[Changes in activity of phospholipase A2 and its metabolic production of prostaglandins in lung tissue of asthmatic guinea pigs].
The activity of phospholipase A2 and its metabolic production-prostaglandins were examined in lung tissue of allergic asthmatic guinea pigs. The results shows that the activity of phospholipase A2 after three days of asthma attack was raised 54.19% than that of control group (P < 0.01), and decreased 69.27% when protected with chloroquine before asthma, and decreased 65.98% when only given chloroquine as a control; the ratio of those prostaglandins was resumed to control group when previously given chloroquine in asthma and normal guinea pigs. These results suggest that the activity of phospholipase A2 and changes of prostaglandins in lung tissue were related to allergic asthma of guinea pigs, the increase of TXB2 or PGF2 alpha and decrease of PGE2 or 6-Keto-PGF2 alpha in lung tissue after three days of asthma attack may have a correlation with allergic asthma in guinea pigs.
Yan
G T
GT
Laboratory of Truma Center, 304 Hospital of PLA, Beijing.
D015121:6-Ketoprostaglandin F1 alpha / Q000378:metabolism; D000418:Albumins; D000818:Animals; D001249:Asthma / Q000139:chemically induced / Q000201:enzymology* / Q000378:metabolism; D002738:Chloroquine / Q000008:administration & dosage; D015237:Dinoprost / Q000378:metabolism; D015232:Dinoprostone / Q000378:metabolism; D006168:Guinea Pigs; D008168:Lung / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism; D008297:Male; D010741:Phospholipases A / Q000378:metabolism*; D054467:Phospholipases A2; D011453:Prostaglandins / Q000378:metabolism*
D000418:Albumins; D011453:Prostaglandins; D015121:6-Ketoprostaglandin F1 alpha; D002738:Chloroquine; D015237:Dinoprost; D010741:Phospholipases A; D054467:Phospholipases A2; D015232:Dinoprostone