title: Evaluating retinal circulation using video fluorescein angiography in control and diabetic rats.
abstract: Video fluorescein angiography has been used to evaluate retinal circulatory parameters in diabetic and non-diabetic Sprague-Dawley rats. Video fluorescein angiograms were recorded from the retina using a modified retinal fundus camera following a 5 ul bolus injection of sodium fluorescein dye into the jugular vein. Retinal circulatory parameters were measured using computer assisted image analysis. These analyses were performed on 25 diabetic rats with 1 week duration of diabetes and 26 matched, non-diabetic, rats. There was a significant (p = .0001) increase in retinal Mean Circulation Time (MCT) in the diabetic group (1.83 +/- 0.40 s) compared to the control group (1.09 +/- 0.27 s). There were no significant differences in arterial or venous diameters comparing diabetic and control groups. In a separate paired experiment, measurements were made from the same animals both before and after one week duration of diabetes. A paired t-test analysis demonstrated significantly increased MCT times in the 6 diabetic animals (p = .001) while there was no significant differences detected in the 4 corresponding control animals. These results indicate that significant increases in retinal circulation times can be measured as early as 1 week after streptozotocin induced diabetes in this animal model.
authors.affiliation: Beetham Eye Institute, Joslin Diabetes Center, Boston, MA.
authors.forename: S E
authors.initials: SE
authors.lastname: Bursell
authors.forename: A C
authors.initials: AC
authors.lastname: Clermont
authors.forename: T
authors.initials: T
authors.lastname: Shiba
authors.forename: G L
authors.initials: GL
authors.lastname: King
mesh_terms: D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000503:physiopathology; D003930:Diabetic Retinopathy / Q000503:physiopathology*; D005451:Fluorescein Angiography / Q000379:methods*; D007091:Image Processing, Computer-Assisted; D008137:Longitudinal Studies; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012039:Regional Blood Flow; D015203:Reproducibility of Results; D012171:Retinal Vessels / Q000503:physiopathology*; D013311:Streptozocin; D014741:Video Recording
chemical_list: D013311:Streptozocin