[Immunohistochemical analysis of physicochemical properties of target antigens with anti-tumor monoclonal antibodies].
A new simplified assay for the identification of physicochemical nature of the antigen recognized by monoclonal antibodies (MAbs) against tumor using direct immunohistochemical methods of avidin-biotin peroxidase complex (ABC) is first described. The experimental results demonstrate that this method does not need to purify the target antigens or special equipment, but is as specific and sensitive as immunoblotting technique. For these reasons, this procedure is much simpler and less expensive than the methods previously published. This method may thus be useful to investigate the properties of antigens to which MAbs are directed.
Liu
M P
MP
PLA General of Hospital Shenyang Command, Liaoning.
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm / Q000032:analysis* / Q000737:chemistry; D015179:Colorectal Neoplasms / Q000276:immunology*; D004938:Esophageal Neoplasms / Q000276:immunology; D005770:Gastrointestinal Neoplasms / Q000276:immunology; D006801:Humans; D007124:Immunoenzyme Techniques; D010190:Pancreatic Neoplasms / Q000276:immunology; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm