Primary structure and in vitro expression of the N. crassa phosphoglycerate kinase.
The primary structure of 3-phosphoglycerate kinase (PGK) from Neurospora crassa was determined by sequencing a full-length cDNA. The deduced 418 amino acids protein shows a considerable identity to PGKs of other organisms with all the residues thought to be important for the function of the yeast enzyme conserved. The cloned PGK cDNA could be efficiently expressed in vitro resulting in a product with the expected molecular weight.
Azevedo
J E
JE
Institut für Physiologische Chemie, Universität München.
Tropschug
M
M
Werner
S
S
D000595:Amino Acid Sequence; D001483:Base Sequence; D003062:Codon / Q000235:genetics; D004271:DNA, Fungal / Q000235:genetics; D015870:Gene Expression; D008969:Molecular Sequence Data; D009492:Neurospora crassa / Q000201:enzymology / Q000235:genetics*; D010735:Phosphoglycerate Kinase / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
D003062:Codon; D004271:DNA, Fungal; D010735:Phosphoglycerate Kinase