Monoclonal antibody analysis of phosphatidylserine and protein kinase C localizations in developing rat cerebellum.
Understanding the topographical relationships between phosphatidylserine (PS) and protein kinase C (PKC) within neurons can provide clues about the mechanism of translocation and activation of PKC. For this purpose we applied monoclonal antibodies (Abs) of PS and PKC to sections of developing rat cerebellum. The anti-PKC Ab immunohistochemical pattern showed homogeneous staining of Purkinje cells over various postnatal ages, whereas the anti-PS Ab staining showed a heterogeneous localization over these ages. Purkinje cells did not stain well between postnatal day 14 (PND 14) and PND 21, suggesting that the PS was lost from the membrane during preparation of the sections during this period. These data imply that interactions between PS and PKC vary in Purkinje cells during postnatal development.
Miyazawa
A
A
Department of Human Basic Sciences, School of Human Sciences, Waseda University, Tokorozawa, Japan.
Inoue
H
H
Yoshioka
T
T
Horikoshi
T
T
Yanagisawa
K
K
Umeda
M
M
Inoue
K
K
D000375:Aging / Q000378:metabolism; D000818:Animals; D000911:Antibodies, Monoclonal; D002531:Cerebellum / Q000254:growth & development / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism; D007150:Immunohistochemistry; D009419:Nerve Tissue Proteins / Q000494:pharmacology; D010718:Phosphatidylserines / Q000378:metabolism*; D011493:Protein Kinase C / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014018:Tissue Distribution
D000911:Antibodies, Monoclonal; D009419:Nerve Tissue Proteins; D010718:Phosphatidylserines; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate