Localization of myosin IIB at the leading edge of growth cones from rat dorsal root ganglionic cells.
Primary cultures of rat dorsal root ganglionic (DRG) cells were stained with isoform-specific antibodies against non-muscle myosin II. Antibodies against the brain type myosin (MIIB) stained the peripheries of growth cones and non-neuronal cells. Double staining of the cells with the anti-myosin antibodies and rhodamine-phalloidin or anti-actin antibodies indicated that MIIB co-exists, with F-actin, at the leading edge. Antibodies against platelet myosin stained neither leading edges nor neurites, but stained the cell bodies of neurons and the stress fibers of non-neuronal cells. These results suggest that MIIB functions in the motility of the leading edge of DRG cells.
Cheng
T P
TP
Department of Anatomy and Cell Biology, SUNY, HSC, Brooklyn.
Murakami
N
N
Elzinga
M
M
D000818:Animals; D002455:Cell Division; D002478:Cells, Cultured; D005455:Fluorescent Antibody Technique; D005727:Ganglia, Spinal / Q000737:chemistry* / Q000166:cytology; D009218:Myosins / Q000032:analysis*; D016501:Neurites / Q000737:chemistry*; D009474:Neurons / Q000737:chemistry* / Q000166:cytology; D051381:Rats
D009218:Myosins