Inhibition of the transepithelial potential difference and short circuit current in the isolated frog skin by alloxan.
1. Electrical parameters: short circuit current (SCC), transepithelial potential difference (PD) and electrical resistance (R) were measured in isolated frog skin (Rana pipiens) in the presence and in the absence of alloxan. 2. Alloxan decreased SCC and PD in a concentration-dependent pattern, while R remained unchanged. 3. The effect on SCC and PD was observed after 25 min of exposure to the drug. Maximal average effect was 20% in SCC and 17.5% in PD. 4. These results suggest that alloxan decreased epithelial sodium transport, through interference with the activity of the Na(+)-K(+)-ATPase.
Departamento de Fisiología y Biofísica, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, México, D.F.
C
C
Soto
J L
JL
Reyes
A
A
Ramirez
F
F
Paz
C
C
Perez
D000496:Alloxan / Q000494:pharmacology*; D000818:Animals; D001681:Biological Assay; D004553:Electric Conductivity / Q000187:drug effects; D004848:Epithelium / Q000187:drug effects; D008564:Membrane Potentials / Q000187:drug effects; D011894:Rana pipiens; D051381:Rats; D012867:Skin / Q000187:drug effects*; D000254:Sodium-Potassium-Exchanging ATPase / Q000187:drug effects*
D000496:Alloxan; D000254:Sodium-Potassium-Exchanging ATPase