Release of sensory CGRP by hypertonic NaCl is not blocked by tetrodotoxin, omega-conotoxin, nifedipine and ruthenium red.
Hypertonic NaCl (160 mM added to the physiological salt solution) releases CGRP in a Ca(2+)-dependent manner from capsaicin-sensitive sensory nerves of the rat urinary bladder. The NaCl (160 mM)-evoked CGRP release was not affected by tetrodotoxin (0.3 microM), nifedipine (1 microM), omega-conotoxin (0.1 microM) and ruthenium red (10 microM). NaCl (160 mM)-evokes release of sensory neuropeptides without the involvement of axon reflexes, and by promoting Ca2+ influx via a dihydropyridine omega-conotoxin and ruthenium red insensitive pathway.
Del Bianco
E
E
Institute of Internal Medicine IV, University of Florence, Italy.
Tramontana
M
M
Bertrand
C
C
Geppetti
P
P
D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000187:drug effects* / Q000378:metabolism; D002121:Calcium Channel Blockers / Q000494:pharmacology*; D066298:In Vitro Techniques; D008297:Male; D009543:Nifedipine / Q000494:pharmacology; D010456:Peptides, Cyclic / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D012430:Ruthenium Red / Q000494:pharmacology; D012462:Saline Solution, Hypertonic / Q000494:pharmacology*; D013779:Tetrodotoxin / Q000494:pharmacology*; D020960:omega-Conotoxins
D002121:Calcium Channel Blockers; D010456:Peptides, Cyclic; D012462:Saline Solution, Hypertonic; D020960:omega-Conotoxins; C053423:Conus magus toxin; D012430:Ruthenium Red; D013779:Tetrodotoxin; D009543:Nifedipine; D015740:Calcitonin Gene-Related Peptide