Effect of carbachol on luminal release of somatostatin from isolated perfused rat duodenum.
The dynamic release of somatostatin-like immunoreactivity (SLI) from duodenum into the lumen was studied in the isolated, vascularly perfused rat duodenum. The luminal release of duodenal SLI was stimulated by a cholinergic agonist, carbachol, and the carbachol-induced release of SLI was completely blocked by atropine, but not by hexamethonium. These data suggest that the luminal release of SLI from rat duodenum is under the control of a cholinergic muscarinic stimulation. The ratio of somatostatin-14 to somatostatin-28 in picograms was about 1 during basal release but increased to approximately 2 during carbachol stimulation.
Department of Anatomy, Shiga University of Medical Science, Japan.
M
M
Fujimiya
C H
CH
McIntosh
H
H
Kimura
Y N
YN
Kwok
D000818:Animals; D001285:Atropine / Q000494:pharmacology; D002217:Carbachol / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D004386:Duodenum / Q000187:drug effects / Q000378:metabolism*; D006584:Hexamethonium Compounds / Q000494:pharmacology; D066298:In Vitro Techniques; D008297:Male; D010477:Perfusion; D051381:Rats; D017208:Rats, Wistar; D013004:Somatostatin / Q000378:metabolism*; D053561:Somatostatin-28
D006584:Hexamethonium Compounds; D013004:Somatostatin; D053561:Somatostatin-28; D001285:Atropine; D002217:Carbachol