Sustained enhancement of NMDA receptor-mediated synaptic potential by isoproterenol in rat amygdalar slices.
The effect of isoproterenol (Iso) on synaptic transmission mediated by the N-methyl-D-aspartate (NMDA) receptors (EPSPNMDA) was investigated in slices of rat amygdala using intracellular recording techniques. EPSPNMDA was isolated pharmacologically by application of a solution containing the non-NMDA receptor antagonist 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX, 10 microM) and GABAA receptor blocker bicuculline (20 microM). Superfusion of Iso (15 microM) produced a long-lasting enhancement of EPSPNMDA. Pretreatment the slices with propranolol (10 microM) completely prevented the effect of Iso confirming the mediation by beta-adrenergic receptors. These results provide the direct evidence for adrenergic modulation of excitatory amino acid neurotransmission in the vertebrate central nervous system.
Department of Pharmacology, College of Medicine, National Cheng-Kung University, Tainan City, Taiwan.
P W
PW
Gean
C C
CC
Huang
J H
JH
Lin
J J
JJ
Tsai
D018750:6-Cyano-7-nitroquinoxaline-2,3-dione; D000679:Amygdala / Q000187:drug effects*; D000818:Animals; D001640:Bicuculline / Q000494:pharmacology; D005071:Evoked Potentials / Q000187:drug effects; D066298:In Vitro Techniques; D007545:Isoproterenol / Q000494:pharmacology*; D008564:Membrane Potentials / Q000187:drug effects; D011433:Propranolol / Q000494:pharmacology; D011810:Quinoxalines / Q000494:pharmacology; D051381:Rats; D016194:Receptors, N-Methyl-D-Aspartate / Q000187:drug effects*; D013569:Synapses / Q000187:drug effects*; D013997:Time Factors
D011810:Quinoxalines; D016194:Receptors, N-Methyl-D-Aspartate; D018750:6-Cyano-7-nitroquinoxaline-2,3-dione; D011433:Propranolol; D007545:Isoproterenol; D001640:Bicuculline