Stably inherited killer activity in industrial yeast strains obtained by electrotransformation.
Killer-sensitive strains of Saccharomyces cerevisiae and Saccharomyces carlsbergensis were transformed by electroinjection using double-stranded RNA isolated from a superkiller strain. Various recipient strains were used: both thermo-resistant and thermo-sensitive as well as mutants of industrial strains. Conversion of respiratory competent (rho+) into respiratory deficient (rho-) strains (mutants) resulted in a significant increase of the yield of electrotransformants and/or of longterm killer stability. Electrotransformation of rho- mutants of distillery and brewery strains resulted in more than 100 clones, which exhibited weak or strong killer activity over some or all of the experimental period of 10 months.
Salek
A
A
Lehrstuhl für Biotechnologie, Universität Würzburg, Biozentrum, FRG.
Schnettler
R
R
Zimmermann
U
U
D004272:DNA, Mitochondrial / Q000235:genetics; D004560:Electricity; D005285:Fermentation; D005800:Genes, Fungal; D055646:Killer Factors, Yeast; D009183:Mycotoxins / Q000235:genetics* / Q000276:immunology; D010641:Phenotype; D010957:Plasmids; D012330:RNA, Double-Stranded / Q000235:genetics; D012331:RNA, Fungal / Q000235:genetics; D012440:Saccharomyces / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins; D014170:Transformation, Genetic
D004272:DNA, Mitochondrial; D055646:Killer Factors, Yeast; D009183:Mycotoxins; D012330:RNA, Double-Stranded; D012331:RNA, Fungal; D029701:Saccharomyces cerevisiae Proteins