Genomic organization and full-length cDNA sequence of human collagen X.
We have determined the full-length cDNA sequence of the human alpha 1(X) collagen gene by sequence analysis of a genomic clone ERG [(1991) Dev. Biol. 148, 562-572], and of cDNA fragments generated from a reverse transcribed as alpha 1(X) mRNA by PCR. We defined the promoter region, the transcription initiation site and the full-length 5'-untranslated region. We also report the exon/intron boundaries of the transcript and the complete 3'-untranslated region as well as a 3'-flanking sequence containing two additional polyadenylation signals. The promoter region is homologous to chicken and mouse type X promoters within several highly conserved regions. The genomic organization shows high homologies to chicken and mouse.
Reichenberger
E
E
Max-Planck Society, University of Erlangen-Nürnberg, Germany.
Beier
F
F
LuValle
P
P
Olsen
B R
BR
von der Mark
K
K
Bertling
W M
WM
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002645:Chickens; D003094:Collagen / Q000235:genetics*; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D015698:Genomic Library; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D016133:Polymerase Chain Reaction; D012045:Regulatory Sequences, Nucleic Acid; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
D009838:Oligodeoxyribonucleotides; D003094:Collagen; D004247:DNA