Structures of the O chains from lipopolysaccharides of Campylobacter jejuni serotypes O:23 and O:36.
Lipopolysaccharides of C. jejuni serotypes O:23 and O:36 have been shown to contain structurally variable O polysaccharide chains with repeating units of four closely-related types: ----3)-beta-D-GlcpNAc-(1----3)-alpha-D-Galp-(1----2)-6d-alpha-D-alt-H epp-(1---- , ----3)-beta-D-GlcpNAc-(1----3)-alpha-D-Galp-(1----2)-6d-3-Me-alpha-D-alt -Hepp- (1----, ----3)-beta-D-GlcpNAc-(1----3)-alpha-D-Galp-(1----2)-D-glycero-alpha-D-a lt-Hepp - (1----, and ----3)-beta-D-GlcpNAc-(1----3)-alpha-D-Galp-(1----2)-3-Me-D-glycero-alph a-D-alt - Hepp-(1----. Structural methods included 1H- and 13C-NMR spectroscopy, methylation linkage analysis, fast atom bombardment mass spectrometry of methylated glycans, and selective fragmentations by the Smith degradation and N-deacetylation-nitrous acid deamination.
Aspinall
G O
GO
Department of Chemistry, York University, Toronto, Ontario, Canada.
McDonald
A G
AG
Pang
H
H
D016123:Campylobacter jejuni / Q000737:chemistry* / Q000145:classification; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002849:Chromatography, Gas; D007202:Indicators and Reagents; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008745:Methylation; D008969:Molecular Sequence Data; D015394:Molecular Structure; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000302:isolation & purification; D016339:Spectrometry, Mass, Fast Atom Bombardment
D007202:Indicators and Reagents; D008070:Lipopolysaccharides; D011135:Polysaccharides, Bacterial