A non-radiolabelled in situ hybridization method for the detection of epidermal cytokine mRNA.
We describe a non-radiolabelled method for the in situ detection of epidermal cytokine mRNA in paraffin sections of skin biopsies from sites of nickel contact sensitivity. The method uses fluorescein isothiocyanate-labelled oligonucleotide exon probe cocktails.
Howie
S E
SE
Department of Dermatology, University of Edinburgh, Scotland.
Aldridge
R D
RD
McVittie
E
E
Thornton
E
E
Ramage
E
E
Hunter
J A
JA
D000328:Adult; D001706:Biopsy; D016207:Cytokines / Q000235:genetics*; D003877:Dermatitis, Contact / Q000378:metabolism / Q000473:pathology; D004817:Epidermis / Q000378:metabolism* / Q000473:pathology; D005260:Female; D016650:Fluorescein-5-isothiocyanate; D006801:Humans; D017403:In Situ Hybridization / Q000379:methods*; D008297:Male; D008875:Middle Aged; D009532:Nickel / Q000009:adverse effects; D015345:Oligonucleotide Probes; D012333:RNA, Messenger / Q000378:metabolism*; D012867:Skin / Q000378:metabolism / Q000473:pathology
D016207:Cytokines; D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D009532:Nickel; D016650:Fluorescein-5-isothiocyanate