title: Immune regulation by brain cells in the central nervous system: microglia but not astrocytes present myelin basic protein to encephalitogenic T cells under in vivo-mimicking conditions.
abstract: The antigen-presenting capability of various types of brain cell, such as primary mixed glial cells, astrocytes and microglia, was examined under conditions in which Ia antigen expression on the cultured cells mimicked that in the central nervous system (CNS) of rats with experimental autoimmune encephalomyelitis (EAE). In the CNS of rats with EAE, microglia but not astrocytes express Ia antigens. To produce such conditions, cultured brain cells were treated with various concentrations of interferon-gamma (IFN-gamma). It was revealed that in vivo-like conditions were produced when cultured brain cells were treated with less than 100 U/ml IFN-gamma. Under such conditions, microglia presented an antigen, myelin basic protein (MBP), to MBP-specific T-cell lines. Astrocytes, on the other hand, did not show antigen-presenting ability, but rather suppressed T-cell proliferation. Primary mixed glial cells, mainly comprising astrocytes and microglia, were also weak antigen-presenting cells (APC). These findings suggest that brain cells comprising various types of cell with regard to APC function do not up-regulate the proliferation of encephalitogenic T cells in vivo, although a particular type of brain cell, i.e. microglia, show antigen-presenting capability.
authors.affiliation: Department of Immunology, Niigata University School of Medicine, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Matsumoto
authors.forename: K
authors.initials: K
authors.lastname: Ohmori
authors.forename: M
authors.initials: M
authors.lastname: Fujiwara
mesh_terms: D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology*; D001253:Astrocytes / Q000276:immunology; D001327:Autoimmune Diseases / Q000276:immunology*; D002478:Cells, Cultured; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology*; D005260:Female; D005455:Fluorescent Antibody Technique; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D008297:Male; D004676:Myelin Basic Protein / Q000276:immunology*; D009457:Neuroglia / Q000276:immunology*; D051381:Rats; D011917:Rats, Inbred Lew
chemical_list: D000949:Histocompatibility Antigens Class II; D004676:Myelin Basic Protein