Acquisition of apparently intact and unmodified lipopolysaccharides from Escherichia coli by Bdellovibrio bacteriovorus.
The ability of Bdellovibrio bacteriovorus to relocalize the OmpF major outer membrane porins from its Escherichia coli prey to its own outer membranes is diminished in prey expressing smooth lipopolysaccharide (S-LPS). Since porins exist in the membrane complexed with LPS, we examined the LPS associated with relocalized porin to determine whether it had been acquired intact, mixed or replaced with Bdellovibrio LPS, or derivatized by the bdellovibrios. The relocalized trimers were found associated with the same LPS originally bound to them in the E. coli. The bulk-phase LPS from bdellovibrios grown on various chemotypes of rough prey was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis to determine whether more than the trimer-bound LPS was acquired by the bdellovibrios. This analysis revealed bands of Bdellovibrio LPS matching the LPS chemotype of the prey. One or two other bands were identical in migration to the LPS of prey-independent mutants of B. bacteriovorus and represented bdellovibrio-synthesized LPS. The LPS of bdellovibrios grown on prey with radiolabeled lipid A showed radioactivity only in gel band positions identical with those of the prey's LPS. The amount of this prey-derived LPS was shown by enzyme-linked immunosorbent assay to reach a constant value during the purification of the bdellovibrios, and it represented approximately 25% of the total Bdellovibrio LPS. Immunoelectron microscopy confirmed the presence of prey-derived LPS on the cell surface of bdellovibrios, and no evidence could be found for bdellovibrio-induced modifications of the relocalized prey LPS.
Stein
M A
MA
Department of Microbiology, Immunology, and Parasitology, Louisiana State University Medical Center, New Orleans 70112-1393.
McAllister
S A
SA
Torian
B E
BE
Diedrich
D L
DL
D001425:Bacterial Outer Membrane Proteins / Q000276:immunology / Q000378:metabolism; D001501:Bdellovibrio / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes; D004926:Escherichia coli / Q000378:metabolism*; D005690:Galactose / Q000378:metabolism; D014644:Genetic Variation; D005944:Glucosamine / Q000378:metabolism; D006790:Host-Parasite Interactions; D008050:Lipid A / Q000378:metabolism; D008070:Lipopolysaccharides / Q000302:isolation & purification / Q000378:metabolism*; D016253:Microscopy, Immunoelectron; D019081:O Antigens; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis / Q000378:metabolism*
D001425:Bacterial Outer Membrane Proteins; D000939:Epitopes; D008050:Lipid A; D008070:Lipopolysaccharides; D019081:O Antigens; D011135:Polysaccharides, Bacterial; D005944:Glucosamine; D005690:Galactose