A novel synthetic method for hybridoma cell encapsulation.
We report here what we believe is the first example of the encapsulation of hybridoma cells within a synthetic polymer by a simple gelation with dissolved cations in water, and at room temperature. Two lines of hybridoma cells were encapsulated within calcium cross-linked polyphosphazene gel microbeads without affecting their viability or their capability to produce antibodies. Interaction of these gel beads with the positively-charged polyelectrolyte, poly(L-lysine), of 102-kD molecular weight, produced a semipermeable membrane that was capable of retaining the cell-secreted antibodies inside the beads. Cell density increased 3.5-fold within 13 days concomitant with a 6.4-fold increase in antibody production. These synthetic membranes have the potential to aid in protein recovery schemes.
Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge 02139.
M
M
Carmen Bañó
S
S
Cohen
K B
KB
Visscher
H R
HR
Allcock
R
R
Langer
D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D001709:Biotechnology / Q000379:methods*; D005782:Gels; D006825:Hybridomas; D008567:Membranes, Artificial; D009943:Organophosphorus Compounds / Q000138:chemical synthesis; D011107:Polylysine; D011108:Polymers / Q000138:chemical synthesis; D013696:Temperature; D014867:Water
D000911:Antibodies, Monoclonal; C070410:Ca-poly(bis(carboxylatophenoxy)phosphazene); D005782:Gels; D008567:Membranes, Artificial; D009943:Organophosphorus Compounds; D011108:Polymers; D014867:Water; D011107:Polylysine