Elimination of immune tolerance to hepatitis virus in an animal model.
Four-day old ducklings were infected with duck hepatitis B virus to simulate perinatal transmission of hepatitis B virus. Immune tolerance was characterized as persistent viremia, antigenemia, without detection of anti-DHBs or anti-DHBc. A synthetic peptide, P125-146, mimicking one of the epitopes of the native DHBV Pre-S protein was used to cross-link to tetanus toxoid or phytohemagglutinin. These 'novel' antigens were used to immunize immune tolerant ducks, aimed at bypassing T cell tolerance. After 5 injections, though no anti-DHBV Pre-S was detected, around 50% of the immunized ducks showed seroconversion to DHBV DNA negative.
Wen
Y M
YM
Department of Microbiology, Shanghai Medical University.
Zhang
W
W
Xu
Y Y
YY
Luo
Y F
YF
Li
P Y
PY
D000595:Amino Acid Sequence; D000818:Animals; D004279:DNA, Viral; D004372:Ducks; D015611:Hepatitis B Virus, Duck / Q000276:immunology*; D006524:Hepatitis, Viral, Animal / Q000276:immunology*; D007108:Immune Tolerance / Q000276:immunology*; D008969:Molecular Sequence Data; D010835:Phytohemagglutinins; D014759:Viral Envelope Proteins; D014764:Viral Proteins / Q000235:genetics / Q000276:immunology*
D004279:DNA, Viral; D010835:Phytohemagglutinins; C077433:Pre-S protein, Duck hepatitis B virus; D014759:Viral Envelope Proteins; D014764:Viral Proteins