Benarthin: a new inhibitor of pyroglutamyl peptidase. I. Taxonomy, fermentation, isolation and biological activities.
We found benarthin, a new inhibitor of pyroglutamyl peptidase, in the fermentation broth of Streptomyces xanthophaeus MJ244-SF1. It was purified by column chromatography and centrifugal partition chromatography (CPC) and then was isolated as a colorless powder. The binding of benarthin was competitive with substrate and its inhibition constant (Ki) was 1.2 x 10(-6) M.
Aoyagi
T
T
Institute of Microbial Chemistry, Tokyo, Japan.
Hatsu
M
M
Kojima
F
F
Hayashi
C
C
Hamada
M
M
Takeuchi
T
T
D002965:Classification; D004151:Dipeptides / Q000302:isolation & purification* / Q000494:pharmacology; D005285:Fermentation; D011751:Pyroglutamyl-Peptidase I / Q000037:antagonists & inhibitors; D013302:Streptomyces / Q000145:classification*
D004151:Dipeptides; C076470:benarthin; D011751:Pyroglutamyl-Peptidase I