Isolation and characterization of a lectin from the seeds of Dioclea lehmanni.
Affinity chromatography of the globulin fraction from the seeds of Dioclea lehmanni on Sephacryl S-200 yielded two lectins, one slightly retarded and another strongly bound. The latter, which was a glucose/mannose specific lectin, was purified and the following properties were determined: pI, Mr of subunits, carbohydrate content, A, aminoacid composition, hemagglutination and inhibition patterns, N-terminal sequence and mitogenic activity. These properties of the lectin were very similar to those of the Con A and Dioclea grandiflora lectins.
Perez
G
G
Department of Chemistry Universidad Nacional, Bogota, Colombia.
Hernandez
M
M
Mora
E
E
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002241:Carbohydrates; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D006384:Hemagglutination; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D007202:Indicators and Reagents; D037102:Lectins / Q000737:chemistry / Q000302:isolation & purification*; D037121:Plant Lectins; D012639:Seeds / Q000032:analysis*
D000596:Amino Acids; D002241:Carbohydrates; D007202:Indicators and Reagents; D037102:Lectins; D037121:Plant Lectins