title: Action of metoprolol, enalapril, diltiazem, verapamil, and nifedipine on cell growth of vascular smooth muscle cells.
abstract: The influence of nifedipine, verapamil, diltiazem, metoprolol, and enalapril on the basal and angiotensin II (Ang II)-induced elevation of [3H]thymidine incorporation into vascular smooth muscle cell (VSMC) DNA was examined. Our results from four independent experiments, each performed in triplicate, are summarized by calculating the half-maximal inhibitory concentration (IC50) of the drugs. Nifedipine, verapamil, and diltiazem had IC50 values of 2.3 +/- 0.7 x 10(-6), 3.5 +/- 0.3 x 10(-6), and 6.6 +/- 2.8 x 10(-6) M, respectively. Metoprolol had an IC50 value of 49 +/- 16 x 10(-6) M, whereas enalapril was completely ineffective. All drugs used had no influence on the basal cell [3H]thymidine incorporation. This in vitro study allows one to conclude that the calcium-entry blockers can inhibit the Ang II-induced cell growth and thus may have beneficial effects on the development and regression of vascular growth, which is associated with the pathogenesis of cardiovascular diseases.
authors.affiliation: Medizinische Universitäts-Poliklinik Bonn, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Sachinidis
authors.forename: Y
authors.initials: Y
authors.lastname: Ko
authors.forename: G K
authors.initials: GK
authors.lastname: Graack
authors.forename: A J
authors.initials: AJ
authors.lastname: Wieczorek
authors.forename: H
authors.initials: H
authors.lastname: Vetter
mesh_terms: D000818:Animals; D002455:Cell Division / Q000187:drug effects; D004110:Diltiazem / Q000494:pharmacology*; D004656:Enalapril / Q000494:pharmacology*; D008790:Metoprolol / Q000494:pharmacology*; D009131:Muscle, Smooth, Vascular / Q000187:drug effects* / Q000378:metabolism; D009543:Nifedipine / Q000494:pharmacology*; D051381:Rats; D011921:Rats, Inbred WKY; D013936:Thymidine / Q000378:metabolism
chemical_list: D004656:Enalapril; D004110:Diltiazem; D008790:Metoprolol; D009543:Nifedipine; D013936:Thymidine