title: [Selection and physiological study of culture of Bacillus circulans-- producer of butirosin].
abstract: For isolating a highly active variant of the butirosin-producing culture, a strain forming trace amounts of the antibiotic substance was used. Exposure to nitrosomethylbiuret and nitrosoguanidine and the use of selective media containing streptomycin and butirosin resulted in a 30-fold increase in the strain productivity. Thin layer chromatography of the produced antibiotic substance in the solvent system developed by the authors, mass spectrometry and assay of the antimicrobial spectrum in regard to ++gram-positive and ++gram-negative bacteria by using the known aminoglycosidine-inactivating enzymes revealed that the substance was identical to butirosin. Along with the major product, the fermentation broth contained up to 5 per cent of ribostamycin.
authors.forename: Iu D
authors.initials: IuD
authors.lastname: Vikhanskiĭ
authors.forename: V R
authors.initials: VR
authors.lastname: Iarulin
authors.forename: V V
authors.initials: VV
authors.lastname: Esipova
authors.forename: Iu E
authors.initials: IuE
authors.lastname: Bartoshevich
authors.forename: Iu V
authors.initials: IuV
authors.lastname: Zhdanovich
authors.forename: L I
authors.initials: LI
authors.lastname: Nasonova
mesh_terms: D001407:Bacillus / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism; D002076:Butirosin Sulfate / Q000096:biosynthesis* / Q000302:isolation & purification; D002855:Chromatography, Thin Layer / Q000379:methods; D017517:Commonwealth of Independent States; D003470:Culture Media; D066298:In Vitro Techniques; D013058:Mass Spectrometry / Q000379:methods; D013268:Stimulation, Chemical; D013307:Streptomycin / Q000494:pharmacology
chemical_list: D003470:Culture Media; D002076:Butirosin Sulfate; D013307:Streptomycin