title: Site-specific integration of the Haemophilus influenzae bacteriophage HP1: location of the boundaries of the phage attachment site.
abstract: Plasmids containing DNA segments from the attachment region of phage HP1 were constructed and tested for the ability to replace the phage attachment site substrate in site-specific recombination reactions. The distance separating the boundaries of the functional site was 418 bp. Replacements within the 11-residue segment 5'-GGCGGTTATCG at the left boundary or within the 12-residue segment 5'-GGATTTTTTGAA at the right boundary abolished substrate activity. A segment of the 418-residue sequence preserves the integrity of an operon of three Haemophilus influenzae tRNA genes after HP1 insertion within the coding sequence.
authors.affiliation: Department of Biochemistry, Johns Hopkins University School of Hygiene and Public Health, Baltimore, Maryland 21205.
authors.forename: M A
authors.initials: MA
authors.lastname: Hauser
authors.forename: J J
authors.initials: JJ
authors.lastname: Scocca
mesh_terms: D001287:Attachment Sites, Microbiological / Q000235:genetics*; D001435:Bacteriophages / Q000235:genetics*; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics* / Q000378:metabolism; D004279:DNA, Viral / Q000235:genetics* / Q000378:metabolism; D006193:Haemophilus influenzae / Q000235:genetics*; D008969:Molecular Sequence Data; D009876:Operon; D010957:Plasmids / Q000235:genetics; D012329:RNA, Bacterial / Q000235:genetics; D012343:RNA, Transfer / Q000235:genetics; D011995:Recombination, Genetic / Q000235:genetics
chemical_list: D004269:DNA, Bacterial; D004279:DNA, Viral; D012329:RNA, Bacterial; D012343:RNA, Transfer