title: Chemotactic response of macrophages to Acanthamoeba castellanii antigen and antibody-dependent macrophage-mediated killing of the parasite.
abstract: The chemotactic potential of antigens of Acanthamoeba castellanii for macrophages and the ability of naive and immune rat peritoneal macrophages to kill A. castellanii in vitro were assessed. The amoebolytic capacity of immune rat serum and complement was also examined. No parasite was killed in the presence of heat-inactivated naive rat serum. Low numbers of parasites were lysed in the presence of heat-inactivated immune rat serum, whereas significantly greater numbers of parasites were lysed in the presence of nonheat-inactivated naive and immune rat serum. Macrophages from naive rats were capable of lysing some parasites. However, the amoebolytic capability of these cells was significantly increased in the presence of serum from immune rats. Regardless of the source of serum used, macrophages from immune rats demonstrated about twice the amoebolytic proficiency of cells from naive rats. Macrophages from naive rats showed their highest capacity for lysing amoebae when incubated in the presence of gamma interferon and immune rat serum. The greatest overall proficiency in lysing parasites was displayed by cells from immune rats incubated with A. castellanii in the presence of gamma interferon and nonheat-inactivated serum from immune rats.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Center for Parasitology, University of Texas, Arlington 76019.
authors.forename: G L
authors.initials: GL
authors.lastname: Stewart
authors.forename: I
authors.initials: I
authors.lastname: Kim
authors.forename: K
authors.initials: K
authors.lastname: Shupe
authors.forename: H
authors.initials: H
authors.lastname: Alizadeh
authors.forename: R
authors.initials: R
authors.lastname: Silvany
authors.forename: J P
authors.initials: JP
authors.lastname: McCulley
authors.forename: J Y
authors.initials: JY
authors.lastname: Niederkorn
mesh_terms: D000048:Acanthamoeba / Q000276:immunology*; D000818:Animals; D000913:Antibodies, Protozoan / Q000276:immunology*; D000953:Antigens, Protozoan / Q000276:immunology*; D002478:Cells, Cultured; D002633:Chemotaxis; D003170:Complement Pathway, Alternative; D003165:Complement System Proteins / Q000276:immunology; D007106:Immune Sera / Q000276:immunology; D007114:Immunization; D008264:Macrophages / Q000276:immunology*; D008297:Male; D010529:Peritoneal Cavity / Q000166:cytology; D051381:Rats; D011917:Rats, Inbred Lew
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D007106:Immune Sera; D003165:Complement System Proteins