Characterization of the endogenous reverse transcriptase reaction products of SIVagmTYO-7, a simian immunodeficiency virus isolated from an African green monkey.
The products of the endogenous reverse transcriptase reaction of SIVagmTYO-7 were characterized after the reaction conditions had been optimized. The major reaction product in the presence of actinomycin D and oligo(dT) was a DNA with a size of 300 bases. Without actinomycin D two additional reaction products with 600 or 700 bases appeared. The 300 base product was identified as the (-)strong-stop DNA, whereas the 700 base product is the (+)strong-stop DNA. The 600 base product appeared only after oligo(dT) priming. The (-)strong-stop DNA hybridized specifically with a 9 kb RNA found in virus particles and three RNA species of 1.8, 4.8 and 9 kb isolated from SIVagmTYO-7 infected cells.
Lüke
W
W
Deutsches Primatenzentrum, Abteilung Virologie und Immunologie, Göttingen, Germany.
Dirks
B
B
Moosmayer
D
D
Jentsch
K D
KD
Hunsmann
G
G
D000818:Animals; D015152:Blotting, Northern; D002460:Cell Line; D002522:Chlorocebus aethiops; D015345:Oligonucleotide Probes; D012367:RNA, Viral / Q000737:chemistry / Q000302:isolation & purification; D012194:RNA-Directed DNA Polymerase / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D015302:Simian Immunodeficiency Virus / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification
D015345:Oligonucleotide Probes; D012367:RNA, Viral; D012194:RNA-Directed DNA Polymerase