Periodic acid thiosemicarbazide gelatin methenamine silver (PATSC-GMS) staining for transmission electron microscopy.
A new electron microscopic method is described in which superior staining of basement membranes, collagen fibers and glycogen particles in various tissues was achieved. This method uses a gelatin methenamine silver solution applied to the ultrathin sections on nickel grids after oxidation in a periodic acid. The staining results were excellent and tissue components were evenly stained with no background silver grains. This method proved to be consistent, reproducible and reliable.
Namimatsu
S
S
Division of Electron Microscopy, First Hospital, Nippon Medical School, Tokyo, Japan.
D000818:Animals; D002462:Cell Membrane / Q000737:chemistry / Q000648:ultrastructure; D003094:Collagen / Q000032:analysis / Q000648:ultrastructure; D005780:Gelatin; D006003:Glycogen / Q000032:analysis; D006801:Humans; D007668:Kidney / Q000737:chemistry / Q000648:ultrastructure; D008099:Liver / Q000737:chemistry / Q000648:ultrastructure; D008168:Lung / Q000737:chemistry / Q000648:ultrastructure; D008709:Methenamine; D008854:Microscopy, Electron / Q000379:methods*; D010504:Periodic Acid; D012663:Semicarbazides; D013194:Staining and Labeling / Q000379:methods*
D012663:Semicarbazides; D010504:Periodic Acid; C005151:thiosemicarbazide; D005780:Gelatin; D006003:Glycogen; D003094:Collagen; D008709:Methenamine