title: [Chemical-enzymatic synthesis and cloning in Escherichia coli of a gene coding for human granulocyte-colony stimulating factor].
abstract: Two artificial genes, encoding two forms of human granulocyte colony stimulating factor as products of a normal and an alternative splicing, have been by a chemical-enzymatic way synthesized and cloned in Escherichia coli. The genes are supplied with recognition sites of restriction endonucleases to facilitate the further cassette mutagenesis.
authors.forename: S K
authors.initials: SK
authors.lastname: Kash'ian
authors.forename: N S
authors.initials: NS
authors.lastname: Bystrov
authors.forename: E F
authors.initials: EF
authors.lastname: Boldyreva
authors.forename: I A
authors.initials: IA
authors.lastname: Poliakova
authors.forename: I V
authors.initials: IV
authors.lastname: Severtsova
authors.forename: V G
authors.initials: VG
authors.lastname: Korobko
mesh_terms: D000595:Amino Acid Sequence; D001345:Autoradiography; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA; D004262:DNA Restriction Enzymes; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D005813:Genes, Synthetic; D016179:Granulocyte Colony-Stimulating Factor / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D010957:Plasmids; D012326:RNA Splicing
chemical_list: D016179:Granulocyte Colony-Stimulating Factor; D004247:DNA; D004262:DNA Restriction Enzymes