Choline acetyltransferase expression studied with an oligonucleotide probe.
1. The localization of choline acetyltransferase messenger RNA has been studied using a digoxigenin-tailed complementary oligodeoxynucleotide probe for in situ hybridization. 2. Putative cholinergic cells of the rat and ferret spinal cord and the ferret retina were labeled. 3. This technique affords superior resolution compared to radioactively labeled probes, with apparently equal sensitivity.
Kennedy
R E
RE
Department of Anatomy, University of Mississippi Medical Center, Jackson 39216-4505.
Hutchins
J B
JB
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002795:Choline O-Acetyltransferase / Q000096:biosynthesis*; D004076:Digoxigenin; D005136:Eye Proteins / Q000096:biosynthesis*; D005289:Ferrets; D015870:Gene Expression; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D009046:Motor Neurons / Q000201:enzymology; D009419:Nerve Tissue Proteins / Q000096:biosynthesis*; D015345:Oligonucleotide Probes; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D012160:Retina / Q000201:enzymology*; D013116:Spinal Cord / Q000201:enzymology*
D005136:Eye Proteins; D009419:Nerve Tissue Proteins; D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D002795:Choline O-Acetyltransferase; D004076:Digoxigenin