title: Molecular diversity of the NMDA receptor channel.
abstract: Two novel subunits of the mouse NMDA receptor channel, the epsilon 2 and epsilon 3 subunits, have been identified by cloning and expression of complementary DNAs. The heteromeric epsilon 1/zeta 1, epsilon 2/zeta 1 and epsilon 3/zeta 1 NMDA receptor channels exhibit distinct functional properties in affinities for agonists and sensitivities to competitive antagonists and Mg2+ block. In contrast to the wide distribution of the epsilon 1 and zeta 1 subunit messenger RNAs in the brain, the epsilon 2 subunit mRNA is expressed only in the forebrain and the epsilon 3 subunit mRNA is found predominantly in the cerebellum. The epsilon 1/zeta 1 and epsilon 2/zeta 1 channels expressed in Xenopus oocytes, but not the epsilon 3/zeta 1 channel, are activated by treatment with 12-O-tetradecanoylphorbol 13-acetate. These findings suggest that the molecular diversity of the epsilon subunit family underlies the functional heterogeneity of the NMDA receptor channel.
authors.affiliation: Department of Neuropharmacology, School of Medicine, Niigata University, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Kutsuwada
authors.forename: N
authors.initials: N
authors.lastname: Kashiwabuchi
authors.forename: H
authors.initials: H
authors.lastname: Mori
authors.forename: K
authors.initials: K
authors.lastname: Sakimura
authors.forename: E
authors.initials: E
authors.lastname: Kushiya
authors.forename: K
authors.initials: K
authors.lastname: Araki
authors.forename: H
authors.initials: H
authors.lastname: Meguro
authors.forename: H
authors.initials: H
authors.lastname: Masaki
authors.forename: T
authors.initials: T
authors.lastname: Kumanishi
authors.forename: M
authors.initials: M
authors.lastname: Arakawa
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000502:physiology; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D015870:Gene Expression; D015640:Ion Channel Gating / Q000187:drug effects; D007473:Ion Channels / Q000737:chemistry* / Q000502:physiology; D051379:Mice; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000235:genetics; D016194:Receptors, N-Methyl-D-Aspartate / Q000737:chemistry* / Q000187:drug effects / Q000502:physiology; D011994:Recombinant Proteins; D016415:Sequence Alignment; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
chemical_list: D007473:Ion Channels; D012333:RNA, Messenger; D016194:Receptors, N-Methyl-D-Aspartate; D011994:Recombinant Proteins; D004247:DNA; D013755:Tetradecanoylphorbol Acetate