title: An in vivo model for the experimental selection of drugs able to prevent immune complex glomerulonephritis.
abstract: Polyclonal activation of lymphocytes and immune complex-mediated glomerular lesions were induced in C57Bl/6 mice by injecting bacterial lipopolysaccharide (LPS) twice a week for 2 weeks. The usefulness of such a model for in vivo evaluation of immunomodulatory and therapeutic effects of drugs, was investigated by treating mice with DIAM4, a cyclophosphazenic compound known to modulate polyclonal activation of lymphocytes and to prevent mouse lupus nephritis. Prevention of LPS-triggered lymphocyte polyclonal activation and glomerular lesions was observed in the DIAM4-treated mice. Such a model can be used conveniently to select compounds effective in the treatment of immune glomerulonephritis.
authors.affiliation: Laboratoire d'Immunopathologie Rénale et d'Immunopharmacologie, CJF INSERM 88.01, Faculté de Médecine Purpan, Toulouse, France.
authors.forename: S
authors.initials: S
authors.lastname: In
authors.forename: A D
authors.initials: AD
authors.lastname: Le Lann
authors.forename: F
authors.initials: F
authors.lastname: Oksman
authors.forename: E L
authors.initials: EL
authors.lastname: Fournié
authors.forename: J F
authors.initials: JF
authors.lastname: Labarre
authors.forename: H
authors.initials: H
authors.lastname: Benoist
authors.forename: G J
authors.initials: GJ
authors.lastname: Fournié
mesh_terms: D000818:Animals; D000974:Antibodies, Antinuclear / Q000032:analysis; D001388:Aziridines / Q000627:therapeutic use; D003176:Complement C3 / Q000378:metabolism; D004195:Disease Models, Animal; D005260:Female; D005921:Glomerulonephritis / Q000276:immunology / Q000517:prevention & control*; D007105:Immune Complex Diseases / Q000276:immunology / Q000517:prevention & control*; D008070:Lipopolysaccharides / Q000633:toxicity; D051379:Mice; D008810:Mice, Inbred C57BL; D009943:Organophosphorus Compounds / Q000627:therapeutic use
chemical_list: D000974:Antibodies, Antinuclear; D001388:Aziridines; D003176:Complement C3; D008070:Lipopolysaccharides; D009943:Organophosphorus Compounds; C050390:DIAM 4