title: Proliferating cell nuclear antigen immunohistochemistry in rat aorta after balloon denudation. Comparison with thymidine and bromodeoxyuridine labeling.
abstract: The authors compared detection of proliferating vascular smooth muscle cells (SMC) in vitro and in vivo with proliferating cell nuclear antigen (PCNA) immunohistochemistry and two established methods: [3H]thymidine autoradiography and bromodeoxyuridine immunohistochemistry. Labeling with [3H]thymidine and bromodeoxyuridine of rat vascular SMC in culture stained 11% +/- 2% and 11% +/- 1% of cells, and PCNA immunohistochemistry 22% +/- 2% of cells. Proliferation in the media of the denuded rat aortae was highest 3 days after denudation: 4.2%, 3.8%, and 4.7% of labeled cells with [3H]thymidine, bromodeoxyuridine, and PCNA, respectively. In the intima, proliferation was highest 7 days after denudation with 42% [3H]thymidine, 40% bromodeoxyuridine, and 46% PCNA-positive cells. With double labeling, all [3H]thymidine-positive cells were PCNA positive, whereas some cells were only positive for PCNA. The authors conclude that PCNA immunohistochemistry compares favorably with [3H]thymidine autoradiography, and bromodeoxyuridine immunohistochemistry.
authors.affiliation: Division of Cardiology, Cedars Sinai Medical Center, Los Angeles, California 90048-0750.
authors.forename: U
authors.initials: U
authors.lastname: Zeymer
authors.forename: M C
authors.initials: MC
authors.lastname: Fishbein
authors.forename: J S
authors.initials: JS
authors.lastname: Forrester
authors.forename: B
authors.initials: B
authors.lastname: Cercek
mesh_terms: D000818:Animals; D001011:Aorta / Q000166:cytology / Q000378:metabolism*; D001345:Autoradiography; D001973:Bromodeoxyuridine; D002404:Catheterization; D002455:Cell Division; D002478:Cells, Cultured; D006652:Histological Techniques; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D009687:Nuclear Proteins / Q000032:analysis*; D018809:Proliferating Cell Nuclear Antigen; D051381:Rats; D011919:Rats, Inbred Strains; D013194:Staining and Labeling; D013936:Thymidine
chemical_list: D009687:Nuclear Proteins; D018809:Proliferating Cell Nuclear Antigen; D001973:Bromodeoxyuridine; D013936:Thymidine