Formation and activation of a cyclin E-cdk2 complex during the G1 phase of the human cell cycle.
Human cyclin E, originally identified on the basis of its ability to function as a G1 cyclin in budding yeast, associated with a cell cycle-regulated protein kinase in human cells. The cyclin E-associated kinase activity peaked during G1, before the appearance of cyclin A, and was diminished during exit from the cell cycle after differentiation or serum withdrawal. The major cyclin E-associated kinase in human cells was Cdk2 (cyclin-dependent kinase 2). The abundance of the cyclin E protein and the cyclin E-Cdk2 complex was maximal in G1 cells. These results provide further evidence that in all eukaryotes assembly of a cyclin-Cdk complex is an important step in the biochemical pathway that controls cell proliferation during G1.
Koff
A
A
Department of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA 98104.
Giordano
A
A
Desai
D
D
Yamashita
K
K
Harper
J W
JW
Elledge
S
S
Nishimoto
T
T
Morgan
D O
DO
Franza
B R
BR
Roberts
J M
JM
D000818:Animals; D001402:B-Lymphocytes / Q000378:metabolism; D042846:CDC2-CDC28 Kinases; D002460:Cell Line; D051357:Cyclin-Dependent Kinase 2; D018844:Cyclin-Dependent Kinases; D016213:Cyclins / Q000378:metabolism*; D005434:Flow Cytometry; D016193:G1 Phase / Q000502:physiology*; D006801:Humans; D015151:Immunoblotting; D007163:Immunosorbent Techniques; D011494:Protein Kinases / Q000378:metabolism*; D017346:Protein Serine-Threonine Kinases; D051381:Rats
D016213:Cyclins; D011494:Protein Kinases; D017346:Protein Serine-Threonine Kinases; D042846:CDC2-CDC28 Kinases; C495894:CDK2 protein, human; C495892:Cdk2 protein, rat; D051357:Cyclin-Dependent Kinase 2; D018844:Cyclin-Dependent Kinases