Evidence that umbilical cord blood contains a higher frequency of HLA class II-specific alloreactive T cells than adult peripheral blood. A limiting dilution analysis.
Umbilical cord blood has been used to effect hematological reconstitution and there are sufficient stem cells available in the cord blood obtainable from a single placenta to reconstitute an adult patient. Umbilical cord blood might therefore, have widespread potential use in the field of bone marrow transplantation. We compared alloreactivity of paired samples of adult and cord peripheral blood mononuclear cells, by measuring frequencies of both alloreactive T helper cells and cytotoxic T cell precursors (CTLp), using limiting dilution analysis. In addition we compared the phenotype of adult and neonatal PBMC, using monoclonal antibody staining. Cord PBMC in general showed higher frequencies of alloreactive Th than adult PBMC, with statistically significant differences in 6 out of 10 experiments. There was no statistically significant difference between adult and cord CTLp frequencies. Adult and cord PBMC surface phenotype was similar, except that cord blood contained fewer lymphocyte function-associated-3 (LFA-3) positive (memory) cells.
Deacock
S J
SJ
Department of Immunology, Royal Postgraduate Medical School, London, England.
Schwarer
A P
AP
Bridge
J
J
Batchelor
J R
JR
Goldman
J M
JM
Lechler
R I
RI
D000328:Adult; D000954:Antigens, Surface / Q000032:analysis; D018968:CD58 Antigens; D005312:Fetal Blood / Q000276:immunology*; D000949:Histocompatibility Antigens Class II / Q000097:blood*; D006801:Humans; D007156:Immunologic Memory; D007518:Isoantibodies; D008562:Membrane Glycoproteins / Q000032:analysis; D013234:Stem Cells / Q000166:cytology; D013601:T-Lymphocytes / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000166:cytology; D006377:T-Lymphocytes, Helper-Inducer / Q000166:cytology
D000954:Antigens, Surface; D018968:CD58 Antigens; D000949:Histocompatibility Antigens Class II; D007518:Isoantibodies; D008562:Membrane Glycoproteins