Use of chemical cleavage to release active HIV-1 proteinase from a fusion protein produced in the form of insoluble inclusion bodies.
Gaskin
D J
DJ
Department of Crystallography, Birkbeck College, London.
Wilderspin
A F
AF
D001224:Aspartic Acid; D003001:Cloning, Molecular / Q000379:methods; D003062:Codon / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D005813:Genes, Synthetic; D016333:HIV Protease / Q000235:genetics / Q000302:isolation & purification*; D015497:HIV-1 / Q000201:enzymology* / Q000235:genetics; D006868:Hydrolysis; D007202:Indicators and Reagents; D016297:Mutagenesis, Site-Directed; D011993:Recombinant Fusion Proteins / Q000737:chemistry*; D011994:Recombinant Proteins / Q000302:isolation & purification
D003062:Codon; D007202:Indicators and Reagents; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins; D001224:Aspartic Acid; D016333:HIV Protease