Characterization of carbohydrates in the alpha 2-macroglobulin receptor.
Carbohydrates were characterized in the human placental alpha 2-macroglobulin receptor and its associated protein. Carbohydrates, largely N-linked, contributed to about 18% of the size of the receptor alpha-chain and to about 25% of the beta-chain. The 40 kDa receptor-associated protein also contained carbohydrate. The alpha- and beta-chains contained a wide variety of carbohydrates as judged by binding of lectins. Monosaccharide-competing inhibition of alpha 2M-methylamine binding by WGA suggested a functional significance of sugars in binding of ligands to the alpha-chain.
Jensen
P H
PH
Institute of Medical Biochemistry, University of Aarhus, Denmark.
Gliemann
J
J
Orntoft
T
T
D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000032:analysis*; D004586:Electrophoresis; D005260:Female; D006801:Humans; D037102:Lectins / Q000378:metabolism; D026503:Low Density Lipoprotein Receptor-Related Protein-1; D008969:Molecular Sequence Data; D010920:Placenta / Q000737:chemistry*; D011247:Pregnancy; D011971:Receptors, Immunologic / Q000737:chemistry* / Q000378:metabolism; D011973:Receptors, LDL / Q000378:metabolism; D000511:alpha-Macroglobulins / Q000378:metabolism*
D002241:Carbohydrates; D037102:Lectins; D026503:Low Density Lipoprotein Receptor-Related Protein-1; D011971:Receptors, Immunologic; D011973:Receptors, LDL; D000511:alpha-Macroglobulins