[An effective concentration method for human immunodeficiency virus type 1 (HIV-1)].
We designed an effective virus concentration method to prevent the infection with human immunodeficiency virus type 1 (HIV-1) in laboratories. The absorbent of Minicon concentrators (Amicon Division, M.R. Grace & Co.-Conn.) was changed to chitin, a mucopolysaccharide extracted from the shells of Japanese pink crab. HIV-1 in the supernatant of HIV-1 infected Molt-4 cells was concentrated by Minicon and the new concentrators. The new concentrator showed good concentration rate and equality of concentration speed.
Takasaki
T
T
Department of Bacteriology, Kinki University School of Medicine.
Sano
K
K
Tanaka
E
E
Morimatsu
S
S
Kifune
K
K
Tsurutani
R
R
Nakai
M
M
Yamaguchi
J
J
D002686:Chitin / Q000737:chemistry; D015497:HIV-1 / Q000302:isolation & purification*; D008828:Microbiological Techniques
D002686:Chitin