Detection of human T-cell leukemia/lymphoma virus, type II, in a patient with large granular lymphocyte leukemia.
We studied a patient with large granular lymphocyte (LGL) leukemia for evidence of human T-cell leukemia/lymphoma virus (HTLV) infection. Serum from this patient was positive for HTLV-I/II antibodies by enzyme-linked immunosorbent assay (ELISA) and was confirmed positive in Western blot and radioimmunoprecipitation assays. Results of a synthetic peptide-based ELISA showed that the seropositivity was caused by HTLV-II and not HTLV-I infection. Analyses of enzymatic amplification of DNA from bone marrow sections using the polymerase chain reaction (PCR) were positive for HTLV-II specific gag, pol, env, and pX gene sequences. Cloning and sequencing of amplified products showed that the HTLV-II pol and pX sequences in patient DNA differed from the sequences of 17 other HTLV-II isolates examined in our laboratory. HTLV infection may have a role in some patients in the pathogenesis of LGL leukemia.
Fred Hutchinson Cancer Research Center, Seattle, WA.
T P
TP
Loughran
T
T
Coyle
M P
MP
Sherman
G
G
Starkebaum
G D
GD
Ehrlich
F W
FW
Ruscetti
B J
BJ
Poiesz
D001483:Base Sequence; D004279:DNA, Viral / Q000032:analysis; D015480:Deltaretrovirus Antibodies / Q000032:analysis; D006800:Deltaretrovirus Infections / Q000382:microbiology*; D015368:Human T-lymphotropic virus 1 / Q000235:genetics / Q000276:immunology; D015367:Human T-lymphotropic virus 2 / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans; D007945:Leukemia, Lymphoid / Q000382:microbiology*; D008969:Molecular Sequence Data
D004279:DNA, Viral; D015480:Deltaretrovirus Antibodies