Identification of 57 conventional RFLP and 6 VNTR systems with 32 DNA clones on chromosome 11p15.
Fifty-four clones containing human inserts were selected from a cosmid library constructed from a somatic cell hybrid containing chromosome 11p15.3-p15.5 as its only human complement. In 32 of these clones, 63 polymorphic systems were identified with a panel of restriction enzymes: 57 conventional RFLP systems and 6 highly polymorphic VNTR systems. Although we examined the cosmid with only seven enzymes, 18 clones (including 6 VNTRs) were polymorphic with three or more enzymes. The results suggested that DNA sequences on the peritelomeric region of chromosome 11p tend to be highly variable. Because these markers are highly informative, they will be excellent resources for investigations of hereditary diseases and tumor suppressor genes in this region of chromosome 11.
Department of Biochemistry, Cancer Institute, Tokyo, Japan.
K
K
Takita
A
A
Tanigami
T
T
Tokino
C
C
Jones
Y
Y
Nakamura
D000818:Animals; D001345:Autoradiography; D015139:Blotting, Southern; D002874:Chromosome Mapping; D002880:Chromosomes, Human, Pair 11; D003001:Cloning, Molecular; D006224:Cricetinae; D004247:DNA; D005819:Genetic Markers; D006801:Humans; D006822:Hybrid Cells; D012150:Polymorphism, Restriction Fragment Length; D012091:Repetitive Sequences, Nucleic Acid
D005819:Genetic Markers; D004247:DNA