Comparative binding study of aluminum and chromium to human transferrin. Effect of iron.
The characteristics of aluminum and chromium binding to apo-transferrin (apo-tf) have been investigated and compared. Both metal ions were taken up by human transferrin forming complexes with the maximum absorbances at 405 nm for chromium-transferrin (cr-tf) and 240 nm for aluminum-transferrin (Al-tf). In the presence of citric acid, chromium binding to transferrin is five times more than aluminum. The binding of aluminum or chromium to apo-transferrin was reduced by 18 and 22% in the presence of 200 ng/mL of iron. The binding of both metals to apo-tf appears to be pH dependent. In acidic pHs, less chromium and more aluminum binding occurred.
Moshtaghie
A A
AA
Department of Biochemistry, School of Pharmacy, Isfahan University of Medical Sciences, Islamic Republic of Iran.
Ani
M
M
Bazrafshan
M R
MR
D000535:Aluminum / Q000378:metabolism*; D001059:Apoproteins / Q000378:metabolism; D002857:Chromium / Q000378:metabolism* / Q000493:pharmacokinetics; D002951:Citrates / Q000494:pharmacology; D019343:Citric Acid; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007501:Iron / Q000378:metabolism / Q000494:pharmacology*; D010070:Oxalates / Q000494:pharmacology; D019815:Oxalic Acid; D011485:Protein Binding / Q000187:drug effects; D013053:Spectrophotometry; D014168:Transferrin / Q000378:metabolism* / Q000493:pharmacokinetics
D001059:Apoproteins; D002951:Citrates; D010070:Oxalates; D014168:Transferrin; C066781:aluminum-transferrin; C028284:apotransferrin; D002857:Chromium; D019343:Citric Acid; D019815:Oxalic Acid; D000535:Aluminum; D007501:Iron