title: Distribution of putative odour receptor proteins in olfactory epithelium.
abstract: To investigate immunohistochemically the spatial localization of putative odour receptor proteins, we synthesized a polypeptide which corresponds to a region of a putative odour receptor protein, I3, and raised an antibody to the peptide. In rat olfactory epithelium, the antibody specifically recognized cilia of a small subset of olfactory receptor neurons, suggesting that the odour receptor protein is localized selectively in the cilia. Olfactory receptor neurons having immunoreactive cilia were distributed sparsely throughout the epithelium. This suggests that receptor neurons expressing a similar odour receptor protein are probably distributed similarly in the epithelium.
authors.affiliation: Department of Neuroscience, Osaka Bioscience Institute, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Koshimoto
authors.forename: K
authors.initials: K
authors.lastname: Katoh
authors.forename: Y
authors.initials: Y
authors.lastname: Yoshihara
authors.forename: K
authors.initials: K
authors.lastname: Mori
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies; D002352:Carrier Proteins / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D004847:Epithelial Cells; D007150:Immunohistochemistry; D008297:Male; D008969:Molecular Sequence Data; D009831:Olfactory Mucosa / Q000166:cytology*; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology; D011487:Protein Conformation; D011817:Rabbits / Q000276:immunology; D051381:Rats; D018035:Receptors, Odorant; D011984:Sensory Receptor Cells / Q000648:ultrastructure*
chemical_list: D000906:Antibodies; D002352:Carrier Proteins; D010455:Peptides; D018035:Receptors, Odorant; C045648:odorant-binding protein