A CHO strain producing high-level human IL-6 with the 3' deletion construct.
A Chinese Hamster Ovary (CHO) strain producing high-level human interleukin 6 (IL-6) was obtained, by introduction of the IL-6 cDNA from which the 3' AU-rich region was deleted. The IL-6 mRNA of this strain was stable. The productivity was more than 15 times higher than the previously obtained clone, which has intact IL-6 cDNA in the same expression vector. This strain produced IL-6 at a high level of 30 micrograms ml-1 in batch culture, or a continuous 20 micrograms per 10(6) cells per day in microcarrier cultivation.
Tonouchi
N
N
Central Research Laboratories, Ajinomoto Co. Inc., Kawasaki, Japan.
Koyama
N
N
Miwa
K
K
D000818:Animals; D015152:Blotting, Northern; D015139:Blotting, Southern; D016466:CHO Cells; D003001:Cloning, Molecular; D006224:Cricetinae; D005784:Gene Amplification; D006801:Humans; D015850:Interleukin-6 / Q000235:genetics* / Q000378:metabolism; D007700:Kinetics; D009154:Mutation; D012333:RNA, Messenger / Q000378:metabolism
D015850:Interleukin-6; D012333:RNA, Messenger