Purification and characterization of an agglutinin of the soft coral Sinularia species.
A D-galactose-specific agglutinin, named sinularian, has been isolated from the soft coral Sinularia sp. by affinity chromatography on acid-treated Sepharose 4B and by gel filtration on HPLC. Sinularian was a glycoprotein containing 11% sugar. It gave a single band corresponding to 78 kDa in SDS-PAGE, irrespective of a treatment with 2-mercaptoethanol. Sinularian agglutinated rabbit erythrocytes and murine leukemia cells but not sheep or human ABO erythrocytes. Its hemagglutinating activity was Ca(++)-independent. Sinularian promoted binding of macrophages to tumor cells.
School of Fisheries Sciences, Kitasato University, Iwate, Japan.
R
R
Goto
K
K
Muramoto
M
M
Yamazaki
H
H
Kamiya
D000373:Agglutinins / Q000302:isolation & purification* / Q000378:metabolism; D000818:Animals; D002241:Carbohydrates / Q000494:pharmacology; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D003063:Cnidaria / Q000737:chemistry*; D004912:Erythrocytes / Q000187:drug effects; D006386:Hemagglutination Tests; D006801:Humans; D007942:Leukemia, Experimental / Q000473:pathology; D051379:Mice; D011817:Rabbits; D013045:Species Specificity; D014407:Tumor Cells, Cultured / Q000187:drug effects
D000373:Agglutinins; D002241:Carbohydrates