Heart protection by cardioplegic solutions containing oxyhemoglobin pretreated by carbontetrachloride and freeze-drying with sucrose.
A technologically improved variant of native stroma-free oxyhemoglobin (SFH) pretreated by carbontetrachloride and freeze-drying with 240 mM sucrose were reconstituted in a properly diluted ionic solution to reach the final concentration of 66 g oxyhemoglobin/L, osmolality 280-320 m0sm and pH 7.4. Cardioplegia of isolated rat heart was induced and maintained by this solution without recirculation for 3 h at 20 degrees C prior to heterotopic allo-transplantation of the graft. Evaluation of the survival and performance of each graft after 24 h and extent of tissue necroses indicated that the given standardly produced SFH variant ensured reproducible heart preservation from ischemic and reperfusion injury similarly as did the renown crystalloid cardioplegic solution CUSTODIOL.
Pristoupil
T I
TI
Institute of Hematology and Blood Transfusion, Prague, CSFR.
Stĕrbíková
J
J
Vrána
M
M
Havlícková
J
J
Matĕjcková
M
M
Schejbalová
S
S
Eserová
L
L
D000818:Animals; D001802:Blood Substitutes / Q000302:isolation & purification / Q000627:therapeutic use*; D002251:Carbon Tetrachloride; D002314:Cardioplegic Solutions / Q000627:therapeutic use*; D005612:Freeze Drying; D006085:Graft Survival / Q000187:drug effects; D006321:Heart / Q000187:drug effects* / Q000502:physiology; D016027:Heart Transplantation; D006454:Hemoglobins / Q000302:isolation & purification / Q000627:therapeutic use*; D006801:Humans; D066298:In Vitro Techniques; D015428:Myocardial Reperfusion Injury / Q000517:prevention & control; D009926:Organ Preservation / Q000379:methods; D010108:Oxyhemoglobins / Q000302:isolation & purification / Q000627:therapeutic use*; D051381:Rats; D013395:Sucrose
D001802:Blood Substitutes; D002314:Cardioplegic Solutions; D006454:Hemoglobins; D010108:Oxyhemoglobins; C016484:hemoglobin, stroma free; D013395:Sucrose; D002251:Carbon Tetrachloride