On-line determination of intracellular beta-galactosidase activity in recombinant Escherichia coli using flow injection analysis (FIA).
A flow injection analysis (FIA) system was developed for the determination of cytoplasmic beta-galactosidase activity in recombinant Escherichia coli. The FIA system and its application for on-line monitoring of beta-galactosidase production during cultivation of recombinant E. coli in a 60-l airlift tower loop reactor is described. The results demonstrate that an FIA assay in conjunction with a cell disintegration step can be applied successfully for on-line monitoring of intracellular protein formation.
Institut für Technische Chemie, Universität Hannover, F.R.G.
H A
HA
Kracke-Helm
L
L
Brandes
B
B
Hitzmann
U
U
Rinas
K
K
Schügerl
D003124:Colorimetry; D004926:Escherichia coli / Q000201:enzymology*; D011995:Recombination, Genetic; D001616:beta-Galactosidase / Q000032:analysis*
D001616:beta-Galactosidase