Streptococcal pyrogenic exotoxin A and streptolysin O enhance polymorphonuclear leukocyte binding to gelatin matrixes.
Autopsy data from cases of streptococcal toxic shock demonstrate accumulation of polymorphonuclear leukocytes (PMNL) within lung and soft tissue microvasculature. Because of the increased prevalence of streptococcal pyrogenic exotoxin A (SPEA)-producing strains associated with streptococcal toxic shock syndrome, experiments were done to determine whether SPEA or streptolysin O (SLO, a thiol-activated cytolysin produced by all group A streptococci) could stimulate PMNL-dependent adherence mechanisms in vitro. SPEA (0.01-10 micrograms/5.5 x 10(6) PMNL) only modestly enhanced PMNL adherence over the entire range of concentrations tested. In contrast, SLO-induced PMNL binding was highly dose dependent (maximal binding, 55.1 +/- 1.6% at 0.5 hemolytic units/5.5 x 10(6) PMNL) and was mediated by CD11/CD18 adherence glycoprotein.
Bryant
A E
AE
VA Medical Center, Infectious Diseases Section, Boise, Idaho 83702.
Kehoe
M A
MA
Stevens
D L
DL
D015703:Antigens, CD / Q000502:physiology; D001426:Bacterial Proteins; D018845:CD11 Antigens; D018821:CD18 Antigens; D002448:Cell Adhesion / Q000187:drug effects; D002478:Cells, Cultured; D005098:Exotoxins / Q000494:pharmacology*; D006801:Humans; D008565:Membrane Proteins; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism*; D011749:Pyrogens / Q000494:pharmacology*; D016028:Receptors, Leukocyte-Adhesion / Q000502:physiology; D013301:Streptolysins / Q000494:pharmacology*
D015703:Antigens, CD; D001426:Bacterial Proteins; D018845:CD11 Antigens; D018821:CD18 Antigens; D005098:Exotoxins; D008565:Membrane Proteins; D011749:Pyrogens; D016028:Receptors, Leukocyte-Adhesion; C117980:SpeA protein, Streptococcus pyogenes; D013301:Streptolysins; C027951:erythrogenic toxin; C025964:streptolysin O