Southern blot hybridization analysis of polyoma virus-specific RNA synthesized under the block of virus replication by 5-bromo-2'-deoxyuridine.
Polyoma (Py) virus-specific RNA, synthesized at reduced level in infected cells in the presence of antiviral substance 5-bromo-2'-deoxyuridine was characterized in more detail by Southern blot hybridization analysis. Virus-specific RNA present in 3H-uridine labelled cytoplasmic preparations hybridized to individual viral DNA restriction fragments in a characteristic manner and extent, which was quantitatively assayed. BrdUrd (6.34 micrograms/ml) lowers the hybridization profiles essentially in a proportional manner, which indicates that no new, atypical transcription products are formed in the presence of BrdUrd.
Zemla
J
J
Institute of Virology, Slovak Academy of Sciences, Brałislava, Czechoslovakia.
Tarábek
J
J
D000818:Animals; D015139:Blotting, Southern; D001973:Bromodeoxyuridine / Q000494:pharmacology; D004279:DNA, Viral / Q000235:genetics; D051379:Mice; D009693:Nucleic Acid Hybridization; D011120:Polyomavirus / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D012367:RNA, Viral / Q000096:biosynthesis* / Q000302:isolation & purification; D014158:Transcription, Genetic / Q000187:drug effects; D014779:Virus Replication / Q000187:drug effects
D004279:DNA, Viral; D012367:RNA, Viral; D001973:Bromodeoxyuridine