Carbohydrate-binding proteins in bovine kidney have consensus amino acid sequences of annexin family proteins.
Ca(2+)-dependent carbohydrate-binding proteins were purified from bovine kidney extracts. Upon SDS-polyacrylamide gel electrophoresis under nonreducing conditions, the purified fraction gave doublet protein bands corresponding to 33 kDa (p33) and 41 kDa (p41). Under reducing conditions, a single protein band (p33) was observed. p33 and p41 were submitted to proteolytic digestion with endoproteinase Lys-C, the peptides produced were separated by reversed-phase high performance liquid chromatography, and their amino acid sequences were determined by an automated gas-phase protein sequenator. Most of the resulting partial amino acid sequences of these proteins were strikingly homologous to annexin IV, an annexin family protein, i.e. Ca2+/phospholipid-binding proteins, especially in the consensus sequences. In the presence of Ca2+, both proteins bound to vesicles composed of phosphatidylserine and phosphatidylethanolamine, but not phosphatidylcholine. These results indicated that p33 and p41 are members of annexin family proteins.
Kojima
K
K
Department of Chemistry, Faculty of Science, Ochanomizu University, Tokyo, Japan.
Ogawa
H K
HK
Seno
N
N
Yamamoto
K
K
Irimura
T
T
Osawa
T
T
Matsumoto
I
I
D000595:Amino Acid Sequence; D000818:Animals; D017302:Annexins / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D002118:Calcium / Q000378:metabolism; D002417:Cattle; D002846:Chromatography, Affinity; D002848:Chromatography, DEAE-Cellulose; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D007668:Kidney / Q000378:metabolism*; D037102:Lectins / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D008081:Liposomes; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010743:Phospholipids / Q000378:metabolism; D011485:Protein Binding; D017386:Sequence Homology, Amino Acid
D017302:Annexins; D037102:Lectins; D008081:Liposomes; D010743:Phospholipids; D002118:Calcium