Infectious pancreatic necrosis virus internalization and endocytic organelles in CHSE-214 cells.
The early events that take place during the internalization of infectious pancreatic necrosis virus (IPNV) into Chinook salmon embryo cells (CHSE-214) were analyzed ultrastructurally. Endocytic tracers were employed in order to characterize the organization of endocytic organelles in CHSE-214 cells, as well its relation to the IPNV penetration. Results demonstrate that IPNV appear internalized within vesicular compartments which are located peripherally in CHSE-214 cells. Despite the high rate of infectious multiplicity few virus particles were detected inside the cells. Endocytic tracer labelling of tubulovesicular elements and endosomes of host cells showed a well developed endocytic apparatus. Results suggest that endocytosis may be involved during the initiating events in the productive IPNV infection.
Departamento Biología, Facultad de Medicina, Universidad de Valparaiso, Chile.
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E
Couve
J
J
Kiss
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J
Kuznar
D000818:Animals; D002460:Cell Line; D004705:Endocytosis; D011992:Endosomes / Q000382:microbiology; D005293:Ferritins / Q000378:metabolism; D005393:Fish Diseases / Q000382:microbiology*; D010182:Pancreatic Diseases / Q000662:veterinary*; D012087:Reoviridae / Q000472:pathogenicity*; D012088:Reoviridae Infections / Q000662:veterinary*; D012474:Salmon
D005293:Ferritins