Histochemical and immunohistochemical study of the mucosal lymphoid system in swine.
Enzyme histochemical and immunohistochemical techniques were used to examine palatine tonsils and aggregated lymphoid follicles (Peyer's patches) of the ileum in 6- to 9-day-old and in 6-month-old pigs. Histochemical techniques were used to detect alpha-naphthyl-acetate esterase (ANAE), alpha-naphthyl-butyrate esterase (ANBE), beta-glucuronidase, adenosine triphosphatase (ATPase), and acid phosphatase (AcP). Nonspecific esterases (ANAE, ANBE) were detected in macrophages, T-cell area lymphocytes, eosinophils, fibroblastic reticular cells (FRC), follicular dendritic cells (FDC), and interdigitating cells (IDC). beta-Glucuronidase reactivity was strong in macrophages, eosinophils, FDC, and IDC, and weaker in FRC. Adenosine triphosphatase reactivity was detected in B-cell area lymphocytes, FDC, FRC, and IDC. Cell types with acid phosphatase reactivity were macrophages, FDC, FRC, and IDC. Nonepithelial cells of tonsils and aggregated lymphoid follicles of the ileum had similar enzymatic reactions. In Peyer's patches, however, epithelial cells were positive for all enzymes studied; in tonsils, only nonspecific esterases were detected. Immunoperoxidase techniques were used to detect S-100 protein and cytoplasmic immunoglobulins (IgG, IgM, and IgA). The S-100 protein was detected in lymphocytes, FDC, and FRC of tonsils and Peyer's patches; in tonsillar epithelial and endothelial cells; and in IDC of Peyer's patches.(ABSTRACT TRUNCATED AT 250 WORDS)
Ramos
J A
JA
Departamento de Patología Animal, Facultad de Veterinaria, Universidad Autónoma de Barcelona, Spain.
Ramis
A J
AJ
Marco
A
A
Domingo
M
M
Rabanal
R
R
Ferrer
L
L
D000135:Acid Phosphatase / Q000032:analysis; D000251:Adenosine Triphosphatases / Q000032:analysis; D000818:Animals; D002265:Carboxylic Ester Hydrolases / Q000032:analysis; D003713:Dendritic Cells / Q000737:chemistry / Q000201:enzymology; D004804:Eosinophils / Q000737:chemistry / Q000201:enzymology; D005260:Female; D005347:Fibroblasts / Q000737:chemistry / Q000201:enzymology; D005966:Glucuronidase / Q000032:analysis; D006651:Histocytochemistry; D007136:Immunoglobulins / Q000032:analysis; D007150:Immunohistochemistry; D008214:Lymphocytes / Q000737:chemistry / Q000201:enzymology; D008264:Macrophages / Q000737:chemistry / Q000201:enzymology; D008297:Male; D009283:Naphthol AS D Esterase / Q000032:analysis; D014066:Palatine Tonsil / Q000166:cytology* / Q000201:enzymology; D010581:Peyer's Patches / Q000166:cytology* / Q000201:enzymology; D009418:S100 Proteins / Q000032:analysis; D013047:Specific Pathogen-Free Organisms; D013552:Swine / Q000033:anatomy & histology / Q000276:immunology*
D007136:Immunoglobulins; D009418:S100 Proteins; D009283:Naphthol AS D Esterase; D002265:Carboxylic Ester Hydrolases; C021855:naphthylbutyrate esterase; D000135:Acid Phosphatase; D005966:Glucuronidase; D000251:Adenosine Triphosphatases