Glycopeptide of P0 protein inhibits homophilic cell adhesion. Competition assay with transformants and peptides.
Expression of major myelin glycoprotein P0 by P0 cDNA transfection into C6 glioma cells promoted homophilic cell adhesion of the cells. After the dissociated cells were incubated for various times, the number of particles at each time point was measured. The total number of particles decreased to 24% in 60 min for transformant (C6P0) cells, in contrast to only 68% for control (C6P0') cells. To confirm the homophilic mechanism of adhesion, mixed-cell aggregation experiments were performed. Among the four synthetic peptides corresponding to a part of the P0 sequence used, only peptide 3 (residues 90-96), which contained a carbohydrate attaching site, caused considerable inhibition of cell aggregation (approximately 50%). In addition, the glycopeptide (residues 91-95) obtained from bovine P0 markedly inhibited cell aggregation (by approximately 85%).
Yazaki
T
T
Department of Physiology, Keio University School of Medicine, Tokyo, Japan.
Miura
M
M
Asou
H
H
Kitamura
K
K
Toya
S
S
Uyemura
K
K
D000595:Amino Acid Sequence; D000818:Animals; D001667:Binding, Competitive; D015153:Blotting, Western; D002448:Cell Adhesion; D015816:Cell Adhesion Molecules, Neuronal / Q000502:physiology*; D005910:Glioma; D006020:Glycopeptides / Q000502:physiology*; D007150:Immunohistochemistry; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D018993:Myelin P0 Protein; D009185:Myelin Proteins / Q000502:physiology*; D010446:Peptide Fragments / Q000502:physiology; D010957:Plasmids; D051381:Rats; D014162:Transfection; D014407:Tumor Cells, Cultured
D015816:Cell Adhesion Molecules, Neuronal; D006020:Glycopeptides; D018993:Myelin P0 Protein; D009185:Myelin Proteins; D010446:Peptide Fragments