Identification and analysis of a genomic strain cluster of mycoplasmalike organisms associated with Canadian peach (eastern) X disease, western X disease, and clover yellow edge.
Genetic interrelatedness among 13 strains of mycoplasmalike organisms (MLOs) from various sources was evaluated by dot hybridization and restriction fragment length polymorphism analyses using cloned DNA probes derived from Canadian peach X (CX) and western X (WX) MLOs. Dot hybridization analysis indicated that CX, WX, and clover yellow edge MLOs are closely related and form a distinct strain cluster that is only distantly related to the 10 other MLOs. Similarity coefficients derived from restriction fragment length polymorphism analysis revealed that CX, WX, and clover yellow edge MLOs represent three distinct genomic types.
Lee
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Microbiology and Plant Pathology Laboratory, USDA Agricultural Research Service, Beltsville, Maryland 20705.
Gundersen
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Davis
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Chiykowski
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D015139:Blotting, Southern; D003001:Cloning, Molecular; D015342:DNA Probes / Q000235:genetics; D009174:Mycoplasma / Q000145:classification* / Q000235:genetics; D010935:Plant Diseases / Q000382:microbiology*; D012150:Polymorphism, Restriction Fragment Length
D015342:DNA Probes