title: Partial pressures of oxygen and carbon dioxide in bone and their correlation with bone-blood flow: effect of decreased arterial supply and venous congestion on intraosseous oxygen and carbon dioxide in an animal model.
abstract: Pathological changes in bone have been related to a preceding impediment of the arterial or venous bone circulation and hypoxia. In this study, we analyzed the feasibility of mass spectrometry in measuring intraosseous oxygen and carbon dioxide. The partial pressures were also measured in intraosseous blood samples, and blood flow in bone was measured with the radioactive microspheres technique. The average partial pressure of oxygen in the lateral femoral condyle was 34 +/- 1.6 mm Hg when measured in intraosseous blood samples and 36.3 +/- 2.3 mm Hg when measured with the on-line mass spectrometer, with significant correlation between the methods. The absolute value of the partial pressure of carbon dioxide measured in situ with mass spectrometry was correlated with the value in the withdrawn blood. There was no significant difference in partial pressures of oxygen and carbon dioxide between the two sides or between repetitive measurements. Arterial occlusion resulted in severe hypoxia, whereas more moderate changes followed venous occlusion.
authors.affiliation: Department of Orthopaedic Surgery U, Rigshospitalet, Copenhagen, Denmark.
authors.forename: T
authors.initials: T
authors.lastname: Kiaer
authors.forename: B
authors.initials: B
authors.lastname: Dahl
authors.forename: G
authors.initials: G
authors.lastname: Lausten
mesh_terms: D000818:Animals; D001842:Bone and Bones / Q000098:blood supply* / Q000378:metabolism; D002245:Carbon Dioxide / Q000032:analysis; D005269:Femur / Q000098:blood supply; D000860:Hypoxia; D013058:Mass Spectrometry; D008863:Microspheres; D010092:Oximetry / Q000379:methods*; D010100:Oxygen / Q000032:analysis*; D010313:Partial Pressure; D012039:Regional Blood Flow; D013552:Swine; D014661:Vasoconstriction
chemical_list: D002245:Carbon Dioxide; D010100:Oxygen