title: Purification of recombinant human interferon-beta by immobilized antisense peptides.
abstract: Synthetic antisense peptides encoded in the antisense strands of DNA corresponding to the 1-14, 42-54 and 103-115 fragments of the human interferon-beta sequence were applied in the purification of recombinant human interferon-beta from a mammalian cell culture. The protein fragments were selected on the basis of their computer-predicted exposure on the surface of the protein. The antisense peptides were synthetized by the solid-phase method directly on the resin used as the stationary phase in affinity chromatography. All the tested antisense peptides showed a selective affinity for human interferon-beta, permitting a ten-fold purification of the protein.
authors.affiliation: SCLAVO SpA, Siena, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Scapol
authors.forename: P
authors.initials: P
authors.lastname: Rappuoli
authors.forename: G C
authors.initials: GC
authors.lastname: Viscomi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D016375:Antisense Elements (Genetics); D016466:CHO Cells; D002478:Cells, Cultured; D002846:Chromatography, Affinity / Q000379:methods; D002851:Chromatography, High Pressure Liquid; D006224:Cricetinae; D006801:Humans; D016899:Interferon-beta / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry* / Q000235:genetics; D011994:Recombinant Proteins / Q000302:isolation & purification; D013056:Spectrophotometry, Ultraviolet
chemical_list: D016375:Antisense Elements (Genetics); D010455:Peptides; D011994:Recombinant Proteins; D016899:Interferon-beta