Determination of nicotinamide-adenine dinucleotide and thiazole-4-carboxamide-adenine dinucleotide in human leukocytes by reversed-phase high-performance liquid chromatography.
A high-performance liquid chromatographic assay for cellular nicotinamide-adenine dinucleotide and thiazole-4-carboxamide-adenine dinucleotide is presented that is appropriate for analysis of these dinucleotides in extracts of Ficoll-purified human leukemic cells. The separation, which is effected by reversed-phase chromatography, is highly reproducible and the limit of quantitation is as low as 10-15 pmol. The stability of these compounds in neutralized perchloric acid extracts is addressed and the applicability of the procedure to clinical specimens is demonstrated.
Saunders
P P
PP
Department of Medical Oncology, University of Texas M. D. Anderson Cancer Center, Houston 77030.
Alvarez
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E
Kantarjian
H M
HM
D000227:Adenine Nucleotides / Q000032:analysis*; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D007938:Leukemia / Q000097:blood; D007962:Leukocytes / Q000737:chemistry*; D009243:NAD / Q000032:analysis*; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet
D000227:Adenine Nucleotides; D009243:NAD; C038167:thiazole-4-carboxamide adenine dinucleotide