title: Growth and production kinetics of human x mouse and mouse hybridoma cells at reduced temperature and serum content.
abstract: The growth and production kinetics of a mouse hybridoma cell line and a human-mouse heterohybridoma were analyzed under conditions of reduced temperature and serum content. The mouse hybridoma P24 had a constant cell specific production rate and RNA content, while the heterohybridoma 3D6-LC4 showed growth associated production kinetics and an increased RNA content at higher growth rates. This behaviour of 3D6-LC4 cells can be explained by the unusual cell cycle kinetics of this line, which can be arrested in any phase under growth limiting conditions, so that a low growth rate does not result in a greater portion of high producing G1-phase cells. Substrate limitation changes the cell cycle distribution of this cell line to a greater extent than low temperature or serum content, which indicates that this stress factor exerts a greater physiological control than assumed.
authors.affiliation: Institute of Applied Microbiology, University of Agriculture, Vienna, Austria.
authors.forename: N
authors.initials: N
authors.lastname: Borth
authors.forename: R
authors.initials: R
authors.lastname: Heider
authors.forename: A
authors.initials: A
authors.lastname: Assadian
authors.forename: H
authors.initials: H
authors.lastname: Katinger
mesh_terms: D000818:Animals; D001790:Blood Physiological Phenomena; D002455:Cell Division; D002460:Cell Line; D003470:Culture Media; D006801:Humans; D006825:Hybridomas / Q000166:cytology*; D007700:Kinetics; D051379:Mice; D012313:RNA / Q000032:analysis; D013696:Temperature
chemical_list: D003470:Culture Media; D012313:RNA