title: Ion trap detector--capillary gas chromatography of valproic acid and its mono-unsaturated metabolites in serum using methyl ester derivatives.
abstract: A quantitative method was developed for valproic acid and five of its mono-unsaturated metabolites using capillary gas chromatography-mass spectrometry with selected ion monitoring. The method was applied to serum and all metabolites were measured in a single run. Methyl esters were synthesized as the derivatives suitable for gas chromatography. Calibration curves were found to be linear and the sensitivities in the order of 0.1 micrograms ml-1. Patients' data are presented. By this method it is possible to separate the stereoisomers of 2-n-propyl-2-pentenoic acid and of 2-n-propyl-3-pentenoic acid.
authors.affiliation: Istituto di Chimica Farmaceutica e Tossicologica, Università degli Studi di Parma, Italy.
authors.forename: E
authors.initials: E
authors.lastname: Gaetani
authors.forename: C F
authors.initials: CF
authors.lastname: Laureri
authors.forename: M
authors.initials: M
authors.lastname: Vitto
mesh_terms: D002138:Calibration; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D008745:Methylation; D013237:Stereoisomerism; D014635:Valproic Acid / Q000097:blood* / Q000378:metabolism
chemical_list: D014635:Valproic Acid