Dark induction of haem oxygenase messenger RNA by haematoporphyrin derivative and zinc phthalocyanine; agents for photodynamic therapy.
Haematoporphyrin derivative is one of the main drugs currently used in clinical trials involving photodynamic therapy of cancer, and zinc phthalocyanine is being considered as one of several possible alternatives. We show that incubation of cultured human fibroblasts populations with either of the two drugs will lead to a sharp increase in the accumulation of the messenger RNA corresponding to haem oxygenase. Only cells incubated with haematoporphyrin derivative show additional enhancement of expression of this specific gene on exposure to red light. Since haem oxygenase induction appears to be a specific stress response that may be involved in cellular defence, such observations should be confirmed under conditions which would allow the clinical implications to be fully evaluated.
Bressoud
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Institut Suisse de Recherches Expérimentales sur le Cancer (ISREC), Epalinges Switzerland.
Jomini
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Tyrrell
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RM
D002460:Cell Line; D003624:Darkness; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D017324:Hematoporphyrin Derivative / Q000494:pharmacology*; D006419:Heme Oxygenase (Decyclizing) / Q000096:biosynthesis / Q000235:genetics*; D006801:Humans; D007211:Indoles / Q000494:pharmacology*; D054833:Isoindoles; D007700:Kinetics; D008027:Light; D009942:Organometallic Compounds / Q000494:pharmacology*; D010778:Photochemotherapy; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000235:genetics; D012867:Skin / Q000187:drug effects / Q000378:metabolism; D014158:Transcription, Genetic / Q000187:drug effects*; D015032:Zinc / Q000494:pharmacology*; D017967:Zinc Compounds
D007211:Indoles; D054833:Isoindoles; D009942:Organometallic Compounds; D012333:RNA, Messenger; D017967:Zinc Compounds; C052159:Zn(II)-phthalocyanine; D017324:Hematoporphyrin Derivative; D006419:Heme Oxygenase (Decyclizing); D015032:Zinc