Preparation of peptide mixture with high Fischer ratio from protein hydrolysate by adsorption on activated carbon.
A peptide mixture with a high Fischer ratio (a molar ratio of Val + Leu + Ile to Phe + Tyr) was prepared by the adsorptive separation of a casein hydrolysate by activated carbon. The effects of the pH and ethanol content of the hydrolysate on the Fischer ratio and on the yield of the resulting peptide mixture were examined. A peptide mixture with the Fischer ratio of 31.6 was obtained at pH 2.5 without the addition of ethanol. The Fischer ratio was close to the ratio of the infusion solution of free amino acids that is now used for patients with liver diseases.
Department of Food Science & Technology, Faculty of Agriculture, Kyoto University, Japan.
S
S
Adachi
T
T
Yamanaka
S
S
Hayashi
Y
Y
Kimura
R
R
Matsuno
H
H
Yokogoshi
D000327:Adsorption; D000596:Amino Acids / Q000032:analysis; D000597:Amino Acids, Branched-Chain / Q000032:analysis; D002606:Charcoal / Q000737:chemistry*; D006868:Hydrolysis; D010455:Peptides / Q000138:chemical synthesis*; D011506:Proteins / Q000737:chemistry*
D000596:Amino Acids; D000597:Amino Acids, Branched-Chain; D010455:Peptides; D011506:Proteins; D002606:Charcoal