title: Typing of human red cell and serum mixtures in three electrophoretic systems.
abstract: The isoenzymes erythrocyte acid phosphatase and phosphoglucomutase were typed in mixed red cell samples which had been derived from two individuals; the protein group specific component was typed in mixed serum samples. Typing was performed by isoelectric focusing on ultrathin polyacrylamide gels. Depending upon the mixture, from 2 to 20% (but typically 5-10%) by volume of a second blood or serum needed to be present in a mixture before it could be detected. In the majority of cases when there was significant mixing, samples were readily identified as a mixture when the results consisted of unusual band patterns or unusual band intensities. There would be a few instances when blood or serum could not be identified as a mixture when masking effects occurred or when the mixture produced a combined, apparently normal, pattern.
authors.affiliation: Criminalistics and Forensic Biology Section, Department of Scientific and Industrial Research, Petone, Wellington, New Zealand.
authors.forename: P
authors.initials: P
authors.lastname: Stringer
authors.forename: S K
authors.initials: SK
authors.lastname: Vintiner
authors.forename: L I
authors.initials: LI
authors.lastname: Stowell
authors.forename: D G
authors.initials: DG
authors.lastname: Thomson
mesh_terms: D000135:Acid Phosphatase / Q000097:blood*; D001788:Blood Grouping and Crossmatching / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D004912:Erythrocytes / Q000201:enzymology; D006801:Humans; D007527:Isoenzymes / Q000097:blood*; D010733:Phosphoglucomutase / Q000097:blood*; D014809:Vitamin D-Binding Protein / Q000097:blood*
chemical_list: D007527:Isoenzymes; D014809:Vitamin D-Binding Protein; D000135:Acid Phosphatase; D010733:Phosphoglucomutase