Preparation and use of the poly-L-lysine-gold probe: a differential marker of glomerular anionic sites.
The conditions required for the production of a polylysine-coated gold (PL-G) complex, which shows optimal sensitivity for the demonstration of tissue anionic sites, expressed under different conditions of pH have been investigated. Problems encountered with this complex have been compared with those found with other methods of conjugation of polylysine to colloidal gold. The performance of a bovine serum albumin (BSA)-stabilized PL-G complex was examined against other PL-G conjugates, including complexes that are commercially available, for the detection of heterogeneous glomerular anionic site populations, expressed at pH 2.5 and pH 7.0.
Goode
N P
NP
Renal Research Unit, St. James's University N.H.S. Trust, Leeds, UK.
Shires
M
M
Davison
A M
AM
D000838:Anions; D004355:Drug Stability; D006046:Gold; D007150:Immunohistochemistry / Q000379:methods; D007678:Kidney Glomerulus / Q000737:chemistry*; D011107:Polylysine; D012710:Serum Albumin, Bovine; D013194:Staining and Labeling / Q000379:methods
D000838:Anions; D011107:Polylysine; D012710:Serum Albumin, Bovine; D006046:Gold