DNA fingerprints of Bombyx mori L. Testing of genotypic variability of parthenogenetic strains.
A comparative analysis of the total cellular DNA in certain parthenogenetic specimens of the silkworm (Bombyx mori), produced from the females of the parthenogenetic strains by two types of parthenogenesis, has been performed through the application of the DNA fingerprinting method based on M13 phage DNA as a hybridization probe. It has been shown that parental specimens and their genetically identical off-springs produced through ameiotic parthenogenesis have identical patterns of hybridization with the hypervariable DNA fragments. The off-springs produced through the meiotic type of parthenogenesis have individual-specific patterns of hybridization, revealing a high level of polymorphism of individual genotypes. The results obtained testify to the effectiveness and reliability of this promising method for identification of genotypic variability, marking and genomic characterization of parthenogenetic clones in the silkworm.
Tretjk
A P
AP
V.I. Lenin Moscow Pedagogical State University, Russia.
Ryskov
A P
AP
Sevastyanova
G A
GA
Filippovich YuB
Strunnikov
V A
VA
D000818:Animals; D012831:Bombyx / Q000235:genetics*; D016172:DNA Fingerprinting; D015342:DNA Probes; D019003:Deoxyribonuclease HpaII; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D005260:Female; D014644:Genetic Variation; D005838:Genotype; D010312:Parthenogenesis / Q000235:genetics
D015342:DNA Probes; D019003:Deoxyribonuclease HpaII; D015252:Deoxyribonucleases, Type II Site-Specific