Isoelectric focusing of human hair keratins: correlation with sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) patterns and effect of cosmetic treatments.
A new isoelectric focusing (IEF) technique in polyacrylamide gels with 6M urea and 1.5% Nonidet P40 has been developed to characterize human hair samples. The phenotypes demonstrated with this procedure has been correlated with the sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) patterns described by other authors. The method described can be applied in the forensic science analysis of a single human hair. Using the same IEF technique we have studied the changes in electrophoretic patterns of cosmetically treated hair. The characteristics of the modifications observed and its utility in forensic science work are also discussed in this paper.
Rodriguez-Calvo
M S
MS
Department of Legal Medicine, University of Santiago de Compostela, Galicia, Spain.
Carracedo
A
A
Muñoz
I
I
Concheiro
L
L
D003902:Detergents; D004591:Electrophoresis, Polyacrylamide Gel; D006197:Hair / Q000737:chemistry* / Q000187:drug effects; D006203:Hair Preparations / Q000494:pharmacology*; D006801:Humans; D007525:Isoelectric Focusing; D007633:Keratins / Q000737:chemistry* / Q000187:drug effects; D017830:Octoxynol; D010641:Phenotype; D011092:Polyethylene Glycols; D015203:Reproducibility of Results; D012995:Solubility; D014508:Urea
D003902:Detergents; D006203:Hair Preparations; D011092:Polyethylene Glycols; D007633:Keratins; D014508:Urea; D017830:Octoxynol; C010615:Nonidet P-40