Multiple Ca2+ signaling pathways converge on CaM kinase in PC12 cells.
The role of multifunctional Ca2+/calmodulin-dependent protein kinase (CaM kinase) in mediating various Ca2+ signaling pathways was examined in PC12 cells. Conversion of the kinase to a Ca(2+)-independent form was used to monitor which neurotransmitters activate the enzyme in situ. CaM kinase responds to Ca2+ influx elicited by ligand-gated Ca2+ channels for ATP and acetylcholine. It also responds to Ca2+ mobilization of IP3-sensitive stores elicited by phospholipase C-linked receptors for ATP and acetylcholine as well as by caffeine. CaM kinase mediates the actions of many neurotransmitters and Ca2+ signaling pathways.
MacNicol
M
M
Department of Pharmacology, Stanford University School of Medicine, CA 94305-5332.
Schulman
H
H
D000109:Acetylcholine / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D002450:Cell Communication / Q000502:physiology*; D004789:Enzyme Activation; D007473:Ion Channels / Q000378:metabolism*; D009116:Muscarine / Q000378:metabolism; D016716:PC12 Cells; D011494:Protein Kinases / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000378:metabolism*; D010738:Type C Phospholipases / Q000378:metabolism
D007473:Ion Channels; D011956:Receptors, Cell Surface; D009116:Muscarine; D000255:Adenosine Triphosphate; D011494:Protein Kinases; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D010738:Type C Phospholipases; D000109:Acetylcholine; D002118:Calcium