Shift of private and not of cross-reactive anti-DNA idiotypes in systemic lupus erythematosus.
The shift of private idiotype (Id) and cross-reactive Id (CRI) on anti-DNA antibodies in a lupus patient KE was investigated during a 7-year period. Anti-private Id and anti-CRI activities were separated by affinity chromatography from rabbit (R)-anti-Ids raised against KE anti-DNA antibodies during active (1/84) and inactive (4/90) stages of the disease. Anti-CRI isolated from the 84 R-anti-Id appeared to recognize binding site-related Ids that are shared with KE non-anti-DNA antibodies, unrelated lupus patients' sera, and certain normal sera. Id expression on serial serum samples of KE using these fractionated R-anti-Ids as probes showed that the 1/84 private Id expression declined while the 4/90 private Id expression gradually increased. Expression of the CRI showed a relatively stable pattern. These results suggest that anti-DNA populations detected by anti-private Id can shift, while populations expressing CRI may stay stable.
Suenaga
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Immunology Research Laboratory, St. Luke's Hospital, Kansas City, Missouri 64111.
Abdou
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D000974:Antibodies, Antinuclear / Q000276:immunology*; D000939:Epitopes / Q000097:blood; D006801:Humans; D007130:Immunoglobulin Idiotypes / Q000097:blood / Q000276:immunology; D008180:Lupus Erythematosus, Systemic / Q000276:immunology*
D000974:Antibodies, Antinuclear; D000939:Epitopes; D007130:Immunoglobulin Idiotypes