title: Biocompatibility of extracorporeal circulation with autooxygenation.
abstract: Platelet damage, complement activation and neutropenia during cardiopulmonary bypass are the result of blood contact with artificial surfaces, mainly in the oxygenator. To evaluate biocompatibility of this kind of bypass we compared two techniques of extracorporeal circulation in 40 patients undergoing elective coronary bypass operations. In 20, a standard technique with a bubble oxygenator was used (group 1), and in the remaining 20 patients with autooxygenation, the patients' own lungs were included in the perfusion circuit (group 2). Several blood samples were taken before, during and after perfusion to estimate the corrected platelet numbers and pulmonary leucocyte sequestration in all patients, and additionally in 6 patients from each group, complement C3a and C5a anaphylatoxins were measured (radioimmunoassay). At the end of cardiopulmonary bypass, the decline of platelet number corrected to haematocrit platelet number in group 1 was significantly higher than in group 2 (P less than 0.01). There was a significant increase in circulating white blood cells when compared to pre-bypass time in both groups (P less than 0.05). However, comparison of differences between leucocyte counts in the blood of the patients' right and left atria showed enhanced leucocyte sequestration in group 1, 1.46 +/- 0.5 x 10(3)/mm3 vs only 0.34 +/- 0.2 x 10(3)/mm3 in group 2. The C3a rose progressively during extracorporeal circulation: in group 1 from 268 +/- 46 ng/l to 521 +/- 65 ng/l, and in group 2 from 244 +/- 46 ng/l to 418 +/- 34 ng/l (P less than 0.05). No characteristic changes in C5a activation were observed in either group.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: 1st Clinic of Cardiac Surgery, Silesian Medical Academy, Silesian Heart Center, Katowice, Poland.
authors.forename: A
authors.initials: A
authors.lastname: Bochenek
authors.forename: Z
authors.initials: Z
authors.lastname: Religa
authors.forename: F
authors.initials: F
authors.lastname: Kokot
authors.forename: A M
authors.initials: AM
authors.lastname: Wnuk-Wojnar
authors.forename: J
authors.initials: J
authors.lastname: Wojnar
authors.forename: R
authors.initials: R
authors.lastname: Wnuk
authors.forename: G
authors.initials: G
authors.lastname: Gallert
authors.forename: J
authors.initials: J
authors.lastname: Skiba
mesh_terms: D016063:Blood Loss, Surgical; D001792:Blood Platelets / Q000502:physiology; D002315:Cardiopulmonary Bypass / Q000295:instrumentation* / Q000379:methods; D003167:Complement Activation; D015926:Complement C3a / Q000032:analysis; D015936:Complement C5a / Q000032:analysis; D006355:Heart-Lung Machine; D006454:Hemoglobins / Q000032:analysis; D006801:Humans; D007958:Leukocyte Count; D008875:Middle Aged
chemical_list: D006454:Hemoglobins; D015926:Complement C3a; D015936:Complement C5a