The effect of gamma-irradiation on the adherent capacity and iron metabolism of alveolar macrophages in mice and rats.
The effect of external gamma-irradiation on the survival of alveolar macrophages (AM) and on the extracellular release of 59Fe from AM-ingested [59Fe]iron hydroxide colloid was investigated in vitro using cells from C3H mice and Wistar rats. 59Fe release from mouse AM was enhanced by irradiation in a dose-dependent fashion, but irradiation up to 112 Gy did not affect the release from rat AM. Cell survival of AM, measured by direct counting of cell nuclei of adherent AM or vital staining with crystal violet, decreased dose dependently in mice and rats, but mouse AM were more radiosensitive than rat AM.
Kubota
Y
Y
Division of Comparative Radiotoxicology, National Institute of Radiological Sciences, Chiba, Japan.
Sato
H
H
Takahashi
S
S
D000818:Animals; D002448:Cell Adhesion / Q000502:physiology / Q000528:radiation effects*; D005720:Gamma Rays; D007501:Iron / Q000378:metabolism / Q000528:radiation effects*; D016676:Macrophages, Alveolar / Q000378:metabolism / Q000502:physiology / Q000528:radiation effects*; D051379:Mice; D008809:Mice, Inbred C3H; D051381:Rats; D017208:Rats, Wistar; D013045:Species Specificity
D007501:Iron