Endogenous synthesis of N-methylsalsolinol, an analogue of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine, in rat brain during in vivo microdialysis with salsolinol, as demonstrated by gas chromatography-mass spectrometry.
N-Methylsalsolinol, an analogue of 1,2,3,6-tetrahydropyridine, is present in the brains of patients with Parkinson's disease. To determine the metabolic pathway for the synthesis of N-Methylsalsolinol in the brain, salsolinol was perfused through the striatum or the substantia nigra of the rat brain by in vivo microdialysis. N-Methylsalsolinol was detected in the brain dialysate samples during microdialysis with salsolinol using gas chromatography-mass spectrometry with selected-ion monitoring. These results demonstrate that endogenous N-methylation of salsolinol into N-methylsalsolinol occurs in the brain in vivo.
Niwa
T
T
Department of Internal Medicine, Nagoya University Branch Hospital, Japan.
Maruyama
W
W
Nakahara
D
D
Takeda
N
N
Yoshizumi
H
H
Tatematsu
A
A
Takahashi
A
A
Dostert
P
P
Naoi
M
M
Nagatsu
T
T
D015632:1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine / Q000031:analogs & derivatives*; D000818:Animals; D001921:Brain / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D007546:Isoquinolines / Q000008:administration & dosage* / Q000493:pharmacokinetics; D008297:Male; D008745:Methylation; D010300:Parkinson Disease / Q000378:metabolism; D010477:Perfusion; D051381:Rats; D017208:Rats, Wistar; D012490:Salsoline Alkaloids / Q000378:metabolism*; D044005:Tetrahydroisoquinolines
D007546:Isoquinolines; D012490:Salsoline Alkaloids; D044005:Tetrahydroisoquinolines; C036617:salsolinol; D015632:1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine; C032837:salsoline