[Preparation of a new tissue-based biosensor].
A new adenosine electrode using rabbit thymus tissue as catalyst is described. The tissue membrane was immobilized at the surface of an ammonia gassensing electrode for determination of adenosine in body fluid or tissue samples. This tissue-based biosensor showed excellent selectivity, and sensitivity, with a response slope of 51.2mV/decade, linear range of 3.16 x 10(-5)-5.62 x 10(-3) mol/L, coefficients of variation ranging from 1% to 5.72% (n = 7, m = 5), recoveries of adenosine in rabbit blood from 94.9% to 108.4% (n = 5, m = 5), and response time of 7 min. The life-time of the electrode was 25 days and 14 analogous compounds showed no significant interference.
Gao
X
X
Li
Y
Y
Yin
G
G
D000241:Adenosine / Q000032:analysis*; D000641:Ammonia / Q000032:analysis; D000818:Animals; D015374:Biosensing Techniques; D011817:Rabbits; D013950:Thymus Gland
D000641:Ammonia; D000241:Adenosine