Assignment of genes encoding a unique cytokine (IL12) composed of two unrelated subunits to chromosomes 3 and 5.
IL12 (formerly NKSF or CLMF) is a unique cytokine composed of two unrelated disulfide-linked subunits. The larger 40-kDa subunit (p40) is a member of the cytokine receptor family, and the smaller 35-kDa subunit (p35) is related to IL6 and GCSF. The chromosomal localization of these two subunits was determined by PCR analysis of DNA from rodent-human hybrids. More refined mapping was obtained by PCR analysis of hybrids containing translocation chromosomes and for p40, by analysis of radiation hybrids. The subunits map to different chromosomes: p40 (IL12B) to 5q31-q33 and p35 (IL12A) to 3p12-3q13.2.
Sieburth
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Genetic Institute, Cambridge, Massachusetts 02140.
Jabs
E W
EW
Warrington
J A
JA
Li
X
X
Lasota
J
J
LaForgia
S
S
Kelleher
K
K
Huebner
K
K
Wasmuth
J J
JJ
Wolf
S F
SF
D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002893:Chromosomes, Human, Pair 3; D002895:Chromosomes, Human, Pair 5; D006224:Cricetinae; D004247:DNA / Q000032:analysis; D006801:Humans; D006822:Hybrid Cells; D018664:Interleukin-12; D007378:Interleukins / Q000235:genetics*; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction
D007378:Interleukins; D015345:Oligonucleotide Probes; D018664:Interleukin-12; D004247:DNA