title: Inoculation of conventionally and specific-pathogen-free reared rabbits with Ostertagia ostertagi isolated from cattle.
abstract: Eleven trials were conducted to collect helminthologic and pathologic data from 27 conventionally reared (CR) and 53 specific-pathogen-free (SPF) 6- to 13-week-old male New Zealand White rabbits. These rabbits were given 50,000 to 100,000 ensheathed third-stage infective Ostertagia ostertagi larvae (L3) orally. The L3 had been isolated from the feces of cattle. Fecal egg counts were conducted and worm populations were determined after euthanasia 3 to 56 days after inoculation. At necropsy, nodules were observed in a confluent pattern in the mucosa of cardiac region of the stomach in all inoculated rabbits, except in CR rabbits inoculated at 6 weeks of age, which had no nodules or worm burdens 42 days after inoculation. Confluent areas were not observed in SPF rabbits euthanatized 5 days or less after inoculation; however, small, transparent nodules were evident in the mucosa of the cardiac region of the stomach. Fourth-stage larvae (L4) were obtained from the mucosal nodules after digestion of stomach of both types of rabbits. There were more L4 in SPF than CR rabbits. In addition, greater numbers of L4 were found in SPF rabbits euthanatized 14 days or less after inoculation. Petechial hemorrhages were in the fundic area of the stomach mucosa in SPF rabbits inoculated with 100,000 L3 and euthanatized 14 days later. Mature O ostertagi or worm eggs in feces were not found in inoculated CR or SPF rabbits. The pathologic changes had characteristics similar to those in cattle and goats infected with O ostertagi.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Lilly Research Laboratories, Division of Eli Lilly & Co, Greenfield, IN 46140.
authors.forename: R J
authors.initials: RJ
authors.lastname: Boisvenue
authors.forename: M N
authors.initials: MN
authors.lastname: Novilla
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000469:parasitology / Q000473:pathology*; D004195:Disease Models, Animal; D005753:Gastric Mucosa / Q000469:parasitology / Q000473:pathology; D007814:Larva / Q000254:growth & development / Q000302:isolation & purification; D008297:Male; D010028:Ostertagia / Q000254:growth & development / Q000302:isolation & purification; D010029:Ostertagiasis / Q000469:parasitology / Q000473:pathology / Q000662:veterinary*; D011817:Rabbits; D013047:Specific Pathogen-Free Organisms; D013270:Stomach / Q000469:parasitology / Q000473:pathology