Direct serum injection in ion chromatography on packing materials with a semi-permeable surface.
Reversed-phase packing materials with restricted access of proteins to the hydrophobic sites were tested for their applicability in the ion-interaction chromatography of anions. Especially C8-modified silica, which had been coated with a hydrophilic polymer acting as a semi-permeable barrier, could be used successfully for the separation of several anions in a proteinaceous matrix without removing the proteins prior to injection. In combination with UV or conductivity detection, this technique allows the determination of some physiologically important anions in serum samples.
Buchberger
W
W
Department of Analytical Chemistry, Johannes-Kepler-University, Linz, Austria.
Malissa
H
H
Mülleder
E
E
D000838:Anions; D001769:Blood; D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods*; D004563:Electrochemistry; D007477:Ions; D013056:Spectrophotometry, Ultraviolet; D013431:Sulfates / Q000032:analysis
D000838:Anions; D007477:Ions; D013431:Sulfates