The VLA-4/VCAM-1 molecules participate in gamma delta cell interaction with endothelial cells.
The accumulation of T lymphocytes at the site of chronic inflammation depends on a number of factors including adherence of T cells to vascular endothelial cells (EC) and endothelial permeability. We examined the effects of human gamma delta + T lymphocytes on the permeability of EC to macromolecules and characterized the cell surface molecules that are involved in these interactions. In this model, the flux of [125I]albumin was measured across the EC monolayer after a short-term culture with cloned gamma delta cells. Our results show that coculture of activated, but not resting, gamma delta cells with EC enhances endothelial permeability by a cytolytic process. Pretreating gamma delta cells with monoclonal antibodies directed at either LFA-1 or VLA-4 molecules or pretreating EC with monoclonal antibodies directed against either ICAM-1 or VCAM-1 molecules significantly inhibited gamma delta cell-mediated enhancement in endothelial permeability. This indicated that VLA-4/VCAM-1 and LFA-1/ICAM-1 adhesion pathways participate in gamma delta cell-EC interaction.
Mohagheghpour
N
N
Kuzell Institute of Arthritis and Infectious Diseases, San Francisco, California 94115.
Bermudez
L E
LE
Khajavi
S
S
Rivas
A
A
D000945:Antigens, Differentiation, T-Lymphocyte / Q000502:physiology; D018801:CD2 Antigens; D002199:Capillary Permeability; D015815:Cell Adhesion Molecules / Q000502:physiology*; D002450:Cell Communication; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000502:physiology*; D006801:Humans; D016169:Lymphocyte Function-Associated Antigen-1 / Q000502:physiology; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000032:analysis*; D011971:Receptors, Immunologic / Q000502:physiology; D016029:Receptors, Very Late Antigen / Q000502:physiology*; D013601:T-Lymphocytes / Q000502:physiology*; D019010:Vascular Cell Adhesion Molecule-1
D000945:Antigens, Differentiation, T-Lymphocyte; D018801:CD2 Antigens; D015815:Cell Adhesion Molecules; D016169:Lymphocyte Function-Associated Antigen-1; D016692:Receptors, Antigen, T-Cell, gamma-delta; D011971:Receptors, Immunologic; D016029:Receptors, Very Late Antigen; D019010:Vascular Cell Adhesion Molecule-1