title: Differential adhesion of granulocytes to five distinct phenotypes of cultured microvascular endothelial cells.
abstract: Adhesion of isolated human polymorphonuclear granulocytes (PMNs) to five different phenotypes of cultured microvascular endothelial cells derived from bovine corpora lutea was investigated by measuring the myeloperoxidase content of cell lysates. Untreated and interleukin 1 (IL-1) -pretreated confluent monolayers were overlaid with unstimulated and phorbol ester (PMA)-stimulated PMNs in the absence and presence of the monoclonal antibody IB4 recognizing and functionally blocking beta 2 (CD18) of the leukocyte integrins. Unstimulated PMN adhesion was highest on type 4, followed by type 3 and 5 endothelial cells. This adhesion was not inhibited by treatment with IB4. IL-1 pretreatment of endothelial cells resulted in a significant increase of PMN adhesion on types 1, 2, and 4, most of which was also beta 2 integrin-independent. PMA-stimulation of PMNs increased adhesion to maximal values on cell types 1 and 5, which was largely blocked by IB4. Type 2 endothelial cells supported significantly less PMA-stimulated PMN adhesion than all other types. In the presence of IB4, adhesion of PMNs to untreated and IL-1-pretreated type 3 and 4 endothelial cells was significantly reduced by PMA. This reduction of beta 2 integrin-independent adhesion by PMA stimulation is compatible with possible shedding of the lectin-like leukocyte adhesion molecule, L-selectin, from PMNs. Differential PMN adhesion may reflect distinctive expression of endothelial adhesion molecules in different phenotypes of microvascular endothelial cells. Endothelial specialization within the microcirculation may have important functional consequences for the inflammatory response in vivo.
authors.affiliation: Department of Physiology, Freie Universität Berlin, Germany.
authors.forename: K
authors.initials: K
authors.lastname: Ley
authors.forename: P
authors.initials: P
authors.lastname: Gaehtgens
authors.forename: K
authors.initials: K
authors.lastname: Spanel-Borowski
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000502:physiology; D018821:CD18 Antigens; D002417:Cattle; D002448:Cell Adhesion / Q000187:drug effects; D015815:Cell Adhesion Molecules / Q000502:physiology; D019040:E-Selectin; D004730:Endothelium, Vascular / Q000502:physiology*; D006098:Granulocytes / Q000502:physiology*; D006801:Humans; D066298:In Vitro Techniques; D007375:Interleukin-1 / Q000494:pharmacology; D008855:Microscopy, Electron, Scanning; D009195:Peroxidase / Q000032:analysis; D010641:Phenotype; D013755:Tetradecanoylphorbol Acetate
chemical_list: D015703:Antigens, CD; D018821:CD18 Antigens; D015815:Cell Adhesion Molecules; D019040:E-Selectin; D007375:Interleukin-1; D009195:Peroxidase; D013755:Tetradecanoylphorbol Acetate