Stable incorporation of genetic material into the chromosome of Rhizobium meliloti 41: construction of an integrative vector system.
An integrative vector system has been developed from the site-specific recombination elements of temperate phage 16-3. The system can be used for highly efficient stable introduction of genetic material into the chromosome of the symbiotic nitrogen-fixing organism, Rhizobium meliloti 41 (Rm41) at the attB site. Vectors carrying the phage-borne attachment site were constructed, and helper phages providing the site-specific recombination functions in trans were isolated. Other possible applications of the system are discussed.
Hermesz
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E
Attila József University, Department of Genetics, Szeged, Hungary.
Olasz
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F
Dorgai
L
L
Orosz
L
L
D001435:Bacteriophages; D002876:Chromosomes, Bacterial; D003001:Cloning, Molecular; D005822:Genetic Vectors; D010957:Plasmids; D015183:Restriction Mapping; D016962:Sinorhizobium meliloti / Q000235:genetics*