Serotonin inhibits adenylate cyclase in human platelet membranes.
Serotonin (5-HT), the non-specific 5-HT1 receptor agonist methysergide, and the 5-HT1B receptor agonist CGS 12066 dose-dependently inhibited forskolin-stimulated adenylate cyclase activity in membranes derived from human platelets. Buspirone and ipsapirone showed partial agonist activity. The effect of 5-HT to inhibit forskolin-stimulated activity was partially antagonised by spiperone, yohimbine and pindolol, with each of these compounds showing some partial agonist activity. It is concluded that human platelet membranes possess receptors for 5-HT coupled negatively to adenylate cyclase. The relationship of these receptors to other binding sites which have been shown for 5-HT on platelet membranes remains to be finally elucidated.
Newman
M E
ME
Yaacov Herzog Centre for Brain and Psychiatry Research, Ezrath Nashim-Herzog Hospital, Jerusalem, Israel.
D000067956:Adenylyl Cyclase Inhibitors; D000262:Adenylyl Cyclases / Q000097:blood; D001665:Binding Sites; D001792:Blood Platelets / Q000201:enzymology*; D005576:Colforsin / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008784:Methysergide / Q000494:pharmacology*; D011810:Quinoxalines / Q000494:pharmacology*; D011985:Receptors, Serotonin / Q000187:drug effects / Q000378:metabolism; D012701:Serotonin / Q000494:pharmacology*; D013134:Spiperone / Q000494:pharmacology; D015016:Yohimbine / Q000494:pharmacology
D000067956:Adenylyl Cyclase Inhibitors; D011810:Quinoxalines; D011985:Receptors, Serotonin; C052561:CGS 12066B; D005576:Colforsin; D015016:Yohimbine; D012701:Serotonin; D013134:Spiperone; D000262:Adenylyl Cyclases; D008784:Methysergide