title: The presence of scatter factor in patients with metastatic spread to the pleura.
abstract: Pleural effusion fluid obtained from eleven patients with metastatic spread to the pleura was screened for the ability to cause the dispersal--'scattering'--of MDCK colonies in vitro. Four of these samples proved to be positive using this assay. Of these two had titres high enough to warrant further purification on a cation exchange Mono S column. Active material from both lung samples, eluted at the same positions as factor from cultured human lung fibroblasts (MRC-5) and human placenta but in a slightly different position to murine scatter factor. In both cases the semi-purified active agent was identified as hepatocyte growth factor/scatter factor (HGF/SF) using an ELISA detection system specific for human HGF/SF. This is the first report identifying the presence of significant amounts of HGF/SF in the pleura of patients where malignant spread has occurred.
authors.affiliation: School of Biology, University of East Anglia, Norwich, UK.
authors.forename: P
authors.initials: P
authors.lastname: Kenworthy
authors.forename: P
authors.initials: P
authors.lastname: Dowrick
authors.forename: H
authors.initials: H
authors.lastname: Baillie-Johnson
authors.forename: B
authors.initials: B
authors.lastname: McCann
authors.forename: H
authors.initials: H
authors.lastname: Tsubouchi
authors.forename: N
authors.initials: N
authors.lastname: Arakaki
authors.forename: Y
authors.initials: Y
authors.lastname: Daikuhara
authors.forename: R M
authors.initials: RM
authors.lastname: Warn
mesh_terms: D000818:Animals; D001943:Breast Neoplasms / Q000473:pathology / Q000503:physiopathology; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects; D016207:Cytokines / Q000302:isolation & purification* / Q000494:pharmacology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D017228:Hepatocyte Growth Factor; D006801:Humans; D008175:Lung Neoplasms / Q000473:pathology / Q000503:physiopathology; D009362:Neoplasm Metastasis / Q000503:physiopathology*; D016066:Pleural Effusion, Malignant / Q000503:physiopathology*; D002583:Uterine Cervical Neoplasms / Q000473:pathology / Q000503:physiopathology
chemical_list: D016207:Cytokines; D017228:Hepatocyte Growth Factor