D-glyceraldehyde-3-phosphate dehydrogenase purified from rabbit muscle contains phosphotyrosine.
Homogeneous preparations of D-glyceraldehyde-3-phosphate dehydrogenase purified from rabbit muscle were found to contain 0.2-0.7 moles of covalently bound phosphate per mole of the enzyme. With the use of anti-phosphotyrosine antibodies, evidence was obtained that the enzyme is phosphorylated at tyrosine residues.
Sergienko
E A
EA
A.N. Belozersky Institute of Physico-Chemical Biology, School of Biology, Moscow State University, Russia.
Kharitonenkov
A I
AI
Bulargina
T V
TV
Muronetz
V V
VV
Nagradova
N K
NK
D000818:Animals; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D015151:Immunoblotting; D009132:Muscles / Q000201:enzymology*; D010766:Phosphorylation; D019000:Phosphotyrosine; D011817:Rabbits; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000032:analysis
D019000:Phosphotyrosine; D014443:Tyrosine; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases