T cell-induced allotypic suppression. Origin of the CD8+ T cells maintaining IgG2ab suppression.
One of the problems raised by the T cell-induced allotypic suppression is the origin (donor or host) of the T cells responsible for the chronicity of the suppression. To address this point, we used T cells from Igha/a Thy-1.2 mice whose natural T cell activity against IgG2ab was enhanced in vivo. These T cells were injected into newborn Ighb/b Thy-1.1 mice where they induced complete suppression of IgG2ab expression in around 70% of these recipients. During a study that lasted more than 1 yr, we found that about 3% of the recipient splenocytes were T cells of the donor type. By means of suppression-transfer experiments, using either Thy-1.2+ or Thy-1.1+ cell-depleted splenocytes from mice suppressed in this manner we were able to unambiguously show that Thy-1.2+ cell-depleted splenocytes were incapable of transferring the suppression, whereas Thy-1.1+ cell-depleted splenocytes could. We thus demonstrated that suppression was maintained throughout the recipient's life by donor Thy-1.2+ T cells.
Unité d'Immunophysiologie Moléculaire, Institut Pasteur, Paris, France.
L
L
Majlessi
P
P
Benaroch
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G
Bordenave
D000818:Animals; D000954:Antigens, Surface / Q000032:analysis; D007108:Immune Tolerance; D007126:Immunoglobulin Allotypes / Q000276:immunology*; D007074:Immunoglobulin G / Q000276:immunology*; D008212:Lymphocyte Depletion; D008562:Membrane Glycoproteins / Q000032:analysis; D051379:Mice; D008815:Mice, Inbred Strains; D016176:T-Lymphocyte Subsets / Q000276:immunology* / Q000637:transplantation; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*; D018800:Thy-1 Antigens
D000954:Antigens, Surface; D007126:Immunoglobulin Allotypes; D007074:Immunoglobulin G; D008562:Membrane Glycoproteins; D018800:Thy-1 Antigens