Toxicity of chemically generated nitric oxide towards pancreatic islet cells can be prevented by nicotinamide.
Previous studies have indicated that nitric oxide is involved in the lysis of pancreatic islet cells by inflammatory macrophages. Here we show that the incubation of islet cells with chemical NO-donors leads to cell lysis in a concentration and time dependent way. Islet cell death could be prevented by nicotinamide and 3-aminobenzamide, which are known to inhibit ADP-ribosylation, while several scavengers of oxygen radicals, N-acetylcysteine, dihydrolipoic acid, dimethylthiourea and citiolone, provided no protection.
Kallmann
B
B
Diabetes Research Institute University of Düsseldorf, Germany.
Burkart
V
V
Kröncke
K D
KD
Kolb-Bachofen
V
V
Kolb
H
H
D000111:Acetylcysteine / Q000494:pharmacology; D000818:Animals; D001549:Benzamides / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D016166:Free Radical Scavengers; D066298:In Vitro Techniques; D007515:Islets of Langerhans / Q000166:cytology* / Q000187:drug effects; D007700:Kinetics; D009536:Niacinamide / Q000494:pharmacology*; D009569:Nitric Oxide / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D009599:Nitroprusside / Q000494:pharmacology*; D010396:Penicillamine / Q000031:analogs & derivatives / Q000494:pharmacology; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; D051381:Rats; D011919:Rats, Inbred Strains; D026423:S-Nitroso-N-Acetylpenicillamine; D012966:Sodium Cyanide / Q000494:pharmacology*; D008063:Thioctic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D013884:Thiosulfate Sulfurtransferase / Q000378:metabolism / Q000494:pharmacology; D014665:Vasodilator Agents / Q000494:pharmacology
D001549:Benzamides; D016166:Free Radical Scavengers; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; D014665:Vasodilator Agents; D009599:Nitroprusside; D009536:Niacinamide; D009569:Nitric Oxide; D008063:Thioctic Acid; D026423:S-Nitroso-N-Acetylpenicillamine; C015144:dihydrolipoic acid; C025160:3-aminobenzamide; D013884:Thiosulfate Sulfurtransferase; D010396:Penicillamine; D012966:Sodium Cyanide; D000111:Acetylcysteine