title: The influence of growth hormone on cell proliferation in odontogenic epithelia by bromodeoxyuridine immunocytochemistry and morphometry in the Lewis dwarf rat.
abstract: For investigation of how growth hormone affects tooth development, bromodeoxyuridine immunocytochemistry and morphometry were used for the study of cell proliferation in odontogenic epithelial cell layers. The number of cells in the S phase, as revealed by this technique, and in mitosis, were counted in Bouin's-perfused and paraffin-embedded undecalcified maxillary incisor enamel organs of normal rats, in growth-hormone-deficient dwarf rats, and in dwarf rats treated with growth hormone (66 micrograms/100 g body wt) twice daily for six days. Significantly fewer labeled nuclei, unlabeled nuclei, and total nuclei of various odontogenic epithelia were found in dwarf rats, but in dwarf rats treated with growth hormone, numbers of labeled nuclei equivalent to normal were found in the internal enamel epithelium, stratum intermedium, and Hertwig root sheath. Moreover, the mitotic index for pre-ameloblasts was 1.64 in normal rats, 0.92 for dwarf rats, and 1.66 for growth-hormone-treated dwarf rats (SD, 0.10). Other parameters--such as the labeling index and the ratio of positive to negative nuclei--were similarly related to GH status. Thus, growth hormone may play a role in the proliferation of the odontogenic epithelia in the rat.
authors.affiliation: Division of Oral Biology & Pathology, Faculty of Dentistry, University of Queensland, Australia.
authors.forename: W G
authors.initials: WG
authors.lastname: Young
authors.forename: C Z
authors.initials: CZ
authors.lastname: Zhang
authors.forename: H
authors.initials: H
authors.lastname: Li
authors.forename: P
authors.initials: P
authors.lastname: Osborne
authors.forename: M J
authors.initials: MJ
authors.lastname: Waters
mesh_terms: D000565:Ameloblasts / Q000166:cytology; D000566:Amelogenesis / Q000187:drug effects*; D000704:Analysis of Variance; D000818:Animals; D001973:Bromodeoxyuridine; D002455:Cell Division / Q000187:drug effects*; D004658:Enamel Organ / Q000166:cytology* / Q000187:drug effects; D004847:Epithelial Cells; D004848:Epithelium / Q000187:drug effects; D013006:Growth Hormone / Q000494:pharmacology*; D007150:Immunohistochemistry; D008297:Male; D008437:Maxilla; D008940:Mitotic Index; D009805:Odontogenesis / Q000187:drug effects*; D051381:Rats; D011917:Rats, Inbred Lew; D011994:Recombinant Proteins; D016196:S Phase
chemical_list: D011994:Recombinant Proteins; D013006:Growth Hormone; D001973:Bromodeoxyuridine