Identification and characterization of a partial cDNA expressing interleukin-1-like activity in keratinocytes.
A partial cDNA has been isolated from the human keratinocyte cell line COLO-16 which is distinct from either IL-1 alpha or beta and encodes a protein which displays some of the biological properties associated with IL-1. The 720 bp partial cDNA hybridized on Northern blots of COLO-16 mRNA to a 1.6 kbp message of low abundance. Expression of the partial cDNA in COS-1 cells resulted in activity in three IL-1 assays: thymocyte co-stimulation, D10.G4.1 T-cell stimulation and fibroblast proliferation. Antisera generated against synthetic peptides based on inferred protein sequence from the cDNA reacted with a 20 kDa and 30 kDa species in both the COLO-16 cell line and PMA-stimulated normal human keratinocytes. These novel species were also present in PMA-stimulated and unstimulated human dermal fibroblasts and human T-cell lines.
Department of Biochemistry, McMaster University Health Sciences Centre, Hamilton, Ontario, Canada.
T V
TV
Arsenault
D N
DN
Sauder
C B
CB
Harley
R C
RC
McKenzie
D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D015153:Blotting, Western; D002294:Carcinoma, Squamous Cell / Q000276:immunology; D002455:Cell Division / Q000276:immunology; D002460:Cell Line; D004247:DNA / Q000032:analysis; D015342:DNA Probes; D005347:Fibroblasts / Q000502:physiology; D006801:Humans; D007375:Interleukin-1 / Q000235:genetics*; D015603:Keratinocytes / Q000276:immunology*; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D012313:RNA / Q000032:analysis; D014162:Transfection
D015342:DNA Probes; D007375:Interleukin-1; D012313:RNA; D004247:DNA