title: Slow axonal transport of soluble proteins and calpain in retinal ganglion cells of aged rabbits.
abstract: The rate of slow axonal transport of soluble proteins in retinal ganglion cells of the rabbit decreased with approximately 25% in aged (6 years) compared to previous estimates in adult (2 years) animals. Immunobinding of calpain to microtiter plates coated with a monoclonal antibody to mu-calpain was used to isolate labelled axonally transported mu-calpain from the nerve extracts. It was found that the distribution of labelled mu-calpain in the retrobulbar optic pathway was similar to the distribution profile of the slowly migrating phase of soluble proteins.
authors.affiliation: Department of Biochemistry, Tokyo Metropolitan Institute of Gerontology, Japan.
authors.forename: J O
authors.initials: JO
authors.lastname: Karlsson
authors.forename: M
authors.initials: M
authors.lastname: Inomata
authors.forename: S
authors.initials: S
authors.lastname: Kawashima
mesh_terms: D000375:Aging / Q000502:physiology; D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D000911:Antibodies, Monoclonal; D001370:Axonal Transport / Q000502:physiology*; D015153:Blotting, Western; D002154:Calpain / Q000378:metabolism*; D005136:Eye Proteins / Q000378:metabolism*; D009900:Optic Nerve / Q000378:metabolism; D011817:Rabbits; D012165:Retinal Ganglion Cells / Q000378:metabolism*; D013462:Sulfur Radioisotopes; D014795:Visual Pathways / Q000166:cytology / Q000378:metabolism
chemical_list: D000596:Amino Acids; D000911:Antibodies, Monoclonal; D005136:Eye Proteins; D013462:Sulfur Radioisotopes; D002154:Calpain