Diacylglycerol-stimulated formation of actin nucleation sites at plasma membranes.
Diacylglycerols, which are generated during phospholipase-catalyzed hydrolysis of phospholipids, stimulated actin polymerization in the presence of highly purified plasma membranes from the cellular slime mold Dictyostelium discoideum. The increased rate of actin polymerization apparently resulted from de novo formation of actin nucleation sites rather than uncapping of existing filament ends, because the membranes lacked detectable endogenous actin. The increased actin nucleation was mediated by a peripheral membrane component other than protein kinase C, the classical target of diacylglycerol action. These results indicate that diacylglycerols increase actin nucleation at plasma membranes and suggest a mechanism whereby signal transduction pathways may control cytoskeletal assembly.
Shariff
A
A
Cell Biology Group, Worcester Foundation for Experimental Biology, Shresbury, MA 01545.
Luna
E J
EJ
D000199:Actins / Q000378:metabolism*; D000470:Alkaloids / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism*; D004023:Dictyostelium / Q000378:metabolism*; D004075:Diglycerides / Q000494:pharmacology*; D007700:Kinetics; D046911:Macromolecular Substances; D009281:Naphthalenes; D011083:Polycyclic Compounds / Q000494:pharmacology; D011493:Protein Kinase C / Q000037:antagonists & inhibitors; D019311:Staurosporine; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D013997:Time Factors
D000199:Actins; D000470:Alkaloids; D004075:Diglycerides; D046911:Macromolecular Substances; D009281:Naphthalenes; D011083:Polycyclic Compounds; C044295:1,2-dioctanoylglycerol; C058807:1,3-dicapryloylglycerol; D011493:Protein Kinase C; D019311:Staurosporine; C058819:calphostin C; D013755:Tetradecanoylphorbol Acetate; D002118:Calcium