Thioguanine-resistant mutant frequency in T-lymphocytes from a healthy human population.
Resistance to 6-thioguanine in T-lymphocytes was used to study in vivo somatic mutations in normal healthy adults. Donor age had a significant effect on mutant frequency at the hprt locus, showing an increase of 0.09/10(6) cells per year of age. No significant increase was associated with sex of donor, smoking habits, alcohol or coffee/tea intake, or X-ray exposure. The lower mutant frequency seen with contraceptive pill usage was probably due to the age difference between the groups of users and non-users.
BIBRA Toxicology International, Carshalton, Surrey, Great Britain.
M J
MJ
Davies
D P
DP
Lovell
D
D
Anderson
D000328:Adult; D000375:Aging / Q000235:genetics; D000704:Analysis of Variance; D002999:Clone Cells; D003276:Contraceptives, Oral / Q000494:pharmacology; D004351:Drug Resistance / Q000235:genetics; D005260:Female; D006801:Humans; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009154:Mutation; D012016:Reference Values; D012044:Regression Analysis; D012907:Smoking; D013601:T-Lymphocytes; D013866:Thioguanine / Q000494:pharmacology*
D003276:Contraceptives, Oral; D007041:Hypoxanthine Phosphoribosyltransferase; D013866:Thioguanine