title: Rescue and expression of human immunoglobulin genes to generate functional human monoclonal antibodies.
abstract: Human monoclonal antibody production has been hampered for many years by the instability of cell lines and low levels of expression of the antibodies. We describe here the rescue of human immunoglobulin genes utilizing micro-mRNA preparation from a small number of human hybridoma cells and conventional cDNA cloning. This allows cloning and immediate high-level expression from full-length human heavy and light chain cDNA molecules and provides a mechanism to rescue whole human monoclonal antibodies of proven efficacy.
authors.affiliation: Department of Cell Biology, Wellcome Research Laboratories, Beckenham, Kent, U.K.
authors.forename: A P
authors.initials: AP
authors.lastname: Lewis
authors.forename: N
authors.initials: N
authors.lastname: Parry
authors.forename: T C
authors.initials: TC
authors.lastname: Peakman
authors.forename: J S
authors.initials: JS
authors.lastname: Crowe
mesh_terms: D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D015870:Gene Expression; D005803:Genes, Immunoglobulin; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics
chemical_list: D000911:Antibodies, Monoclonal; D012333:RNA, Messenger; D004247:DNA