Identification of N-acetylneuraminyl alpha 2-->3 poly-N-acetyllactosamine glycans as the receptors of sialic acid-binding Streptococcus suis strains.
Streptococcus suis is a common cause of sepsis, meningitis, and other serious infections in young piglets and also causes meningitis in humans. The cell-binding specificity of sialic acid-recognizing strains of Streptococcus suis was investigated. Treatment of human erythrocytes with sialidase or mild periodate abolished hemagglutination. Hemagglutination inhibition experiments with sialyl oligosaccharides indicated that the adhesin preferred the sequence NeuNAc alpha 2-3Gal beta 1-4Glc(NAc). Resialylation of desialylated erythrocytes with Gal beta 1-3(4)GlcNAc alpha 2-3-sialyltransferase induced a strong hemagglutination, whereas no or only weak hemagglutination was obtained with cells resialylated with two other sialyltransferases. Binding of radiolabeled bacteria to blots of erythrocyte membrane proteins revealed binding to the poly-N-acetyllactosamine-containing components Band 3, Band 4.5, and polyglycosyl ceramides and to glycophorin A. The involvement of glycophorin A as a major ligand was excluded by the strong hemagglutination of trypsin-treated erythrocytes and En(a-) erythrocytes defective in glycophorin A. Sensitivity of the hemagglutination toward endo-beta-galactosidase treatment of erythrocytes and inhibition by purified poly-N-acetyllactosaminyl glycopeptides indicated that the adhesin bound to glycans containing the following structure: NeuNAc alpha 2-3Gal beta 1-4GlcNAc beta 1-3Gal beta 1-.
Liukkonen
J
J
Department of Biochemistry and Biotechnology, University of Kuopio, Finland.
Haataja
S
S
Tikkanen
K
K
Kelm
S
S
Finne
J
J
D018829:Adhesins, Bacterial; D000328:Adult; D000818:Animals; D001426:Bacterial Proteins / Q000378:metabolism*; D001665:Binding Sites; D002240:Carbohydrate Sequence; D004912:Erythrocytes / Q000502:physiology; D006384:Hemagglutination / Q000187:drug effects; D006386:Hemagglutination Tests; D006801:Humans; D007231:Infant, Newborn; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000378:metabolism* / Q000494:pharmacology; D011134:Polysaccharides / Q000737:chemistry / Q000378:metabolism*; D011971:Receptors, Immunologic / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D012794:Sialic Acids / Q000378:metabolism*; D012799:Sialyltransferases / Q000302:isolation & purification / Q000378:metabolism; D017011:Streptococcus suis / Q000302:isolation & purification / Q000502:physiology*; D013379:Substrate Specificity; D013552:Swine
D018829:Adhesins, Bacterial; D001426:Bacterial Proteins; D009844:Oligosaccharides; D011134:Polysaccharides; D011971:Receptors, Immunologic; D011994:Recombinant Proteins; D012794:Sialic Acids; C016735:sialooligosaccharides; D012799:Sialyltransferases