Taxonomic implication of the apparent undetectability of 3-deoxy-D-manno-2-octulosonate (Kdo) in lipopolysaccharides of the representatives of the family Vibrionaceae and the occurrence of Kdo 4-phosphate in their inner-core regions.
After conventional hydrolysis of lipopolysaccharides (LPSs), Kdo was not detectable by the periodate-thiobarbituric acid test in those of any member of Vibrionaceae except the gems Plesiomonas, but phosphorylated Kdo was demonstrated after strong-acid hydrolysis. Dephosphorylation, periodate oxidation, and methylation analysis of LPS preparations from 7 strains selected from all genera of Vibrionaceae, except Plesiomonas, showed that the inner-core region (unlike that in enteric Gram-negative bacteria) contains only one molecule of Kdo 4-phosphate 5-substituted with heptose, a constituent of the distal part of the core region, as in enteric bacteria. The undetectability of Kdo in LPS after conventional hydrolysis and the occurrence of phosphorylated Kdo in strong-acid hydrolysates and of Kdo 4-phosphate in the inner-core region are taxonomic characteristics of the family Vibrionaceae.
Kondo
S
S
Department of Microbiology, Josai University, Sakado, Japan.
Haishima
Y
Y
Hisatsune
K
K
D006868:Hydrolysis; D007202:Indicators and Reagents; D008070:Lipopolysaccharides / Q000737:chemistry*; D013058:Mass Spectrometry; D008745:Methylation; D010766:Phosphorylation; D013045:Species Specificity; D013400:Sugar Acids / Q000032:analysis*; D013403:Sugar Phosphates / Q000032:analysis*; D014733:Vibrio / Q000737:chemistry / Q000145:classification*; D014737:Vibrionaceae / Q000737:chemistry / Q000145:classification*
D007202:Indicators and Reagents; D008070:Lipopolysaccharides; D013400:Sugar Acids; D013403:Sugar Phosphates; C002532:2-keto-3-deoxyoctonate; C077091:3-deoxy-2-octulosonate-4-phosphate