Class II (I-Ad) restricted T-cell recognition of the V3 loop region of HIV-1 gp120.
A CD4+ and gp120-specific T-cell line, and subclone, were established from BALB/c mice following immunization with recombinant gp120, using an adjuvant formulation (alum) acceptable for use in the human. The recognition specificity was determined against a panel of synthetic peptides corresponding to the primary sequence of the HXB2 strain of human immunodeficiency virus (HIV). An epitope was identified, corresponding to amino acid residues 322-341, and a further series of truncated peptides established the minimum determinant to be 327-341. Possible reasons for the immunodominance of the V3 loop, and adjacent regions, for both antibody and T-cell recognition are discussed.
Warren
A P
AP
National Institute for Medical Research, Mill Hill, London, England.
Thomas
D B
DB
D000595:Amino Acid Sequence; D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D000939:Epitopes / Q000276:immunology; D015699:HIV Envelope Protein gp120 / Q000276:immunology*; D015497:HIV-1 / Q000276:immunology*; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D010446:Peptide Fragments / Q000276:immunology*
D000939:Epitopes; D015699:HIV Envelope Protein gp120; C059762:HIV envelope protein gp120 (305-321); D000949:Histocompatibility Antigens Class II; D010446:Peptide Fragments