An upstream activating sequence from the Aspergillus nidulans gpdA gene.
Introduction of a previously identified promoter element of the Aspergillus nidulans gpdA gene (encoding glyceraldehyde-3-phosphate dehydrogenase), the so-called gpd box, into the upstream region of the highly regulated A. nidulans amdS gene (encoding acetamidase), significantly increased (up to 30-fold) the expression of the lacZ reporter gene fused to these expression signals. This increase was dependent on the orientation of the gpd box and on the site of introduction into the amdS upstream region. The presence of additional gpdA sequences which flank the gpd box reduced or even extinguished positive effects of the gpd box. omega-Amino acid and carbon catabolite regulation of the amdS promoter were retained after introduction of the gpd box, indicating that the gpd box does not abolish interactions of the regulatory proteins, AmdR and CreA, with the amdS transcription control sequences. Based on the results, it is suggested that the gpd box comprises at least two separate activities: one being orientation dependent, but relatively independent of position of the gpd box in the upstream region, and the other is only functional near other sites of transcriptional control. Most likely, both activities are not involved in regulation of the amdS promoter.
Punt
P J
PJ
Department of Molecular Genetics and Gene-Technology, Medical Biological Laboratory TNO, Rijswijk, The Netherlands.
Kramer
C
C
Kuyvenhoven
A
A
Pouwels
P H
PH
van den Hondel
C A
CA
D001233:Aspergillus nidulans / Q000235:genetics*; D001483:Base Sequence; D015966:Gene Expression Regulation, Fungal; D005822:Genetic Vectors; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics
D005987:Glyceraldehyde-3-Phosphate Dehydrogenases