title: Fluorometric enzyme immunoassay of basic fibroblast growth factor with monoclonal antibodies.
abstract: We compared three different strategies for measuring basic fibroblast growth factor (bFGF) by fluorometric enzyme immunoassay (EIA). After optimizing conditions, we found that a primary anti-bFGF MAb directly conjugated with peroxidase gave the best detection limit for recombinant bFGF (approximately 30 ng/L, 3 pg/assay tube) in a two-site sandwich assay. The detection limit of methods based on biotinylated primary MAbs or on secondary antibodies followed by streptavidin-conjugated peroxidase was slightly lower than that of the above method. Using the most sensitive EIA examined in this study, we made a preliminary measurement of immunoreactive bFGF in sera of apparently healthy people and found it to be 190 (SD 32) ng/L (n = 48), in agreement with an earlier reported value (30-206 ng/L). Also, the concentration of immunoreactive bFGF in sera was above normal in 19 of 31 patients with stomach cancer.
authors.affiliation: Gifu Pharmaceutical University, Department of Molecular Biology, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Kurobe
authors.forename: A
authors.initials: A
authors.lastname: Kato
authors.forename: Y
authors.initials: Y
authors.lastname: Takei
authors.forename: K
authors.initials: K
authors.lastname: Hayashi
mesh_terms: D000911:Antibodies, Monoclonal; D016222:Fibroblast Growth Factor 2 / Q000097:blood*; D006801:Humans; D007124:Immunoenzyme Techniques; D012016:Reference Values; D013274:Stomach Neoplasms / Q000097:blood
chemical_list: D000911:Antibodies, Monoclonal; D016222:Fibroblast Growth Factor 2