Time-dependent alterations of leukotriene production and catabolism in rat peritoneal macrophages following intraperitoneal injection of thioglycollate broth.
Alterations of leukotriene (LT) productivity in peritoneal macrophages (PM) from untreated rats (control) as well as from rats treated i.p. with thioglycollate broth (TG) were investigated on days 3, 7 and 14 after TG administration. The resident PM from the untreated rats produced mainly LTB4 and 5-HETE with small amounts of 12-HETE and LTD4 with only a trace of LTC4 when stimulated with the calcium ionophore A23187. The PM elicited from rats on days 3 and 7 produced more LTC4 than did the resident PM but fewer other lipoxygenase metabolites. On day 14, however, the elicited PM resembled the resident PM in terms of lipoxygenase metabolite production. Similar results were achieved in the presence of arachidonic acid and A23187. A decrease in lipoxygenase metabolism in the elicited PM was also suggested by using opsonized zymosan. Catabolism studies indicated a reduction in r-glutamyl transpeptidase activity in the elicited PM and suggested a reduction in catabolism for LTB4 in the former cells. The authors conclude that the TG-elicited PM generate fewer lipoxygenase metabolites than the resident PM following stimulation, but show a preferential conversion of LTA4 to sulfidopeptide LTs rather than to LTB4. The elicited PM also show a reduced catabolism for LTC4 and LTB4.
Research Institute for Diseases of the Chest, Medical School of Kyushu University, Fukuoka, Japan.
M
M
Abe
T
T
Goya
N
N
Nagata
H
H
Takahashi
H
H
Muranishi
N
N
Shigematsu
D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism / Q000494:pharmacology; D000001:Calcimycin / Q000494:pharmacology; D002452:Cell Count; D007975:Leukotriene B4 / Q000378:metabolism*; D008262:Macrophage Activation / Q000187:drug effects*; D008264:Macrophages / Q000378:metabolism*; D008297:Male; D008563:Membrane Lipids / Q000378:metabolism; D010538:Peritonitis / Q000139:chemically induced / Q000473:pathology*; D051381:Rats; D011919:Rats, Inbred Strains; D013189:SRS-A / Q000378:metabolism*; D013864:Thioglycolates / Q000633:toxicity; D013997:Time Factors; D015054:Zymosan / Q000494:pharmacology; D005723:gamma-Glutamyltransferase / Q000378:metabolism
D008563:Membrane Lipids; D013189:SRS-A; D013864:Thioglycolates; D007975:Leukotriene B4; D016718:Arachidonic Acid; D000001:Calcimycin; D015054:Zymosan; D005723:gamma-Glutamyltransferase