Lack of expression of P-glycoprotein in 7 small cell lung cancer cell lines established both from untreated and from treated patients.
The establishment and characterisation of 7 small cell lung cancer cell lines is described. Four cell lines were established from biopsies taken from untreated patients and one of these was from primary tumour taken via the fibreoptic bronchoscope. The other three were from biopsies taken from patients who had relapsed after chemotherapy. All grow as non-adherent aggregates of cell and express a range of neuroendocrine and epithelial features characteristics of small cell lung cancer cell lines. No relationship was observed between the characteristics of the cell line in vitro and the treatment status of the patient from whom the biopsy was taken. Furthermore, none of the cell lines expressed p-glycoprotein even though 3 were derived from relapse biopsies where chemotherapy included drugs associated with the multidrug resistance phenotype.
CRC Department of Medical Oncology, University of Glasgow, U.K.
R
R
Milroy
J A
JA
Plumb
P
P
Batstone
A
A
Maclay
G C
GC
Wishart
F G
FG
Hay
W
W
Candlish
R
R
Adamson
M Z
MZ
Khan
S
S
Banham
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D000328:Adult; D000368:Aged; D018288:Carcinoma, Small Cell / Q000737:chemistry* / Q000235:genetics / Q000473:pathology; D002869:Chromosome Aberrations; D003402:Creatine Kinase / Q000032:analysis; D004296:Dopa Decarboxylase / Q000032:analysis; D004351:Drug Resistance; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008175:Lung Neoplasms / Q000737:chemistry* / Q000235:genetics / Q000473:pathology; D008297:Male; D008562:Membrane Glycoproteins / Q000032:analysis*; D008875:Middle Aged; D014407:Tumor Cells, Cultured
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D008562:Membrane Glycoproteins; D003402:Creatine Kinase; D004296:Dopa Decarboxylase