Effect of phospholipase A2 and ethanol on the survival of acinar cells isolated from the rat pancreas.
The study was undertaken to investigate the effect of phospholipase A2 (PLA2) on the viability of isolated pancreatic acinar cells (PACs) and its possible synergy with ethanol. The survival of PACs may be reduced by PLA2 in a dose-dependent manner. In the presence of 0.25 microgram PLA2.(10(6) cells)-1, all cells died within less than 4 h of incubation at 37 degrees C. A quantity of 180 mM ethanol, which alone was not lethal for PACs, increased the rate of cell death induced by PLA2 at a certain concentration of this enzyme. The present results imply that (1) PLA2 is able to damage PACs in the absence of additional substrates, and (2) at an appropriate activity of PLA2, ethanol may amplify the toxic effects of this lytic enzyme and force autodigestion of the pancreas.
Letko
G
G
Division of Experimental Surgery, Medical Academy of Magdeburg, FRG.
Siech
M
M
D000208:Acute Disease; D000818:Animals; D000431:Ethanol / Q000494:pharmacology*; D005260:Female; D066298:In Vitro Techniques; D010179:Pancreas / Q000166:cytology / Q000187:drug effects*; D010195:Pancreatitis / Q000209:etiology; D010741:Phospholipases A / Q000494:pharmacology*; D054467:Phospholipases A2; D051381:Rats; D013997:Time Factors
D000431:Ethanol; D010741:Phospholipases A; D054467:Phospholipases A2