title: Regulation of the supramolecular structure and the catalytic activity of penicillin acylase from Escherichia coli in the system of reversed micelles of Aerosol OT in octane.
abstract: The properties of penicillin acylase from E. coli solubilized by hydrated reversed micelles (RM) of Aerosol OT in octane were studied. The dependence of catalytic activity on the hydration degree, a parameter which determines the size of the micelle inner cavity, has a curve with three optima, each one corresponding to the enzyme functioning either in a dimer form (wo = 23) or in a form of separate subunits, a heavy one, beta, and a light one, alpha (wo = 20 and 14, respectively). The reversible dissociation of the enzyme was confirmed by ultracentrifugation followed by electrophoresis.
authors.affiliation: Department of Chemical Enzymology, Moscow State University, Russia.
authors.forename: V E
authors.initials: VE
authors.lastname: Kabakov
authors.forename: S
authors.initials: S
authors.lastname: Merker
authors.forename: N L
authors.initials: NL
authors.lastname: Klyachko
authors.forename: K
authors.initials: K
authors.lastname: Martinek
authors.forename: A V
authors.initials: AV
authors.lastname: Levashov
mesh_terms: D004143:Dioctyl Sulfosuccinic Acid; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000201:enzymology*; D007700:Kinetics; D046911:Macromolecular Substances; D008823:Micelles; D009795:Octanes / Q000494:pharmacology; D010399:Penicillin Amidase / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D010664:Phenylmethylsulfonyl Fluoride / Q000494:pharmacology; D013501:Surface-Active Agents
chemical_list: D046911:Macromolecular Substances; D008823:Micelles; D009795:Octanes; D013501:Surface-Active Agents; D004143:Dioctyl Sulfosuccinic Acid; D010664:Phenylmethylsulfonyl Fluoride; D010399:Penicillin Amidase; C026728:octane