"The effect of hCG-induced desensitization on RNA synthesis in rat Leydig cells".
In this study we have examined the effect of hCG-induced desensitization on RNA synthesis in rat Leydig cells. In vitro [3H]-uridine incorporation into RNA decreased after a high, single dose of hCG (100 IU). This effect was maximal after the second and third day of treatment. When Leydig cells were incubated in vitro for 30 min or more, a marked decrease of total and poly(A)+ RNA synthesis was observed. This was not due to reduced cell permeability to the radioactive nucleotide, indicating a truly decreased RNA synthesis during desensitization. The magnitude of this inhibitory effect (73-80%) suggests that desensitization may involve other biological functions of Leydig cell.
Ronco
A M
AM
Unidad de Biología de la Reproducción, Universidad de Chile, Santiago.
Valladares
L
L
D000818:Animals; D006063:Chorionic Gonadotropin / Q000502:physiology*; D007700:Kinetics; D007985:Leydig Cells / Q000378:metabolism*; D008297:Male; D011061:Poly A / Q000096:biosynthesis; D012313:RNA / Q000096:biosynthesis*; D051381:Rats; D011919:Rats, Inbred Strains; D013739:Testosterone / Q000096:biosynthesis
D006063:Chorionic Gonadotropin; D011061:Poly A; D013739:Testosterone; D012313:RNA