title: The distribution of UV damage in the lacI gene of Escherichia coli: correlation with mutation spectrum.
abstract: We have determined the UV (254 nm) damage distribution in the transcribed and non-transcribed strands of the i-d region of the Escherichia coli lacI gene. The locations of replication blocking lesions were revealed as termination sites of T7 DNA polymerase and/or T4 DNA polymerase 3'-5' exonuclease. Termination products, i.e. both cyclobutane pyrimidine dimers and 6-4 photoproducts, were resolved and analysed on an automated DNA sequencer. These two major photoproducts are not randomly distributed along the gene, but occur in clusters, in longer runs of pyrimidines. We also have compared the UV damage distribution with the previously reported UV-induced base substitutions in the same region. Mutational hotspots, in both repair-deficient and repair-proficient strains of E. coli, are all located in stretches of pyrimidines, and thus correlate well with the distribution of photolesions. One mutational hotspot in the wild-type strain may reflect the high frequency of closely opposed lesions. To explain the other mutational hotspots, we propose that the repair of UV lesions is impaired due to the local conformation of the DNA, which might deviate from the B-form. This hypothesis is supported by the excess of mutational hotspots in pyrimidine runs in the Uvr+ strain compared to Uvr-. Runs of pyrimidines thus represent both damage- and mutation-prone sequences following UV treatment.
authors.affiliation: York University, Department of Biology, Ont., Canada.
authors.forename: E
authors.initials: E
authors.lastname: Sage
authors.forename: E
authors.initials: E
authors.lastname: Cramb
authors.forename: B W
authors.initials: BW
authors.lastname: Glickman
mesh_terms: D001483:Base Sequence; D004249:DNA Damage; D004260:DNA Repair; D004269:DNA, Bacterial / Q000235:genetics / Q000528:radiation effects; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics* / Q000528:radiation effects*; D005798:Genes, Bacterial / Q000528:radiation effects*; D008969:Molecular Sequence Data; D016296:Mutagenesis; D011740:Pyrimidine Dimers / Q000032:analysis; D014466:Ultraviolet Rays
chemical_list: D004269:DNA, Bacterial; D011740:Pyrimidine Dimers; D004259:DNA-Directed DNA Polymerase