title: Culture and initial characterization of the secretory response of neoplastic cat mast cells.
abstract: Mast cells isolated from feline splenic mastocytomas were cultured to study their structural and functional properties. Isolated cells from various cats were grown as monolayer cultures for a mean of 56 days (range, 30 to 76 days). Cat mast cells released allergic mediators in response to compound 48/80, anti-cat serum antibodies, and concanavalin A. On the basis of the finding that secretion from cat mast cells was stimulated by anti-cat serum antibodies and concanavalin A, these cells contain surface-bound immunoglobulins. The presence of mast cell-sensitizing antibodies has been suspected in cats, but never before directly demonstrated. Cultured cat mast cells have cytochemical and functional characteristics common to connective tissue-type mast cells and provide one of the few non-rodent models of cultured cells for the study of this type of mast cell.
authors.affiliation: Department of Veterinary Pathology, School of Veterinary Medicine, University of California, Davis 95616.
authors.forename: F C
authors.initials: FC
authors.lastname: Mohr
authors.forename: S K
authors.initials: SK
authors.lastname: Dunston
mesh_terms: D000818:Animals; D002371:Cat Diseases / Q000276:immunology / Q000473:pathology*; D002415:Cats; D002452:Cell Count / Q000662:veterinary; D002470:Cell Survival; D003594:Cytoplasmic Granules / Q000378:metabolism / Q000648:ultrastructure; D006636:Histamine Release; D006651:Histocytochemistry; D008407:Mast Cells / Q000276:immunology / Q000378:metabolism* / Q000648:ultrastructure; D012515:Mast-Cell Sarcoma / Q000276:immunology / Q000378:metabolism / Q000662:veterinary*; D008854:Microscopy, Electron; D013160:Splenic Neoplasms / Q000276:immunology / Q000378:metabolism / Q000662:veterinary*; D014407:Tumor Cells, Cultured; D001619:beta-N-Acetylhexosaminidases / Q000378:metabolism
chemical_list: D001619:beta-N-Acetylhexosaminidases