Encapsulation and pilus formation of Bacteroides spp. in normal flora abscesses and blood.
The presence of encapsulated and piliated Bacteroides spp. (mostly Bacteroides fragilis and melaninogenicus groups was investigated in isolates from blood, abscesses and normal flora. Of the strains of Bacteroides spp. isolated 45 of 54 (83%) recovered from blood and 31 of 40 (78%) found in abscesses were encapsulated. In contrast, only seven of 71 (10%) similar strains isolated from the faeces or pharynx of healthy persons were encapsulated (P < 0.001). Pili were observed in three of 54 (6%) of strains isolated from blood, 30 of 40 (75%) of those recovered from abscesses (P < 0.001), and 49 of 71 (69%) of those found in normal flora (P < 0.001). The predominance of encapsulated forms in all strains of B. fragilis and B. melaninogenicus in blood as well as in abscesses suggests an increased virulence of these compared with non-encapsulated isolates. In contrast, the presence of pili in Bacteroides spp. recovered mostly from abscesses and normal flora suggests that this structure may play a role in the ability of these organisms to adhere to mucous membranes and may interfere with their ability to spread systemically. These findings illustrate the morphological differences that may be observed in Bacteroides spp. from various anatomical sites.
Brook
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Naval Medical Research Institute, Bethesda, Maryland.
Myhal
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Dorsey
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D000038:Abscess / Q000382:microbiology; D000293:Adolescent; D000328:Adult; D000368:Aged; D016470:Bacteremia / Q000382:microbiology; D016667:Bacterial Capsules / Q000302:isolation & purification*; D001439:Bacteroides / Q000302:isolation & purification / Q000502:physiology*; D001442:Bacteroides Infections / Q000382:microbiology*; D002648:Child; D005260:Female; D010861:Fimbriae, Bacterial / Q000502:physiology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D011446:Prospective Studies; D013045:Species Specificity