The PSI-E subunit of photosystem I binds ferredoxin:NADP+ oxidoreductase.
A photosystem I complex containing the polypeptides PSI-A to PSI-L, light-harvesting complex I and ferredoxin:NADP+ oxidoreductase has been isolated from barley using the non-ionic detergent n-decyl-beta-D-maltopyranoside. The ratio between bound ferredoxin:NADP+ oxidoreductase and P700 is 0.4 +/- 0.2. The complex is highly active in catalyzing light-induced transfer of electrons from plastocyanin to NADP+ at rates of 280 +/- 150 and 1800 +/- 800 mumol NADPH/(mg chl.h), without and in the presence of saturating amounts of exogenously added ferredoxin:NADP+ oxidoreductase, respectively. Endogenously bound ferredoxin:NADP+ oxidoreductase interacts with the PSI-E subunit as demonstrated by cross-linking experiments using two different types of cross-linkers and identification of the products by Western blotting and the use of monospecific antibodies.
Andersen
B
B
Department of Plant Biology, Royal Veterinary and Agricultural University, Copenhagen, Denmark.
Scheller
H V
HV
Møller
B L
BL
D003432:Cross-Linking Reagents; D004579:Electron Transport; D005022:Ethyldimethylaminopropyl Carbodiimide / Q000494:pharmacology; D005287:Ferredoxin-NADP Reductase / Q000378:metabolism*; D001467:Hordeum / Q000737:chemistry; D008027:Light; D008970:Molecular Weight; D045322:Photosynthetic Reaction Center Complex Proteins / Q000302:isolation & purification / Q000378:metabolism*; D045331:Photosystem I Protein Complex; D010970:Plastocyanin / Q000378:metabolism; D013388:Succinimides / Q000494:pharmacology
D003432:Cross-Linking Reagents; D045322:Photosynthetic Reaction Center Complex Proteins; D045331:Photosystem I Protein Complex; D013388:Succinimides; C035759:3,3'-dithiobis(sulfosuccinimidyl propionate); D010970:Plastocyanin; D005287:Ferredoxin-NADP Reductase; D005022:Ethyldimethylaminopropyl Carbodiimide