Highly polymorphic region of the human prothrombin (F2) gene.
We have found a highly polymorphic region in the human prothrombin gene. Our sequence differed from that previously reported at as many as 6 positions in a 225-bp stretch spanning exon 6 and its flanking regions; four of these positions were related to endonuclease restriction sites for AluI, HpaII(MspI), MboII, and NcoI. AluI and HpaII digested all alleles of the Japanese tested. MboII and NcoI restriction fragment length polymorphisms are highly heterozygous and not in linkage disequilibrium; they thus serve as good human DNA markers.
Iwahana
H
H
Otsuka Department of Clinical and Molecular Nutrition, School of Medicine, University of Tokushima, Japan.
Yoshimoto
K
K
Itakura
M
M
D000595:Amino Acid Sequence; D001483:Base Sequence; D004706:Endodeoxyribonucleases / Q000378:metabolism; D005819:Genetic Markers / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D012150:Polymorphism, Restriction Fragment Length; D011516:Prothrombin / Q000235:genetics*
D005819:Genetic Markers; D011516:Prothrombin; D004706:Endodeoxyribonucleases