title: Proton resonance assignments and three-dimensional solution structure of the ragweed allergen Amb a V by nuclear magnetic resonance spectroscopy.
abstract: Essentially complete assignment of the proton resonances in the allergenic protein Amb a V has been made by analysis of two-dimensional NMR experiments. Conformational constraints were obtained in three forms: interproton distances derived from NOE cross-peak intensities of NOESY spectra, torsion angle constraints derived from J-coupling constants of COSY and PE-COSY spectra, and hydrogen bond constraints derived from hydrogen-exchange experiments. Conformations of Amb a V with low constraint violations were generated using dynamic simulated annealing in the program XPLOR. The refined structures are comprised of a C-terminal alpha-helix, a small segment of antiparallel beta-sheet, and several loops. A hydrophobic core exists at the interface of the alpha-helix and beta-sheet. The derived structure accounts for the several anomalous proton chemical shifts that are observed. The structure determined here for Amb a V is topologically similar to the structure determined previously for the homologous allergenic protein Amb t V [Metzler, W. J., Valentine, K., Roebber, M., Friedrichs, M. S., Marsh, D., & Mueller, L. (1992) Biochemistry 31, 5117-5127]; however, significant differences exist in the packing of side chains in the hydrophobic core of the molecules. Comparison of the detailed structural features of these two proteins will allow us to suggest surface substructures for the Amb V allergens that are likely to participate in B cell epitopes.
authors.affiliation: Bristol-Myers Squibb Pharmaceutical Research Institute, Princeton, New Jersey 08543-4000.
authors.forename: W J
authors.initials: WJ
authors.lastname: Metzler
authors.forename: K
authors.initials: K
authors.lastname: Valentine
authors.forename: M
authors.initials: M
authors.lastname: Roebber
authors.forename: D G
authors.initials: DG
authors.lastname: Marsh
authors.forename: L
authors.initials: L
authors.lastname: Mueller
mesh_terms: D000485:Allergens / Q000276:immunology / Q000648:ultrastructure*; D000595:Amino Acid Sequence; D052179:Antigens, Plant; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000648:ultrastructure*; D011058:Pollen / Q000276:immunology / Q000648:ultrastructure*; D011487:Protein Conformation; D012996:Solutions
chemical_list: D000485:Allergens; D052179:Antigens, Plant; D010940:Plant Proteins; D012996:Solutions; C069543:group 5 allergens, Ambrosia