Cross-linking activity of the 14-kilodalton beta-galactoside-specific vertebrate lectin with asialofetuin: comparison with several galactose-specific plant lectins.
We have previously shown that plant lectins with a wide range of carbohydrate binding specificities can bind and cross-link (precipitate) specific multiantennary oligosaccharides and glycopeptides [cf. Bhattacharyya, L., Fant, J., Lonn, H., & Brewer, C. F. (1990) Biochemistry 29, 7523-7530]. This leads to a new source of binding specificity: namely, the formation of homogeneous cross-linked lattices between lectins and carbohydrates. Recently, we have demonstrated the existence of highly ordered cross-linked lattices that form between the D-Man/D-Glc-specific plant lectin concanavalin A and the soybean agglutinin which is a tetrameric glycoprotein possessing a single Man9 oligomannose chain per monomer [Khan, M. I., Mandal, D. K., & Brewer, C. F. (1991) Carbohydr. Res. 213, 69-77]. In the present study, we have compared the ability of the 14-kDa beta-galactoside-specific lectin from calf spleen, a dimeric S-type animal lectin, and several galactose-specific plant lectins from Erythrina indica, Erythrina cristagalli, and Glycine max (soybean agglutinin) to form specific cross-linked complexes with asialofetuin (ASF), a 48-kDa monomeric glycoprotein, using quantitative precipitation analyses. The results show the formation of 1:9 and 1:3 stoichiometric cross-linked complexes (per monomer) of ASF to the 14-kDa lectin, depending on their relative ratio in solution. Evidence indicates that the three triantennary N-linked complex-type oligosaccharide chains of ASF mediate the cross-linking interactions and that each chain expresses either trivalency in the 1:9 cross-linked complex or univalency in the 1:3 complex.(ABSTRACT TRUNCATED AT 250 WORDS)
Mandal
D K
DK
Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, New York 10461.
Brewer
C F
CF
D000818:Animals; D001212:Asialoglycoproteins / Q000378:metabolism*; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002417:Cattle; D011232:Chemical Precipitation; D003432:Cross-Linking Reagents; D060748:Fetuins; D005697:Galactosides / Q000378:metabolism*; D037102:Lectins / Q000378:metabolism*; D046911:Macromolecular Substances; D008961:Models, Structural; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000378:metabolism; D037121:Plant Lectins; D010944:Plants / Q000737:chemistry; D013154:Spleen / Q000737:chemistry*; D013379:Substrate Specificity; D000509:alpha-Fetoproteins / Q000378:metabolism*
D001212:Asialoglycoproteins; D003432:Cross-Linking Reagents; D060748:Fetuins; D005697:Galactosides; D037102:Lectins; D046911:Macromolecular Substances; D009844:Oligosaccharides; D037121:Plant Lectins; D000509:alpha-Fetoproteins; C017029:asialofetuin; C045629:erythrina lectin