title: Cloning, sequencing, and expression of the Escherichia coli peptide methionine sulfoxide reductase gene.
abstract: The gene encoding peptide methionine sulfoxide reductase was cloned from an Escherichia coli genomic library using an oligonucleotide probe based on the amino-terminal sequence of the protein. The nucleotide sequence revealed that the gene codes for a polypeptide of 212 amino acid residues with a calculated molecular weight of 23,314. The protein has been overexpressed in E. coli and is present as a soluble active species.
authors.affiliation: Roche Institute of Molecular Biology, Roche Research Center, Nutley, New Jersey 07110.
authors.forename: M A
authors.initials: MA
authors.lastname: Rahman
authors.forename: H
authors.initials: H
authors.lastname: Nelson
authors.forename: H
authors.initials: H
authors.lastname: Weissbach
authors.forename: N
authors.initials: N
authors.lastname: Brot
mesh_terms: D000595:Amino Acid Sequence; D010582:Bacteriophage lambda / Q000235:genetics; D001483:Base Sequence; D003001:Cloning, Molecular; D015342:DNA Probes; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics*; D005798:Genes, Bacterial; D015698:Genomic Library; D007700:Kinetics; D051150:Methionine Sulfoxide Reductases; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D010088:Oxidoreductases / Q000235:genetics* / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism
chemical_list: D015342:DNA Probes; D015345:Oligonucleotide Probes; D011994:Recombinant Proteins; D010088:Oxidoreductases; D051150:Methionine Sulfoxide Reductases; C027219:methionine sulfoxide reductase