Similarity between mycobacterial and human epidermal antigens.
Eight out of 17 mouse anti-Mycobacterium leprae monoclonal antibodies (MAb) were previously observed to react with human nerve and skin antigenic determinants in cryostat sections, using an indirect immunoperoxidase technique. These observations suggested that antigenic mimicry may be involved in the development of the clinical manifestations of leprosy. In the present study we have extended our earlier findings by investigating sera from leprosy patients and MAb using Western blot technique. It was observed that 30 sera and their corresponding F(ab')2 fragments from isolated IgG fractions of both tuberculoid and lepromatous patients reacted with 40-50 epidermal proteins of molecular weights (MW) ranging from 10 to 130 kDa. Sera from 14 controls, however, showed similar reactivity patterns. Absorption of nine patient and control sera with M. tuberculosis, M. marinum and M. kansasii resulted in the removal of several components of different MW in nine, four and three cases, respectively. No consistent differences between sera from leprosy patients and controls were observed. Four out of eight MAb against M. leprae which reacted with determinants in human epidermis and/or dermis in skin cryostat sections reacted with epidermal proteins of MW higher than 39 kDa in Western blot. Four MAb which showed reactivity in cryostat sections did not react in Western blot. Another four MAb did react with human epidermal proteins in Western blot but did not react in cryostat sections, indicating that the MAb were reacting with different epitopes in the two systems. Five MAb did not react with human epidermal proteins either in cryostat sections or in Western blot.(ABSTRACT TRUNCATED AT 250 WORDS)
van den Akker
T W
TW
Department of Dermato-Venereology, Academic Hospital Dijkzigt, Rotterdam, The Netherlands.
Naafs
B
B
Kolk
A H
AH
De Glopper-van der Veer
E
E
Chin
R A
RA
Lien
A
A
Van Joost
T
T
D000818:Animals; D000911:Antibodies, Monoclonal; D015153:Blotting, Western; D016616:Cryoultramicrotomy; D004817:Epidermis / Q000276:immunology*; D000939:Epitopes / Q000276:immunology*; D005260:Female; D006801:Humans; D007108:Immune Tolerance; D007163:Immunosorbent Techniques; D007918:Leprosy / Q000276:immunology*; D051379:Mice / Q000276:immunology; D008970:Molecular Weight; D009161:Mycobacterium / Q000276:immunology*; D009166:Mycobacterium leprae / Q000276:immunology; D009169:Mycobacterium tuberculosis / Q000276:immunology; D009170:Nontuberculous Mycobacteria / Q000276:immunology
D000911:Antibodies, Monoclonal; D000939:Epitopes