Analysis of tick-borne encephalitis virus antigens by monoclonal antibodies.
Monoclonal antibodies (MAbs) against Central European tick-borne encephalitic virus, strain Hypr, were used for determination and characterization of viral antigens of infected PS cells. The MAbs reacted in immunoblotting with flavivirus glycoprotein E (56 kD), and nonstructural protein NS3 (70 kD). According to enzyme immunoassay with infected cells, NS3 antigen is expressed in the plasmalemma.
Grubhoffer
L
L
Institute of Parasitology, Czechoslovak Academy of Sciences, Budĕjovice.
Kopecký
J
J
Tomková
E
E
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D000956:Antigens, Viral / Q000032:analysis*; D002478:Cells, Cultured; D004591:Electrophoresis, Polyacrylamide Gel; D004669:Encephalitis Viruses, Tick-Borne / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000032:analysis; D006825:Hybridomas; D015151:Immunoblotting
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D000939:Epitopes