title: Localization of allergens in the domestic mite Lepidoglyphus destructor.
abstract: The non-pyroglyphid domestic mite Lepidoglyphus destructor is a major source of allergen causing respiratory symptoms in farming environments. This study is the first to focus on the localization of the allergens in the non-pyroglyphid domestic mite Lepidoglyphus destructor. Cryostat-cut sections of L. destructor mite bodies and faecal pellets were probed with one of three mouse monoclonal antibodies (MoAbs) raised against L. destructor or with patient sera, and stained with immunoperoxidase. Eight sera were RAST-positive to L. destructor and L. destructor faecal pellets. These eight RAST-positive patient sera labelled the wall of the L. destructor gut and two of them also the faecal pellets. The MoAbs additionally labelled parts of the exoskeleton and reacted with a majority of the faecal pellets. Cryostat-cut sections of Dermatophagoides pteronyssinus mite bodies and faecal pellets were probed with L. destructor MoAbs, which resulted in only slight staining of a few faecal pellets. The results suggest that at least one L. destructor allergen is associated with digestion.
authors.affiliation: Department of Clinical Immunology, Karolinska Institute, Karolinska Hospital, Stockholm, Sweden.
authors.forename: M
authors.initials: M
authors.lastname: van Hage-Hamsten
authors.forename: A
authors.initials: A
authors.lastname: Scheynius
authors.forename: B
authors.initials: B
authors.lastname: Härfast
authors.forename: A
authors.initials: A
authors.lastname: Wirén
authors.forename: S G
authors.initials: SG
authors.lastname: Johansson
mesh_terms: D000328:Adult; D000368:Aged; D000383:Agriculture; D000485:Allergens / Q000032:analysis*; D000818:Animals; D000911:Antibodies, Monoclonal; D006801:Humans; D006967:Hypersensitivity / Q000276:immunology; D007124:Immunoenzyme Techniques; D008875:Middle Aged; D008925:Mites / Q000276:immunology*; D011852:Radioallergosorbent Test; D013194:Staining and Labeling; D014018:Tissue Distribution
chemical_list: D000485:Allergens; D000911:Antibodies, Monoclonal