title: Detection of tobacco rattle virus by reverse transcription and polymerase chain reaction.
abstract: An assay involving reverse transcription followed by polymerase chain reaction specifically detected tobacco rattle virus RNA in extracts of infected plants. It detected a wide range of serological variants and typical non-particle producing NM-type isolates. The sensitivity of the method was sufficient to detect TRV RNA in 10 ng total nucleic acid from an infected plant, or in a relatively crude nucleic acid preparation from 60 micrograms infected leaf tissue.
authors.affiliation: Scottish Crop Research Institute, Invergowrie, Dundee, UK.
authors.forename: D J
authors.initials: DJ
authors.lastname: Robinson
mesh_terms: D001483:Base Sequence; D008969:Molecular Sequence Data; D010942:Plant Viruses / Q000235:genetics / Q000302:isolation & purification*; D010944:Plants / Q000382:microbiology*; D010947:Plants, Toxic; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000032:analysis* / Q000235:genetics; D012194:RNA-Directed DNA Polymerase; D012680:Sensitivity and Specificity; D014026:Nicotiana / Q000382:microbiology*; D014158:Transcription, Genetic
chemical_list: D012367:RNA, Viral; D012194:RNA-Directed DNA Polymerase