Northern and Southern blot analysis of human RNA and DNA in autopsy material.
Fresh biopsy material for molecular biological investigations is not obtainable from all relevant normal human tissues. We studied the feasibility of using RNA and DNA from autopsies for Northern and Southern blot analysis. Tissue samples from seven organs were obtained from 10 autopsies performed 21-118 h postmortem. Extracted RNA and DNA were examined by Northern and Southern blot analysis using oligo-labelled human DNA probes recognizing gene transcripts of 2-5 kb. The results indicated that, in general, Northern blot analysis was feasible with the applied probes when the tissue was obtained less than two days postmortem. Histological examination showing slight or no autolysis and the presence of ribosomal bands after gel electrophoresis were both indicative parameters of RNA preservation. DNA was appropriate for Southern blotting when the tissue was obtained less than three to five days postmortem, depending on the organ from which the DNA was extracted.
Larsen
S
S
Institute of Pathological Anatomy, University of Copenhagen, Denmark.
Rygaard
K
K
Asnaes
S
S
Spang-Thomsen
M
M
D000311:Adrenal Glands / Q000737:chemistry; D000328:Adult; D000368:Aged; D001344:Autopsy; D015152:Blotting, Northern; D015139:Blotting, Southern; D001923:Brain Chemistry; D004247:DNA / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007668:Kidney / Q000737:chemistry; D008099:Liver / Q000737:chemistry; D008168:Lung / Q000737:chemistry; D008875:Middle Aged; D009206:Myocardium / Q000737:chemistry; D012313:RNA / Q000032:analysis*; D013154:Spleen / Q000737:chemistry
D012313:RNA; D004247:DNA