title: Two chicken monoclonal antibodies specific for heterophil Hanganutziu-Deicher antigens.
abstract: Two chicken monoclonal antibodies (MAbs), HU/Ch2-7 and HU/Ch6-1, against heterophil Hanganutziu-Deicher (HD) antigens with N-glycolylneuraminic acid (NeuGc) at a terminal carbohydrate were established by cell fusions using chicken B cell lines lacking thymidine kinase and spleen cells from chickens immunized with II3NeuGc alpha-LacCer (HD3). The reactivities of these MAbs against several gangliosides including NeuGc-containing glycosphingolipids were examined by a thin-layer chromatography/immunostaining method. MAb HU/Ch2-7 (IgG) reacted strongly with HD3 and IV3NeuGc alpha-nLc4Cer (HD5) and weakly with VI3NeuGc alpha-nLc6Cer (HD7) and 4-O-acetyl-HD3. HU/Ch6-1 (IgG) reacted with HD3 and HD5, but did not react with the other HD antigens. The reactivities of these MAbs against HD antigen were greatly reduced by pre-treatment of the antigen with neuraminidase. These MAbs did not react with N-acetylneuraminic acid-containing gangliosides (GM1 and GM3). These results indicate that these two chicken MAbs are directed toward the antigenic epitope containing the NeuGc.
authors.affiliation: Department of Microbiology and Hygiene, Faculty of Applied Biological Science, Hiroshima University, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Asaoka
authors.forename: S
authors.initials: S
authors.lastname: Nishinaka
authors.forename: N
authors.initials: N
authors.lastname: Wakamiya
authors.forename: H
authors.initials: H
authors.lastname: Matsuda
authors.forename: M
authors.initials: M
authors.lastname: Murata
mesh_terms: D000818:Animals; D000910:Antibodies, Heterophile / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D015478:Antigens, Heterophile / Q000187:drug effects / Q000276:immunology*; D002240:Carbohydrate Sequence; D002645:Chickens / Q000276:immunology*; D000939:Epitopes / Q000276:immunology; D006825:Hybridomas / Q000276:immunology; D008969:Molecular Sequence Data; D009438:Neuraminic Acids / Q000276:immunology; D009439:Neuraminidase / Q000494:pharmacology
chemical_list: D000910:Antibodies, Heterophile; D000911:Antibodies, Monoclonal; D015478:Antigens, Heterophile; D000939:Epitopes; D009438:Neuraminic Acids; C032592:N-glycolylneuraminic acid; D009439:Neuraminidase