title: Photoreactivation implicates cyclobutane dimers as the major promutagenic UVB lesions in yeast.
abstract: Previously we compared the mutational specificities of polychromatic UVB (285-320 nm) and UVC (254 nm) light in the SUP4-o gene of the yeast Saccharomyces cerevisiae. Striking similarities in the types and distributions of induced SUP4-o mutations were consistent with roles for cyclobutane dimers and pyrimidine(6-4)pyrimidone photoproducts in mutation induction by UVB. To assess the relative importance of cyclobutane dimers, we have now examined the effect of photoreactivation (PR), which specifically reverses these lesions, on UVB and UVC induction of SUP4-o mutations. PR reduced the frequencies of both UVB and UVC mutagenesis by approximately 75%. Collections of 138 and 158 SUP4-o mutants induced by treatment with UVB plus PR or UVC plus PR, respectively, were characterized by DNA sequencing and the results were compared to those for 208 UVB and 211 UVC-induced mutants analyzed earlier. PR decreased the frequency of UVB-induced G.C----A.T transitions by 85%, diminished the substitution frequencies at individual sites by 64% on average, and reduced the mutation frequencies at the five UVB hotspots by 87%. A more detailed examination revealed that the transition frequencies at the 3' base of 5'-TC-3' and 5'-CC-3' sequences were decreased by 90% and 72%, respectively. Finally, PR appeared to occur to the same extent on both the transcribed and non-transcribed strands of SUP4-o. Similar results were obtained for PR following UVC irradiation. Our findings indicate that cyclobutane dimers are responsible for the majority of UVB mutagenesis in yeast.
authors.affiliation: Department of Microbiology, University of Manitoba, Winnipeg, Canada.
authors.forename: J D
authors.initials: JD
authors.lastname: Armstrong
authors.forename: B A
authors.initials: BA
authors.lastname: Kunz
mesh_terms: D001483:Base Sequence; D004249:DNA Damage / Q000235:genetics*; D004271:DNA, Fungal / Q000235:genetics / Q000528:radiation effects*; D005800:Genes, Fungal / Q000235:genetics / Q000528:radiation effects; D008027:Light; D008969:Molecular Sequence Data; D016296:Mutagenesis; D011740:Pyrimidine Dimers / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000528:radiation effects; D014466:Ultraviolet Rays
chemical_list: D004271:DNA, Fungal; D011740:Pyrimidine Dimers