Isolation of a complementary DNA that encodes the mammalian splicing factor SC35.
The mammalian splicing factor SC35 is required for the first step in the splicing reaction and for spliceosome assembly. The cloning and characterization of a complementary DNA encoding this protein revealed that it is a member of a family of splicing factors that includes mammalian SF2/ASF. This family of proteins is characterized by the presence of a ribonucleoprotein (RNP)-type RNA binding motif and a carboxyl-terminal serine-arginine-rich (SR) domain. A search of the DNA sequence database revealed that the thymus-specific exon (ET) of the c-myb proto-oncogene is encoded on the antisense strand of the SC35 gene.
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Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, MA 02138.
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D000595:Amino Acid Sequence; D000818:Animals; D016367:Baculoviridae / Q000235:genetics; D001483:Base Sequence; D001665:Binding Sites; D015152:Blotting, Northern; D002460:Cell Line; D003001:Cloning, Molecular; D003062:Codon; D004247:DNA / Q000737:chemistry / Q000302:isolation & purification*; D005091:Exons; D006801:Humans; D008969:Molecular Sequence Data; D009687:Nuclear Proteins; D011506:Proteins / Q000737:chemistry / Q000235:genetics*; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins / Q000235:genetics; D020598:Proto-Oncogene Proteins c-myb; D012313:RNA / Q000378:metabolism; D012326:RNA Splicing; D012261:Ribonucleoproteins; D012689:Sequence Homology, Nucleic Acid; D000068103:Serine-Arginine Splicing Factors; D014162:Transfection
D003062:Codon; C000715470:MAS1 protein, human; D009687:Nuclear Proteins; D011506:Proteins; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins; D020598:Proto-Oncogene Proteins c-myb; D012261:Ribonucleoproteins; C073288:SRSF2 protein, human; D000068103:Serine-Arginine Splicing Factors; D012313:RNA; D004247:DNA