title: Surface-reactive biomaterials in osteoblast cultures: an ultrastructural study.
abstract: The tissue/biomaterial interface reactions of three biomaterials selected as candidates for hard tissue replacement were studied at the electron microscopical level after incubation with enzymatically isolated rat bone cells. An electron-dense layer was routinely observed between hydroxyapatite, coral, cytodex polymer and the neighbouring cells. This layer was visible before bone formation occurred, and was collagen free. The ultrastructural features revealed a needle-shaped filamentous layer continuous with coral material, whereas hydroxyapatite or cytodex/tissue interface was granular in appearance. These different structures may indicate reactive surfaces, depending on the composition of the substrate.
authors.affiliation: Laboratoire de Biologie-Odontologie, Université Paris VII, France.
authors.forename: J M
authors.initials: JM
authors.lastname: Sautier
authors.forename: J R
authors.initials: JR
authors.lastname: Nefussi
authors.forename: N
authors.initials: N
authors.lastname: Forest
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D002478:Cells, Cultured; D003911:Dextrans; D017886:Durapatite; D006882:Hydroxyapatites; D008422:Materials Testing; D008854:Microscopy, Electron; D010006:Osteoblasts / Q000166:cytology*; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D003911:Dextrans; D006882:Hydroxyapatites; C052839:Cytodex; D017886:Durapatite