A new animal model of non-insulin-dependent diabetes mellitus induced by the NDK25 variant of encephalomyocarditis virus.
Intraperitoneal inoculation with NDK25, a variant of Encephalomyocarditis (EMC) virus which has been newly cloned from the M variant of EMC virus (EMC-M), caused DBA/2 mice to develop noninsulin-dependent diabetes mellitus. The NDK25-infected mice demonstrated abnormal glucose tolerance from 1 to 3 weeks after inoculation. The infection resulted in mild insulitis and the destruction of pancreatic beta cells at 1 week after inoculation and led to a significant reduction in the insulin content of the pancreas, but there was no ketonuria. The insulin content of the pancreas was recovered considerably from 15 +/- 3 at 1 week to 95 +/- 16 micrograms/g pancreas at 12 weeks, although the latter value was still significantly lower than that of the control mice (P < 0.05). Under microscopy, mild and partial infiltration of mononuclear cells was observed in about half the pancreatic islets only 1 week after inoculation, and then disappeared. Thus, we believe we have established a new model of noninsulin-dependent diabetes mellitus using the NDK25 variant of EMC virus.
Utsugi
T
T
2nd Department of Internal Medicine, Gunma University School of Medicine, Maebashi, Japan.
Kanda
T
T
Tajima
Y
Y
Tomono
S
S
Suzuki
T
T
Murata
K
K
Dan
K
K
Seto
Y
Y
Kawazu
S
S
D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000382:microbiology* / Q000503:physiopathology; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000382:microbiology* / Q000503:physiopathology; D004686:Encephalomyocarditis virus; D018149:Glucose Intolerance / Q000097:blood / Q000382:microbiology / Q000503:physiopathology; D005951:Glucose Tolerance Test; D007328:Insulin / Q000032:analysis; D007515:Islets of Langerhans / Q000737:chemistry / Q000473:pathology; D008297:Male; D051379:Mice; D008811:Mice, Inbred DBA; D010179:Pancreas / Q000473:pathology; D013997:Time Factors
D001786:Blood Glucose; D007328:Insulin