Inhibition of class II MHC gene expression by anti-sense RNA in transgenic mice.
We have established transgenic mice carrying the anti-sense DNA to the gene encoding beta chain of the class II major histocompatibility complex (I-A) molecule. The amount of I-A molecule on splenic B lymphocytes from the mice was reduced in the presence of a large amount of the exogenous anti-sense RNA. The amount of I-A beta chain RNA was selectively reduced and inversely correlated with the amount of anti-sense RNA in the spleens. These results suggest that the I-A beta chain RNA is rapidly degraded by duplex formation with the anti-sense RNA in splenic B cells from the transgenic mice.
Miki
N
N
Department of Immunology, Kobe University School of Medicine, Japan.
Tokuhisa
T
T
D000818:Animals; D000911:Antibodies, Monoclonal; D016373:DNA, Antisense / Q000235:genetics*; D015870:Gene Expression / Q000187:drug effects; D005802:Genes, MHC Class II / Q000187:drug effects*; D000949:Histocompatibility Antigens Class II / Q000032:analysis / Q000235:genetics; D046911:Macromolecular Substances; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D015347:RNA Probes; D016372:RNA, Antisense / Q000235:genetics / Q000494:pharmacology*; D015183:Restriction Mapping; D012260:Ribonucleases; D013154:Spleen / Q000276:immunology
D000911:Antibodies, Monoclonal; D016373:DNA, Antisense; D000949:Histocompatibility Antigens Class II; D046911:Macromolecular Substances; D015347:RNA Probes; D016372:RNA, Antisense; D012313:RNA; D012260:Ribonucleases