title: Profile of renal permselectivity by simultaneous enzyme-linked immunosorbent assay of albumin, transferrin, IgG, and alpha 1-microglobulin with a new microplate reader.
abstract: A competitive enzyme-linked immunosorbent assay (ELISA) is described for determining a renal permselectivity profile involving the urinary proteins albumin, transferrin, IgG, and alpha 1-microglobulin (alpha 1-m). The ELISA reader uses a computer-controlled array of multiplexed light-emitting diode (LED)-photodiode pairs for rapid measurements of absorbance on microplates. A 3,3'-dimethylnaphthidine reagent adapts the 3,5,3',5'-tetramethylbenzidine chromophore to monochromatic LED emission at 550 nm. We applied this ELISA to the determination of renal permselectivity in healthy children and young adults and in children with insulin-dependent diabetes mellitus. The geometric means (and SD) of protein excretion rates in a group of 85 normal subjects were as follows: albumin, 3.5 micrograms/min (1.83); transferrin, 173 ng/min (2.76); IgG, 1.11 micrograms/min (2.22), and alpha 1-m, 0.98 microgram/min (2.36).
authors.affiliation: Department of Pediatrics, University of Cincinnati, OH 45267-0585.
authors.forename: R A
authors.initials: RA
authors.lastname: Magnotti
authors.forename: J P
authors.initials: JP
authors.lastname: Eberly
authors.forename: P R
authors.initials: PR
authors.lastname: Khoury
authors.forename: S R
authors.initials: SR
authors.lastname: Daniels
authors.forename: D J
authors.initials: DJ
authors.lastname: Drozda
authors.forename: A M
authors.initials: AM
authors.lastname: Turner
authors.forename: L M
authors.initials: LM
authors.lastname: Dolan
mesh_terms: D000293:Adolescent; D000328:Adult; D000419:Albuminuria / Q000652:urine*; D000510:Alpha-Globulins / Q000652:urine*; D002648:Child; D002675:Child, Preschool; D003922:Diabetes Mellitus, Type 1 / Q000652:urine*; D003928:Diabetic Nephropathies / Q000652:urine*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods* / Q000706:statistics & numerical data; D006801:Humans; D007074:Immunoglobulin G / Q000652:urine*; D007202:Indicators and Reagents; D014168:Transferrin / Q000652:urine*
chemical_list: D000510:Alpha-Globulins; D007074:Immunoglobulin G; D007202:Indicators and Reagents; D014168:Transferrin; C016001:alpha-1-microglobulin