Bacteriophage lambda DNA fragments replicate in the Paramecium macronucleus: absence of active copy number control.
We show that bacteriophage lambda DNA fragments microinjected into the macronucleus of the ciliated protozoan Paramecium can replicate as unit-length linear molecules. These linear DNA molecules are substrates for the addition of Paramecium telomeres by an endogenous telomerase. The linear DNA pieces can exist at copy numbers much higher than that of typical endogenous macronuclear chromosomes. We show that the copy number of injected DNA many fissions after microinjection reflects that of the original input copy number, suggesting that active control of copy number does not occur. Instead, the results suggest that injected DNA is replicated once per cell division.
Kim
C S
CS
Department of Biology, Indiana University, Bloomington 47405.
Preer
J R
JR
Polisky
B
B
D000818:Animals; D010582:Bacteriophage lambda / Q000235:genetics*; D002467:Cell Nucleus / Q000378:metabolism*; D004261:DNA Replication; D004279:DNA, Viral / Q000096:biosynthesis*; D008845:Microinjections; D010247:Paramecium / Q000382:microbiology*; D014170:Transformation, Genetic
D004279:DNA, Viral