Changes in membrane permeability during Semliki Forest virus induced cell fusion.
The infection of Aedes albopictus cells by Semliki Forest virus (SFV) is a non lytic event. Exposure of infected cells to mildly acidic pH (less than 6.2) leads to syncytium formation. This polykaryon formation is accompanied by an influx of protons into the cells (Kempf et al. Biosci. Rep. 7, 761-769, 1987). We have further investigated this permeability change using various fluorescent or radiolabeled compounds. A significant, pH dependent increase of the membrane permeability to low molecular weight compounds (M(r) less than 1000) was observed when infected cells were exposed to a pH less than 6.2. The pH dependence of the permeability change was very similar to the pH dependence of cell-cell fusion. The permeability change was sensitive to divalent cations, protons and anionic antiviral drugs such as trypan blue. The nature of this virus induced, pH dependent permeability change is discussed.
Lanzrein
M
M
Institute of Biochemistry, University of Bern, Switzerland.
Käsermann
N
N
Kempf
C
C
D000330:Aedes; D000818:Animals; D002413:Cations, Divalent / Q000378:metabolism; D002459:Cell Fusion / Q000502:physiology*; D002463:Cell Membrane Permeability / Q000502:physiology; D002999:Clone Cells; D006863:Hydrogen-Ion Concentration; D008051:Lipid Bilayers / Q000378:metabolism; D008561:Membrane Fusion / Q000502:physiology; D012672:Semliki forest virus / Q000502:physiology*; D014759:Viral Envelope Proteins / Q000378:metabolism
D002413:Cations, Divalent; D008051:Lipid Bilayers; D014759:Viral Envelope Proteins