title: Isolation of new probes from Xq12-->q13: an example of the screening of reference libraries with Alu-PCR products from radiation hybrids.
abstract: In order to isolate new probes from the juxtacentromeric region of the long arm of the human X chromosome, we used Alu-mediated polymerase chain reaction (Alu-PCR) products as probes to directly screen a chromosome X-specific gridded cosmid library. These Alu-PCR products were synthesized from radiation hybrids containing the loci DXS159, PGK1, and PGK1P1. This approach allowed us to select 18 cosmids capable of hybridizing with at least two Alu-PCR products. Four cosmids hybridized to more than three Alu-PCR products. Three of these four cosmids were contiguous, and the fourth was independent. Two cosmids that hybridized with two Alu-PCR products were further characterized. Physical mapping indicated that all of these clones are located in the expected region on Xq, confirming the validity of our approach.
authors.affiliation: U 242, Marseille, France.
authors.forename: F
authors.initials: F
authors.lastname: Muscatelli
authors.forename: A P
authors.initials: AP
authors.lastname: Monaco
authors.forename: P N
authors.initials: PN
authors.lastname: Goodfellow
authors.forename: M C
authors.initials: MC
authors.lastname: Hors-Cayla
authors.forename: H
authors.initials: H
authors.lastname: Lehrach
authors.forename: M
authors.initials: M
authors.lastname: Fontes
mesh_terms: D001483:Base Sequence; D002503:Centromere; D002871:Chromosome Banding; D002874:Chromosome Mapping; D004247:DNA / Q000235:genetics; D015723:Gene Library; D006801:Humans; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D016133:Polymerase Chain Reaction / Q000379:methods; D014960:X Chromosome
chemical_list: D009838:Oligodeoxyribonucleotides; D004247:DNA