Intestinal acid inhibits gastric acid secretion by neural and hormonal mechanisms in rats.
To determine the relative contributions of neural reflexes and intestinal hormones to the inhibition of gastric acid secretion by intestinal acidification, rats with an extrinsically denervated, transplanted segment of jejunum, and those with an innervated segment of jejunum, were studied. Postoperatively, meal-stimulated gastric acid secretion was measured. When the acid secretory response to intragastric liver extract reached a plateau, graded concentrations of hydrochloric acid or saline were instilled into the jejunal segments. Gastric acid secretion was inhibited by intrajejunal acid (pH 2.5) by 79% in the innervated rats and by 64% in the transplanted group. Thus at a pH of 2.5 there was a 15% greater maximum inhibition of plateau acid response in the innervated rats than in the transplanted rats, presumably because of the extrinsic neural contribution. To examine the hormonal mediators, the effects of a somatostatin monoclonal antibody and a CCK-A receptor antagonist (L 364718) on acid-induced inhibition of gastric acid secretion were studied in transplanted rats. Treatment with a somatostatin monoclonal antibody or with L 364718 reduced the acid-induced (pH 2.5) inhibition of gastric acid secretion by 93 and 27%, respectively. Jejunal acidification inhibits gastric acid secretion in the rat by both neural and hormonal mechanisms. The hormonal mechanism is mediated by somatostatin and CCK.
Department of Physiology, University of California, San Francisco 94143.
S L
SL
Orloff
N W
NW
Bunnett
J H
JH
Walsh
H T
HT
Debas
D000143:Acids / Q000378:metabolism*; D000818:Animals; D000911:Antibodies, Monoclonal; D001570:Benzodiazepinones / Q000494:pharmacology; D002766:Cholecystokinin / Q000037:antagonists & inhibitors / Q000502:physiology*; D020109:Devazepide; D004435:Eating; D005744:Gastric Acid / Q000378:metabolism*; D006851:Hydrochloric Acid / Q000494:pharmacology; D007413:Intestinal Mucosa / Q000378:metabolism*; D007422:Intestines / Q000294:innervation; D007583:Jejunum / Q000378:metabolism / Q000637:transplantation; D008297:Male; D009424:Nervous System Physiological Phenomena; D051381:Rats; D011917:Rats, Inbred Lew; D012965:Sodium Chloride / Q000494:pharmacology; D013004:Somatostatin / Q000502:physiology*
D000143:Acids; D000911:Antibodies, Monoclonal; D001570:Benzodiazepinones; D012965:Sodium Chloride; D013004:Somatostatin; D002766:Cholecystokinin; D020109:Devazepide; D006851:Hydrochloric Acid