title: Electrophoresis and silver staining of rat urinary proteins including alpha 2u-globulin (rat n II).
abstract: Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and silver staining have been used to monitor the fractionation of rat urinary proteins by Amicon Centriprep C30/C10 ultrafiltration and to assess the purification of alpha 2u-globulin, a laboratory animal allergen, precipitated by exhaustive dialysis. Factors influencing the protein composition of rat urine have been identified. Dialysis procedures have been evaluated for precipitation of alpha 2u-globulin and ultrafiltration used for further purification of the allergen.
authors.affiliation: Biochemistry Research Laboratory, School of Pharmaceutical and Chemical Sciences, Sunderland Polytecnic, UK.
authors.forename: T
authors.initials: T
authors.lastname: Marshall
authors.forename: K M
authors.initials: KM
authors.lastname: Williams
authors.forename: C
authors.initials: C
authors.lastname: Bayard
authors.forename: O
authors.initials: O
authors.lastname: Vesterberg
mesh_terms: D000510:Alpha-Globulins / Q000652:urine*; D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D008297:Male; D011507:Proteinuria / Q000652:urine*; D051381:Rats; D011919:Rats, Inbred Strains; D016622:Silver Staining; D014462:Ultrafiltration
chemical_list: D000510:Alpha-Globulins; C013803:alpha 2u globulin