Hamster female protein binding to chromatin, histones and DNA.
Hamster female protein (FP) is a member of the family of proteins known as pentraxins which share amino acid sequence homology, cyclic pentameric structure and calcium-dependent binding to ligands. Other members of this family include C-reactive protein (CRP) and serum amyloid P component (SAP), and most species synthesize both CRP and SAP. FP is unusual in that it is apparently the only pentraxin produced in hamsters, it is under hormonal control and it shares binding characteristics with both CRP and SAP. CRP has been defined and isolated by its calcium-dependent binding to pneumococcal C-polysaccharide via phosphocholine (PC) residues. SAP has been isolated by calcium-dependent binding to agarose. FP binds to both PC and agarose. Recently, both SAP and CRP have been found to bind to chromatin in a calcium-dependent manner and involvement of these proteins in the clearance of nuclear material has been proposed. In this paper we test whether FP shares the ability to bind to chromatin and histones, and compare its relative avidities for these ligands. Similar to CRP, FP bound to histones H1 and H2A, and chromatin. FP shared with SAP the ability to bind to DNA. However, FP binding was inhibited by PC for all ligands, whereas SAP binding was not. FP and SAP also failed to compete with each other for binding to DNA. By cross-inhibition FP bound much less well to PC than CRP, but was a very effective inhibitor of CRP binding to H2A. These studies demonstrate that chromatin and histone binding are conserved among these pentraxins. The role of the proposed PC binding site in these binding reactions is discussed.
Saunero-Nava
L
L
VA Medical Center, Albuquerque, NM 87108.
Coe
J E
JE
Mold
C
C
Du Clos
T W
TW
D000510:Alpha-Globulins / Q000737:chemistry / Q000378:metabolism*; D000595:Amino Acid Sequence; D002097:C-Reactive Protein / Q000378:metabolism; D002843:Chromatin / Q000378:metabolism*; D004247:DNA / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism; D006657:Histones / Q000378:metabolism*; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D010767:Phosphorylcholine / Q000378:metabolism; D011485:Protein Binding
D000510:Alpha-Globulins; D002843:Chromatin; D004268:DNA-Binding Proteins; D006657:Histones; D046911:Macromolecular Substances; C030974:female protein, hamster; D010767:Phosphorylcholine; D002097:C-Reactive Protein; D004247:DNA