title: Genomic organization and full-length cDNA sequence of human collagen X.
abstract: We have determined the full-length cDNA sequence of the human alpha 1(X) collagen gene by sequence analysis of a genomic clone ERG [(1991) Dev. Biol. 148, 562-572], and of cDNA fragments generated from a reverse transcribed as alpha 1(X) mRNA by PCR. We defined the promoter region, the transcription initiation site and the full-length 5'-untranslated region. We also report the exon/intron boundaries of the transcript and the complete 3'-untranslated region as well as a 3'-flanking sequence containing two additional polyadenylation signals. The promoter region is homologous to chicken and mouse type X promoters within several highly conserved regions. The genomic organization shows high homologies to chicken and mouse.
authors.affiliation: Max-Planck Society, University of Erlangen-Nürnberg, Germany.
authors.forename: E
authors.initials: E
authors.lastname: Reichenberger
authors.forename: F
authors.initials: F
authors.lastname: Beier
authors.forename: P
authors.initials: P
authors.lastname: LuValle
authors.forename: B R
authors.initials: BR
authors.lastname: Olsen
authors.forename: K
authors.initials: K
authors.lastname: von der Mark
authors.forename: W M
authors.initials: WM
authors.lastname: Bertling
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002645:Chickens; D003094:Collagen / Q000235:genetics*; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D015698:Genomic Library; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D016133:Polymerase Chain Reaction; D012045:Regulatory Sequences, Nucleic Acid; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
chemical_list: D009838:Oligodeoxyribonucleotides; D003094:Collagen; D004247:DNA