Inhibition of proliferation and differentiation of human B-lymphocytes by a biscoclaurine alkaloid.
A biscoclaurine alkaloid, cepharanthine, is known to be a potent inhibitor of snake venom-induced hemolysis by interaction with the lipid bilayer of the membrane. The drug also interferes with the ion channel intracellularly. In this study, we examined the effect of cepharanthine on human B-cell functions. Small dense B-cells from tonsil samples were isolated using a Percoll density gradient from non-rosetted cells and were used as the target cells. Cepharanthine inhibited the proliferation of the lymphocytes and antibody production of human B-lymphocytes. The inhibitory effect of cepharanthine on the proliferation was caused by the arrest of the late G1 to S phase transition in the cell cycle. However, the mechanism of suppression of antibody formation remains unknown. These results suggest that cepharanthine acts on human B-cells as an immunomodulating agent.
Morikawa
K
K
Department of Internal Medicine, Shimane Medical University, Japan.
Oseko
F
F
Morikawa
S
S
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000470:Alkaloids / Q000494:pharmacology*; D000944:Antigens, Differentiation, B-Lymphocyte / Q000032:analysis; D001402:B-Lymphocytes / Q000187:drug effects* / Q000502:physiology; D044182:Benzylisoquinolines; D002453:Cell Cycle / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D006801:Humans; D007136:Immunoglobulins / Q000096:biosynthesis; D008213:Lymphocyte Activation / Q000187:drug effects*; D011961:Receptors, Fc / Q000032:analysis; D017455:Receptors, IgE; D015375:Receptors, Interleukin-2 / Q000032:analysis; D052117:Benzodioxoles
D000276:Adjuvants, Immunologic; D000470:Alkaloids; D000944:Antigens, Differentiation, B-Lymphocyte; D044182:Benzylisoquinolines; D007136:Immunoglobulins; D011961:Receptors, Fc; D017455:Receptors, IgE; D015375:Receptors, Interleukin-2; C006947:cepharanthine; D052117:Benzodioxoles