title: Amino acids and peptides. XXXIII. Synthesis of N-terminal epitope peptides of mammalian metallothioneins (MTs).
abstract: In order to determine the fine structure of the mammalian metallothionein (MT) epitope to a monoclonal anti-rat Zn-MT-II antibody (MT 189-14-7), N-terminal peptides of various lengths of mammalian metallothioneins (MTs) were synthesized by a conventional solution method using the newly developed beta-2-adamantylaspartate, and their immunological properties were examined. It was found that the N-terminal acetyl group was indispensable for the reaction with the monoclonal antibody and the N-terminally acetylated pentapeptide, Ac-Met-Asp-Pro-Asn-Cys-OH, was the smallest peptide which exhibited a significant reactivity with the antibody.
authors.affiliation: Faculty of Pharmaceutical Sciences, Kobe-Gakuin University, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Okada
authors.forename: S
authors.initials: S
authors.lastname: Nakayama
authors.forename: S
authors.initials: S
authors.lastname: Iguchi
authors.forename: Y
authors.initials: Y
authors.lastname: Kikuchi
authors.forename: M
authors.initials: M
authors.lastname: Irie
authors.forename: J
authors.initials: J
authors.lastname: Sawada
authors.forename: H
authors.initials: H
authors.lastname: Ikebuchi
authors.forename: T
authors.initials: T
authors.lastname: Terao
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000138:chemical synthesis*; D000818:Animals; D000939:Epitopes; D008668:Metallothionein / Q000138:chemical synthesis / Q000276:immunology*; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000138:chemical synthesis* / Q000276:immunology
chemical_list: D000596:Amino Acids; D000939:Epitopes; D010446:Peptide Fragments; D008668:Metallothionein