Galanin-evoked acetylcholine release in the rat striatum is blocked by the putative galanin antagonist M15.
The effect of the putative galanin (GAL) antagonist M15 on the Gal-evoked release of acetylcholine (ACh) was investigated in the rat striatum using microdialysis and HPLC techniques. GAL (3 nmol/10 microliters), applied in the lateral ventricle (i.c.v.) or perfused through the microdialysis membrane into the striatum, was found to enhance basal ACh release. The GAL-evoked release of striatal ACh was completely blocked by M15 (3 nmol/10 microliters), administered either i.c.v. or into the straitum. This finding suggests that GAL stimulates the basal ACh release in the rat striatum by a direct action of the peptide on striatal GAL receptors.
Pramanik
A
A
Department of Histology and Neurobiology, Karolinska Institute, Stockholm, Sweden.
Ogren
S O
SO
D000109:Acetylcholine / Q000378:metabolism*; D000818:Animals; D003342:Corpus Striatum / Q000187:drug effects* / Q000378:metabolism; D019004:Galanin / Q000031:analogs & derivatives; D008297:Male; D009479:Neuropeptides / Q000037:antagonists & inhibitors*; D010455:Peptides / Q000037:antagonists & inhibitors* / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D013373:Substance P / Q000031:analogs & derivatives*
D009479:Neuropeptides; D010455:Peptides; C074975:galantide; D013373:Substance P; D019004:Galanin; D000109:Acetylcholine