Expression cloning of a cDNA from rabbit kidney cortex that induces a single transport system for cystine and dibasic and neutral amino acids.
We have isolated a cDNA clone by screening a rabbit kidney cortex cDNA library for expression of sodium-independent transport of L-arginine and L-alanine in Xenopus laevis oocytes. Expressed uptake relates to a single component of sodium-independent transport for dibasic and neutral amino acids. This transport activity resembles the functionally defined system b0,+ and carries cystine and dibasic amino acids with high affinity. The rBAT (b0,+ amino acid transporter-related) mRNA is found mainly in kidney and intestinal mucosa. It encodes a predicted 77.8-kDa protein with only one putative transmembrane domain and seven potential N-glycosylation sites. This protein could either be a constitutive element or a specific activator of system b0,+.
Bertran
J
J
Institute of Physiology, University of Zürich, Switzerland.
Werner
A
A
Moore
M L
ML
Stange
G
G
Markovich
D
D
Biber
J
J
Testar
X
X
Zorzano
A
A
Palacin
M
M
Murer
H
H
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000378:metabolism*; D000599:Amino Acids, Diamino / Q000378:metabolism*; D000621:Aminoisobutyric Acids / Q000378:metabolism; D000818:Animals; D001483:Base Sequence; D001692:Biological Transport; D015152:Blotting, Northern; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D003553:Cystine / Q000378:metabolism*; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D007672:Kidney Cortex / Q000378:metabolism / Q000502:physiology*; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D009865:Oocytes / Q000378:metabolism / Q000502:physiology; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D011817:Rabbits; D051381:Rats; D014982:Xenopus laevis
D000596:Amino Acids; D000599:Amino Acids, Diamino; D000621:Aminoisobutyric Acids; D002352:Carrier Proteins; D008565:Membrane Proteins; C100049:2-aminoisobutyric acid; D003553:Cystine; D012313:RNA; D004247:DNA