Distinct breakpoints in band 11q23 of the t(4;11) and t(11;14) associated with leukocyte malignancy.
Several non-random translocation breakpoints associated with leukemia or lymphoma have been shown to occur in chromosome band 11q23 between the genes CD3G and PBGD, a distance of approximately 750 kb. A combination of yeast artificial chromosome (YAC) cloning, in situ hybridization, and pulsed field gel electrophoresis (PFGE) experiments has further refined the interval containing one of these breakpoints, t(4;11)(q21;q23), to within 200 kb of CD3G. We have extended the PFGE analysis to show that the t(4;11) breakpoint lies in a region of approximately 100 kb, situated 100 kb distal to CD3G. Furthermore, we show that a second 11q23 breakpoint, t(11;14)(q23;q32), which was also previously mapped between CD3G and PBGD, is distinct from that of the t(4;11) chromosome. The 11q23 sequences that are involved at the t(11;14) breakpoint are not present in a YAC containing the t(4;11) breakpoint. The t(11;14) breakpoint has been localized on the PFGE map of the CD3G-PBGD interval and is at least 110 kb distal to the t(4;11) breakpoint, thus demonstrating heterogeneity among 11q23 breakpoints.
Radice
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P
Department of Pathology, University of Cambridge, United Kingdom.
Tunnacliffe
A
A
D001483:Base Sequence; D002880:Chromosomes, Human, Pair 11 / Q000648:ultrastructure*; D002894:Chromosomes, Human, Pair 4 / Q000648:ultrastructure*; D005819:Genetic Markers; D006801:Humans; D007938:Leukemia / Q000235:genetics*; D008223:Lymphoma / Q000235:genetics*; D008969:Molecular Sequence Data; D011519:Proto-Oncogenes; D013601:T-Lymphocytes / Q000648:ultrastructure; D014178:Translocation, Genetic; D014407:Tumor Cells, Cultured
D005819:Genetic Markers