Specific MR imaging of human lymphocytes by monoclonal antibody-guided dextran-magnetite particles.
Human lymphocytes were labeled with biotinylated anti-lymphocyte-directed monoclonal antibodies, to which streptavidin and subsequently biotinylated dextran-magnetite particles were coupled. This labeling resulted in a strong and selective negative contrast enhancement of lymphocyte suspensions at 2.0 T, caused predominantly by the specific increase of R2 with a small but significant specific increase of R1. The R1 was found to decrease with increasing field strength. The immunolabeling procedure described here may be used for the selective signal depletion of target cells in MR imaging.
Bulte
J W
JW
Department of Clinical Immunology, University of Groningen, The Netherlands.
Hoekstra
Y
Y
Kamman
R L
RL
Magin
R L
RL
Webb
A G
AG
Briggs
R W
RW
Go
K G
KG
Hulstaert
C E
CE
Miltenyi
S
S
The
T H
TH
D000911:Antibodies, Monoclonal; D001426:Bacterial Proteins; D003287:Contrast Media; D003911:Dextrans; D052203:Ferrosoferric Oxide; D006801:Humans; D066298:In Vitro Techniques; D007501:Iron; D008214:Lymphocytes / Q000473:pathology*; D008279:Magnetic Resonance Imaging / Q000379:methods*; D010087:Oxides; D019809:Streptavidin
D000911:Antibodies, Monoclonal; D001426:Bacterial Proteins; D003287:Contrast Media; D003911:Dextrans; D010087:Oxides; D019809:Streptavidin; D007501:Iron; D052203:Ferrosoferric Oxide