title: Human umbilical vein endothelial cells synthesize S-protein (vitronectin) in vitro.
abstract: S-protein, also named vitronectin, is a multifunctional glycoprotein with molecular weight (MW) of about 75 kDa and a serum concentration of 0.14-0.60 mg/ml. It is synthesized mainly in the liver, but synthesis has also been found in monocytes/macrophages. We used human umbilical vein endothelial cells (EC) which were incubated with agarose beads, an activator of the alternative complement pathway. By radioimmunoassay (RIA) based on monoclonal and polyclonal S-protein antibodies, we detected S-protein on harvested agarose beads. The time-dependent increase in the amount of S-protein was significantly reduced by the presence of cycloheximide (10 micrograms/ml) in the cell cultures. We also found a strong binding of S-protein antibodies to agarose beads preincubated in native serum, which was strongly reduced (70-80%) by inactivation of the alternative complement pathway (50 degrees C, 20 min). Our results show that EC synthesize S-protein in vitro.
authors.affiliation: Department of Surgery, Ullevål Hospital, Oslo, Norway.
authors.forename: V
authors.initials: V
authors.lastname: Berge
authors.forename: E
authors.initials: E
authors.lastname: Johnson
authors.forename: K
authors.initials: K
authors.lastname: Høgåsen
authors.forename: G
authors.initials: G
authors.lastname: Hetland
mesh_terms: D002478:Cells, Cultured; D016895:Culture Media, Serum-Free; D004730:Endothelium, Vascular / Q000378:metabolism*; D006023:Glycoproteins / Q000096:biosynthesis*; D006801:Humans; D007118:Immunoassay; D066298:In Vitro Techniques; D019096:Vitronectin
chemical_list: D016895:Culture Media, Serum-Free; D006023:Glycoproteins; D019096:Vitronectin