title: Interaction of lectins with human IgE: IgE-binding property and histamine-releasing activity of twelve plant lectins.
abstract: We examined the IgE-binding reaction and the histamine-releasing response of basophils to a panel of 12 lectins: concanavalin A (Con A), Lens culinaris hemagglutinin (LcH), Pisum sativum agglutinin (PSA), wheat germ agglutinin (WGA), soybean agglutinin (SBA), Bauhinia purpurea agglutinin (BPA), peanut agglutinin (PNA), Ricinus communis agglutinin I (RCA-I), Lotus tetragonolobus agglutinin (Lotus A), Ulex europeus agglutinin I (UEA-I), phytohemagglutinin E (PHA-E) and phytohemagglutinin L (PHA-L), IgE from allergic patients bound with high affinity to Con A, LcH, PSA, RCA-I and PHA-E, and with lower affinity to WGA, BPA, Lotus A and UEA-I, but they did not bind to SBA, PNA or PHA-L. There was no apparent individual difference in the reactivity of IgE to these lectins between 10 IgE preparations from allergic patients. The binding to these lectins, except Lotus A and UEA-I, were competitively inhibited by the lectin-specific sugars or glycopeptide. Upon stimulation by Con A, LcH, PSA, WGA, RCA-1 and PHA-E, leukocytes from allergic patients showed a significant release of histamine, but cells from IgE-deficient subjects did not respond to these lectins. The histamine-releasing responses by these lectins were also inhibited by specific sugars or glycopeptides.
authors.affiliation: Department of Pediatrics, University of Tsukuba, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Shibasaki
authors.forename: R
authors.initials: R
authors.lastname: Sumazaki
authors.forename: S
authors.initials: S
authors.lastname: Isoyama
authors.forename: H
authors.initials: H
authors.lastname: Takita
mesh_terms: D001491:Basophils / Q000276:immunology; D001667:Binding, Competitive; D050260:Carbohydrate Metabolism; D006020:Glycopeptides / Q000378:metabolism; D006636:Histamine Release / Q000187:drug effects; D006801:Humans; D006967:Hypersensitivity / Q000276:immunology; D007073:Immunoglobulin E / Q000737:chemistry / Q000378:metabolism*; D066298:In Vitro Techniques; D037102:Lectins / Q000378:metabolism*
chemical_list: D006020:Glycopeptides; D037102:Lectins; D007073:Immunoglobulin E