Translation of the prophage lambda cl transcript.
Mutations in rpsB that reduce the levels of the ribosomal protein S2 enhance the translation of cl in lambda lysogens. Two features of the cl transcript are required for enhanced translation: the absence of a leader and the presence of a downstream box, a sequence within the cl coding region that is complementary to the 16S rRNA. 30S ribosomal subunits deficient in S2 form ternary complexes with the cl transcript more efficiently than wild-type subunits. The absence of S2 may change the structure of the 16S rRNA, improving contacts with the cl downstream box.
Shean
C S
CS
Institute of Cancer Research, Columbia University College of Physicians and Surgeons, New York, New York 10032.
Gottesman
M E
ME
D010582:Bacteriophage lambda / Q000235:genetics*; D001483:Base Sequence; D015153:Blotting, Western; D003062:Codon; D004277:DNA, Single-Stranded; D004268:DNA-Binding Proteins; D004926:Escherichia coli / Q000235:genetics; D005814:Genes, Viral; D008969:Molecular Sequence Data; D009154:Mutation; D010957:Plasmids; D014176:Protein Biosynthesis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics; D012045:Regulatory Sequences, Nucleic Acid; D012097:Repressor Proteins / Q000235:genetics*; D012269:Ribosomal Proteins / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic; D014764:Viral Proteins; D054334:Viral Regulatory and Accessory Proteins; D001616:beta-Galactosidase / Q000235:genetics
D003062:Codon; D004277:DNA, Single-Stranded; D004268:DNA-Binding Proteins; D012336:RNA, Ribosomal, 16S; D012367:RNA, Viral; D012097:Repressor Proteins; D012269:Ribosomal Proteins; D014764:Viral Proteins; D054334:Viral Regulatory and Accessory Proteins; C016649:phage repressor proteins; C030004:ribosomal protein S2; D001616:beta-Galactosidase