Rod opsin cDNA sequence from the sand goby (Pomatoschistus minutus) compared with those of other vertebrates.
The absorbance spectra of rods from the sand goby were measured by using microspectrophotometry. Analysis of the averaged spectra shows that the rod visual pigment has a maximum absorbance (lambda max) at approximately 501 nm. A sand goby retinal cDNA library was constructed and then screened with a partial sand goby rod opsin clone obtained by the polymerase chain reaction (PCR). The screening of the library yielded a full length rod opsin clone. The cDNA sequence and deduced amino acid sequence of this clone are compared with those of other vertebrate rod opsins.
Archer
S N
SN
Department of Biochemistry, University of Bristol, U.K.
Lythgoe
J N
JN
Hall
L
L
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002645:Chickens; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D005136:Eye Proteins / Q000235:genetics*; D005399:Fishes / Q000235:genetics*; D015723:Gene Library; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D010786:Photoreceptor Cells / Q000502:physiology*; D016133:Polymerase Chain Reaction / Q000379:methods; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012160:Retina / Q000502:physiology; D017299:Rod Opsins; D012689:Sequence Homology, Nucleic Acid
D005136:Eye Proteins; D009838:Oligodeoxyribonucleotides; D017299:Rod Opsins; D012313:RNA; D004247:DNA