title: Enzymatic and immunological detection of G protein alpha-subunits in the pathogenic fungus Candida albicans.
abstract: GTP stimulation of adenylyl cyclase from the dimorphic pathogenic fungus Candida albicans is greatly enhanced by preincubation of membrane proteins with cholera toxin, NAD and ATP. In the presence of [32P]NAD the toxin catalyzes the covalent incorporation of radioactivity into a membrane protein of 40 kDa. Pertussis toxin catalyzes the transference of the radioactivity from [32P]NAD to a 32 kDa protein. Two major proteins of 40-42 and 30-32 kDa can also be recognized in Western blots by an anti G alpha-common antibody. The results support the idea that G proteins are part of the hormone sensory transduction chain of Candida [(1990) Biochem. Biophys. Res. Commun. 167, 1177-1183].
authors.affiliation: Departamento de Quimica Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina.
authors.forename: C
authors.initials: C
authors.lastname: Paveto
authors.forename: L
authors.initials: L
authors.lastname: Montero
authors.forename: S
authors.initials: S
authors.lastname: Passeron
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D037361:Adenylate Cyclase Toxin; D000262:Adenylyl Cyclases / Q000187:drug effects / Q000378:metabolism; D015153:Blotting, Western; D002176:Candida albicans / Q000737:chemistry* / Q000201:enzymology; D002772:Cholera Toxin / Q000494:pharmacology; D019204:GTP-Binding Proteins / Q000032:analysis* / Q000276:immunology; D006160:Guanosine Triphosphate / Q000378:metabolism; D008565:Membrane Proteins / Q000187:drug effects / Q000378:metabolism; D009243:NAD / Q000378:metabolism; D037342:Pertussis Toxin; D011065:Poly(ADP-ribose) Polymerases / Q000187:drug effects / Q000378:metabolism; D010566:Virulence Factors, Bordetella / Q000494:pharmacology
chemical_list: D037361:Adenylate Cyclase Toxin; D008565:Membrane Proteins; D010566:Virulence Factors, Bordetella; D009243:NAD; D006160:Guanosine Triphosphate; D000255:Adenosine Triphosphate; D002772:Cholera Toxin; D011065:Poly(ADP-ribose) Polymerases; D037342:Pertussis Toxin; D019204:GTP-Binding Proteins; D000262:Adenylyl Cyclases