C-type natriuretic peptide-22 differentiates between natriuretic peptide receptors in rat choroid plexus and subfornical organ.
Atrial natriuretic peptide (ANP) receptors in the rat choroid plexus and subfornical organ were characterized with C-type natriuretic peptide-22 (CNP-22) and 125I-Tyr0-CNP-22. The receptor autoradiographic method we used revealed that 125I-Tyr0-CNP-22 specifically bound to the choroid plexus, but only slightly to the subfornical organ, areas densely labeled with 125I-alpha-rat ANP (125I-rANP). CNP-22 significantly inhibited 125I-rANP binding to the choroid plexus; however, the peptide did not affect 125I-rANP binding to the subfornical organ. Thus, the characteristics of ANP receptors in the rat choroid plexus and subfornical organ are probably different.
Himeno
A
A
Department of Pharmacology 2, Nagasaki University School of Medicine, Japan.
Niwa
M
M
Nakao
K
K
Suga
S
S
Yamashita
K
K
Kataoka
Y
Y
Nakane
Y
Y
Imura
H
H
Taniyama
K
K
D000595:Amino Acid Sequence; D000818:Animals; D009320:Atrial Natriuretic Factor / Q000494:pharmacology*; D001345:Autoradiography; D002831:Choroid Plexus / Q000187:drug effects* / Q000378:metabolism; D066298:In Vitro Techniques; D007457:Iodine Radioisotopes; D008297:Male; D008969:Molecular Sequence Data; D020098:Natriuretic Peptide, C-Type; D009419:Nerve Tissue Proteins / Q000494:pharmacology*; D009928:Organ Specificity; D051381:Rats; D017208:Rats, Wistar; D017461:Receptors, Atrial Natriuretic Factor / Q000187:drug effects*; D013356:Subfornical Organ / Q000187:drug effects* / Q000378:metabolism
D007457:Iodine Radioisotopes; D009419:Nerve Tissue Proteins; D020098:Natriuretic Peptide, C-Type; D009320:Atrial Natriuretic Factor; D017461:Receptors, Atrial Natriuretic Factor