title: The effect of divalent metal ions on the thermostability of C3.
abstract: Thermostability studies on human complement component C3 showed that saturation of the electronegative sites in the C3 molecule with divalent cations offered some protection against heat denaturation, a finding not previously reported in the literature. To inactive C3 in about 30 minutes, the required temperature was 61 degrees C in the presence of excess Ca2+ ions, 59.5 degrees C in the presence of excess of Mg2+ ions, but only 54 degrees C in the absence of these ionized metal ions.
authors.affiliation: Department of Health Sciences, Hong Kong Polytechnic, Hung Hom, Kowloon.
authors.forename: S P
authors.initials: SP
authors.lastname: Yip
authors.forename: W H
authors.initials: WH
authors.lastname: Lewis
mesh_terms: D002118:Calcium / Q000494:pharmacology; D002413:Cations, Divalent / Q000494:pharmacology*; D003176:Complement C3 / Q000737:chemistry / Q000187:drug effects*; D004492:Edetic Acid / Q000494:pharmacology; D006358:Hot Temperature; D006801:Humans; D008274:Magnesium / Q000494:pharmacology; D011489:Protein Denaturation / Q000187:drug effects
chemical_list: D002413:Cations, Divalent; D003176:Complement C3; D004492:Edetic Acid; D008274:Magnesium; D002118:Calcium