Characterization of "modified-self"-induced specific antibody hyporesponsiveness to herpes simplex virus.
Pretreatment of mice iv with syngeneic spleen cells modified with soluble HSV envelope antigens induced an anti-HSV antibody hyporesponsiveness following challenge with infectious virus. The epitope density on the HSV-modified spleen cells was quantitated using a photon-counting spectrofluorimeter so that observed immunological results could be correlated with the HSV antigen dose on the splenocytes. The degree of anti-HSV antibody hyporesponsiveness was found to be related to the epitope density on the HSV-modified spleen cells, but not the number of modified cells used in the pretreatment over the 16-fold range tested. Anti-HSV antibody hyporesponsiveness was induced if 7, but not 3, days had elapsed between pretreatment and challenge. This antibody hyporesponsiveness could be adoptively transferred with T cells. Only mice that had induced an anti-HSV antibody hyporesponsiveness following pretreatment with HSV-modified splenocytes were able to survive an LD50 challenge with infectious virus.
Wilson
L A
LA
Department of Microbiology, Immunology, Louisiana State University Medical Center, New Orleans.
Thompson
J J
JJ
Ohkawa
S
S
Scott
D W
DW
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D000956:Antigens, Viral / Q000276:immunology*; D000939:Epitopes; D006561:Herpes Simplex / Q000276:immunology*; D007108:Immune Tolerance; D007928:Lethal Dose 50; D008214:Lymphocytes / Q000276:immunology*; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D018139:Simplexvirus / Q000276:immunology*; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000276:immunology
D000914:Antibodies, Viral; D000956:Antigens, Viral; D000939:Epitopes