Identification of the hepatocyte mitogen in bovine spleen as heparin-binding growth factors.
Growth promoting activity for rat hepatocytes in bovine spleen was identified as three heparin-binding growth factors. All the features tested, such as heparin affinity, molecular mass, cross reactivity with antibody, and partial amino acid sequence, indicated that one of the three factors was identical to FGF-1 (fibroblast growth factor-1, acidic FGF), another one was related to FGF-2 (fibroblast growth factor-2, basic FGF), whereas it was more potent for hepatocytes than the FGF-2 purified from bovine brain. The third one was eluted from heparin-Sepharose column at 0.75M NaCl, of which activity was not abolished by anti-FGF-1 or FGF-2 antibodies. In addition, the mitogenic effect of this factor was synergistic with that of HGF (hepatocyte growth factor), a known potent hepatocyte mitogen, suggesting that it is a novel growth factor for hepatocytes.
Suzuki
M
M
Cell Science and Technology Division, Fermentation Research Institute, Ibaraki, Japan.
Itoh
T
T
Suzuki
T
T
Koga
N
N
Kato
K
K
Saito
T
T
Mitsui
Y
Y
D016475:3T3 Cells; D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002846:Chromatography, Affinity; D004261:DNA Replication / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D016220:Fibroblast Growth Factor 1 / Q000302:isolation & purification* / Q000494:pharmacology*; D016222:Fibroblast Growth Factor 2 / Q000302:isolation & purification* / Q000494:pharmacology*; D007700:Kinetics; D008099:Liver / Q000166:cytology* / Q000187:drug effects; D051379:Mice; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000302:isolation & purification; D051381:Rats; D011919:Rats, Inbred Strains; D013154:Spleen / Q000737:chemistry*; D013936:Thymidine / Q000378:metabolism; D014316:Tritium
D010446:Peptide Fragments; D014316:Tritium; D016222:Fibroblast Growth Factor 2; D016220:Fibroblast Growth Factor 1; D013936:Thymidine