Inhibition of gastric mucosal laminin receptor by Helicobacter pylori lipopolysaccharide: effect of ebrotidine.
The effect of lipopolysaccharide from H. pylori, a bacteria implicated in the etiology of gastric disease, on the gastric mucosal laminin-receptor interaction was investigated. The receptor protein, prepared from rat gastric epithelial cell membrane by affinity chromatography on laminin-coupled Sepharose, was radioiodinated, and incorporated into liposomes which exhibited specific affinity towards laminin-coated surface. The binding was inhibited by H. pylori lipopolysaccharide, which caused a maximum inhibition of 96% at 50 micrograms/ml. The inhibitory effect of the lipopolysaccharide was prevented by an antiulcer agent, ebrotidine that evoked essentially complete restoration in binding at 6-8 micrograms/ml. The results demonstrate that H. pylori through its lipopolysaccharide interferes with gastric epithelial cell-laminin binding, and that this disruptive effect could be successfully countered by ebrotidine.
Piotrowski
J
J
Research Center, New Jersey Dental School, University of Medicine and Dentistry of New Jersey, Newark 07103-2400.
Morita
M
M
Slomiany
A
A
Slomiany
B L
BL
D000818:Animals; D001557:Benzenesulfonates / Q000494:pharmacology*; D005753:Gastric Mucosa / Q000187:drug effects / Q000378:metabolism*; D016480:Helicobacter pylori / Q000378:metabolism*; D066298:In Vitro Techniques; D007797:Laminin / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000378:metabolism*; D051381:Rats; D011946:Receptors, Antigen / Q000037:antagonists & inhibitors / Q000187:drug effects / Q000378:metabolism; D011971:Receptors, Immunologic / Q000037:antagonists & inhibitors* / Q000187:drug effects / Q000378:metabolism; D017474:Receptors, Laminin; D013844:Thiazoles / Q000494:pharmacology*
D001557:Benzenesulfonates; D007797:Laminin; D008070:Lipopolysaccharides; D011946:Receptors, Antigen; D011971:Receptors, Immunologic; D017474:Receptors, Laminin; D013844:Thiazoles; C075156:ebrotidine