Solid-phase extraction of prostanoids using an automatic sample preparation system.
A commercial automated solid-phase extraction system for cyclooxygenase arachidonic acid metabolites in urine samples has been evaluated. Comparison of manual and automatic batch (36 samples) extraction procedures for tritium labelled prostanoids added as tracers to urine samples has shown equivalent results with recoveries greater than 90% for prostaglandins E2, F2alpha and 6-keto prostaglandin F1alpha as well as thromboxane B2. Analyte stability is not affected by the automated procedure, which uses less solvents and has a faster overall processing time than the manual method. The automated system has been applied to the extraction of prostanoids in urine samples from workers exposed to dichloroethane.
Hotter
G
G
Department of Neurochemistry, Eicosanoid Branch, Centro de Investigación y Desarrollo, CSIC, Barcelona, Spain
Gómez
G
G
Ramis
I
I
Bioque
G
G
Roselló-Catafau
J
J
Gelpi
E
E
D001095:Arachidonic Acids / Q000652:urine; D001322:Autoanalysis; D002851:Chromatography, High Pressure Liquid; D005025:Ethylene Dichlorides / Q000633:toxicity; D006801:Humans; D016273:Occupational Exposure; D011453:Prostaglandins / Q000302:isolation & purification* / Q000652:urine
D001095:Arachidonic Acids; D005025:Ethylene Dichlorides; D011453:Prostaglandins