Deficiency of beta 1-6 N-acetylglucosaminyltransferase involved in the biosynthesis of blood group I antigen in the liver of LEC rats.
The activities of the beta 1-6 and beta 1-3 N-acetylglucosaminyltransferase, which synthesize blood group I and i antigens, respectively, were measured in various tissues of hepatitis- and hepatoma-predisposed rats (LEC rats). In LEC rats the beta 1-6 N-acetylglucosaminyltransferase activity was barely detectable in the liver, while substantial enzyme activity was found in other tissues. In the control LEA rats the enzyme was expressed in most tissues, including the liver. Immunochemical studies using a monoclonal antibody which recognizes I antigen indicated that the expression of I antigen was less prominent in hepatocytes of LEC rats than in hepatocytes of LEA rats. The level of beta 1-3 N-acetylglucosaminyltransferase activity was constant in most of the tissues during the development. These results indicate that the biosynthesis of I antigen does not occur in the livers of the LEC rats.
Gu
J
J
Department of Biochemistry, Osaka University Medical School.
Nishikawa
A
A
Matsuura
N
N
Sugiyama
T
T
Taniguchi
N
N
D000375:Aging / Q000378:metabolism; D000818:Animals; D002240:Carbohydrate Sequence; D005260:Female; D005333:Fetus; D007048:I Blood-Group System; D007150:Immunohistochemistry; D008099:Liver / Q000166:cytology / Q000201:enzymology* / Q000254:growth & development; D008297:Male; D008969:Molecular Sequence Data; D017351:N-Acetylglucosaminyltransferases / Q000172:deficiency* / Q000378:metabolism; D009928:Organ Specificity; D051381:Rats; D011919:Rats, Inbred Strains; D013045:Species Specificity
D007048:I Blood-Group System; D017351:N-Acetylglucosaminyltransferases; C037649:N-acetyllactosaminide beta-1,6-N-acetylglucosaminyltransferase