title: A bifunctional enzyme (delta 1-pyrroline-5-carboxylate synthetase) catalyzes the first two steps in proline biosynthesis in plants.
abstract: Many plants synthesize and accumulate proline in response to osmotic stress. Despite the importance of this pathway, however, the exact metabolic route and enzymes involved in the synthesis of proline in plants have not been unequivocally identified. We report here the isolation of a mothbean (Vigna aconitifolia) cDNA clone encoding a bifunctional enzyme, delta 1-pyrroline-5-carboxylate synthetase (P5CS), with both gamma-glutamyl kinase and glutamic-gamma-semialdehyde dehydrogenase activities that catalyzes the first two steps in proline biosynthesis. The two enzymatic domains of P5CS correspond to the ProB and ProA proteins of Escherichia coli and contain a leucine zipper in each domain, which may facilitate inter- or intramolecular interaction of this protein. The Vigna P5CS enzyme activity is feedback regulated by proline but is less sensitive to end-product inhibition than is the E. coli gamma-glutamyl kinase. The P5CS gene is expressed at high levels in Vigna leaves and is inducible in roots subjected to salt stress, suggesting that P5CS plays a key role in proline biosynthesis, leading to osmoregulation in plants.
authors.affiliation: Department of Molecular Genetics, Ohio State University, Columbus 43210-1002.
authors.forename: C A
authors.initials: CA
authors.lastname: Hu
authors.forename: A J
authors.initials: AJ
authors.lastname: Delauney
authors.forename: D P
authors.initials: DP
authors.lastname: Verma
mesh_terms: D050842:1-Pyrroline-5-Carboxylate Dehydrogenase; D000445:Aldehyde Oxidoreductases / Q000235:genetics* / Q000378:metabolism*; D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D005246:Feedback; D050698:Glutamate-5-Semialdehyde Dehydrogenase; D007700:Kinetics; D008969:Molecular Sequence Data; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000235:genetics* / Q000378:metabolism*; D010770:Phosphotransferases / Q000235:genetics* / Q000378:metabolism*; D017851:Phosphotransferases (Carboxyl Group Acceptor); D010944:Plants / Q000201:enzymology* / Q000235:genetics; D011061:Poly A / Q000235:genetics / Q000302:isolation & purification; D011392:Proline / Q000096:biosynthesis* / Q000494:pharmacology; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012333:RNA, Messenger; D017386:Sequence Homology, Amino Acid
chemical_list: D012333:RNA, Messenger; D011061:Poly A; D012313:RNA; D004247:DNA; D011392:Proline; D000445:Aldehyde Oxidoreductases; D050698:Glutamate-5-Semialdehyde Dehydrogenase; D050842:1-Pyrroline-5-Carboxylate Dehydrogenase; D000587:Oxidoreductases Acting on CH-NH Group Donors; D010770:Phosphotransferases; D017851:Phosphotransferases (Carboxyl Group Acceptor); C020650:glutamate 5-kinase