A and B antigenicity of human spermatozoa and buccal epithelial cells.
The source of A and B antigens on the surface of erythrocytes, spermatozoa and buccal epithelial cells was studied using two kinds of monoclonal antibodies of differing specificity. The first, anti-Ar, reacts with the erythrocyte A antigen but not with the soluble A antigen found in secretions such as saliva. Similarly, anti-Br reacts with the erythrocyte B antigen but not with the soluble B antigen present in secretions. The second type of monoclonal antibody, anti-Ar+s, reacts with both the erythrocyte A antigen and with the soluble A blood group substance. Similarly, the anti-Br+s reacts with both the erythrocyte B antigen and with the soluble B blood group substance. The spermatozoal A antigen reacts only with the anti-Ar+s monoclonal antibody whereas buccal epithelial A antigen reacts with both anti-Ar and with anti-Ar+s. Spermatozoal B antigen reacts only with anti-Br+s whereas buccal epithelial B antigen reacts with both anti-Br and anti-Br+s. Buccal cell a and B antigens appear to be integral to the membrane or at least tightly bound whereas the spermatozoal A and B antigens are adsorbed from seminal plasma and loosely bound.
Chen
J
J
John Jay College of Criminal Justice, City University of New York, New York 10019.
Kobilinsky
L
L
Ishizu
H
H
Yamamoto
Y
Y
D000017:ABO Blood-Group System / Q000032:analysis*; D000327:Adsorption; D000911:Antibodies, Monoclonal / Q000276:immunology; D000954:Antigens, Surface / Q000276:immunology; D004848:Epithelium / Q000276:immunology; D000939:Epitopes / Q000276:immunology; D004910:Erythrocyte Membrane / Q000276:immunology; D005455:Fluorescent Antibody Technique; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000276:immunology; D009061:Mouth Mucosa / Q000276:immunology*; D009928:Organ Specificity; D012471:Salivary Proteins and Peptides / Q000276:immunology; D012661:Semen / Q000276:immunology; D012995:Solubility; D013094:Spermatozoa / Q000276:immunology*
D000017:ABO Blood-Group System; D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D000939:Epitopes; D008565:Membrane Proteins; D012471:Salivary Proteins and Peptides