title: Membrane skeletal protein structure and interactions in human erythrocytes after their treatment with diamide and calcium.
abstract: To analyse the role of native structures of membrane proteins in their structural modifications induced by the elevated intracellular free Ca2+ levels, we have studied the Ca(2+)-mediated effects on membrane skeletal proteins in human erythrocytes that were loaded with Ca2+ using the ionophore A23187 after their pretreatment with the sulphydryl oxidizing agent, diamide. The diamide treatment not only induced polymerization of the major membrane skeletal protein, spectrin, in the erythrocytes, but it also promoted intersubunit crosslinking within the tetramers and dimers of this protein. Loading of these diamide-treated cells with Ca2+ failed to induce significant structural modifications of spectrin as well as polypeptide 4.1, another major membrane skeletal protein, as compared to the erythrocytes that were loaded with Ca2+ without the diamide pretreatment. These results have been interpreted to suggest that the Ca(2+)-induced membrane skeletal protein changes in erythrocytes depend on both the shape and relative orientation of these proteins within the membrane skeleton.
authors.affiliation: Division of Membrane Biology, Central Drug Research Institute, Lucknow.
authors.forename: J
authors.initials: J
authors.lastname: Kumar
authors.forename: C M
authors.initials: CM
authors.lastname: Gupta
mesh_terms: D000199:Actins / Q000097:blood / Q000302:isolation & purification; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000097:blood / Q000494:pharmacology*; D003958:Diamide / Q000494:pharmacology*; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000378:metabolism*; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D046911:Macromolecular Substances; D008565:Membrane Proteins / Q000097:blood* / Q000302:isolation & purification; D013049:Spectrin / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D000199:Actins; D046911:Macromolecular Substances; D008565:Membrane Proteins; D003958:Diamide; D013049:Spectrin; D000001:Calcimycin; D002118:Calcium