Determination of free calcium.
A computer program designed to be user friendly is described in this report. Two types of calculations are provided: a) determination of free calcium concentration based on known total salt concentration and b) those which calculate total salt concentration required to insure a desired free calcium concentration in the buffered system. The user can choose up to 10 different ligands, nine different cations (known total concentration) and 10 concentrations (either free or total) of the variable cation (e.g. calcium) per calculation. Thus, this program does not restrict its user to one or two parameters (e.g., buffer and/or ions). Data generated using this method are in excellent agreement with those derived using programs in current use.
Taylor
R B
RB
Brain Research Laboratory of Biochemistry, University of Texas, San Antonio 78285.
Trimble
C
C
Valdes
J J
JJ
Wayner
M J
MJ
Chambers
J P
JP
D000465:Algorithms; D002118:Calcium / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D012984:Software
D002118:Calcium