title: Neutralization of myotoxic phospholipases A2 from the venom of the snake Bothrops asper by monoclonal antibodies.
abstract: The neutralization of two myotoxic phospholipases A2 from the venom of Bothrops asper, myotoxins I and II, by two murine monoclonal antibodies is reported. The monoclonal antibodies, MAb-3 and Mab-4, recognize different epitopes of the toxin. Both antibodies completely neutralized myotoxic activity of myotoxin I and crude venom. MAb-3 also completely neutralized myotoxic activity of myotoxin II, a lysine-49 phospholipase A2 isoform, whereas MAb-4 neutralized this toxin only partially. MAb-3 neutralized myotoxin II at a molar ratio of 1:1, showing a higher efficiency than affinity-purified polyclonal antibodies. A dissociation of myotoxic and enzymatic activities of myotoxin I was observed with both monoclonal antibodies.
authors.affiliation: Instituto Clodomiro Picado, Facultad de Microbiologia, Universidad de Costa Rica, San Joseé.
authors.forename: B
authors.initials: B
authors.lastname: Lomonte
authors.forename: J M
authors.initials: JM
authors.lastname: Gutiérrez
authors.forename: M
authors.initials: M
authors.lastname: Ramírez
authors.forename: C
authors.initials: C
authors.lastname: Díaz
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D003435:Crotalid Venoms / Q000276:immunology* / Q000633:toxicity; D000939:Epitopes / Q000276:immunology; D054501:Group II Phospholipases A2; D051379:Mice; D008807:Mice, Inbred BALB C; D009498:Neurotoxins / Q000276:immunology* / Q000633:toxicity; D009500:Neutralization Tests; D010741:Phospholipases A / Q000276:immunology* / Q000633:toxicity; D054467:Phospholipases A2; D030162:Reptilian Proteins
chemical_list: D000911:Antibodies, Monoclonal; D003435:Crotalid Venoms; D000939:Epitopes; D009498:Neurotoxins; D030162:Reptilian Proteins; D010741:Phospholipases A; D054501:Group II Phospholipases A2; D054467:Phospholipases A2; C076780:myotoxin I; C061056:myotoxin II, Bothrops asper