title: Inhibition by mepacrine and amylase secretion from intact and permeabilized rat pancreatic acini.
abstract: In intact rat pancreatic acini, the phospholipase A2 inhibitor mepacrine did not affect basal amylase release but dose-dependently inhibited the carbachol (IC50 65 microM) and CCK-8 (IC50 210 microM)-stimulated amylase release. In permeabilized acini, mepacrine shifted the dose-response curve for calcium to the right by a factor 2 and inhibited the release of amylase stimulated by GTPrS. From these results we conclude that carbachol, CCK-8 and GTPrS probably activate a phospholipase A2 closely coupled to exocytosis.
authors.affiliation: Department of General and Human Biochemistry, Free University of Brussels, Belgium.
authors.forename: K
authors.initials: K
authors.lastname: Grosfils
authors.forename: F
authors.initials: F
authors.lastname: Gomez
authors.forename: J P
authors.initials: JP
authors.lastname: Dehaye
mesh_terms: D000681:Amylases / Q000378:metabolism*; D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002217:Carbachol / Q000494:pharmacology; D002463:Cell Membrane Permeability; D004305:Dose-Response Relationship, Drug; D004533:Egtazic Acid / Q000494:pharmacology; D066298:In Vitro Techniques; D007700:Kinetics; D008297:Male; D010179:Pancreas / Q000166:cytology / Q000187:drug effects / Q000201:enzymology*; D011796:Quinacrine / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D012844:Sincalide / Q000494:pharmacology
chemical_list: D004533:Egtazic Acid; D002217:Carbachol; D000681:Amylases; D011796:Quinacrine; D012844:Sincalide; D002118:Calcium