Microbiologically monitored fumigation of a newly built SPF laboratory rodent facility.
The initial sanitization and sterilization of a newly built animal facility for the breeding and holding of specific pathogen free (SPF) rats and mice is described. The fumigation programme was started with methyl bromide treatment directed primarily against arthropods, followed by ammonia spray to kill coccidial oocysts and concluded by three formaldehyde treatments with fog and spray against bacteria and viruses. The practicalities and problems involved are described in detail and the rationale and purpose of the programme and its monitoring are discussed. The report is expected to contribute towards the establishment of a rational, efficient and standardized fumigation programme for SPF animal facilities, under increasing constraints of safety and environmental considerations concerning pollution with toxic and corrosive agents.
Sebesteny
A
A
FRAR SpA San Pietro al Natisone, Udine, Italy.
Milite
G
G
Martelossi
P
P
D000818:Animals; D000830:Animals, Laboratory / Q000382:microbiology*; D001412:Bacillus subtilis / Q000187:drug effects; D002176:Candida albicans / Q000187:drug effects; D004784:Environmental Monitoring / Q000379:methods*; D005557:Formaldehyde / Q000009:adverse effects / Q000494:pharmacology; D005651:Fumigation / Q000009:adverse effects / Q000379:methods*; D006799:Housing, Animal; D006842:Hydrocarbons, Brominated / Q000009:adverse effects / Q000494:pharmacology; D051379:Mice; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D051381:Rats; D013047:Specific Pathogen-Free Organisms; D013171:Spores, Bacterial / Q000187:drug effects; D013211:Staphylococcus aureus / Q000187:drug effects
D006842:Hydrocarbons, Brominated; D005557:Formaldehyde; C005218:methyl bromide