Production and characterization of a monoclonal antibody against the ribosome inactivating protein alpha sarcin.
In order to improve the purification of immunoconjugates containing alpha sarcin, a ribosome-inactivating protein, and in the attempt to define the enzymic region of the toxin, MAbs against alpha sarcin were produced. From 5 fusions, by adopting a short period of immunization and very low doses of the immunogen, 10 anti-toxin-producing clones were obtained. One of them, named MAsg2 (IgG2b), due to its specific reactivity and secreting properties, was selected for further characterization. MAsg2 was found to recognize an epitope which is common to two, i.e. alpha sarcin and clavatin, of the three different aspergillins tested, but is not involved in the active site of the toxins.
Ferreri
A J
AJ
Division of Experimental Oncology E, Istituto Nazionale per lo Studio e la Cura dei Tumori, Milan, Italy.
Ferraris di Celle
E
E
Caldera
M
M
Muñoz
S
S
Salvarelli
S
S
Conde
F
F
Canevari
S
S
Tosi
E
E
Colnaghi
M I
MI
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000302:isolation & purification; D000946:Antigens, Fungal; D001230:Aspergillus; D004722:Endoribonucleases; D000939:Epitopes; D005656:Fungal Proteins / Q000276:immunology* / Q000633:toxicity; D006825:Hybridomas / Q000276:immunology; D007114:Immunization; D000922:Immunotoxins / Q000302:isolation & purification; D051379:Mice; D011500:Protein Synthesis Inhibitors / Q000276:immunology / Q000633:toxicity; D012270:Ribosomes / Q000187:drug effects
D000911:Antibodies, Monoclonal; D000946:Antigens, Fungal; D000939:Epitopes; D005656:Fungal Proteins; D000922:Immunotoxins; D011500:Protein Synthesis Inhibitors; C014801:alpha-sarcin; D004722:Endoribonucleases