Uptake of thyroxine by cultured human adipocyte precursors isolated from lean and obese subjects.
The mechanism of thyroxine uptake by human adipocyte precursors has been studied in primary culture. Also the rates of transport of this hormone into the isolated cells of adipose tissue were compared for lean and obese subjects. It was demonstrated that thyroxine transport into the human adipocyte precursor cells is an active, energy-dependent process characterized by very low rate (Km = 10 pmol/l, Vmax = 8 fmol FT4/10(6) cells/min.). By comparing the rates of thyroxine transport into the precursor cells of adipocytes isolated from adipose tissue of lean and obese subjects it was possible to demonstrate a clear tendency to the lowered rate of transport of thyroxine to the cells in obesity. The results of this study suggest that the lowered rate of thyroxine transport to preadipocytes and adipocytes observed in obesity may significantly influence the metabolic state of these cells.
Department of Biochemistry, Medical Center of Postgraduate Education, Warsaw, Poland.
G
G
Sypniewska
J
J
Nauman
W
W
Tarnowski
D017667:Adipocytes / Q000187:drug effects / Q000378:metabolism*; D000328:Adult; D002478:Cells, Cultured; D006801:Humans; D007457:Iodine Radioisotopes; D008875:Middle Aged; D009765:Obesity / Q000378:metabolism*; D010042:Ouabain / Q000494:pharmacology; D011190:Potassium Cyanide / Q000494:pharmacology; D013974:Thyroxine / Q000493:pharmacokinetics*
D007457:Iodine Radioisotopes; D010042:Ouabain; D011190:Potassium Cyanide; D013974:Thyroxine