title: Ethylene dibromide: negative results with the mouse dominant lethal assay and the electrophoretic specific-locus test.
abstract: Ethylene dibromide (1,2-dibromoethane; EDB) was tested for the induction of dominant lethal and electrophoretically-detectable specific-locus mutations in the germ cells of DBA/2J male mice. Males were treated with a single intraperitoneal injection of 100 mg/kg EDB and mated to two C57BL/6J females. In the dominant lethal assay, matings were carried out to measure the effect of EDB on meiotic and postmeiotic stages; germ cells representing spermatogonial stem cells were analyzed in the electrophoretic specific-locus test. Neither of these germ cell tests produced any evidence that EDB is a germ cell mutagen. It appears from these data and those reported in the literature that EDB, a genotoxic carcinogen that affects male fertility in some mammalian species, is not mutagenic in the germ cells of the male mouse.
authors.affiliation: Research Triangle Institute, Research Triangle Park, NC 27709-2194.
authors.forename: L B
authors.initials: LB
authors.lastname: Barnett
authors.forename: D P
authors.initials: DP
authors.lastname: Lovell
authors.forename: C F
authors.initials: CF
authors.lastname: Felton
authors.forename: B J
authors.initials: BJ
authors.lastname: Gibson
authors.forename: R R
authors.initials: RR
authors.lastname: Cobb
authors.forename: D S
authors.initials: DS
authors.lastname: Sharpe
authors.forename: M D
authors.initials: MD
authors.lastname: Shelby
authors.forename: S E
authors.initials: SE
authors.lastname: Lewis
mesh_terms: D000818:Animals; D015946:Ethylene Dibromide / Q000633:toxicity*; D005298:Fertility / Q000187:drug effects; D005799:Genes, Dominant; D005804:Genes, Lethal; D008297:Male; D051379:Mice; D009152:Mutagenicity Tests
chemical_list: D015946:Ethylene Dibromide