title: Galanin-induced alteration of electrolyte transport in the rat intestine.
abstract: Effects of rat and porcine galanin on rat intestinal ion transport were examined in vitro. In the rat distal colon, a sustained increase in short-circuit current (Isc) was produced by the serosal addition of rat galanin at a concentration as low as 10(-9) M, and a maximal increment was observed at 10(-7) M. Porcine galanin was approximately 100 times less potent than rat galanin. In the rat jejunum, rat galanin produced only a slight and transient decrease in basal Isc. The response to rat galanin was not influenced by atropine, hexamethonium, or amiloride, but was virtually abolished by tetrodotoxin or furosemide. Rat galanin did not significantly influence the increase in Isc elicited by electrical field stimulation in the rat colon and jejunum. Transmural unidirectional 22Na and 36Cl fluxes in the rat colonic mucosa were measured under short-circuited conditions, and rat galanin significantly decreased net sodium and net chloride absorption. These findings suggest that galanin acts as a secretory modulator in the rat colon via noncholinergic neural transmission.
authors.affiliation: Second Department of Internal Medicine, Osaka University Medical School, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Kiyohara
authors.forename: M
authors.initials: M
authors.lastname: Okuno
authors.forename: H
authors.initials: H
authors.lastname: Ishikawa
authors.forename: T
authors.initials: T
authors.lastname: Nakanishi
authors.forename: Y
authors.initials: Y
authors.lastname: Shinomura
authors.forename: C
authors.initials: C
authors.lastname: Yanaihara
authors.forename: Y
authors.initials: Y
authors.lastname: Matsuzawa
mesh_terms: D000584:Amiloride / Q000494:pharmacology; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D003106:Colon / Q000378:metabolism; D004573:Electrolytes / Q000378:metabolism*; D005665:Furosemide / Q000494:pharmacology; D019004:Galanin; D007413:Intestinal Mucosa / Q000294:innervation / Q000378:metabolism*; D007477:Ions; D007583:Jejunum / Q000378:metabolism; D008297:Male; D009407:Nerve Block; D009424:Nervous System Physiological Phenomena; D009479:Neuropeptides / Q000494:pharmacology; D010455:Peptides / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013552:Swine
chemical_list: D004573:Electrolytes; D007477:Ions; D009479:Neuropeptides; D010455:Peptides; D000584:Amiloride; D005665:Furosemide; D019004:Galanin