title: A monoclonal antibody, raised against mammalian centrosomes and screened by recognition of plant microtubule organizing centers, identifies a pericentriolar component in different cell types.
abstract: We have used monoclonal antibodies raised against isolated native calf thymus centrosomes to probe the structure and composition of the pericentriolar material. To distinguish prospective antibodies as specific to conserved elements of this material, we screened clones by their identification of microtubule organizing centers (MTOCs) in different animal and plant cells. Among the clonal antibodies that reacted with MTOCs in both plant and mammalian cells, we describe one (mAb 6C6) that was found to immunostain centrosomes in a variety of bovine and human cells. In cycling cells this signal persisted through the entire cell cycle. Microscopy showed that the mAb 6C6 antigen was a component of the pericentriolar material and this was confirmed by biochemical analysis of centrosomes. Using immunoblot analysis of protein fractions derived from purified components of centrosomes, we have characterized the mAb 6C6 antigen as a 180 kDa polypeptide. We conclude that we have identified a protein component permanently associated with the pericentriolar material. Surprisingly, monoclonal antibody 6C6 also stained other mitotic organelles in mammalian cells, in a cell-cycle-dependent manner. During prometaphase and metaphase the antibody stained both centrosomes and kinetochores. At the onset of anaphase the kinetochore-specific staining dissociated from chromosomes and was subsequently redistributed onto a newly characterized organelle, the telophase disc while the centrosomal stain remained intact. It is not known if the 180 kDa centrosomal protein itself redistributes during mitosis, or if the pattern observed represents other antigens with shared epitopes. The pericentriolar material is thought to be composed of conserved elements, which appeared very early during the evolution of eukaryotes. Our results strongly suggest that mAb 6C6 identifies one of these elements.
authors.affiliation: Département de Biologie Moléculaire et Structurale, INSERM U244, Centre d'Etudes Nucléaires, Grenoble, France.
authors.forename: V
authors.initials: V
authors.lastname: Chevrier
authors.forename: S
authors.initials: S
authors.lastname: Komesli
authors.forename: A C
authors.initials: AC
authors.lastname: Schmit
authors.forename: M
authors.initials: M
authors.lastname: Vantard
authors.forename: A M
authors.initials: AM
authors.lastname: Lambert
authors.forename: D
authors.initials: D
authors.lastname: Job
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D002417:Cattle; D002460:Cell Line; D002502:Centrioles / Q000737:chemistry / Q000276:immunology*; D000939:Epitopes; D006367:HeLa Cells; D006801:Humans; D015151:Immunoblotting; D016253:Microscopy, Immunoelectron; D008868:Microtubule Proteins / Q000032:analysis / Q000276:immunology; D008870:Microtubules / Q000276:immunology*; D008941:Spindle Apparatus / Q000276:immunology; D013950:Thymus Gland / Q000737:chemistry / Q000648:ultrastructure; D003313:Zea mays / Q000737:chemistry / Q000276:immunology* / Q000648:ultrastructure
chemical_list: D000911:Antibodies, Monoclonal; D000939:Epitopes; D008868:Microtubule Proteins