title: Rat KC cDNA cloning and mRNA expression in lung macrophages and fibroblasts.
abstract: We have isolated and sequenced overlapping cDNA clones for rat KC*. The 0.93 kb cDNA has a single open reading frame of 288 nucleotides, and substantial sequence identity with the platelet-factor 4 family members mouse KC, hamster gro, and human gro. Using cloned cDNA as a probe, expression of KC mRNA in lavaged rat alveolar macrophages (AMs) increased after lipopolysaccharide (LPS) treatment. We also studied expression in vitro by a rat fetal lung fibroblast cell line, RFL-6. Expression of KC mRNA in RFL-6 cells increased after treatment with interleukin 1 or with conditioned medium from rat AMs treated with LPS.
authors.affiliation: Department of Environmental Health, Harvard School of Public Health, Boston, Massachusetts 02115.
authors.forename: S
authors.initials: S
authors.lastname: Huang
authors.forename: J D
authors.initials: JD
authors.lastname: Paulauskis
authors.forename: L
authors.initials: L
authors.lastname: Kobzik
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002460:Cell Line; D002478:Cells, Cultured; D054360:Chemokine CXCL1; D018925:Chemokines; D019743:Chemokines, CXC; D003001:Cloning, Molecular; D016207:Cytokines / Q000235:genetics*; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D005347:Fibroblasts / Q000502:physiology; D015870:Gene Expression / Q000187:drug effects; D015723:Gene Library; D006133:Growth Substances / Q000235:genetics*; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins; D007375:Interleukin-1 / Q000494:pharmacology; D016676:Macrophages, Alveolar / Q000502:physiology*; D008969:Molecular Sequence Data; D005810:Multigene Family; D015345:Oligonucleotide Probes; D010978:Platelet Factor 4 / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000235:genetics*; D012194:RNA-Directed DNA Polymerase; D051381:Rats; D011994:Recombinant Proteins / Q000494:pharmacology; D012689:Sequence Homology, Nucleic Acid; D014162:Transfection
chemical_list: C475829:CXCL1 protein, human; D054360:Chemokine CXCL1; D018925:Chemokines; D019743:Chemokines, CXC; C475895:Cxcl1 protein, mouse; C513297:Cxcl1 protein, rat; D016207:Cytokines; D006133:Growth Substances; D036341:Intercellular Signaling Peptides and Proteins; D007375:Interleukin-1; D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D011994:Recombinant Proteins; C074302:keratinocyte-derived chemokines; D010978:Platelet Factor 4; D004247:DNA; D012194:RNA-Directed DNA Polymerase