Identification of an equivalent to murine Thy-1+ dendritic epidermal cells in the rat epidermis.
In the murine epidermis, there exist Thy-1+ dendritic epidermal cells (Thy-1+DEC). These cells are Thy-1+, CD45+, CD3+ and asialo GM1+ but CD5-, CD4-, CD8-, or Ia-1-, and express T cell receptor (TCR) gamma delta. Recently, most of these TCR gamma delta of Thy-1 DEC are shown to consist of a V gamma 3-V delta 1 combination. There has been no evidence that the same type of cell population exists in other species except mice. In this study, we investigated the existence of a Thy-1+DEC equivalent in the rat epidermis. The epidermal sheets obtained from rats were stained with various monoclonal antibodies to rat lymphocytes. We developed a monoclonal antibody (1F4) to rat CD3 complex. 1F4 stained thymocytes and peripheral T cells and also immunoprecipitated T cell receptor with CD3 complex. Using 1F4 and a recently developed monoclonal antibody to rat TCR alpha beta, we could identify dendritic CD4-, CD8-, CD5-, CD3+, TCR alpha beta- cells in the rat epidermis. These CD3+, TCR alpha beta- cells are strong candidates as an equivalent to TCR gamma delta + murine Thy-1+ DEC.
Shimada
S
S
Department of Dermatology, Yamanashi Medical College, Sendai, Japan.
Shibagaki
N
N
Tanaka
T
T
Kubota
Y
Y
Hashimoto
Y
Y
Tamaki
K
K
D000818:Animals; D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000032:analysis; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis; D000954:Antigens, Surface / Q000032:analysis*; D017252:CD3 Complex; D003713:Dendritic Cells / Q000737:chemistry / Q000166:cytology / Q000276:immunology*; D000078404:Epidermal Cells; D004817:Epidermis / Q000737:chemistry / Q000276:immunology; D005260:Female; D006649:Histocompatibility Antigens / Q000032:analysis; D017493:Leukocyte Common Antigens; D008562:Membrane Glycoproteins / Q000032:analysis*; D011233:Precipitin Tests; D051381:Rats; D011916:Rats, Inbred F344; D011948:Receptors, Antigen, T-Cell / Q000032:analysis; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000032:analysis; D013601:T-Lymphocytes / Q000737:chemistry / Q000276:immunology / Q000648:ultrastructure; D018800:Thy-1 Antigens
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface; D017252:CD3 Complex; D006649:Histocompatibility Antigens; D008562:Membrane Glycoproteins; D011948:Receptors, Antigen, T-Cell; D016692:Receptors, Antigen, T-Cell, gamma-delta; D018800:Thy-1 Antigens; D017493:Leukocyte Common Antigens