Spontaneously transformed rat-liver epithelial-cell line producing autocrine and paracrine growth factors.
A spontaneously transformed rat-liver epithelial-cell line that could proliferative in unsupplemented, serum-free medium and be passaged with trypsinization has been established. At the time of writing, the line has undergone more than 100 passages in serum-free culture. This cell line produced an autocrine growth-stimulatory factor (AGSF) and a paracrine growth-stimulatory factor (PGSF). AGSF had a remarkable growth-stimulatory effect on transformed rat-liver epithelial-cells, but had no such effect on non-transformed rat-liver epithelial-cells that could not proliferate in serum-free medium. AGSF did not show a growth-inhibitory or stimulatory effect on normal rat kidney (NRK) cells in monolayer culture, and did not induce anchorage-independent growth in soft-agar culture even with epidermal growth factor (EGF) or transforming growth factor (TGF)-beta. AGSF was protease-sensitive, but heat- and acid-stable. The molecular weight was about 700 Dalton (Da) on size-exclusion chromatography. PGSF showed a growth-stimulatory effect on NRK cells and induced anchorage-independent growth in soft-agar culture without EGF or TGF-beta. On the other hand, PGSF slightly inhibited the growth of the spontaneously transformed rat-liver epithelial cells. PGSF was heat-, protease- and acid-sensitive. The molecular weight was 30 kDa on size-exclusion chromatography.
Narita
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Department of Pathology, Hirosaki University School of Medicine, Japan.
D000818:Animals; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000378:metabolism*; D002850:Chromatography, Gel; D002875:Chromosomes; D003470:Culture Media; D004848:Epithelium / Q000378:metabolism / Q000473:pathology; D006133:Growth Substances / Q000096:biosynthesis* / Q000494:pharmacology; D008099:Liver / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D051381:Rats
D003470:Culture Media; D006133:Growth Substances