title: Use of immobilized metal ions as a negative adsorbent for purification of enzymes: application to phosphoglycerate mutase from chicken muscle extract and horseradish peroxidase.
abstract: Two enzymes, phosphoglycerate mutase and peroxidase, were purified by using an immobilized metal ion adsorbent for the removal of unwanted proteins. The mutase was obtained pure from a single column, whereas the purification of peroxidase required the use of a thiophilic adsorbent in a tandem. The capacity was 2.5 mg pure peroxidase per mL gel.
authors.affiliation: Biochemical Separation Centre, Uppsala University, Sweden.
authors.forename: G
authors.initials: G
authors.lastname: Chaga
authors.forename: L
authors.initials: L
authors.lastname: Andersson
authors.forename: B
authors.initials: B
authors.lastname: Ersson
authors.forename: M
authors.initials: M
authors.lastname: Berg
mesh_terms: D000327:Adsorption; D000818:Animals; D001731:Bisphosphoglycerate Mutase / Q000302:isolation & purification*; D002412:Cations; D002645:Chickens; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D006735:Horseradish Peroxidase / Q000302:isolation & purification*; D007525:Isoelectric Focusing; D008670:Metals; D009132:Muscles / Q000201:enzymology*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D002412:Cations; D008670:Metals; D006735:Horseradish Peroxidase; D001731:Bisphosphoglycerate Mutase