Activation of DNA cleavage by dynemicin A in a B-Z conformational junction.
We report here that the DNA strand scission by dynemicin A is not only sequence-specific but also conformation-specific. The salt-induced B----Z conformational transition dramatically enhanced the cleavage by dynemicin A in a B-Z junction region. By contrast, the bleomycin-Fe(II) complex, the elsamicin A-Fe(II) complex, and esperamicin A1 did not induce any preferential DNA cutting in such a DNA structure. The characteristic hyperreactivity of dynemicin A is observed in (dC-dG)8- and (dC-dG)12-inserted DNAs, but not in (dC-dG)5-inserted DNA. These results suggest value in the use of dynemicin A as proof of the existence of a B-Z junction in vivo and also may aid in understanding the structure of B-Z junctions.
Ichikawa
A
A
Institute for Chemical Research, Kyoto University, Japan.
Kuboya
T
T
Aoyama
T
T
Sugiura
Y
Y
D000617:Aminoglycosides; D000880:Anthraquinones; D000900:Anti-Bacterial Agents; D000903:Antibiotics, Antineoplastic; D001483:Base Sequence; D001761:Bleomycin / Q000031:analogs & derivatives; D004247:DNA / Q000737:chemistry*; D004249:DNA Damage; D004591:Electrophoresis, Polyacrylamide Gel; D053281:Enediynes; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D010957:Plasmids; D015183:Restriction Mapping
D000617:Aminoglycosides; D000880:Anthraquinones; D000900:Anti-Bacterial Agents; D000903:Antibiotics, Antineoplastic; D053281:Enediynes; D009838:Oligodeoxyribonucleotides; C023503:iron bleomycin; D001761:Bleomycin; C052201:oligo(dG-dC); D004247:DNA; C061229:dynemicin A; C047543:esperamicin A1; C049904:elsamicin A