The healing of segmental bone defects, induced by recombinant human bone morphogenetic protein (rhBMP-2). A radiographic, histological, and biomechanical study in rats.
Subcutaneous implants of a recombinant human form of the bone-inducing protein rhBMP-2 (recombinant human bone morphogenetic protein-2) in rats have resulted in the local induction of endochondral bone formation. To test the osteoinductive activity of rhBMP-2 in an osseous location, we created five-millimeter segmental defects in the femora of forty-five adult male Sprague-Dawley rats. Two doses of lyophilized rhBMP-2 (1.4 or 11.0 micrograms) were implanted in each defect, together with guanidine-hydrochloride extracted demineralized rat-bone matrix as a carrier, and the results were compared with those in rats that had implantation of guanidine-hydrochloride extracted demineralized rat-bone matrix only. The formation and healing of bone were determined by radiographic, histological, and mechanical analysis. Both doses of rhBMP-2 induced formation of endochondral bone in the osseous defects in a dose-related manner. Implantation of 11.0 micrograms of rhBMP-2 yielded significant (p less than 0.05) bone formation, resulting in radiographic, histological, and mechanical evidence of union. Despite new-bone formation in the defects that had received 1.4 micrograms of rhBMP-2, no instances of union were observed.
Yasko
A W
AW
Research Division, Hospital for Special Surgery, Cornell University Medical Center, New York City, N.Y. 10021.
Lane
J M
JM
Fellinger
E J
EJ
Rosen
V
V
Wozney
J M
JM
Wang
E A
EA
D000818:Animals; D015519:Bone Density; D001847:Bone Diseases / Q000000981:diagnostic imaging / Q000473:pathology / Q000503:physiopathology / Q000601:surgery*; D001857:Bone Matrix; D019485:Bone Morphogenetic Proteins; D001860:Bone Plates; D002214:Capsules; D002356:Cartilage / Q000473:pathology / Q000503:physiopathology; D005269:Femur; D006133:Growth Substances / Q000008:administration & dosage / Q000494:pharmacology; D006801:Humans; D008297:Male; D009389:Neovascularization, Pathologic / Q000503:physiopathology; D010012:Osteogenesis / Q000187:drug effects / Q000502:physiology; D011095:Polyethylenes; D019736:Prostheses and Implants; D011506:Proteins / Q000008:administration & dosage / Q000494:pharmacology; D011859:Radiography; D051381:Rats; D011919:Rats, Inbred Strains; D011994:Recombinant Proteins; D013314:Stress, Mechanical; D014945:Wound Healing
D019485:Bone Morphogenetic Proteins; D002214:Capsules; D006133:Growth Substances; D011095:Polyethylenes; D011506:Proteins; D011994:Recombinant Proteins