Single tree genetic linkage mapping in conifers using haploid DNA from megagametophytes.
We report a unique application of the Random Amplified Polymorphic DNA (RAPD) technique for genetic linkage mapping of a single spruce tree using haploid DNA from megagametophyte tissue of individual seeds. Sixty-one segregating loci were analysed for reproducibility, inheritance and linkage. Forty-seven of the 61 markers were distributed into 12 linkage groups and covered 873.8 cM. The 14 markers not associated with any linkage group should be assigned to linkage groups as more markers are added to the map. This new approach quickly provides molecular genetic markers that are simple to evaluate for constructing genetic linkage maps and for other related genetic studies in forest tree species.
Biotechnology Laboratory, University of British Columbia, Vancouver, Ontario, Canada.
L K
LK
Tulsieram
J C
JC
Glaubitz
G
G
Kiss
J E
JE
Carlson
D001482:Base Composition; D001483:Base Sequence; D002874:Chromosome Mapping; D004247:DNA / Q000737:chemistry / Q000235:genetics*; D005819:Genetic Markers; D006238:Haploidy; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D010944:Plants / Q000235:genetics*; D016133:Polymerase Chain Reaction; D015183:Restriction Mapping; D012639:Seeds; D014197:Trees
D005819:Genetic Markers; D009838:Oligodeoxyribonucleotides; D004247:DNA