Controlled release of interleukin-2 from biodegradable microspheres.
We have evaluated the use of biodegradable poly(DL-lactide-co-glycolide) microspheres for the controlled release of interleukin-2 (IL-2) and its modified forms: succinyl IL-2 (SIL-2) and polyethylene glycol-modified IL-2 (PEG IL-2). We show that a microsphere formulation can be prepared from PEG IL-2 using HSA as an excipient which, after an initial burst, releases 2-3% PEG IL-2 per day in a bioactive form continuously over a 20- to 30-day period.
Hora
M S
MS
Cetus Corporation, Emeryville, CA 94608.
Rana
R K
RK
Nunberg
J H
JH
Tice
T R
TR
Gilley
R M
RM
Hudson
M E
ME
D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D066298:In Vitro Techniques; D007376:Interleukin-2 / Q000031:analogs & derivatives / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D011092:Polyethylene Glycols; D011098:Polyglactin 910; D011994:Recombinant Proteins / Q000737:chemistry; D012709:Serum Albumin / Q000737:chemistry; D012995:Solubility
D007376:Interleukin-2; D011994:Recombinant Proteins; D012709:Serum Albumin; C067245:interleukin-2, polyethylene glycol-modified; C067246:interleukin-2, succinyl-; D011098:Polyglactin 910; D011092:Polyethylene Glycols