title: A collagen/gelatin-binding decapeptide derived from bovine propolypeptide of von Willebrand factor.
abstract: Propolypeptide of von Willebrand factor (pp-vWF) binds to type I collagen, and we have reported that a binding domain exists in a 21.5/21-kDa fragment originated from the C-terminal portion [Takagi, J., Fujisawa, T., Sekiya, F., & Saito, Y. (1991) J. Biol. Chem. 266, 5575-5579]. The collagen-binding property of the 21.5/21-kDa fragment was compared with that of the intact pp-vWF. Although pp-v WF preferentially binds to native type I collagen fibrils, the isolated fragment no longer has this specificity and binds well to collagen of other types in the native and heat-denatured states. In order to determine the critical site that mediates this collagen/gelatin binding, several peptides were synthesized based on the primary structure of the 21.5/21-kDa fragment. Among these, a 25-residue peptide strongly inhibited the binding of the 125I-labeled 21.5/21-kDa fragment to collagen. Using this inhibitory effect as an index, the binding site was defined to the sequence as follows: WREPSFCALS. Furthermore, a decapeptide of this sequence bound to collagen and gelatin, indicating that this sequence is responsible for the binding of the 21.5/21-kDa fragment to collagen/gelatin.
authors.affiliation: Department of Biological Sciences, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Japan.
authors.forename: J
authors.initials: J
authors.lastname: Takagi
authors.forename: H
authors.initials: H
authors.lastname: Asai
authors.forename: Y
authors.initials: Y
authors.lastname: Saito
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D001667:Binding, Competitive; D002417:Cattle; D003094:Collagen / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D005780:Gelatin / Q000378:metabolism*; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000378:metabolism*; D011498:Protein Precursors / Q000378:metabolism*; D017385:Sequence Homology; D013379:Substrate Specificity; D014841:von Willebrand Factor / Q000378:metabolism*
chemical_list: D010446:Peptide Fragments; D011498:Protein Precursors; D014841:von Willebrand Factor; D005780:Gelatin; D003094:Collagen