Comparison of Clostridium sordellii toxins HT and LT with toxins A and B of C. difficile.
Clostridium sordellii produces two toxins, designated HT (haemorrhagic toxin) and LT (lethal toxin), that are similar to toxins A and B of C. difficile. The physicochemical properties of toxins HT and A were remarkably similar. The specific biological activities of toxin HT were almost the same as those of toxin A, and their NH2-terminal sequences shared close homology. The properties of toxins LT and B were similar, as were their NH2-terminal sequences, but toxin B was much more cytotoxic than toxin LT. Immunodiffusion analysis with specific antibodies showed that although toxins B and LT shared major antigenic determinants, each had unique epitopes. The results suggest that toxins B and LT have diverged more than toxins A and HT. Immunoblotting with antibodies to the toxins of C. difficile showed that toxins HT and LT had common antigenic determinants.
Martinez
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Department of Anaerobic Microbiology, Virginia Polytechnic Institute and State University, Blacksburg 24061.
Wilkins
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TD
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D001426:Bacterial Proteins; D001427:Bacterial Toxins / Q000737:chemistry* / Q000276:immunology / Q000302:isolation & purification; D016360:Clostridioides difficile / Q000737:chemistry*; D003013:Clostridium / Q000737:chemistry*; D004768:Enterotoxins / Q000737:chemistry* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes; D006386:Hemagglutination Tests; D007122:Immunoelectrophoresis; D008969:Molecular Sequence Data; D010449:Peptide Mapping
D000596:Amino Acids; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004768:Enterotoxins; D000939:Epitopes; C072293:hemorrhagic toxin HT, Clostridium sordellii; C072292:lethal toxin LT, Clostridium sordellii; C041348:tcdA protein, Clostridium difficile; C031815:toxB protein, Clostridium difficile