Molecular and microscopic identification of sulfate-reducing bacteria in multispecies biofilms.
The population architecture of sulfidogenic biofilms established in anaerobic fixed-bed bioreactors was characterized by selective polymerase chain reaction amplification and fluorescence microscopy. A region of the 16S rRNA common to resident sulfate-reducing bacteria was selectively amplified by the polymerase chain reaction. Sequences of amplification products, with reference to a collection of 16S rRNA sequences representing most characterized sulfate-reducing bacteria, were used to design both general and specific hybridization probes. Fluorescent versions of these probes were used in combination with fluorescence microscopy to visualize specific sulfate-reducing bacterial populations within developing and established biofilms.
Amann
R I
RI
Department of Veterinary Pathobiology, University of Illinois, Urbana 61801.
Stromley
J
J
Devereux
R
R
Key
R
R
Stahl
D A
DA
D001483:Base Sequence; D015342:DNA Probes; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010084:Oxidation-Reduction; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S / Q000032:analysis / Q000235:genetics*; D013431:Sulfates / Q000378:metabolism*; D016999:Sulfur-Reducing Bacteria / Q000145:classification / Q000302:isolation & purification* / Q000378:metabolism; D013696:Temperature
D015342:DNA Probes; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D013431:Sulfates