The effects of burn injury on the acute phase response.
The time course of changes in the levels of acute-phase-reactant (APR) mRNAs in different tissues of rats with a 10% or a 60% total-body-surface-area (TBSA) burn and the relationship between the induction of APRs and the host's tolerance to thermal injury were studied. The acute phase response in a LPS-induced inflammation model and a burn-plus-LPS model were compared. The results of this study indicated that (1) the major site of APR synthesis is the liver; (2) even a small surface burn injury can elicit a rapid acute phase response, but the intensity of APR expression increases with the severity of the burn; (3) the down regulation of albumin mRNA, which is characteristic of the acute phase response, does not occur even though transferrin (Trf) mRNA levels are significantly decreased; (4) the resistant strain of inbred rats showed higher levels of alpha 1-antitrypsin (AT) mRNA before and after burn injury, indicating its contribution to the host's tolerance to thermal injury; (5) the increases in alpha 1-acid glycoprotein (AGP) and AT expressions are limited in the burn-plus-LPS rat model compared with either the burn model or LPS-stimulated model alone.
Shriners Burn Institute, Galveston, Texas.
Z F
ZF
Xia
M I
MI
Coolbaugh
F
F
He
D N
DN
Herndon
J
J
Papaconstantinou
D000209:Acute-Phase Proteins / Q000235:genetics / Q000378:metabolism*; D000418:Albumins / Q000378:metabolism; D000818:Animals; D002056:Burns / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D007249:Inflammation / Q000150:complications / Q000378:metabolism; D008070:Lipopolysaccharides / Q000008:administration & dosage; D008099:Liver / Q000378:metabolism; D008297:Male; D009961:Orosomucoid / Q000235:genetics / Q000378:metabolism; D011550:Pseudomonas aeruginosa; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D011915:Rats, Inbred BUF; D011916:Rats, Inbred F344; D011919:Rats, Inbred Strains; D014168:Transferrin / Q000378:metabolism; D000515:alpha 1-Antitrypsin / Q000235:genetics / Q000378:metabolism
D000209:Acute-Phase Proteins; D000418:Albumins; D008070:Lipopolysaccharides; D009961:Orosomucoid; D012333:RNA, Messenger; D014168:Transferrin; D000515:alpha 1-Antitrypsin