Renal tubular epithelial antigen-containing immune complexes stimulate interleukin-1 production by monocytes from patients with glomerulonephritis.
We have investigated the effect of immune complexes (IC) derived from human renal tubular epithelial (RTE) antigen on the release of interleukin-1 (IL-1) in monocyte cultures from patients with glomerulonephritis (GN). When peripheral blood monocytes (PBM) were activated by IC, substantial amounts of IL-1 could be detected in the supernatants as measured by mouse thymocyte assay. The IC-induced IL-1 activity was significantly higher in patients with GN than in normal controls. To avoid the effect of prostaglandins on the IL-1 assay, we cultured PBM with addition of indomethacin and assayed IL-1 activity in the culture supernatants. This cyclooxygenase inhibitor augmented IC-induced IL-1 production. The results suggest that IC are involved in stimulating IL-1 production by PBM and thus play a role in the immune response in GN.
Matsumoto
K
K
Second Department of Internal Medicine, Nihon University School of Medicine, Tokyo, Japan.
D000293:Adolescent; D000328:Adult; D000936:Antigen-Antibody Complex / Q000276:immunology*; D000941:Antigens / Q000276:immunology*; D005260:Female; D005921:Glomerulonephritis / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D007213:Indomethacin / Q000494:pharmacology; D007375:Interleukin-1 / Q000096:biosynthesis*; D007684:Kidney Tubules / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism*
D000936:Antigen-Antibody Complex; D000941:Antigens; D007375:Interleukin-1; C035812:renal tubular antigen; D007213:Indomethacin