title: Direct immunofluorescence of oral mucosal biopsies: a comparison of fresh-frozen tissue and formalin-fixed, paraffin-embedded tissue.
abstract: The diagnosis of some oral mucosal diseases can be facilitated by the use of direct immunofluorescence. Fresh frozen tissue is preferred when using this technique, however frequently the material received by the histopathology laboratory has been fixed in formalin. Enzyme digestion of tissue sections glued to glass slides prior to incubation with antibodies was shown in this study to give comparable results with direct immunofluorescence on fresh frozen tissue and can be used for diagnostic purposes when fresh frozen tissue is unavailable or not representative of the disease process.
authors.affiliation: Section of Oral Medicine and Oral Surgery, School of Dental Science, University of Melbourne, Victoria, Australia.
authors.forename: N A
authors.initials: NA
authors.lastname: Firth
authors.forename: A M
authors.initials: AM
authors.lastname: Rich
authors.forename: B G
authors.initials: BG
authors.lastname: Radden
authors.forename: P C
authors.initials: PC
authors.lastname: Reade
mesh_terms: D001485:Basement Membrane / Q000473:pathology; D003165:Complement System Proteins / Q000032:analysis; D003238:Connective Tissue / Q000473:pathology; D015925:Cryopreservation / Q000379:methods; D004848:Epithelium / Q000473:pathology; D004892:Erythema Multiforme / Q000473:pathology; D005340:Fibrinogen / Q000032:analysis; D005455:Fluorescent Antibody Technique; D005557:Formaldehyde; D006801:Humans; D007136:Immunoglobulins / Q000032:analysis; D007365:Intercellular Junctions / Q000648:ultrastructure; D008010:Lichen Planus / Q000473:pathology; D009059:Mouth Diseases / Q000473:pathology*; D009061:Mouth Mucosa / Q000473:pathology*; D016612:Paraffin Embedding; D010390:Pemphigoid, Benign Mucous Membrane / Q000473:pathology; D010392:Pemphigus / Q000473:pathology; D013194:Staining and Labeling; D016707:Tissue Fixation / Q000379:methods*
chemical_list: D007136:Immunoglobulins; D005557:Formaldehyde; D005340:Fibrinogen; D003165:Complement System Proteins