Evaluation of very rapid emit Qst methods for measuring serum procainamide and N-acetylprocainamide concentrations.
This study assessed the Emit Qst procainamide (PA) and N-acetylprocainamide (NAPA) assays. Accuracy and intraday precision were evaluated by repeatedly measuring PA and NAPA concentrations in spiked serum samples using Qst and high-performance liquid chromatography methods. Interday precision was evaluated by measuring concentrations in spiked samples over 4 weeks. Correlation between methods was assessed in patient samples, and proportional, constant, and random errors were estimated. Intraday coefficients of variation (CVs) were below 6.4% for PA and NAPA for both methods; interday CVs were below 7.8%. The proportional, constant, and random errors of the PA Qst assay in patient samples were 5.7%, -0.224 mg/L, and +/- 0.574 mg/L, respectively. The same errors in the NAPA Qst assay were 17.2%, 0.229 mg/L, and +/- 1.79 mg/L, respectively. The Qst assays are rapid, accurate, and precise methods for routine clinical measurement of PA and NAPA, although the proportional error in the NAPA assay should be recognized.
College of Pharmacy, Ohio State University, Columbus 43210.
C A
CA
Carnes
J D
JD
Coyle
D000120:Acecainide / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D005069:Evaluation Studies as Topic; D006801:Humans; D007124:Immunoenzyme Techniques; D011342:Procainamide / Q000097:blood*; D013997:Time Factors
D000120:Acecainide; D011342:Procainamide