A tau-related protein of 130 kDa is present in Alzheimer brain.
Two abnormal entities of 69 and 130 kDa, immunologically related to the microtubule-associated tau proteins, are present in the hippocampus and the frontal cortex of the Alzheimer brain, which contain a large number of neurofibrillary tangles (NFTs), but are absent in the cerebellum, which does not contain these structures. Epitope mapping with antibodies spanning domains present in the N-terminal, middle, and C-terminal tau sequence demonstrated that the 69- and 130-kDa entities belong to the tau family. Both the 69- and the 130-kDa proteins were found in an insoluble form and were the major tau species present in purified NFTs. A procedure was devised that allowed us to prepare from Alzheimer hippocampi two NFT fractions differing in size (20 and 3 microns), both of which contained the tau entities of 130 and 69 kDa.
Gache
Y
Y
INSERM U. 282-CNRS, Hôpital Henri Mondor, Créteil, France.
Guilleminot
J
J
Ricolfi
F
F
Theiss
G
G
Nunez
J
J
D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000378:metabolism / Q000473:pathology*; D000906:Antibodies / Q000276:immunology; D015153:Blotting, Western; D001923:Brain Chemistry; D002531:Cerebellum / Q000737:chemistry / Q000473:pathology / Q000648:ultrastructure; D000939:Epitopes / Q000276:immunology; D006624:Hippocampus / Q000737:chemistry / Q000473:pathology / Q000648:ultrastructure; D006801:Humans; D008875:Middle Aged; D008970:Molecular Weight; D016874:Neurofibrillary Tangles / Q000648:ultrastructure; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D016875:tau Proteins / Q000032:analysis* / Q000276:immunology / Q000378:metabolism
D000906:Antibodies; D000939:Epitopes; D016875:tau Proteins; D012967:Sodium Dodecyl Sulfate