Frequency of T cells specific for myelin basic protein and myelin proteolipid protein in blood and cerebrospinal fluid in multiple sclerosis.
T cell sensitization to two myelin components, myelin basic protein (MBP) and myelin proteolipid protein (PLP), may be important to the pathogenesis of multiple sclerosis (MS). Using the limiting dilution assay, we demonstrated that the blood of MS patients had an increased frequency of MBP-reactive T cells compared with normal subjects and patients with other neurological diseases (OND) and rheumatoid arthritis. There was no difference in T cell frequency to a synthetic peptide, PLP139-151, or Herpes simplex virus. Within cerebrospinal fluid (CSF), 37% of IL-2/IL-4-reactive T cell isolates from MS patients responded either to MBP or PLP139-151 while only 5% of similar isolates from OND patients responded to these myelin antigens. The mean relative frequency of MBP-reactive T cells within CSF from MS patients was significantly higher than that of OND patients (22 x 10(-5) cells versus 1 x 10(-5) cells) and was similar to that of MBP reactive T cells within the central nervous system of rats with experimental autoimmune encephalomyelitis. These results lend new support to the hypothesis that myelin-reactive T cells mediate disease in MS.
Chou
Y K
YK
Neuroimmunology Research, V.A. Medical Center, Portland, OR 97201.
Bourdette
D N
DN
Offner
H
H
Whitham
R
R
Wang
R Y
RY
Hashim
G A
GA
Vandenbark
A A
AA
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000737:chemistry / Q000494:pharmacology; D001773:Blood Cells / Q000276:immunology*; D002555:Cerebrospinal Fluid / Q000166:cytology / Q000276:immunology*; D006801:Humans; D007201:Indicator Dilution Techniques; D007376:Interleukin-2 / Q000134:cerebrospinal fluid; D015847:Interleukin-4 / Q000134:cerebrospinal fluid; D007958:Leukocyte Count; D008969:Molecular Sequence Data; D009103:Multiple Sclerosis / Q000097:blood / Q000134:cerebrospinal fluid / Q000276:immunology*; D004676:Myelin Basic Protein / Q000276:immunology*; D009185:Myelin Proteins / Q000276:immunology*; D018991:Myelin Proteolipid Protein; D013601:T-Lymphocytes / Q000276:immunology*
D000596:Amino Acids; D007376:Interleukin-2; D004676:Myelin Basic Protein; D009185:Myelin Proteins; D018991:Myelin Proteolipid Protein; D015847:Interleukin-4