Screening of staphylococci for the toxic shock syndrome toxin-1 (TSST-1) gene.
Dot blot hybridization was used to screen 820 staphylococci for the presence of the gene coding for TSST-1. The DNA of 33 strains among 70 Staph. aureus strains isolated from suspected toxic shock syndrome (TSS) cases hybridized with the probe. These results agreed perfectly with those obtained with a phenotypic method (immunodiffusion). Among 608 Staph. aureus strains isolated over a period of one month from hospitalized patients, 66 (11%) hybridized with the probe; of these strains, 64 (97%) were found to produce TSST-1 in vitro. None of 145 coagulase-negative staphylococcal strains harboured DNA hybridizing with the probe. The data indicate that this genotypic assay is suitable for epidemiological studies.
Jaulhac
B
B
Institut de Bactériologie, Université Louis Pasteur, Strasbourg, France.
De Buyser
M L
ML
Dilasser
F
F
Prevost
G
G
Piedmont
Y
Y
D001427:Bacterial Toxins; D001483:Base Sequence; D015342:DNA Probes / Q000737:chemistry; D004269:DNA, Bacterial / Q000032:analysis* / Q000737:chemistry; D004768:Enterotoxins / Q000032:analysis / Q000235:genetics*; D006801:Humans; D005779:Immunodiffusion; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D015203:Reproducibility of Results; D012189:Retrospective Studies; D012772:Shock, Septic / Q000382:microbiology*; D013203:Staphylococcal Infections / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000235:genetics*; D018089:Superantigens
D001427:Bacterial Toxins; D015342:DNA Probes; D004269:DNA, Bacterial; D004768:Enterotoxins; D018089:Superantigens; C030107:enterotoxin F, Staphylococcal