A novel ganglioside expressed by mouse hematopoietic cell lines.
Mouse progenitor T cell-derived cell lines were established by fusion of cells of hematopoietic organs such as bone marrow and fetal liver with T lymphoma (BW5147) to determine their characteristic cell-surface components. The hybridomas with the phenotype of Thy-1+, CD3-, CD4-, CD8- and expression of T cell receptor gene mRNA (BM216 and FL339) were selected for progenitor T cell-derived cell lines, and their ganglioside compositions were studied. A ganglioside component with a mobility slightly faster than that of bovine brain GD1a on high-performance thin-layer chromatography was found in the cell extracts of these cell lines as one of the most abundant components and was absent in the extract of the parental cell line (BW5147). The structure of the ganglioside was determined to be: NeuAc alpha-Gal beta-Gal beta-Gal alpha-Gal beta-Glc beta-ceramide. Gangliosides with such a sequence have never been found before, suggesting the possibility that the ganglioside is expressed as a surface marker of the cells in hematopoietic organs committed to a specific cell lineage, presumably to T cell lineage. cells in hematopoietic organs committed to a specific
Shimamura
M
M
Laboratory of Molecular Immunology, Mitsubishi Kasei Institute of Life Sciences, Tokyo, Japan.
Oku
M
M
Yamagata
T
T
D000818:Animals; D015152:Blotting, Northern; D002240:Carbohydrate Sequence; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D005732:Gangliosides / Q000235:genetics / Q000378:metabolism*; D006028:Glycosphingolipids / Q000032:analysis; D006412:Hematopoietic Stem Cells / Q000378:metabolism*; D006825:Hybridomas; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000235:genetics; D014158:Transcription, Genetic
D005732:Gangliosides; D006028:Glycosphingolipids; D016692:Receptors, Antigen, T-Cell, gamma-delta