Induction of immediate-early gene proteins in dentate granule cells and somatostatin interneurons after hippocampal seizures.
The expression of the protein products of the immediate-early genes c-fos, Fos B, Fos-related proteins (FRAs), c-jun, jun B, jun D and krox-24 was investigated in the rat hippocampus at various times after electrically-induced hippocampal seizures. Hippocampal seizures induced all the immediate-early gene proteins in dentate granule cells with differing time-courses. In addition, Krox-24, Fos and Jun D were also induced in somatostatin-containing interneurons throughout the hippocampus and also in a small percentage of parvalbumin-containing interneurons. Thus, hippocampal seizures induce waves of immediate-early gene protein expression in dentate granule cells and a selective expression of krox-24, Fos and Jun D in hippocampal somatostatin interneurons. These results suggest that biochemical and/or morphological changes occurring in dentate granule cells and somatostatin interneurons after seizures may be regulated by immediate-early gene expression, and that these immediate-early gene proteins may be involved in seizure development in the nervous system.
Dragunow
M
M
Department of Pharmacology, School of Medicine, University of Auckland, New Zealand.
Yamada
N
N
Bilkey
D K
DK
Lawlor
P
P
D000818:Animals; D004268:DNA-Binding Proteins / Q000032:analysis / Q000235:genetics*; D051766:Early Growth Response Protein 1; D004558:Electric Stimulation; D015870:Gene Expression; D016762:Genes, fos; D016758:Genes, jun; D006624:Hippocampus / Q000502:physiology / Q000503:physiopathology*; D017874:Immediate-Early Proteins; D007150:Immunohistochemistry; D007395:Interneurons / Q000502:physiology*; D007700:Kinetics; D008297:Male; D016760:Proto-Oncogene Proteins c-fos / Q000032:analysis / Q000235:genetics*; D016755:Proto-Oncogene Proteins c-jun / Q000032:analysis / Q000235:genetics*; D051381:Rats; D011919:Rats, Inbred Strains; D012016:Reference Values; D012640:Seizures / Q000503:physiopathology*; D013004:Somatostatin / Q000502:physiology*; D013997:Time Factors; D014157:Transcription Factors / Q000032:analysis / Q000235:genetics*; D016335:Zinc Fingers / Q000235:genetics
D004268:DNA-Binding Proteins; D051766:Early Growth Response Protein 1; C486110:Egr1 protein, rat; D017874:Immediate-Early Proteins; D016760:Proto-Oncogene Proteins c-fos; D016755:Proto-Oncogene Proteins c-jun; D014157:Transcription Factors; D013004:Somatostatin