title: Spectrum of sialylated and nonsialylated fuco-oligosaccharides bound by the endothelial-leukocyte adhesion molecule E-selectin. Dependence of the carbohydrate binding activity on E-selectin density.
abstract: Carbohydrate recognition by the human endothelial-leukocyte adhesion molecule, E-selectin, has been investigated by binding studies using 3H-labeled Chinese hamster ovary cells expressing different levels of the transfected full-length adhesion molecule and a series of structurally defined oligosaccharides linked to the lipid phosphatidylethanolamine dipalmitoate (neoglycolipids) and synthetic glycolipids chromatographed on silica gel plates or immobilized on plastic wells. Evidence is presented for density-dependent binding of the membrane-associated E-selectin not only to 3'-sialyl-lacto-N-fucopentaose II (3'-S-LNFP-II) and 3'-sialyl-lacto-N-fucopentaose III (3'-S-LNFP-III) which express the sialyl Le(a) and sialyl Le(x) antigens, respectively, but also to the nonsialylated analogue LNFP-II; there is a threshold density of E-selectin required for binding to these sialylated sequences, and binding to the nonsialylated analogue is a property only of cells with the highest density of E-selectin expression. The presence of fucose linked to subterminal rather than to an internal N-acetylglucosamine is shown to be a requirement for E-selectin binding, and although the presence of sialic acid 3-linked to the terminal galactose of the LNFP-II or LNFP-III sequences substantially enhances E-selectin binding, the presence of 6-linked sialic acid abolishes binding. E-selectin binding is unaffected in the presence of the blood group H fucose (alpha 1-2 linked to galactose to form the Le(b) antigen). However, the binding is abolished when in addition alpha 1-3-linked N-acetylgalactosamine to the galactose (blood group A antigen) is present. These results indicate that some E-selectin-mediated adhesive events may be influenced by blood group status.
authors.affiliation: Glycoconjugates Section, Medical Research Council Clinical Research Centre, Harrow, Middlesex, United Kingdom.
authors.forename: M
authors.initials: M
authors.lastname: Larkin
authors.forename: T J
authors.initials: TJ
authors.lastname: Ahern
authors.forename: M S
authors.initials: MS
authors.lastname: Stoll
authors.forename: M
authors.initials: M
authors.lastname: Shaffer
authors.forename: D
authors.initials: D
authors.lastname: Sako
authors.forename: J
authors.initials: J
authors.lastname: O'Brien
authors.forename: C T
authors.initials: CT
authors.lastname: Yuen
authors.forename: A M
authors.initials: AM
authors.lastname: Lawson
authors.forename: R A
authors.initials: RA
authors.lastname: Childs
authors.forename: K M
authors.initials: KM
authors.lastname: Barone
mesh_terms: D000818:Animals; D016466:CHO Cells; D050260:Carbohydrate Metabolism; D002240:Carbohydrate Sequence; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D002853:Chromatography, Liquid; D002855:Chromatography, Thin Layer; D006224:Cricetinae; D004247:DNA; D019040:E-Selectin; D016256:Lewis X Antigen / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: D015815:Cell Adhesion Molecules; D019040:E-Selectin; D016256:Lewis X Antigen; D009844:Oligosaccharides; C058981:sialyl-Le(a) oligosaccharide; C041762:lacto-N-fucopentaose II; D004247:DNA