Determination of protein pattern in embryonic cavities of human early pregnancies: a means to understand materno-embryonic exchanges.
Exocoelomic and amniotic fluids were obtained by selective puncture under ultrasound guidance in normal human pregnancies between 5 and 13 weeks of gestation. Evaluation of the protein patterns in the exocoelomic fluid showed qualitative and quantitative changes with advancing gestation. During the second month of gestation, three electrophoretic bands were found with mobility compatible with albumin, alpha 1-globulin and beta-globulin and composed of at least eight proteins including: pre-albumin, albumin, alpha-fetoprotein (alpha-FP), alpha 1-protease inhibitor, haptoglobin, ceruloplasmin, transferrin and immunoglobulin-G, as revealed by immunoblotting. Protein patterns obtained between 9 and 13 weeks were comparable in exocoelomic fluid and in maternal serum except for the presence of alpha-FP in the alpha 1-globulin band. At the same gestational age, protein electrophoresis of amniotic fluid revealed four bands corresponding to albumin, alpha-FP, haptoglobin and transferrin. Creatinine levels were significantly lower (P less than 0.01) in amniotic fluid than in exocoelomic fluid, and alpha-FP levels were similar in both exocoelomic and amniotic fluids. These results suggest that the exocoelomic fluid is a transudate of the maternal serum except for the presence of high levels of alpha-FP, that amniotic and exocoelomic cavities are separated by a non-permeable membrane and that the secondary yolk sac plays an important role in early protein synthesis and transfer.
Gulbis
B
B
Department of Clinical Chemistry, Academic Hospital Erasme, Brussels, Belgium.
Jauniaux
E
E
Jurkovic
D
D
Thiry
P
P
Campbell
S
S
Ooms
H A
HA
D000418:Albumins / Q000378:metabolism; D000653:Amniotic Fluid / Q000378:metabolism; D001826:Body Fluids / Q000378:metabolism; D002712:Chlorides / Q000378:metabolism; D003404:Creatinine / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D004622:Embryo, Mammalian / Q000378:metabolism*; D005260:Female; D005865:Gestational Age; D006242:Haptoglobins / Q000378:metabolism; D006801:Humans; D015151:Immunoblotting; D011247:Pregnancy; D011506:Proteins / Q000378:metabolism*; D014168:Transferrin / Q000378:metabolism; D000509:alpha-Fetoproteins / Q000378:metabolism
D000418:Albumins; D002712:Chlorides; D006242:Haptoglobins; D011506:Proteins; D014168:Transferrin; D000509:alpha-Fetoproteins; D003404:Creatinine