[Light-optical and ultrastructural immunohistochemistry of antigen H-4, a common marker of epithelial cells].
Epitope localization reacting with mice monoclonal antibodies (Mabs) H 4 was investigated using the specimen of epithelium of skin, human uterine cervix as well as in the culture of epithelium cells of guinea-pig duct deferent. Mice monoclonal antibodies against antigen H 4 obtained by the hybridoma method after immunization of mice with rat colon epithelium cytoskeletal fractions were used. Mabs H 4 were shown to react with antigen of intermediate filaments of all studied normal epithelial, cancer cells as well as culture epithelial cells. Mabs H 4 are supposed to be used as a unique immunohistochemical marker of epithelium cells under normal and malignant growth conditions.
Petrov
S V
SV
Raĭkhlin
N T
NT
Nizamov
R S
RS
D000328:Adult; D000818:Animals; D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm / Q000032:analysis*; D002478:Cells, Cultured; D003599:Cytoskeleton / Q000276:immunology; D004848:Epithelium / Q000276:immunology / Q000648:ultrastructure; D000939:Epitopes / Q000276:immunology; D005260:Female; D006168:Guinea Pigs; D006801:Humans; D007114:Immunization; D007150:Immunohistochemistry; D051379:Mice; D051381:Rats; D012867:Skin / Q000276:immunology / Q000648:ultrastructure; D014407:Tumor Cells, Cultured / Q000276:immunology
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D000939:Epitopes