Quadroma-secreted bi(interferon alpha 2--peroxidase) specific antibody suitable for one-step immunoassay.
A mouse hybridoma (quadroma) was prepared by fusing hybridomas producing monoclonal antibody of G1-isotype to human interferon-alpha 2 with hybridomas producing monoclonal antibody of G2a-isotype against horseradish peroxidase. The established quadroma line secreted immunoglobulins of both G1/G2a-isotypes which manifested parental and bispecific binding characteristics. Culture supernatant containing the bifunctional antibody cross-linking interferon and peroxidase was used for a one-step immunoassay. The developed sandwich ELISA was able to detect the human interferon-alpha 2 at a concentration of 10 units/ml (0, 1 ng/ml) within 2-3 hours.
Kontsekova
E
E
Institute of Virology, Slovak Academy of Sciences, Bratislava.
Kolcunova
A
A
Kontsek
P
P
D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D003432:Cross-Linking Reagents; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006735:Horseradish Peroxidase / Q000276:immunology*; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007370:Interferon Type I / Q000032:analysis / Q000276:immunology*; D051379:Mice; D011994:Recombinant Proteins
D000911:Antibodies, Monoclonal; D003432:Cross-Linking Reagents; D007370:Interferon Type I; D011994:Recombinant Proteins; D006735:Horseradish Peroxidase