Effect of diets rich in medium-chain and long-chain triglycerides on lipogenic-enzyme gene expression in liver and adipose tissue of the weaned rat.
The activity and mRNA concentrations of two lipogenic enzymes, fatty-acid synthase and acetyl-CoA carboxylase were measured in the liver and white adipose tissue of rats weaned to a carbohydrate-rich diet containing either long-chain or medium-chain fatty acids, and compared to those of rats weaned on a diet containing less than 1% (total energy) fat (high-carbohydrate diet). In the liver, the diet containing long-chain fatty acids inhibited the increase of both lipogenic-enzyme mRNA concentrations and activities seen at weaning on the high-carbohydrate diet but did not prevent the decrease in phosphoenolpyruvate carboxykinase mRNA and activity. In contrast, the diet containing medium-chain fatty acids induced a slower but finally similar increase in lipogenic-enzyme mRNA concentrations and activities. In adipose tissue, a similar trend was observed, although the inhibitory effect of the diet containing long-chain fatty acids was considerably less marked than in liver. It is concluded that medium-chain and long-chain fatty acids have not the same inhibitory potency of the gene expression of lipogenic enzymes, and that long-chain fatty acids have a more marked effect in the liver.
Centre de Recherches sur l'Endocrinologie Moléculaire et le Développement, Centre National de la Recherche Scientifique, Meudon-Bellevue, France.
F
F
Foufelle
D
D
Perdereau
B
B
Gouhot
P
P
Ferre
J
J
Girard
D000103:Acetyl-CoA Carboxylase / Q000235:genetics; D000273:Adipose Tissue / Q000201:enzymology*; D000818:Animals; D015152:Blotting, Northern; D004040:Dietary Carbohydrates / Q000008:administration & dosage; D004041:Dietary Fats / Q000494:pharmacology*; D064429:Fatty Acid Synthases / Q000235:genetics; D005260:Female; D015870:Gene Expression / Q000187:drug effects*; D005934:Glucagon / Q000097:blood; D007328:Insulin / Q000097:blood; D007700:Kinetics; D008055:Lipids / Q000096:biosynthesis*; D008099:Liver / Q000201:enzymology*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D014280:Triglycerides / Q000494:pharmacology*; D014886:Weaning
D004040:Dietary Carbohydrates; D004041:Dietary Fats; D007328:Insulin; D008055:Lipids; D012333:RNA, Messenger; D014280:Triglycerides; D005934:Glucagon; D064429:Fatty Acid Synthases; D000103:Acetyl-CoA Carboxylase