Detection of bovine alpha s1-casein genomic variants using the allele-specific polymerase chain reaction.
A method was developed to distinguish between genotypic variants B and C of bovine alpha s1-casein, using the allele-specific polymerase chain reaction (ASPCR). The alpha s1-casein genotype determined for 17 Jersey cows by the ASPCR method was confirmed by typing the alpha s1-casein milk proteins on isoelectric focusing gels. Using the ASPCR method described, rapid analysis of the alpha s1-casein genotype of bulls is now possible. In addition, kappa-casein genotypes can be determined from the same PCR reaction.
David
V A
VA
BioMolecular Research Department, W. R. Grace & Co.-Conn., Columbia, Maryland 21044.
Deutch
A H
AH
D000483:Alleles; D000818:Animals; D001483:Base Sequence; D002364:Caseins / Q000235:genetics*; D002417:Cattle; D004277:DNA, Single-Stranded; D005260:Female; D014644:Genetic Variation; D005838:Genotype; D005971:Glutamates / Q000235:genetics; D018698:Glutamic Acid; D005998:Glycine / Q000235:genetics; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction
D002364:Caseins; D004277:DNA, Single-Stranded; D005971:Glutamates; D018698:Glutamic Acid; D005998:Glycine