Influence of cinnamaldehyde on UV-induced gene conversion and point mutation in yeast: effect on protein synthesis.
The activity of cinnamaldehyde (CIN), a bioantimutagen in bacterial systems, was tested in the D7 strain of yeast Saccharomyces cerevisiae. Yeast cells were UV-irradiated and post-incubated in liquid growth medium for 2 and 4 h with different concentrations of cinnamaldehyde. During the post-incubation period, DNA-damage-specific functions may be induced. This in turn may affect the genotoxicity and in fact a weak decrease in UV-induced convertant and revertant frequencies was observed after 4 h of post-incubation. The presence of CIN in the growth medium increased the UV-induced gene conversion and reversion. The addition of cycloheximide abolished this effect. To evaluate the CIN effect on protein synthesis, extracts of cells UV-treated and post-incubated for 2 h in the presence of 35S-methionine were performed. SDS-gel electrophoresis demonstrated the inhibitory effect of CIN on a UV-specific protein. This work suggests that CIN might interfere with DNA-damage-inducible systems although it did not exert an antimutagenic activity in our experimental conditions.
Galli
A
A
Istituto di Mutagenesi e Differenziamento CNR, Pisa, Italy.
Della Croce
C
C
Minnucci
S
S
Fiorio
R
R
Bronzetti
G
G
D000171:Acrolein / Q000031:analogs & derivatives* / Q000494:pharmacology; D003513:Cycloheximide / Q000494:pharmacology; D005656:Fungal Proteins / Q000096:biosynthesis; D009154:Mutation; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000528:radiation effects; D014466:Ultraviolet Rays
D005656:Fungal Proteins; D000171:Acrolein; D003513:Cycloheximide; C012843:cinnamaldehyde