Gene for the major antigenic structural protein (p100) of human herpesvirus 6.
A human herpesvirus 6 (HHV-6) structural protein of 100 kDa (p100) is the polypeptide most frequently and intensively reactive in immunoblotting analyses with human sera on HHV-6-infected cells or partially purified virions. The gene for p100 was identified by screening a bacteriophage lambda library with monospecific rabbit antisera. The gene codes for a polypeptide of 870 amino acids with a calculated molecular size of 97 kDa. Its amino-terminal third is weakly homologous to the immunogenic basic matrix phosphoprotein pp150 of human cytomegalovirus. Five fragments representing more than 93% of HHV-6 p100 were prokaryotically expressed. The antigenic epitopes of p100 were preliminary mapped by immunoblotting with human sera. They are located within the carboxy-terminal part which is neither homologous nor cross-reactive to pp150 of human cytomegalovirus. Availability of the gene for the immunodominant structural protein should provide tools for studies of pathogenesis by HHV-6.
Neipel
F
F
Institut für klinische und molekulare Virologie, Universität Erlangen-Nürnberg, Germany.
Ellinger
K
K
Fleckenstein
B
B
D000595:Amino Acid Sequence; D001483:Base Sequence; D002874:Chromosome Mapping; D000939:Epitopes / Q000276:immunology*; D004926:Escherichia coli / Q000235:genetics; D015723:Gene Library; D015654:Herpesvirus 6, Human / Q000235:genetics* / Q000276:immunology; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D010750:Phosphoproteins; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D014763:Viral Matrix Proteins; D014764:Viral Proteins / Q000097:blood / Q000235:genetics* / Q000276:immunology
D000939:Epitopes; D010446:Peptide Fragments; D010750:Phosphoproteins; D011993:Recombinant Fusion Proteins; D014763:Viral Matrix Proteins; D014764:Viral Proteins; C056367:pp150 protein, Cytomegalovirus; C074553:p100 protein, Human herpesvirus 6