title: A new restriction fragment length polymorphism of the human alpha-fetoprotein gene in Japanese individuals: detection of loss of heterozygosity in human hepatocellular carcinoma.
abstract: By using the cDNA probe for the human alpha-fetoprotein (AFP) gene, one restriction fragment length polymorphism (RFLP) was discovered in Japanese patients. A variety of combinations of restriction enzymes and probes were evaluated in DNA samples from 25 Japanese individuals who were genetically unrelated. As a result, AvaII identified one invariant band of 8 kb and polymorphic bands at either 2.8 or 2.0 kb with the 5'-region probe from a cDNA clone, pHAF7. With the AvaII/pHAF7 combination, we examined the loss of heterozygosity in 14 patients with hepatocellular carcinoma (HCC). Of 6 informative specimens, 2 showed loss of heterozygosity. These data suggest that the AFP cDNA probe could be useful for analyzing loss of heterozygosity on chromosome 4q in human HCC.
authors.affiliation: Department of Internal Medicine, Sapporo Medical College, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Toyota
authors.forename: Y
authors.initials: Y
authors.lastname: Hinoda
authors.forename: T
authors.initials: T
authors.lastname: Nakano
authors.forename: K
authors.initials: K
authors.lastname: Imai
authors.forename: A
authors.initials: A
authors.lastname: Yachi
mesh_terms: D000368:Aged; D006528:Carcinoma, Hepatocellular / Q000235:genetics*; D002872:Chromosome Deletion; D006580:Genetic Carrier Screening; D006579:Heterozygote; D006801:Humans; D007564:Japan / Q000208:ethnology; D008113:Liver Neoplasms / Q000235:genetics*; D008875:Middle Aged; D012150:Polymorphism, Restriction Fragment Length; D000509:alpha-Fetoproteins / Q000235:genetics*
chemical_list: D000509:alpha-Fetoproteins