title: The chicken neural extracellular matrix molecule restrictin: similarity with EGF-, fibronectin type III-, and fibrinogen-like motifs.
abstract: Restrictin is a chick neural extracellular matrix protein implicated in neural cell attachment and found to be associated with the cell surface recognition protein F11. Here we show by cDNA cloning that restrictin is a large multidomain protein composed of 4 structural motifs. At the N-terminus restrictin contains a cysteine-rich segment of about 140 aa that might link restrictin monomers into oligomers. This region is followed by 4.5 epidermal growth factor-like repeats and then by 9 consecutive motifs that are similar to fibronectin type III motifs. At the C-terminus restriction is related to the beta and gamma chains of fibrinogen, including similarity to a calcium-binding segment. Restrictin shows substantial sequence similarity with tenascin (cytotactin) throughout the polypeptide, and like tenascin, it forms oligomeric structures, as revealed by electron microscopy of immunoaffinity-purified restriction. The cell attachment site of restrictin is mapped to the C-terminal region by antibody perturbation experiments.
authors.affiliation: Zentrum für Molekulare Neurobiologie, Hamburg, Germany.
authors.forename: U
authors.initials: U
authors.lastname: Nörenberg
authors.forename: H
authors.initials: H
authors.lastname: Wille
authors.forename: J M
authors.initials: JM
authors.lastname: Wolff
authors.forename: R
authors.initials: R
authors.lastname: Frank
authors.forename: F G
authors.initials: FG
authors.lastname: Rathjen
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001923:Brain Chemistry; D002448:Cell Adhesion; D015816:Cell Adhesion Molecules, Neuronal / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D002645:Chickens; D003001:Cloning, Molecular; D004247:DNA / Q000737:chemistry; D004815:Epidermal Growth Factor / Q000737:chemistry*; D005109:Extracellular Matrix / Q000737:chemistry*; D016326:Extracellular Matrix Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D005340:Fibrinogen / Q000737:chemistry*; D005353:Fibronectins / Q000737:chemistry*; D046911:Macromolecular Substances; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D015394:Molecular Structure; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D019063:Tenascin
chemical_list: D015816:Cell Adhesion Molecules, Neuronal; D016326:Extracellular Matrix Proteins; D005353:Fibronectins; D046911:Macromolecular Substances; D019063:Tenascin; C074638:tenascin R; D004815:Epidermal Growth Factor; D005340:Fibrinogen; D004247:DNA