Comparison of DNA strand break induction in CHO cells measured by alkaline elution and by fluorometric analysis of DNA unwinding (FADU).
DNA damage in X-irradiated CHO cells was measured by alkaline filter elution and compared to fluorometric analysis of DNA unwinding (FADU). The FADU method proved to be as sensitive as the alkaline filter elution technique in detecting X-ray induced DNA breaks. Strand break induction was also measured after treatment with four radical generating chemicals (hydrogen peroxide, bleomycin, mitomycin C and methyl viologen) using the FADU technique.
Baumstark-Khan
C
C
Radiologische Universitätsklinik, Experimentelle Radiologie und Strahlenbiologie, Bonn, Germany.
Griesenbach
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U
Rink
H
H
D000818:Animals; D001761:Bleomycin / Q000494:pharmacology; D016466:CHO Cells / Q000187:drug effects / Q000528:radiation effects; D006224:Cricetinae; D004247:DNA / Q000187:drug effects / Q000528:radiation effects; D004249:DNA Damage; D004277:DNA, Single-Stranded / Q000032:analysis; D006861:Hydrogen Peroxide / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D016685:Mitomycin / Q000494:pharmacology; D009690:Nucleic Acid Conformation; D009691:Nucleic Acid Denaturation; D010269:Paraquat / Q000494:pharmacology; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence; D014462:Ultrafiltration
D004277:DNA, Single-Stranded; D001761:Bleomycin; D016685:Mitomycin; D004247:DNA; D006861:Hydrogen Peroxide; D010269:Paraquat