The pp60c-src in retinal pigment epithelium and its modulation by vasoactive intestinal peptide.
The pp60c-src is present at high level in differentiated retinal pigment epithelium (RPE) in culture. Immunofluorescence microscopy using GD11 (anti-pp60c-src) shows intense staining in the plasma membrane, especially at the cell-cell junctions, and diffuse staining in the cytoplasma. Western blot analysis shows that the majority of the GD11-reactive molecules is localized in the membrane. The pp60c-src does not translocate between membrane and cytoplasma when the RPE was reacted with vasoactive intestinal peptide (VIP), which is previously shown to stimulate phosphorylation of the pp60c-src in the membranes (Biochem. Biophys. Res. Commun. 174, 452-8, 1991). Here, VIP modulation is shown to alter the reactivity of pp60c-src with a monoclonal anti-phosphotyrosine antibody.
Koh
S W
SW
Department of Ophthalmology, University of Maryland, Baltimore 21201.
D000818:Animals; D015153:Blotting, Western; D002478:Cells, Cultured; D002642:Chick Embryo; D008856:Microscopy, Fluorescence; D010857:Pigment Epithelium of Eye / Q000737:chemistry / Q000187:drug effects*; D016392:Proto-Oncogene Proteins pp60(c-src) / Q000187:drug effects*; D014660:Vasoactive Intestinal Peptide / Q000494:pharmacology*
D014660:Vasoactive Intestinal Peptide; D016392:Proto-Oncogene Proteins pp60(c-src)