A PCR-based method of identifying species-specific repeated DNAs.
A PCR-based method is described to facilitate the identification of DNA fragments that are highly repeated and species-specific. The precision of the technique was demonstrated by cloning a fragment that occurred in a high number of copies in the plant species Medicago granadensis but in a low number of copies in 17 other Medicago species and Melilotus officinalis. This method should greatly accelerate the isolation and cloning of short and long interspersed nuclear elements with species-specific distributions. Such clones should prove useful in studies of phylogenetic relationships in the identification of interspecific hybrids, as in situ chromosome markers and other applications.
Skinner
D Z
DZ
USDA-ARS, Dept. of Agronomy, Kansas State University, Manhattan 66506-5501.
D001483:Base Sequence; D004247:DNA / Q000032:analysis* / Q000235:genetics; D000455:Medicago sativa / Q000235:genetics; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012091:Repetitive Sequences, Nucleic Acid; D013045:Species Specificity
D004247:DNA