Platelet-derived growth factor BB-dimer suppresses the expression of macrophage colony-stimulating factor in human vascular smooth muscle cells.
Vascular smooth muscle cell is a major cell component involved in the process of atherosclerosis. In the present study, we investigated the effects of platelet-derived growth factor (PDGF)-BB dimer on the expression of macrophage-colony stimulating factor (M-CSF) in vascular smooth muscle cells isolated from human umbilical artery. On Northern blot analysis of total RNAs isolated from smooth muscle cells, with human cDNA for M-CSF, a marked dose-dependent reduction of mRNA level was found in PDGF-BB-treated smooth muscle cells. Cellular production of M-CSF was estimated by immunoblot analysis of cell lysate with specific polyclonal antibody against recombinant human M-CSF. A concentration of 10 ng/ml PDGF-BB significantly reduced M-CSF mass in smooth muscle cells compared with that in the absence of PDGF-BB. These results suggest that PDGF-BB plays an important role in the cellular metabolism of vascular wall by regulating the rate of M-CSF production in vascular smooth muscle cells.
Shimada
M
M
Third Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Japan.
Inaba
T
T
Shimano
H
H
Gotoda
T
T
Watanabe
Y
Y
Yamamoto
K
K
Motoyoshi
K
K
Yazaki
Y
Y
Yamada
N
N
D000906:Antibodies; D015152:Blotting, Northern; D015153:Blotting, Western; D002478:Cells, Cultured; D015870:Gene Expression; D006801:Humans; D046911:Macromolecular Substances; D016173:Macrophage Colony-Stimulating Factor / Q000096:biosynthesis / Q000235:genetics* / Q000494:pharmacology; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000502:physiology*; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology*; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000494:pharmacology; D014469:Umbilical Arteries
D000906:Antibodies; D046911:Macromolecular Substances; D010982:Platelet-Derived Growth Factor; D012333:RNA, Messenger; D011994:Recombinant Proteins; D012313:RNA; D016173:Macrophage Colony-Stimulating Factor