DNA damage induced by hypocrellin-A photosensitization.
Hypocrellin-A (HC-A) isolated from Hypocrellia bambusae Sacc., is a new and effective photosensitizer. Illumination of sarcoma 180 cells with visible light in the presence of HC-A leads to a decrease in cell viability and 3H-TdR incorporation, causes DNA strand breakage, and results in the selective destruction of guanine moieties in DNA. HC-A photosensitization causes an increase in the theta 260/theta 280 ratio in the circular dichroism spectra of DNA in vitro. Of the four usual 2'-deoxynucleotides illuminated in the presence of HC-A only 2'-deoxyguanylic acid was destroyed.
Institute of Biophysics, Academia Sinica, Beijing, People's Republic of China.
E H
EH
Cao
S M
SM
Xin
L S
LS
Cheng
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D004249:DNA Damage; D004277:DNA, Single-Stranded / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D010569:Perylene / Q000031:analogs & derivatives* / Q000494:pharmacology; D019800:Phenol; D010782:Photolysis; D011809:Quinones / Q000494:pharmacology*; D011838:Radiation-Sensitizing Agents / Q000494:pharmacology*
D004277:DNA, Single-Stranded; D004365:Drugs, Chinese Herbal; D011809:Quinones; D011838:Radiation-Sensitizing Agents; D019800:Phenol; D010569:Perylene; C048388:hypocrellin A