Analysis of T lymphocyte subsets proliferating in response to infective and UV-inactivated African swine fever viruses.
The proliferative response to infective and UV-inactivated African swine fever virus was analyzed in cells from pigs surviving an experimental infection with attenuated virus. All the pigs showed strong dose-dependent proliferative responses to both infective and UV-inactivated virus. This response was also observed when nitrocellulose-bound solubilized virus proteins were used in the assay. Heterologous isolates also induced proliferation, however it was significantly lower than that induced by the isolate used to infect the animals. The response to infective virus was blocked equally by anti-CD4 and anti-CD8 monoclonal antibodies (mAb); the response to UV-inactivated virus was almost abolished by anti-CD4 and 60% inhibited by anti-CD8 mAb. FACS analysis of 28-day T cell lines derived from peripheral blood mononuclear cells demonstrated the progressive increase of the CD8+ subset when the cells were stimulated with infective virus, whereas the stimulation with UV-inactivated virus induced the increase of both CD4+ and CD8+ subsets. In this case, the sum of CD4+ and CD8+ percentages was higher than the total percentage of T cells, suggesting the presence of cells positive for both CD4+ and CD8+.
Departamento de Sanidad Animal, INIA, Madrid, Spain.
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A
Canals
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F
Alonso
J
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Tomillo
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J
Domínguez
D000357:African Swine Fever / Q000276:immunology*; D000358:African Swine Fever Virus / Q000276:immunology* / Q000528:radiation effects; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D015704:CD4 Antigens / Q000276:immunology; D016516:CD4-CD8 Ratio; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D016827:CD8 Antigens / Q000276:immunology; D002460:Cell Line; D002469:Cell Separation; D005434:Flow Cytometry; D007700:Kinetics; D008213:Lymphocyte Activation; D013552:Swine; D013556:Swine, Miniature; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*; D014466:Ultraviolet Rays
D000911:Antibodies, Monoclonal; D015704:CD4 Antigens; D016827:CD8 Antigens