Two intragenic polymorphisms of the APC-gene detected by PCR and enzymatic digestion.
The gene causing adenomatous polyposis coli (APC) has recently been cloned. Three intragenic polymorphisms were reported to be detectable by single-strand conformation polymorphism analysis. Here, we describe an assay using polymerase-chain-reaction-based amplification and subsequent enzymatic digestion of genomic sequences to identify polymorphic sites at nucleotide positions 1458 and 5037 of the APC gene.
Kraus
C
C
Institut für Humangenetik der Universität, Erlangen, Federal Republic of Germany.
Ballhausen
W G
WG
D011125:Adenomatous Polyposis Coli / Q000235:genetics*; D001483:Base Sequence; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length