title: Colony morphotype on Sabouraud-triphenyltetrazolium agar: a simple and inexpensive method for Candida subspecies discrimination.
abstract: A new method of Candida subspecies discrimination on Sabouraud-triphenyltetrazolium agar is reported. Five hundred sixty-two strains of Candida and Torulopsis glabrata, previously identified by conventional mycological methods, were studied. Each strain received a three-letter code and a number based on its colonial morphology. Sixteen morphotypes were found for Candida albicans, 6 were found for Candida parapsilosis, 4 were found for both Candida guilliermondii and Candida krusei, and 12 were found for Candida tropicalis. None of the 56 T. glabrata strains studied grew on this agar. A reproducibility of 95% was found for C. albicans. The simplicity and low cost could make this method useful for typing Candida spp.
authors.affiliation: Departamento de Microbiología e Inmunología, Facultad de Medicina y Odontología, Universidad del País Vasco, Bilbao, Spain.
authors.forename: G
authors.initials: G
authors.lastname: Quindós
authors.forename: M
authors.initials: M
authors.lastname: Fernández-Rodríguez
authors.forename: A
authors.initials: A
authors.lastname: Burgos
authors.forename: M
authors.initials: M
authors.lastname: Tellaetxe
authors.forename: R
authors.initials: R
authors.lastname: Cisterna
authors.forename: J
authors.initials: J
authors.lastname: Pontón
mesh_terms: D000362:Agar; D002175:Candida / Q000145:classification* / Q000254:growth & development; D016533:Mycological Typing Techniques; D015203:Reproducibility of Results; D013778:Tetrazolium Salts
chemical_list: D013778:Tetrazolium Salts; C009591:triphenyltetrazolium; D000362:Agar