Construction of a chromosome-enriched HpaII library from flow-sorted wheat chromosomes.
We report here the first successful generation of a chromosome-enriched library from flow sorted plant chromosomes. Chromosomes with a characteristic DNA content (a peak) were sorted from a synchronized cell culture (TaKB1, derived from Triticum aestivum). A HpaII library was constructed from the sorted chromosomes and half of the cloned DNA sequences analysed are unique or low copy. Approximately half of these sequences when used as probes detect sequences on wheat chromosome 4A. The generation and analysis of the chromosome library is described in detail and the prospects of using flow-sorted plant chromosomes discussed.
Wang
M L
ML
Cambridge Laboratory, JI Centre for Plant Science Research, Norwich, UK.
Leitch
A R
AR
Schwarzacher
T
T
Heslop-Harrison
J S
JS
Moore
G
G
D002478:Cells, Cultured; D002875:Chromosomes / Q000378:metabolism*; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D015342:DNA Probes / Q000235:genetics; D019003:Deoxyribonuclease HpaII; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism*; D005434:Flow Cytometry; D015723:Gene Library; D014908:Triticum / Q000235:genetics*
D015342:DNA Probes; D004247:DNA; D019003:Deoxyribonuclease HpaII; D015252:Deoxyribonucleases, Type II Site-Specific