Luteinizing hormone requirements for ovulation in the rat.
Luteinizing hormone requirements for ovulation induction were studied in proestrous rats through detailed observation of the preovulatory surge, through various forms of LH injection under sodium pentobarbital blockade, and through estimation of LH uptake by the ovary. Blood LH levels in individual proestrous rats were obtained every 30 min and grouped according to their peak time (designated 0 h); mean LH levels higher than 7 and 5 ng/ml continued for 30 min and 2.5 h, respectively, the pituitary LH contents at 1400 and 2000 h on the day of proestrus were 2.1 and 0.7 micrograms, respectively, indicating that the amount of LH secreted during the surge was at least 1.4 micrograms. Single intravenous injections of 2 micrograms and 1 micrograms of pure rat LH (NIDDK-rLH-I-7; FSH and prolactin contaminations: 0.02% and less than 0.01%, respectively) to sodium pentobarbital-blocked rats induced ovulation in 4 out of 4 rats and 4 out of 6 rats, respectively, while 500 ng failed to induce ovulation in any (out of 7) rats. Two injections of 300 ng each with an interval of 20 min induced ovulation in 3 out of 8 rats, but if the interval was prolonged to between 30 and 120 min, 100% ovulation was obtained. Blood LH levels in these experiments indicated that a lower long-lasting LH level (about 5 ng/ml blood) is more important than a short, high level for ovulation induction. It was also shown that this level of LH could be given in separate doses if the interval was 30-120 min.(ABSTRACT TRUNCATED AT 250 WORDS)
Ishikawa
J
J
Central Research Laboratory, National Defense Medical College, Saitama, Japan.
D000818:Animals; D004305:Dose-Response Relationship, Drug; D005260:Female; D007986:Luteinizing Hormone / Q000008:administration & dosage / Q000494:pharmacology / Q000502:physiology*; D010053:Ovary / Q000378:metabolism; D010060:Ovulation / Q000187:drug effects / Q000502:physiology*; D010062:Ovulation Induction; D010424:Pentobarbital / Q000494:pharmacology; D011359:Proestrus / Q000502:physiology; D011388:Prolactin / Q000097:blood; D051381:Rats; D017208:Rats, Wistar
D011388:Prolactin; D007986:Luteinizing Hormone; D010424:Pentobarbital