Immunodominant structures in the aminoterminal portion of human interferon alpha 1.
The analysis of the antigenic structure of human interferon (IFN)-alpha 1 with a panel of monoclonal antibodies revealed four immunodominant regions. Three of them, recognized by 12 of 14 antibodies were mapped into the aminoterminal portion of IFN-alpha 1 around residues 31-38, 43-53 and 63-85. The region 31-85 proved important also for the antiviral and antiproliferative activity of the IFN-alpha 1 molecule. The antibody recognizing the sequence around residues (54)63-67 also inhibited the cellular binding of IFN-alpha 1 to the high-affinity receptors.
Kontsek
P
P
Institute of Virology, Slovak Academy of Sciences, Bratislava.
Borecký
L
L
Kontseková
E
E
Kolcunová
A
A
Novák
M
M
Zav'yalov
V P
VP
Maiorov
V A
VA
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000276:immunology; D000937:Antigen-Antibody Reactions; D001667:Binding, Competitive; D002455:Cell Division; D000939:Epitopes; D006801:Humans; D016898:Interferon-alpha / Q000737:chemistry / Q000276:immunology*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D010455:Peptides / Q000737:chemistry / Q000276:immunology; D011487:Protein Conformation; D011971:Receptors, Immunologic / Q000378:metabolism; D017471:Receptors, Interferon; D013329:Structure-Activity Relationship
D000911:Antibodies, Monoclonal; D000939:Epitopes; D016898:Interferon-alpha; D010455:Peptides; D011971:Receptors, Immunologic; D017471:Receptors, Interferon