Fetal red cell staining: method evaluation.
Acid elution methods have been extensively used for the identification of fetal red cells in pregnant women with fetomaternal hemorrhage. A number of methods have been described: Kleihauer-Betke, Boehringer Mannheim, Fetaldex, Nierhaus-Betke modification and Sanguansermsri amido black B. A comparative evaluation of these methods was performed in this study. The technique of Sanguansermsri using amido black B was found to be more advantageous than the others in terms of simplicity, accuracy, precision and time taken.
Apibal
S
S
Hematology Unit, Children Hospital, Bangkok, Thailand.
Hongyantarachi
A
A
Chiewsilp
P
P
Fucharoen
S
S
D004906:Erythrocyte Count; D005069:Evaluation Studies as Topic; D005260:Female; D005312:Fetal Blood / Q000166:cytology*; D005331:Fetomaternal Transfusion / Q000097:blood* / Q000453:epidemiology; D006801:Humans; D008297:Male; D011247:Pregnancy; D015203:Reproducibility of Results