title: An estimate of the number of genes in the Huntington disease gene region and the identification of 13 transcripts in the 4p16.3 segment.
abstract: Physical mapping and genetic linkage studies have positioned the Huntington disease (HD) gene to a relatively large genomic region in the distal portion of the short arm of human chromosome 4 (4p16.3). To estimate the number of genes present in this region and to identify candidate disease genes, several clones that map to the 4p16.3 segment have been examined for clusters of CpG-rich restriction sites and transcribed sequences. Thirteen expressed sequences were identified and were shown by pulsed-field gel electrophoresis not to cluster into a small segment of the 4p16.3 band. The frequency of transcripts in these clones suggests that the putative HD gene region contains about 100 genes.
authors.affiliation: Department of Molecular Biology, Wayne State University, Detroit, Michigan 48201.
authors.forename: L
authors.initials: L
authors.lastname: Carlock
authors.forename: D
authors.initials: D
authors.lastname: Wisniewski
authors.forename: M
authors.initials: M
authors.lastname: Lorincz
authors.forename: A
authors.initials: A
authors.lastname: Pandrangi
authors.forename: T
authors.initials: T
authors.lastname: Vo
mesh_terms: D015152:Blotting, Northern; D015139:Blotting, Southern; D002478:Cells, Cultured; D002874:Chromosome Mapping; D002894:Chromosomes, Human, Pair 4; D004247:DNA / Q000378:metabolism; D016521:Electrophoresis, Gel, Pulsed-Field; D006801:Humans; D006816:Huntington Disease / Q000235:genetics*; D008745:Methylation; D015183:Restriction Mapping; D014158:Transcription, Genetic
chemical_list: D004247:DNA