The efficacy of cyclosporin A, FK-506 and prednisolone to modify the adoptive transfer of experimental allergic encephalomyelitis (EAE).
The in vitro potency of the immunosuppressants Cyclosporin A (CsA), FK-506 and Prednisolone was assessed using the adoptive transfer model of EAE in the Lewis rat. Co-culture of encephalitogen-sensitised splenic leukocytes with Prednisolone did not inhibit the transfer of disease to naive histocompatible recipients despite significant suppression of neuroantigen-stimulated leukocyte proliferation by the drug. The addition of CsA (100 nM) to cultures inhibited the induction of adoptive EAE but a lower dose of the agent (10 nM) did not prevent the development of clinico-histopathological signs of disease. FK-506 (1 nM) was 100 times more effective than CsA at suppressing adoptive EAE thus emphasising the usefulness of the model in determining the relative efficacy of compounds to modify cell-dependent autoimmune disease.
Bolton
C
C
Pharmacology Group, School of Pharmacy & Pharmacology, University of Bath, Claverton Down.
D000818:Animals; D001835:Body Weight; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D016572:Cyclosporine / Q000627:therapeutic use*; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology* / Q000517:prevention & control; D007116:Immunization, Passive; D017708:Leukocyte Transfusion; D007962:Leukocytes / Q000276:immunology; D008297:Male; D004676:Myelin Basic Protein / Q000276:immunology / Q000494:pharmacology; D011239:Prednisolone / Q000627:therapeutic use*; D051381:Rats; D011917:Rats, Inbred Lew; D013154:Spleen / Q000166:cytology / Q000276:immunology; D016559:Tacrolimus / Q000627:therapeutic use*
D004676:Myelin Basic Protein; D016572:Cyclosporine; D011239:Prednisolone; D016559:Tacrolimus