Lineage and stage specific expression of HOX 1 genes in the human hematopoietic system.
Recent observations have demonstrated the expression of several members of the homeobox-containing (HOX) gene complexes within the hematopoietic compartment. We have analyzed the expression pattern of the entire HOX 1 locus in a panel of leukemia-derived human cell lines representing various blood phenotypes. The expression of the eleven HOX 1 genes is lineage-restricted and these genes are predominantly detected within cells of myelomonocytic origin. This is in strong contrast with the erythro-megakaryocytic specific expression of HOX 2 genes. Furthermore, we have observed that the expression of three HOX 1 genes within B lymphoid lineages is stage-related and that the expression of several of them is switched off during TPA-induced differentiation of Kg1 and U937. These observations suggest that HOX 1 homeoproteins could be regulators of lineage determination during hematopoiesis.
INSERM U217-CENG/DBMS/HEM 85X-38041 Grenoble, France.
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Vieille-Grosjean
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Roullot
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Courtois
D001402:B-Lymphocytes / Q000378:metabolism; D002454:Cell Differentiation; D002460:Cell Line; D015870:Gene Expression; D005801:Genes, Homeobox / Q000235:genetics*; D006410:Hematopoiesis / Q000235:genetics*; D006413:Hematopoietic System; D006801:Humans; D007942:Leukemia, Experimental; D015473:Leukemia, Promyelocytic, Acute; D008264:Macrophages / Q000378:metabolism; D016133:Polymerase Chain Reaction; D012322:RNA Precursors / Q000032:analysis
D012322:RNA Precursors