title: [Study of possible biological transformation of kanamycin to amikacin].
abstract: For transformation of kanamycin A (Km) to amikacin (Ak) with acylating enzymes from B. circulans, a culture producing butirosin (Btn), cellular and acellular conversion systems and methods for chemical and biological identification of Km, Ak and Btn were developed. The level of conversion of Km to Ak in vivo and in vitro did not exceed 2 per cent.
authors.forename: Iu D
authors.initials: IuD
authors.lastname: Vikhanskiĭ
authors.forename: V R
authors.initials: VR
authors.lastname: Iarulin
authors.forename: Iu V
authors.initials: IuV
authors.lastname: Zhdanovich
authors.forename: Iu E
authors.initials: IuE
authors.lastname: Bartoshevich
authors.forename: V V
authors.initials: VV
authors.lastname: Esipova
authors.forename: L I
authors.initials: LI
authors.lastname: Nasonova
mesh_terms: D000215:Acylation; D000583:Amikacin / Q000378:metabolism*; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001407:Bacillus / Q000235:genetics / Q000378:metabolism*; D002076:Butirosin Sulfate / Q000096:biosynthesis; D003470:Culture Media; D006595:Hexosamines / Q000494:pharmacology; D066298:In Vitro Techniques; D007612:Kanamycin / Q000378:metabolism*; D009154:Mutation
chemical_list: D000900:Anti-Bacterial Agents; D003470:Culture Media; D006595:Hexosamines; D002076:Butirosin Sulfate; D007612:Kanamycin; D000583:Amikacin; C007265:2-deoxystreptamine