Replication of human immunodeficiency virus in HL-60 cells.
HL-60 cells carrying the CD4 marker could be productively infected with human immunodeficiency virus (HIV) but did not form syncytia, though 11-14 days p.i., there was a transient decrease in cell multiplication and viability. After prolonged passage, a subpopulation of HL-60 cells was selected. The virus produced differed from the initial input virus grown on CEM cells: the virions lacked knobs, were either empty or had abnormal cores, had a higher ratio p24/gp41,gp110 and were less infectious. After prolonged passage, virus was produced which was fully infectious for CEM but not for fresh HL-60 cells, and which ressembled the input virus with respect to morphology and p24/gp41,gp110 ratio.
Semmel
M
M
Unité 268 INSERM, Villejuif, France.
Macho
A
A
Morozov
V
V
Coulaud
D
D
Alileche
A
A
Plaisance
S
S
Aguilar
J
J
Jasmin
C
C
D000943:Antigens, Differentiation; D001483:Base Sequence; D015704:CD4 Antigens / Q000032:analysis*; D002460:Cell Line; D006678:HIV / Q000472:pathogenicity / Q000502:physiology* / Q000648:ultrastructure; D006801:Humans; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology / Q000382:microbiology*; D014764:Viral Proteins / Q000032:analysis*; D014771:Virion / Q000648:ultrastructure; D014779:Virus Replication
D000943:Antigens, Differentiation; D015704:CD4 Antigens; D014764:Viral Proteins; D012194:RNA-Directed DNA Polymerase