title: Evaluation of nonradioactive DNA probes for identification of mycobacteria.
abstract: Commercial chemiluminescent DNA probes (Accuprobe; Gen-Probe, San Diego, Calif.) for the identification of Mycobacterium tuberculosis (MTB) complex, M. avium complex (MAC), M. gordonae, and M. kansasii were evaluated with 134 clinical isolates. These included 36 MTB complex, 40 MAC, 27 M. gordonae, 9 M. kansasii, and 22 Mycobacterium spp. The specificity was 100% for the four probes. The sensitivity was 100% for the MTB complex and M. gordonae probes and 95.2% for the MAC probe. Five of the nine M. kansasii isolates tested were not detected with the probe.
authors.affiliation: Service de Microbiologie, Hôpital Antoine Béclère, Clamart, France.
authors.forename: L
authors.initials: L
authors.lastname: Lebrun
authors.forename: F
authors.initials: F
authors.lastname: Espinasse
authors.forename: J D
authors.initials: JD
authors.lastname: Poveda
authors.forename: V
authors.initials: V
authors.lastname: Vincent-Levy-Frebault
mesh_terms: D015342:DNA Probes; D005069:Evaluation Studies as Topic; D005188:False Negative Reactions; D008163:Luminescent Measurements; D009161:Mycobacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D012680:Sensitivity and Specificity
chemical_list: D015342:DNA Probes