Isolation and characterization of bovine gingival proteoglycans versican and decorin.
1. We have isolated, chemically and immunologically characterized versican and decorin from bovine gingiva. 2. Versican was of large molecular weight and the molecular size of the core protein was estimated to be greater than 200 kDa. 3. The glycosaminoglycan chains were susceptible to chondroitinase ABC and N-linked oligosaccharides were present on the protein core of the molecule. 4. Immunological studies provided evidence that a hyaluronic acid binding region was present in the core protein of versican. 5. The overall structure was similar to that of versican isolated from bovine sclera. 6. Decorin had a molecular weight of 102 kDa and its glycosaminoglycan chain was completely digested by specific glycosidases. 7. The partially deglycosylated core protein had a molecular weight of 55 kDa and N-linked oligosaccharides were present on the molecule.
Bratt
P
P
Department of Community and Public Health Dentistry, University of Alabama School of Dentistry, University Station, Birmingham 35294.
Anderson
M M
MM
Månsson-Rahemtulla
B
B
Stevens
J W
JW
Zhou
C
C
Rahemtulla
F
F
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002417:Cattle; D011508:Chondroitin Sulfate Proteoglycans / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D058575:Decorin; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D016326:Extracellular Matrix Proteins; D005881:Gingiva / Q000737:chemistry*; D037181:Lectins, C-Type; D011509:Proteoglycans / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*; D053675:Versicans
D000596:Amino Acids; D011508:Chondroitin Sulfate Proteoglycans; D058575:Decorin; D016326:Extracellular Matrix Proteins; D037181:Lectins, C-Type; D011509:Proteoglycans; D053675:Versicans