Galanin inhibits rat pancreatic amylase release via cholinergic suppression.
The effects of galanin on pancreatic exocrine function were examined using rat pancreatic tissues. In anesthetized rats, galanin (40 micrograms/kg/h) decreased amylase secretion stimulated by 2-deoxy glucose (5.8 +/- 0.1 vs. 3.1 +/- 0.1 times basal) and cholecystokinin octapeptide (21.5 +/- 0.6 vs. 16.8 +/- 0.5), while not inhibiting bethanechol-stimulated secretion. In dispersed acini, there was no effect of galanin alone (10(-8) to 10(-13) M) on amylase release, nor did galanin (10(-6) or 10(-8) M) coincubation affect amylase release stimulated by bethanechol (10(-3) to 10(-7) M) or CCK-8 (10(-8) to 10(-13) M). Using pancreatic lobules, coincubation with galanin (10(-6) M) suppressed 75 mM KCl-stimulated amylase secretion and ACh release (10.1 +/- 0.6% vs. 7.3 +/- 0.4%). Veratridine-stimulated (10(-4) M) amylase secretion and ACh release (12.4 +/- 1.7% vs. 8.5 +/- 0.7%) were similarly diminished.
Flowe
K M
KM
Department of Surgery, University of Michigan Medical Center, Ann Arbor 48109.
Lally
K M
KM
Mulholland
M W
MW
D000109:Acetylcholine / Q000378:metabolism*; D000818:Animals; D018723:Bethanechol; D001626:Bethanechol Compounds / Q000494:pharmacology; D003847:Deoxyglucose / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D019004:Galanin; D066298:In Vitro Techniques; D008297:Male; D009479:Neuropeptides / Q000494:pharmacology*; D010179:Pancreas / Q000187:drug effects / Q000378:metabolism*; D010455:Peptides / Q000494:pharmacology*; D011189:Potassium Chloride / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D012844:Sincalide / Q000494:pharmacology; D014701:Veratridine / Q000494:pharmacology; D000516:alpha-Amylases / Q000187:drug effects / Q000378:metabolism*
D001626:Bethanechol Compounds; D009479:Neuropeptides; D010455:Peptides; D018723:Bethanechol; D011189:Potassium Chloride; D014701:Veratridine; D019004:Galanin; D003847:Deoxyglucose; D000516:alpha-Amylases; D012844:Sincalide; D000109:Acetylcholine