Cloning and characterisation of an oleosin gene from Brassica napus.
The sequence of an oleosin gene from Brassica napus has been determined. This gene contains a single intron of 437 bp and encodes a polypeptide of 195 amino acids. The oleosin gene product has an estimated molecular mass of 21.5 kDa and consists of a highly hydrophobic central domain flanked by relatively polar N- and C-terminal domains. The central domain is highly conserved between all oleosins sequenced to date and contains a run of periodically spaced leucine residues similar to that of a leucine-zipper motif. The gene has been shown to be expressed specifically in the embryo, maximally between 9 and 11 weeks after flowering, i.e. during the seed desiccation stage. Two transcriptional start sites have been mapped to -70 and -21 of the ATG and a putative ABA-responsive element and three repeated motifs have been identified in the promoter. These short promoter sequences could correspond to regulatory elements responsible for embryo-specific gene expression. Up to six genes exist in the oleosin gene family.
Keddie
J S
JS
Department of Brassica and Oilseeds Research, Cambridge Laboratory, John Innes Centre for Plant Science Research, Norwich, UK.
Hübner
G
G
Slocombe
S P
SP
Jarvis
R P
RP
Cummins
I
I
Edwards
E W
EW
Shaw
C H
CH
Murphy
D J
DJ
D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D001937:Brassica / Q000235:genetics*; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D008074:Lipoproteins / Q000235:genetics*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009838:Oligodeoxyribonucleotides; D010940:Plant Proteins / Q000235:genetics*; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
D008074:Lipoproteins; D009838:Oligodeoxyribonucleotides; D010940:Plant Proteins; C079356:oleosin protein, Brassica napus; D012313:RNA; D004247:DNA