Anaesthetic doses of pentobarbital antagonize phosphatidylinositol hydrolysis induced by substance P or carbachol in the spinal cord and cerebral cortex of the rat.
Basal as well as agonist (100 microM substance P or 2 mM carbachol)-stimulated phosphatidylinositol hydrolysis was investigated in slices of rat spinal cord and cerebral cortex, in the presence and absence of pentobarbital (5-50 microM in vitro or 65 mg/kg in vivo) or urethane (0.2 mM in vitro or 1.4 g/kg in vivo). Urethane was without effect; pentobarbital, however, inhibited the basal hydrolysis and the agonist-stimulated phosphatidylinositol hydrolysis (in dose-dependent fashion in vitro). It is suggested that inhibition of phosphatidylinositol hydrolysis may be part of the cellular mechanisms by which pentobarbital produces anaesthesia.
Hasséssian
H
H
Department of Physiology, Faculty of Medicine, Université de Montréal, Québec, Canada.
Prat
A
A
Couture
R
R
D000758:Anesthesia; D000818:Animals; D002217:Carbachol / Q000494:pharmacology*; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism; D006868:Hydrolysis / Q000187:drug effects; D066298:In Vitro Techniques; D010424:Pentobarbital / Q000494:pharmacology*; D010716:Phosphatidylinositols / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D013116:Spinal Cord / Q000187:drug effects / Q000378:metabolism; D013373:Substance P / Q000494:pharmacology*
D010716:Phosphatidylinositols; D013373:Substance P; D002217:Carbachol; D010424:Pentobarbital