Comparison of coagulase-negative staphylococci by pulsed-field electrophoresis.
Five pathogenic strains each of Staphylococcus epidermidis, S. haemolyticus, S. lugdunensis and S. schleiferi were analysed by conventional electrophoresis and field inversion gel electrophoresis. For these coagulase-negative staphylococci, the restriction endonuclease SmaI emerged as the most suitable enzyme for pulsed-field electrophoresis by providing an adequate number of clearly separated DNA fragments. Field inversion gel electrophoresis confirmed the differences among strains already discriminated by conventional electrophoresis, and furthermore, differentiated strains which had previously appeared identical. Among the species that were studied, S. epidermidis showed great genomic diversity with a few common bands. On the contrary, S. haemolyticus, S. lugdunensis and S. schleiferi showed less diversity. Although these minor variations may be epidemiologically significant, this question has to be investigated on a larger number of strains.
Laboratoire de Bactériologie, Hôpital Louis Pradel, Lyon, France.
B
B
Lina
F
F
Vandenesch
J
J
Etienne
B
B
Kreiswirth
J
J
Fleurette
D003030:Coagulase / Q000032:analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D016521:Electrophoresis, Gel, Pulsed-Field; D012150:Polymorphism, Restriction Fragment Length; D013210:Staphylococcus / Q000201:enzymology / Q000235:genetics*
D003030:Coagulase; D004269:DNA, Bacterial; D015252:Deoxyribonucleases, Type II Site-Specific