title: Peptide fragments of myelin basic protein as substrates of protein kinase C.
abstract: A set of peptides derived from myelin basic protein was synthesized and the kinetics of their phosphorylation by protein kinase C was studied. The replacement or the removal of the N-terminal Gln had no effect on the activity of the parent peptide. The removal of the following Lys or Arg led to a systematic decrease in substrate activity. The modifications in the C-terminal part of the peptide had a weaker influence on the parameters Vmax and KM than those in the N-terminal. The rather regular dependence of the activity of substrates upon their structure does not allow the strict definition of a minimum substrate for protein kinase C.
authors.affiliation: Department of Biochemistry, Uppsala University, Sweden.
authors.forename: M
authors.initials: M
authors.lastname: Eller
authors.forename: J
authors.initials: J
authors.lastname: Järv
authors.forename: R
authors.initials: R
authors.lastname: Toomik
authors.forename: U
authors.initials: U
authors.lastname: Ragnarsson
authors.forename: P
authors.initials: P
authors.lastname: Ekman
authors.forename: L
authors.initials: L
authors.lastname: Engström
mesh_terms: D000595:Amino Acid Sequence; D007700:Kinetics; D008969:Molecular Sequence Data; D004676:Myelin Basic Protein / Q000737:chemistry / Q000378:metabolism*; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism*; D010766:Phosphorylation; D011493:Protein Kinase C / Q000737:chemistry / Q000378:metabolism*; D011761:Pyrrolidonecarboxylic Acid / Q000737:chemistry; D013379:Substrate Specificity
chemical_list: D004676:Myelin Basic Protein; D010446:Peptide Fragments; D011493:Protein Kinase C; D011761:Pyrrolidonecarboxylic Acid