The NH2 terminus of retinal recoverin is acylated by a small family of fatty acids.
Recoverin is a recently identified Ca(2+)-binding protein that imparts Ca2+ sensitivity to vertebrate photoreceptor guanylate cyclase. In response to photo-induced depletion of intracellular cGMP and Ca2+, recoverin stimulates resynthesis of cGMP. Bovine retinal recoverin has now been analyzed by electrospray mass spectrometry (ESI-MS) for post-translational modifications that might influence its activity. Heterogeneous acylation was detected at the NH2 terminus of bovine retinal recoverin. The NH2-terminal glycine of each retinal recoverin molecule is linked to one of four different types of acyl groups. The most abundant is myristoleate (14:1), but 14:0, 14:2, and 12:0 acyl residues are also present.
Dizhoor
A M
AM
A. N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Russia.
Ericsson
L H
LH
Johnson
R S
RS
Kumar
S
S
Olshevskaya
E
E
Zozulya
S
S
Neubert
T A
TA
Stryer
L
L
Hurley
J B
JB
Walsh
K A
KA
D000215:Acylation; D000595:Amino Acid Sequence; D000818:Animals; D002135:Calcium-Binding Proteins / Q000302:isolation & purification / Q000378:metabolism*; D002417:Cattle; D002849:Chromatography, Gas; D005136:Eye Proteins; D051597:Hippocalcin; D008074:Lipoproteins; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D008970:Molecular Weight; D019814:Myristic Acid; D009227:Myristic Acids / Q000378:metabolism*; D009419:Nerve Tissue Proteins; D010446:Peptide Fragments / Q000302:isolation & purification; D011994:Recombinant Proteins / Q000378:metabolism; D051603:Recoverin; D012374:Rod Cell Outer Segment / Q000378:metabolism*; D014357:Trypsin
D002135:Calcium-Binding Proteins; D005136:Eye Proteins; D008074:Lipoproteins; D009227:Myristic Acids; D009419:Nerve Tissue Proteins; D010446:Peptide Fragments; D011994:Recombinant Proteins; D019814:Myristic Acid; D051603:Recoverin; D051597:Hippocalcin; D014357:Trypsin