Monoclonal antibody epitopes of mycobacterial 65-kD heat-shock protein defined by epitope scanning.
The binding sites for MoAbs to the 65-kD heat-shock protein (hsp65) of mycobacteria have been investigated by epitope scanning. Five hundred and twenty-six 8-mer peptides representing the complete sequence of Mycobacterium tuberculosis hsp65 were synthesised in duplicate using the Epitope Scanning Kit (CRB Ltd.). The epitopes of six MoAbs raised to the hsp65 of M. tuberculosis or M. leprae were investigated. We have identified the epitope of a new MoAb (DC16); this epitope is continuous, hydrophilic in nature and 11 amino acids long. We have also confirmed the location of the epitopes of three MoAbs (IIH9, ML30 and IIC8). Thus the epitope scanning technique has proved suitable for the detection of continuous epitopes of hsp65.
Hajeer
A H
AH
Department of Rheumatology, University of Manchester Medical School, UK.
Worthington
J
J
Morgan
K
K
Bernstein
R M
RM
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000937:Antigen-Antibody Reactions; D001426:Bacterial Proteins; D001666:Binding Sites, Antibody / Q000235:genetics; D018834:Chaperonin 60; D018833:Chaperonins; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000235:genetics* / Q000276:immunology; D006360:Heat-Shock Proteins / Q000235:genetics* / Q000276:immunology*; D008969:Molecular Sequence Data; D009166:Mycobacterium leprae; D009169:Mycobacterium tuberculosis
D000911:Antibodies, Monoclonal; D001426:Bacterial Proteins; D018834:Chaperonin 60; D000939:Epitopes; D006360:Heat-Shock Proteins; C074119:heat-shock protein 65, Mycobacterium; D018833:Chaperonins