Immunohistochemical organization of the ventral lateral geniculate nucleus in the ground squirrel.
The ventral lateral geniculate nucleus (vLGN) of the thirteen-lined ground squirrel (Citellus tridecemlineatus) is a highly differentiated nucleus that is divisible into five major subdivisions on the basis of retinal projections and cytoarchitecture. To pursue the likelihood that these subdivisions (the dorsal cap, intergeniculate leaflet, external magnocellular lamina, internal magnocellular lamina, and parvicellular segment) correlate with the functional diversity of this complex, the present study examined the neurochemical composition of the vLGN with regard to substances that have previously proved useful in distinguishing functionally distinct subregions within nuclei (i.e., neuropeptide Y (NPY), substance P (SP), leucine and methionine enkephalins, gamma-aminobutyric acid (GABA), cytochrome oxidase (CO), acetylcholinesterase (AChE), and NADPH-diaphorase). The results showed a clear differential neurochemical distribution within the nucleus. Neuropeptide Y immunoreactive perikarya were found predominantly in the intergeniculate leaflet and external magnocellular lamina, with only a few present in the internal magnocellular lamina and dorsal cap, and none observed in the parvicellular segment. NPY+ fibers, however, were present in all divisions except the parvicellular segment. The highest concentration of SP immunoreactive cells was observed in the internal magnocellular lamina, and substantial numbers also were scattered in the external magnocellular lamina and parvicellular segment. SP+ fibers were seen predominantly in the intergeniculate leaflet and the magnocellular laminae. The heaviest concentration of enkephalinergic fibers occurred in the internal magnocellular lamina and dorsal cap, but fibers were also observed in the external magnocellular lamina and intergeniculate leaflet. GABA reactivity was widespread throughout the vLGN, with the dorsal cap and external magnocellular lamina most heavily labeled, followed by the intergeniculate leaflet and the internal magnocellular lamina. Cytochrome oxidase, AChE, and NADPH-diaphorase histochemistry revealed rich reactivity within the dorsal cap, and external and internal magnocellular laminae and paler reactivity in the intergeniculate leaflet and parvicellular segment. The external magnocellular lamina was more reactive for CO and NADPH-diaphorase than AChE, while the internal magnocellular lamina showed the opposite pattern of reactivity. In addition, NADPH-diaphorase reactive cells were present in caudal intergeniculate leaflet and lateral external magnocellular lamina. These local differences in the neurochemical character of the vLGN support its parcellation into multiple subdivisions. Taken in conjunction with the differences in cytoarchitecture and retinal projections, these results suggest substantial functional diversity within the ventral lateral geniculate complex.
Agarwala
S
S
Department of Psychology, State University of New York, Stony Brook 11794.
May
J G
JG
Moore
J K
JK
Petry
H M
HM
D000110:Acetylcholinesterase / Q000378:metabolism; D000818:Animals; D015415:Biomarkers; D003576:Electron Transport Complex IV / Q000378:metabolism; D004745:Enkephalins / Q000378:metabolism; D005829:Geniculate Bodies / Q000033:anatomy & histology* / Q000166:cytology / Q000502:physiology; D007150:Immunohistochemistry; D009252:NADPH Dehydrogenase / Q000378:metabolism; D009478:Neuropeptide Y / Q000378:metabolism; D012160:Retina / Q000502:physiology; D012589:Sciuridae / Q000033:anatomy & histology* / Q000502:physiology; D013194:Staining and Labeling; D013373:Substance P / Q000378:metabolism; D014795:Visual Pathways / Q000502:physiology; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
D015415:Biomarkers; D004745:Enkephalins; D009478:Neuropeptide Y; D013373:Substance P; D005680:gamma-Aminobutyric Acid; D009252:NADPH Dehydrogenase; D003576:Electron Transport Complex IV; D000110:Acetylcholinesterase