Flow-injection analysis for malondialdehyde in plasma with the thiobarbituric acid reaction.
A simple, precise, and rapid method to measure plasma malondialdehyde (MDA) was developed by use of solvent extraction--flow-injection analysis. The reagent solution, containing thiobarbituric acid (TBA), 5 g/L in 100 mL/L phosphoric acid, and extraction solvent (methylisobutyl ketone, MIBK) were propelled with a double-plunger micropump at a flow rate of 0.3 mL/min, and 20 microL of sample was introduced into the reagent stream. After TBA-MDA reactant was extracted into MIBK, the organic phase was continuously separated by a successive phase-separation system equipped with two phase separators, and the absorbance of the TBA-MDA reactant was measured at 532 nm. This approach resulted in excellent sensitivity, a CV of < 1.5%, a good correlation with the conventional manual method, and a sampling frequency of 7 samples/h, suggesting that this semiautomated method is suitable for measuring plasma MDA.
Ikatsu
H
H
Department of Hygiene, Shinshu University School of Medicine, Matsumoto-shi, Japan.
Nakajima
T
T
Murayama
N
N
Korenaga
T
T
D000818:Animals; D001322:Autoanalysis / Q000379:methods*; D002251:Carbon Tetrachloride / Q000494:pharmacology; D017022:Flow Injection Analysis / Q000295:instrumentation / Q000379:methods* / Q000592:standards; D008054:Lipid Peroxides / Q000097:blood*; D008297:Male; D008315:Malondialdehyde / Q000097:blood*; D008742:Methyl n-Butyl Ketone; D051381:Rats; D017208:Rats, Wistar; D012997:Solvents; D013696:Temperature; D013858:Thiobarbiturates; D013997:Time Factors
D008054:Lipid Peroxides; D012997:Solvents; D013858:Thiobarbiturates; D008315:Malondialdehyde; D008742:Methyl n-Butyl Ketone; D002251:Carbon Tetrachloride; C029684:thiobarbituric acid; C005458:methyl isobutyl ketone