Human gastric cathepsin E gene. Multiple transcripts result from alternative polyadenylation of the primary transcripts of a single gene locus at 1q31-q32.
Genomic clones containing portions of the human cathepsin E (CTSE) gene were isolated from cosmid and lambda recombinant libraries. The regions corresponding to coding, the 5'- and 3'-untranslated, and the exon-intron boundaries of the CTSE gene were identified by sequence and hybridization analysis. The size and placement of the nine exons found in the 17.5-kilobase CTSE gene was highly conserved relative to other aspartic proteinases and provided additional evidence that these proteinases are derived from a common ancestral gene. Segregation and linkage analysis of two informative restriction fragment length polymorphisms (MspI and DraI) indicated that there is a single human CTSE locus located at chromosome 1q31-q32 which is closely linked to the renin gene. Three CTSE transcripts (3.6, 2.6, and 2.1 kilobases) were identified in gastric fundic and antral mucosa poly (A+) RNA, and these appeared identical in size and relative abundance to those contained in poly(A+) RNA from cultured gastric adenocarcinoma cell lines containing CTSE. Sequence analysis of cDNA clones and comparison with the 3'-flanking untranslated region in genomic clones provided evidence that alternative polyadenylation of the primary transcript resulted in the 2.6- and 2.1-kilobase transcripts which constituted greater than 95% of CTSE transcripts found in the stomach.
Department of Molecular Biology and Genetics, Wayne State University School of Medicine, Detroit, Michigain 48201.
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T
Azuma
W G
WG
Liu
D J
DJ
Vander Laan
A M
AM
Bowcock
R T
RT
Taggart
D000595:Amino Acid Sequence; D001483:Base Sequence; D020059:Cathepsin E; D002403:Cathepsins / Q000235:genetics*; D002871:Chromosome Banding; D002878:Chromosomes, Human, Pair 1; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D019003:Deoxyribonuclease HpaII; D015252:Deoxyribonucleases, Type II Site-Specific; D005753:Gastric Mucosa / Q000201:enzymology*; D008040:Genetic Linkage; D006801:Humans; D008214:Lymphocytes / Q000502:physiology; D008969:Molecular Sequence Data; D011061:Poly A / Q000235:genetics* / Q000378:metabolism; D012150:Polymorphism, Restriction Fragment Length; D012313:RNA / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger; D012083:Renin / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic
D012333:RNA, Messenger; D011061:Poly A; D012313:RNA; D004247:DNA; D019003:Deoxyribonuclease HpaII; D015252:Deoxyribonucleases, Type II Site-Specific; C035594:TTTAAA -specific type II deoxyribonucleases; D002403:Cathepsins; D012083:Renin; D020059:Cathepsin E