Effects of ethanol and its fluorinated analogues on the calcium ATPase of sarcoplasmic reticulum.
The effects of ethanol, monofluoro- and trifluoroethanol on the hydrolysis of ATP and coupled Ca2+ translocation by sarcoplasmic reticulum Ca(2+)-ATPase (EC 3.6.1.38) were measured. All three alcohols had parallel effects on the enzyme activities at concentrations up to 200 mM, suggesting a similar mechanism of action. 19F nuclear magnetic resonance spectroscopy was used to investigate their site of action in the purified enzyme.
Bennett
F C
FC
Department of Biochemistry, University of Queensland, NSW, Australia.
Gates
S D
SD
King
G F
GF
Shanley
B C
BC
Smith
R
R
D000818:Animals; D001665:Binding Sites; D001693:Biological Transport, Active / Q000187:drug effects; D002118:Calcium / Q000378:metabolism; D000252:Calcium-Transporting ATPases / Q000037:antagonists & inhibitors*; D000431:Ethanol / Q000031:analogs & derivatives / Q000494:pharmacology*; D066298:In Vitro Techniques; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D011817:Rabbits; D012519:Sarcoplasmic Reticulum / Q000187:drug effects* / Q000378:metabolism; D014270:Trifluoroethanol / Q000494:pharmacology
D000431:Ethanol; C001236:2-fluoroethanol; D014270:Trifluoroethanol; D000252:Calcium-Transporting ATPases; D002118:Calcium