title: In vitro synthesis of bacterio-opsin: integration into microsomal membranes.
abstract: The translation and membrane integration of bacterio-opsin from Halobacterium salinarium were investigated. Plasmids containing the bacterio-opsin-gene with or without its original presequence were transcribed with the T7-RNA-polymerase and translated in vitro in a wheat germ system. The integration of the expressed bacterio-opsin into dog pancreas microsomes was studied. Both precursor bacterio-opsin and mature bacterio-opsin integrate into the eukaryotic membrane.
authors.affiliation: Freie Universität Berlin, FB Physik, AG Biophysik, Germany.
authors.forename: U
authors.initials: U
authors.lastname: Bauer
authors.forename: V
authors.initials: V
authors.lastname: Hildebrandt
authors.forename: N A
authors.initials: NA
authors.lastname: Dencher
authors.forename: P
authors.initials: P
authors.lastname: Wrede
mesh_terms: D000818:Animals; D001436:Bacteriorhodopsins / Q000378:metabolism*; D002417:Cattle; D015870:Gene Expression; D006217:Halobacterium; D066298:In Vitro Techniques; D008565:Membrane Proteins / Q000378:metabolism; D008861:Microsomes / Q000378:metabolism*; D014176:Protein Biosynthesis; D011499:Protein Processing, Post-Translational; D012329:RNA, Bacterial / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism
chemical_list: D008565:Membrane Proteins; D012329:RNA, Bacterial; D012333:RNA, Messenger; D001436:Bacteriorhodopsins; C017827:bacterio-opsin