Immunochemical studies and complete amino acid sequence of the streptokinase from Streptococcus pyogenes (group A) M type 12 strain A374.
The complete amino acid sequence of the streptokinase (SKase) of Streptococcus pyogenes M type 12 strain A374, isolated from a patient with poststreptococcal glomerulonephritis (PSGN), was determined. The epitope domain for the monoclonal antibody N-59, which cross-reacts with SKases of both the PSGN-associated strain and S. equisimilis H46A (a non-PSGN-associated strain), was predicted to be localized in residues 370 to 374. The epitope domain specific for monoclonal antibody RU-1, which reacts only with the PSGN-associated SKase, was localized to residues 164 to 236.
Division of Immunology, Nippon Medical School, Kanagawa, Japan.
H
H
Ohkuni
Y
Y
Todome
H
H
Suzuki
M
M
Mizuse
N
N
Kotani
K
K
Horiuchi
N
N
Shikama
A
A
Tsugita
K H
KH
Johnston
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D015153:Blotting, Western; D002874:Chromosome Mapping; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000235:genetics; D008969:Molecular Sequence Data; D012689:Sequence Homology, Nucleic Acid; D013297:Streptococcus pyogenes / Q000201:enzymology*; D013300:Streptokinase / Q000235:genetics* / Q000276:immunology*
D000596:Amino Acids; D000939:Epitopes; D013300:Streptokinase