Characterization of an O-antigen bacteriophage from Aeromonas hydrophila.
A unique bacteriophage of Aeromonas hydrophila serotype O:34 was isolated, purified, and characterized. The bacterial surface receptor was shown to be the O-antigen polysaccharide component of lipopolysaccharide specific to serotype O:34, which was chemically characterized. The high molecular weight lipopolysaccharide fraction (a fraction enriched in O antigen) was fully able to inactivate bacteriophage PM1. Phage-resistant mutants of A. hydrophila O:34 were isolated and found to be specifically devoid of lipopolysaccharide O antigen. No other cell-surface molecules were involved in phage binding. The host range of bacteriophage PM1 was found to be very narrow, producing plaques only on A. hydrophila strains from serotype O:34.
Merino
S
S
Departamento de Microbiología, Facultad de Biología, Universidad de Barcelona, Spain.
Camprubí
S
S
Tomás
J M
JM
D000333:Aeromonas / Q000145:classification* / Q000235:genetics / Q000276:immunology; D001435:Bacteriophages / Q000145:classification / Q000302:isolation & purification* / Q000648:ultrastructure; D005947:Glucose / Q000032:analysis; D006595:Hexosamines / Q000032:analysis; D009154:Mutation; D019081:O Antigens; D011135:Polysaccharides, Bacterial / Q000737:chemistry; D011991:Receptors, Virus / Q000276:immunology; D012703:Serotyping
D006595:Hexosamines; D019081:O Antigens; D011135:Polysaccharides, Bacterial; D011991:Receptors, Virus; D005947:Glucose