The induction of ornithine decarboxylase in human epidermis is independent of lipoxygenase and cyclo-oxygenase pathways.
In vivo studies in rodents suggest that prostaglandins and/or leukotrienes are involved in the epidermal induction of ornithine decarboxylase (ODC). Recently, we have shown that, in human epidermis, prostaglandins are not involved in this process. Here we report the role of leukotrienes in epidermal ODC induction in human skin. Topical flufenamic acid (Dignodolin), vehicle, or nothing was applied under plastic occlusion to three sites on the backs of healthy volunteers. This was followed 1 h later by Sellotape stripping. After renewed application and occlusion for 8 h, biopsies were carried out for the estimation of ODC levels. There were no significant differences in the levels of ODC between the flufenamic acid treated and control sites. To confirm this finding, test sites were irradiated with 3 MED of UVB. This was immediately followed by the application of flufenamic acid, vehicle, or nothing to the three irradiated sites. After 8 h, biopsies were taken, and the levels of ODC were again similar in the flufenamic acid- and the vehicle-treated sites. The data indicate that, following Sellotape stripping or UVB irradiation, neither lipoxygenase not cyclooxygenase products contribute to the in vivo induction of ODC in human epidermis.
Arnold
W P
WP
Department of Dermatology, University Hospital Nijmegen, The Netherlands.
Pennings
B J
BJ
van de Kerkhof
P C
PC
D000328:Adult; D016861:Cyclooxygenase Inhibitors / Q000494:pharmacology; D004790:Enzyme Induction / Q000187:drug effects / Q000528:radiation effects; D005260:Female; D005439:Flufenamic Acid / Q000494:pharmacology; D006801:Humans; D008084:Lipoxygenase / Q000502:physiology*; D016859:Lipoxygenase Inhibitors / Q000494:pharmacology; D008297:Male; D009955:Ornithine Decarboxylase / Q000096:biosynthesis*; D011451:Prostaglandin-Endoperoxide Synthases / Q000502:physiology*; D012867:Skin / Q000201:enzymology*; D014466:Ultraviolet Rays
D016861:Cyclooxygenase Inhibitors; D016859:Lipoxygenase Inhibitors; D005439:Flufenamic Acid; D008084:Lipoxygenase; D011451:Prostaglandin-Endoperoxide Synthases; D009955:Ornithine Decarboxylase