Endocytosis of thyroglobulin is not mediated by mannose-6-phosphate receptors in thyrocytes. Evidence for low-affinity-binding sites operating in the uptake of thyroglobulin.
Thyroglobulin, the major secretory product of thyrocytes, is the macromolecular precursor of thyroid hormones. After its synthesis, thyroglobulin follows a complex secretion, storage and recapture pathway to lysosomes. Porcine thyroglobulin was shown to carry the mannose 6-phosphate-(Man6P)-recognition marker on its N-linked glycans. Since the cation-independent Man6P receptor could also be found on the apical plasma membrane of porcine thyrocytes, we examined the significance of the Man6P signal for the transport of thyroglobulin. Here, we present data implying that Man6P receptors are not relevant for endocytosis of thyroglobulin in thyrocytes. Instead, we provide evidence for the existence of specific, low-affinity-binding sites for thyroglobulin on the apical plasma membrane of thyrocytes responsible for endocytosis of thyroglobulin. Binding studies with intact, polar-organized porcine thyrocytes grown on collagen-coated filters revealed cooperative and saturable binding of thyroglobulin to the apical-plasma-membrane domain at relatively high concentrations of thyroglobulin (20 microM). These observations show that low-affinity interactions between thyroglobulin and the apical plasma membrane play a key role in endocytosis of thyroglobulin and hormone formation in the thyroid. The data in this publication have been published as an abstract [Lemansky, P. and Herzog, V. (1991) J. Cell Biol. 115, 261a].
Lemansky
P
P
Institute of Cell Biology, Rheinische Friedrich-Wilhelms-Universität Bonn, FRG.
Herzog
V
V
D000042:Absorption; D000818:Animals; D001692:Biological Transport; D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured; D002846:Chromatography, Affinity; D004705:Endocytosis; D007457:Iodine Radioisotopes; D008360:Mannosephosphates / Q000494:pharmacology; D008715:Methionine / Q000378:metabolism; D008970:Molecular Weight; D010873:Pinocytosis; D017527:Receptor, IGF Type 2 / Q000502:physiology*; D013462:Sulfur Radioisotopes; D013552:Swine; D013954:Thyroglobulin / Q000302:isolation & purification / Q000378:metabolism*; D013961:Thyroid Gland / Q000378:metabolism*
D007457:Iodine Radioisotopes; D008360:Mannosephosphates; D017527:Receptor, IGF Type 2; D013462:Sulfur Radioisotopes; C027693:mannose-6-phosphate; D013954:Thyroglobulin; D008715:Methionine