Epitope mapping of monoclonal antibodies to Torpedo acetylcholine receptor gamma subunits, which specifically recognize the epsilon subunit of mammalian muscle acetylcholine receptor.
Epitopes for four monoclonal antibodies (mAbs) to the gamma subunit of Torpedo nicotinic acetylcholine receptor (AChR), and one mAb crossreactive with the gamma and delta subunits of Torpedo AChR were mapped using overlapping synthetic peptides corresponding to the complete amino acid sequence of Torpedo gamma subunit. The epitopes for all mAbs were within a 50 residue sequence region, on the cytoplasmic surface of the AChR. Three mAbs crossreacted with mammalian muscle AChRs. Two of them specifically recognized the epsilon subunit of AChRs at adult neuromuscular junction. The epsilon-specific mAbs were used, in conjunction with mAbs specific for the alpha and beta subunits and anti-peptide antisera specific for the epsilon, gamma and delta subunits, to identify in Western blots the subunit complement of embryonic and adult bovine muscle AChRs.
Department of Biochemistry, University of Minnesota, St. Paul 55108.
S
S
Nelson
G D
GD
Shelton
S
S
Lei
J M
JM
Lindstrom
B M
BM
Conti-Tronconi
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D002417:Cattle; D003429:Cross Reactions; D000939:Epitopes; D007120:Immunochemistry; D008969:Molecular Sequence Data; D009132:Muscles / Q000378:metabolism*; D010449:Peptide Mapping; D011950:Receptors, Cholinergic / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism; D014018:Tissue Distribution; D014101:Torpedo / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D000939:Epitopes; D011950:Receptors, Cholinergic