[Morphology and immunotyping of AC-43 strain of Chlamydia pneumoniae isolated from a Japanese child].
A strain of Chlamydia pneumoniae (C. pneumoniae) which had been isolated from a Japanese child (AC-43) was examined morphologically and serologically using micro-immunofluorescent (micro-IF) test. Inclusions of AC-43 were stained by an indirect immunofluorescent method using C. pneumoniae specific monoclonal antibody. They were dense round inclusions which had been reported as a characteristic figure for C. pneumoniae. An elementary body (EB) of AC-43 was pear-shaped by electron micrograph, which was the same as previous reports for C. pneumoniae. We produced monoclonal antibodies using purified EB of AC-43 as antigen. Culture fluids of these clones reacted with C. pneumoniae antigens, but did not react with C. trachomatis or C. psittaci antigens by micro-IF tests. There was no difference in morphological and serological findings among standard strains and Japanese isolate of C. pneumoniae.
Yamazaki
T
T
Department of Pathobiology, University of Washington.
D000942:Antigens, Bacterial / Q000032:analysis; D002675:Child, Preschool; D002690:Chlamydia Infections / Q000382:microbiology*; D016993:Chlamydophila pneumoniae / Q000145:classification* / Q000302:isolation & purification / Q000648:ultrastructure; D005455:Fluorescent Antibody Technique; D006801:Humans; D007564:Japan; D008854:Microscopy, Electron; D012703:Serotyping
D000942:Antigens, Bacterial