Atrial natriuretic peptide during water deprivation or hemorrhage in rats. Relationship with arginine vasopressin and osmolarity.
The concentrations of atrial natriuretic peptide (ANP) in atria, hypothalami and plasma were investigated in relation to the variations of the plasma endogenous immunoreactive arginine vasopressin (Ir-AVP) during water deprivation or hemorrhage in normal conscious Wistar rats. Furthermore, the in vitro and in vivo effect of extracellular hyperosmolarity on ANP release from right atrium and hypothalamus was examined. Water deprivation elevated circulating immunoreactive ANP (Ir-ANP: pg/ml) to 153 +/- 7 (24 h); 174 +/- 1 (48 h) from the control level (109.6 +/- 7.8). This increase in Ir-ANP concentration which correlated with atrial (r = -0.93) or hypothalamic (r = -0.87) Ir-ANP content decrease, was associated with significantly enhanced levels of plasma Ir-AVP, plasma sodium, osmolarity and hematocrit. An acute volume depletion by hemorrhage significantly reduced plasma Ir-ANP (67 +/- 8.4 pg/ml) from the sham operated level (140 +/- 18 pg/ml). Plasma Ir-AVP was elevated dramatically (207.4 +/- 53.4 pg/ml) compared with the sham operated level (8.8 +/- 2.6 pg/ml). These results, indicating the lack of correlation between plasma Ir-ANP and Ir-AVP in vivo, suggest that the ANP secretion, which is regulated mainly by plasma volume, may be modulated by a change in plasma osmolarity. Extracellular hyperosmolarity stimulated the ANP release from superfused sliced normal rat atria and hypothalami.
Service de Biochimie Médicale, Faculté de Médecine, Pitié-Salpêtrière, Paris, France.
M A
MA
Zongazo
A
A
Carayon
F
F
Masson
R
R
Isnard
J
J
Eurin
G
G
Maistre
C
C
Barthélemy
A C
AC
Prost
J C
JC
Legrand
D000818:Animals; D001127:Arginine Vasopressin / Q000097:blood* / Q000378:metabolism; D009320:Atrial Natriuretic Factor / Q000097:blood* / Q000378:metabolism; D001810:Blood Volume; D002543:Cerebral Hemorrhage / Q000097:blood* / Q000503:physiopathology; D006321:Heart / Q000503:physiopathology; D006325:Heart Atria; D007031:Hypothalamus / Q000378:metabolism*; D008297:Male; D009206:Myocardium / Q000378:metabolism*; D009994:Osmolar Concentration; D011863:Radioimmunoassay; D051381:Rats; D017208:Rats, Wistar; D014868:Water Deprivation
D001127:Arginine Vasopressin; D009320:Atrial Natriuretic Factor