[Analysis of newborn larva of Trichinella spiralis by immunoblot].
Newborn larva (NBL) antigens of Trichinella spiralis were analysed by Immunoblot, and were compared with the adult and muscle larva antigens. The SDS-PAGE patterns of NBL somatic constituents consisted of about 40 polypeptide bands, which were obviously different from those of adult and muscle larva. Immunoblot analysis indicated that immunization with NBL could induce a stage specific immune response. The molecular weight of specific NBL antigens were 129, 120, 89, 87, 79, 74, 72, 64, 58, 43, 40, 38, 34, 32, and 20kDa. But during the natural course of the infection, we could not detect the antibodies of anti-NBL in the host.
Yue
L L
LL
Department of Parasitology, Henan Medical University, Zhengzhou.
Cui
Z R
ZR
D000818:Animals; D000947:Antigens, Helminth / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D015151:Immunoblotting; D007814:Larva / Q000276:immunology; D014234:Trichinella / Q000276:immunology*
D000947:Antigens, Helminth