Endocrine cells and nerves in the pyloric ceca and the intestine of Oncorhynchus mykiss (Teleostei): an immunocytochemical study.
The endocrine cells of rainbow trout pyloric ceca and intestine have been investigated immunocytochemically using the avidin-biotin method. Twenty-six antisera were tested and 13 endocrine cell types immunoreacted with antisera to serotonin, somatostatin-25, bombesin, C-flanking bombesin, substance P, salmon PP, NPY, PYY, PP, glucagon, GLP1, Met-enkephalin, and CCK/G. Glucagon and GLP1 immunoreactivities appear in the same cells. Nerves positive to serotonin, substance P, PHI, and VIP were also found. The presence of cells positive to somatostatin-25, C-flanking bombesin, and salmon PP are described for the first time in fish intestine.
Beorlegui
C
C
Department of Histology and Pathology, University of Navarra, Pamplona, Spain.
Martínez
A
A
Sesma
P
P
D000818:Animals; D001839:Bombesin / Q000378:metabolism; D002432:Cecum / Q000166:cytology* / Q000294:innervation; D002766:Cholecystokinin / Q000378:metabolism; D004702:Endocrine Glands / Q000166:cytology* / Q000294:innervation; D004745:Enkephalins / Q000378:metabolism; D005755:Gastrins / Q000378:metabolism; D005934:Glucagon / Q000378:metabolism; D007150:Immunohistochemistry; D010191:Pancreatic Polypeptide / Q000378:metabolism; D012474:Salmon / Q000502:physiology*; D012701:Serotonin / Q000502:physiology; D016622:Silver Staining; D013004:Somatostatin / Q000502:physiology; D013373:Substance P / Q000378:metabolism; D014337:Trout / Q000502:physiology*
D004745:Enkephalins; D005755:Gastrins; D012701:Serotonin; D013373:Substance P; D013004:Somatostatin; D010191:Pancreatic Polypeptide; D005934:Glucagon; D002766:Cholecystokinin; D001839:Bombesin