MPM-12: a monoclonal antibody that predominantly stains mitotic cells and recognizes a protein kinase.
The monoclonal antibody MPM-12, raised by using partially purified extract of mitotic HeLa cells as the immunogen, preferentially stains the cytoplasm of mitotic cells by indirect immunofluorescence without exhibiting any species specificity. On immunoblots, MPM-12 recognizes three bands, of 155, 88, and 68 kDa, in mitotic HeLa cell extract but only the 68-kDa band in interphase cell extract. The 68-kDa band seems to be associated with chromatin while the other two are not. All three MPM-12 reactive peptides are phosphorylated, and the phosphorylation seems to be required for MPM-12 reactivity. The MPM-12 immunocomplexes exhibit autophosphorylating and histone H1 kinase activity.
Ganju
R K
RK
Department of Medical Oncology, University of Texas M. D. Anderson Cancer Center, Houston.
Penkala
J E
JE
Wright
D A
DA
Davis
F M
FM
Rao
P N
PN
D000911:Antibodies, Monoclonal / Q000276:immunology*; D002843:Chromatin / Q000737:chemistry / Q000276:immunology; D003593:Cytoplasm / Q000737:chemistry / Q000276:immunology*; D000939:Epitopes / Q000276:immunology; D005455:Fluorescent Antibody Technique; D006367:HeLa Cells; D006801:Humans; D007150:Immunohistochemistry; D008856:Microscopy, Fluorescence; D008938:Mitosis / Q000276:immunology*; D010748:Phosphopeptides / Q000276:immunology; D010766:Phosphorylation; D006656:Protamine Kinase / Q000276:immunology / Q000302:isolation & purification; D011494:Protein Kinases / Q000032:analysis / Q000276:immunology*; D013194:Staining and Labeling / Q000379:methods*
D000911:Antibodies, Monoclonal; D002843:Chromatin; D000939:Epitopes; D010748:Phosphopeptides; D011494:Protein Kinases; D006656:Protamine Kinase