Progesterone metabolism in vitro in the decapod crustacean, Penaeus monodon.
Thin-layer chromatography (TLC) and reversed-phase high-performance liquid chromatography (RP-HPLC) with on-line detection of radioactive steroids were applied to identify metabolites of [4-14C]progesterone incubated in vitro with prawn ovary. There was extensive metabolism of progesterone by stage II (vitellogenic) ovary of Penaeus monodon. The most abundant metabolites were 5 alpha-pregnane derivatives together with two minor metabolites, 20 alpha-hydroxypregn-4-en-3-one and 1,4-pregnadiene-3,20-dione. In contrast, a much lower level of progesterone metabolism was observed in stage 0 (immature) ovary of this species. The hepatopancreas, gill, and abdominal muscle of P. monodon all metabolised [4-14C]progesterone to varying degrees, generating materials similar to those produced by the ovary. A comparative study of progesterone metabolism in stage II ovary of Nephrops norvegicus indicated that one metabolite, 20 alpha-hydroxypregn-4-en-3-one, was produced.
Young
N J
NJ
Department of Biochemistry, University of Liverpool, United Kingdom.
Quinlan
P T
PT
Goad
L J
LJ
D000009:Abdominal Muscles / Q000378:metabolism; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D005260:Female; D005880:Gills / Q000378:metabolism; D066298:In Vitro Techniques; D008099:Liver / Q000378:metabolism; D010053:Ovary / Q000378:metabolism; D033561:Penaeidae / Q000378:metabolism*; D011374:Progesterone / Q000378:metabolism*; D013997:Time Factors
D011374:Progesterone