Simultaneous solid-phase extraction and chromatographic analysis of morphine and hydromorphone in plasma by high-performance liquid chromatography with electrochemical detection.
High-performance liquid chromatography has become an important analytical tool for the quantitation of opioid drugs. Using solid-phase extraction and coulometric electrochemical detection, we have developed a chromatographic method for the simultaneous measurement of morphine and hydromorphone which is both sensitive and specific. Using 1 ml of plasma, intra-assay and inter-assay data show that the detection limit for accurate quantitation of these compounds is about 1.2 ng/ml (coefficient of variation 11.6%) for morphine and 2.5 ng/ml (coefficient of variation 10.5%) for hydromorphone. The method is simple and readily adaptable to most pharmacokinetic studies and toxic screens involving these drugs.
Bouquillon
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AI
Department of Clinical Neurological Sciences, University of Western Ontario, Victoria Hospital, London, Canada.
Freeman
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Moulin
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DE
D002851:Chromatography, High Pressure Liquid; D004563:Electrochemistry; D006801:Humans; D004091:Hydromorphone / Q000097:blood* / Q000302:isolation & purification; D009020:Morphine / Q000097:blood* / Q000302:isolation & purification; D015203:Reproducibility of Results
D009020:Morphine; D004091:Hydromorphone