title: The use of nylon wool for the isolation of T lymphocyte subpopulations.
abstract: A simple method for the affinity isolation of lymphocyte subpopulations using nylon wool as a solid phase matrix is described. This matrix was coated with an acid-treated IgG fraction of polyclonal antibodies against mouse immunoglobulins. Using this approach, a simple isolation procedure for lymphocyte subpopulations, yielding purities higher than 95% was developed. The method can be used on a large scale for positive and negative cell selection, with very little non-specific binding. The isolated populations are fully functional.
authors.affiliation: Department of Immunology, Hellenic Anticancer Institute, Athens, Greece.
authors.forename: D
authors.initials: D
authors.lastname: Kokkinopoulos
authors.forename: S
authors.initials: S
authors.lastname: Perez
authors.forename: R
authors.initials: R
authors.lastname: Sotiriadou
authors.forename: J
authors.initials: J
authors.lastname: Stinios
authors.forename: M
authors.initials: M
authors.lastname: Papamichail
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D002455:Cell Division; D002469:Cell Separation / Q000379:methods*; D002675:Child, Preschool; D002846:Chromatography, Affinity / Q000379:methods*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007074:Immunoglobulin G; D016130:Immunophenotyping; D007223:Infant; D009757:Nylons; D016176:T-Lymphocyte Subsets / Q000276:immunology / Q000502:physiology
chemical_list: D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D009757:Nylons