Isolation and characterization of nuclei from rice embryos.
A method has been developed to isolate pure preparations of nuclei in high yield from commercially available viable rice embryos (germ), employing extraction with buffer solution containing glycerol (without detergent) and polyamine, followed by centrifugation on a 30% Percoll cushion. The intactness of the isolated nuclei was confirmed by light microscopy as well as electron microscopy. The protein profiles of both whole nuclei and nuclear extracts obtained by SDS-PAGE, organellar marker enzyme activities, DNA and RNA analyses, and in vitro RNA synthesis, all indicate that the highly purified nuclei are isolated from rice embryos.
Yamaguchi
J
J
Research Institute for Biochemical Regulation, School of Agricultural Sciences, Nagoya University, Japan.
Lim
P Y
PY
Aratani
K
K
Akazawa
T
T
D002458:Cell Fractionation; D002467:Cell Nucleus / Q000737:chemistry* / Q000648:ultrastructure; D004247:DNA / Q000032:analysis; D005786:Gene Expression Regulation; D008853:Microscopy; D009687:Nuclear Proteins / Q000032:analysis*; D012275:Oryza / Q000737:chemistry / Q000196:embryology / Q000235:genetics*; D010940:Plant Proteins / Q000032:analysis*; D012313:RNA / Q000032:analysis; D012639:Seeds / Q000737:chemistry* / Q000648:ultrastructure; D014158:Transcription, Genetic
D009687:Nuclear Proteins; D010940:Plant Proteins; D012313:RNA; D004247:DNA