Detection of cell-cycle stage by fluorescence in situ hybridization: its application in human interphase cytogenetics.
Distinct cell-cycle-dependent changes in the conformation of centromeric chromatin in a specific human chromosome containing alpha-satellite DNA have been demonstrated by fluorescence in situ hybridization (FISH). This method, based upon specific FISH signal morphology, allows simultaneous analysis of chromosomal aneuploidy and detection of specific cell-cycle stage(s) of human tumor and/or normal cell populations in a single preparation of interphase cells. This interphase cytogenetic procedure might prove useful for both basic and clinical research involving human cells.
Mukherjee
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Laboratory of Cancer Genetics, Sloan-Kettering Institute, New York, NY.
Murty
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Chaganti
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D002453:Cell Cycle; D002460:Cell Line; D002503:Centromere; D002881:Chromosomes, Human, Pair 12; D003582:Cytogenetics / Q000379:methods; D006801:Humans; D017403:In Situ Hybridization; D007399:Interphase; D007621:Karyotyping; D007700:Kinetics; D008297:Male; D013050:Spectrometry, Fluorescence; D014407:Tumor Cells, Cultured