title: Analysis of prostatic tumor cultures using fluorescence in-situ hybridization (FISH).
abstract: Analysis of ten primary prostatic tumor cultures using fluorescence in-situ hybridization (FISH) with pericentromeric probes for chromosomes 7, 8, 10, 16, 17, and 18 revealed aneusomies in nine of these specimens. Classical cytogenetics by G-banding indicated that only four of those same ten specimens had any (but not consistent) clonal abnormalities. This preliminary study suggests that aneusomy is a common event in early-stage prostatic tumors, and also supports the notion that multiple chromosomes are involved. In combination with routine cytogenetic analysis, FISH is thus likely to be a powerful tool in the evaluation of prostatic cancer.
authors.affiliation: Department of Pediatrics, Eastern Virginia Medical School, Norfolk.
authors.forename: A R
authors.initials: AR
authors.lastname: Brothman
authors.forename: A M
authors.initials: AM
authors.lastname: Patel
authors.forename: D M
authors.initials: DM
authors.lastname: Peehl
authors.forename: P F
authors.initials: PF
authors.lastname: Schellhammer
mesh_terms: D002869:Chromosome Aberrations; D046508:Culture Techniques; D005069:Evaluation Studies as Topic; D005453:Fluorescence; D006801:Humans; D017403:In Situ Hybridization; D008297:Male; D011471:Prostatic Neoplasms / Q000235:genetics*