Soluble intercellular adhesion molecule 1 is released by human melanoma cells and is associated with tumor growth in nude mice.
We have studied the cytokine regulation of cell surface and soluble intercellular adhesion molecule 1 (ICAM-1) expression on the human melanoma cell line A375M. Unstimulated cells express ICAM-1 on their cell surface but do not secrete significant levels of soluble ICAM-1. Interleukin 1, interleukin 6, tumor necrosis factor, and gamma-interferon all increased cell surface expression of ICAM-1. Tumor necrosis factor, interleukin 1, and gamma-interferon also caused the release of soluble ICAM-1. The serum of melanoma patients has been reported to contain elevated levels of soluble ICAM-1; however, the source of this ICAM-1 is unclear. The serum from nude mice bearing s.c. human melanoma tumors was found to contain soluble human ICAM-1. ICAM-1 levels showed a positive correlation with tumor weight. The release of ICAM-1 from melanoma tumors, in response to host-derived cytokines, may have relevance to immune recognition of the tumor.
Mario Negri Institute for Pharmacological Research, Bergamo, Italy.
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Giavazzi
R G
RG
Chirivi
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A
Garofalo
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A
Rambaldi
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I
Hemingway
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Pigott
A J
AJ
Gearing
D000818:Animals; D015815:Cell Adhesion Molecules / Q000097:blood / Q000378:metabolism*; D006801:Humans; D018799:Intercellular Adhesion Molecule-1; D007371:Interferon-gamma / Q000494:pharmacology; D007375:Interleukin-1 / Q000494:pharmacology; D015850:Interleukin-6 / Q000494:pharmacology; D008545:Melanoma / Q000097:blood / Q000378:metabolism*; D051379:Mice; D008819:Mice, Nude; D014407:Tumor Cells, Cultured
D015815:Cell Adhesion Molecules; D007375:Interleukin-1; D015850:Interleukin-6; D018799:Intercellular Adhesion Molecule-1; D007371:Interferon-gamma