title: Similarity of copper and technetium binding sites in human IgG.
abstract: Kits for direct labeling of IgG with 99mTc were used without modification for the preparation of [67Cu]IgG. The IgG was pre-treated to generate thiolate groups which would bind 67Cu. The direct labeling of reduced IgG with 67Cu was highly efficient, resulting in approx. 95% 67Cu binding. Non-reduced IgG (negative control) had labeling efficiencies of less than 10%. IgG pre-exposed to Cu(II) had reduced amounts of 99mTc bound to it. The results demonstrate a direct relationship between copper- and 99mTc-binding sites in IgG.
authors.affiliation: RhoMed Inc., Albuquerque, NM 87109.
authors.forename: P O
authors.initials: PO
authors.lastname: Zamora
authors.forename: J A
authors.initials: JA
authors.lastname: Mercer-Smith
authors.forename: M J
authors.initials: MJ
authors.lastname: Marek
authors.forename: L D
authors.initials: LD
authors.lastname: Schulte
authors.forename: B A
authors.initials: BA
authors.lastname: Rhodes
mesh_terms: D001665:Binding Sites; D001667:Binding, Competitive; D002851:Chromatography, High Pressure Liquid; D003300:Copper / Q000378:metabolism*; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism*; D013667:Technetium / Q000378:metabolism*; D005719:gamma-Globulins / Q000378:metabolism
chemical_list: D007074:Immunoglobulin G; D005719:gamma-Globulins; D013667:Technetium; D003300:Copper