Rapid effects of insulin on cyclic GMP location in an intact protozoan.
We studied rapid changes in location of cyclic GMP in Tetrahymena pyriformis. Insulin caused cGMP localization in cilia and near the plasma membrane (0.5-1 min). Later (1-5 min) cGMP localization was diffuse in cytoplasm with perinuclear accentuation. Inactive insulin analogs did not elicit these changes.
Kohidai
L
L
Metabolic Diseases Branch, NIDDK, Bethesda, Maryland 20892.
Barsony
J
J
Roth
J
J
Marx
S J
SJ
D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D002923:Cilia / Q000187:drug effects / Q000378:metabolism; D006152:Cyclic GMP / Q000378:metabolism*; D003593:Cytoplasm / Q000187:drug effects / Q000378:metabolism; D007328:Insulin / Q000031:analogs & derivatives / Q000494:pharmacology*; D009599:Nitroprusside / Q000494:pharmacology; D013769:Tetrahymena pyriformis / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D014018:Tissue Distribution
D007328:Insulin; D009599:Nitroprusside; D006152:Cyclic GMP; D015056:1-Methyl-3-isobutylxanthine