Antibody against the Leu-CAM beta-chain (CD18) promotes both LFA-1- and CR3-dependent adhesion events.
The Leukocytic cell-adhesion molecule (beta 2 integrin) family of adhesion molecules play a key role in the intercellular adhesive interactions necessary for normal immune cell function. In this study, we report an antibody that recognizes an epitope on the Leukocytic cell-adhesion molecule common beta-chain (CD18) and promotes both lymphocyte function-associated Ag-1- and CR3-dependent adhesion events. The antibody recognizes a temperature-sensitive epitope that is not dependent on the presence of divalent cations. It is proposed that antibody binding promotes a conformational change in both lymphocyte function-associated Ag-1 and CR3, which may mimic a natural activation mechanism, resulting in increased cellular adhesion.
Celltech Ltd., Slough, England.
M K
MK
Robinson
D
D
Andrew
H
H
Rosen
D
D
Brown
S
S
Ortlepp
P
P
Stephens
E C
EC
Butcher
D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology*; D015703:Antigens, CD / Q000502:physiology*; D018821:CD18 Antigens; D002448:Cell Adhesion; D002449:Cell Aggregation; D000939:Epitopes / Q000032:analysis; D006801:Humans; D016169:Lymphocyte Function-Associated Antigen-1 / Q000032:analysis / Q000502:physiology*; D016177:Macrophage-1 Antigen / Q000032:analysis / Q000502:physiology*; D009504:Neutrophils / Q000502:physiology; D011487:Protein Conformation
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D018821:CD18 Antigens; D000939:Epitopes; D016169:Lymphocyte Function-Associated Antigen-1; D016177:Macrophage-1 Antigen