An increased ability to generate hydrogen peroxide in certain polymorphonuclear leukocytes in neonates.
We performed a quantitative assay of the hydrogen peroxide generation in polymorphonuclear leukocytes using single cell analysis by flow cytometry. The study population included premature neonates (n = 10), term neonates (n = 28) and healthy adults (n = 29). The ability to generate hydrogen peroxide in neonatal polymorphonuclear leukocytes was totally reduced compared with adults, however, some polymorphonuclear leukocytes in neonates demonstrated highly accentuated hydrogen peroxide generation.
Ueda
D
D
Department of Pediatrics, Asahikawa Medical College, Japan.
Sakata
Y
Y
Sasaki
K
K
Azuma
H
H
D000328:Adult; D005434:Flow Cytometry; D006801:Humans; D006861:Hydrogen Peroxide / Q000097:blood*; D007231:Infant, Newborn / Q000097:blood*; D007234:Infant, Premature / Q000097:blood; D009504:Neutrophils / Q000378:metabolism*
D006861:Hydrogen Peroxide