[Generation and immunochemical characterization of an anti-egg monoclonal antibody specific to Schistosoma japonicum].
Monoclonal antibodies (McAbs) were generated from mice immunized with soluble egg antigen (SEA) of Schistosoma japonicum. Five of which were specific to SEA of S. japonicum. The isotype of these McAbs was IgG1. Immunoblotting showed that the approximate molecule weight of the antigens recognized by the McAbs were 22 kDa, 116 kDa and greater than 200 kDa respectively. At least 3 isomorphs of the 22 kDa antigen recognized by 1E1 were found at pI 4.6-6 with 2-D Western blot. Moreover, immunoprecipitation using 125I-labeled S. japonicum SEA demonstrated that only one band corresponding to 30 kDa was recognized by 1E1.
Xu
A L
AL
Zhejiang Medical University, Hangzhou.
Zhou
X Y
XY
Wang
B F
BF
Harn
D A
DA
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D000947:Antigens, Helminth / Q000276:immunology; D015153:Blotting, Western; D006825:Hybridomas / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D010063:Ovum / Q000276:immunology; D011233:Precipitin Tests / Q000379:methods; D012549:Schistosoma japonicum / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth