Use of Brefeldin A to localize block in intracellular transport of vesicular stomatitis virus G protein on interferon-treated cells.
Brefeldin A (BFA) induced a rapid redistribution of vesicular stomatitis virus G protein (VSV-G) to the endoplasmatic reticulum (ER) in interferon (IFN)-pretreated cells. This result is consistent with accumulation of VSV-G in the trans-Golgi (GC) complex in cells pretreated with IFN and implies that IFN does not interfere with the ability of BFA to induce redistribution of proteins from GC to ER.
Polakova
K
K
Cancer Research Institute, Slovak Academy of Sciences, Bratislava, Czechoslovakia.
Russ
G
G
D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D020126:Brefeldin A; D003517:Cyclopentanes / Q000494:pharmacology*; D005455:Fluorescent Antibody Technique; D006056:Golgi Apparatus / Q000378:metabolism*; D007372:Interferons / Q000494:pharmacology*; D007739:L Cells; D008562:Membrane Glycoproteins; D051379:Mice; D014721:Vesicular stomatitis Indiana virus / Q000187:drug effects / Q000378:metabolism*; D014759:Viral Envelope Proteins / Q000187:drug effects / Q000302:isolation & purification / Q000378:metabolism*; D014777:Virus Diseases / Q000378:metabolism
D003517:Cyclopentanes; C028391:G protein, vesicular stomatitis virus; D008562:Membrane Glycoproteins; D014759:Viral Envelope Proteins; D020126:Brefeldin A; D007372:Interferons