title: Infectivity of human T-lymphotropic virus type I to human nervous tissue cells in vitro.
abstract: Infectivity of human T-lymphotropic virus type I (HTLV-I) to human nervous tissue cells was explored using co-cultivation with X-irradiated, HTLV-I-producing MT2 cells. Examined cells included normal cerebellar cells, brain tumor cells (astrocytoma, medulloblastoma, meningioma, hemangioblastoma, and schwannoma), and various cell lines (astrocytoma, ependymoma, oligodendroglioma, medulloblastoma, and neuroblastoma). Successful HTLV-I infection was confirmed immunohistochemically using monoclonal antibodies to HTLV-I p19, p24, and pX product. All cell lines and primary cultures from normal cerebellar tissues and brain tumors could be infected with HTLV-I. Double immunostaining showed that glial fibrillary acidic protein-, S-100 protein- or vimentin-positive cells were susceptible to infection. Neurofilament- or neuron-specific enolase-positive cells in medulloblastoma could also be infected. Reverse-transcriptase assay revealed the productive infection in U251-MG (astrocytoma) and KG-IC (oligodendroglioma) lines. Co-cultivated U251-MG cells formed syncytial polykaryons after serial passages, and polymerase chain reaction assay detected HTLV-I genome in U251-MG syncytial polykaryons and p19+ mononuclear cells. HTLV-I viral RNA was also detected in infected U251-MG cells by in situ hybridization. These data show that HTLV-I may have a wide spectrum of infectivity in human nervous tissues.
authors.affiliation: Department of Pathology, Okayama University Medical School, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Akagi
authors.forename: Y
authors.initials: Y
authors.lastname: Hoshida
authors.forename: T
authors.initials: T
authors.lastname: Yoshino
authors.forename: N
authors.initials: N
authors.lastname: Teramoto
authors.forename: E
authors.initials: E
authors.lastname: Kondo
authors.forename: K
authors.initials: K
authors.lastname: Hayashi
authors.forename: K
authors.initials: K
authors.lastname: Takahashi
mesh_terms: D001483:Base Sequence; D001932:Brain Neoplasms / Q000382:microbiology; D002460:Cell Line; D004279:DNA, Viral / Q000032:analysis; D015368:Human T-lymphotropic virus 1 / Q000472:pathogenicity*; D006801:Humans; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D009417:Nerve Tissue / Q000382:microbiology*; D009693:Nucleic Acid Hybridization; D015493:Paraparesis, Tropical Spastic / Q000382:microbiology / Q000473:pathology*; D016133:Polymerase Chain Reaction; D012194:RNA-Directed DNA Polymerase / Q000032:analysis
chemical_list: D004279:DNA, Viral; D012194:RNA-Directed DNA Polymerase