Microsomal ethanol-oxidizing system in Euglena gracilis. Similarities between Euglena and mammalian cell systems.
1. ADH activity of Euglena grown with 50 mM ethanol decreased, but MEOS activity increased with a corresponding increase in the total amount of cytochrome P-450. 2. Phenobarbital treatment increased the total amount of cytochrome P-450. 3. CO and KCN, cytochrome P-450 ligands, diminished acetaldehyde formed from ethanol oxidation by MEOS. 4. The amounts of NAD(P)H cytochrome c reductases and cytochrome b5 type, components of microsomal monooxygenase reaction, have been spectrophotometrically measured. 5. NAD(P)H cytochrome c reductases activities were induced by phenobarbital. 6. DMSO, an inhibitor of rabbit MEOS, inhibited O2 consumption (11-20%) by Euglena grown with an ethanol, but not a lactate medium. 7. These studies indicate the presence of cytochrome P-450-dependent MEOS in Euglena similar to that in the mammalian hepatic cell.
Julistiono
H
H
Laboratoire des Membranes Biologiques, Université Paris, France.
Briand
J
J
D000426:Alcohol Dehydrogenase / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D002478:Cells, Cultured; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis*; D015786:Cytochromes b5 / Q000378:metabolism; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D004790:Enzyme Induction / Q000187:drug effects; D000431:Ethanol / Q000378:metabolism / Q000494:pharmacology*; D005056:Euglena gracilis / Q000187:drug effects* / Q000378:metabolism; D008322:Mammals / Q000378:metabolism*; D008861:Microsomes / Q000187:drug effects* / Q000378:metabolism; D009251:NADPH-Ferrihemoprotein Reductase / Q000187:drug effects / Q000378:metabolism; D010084:Oxidation-Reduction; D010101:Oxygen Consumption / Q000187:drug effects; D010634:Phenobarbital / Q000494:pharmacology
D000431:Ethanol; D015786:Cytochromes b5; D003577:Cytochrome P-450 Enzyme System; D000426:Alcohol Dehydrogenase; D009251:NADPH-Ferrihemoprotein Reductase; D004121:Dimethyl Sulfoxide; D010634:Phenobarbital