title: Effect of nanoparticle-bound ampicillin on the survival of Listeria monocytogenes in mouse peritoneal macrophages.
abstract: The efficacy of ampicillin bound to polyisohexylcyanoacrylate nanoparticles was studied in vitro in mouse peritoneal macrophages infected with Listeria monocytogenes. Nanoparticles containing ampicillin 1 mg/L were more effective after 30 h than free ampicillin at the same concentration, with viable counts of 3.68 and 5.43 log10 cfu/mL, respectively. The nanoparticles acted on the intracellular bacteria after a lag period of 6-9 h; this time was apparently required for the degradation of the polymer. At the doses used in these experiments, empty nanoparticles had neither an anti-listeria nor a cytotoxic effect.
authors.affiliation: Département de Microbiologìe et Immunologie, Faculté de Pharmacie, Université Paris XI, Châtenay-Malabry, France.
authors.forename: F
authors.initials: F
authors.lastname: Forestier
authors.forename: P
authors.initials: P
authors.lastname: Gerrier
authors.forename: C
authors.initials: C
authors.lastname: Chaumard
authors.forename: A M
authors.initials: AM
authors.lastname: Quero
authors.forename: P
authors.initials: P
authors.lastname: Couvreur
authors.forename: C
authors.initials: C
authors.lastname: Labarre
mesh_terms: D000667:Ampicillin / Q000494:pharmacology*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D005260:Female; D066298:In Vitro Techniques; D008089:Listeria monocytogenes / Q000187:drug effects*; D008264:Macrophages / Q000187:drug effects / Q000382:microbiology*; D051379:Mice; D008863:Microspheres
chemical_list: D000900:Anti-Bacterial Agents; D000667:Ampicillin