Inhibition of uptake of 1-methyl-4-phenylpyridinium ion and dopamine in striatal synaptosomes by tobacco smoke components.
To determine whether the attenuation of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced neurotoxicity by tobacco smoke exposure is caused by inhibition of the neuronal uptake of 4-phenylpyridinium ion (MPP+), various tobacco components and a smoke extract were tested for inhibitory activity in striatal synaptosomes. A dimethylsulfoxide extract of tobacco smoke filtrate was found to inhibit the uptake of MPP+ and dopamine. These results suggest that inhibition of the neuronal dopamine uptake mechanism may account for the protective effects of smoke exposure on MPTP-induced neurotoxicity.
Carr
L A
LA
Department of Pharmacology and Toxicology, University of Louisville, KY 40292.
Basham
J K
JK
York
B K
BK
Rowell
P P
PP
D015655:1-Methyl-4-phenylpyridinium / Q000378:metabolism*; D000818:Animals; D003342:Corpus Striatum / Q000187:drug effects / Q000378:metabolism*; D003864:Depression, Chemical; D004298:Dopamine / Q000378:metabolism*; D006834:Hydrazines / Q000494:pharmacology; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009538:Nicotine / Q000494:pharmacology*; D011725:Pyridines / Q000494:pharmacology; D013574:Synaptosomes / Q000378:metabolism*; D014028:Tobacco Smoke Pollution / Q000009:adverse effects / Q000032:analysis*
D006834:Hydrazines; D011725:Pyridines; D014028:Tobacco Smoke Pollution; C029424:hydrazine; C051448:4-phenylpyridine; D009538:Nicotine; D015655:1-Methyl-4-phenylpyridinium; D004298:Dopamine