Specific polypeptide markers in chronic B cell malignancies detected by two-dimensional gel electrophoresis.
Two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) analysis of silver stained polypeptides was used to discriminate the cell protein profiles in chronic B cell malignancies. We identified 5 distinct polypeptides (H1 to H5) present in hairy cell leukemia (HCL) (14 cases) and absent in most chronic lymphocytic leukemia (CLL) and normal B lymphocytes. Two polypeptides, H2 and H5, were absolutely specific of HCL. The 2-D PAGE profiles found in 3 splenic lymphoma with villous lymphocytes (SLVL) and 2 HCL variants (HCL-V) were similar to HCL with a unique additional peptide present in 3/3 SLVL and 1/2 HCL-V. When HCL and SLVL were assessed for the CLL stage-specific 2-D PAGE patterns, HCL was related to stage A/B while SLVL was related to stage C. In conclusion, 2-D PAGE analysis of the molecular pattern of B lymphocytes provides a new means to recognize the leukemic origin of the sample and distinguish HCL from CLL and SLVL.
Talebian-Ziai
S
S
Départment d'Hématologie, CHU Pitié Salpêtrière, Paris, France.
Azgui
Z
Z
Valensi
F
F
Sigaux
F
F
Charron
D
D
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001402:B-Lymphocytes / Q000737:chemistry; D014408:Biomarkers, Tumor / Q000097:blood*; D015180:Electrophoresis, Gel, Two-Dimensional; D000939:Epitopes / Q000032:analysis; D005260:Female; D006801:Humans; D007943:Leukemia, Hairy Cell / Q000097:blood*; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000097:blood; D016393:Lymphoma, B-Cell / Q000097:blood*; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000302:isolation & purification; D010455:Peptides / Q000097:blood*; D016622:Silver Staining; D013160:Splenic Neoplasms / Q000097:blood*
D014408:Biomarkers, Tumor; D000939:Epitopes; D009363:Neoplasm Proteins; D010455:Peptides