Analysis of islet cell antibodies reactivity to a human islet cell line.
A human pancreatic beta cell line (HP62) was tested for reactivity with islet cell antibodies (ICA) as compared with previously-established methods. Using indirect immunofluorescence test, we found that HP62 cell line failed to react in a specific way with ICA from type 1 (insulin-dependent) diabetic patients since sera from normal controls showed a reactivity similar to that found in the patients. So, the usefulness of this human beta cell line as a tool of immunological purpose is questioned when indirect immunofluorescence procedures are used.
Muñoz
A
A
Endocrinology and Diabetes Unit, Hospital Clinic, Barcelona, Spain.
Gallart
T
T
Gomis
R
R
D001323:Autoantibodies / Q000276:immunology*; D002460:Cell Line; D003922:Diabetes Mellitus, Type 1 / Q000276:immunology*; D000939:Epitopes / Q000276:immunology; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D007515:Islets of Langerhans / Q000276:immunology*; D008297:Male
D001323:Autoantibodies; D000939:Epitopes; C024211:islet cell antibody