Site-specific cleavage of glycated human serum albumin in the presence of iron.
Nonenzymatically glycated human serum albumin was incubated with ferric ion at 37 degrees C in 0.2 M phosphate buffer, pH 7.4, up to 30 days. In the incubation mixture, amino acids, tyrosine and phenylalanine, were detected, suggesting site specific cleavage of glycated human serum albumin.
Sakurai
T
T
Tokyo College of Pharmacy, Japan.
Takanashi
M
M
Tsuchiya
S
S
D006801:Humans; D066298:In Vitro Techniques; D007501:Iron / Q000494:pharmacology*; D010649:Phenylalanine / Q000097:blood; D012709:Serum Albumin / Q000378:metabolism*; D014443:Tyrosine / Q000097:blood
D012709:Serum Albumin; D014443:Tyrosine; D010649:Phenylalanine; D007501:Iron