Human T lymphotropic virus types I- and II-specific antibody-dependent cellular cytotoxicity: strain specificity and epitope mapping.
Antibody-dependent cellular cytotoxicity (ADCC) against human T lymphotropic virus (HTLV) types I and II was investigated using sera obtained from infected individuals or from rabbits immunized with HTLV-I or -II envelope peptides. Target cells included an HTLV-I-transformed cell line (C91/PL), an HTLV-II-transformed cell line (729pH6neo), and Epstein Barr virus (EBV)-transformed B lymphocytes expressing HTLV-I or -II env or gag gene products after infection with vaccinia/HTLV recombinants. ADCC activity was directed at HTLV-I and -II envelope glycoproteins but not against core (gag) components. In contrast to the human immunodeficiency virus system, significant cross-reactivity between HTLV-I- and -II-directed ADCC activity was observed. Epitope mapping studies using sera from rabbits that had been immunized with HTLV-I or -II envelope peptides suggested that the critical epitopes for ADCC activity are located primarily in hydrophilic regions of the exterior (gp46) part of the envelope glycoprotein.
Zhang
X Q
XQ
Infectious Disease Division, University of Colorado Health Sciences Center, Denver 80262.
Yang
L
L
Ho
D D
DD
Kuritzkes
D R
DR
Chen
I S
IS
Ching
W T
WT
Chen
Y M
YM
Schooley
R T
RT
D000818:Animals; D000920:Antibody-Dependent Cell Cytotoxicity; D002461:Cell Line, Transformed; D003429:Cross Reactions; D015480:Deltaretrovirus Antibodies / Q000276:immunology; D015485:Deltaretrovirus Antigens / Q000032:analysis*; D000939:Epitopes / Q000032:analysis; D005455:Fluorescent Antibody Technique; D015686:Gene Products, env / Q000276:immunology; D015683:Gene Products, gag / Q000276:immunology; D015481:HTLV-I Antibodies / Q000276:immunology; D015486:HTLV-I Antigens / Q000032:analysis; D015482:HTLV-II Antibodies / Q000276:immunology; D015487:HTLV-II Antigens / Q000032:analysis; D015368:Human T-lymphotropic virus 1 / Q000276:immunology*; D015367:Human T-lymphotropic virus 2 / Q000276:immunology*; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D011817:Rabbits; D015531:Radioimmunoprecipitation Assay; D013045:Species Specificity
D015480:Deltaretrovirus Antibodies; D015485:Deltaretrovirus Antigens; D000939:Epitopes; D015686:Gene Products, env; D015683:Gene Products, gag; D015481:HTLV-I Antibodies; D015486:HTLV-I Antigens; D015482:HTLV-II Antibodies; D015487:HTLV-II Antigens; D007106:Immune Sera