Determination of AJ-3941, a possible agent for the treatment of cerebrovascular disorders, in plasma and brain by means of high-performance liquid chromatography with fluorescence detection.
A sensitive and selective high-performance liquid chromatographic method with fluorescence detection is described for the determination of AJ-3941 (I), a possible agent for the treatment of cerebrovascular disorders, in plasma and brain tissue. A simple hexane extraction was used for plasma, and for brain homogenate the hexane extract was further purified by solid-phase extraction. The determination limit was ca. 3 ng/ml for both plasma (0.5 ml) and 10% (w/v) brain homogenate (1 ml). The method was applied to the determination of I in plasma and brain samples of experimental animals.
Kurono
M
M
Exploratory Research Laboratories, Dainippon Pharmaceutical Co., Ltd., Osaka, Japan.
Yoshida
K
K
Naruto
S
S
D000818:Animals; D001923:Brain Chemistry; D002561:Cerebrovascular Disorders / Q000097:blood / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003990:Dibenzoxepins / Q000032:analysis* / Q000097:blood / Q000493:pharmacokinetics; D004285:Dogs; D006586:Hexanes; D051379:Mice; D010879:Piperazines / Q000032:analysis* / Q000097:blood / Q000493:pharmacokinetics; D051381:Rats
D003990:Dibenzoxepins; D006586:Hexanes; D010879:Piperazines; C077479:AJ 3941; C026385:n-hexane