title: Pulsed electric current enhances the phorbol ester induced oxidative burst in human neutrophils.
abstract: Oxidative burst (OB) response in human neutrophils, measured with chemiluminescence (CL), has been used to determine whether pulsed electric current (PEC) might induce a functional response in these electrically nonexcitable cells, and also whether it might modify cellular response to tumor-promoting phorbol ester (PMA). Five minutes of PEC treatment caused no significant changes in neutrophil CL levels in HBSS (1.2 mM Ca2+ concentration) as well as in HBSS-EGTA, where the extracellular Ca2+ concentration was reduced to less than 30 nM. The CL level of PMA-activated neutrophils in HBSS was 52% higher than in HBSS-EGTA. In HBSS the CL level, after the combined PMA and PEC treatment, was 53% higher than in PMA-alone-treated neutrophils. Activation of the OB in HBSS-EGTA with PMA and PEC was 13% higher than in solely PMA treated neutrophils. The results suggest that in neutrophil OB response, the PEC effect is closely related with cellular calcium mobilization, since depletion of extracellular Ca2+ decreased the PEC effect.
authors.affiliation: Faculty of Electrical and Computer Engineering, University of Ljubljana, Slovenia.
authors.forename: F
authors.initials: F
authors.lastname: Bobanović
authors.forename: S
authors.initials: S
authors.lastname: Simcic
authors.forename: V
authors.initials: V
authors.lastname: Kotnik
authors.forename: L
authors.initials: L
authors.lastname: Vodovnik
mesh_terms: D000328:Adult; D004558:Electric Stimulation; D006801:Humans; D008163:Luminescent Measurements; D008875:Middle Aged; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism*; D016897:Respiratory Burst / Q000187:drug effects*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
chemical_list: D013755:Tetradecanoylphorbol Acetate