title: Acid phosphatases in mammalian tissues. Evidence for the existence of a 57 kDa Zn(2+)-dependent acid phosphatase form.
abstract: 1. A comparative study of multiple forms of acid phosphatase (AcPase) in various organs of mammals was carried out. 2. These studies indicated that the high-molecular weight AcPase is preferentially expressed by tissues which undergo cell proliferation such as epithelial tissues; on the contrary, the low-molecular weight enzyme seems to be characteristic of highly differentiated tissues such as nervous, muscle and blood erythrocytes. 3. The existence of a new AcPase activated by Zn2+ ions was observed in all tissues studied with the exception of erythrocytes. 4. The enzyme shows a molecular weight of 57 kDa, is insensitive to NaF, hydrolyzes p-nitro-phenylphosphate and o-c-phenylphosphate; ATP, a-naphthyl-phosphate and beta-glycerolphosphate are also dephosphorylated.
authors.affiliation: Instituto di Anatomia Comparata, Facoltà di Scienze MM.FF.NN., Università di Perugia, Italia.
authors.forename: F
authors.initials: F
authors.lastname: Panara
authors.forename: A
authors.initials: A
authors.lastname: Angiolillo
authors.forename: A
authors.initials: A
authors.lastname: Fagotti
authors.forename: I
authors.initials: I
authors.lastname: Di Rosa
authors.forename: S
authors.initials: S
authors.lastname: Francesca
authors.forename: R
authors.initials: R
authors.lastname: Pascolini
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism*; D000818:Animals; D002413:Cations, Divalent; D002417:Cattle; D002850:Chromatography, Gel; D004789:Enzyme Activation; D006801:Humans; D008322:Mammals; D051381:Rats; D014018:Tissue Distribution; D015032:Zinc / Q000378:metabolism*
chemical_list: D002413:Cations, Divalent; D000135:Acid Phosphatase; D015032:Zinc