[The interaction of the causative agent of melioidosis with the host's alveolar macrophages].
Studies were carried out on guinea pigs and albino rats, intranasally infected with P. pseudomallei C-141. The cells of bronchovesicular exudate were obtained from animals 1, 4 and 24 hours after infection. Electron microscopy was applied to study the process of interaction of the agent and alveolar macrophages. Bacteria were shown to form a capsule which permitted avoiding phagocytosis, when entering the host respiratory system. Microbes that failed to form a capsule were absorbed by macrophages and enclosed in a phagosome. Then some bacteria were destroyed by the lysosomal enzymes, the other synthesized a capsule, which protected them against the effect of phagolysosome content. There were also such microbes which escaped from a phagosome prior to fusion with lysosomes and parasitized in phagocytic cytoplasma forming a capsule there. By the end of the first 24 hours of observation the intact encapsulated microbe species were found to prevail in the host cells.
Popov
S F
SF
Kurilov
V Ia
VIa
Lagutin
M P
MP
D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000382:microbiology; D016957:Burkholderia pseudomallei / Q000472:pathogenicity* / Q000648:ultrastructure; D006168:Guinea Pigs; D008171:Lung Diseases / Q000276:immunology / Q000382:microbiology*; D016676:Macrophages, Alveolar / Q000382:microbiology* / Q000648:ultrastructure; D008554:Melioidosis / Q000276:immunology / Q000382:microbiology*; D008854:Microscopy, Electron; D010587:Phagocytosis; D051381:Rats; D013997:Time Factors