Nucleotide sequence of cDNA for Acacia confusa trypsin inhibitor and implication of post-translation processing.
Synthetic oligonucleotides corresponding to all possible sequences of N-terminal and C-terminal region of Acacia confusa trypsin inhibitor were used to generate ACTI-related sequences using the polymerase chain reaction on the cDNAs encoding ACTI of the seeds of legume, A. confusa. The deduced amino acid sequence agreed with that determined by the peptide analysis except an extra amino acid residue, serine, was found at the junction of A and B chain, which was removed by post-translation processing with specific protease(s). The substrate specificity of the protease(s) was found to cleave at the C-terminal sites of asparagine and serine, which was also shown to be the same case for another plant protein, abrin, isolated from legume, Abrus precatorius.
Hung
C H
CH
Institute of Biochemistry, College of Medicine, National Taiwan University, Taipei, ROC.
Lee
M C
MC
Lin
J Y
JY
D000045:Acacia; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D007887:Fabaceae / Q000235:genetics* / Q000378:metabolism; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D010940:Plant Proteins / Q000235:genetics*; D010946:Plants, Medicinal; D011061:Poly A / Q000235:genetics / Q000302:isolation & purification; D016133:Polymerase Chain Reaction / Q000379:methods; D011499:Protein Processing, Post-Translational; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012333:RNA, Messenger; D012639:Seeds / Q000502:physiology; D012689:Sequence Homology, Nucleic Acid; D014361:Trypsin Inhibitors / Q000235:genetics*
D046911:Macromolecular Substances; D009838:Oligodeoxyribonucleotides; D010940:Plant Proteins; D012333:RNA, Messenger; D014361:Trypsin Inhibitors; C074637:ACTI protein, Acacia confusa; D011061:Poly A; D012313:RNA; D004247:DNA