Primary B-cell response to neuropeptide Y and bovine pancreatic polypeptide.
An analysis of the murine primary response to protein epitopes has been made with two small highly structured proteins, neuropeptide Y (NPY) and bovine pancreatic polypeptide (BPP), both of 36-amino acid residue length and containing helical structures. A group of cell lines producing monoclonal IgM antibody have been prepared consisting of six anti-NPY and two anti-BPP. The VH nucleotide sequences have been determined and characterized as germ-line either by identity to established germ-line sequences or by inference from the germ-line character of the D and JH segments. The intrinsic association constants for the homologous ligands have been estimated to range from 10(4) to 10(7) M-1 based on competitive ELISA. No severe restriction in the utilization of VH families, D segments or JH segments appears to be involved in this response. Among the eight cell lines, three VH families were represented as well as all three families of D segments and all of the JH segments, although some preference for JH3 was indicated. The length of the N(D)N sequences was also not subject to restriction, ranging from 9 to 29. Two unusual features of the CRD3s were noted, one involving the utilization of an uncommon DSP2 segment and the other the apparent occurrence of a D-D fusion.
Deng
Y J
YJ
Department of Microbiology, School of Medicine, University of Pennsylvania, Philadelphia 19104.
Chua
M M
MM
Andrews
G C
GC
Karush
F
F
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000235:genetics; D001402:B-Lymphocytes / Q000276:immunology*; D001483:Base Sequence; D002417:Cattle; D003001:Cloning, Molecular; D000939:Epitopes; D015326:Gene Rearrangement, B-Lymphocyte, Heavy Chain; D005803:Genes, Immunoglobulin; D006825:Hybridomas; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D007135:Immunoglobulin Variable Region / Q000235:genetics; D008969:Molecular Sequence Data; D009154:Mutation; D009478:Neuropeptide Y / Q000138:chemical synthesis / Q000276:immunology*; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D010191:Pancreatic Polypeptide / Q000138:chemical synthesis / Q000276:immunology*; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment
D000911:Antibodies, Monoclonal; D000939:Epitopes; D007143:Immunoglobulin Heavy Chains; D007135:Immunoglobulin Variable Region; D009478:Neuropeptide Y; D009838:Oligodeoxyribonucleotides; D010191:Pancreatic Polypeptide