Isolation of a novel siderophore from Pseudomonas cepacia.
A novel iron-binding compound was identified in ethyl acetate extracts of the supernates from Pseudomonas cepacia cultures. This compound, named azurechelin, was produced by 88% of P. cepacia strains isolated from the respiratory tract. Production of azurechelin was regulated by the iron concentration in the culture medium. Azurechelin enhanced the growth of P. cepacia in a medium containing transferrin 200 mg/L. Azurechelin released iron from transferrin in an equilibrium dialysis assay, suggesting that it could complete with transferrin for iron. Azurechelin could also stimulate iron uptake by P. cepacia. This siderophore appeared to have a novel structure with neither the typical characteristics of catechol nor of hydroxamate compounds.
Department of Microbiology and Infectious Diseases, University of Calgary Health Sciences Centre, Alberta, Canada.
P A
PA
Sokol
C J
CJ
Lewis
J J
JJ
Dennis
D001692:Biological Transport / Q000187:drug effects; D016956:Burkholderia cepacia / Q000737:chemistry* / Q000187:drug effects / Q000254:growth & development; D002850:Chromatography, Gel; D002855:Chromatography, Thin Layer; D003470:Culture Media; D006801:Humans; D007476:Ionophores / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D007501:Iron / Q000378:metabolism / Q000494:pharmacology; D007502:Iron Chelating Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D010636:Phenols / Q000494:pharmacology; D012459:Salicylates; D017262:Siderophores; D013844:Thiazoles
D003470:Culture Media; D007476:Ionophores; D007502:Iron Chelating Agents; D010636:Phenols; D012459:Salicylates; D017262:Siderophores; D013844:Thiazoles; C073703:azurechelin; C025316:pyochelin; D007501:Iron