Isolation and sequence analysis of the genomic DNA fragment encoding an aspartic proteinase inhibitor homologue from potato (Solanum tuberosum L.).
A genomic DNA clone encoding an aspartic proteinase inhibitor of potato was isolated from a lambda EMBL3 phage library using the aspartic proteinase inhibitor cDNA as a hybridization probe. The gene has all characteristic sequences normally found in eucaryotic genes. Typical CAAT and TATA box sequences were found in the 5'-upstream region. In this part are also two putative regulatory AGGA box sequences located. In the genomic sequence there are no intron sequences interrupting the coding region. An open reading frame of the gene encodes a precursor protein of 217 amino acids which shows high percent identity with the aspartic proteinase inhibitor cDNA.
Maganja
D B
DB
Department of Biochemistry, Jozef Stefan Institute, Ljubljana, Slovenia.
Strukelj
B
B
Pungercar
J
J
Gubensek
F
F
Turk
V
V
Kregar
I
I
D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D002402:Cathepsin D / Q000037:antagonists & inhibitors*; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010940:Plant Proteins; D011506:Proteins / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D011198:Solanum tuberosum / Q000201:enzymology / Q000235:genetics*
D010940:Plant Proteins; D011506:Proteins; C060481:CDI protein, Solanum tuberosum; D004247:DNA; D002402:Cathepsin D