title: High-performance liquid chromatography and post-column derivatization with diphenyl-1-pyrenylphosphine for fluorimetric determination of triacylglycerol hydroperoxides.
abstract: Triacylglycerol monohydroperoxides (TG-mHPO) were selectively detected at the picomole levels after post-column reaction with diphenyl-1-pyrenylphosphine (DPPP). TG-mHPO were separated on two types of reserved-phase columns, an ODS column and a phenylated silica gel column, which were useful for determining TG-mHPO at their molecular species levels and their class levels, respectively. After the separation, DPPP solution was mixed with the eluent followed by reaction in a stainless-steel coil 20 m x 0.5 mm I.D. at 80 degrees C, then the fluorescence intensity of DPPP oxide was measured (lambda ex. 352 nm, lambda em. 380 nm). Using these systems, TG-mHPO were determined in the range 2-1000 pmol. The relative standard deviations were 2.3-2.8%.
authors.affiliation: Department of Food Chemistry, Faculty of Agriculture, Tohoku University, Sendai, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Akasaka
authors.forename: S
authors.initials: S
authors.lastname: Ijichi
authors.forename: K
authors.initials: K
authors.lastname: Watanabe
authors.forename: H
authors.initials: H
authors.lastname: Ohrui
authors.forename: H
authors.initials: H
authors.lastname: Meguro
mesh_terms: D002851:Chromatography, High Pressure Liquid; D005504:Food Analysis; D008054:Lipid Peroxides / Q000032:analysis*; D008832:Microchemistry; D009821:Oils / Q000032:analysis; D009943:Organophosphorus Compounds; D010938:Plant Oils / Q000032:analysis; D011721:Pyrenes; D013050:Spectrometry, Fluorescence; D014280:Triglycerides / Q000032:analysis*
chemical_list: D008054:Lipid Peroxides; D009821:Oils; D009943:Organophosphorus Compounds; D010938:Plant Oils; D011721:Pyrenes; D014280:Triglycerides; C066322:diphenyl-1-pyrenylphosphine