[Production and characterization of a murine protective monoclonal antibody against Schistosoma japonicum schistosomula].
Eight murine monoclonal antibodies against surface determinants of Schistosoma japonicum (Chinese mainland strain) schistosomula were generated, of which only one monoclonal IgM antibody (N15D9) gave protection at level ranging from 14 to 39% in experiments of passive transfer or inhibition of infectivity while the others did not exhibit significant levels of passive protection. Further characterization of N15D9 antigen specificity showed that 96 and 14 kDa antigen molecules in cercaria, and 132 and 10 kDa in schistosomula could be recognized by N15D9 in Western blot assay. Furthermore, the 96 and 132 kDa molecules could also be recognized by pooled infected human sera while 14 kDa and 10 kDa only by sera from mice vaccinated with 3-hour schistosomula. The molecules recognizable by N15D9 were surface epitopes repeatedly expressed on cercaria, in vitro 3-hour mechanically transformed schistosomula and 5 day lung-stage schistosomula, as demonstrated by indirect immunofluorescence surface binding assay.
Wang
D K
DK
Department of Parasitology, Nanjing Medical College.
Wu
G L
GL
Zhao
W X
WX
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D000947:Antigens, Helminth / Q000276:immunology; D015153:Blotting, Western; D005455:Fluorescent Antibody Technique; D006825:Hybridomas / Q000378:metabolism; D007814:Larva; D051379:Mice; D008807:Mice, Inbred BALB C; D012549:Schistosoma japonicum / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth