Detection of allele-specific expression of N-RAS oncogenes in human leukaemia cells.
We report analysis of allele-specific expression of N-RAS transcripts in myeloid leukaemic cells and cell lines. Expression was assessed by an assay of reverse-transcription/PCR combined with differential hybridization with mutation-specific oligonucleotides. In cells from all patients with acute myeloid leukaemia (AML) and in myeloid cell lines HL-60 and THP-1, expression of both the wild-type allele and the abnormal allele altered by a point mutation could be detected, albeit not always at comparable levels. This might be due for instance to allelic exclusion. The assay described provides a means of analysing the degree of expression of dominant oncogenes.
Lübbert
M
M
Department of Haematology/Oncology, University of Freiburg Medical Centre, Germany.
Mertelsmann
R
R
Herrmann
F
F
D000208:Acute Disease; D000483:Alleles; D001483:Base Sequence; D004273:DNA, Neoplasm / Q000235:genetics; D015870:Gene Expression / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D011905:Genes, ras / Q000235:genetics*; D006801:Humans; D015151:Immunoblotting; D007951:Leukemia, Myeloid / Q000235:genetics*; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000032:analysis; D016133:Polymerase Chain Reaction; D014158:Transcription, Genetic
D004273:DNA, Neoplasm; D009841:Oligonucleotides