Efficient purification of PCR products using ultrafiltration.
The use of disposable ultrafiltration devices for the purification of PCR-amplified DNA products from the other components of the reaction mixture is outlined. The advantages of using a 100,000 molecular weight cutoff membrane are discussed. The integrity of the membrane-purified DNA is checked by its successful use in sequencing, ligation and cloning procedures.
Krowczynska
A M
AM
Amicon, Inc., Beverly, MA 01915-1065.
Henderson
M B
MB
D004247:DNA / Q000302:isolation & purification*; D004279:DNA, Viral / Q000302:isolation & purification; D016133:Polymerase Chain Reaction / Q000379:methods*; D014462:Ultrafiltration / Q000295:instrumentation
D004279:DNA, Viral; D004247:DNA