Detection and separation of two kinds of acidic arginine amidases from boar sperm using lima bean trypsin inhibitor and aprotinin affinity adsorptions.
Two kinds of acidic arginine amidase activity were found in boar sperm. One enzyme was separated by a treatment consisting of lima bean trypsin inhibitor (LBTI) affinity adsorption and elution. The other enzyme was separated by aprotinin affinity adsorption and elution through the same solutions as those used for first enzyme; the two enzymes provisionally named boar sperm acidic arginine amidases 1 (BSAA-1) and 2 (BSAA-2), respectively. The amidolytic activity of BSAA-1 was increased by high concentrations of calcium chloride, while the activity of BSAA-2 was independent of calcium chloride. Their behavior with LBTI and aprotinin, and profiles of their substrate specificities, were also different. The affinity of LBTI to BSAA-1 was approximately 14 times higher than that to BSAA-2.
Department of Biochemistry, Meiji College of Pharmacy, Tokyo, Japan.
T
T
Kobayashi
I
I
Hara
Y
Y
Matsuda
J Y
JY
Park
S
S
Oshio
S
S
Kaneko
T
T
Umeda
S
S
Akihama
D000327:Adsorption; D000595:Amino Acid Sequence; D000818:Animals; D007611:Aprotinin; D002122:Calcium Chloride / Q000494:pharmacology; D002846:Chromatography, Affinity; D004305:Dose-Response Relationship, Drug; D008297:Male; D008969:Molecular Sequence Data; D012697:Serine Endopeptidases / Q000032:analysis*; D013053:Spectrophotometry; D013094:Spermatozoa / Q000201:enzymology*; D013379:Substrate Specificity; D013552:Swine; D014361:Trypsin Inhibitors
D014361:Trypsin Inhibitors; D007611:Aprotinin; D012697:Serine Endopeptidases; C073652:acidic arginine amidase-1, boar sperm; C073653:acidic arginine amidase-2, boar sperm; D002122:Calcium Chloride