Endogenous cholecystokinin, the major factor responsible for dietary protein-induced pancreatic growth.
This study was undertaken to clarify the role of endogenous cholecystokinin (CCK) in induction of pancreatic growth stimulated by a high protein diet. Rats with i.v. jugular cannulae in place and kept in Bollman cages were adapted to 5% casein diet for 9 days and switched to 70% casein for 2 days. MK-329, a CCK receptor antagonist, and SMS 201-995, a somatostatin agonist, were continuously infused at 0.5 mg/kg/h and 5 micrograms/kg/h, respectively, starting at the onset of feeding 70% casein. The 5 and 70% casein control groups were infused with saline. Feeding 70% casein significantly stimulated pancreatic hyperplasia and tissue hypertrophy. MK-329 and SMS 201-995 totally prevented 70% casein-induced increases in pancreatic weight and total RNA and DNA contents. The results indicate that endogenous CCK is the major factor responsible for pancreatic growth induced by a high protein diet.
Morisset
J
J
Département de Biologie, Faculté des Sciences, Université de Sherbrooke, Québec, Canada.
Guan
D
D
Jurkowska
G
G
Rivard
N
N
Green
G M
GM
D000681:Amylases / Q000378:metabolism; D000818:Animals; D001570:Benzodiazepinones / Q000494:pharmacology; D001835:Body Weight; D002364:Caseins / Q000008:administration & dosage / Q000494:pharmacology; D002766:Cholecystokinin / Q000502:physiology*; D002919:Chymotrypsinogen / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D020109:Devazepide; D004044:Dietary Proteins / Q000494:pharmacology*; D006965:Hyperplasia; D015282:Octreotide / Q000494:pharmacology; D009929:Organ Size; D010179:Pancreas / Q000187:drug effects / Q000473:pathology*; D011506:Proteins / Q000378:metabolism; D012313:RNA / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D011949:Receptors, Cholecystokinin / Q000037:antagonists & inhibitors
D001570:Benzodiazepinones; D002364:Caseins; D004044:Dietary Proteins; D011506:Proteins; D011949:Receptors, Cholecystokinin; D012313:RNA; D004247:DNA; D002766:Cholecystokinin; D002919:Chymotrypsinogen; D000681:Amylases; D020109:Devazepide; D015282:Octreotide