Synergistic effects of interleukin 3 and interleukin 11 on murine megakaryopoiesis in serum-free culture.
We investigated the effects of interleukin 11 (IL-11) on murine megakaryopoiesis in serum-free cultures, using nonadherent, nonphagocytic, and T-cell-depleted bone marrow cells. IL-11 alone had no influence on megakaryocyte (Meg) colony formation in serum-free methylcellulose cultures, but it significantly enhanced the growth of Meg and granulocyte-macrophage-Meg colonies supported by optimal and suboptimal concentrations of interleukin 3 (IL-3). IL-11 also increased the size of IL-3-dependent Meg colonies as well as increasing the size and DNA content of constituent Meg. In liquid cultures, IL-11 alone did not increase the number of Meg, but it enhanced their size and acetylcholinesterase (AchE) levels. The addition of IL-11 to cultures containing suboptimal concentrations of IL-3 resulted in a synergistic increase of Meg AchE. These results suggest that IL-11, similarly to interleukin 6, has an effect on Meg and acts synergistically with IL-3 to augment murine megakaryopoiesis in vitro.
Yonemura
Y
Y
Second Department of Internal Medicine, Kumamoto University Medical School, Japan.
Kawakita
M
M
Masuda
T
T
Fujimoto
K
K
Kato
K
K
Takatsuki
K
K
D000110:Acetylcholinesterase / Q000032:analysis; D000818:Animals; D002452:Cell Count; D002478:Cells, Cultured; D016895:Culture Media, Serum-Free / Q000494:pharmacology*; D004247:DNA / Q000032:analysis; D004357:Drug Synergism; D005260:Female; D006410:Hematopoiesis / Q000187:drug effects*; D017370:Interleukin-11; D007377:Interleukin-3 / Q000494:pharmacology*; D007378:Interleukins / Q000494:pharmacology*; D008533:Megakaryocytes / Q000737:chemistry / Q000166:cytology* / Q000201:enzymology; D051379:Mice; D008810:Mice, Inbred C57BL; D011003:Ploidies
D016895:Culture Media, Serum-Free; D017370:Interleukin-11; D007377:Interleukin-3; D007378:Interleukins; D004247:DNA; D000110:Acetylcholinesterase