Cloning, sequencing and in vivo expression of genes encoding the F0 part of the sodium-ion-dependent ATP synthase of Propionigenium modestum in Escherichia coli.
A DNA fragment containing the genes encoding subunits of the F0 part of the sodium-translocating ATPase of Propionigenium modestum was cloned in Escherichia coli and sequenced. The predicted amino acid sequences of subunits a, b and c of the P. modestum ATPase were compared with those of the corresponding subunits of proton-translocating ATPases from other bacteria and chloroplasts. Deletion mutants of E. coli, lacking different genes for ATPase subunits, were transformed with a recombinant plasmid, containing the genes for the subunits a, c, b, delta and part of alpha of the ATPase of P. modestum. Functionally reconstituted ATPase activity could be demonstrated for the transformants. The identity of the vector containing P. modestum genes was verified by restriction analysis of plasmid DNA.
Kaim
G
G
Lehrstuhl für Mikrobiologie, Technische Universität München, Federal Republic of Germany.
Ludwig
W
W
Dimroth
P
P
Schleifer
K H
KH
D000255:Adenosine Triphosphate / Q000378:metabolism; D000595:Amino Acid Sequence; D001419:Bacteria / Q000201:enzymology* / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D003001:Cloning, Molecular; D004926:Escherichia coli; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D009876:Operon; D006180:Proton-Translocating ATPases / Q000235:genetics*; D015183:Restriction Mapping; D016415:Sequence Alignment; D012964:Sodium / Q000494:pharmacology; D013045:Species Specificity
D001426:Bacterial Proteins; D046911:Macromolecular Substances; D009838:Oligodeoxyribonucleotides; D000255:Adenosine Triphosphate; D012964:Sodium; D006180:Proton-Translocating ATPases