Gene structure of Bombyx mori larval serum protein (BmLSP).
To understand the molecular mechanisms of the larval-specific transcription of Bombyx mori larval serum protein (BmLSP), we isolated a clone of the BmLSP gene from a genomic library and sequenced a 3.5-kb fragment. An intron was found in the 5' noncoding region of the BmLSP gene. A putative transcription start point was determined by primer extension analysis. Genomic Southern hybridization showed that there is one copy of the BmLSP gene in a haploid genome. A database search revealed that the BmLSP gene has presumptive repetitive sequences found in other B. mori genes, the sequence homologous to ecdysone-responsive elements and a heptamer sequence found in storage protein genes.
Laboratory of Sericultural Science, School of Agriculture, Nagoya University, Japan.
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Fujiwara
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Yamashita
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D012831:Bombyx / Q000737:chemistry / Q000235:genetics*; D004261:DNA Replication; D029721:Drosophila Proteins; D017344:Genes, Insect; D007301:Insect Hormones / Q000235:genetics* / Q000302:isolation & purification; D007814:Larva / Q000737:chemistry / Q000235:genetics; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
D029721:Drosophila Proteins; D007301:Insect Hormones; C034648:larval serum protein, Drosophila