In vivo identification and monitoring of changes in rat brain glucose by two-dimensional shift-correlated 1H NMR spectroscopy.
Intracerebral glucose resonance was directly detected and resolved in vivo by two-dimensional shift-correlated (COSY) 1H NMR spectroscopy in anesthetized rats (n = 4). The relative changes in brain glucose concentration were measured by volume integration of the alpha-D-glucose cross peak in the 2D COSY spectra. This report demonstrates the possibility of monitoring the variations in cerebral glucose following iv injection of glucose.
Institut de Chimie des Substances Naturelles C.N.R.S., Gif sur Yvette, France.
M
M
Peres
O
O
Fedeli
B
B
Barrere
B
B
Gillet
G
G
Berenger
J
J
Seylaz
J C
JC
Beloeil
D000818:Animals; D001224:Aspartic Acid / Q000031:analogs & derivatives / Q000378:metabolism; D001786:Blood Glucose / Q000032:analysis; D001921:Brain / Q000378:metabolism*; D005947:Glucose / Q000008:administration & dosage / Q000378:metabolism*; D005971:Glutamates / Q000378:metabolism; D018698:Glutamic Acid; D005973:Glutamine / Q000378:metabolism; D006859:Hydrogen; D007294:Inositol / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008297:Male; D008991:Monitoring, Physiologic; D051381:Rats; D017208:Rats, Wistar
D001786:Blood Glucose; D005971:Glutamates; D005973:Glutamine; D001224:Aspartic Acid; D018698:Glutamic Acid; D007294:Inositol; D006859:Hydrogen; C000179:N-acetylaspartate; D005947:Glucose