Re-investigation of the structure of the capsular polysaccharide of Klebsiella K15 using bacteriophage degradation and inverse-detected NMR experiments.
The structure of the capsular polysaccharide from Klebsiella K15 has been re-investigated, principally by 1D and 2D 1H- and 13C-NMR spectroscopy of the oligosaccharide-alditol obtained by depolymerisation of the polysaccharide with a viral-borne endoglycanase followed by borohydride reduction of the isolated repeating oligosaccharide. The capsular polysaccharide was shown to have the repeating unit: [Formula: see text]
Parolis
H
H
School of Pharmaceutical Sciences, Rhodes University, Grahamstown, South Africa.
Parolis
L A
LA
Whittaker
D V
DV
D001435:Bacteriophages / Q000201:enzymology*; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D006026:Glycoside Hydrolases / Q000378:metabolism*; D007202:Indicators and Reagents; D007709:Klebsiella / Q000737:chemistry* / Q000201:enzymology; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008745:Methylation; D008969:Molecular Sequence Data; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism
D007202:Indicators and Reagents; D011135:Polysaccharides, Bacterial; D006026:Glycoside Hydrolases