[Characterization of cloned alpha-satellite sequence from the extrachromosomal DNA of HeLa cell culture].
We have obtained the alphoid DNA clones, pK1 and pK2, from the extrachromosomal DNA of Hela cells treated by cycloheximide (30 micrograms/ml). Nucleotide sequences of the clones were aligned. The sides of the pK1 and pK2 are 390 and 184 bp, respectively. The marked RELP for the clones was not observed. The results of in situ hybridization have shown an approximately equal distribution of Ag-grains over major part of human chromosomes, with a slight preference for chromosomes 1, 5 and 19 (the 1-st group of alpha-satellite DNA). Therefore, the obtained alphoid sequences seem to be rather conservative and non-chromosome-specific. We suppose that increase of the alphoid DNA content in the fraction of the extrachromosomal DNA under the cycloheximide treatment is a result of the sporadic statistical processes rather then consequence of the specific excision.
Krokhina
T B
TB
Tsvetkova
T G
TG
Gar'kavtsev
I V
IV
D001483:Base Sequence; D002878:Chromosomes, Human, Pair 1; D002888:Chromosomes, Human, Pair 19; D002895:Chromosomes, Human, Pair 5; D003001:Cloning, Molecular; D003513:Cycloheximide / Q000494:pharmacology; D004270:DNA, Circular / Q000235:genetics; D004276:DNA, Satellite; D005111:Extrachromosomal Inheritance; D006367:HeLa Cells; D006801:Humans; D009693:Nucleic Acid Hybridization; D012150:Polymorphism, Restriction Fragment Length
D004270:DNA, Circular; D004276:DNA, Satellite; D003513:Cycloheximide
Kharakteristika klonirovannykh al'fa-satellitnykh posledovatel'nosteĭ iz ékstrakhromosomnykh DNK kul'tur kletok HeLa.