Enhanced release of immunoreactive CGRP and substance P from spinal dorsal horn slices occurs during carrageenan inflammation.
In vitro superfusion was used to examine the effect of inflammation on the release of immunoreactive calcitonin gene-related peptide (iCGRP) and substance P(iSP) from the dorsal horn of rats. Three hours after carrageenan, hindpaws exhibited hyperalgesia, edema, and hyperthermia. Spontaneous and capsaicin-evoked release of iCGRP and iSP were significantly increased following inflammation. The enhanced release of iCGRP and iSP in the dorsal horn may serve as a biochemical marker for the development of hyperalgesia.
Garry
M G
MG
Department of Restorative Sciences, University of Minnesota, Minneapolis 55455.
Hargreaves
K M
KM
D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000378:metabolism*; D002211:Capsaicin / Q000494:pharmacology; D002351:Carrageenan; D004487:Edema; D066298:In Vitro Techniques; D007249:Inflammation / Q000503:physiopathology*; D008297:Male; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains; D013116:Spinal Cord / Q000187:drug effects / Q000378:metabolism / Q000503:physiopathology*; D013373:Substance P / Q000378:metabolism*
D013373:Substance P; D002351:Carrageenan; D015740:Calcitonin Gene-Related Peptide; D002211:Capsaicin