Genus- and species-specific oligonucleotide probes derived from 16S rRNA for the identification of vagococci.
Synthetic oligonucleotide probes specific for vagococci were designed from 16S rRNA sequence data. Molecular hybridizations with PCR-amplified rDNA targets provided an unequivocal means of differentiating vagococci from related lactic acid bacteria (eg. Carnobacterium, Enterococcus, Lactococcus) and identification at the generic and species levels.
Williams
A M
AM
Department of Microbiology, AFRC Institute of Food Research, Shinfield, Reading, UK.
Collins
M D
MD
D001483:Base Sequence; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D017045:Lactococcus / Q000235:genetics / Q000302:isolation & purification*; D013294:Lactococcus lactis / Q000235:genetics / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes / Q000737:chemistry*; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000737:chemistry*; D013045:Species Specificity; D013289:Streptococcaceae / Q000235:genetics / Q000302:isolation & purification*; D013291:Streptococcus / Q000235:genetics / Q000302:isolation & purification
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D015345:Oligonucleotide Probes; D012336:RNA, Ribosomal, 16S