Human immunodeficiency virus type 1 envelope glycoprotein gp120-mediated killing of human haematopoietic progenitors (CD34+ cells).
The effects of human immunodeficiency virus type 1 (HIV-1) and recombinant envelope glycoprotein gp120 on the in vitro growth of enriched human haematopoietic progenitors (CD34+ cells) have been investigated. A 2 h exposure to HIV-1 resulted in a progressive and significant reduction of viable CD34+ cell number in liquid cultures and of granulocyte-macrophage, erythroid and megakaryocytic progenitors in semisolid cultures. In virus-treated CD34+ cells, no signs of active virus replication were observed and the possibility of latent infection was excluded by quantitative polymerase chain reaction. Recombinant HIV-1 envelope glycoprotein gp120 added to CD34+ cell cultures displayed a dose-dependent inhibitory activity on CD34+ cell viability. Neutralizing antibody against gp120 was able to block completely the inhibitory activity on CD34+ cells of either HIV-1 or recombinant gp120. These results demonstrate that HIV-1 envelope glycoprotein gp120 has a direct cytotoxic effect on CD34+ cells.
Zauli
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G
Institute of Microbiology, University of Bologna, Italy.
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MC
Furlini
G
G
Giovannini
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M
La Placa
M
M
D015703:Antigens, CD / Q000032:analysis*; D018952:Antigens, CD34; D001854:Bone Marrow Cells; D002455:Cell Division; D002469:Cell Separation; D002470:Cell Survival; D002478:Cells, Cultured; D004279:DNA, Viral / Q000032:analysis; D004306:Dose-Response Relationship, Immunologic; D015686:Gene Products, env / Q000235:genetics; D015683:Gene Products, gag / Q000235:genetics; D015699:HIV Envelope Protein gp120 / Q000276:immunology / Q000502:physiology*; D015497:HIV-1 / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D006683:HLA-DQ Antigens / Q000235:genetics; D006412:Hematopoietic Stem Cells / Q000276:immunology / Q000382:microbiology*; D006801:Humans; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000276:immunology / Q000502:physiology
D015703:Antigens, CD; D018952:Antigens, CD34; D004279:DNA, Viral; D015686:Gene Products, env; D015683:Gene Products, gag; D015699:HIV Envelope Protein gp120; D006683:HLA-DQ Antigens; D011994:Recombinant Proteins