Phagolysosomal pH and dissolution of cobalt oxide particles by alveolar macrophages.
We studied phagolysosomal pH in rabbit alveolar macrophages (AM) incubated with 0-15 microM chloroquine. There was a dose-related increase in pH with chloroquine concentration. Electron microscopy showed that chloroquine increased lysosomal size. In a second experiment we studied dissolution of radiolabeled cobalt oxide particles by rabbit AM, phagolysosomal pH, and lysosomal size. The cells were incubated for 2 days with 0, 2.5, and 10 microM chloroquine. Size and pH increased with chloroquine concentration. Dissolution of cobalt particles by the AM did not clearly change with pH. In a third experiment, dissolution of cobalt oxide particles in 0.1 M acetate buffer in saline with pH 4.0, 5.0, and 6.0 was studied. At the same pH, dissolution in acetate buffer was faster than in the AM, and the dissolution appeared to decrease faster with increasing pH than in the AM. A simple model for dissolution of a particle in a phagolysosome was proposed. This model predicts the types of differences in dissolution between AM and buffered saline.
Lundborg
M
M
Section of Lung Medicine, Institute of Environmental Medicine, Karolinska Institute, Stockholm, Sweden.
Falk
R
R
Johansson
A
A
Kreyling
W
W
Camner
P
P
D000818:Animals; D002738:Chloroquine / Q000494:pharmacology; D003035:Cobalt / Q000493:pharmacokinetics*; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D016676:Macrophages, Alveolar / Q000378:metabolism*; D008854:Microscopy, Electron; D008954:Models, Biological; D010087:Oxides / Q000493:pharmacokinetics*; D010588:Phagosomes / Q000737:chemistry* / Q000648:ultrastructure; D011817:Rabbits; D012995:Solubility
D010087:Oxides; D003035:Cobalt; D002738:Chloroquine; C060728:cobalt oxide