Broad cross-reactivity with marked fine specificity in the cytotoxic T cell response to flaviviruses.
Cytotoxic T (Tc) cells were generated in mice of five H-2 haplotypes against the flaviviruses Kunjin and West Nile (WNV). A panel of recombinant vaccinia viruses which between them expressed cDNA of the entire Kunjin virus genome were used to infect targets. Anti-Kunjin virus responses to determinants derived from non-structural proteins, especially NS3, NS4A and NS4B, were dominant in most mouse strains; usually only one class I major histocompatibility complex (MHC) restriction element was involved. WNV-immune Tc cells showed similar but not identical patterns of antigen recognition to Kunjin virus-immune Tc cells. The extent to which WNV-immune Tc cells recognized Kunjin virus-encoded determinants varied considerably between mice of different MHC haplotypes.
Hill
A B
AB
Division of Cell Biology, John Curtin School of Medical Research, Australian National University, Canberra, ACT.
Müllbacher
A
A
Parrish
C
C
Coia
G
G
Westaway
E G
EG
Blanden
R V
RV
D000818:Animals; D001483:Base Sequence; D003429:Cross Reactions; D003602:Cytotoxicity, Immunologic / Q000276:immunology; D004274:DNA, Recombinant; D000939:Epitopes / Q000276:immunology*; D005416:Flavivirus / Q000276:immunology*; D016679:Genome, Viral; D006239:Haplotypes; D016056:Immunodominant Epitopes / Q000276:immunology; D007156:Immunologic Memory / Q000276:immunology; D008285:Major Histocompatibility Complex / Q000276:immunology; D051379:Mice; D008969:Molecular Sequence Data; D013047:Specific Pathogen-Free Organisms; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014616:Vaccinia virus / Q000276:immunology; D014902:West Nile virus / Q000276:immunology
D004274:DNA, Recombinant; D000939:Epitopes; D016056:Immunodominant Epitopes