Polyacrylamide gel electrophoresis in vertical, inverse and double-crossing gradients of soluble polymers.
The presence of soluble dextrans, methylcellulose and polyethylene glycol polymers incorporated into vertical sodium dodecyl sulfate (SDS)-polyacrylamide slabs during electrophoresis can have a pronounced effect on protein separations. The effects of various standard and inverse gradients of polymers on the electrophoretic mobility of marker proteins in 10% T, 2.66% C Laemmli-style SDS gels, and the effects of simultaneous pore size and polymer gradients were investigated. These experiments demonstrate that the inclusion of polymers is a new, additional parameter that can be useful in resolving complex mixtures of proteins.
Gersten
D M
DM
Department of Pathology, Georgetown University Medical Center, Washington, DC 20007.
Bijwaard
K E
KE
D003911:Dextrans / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D005782:Gels; D008747:Methylcellulose; D011108:Polymers; D011506:Proteins / Q000737:chemistry*; D012995:Solubility
D003911:Dextrans; D005782:Gels; D011108:Polymers; D011506:Proteins; D008747:Methylcellulose