Induction of MHC class I antigen expression following infection of a human esthesioneuroblastoma cell line with cytomegalovirus and human immunodeficiency virus.
Productive infections with cytomegalovirus (CMV) and human immunodeficiency virus (HIV) were established in the Tp41ON cell line derived from a human esthesioneuroblastoma. HIV antigen expression was highest in cultures coinfected with CMV and HIV. Viral infection caused increased MHC class I antigen expression while class II and CD4 antigens remained undetectable using immunofluorescence methods. Uninfected cultures showed 10% and coinfected cultures 80% class I antigen positive cells. In coinfected cultures, CMV and HIV antigens were detected in 4% and 8% of the cells, respectively. The detection of CMV antigens in some multinucleated cells suggests coinfection with both viruses in these cells, as multinucleated cells were not found in cultures infected with CMV only. The study shows that a cell line showing neuronal differentiation in vitro can be infected with CMV and HIV and that this infection increases MHC class I antigen expression.
Department of Neurosurgery, Karolinska Institute, Stockholm, Sweden.
T
T
Mathiesen
V P
VP
Collins
E
E
Ohlsson
V A
VA
Sundqvist
B
B
Wahren
D000951:Antigens, Neoplasm / Q000096:biosynthesis*; D000956:Antigens, Viral / Q000096:biosynthesis; D015704:CD4 Antigens / Q000032:analysis; D002454:Cell Differentiation; D003587:Cytomegalovirus / Q000502:physiology*; D003588:Cytopathogenic Effect, Viral; D015972:Gene Expression Regulation, Neoplastic; D015488:HIV Antigens / Q000096:biosynthesis; D015497:HIV-1 / Q000502:physiology*; D006680:HLA Antigens / Q000096:biosynthesis*; D006681:HLA-D Antigens / Q000032:analysis; D006801:Humans; D018241:Neuroectodermal Tumors, Primitive, Peripheral / Q000276:immunology* / Q000473:pathology; D014407:Tumor Cells, Cultured / Q000276:immunology / Q000382:microbiology
D000951:Antigens, Neoplasm; D000956:Antigens, Viral; D015704:CD4 Antigens; D015488:HIV Antigens; D006680:HLA Antigens; D006681:HLA-D Antigens