Pentraxin family of proteins interact specifically with phosphorylcholine and/or phosphorylethanolamine.
Pentraxins are a family of serum proteins characterized by five identical subunits that are noncovalently linked. The two major types of pentraxins are C-reactive protein (CRP) and serum amyloid P component (SAP). CRP proteins are identified by their calcium-dependent interaction with phosphorylcholine. This study showed that SAP also bound to phosphorylated compounds but had a high specificity for phosphorylethanolamine. Thus, human CRP and SAP show high specificity that is complementary for the related compounds, phosphorylcholine and phosphorylethanolamine, respectively. This relationship suggests a complementary and/or related function for the pentraxins. Pentraxins from other species were also examined. Mouse SAP showed binding interactions and specificity similar to human SAP. Female protein (FP) from hamster and rat CRP showed a hybrid specificity and bound to both phosphorylethanolamine and phosphorylcholine. All of the proteins that bound phosphorylethanolamine also associated with human C4b-binding protein (C4BP). With the exception of human and rat CRP, all the proteins also bound to vesicles containing acidic phospholipids. All of these binding interactions were calcium-dependent and mutually exclusive, suggesting that they involved the same site on the protein. These findings suggest possible ways to examine the function of the pentraxins.
Schwalbe
R A
RA
Department of Biochemistry, University of Minnesota, St. Paul 55108.
Dahlbäck
B
B
Coe
J E
JE
Nelsestuen
G L
GL
D000510:Alpha-Globulins / Q000737:chemistry*; D000818:Animals; D002097:C-Reactive Protein / Q000737:chemistry*; D002352:Carrier Proteins / Q000737:chemistry; D006224:Cricetinae; D004983:Ethanolamines / Q000737:chemistry*; D005260:Female; D006801:Humans; D016167:Integrin alphaXbeta2; D046911:Macromolecular Substances; D008647:Mesocricetus; D051379:Mice; D008823:Micelles; D010710:Phosphates / Q000737:chemistry; D010767:Phosphorylcholine / Q000737:chemistry*; D051381:Rats; D000683:Serum Amyloid P-Component / Q000737:chemistry*; D013329:Structure-Activity Relationship
D000510:Alpha-Globulins; D002352:Carrier Proteins; D004983:Ethanolamines; D016167:Integrin alphaXbeta2; D046911:Macromolecular Substances; D008823:Micelles; D010710:Phosphates; D000683:Serum Amyloid P-Component; C030974:female protein, hamster; D010767:Phosphorylcholine; C005448:phosphorylethanolamine; D002097:C-Reactive Protein