Selective determination of L-lysine with L-lysine epsilon-dehydrogenase.
Simple and sensitive spectrophotometric methods were developed for the selective determination of L-lysine. The assay methods were based on the spectrophotometric determination of NADH formed in the reaction catalyzed by L-lysine epsilon-dehydrogenase (Procedure A) or the formazan produced on coupling of the dehydrogenase reaction with the reduction of a tetrazolium salt (Procedure B). Procedure B was applicable to the direct determination of L-lysine in rabbit serum.
Department of Agricultural Chemistry, Kochi University, Japan.
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Hashimoto
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Misono
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Nagata
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Nagasaki
D000594:Amino Acid Oxidoreductases / Q000378:metabolism*; D000818:Animals; D003124:Colorimetry / Q000379:methods; D007202:Indicators and Reagents; D007700:Kinetics; D008239:Lysine / Q000032:analysis* / Q000097:blood; D009243:NAD / Q000032:analysis; D011817:Rabbits; D013056:Spectrophotometry, Ultraviolet / Q000379:methods
D007202:Indicators and Reagents; D009243:NAD; D000594:Amino Acid Oxidoreductases; C034168:lysine epsilon-dehydrogenase; D008239:Lysine