Immunoreactivity, epitope mapping and protection studies with anti-conotoxin GI sera and various conotoxins.
Goat or rabbit anti-conotoxin GI sera recognized native or dithiothreitol-reduced alpha-conotoxins GI, MI and SI in an enzyme-linked immunosorbent assay (ELISA). Native mu-conotoxin GIIIA or omega-conotoxin GVIA did not react with either anti-conotoxin GI serum in the assay. The goat anti-conotoxin GI serum neutralized 2.5 LD50S of alpha-conotoxins GI or MI in mouse lethal assays, while the rabbit antiserum had little protective capabilities. Epitope mapping of synthesized conotoxin peptide fragments revealed that both anti-conotoxin GI sera recognized linear sequences from five different alpha-conotoxins: GI, GIA, GII, MI and SI. The CCNPAC sequence was optimally recognized by both antisera.
Stiles
B G
BG
Department of Toxinology, United States Army Medical Research Institute of Infectious Diseases, Frederick, MD 21702-5011.
Sexton
F W
FW
D000595:Amino Acid Sequence; D000818:Animals; D001427:Bacterial Toxins / Q000276:immunology; D020916:Conotoxins; D003429:Cross Reactions; D004768:Enterotoxins / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000737:chemistry*; D029968:Escherichia coli Proteins; D005260:Female; D007106:Immune Sera / Q000276:immunology*; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D008978:Mollusk Venoms / Q000737:chemistry / Q000276:immunology*; D009500:Neutralization Tests; D010456:Peptides, Cyclic / Q000737:chemistry / Q000276:immunology*; D011817:Rabbits
D001427:Bacterial Toxins; D020916:Conotoxins; D004768:Enterotoxins; D000939:Epitopes; D029968:Escherichia coli Proteins; D007106:Immune Sera; D008978:Mollusk Venoms; D010456:Peptides, Cyclic; C034151:heat stable toxin (E coli); C046101:conotoxin GIII; C029960:conotoxin GI; C055407:conotoxin GV