Preparation and characterization of an antibody specific to the rat dopamine D2 receptor.
Antibodies specific to the dopamine D2 receptor have been raised in rabbits using synthetic peptides. The resulting antiserum was sensitive to picogram quantities of peptide as measured by enzyme-linked immunoassay and was shown to have a 33% cross-reactivity with partially purified D2 receptor protein. No detectable cross-reactivity with similarly prepared fungal membranes was observed. D2 receptor preparations from normal rat pituitary cells were used in Western blot analysis. Bands of M(r) = 95,000 and 34,000 were detected in these preparations with a third faint band at 120,000. These correspond to the pituitary D2 receptor.
Johnston
J M
JM
Unit of Metabolic Medicine, St Mary's Hospital Medical School, Imperial College of Science, Technology and Medicine, Paddington, London, U.K.
Wood
D F
DF
James
J D
JD
Johnston
D G
DG
D000818:Animals; D000906:Antibodies / Q000737:chemistry*; D000918:Antibody Specificity; D015153:Blotting, Western; D002462:Cell Membrane / Q000378:metabolism; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D010902:Pituitary Gland / Q000378:metabolism; D011817:Rabbits; D051381:Rats; D017448:Receptors, Dopamine D2 / Q000276:immunology* / Q000378:metabolism; D012232:Rhizoctonia
D000906:Antibodies; D017448:Receptors, Dopamine D2