UV absorption complicates PCR decontamination.
UV irradiation is widely used to inactivate contaminating DNA in PCR. Highly UV-absorbent deoxyribonucleoside triphosphates in PCR mixtures reduce the efficiency of UV decontamination. Optimal decontamination may be achieved by irradiating the PCR mixture without the deoxyribonucleoside triphosphates.
Frothingham
R
R
Duke University Medical Center, Durham, NC 27710.
Blitchington
R B
RB
Lee
D H
DH
Greene
R C
RC
Wilson
K H
KH
D016477:Artifacts; D001483:Base Sequence; D004247:DNA / Q000528:radiation effects*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D014466:Ultraviolet Rays
D004247:DNA