title: Concentrations of major plasma proteins in serum and whole-tissue extracts of porcine fetuses during development.
abstract: In extracts from fetuses up to 32 days of gestation, the major serum proteins were fetuin, alpha-fetoprotein and alpha 1-antitrypsin, but albumin was not detected. The concentration of all proteins rose with age until 40-50 days of gestation; and then the serum concentration of alpha-fetoprotein (2.9 mg ml-1), alpha 1-antitrypsin (4.4 mg ml-1) and transferrin (2.6 mg ml-1) fell progressively to about 1 mg ml-1 at birth, whereas those of fetuin, albumin and alpha 1-acid glycoprotein increased. The patterns of serum proteins in fetuses at about the middle of gestation were similar in extracts and sera. At birth, the major proteins were alpha 1-acid glycoprotein and fetuin, which accounted for 45 and 18% of serum proteins, respectively. Albumin represented only 7% of serum proteins at this age. For most of the second gestational period, the six quantified proteins accounted for about 85% of total serum proteins. In early gestation, a significant proportion of serum proteins was intracellular.
authors.affiliation: Departmento de Bioquímica y Biología Molecular y Celular, Facultad de Ciencias, Universidad de Zaragoza, Spain.
authors.forename: F
authors.initials: F
authors.lastname: Lampreave
authors.forename: A
authors.initials: A
authors.lastname: Piñeiro
mesh_terms: D000818:Animals; D001797:Blood Protein Electrophoresis; D001798:Blood Proteins / Q000032:analysis*; D005314:Embryonic and Fetal Development / Q000502:physiology*; D005333:Fetus / Q000098:blood supply / Q000737:chemistry*; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D007150:Immunohistochemistry; D009961:Orosomucoid / Q000032:analysis; D012709:Serum Albumin / Q000032:analysis; D013552:Swine / Q000097:blood / Q000196:embryology*; D014168:Transferrin / Q000032:analysis; D000515:alpha 1-Antitrypsin / Q000032:analysis; D000509:alpha-Fetoproteins / Q000032:analysis
chemical_list: D001798:Blood Proteins; D009961:Orosomucoid; D012709:Serum Albumin; D014168:Transferrin; D000515:alpha 1-Antitrypsin; D000509:alpha-Fetoproteins