Plasma disappearance of homologous and heterologous pancreatic enzymes in dogs.
Homologous and heterologous (human) pure pancreatic juice was infused into dogs to compare the behaviour of pancreatic enzymes in the circulation. This was achieved by estimating the magnitude of elevation and speed of disappearance of pancreatic enzymes in plasma. The half-lives of the plasma enzymes did not differ significantly between dogs and humans. However the half-lives of other enzymes (longest for amylase and shortest for trypsinogen) did differ. The magnitude of elevation expressed as a ratio of peak (5 min) to basal level was greatest for trypsinogen (28 times) and least for amylase (1.4 times). However, 289% and 410% of the initial lipase enzyme activity was recovered 5 min after infusion of dog and human pure pancreatic juice, respectively (44% when measured by immunoreactive human lipase activity). The excess recovery requires re-evaluation of diagnostic value of turbidimetric activity of plasma pancreatic lipase.
Hayakawa
T
T
Second Department of Internal Medicine, Nagoya University School of Medicine, Japan.
Kondo
T
T
Shibata
T
T
Kitagawa
M
M
Naruse
S
S
D000681:Amylases / Q000008:administration & dosage / Q000097:blood*; D000818:Animals; D004285:Dogs; D006207:Half-Life; D006801:Humans; D008049:Lipase / Q000008:administration & dosage / Q000097:blood*; D010179:Pancreas / Q000201:enzymology*; D014362:Trypsinogen / Q000008:administration & dosage / Q000097:blood*
D014362:Trypsinogen; D008049:Lipase; D000681:Amylases