Detection of benzo[a]pyrene sulfate and glucuronide conjugates in cell culture medium by directly coupled microbore high-performance liquid chromatography-fast atom bombardment mass spectrometry.
An improved method is described for detecting glucuronide and sulfate conjugates of benzo[a]pyrene in medium from cell cultures treated with benzo[a]pyrene. This method is based on a microbore high-performance liquid chromatograph directly coupled to a high-resolution continuous-flow fast atom bombardment mass spectrometer. Sulfate and glucuronide conjugates, as well as some structural isomers of glucuronide conjugates, were fully separated by the reversed-phase microbore high-performance liquid chromatography conditions used in this study. Since the method does not rely on the use of radiolabeled materials, it may be used to detect conjugates of a wide variety of hydrocarbons. The high sensitivity and selectivity of the method were demonstrated by detecting conjugates in the media of cell cultures derived from mice, hamsters and humans.
Teffera
Y
Y
Department of Medicinal Chemistry and Pharmacognosy, Purdue University, West Lafayette, IN 47907.
Baird
W M
WM
Smith
D L
DL
D000818:Animals; D001564:Benzo(a)pyrene / Q000032:analysis*; D002460:Cell Line; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006224:Cricetinae; D003470:Culture Media / Q000737:chemistry; D005965:Glucuronates / Q000032:analysis*; D006801:Humans; D051379:Mice; D016339:Spectrometry, Mass, Fast Atom Bombardment / Q000379:methods*; D013431:Sulfates / Q000032:analysis*; D014407:Tumor Cells, Cultured
D003470:Culture Media; D005965:Glucuronates; D013431:Sulfates; D001564:Benzo(a)pyrene