Tryptophan inhibits the [3H]glutamate uptake into Xenopus oocytes injected with rat brain mRNA.
We characterized the glutamate (Glu) uptake in Xenopus oocytes injected with rat brain mRNA. The Glu uptake into oocytes was higher in mRNA-injected oocytes than in vehicle-injected ones. Na+ omission or addition of tryptophan inhibited the uptake in mRNA-injected oocytes, although it did not affect that in vehicle-injected oocytes. These results suggest that Glu transporters with a tryptophan sensitivity different from that of Glu transporters in native oocytes are expressed after injection of rat brain mRNA.
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.
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Tohda
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Urushihara
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Nomura
D027322:Amino Acid Transport System X-AG; D000818:Animals; D001921:Brain / Q000378:metabolism; D001923:Brain Chemistry; D005971:Glutamates / Q000493:pharmacokinetics*; D018698:Glutamic Acid; D006023:Glycoproteins / Q000378:metabolism; D008297:Male; D014179:Neurotransmitter Uptake Inhibitors / Q000494:pharmacology; D009865:Oocytes / Q000378:metabolism*; D012333:RNA, Messenger / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D012680:Sensitivity and Specificity; D014316:Tritium; D014364:Tryptophan / Q000494:pharmacology*; D014982:Xenopus laevis
D027322:Amino Acid Transport System X-AG; D005971:Glutamates; D006023:Glycoproteins; D014179:Neurotransmitter Uptake Inhibitors; D012333:RNA, Messenger; D014316:Tritium; D018698:Glutamic Acid; D014364:Tryptophan