title: RNA editing in the mitochondria of a conifer.
abstract: A 712-base portion of the mitochondrial gene coxI and the corresponding portion of the coxI transcript were amplified by PCR and by RT-PCR, respectively, from the gymnosperm western red cedar. Sequence comparison of amplified coxI DNA and mRNA revealed 26 C-to-U differences that are best explained by RNA editing of the type known to occur in angiosperms. This finding suggests that mitochondrial RNA-editing of the C-to-U type arose before the divergence of gymnosperms and angiosperms and can be considered a feature common to all higher plants.
authors.affiliation: Biotechnology Laboratory, University of British Columbia, Vancouver, Canada.
authors.forename: J C
authors.initials: JC
authors.lastname: Glaubitz
authors.forename: J E
authors.initials: JE
authors.lastname: Carlson
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D004272:DNA, Mitochondrial / Q000235:genetics*; D003576:Electron Transport Complex IV / Q000235:genetics*; D017343:Genes, Plant; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012313:RNA / Q000235:genetics*; D017393:RNA Editing; D014197:Trees / Q000235:genetics*
chemical_list: D004272:DNA, Mitochondrial; D010940:Plant Proteins; D012313:RNA; D003576:Electron Transport Complex IV