In vivo effects of cadmium on rat liver glucocorticoid receptor functional properties.
1. Cadmium (Cd2+) administered in vivo induced a 40% reduction of rat liver glucocorticoid receptor (GR) capacity and inhibition of glucocorticoid-receptor complexes binding to mouse mammary tumor virus (MMTV) DNA fragment containing GR consensus sequence. 2. The effect of Cd2+ on the GR binding activity can be reversed with DTT, suggesting Cd2+ interaction with thiol groups. 3. Cd(2+)-related GR modification seems to be mediated by Cd2+ binding to cytoplasmic components included in the regulation of the receptor function, although the direct binding of the metal to the receptor thiols could not be ruled out.
Dunderski
J
J
Department of Ecology, Sinisa Stanković Institute for Biological Research, Belgrade, Yugoslavia.
Stanosević
J
J
Ristić
B
B
Trajković
D
D
Matić
G
G
D000818:Animals; D001667:Binding, Competitive / Q000187:drug effects; D002104:Cadmium / Q000633:toxicity*; D002482:Cellulose / Q000378:metabolism; D002614:Chelating Agents; D004247:DNA / Q000378:metabolism*; D007700:Kinetics; D008099:Liver / Q000737:chemistry*; D008297:Male; D011137:Polystyrenes; D011145:Polyvinyls; D051381:Rats; D017208:Rats, Wistar; D011965:Receptors, Glucocorticoid / Q000187:drug effects*; D013329:Structure-Activity Relationship
D002614:Chelating Agents; D011137:Polystyrenes; D011145:Polyvinyls; D011965:Receptors, Glucocorticoid; D002104:Cadmium; C006960:chelex; D002482:Cellulose; D004247:DNA