Antipeptide antibodies to the carboxy terminal and the DCCD binding region of the human mitochondrial ATP synthase beta-subunit.
Antibodies to defined epitopes on the human ATP synthase would provide a powerful tool in the definition of the subunit composition of the enzyme complex and in the characterization of any defect in its assembly in diseases associated with mitochondrial disorders. Antibodies have been thus raised against synthetic peptides, corresponding to two regions on the human ATP synthase beta-subunit: the C-terminal region, and a region which includes the two dicyclohexylcarbodiimide (DCCD)-reactive glutamic acid residues suggested to be involved in the enzyme catalytic activity. The antibodies to the C-terminal peptide reacted with the ATP synthase beta-subunit in ELISA, in Western immunoblotting and in immunohistochemical experiments, and had the ability to immunoprecipitate the enzyme complex. The antibodies to the DCCD-binding region peptide did not react to the ATP synthase beta-subunit in its native configuration, although reacted well under Western immunoblotting conditions.
Noer
A S
AS
Department of Biochemistry, Monash University, Clayton, Australia.
Marzuki
S
S
Allison
W S
WS
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000276:immunology*; D001666:Binding Sites, Antibody / Q000276:immunology*; D002417:Cattle; D000939:Epitopes / Q000276:immunology; D005455:Fluorescent Antibody Technique; D005971:Glutamates; D018698:Glutamic Acid; D006801:Humans; D008928:Mitochondria / Q000201:enzymology; D008930:Mitochondria, Liver / Q000201:enzymology; D008931:Mitochondria, Muscle / Q000201:enzymology; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology; D011233:Precipitin Tests; D006180:Proton-Translocating ATPases / Q000276:immunology*; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D013045:Species Specificity
D000906:Antibodies; D000939:Epitopes; D005971:Glutamates; D010455:Peptides; D018698:Glutamic Acid; D006180:Proton-Translocating ATPases