title: A yeast splicing factor is localized in discrete subnuclear domains.
abstract: Digital imaging microscopy has been used to visualize the splicing protein PRP6p and three other yeast nuclear proteins. The results show that PRP6p is uniquely localized to discrete subnuclear regions. A combination of cytological and biochemical assays suggests that these sites can be saturated when the protein is overexpressed and likely correspond to the location of U4/U6 snRNPs. The observations indicate that some splicing components are located in discrete subregions of the yeast nucleus, similar to the situation described for the mammalian nucleus.
authors.affiliation: Howard Hughes Medical Institute, Waltham, MA.
authors.forename: D J
authors.initials: DJ
authors.lastname: Elliott
authors.forename: D S
authors.initials: DS
authors.lastname: Bowman
authors.forename: N
authors.initials: N
authors.lastname: Abovich
authors.forename: F S
authors.initials: FS
authors.lastname: Fay
authors.forename: M
authors.initials: M
authors.lastname: Rosbash
mesh_terms: D015153:Blotting, Western; D002467:Cell Nucleus / Q000378:metabolism* / Q000648:ultrastructure; D005656:Fungal Proteins / Q000032:analysis / Q000378:metabolism*; D012319:RNA Polymerase II / Q000032:analysis / Q000378:metabolism; D012326:RNA Splicing; D012342:RNA, Small Nuclear / Q000378:metabolism; D017414:Ribonucleoprotein, U4-U6 Small Nuclear; D017415:Ribonucleoprotein, U5 Small Nuclear; D017411:Ribonucleoproteins, Small Nuclear / Q000302:isolation & purification / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D029701:Saccharomyces cerevisiae Proteins
chemical_list: D005656:Fungal Proteins; C065573:PRP6 protein, S cerevisiae; D012342:RNA, Small Nuclear; D017414:Ribonucleoprotein, U4-U6 Small Nuclear; D017415:Ribonucleoprotein, U5 Small Nuclear; D017411:Ribonucleoproteins, Small Nuclear; D029701:Saccharomyces cerevisiae Proteins; D012319:RNA Polymerase II