Alpha 2-macroglobulin and other proteinase inhibitors do not interfere with the secretion of amyloid precursor protein in mouse neuroblastoma cells.
A series of proteinase inhibitors active against proteinases of all four major classes, including highly purified and well-characterized alpha 2-macroglobulin, added to the cell culture medium of murine Neuro 2a neuroblastoma cells did not interfere with APP secretase activity. We therefore advance the hypothesis that APP secretase activity is localized in an intracellular compartment.
De Strooper
B
B
Center for Human Genetics, University of Leuven, Belgium.
Van Leuven
F
F
Van den Berghe
H
H
D016564:Amyloid beta-Protein Precursor / Q000378:metabolism*; D000818:Animals; D015152:Blotting, Northern; D016521:Electrophoresis, Gel, Pulsed-Field; D051379:Mice; D009447:Neuroblastoma / Q000378:metabolism*; D011480:Protease Inhibitors / Q000378:metabolism*; D012313:RNA / Q000378:metabolism; D014407:Tumor Cells, Cultured; D000511:alpha-Macroglobulins / Q000378:metabolism*
D016564:Amyloid beta-Protein Precursor; D011480:Protease Inhibitors; D000511:alpha-Macroglobulins; D012313:RNA