Bio-antimutagenic activities of vitamin B6 in E. coli and mouse peripheral blood cells.
Pyridoxal (PL) and pyridoxal 5'-phosphate (PLP) showed a marked bio-antimutagenic effect on UV-induced mutagenesis in E. coli B/r WP2, but not in the DNA excision repair-deficient strain WP2suvrA under the condition where no cellular toxicity was observed. No delay in the first cell division was seen on post-treatment with PL after UV irradiation. PL reduced not only UV- but 4-nitroquinoline-1-oxide-induced mutation, while it was ineffective in N-methyl-N'-nitro-N-nitrosoguanidine- or gamma-ray-treated cells. These results suggest that PL promotes DNA excision repair directly or indirectly and the decrease in the amount of unrepaired DNA damage might cause the reduction of UV-induced mutations in E. coli B/r WP2. In addition to the above observation, PLP reduced the frequency of mitomycin C- (2 mg/kg, i.p.) induced micronuclei in mouse peripheral blood cells. Simultaneous or subsequent oral administration of PLP (25 mg/kg) decreased the frequency of micronucleated peripheral reticulocytes.
Shimoi
K
K
Laboratory of Health Science, School of Pharmaceutical Sciences, University of Shizuoka, Japan.
Akaiwa
E
E
Mori
N
N
Sano
M
M
Nakamura
Y
Y
Tomita
I
I
D000818:Animals; D016587:Antimutagenic Agents / Q000494:pharmacology*; D001773:Blood Cells / Q000187:drug effects; D004260:DNA Repair / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D011730:Pyridoxal / Q000494:pharmacology; D011732:Pyridoxal Phosphate / Q000494:pharmacology; D011736:Pyridoxine / Q000494:pharmacology*
D016587:Antimutagenic Agents; D011730:Pyridoxal; D011732:Pyridoxal Phosphate; D011736:Pyridoxine