Identification and quantification of rodent malaria strains and species using gene probes.
A DNA probe PCsv4 and a subclone thereof PCsv4.1, hybridize specifically to rodent malaria DNA. DNA purified from a small volume (10 microliters) of infected mouse blood was used to determine the composition of the parasite population present. The hybridization signal following PCsv4 probing of slot-blotted DNA correlated directly with parasitaemia. The hybridization pattern and intensity, resulting from probing restriction enzyme digested and Southern-blotted genomic DNA, determined the identity of the infecting parasite line(s), and provided a semi-quantitative measure of parasite burden. Fifteen parasite lines representative of all four Plasmodium species infecting rodents can be differentiated in this way.
Division of Parasitology, National Institute for Medical Research, Mill Hill, London.
G
G
Snounou
T
T
Bourne
W
W
Jarra
S
S
Viriyakosol
K N
KN
Brown
D000818:Animals; D001345:Autoradiography; D015139:Blotting, Southern; D015342:DNA Probes; D016054:DNA, Protozoan / Q000032:analysis*; D015246:Deoxyribonuclease EcoRI; D004195:Disease Models, Animal; D008288:Malaria / Q000469:parasitology*; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D009693:Nucleic Acid Hybridization; D010961:Plasmodium / Q000145:classification* / Q000235:genetics; D010962:Plasmodium berghei / Q000145:classification / Q000235:genetics; D016788:Plasmodium chabaudi / Q000145:classification / Q000235:genetics; D010967:Plasmodium yoelii / Q000145:classification / Q000235:genetics; D012150:Polymorphism, Restriction Fragment Length; D012044:Regression Analysis; D015183:Restriction Mapping
D015342:DNA Probes; D016054:DNA, Protozoan; D015246:Deoxyribonuclease EcoRI