Production of human interleukin-3 using industrial microorganisms.
We expressed a cDNA encoding the multicolony stimulating factor interleukin-3 in a variety of cell types, including bacteria, yeast and mammalian cells. After evaluation of the advantages and disadvantages of each potential system, we designed a production and purification scheme using Bacillus licheniformis. The purification consists of hydrophobic interaction chromatography, two steps of ion exchange chromatography and gel filtration. The purified and formulated product entered clinical trials in November 1989.
van Leen
R W
RW
Royal Gist-brocades NV, Delft, The Netherlands.
Bakhuis
J G
JG
van Beckhoven
R F
RF
Burger
H
H
Dorssers
L C
LC
Hommes
R W
RW
Lemson
P J
PJ
Noordam
B
B
Persoon
N L
NL
Wagemaker
G
G
D000818:Animals; D001407:Bacillus / Q000235:genetics / Q000378:metabolism; D001709:Biotechnology / Q000379:methods; D002478:Cells, Cultured; D004247:DNA / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007377:Interleukin-3 / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification; D008322:Mammals; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification
D007377:Interleukin-3; D011994:Recombinant Proteins; D004247:DNA