A heparin-binding protein in porcine seminal plasma stimulates neurite outgrowth on neuroblastoma cells in culture.
A protein of neurite outgrowth activity has been identified in porcine seminal plasma after ammonium sulfate precipitation and affinity chromatography on heparin-Sepharose. Upon SDS-PAGE, the polypeptide is shown to have a M(r) of 16,000-18,000. Biologically by induction of neuritic processes on neuroblastoma cells, and immunologically by cross-reaction with specific antisera, this seminal plasma protein differs from acidic fibroblast growth factor (aFGF), basic fibroblast growth factor (bFGF) and nerve growth factor (NGF). The neurite outgrowth activity is relatively stable at pH 3-7 and under denaturing conditions of 8 M urea and beta-mercaptoethanol, but is inactivated by treatment of trypsin. This appears to be a novel protein, enhancing morphological differentiation of neuroblastoma cells in culture.
Cheng
K W
KW
Department of Physiology, University of Manitoba, Winnipeg, Canada.
D000818:Animals; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004305:Dose-Response Relationship, Drug; D004797:Enzyme-Linked Immunosorbent Assay; D016220:Fibroblast Growth Factor 1 / Q000378:metabolism*; D009414:Nerve Growth Factors / Q000378:metabolism; D016501:Neurites / Q000187:drug effects / Q000502:physiology*; D009447:Neuroblastoma / Q000473:pathology / Q000503:physiopathology* / Q000648:ultrastructure; D012661:Semen / Q000378:metabolism*; D013552:Swine; D014407:Tumor Cells, Cultured
D009414:Nerve Growth Factors; D016220:Fibroblast Growth Factor 1