Role of vitamin A in the haematotoxicity of hexachlorocyclohexane (HCH) in the rat.
The haematotoxicity of technical hexachlorocyclohexane (HCH) (1000 ppm) was investigated in male albino rats fed with diet free of vitamin A or containing vitamin A at 2000 or 10(5) I.U./kg. Assessment of HCH-induced haematotoxicity at the end of the 7 weeks feeding period was done on the basis of haemoglobin content, total count of red blood cells and white blood cells and the differential counts of the white blood cells as well as by parameters such as packed cell volume, mean corpuscular volume, mean corpuscular haemoglobin, mean corpuscular haemoglobin content, prothrombin time and clotting time. In the rats fed with vitamin A-free diet containing HCH, significant reductions were noticed in the total white blood cells count, clotting time and prothrombin time indicating severe haematotoxicity. Differential count of the white blood cells of these rats revealed a non-significant reduction in the lymphocyte count. The only indication of haematotoxicity caused by hexachlorocyclohexane in the vitamin A supplemented rats was a slight but statistically significant reduction of the total count of white blood cells. These results demonstrate that the haematotoxicity of hexachlorocyclohexane in the rats is enhanced by vitamin A-deficiency and its supplementation particularly in excess but not at hypervitaminotic level is protective against the toxicity.
Joseph
P
P
Central Food Technological Research Institute, Mysore, India.
Viswanatha
S
S
Krishnakumari
M K
MK
D000818:Animals; D001773:Blood Cells / Q000187:drug effects*; D004347:Drug Interactions; D004906:Erythrocyte Count / Q000187:drug effects / Q000662:veterinary; D006454:Hemoglobins / Q000032:analysis; D001556:Hexachlorocyclohexane / Q000633:toxicity*; D007958:Leukocyte Count / Q000187:drug effects / Q000662:veterinary; D008297:Male; D011897:Random Allocation; D051381:Rats; D011919:Rats, Inbred Strains; D014801:Vitamin A / Q000494:pharmacology*
D006454:Hemoglobins; D014801:Vitamin A; D001556:Hexachlorocyclohexane