title: Ultrastructural analysis of platelet-like particles from a human megakaryocytic leukemia cell line (CMK 11-5).
abstract: Ultrastructural observations were performed to further characterize the human megakaryocytic leukemia cell line, CMK, and its subclone, CMK11-5. We found that particles derived from CMK11-5 cells had cytoplasmic projections and no nucleus, and that some particles contained alpha granules. Incubation with ADP induced fibrinogen receptors on the surface of these particles. Furthermore, the particles had glycoprotein Ib antigen on their surfaces, and attached nonreversibly to rabbit aortic subendothelium, showing associated morphological changes similar to those observed in normal platelets. CMK and CMK11-5 are the first megakaryocytic cell lines that have been found to release particles that have some of the same functions as normal platelets. In particular, CMK11-5 seems to be a useful model for studying megakaryocyte function.
authors.affiliation: First Department of Internal Medicine, Kansai Medical University, Osaka, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Nagano
authors.forename: S
authors.initials: S
authors.lastname: Ohga
authors.forename: Y
authors.initials: Y
authors.lastname: Kishimoto
authors.forename: T
authors.initials: T
authors.lastname: Kimura
authors.forename: K
authors.initials: K
authors.lastname: Yasunaga
authors.forename: M
authors.initials: M
authors.lastname: Adachi
authors.forename: R
authors.initials: R
authors.lastname: Ryo
authors.forename: T
authors.initials: T
authors.lastname: Sato
mesh_terms: D001792:Blood Platelets / Q000648:ultrastructure*; D006801:Humans; D013920:Thrombocythemia, Essential / Q000473:pathology*; D014407:Tumor Cells, Cultured