Severe in vitro inhibition of erythropoiesis and transient stimulation of granulopoiesis after bone-marrow infection with eight different HIV-2 isolates.
OBJECTIVE
To correlate severe anaemia and frequent neutropenia in HIV-2-infected patients with an inhibitory effect on bone-marrow progenitors common to several HIV-2 isolates.


METHODS
The effects of eight HIV-2 isolates on early (BFU-E) and late (CFU-E) erythroid progenitors and on granulomonocytic (CFU-GM) progenitors, produced in long-term bone-marrow cultures (LTBMC), were studied.


METHODS
Absolute numbers of BFU-E, CFU-E and CFU-GM per culture flask were calculated weekly for each HIV-2-infected LTBMC using semi-solid clonogenic assays, and compared with those obtained in mock-infected LTBMC. Levels of significance for comparisons were determined by an analysis of variance (ANOVA).


RESULTS
Pooled data from 24 series of LTBMC (three series for each HIV-2 isolate) revealed 80 and 100% inhibition of BFU-E and CFU-E on days 6 and 12 of LTBMC, respectively, while transient stimulation of CFU-GM was observed between days 14 and 20 of LTBMC, followed by total inhibition on day 30.


CONCLUSIONS
These results confirm a direct inhibitory effect of HIV-2 on in vitro haematopoiesis. The similar pattern of erythroid progenitor inhibition obtained from seven out of eight isolates suggests that the inhibitory effect on erythropoiesis is a feature common to a large number of HIV-2 isolates, which correlates with clinical findings.
Calenda
V
V
INSERM Unité 322, Unité de Recherches sur les Rétrovirus et Maladies Associées, Campus Universitaire de Luminy, Marseille, France.
Chermann
J C
JC
D015139:Blotting, Southern; D001853:Bone Marrow / Q000382:microbiology*; D001854:Bone Marrow Cells; D004279:DNA, Viral / Q000032:analysis; D015672:Erythroid Precursor Cells / Q000166:cytology / Q000382:microbiology; D004920:Erythropoiesis; D006098:Granulocytes / Q000166:cytology / Q000382:microbiology*; D015498:HIV-2 / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D006801:Humans; D066298:In Vitro Techniques; D016133:Polymerase Chain Reaction; D011533:Proviruses / Q000235:genetics / Q000302:isolation & purification; D014779:Virus Replication
D004279:DNA, Viral