title: Lectin cytochemistry combined with silver colloid staining of argyrophilic nucleolar organizer regions in human gliomas.
abstract: Investigation of lectin cytochemical staining of inflammatory cells in human gliomas showed that Allomyrina dichotoma (Allo-A) cytochemistry can reliably distinguish inflammatory from neoplastic cells. Allo-A cytochemistry combined with silver colloid staining of argyrophilic nucleolar organizer regions (Ag-NORs) was performed in human gliomas. Inflammatory cells possessed usually one but sometimes two Ag-NORs and macrophages often possessed several Ag-NORs. The mean Ag-NOR number per nucleus of inflammatory cells ranged from 1.81 to 2.34, and that of neoplastic cells ranged from 2.57 to 3.53 and from 2.84 to 4.46 in low- and high-grade gliomas, respectively. The mean Ag-NOR number per nucleus of inflammatory cells was significantly smaller than that of neoplastic cells (p less than 0.001). Combined Allo-A cytochemical and silver colloid Ag-NOR staining can provide a reliable Ag-NOR number in human gliomas by distinguishing inflammatory cells.
authors.affiliation: Department of Neurosurgery, Gifu University School of Medicine.
authors.forename: S
authors.initials: S
authors.lastname: Niikawa
authors.forename: A
authors.initials: A
authors.lastname: Hara
authors.forename: T
authors.initials: T
authors.lastname: Ando
authors.forename: N
authors.initials: N
authors.lastname: Sakai
authors.forename: H
authors.initials: H
authors.lastname: Yamada
authors.forename: K
authors.initials: K
authors.lastname: Shimokawa
mesh_terms: D001932:Brain Neoplasms / Q000648:ultrastructure*; D003102:Colloids; D005910:Glioma / Q000648:ultrastructure*; D006651:Histocytochemistry / Q000379:methods; D006801:Humans; D037102:Lectins; D009697:Nucleolus Organizer Region / Q000648:ultrastructure*; D012834:Silver
chemical_list: D003102:Colloids; D037102:Lectins; D012834:Silver