title: Lack of increased cardiac output during hemoglobin hemodilution can be reversed with sodium nitroprusside.
abstract: To investigate a possible connection between EDRF or nitric oxide (NO) and the unchanged cardiac output (CO) during hemoglobin-hemodilution we infused nitroprusside (NP) in eight Hb-diluted dogs (Hct approximately 20%). Normal hypotensive doses of NP were not effective and supranormal doses (133.0 micrograms/kg/min) were needed to induce even a modest decrease in mean AoP (approximately 25 mmHg). With these NP doses, cardiac output increased 177%, diastolic AoP (afterload) decreased 30%, while systolic AoP and LVEDP (preload) were unchanged. Heart rate, LV contractility (pressure-volume function) and blood volume were not changed throughout the study. Normally, NP alone decreases both preload and afterload resulting in unchanged CO. In the Hb + NP dogs, CO increased because only afterload decreased suggesting a selective effect of Hb on venous and arterial smooth muscle relaxation. In hemodilution with nonhemoglobin colloids, CO increases primarily because the diluted blood offers less viscous resistance to ventricular ejection. It appears that in order for cardiac output to increases in the presence of Hb, some decrease in arteriolar resistance is needed, presumably to unmask the effects of reduced viscosity. These results suggest the unchanged CO during Hb-dilution is related to a selective effect of Hb on venous and arteriolar nitric oxide (EDRF) function.
authors.affiliation: Department of Anesthesiology, Loyola University Medical Center, Maywood, IL.
authors.forename: M W
authors.initials: MW
authors.lastname: Rooney
authors.forename: L J
authors.initials: LJ
authors.lastname: Hirsch
authors.forename: M K
authors.initials: MK
authors.lastname: Aasen
authors.forename: S A
authors.initials: SA
authors.lastname: Goldberg
authors.forename: M
authors.initials: M
authors.lastname: Mathru
mesh_terms: D000818:Animals; D001802:Blood Substitutes / Q000494:pharmacology*; D001809:Blood Viscosity; D002302:Cardiac Output / Q000187:drug effects* / Q000502:physiology; D004285:Dogs; D006438:Hemodilution; D006454:Hemoglobins / Q000494:pharmacology*; D009569:Nitric Oxide / Q000378:metabolism / Q000502:physiology; D009599:Nitroprusside / Q000008:administration & dosage / Q000494:pharmacology*; D014655:Vascular Resistance
chemical_list: D001802:Blood Substitutes; D006454:Hemoglobins; D009599:Nitroprusside; D009569:Nitric Oxide