title: Expression cloning of a cDNA from rabbit kidney cortex that induces a single transport system for cystine and dibasic and neutral amino acids.
abstract: We have isolated a cDNA clone by screening a rabbit kidney cortex cDNA library for expression of sodium-independent transport of L-arginine and L-alanine in Xenopus laevis oocytes. Expressed uptake relates to a single component of sodium-independent transport for dibasic and neutral amino acids. This transport activity resembles the functionally defined system b0,+ and carries cystine and dibasic amino acids with high affinity. The rBAT (b0,+ amino acid transporter-related) mRNA is found mainly in kidney and intestinal mucosa. It encodes a predicted 77.8-kDa protein with only one putative transmembrane domain and seven potential N-glycosylation sites. This protein could either be a constitutive element or a specific activator of system b0,+.
authors.affiliation: Institute of Physiology, University of Zürich, Switzerland.
authors.forename: J
authors.initials: J
authors.lastname: Bertran
authors.forename: A
authors.initials: A
authors.lastname: Werner
authors.forename: M L
authors.initials: ML
authors.lastname: Moore
authors.forename: G
authors.initials: G
authors.lastname: Stange
authors.forename: D
authors.initials: D
authors.lastname: Markovich
authors.forename: J
authors.initials: J
authors.lastname: Biber
authors.forename: X
authors.initials: X
authors.lastname: Testar
authors.forename: A
authors.initials: A
authors.lastname: Zorzano
authors.forename: M
authors.initials: M
authors.lastname: Palacin
authors.forename: H
authors.initials: H
authors.lastname: Murer
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000378:metabolism*; D000599:Amino Acids, Diamino / Q000378:metabolism*; D000621:Aminoisobutyric Acids / Q000378:metabolism; D000818:Animals; D001483:Base Sequence; D001692:Biological Transport; D015152:Blotting, Northern; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D003553:Cystine / Q000378:metabolism*; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D007672:Kidney Cortex / Q000378:metabolism / Q000502:physiology*; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D009865:Oocytes / Q000378:metabolism / Q000502:physiology; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D011817:Rabbits; D051381:Rats; D014982:Xenopus laevis
chemical_list: D000596:Amino Acids; D000599:Amino Acids, Diamino; D000621:Aminoisobutyric Acids; D002352:Carrier Proteins; D008565:Membrane Proteins; C100049:2-aminoisobutyric acid; D003553:Cystine; D012313:RNA; D004247:DNA