[Effect of d-catechin on carbon tetrachloride- and d-galactosamine-induced cytotoxicity in primary cultured rat hepatocytes].
The effects of d-catechin (d-CTC) on carbon tetrachloride- and d-galactosamine (d-Ga1N)-induced cytotoxicity in primary cultured rat hepatocytes were examined. After 1.5 h preincubation, d-CTC was added at doses of 0.1-5.0 mg/ml to culture medium together with 10 mmol/L CCl4 or 5 mmol/L d-GalN, respectively. GOT, GPT and LDH levels were measured 1.5 h after treatment. The results showed that d-CTC at doses of 0.6 to 5.0 mg/ml could protect the hepatocytes against the toxic effects of CCl4 and d-GalN. At the higher doses (2.5-5.0 mg/ml), d-CTC showed weak inhibition of LDH and GPT activities, but these did not influence its anti-hepatotoxic activity.
Fang
R
R
Zhejiang Branch, Hangzhou.
D000818:Animals; D001219:Aspartate Aminotransferases / Q000378:metabolism; D002251:Carbon Tetrachloride / Q000633:toxicity; D002392:Catechin / Q000494:pharmacology*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005688:Galactosamine / Q000633:toxicity; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008099:Liver / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013237:Stereoisomerism
D005688:Galactosamine; D002392:Catechin; D002251:Carbon Tetrachloride; D007770:L-Lactate Dehydrogenase; D001219:Aspartate Aminotransferases