Dopa accumulates in the hypothalamic-hypophysial portal vessels and is taken into the anterior pituitary of NSD-1015-treated rodents.
DOPA was measured in the anterior pituitary and hypothalamic-hypophysial portal blood after treatment with NSD-1015, a DOPA decarboxylase inhibitor. NSD-1015 caused DOPA to accumulate in the anterior pituitary of mice and rats, and increased DOPA in the hypothalamic-hypophysial portal blood of rat. Serum prolactin was also increased. Interruption of the anterior pituitary blood supply from the hypothalamic-hypophysial system by cannulation of the entire pituitary stalk eliminated the NSD-1015-induced DOPA accumulation in the rat pituitary. We conclude that DOPA can be taken into the anterior pituitary from the portal blood of NSD-1015-treated rodents and that the anterior pituitary lacks tyrosine hydroxylase activity in both mice and rats.
Andrus Gerontology Center, Department of Biological Sciences, University of Southern California, Los Angeles.
N
N
Telford
P C
PC
May
Y N
YN
Sinha
J C
JC
Porter
C E
CE
Finch
D000818:Animals; D065105:Aromatic Amino Acid Decarboxylase Inhibitors; D002851:Chromatography, High Pressure Liquid; D004295:Dihydroxyphenylalanine / Q000097:blood*; D005260:Female; D006834:Hydrazines / Q000494:pharmacology*; D007031:Hypothalamus / Q000098:blood supply*; D051379:Mice; D008810:Mice, Inbred C57BL; D010902:Pituitary Gland / Q000098:blood supply*; D010903:Pituitary Gland, Anterior / Q000378:metabolism*; D011168:Portal System; D011388:Prolactin / Q000097:blood; D051381:Rats; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
D065105:Aromatic Amino Acid Decarboxylase Inhibitors; D006834:Hydrazines; D004295:Dihydroxyphenylalanine; D011388:Prolactin; C004297:3-hydroxybenzylhydrazine; D014446:Tyrosine 3-Monooxygenase