title: High-performance liquid chromatographic method for the determination of alprazolam in plasma using the column-switching technique.
abstract: A reversed-phase high-performance liquid chromatographic method is described for the quantitative determination of alprazolam in the plasma of geriatric patients in the presence of 4-hydroxyalprazolam, alpha-hydroxyalprazolam, bromazepam, oxazepam, lorazepam, clobazam, desmethylclobazam, diazepam and desmethyldiazepam. The procedure is based on the enrichment of alprazolam on a PRP-1 pre-column, followed by the transfer of the compound in a forflush mode to the analytical column. Alprazolam can be quantified reliably down to a minimum concentration of 1 ng/ml of plasma.
authors.affiliation: Lehrstuhl für Innere Medizin, Gerontologie, Universität Erlangen-Nürnberg, Germany.
authors.forename: W
authors.initials: W
authors.lastname: Rieck
authors.forename: D
authors.initials: D
authors.lastname: Platt
mesh_terms: D000368:Aged; D000525:Alprazolam / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D011786:Quality Control; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet; D014229:Triazolam / Q000097:blood
chemical_list: D014229:Triazolam; D000525:Alprazolam