A model for the prediction of partition coefficients in aqueous two-phase systems.
A mathematical model has been developed to predict partition coefficients for aqueous two-phase systems. The model is based on a previously-developed equation for partitioning which arises from an osmotic pressure viral expansion. The model suggests that the properties of importance are the concentration difference of one of the phase-forming components, such as a polymer, and the hydrophobicity of the solute relative to the hydrophobic-hydrophilic difference between the two phases. Several two-phase systems have been studied, with a particular emphasis on the poly(ethylene glycol)/magnesium sulfate system. Numerous solutes, including peptides, were used in this system and their partition coefficients show good agreement with the model.
Center for Bioprocess and Product Development, Department of Chemical Engineering, University of Virginia, Charlottesville 22903-2442.
M A
MA
Eiteman
J L
JL
Gainer
D008433:Mathematics; D008962:Models, Theoretical; D010455:Peptides / Q000302:isolation & purification; D011092:Polyethylene Glycols; D012995:Solubility
D010455:Peptides; D011092:Polyethylene Glycols