[Hydrocortisone Acetate Reference Standard (Control 891) of National Institute of Hygienic Sciences].
The raw material of hydrocortisone acetate was examined for preparation of the "Hydrocortisone Acetate Reference Standard". Analytical results were as follows: E1%1cm (242 nm) 403; IR spectrum indicates specific absorption at 3424, 1745, 1721, 1630, and 1374 cm-1; optical rotation +164.4 degrees (in dioxane); melting point 219.5 degrees (decomposition); loss on drying 0.16%; TLC and HPLC analyses indicated one and three impurities, respectively; assay by HPLC was 99.7% against the previous Reference Standard. Based on the results, the present raw material was authorized to be the Reference Standard of the National Institute of Hygienic Sciences.
S
S
Okada
R
R
Hiroshige
M
M
Tanaka
M
M
Murai
T
T
Kimura
D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D006077:Government Agencies; D006854:Hydrocortisone / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000592:standards; D006920:Hygiene; D007564:Japan; D010602:Pharmacopoeias as Topic; D013055:Spectrophotometry, Infrared
C021650:hydrocortisone acetate; D006854:Hydrocortisone