title: Absence of genotoxicity of potato alkaloids alpha-chaconine, alpha-solanine and solanidine in the Ames Salmonella and adult and foetal erythrocyte micronucleus assays.
abstract: To assess whether reported toxicities of potato-derived glycoalkaloids could be the result of interactions with cellular DNA, the genotoxic effects of alpha-solanine, alpha-chaconine and solanidine were studied, using the Ames test (Salmonella strains TA98 and TA100), the mouse peripheral blood micronucleus test and the mouse transplacental micronucleus test. The Ames test for mutagenicity with alpha-solanine was weakly positive in TA100 with S-9 activation (29 revertants per millimole per plate). However, pooled data from duplicate tests gave a negative effect. Pooled data from two experiments with alpha-chaconine gave a weak positive response in TA98 without microsomes (17 revertants per millimole per plate). The micronucleus tests for clastogenicity using male mouse and foetal blood were negative. The absence of mutagenicity and clastogenicity suggests lack of damage to intracellular DNA for potato alkaloid toxicity.
authors.affiliation: Food Safety Research Unit, USDA-ARS Western Regional Research Center, Albany, CA 94710.
authors.forename: M
authors.initials: M
authors.lastname: Friedman
authors.forename: P R
authors.initials: PR
authors.lastname: Henika
mesh_terms: D000818:Animals; D004144:Diosgenin; D005260:Female; D005333:Fetus / Q000187:drug effects; D007274:Injections, Intraperitoneal; D008297:Male; D051379:Mice; D015162:Micronucleus Tests; D009026:Mortality; D009152:Mutagenicity Tests; D009153:Mutagens / Q000633:toxicity*; D012486:Salmonella typhimurium / Q000187:drug effects; D012991:Solanaceous Alkaloids / Q000008:administration & dosage / Q000633:toxicity*; D012992:Solanine / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000633:toxicity*; D011198:Solanum tuberosum / Q000737:chemistry*
chemical_list: D009153:Mutagens; D012991:Solanaceous Alkaloids; D012992:Solanine; C011860:alpha-chaconine; D004144:Diosgenin; C013255:solanidine