Hypertonic stress induces alpha B-crystallin expression.
Alpha B-crystallin, a major lens protein, was induced in primary cultures of dog lens epithelial cells and glomerular endothelial cells when they were grown under conditions of hypertonic stress. With Western blot analysis using a specific alpha B-crystallin antibody, we observed a significant increase in the concentration of alpha B-crystallin protein in cells grown for 4-6 days in media supplemented with 150 mM NaCl or 250 mM cellobiose. These supplements increased the osmolarity of the medium from 300 to 550-600 mosmol kg-1. Alpha B-crystallin mRNA was also increased reaching a maximum four-fold increase in lens and 16-fold increase in kidney cells within 1-2 days. These studies demonstrate a type of regulation of alpha B-crystallin expression in cells from lenticular and non-lenticular tissues.
Dasgupta
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S
National Eye Institute, National Institutes of Health, Bethesda, MD 20892.
Hohman
T C
TC
Carper
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D
D000818:Animals; D015153:Blotting, Western; D002478:Cells, Cultured; D003459:Crystallins / Q000235:genetics / Q000378:metabolism*; D004285:Dogs; D004591:Electrophoresis, Polyacrylamide Gel; D004727:Endothelium / Q000378:metabolism; D004848:Epithelium / Q000378:metabolism; D005786:Gene Expression Regulation; D006982:Hypertonic Solutions; D007678:Kidney Glomerulus / Q000378:metabolism; D007908:Lens, Crystalline / Q000378:metabolism*; D009994:Osmolar Concentration; D012313:RNA / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D013312:Stress, Physiological
D003459:Crystallins; D006982:Hypertonic Solutions; D012333:RNA, Messenger; D012313:RNA