Cloning and sequencing of a rat type II activin receptor.
A full-length cDNA for a rat type II activin receptor was cloned by hybridization from a rat ovary cDNA library. The deduced amino acid sequence (513 residues) containing a single membrane-spanning domain and an intracellular kinase domain with predicted serine/threonine specificity. The amino acid sequence is 99.8% and 99.4% identical in the coding region with the previously cloned mouse and human type II activin receptor, and only 66.7% identical in the coding region with the previously cloned rat type IIB activin receptor. We examined the effect of PMSG-hCG on the mRNA level of type II activin receptor in immature rat ovaries. Northern blot analysis of ovarian RNA revealed two mRNAs (3.0 kb and 6.0 kb).
Shinozaki
H
H
Department of Obstetrics and Gynecology, Gunma University School of Medicine, Japan.
Ito
I
I
Hasegawa
Y
Y
Nakamura
K
K
Igarashi
S
S
Nakamura
M
M
Miyamoto
K
K
Eto
Y
Y
Ibuki
Y
Y
Minegishi
T
T
D029404:Activin Receptors; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D006064:Gonadotropins, Equine / Q000494:pharmacology; D006801:Humans; D008969:Molecular Sequence Data; D010053:Ovary / Q000187:drug effects / Q000502:physiology; D011061:Poly A / Q000235:genetics; D017346:Protein Serine-Threonine Kinases / Q000235:genetics*; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012333:RNA, Messenger; D051381:Rats; D017208:Rats, Wistar; D011956:Receptors, Cell Surface / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
D006063:Chorionic Gonadotropin; D006064:Gonadotropins, Equine; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D011061:Poly A; D012313:RNA; D004247:DNA; D017346:Protein Serine-Threonine Kinases; D029404:Activin Receptors