Glutamate-induced ribosomal disaggregation and ultrastructural changes in rat cortical neuronal culture: protective effect of horse serum.
Differentiated primary cortical neuronal cultures of rat were exposed for 5 min to 0.1 and 1.0 mM glutamate. In cultures maintained in serum-free medium after glutamate exposure, ribosomes completely disaggregated and neurons died within 24 h already after 0.1 mM glutamate. Addition of 5% horse serum to the culture medium prevented both ribosomal disaggregation and neuronal death even after exposure to 1.0 mM glutamate. Glutamate toxicity in vitro requires removal of serum-associated growth factors from the incubation medium and, therefore, may not be representative for neuronal vulnerability in vivo.
Max-Planck-Institute for Neurological Research, Department for Experimental Neurology, Cologne, FRG.
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Oschlies
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Wiessner
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Hossmann
D000818:Animals; D001790:Blood Physiological Phenomena; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology*; D005971:Glutamates / Q000494:pharmacology*; D018698:Glutamic Acid; D006736:Horses; D009474:Neurons / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D051381:Rats; D017208:Rats, Wistar; D012270:Ribosomes / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure
D005971:Glutamates; D018698:Glutamic Acid