[Sensitizing capacity, cross-reactivity and antigenic determinants of bisphenol A].
Bis-GMA and epoxy resins are known to cause allergic reactions in some patients. The purpose of this research was to investigate the sensitizing capacity, cross-reactivity and antigenic determinants of bisphenol A (BPA), a major constituent of Bis-GMA and BPA type epoxy resins. Anti-BPA antibodies were prepared from mice injected with BPA-ovalbumin conjugates (BPA-OVA), and the reactions of the structurally BPA-related compounds were compared with that of BPA using these antibodies by the enzyme immunoassay (EIA) and EIA inhibition test. The findings are as follows: 1) Injection of BPA failed to generate anti-BPA antibodies. However, injection of BPA-OVA was effective in producing antibodies strongly reacting with BPA. 2) Polyclonal anti-BPA antibodies cross-reacted with bisphenol B (BPB), p-isopropyl-phenol (IPP), diethylstilbestrol (DES) and 2,4-dinitrophenyl (DNP). 3) Five kinds of monoclonal anti-BPA antibodies were obtained. These monoclonal antibodies reacted specifically with BPA and moderately cross-reacted with IPP, DES and BPB, but not with DNP. The results suggest that BPA-OVA is capable of eliciting anti-BPA antibody production in mice and that the antigenic determinant of BPA is the structure of one central carbon atom, covalently bound with two phenyl groups and two methyl groups.
Orihara
J
J
Department of Biomaterials Science, Faculty of Dentistry, Tokyo Medical and Dental University.
D000818:Animals; D000906:Antibodies / Q000032:analysis / Q000276:immunology; D000917:Antibody Formation; D001559:Benzhydryl Compounds; D017438:Bisphenol A-Glycidyl Methacrylate; D003429:Cross Reactions; D000939:Epitopes; D005260:Female; D007114:Immunization; D051379:Mice; D010636:Phenols / Q000276:immunology*; D011109:Polymethacrylic Acids; D012387:Root Canal Filling Materials; D000098826:Bisphenol A Compounds
D000906:Antibodies; D001559:Benzhydryl Compounds; D017438:Bisphenol A-Glycidyl Methacrylate; D000939:Epitopes; D010636:Phenols; D011109:Polymethacrylic Acids; D012387:Root Canal Filling Materials; C006780:bisphenol A; D000098826:Bisphenol A Compounds