Heritable damage to yeast caused by transformation.
The introduction of plasmid DNA into yeast by transformation or electroporation, but not by cytoduction, results in the induction of a slow growth phenotype. This phenotype is inherited as a dominant Mendelian trait, which is only exhibited in the absence of the native 2 mu nuclear DNA plasmid of yeast. The use of recombinant DNA technology in yeast, therefore, does not necessarily manipulate the genome in a precise and completely defined way.
Department of Biochemistry and Applied Molecular Biology, University of Manchester Institute of Science and Technology, UK.
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N
Danhash
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Gardner
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Oliver
D004247:DNA / Q000235:genetics; D010641:Phenotype; D010957:Plasmids; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000254:growth & development*; D012641:Selection, Genetic; D014170:Transformation, Genetic
D004247:DNA