Separation of reduced and oxidized glutathione in a pharmaceutical preparation by ion-interaction reversed-phase HPLC.
A new method is presented for the simultaneous determination of reduced and oxidized glutathione by ion-interaction reversed-phase HPLC with octylamine orthophosphate as the interaction reagent, 5-microns Spherisorb ODS-2 as the stationary phase and UV detection at 230 nm. Lower limits of detection of 2.50 micrograms ml-1 have been achieved for both the oxidized and the reduced forms. The method has been applied in the analysis of a commercial pharmaceutical preparation.
Gennaro
M C
MC
Dipartimento di Chimica Analitica, Università di Torino, Italy.
Abrigo
C
C
D000975:Antioxidants / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005978:Glutathione / Q000032:analysis*; D010084:Oxidation-Reduction
D000975:Antioxidants; D005978:Glutathione