Localization of the gene coding for ventricular myosin regulatory light chain (MYL2) to human chromosome 12q23-q24.3.
Human myosin light chain-2 (MYL2) is an important protein involved in the regulation of myosin ATPase activity in smooth muscle. In cardiac muscle, the precise role of MYL2 is not well understood; however, an increase in ventricular MYL2 is observed during myocardial hypertrophy in cardiac patients with valve stenosis. The chromosomal location of the gene coding for MYL2 was identified using a cloned cDNA for human MYL2. Southern blot analysis of DNA from a human/rodent somatic cell hybrid mapping panel showed that the BamHI fragment that hybridized with this cDNA probe was concordant with chromosome 12. The 768-bp cDNA was hybridized to human metaphase chromosomes. The results revealed a significant clustering of silver grains over chromosome 12 bands q23-q24.3, indicating that the gene coding for MYL2 is located in this region.
Macera
M J
MJ
Division of Genetics, Long Island College Hospital, Brooklyn, New York 11201.
Szabo
P
P
Wadgaonkar
R
R
Siddiqui
M A
MA
Verma
R S
RS
D006332:Cardiomegaly / Q000235:genetics / Q000378:metabolism; D002874:Chromosome Mapping; D002881:Chromosomes, Human, Pair 12; D004247:DNA / Q000235:genetics; D006352:Heart Ventricles / Q000378:metabolism; D006801:Humans; D006822:Hybrid Cells; D009218:Myosins / Q000235:genetics* / Q000378:metabolism
D004247:DNA; D009218:Myosins