Enzyme labeling in steroid enzyme immunoassays. The p-nitrophenyl ester method for alkaline phosphatase and glucose oxidase labelings.
The p-nitrophenyl ester method was assessed as an enzyme labeling technique. The active ester of a carboxylated testosterone derivative was treated with alkaline phosphatase and glucose oxidase to give labeled antigens, using various molar ratios of steroid to enzyme. Satisfactory immunoreactivities with an anti-testosterone antibody in an enzyme immunoassay system were obtained with the labeled antigens prepared at pH 8.5 by the use of molar ratios higher than 30 and 10, respectively, in the alkaline phosphatase and glucose oxidase labelings.
Hosoda
H
H
Pharmaceutical Institute, Tohoku University, Sendai, Japan.
Fukuda
K
K
Goto
J
J
D000469:Alkaline Phosphatase; D000818:Animals; D004952:Esters / Q000032:analysis; D005949:Glucose Oxidase; D006041:Goats; D007124:Immunoenzyme Techniques; D009596:Nitrophenols; D009943:Organophosphorus Compounds; D011817:Rabbits; D013256:Steroids / Q000032:analysis*; D013739:Testosterone / Q000032:analysis
D004952:Esters; D009596:Nitrophenols; D009943:Organophosphorus Compounds; D013256:Steroids; C008644:nitrophenylphosphate; D013739:Testosterone; D005949:Glucose Oxidase; D000469:Alkaline Phosphatase