Radiation-induced chromosome aberrations analysed by fluorescence in situ hybridization with a triple combination of composite whole chromosome-specific DNA probes.
Fluorescence in situ hybridization (FISH) with a combination of three composite whole chromosome-specific DNA probes for human chromosomes 1, 4 and 12 was used to analyse in vitro radiation-induced dicentrics and symmetrical translocations in peripheral lymphocytes. Translocations could be rapidly and efficiently detected by FISH. Their frequencies were 1.8-fold higher than the frequencies for dicentrics at a given dose. The dose-response curves for translocations and dicentrics were linear quadratic with a significant higher quadratic component for translocations. The application of FISH for scoring stable translocations for biological dosimetry of radiation exposures is discussed.
Institut für Strahlenbiologie, GSF-Forschungszentrum für Umwelt und Gesundheit, Neuherberg, Germany.
E
E
Schmid
H
H
Zitzelsberger
H
H
Braselmann
J W
JW
Gray
M
M
Bauchinger
D000328:Adult; D001769:Blood / Q000528:radiation effects*; D002869:Chromosome Aberrations; D002878:Chromosomes, Human, Pair 1 / Q000528:radiation effects*; D002881:Chromosomes, Human, Pair 12 / Q000528:radiation effects*; D002894:Chromosomes, Human, Pair 4 / Q000528:radiation effects*; D015342:DNA Probes; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D066298:In Vitro Techniques; D008297:Male; D008875:Middle Aged; D011831:Radiation Genetics
D015342:DNA Probes