Nucleotide sequence of the gene coding for the BanIII DNA methyltransferase in Bacillus aneurinolyticus.
The gene coding for the ATCGAT specific BanIII DNA methyltransferase (M-BanIII) of Bacillus aneurinolyticus was cloned and its nucleotides sequenced. The coding region was assigned on the nucleotide sequence on the basis of the N-terminal amino acid sequence and molecular weight of the enzyme. The M-BanIII gene coded for a protein of 580 amino acid residues (MW 66,344). Comparison with other methylases indicated that the M-BanIII sequence contained a segment of tetra-amino acids, NPPY, characteristic of N6-adenine methylases. In addition, some homologous regions were found in the sequences of type II adenine methylases PaeR7I(CTCGAG), TaqI(TCGA) and PstI(CTGCAG), containing TCGA within the recognition sequences.
Tsuruga Enzyme Plant, Toyobo Co., Ltd., Fukui, Japan.
B
B
Kawakami
A
A
Sasaki
M
M
Oka
Y
Y
Maekawa
D000595:Amino Acid Sequence; D001407:Bacillus / Q000201:enzymology* / Q000235:genetics; D001483:Base Sequence; D003470:Culture Media; D004269:DNA, Bacterial / Q000235:genetics; D008969:Molecular Sequence Data; D008970:Molecular Weight; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific) / Q000096:biosynthesis / Q000235:genetics*
D003470:Culture Media; D004269:DNA, Bacterial; C061926:DNA modification methylase Cla I; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific)