Galactose terminating oligosaccharides of IgG in patients with primary Sjögren's syndrome.
Using a simple but novel ELISA, we have screened 40 serum samples from patients with primary Sjögren's syndrome and 34 normal controls for IgG glycosylation deficiencies, identified by their specific ricin binding. Elevated levels of asialylated IgG were detected in 24 patients. The extent of asialylation was significantly higher in the patients with extraglandular manifestations than in the others. Interestingly, the correlation of asialylated IgG was apparent only with Raynaud's phenomenon and arthritis, and not other extraglandular manifestations. Strong correlations (P less than 0.01) were noted between asialylated IgG and rheumatoid factor or IgA-containing immune complexes.
Youinou
P
P
Laboratory of Immunology, Brest University Medical School, France.
Pennec
Y L
YL
Casburn-Budd
R
R
Dueymes
M
M
Letoux
G
G
Lamour
A
A
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001323:Autoantibodies / Q000737:chemistry* / Q000276:immunology; D001327:Autoimmune Diseases / Q000097:blood / Q000150:complications / Q000276:immunology*; D002240:Carbohydrate Sequence; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005690:Galactose / Q000032:analysis*; D006031:Glycosylation; D006801:Humans; D007070:Immunoglobulin A / Q000276:immunology; D007074:Immunoglobulin G / Q000737:chemistry* / Q000276:immunology; D007132:Immunoglobulin Isotypes / Q000276:immunology; D037102:Lectins / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000276:immunology* / Q000378:metabolism; D037121:Plant Lectins; D011928:Raynaud Disease / Q000209:etiology / Q000276:immunology; D012217:Rheumatoid Factor / Q000097:blood; D012859:Sjogren's Syndrome / Q000097:blood / Q000150:complications / Q000276:immunology*
D001323:Autoantibodies; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007132:Immunoglobulin Isotypes; D037102:Lectins; D009844:Oligosaccharides; D037121:Plant Lectins; C017707:Ricinus communis agglutinin-1; D012217:Rheumatoid Factor; D005690:Galactose