Identification of a src family protein specifically expressed in rat astrocytes by immunohistochemistry and double immunofluorescent study.
Identification of a src-related tyrosine kinase and its regional and cellular distributions were studied in rat brain. The study was performed using a specific antibody raised against a synthetic peptide corresponding to the conserved autophosphorylation site of src-family tyrosine kinases. The antibody (alpha-src antibody) recognized a 43 kDa polypeptide in plasma membrane enriched fraction. The immunohistochemical data revealed that the polypeptide is localized in astrocytes of corpus callosum and fimbria hippocampus. The presence of this src-related protein in astrocytes suggests that it may have some control in their proliferation and differentiation.
Moia
L J
LJ
Department of Physiology, Kagawa Medical School, Japan.
Matsui
H
H
Nishio
H
H
Tsuchida
T
T
Tokuda
M
M
Itano
T
T
Nagao
S
S
Hatase
O
O
D000818:Animals; D001253:Astrocytes / Q000378:metabolism*; D001921:Brain / Q000166:cytology / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D007150:Immunohistochemistry; D016392:Proto-Oncogene Proteins pp60(c-src) / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D014018:Tissue Distribution
D005904:Glial Fibrillary Acidic Protein; D016392:Proto-Oncogene Proteins pp60(c-src)