Enolase is present at the centrosome of HeLa cells.
Antibodies raised against the C-terminus and N-terminus region of gamma gamma enolase, as well as a polyclonal antibody raised against bovine brain gamma gamma enolase, were used to study the distribution of this glycolytic enzyme during the cell cycle in HeLa cells. Enolase was found to be present throughout the cytoplasm of both interphase and dividing cells. In addition, a portion of cellular enolase was detected at the centrosome throughout the cell cycle. The capacity of glycolytic enzymes to play a structural as well as a glycolytic role suggests that the presence of enolase at the centrosome may be correlated with the organization of both the interphase cytoskeleton and the mitotic spindle.
Johnstone
S A
SA
Department of Medical Biochemistry, University of Calgary, Alberta, Canada.
Waisman
D M
DM
Rattner
J B
JB
D000595:Amino Acid Sequence; D015153:Blotting, Western; D002455:Cell Division; D002503:Centromere / Q000201:enzymology*; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D010751:Phosphopyruvate Hydratase / Q000032:analysis*
D010751:Phosphopyruvate Hydratase