p53 mutations in formaldehyde-induced nasal squamous cell carcinomas in rats.
Formaldehyde induces squamous cell carcinomas in the nasal passages of rats following chronic inhalation exposure at concentrations of > or = 10 ppm. We have examined the complementary DNA of the tumor suppressor gene p53 from 11 primary formaldehyde-induced tumors for mutation using DNA sequence analysis. A polymerase chain reaction-amplified fragment of the rat p53 complementary DNA containing the evolutionarily conserved regions II-V was directly sequenced from each tumor. Point mutations in the p53 complementary DNA sequence were found in 5 of 11 of the tumors analyzed. These data demonstrate p53 point mutations in formaldehyde-induced squamous cell carcinomas and indicate a common alteration in certain rat and human squamous cell carcinomas of the respiratory tract.
Recio
L
L
Chemical Industry Institute of Toxicology, Research Triangle Park, North Carolina 27709.
Sisk
S
S
Pluta
L
L
Bermudez
E
E
Gross
E A
EA
Chen
Z
Z
Morgan
K
K
Walker
C
C
D000818:Animals; D001483:Base Sequence; D002294:Carcinoma, Squamous Cell / Q000139:chemically induced / Q000235:genetics*; D004273:DNA, Neoplasm / Q000032:analysis*; D005557:Formaldehyde; D016158:Genes, p53 / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics*; D009669:Nose Neoplasms / Q000139:chemically induced / Q000235:genetics*; D016133:Polymerase Chain Reaction; D051381:Rats; D011916:Rats, Inbred F344
D004273:DNA, Neoplasm; D005557:Formaldehyde