Ultraviolet B dose-dependent inflammation in humans: a reflectance spectroscopic and laser Doppler flowmetric study using topical pharmacologic antagonists on irradiated skin.
Normal skin responds acutely to ultraviolet (UV) light exposure with complex inflammatory mechanisms. In the present study UVB irradiation ranging from subclinical erythema doses to twice the minimal erythema dose (24 mJ/cm2 to 96 mJ/cm2) was delivered to the skin of 8 volunteers. Pre-irradiated sites were immediately afterwards exposed to a 24-h occlusive patch containing 1 of 4 anti-inflammatory agents or vehicle control. The resultant change in erythema (vascular reaction) was measured objectively using laser Doppler flowmetry (LDF) and reflectance spectroscopy (RS). The 4 anti-inflammatory compounds reduced the UVB-induced vascular reactions in different and dose-dependent ways. Betamethasone-17-valerate and diphenhydramine were most effective at the 24 mJ/cm2 dose site and indomethacin and acetylsalicylic acid were more effective at sites > or = 48 mJ/cm2. Ranking the reduction in oxygenized hemoglobin (OH) content was as follows: betamethasone-17-valerate (OH reduction = 37.4%) > indomethacin (OH reduction = 21.5%) > acetylsalicylic acid (ASA) (OH decrease = 21.0%) > diphenhydramine (OH reduction = 18.4%). Using LDF, the total ranking of the cutaneous blood flow (BF) reduction was: indomethacin (BF reduction = 39.7%) > betamethasone-17-valerate (BF reduction = 32.7%) > acetylsalicylic acid (BF decrease = 17.5%) > diphenhydramine (BF reduction = 12.3%). Diphenhydramine significantly reduced erythema only at the lowest irradiation dose (24 mJ/cm2) and the decrease in OH was associated with an increased amount of deoxygenized hemoglobin (DOH). A similar slight venous dilatation was present at acetylsalicylic acid-exposed sites.(ABSTRACT TRUNCATED AT 250 WORDS)
Andersen
P H
PH
Department of Dermatology, University of California, School of Medicine, San Francisco 94143-0989.
Abrams
K
K
Maibach
H
H
D000287:Administration, Topical; D000328:Adult; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage*; D001241:Aspirin / Q000008:administration & dosage; D001624:Betamethasone Valerate / Q000008:administration & dosage; D004155:Diphenhydramine / Q000008:administration & dosage; D004307:Dose-Response Relationship, Radiation; D004890:Erythema / Q000209:etiology / Q000473:pathology*; D005260:Female; D006801:Humans; D007213:Indomethacin / Q000008:administration & dosage; D017078:Laser-Doppler Flowmetry; D008875:Middle Aged; D012867:Skin / Q000098:blood supply / Q000473:pathology / Q000528:radiation effects*; D013050:Spectrometry, Fluorescence; D014466:Ultraviolet Rays
D000893:Anti-Inflammatory Agents; D004155:Diphenhydramine; D001624:Betamethasone Valerate; D001241:Aspirin; D007213:Indomethacin