Detection and identification of Yersinia pseudotuberculosis and pathogenic Yersinia enterocolitica by an improved polymerase chain reaction method.
We developed a polymerase chain reaction method in order to detect and identify both Yersinia pseudotuberculosis and pathogenic Yersinia enterocolitica. Polymerase chain reaction was performed by using a mixture of primers against the inv gene from Y. pseudotuberculosis and the ail gene from pathogenic Y. enterocolitica. Further addition of primers against the plasmid-coded virF gene from Y. enterocolitica made it possible to detect a virulence-associated gene of both species at the same time. This method was proved to be an adequate and convenient procedure for routine detection and identification of these bacilli.
Nakajima
H
H
Department of Microbiology, Okayama Prefectural Institute for Environmental Science and Public Health, Japan.
Inoue
M
M
Mori
T
T
Itoh
K
K
Arakawa
E
E
Watanabe
H
H
D000818:Animals; D015373:Bacterial Typing Techniques; D001483:Base Sequence; D003854:Deoxyribonucleotides; D004285:Dogs; D005798:Genes, Bacterial / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D009841:Oligonucleotides; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D014774:Virulence / Q000235:genetics; D015007:Yersinia / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015008:Yersinia enterocolitica / Q000145:classification / Q000302:isolation & purification* / Q000472:pathogenicity; D015011:Yersinia pseudotuberculosis / Q000145:classification / Q000302:isolation & purification*
D003854:Deoxyribonucleotides; D009841:Oligonucleotides