Sequence and expression of a rat cDNA for LECAM-1.
A rat cDNA clone encoding an adhesion molecule, LECAM-1, has been isolated from the SD rat and the partial nucleotide sequence was determined. It encodes a peptide of 372 amino acids (aa), including a signal peptide of 38 aa. The protein has three tandem domains: a lectin domain, an EGF-like domain and two repeats of complement regulatory proteins (CR domain). The lectin binding domain has 93.2% and 81.4% and the EGF-like domain has 85.3% and 76.5% aa identity with those of mouse and human, respectively. In the CR repeat domain, the amino acid identity was 72.6% between human and rat and 71.8% between mouse and rat. Northern blot analysis detects the main transcript of about 3 kb in peripheral blood mononuclear cells (PBMC), spleen and thymus. The expression was down-regulated by mitogen stimulation of PBMC and spleen T cells. The protein encoded by this cDNA interacted with PPME when it was expressed on gp90MEL-14 negative mouse EL-4 cells.
Watanabe
T
T
Department of Pathology, University of Tokyo, Japan.
Song
Y
Y
Hirayama
Y
Y
Tamatani
T
T
Kuida
K
K
Miyasaka
M
M
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015815:Cell Adhesion Molecules / Q000737:chemistry / Q000235:genetics*; D003001:Cloning, Molecular; D015536:Down-Regulation / Q000502:physiology; D019041:L-Selectin; D007963:Leukocytes, Mononuclear; D008213:Lymphocyte Activation / Q000235:genetics; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D051381:Rats; D012689:Sequence Homology, Nucleic Acid; D013601:T-Lymphocytes
D015815:Cell Adhesion Molecules; D008562:Membrane Glycoproteins; D019041:L-Selectin