Detection by fluorescence analysis of DNA unwinding and unscheduled DNA synthesis, of DNA damage and repair induced in vitro by direct-acting mutagens on human lymphocytes.
The sensitivity and reliability of UDS and FADU in detecting mutagenic effects were compared by measuring DNA damage and repair in PBL treated in vitro with UV light, MMS and BPDE. The results indicate that FADU is more sensitive than UDS, as it is able to detect DNA damage at doses 3-4-fold lower. We also determined the DNA damage and repair induced by the above agents on lymphocyte samples from different donors by FADU and UDS, confirming that the DNA repair process in humans is characterized by interindividually variable efficiency.
Department of Biology, University of Padua, Italy.
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Celotti
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Ferraro
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Biasin
D001580:Benzopyrenes / Q000633:toxicity; D004247:DNA / Q000187:drug effects* / Q000528:radiation effects*; D004249:DNA Damage; D004260:DNA Repair / Q000187:drug effects* / Q000528:radiation effects*; D004261:DNA Replication / Q000187:drug effects* / Q000528:radiation effects*; D004305:Dose-Response Relationship, Drug; D004307:Dose-Response Relationship, Radiation; D005455:Fluorescent Antibody Technique; D006801:Humans; D066298:In Vitro Techniques; D008741:Methyl Methanesulfonate / Q000009:adverse effects; D009153:Mutagens / Q000009:adverse effects*; D013997:Time Factors; D014466:Ultraviolet Rays / Q000009:adverse effects
D001580:Benzopyrenes; D009153:Mutagens; D004247:DNA; D008741:Methyl Methanesulfonate