Quantitation of phenacyl esters of retinal fatty acids by high-performance liquid chromatography.
A high-performance liquid chromatographic (HPLC) method for the separation and quantitation of retinal fatty acids containing long-chain polyunsaturated fatty acids is described. Fatty acids from frog retinal lipids were converted to the corresponding phenacyl derivatives which were separated on a C18 reversed-phase column and detected at 242 nm. Molar absorptivities (peak area units/nmol) of up to seventeen fatty acid phenacyl derivatives were determined and used for quantitation of fatty acids separated by HPLC. Compared with gas chromatography, the HPLC method gave a similar molar percent distribution of the fatty acids and was twenty to fifty times more sensitive. This HPLC method provides a useful means for the study of chemistry and metabolism of long-chain polyunsaturated fatty acids in retina and other tissues where amounts of material may be limited or recovery of individual components desirable.
Chen
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Department of Biochemistry, Baylor College of Medicine, Houston, TX 77030.
Anderson
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D000098:Acetophenones / Q000032:analysis*; D000818:Animals; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D005227:Fatty Acids / Q000032:analysis; D005231:Fatty Acids, Unsaturated / Q000032:analysis*; D011894:Rana pipiens; D012160:Retina / Q000737:chemistry*
D000098:Acetophenones; D005227:Fatty Acids; D005231:Fatty Acids, Unsaturated