Purification of testicular transglutaminase by hydrophobic chromatography on phenyl-sepharose.
We developed a procedure for purification to homogeneity of cytosolic transglutaminase from bovine testes, by means of anion exchange, size exclusion and hydrophobic chromatography. The crucial step is chromatography on Phenyl sepharose, with specific elution by the inhibitor GTP. The enzyme displays the properties of type 2 transglutaminases.
Istituto di Chimica Biologica, Università di Ferrara, Italy.
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D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002417:Cattle; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D006160:Guanosine Triphosphate / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008297:Male; D008970:Molecular Weight; D012685:Sepharose / Q000031:analogs & derivatives; D013696:Temperature; D013737:Testis / Q000201:enzymology*; D011503:Transglutaminases / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism
C020927:phenyl-sepharose; D006160:Guanosine Triphosphate; D012685:Sepharose; D011503:Transglutaminases; D002118:Calcium