Mixed function oxidase demethylase and dealkylase activity in an electromagnetic field.
In contrast to the increase in reaction rate of microsomal NADPH-cytochrome-P450 reductase activity resulting from low-level microwave perturbation (reported earlier) transformations involving the entire MFO-pathway were inhibited by a microwave field. Dealkylation of 7-ethoxycoumarin was inhibited 25% and demethylation of p-nitroanisole was inhibited 40% when the reaction was carried forward in a 9.14 GHz CW field. Microsomal preparations from the liver of mature chickens had enzymic characteristics (kinetic constants, inhibitor-response spectrum) for these substrates similar to those reported for rodent and human MFO complex.
Chattopadhyay
S K
SK
Department of Biochemistry, NJAES, Rutgers University, New Brunswick 08903.
Brown
H D
HD
D015660:7-Alkoxycoumarin O-Dealkylase / Q000037:antagonists & inhibitors; D000818:Animals; D002645:Chickens; D004574:Electromagnetic Fields / Q000009:adverse effects*; D005260:Female; D066298:In Vitro Techniques; D008862:Microsomes, Liver / Q000201:enzymology; D006899:Mixed Function Oxygenases / Q000037:antagonists & inhibitors*; D009251:NADPH-Ferrihemoprotein Reductase / Q000378:metabolism; D010090:Oxidoreductases, O-Demethylating / Q000037:antagonists & inhibitors
C026048:4-nitroanisole O-demethylase; D006899:Mixed Function Oxygenases; D010090:Oxidoreductases, O-Demethylating; D015660:7-Alkoxycoumarin O-Dealkylase; D009251:NADPH-Ferrihemoprotein Reductase