Assignment of a porcine male-specific DNA repeat to Y-chromosomal heterochromatin.
Primers were designed to amplify by PCR a 509-bp genomic fragment from male pig DNA, using the porcine male-specific repeat sequence described by McGraw et al. (1988). This PCR product showed male-specific hybridization in Southern blots. Nonradioactive in situ hybridization localized it to the entire length of the heterochromatic portion of Yq. The assignment was confirmed using the PCR primer pDYZ1-S for primed in situ labeling.
Thomsen
P D
PD
Department of Animal Science and Animal Health, Royal Veterinary and Agricultural University, Copenhagen, Denmark.
Hindkjaer
J
J
Christensen
K
K
D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D004247:DNA / Q000235:genetics*; D006570:Heterochromatin / Q000502:physiology* / Q000648:ultrastructure; D007621:Karyotyping; D008297:Male; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D016133:Polymerase Chain Reaction; D012091:Repetitive Sequences, Nucleic Acid; D013552:Swine / Q000235:genetics; D014998:Y Chromosome
D006570:Heterochromatin; D009838:Oligodeoxyribonucleotides; D004247:DNA