[Effects of exogenous poly(A)+ mRNA on antigenic properties and growth of Krebs-2 tumor cells].
The treatment of Krebs-2 ascitic tumor cells (TC) with total RNA from the liver of Wistar rats (2 mg/ml) altered their antigenicity. As a result, the growth of treated TC in contrast with untreated TC was inhibited when transplanted i. m. to mice preimmunized with rat liver homogenate. Investigations of poly(A+)mRNA, rRNA and tRNA isolated from the same tissue established that the alteration of antigenicity is due to mRNA (8-24 micrograms/ml). In the cytotoxicity assay with antisera against rat Wistar antigens, the expression of rat antigens in TC treated mRNA was observed in the next cell generations.
Nikolin
V P
VP
Il'nitskaia
S I
SI
Vishnivetskiĭ
S N
SN
Mertvetsov
N P
NP
D000818:Animals; D002287:Carcinoma, Krebs 2 / Q000276:immunology* / Q000473:pathology; D003601:Cytotoxicity Tests, Immunologic; D000939:Epitopes; D007114:Immunization; D007165:Immunosuppression Therapy; D008099:Liver / Q000737:chemistry; D016031:Liver Transplantation; D051379:Mice; D008808:Mice, Inbred CBA; D008815:Mice, Inbred Strains; D011061:Poly A / Q000235:genetics / Q000276:immunology; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics / Q000276:immunology; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D014407:Tumor Cells, Cultured
D000939:Epitopes; D012333:RNA, Messenger; D011061:Poly A