Effects of macrophage colony-stimulating factor on the activities of murine monocytes and peritoneal macrophages in vivo.
The ability of peripheral blood monocytes, granulocytes and resident peritoneal macrophages to generate hydrogen peroxide in response to concanavalin A was enhanced by a single intravenous injection of macrophage colony-stimulating factor (M-CSF) of recombinant human type in AKR mice. In response to phorbol myristate acetate, only granulocytes and resident peritoneal macrophages showed the enhanced ability. Phagocytosis by those cells was not stimulated by M-CSF. Surface marker analysis showed an increased expression of F4/80 and Mac1 on monocytes, Mac1 expression on granulocytes and LFA-1 expression on resident peritoneal macrophages. Ia antigen on resident peritoneal macrophages was suppressed by M-CSF. M-CSF can induce monocytes, granulocytes and resident peritoneal macrophages to generate hydrogen peroxide and enhances their maturation in vivo.
Katsura
Y
Y
Department of Microbiology, National Defense Medical College, Saitama, Japan.
Tsuru
S
S
Noritake
M
M
Shinomiya
N
N
Kayashima
S
S
Motoyoshi
K
K
Rokutanda
M
M
D000818:Animals; D000954:Antigens, Surface / Q000276:immunology; D005434:Flow Cytometry; D006098:Granulocytes / Q000276:immunology; D016173:Macrophage Colony-Stimulating Factor / Q000276:immunology*; D008264:Macrophages / Q000276:immunology*; D008297:Male; D051379:Mice; D008806:Mice, Inbred AKR; D009000:Monocytes / Q000276:immunology*; D010529:Peritoneal Cavity; D010587:Phagocytosis / Q000276:immunology; D011994:Recombinant Proteins / Q000276:immunology
D000954:Antigens, Surface; D011994:Recombinant Proteins; D016173:Macrophage Colony-Stimulating Factor