Simple system to irradiate cultured cells with UVA light.
We developed a simple system for the irradiation of cultured cells with ultraviolet-A (UVA) light, and used it to study the colony-forming ability of fibroblasts in culture after UVA treatment. The system requires an ordinary clean bench, and the other materials and devices needed, such as dishes with a 35-mm diameter, plate glass, and 15-W UVA lamps, are easy to obtain and assemble. Neither stirring nor cooling of cells is required, because the fan of the clean bench acts as a cooler, and cells are irradiated from the bottom after they have settled in the dish. The UVA light incident on cells after its passage through both the plate glass and the bottom part of the dish contains so little of UVB wavelengths that cell killing by pyrimidine dimers was negligible.
Sato
K
K
Department of Dermatology, Osaka University School of Medicine, Japan.
Yoshikawa
K
K
D002470:Cell Survival / Q000528:radiation effects; D002478:Cells, Cultured / Q000528:radiation effects*; D003584:Cytological Techniques / Q000295:instrumentation; D006801:Humans; D008722:Methods; D012867:Skin / Q000166:cytology / Q000528:radiation effects; D014466:Ultraviolet Rays