IL-4 decreases IFN-gamma-induced endothelial ICAM-1 expression by a transcriptional mechanism.
Intercellular adhesion molecule-1 (ICAM-1, CD54) is a cell surface ligand for LFA-1 (CD11a/18). Several cytokines, such as IL-1, TNF-alpha or IFN-gamma, have been shown to increase the ICAM-1 expression on cultured endothelial cells. Here we show that IL-4 decreases the IFN-gamma-induced ICAM-1 expression on an endothelial cell line (EA.hy 926), whereas IL-4 alone has practically no effects on ICAM-1 expression. The down-regulative effect was also seen at the mRNA level suggesting that this regulation process is transcriptional. At the same time IL-4 was unable to alter the TNF-alpha-induced ICAM-1 up-regulation. Both IFN-gamma and TNF-alpha are able to decrease the proliferation of the endothelial cell line, but IL-4 could not modify this response when added together with the other cytokines. Taken together we show in this paper that IL-4 decreases IFN-gamma but not TNF-alpha-induced up-regulation of ICAM-1 expression on EA.hy 926 cells.
Renkonen
R
R
Department of Bacteriology and Immunology, University of Helsinki, Finland.
Mattila
P
P
Majuri
M L
ML
Paavonen
T
T
Silvennoinen
O
O
D015152:Blotting, Northern; D015815:Cell Adhesion Molecules / Q000096:biosynthesis*; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D015536:Down-Regulation; D004347:Drug Interactions; D004727:Endothelium / Q000378:metabolism* / Q000502:physiology; D005434:Flow Cytometry; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D018799:Intercellular Adhesion Molecule-1; D007371:Interferon-gamma; D015847:Interleukin-4 / Q000494:pharmacology*; D012333:RNA, Messenger / Q000032:analysis; D014158:Transcription, Genetic / Q000187:drug effects*; D014409:Tumor Necrosis Factor-alpha
D015815:Cell Adhesion Molecules; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D018799:Intercellular Adhesion Molecule-1; D015847:Interleukin-4; D007371:Interferon-gamma