title: A nonapeptide encoded by human gene MAGE-1 is recognized on HLA-A1 by cytolytic T lymphocytes directed against tumor antigen MZ2-E.
abstract: We have reported the identification of human gene MAGE-1, which directs the expression of an antigen recognized on a melanoma by autologous cytolytic T lymphocytes (CTL). We show here that CTL directed against this antigen, which was named MZ2-E, recognize a nonapeptide encoded by the third exon of gene MAGE-1. The CTL also recognize this peptide when it is presented by mouse cells transfected with an HLA-A1 gene, confirming the association of antigen MZ2-E with the HLA-A1 molecule. Other members of the MAGE gene family do not code for the same peptide, suggesting that only MAGE-1 produces the antigen recognized by the anti-MZ2-E CTL. Our results open the possibility of immunizing HLA-A1 patients whose tumor expresses MAGE-1 either with the antigenic peptide or with autologous antigen-presenting cells pulsed with the peptide.
authors.affiliation: Ludwig Institute for Cancer Research, Brussels Branch, Belgium.
authors.forename: C
authors.initials: C
authors.lastname: Traversari
authors.forename: P
authors.initials: P
authors.lastname: van der Bruggen
authors.forename: I F
authors.initials: IF
authors.lastname: Luescher
authors.forename: C
authors.initials: C
authors.lastname: Lurquin
authors.forename: P
authors.initials: P
authors.lastname: Chomez
authors.forename: A
authors.initials: A
authors.lastname: Van Pel
authors.forename: E
authors.initials: E
authors.lastname: De Plaen
authors.forename: A
authors.initials: A
authors.lastname: Amar-Costesec
authors.forename: T
authors.initials: T
authors.lastname: Boon
mesh_terms: D000595:Amino Acid Sequence; D000951:Antigens, Neoplasm / Q000235:genetics*; D001483:Base Sequence; D015788:HLA-A1 Antigen / Q000276:immunology*; D006801:Humans; D008545:Melanoma / Q000276:immunology*; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000032:analysis*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
chemical_list: D000951:Antigens, Neoplasm; D015788:HLA-A1 Antigen; D009842:Oligopeptides