title: Effect of heat-shock on Plasmodium falciparum viability, growth and expression of the heat-shock protein 'PFHSP70-I' gene.
abstract: Cultures of the human malaria parasite Plasmodium falciparum were subjected to heat-shock for varying times and temperatures and then tested for their viability, growth and expression of heat-shock protein. Results show that the majority of parasites remained viable after heat-shock but their growth was affected. However, the expression of the heat-shock protein 'PFHSP70-I' gene was enhanced after heat-shock. We conclude that malarial parasites are able to survive in vivo during fever probably due to the overexpression of the heat-shock protein gene.
authors.affiliation: Department of Biotechnology, All India Institute of Medical Sciences, New Delhi.
authors.forename: B
authors.initials: B
authors.lastname: Joshi
authors.forename: S
authors.initials: S
authors.lastname: Biswas
authors.forename: Y D
authors.initials: YD
authors.lastname: Sharma
mesh_terms: D000818:Animals; D015870:Gene Expression; D005796:Genes; D006360:Heat-Shock Proteins / Q000235:genetics*; D006358:Hot Temperature; D007700:Kinetics; D010963:Plasmodium falciparum / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D013696:Temperature; D014158:Transcription, Genetic
chemical_list: D006360:Heat-Shock Proteins; D012313:RNA