title: Novel heme-binding component in the serum of the channel catfish (Ictalurus punctatus).
abstract: The serum of the channel catfish (Ictalurus punctatus) was examined for heme- and hemoglobin-binding proteins. Electrophoretic mobility retardation assays failed to detect a hemoglobin-binding material similar to mammalian haptoglobin; however, a heme-binding component (not previously described) was identified in catfish serum. The heme-binding component was purified by gel filtration chromatography; electrophoretic analyses suggested it to be composed of two polypeptide subunits of molecular masses about 115 and 98 kDa. This composition is inconsistent with hemopexin, the known heme-binding serum protein of mammals. Although it was not fully saturated with heme, the catfish component contained detectable heme in normal sera. When complexed by the binding material, heme was used as an iron source by isolates of the bacterial Gram-negative genus Aeromonas; the capacity of other bacteria to use the complex was not tested. The physiological function of the catfish heme-binding serum protein is presently not clear.
authors.affiliation: Department of Microbiology, University of Mississippi Medical Center, Jackson 39216-4505.
authors.forename: G
authors.initials: G
authors.lastname: Massad
authors.forename: J E
authors.initials: JE
authors.lastname: Arceneaux
authors.forename: B R
authors.initials: BR
authors.lastname: Byers
mesh_terms: D000818:Animals; D001798:Blood Proteins / Q000302:isolation & purification / Q000378:metabolism*; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D006242:Haptoglobins / Q000032:analysis; D006418:Heme / Q000378:metabolism*; D006454:Hemoglobins / Q000378:metabolism*; D007059:Ictaluridae / Q000097:blood*; D007501:Iron / Q000097:blood; D046911:Macromolecular Substances; D008970:Molecular Weight
chemical_list: D001798:Blood Proteins; D006242:Haptoglobins; D006454:Hemoglobins; D046911:Macromolecular Substances; D006418:Heme; D007501:Iron