title: Heterogeneous expression of blood group A-determinant in a human gastric cancer cell line derived from a blood group A individual.
abstract: The human gastric cancer cell line MKN 45 was derived from the tumour of a blood group A individual, and was known to express large quantities of blood group A-antigen. Using immunofluorescence we found the MKN 45 cells, donated from the Japanese Cancer Research Resources Bank, consisted of A-antigen positive cells (18%) and A-antigen negative cells (82%). After limiting dilution, wild type and mutant cells were cloned with regard to the expression of a cell surface A-antigen. ELISA was used to detect A-antigen in the cell extract of the wild type cells, but none was evident in those of the mutant cells. However, blood group A-gene-specified transferase activity of the mutant cells was comparable to that of the wild type cells.
authors.affiliation: Department of Legal Medicine, Toyama Medical & Pharmaceutical University, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Kominato
authors.forename: T
authors.initials: T
authors.lastname: Fujikura
authors.forename: H
authors.initials: H
authors.lastname: Takizawa
authors.forename: K
authors.initials: K
authors.lastname: Hayashi
authors.forename: M
authors.initials: M
authors.lastname: Fujimaki
authors.forename: K
authors.initials: K
authors.lastname: Kishi
authors.forename: T
authors.initials: T
authors.lastname: Suzuki
mesh_terms: D000017:ABO Blood-Group System / Q000096:biosynthesis* / Q000235:genetics; D000939:Epitopes / Q000096:biosynthesis / Q000235:genetics; D005455:Fluorescent Antibody Technique; D015870:Gene Expression; D016695:Glycosyltransferases / Q000235:genetics; D006801:Humans; D011110:Polymorphism, Genetic; D013274:Stomach Neoplasms / Q000235:genetics / Q000276:immunology*; D014407:Tumor Cells, Cultured / Q000201:enzymology / Q000276:immunology
chemical_list: D000017:ABO Blood-Group System; D000939:Epitopes; D016695:Glycosyltransferases