title: In vitro activation of dinitrogenase reductase from the cyanobacterium Anabaena variabilis (ATCC 29413).
abstract: Nitrogenase of the heterocystous cyanobacterium Anabaena variabilis was inactivated in vivo (S. Reich, H. Almon, and P. Böger, FEMS Microbiol. Lett. 34:53-56, 1986). Partially purified and modified (inactivated) dinitrogenase reductase (Fe-protein) of such cells was reactivated by isolated membrane fractions of A. variabilis or of Rhodospirillum rubrum, and acetylene reduction was measured. Reactivation requires ATP, Mg2+, and Mn2+. The activating principle is localized in the heterocyst and was found effective only when prepared from cells exhibiting active nitrogenase. It also restores the activity of modified Fe-protein from R. rubrum.
authors.affiliation: Lehrstuhl für Physiologie und Biochemie Pflanzen, Universität Konstanz, Germany.
authors.forename: I
authors.initials: I
authors.lastname: Böhm
authors.forename: A
authors.initials: A
authors.lastname: Halbherr
authors.forename: S
authors.initials: S
authors.lastname: Smaglinski
authors.forename: A
authors.initials: A
authors.lastname: Ernst
authors.forename: P
authors.initials: P
authors.lastname: Böger
mesh_terms: D000114:Acetylene / Q000032:analysis; D000255:Adenosine Triphosphate / Q000378:metabolism; D017033:Anabaena / Q000201:enzymology*; D017037:Dinitrogenase Reductase / Q000302:isolation & purification / Q000378:metabolism*; D004789:Enzyme Activation; D004793:Enzyme Reactivators / Q000378:metabolism; D066298:In Vitro Techniques; D008274:Magnesium / Q000378:metabolism; D008345:Manganese / Q000378:metabolism; D009591:Nitrogenase / Q000378:metabolism*; D012247:Rhodospirillum rubrum / Q000201:enzymology
chemical_list: D017037:Dinitrogenase Reductase; D004793:Enzyme Reactivators; D008345:Manganese; D000255:Adenosine Triphosphate; D009591:Nitrogenase; D008274:Magnesium; D000114:Acetylene