Molecular characterization of the interleukin-1 receptor (IL-1R) on monocytes and polymorphonuclear cells.
Primary human monocytes and monocytic cells express an interleukin 1 receptor (IL-1R) which is similar in molecular weight and IL-1 binding characteristics to the IL-1R expressed on B lymphocytes (type II). Northern blot analysis of monocytic cells using a cDNA probe from the recently isolated type II IL-1R indicates that this mRNA is detectable by 4 h and accumulates for at least 24 h following treatment with IL-1R inducing drugs. The time course of induction of this mRNA is slower than that of the type I IL-1R mRNA which is also transcribed in monocytic cells but does not appear to be translated. Sequence analysis of a monocyte-derived cDNA corresponding to the type II IL-1R mRNA shows that the monocyte and B-cell mRNAs are identical. Comparison of monocyte IL-1R peptide maps with those of the type II IL-1R suggests that the two surface IL-1R are identical. This was confirmed serologically using a polyclonal antiserum raised against the type II IL-1R. Data are presented which indicate that primary human neutrophils can also be induced to express abundant type II IL-1R.
Spriggs
M K
MK
Department of Molecular Biology, Immunex Research and Development Corporation, Seattle, Washington 98101.
Nevens
P J
PJ
Grabstein
K
K
Dower
S K
SK
Cosman
D
D
Armitage
R J
RJ
McMahan
C J
CJ
Sims
J E
JE
D015152:Blotting, Northern; D006801:Humans; D007375:Interleukin-1; D009000:Monocytes / Q000737:chemistry*; D009504:Neutrophils / Q000737:chemistry*; D012333:RNA, Messenger / Q000235:genetics; D011971:Receptors, Immunologic / Q000737:chemistry* / Q000235:genetics; D017472:Receptors, Interleukin-1; D013256:Steroids / Q000494:pharmacology; D015854:Up-Regulation / Q000187:drug effects
D007375:Interleukin-1; D012333:RNA, Messenger; D011971:Receptors, Immunologic; D017472:Receptors, Interleukin-1; D013256:Steroids