Detection of Mycoplasma hyopneumoniae by using rRNA-oligodeoxynucleotide hybridization.
A system that uses rRNA-oligodeoxynucleotide hybridization was developed for the detection of Mycoplasma hyopneumoniae. Synthetic oligonucleotide MHP1 was hybridized specifically with M. hyopneumoniae. Furthermore, the detection of M. hyopneumoniae in clinical samples, such as bronchoalveolar lavage fluid and lung lesions from experimentally infected pigs, was evaluated by this assay. The evidence obtained from the assay indicated that the system can be used to efficiently diagnose mycoplasmal pneumonia of swine. Additionally, a nonradioisotopic system with chemiluminescence detection was tested. This system was 10-fold less sensitive than a test that used radioisotopes.
Futo
S
S
Central Laboratory, Nippon Flour Mills Co., Ltd., Kanagawa, Japan.
Seto
Y
Y
Mitsuse
S
S
Mori
Y
Y
D000818:Animals; D001483:Base Sequence; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D008969:Molecular Sequence Data; D009174:Mycoplasma / Q000235:genetics* / Q000302:isolation & purification; D009175:Mycoplasma Infections / Q000382:microbiology; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes / Q000138:chemical synthesis; D012329:RNA, Bacterial / Q000235:genetics*; D012335:RNA, Ribosomal / Q000235:genetics*; D012680:Sensitivity and Specificity; D013552:Swine
D015345:Oligonucleotide Probes; D012329:RNA, Bacterial; D012335:RNA, Ribosomal