title: Methods for the analysis of persistent chlorinated hydrocarbons in tissues.
abstract: Chlorinated hydrocarbons bioaccumulate in tissues and may have severe health consequences. These compounds occur individually, in small groups or as complex mixtures; examples of each category include aldrin, hexachlorocyclohexane and the polychlorinated biphenyls. Tissue extraction and purification schemes have been established, although new approaches such as supercritical fluid extraction are promising. Analyses often require the resolving power of capillary gas chromatography, in combination with the sensitivity and selectivity of electron-capture detection, electrolytic conductivity detection and mass spectrometry. Difficulties arise in quantitating chlorinated hydrocarbons in tissues, due to the number of components present and the fact that individual constituents may be reduced or enhanced in concentration in tissues, compared with the original formulation. Congener specific analysis and computer-assisted identification techniques have been applied to the problem.
authors.affiliation: Division of Chemistry and Toxicology, Virginia Institute of Marine Science, College of William and Mary, Gloucester Point 23062.
authors.forename: R C
authors.initials: RC
authors.lastname: Hale
authors.forename: J
authors.initials: J
authors.lastname: Greaves
mesh_terms: D002845:Chromatography / Q000379:methods; D004563:Electrochemistry; D006801:Humans; D006843:Hydrocarbons, Chlorinated / Q000032:analysis*; D013058:Mass Spectrometry; D013048:Specimen Handling
chemical_list: D006843:Hydrocarbons, Chlorinated