Determination of penicillin G in bovine plasma by high-performance liquid chromatography after pre-column derivatization.
A simple, selective, and sensitive liquid chromatographic method with ultraviolet detection was developed for the analysis of penicillin G in bovine plasma. The assay utilizes a simple extraction of penicillin G from plasma (with a known amount of penicillin V added as internal standard) with water, dilute sulphuric acid and sodium tungstate solutions, followed by concentration on a conditioned C18 solid-phase extraction column. After elution with 500 microliters of elution solution, the penicillins are derivatized with 500 microliters of 1,2,4-triazole-mercuric chloride solution at 65 degrees C for 30 min. The penicillin-mercury mercaptide complexes are separated by reversed-phase liquid chromatography on a C18 column. The method, which has a detection limit of 5 ng/ml (ppb) in bovine plasma, was used to quantitatively measure the concentrations of penicillin G in plasma of steers at a series of intervals after the intramuscular administration of a commercial formulation of procaine penicillin G.
Boison
J O
JO
Health of Animals Laboratory, Agriculture Canada, Saskatoon, Saskatchewan.
Korsrud
G O
GO
MacNeil
J D
JD
Keng
L
L
Papich
M
M
D000818:Animals; D002417:Cattle / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007202:Indicators and Reagents; D007273:Injections, Intramuscular; D008297:Male; D010400:Penicillin G / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics; D010404:Penicillin V / Q000097:blood
D007202:Indicators and Reagents; D010400:Penicillin G; D010404:Penicillin V