The influence of neuraminidase treatment on tracheal smooth muscle contraction.
To examine the role of sialic acid in the respiratory tract, the influence of neuraminidase from Clostridium perfringens was investigated on contractions of isolated guinea-pig and rat trachea and on histamine release from guinea-pig lung tissue. Treatment with 2.0 units/ml of neuraminidase at 37 degrees C and pH 7.4 for 30 min caused an approximately 60% removal of total N-acetylneuraminic acid, a representative sialic acid, from muscle from guinea-pig and rat trachea. Neuraminidase concentration dependently induced histamine release from guinea-pig chopped lung tissue, but has no effect on contractions produced by acetylcholine, histamine and 5-hydroxytryptamine. Pretreatment with 2.0 units/ml of neuraminidase inhibited the contraction induced by antigen (ovalbumin) or compound 48/80. These findings suggest, at least in part, that sialic acids sensitive to neuraminidase are involved in the regulation of histamine release but not tracheal contraction.
Kai
H
H
Department of Pharmacological Sciences, Faculty of Pharmaceutical Sciences, Kumamoto University, Japan.
Makise
K
K
Matsumoto
S
S
Ishii
T
T
Takahama
K
K
Isohama
Y
Y
Miyata
T
T
D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D006168:Guinea Pigs; D006632:Histamine / Q000494:pharmacology; D006636:Histamine Release / Q000187:drug effects; D008168:Lung / Q000187:drug effects / Q000378:metabolism; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects; D009130:Muscle, Smooth / Q000187:drug effects* / Q000502:physiology; D019158:N-Acetylneuraminic Acid; D009439:Neuraminidase / Q000494:pharmacology*; D010047:Ovalbumin / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D012701:Serotonin / Q000494:pharmacology; D012794:Sialic Acids / Q000502:physiology*; D014132:Trachea / Q000187:drug effects* / Q000502:physiology; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology
D012794:Sialic Acids; D012701:Serotonin; D003189:p-Methoxy-N-methylphenethylamine; D006632:Histamine; D010047:Ovalbumin; D009439:Neuraminidase; D019158:N-Acetylneuraminic Acid; D000109:Acetylcholine