title: Expression of complement components of the alternative pathway by glioma cell lines.
abstract: Glioma cell lines express proteins of the complement alternative pathway, namely C3, factor B, factor H, and factor I. Secretion of these proteins was shown by a sensitive and specific ELISA. C3 and factor H were rapidly secreted by glioma cell line CB193 and reached a concentration of 140 ng/ml/10(6) cells after 72 h of culture. Factor B and factor I were secreted at a lower rate and reached concentrations of 25 and 15 ng/ml/10(6) cells, respectively. Western blot and immunoprecipitation experiments showed that secreted proteins were identical to the corresponding plasma proteins. For factor H, besides the well known 150-kDa species, an additional polypeptide of 45 kDa with factor H immunoreactivity was observed. This species corresponded to the N-terminal truncated form found in plasma. In preliminary experiments, we observed control of these syntheses by cytokines. IL-1 beta significantly increased C3 secretion, with no effect on factor H. Secretion of factor H was enhanced by IFN-gamma. These results show that a glioma cell line could be a useful tool to study complement biosynthesis by glial cells in humans.
authors.affiliation: European Institute for Peptide Research INSERM U-78, Bois-Guillaume, France.
authors.forename: P
authors.initials: P
authors.lastname: Gasque
authors.forename: N
authors.initials: N
authors.lastname: Julen
authors.forename: A M
authors.initials: AM
authors.lastname: Ischenko
authors.forename: C
authors.initials: C
authors.lastname: Picot
authors.forename: C
authors.initials: C
authors.lastname: Mauger
authors.forename: C
authors.initials: C
authors.lastname: Chauzy
authors.forename: J
authors.initials: J
authors.lastname: Ripoche
authors.forename: M
authors.initials: M
authors.lastname: Fontaine
mesh_terms: D003176:Complement C3 / Q000378:metabolism*; D003180:Complement C3b Inactivator Proteins / Q000235:genetics / Q000378:metabolism*; D011415:Complement Factor B / Q000235:genetics / Q000378:metabolism*; D017242:Complement Factor H; D017244:Complement Factor I; D016207:Cytokines / Q000494:pharmacology; D015870:Gene Expression / Q000187:drug effects; D005910:Glioma / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D012333:RNA, Messenger / Q000235:genetics; D012697:Serine Endopeptidases / Q000235:genetics / Q000378:metabolism*; D014407:Tumor Cells, Cultured
chemical_list: C493495:CFH protein, human; D003176:Complement C3; D003180:Complement C3b Inactivator Proteins; D016207:Cytokines; D012333:RNA, Messenger; D017242:Complement Factor H; D012697:Serine Endopeptidases; D017244:Complement Factor I; D011415:Complement Factor B