Determination of B-cell epitopes of nef HIV-I protein: immunogenicity related to their structure.
Determination of the B-cell epitopes of the nef molecule encoded by the human immunodeficiency virus type 1 (HIV-1) was undertaken using a set of six synthetic peptides. Sequences that were both antigenic and immunogenic and stimulated the production of antibodies recognizing the full length molecule, were considered as B-cell epitopes. Two peptidic sequences were antigenic both in rodents (mice and rats) and in non-human primates (chimpanzee). They were located in the regions 45-69 and 176-206 of the nef molecule. Two additional antigenic sequences were determined, one in chimpanzees, region 79-94, and the second in rodents, region 148-161. Immunogenicity was investigated in the rodents. Only the 45-69 and 176-206 sequences were immunogenic, and specific antibodies present in the sera of the immunized animals reacted with the nef protein. Therefore, each of these two sequences could be considered as containing at least one B-cell epitope. The fine epitopic specificity was determined using subfragments of these two sequences and it was shown that the antigenic determinants were contained in the C-terminal region of each sequence overlapping with the T-cell epitopes. These results raised the importance of vicinity of B- and T-cell determinants and their immunogenicity.
Estaquier
J
J
Centre d'Immunologie des maladies transmissibles et allergiques, Unité Mixte INSERM U167-CNRS 624, Institut Pasteur, Lille, France.
Boutillon
C
C
Ameisen
J C
JC
Gras-Masse
H
H
Delanoye
A
A
Lecocq
J P
JP
Dixson
A
A
Tartar
A
A
Capron
A
A
Auriault
C
C
D000595:Amino Acid Sequence; D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D015153:Blotting, Western; D002352:Carrier Proteins; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000235:genetics / Q000276:immunology*; D005260:Female; D015693:Gene Products, nef / Q000276:immunology*; D015497:HIV-1; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D002679:Pan troglodytes; D051381:Rats; D011916:Rats, Inbred F344; D013997:Time Factors; D014611:Vaccination; D054311:nef Gene Products, Human Immunodeficiency Virus
D002352:Carrier Proteins; D000939:Epitopes; D015693:Gene Products, nef; D007074:Immunoglobulin G; D054311:nef Gene Products, Human Immunodeficiency Virus