Antigenic and genetic analyses of human rotaviruses in Chiang Mai, Thailand: evidence for a close relationship between human and animal rotaviruses.
Serotyping of group A rotaviruses obtained from stools of 158 diarrheic patients in Chiang Mai, Thailand, by ELISA with monoclonal antibodies revealed a yearly change in the prevalence of individual serotypes. Three unusual human rotavirus strains were isolated. Strain Mc35 had subgroup I-serotype 10 antigen and a long RNA electrophoretic type, a property hitherto found only in bovine rotaviruses. RNA-RNA hybridization tests showed that the strain is more closely related genetically to bovine than to human rotaviruses. Strain Mc323, although serologically closely related to serotype 9, had subgroup I specificity and a long RNA electrophoretic type, a characteristic common to nonhuman rotaviruses. Strain Mc345, with an aberrant RNA pattern possibly due to genome rearrangement, had the same antigenic specificity as Mc323. These 2 strains were genetically very closely related to each other and were more related to porcine than to human rotaviruses. These results provide insights into the evolutionary mechanisms of human rotaviruses.
Urasawa
S
S
Department of Hygiene and Epidemiology, Sapporo Medical College, Japan.
Hasegawa
A
A
Urasawa
T
T
Taniguchi
K
K
Wakasugi
F
F
Suzuki
H
H
Inouye
S
S
Pongprot
B
B
Supawadee
J
J
Suprasert
S
S
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis*; D002675:Child, Preschool; D003968:Diarrhea, Infantile / Q000382:microbiology*; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000032:analysis; D005243:Feces / Q000382:microbiology; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D009500:Neutralization Tests; D009693:Nucleic Acid Hybridization; D015347:RNA Probes; D012330:RNA, Double-Stranded / Q000032:analysis; D012367:RNA, Viral / Q000032:analysis*; D012401:Rotavirus / Q000145:classification* / Q000235:genetics / Q000276:immunology; D012400:Rotavirus Infections / Q000382:microbiology*; D012703:Serotyping; D013045:Species Specificity; D013785:Thailand
D000956:Antigens, Viral; D000939:Epitopes; D015347:RNA Probes; D012330:RNA, Double-Stranded; D012367:RNA, Viral