Rat bone marrow erythroid cell fractionation by counter current distribution in non-charge-sensitive two-phase systems.
Counter-current distribution in non charge-sensitive aqueous poly(ethylene glycol)-dextran two phase systems allows the fractionation of rat bone marrow cells into two broad cell subpopulations with different distribution coefficients in a relatively short time. Morphological identification and enzymatic studies suggest that erythroid cells are mainly present in the subpopulation with the higher distribution coefficient. The distribution coefficient and, therefore, surface hydrophobicity of these cells, apparently increase in parallel with an increase in their degree of differentiation and maturation.
Garcia-Pérez
A I
AI
Departamento de Bioquímica y Biología Molecular, Universidad de Alcalá, Alcalá de Henares, Madrid, Spain.
Sancho
P
P
Mendieta
J
J
Luque
J
J
D000818:Animals; D001731:Bisphosphoglycerate Mutase / Q000032:analysis; D001854:Bone Marrow Cells; D002454:Cell Differentiation; D002469:Cell Separation / Q000379:methods*; D003377:Countercurrent Distribution; D003911:Dextrans; D004912:Erythrocytes; D008297:Male; D011092:Polyethylene Glycols; D011770:Pyruvate Kinase / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D012156:Reticulocytes
D003911:Dextrans; D011092:Polyethylene Glycols; D011770:Pyruvate Kinase; D001731:Bisphosphoglycerate Mutase