title: Pancreatic secretion of lysosomal enzymes stimulated by intraduodenal instillation of a liquid meal in rabbits.
abstract: 1. Studies have been performed to determine the effect of intraduodenal food on pancreatic secretion of lysosomal enzymes. 2. Intraduodenal instillation of a liquid meal (3 g/kg body weight; 15.3% protein, 19.7% fat, 59.7% carbohydrate) caused significant increases in pancreatic juice volume and pancreatic secretion of amylase and protein compared with basal values for 2h after instillation in anaesthetized rabbits. 3. Intraduodenal instillation of a liquid meal also caused significant increases in pancreatic secretion of three lysosomal enzymes (cathepsin B, N-beta-acetyl-galactosaminidase and N-acetyl-beta-glucosaminidase) compared with basal values for 2h after instillation. 4. In addition, there were significant correlations between cathepsin B secretion and amylase secretion (r = 0.7764, P < 0.001) and between cathepsin B secretion and protein secretion (r = 0.6216, P < 0.001), both in basal conditions and in response to the liquid meal. 5. These results are evidence for the localization of lysosomal enzymes in the secretory granules-zymogen granules in normal acinar cells, and also indicate that the pancreatic secretion of lysosomal enzymes is gut-hormone-regulated.
authors.affiliation: First Department of Surgery, Faculty of Medicine, Kyoto University, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Hirano
authors.forename: T
authors.initials: T
authors.lastname: Manabe
authors.forename: A K
authors.initials: AK
authors.lastname: Saluja
authors.forename: M L
authors.initials: ML
authors.lastname: Steer
mesh_terms: D000118:Acetylglucosaminidase / Q000032:analysis; D000681:Amylases / Q000032:analysis; D000818:Animals; D002401:Cathepsin B / Q000032:analysis; D004386:Duodenum / Q000378:metabolism; D004750:Enteral Nutrition; D006596:Hexosaminidases / Q000032:analysis; D008247:Lysosomes / Q000201:enzymology*; D010179:Pancreas / Q000201:enzymology*; D010189:Pancreatic Juice / Q000737:chemistry / Q000201:enzymology*; D011506:Proteins / Q000032:analysis; D011817:Rabbits; D048828:beta-N-Acetyl-Galactosaminidase
chemical_list: D011506:Proteins; D000681:Amylases; D006596:Hexosaminidases; D000118:Acetylglucosaminidase; D048828:beta-N-Acetyl-Galactosaminidase; D002401:Cathepsin B