Gold labelling of RNA in virus-induced mitochondrial vesicles in the sheep blowfly Lucilia cuprina.
Membranous vesicles are thought to be the replication site for viral RNA of many plant and animal viruses. A relatively rare site of virus-associated vesiculation is that of the mitochondrial outer membrane. In this study, virus-induced mitochondrial vesicles of the blowfly, Lucilia cuprina, were labelled with ribonuclease/gold and an antibody against double stranded RNA (anti-polyinosinic:polycytidylic acid). Both methods showed the presence of RNA in the vesicles thus indicating they may be a site for viral RNA replication in Lucilia.
Binnington
K
K
Division of Entomology, CSIRO, Canberra A.C.T., Australia.
Brooks
L
L
D000818:Animals; D004175:Diptera / Q000382:microbiology*; D006046:Gold; D007150:Immunohistochemistry; D008928:Mitochondria / Q000737:chemistry / Q000648:ultrastructure; D012313:RNA / Q000032:analysis; D012328:RNA Viruses / Q000737:chemistry* / Q000648:ultrastructure; D012756:Sheep
D012313:RNA; D006046:Gold