Enumeration of interleukin-1 alpha and beta producing cells by flow cytometry.
A technique for intracytoplasmic immunofluorescence staining to detect and quantify human interleukin-1 alpha (IL-1 alpha) and beta (IL-1 beta) in CD4, CD8, and CD14 positive lymphoid cells is described. Mononuclear cells stimulated in vitro with PHA to produce IL-1, were fixed and made permeable to antibodies by sequential exposure to paraformaldehyde and the detergent n-octyl-glucoside. Cytoplasmic and surface staining of both forms of IL-1 were demonstrated by indirect fluorescence using IL-1 beta and IL-1 alpha specific mouse monoclonal antibodies and quantified with flow cytometry.
Chikanza
I C
IC
Rheumatology Unit, UMDS of Guy's Hospital, London, UK.
Corrigal
V
V
Kingsley
G
G
Panayi
G S
GS
D015703:Antigens, CD / Q000032:analysis; D015214:Antigens, Differentiation, Myelomonocytic / Q000032:analysis; D015496:CD4-Positive T-Lymphocytes / Q000378:metabolism; D016827:CD8 Antigens / Q000032:analysis; D002469:Cell Separation; D003593:Cytoplasm / Q000378:metabolism; D005434:Flow Cytometry; D006801:Humans; D007375:Interleukin-1 / Q000096:biosynthesis*; D018950:Lipopolysaccharide Receptors; D016131:Lymphocyte Subsets / Q000166:cytology / Q000378:metabolism*
D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D016827:CD8 Antigens; D007375:Interleukin-1; D018950:Lipopolysaccharide Receptors