Colony stimulating factor-1 stimulates Ishikawa cell proliferation and lipocortin II synthesis.
Proliferation of the Ishikawa human endometrial adenocarcinoma cell line is under the concerted control of oestrogen and progesterone. Here we demonstrate that Ishikawa cells express colony stimulating factor-1 (CSF-1), CSF-1 receptor mRNA and are growth stimulated by CSF-1 treatment. An early event associated with CSF-1 treatment is the induction of lipocortin II synthesis, a protein whose expression is also under oestrogen and progesterone control. However, neither CSF-1 or CSF-1 receptor mRNA appear to be modulated by oestrogen or progesterone.
Croxtall
J D
JD
Institute of Obstetrics and Gynaecology, Royal Postgraduate Medical School, Hammersmith Hospital, London, England.
Pollard
J W
JW
Carey
F
F
Forder
R A
RA
White
J O
JO
D000230:Adenocarcinoma / Q000378:metabolism* / Q000473:pathology; D017302:Annexins; D001483:Base Sequence; D002135:Calcium-Binding Proteins / Q000096:biosynthesis*; D002455:Cell Division / Q000187:drug effects*; D004591:Electrophoresis, Polyacrylamide Gel; D016889:Endometrial Neoplasms / Q000378:metabolism* / Q000473:pathology; D004967:Estrogens / Q000502:physiology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D016173:Macrophage Colony-Stimulating Factor / Q000494:pharmacology*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011233:Precipitin Tests; D011374:Progesterone / Q000502:physiology; D012333:RNA, Messenger / Q000096:biosynthesis; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D016186:Receptor, Macrophage Colony-Stimulating Factor / Q000235:genetics; D014407:Tumor Cells, Cultured
D017302:Annexins; D002135:Calcium-Binding Proteins; D004967:Estrogens; D012333:RNA, Messenger; D011374:Progesterone; D016173:Macrophage Colony-Stimulating Factor; D016186:Receptor, Macrophage Colony-Stimulating Factor; D012194:RNA-Directed DNA Polymerase