Involvement of cyclic adenosine 3',5'-monophosphate in methylation during 1-methyladenine production by starfish ovarian follicle cells.
Resumption of meiosis in starfish oocytes is induced by 1-methyladenine (1-MeAde) produced by ovarian follicle cells under the influence of a gonad-stimulating substance (GSS). With respect to 1-MeAde production, the effect of GSS on follicle cells results in the receptor-mediated formation of cyclic AMP (cAMP). It has also been reported that methylation is involved in 1-MeAde production by GSS. This study was undertaken to determine whether cAMP is the agent responsible for mediating methylation in 1-MeAde biosynthesis by isolated follicle cells of the starfish Asterina pectinifera. Methionine and selenomethionine enhanced 1-MeAde production by GSS in follicle cells. These stimulatory effects were dependent on the GSS concentration. Production of 1-MeAde by GSS was inhibited by ethionine and selenoethionine, competitive inhibitors of methionine. Like GSS, 1-MeAde production induced by concanavalin A, trypsin, and 3-isobutyl-1-methylxanthine (IBMX), which stimulated cAMP accumulation in follicle cells, was influenced by methionine and its related compounds. In contrast, although 1-methyladenosine (1-MeAde-R) induced 1-MeAde production by follicle cells without increasing cAMP levels, methionine and its related compounds had no effect. Use of [methyl-14C]methionine showed that a radiolabel was incorporated into 1-MeAde during incubation with GSS and IBMX, but not with 1-MeAde-R. These results strongly suggest that cAMP plays an important role in the process of methylation during 1-MeAde biosynthesis induced by GSS.
Mita
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Department of Biochemistry, Teikyo University School of Medicine, Tokyo, Japan.
D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D000225:Adenine / Q000031:analogs & derivatives* / Q000096:biosynthesis; D000241:Adenosine / Q000031:analogs & derivatives / Q000494:pharmacology; D000818:Animals; D003208:Concanavalin A / Q000494:pharmacology; D000242:Cyclic AMP / Q000378:metabolism*; D005260:Female; D008715:Methionine / Q000494:pharmacology*; D008716:Methionine Adenosyltransferase / Q000378:metabolism; D008745:Methylation; D006080:Ovarian Follicle / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D013215:Starfish / Q000378:metabolism*; D014357:Trypsin / Q000494:pharmacology
D003208:Concanavalin A; C002230:1-methyladenosine; C008407:1-methyladenine; D008715:Methionine; D000242:Cyclic AMP; D008716:Methionine Adenosyltransferase; D014357:Trypsin; D000225:Adenine; D000241:Adenosine; D015056:1-Methyl-3-isobutylxanthine