Human tonsil intraepithelial B cells: a marginal zone-related subpopulation.
OBJECTIVE
To determine if intraepithelial B cells in reactive human palatine tonsils were similar to the marginal zone cells of the spleen and Peyer's patches.


METHODS
Reactive human palatine tonsils were studied using conventional methods of light microscopy, electron microscopy, and a panel of monoclonal antibodies for leucocyte common antigens.


RESULTS
Clinically important numbers of marginal zone-related B cells around the mantle zone were absent in lymphoid follicles, but in the cryptal epithelium there were abundant lymphoid cells with centrocyte-like nuclei and clear cytoplasm, intermingled with macrophages and plasma cells. The immunophenotype of these intraepithelial B cells was distinctive and similar to that found in the splenic marginal zone cells (IgM+, IgD-, CD23-, CD10-, CD35+, CD21+, bc12+, KB61+).


CONCLUSIONS
Intraepithelial B cells in human tonsil could represent the counterpart of the marginal zone described in Peyer's patches. Their presence within the epithelium could reflect the destination for the malignant B cells in the lymphoepithelial lesion of mucosa associated lymphoid tissue (MALT) lymphomas. Human palatine tonsil lymphoid tissue has morphological, immunophenotypic, and pathological features similar to those of MALT.
Morente
M
M
Department of Pathology, Hospital General de Guadalajara, Universidad de Alcalá de Henares, Spain.
Piris
M A
MA
Orradre
J L
JL
Rivas
C
C
Villuendas
R
R
D001402:B-Lymphocytes / Q000276:immunology / Q000648:ultrastructure*; D017252:CD3 Complex / Q000032:analysis; D004848:Epithelium / Q000648:ultrastructure; D006801:Humans; D007072:Immunoglobulin D / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D016130:Immunophenotyping; D007958:Leukocyte Count; D008854:Microscopy, Electron; D014066:Palatine Tonsil / Q000648:ultrastructure*
D017252:CD3 Complex; D007072:Immunoglobulin D; D007075:Immunoglobulin M