Expression of the bcl-2 gene product in follicular lymphoma.
Expression of bcl-2 protein was analyzed in 140 cases of follicular lymphoma by immunohistologic staining of paraffin-embedded tissue; 85% of cases were positive, the frequency being related to histologic grade (100% for the small-cleaved cell type, 86% for the mixed cell type, and 76% for the large cell group). There was striking heterogeneity of bcl-2 content in a number of cases and the smaller neoplastic cells (i.e., centrocytes) were usually the most strongly labeled. In most cases, bcl-2 protein staining was much weaker in normal lymphoid cells than in the neoplastic cells. In several cases, staining for bcl-2 revealed patterns of neoplastic cell spread into adjacent tissue (e.g., normal follicles, lymphoid sinuses), and bcl-2 protein expression tended to be highest in these migratory cells.
Gaulard
P
P
Department of Pathology, Henri Mondor Hospital, Créteil, France.
d'Agay
M F
MF
Peuchmaur
M
M
Brousse
N
N
Gisselbrecht
C
C
Solal-Celigny
P
P
Diebold
J
J
Mason
D Y
DY
D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D008224:Lymphoma, Follicular / Q000378:metabolism* / Q000473:pathology; D009361:Neoplasm Invasiveness; D011518:Proto-Oncogene Proteins / Q000378:metabolism*; D019253:Proto-Oncogene Proteins c-bcl-2; D013194:Staining and Labeling
D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2