title: Development of potent and selective CCK-A receptor agonists from Boc-CCK-4: tetrapeptides containing Lys(N epsilon)-amide residues.
abstract: A series of Boc-CCK-4 derivatives represented by the general structure Boc-Trp-Lys(N epsilon-COR)-Asp-Phe-NH2, where R is an aromatic, heterocyclic, or aliphatic group, are potent and selective CCK-A receptor agonists. These amide-bearing compounds complement the previously described urea-based tetrapeptides (Shiosaki et al. J. Med. Chem. 1991, 34, 2837-2842); structure-activity studies revealed parallel as well as divergent trends between these two series. A significant correlation was observed between pancreatic binding affinity and the resonance constant R of the phenyl substituent in one particular series of derivatives. Sulfation of phenolic amides appended onto the epsilon-amino group of the lysine did not affect affinity for the CCK-A receptor in contrast to the 500-fold increase in binding potency observed upon sulfation of CCK-8, suggesting that the lysine appendage and the sulfated tyrosine in CCK-8, both key structural elements that impart high affinity for the CCK-A receptor, are interacting differently with the receptor. The amide-bearing tetrapeptides are full agonists relative to CCK-8 in stimulating pancreatic amylase release while being partial agonists in eliciting phosphoinositide (PI) hydrolysis. Both effects were blocked by selective CCK-A receptor antagonists.
authors.affiliation: Neuroscience Research Division, Abbott Laboratories, Abbott Park, Illinois 60064.
authors.forename: K
authors.initials: K
authors.lastname: Shiosaki
authors.forename: C W
authors.initials: CW
authors.lastname: Lin
authors.forename: H
authors.initials: H
authors.lastname: Kopecka
authors.forename: R A
authors.initials: RA
authors.lastname: Craig
authors.forename: B R
authors.initials: BR
authors.lastname: Bianchi
authors.forename: T R
authors.initials: TR
authors.lastname: Miller
authors.forename: D G
authors.initials: DG
authors.lastname: Witte
authors.forename: M
authors.initials: M
authors.lastname: Stashko
authors.forename: A M
authors.initials: AM
authors.lastname: Nadzan
mesh_terms: D000595:Amino Acid Sequence; D000681:Amylases / Q000378:metabolism; D000818:Animals; D005563:Formic Acid Esters / Q000737:chemistry* / Q000378:metabolism; D006168:Guinea Pigs; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009842:Oligopeptides / Q000138:chemical synthesis* / Q000378:metabolism / Q000494:pharmacology; D010179:Pancreas / Q000187:drug effects / Q000378:metabolism; D011949:Receptors, Cholecystokinin / Q000378:metabolism*; D012844:Sincalide / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D013329:Structure-Activity Relationship
chemical_list: D005563:Formic Acid Esters; D009842:Oligopeptides; D011949:Receptors, Cholecystokinin; C000345:t-butyloxycarbonyl group; D000681:Amylases; D012844:Sincalide