A novel nuclear protein which binds to G gamma and A gamma globin promoters and modulates hemoglobin synthesis in K562 cells.
Nuclear extract of human erythroleukemic cell line K562 contains a 70 kDa protein which is gradually reduced when cells are induced to express globin genes by 25 microM hemin. When globin synthesis was inhibited by cycloheximide (100 micrograms/ml) or Actinomycin D (1 microgram/ml), the disappearance of this protein was prevented. The 70 kDa nuclear protein exhibited strong binding to G gamma and A gamma globin promoters but not to beta-globin promoter. This suggests that this 70 kDa nuclear protein may be involved in the regulation of fetal globin gene expression.
Baliga
B S
BS
Molecular Biology Division, University of South Alabama College of Medicine, Mobile 36617.
Phillips
K
K
Aliabadi
Z
Z
Mankad
V N
VN
D001483:Base Sequence; D004268:DNA-Binding Proteins / Q000378:metabolism / Q000502:physiology*; D005319:Fetal Hemoglobin / Q000096:biosynthesis*; D005786:Gene Expression Regulation / Q000502:physiology; D005914:Globins / Q000235:genetics* / Q000378:metabolism; D006427:Hemin / Q000502:physiology; D006801:Humans; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000378:metabolism / Q000502:physiology*; D011401:Promoter Regions, Genetic / Q000502:physiology*; D014407:Tumor Cells, Cultured
D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D006427:Hemin; D005914:Globins; D005319:Fetal Hemoglobin