The calcium-binding proteins MRP8 and MRP14 form a membrane-associated heterodimer in a subset of monocytes/macrophages present in acute but absent in chronic inflammatory lesions.
Monocytes/macrophages expressing an epitope recognized by a monoclonal antibody 27E10 are present in acute but are absent in chronic inflammatory disorders. This report shows that the 27E10 antigen is formed by noncovalent association of the two Ca(2+)-binding proteins MRP8 and MRP14 which belong to the S100 protein family. Identification has been confirmed immunochemically, by matrix-assisted UV-laser desorption/ionization spectrometry and by partial amino acid sequencing. Surface expression of the MRP8/MRP14 complex on a subset of monocytes is reported for the first time and shown to be up-regulated in a Ca(2+)-dependent manner. The 27E10 surface-positive monocytes isolated by cell separation techniques release high amounts of tumor necrosis factor-alpha and interleukin-1 beta in contrast to their 27E10 surface-negative counterparts thus emphasizing their role in inflammation.
Bhardwaj
R S
RS
Institute of Experimental Dermatology, University of Münster, FRG.
Zotz
C
C
Zwadlo-Klarwasser
G
G
Roth
J
J
Goebeler
M
M
Mahnke
K
K
Falk
M
M
Meinardus-Hager
G
G
Sorg
C
C
D000208:Acute Disease; D000911:Antibodies, Monoclonal / Q000276:immunology; D002135:Calcium-Binding Proteins / Q000032:analysis*; D040501:Calgranulin A; D040502:Calgranulin B; D002908:Chronic Disease; D000939:Epitopes / Q000032:analysis; D006801:Humans; D007249:Inflammation / Q000378:metabolism*; D008264:Macrophages / Q000737:chemistry*; D009000:Monocytes / Q000737:chemistry*; D010641:Phenotype
D000911:Antibodies, Monoclonal; D002135:Calcium-Binding Proteins; D040501:Calgranulin A; D040502:Calgranulin B; D000939:Epitopes