A synthetic peptide homologous to retroviral envelope protein down-regulates TNF-alpha and IFN-gamma mRNA expression.
We investigated the influence of CKS-17, a synthetic heptadecapeptide that corresponds to a highly conserved domain of the immunosuppressive retroviral envelope protein p15E, on staphylococcal enterotoxin B (SEB)-induced TNF-alpha gene expression in human peripheral blood mononuclear cells and highly purified human monocyte preparations, as well as the production of TNF-alpha protein, using human peripheral blood mononuclear cells. RNA hybridization studies show that CKS-17 inhibits SEB-induced TNF-alpha mRNA accumulation in human peripheral blood mononuclear cells and human monocytes. CKS-17 is also shown to be highly suppressive for SEB-induced production of TNF-alpha proteins. Similarly, CKS-17 inhibits expression of SEB-induced IFN-gamma mRNA in human peripheral blood mononuclear cells. These results suggest that CKS-17 down-regulates both TNF-alpha and IFN-gamma production at mRNA level.
Haraguchi
S
S
Department of Pediatrics, All Children's Hospital, University of South Florida, St. Petersburg 33701.
Good
R A
RA
Cianciolo
G J
GJ
Day
N K
NK
D000595:Amino Acid Sequence; D015152:Blotting, Northern; D015536:Down-Regulation / Q000187:drug effects*; D004768:Enterotoxins / Q000494:pharmacology; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D015151:Immunoblotting; D036341:Intercellular Signaling Peptides and Proteins; D007371:Interferon-gamma / Q000096:biosynthesis / Q000235:genetics*; D008969:Molecular Sequence Data; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D010455:Peptides / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D015682:Retroviridae Proteins, Oncogenic / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis / Q000235:genetics*; D014759:Viral Envelope Proteins / Q000494:pharmacology*
D004768:Enterotoxins; D036341:Intercellular Signaling Peptides and Proteins; D010455:Peptides; D012333:RNA, Messenger; D015682:Retroviridae Proteins, Oncogenic; D014409:Tumor Necrosis Factor-alpha; D014759:Viral Envelope Proteins; C031818:enterotoxin B, staphylococcal; D007371:Interferon-gamma; C053692:CKS 17