Nuclear incorporation of [adenine 14C]NAD is altered by compounds which affect poly(ADP-ribose) formation.
The use of a DNA alkylating agent, which induces poly(ADP-ribose) formation, has been employed to study the incorporation of [adenine 14C]NAD into pea root meristem nuclei, which is a prerequisite for poly(ADP-ribose) synthesis. The incorporation of [adenine 14C]NAD is significantly reduced when the poly(ADP-ribose)polymerase inhibitors, 7-methylxanthine and 3-methoxybenzamide are present and this incorporation is augmented when the DNA alkylating agent methyl methanesulfonate is added. Such information supports the hypothesis that poly(ADP-ribose) may be involved in the cell cycle regulation of pea root meristem nuclei.
Tramontano
W A
WA
Laboratory of Plant Morphogenesis, Manhattan College, Bronx, NY 10471.
Phillips
D A
DA
Carman
C A
CA
Massaro
A M
AM
D000225:Adenine / Q000378:metabolism; D000470:Alkaloids / Q000494:pharmacology; D001549:Benzamides / Q000494:pharmacology*; D002250:Carbon Radioisotopes; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism*; D004249:DNA Damage; D007887:Fabaceae / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D008741:Methyl Methanesulfonate / Q000494:pharmacology; D009243:NAD / Q000378:metabolism*; D009702:Nucleoside Diphosphate Sugars / Q000096:biosynthesis*; D010946:Plants, Medicinal; D011064:Poly Adenosine Diphosphate Ribose / Q000096:biosynthesis*; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; D014970:Xanthines / Q000494:pharmacology*
D000470:Alkaloids; D001549:Benzamides; D002250:Carbon Radioisotopes; D009702:Nucleoside Diphosphate Sugars; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; D014970:Xanthines; D009243:NAD; D011064:Poly Adenosine Diphosphate Ribose; C009560:trigonelline; D008741:Methyl Methanesulfonate; C064273:7-methylxanthine; D000225:Adenine; C025159:3-methoxybenzamide