Effect of endotoxin on serum granulocyte and granulocyte-macrophage colony-stimulating factor levels in dogs.
The biologic effects of endotoxin are attributed to the release of several cytokines, including interleukin-1, interleukin-6, tumor necrosis factor, and the colony-stimulating factors. To investigate the mechanism of endotoxin-induced neutrophilia in dogs, several cell lines known to proliferate selectively in response to recombinant human colony-stimulating factors were examined to determine their responses to recombinant canine granulocyte colony-stimulating factor (rcG-CSF) or recombinant canine granulocyte-macrophage colony-stimulating factor (rcGM-CSF). The murine cell line NFS-60 was found to respond well to rcG-CSF and the human cell line TALL-101 to rcGM-CSF, and these responses were neutralized by antibodies to these recombinant proteins. These bioassays were then used to determine G-CSF and GM-CSF levels in dogs after intravenous endotoxin administration. G-CSF levels increased by 2 h, peaked at 4 h, and had not returned to normal by 24 h after endotoxin. In contrast, GM-CSF was not detectible before or after endotoxin administration.
Department of Medicine, University of Washington, Seattle 98195.
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D000818:Animals; D001427:Bacterial Toxins / Q000494:pharmacology*; D001681:Biological Assay; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D004285:Dogs; D004306:Dose-Response Relationship, Immunologic; D004731:Endotoxins / Q000494:pharmacology*; D004768:Enterotoxins / Q000494:pharmacology*; D016179:Granulocyte Colony-Stimulating Factor / Q000097:blood* / Q000494:pharmacology; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000097:blood* / Q000494:pharmacology; D006801:Humans; D007938:Leukemia; D007951:Leukemia, Myeloid; D009504:Neutrophils / Q000276:immunology*; D011994:Recombinant Proteins / Q000494:pharmacology; D012475:Salmonella; D014407:Tumor Cells, Cultured
D001427:Bacterial Toxins; D004731:Endotoxins; D004768:Enterotoxins; D011994:Recombinant Proteins; C027952:salmonella toxin; D016179:Granulocyte Colony-Stimulating Factor; D016178:Granulocyte-Macrophage Colony-Stimulating Factor