Enhancement and alteration of bleomycin-catalyzed site-specific DNA cleavage by distamycin A and some minor groove binders.
The effects of compounds which bind in the DNA minor groove of A.T rich sequences, on bleomycin-catalyzed site-specific DNA cleavage were investigated by a DNA sequencing technique. Distamycin A enhanced bleomycin-catalyzed DNA cleavage in G.C rich sequences such as 5'-GGGGC-3' (under scoring; the cleaved nucleotide). The cleavage in such a sequence in the presence of distamycin A was greater than that in the absence of distamycin A by as much as about 100 times. Neither Hoechst 33258, 4',6-diamidino-2-phenylindole (DAPI) nor berenil caused extensive enhancement. The results suggest that the distamycin-induced conformational changes of DNA through interactions other than the DNA minor groove binding in A.T-rich sequences are specifically suitable for the bleomycin action.
Yamamoto
K
K
Department of Public Health, Faculty of Medicine, Kyoto University, Japan.
Kawanishi
S
S
D001483:Base Sequence; D006690:Bisbenzimidazole / Q000494:pharmacology; D001761:Bleomycin / Q000494:pharmacology*; D004247:DNA / Q000187:drug effects*; D004249:DNA Damage / Q000187:drug effects*; D004133:Diminazene / Q000031:analogs & derivatives / Q000494:pharmacology; D004214:Distamycins / Q000494:pharmacology*; D007211:Indoles / Q000494:pharmacology; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation
D004214:Distamycins; D007211:Indoles; D001761:Bleomycin; C007293:DAPI; C030000:stallimycin; D004247:DNA; C003915:diminazene aceturate; D006690:Bisbenzimidazole; D004133:Diminazene