Age-related variations in relative abundance of alternative spliced D2 receptor mRNAs in brain areas of two rat strains.
Age-related reduction in the steady-state levels of messenger RNA for D2(415) and D2(444), the alternatively spliced form of dopamine D2 receptors, was observed in different rat brain areas using the sensitive reverse transcription (RT)-polymerase chain reaction (PCR) technique. In both Sprague-Dawley and Wistar aged rats, the decrease was more pronounced in the D2(444) isoform mRNA thus leading to a changed ratio in striatum as well as in the hippocampus.
Della Vedova
F
F
Center of Neuropharmacology, University of Milan, Italy.
Fumagalli
F
F
Sacchetti
G
G
Racagni
G
G
Brunello
N
N
D000375:Aging; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000254:growth & development / Q000502:physiology*; D005091:Exons; D008297:Male; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012326:RNA Splicing; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D011954:Receptors, Dopamine / Q000235:genetics*; D017448:Receptors, Dopamine D2; D013045:Species Specificity
D009838:Oligodeoxyribonucleotides; D012333:RNA, Messenger; D011954:Receptors, Dopamine; D017448:Receptors, Dopamine D2; D012313:RNA