Characterization of the colorectal antigen IOR-C2.
A colorectal antigen (IOR-C2) was characterized by a monoclonal antibody produced against the colon cancer cell line SW1116. By immunohistochemical staining the antigen was abundant and strongly expressed in epithelium of normal colon whereas colorectal carcinomas showed a more variable and heterogenous reactivity to the antibody (IOR-C2). Radioimmunoprecipitates of SW1116 cell homogenates showed a 160-200 kD band in SDS gels. Physicochemical characterization indicate that at least two IOR-C2 reactive sites are present on the antigen tested and that it is mainly an 0-linked glycoprotein carbohydrate chain which can also be N-linked to the protein. The expression of IOR-C2 mimics that of the colon associated antigen (CAA) and NCC-CO-450 antigen but is distinct from these with regard to its expression in carcinomas as well as its physicochemical characteristics.
Vazquez
A M
AM
National Institute of Oncology, C. Habana, Cuba.
Tormo
B
B
Velandia
A
A
Giscombe
R
R
Ansotegui
I
I
Jeddi-Tehrani
M
M
Perez
R
R
Mellstedt
H
H
Biberfeld
P
P
D000818:Animals; D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor / Q000276:immunology; D015179:Colorectal Neoplasms / Q000276:immunology*; D000939:Epitopes; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000276:immunology; D051379:Mice; D014407:Tumor Cells, Cultured / Q000276:immunology
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D000939:Epitopes