Determination of tetracyclines in bovine and porcine muscle by high-performance liquid chromatography using solid-phase extraction.
A method is presented for the determination of the three tetracyclines oxytetracycline, tetracycline and chlortetracycline in muscle, spiked at 100 ng/g, using high-performance liquid chromatography (HPLC). The concentration and extraction steps are carried out using Waters Environmental Sep-Pak cartridges. The principal steps involve homogenizing the sample in EDTA-McIlvaine buffer followed by centrifugation and precipitation of the supernatant using trichloroacetic acid. After further filtration and concentration on a Sep-Pak cartridge, the sample is eluted and analysed by HPLC with UV detection and confirmation by diode-array. The column used is a Nova-Pak C18 (4 microns) cartridge (10 cm x 8 mm I.D.). A phosphate-citrate-acetonitrile buffer, utilizing ion suppression, is the mobile phase. The analytes are detectable at levels down to 10 ng/g. The analyte identity can be confirmed at 20 ng/g by the use of diode-array detection and spectral library comparison.
Walsh
J R
JR
Regional Veterinary Laboratory, Department of Agriculture, Hamilton, Victoria, Australia.
Walker
L V
LV
Webber
J J
JJ
D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D008460:Meat / Q000032:analysis*; D009132:Muscles / Q000737:chemistry*; D013552:Swine; D013754:Tetracyclines / Q000032:analysis*
D013754:Tetracyclines