[Inhibition of total and active E-rosettes mediated by serum from tubercular patients].
OBJECTIVE
To study the effect of tuberculous patients' serum upon normal lymphocytes in order to determine possible inhibition factors of immune response.


METHODS
We studied total E-rosette formation (4 degrees C, RET) and active rosette formation (37 degrees C, REA) in tuberculous patients in different stages: mild (n:36), moderate (n:28), severe (n:24) and a control group of 32 patients. The same determinations were studied in normal circulating lymphocytes (LN) incubated before with serum from tuberculous patients (SP), serum of normal individuals (SN) and in Tc-199 medium.


RESULTS
The number of RET and REA in tuberculous patients of different stages were significantly lower to the ones of the control group (p less than 0.01, p less than 0.05). This differences were also found when we compare the number of RET and REA after the incubation of LN with SP and SN respectively (p less than 0.01, p less than 0.05).


CONCLUSIONS
Tuberculous patients showed an impaired ability to form RET and REA. The incubation of normal lymphocytes with serum from tuberculous patients reduced significantly its capacity to form rosettes.
Dlugovitzky
D
D
Cátedra de Microbiología, Parasitología y Virología, Facultad de Ciencias Médicas, Universidad Nacional de Rosario.
Luchessi
S
S
Romano
B
B
Molteni
O A
OA
D000293:Adolescent; D000328:Adult; D000368:Aged; D001769:Blood / Q000276:immunology; D005260:Female; D006801:Humans; D007108:Immune Tolerance; D007153:Immunologic Deficiency Syndromes / Q000097:blood / Q000209:etiology; D008297:Male; D008875:Middle Aged; D000091402:Prohibitins; D012397:Rosette Formation; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D014397:Tuberculosis, Pulmonary / Q000097:blood* / Q000150:complications / Q000276:immunology