Two chicken monoclonal antibodies specific for heterophil Hanganutziu-Deicher antigens.
Two chicken monoclonal antibodies (MAbs), HU/Ch2-7 and HU/Ch6-1, against heterophil Hanganutziu-Deicher (HD) antigens with N-glycolylneuraminic acid (NeuGc) at a terminal carbohydrate were established by cell fusions using chicken B cell lines lacking thymidine kinase and spleen cells from chickens immunized with II3NeuGc alpha-LacCer (HD3). The reactivities of these MAbs against several gangliosides including NeuGc-containing glycosphingolipids were examined by a thin-layer chromatography/immunostaining method. MAb HU/Ch2-7 (IgG) reacted strongly with HD3 and IV3NeuGc alpha-nLc4Cer (HD5) and weakly with VI3NeuGc alpha-nLc6Cer (HD7) and 4-O-acetyl-HD3. HU/Ch6-1 (IgG) reacted with HD3 and HD5, but did not react with the other HD antigens. The reactivities of these MAbs against HD antigen were greatly reduced by pre-treatment of the antigen with neuraminidase. These MAbs did not react with N-acetylneuraminic acid-containing gangliosides (GM1 and GM3). These results indicate that these two chicken MAbs are directed toward the antigenic epitope containing the NeuGc.
Asaoka
H
H
Department of Microbiology and Hygiene, Faculty of Applied Biological Science, Hiroshima University, Japan.
Nishinaka
S
S
Wakamiya
N
N
Matsuda
H
H
Murata
M
M
D000818:Animals; D000910:Antibodies, Heterophile / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D015478:Antigens, Heterophile / Q000187:drug effects / Q000276:immunology*; D002240:Carbohydrate Sequence; D002645:Chickens / Q000276:immunology*; D000939:Epitopes / Q000276:immunology; D006825:Hybridomas / Q000276:immunology; D008969:Molecular Sequence Data; D009438:Neuraminic Acids / Q000276:immunology; D009439:Neuraminidase / Q000494:pharmacology
D000910:Antibodies, Heterophile; D000911:Antibodies, Monoclonal; D015478:Antigens, Heterophile; D000939:Epitopes; D009438:Neuraminic Acids; C032592:N-glycolylneuraminic acid; D009439:Neuraminidase