Novel method for performing carbonic anhydrase histochemistry and immunocytochemistry on cryosections.
It is difficult to correlate structure with function in the kidney because of the extensive cell heterogeneity. Carbonic anhydrase (CA) is an enzyme that mediates renal acidification and is found predominantly in proximal tubule and collecting duct cells. We modified Hansson's method for histochemically identifying cellular CA activity on PLP-fixed rabbit kidney sections mounted on Millipore filters, and then removed the filters to perform peanut lectin and antibody labeling on the same sections. There was adequate preservation of morphology, and individual cells could be identified with CA activity in the cytosol and specific antibody or lectin labeling on the cell surfaces.
Matsumoto
T
T
Department of Pediatrics, Albert Einstein College of Medicine, Bronx, New York 10461.
Schwartz
G J
GJ
D000818:Animals; D000911:Antibodies, Monoclonal; D002256:Carbonic Anhydrases / Q000032:analysis*; D003600:Cytosol / Q000201:enzymology; D005629:Frozen Sections; D006651:Histocytochemistry; D007150:Immunohistochemistry; D007685:Kidney Tubules, Collecting / Q000737:chemistry / Q000166:cytology* / Q000201:enzymology; D007687:Kidney Tubules, Proximal / Q000737:chemistry / Q000166:cytology* / Q000201:enzymology; D037102:Lectins; D019887:Peanut Agglutinin; D011817:Rabbits; D013194:Staining and Labeling
D000911:Antibodies, Monoclonal; D037102:Lectins; D019887:Peanut Agglutinin; D002256:Carbonic Anhydrases