Rapid method to isolate serum amyloid P component from human plasma. Characterization of the isolated protein.
A rapid and reproducible method to isolate serum amyloid P component from healthy human plasma has been developed. It uses affinity chromatography on an agarose column followed by anion-exchange chromatography. It was found that the isolated compound has a significantly different isoelectric point (pI 5.7) from that reported previously (pI 4.1). The new data are in good agreement with calculated values determined from the amino acid composition of the protein.
Urbányi
Z
Z
Department of Microbiology, G. Richter Ltd., Budapest, Hungary.
Medzihradszky
D
D
D002846:Chromatography, Affinity; D002852:Chromatography, Ion Exchange; D006801:Humans; D007526:Isoelectric Point; D008970:Molecular Weight; D000683:Serum Amyloid P-Component / Q000737:chemistry / Q000302:isolation & purification*
D000683:Serum Amyloid P-Component