title: Atrial natriuretic factor infusion following acute renal ischemia in anesthetized dogs.
abstract: Atrial natriuretic factor (ANF) has been shown to be effective in reversing renal functional impairments following renal ischemia. We studied the effects of a nonhypotensive intravenous ANF infusion (100 ng/min x kgBW, 60 min) after 90 min unilateral renal arterial occlusion in anesthetized dogs with an intact contralateral kidney. ANF plasma levels remained unchanged in controls (group 1) and increased in ANF-infused animals (group 2) from 22 +/- 3 to 552 +/- 124 pg/ml. Blood pressure increased in both groups during renal ischemia, but returned to control values in group 2 when ANF infusion was started. Plasma vasopressin did not change in group 1, but increased in group 2 (0.77 +/- 0.29 vs. 1.10 +/- 0.49 pg/ml) after terminating ANF infusion. The postischemic fall in creatinine clearance (CCr), filtration fraction (FF) and renal blood flow (RBF) was prevented by infusion of ANF (CCr: group 1, 0.16 +/- 0.05 vs. group 2, 1.01 +/- 0.25 ml/min x kgBW; FF: group 1, 4.0 +/- 1.6 vs group 2, 14.1 +/- 4.1%; RBF: group 1, 6.0 +/- 1.2 vs. group 2, 9.2 +/- 1.6 ml/min x kgBW); however, the effects were limited to the time of infusion and the postischemic increase in urinary excretion of the proximal tubular enzyme N-acetyl-beta-D-glucosaminidase (NAG; group 1, 317.7 +/- 163.6 vs. group 2, 672.4 +/- 245.7 microU/min x kgBW) was not improved by ANF. Our data suggest that infusion of ANF transiently reverses postischemic renal impairment. However, the failure to demonstrate a sustained postischemic improvement of renal functional parameters and to ameliorate massive NAG excretion casts doubt on the benefit of ANF infusion in preventing cellular damage.
authors.affiliation: Physiologisches Institut der Universität München, FRG.
authors.forename: H G
authors.initials: HG
authors.lastname: Klein
authors.forename: D
authors.initials: D
authors.lastname: Dimitrov
authors.forename: I
authors.initials: I
authors.lastname: Atanasova
authors.forename: R M
authors.initials: RM
authors.lastname: Hohenfellner
authors.forename: U
authors.initials: U
authors.lastname: Schmausser
authors.forename: N
authors.initials: N
authors.lastname: Natcheff
authors.forename: K
authors.initials: K
authors.lastname: Thurau
mesh_terms: D000118:Acetylglucosaminidase / Q000652:urine; D058186:Acute Kidney Injury / Q000188:drug therapy*; D000818:Animals; D009320:Atrial Natriuretic Factor / Q000008:administration & dosage* / Q000097:blood; D004285:Dogs; D007511:Ischemia / Q000188:drug therapy*; D014667:Vasopressins / Q000097:blood
chemical_list: D014667:Vasopressins; D009320:Atrial Natriuretic Factor; D000118:Acetylglucosaminidase