title: Subtyping of human immunodeficiency virus isolates with a panel of monoclonal antibodies: identification of conserved and divergent epitopes on p17 and p25 core proteins.
abstract: We have investigated the feasibility and significance of subtyping of human immunodeficiency virus (HIV) isolates with monoclonal antibodies (mAb) raised against the core proteins of HIV. A panel of 37 mAb tested for reactivity with HIV1 oligopeptides was used to analyse the antigenic relatedness among 14 HIV isolates which included 12 isolates of HIV1 from different geographical origins and 2 isolates of HIV2. Three out of these 37 mAb reacted with conserved epitopes expressed by all 14 HIV isolates tested. These reagents which included 2 mAb reacting with the 285-310 amino acid sequence of p25 and 1 mAb reacting with an epitope of p25 not mapped by the peptides' approach, also reacted with a non-human primate lentivirus. Five mAb reacting either with the 11-25 or 121-132 amino acid sequences of p17 or the 302-320 amino acid sequence of p25 reacted with strain-specific epitopes. The other 29 mAb reacted with polymorphic epitopes and thereby define subfamily and subtype-specific markers.
authors.affiliation: CRBM du CNRS, Centre de Tri des Molécules Anti-HIV, Institut de Biologie-Faculté de Médecine, Montpellier, France.
authors.forename: V
authors.initials: V
authors.lastname: Robert-Hebmann
authors.forename: S
authors.initials: S
authors.lastname: Emiliani
authors.forename: M
authors.initials: M
authors.lastname: Resnicoff
authors.forename: F
authors.initials: F
authors.lastname: Jean
authors.forename: C
authors.initials: C
authors.lastname: Devaux
mesh_terms: D000911:Antibodies, Monoclonal; D001483:Base Sequence; D000090266:Chromobox Protein Homolog 5; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000235:genetics*; D015683:Gene Products, gag / Q000235:genetics*; D005814:Genes, Viral / Q000235:genetics*; D015488:HIV Antigens / Q000235:genetics*; D015497:HIV-1 / Q000276:immunology*; D015498:HIV-2 / Q000276:immunology*; D016130:Immunophenotyping; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D012689:Sequence Homology, Nucleic Acid; D015302:Simian Immunodeficiency Virus / Q000276:immunology; D014764:Viral Proteins; D054301:gag Gene Products, Human Immunodeficiency Virus
chemical_list: D000911:Antibodies, Monoclonal; C000715594:CBX5 protein, human; D000939:Epitopes; D015683:Gene Products, gag; D015488:HIV Antigens; C059827:HIV gag protein p25; D014764:Viral Proteins; D054301:gag Gene Products, Human Immunodeficiency Virus; C054858:p17 protein, Human Immunodeficiency Virus Type 1; D000090266:Chromobox Protein Homolog 5