Inhibition of recA induction by the radioprotector 2-mercaptoethylamine.
Our earlier finding that the radioprotective action of 2-mercaptoethylamine (MEA) is counteracted by ascorbate suggests a biochemical mechanism of action, which is supported by observations that MEA is not radioprotective in Rec- E. coli strains. In this study we show that MEA inhibits the induction of the recA gene by UV- or gamma-irradiation or by nalidixic acid in Escherichia coli strain GE94, which contains a recA-lacZ fusion. This effect, which may be counteracted by cysteine, indicates that in general MEA inhibits the induction of SOS functions.
Näslund
M
M
Department of Radiobiology, Stockholm University, Sweden.
Kolman
A
A
Ehrenberg
L
L
D003543:Cysteamine / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D003545:Cysteine / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics / Q000528:radiation effects; D005720:Gamma Rays; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects* / Q000528:radiation effects; D009268:Nalidixic Acid / Q000494:pharmacology; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D011938:Rec A Recombinases / Q000096:biosynthesis* / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics; D014466:Ultraviolet Rays; D001616:beta-Galactosidase / Q000235:genetics
D011837:Radiation-Protective Agents; D011993:Recombinant Fusion Proteins; D009268:Nalidixic Acid; D003543:Cysteamine; D011938:Rec A Recombinases; D001616:beta-Galactosidase; D003545:Cysteine