Characterization of a feline T-cell-specific monoclonal antibody reactive with a CD5-like molecule.
The 43 monoclonal antibody raised against feline T cells was found to react with a single-chain glycoprotein of Mr 72,000 that is present on most thymocytes, 60% of lymph node cells, 20% of splenocytes, and 45% of blood mononuclear cells. All CD4+ and CD8+ T cells were found to express the 43-reactive determinant, as did a small subpopulation of CD4-/CD8-/IgM- lymphocytes in the periphery. The 43-reactive determinant was not detected on B cells, macrophages, or other types of blood cells. The 43 antigen was phosphorylated in resting and activated T cells. Its expression was upregulated by stimulation with phorbol myristate acetate and with phytohemagglutinin. When added to concanavalin A-stimulated T-cell cultures in low concentrations, the 43 antibody was found to augment mitogenesis. The data indicate that this antibody may identify a CD5 homologue on feline T cells.
Ackley
C D
CD
Department of Pediatrics, Medicine and Microbiology, School of Medicine, University of Alabama, Birmingham.
Cooper
M D
MD
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D015703:Antigens, CD / Q000276:immunology*; D015704:CD4 Antigens / Q000276:immunology; D018956:CD5 Antigens; D016827:CD8 Antigens / Q000276:immunology; D002415:Cats / Q000276:immunology*; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000276:immunology; D005455:Fluorescent Antibody Technique; D006825:Hybridomas; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008221:Lymphoid Tissue / Q000276:immunology*; D051379:Mice; D010766:Phosphorylation; D013601:T-Lymphocytes / Q000276:immunology*; D013950:Thymus Gland / Q000276:immunology; D014018:Tissue Distribution
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D015704:CD4 Antigens; D018956:CD5 Antigens; D016827:CD8 Antigens; D000939:Epitopes