Isolation and partial characterization of a compound with siderophore activity from Vibrio parahaemolyticus.
A compound with siderophore activity was purified by successive column and thin layer chromatographic procedures from Dowex 1 x 8 extracts of culture supernatants of Vibrio parahaemolyticus AQ 3354. The strain synthesized the compound in culture media containing less than 2 microM added FeCl3. Hydrolysis of the compound yielded alanine, ethanolamine, citric acid and 2-ketoglutaric acid. The 1H-NMR spectrum exhibited the presence of a residue from each of these components in the intact molecule. The fast-atom bombardment mass spectrum of the methyl ester derivative indicated a prominent ion at m/z 477, probably corresponding to [M + 1] ion. Other strains of V. parahaemolyticus were also found to produce this compound when grown in an iron-limited medium.
Yamamoto
S
S
Faculty of Pharmaceutical Sciences, Okayama University, Japan.
Fujita
Y
Y
Okujo
N
N
Minami
C
C
Matsuura
S
S
Shinoda
S
S
D002458:Cell Fractionation; D006868:Hydrolysis; D007502:Iron Chelating Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D017262:Siderophores; D013045:Species Specificity; D014736:Vibrio parahaemolyticus / Q000737:chemistry*
D007502:Iron Chelating Agents; D017262:Siderophores