title: Isoenzyme analysis of Hammondia hammondi and Toxoplasma gondii sporozoites.
abstract: Isoenzyme analysis using isoelectrofocusing in polyacrylamide gels was used to distinguish Hammondia hammondi and Toxoplasma gondii sporozoites. Five enzyme systems were studied: aconitase (EC 4.2.1.3), aspartate aminotransferase (EC 2.6.1.1), glucose phosphate isomerase (EC 5.3.1.9), lactate dehydrogenase (EC 1.1.1.27), and phosphoglucomutase (EC 2.7.5.1). Three stocks of T. gondii belonging to 3 zymodemes were compared to 1 stock of H. hammondi. Hammondia hammondi differed from T. gondii at all 5 loci analyzed. This was observed for all 3 zymodemes of T. gondii. These results indicated clear genetic differences between the 2 species.
authors.affiliation: Service de Parasitologie-Mycologie, CHU Dupuytren, Limoges, France.
authors.forename: M L
authors.initials: ML
authors.lastname: Dardé
authors.forename: H
authors.initials: H
authors.lastname: Riahi
authors.forename: B
authors.initials: B
authors.lastname: Bouteille
authors.forename: M
authors.initials: M
authors.lastname: Pestre-Alexandre
mesh_terms: D000154:Aconitate Hydratase / Q000032:analysis; D000818:Animals; D001219:Aspartate Aminotransferases / Q000032:analysis; D002371:Cat Diseases / Q000469:parasitology; D002415:Cats; D003044:Coccidia / Q000145:classification / Q000201:enzymology* / Q000235:genetics; D003048:Coccidiosis / Q000469:parasitology / Q000662:veterinary; D005956:Glucose-6-Phosphate Isomerase / Q000032:analysis; D007525:Isoelectric Focusing; D007527:Isoenzymes / Q000032:analysis*; D007770:L-Lactate Dehydrogenase / Q000032:analysis; D051379:Mice; D010733:Phosphoglucomutase / Q000032:analysis; D014122:Toxoplasma / Q000145:classification / Q000201:enzymology* / Q000235:genetics; D014124:Toxoplasmosis, Animal / Q000469:parasitology
chemical_list: D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase; D001219:Aspartate Aminotransferases; D000154:Aconitate Hydratase; D005956:Glucose-6-Phosphate Isomerase; D010733:Phosphoglucomutase