title: Identification and endothelin-induced activation of multiple extracellular signal-regulated kinases in aortic smooth muscle cells.
abstract: In order to explore intracellular signaling pathways of the mitogenic action of endothelin (ET), we examined the effect of ET on activities of extracellular signal-regulated kinases (ERKs) in rat aortic smooth muscle cells (SMCs). Treatment of rat aortic SMCs with ET-1 increased kinase activities toward myelin basic protein (MBP). Both 43- and 41-kDa proteins were activated when kinase assays were done in MBP-containing polyacrylamide gels after SDS-PAGE. These proteins were identified as ERK1 and ERK2 with immunoprecipitation and immunoblotting using antipeptide antibodies, respectively. These results indicate that ERKs mediate signal transduction by ET.
authors.affiliation: Department of Medicine, Kyoto University School of Medicine, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Yoshimasa
authors.forename: K
authors.initials: K
authors.lastname: Nakao
authors.forename: S
authors.initials: S
authors.lastname: Suga
authors.forename: I
authors.initials: I
authors.lastname: Kishimoto
authors.forename: Y
authors.initials: Y
authors.lastname: Kiso
authors.forename: H
authors.initials: H
authors.lastname: Imura
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001013:Aorta, Thoracic / Q000187:drug effects / Q000201:enzymology*; D015153:Blotting, Western; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D016232:Endothelins / Q000494:pharmacology*; D007106:Immune Sera; D007700:Kinetics; D008969:Molecular Sequence Data; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000201:enzymology*; D004676:Myelin Basic Protein / Q000378:metabolism*; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology; D011505:Protein-Tyrosine Kinases / Q000302:isolation & purification / Q000378:metabolism*; D011506:Proteins / Q000302:isolation & purification / Q000378:metabolism*; D051381:Rats; D015398:Signal Transduction
chemical_list: D016232:Endothelins; D007106:Immune Sera; D004676:Myelin Basic Protein; D010455:Peptides; D011506:Proteins; D011505:Protein-Tyrosine Kinases; D017871:Calcium-Calmodulin-Dependent Protein Kinases