Application of phosphate-backbone-modified oligonucleotides in the studies on EcoRI endonuclease mechanism of action.
Chemical synthesis of oligodeoxyribonucleotides modified at a preselected internucleotide bond by the replacement of one of the two "nonbridging" oxygens by a sulfur atom or an ethoxy group yields model substrates for studies on DNA-protein interactions. Chromatographic (RP-HPLC) separation of the diastereomers of oligonucleotides containing EcoRI canonical sequence together with the assignment of the substituent orientation in the DNA molecule allowed study of the stereochemical aspects of DNA-EcoRI endonuclease interactions. The DNA segment involved in interactions between EcoRI protein and phosphate groups appeared to be larger than its canonical sequence, ...GAATTC..., and was extended to the nonamer. The modification of certain internucleotide bonds within this nonamer caused significant or complete protection against the nucleolytic action of EcoRI and, in some cases, manifested the diastereoselectivity of the enzyme. On the basis of the results of EcoRI-catalyzed hydrolysis of stereodefined phosphorothioate and phosphotriester substrates, we propose a model to explain this phenomenon at the molecular level.
Koziolkiewicz
M
M
Department of Bioorganic Chemistry, Polish Academy of Sciences, Lodz.
Stec
W J
WJ
D001483:Base Sequence; D015246:Deoxyribonuclease EcoRI / Q000378:metabolism*; D003854:Deoxyribonucleotides / Q000138:chemical synthesis / Q000378:metabolism*; D006868:Hydrolysis; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000138:chemical synthesis / Q000378:metabolism*; D013237:Stereoisomerism; D013379:Substrate Specificity
D003854:Deoxyribonucleotides; D009841:Oligonucleotides; D015246:Deoxyribonuclease EcoRI