Use of pulsed-field gel electrophoresis for investigation of hospital outbreaks of Acinetobacter baumannii.
Genomic DNAs from taxonomically and epidemiologically well-defined strains of Acinetobacter baumannii were digested with restriction endonucleases that cleave with low frequency, and the fragments were separated by pulse-field gel electrophoresis. Restriction fragment length polymorphisms were observed. Restriction fragment length polymorphism analysis can be used as an epidemiological tool to delineate outbreaks of nosocomial infections caused by A. baumannii.
Faculte de Medecine, Institut National de la Santé et de la Recherche Médicale, Unité U 65, Montpellier-Nîmes, France.
A
A
Gouby
M J
MJ
Carles-Nurit
N
N
Bouziges
G
G
Bourg
R
R
Mesnard
P J
PJ
Bouvet
D000150:Acinetobacter / Q000145:classification / Q000235:genetics*; D000151:Acinetobacter Infections / Q000382:microbiology*; D003428:Cross Infection / Q000382:microbiology*; D004269:DNA, Bacterial / Q000145:classification / Q000235:genetics*; D004196:Disease Outbreaks / Q000145:classification*; D016521:Electrophoresis, Gel, Pulsed-Field; D006801:Humans; D012150:Polymorphism, Restriction Fragment Length
D004269:DNA, Bacterial