Localization of the citrate synthase (CS) gene to the p12-p13 bands of chromosome 5 in pigs by in situ hybridization.
Citrate synthase (CS) is a key enzyme of the Krebs tricarboxylic acid cycle. A 1.4 kb porcine CS cDNA probe was used to chromosomally localize the CS gene in pigs by in situ hybridization. Two in situ hybridization experiments were conducted. Although the first experiment indicated a distinct signal on the 5p12-p13 bands, a secondary signal was observed on the 13q24-q32 bands. Hence, a second in situ hybridization experiment was conducted at higher stringency. The results demonstrated a consistent signal on the 5p12-p13 bands, and the signal on chromosome 13 was scattered with no prominent secondary peak. The CS gene was therefore assigned to the p12-p13 bands of chromosome 5 in pigs.
Chaudhary
R
R
Department of Animal Breeding and Genetics, Swedish University of Agricultural Sciences, Uppsala.
Chowdhary
B P
BP
Harbitz
I
I
Gustavsson
I
I
Evans
C T
CT
D000818:Animals; D002871:Chromosome Banding; D002874:Chromosome Mapping / Q000379:methods*; D002950:Citrate (si)-Synthase / Q000235:genetics*; D017403:In Situ Hybridization; D013552:Swine
D002950:Citrate (si)-Synthase