Single cell observation of ligand-induced desensitization of B-cell membrane immunoglobulin-mediated calcium signals.
Using a digital imaging fluorescence microscope we have observed the membrane immunoglobulin (mIg)-induced desensitization of calcium signals in individual BAL17 B lymphoma cells which express two kinds of antigen receptors, mIgM and mIgD. The mIgD-mediated desensitization was partly abrogated by pretreating the cells with phorbol 12-myristate 13-acetate (PMA) for 24 h, however, the mIgM-mediated one was not affected by the pretreatment. This supports the idea that at least two mechanisms are operative for mIg-induced desensitization in B cells.
Yamada
H
H
Faculty of Pharmaceutical Sciences, Nagoya City University, Japan.
Mizuguchi
J
J
Noji
M
M
Nakanishi
M
M
D001402:B-Lymphocytes / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism*; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D007136:Immunoglobulins / Q000378:metabolism*; D008024:Ligands; D008856:Microscopy, Fluorescence; D011493:Protein Kinase C / Q000378:metabolism; D015398:Signal Transduction
D007136:Immunoglobulins; D008024:Ligands; D011493:Protein Kinase C; D002118:Calcium