A new source of biologically active compounds--earthworm tissue (Eisenia foetida, Lumbricus rubelus).
1. Earthworms possess immunological recognition and memory as well as high regenerative abilities. Coelomic fluid was used as a source of biologically active compounds. 2. A biologically active glycolipoprotein extract from a whole earthworm tissue homogenate was isolated and named G-90. 3. G-90 forms precipitation arcs in gel with different animal and human sera. 4. It alters murine cell growth rate in vitro in serum in a dose-dependent manner and slows murine tumor growth in vivo. 5. G-90 does not contain mutagens or carcinogens.
Department of Biology, Faculty of Veterinary Medicine, University of Zagreb, Croatia.
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Hrzenjak
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Hrzenjak
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Kasuba
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Efenberger-Marinculić
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S
Levanat
D000818:Animals; D002855:Chromatography, Thin Layer; D004591:Electrophoresis, Polyacrylamide Gel; D004912:Erythrocytes / Q000187:drug effects; D006023:Glycoproteins / Q000302:isolation & purification / Q000494:pharmacology*; D006386:Hemagglutination Tests; D006801:Humans; D005779:Immunodiffusion; D007525:Isoelectric Focusing; D008074:Lipoproteins / Q000302:isolation & purification / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D009835:Oligochaeta / Q000737:chemistry*; D013055:Spectrophotometry, Infrared; D014407:Tumor Cells, Cultured
D006023:Glycoproteins; D008074:Lipoproteins