title: Human serum stimulates the production of G-CSF, IL-1, IL-6 and IL-8 by human peripheral blood leucocytes.
abstract: Human serum induces human peripheral blood leucocytes (PBL) to release an activity stimulating neutrophil colony formation (G-CSA) from human bone marrow cells. By titrating individual growth factors and using specific neutralizing antibodies we showed that: human serum contains very low levels of G-CSF which are by themselves insufficient to stimulate myeloid colony formation in primary human bone marrow cultures and cannot account for the serum releaser activity; that although no detectable levels of IL-1, IL-2, IL-3, IL-4, IL-6 or IL-8 are found in the serum, anti IL-1 antibodies partially block the release of G-CSA when added early during PBL incubation; that PBL incubated in the absence of serum for 2 d produce small amounts of IL-1, IL-6, IL-8 and G-CSF and this is increased 6-16 fold in the presence of human serum; and that the neutrophil colony-stimulating activity released by PBL incubated with human serum is G-CSF.
authors.affiliation: Sandoz Pharma Ltd, Basel, Switzerland.
authors.forename: V F
authors.initials: VF
authors.lastname: Quesniaux
authors.forename: S
authors.initials: S
authors.lastname: Wehrli
authors.forename: I
authors.initials: I
authors.lastname: Ziegler
authors.forename: B
authors.initials: B
authors.lastname: Legendre
authors.forename: W
authors.initials: W
authors.lastname: Wishart
authors.forename: B
authors.initials: B
authors.lastname: Fagg
authors.forename: M H
authors.initials: MH
authors.lastname: Schreier
authors.forename: C
authors.initials: C
authors.lastname: Nissen
mesh_terms: D001681:Biological Assay; D001769:Blood; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D016179:Granulocyte Colony-Stimulating Factor / Q000097:blood*; D006801:Humans; D007375:Interleukin-1 / Q000096:biosynthesis; D015850:Interleukin-6 / Q000096:biosynthesis; D016209:Interleukin-8 / Q000096:biosynthesis; D007378:Interleukins / Q000096:biosynthesis*; D007962:Leukocytes / Q000276:immunology*
chemical_list: D007375:Interleukin-1; D015850:Interleukin-6; D016209:Interleukin-8; D007378:Interleukins; D016179:Granulocyte Colony-Stimulating Factor