Acute and subacute toxicity of 7.5% hypertonic saline/6% dextran-70 (HSD) in dogs. 1. Serum immunoglobulin and complement responses.
Clinical use of modern dextran solutions has been limited by concerns of anaphylactoid reactions. To assess the short-term antigenic response to 7.5% hypertonic saline in 6% Dextran-70 (HSD), sera were obtained from dogs involved in the acute and subacute toxicology testing of HSD and its individual components, and analyzed for IgG, IgM and C3 complement. In separate studies, beagles were infused i.v. with a single dose of HSD or its components at 20 ml kg-1 (the maximum tolerated dose; MTD), or the MTD daily for 14 days, and serum was obtained prior to and at various times after infusion up to 14 days. In both studies, despite serum dextran concentrations exceeding 2000 mg dl-1, no induction of IgG, IgM or C3 complement concentrations were observed. In addition, serum IgG immunoelectrophoretic patterns were of normal curvature, position and intensity; the immunoprecipitin bands were not displaced, bowed, inhibited or thicker than the normal preinfusion immunoelectrophoretograms. The data suggest that single or multiple HSD i.v. injections, as much as five times the proposed therapeutic level for the treatment of hypovolemia, evoked no increase in antibody titers in dogs. Therefore, therapeutic use of HSD in the treatment of hemorrhagic shock should not be associated with widespread concomitant allergic complications.
Summary
J J
JJ
Division of Military Trauma Research, Letterman Army Institute of Research, Presidio of San Francisco, CA 94129-6800.
Dubick
M A
MA
Zaucha
G M
GM
Kilani
A F
AF
Korte
D W
DW
Wade
C E
CE
D000818:Animals; D002850:Chromatography, Gel; D003165:Complement System Proteins / Q000378:metabolism*; D003911:Dextrans / Q000008:administration & dosage / Q000633:toxicity*; D004285:Dogs; D004338:Drug Combinations; D005260:Female; D007074:Immunoglobulin G / Q000378:metabolism*; D007075:Immunoglobulin M / Q000378:metabolism; D008297:Male; D012462:Saline Solution, Hypertonic / Q000008:administration & dosage / Q000633:toxicity*
D003911:Dextrans; D004338:Drug Combinations; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D012462:Saline Solution, Hypertonic; D003165:Complement System Proteins