Mitomycin C, 4-nitroquinoline-1-oxide and ethyl methanesulfonate induce long-lived lesions in DNA which result in SCEs during successive cell cycles in human lymphocytes.
The present study was carried out in order to analyze how persistent the lesions in DNA are which elicit sister-chromatid exchanges (SCEs), induced by three different chemical agents, mitomycin C (MMC), 4-nitroquinoline-1-oxide (4NQO) and ethyl methanesulfonate (EMS), in proliferating human lymphocytes. Cells were exposed to the mutagens for 1 h just before starting bromodeoxyuridine substitution and SCEs were examined in third-cycle metaphases showing three-way-differential staining, by means of our previously standardized method. The results show that, in spite of the fact that these three compounds have different modes of action, the lesions induced by all of them seem to be capable of persisting in DNA and eliciting SCEs for at least three successive cell cycles.
Daza
P
P
Departamento de Biología Celular, Facultad de Biologia, Universidad de Sevilla, Spain.
Escalza
P
P
Mateos
S
S
Cortés
F
F
D015112:4-Nitroquinoline-1-oxide / Q000633:toxicity*; D002453:Cell Cycle; D004247:DNA / Q000187:drug effects*; D004249:DNA Damage; D005020:Ethyl Methanesulfonate / Q000633:toxicity*; D006801:Humans; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects; D016685:Mitomycin / Q000633:toxicity*; D016296:Mutagenesis; D009153:Mutagens / Q000633:toxicity*; D012854:Sister Chromatid Exchange; D013997:Time Factors
D009153:Mutagens; D016685:Mitomycin; D015112:4-Nitroquinoline-1-oxide; D004247:DNA; D005020:Ethyl Methanesulfonate