In vitro synthesis of bacterio-opsin: integration into microsomal membranes.
The translation and membrane integration of bacterio-opsin from Halobacterium salinarium were investigated. Plasmids containing the bacterio-opsin-gene with or without its original presequence were transcribed with the T7-RNA-polymerase and translated in vitro in a wheat germ system. The integration of the expressed bacterio-opsin into dog pancreas microsomes was studied. Both precursor bacterio-opsin and mature bacterio-opsin integrate into the eukaryotic membrane.
Bauer
U
U
Freie Universität Berlin, FB Physik, AG Biophysik, Germany.
Hildebrandt
V
V
Dencher
N A
NA
Wrede
P
P
D000818:Animals; D001436:Bacteriorhodopsins / Q000378:metabolism*; D002417:Cattle; D015870:Gene Expression; D006217:Halobacterium; D066298:In Vitro Techniques; D008565:Membrane Proteins / Q000378:metabolism; D008861:Microsomes / Q000378:metabolism*; D014176:Protein Biosynthesis; D011499:Protein Processing, Post-Translational; D012329:RNA, Bacterial / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism
D008565:Membrane Proteins; D012329:RNA, Bacterial; D012333:RNA, Messenger; D001436:Bacteriorhodopsins; C017827:bacterio-opsin