In-vitro immune response of splenic lymphocytes to portal serum agents from rats undergoing hepatic regeneration or hepatic carcinogenesis.
To clarify the physiologic response of splenic lymphocytes to liver damage and the role of this response in regeneration versus malignant transformation, we cultured rat spleen lymphocytes with portal sera from rats subjected either to partial (70%) hepatectomy or to long-term oral administration of the hepatic carcinogen 3'-methyl-4-dimethylaminoazobenzene. Sera taken within 24h after partial hepatectomy contained a previously described signal protein which serves as a marker of liver damage. The MW 5,000-10,000 serum fraction also contained a factor that promoted cell growth, DNA synthesis, glucose utilization, and the production of anti-sheep erythrocyte plaque-forming cells in cultures of rat splenic lymphocytes. In contrast, the sera of rats subjected to liver damage by the carcinogen had no more effect on the cultured lymphocytes than sera from sham-operated or untreated controls. The signal protein was present initially in portal sera from carcinogen-treated rats, but decreased as hepatitis gave way to cirrhosis. Subsequent malignant transformation was marked by the appearance of serum alpha-fetoprotein. Our results suggest that activation of splenic lymphocytes by serum factor(s) is involved in hepatic regeneration and that this process is deranged in carcinogenesis.
Takahashi
N
N
Division of Agricultural Chemistry, School of Agriculture, Meiji University, Kanagawa, Japan.
Obata
R
R
Sasaki
T
T
D000818:Animals; D000917:Antibody Formation / Q000276:immunology; D001786:Blood Glucose / Q000378:metabolism; D002478:Cells, Cultured; D004247:DNA / Q000032:analysis; D006498:Hepatectomy; D007958:Leukocyte Count / Q000187:drug effects; D008113:Liver Neoplasms / Q000097:blood / Q000139:chemically induced*; D008115:Liver Regeneration / Q000187:drug effects / Q000502:physiology*; D008214:Lymphocytes / Q000276:immunology*; D008297:Male; D008749:Methyldimethylaminoazobenzene / Q000633:toxicity*; D011169:Portal Vein; D051381:Rats; D017207:Rats, Sprague-Dawley; D013154:Spleen / Q000166:cytology / Q000276:immunology*; D013234:Stem Cells / Q000187:drug effects
D001786:Blood Glucose; D008749:Methyldimethylaminoazobenzene; D004247:DNA