High-performance liquid chromatographic determination of navelbine in MO4 mouse fibrosarcoma cells and biological fluids.
A high-performance liquid chromatographic method is described for separating and determining navelbine and possible metabolites in plasma, cell culture medium and MO4 cells. Navelbine is extracted from these fluids by ion-pair extraction with sodium octylsulphate as the counter-ion at pH 3. The system uses a cyano column as the stationary phase and a mobile phase of acetonitrile-0.12 M phosphate buffer (pH 3) (60:40, v/v). Application of the method to a study of the pharmacokinetic behaviour of navelbine in MO4 mouse fibrosarcoma cells is reported.
Van Belle
S J
SJ
Oncologisch Centrum, Vrije Universiteit Brussel, Belgium.
De Smet
M
M
Monsaert
C
C
Geerts
F
F
Storme
G A
GA
Massart
D L
DL
D000818:Animals; D000970:Antineoplastic Agents / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D003470:Culture Media; D005354:Fibrosarcoma / Q000737:chemistry* / Q000378:metabolism; D007202:Indicators and Reagents; D051379:Mice; D008809:Mice, Inbred C3H; D013056:Spectrophotometry, Ultraviolet; D014407:Tumor Cells, Cultured; D014747:Vinblastine / Q000031:analogs & derivatives* / Q000032:analysis / Q000097:blood / Q000493:pharmacokinetics; D000077235:Vinorelbine
D000970:Antineoplastic Agents; D003470:Culture Media; D007202:Indicators and Reagents; D014747:Vinblastine; D000077235:Vinorelbine