Slow axonal transport of soluble proteins and calpain in retinal ganglion cells of aged rabbits.
The rate of slow axonal transport of soluble proteins in retinal ganglion cells of the rabbit decreased with approximately 25% in aged (6 years) compared to previous estimates in adult (2 years) animals. Immunobinding of calpain to microtiter plates coated with a monoclonal antibody to mu-calpain was used to isolate labelled axonally transported mu-calpain from the nerve extracts. It was found that the distribution of labelled mu-calpain in the retrobulbar optic pathway was similar to the distribution profile of the slowly migrating phase of soluble proteins.
Karlsson
J O
JO
Department of Biochemistry, Tokyo Metropolitan Institute of Gerontology, Japan.
Inomata
M
M
Kawashima
S
S
D000375:Aging / Q000502:physiology; D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D000911:Antibodies, Monoclonal; D001370:Axonal Transport / Q000502:physiology*; D015153:Blotting, Western; D002154:Calpain / Q000378:metabolism*; D005136:Eye Proteins / Q000378:metabolism*; D009900:Optic Nerve / Q000378:metabolism; D011817:Rabbits; D012165:Retinal Ganglion Cells / Q000378:metabolism*; D013462:Sulfur Radioisotopes; D014795:Visual Pathways / Q000166:cytology / Q000378:metabolism
D000596:Amino Acids; D000911:Antibodies, Monoclonal; D005136:Eye Proteins; D013462:Sulfur Radioisotopes; D002154:Calpain