Study of unscheduled DNA synthesis following exposure of human cells to arecoline and extracts of betel nut in vitro.
Aqueous, acetic acid, hydrochloric acid and ethanol extracts of betel nut (Areca catechu L.) have been found to induce unscheduled DNA synthesis in Hep 2 cells obtained from human larynx carcinoma, in vitro. Different concentrations of extracts of betel nut induced dose-dependent unscheduled DNA synthesis in Hep 2 cells. Together with the viability of the Hep 2 cells, our results indicate that the aqueous and acetic acid extracts of betel nut induce relatively more unscheduled DNA synthesis than the hydrochloric acid and ethanol extracts and arecoline. The carcinogenic potency of raw and unprocessed betel nut of North-East India used in this study is discussed.
Sharan
R N
RN
Department of Biochemistry, School of Life Sciences, North Eastern Hill University, Shillong, India.
Wary
K K
KK
D001115:Arecoline / Q000633:toxicity*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004247:DNA / Q000096:biosynthesis* / Q000187:drug effects; D004249:DNA Damage; D006801:Humans; D009754:Nuts
D001115:Arecoline; D004247:DNA