Interleukin 3 protects murine bone marrow cells from apoptosis induced by DNA damaging agents.
Murine bone marrow-derived cells, dependent on interleukin 3 (IL-3) for their growth in culture, undergo programmed cell, or apoptosis, upon cytokine withdrawal. Here it is reported that a variety of DNA damaging agents cause a more rapid onset of apoptosis in a factor-dependent cell line, BAF3, deprived of IL-3. In contrast, when cultured in the presence of IL-3, or other growth promoting factors, BAF3 cells are highly resistant to X-irradiation and the cytotoxic drugs etoposide and cisplatin. Overexpression of the bcl2 gene product also protects BAF3 cells from DNA damage. The presence of IL-3 is not required during the initial events of DNA damage or its repair. In the absence of IL-3, cells still complete the repair of DNA breaks within 15 min, and continue to cycle for 5 h. At this time, IL-3 is necessary to prevent the accelerated onset of DNA cleavage from a G2 arrest point.
Collins
M K
MK
Chester Beatty Laboratories, Institute of Cancer Research, London, United Kingdom.
Marvel
J
J
Malde
P
P
Lopez-Rivas
A
A
D000818:Animals; D017209:Apoptosis / Q000187:drug effects* / Q000528:radiation effects; D001853:Bone Marrow / Q000187:drug effects / Q000528:radiation effects; D001854:Bone Marrow Cells; D002453:Cell Cycle / Q000187:drug effects; D002478:Cells, Cultured; D002945:Cisplatin / Q000494:pharmacology*; D004247:DNA / Q000187:drug effects* / Q000528:radiation effects; D004249:DNA Damage; D005047:Etoposide / Q000494:pharmacology*; D007377:Interleukin-3 / Q000494:pharmacology*; D007700:Kinetics; D051379:Mice
D007377:Interleukin-3; D005047:Etoposide; D004247:DNA; D002945:Cisplatin