Co-expression of T-cell receptor beta and delta mRNA detected at high frequency in hybridomas derived from adult thymus.
Two different hybridoma collections from adult C3H/HeJ thymus were generated in order to analyse T-cell receptor (TcR) rearrangements, surface expression of T-cell receptors and differentiation markers as well as lymphokine production. Large, low density thymocytes were either directly fused to the thymoma BW 5147 alpha-beta- variant, or fused after stimulation with Concanavalin A in the presence of interleukin-2 for 48 h. The hybrids obtained from Concanavalin A-stimulated cells represented rather mature thymocytes, with regard to TcR rearrangements and surface T-cell receptor expression. The collection of hybrids derived from freshly isolated large thymocytes contained cells in various stages of T-cell development. An unexpectedly large number of hybrids (46 out of 84) from this group expressed full-length C beta together with full-length, or shorter, C delta mRNA. This finding suggests that a considerable proportion of alpha beta T cells proceeds through a stage in development where delta genes are being rearranged and transcribed.
Spetz
A L
AL
Institut Pasteur, Paris, France.
Casrouge
A
A
Cochet
M
M
Kourilsky
P
P
Larsson-Sciard
E L
EL
D000818:Animals; D015704:CD4 Antigens / Q000032:analysis; D005434:Flow Cytometry; D006825:Hybridomas / Q000737:chemistry*; D007376:Interleukin-2 / Q000096:biosynthesis; D008213:Lymphocyte Activation; D051379:Mice; D008809:Mice, Inbred C3H; D012333:RNA, Messenger / Q000032:analysis*; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000032:analysis / Q000235:genetics*; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000032:analysis / Q000235:genetics*; D013601:T-Lymphocytes / Q000502:physiology*; D013950:Thymus Gland / Q000276:immunology*
D015704:CD4 Antigens; D007376:Interleukin-2; D012333:RNA, Messenger; D016693:Receptors, Antigen, T-Cell, alpha-beta; D016692:Receptors, Antigen, T-Cell, gamma-delta