Inhibition of intraocular fibrin formation with annexin V.
Annexin V is a member of the calcium- and phospholipid-binding proteins, known to have an antithrombotic effect. For the first time, we have tested its ability to prevent intraocular postoperative fibrin formation in a standardised rabbit model and compared its effect with that of heparin. Annexin V, 20 micrograms and 60 micrograms, injected in the anterior chamber post-operatively, significantly reduced the area of the fibrin clot and its time to clearing. Annexin V appeared to be as efficient as heparin. It probably acts by preventing phospholipids from playing their role in the coagulation cascade which leads to fibrin formation. Furthermore, annexin V has an anti-inflammatory effect by protecting phospholipids from phospholipase A2 activity. Therefore, annexin V might be considered as a new therapeutic agent acting both on fibrin formation and inflammatory processes.
Chollet
P
P
Laboratory of Ophthalmology, Hôpital de Rangueil, Toulouse, France.
Malecaze
F
F
Hullin
F
F
Raynal
P
P
Arne
J L
JL
Pagot
V
V
Ragab-Thomas
J
J
Chap
H
H
D000818:Animals; D017304:Annexin A5 / Q000494:pharmacology*; D000867:Anterior Chamber / Q000378:metabolism; D005123:Eye / Q000187:drug effects; D005337:Fibrin / Q000096:biosynthesis*; D006493:Heparin / Q000494:pharmacology; D008954:Models, Biological; D013508:Ophthalmologic Surgical Procedures; D011183:Postoperative Complications / Q000378:metabolism / Q000517:prevention & control*; D011817:Rabbits
D017304:Annexin A5; D005337:Fibrin; D006493:Heparin