Analysis of prostatic tumor cultures using fluorescence in-situ hybridization (FISH).
Analysis of ten primary prostatic tumor cultures using fluorescence in-situ hybridization (FISH) with pericentromeric probes for chromosomes 7, 8, 10, 16, 17, and 18 revealed aneusomies in nine of these specimens. Classical cytogenetics by G-banding indicated that only four of those same ten specimens had any (but not consistent) clonal abnormalities. This preliminary study suggests that aneusomy is a common event in early-stage prostatic tumors, and also supports the notion that multiple chromosomes are involved. In combination with routine cytogenetic analysis, FISH is thus likely to be a powerful tool in the evaluation of prostatic cancer.
Brothman
A R
AR
Department of Pediatrics, Eastern Virginia Medical School, Norfolk.
Patel
A M
AM
Peehl
D M
DM
Schellhammer
P F
PF
D002869:Chromosome Aberrations; D046508:Culture Techniques; D005069:Evaluation Studies as Topic; D005453:Fluorescence; D006801:Humans; D017403:In Situ Hybridization; D008297:Male; D011471:Prostatic Neoplasms / Q000235:genetics*