Sister-chromatid exchange in highly purified human CD4+ and CD8+ lymphocytes.
Sister-chromatid exchange (SCE) frequencies were determined in human peripheral blood CD4+ and CD8+ T lymphocyte subpopulations which were rapidly and highly purified from pooled T lymphocytes by immunological methods. The purified lymphocytes were stimulated with phytohemagglutinin (PHA) for 4 days. CD4+ lymphocytes showed significantly higher SCE frequencies than autologous CD8+ lymphocytes when measured simultaneously after identical bromodeoxyuridine (BrdU) incubation times. Differences in SCE frequencies between CD4+ and CD8+ lymphocytes were also detected when mitomycin C (MMC) was added to the cultures. Higher SCE frequencies in CD4+ lymphocytes were associated with lower proliferating rate indices (PRI) as compared to autologous CD8+ lymphocytes. Abnormalities in CD4+ T lymphocyte function and number in peripheral blood have been observed in several diseases characterized by immunological disorders. Thus, our data may suggest a link between some immunological disturbances and abnormal SCE frequencies in T lymphocyte subsets.
Sarri
C
C
Genetics Division, Agia Sofia Children's Hospital, Athens, Greece.
Baxevanis
C N
CN
Côté
G B
GB
Reclos
G J
GJ
Sarafidou
E
E
Spanos
T
T
Papanastasiou
M
M
Grigoriadou
M
M
Papamichail
M
M
D001973:Bromodeoxyuridine; D015704:CD4 Antigens / Q000235:genetics; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology* / Q000276:immunology; D016827:CD8 Antigens / Q000235:genetics; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D006801:Humans; D007125:Immunogenetics; D008213:Lymphocyte Activation; D016685:Mitomycin / Q000494:pharmacology; D010835:Phytohemagglutinins; D012854:Sister Chromatid Exchange; D016176:T-Lymphocyte Subsets / Q000166:cytology* / Q000276:immunology; D013601:T-Lymphocytes / Q000166:cytology* / Q000276:immunology
D015704:CD4 Antigens; D016827:CD8 Antigens; D010835:Phytohemagglutinins; D016685:Mitomycin; D001973:Bromodeoxyuridine