Evidence that more than one gene encodes n-alkane-inducible cytochrome P-450s in Candida maltosa, found by two-step gene disruption.
An n-alkane-assimilating yeast, Candida maltosa, has a diploid genome. Probed with the previously isolated gene of n-alkane-inducible cytochrome P-450 (P-450alk), its allelic gene had been isolated, its nucleotides sequenced, and the interallelic divergence examined. Using one of the allelic genes, we disrupted the two alleles of the cytochrome P-450alk gene by a two-step gene disruption system. Surprisingly, the disruptant still assimilated n-alkane and contained n-alkane-inducible cytochrome P-450. This result indicates that, other than the disrupted two alleles, there is at least one other gene that encodes an n-alkane-inducible cytochrome P-450.
Department of Agricultural Chemistry, University of Tokyo, Japan.
M
M
Ohkuma
T
T
Hikiji
T
T
Tanimoto
W H
WH
Schunck
H G
HG
Müller
K
K
Yano
M
M
Takagi
D000473:Alkanes / Q000494:pharmacology; D000483:Alleles; D000595:Amino Acid Sequence; D001483:Base Sequence; D002175:Candida / Q000201:enzymology* / Q000235:genetics; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis / Q000235:genetics*; D004790:Enzyme Induction; D005656:Fungal Proteins / Q000096:biosynthesis / Q000235:genetics*; D008969:Molecular Sequence Data; D016296:Mutagenesis / Q000235:genetics
D000473:Alkanes; D005656:Fungal Proteins; D003577:Cytochrome P-450 Enzyme System