title: Factors in ruminant colostrum that influence cell growth and murine IgE antibody responses.
abstract: Bovine colostrum was investigated as a source of biologically active molecules capable of stimulating the growth of mammalian cells in culture and modifying the immune response in a murine model. An extract prepared from bovine colostral whey by cation exchange and reversed-phase chromatography stimulated the growth of L6 rat myoblasts, Balb/c-3T3 mouse fibroblasts and BHK-21 baby hamster kidney cells with equal or greater potency than fetal bovine serum. Fractionation of the bovine colostral extract by gel-permeation chromatography in M-acetic acid identified a number of cell-growth factors for each cell type. Bovine colostral extract was compared with an ovine colostral whey preparation for its ability to modulate IgE antibody responses in mice. Doses of 8 and 4 mg/d of ovine colostral whey or bovine colostral extract specifically suppressed IgE antibody responses, whereas at lower doses suppression did not occur. We conclude that bovine colostrum contains cell-growth factors as well as immunomodulatory factors that are able to regulate the IgE response in a heterologous species.
authors.affiliation: CSIRO Division of Animal Health, Armidale, New South Wales, Australia.
authors.forename: D L
authors.initials: DL
authors.lastname: Watson
authors.forename: G L
authors.initials: GL
authors.lastname: Francis
authors.forename: F J
authors.initials: FJ
authors.lastname: Ballard
mesh_terms: D000818:Animals; D000941:Antigens / Q000276:immunology; D002417:Cattle; D002455:Cell Division; D002460:Cell Line; D003126:Colostrum / Q000737:chemistry* / Q000502:physiology; D006224:Cricetinae; D005347:Fibroblasts / Q000166:cytology; D006133:Growth Substances / Q000302:isolation & purification / Q000494:pharmacology*; D007073:Immunoglobulin E / Q000096:biosynthesis*; D007668:Kidney / Q000166:cytology; D051379:Mice; D008807:Mice, Inbred BALB C; D008894:Milk Proteins / Q000494:pharmacology; D009132:Muscles / Q000166:cytology; D010047:Ovalbumin / Q000276:immunology; D051381:Rats; D000067816:Whey Proteins
chemical_list: D000941:Antigens; D006133:Growth Substances; D008894:Milk Proteins; D000067816:Whey Proteins; D007073:Immunoglobulin E; D010047:Ovalbumin