title: Bacillus methanolicus sp. nov., a new species of thermotolerant, methanol-utilizing, endospore-forming bacteria.
abstract: The generic position of 14 strains of gram-positive bacteria able to use methanol as a growth substrate was determined. All are obligately aerobic, thermotolerant organisms that are able to grow at temperatures of 35 to 60 degrees C. Nine of the strains produce oval spores at a subterminal-to-central position in slightly swollen rod-shaped cells. DNA-DNA hybridization studies, 5S rRNA sequence analysis, and physiological characteristics revealed that all 14 strains cluster as a well-defined group and form a distinct new genospecies. Analysis of the 16S and 5S rRNA sequences indicated that this new species is distinct from Bacillus brevis but closely related to B. firmus and B. azotoformans. The name proposed for this new species is B. methanolicus. The type strain, PB1, has been deposited in the National Collection of Industrial and Marine Bacteria as NCIMB 13113.
authors.affiliation: Department of Microbiology, University of Groningen, Haren, The Netherlands.
authors.forename: N
authors.initials: N
authors.lastname: Arfman
authors.forename: L
authors.initials: L
authors.lastname: Dijkhuizen
authors.forename: G
authors.initials: G
authors.lastname: Kirchhof
authors.forename: W
authors.initials: W
authors.lastname: Ludwig
authors.forename: K H
authors.initials: KH
authors.lastname: Schleifer
authors.forename: E S
authors.initials: ES
authors.lastname: Bulygina
authors.forename: K M
authors.initials: KM
authors.lastname: Chumakov
authors.forename: N I
authors.initials: NI
authors.lastname: Govorukhina
authors.forename: Y A
authors.initials: YA
authors.lastname: Trotsenko
authors.forename: D
authors.initials: D
authors.lastname: White
mesh_terms: D001407:Bacillus / Q000145:classification* / Q000502:physiology; D001482:Base Composition; D001483:Base Sequence; D004269:DNA, Bacterial / Q000737:chemistry; D000432:Methanol / Q000378:metabolism*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012329:RNA, Bacterial / Q000737:chemistry; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012341:RNA, Ribosomal, 5S / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D013171:Spores, Bacterial / Q000502:physiology
chemical_list: D004269:DNA, Bacterial; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012341:RNA, Ribosomal, 5S; D000432:Methanol