Autoradiography of lymph nodes with 99mTc-dextran in rabbits.
99mTc-dextran (D) was further evaluated in the present study as a lymphoscintigraphic agent compared to radiocolloids. It was injected intra-dermally into the web space between the second and third toes in both hind feet of two rabbits. 99mTc-nanocolloid (NC) was injected subcutaneously in both hind feet of two other rabbits. Popliteal lymph nodes were taken out and frozen in liquid nitrogen after the animals were sacrificed at 2 h post-injection. Three frozen sections in 10 microns thickness were prepared from each node for autoradiographic studies. The lymph node slices were exposed for 18 h using Ilford G 5 emulsion. The obtained autoradiographs showed that the distribution of 99mTc-D radioactivity within lymph nodes was more uniform indicating better tissue penetration compared to 99mTc-NC which remained mostly in the lymph canaliculi.
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Abteilung für Nuklearbiologie der Gesellschaft fäur Strahlen-und Unweltforschung mbH, München, Fed. Rep., Germany.
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D000818:Animals; D001345:Autoradiography; D003102:Colloids; D003911:Dextrans; D008198:Lymph Nodes / Q000000981:diagnostic imaging*; D015609:Organotechnetium Compounds; D011817:Rabbits; D011877:Radionuclide Imaging
D003102:Colloids; D003911:Dextrans; D015609:Organotechnetium Compounds; C034146:technetium Tc 99m dextran