A ten-minute protocol for transforming Saccharomyces cerevisiae by electroporation.
We present a simplified and rapid method for the transformation of yeast cells by electroporation. Stationary cells, scraped off the agar of Petri dish cultures stored in the refrigerator for up to 6 weeks, are suspended in sorbitol buffer, spun down by gentle centrifugation, transferred into the electroporation cuvette, and immediately subjected to transformation via electroporation. Transformation efficiency of this 10-min method, which does not require the preparation of cell cultures, is about 10% of the hitherto best performing transformation procedure using cells of defined growth phase.
Grey
M
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Institut für Mikrobiologie der J. W. Goethe-Universität, Frankfurt/Main, Federal Republic of Germany.
Brendel
M
M
D004271:DNA, Fungal; D004560:Electricity; D010957:Plasmids; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D014162:Transfection / Q000379:methods*; D014170:Transformation, Genetic
D004271:DNA, Fungal