Expression of the CD4 gene requires a Myb transcription factor.
We have analyzed the control of developmental expression of the CD4 gene, which encodes an important recognition molecule and differentiation antigen on T cells. We have determined that the CD4 promoter alone functions at high levels in the CD4+ CD8- mature T cell but not at the early CD4+ CD8+ stage of T-cell development. In addition, the CD4 promoter functions only in T lymphocytes; thus, the stage and tissue specificity of the CD4 gene is mediated in part by its promoter. We have determined that a Myb transcription factor binds to the CD4 promoter and is critical for full promoter function. Thus, Myb plays an important role in the expression of T-cell-specific developmentally regulated genes.
Siu
G
G
Department of Biology, University of California, San Diego, La Jolla 92093-0063.
Wurster
A L
AL
Lipsick
J S
JS
Hedrick
S M
SM
D000818:Animals; D001483:Base Sequence; D015704:CD4 Antigens / Q000235:genetics*; D015496:CD4-Positive T-Lymphocytes / Q000378:metabolism*; D002454:Cell Differentiation; D002460:Cell Line; D004252:DNA Mutational Analysis; D005786:Gene Expression Regulation; D051379:Mice; D008969:Molecular Sequence Data; D009857:Oncogenes / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics*; D014018:Tissue Distribution; D014157:Transcription Factors / Q000235:genetics*; D014158:Transcription, Genetic; D015533:Transcriptional Activation
D015704:CD4 Antigens; D014157:Transcription Factors