Rapid morphological changes in bovine brain microvascular endothelial cells on extracellular matrices.
The morphological changes in endothelial cells derived from bovine brain microvasculature, carotid artery, and aorta during growth on extracellular matrices were compared. All cells formed tubular structures on a basement membrane. Ultrastructural studies showed that the tubular structures had lumens surrounded by many endothelial cells. On type I collagen gel, brain microvascular endothelial cells still formed tubular structures, but the other two cell types formed confluent monolayers. However, when a second layer of collagen gel was laid over these cells, tubular structures developed within 2-3 days. Brain microvascular endothelial cells form tubular structures more readily than endothelial cells derived from large vessels on both basement membrane and type I collagen gel.
Kaneko
T
T
Department of Neurosurgery, Faculty of Medicine, Kyoto University, Japan.
Nagata
I
I
Miyamoto
S
S
Kubo
H
H
Kikuchi
H
H
Fujisato
T
T
Ikada
Y
Y
D000818:Animals; D001921:Brain / Q000098:blood supply*; D002417:Cattle; D004730:Endothelium, Vascular / Q000473:pathology* / Q000648:ultrastructure; D005109:Extracellular Matrix / Q000473:pathology* / Q000648:ultrastructure; D008833:Microcirculation / Q000473:pathology / Q000648:ultrastructure; D008854:Microscopy, Electron; D009389:Neovascularization, Pathologic / Q000473:pathology