title: The effects of different hexachlorocyclohexanes and cyclodienes on glucose uptake and inositol phospholipid synthesis in rat brain cortex.
abstract: The inositol lipids from rat brain miniprisms were deacylated and separated by anion-exchange chromatography in order to determine whether or not gamma-hexachlorocyclohexane (gamma-HCH, lindane) and related compounds affect the different phosphatidylinositols. The incorporation of myo-[2-3H]inositol into phosphatidylinositol, phosphatidylinositol monophosphate and phosphatidylinositol bisphosphate were inhibited by lindane and its delta-HCH isomer. The inhibitory effects on phosphatidylinositol synthesis are not prominent in alpha-HCH and they are not significant with the beta-HCH and cyclodienes. The results presented here indicate that the inhibitory effect of lindane and delta-HCH on the phosphatidylinositol metabolism was no exclusively due to an interference with glucose transport. Lindane-treated miniprisms showed decreased myo-[2-3H]inositol uptake and, proportionately, an even greater inhibition of inositol phospholipid synthesis. Cellular uptake can, therefore, not account for all of the lindane inhibition.
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Universidad de Alcalá, Madrid, Spain.
authors.forename: J A
authors.initials: JA
authors.lastname: Pulido
authors.forename: N
authors.initials: N
authors.lastname: del Hoyo
authors.forename: M A
authors.initials: MA
authors.lastname: Pérez-Albarsanz
mesh_terms: D000818:Animals; D002540:Cerebral Cortex / Q000378:metabolism*; D002852:Chromatography, Ion Exchange; D005947:Glucose / Q000378:metabolism*; D001556:Hexachlorocyclohexane / Q000378:metabolism / Q000494:pharmacology*; D007294:Inositol / Q000378:metabolism; D008297:Male; D010716:Phosphatidylinositols / Q000096:biosynthesis* / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D010716:Phosphatidylinositols; D007294:Inositol; D001556:Hexachlorocyclohexane; D005947:Glucose