title: Enhancement of murine macrophage binding of and response to bacterial lipopolysaccharide (LPS) by LPS-binding protein.
abstract: We have studied the effects of highly purified rabbit lipopolysaccharide (LPS)-binding protein (LBP) on the ability of murine bone marrow-derived macrophages to respond to bacterial LPS. Macrophage responses studied include the secretion of tumor necrosis factor alpha, production of arginine-derived nitrite (NO2-), and killing of an intracellular pathogen, Leishmania enriettii. Macrophages from either CBA or LPS-hyporesponsive C3H/HeJ mice exhibited significantly greater sensitivity to LPS in the presence of LBP. Furthermore, both CBA and C3H/HeJ macrophages demonstrated an LBP-dependent enhancement of LPS binding. These results suggest that C3H/HeJ macrophages are capable of binding LPS-LBP complexes and support the hypothesis that hyporesponsiveness in this strain involves a step subsequent to LPS binding. Furthermore, these findings provide additional evidence of the important role played by the acute-phase plasma protein LBP in modifying host response to LPS.
authors.affiliation: Institute of Biochemistry, University of Lausanne, Epalinges, Switzerland.
authors.forename: S B
authors.initials: SB
authors.lastname: Corradin
authors.forename: J
authors.initials: J
authors.lastname: Mauël
authors.forename: P
authors.initials: P
authors.lastname: Gallay
authors.forename: D
authors.initials: D
authors.lastname: Heumann
authors.forename: R J
authors.initials: RJ
authors.lastname: Ulevitch
authors.forename: P S
authors.initials: PS
authors.lastname: Tobias
mesh_terms: D000209:Acute-Phase Proteins; D000818:Animals; D002352:Carrier Proteins / Q000494:pharmacology*; D002469:Cell Separation / Q000379:methods; D002478:Cells, Cultured; D005434:Flow Cytometry; D007891:Leishmania / Q000187:drug effects; D008070:Lipopolysaccharides / Q000378:metabolism; D008262:Macrophage Activation / Q000187:drug effects; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D008562:Membrane Glycoproteins; D051379:Mice; D008809:Mice, Inbred C3H; D008808:Mice, Inbred CBA; D009573:Nitrites / Q000378:metabolism; D009585:Nitrogen Dioxide / Q000378:metabolism; D012680:Sensitivity and Specificity; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D000099231:Lipopolysaccharide-Binding Protein
chemical_list: D000209:Acute-Phase Proteins; D002352:Carrier Proteins; D008070:Lipopolysaccharides; D008562:Membrane Glycoproteins; D009573:Nitrites; D009585:Nitrogen Dioxide; D014409:Tumor Necrosis Factor-alpha; D000099231:Lipopolysaccharide-Binding Protein