Chromatographic evidence for the presence of multiple tachykinins in the bovine adrenal medulla.
Among the mammalian tachykinins, substance P (SP) has been shown to be the most potent at modulating the response due to nicotinic acetylcholine receptor stimulation of bovine adrenal chromaffin cells. SP-like immunoreactivity has been detected in nerve terminals innervating the adrenal medulla; however, little is known of the presence of other tachykinins in this tissue. In this study, reverse-phase HPLC was used to fractionate peptides in bovine adrenal medullary extracts, and the fractions were analyzed by radioimmunoassay using antisera to SP or neurokinin A (NKA). The results show that both NKA- and SP-like immunoreactivities are present in the adrenal medulla. The presence of neurokinin B is also indicated. The presence of multiple tachykinins in this tissue raises questions as to their functions in the adrenal medulla.
Russell Grimwade School of Biochemistry, University of Melbourne, Parkville, Victoria, Australia.
S
S
Basile
N S
NS
Cheung
B G
BG
Livett
D000313:Adrenal Medulla / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D015288:Neurokinin A / Q000378:metabolism; D009479:Neuropeptides / Q000378:metabolism; D010446:Peptide Fragments / Q000378:metabolism; D011863:Radioimmunoassay; D013373:Substance P / Q000378:metabolism; D015320:Tachykinins / Q000737:chemistry / Q000378:metabolism*
D009479:Neuropeptides; D010446:Peptide Fragments; D015320:Tachykinins; C059905:tachykinin neuropeptide gamma; D013373:Substance P; D015288:Neurokinin A