Immunological effects of hemoglobin, encapsulated hemoglobin, polyhemoglobin and conjugated hemoglobin using different immunization schedules.
Repeated subcutaneous immunizing injections into rats of Freund's adjuvant containing rat hemoglobin or o-raffinose rat polyhemoglobin did not result in increase in IgG antibody titers. However, heterologous hemoglobin injected as above is antigenic (49.50 + 6.70 % CPM). Crosslinking heterologous hemoglobin into o-raffinose polyhemoglobin further increased its antigenicity (75.60 + 4.08). Thus, unlike homologous hemoglobin, cross-linking of heterologous hemoglobin increased its antigenicity. Liposome encapsulated homologous hemoglobin injected subcutaneously with or without Freund's adjuvant did not show antigenicity. Encapsulated heterologous hemoglobin resulted in a minimal increase in antibody titers (10.73 + 4.64) only with Freund's adjuvant, but no increase when injected without Freund's adjuvant. Conjugated heterologous hemoglobin (PEG-Hb) injected intravenously by itself at weekly intervals did not result in significant increase in antibody titers on the 5th week.
Chang
T M
TM
Artificial Cells and Organs Research Centre, Faculty of Medicine, McGill University Montreal, Quebec, Canada.
Lister
C
C
Nishiya
Varma
R
R
D000818:Animals; D000917:Antibody Formation; D001802:Blood Substitutes / Q000008:administration & dosage / Q000302:isolation & purification / Q000633:toxicity*; D003432:Cross-Linking Reagents; D005620:Freund's Adjuvant / Q000008:administration & dosage; D006454:Hemoglobins / Q000008:administration & dosage / Q000276:immunology* / Q000633:toxicity; D007115:Immunization Schedule; D007279:Injections, Subcutaneous; D008081:Liposomes; D011092:Polyethylene Glycols / Q000008:administration & dosage / Q000633:toxicity; D011887:Raffinose; D051381:Rats; D017207:Rats, Sprague-Dawley
D001802:Blood Substitutes; D003432:Cross-Linking Reagents; D006454:Hemoglobins; D008081:Liposomes; C037158:polyhemoglobin; C050907:pyridoxalated-hemoglobin-polyethylene glycol conjugate; D011092:Polyethylene Glycols; D005620:Freund's Adjuvant; D011887:Raffinose