"Primatization" of recombinant antibodies for immunotherapy of human diseases: a macaque/human chimeric antibody against human CD4.
Immunoglobulin variable region genes from non-human primates, cynomolgus macaques, were shown to have 85%-98% homology with human immunoglobulin sequences and yet macaques are phylogenetically distant enough to respond against conserved human antigens. Immunoglobulin genes were isolated from monkeys immunized with human CD4 antigen and a human/monkey chimeric anti-CD4 antibody with 91-92% homology to human immunoglobulin framework regions was cloned and expressed. The antibody has an apparent affinity of 3.2 x 10(-11) M and exhibits potent immunosuppressive properties in vitro.
IDEC Pharmaceuticals Corporation, La Jolla, CA 92037.
R
R
Newman
J
J
Alberts
D
D
Anderson
K
K
Carner
C
C
Heard
F
F
Norton
R
R
Raab
M
M
Reff
S
S
Shuey
N
N
Hanna
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000627:therapeutic use*; D001483:Base Sequence; D015704:CD4 Antigens / Q000276:immunology*; D003001:Cloning, Molecular; D006801:Humans; D007136:Immunoglobulins / Q000235:genetics; D007167:Immunotherapy / Q000379:methods*; D008252:Macaca fascicularis; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000032:analysis / Q000627:therapeutic use; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D013601:T-Lymphocytes / Q000276:immunology
D000911:Antibodies, Monoclonal; D015704:CD4 Antigens; D007136:Immunoglobulins; D011994:Recombinant Proteins