Retinal photoisomerase: role in invertebrate visual cells.
In invertebrate visual cells, the rhodopsin content is maintained at a high level by the fast process of photoregeneration during daylight. Rhodopsin is converted by photoabsorption to metarhodopsin, which is reconverted to rhodopsin by light. In addition, rhodopsin is regenerated by a slow process of renewal which takes days to complete and involves the biosynthesis of opsin. It is well known that rhodopsin can be formed from opsin only when 11-cis-retinal is present; this requires the existence of an isomerizing enzyme which is capable of transforming all-trans-retinal, released from the degradation of metarhodopsin, into the 11-cis-retinal isomer. In some invertebrate visual systems, experiments on rhodopsin regeneration have been interpreted by assuming that the isomerization reaction is a light-dependent process involving a retinal-protein complex. Two retinal photoisomerases which have been well characterized, i.e. bee photoisomerase and cephalopod retinochrome, are reviewed here. Their properties are compared in order to determine their physiological role, which is likely to be in the renewal of visual pigment rhodopsin. To conclude, a visual pigment cycle is proposed in which rhodopsin regeneration follows two light-dependent pathways. This greatly simplifies the rhodopsin regeneration scheme for invertebrate visual systems.
Pepe
I M
IM
Istituto di Cibernetica e Biofisica del C.N.R., Genova, Italy.
Cugnoli
C
C
D000818:Animals; D007448:Invertebrates; D007535:Isomerases / Q000378:metabolism*; D008027:Light; D012160:Retina / Q000201:enzymology / Q000502:physiology*; D012243:Rhodopsin / Q000378:metabolism; D014785:Vision, Ocular / Q000502:physiology*; D019745:cis-trans-Isomerases
C077124:retinal isomerase; D012243:Rhodopsin; D007535:Isomerases; D019745:cis-trans-Isomerases