A molecular model for illegitimate recombination in Bacillus subtilis.
The recombinant DNA junctions at which pUB110 and Bacillus subtilis chromosomal DNA were joined to form the plasmid pKBT1 were cloned and sequenced. From the sequencing data we conclude that the pUB110 sequence is intact in the pair of cloned pKBT1 fragments and pTL12 sequences are not present. A molecular model for the formation of pKBT1 based on structural motifs characteristic of the joint sites is presented.
Agricultural Research Service, US Department of Agriculture, Kerrville, TX 78028.
K B
KB
Temeyer
K M
KM
Hopkins
L F
LF
Chapman
D001412:Bacillus subtilis / Q000235:genetics*; D001426:Bacterial Proteins; D001483:Base Sequence; D003001:Cloning, Molecular; D004250:DNA Topoisomerases, Type II / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics*; D004274:DNA, Recombinant; D015245:Deoxyribonuclease BamHI; D015252:Deoxyribonucleases, Type II Site-Specific; D008969:Molecular Sequence Data; D010957:Plasmids
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004274:DNA, Recombinant; D015245:Deoxyribonuclease BamHI; C024404:BglII endonuclease; D015252:Deoxyribonucleases, Type II Site-Specific; D004250:DNA Topoisomerases, Type II