Esterase electrophoresis compared with biotyping for epidemiological typing of Acinetobacter baumannii strains.
Forty-nine Acinetobacter baumannii strains belonging to three biotypes and isolated from four hospitals were differentiated by electrophoretic typing of their esterases. Six main kinds of esterases were distinguished by their spectra of hydrolytic activity toward seven synthetic substrates. The electrophoretic variations of these enzymes were used to define ten zymotypes among the three biotypes. Esterase electrophoresis appeared to be more sensitive than biotyping, and could represent an additional marker for epidemiological analysis.
Pouëdras
P
P
Laboratoire de Bactériologie-Virologie, Hôpital Universitaire Pontchaillou, Rennes, France.
Gras
S
S
Sire
J M
JM
Mesnard
R
R
Donnio
P Y
PY
Picard
B
B
Avril
J L
JL
D000150:Acinetobacter / Q000145:classification* / Q000201:enzymology* / Q000302:isolation & purification; D000151:Acinetobacter Infections / Q000382:microbiology; D015373:Bacterial Typing Techniques; D003428:Cross Infection / Q000382:microbiology; D004591:Electrophoresis, Polyacrylamide Gel; D004950:Esterases / Q000302:isolation & purification*; D005069:Evaluation Studies as Topic; D006801:Humans; D016036:Seroepidemiologic Studies; D013045:Species Specificity
D004950:Esterases