High density culture of hybridoma cells using a perfusion culture vessel with an external centrifuge.
The influence of centrifugal force on the growth of cells was examined by exposing the cells of the mouse-human hybridoma X87 line to centrifugal force (100-500 G) for ten minutes twice a day and comparing the static culture with that of unexposed cells. In this experiment, both cell proliferation and specific antibody productivity were independent of the centrifugal effect, and gave the same results as in the case of no exposure to centrifugal force. High density cultivation of the mouse-human hybridoma X87 line was obtained by a perfusion system where the cells were separated from the culture medium by continuous centrifugation. In the serum-free culture, the maximum viable cell density exceeded 10(7) cells/ml, and monoclonal antibody was stably produced for 37 days. The results in this culture were equivalent to those obtained by intermittent centrifugal cell separation from the culture medium, and separation by gravitational settlement.
Biotechnology Research Laboratories, Tokyo Research Center, Japan.
M
M
Tokashiki
T
T
Arai
K
K
Hamamoto
K
K
Ishimaru
D000818:Animals; D002469:Cell Separation; D002498:Centrifugation / Q000295:instrumentation; D003470:Culture Media; D006801:Humans; D006825:Hybridomas / Q000166:cytology*; D008722:Methods; D010477:Perfusion / Q000295:instrumentation
D003470:Culture Media