Expression, molecular association, and functions of C3 complement receptors CR1 (CD35) and CR2 (CD21) on the human T cell line HPB-ALL.
We have investigated the expression, molecular association, ligand binding properties, and ability to transduce intracellular signals of CR1 and CR2 C3 receptors on cells of the human HPB-ALL T cell line. CR1 and CR2 on HPB-ALL cells bound polymeric C3b and C3dg and several anti-CR1 and anti-CR2 mAb recognizing different epitopes of the receptors on normal peripheral blood cells. Immunoprecipitated CR1 and CR2 exhibited similar m.w. to those of the receptors on normal peripheral blood T and B lymphocytes. CR1 and CR2 were partially associated in the form of CR1/CR2 complexes in the cell membrane as assessed by the ability of the receptors to cocap and cointernalize and to form a detergent-sensitive complex upon immunoprecipitation analysis. Triggering of CR2 with mAb OKB7 that recognizes an epitope associated with the ligand binding site of the receptor induced an increase in intracellular free calcium concentration in HPB-ALL cells. The signal provided by mAb OKB7 did not synergize with that triggered by anti-CD3 mAb UCHT1. Triggering of CR1 did not result in changes in intracellular free calcium concentration. Our observations have significance for the biology of normal human T cells because the majority of peripheral blood T cells that express CR1 also expressed CR2 and because a change in (Ca2+)i was induced by mAb OKB7 in purified normal T cells. These functions may be relevant for the regulatory role of C3 fragments on the immune response to T-dependent Ag and for the penetration into T cells of lymphocytotropic viruses.
Delibrias
C C
CC
INSERM U 28, Hopital Broussais, Paris, France.
Fischer
E
E
Bismuth
G
G
Kazatchkine
M D
MD
D000944:Antigens, Differentiation, B-Lymphocyte / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D002460:Cell Line; D004705:Endocytosis; D005434:Flow Cytometry; D006801:Humans; D007152:Immunologic Capping; D066298:In Vitro Techniques; D008024:Ligands; D011940:Receptor Aggregation; D011951:Receptors, Complement / Q000378:metabolism*; D017463:Receptors, Complement 3b; D017464:Receptors, Complement 3d; D012397:Rosette Formation; D015398:Signal Transduction; D013601:T-Lymphocytes / Q000378:metabolism*
D000944:Antigens, Differentiation, B-Lymphocyte; D008024:Ligands; D011951:Receptors, Complement; D017463:Receptors, Complement 3b; D017464:Receptors, Complement 3d; D002118:Calcium