title: Sequence analysis of the 3'-terminal halves of RNA 1 of two strains of barley mild mosaic virus.
abstract: DNA complementary to the 3'-terminal halves of RNA 1 of two strains of barley mild mosaic virus (BaMMV) from Japan, BaMMV-Ka1 and BaMMV-Na1, was cloned and sequenced. The sequences start within a single long open reading frame (ORF), and are followed by 337 and 338 3' non-coding nucleotides, for BaMMV-Ka1 and BaMMV-Na1 respectively. The two strains have 88% nucleotide identity in the ORFs and 92% identity in the non-coding regions. The putative ORF products contain the capsid proteins at the C termini, as indicated by amino acid sequence analysis, and two putative non-structural proteins are arranged in the same manner as in RNA 1 of barley yellow mosaic virus (BaYMV). The deduced capsid proteins of BaMMV-Ka1 and BaMMV-Na1 each contain 251 amino acids and have 94% sequence identity, which is compatible with their close serological relationship. Most of the sequence differences between the two capsid proteins are found in the N-terminal region, and might explain their serological differences. Significant sequence similarities of the capsid proteins of the two BaMMV strains (37 and 35% respectively) with that of BaYMV, and their marginal similarities (21 to 26%) to the capsid proteins of aphid-borne or mite-borne potyviruses support the classification of BaMMV and BaYMV as distinct members of the same virus group, which is separate from the group(s) containing aphidborne or mite-borne potyviruses.
authors.affiliation: National Agriculture Research Center, Ibaraki, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Kashiwazaki
authors.forename: K
authors.initials: K
authors.lastname: Nomura
authors.forename: H
authors.initials: H
authors.lastname: Kuroda
authors.forename: K
authors.initials: K
authors.lastname: Ito
authors.forename: H
authors.initials: H
authors.lastname: Hibino
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002213:Capsid / Q000235:genetics; D003001:Cloning, Molecular; D004277:DNA, Single-Stranded / Q000235:genetics; D005814:Genes, Viral / Q000235:genetics*; D001467:Hordeum / Q000382:microbiology*; D008969:Molecular Sequence Data; D009029:Mosaic Viruses / Q000145:classification / Q000235:genetics*; D016366:Open Reading Frames; D011506:Proteins / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D014764:Viral Proteins / Q000235:genetics*; D015678:Viral Structural Proteins / Q000235:genetics*
chemical_list: D004277:DNA, Single-Stranded; D011506:Proteins; D012367:RNA, Viral; D014764:Viral Proteins; D015678:Viral Structural Proteins