Gas chromatographic-mass spectrometric method for analysis of a pyrimido-s-triazine and some of its metabolites in dog urine.
3-Phenylpyrimido-[3,4-a]-s-triazines exhibit antiparasitic, antibacterial and antifungal activity. In order to study the metabolism of these heterocycles, 9,9-diethyl-3-phenyl-6,8-dioxo-2,3,4,5,6,7,8,9-octahydropyrimido[3 ,4-a]-s- triazine (TZ) was administered to dogs. Three potential metabolites were synthesized, and these models were identified and quantified with gas chromatography-mass spectrometry. The heterobicyclic compounds, TZ and its hydroxy derivative, underwent thermal degradation under chromatographic conditions. Dog urine spiked with the model metabolites was extracted, and the substances were quantified. The urine of dogs treated with TZ was studied, and two of the potential metabolites were recovered, identified and quantified.
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Laboratoire de Pharmacochimie-Biopharmacie, U.F.R. Médecine-Pharmacie de Rouen, Saint-Etienne du Rouvray, France.
André
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Ménager
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Lafont
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D000818:Animals; D001380:Azauridine / Q000378:metabolism / Q000652:urine*; D001711:Biotransformation; D004285:Dogs; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006863:Hydrogen-Ion Concentration; D008956:Models, Chemical; D015203:Reproducibility of Results; D014227:Triazines / Q000008:administration & dosage*
D014227:Triazines; D001380:Azauridine