Rapid analysis of human serum albumin by high-performance liquid chromatography.
High-performance liquid chromatographic analysis of human serum albumin, using a column containing quaternized dimethyl-aminomethylstyrene-ethylene glycol dimethacrylate, was performed by isocratic elution. This column afforded resolution of albumin components, such as human mercaptalbumin and human nonmercaptalbumin. The method is an alternative to gradient chromatography, and allows rapid determination of the albumin components.
Etoh
T
T
Faculty of Pharmaceutical Sciences, Kumamoto University, Japan.
Miyazaki
M
M
Harada
K
K
Nakayama
M
M
Sugii
A
A
D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006435:Renal Dialysis; D012709:Serum Albumin / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet
D012709:Serum Albumin