PCR detection of the rat brain basic fibroblast growth factor (bFGF) mRNA containing a unique 3' untranslated region.
We utilized the reverse transcription-polymerase chain reaction (RT-PCR) to detect the presence of basic fibroblast growth factor (bFGF) messenger RNA in rat brain, ovary and kidney. The nucleotide sequence of the RT-PCR product revealed a novel 3' untranslated (UT) sequence in the rat basic FGF mRNA. In this sequence, as in the 3' UT regions of many growth regulatory genes, there is a high degree of conservation of A+T rich motifs which have been shown to play a major role in mRNA stability.
el-Husseini
A E
AE
Department of Physiology, Faculty of Medicine, University of Manitoba, Winnipeg, Canada.
Paterson
J A
JA
Myal
Y
Y
Shiu
R P
RP
D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D001923:Brain Chemistry; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000032:analysis / Q000235:genetics*; D007668:Kidney / Q000737:chemistry; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000235:genetics; D010053:Ovary / Q000737:chemistry; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics* / Q000378:metabolism; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D051381:Rats
D009838:Oligodeoxyribonucleotides; D012333:RNA, Messenger; D016222:Fibroblast Growth Factor 2; D012194:RNA-Directed DNA Polymerase