-mannose, D-fructose, D- and L-arabinose, D- and L-fucose, L-rhamnose, D-glucosamine, and lactose, which were inefficient acceptors for B. macerans CGTase. The main component of the smallest transfer products of lactose was assumed to be alpha-D-glucosyl O-beta-D-galactosyl-(1----4)-beta-D-glucoside.
Kitahata
S
S
Osaka Municipal Technical Research Institute, Japan.
Hara
K
K
Fujita
K
K
Nakano
H
H
Kuwahara
N
N
Koizumi
K
K
D001407:Bacillus / Q000201:enzymology; D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid; D001411:Geobacillus stearothermophilus / Q000201:enzymology*; D005964:Glucosyltransferases / Q000378:metabolism*; D007785:Lactose / Q000378:metabolism; D008969:Molecular Sequence Data; D009005:Monosaccharides / Q000378:metabolism; D013213:Starch / Q000378:metabolism; D013379:Substrate Specificity; D014312:Trisaccharides / Q000378:metabolism*
D009005:Monosaccharides; D014312:Trisaccharides; C078115:glucosyl-O-galactosyl-(1-4)glucoside; D013213:Starch; D005964:Glucosyltransferases; C019963:cyclomaltodextrin glucanotransferase; D007785:Lactose