H-2M3 presents a Listeria monocytogenes peptide to cytotoxic T lymphocytes.
We report evidence that a major histocompatibility complex-encoded nonclassic class I molecule presents a foreign peptide to cytotoxic T lymphocytes (CTL) during an infection. Mice immunized with virulent Listeria monocytogenes generate CD8+ CTL with alpha beta receptors specific for a bacterial peptide presented by a conserved class I molecule encoded in the M region of the major histocompatibility complex. The Listeria peptide is digested by carboxypeptidase Y but resists aminopeptidase M, and only peptides with N-formyl methionine competitively block its presentation to CTL. Transfection with the H-2M3d gene enables a negative (H-2w17) cell line to present the bacterial peptide. One function, therefore, of H-2M3 is to present bacterial peptides to CTL during infection.
Howard Hughes Medical Institute, Department of Immunology, Seattle, Washington.
E G
EG
Pamer
C R
CR
Wang
L
L
Flaherty
K F
KF
Lindahl
M J
MJ
Bevan
D000595:Amino Acid Sequence; D000626:Aminopeptidases / Q000737:chemistry; D000818:Animals; D000942:Antigens, Bacterial / Q000737:chemistry / Q000276:immunology*; D001667:Binding, Competitive; D018826:CD13 Antigens; D016827:CD8 Antigens / Q000276:immunology; D002268:Carboxypeptidases / Q000737:chemistry; D002460:Cell Line; D008089:Listeria monocytogenes / Q000276:immunology*; D008285:Major Histocompatibility Complex / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D009239:N-Formylmethionine / Q000378:metabolism; D010455:Peptides / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000166:cytology / Q000276:immunology*
D000942:Antigens, Bacterial; D016827:CD8 Antigens; D010455:Peptides; D009239:N-Formylmethionine; D002268:Carboxypeptidases; D000626:Aminopeptidases; D018826:CD13 Antigens; C022633:serine carboxypeptidase