title: Characterization of the ilv-2 gene from Neurospora crassa encoding alpha-keto-beta-hydroxylacyl reductoisomerase.
abstract: We have isolated the cDNA and corresponding genomic DNA for the ilv-2 locus of Neurospora crassa. This gene encodes alpha-keto-beta-hydroxylacyl reductoisomerase (Ilv-2), required for the synthesis of isoleucine and valine. The gene contains four introns, maps to the right arm of chromosome V, and encodes a protein of 400 amino acids (aa). Alignment of the aa sequence of Ilv-2 with the two other known eukaryotic sequences encoding this enzyme reveals two conserved regions.
authors.affiliation: Department of Biology, Sinsheimer Laboratories, University of California, Santa Cruz 95064.
authors.forename: H
authors.initials: H
authors.lastname: Sista
authors.forename: B
authors.initials: B
authors.lastname: Bowman
mesh_terms: D015072:2-Acetolactate Mutase / Q000737:chemistry / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D008969:Molecular Sequence Data; D009492:Neurospora crassa / Q000201:enzymology* / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics; D017386:Sequence Homology, Amino Acid
chemical_list: D015072:2-Acetolactate Mutase