title: Pitfalls in the double labelling immunoelectron microscopy using one face of the grid.
abstract: We compared two procedures in the double labelling immunoelectron microscopic method of hormone-storing cells: one-faced and two-faced procedures. With the two-faced procedure we got convincing results consistent with morphological finding, while we often got false results with the one-faced procedure. By the misleading one-faced procedure, the cell storing any one hormone will be misinterpreted as a multihormone storing cell. Therefore, it is concluded that the two-faced method is safer for the double labelling immunoelectron microscopic study.
authors.affiliation: Department of Pathology, Kitasato University School of Medicine, Kanagawa, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Furuhata
authors.forename: N
authors.initials: N
authors.lastname: Nemoto
authors.forename: S
authors.initials: S
authors.lastname: Miyazawa
authors.forename: M
authors.initials: M
authors.lastname: Otani
authors.forename: S
authors.initials: S
authors.lastname: Toya
authors.forename: T
authors.initials: T
authors.lastname: Kameya
mesh_terms: D000681:Amylases / Q000032:analysis / Q000276:immunology; D013006:Growth Hormone / Q000032:analysis / Q000276:immunology; D006801:Humans; D007106:Immune Sera / Q000032:analysis / Q000276:immunology; D007150:Immunohistochemistry; D016253:Microscopy, Immunoelectron / Q000295:instrumentation* / Q000379:methods*; D010903:Pituitary Gland, Anterior / Q000737:chemistry / Q000166:cytology / Q000648:ultrastructure; D011388:Prolactin / Q000032:analysis / Q000276:immunology
chemical_list: D007106:Immune Sera; D011388:Prolactin; D013006:Growth Hormone; D000681:Amylases