Interleukin-1 beta induces the expression of an isoform of nitric oxide synthase in insulin-producing cells, which is similar to that observed in activated macrophages.
The suppressive and cytotoxic effects of interleukin-1 beta (IL-1 beta) on rodent insulin-producing cells observed in vitro are probably mediated through formation of nitric oxide (NO). In this study we demonstrate that IL-1-induced NO formation in isolated rat islets and insulin-producing HIT cells is more sensitive to inhibition by NG-monomethyl-L-arginine than to inhibition by NG-nitro-L-arginine, thus suggesting that IL-1-exposed insulin-producing cells express an isoform of nitric oxide synthase similar to that present in activated macrophages. Furthermore, IL-1 beta markedly increased the mRNA levels of the inducible macrophage form of nitric oxide synthase in HIT cells.
Eizirik
D L
DL
Department of Medical Cell Biology, Uppsala University, Sweden.
Cagliero
E
E
Björklund
A
A
Welsh
N
N
D000594:Amino Acid Oxidoreductases / Q000096:biosynthesis*; D000818:Animals; D002460:Cell Line; D004790:Enzyme Induction; D006801:Humans; D007375:Interleukin-1 / Q000031:analogs & derivatives / Q000494:pharmacology*; D007515:Islets of Langerhans / Q000187:drug effects / Q000201:enzymology*; D007527:Isoenzymes / Q000096:biosynthesis*; D008262:Macrophage Activation; D008264:Macrophages / Q000201:enzymology* / Q000276:immunology; D008297:Male; D019001:Nitric Oxide Synthase; D009573:Nitrites / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats
D007375:Interleukin-1; D007527:Isoenzymes; D009573:Nitrites; D012333:RNA, Messenger; D019001:Nitric Oxide Synthase; D000594:Amino Acid Oxidoreductases