Selective flocculation of nucleic acids, lipids, and colloidal particles from a yeast cell homogenate by polyethyleneimine, and its scale-up.
Nucleic acids, lipid, and colloidal particulate material can be selectively flocculated from a yeast cell homogenate by the cationic polymer polyethyleneimine (PEI). Flocculation can occur from a crude homogenate, a homogenate clarified centrifugally, or by the prior use of sodium tetraborate (borax). Flocculation from a homogenate previously clarified by the use of borax is best suited for large-scale operation. The supernatant obtained following centrifugation is effectively free of nucleic acid, lipid, and particulate material with essentially 100% soluble enzyme recovery. Enzyme specific activity increases by approximately 45% compared to a zero PEI control.
Department of Chemical and Biochemical Engineering, University College London, UK.
P
P
Milburn
J
J
Bonnerjea
M
M
Hoare
P
P
Dunnill
D002850:Chromatography, Gel; D003102:Colloids; D004271:DNA, Fungal / Q000302:isolation & purification*; D005427:Flocculation; D005656:Fungal Proteins / Q000302:isolation & purification*; D008055:Lipids / Q000302:isolation & purification*; D011094:Polyethyleneimine; D012331:RNA, Fungal / Q000302:isolation & purification*; D012441:Saccharomyces cerevisiae / Q000032:analysis*
D003102:Colloids; D004271:DNA, Fungal; D005656:Fungal Proteins; D008055:Lipids; D012331:RNA, Fungal; D011094:Polyethyleneimine