Detection of VAC-beta (annexin-8) in human placenta.
A 36 kDa calcium/phospholipid binding protein in human placenta was identified as VAC-beta (annexin-8) by a combination of immunological and peptide mapping analyses. The protein is a minor product in placenta, accounting for less than 1% of extracted annexins. From 150 g of tissue, only 100 micrograms of the protein was isolated. By anion-exchange chromatography on diethylaminoethyl-cellulose annexin-8 coeluted with annexin-3. By gel filtration, the protein chromatographed as a broad peak, where half the product eluted as a monomer and half eluted as a heterodimer that was associated with a 10 kDa subunit. The combination of annexin-8 being a minor component in standard annexin preparations and it co-eluting with annexin-3 by ion exchange chromatography are likely to account for the failure of other labs to characterize the product.
Pepinsky
R B
RB
Department of Protein Chemistry, Biogen, Inc., Cambridge, MA 02142.
Hauptmann
R
R
D000595:Amino Acid Sequence; D000818:Animals; D017302:Annexins; D015153:Blotting, Western; D002848:Chromatography, DEAE-Cellulose; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D008969:Molecular Sequence Data; D010449:Peptide Mapping; D010455:Peptides / Q000032:analysis*; D010920:Placenta / Q000737:chemistry*; D011247:Pregnancy; D011817:Rabbits; D016415:Sequence Alignment
D017302:Annexins; D010455:Peptides