Bos1p, a membrane protein required for ER to Golgi transport in yeast, co-purifies with the carrier vesicles and with Bet1p and the ER membrane.
BOS1 and BET1 are required for transport from the ER to the Golgi complex in yeast and genetically interact with each other and a subset of the other genes, whose products function at this stage of the secretory pathway. In a previous study, we reported that BOS1 encodes a putative 27 kDa membrane protein. Here we show that BET1 is structurally similar to the synaptobrevins and identical to the SLY12 gene product. Overexpression of SLY12 compensates for the loss of function of the ras-like GTP-binding protein Ypt1. Both Bos1p and Bet1p are cytoplasmically oriented membrane proteins. Bos1p co-purifies with the ER to Golgi transport vesicles and co-fractionates with Bet1p and the ER membrane.
Newman
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Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06510.
Groesch
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Ferro-Novick
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D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D002499:Centrifugation, Density Gradient; D004271:DNA, Fungal / Q000235:genetics / Q000302:isolation & purification; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D005800:Genes, Fungal; D016153:Genes, Suppressor; D006056:Golgi Apparatus / Q000378:metabolism*; D008565:Membrane Proteins / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D016133:Polymerase Chain Reaction; D050766:Qb-SNARE Proteins; D012331:RNA, Fungal / Q000235:genetics / Q000302:isolation & purification; D011993:Recombinant Fusion Proteins / Q000276:immunology / Q000302:isolation & purification; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins; D033921:Vesicular Transport Proteins
C068037:BOS1 protein, S cerevisiae; D002352:Carrier Proteins; D004271:DNA, Fungal; D008565:Membrane Proteins; D009838:Oligodeoxyribonucleotides; D050766:Qb-SNARE Proteins; D012331:RNA, Fungal; D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins; D033921:Vesicular Transport Proteins