The effect of glycerol and 4-nitroquinoline 1-oxide on active oxygen formation in sub-cellular fractions of lung tissue.
Glycerol enhances pulmonary tumorigenesis in mice treated with 4-nitroquinoline 1-oxide (4NQO). The present study shows that active oxygen formation by glycerol may be responsible for this enhanced tumorigenesis. Male ddY mice were treated with 4NQO and given a 5% glycerol solution instead of drinking water for up to 4 weeks after 4NQO injection. There was no difference in NADH-dependent active oxygen formation of the mitochondria between the 4NQO- and 4NQO-plus-glycerol-treated groups but the ratios of NADH- and NADPH-dependent active oxygen formation of the microsomes and nuclei of the 4NQO-plus-glycerol-treated group to the 4NQO-treated group increased with increasing time after 4NQO injection. Significant differences in the maximum NADH- and NADPH-dependent active oxygen formation were observed 2 or 4 weeks after 4NQO injection.
Yano
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Division of Applied Food Research, National Institute of Health and Nutrition, Tokyo, Japan.
Ichikawa
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D015112:4-Nitroquinoline-1-oxide / Q000633:toxicity*; D000818:Animals; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism; D003043:Cocarcinogenesis; D005990:Glycerol / Q000494:pharmacology*; D008168:Lung / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D008297:Male; D051379:Mice / Q000235:genetics; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D009243:NAD / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism*; D013047:Specific Pathogen-Free Organisms
D009243:NAD; D009249:NADP; D015112:4-Nitroquinoline-1-oxide; D005990:Glycerol; D010100:Oxygen