title: Basic blue 54: a new colorant for monocytes.
abstract: C.I. basic blue 54, a sulfur containing azo textile dye, stained the nucleus and cytoplasm of normal and leukemic monocytes bright red-violet. Essential for the staining reaction was a brief final rinse in a pH 3.6 acetic acid-sodium acetate buffer. Coloration of the type found in monocytes was not observed in other types of mature and immature leukocytes.
authors.affiliation: Department of Pathology, Case Western Reserve University School of Medicine, Cleveland, Ohio 44106.
authors.forename: L
authors.initials: L
authors.lastname: Kass
mesh_terms: D001391:Azo Compounds; D002843:Chromatin / Q000737:chemistry; D003593:Cytoplasm / Q000737:chemistry; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000097:blood; D007951:Leukemia, Myeloid / Q000097:blood; D015479:Leukemia, Myelomonocytic, Acute / Q000097:blood; D007962:Leukocytes / Q000166:cytology; D009000:Monocytes / Q000166:cytology*; D009504:Neutrophils / Q000166:cytology; D013194:Staining and Labeling; D013844:Thiazoles
chemical_list: D001391:Azo Compounds; D002843:Chromatin; D013844:Thiazoles; C075348:C.I. basic blue 54