title: Influence of laminin or fibroblasts upon colony formation in the mouse by B16 melanoma cell spheroids: a morphometric analysis.
abstract: By microscopical observation and using an original morphometric method, we analyzed on histological sections the rate of lung colony formation after the intravenous injection into the mouse of B16 melanoma cells previously cultivated in vitro as aggregates. After the injection of B16 pure spheroids, superficial lung colonies were more numerous than internal lung colonies. After the injection of mixed spheroids (B16 + 3T3 fibroblasts), the size of colony sections was increased. Addition of laminin to pure or mixed spheroids decreased the size of colony sections but increased the number of internal lung colonies.
authors.affiliation: Environment Institute, Ispra, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Coucke
authors.forename: L
authors.initials: L
authors.lastname: De Leval
authors.forename: P
authors.initials: P
authors.lastname: Leyh
authors.forename: K
authors.initials: K
authors.lastname: Bonjean
authors.forename: B
authors.initials: B
authors.lastname: Siwek
authors.forename: A
authors.initials: A
authors.lastname: Noel
authors.forename: M C
authors.initials: MC
authors.lastname: De Pauw-Gillet
authors.forename: J M
authors.initials: JM
authors.lastname: Paulus
authors.forename: R
authors.initials: R
authors.lastname: Bassleer
authors.forename: J M
authors.initials: JM
authors.lastname: Foidart
mesh_terms: D016475:3T3 Cells / Q000502:physiology*; D000818:Animals; D002450:Cell Communication; D002455:Cell Division; D046508:Culture Techniques / Q000379:methods; D007797:Laminin / Q000494:pharmacology*; D008175:Lung Neoplasms / Q000098:blood supply / Q000473:pathology / Q000556:secondary*; D008546:Melanoma, Experimental / Q000098:blood supply / Q000473:pathology / Q000556:secondary*; D051379:Mice; D009361:Neoplasm Invasiveness; D009368:Neoplasm Transplantation; D009389:Neovascularization, Pathologic; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000473:pathology / Q000637:transplantation
chemical_list: D007797:Laminin