Quantification of Moraxella bovis haemagglutinating adhesins with monoclonal antibodies.
Six monoclonal antibodies (MAbs) against Moraxella bovis GF 9 were used to quantify haemagglutinating adhesins of 16 strains of this organism. The amount of each MAb necessary to inhibit one haemagglutinating unit of each strain varied between 4 and 0.007 times that required by strain GF 9. Five strains reacted with six MAbs, one with five, two with four, one with three, two with two and three with none. The procedures used enabled to detect dominant strains candidates for vaccines.
Universidade Federal de Pelotas, Centro de Biotecnologia, RS, Brazil.
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Gil-Turnes
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Aleixo
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology; D000939:Epitopes / Q000032:analysis; D006385:Hemagglutination Inhibition Tests; D006388:Hemagglutinins / Q000032:analysis* / Q000276:immunology; D006825:Hybridomas; D007639:Keratoconjunctivitis, Infectious / Q000382:microbiology; D009016:Moraxella / Q000032:analysis* / Q000276:immunology
D000911:Antibodies, Monoclonal; D000939:Epitopes; D006388:Hemagglutinins