title: Improved method for screening cDNA expression libraries for DNA-binding proteins.
abstract: The ability to successfully screen a lambda gt11 cDNA expression library for specific gene products that can bind to selected sequences of DNA depends on radioactive double-stranded DNA probes with high specific activity. We demonstrate here that probes labeled by the PCR are superior to probes made by the Klenow reaction. The use of these PCR-generated probes have facilitated our efforts to isolate recombinant phage containing putative DNA-binding gene products that recognized a 246-base pair transcriptional enhancer region of Rous sarcoma virus long terminal repeat.
authors.affiliation: Department of Molecular Microbiology and Immunology, University of Missouri-Columbia 65212.
authors.forename: M C
authors.initials: MC
authors.lastname: Harline
authors.forename: J C
authors.initials: JC
authors.lastname: Kandala
authors.forename: R D
authors.initials: RD
authors.lastname: Sage
authors.forename: R V
authors.initials: RV
authors.lastname: Guntaka
authors.forename: A
authors.initials: A
authors.lastname: DeAngelo
mesh_terms: D000818:Animals; D010582:Bacteriophage lambda / Q000235:genetics; D002645:Chickens; D003001:Cloning, Molecular / Q000379:methods*; D004247:DNA / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics*; D015723:Gene Library; D005822:Genetic Vectors; D010957:Plasmids; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D004268:DNA-Binding Proteins; D004247:DNA