Establishment of a human hepatoblastoma model in athymic nude mice. Producibility of fatty acid-binding protein, alpha-fetoprotein, and alpha 1-acid glycoprotein.
An athymic nude mouse model of human hepatoblastoma, designated HBL-2, was established. HBL-2 produced alpha-fetoprotein (AFP) and the serum level in tumor-bearing mouse was roughly proportional to the tumor weight. Reactivity of AFP to Concanavalin A and Lens culinaris lectins indicated that it contained predominantly fucosylated, non-bisecting N-acetylglucosamine as a sugar moiety. Alpha 1-acid glycoprotein (AAG) was also found in both HBL-2 tumor extract and sera of tumor-bearing mice. The serum level, however, was not proportional to either the tumor weight or the value of the tumor extract. In addition, fatty acid-binding protein (FABP) was detected in approximately 40% of tumor cells and in tumor extract with polyclonal antibody against liver FABP. Thus, HBL-2 is an unique model of human hepatoblastoma, in that it produces FABP in addition to AAG and AFP.
Suzuki
T
T
Department of Pathology, Fukushima Medical College, Japan.
Watanabe
K
K
Hirota
M
M
Iwafuchi
M
M
Ono
T
T
Aoyagi
Y
Y
Ito
S
S
D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000737:chemistry / Q000473:pathology*; D002352:Carrier Proteins / Q000032:analysis*; D004195:Disease Models, Animal; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D006801:Humans; D007150:Immunohistochemistry; D007223:Infant; D008113:Liver Neoplasms / Q000737:chemistry / Q000473:pathology*; D008297:Male; D051379:Mice; D008819:Mice, Nude; D009363:Neoplasm Proteins; D009368:Neoplasm Transplantation; D009419:Nerve Tissue Proteins; D009961:Orosomucoid / Q000032:analysis*; D025521:Tumor Suppressor Proteins; D000509:alpha-Fetoproteins / Q000032:analysis*
D002352:Carrier Proteins; C107608:FABP7 protein, human; C082667:Fabp5 protein, mouse; C086936:Fabp7 protein, mouse; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D009961:Orosomucoid; D025521:Tumor Suppressor Proteins; D000509:alpha-Fetoproteins