[Rapid diagnosis of pulmonary Pseudomonas aeruginosa infections by indirect immunofluorescent antibody].
167 sputum specimens collected from patients with pulmonary infection were tested by IFA (Indirect immunofluorescent antibody-staining) with serogroup-specific monoclonal antibody to Pseudomonas aeruginosa, compared with quantitative sputum culture. The results showed the minimum concentration of bacteria detectable by IFA was 10 cfu/ml. 31(86.1%) were positive in 36 specimens with more than 10 cfu/ml of Pseudomonas aeruginosa. 22(100%) were positive in 22 specimens with more than 10 cfu/ml of Pseudomonas aeruginosa. In 127 specimens with a negative culture for Pseudomonas aeruginosa, 121(95.2%) were negative, 6(4.8%) were false-positive which could be identified by Gram's-staining. The type of Pseudomonas aeruginosa could also be identified within 3 hours.
Tang
Y C
YC
3rd Affiliated Hospital, Sun Yat-Sen.
D000911:Antibodies, Monoclonal; D005455:Fluorescent Antibody Technique; D006801:Humans; D011014:Pneumonia / Q000175:diagnosis* / Q000382:microbiology; D011552:Pseudomonas Infections / Q000175:diagnosis* / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000145:classification / Q000302:isolation & purification; D013183:Sputum / Q000382:microbiology
D000911:Antibodies, Monoclonal