Delineation of structural domains involved in the subtype specificity of tachykinin receptors through chimeric formation of substance P/substance K receptors.
The mammalian tachykinin receptors belong to the family of G protein-coupled receptors and consist of the substance P, substance K and neuromedin K receptors (SPR, SKR and NKR). We constructed 14 chimeric receptors in which seven transmembrane segments were sequentially exchanged between the rat SPR and SKR and examined the subtype specificity of the chimeric receptors by radioligand binding and inositol phosphate measurements after transfection into COS cells. All chimeric receptors showed maximum responses in agonist-induced inositol phosphate stimulation. Detailed analysis of five receptors with agonist selectivity similar to SPR indicated that the selectivity is mainly determined by the region extending from transmembrane segment II to the second extracellular loop together with a minor contribution of the extracellular N-terminal portion. This conclusion was more directly confirmed by an additional chimeric formation in which the introduction of the above middle portion of SPR into the corresponding region of SKR conferred a high affinity binding to substance P. The tachykinin receptors can thus be divided into two functional domains: the region covering transmembrane segments V-VII and responsible for fundamental recognition of the common tachykinin sequence; and its preceding portion involved in evoking subtype specificity by interacting with the divergent sequences of the peptides.
Yokota
Y
Y
Institute for Immunology, Kyoto University Faculty of Medicine, Japan.
Akazawa
C
C
Ohkubo
H
H
Nakanishi
S
S
D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000502:physiology; D004247:DNA / Q000235:genetics; D007457:Iodine Radioisotopes; D008954:Models, Biological; D015288:Neurokinin A / Q000378:metabolism*; D010716:Phosphatidylinositols / Q000378:metabolism; D011869:Radioligand Assay; D051381:Rats; D018040:Receptors, Neurokinin-1; D018041:Receptors, Neurokinin-2; D017981:Receptors, Neurotransmitter / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D015183:Restriction Mapping; D015398:Signal Transduction; D013373:Substance P / Q000378:metabolism*; D013379:Substrate Specificity; D014162:Transfection
D007457:Iodine Radioisotopes; D010716:Phosphatidylinositols; D018040:Receptors, Neurokinin-1; D018041:Receptors, Neurokinin-2; D017981:Receptors, Neurotransmitter; D011993:Recombinant Fusion Proteins; D013373:Substance P; D015288:Neurokinin A; D004247:DNA