Altered cell tropism and cytopathicity of feline immunodeficiency viruses in two different feline CD4-positive, CD8-negative cell lines.
Two interleukin-2-dependent feline CD4-positive and CD8-negative cell lines, MYA-1 and the newly established FeL-039, were used as host cells for infection with feline immunodeficiency virus (FIV). All FIV strains used, the Petaluma strain and several new isolates, were highly cytopathic to MYA-1. In contrast, the kinetics of FIV replication in FeL-039 differed greatly depending on the strain tested, i.e., noninfectious strain, highly cytopathic strain, and less cytopathic strain producing a persistent state for a long period. It appears, therefore, that cell tropism for FIV differed with each FIV strain tested even in T-cell lines showing similar cell surface phenotypes. Cytopathicity of FIV is evidently due to both the FIV strain and the host T cell.
Tokunaga
K
K
Section of Serology, Hokkaido University, Sapporo, Japan.
Nishino
Y
Y
Oikawa
H
H
Ishihara
C
C
Mikami
T
T
Ikuta
K
K
D000818:Animals; D015704:CD4 Antigens; D016827:CD8 Antigens; D002415:Cats; D002460:Cell Line; D003588:Cytopathogenic Effect, Viral; D016089:Immunodeficiency Virus, Feline / Q000472:pathogenicity*; D007963:Leukocytes, Mononuclear / Q000382:microbiology; D008297:Male; D012194:RNA-Directed DNA Polymerase / Q000096:biosynthesis; D016176:T-Lymphocyte Subsets / Q000382:microbiology* / Q000473:pathology; D014764:Viral Proteins / Q000096:biosynthesis; D014774:Virulence; D014779:Virus Replication
D015704:CD4 Antigens; D016827:CD8 Antigens; D014764:Viral Proteins; D012194:RNA-Directed DNA Polymerase