Sheep linkage mapping: restriction fragment length polymorphism detection with heterologous cDNA probes.
A selection of cattle, human and sheep cDNA probes were screened against sheep genomic DNA, cut with 10 different restriction enzymes, to assess the usefulness of these probes for restriction fragment length polymorphism (RFLP) linkage studies in sheep. Two-thirds of the cattle cDNA probes showed moderate to strong homology with sheep DNA samples, compared with less than half of the human cDNA probes at the final washing stringency chosen for the experiments. The set of probes tested detected a useful frequency of RFLPs. Fifty-seven per cent of probes showing moderate to strong homology identified RFLPs with one or more restriction enzymes. Restriction enzymes that detected RFLPs most frequently in sheep were TaqI and MspI. The results show that sheep and cattle cDNA probes, including candidate genes for production traits, identified a high frequency of RFLPs suitable for genetic mapping in sheep.
Department of Biochemistry, University of Otago, Dunedin, New Zealand.
G W
GW
Montgomery
J A
JA
Sise
J M
JM
Penty
H M
HM
Tou
D F
DF
Hill
D000818:Animals; D002417:Cattle; D015342:DNA Probes; D004262:DNA Restriction Enzymes / Q000378:metabolism; D003670:Deer; D005069:Evaluation Studies as Topic; D008040:Genetic Linkage; D006041:Goats; D006801:Humans; D012150:Polymorphism, Restriction Fragment Length; D012756:Sheep / Q000235:genetics*; D013045:Species Specificity
D015342:DNA Probes; D004262:DNA Restriction Enzymes