Endotoxin of Pseudomonas pseudomallei detected by the body weight-decreasing reaction in mice and comparison of it with those of P. cepacia and P. aeruginosa.
The heat-treated whole cells, culture supernatants, and extracted endotoxin preparations of Pseudomonas pseudomallei were examined for endotoxin by the mouse body weight-decreasing (BWD) test. The experiments were conducted also with those of P. cepacia and P aeruginosa. Endotoxin was detected in all the samples of P. pseudomallei. Endotoxin of P. cepacia was detected in whole cells, but not in culture supernatant. The BWD activity of P. aeruginosa was 30 times as high as that of P. pseudomallei. This result was confirmed by the experiments with endotoxin preparations. In the limulus amebocyte lysate gelation (LAL) test, however, the endotoxin preparations of the two species showed the same level of activity.
Iwasa
S
S
National Institute of Health, Department of Medical Sciences, Ministry of Public Health, Nonthaburi, Thailand.
Petkanchanopong
W
W
Naigowit
P
P
Kondo
E
E
Kanai
K
K
D000818:Animals; D016956:Burkholderia cepacia; D016957:Burkholderia pseudomallei; D004731:Endotoxins / Q000032:analysis*; D006358:Hot Temperature; D008033:Limulus Test; D008070:Lipopolysaccharides / Q000032:analysis*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D010567:Pertussis Vaccine / Q000494:pharmacology; D011550:Pseudomonas aeruginosa; D015431:Weight Loss / Q000187:drug effects
D004731:Endotoxins; D008070:Lipopolysaccharides; D010567:Pertussis Vaccine