title: Heme oxygenase is a heat shock protein and PEST protein in rat astroglial cells.
abstract: Cultured rat forebrain astrocytes contained significant amounts of immunostainable heme oxygenase-1 (HO-1) isozyme, whereas HO-1 was undetectable in spontaneously transformed rat astroglial cells (ATs). HO-1 was inducible in both cell types by heat shock and by submicromolar amounts of H2O2. Inhibition of RNA synthesis with actinomycin D or protein synthesis with cycloheximide resulted in the rapid loss of immunostainable heme oxygenase in astrocytes. Analysis of the primary structure of heme oxygenase suggests that it is a PEST protein, i.e., targeted for rapid turnover.
authors.affiliation: Molecular Neurobiology Laboratory, Veterans Affairs Medical Center, Sepulveda 91343.
authors.forename: B E
authors.initials: BE
authors.lastname: Dwyer
authors.forename: R N
authors.initials: RN
authors.lastname: Nishimura
authors.forename: J
authors.initials: J
authors.lastname: De Vellis
authors.forename: T
authors.initials: T
authors.lastname: Yoshida
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000187:drug effects / Q000201:enzymology*; D001483:Base Sequence; D015152:Blotting, Northern; D015153:Blotting, Western; D002461:Cell Line, Transformed; D003513:Cycloheximide / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D015971:Gene Expression Regulation, Enzymologic; D006360:Heat-Shock Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification; D006419:Heme Oxygenase (Decyclizing) / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification; D006358:Hot Temperature; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D007527:Isoenzymes / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D016376:Oligonucleotides, Antisense; D016548:Prosencephalon / Q000201:enzymology*; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D014158:Transcription, Genetic / Q000187:drug effects
chemical_list: D006360:Heat-Shock Proteins; D007527:Isoenzymes; D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger; D003609:Dactinomycin; D012313:RNA; D003513:Cycloheximide; D006861:Hydrogen Peroxide; D006419:Heme Oxygenase (Decyclizing)