Biochemical and morphological differentiation of the human colonic epithelial cell line SW620 in the presence of dimethylsulfoxide.
In vitro models of intestinal cell differentiation provide an important adjunct for studying normal and abnormal intestinal epithelial cell differentiation. The studies reported herein describe morphologic and biochemical changes in the colonic epithelial cell line SW620 following dimethylsulfoxide (DMSO) incubation. Cells cultured in the presence of DMSO showed striking changes in morphology characterized by enlargement, elongation, and formation of process-like structures by light microscopy and a propensity to form microvillus-like structures by electron microscopy. These changes were accompanied by significant differences in the expression of the cell surface markers CD4 (HIV gp120 receptor), CD44 (hyaluronate receptor), and KS1 (adenocarcinoma/epithelial specific antigen). There was a marked decrease in CD4 expression (38% to 2%), an increase in CD44 expression (4% to 50%) and a decrease in KS1 expression (98% to 66%) as detected by flow cytometry following incubation of SW620 cells in DMSO. Parallel changes in the expression of these markers were seen by metabolic and surface labeling studies. Although SW620 cells were infected by HIV-1, DMSO-treated SW620 cells could not be infected. DMSO-induced changes in surface expression of CD4, CD44, and KS-1 were reversible over time upon removal of DMSO from the culture medium. Secretory component, sucrase, neuron-specific enolase, chromogranin-A, and mucin were not detectable in SW620 cells with or without DMSO treatment. SW620 cells provide a useful model for studying specific biochemical and molecular events involved in intestinal epithelial cell differentiation and function.
Omary
M B
MB
Department of Medicine, University of California San Diego, La Jolla 92093.
de Grandpre
L
L
McCaffrey
M
M
Kagnoff
M F
MF
D000951:Antigens, Neoplasm / Q000096:biosynthesis; D000954:Antigens, Surface / Q000096:biosynthesis; D015704:CD4 Antigens / Q000096:biosynthesis; D015815:Cell Adhesion Molecules; D002454:Cell Differentiation / Q000187:drug effects; D003106:Colon / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D004247:DNA / Q000096:biosynthesis; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D000071858:Epithelial Cell Adhesion Molecule; D004847:Epithelial Cells; D015497:HIV-1 / Q000502:physiology; D006801:Humans; D007700:Kinetics; D011233:Precipitin Tests; D014176:Protein Biosynthesis; D016201:Receptors, Lymphocyte Homing / Q000096:biosynthesis; D014407:Tumor Cells, Cultured
D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D015704:CD4 Antigens; D015815:Cell Adhesion Molecules; D000071858:Epithelial Cell Adhesion Molecule; D016201:Receptors, Lymphocyte Homing; D004247:DNA; D004121:Dimethyl Sulfoxide