Identification of two clock proteins in Acetabularia cliftonii and construction of cDNA libraries from Acetabularia cliftonii and Acetabularia mediterranea.
1. Two clock proteins were identified in A. cliftonii. The first has a molecular weight (mol. wt) of 200 kDa (P200) and its synthesis shows a 24 hr periodicity. The second has mol. wt of 130 kDa (P130) and shows a semicircadian rhythm with a periodicity of about 12 hr. 2. cDNA libraries from A. cliftonii and A. mediterranea were prepared by cloning cDNA in lambda gt10 and lambda gt11, respectively. 3. One clone each of the two libraries hybridized with the human beta-actin pseudogene. One clone of the A. mediterranea and 4 clones of the A. cliftonii libraries hybridized to Chlamydomonas heat-shock gene.
Yang
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Max-Planck-Institut für Zellbiologie, Abteilung Schweiger, Ladenburg, Fed. Rep. Germany.
de Groot
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EJ
D000076:Acetabularia / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D002940:Circadian Rhythm / Q000235:genetics*; D004247:DNA / Q000235:genetics*; D015723:Gene Library; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010940:Plant Proteins / Q000032:analysis* / Q000096:biosynthesis / Q000235:genetics; D011061:Poly A / Q000235:genetics / Q000302:isolation & purification; D014176:Protein Biosynthesis / Q000235:genetics; D012313:RNA / Q000302:isolation & purification
D010940:Plant Proteins; D011061:Poly A; D012313:RNA; D004247:DNA