Specific metaphase and interphase detection of the breakpoint region in 8q24 of Burkitt lymphoma cells by triple-color fluorescence in situ hybridization.
Triple fluorescence in situ hybridization with a plasmid DNA library from sorted human chromosomes 8 in combination with bacteriophage clones flanking the breakpoint in 8q24 of the Burkitt lymphoma cell line J1 was used for the specific delineation of this breakpoint in individual tumor cells. With this approach, tumor-specific breakpoints in translocation chromosomes can be detected at all stages of the cell cycle with high specificity.
Ried
T
T
Institute of Human Genetics and Anthropology, University of Heidelberg, Federal Republic of Germany.
Lengauer
C
C
Cremer
T
T
Wiegant
J
J
Raap
A K
AK
van der Ploeg
M
M
Groitl
P
P
Lipp
M
M
D002051:Burkitt Lymphoma / Q000235:genetics* / Q000473:pathology; D002871:Chromosome Banding; D002889:Chromosomes, Human, Pair 2 / Q000648:ultrastructure; D002898:Chromosomes, Human, Pair 8 / Q000648:ultrastructure*; D015342:DNA Probes; D005456:Fluorescent Dyes; D006801:Humans; D007399:Interphase; D008297:Male; D008677:Metaphase; D008856:Microscopy, Fluorescence; D009693:Nucleic Acid Hybridization; D009857:Oncogenes; D010957:Plasmids; D014178:Translocation, Genetic; D014407:Tumor Cells, Cultured / Q000648:ultrastructure
D015342:DNA Probes; D005456:Fluorescent Dyes