title: Analysis of inter-serotypic structural relationships of infectious bursal disease virus using detergent solubilization and radioimmunoprecipitation with monoclonal antibodies.
abstract: Monoclonal antibodies (MAbs) neutralizing only the infectious bursal disease virus strains (IBDV) belonging to serotype 1 also immunoprecipitated the heterologous major antigenic proteins of serotype 2 IBDV. Detergent-solubilization followed by radioimmunoprecipitation assays (RIPA) using the MAbs revealed structural similarities between the conformation-dependent antigenic determinants of IBDV of the two existing serotypes. The presence of non-ionic detergent Triton X-100 determined the binding of altered proteins by MAbs in RIPA.
authors.affiliation: Section Virologie, Faculté de médecine vétérinaire, Université de Montréal, St-Hyacinthe, Québec, Canada.
authors.forename: S K
authors.initials: SK
authors.lastname: Reddy
authors.forename: A
authors.initials: A
authors.lastname: Silim
authors.forename: D
authors.initials: D
authors.lastname: Frenette
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D003902:Detergents; D000939:Epitopes / Q000737:chemistry; D007243:Infectious bursal disease virus / Q000145:classification*; D015531:Radioimmunoprecipitation Assay; D012703:Serotyping; D012995:Solubility; D014709:Vero Cells
chemical_list: D000911:Antibodies, Monoclonal; D003902:Detergents; D000939:Epitopes