Flow cytometric study of hybridoma cell culture: correlation between cell surface fluorescence and IgG production rate.
Flow cytometry was applied to measure the fluorescence of IgG molecules on hybridoma cell surface stained with FITC-conjugated anti-mouse IgG F(ab')2. Using light scattering to exclude the dead cell population from the analysis, the surface fluorescence intensity allows for the differentiation between producing and nonproducing cells. A linear correlation has been found between the intensity of mean surface fluorescence of the cell population and the specific antibody production rate.
Department of Chemical Engineering and Materials Science, University of Minnesota, Minneapolis 55455.
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D000818:Animals; D002455:Cell Division; D002460:Cell Line; D002462:Cell Membrane / Q000276:immunology; D005434:Flow Cytometry / Q000379:methods; D006825:Hybridomas / Q000166:cytology / Q000276:immunology*; D007074:Immunoglobulin G / Q000032:analysis / Q000096:biosynthesis*; D007700:Kinetics; D051379:Mice; D013050:Spectrometry, Fluorescence / Q000379:methods
D007074:Immunoglobulin G