Laser desorption studies of high mass biomolecules in Fourier-transform ion cyclotron resonance mass spectrometry.
Matrix-assisted laser desorption ionization is used to obtain Fourier-transform ion cyclotron resonance mass spectra of model peptides (e.g., gramicidin S, angiotensin I, renin substrate, melittin, and bovine insulin). Matrix-assisted laser desorption ionization yields ions having appreciable kinetic energies. Two methods for trapping the high kinetic energy ions are described: (i) the ion signal for [M+H]+ ions is shown to increase with increasing trapping voltages, and (ii) collisional relaxation is used for the detection of [M+H]+ ions of bovine insulin.
Solouki
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Department of Chemistry, Texas A&M University, College Station 77843.
Russell
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DH
D000803:Angiotensin I / Q000737:chemistry; D000808:Angiotensinogen / Q000737:chemistry; D000818:Animals; D002417:Cattle; D005583:Fourier Analysis; D006096:Gramicidin / Q000737:chemistry; D066298:In Vitro Techniques; D007328:Insulin / Q000737:chemistry; D007834:Lasers; D013058:Mass Spectrometry / Q000295:instrumentation* / Q000379:methods; D008555:Melitten / Q000737:chemistry; D010315:Particle Accelerators; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry*
D007328:Insulin; D011506:Proteins; D000808:Angiotensinogen; D006096:Gramicidin; D008555:Melitten; D000803:Angiotensin I