Association of red blood cell sodium leak with blood pressure in recombinant inbred strains.
Red blood cell Na+ content as well as ouabain-resistant Na+ and Rb+ (K+) transport (susceptible or resistant to inhibition by loop diuretics) were determined in spontaneously hypertensive rats (SHR) and normotensive Brown Norway (BN) rats the erythrocytes of which were incubated in either saline or Mg(2+)-sucrose medium. Elevated ouabain-resistant Na+ net uptake contrasted with slightly decreased red blood cell Na+ content in SHR compared with BN rats. Acceleration of furosemide- and bumetanide-sensitive Na+ fluxes contributed to enhanced ouabain-resistant Na+ influx into SHR erythrocytes in saline medium, whereas higher furosemide- or bumetanide-resistant Na+ efflux caused greater ouabain-resistant Na+ efflux in Mg(2+)-sucrose medium. Furosemide- and bumetanide-resistant Rb+ leaks were augmented in SHR erythrocytes. The association of the disclosed ion transport alterations with blood pressure was examined in 20 recombinant inbred strains derived from F2 SHR x BN hybrids. Ouabain-resistant Na+ uptake as well as furosemide- and bumetanide-resistant Na+ inward leaks (but not red blood cell Na+ content or furosemide- and bumetanide-sensitive Na+ net uptake) cosegregated with systolic and pulse pressures but not diastolic pressure of the recombinant inbred strains. In contrast, neither ouabain-resistant Na+ efflux nor any component of ouabain-resistant Rb+ uptake correlated positively with blood pressure of the recombinant inbred strains. Increased ouabain-resistant Na+ influx was compensated for by accelerated ouabain-sensitive Na+ extrusion because red blood cell Na+ content was not elevated in the hypertensive strains. Thus, high cell Na+ turnover rates might be related to genetic hypertension if an altered Na+ inward leak would be less effectively compensated for in tissues involved in cardiovascular regulation.
Talib
H K
HK
Institute of Physiology, Czechoslovak Academy of Sciences, Prague.
Dobesová
Z
Z
Klír
P
P
Kren
V
V
Kunes
J
J
Pravenec
M
M
Zicha
J
J
D000704:Analysis of Variance; D000818:Animals; D001692:Biological Transport; D001794:Blood Pressure / Q000187:drug effects / Q000235:genetics; D002034:Bumetanide / Q000494:pharmacology; D004912:Erythrocytes / Q000378:metabolism*; D005665:Furosemide / Q000494:pharmacology; D005819:Genetic Markers; D006824:Hybridization, Genetic; D006973:Hypertension / Q000503:physiopathology*; D008297:Male; D010042:Ouabain / Q000494:pharmacology; D011188:Potassium / Q000493:pharmacokinetics; D051381:Rats; D011914:Rats, Inbred BN; D011918:Rats, Inbred SHR; D012413:Rubidium / Q000493:pharmacokinetics; D012964:Sodium / Q000493:pharmacokinetics*
D005819:Genetic Markers; D002034:Bumetanide; D010042:Ouabain; D005665:Furosemide; D012964:Sodium; D012413:Rubidium; D011188:Potassium