Use of in vitro-transcribed RNAs as immobilized targets during RNA:RNA hybridization.
We describe the use of in vitro-transcribed complementary RNAs as filter-bound targets during nuclear run-on analyses. Use of these single-stranded reagents in high-stringency RNA:RNA hybridizations increases signal-to-background hybridization seen using DNA targets and allows efficient measurement of transcriptional rates across genes in either direction.
Flaspohler
J A
JA
Department of Molecular Genetics and Biochemistry, Univ. of Pittsburgh Sch. of Med., PA 15261-2072.
Milcarek
C
C
D000818:Animals; D005821:Genetic Techniques; D008566:Membranes; D051379:Mice; D009693:Nucleic Acid Hybridization; D012313:RNA / Q000032:analysis* / Q000235:genetics; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured
D012313:RNA