title: Sequence of the gene encoding type F neurotoxin of Clostridium botulinum.
abstract: Primers designed to conserved regions of botulinum and tetanus clostridial toxins were used to amplify DNA fragments from non-proteolytic Clostridium botulinum type F (202F) DNA using polymerase chain reaction technology. The fragments were cloned and the complete nucleotide sequence of the gene encoding type F toxin determined. Analysis of the nucleotide sequence demonstrated the presence of an open frame encoding a protein of 1274 amino acids, similar to other botulinum neurotoxins. Upstream of the toxin gene is the end of an open reading frame which encodes the C-terminus of a protein with homology to non-toxic-non-hemagglutinin component of type C progenitor toxin.
authors.affiliation: Department of Microbiology, AFRC Institute of Food Research, Reading, UK.
authors.forename: A K
authors.initials: AK
authors.lastname: East
authors.forename: P T
authors.initials: PT
authors.lastname: Richardson
authors.forename: D
authors.initials: D
authors.lastname: Allaway
authors.forename: M D
authors.initials: MD
authors.lastname: Collins
authors.forename: T A
authors.initials: TA
authors.lastname: Roberts
authors.forename: D E
authors.initials: DE
authors.lastname: Thompson
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D001905:Botulinum Toxins / Q000235:genetics*; D003001:Cloning, Molecular; D003014:Clostridium botulinum / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009498:Neurotoxins / Q000235:genetics*; D016366:Open Reading Frames; D017386:Sequence Homology, Amino Acid
chemical_list: D004269:DNA, Bacterial; D009498:Neurotoxins; D001905:Botulinum Toxins