Analysis of mixed human/microbial DNA samples: a validation study of two PCR AMP-FLP typing methods.
The reliability of the PCR technique used to type two human variable number tandem repeats, that is, 3' to apolipoprotein B gene and locus D17S30, was examined using DNA samples of mixed human and microbial origin. Mixtures of human and microbial DNA were amplified, choosing microbes found commonly in the vagina. Total inhibition of human amplification and/or "drop-out" of the larger amplification fragment length polymorphism allele was observed at both loci in the presence of DNA from some vaginal micro-flora.
Lienert
K
K
Flinders University of South Australia.
Fowler
J C
JC
D016477:Artifacts; D001483:Base Sequence; D004247:DNA / Q000032:analysis*; D004269:DNA, Bacterial / Q000032:analysis*; D005260:Female; D005554:Forensic Medicine / Q000379:methods*; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D011110:Polymorphism, Genetic; D011902:Rape; D013045:Species Specificity; D014621:Vagina / Q000382:microbiology
D004269:DNA, Bacterial; D004247:DNA