Rapid differentiation of Pseudomonas pseudomallei from Pseudomonas cepacia.
The Minitek disc system was utilized for the differentiation of Pseudomonas pseudomallei, the causative agent of melioidosis, from Ps. cepacia. The system was simple to use, inexpensive, and furnished rapid, clear-cut test results after 4 h. This procedure is suitable for differentiating soil bacteria presumptively identified as Ps. pseudomallei, Ps. cepacia or flavobacteria, and for the rapid confirmation of the presumptive identification of either Ps. pseudomallei or Ps. cepacia obtained by commercial identification-kit systems in the clinical laboratory.
Ashdown
L R
LR
Anton Breinl Centre for Tropical Health and Medicine, James Cook University of North Queensland, Townsville, Australia.
D016956:Burkholderia cepacia / Q000201:enzymology / Q000302:isolation & purification*; D016957:Burkholderia pseudomallei / Q000201:enzymology / Q000302:isolation & purification*; D002262:Carboxy-Lyases / Q000378:metabolism; D003470:Culture Media; D006867:Hydrolases / Q000378:metabolism; D009566:Nitrates / Q000378:metabolism; D009598:Nitrophenylgalactosides / Q000378:metabolism; D012988:Soil Microbiology
D003470:Culture Media; D009566:Nitrates; C055012:2-nitrophenylgalactoside; D009598:Nitrophenylgalactosides; D006867:Hydrolases; C019723:arginine deiminase; D002262:Carboxy-Lyases; C021520:lysine decarboxylase