Inclusion and fractionated release of nucleic acids using microcapsules made from plant cells.
The encapsulation and fractionated release of nucleic acids on vesicular packing (VP) materials have been investigated. The earlier described dependence of the permeation of nucleic acid molecules through the vesicle membranes on the salt concentration is a necessary precondition for both encapsulation and fractionation. Encapsulation is achieved by applying a suitable sample onto a VP column that has been equilibrated with a high-salt buffer. In that buffer the sample molecules are permeable. Immediately after sample application, elution is started with a low-salt buffer, from which the sample molecules are excluded. At the front between the two buffers the permeability changes, and some of the sample molecules distributed inside the vesicles cannot pass through the membranes. These encapsulated molecules can be released by increasing the salt concentration in the eluent. If the encapsulated nucleic acid sample is polydisperse, a stepwise or linear increase in the salt concentration leads to a fractionated release. The fractions obtained differ in their molecular size composition.
Jäschke
A
A
Department of Chemistry, Humboldt University, Berlin, Germany.
Cech
D
D
Ehwald
R
R
D001483:Base Sequence; D002021:Buffers; D002214:Capsules; D002463:Cell Membrane Permeability / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D015636:Magnesium Chloride / Q000494:pharmacology; D008969:Molecular Sequence Data; D009696:Nucleic Acids / Q000378:metabolism*; D059828:Plant Cells; D012965:Sodium Chloride / Q000494:pharmacology
D002021:Buffers; D002214:Capsules; D009696:Nucleic Acids; D015636:Magnesium Chloride; D012965:Sodium Chloride