title: Expression of collagenase and potential transcriptional factors c-fos and egr-1 in periodontal gingival fibroblasts.
abstract: In view of the important role of fibroblast-type collagenase in the pathogenesis of periodontal disease (PD), we investigated the expression of this metalloproteinase in primary cultures of non-stimulated fibroblasts dissected from gingival tissues of patients with generalized moderate and localized severe chronic adult PD. Enhanced hybridization signals for collagenase RNA were observed in 8/8 PD-cases when compared with equivalent RNA amounts extracted from normal fibroblasts. Since both the proto-oncogene c-fos and the "early growth response" gene egr-1 might be involved in the transcriptional regulation of the collagenase gene expression in vivo, we also compared the relative expression of both potential transcriptional factors with collagenase RNA in the same fibroblast cytoplasmic extracts. Hybridization signals indicated elevated RNA amounts for c-fos in 8/8 PD-cases and for egr-1 in 7/8 PD-cases when compared with the cells from non-inflamed tissue. In periodontitis gingival tissue specimens, immunolocalization of collagenase could be confirmed in fibroblasts, macrophages and epithelial cells in situ. Collagenase label was not widely distributed within the tissues, but concentrated at the interface between epithelium and connective tissue. The data provide the first evidence that gingival fibroblasts producing elevated levels of collagenase RNA amounts express also c-fos and egr-1 indicating a crucial role for both genes in cellular proliferation and collagenase expression in gingival and periodontal tissue destruction in vivo.
authors.affiliation: Research Center of Oral Biology, School of Dentistry, University of Albama, Birmingham.
authors.forename: A
authors.initials: A
authors.lastname: Trabandt
authors.forename: R E
authors.initials: RE
authors.lastname: Gay
authors.forename: V P
authors.initials: VP
authors.lastname: Sukhatme
authors.forename: S
authors.initials: S
authors.lastname: Gay
mesh_terms: D016301:Alveolar Bone Loss / Q000235:genetics / Q000378:metabolism; D017364:Collagenases / Q000235:genetics* / Q000378:metabolism; D015342:DNA Probes; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D051766:Early Growth Response Protein 1; D005347:Fibroblasts / Q000378:metabolism*; D015870:Gene Expression; D016762:Genes, fos / Q000235:genetics*; D005881:Gingiva / Q000378:metabolism / Q000473:pathology*; D005885:Gingival Hyperplasia / Q000235:genetics / Q000378:metabolism; D006801:Humans; D017874:Immediate-Early Proteins; D015151:Immunoblotting; D007124:Immunoenzyme Techniques; D008875:Middle Aged; D009693:Nucleic Acid Hybridization; D010518:Periodontitis / Q000235:genetics* / Q000378:metabolism; D000090063:Proto-Oncogene Mas; D012313:RNA / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D016335:Zinc Fingers / Q000235:genetics*
chemical_list: D015342:DNA Probes; D004268:DNA-Binding Proteins; C497325:EGR1 protein, human; D051766:Early Growth Response Protein 1; D017874:Immediate-Early Proteins; C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D014157:Transcription Factors; D012313:RNA; D017364:Collagenases