6-Dimethyl amino purine and 2-amino purine inhibit the induction of expression of milk protein genes by prolactin.
Two protein kinase-inhibitors, 6-dimethyl amino purine and 2-amino purine inhibited induction of beta-casein synthesis by prolactin when added to the culture medium of rabbit mammary explant and cells. The accumulation of the mRNA for alpha s1- and beta-caseins and for whey acidic protein did not take place in the presence of the inhibitors whereas beta-actin mRNA concentration was not altered. In the same experimental conditions, H7, an inhibitor of protein kinase C and, to a lower extent, of protein kinase A did not prevent prolactin from acting. These data suggest for the first time that specific protein kinases are involved in the transduction of the prolactin signal to milk protein genes.
Bayat-Sarmadi
M
M
Unité de Différenciation Cellulaire, Institut National de la Recherche Agronomique, Jouy-en-Josas, France.
Maliénou-Ngassa
R
R
Puissant
C
C
Houdebine
L M
LM
D015075:2-Aminopurine / Q000494:pharmacology*; D000225:Adenine / Q000031:analogs & derivatives* / Q000494:pharmacology; D000818:Animals; D002364:Caseins / Q000235:genetics; D002478:Cells, Cultured; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D008321:Mammary Glands, Animal / Q000187:drug effects / Q000378:metabolism; D008894:Milk Proteins / Q000235:genetics*; D011388:Prolactin / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011817:Rabbits
D002364:Caseins; D008894:Milk Proteins; D012333:RNA, Messenger; C024471:whey acidic proteins; D015075:2-Aminopurine; C001020:N(6),N(6)-dimethyladenine; D011388:Prolactin; D000225:Adenine