An endothelial ligand for L-selectin is a novel mucin-like molecule.
The adhesive interaction between circulating lymphocytes and the high endothelial venules (HEV) of lymph nodes (LN) is mediated by lymphocyte L-selectin, a member of the selectin family of cell adhesion proteins. Previous work has identified a sulfated 50 kd glycoprotein (Sgp50) as an HEV ligand for L-selectin. We now report the purification of this glycoprotein and the utilization of the derived N-terminal amino acid sequence to clone a cDNA. The predicted sequence reveals a novel, mucin-like molecule containing two serine/threonine-rich domains. The mRNA encoding this glycoprotein is preferentially expressed in LN. Antibodies against predicted peptides immunoprecipitate Sgp50 and stain the apical surface of LN HEV. These results thus define a tissue-specific mucin-like endothelial glycoprotein that appears to function as a scaffold that presents carbohydrates to the L-selectin lectin domain.
Lasky
L A
LA
Department of Immunobiology, Genentech, Inc,, South San Francisco, California 94080.
Singer
M S
MS
Dowbenko
D
D
Imai
Y
Y
Henzel
W J
WJ
Grimley
C
C
Fennie
C
C
Gillett
N
N
Watson
S R
SR
Rosen
S D
SD
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D005796:Genes; D019041:L-Selectin; D008024:Ligands; D051379:Mice; D008969:Molecular Sequence Data; D009077:Mucins / Q000235:genetics* / Q000378:metabolism*; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D011487:Protein Conformation
D015815:Cell Adhesion Molecules; D008024:Ligands; D009077:Mucins; D009838:Oligodeoxyribonucleotides; D019041:L-Selectin; C075185:sulfated glycoprotein p50; D004247:DNA