Effect of hyperthermia and protein kinase C inhibitors on DNA synthesis and cell proliferation on Ehrlich ascites tumour cells in vitro.
Effect of hyperthermia and/or protein kinase inhibitors on DNA synthesis and cell proliferation was investigated in Ehrlich ascites tumour cells in vitro. Both H-7 and H-8, potent inhibitors of protein kinase C, suppressed DNA synthesis significantly, but HA1004, an inhibitor of cAMP- and cGMP-dependent protein kinase, did not. Hyperthermia increased greatly the suppressive activity of H-7 and H-8 but not that of HA1004. H-7 also inhibited cell growth. These results suggest that the inhibition of protein kinase C enhances the suppression of DNA synthesis and the proliferation of tumour cells by hyperthermia.
Kageyama
K
K
Radioisotope Center, Osaka City University Medical School, Japan.
Onoyama
Y
Y
Matsui-Yuasa
I
I
Otani
S
S
Morisawa
S
S
Kanayama
Y
Y
Takeda
T
T
D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000188:drug therapy* / Q000378:metabolism / Q000628:therapy*; D002455:Cell Division / Q000187:drug effects; D003131:Combined Modality Therapy; D004273:DNA, Neoplasm / Q000096:biosynthesis; D005069:Evaluation Studies as Topic; D005260:Female; D006979:Hyperthermia, Induced; D066298:In Vitro Techniques; D007546:Isoquinolines / Q000627:therapeutic use; D051379:Mice; D008813:Mice, Inbred ICR; D010879:Piperazines / Q000627:therapeutic use; D011493:Protein Kinase C / Q000037:antagonists & inhibitors*; D013449:Sulfonamides
D004273:DNA, Neoplasm; D007546:Isoquinolines; D010879:Piperazines; D013449:Sulfonamides; D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; C053177:N-(2-(methylamino)ethyl)-5-isoquinolinesulfonamide; C043204:N-(2-guanidinoethyl)-5-isoquinolinesulfonamide; D011493:Protein Kinase C