Expression of natriuretic peptide genes in cardiac tissues of hypertensive rats.
A quantitative assay using a reverse transcriptase-linked polymerase chain reaction has been developed for measuring the levels of rANP and iso-rANP mRNA. A linear correlation between total RNA template and amplified cDNA was obtained for the amplification of cDNA from both iso-rANP and rANP mRNAs even when both cDNAs were amplified in the same assay. Application of the assay showed that in contrast to rANP levels of iso-rANP transcript in hypertensive rats remained approximately the same in atrium but were increased 10-fold in ventricle compared to normal rats. Given the relative size of the ventricle the increase of iso-rANP in this tissue in SHR may be a major response to the hypertensive state.
Roy
R N
RN
Department of Biochemistry, Queen's University, Kingston, Ontario, Canada.
Pang
S C
SC
Flynn
T G
TG
D000818:Animals; D009320:Atrial Natriuretic Factor / Q000096:biosynthesis* / Q000235:genetics; D001483:Base Sequence; D004247:DNA / Q000235:genetics; D015870:Gene Expression; D006325:Heart Atria / Q000378:metabolism; D006352:Heart Ventricles / Q000378:metabolism; D008969:Molecular Sequence Data; D009206:Myocardium / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D011918:Rats, Inbred SHR / Q000378:metabolism*; D011921:Rats, Inbred WKY / Q000378:metabolism
D012333:RNA, Messenger; D009320:Atrial Natriuretic Factor; D004247:DNA