[Detection of minimal residual disease using reverse transcriptase polymerase chain reaction technique].
Polymerase chain reaction (PCR) is a highly sensitive technique to detect minimal residual disease (MRD) of hematological malignancy by amplifying tumor specific nucleotide sequences. When breakpoint is located over large lesions of genomic DNA, like t(9;22) leukemia, PCR amplifying cDNA of chimeric mRNA, called reverse transcriptase PCR (RT-PCR), can be utilized. We applied this method for the detection of MRD in patients with t(1; 19) acute lymphoblastic leukemia. RT-PCR detection of MRD in patients with leukemia might be useful for estimating of the depth of remission, for disclosing preclinical relapse, and for evaluating the efficacy of in vitro purging in autologous bone marrow transplantation.
Numata
S
S
Department of Pediatrics, Nagoya University School of Medicine.
Kato
K
K
Horibe
K
K
D004273:DNA, Neoplasm / Q000032:analysis*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000175:diagnosis*; D012333:RNA, Messenger; D012194:RNA-Directed DNA Polymerase; D014178:Translocation, Genetic
D004273:DNA, Neoplasm; D012333:RNA, Messenger; D012194:RNA-Directed DNA Polymerase