title: Localization of a 215-kDa tyrosine-phosphorylated protein that cross-reacts with tensin antibodies.
abstract: Tyrosine phosphorylation of cytoskeletal proteins at adhesive junctions has been speculated to play a role in the regulation of cell signaling at these sites. Previously, monoclonal antibodies were generated against phosphotyrosine-containing proteins from Rous sarcoma virus-transformed chick embryo fibroblasts, resulting in two antibodies which recognized antigens of 76 and 215 kDa that localized to focal contacts. We have now localized the 215-kDa antigen to a number of adhesive junctions in vivo, including the zonula adherens, intercalated discs, and myotendinous and neuromuscular junctions. In sections of skeletal muscle and in isolated myofibrils, the 215-kDa protein was localized to the I-band. By immunoprecipitation and immunoblot analysis, we determined that the 215-kDa antigen cross-reacts with a polyclonal anti-tensin antibody.
authors.affiliation: Department of Cell Biology and Anatomy, University of North Carolina, Chapel Hill 27599-7090.
authors.forename: S M
authors.initials: SM
authors.lastname: Bockholt
authors.forename: C A
authors.initials: CA
authors.lastname: Otey
authors.forename: J R
authors.initials: JR
authors.lastname: Glenney
authors.forename: K
authors.initials: K
authors.lastname: Burridge
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D001358:Avian Sarcoma Viruses / Q000235:genetics; D015815:Cell Adhesion Molecules / Q000032:analysis* / Q000276:immunology; D002642:Chick Embryo; D003429:Cross Reactions; D004847:Epithelial Cells; D005347:Fibroblasts / Q000166:cytology; D005455:Fluorescent Antibody Technique; D015151:Immunoblotting; D007365:Intercellular Junctions / Q000648:ultrastructure*; D007908:Lens, Crystalline / Q000166:cytology; D008840:Microfilament Proteins / Q000032:analysis* / Q000276:immunology; D008970:Molecular Weight; D009130:Muscle, Smooth / Q000166:cytology; D009132:Muscles / Q000166:cytology; D009206:Myocardium / Q000166:cytology; D009210:Myofibrils / Q000648:ultrastructure; D010750:Phosphoproteins / Q000032:analysis*; D010766:Phosphorylation; D019000:Phosphotyrosine; D051381:Rats; D000070876:Tensins; D014443:Tyrosine / Q000031:analogs & derivatives / Q000032:analysis
chemical_list: D000911:Antibodies, Monoclonal; D015815:Cell Adhesion Molecules; D008840:Microfilament Proteins; D010750:Phosphoproteins; D000070876:Tensins; C000603372:Tns1 protein, rat; D019000:Phosphotyrosine; D014443:Tyrosine