Developmental expression of voltage-sensitive K+ channels in mouse skeletal muscle and C2C12 cells.
The developmental expression of voltage-sensitive K+ channels was analyzed by Northern blot in mouse skeletal muscle. Of nine Shaker-like genes studied, eight are expressed in this mammalian muscle. Their expression is differentially regulated during development. The mouse cell line C2C12 has been used to study expression of voltage-sensitive K+ channels during in vitro myotube differentiation. Different voltage-sensitive K+ channel messages are also expressed in these cells which display a pattern of expression depending upon the differentiation stage. The message for the very peculiar K+ channel of IsK type could only be detected by polymerase chain reaction on skeletal muscle mRNA.
Lesage
F
F
Institut de Pharmacologie Moléculaire et Cellulaire, Valbonne, France.
Attali
B
B
Lazdunski
M
M
Barhanin
J
J
D000818:Animals; D015152:Blotting, Northern; D001921:Brain / Q000254:growth & development / Q000378:metabolism; D002454:Cell Differentiation; D002478:Cells, Cultured; D004247:DNA; D015342:DNA Probes; D051379:Mice; D024510:Muscle Development; D009132:Muscles / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D015221:Potassium Channels / Q000235:genetics / Q000378:metabolism*; D012313:RNA / Q000378:metabolism
D015342:DNA Probes; D015221:Potassium Channels; D012313:RNA; D004247:DNA