Distribution of surface-membrane molecules on bone marrow and cord blood CD34+ hematopoietic cells.
We have investigated the distribution of membrane molecules on CD34+ hematopoietic cells isolated from human bone marrow (BM) and cord blood (CB). A distinct CD10+ population was present in BM, but it was not detected in CB. Most CD34+ CD10+ cells in BM were B-cell precursors (BCP), because they expressed CD19. However, CD40 and CD37 were found on the majority of CD34+ cells from either BM or CB, demonstrating that these antigens are not restricted to B-lineage CD34+ cells. CD40 and CD37 were lost during culture of CD34+ cells in the presence of interleukin 3 (IL-3), indicating transient expression early in myeloid development. CD13 antigen was detected on virtually all CD34+ cells from BM and CB. Accordingly, CD13 was present on CD34+ CD10+ cells, demonstrating that this structure is not restricted to myeloid CD34+ cells. In contrast, myeloid CD33 antigen was not detected on CD34+ CD10+ cells. Expression levels of CD13 and of CD33 were heterogeneous in BM, reflecting diversity within the resident CD34+ population. CD25 and CD71 were found on a proportion of CD34+ cells from either BM or CB and maintained during culture in IL-3, consistent with a distribution on activated cells. Finally, a variety of adhesion receptors were present on CD34+ cells. These included the alpha 4 beta 1 (VLA-4), alpha 5 beta 1 (VLA-5), and alpha L beta 2 (LFA-1) integrins, as well as ICAM-1, LFA-3, H-CAM, and LAM-1. Expression of adhesion receptors was remarkably similar in BM and CB, and it followed an all-or-nothing pattern that failed to delineate CD34+ subsets. Taken together, our data show that although CD34+ cells from BM constitute a more heterogeneous population, resident and circulating CD34+ cells largely display the same cell-surface molecules.
Saeland
S
S
Schering-Plough Laboratory for Immunological Research, Dardilly, France.
Duvert
V
V
Caux
C
C
Pandrau
D
D
Favre
C
C
Vallé
A
A
Durand
I
I
Charbord
P
P
de Vries
J
J
Banchereau
J
J
D015703:Antigens, CD / Q000032:analysis*; D018941:Antigens, CD19; D018952:Antigens, CD34; D000944:Antigens, Differentiation, B-Lymphocyte / Q000032:analysis; D015214:Antigens, Differentiation, Myelomonocytic / Q000032:analysis; D000954:Antigens, Surface / Q000032:analysis*; D001853:Bone Marrow / Q000737:chemistry* / Q000648:ultrastructure; D001854:Bone Marrow Cells; D018826:CD13 Antigens; D019013:CD40 Antigens; D015815:Cell Adhesion Molecules / Q000032:analysis; D002478:Cells, Cultured; D005312:Fetal Blood / Q000737:chemistry* / Q000166:cytology*; D005434:Flow Cytometry; D006412:Hematopoietic Stem Cells / Q000737:chemistry* / Q000276:immunology* / Q000648:ultrastructure; D006801:Humans; D016023:Integrins / Q000032:analysis; D018799:Intercellular Adhesion Molecule-1; D007377:Interleukin-3 / Q000494:pharmacology; D008565:Membrane Proteins / Q000032:analysis*; D015375:Receptors, Interleukin-2 / Q000032:analysis; D011990:Receptors, Transferrin; D016029:Receptors, Very Late Antigen / Q000032:analysis; D063268:Sialic Acid Binding Ig-like Lectin 3
D015703:Antigens, CD; D018941:Antigens, CD19; D018952:Antigens, CD34; D000944:Antigens, Differentiation, B-Lymphocyte; D015214:Antigens, Differentiation, Myelomonocytic; D000954:Antigens, Surface; C571163:CD33 protein, human; D019013:CD40 Antigens; C068126:CD71 antigen; D015815:Cell Adhesion Molecules; D016023:Integrins; D007377:Interleukin-3; D008565:Membrane Proteins; D015375:Receptors, Interleukin-2; D011990:Receptors, Transferrin; D016029:Receptors, Very Late Antigen; D063268:Sialic Acid Binding Ig-like Lectin 3; D018799:Intercellular Adhesion Molecule-1; D018826:CD13 Antigens