Mini- and micro-satellites in the genome of rodent malaria parasites.
Higher eukaryotes contain within their DNA numerous arrays of repetitive DNA, many of which are known as satellite DNAs and display extensive variability. The presence of these repeats has been demonstrated for various species and they have been used for genetic identification and classification. Here, it is demonstrated that Southern hybridisation of DNA from rodent malaria parasites allows detection of micro- and minisatellite sequences in the genome of Plasmodium species. Closely related lines of malaria parasites exhibit a monomorphic hybridisation pattern, which is in contrast to the allelic variation observed in higher eukaryotes. Among different species, however, restriction-fragment length polymorphism was observed. Pulsed-field gel electrophoretic chromosome separation showed that the probes used in this study [33.15, 33.6, (CAC)n and (GT)n] detect several loci spread over different chromosomes.
Diagnostic Centre SSDZ, Department Molecular Biology, Delft, The Netherlands.
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A
van Belkum
J
J
Ramesar
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G
Trommelen
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AG
Uitterlinden
D000483:Alleles; D000818:Animals; D015139:Blotting, Southern; D002874:Chromosome Mapping; D016172:DNA Fingerprinting; D016054:DNA, Protozoan / Q000145:classification / Q000235:genetics*; D004276:DNA, Satellite / Q000235:genetics*; D016521:Electrophoresis, Gel, Pulsed-Field; D010961:Plasmodium / Q000145:classification / Q000235:genetics*; D010962:Plasmodium berghei / Q000145:classification / Q000235:genetics; D012150:Polymorphism, Restriction Fragment Length; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*; D012377:Rodentia / Q000469:parasitology
D016054:DNA, Protozoan; D004276:DNA, Satellite