title: Rapid effects of insulin on cyclic GMP location in an intact protozoan.
abstract: We studied rapid changes in location of cyclic GMP in Tetrahymena pyriformis. Insulin caused cGMP localization in cilia and near the plasma membrane (0.5-1 min). Later (1-5 min) cGMP localization was diffuse in cytoplasm with perinuclear accentuation. Inactive insulin analogs did not elicit these changes.
authors.affiliation: Metabolic Diseases Branch, NIDDK, Bethesda, Maryland 20892.
authors.forename: L
authors.initials: L
authors.lastname: Kohidai
authors.forename: J
authors.initials: J
authors.lastname: Barsony
authors.forename: J
authors.initials: J
authors.lastname: Roth
authors.forename: S J
authors.initials: SJ
authors.lastname: Marx
mesh_terms: D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D002923:Cilia / Q000187:drug effects / Q000378:metabolism; D006152:Cyclic GMP / Q000378:metabolism*; D003593:Cytoplasm / Q000187:drug effects / Q000378:metabolism; D007328:Insulin / Q000031:analogs & derivatives / Q000494:pharmacology*; D009599:Nitroprusside / Q000494:pharmacology; D013769:Tetrahymena pyriformis / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D014018:Tissue Distribution
chemical_list: D007328:Insulin; D009599:Nitroprusside; D006152:Cyclic GMP; D015056:1-Methyl-3-isobutylxanthine