The extracellular matrix of rat spinal cord: a comparative study on the localization of hyaluronic acid, glial hyaluronate-binding protein, and chondroitin sulfate proteoglycan.
The localization of hyaluronic acid (HA), glial hyaluronate-binding protein (GHAP), and chondroitin sulfate (CS) proteoglycan was compared in cryostat sections of rat spinal cord. HA, GHAP, and CS proteoglycan were similarly distributed in white matter where they surrounded myelinated axons. In gray matter, large motoneurons were surrounded by a rim of reaction product in sections stained for HA and CS proteoglycan. GHAP immunoreactivity as well as HA had disappeared in hyaluronidase-digested sections, while CS proteoglycan immunoreactivity was not abolished under these conditions.
Bignami
A
A
Department of Pathology, Harvard Medical School, Boston, Massachusetts 02155.
Asher
R
R
Perides
G
G
D000818:Animals; D002352:Carrier Proteins / Q000032:analysis*; D011508:Chondroitin Sulfate Proteoglycans / Q000032:analysis*; D005109:Extracellular Matrix / Q000648:ultrastructure*; D018960:Hyaluronan Receptors; D006820:Hyaluronic Acid / Q000032:analysis*; D007106:Immune Sera; D007150:Immunohistochemistry; D009419:Nerve Tissue Proteins / Q000032:analysis*; D051381:Rats; D013116:Spinal Cord / Q000166:cytology*; D053675:Versicans
D002352:Carrier Proteins; D011508:Chondroitin Sulfate Proteoglycans; D018960:Hyaluronan Receptors; D007106:Immune Sera; D009419:Nerve Tissue Proteins; C508324:Vcan protein, rat; D053675:Versicans; D006820:Hyaluronic Acid