Functional studies on Calliphora vomitoria haemocyte subpopulations defined by lectin staining and density centrifugation.
Haemocyte subpopulations of Calliphora vomitoria have been categorized by their surface staining properties using fluorescently labelled lectins, and their mobilities in Percoll density gradients. These methods of identification were exploited to determine the roles of these cell types in cellular defence reactions. Soybean agglutinin clearly defined the cell subpopulation involved in phagocytosis, while purified thrombocytoid fragments proved to be the main haemocyte population involved in encapsulation and nodule formation.
McKenzie
A N
AN
Department of Biology, University College London, U.K.
Preston
T M
TM
D000818:Animals; D015415:Biomarkers; D002469:Cell Separation / Q000379:methods*; D002499:Centrifugation, Density Gradient; D004175:Diptera / Q000276:immunology*; D006434:Hemocytes / Q000502:physiology*; D007211:Indoles; D007814:Larva; D037102:Lectins; D008562:Membrane Glycoproteins / Q000032:analysis; D010587:Phagocytosis; D011205:Povidone; D012822:Silicon Dioxide; D013194:Staining and Labeling
D015415:Biomarkers; D007211:Indoles; D037102:Lectins; D008562:Membrane Glycoproteins; C007293:DAPI; C016039:Percoll; D012822:Silicon Dioxide; D011205:Povidone