Human endometrial stromal cells and decidual cells express cluster of differentiation (CD) 13 antigen/aminopeptidase N and CD10 antigen/neutral endopeptidase.
With specific monoclonal antibodies, we found that human endometrial stromal cells and decidual cells express two function-related surface antigens. Indirect immunofluorescence staining revealed that both endometrial stromal cells and decidual cells during the first trimester of pregnancy expressed cluster of differentiation (CD) 13 antigen and CD10 antigen, which are identical to aminopeptidase N and neutral endopeptidase, respectively. By flow cytometric analysis, CD13 antigen was detected on 82-93% of the examined cells, and CD10 antigen was detected on 75-93% of the examined cells in endometrial stromal cell-enriched preparations. Furthermore, peptidase activity was detected in these cell preparations by an assay based on the hydrolysis of alanine-p-nitroanilide into p-nitroaniline and alanine.
Department of Gynecology and Obstetrics, Faculty of Medicine, Kyoto University, Japan.
K
K
Imai
M
M
Maeda
H
H
Fujiwara
N
N
Okamoto
M
M
Kariya
N
N
Emi
K
K
Takakura
H
H
Kanzaki
T
T
Mori
D000328:Adult; D000409:Alanine / Q000378:metabolism; D000626:Aminopeptidases / Q000378:metabolism*; D000814:Aniline Compounds / Q000378:metabolism; D015703:Antigens, CD / Q000032:analysis*; D000943:Antigens, Differentiation / Q000032:analysis*; D015214:Antigens, Differentiation, Myelomonocytic / Q000032:analysis*; D000951:Antigens, Neoplasm / Q000032:analysis*; D018826:CD13 Antigens; D003656:Decidua / Q000166:cytology / Q000201:enzymology* / Q000276:immunology; D004717:Endometrium / Q000166:cytology / Q000201:enzymology* / Q000276:immunology; D005260:Female; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D006801:Humans; D008875:Middle Aged; D015260:Neprilysin; D011247:Pregnancy
D000814:Aniline Compounds; D015703:Antigens, CD; D000943:Antigens, Differentiation; D015214:Antigens, Differentiation, Myelomonocytic; D000951:Antigens, Neoplasm; C004813:alanine-4-nitroanilide; C019498:4-nitroaniline; D000626:Aminopeptidases; D018826:CD13 Antigens; D015260:Neprilysin; D000409:Alanine