Extracellular DNA level in the blood of irradiated rats.
There is high-molecular DNA in the blood of unirradiated rats which moves as a single fraction during electrophoresis in 0.5% agarose. At short times (2-5 h) after gamma-irradiation at doses from 1 to 100 Gy a low-molecular species of DNA appears (about 180 nucleotide pairs), the amount of which is directly proportional to exposure dose at 5 h after exposure. It has been established by Southern hybridization that the low-molecular DNA has few nucleotide sequences common with those of the high-molecular DNA, but it shows homologously to genomic sequences.
Scientific Research Institute of Military Medicine, USSR Defence Ministry, St Petersburg, Commonwealth of Independent States.
V G
VG
Vladimirov
A S
AS
Belokhvostov
S S
SS
Sherlina
I N
IN
Vasilyeva
A M
AM
Voskresensky
D000818:Animals; D002588:Cesium Radioisotopes; D004247:DNA / Q000097:blood*; D005110:Extracellular Space; D005720:Gamma Rays; D008297:Male; D011831:Radiation Genetics; D011833:Radiation Injuries, Experimental / Q000097:blood* / Q000235:genetics; D051381:Rats; D014916:Whole-Body Irradiation
D002588:Cesium Radioisotopes; D004247:DNA