title: A ten-minute protocol for transforming Saccharomyces cerevisiae by electroporation.
abstract: We present a simplified and rapid method for the transformation of yeast cells by electroporation. Stationary cells, scraped off the agar of Petri dish cultures stored in the refrigerator for up to 6 weeks, are suspended in sorbitol buffer, spun down by gentle centrifugation, transferred into the electroporation cuvette, and immediately subjected to transformation via electroporation. Transformation efficiency of this 10-min method, which does not require the preparation of cell cultures, is about 10% of the hitherto best performing transformation procedure using cells of defined growth phase.
authors.affiliation: Institut für Mikrobiologie der J. W. Goethe-Universität, Frankfurt/Main, Federal Republic of Germany.
authors.forename: M
authors.initials: M
authors.lastname: Grey
authors.forename: M
authors.initials: M
authors.lastname: Brendel
mesh_terms: D004271:DNA, Fungal; D004560:Electricity; D010957:Plasmids; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D014162:Transfection / Q000379:methods*; D014170:Transformation, Genetic
chemical_list: D004271:DNA, Fungal