title: 32P-postlabelling analysis of DNA from human tissues.
abstract: DNA extracted from human lung, bladder, liver, pancreas, cervix and breast tissue samples taken at autopsy (37 sample sets) was analysed by the nuclease P1 enhancement modification of the 32P-postlabelling assay for levels of aromatic carcinogen DNA adducts. Results were combined with those from a previous study for statistical analysis of 56 sample sets (32 male+24 female). A strong trend was seen for increased adduct levels in the lung DNA of smokers and a weak association for the bladder DNA of smokers compared to non-smokers. Aromatic adducts were also detected in other tissues.
authors.affiliation: Jack Birch Unit for Environmental Carcinogenesis, Department of Biology, University of York, Heslington, UK.
authors.forename: M N
authors.initials: MN
authors.lastname: Routledge
authors.forename: R C
authors.initials: RC
authors.lastname: Garner
authors.forename: D
authors.initials: D
authors.lastname: Jenkins
authors.forename: J
authors.initials: J
authors.lastname: Cuzick
mesh_terms: D002273:Carcinogens / Q000032:analysis*; D004247:DNA / Q000032:analysis*; D004249:DNA Damage; D005260:Female; D006801:Humans; D008297:Male; D009928:Organ Specificity; D012907:Smoking
chemical_list: D002273:Carcinogens; D004247:DNA