title: cDNA encoding the chicken ortholog of the mouse dilute gene product. Sequence comparison reveals a myosin I subfamily with conserved C-terminal domains.
abstract: We report the cDNA-deduced primary structure of the chicken counterpart of the murine dilute gene product, a member of the myosin I family. Comparison of the chicken and mouse sequences reveals a distinct pattern of domains of high and low sequence conservation. An internal deletion of 25 amino acids probably reflects differential mRNA processing. Compared with other myosin heavy chain molecules, sequence similarity is highest with the MYO2 gene product of Saccharomyces cerevisiae. The MYO2 protein, implicated in vectorial vesicle transport, is homologous to the dilute protein over practically its entire length. In addition, the C-terminal domain of the dilute protein is highly similar to a putative glutamic acid decarboxylase sequence cloned from mouse brain. Alternatively, this closely related clone might represent an isoform of the dilute protein derived from a second gene, potentially involved in genetic conditions related to dilute.
authors.affiliation: Institut für Physiologische Chemie, Ruhr-Universität Bochum, Germany.
authors.forename: G
authors.initials: G
authors.lastname: Sanders
authors.forename: B
authors.initials: B
authors.lastname: Lichte
authors.forename: H E
authors.initials: HE
authors.lastname: Meyer
authors.forename: M W
authors.initials: MW
authors.lastname: Kilimann
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000502:physiology; D002352:Carrier Proteins / Q000235:genetics; D002645:Chickens / Q000235:genetics*; D005656:Fungal Proteins / Q000235:genetics; D015723:Gene Library; D005800:Genes, Fungal; D008099:Liver / Q000502:physiology; D051379:Mice; D008969:Molecular Sequence Data; D009124:Muscle Proteins / Q000235:genetics*; D009132:Muscles / Q000502:physiology; D018995:Myosin Heavy Chains; D015879:Myosin Subfragments / Q000235:genetics; D024462:Myosin Type II; D024701:Myosin Type V; D009218:Myosins / Q000235:genetics*; D011061:Poly A / Q000235:genetics / Q000302:isolation & purification; D016548:Prosencephalon / Q000502:physiology; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012333:RNA, Messenger; D012441:Saccharomyces cerevisiae / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins; D029702:Schizosaccharomyces pombe Proteins; D017386:Sequence Homology, Amino Acid
chemical_list: D002352:Carrier Proteins; D005656:Fungal Proteins; C077512:MYO2 protein, S cerevisiae; C434211:MYO2 protein, S pombe; D009124:Muscle Proteins; C513082:Myo5a protein, mouse; D015879:Myosin Subfragments; D012333:RNA, Messenger; D029701:Saccharomyces cerevisiae Proteins; D029702:Schizosaccharomyces pombe Proteins; D011061:Poly A; D012313:RNA; D024462:Myosin Type II; D024701:Myosin Type V; D018995:Myosin Heavy Chains; D009218:Myosins