Inhibition of DNA polymerase activity by thymine hydrates.
Ultraviolet irradiation of DNA results in various pyrimidine modifications. We have demonstrated formation of both cis-thymine hydrate and trans-thymine hydrate (6-hydroxy-5,6-dihydrothymine) in UV-irradiated poly(dA-dT):poly(dA-dT). Both are released from DNA as free bases by bacterial and human glycosylases. Thymine hydrates are stable in DNA and can be detected in control, unirradiated substrates. We examined the effects of thymine hydrates in UV-irradiated substrate poly(dA-dT):poly(dA-dT) on E. coli DNA polymerase I activity. Enzymic incorporation of labeled thymidine-5'-monophosphate significantly decreased with increasing UV dose. Reversal of DNA thymine hydrates to thymines by mild heating of the substrate prior to enzymic reaction resulted in partial recovery of nucleotide incorporation. Cyclobutane thymine dimers are formed between non-adjacent thymines in UV-irradiated poly(dA-dT):poly(dA-dT). These are responsible for the incomplete recovery of DNA polymerase activity following heating due to their heat stability. Analyses of the irradiated and hydrolyzed substrate also demonstrated formation of minor yields of photoproducts formed by covalent linkage of adjacent thymines and adenines by UV-irradiation. Therefore, the thymine hydrates formed in UV-irradiated DNA partially inhibit polymerase activity during DNA synthesis and thus could be potentially lethal if unrepaired.
Ganguly
T
T
Department of Pathology, Temple University School of Medicine, Philadelphia, PA 19140.
Duker
N J
NJ
D002851:Chromatography, High Pressure Liquid; D002854:Chromatography, Paper; D004247:DNA / Q000096:biosynthesis / Q000737:chemistry / Q000187:drug effects* / Q000528:radiation effects; D004256:DNA Polymerase I / Q000037:antagonists & inhibitors*; D004795:Enzyme Stability; D004926:Escherichia coli / Q000201:enzymology; D006358:Hot Temperature; D011067:Poly dA-dT / Q000737:chemistry / Q000528:radiation effects; D013938:Thymidine Monophosphate / Q000378:metabolism; D013941:Thymine / Q000031:analogs & derivatives* / Q000494:pharmacology; D014466:Ultraviolet Rays
C066110:6-hydroxy-5,6-dihydrothymine; D011067:Poly dA-dT; D013938:Thymidine Monophosphate; D004247:DNA; D004256:DNA Polymerase I; D013941:Thymine