The 26 S proteasome is activated at two points in the ascidian cell cycle.
The proteasome undergoes cell cycle-dependent changes in its subcellular distribution during ascidian embryonic development [(1992) Dev. Biol. 151, 27-33]. In this study, we demonstrate that the 26 S proteasome is markedly activated in both prophase and metaphase of the mitotic cell cycle in the ascidian embryos in comparison with the case of the 20 S proteasome. These results suggest that the 26 S proteasome is activated and participates in proteolysis at the restricted stages of the cell cycle.
Kawahara
H
H
Department of Biochemistry, Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo Japan.
Sawada
H
H
Yokosawa
H
H
D000818:Animals; D015153:Blotting, Western; D002453:Cell Cycle; D002850:Chromatography, Gel; D002918:Chymotrypsin / Q000378:metabolism; D003546:Cysteine Endopeptidases / Q000378:metabolism*; D004789:Enzyme Activation; D009097:Multienzyme Complexes / Q000378:metabolism*; D046988:Proteasome Endopeptidase Complex; D014561:Urochordata / Q000166:cytology / Q000201:enzymology*
D009097:Multienzyme Complexes; D002918:Chymotrypsin; D003546:Cysteine Endopeptidases; D046988:Proteasome Endopeptidase Complex