Formation of nitric oxide hemoglobin in erythrocytes co-cultured with alveolar macrophages taken from bleomycin treated rats.
Alveolar macrophages, taken from rats treated with a single intratracheal dose of bleomycin, release reactive nitrogen intermediates in the form of nitric oxide which are cytostatic to murine leukemia L1210 cells. When cultured in the presence of erythrocytes the cytostatic activity of alveolar macrophages was inhibited which corresponded with an increase in nitrosylated hemoglobin content when compared with erythrocytes cultured alone. These results suggest that erythrocytes inhibit alveolar macrophage cytostatic activity by preventing reactive nitrogen intermediates from reaching target cells because the hemoglobin serves as a sink for reactive nitrogen intermediates in the form of nitric oxide.
Huot
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Vermont Regional Cancer Center, University of Vermont, Burlington 05405.
Kruszyna
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Kruszyna
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Smith
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Hacker
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MP
D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives / Q000494:pharmacology; D001761:Bleomycin / Q000494:pharmacology*; D002450:Cell Communication; D002478:Cells, Cultured; D004912:Erythrocytes / Q000166:cytology / Q000502:physiology*; D006454:Hemoglobins / Q000378:metabolism*; D007249:Inflammation; D007939:Leukemia L1210 / Q000473:pathology; D016676:Macrophages, Alveolar / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D008297:Male; D051379:Mice; D009569:Nitric Oxide / Q000378:metabolism*; D009573:Nitrites / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013053:Spectrophotometry; D019323:omega-N-Methylarginine
D006454:Hemoglobins; D009573:Nitrites; C008657:nitrosyl hemoglobin; D001761:Bleomycin; D019323:omega-N-Methylarginine; D009569:Nitric Oxide; D001120:Arginine