Nucleotide and deduced amino acid sequences of the lacR, lacABCD, and lacFE genes encoding the repressor, tagatose 6-phosphate gene cluster, and sugar-specific phosphotransferase system components of the lactose operon of Streptococcus mutans.
The complete nucleotide sequences of lacRABCDF and partial nucleotide sequence of lacE from the lactose operon of Streptococcus mutans are presented. Comparison of the streptococcal lac determinants with those of Staphylococcus aureus and Lactococcus lactis indicate exceptional protein and nucleotide identity. The deduced polypeptides also demonstrate significant, but lower, sequence similarity with the corresponding lactose proteins of Lactobacillus casei. Additionally, LacR has sequence homology with the repressor (DeoR) of the Escherichia coli deoxyribonucleotide operon, while LacC is similar to phosphokinases (FruK and PfkB) from E. coli. The primary translation products of the lacRABCDFE genes are polypeptides of 251 (M(r) 28,713), 142 (M(r) 15,610), 171 (M(r) 18,950), 310 (M(r) 33,368), 325 (M(r) 36,495), 104 (M(r) 11,401), and 123 (NH2-terminal) amino acids, respectively. As inferred from their direct homology to the staphylococcal lac genes, these determinants would encode the repressor of the streptococcal lactose operon (LacR), galactose-6-phosphate isomerase (LacA and LacB), tagatose-6-phosphate kinase (LacC), tagatose-1,6-bisphosphate aldolase (LacD), and the sugar-specific components enzyme III-lactose (LacF) and enzyme II-lactose (LacE) of the S. mutans phosphoenolpyruvate-dependent phosphotransferase system. The nucleotide sequence encompassing the S. mutans lac promoter appears to contain repeat elements analogous to those of S. aureus, suggesting that repression and catabolite repression of the lactose operons may be similar in these organisms.
Rosey
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Department of Microbiology, University of Kansas, Lawrence 66045.
Stewart
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D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D001483:Base Sequence; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; D005798:Genes, Bacterial / Q000235:genetics*; D006600:Hexosephosphates / Q000378:metabolism*; D007763:Lac Operon / Q000235:genetics*; D007785:Lactose / Q000378:metabolism*; D026901:Membrane Transport Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D009004:Monosaccharide Transport Proteins; D009693:Nucleic Acid Hybridization; D011401:Promoter Regions, Genetic; D014176:Protein Biosynthesis; D011487:Protein Conformation; D012097:Repressor Proteins / Q000235:genetics*; D017385:Sequence Homology; D013295:Streptococcus mutans / Q000235:genetics*; D027981:Symporters
D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; C489070:DeoR protein, E coli; D029968:Escherichia coli Proteins; D006600:Hexosephosphates; C050221:LacY protein, E coli; D026901:Membrane Transport Proteins; D009004:Monosaccharide Transport Proteins; D012097:Repressor Proteins; D027981:Symporters; C026634:tagatose 6-phosphate; C021499:lactose permease; D007785:Lactose