Purification and characterization of fibroblast growth factors from the opossum, Monodelphis domestica, brain.
An extract from the brain of the opossum Monodelphis domestica was fractionated by heparin affinity chromatography. A major peak of mitogenic activity (heparin binding growth factor 2, HBGF-2) eluted from heparin-Sepharose between 1.7 and 2.0 M NaCl. Antisera specific for bovine bFGF detected four polypeptides of 17.5-23 kDa in opossum brain HBGF-2 preparations. Opossum brain heparin binding growth factor 1 (HBGF-1), a minor peak of activity, eluted from heparin-Sepharose at 1.1 NaCl and contained a 16.2 kDa protein that cross-reacted with antiserum against bovine aFGF.
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Center for Basic and Applied Medical Research, Lovelace Medical Foundation, Albuquerque, NM 87108.
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D016475:3T3 Cells; D000818:Animals; D001681:Biological Assay; D015152:Blotting, Northern; D001923:Brain Chemistry; D002846:Chromatography, Affinity; D016220:Fibroblast Growth Factor 1 / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*; D016222:Fibroblast Growth Factor 2 / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*; D015151:Immunoblotting; D051379:Mice; D008970:Molecular Weight; D009893:Opossums
D016222:Fibroblast Growth Factor 2; D016220:Fibroblast Growth Factor 1