Comparison of L-selectin and E-selectin ligand specificities: the L-selectin can bind the E-selectin ligands sialyl Le(x) and sialyl Le(a).
The L- and E-selectins are leukocyte and endothelial cell surface molecules which mediate leukocyte-endothelial cell adhesion by interacting with carbohydrate ligands. In the present study we find that L-selectin, like E-selectin, can interact with synthetic neoglycoproteins containing Sialyl Le(x) (Neu5Ac alpha 2-3Gal beta 1-4[Fuc alpha 1-3]GlcNAc beta-R), or Sialyl Le(a) (Neu5Ac-alpha 2-3Gal beta 1-3[Fuc alpha 1-4]GlcNAc beta-R). Additionally, both the E-selectin and L-selectin can bind the peripheral lymph node addressin, a high endothelial venule ligand for L-selectin. Despite overlapping interactions, the L- and E-selectins discriminate between their native ligands. The peripheral lymph node addressin is a preferential ligand for L-selectin; and furthermore, L-selectin expressing cells do not interact detectably with the cutaneous lymphocyte antigen, a native glycoprotein ligand for E-selectin found on a subset of lymphocytes associated with the skin.
Berg
E L
EL
Department of Pathology, Stanford University, CA 94305.
Magnani
J
J
Warnock
R A
RA
Robinson
M K
MK
Butcher
E C
EC
D000818:Animals; D000911:Antibodies, Monoclonal; D018395:CA-19-9 Antigen; D002240:Carbohydrate Sequence; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D019040:E-Selectin; D005732:Gangliosides / Q000378:metabolism*; D006001:Glycoconjugates / Q000378:metabolism; D006023:Glycoproteins / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D019041:L-Selectin; D016256:Lewis X Antigen / Q000378:metabolism*; D008214:Lymphocytes / Q000276:immunology; D008562:Membrane Glycoproteins / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D011485:Protein Binding; D014162:Transfection
D000911:Antibodies, Monoclonal; D018395:CA-19-9 Antigen; D015815:Cell Adhesion Molecules; D019040:E-Selectin; D005732:Gangliosides; D006001:Glycoconjugates; D006023:Glycoproteins; D016256:Lewis X Antigen; D008562:Membrane Glycoproteins; D019041:L-Selectin; C064564:sialyl Le(a) ganglioside