Cephamycin C biosynthesis in Streptomyces cattleya: nitrogen source regulation.
The production of cephamycin C by Streptomyces cattleya varies with the use of asparagine, glutamine or ammonium as nitrogen sources. Hydroxylase and expandase activities were demonstrated for the first time with this species. A study of the biosynthetic regulation of these enzymes by two different nitrogen sources, glutamine and asparagine, was carried out. Asparagine proved to be a better nitrogen source, both for enzymatic biosynthesis and production of cephamycin C. Moreover, an excess of asparagine in the culture environment provokes, simultaneously, a reduction in cephamycin C production and a decrease in the biosynthesis of expandase and hydroxylase.
Laboratoire de Microbiologie Industrielle, ENSAIA, Institut National Polytechnique de Lorraine, Vandoeuvre, France.
S
S
Khaoua
A
A
Lebrihi
P
P
Germain
G
G
Lefebvre
D001120:Arginine / Q000494:pharmacology; D001216:Asparagine / Q000494:pharmacology; D002513:Cephamycins / Q000096:biosynthesis*; D019751:Intramolecular Transferases; D007535:Isomerases / Q000096:biosynthesis; D006899:Mixed Function Oxygenases / Q000096:biosynthesis; D009584:Nitrogen / Q000494:pharmacology; D046915:Penicillin-Binding Proteins; D013302:Streptomyces / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*
D002513:Cephamycins; D046915:Penicillin-Binding Proteins; D001216:Asparagine; D001120:Arginine; D006899:Mixed Function Oxygenases; D007535:Isomerases; D019751:Intramolecular Transferases; C038371:deacetoxycephalosporin C synthetase; D009584:Nitrogen; C024925:cephamycin C