Induction of angiogenesis by growth factors: relevance to pancreatic islet transplantation.
Biodegradable pellets releasing 20 ng/day of endothelial cell growth factor alpha (alpha ECGF) or a- or b-fibroblast growth factor (FGF) and 90 micrograms/day of heparin were implanted beneath the renal capsule in rats and dogs and the muscularis/serosal border of the pyloric stomach in dogs to test for angiogenesis in a potential pancreatic islet transplant site. These factors were also tested in vitro to determine whether the capillary bed of the isolated islet could be preserved. alpha ECGF was superior to a- or bFGF in promoting endothelial cell growth and capillary formation in isolated islets. Both a- or bFGF and alpha ECGF induced the development of a dense capillary bed in the dog stomach, whereas in the kidney site alpha ECGF was more effective in the rat than was a- or bFGF. Priming the isolated islet as well as the transplant site prior to islet transplantation resulted in islet blood flow being established within 3 days in contrast to 7-14 days in controls.
Stagner
J I
JI
Veterans Administration Medical Center, Louisville, KY.
Samols
E
E
D000818:Animals; D002196:Capillaries / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D004176:Dipyridamole / Q000494:pharmacology; D004285:Dogs; D016228:Endothelial Growth Factors / Q000494:pharmacology*; D016220:Fibroblast Growth Factor 1 / Q000494:pharmacology*; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D007515:Islets of Langerhans / Q000098:blood supply*; D016381:Islets of Langerhans Transplantation / Q000502:physiology*; D009389:Neovascularization, Pathologic; D051381:Rats; D012039:Regional Blood Flow / Q000187:drug effects; D014183:Transplantation, Heterologous
D016228:Endothelial Growth Factors; D016222:Fibroblast Growth Factor 2; D016220:Fibroblast Growth Factor 1; D004176:Dipyridamole