Boosted mucosal immune responsiveness in the rat intestine by actively transported hexose.
Anaphylaxis-mediated intestinal fluid secretion was measured in Trichinella spiralis- or ovalbumin-immunized rats challenged intraduodenally with T. spiralis somatic antigen (1 mg protein/0.5 mL saline) or ovalbumin (1 mg/0.5 mL saline), respectively. Intestinal fluid volume was measured 30 minutes after challenge as an index of net secretion. Challenge with the antigenic bolus containing 40 mmol/L D-glucose induced twice the fluid secretion as that induced by either antigen alone. L-Glucose was an ineffective substitute for D-glucose. The enhancement of secretion by D-glucose was dependent on prior immunologic sensitization, was diminished in the presence of phlorizin, and was mimicked by beta-methyl glucoside. Results indicate that the active transport of D-glucose augments the antigen-mediated fluid secretion, possibly by enhancing permeation of the intestinal epithelium to antigen, thereby providing greater access to the mucosal immune system.
Zhang
S
S
Department of Physiology and Cell Biology, University of Texas Medical School, Houston.
Castro
G A
GA
D000707:Anaphylaxis / Q000378:metabolism; D000818:Animals; D000941:Antigens / Q000276:immunology; D002712:Chlorides / Q000378:metabolism; D005947:Glucose / Q000494:pharmacology; D006601:Hexoses / Q000378:metabolism / Q000494:pharmacology*; D007109:Immunity / Q000187:drug effects*; D007114:Immunization; D007413:Intestinal Mucosa / Q000276:immunology*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D014234:Trichinella / Q000276:immunology
D000941:Antigens; D002712:Chlorides; D006601:Hexoses; D005947:Glucose