Extraction and characterization of lipopolysaccharide from Pseudomonas pseudomallei.
The best yield of lipopolysaccharide (LPS) of Pseudomonas pseudomallei GIFU 12046 was obtained by extraction of defatted cells by phenol/chloroform/petroleum ether. The LPS showed a smooth character on SDS-polyacrylamide gel electrophoresis and contained D-glucose, L-glycero-D-manno-heptose, and D-glucosamine as the main sugar components, and 3-hydroxypalmitic acid as an amide-linked fatty acid. The growth conditions did not affect the electrophoresis profile and chemical composition of LPS. 2-Keto-3-deoxyoctonic acid was not detectable, and mild acid hydrolysis could not liberate free lipid A, suggesting that the linkage between inner core and lipid A was stable against acid hydrolysis, and the structure of this region is similar to that of P. cepacia, which has close taxonomic relationship with P. pseudomallei.
Kawahara
K
K
Department of Bacteriology, Kitasato Institute, Tokyo, Japan.
Dejsirilert
S
S
Danbara
H
H
Ezaki
T
T
D016957:Burkholderia pseudomallei / Q000737:chemistry* / Q000145:classification / Q000472:pathogenicity; D002240:Carbohydrate Sequence; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D006868:Hydrolysis; D008070:Lipopolysaccharides / Q000737:chemistry / Q000302:isolation & purification*; D008554:Melioidosis / Q000209:etiology; D008969:Molecular Sequence Data; D014774:Virulence
D008070:Lipopolysaccharides