title: Molecular cloning of a novel hyaluronan receptor that mediates tumor cell motility.
abstract: A cDNA encoding a unique hyaluronan receptor has been molecularly cloned from a lambda GT11 3T3 cDNA expression library. Immunoblot analyses of cell lysates, using antibodies to peptides encoded in the cDNA, specifically react with a 58-kD protein. This protein is regulated by the mutant H-ras gene in cells containing a metallothionein promoter H-ras hybrid gene. Further, antibodies to peptide sequences encoded in the cDNA block the increase in locomotion resulting from induction of the mutant H-ras gene in this cell line. In a transblot assay, the bacterially expressed protein binds to biotinylated hyaluronan. Antibodies to peptides encoded in the cDNA react in immunoblot assays with the 58- and 52-kD proteins of a novel hyaluronan receptor complex previously implicated in cell locomotion. Furthermore, antibodies specific to the 58- and 52-kD proteins, which block ras-induced locomotion, also cross-react with the expressed, encoded protein. The gene product described here appears to be a new type of hyaluronan receptor that is involved in cell locomotion. It is named RHAMM, an acronym for receptor for hyaluronan-mediated motility.
authors.affiliation: Department of Biochemistry, University of Alabama, Birmingham 35233.
authors.forename: C
authors.initials: C
authors.lastname: Hardwick
authors.forename: K
authors.initials: K
authors.lastname: Hoare
authors.forename: R
authors.initials: R
authors.lastname: Owens
authors.forename: H P
authors.initials: HP
authors.lastname: Hohn
authors.forename: M
authors.initials: M
authors.lastname: Hook
authors.forename: D
authors.initials: D
authors.lastname: Moore
authors.forename: V
authors.initials: V
authors.lastname: Cripps
authors.forename: L
authors.initials: L
authors.lastname: Austen
authors.forename: D M
authors.initials: DM
authors.lastname: Nance
authors.forename: E A
authors.initials: EA
authors.lastname: Turley
mesh_terms: D016475:3T3 Cells; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002465:Cell Movement; D003001:Cloning, Molecular; D004247:DNA; D004926:Escherichia coli / Q000235:genetics; D005455:Fluorescent Antibody Technique; D005786:Gene Expression Regulation; D011905:Genes, ras; D018960:Hyaluronan Receptors; D006820:Hyaluronic Acid; D051379:Mice; D008969:Molecular Sequence Data; D011956:Receptors, Cell Surface / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D015183:Restriction Mapping; D014407:Tumor Cells, Cultured
chemical_list: D018960:Hyaluronan Receptors; D011956:Receptors, Cell Surface; D006820:Hyaluronic Acid; D004247:DNA