Serum-free medium for fermentor cultures of hybridomas.
Hybridomas lend themselves particularly well to large scale cultivation techniques since they grow as single cells in suspension without requiring attachment to a substrate. Furthermore, many cell strains have been adapted to grow in serum-free (SF) media to a similar cell density and antibody production as in serum containing media. This review will concern itself mainly with the cultivation of hybridomas in SF-media in bioreactors of various types with the ultimate goal of producing large quantities of monoclonal antibodies (mAb).
Merten
O W
OW
Institut Pasteur, Laboratoire de Technologie Cellulaire, Paris, France.
Litwin
J
J
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D001769:Blood; D002455:Cell Division; D003470:Culture Media; D005285:Fermentation; D006801:Humans; D006825:Hybridomas; D051379:Mice
D000911:Antibodies, Monoclonal; D003470:Culture Media