Combined action of c-kit and erythropoietin on erythroid progenitor cells.
Mutations at the murine dominant-white spotting locus (W) (c-kit) affect various aspects of hematopoiesis. We have made antibodies against c-Kit with the synthetic peptides deduced from the murine c-kit gene and examined the role of c-Kit in erythropoiesis. The antibody inhibited the stromal cell-dependent large colony formation of the erythroid progenitors. In the culture of erythropoietin-responsive erythroid progenitors of the anemia-inducing Friend virus-infected mouse spleen, the antibody inhibited only proliferation, but not differentiation of the progenitor cells. The inhibition was effective only at the early phase (within 6 hours after erythropoietin addition) before the cells start to proliferate induced by erythropoietin. During the early phase, erythropoietin down-regulated c-kit gene expression. These results suggest a mechanism of combined action of c-Kit with erythropoietin on the lineage-restricted erythroid progenitor cells.
Ohneda
O
O
Department of Cell Biology, Tohoku University, Sendai, Japan.
Yanai
N
N
Obinata
M
M
D000818:Animals; D000911:Antibodies, Monoclonal; D002454:Cell Differentiation / Q000502:physiology; D002455:Cell Division / Q000502:physiology; D015672:Erythroid Precursor Cells / Q000502:physiology* / Q000648:ultrastructure; D004920:Erythropoiesis / Q000502:physiology*; D004921:Erythropoietin / Q000502:physiology*; D005786:Gene Expression Regulation / Q000502:physiology; D005838:Genotype; D051379:Mice; D009154:Mutation / Q000235:genetics; D011518:Proto-Oncogene Proteins / Q000276:immunology / Q000502:physiology*; D019009:Proto-Oncogene Proteins c-kit; D013997:Time Factors
D000911:Antibodies, Monoclonal; D011518:Proto-Oncogene Proteins; D004921:Erythropoietin; D019009:Proto-Oncogene Proteins c-kit