Abnormal cerebellar output in rats with an inherited movement disorder.
Biochemical and metabolic mapping techniques have consistently identified the deep cerebellar nuclei (DCN) of the genetically dystonic rat as a site of abnormality. Extracellular single-unit recording techniques were used to assess the functional significance of these findings in affected rats and normal littermates between 16 and 25 days of age. Cells in the medial nucleus of the mutant rats had significantly increased spontaneous firing rates in comparison with cells from normal rats. In both the medial and the interpositus nuclei, cells from the mutants fired more rhythmically than those from the normal rats. When harmaline was administered systemically to activate the olivo-cerebellar system, in normal rats, increased firing rate and bursting patterns of activity were seen. There was no reliable change in the average firing rate or rhythmicity of cells in the medial nucleus of the dystonic rats, although previous studies have shown that harmaline activates neurons in the inferior olive in the mutants. It is likely that naturally stimulated olivary activity also fails to modulate cerebellar output in this model of inherited movement disorder. Anatomical studies did not reveal any consistent changes in the number of Purkinje cells, the volume of the DCN, or the soma size of DCN neurons. Since the electrophysiological findings cannot be ascribed to a loss of the Purkinje cells that normally provide an inhibitory input to the cerebellar nuclei, the results of this study indicate the presence of a functional defect in the control of cerebellar output in the dystonic rat that accounts for the failure of these animals to display harmaline tremor and which may be critical to the motor syndrome.
Lorden
J F
JF
Department of Psychology, University of Alabama, Birmingham 35294.
Lutes
J
J
Michela
V L
VL
Ervin
J
J
D000818:Animals; D002452:Cell Count; D002529:Cerebellar Nuclei / Q000187:drug effects / Q000473:pathology / Q000503:physiopathology*; D004421:Dystonia / Q000235:genetics / Q000473:pathology / Q000503:physiopathology*; D004594:Electrophysiology; D005260:Female; D006246:Harmaline / Q000494:pharmacology; D008297:Male; D010641:Phenotype; D011689:Purkinje Cells / Q000187:drug effects / Q000502:physiology; D051381:Rats; D011922:Rats, Mutant Strains; D012016:Reference Values
D006246:Harmaline