title: Nanogram level quantitation of oxycodone in human plasma by capillary gas chromatography using nitrogen-phosphorus selective detection.
abstract: A sensitive and specific capillary gas chromatographic assay is reported for the quantitation of oxycodone in human plasma. The technique involves a single extraction of oxycodone and internal standard (hydrocodone) from plasma by toluene containing 1% isopropanol. Separation is achieved on a methyl silicone (HP-1) fused-silica capillary column (25 m x 0.2 mm I.D., 0.33 microns film thickness) and detection is by nitrogen-phosphorus selective mode. The minimum quantifiable limit is 1.8 ng/ml using 2 ml of plasma. The method is applicable to characterize the plasma profile of oxycodone in humans after a single oral 5-mg oxycodone hydrochloride tablet.
authors.affiliation: Drug Metabolism and Pharmacokinetics Section, Stine-Haskell Research Center, Du Pont Merck Pharmaceutical Company, Newark, DE 19714.
authors.forename: R P
authors.initials: RP
authors.lastname: Kapil
authors.forename: P K
authors.initials: PK
authors.lastname: Padovani
authors.forename: S Y
authors.initials: SY
authors.lastname: King
authors.forename: G N
authors.initials: GN
authors.lastname: Lam
mesh_terms: D002849:Chromatography, Gas; D006801:Humans; D006853:Hydrocodone / Q000097:blood; D008297:Male; D009584:Nitrogen; D010098:Oxycodone / Q000097:blood*; D010758:Phosphorus; D015203:Reproducibility of Results
chemical_list: D010758:Phosphorus; D006853:Hydrocodone; D010098:Oxycodone; D009584:Nitrogen