pIF21, a versatile donor of transposons Tn1, Tn5 and Tn7 for tagging and mobilizing cryptic and non-selectable plasmids.
A plasmid, pIF21, has been constructed that is able to donate transposons Tn1, Tn5 and Tn7. The transposons are located on a temperature-sensitive derivative of the incP1 plasmid pRP1, which is transferable to a wide range of Gram-negative genera.
Feavers
I M
IM
Department of Microbiology, Medical School, Newcastle upon Tyne, UK.
Fife
A J
AJ
Harwood
C R
CR
D003227:Conjugation, Genetic; D004251:DNA Transposable Elements; D006090:Gram-Negative Bacteria / Q000235:genetics*; D010957:Plasmids
D004251:DNA Transposable Elements