A comparison of an enzymatic and a gas-chromatographic method for measuring the acetate concentration in the blood plasma of cattle.
The concentration of acetate in blood plasma was measured by both a gas-liquid chromatographic method and an enzymatic method using acetyl-CoA synthetase. When acetate was measured enzymatically without previous protein precipitation, the apparent concentration was lower than when the concentration measured by either a gas-chromatographic method or by the enzymatic method after protein precipitation with perchloric acid and neutralization.
Björkman
C
C
Swedish University of Agricultural Sciences, Department of Cattle and Sheep Diseases, Uppsala.
D000106:Acetate-CoA Ligase; D000085:Acetates / Q000097:blood*; D019342:Acetic Acid; D000818:Animals; D001798:Blood Proteins / Q000737:chemistry; D002417:Cattle / Q000097:blood*; D011232:Chemical Precipitation; D002849:Chromatography, Gas; D004796:Clinical Enzyme Tests; D005260:Female
D000085:Acetates; D001798:Blood Proteins; D000106:Acetate-CoA Ligase; D019342:Acetic Acid