Histological and immunohistochemical investigations of hydroxyethyl-starch deposits in rat tissues.
Tissue storage of hydroxyethyl starch (HES), a widely used artificial colloid, has been reported. In order to clarify whether storage of HES can be detected in tissues by immunohistochemical methods, use was made of a polyclonal rabbit anti-HES antiserum. Thirteen days after a single intravenous injection of HES rats were sacrificed and liver, spleen, lymph node, lung, kidney and skin were removed. On paraffin sections in all organs the anti-HES antiserum stained mainly cells which could be attributed to the mononuclear phagocyte system, as confirmed by the use of the antimacrophage monoclonal antibody ED1. The use of a polyclonal anti-HES antiserum may allow analysis of long-term storage and possible side effects in various tissues of man.
Parth
E
E
Institute of General and Experimental Pathology, University of Vienna, Austria.
Jurecka
W
W
Szépfalusi
Z
Z
Schimetta
W
W
Gebhart
W
W
Scheiner
O
O
Kraft
D
D
D000818:Animals; D006895:Hydroxyethyl Starch Derivatives / Q000032:analysis*; D007150:Immunohistochemistry; D008099:Liver / Q000737:chemistry*; D008198:Lymph Nodes / Q000737:chemistry*; D008297:Male; D051381:Rats; D011917:Rats, Inbred Lew; D013154:Spleen / Q000737:chemistry*
D006895:Hydroxyethyl Starch Derivatives