Structure of the mouse 5-HT1C, 5-HT2 and stomach fundus serotonin receptor genes.
By analysis of the mouse 5-HT1C receptor gene we found that its coding region contains three introns. We next isolated cDNA and genomic clones for the closely related 5-HT2 receptor using a probe derived from the 5-HT1C receptor sequence. This probe also hybridized to an additional gene, called SRL (Serotonin Receptor Like). We have evidence demonstrating that it encodes the stomach fundus 5-HT receptor. Two introns are present within the coding regions of the mouse 5-HT2 receptor gene and the SRL gene at positions which correspond to those of introns in the 5-HT1C receptor gene. This intron distribution is unique and distinguishes these receptors from other members of the family of receptors coupled to G-proteins.
Foguet
M
M
Preclinical Research, Sandoz Pharma Ltd., Basel, Switzerland.
Nguyen
H
H
Le
H
H
Lübbert
H
H
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D015698:Genomic Library; D007438:Introns; D051379:Mice; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D016133:Polymerase Chain Reaction / Q000379:methods; D012194:RNA-Directed DNA Polymerase; D011985:Receptors, Serotonin / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D013270:Stomach / Q000502:physiology*
D009838:Oligodeoxyribonucleotides; D011985:Receptors, Serotonin; D004247:DNA; D012194:RNA-Directed DNA Polymerase