title: Efficient purification of PCR products using ultrafiltration.
abstract: The use of disposable ultrafiltration devices for the purification of PCR-amplified DNA products from the other components of the reaction mixture is outlined. The advantages of using a 100,000 molecular weight cutoff membrane are discussed. The integrity of the membrane-purified DNA is checked by its successful use in sequencing, ligation and cloning procedures.
authors.affiliation: Amicon, Inc., Beverly, MA 01915-1065.
authors.forename: A M
authors.initials: AM
authors.lastname: Krowczynska
authors.forename: M B
authors.initials: MB
authors.lastname: Henderson
mesh_terms: D004247:DNA / Q000302:isolation & purification*; D004279:DNA, Viral / Q000302:isolation & purification; D016133:Polymerase Chain Reaction / Q000379:methods*; D014462:Ultrafiltration / Q000295:instrumentation
chemical_list: D004279:DNA, Viral; D004247:DNA