Collaborative study of mutagenicity with Salmonella typhimurium TA102.
Thirty compounds of various chemical classes were investigated for mutagenicity in a collaborative study (3 laboratories) using Salmonella typhimurium TA102. With 5 compounds, namely hydrazine sulfate, phenylhydrazine, hydralazine, glutardialdehyde and glyoxal, mutagenicity was detected by all laboratories. Formaldehyde was assessed as weakly mutagenic in only 1 of 3 laboratories. The remaining 24 agents were uniformly described as non-genotoxic in TA102. In spite of the overall good qualitative agreement in the mutagenicity results between the 3 laboratories some quantitative discrepancies occurred in the dose response of the mutagenic compounds. Varying inter- and intra-laboratory differences in the spontaneous rate of revertants were obtained. The usefulness of the tester strain TA102 in routine mutagenicity testing is discussed.
Jung
R
R
Department of Toxicology, Hoechst AG, Frankfurt, Germany.
Engelhart
G
G
Herbolt
B
B
Jäckh
R
R
Müller
W
W
D005069:Evaluation Studies as Topic; D005976:Glutaral / Q000633:toxicity; D006037:Glyoxal / Q000633:toxicity; D006830:Hydralazine; D006834:Hydrazines / Q000633:toxicity; D009152:Mutagenicity Tests / Q000379:methods / Q000592:standards; D009153:Mutagens / Q000633:toxicity; D010659:Phenylhydrazines / Q000633:toxicity; D012486:Salmonella typhimurium / Q000187:drug effects*
D006834:Hydrazines; D009153:Mutagens; D010659:Phenylhydrazines; C030299:phenylhydrazine; D006830:Hydralazine; C029424:hydrazine; D006037:Glyoxal; D005976:Glutaral