Phospholipases introduced into the hypophase affect the surfactant film outlining a bubble.
The hypophase exchanger is a recently developed device that makes it possible to replace the liquid in the sample chamber of a pulsating bubble surfactometer, after a bubble has been formed, without changing the size of the bubble. A surfactant film outlining the bubble will retain its surface properties, provided the liquid entering the sample chamber and replacing the hypophase is inert. If, on the other hand, the new hypophase consists of a phospholipase solution, the physical properties of the film are seriously affected. It was found that when phospholipase C, even at low concentration, entered the sample chamber, the physical properties were significantly changed. Phospholipase A2 had to be added at a higher concentration to exert a similar effect. It is postulated that the site of action of phospholipase A2 may be partly protected in the hydrophobic region of the tightly packed surfactant film.
Enhorning
G
G
Department of Gynecology/Obstetrics, State University of New York, Buffalo.
Shumel
B
B
Keicher
L
L
Sokolowski
J
J
Holm
B A
BA
D000818:Animals; D055592:Biophysical Phenomena; D001703:Biophysics; D002417:Cattle; D066298:In Vitro Techniques; D010740:Phospholipases / Q000378:metabolism*; D010741:Phospholipases A / Q000378:metabolism; D054467:Phospholipases A2; D011650:Pulmonary Alveoli / Q000378:metabolism; D011654:Pulmonary Edema / Q000378:metabolism; D011663:Pulmonary Surfactants / Q000737:chemistry / Q000378:metabolism*; D013499:Surface Properties; D010738:Type C Phospholipases / Q000378:metabolism
D011663:Pulmonary Surfactants; D010740:Phospholipases; D010741:Phospholipases A; D054467:Phospholipases A2; D010738:Type C Phospholipases