The complete amino acid sequence of the major Kunitz trypsin inhibitor from the seeds of Prosopsis juliflora.
The major inhibitor of trypsin in seeds of Prosopsis juliflora was purified by precipitation with ammonium sulphate, ion-exchange column chromatography on DEAE- and CM-Sepharose and preparative reverse phase HPLC on a Vydac C-18 column. The protein inhibited trypsin in the stoichiometric ratio of 1:1, but had only weak activity against chymotrypsin and did not inhibit human salivary or porcine pancreatic alpha-amylases. SDS-PAGE indicated that the inhibitor has a Mr of ca 20,000, and IEF-PAGE showed that the pI is 8.8. The complete amino acid sequence was determined by automatic degradation, and by DABITC/PITC microsequence analysis of peptides obtained from enzyme digestions of the reduced and S-carboxymethylated protein with trypsin, chymotrypsin, elastase, the Glu-specific protease from S. aureus and the Lys-specific protease from Lysobacter enzymogenes. The inhibitor consisted of two polypeptide chains, of 137 residues (alpha chain) and 38 residues (beta chain) linked together by a single disulphide bond. The amino acid sequence of the protein exhibited homology with a number of Kunitz proteinase inhibitors from other legume seeds, the bifunctional subtilisin/alpha-amylase inhibitors from cereals and the taste-modifying protein miraculin.
Departamento de Bioquimica, Universidade Federal do Rio Grande do Norte, Natal, Brazil.
A N
AN
Negreiros
M M
MM
Carvalho
J
J
Xavier Filho
A
A
Blanco-Labra
P R
PR
Shewry
M
M
Richardson
D000595:Amino Acid Sequence; D008969:Molecular Sequence Data; D010944:Plants / Q000032:analysis; D012639:Seeds / Q000032:analysis; D012689:Sequence Homology, Nucleic Acid; D014360:Trypsin Inhibitor, Kunitz Soybean / Q000737:chemistry* / Q000302:isolation & purification
D014360:Trypsin Inhibitor, Kunitz Soybean