In vitro and in vivo detection of somatostatin receptors in human malignant lymphomas.
A wide variety of primary and metastatic human neoplasms express somatostatin receptors (SS-Rs). We evaluated the SS-R status of malignant lymphomas that had been surgically removed from 31 patients by use of in vitro SS-R autoradiography with the SS analog 125I-[Tyr3]-octreotide as radioligand. Of 11 low-grade-malignancy B-cell non-Hodgkin's lymphomas, 10 were SS-R-positive, with a high receptor density restricted to the neoplastic follicles. All of the 8 intermediate-grade lymphomas were SS-R-positive. Of the B-cell lymphomas of high-grade malignancy, 7 out of 10 were SS-R-positive, often with a high density of receptors. One T-cell lymphoma and one Hodgkin's lymphoma were also positive. SS-Rs were of high affinity (KD = 1.2 nM) and specific for bioactive SS analogs. In 4 patients, the lymphomas were localized in vivo by use of gamma-camera scintigraphy after i.v. injection of the SS analog 111In-[DTPA-D-Phe1]-octreotide. Hot spots, identified in all 4 patients, corresponded to SS-R-positive malignant-lymphoma tissue, as confirmed by receptor autoradiography of the surgically removed tumors. Our data show that SS-Rs are valuable pathobiochemical tissue markers and potentially useful in vivo diagnostic tools for human malignant lymphomas.
Sandoz Research Institute, Berne, Switzerland.
J C
JC
Reubi
B
B
Waser
M
M
van Hagen
S W
SW
Lamberts
E P
EP
Krenning
J O
JO
Gebbers
J A
JA
Laissue
D000293:Adolescent; D000328:Adult; D000368:Aged; D001345:Autoradiography; D001706:Biopsy; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007205:Indium Radioisotopes; D007457:Iodine Radioisotopes; D007700:Kinetics; D008223:Lymphoma / Q000000981:diagnostic imaging* / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D015282:Octreotide / Q000031:analogs & derivatives* / Q000378:metabolism; D004369:Pentetic Acid / Q000031:analogs & derivatives* / Q000378:metabolism; D011877:Radionuclide Imaging; D017981:Receptors, Neurotransmitter / Q000032:analysis* / Q000378:metabolism; D017481:Receptors, Somatostatin; D013004:Somatostatin / Q000378:metabolism
D007205:Indium Radioisotopes; D007457:Iodine Radioisotopes; D017981:Receptors, Neurotransmitter; D017481:Receptors, Somatostatin; C045210:3-Tyr-octreotide; C071632:SDZ 215-811; D013004:Somatostatin; D004369:Pentetic Acid; D015282:Octreotide