[Limulus test (factor G) and polysaccharides from fungus].
Picogram quantifies per ml of endotoxin showed a positive limulus test. The concentration more than ten pg/ml of curdlan ((1-3)-beta-D-glucan) was also positive to a conventional limulus test, which contains a factor G. One nanogram per ml of CM-curdlan was positive, but higher concentration of 10 pg/ml of CM-curdlan decreased its optical density of the conventional limulus test. More than one hundred ng/ml of mannan from Saccharomyces cerevisiae did not react with factor G. However, a culture supernatant of Saccharomyces cerevisiae activated factor G. This result suggested that beta-glucan activated factor G. In addition, culture supernatant of Aspergillus fumigatus, Rhodotorula rubra, Candida parapsilosis, Candida tropicalis, Candida krusei also activated factor G, but Cryptococcus neoformans did not activate it.
Miyazaki
T
T
Second Department of Internal Medicine, Nagasaki University School of Medicine, Japan.
Kohno
S
S
Mitsutake
K
K
Maesaki
S
S
Yamada
H
H
Yasuoka
A
A
Sasayama
K
K
Kaku
M
M
Koga
H
H
Hara
K
K
D023181:Antimicrobial Cationic Peptides; D060829:Arthropod Proteins; D005658:Fungi / Q000378:metabolism*; D005936:Glucans / Q000378:metabolism; D007447:Invertebrate Hormones / Q000378:metabolism*; D008033:Limulus Test; D008351:Mannans / Q000378:metabolism; D011134:Polysaccharides / Q000378:metabolism*
D023181:Antimicrobial Cationic Peptides; D060829:Arthropod Proteins; D005936:Glucans; D007447:Invertebrate Hormones; D008351:Mannans; D011134:Polysaccharides; C034705:antilipopolysaccharide factor (Limulus)