A rapid method for detection of Yersinia enterocolitica serotype O:3 in pig feces using monoclonal antibodies.
A simple colony immunoblotting method using monoclonal antibodies (MAbs) was developed to detect Y. enterocolitica serotype O:3 in pig feces. One of the MAbs studied was able to detect single colonies of the organism in the presence of calculated 3.1 x 10(8) heterologous organisms in pig feces. The MAb was found to be specific for the lipopolysaccharide (LPS) O-antigens of Y. enterocolitica serotype O:3. No significant cross-reactivity was found against a variety of closely related serotypes and Gram-negative organisms. The MAb could also be used in a slide agglutination test and an indirect fluorescence antibody assay for rapid identification of Y. enterocolitica serotype O:3.
Li
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Department of Bioscience and Biotechnology, Drexel University, Philadelphia, PA 19104.
DiSanto
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Magee
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D000372:Agglutination Tests; D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000382:microbiology*; D005455:Fluorescent Antibody Technique; D006825:Hybridomas; D015151:Immunoblotting; D008070:Lipopolysaccharides / Q000276:immunology; D019081:O Antigens; D011135:Polysaccharides, Bacterial / Q000276:immunology; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis*; D015009:Yersinia Infections / Q000175:diagnosis / Q000662:veterinary*; D015008:Yersinia enterocolitica / Q000276:immunology / Q000302:isolation & purification*
D000911:Antibodies, Monoclonal; D008070:Lipopolysaccharides; D019081:O Antigens; D011135:Polysaccharides, Bacterial