title: Lactoperoxidase-catalyzed oxidation of thiocyanate ion: a carbon-13 nuclear magnetic resonance study of the oxidation products.
abstract: Products formed from the lactoperoxidase (LPO) catalyzed oxidation of thiocyanate ion (SCN-) with hydrogen peroxide (H2O2) have been studied by 13C-NMR at pH 6 and pH 7. Ultimate formation of hypothiocyanite ion (OSCN-) as the major product correlates well with the known optical studies. The oxidation rate of SCN- appears to be greater at pH < or = 6.0. At [H2O2]/[SCN-] ratios of < or = 0.5, OSCN- is not formed immediately, but an unidentified intermediate is produced. At [H2O2]/[SCN-] > 0.5, SCN- appears to be directly oxidized to OSCN-. Once formed, OSCN- slowly degrades over a period of days to carbon dioxide (CO2), bicarbonate ion (HCO3-), and hydrogen cyanide (HCN). An additional, previously unrecognized product also appears after formation of OSCN-. On the basis of carbon-13 chemical shift information this new species is suggested to result from rearrangement of OSCN- to yield the thiooxime isomer, SCNO- or SCNOH.
authors.affiliation: Department of Chemistry, University of Iowa, Iowa City 52242.
authors.forename: J R
authors.initials: JR
authors.lastname: Pollock
authors.forename: H M
authors.initials: HM
authors.lastname: Goff
mesh_terms: D002384:Catalysis; D006863:Hydrogen-Ion Concentration; D007784:Lactoperoxidase / Q000494:pharmacology*; D009682:Magnetic Resonance Spectroscopy; D010084:Oxidation-Reduction; D013861:Thiocyanates / Q000378:metabolism*
chemical_list: D013861:Thiocyanates; D007784:Lactoperoxidase; C031760:thiocyanate