Ultraviolet B-induced DNA fragmentation (apoptosis) in activated T-lymphocytes and Jurkat cells is augmented by inhibition of RNA and protein synthesis.
UVB (280-320 nm) light profoundly affects cellular immunity and immunogenicity. To further characterize the interaction of UVB light with T cells, we examined UVB-induced DNA fragmentation patterns in normal T cells and the T-cell line Jurkat. Resting or preactivated peripheral blood T cells from normal donors or Jurkat cells were exposed to various doses of UVB or gamma irradiation. Cells were sampled at 0-48 h after exposure, and DNA fragmentation was analyzed by electrophoresis in agarose gels. UVB and gamma irradiation, in a dose- and time-dependent manner, induced DNA fragmentation in Jurkat cells and in T cells preactivated with phytohemagglutinin (PHA; 10 micrograms/ml) and phorbol myristate acetate (PMA; 10 ng/ml), but not in resting T cells. The presence of RNA or protein synthesis inhibitors such as actinomycin D or cycloheximide neither inhibited nor delayed DNA fragmentation; in fact, DNA fragmentation was augmented above control values. Similarly, DNA fragmentation increased in the presence of the calcium-chelating agent ethylene-glycol-bis-tetraacetic acid (EGTA) and decreased in the presence of the calcium ionophore A23187. In the presence of ethylenediaminetetraacetic acid (EDTA), DNA fragmentation decreased. In summary, these data show that UVB-induced DNA fragmentation strongly depends upon the cell activation status, and upon the presence of divalent cations other than calcium, possibly magnesium. The data indicate furthermore that in this model, inhibition of RNA or protein synthesis can induce rather than inhibit apoptosis, suggesting that the synthesis inhibitors disrupted primarily the synthesis or action of enzymes ordinarily aimed at repairing DNA fragmentation.
Bazar
L S
LS
Division of Hematology, Georgetown University, Washington, DC.
Deeg
H J
HJ
D002118:Calcium / Q000378:metabolism / Q000494:pharmacology; D002460:Cell Line; D002470:Cell Survival / Q000528:radiation effects; D003513:Cycloheximide / Q000494:pharmacology; D004247:DNA / Q000032:analysis / Q000235:genetics / Q000528:radiation effects*; D004249:DNA Damage; D003609:Dactinomycin / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004307:Dose-Response Relationship, Radiation; D004492:Edetic Acid / Q000494:pharmacology; D006801:Humans; D008213:Lymphocyte Activation / Q000528:radiation effects; D008274:Magnesium / Q000494:pharmacology; D008934:Mitogens / Q000494:pharmacology; D011506:Proteins / Q000378:metabolism*; D012313:RNA / Q000378:metabolism* / Q000528:radiation effects; D013601:T-Lymphocytes / Q000737:chemistry / Q000528:radiation effects*; D013997:Time Factors; D014466:Ultraviolet Rays
D008934:Mitogens; D011506:Proteins; D003609:Dactinomycin; D012313:RNA; D004247:DNA; D003513:Cycloheximide; D004492:Edetic Acid; D008274:Magnesium; D002118:Calcium