Nucleotide sequence of the R721 shufflon.
The shufflon is a DNA region that undergoes complex rearrangement mediated by the product of a putative site-specific recombinase gene, rci. The DNA sequences of the shufflon region and the rci gene of IncI2 plasmid R721 were determined. The R721 shufflon consists of three invertible DNA segments that are homologous to the shufflon segments found in IncI1 plasmid R64. Structural analysis of open reading frames indicated that the R721 shufflon possibly functions as a biological switch for selecting one of the six pilV genes in which the N-terminal region is constant and the C-terminal region is variable. The R721 rci gene was shown to encode a basic protein of 374 amino acid residues.
Kim
S R
SR
Department of Biology, Tokyo Metropolitan University, Japan.
Komano
T
T
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D002874:Chromosome Mapping; D004254:DNA Nucleotidyltransferases / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics*; D015321:Gene Rearrangement / Q000235:genetics*; D005798:Genes, Bacterial / Q000235:genetics; D019426:Integrases; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics*; D045522:Recombinases; D011995:Recombination, Genetic / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
D001426:Bacterial Proteins; D004269:DNA, Bacterial; C077573:PilV protein, Bacteria; D045522:Recombinases; D004254:DNA Nucleotidyltransferases; D019426:Integrases; C077570:Site-specific recombinase; C115006:integron integrase IntI1