[Preparation of liposomes entrapping plasmid DNA and linear DNA].
Liposomes entrapping plasmids pSV2-neo DNA, pUC18-ras DNA, pSV2-neo-ras DNA and linear DNA were prepared. The liposomes were composed of DOPC/Chol/OA (4:4:3) and pH-sensitive DOPE/Chol/OA (4:4:3), respectively. The efficiency of DNA entrapment was about 50%. Gel electrophoresis analysis showed: Liposome-entrapped DNA was not digested by DNase; The entrapped DNA molecules were intact and stable for at least 5-6 months at 4 degrees C. During preparation of pH-sensitive liposome, the pH must be kept at 8.0.
Liu
Q
Q
Institute of Oncology, Beijing.
D004247:DNA / Q000008:administration & dosage*; D008081:Liposomes; D010957:Plasmids
D008081:Liposomes; D004247:DNA