Preferential chromosome loss in human papilloma virus DNA-immortalized mammary epithelial cells.
Human papilloma virus (HPV) DNA-immortalized human mammary epithelial cells may provide a model system for studying the molecular basis of immortalization and its role in breast neoplasia. Cytogenetic analyses were performed on clones derived from HPV 16- and HPV 18-immortalized human mammary epithelial cells. The majority of the clones contained near-diploid karyotypes. The single most frequent whole-chromosome loss was that of chromosome 19. Regions that showed preference for deletion and/or translocation included 2pter, 11qter, and 15pter. Evidence of chromosome 19 loss was confirmed by polymerase chain reaction (PCR)-generated, chromosome 19-specific, dinucleotide microsatellite repeat polymorphism analysis.
Swisshelm
K
K
Division of Cancer Genetics, Dana-Farber Cancer Institute, Boston, Massachusetts 02115.
Leonard
M
M
Sager
R
R
D001940:Breast / Q000382:microbiology / Q000648:ultrastructure*; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral / Q000235:genetics*; D002869:Chromosome Aberrations; D002872:Chromosome Deletion; D002888:Chromosomes, Human, Pair 19; D004279:DNA, Viral / Q000235:genetics; D005260:Female; D006801:Humans; D007621:Karyotyping; D027383:Papillomaviridae / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics; D014162:Transfection
D004279:DNA, Viral