title: Preparation and characterization of an antibody specific to the rat dopamine D2 receptor.
abstract: Antibodies specific to the dopamine D2 receptor have been raised in rabbits using synthetic peptides. The resulting antiserum was sensitive to picogram quantities of peptide as measured by enzyme-linked immunoassay and was shown to have a 33% cross-reactivity with partially purified D2 receptor protein. No detectable cross-reactivity with similarly prepared fungal membranes was observed. D2 receptor preparations from normal rat pituitary cells were used in Western blot analysis. Bands of M(r) = 95,000 and 34,000 were detected in these preparations with a third faint band at 120,000. These correspond to the pituitary D2 receptor.
authors.affiliation: Unit of Metabolic Medicine, St Mary's Hospital Medical School, Imperial College of Science, Technology and Medicine, Paddington, London, U.K.
authors.forename: J M
authors.initials: JM
authors.lastname: Johnston
authors.forename: D F
authors.initials: DF
authors.lastname: Wood
authors.forename: J D
authors.initials: JD
authors.lastname: James
authors.forename: D G
authors.initials: DG
authors.lastname: Johnston
mesh_terms: D000818:Animals; D000906:Antibodies / Q000737:chemistry*; D000918:Antibody Specificity; D015153:Blotting, Western; D002462:Cell Membrane / Q000378:metabolism; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D010902:Pituitary Gland / Q000378:metabolism; D011817:Rabbits; D051381:Rats; D017448:Receptors, Dopamine D2 / Q000276:immunology* / Q000378:metabolism; D012232:Rhizoctonia
chemical_list: D000906:Antibodies; D017448:Receptors, Dopamine D2