The effect of the anoxic radiosensitizing agent TAN on induction of revertants by gamma-rays and helium ions in Salmonella tester strains.
The modification effect of the anoxic radiosensitizer TAN on the mutagenesis in various Salmonella tester strains after gamma-ray and helium ion irradiation was studied. The oxygen enhancement ratios (OER) for all 3 strains on the lethal assay after gamma-irradiation are approximately equal to 2. The induction of reversions in TA98 and TA100 does not modify under anoxia. The value of OER on the mutagenic assay in TA102 equals 1.6. The OER after helium ion irradiation on the lethal and mutagenic assays was less than after gamma-irradiation. The mutagenesis in 3 strains after irradiation under anoxia is enhanced by TAN. The value of the TAN modification effect after gamma-irradiation increases from 2.1 +/- 0.2 for TA102 to 5.2 +/- 0.4 for TA100. However, the TAN influence on mutagenesis in TA100 after helium ion irradiation decreases to 3.1 +/- 0.3. We conclude that peculiarities of mutagenesis in various tester strains under anoxia with TAN can be explained by considering the nature of premutational DNA damages.
Basha
S G
SG
Joint Institute for Nuclear Research, Moscow Region, U.S.S.R.
Krasavin
E A
EA
Kozubek
S
S
D000693:Anaerobiosis; D004249:DNA Damage; D004307:Dose-Response Relationship, Radiation; D004735:Energy Transfer; D005720:Gamma Rays; D006371:Helium; D016296:Mutagenesis; D009152:Mutagenicity Tests; D013014:SOS Response, Genetics; D012486:Salmonella typhimurium / Q000187:drug effects / Q000528:radiation effects; D014216:Triacetoneamine-N-Oxyl / Q000494:pharmacology*
D006371:Helium; D014216:Triacetoneamine-N-Oxyl