[Photochemical transformation of Hoescht 33258 dye molecules complexed with cell nucleus DNA under the effect of UV-irradiation].
It is found that with time a decrease of fluorescence intensity of the basic band at 460 nm and appearance of a new band of fluorescence of DNA-specific dye Hoechst 33258 in complex with the cell nucleus DNA under the action of UV emission are observed. It is shown that phototransformation is related to the withdrawal of the nitrogen atom proton of piperazine ring in an excited state of the complex of the dye Hoechst 33258 with the cell nucleus DNA.
Umetskaia
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D000818:Animals; D006690:Bisbenzimidazole / Q000378:metabolism* / Q000528:radiation effects; D002467:Cell Nucleus / Q000378:metabolism*; D002478:Cells, Cultured; D004247:DNA / Q000378:metabolism*; D051379:Mice; D010777:Photochemistry; D013050:Spectrometry, Fluorescence; D013950:Thymus Gland / Q000166:cytology; D014466:Ultraviolet Rays
D004247:DNA; D006690:Bisbenzimidazole