Amino acids and peptides. XXXIII. Synthesis of N-terminal epitope peptides of mammalian metallothioneins (MTs).
In order to determine the fine structure of the mammalian metallothionein (MT) epitope to a monoclonal anti-rat Zn-MT-II antibody (MT 189-14-7), N-terminal peptides of various lengths of mammalian metallothioneins (MTs) were synthesized by a conventional solution method using the newly developed beta-2-adamantylaspartate, and their immunological properties were examined. It was found that the N-terminal acetyl group was indispensable for the reaction with the monoclonal antibody and the N-terminally acetylated pentapeptide, Ac-Met-Asp-Pro-Asn-Cys-OH, was the smallest peptide which exhibited a significant reactivity with the antibody.
Okada
Y
Y
Faculty of Pharmaceutical Sciences, Kobe-Gakuin University, Japan.
Nakayama
S
S
Iguchi
S
S
Kikuchi
Y
Y
Irie
M
M
Sawada
J
J
Ikebuchi
H
H
Terao
T
T
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000138:chemical synthesis*; D000818:Animals; D000939:Epitopes; D008668:Metallothionein / Q000138:chemical synthesis / Q000276:immunology*; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000138:chemical synthesis* / Q000276:immunology
D000596:Amino Acids; D000939:Epitopes; D010446:Peptide Fragments; D008668:Metallothionein