Immunohistochemical characterization of endocrine cells in experimental exocrine pancreatic cancer in the Syrian golden hamster.
Fifty exocrine pancreatic adenocarcinomas and 57 benign tumors induced in Syrian hamsters by N-nitrosobis(2-oxopropyl)amine (BOP) were examined for the presence of argyrophil cells antiinsulin, -glucagon, -somatostatin, -pancreatic polypeptide (PP), -gastrin/CCK, -vasoactive intestinal polypeptide (VIP), and - neuron-specific enolase (NSE) reactive cells. Argyrophil - and antihormone-reactive cells were found in the normal pancreatic ducts and in the acini, as well as in hyperplastic and atypical ducts/ductules, tubular complexes, benign lesions, and in 80% of ductal adenocarcinomas. Insulin and antiNSE-reactive cells were the most common, followed in decreasing frequency by glucagon, somatostatin, and PP cells. Antigastrin-/CCK-and -VIP-reactive cells were found in two cases. Argyrophil cells were present in about 60% of the tumors with Grimelius staining and in 55% of those with Churukian-Schenk staining. Insulin cells were seen in ductal cancer that had grown into a lymph node and in the lymph node metastases of another cancer. A novel finding was the presence of argyrophil and insulin cells within the lumen of some malignant glandular structures. Coexistence of several peptide cells was found in 52% of the cancers. The presence of argyrophil and hormone-producing cells in induced pancreatic ductal/ductular lesions further strengthens the existence of a close developmental relationship between exocrine and endocrine cells of the pancreas.
Department of Pathology, Lund University, Sweden.
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S
Dawiskiba
P M
PM
Pour
U
U
Stenram
F
F
Sundler
A
A
Andrén-Sandberg
D000230:Adenocarcinoma / Q000737:chemistry / Q000473:pathology*; D000818:Animals; D006224:Cricetinae; D005260:Female; D005755:Gastrins / Q000032:analysis; D005934:Glucagon / Q000032:analysis; D007150:Immunohistochemistry; D007328:Insulin / Q000032:analysis; D007515:Islets of Langerhans / Q000737:chemistry / Q000473:pathology*; D008297:Male; D008647:Mesocricetus; D010183:Pancreatic Ducts / Q000737:chemistry / Q000473:pathology; D010190:Pancreatic Neoplasms / Q000737:chemistry / Q000473:pathology*; D010191:Pancreatic Polypeptide / Q000032:analysis; D010751:Phosphopyruvate Hydratase / Q000032:analysis; D016622:Silver Staining; D013004:Somatostatin / Q000032:analysis; D014660:Vasoactive Intestinal Peptide / Q000032:analysis
D005755:Gastrins; D007328:Insulin; D014660:Vasoactive Intestinal Peptide; D013004:Somatostatin; D010191:Pancreatic Polypeptide; D005934:Glucagon; D010751:Phosphopyruvate Hydratase