Intercellular transport through a partially denuded arterial endothelial monolayer. Effect of platelets and PGI2.
Fluorescein Dextran (FD) was shown to be transported at increased rates through partially denuded endothelial monolayer. Platelet binding to the partially denuded monolayer lowered transport rates to those comparable with intact endothelium. Inhibition of transport by platelet binding was not affected by the addition of isocarbacyclin (a stable derivative of PGI2). This result suggests that adherent platelets at the partial denudation site are sufficient to suppress transport of FD.
Yagyu
Y
Y
Department of Microbiology and Molecular Pathology, Faculty of Pharmaceutical Sciences, Teikyo University, Kanagawa, Japan.
Mineo
C
C
Imanaka
T
T
Ikegami
S
S
Takano
T
T
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D001792:Blood Platelets / Q000502:physiology*; D002478:Cells, Cultured; D003864:Depression, Chemical; D003911:Dextrans / Q000493:pharmacokinetics*; D004730:Endothelium, Vascular / Q000378:metabolism*; D011464:Epoprostenol / Q000031:analogs & derivatives* / Q000494:pharmacology; D016650:Fluorescein-5-isothiocyanate / Q000493:pharmacokinetics* / Q000031:analogs & derivatives; D010973:Platelet Adhesiveness / Q000187:drug effects; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology*; D011465:Prostaglandins, Synthetic / Q000494:pharmacology; D011817:Rabbits; D013552:Swine
D003911:Dextrans; D010975:Platelet Aggregation Inhibitors; D011465:Prostaglandins, Synthetic; C015219:fluorescein isothiocyanate dextran; C041946:9-O-methanoprostaglandin I; D011464:Epoprostenol; D016650:Fluorescein-5-isothiocyanate