[Relationship between endothelial cells and extracellular matrix: investigation using the model of angiogenesis in vitro].
Using model of angiogenesis in vitro, the relationship between endothelial cells and extracellular matrix was studied. Endothelial cells of bovine brain microvascular vessels (BBECs), carotid artery (BCECs) and aorta (BAECs) were cultured on type I collagen gel and Matrigel. BBECs make tubular structures and BCECs and BAECs grow and make confluent monolayer on type I collagen gel. BCECs and BAECs make tubular structures when second layer of collagen gel was overlaid. BBECs, BCECs and BAECs rapidly make tubular structures on Matrigel. These morphological changes were not affected by basic fibroblast growth factor. The effect of extracellular matrices on the cell kinetics and morphology of cultured bovine carotid artery endothelial cells was studied. Endothelial cells show a cobble stone appearance on plastic and type I collagen gel. They proliferate and make capillary-like structures on reconstituted gels composed of type I collagen and basement membrane substrata. And that endothelial cells forming capillary-like structures were increased with the increase in the concentration of basement membrane substrata. Transmission electromicroscopic examination study revealed endothelial cells forming capillary-like structures have junctional complexes on type I collagen and its mixture with basement membrane substrata. But endothelial cells on basement membrane substrata have no junctional complexes. These results suggest that BBECs have more potent angiogenic ability than BCECs and BAECs. And that proliferation and morphogenesis of endothelial cells are regulated by extracellular matrices.
Kaneko
T
T
Department of Neurosurgery, Faculty of Medicine, Kyoto University, Japan.
D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000473:pathology* / Q000648:ultrastructure; D005109:Extracellular Matrix / Q000473:pathology* / Q000648:ultrastructure; D008854:Microscopy, Electron; D008955:Models, Cardiovascular; D009389:Neovascularization, Pathologic / Q000473:pathology*