Effect of 2-deoxy-D-glucose on DNA double strand break repair, cell survival and energy metabolism in euoxic Ehrlich ascites tumour cells.
Effects of 2-deoxy-D-glucose (2-DG) on DNA double strand break (dsb) repair, cell survival and on the energy metabolism were investigated in exponentially growing Ehrlich ascites tumour (EAT) cells. Cells in suspension were exposed to 40 Gy of X-rays and allowed to repair (up to 4 h) with or without 2-DG at 37 degrees C. DNA dsb rejoining was measured by means of clamped homogeneous electric field (CHEF), a pulsed field gel electrophoresis technique. The fraction of activity released (FAR) during electrophoresis (DNA associated 14C-thymidine) was used as a parameter to determine the number of dsb present in the DNA. Biphasic kinetics for dsb repair were observed. The presence of 2-DG significantly inhibited the slow component of dsb repair. The presence of 2-DG also enhanced radiation-induced cell killing. ATP content of cells was measured by a bioluminescence method. ATP content in exponentially growing cells was about 4 pg per cell. The level of ATP was reduced by 50% in presence of 2-DG (C2-DG/CG = 1.0).
Jha
B
B
Botany Department, L. N. Mithila University, Darbhanga, India.
Pohlit
W
W
D000255:Adenosine Triphosphate / Q000032:analysis; D000818:Animals; D002286:Carcinoma, Ehrlich Tumor; D002470:Cell Survival / Q000187:drug effects*; D004247:DNA / Q000528:radiation effects*; D004260:DNA Repair / Q000187:drug effects*; D003847:Deoxyglucose / Q000494:pharmacology*; D016521:Electrophoresis, Gel, Pulsed-Field; D004734:Energy Metabolism / Q000187:drug effects*; D011836:Radiation Tolerance; D013997:Time Factors
D000255:Adenosine Triphosphate; D004247:DNA; D003847:Deoxyglucose