title: Structure of the CD59-encoding gene: further evidence of a relationship to murine lymphocyte antigen Ly-6 protein.
abstract: The gene for CD59 [membrane inhibitor of reactive lysis (MIRL), protectin], a phosphatidylinositol-linked surface glycoprotein that regulates the formation of the polymeric C9 complex of complement and that is deficient on the abnormal hematopoietic cells of patients with paroxysmal nocturnal hemoglobinuria, consists of four exons spanning 20 kilobases. The untranslated first exon is preceded by a G+C-rich promoter region that lacks a consensus TATA or CAAT motif. The second exon encodes the hydrophobic leader sequence of the protein, and the third exon encodes the amino-terminal portion of the mature protein. The fourth exon encodes the remainder of the mature protein, including the hydrophobic sequence necessary for glycosyl-phosphatidylinositol anchor attachment. The structure of the CD59 gene is very similar to that encoding Ly-6, a murine glycoprotein with which CD59 has some structural similarity. The striking similarity in gene structure is further evidence that the two proteins belong to a superfamily of proteins that may also include the urokinase plasminogen-activator receptor and a squid glycoprotein of unknown function.
authors.affiliation: Department of Medicine, Duke University Medical Center, Durham, NC 27710.
authors.forename: J G
authors.initials: JG
authors.lastname: Petranka
authors.forename: D E
authors.initials: DE
authors.lastname: Fleenor
authors.forename: K
authors.initials: K
authors.lastname: Sykes
authors.forename: R E
authors.initials: RE
authors.lastname: Kaufman
authors.forename: W F
authors.initials: WF
authors.lastname: Rosse
mesh_terms: D000595:Amino Acid Sequence; D015703:Antigens, CD / Q000235:genetics*; D000950:Antigens, Ly / Q000235:genetics; D001483:Base Sequence; D018957:CD59 Antigens; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D005796:Genes; D006801:Humans; D008562:Membrane Glycoproteins / Q000235:genetics*; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D015183:Restriction Mapping; D016415:Sequence Alignment
chemical_list: D015703:Antigens, CD; D000950:Antigens, Ly; D018957:CD59 Antigens; D008562:Membrane Glycoproteins; D004247:DNA