title: Subunits asymmetry in the ternary complex of lamb liver 6-phosphogluconate dehydrogenase detected by a NADP analogue.
abstract: Incubation of lamb liver 6-phosphogluconate dehydrogenase, a dimeric enzyme with periodate-oxidized NADP causes the inactivation of the enzyme due to the covalent binding of 2 mol of inhibitor/mol of dimer. In the presence of substrate, the inactivation is faster and is almost complete after the labelling of only one subunit. These results not only confirm the hypothesis of a 'half-of-the-sites' mechanism of action of the enzyme, but also suggest that the formation of the ternary complex (enzyme-substrate-coenzyme) in one subunit causes a conformational change that makes the other subunit unable to bind the coenzyme (and even the adenylic part of it) and, thus, this subunit becomes inactive. It appears that while one subunit catalyses the oxidation of 6-phosphogluconate the other is inactive in this reaction.
authors.affiliation: Istituto di Chimica Biologica, Universita Ferrara, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Hanau
authors.forename: F
authors.initials: F
authors.lastname: Dallocchio
authors.forename: M
authors.initials: M
authors.lastname: Rippa
mesh_terms: D000818:Animals; D001665:Binding Sites; D003545:Cysteine; D005942:Gluconates / Q000378:metabolism; D008099:Liver / Q000201:enzymology*; D008239:Lysine; D009249:NADP / Q000031:analogs & derivatives / Q000494:pharmacology*; D010734:Phosphogluconate Dehydrogenase / Q000737:chemistry* / Q000187:drug effects; D011487:Protein Conformation; D012756:Sheep
chemical_list: D005942:Gluconates; D009249:NADP; D010734:Phosphogluconate Dehydrogenase; D008239:Lysine; D003545:Cysteine; C008884:6-phosphogluconic acid