Effect of free radicals on excitation-contraction coupling in isolated rat and guinea pig ventricular myocytes.
Using the model of isolated myocytes, we have investigated under current- and/or voltage-clamp conditions: (i) the time-course of H2O2-induced changes in the action potentials and the contractility. (ii) the effect of pharmacological tools such as ryanodine, TTX and nifedipine on H2O2-induced changes; (iii) the effect H2O2 on ionic currents underlying the changes in APD; and (iv) the effect of deferoxamine on H2O2-induced changes.
Horackova
M
M
Department of Physiology and Biophysics, Faculty of Medicine, Dalhousie University Halifax, Nova Scotia, Canada.
Beresewicz
A
A
D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D002478:Cells, Cultured; D006168:Guinea Pigs; D006321:Heart / Q000187:drug effects / Q000502:physiology*; D006861:Hydrogen Peroxide / Q000378:metabolism / Q000494:pharmacology*; D007473:Ion Channels / Q000187:drug effects / Q000502:physiology; D009200:Myocardial Contraction / Q000187:drug effects; D009543:Nifedipine / Q000494:pharmacology; D051381:Rats; D012433:Ryanodine / Q000494:pharmacology; D013481:Superoxides / Q000378:metabolism; D013779:Tetrodotoxin / Q000494:pharmacology
D007473:Ion Channels; D013481:Superoxides; D012433:Ryanodine; D013779:Tetrodotoxin; D006861:Hydrogen Peroxide; D009543:Nifedipine