The effect of L-carnitine on cholesterol metabolism in rat (Rattus bubalus) hepatocyte cells.
1. This paper concerns the study of the effect of L-carnitine on cholesterol metabolism in rat hepatocyte cells BRL-3A. In this research the binding of [125I]human low density lipoprotein (LDL) to BRL-3A cells and 3-hydroxy 3-methylglutaryl CoA reductase activity (HMG-CoA reductase activity) after L-carnitine incubation were studied. 2. It was found that L-carnitine is able to increase either the [125I]LDL binding or inhibit the HMG-CoA reductase activity in BRL-3A cells. 3. These results indicate that L-carnitine affects the cholesterol metabolism through an inhibition of HMG-CoA reductase activity that could be responsible for the increased [125I]LDL binding in rat hepatocytes.
Mondola
P
P
Istituto di Scienze Fisiologiche Umane, II Facoltà di Medicina e Chirurgia, Università di Napoli, Naples, Italy.
Santillo
M
M
De Mercato
R
R
Santangelo
F
F
D000818:Animals; D002331:Carnitine / Q000494:pharmacology*; D002460:Cell Line; D002784:Cholesterol / Q000378:metabolism*; D006801:Humans; D006903:Hydroxymethylglutaryl CoA Reductases / Q000378:metabolism; D007457:Iodine Radioisotopes; D008077:Lipoproteins, LDL / Q000097:blood; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D009115:Muridae / Q000378:metabolism*; D011485:Protein Binding; D051381:Rats
D007457:Iodine Radioisotopes; D008077:Lipoproteins, LDL; D002784:Cholesterol; D006903:Hydroxymethylglutaryl CoA Reductases; D002331:Carnitine