Interleukin-2 (IL-2) induces erythroid differentiation and tyrosine phosphorylation in ELM-I-1 cells transfected with a human IL-2 receptor beta chain cDNA.
The molecular mechanism of erythroid differentiation has been still ill-defined. In this study, we introduced a human interleukin-2 receptor (IL-2R) beta chain cDNA into ELM-I-1 cells which differentiated into hemoglobin-positive cells in the presence of erythropoietin (Epo), and established the transformant which expressed IL-2R beta chain. In this transformant, we revealed that IL-2 induced erythroid differentiation and the same pattern of tyrosine phosphorylation as Epo. These data suggest that tyrosine phosphorylation is involved in signal transduction pathway of erythroid differentiation. It is also implicated that the Epo and IL-2 receptor system share a common signal transduction pathway.
Imada
K
K
Institute for Virus Research, Kyoto University, Japan.
Tsudo
M
M
Kodaka
T
T
Itoh
K
K
Arima
N
N
Hattori
T
T
Okuma
M
M
Uchiyama
T
T
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D004921:Erythropoietin / Q000494:pharmacology; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology*; D007700:Kinetics; D004915:Leukemia, Erythroblastic, Acute; D046911:Macromolecular Substances; D051379:Mice; D008970:Molecular Weight; D010750:Phosphoproteins / Q000302:isolation & purification / Q000378:metabolism; D010766:Phosphorylation; D019000:Phosphotyrosine; D015375:Receptors, Interleukin-2 / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D011994:Recombinant Proteins / Q000494:pharmacology; D014162:Transfection; D014407:Tumor Cells, Cultured; D014443:Tyrosine / Q000031:analogs & derivatives / Q000032:analysis
D007376:Interleukin-2; D046911:Macromolecular Substances; D010750:Phosphoproteins; D015375:Receptors, Interleukin-2; D011994:Recombinant Proteins; D004921:Erythropoietin; D019000:Phosphotyrosine; D014443:Tyrosine