Nanogram level quantitation of oxycodone in human plasma by capillary gas chromatography using nitrogen-phosphorus selective detection.
A sensitive and specific capillary gas chromatographic assay is reported for the quantitation of oxycodone in human plasma. The technique involves a single extraction of oxycodone and internal standard (hydrocodone) from plasma by toluene containing 1% isopropanol. Separation is achieved on a methyl silicone (HP-1) fused-silica capillary column (25 m x 0.2 mm I.D., 0.33 microns film thickness) and detection is by nitrogen-phosphorus selective mode. The minimum quantifiable limit is 1.8 ng/ml using 2 ml of plasma. The method is applicable to characterize the plasma profile of oxycodone in humans after a single oral 5-mg oxycodone hydrochloride tablet.
Kapil
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Drug Metabolism and Pharmacokinetics Section, Stine-Haskell Research Center, Du Pont Merck Pharmaceutical Company, Newark, DE 19714.
Padovani
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King
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Lam
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D002849:Chromatography, Gas; D006801:Humans; D006853:Hydrocodone / Q000097:blood; D008297:Male; D009584:Nitrogen; D010098:Oxycodone / Q000097:blood*; D010758:Phosphorus; D015203:Reproducibility of Results
D010758:Phosphorus; D006853:Hydrocodone; D010098:Oxycodone; D009584:Nitrogen