RNA substrate binding site in the catalytic core of the Tetrahymena ribozyme.
In catalysis by group I introns, the helix (P1) containing the RNA cleavage site must be positioned next to the guanosine binding site. We have identified a conserved adenine in the catalytic core that contributes to the stability of this arrangement and propose that it accepts a hydrogen bond from a specific 2'-OH in P1. Such base-backbone tertiary interactions may be generally important to the organization of RNA tertiary structure.
Pyle
A M
AM
Howard Hughes Medical Institute, University of Colorado, Boulder 80309.
Murphy
F L
FL
Cech
T R
TR
D000225:Adenine / Q000378:metabolism; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002384:Catalysis; D006151:Guanosine / Q000378:metabolism; D006860:Hydrogen Bonding; D007438:Introns; D008745:Methylation; D008969:Molecular Sequence Data; D016296:Mutagenesis; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides / Q000378:metabolism; D012313:RNA / Q000737:chemistry / Q000378:metabolism*; D016337:RNA, Catalytic / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D013379:Substrate Specificity; D016808:Tetrahymena thermophila / Q000235:genetics*; D013816:Thermodynamics
D009841:Oligonucleotides; D016337:RNA, Catalytic; D006151:Guanosine; D012313:RNA; D000225:Adenine