Production of 5'-ribonucleotides by enzymatic hydrolysis of RNA.
Study of optimal operational conditions for RNA enzymatic hydrolysis to obtain 5'-ribonucleotides has been carried out. RNA from brewer's yeasts, obtained by ammonium extraction, was hydrolysed by a partially purified 5'-phosphodiesterase from barley rootlets. Temperature of 60 degrees C and pH 7 have been determined as the best operational conditions. Low RNA initial concentration (approximately 0.1%) and reaction time (approximately 1 h) have been identified as necessary to obtain a good yield of 5'-ribonucleotides.
Facultat de Ciències, Universitat Autònoma de Barcelona, Bellaterra, Spain.
M D
MD
Benaiges
J
J
López-Santin
C
C
Solà
D006868:Hydrolysis; D007700:Kinetics; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism*; D012331:RNA, Fungal / Q000302:isolation & purification; D012265:Ribonucleotides / Q000302:isolation & purification; D012441:Saccharomyces cerevisiae
D012331:RNA, Fungal; D012265:Ribonucleotides; D010744:Phosphoric Monoester Hydrolases