Rapid identification of legionellae by a colony blot assay based on a genus-specific monoclonal antibody.
We recently developed a monoclonal antibody immunoglobulin G2a (2125) recognizing a genus-specific epitope on the 60-kDa heat shock protein of all Legionella species. In the current study, this antibody was used in a colony blot enzyme-linked immunosorbent assay for the rapid identification of Legionella cultures on agar plates. The whole protocol was completed in less than 2 h. All 59 Legionella species and serogroups that were tested gave a positive signal. No unspecific reactions with nonlegionellae were observed. This test is a rapid procedure for the identification of legionellae growing on agar medium to the genus level.
Steinmetz
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I
Institute of Medical Microbiology, Hannover Medical School, Germany.
Rheinheimer
C
C
Bitter-Suermann
D
D
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D001431:Bacteriological Techniques; D000939:Epitopes; D005069:Evaluation Studies as Topic; D006360:Heat-Shock Proteins / Q000276:immunology; D006801:Humans; D007875:Legionella / Q000254:growth & development / Q000276:immunology / Q000302:isolation & purification*; D013045:Species Specificity
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D000939:Epitopes; D006360:Heat-Shock Proteins