Large deletions are tolerated at the hprt locus of in vivo derived human T-lymphocytes.
A cloning assay was used to recover hprt- T-lymphocytes from adult human males. Analysis of crude cellular extracts by polymerase chain reactions (PCRs) demonstrated that 7% (16/218) of the hprt mutations were due to total deletion of the hprt gene. 14 of the 16 mutants were examined by PCR for the presence of flanking DNA to determine the extent of the deletions. The deletion mutation in 13 mutants was at least 350 kb with 5 of these deletions being at least 700 kb. The largest deletions were greater than 15 times the size of the hprt gene. Therefore, large deletions are tolerated at the hprt locus of human T-lymphocytes.
Fuscoe
J C
JC
Environmental Health Research and Testing, Inc., Research Triangle Park, NC 27709.
Zimmerman
L J
LJ
Harrington-Brock
K
K
Moore
M M
MM
D000328:Adult; D001483:Base Sequence; D003001:Cloning, Molecular; D017353:Gene Deletion; D005796:Genes / Q000187:drug effects; D006801:Humans; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics*; D008297:Male; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D016133:Polymerase Chain Reaction; D013601:T-Lymphocytes / Q000187:drug effects*; D013866:Thioguanine / Q000633:toxicity; D014960:X Chromosome / Q000187:drug effects*
D007041:Hypoxanthine Phosphoribosyltransferase; D013866:Thioguanine