The effects of poly(I).poly(C12U) and interferon on the multiplication of a mammalian type C retrovirus in human cells.
Poly(I).poly(C12U) or interferon treatment inhibited multiplication of the xenotropic baboon type C endogenous retrovirus M7 in chronically infected human AV3-M7 cells, as determined by a reverse transcriptase (RT) assay and electron microscopy. Furthermore, this polynucleotide induced 2'5' oligoadenylate (2'5'A) synthetase activity. In contrast to interferon (IFN), poly(I).poly(C12U) did not give rise to the appearance of a trapping phenomenon observable by electron microscopy. When AV3-M7 cells were treated simultaneously with poly(I).poly(C12U) and anti-IFN-beta/alpha antibodies, the induction of 2'5'A synthetase was abolished without any alteration of the inhibitory effect of RT activity. Taken together, these results suggest that different mechanisms are used by poly(I).poly(C12U) and IFN in blocking type C retrovirus multiplication.
Chelbi-Alix
M K
MK
U245 INSERM, Paris, France.
Belforte
B
B
Saal
F
F
Lasneret
J
J
Peries
J
J
Thang
M N
MN
D015088:2',5'-Oligoadenylate Synthetase / Q000096:biosynthesis; D000818:Animals; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004790:Enzyme Induction; D006801:Humans; D016898:Interferon-alpha / Q000276:immunology / Q000494:pharmacology; D016899:Interferon-beta / Q000276:immunology / Q000494:pharmacology; D007371:Interferon-gamma / Q000494:pharmacology; D007372:Interferons / Q000276:immunology / Q000494:pharmacology*; D010215:Papio / Q000382:microbiology; D011070:Poly I-C / Q000494:pharmacology*; D011072:Poly U / Q000494:pharmacology*; D012194:RNA-Directed DNA Polymerase / Q000096:biosynthesis; D015301:Retroviruses, Simian / Q000187:drug effects* / Q000254:growth & development* / Q000648:ultrastructure; D014771:Virion / Q000187:drug effects; D014779:Virus Replication / Q000187:drug effects*
D016898:Interferon-alpha; D011072:Poly U; D016899:Interferon-beta; D007371:Interferon-gamma; D007372:Interferons; C047490:poly(I).poly(c12,U); D012194:RNA-Directed DNA Polymerase; D015088:2',5'-Oligoadenylate Synthetase; D011070:Poly I-C