title: Microwave enhanced staining for plant virus inclusions.
abstract: Plant virus inclusion bodies can be stained specifically with established staining methods for light microscopy. The procedure can be augmented by a short microwave treatment to provide better staining intensity and reduced staining time. The method is useful for preliminary sampling prior to collection for electron microscopy and for plant pathologists, plant breeders, and diagnosticians as a rapid means of plant virus characterization.
authors.affiliation: USDA, Agricultural Research Station, Salinas, CA 93905.
authors.forename: L L
authors.initials: LL
authors.lastname: Hoefert
authors.forename: J D
authors.initials: JD
authors.lastname: McCreight
authors.forename: R D
authors.initials: RD
authors.lastname: Christie
mesh_terms: D001399:Azure Stains; D007181:Inclusion Bodies, Viral / Q000378:metabolism*; D008872:Microwaves; D010935:Plant Diseases / Q000382:microbiology*; D010942:Plant Viruses / Q000378:metabolism*; D013194:Staining and Labeling / Q000379:methods*; D013997:Time Factors; D014777:Virus Diseases / Q000175:diagnosis
chemical_list: D001399:Azure Stains; C025818:Azure A