X-ray induction of micronuclei in human lymphocyte subpopulations differentiated by immunoperoxidase staining.
In this study we sought to confirm the radiosensitivity of human peripheral blood lymphocyte subpopulations using a micronucleus assay. Mononucleated cells isolated from peripheral blood were irradiated with X rays. After being cultured for 3 days, cells were fixed and stained using the immunoperoxidase staining technique. Lymphocyte subpopulations were characterized by means of the monoclonal antibodies Leu4 (CD3), Leu2a (CD8) and Leu19 (CD56). Dose-response curves were obtained by scoring the number of micronuclei in binucleated cells that reacted with a specific antibody and were then stained. The dose response of CD8+ (suppressor/cytotoxic) cells was quite similar to that of CD3+ (pan T) cells. In comparison, CD56+ (natural killer) cells were significantly less sensitive, although scorable binucleated CD56+ cells made up less than 4% of the total number of binucleated cells.
Ban
S
S
Department of Genetics, Radiation Effects Research Foundation, Hiroshima, Japan.
Cologne
J B
JB
D000328:Adult; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D066298:In Vitro Techniques; D016131:Lymphocyte Subsets / Q000502:physiology / Q000528:radiation effects*; D008297:Male; D015162:Micronucleus Tests; D011836:Radiation Tolerance / Q000502:physiology*; D013194:Staining and Labeling