[Induction of stromal cell transformation in xenografts of human colonic cancer].
Two transformed stromal cell lines FM-7 and FR-7 were obtained from human xenografts of colon tumor (HCT-7), propagated in nude mice and nude rats, respectively. These two cell lines were confirmed to be murine and rat's by a cytogenetic study and were tumourigenic in BALB/c nude mice with the morphology of fibrosarcoma. Human DNA sequences were not detected by hybridization with pBlur8 probe in DNA of FR-7 cell line grown in vitro. These results indicate that human cancer cells from xenograft HCT-7 can induce malignancy in adjacent normal cells of nude mice and nude rats in the absence of DNA transfer.
Bryzgalov
I P
IP
Iudicheva
T V
TV
Galetskiĭ
S A
SA
Solov'ev
Iu N
IuN
Revazova
E S
ES
D000230:Adenocarcinoma / Q000473:pathology*; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D003110:Colonic Neoplasms / Q000473:pathology*; D003582:Cytogenetics; D004247:DNA; D005347:Fibroblasts / Q000473:pathology; D005354:Fibrosarcoma / Q000473:pathology*; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D009693:Nucleic Acid Hybridization; D017154:Stromal Cells / Q000473:pathology*; D014183:Transplantation, Heterologous; D014407:Tumor Cells, Cultured
D004247:DNA