Production of monoclonal antibodies to Pseudomonas syringae pv. phaseolicola and Xanthomonas campestris pv. phaseoli.
The production of monoclonal antibodies (MAbs) to ethylenediamine tetraacetic acid (sodium salt) soluble antigens of Pseudomonas syringae pv. phaseolicola and Xanthomonas campestris pv. phaseoli (fuscans strain) is described. MAbs A6-1 and A6-2 produced to Ps. syringae pv. phaseolicola are pathovar specific. Although MAb XP2 produced to X. campestris pv. phaseoli recognized surface antigens of all strains of this pathovar (including fuscans strains) it cross-reacted specifically with X. campestris pv. malvacearum; it did not react with any other known bacteria or unidentified epiphytes from navy bean seed or leaves. The isotype of both MAbs XP2 and A6-1 is IgG3 whereas that of MAb A6-2 is IgG2a. The reactive antigens are thermostable, but their chemical nature has not been determined.
Department of Primary Industries, Plant Pathology Branch, Indooroopilly, Queensland, Australia.
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D000907:Antibodies, Bacterial / Q000096:biosynthesis* / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology; D000942:Antigens, Bacterial / Q000032:analysis / Q000276:immunology; D001419:Bacteria / Q000276:immunology; D004492:Edetic Acid; D004797:Enzyme-Linked Immunosorbent Assay; D007887:Fabaceae / Q000382:microbiology; D006825:Hybridomas; D007124:Immunoenzyme Techniques; D008070:Lipopolysaccharides / Q000276:immunology; D010946:Plants, Medicinal; D011549:Pseudomonas / Q000276:immunology*; D012703:Serotyping
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D008070:Lipopolysaccharides; D004492:Edetic Acid