The effects of interferon-beta on phorbol ester or calcium ionophore-induced intercellular adhesion molecule-I expression in epidermal carcinoma cells.
Keratinocyte intercellular adhesion molecule (ICAM)-I expression is induced by interferon (IFN)-gamma. It has been previously reported that IFN-beta suppresses IFN-gamma-induced ICAM-I expression in A431 cells, a human squamous cell carcinoma cell line. In this study, the suppression mechanisms were investigated at the post second messenger level. Both 12-O-tetradecanoylphorbol-13-acetate (TPA) and calcium ionophore (A23187) induce ICAM-I expression in A431 cells. ICAM-I expression induced by either was not suppressed with cotreatment with IFN-beta. Furthermore, IFN-beta did not inhibit the translocation of protein kinase C (PKC) by TPA. It appears that the pathways involved in ICAM-I expression induced by activation of PKC or increased in intracellular Ca++ are not affected by IFN-beta.
Fujisawa
H
H
Department of Dermatology, University of Tsukuba, Ibaraki Prefecture, Japan.
Naito
Y
Y
Horiuchi
S
S
Baba
T
T
Otsuka
F
F
D000001:Calcimycin / Q000494:pharmacology*; D002294:Carcinoma, Squamous Cell / Q000201:enzymology / Q000276:immunology / Q000473:pathology*; D015815:Cell Adhesion Molecules / Q000187:drug effects / Q000276:immunology*; D002460:Cell Line; D004789:Enzyme Activation / Q000187:drug effects / Q000502:physiology; D006801:Humans; D066298:In Vitro Techniques; D018799:Intercellular Adhesion Molecule-1; D016899:Interferon-beta / Q000276:immunology*; D015603:Keratinocytes / Q000187:drug effects / Q000201:enzymology / Q000276:immunology*; D011493:Protein Kinase C / Q000378:metabolism*; D015290:Second Messenger Systems / Q000187:drug effects; D012878:Skin Neoplasms / Q000201:enzymology / Q000276:immunology / Q000473:pathology*; D013755:Tetradecanoylphorbol Acetate / Q000031:analogs & derivatives* / Q000494:pharmacology
D015815:Cell Adhesion Molecules; D018799:Intercellular Adhesion Molecule-1; D000001:Calcimycin; C032975:12-O-tetradeca-2,4,6,8-tetranoylphorbol-13-acetate; D016899:Interferon-beta; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate