Separation processes in biotechnology. Precipitation.
Precipitation has a clear role in downstream processing for product concentration, and ongoing developments promise a more important role in fractionation. Its advantages include adaptability to continuous processing and larger scales, the wide variety of possible precipitants, and the ability to retain biological activity. The focus here has been on precipitation of proteins, but many of the principles apply to nucleic acid removal and precipitation of lower-molecular-weight biological products, such as pharmaceuticals.
Glatz
C E
CE
Iowa State University, Ames.
D001709:Biotechnology / Q000379:methods; D011232:Chemical Precipitation; D004573:Electrolytes; D007526:Isoelectric Point; D011506:Proteins / Q000302:isolation & purification; D012997:Solvents
D004573:Electrolytes; D011506:Proteins; D012997:Solvents