Regulatory role of GM3 ganglioside in integrin function, as evidenced by its effect on function of alpha 5 beta 1-liposomes: a preliminary note.
Mouse mammary carcinoma mutant cell line FUA169, characterized by high GM3 ganglioside content, was established from parent cell line FM3A/F28-7, which has high LacCer content but no GM3. Although both cell lines showed the same quantity and quality of integrin receptors, FUA169 showed much stronger adhesion to fibronectin (FN)-coated plates than did F28-7. Liposomes containing phosphatidylcholine, cholesterol, alpha 5 beta 1, and a moderate amount of GM3 showed greatly enhanced adhesion to FN-coated plates, but adhesion of similar liposomes containing a large amount of GM3, or no GM3, was much lower. Our results suggest that GM3 regulates integrin receptor function essential for cell adhesion to FN.
Zheng
M
M
Biomembrane Institute, Seattle, WA 98119.
Tsuruoka
T
T
Tsuji
T
T
Hakomori
S
S
D000818:Animals; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D002784:Cholesterol; D005353:Fibronectins / Q000502:physiology*; D005679:G(M3) Ganglioside / Q000494:pharmacology* / Q000502:physiology; D016023:Integrins / Q000378:metabolism*; D008081:Liposomes; D008325:Mammary Neoplasms, Experimental; D051379:Mice; D008969:Molecular Sequence Data; D010713:Phosphatidylcholines; D017469:Receptors, Fibronectin; D011971:Receptors, Immunologic / Q000378:metabolism
D005353:Fibronectins; D005679:G(M3) Ganglioside; D016023:Integrins; D008081:Liposomes; D010713:Phosphatidylcholines; D017469:Receptors, Fibronectin; D011971:Receptors, Immunologic; D002784:Cholesterol