Hemostatic defects associated with two infusion rates of dextran 70 in dogs.
We investigated changes in hemostatic function after infusion of 6% dextran 70 (high molecular weight dextran) at 2 rates. Six healthy dogs underwent 3 regimens: 20 ml of dextran/kg of body weight administered in 1 hour (trial A), 20 ml of dextran/kg administered in 30 minutes (trial B), and 0.9% sodium chloride solution as a control administered over 1 hour to achieve hemodilution equivalent to that for 20 ml of dextran/kg (trial C). Before and at 2, 4, 8, and 24 hours after the start of trials A and B, we measured PCV, total solids (TS) concentration, amount of von Willebrand factor antigen (vWf:Ag), factor VIII coagulant activity (VIII:C), prothrombin time, activated partial thromboplastin time (APTT), platelet retention in a glass bead column, and buccal mucosa bleeding time (BMBT). Values were not obtained at 8 and 24 hours for trial C. Saline-induced changes in hemostasis were significant (P less than 0.05) from baseline throughout the sample collection period. Significant differences (P less than 0.05) between trial A and control were observed for vWf:Ag, VIII:C, BMBT, APTT, TS, and PCV values at 2 hours, and for VIII:C at 4 hours. Significant differences (P less than 0.05) between trial B and control were observed for APTT, TS, and PCV values at 2 hours, and for vWf:Ag, VIII:C, BMBT, APTT, TS, and PCV values at 4 hours. During trials A and B, mean values of analytes infrequently deviated from reference intervals, and clinical signs of bleeding were not observed in any dog.(ABSTRACT TRUNCATED AT 250 WORDS)
Concannon
K T
KT
Veterinary Medical Teaching Hospital, School of Veterinary Medicine, University of California, Davis 95616.
Haskins
S C
SC
Feldman
B F
BF
D000818:Animals; D001760:Bleeding Time / Q000662:veterinary; D003911:Dextrans / Q000008:administration & dosage / Q000494:pharmacology*; D004285:Dogs / Q000502:physiology*; D005169:Factor VIII / Q000032:analysis; D005260:Female; D006400:Hematocrit / Q000662:veterinary; D006487:Hemostasis / Q000187:drug effects*; D007262:Infusions, Intravenous / Q000662:veterinary; D008297:Male; D010314:Partial Thromboplastin Time / Q000662:veterinary; D011517:Prothrombin Time / Q000662:veterinary; D014841:von Willebrand Factor / Q000032:analysis
D003911:Dextrans; D014841:von Willebrand Factor; D005169:Factor VIII