Isolation of a yeast gene encoding a protein homologous to the human Tat-binding protein TBP-1.
We have cloned a putative yeast homolog of the gene encoding the human Tat-binding protein, TBP-1. The gene termed TBPY encodes a 45,243-dalton protein displaying a heptad repeat of hydrophobic amino acids reminiscent of a leucine zipper. Secondary structure predictions suggest the possibility of formation of an amphipathic helix that could further be organized into a coiled-coil. Additionally, the protein product of TBPY shows amino acid signatures characteristic of a large family of RNA and DNA helicases. We propose that the hydrophobic region of yTBP-1 participates in self-dimerization or heterodimerization.
Goyer
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C
Department of Biochemistry, McGill University, Montreal, Quebec.
Lee
H S
HS
Malo
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D
Sonenberg
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N
D000074183:ATPases Associated with Diverse Cellular Activities; D001483:Base Sequence; D015152:Blotting, Northern; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000235:genetics*; D016521:Electrophoresis, Gel, Pulsed-Field; D005656:Fungal Proteins / Q000235:genetics*; D005800:Genes, Fungal; D015756:Genes, tat; D006801:Humans; D008969:Molecular Sequence Data; D046988:Proteasome Endopeptidase Complex; D012333:RNA, Messenger / Q000032:analysis; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D012333:RNA, Messenger; D046988:Proteasome Endopeptidase Complex; D000074183:ATPases Associated with Diverse Cellular Activities; C064757:PSMC3 protein, human