Degradation and dehalogenation of monochlorophenols by the phenol-assimilating yeast Candida maltosa.
The phenol-assimilating yeast Candida maltosa is able to degrade monochlorophenols but cannot grow on these substrates. 3- and 4-chlorophenol were broken down very rapidly by phenol-grown cells under the formation of 4-chlorocatechol, 5-chloropyrogallol and 4-carboxymethylenebut-2-en-4-olide with concomitant release of chloride. 2-Chlorophenol was partially converted into cis,cis-2-chloromuconic acid via 3-chlorocatechol which was also obtained from 3-chlorophenol in low amounts. No further metabolites containing chloride were found. The dehalogenation step in the chlorophenol degradation is the cycloisomerization of the cis,cis-chloromuconic acid to 4-carboxymethylenebut-2-en-4-olide in the ortho fission pathway.
Polnisch
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Institut für Angewandte und Technische Mikrobiologie, Ernst-Moritz-Arndt-Universität Greifswald, Germany.
Kneifel
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Franzke
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Hofmann
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KH
D001673:Biodegradation, Environmental; D001709:Biotechnology; D002175:Candida / Q000254:growth & development / Q000378:metabolism*; D002733:Chlorophenols / Q000737:chemistry / Q000378:metabolism*; D007700:Kinetics; D006899:Mixed Function Oxygenases / Q000378:metabolism
D002733:Chlorophenols; D006899:Mixed Function Oxygenases; C022336:phenol 2-monooxygenase