title: Influence of the estrous cycle at gamma-ray exposure on radiation-induced mammary tumorigenesis in virgin rats.
abstract: Wistar rats received whole body irradiation with 260 cGy gamma-rays at 10 a.m. of individual phases of their estrous cycle and then had diethylstilbestrol pellets implanted for 1 year. When the radiation was given during di-estrus II, the highest incidence (73.3%) of mammary tumorigenesis was observed, and mean latency until the first tumor appearance was 8.5 +/- 0.7 months. Also, rats irradiated on estrus had significantly lower incidence (35.3%) of mammary tumors than those irradiated on pro-estrus and di-estrus II, but there was no significant difference in latency period. Iball's indices of total mammary tumors, fibroadenoma plus adenocarcinoma, were 14.3 +/- 0.9, 28.8 +/- 2.1, 23.3 +/- 0.8 and 12.2 +/- 0.2 in rats irradiated during di-estrus I, di-estrus II, pro-estrus and estrus respectively. The percentage of adenocarcinomas was comparatively uniform (25.0 to 34.8%) throughout the various phases of the estrous cycles. Also, Iball's indices calculated from adenocarcinoma indicated no significant differences between all groups. From our results, the highest incidence of mammary tumors arose in rats after irradiation at di-estrus II with minimum level of prolactin in serum. We discuss different mechanisms of radiation-induced and chemical-carcinogen-induced tumorigenesis of mammary glands.
authors.affiliation: Division of Chemical Pharmacology, National Institute of Radiological Sciences, Chiba-shi, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Inano
authors.forename: H
authors.initials: H
authors.lastname: Ishii-Ohba
authors.forename: K
authors.initials: K
authors.lastname: Suzuki
authors.forename: H
authors.initials: H
authors.lastname: Yamanouchi
mesh_terms: D000230:Adenocarcinoma / Q000209:etiology; D000818:Animals; D004054:Diethylstilbestrol / Q000633:toxicity; D004971:Estrus; D005260:Female; D005720:Gamma Rays; D008325:Mammary Neoplasms, Experimental / Q000209:etiology*; D009381:Neoplasms, Radiation-Induced / Q000209:etiology*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D004054:Diethylstilbestrol