Intracerebroventricularly administered pertussis toxin blocks the central vasopressor action of neuropeptide Y(13-36) in the awake unrestrained male rat.
The effects of intracerebroventricular (i.v.t.) injections of pertussis toxin (PTX) (10 micrograms/30 microliters, 48 h) were studied on the cardiovascular actions of i.v.t. administered neuropeptide Y(13-36) (NPY(13-36)) as evaluated in the awake unrestrained male rat. The vasopressor action of NPY(13-36) in the peak dose of 3000 pmol per rat was significantly inhibited by pretreatment by PTX. Pertussis toxin treatment alone significantly reduced the baroreceptor reflex elicited by L-phenylephrine. The results are compatible with the view that G-proteins mediate the central vasopressor actions of NPY(13-36) and thus probably are involved in NPY Y2-receptor transduction in cardiovascular areas of the brainstem.
Narváez
J A
JA
Department of Histology and Neurobiology, Karolinska Institutet, Stockholm, Sweden.
Aguirre
J A
JA
Van der Ploeg
I
I
Fuxe
K
K
D000818:Animals; D001794:Blood Pressure / Q000187:drug effects*; D001921:Brain / Q000187:drug effects; D006321:Heart / Q000187:drug effects; D006339:Heart Rate / Q000187:drug effects; D007276:Injections, Intraventricular; D008297:Male; D009478:Neuropeptide Y / Q000037:antagonists & inhibitors*; D010446:Peptide Fragments / Q000037:antagonists & inhibitors*; D037342:Pertussis Toxin; D010656:Phenylephrine / Q000494:pharmacology; D011311:Pressoreceptors / Q000187:drug effects / Q000502:physiology; D051381:Rats; D011919:Rats, Inbred Strains; D012018:Reflex / Q000187:drug effects; D013047:Specific Pathogen-Free Organisms; D010566:Virulence Factors, Bordetella / Q000008:administration & dosage / Q000494:pharmacology*
D009478:Neuropeptide Y; D010446:Peptide Fragments; D010566:Virulence Factors, Bordetella; C062924:neuropeptide Y (13-36); D010656:Phenylephrine; D037342:Pertussis Toxin