title: Induction of peroxisomal enzymes and a 64-kDa peptide in cultured mouse macrophages treated with clofibrate.
abstract: The presence of catalase-containing particles was demonstrated in mouse macrophages of the cell line J774.3. These cells were incubated with clofibrate, a hypolipidemic drug known to cause peroxisome proliferation in rodent hepatocytes. The specific activities of catalase and fatty acid oxidase were significantly increased after 48 h incubation with 2 mM clofibrate. This response proved to be time and dose dependent. Other organelle marker enzymes showed little change in activity. Unexpectedly, clofibrate treatment also produced an increase in a 64-kDa peptide in these cells.
authors.affiliation: Department of Cell and Molecular Biology, Pontificia Universidad Católica de Chile, Santiago.
authors.forename: A O
authors.initials: AO
authors.lastname: Couve
authors.forename: C
authors.initials: C
authors.lastname: Koenig
authors.forename: M J
authors.initials: MJ
authors.lastname: Santos
mesh_terms: D000818:Animals; D002460:Cell Line; D002994:Clofibrate / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel; D004790:Enzyme Induction / Q000187:drug effects; D005455:Fluorescent Antibody Technique; D007700:Kinetics; D008264:Macrophages / Q000187:drug effects / Q000201:enzymology*; D051379:Mice; D008830:Microbodies / Q000201:enzymology*
chemical_list: D002994:Clofibrate