Analysis for cerebrospinal fluid proteins by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
Cerebrospinal fluid (CSF) proteins with molecular masses of < 150,000 Da were identified by immunoblotting after two kinds of nonreducing sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). With PAGE 1 (17-27% gradient gel), CSF proteins were clearly separated into seven to nine bands with molecular masses of 3000-67,000 Da; seven bands were identified as beta 2-microglobulin, lysozyme, prealbumin, free kappa and lambda chain, apolipoprotein A-I, glycoproteins, and albumin by immunoblotting. With PAGE 2 (10-20% gradient gel), proteins were clearly separated into 11-16 bands with molecular masses of 15,000-150,000 Da; 11 were identified as prealbumin, free kappa and lambda chain, apolipoprotein A-I, glycoproteins, albumin, alpha 1-antitrypsin, transferrin (separated into two bands), immunoglobulin fragments, haptoglobin, and IgG. We analyzed CSF samples collected from 81 patients with cerebrospinal signs by these SDS-PAGE methods and observed prominent bands in some cases.
Mashige
F
F
Department of Laboratory Medicine, Faculty of Medicine, University of Tokyo, Japan.
Shimizu
T
T
Iijima
S
S
Ohkubo
A
A
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000134:cerebrospinal fluid; D001927:Brain Diseases / Q000134:cerebrospinal fluid; D002556:Cerebrospinal Fluid Proteins / Q000032:analysis*; D002648:Child; D002675:Child, Preschool; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D005260:Female; D006454:Hemoglobins / Q000134:cerebrospinal fluid; D006801:Humans; D015151:Immunoblotting; D007938:Leukemia / Q000134:cerebrospinal fluid; D008297:Male; D008875:Middle Aged; D008970:Molecular Weight; D009113:Muramidase / Q000134:cerebrospinal fluid; D012016:Reference Values; D001613:beta 2-Microglobulin / Q000134:cerebrospinal fluid
D002556:Cerebrospinal Fluid Proteins; D006454:Hemoglobins; D001613:beta 2-Microglobulin; D009113:Muramidase