Characterization of a class 3 tyrosine kinase.
In an effort to identify unique tyrosine kinases found in human leukemia cell lines, we utilized polymerase chain reaction (PCR) technology and degenerate oligonucleotide primers to produce a cDNA library of kinase catalytic domains found in the human monocytic cell line AML-193. This search yielded a member of the class 3 tyrosine kinases closely related to the murine kinase FD-22. Previous work has identified this kinase as JAK1. This class of tyrosine kinases is characterized by being ubiquitously expressed, lacking both a ligand-binding domain and a SH2 domain, while containing a second domain similar to a degenerate kinase domain. Our studies focused on the further characterization of this class 3 tyrosine kinase using Northern blot analysis to demonstrate an increase in steady-state mRNA by interferon-gamma in human monocytes. A human-hamster somatic cell hybrid panel and linkage mapping was used to assign JAK1 (aml-116) to human chromosome 1.
Howard
O M
OM
Biological Carcinogenesis and Development Program, Program Resources, Inc/DynCorp, Frederick, Maryland 21702.
Dean
M
M
Young
H
H
Ramsburg
M
M
Turpin
J A
JA
Michiel
D F
DF
Kelvin
D J
DJ
Lee
L
L
Farrar
W L
WL
D000483:Alleles; D000595:Amino Acid Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D002874:Chromosome Mapping; D002878:Chromosomes, Human, Pair 1; D005787:Gene Frequency; D015723:Gene Library; D006801:Humans; D006822:Hybrid Cells; D007948:Leukemia, Monocytic, Acute / Q000201:enzymology; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D011505:Protein-Tyrosine Kinases / Q000235:genetics*; D012313:RNA / Q000032:analysis; D012689:Sequence Homology, Nucleic Acid; D014407:Tumor Cells, Cultured
D012313:RNA; D011505:Protein-Tyrosine Kinases