Competitive binding assay for quantitative determination of GM1 ganglioside in plasma and cerebrospinal fluid.
A competitive binding assay for the quantitative determination of GM1 ganglioside is described. After extraction from biological fluids, GM1 was incubated with a known amount of cholera toxin B-subunit conjugated with horseradish peroxidase, and exposed to GM1 adsorbed onto polystyrene microwells. Since GM1 in solution blocks the binding of toxin B-subunit to GM1 adsorbed onto the solid phase, enzyme activity serves as a reciprocal measure of GM1 concentration in the sample. The assay was used to determine the basal level of GM1 in plasma and cerebrospinal fluid in different populations.
Kirschner
G
G
Fidia Research Laboratories, Abano Terme, Italy.
Bassan
M
M
Facco
M P
MP
Ferrari
G
G
Callegaro
L
L
D000328:Adult; D000368:Aged; D001667:Binding, Competitive; D002138:Calibration; D002772:Cholera Toxin; D005260:Female; D005312:Fetal Blood / Q000737:chemistry; D005677:G(M1) Ganglioside / Q000097:blood* / Q000134:cerebrospinal fluid*; D006735:Horseradish Peroxidase; D006801:Humans; D007124:Immunoenzyme Techniques; D011247:Pregnancy; D012016:Reference Values; D012680:Sensitivity and Specificity
C052635:cholera toxin, B subunit-horseradish peroxidase; D005677:G(M1) Ganglioside; D002772:Cholera Toxin; D006735:Horseradish Peroxidase