Functional dissection of AP-1 transcription factors using the Gal4 adaptor assay.
In the Gal4 adaptor assay, leucine zipper-containing proteins are tethered indirectly to the promoters of Gal4-responsive reporter genes via synthetic protein chimeras consisting of a Gal4 DNA-binding domain with an attached leucine zipper. Ternary complexes composed of the DNA binding site, the adaptor protein, and a leucine zipper factor can stimulate reporter gene activity, provided that the latter component possesses a transcriptional activation domain. This system is used to assay the transcriptional function and the interactions between various AP-1 factors.
Smith
S E
SE
European Molecular Biology Laboratory, Heidelberg, Germany.
Bohmann
D
D
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D002645:Chickens; D016762:Genes, fos; D016758:Genes, jun; D006801:Humans; D016350:Leucine Zippers; D008156:Luciferases / Q000096:biosynthesis / Q000235:genetics; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D015202:Protein Engineering / Q000379:methods*; D055503:Protein Multimerization; D016760:Proto-Oncogene Proteins c-fos / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D016755:Proto-Oncogene Proteins c-jun / Q000235:genetics* / Q000378:metabolism / Q000502:physiology*; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D014158:Transcription, Genetic; D001616:beta-Galactosidase / Q000235:genetics* / Q000378:metabolism
D016760:Proto-Oncogene Proteins c-fos; D016755:Proto-Oncogene Proteins c-jun; D011993:Recombinant Fusion Proteins; D008156:Luciferases; D001616:beta-Galactosidase