[The preparation and identification of anti-idiotypic antibody against Plasmodium vivax at erythrocytic stages].
Through hypotonic dialyzing and gel filtration on Sephadex G200, 6H7G11 McAb-IgM (Mouse IgM) was successfully purified. Anti-6H7G11Id (Ab2) was induced in rabbit. This Ab2 was identified by agar diffusion, ELISA and competitive assays. The results showed that the Ab2 was able to combine respective Ab1 (6H7G11McAb), and the binding between Ab2 and Ab1 could be inhibited by Plasmodium vivax (P.v) antigen. Meantime, by using Ab2 thus prepared to immunize BALB/c mice, the anti-6H7G11Id (Ab3 or Ab1') was obtained with specificity of Ab1. It was revealed that the Ab2 thus prepared could mimic the antigenic determinant of P.v, and it belongs to the cross-reacted idiotype.
Bai
H
H
Hu
A
A
Zhu
S
S
Wang
X
X
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000096:biosynthesis* / Q000302:isolation & purification; D000911:Antibodies, Monoclonal; D000913:Antibodies, Protozoan / Q000096:biosynthesis* / Q000302:isolation & purification; D000939:Epitopes; D004912:Erythrocytes / Q000469:parasitology; D005260:Female; D007075:Immunoglobulin M; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010966:Plasmodium vivax / Q000276:immunology*; D011817:Rabbits
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D000913:Antibodies, Protozoan; D000939:Epitopes; D007075:Immunoglobulin M