Characterization and immune response of a protein produced by a cDNA clone of foot and mouth disease virus, type Asia 1 63/72.
A 0.9 kb double stranded cDNA of foot and mouth disease virus (FMDV) Type Asia 1, 63/72 was cloned in an expression vector, pUR222. A protein of 38 kd was produced by the clone which reacted with the antibodies raised against the virus. A 20 kd protein which may be derived from the 38 kd protein contained the antigenic epitopes of the protein VP1 of the virus. Injection of 10-20 micrograms of the partially purified 38 and 20 kd proteins or a lysate of cells containing 240 micrograms of the proteins elicited high titers of FMDV specific antibodies in guinea pigs and cattle respectively. Also, at these concentrations, the proteins protected 5 of 8 guinea pigs and 3 of 8 cattle when challenged with a virulent virus.
Suryanarayana
V V
VV
Indian Veterinary Research Institute, Bangalore.
Venkataramanan
R
R
Rao
B U
BU
Padayatty
J D
JD
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D005537:Aphthovirus / Q000235:genetics* / Q000276:immunology; D002213:Capsid / Q000235:genetics* / Q000276:immunology; D036022:Capsid Proteins; D002417:Cattle; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000235:genetics*; D000939:Epitopes / Q000235:genetics; D005536:Foot-and-Mouth Disease / Q000517:prevention & control; D006168:Guinea Pigs; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D014614:Vaccines, Synthetic / Q000302:isolation & purification; D014765:Viral Vaccines / Q000302:isolation & purification
D000914:Antibodies, Viral; D036022:Capsid Proteins; D004279:DNA, Viral; D000939:Epitopes; D011994:Recombinant Proteins; C442501:VP1 protein, Foot-and-mouth disease virus; D014614:Vaccines, Synthetic; D014765:Viral Vaccines