Rapid and simple method for the identification of apolipoprotein E isomorphic phenotypes from whole serum.
For the identification of apolipoprotein E isomorphic phenotypes, fresh or thawed serum was analyzed without prior delipidation or other pretreatment. Using 5% polyacrylamide gels with a 40 mm interelectrode distance, the isoforms were separated by isoelectric focusing in immobilized pH gradients ranging from pH 5 to 6.5, and transferred onto polyvinylidene difluoride membranes by contact blotting for 1 h. The apolipoprotein E isoforms were identified following immunostaining. The electrophoresis required less than 2 h and the entire procedure could be completed within 6 h.
Kohlmeier
M
M
Universitätsklinikum Rudolf Virchow, Freien Universität, Institut für Klinische Chemie und Biochemie, Berline, Germany.
Drossel
H J
HJ
Sinha
P
P
Köttgen
E
E
D000911:Antibodies, Monoclonal / Q000302:isolation & purification; D001057:Apolipoproteins E / Q000097:blood / Q000235:genetics*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D015151:Immunoblotting; D007525:Isoelectric Focusing; D010641:Phenotype; D013194:Staining and Labeling; D013997:Time Factors
D000911:Antibodies, Monoclonal; D001057:Apolipoproteins E