Endotoxin-induced uveitis in mice. 1. Induction of uveitis and role of T lymphocytes.
Endotoxin-induced uveitis (EIU) with a high frequency of posterior iris synechiae was induced by the systemic injection of 200 micrograms of endotoxin into C3H/HeN mice, an endotoxin-responsive strain. The cell number and the protein concentration within the aqueous humor began to increase 6 hours after the injection, achieving a peak at 24 hours, and decreased gradually thereafter. Inflammatory cells were observed in the anterior chamber, the vitreous body and near the iris-ciliary body histologically. Most of the inflammatory cells were polymorphonuclear cells. On the other hand, C3H/HeJ mice, an endotoxin-unresponsive strain, showed no increase in either cell number or protein concentration in the aqueous humor after endotoxin administration. Pretreatment of C3H/HeN mice with anti-Thy-1.2 antibody significantly decreased both the cell number and the protein concentration in the aqueous humor and the incidence of the posterior synechiae, as compared with the control group. Anti-CD4 antibody also significantly reduced the severity of EIU, while anti-CD8 antibody had no influence on the disease. Anti-IFN-gamma antibody increased the cell number in the aqueous humor. These observations indicate that T lymphocytes, especially CD4+ T lymphocytes, have an extremely important role in the development of EIU in mice.
Department of Ophthalmology, Tokushima University School of Medicine, Japan.
M
M
Kogiso
Y
Y
Tanouchi
Y
Y
Mimura
H
H
Nagasawa
K
K
Himeno
D000818:Animals; D000867:Anterior Chamber / Q000276:immunology / Q000473:pathology; D001427:Bacterial Toxins; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D004195:Disease Models, Animal; D004731:Endotoxins; D004768:Enterotoxins; D005260:Female; D007371:Interferon-gamma / Q000276:immunology; D007518:Isoantibodies / Q000276:immunology; D007958:Leukocyte Count; D051379:Mice; D008809:Mice, Inbred C3H; D009504:Neutrophils / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology; D014605:Uveitis / Q000276:immunology*; D014822:Vitreous Body / Q000276:immunology / Q000473:pathology
D001427:Bacterial Toxins; D004731:Endotoxins; D004768:Enterotoxins; D007518:Isoantibodies; C035678:anti-Thy antibody; C027952:salmonella toxin; D007371:Interferon-gamma