title: Distinctive pattern of infection and replication of HIV1 strains in blood-derived macrophages.
abstract: The macrophage-tropic virus HIV1-PAR, isolated from cerebrospinal fluid of HIV1-seropositive man, induced cytopathic effect accompanied by different magnitude of the virus production in blood-derived macrophages (BDM) obtained from different donors. HIV1-PAR-specific RNA was detected by in situ hybridization in 15 and 66% of BDM producing low and high levels of virus, respectively. In contrast with HIV1-PAR, infection of BDM with two laboratory strains adapted to T-cell lines, HIV1-LAV prototype and HIV1-NDK, a Zairian virus that is highly cytopathic for T-lymphocytes, resulted in a low production of HIV1 p24gag in culture fluid. Expression of HIV1-LAV and HIV1-NDK RNA was detected by in situ hybridization in a maximum of 1% of macrophages. Only HIV1-NDK, and not HIV1-LAV, induced ultrastructural alterations in BDM. In contrast with a striking difference in the production of macrophage-tropic and T-lymphotropic viruses, no significant differences were found in the proportion of macrophages containing retrotranscribed genomes of HIV1-. HIV1 DNA was detected by in situ hybridization in 93, 100, and 80% of macrophages infected with HIV1-PAR, HIV1-LAV, and HIV1-NDK, respectively. A higher level of HIV1 DNA was detected by polymerase chain reaction in the BDM infected with HIV1-PAR than in that infected with HIV1-LAV and HIV1-NDK. The results indicate that both macrophage-tropic as well as T-lymphotropic viruses can enter and retrotranscribe their genomes in a vast majority of macrophages.
authors.affiliation: Unité de Recherches INSERM U322 sur les Rétrovirus et Maladies Associées, Marseille, France.
authors.forename: H
authors.initials: H
authors.lastname: Schmidtmayerova
authors.forename: C
authors.initials: C
authors.lastname: Bolmont
authors.forename: S
authors.initials: S
authors.lastname: Baghdiguian
authors.forename: I
authors.initials: I
authors.lastname: Hirsch
authors.forename: J C
authors.initials: JC
authors.lastname: Chermann
mesh_terms: D004279:DNA, Viral / Q000032:analysis; D005312:Fetal Blood; D016655:HIV Core Protein p24 / Q000032:analysis; D054303:HIV Reverse Transcriptase; D015497:HIV-1 / Q000201:enzymology / Q000235:genetics / Q000502:physiology* / Q000648:ultrastructure; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000382:microbiology; D008214:Lymphocytes / Q000382:microbiology; D008264:Macrophages / Q000382:microbiology* / Q000648:ultrastructure; D008854:Microscopy, Electron; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D011533:Proviruses / Q000235:genetics; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D014779:Virus Replication
chemical_list: D004279:DNA, Viral; D016655:HIV Core Protein p24; D054303:HIV Reverse Transcriptase; D012194:RNA-Directed DNA Polymerase