Specific increase of a mitochondrial RNA transcript in chronic ethanol-fed rats.
An in vitro transcription system utilizing isolated mitochondria has been used to study the effect of chronic ethanol consumption on liver mitochondrial DNA transcription. The results obtained showed an overall increase of RNA synthesis and a dramatic accumulation of a discrete polyadenylated RNA species. This effect is a consequence of the chronic ethanol consumption since these changes do not occur when isolated control mitochondria are incubated in the presence of ethanol.
Enríquez
J A
JA
Departamento de Bioquímica y Biología Molecular y Celular, Facultad de Veterinaria, Universidad de Zaragoza, Spain.
Pérez-Martos
A
A
Fernández-Silva
P
P
López-Pérez
M J
MJ
Montoya
J
J
D000437:Alcoholism / Q000503:physiopathology*; D000818:Animals; D004272:DNA, Mitochondrial / Q000378:metabolism; D008297:Male; D008930:Mitochondria, Liver / Q000187:drug effects / Q000378:metabolism*; D011061:Poly A / Q000378:metabolism; D012313:RNA / Q000096:biosynthesis*; D012323:RNA Processing, Post-Transcriptional; D051381:Rats
D004272:DNA, Mitochondrial; D011061:Poly A; D012313:RNA