Effect of recombinant human granulocyte colony-stimulating factor (rhG-CSF) on circulating platelets.
The effect on platelets of rhG-CSF was studied in 20 healthy volunteers with the thrombometer, a specially developed device which is described in detail. Additionally, conventional aggregation tests were performed. Low doses of rhG-CSF enhance functional platelet activity, as shown by significant acceleration of the occlusion of the thrombometer channel. Similar results were found in conventional aggregation tests utilizing collagen for induction. At G-CSF concentrations of 0.1 and 1.0 ng/ml the time to response was significantly accelerated and the maximum response was observed in a higher proportion of platelets. However, the second phase of aggregation induced by epinephrine was significantly inhibited by 1.0 ng/ml G-CSF. The activation of platelets may be beneficial in thrombocytopenia, but it may also increase platelet turnover and platelet loss. Further investigation is needed to clarify the mechanism by which G-CSF exerts its effects on platelets.
Avenarius
H J
HJ
Zentrum Innere Medizin, Abteilung Hämatologie, Medizinische Hochschule Hannover, Federal Republic of Germany.
Freund
M
M
Deinhardt
J
J
Poliwoda
H
H
D000328:Adult; D001775:Blood Circulation; D001792:Blood Platelets / Q000187:drug effects*; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology*; D006801:Humans; D008297:Male; D009994:Osmolar Concentration; D010973:Platelet Adhesiveness / Q000187:drug effects; D010974:Platelet Aggregation / Q000187:drug effects; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology; D016188:Receptors, Granulocyte Colony-Stimulating Factor; D011994:Recombinant Proteins
D010975:Platelet Aggregation Inhibitors; D016188:Receptors, Granulocyte Colony-Stimulating Factor; D011994:Recombinant Proteins; D016179:Granulocyte Colony-Stimulating Factor