Serotonin uptake by astrocytes in situ.
Co-localization of glial fibrillary acidic protein (GFAP) and radioactivity was examined after intraventricular injection of [3H]5-HT in adult rat brains. Radioactivity localized over GFAP-positive astrocytes was seen, especially when image-enhancing techniques were applied to the data. Also slices prepared from astrogliotic hippocampi of rats pretreated with kainic acid showed a twofold increased uptake of [3H]5-HT compared to control slices. This indicates that the uptake of [3H]5-HT seen in primary astrocyte cultures also occurs for astrocytes in situ. Also, as with astrocyte cultures, only some of the GFAP(+) astrocytes in situ showed localization of radioactivity, supporting the concept of intraregional heterogeneity of astrocyte functions.
Anderson
E J
EJ
Division of Neurosurgery, Albany Medical College, New York 12204.
McFarland
D
D
Kimelberg
H K
HK
D000818:Animals; D001253:Astrocytes / Q000378:metabolism*; D001923:Brain Chemistry / Q000502:physiology; D005911:Gliosis / Q000378:metabolism; D006624:Hippocampus / Q000378:metabolism; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D007276:Injections, Intraventricular; D007608:Kainic Acid / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012701:Serotonin / Q000378:metabolism*
D012701:Serotonin; D007608:Kainic Acid