Regulation of activin beta A mRNA level by cAMP.
We demonstrated the presence of five species of the activin beta A mRNA in human placenta and one major RNA associated with two minor RNAs of the activin in the fetal membrane. We investigated the effect of 8-bromo-cAMP (8-Br-cAMP) on accumulation of activin beta A subunit mRNA in human fibrosarcoma HT1080 cells. Although low levels of the activin mRNA were detectable in the untreated cells, the one main RNA species was predominantly accumulated by 8-Br-cAMP. We propose that generation of multiple activin mRNAs in the fetal membrane and cAMP-treated HT1080 cells is presumably due to a cell-specific alternative polyadenylation.
Institute of Applied Biochemistry, University of Tsukuba, Ibaraki, Japan.
K
K
Tanimoto
K
K
Tamura
N
N
Ueno
S
S
Usuki
K
K
Murakami
A
A
Fukamizu
D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000494:pharmacology*; D028341:Activins; D015152:Blotting, Northern; D002460:Cell Line; D000242:Cyclic AMP / Q000502:physiology*; D005321:Extraembryonic Membranes / Q000502:physiology; D005260:Female; D005354:Fibrosarcoma; D005786:Gene Expression Regulation / Q000187:drug effects; D006367:HeLa Cells; D006801:Humans; D007265:Inhibins / Q000096:biosynthesis / Q000235:genetics*; D007700:Kinetics; D010920:Placenta / Q000502:physiology*; D011247:Pregnancy; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*
D012333:RNA, Messenger; D028341:Activins; D015124:8-Bromo Cyclic Adenosine Monophosphate; D007265:Inhibins; D012313:RNA; D000242:Cyclic AMP