Initial hepatic metabolic function in canine liver and pancreas cluster transplantation.
In this study, initial hepatic metabolic function was evaluated by determining the arterial ketone body ratio (AKBR) and plasma amino acid concentrations in an experimental orthotopic combined hepatopancreatic transplantation (OHPT), and comparing the same values in orthotopic liver transplantation (OLT). In OHPT, AKBR decreased in the anhepatic phase and recovered to the preoperative value just 1 h after reperfusion. On the other hand, in OLT, the recovery of AKBR took 3 h after reperfusion with a significant difference compared to OHPT (P less than 0.05). Plasma amino acid levels, especially alanine and total free plasma amino acids increased in the anhepatic phase and recovered within 1 h of reperfusion in OHPT. However, they did not recover until 3 h after reperfusion in OLT. This rapid recovery of hepatic metabolic function in OHPT should be attributed to the order of reperfusion in which the reconstruction of arterial blood flow precedes that of portal blood flow. This model is useful for assessing the best way by which the grafted liver can for assessing the best way by which the grafted liver can control the timing, order, rate, and volume of blood that should be released.
Hisanaga
M
M
First Department of Surgery, Nara Medical University, Japan.
Nakajima
Y
Y
Segawa
M
M
Matsumoto
M
M
Fukuoka
T
T
Yabuuchi
H
H
Kido
K
K
Horikawa
M
M
Taki
J
J
Yoshimura
A
A
D000596:Amino Acids / Q000097:blood; D000818:Animals; D004195:Disease Models, Animal; D004285:Dogs; D007657:Ketone Bodies / Q000097:blood; D008099:Liver / Q000378:metabolism*; D016031:Liver Transplantation / Q000379:methods; D010179:Pancreas / Q000378:metabolism*; D016035:Pancreas Transplantation / Q000379:methods; D011184:Postoperative Period; D015424:Reperfusion
D000596:Amino Acids; D007657:Ketone Bodies