DnaK-mediated alterations in human growth hormone protein inclusion bodies.
Protein overproduction in microbes frequently results in protein misfolding and aggregation though the molecular basis for this process is unclear. The HSP70 chaperonin, DnaK, was identified as an important factor controlling heterologous protein aggregation in Escherichia coli. Co-overproduction of DnaK significantly reduced human growth hormone (HGH) protein inclusion body formation and the extent of HGH aggregation.
Department of Microbiology and Immunology, Stanford University School of Medicine, California 94305-5402.
P
P
Blum
M
M
Velligan
N
N
Lin
A
A
Matin
D001426:Bacterial Proteins / Q000378:metabolism*; D002457:Cell Extracts / Q000302:isolation & purification; D002458:Cell Fractionation; D018833:Chaperonins; D004926:Escherichia coli / Q000378:metabolism*; D013006:Growth Hormone / Q000737:chemistry*; D046911:Macromolecular Substances; D010957:Plasmids / Q000235:genetics; D011487:Protein Conformation; D011506:Proteins / Q000502:physiology*; D012995:Solubility
D001426:Bacterial Proteins; D002457:Cell Extracts; D046911:Macromolecular Substances; D011506:Proteins; D013006:Growth Hormone; D018833:Chaperonins