Identification and tissue distribution of rabbit leucocyte antigens recognized by monoclonal antibodies.
Three monoclonal antibodies which recognize rabbit leucocytes have been characterized by immunofluorescence staining of a variety of cell populations and also by immunochemical techniques. The evidence obtained suggests that these antibodies recognize the rabbit equivalents of the CD58/LFA-3 (VC21), CD43/leukosialin (L11/135) and CD9 (MM2/57) antigens. A fourth antibody, RPN3/57, recognizes an antigen expressed strongly on T cells, thymocytes and neutrophils and at lower levels on platelets. It has not, however been possible to characterize the antigen recognized by RPN3/57 in molecular terms. Both L11/135 and RPN3/57 are useful reagents for the detection of T cells both by flow cytometry and by immunohistochemistry.
Wilkinson
J M
JM
Department of Biochemistry and Cell Biology, Hunterian Institute, Royal College of Surgeons, London, U.K.
Galea-Lauri
J
J
Sellars
R A
RA
Boniface
C
C
D000818:Animals; D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation / Q000032:analysis* / Q000737:chemistry; D001792:Blood Platelets / Q000276:immunology; D004912:Erythrocytes / Q000276:immunology; D007962:Leukocytes / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008970:Molecular Weight; D009000:Monocytes / Q000276:immunology; D009504:Neutrophils / Q000276:immunology; D011817:Rabbits / Q000276:immunology*; D013045:Species Specificity; D013601:T-Lymphocytes / Q000276:immunology
D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation