New molecular biology methods for protein engineering.
This review outlines recent advances in the application of molecular biological techniques to the study of protein structure and function. The chapter is divided into four main sections: methods for oligonucleotide-directed mutagenesis; mutational strategies for identifying functional residues and domains; systems for expression; and future developments. Few new methods were reported in 1990; however, a number of the papers that appeared represent refinements of previously reported strategies. This review is also published in Current Opinion in Structural Biology 1991, 1:605-610.
Department of Protein Engineering, Genentech, South San Francisco, California 94080.
M J
MJ
Zoller
D001709:Biotechnology / Q000379:methods*; D003001:Cloning, Molecular; D016296:Mutagenesis; D015202:Protein Engineering / Q000379:methods*