Modifying the mammalian genome by gene targeting.
Over the past year, gene targeting in mammalian cells has become a facile technology. By using a variety of selection and screening protocols, it has become possible to direct modifications at the nucleotide level to specific genes, to target marker genes so that they become expressed under the control of endogenous promoters and to delete large regions of the genome.
Bradley
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Institute for Molecular Genetics, Baylor College of Medicine, Houston, Texas 77030.
D000818:Animals; D004274:DNA, Recombinant; D005822:Genetic Vectors; D016678:Genome; D006801:Humans
D004274:DNA, Recombinant