Acetylcholine receptor channel structure probed in cysteine-substitution mutants.
In order to understand the structural bases of ion conduction, ion selectivity, and gating in the nicotinic acetylcholine receptor, mutagenesis and covalent modification were combined to identify the amino acid residues that line the channel. The side chains of alternate residues--Ser248, Leu250, Ser252, and Thr254--in M2, a membrane-spanning segment of the alpha subunit, are exposed in the closed channel. Thus alpha 248-254 probably forms a beta strand, and the gate is closer to the cytoplasmic end of the channel than any of these residues. On channel opening, Leu251 is also exposed. These results lead to a revised view of the closed and open channel structures.
Akabas
M H
MH
Department of Physiology, College of Physicians and Surgeons, Columbia University, New York, NY 10032.
Stauffer
D A
DA
Xu
M
M
Karlin
A
A
D000109:Acetylcholine / Q000378:metabolism / Q000494:pharmacology; D000595:Amino Acid Sequence; D000818:Animals; D003545:Cysteine / Q000737:chemistry*; D015870:Gene Expression; D015640:Ion Channel Gating; D007473:Ion Channels / Q000737:chemistry* / Q000502:physiology; D051379:Mice; D008969:Molecular Sequence Data; D009132:Muscles / Q000737:chemistry; D016296:Mutagenesis; D009865:Oocytes / Q000378:metabolism; D011950:Receptors, Cholinergic / Q000737:chemistry* / Q000235:genetics; D013329:Structure-Activity Relationship; D013439:Sulfhydryl Reagents / Q000494:pharmacology; D013816:Thermodynamics; D014162:Transfection; D014981:Xenopus
D007473:Ion Channels; D011950:Receptors, Cholinergic; D013439:Sulfhydryl Reagents; D003545:Cysteine; D000109:Acetylcholine