Hypothalamic corticotropin-releasing hormone and opioid peptide neurons: functional changes after adrenalectomy and/or castration.
The influences of short- and long-term castration and adrenalectomy (or both) upon corticotropin-releasing hormone (CRH) mRNA levels, CRH peptide levels, and endogenous opioid peptide (EOP) content in the hypothalamus, and basal and CRH-stimulated EOP release in vitro, were examined. Gonadal and adrenal steroids regulated the function of these hypothalamic peptidergic systems in terms of peptide synthesis, storage pools, and secretion. The steroids were also found to alter the sensitivity of EOPergic neurons to CRH. In some cases, evidence was obtained for an interaction between gonadal and adrenal steroids in determining neuronal function (seen as additive or counteractive effects). A finding of major importance was that the response of these peptidergic systems was markedly influenced by the duration of steroid deprivation, the results of chronic treatment often being opposite to those of acute treatment. Lastly, inspection of the data on peptide synthesis, storage and release, revealed that there was no simple relationship between these three parameters even within a single type of peptidergic neuron.
Institute of Pharmacology, Toxicology and Pharmacy, Universität München, F.R.G.
O F
OF
Almeida
A H
AH
Hassan
M S
MS
Harbuz
E A
EA
Linton
S L
SL
Lightman
D000315:Adrenalectomy; D000704:Analysis of Variance; D000818:Animals; D003345:Corticosterone / Q000097:blood; D003346:Corticotropin-Releasing Hormone / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D004399:Dynorphins / Q000378:metabolism; D004723:Endorphins / Q000378:metabolism*; D004744:Enkephalin, Methionine / Q000378:metabolism; D007031:Hypothalamus / Q000187:drug effects / Q000502:physiology*; D008297:Male; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D015345:Oligonucleotide Probes; D009919:Orchiectomy; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D012016:Reference Values; D013462:Sulfur Radioisotopes; D001615:beta-Endorphin / Q000378:metabolism
D004723:Endorphins; D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D013462:Sulfur Radioisotopes; D004744:Enkephalin, Methionine; D001615:beta-Endorphin; D004399:Dynorphins; D003346:Corticotropin-Releasing Hormone; D003345:Corticosterone