Segregation of human T cell lymphotropic virus type I and II infections by antibody reactivity to unique viral epitopes.
A recombinant protein of the human T cell lymphotropic virus type I (HTLV-I) gp46 outer membrane envelope, MTA-4 (residues 129-203), reacted by Western blot with sera from HTLV-I-infected individuals from the United States and Jamaica but not with 24 (10%) of 242 Japanese sera. A related gp46 recombinant protein, MTA-1 (residues 162-209), reacted with all 58 sera from HTLV-I-infected US and Jamaican individuals and 238 of 242 sera from infected Japanese (combined sensitivity of 99%). Neither recombinant showed reactivity to sera from HTLV-II-infected individuals or uninfected controls. The reactivity of recombinant proteins containing the region of HTLV-II gp46 analogous to MTA-1 was also evaluated by Western blot: GH2-K15 (residues 157-205) and GH2-K55 (residues 162-205) reacted with 88 (98%) and 89 (99%), respectively, of 90 sera from HTLV-II-infected individuals but not with sera from HTLV-I-infected individuals or uninfected controls. These recombinant proteins should permit the development of assays to unambiguously confirm and differentiate HTLV-I and HTLV-II infections.
Department of Pathology, Stanford University School of Medicine, CA.
J J
JJ
Lipka
I
I
Miyoshi
K G
KG
Hadlock
G R
GR
Reyes
T P
TP
Chow
W A
WA
Blattner
G M
GM
Shaw
C V
CV
Hanson
D
D
Gallo
L
L
Chan
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal; D015153:Blotting, Western; D015480:Deltaretrovirus Antibodies / Q000096:biosynthesis*; D003937:Diagnosis, Differential; D000939:Epitopes / Q000737:chemistry / Q000276:immunology; D015486:HTLV-I Antigens / Q000737:chemistry / Q000276:immunology*; D015490:HTLV-I Infections / Q000175:diagnosis*; D015487:HTLV-II Antigens / Q000737:chemistry / Q000276:immunology*; D015491:HTLV-II Infections / Q000175:diagnosis*; D006801:Humans; D007563:Jamaica; D007564:Japan; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000737:chemistry / Q000276:immunology; D014481:United States
D000911:Antibodies, Monoclonal; D015480:Deltaretrovirus Antibodies; D000939:Epitopes; D015486:HTLV-I Antigens; D015487:HTLV-II Antigens; D011994:Recombinant Proteins