[Beta-mannosidase of trophoblast and humana skin fibroblasts].
Conditions for assay of beta-mannosidase activity in human chorionic villi were studied using the fluorogenic substrate 4-methylumbelliferyl-beta-D-mannopyranoside. Comparison of the biochemical properties of the chorionic villi beta-mannosidase with those of the enzyme from human cultured fibroblasts showed their similarity. Like the enzyme from skin fibroblasts, the chorionic villi beta-mannosidase had rather high activity. Both enzymes had virtually the same pH optimum (4.2-4.7) and Km value. The data presented suggest that chorion biopsy specimens can be used for prenatal determination of beta-mannosidase activity at the early stage of development.
Petushkov
N A
NA
Ivleva
T S
TS
D001706:Biopsy; D002478:Cells, Cultured; D002824:Chorionic Villi / Q000201:enzymology*; D005347:Fibroblasts / Q000201:enzymology*; D005470:Fluorometry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008361:Mannosidases / Q000032:analysis*; D011296:Prenatal Diagnosis; D012867:Skin / Q000201:enzymology*; D044902:beta-Mannosidase
D008361:Mannosidases; D044902:beta-Mannosidase