HIV-1 gene rev responsible for accumulated heterodisperse RNAs seen in infected T-cells.
To analyze which gene of HIV-1 was responsible for the high accumulation of "heterodisperse RNAs" in virally infected T-cells, Northern blot analysis was performed. Total RNA from inducible cell lines expressing the envelope protein of HIV-1, or the regulatory gene rev, were blotted and hybridized with an Alu probe that detects heterodisperse RNAs. Results show that the rev gene is responsible for the accumulation of cellular heterodisperse RNAs seen earlier in HIV-1-infected T-cells.
Lindström
E
E
Department of Immunology, Karolinska Institute, Stockholm, Sweden.
Koga
Y
Y
Wigzell
H
H
D015152:Blotting, Northern; D002478:Cells, Cultured; D015694:Gene Products, rev / Q000502:physiology*; D015757:Genes, rev; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D066298:In Vitro Techniques; D008970:Molecular Weight; D012313:RNA / Q000737:chemistry / Q000378:metabolism*; D012091:Repetitive Sequences, Nucleic Acid; D013601:T-Lymphocytes / Q000382:microbiology*; D054321:rev Gene Products, Human Immunodeficiency Virus
D015694:Gene Products, rev; D054321:rev Gene Products, Human Immunodeficiency Virus; D012313:RNA