Purification of listeriolysin O and development of an immunoassay for diagnosis of listeric infections in sheep.
A protein of 58,000-Da molecular mass was purified from the supernatant fluid of a dialysis sac culture of Listeria monocytogenes by cation-exchange chromatography. The purified protein, homogeneous by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and possessing the characteristics of listeriolysin O (LLO), was used to develop an indirect enzyme-linked immunosorbent assay. Anti-LLO antibodies were shown to be consistently produced in sheep after experimental challenge with L. monocytogenes serovar 4b. The assay also successfully detected and measured specific anti-LLO antibodies in the sera of silage-fed sheep among which listeric enteritis and abortions had occurred.
Low
J C
JC
Edinburgh Veterinary Investigation Centre, Scottish Agricultural College, Penicuik, Midlothian.
Davies
R C
RC
Donachie
W
W
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D001426:Bacterial Proteins / Q000276:immunology / Q000302:isolation & purification*; D001427:Bacterial Toxins; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D006360:Heat-Shock Proteins / Q000276:immunology / Q000302:isolation & purification*; D006460:Hemolysin Proteins / Q000276:immunology / Q000302:isolation & purification*; D008088:Listeriosis / Q000175:diagnosis / Q000662:veterinary*; D012698:Serologic Tests; D012756:Sheep; D012757:Sheep Diseases / Q000175:diagnosis*
D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D006360:Heat-Shock Proteins; D006460:Hemolysin Proteins; C038705:hlyA protein, Listeria monocytogenes