[Effects of mepartricin on the growth of hypertrophic prostatic tissue in primary culture. Creation of the experimental model and preliminary results].
The aim of this study was to standardise a method for the in vitro culture of hypertrophic prostatic tissue, to assay the morphological type of isolated cells, to evaluate the degree of proliferation and to identify their differentiated iter. In addition, the effects of mepartricina on growth was also studied obtaining preliminary results. Of a total of 40 biopsies used in this experiment, 30 were placed directly in culture using Freshney's method, whereas the remaining 10 were used in a method involving the primary culture of the dispersed cells obtained from enzymatically disaggregated tissue. In vitro proliferation was analysed using optic microscopy, histological and histochemical techniques, and a scanning electron microscope. Cellular kinetics were also studied by bromodeoxyuridine marking. Using these tests it was found that it is possible to obtain the development and growth in this form of culture of fibroblastic-type colonies of epithelial origin characterised by different morphologies and growth curves. In addition, cells from the epithelioid colonies are characterised by a high level of proliferative activity and a low degree of differentiation. Mepartricina appears, on the other hand, to inhibit the in vitro growth of hypertrophic prostatic tissue.
Fandella
A
A
Divisione Urologica, USSL n. 10, Ospedale Civile Regionale, Treviso.
Merlo
F
F
De Angeli
S
S
D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D016591:Histocytological Preparation Techniques; D006801:Humans; D008297:Male; D008613:Mepartricin / Q000494:pharmacology*; D008954:Models, Biological; D011470:Prostatic Hyperplasia / Q000473:pathology*
D008613:Mepartricin