Insulin-like growth-factor-binding protein gene expression and protein production by human tumour cell lines.
The secretion of insulin-like growth-factor-binding proteins (IGFBPs) and expression of the genes encoding IGFBP-1, IGFBP-2 and IGFBP-3 have been studied in a panel of cell lines derived from breast carcinomas, Wilms' tumour, neuroblastoma, retinoblastoma, colon carcinoma, liver adenocarcinoma, Burkitt's lymphoma and a non-small-cell lung carcinoma. All cell lines, with the exception of the Burkitt's lymphoma cell line, secreted IGFBPs, as detected by affinity labelling. A 34-kDa BP was present in the conditioned media of all IGFBP-secreting cell lines, whereas BPs ranging from 18 kDa to 53 kDa were variably secreted. All IGFBP-secreting cell lines expressed the IGFBP-2 gene as determined by Northern blot analysis. The Wilms' tumour, the neuroblastoma and the retinoblastoma cell line expressed the IGFBP-2 gene only. All other cell lines, with the exception of the Burkitt's lymphoma, expressed the IGFBP-2 gene and, in addition, either the IGFBP-1 gene and/or the IGFBP-3 gene. IGFBP-1 gene expression could be detected by reverse transcriptase polymerase chain reaction only. IGFBP-3 gene expression was detected by Northern blot analysis, but transcripts were less abundant than IGFBP-2 mRNAs. These findings indicate that the expression of multiple BP genes and the secretion of BPs may be a common property of tumour cells.
Reeve
J G
JG
Clinical Oncology and Radiotherapeutics Unit, MRC Centre, Cambridge, UK.
Kirby
L B
LB
Brinkman
A
A
Hughes
S A
SA
Schwander
J
J
Bleehen
N M
NM
D000345:Affinity Labels; D001483:Base Sequence; D015152:Blotting, Northern; D001943:Breast Neoplasms / Q000378:metabolism; D002352:Carrier Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D003110:Colonic Neoplasms / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D018969:Insulin-Like Growth Factor Binding Proteins; D008113:Liver Neoplasms / Q000378:metabolism; D008175:Lung Neoplasms / Q000378:metabolism; D008969:Molecular Sequence Data; D009369:Neoplasms / Q000378:metabolism*; D009447:Neuroblastoma / Q000378:metabolism; D016133:Polymerase Chain Reaction; D012175:Retinoblastoma / Q000378:metabolism; D014407:Tumor Cells, Cultured; D009396:Wilms Tumor / Q000378:metabolism
D000345:Affinity Labels; D002352:Carrier Proteins; D018969:Insulin-Like Growth Factor Binding Proteins