Active site studies of human immunodeficiency virus reverse transcriptase.
The active site of human immunodeficiency virus reverse transcriptase (HIV1-RT) was probed using three group-specific reagents: phenylglyoxal (PG), N-ethylmaleimide (NEM), and pyridoxal 5'-phosphate (PLP). The inactivation of HIV1-RT by arginine-specific PG was found to be completely protected against by adding primer-template. The potential active site arginine was localized to position 277 in the primary structure, suggesting that the polymerase domain of the enzyme should be considered as extending at least this far from the N terminus. The sulfhydryl-modifying reagent NEM completely inhibits NY5-HIV1-RT, which contains a cysteine at position 162, and such inhibition is protected against by primer-template. However, it does not strongly inhibit LAV-HIV1-RT, in which C162 is replaced by S162, indicating that while C162 may be at or near the active site or interact allosterically with primer-template, it is not essential for activity. The lysine-specific reagent PLP was found to be a noncompetitive inhibitor with respect to both primer-template [poly(rA).oligo(dT)] and dTTP. The latter result differentiates HIV1-RT from other RTs, for which PLP has been shown to be a competitive inhibitor with respect to dTTP.
Mitchell
L L
LL
Department of Chemistry, University of Pennsylvania, Philadelphia 19104-6323.
Cooperman
B S
BS
D000595:Amino Acid Sequence; D001120:Arginine; D001665:Binding Sites; D002851:Chromatography, High Pressure Liquid; D005033:Ethylmaleimide / Q000378:metabolism* / Q000494:pharmacology; D006678:HIV / Q000201:enzymology*; D054303:HIV Reverse Transcriptase; D015497:HIV-1 / Q000201:enzymology*; D007700:Kinetics; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000302:isolation & purification; D010658:Phenylglyoxal / Q000378:metabolism* / Q000494:pharmacology; D011732:Pyridoxal Phosphate / Q000378:metabolism* / Q000494:pharmacology; D012194:RNA-Directed DNA Polymerase / Q000737:chemistry / Q000378:metabolism*; D011994:Recombinant Proteins / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000378:metabolism; D018894:Reverse Transcriptase Inhibitors; D016914:Ribonuclease H / Q000378:metabolism; D012689:Sequence Homology, Nucleic Acid; D014357:Trypsin
D010446:Peptide Fragments; D011994:Recombinant Proteins; D018894:Reverse Transcriptase Inhibitors; D011732:Pyridoxal Phosphate; D001120:Arginine; D054303:HIV Reverse Transcriptase; D012194:RNA-Directed DNA Polymerase; D016914:Ribonuclease H; D014357:Trypsin; D010658:Phenylglyoxal; D005033:Ethylmaleimide