[Polymerase chain reaction (PCR) in microbiology. Value and practical applications].
The gene amplification reaction (PCR) developed in 1985 is a molecular biology instrument which is used to amplify genomic restriction fragment lengths of micro-organisms found in biological samples. At the moment, its applications to direct microbiological diagnosis remain to be determined precisely, and its strict technique is awaiting standardization. However, it is a powerful tool for molecular typing used to follow up an antiviral therapy or to analyse retrospectively paraffin-embedded tissue banks in search of viral or bacterial nucleic acids.
Service de Bactériologie-Virologie, Hôpital Saint-Louis, Paris.
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F
Morinet
D001424:Bacterial Infections / Q000175:diagnosis*; D006800:Deltaretrovirus Infections / Q000175:diagnosis; D006505:Hepatitis / Q000175:diagnosis; D004854:Herpesvirus 4, Human / Q000145:classification; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D014412:Tumor Virus Infections / Q000145:classification; D014777:Virus Diseases / Q000175:diagnosis*
Réaction de polymérisation en chaîne (PCR) en microbiologie. Intérêt et applications pratiques.