cDNA cloning of a neural visinin-like Ca(2+)-binding protein.
A 21,000-dalton Ca(2+)-binding protein (Walsh, M.P., Valentine, K.A., Ngai, P.K., Carruthers, C.A., and Hollengerg, M.D. (1984) Biochem. J. 224, 117-127) was purified from the rat brain and through the use of oligonucleotide probe based on partial amino acid sequence, cDNA clones were obtained from rat brain cDNA library. The complete amino acid sequence deduced from the cDNA contains 191 residues and has a calculated molecular mass of 22,142 daltons. There are three potential Ca(2+)-binding sites like the EF hands in the sequence. It displays striking sequence homology with visinin and recoverin, retina-specific Ca(2+)-binding proteins. Northern blot analysis revealed that the protein is highly and specifically expressed in the brain.
Kuno
T
T
Department of Pharmacology, Kobe University School of Medicine, Japan.
Kajimoto
Y
Y
Hashimoto
T
T
Mukai
H
H
Shirai
Y
Y
Saheki
S
S
Tanaka
C
C
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D001921:Brain / Q000502:physiology*; D002135:Calcium-Binding Proteins / Q000235:genetics* / Q000302:isolation & purification; D003001:Cloning, Molecular / Q000379:methods; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D005136:Eye Proteins; D015723:Gene Library; D051597:Hippocalcin; D008074:Lipoproteins; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009419:Nerve Tissue Proteins / Q000235:genetics*; D009838:Oligodeoxyribonucleotides; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D051381:Rats; D051603:Recoverin; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
D002135:Calcium-Binding Proteins; D005136:Eye Proteins; D008074:Lipoproteins; D009419:Nerve Tissue Proteins; D009838:Oligodeoxyribonucleotides; C496733:Rcvrn protein, rat; C045140:visinin; D051603:Recoverin; D051597:Hippocalcin; D012313:RNA; D004247:DNA