The micronucleus test of benzo[a]pyrene with mouse and rat peripheral blood reticulocytes.
The micronucleus test using peripheral blood reticulocytes (RETs) was evaluated in CD-1 and BDF1 mice and Sprague-Dawley rats treated with benzo[a]pyrene at two independent laboratories. The maximum incidence of micronucleated reticulocytes (MNRETs) appeared in both strains of mice 48 h after the treatment; interlaboratory differences were small. The incidence of MNRETs in BDF1 mice was higher than in CD-1 mice. In rats, significant increases of MNRETs with the maximum response at 72 h were detected when B[a]P was administered i.p.; slight but significant increases were observed at 24 h or later, with the maximum at 24-48 h, when it was administered p.o. These results suggest that the new method for the micronucleus test using circulating RETs will be useful in the detection of the clastogenicity of chemicals.
Developmental Research Laboratory of Daiiauchi Pharmaceutical Co., Ltd., Tokyo, Japan.
H
H
Shimada
H
H
Suzuki
S
S
Itoh
C
C
Hattori
Y
Y
Matsuura
S
S
Tada
C
C
Watanabe
D000818:Animals; D001564:Benzo(a)pyrene / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D015162:Micronucleus Tests / Q000379:methods; D016685:Mitomycin / Q000633:toxicity; D009153:Mutagens / Q000633:toxicity; D051381:Rats; D011919:Rats, Inbred Strains; D012156:Reticulocytes / Q000187:drug effects*
D009153:Mutagens; D001564:Benzo(a)pyrene; D016685:Mitomycin