Atrial and brain natriuretic peptides enhance dopamine accumulation in cultured rat hypothalamic cells including dopaminergic neurons.
Dopamine accumulation in hypothalamic cells by atrial natriuretic peptide (ANP) and brain natriuretic peptide (BNP) was analyzed using newborn rat hypothalamic cells in culture. Both ANP and BNP caused a dose-dependent increase in [3H]-dopamine accumulation in the cells. ANP increased [3H]-dopamine accumulation significantly within 20 min. The effects of ANP and BNP on dopamine accumulation paralleled an increase in intracellular cGMP concentration. (Bu)2-cGMP and sodium nitroprusside, a stimulator of the soluble form of guanylate cyclase, also enhanced [3H]-dopamine accumulation. ANP had no effect on efflux of [3H] radioactivity after [3H]-dopamine uptake. These results suggest that a change in cGMP is one of the intermediate steps in dopamine accumulation in hypothalamic cells by ANP and BNP.
Kadowaki
K
K
Department of Obstetrics and Gynecology, Osaka University Medical School, Japan.
Hirota
K
K
Koike
K
K
Ohmichi
M
M
Miyake
A
A
Tanizawa
O
O
D000818:Animals; D009320:Atrial Natriuretic Factor / Q000494:pharmacology*; D002478:Cells, Cultured; D004298:Dopamine / Q000378:metabolism*; D007031:Hypothalamus / Q000166:cytology / Q000378:metabolism*; D020097:Natriuretic Peptide, Brain; D009419:Nerve Tissue Proteins / Q000494:pharmacology*; D009474:Neurons / Q000378:metabolism*; D009599:Nitroprusside / Q000494:pharmacology; D051381:Rats; D013997:Time Factors
D009419:Nerve Tissue Proteins; D020097:Natriuretic Peptide, Brain; D009599:Nitroprusside; D009320:Atrial Natriuretic Factor; D004298:Dopamine