C/EBP and c-JUN proteins activate the proximal enhancer of the developmentally regulated alpha-fetoprotein gene.
The expression of the alpha-fetoprotein (AFP) gene is developmentally regulated. Active transcription of this gene depends on a proximal enhancer sequence located between positions D-203 bp and -81 bp, upstream the initiation site. This enhancer contains several putative binding sites for transcription factors. By transfection experiments, we showed that the enhancer activity can be driven by interactions with two regulatory factors, namely C/EBP and c-JUN.
Houart
C
C
Université Libre de Bruxelles, Département of Molecular Biology, Rhode-St-Genèse, Belgium.
Szpirer
J
J
Szpirer
C
C
D001483:Base Sequence; D022762:CCAAT-Enhancer-Binding Proteins; D002460:Cell Line; D004268:DNA-Binding Proteins / Q000502:physiology*; D004742:Enhancer Elements, Genetic; D005786:Gene Expression Regulation; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000502:physiology*; D010957:Plasmids; D016755:Proto-Oncogene Proteins c-jun / Q000502:physiology*; D014157:Transcription Factors; D000509:alpha-Fetoproteins / Q000235:genetics*
D022762:CCAAT-Enhancer-Binding Proteins; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D016755:Proto-Oncogene Proteins c-jun; D014157:Transcription Factors; D000509:alpha-Fetoproteins