title: Prolonged hypoxia induced by the reduction of maternal uterine blood flow alters insulin-like growth factor-binding protein-1 (IGFBP-1) and IGFBP-2 gene expression in the ovine fetus.
abstract: Insulin-like growth factors (IGF-I and IGF-II) are potent mitogenic and differentiating peptides which are synthesized by many fetal tissues. In the circulation and tissue fluids, IGFs are bound to binding proteins (BPs) which not only function as carrier proteins, but also inhibit or modulate the biological actions of IGFs. We have previously shown that prolonged hypoxia in the ovine fetus induced by the reduction of maternal uterine blood flow for 24 h causes a reduction in the DNA synthesis rate in selected fetal tissues. To determine if this effect is due to alterations in the local synthesis of tissue IGFs and their binding proteins or to changes in systemic concentrations of IGFs and IGFBPs, we have investigated the abundance of mRNAs encoding IGFs and IGFBPs in selected tissues and changes in plasma IGFs and IGFBPs. Ovine fetuses (115-120 days gestation; n = 6) underwent 24 h of hypoxia by the reduction of maternal uterine blood flow (RUBF). Controls (n = 6) underwent the same surgical procedure without RUBF. Serial plasma samples were collected before, during, and after the experiment, and tissues were collected at the end of 24 h. Mean plasma IGF-I and IGF-II concentrations tended to be lower in hypoxic fetuses than in controls during the course of hypoxia, but these differences were not statistically significant. Tissue mRNA levels for IGF-I and IGF-II in lung, muscle, thymus, and kidney were similar in control and hypoxic fetuses after 24 h of hypoxia. The relative abundance of liver IGF-I and IGF-II mRNAs was lower in hypoxic fetuses, but only IGF-I mRNA levels were significantly different from the control values (P < 0.05). Compared to control fetuses, IGFBP-1 mRNA levels in the liver of hypoxic fetuses were increased 3- to 7-fold, and IGFBP-1 mRNA expression was induced in kidneys of some hypoxic fetuses (two of six). In addition, IGFBP-2 mRNA levels were decreased in the liver (50%) and kidney (30%) of hypoxic fetuses. The increase in liver IGFBP-1 mRNA abundance and the decrease in liver and kidney IGFBP-2 mRNA abundance were accompanied by an increase in IGFBP-1 levels and a decrease in IGFBP-2 levels in fetal plasma. No changes were observed in either plasma levels or tissue mRNA abundance for IGFBP-3. Analysis of the time course of changes in plasma revealed that the changes in IGFBP-1 and IGFBP-2 occurred within 4 h of hypoxia.(ABSTRACT TRUNCATED AT 400 WORDS)
authors.affiliation: Medical Research Council Group in Fetal and Neonatal Health and Development, University of Western Ontario, Lawson Research Institute, London, Canada.
authors.forename: K C
authors.initials: KC
authors.lastname: McLellan
authors.forename: S B
authors.initials: SB
authors.lastname: Hooper
authors.forename: A D
authors.initials: AD
authors.lastname: Bocking
authors.forename: P J
authors.initials: PJ
authors.lastname: Delhanty
authors.forename: I D
authors.initials: ID
authors.lastname: Phillips
authors.forename: D J
authors.initials: DJ
authors.lastname: Hill
authors.forename: V K
authors.initials: VK
authors.lastname: Han
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000097:blood / Q000235:genetics*; D005260:Female; D005312:Fetal Blood; D005315:Fetal Diseases / Q000235:genetics*; D005333:Fetus / Q000378:metabolism / Q000502:physiology*; D015870:Gene Expression / Q000502:physiology*; D000860:Hypoxia / Q000235:genetics*; D018970:Insulin-Like Growth Factor Binding Protein 1; D018971:Insulin-Like Growth Factor Binding Protein 2; D011247:Pregnancy; D012333:RNA, Messenger / Q000378:metabolism; D012039:Regional Blood Flow; D012756:Sheep; D013002:Somatomedins / Q000235:genetics; D014599:Uterus / Q000098:blood supply*
chemical_list: D002352:Carrier Proteins; D018970:Insulin-Like Growth Factor Binding Protein 1; D018971:Insulin-Like Growth Factor Binding Protein 2; D012333:RNA, Messenger; D013002:Somatomedins