title: Milk-stimulated PGE2 production by isolated gastric cells: a possible role in the inhibition of histamine-induced acid secretion.
abstract: The effects of a milk diet on gastric acid secretion of rats fed raw bovine milk for 4 days were examined using dispersed gastric cells. Parietal cell acid secretion was estimated by the accumulation of 14C-aminopyrine (AP), an index of secretory function. Basal AP accumulation was significantly increased (60%) by the milk diet. There was a marked upward shift in the dose-response curve of histamine (HA; 10(-8) to 10(-3) M) in milk-fed rats, indicating enhanced sensitivity of parietal cell-H2 receptor to exogenous HA. In contrast, the dose-dependent inhibition of HA-induced AP accumulation by prostaglandin (PG) E2 was significantly reduced, indicating that the parietal cells of milk-fed rats were less sensitive to exogenous PGE2. The PGE2 content of bovine milk was low (less than 20 pg/ml), but the production of endogenous PGE2 by the gastric cells was dramatically increased by the milk diet and exhibited maximal control production rate in the presence of 10 microM arachidonic acid. The increased responsiveness to histamine and the decreased responsiveness to PGE2 indicated that the milk diet induced low histamine and high PGE2 availability in the vicinity of the parietal cell basolateral membrane. This regulation, which involves stimulation of PGE2 production in the gastric mucosa, may underly the inhibition of acid secretion observed in vivo in chronically milk-fed adult rats.
authors.affiliation: INSERM U. 319, Laboratoire de Différenciation Fonctionnelle, Université Paris 7, France.
authors.forename: R
authors.initials: R
authors.lastname: Ducroc
authors.forename: A
authors.initials: A
authors.lastname: Wirbel
authors.forename: B
authors.initials: B
authors.lastname: Garzon
authors.forename: J
authors.initials: J
authors.lastname: Vilar
authors.forename: B
authors.initials: B
authors.lastname: Brunel-Riveau
authors.forename: J P
authors.initials: JP
authors.lastname: Geloso
mesh_terms: D000632:Aminopyrine / Q000378:metabolism; D000818:Animals; D002250:Carbon Radioisotopes; D015232:Dinoprostone / Q000096:biosynthesis*; D005744:Gastric Acid / Q000378:metabolism*; D005753:Gastric Mucosa / Q000378:metabolism*; D006632:Histamine / Q000494:pharmacology*; D066298:In Vitro Techniques; D008297:Male; D008892:Milk; D051381:Rats; D013270:Stomach / Q000166:cytology
chemical_list: D002250:Carbon Radioisotopes; D000632:Aminopyrine; D006632:Histamine; D015232:Dinoprostone