title: Membrane instability in respiring mitochondria: role of phosphate.
abstract: Metabolically-induced (spontaneous) high amplitude swelling of mitochondria has been shown to be due to a serial disruption of the mitochondrial membranes [D. Sambasivarao & V. Sitaramam (1985), Biochim Biophys Acta, 806, 195-209]. Phosphate- and arsenate-induced swelling was investigated in mitochondria to evaluate the role of phosphate transport in the instability created in the mitochondrial membranes. Phosphate-induced swelling in respiring mitochondria was similar to spontaneous swelling. Both represent essentially colloidal swelling due to the variable porosity induced in the inner membrane to polyols by respiration. Swelling of non-respiring mitochondria at high ammonium phosphate concentrations was, on the other hand, primarily due to high permeability to phosphate. This membrane instability created by phosphate transport in the surrounding lipid involves neither the endogenous nor the exogenous Ca2+.
authors.affiliation: Department of Zoology, University of Pune, Ganeshkhind.
authors.forename: V
authors.initials: V
authors.lastname: Sitaramam
authors.forename: N M
authors.initials: NM
authors.lastname: Rao
mesh_terms: D000818:Animals; D007425:Intracellular Membranes / Q000187:drug effects / Q000502:physiology*; D008353:Mannitol / Q000494:pharmacology; D008930:Mitochondria, Liver / Q000502:physiology*; D008933:Mitochondrial Swelling / Q000187:drug effects; D010101:Oxygen Consumption; D010710:Phosphates / Q000378:metabolism / Q000494:pharmacology*; D051381:Rats; D013395:Sucrose / Q000494:pharmacology
chemical_list: D010710:Phosphates; D008353:Mannitol; D013395:Sucrose