title: Localization of the citrate synthase (CS) gene to the p12-p13 bands of chromosome 5 in pigs by in situ hybridization.
abstract: Citrate synthase (CS) is a key enzyme of the Krebs tricarboxylic acid cycle. A 1.4 kb porcine CS cDNA probe was used to chromosomally localize the CS gene in pigs by in situ hybridization. Two in situ hybridization experiments were conducted. Although the first experiment indicated a distinct signal on the 5p12-p13 bands, a secondary signal was observed on the 13q24-q32 bands. Hence, a second in situ hybridization experiment was conducted at higher stringency. The results demonstrated a consistent signal on the 5p12-p13 bands, and the signal on chromosome 13 was scattered with no prominent secondary peak. The CS gene was therefore assigned to the p12-p13 bands of chromosome 5 in pigs.
authors.affiliation: Department of Animal Breeding and Genetics, Swedish University of Agricultural Sciences, Uppsala.
authors.forename: R
authors.initials: R
authors.lastname: Chaudhary
authors.forename: B P
authors.initials: BP
authors.lastname: Chowdhary
authors.forename: I
authors.initials: I
authors.lastname: Harbitz
authors.forename: I
authors.initials: I
authors.lastname: Gustavsson
authors.forename: C T
authors.initials: CT
authors.lastname: Evans
mesh_terms: D000818:Animals; D002871:Chromosome Banding; D002874:Chromosome Mapping / Q000379:methods*; D002950:Citrate (si)-Synthase / Q000235:genetics*; D017403:In Situ Hybridization; D013552:Swine
chemical_list: D002950:Citrate (si)-Synthase