Determination of retinyl palmitate and total vitamin A content in liver and liver-based ready-to-eat foods.
The determination of retinyl palmitate and total vitamin A in liver and liver-based ready-to-eat foods is described. The method is very simple as sample preparation is minimal, and the isocratic elution of the C18 column with pure methanol does not necessitate a sophisticated instrumental set-up. The method is accurate with high recoveries (100.6 +/- 9.3%, mean +/- S.D., n = 23), and precise with within-day and between-day coefficients of variation of less than 5.5% (n = 13) and less than 16% (n = 6), respectively.
Pikkarainen
S A
SA
Department of Clinical Chemistry, University of Kuopio, Finland.
Parviainen
M T
MT
D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004224:Diterpenes; D008099:Liver / Q000737:chemistry*; D008461:Meat Products / Q000032:analysis*; D015203:Reproducibility of Results; D000084562:Retinyl Esters; D013056:Spectrophotometry, Ultraviolet; D013552:Swine; D014801:Vitamin A / Q000031:analogs & derivatives* / Q000032:analysis*
D004224:Diterpenes; D000084562:Retinyl Esters; D014801:Vitamin A; C014794:retinol palmitate