Immunological properties of ribosome-inactivating proteins and a saporin immunotoxin.
Antibodies have been raised in rabbits against plant ribosome-inactivating proteins (RIPs) and used to demonstrate cross-reactivity between RIPs from plants belonging to the same family, but little or no cross-reactivity between RIPs from taxonomically unrelated plants. When an immunotoxin consisting of saporin conjugated to bovine IgG was injected into rabbits, the animals formed antibodies against both saporin and bovine IgG.
Strocchi
P
P
Dipartimento di Farmacologia, Università di Bologna, Italy.
Barbieri
L
L
Stirpe
F
F
D000818:Animals; D000917:Antibody Formation; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D015151:Immunoblotting; D007074:Immunoglobulin G / Q000276:immunology; D000922:Immunotoxins / Q000276:immunology*; D008297:Male; D009699:N-Glycosyl Hydrolases; D010940:Plant Proteins / Q000276:immunology*; D011817:Rabbits; D054789:Ribosome Inactivating Proteins, Type 1; D012270:Ribosomes / Q000276:immunology; D000076985:Saporins
D007074:Immunoglobulin G; D000922:Immunotoxins; D010940:Plant Proteins; D054789:Ribosome Inactivating Proteins, Type 1; D009699:N-Glycosyl Hydrolases; D000076985:Saporins