Photochemical stroke and brain-derived neurotrophic factor (BDNF) mRNA expression.
In situ hybridization and Northern blotting were used to study the expression of brain-derived neurotrophic factor (BDNF) mRNA in the rat brain following photochemical stroke. A focal thrombotic lesion of the sensorimotor cortex was produced by intravenously injecting the light-sensitive dye rose bengal and exposing the skull to a controlled beam of light. Four hours after the light exposure the level of BDNF mRNA was increased in the hippocampus and cortex ipsilateral and perifocal to the lesion. The stroke-induced BDNF mRNA increase was prevented by the non-competitive glutamate receptor blocker dizocilpine (MK-801). The results indicate that the activation of N-methyl-D-aspartate (NMDA)-sensitive glutamate receptors is involved in the stroke-triggered stimulation of BDNF mRNA increase.
Comelli
M C
MC
Fidia Research Laboratories, Abano Terme, Italy.
Seren
M S
MS
Guidolin
D
D
Manev
R M
RM
Favaron
M
M
Rimland
J M
JM
Canella
R
R
Negro
A
A
Manev
H
H
D000818:Animals; D015152:Blotting, Northern; D019208:Brain-Derived Neurotrophic Factor; D002540:Cerebral Cortex / Q000502:physiology / Q000503:physiopathology*; D002561:Cerebrovascular Disorders / Q000139:chemically induced / Q000235:genetics / Q000503:physiopathology*; D015870:Gene Expression; D006624:Hippocampus / Q000502:physiology / Q000503:physiopathology*; D008027:Light; D008297:Male; D009414:Nerve Growth Factors / Q000235:genetics; D009419:Nerve Tissue Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000096:biosynthesis*; D011838:Radiation-Sensitizing Agents; D051381:Rats; D017208:Rats, Wistar; D012016:Reference Values; D012395:Rose Bengal
D019208:Brain-Derived Neurotrophic Factor; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D011838:Radiation-Sensitizing Agents; D012395:Rose Bengal