Use of early baculovirus promoters for continuous expression and efficient processing of foreign gene products in stably transformed lepidopteran cells.
Baculoviruses are currently used as vectors for the transient high-level expression of foreign gene products in insect cells. In this study, we demonstrate that baculoviruses can also be made to continuously express a foreign gene product by using the promoter from IE1, an immediate early viral gene, to produce stably-transformed insect cells. This approach gave levels of foreign gene expression lower than those usually obtained with the lytic baculovirus expression vector system. Expression, however, was continuous and stable, and a complex human glycoprotein (tissue plasminogen activator) was processed more efficiently. We conclude that stable transformation is a feasible approach for baculovirus-mediated foreign gene expression in lepidopteran cells, particularly for products that are relatively poorly-expressed and/or processed in lytically infected cells.
Department of Entomology, Texas A&M University, College Station.
D L
DL
Jarvis
J A
JA
Fleming
G R
GR
Kovacs
M D
MD
Summers
L A
LA
Guarino
D000818:Animals; D016367:Baculoviridae / Q000235:genetics*; D002461:Cell Line, Transformed; D003001:Cloning, Molecular; D004352:Drug Resistance, Microbial / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D005814:Genes, Viral / Q000235:genetics; D005822:Genetic Vectors; D007915:Lepidoptera / Q000166:cytology / Q000235:genetics*; D010957:Plasmids; D011401:Promoter Regions, Genetic / Q000235:genetics*; D010959:Tissue Plasminogen Activator / Q000235:genetics; D014162:Transfection / Q000235:genetics; D014170:Transformation, Genetic; D001616:beta-Galactosidase / Q000235:genetics
D001616:beta-Galactosidase; D010959:Tissue Plasminogen Activator