title: Oxygen kinetics in preretinal perfluorotributylamine.
abstract: Previous studies have relied on various electrodes or probes to monitor preretinal oxygen tension in an effort to gain insight into retinal oxygenation. In order to corroborate and extend the results of such studies, we developed a relatively non-invasive method of determining preretinal oxygen tension using 19F nuclear magnetic resonance (NMR) spectroscopy. Small liquid perfluorocarbon (LPFC) droplets were injected into the preretinal vitreous space of the rabbit eye. The T1 value obtained from the fluorine nuclide could then be used to determine preretinal oxygen tension (PO2) with a high degree of sensitivity, since the fluorine spin-lattice relaxation rate (T1)-1 in LPFCs is directly proportional to PO2 under conditions of no flow and known temperature. In the present study, we investigated the oxygen uptake and clearance rates from small preretinal droplets of the LPFC perfluorotributylamine (FTBA) in response to step changes in arterial PO2. At all FTBA volumes examined (2, 10 and 100 microliters), the oxygen uptake and clearance curves were well approximated by a simple exponential equation with mean time constants 9.8/15.3, 21.4/19.4 and 77.7/45.3 min (uptake/clearance), respectively. Following return to normoxemic (baseline) conditions, FTBA droplets provided a preretinal PO2 of 39.4 +/- 9.2 mmHg (mean +/- S.D., n = 12). The 19F NMR method provides a measure of steady-state preretinal PO2 that independently verifies and complements information obtained using oxygen-sensitive microelectrodes or probes. However, the long time constants for oxygen uptake and clearance, particularly in FTBA volumes on the order of 10 microliters and greater, may represent a practical limitation of this method for determining rapid oxygen flux in the preretinal vitreous space.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Ophthalmology, Duke University, Durham, NC 27710.
authors.forename: C A
authors.initials: CA
authors.lastname: Wilson
authors.forename: B A
authors.initials: BA
authors.lastname: Berkowitz
authors.forename: D L
authors.initials: DL
authors.lastname: Hatchell
mesh_terms: D000818:Animals; D005466:Fluorocarbons / Q000493:pharmacokinetics; D009682:Magnetic Resonance Spectroscopy; D008722:Methods; D010100:Oxygen / Q000097:blood; D010101:Oxygen Consumption / Q000502:physiology*; D011817:Rabbits; D012160:Retina / Q000378:metabolism*; D013997:Time Factors; D014822:Vitreous Body / Q000378:metabolism
chemical_list: D005466:Fluorocarbons; C008807:perfluorotributylamine; D010100:Oxygen