Dietary regulation of pancreatic amylase in transgenic mice mediated by a 126-base pair DNA fragment.
Expression of the mouse pancreatic amylase gene Amy-2.2 is increased approximately 10-fold in response to increasing the carbohydrate content of the diet from 9.6 to 74%. The DNA sequence mediating this response has been localized to the 5' flanking region of the amylase gene by analysis of hybrid constructs in transgenic mice. The results define a 127-base pair dietary response unit that includes two previously described regulatory elements, an insulin-responsive element and a pancreatic enhancer. Fragments containing these two elements alone fail to respond to diet, demonstrating a requirement for additional regulatory sequences. Another mouse amylase gene Amy-2.1 is only minimally responsive to insulin and to diet. The data are consistent with the hypothesis that the insulin-response element is necessary but not sufficient for regulation of amylase by dietary carbohydrate.
Schmid
R M
RM
Department of Human Genetics, University of Michigan, Ann Arbor 48109-0618.
Meisler
M H
MH
D000681:Amylases / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D004040:Dietary Carbohydrates; D015971:Gene Expression Regulation, Enzymologic; D005796:Genes; D005809:Genes, Regulator; D007527:Isoenzymes / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D010179:Pancreas / Q000201:enzymology*; D016133:Polymerase Chain Reaction / Q000379:methods; D014158:Transcription, Genetic
D004040:Dietary Carbohydrates; D007527:Isoenzymes; D009838:Oligodeoxyribonucleotides; D004247:DNA; D000681:Amylases