[The effect of osmotic pressure on oligomycin-sensitive ATPase activity and the liberation of Mg2+ from liver mitochondria of rats of various ages].
The influence of osmotic pressure of the incubating medium (25-500 mM sucrose) on oligomycin--sensitive, 2,4-dinitrophenyl-stimulated ATP-ase-activity, Mg2+ release and swelling of the liver mitochondria in 1-, 3-, 12-, 24-months Wistar rats is, investigated to determine age changes of structurally functional state of mitochondria. An increase in the sucrose concentration in the medium from 150 to 500 mM causes almost equal and practically absolute inhibition of ATP-ase-activity in different-age groups of rats, regardless of the presence or absence of Mg2+ ions in the medium A fall of the sucrose concentration to 150-25 mM induces a decrease in mitochondria ATP-ase-activity in Mg2+ free medium in 12- and 24-months rats (to 30 and 22%, respectively). No changes are observed in 1- and 3-months animals. Differences in rates of exogenous NADH oxidation by mitochondria of 1- and 12-months rats as a reflection of inner membrane damage degree are not observed under these conditions. Relative changes in ATP-ase-activity in a Mg2+ free medium with sucrose concentration of 25 mM (compared with 150 mM) correlate (r = 0.82) with those of optical density of mitochondria, measured at light wave length of 520 nm. It is obvious that the liver mitochondria of young and old rats sufficiently differ in spontaneous swelling rate in the media with different osmotic pressure: mitochondria of 1-month rats swell much faster than those of old rats. Considerable age differences of osmotic dependence of Mg2+ output from mitochondria are observed. They depend also on peculiarities of spontaneous organelle swelling dynamics.(ABSTRACT TRUNCATED AT 250 WORDS)
Lemeshko
V V
VV
Udovikova
E A
EA
D019297:2,4-Dinitrophenol; D000375:Aging / Q000378:metabolism*; D000818:Animals; D002413:Cations, Divalent; D004140:Dinitrophenols / Q000494:pharmacology; D004789:Enzyme Activation; D008274:Magnesium / Q000378:metabolism*; D008297:Male; D008930:Mitochondria, Liver / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D009840:Oligomycins / Q000494:pharmacology*; D009997:Osmotic Pressure; D006180:Proton-Translocating ATPases / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D002413:Cations, Divalent; D004140:Dinitrophenols; D009840:Oligomycins; D006180:Proton-Translocating ATPases; D008274:Magnesium; D019297:2,4-Dinitrophenol