Maturation of the hypothalamo-pituitary-gonadal axis of male Syrian hamsters.
Light-microscope immunocytochemistry (ICC) was used to investigate postnatal changes in the morphology of LHRH neurons in the brains of male Syrian hamsters and to relate these changes to more overt maturational developments within the hypothalamo-pituitary-gonadal axis. The animals were maintained under long-day photoperiods (14L:10D), and groups of 6-7 were killed at 10-day intervals from Day 15 to Day 65. Their brains were fixed with 4% paraformaldehyde, sectioned sagittally with a vibratome (75 microns), and processed for ICC using monoclonal LHRH antibody HU4H. Throughout the study period, the hamsters showed a progressive increase in plasma gonadotropin levels, closely followed by an increase in testicular weight and plasma testosterone levels. Histology of the testes revealed that spermatogenesis was already qualitatively completed by Day 35 and quantitative aspects were established by Day 45. Within the brain, LHRH neuronal perikarya were distributed primarily in the medial septal-preoptic area and the diagonal band of Broca; morphologically, these immunopositive neurons were either monopolar or bipolar. The total number of LHRH neurons detected in the areas examined was approximately 440 throughout the developmental period, and the relative proportions of monopolar and bipolar subtypes (86% and 14%, respectively) remained unchanged. In contrast, the area of the perikarya, as determined by autoimage analysis, showed a highly significant age-related increase, both for the monopolar and bipolar neurons. It is suggested that these developmental changes in the LHRH neurons reflect an increase in LHRH synthesis and may, therefore, provide a neuroendocrine trigger for the onset of puberty.
Urbanski
H F
HF
Division of Neuroscience, Oregon Regional Primate Research Center, Beaverton 97006.
Doan
A
A
Pierce
M
M
Fahrenbach
W H
WH
Collins
P M
PM
D000818:Animals; D001921:Brain / Q000166:cytology / Q000254:growth & development; D006224:Cricetinae; D007987:Gonadotropin-Releasing Hormone / Q000378:metabolism; D007031:Hypothalamus / Q000254:growth & development*; D007150:Immunohistochemistry; D008297:Male; D008647:Mesocricetus / Q000254:growth & development*; D009474:Neurons / Q000166:cytology; D009929:Organ Size; D010902:Pituitary Gland / Q000254:growth & development*; D012741:Sexual Maturation; D013737:Testis / Q000166:cytology / Q000254:growth & development*
D007987:Gonadotropin-Releasing Hormone