title: Molecular cloning and immunological analysis of immunodominant piroplasm surface proteins of Theileria sergenti and T. buffeli.
abstract: cDNA libraries of Theileria sergenti and T. buffeli piroplasms were constructed in lambda gt11 and screened with rabbit anti-piroplasm sera. A major antigen of T. sergenti (33 kDa) and that of T. buffeli (34 kDa) was identified from the recombinant phages by using recombinant antigen-selected monospecific antibodies. The reactivities of the cloned proteins with rabbit antisera, infected calf sera and mouse monoclonal antibody suggested that the 33 and 34 kDa proteins expressed species-common and species-specific epitopes. The DNA probes from these recombinant clones showed species-specific hybridizations in Southern blotting with genomic DNA from piroplasms. These results indicate that the Japanese T. sergenti can be distinguishable from the Australian T. buffeli with regard to a polymorphism of the major immunodominant proteins of piroplasm.
authors.affiliation: National Institute of Animal Health, Ibaraki, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Kawazu
authors.forename: C
authors.initials: C
authors.lastname: Sugimoto
authors.forename: T
authors.initials: T
authors.lastname: Kamio
authors.forename: K
authors.initials: K
authors.lastname: Fujisaki
mesh_terms: D000818:Animals; D000953:Antigens, Protozoan / Q000235:genetics* / Q000276:immunology; D000954:Antigens, Surface / Q000235:genetics / Q000276:immunology; D015139:Blotting, Southern; D015153:Blotting, Western; D002417:Cattle; D003001:Cloning, Molecular; D003429:Cross Reactions; D016054:DNA, Protozoan / Q000032:analysis*; D000939:Epitopes / Q000276:immunology; D015723:Gene Library; D007106:Immune Sera / Q000276:immunology; D015800:Protozoan Proteins / Q000235:genetics* / Q000276:immunology; D016053:RNA, Protozoan / Q000235:genetics*; D016794:Theileria / Q000235:genetics / Q000276:immunology*
chemical_list: D000953:Antigens, Protozoan; D000954:Antigens, Surface; D016054:DNA, Protozoan; D000939:Epitopes; D007106:Immune Sera; D015800:Protozoan Proteins; D016053:RNA, Protozoan