Improvement of glidobactin A production by Polyangium brachysporum.
Glidobactins A, B, and C are lipopeptide antitumor antibiotics produced by the gliding bacterium Polyangium brachysporum sp. nov. No. K481-B101. The production of glidobactin A was examined in shake flasks and laboratory fermentors. Medium screening and optimization led to approximately five fold increases in glidobactin A titers in shake flasks and a ten fold increase in titers in 40-l batch fermentations. Utilization of a stepped glucose feeding protocol resulted in glidobactin A titers of 1860 micrograms/ml after 144 h of fermentation.
Industrial Division, Bristol-Myers Squibb Co., Syracuse, NY 13221.
J A
JA
Titus
C A
CA
Roundy
D000903:Antibiotics, Antineoplastic / Q000096:biosynthesis*; D050260:Carbohydrate Metabolism; D003470:Culture Media; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008239:Lysine / Q000031:analogs & derivatives / Q000096:biosynthesis; D009231:Myxococcales / Q000254:growth & development / Q000378:metabolism*; D010456:Peptides, Cyclic
D000903:Antibiotics, Antineoplastic; D003470:Culture Media; D010456:Peptides, Cyclic; C057878:glidobactin A; D005947:Glucose; D008239:Lysine