Determination of luxabendazole in biological fluids by high-performance liquid chromatography.
Luxabendazole, a new benzimidazole, is a highly potent broad-spectrum anthelmintic. A high-performance liquid chromatographic method has been developed for its determination in serum and urine samples. In order to optimize the clean-up of samples we compared two procedures: C18 Sep-Pak cartridges and ultrafiltration through a cellulose membrane with a 30,000 relative molecular mass cut-off. In order to obtain the most suitable mobile phase, we studied the influence of pH and acetonitrile content on the capacity factor (k'). Chromatographic separation and quantification were performed on a reversed-phase column packed with 5-microns Nucleosil C18. The mobile phase was acetonitrile-0.05 M phosphate buffer (pH 7.0), (40:60, v/v). The column effluent was monitored by ultraviolet-visible spectrophotometry at 290 nm. The method shows good recovery, precision and accuracy. The lower limit of detection for luxabendazole is 15 ng/ml in serum samples and 25 ng/ml in urine samples.
Alvarez-Bujidos
M L
ML
Departamento de Fisiología, Farmacologia y Toxicología, Universidad de León, Spain.
Ortiz
A
A
Balaña
R
R
Cubría
J C
JC
Ordoñez
D
D
Negro
A
A
D000818:Animals; D000871:Anthelmintics / Q000097:blood / Q000378:metabolism* / Q000652:urine; D001562:Benzimidazoles / Q000097:blood / Q000378:metabolism* / Q000652:urine; D002219:Carbamates / Q000097:blood / Q000378:metabolism* / Q000652:urine; D002851:Chromatography, High Pressure Liquid; D011817:Rabbits; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet
D000871:Anthelmintics; D001562:Benzimidazoles; D002219:Carbamates; C058092:luxabendazole