Differences in activation of normal and glycosylphosphatidylinositol-negative lymphocytes derived from patients with paroxysmal nocturnal hemoglobinuria.
In these studies, the role of glycosylphosphatidylinositol (GPI)-anchored surface molecules during T cell activation was investigated in fresh T cells and T cell lines obtained from patients with paroxysmal nocturnal hemoglobinuria. For control, GPI-expressing T cells of the same patients were used. Unstimulated GPI- T cells exhibited significantly reduced surface expression of the activation Ag CD45R0, compared with GPI+ T cells. In addition, in measurements of proliferation, IFN-gamma production, and induction of second messengers such as cytoplasmic Ca2+, CD48- lymphocytes showed a similar response to TCR-specific stimulation, compared with CD48+ lymphocytes. In contrast, stimulation with the lectin PHA produced a decreased response of CD48- lymphocytes in these functions. In addition, stimulation with cross-linked CD59 mAb increased the proliferation of GPI-molecule expressing CD48+ T cell lines only. From these data, it can be concluded that GPI-anchored surface molecules play an important role in T lymphocyte activation.
Schubert
J
J
Abteilung Immunologie und Transfusionsmedizin, Zentrum Innere Medizin, Medizinische Hochschule, Hannover, Germany.
Uciechowski
P
P
Zielinska-Skowronek
M
M
Tietjen
C
C
Leo
R
R
Schmidt
R E
RE
D015703:Antigens, CD / Q000032:analysis / Q000276:immunology; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis; D017252:CD3 Complex; D000071178:CD48 Antigen; D018957:CD59 Antigens; D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D016207:Cytokines / Q000096:biosynthesis; D006017:Glycolipids / Q000502:physiology*; D017261:Glycosylphosphatidylinositols; D006457:Hemoglobinuria, Paroxysmal / Q000276:immunology*; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis; D007376:Interleukin-2 / Q000494:pharmacology; D008213:Lymphocyte Activation; D008562:Membrane Glycoproteins / Q000276:immunology; D010716:Phosphatidylinositols / Q000502:physiology*; D011948:Receptors, Antigen, T-Cell / Q000032:analysis; D015398:Signal Transduction; D016176:T-Lymphocyte Subsets / Q000276:immunology*
D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D017252:CD3 Complex; D000071178:CD48 Antigen; C000604065:CD48 protein, human; D018957:CD59 Antigens; D016207:Cytokines; D006017:Glycolipids; D017261:Glycosylphosphatidylinositols; D007376:Interleukin-2; D008562:Membrane Glycoproteins; D010716:Phosphatidylinositols; D011948:Receptors, Antigen, T-Cell; D007371:Interferon-gamma; D002118:Calcium