Differential effect of immunosympathectomy on the expression of rat enteric neurotransmitters.
The effect of immunosympathectomy on the pattern of distribution of catecholamine- and peptide-containing nerve fibres and neurones in the myenteric and submucous plexuses of rat ileum was investigated. There was an increase in vasoactive intestinal polypeptide (VIP)-, galanin (GAL)- and substance P-like immunoreactivity in the myenteric plexus of ileum from rats treated with nerve growth factor (NGF) antiserum compared with controls. A similar increase in immunoreactivity was observed in VIP-, GAL- and neuropeptide Y (NPY)-containing submucous neurones and nerve fibres. In contrast, the immunosympathectomy had no effect on the pattern of distribution of catecholamine-, calcitonin gene-related peptide (CGRP)- and NPY-containing nerve fibres in the myenteric plexus or on substance P- and CGRP-containing neurones and nerve fibres of the submucous plexus. The findings of the present study suggest that NGF may differentially regulate the expression of enteric neuropeptides at a postnatal stage of development.
Belai
A
A
Department of Anatomy and Developmental Biology, University College London, UK.
Aberdeen
J
J
Burnstock
G
G
D000818:Animals; D000831:Animals, Newborn / Q000502:physiology; D002395:Catecholamines / Q000096:biosynthesis; D019004:Galanin; D006651:Histocytochemistry; D007082:Ileum / Q000294:innervation; D009412:Nerve Fibers / Q000276:immunology / Q000378:metabolism; D009414:Nerve Growth Factors / Q000276:immunology / Q000378:metabolism; D009474:Neurons / Q000187:drug effects / Q000378:metabolism; D009479:Neuropeptides / Q000096:biosynthesis; D018377:Neurotransmitter Agents / Q000096:biosynthesis*; D010455:Peptides / Q000276:immunology; D010641:Phenotype; D051381:Rats; D013373:Substance P / Q000276:immunology; D013562:Sympathectomy; D014660:Vasoactive Intestinal Peptide / Q000276:immunology
D002395:Catecholamines; D009414:Nerve Growth Factors; D009479:Neuropeptides; D018377:Neurotransmitter Agents; D010455:Peptides; D013373:Substance P; D014660:Vasoactive Intestinal Peptide; D019004:Galanin