title: Human platelets deficient in dense granules contain normal amounts of pp60c-src.
abstract: We have determined that pp60c-src, a protein tyrosine kinase abundant in normal platelets, is present at comparable levels in platelets that are deficient in dense granules (Hermansky-Pudlak syndrome). Relative quantitation of pp60c-src was performed by immunoblot analysis after protein separation by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Our data suggest that human platelet dense granules, unlike chromaffin cell secretory granules, are not the major intracellular site of localization of pp60c-src.
authors.affiliation: Department of Biochemistry, University of Vermont College of Medicine, Burlington 05405.
authors.forename: A
authors.initials: A
authors.lastname: Sorisky
authors.forename: B
authors.initials: B
authors.lastname: Lages
authors.forename: H J
authors.initials: HJ
authors.lastname: Weiss
authors.forename: S E
authors.initials: SE
authors.lastname: Rittenhouse
mesh_terms: D016115:Albinism, Oculocutaneous / Q000097:blood; D001792:Blood Platelets / Q000378:metabolism*; D006801:Humans; D015151:Immunoblotting; D010981:Platelet Storage Pool Deficiency / Q000097:blood*; D016392:Proto-Oncogene Proteins pp60(c-src) / Q000097:blood*
chemical_list: D016392:Proto-Oncogene Proteins pp60(c-src)