Calcification in human osteoblasts cultured in medium conditioned by the prostatic cancer cell line PC-3 and prostatic acid phosphatase.
A medium that had been conditioned by PC-3 cells stimulated the calcification of a human osteoblastic cell line, Tak-10, in a nonmitogenic culture. The calcification of the osteoblasts was stimulated maximally at a 25% concentration of the conditioned medium. Calcification activity was markedly enhanced by the addition of both prostatic acid phosphatase (PAP) and its substrate, alpha-glycerophosphate, to the medium; however, PAP added alone did not enhance this activity. These results suggest that human prostatic carcinoma cells produce a factor that stimulates the calcification of the human osteoblasts. Results have also suggested that PAP is a requisite for osteogenesis provided that its substrates are abundant in the medium.
Kimura
G
G
Department of Pathology, Nippon Medical School, Tokyo, Japan.
Sugisaki
Y
Y
Masugi
Y
Y
Nakazawa
N
N
D000135:Acid Phosphatase / Q000502:physiology; D002113:Calcification, Physiologic / Q000502:physiology*; D002455:Cell Division; D003470:Culture Media; D004247:DNA / Q000096:biosynthesis; D005994:Glycerophosphates / Q000502:physiology; D006133:Growth Substances / Q000096:biosynthesis / Q000502:physiology*; D006801:Humans; D008297:Male; D010006:Osteoblasts / Q000737:chemistry / Q000166:cytology / Q000502:physiology* / Q000648:ultrastructure; D010012:Osteogenesis / Q000502:physiology*; D011471:Prostatic Neoplasms / Q000378:metabolism*; D012313:RNA / Q000096:biosynthesis; D014407:Tumor Cells, Cultured
D003470:Culture Media; D005994:Glycerophosphates; D006133:Growth Substances; D012313:RNA; D004247:DNA; C029620:alpha-glycerophosphoric acid; D000135:Acid Phosphatase