Identification of Mycoplasma hyopneumoniae with a DNA probe.
From a genomic library of Mycoplasma hyopneumoniae a 1.3 kb DNA fragment was cloned which showed specific Southern hybridization and dot hybridization with the type strain of several porcine and bovine Mycoplasma species. This probe selectively recognized M. hyopneumoniae sequences in purified DNA or in broth-grown organisms. The 35S-labelled probe could detect as little as 100 pg of DNA or 10(5) colour changing units. This is a possible alternative diagnostic procedure for enzootic pneumonia of pigs.
National Veterinary Laboratory, Copenhagen, Denmark.
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P
Ahrens
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NF
Friis
D000818:Animals; D015139:Blotting, Southern; D015342:DNA Probes; D004269:DNA, Bacterial / Q000032:analysis*; D015723:Gene Library; D008168:Lung / Q000382:microbiology; D009174:Mycoplasma / Q000235:genetics / Q000302:isolation & purification*; D009693:Nucleic Acid Hybridization; D045729:Pneumonia of Swine, Mycoplasmal / Q000175:diagnosis / Q000662:veterinary*; D011237:Predictive Value of Tests; D013047:Specific Pathogen-Free Organisms; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis*
D015342:DNA Probes; D004269:DNA, Bacterial