Endotoxin removed from hemoglobin solution using polymyxin-B immobilized fibre (PMX-F) followed by a new turbidometric endotoxin assay.
Endotoxin contamination in modified hemoglobin can result in side effects. Accurate measurement and effective elimination of endotoxin are important in producing safe hemoglobin preparation. A new turbidometric endotoxin assay (Toxinometer) was studied, in which absorbance of wavelength was applied at 660 nm. This method is not affected by the presence of hemoglobin in solution. This way, toxinometer can accurately measure endotoxin concentration in hemoglobin solution. For the elimination of endotoxin, polymyxin-B immobilized fiber (PMX-F) was studied in-vitro and compared with commercial materials. The PMX-F was found to be a convenient and less expensive approach.
Tani
T
T
First Department of Surgery, Shiga University of Medical Science, Nippon.
Chang
T M
TM
Kodama
M
M
Tsuchiya
M
M
D000818:Animals; D001802:Blood Substitutes / Q000302:isolation & purification*; D002417:Cattle; D004340:Drug Contamination; D004731:Endotoxins / Q000032:analysis / Q000302:isolation & purification*; D006454:Hemoglobins / Q000302:isolation & purification*; D009391:Nephelometry and Turbidimetry; D011112:Polymyxin B; D013053:Spectrophotometry
D001802:Blood Substitutes; D004731:Endotoxins; D006454:Hemoglobins; D011112:Polymyxin B