Molecular analysis of virus-producing and non-producing clones derived from a defective SSPE virus Yamagata-1 strain.
Two virus clones were isolated from a defective SSPE virus, the Yamagata-1 strain, and designated as the YA and YF clones. The YA clone-infected cells produced neither cell-free virus nor cell-associated virus, whereas the YF clone-infected cells produced both cell-associated and cell-free virus. No difference of epitopes on structural proteins was observed between these two clones. Both clones had hemadsorption activity. Quantitation of structural protein by Western dot blots showed relatively a lower amount of M protein in the YA-infected cells than that in the YF-infected cells. The ratio, P plus M dicistronic/M monocistronic mRNA, in the YA-infected cells was about twice that in the YF-infected cells. Sequence analysis of cDNA corresponding to P plus M dicistronic mRNA revealed that the deduced M protein of the YF virus was smaller than that of the YA virus by five amino acids from the carboxy terminal. These results suggest that abundant production of P plus M dicistronic mRNA is responsible for the reduced amount of M protein in the non-productive YA clone.
Haga
T
T
Laboratory Animal Research Center, University of Tokyo, Japan.
Komase
K
K
Yoshikawa
Y
Y
Yamanouchi
K
K
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D001483:Base Sequence; D015153:Blotting, Western; D002999:Clone Cells; D004279:DNA, Viral / Q000235:genetics; D000939:Epitopes; D006383:Hemadsorption Inhibition Tests; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics; D013190:SSPE Virus / Q000235:genetics*; D014709:Vero Cells; D015678:Viral Structural Proteins / Q000032:analysis
D000911:Antibodies, Monoclonal; D004279:DNA, Viral; D000939:Epitopes; D012333:RNA, Messenger; D015678:Viral Structural Proteins