Murine erythrocyte ankyrin cDNA: highly conserved regions of the regulatory domain.
Ankyrin is an essential link between cytoskeletal proteins, such as spectrin, and membrane bound proteins, such as protein 3, the erythrocyte anion exchanger. Although the amino acid structure of human ankyrin is known, the functional regions have been only partially defined. Sequence comparisons between mouse and human ankyrin offer one mechanism of identifying highly conserved regions that probably have functional significance. We report the isolation and sequencing of a series of overlapping murine erythroid ankyrin (Ank-1) cDNAs from spleen and reticulocyte libraries (total span 6238 bp) and identify potentially important regions of murine-human reticulocyte ankyrin homology. Comparison of the predicted peptide sequences of mouse and human erythroid ankyrins shows that these ankyrins are highly conserved in both the N-terminal, protein 3 binding domain (96% amino acid identity) and in the central spectrin-binding domain (97% identity), but differ in the C-terminal regulatory domain (79% identity). However, the C-terminal regulatory domain contains two regions of peptide sequence that are perfectly conserved. We postulate these regions are important in the regulatory functions of this domain.
White
R A
RA
Hematology/Oncology Division, Children's Hospital, Boston, Massachusetts.
Birkenmeier
C S
CS
Peters
L L
LL
Barker
J E
JE
Lux
S E
SE
D000595:Amino Acid Sequence; D000818:Animals; D017487:Ankyrins; D001483:Base Sequence / Q000235:genetics*; D001798:Blood Proteins / Q000737:chemistry / Q000235:genetics*; D015152:Blotting, Northern; D003001:Cloning, Molecular; D004247:DNA / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D004912:Erythrocytes; D006801:Humans; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
D017487:Ankyrins; D001798:Blood Proteins; D008565:Membrane Proteins; D004247:DNA