Solubilization of ribosomes in reverse micelles.
Pig liver ribosomes have been solubilized in reverse micelles constituted by bis (2-ethyl hexyl) sodium sulfosuccinate (AOT) in isooctane and 3.6% water, v:v. The micellar ribosomal solutions are transparent, show no significant scattering and permit direct spectroscopic observation of the ribosomes to be made. Ultraviolet absorption and circular dichroic spectra have been recorded and indicate that the ribosomes maintain in the micellar environment their structural integrity. Some possible applications of these micellar systems are discussed.
Palazzo
G
G
Dipartimento di Chimica, Universitá di Bari.
Luisi
P L
PL
D000818:Animals; D002458:Cell Fractionation / Q000379:methods; D002942:Circular Dichroism; D004143:Dioctyl Sulfosuccinic Acid; D008099:Liver / Q000648:ultrastructure*; D008823:Micelles; D012270:Ribosomes / Q000648:ultrastructure*; D013056:Spectrophotometry, Ultraviolet; D013501:Surface-Active Agents; D013552:Swine
D008823:Micelles; D013501:Surface-Active Agents; D004143:Dioctyl Sulfosuccinic Acid