Structure of HIV-1 reverse transcriptase/DNA complex at 7 A resolution showing active site locations.
AIDS, caused by human immunodeficiency virus (HIV), is one of the world's most serious health problems, with current protocols being inadequate for either prevention or successful long-term treatment. In retroviruses such as HIV, the enzyme reverse transcriptase copies the single-stranded RNA genome into double-stranded DNA that is then integrated into the chromosomes of infected cells. Reverse transcriptase is the target of the most widely used treatments for AIDS, 3'-azido-3'-deoxythymidine (AZT) and 2',3'-dideoxyinosine (ddI), but resistant strains of HIV-1 arise in patients after a relatively short time. There are several nonnucleoside inhibitors of HIV-1 reverse transcriptase, but resistance to such agents also develops rapidly. We report here the structure at 7 A resolution of a ternary complex of the HIV-1 reverse transcriptase heterodimer, a monoclonal antibody Fab fragment, and a duplex DNA template-primer. The double-stranded DNA binds in a groove on the surface of the enzyme. The electron density near one end of the DNA matches well with the known structure of the HIV-1 reverse transcriptase RNase H domain. At the opposite end of the DNA, a mercurated derivative of UTP has been localized by difference Fourier methods, allowing tentative identification of the polymerase nucleoside triphosphate binding site. We also determined the structure of the reverse transcriptase/Fab complex in the absence of template-primer to compare the bound and free forms of the enzyme. The presence of DNA correlates with movement of protein electron density in the vicinity of the putative template-primer binding groove. These results have important implications for developing improved inhibitors of reverse transcriptase for the treatment of AIDS.
Arnold
E
E
Center for Advanced Biotechnology and Medicine (CABM), Piscataway, New Jersey 08854-5638.
Jacobo-Molina
A
A
Nanni
R G
RG
Williams
R L
RL
Lu
X
X
Ding
J
J
Clark
A D
AD
Zhang
A
A
Ferris
A L
AL
Clark
P
P
D001483:Base Sequence; D001665:Binding Sites; D003460:Crystallization; D004247:DNA / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000737:chemistry*; D054303:HIV Reverse Transcriptase; D015497:HIV-1 / Q000201:enzymology*; D007140:Immunoglobulin Fab Fragments; D008767:Methylmercury Compounds; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009941:Organomercury Compounds; D011487:Protein Conformation; D012194:RNA-Directed DNA Polymerase / Q000737:chemistry*; D013329:Structure-Activity Relationship; D014544:Uridine Triphosphate / Q000031:analogs & derivatives; D014961:X-Ray Diffraction
D004268:DNA-Binding Proteins; D007140:Immunoglobulin Fab Fragments; D008767:Methylmercury Compounds; D009941:Organomercury Compounds; C016782:tetrakis(acetoxymercuri)methane; C035548:5'-mercuriuridine triphosphate; D004247:DNA; D054303:HIV Reverse Transcriptase; D012194:RNA-Directed DNA Polymerase; D014544:Uridine Triphosphate