Acute reflux pancreatitis in rats: a comparison between two experimental models.
This study was undertaken in order to compare the reliability of two acute reflux pancreatitis models in rats, one performed by positioning a silicon tube in the duodenum and the other by creating a gastro-jejunal anastomosis. In two groups (A = 10 and B = 10 rats) a silicon tube was positioned in the duodenum; in the remaining two groups (C = 12 and D = 6 rats) a latero-lateral antecolic anisoperistaltic gastro-jejunal anastomosis was performed 30 days before surgery. A closed duodenal loop was created for 12 hours in groups A and C but not in B and D. Rats in both groups A and C developed acute pancreatic inflammation of a mild degree. Sham operated rats with silicon tube placement had higher histological damage scores than those with gastro-jejunal anastomosis. The difference found between the two groups of rats which underwent gastro-jejunal anastomosis was more marked than that between the two groups which had silicon tube placement. It was concluded that the creation of a gastro-jejunal anastomosis is probably the safer procedure to allow gastro-intestinal flow in acute reflux pancreatitis in rats.
Infantino
A
A
Istituto di Patologia Chirurgica I, Universita di Padova, Italy.
Fabris
C
C
Basso
D
D
Del Favero
G
G
Munaretto
S
S
Plebani
M
M
Panozzo
M P
MP
Meggiato
T
T
Fassina
A
A
Lise
M
M
D000681:Amylases / Q000097:blood; D000714:Anastomosis, Surgical; D000818:Animals; D004195:Disease Models, Animal; D004386:Duodenum; D004487:Edema / Q000473:pathology; D005218:Fat Necrosis / Q000473:pathology; D006470:Hemorrhage / Q000473:pathology; D007419:Intestinal Secretions; D007440:Intubation / Q000295:instrumentation; D007583:Jejunum / Q000601:surgery; D008297:Male; D009336:Necrosis; D010195:Pancreatitis / Q000201:enzymology / Q000209:etiology* / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012828:Silicones; D013270:Stomach / Q000601:surgery
D012828:Silicones; D000681:Amylases