Cloning of the rat insulin-like growth factor binding protein-1 gene and analysis of its 5' promoter region.
To understand specific mechanisms involved in the regulation of insulin-like growth factor binding protein-1 (IGFBP-1), an important modulator of IGF bioactivity, we cloned the rat IGFBP-1 gene and sequenced a 1.5 kb Sph1-Sph1 fragment containing 1110 bases upstream from the translation start site. Computer analysis reveals the presence of ATA, CACCC, and CCAAT elements, and putative homeodomain, AP-1, insulin and glucocorticoid response elements in the 5' promoter. Primer extension and ribonuclease protection studies reveal a single cap site in RNA from rat hepatoma cells and both control and diabetic rat liver.
Unterman
T G
TG
Department of Internal Medicine, University of Illinois College of Medicine, Chicago.
Lacson
R G
RG
McGary
E
E
Whalen
C
C
Purple
C
C
Goswami
R G
RG
D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D005796:Genes; D005801:Genes, Homeobox; D005809:Genes, Regulator; D018970:Insulin-Like Growth Factor Binding Protein 1; D008099:Liver / Q000378:metabolism; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D011401:Promoter Regions, Genetic; D051381:Rats; D013002:Somatomedins / Q000378:metabolism
D002352:Carrier Proteins; D018970:Insulin-Like Growth Factor Binding Protein 1; D009838:Oligodeoxyribonucleotides; D013002:Somatomedins; D004247:DNA