Acute phase proteins but not activated microglial cells are present in parenchymal beta/A4 deposits in the brains of patients with hereditary cerebral hemorrhage with amyloidosis-Dutch type.
With immunohistochemical staining methods on cryostat sections we investigated the brains of three patients with hereditary cerebral hemorrhage with amyloidosis-Dutch type, one of the cerebral beta/A4 amyloid diseases. Immunostaining for beta/A4 protein revealed numerous non-fibrillar beta/A4 depositions (amorphous or diffuse plaques) in the brain parenchyma in addition to extensive vascular amyloid deposition. All amorphous plaques contain complement proteins and alpha 1-antichymotrypsin but activated microglial cells expressing major histocompatibility (MHC) class II antigens HLA-DR and leucocyte adhesion molecules belonging to the lymphocyte-function-associated antigen (LFA)-1 family are virtually absent in cortical gray matter. Our findings are discussed from the view that a cascade of events including acute phase proteins and activated microglial cells are involved in classical amyloid plaque formation.
Rozemuller
J M
JM
Department of Psychiatry, Free University of Amsterdam, Netherlands.
Bots
G T
GT
Roos
R A
RA
Eikelenboom
P
P
D000209:Acute-Phase Proteins / Q000032:analysis*; D000328:Adult; D016229:Amyloid beta-Peptides / Q000032:analysis*; D016564:Amyloid beta-Protein Precursor; D000686:Amyloidosis / Q000378:metabolism / Q000473:pathology*; D002540:Cerebral Cortex / Q000737:chemistry / Q000473:pathology; D002543:Cerebral Hemorrhage / Q000378:metabolism / Q000473:pathology*; D003165:Complement System Proteins / Q000032:analysis; D006684:HLA-DR Antigens / Q000032:analysis; D006801:Humans; D016169:Lymphocyte Function-Associated Antigen-1 / Q000032:analysis; D008264:Macrophages / Q000473:pathology; D008875:Middle Aged; D009457:Neuroglia / Q000473:pathology*; D011498:Protein Precursors / Q000032:analysis*; D011956:Receptors, Cell Surface / Q000032:analysis*; D000514:alpha 1-Antichymotrypsin / Q000032:analysis
D000209:Acute-Phase Proteins; D016229:Amyloid beta-Peptides; D016564:Amyloid beta-Protein Precursor; D006684:HLA-DR Antigens; D016169:Lymphocyte Function-Associated Antigen-1; D011498:Protein Precursors; D011956:Receptors, Cell Surface; D000514:alpha 1-Antichymotrypsin; D003165:Complement System Proteins