Effect of neurotransmitters on axoplasmic transport: acetylcholine effect on superior cervical ganglion cells.
The effect of acetylcholine (ACh) on particle movements along axons of cultured superior cervical ganglion cells was analyzed with a computer-assisted video-enhanced differential interference contrast microscope system. ACh suppressed the axoplasmic transport reversibly in both anterograde and retrograde directions. A muscarinic agonist, arecoline, mimicked the ACh effect, but nicotine did not. An experiment with the Ca(2+)-indicator dye, fura-2, revealed that ACh suppressed the transport without any change of intracellular Ca2+ concentration. ACh also suppressed the axoplasmic transport in Ca(2+)-free medium. Islet-activating protein (IAP), pertussis toxin, blocked the ACh effect. These results indicate that ACh activates muscarinic receptors and suppresses fast axoplasmic transport through the activation of IAP-sensitive GTP-binding protein, irrespective of Ca2+ ions.
Takenaka
T
T
Department of Physiology, Yokohama City University, Japan.
Kawakami
T
T
Hikawa
N
N
Bandou
Y
Y
Gotoh
H
H
D000109:Acetylcholine / Q000494:pharmacology*; D000818:Animals; D001370:Axonal Transport / Q000187:drug effects*; D002118:Calcium / Q000378:metabolism; D046508:Culture Techniques; D019204:GTP-Binding Proteins / Q000378:metabolism; D005728:Ganglia, Sympathetic / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018377:Neurotransmitter Agents / Q000502:physiology*; D037342:Pertussis Toxin; D011976:Receptors, Muscarinic / Q000187:drug effects; D010566:Virulence Factors, Bordetella / Q000494:pharmacology
D018377:Neurotransmitter Agents; D011976:Receptors, Muscarinic; D010566:Virulence Factors, Bordetella; D037342:Pertussis Toxin; D019204:GTP-Binding Proteins; D000109:Acetylcholine; D002118:Calcium