title: The 80L5C4 epitope overlaps with the homophilic binding site of the cell adhesion molecule gp80 of Dictyostelium.
abstract: The monoclonal antibody (mAb) 80L5C4 is a potent inhibitor of the cell adhesion molecule gp80 of Dictyostelium discoideum. To map the exact location of the epitope recognized by mAb 80L5C4, overlapping hexapeptides were synthesized on plastic pins and the binding p6 mAb 80L5C4 to these peptides was monitored by enzyme-linked immunosorbent assay. The 80L5C4 epitope is mapped to a single hexapeptide sequence GYKLNV, which shares five amino acid residues with the octapeptide sequence YKLNVNDS involved in gp80 homophilic binding. Analogue studies indicate that the hydrophobic residues within this sequence are crucial for antigen recognition.
authors.affiliation: Banting and Best Department of Medical Research, University of Toronto, Ont., Canada.
authors.forename: X F
authors.initials: XF
authors.lastname: Wu
authors.forename: R K
authors.initials: RK
authors.lastname: Kamboj
authors.forename: J
authors.initials: J
authors.lastname: Gariepy
authors.forename: C H
authors.initials: CH
authors.lastname: Siu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000946:Antigens, Fungal / Q000276:immunology*; D001665:Binding Sites / Q000276:immunology; D015815:Cell Adhesion Molecules / Q000276:immunology*; D004023:Dictyostelium / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology*; D005656:Fungal Proteins / Q000276:immunology*; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000276:immunology; D015800:Protozoan Proteins
chemical_list: D000911:Antibodies, Monoclonal; D000946:Antigens, Fungal; D015815:Cell Adhesion Molecules; D000939:Epitopes; D005656:Fungal Proteins; D010446:Peptide Fragments; D015800:Protozoan Proteins; C052291:cell cohesion molecule, Dictyostelium