Genus- and species-specific oligonucleotide probes derived from 16S rRNA for the identification of vagococci.
Synthetic oligonucleotide probes specific for vagococci were designed from 16S rRNA sequence data. Molecular hybridizations with PCR-amplified rDNA targets provided an unequivocal means of differentiating vagococci from related lactic acid bacteria (eg. Carnobacterium, Enterococcus, Lactococcus) and identification at the generic and species levels.
Department of Microbiology, AFRC Institute of Food Research, Shinfield, Reading, UK.
A M
AM
Williams
M D
MD
Collins
D001483:Base Sequence; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D017045:Lactococcus / Q000235:genetics / Q000302:isolation & purification*; D013294:Lactococcus lactis / Q000235:genetics / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes / Q000737:chemistry*; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000737:chemistry*; D013045:Species Specificity; D013289:Streptococcaceae / Q000235:genetics / Q000302:isolation & purification*; D013291:Streptococcus / Q000235:genetics / Q000302:isolation & purification
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D015345:Oligonucleotide Probes; D012336:RNA, Ribosomal, 16S