Identification of homeobox-containing genes expressed in hematopoietic blast cells.
Among putative candidates involved in commitment and differentiation of hematopoietic cells are homeoproteins, a large family of transcription factors playing a major role during development. Using a polymerase chain reaction (PCR)-derived protocol, we have investigated for Antennapedia-like homeobox-containing (HOX) gene expression in an enriched population of human hematopoietic progenitors. Nine members of HOX 1 and HOX 2 loci were isolated. Together with recent studies using established cell lines, this indicates a large representation of HOX genes in the hematopoietic compartment and suggests a participation of this class of nuclear proteins to early steps of hematopoiesis.
INSERM U217-Laboratoire d'Hématologie, Département de Biologie Moléculaire et Structurale, Centre d'Etudes Nucléaires, Grenoble, France.
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Vieille-Grosjean
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Roullot
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Courtois
D000595:Amino Acid Sequence; D001483:Base Sequence; D015870:Gene Expression; D005801:Genes, Homeobox; D006412:Hematopoietic Stem Cells / Q000502:physiology*; D006801:Humans; D066298:In Vitro Techniques; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics*; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics
D009838:Oligodeoxyribonucleotides; D012333:RNA, Messenger