A practical test for in vitro evaluation of photosensitization: assessment with hematoporphyrin derivative (HPD).
A simple procedure is described for the determination of the photosensitizing potency of drugs, using three leukemic cell lines, two of lymphocytic origin, L1210 and P388 and one of erythroid type, Friend-745. The procedure allows one to investigate several aspects of the photosensitization properties of tested compounds such as cellular localization and direct (trypan blue exclusion) or delayed (clonogenicity) photomediated toxicities. The method was assessed using crude hematoporphyrin derivative (HPD) as well as dihematoporphyrin ether (DHE) or commercially available Photofrin II. Results were compared to those obtained with normal cells, e.g spleen lymphocytes and erythropoietic stem cells (CFU-e), and discussed in the light of the relative response of normal versus transformed cells.
INSERM U219, Institut Curie, Laboratoires 110-112 Centre Universitaire, Orsay, France.
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Croisy
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Carrez
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Zilberfarb
D000818:Animals; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D004354:Drug Screening Assays, Antitumor / Q000379:methods; D005260:Female; D017324:Hematoporphyrin Derivative; D006414:Hematoporphyrin Photoradiation; D006415:Hematoporphyrins / Q000378:metabolism / Q000494:pharmacology*; D007942:Leukemia, Experimental; D051379:Mice; D008811:Mice, Inbred DBA; D013154:Spleen / Q000378:metabolism; D013997:Time Factors; D014407:Tumor Cells, Cultured / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects*
D006415:Hematoporphyrins; D017324:Hematoporphyrin Derivative