title: Lymphocyte immunophenotyping by flow cytometry in normal adults. Comparison of fresh whole blood lysis technique, Ficoll-Paque separation and cryopreservation.
abstract: In the present report we have assessed the extent to which Ficoll-Paque separation and cryopreservation of mononuclear cells alter the measurement of lymphocyte subsets by flow cytometry. Standard Ficoll-Paque separation increased the percentage of CD4+, CD19+ and CD4+CD45RA+ cells, as well as decreasing that of CD8+, and CD4+CD29+ cells, compared to the fresh whole blood lysis technique. Moreover, cryopreservation caused a depletion of CD4+ p80+ cells, but normal whole blood values were restored following a short incubation.
authors.affiliation: Servei d'Immunologia, Hospital Duran i Reynals, Barcelona, Catalunya, Spain.
authors.forename: M A
authors.initials: MA
authors.lastname: Romeu
authors.forename: M
authors.initials: M
authors.lastname: Mestre
authors.forename: L
authors.initials: L
authors.lastname: González
authors.forename: A
authors.initials: A
authors.lastname: Valls
authors.forename: J
authors.initials: J
authors.lastname: Verdaguer
authors.forename: M
authors.initials: M
authors.lastname: Corominas
authors.forename: J
authors.initials: J
authors.lastname: Bas
authors.forename: E
authors.initials: E
authors.lastname: Massip
authors.forename: E
authors.initials: E
authors.lastname: Buendia
mesh_terms: D000328:Adult; D015703:Antigens, CD / Q000032:analysis; D015925:Cryopreservation; D005260:Female; D005362:Ficoll / Q000009:adverse effects; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D016130:Immunophenotyping / Q000379:methods*; D008214:Lymphocytes / Q000276:immunology*; D008297:Male; D012680:Sensitivity and Specificity
chemical_list: D015703:Antigens, CD; D005362:Ficoll