title: Comparative analysis of 23S ribosomal RNA gene sequences of Bacillus anthracis and emetic Bacillus cereus determined by PCR-direct sequencing.
abstract: The primary structures of the 23S ribosomal RNA genes of Bacillus anthracis and an emetic strain of Bacillus cereus were determined by direct sequencing of enzymatically amplified chromosomal DNA. The 23S rRNA gene sequences of B. anthracis and B. cereus were found to be almost identical and showed only two differences (a single nucleotide change, and a single base insertion in B. cereus). The feasibility of using PCR-direct sequencing for the rapid sequence determination of large-subunit rRNA genes is demonstrated.
authors.affiliation: Department of Microbiology, AFRC Institute of Food Research, Reading Laboratory, UK.
authors.forename: C
authors.initials: C
authors.lastname: Ash
authors.forename: M D
authors.initials: MD
authors.lastname: Collins
mesh_terms: D001408:Bacillus anthracis / Q000737:chemistry / Q000235:genetics*; D001409:Bacillus cereus / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012329:RNA, Bacterial / Q000737:chemistry* / Q000235:genetics; D012338:RNA, Ribosomal, 23S / Q000737:chemistry* / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid
chemical_list: D012329:RNA, Bacterial; D012338:RNA, Ribosomal, 23S