Machine perfusion of isolated kidney at 37 degrees C using pyridoxalated hemoglobin-polyoxyethylene (PHP) solution, UW solution and its combination.
To preserve isolated kidney normothermically, PHP containing UW components were evaluated as perfusates. Kidneys were flushed out by Lactate Ringer solution immediately after isolation from mongrel dogs, and then connected to the perfusion circuit which consists of a preservation box, a reservoir of perfusate, a membrane oxygenator and a drive unit. PHP containing 140 mEq/l of Na+ and 4 mEq/l of K+ (PHP(E)), UW solution (UW) and UW components added PHP(E) were prepared and adjusted at pH 7.4 prior to use. Temperature and perfusion pressure were controlled at 37 degrees C and 100 mmHg, respectively. During 12 hour perfusion, remarkable changes in pH were seen in UW group and PHP group while higher oxygen consumption was noted in PHP(E)+UW group than that in PHP(E) group. The histological findings showed moderate damages of tubular epithelial cells and maintaining normal glomerular structure in PHP(E)+UW while severe damage of both tubulus in UW group were seen. There was no edematous degeneration in both UW and PHP(E)+UW groups, however, it was seen in PHP(E) alone. It was suggested that components of UW solution have positive effect on normothermic machine perfusion with PHP(E) solution.
Horiuchi
T
T
Department of Precision Machinery Engineering, Faculty of Engineering, University of Tokyo, Japan.
Ohta
Y
Y
Hashimoto
K
K
Yamaguchi
N
N
Dohi
T
T
Uechi
M
M
Watanabe
T
T
D000241:Adenosine; D000493:Allopurinol; D000818:Animals; D001802:Blood Substitutes; D004285:Dogs; D005069:Evaluation Studies as Topic; D005978:Glutathione; D006454:Hemoglobins; D066298:In Vitro Techniques; D007328:Insulin; D007668:Kidney / Q000033:anatomy & histology / Q000502:physiology; D009926:Organ Preservation / Q000295:instrumentation / Q000379:methods*; D019188:Organ Preservation Solutions; D010101:Oxygen Consumption; D010477:Perfusion; D011092:Polyethylene Glycols; D011887:Raffinose; D012996:Solutions; D013696:Temperature; D013997:Time Factors
D001802:Blood Substitutes; D006454:Hemoglobins; D007328:Insulin; D019188:Organ Preservation Solutions; D012996:Solutions; C055206:University of Wisconsin-lactobionate solution; C050907:pyridoxalated-hemoglobin-polyethylene glycol conjugate; D011092:Polyethylene Glycols; D000493:Allopurinol; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose