Profile of renal permselectivity by simultaneous enzyme-linked immunosorbent assay of albumin, transferrin, IgG, and alpha 1-microglobulin with a new microplate reader.
A competitive enzyme-linked immunosorbent assay (ELISA) is described for determining a renal permselectivity profile involving the urinary proteins albumin, transferrin, IgG, and alpha 1-microglobulin (alpha 1-m). The ELISA reader uses a computer-controlled array of multiplexed light-emitting diode (LED)-photodiode pairs for rapid measurements of absorbance on microplates. A 3,3'-dimethylnaphthidine reagent adapts the 3,5,3',5'-tetramethylbenzidine chromophore to monochromatic LED emission at 550 nm. We applied this ELISA to the determination of renal permselectivity in healthy children and young adults and in children with insulin-dependent diabetes mellitus. The geometric means (and SD) of protein excretion rates in a group of 85 normal subjects were as follows: albumin, 3.5 micrograms/min (1.83); transferrin, 173 ng/min (2.76); IgG, 1.11 micrograms/min (2.22), and alpha 1-m, 0.98 microgram/min (2.36).
Magnotti
R A
RA
Department of Pediatrics, University of Cincinnati, OH 45267-0585.
Eberly
J P
JP
Khoury
P R
PR
Daniels
S R
SR
Drozda
D J
DJ
Turner
A M
AM
Dolan
L M
LM
D000293:Adolescent; D000328:Adult; D000419:Albuminuria / Q000652:urine*; D000510:Alpha-Globulins / Q000652:urine*; D002648:Child; D002675:Child, Preschool; D003922:Diabetes Mellitus, Type 1 / Q000652:urine*; D003928:Diabetic Nephropathies / Q000652:urine*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods* / Q000706:statistics & numerical data; D006801:Humans; D007074:Immunoglobulin G / Q000652:urine*; D007202:Indicators and Reagents; D014168:Transferrin / Q000652:urine*
D000510:Alpha-Globulins; D007074:Immunoglobulin G; D007202:Indicators and Reagents; D014168:Transferrin; C016001:alpha-1-microglobulin