Increased resistance to the toxic effects of alkylating agents in tobacco expressing the E. coli DNA repair gene ada.
The protein coding region of the E. coli gene ada has been transferred to tobacco plants by a leaf disc transformation procedure involving an Agrobacterium tumefaciens Ti plasmid. Transformed plants were shown to be transgenic for the ada message and had increased levels of O6-alkylguanine DNA alkyltransferase activity. The N-methyl-N-nitrosourea- or taurinechlorethylnitrosourea-induced inhibition of growth of calluses or of cells in suspension was considerably lower in ada-transformed than in non-transformed plants. This indicates that O6-alkylguanine, O4-alkylthymine or phosphotriesters are growth-inhibitory lesions in tobacco.
Angelis
K
K
Institute of Experimental Botany CSAV, Prague.
Bríza
J
J
Satava
J
J
Skákal
I
I
Velemínský
J
J
Vlasák
J
J
Kleibl
K
K
Margison
G P
GP
D016960:Agrobacterium tumefaciens / Q000235:genetics; D000477:Alkylating Agents / Q000633:toxicity*; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004260:DNA Repair / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial / Q000235:genetics; D008780:Methyltransferases / Q000235:genetics*; D019853:O(6)-Methylguanine-DNA Methyltransferase; D010947:Plants, Toxic; D010957:Plasmids / Q000235:genetics; D014026:Nicotiana / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics / Q000254:growth & development; D014162:Transfection / Q000235:genetics
D000477:Alkylating Agents; D008780:Methyltransferases; D019853:O(6)-Methylguanine-DNA Methyltransferase