Trisomy 5 and trisomy 7 are nonrandom aberrations in pigmented villonodular synovitis: confirmation of trisomy 7 in uncultured cells.
Pigmented villonodular synovitis (PVNS) is a proliferative lesion of disputed genesis. Recently, we reported trisomy 7 in short-term cultures of 1 PVNS. In the present report, we describe another specimen of PVNS in which 9 of 26 (35 percent) metaphase cells demonstrated trisomy 7 when analyzed after 3-15 days of tissue culture. In situ hybridization analysis, with a biotinylated probe to chromosome 7 alpha-satellite DNA, revealed trisomy 7 in 53 of 200 uncultured cells from this PVNS sample. Our findings indicate that trisomy 7 is a nonrandom aberration that arises in vivo in PVNS.
Fletcher
J A
JA
Department of Pediatric Oncology, Dana-Farber Cancer Institute, Boston, Massachusetts.
Henkle
C
C
Atkins
L
L
Rosenberg
A E
AE
Morton
C C
CC
D002895:Chromosomes, Human, Pair 5; D002897:Chromosomes, Human, Pair 7; D046508:Culture Techniques; D005260:Female; D006801:Humans; D008875:Middle Aged; D009030:Mosaicism; D009693:Nucleic Acid Hybridization; D013586:Synovitis, Pigmented Villonodular / Q000235:genetics*; D014314:Trisomy