title: Crystal structure of the phosphotyrosine recognition domain SH2 of v-src complexed with tyrosine-phosphorylated peptides.
abstract: Three-dimensional structures of complexes of the SH2 domain of the v-src oncogene product with two phosphotyrosyl peptides have been determined by X-ray crystallography at resolutions of 1.5 and 2.0 A, respectively. A central antiparallel beta-sheet in the structure is flanked by two alpha-helices, with peptide binding mediated by the sheet, intervening loops and one of the helices. The specific recognition of phosphotyrosine involves amino-aromatic interactions between lysine and arginine side chains and the ring system in addition to hydrogen-bonding interactions with the phosphate.
authors.affiliation: Rockefeller University, New York.
authors.forename: G
authors.initials: G
authors.lastname: Waksman
authors.forename: D
authors.initials: D
authors.lastname: Kominos
authors.forename: S C
authors.initials: SC
authors.lastname: Robertson
authors.forename: N
authors.initials: N
authors.lastname: Pant
authors.forename: D
authors.initials: D
authors.lastname: Baltimore
authors.forename: R B
authors.initials: RB
authors.lastname: Birge
authors.forename: D
authors.initials: D
authors.lastname: Cowburn
authors.forename: H
authors.initials: H
authors.lastname: Hanafusa
authors.forename: B J
authors.initials: BJ
authors.lastname: Mayer
authors.forename: M
authors.initials: M
authors.lastname: Overduin
authors.forename: M D
authors.initials: MD
authors.lastname: Resh
authors.forename: C B
authors.initials: CB
authors.lastname: Rios
authors.forename: L
authors.initials: L
authors.lastname: Silverman
authors.forename: J
authors.initials: J
authors.lastname: Kuriyan
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D003461:Crystallography; D046911:Macromolecular Substances; D008958:Models, Molecular; D008969:Molecular Sequence Data; D015688:Oncogene Protein pp60(v-src) / Q000378:metabolism / Q000648:ultrastructure*; D010455:Peptides / Q000378:metabolism; D019000:Phosphotyrosine; D011485:Protein Binding; D011487:Protein Conformation; D011505:Protein-Tyrosine Kinases / Q000378:metabolism / Q000648:ultrastructure*; D016415:Sequence Alignment; D015398:Signal Transduction; D012997:Solvents; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000378:metabolism; D014961:X-Ray Diffraction
chemical_list: D046911:Macromolecular Substances; D010455:Peptides; D012997:Solvents; D019000:Phosphotyrosine; D014443:Tyrosine; D011505:Protein-Tyrosine Kinases; D015688:Oncogene Protein pp60(v-src)