Studies of the low dose 'hook' effect in a competitive homogeneous immunoassay.
The interactions of two monoclonal antibodies with human growth hormone (hGH) have been investigated. The individual antibodies showed normal behavior in a competitive binding assay, but mixtures of the antibodies demonstrated a 'hook' attributable to cooperative interactions. Cooperativity was observed in titrations which preceded the competitive binding assay. Size exclusion chromatographic data suggest that the cooperativity is explained by the formation of higher molecular weight complexes (up to 700 kDa). The major complex is probably linear, consisting of three antibody molecules. Circular and linear complexes with four antibody molecules (octameric complexes) are also possible. Theoretical models also support the formation of cyclic complexes in a competitive binding assay.
Fernando
S A
SA
Department of Chemistry, University of Kansas, Lawrence 66045.
Sportsman
J R
JR
Wilson
G S
GS
D000494:Allosteric Regulation; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000937:Antigen-Antibody Reactions; D001667:Binding, Competitive; D004306:Dose-Response Relationship, Immunologic; D000939:Epitopes; D013006:Growth Hormone / Q000032:analysis / Q000276:immunology*; D006801:Humans; D007118:Immunoassay / Q000379:methods; D066298:In Vitro Techniques; D046911:Macromolecular Substances
D000911:Antibodies, Monoclonal; D000939:Epitopes; D046911:Macromolecular Substances; D013006:Growth Hormone