Production of anti-peptide specific antibody in mice following immunization with peptides conjugated to mannan.
In order to investigate the usefulness of polysaccharides as carriers for the induction of antibody to synthetic peptides, peptides representing residues 139-147 of the surface antigen of hepatitis B and residues 129-140 of the pre-S2 region of the protein were coupled to mannan and dextran via an aminocaproic spacer molecule. Of the two conjugates studied, only mannan was useful as a carrier for the efficient production of anti-peptide antibodies.
Okawa
Y
Y
Department of Clinical Sciences, London School of Hygiene and Tropical Medicine, UK.
Howard
C R
CR
Steward
M W
MW
D000614:Aminocaproates; D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000096:biosynthesis*; D000918:Antibody Specificity; D003911:Dextrans / Q000276:immunology; D004306:Dose-Response Relationship, Immunologic; D004797:Enzyme-Linked Immunosorbent Assay; D006514:Hepatitis B Surface Antigens; D000922:Immunotoxins / Q000276:immunology*; D008297:Male; D008351:Mannans / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C
D000614:Aminocaproates; D000888:Antibodies, Anti-Idiotypic; D003911:Dextrans; D006514:Hepatitis B Surface Antigens; D000922:Immunotoxins; D008351:Mannans