Colocalization of nitric oxide synthase and NADPH-diaphorase in cultured myenteric neurones.
Nitric oxide synthase immunoreactivity and NADPH-diaphorase activity were examined in explant culture preparations of the myenteric plexus from beneath the taenia coli of the guinea-pig caecum. Nitric oxide synthase immunoreactive neurones formed approximately one third of the total neuronal population. NADPH-diaphorase positive neurones, demonstrated histochemically, constituted a similar proportion of the total number of neurones. Immunocytochemistry and NADPH-diaphorase histochemistry performed on the same preparations revealed that all nitric oxide synthase immunoreactive neurones expressed NADPH-diaphorase activity. This histochemical evidence is consistent with the view that nitric oxide may act as a regulatory agent in the guinea-pig caecum.
Saffrey
M J
MJ
Department of Anatomy and Developmental Biology, University College, London, UK.
Hassall
C J
CJ
Hoyle
C H
CH
Belai
A
A
Moss
J
J
Schmidt
H H
HH
Förstermann
U
U
Murad
F
F
Burnstock
G
G
D000594:Amino Acid Oxidoreductases / Q000032:analysis*; D000818:Animals; D000831:Animals, Newborn; D003106:Colon / Q000294:innervation*; D006168:Guinea Pigs; D007150:Immunohistochemistry; D009197:Myenteric Plexus / Q000166:cytology / Q000201:enzymology*; D009252:NADPH Dehydrogenase / Q000032:analysis*; D009474:Neurons / Q000166:cytology / Q000201:enzymology*; D019001:Nitric Oxide Synthase; D009924:Organ Culture Techniques
D019001:Nitric Oxide Synthase; D000594:Amino Acid Oxidoreductases; D009252:NADPH Dehydrogenase