Site-specific recombination of the circular 2 microns-like plasmid pKD1 requires integrity of the recombinase gene A and of the partitioning genes B and C.
In the circular plasmid pKD1, which stably replicates in Kluyveromyces lactis, the three open reading frames encode a site-specific recombinase (gene A) and two proteins involved in mitotic stability (genes B and C). A recombination analysis of plasmids in which gene B or C is inactivated reveals that unlike the 2 microns plasmid of Saccharomyces cerevisiae, these genes are also required for the site specificity of plasmid recombination.
Bianchi
M M
MM
Department of Cell and Developmental Biology, University of Rome, La Sapienza, Italy.
D015139:Blotting, Southern; D004254:DNA Nucleotidyltransferases / Q000235:genetics*; D005656:Fungal Proteins / Q000235:genetics; D005800:Genes, Fungal / Q000235:genetics*; D019426:Integrases; D007716:Kluyveromyces / Q000201:enzymology / Q000235:genetics*; D010957:Plasmids / Q000235:genetics*; D045522:Recombinases; D011995:Recombination, Genetic / Q000235:genetics*
D005656:Fungal Proteins; D045522:Recombinases; D004254:DNA Nucleotidyltransferases; D019426:Integrases; C115006:integron integrase IntI1