title: Analysis of novel imidazoles from isolated perfused rabbit heart by two high-performance liquid chromatographic methods.
abstract: The paper reports two analytical high-performance liquid chromatographic methods to detect and quantify cardiac-derived histidyl derivatives. Method A relies on relative hydrophobicities as a basis of separation. Method B is an ion-pairing method in which the compounds are eluted in an entirely different order. Fractions collected from method A have been co-eluted in admixture in method B with authentic reference compounds. Thus the existence of the following imidazole ring-containing compounds derived from heart have been confirmed: N-acetylhistidine, N-acetyl-1-methylhistidine, N-acetylcarnosine, N-acetylhomocarnosine, homocarnosine, anserine, carnosine, balenine. Compounds were found in both tissue samples and perfusates.
authors.affiliation: Institute of Physiology, Glasgow University, Scotland, UK.
authors.forename: A
authors.initials: A
authors.lastname: O'Dowd
authors.forename: J J
authors.initials: JJ
authors.lastname: O'Dowd
authors.forename: J J
authors.initials: JJ
authors.lastname: O'Dowd
authors.forename: N
authors.initials: N
authors.lastname: MacFarlane
authors.forename: H
authors.initials: H
authors.lastname: Abe
authors.forename: D J
authors.initials: DJ
authors.lastname: Miller
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D007093:Imidazoles / Q000032:analysis*; D009206:Myocardium / Q000737:chemistry*; D010477:Perfusion; D011817:Rabbits; D013056:Spectrophotometry, Ultraviolet
chemical_list: D007093:Imidazoles