An intergenic G-rich region in Leishmania tarentolae kinetoplast maxicircle DNA is a pan-edited cryptogene encoding ribosomal protein S12.
Six short G-rich intergenic regions in the maxicircle of Leishmania tarentolae are conserved in location and polarity in two other kinetoplastid species. We show here that G-rich region 6 (G6) represents a pan-edited cryptogene which contains at least two domains edited independently in a 3'-to-5' manner connected by short unedited regions. In the completely edited RNA, 117 uridines are added at 49 sites and 32 uridines are deleted at 13 sites, creating a translated 85-amino-acid polypeptide. Similar polypeptides are probably encoded by pan-edited G6 transcripts in two other species. The G6 polypeptide has significant sequence similarity to the family of S12 ribosomal proteins. A minicircle-encoded gRNA overlaps 12 editing sites in G6 mRNA, and chimeric gRNA/mRNA molecules were shown to exist, in agreement with the transesterification model for editing.
Maslov
D A
DA
Department of Biology, University of California, Los Angeles 90024.
Sturm
N R
NR
Niner
B M
BM
Gruszynski
E S
ES
Peris
M
M
Simpson
L
L
D000595:Amino Acid Sequence; D000818:Animals; D001482:Base Composition; D001483:Base Sequence; D015152:Blotting, Northern; D002478:Cells, Cultured; D004270:DNA, Circular / Q000235:genetics*; D018105:DNA, Kinetoplast; D016054:DNA, Protozoan / Q000235:genetics*; D006147:Guanine; D007438:Introns; D007891:Leishmania / Q000235:genetics*; D008969:Molecular Sequence Data; D011061:Poly A; D012313:RNA; D012323:RNA Processing, Post-Transcriptional; D017394:RNA, Guide, Kinetoplastida; D016053:RNA, Protozoan / Q000235:genetics / Q000378:metabolism; D012269:Ribosomal Proteins / Q000235:genetics*; D016415:Sequence Alignment
D004270:DNA, Circular; D018105:DNA, Kinetoplast; D016054:DNA, Protozoan; D016053:RNA, Protozoan; D012269:Ribosomal Proteins; C034212:ribosomal protein S12; D011061:Poly A; D006147:Guanine; D012313:RNA; D017394:RNA, Guide, Kinetoplastida