Altered distribution of desmin filaments in hypertrophic cardiomyopathy: an immunohistochemical study.
Hypertrophic cardiomyopathy (HCM) is characterized by myofiber hypertrophy and disarray. Intermediate filaments play an important role in maintaining of normal cell shape. Desmin filaments have been detected in adult cardiomyocytes, and vimentin and keratin filaments in cardiomyocytes during embryonic development. The pattern of arrangement of intermediate filaments in HCM has not been reported. We examined the distribution of intermediate filaments in formalin-fixed tissue sections of the disarrayed myofibers from 10 hearts with HCM using an immunohistochemical technique and antibodies to desmin, vimentin, and high and low molecular weight keratins. The controls consisted of subaortic tissue from surgically explanted hearts of patients with ischemic heart disease. In the ischemic hearts, desmin was detected in the Z bands and intercalated disks. In all HCM cases, three patterns of staining for desmin were noted: (a) individual myocytes showing a parallel arrangement along Z bands; (b) focal myofibers with decreased or complete loss of labeling of Z bands; and (c) individual myocytes with intense granular cytoplasmic staining especially in disarrayed myofibers. No staining for vimentin or keratins was noted in the cardiomyocytes from either the HCM or ischemic cases. The altered arrangement of desmin filaments in the disarrayed cardiac muscle fibers may play a role in the altered contractility that occurs in patients with HCM.
D'Amati
G
G
Department of Pathology, Toronto Hospital and Women's College Hospital, Canada.
Kahn
H J
HJ
Butany
J
J
Silver
M D
MD
D000328:Adult; D000368:Aged; D002312:Cardiomyopathy, Hypertrophic / Q000378:metabolism* / Q000473:pathology; D003893:Desmin / Q000378:metabolism*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D007382:Intermediate Filaments / Q000378:metabolism* / Q000648:ultrastructure; D008297:Male; D008875:Middle Aged; D013194:Staining and Labeling; D014018:Tissue Distribution
D003893:Desmin