title: Analysis of a 43-kDa glycoprotein from the intracellular parasitic nematode Trichinella spiralis.
abstract: The L1 larvae of the parasitic nematode Trichinella spiralis invade skeletal muscle and initiate a process that has been interpreted to represent skeletal muscle dedifferentiation. In this process, the infected region of the muscle cell is converted into a unique structure, called the Nurse cell. The nematode T. spiralis can survive for tens of years within the cytoplasm of the Nurse cell and secretes proteins into the cytoplasm that are believed to play a role in mediating the Nurse cell formation or maintenance. We have cloned a cDNA encoding the T. spiralis-derived, 43-kDa secreted protein. Structural analysis of the predicted 344-amino acid sequence revealed an N terminally located signal peptide and a potential helix-loop-helix motif in the main body of the protein. Antibodies raised against the 43-kDa recombinant protein were used in immunocytolocalizations of T. spiralis-infected skeletal muscle sections. These antibodies strongly stained the Nurse cell nuclei and the nematode itself. Specific, though slightly weaker staining also occurred in the Nurse cell cytoplasm. In Western blots, the antibodies react with the 43-kDa protein but also detected at least two other T. spiralis-secreted proteins. DNA hybridizations reveal at least one additional 43-kDa-related sequence encoded in the T. spiralis genome. We conclude that either the 43-kDa protein and/or a closely related 43-kDa family member is secreted into the muscle and translocates to the muscle-derived nuclei. This model may provide insights into the mechanisms involved in Nurse cell formation.
authors.affiliation: Department of Genetics and Development, School of Public Health, Columbia University, New York, New York 10032.
authors.forename: D K
authors.initials: DK
authors.lastname: Vassilatis
authors.forename: D
authors.initials: D
authors.lastname: Despommier
authors.forename: D E
authors.initials: DE
authors.lastname: Misek
authors.forename: R I
authors.initials: RI
authors.lastname: Polvere
authors.forename: A M
authors.initials: AM
authors.lastname: Gold
authors.forename: L H
authors.initials: LH
authors.lastname: Van der Ploeg
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D015153:Blotting, Western; D002467:Cell Nucleus / Q000378:metabolism; D004247:DNA; D006023:Glycoproteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D007150:Immunohistochemistry; D008969:Molecular Sequence Data; D011487:Protein Conformation; D012313:RNA / Q000032:analysis; D014234:Trichinella / Q000378:metabolism*
chemical_list: D006023:Glycoproteins; D012313:RNA; D004247:DNA