Combination of recombinant cytokines fails to produce ex vivo expansion of human blood hematopoietic progenitor cells.
The concept of ex vivo expansion of the human progenitor cell population was tested in this study. In the presence of interleukin-3 and -6 we compared the abilities of various liquid culture conditions of human blood-derived hematopoietic progenitor cells to yield a suitable condition for the expansion of blood progenitor cells. Although the best result was obtained in the gastpermeable bag culture system, the enhancement effect (maximum of 3.8-fold for granulocyte/macrophage colony-forming units and 3.0-fold for erythroid burst-forming units) lasted for only a short period (less than 6 d). The results of this study are disappointing for clinical use, and attempts with currently available technology appear to be limited in their potential.
Takaue
Y
Y
Department of Pediatrics, University Hospital of Tokushima, Japan.
Abe
T
T
Kawano
Y
Y
Hirao
A
A
Yokobayashi
A
A
Okamoto
Y
Y
Saito
S
S
Shimizu
T
T
Sato
J
J
Watanabe
T
T
D000293:Adolescent; D001932:Brain Neoplasms / Q000097:blood*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002648:Child; D002675:Child, Preschool; D003114:Colony-Forming Units Assay; D016207:Cytokines / Q000494:pharmacology*; D004921:Erythropoietin / Q000494:pharmacology; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology; D006412:Hematopoietic Stem Cells / Q000187:drug effects* / Q000473:pathology; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology; D007377:Interleukin-3 / Q000494:pharmacology; D015850:Interleukin-6 / Q000494:pharmacology; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000097:blood*; D011994:Recombinant Proteins / Q000494:pharmacology
D016207:Cytokines; D007377:Interleukin-3; D015850:Interleukin-6; D011994:Recombinant Proteins; D004921:Erythropoietin; D016179:Granulocyte Colony-Stimulating Factor; D007371:Interferon-gamma