title: Guide RNAs for transcripts with developmentally regulated RNA editing are present in both life cycle stages of Trypanosoma brucei.
abstract: RNA editing of several mitochondrial transcripts in Trypanosoma brucei is developmentally regulated. The cytochrome b and cytochrome oxidase II mRNAs are edited in procyclic-form parasites but are primarily unedited in bloodstream forms. The latter forms lack the mitochondrial respiratory system present in procyclic forms. Editing of the NADH dehydrogenase 7 (ND7) and ND8 transcripts is also developmentally regulated but occurs preferentially in bloodstream forms. Other transcripts, cytochrome oxidase III and ATPase 6, are edited in both life forms. We have identified many minicircle-encoded guide RNAs (gRNAs) for ATPase 6, ND7, and ND8. The characteristics of these gRNAs reveal how extensively edited RNA can be edited in the 3'-to-5' direction. Northern (RNA) blot and primer extension analyses indicate that gRNAs for transcripts whose editing is developmentally regulated are present in both procyclic and bloodstream form parasites. These results suggest that the developmental regulation of editing in these transcripts is not controlled by the presence or absence of gRNAs.
authors.affiliation: Seattle Biomedical Research Institute, Washington 98109.
authors.forename: D J
authors.initials: DJ
authors.lastname: Koslowsky
authors.forename: G R
authors.initials: GR
authors.lastname: Riley
authors.forename: J E
authors.initials: JE
authors.lastname: Feagin
authors.forename: K
authors.initials: K
authors.lastname: Stuart
mesh_terms: D000375:Aging; D000818:Animals; D001483:Base Sequence; D003573:Cytochrome b Group / Q000235:genetics; D004272:DNA, Mitochondrial / Q000235:genetics*; D003576:Electron Transport Complex IV / Q000235:genetics; D005786:Gene Expression Regulation; D005796:Genes; D008928:Mitochondria / Q000378:metabolism; D008969:Molecular Sequence Data; D009245:NADH Dehydrogenase / Q000235:genetics*; D009838:Oligodeoxyribonucleotides; D012313:RNA / Q000235:genetics; D017394:RNA, Guide, Kinetoplastida; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D000077278:RNA, Mitochondrial; D016053:RNA, Protozoan / Q000235:genetics* / Q000378:metabolism; D051381:Rats; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic; D014346:Trypanosoma brucei brucei / Q000235:genetics* / Q000254:growth & development
chemical_list: D003573:Cytochrome b Group; D004272:DNA, Mitochondrial; D009838:Oligodeoxyribonucleotides; D012333:RNA, Messenger; D000077278:RNA, Mitochondrial; D016053:RNA, Protozoan; D012313:RNA; D009245:NADH Dehydrogenase; D003576:Electron Transport Complex IV; D017394:RNA, Guide, Kinetoplastida