title: Radiation hybrid map spanning the Huntington disease gene region of chromosome 4.
abstract: Radiation hybrid (RH) mapping was used to construct a map of 11 markers in the distal 4 Mb of the short arm of chromosome 4, the region containing the Huntington disease gene. Two different methods for deriving the order of the markers were compared and both arrived at the same order as being the most likely. This order is also consistent with both the physical map constructed using pulsed-field gel electrophoresis (PFGE) and the meiotic linkage map. Comparing the RH map to the map determined by PFGE provided the means to equate RH map units (centirays) with actual physical distance in kilobases of DNA. In addition, a simple procedure for reducing the complexity of human DNA in radiation hybrids is described. One cell line isolated using this procedure contains, as its only human DNA, approximately 2 Mb surrounding the Huntington disease gene.
authors.affiliation: Department of Biological Chemistry, University of California, Irvine 92717.
authors.forename: M R
authors.initials: MR
authors.lastname: Altherr
authors.forename: S
authors.initials: S
authors.lastname: Plummer
authors.forename: G
authors.initials: G
authors.lastname: Bates
authors.forename: M
authors.initials: M
authors.lastname: MacDonald
authors.forename: S
authors.initials: S
authors.lastname: Taylor
authors.forename: H
authors.initials: H
authors.lastname: Lehrach
authors.forename: A M
authors.initials: AM
authors.lastname: Frischauf
authors.forename: J F
authors.initials: JF
authors.lastname: Gusella
authors.forename: M
authors.initials: M
authors.lastname: Boehnke
authors.forename: J J
authors.initials: JJ
authors.lastname: Wasmuth
mesh_terms: D002874:Chromosome Mapping / Q000379:methods*; D002894:Chromosomes, Human, Pair 4; D005453:Fluorescence; D006801:Humans; D006816:Huntington Disease / Q000235:genetics*; D006822:Hybrid Cells / Q000528:radiation effects; D007621:Karyotyping; D009693:Nucleic Acid Hybridization