Time course of serotonergic afferent plasticity within rat spinal trigeminal nucleus following infraorbital nerve transection.
High-performance liquid chromatography with electrochemical detection (HPLC-ED) and immunocytochemistry were used to examine the time course of serotonergic afferent plasticity within trigeminal subnucleus interpolaris (SpVi) following infraorbital nerve (ION) transection in adult rats. Biochemical analysis was also performed in trigeminal subnucleus caudalis (SpVc) to examine the possibility of transient lesion-induced changes in this region. No significant changes in serotonin (5-HT) or 5-hydroxyindoleacetic acid (5-HIAA) concentration, or in density of 5-HT-immunoreactive (5-HTIR) axonal varicosities were observed in either subnucleus on the lesioned side, up to 51 days following ION cut. However, at 76-79 days post-lesion, a significant increase in 5-HT concentration was again demonstrated within SpVi.
Klein
B G
BG
Department of Biomedical Sciences, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg 24061.
Blaker
W D
WD
White
C F
CF
Misra
B R
BR
D000818:Animals; D001923:Brain Chemistry / Q000502:physiology; D004563:Electrochemistry; D006897:Hydroxyindoleacetic Acid / Q000378:metabolism; D008297:Male; D009473:Neuronal Plasticity / Q000502:physiology*; D009475:Neurons, Afferent / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012701:Serotonin / Q000502:physiology*; D013116:Spinal Cord / Q000166:cytology / Q000502:physiology*; D014276:Trigeminal Nerve / Q000166:cytology / Q000502:physiology*
D012701:Serotonin; D006897:Hydroxyindoleacetic Acid