title: Immunohistological localization of cell adhesion proteins and integrins in the periodontium.
abstract: The distribution of the cell adhesion proteins vitronectin, fibronectin, tenascin, and laminin as well as several integrin subunits, alpha 2, alpha 5, and alpha v, was studied in primate periodontal tissues. Full baboon mandibular sections were analyzed by immunohistochemical methods in order to localize the molecules studied in both soft and hard tissues. Vitronectin was associated with the connective tissue of the marginal gingiva, the periodontal ligament, as well as the endosteum and periosteum. A notable finding was the particularly high staining intensity of vitronectin in the periodontal ligament. Fibronectin was widely distributed in the periodontal connective tissue and was also localized to the pericellular matrix of osteocytes and blood vascular elements. Epithelial basement membranes stained positively for both fibronectin and tenascin. These proteins were also expressed in the periosteal and endosteal connective tissues and the periodontal ligament. The staining intensity for tenascin was higher in zones along the cementum and bone surfaces. Laminin was, characteristically, limited to basement membranes of epithelium and endothelium. The distribution of fibronectin, tenascin, and laminin is related to previous findings in other species. The localization of the several integrin alpha-subunits is also described in full baboon mandibular sections. The vitronectin receptor (alpha v) had a uniquely strong expression in osteoclasts of the alveolar bone and was found, at lesser intensity, on periodontal ligament fibroblasts. The fibronectin receptor alpha subunit, alpha 5, was also observed on osteoclasts, and, in addition, was widely distributed on fibroblasts, cementoblasts, and osteoblasts.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Periodontics, UTHSC, San Antonio, TX.
authors.forename: B
authors.initials: B
authors.lastname: Steffensen
authors.forename: A H
authors.initials: AH
authors.lastname: Duong
authors.forename: S B
authors.initials: SB
authors.lastname: Milam
authors.forename: C L
authors.initials: CL
authors.lastname: Potempa
authors.forename: W B
authors.initials: WB
authors.lastname: Winborn
authors.forename: V L
authors.initials: VL
authors.lastname: Magnuson
authors.forename: D
authors.initials: D
authors.lastname: Chen
authors.forename: G
authors.initials: G
authors.lastname: Zardeneta
authors.forename: R J
authors.initials: RJ
authors.lastname: Klebe
mesh_terms: D000539:Alveolar Process / Q000737:chemistry / Q000648:ultrastructure; D000818:Animals; D002352:Carrier Proteins / Q000032:analysis; D015815:Cell Adhesion Molecules / Q000032:analysis*; D015816:Cell Adhesion Molecules, Neuronal / Q000032:analysis; D003094:Collagen / Q000032:analysis; D003238:Connective Tissue / Q000737:chemistry / Q000648:ultrastructure; D003739:Dental Cementum / Q000737:chemistry / Q000648:ultrastructure; D016326:Extracellular Matrix Proteins / Q000032:analysis; D005260:Female; D005353:Fibronectins / Q000032:analysis; D005881:Gingiva / Q000737:chemistry / Q000648:ultrastructure; D006023:Glycoproteins / Q000032:analysis; D007150:Immunohistochemistry; D016023:Integrins / Q000032:analysis*; D007797:Laminin / Q000032:analysis; D010215:Papio; D010513:Periodontal Ligament / Q000737:chemistry / Q000648:ultrastructure; D010519:Periodontium / Q000737:chemistry / Q000648:ultrastructure*; D010521:Periosteum / Q000737:chemistry / Q000648:ultrastructure; D011956:Receptors, Cell Surface / Q000032:analysis; D038981:Receptors, Collagen; D017469:Receptors, Fibronectin; D011971:Receptors, Immunologic / Q000032:analysis; D019030:Receptors, Vitronectin; D019063:Tenascin; D019096:Vitronectin
chemical_list: D002352:Carrier Proteins; D015815:Cell Adhesion Molecules; D015816:Cell Adhesion Molecules, Neuronal; D016326:Extracellular Matrix Proteins; D005353:Fibronectins; D006023:Glycoproteins; D016023:Integrins; D007797:Laminin; D011956:Receptors, Cell Surface; D038981:Receptors, Collagen; D017469:Receptors, Fibronectin; D011971:Receptors, Immunologic; D019030:Receptors, Vitronectin; D019063:Tenascin; D019096:Vitronectin; D003094:Collagen