Cloning, mapping, and sequencing of 3' and its flanking region of bovine alpha-s1 casein gene.
A positive clone was isolated from a bovine genomic library in EMBL3 with the probe of alpha-s1 casein gene cDNA. The results from both restriction endonuclease digestion and Southern hybridization probed with the upstream and downstream fragments from the termination codon of bovine alpha-s1 casein gene cDNA suggested that the clone contained 3.5 kb downstream and 10.2 kb upstream fragments from the termination codon of alpha-s1 casein gene. Some divergences in restriction endonucleases sites, a few deletions, and many point mutations were found in introns and exons when compared with those which were published.
Chen
R
R
Institute of Biophysics, CAS, Beijing, China.
Wang
B
B
Zhang
Y
Y
Liu
W
W
Zhang
J
J
Lao
W
W
D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002364:Caseins / Q000235:genetics*; D002417:Cattle; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D003062:Codon; D018076:DNA, Complementary; D005091:Exons; D007438:Introns; D008969:Molecular Sequence Data; D017354:Point Mutation; D015183:Restriction Mapping
D002364:Caseins; D003062:Codon; D018076:DNA, Complementary