title: Characterization of the endogenous reverse transcriptase reaction products of SIVagmTYO-7, a simian immunodeficiency virus isolated from an African green monkey.
abstract: The products of the endogenous reverse transcriptase reaction of SIVagmTYO-7 were characterized after the reaction conditions had been optimized. The major reaction product in the presence of actinomycin D and oligo(dT) was a DNA with a size of 300 bases. Without actinomycin D two additional reaction products with 600 or 700 bases appeared. The 300 base product was identified as the (-)strong-stop DNA, whereas the 700 base product is the (+)strong-stop DNA. The 600 base product appeared only after oligo(dT) priming. The (-)strong-stop DNA hybridized specifically with a 9 kb RNA found in virus particles and three RNA species of 1.8, 4.8 and 9 kb isolated from SIVagmTYO-7 infected cells.
authors.affiliation: Deutsches Primatenzentrum, Abteilung Virologie und Immunologie, Göttingen, Germany.
authors.forename: W
authors.initials: W
authors.lastname: Lüke
authors.forename: B
authors.initials: B
authors.lastname: Dirks
authors.forename: D
authors.initials: D
authors.lastname: Moosmayer
authors.forename: K D
authors.initials: KD
authors.lastname: Jentsch
authors.forename: G
authors.initials: G
authors.lastname: Hunsmann
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D002460:Cell Line; D002522:Chlorocebus aethiops; D015345:Oligonucleotide Probes; D012367:RNA, Viral / Q000737:chemistry / Q000302:isolation & purification; D012194:RNA-Directed DNA Polymerase / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D015302:Simian Immunodeficiency Virus / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification
chemical_list: D015345:Oligonucleotide Probes; D012367:RNA, Viral; D012194:RNA-Directed DNA Polymerase