The molecular analysis of synonymy among medically important yeasts within the genus Candida.
Three sets of medically important yeasts, Candida albicans, C. tropicalis, and C. krusei, were compared with their putative synonyms (C. langeronii and C. claussenii, C. paratropicalis, and Itssatchenkia orientalis, respectively) to determine if these synonyms are genetically distinguishable from each other. Pulsed-field electrophoresis and hybridization to species-specific probes were used to accomplish this goal. The species-specific probes for C. albicans and C. tropicalis have been previously described (27A and CT13.8, respectively) whereas the probe for C. krusei (CK3) was cloned in this study. No distinguishing characteristics between synonyms were identified, thus supporting the current taxonomic treatment of these organisms.
Laboratory of Clinical Investigation, National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892.
B L
BL
Wickes
J B
JB
Hicks
W G
WG
Merz
K J
KJ
Kwon-Chung
D002175:Candida / Q000145:classification* / Q000235:genetics; D002176:Candida albicans / Q000145:classification / Q000235:genetics; D015342:DNA Probes; D015246:Deoxyribonuclease EcoRI / Q000378:metabolism; D016521:Electrophoresis, Gel, Pulsed-Field; D007621:Karyotyping; D015336:Molecular Probe Techniques; D016533:Mycological Typing Techniques; D009693:Nucleic Acid Hybridization; D012150:Polymorphism, Restriction Fragment Length; D012689:Sequence Homology, Nucleic Acid
D015342:DNA Probes; D015246:Deoxyribonuclease EcoRI