Modulation by novobiocin of sister-chromatid exchanges induced by tumor necrosis factor in human lymphocytes.
The effect of the antibiotic novobiocin on human recombinant tumor necrosis factor (TNF)-induced sister-chromatid exchanges (SCEs) were examined in human peripheral blood lymphocytes. TNF, when introduced in a dose range of 10-1000 U/ml at the initiation of culture, was found to cause a significant increase in SCE frequency. The simultaneous addition of TNF and novobiocin (25 micrograms/ml) in the assay resulted in no increase of SCE frequency. Delayed (for 24 h) addition of novobiocin suppressed the induction of SCEs by 50, 100 and 500 U/ml but not by 1000 U/ml of TNF.
Lazutka
J R
JR
Ecological Genetics Laboratory, Vilnius University, Lithuania.
Rudaitiene
S
S
D000328:Adult; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects; D009675:Novobiocin / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000494:pharmacology; D012854:Sister Chromatid Exchange / Q000187:drug effects*; D059005:Topoisomerase II Inhibitors; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors*
D011994:Recombinant Proteins; D059005:Topoisomerase II Inhibitors; D014409:Tumor Necrosis Factor-alpha; D009675:Novobiocin