Proliferating cell nuclear antigen counts in formalin-fixed paraffin-embedded tissue correlate with Ki-67 in fresh tissue.
Cell proliferation can be studied by a variety of techniques. However, most require fresh tissue. To evaluate cell proliferation in formalin-fixed paraffin-embedded sections, the authors immunohistochemically studied 35 tumors and 11 samples of normal/hyperplastic tissue with PC10, a monoclonal antibody directed against proliferating cell nuclear antigen. Results were compared with those obtained with Ki-67 on fresh tissues. There was no significant difference between proliferating cell nuclear antigen and Ki-67 counts, which were strongly correlated (r = 0.8). Proliferating cell nuclear antigen positivity was easier to evaluate because morphology was better preserved in formalin-fixed tissue. The authors conclude that PC10 is an alternative to Ki-67 in evaluating cell proliferation and has the advantage of reacting with formalin-fixed paraffin-embedded tissue.
Department of Pathology, Mater Hospital, Dublin, Ireland.
P A
PA
Dervan
H M
HM
Magee
C
C
Buckley
D N
DN
Carney
D000911:Antibodies, Monoclonal / Q000276:immunology*; D000951:Antigens, Neoplasm / Q000032:analysis; D005404:Fixatives; D005557:Formaldehyde; D006801:Humans; D019394:Ki-67 Antigen; D009369:Neoplasms / Q000276:immunology / Q000473:pathology; D009687:Nuclear Proteins / Q000032:analysis* / Q000276:immunology*; D016612:Paraffin Embedding; D018809:Proliferating Cell Nuclear Antigen; D012016:Reference Values
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D005404:Fixatives; D019394:Ki-67 Antigen; D009687:Nuclear Proteins; D018809:Proliferating Cell Nuclear Antigen; D005557:Formaldehyde