title: Cell-to-cell spread of calcium signals mediated by ATP receptors in mast cells.
abstract: Rat basophilic leukaemia cells, like mast cells from which they are derived, have surface Fc epsilon receptors that trigger secretion of inflammatory mediators when crosslinked. Both GTP-binding proteins and a rise in cytosolic calcium concentration ([Ca2+]i) are implicated in the secretory mechanism. Here we use a video-imaging technique to report that transient rises in [Ca2+]i initiated in an individual cell can spread from cell to cell in a wave-like pattern by means of a secreted intermediate, in the absence of gap-junctional communication. We find that the leukaemia cells, peritoneal mast cells and mucosal mast cells have cell-surface P2-type purinergic receptors that can trigger similar [Ca2+]i transients. We provide evidence that ATP is rapidly released, and that it can amplify [Ca2+]i signals and initial secretory responses during antigen-stimulation of rat basophilic leukaemia cells.
authors.affiliation: Department of Physiology and Biophysics, University of California, Irvine 92717.
authors.forename: Y
authors.initials: Y
authors.lastname: Osipchuk
authors.forename: M
authors.initials: M
authors.lastname: Cahalan
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000502:physiology; D000944:Antigens, Differentiation, B-Lymphocyte / Q000502:physiology; D001681:Biological Assay; D002118:Calcium / Q000502:physiology; D002450:Cell Communication / Q000502:physiology*; D002460:Cell Line; D003080:Cold Temperature / Q000009:adverse effects; D003594:Cytoplasmic Granules / Q000378:metabolism; D004912:Erythrocytes / Q000502:physiology; D005246:Feedback; D008407:Mast Cells / Q000502:physiology*; D051381:Rats; D011961:Receptors, Fc / Q000502:physiology; D017455:Receptors, IgE; D011983:Receptors, Purinergic / Q000502:physiology*; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology*; D013498:Suramin / Q000494:pharmacology; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology
chemical_list: D015703:Antigens, CD; D000944:Antigens, Differentiation, B-Lymphocyte; D011961:Receptors, Fc; D017455:Receptors, IgE; D011983:Receptors, Purinergic; D003189:p-Methoxy-N-methylphenethylamine; D013498:Suramin; D002118:Calcium