Human anti-F(ab')2 antibodies show preferential reactivity for F(ab')2 molecules bearing lambda light chains.
In order to evaluate binding specificities of anti-F(ab')2 antibodies from patients with systemic lupus erythematosus (SLE) and from normal healthy controls, F(ab')2 fragments were prepared from 24 IgG myelomas with defined isoelectric points, DNA-associated idiotypes, and kappa/lambda light chain types. Using ELISA and hemagglutination assays, anti-F(ab')2 antibodies from 12 healthy controls and 29 SLE patients were observed to exhibit preferential binding (lambda > kappa) to myeloma F(ab')2 fragments composed of lambda light chains (P < 0.0001). No correlation of anti-F(ab')2 binding and presence of cationic, neutral, or anionic isoelectric points or for DNA-associated idiotypes on monoclonal F(ab')2 was detected. Anti-F(ab')2 antibodies, often elevated in SLE during remission, show preferential specificity for F(ab')2 fragments bearing lambda light chains.
Yancey
W B
WB
Department of Medicine, University of Florida College of Medicine, Gainesville.
Silvestris
F
F
Conlon
M
M
Rodriguez
M
M
Malone
C
C
Williams
R C
RC
D000328:Adult; D000888:Antibodies, Anti-Idiotypic / Q000378:metabolism*; D000974:Antibodies, Antinuclear / Q000276:immunology; D000918:Antibody Specificity; D004588:Electrophoresis, Cellulose Acetate; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006386:Hemagglutination Tests; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000378:metabolism*; D007145:Immunoglobulin kappa-Chains / Q000378:metabolism; D007146:Immunoglobulin lambda-Chains / Q000378:metabolism*; D007526:Isoelectric Point; D008180:Lupus Erythematosus, Systemic / Q000276:immunology*; D009101:Multiple Myeloma / Q000276:immunology
D000888:Antibodies, Anti-Idiotypic; D000974:Antibodies, Antinuclear; D007140:Immunoglobulin Fab Fragments; D007145:Immunoglobulin kappa-Chains; D007146:Immunoglobulin lambda-Chains