title: Influence of thyroparathyroidectomy and thyroxine replacement on Cu and Zn cellular distribution and on the metallothionein level and induction in rats.
abstract: Thyroid hormones are involved in copper and zinc distribution in rat tissues. We examined the influence of thyroparathyroidectomy (TPTY) and of a replacement therapy by T4 on Cu and Zn organ distribution. MT levels were also measured both in basal conditions and after induction by cadmium. The results confirm that a lack of T4 modified Cu and Zn in serum and tissues. In serum, TPTY increased Cu (+15%) and ceruloplasmin (+18%), and decreased Zn (-18%). In tissues, Cu was altered in liver (+13%), kidney (-24%), heart (-16%) duodenum (-18%), and Zn in liver (+25%) and kidney (-10%). The soluble fractions (100,000 g supernatant) were mainly affected in liver and kidney, and the subcellular fractions in heart and duodenum. MT levels were modified in basal conditions only in liver (+57%) and kidney (-36%). T4 administration partially prevented the effect of TPTY on both elements and MT concentrations. Therefore, no evidence is provided for a direct role of T4 in the metabolism of MT in a way comparable to the effects of glucocorticoids. However, MT could mediate the consequences of TPTY on metal distribution in certain organs, such as liver and kidney.
authors.affiliation: Laboratoire de Pharmacologie, Centre Hospitalier Universitaire, Angers, France.
authors.forename: G
authors.initials: G
authors.lastname: Leblondel
authors.forename: A
authors.initials: A
authors.lastname: Le Bouil
authors.forename: P
authors.initials: P
authors.lastname: Allain
mesh_terms: D000818:Animals; D003300:Copper / Q000097:blood / Q000493:pharmacokinetics*; D004435:Eating; D008297:Male; D008668:Metallothionein / Q000096:biosynthesis / Q000378:metabolism*; D010280:Parathyroid Glands / Q000502:physiology*; D016105:Parathyroidectomy; D051381:Rats; D011919:Rats, Inbred Strains; D013961:Thyroid Gland / Q000502:physiology*; D013963:Thyroid Hormones / Q000494:pharmacology; D013965:Thyroidectomy; D013974:Thyroxine / Q000494:pharmacology*; D014018:Tissue Distribution; D015032:Zinc / Q000097:blood / Q000493:pharmacokinetics*
chemical_list: D013963:Thyroid Hormones; D003300:Copper; D008668:Metallothionein; D015032:Zinc; D013974:Thyroxine