Monoclonal antibody based capture ELISA/ELIFA for detection of Coxiella burnetii in clinical specimens.
A CAPTURE ELISA/ELIFA system based on monoclonal capture and biotinylated monoclonal detection antibody is described. The assay is fast, highly specific and detects a minimum dose of 2500 Coxiella (C.) burnetii particles. In contrast to the sophisticated and cumbersome isolation procedures, even non-specialized laboratories could use this assay system for investigating clinical samples of different origin for C. burnetii within a short period of time.
Thiele
D
D
Institut für Hygiene und Infektionskrankheiten der Tiere, Justus Liebig Universität, Giessen, F.R.G.
Karo
M
M
Krauss
H
H
D000911:Antibodies, Monoclonal; D001431:Bacteriological Techniques; D016997:Coxiella burnetii / Q000302:isolation & purification*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007124:Immunoenzyme Techniques; D011778:Q Fever / Q000175:diagnosis* / Q000382:microbiology
D000911:Antibodies, Monoclonal