Comparison of the biodistribution of 75Se- and 131I-labelled monoclonal antibodies in nude mice.
A monoclonal antibody, C-215, against colon cancer, was internally labelled with [75Se]methionine. The biodistribution was studied in tumour-bearing nude mice and compared with the biodistribution of [131I]C-215. The tissue uptake was divided into three parts: antibody bound to the antigen, antibody in the extracellular space and uptake of the released radionuclide. [75Se]C-215 showed a greater amount of antigen-bound antibody in the tumour, but also a greater unspecific uptake both in tumour and normal tissue.
Grétarsdóttir
J
J
Department of Radiation Physics, University of Göteborg, Sahlgrenska Hospital, Sweden.
Aronsson
E F
EF
Jacobsson
L
L
Hafsteinsdóttir
O
O
Holmberg
S
S
Hafström
L
L
Lindegren
S
S
Karlsson
B
B
Lindholm
L
L
Mattsson
S
S
D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism*; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000032:analysis / Q000276:immunology; D002460:Cell Line; D003110:Colonic Neoplasms / Q000000981:diagnostic imaging*; D006801:Humans; D007074:Immunoglobulin G / Q000145:classification / Q000378:metabolism; D007457:Iodine Radioisotopes; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D011877:Radionuclide Imaging; D012644:Selenium Radioisotopes; D013997:Time Factors; D014018:Tissue Distribution; D014183:Transplantation, Heterologous
D000911:Antibodies, Monoclonal; D015295:Antigens, Tumor-Associated, Carbohydrate; D007074:Immunoglobulin G; D007457:Iodine Radioisotopes; D012644:Selenium Radioisotopes