Somatic cell hybrid mapping of human chromosome band 5q31: a region important to hematopoiesis.
As a means of characterizing the distal long arm of chromosome 5, in particular, the region spanning 5q23-->q31, we analyzed somatic cell hybrids prepared from cells with overlapping chromosomal rearrangements. In one hybrid, the derivative chromosome 5 from a patient with acute myeloid leukemia (AML) de novo, whose bone marrow cells had a balanced translocation, t(5;7)(q31;q22), involving chromosome band 5q31, was isolated in a somatic cell hybrid (B294). In addition, we prepared somatic cell hybrids from a lymphoblastoid cell line (CC) derived from a patient who has a constitutional interstitial deletion of chromosome 5 spanning 5q23.1-->q31.1. By a combination of Southern hybridization analysis and fluorescent in situ hybridization, we constructed a map dividing 5q23-->q31 into four regions. We can assign genes to these regions and relate them to anonymous RFLP markers that have been genetically mapped.
Neuman
W L
WL
Department of Medicine, University of Chicago, IL 60637.
Le Beau
M M
MM
Farber
R A
RA
Lindgren
V
V
Westbrook
C A
CA
D000208:Acute Disease; D000328:Adult; D001853:Bone Marrow / Q000473:pathology; D002460:Cell Line; D002478:Cells, Cultured; D002871:Chromosome Banding; D002874:Chromosome Mapping; D002895:Chromosomes, Human, Pair 5; D015342:DNA Probes; D005260:Female; D006410:Hematopoiesis / Q000235:genetics*; D006801:Humans; D006822:Hybrid Cells; D007951:Leukemia, Myeloid / Q000235:genetics* / Q000473:pathology
D015342:DNA Probes