Hepatocyte growth factor is a paracrine factor for renal epithelial cells: stimulation of DNA synthesis and NA,K-ATPase activity.
The expressions of mRNAs of hepatocyte growth factor (HGF) and its receptor (c-met) and its effects were examined in cultured renal epithelial cell lines (OK, LLCPK1, and MDCK cells) and rat mesangial cells in primary culture. Northern blot analysis revealed the presence of HGF mRNA in mesangial cells, but not in epithelial cells. c-met mRNA was detected in epithelial cells, but not in mesangial cells. HGF stimulated [3H]-thymidine incorporation (DNA synthesis) dose-dependently in OK and LLCPK1 cells, but not in MDCK and mesangial cells. Ouabaine sensitive rubidium uptake (Na,K-ATPase activity) was stimulated by 63% with HGF (10 ng/ml) treatment for 16hr in MDCK cells. The results suggest that HGF is produced in the kidney, at least in mesangial cells and works on epithelial cells to stimulate the proliferation and/or to modify cell functions in a paracrine manner.
Second Department of Internal Medicine, Tokyo Medical and Dental University, Japan.
K
K
Ishibashi
S
S
Sasaki
H
H
Sakamoto
Y
Y
Nakamura
T
T
Hata
T
T
Nakamura
F
F
Marumo
D000818:Animals; D015152:Blotting, Northern; D002460:Cell Line; D004247:DNA / Q000096:biosynthesis; D004261:DNA Replication / Q000187:drug effects; D004815:Epidermal Growth Factor / Q000494:pharmacology; D004848:Epithelium / Q000502:physiology; D005920:Glomerular Mesangium; D006133:Growth Substances / Q000235:genetics / Q000494:pharmacology / Q000502:physiology*; D017228:Hepatocyte Growth Factor; D007668:Kidney; D007700:Kinetics; D010042:Ouabain / Q000494:pharmacology; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000502:physiology; D019859:Proto-Oncogene Proteins c-met; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D011956:Receptors, Cell Surface / Q000502:physiology; D012413:Rubidium / Q000378:metabolism; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*; D013936:Thymidine / Q000378:metabolism
D006133:Growth Substances; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D010042:Ouabain; D004815:Epidermal Growth Factor; D012313:RNA; D017228:Hepatocyte Growth Factor; D004247:DNA; D019859:Proto-Oncogene Proteins c-met; D000254:Sodium-Potassium-Exchanging ATPase; D012413:Rubidium; D013936:Thymidine