High-temperature short-time heat inactivation of HIV and other viruses in human blood plasma.
An ultra-short-time heating system was used to process blood plasma spiked with various viruses (HIV, vesicular stomatitis virus, encephalomyocarditis virus). Virus reduction and recovery of plasma proteins were measured at various temperatures from 65 to 85 degrees C. Processing at 77 degrees C and 0.006 s resulted in a high level of virus kill, including greater than or equal to 4.4 log10 HIV, while maintaining protein structure and activity essentially intact.
Charm
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Charm Bioengineering, Inc., Malden, MA 02148.
Landau
S
S
Williams
B
B
Horowitz
B
B
Prince
A M
AM
Pascual
D
D
D001769:Blood / Q000382:microbiology*; D001779:Blood Coagulation Factors / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D015488:HIV Antigens / Q000032:analysis; D015658:HIV Infections / Q000097:blood / Q000635:transmission; D006358:Hot Temperature; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D013997:Time Factors; D014773:Virology / Q000379:methods*; D014777:Virus Diseases / Q000097:blood / Q000635:transmission; D005719:gamma-Globulins / Q000032:analysis
D001779:Blood Coagulation Factors; D015488:HIV Antigens; D007074:Immunoglobulin G; D005719:gamma-Globulins