Relationship between gonadotrophin subunit gene expression, gonadotrophin-releasing hormone receptor content and pituitary and plasma gonadotrophin concentrations during the rebound release of FSH after treatment of ewes with bovine follicular fluid during the luteal phase of the cycle.
The modulation of FSH secretion at the beginning and middle of the follicular phase of the cycle represents the key event in the growth and selection of the preovulatory follicle. However, the mechanisms that operate within the pituitary gland to control the increased release of FSH and its subsequent inhibition in vivo remain unclear. Treatment of ewes with bovine follicular fluid (bFF) during the luteal phase has been previously shown to suppress the plasma concentrations of FSH and, following cessation of treatment on day 11, a rebound release of FSH occurs on days 12 and 13. When luteal regression is induced on day 12, this hypersecretion of FSH results in an increase in follicle growth and ovulation rate. To investigate the mechanisms involved in the control of FSH secretion, ewes were treated with twice daily s.c. injections of 5 ml bFF on days 3-11 of the oestrous cycle and luteal regression was induced on day 12 with prostaglandin (PG). The treated ewes and their controls were then killed on day 11 (luteal), or 16 or 32 h after PG and their pituitaries removed and halved. One half was analysed for gonadotrophin and gonadotrophin-releasing hormone (GnRH) receptor content. Total pituitary RNA was extracted from the other half and subjected to Northern analysis using probes for FSH-beta, LH-beta and common alpha subunit. Frequent blood samples were taken and assayed for gonadotrophins. FSH secretion was significantly (P less than 0.01) reduced during bFF treatment throughout the luteal phase and then significantly (P less than 0.01) increased after cessation of treatment, with maximum secretion being reached 18-22h after PG, and then declining towards control values by 32h after PG. A similar pattern of LH secretion was seen after bFF treatment. Pituitary FSH content was significantly (P less than 0.05) reduced by bFF treatment at all stages of the cycle. No difference in the pituitary LH content was seen. The increase in GnRH receptor content after PG in the controls was delayed in the treated animals. Analysis of pituitary mRNA levels revealed that bFF treatment significantly (P less than 0.01) reduced FSH-beta mRNA levels in the luteal phase. Increased levels of FSH-beta, LH-beta and alpha subunit mRNA were seen 16h after PG in the bFF-treated animals, at the time when FSH and LH secretion from the pituitary was near maximum.(ABSTRACT TRUNCATED AT 400 WORDS)
Brooks
J
J
MRC Reproductive Biology Unit, University of Edinburgh, Centre for Reproductive Biology.
Crow
W J
WJ
McNeilly
J R
JR
McNeilly
A S
AS
D000818:Animals; D015152:Blotting, Northern; D046508:Culture Techniques; D015342:DNA Probes; D004958:Estradiol / Q000096:biosynthesis; D004971:Estrus; D005260:Female; D005640:Follicle Stimulating Hormone / Q000096:biosynthesis / Q000097:blood / Q000378:metabolism*; D037201:Follicle Stimulating Hormone, beta Subunit; D015870:Gene Expression; D007987:Gonadotropin-Releasing Hormone / Q000378:metabolism; D007265:Inhibins / Q000494:pharmacology; D008183:Luteal Phase / Q000502:physiology*; D007986:Luteinizing Hormone / Q000097:blood / Q000378:metabolism; D003341:Luteolysis / Q000187:drug effects; D006080:Ovarian Follicle / Q000502:physiology*; D010902:Pituitary Gland / Q000378:metabolism*; D011453:Prostaglandins / Q000378:metabolism; D012313:RNA / Q000302:isolation & purification; D012333:RNA, Messenger / Q000378:metabolism; D011966:Receptors, LHRH / Q000378:metabolism*; D012756:Sheep; D013997:Time Factors
D015342:DNA Probes; D037201:Follicle Stimulating Hormone, beta Subunit; D011453:Prostaglandins; D012333:RNA, Messenger; D011966:Receptors, LHRH; D007987:Gonadotropin-Releasing Hormone; D004958:Estradiol; D007265:Inhibins; D012313:RNA; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone