Evidence that endothelium-dependent relaxation to histamine in the rat common carotid artery is mediated by EDRF.
The mechanism of histamine-induced relaxation in the isolated rat common carotid artery was analysed. Histamine (3 x 10(-7)-10(-4) mol/l) caused a concentration-dependent relaxation of the artery. Indomethacin, a cyclo-oxygenase inhibitor, and diethylcarbamazine, a lipoxygenase inhibitor, did not affect the relaxant response of the artery to histamine. After removal of the vascular endothelium the histamine-induced relaxation was strongly reduced. Moreover, hemoglobin and methylene blue, inhibitors of endothelium-derived relaxing factor (EDRF), prevented or reversed the relaxant effect of histamine. These findings confirm that the histamine-induced relaxation is to a greater extent endothelium dependent. It is concluded that the endothelium-dependent component in the relaxant response of the rat common carotid artery to histamine results from the release of EDRF.
Krstić
M K
MK
Department of Pharmacology, Faculty of Medicine, Belgrade, Yugoslavia.
Stepanović
R M
RM
Vucković
S M
SM
Krstić
S K
SK
D000818:Animals; D002339:Carotid Arteries / Q000187:drug effects*; D004730:Endothelium, Vascular / Q000502:physiology*; D005260:Female; D006632:Histamine / Q000494:pharmacology*; D008297:Male; D009569:Nitric Oxide / Q000502:physiology*; D051381:Rats; D014664:Vasodilation / Q000187:drug effects*
D009569:Nitric Oxide; D006632:Histamine