Bar to normal UGA translation by the selenocysteine tRNA.
The selC gene product, tRNA(Sec), inserts selenocysteine at UGA (opal) codons in a specialized mRNA context. We have investigated the action of the tRNA at ordinary UGA codons, normally not translated, by changing the unusual structural features of tRNA(Sec). Sequences in the D arm, CCA arm and variable arm of the tRNA all contribute to the prohibition against translation of ordinary UGA codons. One multiple mutant is a moderately efficient serine-inserting UGA suppressor tRNA.
Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder 80309-0347.
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D001483:Base Sequence; D003062:Codon; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D016153:Genes, Suppressor; D008969:Molecular Sequence Data; D014176:Protein Biosynthesis; D012323:RNA Processing, Post-Transcriptional; D012329:RNA, Bacterial / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D012345:RNA, Transfer, Amino Acid-Specific / Q000235:genetics*
D003062:Codon; D004269:DNA, Bacterial; D012329:RNA, Bacterial; D012333:RNA, Messenger; D012345:RNA, Transfer, Amino Acid-Specific; C036928:tRNA, selenocysteine-