Reductive dechlorination of 1,2-dichloroethane and chloroethane by cell suspensions of methanogenic bacteria.
Concentrated cell suspensions of methanogenic bacteria reductively dechlorinated 1,2-dichloroethane via two reaction-mechanisms: a dihalo-elimination yielding ethylene and two hydrogenolysis reactions yielding chloroethane and ethane, consecutively. The transformation of chloroethane to ethane was inhibited by 1,2-dichloroethane. Stimulation of methanogenesis caused an increase in the amount of dechlorination products formed, whereas the opposite was found when methane formation was inhibited. Cells of Methanosarcina barkeri grown on H2/CO2 converted 1,2-dichloroethane and chloroethane at higher rates than acetate or methanol grown cells.
Department of Microbiology, Wageningen Agricultural University, The Netherlands.
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C
Holliger
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Schraa
A J
AJ
Stams
A J
AJ
Zehnder
D001673:Biodegradation, Environmental; D002713:Chlorine / Q000378:metabolism; D005018:Ethyl Chloride / Q000378:metabolism*; D005025:Ethylene Dichlorides / Q000378:metabolism*; D019605:Euryarchaeota / Q000378:metabolism*; D017014:Methanobacterium / Q000378:metabolism; D017017:Methanococcus / Q000378:metabolism; D017021:Methanosarcina barkeri / Q000378:metabolism; D010084:Oxidation-Reduction; D014874:Water Pollutants, Chemical / Q000378:metabolism
D005025:Ethylene Dichlorides; D014874:Water Pollutants, Chemical; D005018:Ethyl Chloride; D002713:Chlorine; C024565:ethylene dichloride