Some aspects of perfluorochemical emulsion's interaction with blood.
FDC/FTPA (7:3) emulsions stabilized by procsanol (Emulsion 1) and by procsanol with yolk phospholipids (Emulsion 2) were incubated with the donor plasma. After the incubation during 6 hours of Emulsions 1 and 2 with plasma the 36% and 50% decrease of the cholesterol content in plasma was found. Analysis of the lipid content of lipoproteins after the Emulsion 2 administration to rats (2.5 ml/100 g of weight) revealed the 50% decrease of the cholesterol amount in the HDL fraction at 3 and 24 hours posttransfusion. The ratio cholesterol/total phospholipids in the erythrocyte membrane diminished up to 50% as well. The equal degree of the cholesterol adsorption by emulsion from plasma, HDL of rats and erythrocyte membrane is an evidence of nonspecific interaction of PFC particles with the blood components containing cholesterol.
Tereshina
E V
EV
National Research Institute of Technology of Blood Substitutes and Hormonal Preparations, Moscow, USSR.
Doronina
N N
NN
Afonin
N I
NI
Ozerova
I N
IN
Tcherbacova
I A
IA
D000327:Adsorption; D000818:Animals; D001769:Blood / Q000378:metabolism; D001802:Blood Substitutes / Q000493:pharmacokinetics*; D002784:Cholesterol / Q000097:blood; D004655:Emulsions; D004910:Erythrocyte Membrane / Q000378:metabolism; D005466:Fluorocarbons / Q000493:pharmacokinetics*; D006801:Humans; D066298:In Vitro Techniques; D008074:Lipoproteins / Q000097:blood; D010743:Phospholipids / Q000097:blood; D051381:Rats
D001802:Blood Substitutes; D004655:Emulsions; D005466:Fluorocarbons; D008074:Lipoproteins; D010743:Phospholipids; D002784:Cholesterol