Effects of pantethine on lipogenesis and CO2 production in the isolated hepatocytes of the chick (Gallus domesticus).
1. Isolated hepatocytes from chicks were used to study the effects of pantethine supplementation to incubation medium on in vitro lipogenesis, CO2 production and beta-oxidation of fatty acid. 2. In vitro lipogenesis, determined by the incorporation of 1-[14C]acetate into total lipid and various lipid fractions, as depressed in concordance with the increase of pantethine concentration in the medium. 3. Incubation of isolated hepatocytes with pantethine resulted in a significant decrease (P < 0.01) in the activities of acetyl-CoA carboxylase and fatty acid synthetase. 4. The results suggest that in vitro fatty acid synthesis from 1-[14C]acetate was depressed and CO2 production was elevated in hepatocytes of chicks through pantethine addition to the medium at a low level.
Department of Animal Science and Technology, Faculty of Agriculture, Gifu University, Japan.
J C
JC
Hsu
K
K
Tanaka
I
I
Inayama
S
S
Ohtani
D000818:Animals; D001835:Body Weight; D002245:Carbon Dioxide / Q000378:metabolism*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002645:Chickens / Q000378:metabolism*; D005227:Fatty Acids / Q000378:metabolism; D008055:Lipids / Q000096:biosynthesis*; D008099:Liver / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D009929:Organ Size; D010084:Oxidation-Reduction; D010204:Pantetheine / Q000031:analogs & derivatives* / Q000494:pharmacology
D005227:Fatty Acids; D008055:Lipids; D002245:Carbon Dioxide; D010204:Pantetheine; C005425:pantethine