Evidence for two distinct major protein components, PAR 1 and PAR 2, in the paraflagellar rod of Trypanosoma cruzi. Complete nucleotide sequence of PAR.
The previously identified major protein components of the paraflagellar rod in Trypanosoma cruzi, PAR 1 and PAR 2, were analyzed to determine if they are distinct proteins or different conformations of a single polypeptide as has been suggested for other trypanosomatids. Amino acid sequence analysis showed PAR 1 and PAR 2 to be two distinct polypeptides. Antibodies specific against either PAR 1 or PAR 2 were shown to each react with a distinct band in Western blots of paraflagellar isolates of T. cruzi and other trypanosomatids if rigorous protease inhibition was used. The PAR 2 message was isolated and characterized by Northern blot and nucleic acid sequence analysis. Preliminary analysis of the PAR 2 gene indicates that PAR 2 is a member of a multigene family with all members residing on a single chromosome.
Beard
C A
CA
Department of Molecular Biology and Biochemistry, University of California, Irvine 92717.
Saborio
J L
JL
Tewari
D
D
Krieglstein
K G
KG
Henschen
A H
AH
Manning
J E
JE
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002850:Chromatography, Gel; D016054:DNA, Protozoan; D004591:Electrophoresis, Polyacrylamide Gel; D005407:Flagella / Q000737:chemistry*; D017125:Genes, Protozoan; D015151:Immunoblotting; D008969:Molecular Sequence Data; D005810:Multigene Family; D015800:Protozoan Proteins / Q000235:genetics* / Q000302:isolation & purification; D012333:RNA, Messenger / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D014349:Trypanosoma cruzi / Q000737:chemistry* / Q000235:genetics
D016054:DNA, Protozoan; D015800:Protozoan Proteins; D012333:RNA, Messenger; C077477:PAR 2 protein, Trypanosoma cruzi