Prediction of the volume of distribution of 7-hydroxycoumarin in man from in vitro and ex vivo data obtained in rat.
The essential parameter to estimate the first dose size of a drug in man is the volume of distribution. For a drug that has never been used in man before, estimates of the volume of distribution can only be obtained from animals and in vitro data. The purpose of this study was to compare various approaches presented in the literature for predicting the volume of distribution at steady state (VSS) and the terminal phase volume of distribution (Vd beta) in man. A lipophilic active metabolite of coumarin, 7-hydroxycoumarin (7OHC), was selected for this investigation. This compound is extensively metabolized in both the central and peripheral compartments. Of the six methods evaluated, only an empirical allometric approach yielded a reasonable estimate of VSS. All methods underestimated VSS and none of the applicable methods were able to predict Vd beta. The reason for this discrepancy may be due to the fact that the calculation of VSS in man was done assuming elimination from the central compartment.
Ritschel
W A
WA
Division of Pharmaceutics, College of Pharmacy, University of Cincinnati Medical Center, OH 45267-004.
Johnson
R D
RD
Vachharajani
N N
NN
Hussain
A S
AS
D000818:Animals; D001798:Blood Proteins / Q000378:metabolism; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D006801:Humans; D008099:Liver / Q000378:metabolism; D008954:Models, Biological; D011485:Protein Binding; D051381:Rats; D013045:Species Specificity; D014018:Tissue Distribution; D014468:Umbelliferones / Q000493:pharmacokinetics*
D001798:Blood Proteins; D014468:Umbelliferones; C031477:7-hydroxycoumarin