title: Assessment of proliferative activity in ovarian neoplasms by flow and static cytometry. Correlation with prognostic features.
abstract: We undertook a prospective flow and static cytometric study of proliferative activity in 74 malignant, borderline and benign ovarian neoplasms. Proliferative activity as assessed by S phase fraction (SPF), and immunostaining of tissue sections with Ki-67 antibody was compared with prognostically important clinicopathologic features. Malignant neoplasms had higher median percentage Ki-67 staining (27.6%) than borderline (12.3%) and benign (2.7%) tumors (P less than 0.05). Percentage Ki-67 staining correlated with SPF, DNA index, architectural grade, nucleolar grade, and mitotic count in malignant tumors; with nucleolar grade in benign tumors and with none of these variables in borderline tumors. Similarly, malignant neoplasms had a higher median SPF (11.5%) than borderline (3.4%) and benign (2.9%) neoplasms (P less than 0.05). In malignant neoplasms, SPF correlated with percentage Ki-67 staining, DNA index, age, and stage, but with none of these in borderline or benign neoplasms.
authors.affiliation: Department of Pathology, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts.
authors.forename: P C
authors.initials: PC
authors.lastname: Huettner
authors.forename: D S
authors.initials: DS
authors.lastname: Weinberg
authors.forename: J M
authors.initials: JM
authors.lastname: Lage
mesh_terms: D000368:Aged; D002455:Cell Division; D002467:Cell Nucleus / Q000378:metabolism; D003584:Cytological Techniques; D004247:DNA / Q000235:genetics; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007124:Immunoenzyme Techniques; D019394:Ki-67 Antigen; D008875:Middle Aged; D009687:Nuclear Proteins / Q000032:analysis; D010051:Ovarian Neoplasms / Q000378:metabolism / Q000473:pathology*; D011003:Ploidies; D011379:Prognosis; D016196:S Phase; D013194:Staining and Labeling
chemical_list: D019394:Ki-67 Antigen; D009687:Nuclear Proteins; D004247:DNA