title: Equine protozoal myelitis in Panamanian horses and isolation of Sarcocystis neurona.
abstract: Schizonts of Sarcocystis neurona were identified microscopically in hematoxylin-eosin-stained spinal cord sections from 2 native Panamanian horses that exhibited clinical signs of equine protozoal myelitis (EPM). Spinal cord homogenate from a third Panamanian horse with EPM was inoculated onto monolayers of cultured bovine monocytes (M617). Intracytoplasmic schizonts containing merozoites arranged in rosette forms surrounding a central residual body first were observed 13 wk postinoculation. Parasites divided by endopolygeny and lacked rhoptries. Schizonts from each horse reacted with Sarcocystis cruzi antiserum in an immunohistochemical test.
authors.affiliation: M.H. Gluck Equine Research Center, Department of Veterinary Science, University of Kentucky, Lexington 40546-0099.
authors.forename: D E
authors.initials: DE
authors.lastname: Granstrom
authors.forename: O
authors.initials: O
authors.lastname: Alvarez
authors.forename: J P
authors.initials: JP
authors.lastname: Dubey
authors.forename: P F
authors.initials: PF
authors.lastname: Comer
authors.forename: N M
authors.initials: NM
authors.lastname: Williams
mesh_terms: D000818:Animals; D002460:Cell Line; D005260:Female; D006734:Horse Diseases / Q000453:epidemiology / Q000469:parasitology* / Q000473:pathology; D006736:Horses; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D008297:Male; D008854:Microscopy, Electron; D009187:Myelitis / Q000453:epidemiology / Q000469:parasitology / Q000473:pathology / Q000662:veterinary*; D010176:Panama / Q000453:epidemiology; D012522:Sarcocystis / Q000302:isolation & purification* / Q000648:ultrastructure; D012523:Sarcocystosis / Q000453:epidemiology / Q000469:parasitology / Q000473:pathology / Q000662:veterinary*; D013116:Spinal Cord / Q000469:parasitology / Q000473:pathology