Clonal differences within M-types of the group A Streptococcus revealed by pulsed field gel electrophoresis.
Digestion of chromosomal DNA with the rare cutting restriction enzyme SfiI in association with pulsed field gel electrophoresis was used to observe restriction fragment length polymorphisms (RFLP) among isolates of group A Streptococcus. Streptococci examined included isolates belonging to the same M-type (epidemiologically related and unrelated), and isolates from other M-types. RFLP patterns were quite distinct between all serotypes tested. More importantly, isolates from within a serotype could be differentiated by this technique.
New Zealand Communicable Disease Centre, Porirua.
L A
LA
Single
D R
DR
Martin
D004269:DNA, Bacterial / Q000737:chemistry; D016521:Electrophoresis, Gel, Pulsed-Field; D014644:Genetic Variation; D012150:Polymorphism, Restriction Fragment Length; D012703:Serotyping; D013297:Streptococcus pyogenes / Q000235:genetics*
D004269:DNA, Bacterial