Alterations of bone marrow sinus endothelium induced by ionizing irradiation: implications in the homing of intravenously transplanted marrow cells.
The endothelium of bone marrow sinuses is a continuous layer which is selective in its cellular transport. It is not known how selective and massive seeding of hemopoietic progenitor cells after intravenous transplantation of marrow cells occurs. We postulate that the conditioning irradiation could disrupt the endothelial barrier, thus permitting the "homing" of progenitor cells to occur. To demonstrate this phenomenon, we irradiated mice with doses ranging from 100-2000 cGy-total body and studied perfusion-fixed marrow by transmission electron microscopy. The major finding was sloughing and denudation of plasma membrane, particularly on the luminal side of endothelium. Membrane vesiculation was also frequently seen in this border. Moreover, dilatation of the perinuclear space and rough endoplasmic reticulum was commonplace and testified to instability and fragility of the membrane system. Focal cytoplasmic swelling of endothelium was seen reflecting increased permissiveness of the endothelial barrier. Endocytosis and phagocytosis were increased in the marrow; and the endothelium, normally quiescent with regard to phagocytosis, was now overtly phagocytic. A dipogenecity of the adventitial layer was increased as hemopoietic function of marrow decreased. The end result of membrane alterations in the endothelium was the appearance of discontinuities in these cells, which form the essential element of bone marrow-blood barrier. Consequent to these discontinuities, the permissiveness of the endothelial barrier was enhanced and those cellular elements, such as mature, nonreticulated erythrocytes that are normally confined to the vascular space, now appeared in large number in the hemopoietic compartment. With low doses, these findings were transient and repair set in by 1-2 weeks. With higher doses, total disruption of marrow-blood barrier occurred and the process did not seem to be repairable. We conclude that the conditioning irradiation before bone marrow transplantation is essential in disrupting the endothelial barrier and permitting large-scale entry of transplanted cells into the hemopoietic compartment.
Shirota
T
T
Department of Veterans Affairs Medical Center, Jackson 39216.
Tavassoli
M
M
D000818:Animals; D001853:Bone Marrow / Q000098:blood supply / Q000528:radiation effects* / Q000648:ultrastructure; D016465:Bone Marrow Purging; D016026:Bone Marrow Transplantation / Q000379:methods*; D002462:Cell Membrane / Q000528:radiation effects / Q000648:ultrastructure; D002465:Cell Movement; D004307:Dose-Response Relationship, Radiation; D004705:Endocytosis; D004721:Endoplasmic Reticulum / Q000528:radiation effects / Q000648:ultrastructure; D004730:Endothelium, Vascular / Q000528:radiation effects* / Q000648:ultrastructure; D005260:Female; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000502:physiology*; D007262:Infusions, Intravenous; D051379:Mice; D008810:Mice, Inbred C57BL; D010587:Phagocytosis; D011828:Radiation Chimera; D011833:Radiation Injuries, Experimental / Q000473:pathology*; D014916:Whole-Body Irradiation