Tumor induction by the LTR, v-src, LTR DNA in four B (MHC) congenic lines of chickens.
We report that the cloned DNA harboring the long terminal repeat (LTR), v-src, LTR proviral structure is tumorigenic in chickens of the Prague congenic lines. The growth rate of these tumors is by far the highest in the recombinant CC.R1 line, the B haplotype of which is composed of the B-F/L4 and B-G12 subregions originating from different naturally occurring haplotypes. Some of the tumors induced by the LTR, v-src, LTR DNA are repeatedly transplantable in syngeneic chickens, maintain unaltered provirus, and express v-src mRNA. Differences in the response to challenge with Rous sarcoma virus (RSV) and LTR, v-src, LTR DNA on a given experimental model are compared and possible involvement of an interaction between B-F/L and B-G region genes is considered. Regression of the LTR, v-src, LTR DNA-induced tumors did not prevent the formation and growth of tumors induced subsequently by RSV.
Department of Cellular and Viral Genetics, Czechoslovak Academy of Sciences, Prague.
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Svoboda
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Plachý
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Hejnar
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Karakoz
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RV
Guntaka
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Geryk
D000818:Animals; D001358:Avian Sarcoma Viruses; D015152:Blotting, Northern; D015139:Blotting, Southern; D002455:Cell Division / Q000235:genetics; D002645:Chickens; D003001:Cloning, Molecular; D004273:DNA, Neoplasm / Q000502:physiology*; D016391:Genes, src / Q000502:physiology*; D005816:Genetic Complementation Test; D009369:Neoplasms / Q000235:genetics* / Q000382:microbiology; D012150:Polymorphism, Restriction Fragment Length; D011533:Proviruses; D012091:Repetitive Sequences, Nucleic Acid / Q000502:physiology*
D004273:DNA, Neoplasm