Reactivity of human serum antibody with lipopolysaccharide O 78 antigen from enterotoxigenic Escherichia coli.
Fifteen and five of 20 volunteers challenged with the enterotoxigenic Escherichia coli strain O 78.H11 showed a fourfold titre increase of serum ELISA antibody to the homologous O 78 and the heterologous O 8 lipopolysaccharide antigen, respectively. Sixty-three of 191 sera from 1- to 48-month-old German children showed serum antibody reactive with O 78 antigen, all but two of these O 78-positive sera also showed reactivity with at least one further O antigen. Only 14 of the O 78 reactive sera also showed antibody to heat-labile enterotoxin. In addition, soluble O 8 antigen could inhibit the binding of serum antibody to absorbed O 78 in 68% of the German children. Antibody reactive with O 78 antigen is thus not a reliable serological marker for enterotoxigenic E. coli infection in German children.
Brüssow
H
H
Nestlé Research Centre, Nestec Ltd., Lausanne, Switzerland.
Sidoti
J
J
D000328:Adult; D000907:Antibodies, Bacterial / Q000276:immunology; D000937:Antigen-Antibody Reactions / Q000276:immunology*; D002675:Child, Preschool; D003429:Cross Reactions; D004768:Enterotoxins / Q000276:immunology*; D004926:Escherichia coli / Q000276:immunology*; D006801:Humans; D007075:Immunoglobulin M / Q000276:immunology; D007223:Infant; D007231:Infant, Newborn; D008070:Lipopolysaccharides / Q000276:immunology*; D019081:O Antigens; D011135:Polysaccharides, Bacterial / Q000276:immunology*
D000907:Antibodies, Bacterial; D004768:Enterotoxins; D007075:Immunoglobulin M; D008070:Lipopolysaccharides; D019081:O Antigens; D011135:Polysaccharides, Bacterial