The cGMP-gated channel of rod outer segments is not localized in bipolar cells of the mammalian retina.
It has recently been suggested (Nature, 346 (1990) 269-271) that ON-bipolar cells express the same biochemical cascade and guanosine 3',5'-cyclic monophosphate (cGMP)-gated cation channel as rod outer segments. An antibody directed against the cGMP-gated channel of bovine rod outer segments was applied to cryostat sections of rat and cat retinae. No immunocytochemical labelling was found in bipolar cells. Therefore, if those cells express a cGMP-gated channel, it must be immunologically different to the 63 kDa protein constituting the cGMP-gated channel of the outer segment.
Wässle
H
H
Max-Planck-Institut für Hirnforschung, Frankfurt am Main, F.R.G.
Grünert
U
U
Cook
N J
NJ
Molday
R S
RS
D000818:Animals; D000911:Antibodies, Monoclonal; D002415:Cats; D006152:Cyclic GMP / Q000502:physiology*; D007150:Immunohistochemistry; D015640:Ion Channel Gating; D007473:Ion Channels / Q000502:physiology* / Q000648:ultrastructure; D008857:Microscopy, Interference; D011493:Protein Kinase C / Q000032:analysis; D051381:Rats; D012160:Retina / Q000166:cytology / Q000502:physiology*; D012374:Rod Cell Outer Segment / Q000502:physiology* / Q000648:ultrastructure
D000911:Antibodies, Monoclonal; D007473:Ion Channels; D011493:Protein Kinase C; D006152:Cyclic GMP