A new restriction fragment length polymorphism of the human alpha-fetoprotein gene in Japanese individuals: detection of loss of heterozygosity in human hepatocellular carcinoma.
By using the cDNA probe for the human alpha-fetoprotein (AFP) gene, one restriction fragment length polymorphism (RFLP) was discovered in Japanese patients. A variety of combinations of restriction enzymes and probes were evaluated in DNA samples from 25 Japanese individuals who were genetically unrelated. As a result, AvaII identified one invariant band of 8 kb and polymorphic bands at either 2.8 or 2.0 kb with the 5'-region probe from a cDNA clone, pHAF7. With the AvaII/pHAF7 combination, we examined the loss of heterozygosity in 14 patients with hepatocellular carcinoma (HCC). Of 6 informative specimens, 2 showed loss of heterozygosity. These data suggest that the AFP cDNA probe could be useful for analyzing loss of heterozygosity on chromosome 4q in human HCC.
Toyota
M
M
Department of Internal Medicine, Sapporo Medical College, Japan.
Hinoda
Y
Y
Nakano
T
T
Imai
K
K
Yachi
A
A
D000368:Aged; D006528:Carcinoma, Hepatocellular / Q000235:genetics*; D002872:Chromosome Deletion; D006580:Genetic Carrier Screening; D006579:Heterozygote; D006801:Humans; D007564:Japan / Q000208:ethnology; D008113:Liver Neoplasms / Q000235:genetics*; D008875:Middle Aged; D012150:Polymorphism, Restriction Fragment Length; D000509:alpha-Fetoproteins / Q000235:genetics*
D000509:alpha-Fetoproteins