Particle bombardment-mediated transient expression of a Brazil nut methionine-rich albumin in bean (Phaseolus vulgaris L.).
Bean (Phaseolus vulgaris L.) mature embryos were transformed using biolistic methods with a plasmid containing 2S albumin and beta-glucuronidase structural sequences, both under the control of the 35S CaMV promoter. We have shown that chimaeric tissues could be obtained and that both structural sequences were expressed to similar levels.
Aragao
F J
FJ
Centro Nacional de Pesquisas de Recursos Geneticos e Biotecnologia, EMBRAPA, Brasilia, D.F., Brazil.
de Sa
M F
MF
Almeida
E R
ER
Gander
E S
ES
Rech
E L
EL
D055516:2S Albumins, Plant; D052179:Antigens, Plant; D015153:Blotting, Western; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D007887:Fabaceae / Q000235:genetics*; D009754:Nuts / Q000235:genetics* / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D010946:Plants, Medicinal; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism
D055516:2S Albumins, Plant; D052179:Antigens, Plant; D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; C067568:methionine-rich 2S protein, Helianthus