[Expression of human LSB 32/67 KD gene in E. coli and analysis of its interactions with laminin].
Earlier we have cloned cDNA coding for a polypeptide that reacts with monoclonal antibodies specific for some cytoskeleton structures. This gene is homologous to the laminin receptor 67 KD. However, cDNA suffices only for a polypeptide of 32 Da, far smaller than the 67 rDa laminin receptor. We have constructed a vector that produces the fusion protein LBP-TrpE in the bacterial strain CAG-456. Our studies show that hybrid protein LBP-TrpE is able to interact with laminin. This result was confirmed by using following methods: immunoblotting, "ELISA" and affinity chromatography on laminin.
Siianova
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D000595:Amino Acid Sequence; D002846:Chromatography, Affinity; D004270:DNA, Circular / Q000235:genetics*; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000235:genetics* / Q000254:growth & development; D005109:Extracellular Matrix; D015870:Gene Expression; D006801:Humans; D015151:Immunoblotting; D007797:Laminin / Q000032:analysis; D015514:Oncogene Proteins, Fusion / Q000235:genetics; D011946:Receptors, Antigen; D017474:Receptors, Laminin
D004270:DNA, Circular; D007797:Laminin; D015514:Oncogene Proteins, Fusion; D011946:Receptors, Antigen; D017474:Receptors, Laminin