Electroporation-induced transformation of Escherichia coli: evaluation of a square waveform pulse.
Four strains of Escherichia coli were tested for electroporation-induced plasmid transformation using a high voltage pulse in a square waveform. Conditions for optimal transformation were determined for each strain and transformation frequencies found to be comparable to those reported previously for E. coli with a sinusoidal waveform of exponential decaying pulse.
Elvin
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Division of Biologics, PHLS Centre for Applied Microbiology and Research, Salisbury, Wiltsire, UK.
Bingham
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AH
D004558:Electric Stimulation; D004926:Escherichia coli / Q000235:genetics*; D010957:Plasmids; D014170:Transformation, Genetic