Co-ordinate expression of c-fos, p53 and cytokeratin genes during the alteration of growth of human hepatoma cells. mRNA levels measured by reverse transcription and polymerase chain reaction.
Teleocidin, a tumor promoter, inhibited the proliferation, enhanced cytokeratin assembly and increased the type III procollagen production of PLC/PRF/5 hepatoma cells. Teleocidin transiently increased the levels of c-fos and p53 mRNAs measured by reverse transcription and polymerase chain reaction. This was followed by a reduction of c-myc mRNA and an increase of cytokeratin mRNA. The level of p120 mRNA was not remarkably altered. Sequential alterations of the expression of c-fos, p53, c-myc and cytokeratin genes induced by teleocidin may be responsible for the morphological and functional changes of hepatoma cells induced by this tumor promoter.
Kaneko
Y
Y
First Department of Medicine, University of Tokyo, Faculty of Medicine, Japan.
Tsukamoto
A
A
Korokawa
K
K
D001483:Base Sequence; D006528:Carcinoma, Hepatocellular / Q000378:metabolism* / Q000473:pathology; D015870:Gene Expression; D016762:Genes, fos; D016259:Genes, myc; D016158:Genes, p53; D006801:Humans; D007633:Keratins / Q000235:genetics*; D008113:Liver Neoplasms / Q000378:metabolism* / Q000473:pathology; D008235:Lyngbya Toxins / Q000494:pharmacology; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis*; D012194:RNA-Directed DNA Polymerase / Q000235:genetics; D014407:Tumor Cells, Cultured
D008235:Lyngbya Toxins; D012333:RNA, Messenger; C022176:teleocidins; D007633:Keratins; D012194:RNA-Directed DNA Polymerase