title: Substance P immunoreactivity of rat brain stem neurons in primary culture.
abstract: Substance P (SP)-ergic neurons from 16/17 day-embryonic rat brain stem in primary culture were identified by immunocytochemistry using biotinylated avidin and phosphatase alkaline methods with affinity-purified anti-SP antibodies. An average of 84% of neurons contained SP from day 9 to day 21 after plating. These in vitro data show that SP-containing neurons develop in our culture conditions. SP may act as a maturation factor as well as a neurotransmitter.
authors.affiliation: Laboratoire de Biologie Fonctionnelle du Neurone, CNRS, Gif sur Yvette Cx, France.
authors.forename: T D
authors.initials: TD
authors.lastname: Jacquin
authors.forename: J P
authors.initials: JP
authors.lastname: Denizot
authors.forename: M
authors.initials: M
authors.lastname: Denavit-Saubie
mesh_terms: D000818:Animals; D001933:Brain Stem / Q000737:chemistry / Q000166:cytology* / Q000196:embryology; D002452:Cell Count; D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured; D009474:Neurons / Q000737:chemistry*; D051381:Rats; D011919:Rats, Inbred Strains / Q000196:embryology; D013373:Substance P / Q000032:analysis* / Q000502:physiology
chemical_list: D013373:Substance P