title: Immunoelectron microscopic localization of microsomal alanine aminopeptidase.
abstract: The localization of microsomal alanine aminopeptidase was investigated in the rat kidney. Resin embedding failed to demonstrate the localization of the enzyme by immunogold labelling. Using a cryo-ultramicrotomy method the enzyme could be detected on the luminal side of the brush border membrane of proximal tubular cells and to a lesser degree in their mitochondria. Furthermore, vesicular structures labelled with gold were found in the cytoplasma in the apical region of these cells.
authors.affiliation: Institute of Biochemistry, Faculty of Medicine, University of Halle, Germany.
authors.forename: U
authors.initials: U
authors.lastname: Kettmann
authors.forename: B
authors.initials: B
authors.lastname: Humbel
authors.forename: H J
authors.initials: HJ
authors.lastname: Holzhausen
authors.forename: H
authors.initials: H
authors.lastname: Bahn
authors.forename: H
authors.initials: H
authors.lastname: Aurich
mesh_terms: D000626:Aminopeptidases / Q000032:analysis*; D000818:Animals; D018826:CD13 Antigens; D007150:Immunohistochemistry; D007668:Kidney / Q000201:enzymology*; D007687:Kidney Tubules, Proximal / Q000201:enzymology* / Q000648:ultrastructure; D016253:Microscopy, Immunoelectron; D008861:Microsomes / Q000201:enzymology; D008871:Microvilli / Q000201:enzymology; D008928:Mitochondria / Q000201:enzymology; D051381:Rats; D013194:Staining and Labeling
chemical_list: D000626:Aminopeptidases; D018826:CD13 Antigens