Effect of thyroid hormone status on the expression of the mRNAs of components of the lipolytic regulatory cascade in brown adipose tissue.
1. The levels of mRNAs for RII beta and G beta were about 50% lower in brown adipose tissue (BAT) from hyperthyroid than from hypothyroid rats. 2. Treatment of hypothyroid rats with T3 resulted in a 50% decrease in mRNAs for RII beta and G beta in BAT occurring by 12 hr after treatment. 3. The levels of mRNAs for hormone-sensitive lipase, G alpha s and C alpha in BAT were unchanged by thyroid hormone status. 4. The results suggest that thyroid hormone may be involved in negative regulation of the expression of RII beta and G beta at the transcriptional level in BAT.
Thompson
M P
MP
Department of Medicine, University of Minnesota, Minneapolis 55455.
Strait
K A
KA
D002001:Adipose Tissue, Brown / Q000502:physiology*; D000818:Animals; D015152:Blotting, Northern; D015342:DNA Probes; D019204:GTP-Binding Proteins / Q000235:genetics; D008066:Lipolysis / Q000235:genetics*; D008297:Male; D011494:Protein Kinases / Q000235:genetics; D012313:RNA / Q000302:isolation & purification; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D002787:Sterol Esterase / Q000235:genetics; D013963:Thyroid Hormones / Q000502:physiology*
D015342:DNA Probes; D012333:RNA, Messenger; D013963:Thyroid Hormones; D012313:RNA; D011494:Protein Kinases; D002787:Sterol Esterase; D019204:GTP-Binding Proteins