The three members of the selectin receptor family recognize a common carbohydrate epitope, the sialyl Lewis(x) oligosaccharide.
The selectins (lectin-EGF-complement binding-cell adhesion molecules [LEC-CAMs]) are a family of mammalian receptors implicated in the initial interactions between leukocytes and vascular endothelia, leading to lymphocyte homing, platelet binding, and neutrophil extravasation. The three known selectins, L-selectin (leukocyte adhesion molecule-1 [LECAM-1]), E-selectin (endothelial-leukocyte adhesion molecule-1 [ELAM-1]), and P-selectin (GMP-140) share structural features that include a calcium-dependent lectin domain. The sialyl Lewis(x) carbohydrate epitope has been reported as a ligand for both E- and P-selectins. Although L-selectin has been demonstrated to bind to carbohydrates, structural features of potential mammalian carbohydrate ligand(s) have not been well defined. Using an ELISA developed with a sialyl Lewis(x)-containing glycolipid and an E-selectin-IgG chimera, we have demonstrated the direct binding of the L-selectin-IgG chimera to sialyl Lewis(x). This recognition was calcium dependent, and could be blocked by Mel-14 antibody but not by other antibodies. Recognition was confirmed by the ability of cells expressing the native L-selectin to adhere to immobilized sialyl Lewis(x). These data suggest that the sialyl Lewis(x) oligosaccharide may form the basis of a recognition domain common to all three selectins.
Foxall
C
C
Glycomed, Inc., Alameda, California 94501.
Watson
S R
SR
Dowbenko
D
D
Fennie
C
C
Lasky
L A
LA
Kiso
M
M
Hasegawa
A
A
Asa
D
D
Brandley
B K
BK
D000818:Animals; D002240:Carbohydrate Sequence; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D002478:Cells, Cultured; D019040:E-Selectin; D000939:Epitopes; D005732:Gangliosides / Q000737:chemistry / Q000378:metabolism*; D066298:In Vitro Techniques; D019041:L-Selectin; D016256:Lewis X Antigen / Q000378:metabolism*; D008024:Ligands; D051379:Mice; D008969:Molecular Sequence Data; D019007:P-Selectin; D010980:Platelet Membrane Glycoproteins / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000378:metabolism
D015815:Cell Adhesion Molecules; D019040:E-Selectin; D000939:Epitopes; D005732:Gangliosides; D016256:Lewis X Antigen; D008024:Ligands; D019007:P-Selectin; D010980:Platelet Membrane Glycoproteins; D011993:Recombinant Fusion Proteins; D019041:L-Selectin