Effects of antioxidants on induction of micronuclei in rat peripheral blood reticulocytes by potassium bromate.
Micronucleus induction in male F344 rat peripheral blood by potassium bromate (KBrO3), a rat renal carcinogen, and its inhibition by several antioxidants were studied using the acridine orange supravital staining method. The frequency of micronucleated reticulocytes (MNRETs) peaked 32 h after a single i.p. treatment of rats with KBrO3 at a dose of 60 mg/kg. Co-treatment with glutathione (GSH) or cysteine (Cys) i.p. at doses of 800 mg/kg and 400 mg/kg, respectively, 30 min before and 30 min after the KBrO3 treatment significantly inhibited the micronucleus induction by KBrO3. Daily i.g. administration of vitamin C for 5 days at a dose of 200 mg/kg/day was also effective in protecting against micronucleus induction by KBrO3 given on the 4th day. However, co-treatment with superoxide dismutase in liposome-encapsulated form by i.p. injection at a dose of 18,000 U/kg 30 min before and 30 min after the KBrO3 application exerted no effect. The results indicate that antioxidants, especially sulfhydryl compounds, have protective potential against the clastogenicity of KBrO3, also suggesting that active oxygen species may play an important role in its clastogenicity.
Sai
K
K
Divisions of Toxicology, National Institute of Hygienic Sciences, Tokyo, Japan.
Hayashi
M
M
Takagi
A
A
Hasegawa
R
R
Sofuni
T
T
Kurokawa
Y
Y
D000165:Acridine Orange; D000818:Animals; D016587:Antimutagenic Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D001959:Bromates / Q000633:toxicity*; D002478:Cells, Cultured; D003545:Cysteine / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005503:Food Additives / Q000633:toxicity; D005978:Glutathione / Q000494:pharmacology*; D008297:Male; D048629:Micronuclei, Chromosome-Defective / Q000187:drug effects*; D015162:Micronucleus Tests; D008856:Microscopy, Fluorescence; D051381:Rats; D011916:Rats, Inbred F344; D012156:Reticulocytes / Q000187:drug effects
D016587:Antimutagenic Agents; D000975:Antioxidants; D001959:Bromates; D005503:Food Additives; C019536:potassium bromate; D000165:Acridine Orange; D005978:Glutathione; D003545:Cysteine