Peritoneal exudates from microencapsulated rat islets of Langerhans xenografted mice presenting characteristics of potentially cytotoxic non-specific inflammation.
Grafted polyacrylamide microencapsulated islets of Langerhans in the peritoneal cavity of mice did not survive more than a few days, perhaps owing to a non-specific inflammatory reaction or an immune rejection. To assess the two hypotheses, we used flow cytometry (FACS) to analyse cell populations of empty or islet-loaded microcapsules grafted in the peritoneal cavity of mice, and performed cytotoxic assays with proteases secreted by inflammatory cells. An immune rejection did not seem to occur, but the degree of inflammation could explain the short life of the grafts.
Gin
H
H
INSERM U 306, Bordeaux, France.
Cadic
C
C
Baquey
C
C
Dupuy
B
B
D000180:Acrylic Resins / Q000008:administration & dosage; D000818:Animals; D003602:Cytotoxicity, Immunologic; D005122:Exudates and Transudates / Q000166:cytology; D005434:Flow Cytometry; D006084:Graft Rejection; D007249:Inflammation / Q000209:etiology*; D016381:Islets of Langerhans Transplantation / Q000379:methods*; D051379:Mice; D010529:Peritoneal Cavity / Q000166:cytology; D019736:Prostheses and Implants; D051381:Rats; D014183:Transplantation, Heterologous; D000099166:Polyacrylamides
D000180:Acrylic Resins; D000099166:Polyacrylamides