title: The sequence of the gene encoding elongation factor Tu from Chlamydia trachomatis compared with those of other organisms.
abstract: Nucleotide (nt) sequences encoding the elongation factor Tu (EF-Tu), tRNA(Thr) and tRNA(Trp) from Chlamydia trachomatis have been determined. The environment of the EF-Tu-encoding gene (tuf), between two tRNA gene sequences, suggests that it is part of a tufB locus. The nt sequence and the deduced amino acid (aa) sequence were aligned with comparable sequences from other organisms and the resulting data bases were used to infer phylogenies. Phylogenetic trees based on aa sequences and nt sequences are similar, but not completely congruent with rRNA gene-based phylogenies. Both the nt and aa sequence trees concur on the early divergence of Thermotoga and Chlamydia from the bacterial root. The aa alignment highlights the presence of four unique Cys residues in the chlamydial sequence which are found at strictly conserved positions in other sequences. Further peculiarities of the chlamydial and eubacterial sequences have been mapped to the X-ray crystallographic structure of the protein.
authors.affiliation: Département de Biochimie, Université de Montréal, Québec, Canada.
authors.forename: B
authors.initials: B
authors.lastname: Cousineau
authors.forename: C
authors.initials: C
authors.lastname: Cerpa
authors.forename: J
authors.initials: J
authors.lastname: Lefebvre
authors.forename: R
authors.initials: R
authors.lastname: Cedergren
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D005075:Biological Evolution; D002692:Chlamydia trachomatis / Q000235:genetics*; D006089:Gram-Negative Anaerobic Bacteria / Q000235:genetics; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010444:Peptide Elongation Factor Tu / Q000737:chemistry / Q000235:genetics*; D010802:Phylogeny; D016415:Sequence Alignment; D017385:Sequence Homology
chemical_list: D001426:Bacterial Proteins; D010444:Peptide Elongation Factor Tu