title: Molecular characterization of the interleukin-1 receptor (IL-1R) on monocytes and polymorphonuclear cells.
abstract: Primary human monocytes and monocytic cells express an interleukin 1 receptor (IL-1R) which is similar in molecular weight and IL-1 binding characteristics to the IL-1R expressed on B lymphocytes (type II). Northern blot analysis of monocytic cells using a cDNA probe from the recently isolated type II IL-1R indicates that this mRNA is detectable by 4 h and accumulates for at least 24 h following treatment with IL-1R inducing drugs. The time course of induction of this mRNA is slower than that of the type I IL-1R mRNA which is also transcribed in monocytic cells but does not appear to be translated. Sequence analysis of a monocyte-derived cDNA corresponding to the type II IL-1R mRNA shows that the monocyte and B-cell mRNAs are identical. Comparison of monocyte IL-1R peptide maps with those of the type II IL-1R suggests that the two surface IL-1R are identical. This was confirmed serologically using a polyclonal antiserum raised against the type II IL-1R. Data are presented which indicate that primary human neutrophils can also be induced to express abundant type II IL-1R.
authors.affiliation: Department of Molecular Biology, Immunex Research and Development Corporation, Seattle, Washington 98101.
authors.forename: M K
authors.initials: MK
authors.lastname: Spriggs
authors.forename: P J
authors.initials: PJ
authors.lastname: Nevens
authors.forename: K
authors.initials: K
authors.lastname: Grabstein
authors.forename: S K
authors.initials: SK
authors.lastname: Dower
authors.forename: D
authors.initials: D
authors.lastname: Cosman
authors.forename: R J
authors.initials: RJ
authors.lastname: Armitage
authors.forename: C J
authors.initials: CJ
authors.lastname: McMahan
authors.forename: J E
authors.initials: JE
authors.lastname: Sims
mesh_terms: D015152:Blotting, Northern; D006801:Humans; D007375:Interleukin-1; D009000:Monocytes / Q000737:chemistry*; D009504:Neutrophils / Q000737:chemistry*; D012333:RNA, Messenger / Q000235:genetics; D011971:Receptors, Immunologic / Q000737:chemistry* / Q000235:genetics; D017472:Receptors, Interleukin-1; D013256:Steroids / Q000494:pharmacology; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D007375:Interleukin-1; D012333:RNA, Messenger; D011971:Receptors, Immunologic; D017472:Receptors, Interleukin-1; D013256:Steroids