[Riboflavin Reference Standard (Control 891) of National Institute of Hygienic Sciences].
The raw material of riboflavin was examined for the preparation of the "Riboflavin Reference Standard (Control 891)". Analytical data obtained were as follows: melting point, 238.2 degrees C (decomposition); absorbance, E1%1cm = 859 (267 nm), 277 (372 nm), 327 (445 nm); infrared spectrum, same as Riboflavin Reference Standard (Control 872); thin-layer chromatography, 5 impurities were detected; high-performance liquid chromatography, 11 impurities were detected; loss on drying, 0.25%; optical rotation, [alpha]20D = -133.6 degrees; solubility, less than 16 min; assay, 100.1% against Riboflavin Reference Standard (Control 872). Based on the above results, this raw material was authorized to be the Reference Standard of the National Institute of Hygienic Sciences.
Murai
M
M
Kakehi
H
H
Komatsu
H
H
Ishimitsu
S
S
Okada
S
S
D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D006077:Government Agencies; D006920:Hygiene; D007564:Japan; D010602:Pharmacopoeias as Topic; D012256:Riboflavin / Q000302:isolation & purification / Q000592:standards*; D013055:Spectrophotometry, Infrared
D012256:Riboflavin