[Comparative study of the structure and protein composition of spermatozoid centrioles from sturgeons and salmon].
The aim of the present work was to compare the structure and protein composition of centrioles from spermatozoa of sturgeon and salmon fishes. The total protein content of the extracted fractions was studied by Na-SDS electrophoresis. Proteins with molecular weights from 15 to 170 kDa were detected. In both cases the major protein of centrioles is a protein with a molecular weight equal to that of tubulin. A protein with the molecular weight corresponding to actin was also detected. In both cases the ATPase activity stimulated by Ca2+ and Mg2+ ions was revealed. Electron microscopic studies showed differences in the ultrastructure of centrioles from sturgeon and salmon spermatozoa.
Mitina
N A
NA
Ostrovskaia
M V
MV
Shteĭn-Margolina
V A
VA
D000251:Adenosine Triphosphatases / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002413:Cations, Divalent; D002502:Centrioles / Q000201:enzymology / Q000378:metabolism / Q000648:ultrastructure*; D004591:Electrophoresis, Polyacrylamide Gel; D004789:Enzyme Activation; D005399:Fishes; D008274:Magnesium / Q000378:metabolism; D008297:Male; D008854:Microscopy, Electron; D009687:Nuclear Proteins / Q000378:metabolism*; D012474:Salmon; D013094:Spermatozoa / Q000201:enzymology / Q000378:metabolism*
D002413:Cations, Divalent; D009687:Nuclear Proteins; D000251:Adenosine Triphosphatases; D008274:Magnesium; D002118:Calcium