Control of in vivo proteolysis in the production of recombinant proteins.
Proteolytic degradation of protein products causes many problems in the bioprocess industry. The increasing production of proteins in heterologous hosts through the use of recombinant DNA technology, has recently brought the problem into focus, since it seems that heterologous proteins are more prone to proteolysis. The result of proteolytic attack may vary from complete hydrolysis of the product to minor truncation of the protein without impairment of its biological function. The degree to which such partial proteolysis is a problem depends on the requirements of the ultimate use of the protein product.
Department of Biochemistry and Biotechnology, Royal Institute of Technology, Stockholm, Sweden.
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Enfors
D001709:Biotechnology; D004355:Drug Stability; D006360:Heat-Shock Proteins / Q000096:biosynthesis; D002479:Inclusion Bodies / Q000378:metabolism; D010447:Peptide Hydrolases / Q000378:metabolism; D015202:Protein Engineering; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics
D006360:Heat-Shock Proteins; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins; D010447:Peptide Hydrolases