title: Plasma membrane inositol 1,4,5-trisphosphate-activated channels mediate signal transduction in lobster olfactory receptor neurons.
abstract: Inositol 1,4,5-trisphosphate (IP3) selectively evokes an inward (excitatory) current in cultured lobster olfactory receptor neurons (ORNs) and directly activates two types of channels in cell-free patches of plasma membrane from the ORNs. The IP3-activated channels have kinetic properties of odor-activated channels in the ORNs and pharmacological properties of intracellular IP3-activated channels in other systems. An antibody directed against an intracellular, cerebellar IP3 receptor recognizes a protein with a molecular weight similar to the mammalian receptor in the ORNs. The antibody selectively increases odor-evoked inward currents and IP3-activated unitary currents in the ORNs. The data provide further evidence for IP3 as an olfactory second messenger and implicate at least one and possibly two novel plasma membrane IP3 receptors in olfactory transduction.
authors.affiliation: Whitney Laboratory, University of Florida, St. Augustine 32086.
authors.forename: D A
authors.initials: DA
authors.lastname: Fadool
authors.forename: B W
authors.initials: BW
authors.lastname: Ache
mesh_terms: D000818:Animals; D000906:Antibodies; D015220:Calcium Channels; D002462:Cell Membrane / Q000502:physiology; D004553:Electric Conductivity; D015544:Inositol 1,4,5-Trisphosphate / Q000494:pharmacology*; D053496:Inositol 1,4,5-Trisphosphate Receptors; D007473:Ion Channels / Q000187:drug effects / Q000502:physiology*; D051379:Mice; D008121:Nephropidae / Q000502:physiology*; D009474:Neurons / Q000502:physiology*; D009812:Odorants; D011956:Receptors, Cell Surface / Q000276:immunology; D018160:Receptors, Cytoplasmic and Nuclear; D015290:Second Messenger Systems / Q000502:physiology; D011984:Sensory Receptor Cells / Q000502:physiology*; D015398:Signal Transduction / Q000502:physiology*; D012903:Smell / Q000502:physiology*
chemical_list: D000906:Antibodies; D015220:Calcium Channels; D053496:Inositol 1,4,5-Trisphosphate Receptors; D007473:Ion Channels; D011956:Receptors, Cell Surface; D018160:Receptors, Cytoplasmic and Nuclear; D015544:Inositol 1,4,5-Trisphosphate