title: Stimulation of synovial fluid mononuclear cells with the human 65-kD heat shock protein or with live enterobacteria leads to preferential expansion of TCR-gamma delta+ lymphocytes.
abstract: T lymphocyte responses to heterologous or self 65-kD heat shock protein (hsp) have been implicated in the pathogenesis of various forms of arthritis. To delineate the relationship of 65-kD hsp to different synovial fluid (SF) T cell subsets, we stimulated synovial fluid (SFMC) and peripheral blood mononuclear cells (PBMC) from patients with different inflammatory rheumatic diseases and from healthy controls with human or mycobacterial 65-kD hsp, tetanus toxoid (TT), heat-killed or live Yersinia enterocolitica. Phenotyping of the resulting T cell lines revealed an increase of up to 97% TCR-gamma delta+ lymphocytes in the 65-kD hsp-stimulated SF-derived lines. This expansion of TCR-gamma delta+ cells was less pronounced with cultures of PBMC. A preferential expansion of TCR-gamma delta+ cells was also shown after SFMC stimulation with live, but not with heat-killed Yersinia or with TT. We conclude that a common mechanism is involved in the selective expansion of TCR-gamma delta+ lymphocytes upon SFMC infection with live Yersinia or upon contact with 65-kD hsp. Out of a panel of TCR-gamma delta+ T lymphocyte clones (TLC) derived from a human 65-kD hsp-stimulated line, only a minority of TLC proliferated weakly upon restimulation with this antigen in the presence of autologous monocytes, whereas TCR-alpha beta+ TLC responded vigorously to the human 65-kD hsp and in some cases also cross-recognized the mycobacterial hsp homologue and/or heat-killed Yersinia. This implies that additional factors or cells may be present in the milieu of SFMC cultures that propagate the expansion of TCR-gamma delta+ cells in response to 65-kD hsp or live bacteria.
authors.affiliation: First Department of Medicine, Johannes Gutenberg University, Mainz, Germany.
authors.forename: E
authors.initials: E
authors.lastname: Hermann
authors.forename: A W
authors.initials: AW
authors.lastname: Lohse
authors.forename: W J
authors.initials: WJ
authors.lastname: Mayet
authors.forename: R
authors.initials: R
authors.lastname: van der Zee
authors.forename: W
authors.initials: W
authors.lastname: Van Eden
authors.forename: P
authors.initials: P
authors.lastname: Probst
authors.forename: T
authors.initials: T
authors.lastname: Poralla
authors.forename: K H
authors.initials: KH
authors.lastname: Meyer zum Büschenfelde
authors.forename: B
authors.initials: B
authors.lastname: Fleischer
mesh_terms: D002460:Cell Line; D002999:Clone Cells; D004755:Enterobacteriaceae / Q000502:physiology*; D006360:Heat-Shock Proteins / Q000494:pharmacology*; D006801:Humans; D008213:Lymphocyte Activation / Q000502:physiology; D009000:Monocytes / Q000187:drug effects*; D010641:Phenotype; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000032:analysis; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000032:analysis*; D013582:Synovial Fluid / Q000166:cytology*; D013601:T-Lymphocytes / Q000648:ultrastructure
chemical_list: D006360:Heat-Shock Proteins; D016693:Receptors, Antigen, T-Cell, alpha-beta; D016692:Receptors, Antigen, T-Cell, gamma-delta