The protective effect of serum against hydrodynamic damage of hybridoma cells in agitated and surface-aerated bioreactors.
The effect of serum on the growth rate and metabolism of CRL-8018 hybridoma cells in an agitated, surface-aerated bioreactor was examined. In the employed well-controlled bioreactors at high agitation rates, hybridoma cells in medium containing 1% fetal bovine serum rapidly die in the presence of a vortex with accompanying gas-bubble entrainment, whereas non-agitated control cultures grow normally in medium containing 1% serum. Serum levels greater than 5% counteract the detrimental hydrodynamic effects due to agitation and bubble entrainment. The protective effect is present after short-term (less than 1 h) exposure to 10% serum concentration, suggesting a protection mechanism which is, at least in part, of a physical nature. The apparent cell yields on glucose, lactate, and glutamine decreased with decreasing growth rate due to low serum concentrations. The results are incorporated into a simple model in which the apparent growth rate is the sum of an invariable growth rate and a changing death rate.
Department of Chemical Engineering, Rice University, Houston, Texas.
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Papoutsakis
D000818:Animals; D000941:Antigens / Q000276:immunology; D001769:Blood; D002455:Cell Division; D002470:Cell Survival; D002478:Cells, Cultured; D003470:Culture Media; D003584:Cytological Techniques; D005947:Glucose / Q000378:metabolism; D005973:Glutamine / Q000378:metabolism; D006514:Hepatitis B Surface Antigens / Q000276:immunology; D006825:Hybridomas / Q000166:cytology* / Q000378:metabolism; D007700:Kinetics; D007773:Lactates / Q000378:metabolism; D019344:Lactic Acid; D051379:Mice; D055585:Physical Phenomena; D010825:Physics
D000941:Antigens; D003470:Culture Media; D006514:Hepatitis B Surface Antigens; D007773:Lactates; D005973:Glutamine; D019344:Lactic Acid; D005947:Glucose