Expression of pp60c-src in human small cell and non-small cell lung carcinomas.
c-src protein was found in 60% of lung carcinomas (20 of 33 cases or primary tumours) by immunoblotting with a monoclonal antibody (Mab 327) and immunohistochemistry with serum from rabbits bearing tumours induced by Rous sarcoma virus. src protein expression was assessed in 4 small cell lung carcinomas and in an atypical carcinoid of neuroendocrine origin. However, pp60c-src was also found in non-small cell lung carcinomas: in 60-80% of adenocarcinomas and bronchiolo-alveolar cancers and in 50% of squamous cell carcinomas. In the squamous cell carcinomas, src protein was expressed more frequently in poorly differentiated than in well and moderately differentiated carcinomas. Expression of pp60c-src was not found in epithelial cells of histologically unchanged lung tissues. These results show that pp60c-src may be activated in human lung carcinomas of different histopathological types.
Mazurenko
N N
NN
Department of Viral Molecular Biology, Cancer Research Center, Moscow, Russia.
Kogan
E A
EA
Zborovskaya
I B
IB
Kisseljov
F L
FL
D000230:Adenocarcinoma / Q000737:chemistry; D002289:Carcinoma, Non-Small-Cell Lung / Q000737:chemistry*; D018288:Carcinoma, Small Cell / Q000737:chemistry*; D002294:Carcinoma, Squamous Cell / Q000737:chemistry; D006801:Humans; D015151:Immunoblotting; D007124:Immunoenzyme Techniques; D008175:Lung Neoplasms / Q000737:chemistry*; D016392:Proto-Oncogene Proteins pp60(c-src) / Q000032:analysis*
D016392:Proto-Oncogene Proteins pp60(c-src)