Capillary gas-liquid chromatographic separation of bile alcohols.
Gas-liquid chromatographic separation of C23, C24, C25, C26, and C27 bile alcohols with either 3 alpha, 7 alpha-dihydroxylated or 3 alpha, 7 alpha, 12 alpha-trihydroxylated ring system on two capillary columns, CP-Sil-19 CB and CP-Sil-5 CB, is described. Bile alcohols with two ring hydroxyl groups at 3 alpha- and 7 alpha-positions consistently showed larger retention times on CP-Sil-19 CB columns and smaller retention times on CP-Sil-5 CB columns than the corresponding bile alcohols with three ring hydroxyl groups at 3 alpha-, 7 alpha-, and 12 alpha-positions. Resolutions of all bile alcohols were better on CP-Sil-19 CB columns; however, we hope that the gas-liquid chromatographic characteristics on the two columns will be useful for better identification of bile alcohols in biological fluids.
Batta
A K
AK
Department of Medicine, University of Medicine and Dentistry of New Jersey, New Jersey Medical School, Newark 07103.
Aggarwal
S K
SK
Mirchandani
R
R
Shefer
S
S
Salen
G
G
D002777:Cholestanols / Q000737:chemistry / Q000302:isolation & purification* / Q000652:urine; D002849:Chromatography, Gas / Q000379:methods*; D006801:Humans; D015394:Molecular Structure; D014973:Xanthomatosis / Q000652:urine
D002777:Cholestanols