Characterization of the human 5-hydroxytryptamine1B receptor.
We report the cloning of a human gene encoding the 5-hydroxytryptamine1B receptor. The receptor has the characteristics of a G-protein-linked receptor and is most homologous to the human 5-HT1D receptor. This human 5-HT receptor gene, most abundantly expressed in striatum, is localized on chromosome 6, at 6q13, and the gene encoding the 5-HT1D receptor is localized on chromosome 1. Radioligand studies indicate that the affinity of [3H]5-HT is 16 +/- 2 nM. Drug competition studies indicate that the receptor displays high affinity (i.e. less than 40 nM) for 5-HT, 5-carboxyamidotryptamine, methiothepin, and metergoline.
Jin
H
H
Department of Pharmacology, University of Toronto, Ontario, Canada.
Oksenberg
D
D
Ashkenazi
A
A
Peroutka
S J
SJ
Duncan
A M
AM
Rozmahel
R
R
Yang
Y
Y
Mengod
G
G
Palacios
J M
JM
O'Dowd
B F
BF
D000595:Amino Acid Sequence; D000818:Animals; D001345:Autoradiography; D001483:Base Sequence; D001667:Binding, Competitive; D015152:Blotting, Northern; D015139:Blotting, Southern; D001921:Brain / Q000378:metabolism*; D002896:Chromosomes, Human, Pair 6; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D015698:Genomic Library; D006801:Humans; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D009928:Organ Specificity; D010902:Pituitary Gland / Q000378:metabolism*; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification; D051381:Rats; D011985:Receptors, Serotonin / Q000032:analysis / Q000235:genetics* / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D015183:Restriction Mapping; D012701:Serotonin / Q000378:metabolism; D014316:Tritium
D009838:Oligodeoxyribonucleotides; D012333:RNA, Messenger; D011985:Receptors, Serotonin; D011994:Recombinant Proteins; D014316:Tritium; D012701:Serotonin; D004247:DNA