The effect of neutral resins on the fermentation production of rubradirin.
Rubradirin is an antibiotic of complex chemical structure which is active vs. methicillin resistant staphylococci. Its development has been limited due to inadequate production yields. The incorporation of neutral resins into fermentations of Streptomyces achromogenes v. rubradiris, UC 8051 resulted in the enhanced production of rubradirin. Resins HP-20, HP-21, XAD-2, XAD-7 and XAD-16 were employed in flask and tank fermentations. The incorporation of these resins promoted 2- to 4-fold enhancements of the rubradirin activity produced in flask fermentations, and the incorporation of XAD-16 and HP-21 into tank fermentations promoted production titer increases greater than 5 fold.
Marshall
V P
VP
Research Laboratories, Upjohn Company, Kalamazoo, MI 49001.
McWethy
S J
SJ
Sirotti
J M
JM
Cialdella
J I
JI
D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000737:chemistry / Q000494:pharmacology; D001681:Biological Assay; D003470:Culture Media; D005285:Fermentation; D008837:Micrococcus / Q000187:drug effects; D015394:Molecular Structure; D009285:Naphthoquinones / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D012116:Resins, Plant / Q000494:pharmacology*; D013302:Streptomyces / Q000378:metabolism*
D000900:Anti-Bacterial Agents; D003470:Culture Media; D009285:Naphthoquinones; D012116:Resins, Plant; C002857:rubradirin