Species-specific restriction of complement by HRF20 (CD59) generated by cDNA transfection.
The 20-kDa homologous restriction factor (HRF20, CD59) is a phosphatidyl inositol-anchored membrane glycoprotein that inhibits the formation of human complement membrane attack complexes. The cDNA of HRF20 was transfected into Chinese hamster ovary (CHO) cells resulting in expression of human HRF20 protein on the cell surface anchored via glycosylphosphatidyl inositol. The transfected CHO cells were resistant to human complement-mediated cell killing. However, the cells remained sensitive to rat and guinea pig complement. Therefore, species specificity between HRF20 and complement is maintained in HRF20 generated on the CHO cells following transfection with HRF20 cDNA.
Takizawa
H
H
Department of Molecular Biology, Nagoya City University School of Medicine, Japan.
Takahashi
K
K
Murakami
T
T
Okada
N
N
Okada
H
H
D000818:Animals; D015703:Antigens, CD / Q000096:biosynthesis* / Q000235:genetics; D018957:CD59 Antigens; D016466:CHO Cells; D003165:Complement System Proteins / Q000502:physiology*; D006224:Cricetinae; D004247:DNA / Q000502:physiology; D006168:Guinea Pigs; D006461:Hemolysis; D006801:Humans; D008562:Membrane Glycoproteins / Q000096:biosynthesis* / Q000235:genetics; D011817:Rabbits; D051381:Rats; D013045:Species Specificity; D014162:Transfection
D015703:Antigens, CD; D018957:CD59 Antigens; D008562:Membrane Glycoproteins; D003165:Complement System Proteins; D004247:DNA