title: Ultrasonic prediction of carcass merit in beef cattle: evaluation of technician effects on ultrasonic estimates of carcass fat thickness and longissimus muscle area.
abstract: The objective of this study was to determine technician effects of live animal ultrasonic estimates of fat thickness (FTU) and longissimus muscle area (LMAU). Steers (n = 36) representing four breed-types (Brown Swiss, Average Zebu-cross Mexican, Corriente Mexican, and typical British crossbred) of commercial slaughter cattle were isonified to estimate accuracy and repeatability of fat thickness (FT) and longissimus muscle area (LMA) measurements by two experienced technicians. Repeated measures of FTU and LMAU were taken by technicians on two consecutive days with an Aloka 500V ultrasound unit equipped with a 3.5-MHz, 172-mm scanning width, linear-array transducer. Ultrasonic estimates of fat thickness and LMAU were taken at the 12th and 13th rib interface 48 h before slaughter; carcass fat thickness (FTC) and longissimus muscle area (LMAC) were measured 48 h postmortem. Means for FTU, FTC, LMAU, and LMAC were .91 +/- .36 cm, .82 +/- .40 cm, 70.7 +/- 9.43 cm2, and 72.4 +/- 8.9 cm2, respectively. Ultrasound and carcass measures of FT and LMA were different (P less than .01) among breed-types but were not different (P greater than .10) between technicians or for technician x breed-type interactions. Pooled simple correlation coefficients (P less than .01) were .87 and .86 between FTU and FTC and .76 and .82 between LMAU and LMAC for Technicians 1 and 2, respectively. Repeatabilities estimated by intraclass correlation methods were .91 +/- .03 and .81 +/- .06 for images repeated over 2 d and .95 +/- .02 and .83 +/- .05 for images repeated by two technicians for FT and LMA, respectively.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Animal Science, Texas Tech University, Lubbock 79409-2141.
authors.forename: T L
authors.initials: TL
authors.lastname: Perkins
authors.forename: R D
authors.initials: RD
authors.lastname: Green
authors.forename: K E
authors.initials: KE
authors.lastname: Hamlin
authors.forename: H H
authors.initials: HH
authors.lastname: Shepard
authors.forename: M F
authors.initials: MF
authors.lastname: Miller
mesh_terms: D000273:Adipose Tissue / Q000000981:diagnostic imaging*; D000818:Animals; D001947:Breeding; D002417:Cattle / Q000033:anatomy & histology*; D005069:Evaluation Studies as Topic; D008297:Male; D008460:Meat / Q000592:standards*; D009132:Muscles / Q000000981:diagnostic imaging*; D015588:Observer Variation; D011237:Predictive Value of Tests; D015203:Reproducibility of Results; D014463:Ultrasonography