Characterization of a HeLa cDNA clone encoding the human SII protein, an elongation factor for RNA polymerase II.
We present the cloning and sequence characterization of a HeLa cDNA encoding the SII transcription elongation factor. This cDNA clone is distinct from those previously isolated from a human kidney cDNA library [Yoo et al., Nucleic Acids Res. 19 (1991) 1073-1079]. Southern analysis suggests that more than one gene may exist for SII in the human genome. A comparison of deduced amino acid sequences for SII-related proteins from a variety of eukaryotes demonstrates very high similarity, especially within the C-terminal domain.
Chen
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Division of Biochemistry and Molecular Biology, University of California, Berkeley 94720.
England
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Kane
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D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D010445:Peptide Elongation Factors / Q000235:genetics*; D012313:RNA / Q000032:analysis; D012319:RNA Polymerase II / Q000502:physiology*; D014157:Transcription Factors / Q000235:genetics*; D035165:Transcription Factors, General; D035602:Transcriptional Elongation Factors
D010445:Peptide Elongation Factors; D014157:Transcription Factors; D035165:Transcription Factors, General; D035602:Transcriptional Elongation Factors; C018648:transcription factor S-II; D012313:RNA; D004247:DNA; D012319:RNA Polymerase II