title: Axoplasmic transport of horseradish peroxidase in single neurons of the dorsal root ganglion studied in vitro by microinjection.
abstract: The dependence of anterograde axoplasmic transport on cytoskeletal components was investigated using microinjection of horseradish peroxidase (HRP) into the somata of chick dorsal root ganglion cells in vitro. Microinjected HRP was transported anterogradely in the neurites and their branches; this transport was disturbed by colchicine in a drug-dependent and time-dependent manner. Cytochalasin B, a drug that depolymerizes actin, did not inhibit the transport of HRP, despite the formation of local swellings in neurites. The microinjection of polyclonal antibodies directed against tubulin and monoclonal antibodies (mAbs) against 200-kDa neurofilaments disturbed the axoplasmic transport of co-injected HRP, which then exhibited an irregular and discontinuous distribution in the axonal branches. The transport of HRP became discontinuous after the injection of anti-tubulin antibodies and led to the formation of globular deposits of HRP. Polyclonal antibodies against actin and mAbs to 160-kDa and 68-kDa neurofilaments seemed to have no effect on the axoplasmic transport of co-injected HRP. Microinjection of antibodies against tubulin induced formation of perinuclear bundles consisting of cytoskeletal components. The transport of HRP thus appears to be regulated by an intact microtubular system and cross-linker components (200-kDa neurofilaments) of the cytoskeleton. Actin and most intermediate filament proteins do not seem to play an essential role in the transport of HRP.
authors.affiliation: Abteilung für Cytologie, Ruhr-Universität Bochum, Federal Republic of Germany.
authors.forename: K
authors.initials: K
authors.lastname: Meller
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D001370:Axonal Transport; D002642:Chick Embryo; D003078:Colchicine / Q000494:pharmacology; D003571:Cytochalasin B / Q000494:pharmacology; D003599:Cytoskeleton / Q000187:drug effects / Q000502:physiology; D005727:Ganglia, Spinal / Q000166:cytology*; D006735:Horseradish Peroxidase; D008845:Microinjections; D009474:Neurons / Q000648:ultrastructure; D014404:Tubulin / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D014404:Tubulin; D003571:Cytochalasin B; D006735:Horseradish Peroxidase; D003078:Colchicine