Monitor peptide gene expression is increased by exogenous CCK in the rat pancreas and in a rat pancreatic acinar cell line (AR4-2J).
Monitor peptide (CCK-releasing peptide) mRNA increased on the administration of CCK in rat pancreas and the AR4-2J pancreatic cell line. Subcutaneous injection of CCK into rats at 8 h intervals increased the level of monitor peptide mRNA in the pancreas. Concomitant injection of CCK antagonist CR-1409 strongly decreased it. The monitor peptide mRNA was also increased by CCK in AR4-2J cells and was decreased by the antagonist. These findings suggest that the plasma CCK induced by prolonged intake of a high protein diet may be responsible for the adaptative increase in the monitor peptide as well as exocrine proteases in the pancreas.
Tsuzuki
S
S
Department of Food Science and Technology, Faculty of Agriculture, Kyoto University, Japan.
Kondo
A
A
Fushiki
T
T
Sugimoto
E
E
D000681:Amylases / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002460:Cell Line; D002766:Cholecystokinin / Q000037:antagonists & inhibitors / Q000502:physiology*; D002919:Chymotrypsinogen / Q000235:genetics; D005786:Gene Expression Regulation; D006133:Growth Substances; D036341:Intercellular Signaling Peptides and Proteins; D008297:Male; D008969:Molecular Sequence Data; D010179:Pancreas / Q000166:cytology / Q000378:metabolism*; D011377:Proglumide / Q000031:analogs & derivatives / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D014359:Trypsin Inhibitor, Kazal Pancreatic / Q000235:genetics*
D006133:Growth Substances; D036341:Intercellular Signaling Peptides and Proteins; D012333:RNA, Messenger; C504705:Spink1 protein, rat; D014359:Trypsin Inhibitor, Kazal Pancreatic; D002766:Cholecystokinin; D002919:Chymotrypsinogen; D000681:Amylases; D011377:Proglumide; C048181:lorglumide