Sequence and expression of human protein kinase C-epsilon.
Two human homologues of protein kinase C-epsilon (E1 and E2) were isolated from two distinct cDNA libraries. Sequence comparisons to PKC-epsilon cDNAs from several species indicated that each of these human epsilon clones contained cloning artifacts. Thus, a composite PKC-epsilon (E3) clone was derived from clones E1 and E2. Human PKC-epsilon (E3) has an overall sequence identity of 90-92% at the nucleotide level compared to the previously characterized mouse, rat and rabbit clones. At the amino acid level, the deduced human epsilon sequence shows a 98-99% identity with the mouse, rat and rabbit sequences. Expression of the human PKC-epsilon clone in Sf9 cells confirmed that the recombinant protein displayed protein kinase C activity and phorbol ester binding activity. The recombinant protein was also recognized by two distinct epsilon-specific polyclonal antibodies.
Basta
P
P
Sphinx Pharmaceuticals Corporation, Molecular Biology Section, Durham, NC 27717.
Strickland
M B
MB
Holmes
W
W
Loomis
C R
CR
Ballas
L M
LM
Burns
D J
DJ
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA; D015723:Gene Library; D006657:Histones / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D004676:Myelin Basic Protein / Q000378:metabolism; D010704:Phorbols / Q000378:metabolism; D011493:Protein Kinase C / Q000235:genetics* / Q000378:metabolism; D051744:Protein Kinase C-epsilon; D013379:Substrate Specificity
D006657:Histones; D004676:Myelin Basic Protein; D010704:Phorbols; D004247:DNA; C497255:PRKCE protein, human; D011493:Protein Kinase C; D051744:Protein Kinase C-epsilon