title: Eukaryotic cells grown on microcarrier beads offer a cost-efficient way to propagate Chlamydia trachomatis.
abstract: The use of microcarrier cell culture as a method for the in vitro propagation of the obligate intracellular bacterial parasite, Chlamydia trachomatis, is described. The microcarrier beads proved to be a more cost-effective means to propagate C. trachomatis than traditional tissue culture flasks or roller bottles without sacrificing yields or infectivity. In addition, microcarrier cell culture was found to be a much simpler technique to study the intracellular development of these bacteria.
authors.affiliation: Department of Microbiology and Immunology, University of North Carolina, Chapel Hill 27599.
authors.forename: J E
authors.initials: JE
authors.lastname: Tam
authors.forename: S T
authors.initials: ST
authors.lastname: Knight
authors.forename: C H
authors.initials: CH
authors.lastname: Davis
authors.forename: P B
authors.initials: PB
authors.lastname: Wyrick
mesh_terms: D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000096:biosynthesis; D001431:Bacteriological Techniques; D002460:Cell Line; D002692:Chlamydia trachomatis / Q000254:growth & development*; D046508:Culture Techniques / Q000191:economics / Q000295:instrumentation*; D005057:Eukaryotic Cells / Q000166:cytology / Q000382:microbiology*; D007739:L Cells / Q000166:cytology / Q000382:microbiology; D051379:Mice; D008863:Microspheres
chemical_list: D001425:Bacterial Outer Membrane Proteins