Production and characterization of two new mouse monoclonal antibodies reactive with denatured mouse class II beta chains.
We report on the production and characterization of two murine peptide specific anti-major histocompatibility complex class II chain specific monoclonal antibodies. Two new mouse monoclonal antibodies reactive with two synthetic peptides corresponding to Abb amino acids (146-157) and Abs amino acids (146-157) were produced. KL295 is the mouse anti-Abb monoclonal antibody, which reacts with denatured beta chains of H-2b, H-2d, H-2p, and H-2q, but fail to react with spleen cell lysates from H-2f, H-2j, H-2k and H-2s mice. The mouse anti Abs monoclonal antibody, KL304 in contrast reacts with the denatured Class II beta chains of H-2f, H-2j, H-2k and H-2s mice, but fails to bind H-2b, H-2d, H-2p or H-2q beta chain, suggesting residues 153-155 of this molecule to be critical for the epitope. Both KL295 and KL304 bind B10.WB (H-2j) which suggests a unique epitope in this strain of mice. Neither KL295 or KL304 react with native mouse class II cell surface molecules.
LaPan
K E
KE
Department of Microbiology and Immunology, University of North Carolina, Chapel Hill 27599-7290.
Klapper
D G
DG
Frelinger
J A
JA
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D000939:Epitopes / Q000737:chemistry; D006183:H-2 Antigens / Q000737:chemistry; D000949:Histocompatibility Antigens Class II / Q000737:chemistry; D006825:Hybridomas / Q000276:immunology; D051379:Mice; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry / Q000276:immunology; D011487:Protein Conformation; D011489:Protein Denaturation
D000911:Antibodies, Monoclonal; D000939:Epitopes; D006183:H-2 Antigens; D000949:Histocompatibility Antigens Class II; D010455:Peptides