The structure of a glycerol teichoic acid-like O-specific polysaccharide of Hafnia alvei 1205.
The O-specific polysaccharide of Hafnia alvei 1205 contained D-glucose, D-galactose, 2-acetamido-2-deoxy-D-glucose, 4-acetamido-4,6-dideoxy-D-glucose (Qui4NAc), glycerol, phosphate, and O-acetyl groups. On the basis of 1D and 2D shift-correlated homonuclear and 13C-1H heteronuclear NMR spectroscopy, methylation analysis, Smith degradation, and dephosphorylation with hydrofluoric acid, it was concluded that the O-antigen was a partially O-acetylated teichoic acid-like polysaccharide having the following structure: [formula: see text]
Katzenellenbogen
E
E
L. Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław.
Romanowska
E
E
Kocharova
N A
NA
Knirel
Y A
YA
Shashkov
A S
AS
Kochetkov
N K
NK
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D004755:Enterobacteriaceae / Q000737:chemistry*; D005990:Glycerol / Q000032:analysis*; D007202:Indicators and Reagents; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008745:Methylation; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry / Q000302:isolation & purification; D009904:Optical Rotation; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000302:isolation & purification; D013682:Teichoic Acids / Q000737:chemistry*
D007202:Indicators and Reagents; D009844:Oligosaccharides; D011135:Polysaccharides, Bacterial; D013682:Teichoic Acids; D005990:Glycerol