Simultaneous diagnosis of coagulation factor XIIA (F13A) and plasminogen (PLG) phenotypes by polyacrylamide gel isoelectric focusing.
In this paper, we report a simple rapid method for simultaneous determination of Coagulation Factor XIIIA (F13A) and plasminogen (PLG) phenotypes by PAGIF with a nominal pH range of 3.5 to 10, followed by immunofixation and silver stain. Critical considerations concerning the conditions of molecular separation and detection strategies are also presented.
Departamento de Antropoloxía, Facultade de Bioloxía, Universidade de Santiago de Compostela, Galicia, Spain.
J L
JL
Caeiro
C
C
Llano
E
E
Parra
D005819:Genetic Markers; D006579:Heterozygote; D006720:Homozygote; D006801:Humans; D007525:Isoelectric Focusing; D010641:Phenotype; D010958:Plasminogen / Q000032:analysis* / Q000235:genetics; D015203:Reproducibility of Results; D011503:Transglutaminases / Q000032:analysis* / Q000235:genetics
D005819:Genetic Markers; D010958:Plasminogen; D011503:Transglutaminases