title: DNA adducts in carp exposed to artificial diesel-2 oil slicks.
abstract: In attempts to mimic field exposure, oil slicks prepared from diesel-2 oil/water emulsions were poured onto the surface of water in tanks prepared fresh every day and liver DNA adducts were analyzed by 32P-postlabeling in carp free-swimming in these tanks. 'Clusters' of lipophilic DNA adducts were detected, with five major and numerous minor adducts. Essentially a similar adduct pattern was found in the liver DNA of carp exposed to crude oil-polluted water. Diesel-2 adduct induction was observed slowly with a steady increase to greater than 3000 amol/microgram DNA at day 12. After this time fish were transferred to clean water. Adduct levels continued to increase through day 17 (approximately 10,000 amol/microgram DNA) despite the cessation of exposure, but a 30% and 80% decline was evident at day 22 and day 27, respectively. All major adducts were distinct from the known benzo[a]pyrene diolepoxide-dG. These results indicate that diesel-2 oil can cause extensive DNA damage in carp in vivo and the damage accumulates proportionately with time of exposure.
authors.affiliation: Center for Marine Research Zagreb, Ruder Boskovic Institute, Croatia.
authors.forename: B
authors.initials: B
authors.lastname: Kurelec
authors.forename: A
authors.initials: A
authors.lastname: Garg
authors.forename: S
authors.initials: S
authors.lastname: Krca
authors.forename: S
authors.initials: S
authors.lastname: Britvić
authors.forename: D
authors.initials: D
authors.lastname: Lucić
authors.forename: R C
authors.initials: RC
authors.lastname: Gupta
mesh_terms: D000818:Animals; D002347:Carps / Q000235:genetics*; D004247:DNA / Q000302:isolation & purification; D004249:DNA Damage; D004785:Environmental Pollutants / Q000633:toxicity; D005742:Gasoline / Q000633:toxicity*; D008099:Liver / Q000737:chemistry; D010578:Petroleum / Q000633:toxicity; D010761:Phosphorus Radioisotopes
chemical_list: D004785:Environmental Pollutants; D005742:Gasoline; D010578:Petroleum; D010761:Phosphorus Radioisotopes; D004247:DNA