Effects of liposome encapsulated hemoglobin on the reticuloendothelial system.
The effects of different doses (4, 10, and 25%) of liposome encapsulated hemoglobin (LEH) were measured on the Reticuloendothelial System (RES) and Kupffer cells (KC) by i) colloidal carbon clearance in the rat in vivo and in the isolated perfused liver, ii) magnetometry, and iii) histological analysis. At the highest dose, in vivo carbon clearance rates (k) were half the rate as in controls at 2 and 12 hours post-treatment. By 24 hours post-treatment clearance rates were at control levels. Empty liposomes (LIP) caused a 2-fold decrease in k at 2 hours only. With both LEH and LIP, the effects were less severe when rats were given the lower doses. Magnetometric studies showed a decrease in KC phagosomal motion in the LEH-treated (25%) rats at 2 and 24 hours that returned to control levels at 2 weeks. Perfused livers from rats treated with a low dose of LEH cleared carbon at the same rate as LIP and Krebs Ringer Bicarbonate (KRB) controls. Histological examination showed minimal tissue damage in all test groups. Thus, LEH and LIP have some short-term deleterious effects on KC and the RES probably due to RES blockade rather than cellular damage.
Beach
M C
MC
Barnard College, Columbia University, New York, New York 10027.
Morley
J
J
Spiryda
L
L
Weinstock
S B
SB
D000818:Animals; D001802:Blood Substitutes / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000633:toxicity*; D006454:Hemoglobins / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000633:toxicity*; D066298:In Vitro Techniques; D007728:Kupffer Cells / Q000187:drug effects / Q000378:metabolism; D008081:Liposomes; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008297:Male; D012157:Mononuclear Phagocyte System / Q000187:drug effects* / Q000378:metabolism; D010587:Phagocytosis / Q000187:drug effects; D051381:Rats
D001802:Blood Substitutes; D006454:Hemoglobins; D008081:Liposomes