Large-scale blood substitute production using a microfluidizer.
Microfluidization has been tested as a way to disperse phospholipids in aqueous hemoglobin solutions. Spherical and stable liposomes of 2 to 3 microns were obtained. Lipid incorporation (up to 85%) and hemoglobin encapsulation (up to 15%) in liposomes have been improved with respect to previous investigations. However, results show that a more efficient dispersion system using lower concentrations of lipid is required to obtain a high liposome hemoglobin concentration (limited actually to 150 g/l) and an economically and biologically suitable process for artificial blood production at large scale.
Vivier
A
A
INRS-Santé, Pointe-Claire, Canada.
Vuillemard
J C
JC
Ackermann
H W
HW
Poncelet
D
D
D001802:Blood Substitutes / Q000302:isolation & purification*; D004355:Drug Stability; D006454:Hemoglobins / Q000302:isolation & purification*; D006801:Humans; D008081:Liposomes / Q000302:isolation & purification*; D008560:Membrane Fluidity; D008854:Microscopy, Electron; D010316:Particle Size; D010743:Phospholipids
D001802:Blood Substitutes; D006454:Hemoglobins; D008081:Liposomes; D010743:Phospholipids