Identification of human blood in mosquitoes (Diptera: Culicidae) using nonradioactive DNA dot blot hybridization.
A dot blot hybridization procedure was developed to detect human blood meals in engorged mosquitoes. A biotinylated DNA probe allowed the detection of 10-100 ng of human DNA, discriminated well between human and nonhuman sources of blood, and cross-reacted only with monkey DNA. Results showed that this method was a specific and sensitive technique for the identification of blood meals up to 100 h after ingestion. The nonisotopic label offers easy handling without the problems inherent in the use of radioisotopes, and it can be adapted for use in routine field tests.
Department of Biology, Kagawa Medical School, Japan.
C
C
Sato
Y
Y
Furuya
M
M
Harada
S
S
Suguri
D000818:Animals; D009033:Culicidae; D004247:DNA / Q000097:blood* / Q000235:genetics; D015342:DNA Probes; D005914:Globins / Q000235:genetics; D006801:Humans; D007303:Insect Vectors; D009693:Nucleic Acid Hybridization; D013045:Species Specificity
D015342:DNA Probes; D005914:Globins; D004247:DNA