Unknown G0/G1 cell subpopulation revealed by hydrochloric acid/acridine orange staining.
An unknown cell subpopulation was observed in mouse and rat thymus, spleen and bone marrow cells, as well as in human peripheral blood mononuclear cells (resting and stimulated by PHA) using equilibrium HCl/acridine orange staining. This subpopulation includes cells with decreased green and unchanged red fluorescence. The staining does not affect cells in S- and G2/M-phases. The mechanism and biological meaning of the effect await further investigation.
Vinogradov
A E
AE
Institute of Cytology, USSR Academy of Sciences, Leningrad.
D000165:Acridine Orange; D000818:Animals; D002478:Cells, Cultured; D004492:Edetic Acid; D005434:Flow Cytometry / Q000379:methods; D016193:G1 Phase / Q000502:physiology*; D006801:Humans; D006851:Hydrochloric Acid; D007963:Leukocytes, Mononuclear / Q000166:cytology; D051379:Mice; D051381:Rats; D016192:Resting Phase, Cell Cycle / Q000502:physiology*; D013154:Spleen / Q000166:cytology; D013194:Staining and Labeling / Q000379:methods; D013950:Thymus Gland / Q000166:cytology
D004492:Edetic Acid; D000165:Acridine Orange; D006851:Hydrochloric Acid