title: Use of in vitro-transcribed RNAs as immobilized targets during RNA:RNA hybridization.
abstract: We describe the use of in vitro-transcribed complementary RNAs as filter-bound targets during nuclear run-on analyses. Use of these single-stranded reagents in high-stringency RNA:RNA hybridizations increases signal-to-background hybridization seen using DNA targets and allows efficient measurement of transcriptional rates across genes in either direction.
authors.affiliation: Department of Molecular Genetics and Biochemistry, Univ. of Pittsburgh Sch. of Med., PA 15261-2072.
authors.forename: J A
authors.initials: JA
authors.lastname: Flaspohler
authors.forename: C
authors.initials: C
authors.lastname: Milcarek
mesh_terms: D000818:Animals; D005821:Genetic Techniques; D008566:Membranes; D051379:Mice; D009693:Nucleic Acid Hybridization; D012313:RNA / Q000032:analysis* / Q000235:genetics; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured
chemical_list: D012313:RNA