[The immunological characterization of 63 cases of Hodgkin's disease with a panel of 8 antibodies].
Sixty three cases of Hodgkin's disease are studied (two with lymphonodular predominance, 15 with diffuse lymphocyte predominance, 26 nodular sclerosis, 15 mixed cell and 5 lymphocyte depletion) with a panel of 8 monoclonal antibodies, material routinely used and included in paraffin: Ber H2 (CD30), Leu M1 (CD15), Common Leukocyte Antigen (CD45), L26 (CD20), MB2, UCHL1 (CD45 RO), MTI (CD43) and Epithelial Membrane Antigen. Ber H2 turned out to be the most usefull marker, positive in 100% of cases, independently of the histologic type. Positiveness with Leu M1 ranged from 100% (2/2 cases) of lymphonodular predominance, to 53.3% (8/15 cases) of diffuse lymphocyte predominance. The reactivity of the rest was variable, 1 though it is note worthy the positiveness of B markers (L26, MB2) in the two cases with lymphonodular predominance. In the other subtypes, reactivity with L26 was greater than that with MB2. T cell marker expression was minimal, except for the positiveness in 40% of cases (2/5) of the lymphocyte depletion type. In addition, the results of other series are revised and as a result the possible histogenesis of Hodgkin's disease is discussed.
Oliva-Aldamiz
H
H
Departamento de Anatomía Patológica, Fundación Jiménez Díaz, Universidad Autónoma de Madrid.
Echezarreta
G
G
Aguilera
B
B
Cuevas
E
E
Barat
A
A
Sarasa
J L
JL
Renedo
G
G
Rivas
F
F
Forteza
J
J
Sánchez-Fayos
M P
MP
D000911:Antibodies, Monoclonal; D006689:Hodgkin Disease / Q000175:diagnosis / Q000276:immunology*; D006801:Humans; D007150:Immunohistochemistry; D008198:Lymph Nodes / Q000276:immunology; D016539:Reed-Sternberg Cells / Q000276:immunology; D013194:Staining and Labeling
D000911:Antibodies, Monoclonal