Simultaneous and iterative weighted regression analysis of toxicity tests using a microplate reader.
A system is described for determination of LC50 or IC50 by an iterative process based on data obtained from a plate reader using a marine unicellular alga as a target species. The esterase activity of Tetraselmis suesica on fluorescein diacetate as a substrate was measured using a fluorescence titerplate. Simultaneous analysis of results was performed using an iterative process adopting the sigmoid function Y = y/1 (dose of toxicant/IC50)slope for dose-response relationships. IC50 (+/- SEM) was estimated (P less than 0.05). An application with phosalone as a toxicant is presented.
Galgani
F
F
IFREMER, Nantes, France.
Cadiou
Y
Y
Gilbert
F
F
D002800:Cholinesterase Inhibitors / Q000633:toxicity; D003201:Computers; D004950:Esterases / Q000378:metabolism; D056890:Eukaryota; D007928:Lethal Dose 50; D009946:Organothiophosphorus Compounds / Q000633:toxicity; D012044:Regression Analysis; D014116:Toxicology / Q000379:methods*; D014874:Water Pollutants, Chemical / Q000633:toxicity
D002800:Cholinesterase Inhibitors; D009946:Organothiophosphorus Compounds; D014874:Water Pollutants, Chemical; C003117:phosalone; D004950:Esterases