[Clinical significance on expression of the ras gene product P21 in human cervical carcinoma tissues].
Using MAB-P21 as a probe, the expression of gene product was studied by immunohistochemical ABC method, ABC staining of gel electrophoresis and ELISA quantitative analysis. The expression of P21 was detected in 107 cases of uterine cervix tissues, among which 70 were cervical carcinoma, 30 were chronic cervicitis and 7 normal cervix. Experimental results showed that obviously positive expression was noted in the specimens of cervical carcinoma and its positive rate was 72.8% (51/70). It was observed that the expression of P21 varied with grades of cell differentiation in cervical carcinoma. The level of P21 expression in high differentiated type was higher than that of the middle and low differentiated types. On the contrary, there was no detectable ras P21 in the normal cervix tissue and only 2 cases of cervicitis expressed positively (2/30). The results of ABC staining of electrophoresis showed that there was a P21 special staining band in cervical carcinoma tissues. And the contents of P21 protein were higher in cervical carcinoma than in normal cervix and cervicitis (t test, P less than 0.001). The expression of P21 was considerably enhanced in malignant tissue as compared with that in benign lesion.
Huang
G
G
Institute of Cancer Research.
Lu
S
S
Mao
T
T
Song
Y
Y
D000230:Adenocarcinoma / Q000378:metabolism; D000911:Antibodies, Monoclonal; D002294:Carcinoma, Squamous Cell / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D016283:Proto-Oncogene Proteins p21(ras) / Q000032:analysis*; D002583:Uterine Cervical Neoplasms / Q000378:metabolism*
D000911:Antibodies, Monoclonal; C501009:HRAS protein, human; D016283:Proto-Oncogene Proteins p21(ras)