Pitfalls in the double labelling immunoelectron microscopy using one face of the grid.
We compared two procedures in the double labelling immunoelectron microscopic method of hormone-storing cells: one-faced and two-faced procedures. With the two-faced procedure we got convincing results consistent with morphological finding, while we often got false results with the one-faced procedure. By the misleading one-faced procedure, the cell storing any one hormone will be misinterpreted as a multihormone storing cell. Therefore, it is concluded that the two-faced method is safer for the double labelling immunoelectron microscopic study.
Furuhata
S
S
Department of Pathology, Kitasato University School of Medicine, Kanagawa, Japan.
Nemoto
N
N
Miyazawa
S
S
Otani
M
M
Toya
S
S
Kameya
T
T
D000681:Amylases / Q000032:analysis / Q000276:immunology; D013006:Growth Hormone / Q000032:analysis / Q000276:immunology; D006801:Humans; D007106:Immune Sera / Q000032:analysis / Q000276:immunology; D007150:Immunohistochemistry; D016253:Microscopy, Immunoelectron / Q000295:instrumentation* / Q000379:methods*; D010903:Pituitary Gland, Anterior / Q000737:chemistry / Q000166:cytology / Q000648:ultrastructure; D011388:Prolactin / Q000032:analysis / Q000276:immunology
D007106:Immune Sera; D011388:Prolactin; D013006:Growth Hormone; D000681:Amylases