Identification of an ion channel-forming motif in the primary structure of tetanus and botulinum neurotoxins.
Synthetic peptides with amino acid sequences corresponding to predicted transmembrane segments of tetanus toxin were used as probes to identify a channel-forming motif. A peptide denoted TeTx II, with sequence GVVLLLEYIPEITLPVIAALSIA, forms cation-selective channels when reconstituted in planar lipid bilayers. The single channel conductance in 0.5 M NaCl or KCl is 28 +/- 3 and 24 +/- 2 pS, respectively. In contrast, a peptide with sequence NFIGALETTGVVLLLEYIPEIT, denoted as TeTx I, or a peptide with the same amino acid composition as TeTx II but with a randomized sequence, do not form channels. Conformational energy calculations show that a bundle of four amphipathic alpha-helices is a plausible structural motif underlying observable pore properties. The identified functional module may account for the channel-forming activity of both tetanus toxin and the homologous botulinum toxin A.
Montal
M S
MS
Department of Biology, University of California San Diego, La Jolla 92093-0319.
Blewitt
R
R
Tomich
J M
JM
Montal
M
M
D000595:Amino Acid Sequence; D001905:Botulinum Toxins / Q000737:chemistry*; D007473:Ion Channels / Q000737:chemistry*; D008051:Lipid Bilayers; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000737:chemistry; D010713:Phosphatidylcholines / Q000737:chemistry; D011487:Protein Conformation; D016415:Sequence Alignment; D013744:Tetanus Toxin / Q000737:chemistry*
D007473:Ion Channels; D008051:Lipid Bilayers; D010446:Peptide Fragments; D010713:Phosphatidylcholines; D013744:Tetanus Toxin; D001905:Botulinum Toxins