Cellular localization of mRNA for cellular retinoic acid-binding protein II and nuclear retinoic acid receptor-gamma 1 in retinoic acid-treated human skin.
We have previously shown that cellular retinoic acid-binding protein II (CRABP-II), but not cellular retinoic acid-binding protein I (CRABP-I), mRNA expression is markedly induced in human skin by topical retinoic acid. In the present study, we have investigated the pattern of expression of CRABP-II transcripts in 4-d RA-treated human skin by non-radioactive in situ hybridization (n = 5) and Northern analysis (n = 4). RA induced accumulation of CRABP-II transcripts throughout the epidermis, dermal fibroblasts, and endothelial cells as determined by in situ hybridization. In skin treated with vehicle, a faint hybridization signal was observed only in basal layers of the epidermis consistent with low-level expression of CRABP-II mRNA. RA-mediated accumulation of CRABP-II transcripts in skin was also confirmed by Northern analysis. Neither RA nor vehicle induced significant changes in nuclear RA receptor-gamma 1 or keratin 5 gene expression in skin as determined by in situ or Northern hybridization. These results indicate that RA-induced CRABP-II mRNA accumulation is primarily localized to spinous and granular layers in epidermis, and in superficial dermis.
Tavakkol
A
A
Department of Dermatology, University of Michigan Medical Center, Ann Arbor 48109-0528.
Griffiths
C E
CE
Keane
K M
KM
Palmer
R D
RD
Voorhees
J J
JJ
D000287:Administration, Topical; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics*; D002467:Cell Nucleus / Q000648:ultrastructure; D015870:Gene Expression; D006801:Humans; D007633:Keratins / Q000235:genetics; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000032:analysis*; D018168:Receptors, Retinoic Acid; D012867:Skin / Q000187:drug effects / Q000648:ultrastructure*; D014158:Transcription, Genetic; D014212:Tretinoin / Q000008:administration & dosage / Q000494:pharmacology*
D002352:Carrier Proteins; D012333:RNA, Messenger; D018168:Receptors, Retinoic Acid; D014212:Tretinoin; D007633:Keratins