Rapid purification and N-terminal sequencing of a potato tuber cyclic nucleotide binding phosphatase.
A high affinity cyclic nucleotide binding phosphatase was purified to homogeneity from potato tubers by a rapid procedure involving batchwise elution from carboxymethylcellulose and gel filtration. The phosphatase has a molecular weight of 28,000 as estimated from both SDS-PAGE and gel filtration. The phosphatase binds to Con A-agarose and is eluted by 0.5 M alpha-methylglucoside. The phosphatase catalyses the hydrolysis of nucleoside monophosphates, p-nitrophenylphosphate and O-phospho-L-tyrosine, but not of O-phospho-L-serine or O-phospho-L-threonine. N-terminal sequencing of the phosphatase has revealed significant homology with two similar-size soybean leaf and stem storage glycoproteins.
Polya
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GM
Department of Biochemistry, La Trobe University, Bundoora, Australia.
Wettenhall
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RE
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D006868:Hydrolysis; D008969:Molecular Sequence Data; D009596:Nitrophenols / Q000378:metabolism; D009712:Nucleotides, Cyclic / Q000378:metabolism*; D009943:Organophosphorus Compounds / Q000378:metabolism; D010744:Phosphoric Monoester Hydrolases / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D019000:Phosphotyrosine; D016415:Sequence Alignment; D011198:Solanum tuberosum / Q000201:enzymology*; D014443:Tyrosine / Q000031:analogs & derivatives / Q000378:metabolism
D000596:Amino Acids; D009596:Nitrophenols; D009712:Nucleotides, Cyclic; D009943:Organophosphorus Compounds; D019000:Phosphotyrosine; C008644:nitrophenylphosphate; D014443:Tyrosine; D010744:Phosphoric Monoester Hydrolases