GLC-MS of reduced, acetylated, and methylated (2----4)- and (2----8)-linked disaccharides of 3-deoxy-D-manno-octulopyranosonic acid (Kdo).
Synthetic alpha- and beta-(2----4)- and alpha- and beta-(2----8)-linked disaccharides of 3-deoxy-D-manno-octulopyranosonic acid (Kdo), of which the synthesis of the beta-(2----4)-linked compound is described here, were used to develop a simple GLC-MS method for the determination of their anomeric configuration. The GLC and GLC-MS data for the reduced and acetylated or methylated derivatives of the above compounds indicate the alpha-linked synthetic disaccharides to be identical to those isolated from bacterial lipopolysaccharides.
Holst
O
O
Forschungsintitut Borstel, Institut für Experimentelle Biologie und Medizin, FRG.
Brade
H
H
Kosma
P
P
D000107:Acetylation; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D004187:Disaccharides / Q000737:chemistry* / Q000302:isolation & purification; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D007202:Indicators and Reagents; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D008969:Molecular Sequence Data; D015394:Molecular Structure; D010084:Oxidation-Reduction; D013400:Sugar Acids
D004187:Disaccharides; D007202:Indicators and Reagents; D013400:Sugar Acids; C002532:2-keto-3-deoxyoctonate