Radioprotection of mice by recombinant rat stem cell factor.
Treatment with recombinant rat stem cell factor (rSCF) protects mice from the lethal effects of irradiation. Mice treated with a single dose of rSCF prior to irradiation of up to 1150 rads [given as a split dose (1 rad = 0.01 Gy)] resulted in > 80% long-term survival, whereas a single injection given after the last dose of irradiation was not radioprotective. The combination of pre- and posttreatment (-20 h, -2 h, and +4 h) with rSCF resulted in 100% survival of otherwise lethally irradiated mice. Using this optimum schedule of rSCF administration, a radioprotective factor of 1.3-1.35 was achieved. The major cause of death in the control animals was massive bacteremia consisting of enteric organisms. The rSCF-treated animals had a much lower frequency of septicemia, due primarily to a rapid hematopoietic recovery of bone marrow function not evident in control animals.
Zsebo
K M
KM
Amgen, Inc., Thousand Oaks, CA 91320.
Smith
K A
KA
Hartley
C A
CA
Greenblatt
M
M
Cooke
K
K
Rich
W
W
McNiece
I K
IK
D000818:Animals; D001854:Bone Marrow Cells; D004334:Drug Administration Schedule; D006410:Hematopoiesis / Q000528:radiation effects; D016298:Hematopoietic Cell Growth Factors / Q000494:pharmacology*; D051379:Mice; D011837:Radiation-Protective Agents; D051381:Rats; D011994:Recombinant Proteins / Q000494:pharmacology; D018805:Sepsis / Q000517:prevention & control; D019089:Stem Cell Factor; D016019:Survival Analysis
D016298:Hematopoietic Cell Growth Factors; D011837:Radiation-Protective Agents; D011994:Recombinant Proteins; D019089:Stem Cell Factor