Unicolor and bicolor in situ hybridization in the diagnosis of peripheral neuroepithelioma and related tumors.
The chromosome 22 breakpoint of the t(11;22) translocation of peripheral neuroepithelioma has been located, by fluorescence in situ hybridization, in the proximity of the interface between 22q12.1 and 22q12.2. Use of single cosmids or pools of cosmids of the flanking regions enables the monitoring of the translocation in interphase and in metaphase chromosomes. This method can now be routinely applied for the diagnosis of this translocation in mixed round cell tumors.
Desmaze
C
C
URA 620 CNRS, Paris, France.
Zucman
J
J
Delattre
O
O
Thomas
G
G
Aurias
A
A
D001859:Bone Neoplasms / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology; D002871:Chromosome Banding; D002880:Chromosomes, Human, Pair 11 / Q000648:ultrastructure*; D002892:Chromosomes, Human, Pair 22 / Q000648:ultrastructure*; D003116:Color; D003360:Cosmids; D015342:DNA Probes; D005819:Genetic Markers; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D018241:Neuroectodermal Tumors, Primitive, Peripheral / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology; D010524:Peripheral Nervous System Neoplasms / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology; D012512:Sarcoma, Ewing / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology; D014178:Translocation, Genetic
D015342:DNA Probes; D005819:Genetic Markers