title: Localization of myosin IIB at the leading edge of growth cones from rat dorsal root ganglionic cells.
abstract: Primary cultures of rat dorsal root ganglionic (DRG) cells were stained with isoform-specific antibodies against non-muscle myosin II. Antibodies against the brain type myosin (MIIB) stained the peripheries of growth cones and non-neuronal cells. Double staining of the cells with the anti-myosin antibodies and rhodamine-phalloidin or anti-actin antibodies indicated that MIIB co-exists, with F-actin, at the leading edge. Antibodies against platelet myosin stained neither leading edges nor neurites, but stained the cell bodies of neurons and the stress fibers of non-neuronal cells. These results suggest that MIIB functions in the motility of the leading edge of DRG cells.
authors.affiliation: Department of Anatomy and Cell Biology, SUNY, HSC, Brooklyn.
authors.forename: T P
authors.initials: TP
authors.lastname: Cheng
authors.forename: N
authors.initials: N
authors.lastname: Murakami
authors.forename: M
authors.initials: M
authors.lastname: Elzinga
mesh_terms: D000818:Animals; D002455:Cell Division; D002478:Cells, Cultured; D005455:Fluorescent Antibody Technique; D005727:Ganglia, Spinal / Q000737:chemistry* / Q000166:cytology; D009218:Myosins / Q000032:analysis*; D016501:Neurites / Q000737:chemistry*; D009474:Neurons / Q000737:chemistry* / Q000166:cytology; D051381:Rats
chemical_list: D009218:Myosins