Fine-scale deletion mapping of the distal long arm of chromosome 6 in 70 human ovarian cancers.
To define a small region on chromosome 6q containing a putative tumor suppressor gene for ovarian cancer, we examined loss of heterozygosity in 70 ovarian tumors of three histological types with nine restriction fragment length polymorphism markers located at 6q24-27. Among 33 cancers of serous type that were informative at one or more loci, 17 showed allelic loss at a few or all loci examined, whereas only 1 of 15 mucinous-type tumors and 2 of 12 clear-cell tumors revealed loss of heterozygosity. This result supported our earlier suggestion that alteration of a gene on chromosome 6q may play an important role during development of serous ovarian tumors (Sato et al., Cancer Res., 51: 5118-5122, 1991). Frequent losses were observed between loci defined by CI6-119 (D6S195) at 6q26 and CI6-49 (D6S161) at 6q27. A detailed deletion map indicated a commonly deleted region between loci defined by CI6-111 (D6S193) and CI6-24 (D6S149); these two markers are estimated to be 1.9 cM apart on the basis of linkage analysis. Our results further define a region containing a tumor suppressor gene involved in ovarian carcinoma within an approximately 2-megabase-long segment of chromosome 6q.
Saito
S
S
Department of Biochemistry, Cancer Institute, Tokyo, Japan.
Saito
H
H
Koi
S
S
Sagae
S
S
Kudo
R
R
Saito
J
J
Noda
K
K
Nakamura
Y
Y
D015139:Blotting, Southern; D002874:Chromosome Mapping / Q000379:methods*; D002896:Chromosomes, Human, Pair 6 / Q000737:chemistry*; D005260:Female; D017353:Gene Deletion; D006579:Heterozygote; D006801:Humans; D010051:Ovarian Neoplasms / Q000737:chemistry / Q000235:genetics*