title: The acrosome reaction-inducing activity of individual human follicular fluid samples is highly variable and is related to the steroid content.
abstract: In this study, we have evaluated the relationship between the acrosome reaction-inducing activity of individual human follicular fluid samples and their steroid content. Eighteen samples of follicular fluid were obtained during egg retrieval in six patients undergoing assisted fertilization. Motile spermatozoa were incubated in modified Tyrode's medium (26 mg/ml bovine serum albumin) for 20 h at 1 x 10(7) cells/ml. In a single experiment, aliquots of a semen specimen were simultaneously treated with an aliquot of each follicular fluid sample. The percentage of acrosome reacted spermatozoa was determined using fluorescein isothiocyanate-conjugated Pisum sativum agglutinin (FITC-PSA) lectin. The fluids were also analysed by radioimmunoassay to determine the levels of progesterone, 17 alpha-hydroxy-progesterone, testosterone and oestradiol. The results showed that there was a positive, highly significant correlation between the acrosome reaction-inducing activity and the progesterone level of each follicular fluid sample (r = 0.72, P less than 0.005). Additionally, treatment of the follicular fluid samples with charcoal-dextran caused both a decrease in progesterone concentration and the total loss of the acrosome reaction-inducing activity. The addition of progesterone restored the acrosome reaction-inducing ability in 88% of samples. These data support the idea that progesterone in follicular fluid is the molecule responsible for inducing the acrosome reaction in human spermatozoa.
authors.affiliation: Unit of Reproduction and Development, Faculty of Biological Sciences, P. Catholic University of Chile, Santiago.
authors.forename: P
authors.initials: P
authors.lastname: Morales
authors.forename: M
authors.initials: M
authors.lastname: Llanos
authors.forename: G
authors.initials: G
authors.lastname: Gutierrez
authors.forename: P
authors.initials: P
authors.lastname: Kohen
authors.forename: P
authors.initials: P
authors.lastname: Vigil
authors.forename: D
authors.initials: D
authors.lastname: Vantman
mesh_terms: D000177:Acrosome / Q000502:physiology*; D002606:Charcoal; D003911:Dextrans; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D015571:Follicular Fluid / Q000502:physiology*; D012739:Gonadal Steroid Hormones / Q000378:metabolism*; D006801:Humans; D008297:Male; D011374:Progesterone / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D003911:Dextrans; D012739:Gonadal Steroid Hormones; D002606:Charcoal; D011374:Progesterone