title: Molecular cloning of a cDNA encoding a member of a novel cytochrome P450 family in the mollusc Lymnaea stagnalis.
abstract: We isolated a cDNA encoding a cytochrome P450 from the mollusc Lymnaea stagnalis. The mRNA is 2.1 nucleotides long and contains an open reading frame encoding a protein of 545 amino acids. A conserved home-binding domain, characteristic of cytochrome P450 proteins, is present in the deduced amino acid sequence. The Lymnaea cytochrome P450 protein shares less than 40% positional identity with any known member of the cytochrome P450 superfamily, and therefore, represents a separate family, which we propose to name CYP10. The CYP10 mRNA is shown to be uniquely and abundantly expressed in the female gonadotropic hormone producing dorsal bodies of L. stagnalis.
authors.affiliation: Department of Biology, Free University, Amsterdam, The Netherlands.
authors.forename: Y
authors.initials: Y
authors.lastname: Teunissen
authors.forename: W P
authors.initials: WP
authors.lastname: Geraerts
authors.forename: H
authors.initials: H
authors.lastname: van Heerikhuizen
authors.forename: R J
authors.initials: RJ
authors.lastname: Planta
authors.forename: J
authors.initials: J
authors.lastname: Joosse
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D003577:Cytochrome P-450 Enzyme System / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D004247:DNA / Q000235:genetics; D006418:Heme / Q000378:metabolism; D006801:Humans; D008195:Lymnaea / Q000737:chemistry*; D008969:Molecular Sequence Data; D011485:Protein Binding; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics; D014158:Transcription, Genetic
chemical_list: D012333:RNA, Messenger; D006418:Heme; D004247:DNA; D003577:Cytochrome P-450 Enzyme System