Functional heterogeneity of type 1 fimbriae of Escherichia coli.
Escherichia coli and other members of the family Enterobacteriaceae express surface fibrillar structures, fimbriae, that promote bacterial adhesion to host receptors. Type 1 fimbriae possess a lectinlike component, FimH, that is commonly thought to cause binding to mannose-containing oligosaccharides of host receptors. Since adhesion of type 1 fimbriated organisms are inhibited by mannose, the reactions are described as mannose sensitive (MS). We have studied the adhesion of the type 1 fimbriated CSH-50 strain of E. coli (which expresses only type 1 fimbriae) to fibronectin (FN). E. coli CSH-50 does not bind detectable amounts of soluble FN but adheres well to immobilized plasma or cellular FN. This adhesion was inhibited by mannose-containing saccharides. By using purified domains of FN, it was found that E. coli CSH-50 adheres primarily to the amino-terminal and gelatin-binding domains, only one of which is glycosylated, in an MS fashion. Binding of the mannose-specific lectin concanavalin A to FN and ovalbumin was eliminated or reduced, respectively, by incubation with periodate or endoglycosidase. Adhesion of E. coli CSH-50 to ovalbumin was reduced by these treatments, but adhesion to FN was unaffected. E. coli CSH-50 also adheres to a synthetic peptide copying a portion of the amino-terminal FN domain (FNsp1) in an MS fashion. Purified CSH-50 fimbriae bound to immobilized FN and FNsp1 in an MS fashion and inhibited adhesion of intact organisms. However, fimbriae purified from HB101 (pPKL4), a recombinant strain harboring the entire type 1 fim gene locus and expressing functional type 1 fimbriae, neither bound to FN or FNsp1 nor inhibited E. coli adhesion to immobilized FN or FNsp1. These novel findings suggest that there are two forms of type 1 MS fimbriae. One form exhibits only the well-known MS lectinlike activity that requires a substratum of mannose-containing glycoproteins. The other form exhibits not only the MS lectinlike activity but also binds to nonglycosylated regions of proteins in an MS manner.
Department of Anatomy and Neurobiology, University of Tennessee, Memphis 38104.
E V
EV
Sokurenko
H S
HS
Courtney
S N
SN
Abraham
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P
Klemm
D L
DL
Hasty
D001422:Bacterial Adhesion; D001665:Binding Sites; D004926:Escherichia coli / Q000472:pathogenicity*; D005353:Fibronectins / Q000378:metabolism; D010861:Fimbriae, Bacterial / Q000378:metabolism*; D006388:Hemagglutinins / Q000378:metabolism; D006801:Humans; D010084:Oxidation-Reduction; D010446:Peptide Fragments / Q000378:metabolism; D010504:Periodic Acid / Q000737:chemistry; D013211:Staphylococcus aureus / Q000472:pathogenicity
D005353:Fibronectins; D006388:Hemagglutinins; D010446:Peptide Fragments; D010504:Periodic Acid