Discrimination of aneuploidogens from clastogens by C-banding, DNA and area measurements of micronuclei from mouse bone marrow.
Micronuclei (MN) obtained from mouse bone marrow cells, in vivo exposed to 3 typical clastogens (procarbazine, azathioprine, ethyl methanesulfonate) and 3 typical aneuploidogens (vinblastine, tubulazole, colchicine), were examined for C-band, area and DNA content. C-banding allows a clear discrimination between clastogens and aneuploidogens: the clastogens do not exceed 50% C-band-positive MN and the aneuploidogens all 3 produce 65-75% C-band-positive MN. Concerning the DNA content the percentages of MN containing more DNA than an average chromosome (chr) are lower than 12% for the clastogens and 38-60% for the aneuploidogens. As far as the area of the MN is concerned the percentages of MN which have a larger area than chr are lower than 23% for the clastogens and range from 47% to 71% for the aneuploidogens. Additionally 3 other mutagens were studied. Hydroquinone induces 43% C-band-positive MN with DNA content far below the content of chr; considering the area measurements, however, hydroquinone behaves as an aneuploidogen (65% of the MN are larger than chr). Mitomycin C lies between the clastogens and the aneuploidogens for all 3 criteria but 5-azacytidine is comparable to the model aneuploidogens.
Laboratorium voor Antropogenetica, Vrije Universiteit Brussel, Belgium.
P
P
Van Hummelen
A
A
Deleener
P
P
Vanparys
M
M
Kirsch-Volders
D000782:Aneuploidy; D000818:Animals; D001374:Azacitidine / Q000633:toxicity; D001379:Azathioprine / Q000633:toxicity; D001853:Bone Marrow / Q000187:drug effects / Q000648:ultrastructure; D002871:Chromosome Banding; D003078:Colchicine / Q000633:toxicity; D004247:DNA / Q000032:analysis / Q000187:drug effects; D004148:Dioxolanes / Q000633:toxicity; D005020:Ethyl Methanesulfonate / Q000633:toxicity; D005260:Female; D006873:Hydroquinones / Q000633:toxicity; D008297:Male; D051379:Mice; D015162:Micronucleus Tests; D016685:Mitomycin / Q000633:toxicity; D009153:Mutagens / Q000633:toxicity*; D011344:Procarbazine / Q000633:toxicity; D014747:Vinblastine / Q000633:toxicity
D004148:Dioxolanes; D006873:Hydroquinones; D009153:Mutagens; D011344:Procarbazine; D016685:Mitomycin; C040422:tubulazole; D014747:Vinblastine; D004247:DNA; D005020:Ethyl Methanesulfonate; D001374:Azacitidine; D001379:Azathioprine; D003078:Colchicine; C031927:hydroquinone