Expression and structure of serum gp70 as an acute phase protein in NZB mice.
A cDNA corresponding to a serum gp70 synthesized as an acute phase protein in mouse hepatocytes was cloned and analyzed. This cloned cDNA had the characteristics of an endogenous xenotropic murine leukemia virus. Synthesized oligo-DNA specific for this cDNA reacted strongly with liver RNA derived from NZB mice injected with LPS as a trigger of an acute phase inflammatory response. There was also low level of gp70 in the kidney in response to LPS injection. The LTR structure of the cDNA showed that this clone is the immediate precursor of an infectious xenotropic virus in the proposed evolutionary scheme of murine leukemia virus.
Shigemoto
K
K
Department of Molecular Pathology, Tokyo Metropolitan Institute of Gerontology, Japan.
Kubo
S
S
Itoh
Y
Y
Tate
G
G
Handa
S
S
Maruyama
N
N
D000209:Acute-Phase Proteins / Q000032:analysis*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D004247:DNA / Q000302:isolation & purification; D015342:DNA Probes; D015686:Gene Products, env / Q000032:analysis* / Q000235:genetics; D006023:Glycoproteins / Q000032:analysis* / Q000235:genetics; D009052:Leukemia Virus, Murine / Q000235:genetics*; D008070:Lipopolysaccharides; D008297:Male; D051379:Mice; D008814:Mice, Inbred NZB; D008969:Molecular Sequence Data; D012091:Repetitive Sequences, Nucleic Acid; D012191:Retroviridae Proteins / Q000032:analysis*; D014158:Transcription, Genetic
D000209:Acute-Phase Proteins; D015342:DNA Probes; D015686:Gene Products, env; D006023:Glycoproteins; D008070:Lipopolysaccharides; D012191:Retroviridae Proteins; D004247:DNA