Processing of structural polypeptides of infectious flacherie virus of the silkworm, Bombyx mori: VP1 and VP4 are derived from VP0.
Infectious flacherie virus of the silkworm, Bombyx mori, contains five major polypeptides termed VP0, VP1, VP2, VP3, and VP4 in the order of descending molecular weight. Immunoblot analysis with specific antisera against each of these structural polypeptides showed that antisera against VP1 and VP4 unequivocally reacted with VP0 as well as their homologous structural polypeptides. Limited proteolysis of VP0 and VP1 by Staphylococcus aureus V8 protease gave several common polypeptide fragments. Amino acid sequence analysis showed that VP0 and VP4 shared a common NH2-terminal amino acid sequence. These results indicate that VP1 and VP4 are generated from VP0 and that VP4 occupies the NH2-terminal portion of VP0.
Choi
H K
HK
Laboratory of Sericultural Science, Faculty of Agriculture, Nagoya University, Japan.
Sasaki
T
T
Tomita
T
T
Kobayashi
M
M
Kawase
S
S
D000595:Amino Acid Sequence; D000818:Animals; D012831:Bombyx / Q000382:microbiology*; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry / Q000378:metabolism*; D010849:Picornaviridae / Q000378:metabolism*; D011498:Protein Precursors / Q000378:metabolism*; D015678:Viral Structural Proteins / Q000378:metabolism*
D010455:Peptides; D011498:Protein Precursors; D015678:Viral Structural Proteins