Improved microscopic observation of mammalian cells on microcarriers by fluorescent staining.
Many microcarriers used for the cultivation of animal cells do not allow for convenient microscopic observation of cell morphology and viability due to their optical properties. Using fluorescent viable stain combining fluorescein diacetate and ethidium bromide, we observed the distribution, morphology and viability of cells on various microcarriers.
Nikolai
T J
TJ
Department of Chemical Engineering and Materials Science, University of Minnesota, Minneapolis 55455.
Peshwa
M V
MV
Goetghebeur
S
S
Hu
W S
WS
D000818:Animals; D002455:Cell Division; D002460:Cell Line / Q000166:cytology*; D002470:Cell Survival; D006224:Cricetinae; D004996:Ethidium; D005452:Fluoresceins; D005456:Fluorescent Dyes; D008863:Microspheres; D014709:Vero Cells
D005452:Fluoresceins; D005456:Fluorescent Dyes; D004996:Ethidium; C018506:diacetylfluorescein