title: Nucleotide sequence of a cDNA clone encoding a major allergenic protein in rice seeds. Homology of the deduced amino acid sequence with members of alpha-amylase/trypsin inhibitor family.
abstract: A cDNA clone of rice major allergenic protein (RAP) was isolated from a cDNA library of maturing rice seeds. The cDNA had an open reading frame (486 nucleotides) which coded a 162 amino acid residue polypeptide comprising a 27-residue signal peptide and a 135-residue mature protein of M(r) 14,764. The deduced amino acid sequence of RAP showed a considerable similarity to barley trypsin inhibitor [1983, J. Biol. Chem. 258, 7998-8003] and wheat alpha-amylase inhibitor [1981, Phytochemistry 20, 1781-1784].
authors.affiliation: Department of Food Science and Technology, School of Agriculture, Nagoya University, Aichi, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Izumi
authors.forename: T
authors.initials: T
authors.lastname: Adachi
authors.forename: N
authors.initials: N
authors.lastname: Fujii
authors.forename: T
authors.initials: T
authors.lastname: Matsuda
authors.forename: R
authors.initials: R
authors.lastname: Nakamura
authors.forename: K
authors.initials: K
authors.lastname: Tanaka
authors.forename: A
authors.initials: A
authors.lastname: Urisu
authors.forename: Y
authors.initials: Y
authors.lastname: Kurosawa
mesh_terms: D000485:Allergens / Q000737:chemistry*; D000595:Amino Acid Sequence; D000681:Amylases / Q000037:antagonists & inhibitors* / Q000737:chemistry; D052179:Antigens, Plant; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000737:chemistry*; D008969:Molecular Sequence Data; D012275:Oryza / Q000737:chemistry*; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics*; D012639:Seeds / Q000737:chemistry*; D012689:Sequence Homology, Nucleic Acid; D014361:Trypsin Inhibitors / Q000737:chemistry*; D000516:alpha-Amylases / Q000037:antagonists & inhibitors
chemical_list: D000485:Allergens; D052179:Antigens, Plant; D010940:Plant Proteins; D014361:Trypsin Inhibitors; C074979:major allergenic protein, Oryza sativa; D004247:DNA; D000681:Amylases; D000516:alpha-Amylases