Assay for N-acetylmuramyl-L-alanine amidase in serum by determination of muramic acid released from the peptidoglycan of Brevibacterium divaricatum.
A method is reported for the determination of N-acetylmuramyl-L-alanine amidase in serum. Muramic acid, released from the interpeptide bridges of Brevibacterium divaricatum peptidoglycan, is measured by a modified colorimetric method. Using this procedure, it was possible to determine N-acetylmuramyl-L-alanine amidase in aliquots of less than 10 microliters human serum with an incubation time of 10 min. Amidase activity was found in all the sera tested (n = 11). The relevance of this simple and fast assay is discussed.
Department of Immunology, Erasmus University Rotterdam, The Netherlands.
M P
MP
Hazenberg
H
H
de Visser
D000328:Adult; D001774:Blood Chemical Analysis / Q000379:methods; D001951:Brevibacterium; D003124:Colorimetry / Q000379:methods; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009112:Muramic Acids / Q000032:analysis; D009238:N-Acetylmuramoyl-L-alanine Amidase / Q000097:blood*; D010457:Peptidoglycan; D013379:Substrate Specificity
D009112:Muramic Acids; D010457:Peptidoglycan; D009238:N-Acetylmuramoyl-L-alanine Amidase