title: High affinity for class II molecules as a necessary but not sufficient characteristic of encephalitogenic determinants.
abstract: A direct binding assay specific for IAs molecules has been developed and its immunological relevance validated by examining, for a panel of nine different synthetic peptides, the correlation between their capacity to bind purified IAs and to inhibit IAs-restricted antigen presentation. The IAs assay thus developed has then been used to study the IAs binding affinity of a set of overlapping peptides spanning the entire myelin basic protein (MBP). It was found that the encephalitogenic MBP region corresponds to peptides with high MHC binding affinities. Other regions of the MBP that have not been described as being pathogenic in the context of IAs molecules have also been found to be high IAs binders, suggesting that variables other than MHC affinity are also involved in determining the pathogenic potential of self-derived determinants.
authors.affiliation: Cytel, San Diego, CA 92121.
authors.forename: M
authors.initials: M
authors.lastname: Wall
authors.forename: S
authors.initials: S
authors.lastname: Southwood
authors.forename: J
authors.initials: J
authors.lastname: Sidney
authors.forename: C
authors.initials: C
authors.lastname: Oseroff
authors.forename: M F
authors.initials: MF
authors.lastname: del Guericio
authors.forename: A G
authors.initials: AG
authors.lastname: Lamont
authors.forename: S M
authors.initials: SM
authors.lastname: Colón
authors.forename: T
authors.initials: T
authors.lastname: Arrhenius
authors.forename: F C
authors.initials: FC
authors.lastname: Gaeta
authors.forename: A
authors.initials: A
authors.lastname: Sette
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001324:Autoantigens / Q000378:metabolism; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000209:etiology* / Q000276:immunology; D000939:Epitopes / Q000378:metabolism; D000949:Histocompatibility Antigens Class II / Q000378:metabolism*; D066298:In Vitro Techniques; D007700:Kinetics; D051379:Mice; D008969:Molecular Sequence Data; D004676:Myelin Basic Protein / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism; D010455:Peptides / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D011485:Protein Binding
chemical_list: D001324:Autoantigens; D000939:Epitopes; D000949:Histocompatibility Antigens Class II; D004676:Myelin Basic Protein; D010455:Peptides