A recA-ada hybrid gene inducible by DNA damage.
A damage-inducible expression vector was constructed in which the original recA structural gene was replaced by the protein-coding region of the ada gene. The O6-alkylguanine-DNA alkyltransferase encoded by the ada gene can be measured by a rapid and highly sensitive assay. The introduction of this construct into an appropriate host cell provides an effective bacterial assay for genotoxins.
Kleibl
K
K
Department of Molecular Genetics, Slovak Academy of Sciences, Bratislava, Czechoslovakia.
Margison
G P
GP
Skorvaga
M
M
Brozmanová
J
J
Masek
F
F
D003001:Cloning, Molecular; D004249:DNA Damage; D004926:Escherichia coli / Q000235:genetics; D005822:Genetic Vectors; D008780:Methyltransferases / Q000235:genetics*; D019853:O(6)-Methylguanine-DNA Methyltransferase; D010957:Plasmids; D011938:Rec A Recombinases / Q000235:genetics*; D012045:Regulatory Sequences, Nucleic Acid; D013014:SOS Response, Genetics / Q000235:genetics*
D008780:Methyltransferases; D019853:O(6)-Methylguanine-DNA Methyltransferase; D011938:Rec A Recombinases