Discrimination between two types of P2 purinoceptors by suramin in rat hepatocytes.
Suramin, currently reported as a P2 purinoceptor antagonist, competitively inhibited P2 purinoceptor agonist-induced phospholipase C (PLC) stimulation, but not P2 purinoceptor agonist-induced adenylate cyclase (AC) inhibition in rat hepatocytes. Suramin did not inhibit vasopressin-induced PLC activity. We conclude that there are two types of P2 purinoceptors; one is suramin-sensitive and coupled to PLC in a stimulatory manner, and the other is suramin-insensitive and coupled to AC in an inhibitory manner.
Tomura
H
H
Department of Physical Biochemistry, Institute of Endocrinology, Maebashi, Japan.
Okajima
F
F
Kondo
Y
Y
D000067956:Adenylyl Cyclase Inhibitors; D000818:Animals; D001665:Binding Sites; D008099:Liver / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D058914:Purinergic Antagonists; D051381:Rats; D017208:Rats, Wistar; D011983:Receptors, Purinergic / Q000187:drug effects*; D013498:Suramin / Q000494:pharmacology*; D010738:Type C Phospholipases / Q000378:metabolism; D014667:Vasopressins / Q000494:pharmacology
D000067956:Adenylyl Cyclase Inhibitors; D058914:Purinergic Antagonists; D011983:Receptors, Purinergic; D014667:Vasopressins; D013498:Suramin; D010738:Type C Phospholipases