title: Monoclonal antibodies to the C4 region of human immunodeficiency virus type 1 gp120: use in topological analysis of a CD4 binding site.
abstract: We have raised antisera and monoclonal antibodies (MAbs) to the C4 region of HIV-1 gp120, using an antigen chimaera of poliovirus as immunogen. These MAbs and sera, together with MAbs to the same region raised by other methods, fall into three groups defined by their abilities to bind to recombinant gp120 and/or the immunogenic peptide. In some cases, the amino acids recognized by the MAbs have been identified by pep-scan and by solution phase peptide inhibition of binding to recombinant gp120. Our results indicate that the amino acids WQEVGKAMYA are exposed on the surface of recombinant gp120. Antibodies to these amino acids on recombinant gp120 compete for soluble CD4 binding in vitro, but only weakly neutralize HIV.
authors.affiliation: Chester Beatty Laboratory, Institute of Cancer Research, London, England.
authors.forename: J A
authors.initials: JA
authors.lastname: McKeating
authors.forename: J P
authors.initials: JP
authors.lastname: Moore
authors.forename: M
authors.initials: M
authors.lastname: Ferguson
authors.forename: H S
authors.initials: HS
authors.lastname: Marsden
authors.forename: S
authors.initials: S
authors.lastname: Graham
authors.forename: J W
authors.initials: JW
authors.lastname: Almond
authors.forename: D J
authors.initials: DJ
authors.lastname: Evans
authors.forename: R A
authors.initials: RA
authors.lastname: Weiss
mesh_terms: D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000378:metabolism; D000956:Antigens, Viral / Q000235:genetics / Q000276:immunology; D001483:Base Sequence; D001665:Binding Sites; D001667:Binding, Competitive; D015704:CD4 Antigens / Q000378:metabolism*; D004279:DNA, Viral; D000939:Epitopes / Q000276:immunology; D015699:HIV Envelope Protein gp120 / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism; D015497:HIV-1 / Q000276:immunology* / Q000378:metabolism; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D017955:Poliovirus / Q000235:genetics
chemical_list: D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D015704:CD4 Antigens; D004279:DNA, Viral; D000939:Epitopes; D015699:HIV Envelope Protein gp120