Ionic selectivity of volume-sensitive currents in human epithelial cells.
The ion selectivity of swelling-activated Cl- currents has been investigated in three different human epithelial cell lines, two derived from the airway epithelium (9HTEo- and CFNPE9o-) and one from a colon carcinoma (T84). The relative permeability of volume-sensitive currents with respect to Cl- is: I- (1.19) greater than NO3- (1.07) approximately Br-(1.05) greater than Cl-(1.0) greater than F-(0.5) approximately HCO3-(0.48) greater than isethionate(0.28) greater than aspartate (0.14) approximately gluconate(0.13) approximately SO4(2-)(0.12). This type of ion selectivity is similar to that described for depolarization-activated outwardly rectifying Cl- channels found in epithelial cells.
Rasola
A
A
Laboratorio di Genetica Molecolare, Istituto Giannina Gaslini, Genova, Italia.
Galietta
L J
LJ
Gruenert
D C
DC
Romeo
G
G
D000838:Anions / Q000378:metabolism*; D002460:Cell Line; D018118:Chloride Channels; D002712:Chlorides / Q000378:metabolism*; D004553:Electric Conductivity; D004847:Epithelial Cells; D004848:Epithelium / Q000378:metabolism; D006801:Humans; D007038:Hypotonic Solutions / Q000494:pharmacology; D007473:Ion Channels / Q000378:metabolism*; D008564:Membrane Potentials; D008565:Membrane Proteins / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D000838:Anions; D018118:Chloride Channels; D002712:Chlorides; D007038:Hypotonic Solutions; D007473:Ion Channels; D008565:Membrane Proteins