Semi-dry electroblotting of DNA and RNA from agarose and polyacrylamide gels.
Semi-dry electroblotting of nucleic acids was originally of interest for its speed and convenience, but it had significant limitations in reliability of transfer. This work describes optimalization of the buffer systems and general conditions for use that enable the semi-dry method of nucleic acids transfer to yield quantitative, fast and reliable results.
Trnovsky
J
J
D004247:DNA / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D005456:Fluorescent Dyes; D012313:RNA / Q000302:isolation & purification*
D005456:Fluorescent Dyes; D012313:RNA; D004247:DNA