title: Characterization of the plasmid mediated beta-lactamase BIL-1.
abstract: A multi-resistant strain of Escherichia coli isolated from a patient from Pakistan was shown to possess a new plasmid-mediated beta-lactamase. This enzyme, designated BIL-1, conferred resistance to extended-spectrum cephalosporins such as cefotaxime and ceftazidime. However, unlike other plasmid-mediated beta-lactamases capable of conferring resistance to these drugs, the BIL-1 was not a member of the TEM or SHV group of plasmid-mediated beta-lactamases and it also conferred resistance to beta-lactam drugs in combination with beta-lactamase inhibitors (i.e. clavulanic acid). The biochemical properties of the enzyme suggest that BIL-1 is related to the Richmond & Sykes Class I chromosomal beta-lactamases. Its inhibition properties by various beta-lactam drugs are similar to the inhibition properties of the chromosomally-encoded P99 enzyme of Enterobacter cloacae.
authors.affiliation: Department of Medical Microbiology, University Medical School, Edinburgh, UK.
authors.forename: D J
authors.initials: DJ
authors.lastname: Payne
authors.forename: N
authors.initials: N
authors.lastname: Woodford
authors.forename: S G
authors.initials: SG
authors.lastname: Amyes
mesh_terms: D000900:Anti-Bacterial Agents / Q000378:metabolism / Q000494:pharmacology*; D002511:Cephalosporins / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology*; D007525:Isoelectric Focusing; D008826:Microbial Sensitivity Tests; D010957:Plasmids; D065093:beta-Lactamase Inhibitors; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D002511:Cephalosporins; D065093:beta-Lactamase Inhibitors; D001618:beta-Lactamases