Sperm-activating peptide type-V (SAP-V), a fifth member of the sperm-activating peptide family, purified from the egg-conditioned media of the heart urchin Brissus agassizii.
1. A novel type of sperm-activating peptide named sperm-activating peptide type-V (SAP-V) was isolated from the egg-conditioned media (egg jelly) of the heart urchin Brissus agassizii and the primary structure of the peptide was determined by fast atom bombardment mass spectrometry as follows: Gly-Cys-Glu-Gly-Leu-Phe-His-Gly-Met-Gly-Asn-Cys. 2. SAP-V and [Met(O)9]SAP-V stimulated the respiration of B. agassizii spermatozoa with half-maximal concentrations of 0.5 and 0.3 nM, respectively. However, half-maximal stimulation of the sperm respiration required 40 nM of S-carboxymethylated SAP-V. 3. SAP-V induced significant increases in the cyclic AMP and cyclic GMP levels in B. agassizii spermatozoa in a concentration-dependent manner. 4. The addition of SAP-V to B. agassizii spermatozoa resulted in a mobility shift of a major sperm protein (mol. wt from 133,000 to 129,000) on sodium dodecyl sulfate-polyacrylamide gels.
Yoshino
K
K
Noto Marine Laboratory, Kanazawa University, Ishikawa, Japan.
Takao
T
T
Shimonishi
Y
Y
Suzuki
N
N
D000595:Amino Acid Sequence; D000818:Animals; D003470:Culture Media; D004527:Egg Proteins / Q000737:chemistry / Q000302:isolation & purification*; D008297:Male; D008715:Methionine / Q000031:analogs & derivatives / Q000032:analysis; D008745:Methylation; D008969:Molecular Sequence Data; D009712:Nucleotides, Cyclic / Q000378:metabolism; D010101:Oxygen Consumption / Q000502:physiology; D010455:Peptides / Q000737:chemistry / Q000302:isolation & purification*; D012617:Sea Urchins / Q000187:drug effects*; D013094:Spermatozoa / Q000187:drug effects
D003470:Culture Media; D004527:Egg Proteins; D009712:Nucleotides, Cyclic; D010455:Peptides; D008715:Methionine; C013111:methionine sulfoxide