title: Splenic primary sensory afferents in the guinea pig demonstrated with anterogradely transported wheat-germ agglutinin conjugated to horseradish peroxidase.
abstract: The distribution of primary visceral afferents to the spleen of the guinea pig was studied after injections of wheat-germ agglutinin conjugated to horseradish peroxidase (HRP) into the left dorsal root ganglia at levels T7-T12. After anterograde transport of the tracer, labeled fibers were found in the nerves around the splenic artery in the hilus region and in the splenic parenchyma. The majority of labeled fibers in the spleen were detected in the white pulp. HRP-positive fibers were also observed in the red pulp and in the trabeculae. The distribution of the HRP-labeled fibers was in part similar to those of substance P-immunoreactive and calcitonin gene-related peptide-immunoreactive nerve structures. The results show that the anterograde tracing technique can be used successfully to investigate splenic primary afferent innervation.
authors.affiliation: Department of Anatomy, Karolinska Institutet, Stockholm, Sweden.
authors.forename: L G
authors.initials: LG
authors.lastname: Elfvin
authors.forename: H
authors.initials: H
authors.lastname: Aldskogius
authors.forename: J
authors.initials: J
authors.lastname: Johansson
mesh_terms: D000344:Afferent Pathways / Q000033:anatomy & histology / Q000378:metabolism; D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D006168:Guinea Pigs; D006735:Horseradish Peroxidase / Q000378:metabolism; D007150:Immunohistochemistry; D008297:Male; D008854:Microscopy, Electron; D013154:Spleen / Q000294:innervation*; D013373:Substance P / Q000378:metabolism; D018998:Wheat Germ Agglutinin-Horseradish Peroxidase Conjugate; D014909:Wheat Germ Agglutinins / Q000378:metabolism
chemical_list: D018998:Wheat Germ Agglutinin-Horseradish Peroxidase Conjugate; D014909:Wheat Germ Agglutinins; D013373:Substance P; D006735:Horseradish Peroxidase; D015740:Calcitonin Gene-Related Peptide