Properties of redox-inactivated bleomycins. In vitro DNA damage and inhibition of Ehrlich cell proliferation.
Blenoxane, bleomycin A2, bleomycin B2, and demethyl bleomycin A2 and products of their reactions with Fe2+ and oxygen were used to explore the relationship between their capacity to carry out in vitro DNA strand scission and their growth inhibitory activity against Ehrlich cells. Reaction of Fe2+, bleomycin and O2 in the absence of DNA decreased the subsequent effectiveness of various bleomycin congeners to degrade DNA in the presence of Fe2+ and oxygen. In comparison with controls, this loss of strand scission activity was not paralleled by equivalent decreases in growth inhibition. Demethyl bleomycin A2 retained full biological activity relative to bleomycin A2, despite being only 30% as effective as bleomycin A2 in its ability to cleave DNA in vitro. Prior reaction of bleomycins with Fe2+ did not alter their capacity to reduce oxygen or affect their ability to generate the activated intermediate which, for native bleomycin structures, is competent to cleave DNA in vitro.
Templin
J
J
Department of Chemistry, University of Wisconsin-Milwaukee 53201.
Berry
L
L
Lyman
S
S
Byrnes
R W
RW
Antholine
W E
WE
Petering
D H
DH
D000818:Animals; D001761:Bleomycin / Q000737:chemistry / Q000494:pharmacology*; D002286:Carcinoma, Ehrlich Tumor; D002455:Cell Division / Q000187:drug effects; D004249:DNA Damage; D008315:Malondialdehyde / Q000032:analysis; D008956:Models, Chemical; D016877:Oxidants; D010084:Oxidation-Reduction; D010101:Oxygen Consumption; D014407:Tumor Cells, Cultured / Q000187:drug effects*
D016877:Oxidants; D001761:Bleomycin; D008315:Malondialdehyde