[An enzyme study of purine nucleotide biosynthesis in the plerocercoids of cestodes in the fam. Ligulidae].
By means of spectrophotometric method there was determined the activity of three enzymes of biosynthesis of purine nucleotides: amino imidazole ribonucleotide-carboxylase (AIR-carboxylase, EC 4.1.1.21), an enzyme of biosynthesis of purine nucleotides de novo in plerocercoids of Schistocephalus pungitii and Digramma interrupta; inosine monophosphate-dehydrogenase (IMPh-dehydrogenase, EC 1.2.1.14), an enzyme of salvage path, and adenylosuccinate lyase (EC 4.3.2.2), an enzyme taking part both in biosynthesis de novo and salvage in plerocercoids of Schistocephalus pungitii. The activity of AIR-carboxylase was not determined. Specific activities of adenylosuccinate lyase and IMPh-dehydrogenase amount to (1.3 +/- 0.3) x 10(-3) and (1.2 +/- 0.4) x 10(-3) mumole/min.mg protein, respectively. The activity of the three enzymes was determined in the liver of ten-spined stickleback, a host of S. pungitii plerocercoids. The question of metabolic dependence of Ligulidae plerocercoids on hosts to provide for purine bases is discussed.
Dolgikh
V V
VV
Dolgikh
E A
EA
Domkin
V D
VD
D000264:Adenylosuccinate Lyase / Q000032:analysis / Q000378:metabolism; D000818:Animals; D002262:Carboxy-Lyases / Q000032:analysis / Q000378:metabolism; D002589:Cestoda / Q000737:chemistry / Q000201:enzymology*; D002590:Cestode Infections / Q000201:enzymology / Q000469:parasitology / Q000662:veterinary; D005393:Fish Diseases / Q000201:enzymology / Q000469:parasitology; D005399:Fishes; D007168:IMP Dehydrogenase / Q000032:analysis / Q000378:metabolism; D007814:Larva / Q000737:chemistry / Q000201:enzymology; D008099:Liver / Q000737:chemistry / Q000201:enzymology; D011685:Purine Nucleotides / Q000096:biosynthesis*; D013056:Spectrophotometry, Ultraviolet
D011685:Purine Nucleotides; D007168:IMP Dehydrogenase; D002262:Carboxy-Lyases; C022378:phosphoribosylaminoimidazole carboxylase; D000264:Adenylosuccinate Lyase
Izuchenie fermentov biosinteza purinovykh nukleotidov u plevrotserkoidov tsestod sem. Ligulidae.