CD20 and CD5 expression in B-chronic lymphocytic leukemia.
In order to quantitate a previously noted decrease in CD20 fluorescence intensity (FI) on B-CLL lymphocytes, binding capacities [BC x 10(3) +/- 1SD = number of antibodies bound per cell] were calculated. The mean (N = 5) BC x 10(3) +/- 1SD of CD20 reagents for normal B-PBL and B-CLL lymphocytes confirmed this observation. B-PBL and B-CLL were 56 +/- 11 and 61 +/- 14, and 19 +/- 15 and 18 +/- 16, respectively, for Leu 16 and B1. Although adequate compensation standards for the determination of CD5 and CD20 coexpression are not available, qualitatively, the density of CD5 on both normal B-PBL and B-CLL is less compared to the expression of CD5 by normal T cells. CD5 expression on B-CLL seems to be linked to the lower levels of CD20, whereas CD5 expression may appear to be absent on CLL lymphocytes expressing normal levels of CD20. Levels of CD20 in B-CLL suggest involvement of one or two genes (alleles) whose decreased expression may be linked to CD5 expression.
Marti
G E
GE
Laboratory of Cellular and Molecular Biology, Food and Drug Administration, National Institutes of Health, Bethesda, Maryland 20892.
Faguet
G
G
Bertin
P
P
Agee
J
J
Washington
G
G
Ruiz
S
S
Carter
P
P
Zenger
V
V
Vogt
R
R
Noguchi
P
P
D015703:Antigens, CD / Q000032:analysis*; D018941:Antigens, CD19; D018951:Antigens, CD20; D000944:Antigens, Differentiation, B-Lymphocyte / Q000032:analysis*; D016175:B-Lymphocyte Subsets / Q000276:immunology*; D001666:Binding Sites, Antibody; D018956:CD5 Antigens; D006801:Humans; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000276:immunology*; D012016:Reference Values
D015703:Antigens, CD; D018941:Antigens, CD19; D018951:Antigens, CD20; D000944:Antigens, Differentiation, B-Lymphocyte; D018956:CD5 Antigens