A simple and convenient method for gaining pure populations of lymphocytes at the first mitotic division in vitro.
This paper describes a simple method for obtaining pure populations of human lymphocytes at the first in vitro mitotic division (M-1) by continuous treatment with colchicine or colcemid. When colchicine (7.2 x 10(-8) M) or colcemid (4.8 x 10(-8) M) was added to cultures 0-24 h after initiation, cultures harvested at 54 h had more than 99.9% of mitotic cells in the first metaphase (M-1). We showed that not only more analyzable M-1 cells may be obtained, but staining for sister-chromatid differentiation may be avoided.
Chen
D Q
DQ
Laboratory of Industrial Hygiene, Ministry of Public Health, Deshengmenwai, Beijing, China.
Zhang
C Y
CY
D000328:Adult; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D003078:Colchicine / Q000494:pharmacology; D003703:Demecolcine / Q000494:pharmacology; D005720:Gamma Rays; D006801:Humans; D008214:Lymphocytes / Q000166:cytology* / Q000187:drug effects / Q000528:radiation effects; D008875:Middle Aged; D008938:Mitosis; D008941:Spindle Apparatus / Q000187:drug effects
D003078:Colchicine; D003703:Demecolcine