title: Insulin, at physiological concentrations, enhances the polymorphonuclear leukocyte chemotactic properties.
abstract: We investigated the influence of insulin on human polymorphonuclear leukocyte (PMN) chemotaxis induced by mediators in a microchamber assay. Insulin increased, with a dose-response relationship, chemotaxis induced by formyl-methionyl-leucyl-phenylalanine, calcium ionophore and phorbol-miristyl acetate (p = 0.0057, p = 0.0001 and p = 0.0215, respectively). The hormone effect was present also at the physiological concentration of 40 microU/ml. Our data show that insulin affects PMN activity in normal subjects and therefore support the hypothesis that insulin deficiency may be responsible for the impaired PMN function observed in diabetic patients in poor metabolic control.
authors.affiliation: Department of Clinical and Biological Sciences, University of Torino, San Luigi Gonzaga Hospital, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Cavalot
authors.forename: G
authors.initials: G
authors.lastname: Anfossi
authors.forename: I
authors.initials: I
authors.lastname: Russo
authors.forename: E
authors.initials: E
authors.lastname: Mularoni
authors.forename: P
authors.initials: P
authors.lastname: Massucco
authors.forename: S
authors.initials: S
authors.lastname: Burzacca
authors.forename: L
authors.initials: L
authors.lastname: Mattiello
authors.forename: M
authors.initials: M
authors.lastname: Trovati
mesh_terms: D000328:Adult; D002634:Chemotaxis, Leukocyte / Q000187:drug effects*; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D007328:Insulin / Q000494:pharmacology*; D007476:Ionophores / Q000494:pharmacology; D008297:Male; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects; D013268:Stimulation, Chemical; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
chemical_list: D007328:Insulin; D007476:Ionophores; D009240:N-Formylmethionine Leucyl-Phenylalanine; D013755:Tetradecanoylphorbol Acetate