Quantification of 5-fluoro-2'-deoxyuridine in plasma by gas chromatography and negative-ion chemical ionization mass spectrometry.
A sensitive and specific method using gas chromatography and negative-ion chemical ionization mass spectrometry is described for the determination of 5-fluoro-2'-deoxyuridine (FdUrd) in plasma. The method is based on the formation of the pentafluoropropionyl derivative of FdUrd and of its stable isotope as internal standard after sample clean-up by solid-phase extraction and purification by high-performance liquid chromatography. Quantification in plasma was possible down to 300 pg/ml. The method was applied to the analysis of plasma levels of FdUrd in mice and dogs.
Sato
J
J
Bio-Medical Research Laboratories, Tokyo, Japan.
Watanabe
K
K
Takahama
S
S
Makita
T
T
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004285:Dogs; D005467:Floxuridine / Q000097:blood*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C
D005467:Floxuridine