title: Effects of starvation on valine and alanine transport across the intestinal mucosal border in sea bass, Dicentrarchus labrax.
abstract: Kinetics of intestinal transport of L-alanine and L-valine (substrates of the A-system and the L-system, respectively, in mammals) across the brush-border membrane in sea bass, Dicentrarchus labrax, were studied on intact mucosa using a short-term uptake technique. When fish were starved for 4-8 weeks, total influx (mucosa-to-cell) of valine fell owing to disappearance or modification of the diffusion component. The maximum influx rate of saturable component increased but its affinity (reflected by the Michaelis constant) decreased. Alanine transport by Na(+)-dependent and diffusion pathways was unchanged after starvation. Fasting also induced an almost 20% decrease in the length of intestinal microvilli.
authors.affiliation: Laboratoire de Physiologie Cellulaire et Comparée, URA CNRS 651, Université de Nice Sophia Antipolis, Faculté des Sciences, France.
authors.forename: M
authors.initials: M
authors.lastname: Avella
authors.forename: O
authors.initials: O
authors.lastname: Blaise
authors.forename: J
authors.initials: J
authors.lastname: Berhaut
mesh_terms: D000409:Alanine / Q000378:metabolism; D000818:Animals; D001492:Bass / Q000378:metabolism*; D001693:Biological Transport, Active; D066298:In Vitro Techniques; D007413:Intestinal Mucosa / Q000378:metabolism* / Q000648:ultrastructure; D007700:Kinetics; D008854:Microscopy, Electron; D008871:Microvilli / Q000378:metabolism / Q000648:ultrastructure; D013217:Starvation / Q000378:metabolism*; D014633:Valine / Q000378:metabolism
chemical_list: D014633:Valine; D000409:Alanine