Identification of restriction-fragment length polymorphisms for the human chorionic gonadotropin-beta/luteinizing hormone-beta gene cluster.
OBJECTIVE
To determine whether restriction fragment length polymorphisms are present using a deoxyribonucleic acid (DNA) probe for human luteinizing hormone beta subunit (hLH-beta). If the gene for hLH-beta is polymorphic, genetic diagnosis of disorders of luteinizing hormone (hLH) and human chorionic gonadotropin (hCG) production could become possible.


METHODS
Study of genomic DNA from controls with a variety of restriction enzymes to identify polymorphisms.


METHODS
Laboratories of the Department of Obstetrics and Gynecology, Department of Oral Biology, Medical College of Georgia, Augusta, Georgia.


METHODS
Unrelated control men and women seen in clinics at the Medical College of Georgia.


METHODS
Genomic DNA was extracted from patients and digested with eight different restriction enzymes for the study of the hLH-beta gene by Southern analysis.


METHODS
Fragment (band) sizes on radiographs from Southern blots were compared with those from molecular weight standards.


CONCLUSIONS
Restriction fragment length polymorphisms were identified for four of the restriction enzymes, DraI, HincII, MboI, and KpnI. These polymorphisms may be useful in the diagnosis of disorders of hLH and hCG production.
Roach
D J
DJ
Department of Obstetrics and Gynecology, Medical College of Georgia, Augusta.
Layman
L C
LC
McDonough
P G
PG
Lanclos
K D
KD
Wall
S W
SW
Wilson
J T
JT
D006063:Chorionic Gonadotropin / Q000235:genetics*; D018997:Chorionic Gonadotropin, beta Subunit, Human; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D005260:Female; D006801:Humans; D007986:Luteinizing Hormone / Q000235:genetics*; D008297:Male; D010446:Peptide Fragments / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length
D006063:Chorionic Gonadotropin; D018997:Chorionic Gonadotropin, beta Subunit, Human; D010446:Peptide Fragments; D007986:Luteinizing Hormone; D015252:Deoxyribonucleases, Type II Site-Specific; C024405:GATC-specific type II deoxyribonucleases; C029312:GGTACC-specific type II deoxyribonucleases; C024400:GTYRAC-specific type II deoxyribonucleases; C035594:TTTAAA -specific type II deoxyribonucleases