Conjugated 1 beta-hydroxycholic acid in the urine of newborns and pregnant women measured by radioimmunoassay using antisera raised against N-(1 beta-hydroxycholyl)-2-aminopropionic acid-bovine serum albumin conjugate.
Anti-tauro 1 beta-hydroxycholic acid antisera were prepared by immunizing rabbits with N-(1 beta,3 alpha, 7 alpha, 12 alpha-tetrahydroxy-5 beta-cholan-24-oyl)-2- aminopropionic acid-bovine serum albumin (BSA) conjugate. The antisera raised had high affinity (1.25-1.46 x 10(9) M-1) and specificity for conjugated 1 beta-hydroxycholic acid; cross-reactivity for glyco 1 beta-hydroxycholic acid was 100% and that for the glycine and taurine conjugates of other 1 beta-hydroxylated bile acids ranged from 11.30 to 0.23%. Urinary concentrations of conjugated 1 beta-hydroxycholic acid were determined by radioimmunoassay in newborns, 0-20 d after birth, in amounts ranging from 0.29 to 18.51 micrograms/ml and in women in late pregnancy (less than 1.13 micrograms/ml), as well as in normal women (less than 0.12 microgram/ml).
Ikegawa
S
S
Faculty of Pharmaceutical Sciences, Higashi-Nippon-Gakuen University, Hokkaido, Japan.
Murai
T
T
Yoshimura
T
T
Tohma
M
M
D000818:Animals; D001647:Bile Acids and Salts / Q000652:urine*; D002757:Cholanes / Q000652:urine*; D002793:Cholic Acids / Q000138:chemical synthesis* / Q000494:pharmacology; D003429:Cross Reactions; D005260:Female; D006801:Humans; D011247:Pregnancy; D011817:Rabbits; D011863:Radioimmunoassay; D012710:Serum Albumin, Bovine / Q000276:immunology
D001647:Bile Acids and Salts; D002757:Cholanes; D002793:Cholic Acids; C077359:N-(1,3,7,12-tetrahydroxycholan-24-oyl)-2-aminopropionic acid; D012710:Serum Albumin, Bovine; C035924:1,3,7,12-tetrahydroxycholanoic acid