In vitro activation of dinitrogenase reductase from the cyanobacterium Anabaena variabilis (ATCC 29413).
Nitrogenase of the heterocystous cyanobacterium Anabaena variabilis was inactivated in vivo (S. Reich, H. Almon, and P. Böger, FEMS Microbiol. Lett. 34:53-56, 1986). Partially purified and modified (inactivated) dinitrogenase reductase (Fe-protein) of such cells was reactivated by isolated membrane fractions of A. variabilis or of Rhodospirillum rubrum, and acetylene reduction was measured. Reactivation requires ATP, Mg2+, and Mn2+. The activating principle is localized in the heterocyst and was found effective only when prepared from cells exhibiting active nitrogenase. It also restores the activity of modified Fe-protein from R. rubrum.
Böhm
I
I
Lehrstuhl für Physiologie und Biochemie Pflanzen, Universität Konstanz, Germany.
Halbherr
A
A
Smaglinski
S
S
Ernst
A
A
Böger
P
P
D000114:Acetylene / Q000032:analysis; D000255:Adenosine Triphosphate / Q000378:metabolism; D017033:Anabaena / Q000201:enzymology*; D017037:Dinitrogenase Reductase / Q000302:isolation & purification / Q000378:metabolism*; D004789:Enzyme Activation; D004793:Enzyme Reactivators / Q000378:metabolism; D066298:In Vitro Techniques; D008274:Magnesium / Q000378:metabolism; D008345:Manganese / Q000378:metabolism; D009591:Nitrogenase / Q000378:metabolism*; D012247:Rhodospirillum rubrum / Q000201:enzymology
D017037:Dinitrogenase Reductase; D004793:Enzyme Reactivators; D008345:Manganese; D000255:Adenosine Triphosphate; D009591:Nitrogenase; D008274:Magnesium; D000114:Acetylene