Action pattern of Streptomyces griseus chitinase on partially N-acetylated chitosan.
Oligosaccharides produced during the course of the hydrolysis of 25% N-acetylated chitosan by Streptomyces griseus chitinase were fractionated by CM-Sephadex C-25 and Toyopearl HW-40F column chromatographies. Sugar compositions and sequences of main oligosaccharides were identified by N-acetylation, exo-splitting with beta-GlcNAcase and beta-GlcNase, and nitrous acid degradation. In addition to N-acetylated saccharides, GlcNAc, (GlcNAc)2, and (GlcNAc)3, hetero-chitooligosaccharides such as GlcN.GlcNAc, GlcN.GlcNAc.GlcNAc, GlcN.GlcN.GlcNAc, GlcN.GlcNAc.GlcNAc.GlcNAc, GlcNAc.GlcN.GlcNAc.GlcNAc, GlcN.GlcNAc.GlcN.GlcNAc, and GlcN.GlcN.GlcNAc.GlcNAc were identified. These results indicate that Streptomyces griseus chitinase specifically cleaves the N-acetyl-beta-D-glucosaminidic linkages in partially N-acetylated chitosan.
Department of Applied Biological Sciences, Faculty of Agriculture, Saga University, Japan.
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Ohtakara
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Matsunaga
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Mitsutomi
D000107:Acetylation; D002240:Carbohydrate Sequence; D002686:Chitin / Q000031:analogs & derivatives* / Q000378:metabolism; D002688:Chitinases / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D048271:Chitosan; D002851:Chromatography, High Pressure Liquid; D006868:Hydrolysis; D008969:Molecular Sequence Data; D009608:Nitrous Acid; D009844:Oligosaccharides / Q000032:analysis / Q000302:isolation & purification / Q000378:metabolism; D013305:Streptomyces griseus / Q000201:enzymology* / Q000235:genetics
D009844:Oligosaccharides; D002686:Chitin; D048271:Chitosan; D002688:Chitinases; D009608:Nitrous Acid