Mallory stain may indicate differential rates of RNA synthesis: II. Comparative observations in vertebrate nuclei.
The differential staining of nuclei by the use of the Mallory trichrome method was investigated in a variety of tissues of representative vertebrates. By this method nuclei stained orange or blue; erythrocyte nuclei stained red. Since the higher affinity for aniline blue is due to an increased RNA synthesis, it was possible to reveal not only the changing metabolic status of a cell type, as shown for instance in the liver parenchyma and other glandular tissues, and nervous tissue, but also in different cell populations in the same tissue, such as the spleen.
Chieffi Baccari
G
G
Istituto di Anatomia Umana Normale, Università Federico II, Napoli, Italy.
Marmorino
C
C
Minucci
S
S
Di Matteo
L
L
d'Istria
M
M
D000663:Amphibians; D000818:Animals; D001345:Autoradiography; D001717:Birds; D002467:Cell Nucleus / Q000378:metabolism* / Q000648:ultrastructure; D004912:Erythrocytes / Q000648:ultrastructure; D005399:Fishes; D006651:Histocytochemistry; D051379:Mice; D012313:RNA / Q000096:biosynthesis*; D011817:Rabbits; D051381:Rats; D012104:Reptiles; D016707:Tissue Fixation
D012313:RNA