Induced crossing-over in Drosophila melanogaster germ cells of DNA repair-proficient and repair-deficient (mei-9L1) males following larval feeding with 5-azacytidine and mitomycin C.
The effects of 5-azacytidine (5-AZ) and mitomycin C (MMC), administered by larval feeding, on crossing-over were measured in Drosophila melanogaster male germ cells of a DNA repair-proficient and a repair-deficient (mei-9L1) strain. Both 5-AZ and MMC are effective inducers of male crossing-over. The estimated number of induced recombination events was higher in repair-proficient than in mei-9L1 males. The apparently lower sensitivity of mei-9L1 males to crossing-over induction may be the result of an incomplete crossing-over process.
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Dipartimento di Genetica, Biologia Generale e Molecolare, Università Federico II, Naples, Italy.
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D000818:Animals; D001374:Azacitidine / Q000494:pharmacology*; D003434:Crossing Over, Genetic / Q000187:drug effects*; D004260:DNA Repair / Q000187:drug effects*; D004331:Drosophila melanogaster / Q000235:genetics; D005854:Germ Cells / Q000187:drug effects*; D007814:Larva / Q000187:drug effects; D008297:Male; D016685:Mitomycin / Q000494:pharmacology*; D011995:Recombination, Genetic / Q000187:drug effects
D016685:Mitomycin; D001374:Azacitidine