[Effect of toxic components of snake venoms on binding of substance P with rat brain membranes].
Ion-exchange HPLC is used for purification of the snake venom alpha-neurotoxins, chi-bungarotoxin, cytotoxins, and phospholipases A2. Among these purified polypeptides, phospholipases A2 are found to be the most potent in inhibiting the substance P binding to rat brain membranes, Ki approximately 10(-8) M. Other toxins are weak inhibitors (Ki greater than or equal to 10(-4)-10(-5) M), earlier data on the inhibiting activity of alpha-bungarotoxin being caused by the commercial preparations' contamination with phospholipase A2.
Utkin
Iu N
IuN
Kasheverov
I E
IE
Tsetlin
V I
VI
D000818:Animals; D001921:Brain / Q000378:metabolism*; D002038:Bungarotoxins / Q000302:isolation & purification; D002462:Cell Membrane / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D003603:Cytotoxins / Q000302:isolation & purification; D009498:Neurotoxins / Q000302:isolation & purification; D010741:Phospholipases A / Q000302:isolation & purification; D054467:Phospholipases A2; D051381:Rats; D012910:Snake Venoms / Q000737:chemistry*; D013373:Substance P / Q000378:metabolism*
D002038:Bungarotoxins; D003603:Cytotoxins; D009498:Neurotoxins; D012910:Snake Venoms; D013373:Substance P; D010741:Phospholipases A; D054467:Phospholipases A2