The human complement C4B/steroid 21-hydroxylase (CYP21) and complement C4A/21-hydroxylase pseudogene (CYP21P) intergenic sequences: comparison and identification of possible regulatory elements.
We determined the 1.8 kb intergenic sequences between the human complement C4B gene and the active steroid 21-hydroxylase gene in two subjects, and between the C4A gene and the steroid 21-hydroxylase pseudogene in one subject. Comparison of these sequences with each other and with published homologues revealed no differences which were unique to either intergenic region. Sequence analysis revealed two copies of an AGGTCA motif in all sequences. This motif is common to steroidogenic enzyme gene promoters and to the response elements for nuclear hormone receptors. Similarities with human enhancers were also found.
Donohoue
P A
PA
Department of Pediatrics, University of Iowa.
Collins
M M
MM
D010582:Bacteriophage lambda / Q000235:genetics; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D015934:Complement C4a / Q000235:genetics*; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D015342:DNA Probes; D006801:Humans; D007438:Introns; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D016133:Polymerase Chain Reaction / Q000379:methods; D011544:Pseudogenes; D012045:Regulatory Sequences, Nucleic Acid; D013255:Steroid 21-Hydroxylase / Q000235:genetics*
D015342:DNA Probes; D009838:Oligodeoxyribonucleotides; D015934:Complement C4a; D004247:DNA; D013255:Steroid 21-Hydroxylase