[DNA sequence of surface antigen gene gp195 of two Chinese isolates of Plasmodium falciparum from Hainan].
Amplification of DNA sequence of surface antigen gene, block II of gp195 from 2 Chinese isolates of Plasmodium falciparum FCC 7801/HN B3 and FCC M21/HN from Hainan, China, was established by using the polymerase chain reaction. A comparison of the 154 peptide sequences of the 2 Chinese isolates showed that they were identical, but there was a difference of 10 peptides among the established 154 peptides between the two Chinese isolates and MAD 20 (a Papua New Guinea isolate). The need to determine the fragments having protective effect against malarial infection, and to study the genetic basis of the antigen polymorphism of Chinese strains of Plasmodium falciparum is evident.
Guan
W B
WB
Department of Parasitology, Second Military Medical University, Shanghai.
Walliker
D
D
Ranford-Cartwright
L
L
Zhang
L Q
LQ
Qu
D
D
D000595:Amino Acid Sequence; D000818:Animals; D000953:Antigens, Protozoan / Q000235:genetics*; D000954:Antigens, Surface / Q000235:genetics*; D001483:Base Sequence; D002681:China; D016054:DNA, Protozoan / Q000235:genetics*; D005796:Genes / Q000235:genetics; D008969:Molecular Sequence Data; D010963:Plasmodium falciparum / Q000145:classification / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D015800:Protozoan Proteins / Q000235:genetics
D000953:Antigens, Protozoan; D000954:Antigens, Surface; D016054:DNA, Protozoan; D015800:Protozoan Proteins