title: Recognition of synthetic peptides with sequences of rubella virus E1 polypeptide by antibodies and T lymphocytes.
abstract: Antigenicity of rubella virus E1 polypeptide was analyzed using synthetic peptides with predicted amino acid sequences. Overlapping solid-phase bound peptides were used to define antibody binding domains and a panel of free peptides to study T-cell responsiveness. Several antibody-binding areas including those earlier described to contain major neutralizing epitopes were recognized by human sera positive for rubella antibodies. T-cell lines specific for rubella virus were established from 14 rubella immune subjects. All cell lines responded to rubella virion-derived antigen but only eight (57%) responded to one or more of the synthetic peptides. Individual patterns of peptide recognition were found but peptide 8 representing amino acids 402-422 was most often stimulatory to T-cells lines, either alone (3 subjects) or in combination with peptide 3 (amino acids 245-269) or 3 and 4 (amino acids 269-287). The response was HLA restricted but no single DR specificity for this restriction was identified.
authors.affiliation: Department of Virology, University of Turku, Finland.
authors.forename: J
authors.initials: J
authors.lastname: Ilonen
authors.forename: H
authors.initials: H
authors.lastname: Seppänen
authors.forename: A
authors.initials: A
authors.lastname: Närvänen
authors.forename: M
authors.initials: M
authors.lastname: Korkolainen
authors.forename: A A
authors.initials: AA
authors.lastname: Salmi
mesh_terms: D000914:Antibodies, Viral / Q000276:immunology*; D000956:Antigens, Viral / Q000276:immunology; D001666:Binding Sites, Antibody / Q000276:immunology; D002460:Cell Line; D002478:Cells, Cultured; D000939:Epitopes / Q000276:immunology; D006684:HLA-DR Antigens / Q000276:immunology; D006801:Humans; D007124:Immunoenzyme Techniques; D008213:Lymphocyte Activation / Q000276:immunology; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology*; D012412:Rubella virus / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*; D014759:Viral Envelope Proteins / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D000939:Epitopes; D006684:HLA-DR Antigens; D010455:Peptides; D014759:Viral Envelope Proteins; C061826:E1 envelope protein, Rubella virus