Determination of cefcanel in plasma and urine by high-performance liquid chromatography using coupled columns, after administration of the new cephalosporin prodrug cefcanel daloxate hydrochloride.
A rapid and sensitive high-performance liquid chromatographic method has been developed for the determination in plasma and urine of the new cephalosporin cefcanel. The method involves a simple deproteinizing step followed by separation on a coupled-column chromatographic system with ultraviolet detection. Limits of quantification were 0.2 microM for plasma samples and 2 microM for urine samples. The method has been used for the determination of cefcanel in various clinical studies.
Lanbeck-Vallén
K
K
Department of Bioanalysis, Astra Arcus AB, Södertäje, Sweden.
Carlqvist
J
J
D002437:Cefazolin / Q000031:analogs & derivatives* / Q000097:blood / Q000652:urine; D002511:Cephalosporins / Q000008:administration & dosage* / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D011355:Prodrugs / Q000008:administration & dosage*; D013048:Specimen Handling
D002511:Cephalosporins; D011355:Prodrugs; C022605:cefcanel; C045380:cefcanel daloxate; D002437:Cefazolin