Modulation of ganglioside expression in human melanoma cell lines; increased resistance to chemo- and radiation treatment.
Cell surface gangliosides in human melanoma cell lines were modulated by pretreatment and adaptation to 6-thioguanine and 5-bromo-deoxyuridine. Chemo- and radiation sensitivities were compared in original cell lines and modulated cells by the human tumor colony-forming assay. Modulated cells showed decreased expression of cell surface GM2 and GD2 gangliosides. This reduction was correlated with increased resistance to bleomycin, vincristine, cisplatin and radiation treatment. These results suggest that cell surface GM2 and GD2 ganglioside expression in human melanoma cells is intimately associated with several cellular biological properties, such as drug or radiation sensitivity and cellular differentiation.
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Division of Surgical Oncology, Jhon Wayne Cancer Clinic, University of California school of Medicine, Los Angeles 90024.
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D000970:Antineoplastic Agents / Q000494:pharmacology*; D001761:Bleomycin / Q000494:pharmacology; D001973:Bromodeoxyuridine / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects* / Q000528:radiation effects*; D002855:Chromatography, Thin Layer; D002945:Cisplatin / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004307:Dose-Response Relationship, Radiation; D004351:Drug Resistance; D005732:Gangliosides / Q000302:isolation & purification / Q000378:metabolism*; D006801:Humans; D008545:Melanoma; D008563:Membrane Lipids / Q000302:isolation & purification / Q000378:metabolism*; D015122:Mercaptopurine / Q000494:pharmacology; D014407:Tumor Cells, Cultured; D014410:Tumor Stem Cell Assay; D014750:Vincristine / Q000494:pharmacology
D000970:Antineoplastic Agents; D005732:Gangliosides; D008563:Membrane Lipids; D001761:Bleomycin; D014750:Vincristine; D015122:Mercaptopurine; D001973:Bromodeoxyuridine; D002945:Cisplatin