The stability and functional properties of proteoliposomes mixed with dextran derivatives bearing hydrophobic anchor groups.
Liposomes composed of Escherichia coli phospholipid were coated with polysaccharides bearing hydrophobic palmitoyl anchors. The effect on the stability of liposomes without or with integral membrane proteins was investigated. A high concentration of hydrophobized dextrans protected the liposomes against detergent degradation, decreased the fluidity of the membranes, prevented fusion of the liposomes and enhanced their stability. Proteoliposomes containing beef heart cytochrome-c oxidase and the lactose transport carrier of E. coli were similarly affected by coating with the dextrans. Under these conditions both membrane proteins were still active. Long-term stability of the coated liposomes was obtained only in the absence of the integral membrane proteins.
Elferink
M G
MG
Department of Microbiology, University of Groningen, Haren, Netherlands.
de Wit
J G
JG
In 't Veld
G
G
Reichert
A
A
Driessen
A J
AJ
Ringsdorf
H
H
Konings
W N
WN
D000818:Animals; D002118:Calcium / Q000378:metabolism; D002240:Carbohydrate Sequence; D002413:Cations, Divalent; D002417:Cattle; D003911:Dextrans / Q000378:metabolism* / Q000494:pharmacology; D003576:Electron Transport Complex IV / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism; D029968:Escherichia coli Proteins; D005452:Fluoresceins; D005454:Fluorescence Polarization; D008081:Liposomes; D008560:Membrane Fluidity / Q000187:drug effects; D008561:Membrane Fusion / Q000187:drug effects; D008564:Membrane Potentials / Q000187:drug effects; D008565:Membrane Proteins / Q000378:metabolism; D026901:Membrane Transport Proteins / Q000378:metabolism; D008969:Molecular Sequence Data; D009004:Monosaccharide Transport Proteins; D009206:Myocardium / Q000201:enzymology; D011510:Proteolipids / Q000378:metabolism*; D027981:Symporters
D002413:Cations, Divalent; D003911:Dextrans; D029968:Escherichia coli Proteins; D005452:Fluoresceins; C050221:LacY protein, E coli; D008081:Liposomes; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D009004:Monosaccharide Transport Proteins; D011510:Proteolipids; D027981:Symporters; C020485:proteoliposomes; C024098:6-carboxyfluorescein; C021499:lactose permease; D003576:Electron Transport Complex IV; D002118:Calcium