title: Effect of growth temperature on outer membrane components and virulence of Aeromonas hydrophila strains of serotype O:34.
abstract: Growth of Aeromonas hydrophila strains from serotype O:34 at 20 and 37 degrees C in tryptic soy broth resulted in changes in the lipids, lipopolysaccharide (LPS), and virulence of the strains tested. Cells grown at 20 degrees C contained, relative to those cultured at 37 degrees C, increased levels of the phospholipid fatty acids hexadecanoate and octadecanoate and reduced levels of the corresponding saturated fatty acids. Furthermore, the lipid A fatty acids also showed thermoadaptation. In addition, LPS extracted from cells cultivated at 20 degrees C was smooth, while the LPS extracted from the same cells cultivated at 37 degrees C was rough. Finally, the strains were more virulent for fish and mice when they were grown at 20 degrees C than when they were grown at 37 degrees C and also showed increased different extracellular activities when they were grown at 20 degrees C.
authors.affiliation: Departamento de Microbiología, Universidad de Barcelona, Spain.
authors.forename: S
authors.initials: S
authors.lastname: Merino
authors.forename: S
authors.initials: S
authors.lastname: Camprubí
authors.forename: J M
authors.initials: JM
authors.lastname: Tomás
mesh_terms: D016980:Aeromonas hydrophila / Q000737:chemistry / Q000254:growth & development* / Q000472:pathogenicity; D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000032:analysis*; D005227:Fatty Acids / Q000032:analysis; D005260:Female; D006461:Hemolysis; D007928:Lethal Dose 50; D008070:Lipopolysaccharides / Q000032:analysis; D051379:Mice; D013696:Temperature; D014337:Trout; D014774:Virulence
chemical_list: D001425:Bacterial Outer Membrane Proteins; D005227:Fatty Acids; D008070:Lipopolysaccharides