Insulin-like growth factor II stimulates glucose transport in human skeletal muscle.
We investigated the effect of insulin-like growth factor II (IGF-II) and insulin-like growth factor binding protein-1 (IGFBP-1) on 3-O-methylglucose transport in incubated human skeletal muscle strips. Increasing physiological concentrations of IGF-II stimulated glucose transport in a dose-dependent manner. Glucose transport was maximally stimulated in the presence of 100 ng/ml (13.4 nM) of IGF-II, which corresponded to the effect obtained by 100 microU/ml (0.6 nM) of insulin. Exposure of muscle strips to IGFBP-1 (500 ng/ml) inhibited the maximal effect of IGF-II on glucose transport by 40%. Thus, it is conceivable that IGF-II and IGFBP-1 are physiological regulators of the glucose transport process in human skeletal muscle.
Zierath
J R
JR
Department of Clinical Physiology, Karolinska Hospital, Karolinska Institute, Stockholm, Sweden.
Bang
P
P
Galuska
D
D
Hall
K
K
Wallberg-Henriksson
H
H
D000328:Adult; D001692:Biological Transport / Q000187:drug effects; D002352:Carrier Proteins / Q000494:pharmacology; D005947:Glucose / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D018969:Insulin-Like Growth Factor Binding Proteins; D007335:Insulin-Like Growth Factor II / Q000494:pharmacology*; D008297:Male; D009132:Muscles / Q000187:drug effects* / Q000378:metabolism; D013002:Somatomedins
D002352:Carrier Proteins; D018969:Insulin-Like Growth Factor Binding Proteins; D013002:Somatomedins; D007335:Insulin-Like Growth Factor II; D005947:Glucose