Analysis of inter-serotypic structural relationships of infectious bursal disease virus using detergent solubilization and radioimmunoprecipitation with monoclonal antibodies.
Monoclonal antibodies (MAbs) neutralizing only the infectious bursal disease virus strains (IBDV) belonging to serotype 1 also immunoprecipitated the heterologous major antigenic proteins of serotype 2 IBDV. Detergent-solubilization followed by radioimmunoprecipitation assays (RIPA) using the MAbs revealed structural similarities between the conformation-dependent antigenic determinants of IBDV of the two existing serotypes. The presence of non-ionic detergent Triton X-100 determined the binding of altered proteins by MAbs in RIPA.
Reddy
S K
SK
Section Virologie, Faculté de médecine vétérinaire, Université de Montréal, St-Hyacinthe, Québec, Canada.
Silim
A
A
Frenette
D
D
D000818:Animals; D000911:Antibodies, Monoclonal; D003902:Detergents; D000939:Epitopes / Q000737:chemistry; D007243:Infectious bursal disease virus / Q000145:classification*; D015531:Radioimmunoprecipitation Assay; D012703:Serotyping; D012995:Solubility; D014709:Vero Cells
D000911:Antibodies, Monoclonal; D003902:Detergents; D000939:Epitopes