title: Rapid morphological changes in bovine brain microvascular endothelial cells on extracellular matrices.
abstract: The morphological changes in endothelial cells derived from bovine brain microvasculature, carotid artery, and aorta during growth on extracellular matrices were compared. All cells formed tubular structures on a basement membrane. Ultrastructural studies showed that the tubular structures had lumens surrounded by many endothelial cells. On type I collagen gel, brain microvascular endothelial cells still formed tubular structures, but the other two cell types formed confluent monolayers. However, when a second layer of collagen gel was laid over these cells, tubular structures developed within 2-3 days. Brain microvascular endothelial cells form tubular structures more readily than endothelial cells derived from large vessels on both basement membrane and type I collagen gel.
authors.affiliation: Department of Neurosurgery, Faculty of Medicine, Kyoto University, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Kaneko
authors.forename: I
authors.initials: I
authors.lastname: Nagata
authors.forename: S
authors.initials: S
authors.lastname: Miyamoto
authors.forename: H
authors.initials: H
authors.lastname: Kubo
authors.forename: H
authors.initials: H
authors.lastname: Kikuchi
authors.forename: T
authors.initials: T
authors.lastname: Fujisato
authors.forename: Y
authors.initials: Y
authors.lastname: Ikada
mesh_terms: D000818:Animals; D001921:Brain / Q000098:blood supply*; D002417:Cattle; D004730:Endothelium, Vascular / Q000473:pathology* / Q000648:ultrastructure; D005109:Extracellular Matrix / Q000473:pathology* / Q000648:ultrastructure; D008833:Microcirculation / Q000473:pathology / Q000648:ultrastructure; D008854:Microscopy, Electron; D009389:Neovascularization, Pathologic / Q000473:pathology