Expression of protein kinase C in human renal cell carcinoma cells with inherent resistance to doxorubicin.
The expression of protein kinase C (PKC) was analyzed in 18 primary cell cultures of human renal cell carcinomas by means of immunocytochemistry. We found that a high PKC expression significantly correlates with both resistance to doxorubicin and high P-glycoprotein expression. These data support the hypothesis that PKC is involved in inherent drug-resistance by phosphorylation and regulation of P-glycoprotein.
Efferth
T
T
German Cancer Research Center, Heidelberg.
Volm
M
M
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D000911:Antibodies, Monoclonal; D002292:Carcinoma, Renal Cell / Q000201:enzymology* / Q000473:pathology; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D004317:Doxorubicin / Q000494:pharmacology*; D004351:Drug Resistance; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D007150:Immunohistochemistry; D007680:Kidney Neoplasms / Q000201:enzymology* / Q000473:pathology; D008562:Membrane Glycoproteins / Q000032:analysis / Q000378:metabolism*; D011493:Protein Kinase C / Q000032:analysis / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D000911:Antibodies, Monoclonal; D008562:Membrane Glycoproteins; D004317:Doxorubicin; D011493:Protein Kinase C