Immunologically active polysaccharides of Arnica montana cell cultures.
From the nutrition medium of Arnica montana cell cultures two homogeneous polysaccharides, an acidic arabino-3,6-galactan-protein with mean Mr of 100,000 and a neutral fucogalactoxyloglucan with mean Mr of 22,500 have been isolated by DEAE-Sepharose CL-6B and Sephacryl S-400 column chromatography. Their structures were elucidated mainly by methylation analysis, partial acidic and enzymatic hydrolysis and 13C NMR spectroscopy. The fucogalactoxyloglucan shows a pronounced enhancement of phagocytosis in vivo. The arabino-3,6-galactan-protein displays a strong anticomplementary effect and stimulates macrophages to excrete the tumour necrosis factor (TNF alpha).
Institute of Pharmaceutical Biology, University of Munich, F.R.G.
J
J
Puhlmann
M H
MH
Zenk
H
H
Wagner
D002240:Carbohydrate Sequence; D002478:Cells, Cultured; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D008969:Molecular Sequence Data; D015394:Molecular Structure; D010944:Plants / Q000032:analysis / Q000276:immunology; D011134:Polysaccharides / Q000276:immunology* / Q000302:isolation & purification
D011134:Polysaccharides