Achievement of complete Bacillus subtilis microcycle sporulation by the addition of S-adenosylmethionine and phospholipids.
In an attempt to find factors that may be responsible for the initiation of sporulation, a system in which the germination and outgrowth phases were separate was applied to Bacillus subtilis. Outgrowth of the germinated spores to only the primary singlet cells was followed in chemically defined medium. Addition of specific metabolites induced the primary singlet cells to sporulate via microcycle sporulation. Experiments are described that led to complete sporulation by the addition of diaminopimelic acid, S-adenosyl-L-methionine and phosphatidylethanolamine.
Petridou
S
S
Department of Biology, Syracuse University, NY.
Slepecky
R A
RA
D001412:Bacillus subtilis / Q000254:growth & development / Q000502:physiology*; D002453:Cell Cycle / Q000187:drug effects; D003470:Culture Media; D003562:Cytidine / Q000494:pharmacology; D003960:Diaminopimelic Acid / Q000494:pharmacology*; D010714:Phosphatidylethanolamines / Q000494:pharmacology; D010743:Phospholipids / Q000096:biosynthesis / Q000494:pharmacology*; D012436:S-Adenosylmethionine / Q000494:pharmacology*; D013171:Spores, Bacterial / Q000502:physiology*
D003470:Culture Media; D010714:Phosphatidylethanolamines; D010743:Phospholipids; D003960:Diaminopimelic Acid; D003562:Cytidine; D012436:S-Adenosylmethionine