Role for c-myc in activation-induced apoptotic cell death in T cell hybridomas.
Immature T cells and some T cell hybridomas undergo apoptotic cell death when activated through the T cell receptor complex, a phenomenon that is probably related to antigen induced negative selection of developing T cells. This activation-induced apoptosis depends on active protein and RNA synthesis in the dying cells, although none of the genes required for this process have previously been identified. Antisense oligonucleotides corresponding to c-myc block the constitutive expression of c-Myc protein in T cell hybridomas and interfere with all aspects of activation-induced apoptosis without affecting lymphokine production in these cells. These data indicate that c-myc expression is a necessary component of activation-induced apoptosis.
Shi
Y
Y
Department of Immunology, University of Alberta, Edmonton, Canada.
Glynn
J M
JM
Guilbert
L J
LJ
Cotter
T G
TG
Bissonnette
R P
RP
Green
D R
DR
D000818:Animals; D000945:Antigens, Differentiation, T-Lymphocyte / Q000502:physiology; D001483:Base Sequence; D015153:Blotting, Western; D017252:CD3 Complex; D016923:Cell Death / Q000187:drug effects / Q000235:genetics; D005434:Flow Cytometry; D015870:Gene Expression / Q000187:drug effects; D016762:Genes, fos / Q000502:physiology; D016259:Genes, myc / Q000502:physiology*; D006825:Hybridomas; D008213:Lymphocyte Activation / Q000187:drug effects / Q000502:physiology*; D008969:Molecular Sequence Data; D016376:Oligonucleotides, Antisense / Q000235:genetics / Q000494:pharmacology; D016271:Proto-Oncogene Proteins c-myc / Q000096:biosynthesis; D012313:RNA / Q000096:biosynthesis; D011948:Receptors, Antigen, T-Cell / Q000502:physiology; D013601:T-Lymphocytes / Q000502:physiology*; D014158:Transcription, Genetic
D000945:Antigens, Differentiation, T-Lymphocyte; D017252:CD3 Complex; D016376:Oligonucleotides, Antisense; D016271:Proto-Oncogene Proteins c-myc; D011948:Receptors, Antigen, T-Cell; D012313:RNA