HPV-16-related DNA sequences in Kaposi's sarcoma.
In the USA, Kaposi's sarcoma associated with the acquired immunodeficiency syndrome (AIDS-KS) is ten times more common in homosexual or bisexual men than in heterosexual men with AIDS. One explanation for this finding is that AIDS-KS may be caused by an infectious agent. Because there is a high incidence of human papillomavirus (HPV) infection, especially HPV-16, in homosexual men, we have sought HPV DNA sequences in Kaposi's sarcoma. We used the polymerase chain reaction with a primer pair specific for the highly conserved E6 region of HPV-16 to detect HPV-16 homologous DNA fragments in tumour tissues from 97 patients with KS and in KS-derived cell cultures. HPV DNA sequences were found in 11 of 69 KS skin tumours from homosexual men with AIDS-KS, in 3 of 11 KS biopsy specimens from homosexual men who had no clinical or laboratory evidence of HIV-infection, and in 5 of 17 KS skin lesions from HIV-1-negative elderly men and women with classic KS. The same primer pair amplified HPV-16 homologous fragments from two different continuous cell cultures derived from pleural effusion fluid of patients with pulmonary AIDS-KS and two continuous cell cultures derived from KS skin lesions. The findings suggest that HPV-16-related DNA sequences are associated with different forms of KS and may have a role in the pathogenesis of this neoplasm.
Huang
Y Q
YQ
Department of Microbiology, New York University School of Medicine, New York.
Li
J J
JJ
Rush
M G
MG
Poiesz
B J
BJ
Nicolaides
A
A
Jacobson
M
M
Zhang
W G
WG
Coutavas
E
E
Abbott
M A
MA
Friedman-Kien
A E
AE
D000163:Acquired Immunodeficiency Syndrome / Q000150:complications; D015139:Blotting, Southern; D015344:DNA Probes, HPV; D004279:DNA, Viral / Q000032:analysis* / Q000235:genetics; D005260:Female; D006801:Humans; D008297:Male; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D012514:Sarcoma, Kaposi / Q000209:etiology / Q000382:microbiology*; D012878:Skin Neoplasms / Q000209:etiology / Q000382:microbiology*; D014407:Tumor Cells, Cultured / Q000382:microbiology
D015344:DNA Probes, HPV; D004279:DNA, Viral