Depletion of tryptophan is not involved in expression of tryptophanyl-tRNA synthetase mediated by interferon.
Gamma interferon (IFN-gamma) affects tryptophan metabolism by mediating the expression of indoleamine 2,3-dioxygenase and tryptophanyl-tRNA synthetase. In the present study, we investigated the role of indoleamine 2,3-dioxygenase-mediated tryptophan depletion in the induction of tryptophanyl-tRNA synthetase by IFN-gamma. The addition of excess tryptophan to the culture medium did not affect the induction of tryptophanyl-tRNA synthetase by IFN-gamma, indicating that tryptophan degradation is not directly involved in the IFN-gamma-mediated expression of tryptophanyl-tRNA synthetase.
Flohr
T
T
Institute for Medical Microbiology, Medical School Hannover, Germany.
Bange
F C
FC
von Euch
A
A
Kiekenbeck
M
M
Böttger
E C
EC
D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology*; D012333:RNA, Messenger / Q000032:analysis; D014364:Tryptophan / Q000378:metabolism*; D014366:Tryptophan Oxygenase / Q000032:analysis / Q000235:genetics; D014369:Tryptophan-tRNA Ligase / Q000032:analysis*
D012333:RNA, Messenger; D007371:Interferon-gamma; D014364:Tryptophan; D014366:Tryptophan Oxygenase; D014369:Tryptophan-tRNA Ligase