[Determination of antigen-dependent activity of human lung cancer transfer factor by H3-leucine leucine leukocyte adherence inhibition assay].
This report is to demonstrate the antigen-dependent activity of human lung cancer transfer factor (Sp-TF). Sp-TFM was prepared from spleen of mice immunized with the human lung cancer cell line A549. [3H]-leu leukocyte adherence inhibition assay ([3H]-leu-LAI) was modified to identify activity of Sp-TFM. Leukocytes obtained from non-immunized mice were divided into eight groups as follows: 1. Control without TF or antigen; 2. Sp-TFM and antigen of cell line A549 (A549 Ag); 3. Sp-TFM alone; 4. Sp-TFM and ascitic tumor cell H22 antigen of mice (H22Ag); 5. Sp-TFM and antigen of human gastric cancer cell (HGCCAg); 6. Sp-TFM and antigen of human normal lung tissue (NLTAg); 7. Nor special TF of mice (N-TFM) and A549Ag; 8. A549Ag alone. When normal leukocytes were incubated with Sp-TFM and A549 antigen, the leukocytes adherence inhibition index (LAII) was significantly higher than those of the other groups. The different LAII of Sp-TFM to A549Ag and other experimental groups were highly significant (P less than 0.001). The results demonstrated that Sp-TFM could transfer specific cell mediated immunity to non-immune leukocytes. The TF prepared from spleen of goat immunized with antigen from lung cancer of patients (Sp-TFG) showed antigen specific activity as well as Sp-TFM.
Xu
W M
WM
Fourth Military Medical University, Xian, Shanxi.
D000818:Animals; D000951:Antigens, Neoplasm / Q000276:immunology*; D000939:Epitopes; D006801:Humans; D007957:Leukocyte Adherence Inhibition Test; D008175:Lung Neoplasms / Q000276:immunology* / Q000473:pathology; D008297:Male; D051379:Mice; D014165:Transfer Factor / Q000096:biosynthesis* / Q000276:immunology; D014407:Tumor Cells, Cultured
D000951:Antigens, Neoplasm; D000939:Epitopes; D014165:Transfer Factor