title: Absence of strand-specific repair of cyclobutane pyrimidine dimers in active genes in Drosophila melanogaster Kc cells.
abstract: Strand-specific excision repair of UV-induced cyclobutane pyrimidine dimers was investigated in three genes: Gart, Notch and white in the permanent Kc cell line derived from wild-type Drosophila melanogaster embryonic cells. In this cell line Gart and Notch are transcriptionally active, whereas white is not expressed. Cells were irradiated with 10 or 15 J/m2 ultraviolet (UV) light (predominantly 254 nm). In all three genes, cyclobutane pyrimidine dimers were removed from the non-transcribed strand at the same rate and to the same extent as from the transcribed strand, indicating the absence of strand-specific repair in permanent Drosophila embryonic cell lines.
authors.affiliation: MGC-Department of Radiation Genetics and Chemical Mutagenesis, State University of Leiden, The Netherlands.
authors.forename: J G
authors.initials: JG
authors.lastname: de Cock
authors.forename: E C
authors.initials: EC
authors.lastname: Klink
authors.forename: P H
authors.initials: PH
authors.lastname: Lohman
authors.forename: J C
authors.initials: JC
authors.lastname: Eeken
mesh_terms: D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D004260:DNA Repair; D004331:Drosophila melanogaster / Q000235:genetics*; D015870:Gene Expression; D005796:Genes; D008969:Molecular Sequence Data; D011740:Pyrimidine Dimers / Q000378:metabolism*; D015183:Restriction Mapping; D014158:Transcription, Genetic; D014466:Ultraviolet Rays
chemical_list: D011740:Pyrimidine Dimers