title: Epitopes for CD1a, CD1b, and CD1c antigens are differentially mapped on Langerhans cells, dermal dendritic cells, keratinocytes, and basement membrane zone in human skin.
abstract: BACKGROUND
  CD1 antigens are classified serologically into at least three groups, CD1a, CD1b, and CD1c, and many kinds of monoclonal antibodies are available for each subgroup of CD1 antigens. CD1a, CD1b, and CD1c antigens have been shown to be selectively and differentially expressed on epidermal Langerhans cells and dermal dendritic cells in normal human skin.
  
  
  OBJECTIVE
  The objective was to further delineate the localization of epitopes of CD1 antigens in human skin.
  
  
  METHODS
  We examined the immunoreactivity of 14 different CD1 antibodies (seven CD1a, five CD1b, and two CD1c antibodies) with the immunoperoxidase technique. We also studied the reactivity of NU-T2 (CD1b) antibody by immunogold electron microscopy.
  
  
  RESULTS
  The epitopes for CD1a, CD1b, and CD1c antigens were differentially mapped on epidermal Langerhans cells, dermal dendritic cells, keratinocytes, the luminal portion of eccrine gland ducts, and the basement membrane zone in human skin.
  
  
  CONCLUSIONS
  These CD1 antibodies may be useful to analyze the phenotypic alteration of immune and nonimmune cells in various skin diseases.
authors.affiliation: Department of Dermatology, University of Tokyo, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Furue
authors.forename: M
authors.initials: M
authors.lastname: Nindl
authors.forename: K
authors.initials: K
authors.lastname: Kawabe
authors.forename: K
authors.initials: K
authors.lastname: Nakamura
authors.forename: Y
authors.initials: Y
authors.lastname: Ishibashi
authors.forename: K
authors.initials: K
authors.lastname: Sagawa
mesh_terms: D015703:Antigens, CD / Q000276:immunology*; D001485:Basement Membrane / Q000276:immunology; D003713:Dendritic Cells / Q000276:immunology*; D000939:Epitopes; D006801:Humans; D007124:Immunoenzyme Techniques; D015603:Keratinocytes / Q000276:immunology*; D007801:Langerhans Cells / Q000276:immunology; D012867:Skin / Q000276:immunology*; D012871:Skin Diseases / Q000276:immunology
chemical_list: D015703:Antigens, CD; D000939:Epitopes