Human platelets deficient in dense granules contain normal amounts of pp60c-src.
We have determined that pp60c-src, a protein tyrosine kinase abundant in normal platelets, is present at comparable levels in platelets that are deficient in dense granules (Hermansky-Pudlak syndrome). Relative quantitation of pp60c-src was performed by immunoblot analysis after protein separation by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Our data suggest that human platelet dense granules, unlike chromaffin cell secretory granules, are not the major intracellular site of localization of pp60c-src.
Sorisky
A
A
Department of Biochemistry, University of Vermont College of Medicine, Burlington 05405.
Lages
B
B
Weiss
H J
HJ
Rittenhouse
S E
SE
D016115:Albinism, Oculocutaneous / Q000097:blood; D001792:Blood Platelets / Q000378:metabolism*; D006801:Humans; D015151:Immunoblotting; D010981:Platelet Storage Pool Deficiency / Q000097:blood*; D016392:Proto-Oncogene Proteins pp60(c-src) / Q000097:blood*
D016392:Proto-Oncogene Proteins pp60(c-src)