Isolation and characterization of type 1 fimbriae from a chicken pathogenic Escherichia coli serotype O78.
Type 1 fimbriae from chicken pathogenic Escherichia coli strain PDI-386 (serotype O78) was purified and characterized. Because of the acid-induced autoagglutination (T. Sekizaki, Y. Nakasato, and I. Nonomura, J. Vet. Med. Sci. 54, 493-499, 1992), the fimbriae could be easily purified by repeating acid sedimentation, washing, and dissolving in buffer (pH 8.0). In electron microscopy, the purified fimbriae showed a filament of 8 nm in diameter and 10 microns in average length. The molecular mass of the protein subunit of the purified fimbriae estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) was 19,000 daltons. The amino acid composition and its NH2-terminal sequence were similar to the previously described one of the Klebsiella pneumoniae type 1 fimbriae. Moreover, there was an immunological relatedness between them. These results indicated that a molecular diversity found between the fimbriae of E. coli and that of K. pneumoniae has already been existed among chicken pathogenic E. coli strains.
National Institute of Animal Health, Ibaraki, Japan.
T
T
Sekizaki
S
S
Miyazaki
H
H
Ito
T
T
Asawa
I
I
Nonomura
D000595:Amino Acid Sequence; D000818:Animals; D002645:Chickens / Q000382:microbiology*; D004926:Escherichia coli / Q000737:chemistry / Q000302:isolation & purification / Q000648:ultrastructure*; D004927:Escherichia coli Infections / Q000382:microbiology / Q000662:veterinary; D010861:Fimbriae, Bacterial / Q000737:chemistry*; D008969:Molecular Sequence Data; D011201:Poultry Diseases / Q000382:microbiology*