Enzymatic and immunological detection of G protein alpha-subunits in the pathogenic fungus Candida albicans.
GTP stimulation of adenylyl cyclase from the dimorphic pathogenic fungus Candida albicans is greatly enhanced by preincubation of membrane proteins with cholera toxin, NAD and ATP. In the presence of [32P]NAD the toxin catalyzes the covalent incorporation of radioactivity into a membrane protein of 40 kDa. Pertussis toxin catalyzes the transference of the radioactivity from [32P]NAD to a 32 kDa protein. Two major proteins of 40-42 and 30-32 kDa can also be recognized in Western blots by an anti G alpha-common antibody. The results support the idea that G proteins are part of the hormone sensory transduction chain of Candida [(1990) Biochem. Biophys. Res. Commun. 167, 1177-1183].
Paveto
C
C
Departamento de Quimica Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina.
Montero
L
L
Passeron
S
S
D000255:Adenosine Triphosphate / Q000378:metabolism; D037361:Adenylate Cyclase Toxin; D000262:Adenylyl Cyclases / Q000187:drug effects / Q000378:metabolism; D015153:Blotting, Western; D002176:Candida albicans / Q000737:chemistry* / Q000201:enzymology; D002772:Cholera Toxin / Q000494:pharmacology; D019204:GTP-Binding Proteins / Q000032:analysis* / Q000276:immunology; D006160:Guanosine Triphosphate / Q000378:metabolism; D008565:Membrane Proteins / Q000187:drug effects / Q000378:metabolism; D009243:NAD / Q000378:metabolism; D037342:Pertussis Toxin; D011065:Poly(ADP-ribose) Polymerases / Q000187:drug effects / Q000378:metabolism; D010566:Virulence Factors, Bordetella / Q000494:pharmacology
D037361:Adenylate Cyclase Toxin; D008565:Membrane Proteins; D010566:Virulence Factors, Bordetella; D009243:NAD; D006160:Guanosine Triphosphate; D000255:Adenosine Triphosphate; D002772:Cholera Toxin; D011065:Poly(ADP-ribose) Polymerases; D037342:Pertussis Toxin; D019204:GTP-Binding Proteins; D000262:Adenylyl Cyclases