A monoclonal antibody (LK-4) which differentiates PLA1 from PLA2 platelet extracts but not intact platelets.
A unique murine monoclonal antibody (LK-4) is described which differentiates PLA1/PLA1 platelet extracts from PLA2/PLA2 and PLA1/PLA2 platelet extracts on solid phase ELISA and immunoblot at the 100kD GPIIIa location, but not on intact platelets. LK-4 reacts equally with intact PLA1/PLA2 and PLA2/PLA2 platelets. Adsorbtion of LK-4 with PLA1/PLA1 platelets results in loss of reactivity for intact platelets as well as platelet extracts on ELISA or immunoblot. LK-4 inhibits platelet aggregation induced by ADP, epinephrine, collagen and thrombin, suggesting reactivity at or near the fibrinogen binding site on GPIIIa. It is suggested the LK-4 reacts with a conformation-induced common epitope for PLA1 and PLA2 on GPIIIa, with loss of this conformation for PLA2 GPIIIa following solubilization with Triton X-100.
Liu
L X
LX
Department of Medicine, New York University Medical Center, N.Y. 10016.
Nardi
M A
MA
Flug
F
F
Karpatkin
S
S
D000328:Adult; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D016824:Antigens, Human Platelet / Q000235:genetics / Q000276:immunology*; D001792:Blood Platelets / Q000737:chemistry / Q000276:immunology*; D002457:Cell Extracts; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000235:genetics / Q000276:immunology*; D005260:Female; D005838:Genotype; D006801:Humans; D007112:Immunity, Maternally-Acquired; D015151:Immunoblotting; D007231:Infant, Newborn; D051379:Mice; D010641:Phenotype; D010974:Platelet Aggregation; D010980:Platelet Membrane Glycoproteins / Q000235:genetics / Q000276:immunology*; D011247:Pregnancy / Q000276:immunology; D011696:Purpura, Thrombocytopenic / Q000151:congenital / Q000276:immunology
D000911:Antibodies, Monoclonal; D016824:Antigens, Human Platelet; D002457:Cell Extracts; D000939:Epitopes; D010980:Platelet Membrane Glycoproteins