HIV-1 env, nef, and gag-specific T-cell immunity in mice: conserved epitopes in nef p27 and gag p25 proteins.
Cellular immunogenicity of env gp160, nef p27, and gag p55 proteins of human immunodeficiency virus type 1 (HIV-1) was studied in mice immunized with vaccinia virus recombinants. Proliferative responses of spleen cells were comparable against env gp160, nef p27, and gag p25 recombinant proteins. No specific activity was observed against gag p18 protein. Env, nef, and gag-specific T-cell lines were generated by repeated stimulation of immune spleen cells with recombinant HIV-1 proteins. They were CD4 positive, proliferative, and also cytotoxic against HIV-transfected target cells. Specificity of the T-cell response against nef and gag protein was analyzed with synthetic peptides. Peptides nef 15, nef 16, and gag AM-30 were, respectively, reactive in nef- and gag-specific proliferative and cytolytic assays. The three peptides described have a relatively conserved amino acid sequence among HIV isolates and appear broadly immunoreactive among species.
Michel
F
F
Laboratoire d'Immunopathologie des Rétrovirus, Institut Pasteur, Paris, France.
Hoffenbach
A
A
Froussard
P
P
Langlade-Demoyen
P
P
Kaczorek
M
M
Kieny
M P
MP
Plata
F
F
D000595:Amino Acid Sequence; D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology*; D002455:Cell Division; D000090266:Chromobox Protein Homolog 5; D000939:Epitopes; D015686:Gene Products, env / Q000276:immunology*; D015683:Gene Products, gag / Q000276:immunology*; D015693:Gene Products, nef / Q000276:immunology*; D015488:HIV Antigens / Q000276:immunology; D019302:HIV Envelope Protein gp160; D015497:HIV-1 / Q000276:immunology*; D007111:Immunity, Cellular; D051379:Mice; D008969:Molecular Sequence Data; D011498:Protein Precursors / Q000276:immunology*; D013154:Spleen / Q000166:cytology; D014765:Viral Vaccines / Q000008:administration & dosage; D054301:gag Gene Products, Human Immunodeficiency Virus; D054311:nef Gene Products, Human Immunodeficiency Virus
C000715594:CBX5 protein, human; D000939:Epitopes; D015686:Gene Products, env; D015683:Gene Products, gag; D015693:Gene Products, nef; D015488:HIV Antigens; D019302:HIV Envelope Protein gp160; C059827:HIV gag protein p25; D011498:Protein Precursors; D014765:Viral Vaccines; D054301:gag Gene Products, Human Immunodeficiency Virus; D054311:nef Gene Products, Human Immunodeficiency Virus; D000090266:Chromobox Protein Homolog 5