Perturbation of beta 1 integrin-mediated adhesions results in altered somite cell shape and behavior.
Cell contact and adhesion between somites and the axial extracellular matrix (ECM) is likely to play a fundamental role in vertebrate development. In a preliminary report we showed that injection of the monoclonal antibody CSAT, which recognizes the avian beta 1 integrins, causes a lateral separation of both somites and segmental plate tissue from the embryonic axis (Drake and Little, 1991). In this study we addressed the cell biological response to CSAT injection, particularly the cell-ECM interactions involved in maintaining normal somite-axial relationships. A total of 150 stage 7-10 quail embryos have been injected with CSAT and then cultured for varying periods (1-30 hr). CSAT caused somitic cells to behave abnormally. Changes include, rounding-up, extensive blebbing, and formation of retraction fibers. A majority of separated somites were able to assume normal axial position with further time culture. Whether a somite subsequently aligned at the axis was dependent on the amount of CSAT injected and the postinjection culture period. Embryos in which somites remained separated from the axis after relatively long culture intervals (18-24 hr) displayed abnormal sclerotomal cell migrations. In no case did control injected embryos exhibit cellular alterations. Similarly, the injection of RGD-containing peptides had no detectable effect on somitogenesis or somite/segmental plate adhesion to the axis. On the basis of these data, we conclude that beta 1 integrins are necessary for normal somitic cell adhesions to the axis, but not somite segmentation and differentiation.
Department of Anatomy, University of Virginia, Charlottesville 22908.
C J
CJ
Drake
L A
LA
Davis
J E
JE
Hungerford
C D
CD
Little
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D002448:Cell Adhesion / Q000502:physiology*; D002490:Central Nervous System / Q000196:embryology* / Q000648:ultrastructure; D003370:Coturnix / Q000196:embryology; D004305:Dose-Response Relationship, Drug; D005109:Extracellular Matrix / Q000378:metabolism*; D019012:Integrin beta1; D016023:Integrins / Q000276:immunology / Q000378:metabolism*; D008648:Mesoderm / Q000502:physiology* / Q000648:ultrastructure; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D009672:Notochord / Q000196:embryology* / Q000648:ultrastructure
D000911:Antibodies, Monoclonal; D019012:Integrin beta1; D016023:Integrins