Noradrenaline- and glutamate-induced taurine release from bulk isolated adult rat astrocytes.
The influence of noradrenaline and various agonists of glutamatergic receptors on preloaded [3H]taurine release from bulk isolated adult rat brain astrocytes was investigated by a superfusion technique. In the presence of 1 mM noradrenaline a stimulation of taurine release, resembling that observed in astroglial cultures, was preceded by an inhibition of the efflux, thus demonstrating different dynamics of noradrenaline-evoked taurine release from that observed with beta-agonists on cultured astroglia. Application of 1 mM glutamate and kainate produced stimulation of the release, while 1 mM N-methyl-D-aspartate (NMDA) and 1 mM NMDA together with 65 mM K+ had no effect on the [3H]taurine release. These data suggest the presence of kainate-sensitive and the absence of NMDA-sensitive glutamate receptors on bulk isolated astrocytes, which is consistent with previous observations on astrocytes in culture.
Puka
M
M
Department of Neurochemistry, Medical Research Centre, Polish Academy of Sciences, Warsaw.
Albrecht
J
J
Lazarewicz
J W
JW
D000818:Animals; D001253:Astrocytes / Q000187:drug effects / Q000378:metabolism*; D005971:Glutamates / Q000494:pharmacology*; D018698:Glutamic Acid; D066298:In Vitro Techniques; D009638:Norepinephrine / Q000494:pharmacology*; D051381:Rats; D013654:Taurine / Q000378:metabolism*
D005971:Glutamates; D013654:Taurine; D018698:Glutamic Acid; D009638:Norepinephrine