Fat-body-specific expression of the Drosophila Lsp-2 gene.
The larval serum protein-2 gene (Lsp-2) of Drosophila melanogaster is expressed at a very high level in the fat body of third-instar larvae. Here we report that Lsp-2 transcription in adult flies produces a unique mRNA localized in the adult adipose tissue of the head in both sexes. To identify regulatory regions of this Drosophila gene, Lsp-2 5'-flanking DNA sequences were fused to the E. coli beta-galactosidase gene (lacZ). Transient expression of the hybrid gene in third-instar larvae indicates that 230 bp just upstream from the 'TATA box' of the Lsp-2 gene are sufficient for larval fat body-specific expression.
Department of Biochemistry and Molecular Biology, University of Arkansas for Medical Sciences, Little Rock 72205.
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Benes
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Spivey
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Miles
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Neal
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Edmondson
D000818:Animals; D015152:Blotting, Northern; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000235:genetics*; D005216:Fat Body / Q000378:metabolism*; D005260:Female; D007301:Insect Hormones / Q000096:biosynthesis / Q000235:genetics*; D007814:Larva; D008297:Male; D012313:RNA / Q000378:metabolism; D012045:Regulatory Sequences, Nucleic Acid; D015183:Restriction Mapping; D014158:Transcription, Genetic; D014170:Transformation, Genetic
D029721:Drosophila Proteins; D007301:Insect Hormones; C034648:larval serum protein, Drosophila; D012313:RNA