Primary culture of liver cancer tissues with or without transcatheter arterial embolization and establishment of a cell strain.
More epithelial-like cells are found in primary cultures of transcathetel arterial embolization (TAE)-untreated human liver cancer tissues than in those of TAE-treated tissues. A new cell strain, HUH-33, established from the former, grows slowly and is untransplantable into nude mice and secretes alpha-fetoprotein, albumin and some other tumor markers. Chromosome numbers are widely distributed. HUH-33 expresses hepatocyte type keratin and contains desmosome- or intermediate filament bundle-like structures.
Tokiwa
T
T
Department of Cell Biology, Okayama University School of Medicine, Japan.
Kusaka
Y
Y
Endo
A
A
Namba
M
M
D000368:Aged; D014408:Biomarkers, Tumor / Q000378:metabolism; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000648:ultrastructure; D002460:Cell Line / Q000378:metabolism; D003896:Desmosomes / Q000648:ultrastructure; D004621:Embolization, Therapeutic; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007382:Intermediate Filaments / Q000648:ultrastructure; D007621:Karyotyping; D007633:Keratins / Q000096:biosynthesis; D008113:Liver Neoplasms / Q000235:genetics* / Q000648:ultrastructure; D008297:Male; D008875:Middle Aged; D014407:Tumor Cells, Cultured / Q000378:metabolism
D014408:Biomarkers, Tumor; D007633:Keratins