Molecular basis of antigenic structures of poliovirus: implications for their evolution during morphogenesis.
Neutralizing monoclonal antibodies against poliovirus type 1 were obtained after conventional immunization or combined in vivo-in vitro immunization. Antibody binding sites were determined by sequence analysis of neutralization-resistant mutants. Site 3 variants had several amino acid substitutions in previously unidentified positions for neutralization resistance. Evidence for a linkage of subsites 3a and 3b is presented. Some site 3b antibodies as defined previously precipitated 14S subunits, although with reduced titers.
Wiegers
K
K
Heinrich-Pette-Institut für Experimentelle Virologie Universität Hamburg, Germany.
Dernick
R
R
D000911:Antibodies, Monoclonal / Q000276:immunology; D000956:Antigens, Viral / Q000737:chemistry / Q000276:immunology*; D000939:Epitopes; D009024:Morphogenesis; D009154:Mutation; D009500:Neutralization Tests; D017955:Poliovirus / Q000276:immunology*; D011233:Precipitin Tests
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D000939:Epitopes