title: A mixture of paraphenylenediamine and imidazole: its effect on the extraction of lipid droplets during electron microscopy staining.
abstract: To prevent extraction of lipids during a double staining procedure for electron microscopy, the tissue slices, double fixed with glutaraldehyde and osmium tetroxide to preserve microvesicular lipid droplets in the cytoplasm, were immersed for 2 hr in veronal buffer (pH 9.0) containing 0.5% p-phenylenediamine and 0.5% imidazole immediately after postfixation. The stained sections of the immersed tissue slice showed blackened, well circumscribed lipid droplets similar to those in corresponding unstained sections. Moreover, highly contrasting features of the cellular architecture could be visualized with the double stained, as well as routinely prepared sections.
authors.affiliation: Department of Pathology, Kansai Medical University, Osaka, Japan.
authors.forename: I
authors.initials: I
authors.lastname: Nakao
authors.forename: H
authors.initials: H
authors.lastname: Okada
authors.forename: H
authors.initials: H
authors.lastname: Senzaki
authors.forename: R
authors.initials: R
authors.lastname: Nishimura
authors.forename: N
authors.initials: N
authors.lastname: Fujita
authors.forename: N
authors.initials: N
authors.lastname: Shikata
authors.forename: S
authors.initials: S
authors.lastname: Morii
mesh_terms: D000302:Adrenal Cortex / Q000166:cytology; D000818:Animals; D005260:Female; D007093:Imidazoles; D008055:Lipids / Q000032:analysis*; D008297:Male; D008854:Microscopy, Electron / Q000379:methods; D008867:Microtomy; D010655:Phenylenediamines; D051381:Rats; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D007093:Imidazoles; D008055:Lipids; D010655:Phenylenediamines; C029899:imidazole; C029728:4-phenylenediamine