The down-regulation of albumin transcription during regeneration is due to the loss of HNF-1 and the D-site transcription factors.
Liver regeneration has served as an important in vivo model for studying the control of differentiation. The molecular mechanisms responsible for the negative regulation of the albumin promoter during regeneration have not been examined previously. Changes in the proteins interacting with the albumin promoter were characterized using nuclear extracts prepared from the livers of rats undergoing chemically induced regeneration. Reduction in the activities of both hepatocyte nuclear factor-1 (HNF-1) and factors binding to the D site are responsible for the loss of albumin transcription during regeneration. No evidence for the involvement of a transcriptional repressor in this process was found.
Mueller
C R
CR
Cancer Research Laboratories, Queen's University, Kingston, Ontario, Canada.
D000418:Albumins / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D001483:Base Sequence; D022762:CCAAT-Enhancer-Binding Proteins; D004247:DNA; D004268:DNA-Binding Proteins / Q000378:metabolism; D003850:Deoxyribonuclease I; D015536:Down-Regulation; D005786:Gene Expression Regulation; D051537:Hepatocyte Nuclear Factor 1; D051538:Hepatocyte Nuclear Factor 1-alpha; D051539:Hepatocyte Nuclear Factor 1-beta; D008115:Liver Regeneration / Q000235:genetics*; D008969:Molecular Sequence Data; D016296:Mutagenesis; D009687:Nuclear Proteins / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D014157:Transcription Factors / Q000378:metabolism*; D014158:Transcription, Genetic
D000418:Albumins; D022762:CCAAT-Enhancer-Binding Proteins; C485975:DBP protein, rat; D004268:DNA-Binding Proteins; D051538:Hepatocyte Nuclear Factor 1-alpha; C496489:Hnf1a protein, rat; D009687:Nuclear Proteins; D014157:Transcription Factors; D051537:Hepatocyte Nuclear Factor 1; D051539:Hepatocyte Nuclear Factor 1-beta; D004247:DNA; D003850:Deoxyribonuclease I