Human neutrophil azurocidin synergizes with leukocyte elastase and cathepsin G in the killing of Capnocytophaga sputigena.
Azurocidin was purified in the presence of phenylmethylsulfonyl fluoride. Electrophoresis revealed at least seven species which exhibited N-terminal sequences consistent with azurocidin. Azurocidin exhibited no bactericidal activity against Capnocytophaga sputigena or other oral bacteria but synergized the bactericidal activity of enzymatically active elastase. Azurocidin also interacted synergistically with cathepsin G.
Miyasaki
K T
KT
Section of Oral Biology, UCLA School of Dentistry 90024-1668.
Bodeau
A L
AL
D000595:Amino Acid Sequence; D023181:Antimicrobial Cationic Peptides; D001770:Blood Bactericidal Activity; D001798:Blood Proteins / Q000008:administration & dosage*; D002206:Capnocytophaga / Q000276:immunology*; D002352:Carrier Proteins; D002402:Cathepsin D / Q000008:administration & dosage*; D003594:Cytoplasmic Granules / Q000201:enzymology; D006801:Humans; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D009504:Neutrophils / Q000201:enzymology*; D010196:Pancreatic Elastase / Q000008:administration & dosage*
C062991:AZU1 protein, human; D023181:Antimicrobial Cationic Peptides; D001798:Blood Proteins; D002352:Carrier Proteins; D010196:Pancreatic Elastase; D002402:Cathepsin D