title: A cytotoxic monoclonal human hybridoma antibody (TrJ3) against HLA-B44(12) and -B45(12).
abstract: We have generated a human monoclonal cytotoxic IgM lambda antibody (TrJ3) that reacted specifically with all lymphoblastoid B-cell lines expressing HLA-B44(12) and B45(12). TrJ3 hybridoma supernatant was suitable for HLA-B12 typing of freshly isolated blood mononuclear cells. Analysis of available amino acid sequences of HLA-B molecules indicated that the alpha 1 domain does not contain the TrJ3 serological epitope. Since HLA-B44 is associated with a unique serine residue at position 167 that points towards the peptide binding groove, we propose that S167 of the alpha 2 domain helix is a critical part of the TrJ3 epitope.
authors.affiliation: Department of Immunology, University of Tromsø, School of Medicine, Norway.
authors.forename: J
authors.initials: J
authors.lastname: Ge
authors.forename: A
authors.initials: A
authors.lastname: Bratlie
authors.forename: K
authors.initials: K
authors.lastname: Hannestad
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D001666:Binding Sites, Antibody; D003602:Cytotoxicity, Immunologic; D000939:Epitopes / Q000235:genetics / Q000276:immunology; D005787:Gene Frequency; D005802:Genes, MHC Class II; D015235:HLA-B Antigens / Q000235:genetics / Q000276:immunology*; D059908:HLA-B13 Antigen; D059948:HLA-B44 Antigen; D006650:Histocompatibility Testing; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007075:Immunoglobulin M / Q000276:immunology*; D007146:Immunoglobulin lambda-Chains / Q000276:immunology; D007963:Leukocytes, Mononuclear / Q000276:immunology; D051379:Mice; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D017385:Sequence Homology
chemical_list: D000911:Antibodies, Monoclonal; D000939:Epitopes; D015235:HLA-B Antigens; C035015:HLA-B12 antigen; D059908:HLA-B13 Antigen; D059948:HLA-B44 Antigen; C077377:HLA-B45 antigen; C103707:HLA-B57 antigen; D007075:Immunoglobulin M; D007146:Immunoglobulin lambda-Chains