title: Preparation of benzoyl dextran and its use in aqueous two-phase systems.
abstract: The graft modification of dextran with benzoyl groups has been studied. The factors that affect the degree of substitution of benzoyl dextran were investigated. Phase diagrams for aqueous two-phase systems composed of polyethylene glycol/benzoyl dextran and dextran/benzoyl dextran have been determined. Phase separation was also obtained in aqueous solution of two benzoyl dextran polymers with different degrees of substitution. A four-phase system was obtained with a mixture of polyethylene glycol, dextran and two kinds of benzoyl dextrans. The partitioning of methylene blue and a Procion yellow HE-3G dextran derivative were studied in polyethylene glycol/benzoyl dextran and dextran/benzoyl dextran two-phase systems and in systems of two benzoyl dextrans differing in degree of substitution. The proteins bovine serum albumin and glucose-6-phosphate dehydrogenase were partitioned in polyethylene glycol/benzoyl dextran aqueous two-phase systems and the effect of the degree of substitution of benzoyl dextran was studied. Chlorella pyrenoidosa, thylakoid membrane vesicles, plasma membrane vesicles and chloroplasts were partitioned in polyethylene glycol/benzoyl dextran and dextran/benzoyl dextran two-phase systems, and in a polyethylene glycol/dextran/benzoyl dextran four-phase system.
authors.affiliation: Department of Biochemistry, University of Lund, Sweden.
authors.forename: M
authors.initials: M
authors.lastname: Lu
authors.forename: F
authors.initials: F
authors.lastname: Tjerneld
authors.forename: G
authors.initials: G
authors.lastname: Johansson
authors.forename: P A
authors.initials: PA
authors.lastname: Albertsson
mesh_terms: D002462:Cell Membrane; D002736:Chloroplasts; D003911:Dextrans; D008751:Methylene Blue; D011092:Polyethylene Glycols; D011506:Proteins / Q000302:isolation & purification*; D012995:Solubility
chemical_list: D003911:Dextrans; D011506:Proteins; D011092:Polyethylene Glycols; D008751:Methylene Blue