Effect of bacteriohopane-32-ol on lipid metabolism in Hep G2 cells.
To investigate the biological activity of the hopane group of pentacyclic triterpenes, the effect of bacteriohopane-32-old (Monol) on lipid synthesis and secretion was determined using Hep G2 cells. Despite its structural similarity to 25-hydroxycholesterol, Monol did not affect free and esterified cholesterol synthesis determined by the incorporation from [14C]acetate. Monol reduced the phospholipid secretion from Hep G2 cells without affecting cellular phospholipid synthesis from [3H]glycerol. It also decreased the secretion of apolipoprotein B. These results suggest that the Monol-induced reduction in phospholipid secretion is due to a decrease in the number of lipoprotein particles secreted from Hep G2 cells.
Nagumo
A
A
Central Research Laboratories, Banyu Pharmaceutical Co., Ltd., Tokyo, Japan.
Ishida
F
F
Kamei
T
T
Suzuki
Y
Y
D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology*; D006801:Humans; D050356:Lipid Metabolism; D008114:Liver Neoplasms, Experimental / Q000378:metabolism* / Q000473:pathology; D014315:Triterpenes / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000187:drug effects
D000903:Antibiotics, Antineoplastic; D014315:Triterpenes; C071732:bacteriohopane-32-ol