Purification and characterization of streptolysin O from Streptococcus pyogenes.
1. Streptolysin O, an exotoxin produced by group A beta-hemolytic streptococci, has been purified from Streptococcus pyogenes culture supernatants. 2. The isolation and purification procedure involved ammonium sulphate and polyethylene glycol precipitations, and ion-exchange chromatographies on CM-Sepharose and Mono Q. 3. The proposed procedure introduces two ion-exchange chromatography steps making the purification process simpler and, especially, more reproducible than other described protocols. 4. The purified streptolysin O was hemolytically active, had a specific activity of 415,000 HU/mg, an optimum pH of 7.0, a relative molecular mass of 60,100 and an isoelectric pH of 7.5.
Canalias
F
F
Departament de Bioquímica i Biologia Molecular, Universitat Autònoma de Barcelona, Hospital de la Santa Creu i Sant Pau, Spain.
Viver
J
J
Beleta
J
J
Gonzalez-Sastre
F
F
Javier Gella
F
F
D001426:Bacterial Proteins; D004591:Electrophoresis, Polyacrylamide Gel; D004906:Erythrocyte Count; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing; D015203:Reproducibility of Results; D013297:Streptococcus pyogenes / Q000737:chemistry*; D013301:Streptolysins / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000302:isolation & purification*; D013696:Temperature; D013743:Tetanus Antitoxin
D001426:Bacterial Proteins; D013301:Streptolysins; D013743:Tetanus Antitoxin; C025964:streptolysin O