title: Expression of mRNAs for alpha-fetoprotein (AFP) and albumin and incorporation of AFP and docosahexaenoic acid in baboon fetuses.
abstract: alpha-Fetoprotein and albumin, two members of a multigene family, reversibly bind fatty acids with high affinity. The origin of alpha-fetoprotein (AFP) and albumin present in fetal tissues other than the liver and yolk sac is a subject of controversy. In this work, we have searched for the presence of the albumin and AFP mRNA molecules in different fetal organs of the baboon (Papio cinocephalus), using a highly sensitive gel-blot hybridization assay with human albumin and AFP cDNA probes. Large amounts of albumin and AFP mRNA molecules were found in the fetal liver; significant quantities were also present in the gastrointestinal tract and in the kidney. No detectable levels were found in the other tissues examined (brain, skin, spleen, pancreas, muscle, heart, thymus, placenta, and amnion). After injection of radiolabeled AFP into pregnant baboons, all fetal tissues took up the protein. White adipose tissue, kidney, intestine, lung, liver, and cerebral cortex showed a great uptake of exogenous AFP. [14C]Docosahexaenoic acid (22:6, n-3), injected at the same time, was actively transferred from the maternal compartment across the placenta and incorporated into cellular lipids by all fetal tissues and particularly by liver (around 70% of total incorporation). The levels of [14C]docosahexaenoic acid per gram of tissue increased in the order: maternal blood less than placenta less than fetal liver, indicating a selective accumulation of this fatty acid by the fetus. These results indicate that intracellular AFP in non-hepatic tissues of the developing baboon is, for the most part, of plasma origin.
authors.affiliation: Institut de Recherches Scientifiques sur le Cancer, B.P. 8, Villejuif, France.
authors.forename: J
authors.initials: J
authors.lastname: Naval
authors.forename: M
authors.initials: M
authors.lastname: Calvo
authors.forename: J
authors.initials: J
authors.lastname: Laborda
authors.forename: P
authors.initials: P
authors.lastname: Dubouch
authors.forename: M
authors.initials: M
authors.lastname: Frain
authors.forename: J M
authors.initials: JM
authors.lastname: Sala-Trepat
authors.forename: J
authors.initials: J
authors.lastname: Uriel
mesh_terms: D000418:Albumins / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D015342:DNA Probes; D004281:Docosahexaenoic Acids / Q000378:metabolism; D005260:Female; D005333:Fetus / Q000378:metabolism*; D008099:Liver / Q000378:metabolism; D010215:Papio; D011247:Pregnancy; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D014018:Tissue Distribution; D000509:alpha-Fetoproteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D000418:Albumins; D015342:DNA Probes; D012333:RNA, Messenger; D000509:alpha-Fetoproteins; D004281:Docosahexaenoic Acids