Molecular cloning and chromosomal mapping of CCND genes encoding human D-type cyclins.
A human D-type cyclin gene (CCND1/cyclin D1/PRAD1) was previously isolated by virtue of its ability to complement a triple G1 cyclin (Cln) deficiency of Saccharomyces cerevisiae and was also identified as a candidate BCL1 oncogene. We now report the molecular cloning of two additional human D-type cyclin genes, CCND2 (cyclin D2) and CCND3 (cyclin D3). All three human D-type cyclin genes encode small (33-34 kDa) proteins that share an average of 57% identity over the entire coding region and 78% in the cyclin box. The D-type cyclins are most closely related to cyclin A (39% identity) and cyclin E (36%), followed by cyclin B (29%) and cyclin C (21%). Isolation and characterization of genomic clones revealed two pseudogenes corresponding to CCND2 and CCND3, respectively. All three cyclin D genes are interrupted by an intron at the same position. CCND2 has been mapped to chromosome 12p13, and CCND3 has been mapped to chromosome 6p21.
Xiong
Y
Y
Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, New York 11724.
Menninger
J
J
Beach
D
D
Ward
D C
DC
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D016213:Cyclins / Q000235:genetics*; D004247:DNA / Q000235:genetics; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D011544:Pseudogenes; D012689:Sequence Homology, Nucleic Acid
D016213:Cyclins; D004247:DNA