Rapid visual assay of cytotoxic T-cell specificity utilizing synthetic peptide induced T-cell-T-cell killing.
Synthetic peptides are widely used to define the specificity of CD8+ cytotoxic T-lymphocyte (CTL) clones. When many peptides need to be tested by the standard chromium release assay large numbers of a CTL clone are required. Specific synthetic peptide epitopes induce CTL clones to kill each other. This phenomenon can be directly visualized using an inverted microscope and forms the basis for a convenient assay, which can be performed with as few as 100 CTL per peptide and does not require radiolabelled targets.
Burrows
S R
SR
Queensland Institute of Medical Research, Bancroft Centre, Brisbane, Australia.
Suhrbier
A
A
Khanna
R
R
Moss
D J
DJ
D002478:Cells, Cultured; D002999:Clone Cells / Q000276:immunology; D003601:Cytotoxicity Tests, Immunologic / Q000379:methods*; D000939:Epitopes / Q000032:analysis*; D006801:Humans; D010455:Peptides / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
D000939:Epitopes; D010455:Peptides