12 alpha-hydroxytestosterone. A hitherto unidentified testosterone metabolite produced by cytochrome P-450 2A2.
Several cytochrome P-450 enzymes are able to hydroxylate testosterone to many different metabolites, some of which remain to be identified. One hitherto unidentified metabolite represents a major metabolite of P-450 2A2, present in the liver of adult male rats, and a minor metabolite of several other cytochrome P-450 enzymes. Using the techniques of HPLC and GC/MS, we have obtained unequivocal evidence that this metabolite is 12 alpha-hydroxytestosterone.
Laboratory of Chemical Pharmacology, National Heart, Lung & Blood Institute, National Institutes of Health, Bethesda, MD 20892.
S J
SJ
Smith
K R
KR
Korzekwa
T
T
Aoyama
F J
FJ
Gonzalez
J F
JF
Darbyshire
K
K
Sugiyama
J R
JR
Gillette
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003577:Cytochrome P-450 Enzyme System / Q000502:physiology*; D008401:Gas Chromatography-Mass Spectrometry; D006900:Hydroxylation; D008297:Male; D051381:Rats; D013739:Testosterone / Q000378:metabolism*
D013739:Testosterone; D003577:Cytochrome P-450 Enzyme System