Analysis of novel imidazoles from isolated perfused rabbit heart by two high-performance liquid chromatographic methods.
The paper reports two analytical high-performance liquid chromatographic methods to detect and quantify cardiac-derived histidyl derivatives. Method A relies on relative hydrophobicities as a basis of separation. Method B is an ion-pairing method in which the compounds are eluted in an entirely different order. Fractions collected from method A have been co-eluted in admixture in method B with authentic reference compounds. Thus the existence of the following imidazole ring-containing compounds derived from heart have been confirmed: N-acetylhistidine, N-acetyl-1-methylhistidine, N-acetylcarnosine, N-acetylhomocarnosine, homocarnosine, anserine, carnosine, balenine. Compounds were found in both tissue samples and perfusates.
O'Dowd
A
A
Institute of Physiology, Glasgow University, Scotland, UK.
O'Dowd
J J
JJ
O'Dowd
J J
JJ
MacFarlane
N
N
Abe
H
H
Miller
D J
DJ
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D007093:Imidazoles / Q000032:analysis*; D009206:Myocardium / Q000737:chemistry*; D010477:Perfusion; D011817:Rabbits; D013056:Spectrophotometry, Ultraviolet
D007093:Imidazoles