Cell cycle regulation (G1) by autocrine interferon and dissociation between autocrine interferon and 2',5'-oligoadenylate synthetase expression.
In tertiary MEF undergoing cell cycle progression, autocrine interferon (IFN) is released and constitutive levels of 2',5'-oligoadenylate (2-5A) synthetase activity, low through the cell cycle, surge into a peak within S phase. Treatment of MEF with the autocrine IFN they produce elicits a 2-5A synthetase response from cells positioned in G0 but not from cells in G1 and from cells in S phase. Neutralization of the autocrine IFN by antibody shortens the length of G1 leaving unaltered the kinetics of progression through S and G2 and has no effect on the S phase-linked expression of 2-5A synthetase activity. The growth controlling effect of the autocrine IFN has been mapped to the second part of G1.
Wells
V
V
Microbiology Department, United Medical School of Guy's Hospital, London, UK.
Mallucci
L
L
D015088:2',5'-Oligoadenylate Synthetase / Q000096:biosynthesis*; D000818:Animals; D000911:Antibodies, Monoclonal; D002460:Cell Line; D016193:G1 Phase / Q000502:physiology*; D007372:Interferons / Q000276:immunology / Q000502:physiology*; D016196:S Phase / Q000502:physiology
D000911:Antibodies, Monoclonal; D007372:Interferons; D015088:2',5'-Oligoadenylate Synthetase