title: Effect of prolactin on 2-deoxyglucose uptake in mouse mammary gland explants.
abstract: These studies were carried out to explore the possible effect of prolactin (PRL) on glucose uptake into culture mammary gland explants derived from 12- to 14-day pregnant mice. PRL was found to stimulate an increased rate of uptake of a nonmetabolized glucose analogue, 2-[3H]deoxyglucose, into cultured mammary tissues. The onset of this response was 16 h after the addition of PRL, and the response persisted for at least 24 h. A similar temporal response was observed when the PRL stimulation of [14C]glucose oxidation to 14CO2 was determined. The lowest PRL concentration that elicited a stimulation of 2-deoxyglucose uptake was 20 ng/ml, and a maximum response occurred with PRL at a concentration of 250 ng/ml. Ongoing protein synthesis appears to be essential for PRL to express its effect on 2-deoxyglucose transport since cyclohexamide, puromycin, and actinomycin D abolished the PRL response. It is also apparent that the PRL stimulation of 2-deoxyglucose involves activation of a specific carrier-mediated uptake transport system, since the rate of uptake of L-glucose into mouse mammary gland explants was unaffected by PRL.
authors.affiliation: Department of Physiology, Wayne State University School of Medicine, Detroit, Michigan 48201.
authors.forename: B J
authors.initials: BJ
authors.lastname: Peters
authors.forename: J A
authors.initials: JA
authors.lastname: Rillema
mesh_terms: D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D046508:Culture Techniques; D003847:Deoxyglucose / Q000037:antagonists & inhibitors / Q000235:genetics / Q000493:pharmacokinetics*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008321:Mammary Glands, Animal / Q000378:metabolism*; D051379:Mice; D009994:Osmolar Concentration; D011388:Prolactin / Q000494:pharmacology*; D012313:RNA / Q000037:antagonists & inhibitors; D013997:Time Factors
chemical_list: D012313:RNA; D011388:Prolactin; D003847:Deoxyglucose