Nuclear area measurement on viable cells, using confocal microscopy.
We describe a rapid procedure for the accurate measurements of nuclear areas on unperturbed living cells as used in radiobiological experiments, using the confocal laser scanning microscope. The microdosimetric interpretation of radiobiological data requires precise information on the nuclear area of cells as irradiated with high-LET radiation.
Townsend
K M
KM
Medical Research Council, Didcot, Oxon, UK.
Marsden
S J
SJ
D000818:Animals; D002460:Cell Line; D002467:Cell Nucleus / Q000648:ultrastructure*; D006224:Cricetinae; D003412:Cricetulus; D006801:Humans; D066298:In Vitro Techniques; D007834:Lasers; D051379:Mice; D008853:Microscopy / Q000295:instrumentation / Q000379:methods; D008928:Mitochondria / Q000648:ultrastructure; D020112:Rhodamine 123; D012235:Rhodamines; D013194:Staining and Labeling
D012235:Rhodamines; D020112:Rhodamine 123