Chromosomal sublocalization of the 2;13 translocation breakpoint in alveolar rhabdomyosarcoma.
A characteristic balanced reciprocal chromosomal translocation [t(2;13)(q35;q14)] has been identified in more than 50% of alveolar rhabdomyosarcomas. As the first step in characterization of the genes involved in this translocation, we constructed somatic cell hybrids that retained either the derivative chromosome 2 or the derivative chromosome 13 without a normal chromosome 13 homologue. Ten linked DNA probes known to be located within bands 13q13-q14 were mapped relative to the breakpoint on chromosome 13, allowing localization of the breakpoint region between two loci separated by 5.5 cM. A long-range restriction map extending approximately 2,300 kb around these loci failed to provide evidence of rearrangement. Additionally, we confirmed that the FMS-like tyrosine kinase gene (FLT), previously localized to 13q12 by in situ hybridization, is located proximal to the breakpoint, and we demonstrated that FLT is not a target for disruption by this tumor-specific translocation.
Shapiro
D N
DN
Department of Hematology-Oncology, St. Jude Children's Research Hospital, Memphis, TN 38105.
Valentine
M B
MB
Sublett
J E
JE
Sinclair
A E
AE
Tereba
A M
AM
Scheffer
H
H
Buys
C H
CH
Look
A T
AT
D015139:Blotting, Southern; D002460:Cell Line; D002882:Chromosomes, Human, Pair 13; D002889:Chromosomes, Human, Pair 2; D004247:DNA / Q000032:analysis; D015342:DNA Probes; D004587:Electrophoresis, Agar Gel; D008040:Genetic Linkage; D006801:Humans; D008339:Mandibular Neoplasms / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D011518:Proto-Oncogene Proteins / Q000235:genetics; D011519:Proto-Oncogenes; D015183:Restriction Mapping; D012208:Rhabdomyosarcoma / Q000235:genetics*; D014178:Translocation, Genetic; D040281:Vascular Endothelial Growth Factor Receptor-1
D015342:DNA Probes; D011518:Proto-Oncogene Proteins; D004247:DNA; D040281:Vascular Endothelial Growth Factor Receptor-1