GABA-like immunoreactive neurons in the retina of Bufo marinus: evidence for the presence of GABA-containing ganglion cells.
gamma-Aminobutyric acid (GABA)-like immunoreactive (IR) neurons in the retina of the cane toad Bufo marinus were revealed using immunohistochemistry on retinal wholemount preparation and sectioned material. GABA-IR neurons included horizontal, bipolar and amacrine cells in the inner nuclear layer and small to medium sized cells in the ganglion cell layer. A few IR axons were seen in the optic fiber layer of the retina. Following the injection of the carbocyanine dye, DiI into the optic tectum ganglion cells were retrogradely filled. A small population of DiI-filled ganglion cells (2.8%) was found to be GABA-IR. GABA-IR neurons in the ganglion cell layer without DiI label were considered to be displaced amacrine cells of which 45.3% were GABA positive. It is proposed that GABA-containing ganglion cells may form an inhibitory projection to visual centers of the anuran brain.
Gábriel
R
R
Department of Anatomy and Histology, Flinders University of South Australia, Adelaide.
Straznicky
C
C
Wye-Dvorak
J
J
D000818:Animals; D002024:Bufo marinus / Q000378:metabolism*; D002232:Carbocyanines; D016650:Fluorescein-5-isothiocyanate; D005456:Fluorescent Dyes; D007150:Immunohistochemistry; D009474:Neurons / Q000737:chemistry* / Q000378:metabolism; D012160:Retina / Q000737:chemistry* / Q000166:cytology / Q000378:metabolism; D012165:Retinal Ganglion Cells / Q000737:chemistry* / Q000378:metabolism; D013194:Staining and Labeling; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
C035005:3,3'-dihexadecylindocarbocyanine; D002232:Carbocyanines; D005456:Fluorescent Dyes; D005680:gamma-Aminobutyric Acid; D016650:Fluorescein-5-isothiocyanate