Homeostasis as regulated by activated macrophage. VIII. LPSw (a lipopolysaccharide from wheat flour) can regulate bone resorption of chick embryo.
The effect of LPSw (a lipopolysaccharide from wheat flour) on the bone resorption of 18-d chick embryonic calvaria was examined in an organ culture following the method of Raisz. Bone was prelabeled in culture medium containing 45Ca and chased in a cold medium. On addition of test samples, labeled calcium was released indicating the grade of bone resorption. LPSw (10-100 ng/ml) stimulated bone resorption, showing an effect comparable to parathyroid hormone (PTH) (1 U/ml). PTH at 1 U/ml decreased the total amount of calcium and phosphorus, while LPSw did not. LPSw is thus assumed to stimulate bone resorption more actively than PTH.
Kawashima
K
K
Department of Physiological Chemistry, Faculty of Pharmaceutical Sciences, Teikyo University, Kanagawa, Japan.
Endo
H
H
Nishizawa
T
T
Inagawa
H
H
Okutomi
T
T
Morikawa
A
A
Soma
G
G
Mizuno
D
D
D000818:Animals; D001862:Bone Resorption / Q000188:drug therapy* / Q000503:physiopathology; D002642:Chick Embryo; D006706:Homeostasis / Q000187:drug effects / Q000502:physiology*; D008070:Lipopolysaccharides / Q000627:therapeutic use*; D008262:Macrophage Activation / Q000187:drug effects / Q000502:physiology*; D008264:Macrophages / Q000187:drug effects / Q000502:physiology; D009924:Organ Culture Techniques; D012886:Skull / Q000196:embryology / Q000503:physiopathology; D014908:Triticum
D008070:Lipopolysaccharides