title: Effects of ethanol and its fluorinated analogues on the calcium ATPase of sarcoplasmic reticulum.
abstract: The effects of ethanol, monofluoro- and trifluoroethanol on the hydrolysis of ATP and coupled Ca2+ translocation by sarcoplasmic reticulum Ca(2+)-ATPase (EC 3.6.1.38) were measured. All three alcohols had parallel effects on the enzyme activities at concentrations up to 200 mM, suggesting a similar mechanism of action. 19F nuclear magnetic resonance spectroscopy was used to investigate their site of action in the purified enzyme.
authors.affiliation: Department of Biochemistry, University of Queensland, NSW, Australia.
authors.forename: F C
authors.initials: FC
authors.lastname: Bennett
authors.forename: S D
authors.initials: SD
authors.lastname: Gates
authors.forename: G F
authors.initials: GF
authors.lastname: King
authors.forename: B C
authors.initials: BC
authors.lastname: Shanley
authors.forename: R
authors.initials: R
authors.lastname: Smith
mesh_terms: D000818:Animals; D001665:Binding Sites; D001693:Biological Transport, Active / Q000187:drug effects; D002118:Calcium / Q000378:metabolism; D000252:Calcium-Transporting ATPases / Q000037:antagonists & inhibitors*; D000431:Ethanol / Q000031:analogs & derivatives / Q000494:pharmacology*; D066298:In Vitro Techniques; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D011817:Rabbits; D012519:Sarcoplasmic Reticulum / Q000187:drug effects* / Q000378:metabolism; D014270:Trifluoroethanol / Q000494:pharmacology
chemical_list: D000431:Ethanol; C001236:2-fluoroethanol; D014270:Trifluoroethanol; D000252:Calcium-Transporting ATPases; D002118:Calcium