Elimination of by-products in 11 beta-hydroxylation of substance S using Curvularia lunata clones regenerated from NTG-treated protoplasts.
Stable mutants showing improved 11-hydroxylation of Substance S were isolated, following treatment with N-methyl-N'-nitro-N-nitrosoguanidine (NTG) and regeneration of uninucleate protoplasts of the appropriate fungal strains. This procedure was especially suitable for obtaining more directed 11 beta-hydroxylation of Substance S with Curvularia lunata IM 2901. Apart from producing cortisol (11 beta-hydroxy-S), the parent strain formed several by-products that significantly lowered the yield of the desired 11 beta-hydroxyderivative. Isolated mutants of this microorganism carried out directed 11 beta-hydroxylation with only a small amount of one of the by-products, which resulted in a much higher yield of cortisol.
Wilmańska
D
D
Institute of Microbiology, University of Lódź, Poland.
Milczarek
K
K
Rumijowska
A
A
Bartnicka
K
K
Sedlaczek
L
L
D001711:Biotransformation; D003350:Cortodoxone / Q000378:metabolism*; D006854:Hydrocortisone / Q000096:biosynthesis; D006900:Hydroxylation; D008769:Methylnitronitrosoguanidine / Q000494:pharmacology; D003904:Mitosporic Fungi / Q000378:metabolism; D009090:Mucorales / Q000378:metabolism; D011523:Protoplasts / Q000187:drug effects / Q000378:metabolism*
D008769:Methylnitronitrosoguanidine; D003350:Cortodoxone; D006854:Hydrocortisone