Enkephalin hydrolysis by mouse plasma in vitro.
Hydrolysis of [Leu]- and [Met]enkephalin was determined in samples of pooled whole mouse plasma in vitro by using HPLC-ECD to measure accumulation of Tyr-containing metabolites. More Tyr-Gly-Gly accumulated from [Met]enkephalin than from [Leu]enkephalin hydrolysis, and [Met]enkephalin's half-life in mouse plasma was approximately half that of [Leu]enkephalin. Comparisons of metabolite formation in the presence versus the absence of inhibitors with high selectivity for various peptidases demonstrated that a bestatin-sensitive aminopeptidase, presumably aminopeptidase M, as well as enkephalinase and angiotensin converting enzyme, participate in the hydrolysis of enkephalin in mouse plasma.
Department of Pharmacology, Nihon University School of Medicine, Tokyo, Japan.
S
S
Shibanoki
S B
SB
Weinberger
G
G
Schulteis
K
K
Ishikawa
J L
JL
Martinez
D000595:Amino Acid Sequence; D000626:Aminopeptidases / Q000097:blood; D000818:Animals; D018826:CD13 Antigens; D002851:Chromatography, High Pressure Liquid; D004563:Electrochemistry; D010450:Endopeptidases / Q000097:blood; D004743:Enkephalin, Leucine / Q000097:blood*; D004744:Enkephalin, Methionine / Q000097:blood*; D006868:Hydrolysis; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D015260:Neprilysin / Q000097:blood; D007703:Peptidyl-Dipeptidase A / Q000097:blood; D010949:Plasma / Q000201:enzymology; D013997:Time Factors
D004744:Enkephalin, Methionine; D004743:Enkephalin, Leucine; D010450:Endopeptidases; D000626:Aminopeptidases; D018826:CD13 Antigens; C020071:dipeptidyl carboxypeptidase; D007703:Peptidyl-Dipeptidase A; D015260:Neprilysin