title: Effects of macrophage colony-stimulating factor on the activities of murine monocytes and peritoneal macrophages in vivo.
abstract: The ability of peripheral blood monocytes, granulocytes and resident peritoneal macrophages to generate hydrogen peroxide in response to concanavalin A was enhanced by a single intravenous injection of macrophage colony-stimulating factor (M-CSF) of recombinant human type in AKR mice. In response to phorbol myristate acetate, only granulocytes and resident peritoneal macrophages showed the enhanced ability. Phagocytosis by those cells was not stimulated by M-CSF. Surface marker analysis showed an increased expression of F4/80 and Mac1 on monocytes, Mac1 expression on granulocytes and LFA-1 expression on resident peritoneal macrophages. Ia antigen on resident peritoneal macrophages was suppressed by M-CSF. M-CSF can induce monocytes, granulocytes and resident peritoneal macrophages to generate hydrogen peroxide and enhances their maturation in vivo.
authors.affiliation: Department of Microbiology, National Defense Medical College, Saitama, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Katsura
authors.forename: S
authors.initials: S
authors.lastname: Tsuru
authors.forename: M
authors.initials: M
authors.lastname: Noritake
authors.forename: N
authors.initials: N
authors.lastname: Shinomiya
authors.forename: S
authors.initials: S
authors.lastname: Kayashima
authors.forename: K
authors.initials: K
authors.lastname: Motoyoshi
authors.forename: M
authors.initials: M
authors.lastname: Rokutanda
mesh_terms: D000818:Animals; D000954:Antigens, Surface / Q000276:immunology; D005434:Flow Cytometry; D006098:Granulocytes / Q000276:immunology; D016173:Macrophage Colony-Stimulating Factor / Q000276:immunology*; D008264:Macrophages / Q000276:immunology*; D008297:Male; D051379:Mice; D008806:Mice, Inbred AKR; D009000:Monocytes / Q000276:immunology*; D010529:Peritoneal Cavity; D010587:Phagocytosis / Q000276:immunology; D011994:Recombinant Proteins / Q000276:immunology
chemical_list: D000954:Antigens, Surface; D011994:Recombinant Proteins; D016173:Macrophage Colony-Stimulating Factor