[Use of PCR in the epidemiological identification of Campylobacter spp].
BACKGROUND
With arbitrary primer PCR technique it is possible to obtain amplification lane patterns easily and with good reproducibility from the genomic DNA of bacteria studied. There is also no need for prior information regarding the DNA sequence.


METHODS
This method implies two cycles of amplification with low stringency, followed by a PCR of high stringency.


RESULTS
Using the above mentioned technique, we were able to show that Campylobacter spp from clinical samples could be separated as well as different strains from the same species.


CONCLUSIONS
According to our results as well as the ones from different authors applied to other microorganisms, we can assume that the method could be used in any bacterial species for epidemiologic studies purposes.
Torres
M J
MJ
Departamento de Microbiología, Facultad de Medicina, Sevilla.
Palomares
J C
JC
D001483:Base Sequence; D002167:Campylobacter / Q000145:classification* / Q000302:isolation & purification; D002169:Campylobacter Infections / Q000453:epidemiology / Q000382:microbiology*; D016123:Campylobacter jejuni / Q000145:classification / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000032:analysis; D003967:Diarrhea / Q000453:epidemiology / Q000382:microbiology*; D016481:Helicobacter Infections / Q000453:epidemiology / Q000382:microbiology*; D016480:Helicobacter pylori / Q000145:classification* / Q000302:isolation & purification; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods
D004269:DNA, Bacterial