Structure and RNA content of the prosomes.
Duck erythroblasts prosomes were analysed by small angle neutron scattering (SANS), dynamic light scattering and (cryo-)electron microscopy. A molecular weight of approximately 720,000 +/- 50,000, a radius of gyration of 64 +/- 2 A and a hydrodynamic radius of approximately 86 A were obtained. Electron micrographs show a hollow cylinder-like particle with a diameter of 120 A, a height of 170 A and a diameter of 40 A for the cavity, built of four discs, the two outer ones being more pronounced than those in the center. Results from SANS indicate less then 5% of RNA in the purified prosomes, but nuclease protection assays confirm its presence.
Institut Jacques Monod, Paris, France.
O
O
Coux
H G
HG
Nothwang
K
K
Scherrer
W
W
Bergsma-Schutter
A C
AC
Arnberg
P A
PA
Timmins
J
J
Langowski
C
C
Cohen-Addad
D000818:Animals; D004372:Ducks; D004591:Electrophoresis, Polyacrylamide Gel; D004912:Erythrocytes / Q000737:chemistry / Q000648:ultrastructure; D008027:Light; D008854:Microscopy, Electron; D009502:Neutrons; D012313:RNA / Q000737:chemistry* / Q000648:ultrastructure; D012260:Ribonucleases / Q000378:metabolism; D012261:Ribonucleoproteins / Q000235:genetics*; D012542:Scattering, Radiation
D012261:Ribonucleoproteins; D012313:RNA; D012260:Ribonucleases