Nucleotide sequence and PCR-amplification of a polymorphic MboI site in human DNA marker D4S95 linked to the Huntington disease locus.
The nucleotide sequence of the probe BS674E-D defining the genetic locus D4S95 was assessed, and used for designing PCR-primers amplifying a 175 bp fragment containing a polymorphic MboI site. With the described conditions it is now possible to use PCR followed by digestion for rapid determination of genotype in this marker system.
Nørremølle
A
A
Institute of Medical Genetics, University of Copenhagen, Denmark.
Sørensen
S A
SA
Arctander
P
P
D001483:Base Sequence; D015342:DNA Probes; D015252:Deoxyribonucleases, Type II Site-Specific; D005838:Genotype; D006801:Humans; D006816:Huntington Disease / Q000235:genetics*; D008969:Molecular Sequence Data; D010375:Pedigree; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D017422:Sequence Analysis, DNA / Q000379:methods*
D015342:DNA Probes; D015252:Deoxyribonucleases, Type II Site-Specific; C024405:GATC-specific type II deoxyribonucleases