Inhibition of production of monocyte/macrophage-derived angiogenic activity by oxygen free-radical scavengers.
We showed previously that thiol-containing compounds inhibited the production of macrophage-mediated angiogenic activity. Since thiol-containing compounds may act on macrophages by affecting activation and inhibiting the production of oxygen free-radicals, we studied the effects of oxygen free-radical scavengers on production of angiogenic activity by elicited mouse peritoneal macrophages and lipopolysaccharide stimulated normal human monocytes. Monocyte/macrophage conditioned media were potently angiogenic when assayed in rat corneas, while conditioned media, from oxygen free-radical scavenger-treated cells were not. The inhibitory effect of oxygen free-radical scavengers was due to a direct effect on monocyte/macrophage production of angiogenic activity but was not due solely to a decrease in the production of the macrophage-derived angiogenic cytokine tumor necrosis factor-alpha. We conclude that oxygen free-radical scavengers are potent inhibitors of the production of macrophage-mediated angiogenic activity.
Koch
A E
AE
Department of Medicine, Northwestern University Medical School.
Cho
M
M
Burrows
J C
JC
Polverini
P J
PJ
Leibovich
S J
SJ
D043925:Angiogenesis Inducing Agents / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D000818:Animals; D001681:Biological Assay; D003315:Cornea / Q000098:blood supply; D003864:Depression, Chemical; D016166:Free Radical Scavengers; D006801:Humans; D007375:Interleukin-1 / Q000096:biosynthesis; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D009000:Monocytes / Q000187:drug effects* / Q000378:metabolism; D009389:Neovascularization, Pathologic / Q000517:prevention & control; D010100:Oxygen / Q000378:metabolism*; D014176:Protein Biosynthesis; D051381:Rats; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D043925:Angiogenesis Inducing Agents; D016166:Free Radical Scavengers; D007375:Interleukin-1; D014409:Tumor Necrosis Factor-alpha; D010100:Oxygen