B-50/GAP43 localization on membranes of putative transport vesicles in the cell body, neurites and growth cones of cultured hippocampal neurons.
We conducted an electron microscopic immunocytochemical study to locate B-50/GAP43 in various cellular elements of hippocampal neurons grown in dissociated cell culture. B-50 was detected with pre- and post-embedding immunoincubation, using affinity-purified B-50 antibodies and secondary antibodies coated on gold probes. For the first time ultrastructural evidence is presented for the location of B-50 on the membranes of electron-lucent transport vesicles with a diameter of 99.4 +/- 2.9 nm, present in the trans region of the Golgi apparatus, in neurites and in growth cones of cultured hippocampal neurons.
Van Lookeren Campagne
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Rudolf Magnus Institute, University of Utrecht, Netherlands.
Dotti
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Verkleij
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Gispen
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Oestreicher
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D000818:Animals; D001369:Axons / Q000737:chemistry / Q000648:ultrastructure; D001692:Biological Transport; D002478:Cells, Cultured; D019922:GAP-43 Protein; D006056:Golgi Apparatus / Q000737:chemistry* / Q000648:ultrastructure; D006624:Hippocampus / Q000166:cytology* / Q000196:embryology / Q000378:metabolism; D007150:Immunohistochemistry; D007425:Intracellular Membranes / Q000737:chemistry*; D008562:Membrane Glycoproteins / Q000032:analysis*; D016253:Microscopy, Immunoelectron / Q000379:methods; D009419:Nerve Tissue Proteins / Q000032:analysis*; D016501:Neurites / Q000737:chemistry / Q000648:ultrastructure; D009474:Neurons / Q000737:chemistry* / Q000648:ultrastructure; D015388:Organelles / Q000737:chemistry / Q000648:ultrastructure; D051381:Rats
D019922:GAP-43 Protein; D008562:Membrane Glycoproteins; D009419:Nerve Tissue Proteins