title: Covalent linkage of ruthenium polypyridyl compounds to poly(L-lysine), albumins, and immunoglobulin G.
abstract: A series of ruthenium polypyridyl complexes has been covalently bound to poly(L-lysine), bovine serum albumin, human serum albumin, ovalbumin, and immunoglobulin G using different binding methods. The conjugation ratios and the luminescence properties of the bioconjugates are reported. All conjugates show nonsingle-exponential decay curves. Quenching of the emission by oxygen has been studied.
authors.affiliation: School of Chemical Sciences, Dublin City University, Ireland.
authors.forename: E M
authors.initials: EM
authors.lastname: Ryan
authors.forename: R
authors.initials: R
authors.lastname: O'Kennedy
authors.forename: M M
authors.initials: MM
authors.lastname: Feeney
authors.forename: J M
authors.initials: JM
authors.lastname: Kelly
authors.forename: J G
authors.initials: JG
authors.lastname: Vos
mesh_terms: D000418:Albumins / Q000737:chemistry*; D000818:Animals; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002851:Chromatography, High Pressure Liquid; D004797:Enzyme-Linked Immunosorbent Assay; D005456:Fluorescent Dyes / Q000737:chemistry*; D007074:Immunoglobulin G / Q000737:chemistry*; D007202:Indicators and Reagents; D051379:Mice; D009942:Organometallic Compounds / Q000737:chemistry*; D010047:Ovalbumin / Q000737:chemistry / Q000276:immunology; D011107:Polylysine / Q000737:chemistry*; D011725:Pyridines / Q000737:chemistry*; D012428:Ruthenium / Q000737:chemistry; D012709:Serum Albumin / Q000737:chemistry / Q000276:immunology; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000276:immunology
chemical_list: D000418:Albumins; D005456:Fluorescent Dyes; D007074:Immunoglobulin G; D007202:Indicators and Reagents; D009942:Organometallic Compounds; D011725:Pyridines; D012709:Serum Albumin; D011107:Polylysine; D012710:Serum Albumin, Bovine; D012428:Ruthenium; D010047:Ovalbumin