The human mast cell receptor binding site maps to the third constant domain of immunoglobulin E.
The characterization of the site on the IgE molecule which accommodates the high affinity receptor for IgE (Fc epsilon RI) should allow the design of IgE analogues which can be utilized to block allergic responses. Using chimeric human IgE molecules in which different constant region domains were exchanged with their murine homologues, we demonstrate here that the C epsilon 3 in its native configuration is essential for the binding to the alpha subunit of the human Fc epsilon RI. Deletion of the human C epsilon 2 from such chimeric molecules did not impair their ability to interact with the Fc epsilon RI, indicating that C epsilon 2 is not directly involved in the human Fc epsilon RI binding site and that C epsilon 3 alone is necessary and sufficient to account for most of the human Fc epsilon RI-binding capacity.
Nissim
A
A
Department of Chemical Immunology, Weizmann Institute of Science, Rehovot, Israel.
Eshhar
Z
Z
D000818:Animals; D000944:Antigens, Differentiation, B-Lymphocyte / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D001667:Binding, Competitive; D002678:Chimera; D002872:Chromosome Deletion; D006801:Humans; D015151:Immunoblotting; D007127:Immunoglobulin Constant Regions / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D007073:Immunoglobulin E / Q000235:genetics / Q000378:metabolism*; D007700:Kinetics; D008407:Mast Cells / Q000737:chemistry* / Q000276:immunology; D051379:Mice; D011485:Protein Binding; D011961:Receptors, Fc / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D017455:Receptors, IgE; D013816:Thermodynamics
D000944:Antigens, Differentiation, B-Lymphocyte; D007127:Immunoglobulin Constant Regions; D011961:Receptors, Fc; D017455:Receptors, IgE; D007073:Immunoglobulin E