Immunologic identification of Na/Ca exchange protein in rat brain synaptic plasma membrane.
Polyclonal antibodies raised against partially purified dog cardiac Na/Ca exchanger react with cardiac sarcolemmal proteins of 160, 120 and 70 kDa on SDS-PAGE. Using the same specific antiserum, we detected three prominent immunoreactive bands of about 150, 120 and 70 kDa on immunoblots with rat forebrain synaptic plasma membrane proteins. These data indicate that the Na/Ca exchange protein in rat brain synaptic plasma membrane is structurally and antigenically similar to the exchange protein in dog cardiac sarcolemma.
Yip
R K
RK
Department of Physiology, University of Maryland School of Medicine, Baltimore 21201.
Blaustein
M P
MP
Philipson
K D
KD
D000818:Animals; D000918:Antibody Specificity; D002352:Carrier Proteins / Q000032:analysis* / Q000276:immunology; D003429:Cross Reactions; D004285:Dogs; D000939:Epitopes / Q000276:immunology; D008565:Membrane Proteins / Q000032:analysis* / Q000276:immunology; D016548:Prosencephalon / Q000737:chemistry*; D051381:Rats; D012508:Sarcolemma / Q000737:chemistry; D019831:Sodium-Calcium Exchanger; D013570:Synaptic Membranes / Q000737:chemistry*
D002352:Carrier Proteins; D000939:Epitopes; D008565:Membrane Proteins; D019831:Sodium-Calcium Exchanger