A chromosome 9 deletion in Plasmodium falciparum results in loss of cytoadherence.
Many lines of Plasmodium falciparum undergo a deletion of the right end of chromosome 9 during in vitro culture accompanied by loss of cytoadherence and gametocytogenesis. Selection of cytoadherent cells from a mixed population co-selects for those with an undeleted chromosome 9 and the selected cells produce gametocytes. The deletion also results in loss of expression of PfEMP1, the putative cytoadherence ligand, suggesting that PfEMP1 or a regulatory gene controlling PfEMP1 expression and gametocytogenesis may be encoded in this region. We have isolated several markers for the deleted region and are currently using a YAC-P. falciparum library to investigate this region of the genome in detail.
Walter and Eliza Hall Institute of Medical Research, Royal Melbourne Hospital, Victoria, Australia.
D J
DJ
Kemp
J
J
Thompson
D A
DA
Barnes
T
T
Triglia
F
F
Karamalis
C
C
Petersen
G V
GV
Brown
K P
KP
Day
D000818:Animals; D001798:Blood Proteins / Q000235:genetics* / Q000502:physiology; D002448:Cell Adhesion / Q000235:genetics; D002872:Chromosome Deletion; D018244:Chromosomes, Artificial, Yeast; D016521:Electrophoresis, Gel, Pulsed-Field; D017125:Genes, Protozoan; D010274:Parasitology / Q000379:methods; D010455:Peptides / Q000235:genetics / Q000502:physiology; D010963:Plasmodium falciparum / Q000235:genetics* / Q000502:physiology; D015800:Protozoan Proteins; D012098:Reproduction; D012641:Selection, Genetic; D014407:Tumor Cells, Cultured
D001798:Blood Proteins; D010455:Peptides; D015800:Protozoan Proteins; C085731:erythrocyte membrane protein 1, Plasmodium falciparum; C042968:knob protein, Plasmodium falciparum