The effect of pH on the production of pertussis toxin by Bordetella pertussis.
The production of pertussis toxin by Bordetella pertussis was increased by controlling the pH at 7.0 through the addition of sulfuric acid. The more commonly used hydrochloric acid and Tris buffer were observed to be detrimental to toxin yields.
Licari
P
P
Biotechnology Engineering Center, Tufts University, Medford, MA 02155.
Winberry
L
L
Swartz
R
R
D001886:Bordetella pertussis / Q000187:drug effects* / Q000378:metabolism; D003470:Culture Media / Q000494:pharmacology; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D006851:Hydrochloric Acid / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D037342:Pertussis Toxin; D013464:Sulfuric Acids / Q000494:pharmacology; D014325:Tromethamine / Q000494:pharmacology; D010566:Virulence Factors, Bordetella / Q000096:biosynthesis*
D003470:Culture Media; D013464:Sulfuric Acids; D010566:Virulence Factors, Bordetella; D014325:Tromethamine; D037342:Pertussis Toxin; C033158:sulfuric acid; D006851:Hydrochloric Acid