Decrease in rat brain calcitonin binding sites and adenylyl-cyclase activity during ageing.
In order to investigate the effects of ageing on the cerebral receptors for calcitonin (CT), we used an in vitro autoradiographic method to study the distribution of the binding sites for eel CT (eCT) in young and old rat brain. The inhibitory action of eCT on adenylyl-cyclase (AC) activity upon isolated brain cell membranes was also evaluated. The results show area-specific reduction of binding particularly in the hypothalamus and pons medulla of the old rat. The inhibitory action of eCT on AC activity was significantly reduced in the same areas, whereas in the striatum and mesencephalon no changes were observed. The parallel decrease of binding of eCT and of the inhibitory action of eCT on AC in ageing may represent a functional decline of neuronal activities during ageing.
Villa
I
I
Department of Pharmacology, Chemotherapy and Medical Toxicology, University of Milan, Italy.
Guidobono
F
F
Netti
C
C
Pecile
A
A
Giovanazzi
S
S
Nicosia
S
S
D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D000262:Adenylyl Cyclases / Q000378:metabolism*; D000375:Aging / Q000378:metabolism*; D000818:Animals; D001345:Autoradiography; D001921:Brain / Q000254:growth & development / Q000378:metabolism*; D002116:Calcitonin / Q000378:metabolism* / Q000494:pharmacology; D002250:Carbon Radioisotopes; D000242:Cyclic AMP / Q000378:metabolism; D007457:Iodine Radioisotopes; D007700:Kinetics; D008297:Male; D009928:Organ Specificity; D051381:Rats; D017208:Rats, Wistar; D018003:Receptors, Calcitonin; D011956:Receptors, Cell Surface / Q000378:metabolism*; D014316:Tritium
D002250:Carbon Radioisotopes; D007457:Iodine Radioisotopes; D018003:Receptors, Calcitonin; D011956:Receptors, Cell Surface; D014316:Tritium; D002116:Calcitonin; D000242:Cyclic AMP; D000262:Adenylyl Cyclases; D015056:1-Methyl-3-isobutylxanthine