title: Ordering of six polymorphic DNA markers important in the delineation of 3p deletions in neoplasia.
abstract: Using fluorescence in situ hybridisation (FISH) the chromosomal location and relative order of six human chromosome 3 probes has been determined. The sensitivity of the technique has enabled the relative mapping of probes carrying inserts as small as 500 basepairs (bp), thus allowing the following proximal-distal probe order to be proposed: D3S30 (3p13-14), D3S4 (3p13-14), D3S2 (distal 3p14), D3S32 (3p21), D3S48E (3p21-23), and D3S11 (3p22-23). These data combined with the deletion mapping data of other researchers raise the possibility that the loss of more than one region of the short arm of chromosome 3 may be important in the development of small cell lung cancer.
authors.affiliation: MRC Clinical Oncology and Radiotherapeutics Unit, Cambridge, United Kingdom.
authors.forename: A C
authors.initials: AC
authors.lastname: Heppell-Parton
authors.forename: D G
authors.initials: DG
authors.lastname: Albertson
authors.forename: P H
authors.initials: PH
authors.lastname: Rabbitts
mesh_terms: D018288:Carcinoma, Small Cell / Q000235:genetics*; D002871:Chromosome Banding; D002872:Chromosome Deletion; D002874:Chromosome Mapping; D002893:Chromosomes, Human, Pair 3; D015342:DNA Probes; D005819:Genetic Markers; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics*; D008856:Microscopy, Fluorescence; D009693:Nucleic Acid Hybridization
chemical_list: D015342:DNA Probes; D005819:Genetic Markers