A coagglutination assay with monoclonal antibodies for rapid laboratory identification of Mycoplasma synoviae.
An agglutinating monoclonal antibody (MAb S2) specific for a 55,000-molecular-weight surface protein of Mycoplasma synoviae was developed by fusion of spleen cells from immunized BALB/c mice with P3X63 Ag8.653 myeloma cells. Immunogold labeling experiments confirmed the cell surface location of the MAb-recognized antigen. MAb S2-coated Staphylococcus aureus was used in a rapid slide coagglutination assay. Eleven M. synoviae strains, including the type strain WVU 1853, coagglutinated with MAb-coated S. aureus, but five M. gallisepticum strains (PG31, S6, R, F, and A5969) did not.
Morsy
M A
MA
Department of Pathobiology, College of Veterinary Medicine, Auburn University, Alabama.
Panangala
V S
VS
Gresham
M M
MM
Toivio-Kinnucan
M
M
D000372:Agglutination Tests; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D000942:Antigens, Bacterial / Q000276:immunology*; D000954:Antigens, Surface / Q000276:immunology; D015153:Blotting, Western; D000939:Epitopes / Q000276:immunology; D006825:Hybridomas; D051379:Mice; D016253:Microscopy, Immunoelectron; D008970:Molecular Weight; D009174:Mycoplasma / Q000276:immunology / Q000302:isolation & purification* / Q000648:ultrastructure; D013045:Species Specificity
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D000954:Antigens, Surface; D000939:Epitopes