[Measurement of the kinetics of cell proliferation of cancer of the oropharynx in vivo by the incorporation of bromodeoxyuridine and flow cytometry].
Forty-six tumors from patients with oropharyngeal carcinoma were analysed by flow cytometry after injection of bromodeoxyuridine (Budr) for the labelling index, the duration of S phase and the potential doubling time (Tpot). The results show large variations in Tpot (from 2.6 to 16.7 days) among these tumors from the same site and with the same histology. The variations in Tpot were not significantly related to TNM status and differentiation grade. However, aneuploid tumors had statistically significant shorter Tpot. The predictive value of Tpot regarding the response to radiotherapy is currently under investigation.
Département de radiothérapie, Institut Gustave-Roussy, villejuif, France.
J
J
Bourhis
G D
GD
Wilson
F
F
Janot
N
N
Chavaudra
A M
AM
Leridant
P
P
Wibault
T
T
Girinsky
B
B
Luboinski
F
F
Eschwege
E P
EP
Malaise
D001973:Bromodeoxyuridine; D002455:Cell Division; D004273:DNA, Neoplasm / Q000032:analysis; D005434:Flow Cytometry; D006801:Humans; D009367:Neoplasm Staging; D009959:Oropharyngeal Neoplasms / Q000473:pathology*; D016196:S Phase
D004273:DNA, Neoplasm; D001973:Bromodeoxyuridine
Mesure de la cinétique de prolifération in vivo des cancers de l'oropharynx par incorporation de bromodesoxyuridine et cytométrie de flux.