Homologous sucrose synthase genes in barley (Hordeum vulgare) are located in chromosomes 7H (syn. 1) and 2H. Evidence for a gene translocation?
The chromosomal location of the two types of sucrose synthase genes, Ss1 and Ss2, has been investigated in barley by Southern blot analysis of wheat-barley addition lines using non-cross-hybridizing-specific probes corresponding to the C-terminal regions of their respective cDNA clones (congruent to 250 bp). The Ss1 gene, whose cDNA of 2,667 bp has been entirely sequenced, is located in the beta-arm of chromosome 7H (syn. 1), while that corresponding to the homologous Ss2 is in the short arm of 2H, suggesting the existence of a translocation event between these two chromosomes in cultivated barley after an initial gene duplication and divergent evolution.
Sánchez de la Hoz
P
P
Cátedra de Bioquímica y Biología Molecular, ETS Ingenieros Agrónomos-UPM, Madrid, Spain.
Vicente-Carbajosa
J
J
Mena
M
M
Carbonero
P
P
D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004247:DNA; D005964:Glucosyltransferases / Q000235:genetics*; D001467:Hordeum / Q000201:enzymology* / Q000235:genetics; D008969:Molecular Sequence Data; D012689:Sequence Homology, Nucleic Acid; D014178:Translocation, Genetic
D004247:DNA; D005964:Glucosyltransferases; C022699:sucrose synthase