Histamine release and morphological changes in basophilic granulocytes of atopic asthmatics induced by antigen, anti-IgE and Ca ionophore A 23187.
Morphological changes of basophils from atopic asthmatics were compared among antigen, anti-IgE and Ca ionophore A23187 stimulation. 1. Antigen induced rapid and marked increase of histamine release from basophils compared with Ca ionophore A23187. 2. The decrease in number of basophils following stimulation with the agents was significantly higher in antigen stimulation than in Ca ionophore A23187 stimulation. 3. The increased ratio of short to long axis diameter (L/Sb ratio) of the cells, which shows an increased motility of basophils, was significantly higher in antigen stimulation. The ratio did not change by stimulation with Ca ionophore A23187. 4. Stimulation of basophils by Ca ionophore A23187 induced marked increase in mean diameter (MD) of the cells. Activation of basophils by anti-IgE was similar to that by antigen, but slower in start and shorter in duration compared with antigen. The results show that an increase in motility is essential for release mechanism of chemical mediators from basophils in antigen and anti-IgE stimulation, but not in Ca ionophore A23187 stimulation.
Tanizaki
Y
Y
Division of Medicine, Misasa Medical Branch, Okayama University Medical School.
Nakagawa
S
S
Kimura
I
I
D000328:Adult; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D000941:Antigens / Q000276:immunology*; D000944:Antigens, Differentiation, B-Lymphocyte / Q000276:immunology; D001249:Asthma / Q000276:immunology / Q000473:pathology / Q000503:physiopathology*; D001491:Basophils / Q000276:immunology* / Q000473:pathology; D000001:Calcimycin / Q000276:immunology*; D002465:Cell Movement; D005260:Female; D006636:Histamine Release; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology*; D008297:Male; D011961:Receptors, Fc / Q000276:immunology; D017455:Receptors, IgE
D000888:Antibodies, Anti-Idiotypic; D000941:Antigens; D000944:Antigens, Differentiation, B-Lymphocyte; D011961:Receptors, Fc; D017455:Receptors, IgE; C026408:anti-IgE antibodies; D007073:Immunoglobulin E; D000001:Calcimycin