A method for the preparation of coimmobilizates by adhesion using polyethylenimine.
A method has been described for obtaining coimmobilizates by the simultaneous binding of glucose oxidase to the cell and the enzyme-bound cell to cotton thread through adhesion using polyethylenimine (PEI). Glucose oxidase was found to adsorb onto PEI-coated yeast cells from a water suspension. The desorption observed at higher ionic strength could be obviated by cross-linking with 2% glutaraldehyde for 2 min. The enzyme-bound yeast cells could then be immobilized by adhesion on cotton thread. The coimmobilizate could be reused for over 10 batches without appreciable loss in activity.
Food Technology and Enzyme Engineering Division, Bhabha Atomic Research Centre, Bombay, India.
S F
SF
D'Souza
J S
JS
Melo
D004800:Enzymes, Immobilized / Q000378:metabolism; D005949:Glucose Oxidase / Q000378:metabolism*; D007700:Kinetics; D011094:Polyethyleneimine; D011485:Protein Binding; D012441:Saccharomyces cerevisiae / Q000378:metabolism*
D004800:Enzymes, Immobilized; D011094:Polyethyleneimine; D005949:Glucose Oxidase