Complement activation and adsorption of protein fragments by functionalized polymer surfaces in human serum.
The interactions between blood and polymer surfaces used in extracorporeal circulations result in variable activations of the immune system of complement. Measuring concentrations of C3a or C5a in supernatant blood or serum after contact with the surface has been the most usual way of assessing this activation. Most polymer surfaces bearing various chemical groups were found to adsorb C3a and sometimes C5a. After taking into account adsorption, a good correlation was found between total C3a generated and CH50 units consumed by most of the polymer samples tested. Measuring only C3a remaining in the fluid phase should not be considered sufficient to conclude that a material surface is not an activator of complement.
Montdargent
B
B
Laboratorie de Recherches sur les Macromolécules, CNRS URA D0502, Université Paris-Nord, Villetaneuse, France.
Toufik
J
J
Carreno
M P
MP
Labarre
D
D
Jozefowicz
M
M
D000327:Adsorption; D003167:Complement Activation / Q000187:drug effects*; D015926:Complement C3a / Q000031:analogs & derivatives / Q000096:biosynthesis / Q000378:metabolism; D003911:Dextrans / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D010446:Peptide Fragments / Q000097:blood / Q000493:pharmacokinetics*; D011108:Polymers / Q000737:chemistry / Q000493:pharmacokinetics*; D011137:Polystyrenes / Q000737:chemistry / Q000494:pharmacology; D013499:Surface Properties
D003911:Dextrans; D010446:Peptide Fragments; D011108:Polymers; D011137:Polystyrenes; C044541:complement C3a, des-Arg-(77)-; D015926:Complement C3a; C025614:sephadex