Cloning, sequencing, and expression of the Escherichia coli peptide methionine sulfoxide reductase gene.
The gene encoding peptide methionine sulfoxide reductase was cloned from an Escherichia coli genomic library using an oligonucleotide probe based on the amino-terminal sequence of the protein. The nucleotide sequence revealed that the gene codes for a polypeptide of 212 amino acid residues with a calculated molecular weight of 23,314. The protein has been overexpressed in E. coli and is present as a soluble active species.
Rahman
M A
MA
Roche Institute of Molecular Biology, Roche Research Center, Nutley, New Jersey 07110.
Nelson
H
H
Weissbach
H
H
Brot
N
N
D000595:Amino Acid Sequence; D010582:Bacteriophage lambda / Q000235:genetics; D001483:Base Sequence; D003001:Cloning, Molecular; D015342:DNA Probes; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics*; D005798:Genes, Bacterial; D015698:Genomic Library; D007700:Kinetics; D051150:Methionine Sulfoxide Reductases; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D010088:Oxidoreductases / Q000235:genetics* / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism
D015342:DNA Probes; D015345:Oligonucleotide Probes; D011994:Recombinant Proteins; D010088:Oxidoreductases; D051150:Methionine Sulfoxide Reductases; C027219:methionine sulfoxide reductase