Partial amino acid sequence of a somatostatin receptor isolated from GH4C1 pituitary cells.
A somatostatin receptor isolated from GH4C1 rat pituitary tumor-derived cells was cleaved with cyanogen bromide or cyanogen bromide+trypsin to obtain sequenceable fragments. Five unique amino acid sequences ranging from 6 to 27 amino acid residues were obtained. The sequence was identical to sequence recently reported for one of two somatostatin receptors cloned from human pancreas [Yamada et al., (1992) Proc. Natl. Acad. Sci. U.S.A. 89, 251-255] except for a single valine to isoleucine substitution. This is the first report of amino acid sequence from a purified somatostatin receptor.
Hulmes
J D
JD
American Cyanamid Company: Agricultural Research Division, Princeton, NJ 08540.
Corbett
M
M
Zysk
J R
JR
Böhlen
P
P
Eppler
C M
CM
D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D003488:Cyanogen Bromide; D006801:Humans; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000302:isolation & purification; D010911:Pituitary Neoplasms; D051381:Rats; D017981:Receptors, Neurotransmitter / Q000235:genetics / Q000302:isolation & purification*; D017481:Receptors, Somatostatin; D012689:Sequence Homology, Nucleic Acid; D013004:Somatostatin / Q000378:metabolism; D014357:Trypsin
D010446:Peptide Fragments; D017981:Receptors, Neurotransmitter; D017481:Receptors, Somatostatin; D013004:Somatostatin; D014357:Trypsin; D003488:Cyanogen Bromide