Rapid determination of propyphenazone in plasma by high-performance liquid chromatography.
A rapid and simple high-performance liquid chromatographic assay for the determination of propyphenazone in plasma is described. Phenylbutazone was used as the internal standard. Plasma proteins were precipitated with acetonitrile before injection onto a 3-microns Supelcosil LC-18 column. The mobile phase, ethanol containing 0.2% (v/v) heptylamine-0.005 M potassium dihydrogenphosphate (30:70, v/v), was used at a flow-rate of 1.3 ml/min. The quantitation was performed by ultraviolet detection at a wavelength of 270 nm. The chromatographic time was 7 min. The within- and between-day coefficients of variation were less than 6% and the recoveries close to 100% for concentrations between 0.4 and 22 mumol/l. The limit of quantitation was 0.4 mumol/l (ca. 100 ng/ml).
Rouan
M C
MC
Laboratories CIBA-GEIGY, Biopharmaceutical Research Center, Rueil-Malmaison, France.
Campestrini
J
J
Lecaillon
J B
JB
Godbillon
J
J
D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000097:blood*; D000983:Antipyrine / Q000031:analogs & derivatives* / Q000097:blood; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet
D000894:Anti-Inflammatory Agents, Non-Steroidal; C009077:propyphenazone; D000983:Antipyrine