title: Molecular basis for low temperature compartment formation by transitional endoplasmic reticulum of rat liver.
abstract: The molecular basis for temperature compartment formation was investigated using a cell-free system from rat liver. The donor was from liver slices prelabeled with [3H]acetate. Unlabeled Golgi apparatus membranes were immobilized on nitrocellulose as the acceptor. When transfer was determined as a function of temperature, a transition in transfer activity was observed at low temperatures (less than or equal to 20 degrees C) similar to that seen in vivo. The decrease in transfer efficiency correlated with a decrease in phosphatidylethanolamine and phosphatidylserine content of the transition vesicles formed. By adding lipid mixtures enriched in these lipids to the vesicles, their ability to fuse with the cis Golgi apparatus was reconstituted. These findings provide evidence for a role for lipids in low temperature compartment formation.
authors.affiliation: Centre National de la Recherche Scientifique, Institute de Biochimie Cellulaire et de Neurochimie, Bordeaux, France.
authors.forename: P
authors.initials: P
authors.lastname: Moreau
authors.forename: H
authors.initials: H
authors.lastname: Juguelin
authors.forename: C
authors.initials: C
authors.lastname: Cassagne
authors.forename: D J
authors.initials: DJ
authors.lastname: Morré
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001693:Biological Transport, Active; D002451:Cell Compartmentation; D002474:Cell-Free System; D003080:Cold Temperature; D004721:Endoplasmic Reticulum / Q000378:metabolism; D006056:Golgi Apparatus / Q000378:metabolism; D066298:In Vitro Techniques; D007425:Intracellular Membranes / Q000378:metabolism*; D050356:Lipid Metabolism; D008099:Liver / Q000378:metabolism*; D008297:Male; D008561:Membrane Fusion; D010743:Phospholipids / Q000378:metabolism; D051381:Rats
chemical_list: D010743:Phospholipids; D000255:Adenosine Triphosphate