Characterization of murine hemopoietic-supportive (MS-1 and MS-5) and non-supportive (MS-K) cell lines.
Characteristics of hemopoietic-supportive (MS-1 and MS-5) and non-supportive (MS-K) cell lines were compared. Supportive cells adhered to hemopoietic stem cells and produced granulocyte-macrophage colony-stimulating factor (GM-CSF), whereas non-supportive cells did not adhere to hemopoietic cells and only produced macrophage colony-stimulating factor. Both cell lines produced substantial levels of IL-6 and steel factor (SLF) which is reportedly a stem-cell factor. Northern blot analysis revealed that SLF but neither c-kit nor interleukin 3 (IL-3) mRNA was detectable in these cell lines, although IL-3-like activity was found in the supernatant of MS-5 cell culture. These observations suggest that the hemopoietic-supportive function of stromal cells may reside in adherence of stem cells, and production of GM-CSF probably in combination with SLF. SLF may be transferred from stromal cells directly to stem cells through adhesion of stem cells to supportive stromal cells.
Suzuki
J
J
Faculty of Science, Niigata University, Japan.
Fujita
J
J
Taniguchi
S
S
Sugimoto
K
K
Mori
K J
KJ
D000818:Animals; D002448:Cell Adhesion; D002460:Cell Line; D002470:Cell Survival; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000096:biosynthesis*; D016298:Hematopoietic Cell Growth Factors / Q000096:biosynthesis / Q000494:pharmacology; D006412:Hematopoietic Stem Cells / Q000187:drug effects / Q000378:metabolism*; D015850:Interleukin-6 / Q000096:biosynthesis / Q000494:pharmacology; D051379:Mice; D012397:Rosette Formation; D019089:Stem Cell Factor
D016298:Hematopoietic Cell Growth Factors; D015850:Interleukin-6; D019089:Stem Cell Factor; D016178:Granulocyte-Macrophage Colony-Stimulating Factor