title: Effect of seizure activity and calpain inhibitor I on LTP in juvenile hippocampal slices.
abstract: Kainic acid-induced seizure activity in adult rats produces an impairment of long-term potentiation induction in hippocampal slices. As the consequences of seizure activity are different in adult and juvenile rats, we tested the ability of hippocampal slices prepared from kainate-treated juvenile rats to exhibit long-term potentiation. Long-term potentiation was induced by theta-burst stimulation and was not significantly different in slices prepared from control or kainate-injected juvenile rats (16-18 postnatal days). Short-term potentiation, however, was reduced in the kainate-treated juveniles. Calpain inhibitor I has been shown to prevent long-term potentiation formation in adult hippocampal slices, and we evaluated its effect on long-term potentiation in hippocampal slices from juvenile rats. Calpain inhibitor I produced a significant reduction in the degree of long-term potentiation induced by theta-burst stimulation in hippocampal slices prepared from 14-20 postnatal day-old animals. The results are consistent with the notion that, although similar mechanisms participate in the formation of long-term potentiation in juvenile and adult animals, juvenile animals are much more resistant than adult animals to the consequences of seizure activity.
authors.affiliation: Neuroscience Program, University of Southern California, Los Angeles 90089-2520.
authors.forename: J S
authors.initials: JS
authors.lastname: Fitzpatrick
authors.forename: K
authors.initials: K
authors.lastname: Shahi
authors.forename: M
authors.initials: M
authors.lastname: Baudry
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D006023:Glycoproteins / Q000494:pharmacology*; D006624:Hippocampus / Q000166:cytology* / Q000187:drug effects; D007051:Ibotenic Acid / Q000031:analogs & derivatives / Q000378:metabolism; D066298:In Vitro Techniques; D007608:Kainic Acid; D009473:Neuronal Plasticity / Q000187:drug effects*; D010741:Phospholipases A / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012640:Seizures / Q000139:chemically induced / Q000503:physiopathology*; D018350:alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid
chemical_list: D006023:Glycoproteins; C039304:calpain inhibitors; D007051:Ibotenic Acid; D018350:alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid; D010741:Phospholipases A; D007608:Kainic Acid