title: Induction in Gallus domesticus of experimental hypercholesterolemia by saturated fat. Effects on cholesterogenic enzyme activity.
abstract: The effect of coconut oil supplementation to the diet (10 or 20%) on lipid levels in plasma and liver as well as on the cholesterogenic enzyme activity were studied in 14-day-old chicks. Treatments for 1 or 2 weeks did not interfere in the growth rate of animals nor in the liver weight. The 10% coconut oil group showed a significant increase of plasma cholesterol after 2 weeks of treatment, while after 1 week the increase was not statistically significant. The 20% coconut oil group increased plasma cholesterol from the first week. Triacylglycerol content increased after each coconut oil supplementation to the diet during the first week. Hepatic cholesterol did not change significantly after any treatment assayed. No significant difference was observed in the cholesterogenic activity, measured as hepatic 3-hydroxy-3-methylglutaryl-CoA reductase, so that this study provides a perfect model of hypercholesterolemic animals without changes in their cholesterogenic ability.
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Granada, Spain.
authors.forename: M F
authors.initials: MF
authors.lastname: Zafra
authors.forename: M
authors.initials: M
authors.lastname: Castillo
authors.forename: F
authors.initials: F
authors.lastname: Rodriguez-Vico
authors.forename: E
authors.initials: E
authors.lastname: Garcia-Peregrin
mesh_terms: D000818:Animals; D002645:Chickens; D002784:Cholesterol / Q000097:blood / Q000378:metabolism; D000074263:Coconut Oil; D004041:Dietary Fats; D006903:Hydroxymethylglutaryl CoA Reductases / Q000378:metabolism*; D006937:Hypercholesterolemia / Q000139:chemically induced*; D008099:Liver / Q000201:enzymology* / Q000378:metabolism; D010938:Plant Oils / Q000008:administration & dosage*; D014280:Triglycerides / Q000097:blood / Q000378:metabolism
chemical_list: D004041:Dietary Fats; D010938:Plant Oils; D014280:Triglycerides; D002784:Cholesterol; D006903:Hydroxymethylglutaryl CoA Reductases; D000074263:Coconut Oil