title: Flow cytometry: an overview.
abstract: Flow cytometry is a method of rapidly analyzing large numbers of cells as they flow in a liquid medium through a laser source. Flow cytometry provides valuable information regarding DNA content, RNA content, and the percentage of cells in the S phase of the cell cycle. This information can be used to predict the aggressiveness of many tumors, which has direct prognostic and diagnostic implications. Flow cytometry assists healthcare professionals in planning and implementing effective, appropriate, and timely interventions. Because flow cytometry is becoming more prevalent, nurses must be aware of its actual and potential applications. The data obtained from flow cytometry provide important information that can assist nurses in anticipating a particular regimen for specific tumors and in projecting the patient's clinical course. With this information, nurses can modify and individualize care plans. When used in conjunction with a patient's clinical presentation and other laboratory findings, flow cytometry has a direct impact on both treatment decisions and nursing interventions.
authors.affiliation: Pittsburgh Cancer Institute, PA.
authors.forename: M L
authors.initials: ML
authors.lastname: Madeya
authors.forename: J M
authors.initials: JM
authors.lastname: Pfab-Tokarsky
mesh_terms: D004247:DNA / Q000032:analysis; D005434:Flow Cytometry / Q000295:instrumentation / Q000379:methods* / Q000451:nursing; D006801:Humans; D016131:Lymphocyte Subsets; D009859:Oncology Nursing; D011786:Quality Control; D012313:RNA / Q000032:analysis; D016196:S Phase
chemical_list: D012313:RNA; D004247:DNA