High-performance liquid chromatographic determination of sulphobromophthalein and its conjugates.
A simple, sensitive and selective high-performance liquid chromatographic method for the determination of sulphobromophthalein and its mercaptide conjugates in rat bile was developed. These pigments, which have an absorption maximum at 580 nm in alkaline solution, were separated isocratically on an alkali-resistant ODS column by paired-ion chromatography. Analysis of bile samples obtained after intravenous administration of sulphobromophthalein to rats showed the presence of at least twenty peaks of metabolites, of which thirteen were identified and seven quantified.
Sano
K
K
Department of Biopharmacy, Meiji College of Pharmacy, Tokyo, Japan.
Kinosita
I
I
Mihara
R
R
Ikegami
Y
Y
Uesugi
T
T
D000818:Animals; D001646:Bile / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002855:Chromatography, Thin Layer; D003545:Cysteine / Q000378:metabolism; D004151:Dipeptides / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007536:Isomerism; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013438:Sulfhydryl Compounds / Q000032:analysis*; D013448:Sulfobromophthalein / Q000032:analysis* / Q000378:metabolism
D004151:Dipeptides; D013438:Sulfhydryl Compounds; D013448:Sulfobromophthalein; C028505:cysteinylglycine; D005978:Glutathione; D003545:Cysteine