title: Capillary gas-liquid chromatographic separation of bile alcohols.
abstract: Gas-liquid chromatographic separation of C23, C24, C25, C26, and C27 bile alcohols with either 3 alpha, 7 alpha-dihydroxylated or 3 alpha, 7 alpha, 12 alpha-trihydroxylated ring system on two capillary columns, CP-Sil-19 CB and CP-Sil-5 CB, is described. Bile alcohols with two ring hydroxyl groups at 3 alpha- and 7 alpha-positions consistently showed larger retention times on CP-Sil-19 CB columns and smaller retention times on CP-Sil-5 CB columns than the corresponding bile alcohols with three ring hydroxyl groups at 3 alpha-, 7 alpha-, and 12 alpha-positions. Resolutions of all bile alcohols were better on CP-Sil-19 CB columns; however, we hope that the gas-liquid chromatographic characteristics on the two columns will be useful for better identification of bile alcohols in biological fluids.
authors.affiliation: Department of Medicine, University of Medicine and Dentistry of New Jersey, New Jersey Medical School, Newark 07103.
authors.forename: A K
authors.initials: AK
authors.lastname: Batta
authors.forename: S K
authors.initials: SK
authors.lastname: Aggarwal
authors.forename: R
authors.initials: R
authors.lastname: Mirchandani
authors.forename: S
authors.initials: S
authors.lastname: Shefer
authors.forename: G
authors.initials: G
authors.lastname: Salen
mesh_terms: D002777:Cholestanols / Q000737:chemistry / Q000302:isolation & purification* / Q000652:urine; D002849:Chromatography, Gas / Q000379:methods*; D006801:Humans; D015394:Molecular Structure; D014973:Xanthomatosis / Q000652:urine
chemical_list: D002777:Cholestanols