Inhibition of RNA- and DNA-synthesis by citrinin and its effects on DNA precursor-metabolism in V79-E cells.
1. The RNA synthesis of V79-E cells was inhibited by the mycotoxin citrinin time- and concentration-dependently. 2. Among the different RNA species mainly the rRNA synthesis was found to be inhibited by 200 microM citrinin. 3. At different precursor concentrations DNA synthesis was inhibited by citrinin after 30 min at least whereas labelling of the acid soluble fractions was found to be 3-fold higher than in untreated cells. 4. Remarkable perturbation of the DNA precursor metabolism, including release of precursor into the medium, was found to occur during citrinin treatment.
Wasternack
C
C
Institute of Plant Biochemistry, Halle/Saale, Germany.
Weisser
J
J
D000818:Animals; D002460:Cell Line; D002953:Citrinin / Q000737:chemistry / Q000494:pharmacology*; D006224:Cricetinae; D004247:DNA / Q000096:biosynthesis* / Q000187:drug effects / Q000378:metabolism; D007700:Kinetics; D015394:Molecular Structure; D012313:RNA / Q000096:biosynthesis* / Q000187:drug effects
D002953:Citrinin; D012313:RNA; D004247:DNA