DNA relatedness, karyotyping and gene probing of Candida tropicalis, Candida albicans and its synonyms Candida stellatoidea and Candida claussenii.
Isolates of Candida albicans with varied phenotypes, including sucrose-negative variants (C. stellatoidea, serotypes A and B) and avirulent germ tube-negative forms (C. claussenii) showed significant (greater than 90%) DNA relatedness to classical C. albicans, but insignificant relatedness to C. tropicalis and sucrose-negative C. tropicalis. A transverse alternating-field gel electrophoresis procedure (TAFE) showed discrete karyotype patterns among the phenotypic variants of C. albicans including the sucrose-negative C. stellatoidea. The number of chromosome-sized DNA bands for C. tropicalis (7 bands) were within the range of bands observed for C. albicans (5 to 10 bands). The general DNA-migration pattern for C. albicans appeared distinct from that of C. tropicalis. An aspartyl proteinase (PrA) gene probe from C. albicans hybridized with chromosomal DNA from C. albicans, C. claussenii and C. stellatoidea but not with that from C. tropicalis.
Mahrous
M
M
Laboratory for Microbial and Biochemical Sciences, Georgia State University, Atlanta 30303.
Sawant
A D
AD
Pruitt
W R
WR
Lott
T
T
Meyer
S A
SA
Ahearn
D G
DG
D016282:Aspartic Acid Endopeptidases / Q000235:genetics; D002175:Candida / Q000145:classification* / Q000201:enzymology / Q000235:genetics*; D002176:Candida albicans / Q000201:enzymology / Q000235:genetics*; D015342:DNA Probes; D004271:DNA, Fungal / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D007621:Karyotyping; D009693:Nucleic Acid Hybridization; D010641:Phenotype
D015342:DNA Probes; D004271:DNA, Fungal; D016282:Aspartic Acid Endopeptidases