[Cholecalciferol Reference Standard (Control 901) of the National Institute of Hygienic Sciences].
Cholecalciferol Reference Standard for the Japanese Pharmacopoeia (JP RS) was prepared. The following analytical data were obtained: melting point 85.8 degrees C; UV and IR spectra were in agreement with both of the previous JP RS and USP RS of Cholecalciferol; absorptivity at 265 nm E1%lcm = 476; optical rotation [alpha]20D = + 110.1 degrees; no impurities were detected by TLC and HPLC analyses; assay 100.1% by HPLC against the USP RS. Based on the above results, the raw material was authorized as the Reference Standard of the National Institute of Hygienic Sciences.
H
H
Kakehi
H
H
Komatsu
M
M
Tanaka
M
M
Murai
S
S
Ishimitsu
S
S
Okada
D002762:Cholecalciferol / Q000302:isolation & purification / Q000592:standards*; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D006077:Government Agencies; D006920:Hygiene; D007564:Japan; D010602:Pharmacopoeias as Topic / Q000592:standards*
D002762:Cholecalciferol