High level expression of human tissue-type plasminogen activator gene in mouse C127 cell.
We have expressed human tissue plasminogen activator (t-PA) gene at high levels in a mouse cell line. The t-PA cDNA with deletion of the long 3' untranslated region was inserted into a bovine papilloma virus (BPV) derived vector under the control of a mouse metallothionein promoter. The mouse metallothionein (mMT) gene also provided signals for splicing and polyadenylation. Mouse C127 cells transfected with this construct secreted t-PA at high levels into the cell culture medium. When an SV40 polyadenylation signal was inserted between the t-PA cDNA and the mMT splicing signals, the expression level increased by several fold. The expression levels did not increase further upon either introduction of Rous sarcoma virus LTR into the plasmid or mutation of the translation initiation context sequence to conform with the consensus one. Most of the plasmid appears to be integrated into the host chromosome. Cells producing high levels of t-PA tend to detach from the dish in a few days after passage. When grown on porous microcarriers, however, such cells can be maintained in culture for months and t-PA can be harvested continuously.
Samanta
H K
HK
Eugene Tech International, Inc., Allendale, New Jersey.
Kim
Y J
YJ
D000818:Animals; D002478:Cells, Cultured; D015870:Gene Expression; D006801:Humans; D051379:Mice; D010957:Plasmids; D010959:Tissue Plasminogen Activator / Q000096:biosynthesis / Q000235:genetics*; D014162:Transfection
D010959:Tissue Plasminogen Activator