The use of nylon wool for the isolation of T lymphocyte subpopulations.
A simple method for the affinity isolation of lymphocyte subpopulations using nylon wool as a solid phase matrix is described. This matrix was coated with an acid-treated IgG fraction of polyclonal antibodies against mouse immunoglobulins. Using this approach, a simple isolation procedure for lymphocyte subpopulations, yielding purities higher than 95% was developed. The method can be used on a large scale for positive and negative cell selection, with very little non-specific binding. The isolated populations are fully functional.
Kokkinopoulos
D
D
Department of Immunology, Hellenic Anticancer Institute, Athens, Greece.
Perez
S
S
Sotiriadou
R
R
Stinios
J
J
Papamichail
M
M
D000818:Animals; D000911:Antibodies, Monoclonal; D002455:Cell Division; D002469:Cell Separation / Q000379:methods*; D002675:Child, Preschool; D002846:Chromatography, Affinity / Q000379:methods*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007074:Immunoglobulin G; D016130:Immunophenotyping; D007223:Infant; D009757:Nylons; D016176:T-Lymphocyte Subsets / Q000276:immunology / Q000502:physiology
D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D009757:Nylons