Suppression of immune responses by cloned T cells and their products.
We have established a number of IL-2 dependent T cell clones with a definite suppressor function for the antibody response. Our collection included CD4+ helper (Th) and suppressor T cell (CD4+ Ts), and CD8+ cytotoxic (Tc) and suppressor T cell (CD8+ Ts) clones. Ts clones were defined by their suppressive activity on antigen-induced proliferative response and on helper functions of other CD4+ Th clones without detectable cytotoxicity and production of known helper type interleukins. The I-J expression was found to be present on both Th and Ts clones, and was generally associated with the H-2 restriction specificity of TcR of these clones. We have shown that the activated CD4+ Ts cells could inhibit the increase of intracellular Ca2+ of Th clones induced by antigen-pulsed antigen-presenting cells (APC). The identity of MHC restriction-specificities was required for the instant suppression of Ca2+ influx of Th clones, while a longer period of activation of Ts clones was needed to suppress the response of Th clones having different MHC restrictions. A strict selectivity was found in the suppressive activity of Ts clones in that Ts clones could suppress the Ca2+ responses of Th1 or Th2 clones but not of other CD4+ Ts clones. Ts clones could suppress the Ca2+ influx of Th clones induced only by antigen-pulsed APC or anti-TcR antibody but not by Con A or IL-2. Both CD4+ and CD8+ Ts clones released soluble immunosuppressive factors upon stimulation with immobilized anti-CD3 antibody.(ABSTRACT TRUNCATED AT 250 WORDS)
Tada
T
T
Department of Immunology, Faculty of Medicine, University of Tokyo, Japan.
Inoue
T
T
Asano
Y
Y
D000818:Animals; D000917:Antibody Formation; D015704:CD4 Antigens / Q000276:immunology; D016827:CD8 Antigens / Q000276:immunology; D002999:Clone Cells; D016207:Cytokines / Q000032:analysis / Q000096:biosynthesis; D006801:Humans; D007376:Interleukin-2 / Q000276:immunology; D008285:Major Histocompatibility Complex; D016176:T-Lymphocyte Subsets / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology
D015704:CD4 Antigens; D016827:CD8 Antigens; D016207:Cytokines; D007376:Interleukin-2