Lysosomal enzymes produced by immobilized Tetrahymena thermophila.
The ciliated protozoon Tetrahymena thermophila was immobilized for production of secreted lysosomal enzymes in two ways. Cells entrapped in solid Ca-alginate spheres survived but were unable to grow and multiply. However, when encapsulated in hollow Ca-alginate spheres Tetrahymena multiplied well, reaching 0.9 x 10(7) cells/ml. These immobilized cells secreted large amounts of lysosomal enzymes when the medium was changed daily. This system was transferred to a reactor scale using a conical bubble column reactor for semicontinuous cultivation of the encapsulated cells. Under these conditions alpha-glucosidase, beta-glucosidase, beta-hexosaminidase and acid phosphatase were produced for at least 4 weeks. The hollow spheres were stable for 3 months and contained living and secreting Tetrahymena cells during this time. Immobilized T. thermophila cells can thus serve as a good source for production of commercially interesting enzymes.
Zoologisches Institut, Universität Münster, Federal Republic of Germany.
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D000135:Acid Phosphatase / Q000096:biosynthesis*; D000464:Alginates / Q000494:pharmacology; D000818:Animals; D005285:Fermentation; D005959:Glucosidases / Q000096:biosynthesis*; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D008247:Lysosomes / Q000201:enzymology*; D008855:Microscopy, Electron, Scanning; D013768:Tetrahymena / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism* / Q000648:ultrastructure; D001619:beta-N-Acetylhexosaminidases / Q000096:biosynthesis*
D000464:Alginates; D006603:Hexuronic Acids; D020723:Glucuronic Acid; D000135:Acid Phosphatase; D005959:Glucosidases; D001619:beta-N-Acetylhexosaminidases