The structure of the O-specific polysaccharide chain of the lipopolysaccharide of Salmonella arizonae O61.
The O-specific polysaccharide was obtained by mild degradation of the Salmonella arizonae O61 lipopolysaccharide with acid. It contained 2-acetamido-2-deoxy-D-glucose, 2-acetamidino-2,6-dideoxy-L-galactose (FucAm), and 7-acetamido-3,5,7,9-tetradeoxy-5-[(R)-3-hydroxybutyramido]-D- glycero-L-galacto-nonulosonic acid (Sug). On the basis of partial acid hydrolysis with 0.1 M HCl, solvolysis with anhydrous HF in methanol, and 1H- and 13C-NMR analysis (including 1H/13C inversely correlated spectroscopy for localisation of N-acyl substituents), it was concluded that the O-specific polysaccharide had the following structure. ----3)-alpha-L-FucAm-(1----3)-alpha-D-GlcNAc-(1----8)-beta-Sug+  ++-(2---- The O-antigen of S. arizonae O61 is structurally related to that of Pseudomonas aeruginosa O12, thus explaining the known serological cross-reactivity between these micro-organisms.
Vinogradov
E V
EV
N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow.
Shashkov
A S
AS
Knirel
Y A
YA
Kochetkov
N K
NK
Dabrowski
J
J
Grosskurth
H
H
Stanislavsky
E S
ES
Kholodkova
E V
EV
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D007202:Indicators and Reagents; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D019081:O Antigens; D011105:Polyisoprenyl Phosphate Sugars / Q000737:chemistry* / Q000302:isolation & purification; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000302:isolation & purification; D012476:Salmonella arizonae / Q000737:chemistry*; D013045:Species Specificity
D007202:Indicators and Reagents; D019081:O Antigens; C062656:O-specific polysaccharide, Salmonella; D011105:Polyisoprenyl Phosphate Sugars; D011135:Polysaccharides, Bacterial