title: The expression of leukocyte-endothelial adhesion molecules is increased in perennial allergic rhinitis.
abstract: Accumulating evidence supports the importance of leukocyte-endothelial cell adhesion molecule (CAM) expression as an initiating process in tissue inflammation. To investigate the relevance of CAM expression to allergic airways inflammation, nasal biopsies from patients with perennial allergic rhinitis (n = 8) and from nonatopic healthy volunteers (n = 8) were immunostained with monoclonal antibodies directed against the CAMs, intercellular adhesion molecule-1 (ICAM-1), endothelial cell adhesion molecule-1 (ELAM-1), and vascular cell adhesion molecule-1 (VCAM-1). The endothelial staining of these CAMs was related to the number of vessels within each biopsy, delineated by a monoclonal antibody against Ulex europaeus-1 lectin bound to endothelial cells, and to the number of tissue leukocytes staining for one of the ligands of ICAM-1, the beta 2 integrin, lymphocyte function-associated antigen (LFA-1). Expression of CAMs was related to the number of infiltrating neutrophils, eosinophils, and lymphocytes identified immunohistochemically within the biopsies. ICAM-1 was the most prominent CAM present on the endothelium of the normal nasal mucosa, with less expression of ELAM-1 and only minimal or absent expression of VCAM-1. In perennial rhinitis, both ICAM-1 (P less than 0.05) and VCAM-1 (P less than 0.01) expression on endothelial cells were increased and were positively correlated in their level of expression (P less than 0.002). The number of tissue LFA-1-positive cells was significantly greater (P less than 0.05) in the biopsies from the perennial rhinitics (median, 27.3/mm2) than from the healthy controls (median, 5.3 cells/mm2). LFA-1 expression significantly correlated with the number of ICAM-1-positive vessels (P less than 0.03).(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Immunopharmacology Group, Southampton General Hospital, United Kingdom.
authors.forename: S
authors.initials: S
authors.lastname: Montefort
authors.forename: I H
authors.initials: IH
authors.lastname: Feather
authors.forename: S J
authors.initials: SJ
authors.lastname: Wilson
authors.forename: D O
authors.initials: DO
authors.lastname: Haskard
authors.forename: T H
authors.initials: TH
authors.lastname: Lee
authors.forename: S T
authors.initials: ST
authors.lastname: Holgate
authors.forename: P H
authors.initials: PH
authors.lastname: Howarth
mesh_terms: D000328:Adult; D000911:Antibodies, Monoclonal; D001706:Biopsy; D015815:Cell Adhesion Molecules / Q000032:analysis / Q000096:biosynthesis*; D019040:E-Selectin; D005260:Female; D006801:Humans; D007249:Inflammation; D018799:Intercellular Adhesion Molecule-1; D016169:Lymphocyte Function-Associated Antigen-1 / Q000032:analysis / Q000096:biosynthesis; D008297:Male; D009297:Nasal Mucosa / Q000473:pathology / Q000502:physiology / Q000503:physiopathology*; D012016:Reference Values; D012221:Rhinitis, Allergic, Perennial / Q000473:pathology / Q000503:physiopathology*; D019010:Vascular Cell Adhesion Molecule-1
chemical_list: D000911:Antibodies, Monoclonal; D015815:Cell Adhesion Molecules; D019040:E-Selectin; D016169:Lymphocyte Function-Associated Antigen-1; D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1