Analytical methods for the study of liver cell proliferation.
Various cytometric methods for analysis of regenerating rat liver growth (DNA ploidy distributions, binucleation, and DNA synthesis by in vivo BrdUrd incorporation) were evaluated. The overall hepatocellular growth rate (labeling index), the binucleation rate, and separate indices for mononuclear and binuclear cells could be measured simply by microscope counting of collagenase-isolated hepatocytes immunostained for BrdUrd. Flow cytometry of cells stained for BrdUrd and DNA provided labeling indices for the various hepatocellular DNA ploidy classes as well as for nonparenchymal cells (identified by their size-dependent light scatter), but could not distinguish between mononuclear and binuclear hepatocytes. Image cytometry, using fluorescence or Feulgen staining, was inferior to flow cytometry in terms of speed and DNA resolution, but allowed a complete analysis of all hepatocellular DNA ploidy and nuclearity classes. It may therefore be the method of choice, particularly for analysis of liver cell cultures from which single cells are not easily obtained. Fluorescence staining would seem to be preferable to Feulgen staining, since the latter could not be used simultaneously with BrdUrd staining and therefore required a two-step analysis. A non-immunological method, based on the ability of incorporated BrdUrd to quench DNA staining by a Hoechst dye, could only be applied to isolated nuclei, thus giving no information about binucleation. The latter method may be useful for analysis of tumors which are difficult to dissociate to intact whole cells.
Gerlyng
P
P
Department of Tissue Culture, Norwegian Radium Hospital, Oslo.
Stokke
T
T
Huitfeldt
H S
HS
Stenersen
T
T
Danielsen
H E
HE
Grotmol
T
T
Seglen
P O
PO
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D006690:Bisbenzimidazole; D001973:Bromodeoxyuridine / Q000276:immunology; D002452:Cell Count; D002455:Cell Division; D002469:Cell Separation / Q000379:methods*; D004247:DNA / Q000032:analysis; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D007091:Image Processing, Computer-Assisted; D008099:Liver / Q000166:cytology*; D008115:Liver Regeneration; D008297:Male; D008856:Microscopy, Fluorescence; D011003:Ploidies; D051381:Rats; D011921:Rats, Inbred WKY; D013194:Staining and Labeling
D000911:Antibodies, Monoclonal; D004247:DNA; D001973:Bromodeoxyuridine; D006690:Bisbenzimidazole