Hepatitis C viremia and anti-HCV antibodies in alcoholics.
We determined serum hepatitis C status using a RIBA2 kit and a sensitive PCR procedure in 62 chronic alcoholics, 36 of whom had anti-HCV antibodies (Ab) detectable in an ELISA1 assay. Anti-HCV antibodies were detected in 22 patients using RIBA2. HCV RNA was detected by means of PCR in 18 patients who were RIBA2 positive and in none who were RIBA2 negative. Liver biopsies, available for 12 HCV RNA-positive patients, revealed histological features of purely alcohol-related lesions in seven and mixed alcohol-viral lesions in five. These results indicate that HCV replication is maintained in most alcoholics who score positive for anti-HCV Ab in the RIBA2 test, and that HCV viremia can be associated with histological features typical of alcoholic liver disease.
Nalpas
B
B
Unité d'Hépatologie, Hôpital Laënnec, Paris, France.
Thiers
V
V
Pol
S
S
Driss
F
F
Thepot
V
V
Berthelot
P
P
Brechot
C
C
D000328:Adult; D000437:Alcoholism / Q000150:complications / Q000382:microbiology* / Q000473:pathology; D001483:Base Sequence; D001706:Biopsy; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D016174:Hepacivirus / Q000235:genetics / Q000302:isolation & purification*; D006508:Hepatitis Antibodies / Q000032:analysis*; D006526:Hepatitis C / Q000150:complications* / Q000473:pathology; D018937:Hepatitis C Antibodies; D006801:Humans; D008099:Liver / Q000382:microbiology* / Q000473:pathology; D008104:Liver Cirrhosis, Alcoholic / Q000382:microbiology* / Q000473:pathology; D008297:Male; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000032:analysis / Q000235:genetics; D014766:Viremia / Q000150:complications* / Q000473:pathology
D004279:DNA, Viral; D006508:Hepatitis Antibodies; D018937:Hepatitis C Antibodies; D009838:Oligodeoxyribonucleotides; D015345:Oligonucleotide Probes; D012367:RNA, Viral