Three additional inositol 1,4,5-trisphosphate receptors: molecular cloning and differential localization in brain and peripheral tissues.
Three inositol 1,4,5-trisphosphate receptor (IP3R) cDNAs, designated IP3R-II, -III, and -IV, were cloned from a mouse placenta cDNA library. All three display strong homology in membrane-spanning domains M7 and M8 to the originally cloned cerebellar IP3R-I, with divergences predominantly in cytoplasmic domains. Levels of mRNA for the three additional IP3Rs in general are substantially lower than for IP3R-I, though in the gastrointestinal tract the levels of IP3R-III may be comparable to IP3R-I. Cerebellar Purkinje cells express at least two and possibly three distinct IP3Rs, suggesting heterogeneity of IP3 action within a single cell.
Ross
C A
CA
Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205-2195.
Danoff
S K
SK
Schell
M J
MJ
Snyder
S H
SH
Ullrich
A
A
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D001921:Brain / Q000502:physiology*; D015220:Calcium Channels; D002462:Cell Membrane / Q000378:metabolism; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D014644:Genetic Variation; D005865:Gestational Age; D015544:Inositol 1,4,5-Trisphosphate / Q000378:metabolism; D053496:Inositol 1,4,5-Trisphosphate Receptors; D051379:Mice; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009838:Oligodeoxyribonucleotides; D009928:Organ Specificity; D010920:Placenta / Q000502:physiology; D011247:Pregnancy; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D011956:Receptors, Cell Surface / Q000235:genetics*; D018160:Receptors, Cytoplasmic and Nuclear; D012689:Sequence Homology, Nucleic Acid
D015220:Calcium Channels; D053496:Inositol 1,4,5-Trisphosphate Receptors; D009838:Oligodeoxyribonucleotides; D011956:Receptors, Cell Surface; D018160:Receptors, Cytoplasmic and Nuclear; D012313:RNA; D015544:Inositol 1,4,5-Trisphosphate; D004247:DNA