title: Affinity chromatography of serine proteinases from the bovine intervertebral disc on BPTI-Separon HEMA 1000.
abstract: A method for the purification of serine proteinases from the bovine intervertebral disc using affinity chromatography on basic pancreatic trypsin inhibitor (BPTI) immobilized to the hydroxyalkyl methacrylate copolymer Separon HEMA 1000 E is reported. Its advantage is the possibility of obtaining serine proteinases without an artificial alteration in relative molecular mass.
authors.affiliation: Institute of Rheumatic Diseases, Prague, Czechoslovakia.
authors.forename: J
authors.initials: J
authors.lastname: Stovícková
authors.forename: V
authors.initials: V
authors.lastname: Podrazký
authors.forename: K
authors.initials: K
authors.lastname: Trnavský
mesh_terms: D000818:Animals; D007611:Aprotinin; D002417:Cattle; D002846:Chromatography, Affinity / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D007403:Intervertebral Disc / Q000737:chemistry*; D008689:Methacrylates; D012697:Serine Endopeptidases / Q000032:analysis*
chemical_list: D008689:Methacrylates; C005044:hydroxyethyl methacrylate; D007611:Aprotinin; D012697:Serine Endopeptidases