The involvement of protein kinase C in activation-induced cell death in T-cell hybridoma.
T-cell hybridoma activated by a variety of stimuli such as anti-cell surface antigen, notably CD3 and T-cell receptors, and Con A undergoes a cell lysis process called activation-induced cell death (AICD). It was found that the major protein kinase C (PKC) isoform in the 2B4.11 T-cell hybridoma, PKC(alpha), was translocated from the cytosolic to the particulate fraction when these hybridoma cells were induced to die by plastic-adsorbed anti-CD3 antibodies. Inhibitors of protein phosphorylation rescued 2B4.11 cells from AICD as determined by the analysis of cellular metabolism and the proportion of living cells. Furthermore, PKC(alpha) down-regulation by phorbol ester treatment abolished AICD, and the degree of PKC down-regulation correlated well with the degree of AICD abolishment, suggesting that PKC activation represents an essential step in the molecular mechanisms underlying AICD in this T-cell hybridoma.
Jin
L W
LW
Department of Neurosciences, School of Medicine, University of California, San Diego, La Jolla 92093-0624.
Inaba
K
K
Saitoh
T
T
D000470:Alkaloids / Q000494:pharmacology*; D000911:Antibodies, Monoclonal; D017252:CD3 Complex; D016923:Cell Death / Q000187:drug effects; D016572:Cyclosporine / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation / Q000187:drug effects; D019833:Genistein; D006825:Hybridomas / Q000276:immunology; D007529:Isoflavones / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000276:immunology*; D010703:Phorbol Esters / Q000494:pharmacology; D011493:Protein Kinase C / Q000276:immunology*; D019311:Staurosporine; D013601:T-Lymphocytes / Q000276:immunology*
D000470:Alkaloids; D000911:Antibodies, Monoclonal; D017252:CD3 Complex; D007529:Isoflavones; D010703:Phorbol Esters; D016572:Cyclosporine; D019833:Genistein; D011493:Protein Kinase C; D019311:Staurosporine