A model for the prediction of partition coefficients in aqueous two-phase systems.
A mathematical model has been developed to predict partition coefficients for aqueous two-phase systems. The model is based on a previously-developed equation for partitioning which arises from an osmotic pressure viral expansion. The model suggests that the properties of importance are the concentration difference of one of the phase-forming components, such as a polymer, and the hydrophobicity of the solute relative to the hydrophobic-hydrophilic difference between the two phases. Several two-phase systems have been studied, with a particular emphasis on the poly(ethylene glycol)/magnesium sulfate system. Numerous solutes, including peptides, were used in this system and their partition coefficients show good agreement with the model.
Eiteman
M A
MA
Center for Bioprocess and Product Development, Department of Chemical Engineering, University of Virginia, Charlottesville 22903-2442.
Gainer
J L
JL
D008433:Mathematics; D008962:Models, Theoretical; D010455:Peptides / Q000302:isolation & purification; D011092:Polyethylene Glycols; D012995:Solubility
D010455:Peptides; D011092:Polyethylene Glycols