Peptidylglycine alpha-amidating monooxygenase activity and TRH and CRF biosynthesis. Role of copper.
Carboxy-terminal amidation of biologically active peptides, an important characteristic of more than half of these substances, occurs during the maturation process of peptide precursors. It is catalyzed by peptidylglycine alpha-amidating monooxygenase (PAM), an enzyme that is copper-dependent. We show here that alterations of copper stores in cultured cells from different origins (pancreas and hypothalamus) affect the immunoreactivity of thyrotropin-releasing hormone (TRH) and corticotropin-releasing factor (CRF) (two alpha-amidated peptides). This suggests that copper can affect neuropeptide biosynthesis and may play a role in the endocrine or central nervous system function.
Giraud
P
P
Laboratoire de Neuroendocrinologie Expérimentale, INSERM U 297 Faculté de Médecine Nord, Marseille, France.
Maltèse
J Y
JY
Boudouresque
F
F
Salers
P
P
Ouafik
L
L
Renard
M
M
Pelen
F
F
Oliver
C
C
D000595:Amino Acid Sequence; D000818:Animals; D002478:Cells, Cultured; D002614:Chelating Agents / Q000494:pharmacology; D003300:Copper / Q000494:pharmacology*; D003346:Corticotropin-Releasing Hormone / Q000096:biosynthesis* / Q000378:metabolism; D004050:Ditiocarb / Q000494:pharmacology; D005333:Fetus / Q000166:cytology; D007031:Hypothalamus / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D007328:Insulin / Q000378:metabolism; D007515:Islets of Langerhans / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D006899:Mixed Function Oxygenases / Q000378:metabolism*; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes; D051381:Rats; D013973:Thyrotropin-Releasing Hormone / Q000096:biosynthesis* / Q000378:metabolism
D002614:Chelating Agents; D007328:Insulin; D009097:Multienzyme Complexes; D013973:Thyrotropin-Releasing Hormone; D003300:Copper; D003346:Corticotropin-Releasing Hormone; D004050:Ditiocarb; D006899:Mixed Function Oxygenases; C035189:peptidylglycine monooxygenase