title: Normal aggregations of glycoprotein IV (CD36)-deficient platelets from seven healthy Japanese donors.
abstract: Since glycoprotein IV (GPIV) has been shown to play an important role in the interaction of platelets with collagen and thrombospondin, the aggregation and secretion of GPIV-deficient platelets were examined. Using a binding assay with monoclonal 125I-OKM5 antibody against CD36 antigen and crossed immunoelectrophoresis of the solubilized platelets against anti-GPIV antibody, the platelets from seven (4.1%) out of 170 healthy Japanese donors were found to be deficient in GPIV. The GPIV-deficient platelets showed normal aggregations in response to collagen as well as ADP, epinephrine, arachidonic acid and thrombin in comparison with GPIV-positive platelets. Polyclonal anti-GPIV antibody aggregated GPIV-positive platelets but not the GPIV-negative ones. The F(ab')2 fragments of the anti-GPIV antibody competitively inhibited the anti-GPIV-induced aggregation, but did not affect the collagen-induced aggregation of GPIV-positive platelets. These results suggest that the deficiency of GPIV does not affect platelet aggregability.
authors.affiliation: Department of Cardiovascular Research, Tokyo Metropolitan Institute of Medical Science, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Yamamoto
authors.forename: N
authors.initials: N
authors.lastname: Akamatsu
authors.forename: H
authors.initials: H
authors.lastname: Yamazaki
authors.forename: K
authors.initials: K
authors.lastname: Tanoue
mesh_terms: D000244:Adenosine Diphosphate / Q000494:pharmacology; D000293:Adolescent; D000328:Adult; D000595:Amino Acid Sequence; D015703:Antigens, CD / Q000032:analysis*; D016718:Arachidonic Acid / Q000494:pharmacology; D001792:Blood Platelets / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism; D018955:CD36 Antigens; D003094:Collagen / Q000494:pharmacology; D004837:Epinephrine / Q000494:pharmacology; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007122:Immunoelectrophoresis; D007140:Immunoglobulin Fab Fragments / Q000502:physiology; D007457:Iodine Radioisotopes; D007564:Japan / Q000453:epidemiology; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D010974:Platelet Aggregation / Q000187:drug effects / Q000502:physiology*; D011488:Protein Deficiency / Q000097:blood / Q000453:epidemiology / Q000473:pathology; D013917:Thrombin / Q000494:pharmacology
chemical_list: D015703:Antigens, CD; D018955:CD36 Antigens; D007140:Immunoglobulin Fab Fragments; D007457:Iodine Radioisotopes; D016718:Arachidonic Acid; D000244:Adenosine Diphosphate; D003094:Collagen; D013917:Thrombin; D004837:Epinephrine