Differentiation of human prostate cancer from benign hypertrophy by in vitro 1H NMR.
In vitro 1H NMR spectra were acquired for perchloric acid extracts of tissue samples of human prostate. Seven patients were diagnosed with prostate cancer, 13 with benign prostatic hypertrophy, and 3 with both conditions. Statistically significant differences between the cancer and benign groups were seen for the metabolite peak area ratios of citrate, creatine, and phosphorylcholine to alanine, and citrate to glutamate. There was no correlation of Gleason grade with any of the ratios measured for the cancer samples. Spectra from different sections of large tumors often yielded substantially different area ratios, confirming the heterogeneous nature of these prostate tumors.
Fowler
A H
AH
Department of Radiology, University of Arkansas for Medical Sciences, Little Rock 72205.
Pappas
A A
AA
Holder
J C
JC
Finkbeiner
A E
AE
Dalrymple
G V
GV
Mullins
M S
MS
Sprigg
J R
JR
Komoroski
R A
RA
D000409:Alanine / Q000378:metabolism; D002951:Citrates / Q000378:metabolism; D003401:Creatine / Q000378:metabolism; D005971:Glutamates / Q000378:metabolism; D018698:Glutamic Acid; D006801:Humans; D066298:In Vitro Techniques; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D010767:Phosphorylcholine / Q000378:metabolism; D011470:Prostatic Hyperplasia / Q000175:diagnosis* / Q000378:metabolism; D011471:Prostatic Neoplasms / Q000175:diagnosis* / Q000378:metabolism
D002951:Citrates; D005971:Glutamates; D010767:Phosphorylcholine; D018698:Glutamic Acid; D003401:Creatine; D000409:Alanine