Class I restricted CTL recognition of a soluble protein delivered by liposomes containing lipophilic polylysines.
CD8+ cytotoxic lymphocytes recognize peptides derived from endogenous antigens complexed with class I major histocompatibility complex while CD4+ helper cells recognize peptides from exogenous antigens bound to class II MHC molecules. A soluble protein can be introduced into the class I pathway of antigen processing and presentation using an appropriate vehicle to deliver the antigen into the cytosol. Cationic liposomes containing lipophilic polylysine readily form complexes with an anionic, soluble protein ovalbumin. Mouse thymoma EL4 cells incubated with such complexes can be sensitized for killing by OVA-specific CTL effector cells. This method of target sensitization by a soluble antigen is more sensitive than the osmotic loading method previously reported.
Nair
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S
Department of Microbiology, University of Tennessee, Knoxville 37996.
Zhou
X
X
Huang
L
L
Rouse
B T
BT
D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology; D003602:Cytotoxicity, Immunologic; D004337:Drug Carriers; D005260:Female; D015395:Histocompatibility Antigens Class I / Q000276:immunology*; D008081:Liposomes; D051379:Mice; D008810:Mice, Inbred C57BL; D010047:Ovalbumin / Q000276:immunology*; D011107:Polylysine; D013154:Spleen / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*; D013755:Tetradecanoylphorbol Acetate; D014407:Tumor Cells, Cultured
D004337:Drug Carriers; D015395:Histocompatibility Antigens Class I; D008081:Liposomes; D011107:Polylysine; D010047:Ovalbumin; D013755:Tetradecanoylphorbol Acetate