NADPH regeneration by glucose dehydrogenase from Gluconobacter scleroides for l-leucovorin synthesis.
A new process for (6S)-tetrahydrofolate production from dihydrofolate was designed that used dihydrofolate reductase and an NADPH regeneration system. Glucose dehydrogenase from Gluconobacter scleroides KY3613 was used for recycling of the cofactor. The reaction mixture contained 200 mM dihydrofolate, 220 mM glucose, 2 mM NADP, 14.4 U/ml dihydrofolate reductase, and 14.4 U/ml Glucose dehydrogenase, and the reaction was complete after incubation at pH 8.0, and 40 degrees C for 2.5 hr. With (6S)-tetrahydrofolate as the starting material, l-leucovorin was synthesized via a methenyl derivative. The purity of the l-leucovorin was 100%, and its diastereomeric purity was greater than 99.5% d.e. as the (6S)-form.
Eguchi
T
T
Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., Tokyo, Japan.
Kuge
Y
Y
Inoue
K
K
Yoshikawa
N
N
Mochida
K
K
Uwajima
T
T
D005492:Folic Acid / Q000031:analogs & derivatives / Q000378:metabolism; D042843:Glucose 1-Dehydrogenase; D005948:Glucose Dehydrogenases / Q000378:metabolism*; D002955:Leucovorin / Q000096:biosynthesis*; D009249:NADP / Q000378:metabolism*; D011548:Pseudomonadaceae / Q000201:enzymology*; D013762:Tetrahydrofolate Dehydrogenase / Q000378:metabolism; D013763:Tetrahydrofolates / Q000378:metabolism
D013763:Tetrahydrofolates; C010920:dihydrofolate; C030371:5,6,7,8-tetrahydrofolic acid; D009249:NADP; D005492:Folic Acid; D005948:Glucose Dehydrogenases; D042843:Glucose 1-Dehydrogenase; D013762:Tetrahydrofolate Dehydrogenase; D002955:Leucovorin