[Morpho-biochemical study of catecholamine metabolism in the myocardium of dogs with fibrillation and autolysis].
The balance of catecholamines (CA) and their metabolites in the canine heart during myocardial fibrillation and 1, 2, 3, 4, 5, 6 hours after death was studied. The fibrillation was caused by low myocardial electrostimulation. The comparative neuro-morphometric analysis of the CA-containing structures in 1 and 5 min of the fibrillation and the determination of the norepinephrine, its precursors and metabolites by HPLC-ED method was done. There was an increase of the myocardial norepinephrine (20%), 3-4-dihydroxyphenylethyleneglycol (25%) and decrease of the dopamine (50%) contents without any qualitative changes of CA metabolism. Autolysis leads to the successive decrease of the norepinephrine and dopamine contents without any qualitative changes of the CA metabolism too. These data suggest that autopsies could be used for the determination of the myocardial CA content and the qualitative characteristics of the CA metabolism before death in those who died suddenly.
Anikin
A Iu
AIu
Smirnova
E P
EP
Pashentseva
G A
GA
D000818:Animals; D001329:Autolysis; D001344:Autopsy; D002395:Catecholamines / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D003645:Death, Sudden / Q000473:pathology; D004285:Dogs; D004298:Dopamine / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism* / Q000473:pathology; D009638:Norepinephrine / Q000378:metabolism; D013997:Time Factors; D014693:Ventricular Fibrillation / Q000378:metabolism* / Q000473:pathology
D002395:Catecholamines; D004298:Dopamine; D009638:Norepinephrine