title: Synthesis of hydrolytic enzymes during production of tylosin by Streptomyces fradiae.
abstract: The exposure of a wild-type tylosin producing strain of Streptomyces fradiae to mutagenic agents resulted in the isolation of several tylosin over-producing strains. Examination of three mutants, T4310, 612 and 3204 showed that improved tylosin production was associated with increased hydrolytic enzyme activity and cell growth. The wild-type strain showed lower levels of hydrolytic activity including, protease, amylase, lipase and esterase activities and attained a lower cell density than the mutants.
authors.affiliation: Development Center for Biotechnology, Taipei, Taiwan, ROC.
authors.forename: M C
authors.initials: MC
authors.lastname: Brahimi-Horn
authors.forename: S H
authors.initials: SH
authors.lastname: Luo
authors.forename: S L
authors.initials: SL
authors.lastname: Wang
authors.forename: S W
authors.initials: SW
authors.lastname: Gau
authors.forename: D G
authors.initials: DG
authors.lastname: Mou
mesh_terms: D000681:Amylases / Q000378:metabolism; D010450:Endopeptidases / Q000378:metabolism; D004950:Esterases / Q000378:metabolism; D005285:Fermentation; D007700:Kinetics; D008049:Lipase / Q000378:metabolism; D009154:Mutation; D013302:Streptomyces / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D015645:Tylosin / Q000096:biosynthesis*
chemical_list: D004950:Esterases; D008049:Lipase; D000681:Amylases; D010450:Endopeptidases; D015645:Tylosin