Evaluation and optimization of a latex agglutination assay for detection of cholera toxin and Escherichia coli heat-labile toxin.
The effectiveness of a latex agglutination assay kit for the detection of Escherichia coli heat-labile toxin and cholera toxin was determined for the identification of natural isolates of the corresponding enteric pathogens in Southeast Asia. By selection of the appropriate culture media, the sensitivity of the assay was improved from 90.6% (for the detection of heat-labile toxin) and 75% (for the detection of cholera toxin) to 100%, and the results were confirmed with bioassays and DNA hybridization assays for both clinical and environmental isolates.
Yam
W C
WC
Department of Microbiology, University of Hong Kong.
Lung
M L
ML
Ng
M H
MH
D000372:Agglutination Tests / Q000379:methods*; D001427:Bacterial Toxins / Q000032:analysis* / Q000276:immunology; D001681:Biological Assay; D002772:Cholera Toxin / Q000032:analysis* / Q000276:immunology; D004768:Enterotoxins / Q000032:analysis* / Q000276:immunology; D029968:Escherichia coli Proteins; D005069:Evaluation Studies as Topic; D007118:Immunoassay / Q000379:methods*; D008863:Microspheres; D009693:Nucleic Acid Hybridization; D012680:Sensitivity and Specificity
D001427:Bacterial Toxins; D004768:Enterotoxins; D029968:Escherichia coli Proteins; D002772:Cholera Toxin; C027538:heat-labile enterotoxin, E coli