Morphological changes of cultured rat hepatocytes exposed to methylglyoxal. Calcium-independence of injury.
Methylglyoxal-induced morphological changes were studied in cultured rat hepatocytes. Hepatocytes incubated either in the presence or absence of 2.5 mM Ca2+ were injured by 10 mM methylglyoxal to a similar extent, while 1 mM of the alpha-oxoaldehyde caused a moderate damage only in the absence of Ca2+. In the absence of Ca2+, however, hepatocytes were already injured, even without the presence of methylglyoxal hepatotoxicity proceeds independently of the Ca2+ influx.
Semmelweis University Medical School, First Institute of Biochemistry, Budapest, Hungary.
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Kalapos
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de Groot
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D008099:Liver / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D011765:Pyruvaldehyde / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar
D011765:Pyruvaldehyde; D002118:Calcium