Oral absorption of biologically active insulin in common carp (Cyprinus carpio L.).
1. Bovine insulin dissolved in 0.05 M deoxycholic acid was absorbed through oral intubation in fish weighing 200-300 g. 2. The peak of absorption appeared in all fish, 30-45 min after intubation and was followed by a gradual decrease. The extent of absorption was extremely variable, with up to a 20-fold difference between individuals. No detectable insulin was found in fish intubated with vehicle. 3. The absorbed hormone retained 17-88% of its lipogenic bioactivity in vitro. The absorbed insulin lowered the levels of several amino acids in the intubated fish, indirectly indicating that its bioactivity was also retained in vivo.
Hertz
Y
Y
Department of Biochemistry and Human Nutrition, Faculty of Agriculture, Hebrew University of Jerusalem, Rehovot, Israel.
Shechter
Y
Y
Madar
Z
Z
Gertler
A
A
D000042:Absorption; D000284:Administration, Oral; D000596:Amino Acids / Q000097:blood; D000818:Animals; D002347:Carps; D007328:Insulin / Q000097:blood / Q000493:pharmacokinetics* / Q000502:physiology; D009055:Mouth / Q000378:metabolism
D000596:Amino Acids; D007328:Insulin