Enterohepatic circulation in hamsters with an extracorporeal bile duct.
The present study describes a novel technique for investigations of the enterohepatic circulation in the hamster with an extracorporeal bile duct that allows long-term bile collection in the free-moving animal. The animals recovered for 7 days after the operation before the external loop was cut and bile was collected over a period of 78 h. Under these optimal conditions, initial bile flow (651 +/- 89 microliters per 100 g.h-1) and the secretion rates of biliary lipids were several-fold higher than reported in an earlier study using the acute fistula hamster. Biliary cholesterol secretion amounted to 369 +/- 32 nmol per 100 g.h-1, phospholipid secretion was 2.6 +/- 0.3 mumol per 100 g.h-1, and total bile acid secretion was 31.9 +/- 2.2 mumol per 100 g.h-1. A clearcut diurnal rhythm was demonstrated for bile flow and all biliary constituents. After 9 h the depletion of the bile acid pool was complete and cholic acid synthesis derepressed 1.4-fold from a basal rate of 818 nmol per 100 g.h-1, whereas the derepression of chenodeoxycholic acid synthesis was even less pronounced. Biliary cholesterol output increased 2.2-fold, but the phospholipid secretion was constant during the full experiment. It may be concluded that the technique of an extracorporeal bile duct in the free-moving animal allows studies of bile secretion under optimal conditions. Most likely the bile secretion rates given above approach the physiological rates in the hamster.
Fuchs
M
M
Department of Internal Medicine II, University of Ulm, Germany.
Scheibner
J
J
Hörmann
E
E
Tauber
G
G
Stange
E F
EF
D000818:Animals; D001646:Bile / Q000378:metabolism; D001647:Bile Acids and Salts / Q000378:metabolism; D001652:Bile Ducts / Q000502:physiology* / Q000601:surgery; D001658:Biliary Fistula; D001835:Body Weight; D002635:Chenodeoxycholic Acid / Q000096:biosynthesis / Q000378:metabolism; D002784:Cholesterol / Q000378:metabolism; D019826:Cholic Acid; D002793:Cholic Acids / Q000096:biosynthesis / Q000378:metabolism; D006224:Cricetinae; D004435:Eating; D004764:Enterohepatic Circulation / Q000502:physiology*; D005260:Female; D007700:Kinetics; D008099:Liver / Q000033:anatomy & histology / Q000201:enzymology; D008647:Mesocricetus; D008954:Models, Biological; D010743:Phospholipids / Q000378:metabolism; D013656:Taurocholic Acid / Q000096:biosynthesis / Q000378:metabolism
D001647:Bile Acids and Salts; D002793:Cholic Acids; D010743:Phospholipids; D002635:Chenodeoxycholic Acid; D013656:Taurocholic Acid; D002784:Cholesterol; D019826:Cholic Acid