title: Effect of cobalt-60 irradiation on the infectivity of Paragonimus westermani metacercariae.
abstract: A study was made to observe the effect of cobalt-60 irradiation on the viability of Paragonimus westermani metacercariae in Sinopotamon chekiangense crabs. The crabs were collected in mountain regions of the Zhejiang Province of China in which paragonimiasis is endemic. Adult cats and albino mice were infected with metacercariae irradiated at different doses. Dissection of the host animals was conducted 90 or 30 days, respectively, after infection for recovery of lung flukes. Anti-metacercariae antibody in infected mice was measured by enzyme-linked immunosorbent assay (ELISA). Results showed that metacercariae were unable to grow into adult worms in cats after exposure to gamma irradiation at a dose of 0.10 kGray. However, a small number of metacercariae exposed to a dose of 2.0 kGray excysted and survived in 1 mouse for 30 days. No worm was recovered from mice when the metacercariae were irradiated at a dose of 2.5 kGray. Seropositive results by ELISA were obtained when the mice were infected with metacercariae irradiated at doses ranging from 2.0 to 3.5 kGray.
authors.affiliation: Institute of Parasitic Diseases, Zhejiang Academy of Medical Sciences, Hangzhou, People's Republic of China.
authors.forename: C C
authors.initials: CC
authors.lastname: Song
authors.forename: Y F
authors.initials: YF
authors.lastname: Duan
authors.forename: G C
authors.initials: GC
authors.lastname: Shou
authors.forename: H
authors.initials: H
authors.lastname: Zhu
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D003386:Brachyura / Q000469:parasitology; D002415:Cats; D003037:Cobalt Radioisotopes; D004199:Disease Vectors; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000469:parasitology; D005720:Gamma Rays; D008168:Lung / Q000469:parasitology; D008297:Male; D051379:Mice; D010237:Paragonimiasis / Q000469:parasitology*; D010238:Paragonimus / Q000276:immunology / Q000502:physiology / Q000528:radiation effects*
chemical_list: D000909:Antibodies, Helminth; D003037:Cobalt Radioisotopes