title: Immunohistochemical localization of basal lamina components in the developing rat epiphyseal cartilage canals.
abstract: The purpose of this study was to investigate the relationship between the appearance of basal lamina components (Type IV collagen and laminin) and the development of cartilage canals. In the distal femoral epiphyses from developing rats, the distribution of basal lamina components in the cartilage canal was examined immunohistochemically. The formation of cartilage canals from the perichondrium was first observed on the fifth day after birth. By Day 8, a few cartilage canals penetrated the epiphyseal cartilage and considerably increased in size and length. By light microscopic immunohistochemistry, reticular structures stained with anti-Type IV collagen and antilaminin antibodies were observed in the cartilage canals. In the early development of cartilage canals, however, immunostaining by anti-Type IV collagen antibodies was weaker than that by antilaminin antibodies. In eight-day-old rats, the laminin-positive reticular structures were more densely colored and more widely distributed in the canal than the Type IV collagen-positive ones. Type IV collagen was found around the endothelial cells of the developing capillaries by electron microscopic immunohistochemistry. Laminin was observed in the cytoplasm of the mesenchymal fibroblastic cells and their pericellular matrix as well as in the capillary basal lamina. These immunohistochemical electron microscopic observations can explain the differences that are observed in Type IV collagen and laminin immunostaining patterns as cartilage canals develop. Laminin synthesized by the mesenchymal fibroblastic cells may promote the migration and the outgrowth of endothelial cells in the formation of cartilage canals.
authors.affiliation: Department of Orthopaedic Surgery, Kumamoto University Medical School, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Kai
authors.forename: K
authors.initials: K
authors.lastname: Takagi
authors.forename: K
authors.initials: K
authors.lastname: Iyama
authors.forename: M
authors.initials: M
authors.lastname: Kitaoka
authors.forename: H
authors.initials: H
authors.lastname: Yoshioka
authors.forename: H
authors.initials: H
authors.lastname: Mizuta
authors.forename: G
authors.initials: G
authors.lastname: Usuku
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D000906:Antibodies / Q000302:isolation & purification; D001485:Basement Membrane / Q000378:metabolism*; D003094:Collagen / Q000032:analysis; D004730:Endothelium, Vascular / Q000648:ultrastructure; D006132:Growth Plate / Q000098:blood supply* / Q000378:metabolism; D007150:Immunohistochemistry; D007797:Laminin / Q000032:analysis; D009389:Neovascularization, Pathologic; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D000906:Antibodies; D007797:Laminin; D003094:Collagen