Identification of a repetitive element in the snail Biomphalaria glabrata: relationship to the reverse transcriptase-encoding sequence in LINE-1 transposons.
BamHI-digested Biomphalaria glabrata DNA contains a repetitive 2.0-kb fragment which is readily discernible by ethidium bromide staining. We present evidence that this repetitive element is related at both the nucleotide and amino acid levels to long interspersed nuclear element (LINE)-like transposons. Although comparable elements have been described in several invertebrates, this is the first report of a molluscan homologue. In common with LINE transposons, an open reading frame in the B. glabrata element shows significant homology to reverse transcriptase--a feature believed to allow the dissemination of these elements in the eukaryotic genome.
Knight
M
M
Biomedical Research Institute, Rockville, MD 20852.
Miller
A
A
Raghavan
N
N
Richards
C
C
Lewis
F
F
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001700:Biomphalaria / Q000235:genetics*; D004251:DNA Transposable Elements / Q000235:genetics*; D015245:Deoxyribonuclease BamHI; D015698:Genomic Library; D008969:Molecular Sequence Data; D012194:RNA-Directed DNA Polymerase / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
D004251:DNA Transposable Elements; D012194:RNA-Directed DNA Polymerase; D015245:Deoxyribonuclease BamHI