The development of enzyme-linked immunosorbent assays (ELISA) for the catecholamines adrenalin and noradrenalin.
Monoclonal antibodies have been raised against adrenalin and noradrenalin and used as the basis of enzyme-linked immunosorbent assays (ELISA) to detect and estimate the concentrations of these catecholamines. Inhibition assays are described, with sensitive quantification in the range from 1 mg/ml to 100 pg/ml. Cross-reactivity assays reveal that neither assay is subject to interference by catecholamine metabolites at concentrations less than 100 ng/ml and 1 micrograms/ml respectively. Isolation and quantification of both catecholamines from clinical samples is discussed and the potential application of these ELISAs in a clinical setting is proposed.
Murphy
J F
JF
Faculty of Science and Innovation, NEWI, Deeside, Clwyd, UK.
Davies
D H
DH
Smith
C J
CJ
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology*; D002395:Catecholamines / Q000276:immunology; D003429:Cross Reactions; D004306:Dose-Response Relationship, Immunologic; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D004837:Epinephrine / Q000032:analysis* / Q000276:immunology / Q000302:isolation & purification; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D051379:Mice; D008807:Mice, Inbred BALB C; D009638:Norepinephrine / Q000032:analysis* / Q000276:immunology / Q000302:isolation & purification; D012680:Sensitivity and Specificity
D000911:Antibodies, Monoclonal; D002395:Catecholamines; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D009638:Norepinephrine; D004837:Epinephrine