Isoenzyme analysis of Hammondia hammondi and Toxoplasma gondii sporozoites.
Isoenzyme analysis using isoelectrofocusing in polyacrylamide gels was used to distinguish Hammondia hammondi and Toxoplasma gondii sporozoites. Five enzyme systems were studied: aconitase (EC 4.2.1.3), aspartate aminotransferase (EC 2.6.1.1), glucose phosphate isomerase (EC 5.3.1.9), lactate dehydrogenase (EC 1.1.1.27), and phosphoglucomutase (EC 2.7.5.1). Three stocks of T. gondii belonging to 3 zymodemes were compared to 1 stock of H. hammondi. Hammondia hammondi differed from T. gondii at all 5 loci analyzed. This was observed for all 3 zymodemes of T. gondii. These results indicated clear genetic differences between the 2 species.
Dardé
M L
ML
Service de Parasitologie-Mycologie, CHU Dupuytren, Limoges, France.
Riahi
H
H
Bouteille
B
B
Pestre-Alexandre
M
M
D000154:Aconitate Hydratase / Q000032:analysis; D000818:Animals; D001219:Aspartate Aminotransferases / Q000032:analysis; D002371:Cat Diseases / Q000469:parasitology; D002415:Cats; D003044:Coccidia / Q000145:classification / Q000201:enzymology* / Q000235:genetics; D003048:Coccidiosis / Q000469:parasitology / Q000662:veterinary; D005956:Glucose-6-Phosphate Isomerase / Q000032:analysis; D007525:Isoelectric Focusing; D007527:Isoenzymes / Q000032:analysis*; D007770:L-Lactate Dehydrogenase / Q000032:analysis; D051379:Mice; D010733:Phosphoglucomutase / Q000032:analysis; D014122:Toxoplasma / Q000145:classification / Q000201:enzymology* / Q000235:genetics; D014124:Toxoplasmosis, Animal / Q000469:parasitology
D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase; D001219:Aspartate Aminotransferases; D000154:Aconitate Hydratase; D005956:Glucose-6-Phosphate Isomerase; D010733:Phosphoglucomutase