title: Ultracytochemical demonstration of Ca(++)-ATPase activity in the rat cardiac muscle.
abstract: Ca(++)-activated adenosine triphosphatase (Ca(++)-ATPase) was investigated in the rat cardiac muscle at neutral pH using tricine buffer. Reaction products indicating Ca(++)-ATPase activity were localized on the myocardial sarcolemma, sarcoplasmic reticulum, myofilaments, luminal and abluminal surfaces of capillary endothelium plasmatic membrane. The verification of the main enzymatic characteristics of Ca(++)-ATPase localization activity using this cytochemical procedure is under discussion.
authors.affiliation: Department of Pathological Anatomy, Kiev Medical Institute, USSR.
authors.forename: V S
authors.initials: VS
authors.lastname: Zinchuk
authors.forename: A V
authors.initials: AV
authors.lastname: Bulavka
mesh_terms: D008841:Actin Cytoskeleton / Q000201:enzymology / Q000648:ultrastructure; D000818:Animals; D000252:Calcium-Transporting ATPases / Q000032:analysis*; D004730:Endothelium, Vascular / Q000201:enzymology / Q000648:ultrastructure; D006651:Histocytochemistry; D008297:Male; D008854:Microscopy, Electron; D009206:Myocardium / Q000166:cytology / Q000201:enzymology* / Q000648:ultrastructure; D051381:Rats; D012508:Sarcolemma / Q000201:enzymology / Q000648:ultrastructure; D012519:Sarcoplasmic Reticulum / Q000201:enzymology / Q000648:ultrastructure
chemical_list: D000252:Calcium-Transporting ATPases