Surface presentation of protein epitopes using bacteriophage expression systems.
Vast libraries of filamentous phage expression vectors that display foreign (poly)peptides on the virion surface can be screened by affinity-purifying those phage whose displayed foreign peptide binds to an antibody or another binding protein. Present libraries display only short random peptides, but work is presently underway to create libraries displaying antibodies with a great diversity of binding specificities.
Smith
G P
GP
University of Missouri, Columbia.
D000595:Amino Acid Sequence; D000914:Antibodies, Viral; D001435:Bacteriophages / Q000235:genetics*; D002213:Capsid / Q000235:genetics; D000939:Epitopes / Q000235:genetics*; D015723:Gene Library; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D011506:Proteins / Q000235:genetics*
D000914:Antibodies, Viral; D000939:Epitopes; D011506:Proteins