[Obtaining and morphological characteristics of primary monolayer cell cultures of human somatotropinomas].
This paper describes the development of method of preparing cell suspension obtained from surgical material of patients with pituitary adenomas and acromegaly as well as the procedure of subsequent long-term cultivation in monolayers of the cells isolated. As judged by visual inspection and measurement of growth hormone and prolactin secretion, tumor pituitary cells kept viability and functional activity for at least 6 days of growing in vitro. Immunocytochemical visualization of somato- and lactotrophs of the same histological preparations permitted us to show that vast majority of cultured cells is represented by somatotrophs; however, a small portion of cell population is represented by lactotrophs and lactosomatotrophs. The peculiarities of cytoarchitectonics in two types of cell cultures of human somatotropinomas were studied.
Komolov
I S
IS
Bulatov
A A
AA
Trunin
Iu K
IuK
Serpukhovitin
S Iu
SIu
Marova
E I
EI
Dedov
I I
II
D000172:Acromegaly; D000236:Adenoma / Q000378:metabolism / Q000473:pathology*; D000328:Adult; D013006:Growth Hormone / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D010911:Pituitary Neoplasms / Q000378:metabolism / Q000473:pathology*; D011388:Prolactin / Q000378:metabolism; D013997:Time Factors; D014407:Tumor Cells, Cultured / Q000378:metabolism
D011388:Prolactin; D013006:Growth Hormone