title: Tumor cell surface-associated binding site for the M(r) 72,000 type IV collagenase.
abstract: We have studied the capacity of two human breast adenocarcinoma cells, MDA-MB231 and MCF-7, to bind exogenous M(r) 72,000 type IV collagenase by both morphological and radioreceptor binding assays. By indirect immunofluorescence, staining with a specific anti-M(r) 72,000 type IV collagenase antibody was strongly induced when cells were preincubated with the purified enzyme. Scatchard plot analysis indicated the existence of a binding site for the M(r) 72,000 type IV collagenase with high affinity for both cell lines (Kd = 2 x 10(-9) M). These results are the first demonstration of the existence of a tumor cell membrane-associated putative receptor for a member of the matrix metalloproteinase family, as previously evidenced for the urokinase-type plasminogen activator.
authors.affiliation: Laboratory of Biology, University of Liege, Belgium.
authors.forename: H P
authors.initials: HP
authors.lastname: Emonard
authors.forename: A G
authors.initials: AG
authors.lastname: Remacle
authors.forename: A C
authors.initials: AC
authors.lastname: Noël
authors.forename: J A
authors.initials: JA
authors.lastname: Grimaud
authors.forename: W G
authors.initials: WG
authors.lastname: Stetler-Stevenson
authors.forename: J M
authors.initials: JM
authors.lastname: Foidart
mesh_terms: D000230:Adenocarcinoma / Q000201:enzymology*; D000595:Amino Acid Sequence; D001666:Binding Sites, Antibody; D001943:Breast Neoplasms / Q000201:enzymology*; D017364:Collagenases / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D006801:Humans; D007700:Kinetics; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011869:Radioligand Assay; D014407:Tumor Cells, Cultured
chemical_list: D017364:Collagenases