1H-NMR spectroscopy of serum in SHN mice with and without spontaneous mammary tumours.
Proton nuclear magnetic resonance (1H-NMR) spectra of serum were studied before and after the appearance of spontaneous mammary tumours in intact and anterior pituitary grafted SHN mice. In the sera of non-tumourous animals, the lipid CH2 and CH3 resonances at 1.31 and 0.88 ppm, possibly the peaks of very low density lipoprotein (VLDL), were unclear; however, such resonances were apparently observed in the sera of mammary tumour bearing mice. On the contrary, alanine, beta-hydroxybutyrate, valine and leucine, which peaked clearly in non-tumourous mice, declined greatly in tumourous mice. Livers of tumourous mice were heavier than those of non-tumourous animals. Quite similar trends in serum spectra and liver weight were observed in mice grafted with two anterior pituitaries. These findings indicate that tumour absorption of VLDL increases and other metabolic pathways are largely altered in association with mammary tumour progression. Prolactin, which is essential for mammary tumour development, had little effect on serum spectra of mice either with or without mammary tumours.
Fujii
Y
Y
Experimental Animal Research Laboratory, Meiji University, Kanagawa, Japan.
Nishiyama
H
H
Nagasawa
H
H
D020155:3-Hydroxybutyric Acid; D000409:Alanine / Q000097:blood; D000818:Animals; D005260:Female; D006885:Hydroxybutyrates / Q000097:blood; D007930:Leucine / Q000097:blood; D008079:Lipoproteins, VLDL / Q000378:metabolism*; D008099:Liver / Q000473:pathology; D009682:Magnetic Resonance Spectroscopy; D015674:Mammary Neoplasms, Animal / Q000097:blood* / Q000473:pathology; D051379:Mice; D008815:Mice, Inbred Strains; D009929:Organ Size; D014633:Valine / Q000097:blood
D006885:Hydroxybutyrates; D008079:Lipoproteins, VLDL; D007930:Leucine; D014633:Valine; D000409:Alanine; D020155:3-Hydroxybutyric Acid