title: Interaction of the heat shock protein GroEL of Escherichia coli with single-stranded DNA-binding protein: suppression of ssb-113 by groEL46.
abstract: Previous studies from our laboratory have shown that an allele of the heat shock protein GroEL (groEL411) is able to specifically suppress some of the physiological defects of the single-stranded DNA-binding protein mutation ssb-1. A search for additional alleles of the groE genes which may act as suppressors for ssb mutations has led to the identification of groEL46 as a specific suppressor of ssb-113. It has very little or no effect on ssb-1 or ssb-3. All of the physiological defects of ssb-113, including temperature-sensitive growth, temperature-sensitive DNA synthesis, sensitivity to UV irradiation, methyl methanesulfonate, and bleomycin, and reduced recombinational capacity, are restored to wild-type levels. The ssb-113 allele, however, is unable to restore sensitivity of groEL46 cells to phage lambda. The mechanism of suppression of ssb-113 by groEL46 appears to differ from that of ssb-1 by groEL411. The data suggest that GroEL may interact with single-stranded DNA-binding protein in more than one domain.
authors.affiliation: Department of Biological Sciences, University of Cincinnati, Ohio 45221.
authors.forename: P S
authors.initials: PS
authors.lastname: Laine
authors.forename: R R
authors.initials: RR
authors.lastname: Meyer
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D001761:Bleomycin / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D018835:Chaperonin 10; D018834:Chaperonin 60; D004260:DNA Repair / Q000235:genetics; D004269:DNA, Bacterial / Q000096:biosynthesis; D004268:DNA-Binding Proteins / Q000235:genetics*; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics*; D016153:Genes, Suppressor / Q000235:genetics; D005816:Genetic Complementation Test; D006360:Heat-Shock Proteins / Q000235:genetics*; D006358:Hot Temperature; D008741:Methyl Methanesulfonate / Q000494:pharmacology; D009154:Mutation / Q000235:genetics*; D011995:Recombination, Genetic / Q000235:genetics; D013489:Suppression, Genetic; D014466:Ultraviolet Rays
chemical_list: D001426:Bacterial Proteins; D018835:Chaperonin 10; D018834:Chaperonin 60; D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; D006360:Heat-Shock Proteins; D001761:Bleomycin; D008741:Methyl Methanesulfonate