Tumor cell surface-associated binding site for the M(r) 72,000 type IV collagenase.
We have studied the capacity of two human breast adenocarcinoma cells, MDA-MB231 and MCF-7, to bind exogenous M(r) 72,000 type IV collagenase by both morphological and radioreceptor binding assays. By indirect immunofluorescence, staining with a specific anti-M(r) 72,000 type IV collagenase antibody was strongly induced when cells were preincubated with the purified enzyme. Scatchard plot analysis indicated the existence of a binding site for the M(r) 72,000 type IV collagenase with high affinity for both cell lines (Kd = 2 x 10(-9) M). These results are the first demonstration of the existence of a tumor cell membrane-associated putative receptor for a member of the matrix metalloproteinase family, as previously evidenced for the urokinase-type plasminogen activator.
Emonard
H P
HP
Laboratory of Biology, University of Liege, Belgium.
Remacle
A G
AG
Noël
A C
AC
Grimaud
J A
JA
Stetler-Stevenson
W G
WG
Foidart
J M
JM
D000230:Adenocarcinoma / Q000201:enzymology*; D000595:Amino Acid Sequence; D001666:Binding Sites, Antibody; D001943:Breast Neoplasms / Q000201:enzymology*; D017364:Collagenases / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D006801:Humans; D007700:Kinetics; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011869:Radioligand Assay; D014407:Tumor Cells, Cultured
D017364:Collagenases