title: UV absorption complicates PCR decontamination.
abstract: UV irradiation is widely used to inactivate contaminating DNA in PCR. Highly UV-absorbent deoxyribonucleoside triphosphates in PCR mixtures reduce the efficiency of UV decontamination. Optimal decontamination may be achieved by irradiating the PCR mixture without the deoxyribonucleoside triphosphates.
authors.affiliation: Duke University Medical Center, Durham, NC 27710.
authors.forename: R
authors.initials: R
authors.lastname: Frothingham
authors.forename: R B
authors.initials: RB
authors.lastname: Blitchington
authors.forename: D H
authors.initials: DH
authors.lastname: Lee
authors.forename: R C
authors.initials: RC
authors.lastname: Greene
authors.forename: K H
authors.initials: KH
authors.lastname: Wilson
mesh_terms: D016477:Artifacts; D001483:Base Sequence; D004247:DNA / Q000528:radiation effects*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D014466:Ultraviolet Rays
chemical_list: D004247:DNA