[Possibility of selective binding of HIV-1 infected cells by immunosorbents].
A study was made of a possibility of specific sorption of HIV-1 infected cells with gamma-fraction of AIDS patients' serum containing high titer-specific antibodies, immobilized on the silica matrix (C-3). The laboratory tests were made with the use of the monocyte culture [symbol: see text] chronically infected with HIV-1. In addition to a decrease of the cell count after sorption, there was a decline of the activity of antigen material in the samples (to 43% of the initial). The selectivity rates were 0.92 for immunosorbents and 0.29 for sorbents of the control group, that confirms and increase of the delay of the cells expressing HIV-1 proteins on a column with the immobilized gamma-fraction of AIDS patients' serum. The use of the synthesized immunosorbents in vivo may appear debatable. However, this method can be employed for preparative purposes.
Lopukhin
Iu M
IuM
Pavlenko
V V
VV
Kulaev
D V
DV
Alekseĭchuk
V S
VS
Karamov
E V
EV
Rudneva
I A
IA
Petrunin
D D
DD
Liukova
T K
TK
D000163:Acquired Immunodeficiency Syndrome / Q000276:immunology / Q000378:metabolism; D002452:Cell Count; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D015483:HIV Antibodies / Q000032:analysis; D015497:HIV-1 / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D007164:Immunosorbents; D014764:Viral Proteins / Q000378:metabolism
D015483:HIV Antibodies; D007164:Immunosorbents; D014764:Viral Proteins