Identification of a protein that binds to the SH3 region of Abl and is similar to Bcr and GAP-rho.
A Src homology 3 (SH3) region is a sequence of approximately 50 amino acids found in many nonreceptor tyrosine kinases and other proteins. Deletion of the SH3 region from the protein encoded by the c-abl proto-oncogene activates the protein's transforming capacity, thereby suggesting the participation of the SH3 region in the negative regulation of transformation. A complementary DNA was isolated that encoded a protein, 3BP-1, to which the SH3 region of Abl bound with high specificity and to which SH3 regions from other proteins bound differentially. The sequence of the 3BP-1 protein is similar to that of a COOH-terminal segment of Bcr and to guanosine triphosphatase-activating protein (GAP)-rho, which suggests that it might have GAP activity for Ras-related proteins. The 3BP-1 protein may therefore be a mediator of SH3 function in transformation inhibition and may link tyrosine kinases to Ras-related proteins.
Cicchetti
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P
Rockefeller University, New York, NY 10021.
Mayer
B J
BJ
Thiel
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G
Baltimore
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D
D016475:3T3 Cells; D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D002678:Chimera; D003001:Cloning, Molecular; D020690:GTPase-Activating Proteins; D015723:Gene Library; D016313:Genes, abl; D016391:Genes, src; D005982:Glutathione Transferase / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D015513:Oncogene Proteins / Q000235:genetics / Q000378:metabolism*; D010957:Plasmids; D016133:Polymerase Chain Reaction / Q000379:methods; D016548:Prosencephalon / Q000502:physiology; D011505:Protein-Tyrosine Kinases / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism*; D011518:Proto-Oncogene Proteins; D016315:Proto-Oncogene Proteins c-abl / Q000235:genetics / Q000378:metabolism*; D051562:Proto-Oncogene Proteins c-bcr; D016392:Proto-Oncogene Proteins pp60(c-src) / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D015183:Restriction Mapping; D012234:Rho Factor / Q000378:metabolism*; D012689:Sequence Homology, Nucleic Acid; D020703:ras GTPase-Activating Proteins
D020690:GTPase-Activating Proteins; D015513:Oncogene Proteins; D011506:Proteins; D011518:Proto-Oncogene Proteins; D011993:Recombinant Fusion Proteins; D012234:Rho Factor; D020703:ras GTPase-Activating Proteins; D005982:Glutathione Transferase; D011505:Protein-Tyrosine Kinases; D016315:Proto-Oncogene Proteins c-abl; D016392:Proto-Oncogene Proteins pp60(c-src); C496614:Bcr protein, mouse; D051562:Proto-Oncogene Proteins c-bcr