Effect of a high concentration perfluorocarbon emulsion on platelet function.
Perfluorocarbon (PFC) and lipid emulsions (eg. Fluosol, Intralipid,) containing phospholipid have been reported to modify platelet function after intravenous infusion. Platelet activation might be responsible for the generation of mediators responsible for PFC-induced side effects. In view of this, we investigated the effect of a highly concentrated perfluorocarbon emulsion containing 90% (w/v) perfluorooctylbromide (perflubron; PFOB), on porcine and human platelet activation and function. We measured both: a) stimulated ex-vivo porcine platelet aggregation pre and post infusion of either perflubron or control (vehicle only) emulsions, and b) stimulated in vitro human platelet calcium flux in the presence of perflubron emulsion or control emulsions. Platelet aggregation stimulated by collagen, ADP or arachidonic acid (AA) was inhibited in ex-vivo porcine platelets following infusion of perflubron emulsion at a dose of 3 ml/kg (0.2 ml/kg/min). Inhibition was dose dependent and was decreased when the dose of perflubron emulsion was reduced to 1.0 or 0.3 ml/kg. Infusion of saline or "vehicle" emulsions had little or no effect on stimulated ex-vivo pig platelet aggregation. Fluosol, infusion was associated with inconsistent inhibition of platelet aggregation. A23187- or AA-stimulated calcium flux of human platelets in vitro was inhibited in the presence of 1% (v/v) perflubron emulsion. Similar effects were seen with Fluosol, or Intralipid. This inhibition was agonist dose-dependent.(ABSTRACT TRUNCATED AT 250 WORDS)
Smith
D J
DJ
Alliance Pharmaceutical Corporation, San Diego, CA.
Lane
T A
TA
D000818:Animals; D001792:Blood Platelets / Q000187:drug effects* / Q000378:metabolism; D001802:Blood Substitutes / Q000633:toxicity*; D002118:Calcium / Q000097:blood; D004655:Emulsions; D005217:Fat Emulsions, Intravenous / Q000633:toxicity; D005466:Fluorocarbons / Q000633:toxicity*; D006801:Humans; D006842:Hydrocarbons, Brominated; D066298:In Vitro Techniques; D010974:Platelet Aggregation / Q000187:drug effects; D013552:Swine
D001802:Blood Substitutes; D004655:Emulsions; D005217:Fat Emulsions, Intravenous; D005466:Fluorocarbons; D006842:Hydrocarbons, Brominated; C050455:fluosol; C003072:perflubron; D002118:Calcium