Soybean trypsin inhibitor and beta-amylase induce alveolar macrophages to release nitrogen oxides.
Rat alveolar macrophages incubated with soybean trypsin inhibitor and beta-amylase produced nitrite in a dose- and time-dependent manner. This production depends on the presence of L-arginine (L-arg) in the culture medium. The precursor of this nitrite was demonstrated as being nitric oxide by bleaching ferredoxin at 410 nm when added to the culture medium. NG-Monomethyl-L-arginine and the tetrahydrobiopterin biosynthesis inhibitor 2,4-diamino-6-hydroxypyrimidine inhibited the release of nitrite in a dose-dependent manner. Dexamethasone was able to modulate this release. These data indicate that alveolar macrophages are capable of secreting L-arg-derived nitrogen oxides when stimulated with certain alimentary proteins.
Jorens
P G
PG
Department of Respiratory Medicine, University of Antwerp, Belgium.
Van Overveld
F J
FJ
Bult
H
H
Vermeire
P A
PA
Herman
A G
AG
D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives / Q000494:pharmacology; D002478:Cells, Cultured / Q000187:drug effects; D003907:Dexamethasone / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D007042:Hypoxanthines / Q000494:pharmacology; D008033:Limulus Test; D016676:Macrophages, Alveolar / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D009573:Nitrites / Q000378:metabolism; D009589:Nitrogen Oxides / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors; D014361:Trypsin Inhibitors / Q000494:pharmacology*; D001614:beta-Amylase / Q000494:pharmacology*; D019323:omega-N-Methylarginine
D007042:Hypoxanthines; D009573:Nitrites; D009589:Nitrogen Oxides; D014361:Trypsin Inhibitors; D019323:omega-N-Methylarginine; D003907:Dexamethasone; D001120:Arginine; D001614:beta-Amylase; C017202:2,4-diaminohypoxanthine