Histochemistry of NADPH-diaphorase, a marker for neuronal nitric oxide synthase, in the peripheral autonomic nervous system of the mouse.
In order to identify possible sites of synthesis of nitric oxide in the peripheral nervous system, several mouse organs were investigated for the presence of NADPH-diaphorase activity. Diaphorase-positive neurons and fibers were localized in the tongue, submandibular salivary glands, gastrointestinal and biliary duct systems, lower urinary tract and pelvic ganglia. By thionin counterstaining it was found that a distinct subpopulation of neurons was labeled. The present study indicates that nitric oxide synthase may be present in intrinsic neurons of various organs, suggesting a widespread function of nitric oxide in the peripheral autonomic nervous system.
Grozdanovic
Z
Z
Department of Anatomy, Free University of Berlin, F.R.G.
Baumgarten
H G
HG
Brüning
G
G
D000594:Amino Acid Oxidoreductases / Q000032:analysis / Q000378:metabolism*; D000818:Animals; D001341:Autonomic Nervous System / Q000201:enzymology*; D015415:Biomarkers; D004064:Digestive System / Q000294:innervation; D005260:Female; D005704:Gallbladder / Q000294:innervation; D005725:Ganglia, Autonomic / Q000166:cytology / Q000201:enzymology*; D006651:Histocytochemistry; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009252:NADPH Dehydrogenase / Q000032:analysis / Q000378:metabolism*; D009474:Neurons / Q000166:cytology / Q000201:enzymology*; D019001:Nitric Oxide Synthase; D010614:Pharynx / Q000294:innervation; D013363:Submandibular Gland / Q000294:innervation; D014059:Tongue / Q000294:innervation; D014551:Urinary Tract / Q000294:innervation
D015415:Biomarkers; D019001:Nitric Oxide Synthase; D000594:Amino Acid Oxidoreductases; D009252:NADPH Dehydrogenase