CD59 molecule: a second ligand for CD2 in T cell adhesion.
The T cell surface molecule CD2 forms, with its counter-receptor CD58 (LFA-3), a powerful adhesion/activation pathway. There is some evidence that CD2 might bind more than a single ligand. Chinese hamster ovary cells (CHO) expressing human CD59 after cDNA transfection (CD59+CHO) form rosettes with human T cells; these rosettes are inhibited in a dose-dependent fashion by the CD59 monoclonal antibody (mAb) H19 and by the CD2 mAb O275 known to block natural rosettes, but not by the CD2R mAb D66, a poor rosette blocker. CD2+CHO transfectants form rosettes with human erythrocytes; these rosettes are inhibited by the CD59 mAb H19 in addition to CD2 mAb O275 and CD58 mAb; murine thymoma cells expressing human CD2 form rosettes with CD59+CHO cells that again are blocked by CD59 H19 and by CD2 O275 mAb. In a marked contrast with H19, two others CD59 mAb, YTH 53.1 and MEM 43, which react with a different epitope on CD59, led to a 50%-70% increase of the number of cells forming rosettes. In addition to rosette experiments, the binding of 125I-labeled CD59 molecules to CD2+CHO cells was specifically inhibited by unlabeled CD59 molecule and CD2 mAb. Furthermore, the binding of CD59 molecules to resting T cells induced expression of CD2R epitopes. These results directly show that CD2 binds CD59 and that subtle molecular changes occur upon binding.
Deckert
M
M
INSERM U343, Faculté de Médecine, Nice, France.
Kubar
J
J
Zoccola
D
D
Bernard-Pomier
G
G
Angelisova
P
P
Horejsi
V
V
Bernard
A
A
D000818:Animals; D015703:Antigens, CD / Q000032:analysis / Q000378:metabolism*; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis / Q000378:metabolism*; D018801:CD2 Antigens; D018957:CD59 Antigens; D016466:CHO Cells; D002448:Cell Adhesion; D002478:Cells, Cultured; D006224:Cricetinae; D000939:Epitopes; D006801:Humans; D008024:Ligands; D008562:Membrane Glycoproteins / Q000032:analysis / Q000378:metabolism*; D011971:Receptors, Immunologic / Q000032:analysis / Q000378:metabolism*; D013601:T-Lymphocytes / Q000502:physiology*; D014162:Transfection
D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D018801:CD2 Antigens; D018957:CD59 Antigens; D000939:Epitopes; D008024:Ligands; D008562:Membrane Glycoproteins; D011971:Receptors, Immunologic