title: Accurate histochemical definition of argon-laser-induced tissue necrosis.
abstract: We present a histochemical method using the redox dye nitro blue tetrazolium chloride (NBTC) for the evaluation of argon-laser-induced tissue damage. By this method viable cells are stained blue, devitalized cells not. Our findings suggest that the NBTC staining method is more appropriate for the accurate definition of the necrosis and cell viability after laser treatment than routine histology.
authors.affiliation: Department of Dermatology II, University of Vienna, Austria.
authors.forename: R A
authors.initials: RA
authors.lastname: Neumann
authors.forename: H
authors.initials: H
authors.lastname: Leonhartsberger
authors.forename: F
authors.initials: F
authors.lastname: Pieczkowski
authors.forename: R M
authors.initials: RM
authors.lastname: Knobler
authors.forename: W
authors.initials: W
authors.lastname: Gebhart
mesh_terms: D006391:Hemangioma / Q000473:pathology / Q000601:surgery*; D006801:Humans; D053685:Laser Therapy; D009336:Necrosis; D009580:Nitroblue Tetrazolium; D012867:Skin / Q000473:pathology*; D012878:Skin Neoplasms / Q000473:pathology / Q000601:surgery*; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D009580:Nitroblue Tetrazolium