Induction of peroxisomal enzymes and a 64-kDa peptide in cultured mouse macrophages treated with clofibrate.
The presence of catalase-containing particles was demonstrated in mouse macrophages of the cell line J774.3. These cells were incubated with clofibrate, a hypolipidemic drug known to cause peroxisome proliferation in rodent hepatocytes. The specific activities of catalase and fatty acid oxidase were significantly increased after 48 h incubation with 2 mM clofibrate. This response proved to be time and dose dependent. Other organelle marker enzymes showed little change in activity. Unexpectedly, clofibrate treatment also produced an increase in a 64-kDa peptide in these cells.
Couve
A O
AO
Department of Cell and Molecular Biology, Pontificia Universidad Católica de Chile, Santiago.
Koenig
C
C
Santos
M J
MJ
D000818:Animals; D002460:Cell Line; D002994:Clofibrate / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel; D004790:Enzyme Induction / Q000187:drug effects; D005455:Fluorescent Antibody Technique; D007700:Kinetics; D008264:Macrophages / Q000187:drug effects / Q000201:enzymology*; D051379:Mice; D008830:Microbodies / Q000201:enzymology*
D002994:Clofibrate