Interaction of the heat shock protein GroEL of Escherichia coli with single-stranded DNA-binding protein: suppression of ssb-113 by groEL46.
Previous studies from our laboratory have shown that an allele of the heat shock protein GroEL (groEL411) is able to specifically suppress some of the physiological defects of the single-stranded DNA-binding protein mutation ssb-1. A search for additional alleles of the groE genes which may act as suppressors for ssb mutations has led to the identification of groEL46 as a specific suppressor of ssb-113. It has very little or no effect on ssb-1 or ssb-3. All of the physiological defects of ssb-113, including temperature-sensitive growth, temperature-sensitive DNA synthesis, sensitivity to UV irradiation, methyl methanesulfonate, and bleomycin, and reduced recombinational capacity, are restored to wild-type levels. The ssb-113 allele, however, is unable to restore sensitivity of groEL46 cells to phage lambda. The mechanism of suppression of ssb-113 by groEL46 appears to differ from that of ssb-1 by groEL411. The data suggest that GroEL may interact with single-stranded DNA-binding protein in more than one domain.
Laine
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PS
Department of Biological Sciences, University of Cincinnati, Ohio 45221.
Meyer
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D001426:Bacterial Proteins / Q000235:genetics*; D001761:Bleomycin / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D018835:Chaperonin 10; D018834:Chaperonin 60; D004260:DNA Repair / Q000235:genetics; D004269:DNA, Bacterial / Q000096:biosynthesis; D004268:DNA-Binding Proteins / Q000235:genetics*; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics*; D016153:Genes, Suppressor / Q000235:genetics; D005816:Genetic Complementation Test; D006360:Heat-Shock Proteins / Q000235:genetics*; D006358:Hot Temperature; D008741:Methyl Methanesulfonate / Q000494:pharmacology; D009154:Mutation / Q000235:genetics*; D011995:Recombination, Genetic / Q000235:genetics; D013489:Suppression, Genetic; D014466:Ultraviolet Rays
D001426:Bacterial Proteins; D018835:Chaperonin 10; D018834:Chaperonin 60; D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; D006360:Heat-Shock Proteins; D001761:Bleomycin; D008741:Methyl Methanesulfonate