Interleukin-5 increases the expression of alkaline phosphatase activity in murine B lymphocytes.
Interleukin-5 (IL-5) was shown to enhance, in a dose-dependent fashion, the expression of alkaline phosphatase (APase) activity in splenic B cells stimulated with dextran sulphate (DXS). The potentiating effect of IL-5 was still more evident when assayed in large B cells than in small resting B cells, whereas IL-2, IL-4 and IL-6 were devoid of activity. Concomitant with increased APase expression, cell-cycle analysis by flow cytometry showed that large B cells in the early G1 phase were stimulated by IL-5, in conjunction with DXS, to enter G1B and to progress further through S and G2/M. A phosphorylation-dephosphorylation pathway could, thus, be involved in IL-5 transmembrane signalling.
Souvannavong
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V
Institut de Biochimie, Université Paris-Sud, Orsay, France.
Brown
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S
Adam
A
A
D000251:Adenosine Triphosphatases / Q000096:biosynthesis*; D000818:Animals; D001402:B-Lymphocytes / Q000187:drug effects* / Q000201:enzymology; D002453:Cell Cycle / Q000187:drug effects; D016264:Dextran Sulfate / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D005260:Female; D005434:Flow Cytometry; D015848:Interleukin-5 / Q000494:pharmacology*; D051379:Mice; D011994:Recombinant Proteins / Q000494:pharmacology; D015398:Signal Transduction / Q000187:drug effects; D013154:Spleen / Q000166:cytology
D015848:Interleukin-5; D011994:Recombinant Proteins; D016264:Dextran Sulfate; D000251:Adenosine Triphosphatases