Characterization of the chicken alpha 1(VI) collagen promoter.
The promoter of the chicken alpha 1(VI) collagen gene resembles the 5'-flanking regions of many housekeeping genes. It lacks a canonical TATAA box but contains potential binding sites for transcription factors AP1 and SP1. The promoter region has a relatively high GC content and forms a typical CpG island. In accordance with the absence of a TATAA element, the gene contains multiple transcription-initiation sites distributed over 80 bp genomic DNA. A 621-bp fragment derived from the 5' end of the alpha 1(VI) collagen gene is able to direct transcription of a heterologous reporter gene in transient-expression assays. Other DNA fragments that are either shorter or longer than the 621-bp fragment show markedly reduced promoter activity. Thus, the basic promoter element of the alpha 1(VI) collagen gene must reside within this 621-bp fragment.
Koller
E
E
Laboratorium für Biochemie I, Eidgenössische Technische Hochschule, Zürich, Switzerland.
Trueb
B
B
D000818:Animals; D001483:Base Sequence; D002645:Chickens; D003094:Collagen / Q000235:genetics*; D005786:Gene Expression Regulation; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012045:Regulatory Sequences, Nucleic Acid; D015183:Restriction Mapping; D013329:Structure-Activity Relationship; D014158:Transcription, Genetic
D003094:Collagen