Improving lactate analysis with the YSI 2300 GL: hemolyzing blood samples makes results comparable with those for deproteinized whole blood.
To obviate the well-documented problem of hematocrit dependency of the Yellow Springs Instruments (YSI) whole-blood lactate analyzer, we modified the dilution buffer by including a lysing reagent. This makes the results comparable with those of methods performed with deproteinized whole-blood samples. No centrifugation step is needed, thus preserving the convenience of the YSI instrument for stat and field use. The modification works equally well on plasma samples. Lactate concentrations measured in nonhemolyzed whole blood are not comparable with results for hemolyzed whole blood or protein-precipitated whole blood. We therefore recommend to all users of YSI equipment to lyse the erythrocytes before lactate determinations.
Foxdal
P
P
Department of Clinical Physiology, University Hospital, Uppsala, Sweden.
Bergqvist
Y
Y
Eckerbom
S
S
Sandhagen
B
B
D015374:Biosensing Techniques / Q000592:standards; D001774:Blood Chemical Analysis / Q000295:instrumentation* / Q000592:standards; D004355:Drug Stability; D006461:Hemolysis; D006801:Humans; D007202:Indicators and Reagents; D007773:Lactates / Q000097:blood*; D019344:Lactic Acid; D013696:Temperature; D013997:Time Factors
D007202:Indicators and Reagents; D007773:Lactates; D019344:Lactic Acid