title: Helicobacter pylori secretes a chemotactic factor for monocytes and neutrophils.
abstract: Helicobacter pylori is associated with an inflammatory reaction in the stomach and duodenum, yet the mechanism of this inflammatory infiltrate is unknown. The ability of Helicobacter pylori to secrete a factor that attracts leucocytes is investigated. Helicobacter pylori conditioned supernatant attracted neutrophils and monocytes with 50-100% of the activity of control chemotactic factor, 10(-8) M formyl-methionine-leucine-phenylalanine. Strains derived from individuals with ulcer or non-ulcer associated H pylori infections displayed similar chemotactic activity. Preliminary characterisation shows that the factor has a molecular weight of less than 3000, is heat stable, is acid resistant, and can be diluted at least 10-fold. Checkerboard analysis confirmed that the activity was chemotactic rather than chemokinetic. This chemotactic activity could play a role in the pathogenesis of Helicobacter pylori gastritis.
authors.affiliation: Department of Medicine, University of California, Los Angeles.
authors.forename: P M
authors.initials: PM
authors.lastname: Craig
authors.forename: M C
authors.initials: MC
authors.lastname: Territo
authors.forename: W E
authors.initials: WE
authors.lastname: Karnes
authors.forename: J H
authors.initials: JH
authors.lastname: Walsh
mesh_terms: D002630:Chemotactic Factors / Q000032:analysis / Q000096:biosynthesis*; D002634:Chemotaxis, Leukocyte; D016480:Helicobacter pylori / Q000378:metabolism*; D006801:Humans; D008970:Molecular Weight; D009000:Monocytes; D009240:N-Formylmethionine Leucyl-Phenylalanine; D009504:Neutrophils; D013997:Time Factors
chemical_list: D002630:Chemotactic Factors; D009240:N-Formylmethionine Leucyl-Phenylalanine