title: [An effective concentration method for human immunodeficiency virus type 1 (HIV-1)].
abstract: We designed an effective virus concentration method to prevent the infection with human immunodeficiency virus type 1 (HIV-1) in laboratories. The absorbent of Minicon concentrators (Amicon Division, M.R. Grace & Co.-Conn.) was changed to chitin, a mucopolysaccharide extracted from the shells of Japanese pink crab. HIV-1 in the supernatant of HIV-1 infected Molt-4 cells was concentrated by Minicon and the new concentrators. The new concentrator showed good concentration rate and equality of concentration speed.
authors.affiliation: Department of Bacteriology, Kinki University School of Medicine.
authors.forename: T
authors.initials: T
authors.lastname: Takasaki
authors.forename: K
authors.initials: K
authors.lastname: Sano
authors.forename: E
authors.initials: E
authors.lastname: Tanaka
authors.forename: S
authors.initials: S
authors.lastname: Morimatsu
authors.forename: K
authors.initials: K
authors.lastname: Kifune
authors.forename: R
authors.initials: R
authors.lastname: Tsurutani
authors.forename: M
authors.initials: M
authors.lastname: Nakai
authors.forename: J
authors.initials: J
authors.lastname: Yamaguchi
mesh_terms: D002686:Chitin / Q000737:chemistry; D015497:HIV-1 / Q000302:isolation & purification*; D008828:Microbiological Techniques
chemical_list: D002686:Chitin