Activation of human CD4+CD45RA+ T cells by chrysotile asbestos in vitro.
Chrysotile asbestos stimulates T lymphocyte subsets. Cell surface CD4 or CD45RA expression in peripheral blood mononuclear cells (PBMC) was downregulated after incubation with chrysotile asbestos in vitro temporarily. The percentage of CD4+CD45RA+ cells and mean fluorescence intensity in CD4 or CD45RA decreased after incubation with asbestos and returned to the original level after 24 h of incubation, which suggests that chrysotile asbestos activates CD4+CD45RA+ cells. No change was observed in CD29 expression. An increased percentage of IL-2R positive cells and an elevated intracellular Ca++ level were also indicative of the activation of PBMC by chrysotile asbestos.
Kinugawa
K
K
Department of Hygiene, Kawasaki Medical School, Kurashiki, Japan.
Ueki
A
A
Yamaguchi
M
M
Watanabe
Y
Y
Kawakami
Y
Y
Hyodoh
F
F
Tsushima
H
H
D015703:Antigens, CD / Q000032:analysis; D001194:Asbestos / Q000633:toxicity*; D017632:Asbestos, Serpentine; D015704:CD4 Antigens / Q000032:analysis*; D016827:CD8 Antigens / Q000032:analysis; D002118:Calcium / Q000032:analysis; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D006801:Humans; D019012:Integrin beta1; D017493:Leukocyte Common Antigens / Q000032:analysis*; D008213:Lymphocyte Activation / Q000187:drug effects*; D015375:Receptors, Interleukin-2 / Q000032:analysis; D013601:T-Lymphocytes / Q000187:drug effects* / Q000276:immunology
D015703:Antigens, CD; D017632:Asbestos, Serpentine; D015704:CD4 Antigens; D016827:CD8 Antigens; D019012:Integrin beta1; D015375:Receptors, Interleukin-2; D001194:Asbestos; D017493:Leukocyte Common Antigens; D002118:Calcium