Human cysteine-rich protein. A member of the LIM/double-finger family displaying coordinate serum induction with c-myc.
We previously reported the structure of the placentally derived human cysteine-rich (h crp) cDNA and demonstrated that it encodes a highly conserved and widely distributed zinc finger-like protein. We now report that the expression of both the mouse and human crp genes is induced as a primary response to serum in quiescent Balb/c 3T3 cells and in human fibroblasts. The profile of this primary response is remarkably parallel to that of c-myc in the Balb/c 3T3 cell line. The structure of the 23.2-kilobase h crp gene demonstrates that it is a member of a gene superfamily encoding proteins sharing a highly characteristic 52-amino acid "LIM/double-finger" motif. The evolutionarily conserved structure of cysteine-rich protein, its structural similarity to a number of developmentally critical proteins, its distinctive tissue distribution, and its primary response to early events in the cell cycle suggest that crp plays an important role in cell function.
Wang
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Department of Medicine, University of Pennsylvania, Philadelphia 19104.
Lee
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Liebhaber
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SA
Cooke
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NE
D016475:3T3 Cells; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001769:Blood; D015152:Blotting, Northern; D002453:Cell Cycle; D004247:DNA / Q000235:genetics; D005786:Gene Expression Regulation; D016259:Genes, myc; D006801:Humans; D060588:LIM Domain Proteins; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D005810:Multigene Family; D009687:Nuclear Proteins; D016133:Polymerase Chain Reaction; D011506:Proteins; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics* / Q000378:metabolism; D012313:RNA / Q000235:genetics / Q000378:metabolism; D015183:Restriction Mapping; D014158:Transcription, Genetic; D016335:Zinc Fingers / Q000235:genetics*
C486177:Csrp1 protein, mouse; D060588:LIM Domain Proteins; D009687:Nuclear Proteins; D011506:Proteins; D016271:Proto-Oncogene Proteins c-myc; C074370:cysteine-rich protein, mammalian; D012313:RNA; D004247:DNA