LC assay for salmon calcitonin in aerosol formulations using fluorescence derivatization and size exclusion chromatography.
A highly sensitive LC procedure was developed that utilizes fluorescence derivatization and detection coupled with size exclusion chromatography for the analysis of salmon calcitonin in salmon calcitonin aerosols. The LC procedure uses fluorescamine derivatization to label the primary amino groups of the peptide. The derivatization procedure is completely automated by an autosampler capable of pre-column mixing. Size exclusion chromatography is performed using a Supelco G2000 SWXL column. The method can be used to assay the amount of salmon calcitonin delivered per actuation of an aerosol unit. The procedure is simple, accurate, and precise and can detect as little as 2 ng ml-1 concentrations of salmon calcitonin.
Windisch
V
V
Department of Analytical and Physical Chemistry, Rhône-Poulenc Rorer Central Research, Fort Washington, PA 19034.
Karpenko
C
C
Daruwala
A
A
D000336:Aerosols; D000595:Amino Acid Sequence; D000818:Animals; D002116:Calcitonin / Q000032:analysis*; D002853:Chromatography, Liquid; D005450:Fluorescamine / Q000032:analysis; D008969:Molecular Sequence Data
D000336:Aerosols; D005450:Fluorescamine; C028815:salmon calcitonin; D002116:Calcitonin