title: A comparison of two autoradiographic methods for detecting radiolabeled nucleic acids in embryos.
abstract: Two methods for preparing embryos for autoradiographic study of newly synthesized nucleic acids are described and compared. The first method consists of rapidly fixing radiolabeled embryos with acetic acid:methanol, spreading them on glass slides and exposing them for 8 days with a photographic emulsion. The second method consists of fixing, embedding in resin, and sectioning the embryos before their exposure with the emulsion for 3 weeks. Both techniques have many applications in studies of early embryonic activity, but the spread technique is very sensitive, simpler, and faster.
authors.affiliation: Department of Biomedical Sciences, University of Guelph, Ontario, Canada.
authors.forename: L
authors.initials: L
authors.lastname: Plante
authors.forename: J W
authors.initials: JW
authors.lastname: Pollard
authors.forename: W A
authors.initials: WA
authors.lastname: King
mesh_terms: D000818:Animals; D001345:Autoradiography / Q000379:methods*; D001755:Blastocyst / Q000737:chemistry* / Q000648:ultrastructure; D002417:Cattle / Q000196:embryology; D004247:DNA / Q000032:analysis* / Q000096:biosynthesis; D012313:RNA / Q000032:analysis* / Q000096:biosynthesis; D013048:Specimen Handling
chemical_list: D012313:RNA; D004247:DNA