A simple ICP-MS procedure for the determination of total mercury in whole blood and urine.
A simple and sensitive procedure for total mercury in whole blood and urine using inductively coupled plasma-mass spectrometry (ICP-MS) is described. Specimens are prepared by precipitation-extraction with 50% v/v hydrochloric acid containing EDTA and cysteine, centrifuged, and filtered through fritended screening column; the filtrates are directly analyzed by ICP-MS. The method is linear between 2 and 200 micrograms/L in the specimen with an absolute sensitivity of 0.2 microgram/L in the final supernatant. The assay variability at various concentrations (microgram/L) of mercury are as follows: intra-assay whole blood (n = 20)-4.6 +/- 0.6 (c.v. 12.3%), 18.3 +/- 1.1 (c.v. 6.1%), 56.4 +/- 2.8 (c.v. 5.0%); inter-assay whole blood (n = 15)-5.7 +/- 1.0 (c.v. 16.8%), 19.7 +/- 2.7 (c.v. 13.5%), and 50.1 +/- 6.9 (c.v. 13.7%); urine (n = 20)-9.3 +/- 1.2 (c.v. 12.9%), 29.6 +/- 2.2 (c.v. 7.4%). Recovery of organic and inorganic mercury from blood samples ranges from 91.6% to 110.2%. The method is suitable for analysis of total mercury, both organic and inorganic, in whole blood and urine.
Kalamegham
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Department of Pathology, University of Utah Medical Center, Salt Lake City 84108.
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D001128:Argon; D003545:Cysteine; D004492:Edetic Acid; D006801:Humans; D006851:Hydrochloric Acid; D013058:Mass Spectrometry / Q000379:methods* / Q000706:statistics & numerical data; D008628:Mercury / Q000097:blood* / Q000652:urine*; D008767:Methylmercury Compounds / Q000097:blood / Q000652:urine; D012680:Sensitivity and Specificity
D008767:Methylmercury Compounds; D001128:Argon; D004492:Edetic Acid; D008628:Mercury; D003545:Cysteine; D006851:Hydrochloric Acid