Development of a flow-cytometric HLA-A locus mutation assay for human peripheral blood lymphocytes.
A flow-cytometric technique was developed to measure the frequency of variant lymphocytes lacking expression of HLA-A2 or A24 allele products among donors heterozygous for HLA-A2 or A24. It was found that the variant frequency of lymphocytes in peripheral blood was of the order of 10(-4) and increased with donor age. Molecular analyses of mutant clones revealed that about one-third were derived from somatic recombinations and that the remaining two-thirds did not show any alterations after Southern blotting analysis. In contrast, mutants obtained after in vitro X-ray mutagenesis study were found to be mostly derived from large chromosomal deletions. A small-scale study on atomic bomb survivors did not show a significant dose effect.
Kushiro
J
J
Department of Radiobiology, Radiation Effects Research Foundation, Hiroshima, Japan.
Hirai
Y
Y
Kusunoki
Y
Y
Kyoizumi
S
S
Kodama
Y
Y
Wakisaka
A
A
Jeffreys
A
A
Cologne
J B
JB
Dohi
K
K
Nakamura
N
N
D000328:Adult; D000368:Aged; D015139:Blotting, Southern; D015197:Carcinogenicity Tests; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D005434:Flow Cytometry / Q000379:methods*; D005805:Genes, MHC Class I / Q000235:genetics*; D015234:HLA-A Antigens / Q000235:genetics*; D015789:HLA-A2 Antigen / Q000235:genetics*; D059871:HLA-A24 Antigen; D006801:Humans; D008214:Lymphocytes / Q000528:radiation effects; D008875:Middle Aged; D016296:Mutagenesis / Q000235:genetics; D009152:Mutagenicity Tests; D009154:Mutation / Q000235:genetics; D011995:Recombination, Genetic / Q000235:genetics
D015234:HLA-A Antigens; D015789:HLA-A2 Antigen; D059871:HLA-A24 Antigen