Enhancement by concanavalin A of the suppressive effect of prodigiosin 25-C on proliferation of murine splenocytes.
Proliferation of concanavalin A (Con A)-activated nylon-wool purified murine splenic T cells was increasingly suppressed by prodigiosin 25-C as higher concentrations of Con A were used for the activation. Enhancement of suppressive effect of prodigiosin 25-C was not observed when T cells were stimulated with phytohemagglutinin (PHA), anti-CD3 antibody, or allogeneic splenic adherent cells. The suppressive effect of prodigiosin 25-C was enhanced by the addition of Con A in various T cell subpopulations as well as in LPS-activated splenic B cells. Lectins that recognize mannose residue of biantennary-complex-type sugar chains significantly enhanced the suppressive effect of prodigiosin 25-C, whereas a lectin that binds to N-acetylglucosamine did not. These results suggest that binding of lectins to the mannose residue of biantennary-complex-type sugar chains on cell surface of both T and B lymphocytes plays a central role on the enhancement of the suppressive effect of prodigiosin 25-C.
Kataoka
T
T
Department of Agricultural Chemistry, University of Tokyo, Japan.
Magae
J
J
Nariuchi
H
H
Yamasaki
M
M
Nagai
K
K
D000818:Animals; D002460:Cell Line; D003208:Concanavalin A; D004357:Drug Synergism; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D008213:Lymphocyte Activation / Q000187:drug effects*; D051379:Mice; D008815:Mice, Inbred Strains; D011353:Prodigiosin / Q000031:analogs & derivatives* / Q000494:pharmacology; D011948:Receptors, Antigen, T-Cell / Q000502:physiology
D007166:Immunosuppressive Agents; D011948:Receptors, Antigen, T-Cell; D003208:Concanavalin A; C077331:prodigiosin 25C; D011353:Prodigiosin