Recombinant human stem cell factor stimulates growth of a human glioblastoma cell line expressing c-kit protooncogene.
The growth of a panel of eight different human glioblastoma cell lines was examined in a human tumor cloning assay in agar, a tritiated thymidine uptake assay, and by counting cell numbers, in cultures performed in the absence or presence of increasing concentrations (1 to 100 ng/ml) of recombinant human stem cell factor (SCF). Growth of 7 of 8 cell lines was not significantly and reproducibly affected by recombinant human SCF. However, growth of the CRL 1620 cell line could be stimulated up to 5-fold by the cytokine. In contrast to the other cell lines investigated, CRL 1620 expressed the c-kit protooncogene assessed on the mRNA and protein level. Furthermore, SCF-induced proliferation of CRL 1620 cells was sensitive to the tyrosine kinase inhibitor erbstatin. Our data suggest that SCF can be operative in growth modulation of malignant cells outside the hematopoietic system, and this finding should be further studied for its possible clinical implications.
Berdel
W E
WE
Department of Medicine/Hematology and Oncology, Klinikum Steglitz, Freie Universitaet Berlin, Germany.
de Vos
S
S
Maurer
J
J
Oberberg
D
D
von Marschall
Z
Z
Schroeder
J K
JK
Li
J
J
Ludwig
W D
WD
Kreuser
E D
ED
Thiel
E
E
D002455:Cell Division / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry; D005910:Glioma / Q000235:genetics / Q000473:pathology*; D016298:Hematopoietic Cell Growth Factors / Q000494:pharmacology*; D006801:Humans; D011518:Proto-Oncogene Proteins / Q000032:analysis; D019009:Proto-Oncogene Proteins c-kit; D011519:Proto-Oncogenes; D011994:Recombinant Proteins / Q000494:pharmacology; D019089:Stem Cell Factor; D014407:Tumor Cells, Cultured; D014410:Tumor Stem Cell Assay
D016298:Hematopoietic Cell Growth Factors; D011518:Proto-Oncogene Proteins; D011994:Recombinant Proteins; D019089:Stem Cell Factor; D019009:Proto-Oncogene Proteins c-kit