Fusion of the leucine zipper gene HLF to the E2A gene in human acute B-lineage leukemia.
A t(17;19) chromosomal translocation in early B-lineage acute leukemia was shown to result in chimeric transcripts that contain sequences from the E2A basic helix-loop-helix transcription factor gene on chromosome 19, fused to sequences from a previously unidentified gene (HLF) on chromosome 17 that encodes a hepatic leukemia factor. The chimeric protein consisted of the amino-terminal transactivation domain of E2A linked to the carboxyl-terminal basic region-leucine zipper domain of HLF. HLF was normally expressed in liver and kidney, but not in lymphoid cells, and was found to be closely related to the leucine zipper-containing transcription factors DBP (albumin D-box binding protein) and TEF (thyrotroph embryonic factor), which regulate developmental stage-specific gene expression.
Inaba
T
T
Department of Hematology-Oncology, St. Jude Children's Research Hospital, Memphis, TN 38105.
Roberts
W M
WM
Shapiro
L H
LH
Jolly
K W
KW
Raimondi
S C
SC
Smith
S D
SD
Look
A T
AT
D017456:Adenovirus Early Proteins; D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D002051:Burkitt Lymphoma / Q000235:genetics*; D002460:Cell Line; D002886:Chromosomes, Human, Pair 17; D002888:Chromosomes, Human, Pair 19; D003001:Cloning, Molecular; D006801:Humans; D016350:Leucine Zippers / Q000235:genetics*; D008969:Molecular Sequence Data; D005810:Multigene Family; D009838:Oligodeoxyribonucleotides; D009856:Oncogene Proteins, Viral / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D012334:RNA, Neoplasm / Q000235:genetics / Q000302:isolation & purification; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D014157:Transcription Factors / Q000235:genetics*; D014178:Translocation, Genetic
D017456:Adenovirus Early Proteins; D009838:Oligodeoxyribonucleotides; D009856:Oncogene Proteins, Viral; D012334:RNA, Neoplasm; D014157:Transcription Factors