Increased membrane activity of glyceraldehyde 3-phosphate dehydrogenase in erythrocytes of patients with homozygous sickle cell anaemia.
Membrane-bound glyceraldehyde 3-phosphate dehydrogenase activity was measured in erythrocytes from 43 patients with sickle cell anaemia, 24 heterozygous and 27 controls. A significant increase of the activity was found among the patients but no such difference was observed between the heterozygous and the control individuals. The patients showed nearly non Gaussian distribution of the enzyme activity and were subgrouped on the basis of these results, subgroup I had normal values and subgroup II showed markedly increased activities. The patients in subgroup II had significantly lower blood haemoglobin concentrations and significantly higher lactate dehydrogenase activities in serum than subgroup I. The subgroups did not differ in blood reticulocyte counts, serum bilirubin, serum iron concentrations or band 3 protein content of erythrocyte membranes.
Mohamed
A O
AO
Department of Clinical Chemistry, University Hospital, Uppsala, Sweden.
Ronquist
G
G
al Bayoumi
R
R
D000293:Adolescent; D000755:Anemia, Sickle Cell / Q000201:enzymology* / Q000235:genetics; D002648:Child; D002675:Child, Preschool; D004910:Erythrocyte Membrane / Q000201:enzymology*; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000097:blood*; D006579:Heterozygote; D006720:Homozygote; D006801:Humans; D007223:Infant
D005987:Glyceraldehyde-3-Phosphate Dehydrogenases