title: [Total chemical synthesis of natural transfer RNA].
abstract: New improvements in the chemical synthesis of oligoribonucleotides are reported and they are applied to the first total chemical synthesis of a natural RNA. This E. coli K12 alanine tRNA contains in its sequence dihydrouridine, ribothymidine and pseudo-uridine. The synthetic tRNA was fully sequenced and showed a 42% aminoacyl acceptance activity. When tRNA was used as a template, reverse transcriptase directed the incorporation of adenine opposite dihydrouridine, ribothymidine and pseudouridine.
authors.affiliation: Laboratoire de Radiobiochimie, S.E.S.A.M./D.R.F.M.C., Grenoble.
authors.forename: D
authors.initials: D
authors.lastname: Gasparutto
authors.forename: T
authors.initials: T
authors.lastname: Livache
authors.forename: A M
authors.initials: AM
authors.lastname: Duplaa
authors.forename: H
authors.initials: H
authors.lastname: Bazin
authors.forename: S
authors.initials: S
authors.lastname: Favario
authors.forename: A
authors.initials: A
authors.lastname: Guy
authors.forename: D
authors.initials: D
authors.lastname: Molko
authors.forename: A
authors.initials: A
authors.lastname: Roget
authors.forename: R
authors.initials: R
authors.lastname: Teoule
mesh_terms: D001483:Base Sequence; D008969:Molecular Sequence Data; D011560:Pseudouridine; D012344:RNA, Transfer, Ala / Q000138:chemical synthesis*; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D014529:Uridine / Q000031:analogs & derivatives
chemical_list: D012344:RNA, Transfer, Ala; D011560:Pseudouridine; C009182:ribothymidine; D012194:RNA-Directed DNA Polymerase; D014529:Uridine