Exudation of proliferative macrophages in local inflammation in the peritoneum.
Thioglycollate (TG)-elicited peritoneal macrophages (m phi s) were highly proliferative and formed m phi colonies in vitro in the presence of m phi colony-stimulating factor (M-CSF), while resident peritoneal m phi s did not. To determine whether such proliferative m phi s are immigrant or locally activated resident m phi s, mice depleted of bone marrow cells and circulating monocytes by bone-seeking radiostrontium (89Sr) were injected intraperitoneally with TG. For control (88Sr) and splenectomized (Spx) mice, more than 4 x 10(4) m phi colony-forming cells (M-CFCs) per mouse were recovered in the peritoneal lavage fluid 5 days after TG injection. 89Sr-treated mice, on the other hand, had only 20% of those in the control mice. Splenectomized and 89Sr-treated (Spx/89Sr) mice showed further depletion of bone marrow cells and monocytes and, as expected, total numbers of peritoneal M-CFCs were severely depressed to less than 1% of those in the control mice. The results suggest that levels of peritoneal M-CFCs are strongly dependent on the presence of radiosensitive bone marrow cells and circulating monocytes, and resident peritoneal m phi s activated locally by inflammatory stimuli do not form m phi colonies under the defined conditions.
Oghiso
Y
Y
Division of Comparative Radiotoxicology, National Institute of Radiological Sciences, Chiba, Japan.
Yamada
Y
Y
Shibata
Y
Y
D000818:Animals; D001853:Bone Marrow / Q000502:physiology / Q000528:radiation effects; D001854:Bone Marrow Cells; D002455:Cell Division / Q000187:drug effects; D005122:Exudates and Transudates / Q000166:cytology*; D005260:Female; D006410:Hematopoiesis / Q000528:radiation effects; D006412:Hematopoietic Stem Cells / Q000187:drug effects / Q000528:radiation effects; D016173:Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D008264:Macrophages / Q000166:cytology* / Q000187:drug effects / Q000528:radiation effects; D051379:Mice; D008809:Mice, Inbred C3H; D008954:Models, Biological; D009000:Monocytes / Q000502:physiology / Q000528:radiation effects; D010529:Peritoneal Cavity / Q000166:cytology; D010538:Peritonitis / Q000097:blood / Q000139:chemically induced / Q000473:pathology*; D011836:Radiation Tolerance; D013154:Spleen / Q000502:physiology / Q000601:surgery; D013156:Splenectomy; D013326:Strontium Radioisotopes / Q000494:pharmacology; D013864:Thioglycolates
D013326:Strontium Radioisotopes; D013864:Thioglycolates; D016173:Macrophage Colony-Stimulating Factor