Platelets and vitronectin: immunocytochemical localization and platelet interaction with exogenously added vitronectin.
Vitronectin stores were localized in human platelets by immunofluorescence, confocal laser microscopy, and immunoelectron microscopy. In ultrathin cryosections, the immunogold labelling was mainly observed in the alpha-granules, although occasional immunolabelling of the platelet surface was present. The interaction between thrombin-stimulated platelets and exogenously added vitronectin was also explored. This vitronectin had been purified by heparin-affinity chromatography. Binding of vitronectin to thrombin-stimulated platelets was demonstrated by flow cytometry. The immunoelectron microscopical studies revealed that this binding was mainly restricted to clumps of released alpha-granular material on the platelet surface. The possible significance of the interaction between exogenous vitronectin and released platelet proteins is discussed.
Røger
M
M
Institute of Pathology, Rikshospitalet, Oslo, Norway.
Halstensen
T S
TS
Høgåsen
K
K
Mollnes
T E
TE
Solum
N O
NO
Hovig
T
T
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000276:immunology; D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D003594:Cytoplasmic Granules / Q000737:chemistry; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D006023:Glycoproteins / Q000097:blood*; D006801:Humans; D007150:Immunohistochemistry; D016253:Microscopy, Immunoelectron; D008969:Molecular Sequence Data; D010980:Platelet Membrane Glycoproteins / Q000276:immunology; D011485:Protein Binding; D013917:Thrombin / Q000494:pharmacology; D019096:Vitronectin
D000911:Antibodies, Monoclonal; D006023:Glycoproteins; D010980:Platelet Membrane Glycoproteins; D019096:Vitronectin; D013917:Thrombin