Determination of urinary beta-phenylethylamine as its N-benzenesulphonamide derivative by gas chromatography with flame photometric detection.
A selective and sensitive method for the determination of urinary beta-phenylethylamine (PEA) by gas chromatography (GC) has been developed. After extraction of the urine sample with n-pentane, PEA was converted into its N-benzenesulphonamide derivative and then determined by GC with flame photometric detection using a DB-1301 capillary column. By using this method, nanogram amounts of PEA in urine could be accurately and precisely determined without any influence from coexisting substances. Analytical results for the determination of PEA in urine samples from normal subjects are presented.
Kataoka
H
H
Faculty of Pharmaceutical Sciences, Okayama University, Japan.
Ohrui
S
S
Miyamoto
Y
Y
Makita
M
M
D002849:Chromatography, Gas / Q000379:methods; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D010420:Pentanes; D010627:Phenethylamines / Q000652:urine*; D013053:Spectrophotometry; D013449:Sulfonamides; D000096926:Benzenesulfonamides
D010420:Pentanes; D010627:Phenethylamines; D013449:Sulfonamides; D000096926:Benzenesulfonamides; C029261:phenethylamine; C033353:pentane