Mammalian GnRH involvement in prostaglandin F2 alpha and sex steroid hormones testicular release in two amphibian species: the anuran water frog, Rana esculenta, and the urodele crested newt, Triturus carnifex.
The present work was carried out to study the in vitro effects of mammalian gonadotropin-releasing hormone (mGnRH) on Rana esculenta and Triturus carnifex testis production of prostaglandin F2 alpha (PGF2 alpha) and sex steroid hormones during the prereproduction, reproduction, and postreproduction periods. In R. esculenta, testicular PGF2 alpha release was lower during postreproduction, and mGnRH increased PGF2 alpha in prereproduction and reproduction. Androgens were higher during prereproduction, and mGnRH induced an androgens increase in prereproduction and reproduction. In T. carnifex testicular PGF2 alpha release was lower during reproduction, and mGnRH increased PGF2 alpha in prereproduction and reproduction. Androgens were higher in reproduction and lower in postreproduction, and mGnRH induced an androgens increase in reproduction. Estradiol-17 beta release was higher in postreproduction, and mGnRH induced an estradiol decrease in reproduction and an increase in postreproduction. These results seem to indicate the involvement of PGF2 alpha in the testicular reproductive activity, and a similar mGnRH mechanism of action, both in R. esculenta and in T. carnifex. In addition, taken together with previous studies, they seem to suggest that the relationship found between mGnRH and PGF2 alpha or sex steroids could be widespread in amphibians.
Gobbetti
A
A
Department of Molecular, Cellular, Animal Biology, University of Camerino, Italy.
Zerani
M
M
D000728:Androgens / Q000378:metabolism; D000818:Animals; D015237:Dinoprost / Q000378:metabolism*; D012739:Gonadal Steroid Hormones / Q000378:metabolism*; D007987:Gonadotropin-Releasing Hormone / Q000494:pharmacology*; D066298:In Vitro Techniques; D008297:Male; D010507:Periodicity; D011374:Progesterone / Q000378:metabolism; D011863:Radioimmunoassay; D011893:Rana esculenta / Q000502:physiology*; D012098:Reproduction / Q000502:physiology; D012455:Salamandridae / Q000502:physiology*; D012680:Sensitivity and Specificity; D013737:Testis / Q000378:metabolism*; D013997:Time Factors
D000728:Androgens; D012739:Gonadal Steroid Hormones; D007987:Gonadotropin-Releasing Hormone; D011374:Progesterone; D015237:Dinoprost