title: Comparison of hepatitis C virus markers in patients with NANB hepatitis.
abstract: 10 different HCV-specific assays and RT-PCR of the 5' untranslated region of HCV RNA were used to analyze sixty-four patients with chronic NANB liver disease. Po, CP-9 and C22 antigens are located in the putative core; C33c in the putative NS3; C100-3 in the putative NS3/4; KCL in the putative NS4/5 and C825 is located in the putative NS5. GOR protein is not part of the HCV genome, but antibodies to it appear to be present in response to a hepatitis C infection. Positive rates were 91% for Po, 89% for CP-9, 94% for C22, 97% for C33c, 88% for C100-3 (Ortho, EIA), 86% for C100-3 (Abbott, EIA), 84% for C100-3 (Ohtsuka, RIA), 88% for KCL, 59% for C825, 58% for GOR, and 83% for RT-PCR. There were 8 cases which were negative by all anti-C100 tests. 7 of these cases were positive by other anti-HCV markers and/or PCR suggesting the need for improved blood screening assays. There is a variation in the relative reactivity for different markers with different samples. Of the tests employed, anti C33c shows the highest positivity rate.
authors.affiliation: Department of Clinical Research, Nagasaki Chuo National Hospital, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Yatsuhashi
authors.forename: O
authors.initials: O
authors.lastname: Inoue
authors.forename: M
authors.initials: M
authors.lastname: Koga
authors.forename: S
authors.initials: S
authors.lastname: Nagataki
authors.forename: K
authors.initials: K
authors.lastname: Mizuno
authors.forename: J
authors.initials: J
authors.lastname: Kolberg
authors.forename: E
authors.initials: E
authors.lastname: Beall
authors.forename: T A
authors.initials: TA
authors.lastname: Cha
authors.forename: B
authors.initials: B
authors.lastname: Irvine
authors.forename: G
authors.initials: G
authors.lastname: Kuo
mesh_terms: D000328:Adult; D000368:Aged; D000956:Antigens, Viral / Q000097:blood / Q000276:immunology; D015415:Biomarkers / Q000097:blood*; D005260:Female; D016174:Hepacivirus / Q000276:immunology; D006508:Hepatitis Antibodies / Q000097:blood; D006526:Hepatitis C / Q000097:blood* / Q000276:immunology; D018937:Hepatitis C Antibodies; D018936:Hepatitis C Antigens; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity
chemical_list: D000956:Antigens, Viral; D015415:Biomarkers; D006508:Hepatitis Antibodies; D018937:Hepatitis C Antibodies; D018936:Hepatitis C Antigens