title: K+ channel involvement in induction of synaptic enhancement by mast cell degranulating (MCD) peptide.
abstract: A bee venom, mast cell degranulating peptide (MCD), which induces long-term potentiation (LTP) of synaptic transmission in hippocampal slices, was found to possess multiple functions. They include (1) binding and thereby inhibiting a voltage-dependent K(+)-channel in brain membranes, (2) incorporation in a lipid bilayer to form voltage-dependent and cation-selective channels by itself, and (3) activation of a pertussis toxin (Ptx)-sensitive GTP-binding proteins. In this study, we prepared several derivatives and analogues of MCD and investigated which function is more closely related to the induction of LTP. Another bee venom, apamin, formed ion channels in a lipid bilayer which were indistinguishable from those formed by MCD. D-MCD, an optical isomer of MCD, activated a Ptx-sensitive GTP-binding protein. However, these peptides did not induce LTP in the hippocampal slices. A snake venom, dendrotoxin-I (DTX-I), bound to the same K(+)-channels as MCD and did induce LTP. These results suggest that the most potent aspect of MCD involved in LTP inducibility is its interaction with the voltage-dependent K(+)-channel.
authors.affiliation: Institute for Protein Research, Osaka University, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Kondo
authors.forename: K
authors.initials: K
authors.lastname: Ikenaka
authors.forename: I
authors.initials: I
authors.lastname: Fujimoto
authors.forename: S
authors.initials: S
authors.lastname: Aimoto
authors.forename: H
authors.initials: H
authors.lastname: Kato
authors.forename: K
authors.initials: K
authors.lastname: Ito
authors.forename: T
authors.initials: T
authors.lastname: Taguchi
authors.forename: T
authors.initials: T
authors.lastname: Morita
authors.forename: M
authors.initials: M
authors.lastname: Kasai
authors.forename: K
authors.initials: K
authors.lastname: Mikoshiba
mesh_terms: D000818:Animals; D001514:Bee Venoms / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D004546:Elapid Venoms / Q000494:pharmacology; D006624:Hippocampus / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D066298:In Vitro Techniques; D007473:Ion Channels / Q000502:physiology; D008051:Lipid Bilayers; D008407:Mast Cells / Q000378:metabolism; D009474:Neurons / Q000187:drug effects / Q000502:physiology; D009498:Neurotoxins / Q000494:pharmacology; D009994:Osmolar Concentration; D010455:Peptides / Q000494:pharmacology*; D015221:Potassium Channels / Q000502:physiology*; D051381:Rats; D011919:Rats, Inbred Strains; D013569:Synapses / Q000187:drug effects*
chemical_list: D001514:Bee Venoms; D004546:Elapid Venoms; D007473:Ion Channels; D008051:Lipid Bilayers; D009498:Neurotoxins; D010455:Peptides; D015221:Potassium Channels; C008331:mast cell degranulating peptide; C020273:dendrotoxin; D002118:Calcium