[Evaluation of the alkaline-phosphatase labeled DNA probes for Mycobacterium tuberculosis and Mycobacterium avium complex].
Non-radioisotopic, alkaline phosphatase-labeled DNA (AP-DNA) probe tests for the identification of Mycobacterium tuberculosis complex (Mtb) and Mycobacterium avium complex (MAC) were evaluated. The overall agreement, sensitivity and specificity of the AP-DNA probes for Mtb and MAC were 100% respectively compared with the conventional biochemical method. Because the procedure is rapid (it can be completed approximately 120 min), safe (it does not use radioisotopes) and convenient (it does not need the special equipment to be performed), it can be easily performed in any clinical laboratory.
Okada
J
J
Clinical Laboratory, Kanto Teishin Hospital.
Kaetsu
M
M
D000469:Alkaline Phosphatase; D015342:DNA Probes; D006801:Humans; D015269:Mycobacterium avium Complex / Q000302:isolation & purification*; D009169:Mycobacterium tuberculosis / Q000302:isolation & purification*; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity
D015342:DNA Probes; D000469:Alkaline Phosphatase