Growth of a human yolk sac tumor cell line with yolk sac-derived functions in selenium-supplemented chemically defined synthetic medium.
A human yolk sac tumor cell line, TG1, which was established from a testicular yolk sac tumor, was found to replicate continuously in a chemically defined medium supplemented with Na2SeO3 (ISRPMI). TG1 produced several plasma proteins and growth factors: albumin, alpha-fetoprotein (AFP), ferritin, carcinoembryonic antigen, beta-2-microglobulin, polyamine, neuron specific enolase, tissue polypeptide antigen, transferrin (Tf), epidermal growth factor, and platelet derived growth factor. By analysis of lentil lectin (LcHA)-affinity electrophoresis, to examine the microheterogeneity of carbohydrate chains of synthetic glycoproteins, TG1 cells cultured with ISRPMI produced only LcHA reactive Tf and AFP based on core fucose attached to asparagine-linked N-acetylglucosamine residues instead of LcHA-nonreactive Tf and AFP produced by TG1 cells cultured with fetal bovine serum (FBS)-containing medium. alpha 1-6 Fucosyltransferase activity was significantly greater in the TG1 cells cultured with ISRPMI (39.9 +/- 1.5 pmol.h-1.mg-1 protein) than cultured with FBS-containing media (18.2 +/- 1.2 pmol.h-1.mg-1 protein). These results have indicated that the selective increase of alpha 1-6 fucosyltransferase occurred when the cells were cultured with the FBS-free synthetic media.
Ohkawa
K
K
Department of Biochemistry, Jikei University School of Medicine, Tokyo, Japan.
Hatano
T
T
Takizawa
N
N
Shinmoto
K
K
Yamada
K
K
Matsuda
M
M
Takada
K
K
Tsukada
Y
Y
D002272:Carcinoembryonic Antigen / Q000378:metabolism; D002455:Cell Division / Q000187:drug effects; D003470:Culture Media / Q000737:chemistry / Q000494:pharmacology*; D004815:Epidermal Growth Factor / Q000378:metabolism; D005647:Fucosyltransferases / Q000032:analysis / Q000378:metabolism; D016695:Glycosyltransferases / Q000378:metabolism / Q000502:physiology; D006801:Humans; D007150:Immunohistochemistry; D037102:Lectins; D008297:Male; D008649:Mesonephroma / Q000201:enzymology / Q000378:metabolism / Q000473:pathology*; D010751:Phosphopyruvate Hydratase / Q000378:metabolism; D010982:Platelet-Derived Growth Factor / Q000378:metabolism; D011073:Polyamines / Q000378:metabolism; D012643:Selenium / Q000032:analysis / Q000494:pharmacology*; D012710:Serum Albumin, Bovine / Q000032:analysis / Q000494:pharmacology; D013736:Testicular Neoplasms / Q000201:enzymology / Q000378:metabolism / Q000473:pathology*; D014168:Transferrin / Q000378:metabolism; D014407:Tumor Cells, Cultured / Q000201:enzymology / Q000378:metabolism / Q000473:pathology; D000509:alpha-Fetoproteins / Q000378:metabolism; D001613:beta 2-Microglobulin / Q000378:metabolism
D002272:Carcinoembryonic Antigen; D003470:Culture Media; D037102:Lectins; D010982:Platelet-Derived Growth Factor; D011073:Polyamines; D014168:Transferrin; D000509:alpha-Fetoproteins; D001613:beta 2-Microglobulin; D012710:Serum Albumin, Bovine; D004815:Epidermal Growth Factor; D016695:Glycosyltransferases; D005647:Fucosyltransferases; D010751:Phosphopyruvate Hydratase; D012643:Selenium