[Enteropathogenicity and enteropathogenic toxin production of Vibrio mimicus].
Enteropathogenicity and enteroreactive-toxins were examined in 66 strains of Vibrio mimicus and the following results were obtained. Frequencies of enteropathogenic strains judged by the result of suckling mouse tests were 11/13 (85%) for clinical isolates and 37/53 (70%) for fish or environmental isolates. Frequencies of preservation of cholera toxin gene and NAG-ST gene were 2 and 15%, respectively, for 48 enteropathogenic strains, and 0 and 6%, respectively, for 18 non-enteropathogenic strains. Frequencies of production of NAG-rTDH, FAF and hemolysin were 4, 63 and 100%, respectively, for 48 enteropathogenic strains, and 6, 50, and 100%, respectively, for 18 non-enteropathogenic strains. No correlation between serovar and enteropathogenicity was observed in the suckling mouse test. Six out of 12 enteropathogenic strains produced hemolysin in ligated rabbit ileal loop, while 1 out of 12 non-enteropathogenic strains did so under the same condition. A significant inhibition of fluid accumulation in the ligated rabbit ileal loop test with viable cells was noted in rabbits immunized with hemolysin of non-O1 V. cholerae. These results suggest that approximately two-thirds of environmental isolates are enteropathogenic and that hemolysin is the most important toxin in the enteropathogenic mechanism of V. mimicus strains.
Gyobu
Y
Y
Toyama Institute of Health.
Isobe
J
J
Kodama
H
H
Sato
S
S
Shimada
T
T
D000818:Animals; D003967:Diarrhea / Q000382:microbiology / Q000401:mortality; D004768:Enterotoxins / Q000096:biosynthesis*; D051379:Mice; D011817:Rabbits; D014733:Vibrio / Q000302:isolation & purification / Q000378:metabolism / Q000472:pathogenicity*
D004768:Enterotoxins; C069211:enterotoxin, Vibrio mimicus