DNA fingerprinting--a valuable new technique for the characterisation of cell lines.
DNA fingerprinting is an important new development for the authentication of cell lines. Multilocus methods such as those developed by Alec Jeffreys provide information on a wide range of genetic loci throughout the human genome and thus give a useful genetic "snap-shot" of a cell culture. Our work has shown that Jeffreys multilocus fingerprinting method can be applied to cell lines from a wide range of animals including reptiles, birds, fish and diverse mammals. It can also differentiate very closely related cell lines including those from the same mouse strain. Routine fingerprint analysis has enabled an unprecedented level of confidence in the consistency of cell stocks. Our results demonstrate that this straightforward method represents a powerful and readily interpreted system for cell authentication and exclusion of cross-contamination.
European Collection of Animal Cell Cultures and Animal Cell Technology, Biologics Division, PHLS-CAMR, Porton Down, Wiltshire, UK.
G
G
Stacey
B
B
Bolton
A
A
Doyle
B
B
Griffiths
D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D002460:Cell Line; D002874:Chromosome Mapping; D016172:DNA Fingerprinting; D006801:Humans; D015203:Reproducibility of Results