Potassium-induced depolarization displaces exogenously incorporated gangliosides from cortical slices.
Freshly diced rat cerebral cortical tissue was incubated with [3H]gangliosides for 30 min, then perfused for 2 h with different physiological solutions. Significantly more labeled (exogenous) gangliosides were displaced when mildly depolarizing concentrations (25 mM) of KCl were included in the perfusion medium. This provides new evidence for an interaction between gangliosides and membrane mechanisms of excitation.
Wolf
L
L
Department of Biology, Simmons College, Boston, MA 02115.
Irwin
L
L
D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002540:Cerebral Cortex / Q000187:drug effects* / Q000378:metabolism; D005732:Gangliosides / Q000378:metabolism*; D066298:In Vitro Techniques; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects; D011188:Potassium / Q000494:pharmacology*; D011869:Radioligand Assay; D051381:Rats; D017207:Rats, Sprague-Dawley
D005732:Gangliosides; D011188:Potassium; D002118:Calcium