Production of recombinant Von Willebrand factor by CHO cells cultured in macroporous microcarriers.
Recombinant Chinese hamster ovary cells producing Von Willebrand factor have been successfully grown in gelatin macroporous microcarriers (Cultispher-G). Serum-free cultures were maintained in 1, 4, and 10 liter fermentors for more than two months. Comparative studies with Cytodex-3 microcarriers have been performed in 1 liter fermentors. The lower specific Von Willebrand factor productivity of CHO cells cultivated on Cultispher-G were offset by higher cell densities (10(7) - 2 x 10(7) cells/ml). Volumetric Von Willebrand factor productivity was influenced by oxygen concentration, and remained stable during scale-up from 1 to 10 liter fermentors.
Centre National de Transfusion Sanguine, Les Ulis, France.
G
G
Mignot
T
T
Faure
V
V
Ganne
B
B
Arbeille
A
A
Pavirani
J L
JL
Romet-Lemonne
D000818:Animals; D001709:Biotechnology / Q000295:instrumentation / Q000379:methods; D002460:Cell Line; D003911:Dextrans; D005780:Gelatin; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D010539:Permeability; D011994:Recombinant Proteins / Q000096:biosynthesis; D014841:von Willebrand Factor / Q000096:biosynthesis*
D003911:Dextrans; D011994:Recombinant Proteins; D014841:von Willebrand Factor; C052839:Cytodex; D005780:Gelatin