Functional behaviour of dilute solutions of Hb-Kempsey: beta 99 (G-1) Asp--Asn.
The authors report that a diluted solution of Hb-Kempsey, beta 99 (G-1) Asp-Asn, can be chromatographically separated from the coexistent Hb-A and functionally examined if progressively depleted in O2 by bubbling pure nitrogen in the solution. Next, at fixed times, the O2 saturations of Hb are compared with the pO2s measured. Hb-Kempsey has a p50 of 1 torr, with an n-value of 1 and a Bohr effect of -0.2. Normal Hb-A of the same patient, examined with identical methods, presents: p50 = 4.5 torr; n = 2.7; Bohr effect = -0.412. Therefore, Hb-Kempsey is strongly hyperaffinic, does not display any heme-heme interaction, and has a half-normal Bohr effect.
Rabino Massa
E
E
Dip. Biologia animale, Università di Torino.
Ricco
G
G
Polimeni
C
C
Reddavid
M
M
D002850:Chromatography, Gel; D005260:Female; D006441:Hemoglobin A / Q000378:metabolism*; D006455:Hemoglobins, Abnormal / Q000302:isolation & purification / Q000378:metabolism*; D006579:Heterozygote; D006801:Humans; D006860:Hydrogen Bonding; D006863:Hydrogen-Ion Concentration; D009584:Nitrogen; D010100:Oxygen / Q000097:blood*; D010108:Oxyhemoglobins / Q000378:metabolism*; D010313:Partial Pressure
D006455:Hemoglobins, Abnormal; D010108:Oxyhemoglobins; C032577:hemoglobin Kempsey; D006441:Hemoglobin A; D009584:Nitrogen; D010100:Oxygen