Human milk components cross-reacting with antibodies against bovine beta-lactoglobulin.
The human whey components cross-reacting with antibodies raised against bovine and/or equine beta-lactoglobulin were screened systematically. The milk of six women on a normal diet was collected within 72 h of confinement and whey components were fractionated by high-speed size exclusion chromatography and reversed-phase techniques. The fractions which were immunoreactive in double diffusion experiments with antisera anti-bovine and/or equine beta-lactoglobulin were subsequently purified by native PAGE and then electroblotted on Pro-blott membrane (Western blotting). Pro-blot membranes were stained in parallel with Coomassie and by immunostaining using antibodies against bovine and/or equine beta-lactoglobulin as first antibody solution. The immunoreactive bands were cut out from the membrane and N-terminally sequenced; all the immunoreactive components were clearly identified as human beta-casein or its (mainly tryptic) fragments. The strong antigenic similarity between human beta-casein and beta-lactoglobulin (bovine and equine) might be of immunological importance; it could mean that breast-fed neonates risk being sensitized to beta-lactoglobulin irrespective of the presence of cow's milk in the mother's diet.
Neuteboom
B
B
Centro Studio Alimentazione Animali del CNR Torino, Italy.
Giuffrida
M G
MG
Cantisani
A
A
Napolitano
L
L
Alessandri
A
A
Fabris
C
C
Bertino
E
E
Conti
A
A
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies; D015153:Blotting, Western; D002364:Caseins / Q000737:chemistry / Q000276:immunology*; D002417:Cattle; D002849:Chromatography, Gas; D003126:Colostrum / Q000737:chemistry / Q000276:immunology; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006736:Horses; D006801:Humans; D007782:Lactoglobulins / Q000276:immunology*; D008895:Milk, Human / Q000737:chemistry / Q000276:immunology*; D008969:Molecular Sequence Data
D000906:Antibodies; D002364:Caseins; D007782:Lactoglobulins