Simple assays of retinoid activity as potential screens for compounds that may be useful in treatment of psoriasis.
Human epidermal cell cultures were used to study the effects of retinoids on keratinocyte differentiation. Keratin profiles were studied by quantitative gel electrophoresis of culture extracts, whereas the extent of envelope formation was assessed in an enzyme-linked immunosorbent assay (ELISA) using an antibody that specifically recognizes keratinocyte envelopes. Exposure of cultures to a variety of different retinoids produced both dose-dependent decreases in keratin 16 with consequent increases in the keratin 14: keratin 16 ratio, and a decrease in envelope formation. The order of activity in both assays was similar: arotinoid ethyl ester (Ro 13-6298) greater than or equal to arotinoid acid (Ro 13-7410) much greater than all trans retinoic acid (Ro 1-5488) greater than acitretin (Ro 10-1670) greater than or equal to etretinate (Ro 10-9359), the only difference being that acitretin was slightly more active than etretinate in the keratin assay whereas these retinoids were equi-active in the envelope assay. Analysis of the lesional keratins of psoriasis patients showed that etretinate caused a reduction in keratin 16 and an increase in the keratin 14:keratin 16 ratio, although the magnitude of these changes and their correlation with clinical improvement was variable. As the in vitro assays reported here are simple and quick, they allow rapid screening of compounds for retinoid-like activity.
West
M R
MR
Department of Cellular Science, Glaxo Group Research, Middlesex, U.K.
Page
J M
JM
Turner
D M
DM
Wood
E J
EJ
Holland
D B
DB
Cunliffe
W J
WJ
Rupniak
H T
HT
D016475:3T3 Cells; D000818:Animals; D001706:Biopsy; D002454:Cell Differentiation / Q000187:drug effects; D002462:Cell Membrane / Q000187:drug effects / Q000502:physiology; D005050:Etretinate / Q000627:therapeutic use; D006128:Growth / Q000187:drug effects; D006801:Humans; D015603:Keratinocytes / Q000166:cytology; D007633:Keratins / Q000032:analysis; D051379:Mice; D011565:Psoriasis / Q000188:drug therapy* / Q000378:metabolism; D012176:Retinoids / Q000627:therapeutic use*; D012867:Skin / Q000166:cytology / Q000473:pathology
D012176:Retinoids; D005050:Etretinate; D007633:Keratins