Stearoyl-CoA desaturase gene expression in lymphocytes.
B lymphocytes from the spleens of normal (BALB/c) and autoimmune (MRL/lpr) strains of mice express the SCD-2 form of stearoyl-CoA desaturase as opposed to the SCD-1 form of the gene which is expressed in liver. However, whereas BALB/c T cells did not express SCD-1 or SCD-2, both BALB/c thymocytes and MRL/lpr T cells expressed SCD-2, suggesting a developmental down-regulation of SCD-2 within the T cell lineage. Northern analyses also revealed the expression of SCD-2 in the T cell lines BW5147, CTLL-2 and HT-2 and in BCL1, a B cell line. SCD-1 expression was not detected in any of the lymphoid cells tested. Finally, we show that SCD-2 gene expression is inhibited by arachidonic acid (20:4). These results demonstrate the complexity of SCD-2 regulation in lymphoid cells.
Tebbey
P W
PW
Dept. of Microbiology and Immunology, East Carolina University School of Medicine, Greenville, North Carolina 27858-4354.
Buttke
T M
TM
D000818:Animals; D001327:Autoimmune Diseases / Q000201:enzymology / Q000235:genetics; D015152:Blotting, Northern; D015704:CD4 Antigens / Q000032:analysis; D016827:CD8 Antigens / Q000032:analysis; D005260:Female; D005434:Flow Cytometry; D015870:Gene Expression; D015971:Gene Expression Regulation, Enzymologic; D008214:Lymphocytes / Q000201:enzymology* / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008817:Mice, Mutant Strains; D012333:RNA, Messenger / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D013154:Spleen / Q000201:enzymology; D013230:Stearoyl-CoA Desaturase / Q000235:genetics*; D013601:T-Lymphocytes / Q000201:enzymology* / Q000276:immunology
D015704:CD4 Antigens; D016827:CD8 Antigens; D012333:RNA, Messenger; D013230:Stearoyl-CoA Desaturase