DNA polymorphism of alkaline phosphatase isozyme genes: linkage disequilibria between placental and germ-cell alkaline phosphatase alleles.
The use of human placental alkaline phosphatase (PLAP) cDNA as a probe allows the detection and identification of restriction DNA fragments derived from three homologous genes, i.e., intestinal alkaline phosphatase (AP), germ-cell AP (GCAP), and PLAP. In previous RFLP studies we have reported linkage disequilibria between an RsaI and two PstI (a and b) polymorphic restriction sites and electrophoretic types of PLAP. In this report we present evidence that, in spite of the strong correlation with PLAP types, PstI(b) is an RFLP of GCAP. The data indicate close linkage between the PLAP and GCAP loci.
Department of Medical Genetics, University of Umeå, Sweden.
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G
Beckman
L
L
Beckman
C
C
Sikström
J L
JL
Millán
D000469:Alkaline Phosphatase / Q000235:genetics*; D000483:Alleles; D015139:Blotting, Southern; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D005260:Female; D005854:Germ Cells / Q000201:enzymology*; D006801:Humans; D007527:Isoenzymes / Q000235:genetics; D015810:Linkage Disequilibrium; D010920:Placenta / Q000201:enzymology*; D012150:Polymorphism, Restriction Fragment Length; D011247:Pregnancy
D007527:Isoenzymes; C024388:CTGCAG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific; D000469:Alkaline Phosphatase