Identification and characterization of the cis-elements regulating the rat AMOG (adhesion molecule on glia)/Na,K-ATPase beta 2 subunit gene.
We have identified a positive regulatory cis-acting element of the adhesion molecule on glia (AMOG)/Na,K-ATPase beta 2 subunit gene as GAGGCGGGG at position -87 to -79 by transient transfection assay using B103 cells (rat neuroblastoma cell line). The newly identified AMOG regulatory element (AMRE) enhanced the promoter activity in a mutually compensating manner with the Sp1 element at position -147 to -142. AMRE acts as a positive regulatory element not only in B103 cells but also in 3Y1 (rat embryo cell line) cells to roughly the same extent and in MDCK (canine kidney cell line) cells to a lesser extent. AMRE also enhances other gene promoters, such as myelin basic protein (MBP) and herpes simplex virus (HSV) thymidine kinase (TK) gene promoters. The element is not a typical enhancer element because when it is introduced downstream of the HSV TK promoter, it has no enhancing activity.
Kawakami
K
K
Department of Biology, Jichi Medical School, Tochigi.
Suzuki-Yagawa
Y
Y
Watanabe
Y
Y
Nagano
K
K
D000251:Adenosine Triphosphatases; D000818:Animals; D001483:Base Sequence; D027682:Cation Transport Proteins; D015816:Cell Adhesion Molecules, Neuronal / Q000235:genetics* / Q000502:physiology; D002478:Cells, Cultured; D002874:Chromosome Mapping; D004285:Dogs; D016326:Extracellular Matrix Proteins / Q000235:genetics* / Q000502:physiology; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology; D005809:Genes, Regulator / Q000502:physiology*; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D009154:Mutation; D009447:Neuroblastoma / Q000201:enzymology / Q000235:genetics; D012313:RNA / Q000235:genetics; D051381:Rats; D012260:Ribonucleases / Q000378:metabolism; D000254:Sodium-Potassium-Exchanging ATPase / Q000235:genetics* / Q000502:physiology; D014158:Transcription, Genetic / Q000235:genetics; D014162:Transfection
C504192:Atp1b2 protein, rat; D027682:Cation Transport Proteins; D015816:Cell Adhesion Molecules, Neuronal; D016326:Extracellular Matrix Proteins; D046911:Macromolecular Substances; D012313:RNA; D012260:Ribonucleases; D000251:Adenosine Triphosphatases; D000254:Sodium-Potassium-Exchanging ATPase