[Characteristics and biologic significance of changes in DNA content of rat liver cells during hepatocarcinogenesis].
DNA content of hepatocellular carcinoma (HCC) induced by diethylnitrosamine (DENA) in Wistar rats was quantitatively measured using flow cytometry. In normal adult rats, hepatocytes with tetraploid (4C) DNA content constitute the major cellular population (72.1%), whereas diploid (2C) hepatocytes were of a quite small number (16.9%). Under the persistent effect of DENA, the atavistic proliferation of 2C hepatocytes was obviously increased at the cirrhotic stage, which followed the pre-cirrhotic stage, and replaced 4C hepatocytes to become the major cellular population. HCC at its early cancerous stage was characterized by predominated of DNA 2C cells, and HCC at its progression stage showed a selective and advantageous growth of aneuploid(AN) stemline cells. The whole hepatocarcinogenesis course showed a change of DNA stemline from 4C to 2C to AN, which might be the essential biologic kinetic mechanism of the development and progression of HCC. It is also suggested that HCC at early and progression stages are characterized by predominance of DNA 2c and AN hepatocytes.
Cong
W M
WM
Second Military Medical University, Shanghai.
D000782:Aneuploidy; D000818:Animals; D004247:DNA / Q000032:analysis; D004273:DNA, Neoplasm / Q000032:analysis*; D004052:Diethylnitrosamine; D004171:Diploidy; D005434:Flow Cytometry; D008099:Liver / Q000737:chemistry; D008114:Liver Neoplasms, Experimental / Q000139:chemically induced / Q000235:genetics*; D008297:Male; D011230:Precancerous Conditions / Q000139:chemically induced / Q000235:genetics*; D051381:Rats
D004273:DNA, Neoplasm; D004052:Diethylnitrosamine; D004247:DNA