title: Cloning and sequencing of the cDNA encoding rice elongation factor 1 beta'.
abstract: A cDNA clone coding for rice elongation factor 1 beta' (EF-1 beta') was isolated from a rice anther cDNA library. The clone, named RB', was 980 bp long and contained a single open reading frame coding for 223 amino acids; the first 31 amino acids, except for the first methionine, which is absent in the mature protein, are identical to those of the purified protein determined with a protein sequencer. The amino acid sequence of rice EF-1 beta' shows homology to the C-terminal half of Artemia salina EF-1 beta (59%) and human EF-1 beta (63%), but might not have a phosphorylation site for casein kinase II which has been conserved in Artemia saline EF-1 beta and EF-1 delta, human EF-1 beta and silkworm EF-1 beta'.
authors.affiliation: Institute for Cell Biology and Genetics, Faculty of Agriculture, Iwate University, Morioka, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Matsumoto
authors.forename: N
authors.initials: N
authors.lastname: Oizumi
authors.forename: H
authors.initials: H
authors.lastname: Taira
authors.forename: S
authors.initials: S
authors.lastname: Ejiri
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004277:DNA, Single-Stranded / Q000235:genetics; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D012275:Oryza / Q000235:genetics*; D020648:Peptide Elongation Factor 1; D010445:Peptide Elongation Factors / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
chemical_list: D004277:DNA, Single-Stranded; D020648:Peptide Elongation Factor 1; D010445:Peptide Elongation Factors; D010940:Plant Proteins