title: Variability of crossreactivity of IgE antibodies to group I and V allergens in eight grass pollen species.
abstract: Crossreactivity to Dactylis glomerata, Festuca rubra, Phleum pratense, Anthoxanthum odoratum, Secale cereale, Zea mays, and Phragmites communis of IgE antibodies against Lol p I or Lol p V was investigated by means of RAST-inhibition. Within a group of sera the degree of crossreactivity was demonstrated to be highly variable. Individual sera were not always equally crossreactive to all pollen species. A high degree of crossreactivity for Group I allergens did not necessarily implicate the same for Group V. Group I and Group V representatives were found to be present in all eight species. It was demonstrated that within this group of grass species significant quantitative and qualitative differences exist, with respect to Group I and Group V allergens. Species with a low phylogenetic affinity to Lolium perenne, like Zea mays and Phragmites communis showed a very low degree of reactivity, even when measured with the most crossreactive sera. A higher taxonomic relationship however, did not always implicate a closer antigenic resemblance. Antigenically both allergens from Zea mays are more similar to Lol p I and Lol p V, than the analogues in Secale cereale.
authors.affiliation: Central Laboratory of The Netherlands Red Cross Blood Transfusion Service, Amsterdam.
authors.forename: R
authors.initials: R
authors.lastname: Van Ree
authors.forename: M N
authors.initials: MN
authors.lastname: Driessen
authors.forename: W A
authors.initials: WA
authors.lastname: Van Leeuwen
authors.forename: S O
authors.initials: SO
authors.lastname: Stapel
authors.forename: R C
authors.initials: RC
authors.lastname: Aalberse
mesh_terms: D000485:Allergens / Q000276:immunology*; D000940:Antigenic Variation / Q000276:immunology; D003429:Cross Reactions / Q000276:immunology*; D000939:Epitopes / Q000276:immunology; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology*; D006109:Poaceae; D011058:Pollen / Q000276:immunology*; D011852:Radioallergosorbent Test
chemical_list: D000485:Allergens; D000939:Epitopes; D007073:Immunoglobulin E