Use of N-terminal modified poly(L-lysine)-antibody conjugate as a carrier for targeted gene delivery in mouse lung endothelial cells.
A DNA targeted delivery and expression system has been designed based on an N-terminal modified poly(L-lysine) (NPLL)-antibody conjugate, which readily forms a complex with plasmid DNA. Monoclonal antibodies against the cell-surface thrombomodulin conjugated with NPLL were used for targeted delivery of foreign plasmid DNA to an antigen-expressing mouse lung endothelial cell line in vitro and to mouse lungs in vivo. In both cases significant amounts of DNA can be specifically bound to the target cells or tissues. Specific gene expression was observed in the treated mouse lung endothelial cells.
Trubetskoy
V S
VS
Department of Biochemistry, University of Tennessee, Knoxville 37996.
Torchilin
V P
VP
Kennel
S J
SJ
Huang
L
L
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D004247:DNA / Q000187:drug effects / Q000276:immunology*; D004273:DNA, Neoplasm / Q000096:biosynthesis; D004337:Drug Carriers; D004727:Endothelium / Q000166:cytology; D004797:Enzyme-Linked Immunosorbent Assay; D015972:Gene Expression Regulation, Neoplastic; D007457:Iodine Radioisotopes; D008168:Lung / Q000166:cytology; D051379:Mice; D008807:Mice, Inbred BALB C; D010957:Plasmids; D011107:Polylysine / Q000737:chemistry* / Q000276:immunology; D051381:Rats
D000911:Antibodies, Monoclonal; D004273:DNA, Neoplasm; D004337:Drug Carriers; D007457:Iodine Radioisotopes; D011107:Polylysine; D004247:DNA