title: Continuous flow synthesis of peptides using a polyacrylamide gel resin (Expansin).
abstract: The continuous flow syntheses of endothelin 1, proendothelin 2, ATP binding site of the CDC2 kinase 3, and fragment 18-30 of an actin 4, have been performed by using a polyacrylamide gel resin Expansin (about 0.6 mmol NH2/g) with the glycolamidic ester handle as labile anchorage. In addition, we report here a method of air oxidation which reduces the formation of side-products related to the formation of intermolecular disulfide bridges.
authors.affiliation: Center of Macromolecular Biochemistry Research, Montpellier, France.
authors.forename: C
authors.initials: C
authors.lastname: Mendre
authors.forename: V
authors.initials: V
authors.lastname: Sarrade
authors.forename: B
authors.initials: B
authors.lastname: Calas
mesh_terms: D000180:Acrylic Resins; D000199:Actins / Q000138:chemical synthesis; D000255:Adenosine Triphosphate / Q000378:metabolism; D000595:Amino Acid Sequence; D001665:Binding Sites; D016203:CDC2 Protein Kinase / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D016232:Endothelins / Q000138:chemical synthesis; D005782:Gels; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000138:chemical synthesis; D010455:Peptides / Q000138:chemical synthesis*; D011498:Protein Precursors / Q000138:chemical synthesis
chemical_list: D000180:Acrylic Resins; D000199:Actins; D016232:Endothelins; D005782:Gels; D010446:Peptide Fragments; D010455:Peptides; D011498:Protein Precursors; C016680:polyacrylamide gels; D000255:Adenosine Triphosphate; D016203:CDC2 Protein Kinase