Technical improvements for flow karyotyping by standard FACS 440 flow cytometer.
Chromosomal DNA measurements represent the most accurate methodology for the quantitative analysis of human karyotype. A modified FACS 440 signal collection is described here. The FSC signal voltage coming from the optical bench has been split in two equal parts by a T-connector placed at the preamplifier entrance. The side scatter channel that is not employed during chromosome analysis has been used for the half-FSC signal. This modification allowed the contemporary use of FSC signal both for gating and analysis leading to a better discrimination of background, and therefore to a more accurate definition of sorting windows.
Istituto di Anatomia Umana Normale, Universitá di Bologna, Italy.
M
M
Vitale
L
L
Zamai
A
A
Antonucci
P
P
Del Grande
S
S
Papa
D002460:Cell Line; D005434:Flow Cytometry / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D007621:Karyotyping / Q000295:instrumentation / Q000379:methods*