title: Quantification of 5-fluoro-2'-deoxyuridine in plasma by gas chromatography and negative-ion chemical ionization mass spectrometry.
abstract: A sensitive and specific method using gas chromatography and negative-ion chemical ionization mass spectrometry is described for the determination of 5-fluoro-2'-deoxyuridine (FdUrd) in plasma. The method is based on the formation of the pentafluoropropionyl derivative of FdUrd and of its stable isotope as internal standard after sample clean-up by solid-phase extraction and purification by high-performance liquid chromatography. Quantification in plasma was possible down to 300 pg/ml. The method was applied to the analysis of plasma levels of FdUrd in mice and dogs.
authors.affiliation: Bio-Medical Research Laboratories, Tokyo, Japan.
authors.forename: J
authors.initials: J
authors.lastname: Sato
authors.forename: K
authors.initials: K
authors.lastname: Watanabe
authors.forename: S
authors.initials: S
authors.lastname: Takahama
authors.forename: T
authors.initials: T
authors.lastname: Makita
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004285:Dogs; D005467:Floxuridine / Q000097:blood*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C
chemical_list: D005467:Floxuridine