The serotyping of Azospirillum spp. by cell-gold immunoblotting.
Ten Azospirillum strains were serotyped using the method of cell-gold immunoblotting (dot-blot immune-overlay assay). Colloidal gold-protein A conjugate was used. Antibodies raised against the whole cells showed strain specificity and interacted mainly with carbohydrate antigens on the cell surface. Immunological identity for A. brasilense Sp 245 and Sp 107 strains was found. Cell-gold immunoblotting can be recommended for serotyping of a wide variety of bacterial strains.
Bogatyrev
V A
VA
Institute of Biochemistry and Physiology of Plants and Microorganisms, Academy of Sciences of Russia, Saratov.
Dykman
L A
LA
Matora LYu
Schwartsburd
B I
BI
D000942:Antigens, Bacterial / Q000302:isolation & purification; D016949:Azospirillum / Q000145:classification* / Q000276:immunology; D005069:Evaluation Studies as Topic; D006046:Gold; D015151:Immunoblotting / Q000379:methods*; D007122:Immunoelectrophoresis; D012703:Serotyping / Q000379:methods*; D013205:Staphylococcal Protein A
D000942:Antigens, Bacterial; D013205:Staphylococcal Protein A; D006046:Gold