title: Antibodies in normal and diseased pulps reactive with microorganisms isolated from deep caries.
abstract: Immunoglobulin molecules in the supernatant fluids (SF) from pulpal explant cultures have been observed to react with microorganisms implicated in infections of root canals. In this study, the reactivity of immunoglobulin molecules in the SF from normal and irreversible pulpitis pulps to six strains of predominant microorganisms isolated from the immediate layer of carious lesions above the pulps used for explant cultures was investigated using an enzyme-linked immunosorbent assay. Two ATCC strains of Eubacterium were also included in this assay. Specific antibodies to Lactobacillus casei subsp. casei, Lactobacillus casei subsp. rhamnosus, Lactobacillus acidophilus (I), (II), Streptococcus mutans, Bacteroides intermedius, Eubacterium brachy, and Eubacterium alactolyticum in the SF from the normal and irreversible pulpitis tissues were observed with a large variation of antibody levels in both groups. Immunodiffusion assays of the SF revealed that IgG was the major class of immunoglobulin in the normal as well as the irreversible groups. The presence of natural antibodies in the normal pulps suggested a possible protective role of antibodies during the invasive process of caries.
authors.affiliation: University of Missouri-Kansas City, School of Dentistry.
authors.forename: C L
authors.initials: CL
authors.lastname: Hahn
authors.forename: W A
authors.initials: WA
authors.lastname: Falkler
mesh_terms: D000907:Antibodies, Bacterial / Q000032:analysis*; D001439:Bacteroides / Q000276:immunology; D003731:Dental Caries / Q000276:immunology* / Q000382:microbiology; D003782:Dental Pulp / Q000276:immunology*; D003788:Dental Pulp Diseases / Q000276:immunology* / Q000382:microbiology; D004797:Enzyme-Linked Immunosorbent Assay; D005051:Eubacterium / Q000276:immunology; D006801:Humans; D005779:Immunodiffusion; D007070:Immunoglobulin A / Q000032:analysis; D007074:Immunoglobulin G / Q000032:analysis; D007778:Lactobacillus / Q000276:immunology; D013295:Streptococcus mutans / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D007070:Immunoglobulin A; D007074:Immunoglobulin G