Analysis of lymphocyte phenotype and T cell receptor genotype in Felty's syndrome.
Natural killer (NK) cells and CD3+ large granular lymphocytes (LGL) were investigated in patients with Felty's syndrome (FS), rheumatoid arthritis (RA) and healthy controls. In most patients with FS, NK cell number and activity were decreased. CD3+ LGL were unchanged. However, in one patient a marked expansion of CD3- CD16+ CD56+ (NK) cells was seen and in a second, an expansion of CD3+ LGL. In FS there was also an increase in HLA- DR+ and CD8+ but not gamma delta+ T cells. Three of 11 patients with FS studied demonstrated a dominant rearrangement of the T cell receptor beta gene constant region consistent with oligoclonal T cell expansion.
Meliconi
R
R
Department of Medicine, United Medical School, Guy's Hospital, London, United Kingdom.
Kingsley
G H
GH
Pitzalis
C
C
Sakkas
L
L
Panayi
G S
GS
D000368:Aged; D015703:Antigens, CD / Q000032:analysis; D000943:Antigens, Differentiation / Q000032:analysis; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis; D001172:Arthritis, Rheumatoid / Q000235:genetics / Q000276:immunology / Q000473:pathology; D017252:CD3 Complex; D018956:CD5 Antigens; D016827:CD8 Antigens / Q000032:analysis; D005258:Felty Syndrome / Q000235:genetics* / Q000276:immunology* / Q000473:pathology; D005260:Female; D015321:Gene Rearrangement; D005838:Genotype; D006684:HLA-DR Antigens / Q000032:analysis; D006801:Humans; D007694:Killer Cells, Natural / Q000276:immunology / Q000473:pathology / Q000648:ultrastructure; D008214:Lymphocytes / Q000276:immunology* / Q000473:pathology / Q000648:ultrastructure; D008297:Male; D008875:Middle Aged; D010641:Phenotype; D011948:Receptors, Antigen, T-Cell / Q000032:analysis / Q000235:genetics*; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000032:analysis / Q000235:genetics; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000032:analysis / Q000235:genetics; D011961:Receptors, Fc / Q000032:analysis; D017452:Receptors, IgG
D015703:Antigens, CD; D000943:Antigens, Differentiation; D000945:Antigens, Differentiation, T-Lymphocyte; D017252:CD3 Complex; D018956:CD5 Antigens; D016827:CD8 Antigens; D006684:HLA-DR Antigens; D011948:Receptors, Antigen, T-Cell; D016693:Receptors, Antigen, T-Cell, alpha-beta; D016692:Receptors, Antigen, T-Cell, gamma-delta; D011961:Receptors, Fc; D017452:Receptors, IgG