A technique for embedding undecalcified bone samples for detecting alpha-emitters using vacuum impregnation with Spurr's resin.
A method has been developed by which large samples of mineralized bone, containing an alpha-emitter, can be embedded in Spurr's resin in a fraction of the time required by conventional methods. Bone samples were freeze-dried or fixed and dried prior to impregnation with Spurr's resin under vacuum. Sections were cut for the preparation of either alpha-track or fission-track autoradiographs using the solid state detector CR-39. This method is applicable to samples containing a mobile form of a radionuclide that may be translocated during the processes of fixation and dehydration of the specimen.
Haines
J W
JW
National Radiological Protection Board, Didcot, Oxon, United Kingdom.
D000512:Alpha Particles; D000818:Animals; D001842:Bone and Bones / Q000033:anatomy & histology / Q000737:chemistry*; D006168:Guinea Pigs; D006801:Humans; D051379:Mice; D010215:Papio; D011883:Radium / Q000032:analysis*; D051381:Rats; D012116:Resins, Plant; D013194:Staining and Labeling; D013552:Swine; D016610:Tissue Embedding / Q000379:methods*; D014618:Vacuum
D012116:Resins, Plant; D011883:Radium