title: Expression of the genes for alpha inhibin, beta A inhibin and follistatin in the ovaries of Booroola ewes which were homozygotes or non-carriers of the fecundity gene FecB.
abstract: Ovine cDNA probes for the alpha and beta A inhibin subunits and for follistatin were used to investigate the mRNA species for these hormones in ovaries obtained during the luteal phase of the oestrous cycle, from Booroola ewes which were homozygous carriers (BB) or non-carriers (++) of the FecB gene. BB ewes had significantly higher concentrations of peripheral FSH and LH immunoreactivity than ++ ewes, but the peripheral inhibin immunoreactivity and ovarian inhibin and progesterone secretion rates were not significantly different between genotypes. No gene-specific differences in the number or size of mRNA transcripts detected by Northern blotting were noted for any of these genes. A single alpha inhibin mRNA species at 1.5 kb was observed in the follicle RNA from ++ and BB ovaries. Low amounts of alpha inhibin hybridization were discerned occasionally in ++ and BB stroma and also in BB, but not in ++, corpora lutea. The beta A inhibin gene was expressed only in the follicles from both ++ and BB ovaries. At least three beta A inhibin transcripts were observed; one at 7.5 kb and at least two between 1.4 and 5.0 kb. The follistatin cDNA probe detected two major transcripts at 2.7 and 1.5 kb and a minor band at 0.5 kb in both follicle and corpora lutea RNA. Densitometry of the Northern blots revealed no significant gene-specific differences in the levels of alpha inhibin and follistatin gene mRNA transcripts.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Gene Expression Laboratory, Wallaceville Animal Research Centre, Upper Hutt, New Zealand.
authors.forename: J S
authors.initials: JS
authors.lastname: Fleming
authors.forename: D J
authors.initials: DJ
authors.lastname: Tisdall
authors.forename: P J
authors.initials: PJ
authors.lastname: Greenwood
authors.forename: N L
authors.initials: NL
authors.lastname: Hudson
authors.forename: D A
authors.initials: DA
authors.lastname: Heath
authors.forename: K P
authors.initials: KP
authors.lastname: McNatty
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D005260:Female; D005298:Fertility / Q000235:genetics*; D005640:Follicle Stimulating Hormone / Q000097:blood; D038681:Follistatin; D005786:Gene Expression Regulation; D006023:Glycoproteins / Q000235:genetics* / Q000378:metabolism; D006720:Homozygote; D007265:Inhibins / Q000097:blood / Q000235:genetics*; D007986:Luteinizing Hormone / Q000097:blood; D010053:Ovary / Q000378:metabolism*; D010060:Ovulation / Q000235:genetics; D011374:Progesterone / Q000097:blood; D012313:RNA / Q000235:genetics; D012756:Sheep
chemical_list: D038681:Follistatin; D006023:Glycoproteins; D011374:Progesterone; D007265:Inhibins; D012313:RNA; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone