A yeast splicing factor is localized in discrete subnuclear domains.
Digital imaging microscopy has been used to visualize the splicing protein PRP6p and three other yeast nuclear proteins. The results show that PRP6p is uniquely localized to discrete subnuclear regions. A combination of cytological and biochemical assays suggests that these sites can be saturated when the protein is overexpressed and likely correspond to the location of U4/U6 snRNPs. The observations indicate that some splicing components are located in discrete subregions of the yeast nucleus, similar to the situation described for the mammalian nucleus.
Elliott
D J
DJ
Howard Hughes Medical Institute, Waltham, MA.
Bowman
D S
DS
Abovich
N
N
Fay
F S
FS
Rosbash
M
M
D015153:Blotting, Western; D002467:Cell Nucleus / Q000378:metabolism* / Q000648:ultrastructure; D005656:Fungal Proteins / Q000032:analysis / Q000378:metabolism*; D012319:RNA Polymerase II / Q000032:analysis / Q000378:metabolism; D012326:RNA Splicing; D012342:RNA, Small Nuclear / Q000378:metabolism; D017414:Ribonucleoprotein, U4-U6 Small Nuclear; D017415:Ribonucleoprotein, U5 Small Nuclear; D017411:Ribonucleoproteins, Small Nuclear / Q000302:isolation & purification / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D029701:Saccharomyces cerevisiae Proteins
D005656:Fungal Proteins; C065573:PRP6 protein, S cerevisiae; D012342:RNA, Small Nuclear; D017414:Ribonucleoprotein, U4-U6 Small Nuclear; D017415:Ribonucleoprotein, U5 Small Nuclear; D017411:Ribonucleoproteins, Small Nuclear; D029701:Saccharomyces cerevisiae Proteins; D012319:RNA Polymerase II