Large-scale purification and preliminary structural studies of MAC 182, a gibberellin-binding monoclonal antibody.
The large-scale purification of the anti-gibberellin monoclonal antibody, MAC 182, is described. N-Terminal amino acid sequences of the heavy and light chains were determined and compared with those of known antibodies. Fab-fragments were prepared and purified to a state suitable for crystallization.
Department of Agricultural Sciences, University of Bristol, AFRC Institute of Arable Crops Research, U.K.
J E
JE
Nester-Hudson
M H
MH
Beale
J
J
MacMillan
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*; D005875:Gibberellins / Q000276:immunology*; D006825:Hybridomas; D007140:Immunoglobulin Fab Fragments / Q000302:isolation & purification; D007143:Immunoglobulin Heavy Chains / Q000737:chemistry; D007147:Immunoglobulin Light Chains / Q000737:chemistry; D008969:Molecular Sequence Data; D051381:Rats
D000911:Antibodies, Monoclonal; D005875:Gibberellins; D007140:Immunoglobulin Fab Fragments; D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains