[Protective effect of 7-oxo-prostacyclin on myocardial calcium overload in the isolated rat heart].
The protective effect of a stable derivative of prostacyclin (7-oxo PGI2) was studied on the model of calcium overload (Ca2+ paradox) 48 h after i.m. administration of the drug in the dose of 50 micrograms/kg. In the isolated rat heart perfused at 37 degrees C and a constant perfusion pressure of 75 Torr (Langendorff preparation) Ca2+ paradox was induced by 3 min perfusion with calcium-free Krebs-Henseleit solution and a subsequent 10 min perfusion with a normal calcium-containing solution. The late protective effect of 7-oxo PGI2 was manifested by improved recovery of heart function (increase of contractility by 50%) and by better preservation of the content of macroergic phosphates (70% sigma ADN) during the Ca2+ repletion phase and of myocardial ultrastructure (sarcolemma) already during the Ca2+ depletion phase. The protective effect of 7-oxo PGI2 can be accounted for by stimulation of Na, K-ATPase activity, otherwise decreased during calcium depletion phase, and by the consequent prevention of alterations in sodium and calcium homeostasis. (Tab. 1, Fig. 4, Ref. 41.)
Ravingerová
T
T
Ustav pre výskum srdca SAV, Bratislava, CSFR.
Tribulová
N
N
Dzurba
A
A
Ziegelhöffer
A
A
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D011464:Epoprostenol / Q000031:analogs & derivatives* / Q000494:pharmacology; D006321:Heart / Q000187:drug effects; D066298:In Vitro Techniques; D008297:Male; D009200:Myocardial Contraction / Q000187:drug effects; D009206:Myocardium / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
C033705:7-oxoprostaglandin I2; D011464:Epoprostenol; D002118:Calcium