title: 2H-nuclear magnetic resonance imaging of tumor blood flow: spatial and temporal heterogeneity in a tissue-isolated mammary adenocarcinoma.
abstract: 2H-Nuclear magnetic resonance imaging of deuteron accumulation in tissue following an i.v. bolus of deuterium oxide provides a noninvasive means of constructing maps of tissue perfusion. With a measured arterial input function and a simple model for tissue-capillary exchange, these data can provide quantitative estimates of local flow. This technique was tested in rat brain and then applied to the study of spatial heterogeneity and temporal variation of blood flow in the tissue-isolated R3230AC mammary adenocarcinoma. Global flow from the brain averaged 0.96 ml/min.g, in good agreement with results obtained from other methods; the perfusion of brain was relatively homogeneous. Global tumor blood flow averaged 0.32 ml/min.g, ranging from 0.11 to 0.96 ml/min.g. Imaging revealed variations in perfusion both within and between the tumors that far exceeded those expected from brain flow heterogeneity and uncertainty in the flow estimates. By obtaining repeated flow images at 30-min intervals, it was possible to show that the regional blood flow shifted with time in single pixels and in multipixel regions. These experiments show that 2H-nuclear magnetic resonance may be useful in obtaining noninvasive and quantitative measurement of temporal blood flow changes in a solid tumor in vivo.
authors.affiliation: Department of Chemical Engineering, Carnegie Mellon University, Pittsburgh, Pennsylvania 15213.
authors.forename: C J
authors.initials: CJ
authors.lastname: Eskey
authors.forename: A P
authors.initials: AP
authors.lastname: Koretsky
authors.forename: M M
authors.initials: MM
authors.lastname: Domach
authors.forename: R K
authors.initials: RK
authors.lastname: Jain
mesh_terms: D000230:Adenocarcinoma / Q000098:blood supply*; D000818:Animals; D001158:Arteries / Q000502:physiology; D002196:Capillaries / Q000502:physiology; D002560:Cerebrovascular Circulation; D003903:Deuterium / Q000493:pharmacokinetics; D009682:Magnetic Resonance Spectroscopy; D008325:Mammary Neoplasms, Experimental / Q000098:blood supply*; D051381:Rats; D011916:Rats, Inbred F344; D012039:Regional Blood Flow
chemical_list: D003903:Deuterium