Electrotransformation of intact cells of Brevibacterium flavum MJ-233.
Electroporation allowed transformation of intact cells of Brevibacterium flavum MJ-233. The two plasmids used for electroporation were pCRY2 (6.3 kilobase) and pCRY3 (8.2 kilobases). Both plasmids contain the chloramphenicol-resistance gene and the autonomous replication origin in B. flavum MJ-233. The efficiency of electrotransformation was optimal with cells harvested at the middle log phase of growth, and was improved by the addition of 1.0 U/ml of penicillin G to the culture medium. The optimum yield of transformants per micrograms DNA was 5 x 10(4) when the cell suspension was pulsed at a cell density of 1 x 10(10)/ml and at a DNA amount of 1.0 micrograms.
Tsukuba Research Center, Mitsubishi Petrochemical Co. Ltd., Ibaraki, Japan.
Y
Y
Satoh
K
K
Hatakeyama
K
K
Kohama
M
M
Kobayashi
Y
Y
Kurusu
H
H
Yukawa
D001951:Brevibacterium / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics* / Q000378:metabolism; D005822:Genetic Vectors; D009997:Osmotic Pressure; D010400:Penicillin G / Q000494:pharmacology; D010957:Plasmids; D011523:Protoplasts / Q000502:physiology; D014169:Transformation, Bacterial
D004269:DNA, Bacterial; D010400:Penicillin G