Sorghum mitochondrial atp6: divergent amino extensions to a conserved core polypeptide.
Sorghum mitochondrial atp6 occurs as one copy in the line Tx398 and as two copies in IS1112C. In IS1112C a repeated sequence diverged within the atp6 open reading frames. The two open reading frames (1137 bp, atp6-1; 1002 bp, atp6-2) share an identical conserved region of 756 bp but are flanked 5' by divergent extensions of 246 (atp6-1) or 381 bp (atp6-2). Tx398 carried only atp6-2. The breakpoint of the repeated sequence of the conserved core region corresponds to the amino acid sequence Ser-Pro-Leu-Asp, which is the amino terminus of the proteolytically processed yeast ATP6. The 5' extensions of atp6-1 and atp6-2 were similar to those of rice and maize, respectively. Each open reading is transcribed, however nuclear background influenced transcriptional patterns of atp6-2 in IS1112C.
Mullen
J A
JA
Department of Plant Pathology, University of Florida, Gainesville 32611.
Pring
D R
DR
Kempken
F
F
Ferguson
J
J
Chase
C D
CD
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D017343:Genes, Plant; D014644:Genetic Variation; D046911:Macromolecular Substances; D008928:Mitochondria / Q000201:enzymology; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D012275:Oryza / Q000201:enzymology / Q000235:genetics; D006109:Poaceae / Q000201:enzymology / Q000235:genetics*; D006180:Proton-Translocating ATPases / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic; D014908:Triticum / Q000201:enzymology / Q000235:genetics; D003313:Zea mays / Q000201:enzymology / Q000235:genetics
D046911:Macromolecular Substances; D004247:DNA; D006180:Proton-Translocating ATPases