Flow cytometry of oesophageal mucosal biopsies; epidermal growth factor receptor, and CD15.
Flow cytometry may be used to examine the properties of single or isolated cells. We have shown that it is possible to disaggregate and label oesophageal epithelial cells for two surface markers, CD15 and epidermal growth factor receptor. We have previously demonstrated these markers in oesophageal squamous cells using immunoperoxidase techniques. These labelled disaggregated cells could then be measured by flow cytometry.
Hopwood
D
D
Department of Pathology, Ninewells Hospital and Medical School, Dundee, U.K.
Jankowski
J
J
Milne
G
G
Wormsley
K G
KG
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015703:Antigens, CD / Q000378:metabolism*; D000954:Antigens, Surface / Q000378:metabolism; D001706:Biopsy; D000939:Epitopes; D066246:ErbB Receptors / Q000378:metabolism*; D004947:Esophagus / Q000378:metabolism / Q000473:pathology*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007150:Immunohistochemistry; D016256:Lewis X Antigen; D008854:Microscopy, Electron; D008875:Middle Aged; D009092:Mucous Membrane / Q000378:metabolism / Q000473:pathology; D012016:Reference Values
D015703:Antigens, CD; D000954:Antigens, Surface; D000939:Epitopes; D016256:Lewis X Antigen; D066246:ErbB Receptors