Duodenogastric reflux enhances growth and carcinogenesis in the rat pancreas.
Surgery for peptic ulcer disease may increase the risk of pancreatic cancer. The effect of duodenogastric reflux on pancreatic carcinogenesis was tested, and changes in the circulating levels of cholecystokinin (CCK) and gastrin were measured. Male Wistar rats (n = 40) weighing 250-300 g were randomized to undergo gastrotomy (control) or split gastrojejunostomy (to produce complete duodenogastric reflux) and then to receive azaserine (30 mg/kg/week intraperitoneally) or saline injections for 3 weeks. At 6 months, blood CCK was assayed and the pancreas was excised for quantitative estimation of atypical acinar cell foci (AACF), the precursor lesions of carcinoma. Rats that had undergone split gastrojejunostomy weighed 15-19 per cent less than controls (P < 0.05), but their relative pancreatic weight (mg pancreas per 100 g body-weight) was 52-60 per cent greater (P < 0.001). Acidophilic AACF occurred only in azaserine-treated rats with duodenogastric reflux. Although plasma CCK concentrations were unchanged, split gastrojejunostomy increased basal and postprandial gastrin levels by 98-175 per cent (P < 0.05). Duodenogastric reflux produces sustained hypergastrinaemia and promotes experimental pancreatic carcinogenesis.
Watanapa
P
P
Department of Surgery, Royal Postgraduate Medical School, Hammersmith Hospital, London, UK.
Flaks
B
B
Oztas
H
H
Deprez
P H
PH
Calam
J
J
Williamson
R C
RC
D000714:Anastomosis, Surgical / Q000009:adverse effects; D000818:Animals; D002766:Cholecystokinin / Q000097:blood; D004383:Duodenogastric Reflux / Q000097:blood / Q000150:complications*; D005755:Gastrins / Q000097:blood; D007583:Jejunum / Q000601:surgery*; D008297:Male; D010190:Pancreatic Neoplasms / Q000097:blood / Q000209:etiology*; D011183:Postoperative Complications / Q000097:blood; D051381:Rats; D011919:Rats, Inbred Strains; D013270:Stomach / Q000601:surgery*
D005755:Gastrins; D002766:Cholecystokinin