Electrophoretic profiles of mitochondrial plasmids in Neurospora suggest they replicate by a rolling circle mechanism.
Migratory behaviour of mitochondrial plasmids from Neurospora crassa Mauriceville-1c and N. intermediate LaBelle has been studied by pulsed field gel electrophoresis (PFGE). Electrophoretic profiles demonstrate that long, linear molecules of a heterogeneous size are the prevailing form of plasmid DNA in vivo. Circular forms represent less than 8-9% of plasmid DNA. Single stranded DNA regions are abundant and lead to electrophoretic inertia of a significant amount of plasmid DNA. These profiles indicate that both plasmids replicate by the recombination dependent rolling circle mechanism.
Maleszka
R
R
Molecular and Population Genetics Group, Research School of Biological Sciences, Australian National University, Canberra.
D004261:DNA Replication; D004271:DNA, Fungal / Q000235:genetics* / Q000302:isolation & purification; D004272:DNA, Mitochondrial / Q000235:genetics* / Q000302:isolation & purification; D004277:DNA, Single-Stranded / Q000235:genetics* / Q000302:isolation & purification; D004586:Electrophoresis / Q000379:methods; D008928:Mitochondria / Q000378:metabolism*; D008970:Molecular Weight; D009492:Neurospora crassa / Q000235:genetics*; D010957:Plasmids
D004271:DNA, Fungal; D004272:DNA, Mitochondrial; D004277:DNA, Single-Stranded