title: Bacteriostatic effect of 4,7-dicyanobenzofurazan due to inactivation of 2,3-dihydroxyisovalerate dehydratase.
abstract: As part of our research on benzofurazans (BZs), we have reported the bacterioses of BZs in Escherichia coli, which may be due to O2-. produced within E. coli in the presence of dioxygen (O2). Incubation of E. coli with 4,7-dicyanobenzofurazan (1) lowered the 2,3-dihydroxyisovalerate dehydratase activity detectable in extracts from these cells. Addition of branched chain amino acids such as valine and leucine protected E. coli from growth inhibition by compound 1, though it could not protect E. coli from the damage by paraquat (PQ). Addition of Fe(III)-tris[N-(2-pyridylmethyl)-2-aminoethyl]amine (Fe-TPAA), a novel superoxide dismutase mimic, protected the dehydratase in a dose-dependent manner, which confirms that inactivation of the dehydratase is largely due to production of O2-.. The possibility was discussed that the bacteriostatic effect of compound 1 is due to the inactivation of 2,3-dihydroxyisovalerate dehydratase.
authors.affiliation: College of Pharmacy, Nihon University, Chiba, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Takabatake
authors.forename: M
authors.initials: M
authors.lastname: Hasegawa
authors.forename: T
authors.initials: T
authors.lastname: Nagano
authors.forename: M
authors.initials: M
authors.lastname: Hirobe
mesh_terms: D001419:Bacteria / Q000187:drug effects*; D001583:Benzoxazoles / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects; D006131:Growth Inhibitors / Q000494:pharmacology; D006836:Hydro-Lyases / Q000037:antagonists & inhibitors*; D010100:Oxygen / Q000633:toxicity*
chemical_list: D001583:Benzoxazoles; D006131:Growth Inhibitors; C073146:4,7-dicyanobenzofurazan; D006836:Hydro-Lyases; C020052:dihydroxyacid dehydratase; D010100:Oxygen