Degradation of isomeric monochlorobenzoates and 2,4-dichlorophenoxyacetic acid by a constructed Pseudomonas sp.
A 4-chlorobenzoate-degrading Pseudomonas sp. US1 was mated with a strain of Escherichia coli JMP 397 (harbouring the plasmid pJP4). An ex-conjugant designated Pseudomonas sp. US1 ex that could utilize all the isomeric monochlorobenzoates and 2,4-dichlorophenoxyacetate was obtained. The ex-conjugant released stoichiometric amounts of chloride when grown on these chloroaromatics as sole sources of carbon and energy.
Sahasrabudhe
A V
AV
Department of Microbiology and Biotechnology Centre, Faculty of Science, M.S. University of Baroda, India.
Modi
V V
VV
D015084:2,4-Dichlorophenoxyacetic Acid / Q000378:metabolism*; D002723:Chlorobenzoates / Q000378:metabolism*; D005818:Genetic Engineering; D006868:Hydrolysis; D010957:Plasmids; D011549:Pseudomonas / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism
D002723:Chlorobenzoates; D015084:2,4-Dichlorophenoxyacetic Acid