Heat-shock gene expression excludes hepatic acute-phase gene expression after resuscitation from hemorrhagic shock.
BACKGROUND
To determine whether the gene expression of both acute-phase reactants (APR) and the major heat-shock protein (hsp-72) can occur simultaneously, transcriptional rates were measured during shock and resuscitation.


METHODS
A nuclear runoff technique was applied to hepatic biopsy specimens obtained from pigs before shock, during 40% blood volume hemorrhagic shock (1 and 2 hours), and after resuscitation (4 and 6 hours).


RESULTS
Shock-induced transcription of hsp-72 was elevated elevenfold over sham operation at 2 hours (p less than 0.02, Mann-Whitney rank test). Individually shocked animals did not transcribe both classes of stress genes but segregated into two groups: (1) strong APR transcriptional responders and (2) hsp-72 transcriptional responders. In group 2, APR transcription was significantly suppressed. Antichymotrypsin transcription was an average of eighteenfold lower in group 2 versus group 1 (p less than 0.05 at 1,2, and 6 hours).


CONCLUSIONS
Different classes of stress protein genes are not transcribed simultaneously. We infer that their increased accumulation at the mRNA level is the result of sequential transcription. Hsp-72 transcription excludes that of the APR genes that may be critical to survival after stress.
Schoeniger
L O
LO
Department of Surgery, Johns Hopkins University School of Medicine, Baltimore, MD 21205.
Reilly
P M
PM
Bulkley
G B
GB
Buchman
T G
TG
D000209:Acute-Phase Proteins / Q000235:genetics*; D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D006360:Heat-Shock Proteins / Q000235:genetics*; D006439:Hemodynamics; D008099:Liver / Q000378:metabolism / Q000502:physiology*; D012313:RNA / Q000378:metabolism; D012151:Resuscitation; D012771:Shock, Hemorrhagic / Q000235:genetics* / Q000503:physiopathology / Q000628:therapy; D013552:Swine; D014158:Transcription, Genetic
D000209:Acute-Phase Proteins; D006360:Heat-Shock Proteins; D012313:RNA