A comparative study of the performance of solid supported and soluble urease for the enzymatic hydrolysis of urea.
The performance of both free and solid supported urease for the enzymatic hydrolysis of urea was studied. Kinetic analysis of reaction rate data shows that the kinetic data were consistent with the proposed model. Statistical tests validated the model. Despite the deactivation of ureases, the immobilized enzyme could be the choice for a technological process aiming at the detoxification of blood in haemoperfusion columns.
Demirel
G
G
Department of Chemistry, Middle East Technical University, Ankara, Turkey.
Akovali
G
G
Tanyolac
A
A
Hasirci
N
N
D001709:Biotechnology; D002606:Charcoal; D004058:Diffusion; D004800:Enzymes, Immobilized / Q000378:metabolism*; D006868:Hydrolysis; D066298:In Vitro Techniques; D007700:Kinetics; D008956:Models, Chemical; D012995:Solubility; D014508:Urea / Q000378:metabolism*; D014510:Urease / Q000378:metabolism*
D004800:Enzymes, Immobilized; D002606:Charcoal; D014508:Urea; D014510:Urease