Recombinant TSH-receptor for determination of TSH-receptor-antibodies.
We have expressed the complete coding sequence of the human TSH-R in eucaryotic cells. Limiting dilution enabled us to select two clones, JP09 and JP26, which have formed the basis of binding and bioassays respectively, for TSH-R antibodies. Results obtained in the binding assay correlated well with those obtained in the TRAK (Henning Berlin) assay, while the bioassay was at least as sensitive as measurements made with FRTL5 or human thyroid cells. These cell lines provide a reliable source of human TSH-R which will be useful in routine diagnosis.
Institut de Recherche Interdisciplinaire, Université Libre de Bruxelles/Belgium.
M
M
Ludgate
S
S
Costagliola
D
D
Danguy
J
J
Perret
G
G
Vassart
D000818:Animals; D001323:Autoantibodies / Q000032:analysis* / Q000378:metabolism; D001681:Biological Assay; D016466:CHO Cells; D003001:Cloning, Molecular; D006224:Cricetinae; D000242:Cyclic AMP / Q000378:metabolism; D006111:Graves Disease / Q000276:immunology; D006801:Humans; D018828:Immunoglobulins, Thyroid-Stimulating; D011989:Receptors, Thyrotropin / Q000235:genetics / Q000276:immunology*; D011994:Recombinant Proteins / Q000276:immunology*; D013972:Thyrotropin / Q000378:metabolism / Q000494:pharmacology; D014162:Transfection
D001323:Autoantibodies; D018828:Immunoglobulins, Thyroid-Stimulating; D011989:Receptors, Thyrotropin; D011994:Recombinant Proteins; D013972:Thyrotropin; D000242:Cyclic AMP