Histochemistry of the porcine pilosebaceous unit.
The present study describes lectin and immunoreactivity in the pilosebaceous unit of porcine skin. Complex carbohydrates of mucin and biantennary Man/Gluc types were distributed among hair follicle epithelia (hair root sheaths, cuticula, shaft, and shaft matrix). Sebaceous glands expressed biantennary Man/Gluc carbohydrates and GalNAc residues. The expression of simple-type and epidermis-like keratins was confirmed by immunohistochemistry with monoclonal antibodies. Filaggrin-positive cells were found in the keratinizing zone of Henle's layer in anagen follicles. The innermost layer of the outer hair root sheath was stained with antibodies against the epidermal growth factor-receptor, keratin 10 and Ki67 antigen. The differences to humans were remarkably small.
Wollina
U
U
Department of Dermatology, Friedrich-Schiller-University, Jena, Germany.
Berger
U
U
Stolle
C
C
Stolle
H
H
Schubert
H
H
Hipler
C
C
D000818:Animals; D000911:Antibodies, Monoclonal; D001665:Binding Sites; D002147:Calmodulin / Q000032:analysis; D002241:Carbohydrates / Q000032:analysis*; D000091344:Filaggrin Proteins; D006197:Hair / Q000737:chemistry*; D006801:Humans; D007381:Intermediate Filament Proteins / Q000032:analysis; D007633:Keratins / Q000032:analysis; D037102:Lectins / Q000378:metabolism; D009077:Mucins / Q000032:analysis; D011506:Proteins / Q000032:analysis*; D012627:Sebaceous Glands / Q000737:chemistry*; D012867:Skin / Q000737:chemistry; D013194:Staining and Labeling; D013552:Swine / Q000378:metabolism*; D013556:Swine, Miniature / Q000378:metabolism
D000911:Antibodies, Monoclonal; D002147:Calmodulin; D002241:Carbohydrates; C000717249:FLG protein, human; D000091344:Filaggrin Proteins; D007381:Intermediate Filament Proteins; D037102:Lectins; D009077:Mucins; D011506:Proteins; D007633:Keratins