title: A straight correlation between mutagenic activity and beta-galactosidase activity induced by monofunctional alkylating agents.
abstract: Eight monofunctional alkylating agents were examined for their ability to induce mutation in Salmonella typhimurium. The assay was carried out in S. typhimurium TA100 with the preincubation method. The SN1-type agents were more mutagenic than the SN2-type ones; besides, methylating agents exerted more mutagenic activity than ethylating ones. Those responses in the reversion assay were quite similar to the results obtained previously with the beta-galactosidase assay in Escherichia coli CSH26/pMCP1000 (alkA'-lacZ') as to the induction of the adaptive response. A good correlation was found between mutagenic potency in the reverse mutation assay and inducing potency in the beta-galactosidase assay.
authors.affiliation: Hita Research Laboratories, Chemicals Inspection and Testing Institute, Oita, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Ohtsuka
authors.forename: K
authors.initials: K
authors.lastname: Maekawa
mesh_terms: D000477:Alkylating Agents / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D016296:Mutagenesis; D009152:Mutagenicity Tests; D009153:Mutagens / Q000494:pharmacology*; D012486:Salmonella typhimurium / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics; D013329:Structure-Activity Relationship; D001616:beta-Galactosidase / Q000378:metabolism*
chemical_list: D000477:Alkylating Agents; D009153:Mutagens; D001616:beta-Galactosidase