Isolation of a human cDNA encoding a 25 kDa FK-506 and rapamycin binding protein.
Recently, the nearly complete peptide sequence of a 25 kDa rapamycin and FK-506 binding protein that had been isolated from calf thymus, brain, and spleen was reported (1). Based upon the amino acid sequence of this bovine protein, bFKBP25, we have isolated from a JURKAT cDNA library the cDNA encoding the human homolog, hFKBP25. Translation of the open reading frame contained within this cDNA clone yields a sequence that, in its C-terminal half, is 41% identical to the major human FK-506 binding protein, hFKBP12, and 43% identical to hFKBP13. The N-terminal half of hFKBP25 is unrelated to any known protein.
Wiederrecht
G
G
Department of Immunology Research, Merck Research Labs, Rahway, New Jersey 07065.
Martin
M M
MM
Sigal
N H
NH
Siekierka
J J
JJ
D000595:Amino Acid Sequence; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D004277:DNA, Single-Stranded / Q000235:genetics; D006360:Heat-Shock Proteins / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D007166:Immunosuppressive Agents / Q000378:metabolism*; D008214:Lymphocytes / Q000502:physiology; D008969:Molecular Sequence Data; D011090:Polyenes / Q000378:metabolism*; D012689:Sequence Homology, Nucleic Acid; D020123:Sirolimus; D016559:Tacrolimus / Q000378:metabolism*; D022021:Tacrolimus Binding Proteins
D002352:Carrier Proteins; D004277:DNA, Single-Stranded; D006360:Heat-Shock Proteins; D007166:Immunosuppressive Agents; D011090:Polyenes; D022021:Tacrolimus Binding Proteins; D020123:Sirolimus; D016559:Tacrolimus