Generation of deletions in pTV1ts following transformation of Staphylococcus aureus protoplasts.
Plasmid pTV1ts was introduced into Staphylococcus aureus by transformation of protoplasts at frequencies ranging from 6.6 x 10(1) to 2.8 x 10(5) per micrograms of DNA when selection was made for resistance to chloramphenicol and erythromycin, respectively. Phenotypic analysis of regenerated transformants demonstrated three distinct classes. Analysis of the plasmid profiles of several isolates revealed that two classes harboured deleted pTV1ts derivatives.
Department of Food Microbiology & Toxicology, University of Wisconsin, Madison 53706.
J B
JB
Luchansky
N G
NG
Faith
D002701:Chloramphenicol / Q000494:pharmacology; D002872:Chromosome Deletion; D004269:DNA, Bacterial / Q000032:analysis; D004587:Electrophoresis, Agar Gel; D004917:Erythromycin / Q000494:pharmacology; D010641:Phenotype; D010957:Plasmids; D011523:Protoplasts; D015183:Restriction Mapping; D013211:Staphylococcus aureus / Q000187:drug effects / Q000235:genetics*; D014170:Transformation, Genetic
D004269:DNA, Bacterial; D004917:Erythromycin; D002701:Chloramphenicol