title: Colocalization of nitric oxide synthase and NADPH-diaphorase in rat adrenal gland.
abstract: The distribution and colocalization of nitric oxide synthase (NOS) and reduced nicotinamide adenine dinucleotide phosphate (NADPH)-diaphorase was studied in the neuronal elements of the adrenal gland of the rat. Ganglion cells and many nerve fibres in the gland showed both NOS-immunoreactivity and NADPH-diaphorase staining. The adrenal cortical cells showed NADPH-diaphorase staining but were not immunoreactive for NOS. Positive labelling for both NADPH-diaphorase and NOS was found in bundles and in single fibres with varicosities, preferentially located around the noradrenaline (NA)-storing cells. Adrenaline (A)-storing cells and ganglion cells in the medulla, along with the cortical cells and blood vessels in the zona glomerulosa, received relatively fewer positive fibres.
authors.affiliation: Department of Anatomy and Developmental Biology, University College, London, UK.
authors.forename: M
authors.initials: M
authors.lastname: Afework
authors.forename: A
authors.initials: A
authors.lastname: Tomlinson
authors.forename: A
authors.initials: A
authors.lastname: Belai
authors.forename: G
authors.initials: G
authors.lastname: Burnstock
mesh_terms: D000311:Adrenal Glands / Q000033:anatomy & histology / Q000201:enzymology* / Q000294:innervation; D000594:Amino Acid Oxidoreductases / Q000378:metabolism*; D000818:Animals; D004837:Epinephrine / Q000378:metabolism; D005724:Ganglia / Q000166:cytology / Q000378:metabolism; D006651:Histocytochemistry; D008297:Male; D009252:NADPH Dehydrogenase / Q000378:metabolism*; D009412:Nerve Fibers / Q000201:enzymology; D019001:Nitric Oxide Synthase; D009638:Norepinephrine / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
chemical_list: D019001:Nitric Oxide Synthase; D000594:Amino Acid Oxidoreductases; D009252:NADPH Dehydrogenase; D009638:Norepinephrine; D004837:Epinephrine