Physiological analysis of motor reorganization following lower limb amputation.
It is now known that amputation results in reorganization of central motor pathways, but the mechanism for the changes is unclear. One possibility is alteration of the excitability of the alpha motoneurons. We studied motor reorganization and excitability of alpha motoneurons to Ia input in 6 subjects with unilateral lower limb amputation. A Cadwell MES-10 stimulator was used to deliver transcranial magnetic stimuli through a circular coil centered on the sagittal axis 4 cm anterior to Cz and through an 8-shaped coil positioned over scalp locations 1 cm apart along the coronal axis. Surface EMG was recorded bilaterally from quadriceps femoris, the first muscle immediately proximal to the site of amputation. Excitability of the spinal alpha motoneuron pool to Ia afferents was assessed by determining the ratio of the maximal H reflex to the maximal M response (H/M ratio) elicited in the quadriceps femoris. Stimuli of equal intensity delivered to optimal scalp positions recruited a larger percentage of the alpha motoneuron pool in muscles ipsilateral to the stump than in those contralateral to the stump (P less than 0.01). Mean onset latencies of motor evoked potentials were shorter in ipsilateral muscles than in contralateral muscles (P less than 0.01). Muscles ipsilateral to the stump showed a trend toward activation from a larger number of scalp positions than those contralateral to the stump (P = 0.06). There was no difference in the quadriceps H/M ratios (7.2% ipsilateral vs. 10.9% contralateral). The absence of changes in the excitability of the alpha motoneuron pool in the presence of motor reorganization targeting muscles proximal to the stump suggests that reorganization occurs proximal to the alpha motoneuron level.
Fuhr
P
P
Human Cortical Physiology Unit, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892.
Cohen
L G
LG
Dang
N
N
Findley
T W
TW
Haghighi
S
S
Oro
J
J
Hallett
M
M
D000328:Adult; D000368:Aged; D000671:Amputation, Surgical; D004576:Electromyography; D005071:Evoked Potentials / Q000502:physiology; D005260:Female; D006181:H-Reflex / Q000502:physiology; D006801:Humans; D007866:Leg / Q000294:innervation / Q000502:physiology / Q000601:surgery; D008280:Magnetics; D008297:Male; D008875:Middle Aged; D009044:Motor Cortex / Q000502:physiology*; D009132:Muscles / Q000502:physiology; D009473:Neuronal Plasticity / Q000502:physiology*; D011930:Reaction Time