title: Changes in intracellular and cell surface localization of Le(x) epitope during germ cell differentiation in fetal mice.
abstract: Changes in the intracellular and cell surface localization of Lewis x (Le(x)) determinants in germ cells during fetal development in mice were examined by light and electron microscopy. Light microscopically, in undifferentiated gonads on day 12 post coitum (p.c.), the anti-Le(x) monoclonal antibody (MAb) was specifically bound to the plasma membranes and to the cytoplasmic granule-like structures of germ cells. In the testes on day 13 p.c., most of the germ cells were enclosed within the testicular cords and showed an MAb-positive reaction which was restricted mainly to the cytoplasmic granule-like structures. The reaction on the plasma membranes almost disappeared. On the other hand, the ectopic germ cells still showed a positive reaction on their plasma membranes. In the ovaries on day 13 p.c., the germ cells also exhibited positive reactions both on the plasma membranes and on the granule-like structures. Immunoelectron microscopic observations agreed well with these light microscopic observations in such a way that both the plasma membranes and the "small dense bodies" (SDB) were positive in undifferentiated gonads on day 12 p.c. In the germ cells organized into the testicular cords, the reaction to anti-Le(x) MAb became restricted to the SDB. These results may indicate that such intracellular changes in Le(x) determinants during germ cell differentiation are associated with the enclosure of germ cells within the testicular cords.
authors.affiliation: Department of Veterinary Anatomy, Faculty of Agriculture, University of Tokyo, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Kanai
authors.forename: H
authors.initials: H
authors.lastname: Kawakami
authors.forename: M
authors.initials: M
authors.lastname: Kanai-Azuma
authors.forename: M
authors.initials: M
authors.lastname: Kurohmaru
authors.forename: H
authors.initials: H
authors.lastname: Hirano
authors.forename: Y
authors.initials: Y
authors.lastname: Hayashi
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000954:Antigens, Surface / Q000032:analysis; D002454:Cell Differentiation; D000939:Epitopes / Q000032:analysis; D005260:Female; D005854:Germ Cells / Q000166:cytology / Q000276:immunology* / Q000648:ultrastructure; D007150:Immunohistochemistry; D016256:Lewis X Antigen / Q000032:analysis*; D008297:Male; D051379:Mice; D010053:Ovary / Q000196:embryology* / Q000276:immunology / Q000648:ultrastructure; D012733:Sex Differentiation; D013737:Testis / Q000196:embryology* / Q000276:immunology / Q000648:ultrastructure
chemical_list: D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D000939:Epitopes; D016256:Lewis X Antigen