DNA polymorphism studies. Approaches to elucidating multifactorial ischaemic heart disease: the apo B gene as an example.
It is well established that elevated plasma levels of low-density lipoprotein (LDL) particles are a risk factor for ischaemic heart disease with the distribution in LDL levels seen in the general population being the result of interaction between environmental factors, such as dietary fat intake, and genetic variation that is present in different individuals. One of the candidate genes where such variation is likely to occur, is the gene coding for apolipoprotein B (apo B). Many studies have reported an association between a common polymorphism of the apo B gene, detected using the restriction enzyme XbaI, and differences in plasma lipid levels, explaining 3-5% of the variance in LDL-cholesterol levels in samples representative of the healthy population. It has been proposed that the mechanism of this association is due to functional amino acid changes within the apo B protein, that affect LDL catabolism by altering binding affinity to the LDL-receptor. Several amino acid substitutions in the apo B gene have now been characterized, and these form the basis of the different epitopes that create the Ag marker system. Previous studies have reported that the Ag(x) epitope is associated with lower plasma lipid levels, and until recently the molecular basis for this association has been unclear. We have determined that the Ag(x) epitope is associated with both a Pro-Leu2712, and Asn-Ser4311 substitution, with the Leu-Ser allele being associated with significantly lower levels of plasma lipids in a sample of healthy individuals from Sweden.(ABSTRACT TRUNCATED AT 250 WORDS)
Humphries
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SE
Centre for Cardiovascular Genetics, Department of Medicine, Rayne Institute, London, UK.
Dunning
A
A
Xu
C F
CF
Peacock
R
R
Talmud
P
P
Hamsten
A
A
D000483:Alleles; D001055:Apolipoproteins B / Q000097:blood / Q000235:genetics*; D002784:Cholesterol / Q000097:blood; D003327:Coronary Disease / Q000097:blood / Q000209:etiology / Q000235:genetics*; D004247:DNA / Q000235:genetics*; D000939:Epitopes; D005796:Genes; D005838:Genotype; D006801:Humans; D006949:Hyperlipidemias / Q000235:genetics; D008055:Lipids / Q000097:blood; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D012307:Risk Factors
D001055:Apolipoproteins B; D000939:Epitopes; D008055:Lipids; D004247:DNA; D002784:Cholesterol