Stimulative effect of non-parenchymal liver cells on ability of tyrosine aminotransferase induction in hepatocytes.
Hepatocytes and non-parenchymal liver cells were isolated from adult rat liver and co-cultured for 48 hours as a monolayer on polystyrene culture dishes. The ability of tyrosine aminotransferase (TAT) induction in hepatocytes was examined in the presence of dexamethasone and dibutyryl cAMP. Non-parenchymal cells greatly enhance the ability of TAT induction of hepatocytes. A soluble factor with molecular weight of more than 10,000 is responsible for this enhancement, because conditioned medium prepared from non-parenchymal cells is also stimulatory. Non-parenchymal cells restored the ability in hepatocytes damaged with the addition of D-galactosamine. Conditioned medium prepared from non-parenchymal cells treated with D-galactosamine had higher activity of enhancement than the medium from normal cells. The soluble factor might be released in response to some signal of injury. Hepatocytes and non-parenchymal cells were immobilized within Ca-alginate, and although immobilized hepatocytes rapidly lost the ability to induce TAT, hepatocytes co-immobilized with non-parenchymal cells maintained the ability during 4 days of culture. These results indicated that non-parenchymal liver cells, as well as hepatocytes, could be used to construct a bioartificial liver support system.
Faculty of Pharmaceutical Sciences, Osaka University, Japan.
K
K
Yagi
N
N
Suenobu
M
M
Serada
K
K
Tsuda
A
A
Kondoh
Y
Y
Miura
D000818:Animals; D002450:Cell Communication; D002478:Cells, Cultured; D017077:Culture Media, Conditioned; D046508:Culture Techniques / Q000379:methods; D004790:Enzyme Induction; D007700:Kinetics; D008099:Liver / Q000166:cytology / Q000201:enzymology / Q000502:physiology*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D014444:Tyrosine Transaminase / Q000096:biosynthesis*
D017077:Culture Media, Conditioned; D014444:Tyrosine Transaminase