Alzheimer disease brain extract stimulates branching of laminin-mediated neuronal processes.
Patients with Alzheimer disease (AD) suffer mental deterioration associated with neurofibrillary tangle and senile plaque formation in the brain. Here we have determined the effects of brain extracts from normal and from AD patients on neuronal process formation by a pheochromocytoma (PC-12) and a neuroblastoma x glioma hybrid cell line (NG108-15). PC12 cells show a dose-related stimulation of branching of neuronal processes by AD brain extracts with cells cultured on a laminin substrate. The neurotrophic effects of extracts of AD brains may be related to the abnormal sprouting and neurofibrillary tangle formation observed in the brain in this disorder.
Kittur
S D
SD
Gerontology Research Center, National Institute on Aging, National Institutes of Health, Baltimore, Maryland.
Endo
H
H
Adler
W H
WH
Martin
G R
GR
Markesbery
W R
WR
Kleinman
H K
HK
Weeks
B S
BS
D000310:Adrenal Gland Neoplasms; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000503:physiopathology*; D001921:Brain / Q000503:physiopathology*; D002460:Cell Line; D005260:Female; D005910:Glioma; D006801:Humans; D007797:Laminin / Q000502:physiology*; D008297:Male; D008875:Middle Aged; D008970:Molecular Weight; D009416:Nerve Regeneration / Q000502:physiology*; D009419:Nerve Tissue Proteins / Q000502:physiology; D009447:Neuroblastoma; D009454:Neurofibrils / Q000502:physiology*; D009473:Neuronal Plasticity / Q000502:physiology*; D010673:Pheochromocytoma; D014020:Tissue Extracts / Q000502:physiology*
D007797:Laminin; D009419:Nerve Tissue Proteins; D014020:Tissue Extracts