A new, simple myelin stain.
A new and convenient myelin stain is described. Paraformaldehyde fixed tissue is serially immersed in a nitroblue tetrazolium solution and then in a diaminobenzidine solution. The result is distinct blue staining of myelinated fiber tracts. This technique has advantages over presently used myelin stains.
Kaatz
K W
KW
Department of Neurology, University of Michigan, Ann Arbor 48109.
Bazzett
T J
TJ
Albin
R L
RL
D000818:Animals; D008297:Male; D009186:Myelin Sheath / Q000648:ultrastructure*; D009474:Neurons / Q000648:ultrastructure*; D009580:Nitroblue Tetrazolium; D051381:Rats; D017207:Rats, Sprague-Dawley; D013194:Staining and Labeling; D016707:Tissue Fixation
D009580:Nitroblue Tetrazolium