Deletions in the short arm of chromosome 8 are present in up to 90% of human colorectal cancer cell lines.
Cytogenetic analyses of human colon cancer cells have revealed non-random deletions in chromosome arm 8p, among other chromosomal changes. By using 8p-specific DNA probes we could identify allelic loss in 87% of colon cancer cell lines. Corresponding analyses in direct preparations of colon tumor tissues revealed a minimal value of 40% of allelic loss but were obstructed in many instances by contaminating normal tissue. These findings add to the number of non-random genetic alterations occurring during colon carcinogenesis.
van der Bosch
K
K
Institut für Experimentelle Pathologie, Deutsches Krebsforschungszentrum, Heidelberg, Federal Republic of Germany.
Becker
I
I
Savelyeva
L
L
Brüderlein
S
S
Schlag
P
P
Schwab
M
M
D000483:Alleles; D002872:Chromosome Deletion; D002898:Chromosomes, Human, Pair 8 / Q000648:ultrastructure*; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology; D015342:DNA Probes; D004273:DNA, Neoplasm / Q000032:analysis; D005819:Genetic Markers; D006801:Humans; D014407:Tumor Cells, Cultured
D015342:DNA Probes; D004273:DNA, Neoplasm; D005819:Genetic Markers