Heat shock proteins of barley mitochondria and chloroplasts. Identification of organellar hsp 10 and 12: putative chaperonin 10 homologues.
Tissue slices from barley seedlings were subjected to heat shock and metabolically labelled with [35S]methionine and [35S]cysteine. Mitochondria and chloroplasts were isolated and shown to contain two novel heat shock proteins of 10 and 12 kDa, respectively. The possibility that these proteins, like a mitochondrial 10 kDa stress protein recently isolated from rat hepatoma cells [(1992) Proc. Natl. Acad. Sci. 89, in press] represent eukaryotic chaperonin 10 homologues is discussed.
Department of Biochemistry, La Trobe University, Bundoora, Victoria, Australia.
D J
DJ
Hartman
D
D
Dougan
N J
NJ
Hoogenraad
P B
PB
Høj
D018833:Chaperonins; D002736:Chloroplasts / Q000737:chemistry*; D004586:Electrophoresis; D006360:Heat-Shock Proteins / Q000032:analysis*; D001467:Hordeum / Q000737:chemistry*; D008928:Mitochondria / Q000737:chemistry*; D010940:Plant Proteins / Q000032:analysis*; D011506:Proteins / Q000032:analysis
D006360:Heat-Shock Proteins; D010940:Plant Proteins; D011506:Proteins; D018833:Chaperonins