Evaluation of an indirect immunofluorescence assay for confirmation of human immunodeficiency virus type 1 antibody in U.S. blood donor sera.
An indirect immunofluorescence assay (IFA) was evaluated as a confirmatory test for antibody to human immunodeficiency virus type 1 in U.S. blood donor sera previously found to be repeatedly reactive by enzyme immunoassay. IFA results were 100% concordant with a licensed Western blot (immunoblot) for 53 negative and 49 positive samples. Four samples which exhibited antibody to viral proteins from more than one gene, yet were indeterminate by Western blot by the manufacturer's criteria, were also reactive by IFA, whereas 49 additional indeterminate samples were IFA negative.
Sullivan
M T
MT
American Red Cross Holland Laboratory, Rockville, Maryland 20855.
Mucke
H
H
Kadey
S D
SD
Fang
C T
CT
Williams
A E
AE
D015492:AIDS Serodiagnosis / Q000379:methods*; D000956:Antigens, Viral / Q000032:analysis / Q000276:immunology; D001782:Blood Donors; D015153:Blotting, Western; D005069:Evaluation Studies as Topic; D005455:Fluorescent Antibody Technique; D015483:HIV Antibodies / Q000032:analysis*; D015497:HIV-1 / Q000276:immunology* / Q000302:isolation & purification; D006801:Humans
D000956:Antigens, Viral; D015483:HIV Antibodies