Mapping of ornithine aminotransferase gene sequences to mouse chromosomes 7, X, and 3.
Ornithine aminotransferase (OAT), a mitochondrial matrix enzyme, is deficient in patients with gyrate atrophy of the choroid and retina. In human, the OAT structural gene maps to Chromosome (Chr) 10q26 and several OAT-related sequences, some of which are known to be processed pseudogenes, which map to Xp11.3-11.21. Here, we report chromosomal localization in the mouse of the OAT gene and related sequences. Genomic DNA blot analysis of a well-characterized panel of Chinese hamster x mouse somatic cell hybrids using a human OAT probe revealed two murine loci, one on mouse Chr 7 and the other on Chr X. In addition, segregation of restriction fragment length polymorphisms (RFLPs) detected by the OAT probe in recombinant inbred (RI) strains detected a third locus on Chr 3 and positioned the X locus near Cf-8 and Rsvp. Progeny of an intersubspecific backcross were used to map the Chr 7 locus between Tyr and Int-2, near Cyp2e-1.
Molecular Neurogenetics Laboratory, Massachusetts General Hospital, Charlestown 02129.
V
V
Ramesh
S V
SV
Cheng
C A
CA
Kozak
B J
BJ
Herron
V E
VE
Shih
B A
BA
Taylor
J F
JF
Gusella
D000483:Alleles; D000818:Animals; D002874:Chromosome Mapping; D006224:Cricetinae; D003412:Cricetulus; D003433:Crosses, Genetic; D004262:DNA Restriction Enzymes; D005260:Female; D006822:Hybrid Cells; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009953:Ornithine-Oxo-Acid Transaminase / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D011995:Recombination, Genetic / Q000235:genetics
D009953:Ornithine-Oxo-Acid Transaminase; D004262:DNA Restriction Enzymes