Oxygen-transport and solution properties of polylipid/Hb vesicles (ARC).
Polymerized phospholipid vesicle encapsulating Hb (polylipid/Hb vesicle) was prepared from a mixture of unsaturated phospholipid, cholesterol and unsaturated fatty acid and polymerization by gamma-ray irradiation. The average radius of resulting vesicles was 203 +/- 39 nm and concentrated Hb (30 wt%) was efficiently encapsulated. gamma-Ray polymerization proceeds theoretically at low temperature (4 degrees C). P50 and oxygen transporting efficiency were adjusted to 40 mmHg and 40%, respectively. Oncotic pressure and solution viscosity can be controlled to the same values as blood.
Takeoka
S
S
Department of Polymer Chemistry, Waseda University, Tokyo, Japan.
Hasegawa
E
E
Nishide
H
H
Tsuchida
E
E
Sekiguchi
S
S
D001802:Blood Substitutes / Q000737:chemistry* / Q000302:isolation & purification; D006454:Hemoglobins / Q000737:chemistry* / Q000302:isolation & purification; D006801:Humans; D008081:Liposomes; D009997:Osmotic Pressure; D010100:Oxygen; D012996:Solutions; D014783:Viscosity
D001802:Blood Substitutes; D006454:Hemoglobins; D008081:Liposomes; D012996:Solutions; C037158:polyhemoglobin; D010100:Oxygen