[Measurement of the distance among CK-4, Hox 3.1 and Hox 3.3 genes on mouse chromosome 15 by pulse field gel electrophoresis].
Pulse field gel electrophoresis (PFGE) and Southern hybridization analysis were used to measure the maximum distance between the mouse type II cytokeratin-4 gene and the homeobox-containing gene Hox 3.3 to be 130kb, and that between Hox 3.3 and Hox 3.1 genes to be 50kb. Ck-4, Hox 3.1 and Hox 3.3 genes are mapped on mouse chromosome 15, and the order of loci is CK-4-Hox 3.3-Hox 3.1. A restriction map of Mlul, Notl, SacIl, Sal1 and Sfil restriction enzymes on a DNA fragment of 1,100 kb in length on which those three genes located was constructed.
Zhao
S
S
Institute of Genetics, Fudan University, Shanghai.
D000818:Animals; D015139:Blotting, Southern; D002874:Chromosome Mapping; D016521:Electrophoresis, Gel, Pulsed-Field; D005796:Genes; D005801:Genes, Homeobox; D007633:Keratins / Q000235:genetics*; D051379:Mice
D007633:Keratins