Induction of cystatin S in rat submandibular glands by papain.
1. Papain (a cysteine proteinase) were administered into the oral cavity of rats twice daily for 5 days. This treatment caused a dramatic increase in the level of cystatin S (a cysteine proteinase inhibitor belonging to family 2 of cystatin superfamily) in enlarged submandibular glands. 2. Immunochemical analysis using antibody against rat cystatin S and electrophoretic analysis confirmed that the protein induced by papain was identical to that induced by isoproterenol. 3. Induction of the cystatin S in the submandibular glands by oral administration of papain suggested a biological response which plays a role in preventing injury exogenous proteinase.
Naito
Y
Y
Department of Biological Chemistry, Faculty of Pharmaceutical Sciences, Nagoya City University, Japan.
Suzuki
I
I
Hasegawa
S
S
D000818:Animals; D015153:Blotting, Western; D015891:Cystatins / Q000032:analysis / Q000096:biosynthesis*; D004591:Electrophoresis, Polyacrylamide Gel; D008297:Male; D008790:Metoprolol / Q000494:pharmacology; D010206:Papain / Q000378:metabolism*; D011506:Proteins / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D055333:Salivary Cystatins; D013363:Submandibular Gland / Q000187:drug effects* / Q000378:metabolism
C525174:Cyss protein, rat; D015891:Cystatins; D011506:Proteins; D055333:Salivary Cystatins; D010206:Papain; D008790:Metoprolol