A new cDNA clone relating to larger molecular species of rat insulin-like growth factor-I mRNA.
A new cDNA clone for rat IGF-I mRNA was obtained. The cDNA contained a new base sequence as cDNA in the 3' region. The sequence coincided with a part of the sequence reported earlier by Shimatsu and Rotwein in exon 5 of the rat IGF-I gene. The presence of this cDNA proved the previous suggestion to be true that the size heterogeneity of IGF-I mRNA is primarily due to the heterogeneity of the 3'-untranslated region.
Department of Agricultural Chemistry, Faculty of Agriculture, University of Tokyo, Japan.
H
H
Kato
A
A
Okoshi
Y
Y
Miura
T
T
Noguchi
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D005091:Exons; D007334:Insulin-Like Growth Factor I / Q000235:genetics*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000235:genetics*; D051381:Rats
D012333:RNA, Messenger; D007334:Insulin-Like Growth Factor I; D004247:DNA