[The expression of oncogenes in the development of human brain].
Seven oncogenes (c-myc, L-myc, N-myc, v-erbB, c-fos, Ha-ras and mos) were used as the probe to detect the total RNA of every part (cerebellum, temporal folium, frontal folium and occipital folium) in human brain from 2 cases of 6 mon fetus and the total RNA in fetal development (4 mon, 5 mon, 6 mon, 7 mon, and newborn) of human brain tissue by RNA dot hybridization analysis. The results showed that most oncogenes were expressed at high level in cerebellum and occipital folium of 6 mon fetus and at low level in temporal folium; there was similar expression of c-myc, L-myc and N-myc in fetal development of human brain tissue, and the highest level was in the 5 mon fetal brain. There was no expression of erbB gene in normal human brain. The high level expression of Ha-ras and c-fos genes was in the middle and later stages of fetal development. These results suggested that c-myc, L-myc, N-myc, erbB, Ha-ras, c-fos and mos genes might play an important role in the fetal development of human brain.
Sun
N
N
Laboratory of Recombinate DNA, Institute of Biochemistry and Molecular Biology.
Sun
Z
Z
D000818:Animals; D015152:Blotting, Northern; D001921:Brain / Q000196:embryology*; D015870:Gene Expression; D016762:Genes, fos; D016259:Genes, myc; D011905:Genes, ras; D006801:Humans; D011519:Proto-Oncogenes