title: Homeostasis as regulated by activated macrophage. VIII. LPSw (a lipopolysaccharide from wheat flour) can regulate bone resorption of chick embryo.
abstract: The effect of LPSw (a lipopolysaccharide from wheat flour) on the bone resorption of 18-d chick embryonic calvaria was examined in an organ culture following the method of Raisz. Bone was prelabeled in culture medium containing 45Ca and chased in a cold medium. On addition of test samples, labeled calcium was released indicating the grade of bone resorption. LPSw (10-100 ng/ml) stimulated bone resorption, showing an effect comparable to parathyroid hormone (PTH) (1 U/ml). PTH at 1 U/ml decreased the total amount of calcium and phosphorus, while LPSw did not. LPSw is thus assumed to stimulate bone resorption more actively than PTH.
authors.affiliation: Department of Physiological Chemistry, Faculty of Pharmaceutical Sciences, Teikyo University, Kanagawa, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Kawashima
authors.forename: H
authors.initials: H
authors.lastname: Endo
authors.forename: T
authors.initials: T
authors.lastname: Nishizawa
authors.forename: H
authors.initials: H
authors.lastname: Inagawa
authors.forename: T
authors.initials: T
authors.lastname: Okutomi
authors.forename: A
authors.initials: A
authors.lastname: Morikawa
authors.forename: G
authors.initials: G
authors.lastname: Soma
authors.forename: D
authors.initials: D
authors.lastname: Mizuno
mesh_terms: D000818:Animals; D001862:Bone Resorption / Q000188:drug therapy* / Q000503:physiopathology; D002642:Chick Embryo; D006706:Homeostasis / Q000187:drug effects / Q000502:physiology*; D008070:Lipopolysaccharides / Q000627:therapeutic use*; D008262:Macrophage Activation / Q000187:drug effects / Q000502:physiology*; D008264:Macrophages / Q000187:drug effects / Q000502:physiology; D009924:Organ Culture Techniques; D012886:Skull / Q000196:embryology / Q000503:physiopathology; D014908:Triticum
chemical_list: D008070:Lipopolysaccharides