Induction of karyological damage by bleomycin in polyploidizating rabbit hepatocytes.
The effects of the antitumor drug bleomycin on polyploidizating hepatocytes of immature rabbits were investigated by combining cytochemical (static microfluorometry) and morphological (fluorescence and electron microscopy) approaches. In addition to a decelerating effect of the drug on the cell kinetics, there were several types of karyological damage (micronuclei, internuclear chromatin bridges, mitotic chromosome bridges), thus demonstrating a clastogenic activity of bleomycin on the DNA, Moreover, anomalous nuclear and cytoplasmic divisions suggest interference of the drug with other cellular structures (mainly the microtubule network) responsible for the cell kinetics.
Barni
S
S
Dipartimento di Biologia Animale, Università di Pavia, Italy.
Porcelli
F
F
Scherini
E
E
Spadari
S
S
D000818:Animals; D001761:Bleomycin / Q000633:toxicity*; D002875:Chromosomes / Q000187:drug effects*; D004247:DNA / Q000032:analysis; D004249:DNA Damage; D005260:Female; D008099:Liver / Q000187:drug effects* / Q000648:ultrastructure; D048629:Micronuclei, Chromosome-Defective / Q000187:drug effects; D008938:Mitosis / Q000187:drug effects; D011123:Polyploidy; D011817:Rabbits
D001761:Bleomycin; D004247:DNA