Production of recombinant protein C in serum-containing and serum-free perfusion culture.
For the development of a perfusion culture producing recombinant human protein C, the effects of fetal calf serum and growth factors on cell growth and recombinant protein production were investigated. Although the growth of recombinant cells was stimulated by serum in a dose-dependent manner, a lower concentration of serum (2%) could support both synthesis and post-translational modification of protein C as efficiently as 10% serum. Among the growth factors tested, transferrin enhanced protein C production to the level comparable with 10% serum, while insulin was effective in maintaining cellular metabolism. Based on these results, a perfusion culture for a scale-up production of recombinant protein C was done using an Opticell culture system. A good productivity of the recombinant protein was obtained in low serum or serum-free medium for more than one month.
Laboratory for Cell Biology, Pharma Research Laboratories, Hoechst Japan Ltd., Saitama.
T
T
Sugiura
H B
HB
Maruyama
D002455:Cell Division; D002460:Cell Line; D016895:Culture Media, Serum-Free; D004797:Enzyme-Linked Immunosorbent Assay; D007700:Kinetics; D010477:Perfusion; D011486:Protein C / Q000096:biosynthesis* / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis*
D016895:Culture Media, Serum-Free; D011486:Protein C; D011994:Recombinant Proteins