Identification of Chlamydia trachomatis antigens by use of murine T-cell lines.
Chlamydia-specific short-term T-cell lines were used in conjunction with immunoblot techniques to examine Chlamydia trachomatis proteins for T-cell-stimulatory activity. This study was undertaken because of the known role of T cells in the resolution and pathogenesis of chlamydial infections. Therefore, determination of which chlamydial proteins are T-cell antigens and whether they evoke protective immunity or contribute to immunopathology is crucial. Immune lymph node cells were stimulated with whole chlamydial organism (elementary body) to derive predominantly CD4+ T-cell lines. Proteins from the elementary body and the outer membrane and cloned proteins were examined for antigenicity with these T-cell lines in a proliferation assay. Although a majority of the elementary body protein fractions were positive in this assay, only four of the outer membrane fractions were stimulatory. The cloned major outer membrane protein and outer membrane protein 2 were stimulatory in the assay and may account for the reactivity in three of the four positive outer membrane fractions. The C. trachomatis heat shock protein 60, examined because of its putative role in causing delayed-type hypersensitivity, was found to stimulate the CD4+ T cells. This approach with short-term T-cell lines with polyclonal reactivity was sensitive and specific in identifying chlamydial proteins as T-cell antigens.
Beatty
P R
PR
Department of Biomedical and Environmental Health Sciences, University of California, Berkeley 94720.
Stephens
R S
RS
D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology*; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology; D001426:Bacterial Proteins / Q000276:immunology*; D001483:Base Sequence; D002478:Cells, Cultured; D002690:Chlamydia Infections / Q000276:immunology; D002692:Chlamydia trachomatis / Q000276:immunology*; D066298:In Vitro Techniques; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D016133:Polymerase Chain Reaction; D011993:Recombinant Fusion Proteins / Q000276:immunology; D011994:Recombinant Proteins / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D009838:Oligodeoxyribonucleotides; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins