title: In-vitro fertilization in cases with severe sperm defect: use of a swim-across technique and medium supplemented with follicular fluid.
abstract: The purpose of this study was to evaluate a new method of in-vitro fertilization (IVF) in patients with severe sperm defects. Unlike the conventional swim-up method, spermatozoa and oocytes are placed in opposite corners of the bottom of the incubation dish so that sperm swimming is horizontal instead of vertical. Another difference between the swim-across and swim-up techniques is that the incubation medium is supplemented with 20% follicular fluid. After a randomized series (protocol I) of 15 IVF attempts had demonstrated that swim-across was more effective than swim-up in terms of fertilization and cleavage, we began a second series (protocol II) using only swim-across. A total of 124 couples with motile sperm counts less than 1 x 10(6) spermatozoa/ml of semen were included in protocol II. Clinical parameters (age, tubal damage) and number of recovered oocytes were recorded and compared in patients who did (group A: n = 94) and did not (group B: n = 74) achieve fertilization. In group A the fertilization rate was 36.7% and, out of the 94 transfers that were made, there were 21 clinical pregnancies and 12 full-term pregnancies with 16 live births. The number of oocytes collected (12 versus 7.7, P less than 0.001) and the incidence of tubal damage (50% versus 24.3%, P less than 0.001) was significantly higher in group A than in group B. Using logistic regression analysis, we showed a significant correlation between fertilization and progressive motility, percentage normal spermatozoa, number of recovered oocytes and tubal damage.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Institut de Médecine de la Reproduction, Marseille, France.
authors.forename: C
authors.initials: C
authors.lastname: Giorgetti
authors.forename: E
authors.initials: E
authors.lastname: Hans
authors.forename: J L
authors.initials: JL
authors.lastname: Spach
authors.forename: P
authors.initials: P
authors.lastname: Auquier
authors.forename: R
authors.initials: R
authors.lastname: Roulier
mesh_terms: D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D015571:Follicular Fluid; D006801:Humans; D007248:Infertility, Male / Q000503:physiopathology / Q000628:therapy*; D008297:Male; D011247:Pregnancy; D012044:Regression Analysis; D013076:Sperm Count; D013081:Sperm Motility; D013094:Spermatozoa / Q000502:physiology*