title: Cosmid pJAR4, a novel Streptomyces-Escherichia coli shuttle vector for the cloning of Streptomyces operons.
abstract: A novel shuttle cosmid vector (pJAR4), based on pK505, was constructed for the cloning of Streptomyces DNA. It is a low-copy-number vector which determines hygromycin B-resistance as a selective marker and was used to clone the puromycin biosynthesis pathway from Streptomyces alboniger. Cosmids pJAR4 and pKC505 (which determines apramycin-resistance) stably co-transform both Streptomyces lividans and Streptomyces griseofuscus.
authors.affiliation: Centro de Biología Molecular (CSIC-UAM), Universidad Autónoma de Madrid, Cantoblanco, Spain.
authors.forename: J A
authors.initials: JA
authors.lastname: Tercero
authors.forename: R A
authors.initials: RA
authors.lastname: Lacalle
authors.forename: A
authors.initials: A
authors.lastname: Jiménez
mesh_terms: D003001:Cloning, Molecular; D003360:Cosmids; D004269:DNA, Bacterial / Q000235:genetics; D004352:Drug Resistance, Microbial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D005822:Genetic Vectors; D009876:Operon; D011691:Puromycin / Q000096:biosynthesis; D015183:Restriction Mapping; D013302:Streptomyces / Q000235:genetics* / Q000378:metabolism
chemical_list: D004269:DNA, Bacterial; D011691:Puromycin