Use of monoclonal anti-enzyme antibodies for analytical purposes.
This review discusses the analytical applications of monoclonal antibodies specific for enzymes. One important, but not well-studied, application of these monoclonal antibodies is their use in immobilizing enzymes on solid supports. This method is based on binding the enzymes to an immobilized antibody through the antigen binding site of the antibody. Enzymes immobilized this way retain much of their activity. The utility of immobilized enzyme reactors prepared by immobilizing the enzymes through antibodies is demonstrated by using them in the determination of acetylcholine and choline in brain tissue extracts. Currently available methods for immobilizing antibodies and enzymes are reviewed. Other issues discussed in this review include the problems and advantages of immobilized enzyme reactors, especially when used in conjunction with HPLC. In addition, the applications of monoclonal antibodies for the detection and measurement of enzymes and their isoforms are summarized.
Gunaratna
P C
PC
Department of Chemistry, University of Kansas, Lawrence 66045.
Wilson
G S
GS
D000109:Acetylcholine / Q000032:analysis; D000911:Antibodies, Monoclonal; D002851:Chromatography, High Pressure Liquid; D004798:Enzymes / Q000276:immunology*; D004800:Enzymes, Immobilized
D000911:Antibodies, Monoclonal; D004798:Enzymes; D004800:Enzymes, Immobilized; D000109:Acetylcholine