The turkey major histocompatibility complex: identification of class II genotypes by restriction fragment length polymorphism analysis of deoxyribonucleic acid.
Using a chicken Class II MHC clone in Northern blot analysis, tissue-specific expression of turkey Class II MHC genes was observed in the embryonic bursa of Fabricius as well as in the adult spleen. In contrast, there was no detectable expression in the embryonic liver, brain, or spleen. Southern blot analysis of BamHI-digested turkey DNA revealed two restriction fragment length polymorphism (RFLP) patterns that did not deviate significantly from single-gene Mendelian inheritance. Further analysis of PvuII-digested DNA from 325 turkeys showed four distinct RFLP patterns that segregated within the turkey lines studied. Because the chicken Class II MHC clone hybridized specifically to mRNA in immune-associated tissues, and because it identified polymorphisms among turkeys, the chicken clone is suggested to identify four turkey Class II MHC genotypes. The current study provides good evidence that RFLP analysis of DNA can be used as a means for molecular genotyping at the MHC in turkeys.
Department of Poultry Science, Ohio State University, Wooster 44691.
M G
MG
Emara
K E
KE
Nestor
D N
DN
Foster
S J
SJ
Lamont
D000818:Animals; D015139:Blotting, Southern; D002060:Bursa of Fabricius / Q000737:chemistry / Q000196:embryology; D004247:DNA / Q000032:analysis* / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific; D005802:Genes, MHC Class II / Q000235:genetics*; D005838:Genotype; D009693:Nucleic Acid Hybridization; D012150:Polymorphism, Restriction Fragment Length; D012333:RNA, Messenger / Q000032:analysis; D013154:Spleen / Q000737:chemistry; D014018:Tissue Distribution; D014422:Turkeys / Q000235:genetics* / Q000276:immunology
D012333:RNA, Messenger; D004247:DNA; C025630:CAGCTG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific