title: A cytologic, ultrastructural and immunocytochemical comparison of tumor cells and cell cultures originating in invasive bladder carcinoma.
abstract: Tumor cells and urine-voided cells from patients with invasive bladder carcinoma as well as from healthy patients were examined cytologically, ultrastructurally and immunocytochemically. The ultrastructure of tumor cells showed an abundant, dilated, rough endoplasmic reticulum in the form of membrane-bound vacuoles full of granular to fibrillar material located perinuclearly and/or paranuclearly. Some cells exhibited enlarged modified lysosomes containing sparce flocculent and particulate precipitate. Papanicolaou staining of these cells showed two basophilic cytoplasmic textures, one green glossy-patchy, perinuclearly and/or paranuclearly, well segregated from the other texture of peripheral hematoxylinophilic foamy cytoplasm, comparable to the cytologic features of cell cultures originating in invasive bladder carcinoma. PAS diastase showed double distribution and texture of the perinuclear glycosaminoglycans, a glossy accumulated mass and large granules. Glycosaminoglycan sacs similar to those of cell cultures were also present in tumor-dispersed cells. There was a nonspecific binding of antisera against lysozyme, human chorionic gonadotropin and alpha 1-trypsin in normal and tumor cells. Tumor cells and tissues were positive for alpha 1-chymotrypsin distributed perinuclearly and in large spheres. Normal cells lacked the above characteristics. The results indicate that it is feasible to use the aforementioned characteristics in conjunction with the existing bladder-cytologic criteria for malignancy as markers in urothelial cancer with regard to prognosis of superficial tumors with high malignant potential.
authors.affiliation: Department of Experimental Physiology, Medical School, University of Athens, Greece.
authors.forename: H
authors.initials: H
authors.lastname: Logothetou-Rella
authors.forename: A
authors.initials: A
authors.lastname: Karayiannis
authors.forename: E
authors.initials: E
authors.lastname: Vamvassakis
authors.forename: A
authors.initials: A
authors.lastname: Livaditou
authors.forename: D
authors.initials: D
authors.lastname: Pikramenos
authors.forename: J M
authors.initials: JM
authors.lastname: Nesland
authors.forename: C
authors.initials: C
authors.lastname: Dimopoulos
mesh_terms: D002295:Carcinoma, Transitional Cell / Q000737:chemistry / Q000648:ultrastructure*; D004721:Endoplasmic Reticulum / Q000648:ultrastructure; D006025:Glycosaminoglycans / Q000032:analysis; D006801:Humans; D007124:Immunoenzyme Techniques; D002479:Inclusion Bodies / Q000648:ultrastructure; D008854:Microscopy, Electron; D009361:Neoplasm Invasiveness; D013194:Staining and Labeling; D001743:Urinary Bladder / Q000648:ultrastructure*; D001749:Urinary Bladder Neoplasms / Q000737:chemistry / Q000648:ultrastructure*; D014556:Urine / Q000166:cytology
chemical_list: D006025:Glycosaminoglycans