Biopolymers from marine invertebrates. XII. A novel cytolytic factor from a hermit crab, Clibanarius longitarsus.
Bioactive polymers were sought in marine arthropoda and a novel cytolytic factor was found in a hermit crab, Clibanarius longitarsus. The partially purified factor showed activity in fractions corresponding to a molecular weight of about 10 kilodaltons on a Sephadex G-75 column. This cytolytic factor was halfmaximally active for tumor cells at 0.13-0.66 micrograms/ml and for normal cells at 1.9-82 micrograms/ml. Tumor lysis by the factor was time dependent and was complete within 12 h. This bioactive polymer was labile on heating, at low and high pH.
Kisugi
J
J
Faculty of Pharmaceutical Sciences, Teikyo University, Kanagawa, Japan.
Kamiya
H
H
Yamazaki
M
M
D000818:Animals; D033381:Anomura / Q000737:chemistry*; D000970:Antineoplastic Agents / Q000302:isolation & purification* / Q000494:pharmacology; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D051379:Mice; D008809:Mice, Inbred C3H
D000970:Antineoplastic Agents