Isolation of a cDNA that encodes a novel granulocyte N-formyl peptide receptor.
A cDNA of 1650 base pairs was isolated by screening an HL-60 granulocyte library with an N-formyl peptide receptor (NFPR) cDNA probe under low stringency conditions. The cDNA encodes a protein of 351 amino acids tentatively named FPR2, with a calculated molecular weight of 39 kDa. Sequence analysis revealed that FPR2 is 69% identical in sequence to the human NFPR and shares extensive homology to several other chemoattractant receptors. FPR2 expressed in transfected cells mediated formyl peptide-stimulated calcium mobilization at micromolar concentrations of ligand. FPR2 messenger is detected in granulocytic HL-60 cells, but not in undifferentiated HL-60 cells. These findings suggest that FPR2 is a novel receptor for formyl peptide ligand and a new member of the chemoattractant receptor gene family.
Ye
R D
RD
Department of Immunology, Scripps Research Institute, La Jolla, CA 92037.
Cavanagh
S L
SL
Quehenberger
O
O
Prossnitz
E R
ER
Cochrane
C G
CG
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D015342:DNA Probes; D004273:DNA, Neoplasm / Q000235:genetics* / Q000302:isolation & purification; D006098:Granulocytes / Q000502:physiology*; D006801:Humans; D015473:Leukemia, Promyelocytic, Acute; D051379:Mice; D008969:Molecular Sequence Data; D008970:Molecular Weight; D005810:Multigene Family; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000378:metabolism; D011061:Poly A / Q000235:genetics / Q000302:isolation & purification; D016133:Polymerase Chain Reaction / Q000379:methods; D011487:Protein Conformation; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012333:RNA, Messenger; D044042:Receptors, Formyl Peptide; D011971:Receptors, Immunologic / Q000235:genetics* / Q000378:metabolism; D012689:Sequence Homology, Nucleic Acid; D014162:Transfection
D015342:DNA Probes; D004273:DNA, Neoplasm; D012333:RNA, Messenger; D044042:Receptors, Formyl Peptide; D011971:Receptors, Immunologic; D011061:Poly A; D009240:N-Formylmethionine Leucyl-Phenylalanine; D012313:RNA