title: Evidence that recombination between reiterated sequences in the Bacillus subtilis chromosome does not occur via unequal crossing over.
abstract: An experimental system was designed to permit the detection of recombination events occurring via unequal crossing over between sister bacterial chromosomes in Bacillus subtilis. It exploits the fact that during spore development, genetic and metabolic cooperation occurs between two different cell types, only one of which survives. During the early stages of sporulation, the two chromosomes of the developing sporangiole lie in the same cell and recombination between them is possible, in principle. Internal duplications flanking a selectable antibiotic-resistance gene have been introduced into the spoIIIC, spoIVA and spoVJ genes, whose correct expression in the mother cell (non-surviving compartment) is necessary for completion of spore development. After incubation in a sporulation-inducing medium in the absence of selective pressure, these strains sporulate at a low frequency and up to 30% of the progeny are Spo-. They result from mosaic sporangioles, in which only the chromosome segregated into the mother cell compartment of the developing sporangiole contains a reconstituted spo gene. In mosaic sporangioles generated by unequal crossing over between sister bacterial chromosomes, the insertionally inactivated spo gene, segregated into the pre-spore compartment, would carry an extra copy of the duplication initially present. Analysis of the products of 124 independent recombination events giving rise to mosaic sporangioles provided no evidence for the occurrence of unequal crossing over.
authors.affiliation: Pliva Research Institute, Zagreb, Croatia.
authors.forename: S
authors.initials: S
authors.lastname: Stojanović
authors.forename: D
authors.initials: D
authors.lastname: Hranueli
authors.forename: M
authors.initials: M
authors.lastname: Young
mesh_terms: D001412:Bacillus subtilis / Q000235:genetics* / Q000502:physiology; D002876:Chromosomes, Bacterial; D003434:Crossing Over, Genetic; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics*; D015870:Gene Expression; D009693:Nucleic Acid Hybridization; D011995:Recombination, Genetic; D012091:Repetitive Sequences, Nucleic Acid; D013171:Spores, Bacterial / Q000235:genetics / Q000502:physiology
chemical_list: D004269:DNA, Bacterial