Effects of insulin-like growth factors and growth hormone on the in vitro proliferation of T lymphocytes.
The insulin-like growth factors I and II (IGF-I and IGF-II) promote proliferation and differentiation of many cell types. We report that recombinant IGF-I and IGF-II augment both the lectin- and anti-CD3-induced proliferation of human peripheral blood mononuclear cells (PBMC) at concentrations proportional to their binding affinities. IGF-I and IGF-II also augmented the lectin-induced proliferation of purified T lymphocytes. Effects of IGF-I were found in cultures of T cells vigorously depleted for monocytes and supplemented with saturating concentrations of interleukin-1. The latter results indicate that the effect of IGF-I on the proliferation of T lymphocytes can occur independent of monocytes or monocyte-derived factors.
Kooijman
R
R
Department of Immunology, University Hospital for Children, Utrecht, The Netherlands.
Willems
M
M
Rijkers
G T
GT
Brinkman
A
A
van Buul-Offers
S C
SC
Heijnen
C J
CJ
Zegers
B J
BJ
D002352:Carrier Proteins / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D013006:Growth Hormone / Q000494:pharmacology*; D006801:Humans; D018969:Insulin-Like Growth Factor Binding Proteins; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology*; D007335:Insulin-Like Growth Factor II / Q000494:pharmacology*; D009000:Monocytes / Q000166:cytology; D011994:Recombinant Proteins; D013002:Somatomedins / Q000494:pharmacology; D013601:T-Lymphocytes / Q000166:cytology*
D002352:Carrier Proteins; D018969:Insulin-Like Growth Factor Binding Proteins; D011994:Recombinant Proteins; D013002:Somatomedins; D007334:Insulin-Like Growth Factor I; D007335:Insulin-Like Growth Factor II; D013006:Growth Hormone