[Cultures of islet cells obtained from the fetal bovine pancreas].
The pancreas from bovine fetuses of 27-35 cm crown-rump length were used as a source of islet cell cultures. Pancreatic tissue was treated by collagenase, filtered through the metal sieve and incubated in MEM for 24 h. Using this method, cultures similar to so-called pseudo-islets were obtained. Aldehyde-fuchsin staining and electron microscopy revealed a significant number of beta-cells within the pseudo-islets, the insulin-producing activity of which was confirmed by RIA.
Kirsanova
L A
LA
Ablamunits
V G
VG
Skaletskiĭ
N N
NN
Severin
V V
VV
D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D003470:Culture Media; D005333:Fetus; D007515:Islets of Langerhans / Q000166:cytology / Q000196:embryology; D008854:Microscopy, Electron; D011863:Radioimmunoassay; D013194:Staining and Labeling
D003470:Culture Media