title: Ion channels from the Bacillus subtilis plasma membrane incorporated into planar lipid bilayers.
abstract: Fusion of Bacillus subtilis plasma membrane vesicles with planar lipid bilayers induced the appearance of discrete current fluctuations characteristic of ion channels. These channels showed a wide range of conductances and kinetic behaviors. In 300 mM KCl, their conductances ranged from a few hundreds of pS to more than 1 nS, and most of them exhibited several sub-states. The channels poorly discriminated between small univalent anions and cations. Some of them showed voltage dependence and most of them presented a complex gating kinetics. The results are consistent with the hypothesis of the presence in the B. subtilis plasma membrane of pores composed of subunits that function cooperatively.
authors.affiliation: Departamento de Biología, Facultad de Ciencias, Universidad de Chile, Santiago.
authors.forename: C
authors.initials: C
authors.lastname: Alcayaga
authors.forename: R
authors.initials: R
authors.lastname: Venegas
authors.forename: A
authors.initials: A
authors.lastname: Carrasco
authors.forename: D
authors.initials: D
authors.lastname: Wolff
mesh_terms: D001412:Bacillus subtilis / Q000502:physiology*; D002462:Cell Membrane / Q000502:physiology*; D004553:Electric Conductivity / Q000187:drug effects; D007473:Ion Channels / Q000187:drug effects / Q000502:physiology*; D007700:Kinetics; D008051:Lipid Bilayers; D008561:Membrane Fusion; D008564:Membrane Potentials / Q000187:drug effects; D010714:Phosphatidylethanolamines / Q000494:pharmacology; D011189:Potassium Chloride / Q000494:pharmacology
chemical_list: D007473:Ion Channels; D008051:Lipid Bilayers; D010714:Phosphatidylethanolamines; D011189:Potassium Chloride