[Study on localization of cystic fluid antigen of Cysticercus cellulosae using McAb-IGSS].
A study was undertaken on the localization of specific combination of McAb against cystic fluid antigen of Cysticercus cellulosae by immunogold silver staining method (IGSS). It was shown that the combination sites of McAb F3 were distinct in the frozen and paraffin sections of Cysticercus cellulosae. The black granules in the bladder walls presented double-layer distribution and those in the scolex folded sites concentrated into pieces. The combination sites of McAb labeled by IGSS against cystic fluid antigen of Cysticercus cellulosae were consistent with the distribution of the cell layers of Cysticercus cellulosae. The sections in the control group had scarce and irregular distribution, which could be differentiated distinctly from those in the experimental group.
Huang
N Z
NZ
Department of Parasitology, Nanjing Railway Medical College.
Yang
H Y
HY
Wang
X H
XH
Xu
Z J
ZJ
Chen
A H
AH
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000947:Antigens, Helminth / Q000032:analysis*; D003552:Cysticercus / Q000033:anatomy & histology / Q000276:immunology*; D007150:Immunohistochemistry
D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth