[The potencies of substance P, substance P fragments, and compound 48/80 for granulocyte infiltration in mouse skin].
To determine whether the N-terminal or C-terminal peptide of substance P (SP) induces granulocyte infiltration in mouse skin, we examined the potencies of SP, the N-terminal peptides SP1-4 and SP1-9, the C-terminal peptides SP4-11 and SP6-11, and a mast cell degranulating agent compound 48/80 in inducing granulocyte (neutrophil and eosinophil) infiltration in the skin of BALB/c mice. The subcutaneous administration of SP (10(-7)-10(-5) M) caused granulocyte infiltration in mouse skin in a concentration-dependent fashion 6 h after the injection. SP1-9 (10(-5)-10(-4) M) also caused granulocyte infiltration in the skin which was associated with mast cell degranulation. However, SP1-4, SP4-11 and SP6-11 (up to 10(-4) M) induced neither granulocyte infiltration nor mast cell degranulation. In addition, compound 48/80 (0.5-50 micrograms/ml) also induced granulocyte infiltration of mouse skin with a concentration-dependent increase in mast cell degranulation. These results indicate that SP induces granulocyte infiltration of mouse skin through mast cell degranulation induced by the N-terminal peptide.
Tomoe
S
S
Second Department of Internal Medicine, Chiba University School of Medicine.
Iwamoto
I
I
Yoshida
S
S
Tomioka
H
H
D000818:Animals; D015550:Cell Degranulation / Q000187:drug effects; D006098:Granulocytes / Q000187:drug effects* / Q000276:immunology; D008297:Male; D008407:Mast Cells / Q000187:drug effects / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C; D010446:Peptide Fragments / Q000494:pharmacology*; D012867:Skin / Q000276:immunology*; D013373:Substance P / Q000494:pharmacology*; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology*
D010446:Peptide Fragments; D013373:Substance P; D003189:p-Methoxy-N-methylphenethylamine