Chromatographic evidence for the presence of multiple tachykinins in the bovine adrenal medulla.
Among the mammalian tachykinins, substance P (SP) has been shown to be the most potent at modulating the response due to nicotinic acetylcholine receptor stimulation of bovine adrenal chromaffin cells. SP-like immunoreactivity has been detected in nerve terminals innervating the adrenal medulla; however, little is known of the presence of other tachykinins in this tissue. In this study, reverse-phase HPLC was used to fractionate peptides in bovine adrenal medullary extracts, and the fractions were analyzed by radioimmunoassay using antisera to SP or neurokinin A (NKA). The results show that both NKA- and SP-like immunoreactivities are present in the adrenal medulla. The presence of neurokinin B is also indicated. The presence of multiple tachykinins in this tissue raises questions as to their functions in the adrenal medulla.
Basile
S
S
Russell Grimwade School of Biochemistry, University of Melbourne, Parkville, Victoria, Australia.
Cheung
N S
NS
Livett
B G
BG
D000313:Adrenal Medulla / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D015288:Neurokinin A / Q000378:metabolism; D009479:Neuropeptides / Q000378:metabolism; D010446:Peptide Fragments / Q000378:metabolism; D011863:Radioimmunoassay; D013373:Substance P / Q000378:metabolism; D015320:Tachykinins / Q000737:chemistry / Q000378:metabolism*
D009479:Neuropeptides; D010446:Peptide Fragments; D015320:Tachykinins; C059905:tachykinin neuropeptide gamma; D013373:Substance P; D015288:Neurokinin A