Mouse thromboxane A2 receptor: cDNA cloning, expression and northern blot analysis.
A cDNA clone for the mouse thromboxane A2 receptor was isolated from mouse lung cDNA library. The cDNA has a 1,023 base pair open reading frame which encodes a protein of 341 amino acid residues. STA2 and U-46619 induced inward current in Xenopus laevis oocytes injected with the transcript of the clone. Specific binding of [3H]S-145 was found in membranes of COS-1 cells transfected with the cDNA (Kd = 3.3 nM) and was displaced with unlabeled prostaglandins and thromboxane analogues in the order of S-145 greater than STA2 greater than U-46619 greater than PGD2 greater than PGF2 alpha = PGE2. Northern blot analysis demonstrated that thromboxane A2 receptor mRNA is expressed abundantly in thymus, spleen and lung.
Namba
T
T
Department of Pharmacology, Faculty of Medicine, Kyoto University, Japan.
Sugimoto
Y
Y
Hirata
M
M
Hayashi
Y
Y
Honda
A
A
Watabe
A
A
Negishi
M
M
Ichikawa
A
A
Narumiya
S
S
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001667:Binding, Competitive; D015152:Blotting, Northern; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D003001:Cloning, Molecular / Q000379:methods; D004247:DNA / Q000235:genetics*; D015723:Gene Library; D006801:Humans; D007700:Kinetics; D008168:Lung / Q000502:physiology*; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D009865:Oocytes / Q000502:physiology; D009928:Organ Specificity; D011061:Poly A / Q000235:genetics / Q000302:isolation & purification; D016133:Polymerase Chain Reaction / Q000379:methods; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012333:RNA, Messenger; D011982:Receptors, Prostaglandin / Q000235:genetics* / Q000378:metabolism; D017482:Receptors, Thromboxane; D011994:Recombinant Proteins / Q000378:metabolism; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D013928:Thromboxane A2 / Q000378:metabolism; D014158:Transcription, Genetic; D014162:Transfection; D014981:Xenopus
D009838:Oligodeoxyribonucleotides; D012333:RNA, Messenger; D011982:Receptors, Prostaglandin; D017482:Receptors, Thromboxane; D011994:Recombinant Proteins; D011061:Poly A; D013928:Thromboxane A2; D012313:RNA; D004247:DNA