[Determination of N'methylnicotinamide and nicotine coenzymes in biological in biological media by the fluorescent method].
Assay of N1-MNA was conducted in 96 urine samples using two methodological variants with external and internal N1-MNA standards. Basing on significant fluctuations of the percent of detecting N1-MNA added to urine, the necessity of using the internal standard was proved. A significant (up to 30%) overestimating of the values in using the external standard makes difficult revealing niacin deficiency. It has been recommended that NAD preparation be used as an internal standard in NAD + NADP assay in the blood, that permits one to simplify significantly the counting and to avoid the universal coefficient (used in literature) of recalculating N1-MNA fluorescence to nicotinamide coenzyme fluorescence because this coefficient is not a constant value.
Kodentsova
V M
VM
Vrzhesinskaia
O A
OA
Sokol'nikov
A A
AA
Zaburkina
T G
TG
Bender
E D
ED
Spirichev
V B
VB
D000818:Animals; D001923:Brain Chemistry; D004912:Erythrocytes / Q000737:chemistry / Q000201:enzymology; D005453:Fluorescence; D006801:Humans; D008099:Liver / Q000737:chemistry; D009243:NAD / Q000032:analysis* / Q000097:blood / Q000652:urine; D009249:NADP / Q000032:analysis* / Q000097:blood / Q000652:urine; D009536:Niacinamide / Q000031:analogs & derivatives* / Q000032:analysis / Q000097:blood / Q000652:urine; D051381:Rats
D009243:NAD; D009536:Niacinamide; D009249:NADP; C008472:N-methylnicotinamide