Nuclear translocation of monoclonal antibody directed against cell-surface carbohydrate Y determinant.
Monoclonal antibody (MAb) Br 15-6A directed against the carbohydrate Y determinant expressed on tumor cells was found to be internalized and translocated to the nucleus of SK Br 5 breast carcinoma and SW 1116 and SW 707 colorectal carcinoma cells. Intracellular localization of MAb Br 15-6A was determined by cell fraction and by indirect immunofluorescence staining. Internalization of MAb Br 15-6A seems to be mediated by a specific cell surface protein of M(r) 108,000 in colorectal carcinoma cells and M(r) 92,000-96,000 in breast carcinoma cells. The MAb Br 15-6A precipitates an 88,000 M(r) chromatin protein and appears to be bound specifically to two EcoRI-digested and two HincII-chromatin fragments. Another MAb against the Y determinant (MAb Br 55.2) recognizes the same antigens as MAb Br 15-6A, but is not internalized.
Rakowicz-Szulczynska
E M
EM
Wistar Institute of Anatomy and Biology, Philadelphia, Pennsylvania.
Steplewski
Z
Z
Koprowski
H
H
D000911:Antibodies, Monoclonal / Q000378:metabolism; D001943:Breast Neoplasms / Q000378:metabolism*; D050260:Carbohydrate Metabolism; D002462:Cell Membrane; D002467:Cell Nucleus / Q000378:metabolism; D002843:Chromatin / Q000378:metabolism; D015179:Colorectal Neoplasms / Q000378:metabolism*; D000939:Epitopes; D006801:Humans; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D002843:Chromatin; D000939:Epitopes