Identification of the ferrioxamine B receptor, FoxB, in Escherichia coli K12.
The photoreactive p-azidobenzoyl analog of ferrioxamine B was used to show that ferrioxamine-B-mediated iron transport is separate and distinct from coprogen-mediated iron transport in Escherichia coli. Photolysis of this analog inhibited uptake of [59Fe]ferrioxamine B but not [59Fe]ferrichrome. Conversely, photolysis of the p-azidobenzoyl analog of coprogen B inhibited uptake of [59Fe]coprogen but not [59Fe]ferrioxamine B or [59Fe]ferrichrome. Photolabeling of outer membranes with p-azidobenzoyl-[59Fe]ferrioxamine B resulted in the labeling of two iron-regulated peptides with molecular masses of about 66 and 26 kDa. Expression of these peptides was increased when ferrioxamine B was the sole iron source. Both peptides were present in outer membrane preparations of the fhuF mutant H1717, but the 66 kDa peptide was not inducible. These results are evidence for an outer membrane receptor in E. coli unique for linear ferrioxamines.
Nelson
M
M
Department of Microbiology, University of Texas, Austin 78712.
Carrano
C J
CJ
Szaniszlo
P J
PJ
D001425:Bacterial Outer Membrane Proteins / Q000302:isolation & purification / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D003676:Deferoxamine / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D029968:Escherichia coli Proteins; D005290:Ferric Compounds / Q000378:metabolism*; D005291:Ferrichrome / Q000378:metabolism; D005838:Genotype; D006877:Hydroxamic Acids / Q000378:metabolism; D007501:Iron / Q000378:metabolism*; D007502:Iron Chelating Agents / Q000378:metabolism; D007700:Kinetics; D008970:Molecular Weight; D010782:Photolysis; D011956:Receptors, Cell Surface; D013329:Structure-Activity Relationship
D001425:Bacterial Outer Membrane Proteins; D029968:Escherichia coli Proteins; D005290:Ferric Compounds; C489523:FoxB protein, E coli; D006877:Hydroxamic Acids; D007502:Iron Chelating Agents; D011956:Receptors, Cell Surface; D005291:Ferrichrome; C002577:ferrioxamine B; C010896:coprogen; D007501:Iron; D003676:Deferoxamine