Autoantibodies in HIV-infected hemophilia patients against different epitopes on CD4+ lymphocytes and recombinant CD4.
We studied 684 sera obtained from 20 hemophilia patients with AIDS/AIDS-related complex (ARC), 89 asymptomatic HIV+, 76 HIV- hemophilia patients and 151 healthy controls for antibodies against recombinant CD4 (rCD4). Twenty-two percent of AIDS/ARC patients, 10% of asymptomatic HIV+ patients, 17% of HIV-patients, and 1% of healthy controls had anti-rCD4 antibodies. Purified anti-rCD4 antibodies did not react with human CD4+ lymphocytes. This may explain why formation of anti-rCD4 antibodies correlated neither with the occurrence of autoantibodies against CD4+ lymphocytes nor with a decrease in CD4+ cell counts. Antibodies that were eluted from CD4+ lymphocytes after sequential adsorption and elution with separated CD8+ and CD4+ cells reacted with CD4+ lymphocytes of only some healthy individuals, suggesting diversity of CD4 expression.
Daniel
V
V
Department of Transplantation Immunology, University of Heidelberg, FRG.
Weimer
R
R
Zettlmeissl
G
G
Langner
K
K
Zimmermann
R
R
Opelz
G
G
D000163:Acquired Immunodeficiency Syndrome / Q000276:immunology; D000327:Adsorption; D000937:Antigen-Antibody Reactions / Q000276:immunology; D001323:Autoantibodies / Q000097:blood*; D015704:CD4 Antigens / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D000939:Epitopes / Q000276:immunology; D015488:HIV Antigens / Q000276:immunology; D015658:HIV Infections / Q000150:complications / Q000635:transmission*; D006467:Hemophilia A / Q000150:complications / Q000276:immunology*; D006801:Humans; D007141:Immunoglobulin Fc Fragments / Q000276:immunology; D015728:Receptors, HIV / Q000276:immunology; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology; D065227:Transfusion Reaction
D001323:Autoantibodies; D015704:CD4 Antigens; D000939:Epitopes; D015488:HIV Antigens; D007141:Immunoglobulin Fc Fragments; D015728:Receptors, HIV