Identification of basic nuclear proteins by their boronate complex.
A new histochemical reaction for the identification of histone type basic proteins has been developed. Carbonyldiimidazol is used to activate the basic proteins of TCA-extracted nuclei, their m-aminophenylboronic acid complex is prepared, and the DNA-free, histone-containing nuclei are stained with toluidine blue at pH 5.5.
Jobst
K
K
Department of Clinical Chemistry, University School of Medicine, Pécs, Hungary.
Lakatos
A
A
Horváth
A
A
D000818:Animals; D001895:Boron; D002467:Cell Nucleus / Q000737:chemistry*; D006651:Histocytochemistry / Q000379:methods*; D006657:Histones / Q000032:analysis*; D007093:Imidazoles; D051379:Mice; D009687:Nuclear Proteins / Q000032:analysis*; D016612:Paraffin Embedding; D013194:Staining and Labeling; D014048:Tolonium Chloride
D006657:Histones; D007093:Imidazoles; D009687:Nuclear Proteins; D014048:Tolonium Chloride; C006900:N,N-carbonyldiimidazole; D001895:Boron