Simultaneous determination of (R)- and (S)-naproxen and (R)- and (S)-6-O-desmethylnaproxen by high-performance liquid chromatography on a Chiral-AGP column.
A high-performance liquid chromatographic method for the simultaneous determination of both enantiomers of naproxen and its metabolite 6-O-desmethylnaproxen has been developed. The separation is performed on a column containing alpha 1-acid glycoprotein as the chiral selector. The method has been used for the determination of the enantiomeric purity of the drug substance and the metabolite, and for the simultaneous determination of all four compounds in biological fluids.
Andersen
J V
JV
PharmaBiotec Research Center, Royal Danish School of Pharmacy, Department of Organic Chemistry, Copenhagen.
Hansen
S H
SH
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D005260:Female; D009288:Naproxen / Q000031:analogs & derivatives* / Q000652:urine*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013050:Spectrometry, Fluorescence; D013237:Stereoisomerism
D009288:Naproxen; C034451:desmethylnaproxen