Characterization of human umbilical vein endothelial cell lines produced by transfection with the early region of SV40.
Human umbilical vein endothelial cells were transfected by electroporation with the plasmid pSV3neo, containing the early region of simian virus 40. The resultant "cell lines" divide rapidly (population doubling time of 33 h) for up to 24 passages in medium supplemented with 5% (v/v) serum and 2.5 micrograms/ml endothelial cell growth supplement. Several of these lines express basal levels of ICAM-1 and MHC class I but not MHC class II. One cell line, designated SGHEC-7, retained a number of differentiated endothelial cell functions throughout its lifespan. These functions include increased production of tissue plasminogen activator in response to histamine, thrombin, and PMA. Stability of function and rapid growth over 24 passages endow these cells with a number of advantages over primary cultures. The homogeneous cell population and consistency of response make them ideal for biochemical and immunological studies hereto impractical with primary human endothelial cells. The success of this approach may allow the production of functional cell lines from other vascular beds.
Fickling
S A
SA
Department of Cellular and Molecular Sciences, St. Georges Hospital Medical School, London, United Kingdom.
Tooze
J A
JA
Whitley
G S
GS
D015815:Cell Adhesion Molecules / Q000096:biosynthesis; D002455:Cell Division; D002460:Cell Line; D004730:Endothelium, Vascular / Q000166:cytology*; D006680:HLA Antigens / Q000096:biosynthesis; D006801:Humans; D018799:Intercellular Adhesion Molecule-1; D007375:Interleukin-1 / Q000494:pharmacology; D013539:Simian virus 40 / Q000235:genetics; D010959:Tissue Plasminogen Activator / Q000096:biosynthesis; D014162:Transfection; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology; D014471:Umbilical Veins / Q000166:cytology; D019010:Vascular Cell Adhesion Molecule-1
D015815:Cell Adhesion Molecules; D006680:HLA Antigens; D007375:Interleukin-1; D014409:Tumor Necrosis Factor-alpha; D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1; D010959:Tissue Plasminogen Activator