Kedarcidin, a new chromoprotein antitumor antibiotic. II. Isolation, purification and physico-chemical properties.
Kedarcidin, a new chromoprotein antitumor antibiotic, was isolated from the culture broth of a novel actinomycete strain L585-6 (ATCC 53650). The antibiotic was recovered from the culture filtrate by adsorption to QAE ion exchanger and purified by successive application of gel filtration and ion exchange chromatography with Sephadex G-50 and DEAE-Sephadex, respectively. Kedarcidin is an acidic complex (pI 3.65) with an apparent molecular weight of 12,400. The complex consists of a highly unstable, solvent extractable chromophore and a water soluble peptide. The apoprotein is a single chain polypeptide of 114 residues.
Hofstead
S J
SJ
Bristol-Myers Squibb, Pharmaceutical Research Institute, Wallingford, Conn. 06492.
Matson
J A
JA
Malacko
A R
AR
Marquardt
H
H
D000192:Actinomycetales / Q000378:metabolism; D000480:Alkynes / Q000737:chemistry / Q000302:isolation & purification; D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000900:Anti-Bacterial Agents; D000903:Antibiotics, Antineoplastic / Q000737:chemistry* / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D003516:Cycloparaffins / Q000737:chemistry / Q000302:isolation & purification; D053281:Enediynes; D036341:Intercellular Signaling Peptides and Proteins; D007525:Isoelectric Focusing; D008969:Molecular Sequence Data; D009281:Naphthalenes / Q000737:chemistry / Q000302:isolation & purification; D010455:Peptides; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification
D000480:Alkynes; D000596:Amino Acids; D000900:Anti-Bacterial Agents; D000903:Antibiotics, Antineoplastic; D003516:Cycloparaffins; D053281:Enediynes; D036341:Intercellular Signaling Peptides and Proteins; D009281:Naphthalenes; D010455:Peptides; D011506:Proteins; C069636:kedarcidin peptide; C080542:kedarcidin chromophore