Electrophoresis and silver staining of rat urinary proteins including alpha 2u-globulin (rat n II).
Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and silver staining have been used to monitor the fractionation of rat urinary proteins by Amicon Centriprep C30/C10 ultrafiltration and to assess the purification of alpha 2u-globulin, a laboratory animal allergen, precipitated by exhaustive dialysis. Factors influencing the protein composition of rat urine have been identified. Dialysis procedures have been evaluated for precipitation of alpha 2u-globulin and ultrafiltration used for further purification of the allergen.
Marshall
T
T
Biochemistry Research Laboratory, School of Pharmaceutical and Chemical Sciences, Sunderland Polytecnic, UK.
Williams
K M
KM
Bayard
C
C
Vesterberg
O
O
D000510:Alpha-Globulins / Q000652:urine*; D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D008297:Male; D011507:Proteinuria / Q000652:urine*; D051381:Rats; D011919:Rats, Inbred Strains; D016622:Silver Staining; D014462:Ultrafiltration
D000510:Alpha-Globulins; C013803:alpha 2u globulin