Investigation of the applicability of a tensile testing machine for measuring mucoadhesive strength.
The applicability of a tensile testing machine (M30K, JJ Lloyd Instruments Ltd, GB) is investigated for measuring mucoadhesive strengths. A sample of an aqueous dispersion of a polymer with expected mucoadhesive properties is placed between two homemade discs of polyoxymethylene. The upper disc is mounted on a movable part of the machine while the lower disc is fixed on the stationary frame. A tensile force is submitted and the maximum detachment force at fracture and the adhesion work are estimated from the force displacement curve recorded. In some experiments, native mucous tissue of the large intestine of pigs was glued to the upper disc. Four polymers polycarbophil (Carbopol EX-55), carboxypolymethylene (Carbopol 934P), hydroxypropylmethylcellulose (Methocel K4M), and sodium alginate, are used in five different concentrations. At least three measurements are made of each polymer and concentration. Viscosity and osmolality are determined. By standardizing the time of sample equilibration and the run rate before measurement, it is possible to get good reproducibility of the tensile values. Based on the maximum nominal breaking force and the work consumed, it is concluded that the tensile strength is dependent both on the concentration and the type of polymer. The conclusions are the same independent of whether mucous pig tissue is used, or not. The same rank order in adhesive properties of the polymers is achieved as from using modified surface tensiometers.
Dyvik
K
K
Institute of Pharmacy, University of Oslo, Norway.
Graffner
C
C
D000268:Adhesiveness; D000818:Animals; D066298:In Vitro Techniques; D007420:Intestine, Large / Q000502:physiology; D008722:Methods; D011786:Quality Control; D013552:Swine; D013678:Technology, Pharmaceutical / Q000295:instrumentation; D013718:Tensile Strength; D014783:Viscosity