Detection of potyviruses with antisera to synthetic peptides.
Eight peptides corresponding to conserved regions of the coat protein of potyviruses were synthesized. All the peptides were recognized by anti-virus or anti-core-virus. Antisera raised to the synthetic peptides were tested with purified viruses and viral antigens present in plant sap. In many cases, the extent of cross-reactivity between different potyviruses was not correlated with the degree of sequence homology between the peptide used for immunization and the corresponding region in the coat protein of the potyvirus tested. An antiserum raised to a peptide of 18 residues containing a highly conserved region was found to react with all seven potyviruses tested.
Joisson
C
C
Laboratoire d'Immunochimie, Institut de Biologie moléculaire et cellulaire, Strasbourg, France.
Dubs
M C
MC
Briand
J P
JP
Van Regenmortel
M H
MH
D000595:Amino Acid Sequence; D000818:Animals; D000937:Antigen-Antibody Reactions; D002645:Chickens; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000737:chemistry / Q000276:immunology; D007106:Immune Sera / Q000737:chemistry*; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000276:immunology*; D010935:Plant Diseases / Q000382:microbiology; D010936:Plant Extracts / Q000276:immunology; D010942:Plant Viruses / Q000276:immunology / Q000302:isolation & purification*; D011817:Rabbits; D014758:Viral Core Proteins / Q000276:immunology
D000939:Epitopes; D007106:Immune Sera; D010446:Peptide Fragments; D010936:Plant Extracts; D014758:Viral Core Proteins