A simplified method for the preparation of tetanus toxin binding fragment for neurobiology.
The non-toxic binding fragment of tetanus toxin (fragment C) binds avidly to neural tissue and has a growing number of neurobiological uses. Its current utility is limited by both its high commercial cost and the complex procedure for its preparation requiring highly purified tetanus toxin. We have developed a short procedure which prepares fragments of tetanus toxin from crude C. tetani extracts. The resultant proteins are atoxic with molecular sizes and immunological properties closely resembling fragment C. These proteins undergo retrograde axonal and apparent transneuronal transport in a fashion similar to fragment C.
Fishman
P S
PS
Baltimore Veterans Administration Medical Center, MD.
Farrand
D A
DA
Halpern
J L
JL
Latham
W C
WC
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000941:Antigens / Q000276:immunology; D001370:Axonal Transport; D003017:Clostridium tetani; D004591:Electrophoresis, Polyacrylamide Gel; D007002:Hypoglossal Nerve / Q000033:anatomy & histology; D015151:Immunoblotting; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D051379:Mice; D008970:Molecular Weight; D009466:Neuromuscular Blocking Agents / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism; D009474:Neurons / Q000378:metabolism; D010446:Peptide Fragments / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism; D013744:Tetanus Toxin / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism
D000911:Antibodies, Monoclonal; D000941:Antigens; D009466:Neuromuscular Blocking Agents; D010446:Peptide Fragments; D013744:Tetanus Toxin; C040635:tetanus toxin fragment C