title: Monoclonal antibodies raised against pre-migratory neural crest reveal population heterogeneity during crest development.
abstract: In order to address the problem of when heterogeneity arises within premigratory and early migratory neural crest cell populations, mouse monoclonal antibodies were raised against quail premigratory neural crest. Due to the limited availability of immunogen an intrasplenic route for immunization was used. Three monoclonal antibodies (referred to as LH2D4, LH5D3 and LH6C2) were subsequently isolated which recognized subpopulations in 24 h cultures of both quail and chick mesencephalic and trunk neural crest in immunocytochemical studies. Subsequent investigations using a range of six antibodies, including LH2D4, LH5D3 and LH6C2, showed that population heterogeneity (which was not cell cycle related) could be detected as early as 15 h following mesencephalic crest explantation, a stage at which all the neural crest cells were morphologically identical. However, premigratory neural crest from the same axial level of origin was homogeneous, as judged by immunoreactivity patterns with these antibodies. Significant differences were found in the proportion of immunoreactive cells between populations of mesencephalic and trunk neural crest cultures. Double immunofluorescence studies revealed the existence of at least four separate cell populations within individual crest cultures, each identified by their unique antibody reactivity pattern, thus providing some insight into the underlying complexity of subpopulation composition within the neural crest. Immunocytochemical studies on quail embryos from stages 7-22 showed that the epitopes detected by LH2D4, LH5D3 and LH6C2 were not necessarily confined to the neural crest or to cells of crest derivation. All three epitopes displayed a spatiotemporal regulation in their expression during early avian ontogeny. Since the differential epitope expression described in this investigation was detectable as early as 15 h after premigratory neural crest explantation, took place in vitro in the absence of any other cell type and changed progressively with time, we conclude that a certain degree of population heterogeneity can be generated very early in neural crest ontogeny and independently of the tissue interactions that normally ensue in vivo.
authors.affiliation: Department of Biology, Southampton University, Bassett Crescent East, UK.
authors.forename: L
authors.initials: L
authors.lastname: Heath
authors.forename: A
authors.initials: A
authors.lastname: Wild
authors.forename: P
authors.initials: P
authors.lastname: Thorogood
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D002453:Cell Cycle; D002465:Cell Movement; D002478:Cells, Cultured; D003370:Coturnix; D004625:Embryo, Nonmammalian / Q000502:physiology; D000939:Epitopes / Q000032:analysis; D016650:Fluorescein-5-isothiocyanate; D005455:Fluorescent Antibody Technique; D005456:Fluorescent Dyes; D008636:Mesencephalon / Q000166:cytology / Q000196:embryology; D009432:Neural Crest / Q000166:cytology / Q000502:physiology*
chemical_list: D000911:Antibodies, Monoclonal; D000939:Epitopes; D005456:Fluorescent Dyes; D016650:Fluorescein-5-isothiocyanate