Inhibition of growth factor binding and intracellular Ca2+ signalling by dextran sulfates of different sizes and degrees of sulfation.
The ability of dextran sulfates of varying molecular sizes (5-500 kDa) and degrees of sulfate substitution (0.3-1.9) to inhibit the binding of platelet-derived growth factor (PDGF) to intact Swiss 3T3 fibroblasts and to inhibit inositol(1,4,5)trisphosphate-dependent release of Ca2+ in permeabilized Swiss 3T3 cells was examined in the present study. Significant correlations were found between increased molecular size of the dextran sulfates and inhibition of both PDGF binding (r = 0.77) and Ca2+ release (r = 0.72). The degree of sulfate substitution did not correlate with inhibition of either activity.
Powis
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Arizona Cancer Center, Arizona Health Sciences Center, Tucson 85724.
Seewald
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Hoke
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D016475:3T3 Cells / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D016264:Dextran Sulfate / Q000494:pharmacology*; D015544:Inositol 1,4,5-Trisphosphate / Q000378:metabolism; D051379:Mice; D010982:Platelet-Derived Growth Factor / Q000378:metabolism*; D017479:Receptors, Platelet-Derived Growth Factor / Q000187:drug effects*; D015398:Signal Transduction / Q000187:drug effects
D010982:Platelet-Derived Growth Factor; D015544:Inositol 1,4,5-Trisphosphate; D016264:Dextran Sulfate; D017479:Receptors, Platelet-Derived Growth Factor; D002118:Calcium