Sulfoglycolipids bind to adhesive protein amphoterin (P30) in the nervous system.
HNK-1 antibody reactive carbohydrate epitope carried by glycolipids and glycoproteins has been shown to be involved in cell to cell interactions. It has been proposed that the HNK-1 reactive 3-sulfoglucuronyl carbohydrate epitope in glycolipids may interact with a cell surface receptor to promote the biological response in the developing nervous system. The possible occurrence of such a receptor was examined in rat nervous system. A specific binding of sulfoglycolipids to a 30 kD protein from adult rat cerebellum is described. Little binding was found with neutral glycolipids and gangliosides. The 30 kD protein from cerebellum was partially purified on a sulfatide-octyl-Sepharose affinity column. Binding of sulfoglucuronyl glycolipids to a similar 30 kD protein from forebrain previously identified as amphoterin is also shown. Amphoterin is developmentally regulated and is involved in neural cell adhesion and neurite extension.
Department of Biochemistry, Eunice Kennedy Shriver Center, Waltham, MA 02254.
P S
PS
Mohan
J
J
Laitinen
J
J
Merenmies
H
H
Rauvala
F B
FB
Jungalwala
D000818:Animals; D000943:Antigens, Differentiation / Q000276:immunology; D018959:CD57 Antigens; D002352:Carrier Proteins / Q000302:isolation & purification / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D002531:Cerebellum / Q000378:metabolism*; D002846:Chromatography, Affinity; D003600:Cytosol / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000032:analysis; D005260:Female; D006017:Glycolipids / Q000378:metabolism*; D024243:HMGB1 Protein; D006609:High Mobility Group Proteins / Q000302:isolation & purification / Q000378:metabolism*; D008715:Methionine / Q000378:metabolism; D008970:Molecular Weight; D009419:Nerve Tissue Proteins / Q000378:metabolism; D016548:Prosencephalon / Q000378:metabolism; D011485:Protein Binding; D051381:Rats; D011919:Rats, Inbred Strains; D014316:Tritium
D000943:Antigens, Differentiation; D018959:CD57 Antigens; D002352:Carrier Proteins; D000939:Epitopes; D006017:Glycolipids; D024243:HMGB1 Protein; D006609:High Mobility Group Proteins; D009419:Nerve Tissue Proteins; C011117:sulfoglycolipids; D014316:Tritium; D008715:Methionine