Morphological features of the myenteric plexus of the stomach of the axolotl, Ambystoma mexicanum, revealed by immunocytochemistry.
The general morphology of the intramural innervation of the myenteric plexus of the axolotl stomach has been investigated using antisera raised against neuron-specific enolase and a microtubule-associated protein. Additionally, the occurrence of serotonin and several peptidergic neurotransmitter/neuromodulator substances was studied. Immunoreactivity for galanin, vasoactive intestinal polypeptide, substance P and neuromedin U was found in both fibres and intrinsic perikarya, whereas the serotonin and calcitonin gene-related peptide-like-substance-containing nerve fibres seemed to be of extrinsic origin. The axolotl stomach myenteric plexus appeared to be devoid of enkephalin-, neuropeptide Y-, somatostatin- and bombesin-like immunoreactive nerve fibres and nerve cell bodies. Double labelling experiments revealed the presence of a subpopulation of substance P/calcitonin gene-related peptide-like immunoreactive nerve fibres. Contrary to mammals, no coexistence of neuromedin U and substance P was found. Our findings illustrate that besides a number of similarities, considerable species differences exist between urodeles and anurans with regard to the organization of the enteric nervous system.
Gabriel
R
R
Department of Zoology, Jozsef Attila University, Széged, Hungary.
Timmermans
J P
JP
Adriaensen
D
D
De Groodt-Lasseel
M H
MH
Scheuermann
D W
DW
D000557:Ambystoma; D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000737:chemistry; D019004:Galanin; D007124:Immunoenzyme Techniques; D008869:Microtubule-Associated Proteins / Q000032:analysis; D009197:Myenteric Plexus / Q000737:chemistry / Q000166:cytology*; D009412:Nerve Fibers / Q000737:chemistry*; D009479:Neuropeptides / Q000032:analysis*; D010455:Peptides / Q000032:analysis; D010751:Phosphopyruvate Hydratase / Q000032:analysis; D012701:Serotonin / Q000032:analysis; D013270:Stomach / Q000737:chemistry / Q000294:innervation*; D013373:Substance P / Q000032:analysis; D014660:Vasoactive Intestinal Peptide / Q000032:analysis
D008869:Microtubule-Associated Proteins; D009479:Neuropeptides; D010455:Peptides; C056900:neuromedin U; D012701:Serotonin; D013373:Substance P; D014660:Vasoactive Intestinal Peptide; D019004:Galanin; D010751:Phosphopyruvate Hydratase; D015740:Calcitonin Gene-Related Peptide