Stimulation of adenylate cyclase by amylin in CHO-K1 cells.
The CHO-K1 cell line responds to the peptide amylin by a rapid elevation of cAMP. The related peptide calcitonin gene-related peptide (CGRP) is 100 times less potent at stimulating adenylate cyclase than is amylin. The actions of amylin at this receptor are concentration dependent and not antagonized by the CGRP antagonist CGRP-(8-37). Although these cells have receptors for calcitonin, amylin is unable to take part in any high affinity interaction with these receptors, as assessed by radioligand binding. The CHO-K1 cell line has receptors for amylin that are distinct from those for calcitonin and CGRP.
D'Santos
C S
CS
Medical Research Council Centre, Cambridge, UK.
Gatti
A
A
Poyner
D R
DR
Hanley
M R
MR
D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D000262:Adenylyl Cyclases / Q000378:metabolism*; D000682:Amyloid / Q000494:pharmacology*; D000818:Animals; D016466:CHO Cells; D002116:Calcitonin / Q000378:metabolism / Q000494:pharmacology; D015740:Calcitonin Gene-Related Peptide / Q000494:pharmacology; D006224:Cricetinae; D000242:Cyclic AMP / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation; D006801:Humans; D058228:Islet Amyloid Polypeptide; D007700:Kinetics; D018003:Receptors, Calcitonin; D011956:Receptors, Cell Surface / Q000378:metabolism
D000682:Amyloid; D058228:Islet Amyloid Polypeptide; D018003:Receptors, Calcitonin; D011956:Receptors, Cell Surface; D002116:Calcitonin; D000242:Cyclic AMP; D000262:Adenylyl Cyclases; D015740:Calcitonin Gene-Related Peptide; D015056:1-Methyl-3-isobutylxanthine