title: A peptidergic component to vagally induced tracheal vasodilation in the dog.
abstract: The purpose of the study was to determine the extent that peptidergic afferent and efferent pathways contribute to vagally induced vasodilation in the trachea of the dog. The change in vascular resistance of the tracheal branch of the cranial thyroid artery and the trachealis responses were determined in 28 anesthetized, paralyzed, and mechanically ventilated dogs. After propranolol (2 mg/kg) and phentolamine (1.5 mg/kg), stimulation of the superior laryngeal nerves (NS; 15 Hz, 7 V, 2 ms, 30 s) caused a decrease in vascular resistance of 11.7 +/- 0.8% and a tracheal contraction of 5.2 +/- 4.7 cmH2O. Atropine (1.5 mg/kg) reduced the fall in vascular resistance to 4.7 +/- 0.8% (P less than 0.01), whereas tracheal contraction was abolished. Thiorphan (1.5 mg), a neutral endopeptidase inhibitor, augmented the decrease in vascular resistance (8.8 +/- 0.6%; P less than 0.01) to NS. After hexamethonium (0.5 mg/kg), NS still caused a small decrease in TVR (2.9 +/- 0.9%; P less than 0.05), which was abolished by capsaicin. In atropinized dogs, capsaicin reduced the fall in vascular resistance after NS; the residual vasodilation was virtually abolished by hexamethonium. Acetylcholine (10(-3) mg/kg) decreased vascular resistance (15.7 +/- 3.0%), and the effect was abolished by atropine. We conclude that there is noncholinergic nonadrenergic vagally induced tracheal vasodilation that is peptidergic. The peptidergic vasodilation appears to be mediated by both afferent and efferent pathways.
authors.affiliation: Meakins-Christie Laboratories, McGill University, Montreal, Quebec, Canada.
authors.forename: T
authors.initials: T
authors.lastname: Ito
authors.forename: T
authors.initials: T
authors.lastname: Takubo
authors.forename: S
authors.initials: S
authors.lastname: Hussain
authors.forename: J G
authors.initials: JG
authors.lastname: Martin
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology; D000344:Afferent Pathways / Q000502:physiology; D000818:Animals; D001285:Atropine / Q000494:pharmacology; D004285:Dogs; D004525:Efferent Pathways / Q000502:physiology; D004558:Electric Stimulation; D005260:Female; D007823:Laryngeal Nerves / Q000502:physiology; D008297:Male; D010455:Peptides / Q000502:physiology*; D014132:Trachea / Q000098:blood supply* / Q000294:innervation; D014630:Vagus Nerve / Q000502:physiology; D014655:Vascular Resistance / Q000502:physiology; D014660:Vasoactive Intestinal Peptide / Q000494:pharmacology / Q000502:physiology; D014664:Vasodilation / Q000187:drug effects / Q000502:physiology*
chemical_list: D010455:Peptides; D014660:Vasoactive Intestinal Peptide; D001285:Atropine; D000109:Acetylcholine