[Characteristics of monoclonal antibodies against chorionic gonadotropin receptor].
We reported the production of monoclonal antibodies (McAb) against chorionic gonadotropin (CG) receptor by fusing spleen cells of BALB/c mice immunized with purified pseudomonas maltophilia CG receptor with mouse myeloma line (SP 2/0). Four hybridoma cell lines secreting CG receptor McAbs were obtained (ED 490, DG 390, AB 890, GE 590). GE 590 is IgG 1; ED 490, DG 390, AB 890 are IgG2b. I125-HCG and ED 490, DG 390. AB 890 recognized CG receptor different antigenic determinant. Increased concentrations of GE 590 were in inverse proportion to the amount of I125-HCG binding to human ovarian tissues showing that normal ovarian tissues and ovarian malignant tumors have different HCG receptor antigenic determinant. This study indicated that these McAbs may be useful in studying the structure of CG receptor and in providing a valuable evidence for early diagnosis and treatment of ovarian malignant tumors.
Han
S
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Department of Obstetrics and Gynecology, 2nd University Hospital, WCUMS Chengdu.
D002288:Adenocarcinoma, Mucinous / Q000276:immunology; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D000939:Epitopes; D005260:Female; D006801:Humans; D006825:Hybridomas / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D010051:Ovarian Neoplasms / Q000276:immunology; D010053:Ovary / Q000276:immunology*; D011974:Receptors, LH / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000939:Epitopes; D011974:Receptors, LH