title: Mucin histochemistry of pancreatic duct cell carcinoma, with special reference to organoid differentiation simulating gastric pyloric mucosa.
abstract: The present study was undertaken to explore mucins produced in normal, metaplastic, and hyperplastic ductal epithelia as well as in carcinoma tissues of the pancreas, and used a battery of histochemical techniques. Thirty-five cases of ordinary pancreatic duct cell carcinoma and nine cases of duct cell carcinoma, which fulfilled the clinical criteria of "mucin-producing carcinoma of the pancreas," were examined. The results indicated that all lesions of mucinous metaplasia with or without papillary hyperplasia as well as atypical hyperplasia characteristically had gastric mucins and showed organoid differentiation simulating the gastric pyloric mucosa, but never stained for 8-O-acetyl-N-acetylneuraminic acid, which is a histochemical marker of the large intestine. Ordinary duct cell carcinoma also contained gastric mucins and small intestinal mucins and showed organoid differentiation, but rarely had 8-O-acetyl-N-acetylneuraminic acid. Mucin-producing carcinomas were classified into two groups: the ordinary duct cell carcinoma group and a group that showed marked atrophy and extensive fibrosis of the parenchyma, a lack of organoid differentiation and gastric mucins, and an abundance of large and small intestinal mucins. These results suggest that gastrointestinal mucins are useful markers to detect cancer-related lesions and cancers of the pancreas.
authors.affiliation: Second Department of Internal Medicine, Shinshu University School of Medicine, Matsumoto, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Matsuzawa
authors.forename: T
authors.initials: T
authors.lastname: Akamatsu
authors.forename: T
authors.initials: T
authors.lastname: Katsuyama
mesh_terms: D000328:Adult; D000368:Aged; D001284:Atrophy; D014408:Biomarkers, Tumor / Q000032:analysis; D002285:Carcinoma, Intraductal, Noninfiltrating / Q000737:chemistry* / Q000473:pathology* / Q000648:ultrastructure; D002454:Cell Differentiation / Q000502:physiology; D004848:Epithelium / Q000737:chemistry / Q000473:pathology / Q000648:ultrastructure; D005260:Female; D005355:Fibrosis; D005752:Gastric Mucins / Q000032:analysis*; D005753:Gastric Mucosa / Q000473:pathology* / Q000648:ultrastructure; D006651:Histocytochemistry; D006801:Humans; D008297:Male; D008875:Middle Aged; D009940:Organoids / Q000473:pathology*; D010183:Pancreatic Ducts; D010190:Pancreatic Neoplasms / Q000737:chemistry* / Q000473:pathology* / Q000648:ultrastructure; D011708:Pylorus / Q000473:pathology* / Q000648:ultrastructure; D012794:Sialic Acids / Q000032:analysis
chemical_list: D014408:Biomarkers, Tumor; D005752:Gastric Mucins; D012794:Sialic Acids