Time-saving in biological dosimetry by using the automatic metaphase finder Metafer2.
The amount of time-saving by using the Metafer2 metaphase finder for routine analysis of radiation-induced chromosome aberrations (biological dosimetry) was determined. Metaphases were prepared by standard methods from cultures of human peripheral blood lymphocytes and stained either with Giemsa or with the FPG method. The metaphase finder was used for detecting metaphases on the microscope slides and for automatically processing the evaluation data. In our laboratory, standardized analysis of 1000 metaphases requires at least 3 working days for cell culturing and slide preparation and 51.5 working hours for cytogenetic analysis. When using the metaphase finder the time required for cytogenetic analysis is reduced to 17.3 working hours (time-saving factor: 51.5/17.3 h = 3.0). In our prolonged method, including more than one scoring of each slide and karyotyping of metaphases with chromosome aberrations, the analysis times for 1000 cells are 132 and 70 working hours, respectively (time saving factor: 132/70 h = 1.9).
Weber
J
J
Institute of Radiation Biology, University of Münster, Germany.
Scheid
W
W
Traut
H
H
D001331:Automation; D015197:Carcinogenicity Tests; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D003582:Cytogenetics / Q000379:methods*; D006801:Humans; D008214:Lymphocytes / Q000528:radiation effects*; D008677:Metaphase / Q000235:genetics*; D008853:Microscopy; D009152:Mutagenicity Tests