The separation and identification of picomole amounts of intermediates of glucose metabolism by high performance liquid chromatography on pellicular resins.
A column (CarboPac PA1, Dionex) containing an anion-exchange pellicular resin was used for the separation of phosphoryl-hexoses derived from labeled glucose microinjected into individual frog oocytes or from cultures of Escherichia coli. Intermediates were identified by: a) comparison of retention times with those of authentic commercial compounds; b) the use of internal labeled standards; c) incubation of samples with specific enzymes and noting the disappearance of one radioactive peak and appearance of another at a new retention time.
Departamento de Biología, Facultad de Ciencias, Universidad de Chile, Santiago.
A
A
Preller
V
V
Guixe
J C
JC
Torres
H W
HW
Hofer
T
T
Ureta
D000818:Animals; D001001:Anura; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004926:Escherichia coli / Q000378:metabolism; D005260:Female; D005947:Glucose / Q000378:metabolism*; D006601:Hexoses / Q000302:isolation & purification; D008845:Microinjections; D009865:Oocytes / Q000378:metabolism; D012116:Resins, Plant; D013997:Time Factors
D006601:Hexoses; D012116:Resins, Plant; D005947:Glucose