title: Variation in the biological function of envelope protein epitopes of yellow fever vaccine viruses detected with monoclonal antibodies.
abstract: Three different virus strains (17D-204, 17DD and the French neurotropic vaccine) have been used as live attenuated yellow fever (YF) vaccines and are manufactured in different centres around the world. The envelope proteins of these vaccine viruses were examined and compared using mouse monoclonal antibodies (MAbs) in haemagglutination inhibition (HAI) and neutralization (N) tests. The epitopes eliciting HAI and/or N were found to vary depending on the virus examined. Such variation was also found between vaccine viruses of the same strain manufactured in different centres. These data were confirmed by the use of mouse polyclonal antisera. On the basis of the MAb results in HAI tests a dendrogram of the similarity coefficients between the viruses was constructed and showed that the viruses could be placed into three major groups. Thus, it is concluded that YF vaccines manufactured in different centres are antigenically distinct as recognized by the mouse immune system.
authors.affiliation: Molecular Microbiology Group, School of Biological Sciences, University of Surrey, Guildford, U.K.
authors.forename: T N
authors.initials: TN
authors.lastname: Ledger
authors.forename: B K
authors.initials: BK
authors.lastname: Sil
authors.forename: M R
authors.initials: MR
authors.lastname: Wills
authors.forename: G
authors.initials: G
authors.lastname: Lewis
authors.forename: R M
authors.initials: RM
authors.lastname: Kinney
authors.forename: A D
authors.initials: AD
authors.lastname: Jennings
authors.forename: J R
authors.initials: JR
authors.lastname: Stephenson
authors.forename: A D
authors.initials: AD
authors.lastname: Barrett
mesh_terms: D000911:Antibodies, Monoclonal; D006385:Hemagglutination Inhibition Tests; D006386:Hemagglutination Tests; D009500:Neutralization Tests; D013045:Species Specificity; D014613:Vaccines, Attenuated / Q000276:immunology; D014759:Viral Envelope Proteins / Q000276:immunology*; D010948:Viral Plaque Assay; D014765:Viral Vaccines / Q000276:immunology*; D015005:Yellow fever virus / Q000276:immunology* / Q000302:isolation & purification
chemical_list: D000911:Antibodies, Monoclonal; D014613:Vaccines, Attenuated; D014759:Viral Envelope Proteins; D014765:Viral Vaccines