Identification of cellular factors that recognize UV-damaged DNA in Drosophila melanogaster.
Using a gel electrophoresis DNA band-shift assay, we have identified 2 DNA-binding protein complexes in wild-type Drosophila embryonic cells which have high affinity for UV-irradiated, double-stranded DNA. Screening of Drosophila mutants deficient in DNA repair led to the identification of 5 mutants which lacked either one of the 2 protein complexes. Four excision repair-deficient mutants (mus-201, phr, mus-308 and mus-205) lacked one protein complex (Factor 2). The other protein complex (Factor 1) was not detectable in the post-replication repair-deficient mutant mus-104. These findings might suggest the possible involvement of these gene products in lesion recognition and repair of UV-induced photoproducts in DNA.
Todo
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Department of Radiation Biology, Faculty of Medicine, Osaka University, Japan.
Ryo
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D000818:Animals; D001483:Base Sequence; D002847:Chromatography, Agarose; D004247:DNA / Q000378:metabolism / Q000528:radiation effects; D004249:DNA Damage; D004260:DNA Repair / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000235:genetics* / Q000528:radiation effects; D008969:Molecular Sequence Data; D009154:Mutation; D014466:Ultraviolet Rays
D004268:DNA-Binding Proteins; D004247:DNA