DNA synthesis control in yeast: an evolutionarily conserved mechanism for regulating DNA synthesis genes?
After yeast cells commit to the cell cycle in a process called START, genes required for DNA synthesis are expressed in late G1. Periodicity is mediated by a hexameric sequence, known as a MCB element, present in all DNA synthesis gene promoters. A complex that specifically binds MCBs has been identified. One polypeptide in the MCB complex is Swi6, a transcription factor that together with Swi4 also binds G1 cyclin promoters and participates in a positive feedback loop at START. The finding that Swi6 is directly involved in both START and DNA synthesis gene control suggest a model in which Swi6, activated through its participation in START, serves as the central transcription factor in coordinating late G1 gene expression. The mechanism may be conserved in all eukaryotic cells.
Department of Biochemistry and Biophysics, Oregon State University, Corvallis 97331.
G F
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Merrill
B A
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Morgan
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Lowndes
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Johnston
D000818:Animals; D001483:Base Sequence; D002453:Cell Cycle / Q000235:genetics; D004261:DNA Replication / Q000235:genetics; D004271:DNA, Fungal / Q000096:biosynthesis* / Q000235:genetics; D005246:Feedback; D005656:Fungal Proteins / Q000235:genetics / Q000502:physiology; D005800:Genes, Fungal; D008957:Models, Genetic; D008969:Molecular Sequence Data; D009154:Mutation; D018741:Replication Origin; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D014157:Transcription Factors / Q000235:genetics / Q000502:physiology; D014714:Vertebrates / Q000235:genetics
D004271:DNA, Fungal; D005656:Fungal Proteins; D014157:Transcription Factors