title: Separation of reduced and oxidized glutathione in a pharmaceutical preparation by ion-interaction reversed-phase HPLC.
abstract: A new method is presented for the simultaneous determination of reduced and oxidized glutathione by ion-interaction reversed-phase HPLC with octylamine orthophosphate as the interaction reagent, 5-microns Spherisorb ODS-2 as the stationary phase and UV detection at 230 nm. Lower limits of detection of 2.50 micrograms ml-1 have been achieved for both the oxidized and the reduced forms. The method has been applied in the analysis of a commercial pharmaceutical preparation.
authors.affiliation: Dipartimento di Chimica Analitica, Università di Torino, Italy.
authors.forename: M C
authors.initials: MC
authors.lastname: Gennaro
authors.forename: C
authors.initials: C
authors.lastname: Abrigo
mesh_terms: D000975:Antioxidants / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005978:Glutathione / Q000032:analysis*; D010084:Oxidation-Reduction
chemical_list: D000975:Antioxidants; D005978:Glutathione