Isolation of human retinal genes: recoverin cDNA and gene.
A human retina cDNA library enriched for retina-specific clones was prepared by subtraction with a non-retina population of cDNA in combination with polymerase chain reaction (PCR) amplifications. A highly retina-specific cDNA clone (1190 bp) was obtained through this library encoding a 200 amino acid protein with three calcium binding sites and 87% homology to the bovine photoreceptor protein, recoverin, which has been shown to mediate the recovery of the dark current after photoactivation, and 58% homology to the calcium-binding chick cone protein, visinin. Analysis of the gene indicated a 9-10 kb single-copy gene with at least three exons and two introns. The three exons contained the entire coding sequence, and all of the calcium-binding EF-hand regions were in putative exon 1. The recoverin gene was mapped to human chromosome 17 by hybridization to a panel of human-rodent hybrid DNAs.
Murakami
A
A
Bascom Palmer Eye Institute, University of Miami School of Medicine, FL 33136.
Yajima
T
T
Inana
G
G
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D015139:Blotting, Southern; D002118:Calcium / Q000378:metabolism; D002135:Calcium-Binding Proteins / Q000235:genetics*; D002417:Cattle; D002645:Chickens; D002886:Chromosomes, Human, Pair 17; D004247:DNA / Q000235:genetics / Q000302:isolation & purification*; D005091:Exons; D005136:Eye Proteins; D051597:Hippocalcin; D006801:Humans; D007438:Introns; D008074:Lipoproteins; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000737:chemistry; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D051603:Recoverin; D012160:Retina / Q000737:chemistry*; D012689:Sequence Homology, Nucleic Acid
D002135:Calcium-Binding Proteins; D005136:Eye Proteins; D008074:Lipoproteins; D009419:Nerve Tissue Proteins; C496731:RCVRN protein, human; C045140:visinin; D051603:Recoverin; D051597:Hippocalcin; D004247:DNA; D002118:Calcium