Localization of the nucleotide excision repair gene ERCC6 to human chromosome 10q11-q21.
We have cloned the human DNA excision repair gene ERCC6 by virtue of its ability to correct the uv sensitivity of Chinese hamster overy cell mutant UV61. This mutant is a member of complementation group 6 of the nucleotide excision repair-deficient rodent mutants. By means of in situ hybridization and Southern blot analysis of mouse x human somatic cell hybrids, the gene was localized to human chromosome 10q11-q21. An RFLP detected within the ERCC6 locus can be helpful in linkage analysis.
Troelstra
C
C
Department of Cell Biology and Genetics, Erasmus University Rotterdam, The Netherlands.
Landsvater
R M
RM
Wiegant
J
J
van der Ploeg
M
M
Viel
G
G
Buys
C H
CH
Hoeijmakers
J H
JH
D000818:Animals; D016466:CHO Cells; D002874:Chromosome Mapping; D002879:Chromosomes, Human, Pair 10; D006224:Cricetinae; D004260:DNA Repair / Q000235:genetics* / Q000528:radiation effects; D005260:Female; D008040:Genetic Linkage; D006801:Humans; D006822:Hybrid Cells; D008297:Male; D009154:Mutation; D010375:Pedigree; D012150:Polymorphism, Restriction Fragment Length; D015183:Restriction Mapping; D014466:Ultraviolet Rays