Differential human immunodeficiency virus expression in CD4+ cloned lymphocytes: from viral latency to replication.
By using cloning methodology, 13 CD4+, CD8-, CD45RO+, and CD29+ clones, isolated from human immunodeficiency virus (HIV)-negative donors, have been characterized and tested regarding their susceptibility to two strains of HIV type 1 (HIV-1). Infected clones possess integrated provirus. Only six are able to replicate HIV-1, while seven may normally grow without cytopathic effect and without viral replication. These results argue that all CD4+ lymphocyte clones may be infectable but that a heterogeneity exists regarding their abilities to replicate HIV-1.
Chapel
A
A
Laboratoire de Neuropathologie Expérimentale et Neurovirologie, Centre de Recherches du Service de Santé des Armées, Fontenay aux Roses, France.
Bensussan
A
A
Vilmer
E
E
Dormont
D
D
D015703:Antigens, CD / Q000032:analysis; D015496:CD4-Positive T-Lymphocytes / Q000382:microbiology* / Q000473:pathology; D002999:Clone Cells / Q000382:microbiology* / Q000473:pathology; D016655:HIV Core Protein p24 / Q000378:metabolism; D054303:HIV Reverse Transcriptase; D015497:HIV-1 / Q000254:growth & development* / Q000472:pathogenicity; D006801:Humans; D012367:RNA, Viral / Q000032:analysis; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D014775:Virus Activation; D014779:Virus Replication
D015703:Antigens, CD; D016655:HIV Core Protein p24; D012367:RNA, Viral; D054303:HIV Reverse Transcriptase; D012194:RNA-Directed DNA Polymerase