Cytokine stimulation of multilineage hematopoiesis from immature human cells engrafted in SCID mice.
Severe combined immunodeficient (SCID) mice transplanted with human bone marrow were treated with human mast cell growth factor, a fusion of interleukin-3 and granulocyte-macrophage colony-stimulating factor (PIXY321), or both, starting immediately or 1 month later. Immature human cells repopulated the mouse bone marrow with differentiated human cells of multiple myeloid and lymphoid lineages; inclusion of erythropoietin resulted in human red cells in the peripheral blood. The bone marrow of growth factor-treated mice contained both multipotential and committed myeloid and erythroid progenitors, whereas mice not given growth factors had few human cells and only granulocyte-macrophage progenitors. Thus, this system allows the detection of immature human cells, identification of the growth factors that regulate them, and the establishment of animal models of human hematopoietic diseases.
Department of Genetics, Research Institute, Hospital for Sick Children, Toronto, Ontario, Canada.
T
T
Lapidot
F
F
Pflumio
M
M
Doedens
B
B
Murdoch
D E
DE
Williams
J E
JE
Dick
D000818:Animals; D001854:Bone Marrow Cells; D016026:Bone Marrow Transplantation; D016207:Cytokines / Q000494:pharmacology*; D004921:Erythropoietin / Q000494:pharmacology; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D006410:Hematopoiesis; D016298:Hematopoietic Cell Growth Factors / Q000494:pharmacology; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D007377:Interleukin-3 / Q000494:pharmacology; D051379:Mice; D016513:Mice, SCID; D011993:Recombinant Fusion Proteins / Q000494:pharmacology; D019089:Stem Cell Factor
D016207:Cytokines; D016298:Hematopoietic Cell Growth Factors; D007377:Interleukin-3; D011993:Recombinant Fusion Proteins; D019089:Stem Cell Factor; D004921:Erythropoietin; D016178:Granulocyte-Macrophage Colony-Stimulating Factor