title: The phylogenetic status of Sarcobium lyticum, an obligate intracellular bacterial parasite of small amoebae.
abstract: A 16S rRNA gene of the obligate intracellular bacterial parasite Sarcobium lyticum was amplified using the polymerase chain reaction in combination with site-specific primers. The amplified DNA was cloned, sequenced and compared with other bacterial 16S rRNA sequences. The analysis revealed that S. lyticum belongs to the gamma subclass of the Proteobacteria and shows the closest relationship to an intracellular Legionella species recovered by amoebal enrichment from the sputum of a patient with pneumonia. S. lyticum could be detected in situ with a fluorescent oligonucleotide probe by whole cell hybridization.
authors.affiliation: Lehrstuhl für Mikrobiologie, Technische Universität München, FRG.
authors.forename: N
authors.initials: N
authors.lastname: Springer
authors.forename: W
authors.initials: W
authors.lastname: Ludwig
authors.forename: W
authors.initials: W
authors.lastname: Drozański
authors.forename: R
authors.initials: R
authors.lastname: Amann
authors.forename: K H
authors.initials: KH
authors.lastname: Schleifer
mesh_terms: D000656:Amoeba / Q000382:microbiology*; D000818:Animals; D001419:Bacteria / Q000235:genetics* / Q000302:isolation & purification; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics; D007875:Legionella / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
chemical_list: D004269:DNA, Bacterial; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S