Differential expression of insulin-like growth factor binding proteins in human non-small cell lung cancer cell lines.
The possible expression and secretion of insulin-like growth factor binding proteins (IGFBPs) by non-small cell lung cancer (NSCLC) cell lines was investigated and compared with possible IGFBP expression by primary NSCLC tumours. Cells growing under serum-free conditions released binding proteins with apparent molecular masses of 26-43 kD when analysed by a ligand blotting method under non-reducing conditions. Additionally, northern blot analysis of total RNA from NSCLC cell lines and tumours was performed using cDNAs coding for each of IGFBP-1, IGFBP-2, and IGFBP-3. This analysis revealed expression of all three mRNAs to varying degrees by all cell lines. In contrast all primary tumours analysed expressed predominantly IGFBP-2 and IGFBP-3 and none showed any evident expression of IGFBP-1. Both NSCLC cell lines and tumours synthesise IGFBPs but the pattern of expression differs significantly between cell lines and primary tumours.
Jaques
G
G
Philipps University, Medical Centre, Marburg, F.R.G.
Kiefer
P
P
Schöneberger
H J
HJ
Wegmann
B
B
Kaiser
U
U
Brandscheid
D
D
Havemann
K
K
D015152:Blotting, Northern; D002289:Carcinoma, Non-Small-Cell Lung / Q000378:metabolism*; D002352:Carrier Proteins / Q000096:biosynthesis*; D006801:Humans; D018969:Insulin-Like Growth Factor Binding Proteins; D008175:Lung Neoplasms / Q000378:metabolism*; D008970:Molecular Weight; D012333:RNA, Messenger / Q000096:biosynthesis; D013002:Somatomedins / Q000096:biosynthesis*; D013997:Time Factors; D014407:Tumor Cells, Cultured / Q000378:metabolism
D002352:Carrier Proteins; D018969:Insulin-Like Growth Factor Binding Proteins; D012333:RNA, Messenger; D013002:Somatomedins