Myristoylation-dependent membrane targeting and release of the HTLV-I Gag precursor, Pr53gag, in yeast.
The unprocessed HTLV-I Gag precursor, Pr53gag, was synthesized in yeast, Saccharomyces cerevisiae. The synthesized Pr53gag was myristoylated, associated with the cellular membrane, and released into the culture medium. The released Pr53gag was pelleted by centrifugation at 100,000 x g for 2 h. Conversion of Gly2 to Ala allowed synthesis of a non-myristoylated soluble Pr53gag which was not released into the culture medium. These results suggest that the release of the HTLV-I Gag precursors Pr53gag, occurs in yeast in a myristoylation-dependent manner.
Hayakawa
T
T
Biology Research Laboratories, Takeda Chemical Industries, Ltd., Japan.
Miyazaki
T
T
Misumi
Y
Y
Kobayashi
M
M
Fujisawa
Y
Y
D000595:Amino Acid Sequence; D001483:Base Sequence; D001692:Biological Transport; D002462:Cell Membrane / Q000737:chemistry; D002498:Centrifugation; D003001:Cloning, Molecular; D003470:Culture Media; D004279:DNA, Viral; D015683:Gene Products, gag / Q000235:genetics / Q000378:metabolism*; D015368:Human T-lymphotropic virus 1 / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D009227:Myristic Acids / Q000378:metabolism*; D010957:Plasmids; D011498:Protein Precursors / Q000235:genetics / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*
D003470:Culture Media; D004279:DNA, Viral; D015683:Gene Products, gag; D009227:Myristic Acids; D011498:Protein Precursors