Exploiting the chemical synthesis of RNA.
A number of nucleotide phosphoramidites are now available that permit the chemical synthesis of RNA, modified RNA and RNA-DNA chimeric oligonucleotides. Since the chemical strategy allows the introduction of a particular modification at any given site in a nucleotide polymer, very subtle and specific questions regarding structure-function relationships in RNA may be addressed.
Usman
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Department of Biology, Massachusetts Institute of Technology, Cambridge 02139.
Cedergren
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D001483:Base Sequence; D002678:Chimera; D004247:DNA / Q000737:chemistry; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D012313:RNA / Q000138:chemical synthesis* / Q000737:chemistry; D013329:Structure-Activity Relationship
D012313:RNA; D004247:DNA