Characterization of antibodies against human N-terminal parathyroid hormone by epitope mapping.
Two polyclonal antisera from goat and mouse and two monoclonal antibodies against human parathyroid hormone (1-34) were characterised by epitope mapping. Hexapeptides were synthesized on polystyrene pins, the sequences of which overlapped and represented the entire sequence of hPTH(1-34). Binding of antibodies to these hexapeptides was determined and antigenic determinants thus characterized. At least one predominant binding sequence was detected in the region of hPTH(7-14).
Tampe
J
J
Immundiagnostik, Bensheim, Germany.
Broszio
P
P
Manneck
H E
HE
Missbichler
A
A
Blind
E
E
Müller
K B
KB
Schmidt-Gayk
H
H
Armbruster
F P
FP
D000595:Amino Acid Sequence; D000906:Antibodies; D000911:Antibodies, Monoclonal; D000939:Epitopes / Q000737:chemistry; D006801:Humans; D007120:Immunochemistry; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010281:Parathyroid Hormone / Q000737:chemistry / Q000276:immunology*; D010446:Peptide Fragments / Q000737:chemistry / Q000276:immunology*; D010449:Peptide Mapping; D011487:Protein Conformation; D019379:Teriparatide
D000906:Antibodies; D000911:Antibodies, Monoclonal; D000939:Epitopes; D010281:Parathyroid Hormone; D010446:Peptide Fragments; D019379:Teriparatide