title: Increased resistance to the toxic effects of alkylating agents in tobacco expressing the E. coli DNA repair gene ada.
abstract: The protein coding region of the E. coli gene ada has been transferred to tobacco plants by a leaf disc transformation procedure involving an Agrobacterium tumefaciens Ti plasmid. Transformed plants were shown to be transgenic for the ada message and had increased levels of O6-alkylguanine DNA alkyltransferase activity. The N-methyl-N-nitrosourea- or taurinechlorethylnitrosourea-induced inhibition of growth of calluses or of cells in suspension was considerably lower in ada-transformed than in non-transformed plants. This indicates that O6-alkylguanine, O4-alkylthymine or phosphotriesters are growth-inhibitory lesions in tobacco.
authors.affiliation: Institute of Experimental Botany CSAV, Prague.
authors.forename: K
authors.initials: K
authors.lastname: Angelis
authors.forename: J
authors.initials: J
authors.lastname: Bríza
authors.forename: J
authors.initials: J
authors.lastname: Satava
authors.forename: I
authors.initials: I
authors.lastname: Skákal
authors.forename: J
authors.initials: J
authors.lastname: Velemínský
authors.forename: J
authors.initials: J
authors.lastname: Vlasák
authors.forename: K
authors.initials: K
authors.lastname: Kleibl
authors.forename: G P
authors.initials: GP
authors.lastname: Margison
mesh_terms: D016960:Agrobacterium tumefaciens / Q000235:genetics; D000477:Alkylating Agents / Q000633:toxicity*; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004260:DNA Repair / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial / Q000235:genetics; D008780:Methyltransferases / Q000235:genetics*; D019853:O(6)-Methylguanine-DNA Methyltransferase; D010947:Plants, Toxic; D010957:Plasmids / Q000235:genetics; D014026:Nicotiana / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics / Q000254:growth & development; D014162:Transfection / Q000235:genetics
chemical_list: D000477:Alkylating Agents; D008780:Methyltransferases; D019853:O(6)-Methylguanine-DNA Methyltransferase