Immunoelectron microscopic localization of microsomal alanine aminopeptidase.
The localization of microsomal alanine aminopeptidase was investigated in the rat kidney. Resin embedding failed to demonstrate the localization of the enzyme by immunogold labelling. Using a cryo-ultramicrotomy method the enzyme could be detected on the luminal side of the brush border membrane of proximal tubular cells and to a lesser degree in their mitochondria. Furthermore, vesicular structures labelled with gold were found in the cytoplasma in the apical region of these cells.
Kettmann
U
U
Institute of Biochemistry, Faculty of Medicine, University of Halle, Germany.
Humbel
B
B
Holzhausen
H J
HJ
Bahn
H
H
Aurich
H
H
D000626:Aminopeptidases / Q000032:analysis*; D000818:Animals; D018826:CD13 Antigens; D007150:Immunohistochemistry; D007668:Kidney / Q000201:enzymology*; D007687:Kidney Tubules, Proximal / Q000201:enzymology* / Q000648:ultrastructure; D016253:Microscopy, Immunoelectron; D008861:Microsomes / Q000201:enzymology; D008871:Microvilli / Q000201:enzymology; D008928:Mitochondria / Q000201:enzymology; D051381:Rats; D013194:Staining and Labeling
D000626:Aminopeptidases; D018826:CD13 Antigens