In vitro adsorption of a hydrophobic mutagen to gastrointestinal mucus glycoprotein (mucin) and dietary fibre.
The adsorption of mutagens by some dietary fibres has been suggested as one mechanism by which dietary fibres protect against colorectal cancer. It is thought that these dietary fibres carry the mutagen out of the digestive tract, decreasing the effective mutagen concentration to which epithelial cells are exposed. The ability of gastrointestinal mucin to alter the extent to which the hydrophobic mutagen 1,8-dinitropyrene (DNP) adsorbs in vitro onto the insoluble dietary fibre alpha-cellulose, was investigated. It was found that crude and purified human ileal mucins themselves adsorbed DNP and decreased the adsorption of DNP onto alpha-cellulose. Purified mucin which had been treated with trypsin also adsorbed DNP. These studies suggest that in the digestive tract there would be competition for the adsorption of DNP between mucin and insoluble dietary fibres, such as alpha-cellulose. This factor must be considered in predictions about the distribution of hydrophobic, mutagenic carcinogens in the digestive tract and their role in the etiology of colorectal cancer.
Ng
T C
TC
Department of Biochemistry, University of Auckland, New Zealand.
Ferguson
L R
LR
Harris
P J
PJ
Watson
M E
ME
Roberton
A M
AM
D000327:Adsorption; D000818:Animals; D002482:Cellulose / Q000737:chemistry; D015179:Colorectal Neoplasms / Q000209:etiology; D004043:Dietary Fiber / Q000378:metabolism*; D005752:Gastric Mucins / Q000494:pharmacology; D006801:Humans; D007082:Ileum; D009077:Mucins / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology; D009152:Mutagenicity Tests; D009153:Mutagens / Q000378:metabolism*; D011721:Pyrenes / Q000737:chemistry / Q000493:pharmacokinetics*; D012996:Solutions; D013552:Swine; D014357:Trypsin; D014547:Urinary Diversion
D004043:Dietary Fiber; D005752:Gastric Mucins; D009077:Mucins; D009153:Mutagens; D011721:Pyrenes; D012996:Solutions; C033310:1,8-dinitropyrene; D002482:Cellulose; D014357:Trypsin