title: Induction of MHC antigens by tumour cell lines in response to interferons.
abstract: The induction of major histocompatibility complex antigens by interferons (IFN) on 17 established tumour cell lines was investigated by radio binding. One bladder (Fen) and two testis lines (Tera I and Ha) lacked class I antigens and IFN-gamma failed to induce their expression. However, IFN-gamma upregulated these antigens on lines expressing low class I antigens (Tera II and EP2102) with little or no significant effect on high class I expressing lines (T24 and RT112). In one bladder line (Wil) IFN-gamma, whilst failing to alter monomorphic class I, upregulated polymorphic HLA-A2 and A3 antigens. None of the 17 lines expressed class II antigens, but could all be induced by IFN-gamma except T24, TccSup, Tera II and Lan lines. This defect was not due to the absence of IFN-gamma receptor, since under the same conditions intracellular adhesion molecule 1 was upregulated. IFN-alpha, whilst failing to have any effect on class II, induced class I antigens. IFN-beta showed no activity on either class I or II antigens when used alone. However, in combination, it inhibited IFN-gamma induced class II antigens. Thus, it may be possible to study cells from fresh tumours to preselect the minority of patients who might benefit from cytokine therapy.
authors.affiliation: Department of Medical Oncology, Royal London Hospital, U.K.
authors.forename: A M
authors.initials: AM
authors.lastname: Nouri
authors.forename: R F
authors.initials: RF
authors.lastname: Hussain
authors.forename: A V
authors.initials: AV
authors.lastname: Dos Santos
authors.forename: D J
authors.initials: DJ
authors.lastname: Gillott
authors.forename: R T
authors.initials: RT
authors.lastname: Oliver
mesh_terms: D000911:Antibodies, Monoclonal; D001943:Breast Neoplasms / Q000276:immunology*; D002295:Carcinoma, Transitional Cell / Q000276:immunology*; D004407:Dysgerminoma / Q000276:immunology*; D015395:Histocompatibility Antigens Class I / Q000378:metabolism*; D000949:Histocompatibility Antigens Class II / Q000378:metabolism*; D006801:Humans; D007372:Interferons / Q000494:pharmacology*; D013724:Teratoma / Q000276:immunology*; D014407:Tumor Cells, Cultured
chemical_list: D000911:Antibodies, Monoclonal; D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II; D007372:Interferons