Characterization of peripheral blood CD8/11b cells in bone marrow transplant recipients. III. Subsets of CD8/11b cells differentially regulate immunoglobulin production.
Recovering bone marrow transplant (BMT) recipients have 20-70% circulating lymphocytes which co-express CD8 and CD11b (normals = 5-15%). These CD8/11b cells comprise at least two subpopulations distinguished by their expression of CD3. The CD3+ CD8/11b cells are T lymphocytes which exhibit in vitro suppressor activity (Ts); the CD3- CD8/11b cells express CD16 and are natural killer (NK) cells. In this study, we investigated whether such cells influenced circulating levels of IgA and IgM in 18 BMT recipients who each had greater than 30% circulating CD8/11b cells. We observed that in all patients whose CD8/11b cells were Ts lymphocytes (7/7) IgM and IgA levels were less than 10% of normal. Among those patients whose CD8/11b cells were NK cells (n = 11), two groups were distinguished. In one group (n = 5), less than 35% of patient NK cells expressed CD57 and serum levels of IgM and IgA were less than 10% of normal. In the second group (n = 6) greater than 60% of the NK cells expressed CD57 and serum levels of IgM and IgA were normal. In summary, our data indicate that BMT recipients have at least three distinct subsets of CD8/11b lymphocyte populations which may differentially regulate IgM and IgA production in vivo.
OCLS Immunology, Rush Presbyterian St Luke's Medical Center, School of Medicine, Chicago 60612.
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D000293:Adolescent; D000328:Adult; D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D016026:Bone Marrow Transplantation / Q000009:adverse effects / Q000276:immunology*; D017252:CD3 Complex; D018959:CD57 Antigens; D016827:CD8 Antigens; D002648:Child; D005260:Female; D006801:Humans; D007070:Immunoglobulin A / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D007136:Immunoglobulins / Q000096:biosynthesis*; D007694:Killer Cells, Natural / Q000276:immunology; D016131:Lymphocyte Subsets / Q000276:immunology*; D016177:Macrophage-1 Antigen; D008297:Male; D011948:Receptors, Antigen, T-Cell; D016511:Severe Combined Immunodeficiency / Q000209:etiology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology
D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D017252:CD3 Complex; D018959:CD57 Antigens; D016827:CD8 Antigens; D007070:Immunoglobulin A; D007075:Immunoglobulin M; D007136:Immunoglobulins; D016177:Macrophage-1 Antigen; D011948:Receptors, Antigen, T-Cell