P-selectin (CD62) binds to subpopulations of human memory T lymphocytes and natural killer cells.
P-selectin (CD62) is a Ca(2+)-dependent lectin expressed on activated platelets and endothelium. Although P-selectin is known to function as a receptor for myeloid cells, previous studies indicated that P-selectin also bound to a subset of lymphocytes. Using a multi-color immunofluorescence assay we found that purified P-selectin bound to 12.2 +/- 4.1% of peripheral blood lymphocytes and that P-selectin could mediate adhesion of activated platelets to lymphocytes. A subpopulation of CD4+, CD8+, and CD16+ lymphocytes bound P-selectin. There was a marked preference for P-selectin binding to memory cells (CD45RO+) in both the CD4+ and CD8+ populations. Binding to all cell types was Ca(2+)-dependent and blocked by pretreatment of the cells with sialidase. These data suggest that P-selectin may play a role in the recruitment of specific lymphocyte populations to sites of inflammation.
Moore
K L
KL
Department of Medicine, University of Oklahoma Health Sciences Center, Oklahoma City.
Thompson
L F
LF
D015703:Antigens, CD / Q000032:analysis / Q000378:metabolism*; D001665:Binding Sites; D002118:Calcium / Q000494:pharmacology; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D004492:Edetic Acid / Q000494:pharmacology; D006801:Humans; D007156:Immunologic Memory; D007694:Killer Cells, Natural / Q000378:metabolism*; D007700:Kinetics; D008213:Lymphocyte Activation; D009439:Neuraminidase / Q000494:pharmacology; D009504:Neutrophils / Q000276:immunology / Q000378:metabolism*; D019007:P-Selectin; D010980:Platelet Membrane Glycoproteins / Q000378:metabolism*; D016176:T-Lymphocyte Subsets / Q000378:metabolism*; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism*
D015703:Antigens, CD; D015815:Cell Adhesion Molecules; D019007:P-Selectin; D010980:Platelet Membrane Glycoproteins; D004492:Edetic Acid; D009439:Neuraminidase; D002118:Calcium