Antibody response to human papovavirus JC (JCV) and simian virus 40 (SV40) T antigens in SV40 T antigen-transgenic mice.
Human papovavirus JC (JCV) and simian virus 40 (SV40) genomes share approximately 69% homology; and there is antigenic cross-reactivity between JCV and SV40 tumor or T antigens. In order to determine whether a selective immune response to JCV T antigen could be demonstrated, transgenic mice (SV11+) that express SV40 T antigen in the choroid plexus and are partially tolerant to antigenic determinants on SV40 T antigen were immunized with SV40 or JCV T antigens and their antibody responses were analyzed. The results show that SV11+ mice responded as well as their nontransgenic litter mates to JCV T antigen. Monoclonal antibodies were derived from hybridomas generated from immunized mice which reacted specifically with epitopes in the amino and carboxy terminal halves on JCV T antigen. These studies show that transgenic mice expressing SV40 T antigen are capable of responding to determinants not shared between JCV and SV40 T antigen.
Tevethia
S S
SS
Department of Microbiology and Immunology, Pennsylvania State University College of Medicine, Hershey 17033.
Epler
M
M
Georgoff
I
I
Teresky
A
A
Marlow
M
M
Levine
A J
AJ
D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology*; D000952:Antigens, Polyomavirus Transforming / Q000276:immunology*; D000939:Epitopes / Q000276:immunology; D007577:JC Virus / Q000276:immunology*; D051379:Mice; D008822:Mice, Transgenic; D011233:Precipitin Tests
D000914:Antibodies, Viral; D000952:Antigens, Polyomavirus Transforming; D000939:Epitopes