An ovarian tumor marker with homology to vaccinia virus contains an IgV-like region and multiple transmembrane domains.
The monoclonal antibody OVTL3 has a highly restricted reactivity with ovarian carcinomas and defines a surface glycoprotein, OA3, which has been used for immunotargeting. To understand why OA3 is found on ovarian tumors we isolated a complementary DNA by expression cloning. The clone encodes a 323-amino acid protein with 5 putative membrane spanning domains, reminiscent of a membrane receptor or channel, but of a new type with little or no sequence similarity with these families of proteins. Interestingly, the OA3 sequence is highly related to a vaccinia virus encoded protein (VA38) and its extracellular domain is a member of the immunoglobulin V region superfamily.
Campbell
I G
IG
Human Immunogenetics Laboratory, Imperial Cancer Research Fund, Lincoln's Inn Fields, London, United Kingdom.
Freemont
P S
PS
Foulkes
W
W
Trowsdale
J
J
D000595:Amino Acid Sequence; D000951:Antigens, Neoplasm / Q000235:genetics*; D001483:Base Sequence; D014408:Biomarkers, Tumor / Q000235:genetics*; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004273:DNA, Neoplasm / Q000235:genetics / Q000302:isolation & purification; D004274:DNA, Recombinant / Q000235:genetics; D005260:Female; D014644:Genetic Variation / Q000235:genetics; D006801:Humans; D007135:Immunoglobulin Variable Region / Q000235:genetics*; D008565:Membrane Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D010051:Ovarian Neoplasms / Q000235:genetics* / Q000276:immunology; D012326:RNA Splicing; D012333:RNA, Messenger / Q000235:genetics; D017385:Sequence Homology; D014407:Tumor Cells, Cultured; D014616:Vaccinia virus / Q000235:genetics*
D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D004273:DNA, Neoplasm; D004274:DNA, Recombinant; D007135:Immunoglobulin Variable Region; D008565:Membrane Proteins; D012333:RNA, Messenger