Microcarrier culture of fish cells and viruses in cell culture bioreactor.
This article deals with the culture of grass carp (Ctenopharyngodon idellus) lip and embryo cells on Cytodex 3 and GT-2 microcarriers in a 1.5-L cell culture bioreactor to propagate grass carp hemorrhage virus. The cells and viruses were successfully cultivated at 26 degrees C, pH 7.0, and dissolved oxygen 40% of air saturation. The cell density achieved was as high as 7.4 x 10(6) cells/mL, and the virus titre reached 6.75 log LD50/0.5 mL from an initial 3.00 log LD50/0.5 mL. The results present broad prospects for fish virus vaccine production.
Chen
Z
Z
Research Institute of Biochemical Engineering, East China University of Chemical Technology, Shanghai.
Chen
Y
Y
Shi
Y
Y
Yie
X
X
Yang
G
G
D000818:Animals; D001709:Biotechnology / Q000295:instrumentation*; D002347:Carps / Q000033:anatomy & histology* / Q000378:metabolism / Q000382:microbiology*; D002455:Cell Division; D002460:Cell Line; D003584:Cytological Techniques / Q000295:instrumentation; D005393:Fish Diseases / Q000382:microbiology / Q000517:prevention & control; D005947:Glucose / Q000378:metabolism; D007700:Kinetics; D014765:Viral Vaccines / Q000302:isolation & purification; D014776:Virus Cultivation / Q000295:instrumentation; D014780:Viruses / Q000254:growth & development
D014765:Viral Vaccines; D005947:Glucose