A novel human hepatoblastoma cell line (HuH-6KK) with rapid growth in serum-free medium without extracellular matrix.
A novel human hepatoblastoma cell line (HuH-6KK) with a high growth rate in a serum-free medium without extracellular matrix was developed from an original one, HuH-6 c15 (HuH). The original HuH cells (38 passages) did not proliferate well in RPMI 1640 medium containing 20% fetal calf serum (FCS). The HuH cells (HuH-6KK) with a high growth rate were selected by culturing them in an enriched RDF containing 20% FCS and 0.01% mucous polysaccharide (spirulinan) isolated from a blue-green alga, Spirulina subsalsa. The HuH-6KK cells showed a rapid growth in serum-free eRDF medium containing insulin, transferrin, ethanolamine, and selenite (eRDF-ITES medium) without fibronectin. The proliferation of the original HuH cells was also observed in the eRDF-ITES medium, but the growth was slow compared with the HuH-6KK cells. In the medium without ITES, the growth of the HuH-6KK and original HuH cells was slow. Among the ITES ingredients, insulin promoted the growth of HuH-6KK cells the most.
National Food Research Institute, Ibaraki, Japan.
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Shinohara
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Kong
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Nagamine
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Shiraishi
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Murakami
D001769:Blood; D002133:Calcium Sulfate / Q000494:pharmacology; D006528:Carcinoma, Hepatocellular / Q000473:pathology*; D002455:Cell Division / Q000187:drug effects; D003470:Culture Media; D019856:Ethanolamine; D004983:Ethanolamines / Q000494:pharmacology; D005109:Extracellular Matrix / Q000502:physiology; D006801:Humans; D007328:Insulin / Q000494:pharmacology; D008074:Lipoproteins / Q000494:pharmacology; D008113:Liver Neoplasms / Q000473:pathology*; D011134:Polysaccharides; D014168:Transferrin / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D003470:Culture Media; D004983:Ethanolamines; D007328:Insulin; D008074:Lipoproteins; D011134:Polysaccharides; D014168:Transferrin; C068071:lambda spirulinan; D019856:Ethanolamine; D002133:Calcium Sulfate