Alternative secondary structures in the phage G4 origin of the complementary DNA strand synthesis: effects of NaCl concentration on the bleomycin-DNA interaction.
The effects of NaCl concentration on bleomycin-induced cleavages of single-strand and double-strand DNA fragments containing the phage G4 origin of complementary DNA strand synthesis were investigated. It was found that bleomycin could be used as a reagent to analyze secondary and tertiary structures and subtle changes of DNA structures. The effects of NaCl concentration on cleavages of single-stranded DNA were distinct at every target site, indicating that the diversity of topolotical properties of DNA might change the selectivity of the bleomycin-induced DNA cleavage. These results showed alternative secondary structures within and close to the G4 origin of complementary DNA strand synthesis.
Ueda
K
K
Department of Agricultural Chemistry, Kyoto University, Japan.
Hiasa
H
H
Takebe
S
S
Sakai
H
H
Komano
T
T
D001435:Bacteriophages / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D001761:Bleomycin / Q000494:pharmacology*; D004247:DNA / Q000032:analysis / Q000096:biosynthesis* / Q000187:drug effects; D004279:DNA, Viral / Q000032:analysis / Q000737:chemistry / Q000187:drug effects*; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000032:analysis; D012965:Sodium Chloride / Q000494:pharmacology*
D004279:DNA, Viral; D009841:Oligonucleotides; D001761:Bleomycin; D012965:Sodium Chloride; D004247:DNA