title: Low frequency of rearrangements of the ret and trk proto-oncogenes in Japanese thyroid papillary carcinomas.
abstract: We investigated the frequency of rearrangements of the ret and trk proto-oncogenes in Japanese thyroid tumors. DNAs from 38 thyroid papillary carcinomas and 14 follicular adenomas were analyzed by Southern blotting. Rearrangements of the ret and trk proto-oncogenes were detected in one and two papillary carcinomas, respectively, but not in follicular adenomas. Analysis by a reverse transcriptase-polymerase chain reaction method showed that the ret rearrangement-positive tumor contained the PTC/retTPC chimeric transcript, which was reported to be found specifically in thyroid tumors and adenomatous goiter. We also found that rearranged mRNA of the trk proto-oncogene was expressed at high levels in one of two trk rearrangement-positive tumors. Our results indicated that the frequency of rearrangements of these proto-oncogenes in Japanese papillary carcinomas was much lower than that in Italian patients.
authors.affiliation: Department of Pathology, Nagoya University School of Medicine.
authors.forename: W
authors.initials: W
authors.lastname: Wajjwalku
authors.forename: S
authors.initials: S
authors.lastname: Nakamura
authors.forename: Y
authors.initials: Y
authors.lastname: Hasegawa
authors.forename: K
authors.initials: K
authors.lastname: Miyazaki
authors.forename: Y
authors.initials: Y
authors.lastname: Satoh
authors.forename: H
authors.initials: H
authors.lastname: Funahashi
authors.forename: M
authors.initials: M
authors.lastname: Matsuyama
authors.forename: M
authors.initials: M
authors.lastname: Takahashi
mesh_terms: D000236:Adenoma / Q000235:genetics*; D015152:Blotting, Northern; D002291:Carcinoma, Papillary / Q000235:genetics*; D004273:DNA, Neoplasm / Q000235:genetics; D029721:Drosophila Proteins; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D015321:Gene Rearrangement / Q000235:genetics*; D006801:Humans; D011061:Poly A / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D051096:Proto-Oncogene Proteins c-ret; D011519:Proto-Oncogenes / Q000235:genetics*; D012313:RNA / Q000302:isolation & purification; D012333:RNA, Messenger; D020794:Receptor Protein-Tyrosine Kinases; D020917:Receptor, trkA; D013964:Thyroid Neoplasms / Q000235:genetics*
chemical_list: D004273:DNA, Neoplasm; D029721:Drosophila Proteins; C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D011061:Poly A; D012313:RNA; D051096:Proto-Oncogene Proteins c-ret; D020794:Receptor Protein-Tyrosine Kinases; D020917:Receptor, trkA; C416649:Ret protein, Drosophila