The response of human decidual leukocytes to IL-2.
The phenotype of human decidual leukocytes, composed predominantly of CD3-CD16(-)-CD56bright cells, was examined after culture with IL-2 by immunofluorescence and flow cytometry. After IL-2 stimulation the phenotype became like that found on classical NK cells, with an increased proportion of cells expressing CD16. The IL-2R alpha was absent before and after IL-2 stimulation. However, the intermediate affinity receptor, IL-2R beta, was expressed by CD56bright decidual cells, but this receptor was downregulated after IL-2 stimulation. IL-2-induced proliferation of CD56+ decidual cells could be blocked using TU27, a monoclonal antibody to the IL-2R beta. These findings indicate activation of decidual leukocytes by IL-2 occurs through the IL-2R beta alone.
King
A
A
Department of Pathology, University of Cambridge, United Kingdom.
Wheeler
R
R
Carter
N P
NP
Francis
D P
DP
Loke
Y W
YW
D015703:Antigens, CD / Q000096:biosynthesis; D000943:Antigens, Differentiation / Q000096:biosynthesis*; D000945:Antigens, Differentiation, T-Lymphocyte / Q000096:biosynthesis; D019002:CD56 Antigen; D002455:Cell Division / Q000187:drug effects; D003656:Decidua / Q000187:drug effects / Q000276:immunology*; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology*; D007962:Leukocytes / Q000187:drug effects / Q000276:immunology*; D010641:Phenotype; D011247:Pregnancy / Q000276:immunology*; D011961:Receptors, Fc / Q000096:biosynthesis*; D017452:Receptors, IgG; D015375:Receptors, Interleukin-2 / Q000032:analysis
D015703:Antigens, CD; D000943:Antigens, Differentiation; D000945:Antigens, Differentiation, T-Lymphocyte; D019002:CD56 Antigen; D007376:Interleukin-2; D011961:Receptors, Fc; D017452:Receptors, IgG; D015375:Receptors, Interleukin-2