Flow cytometric study of hybridoma cell culture: correlation between cell surface fluorescence and IgG production rate.
Flow cytometry was applied to measure the fluorescence of IgG molecules on hybridoma cell surface stained with FITC-conjugated anti-mouse IgG F(ab')2. Using light scattering to exclude the dead cell population from the analysis, the surface fluorescence intensity allows for the differentiation between producing and nonproducing cells. A linear correlation has been found between the intensity of mean surface fluorescence of the cell population and the specific antibody production rate.
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Department of Chemical Engineering and Materials Science, University of Minnesota, Minneapolis 55455.
Hu
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WS
Srienc
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D000818:Animals; D002455:Cell Division; D002460:Cell Line; D002462:Cell Membrane / Q000276:immunology; D005434:Flow Cytometry / Q000379:methods; D006825:Hybridomas / Q000166:cytology / Q000276:immunology*; D007074:Immunoglobulin G / Q000032:analysis / Q000096:biosynthesis*; D007700:Kinetics; D051379:Mice; D013050:Spectrometry, Fluorescence / Q000379:methods
D007074:Immunoglobulin G