title: FGF promotes corneal stromal fibroblast motility.
abstract: It is well-known that growth factors accelerate wound healing by stimulating mitosis. Growth factors may also directly stimulate the motility of individual cells. We employed two different experimental methods to determine if fibroblast growth factor (FGF) enhances the individual motility of corneal stromal fibroblasts (independent of mitogenic effects). The effects of FGF on the motility of tissue-cultured rabbit corneal stromal fibroblasts were investigated in a modified Boyden chamber and by the agarose drop motility assay. Both assays showed a significant enhancement of stromal fibroblast motility by FGF at 100 ng/ml. It appears, therefore, that FGF may promote corneal stromal wound healing not only by increased cellular proliferation, but also by increased cellular motility.
authors.affiliation: Department of Ophthalmology, University of Michigan Medical School, Ann Arbor.
authors.forename: R C
authors.initials: RC
authors.lastname: Rao
authors.forename: J
authors.initials: J
authors.lastname: Varani
authors.forename: H K
authors.initials: HK
authors.lastname: Soong
mesh_terms: D000818:Animals; D002452:Cell Count; D002455:Cell Division; D002465:Cell Movement / Q000187:drug effects; D002478:Cells, Cultured; D003319:Corneal Stroma / Q000166:cytology / Q000187:drug effects*; D005346:Fibroblast Growth Factors / Q000494:pharmacology*; D005347:Fibroblasts / Q000187:drug effects; D008297:Male; D011817:Rabbits
chemical_list: D005346:Fibroblast Growth Factors