An immunohistochemical study on neurons and paraneurons of the pre- and post-natal chicken lung.
The indirect immunoperoxidase (PAP) method was used on chicken lung specimens from embryos ranging in age from 6 days to hatching, chicks and adult chickens of up to 6 months. The ontogenesis and distribution of neurons and paraneurons containing immunoreactivities for serotonin (5HT), bombesin, vasoactive intestinal polypeptide (VIP), substance P (SP) and galanin were investigated. Serotonin-immunoreactive paraneurons were first detected in the pulmonary mesenchyma of 8-day-old embryos, while in the 12-day-old embryos the following neurons and paraneurons were first detected in their respective locations: serotonin-immunoreactive paraneurons in the bronchial epithelium; VIP- and galanin-immunoreactive ganglionic cells and SP-immunoreactive nerve fibres in the intrapulmonary ganglia. At hatching, serotonin-immunoreactive paraneurons in the epithelium of the air capillaries and air sacs, and bombesin-immunoreactive paraneurons in the epithelium of the primary bronchus, VIP-, galanin- and SP-immunoreactive nerve fibres in the lamina propria of the primary and secondary bronchi and in the pulmonary septa could also be shown. Some serotonin-immunoreactive small paraneurons were also found in the intrapulmonary ganglia. In the adult specimens, VIP-, galanin- and SP-immunoreactive nerve fibre networks were observed throughout the primary bronchus wall and in the lung septa. In intrapulmonary ganglia, VIP- and galanin-immunoreactive neurons and serotonin-immunoreactive small paraneurons could be more numerously demonstrated. Moreover, bombesin paraneurons occurred in the epithelium of primary and secondary bronchi, and serotonin-immunoreactive paraneurons were found in the epithelia of the bronchi and air sacs and in some pluricellular bodies in the lamina propria of the air sac ostia.
Salvi
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Institute of Human Anatomy, University La Sapienza, Rome, Italy.
Renda
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D000818:Animals; D001839:Bombesin / Q000032:analysis; D002642:Chick Embryo; D002645:Chickens; D019004:Galanin; D005724:Ganglia / Q000737:chemistry*; D007150:Immunohistochemistry; D008168:Lung / Q000196:embryology / Q000254:growth & development / Q000294:innervation*; D009474:Neurons / Q000737:chemistry*; D010455:Peptides / Q000032:analysis; D012701:Serotonin / Q000032:analysis; D014660:Vasoactive Intestinal Peptide / Q000032:analysis
D010455:Peptides; D012701:Serotonin; D014660:Vasoactive Intestinal Peptide; D019004:Galanin; D001839:Bombesin