title: Evidence that most of the radioimmunoassayable inhibin secreted by the corpus luteum of the common marmoset monkey is of a non-dimeric form.
abstract: Although recent data for several species of primate, including human and marmoset, indicate that the corpus luteum secretes high levels of radioimmunoassayable inhibin, the nature of the immunoreactive (ir) inhibin detected has not been established. In this study, plasma ir-inhibin levels during the ovarian cycle of the marmoset (n = 12 animals) were measured by alpha-subunit-directed inhibin RIA, and values were compared with those estimated by a recently developed two-site immunoradiometric assay (IRMA) specific for inhibin alpha-beta dimer. Consistent with earlier data, plasma levels of ir-inhibin measured by RIA (overall mean value 133 +/- 7 ng/ml; n = 171) reached values 4-fold higher (p less than 0.001) during the luteal phase (222 +/- 20 ng/ml) than during the follicular phase (58 +/- 8 ng/ml), being directly correlated with plasma progesterone levels (r = 0.65; p less than 0.001). In contrast, plasma ir-inhibin levels estimated by IRMA were substantially lower than those measured by RIA (overall mean value 9.62 +/- 1.08 ng/ml; n = 171) and did not vary significantly during the cycle. Administration of a luteolytic dose of cloprostenol during the late luteal phase/early pregnancy led to an abrupt fall in plasma concentrations of progesterone (95%) and alpha-inhibin measured by RIA (82%), whereas dimeric inhibin levels remained unchanged. Analysis of marmoset luteal extracts (n = 5) by RIA, IRMA, and inhibin bioassay yielded inhibin estimates of 102.6 +/- 21.0, 0.632 +/- 0.103, and less than 2.0 ng/mg, respectively, thus confirming that only a very small proportion of the inhibin produced was dimeric (i.e., bioactive) in nature.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Biochemistry & Physiology, University of Reading, Whiteknights, United Kingdom.
authors.forename: P G
authors.initials: PG
authors.lastname: Knight
authors.forename: S
authors.initials: S
authors.lastname: Muttukrishna
authors.forename: N
authors.initials: N
authors.lastname: Groome
authors.forename: G E
authors.initials: GE
authors.lastname: Webley
mesh_terms: D000818:Animals; D002144:Callithrix / Q000502:physiology*; D003008:Cloprostenol / Q000494:pharmacology; D003338:Corpus Luteum / Q000378:metabolism*; D005260:Female; D015592:Immunoradiometric Assay; D007265:Inhibins / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D008183:Luteal Phase / Q000187:drug effects / Q000502:physiology; D008597:Menstrual Cycle / Q000187:drug effects / Q000502:physiology; D011374:Progesterone / Q000097:blood; D011487:Protein Conformation; D011863:Radioimmunoassay
chemical_list: D003008:Cloprostenol; D011374:Progesterone; D007265:Inhibins