title: Isolation of a yeast artificial chromosome clone that spans the (12;16) translocation breakpoint characteristic of myxoid liposarcoma.
abstract: Cytogenetic analysis of liposarcomas has demonstrated that translocation (12;16) (q13.3;p11.2) is characteristic of the myxoid subtype of this adipose tissue tumor. Our previous results suggested that the GLI gene is close to the translocation breakpoint on chromosome 12. We now describe a yeast artificial chromosome (YAC) that contains GLI and spans the chromosome 12 region involved in the t(12;16) breakpoint. This clone will permit rapid definition of the genetic region surrounding the breakpoint and allow isolation of the gene presumably affected by the translocation.
authors.affiliation: Eleanor Roosevelt Institute, Denver, Colorado 80206.
authors.forename: R M
authors.initials: RM
authors.lastname: Gemmill
authors.forename: M J
authors.initials: MJ
authors.lastname: Mendez
authors.forename: C M
authors.initials: CM
authors.lastname: Dougherty
authors.forename: S
authors.initials: S
authors.lastname: Paulien
authors.forename: M
authors.initials: M
authors.lastname: Liao
authors.forename: D
authors.initials: D
authors.lastname: Mitchell
authors.forename: S A
authors.initials: SA
authors.lastname: Jankowski
authors.forename: J M
authors.initials: JM
authors.lastname: Trent
authors.forename: C
authors.initials: C
authors.lastname: Berger
authors.forename: A A
authors.initials: AA
authors.lastname: Sandberg
mesh_terms: D015825:Chromosomes, Fungal; D002881:Chromosomes, Human, Pair 12; D002885:Chromosomes, Human, Pair 16; D003001:Cloning, Molecular; D015723:Gene Library; D015894:Genome, Human; D006801:Humans; D008080:Liposarcoma / Q000235:genetics*; D014178:Translocation, Genetic