title: Cell lines produce factors that induce fetal hemoglobin in human BFUe-derived colonies.
abstract: Established cell lines were screened for secretion of activities than can stimulate fetal hemoglobin (HbF) production in adult burst-forming unit-erythroid (BFUe) cultures. Conditioned media from four cell lines, a human teratocarcinoma, an osteosarcoma, a bladder cell carcinoma, and feline leukemia virus (FeLV) A-infected feline fibroblasts (FEF-A cells), consistently increased the relative production of fetal globin in BFUe-derived colonies. In vitro translation of RNA from these cells in Xenopus oocytes yielded products that increased the gamma to gamma+beta ratio in adult erythroid colonies. These results demonstrate that a variety of cell lines produce factors that stimulate the production of HbF in vitro. The genes of such factors could be isolated by expression cloning of cDNA from cell lines using the Xenopus oocyte system.
authors.affiliation: Department of Medicine, University of Washington, Seattle 98195.
authors.forename: P
authors.initials: P
authors.lastname: Constantoulakis
authors.forename: M
authors.initials: M
authors.lastname: Walmsley
authors.forename: R
authors.initials: R
authors.lastname: Patient
authors.forename: T
authors.initials: T
authors.lastname: Papayannopoulou
authors.forename: T
authors.initials: T
authors.lastname: Enver
authors.forename: G
authors.initials: G
authors.lastname: Stamatoyannopoulos
mesh_terms: D000818:Animals; D002415:Cats; D003470:Culture Media; D015672:Erythroid Precursor Cells / Q000378:metabolism*; D005319:Fetal Hemoglobin / Q000096:biosynthesis*; D005347:Fibroblasts / Q000378:metabolism* / Q000382:microbiology; D005914:Globins / Q000096:biosynthesis / Q000235:genetics; D006801:Humans; D005256:Leukemia Virus, Feline / Q000502:physiology; D051379:Mice; D009693:Nucleic Acid Hybridization; D009865:Oocytes / Q000378:metabolism; D012516:Osteosarcoma / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D013724:Teratoma / Q000378:metabolism*; D014162:Transfection; D001749:Urinary Bladder Neoplasms / Q000378:metabolism*; D014982:Xenopus laevis
chemical_list: D003470:Culture Media; D012333:RNA, Messenger; D005914:Globins; D005319:Fetal Hemoglobin