DNA-protein interaction analysis using polyacrylamide gel electrophoresis and a simple and sensitive UV crosslinking procedure.
A simple and reproducible technique for DNA-protein interaction analysis is described using UV crosslinking and polyacrylamide gel electrophoresis, leading to visualization of the complexes as distinct and strong signals. It avoids incorporation of bromodeoxyuridine (BrdU) into DNA and requires no special equipment. It was successfully applied to an oligonucleotide sequence from within the first intron of the mouse myb proto-oncogene and nuclear extracts from a myb-expressing cell line.
Dooley
S
S
Institute of Human Genetics, University of the Saar, Homburg, Germany.
Welter
C
C
Blin
N
N
D001483:Base Sequence; D004268:DNA-Binding Proteins / Q000032:analysis* / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D008970:Molecular Weight; D000090063:Proto-Oncogene Mas; D012680:Sensitivity and Specificity; D014466:Ultraviolet Rays
D004268:DNA-Binding Proteins; C000715470:MAS1 protein, human; D046911:Macromolecular Substances; D000090063:Proto-Oncogene Mas