Human and murine monoclonal antibodies directed against a conserved sequence from gp41 (aa583-599) of human immunodeficiency virus type 1.
Human spleen cells from an HIV-seropositive donor were immunized in vitro with the aa583-599 peptide conjugated to an heptalysyl core. This sequence was derived from the putatively HIV-immunosuppressive region of HIV1 gp41. The same conjugated peptide was used to immunize mice. One human and one mouse IgM monoclonal antibody (mAb) directed against the aa583-599 peptide were obtained. The two mAb had distinct patterns of reactivity against a panel of 42 peptides with modified sequences. Neither of the mAb inhibited the immunosuppressive effect of aa583-599 octopus-lys-conjugated peptide on anti-CD3 Ab-induced lymphoproliferation. In addition, both mAb did not neutralize cell-free virus transmission or enhance HIV infection. However, HmAb inhibited formation of syncytia between HIV1-infected (but not HIV2-infected cells) and non-infected target cells at concentrations above 20 micrograms/ml, whereas MmAb did not have any effect. The degree of conservation of the aa583-599 region makes HmAb a candidate for use as a group-specific reagent in future HIV1 passive immunotherapy protocols.
Ebersold
A
A
Unité de Recherche sur les Hépatites, INSERM U271, Lyon, France.
Boyer
V
V
Klasse
P J
PJ
Holnigue
M
M
Fraisier
C
C
Cocchi
J M
JM
Pipkorn
R
R
Blomberg
J
J
Desgranges
C
C
D000293:Adolescent; D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000737:chemistry*; D001666:Binding Sites, Antibody; D002474:Cell-Free System / Q000276:immunology; D000939:Epitopes / Q000737:chemistry / Q000276:immunology; D015700:HIV Envelope Protein gp41 / Q000276:immunology*; D006801:Humans; D007108:Immune Tolerance; D051379:Mice; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D010446:Peptide Fragments / Q000276:immunology*; D013045:Species Specificity; D013154:Spleen / Q000276:immunology
D000911:Antibodies, Monoclonal; D000939:Epitopes; D015700:HIV Envelope Protein gp41; D010446:Peptide Fragments