Differential regulation of manganese and copper/zinc superoxide dismutases by the facial nerve transection.
The effects of unilateral nerve transection on manganese and copper/zinc superoxide dismutase (Mn-SOD and Cu/Zn-SOD) mRNA levels in the facial nucleus were studied by in situ hybridization. An increase of Mn-SOD mRNA levels was first seen in the ipsilateral facial nucleus 12 h after axotomy, and was most pronounced at 4-7 days after this procedure; by 56 days, the increase disappeared. There was no change in Cu/Zn-SOD mRNA levels at any time after axotomy. We further confirmed, by immunohistochemistry, that the increase in Mn-SOD transcription was followed by protein synthesis. These results are suggestive of an important role for Mn-SOD in defense, regeneration and recovery responses following nerve transection.
Yoneda
T
T
First Department of Anatomy, Osaka City University Medical School, Japan.
Inagaki
S
S
Hayashi
Y
Y
Nomura
T
T
Takagi
H
H
D000818:Animals; D005154:Facial Nerve / Q000502:physiology*; D007150:Immunohistochemistry; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism*
D013482:Superoxide Dismutase