Total chromium in the human lens. Determination with Zeeman electrothermal atomic absorption spectrometry following mineralization in a mini-autoclave.
A simple, quick procedure has been developed for preparation of human lenses for total chromium (Cr) analysis by electrothermal atomic absorption spectrometry (EAAS). This procedure involves wet ashing in a mini-autoclave with HNO3 and H2SO4. Recovery was 101.6%. This procedure is simple to carry out, does not generate corrosive fumes, and minimizes contaminations. Measurements obtained by this method give values similar to those found previously by more cumbersome methods. It can be conveniently used to prepare biological samples for ultratrace analysis. The mean Cr concentration in human lenses varied between 0.345 +/- 0.147 micrograms/g dry wt in the normal population and 0.205 +/- 0.160 micrograms/g dry wt in cataractous lenses.
Pineau
A
A
Laboratoire de Toxicologie, Faculté de Pharmacie, Nantes, France.
Guillard
O
O
Chauvelon
F
F
Risse
J F
JF
D002386:Cataract / Q000378:metabolism; D002623:Chemistry Techniques, Analytical; D002857:Chromium / Q000032:analysis*; D006801:Humans; D007908:Lens, Crystalline / Q000737:chemistry*; D013054:Spectrophotometry, Atomic / Q000379:methods
D002857:Chromium