Cloning of murine and rat vascular cell adhesion molecule-1.
Vascular cell adhesion molecule-1 (VCAM1) is a member of the immunoglobulin (Ig) superfamily which interacts with the integrin very late antigen 4 (VLA4). We have cloned the cDNAs for both murine and rat VCAM1 from endotoxin-treated lung libraries. Both sequences encode proteins with seven extracellular Ig-like domains, which show 75.9% and 76.9% identity, respectively, with human VCAM1. Both murine and human cell lines show VLA4-dependent binding to COS cells transiently expressing murine and rat VCAM1. Two mAbs, M-K/1 and M-K/2, which recognize an antigen on murine bone marrow stromal cell lines, bind to murine VCAM1 expressed in COS cells and block VCAM1-dependent adhesion, confirming that these mAbs recognize murine VCAM1.
Biogen, Inc., 14 Cambridge Center, MA 02142.
C
C
Hession
P
P
Moy
R
R
Tizard
P
P
Chisholm
C
C
Williams
M
M
Wysk
L
L
Burkly
K
K
Miyake
P
P
Kincade
R
R
Lobb
D000595:Amino Acid Sequence; D000818:Animals; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000235:genetics*; D002460:Cell Line; D003001:Cloning, Molecular; D005786:Gene Expression Regulation; D015723:Gene Library; D007249:Inflammation / Q000378:metabolism; D008168:Lung / Q000502:physiology; D051379:Mice; D008969:Molecular Sequence Data; D051381:Rats; D012689:Sequence Homology, Nucleic Acid; D014162:Transfection; D019010:Vascular Cell Adhesion Molecule-1
D015815:Cell Adhesion Molecules; D019010:Vascular Cell Adhesion Molecule-1