The cDNA structure of the porcine pro-hormone convertase PC2 and the comparative processing by PC1 and PC2 of the N-terminal glycopeptide segment of porcine POMC.
The complete cDNA structure of the porcine (p) pro-protein and pro-hormone convertase PC2 (pPC2) was obtained from a cDNA library of pituitary neurointermediate lobes mRNA. The deduced amino acid sequence revealed that pPC2 exhibits a 99-97% sequence identity to the human, mouse and rat homologues. The 3' end of the 2.1 kb cDNA is the least conserved segment. On Northern blots of pars intermedia poly A+ RNA two transcripts of 3 and 5 kb were detected. Molecular analysis of the N-terminal glycopeptide products of porcine pro-opiomelanocortin (pPOMC) co-expressed with vaccinia virus recombinants of PC1 or PC2, revealed that in cells devoid or containing secretory granules both convertases can cleave pPOMC with PC1 releasing the 1-80, 1-107 and 1-148 glycopeptide fragments, and PC2 cleaving pPOMC directly into pPOMC 1-107.
Seidah
N G
NG
J.A. DeSève Laboratories of Biochemical, Clinical Research Institute of Montreal, Que., Canada.
Fournier
H
H
Boileau
G
G
Benjannet
S
S
Rondeau
N
N
Chrétien
M
M
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D006020:Glycopeptides / Q000378:metabolism; D008954:Models, Biological; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000378:metabolism*; D010902:Pituitary Gland / Q000201:enzymology*; D010907:Pituitary Hormones / Q000378:metabolism; D011333:Pro-Opiomelanocortin / Q000378:metabolism*; D045664:Proprotein Convertase 1; D045706:Proprotein Convertase 2; D043484:Proprotein Convertases; D011499:Protein Processing, Post-Translational; D017422:Sequence Analysis, DNA; D012697:Serine Endopeptidases / Q000235:genetics* / Q000378:metabolism*; D013379:Substrate Specificity; D013552:Swine
D006020:Glycopeptides; D010446:Peptide Fragments; D010907:Pituitary Hormones; C028182:proopiocortin fragments; D011333:Pro-Opiomelanocortin; D043484:Proprotein Convertases; D012697:Serine Endopeptidases; C473656:Pcsk1 protein, mouse; D045664:Proprotein Convertase 1; D045706:Proprotein Convertase 2