Determination of isradipine and its pyridine metabolite in serum by capillary column gas chromatography with nitrogen-selective detection.
A relatively simple, sensitive and precise gas chromatographic method for the determination of isradipine, a calcium antagonist of the dihydropyridine type, and its main metabolite in serum is described. Using a one-step extraction procedure, a wide-bore column and a nitrogen-phosphorus detector, a limit of quantitation of 0.5 and 2.0 nM for isradipine and the metabolite was found. No interferences from several drugs were observed. The method was successfully used in a pharmacokinetic study in hypertensive women during pregnancy.
Christensen
H R
HR
Department of Internal Medicine P, Bispebjerg Hospital, Copenhagen NV, Denmark.
Angelo
H R
HR
Skajaa
K
K
D002121:Calcium Channel Blockers / Q000097:blood / Q000378:metabolism*; D002849:Chromatography, Gas; D004095:Dihydropyridines / Q000097:blood / Q000378:metabolism*; D006801:Humans; D017275:Isradipine; D009584:Nitrogen; D011725:Pyridines / Q000378:metabolism*; D015203:Reproducibility of Results
D002121:Calcium Channel Blockers; D004095:Dihydropyridines; D011725:Pyridines; D009584:Nitrogen; C023666:pyridine; D017275:Isradipine