Immunomodulatory effects of AS101 on interleukin-2 production and T-lymphocyte function of lymphocytes treated with psoralens and ultraviolet A.
This study tested the immunomodulatory effects of AS101, a synthetic organotellurium compound, on interleukin (IL-2) production and functional activity of normal human lymphocytes. Normal human lymphocytes were treated in vitro with 8-methoxypsoralen alone, ultraviolet A (UVA) and psoralen plus UVA (PUVA) as well as with a combination of AS101 + phytohaemagglutinin (PHA)+phorbol myristate acetate (PMA) and the above-mentioned treatments. Following treatment IL-2 production, free IL-2 receptor (IL-2R), the ability of lymphocytes to induce a local graft-versus-host reaction (GVHR) and the ability of separated CD4 cells to induce help were tested. 8-Methoxypsoralen alone did not significantly affect either cell proliferation or IL-2 production or functional activity. However, UVA and PUVA had a high inhibitory effect on cell proliferation, IL-2 production, IL-2R release and the functional activity of T- and T-helper lymphocytes. In the present study, the addition of AS101 and PMA serve to restore the impaired IL-2 production, T- and T-helper lymphocyte functional activity, but not the IL-2R release. AS101 alone without PMA was also effective in restoring GVHR and helper activity of CD4 lymphocytes, without affecting cell proliferation.
Kozenitzky
L
L
C.A.I.R. Institute, Department of Life Sciences, Bar Ilan University, Ramat Gan, Israel.
David
M
M
Sredai
B
B
Albeck
M
M
Shohat
B
B
D000276:Adjuvants, Immunologic / Q000494:pharmacology; D000818:Animals; D002455:Cell Division / Q000187:drug effects / Q000528:radiation effects; D005030:Ethylenes / Q000494:pharmacology*; D011564:Furocoumarins / Q000494:pharmacology*; D006087:Graft vs Host Reaction; D006801:Humans; D007376:Interleukin-2 / Q000096:biosynthesis*; D011701:PUVA Therapy; D010835:Phytohemagglutinins / Q000494:pharmacology; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D051381:Rats; D011917:Rats, Inbred Lew; D016176:T-Lymphocyte Subsets; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology* / Q000528:radiation effects; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014466:Ultraviolet Rays
D000276:Adjuvants, Immunologic; D005030:Ethylenes; D011564:Furocoumarins; D007376:Interleukin-2; D010835:Phytohemagglutinins; D011837:Radiation-Protective Agents; C054105:ammonium trichloro(dioxoethylene-O,O'-)tellurate; D013755:Tetradecanoylphorbol Acetate