cDNA cloning and gene expression of the major prolamins of rice.
A full-length cDNA (pS 18) encoding the 16 kDa rice prolamin composed of 158 amino acids was sequenced. Analysis of N-terminal amino acid sequence of a major rice prolamin indicated that an 18 amino acid signal peptide was removed from 16 kDa precursor prolamin to form the 14 kDa prolamin during seed development. Synthesis of the 16 kDa precursor prolamin began around 8 days after flowering (DAF), increased remarkably at 8-11 DAF and gradually reached maximum levels with the maturation of rice seeds.
Shyur
L F
LF
Graduate Institute of Agricultural Chemistry, National Taiwan University, Taipei.
Wen
T N
TN
Chen
C S
CS
D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D004247:DNA; D015870:Gene Expression; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012275:Oryza / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics*; D055315:Prolamins; D011506:Proteins / Q000235:genetics*; D017385:Sequence Homology
D010940:Plant Proteins; D055315:Prolamins; D011506:Proteins; D004247:DNA