Construction of Salmonella strains with both antigen O4 (of group B) and antigen O9 (of group D).
A Salmonella live vaccine causing both O4- and O9-specific immune responses would be of use, but no reported Salmonella serotype has both of these O antigens. Constructed Salmonella typhimurium strains with an rfb (O-antigen-specifying) gene cluster of type D in the chromosome and one of type B in an F'-rfb+ factor, and those with the reverse combination reacted strongly with both anti-O4 (and anti-O5) and anti-O9 sera and, if they carried recA, could be maintained in this state by growth conditions selective for retention of the F' factor. One of the two B.rfb+ gene clusters of a (P22-lysogenic) S. typhimurium strain with a tandem duplication of a chromosomal segment including hisD and B.rfb+ was replaced (by transduction) by a D.rfb+ gene cluster; the resulting strain was O1+ O4+ O5+ O9+ and stable as such after being made recA. A stable O4+ O9+ derivative of a virulent S. enteritidis (O-group D) strain was made by transducing into it first the join point of an appropriate tandem duplication strain, together with the adjacent B.rfb+ gene cluster, and then srl::Tn10 recA.
Department of Microbiology and Immunology, Stanford University School of Medicine, California 94305.
B N
BN
Johnson
A
A
Weintraub
A A
AA
Lindberg
B A
BA
Stocker
D003001:Cloning, Molecular; D005144:F Factor; D005798:Genes, Bacterial; D008070:Lipopolysaccharides / Q000737:chemistry; D019081:O Antigens; D011135:Polysaccharides, Bacterial / Q000235:genetics* / Q000276:immunology*; D012477:Salmonella enteritidis / Q000235:genetics / Q000276:immunology*; D012486:Salmonella typhimurium / Q000235:genetics / Q000276:immunology*
D008070:Lipopolysaccharides; D019081:O Antigens; D011135:Polysaccharides, Bacterial