23Na and 1H NMR studies on melittin channels activated by tricyclic tranquilizers.
A dynamic 23Na nuclear magnetic resonance (NMR) technique was applied to the exchange system of Na+ ions present inside and outside large unilamellar vesicles at an equivalent concentration. Addition of melittin to phosphatidylcholine vesicles did not induce any detectable Na+ transport across the membrane but subsequent addition of a trace of chlorpromazine or imipramine did induce Na+ transport. Because the formation of a drug-melittin adduct in a solution was detected by 1H NMR, the activation of melittin channels was assumed to originate from the direct interaction of the drug and melittin.
Tanaka
H
H
Faculty of Pharmaceutical Sciences, Josai University, Saitama, Japan.
Matsunaga
K
K
Kawazura
H
H
D001692:Biological Transport; D002746:Chlorpromazine; D006859:Hydrogen; D007099:Imipramine; D007473:Ion Channels / Q000502:physiology*; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008555:Melitten / Q000737:chemistry*; D008954:Models, Biological; D011487:Protein Conformation; D012964:Sodium
D007473:Ion Channels; D008555:Melitten; D006859:Hydrogen; D012964:Sodium; D007099:Imipramine; D002746:Chlorpromazine