Continuous flow synthesis of peptides using a polyacrylamide gel resin (Expansin).
The continuous flow syntheses of endothelin 1, proendothelin 2, ATP binding site of the CDC2 kinase 3, and fragment 18-30 of an actin 4, have been performed by using a polyacrylamide gel resin Expansin (about 0.6 mmol NH2/g) with the glycolamidic ester handle as labile anchorage. In addition, we report here a method of air oxidation which reduces the formation of side-products related to the formation of intermolecular disulfide bridges.
Mendre
C
C
Center of Macromolecular Biochemistry Research, Montpellier, France.
Sarrade
V
V
Calas
B
B
D000180:Acrylic Resins; D000199:Actins / Q000138:chemical synthesis; D000255:Adenosine Triphosphate / Q000378:metabolism; D000595:Amino Acid Sequence; D001665:Binding Sites; D016203:CDC2 Protein Kinase / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D016232:Endothelins / Q000138:chemical synthesis; D005782:Gels; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000138:chemical synthesis; D010455:Peptides / Q000138:chemical synthesis*; D011498:Protein Precursors / Q000138:chemical synthesis
D000180:Acrylic Resins; D000199:Actins; D016232:Endothelins; D005782:Gels; D010446:Peptide Fragments; D010455:Peptides; D011498:Protein Precursors; C016680:polyacrylamide gels; D000255:Adenosine Triphosphate; D016203:CDC2 Protein Kinase