Kinetochore-staining of spermatid micronuclei: studies of mice treated with X-radiation or acrylamide.
The rodent spermatid micronucleus (MN) assay was used in conjunction with immunofluorescent techniques to distinguish kinetochores in MN following exposure of mice to X-radiation or acrylamide. After either treatment, modest increases in kinetochore-positive MN were observed. Spermatids which had been exposed during meiotic prophase to X-rays (400 cGy) had approximately 10-fold increases in MN compared to controls; up to 15% of the MN observed were kinetochore-positive. Following acrylamide treatment of meiotic prophase cells, there was a doubling of spermatid MN over baseline levels, approximately one-third of which were kinetochore-positive.
Genetic Toxicology Division, U.S. Environmental Protection Agency, Research Triangle Park, NC.
B W
BW
Collins
D R
DR
Howard
J W
JW
Allen
D020106:Acrylamide; D000178:Acrylamides / Q000494:pharmacology*; D000818:Animals; D002875:Chromosomes / Q000187:drug effects* / Q000528:radiation effects*; D005069:Evaluation Studies as Topic; D005455:Fluorescent Antibody Technique; D005720:Gamma Rays; D008297:Male; D008540:Meiosis; D051379:Mice; D008810:Mice, Inbred C57BL; D013087:Spermatids / Q000187:drug effects / Q000528:radiation effects / Q000648:ultrastructure*; D013194:Staining and Labeling
D000178:Acrylamides; D020106:Acrylamide