Enhanced IgG- and complement-independent phagocytosis of sulfatide-enriched human erythrocytes by human monocytes.
Phagocytosis by adherent human monocytes of human erythrocytes (RBC), sulfatide-enriched by incubation with 10(-12) to 10(-9) M cerebroside sulfate, was enhanced approx. 6-fold. Increased phagocytosis was observed only in RBC opsonized with fresh plasma, and not in non-opsonized or serum-opsonized RBC. Increased phagocytosis was immunoglobulin- and complement independent. Thrombospondin and von Willebrand factor, present in plasma but not in serum, and binding selectively to sulfatides, are likely mediators of the enhanced phagocytosis.
Serra
M V
MV
Istituto di Chimica Biologica e Fisiologia Generale, Università di Sassari, Italy.
Mannu
F
F
Matera
A
A
Turrini
F
F
Arese
P
P
D003165:Complement System Proteins / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004912:Erythrocytes / Q000187:drug effects*; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis / Q000494:pharmacology; D008297:Male; D009000:Monocytes / Q000276:immunology*; D009895:Opsonin Proteins / Q000494:pharmacology; D010587:Phagocytosis / Q000187:drug effects / Q000276:immunology*; D013433:Sulfoglycosphingolipids / Q000494:pharmacology*
D007074:Immunoglobulin G; D009895:Opsonin Proteins; D013433:Sulfoglycosphingolipids; D003165:Complement System Proteins