Species-specific epitopes exist on the cytoplasmic amino-terminal domain of erythrocyte band 3 protein.
Monoclonal antibodies (P3-9H, P3-1F, P3-2H, P3-4A, and P3-4C) to human erythrocyte band 3 were produced using human erythrocyte membranes as the immunogen. All epitopes defined by these antibodies were found on the amino-terminal cytoplasmic domain of erythrocyte band 3. The antibodies crossreacted variously with erythrocyte band 3 of primates (chimpanzee, orangutan, Rhesus monkey, Japanese monkey, spider monkey, and capuchin monkey) in enzyme-linked immunosorbent assay. P3-9H did not crossreact with erythrocyte band 3 of any primate examined; P3-1F crossreacted only with that of chimpanzee; P3-2H crossreacted with erythrocyte band 3 of chimpanzee, spider monkey, and capuchin monkey; and P3-4A and P3-4C crossreacted with erythrocyte band 3 of all primates examined. These results suggest that evolutional changes in primates are accumulated in the amino-terminal cytoplasmic domain of band 3 and that species-specific epitopes exist on this domain.
Kimura
A
A
Department of Legal Medicine, Wakayama Medical College.
Uda
T
T
Nakashima
S
S
Ikeda
H
H
Yasuda
S
S
Osawa
M
M
Tsuji
T
T
D000818:Animals; D001457:Anion Exchange Protein 1, Erythrocyte / Q000276:immunology* / Q000302:isolation & purification; D000911:Antibodies, Monoclonal; D000954:Antigens, Surface / Q000032:analysis; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000032:analysis*; D004910:Erythrocyte Membrane / Q000737:chemistry*; D006801:Humans; D015151:Immunoblotting; D011323:Primates / Q000276:immunology; D013045:Species Specificity
D001457:Anion Exchange Protein 1, Erythrocyte; D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D000939:Epitopes