Induction of endogenous retroviral gene product (SU) as an acute-phase protein by IL-6 in murine hepatocytes.
The effect of Lps locus and IL-6 on the production of SU (previously termed gp70), a mouse endogenous retroviral gene product, was studied. Back-cross studies using the progeny between (NZB x C3H/HeJ)F1 and C3H/HeJ mice indicate that the basal level of SU is not associated with the Lps locus on chromosome 4. Lipopolysaccharide (LPS) mitogen response-negative mice did not show the enhancement of serum SU production after LPS injection. Spleen cells from LPS-mitogen response-positive but not from negative mice showed increase of IL-6 synthesis in the presence of LPS. Since IL-6 may be involved in the production of serum SU, we tested the effect of IL-6 in a primary hepatocyte culture system. SU production was clearly enhanced in the presence of recombinant IL-6, indicating that IL-6 induced by LPS can enhance the expression of retroviral genome.
Itoh
Y
Y
Department of Molecular Pathology, Tokyo Metropolitan Institute of Gerontology, Japan.
Maruyama
N
N
Kitamura
M
M
Shirasawa
T
T
Shigemoto
K
K
Koike
T
T
D000209:Acute-Phase Proteins / Q000096:biosynthesis*; D000818:Animals; D002478:Cells, Cultured; D003433:Crosses, Genetic; D005260:Female; D015850:Interleukin-6 / Q000096:biosynthesis / Q000494:pharmacology*; D008070:Lipopolysaccharides; D008099:Liver / Q000378:metabolism*; D051379:Mice; D008809:Mice, Inbred C3H; D008811:Mice, Inbred DBA; D008814:Mice, Inbred NZB; D011994:Recombinant Proteins / Q000494:pharmacology; D015682:Retroviridae Proteins, Oncogenic / Q000096:biosynthesis*; D014158:Transcription, Genetic / Q000187:drug effects; D014759:Viral Envelope Proteins / Q000096:biosynthesis*
D000209:Acute-Phase Proteins; D015850:Interleukin-6; D008070:Lipopolysaccharides; D011994:Recombinant Proteins; D015682:Retroviridae Proteins, Oncogenic; D014759:Viral Envelope Proteins