title: Ferric-ion-photosensitized damage to DNA by hydroxyl and non-hydroxyl radical mechanisms.
abstract: Iron(III) and UVA (320-400 nm) light strongly diminished the transforming activity of Haemophilus influenzae DNA in the presence of oxygen. Iron(III) alone in the absence of light had no measurable effect on the transforming activity. The chelating agent ethylenediaminetetraacetic acid (EDTA) conferred virtually complete protection, but hydroxyl radical scavengers (mannitol, methanol, ethanol, isopropanol and dimethyl sulfoxide) inhibited only a small fraction of the inactivation. Treatment of plasmid DNA (pBR322) with iron(III) results in the conversion of the covalently closed circular form of the plasmid to open circles and ultimately to the linear form. Concomitant with the alteration in the conformation of the plasmid, the ability to transform Escherichia coli was reduced. In model systems, iron(III) photoreacted with the DNA backbone causing nicking and double-strand breakage. The results are consistent with a mechanism involving a preliminary complexation of iron(III) by DNA followed by the generation of reactive free radicals other than .OH. We suggest that bound iron, or other UV-absorbing transition metal complexes, may be chromophores capable of causing DNA damage in the long-wave near-UV region.
authors.affiliation: Institute for Environmental Studies, University of Illinois, Urbana-Champaign 61801.
authors.forename: R A
authors.initials: RA
authors.lastname: Larson
authors.forename: R E
authors.initials: RE
authors.lastname: Lloyd
authors.forename: K A
authors.initials: KA
authors.lastname: Marley
authors.forename: R W
authors.initials: RW
authors.lastname: Tuveson
mesh_terms: D004249:DNA Damage; D004269:DNA, Bacterial / Q000187:drug effects* / Q000528:radiation effects; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics* / Q000528:radiation effects; D005609:Free Radicals; D006193:Haemophilus influenzae / Q000187:drug effects / Q000235:genetics* / Q000528:radiation effects; D007501:Iron / Q000494:pharmacology*; D010957:Plasmids / Q000187:drug effects / Q000528:radiation effects; D011838:Radiation-Sensitizing Agents / Q000494:pharmacology*; D014466:Ultraviolet Rays
chemical_list: D004269:DNA, Bacterial; D005609:Free Radicals; D011838:Radiation-Sensitizing Agents; D007501:Iron