Changes in the carbohydrate content of airway epithelium induced by human neutrophil elastase in the hamster.
Hamster airway epithelial secretory cells were investigated by light and electron microscopic cytochemistry to study possible changes in their carbohydrate content induced by human neutrophil elastase (HNE), an agent known to cause replacement of Clara cells by mucous cells in hamster bronchi. Characterization of secretory cell carbohydrates by the AB/PAS, PA-TCH-SP, HID-TCH-SP, and LID-TCH-SP sequences indicated the existence of periodate-reactive acidic glycoconjugates, but the absence of sulfated or carboxylated glycoconjugates in both treated and control animals. Differences were seen in the quality and quantity of historeactive carbohydrates throughout various regions in the lower respiratory tract. This was especially evident in the HNE-treated animals. It is concluded that the HNE-induced expression of the mucous cell phenotype is associated with an increase in the amount of neutral and acidic nonsulfated and noncarboxylated polysaccharides stored in the secretory granules of these cells.
Dimitriadis
V K
VK
Department of Anatomy, School of Veterinary Medicine, University of California, Davis.
Christensen
T G
TG
Lucey
E C
EC
Snider
G L
GL
Plopper
C G
CG
D000818:Animals; D001980:Bronchi / Q000737:chemistry* / Q000187:drug effects* / Q000648:ultrastructure; D002241:Carbohydrates / Q000032:analysis*; D006224:Cricetinae; D003594:Cytoplasmic Granules / Q000737:chemistry / Q000187:drug effects / Q000648:ultrastructure; D006801:Humans; D019272:Leukocyte Elastase; D008297:Male; D008647:Mesocricetus; D010196:Pancreatic Elastase / Q000633:toxicity*; D014132:Trachea / Q000737:chemistry / Q000187:drug effects* / Q000648:ultrastructure
D002241:Carbohydrates; D010196:Pancreatic Elastase; D019272:Leukocyte Elastase