Distribution of cells expressing myc proteins in human colorectal epithelium, polyps, and malignant tumors.
The myc gene family encodes nuclear phosphoproteins that are thought to play a role in the control of cellular proliferation and differentiation. We have undertaken an immunohistochemical study assessing the expression of myc gene family proteins in individual cells of normal colonic mucosa, colorectal polyps, and colorectal adenocarcinomas. We screened a panel of mouse monoclonal antibodies that we raised against recombinant human c-myc and N-myc proteins for recognition of myc proteins in paraffin tissue sections. Two of these antibodies, H120C69 and H8C150, were selected for indirect immunoperoxidase staining of tissue sections from 16 normal mucosas, 24 polyps, and 30 adenocarcinomas. In normal colon, about 25% of the cells in the lower one-third of the crypts of Lieberkühn stain for myc-related protein. This distribution resembles that of proliferating cells in the crypt. Benign hyperplastic polyps resemble normal mucosa in their myc staining pattern, with about 25% of the cells positive. In adenomatous polyps, the putative precursors of adenocarcinomas, from 50 to 100% of the cells stain positively for myc protein. In these cases, stained cells extend to the luminal surface, consistent with the previously reported expansion of the proliferation zone in these lesions. All adenocarcinomas examined had increased levels of myc protein relative to normal mucosa. The tumor cells exhibited markedly heterogeneous myc staining patterns, both among different tumors and, in some cases, within a single tumor. Comparison with Ki-67 monoclonal antibody staining indicates that myc protein expression in many tumors is uncoupled from cellular proliferation. Surprisingly, we observed increased numbers of myc-expressing cells and increased levels of myc protein in histologically normal colon directly adjacent to tumor, suggesting that many colorectal carcinomas secrete growth factors that activate gene expression in neighboring normal mucosa.
Melhem
M F
MF
Department of Pathology, University of Pittsburgh School of Medicine, Pennsylvania 15260.
Meisler
A I
AI
Finley
G G
GG
Bryce
W H
WH
Jones
M O
MO
Tribby
I I
II
Pipas
J M
JM
Koski
R A
RA
D000230:Adenocarcinoma / Q000737:chemistry* / Q000473:pathology; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D002467:Cell Nucleus / Q000737:chemistry; D003106:Colon / Q000737:chemistry* / Q000166:cytology / Q000473:pathology; D003110:Colonic Neoplasms / Q000737:chemistry* / Q000473:pathology; D003111:Colonic Polyps / Q000737:chemistry* / Q000473:pathology; D003600:Cytosol / Q000737:chemistry; D005629:Frozen Sections; D006801:Humans; D006965:Hyperplasia; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000737:chemistry* / Q000166:cytology; D019394:Ki-67 Antigen; D009687:Nuclear Proteins / Q000032:analysis; D016612:Paraffin Embedding; D016271:Proto-Oncogene Proteins c-myc / Q000032:analysis*; D012333:RNA, Messenger / Q000032:analysis; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D019394:Ki-67 Antigen; D009687:Nuclear Proteins; D016271:Proto-Oncogene Proteins c-myc; D012333:RNA, Messenger