Lipopolysaccharide induces prostaglandin H synthase-2 in alveolar macrophages.
Prostaglandin H synthase is a key enzyme in the formation of prostaglandins and thromboxane from arachidonic acid. The recent cloning of a second prostaglandin H synthase gene, prostaglandin H synthase-2, which is distinct from the classic prostaglandin H synthase-1 gene, may dramatically alter our concept of how cells regulate prostanoid formation. We have recently shown that the enhanced production of prostanoids by lipopolysaccharide-primed alveolar macrophages involves the induction of a novel prostaglandin H synthase (J. Biol. Chem., (1992), 267, 14547-14550). We report here that the novel PGH synthase induced by lipopolysaccharide in alveolar macrophages is prostaglandin H synthase-2.
O'Sullivan
M G
MG
Department of Medicine, Wake Forest University Medical Center Winston-Salem, NC 27157-1042.
Huggins
E M
EM
Meade
E A
EA
DeWitt
D L
DL
McCall
C E
CE
D000818:Animals; D015153:Blotting, Western; D004790:Enzyme Induction; D000939:Epitopes / Q000276:immunology; D007163:Immunosorbent Techniques; D008070:Lipopolysaccharides; D016676:Macrophages, Alveolar / Q000201:enzymology*; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D011451:Prostaglandin-Endoperoxide Synthases / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D012333:RNA, Messenger / Q000096:biosynthesis; D011817:Rabbits; D014162:Transfection
D000939:Epitopes; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D011451:Prostaglandin-Endoperoxide Synthases