title: In vivo presentation of Mls-1 antigen by T and B lymphocytes.
abstract: Previous studies of minor lymphocyte stimulatory (Mls) presenting lymphoid cells had shown that B cells rather than T cells present stimulatory Mls-1 antigen in vitro whereas B as well as T cells present Mls-1 antigen in vivo. Deletion of Mls-1 reactive T cells in the thymus of newborn mice is induced by T cells rather than by B cells. Applying a recently developed method for measuring the Mls-1 response in Mls-1- mice we assessed the Mls-1 stimulatory activity of T and B cells quantitatively. B cells are significantly more effective than T cells in this process. Both Mls-1+ T and B cells are also capable of inducing Mls-1 anergy in Mls-1- mice. Remarkably few lymphoid cells from Mls-1+ animals are needed for this effect: a few thousand B cells or 10(4) to 10(5) T cells per mouse induce substantial Mls-1 anergy in Mls-1- mice. These low cellular requirements for Mls-1 anergy production correspond well to the low T cell requirements described for the induction of Mls-1 tolerance in newborn mice. However, the high efficacy of B cells in inducing peripheral Mls-1 anergy contrasts with their failure to induce neonatal tolerance in newborn animals. We attribute this discrepancy to the previous notion that stimulatory Mls-1 antigen is not delivered to the thymus and that B cells and T cells present qualitatively different Mls-1 related signals to Mls-1 reactive T cells.
authors.affiliation: New York Medical College, Valhalla.
authors.forename: G
authors.initials: G
authors.lastname: Dannecker
authors.forename: S
authors.initials: S
authors.lastname: Mecheri
authors.forename: M K
authors.initials: MK
authors.lastname: Hoffmann
mesh_terms: D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology* / Q000637:transplantation; D004306:Dose-Response Relationship, Immunologic; D007108:Immune Tolerance / Q000276:immunology; D008198:Lymph Nodes / Q000276:immunology; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D008811:Mice, Inbred DBA; D016747:Minor Lymphocyte Stimulatory Antigens / Q000096:biosynthesis / Q000276:immunology*; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology* / Q000637:transplantation
chemical_list: D016747:Minor Lymphocyte Stimulatory Antigens