title: Rod opsin cDNA sequence from the sand goby (Pomatoschistus minutus) compared with those of other vertebrates.
abstract: The absorbance spectra of rods from the sand goby were measured by using microspectrophotometry. Analysis of the averaged spectra shows that the rod visual pigment has a maximum absorbance (lambda max) at approximately 501 nm. A sand goby retinal cDNA library was constructed and then screened with a partial sand goby rod opsin clone obtained by the polymerase chain reaction (PCR). The screening of the library yielded a full length rod opsin clone. The cDNA sequence and deduced amino acid sequence of this clone are compared with those of other vertebrate rod opsins.
authors.affiliation: Department of Biochemistry, University of Bristol, U.K.
authors.forename: S N
authors.initials: SN
authors.lastname: Archer
authors.forename: J N
authors.initials: JN
authors.lastname: Lythgoe
authors.forename: L
authors.initials: L
authors.lastname: Hall
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002645:Chickens; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D005136:Eye Proteins / Q000235:genetics*; D005399:Fishes / Q000235:genetics*; D015723:Gene Library; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D010786:Photoreceptor Cells / Q000502:physiology*; D016133:Polymerase Chain Reaction / Q000379:methods; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012160:Retina / Q000502:physiology; D017299:Rod Opsins; D012689:Sequence Homology, Nucleic Acid
chemical_list: D005136:Eye Proteins; D009838:Oligodeoxyribonucleotides; D017299:Rod Opsins; D012313:RNA; D004247:DNA