Automated quantitative nephelometric latex immunoassay for determining ferritin in human serum.
We describe a rapid and sensitive latex nephelometric immunoassay for quantifying ferritin in human serum. This latex immunoassay procedure uses commercially available ready-for-use reagents [Tina-Quant (a) Ferritin, Boehringer Mannheim] that have a long shelf life. The assay consists of incubating the diluted serum sample (5-fold) for 12 min at room temperature with latex particles covalently coated with anti-ferritin antibodies, and then quantifying the change of light-scatter produced. The assay is fully automated on the Behring nephelometer analyzer with a sampling rate of 150 samples/hour. The method has an analytical range of 3 to 260 micrograms/l. Maximal intra- and inter-assay CVs were 4.0 and 6.2%, respectively. Analytical recoveries ranged from 91.3 to 103.6%. Assay detection limit was less than 3 micrograms/l. Linearity of the test is given throughout the measuring range. There was no interference from bilirubin (up to 340 mumol/l), haemoglobin (up to 7 g/l), or rheumatoid factor (up to 1,100 IU/ml). Turbid and lipemic samples interfere. This interference may be avoided by pretreating these samples prior to assay. Results correlated well with those obtained by an automated ELISA test (r = 0.995) and with those of two commercial RIA methods (r greater than 0.97). This latex nephelometric procedure is a convenient method and represents an interesting alternative to other immunoassays for measuring ferritin in human serum.
Borque
L
L
Laboratorio Central, Hospital San Millan, Logroño, Spain.
Rus
A
A
del Cura
J
J
Maside
C
C
Escanero
J
J
D000293:Adolescent; D000328:Adult; D005069:Evaluation Studies as Topic; D005260:Female; D005293:Ferritins / Q000097:blood*; D006801:Humans; D007841:Latex Fixation Tests / Q000379:methods* / Q000706:statistics & numerical data; D008297:Male; D008875:Middle Aged; D009391:Nephelometry and Turbidimetry; D012016:Reference Values; D012680:Sensitivity and Specificity
D005293:Ferritins