Human T cells expressing V beta 8 do not predominantly recognize DR2 alloantigen.
A panel of seven monoclonal antibodies recognizing human T-cell antigen receptor (TcR) V alpha or V beta subsets has been used to measure TcR gene expression in peripheral blood lymphocytes and mixed lymphocyte culture responses (MLR) between DR2- and DR2+ (DRw15+) donors. There were no significant differences between DR2- and DR2+ donors in per cent T cells in fresh peripheral blood labelled with any of these antibodies, which included an antibody recognizing V beta 8. This indicates strongly that increased negative selection of V beta 8+ T cells does not occur in DR2+ compared with DR2- individuals. In MLR between DR2- and DR2+ donors the only significant change compared with fresh peripheral lymphocytes was that T cells expressing V beta 5.1 were decreased in DR2- lymphocyte populations responding to DR2 alloantigen. No changes in levels of V beta 8+ T cells were detected in MLR between DR2- and DR2+ donors. This suggests that V beta 8+ T cells are not predominantly reactive against DR2 (DRw15). The data support the concept that alloreactivity against a single class II major histocompatibility complex (MHC) mismatch is mediated by T cells expressing a range of different TcR V beta molecules.
Christmas
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SE
Department of Immunology, University of Liverpool.
Brew
R
R
Crosby
I
I
Gecim
I E
IE
Sells
R A
RA
D000911:Antibodies, Monoclonal / Q000276:immunology; D002478:Cells, Cultured; D015870:Gene Expression / Q000276:immunology; D015802:HLA-DR2 Antigen / Q000276:immunology*; D006801:Humans; D007519:Isoantigens / Q000276:immunology*; D007959:Lymphocyte Culture Test, Mixed; D011948:Receptors, Antigen, T-Cell / Q000032:analysis* / Q000235:genetics; D016693:Receptors, Antigen, T-Cell, alpha-beta; D013601:T-Lymphocytes / Q000276:immunology*
D000911:Antibodies, Monoclonal; D015802:HLA-DR2 Antigen; D007519:Isoantigens; D011948:Receptors, Antigen, T-Cell; D016693:Receptors, Antigen, T-Cell, alpha-beta; C075784:T cell receptor Vbeta8