A mutational hotspot in the aprt gene of Chinese hamster cells.
Early work with adenine phosphoribosyltransferase-deficient mutants of CHO cells suggested that a site existed in the third exon of this gene which was preferentially susceptible to mutation by ethyl methanesulphonate. To determine whether this was real we analysed a large collection of induced mutants, and generated a high-density mutational spectrum for this exon. In addition, 4 sites outside exon 3 were analysed by blot. 37 mutations were found in 19 available sites, six of which were at nucleotide 1365, 1 of 2 sites in the putative hotspot (P less than 0.02). One other site, 1308, also was mutated in 6 cell lines and may also be preferentially mutable.
Belouchi
A
A
Institut du Cancer de Montréal, Que., Canada.
Bradley
W E
WE
D000228:Adenine Phosphoribosyltransferase / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D016466:CHO Cells; D006224:Cricetinae; D008969:Molecular Sequence Data; D009154:Mutation
D000228:Adenine Phosphoribosyltransferase