[A modified staining method for examining Y chromatin].
A modified staining method for Y chromatin is reported here which proved to be rapid and efficient for sex differentiation diagnosis. Samples were fixed in methanol: acetic acid (3:1) solution and stained with dyes in McIlvaine buffer. Y chromatin in buccal smears from normal males could be clearly revealed by this method. With this modified method and X-chromatin examination, one testicular feminization was found during examination of 1342 athletes for the 11th Asian Games.
Gao
C
C
Institute of Basic Medical Sciences, Beijing.
D002610:Cheek; D005260:Female; D006801:Humans; D008297:Male; D009061:Mouth Mucosa / Q000166:cytology; D012728:Sex Chromatin; D012732:Sex Determination Analysis; D013194:Staining and Labeling / Q000379:methods; D014998:Y Chromosome