Characterization of the submicroscopic deletion in the small-cell lung carcinoma (SCLC) cell line U2020.
The small-cell lung carcinoma cell line U2020 contains a submicroscopic, homozygous deletion that removes a chromosomal segment within 3p13-p14, including the locus D3S3. We have sublocalized 49 additional probes to the 3p13-p14.2 region and have identified 7 new DNA markers that arise from within the U2020 deletion. The estimated size of the deletion, based on marker density, is approximately 4-5 megabases (Mb). Including D3S3, 7 of the 8 markers have been linked by pulsed-field gel (PFG) electrophoresis over an area of approximately 2 Mb. Including the one unlinked marker, PFG analysis accounts for about 3 Mb of the region. The U2020 deletion appears confined to the 3p13-p14.2 region and does not include the candidate tumor suppressor gene, protein-tyrosine phosphatase gamma (PTPG).
Drabkin
H A
HA
Division of Medical Oncology, University of Colorado Cancer Center, Denver 80262.
Mendez
M J
MJ
Rabbitts
P H
PH
Varkony
T
T
Bergh
J
J
Schlessinger
J
J
Erickson
P
P
Gemmill
R M
RM
D018288:Carcinoma, Small Cell / Q000235:genetics* / Q000473:pathology; D002872:Chromosome Deletion; D002893:Chromosomes, Human, Pair 3 / Q000648:ultrastructure*; D015342:DNA Probes; D016521:Electrophoresis, Gel, Pulsed-Field; D008040:Genetic Linkage; D005819:Genetic Markers; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology; D017027:Protein Tyrosine Phosphatases / Q000235:genetics*; D014407:Tumor Cells, Cultured
D015342:DNA Probes; D005819:Genetic Markers; D017027:Protein Tyrosine Phosphatases