Growth and production kinetics of human x mouse and mouse hybridoma cells at reduced temperature and serum content.
The growth and production kinetics of a mouse hybridoma cell line and a human-mouse heterohybridoma were analyzed under conditions of reduced temperature and serum content. The mouse hybridoma P24 had a constant cell specific production rate and RNA content, while the heterohybridoma 3D6-LC4 showed growth associated production kinetics and an increased RNA content at higher growth rates. This behaviour of 3D6-LC4 cells can be explained by the unusual cell cycle kinetics of this line, which can be arrested in any phase under growth limiting conditions, so that a low growth rate does not result in a greater portion of high producing G1-phase cells. Substrate limitation changes the cell cycle distribution of this cell line to a greater extent than low temperature or serum content, which indicates that this stress factor exerts a greater physiological control than assumed.
Borth
N
N
Institute of Applied Microbiology, University of Agriculture, Vienna, Austria.
Heider
R
R
Assadian
A
A
Katinger
H
H
D000818:Animals; D001790:Blood Physiological Phenomena; D002455:Cell Division; D002460:Cell Line; D003470:Culture Media; D006801:Humans; D006825:Hybridomas / Q000166:cytology*; D007700:Kinetics; D051379:Mice; D012313:RNA / Q000032:analysis; D013696:Temperature
D003470:Culture Media; D012313:RNA