Ribosomes from trichomonad protozoa have prokaryotic characteristics.
1. Ribosomes from cells of the genera Trichomonas and Tritrichomonas have been isolated and characterized. The ribosomes from each organism had a sedimentation coefficient of 70S in calibrated sucrose gradients and the subunits sedimented as 50S and 30S particles under the same conditions. 2. The major ribosomal RNAs from each species were identical in size to prokaryotic ribosomal RNAs when examined by denaturing gel electrophoresis. The ribosomes contained both 5.8S and 5S RNAs. 3. The ribosomal proteins were compared by the methods of two-dimensional gel electrophoresis and reversed phase HPLC. Electrophoresis of the ribosomal proteins in two different gel systems indicated the presence of 56 proteins in T. gallinae, 40 in T. bactrachorum and 45 in the Tritrichomonas sp. The protein molecular mass range was 8.5-40 kDa. 4. The HPLC analysis confirmed the protein number established by the gel methods. 5. Both methods of analysis revealed greater similarities between the ribosomal proteins of the 2 Tritrichomonas sp. than between those of the more distantly related T. gallinae and T. bactrachorum.
Champney
W S
WS
Department of Biochemistry, East Tennessee State University, Johnson City 37614.
Chittum
H S
HS
Samuels
R
R
D000818:Animals; D002499:Centrifugation, Density Gradient; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D008970:Molecular Weight; D011387:Prokaryotic Cells / Q000648:ultrastructure; D012335:RNA, Ribosomal / Q000302:isolation & purification; D012269:Ribosomal Proteins / Q000302:isolation & purification; D012270:Ribosomes / Q000737:chemistry*; D014244:Trichomonas / Q000648:ultrastructure*; D014318:Tritrichomonas / Q000648:ultrastructure*
D012335:RNA, Ribosomal; D012269:Ribosomal Proteins