Constitutive protein secretion by guinea-pig seminal vesicle epithelial cells.
1. Secretion of pulse-labelled protein by the isolated epithelium of guinea-pig seminal vesicle epithelium was rapid, unaffected by cholinergic and adrenergic drugs, cyclic nucleotides or changes in the sodium, potassium and calcium concentrations of the "chase" medium. 2. Low temperature, NH4Cl, hyper- and hypo-osmolarity and membrane-stabilizing agents inhibited secretion which was also dependent on aerobic metabolism. 3. Monensin reduced secretion of the six labelled, relatively low molecular weight proteins recovered from the medium in a concentration-dependent, apparently non-specific manner.
Hudson
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DA
Cell and Developmental Biology Research Unit, School of Biological Sciences, Victoria University of Wellington, New Zealand.
D000818:Animals; D004847:Epithelial Cells; D004848:Epithelium / Q000187:drug effects / Q000378:metabolism; D006168:Guinea Pigs / Q000502:physiology*; D008297:Male; D008854:Microscopy, Electron; D011506:Proteins / Q000302:isolation & purification / Q000378:metabolism*; D012669:Seminal Vesicles / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*
D011506:Proteins