The effect of day of the estrous cycle, location of ovulatory structure, and progesterone on in vitro bovine endometrial secretions.
Endometrial tissue was collected by biopsy from mature Holstein cows either on d 0 (estrus) or on d 9, 14, or 18 of the estrous cycle to determine the effects of day of the cycle, uterine horn, and in vitro progesterone on endometrial protein secretion. Tissue was incubated for 24 h in supplemented media containing 14C amino acids and either 0, 4.7, or 47 ng of progesterone/ml. Media were analyzed for total protein, radiolabeled protein, and profile of protein released during incubation. Endometrial tissue at d 0 released more protein than did tissue collected on all other days. Radiolabeled proteins were greater on d 0 and 18 than on d 9 and 14. Endometrium from the uterine horn contralateral to the corpus luteum released more radiolabeled protein than endometrium from the uterine horn ipsilateral to the corpus luteum. Seventeen protein bands were identified by electrophoresis. Proximity of the uterine horn to the site of ovulation affected the distribution of specific bands 21,400, 55,000, 74,600, and 88,100 molecular weight. The proportion of released proteins represented by proteins of molecular weights 12,700, 19,100, 21,400, 32,000, and 66,500 was affected either by day of the estrous cycle, proximity of uterine horn to the site of ovulation, or progesterone concentration. The results show that day of the estrous cycle and uterine horn not only alter overall endometrial protein secretion and synthetic activity but also have specific effects on distribution of individual proteins.
Williams
B L
BL
Department of Dairy Science, College of Agriculture and Life Sciences, Virginia Polytechnic Institute and State University, Blacksburg 24061-0315.
Gwazdauskas
F C
FC
Pearson
R E
RE
D000818:Animals; D002417:Cattle / Q000502:physiology*; D004591:Electrophoresis, Polyacrylamide Gel; D004717:Endometrium / Q000187:drug effects / Q000378:metabolism*; D004971:Estrus / Q000502:physiology*; D005260:Female; D066298:In Vitro Techniques; D008970:Molecular Weight; D010060:Ovulation; D011374:Progesterone / Q000494:pharmacology*; D011506:Proteins / Q000737:chemistry / Q000378:metabolism*
D011506:Proteins; D011374:Progesterone