title: Down-regulation of scatter factor in MRC 5 fibroblasts by epithelial-derived cells. A model for scatter factor modulation.
abstract: Scatter factor/hepatocyte growth factor (SF/HGF) is a multifunctional cytokine produced by embryonic fibroblasts and other mesenchymal cells that affects the growth and/or the movement of certain epithelia. Here we report that expression of scatter factor activity by MRC 5 cells, a strain of normal human embryonic lung fibroblasts, is greatly reduced as a result of co-culture of these cells with SVK14, an SV40-transformed human keratinocyte cell line. Using a cDNA probe to the beta chain of human HGF, we have found that the fall in SF activity in MRC 5/SVK14 co-cultures is accompanied by the loss of SF/HGF transcripts. As the inhibition of SF activity coincides with the disappearance of SF/HGF transcript, we conclude that inhibition of the SF activity expressed by MRC 5 cells by co-culture with SVK14 involves transcriptional regulation.
authors.affiliation: Biomedical Sciences Division, King's College London, Strand, UK.
authors.forename: T
authors.initials: T
authors.lastname: Kamalati
authors.forename: B
authors.initials: B
authors.lastname: Thirunavukarasu
authors.forename: A
authors.initials: A
authors.lastname: Wallace
authors.forename: N
authors.initials: N
authors.lastname: Holder
authors.forename: R
authors.initials: R
authors.lastname: Brooks
authors.forename: T
authors.initials: T
authors.lastname: Nakamura
authors.forename: M
authors.initials: M
authors.lastname: Stoker
authors.forename: E
authors.initials: E
authors.lastname: Gherardi
authors.forename: L
authors.initials: L
authors.lastname: Buluwela
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000502:physiology; D002450:Cell Communication / Q000502:physiology; D002478:Cells, Cultured; D046508:Culture Techniques / Q000379:methods; D016207:Cytokines / Q000235:genetics / Q000378:metabolism*; D015536:Down-Regulation; D005347:Fibroblasts / Q000378:metabolism*; D006133:Growth Substances / Q000378:metabolism*; D017228:Hepatocyte Growth Factor; D006801:Humans; D015603:Keratinocytes / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D016207:Cytokines; D006133:Growth Substances; D017228:Hepatocyte Growth Factor