Hypercalcaemia and pancreatic ultrastructure in cats.
OBJECTIVE
To study the effects of local and systemic infusions of calcium on the ultrastructure of the pancreas in cats.


METHODS
Controlled study.


METHODS
Three groups of four cats each had local infusions (into the splenic artery) of calcium gluconate 0.6 mmol/kg.hour or potassium chloride 1.1 mmol/kg.hour, or sodium chloride 0.9%, for three hours. Two groups of eight cats each had systemic infusions (into the jugular vein) of either calcium gluconate 0.6 mmol/kg.hour or sodium chloride 0.9%, for twelve hours. In the group that was given calcium, the infusion rate was reduced after three hours to 0.3 mmol/kg.hour to maintain the hypercalcaemic state for a further nine hours.


RESULTS
Local infusion of calcium caused destruction of acinar cells with hydropic degeneration of nuclei, discharge of cell organelles into the interstitial spaces, and extravasation of red blood cells but no apparent damage to the capillaries. There were no ultrastructural changes of any importance in the groups that received potassium or sodium chloride. Systemic infusion of calcium resulted in a 1.8 fold increase in the ionised calcium concentration in the serum, progressive signs of overstimulation of the Golgi apparatus with hypertrophy, fusion of condensing vacuoles, and disruption of the acinar cell polarization. This was followed by clumping of nuclear chromatin and destruction of acinar cells.


CONCLUSIONS
Acute pancreatitis in cats can result from stimulation and destruction of acinar cells by hypercalcaemia.
Department of Surgery, University of Minnesota Hospitals, Minneapolis.
T W
TW
Frick
M A
MA
Spycher
P U
PU
Heitz
F
F
Largiadèr
R L
RL
Goodale
D000208:Acute Disease; D000818:Animals; D002118:Calcium / Q000008:administration & dosage / Q000009:adverse effects*; D002415:Cats; D006934:Hypercalcemia / Q000139:chemically induced / Q000473:pathology; D007261:Infusions, Intra-Arterial; D007262:Infusions, Intravenous; D008854:Microscopy, Electron; D010179:Pancreas / Q000187:drug effects / Q000473:pathology / Q000648:ultrastructure*; D010195:Pancreatitis / Q000139:chemically induced* / Q000473:pathology
D002118:Calcium