Isolation, primary structure and synthesis of neomyosuppressin, a myoinhibiting neuropeptide from the grey fleshfly, Neobellieria bullata.
1. An amidated decapeptide, showing strong inhibitory activity of spontaneous visceral muscle movement was isolated, from head extracts of 42 thousand fleshflies, Neobellieria bullata (Diptera, Sarcophagidae). 2. Amino acid sequencing and verification by peptide synthesis revealed the following primary structure: Thr-Asp-Val-Asp-His-Val-Phe-Leu-Arg-PheNH2. 3. The novel peptide was termed neomyosuppressin or Neb-MS. 4. During the process of consecutive high performance liquid chromatography (HPLC) purifications the biological activity of the samples was monitored using heterologous bioassay system. 5. The threshold level of synthetic Neb-MS was found to be 8.6 +/- 0.5 x 10(-11) M on the Leucophaea hindgut and 3.4 +/- 0.5 x 10(-10) M on the Locusta oviduct bioassay, respectively.
Fónagy
A
A
Plant Protection Institute of the Hungarian Academy of Sciences, Budapest II.
Schoofs
L
L
Proost
P
P
Van Damme
J
J
Bueds
H
H
De Loof
A
A
D000595:Amino Acid Sequence; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004175:Diptera / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D005260:Female; D006110:Grasshoppers / Q000187:drug effects; D007301:Insect Hormones / Q000138:chemical synthesis / Q000302:isolation & purification* / Q000494:pharmacology; D019476:Insect Proteins; D008297:Male; D008969:Molecular Sequence Data; D009119:Muscle Contraction / Q000187:drug effects*; D009479:Neuropeptides / Q000138:chemical synthesis / Q000302:isolation & purification* / Q000494:pharmacology
D007301:Insect Hormones; D019476:Insect Proteins; D009479:Neuropeptides; C073324:locustamyoinhibiting peptide, Locusta migratoria; C077905:neomyosuppressin