title: The application of aqueous two-phase systems to oligosaccharide synthesis by alpha-mannosidase catalysed glycosyl transfer reactions.
abstract: Aqueous two-phase systems formed from PEG and dextran have been applied to the synthesis of oligosaccharide by Jack bean alpha-mannosidase in reverse. Whilst rates of synthesis and percentage yields were similar in two-phase systems and one-phase aqueous buffer systems, a ten-fold increase in yield of product per unit of enzyme was seen. In addition, the use of aqueous two-phase systems offers potential process advantages over one-phase systems for the synthesis of oligosaccharides.
authors.affiliation: Division of Biotechnology, School of Biological and Health Sciences, University of Westminster, London, UK.
authors.forename: T J
authors.initials: TJ
authors.lastname: Bartlett
authors.forename: R A
authors.initials: RA
authors.lastname: Rastall
authors.forename: N H
authors.initials: NH
authors.lastname: Rees
authors.forename: M W
authors.initials: MW
authors.lastname: Adlard
authors.forename: C
authors.initials: C
authors.lastname: Bucke
mesh_terms: D002240:Carbohydrate Sequence; D003911:Dextrans / Q000737:chemistry; D005069:Evaluation Studies as Topic; D006027:Glycosides / Q000138:chemical synthesis; D007700:Kinetics; D008361:Mannosidases / Q000378:metabolism*; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000138:chemical synthesis*; D011092:Polyethylene Glycols / Q000737:chemistry; D012995:Solubility; D014867:Water / Q000737:chemistry; D043323:alpha-Mannosidase
chemical_list: D003911:Dextrans; D006027:Glycosides; D009844:Oligosaccharides; D014867:Water; D011092:Polyethylene Glycols; D008361:Mannosidases; D043323:alpha-Mannosidase