Progression of HIV-related disease is associated with HLA DQ and DR alleles defined by restriction fragment length polymorphisms.
A cohort of 139 hemophiliacs was typed for HLA D region genes by means of restriction fragment length polymorphisms (RFLPs) detected by HLA DQ and DR gene probes. Disease progression was studied in the 65 HIV antibody-positive patients, who were infected by contaminated clotting factor before 1985. Strong associations were found between disease progression in HIV-infected patients and allelic DNA fragments revealed by a DQ alpha cDNA probe. A 5.5 kb fragment was reduced in frequency and a 4.6 kb fragment increased in frequency (p less than 0.005) in the faster progressing group, as measured both by development of CDC Category IV clinical symptoms and CD4 number less than 200 x 10(6)/l. These results correlate with DR types deduced from the RFLP patterns revealed by DR beta and DQ alpha gene probes. A decrease in DR4 and an increase in both DR5 and the DR3 subtype found in the A1 B8 DR3 haplotype were associated with disease progression (p less than 0.05).
Donald
J A
JA
School of Biological Sciences, Macquarie University, Sydney, Australia.
Rudman
K
K
Cooper
D W
DW
Baumgart
K W
KW
Garsia
R J
RJ
Gatenby
P A
PA
Rickard
K A
KA
D000483:Alleles; D001778:Blood Coagulation Disorders / Q000150:complications / Q000235:genetics / Q000276:immunology; D015331:Cohort Studies; D004247:DNA / Q000235:genetics; D004198:Disease Susceptibility / Q000276:immunology; D005787:Gene Frequency; D005802:Genes, MHC Class II; D020022:Genetic Predisposition to Disease; D015658:HIV Infections / Q000150:complications / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D006683:HLA-DQ Antigens / Q000032:analysis* / Q000235:genetics; D006684:HLA-DR Antigens / Q000032:analysis* / Q000235:genetics; D006239:Haplotypes; D006801:Humans; D012150:Polymorphism, Restriction Fragment Length; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics; D012720:Severity of Illness Index
D006683:HLA-DQ Antigens; D006684:HLA-DR Antigens; D016693:Receptors, Antigen, T-Cell, alpha-beta; D004247:DNA