Studies of the influence of different cross-linking reagents on the immune response against a B-epitope.
We have previously shown that the carrier polytuftsin obtained by polycondensation of tuftsin, a naturally occurring macrophage activator, increases significantly the antibody response against a linked B-epitope. In the present work, we have studied the influence of different cross-linking reagents on the quality of the conjugation and on the immune response, at both B-cell and T-cell levels. We observed that the cross-linking method used for coupling the B-epitope to the carrier influences the immune response. A hypothesis is put forward to explain the differences observed.
Delmas
A
A
Centre de Biophysique Moléculaire, Orléans, France.
Brack
A
A
Trudelle
Y
Y
D000595:Amino Acid Sequence; D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D003432:Cross-Linking Reagents / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology; D006510:Hepatitis B Antibodies / Q000096:biosynthesis; D006514:Hepatitis B Surface Antigens / Q000276:immunology*; D008301:Maleimides / Q000737:chemistry*; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D011108:Polymers / Q000737:chemistry*; D011498:Protein Precursors / Q000276:immunology*; D013388:Succinimides / Q000737:chemistry*; D013440:Sulfides / Q000737:chemistry*; D013601:T-Lymphocytes / Q000276:immunology; D014405:Tuftsin / Q000737:chemistry*
D003432:Cross-Linking Reagents; D000939:Epitopes; D006510:Hepatitis B Antibodies; D006514:Hepatitis B Surface Antigens; D008301:Maleimides; D011108:Polymers; D011498:Protein Precursors; D013388:Succinimides; D013440:Sulfides; C050807:presurface protein 2, hepatitis B surface antigen; C075481:succinimidyl 3-N-maleimidopropionate; C053968:polytuftsin; C039011:N-hydroxysuccinimide S-acetylthioacetate; D014405:Tuftsin