title: Phylogenetic relationships of three porcine mycoplasmas, Mycoplasma hyopneumoniae, Mycoplasma flocculare, and Mycoplasma hyorhinis, and complete 16S rRNA sequence of M. flocculare.
abstract: The nucleotide sequence of the 16S rRNA gene of Mycoplasma flocculare was determined and was compared with the sequence of a related porcine mycoplasma, Mycoplasma hyopneumoniae. While the overall level of DNA-DNA homology was approximately 11%, sequence alignment of the two 16S rRNA genes yielded a homology value of more than 95%, emphasizing the highly conserved nature of the 16S rRNA gene. Multiple sequence alignments with other mollicutes indicated that M. flocculare, M. hyopneumoniae, and Mycoplasma hyorhinis form a subcluster within the fermentans phylogroup, and this subcluster is distinct from the Mycoplasma pneumoniae phylogroup. Thus, the three mycoplasmas isolated from porcine respiratory systems exhibit phylogenetic similarities.
authors.affiliation: Department of Microbiology, University of Alberta, Edmonton, Canada.
authors.forename: G W
authors.initials: GW
authors.lastname: Stemke
authors.forename: F
authors.initials: F
authors.lastname: Laigret
authors.forename: O
authors.initials: O
authors.lastname: Grau
authors.forename: J M
authors.initials: JM
authors.lastname: Bové
mesh_terms: D000818:Animals; D001483:Base Sequence; D004269:DNA, Bacterial; D008969:Molecular Sequence Data; D009174:Mycoplasma / Q000145:classification* / Q000235:genetics; D010802:Phylogeny; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D013552:Swine / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S