title: Heterogeneity of 3'untranslated region of bovine acidic FGF transcripts.
abstract: A bovine aFGF genomic clone (14.2 Kb) has been isolated and characterized. This clone contains exons 2 and 3 interrupted by 6.7 Kb long intron. Exon 3 contains part of the coding region and the 3' untranslated region (3'UTR). Two overlapping cDNA clones specific for this 3'UTR have been isolated from bovine retina cDNA libraries or after amplification of RNA by the RACE technique. Analysis of these clones and RNAse protection assay demonstrate alternative termination of aFGF transcripts giving rise to differently sized 3'UTR of 2.5 Kb and at least 3.9 Kb. The sequence of these long 3'UTR is highly conserved among species (70% identity between human and rat) which suggests an important role for aFGF expression.
authors.affiliation: Unité de Recherches Gérontologiques, INSERM U118, Paris, France.
authors.forename: F
authors.initials: F
authors.lastname: Renaud
authors.forename: S
authors.initials: S
authors.lastname: Desset
authors.forename: K
authors.initials: K
authors.lastname: Bugra
authors.forename: C
authors.initials: C
authors.lastname: Halley
authors.forename: J M
authors.initials: JM
authors.lastname: Philippe
authors.forename: Y
authors.initials: Y
authors.lastname: Courtois
authors.forename: M
authors.initials: M
authors.lastname: Laurent
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002417:Cattle; D003001:Cloning, Molecular; D005091:Exons; D016220:Fibroblast Growth Factor 1 / Q000235:genetics*; D015723:Gene Library; D007438:Introns; D008969:Molecular Sequence Data; D011061:Poly A / Q000235:genetics / Q000302:isolation & purification; D011110:Polymorphism, Genetic; D014176:Protein Biosynthesis; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012333:RNA, Messenger; D015183:Restriction Mapping; D012160:Retina / Q000502:physiology; D014158:Transcription, Genetic
chemical_list: D012333:RNA, Messenger; D016220:Fibroblast Growth Factor 1; D011061:Poly A; D012313:RNA