Binding of human alpha-interferon in the brain tissue membranes of rat.
The receptor binding characteristics of human lymphoblastoid alpha-interferon (IFN) to human Daudi cell line was compared with its binding to membrane homogenates derived from various regions of rat brain. Rat brain membranes bound specifically a detectable amount of I-125 alpha interferon revealing the receptor affinity constant (Kd) of 3.9-4.2 x 10(-10) M and the Bmax was 0.9-1.7 fmoles/mg wet weight tissue depending on the analyzed brain region (the highest Bmax was obtained in the membranes from the hypothalamus region, the lowest one from the spinal cord membranes).
Janicki
P K
PK
Department of Physiology, University of Cape Town Medical School, South Africa.
D000818:Animals; D001665:Binding Sites; D001921:Brain / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D006801:Humans; D016898:Interferon-alpha / Q000378:metabolism*; D051381:Rats; D011971:Receptors, Immunologic / Q000378:metabolism*; D017471:Receptors, Interferon; D013116:Spinal Cord / Q000378:metabolism; D014407:Tumor Cells, Cultured
D016898:Interferon-alpha; D011971:Receptors, Immunologic; D017471:Receptors, Interferon