Soluble glycoprotein D blocks herpes simplex virus type 1 infection of rat eyes.
Herpes simplex virus type 1 (HSV-1) ocular infection in rats was blocked by treating the eyes with UV-inactivated virions containing glycoprotein D (gD) prior to ocular challenge. In contrast, rats treated with UV-inactivated virions lacking gD were not protected. A soluble, truncated form of HSV-2 gD (gD-2t) also protected against ocular infection. Treatment with gD-2t not only reduced mortality but also restricted progression of pathology and reduced the amount of viral antigen in the cornea. Host antibody or alpha/beta interferon responses to the gD-2t treatment were not detected. These results are similar to those observed in cell culture (D. C. Johnson, R. L. Burke, and T. Gregory, J. Virol. 64:2569-2576, 1990). The in vivo effect of exogenous gD is consistent with blocking of a cell surface gD receptor or with an inhibitory interaction of gD with virions.
Martin
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Department of Immunology and Microbiology, Wayne State University School of Medicine, Detroit, Michigan 48201.
Montgomery
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Holland
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D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D005260:Female; D007150:Immunohistochemistry; D007372:Interferons / Q000096:biosynthesis; D016849:Keratitis, Herpetic / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*; D051381:Rats; D011916:Rats, Inbred F344; D011991:Receptors, Virus / Q000502:physiology; D018139:Simplexvirus / Q000276:immunology / Q000472:pathogenicity* / Q000528:radiation effects; D012995:Solubility; D016019:Survival Analysis; D014466:Ultraviolet Rays; D014759:Viral Envelope Proteins / Q000494:pharmacology*; D014774:Virulence
D000914:Antibodies, Viral; D011991:Receptors, Virus; D014759:Viral Envelope Proteins; C039447:glycoprotein D, Human herpesvirus 1; D007372:Interferons