Real time automated simultaneous double-stranded DNA sequencing using two-color fluorophore labeling.
We determined the base sequences of both strands of duplex DNA simultaneously with an automated DNA sequencer. Primers for each strand were labeled with two different dyes and chain extension reactions for both strands produced four DNA families: "A", "C", "G", and "T". An image splitting-prism and band-pass filters were mounted on a real time DNA sequencer to detect fluorescences from the two different dyes separately. As a result, one thousand bases of a clone could be determined at the same time.
Central Research Laboratory, Hitachi Ltd., Tokyo, Japan.
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Kambara
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Nagai
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Hayasaka
D001331:Automation; D001483:Base Sequence; D003116:Color; D003199:Computer Systems; D004247:DNA / Q000737:chemistry*; D005456:Fluorescent Dyes; D008969:Molecular Sequence Data; D013050:Spectrometry, Fluorescence
D005456:Fluorescent Dyes; D004247:DNA