title: Stable incorporation of genetic material into the chromosome of Rhizobium meliloti 41: construction of an integrative vector system.
abstract: An integrative vector system has been developed from the site-specific recombination elements of temperate phage 16-3. The system can be used for highly efficient stable introduction of genetic material into the chromosome of the symbiotic nitrogen-fixing organism, Rhizobium meliloti 41 (Rm41) at the attB site. Vectors carrying the phage-borne attachment site were constructed, and helper phages providing the site-specific recombination functions in trans were isolated. Other possible applications of the system are discussed.
authors.affiliation: Attila József University, Department of Genetics, Szeged, Hungary.
authors.forename: E
authors.initials: E
authors.lastname: Hermesz
authors.forename: F
authors.initials: F
authors.lastname: Olasz
authors.forename: L
authors.initials: L
authors.lastname: Dorgai
authors.forename: L
authors.initials: L
authors.lastname: Orosz
mesh_terms: D001435:Bacteriophages; D002876:Chromosomes, Bacterial; D003001:Cloning, Molecular; D005822:Genetic Vectors; D010957:Plasmids; D015183:Restriction Mapping; D016962:Sinorhizobium meliloti / Q000235:genetics*