[Comparison of sensitivity of Hep-2 cells with that of HL cells against Chlamydia pneumoniae].
We compared the growth of Chlamydia pneumoniae, reference strain TW183 and an isolate from a Japanese infant, AC43 in HeLa229, HL and Hep-2 cells. The mean number of inclusion-forming units was significantly higher on HL cells than HeLa229 cells, when the cells were not pretreated with DEAE-dextran. When the cells were not pretreated with DEAE-dextran, Hep-2 cells had a higher mean number of inclusion-forming units and a higher yield than other cell lines. Iscove's modified Dulbecco medium enhanced growth of strain TW183 in HeLa229 cells but had a low yield of strain AC43 in Hep-2 cells.
Yoshizawa
H
H
Department of Pathobiology, School of Nursing, Chiba University.
Dairiki
K
K
Yamazaki
T
T
D001431:Bacteriological Techniques; D002460:Cell Line; D016993:Chlamydophila pneumoniae / Q000254:growth & development*; D003470:Culture Media; D006367:HeLa Cells; D006801:Humans; D008168:Lung / Q000166:cytology
D003470:Culture Media