Influence of long-term hypoxia on the energy metabolism of the haemoglobin-containing bivalve Scapharca inaequivalvis: critical O2 levels for metabolic depression.
The oxygen consumption rate of Scapharca inaequivalvis measured under normoxic conditions over 48 h showed a significant daily cycle with lowest values occurring shortly after the dark period; all hypoxia exposure experiments were carried out during the declining part of the cycle. Animals were exposed to a constant level of hypoxia for a 12-h period in a series of 14 experiments, each at a different oxygen tension. The oxygen consumption was measured continuously, and the extent of accumulation of end-products (succinate and propionate), and the inhibitory effect of adenosine triphosphate on phosphofructokinase were determined at the end of exposures. All three parameters (oxygen consumption, end-product accumulation, phosphofructokinase inhibition) showed a remarkable correlation with major changes occurring between 2.5 and 1.5 ppm (7 and 4 kPa) O2. The oxygen consumption rates showed a drop to 6% of the normoxic rate, but a consistent low consumption remained below 2 ppm (5.5 kPa) which partly recovered over the 12-h exposure period by about three-fold. Succinate and propionate accumulated progressively between 2.5 and 1.5 ppm (7 and 4 kPa); at [O2] less than 1.5 ppm (4 kPa) the concentration did not increase further, indicating that anaerobic metabolism had reached a maximum. Over the same range, phosphofructokinase showed an increased sensitivity for adenosine triphosphate, the lower inhibitor concentration at 50% Vmax value pointing to depression of glycolytic rate. Despite the activation of anaerobic metabolism and the evident depression of aerobic metabolism, simple calculation demonstrates that Scapharca inaequivalvis relies mainly on aerobic metabolism even during severe hypoxia.(ABSTRACT TRUNCATED AT 250 WORDS)
van den Thillart
G
G
University of Leiden, Department of Biology, Gorlaeus laboratoria, The Netherlands.
van Lieshout
G
G
Storey
K
K
Cortesi
P
P
de Zwaan
A
A
D000255:Adenosine Triphosphate / Q000378:metabolism / Q000494:pharmacology; D000693:Anaerobiosis; D000818:Animals; D049872:Bivalvia / Q000378:metabolism*; D004734:Energy Metabolism; D006454:Hemoglobins / Q000378:metabolism; D000860:Hypoxia / Q000378:metabolism; D010101:Oxygen Consumption; D010732:Phosphofructokinase-1 / Q000037:antagonists & inhibitors / Q000378:metabolism
D006454:Hemoglobins; D000255:Adenosine Triphosphate; D010732:Phosphofructokinase-1