title: The 93-kilodalton protein of Borrelia burgdorferi: an immunodominant protoplasmic cylinder antigen.
abstract: Using immunoblots, we identified proteins of Borrelia burgdorferi recognized by sera from 62 patients with either acute or chronic Lyme disease. In all groups studied, the 41-kDa flagellar protein and a relatively minor 93-kDa protein (p93) were the most commonly recognized antigens in patients with acute and chronic disease due to B. burgdorferi. A murine monoclonal antibody (MAb 181.1) was developed against p93, and the antigen was detected by immunoblot analysis in four European and American strains of B. burgdorferi. On two-dimensional gel electrophoresis, p93 had an apparent pI of 6.8. Immunoelectronmicroscopy with MAb 181.1 demonstrated that p93 is located within the protoplasmic cylinder compartment of the organism. The gene encoding p93 was retrieved from a phage expression library. The derived amino acid sequence of p93 confirmed chemical characterization of the antigen, including its amino-terminal peptide sequence. The derived amino acid sequence predicted it to be predominantly alpha helical. A prominent antigenic domain located at the carboxy portion of the protein was recognized by human and rabbit polyclonal antisera and human (MAb D4) and mouse (MAb 181.1) MAbs.
authors.affiliation: Department of Medicine, State University of New York, Stony Brook 11794-8153.
authors.forename: B J
authors.initials: BJ
authors.lastname: Luft
authors.forename: S
authors.initials: S
authors.lastname: Mudri
authors.forename: W
authors.initials: W
authors.lastname: Jiang
authors.forename: R J
authors.initials: RJ
authors.lastname: Dattwyler
authors.forename: P D
authors.initials: PD
authors.lastname: Gorevic
authors.forename: T
authors.initials: T
authors.lastname: Fischer
authors.forename: P
authors.initials: P
authors.lastname: Munoz
authors.forename: J J
authors.initials: JJ
authors.lastname: Dunn
authors.forename: W H
authors.initials: WH
authors.lastname: Schubach
mesh_terms: D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000276:immunology; D000942:Antigens, Bacterial / Q000032:analysis*; D001426:Bacterial Proteins / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D001483:Base Sequence; D015748:Borrelia burgdorferi Group / Q000276:immunology*; D006801:Humans; D008969:Molecular Sequence Data
chemical_list: D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D001426:Bacterial Proteins