Molecular evidence for the presence of chlamydia in the synovium of patients with Reiter's syndrome.
OBJECTIVE
There is much evidence indicating that chlamydial antigens in the synovium may be critical in the pathogenesis of Reiter's syndrome (RS), but it is not known whether intact organisms are present in that tissue in any stage of the disease. The present study was undertaken to begin to address this question.


METHODS
We used a highly specific and sensitive molecular hybridization screening system which detects chlamydial RNA, to examine synovial biopsy samples from 22 patients with various arthropathies, including 9 with RS.


RESULTS
Seven of the 9 RS patients were positive for chlamydial RNA, while 3 of the 13 non-RS patients were also positive; positive results in the non-RS patients probably indicate the actual presence of the organism, since these patients had arthritis that was otherwise incompletely explained.


CONCLUSIONS
The detection of chlamydial RNA, in combination with previous findings of chlamydia-like particles and/or chlamydial antigens in the synovium of RS patients, suggests that whole bacterial cells are present in that tissue.
Rahman
M U
MU
Department of Microbiology and Immunology, Medical College of Pennsylvania, Philadelphia.
Cheema
M A
MA
Schumacher
H R
HR
Hudson
A P
AP
D000293:Adolescent; D000328:Adult; D016918:Arthritis, Reactive / Q000382:microbiology*; D002689:Chlamydia / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009693:Nucleic Acid Hybridization; D012329:RNA, Bacterial / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D013582:Synovial Fluid / Q000382:microbiology*; D013583:Synovial Membrane / Q000382:microbiology*
D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S