Application of the Tradescantia micronucleus assay for the genetic evaluation of chemical mixtures in soil and aqueous media.
Genotoxic evaluations of arsenic trioxide, dieldrin, lead tetraacetate and their nine binary and one tertiary mixtures were performed using the Tradescantia micronucleus (Trad-MN) assay. The chemicals or their mixtures were either (1) mixed into soil, and chemical exposure to the target cells was through the roots of intact plants grown in the soil or (2) through plant cuttings in which the inflorescences received treatment by absorption through stem of an aqueous solution of the test chemicals. All three chemicals yielded clastogenic responses when tested in soil medium and only two of these i.e. arsenic trioxide and dieldrin were positive when plant cuttings were exposed to the test chemicals in the aqueous medium. The clastogenicity of the chemical mixtures was modified by the ratio of the individual chemical in a particular mixture and also by the medium in which these mixtures were tested.
Gill
B S
BS
Environmental Health Research and Testing, Research Triangle Park, NC 27709.
Sandhu
S S
SS
D001151:Arsenic / Q000494:pharmacology*; D000077237:Arsenic Trioxide; D001152:Arsenicals; D004026:Dieldrin / Q000494:pharmacology*; D015162:Micronucleus Tests / Q000379:methods*; D009153:Mutagens / Q000032:analysis* / Q000494:pharmacology; D009942:Organometallic Compounds / Q000494:pharmacology*; D010087:Oxides; D010944:Plants / Q000187:drug effects* / Q000235:genetics; D012989:Soil Pollutants / Q000032:analysis*; D014874:Water Pollutants, Chemical / Q000032:analysis*
D001152:Arsenicals; D009153:Mutagens; D009942:Organometallic Compounds; D010087:Oxides; D012989:Soil Pollutants; D014874:Water Pollutants, Chemical; C029433:lead tetraacetate; D004026:Dieldrin; D001151:Arsenic; D000077237:Arsenic Trioxide