[Vibrio cholerae toxin B subunit gene expressed in a Salmonella vaccine strain].
This paper reports that the V. cholerae toxin B subunit (ctx B) gene was inserted into pYA 248 plasmid with the aspartate beta-semialdehyde dehydrogenase (asd)gene and the recombinant plasmid was transformed into S. typhimurium deleting asd gene. Results showed that ctx B gene was highly expressed and secreted into midium. This strain was able to colonize in the intestinal epithelium. Oral immunity and general immunity could produce antibodies at high level and enhance cellular immune responses. The animals orally inoculated with S. typhimurium x 4072 (pYA-ctx B) vaccine had remarkable protection against virulent V. cholear 569B strain and S. typhimurium strain. Use of such system provides useful method for oral vaccine.
Second Military Medical University, Shanghai.
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Tian
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Lu
D000818:Animals; D001223:Aspartate-Semialdehyde Dehydrogenase / Q000235:genetics; D002772:Cholera Toxin / Q000235:genetics*; D022121:Cholera Vaccines / Q000235:genetics; D003001:Cloning, Molecular; D005260:Female; D015870:Gene Expression; D005798:Genes, Bacterial; D008297:Male; D051379:Mice; D010957:Plasmids; D011817:Rabbits; D012486:Salmonella typhimurium / Q000235:genetics*; D014614:Vaccines, Synthetic / Q000235:genetics
D022121:Cholera Vaccines; D014614:Vaccines, Synthetic; D002772:Cholera Toxin; D001223:Aspartate-Semialdehyde Dehydrogenase