Cell density-dependent expression of heparin-binding growth-associated molecule (HB-GAM, p18) and its down-regulation by fibroblast growth factors.
Heparin-binding growth-associated molecule (HB-GAM) is a developmentally regulated protein that is intensely expressed during the rapid postnatal growth phase of rat brain. The expression of HB-GAM studied in 12 cell lines was restricted to C6 rat glioma cell line and BALB/c 3T3 cells. In BALB/c 3T3 cells the expression of HB-GAM was enhanced in confluent and quiescent cell cultures. When the confluent cultures were treated with bFGF the expression of HB-GAM mRNA was strongly reduced and the protein disappeared rapidly from proliferating cells. The data presented suggest involvement of HB-GAM in cell differentiation phenomena rather than in cell proliferation.
Merenmies
J
J
Institute of Biotechnology, University of Helsinki, Finland.
D016475:3T3 Cells; D000818:Animals; D015153:Blotting, Western; D002352:Carrier Proteins / Q000096:biosynthesis* / Q000502:physiology; D002452:Cell Count; D002454:Cell Differentiation / Q000502:physiology; D002455:Cell Division / Q000502:physiology; D016207:Cytokines / Q000096:biosynthesis* / Q000502:physiology; D015536:Down-Regulation; D016220:Fibroblast Growth Factor 1 / Q000494:pharmacology*; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D006133:Growth Substances / Q000096:biosynthesis* / Q000235:genetics / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D014407:Tumor Cells, Cultured
D002352:Carrier Proteins; D016207:Cytokines; D006133:Growth Substances; D012333:RNA, Messenger; D016222:Fibroblast Growth Factor 2; D016220:Fibroblast Growth Factor 1; C066845:pleiotrophin