Effects of postpubertal treatment with progesterone on protein expression in the vagina and uterus of mice exposed neonatally to diethylstilbestrol.
Postpubertal progesterone injections slightly inhibited the proliferation and cornification of the vaginal epithelium induced by neonatal injections of diethylstilbestrol (DES). In vaginae of neonatally DES-exposed mice (DES mice), 9 protein expressions (PEX) appeared newly; 13 PEX decreased; 5 PEX increased compared to those in the controls. In vaginae of DES mice, PEX were altered by postpubertal injections of progesterone (DES-P); 3 PEX disappeared; 11 PEX were reversed to those in the controls. Progesterone injections impaired the proliferation of the vaginal epithelium, reversing 11 PEX compared to those in DES mice. In uteri of DES-P mice, 3 PEX increased, 2 PEX decreased, 2 PEX appeared and 1 PEX disappeared compared to those in DES mice. In conclusion, it was shown that postpubertal injections of progesterone alter the protein expression in the vagina and uterus of DES mice.
Takamatsu
Y
Y
Graduate School of Integrated Science, Yokohama City University, Japan.
Iguchi
T
T
Takasugi
N
N
D000818:Animals; D000831:Animals, Newborn; D002455:Cell Division / Q000187:drug effects; D004054:Diethylstilbestrol / Q000008:administration & dosage / Q000037:antagonists & inhibitors*; D015180:Electrophoresis, Gel, Two-Dimensional; D004848:Epithelium / Q000187:drug effects; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D051379:Mice; D010052:Ovariectomy; D011374:Progesterone / Q000494:pharmacology*; D014176:Protein Biosynthesis; D012741:Sexual Maturation; D014599:Uterus / Q000187:drug effects* / Q000378:metabolism; D014621:Vagina / Q000187:drug effects* / Q000378:metabolism
D011374:Progesterone; D004054:Diethylstilbestrol