Alteration of RNA I metabolism in a temperature-sensitive Escherichia coli rnpA mutant strain.
E. coli strain A49 carries the themosensitive mutation in the rnpA gene encoding the protein component of RNase P, a tRNA-processing enzyme. Two small RNAs were highly accumulated in the A49 carrying derivatives of ColE1-type plasmids, at nonpermissive temperature. Characterization of these RNAs showed that they were the processed or degraded products derived from RNA I, which is the negative controller of ColE1-type plasmid replication. These derivatives of RNA I only differ in size at the 5' ends. The data of their degradation and synthesis kinetics suggest that they are intermediates of RNA I metabolism.
Jung
Y H
YH
Department of Chemistry, Korea Advanced Institute of Science and Technology, Taejon.
Park
I
I
Lee
Y
Y
D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D004722:Endoribonucleases / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D010957:Plasmids; D012329:RNA, Bacterial / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D016337:RNA, Catalytic / Q000235:genetics*; D043262:Ribonuclease P; D013696:Temperature
D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; C042027:RNA I; D012329:RNA, Bacterial; D016337:RNA, Catalytic; D004722:Endoribonucleases; D043262:Ribonuclease P; C470865:ribonuclease P, E coli