title: Functional properties of human hemoglobins synthesized from recombinant mutant beta-globins.
abstract: The previous and following articles in this issue describe the recombinant synthesis of three mutant beta-globins (beta 1 Val----Ala, beta 1 Val----Met, and the addition mutation beta 1 + Met), their assembly with heme and natural alpha chains into alpha 2 beta 2 tetramers, and their X-ray crystallographic structures. Here we have measured the equilibrium and kinetic allosteric properties of these hemoglobins. Our objective has been to evaluate their utility as surrogates of normal hemoglobin from which further mutants can be made for structure-function studies. The thermodynamic linkages between cooperative oxygenation and dimer-tetramer assembly were determined from global regression analysis of multiple oxygenation isotherms measured over a range of hemoglobin concentration. Oxygen binding to the tetramers was found to be highly cooperative (maximum Hill slopes from 3.1 to 3.2), and similar patterns of O2-linked subunit assembly free energies indicated a common mode of cooperative switching at the alpha 1 beta 2 interface. The dimers were found to exhibit the same noncooperative O2 equilibrium binding properties as normal hemoglobin. The most obvious difference in oxygen equilibria between the mutant recombinant and normal hemoglobins was a slightly lowered O2 affinity. The kinetics of CO binding and O2 dissociation were measured by stopped-flow and flash photolysis techniques. Parallel studies were carried out with the mutant and normal hemoglobins in the presence and absence of organic phosphates to assess their allosteric response to phosphates. In the absence of organic phosphates, the CO-binding and O2 dissociation kinetic properties of the mutant dimers and tetramers were found to be nearly identical to those of normal hemoglobin. However, the effects of organic phosphates on CO-binding kinetic properties of the mutants were not uniform: the beta 1 + Met mutant was found to deviate somewhat from normalcy, while the beta 1 Val----Met mutant reproduced the native allosteric response. Further characterization of the allosteric properties of the beta 1 Val----Met mutant was made by measuring the pH dependence of its overall oxygen affinity by tonometry. Regulation of oxygen affinity by protons was found to be nearly identical to normal hemoglobin from pH 5.8 to 9.3 (0.52 +/- 0.07 protons released per oxygen bound at pH 7.4). The present study demonstrates that the equilibrium and kinetic functional properties of the recombinant beta 1 Val----Met mutant mimic reasonably well those of normal hemoglobin. We conclude that this mutant is well-suited to serve as a surrogate system of normal hemoglobin in the production of mutants for structure-function studies.
authors.affiliation: Department of Biochemistry and Molecular Biophysics, School of Medicine, Washington University, St. Louis, Missouri 63110.
authors.forename: M L
authors.initials: ML
authors.lastname: Doyle
authors.forename: G
authors.initials: G
authors.lastname: Lew
authors.forename: A
authors.initials: A
authors.lastname: De Young
authors.forename: L
authors.initials: L
authors.lastname: Kwiatkowski
authors.forename: A
authors.initials: A
authors.lastname: Wierzba
authors.forename: R W
authors.initials: RW
authors.lastname: Noble
authors.forename: G K
authors.initials: GK
authors.lastname: Ackers
mesh_terms: D000494:Allosteric Regulation; D002248:Carbon Monoxide / Q000378:metabolism; D004734:Energy Metabolism; D005914:Globins / Q000096:biosynthesis / Q000378:metabolism; D006454:Hemoglobins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D046911:Macromolecular Substances; D008956:Models, Chemical; D009154:Mutation; D010100:Oxygen / Q000378:metabolism; D010710:Phosphates / Q000378:metabolism; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000378:metabolism
chemical_list: D006454:Hemoglobins; D046911:Macromolecular Substances; D010710:Phosphates; D011994:Recombinant Proteins; D002248:Carbon Monoxide; D005914:Globins; D010100:Oxygen