title: The influence of neuraminidase treatment on tracheal smooth muscle contraction.
abstract: To examine the role of sialic acid in the respiratory tract, the influence of neuraminidase from Clostridium perfringens was investigated on contractions of isolated guinea-pig and rat trachea and on histamine release from guinea-pig lung tissue. Treatment with 2.0 units/ml of neuraminidase at 37 degrees C and pH 7.4 for 30 min caused an approximately 60% removal of total N-acetylneuraminic acid, a representative sialic acid, from muscle from guinea-pig and rat trachea. Neuraminidase concentration dependently induced histamine release from guinea-pig chopped lung tissue, but has no effect on contractions produced by acetylcholine, histamine and 5-hydroxytryptamine. Pretreatment with 2.0 units/ml of neuraminidase inhibited the contraction induced by antigen (ovalbumin) or compound 48/80. These findings suggest, at least in part, that sialic acids sensitive to neuraminidase are involved in the regulation of histamine release but not tracheal contraction.
authors.affiliation: Department of Pharmacological Sciences, Faculty of Pharmaceutical Sciences, Kumamoto University, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Kai
authors.forename: K
authors.initials: K
authors.lastname: Makise
authors.forename: S
authors.initials: S
authors.lastname: Matsumoto
authors.forename: T
authors.initials: T
authors.lastname: Ishii
authors.forename: K
authors.initials: K
authors.lastname: Takahama
authors.forename: Y
authors.initials: Y
authors.lastname: Isohama
authors.forename: T
authors.initials: T
authors.lastname: Miyata
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D006168:Guinea Pigs; D006632:Histamine / Q000494:pharmacology; D006636:Histamine Release / Q000187:drug effects; D008168:Lung / Q000187:drug effects / Q000378:metabolism; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects; D009130:Muscle, Smooth / Q000187:drug effects* / Q000502:physiology; D019158:N-Acetylneuraminic Acid; D009439:Neuraminidase / Q000494:pharmacology*; D010047:Ovalbumin / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D012701:Serotonin / Q000494:pharmacology; D012794:Sialic Acids / Q000502:physiology*; D014132:Trachea / Q000187:drug effects* / Q000502:physiology; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology
chemical_list: D012794:Sialic Acids; D012701:Serotonin; D003189:p-Methoxy-N-methylphenethylamine; D006632:Histamine; D010047:Ovalbumin; D009439:Neuraminidase; D019158:N-Acetylneuraminic Acid; D000109:Acetylcholine