Cross-reacting and heterospecific monoclonal antibodies produced against arabis mosaic nepovirus.
Monoclonal antibodies (MAbs) were produced against arabis mosaic nepovirus (AMV). A hybridoma screening procedure was applied which involved the testing of culture supernatants, before the hybridomas were cloned to single cell lines, for their reaction with eight nepoviruses [AMV, cherry leafroll virus (CLRV), grapevine fanleaf virus (GFLV), peach rosette mosaic virus, raspberry ringspot virus (RRSV), tobacco ringspot virus, tomato black ring virus (TBRV) and tomato ringspot virus]. In addition to AMV-specific MAbs, this screening technique has allowed the selection of two cross-reacting MAbs: one reacting with AMV and GFLV, and one reacting with AMV and RRSV. This is the first report of MAbs cross-reacting with these nepoviruses. In addition, five heterospecific MAbs (HS-MAbs) could be selected: two reacting with RRSV, two with CLRV and one with TBRV. The usefulness of the screening technique that was applied for the selection of cross-reacting MAbs and HS-MAbs, and the potential use of such antibodies are discussed.
Frison
E A
EA
Plant Pathology Research Programme, International Board for Plant Genetic Resources, Rome, Italy.
Stace-Smith
R
R
D000911:Antibodies, Monoclonal / Q000276:immunology*; D002999:Clone Cells; D003429:Cross Reactions; D006825:Hybridomas; D009029:Mosaic Viruses / Q000145:classification / Q000276:immunology* / Q000302:isolation & purification; D010935:Plant Diseases / Q000382:microbiology; D011790:Quarantine
D000911:Antibodies, Monoclonal