Effects of 1,3-chelation induced by cis-diamminedichloroplatinum(II) on the stability of DNA duplexes.
Several 1,3-intra-strand cross-linked decadeoxynucleotide duplexes, modified with cis-diamminedichloroplatinum(II) (cis-DDP), and their base substitution analogues at the complementary site to the intervening base of the coordination sites, were synthesized and measured for UV-melting profiles to determine melting temperature (Tm) values. The results indicated the thermal stability of the oligonucleotide duplexes containing Pt-induced 1,3-intra-strand cross-linking did not depend on the kind of intervening base of the coordination site but rather on its complementary base. These results may explain the mutagenicity of cis-DDP from a chemical aspect.
Urata
H
H
Osaka University of Pharmaceutical Sciences, Japan.
Tamura
M
M
Urata
M
M
Akagi
M
M
D001483:Base Sequence; D002614:Chelating Agents / Q000494:pharmacology; D002945:Cisplatin / Q000494:pharmacology; D004247:DNA / Q000737:chemistry* / Q000187:drug effects; D004355:Drug Stability; D007700:Kinetics; D008969:Molecular Sequence Data; D009691:Nucleic Acid Denaturation / Q000187:drug effects; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis / Q000737:chemistry*; D013054:Spectrophotometry, Atomic; D013056:Spectrophotometry, Ultraviolet; D013329:Structure-Activity Relationship
D002614:Chelating Agents; D009838:Oligodeoxyribonucleotides; D004247:DNA; D002945:Cisplatin