[Detection of cancer cells in peritoneal fluid or washing by immunocytochemical staining with monoclonal antibodies].
Three Monoclonal Antibodies (McAbs) against ovarian carcinoma and anti-CEA McAb were used for peroxidase-antiperoxidase (PAP) staining to detect cancer cells from 42 cases of either pleural fluid or ascites and peritoneal washings of cancer patients with ovarian cancer and endometrial carcinoma. The results showed that the positive rates by PAP staining of every group were higher than that by ordinary papanicolaou staining, especially in the group of epithelial ovarian carcinoma with primary surgery. There were significant differences by these two methods (P less than 0.05). It is concluded that a suitably chosen a panel of McAbs can be used in identifying differential diagnosis in ovarian cancer and the combined use of two methods will give more help to clinics.
Zhao
Y
Y
Gynecologic Oncology Research Laboratory, People's Hospital, Beijing Medical University.
D000911:Antibodies, Monoclonal; D001202:Ascitic Fluid / Q000473:pathology*; D002272:Carcinoembryonic Antigen / Q000276:immunology; D002277:Carcinoma / Q000175:diagnosis* / Q000473:pathology; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D010051:Ovarian Neoplasms / Q000175:diagnosis* / Q000473:pathology; D013194:Staining and Labeling; D014407:Tumor Cells, Cultured; D014594:Uterine Neoplasms / Q000175:diagnosis* / Q000473:pathology
D000911:Antibodies, Monoclonal; D002272:Carcinoembryonic Antigen