Platelet adhesion to collagen-coated wells: analysis of this complex process and a comparison with the adhesion to matrigel-coated wells.
The mechanisms of platelet adhesion to collagen type III-coated wells and Matrigel-coated wells were analyzed. The adhesion of 51Cr-labeled platelets to collagen-coated wells showed a biphasic pattern. The early stage of adhesion was inhibited by antibodies against platelet glycoprotein(GP)s Ia/IIa and VI. The later stage of platelet adhesion was inhibited by an antibody against the GPIIb/IIIa complex and a concomitant release of 14C-labeled serotonin was observed. The percentage of adhered platelets was increased when a higher platelet concentration was added in the reaction medium. These results indicated that the adhesion assay of platelets to collagen-coated wells was composed of two reactions: the first one is the platelet-collagen interaction that depends on GPIa/IIa and GPVI on the platelet surface; and the second reaction is the platelet-platelet interaction, platelet aggregation, which depends on GPIIb/IIIa. The adhesion of platelets to Matrigel-coated wells was indicated to involve platelet-Matrigel interactions that were partly dependent on the laminin in the Matrigel solution.
Moroi
M
M
Department of Protein Biochemistry, Kurume University, Japan.
Okuma
M
M
Jung
S M
SM
D000911:Antibodies, Monoclonal; D001792:Blood Platelets / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D002448:Cell Adhesion / Q000187:drug effects; D002452:Cell Count; D002478:Cells, Cultured; D003094:Collagen; D004338:Drug Combinations; D006801:Humans; D007797:Laminin; D009994:Osmolar Concentration; D010974:Platelet Aggregation; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology; D010980:Platelet Membrane Glycoproteins / Q000276:immunology / Q000378:metabolism; D011509:Proteoglycans; D012701:Serotonin / Q000378:metabolism
D000911:Antibodies, Monoclonal; D004338:Drug Combinations; D007797:Laminin; D010975:Platelet Aggregation Inhibitors; D010980:Platelet Membrane Glycoproteins; D011509:Proteoglycans; C059485:matrigel; D012701:Serotonin; D003094:Collagen