title: Microbiological evaluation of the efficacy of chlorhexidine in a sustained-release device for dentine sterilization.
abstract: The aim of this in-vitro study was to evaluate the effect of chlorhexidine in solution and in a sustained-release device as an intracanal medication. Hollow cylindrical dentine specimens prepared from bovine incisors were incubated with Streptococcus faecalis for a period of 3 weeks. The intracanal medicaments tested were 0.2% chlorhexidine gluconate solution (CH), chlorhexidine in a sustained-release device (1.2 mg) (SBD), camphorated paramonochlorophenol (CMCP), and a control (no medication). Each medicament was introduced into the lumen of one dentine specimen and incubated for 5 min, 24 h, 48 h or 7 days at 37 degrees C. The bacteriological samples were taken by shaving the dentine inside the lumen with dental burs ranging in size from ISO 023-033. The dentine powder was collected in test-tubes containing growth medium and incubated for 24 h. The optical density of the medium was recorded by means of a spectrophotometer at a wavelength of 540 nm. There was a statistically significant difference between the control group and all the medicaments tested.
authors.affiliation: The Hebrew University-Hadassah School of Dental Medicine, Jerusalem.
authors.forename: I
authors.initials: I
authors.lastname: Heling
authors.forename: M
authors.initials: M
authors.lastname: Sommer
authors.forename: D
authors.initials: D
authors.lastname: Steinberg
authors.forename: M
authors.initials: M
authors.lastname: Friedman
authors.forename: M N
authors.initials: MN
authors.lastname: Sela
mesh_terms: D000818:Animals; D002164:Camphor / Q000627:therapeutic use; D002417:Cattle; D002710:Chlorhexidine / Q000008:administration & dosage / Q000494:pharmacology*; D002733:Chlorophenols / Q000494:pharmacology; D015169:Colony Count, Microbial; D003692:Delayed-Action Preparations; D003804:Dentin; D013293:Enterococcus faecalis / Q000187:drug effects*; D012388:Root Canal Irrigants / Q000494:pharmacology*
chemical_list: D002733:Chlorophenols; D003692:Delayed-Action Preparations; D012388:Root Canal Irrigants; D002164:Camphor; D002710:Chlorhexidine