Phenotypes conferred by the Bacillus subtilis recM13 mutation and the din23 fusion.
The din23 fusion encodes a B. subtilis SOS-inducible regulatory region fused to the E. coli lacZ gene (Love et al., 1985). A strain encoding the din23 fusion and a recM13 allele showed low-level constitutive beta-galactosidase expression, was induced for beta-galactosidase production by DNA gyrase inhibitors but not by DNA-damaging agents, and was slightly induced by a variety of agents which do not normally induce the SOS regulon. The din23 fusion itself resulted in high levels of spontaneous prophage induction in wild-type, recM- and recA-hosts, despite the fact that the din23recM13 strain was not induced for beta-galactosidase production by DNA-damaging agents. The results suggest that the recM gene may be involved with the regulation of the RecA protease-mediated SOS response, while the din23 gene may be involved with the regulation of an alternative function which results in the cleavage of prophage repressor.
Osburne
M S
MS
American Cyanamid Company, Medical Research Division, Lederle Laboratories, Pearl River, NY 10965.
Zavodny
S M
SM
Greenstein
M
M
Maiese
W M
WM
D001412:Bacillus subtilis / Q000235:genetics*; D001435:Bacteriophages / Q000235:genetics; D004274:DNA, Recombinant; D005798:Genes, Bacterial; D005809:Genes, Regulator; D008242:Lysogeny; D009154:Mutation; D013014:SOS Response, Genetics; D014779:Virus Replication; D001616:beta-Galactosidase / Q000235:genetics
D004274:DNA, Recombinant; D001616:beta-Galactosidase