Size and stability of the genomes of the myxobacteria Stigmatella aurantiaca and Stigmatella erecta.
Genomic DNA of Stigmatella aurantiaca DW 4/3.1 was restricted with the rare-cutting endonucleases AseI and SpeI. The restriction pattern derived is composed of 33 AseI and 25 SpeI fragments, whose total size amounts to approximately 9,350 kbp. Genomic fingerprint analysis of chromosomal DNA from several S. aurantiaca isolates further revealed five completely different SpeI and AseI fingerprints and one distinct fingerprint for Stigmatella erecta. In addition, minor variations between the genome sizes of these isolates were observed.
Neumann
B
B
Zentrum für Molekulare Biologie, University of Heidelberg, Germany.
Pospiech
A
A
Schairer
H U
HU
D002874:Chromosome Mapping; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D016521:Electrophoresis, Gel, Pulsed-Field; D016680:Genome, Bacterial; D009231:Myxococcales / Q000235:genetics*; D013045:Species Specificity
C056436:endodeoxyribonuclease SpeI; C052712:endodeoxyribonuclease VspI; D015252:Deoxyribonucleases, Type II Site-Specific