Specific activation of platelets from patients allergic to Dermatophagoides pteronyssinus by synthetic peptides derived from the allergen Der p I.
Previous studies have shown that Fc epsilon RII-bearing platelets from patients with allergic disorders could be stimulated either by anti-human IgE antibodies or the relevant allergens to generate cytotoxic mediators. In this report, the reactivity of platelets from Dermatophagoides pteronyssinus (D. pt)-sensitive patients to three Der p I-derived synthetic peptides (52-71, 117-133 and 188-199) was investigated. The platelet stimulation observed in D. pt-sensitive patients was demonstrated to be allergen-specific and to be mediated by IgE. These Der p I-derived peptides failed to stimulate platelets from healthy donors or platelets from non-D. pt-allergic patients. D. pt-sensitive patients responded more frequently to the peptides 52-71 and 117-133 than to the peptide 188-199. Thus, in this model, synthetic peptides selected for their assumed accessibility on the native antigen could be associated to an IgE-dependent biological activity.
Cardot
E
E
Inserum CJF 90-06 URA CNRS 1309, Institut Pasteur, Lille, France.
Pestel
J
J
Callebaut
I
I
Lassalle
P
P
Tsicopoulos
A
A
Gras-Masse
H
H
Capron
A
A
Joseph
M
M
D000328:Adult; D000485:Allergens / Q000737:chemistry / Q000276:immunology*; D000595:Amino Acid Sequence; D000818:Animals; D039741:Antigens, Dermatophagoides; D000944:Antigens, Differentiation, B-Lymphocyte / Q000502:physiology*; D006801:Humans; D006967:Hypersensitivity / Q000276:immunology*; D007073:Immunoglobulin E / Q000276:immunology; D066298:In Vitro Techniques; D008875:Middle Aged; D008925:Mites / Q000276:immunology*; D008969:Molecular Sequence Data; D010455:Peptides / Q000276:immunology*; D015539:Platelet Activation; D011961:Receptors, Fc / Q000502:physiology*; D017455:Receptors, IgE
D000485:Allergens; D039741:Antigens, Dermatophagoides; D000944:Antigens, Differentiation, B-Lymphocyte; D010455:Peptides; D011961:Receptors, Fc; D017455:Receptors, IgE; D007073:Immunoglobulin E