[Localization of the antigen in erythrocytic stages of Plasmodium yoelii by immuno-electron microscopy].
In this study, the antigen recognized by the protective McAb M26-32 in erythrocytic stages of P. yoelii was localized by immuno-electron microscopy with LR Whithe resin embedding and colloidal gold probe cytochemical techniques. The results indicated that the antigen which reacts specifically to McAb M26-32 was mainly localized within the cytoplasm of early and late trophozoites, schizonts and merozoites, being the common antigen of asexual blood stages of the plasmodium. The amount of the antigen was on the increase during the development of trophozoite, while a portion of the antigen might be transported outward by exocytosis of the parasites and then be localized in the cytoplasm of the infected erythrocytes adjacent to the parasites.
Wu
L J
LJ
Institute of Parasitic Diseases, Chinese Academy of Preventive Medicine, Shanghai.
Liu
E X
EX
Li
W L
WL
Zhu
Z Y
ZY
Pan
Y R
YR
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000953:Antigens, Protozoan / Q000032:analysis* / Q000648:ultrastructure; D004912:Erythrocytes / Q000276:immunology* / Q000469:parasitology; D051379:Mice; D016253:Microscopy, Immunoelectron; D010967:Plasmodium yoelii / Q000276:immunology* / Q000648:ultrastructure
D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan