Different modifications by vanillin in cytotoxicity and genetic changes induced by EMS and H2O2 in cultured Chinese hamster cells.
The modifying effects of vanillin on the cytotoxicity and 6-thioguanine (6TG)-resistant mutations induced by two different types of chemical mutagens, ethyl methanesulfonate (EMS) and hydrogen peroxide (H2O2), were examined using cultured Chinese hamster V79 cells. The effects of vanillin on H2O2-induced chromosome aberrations were also examined. Vanillin had a dose-dependent enhancing effect on EMS-induced cytotoxicity and 6TG-resistant mutations, when cells were simultaneously treated with vanillin. The post-treatment with vanillin during the mutation expression time of cells after treatment with EMS also showed an enhancement of the frequency of mutations induced by EMS. However, vanillin suppressed the cytotoxicity induced by H2O2 when cells were post-treated with vanillin after H2O2 treatment. Vanillin showed no change in the absence of activity of H2O2 to induce mutations. Post-treatment with vanillin also suppressed the chromosome aberrations induced by H2O2. The differential effects of vanillin were probably due to the quality of mutagen-induced DNA lesions and vanillin might influence at least two different kinds of cellular repair functions. The mechanisms by which vanillin enhances or suppresses chemical-induced cytotoxicity, mutations and chromosome aberrations are discussed.
Tamai
K
K
National Institute of Genetics, Shizuoka, Japan.
Tezuka
H
H
Kuroda
Y
Y
D000818:Animals; D001547:Benzaldehydes / Q000494:pharmacology*; D002478:Cells, Cultured; D016009:Chi-Square Distribution; D002869:Chromosome Aberrations / Q000235:genetics; D006224:Cricetinae; D003412:Cricetulus; D004260:DNA Repair / Q000187:drug effects; D005020:Ethyl Methanesulfonate / Q000633:toxicity*; D005421:Flavoring Agents / Q000494:pharmacology*; D006861:Hydrogen Peroxide / Q000633:toxicity*; D008297:Male; D016296:Mutagenesis / Q000187:drug effects*; D009152:Mutagenicity Tests; D009154:Mutation / Q000187:drug effects; D013866:Thioguanine
D001547:Benzaldehydes; D005421:Flavoring Agents; D005020:Ethyl Methanesulfonate; D006861:Hydrogen Peroxide; C100058:vanillin; D013866:Thioguanine