Western ligand blot assay for human growth hormone-dependent insulin-like growth factor binding protein (IGFBP-3): the serum levels in patients with classical growth hormone deficiency.
The insulin-like growth factors I and II (IGFs), important growth factors both in vivo and in vitro, are known to have at least six binding proteins (IGFBP-1-6). In human serum, IGFBP-3 is a major binding protein and is considered to be GH-IGF-I-dependent. We have established a Western Ligand Blot (WLB) assay for IGFBP-3. The method is a densitometric analysis of IGFBP-3 bands on a film of WLB. The IGFBP-3 levels of patients with classical growth hormone deficiency (GHD, 5 isolated and 10 multiple hormone deficiencies with appropriate therapy) were studied. Before puberty there is no overlap between control (n = 31) and the patients with GHD (n = 10). However, IGFBP-3 levels of two of five pubertal patients with GHD were within the normal range (n = 16). We think that measurement of serum IGFBP-3 is a useful diagnostic marker for GHD, especially before puberty.
Hasegawa
Y
Y
Division of Endocrinology and Metabolism, Tokyo Metropolitan Kiyose Children's Hospital, Japan.
Hasegawa
T
T
Yokoyama
T
T
Kotoh
S
S
Tsuchiya
Y
Y
Kurimoto
F
F
D000293:Adolescent; D000328:Adult; D015153:Blotting, Western; D002352:Carrier Proteins / Q000097:blood*; D002648:Child; D002675:Child, Preschool; D003720:Densitometry; D005260:Female; D013006:Growth Hormone / Q000172:deficiency* / Q000502:physiology; D006801:Humans; D018969:Insulin-Like Growth Factor Binding Proteins; D008297:Male; D011627:Puberty / Q000097:blood; D012016:Reference Values; D013002:Somatomedins
D002352:Carrier Proteins; D018969:Insulin-Like Growth Factor Binding Proteins; D013002:Somatomedins; D013006:Growth Hormone