title: Nonselective cation channels in intact human T lymphocytes.
abstract: Nonselective cation channels were found in single channel recordings from cell-attached patches on human T lymphocytes. These channels were active under conditions that should lead to cell swelling (hypotonic bath solutions with NaCl or KCl); however, a definite dependence of activity on cell swelling has not been proven. Under these conditions similar channels were found in 20 of 23 patches from 11 different blood donors. The current-voltage relation was approximately linear for outward current (11-14 pS) and inwardly rectifying (to 23 pS) when the intact cells were depolarized with high KCl in the bath. The voltage dependence of channel activity is consistent with closing at hyperpolarized membrane potentials (Vm less than or equal to -50 mV) and block of open channels at strongly depolarized membrane potentials (Vm greater than 0 mV). Reversal potentials under all ionic gradients tested are consistent with a channel that is poorly selective between Na+ and K+ ions. Active channels in cell-attached patches were rapidly blocked by bath addition of the membrane-permeant inhibitor quinine. Channels that were active in cell-attached became quiescent after patch excision; however, two patches remained active long enough to obtain current-voltage relations. These were linear with a slope conductance for outward current of 8-11 pS. Because of the clustering of single-channel openings, detailed voltage dependence of kinetics and probability of opening were not studied.
authors.affiliation: Department of Physiology, University of Toronto, Ont., Canada.
authors.forename: L C
authors.initials: LC
authors.lastname: Schlichter
mesh_terms: D002412:Cations / Q000378:metabolism*; D004594:Electrophysiology; D006801:Humans; D007038:Hypotonic Solutions; D066298:In Vitro Techniques; D007473:Ion Channels / Q000187:drug effects / Q000378:metabolism*; D008564:Membrane Potentials / Q000187:drug effects; D009994:Osmolar Concentration; D011189:Potassium Chloride / Q000494:pharmacology; D011803:Quinine / Q000494:pharmacology; D012965:Sodium Chloride / Q000494:pharmacology; D013601:T-Lymphocytes / Q000187:drug effects / Q000378:metabolism*
chemical_list: D002412:Cations; D007038:Hypotonic Solutions; D007473:Ion Channels; D012965:Sodium Chloride; D011189:Potassium Chloride; D011803:Quinine