title: Group C rotavirus requires sialic acid for erythrocyte and cell receptor binding.
abstract: Current immunological and biochemical information regarding the hemagglutinin and virus-cell interactions of rotavirus is obtained exclusively from studies with group A rotaviruses. In this study, I report that the immunologically and genetically distinct group C rotavirus also possesses a hemagglutinin. The viral hemagglutinin was identified on a cultivable porcine group C rotavirus strain (strain AmC-1) by using agglutinated human and guinea pig erythrocytes. Neuraminidase treatment of fresh human erythrocytes or blocking with glycophorin A or fetuin prevented hemagglutination. Infection of swine testicular cells with group C AmC-1 virus was also prevented by glycophorin A, fetuin, and neuraminidase treatment, suggesting that sialic acid constitutes an essential part of the cell receptor.
authors.affiliation: Department of Virology, National Bacteriological Laboratory, Stockholm, Sweden.
authors.forename: L
authors.initials: L
authors.lastname: Svensson
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004912:Erythrocytes / Q000378:metabolism* / Q000382:microbiology; D006021:Glycophorins / Q000494:pharmacology; D006384:Hemagglutination / Q000187:drug effects; D019158:N-Acetylneuraminic Acid; D009439:Neuraminidase / Q000494:pharmacology; D011991:Receptors, Virus / Q000378:metabolism*; D012401:Rotavirus / Q000378:metabolism*; D012794:Sialic Acids / Q000378:metabolism*; D013552:Swine; D000509:alpha-Fetoproteins / Q000494:pharmacology
chemical_list: D006021:Glycophorins; D011991:Receptors, Virus; D012794:Sialic Acids; D000509:alpha-Fetoproteins; D009439:Neuraminidase; D019158:N-Acetylneuraminic Acid