Effect of antiserum to rat adipocytes on growth and body composition of the rat.
1. Antibody against intact adipocytes was produced in sheep and was capable of binding to intact rat adipocytes using an immunofluorescent assay. An antibody dose level was established and subsequently used in vivo. 2. This study demonstrated inconsistent response of passive immunization procedures with crude antibody against rat adipocytes. Establishment of specific protocols for each antibody preparation would be essential for using this immunological approach in body fat reduction.
Department of Animal Sciences, Oregon State University, Corvallis 97331-6702.
C Y
CY
Hu
A
A
Suryawan
J
J
Killefer
N B
NB
Wehr
D000273:Adipose Tissue / Q000166:cytology / Q000276:immunology*; D000818:Animals; D001823:Body Composition; D005455:Fluorescent Antibody Technique; D007106:Immune Sera; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains / Q000254:growth & development*
D007106:Immune Sera