Colony morphotype on Sabouraud-triphenyltetrazolium agar: a simple and inexpensive method for Candida subspecies discrimination.
A new method of Candida subspecies discrimination on Sabouraud-triphenyltetrazolium agar is reported. Five hundred sixty-two strains of Candida and Torulopsis glabrata, previously identified by conventional mycological methods, were studied. Each strain received a three-letter code and a number based on its colonial morphology. Sixteen morphotypes were found for Candida albicans, 6 were found for Candida parapsilosis, 4 were found for both Candida guilliermondii and Candida krusei, and 12 were found for Candida tropicalis. None of the 56 T. glabrata strains studied grew on this agar. A reproducibility of 95% was found for C. albicans. The simplicity and low cost could make this method useful for typing Candida spp.
Quindós
G
G
Departamento de Microbiología e Inmunología, Facultad de Medicina y Odontología, Universidad del País Vasco, Bilbao, Spain.
Fernández-Rodríguez
M
M
Burgos
A
A
Tellaetxe
M
M
Cisterna
R
R
Pontón
J
J
D000362:Agar; D002175:Candida / Q000145:classification* / Q000254:growth & development; D016533:Mycological Typing Techniques; D015203:Reproducibility of Results; D013778:Tetrazolium Salts
D013778:Tetrazolium Salts; C009591:triphenyltetrazolium; D000362:Agar