title: The determination of asialoglycoforms of serum glycoproteins by lectin blotting with Ricinus communis agglutinin.
abstract: Serum proteins were fractionated by polyacrylamide gel electrophoresis under denaturing conditions and transferred to nitrocellulose membranes. The blotted polypeptides were probed with biotinylated Ricinus communis lectin (RCA120) followed by streptavidin/alkaline phosphatase. This procedure detected five asialoglycoproteins (alpha 2-macroglobulin, transferrin, alpha 1-antitrypsin, alpha 1-antichymotrypsin and haptoglobin beta chain). The asialoform of the alpha 1-trypsin inhibitor was found to be decreased in inflammation.
authors.affiliation: Division of Biochemistry, Queen's University, Belfast, Northern Ireland, UK.
authors.forename: F T
authors.initials: FT
authors.lastname: Lundy
authors.forename: G B
authors.initials: GB
authors.lastname: Wisdom
mesh_terms: D000469:Alkaline Phosphatase; D001212:Asialoglycoproteins / Q000097:blood*; D001426:Bacterial Proteins; D001710:Biotin; D003101:Collodion; D004591:Electrophoresis, Polyacrylamide Gel; D006242:Haptoglobins / Q000032:analysis; D006801:Humans; D015151:Immunoblotting; D007249:Inflammation / Q000097:blood; D037102:Lectins; D037121:Plant Lectins; D019809:Streptavidin; D014168:Transferrin / Q000032:analysis; D000514:alpha 1-Antichymotrypsin / Q000032:analysis; D000515:alpha 1-Antitrypsin / Q000032:analysis; D000511:alpha-Macroglobulins / Q000032:analysis
chemical_list: D001212:Asialoglycoproteins; D001426:Bacterial Proteins; D006242:Haptoglobins; D037102:Lectins; D037121:Plant Lectins; C017707:Ricinus communis agglutinin-1; D014168:Transferrin; D000514:alpha 1-Antichymotrypsin; D000515:alpha 1-Antitrypsin; D000511:alpha-Macroglobulins; D001710:Biotin; D003101:Collodion; D019809:Streptavidin; D000469:Alkaline Phosphatase