title: Evolutionary conservation of the 14-3-3 protein.
abstract: The novel family of 14-3-3 proteins may be involved in the regulation of neuronal activity. During our search for proteins coordinately expressed with the prohormone proopiomelanocortin in the melanotrope cells of the Xenopus intermediate pituitary gland, we cloned and sequenced a pituitary cDNA encoding a Xenopus 14-3-3 protein. Alignment of the Xenopus protein with known mammalian, Drosophila and plant 14-3-3 polypeptide and with a mammalian protein kinase C inhibitor protein revealed that the neuron-specific 14-3-3-related proteins are highly conserved (60-88%) throughout eukaryotic evolution.
authors.affiliation: Department of Animal Physiology, University of Nijmegen, Toernooiveld, The Netherlands.
authors.forename: G J
authors.initials: GJ
authors.lastname: Martens
authors.forename: P A
authors.initials: PA
authors.lastname: Piosik
authors.forename: E H
authors.initials: EH
authors.lastname: Danen
mesh_terms: D048948:14-3-3 Proteins; D000595:Amino Acid Sequence; D000818:Animals; D005075:Biological Evolution; D015152:Blotting, Northern; D002417:Cattle; D004247:DNA / Q000235:genetics; D004330:Drosophila / Q000235:genetics; D015723:Gene Library; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics*; D010902:Pituitary Gland / Q000502:physiology*; D010944:Plants / Q000235:genetics; D011333:Pro-Opiomelanocortin / Q000235:genetics; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012689:Sequence Homology, Nucleic Acid; D012756:Sheep; D014446:Tyrosine 3-Monooxygenase; D014982:Xenopus laevis
chemical_list: D048948:14-3-3 Proteins; D009419:Nerve Tissue Proteins; D012313:RNA; D011333:Pro-Opiomelanocortin; D004247:DNA; D014446:Tyrosine 3-Monooxygenase