Molecular identification of a soybean protein kinase gene family by using PCR.
In this study we report identification of six members of a protein kinase gene family from soybean (Glycine max L.). Two fully degenerate oligonucleotide primers corresponding to two conserved motifs (DLK-PENV and GTHEYLAPE) in the catalytic domains of eukaryotic protein serine/threonine kinases were used in a polymerase chain reaction (PCR) to amplify soybean cDNA. Sequence analysis showed that 28 of the PCR sequences represented six different putative protein serine/threonine kinases. These results not only demonstrate that catalytic domains of protein kinases are highly conserved between plants and other eukaryotes but also suggest that there are multiple genes encoding protein kinases in plants.
Center for Agricultural Biotechnology, University of Maryland, College Park 20742-5815.
X H
XH
Feng
P J
PJ
Bottino
S D
SD
Kung
D000595:Amino Acid Sequence; D017343:Genes, Plant; D008969:Molecular Sequence Data; D005810:Multigene Family; D011061:Poly A / Q000235:genetics / Q000302:isolation & purification; D016133:Polymerase Chain Reaction / Q000379:methods; D011494:Protein Kinases / Q000235:genetics*; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012333:RNA, Messenger; D012689:Sequence Homology, Nucleic Acid; D013025:Glycine max / Q000201:enzymology / Q000235:genetics*
D012333:RNA, Messenger; D011061:Poly A; D012313:RNA; D011494:Protein Kinases