[PCR-SSCP--rapid and easy detection of DNA-sequence changes].
PCR-SSCP analysis is a rapid, simple and sensitive technique for detection of various mutations, including single nucleotide substitutions, insertions and deletions, in PCR-amplified DNA fragments. Here I review, the principle and sensitivity of the technique, and also show how this technique has been used in clinical and basic medical sciences.
Hayashi
K
K
Oncogene Division, National Cancer Center Research Institute, Tokyo, Japan.
D001483:Base Sequence; D004277:DNA, Single-Stranded / Q000235:genetics*; D006801:Humans; D009154:Mutation; D009369:Neoplasms / Q000175:diagnosis / Q000235:genetics; D009690:Nucleic Acid Conformation; D016133:Polymerase Chain Reaction / Q000379:methods*; D011110:Polymorphism, Genetic / Q000235:genetics*
D004277:DNA, Single-Stranded