Design of two immobilized cell catalysts by entrapment on gelatin: internal diffusion aspects.
Experimental results obtained during the design of two immobilized cell catalysts by entrapment on gelatin are presented. Strong diffusional limitations are found and explained with the usual parameters and models, introducing an empirical correlation between substrate concentration and effectiveness factor. The effect of particle size, enzyme load, and specific activity in the system is discussed in terms of cooperation between bioengineers and geneticists.
Centro de Investigación sobre Ingeniería Genética y Biotecnología, UNAM, Cuernavaca, Morelos, Mexico.
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E
Castillo
M
M
Rodríguez
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L
Casas
R
R
Quintero
A
A
López-Munguía
D002384:Catalysis; D004800:Enzymes, Immobilized / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology*; D005780:Gelatin; D007700:Kinetics; D007716:Kluyveromyces / Q000201:enzymology*; D010399:Penicillin Amidase / Q000378:metabolism*; D001616:beta-Galactosidase / Q000378:metabolism*
D004800:Enzymes, Immobilized; D005780:Gelatin; D001616:beta-Galactosidase; D010399:Penicillin Amidase