Comparison of two extraction procedures for urinary organic acids prior to gas chromatography-mass spectrometry.
We have compared a new isolation procedure for urinary organic acids using strong anion-exchange columns with a solvent partition (ethyl acetate) method. Urinary samples from two healthy children and from nine children with organic acidurias were analysed by both procedures. Although the solid-phase extraction is more efficient for polyhydroxy acids, some polar acids, and some glycine derivatives, clinically important compounds such as oxalic, methylcitric, pyruvic, glyoxylic and 2-ketoglutaric acids, are not recovered or are only poorly recovered. However, both procedures may be used as a routine method for the diagnosis of the organic acidurias included in this study.
Mardens
Y
Y
Laboratory of Medical Biochemistry, Free University of Brussels (ULB), Belgium.
Kumps
A
A
Planchon
C
C
Wurth
C
C
D000143:Acids / Q000652:urine*; D002648:Child; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007223:Infant; D008661:Metabolism, Inborn Errors / Q000175:diagnosis / Q000652:urine
D000143:Acids