Cloning and sequencing of the 23 kDa mouse photoreceptor cell-specific protein.
The 23 kDa protein was localized by immunocytochemistry to photoreceptor cells of the mouse retina, and bovine and mouse cDNA clones were isolated and sequenced. The deduced amino acid sequences showed that the mouse 23 kDa protein is 91% identical to the bovine protein, and is the same as S-modulin, the CAR (cancer-associated retinopathy) protein and recoverin, the Ca(2+)-dependent activator of photoreceptor guanylate cyclase. The amino acid sequence reveals two Ca2+ binding sites, no internal repeats, 59% homology to the chicken visinin protein and 40% homology to calmodulin while Northern analysis demonstrated a single 1.0 kb mRNA species in bovine and mouse retina.
McGinnis
J F
JF
Department of Anatomy and Cell Biology, University of California, Los Angeles 90024.
Stepanik
P L
PL
Baehr
W
W
Subbaraya
I
I
Lerious
V
V
D000595:Amino Acid Sequence; D000818:Animals; D000951:Antigens, Neoplasm / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D001665:Binding Sites; D002118:Calcium / Q000378:metabolism; D002135:Calcium-Binding Proteins / Q000737:chemistry / Q000235:genetics*; D002147:Calmodulin / Q000737:chemistry; D002417:Cattle; D003001:Cloning, Molecular; D004247:DNA / Q000737:chemistry / Q000302:isolation & purification; D005136:Eye Proteins; D051597:Hippocalcin; D007150:Immunohistochemistry; D008074:Lipoproteins; D051379:Mice; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009419:Nerve Tissue Proteins / Q000737:chemistry; D010786:Photoreceptor Cells / Q000737:chemistry*; D016133:Polymerase Chain Reaction; D051603:Recoverin; D012160:Retina / Q000737:chemistry; D012689:Sequence Homology, Nucleic Acid
D000951:Antigens, Neoplasm; D002135:Calcium-Binding Proteins; D002147:Calmodulin; D005136:Eye Proteins; D008074:Lipoproteins; D009419:Nerve Tissue Proteins; C496732:Rcvrn protein, mouse; C045140:visinin; D051603:Recoverin; D051597:Hippocalcin; D004247:DNA; D002118:Calcium