A quantitative method for measuring the adherence of group B streptococci to murine peritoneal exudate macrophages.
We have developed a solid phase, direct binding, enzyme-linked immunosorbent assay (ELISA) to detect and quantify the adherence of group B streptococci to murine macrophages. The assay correlated well with direct microscopic quantification of adherence. As few as 3.8 x 10(4) bacteria/assay well or less than one bacterium per macrophage could be detected. This assay is both quantitative and selective, and is readily adaptable for multiple sample analysis. It provides a valuable alternative to visual detection of bacterial adherence.
Sloan
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Department of Microbiology, University of New Hampshire, Durham 03824-3544.
Pistole
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D000818:Animals; D001422:Bacterial Adhesion; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D008264:Macrophages / Q000382:microbiology* / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C; D010587:Phagocytosis; D013292:Streptococcus agalactiae / Q000472:pathogenicity*