title: Immunogenic tumor variants induced by drug treatment of the L5178Y lymphoma: search for serologically defined antigens at the clonal level.
abstract: Highly immunogenic tumor variants are generated by in vitro or in vivo treatment of the murine L5178Y lymphoma line with triazene derivatives. Most of these variants express new transplantation- and antibody-defined antigens that previous studies have shown to be closely related. One such 80-kDa protein on the surface of clone-D cells was found to be related to xenotropic MuLV gp70 molecules. To investigate the possible relevance of clone-D data to general properties of immunogenic variants in this tumor model system, polyclonal syngeneic antisera raised to a panel of immunogenic clones (including clone D) of the drug-treated L5178Y lymphoma line were employed in the immunoprecipitation of cell-surface and intrinsically labeled variant cells. In all clones, 1- and 2-dimensional electrophoretic analysis of the immunoprecipitates detected an antigen of approximately 80 kDa, and 35S-labeled 80-kDa molecules could be cross-precipitated from all clones by the panel of clone-specific antisera. In addition, 45- and 30-kDa components were also found in metabolically labeled variant cells. While the surface 80-kDa component was reactive with anti-xenotropic gp70 antibodies, the 30-kDa molecule was removed by anti-gag p30 antibody in sequential immunoprecipitation experiments. These data suggest that expression of aberrant, retrovirus-related proteins is a common finding in immunogenic cells of the drug-treated L5178Y lymphoma line.
authors.affiliation: Department of Experimental Medicine and Biochemical Sciences, University of Perugia, Italy.
authors.forename: U
authors.initials: U
authors.lastname: Grohmann
authors.forename: P
authors.initials: P
authors.lastname: Puccetti
authors.forename: L
authors.initials: L
authors.lastname: Romani
authors.forename: L
authors.initials: L
authors.lastname: Binaglia
authors.forename: R
authors.initials: R
authors.lastname: Bianchi
authors.forename: M L
authors.initials: ML
authors.lastname: Belladonna
authors.forename: S J
authors.initials: SJ
authors.lastname: Ullrich
authors.forename: E
authors.initials: E
authors.lastname: Appella
authors.forename: M C
authors.initials: MC
authors.lastname: Fioretti
mesh_terms: D000818:Animals; D000951:Antigens, Neoplasm / Q000032:analysis* / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D008223:Lymphoma / Q000188:drug therapy* / Q000276:immunology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008811:Mice, Inbred DBA; D011233:Precipitin Tests; D012191:Retroviridae Proteins / Q000032:analysis / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology
chemical_list: D000951:Antigens, Neoplasm; D012191:Retroviridae Proteins