Alpha-D-galactosylation of surface fucoglycoconjugate(s) upon stimulation/activation of murine peritoneal macrophages.
Murine resident macrophages express, on their surface, carbohydrate epitopes which undergo changes during their stimulation/activation as monitored by binding of 125I labelled Evonymus europaea and Griffonia simplicifolia I-B4 lectins. Treatment of the stimulated macrophages with coffee bean alpha-galactosidase abolished binding of the GS I-B4 isolectin and changed the binding pattern of the Evonymus lectin. The affinity (Ka) of Evonymus lectin for alpha-galactosidase-treated macrophages decreased approximately 23-fold, from 1.25 x 10(8) M-1 to 5.5 x 10(6) M-1. Subsequent digestion of alpha-galactosidase-treated macrophages with alpha-L-fucosidase from Trichomonas foetus, further reduced binding of Evonymus lectin. Resident macrophages showed the same pattern of Evonymus lectin binding, with the same affinity, as alpha-galactosidase-treated, stimulated macrophages. These results, together with a consideration of the carbohydrate binding specificity of the Evonymus lectin which, in the absence of alpha-D-galactosyl groups, requires alpha-L-fucosyl groups for binding, indicate the presence, on resident macrophages, of glycoconjugates with terminal alpha-L-fucosyl residues. It is also concluded that during macrophage stimulation/activation alpha-D-galactosyl residues are added to this glycoconjugate and that they form part of the receptor for Evonymus lectin. The same glycoconjugate(s) is/are also expressed on the activated macrophage IC-21 cell line which exhibits the same characteristics as that of stimulated peritoneal macrophages, i.e., it contains alpha-D-galactosyl end groups and is resistant to the action of trypsin. Both lectins were also specifically bound to Corynaebacterium parvum activated macrophages.
Department of Biological Chemistry, University of Michigan, Ann Arbor 48109.
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Petryniak
D000818:Animals; D001665:Binding Sites; D002240:Carbohydrate Sequence; D000939:Epitopes / Q000378:metabolism; D005260:Female; D006001:Glycoconjugates / Q000378:metabolism*; D007457:Iodine Radioisotopes; D037102:Lectins; D008262:Macrophage Activation; D017737:Macrophages, Peritoneal / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D011425:Propionibacterium acnes / Q000276:immunology; D014312:Trisaccharides / Q000378:metabolism; D000519:alpha-Galactosidase / Q000378:metabolism*; D005644:alpha-L-Fucosidase / Q000378:metabolism
D000939:Epitopes; D006001:Glycoconjugates; D007457:Iodine Radioisotopes; D037102:Lectins; D014312:Trisaccharides; C076569:alpha-galactosyl epitope; D000519:alpha-Galactosidase; D005644:alpha-L-Fucosidase