Association of (3H) DNA with fowl spermatozoa and their in vitro fertilisation of hamster ova.
1. Fowl spermatozoa incubated with DNA were used for the fertilisation of zona-free hamster oocytes. 2. Two categories of spermatozoa were detected: those which were not labelled and those which showed a very high degree of labelling (19 +/- 2% of the whole population). 3. In the hamster oocytes exposed to treated spermatozoa the labelled sperm heads were identified.
Trefil
P
P
Research Institute of Animal Production, Uhrínĕves, Czechoslovakia.
Kopecný
V
V
Hájková
M
M
Petr
J
J
Míka
J
J
D000818:Animals; D001345:Autoradiography; D002645:Chickens; D006224:Cricetinae; D004247:DNA / Q000032:analysis; D005260:Female; D005307:Fertilization in Vitro; D008297:Male; D013084:Sperm-Ovum Interactions; D013094:Spermatozoa / Q000737:chemistry; D014162:Transfection / Q000379:methods*; D014316:Tritium
D014316:Tritium; D004247:DNA