title: Site-specific mutagenesis of almost any plasmid using a PCR-based version of unique site elimination.
authors.affiliation: Department of Cancer Biology, Harvard School of Public Health, Boston, MA 02115.
authors.forename: F A
authors.initials: FA
authors.lastname: Ray
authors.forename: J A
authors.initials: JA
authors.lastname: Nickoloff
mesh_terms: D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D010957:Plasmids; D016133:Polymerase Chain Reaction
chemical_list: D004269:DNA, Bacterial