Artifactual detection of ADP-dependent sucrose synthase in crude plant extracts.
Results presented in a previous report from this laboratory indicated the presence, in crude extracts from sycamore (Acer pseudoplatanus) and spinach (Spinacea oleracea), of a sucrose synthase (EC 2.4.1.13) showing high affinity for ADP as the glucose acceptor in the sucrose-cleaving reaction. In the present paper we report that the modified enzymatic method previously used to measure sucrose synthase activities leads to the detection of artifactual ADP-dependent sucrose synthase, which in fact arises from the combined action of invertase (EC 3.2.1.26) and nucleoside diphosphate kinase (EC 2.7.4.6) activities. We also present data on the partial purification of nucleoside diphosphate kinase from sycamore cells.
Perata
P
P
Research Institute for Biochemical Regulation, School of Agricultural Sciences, Nagoya University, Japan.
Pozueta-Romero
J
J
Yamaguchi
J
J
Akazawa
T
T
D000244:Adenosine Diphosphate / Q000378:metabolism*; D002855:Chromatography, Thin Layer; D004591:Electrophoresis, Polyacrylamide Gel; D005964:Glucosyltransferases / Q000378:metabolism*; D010936:Plant Extracts / Q000737:chemistry*
D010936:Plant Extracts; D000244:Adenosine Diphosphate; D005964:Glucosyltransferases; C022699:sucrose synthase