L-glutamate inhibition of an inward potassium current in neonatal neurons from the nucleus of the solitary tract.
Neurons isolated from the nucleus of the solitary tract (NTS) of 1- to 4-day-old rats were cultured for a study of the glutamatergic responses in this region of the medulla. Whole cell currents were examined under voltage clamp after 6-14 days in culture. An inwardly rectifying potassium current was identified in 107/174 cells. The presence of this K+ current diminished with time in culture from greater than 80% of the cells at day 6 to less than 30% of the cells after day 10. The current was inhibited by L-glutamate (IC50 = 10 microM).
Department of Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, TX 77030.
M
M
Priddy
J A
JA
Drewe
D L
DL
Kunze
D000818:Animals; D000831:Animals, Newborn; D001464:Barium / Q000494:pharmacology; D002478:Cells, Cultured; D003864:Depression, Chemical; D005971:Glutamates / Q000494:pharmacology*; D018698:Glutamic Acid; D015640:Ion Channel Gating / Q000187:drug effects*; D008526:Medulla Oblongata / Q000166:cytology*; D009475:Neurons, Afferent / Q000187:drug effects* / Q000378:metabolism; D011188:Potassium / Q000378:metabolism*; D015221:Potassium Channels / Q000187:drug effects*; D051381:Rats; D014781:Viscera / Q000294:innervation
D005971:Glutamates; D015221:Potassium Channels; D001464:Barium; D018698:Glutamic Acid; D011188:Potassium