[Chemistry and immunochemistry analysis of the soluble antigens of newborn larvae of Trichinella spiralis].
The soluble antigens of newborn larvae of Trichinella spiralis were characterized in terms of molecular weight of protein, glycoprotein and lipoprotein contents, and immunochemistry. After the antigens were separated with SDS-PAGE and then followed by ultrasensitive silver staining, at least 40 bands of proteins were noted. Correspondingly, 28 bands of glycoproteins and 9 bands of lipoproteins were revealed as the similar gels were stained by the hypersensitive periodic acid silver and Nile's blue staining respectively. Six specific bands were recognized using polyclonal antibodies in the serum of rabbits immunized with antigens of newborn larvae by immunoblotting. All of them are glycoproteins. The components of the antigens with molecular weight (MW) 41.5 kd, 40 kd, 29.5 kd, 25 kd, 11 kd and 18 kd were found to be species specific and newborn larva stage-specific target antigen relevantly.
Zhang
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Feng
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D000818:Animals; D000947:Antigens, Helminth / Q000737:chemistry / Q000276:immunology*; D015153:Blotting, Western; D000939:Epitopes / Q000032:analysis*; D006023:Glycoproteins / Q000032:analysis; D007120:Immunochemistry; D007814:Larva; D011817:Rabbits; D013045:Species Specificity; D014234:Trichinella / Q000276:immunology*
D000947:Antigens, Helminth; D000939:Epitopes; D006023:Glycoproteins