The micronucleus assay with mouse peripheral blood reticulocytes using acridine orange-coated slides with triethylenemelamine.
A micronucleus assay using mouse peripheral blood and supravital staining with acridine orange (AO) was validated by two laboratories on triethylenemelamine-treated mice. Dose- and time-dependent increases in micronucleated peripheral reticulocytes were observed. This new method can be used as an alternative to the conventional bone marrow micronucleus assay.
Yamamura
E
E
Toxicology Laboratories, Yoshitomi Pharmaceutical Industries, Ltd., Fukuoka-ken, Japan.
Hirono
H
H
Takeuchi
M
M
Kojima
M
M
Aoki
S
S
D000165:Acridine Orange; D000818:Animals; D004305:Dose-Response Relationship, Drug; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D015162:Micronucleus Tests / Q000379:methods; D016685:Mitomycin / Q000633:toxicity; D009153:Mutagens / Q000633:toxicity*; D012156:Reticulocytes / Q000187:drug effects*; D014265:Triethylenemelamine / Q000633:toxicity*
D009153:Mutagens; D016685:Mitomycin; D000165:Acridine Orange; D014265:Triethylenemelamine