title: The beneficial effect of dextran on anastomotic patency and flap survival in a strongly thrombogenic model.
abstract: Dextran lowers the probability of thrombus formation, by reducing platelet aggregation and adhesion and by increasing fibrinolysis. All studies to date using dextran for microvascular reconstruction have examined only short-term (1 to 2 hr) patency in isolated vessels. The current study used an established thrombotic model (inverted sleeve interposition graft), to investigate the effect of dextran on the long-term survival of pedicle flaps. A6- x 3-cm epigastric flap was elevated. A 2-mm inverted sleeve interposition graft was placed on the artery side of the pedicle by microvascular techniques. One group received dextran (17 cc/kg) 2 hr preoperatively and every 6 hr postoperatively for the next 72 hr. A control group received saline on the same schedule. Survival was assessed on postoperative day 7, at which time necrosis was obvious. Several parameters (clotting studies and electron microscopy) were studied to characterize the phenomenon more clearly. Only 25 percent of saline-treated flaps survived (2/8), while all dextran-treated flaps survived (7/7) (p less than 0.02, Fisher exact test). Thus, dextran allowed flaps to survive by preventing thrombus formation, despite a strong thrombogenic focus.
authors.affiliation: Department of Plastic Surgery, University of Virginia Health Sciences Center, Charlottesville.
authors.forename: S F
authors.initials: SF
authors.lastname: Wolfort
authors.forename: M F
authors.initials: MF
authors.lastname: Angel
authors.forename: K R
authors.initials: KR
authors.lastname: Knight
authors.forename: L R
authors.initials: LR
authors.lastname: Amiss
authors.forename: R F
authors.initials: RF
authors.lastname: Morgan
mesh_terms: D000818:Animals; D003911:Dextrans / Q000627:therapeutic use*; D005263:Femoral Artery / Q000648:ultrastructure; D006083:Graft Occlusion, Vascular / Q000517:prevention & control*; D006085:Graft Survival / Q000187:drug effects*; D008855:Microscopy, Electron, Scanning; D011817:Rabbits; D012965:Sodium Chloride / Q000627:therapeutic use; D013524:Surgical Flaps / Q000502:physiology*; D013927:Thrombosis / Q000517:prevention & control*; D013997:Time Factors; D014654:Vascular Patency / Q000187:drug effects*
chemical_list: D003911:Dextrans; D012965:Sodium Chloride