The neonatal rabbit brain glucose transporter.
The Glut 1 (Hep G2/rat brain) isoform of glucose transporter is expressed in significant amounts in adult mammalian brain. The purpose of our present study was to determine the brain cellular localization of Glut 1 during the late newborn stage of development, when brain cellular proliferation and differentiation is highly active. Employing immunohistochemistry and in-situ hybridization in 10-day-old neonatal rabbit brain sections, we undertook cellular localization of Glut 1 expression. Glut 1 protein and mRNA were mainly noted in considerable amounts within the 10-day-old brain microvasculature. Lower concentrations of Glut 1 immunoreactivity were present in certain glial cells found within the deeper cortical layers of brain. Northern blot analysis of total RNA from isolated microvasculature-enriched preparation, isolated and cultured neuronal and glial cells, whole brain and whole brain with the exclusion of microvasculature obtained from the 10-day-old, revealed the universal presence of a approximately 2.8 kb Glut 1 mRNA with the exception of the neuron-enriched cultures. We conclude that during the neonatal period, when parenchymal cellular proliferation is at a peak, Glut 1 is localized not only to the microvasculature but also to certain cells which express glial morphological characteristics. The neuronal cells either do not express Glut 1 or express minute amounts.
Devaskar
S
S
Department of Pediatrics, St. Louis University School of Medicine MO.
Chundu
K
K
Zahm
D S
DS
Holtzclaw
L
L
Holloran
K
K
D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism*; D015152:Blotting, Northern; D001921:Brain / Q000378:metabolism*; D002478:Cells, Cultured; D007124:Immunoenzyme Techniques; D009004:Monosaccharide Transport Proteins / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009693:Nucleic Acid Hybridization; D011233:Precipitin Tests; D012313:RNA / Q000302:isolation & purification; D011817:Rabbits
D009004:Monosaccharide Transport Proteins; D009419:Nerve Tissue Proteins; D012313:RNA