title: Sequences encoding the protein and RNA components of ribonuclease P from Streptomyces bikiniensis var. zorbonensis.
abstract: The genes encoding the RNA (rnpB) and protein (rnpA) subunits of ribonuclease P (RNase P) of Streptomyces bikiniensis var. zorbonensis have been cloned by complementing the temperature-sensitive growth phenotype of Escherichia coli strains that carry mutations in these genes. The rnpB sequence of S. bikiniensis includes new covariations that lead to refinement of the previous secondary structure models for RNase P RNAs. The deduced amino acid sequence of S. bikiniensis RNase P is conserved with that of other known RNase P proteins only to a limited extent. Immediately upstream from rnpA is an open reading frame that codes for the highly conserved ribosomal protein, L34. This same gene arrangement occurs in all bacteria studied to date.
authors.affiliation: Department of Biochemistry, University of Missouri-Columbia 65212.
authors.forename: D P
authors.initials: DP
authors.lastname: Morse
authors.forename: F J
authors.initials: FJ
authors.lastname: Schmidt
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D004269:DNA, Bacterial; D004722:Endoribonucleases / Q000235:genetics* / Q000302:isolation & purification; D029968:Escherichia coli Proteins; D014644:Genetic Variation; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012329:RNA, Bacterial / Q000737:chemistry / Q000235:genetics*; D016337:RNA, Catalytic / Q000235:genetics* / Q000302:isolation & purification; D043262:Ribonuclease P; D016415:Sequence Alignment; D013302:Streptomyces / Q000201:enzymology*
chemical_list: D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; D012329:RNA, Bacterial; D016337:RNA, Catalytic; D004722:Endoribonucleases; D043262:Ribonuclease P; C470865:ribonuclease P, E coli