[A comparative study of the diagnostic value of polyclonal and monoclonal antibodies to human IgM in immunoenzyme diagnostic agents].
Monoclonal antibodies (McAb) to human IgM, capable of recognizing antigenic determinants of different character, have been obtained. Three type-specific McAb have been used in diagnostic systems for the determination of specific IgM antibodies in the sera of patients with hepatitides A and B. The affinity constant and high specificity of McAb have made it possible to change affinity-purified polyclonal antibodies to heavy chains of IgM for the gamma fraction of hybridoma-induced ascitic fluids without decreasing the sensitivity and specificity of test systems. The main advantages of McAb are the standard character of the reagent and reproducibility of its properties.
Tsibinogina
T N
TN
Levitskiĭ
V A
VA
Saulite
V Ia
VIa
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000302:isolation & purification; D000911:Antibodies, Monoclonal / Q000302:isolation & purification; D000915:Antibody Affinity; D000918:Antibody Specificity; D000939:Epitopes / Q000276:immunology; D006508:Hepatitis Antibodies / Q000097:blood; D006510:Hepatitis B Antibodies / Q000097:blood; D006507:Hepatovirus / Q000276:immunology; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007124:Immunoenzyme Techniques; D007075:Immunoglobulin M / Q000097:blood / Q000276:immunology*; D007148:Immunoglobulin mu-Chains / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008258:Waldenstrom Macroglobulinemia / Q000276:immunology
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D000939:Epitopes; D006508:Hepatitis Antibodies; D006510:Hepatitis B Antibodies; D007075:Immunoglobulin M; D007148:Immunoglobulin mu-Chains