Adipogenic cell line TA1: a suitable model to study the effect of beta-adrenergic agonists on lipid metabolism.
The stable adipogenic cell line TA1 was investigated as a potential in vitro system to examine effects of beta-adrenergic agonists on lipid metabolism at the cellular level. Initial experiments were conducted to establish whether dexamethasone, indomethacin, or both in combination induce rapid differentiation of TA1 preadipocytes to adipocytes. Based on activity of fatty acid synthase, dexamethasone and indomethacin, individually and in combination, were observed to induce differentiation in TA1 cells at different rates (dexamethasone/indomethacin greater than indomethacin greater than dexamethasone). Dexamethasone/indomethacin induced complete differentiation in TA1 cells 4 days after confluence, as indicated by increased activity of fatty acid synthase, glycerol-3-phosphate dehydrogenase, and malic enzyme. Finally, mature TA1 adipocytes were treated with various concentrations of isoproterenol and ractopamine to determine the responsiveness of TA1 adipocytes to a beta-adrenergic challenge. Glycerol release was increased and fatty acid synthase activity was decreased in a dose-dependent manner for both isoproterenol and ractopamine. These results indicate that fully differentiated TA1 adipocytes may be useful to study direct cellular effects of lipolytic and lipogenic agents on lipid metabolism.
Department of Animal Science, Michigan State University, East Lansing 48824-1225.
P S
PS
Weber
R A
RA
Merkel
W G
WG
Bergen
D000273:Adipose Tissue / Q000378:metabolism*; D000318:Adrenergic beta-Agonists / Q000494:pharmacology*; D000704:Analysis of Variance; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D003907:Dexamethasone / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D064429:Fatty Acid Synthases / Q000096:biosynthesis; D005990:Glycerol / Q000378:metabolism; D005993:Glycerolphosphate Dehydrogenase / Q000096:biosynthesis; D007213:Indomethacin / Q000494:pharmacology; D007545:Isoproterenol / Q000494:pharmacology*; D050356:Lipid Metabolism; D008291:Malate Dehydrogenase / Q000096:biosynthesis; D051379:Mice; D010627:Phenethylamines / Q000494:pharmacology*
D000318:Adrenergic beta-Agonists; D010627:Phenethylamines; C060897:ractopamine; D003907:Dexamethasone; D005993:Glycerolphosphate Dehydrogenase; D008291:Malate Dehydrogenase; D064429:Fatty Acid Synthases; D007545:Isoproterenol; D005990:Glycerol; D007213:Indomethacin