Ethylene dibromide: negative results with the mouse dominant lethal assay and the electrophoretic specific-locus test.
Ethylene dibromide (1,2-dibromoethane; EDB) was tested for the induction of dominant lethal and electrophoretically-detectable specific-locus mutations in the germ cells of DBA/2J male mice. Males were treated with a single intraperitoneal injection of 100 mg/kg EDB and mated to two C57BL/6J females. In the dominant lethal assay, matings were carried out to measure the effect of EDB on meiotic and postmeiotic stages; germ cells representing spermatogonial stem cells were analyzed in the electrophoretic specific-locus test. Neither of these germ cell tests produced any evidence that EDB is a germ cell mutagen. It appears from these data and those reported in the literature that EDB, a genotoxic carcinogen that affects male fertility in some mammalian species, is not mutagenic in the germ cells of the male mouse.
Barnett
L B
LB
Research Triangle Institute, Research Triangle Park, NC 27709-2194.
Lovell
D P
DP
Felton
C F
CF
Gibson
B J
BJ
Cobb
R R
RR
Sharpe
D S
DS
Shelby
M D
MD
Lewis
S E
SE
D000818:Animals; D015946:Ethylene Dibromide / Q000633:toxicity*; D005298:Fertility / Q000187:drug effects; D005799:Genes, Dominant; D005804:Genes, Lethal; D008297:Male; D051379:Mice; D009152:Mutagenicity Tests
D015946:Ethylene Dibromide