Accelerated expression of secreted alpha-chain gene in anaphylactoid purpura.
The mechanisms of the elevation of serum IgA levels in anaphylactoid purpura were investigated. Serum IgA levels were significantly elevated within 2 weeks (5 to 14 days for all 12 patients) after onset in patients with anaphylactoid purpura. Serum IgM and IgG were not elevated. Although the percentages of surface IgA-bearing cells were not increased in the patients, the numbers of IgA-secreting cells within 2 weeks after onset in the patients with anaphylactoid purpura were significantly higher than those of controls. In northern blot analysis on lymphocytes, the secreted alpha (alpha s)-chain gene was well expressed compared with the membrane-bound alpha (alpha m)-chain gene, within 2 weeks after the onset of anaphylactoid purpura. Therefore, stimulation by a certain agent or a certain immune response may accelerate expression of the alpha s-chain gene in anaphylactoid purpura.
Kondo
N
N
Department of Pediatrics, Gifu University School of Medicine, Japan.
Kasahara
K
K
Shinoda
S
S
Orii
T
T
D015152:Blotting, Northern; D002648:Child; D002675:Child, Preschool; D015342:DNA Probes; D006801:Humans; D011695:IgA Vasculitis / Q000235:genetics / Q000276:immunology*; D007070:Immunoglobulin A / Q000097:blood*; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000032:analysis
D015342:DNA Probes; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D012333:RNA, Messenger