Anthracycline metabolites from baumycin-producing Streptomyces sp. D788. I. Isolation of antibiotic-blocked mutants and their characterization.
Biosynthetically blocked mutants were obtained from a baumycin-producing Streptomyces sp. D788 newly isolated from soil. The first mutant isolated was a baumycin-negative but daunorubicin-accumulating mutant with a loss of 4'-substitution activity, from which all other blocked mutants were successively derived. These included a known 11-deoxydaunorubicin-producing mutant and several new types of mutants which produced mainly 10-carboxy-13-deoxocarminomycin, 10-methoxycarbonyl-13-deoxocarminomycin, their 11-deoxy derivatives or a precursor aglycone, respectively. In this paper, all the anthracycline components produced by the parent strain and its two known blocked mutants, a daunorubicin producer and a 11-deoxydaunorubicin producer, are also determined by HPLC and five new components are isolated. Cytotoxicities in vitro of all the components against L1210 cell culture are also described.
Yoshimoto
A
A
Central Research Laboratories, Mercian Corporation, Fujisawa, Japan.
Johdo
O
O
Fujii
S
S
Kubo
K
K
Nishida
H
H
Okamoto
R
R
Takeuchi
T
T
D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D003630:Daunorubicin / Q000031:analogs & derivatives* / Q000096:biosynthesis; D007939:Leukemia L1210 / Q000473:pathology; D051379:Mice; D009154:Mutation; D013302:Streptomyces / Q000235:genetics / Q000378:metabolism*; D014407:Tumor Cells, Cultured / Q000187:drug effects
D000903:Antibiotics, Antineoplastic; C015018:baumycins; D003630:Daunorubicin