title: Comparison of four different enzyme-linked immunosorbent assays for serological diagnosis of Salmonella enteritidis infections in experimentally infected chickens.
abstract: The program for the eradication of Salmonella enteritidis from chickens in The Netherlands is based on bacteriological examination of breeding flocks. There is a great need for a specific and sensitive serological screening test. For that purpose, we developed four different enzyme-linked immunosorbent assays (ELISAs), i.e., an indirect ELISA with S. enteritidis flagellin, an indirect ELISA with S. enteritidis lipopolysaccharide, a double-antibody sandwich blocking ELISA that uses monoclonal antibodies against S. enteritidis flagellin (GM-DAS blocking ELISA), and a double-antibody sandwich ELISA that uses monoclonal antibodies against S. enteritidis lipopolysaccharide. In the present study, we compare the results of those ELISAs with sera from experimentally infected 1-day-old chickens and with sera and eggs from experimentally infected laying hens. Experimental infections were induced with strains of S. enteritidis phage types 1 and 2, S. typhimurium, and S. panama. Sera were collected up to days 44 and 39 after infection from 1-day-old chickens and laying hens, respectively. Only the GM-DAS blocking ELISA was able to discriminate between S. enteritidis infections and infections with the other serotypes. This ELISA had both a sensitivity and a specificity of 100% for all serum samples from experimentally infected chickens. A field study is in progress to evaluate whether this test can be implemented in the Dutch S. enteritidis eradication program.
authors.affiliation: Department of Bacteriology, DLO-Central Veterinary Institute, Lelystad, The Netherlands.
authors.forename: F G
authors.initials: FG
authors.lastname: van Zijderveld
authors.forename: A M
authors.initials: AM
authors.lastname: van Zijderveld-van Bemmel
authors.forename: J
authors.initials: J
authors.lastname: Anakotta
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D000911:Antibodies, Monoclonal; D002645:Chickens / Q000382:microbiology*; D004530:Egg Yolk / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D005408:Flagellin / Q000276:immunology; D008070:Lipopolysaccharides / Q000276:immunology; D011201:Poultry Diseases / Q000175:diagnosis*; D012481:Salmonella Infections, Animal / Q000175:diagnosis*; D012477:Salmonella enteritidis / Q000276:immunology / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000379:methods
chemical_list: D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D008070:Lipopolysaccharides; D005408:Flagellin