title: Variation in the antigenic characteristics of venom from the Mojave rattlesnake (Crotalus scutulatus scutulatus).
abstract: Venoms from 31 specimens of the Mojave rattlesnake (Crotalus scutulatus scutulatus) were examined to further characterize reported differences among venoms of this species. Twenty-two venoms were recognized by a monoclonal antibody to Mojave toxin, CSS12. Nine venoms were recognized by CA-P-8, a monoclonal antibody produced against the hemorrhagic venom of C. atrox. Seven of these produced strong hemorrhage in mice and were also recognized by polyclonal antibodies (anti-F5) produced against a fraction of Mojave rattlesnake venom that inactivates serum complement. Fractionated venom revealed that CA-P-8 and anti-F5 recognized different proteins. Two of the venoms recognized by CA-P-8 were not recognized by anti-F5 and produced minimal hemorrhage in mice. This suggests that more than one factor may be necessary to induce strong hemorrhage.
authors.affiliation: Department of Biology, Texas A&I University, Kingsville 78363.
authors.forename: S Y
authors.initials: SY
authors.lastname: Huang
authors.forename: J C
authors.initials: JC
authors.lastname: Perez
authors.forename: E D
authors.initials: ED
authors.lastname: Rael
authors.forename: C
authors.initials: C
authors.lastname: Lieb
authors.forename: M
authors.initials: M
authors.lastname: Martinez
authors.forename: S A
authors.initials: SA
authors.lastname: Smith
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000940:Antigenic Variation; D002851:Chromatography, High Pressure Liquid; D003435:Crotalid Venoms / Q000276:immunology* / Q000633:toxicity; D000939:Epitopes / Q000032:analysis*; D006470:Hemorrhage / Q000139:chemically induced; D015151:Immunoblotting; D007525:Isoelectric Focusing; D051379:Mice; D008807:Mice, Inbred BALB C; D012911:Snakes
chemical_list: D000911:Antibodies, Monoclonal; D003435:Crotalid Venoms; D000939:Epitopes