The Rhizobium leguminosarum biovar phaseoli glnT gene, encoding glutamine synthetase III.
Plasmid pGE203 contains the Rhizobium leguminosarum biovar phaseoli glnT locus. Glutamine synthetase III (GSIII) was purified from a glutamine auxotrophic strain of Klebsiella pneumoniae carrying this plasmid. Sequencing of a 2.4-kb fragment containing the glnT locus reveals an open reading frame of 435 amino acids (aa), whose first eight aa are identical to those determined from pure GSIII by direct aa sequencing, thus confirming that glnT indeed codes for GSIII activity. The comparison of the GSIII aa sequence with the reported sequence of GSs from other organisms shows a significant degree of homology. Since the three-dimensional structure of GS from Salmonella typhimurium is known, a three-dimensional model of GSIII was built by homology.
Istituto Internazionale di Genetica e Biofisica, CNR, Napoli, Italy.
M
M
Chiurazzi
R
R
Meza
M
M
Lara
A
A
Lahm
R
R
Defez
M
M
Iaccarino
G
G
Espín
D000595:Amino Acid Sequence; D001483:Base Sequence; D004269:DNA, Bacterial; D005798:Genes, Bacterial; D005974:Glutamate-Ammonia Ligase / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D015151:Immunoblotting; D007527:Isoenzymes / Q000235:genetics*; D008969:Molecular Sequence Data; D011487:Protein Conformation; D016961:Rhizobium leguminosarum / Q000201:enzymology / Q000235:genetics*; D016415:Sequence Alignment
D004269:DNA, Bacterial; D007527:Isoenzymes; D005974:Glutamate-Ammonia Ligase