Localization of mouse parathyroid hormone-like peptide gene (Pthlh) to distal chromosome 6 using interspecific backcross mice and in situ hybridization.
The single copy parathyroid hormone-like peptide gene (Pthlh) was mapped to distal mouse chromosome 6 using genetic linkage analysis with a panel of DNA samples from interspecific backcross mice. In all 114 meiotic events examined, the Pthlh locus cosegregated with the locus for the Kirsten ras-2 gene (Kras-2) which was previously localized to distal mouse chromosome 6. In addition, Pthlh was localized to chromosome 6 band F-G and the mouse parathyroid hormone Pth was localized to chromosome 7 band F, by in situ hybridization. These studies confirm the previous localization of Pthlh to mouse chromosome 6 using somatic cell hybrids and show that the Pthlh/PTHLH locus is a part of a conserved linkage group between distal mouse chromosome 6 and the proximal segment of the short arm of human chromosome 12.
Department of Medicine, Duke University Medical Center, Durham, NC.
M F
MF
Seldin
M G
MG
Mattei
G N
GN
Hendy
D000818:Animals; D015139:Blotting, Southern; D003433:Crosses, Genetic; D011905:Genes, ras / Q000235:genetics; D008040:Genetic Linkage / Q000235:genetics; D051379:Mice; D009693:Nucleic Acid Hybridization; D010281:Parathyroid Hormone / Q000235:genetics*; D044162:Parathyroid Hormone-Related Protein; D012150:Polymorphism, Restriction Fragment Length; D011506:Proteins / Q000235:genetics*
D010281:Parathyroid Hormone; D044162:Parathyroid Hormone-Related Protein; D011506:Proteins