title: Cytogenetic study in peripheral blood lymphocytes from asthmatic patients receiving continued therapy with theophylline.
abstract: Possible cytogenetic effects of theophylline have been investigated in asthmatic patients undergoing continuous therapy with this drug. Sister-chromatid exchanges (SCE), chromosome aberrations (CA) and proliferating rate indices (PRI) were evaluated in cultured peripheral blood lymphocytes from patients receiving theophylline alone, theophylline plus inhaled beta 2 adrenergic drugs or theophylline in combination with beta 2 adrenergic agents and corticoids. Two samples from each individual were obtained in order to perform a prospective study: before the theophylline medication (sample A) and at a time after the beginning of treatment (sample B). After treatment (66.3 +/- 37.8 days), an increase in SCE was observed without modifications either in PRI or in CA. Patients receiving beta 2 adrenergics or beta 2 adrenergics plus glucocorticoids before and during theophylline treatment, did not respond differently than those on theophylline alone.
authors.affiliation: Department of Pharmacology, Medical School, University of Zaragoza, Spain.
authors.forename: B
authors.initials: B
authors.lastname: Sinues
authors.forename: A
authors.initials: A
authors.lastname: Broto
authors.forename: M A
authors.initials: MA
authors.lastname: Suarez
authors.forename: F
authors.initials: F
authors.lastname: Duce
authors.forename: A
authors.initials: A
authors.lastname: Martinez-Berganza
authors.forename: M L
authors.initials: ML
authors.lastname: Bernal
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D001249:Asthma / Q000097:blood* / Q000188:drug therapy; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D004359:Drug Therapy, Combination; D005260:Female; D005938:Glucocorticoids / Q000627:therapeutic use; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009153:Mutagens / Q000633:toxicity*; D051381:Rats; D012854:Sister Chromatid Exchange / Q000187:drug effects; D013806:Theophylline / Q000009:adverse effects* / Q000627:therapeutic use
chemical_list: D005938:Glucocorticoids; D009153:Mutagens; D013806:Theophylline