Transglutaminase catalyses the modification of glutamine side chains in the C-terminal region of bovine beta-lactoglobulin.
The transglutaminase-catalysed incorporation of primary amines (putrescine and monodansylcadaverine) into bovine beta-lactoglobulin has been studied. In the presence of 1 mM-dithiothreitol between 1 and 2 mol of amine can be incorporated per mol of beta-lactoglobulin subunit. There is very little incorporation of amines in the absence of reducing agent. By isolating and sequencing the modified peptides, the sites of modification have been identified as Gln-159 (preferred) and Gln-155. C.d. has been used to study the structure of beta-lactoglobulin over a range of pH values and in the presence or absence of dithiothreitol. The results are discussed in terms of the X-ray-crystallographically determined structure of beta-lactoglobulin.
Department of Biological and Molecular Sciences, University of Stirling, Scotland, U.K.
P J
PJ
Coussons
N C
NC
Price
S M
SM
Kelly
B
B
Smith
L
L
Sawyer
D000595:Amino Acid Sequence; D000818:Animals; D002103:Cadaverine / Q000031:analogs & derivatives / Q000378:metabolism; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D002942:Circular Dichroism; D004229:Dithiothreitol / Q000494:pharmacology; D005973:Glutamine / Q000378:metabolism*; D006168:Guinea Pigs; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D007782:Lactoglobulins / Q000737:chemistry / Q000378:metabolism*; D008099:Liver / Q000201:enzymology*; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism; D011487:Protein Conformation; D011700:Putrescine / Q000378:metabolism; D011503:Transglutaminases / Q000378:metabolism*
D007782:Lactoglobulins; D010446:Peptide Fragments; D005973:Glutamine; D011503:Transglutaminases; C008542:monodansylcadaverine; D002103:Cadaverine; D004229:Dithiothreitol; D011700:Putrescine