title: Coupled-column high-performance liquid chromatographic method for the determination of 1-hydroxypyrene in urine of subjects exposed to polycyclic aromatic hydrocarbons.
abstract: A coupled-column high-performance liquid chromatographic system for integrated, on-line sample processing and the determination of free and conjugated 1-hydroxypyrene in urine has been developed. The method is based on a "tailor-made" copper phthalocyanine-modified porous-glass precolumn packing material, which allows a direct and repeated injection of urine samples and a selective enrichment of trace amounts of particular components. The fully automated method has a low detection limit (0.01 pmol), a quantitative and matrix-independent recovery and a highly reliability, as shown by an interlaboratory comparison of methods.
authors.affiliation: Institut für Klinische Chemie, Klinikum Grosshadern, Universität München, Germany.
authors.forename: K S
authors.initials: KS
authors.lastname: Boos
authors.forename: J
authors.initials: J
authors.lastname: Lintelmann
authors.forename: A
authors.initials: A
authors.lastname: Kettrup
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D015394:Molecular Structure; D016273:Occupational Exposure; D011083:Polycyclic Compounds / Q000032:analysis* / Q000378:metabolism; D011721:Pyrenes / Q000032:analysis* / Q000737:chemistry; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence; D016482:Urinalysis / Q000379:methods
chemical_list: D011083:Polycyclic Compounds; D011721:Pyrenes; C033146:1-hydroxypyrene