Surface-reactive biomaterials in osteoblast cultures: an ultrastructural study.
The tissue/biomaterial interface reactions of three biomaterials selected as candidates for hard tissue replacement were studied at the electron microscopical level after incubation with enzymatically isolated rat bone cells. An electron-dense layer was routinely observed between hydroxyapatite, coral, cytodex polymer and the neighbouring cells. This layer was visible before bone formation occurred, and was collagen free. The ultrastructural features revealed a needle-shaped filamentous layer continuous with coral material, whereas hydroxyapatite or cytodex/tissue interface was granular in appearance. These different structures may indicate reactive surfaces, depending on the composition of the substrate.
Sautier
J M
JM
Laboratoire de Biologie-Odontologie, Université Paris VII, France.
Nefussi
J R
JR
Forest
N
N
D000818:Animals; D001672:Biocompatible Materials; D002478:Cells, Cultured; D003911:Dextrans; D017886:Durapatite; D006882:Hydroxyapatites; D008422:Materials Testing; D008854:Microscopy, Electron; D010006:Osteoblasts / Q000166:cytology*; D013499:Surface Properties
D001672:Biocompatible Materials; D003911:Dextrans; D006882:Hydroxyapatites; C052839:Cytodex; D017886:Durapatite