Effects of i.c.v. lithium chloride administration on monoamine concentration in rat mediobasal hypothalamus.
We investigated the acute effects of a single i.c.v. injection of lithium chloride (LiCl) the neuroamine content of the rat mediobasal hypothalamus (MBH). The effects of lithium on amine synthesis and degradation enzymes were also studied in vitro. Noradrenaline (NA), dopamine (DA), serotonin (5-HT) and 5-hydroxyindoleacetic acid (5-HIAA) concentrations were reduced 10 min after i.c.v. injection of 24 nmol of LiCl and returned to control values 30 min after the injection. Two nmol of LiCl reduced the concentration of DA (10 and 30 min after injection) and 5-HIAA (30 min after injection). LiCl (0.5-10 mM) inhibited tyrosine hydroxylase activity (catecholamine synthesis) in vitro in a concentration dependent manner. The i.c.v. administration of a high dose of LiCl reduced the content of neuroamines in the MBH. This might result from and inhibition of synthesis. A possible link between the observed changes and some reported side effects of lithium therapy is discussed.
Otero Losada
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Instituto de Investigaciones Farmacológicas, Consejo Nacional de Investigaciones Científicas y Técnicas (ININFA) (CONICET), Buenos Aires, Argentina.
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D000818:Animals; D015306:Biogenic Monoamines / Q000378:metabolism*; D002712:Chlorides / Q000008:administration & dosage* / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D004563:Electrochemistry; D007033:Hypothalamus, Middle / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D007276:Injections, Intraventricular; D008094:Lithium / Q000008:administration & dosage* / Q000494:pharmacology; D018021:Lithium Chloride; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
D015306:Biogenic Monoamines; D002712:Chlorides; D008094:Lithium; D014446:Tyrosine 3-Monooxygenase; D018021:Lithium Chloride