title: Excretion of formaldehyde, malondialdehyde, acetaldehyde and acetone in the urine of rats in response to 2,3,7,8-tetrachlorodibenzo-p-dioxin, paraquat, endrin and carbon tetrachloride.
abstract: Formaldehyde (FA), acetaldehyde (ACT), malondialdehyde (MDA) and acetone (ACON) were simultaneously identified in urine, and their excretion quantitated in response to chemically induced oxidative stress. Urine samples of female Sprague-Dawley rats were collected over dry ice and derivatized with 2,4-dinitrophenylhydrazine. The hydrazones of the four lipid metabolic products were quantitated by high-performance liquid chromatography on a Waters 10-microns mu-Bondapak C18 column. The identities of FA, ACT, MDA and ACON in urine were confirmed by gas chromatography-mass spectrometry. An oxidative stress was induced by orally administering 100 micrograms/kg 2,3,7,8-tetrachlorodibenzo-p-dioxin, 75 mg/kg paraquat, 6 mg/kg endrin or 2.5 ml/kg carbon tetrachloride to rats. Urinary excretion of FA, ACT, MDA and ACON increased relative to control animals 24 h after treatment with all xenobiotics. The system has wide-spread applicability to the investigation of altered lipid metabolism in disease states and exposure to environmental pollutants.
authors.affiliation: School of Pharmacy and Allied Health Professions, Creighton University Health Sciences Center, Omaha, NE 68178.
authors.forename: M A
authors.initials: MA
authors.lastname: Shara
authors.forename: P H
authors.initials: PH
authors.lastname: Dickson
authors.forename: D
authors.initials: D
authors.lastname: Bagchi
authors.forename: S J
authors.initials: SJ
authors.lastname: Stohs
mesh_terms: D000079:Acetaldehyde / Q000652:urine; D000096:Acetone / Q000652:urine; D000447:Aldehydes / Q000652:urine*; D000818:Animals; D002251:Carbon Tetrachloride / Q000633:toxicity; D002851:Chromatography, High Pressure Liquid; D004732:Endrin / Q000633:toxicity; D005260:Female; D005557:Formaldehyde / Q000652:urine; D008401:Gas Chromatography-Mass Spectrometry; D006835:Hydrazones / Q000737:chemistry; D008315:Malondialdehyde / Q000652:urine; D010269:Paraquat / Q000633:toxicity; D000072317:Polychlorinated Dibenzodioxins / Q000633:toxicity; D051381:Rats; D017207:Rats, Sprague-Dawley; D015262:Xenobiotics / Q000633:toxicity*
chemical_list: D000447:Aldehydes; D006835:Hydrazones; D000072317:Polychlorinated Dibenzodioxins; D015262:Xenobiotics; D000096:Acetone; D005557:Formaldehyde; D008315:Malondialdehyde; D002251:Carbon Tetrachloride; D000079:Acetaldehyde; D004732:Endrin; D010269:Paraquat