Oxygen radical-mediated mutagenic effect of asbestos on human lymphocytes: suppression by oxygen radical scavengers.
The mutagenic effect of chrysotile asbestos fibers and zeolite and latex particles on human lymphocytes in whole blood has been studied. It was concluded that their mutagenic activities were mediated by oxygen radicals because they were inhibited by antioxidant enzymes (SOD and catalase) and oxygen radical scavengers (rutin, ascorbic acid, and bemitil). It was proposed that oxygen radicals were released by phagocytes activated upon exposure to mineral dusts and fibers. The study of lucigenin- and luminol-amplified chemiluminescence of peritoneal macrophages stimulated by chrysotile fibers and zeolite and latex particles has shown that their mutagenic action is probably mediated by different oxygen species, namely, by the iron-oxygen complexes (perferryl ions) plus hydrogen peroxide, hydrogen peroxide, and superoxide ion, respectively. From the oxygen radical scavengers studied, rutin was the most effective inhibitor of the mutagenic effect of mineral fibers and dusts.
Korkina
L G
LG
2nd Moscow Medical Institute, U.S.S.R.
Durnev
A D
AD
Suslova
T B
TB
Cheremisina
Z P
ZP
Daugel-Dauge
N O
NO
Afanas'ev
I B
IB
D000818:Animals; D001194:Asbestos / Q000037:antagonists & inhibitors / Q000633:toxicity*; D001205:Ascorbic Acid / Q000494:pharmacology; D001562:Benzimidazoles / Q000494:pharmacology; D002374:Catalase / Q000378:metabolism; D002869:Chromosome Aberrations; D004391:Dust; D005609:Free Radicals; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D008163:Luminescent Measurements; D008214:Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D008903:Minerals; D009153:Mutagens / Q000378:metabolism* / Q000633:toxicity; D010100:Oxygen / Q000494:pharmacology*; D010586:Phagocytes / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D012431:Rutin / Q000494:pharmacology; D013482:Superoxide Dismutase / Q000378:metabolism
D001562:Benzimidazoles; D004391:Dust; D005609:Free Radicals; D008903:Minerals; D009153:Mutagens; D001194:Asbestos; D012431:Rutin; C049825:bemethyl; D006861:Hydrogen Peroxide; D002374:Catalase; D013482:Superoxide Dismutase; D001205:Ascorbic Acid; D010100:Oxygen