The synthesis of p58cyclin A and the phosphorylation of p34cdc2 are inhibited in human lymphoid cells arrested in G1 by alpha-interferon.
Daudi cells arrest in G1 in the presence of alpha-interferon. Such cells have little p58cyclin A, probably due to inhibition of p58cyclin A synthesis. The phosphorylation-associated migration shift of p34cdc2 is not seen in alpha-interferon-arrested cells. Cells arrested in late G1 by aphidicolin have abundant p58cyclin A and phosphorylated p34cdc2. Cell sorting showed that p58cyclin A increases in proliferating cells in late G1 and coincides with phosphorylation of p34cdc2.
Bybee
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A
University College and Middlesex School of Medicine, Department of Clinical Haematology, London, UK.
Thomas
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NS
D016590:Aphidicolin / Q000494:pharmacology; D015153:Blotting, Western; D016203:CDC2 Protein Kinase / Q000378:metabolism*; D002478:Cells, Cultured; D016213:Cyclins / Q000096:biosynthesis*; D004591:Electrophoresis, Polyacrylamide Gel; D016193:G1 Phase / Q000187:drug effects*; D006801:Humans; D016898:Interferon-alpha / Q000494:pharmacology*; D008221:Lymphoid Tissue / Q000166:cytology / Q000378:metabolism*; D010766:Phosphorylation; D011233:Precipitin Tests
D016213:Cyclins; D016898:Interferon-alpha; D016590:Aphidicolin; D016203:CDC2 Protein Kinase