title: IGFBP-3 gene expression and estrogen receptor status in human breast carcinoma.
abstract: Insulin-like growth factors (IGF) I and II are potent mitogens for breast carcinoma proliferation. IGF-mediated proliferative activity can be markedly enhanced by the presence of specific IGF-binding proteins (IGFBPs). IGFBP-3 has been shown to enhance IGF-mediated growth in a number of systems. Studies have demonstrated IGFBP-3 secretion only in estrogen receptor (ER)-negative breast carcinoma cell lines while IGFBP-3 could not be detected in media conditioned by ER-positive cell lines. We investigated whether a relationship exists between ER status and IGFBP-3 mRNA expression in human breast carcinoma biopsy specimens. We have detected IGFBP-3 mRNA in breast carcinoma tissue obtained from patients utilizing in situ hybridization. Quantitation of IGFBP-3 mRNA levels was performed utilizing image cytometry. There was a significantly higher expression of IGFBP-3 mRNA in ER-negative breast carcinoma specimens when compared to the ER-positive specimens. Whether this higher expression of IGFBP-3 mRNA and presumed secretion of IGFBP-3 by ER-negative tumors play a role in the rapid proliferation and poor prognosis of these tumors remains to be determined.
authors.affiliation: Department of Medicine, University of Maryland, Baltimore 21201.
authors.forename: Z M
authors.initials: ZM
authors.lastname: Shao
authors.forename: M S
authors.initials: MS
authors.lastname: Sheikh
authors.forename: J V
authors.initials: JV
authors.lastname: Ordonez
authors.forename: P
authors.initials: P
authors.lastname: Feng
authors.forename: T
authors.initials: T
authors.lastname: Kute
authors.forename: J C
authors.initials: JC
authors.lastname: Chen
authors.forename: S
authors.initials: S
authors.lastname: Aisner
authors.forename: L
authors.initials: L
authors.lastname: Schnaper
authors.forename: D
authors.initials: D
authors.lastname: LeRoith
authors.forename: C T
authors.initials: CT
authors.lastname: Roberts
mesh_terms: D001943:Breast Neoplasms / Q000235:genetics*; D002277:Carcinoma / Q000235:genetics*; D002352:Carrier Proteins / Q000378:metabolism*; D015870:Gene Expression; D006801:Humans; D018969:Insulin-Like Growth Factor Binding Proteins; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000235:genetics; D011960:Receptors, Estrogen / Q000378:metabolism*; D011980:Receptors, Progesterone / Q000378:metabolism; D016196:S Phase; D013002:Somatomedins / Q000378:metabolism
chemical_list: D002352:Carrier Proteins; D018969:Insulin-Like Growth Factor Binding Proteins; D012333:RNA, Messenger; D011960:Receptors, Estrogen; D011980:Receptors, Progesterone; D013002:Somatomedins