Regulation of T cell proliferation by anti-CD49d and anti-CD29 monoclonal antibodies.
The beta 1 integrin VLA-4 (alpha 4 beta 1, CD49d/CD29), which is expressed on a large subpopulation of peripheral blood T lymphocytes, functions as a receptor for the endothelial adhesion protein VCAM-1 and the extracellular matrix protein fibronectin. Previous studies showed that immobilized fibronectin enhanced anti-CD3 monoclonal antibody (mAb)-induced T cell proliferation through binding to the integrins VLA-4 and VLA-5 (alpha 5 beta 1, CD49e/CD29). We studied the ability of the anti-CD49d mAb L25 to potentiate proliferation. T cell proliferation was induced by subthreshold concentrations of anti-CD3 mAb (mAb OKT3) coimmobilized with mAb L25 but not with coimmobilized anti-CD29 (beta 1) mAb. Soluble anti-CD29 mAb inhibited the proliferation induced by coimmobilized mAb OKT3 and L25 but not proliferation induced by mAb OKT3 with PMA or coimmobilized anti-CD26 mAb.
Bednarczyk
J L
JL
Department of Immunology, University of Texas M.D. Anderson Cancer Center, Houston 77030.
Teague
T K
TK
Wygant
J N
JN
Davis
L S
LS
Lipsky
P E
PE
McIntyre
B W
BW
D015703:Antigens, CD / Q000276:immunology*; D017252:CD3 Complex / Q000276:immunology; D002448:Cell Adhesion / Q000502:physiology; D005353:Fibronectins / Q000494:pharmacology; D006801:Humans; D039001:Integrin alpha Chains; D019012:Integrin beta1; D016023:Integrins / Q000276:immunology*; D008213:Lymphocyte Activation / Q000276:immunology / Q000502:physiology*; D046911:Macromolecular Substances; D016029:Receptors, Very Late Antigen / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology / Q000502:physiology
D015703:Antigens, CD; D017252:CD3 Complex; C063493:CDw49d; D005353:Fibronectins; D039001:Integrin alpha Chains; D019012:Integrin beta1; D016023:Integrins; D046911:Macromolecular Substances; D016029:Receptors, Very Late Antigen