Specific detection of c-erbB-2 mRNA expression in gastric cancers by the polymerase chain reaction following reverse transcription.
The expression of the c-erbB-2 mRNA in human embryonic lung fibroblasts, a gastric cancer cell line, mature placenta, and 25 gastric cancer tissues was examined by using the polymerase chain reaction following reverse transcription. This technique can be used to examine c-erbB-2 mRNA expression in a small endoscopic biopsy specimen before surgery.
Ninomiya
I
I
Department of Surgery II, School of Medicine, Kanazawa University, Japan.
Endo
Y
Y
Yonemura
Y
Y
Noguchi
M
M
Fushida
S
S
Nakai
M
M
Takamura
H
H
Harada
F
F
Suzuki
T
T
Miyazaki
I
I
D000199:Actins / Q000235:genetics; D001483:Base Sequence; D015139:Blotting, Southern; D002460:Cell Line; D006801:Humans; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D016376:Oligonucleotides, Antisense; D016133:Polymerase Chain Reaction / Q000379:methods; D011505:Protein-Tyrosine Kinases / Q000235:genetics; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D011519:Proto-Oncogenes; D012333:RNA, Messenger / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D012194:RNA-Directed DNA Polymerase; D018719:Erb-b2 Receptor Tyrosine Kinases; D013274:Stomach Neoplasms / Q000235:genetics*
D000199:Actins; D009838:Oligodeoxyribonucleotides; D016376:Oligonucleotides, Antisense; D011505:Protein-Tyrosine Kinases; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D012194:RNA-Directed DNA Polymerase; D018719:Erb-b2 Receptor Tyrosine Kinases