title: New fluorescent derivatives of cyclosporin for use in immunoassays.
abstract: The synthesis of new fluorescent derivatives of cyclosporin is described and their affinity with the specific Sandoz monoclonal antibody investigated. Synthesis was carried out using cyclosporin-C-hemisuccinate as the starting material with monodansylcadaverine, 4-bromomethyl-7-methoxycoumarin, and 4-bromomethyl-6,7-dimethoxycoumarin as labels. After extraction the derivatives were purified by HPLC and their binding affinity with the monoclonal antibody evaluated by the Incstar Cyclo-Trac SP radioimmunoassay. All three derivatives showed good binding and it is suggested that they may be of use in an immunoassay for measuring cyclosporin.
authors.affiliation: Department of Chemistry, Loughborough University, Leicestershire, UK.
authors.forename: M T
authors.initials: MT
authors.lastname: French
authors.forename: J N
authors.initials: JN
authors.lastname: Miller
authors.forename: N J
authors.initials: NJ
authors.lastname: Seare
authors.forename: D R
authors.initials: DR
authors.lastname: Lachno
authors.forename: M H
authors.initials: MH
authors.lastname: Yacoub
mesh_terms: D002851:Chromatography, High Pressure Liquid; D003524:Cyclosporins / Q000737:chemistry*; D005453:Fluorescence; D005455:Fluorescent Antibody Technique
chemical_list: D003524:Cyclosporins