Determination of N-acetylator phenotype using caffeine as a probe compound: a comparison of high-performance liquid chromatography and capillary electrophoresis methods.
A test for determining N-acetylator metabolic phenotype has been developed using caffeine as a probe drug. A spot sample of urine is taken, and the unextracted urine is then analysed by micellar electrokinetic capillary chromatography. Phenotype is determined from the peak-area ratio of urinary 5-acetylamino-6-formylamino-3-methyluracil to 1-methylxanthine. Phenotype assignments using this method were compared with those made using a standard high-performance liquid chromatography assay, with good agreement between the two methods. The advantage of the capillary electrophoresis analysis is that no sample extraction is necessary, resulting in a total analysis time of around 20 min, and removing a potential source of error.
Lloyd
D K
DK
Department of Oncology, McGill University, Montreal, Quebec, Canada.
Fried
K
K
Wainer
I W
IW
D000107:Acetylation; D002110:Caffeine / Q000378:metabolism* / Q000652:urine; D002851:Chromatography, High Pressure Liquid; D004586:Electrophoresis / Q000379:methods; D006801:Humans; D010641:Phenotype; D012016:Reference Values; D015203:Reproducibility of Results
D002110:Caffeine