title: Diverse locations of amino acids in HLA-DR beta chains involved in polymorphic antibody binding epitopes on DR(alpha, beta 1*0101), DR(alpha, beta 1*1101), and DR(alpha,beta 3*0202) molecules.
abstract: In a previous study, we used transfectants expressing hybrid HLA-DR(beta 1*0403)/DR(beta 1*0701) chains to map sequences involved in polymorphic antibody binding epitopes on DR(alpha, beta 1*0403) or DR(alpha, beta 1*0701) molecules. Amino acids 1-40 of the beta 1 domain were found to make the major contributions to most of the antibody binding epitopes studied. To begin to localize sequences that contribute to polymorphic antibody epitopes on DR(alpha,beta 1*0101), DR(alpha,beta 1*1101) and DR(alpha,beta 3*0202) molecules, we used indirect immunofluorescence and flow cytometry to assess the binding of mAb to transfectants expressing hybrid DR(beta 1*0101)/DR(beta 1*1101) or DR(beta 1*1101)/DR(beta 3*0202) chains that divide the DR beta chain into three segments: amino acids 1-40, 41-97, and the beta 2 domain. The results indicate that amino acids 41-97 of the beta 1 domain on DR(beta 1*0101), DR(beta 1*1101), or DR(beta 3*0202) are critical in most of the epitopes, including those recognized by human antibodies MP4 and MP12, and mouse mAb GS88.2, I-LR1, 21r5, and 7.3.19.1, whereas amino acids 1-40 of DR(beta 1*1101) are critical in the epitope recognized by the MCS-7 mAb, and both segments 1-40 and 41-97 of DR(beta 1*1101) are important in the epitopes recognized by the I-LR2 and UL-52 mAbs. Based on these data and comparison of DR beta allelic protein sequences, the residues that may play critical roles in these antibody binding epitopes are predicted.
authors.affiliation: Department of Veterans Affairs Medical Center, Iowa City, Iowa.
authors.forename: X T
authors.initials: XT
authors.lastname: Fu
authors.forename: E
authors.initials: E
authors.lastname: Klohe
authors.forename: C
authors.initials: C
authors.lastname: Alber
authors.forename: W Y
authors.initials: WY
authors.lastname: Yu
authors.forename: G B
authors.initials: GB
authors.lastname: Ferrara
authors.forename: M P
authors.initials: MP
authors.lastname: Pistillo
authors.forename: M
authors.initials: M
authors.lastname: Ballas
authors.forename: R W
authors.initials: RW
authors.lastname: Karr
mesh_terms: D000596:Amino Acids / Q000032:analysis*; D000939:Epitopes / Q000032:analysis*; D006684:HLA-DR Antigens / Q000276:immunology*; D006801:Humans; D013329:Structure-Activity Relationship
chemical_list: D000596:Amino Acids; D000939:Epitopes; D006684:HLA-DR Antigens