Description of a variably expressed and labile epitope of thyroglobulin and its occurrence in different thyroid diseases using a monoclonal antibody.
A new category of epitopes of human thyroglobulin is being described defined by a monoclonal antibody (TuTg 1). The epitope shows a very variable expression in normal individuals as well as in different thyroid diseases. According to gel filtration data the epitope is located at the intact molecule and is very sensitive to tryptic digestion. The monoclonal antibody was used in an IRMA system for plasma measurements in the follow-up of thyroid cancer patients. In individuals with high epitope expression the sensitivity to detect recurrency during T4 treatment was higher than a sensitive commercial TG IRMA.
Hüfner
M
M
Department of Internal Medicine V, University of Heidelberg, Germany.
Kiehne
B
B
Stempka
L
L
Bethäuser
H
H
Schulz
R
R
D000911:Antibodies, Monoclonal / Q000276:immunology*; D002850:Chromatography, Gel; D000939:Epitopes; D006801:Humans; D013954:Thyroglobulin / Q000096:biosynthesis / Q000276:immunology*; D013964:Thyroid Neoplasms / Q000276:immunology / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D000939:Epitopes; D013954:Thyroglobulin