Liposome shrinkage and swelling under osmotic-diffusional stress: evaluation of kinetic parameters from spectrophotometric measurements.
Analyses of spectrophotometric measurements of shrinkage and swelling of liposomes under osmotic-diffusional stress tacitly assume an 'empirical' inverse relationship between the absorbance (A) and the liposomal volume (v). In this paper, we have proposed an alternate more explicit relationship: A = EcL e - alpha Lv/1-Lv where L is liposomal concentration, Ec the extinction coefficient and alpha a dimensionless parameter. The exponential term, in essence, defines the partitioning of aqueous volume into intra- and extra-liposomal compartments. Experimental data obtained with glycerol as model compound are used to test the validity and internal consistency of the proposed formalism.
Ghosh
P
P
Department of Biophysics, University of Delhi, India.
Singh
U N
UN
D007700:Kinetics; D008081:Liposomes / Q000737:chemistry*; D008433:Mathematics; D008954:Models, Biological; D008968:Molecular Conformation; D010713:Phosphatidylcholines / Q000737:chemistry*; D013053:Spectrophotometry; D013997:Time Factors
D008081:Liposomes; D010713:Phosphatidylcholines