Antitumor activity of some polysaccharides isolated from a Chinese mushroom, "huangmo", the fruiting body of Hohenbuehelia serotina.
Polysaccharides were extracted from a Chinese mushroom, "Huangmo" with hot water (FI), 1% ammonium oxalate solution (FII), and 5% sodium hydroxide (FIII) and 20 more polysaccharides were isolated by ethanol precipitation, ion-exchange chromatography, gel filtration, and affinity chromatography. Of the six polysaccharides obtained, FI0, FA-1, FA-2, FII-2, FIII-1-b, and FIII-2-b were selected on the basis of the results of the antitumor activity test (the Sarcoma 180/mice, i.p. method). Six fractions which showed relatively high activities were examined: FI0, a mixture of galactose-containing heteroglycan and beta-D-glucan; FA-1 and -2, beta-D-glucan with galactose-containing hetero-glycan and protein; FII-2, beta-D-glucan; and FIII-1-b and FIII-2-b, (1----6)-beta-D-glucosyl branched (1----3)-beta-D-glucan.
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Faculty of Basic Medical Science, Norman Bethune University of Medical Sciences, Changchun, Jilin, China.
Mizuno
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Ito
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D000596:Amino Acids / Q000032:analysis; D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D001487:Basidiomycota / Q000737:chemistry*; D005591:Chemical Fractionation; D002686:Chitin / Q000032:analysis; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D008970:Molecular Weight; D010070:Oxalates; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D012513:Sarcoma, Experimental / Q000188:drug therapy; D012972:Sodium Hydroxide; D012995:Solubility
D000596:Amino Acids; D000970:Antineoplastic Agents; D004365:Drugs, Chinese Herbal; D010070:Oxalates; D011134:Polysaccharides; D002686:Chitin; D012972:Sodium Hydroxide