title: Gas chromatographic headspace analysis of sevoflurane in blood.
abstract: We have developed a rapid, simple and precise gas chromatographic headspace analysis for sevoflurane in blood which circumvents problems associated with the high volatility and low blood/gas partition coefficient of this anesthetic drug. Blood standards are easily prepared by volumetric addition of a saturated aqueous solution of sevoflurane. Likewise, internal standardization is achieved using a saturated aqueous solution of halothane. Chromatographic conditions are similar to those commonly used for the analysis of blood ethanol. A simple method is also described for the preparation of stable and precise, aliquots of quality control materials for this assay.
authors.affiliation: Department of Pathology, School of Medicine, Texas Tech University Health Sciences Center, El Paso 79905.
authors.forename: M T
authors.initials: MT
authors.lastname: Watts
authors.forename: M
authors.initials: M
authors.lastname: Escarzaga
authors.forename: C H
authors.initials: CH
authors.lastname: Williams
mesh_terms: D000777:Anesthetics / Q000097:blood*; D002849:Chromatography, Gas; D004987:Ethers / Q000097:blood*; D006801:Humans; D008738:Methyl Ethers; D011786:Quality Control; D015203:Reproducibility of Results; D000077149:Sevoflurane
chemical_list: D000777:Anesthetics; D004987:Ethers; D008738:Methyl Ethers; D000077149:Sevoflurane