The closely related homomeric and heterodimeric mannose-binding lectins from garlic are encoded by one-domain and two-domain lectin genes, respectively.
Lectin cDNA clones for two different lectins from garlic (Allium sativum L.) bulbs, ASAI and ASAII (ASA, Allium sativum agglutinin), were isolated and characterized. The first lectin, ASAI, is a heterodimer composed of two different subunits of 11.5 kDa and 12.5 kDa. It is translated from an mRNA of 1400 nucleotides encoding a polypeptide of 306 amino acids with two very similar domains. N-terminal sequencing of the two polypeptides of the mature lectin confirmed that both subunits are derived from the same precursor and that each corresponds to one of the two domains in the sequence. In contrast to ASAI, the second garlic lectin, ASAII, is a homodimer of two identical 12-kDa subunits. It is translated from an mRNA of approximately 800 nucleotides encoding a polypeptide of 154 amino acids. Interestingly, the coding region of the ASAII cDNA clones is almost identical to that of the second domain of the ASAI cDNA clones.
van Damme
E J
EJ
Laboratory for Phytopathology and Plant Protection, Catholic University of Leuven, Belgium.
Smeets
K
K
Torrekens
S
S
van Leuven
F
F
Goldstein
I J
IJ
Peumans
W J
WJ
D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D004247:DNA / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D005737:Garlic / Q000235:genetics*; D037102:Lectins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D008358:Mannose / Q000378:metabolism*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D037121:Plant Lectins; D010946:Plants, Medicinal; D011061:Poly A / Q000235:genetics; D016133:Polymerase Chain Reaction; D014176:Protein Biosynthesis; D012313:RNA / Q000235:genetics; D012333:RNA, Messenger
D037102:Lectins; D037121:Plant Lectins; D012333:RNA, Messenger; D011061:Poly A; D012313:RNA; D004247:DNA; D008358:Mannose