Analysis of the specificity of the salivary antigens of Ornithodoros erraticus for the purpose of serological detection of swine farms harbouring the parasite.
In Spain, considerable efforts are currently being devoted to the eradication of Ornithodoros erraticus from the swine farms harbouring this parasite, the European vector of African swine fever (ASF). However, to do so, a preliminary requirement is to determine on which farms it is present. Of all possible methods for discovering this, the only one feasible for large scale application is the serological detection of swine bearing anti-O. erraticus antibodies. To apply serology it was necessary to check the specificity of extracts from the salivary glands (SGE) from O. erraticus. For this, indirect ELISA, competitive ELISA and Western blot were used to assay the SGE from O. erraticus and their corresponding antisera against the SGE and respective antisera from 4 ixodidae, one mange mite, one louse and a mosquito. The results obtained show that only the anti-ixodidae sera are able to react against the SGE from O. erraticus. The cause of this reaction are the somatic antigens present in the SGE of the argasid but not its soluble (secretory) antigens. It is proposed that the anti-cement antibodies present in the anti-ixodidae sera are those that react with the somatic antigens of O. erraticus.
Pérez-Sánchez
R
R
Department of Parisitology, Faculty of Pharmacy, Salamanca University, Spain.
Oleaga-Pérez
A
A
Encinas-Grandes
A
A
D000358:African Swine Fever Virus; D000818:Animals; D001667:Binding, Competitive / Q000276:immunology; D015153:Blotting, Western / Q000662:veterinary; D003429:Cross Reactions / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D000939:Epitopes / Q000276:immunology*; D005260:Female; D008297:Male; D012471:Salivary Proteins and Peptides / Q000276:immunology*; D013552:Swine; D013984:Tick Infestations / Q000276:immunology / Q000662:veterinary*; D013987:Ticks / Q000276:immunology*
D000939:Epitopes; D012471:Salivary Proteins and Peptides