Effects of human, rat and porcine galanins on cardiac vagal action and blood pressure in the anaesthetised cat.
Galanin (GAL) is distributed in sympathetic nerves in the cat, and exogenous GAL inhibits cardiac vagal action and lowers blood pressure in this species. This study on anaesthetised cats compares the effects on cardiac vagal action and blood pressure of human, rat and porcine GAL. Human GAL has only recently been sequenced. It is of particular interest as it is not C-terminally amidated, unlike porcine and rat GAL. Many regulatory peptides require a C-terminal amide group for their action. However, human GAL showed similar biological activity to the other (amidated) GALs here. Omission of a single amino acid (Ser6) from rat GAL significantly attenuated both cardiovascular actions studied here.
Ulman
L G
LG
Prince of Wales Medical Research Institute, Prince of Wales Hospital, Syndey, Australia.
Evans
H F
HF
Iismaa
T P
TP
Potter
E K
EK
McCloskey
D I
DI
Shine
J
J
D000595:Amino Acid Sequence; D000818:Animals; D001794:Blood Pressure / Q000187:drug effects*; D002415:Cats; D003864:Depression, Chemical; D019004:Galanin; D006329:Heart Conduction System / Q000187:drug effects*; D006339:Heart Rate / Q000187:drug effects; D006801:Humans; D008969:Molecular Sequence Data; D010455:Peptides / Q000235:genetics / Q000494:pharmacology*; D051381:Rats / Q000235:genetics / Q000378:metabolism; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D013552:Swine / Q000235:genetics / Q000378:metabolism; D014630:Vagus Nerve / Q000187:drug effects* / Q000502:physiology
D010455:Peptides; D019004:Galanin