Effect of an antithyroid agent (propylthiouracil) and L-ascorbic acid on mixed-function oxidase and drug metabolism in hepatic microsomes of chickens.
1. The experiments were undertaken to determine if an antithyroid agent (propylthiouracil, PTU) and/or ascorbic acid (AA) affect mixed-function oxidase (MFO) in hepatic microsomes of male broiler chicks. 2. Feeding PTU increased the MFO activity in a dose-related manner. Addition of AA to the PTU-containing diet further increased the content of cytochromes P-450 and b5, but not the activities of NADPH-cytochrome c and NADH-cytochrome b5 reductase, and the drug-metabolizing enzymes. 3. Supplemental AA induced cytochrome b5 rather than cytochrome P-450.
Takahashi
K
K
Department of Animal Science, Faculty of Agriculture, Tohoku University, Sendai Japan.
Akiba
Y
Y
Horiguchi
M
M
D000818:Animals; D013956:Antithyroid Agents / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000494:pharmacology*; D002645:Chickens / Q000378:metabolism*; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D006899:Mixed Function Oxygenases / Q000187:drug effects*; D011441:Propylthiouracil / Q000494:pharmacology*
D013956:Antithyroid Agents; D011441:Propylthiouracil; D006899:Mixed Function Oxygenases; D001205:Ascorbic Acid