title: Expression of 120 kilodalton protein and cytotoxicity in Helicobacter pylori.
abstract: Antral biopsy culture supernatants from 14 subjects with chronic gastritis, known to have IgA antibodies to the 120 kilodalton protein, showed positive recognition of this antigen in western blots against a cytotoxin positive strain of Helicobacter pylori but gave negative reactions with two cytotoxin negative strains. Control immunoblots with culture supernatants from 13 non-responders to the protein were all negative. This indicates a direct association between expression of the 120 kilodalton protein in H pylori strains and cytotoxicity.
authors.affiliation: Department of Medicine, St James's University Hospital, Leeds.
authors.forename: J E
authors.initials: JE
authors.lastname: Crabtree
authors.forename: N
authors.initials: N
authors.lastname: Figura
authors.forename: J D
authors.initials: JD
authors.lastname: Taylor
authors.forename: M
authors.initials: M
authors.lastname: Bugnoli
authors.forename: D
authors.initials: D
authors.lastname: Armellini
authors.forename: D S
authors.initials: DS
authors.lastname: Tompkins
mesh_terms: D000368:Aged; D000942:Antigens, Bacterial / Q000737:chemistry / Q000276:immunology*; D001426:Bacterial Proteins / Q000737:chemistry / Q000276:immunology*; D015153:Blotting, Western; D003603:Cytotoxins / Q000276:immunology; D005756:Gastritis / Q000276:immunology*; D016480:Helicobacter pylori / Q000276:immunology*; D006801:Humans; D007070:Immunoglobulin A / Q000276:immunology; D008875:Middle Aged; D008970:Molecular Weight
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D003603:Cytotoxins; D007070:Immunoglobulin A