title: Characterization of the yeast BMH1 gene encoding a putative protein homologous to mammalian protein kinase II activators and protein kinase C inhibitors.
abstract: We describe the identification and characterization of the BMH1 gene from the yeast Saccharomyces cerevisiae. The gene encodes a putative protein of 292 amino acids which is more than 50% identical with the bovine brain 14-3-3 protein and proteins isolated from sheep brain which are strong inhibitors of protein kinase C. Disruption mutants and strains with the BMH1 gene on multicopy plasmids have impaired growth on minimal medium with glucose as carbon source, i.e. a 30-50% increase in generation time. These observations suggest a regulatory function of the bmh1 protein. In contrast to strains with an intact or a disrupted BMH1 gene, strains with the BMH1 gene on multicopy plasmids hardly grew on media with acetate or glycerol as carbon source.
authors.affiliation: Department of Cell Biology and Genetics, Leiden University, The Netherlands.
authors.forename: G P
authors.initials: GP
authors.lastname: van Heusden
authors.forename: T J
authors.initials: TJ
authors.lastname: Wenzel
authors.forename: E L
authors.initials: EL
authors.lastname: Lagendijk
authors.forename: H Y
authors.initials: HY
authors.lastname: de Steensma
authors.forename: J A
authors.initials: JA
authors.lastname: van den Berg
mesh_terms: D048948:14-3-3 Proteins; D000085:Acetates / Q000378:metabolism; D019342:Acetic Acid; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002417:Cattle; D003001:Cloning, Molecular; D004271:DNA, Fungal / Q000737:chemistry; D004789:Enzyme Activation / Q000187:drug effects; D004926:Escherichia coli / Q000235:genetics; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics*; D005800:Genes, Fungal; D005947:Glucose / Q000378:metabolism; D008969:Molecular Sequence Data; D016296:Mutagenesis; D009419:Nerve Tissue Proteins / Q000737:chemistry; D010957:Plasmids; D011493:Protein Kinase C / Q000037:antagonists & inhibitors*; D011494:Protein Kinases / Q000378:metabolism*; D012313:RNA / Q000378:metabolism; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development; D029701:Saccharomyces cerevisiae Proteins; D012689:Sequence Homology, Nucleic Acid; D012756:Sheep; D014170:Transformation, Genetic; D014446:Tyrosine 3-Monooxygenase
chemical_list: D048948:14-3-3 Proteins; D000085:Acetates; C075852:BMH1 protein, S cerevisiae; D004271:DNA, Fungal; D005656:Fungal Proteins; D009419:Nerve Tissue Proteins; D029701:Saccharomyces cerevisiae Proteins; D012313:RNA; D014446:Tyrosine 3-Monooxygenase; D011494:Protein Kinases; D011493:Protein Kinase C; D005947:Glucose; D019342:Acetic Acid