The application of aqueous two-phase systems to oligosaccharide synthesis by alpha-mannosidase catalysed glycosyl transfer reactions.
Aqueous two-phase systems formed from PEG and dextran have been applied to the synthesis of oligosaccharide by Jack bean alpha-mannosidase in reverse. Whilst rates of synthesis and percentage yields were similar in two-phase systems and one-phase aqueous buffer systems, a ten-fold increase in yield of product per unit of enzyme was seen. In addition, the use of aqueous two-phase systems offers potential process advantages over one-phase systems for the synthesis of oligosaccharides.
Bartlett
T J
TJ
Division of Biotechnology, School of Biological and Health Sciences, University of Westminster, London, UK.
Rastall
R A
RA
Rees
N H
NH
Adlard
M W
MW
Bucke
C
C
D002240:Carbohydrate Sequence; D003911:Dextrans / Q000737:chemistry; D005069:Evaluation Studies as Topic; D006027:Glycosides / Q000138:chemical synthesis; D007700:Kinetics; D008361:Mannosidases / Q000378:metabolism*; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000138:chemical synthesis*; D011092:Polyethylene Glycols / Q000737:chemistry; D012995:Solubility; D014867:Water / Q000737:chemistry; D043323:alpha-Mannosidase
D003911:Dextrans; D006027:Glycosides; D009844:Oligosaccharides; D014867:Water; D011092:Polyethylene Glycols; D008361:Mannosidases; D043323:alpha-Mannosidase