Chemiluminescent universal probe for bacterial ribotyping.
We describe a novel procedure for direct covalent coupling of horseradish peroxidase to rRNA and ribotyping by using enhanced chemiluminescence. Compared with their 32P-end-labeled counterparts, chemiluminescent rRNA probes are stable and easy to synthesize and provide results as good as or superior to those obtained with isotopic labeling. Direct chemiluminescent labeling of Escherichia coli rRNA produces a sensitive, universal probe suitable for clinical laboratory use in the investigation of nosocomial outbreaks.
Gustaferro
C A
CA
Department of Internal Medicine, Mayo Clinic, Rochester, Minnesota 55905.
Persing
D H
DH
D015373:Bacterial Typing Techniques; D004926:Escherichia coli / Q000235:genetics; D005069:Evaluation Studies as Topic; D008163:Luminescent Measurements; D015336:Molecular Probe Techniques; D015347:RNA Probes; D012329:RNA, Bacterial / Q000235:genetics; D012335:RNA, Ribosomal / Q000235:genetics
D015347:RNA Probes; D012329:RNA, Bacterial; D012335:RNA, Ribosomal