Potential- and acetylcholine-activated ionic currents of pheochromocytoma PC12 cells during incubation with nerve growth factor.
Pheochromocytoma PC12 cells incubated with and without nerve growth factor were investigated using the patch-clamp technique in the whole-cell recording mode, and the concentration clamp method in the rat. On the fourth day of incubation with nerve growth factor, sodium potential-activated ionic currents appeared in the membranes of the most morphologically differentiated cells. At the same period a three-fold increase of acetylcholine-activated current density, compared with the cells incubated without nerve growth factor, was observed. Thus, the qualitative and quantitative changes in membrane properties can be a result of metabolic reorganization in PC12 cells induced by nerve growth factor and accompanied by morphological differentiation according to neuronal phenotype.
Baev
K V
KV
Department of Physiology of the Spinal Cord, A. A. Bogomoletz Institute of Physiology, Academy of Sciences, Ukrainian SSR, Kiev.
Beresovskii
V K
VK
Kalunov
V N
VN
Luschitskaya
N I
NI
Rusin
K I
KI
Vilner BYa
Zavadskaya
T V
TV
D000109:Acetylcholine / Q000494:pharmacology*; D000310:Adrenal Gland Neoplasms; D000818:Animals; D004305:Dose-Response Relationship, Drug; D005071:Evoked Potentials / Q000187:drug effects; D007473:Ion Channels / Q000187:drug effects / Q000502:physiology*; D007700:Kinetics; D009414:Nerve Growth Factors / Q000494:pharmacology*; D016716:PC12 Cells; D010673:Pheochromocytoma; D015221:Potassium Channels / Q000187:drug effects / Q000502:physiology; D015222:Sodium Channels / Q000187:drug effects / Q000502:physiology; D013779:Tetrodotoxin / Q000494:pharmacology; D013997:Time Factors
D007473:Ion Channels; D009414:Nerve Growth Factors; D015221:Potassium Channels; D015222:Sodium Channels; D013779:Tetrodotoxin; D000109:Acetylcholine