High-performance liquid chromatographic determination of naproxen, ibuprofen and diclofenac in plasma and synovial fluid in man.
High-performance liquid chromatographic assay procedures have been developed for naproxen, ibuprofen and diclofenac in human plasma and synovial fluid samples. A single liquid-liquid extraction procedure was used to isolate each compound from acidified biological matrix prior to the quantitative analysis. A Spherisorb ODS column (12.5 cm x 4.6 mm I.D.) was used for all the chromatography. Naproxen was eluted with a mobile phase of methanol-Sörensen's buffer at pH 7 (37:63, v/v). Ibuprofen and diclofenac were eluted using mobile phases of methanol-water at pH 3.3 (65:35, v/v and 63:37, v/v, respectively). Diphenylacetic acid was used as the internal standard for the assay of naproxen and flurbiprofen was used in the analysis of ibuprofen and diclofenac. Inter- and intra-day coefficients of variation were less than 7%. The assays were used in clinical studies of the three drugs in osteo- and rheumatoid arthritis patients.
Blagbrough
I S
IS
Department of Pharmaceutical Sciences, University of Nottingham, University Park, UK.
Daykin
M M
MM
Doherty
M
M
Pattrick
M
M
Shaw
P N
PN
D002851:Chromatography, High Pressure Liquid; D004008:Diclofenac / Q000097:blood / Q000378:metabolism*; D006801:Humans; D007052:Ibuprofen / Q000097:blood / Q000378:metabolism*; D009288:Naproxen / Q000097:blood / Q000378:metabolism*; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet; D013582:Synovial Fluid / Q000737:chemistry*
D004008:Diclofenac; D009288:Naproxen; D007052:Ibuprofen