Extraction of penicillin G from simulated media by an emulsion liquid membrane process.
The extraction of penicillin G from simulated media was performed by water/oil/water (w/o/w) emulsion liquid membranes (ELMs) and studied under various operational conditions in a batch system. The degree of extraction achieved was between 80% and 95% under specific conditions. A concentration of greater than nine times the initial concentration of penicillin G in the external phase was obtained in the internal phase. The pH of the internal aqueous solution, containing a basic salt, was theoretically calculated on the basis of the amount of penicillin G transported into the internal phase. The calculated results agreed with the experimental data well and were used to select a suitable type and concentration of a basic salt in the internal phase to give a pH within the range 5 to 8 where penicillin G was stable after the termination of extraction. The extraction of penicillin G was successfully performed by the ELM process with sodium carbonate in the internal phase.
Department of Chemical Engineering, Korea Advanced Institute of Science and Technology, Taejon.
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Lee
D001709:Biotechnology; D003470:Culture Media; D004355:Drug Stability; D004655:Emulsions; D006863:Hydrogen-Ion Concentration; D010400:Penicillin G / Q000302:isolation & purification*; D012996:Solutions; D013501:Surface-Active Agents; D014867:Water
D003470:Culture Media; D004655:Emulsions; D012996:Solutions; D013501:Surface-Active Agents; D014867:Water; D010400:Penicillin G