[Total chemical synthesis of natural transfer RNA].
New improvements in the chemical synthesis of oligoribonucleotides are reported and they are applied to the first total chemical synthesis of a natural RNA. This E. coli K12 alanine tRNA contains in its sequence dihydrouridine, ribothymidine and pseudo-uridine. The synthetic tRNA was fully sequenced and showed a 42% aminoacyl acceptance activity. When tRNA was used as a template, reverse transcriptase directed the incorporation of adenine opposite dihydrouridine, ribothymidine and pseudouridine.
Gasparutto
D
D
Laboratoire de Radiobiochimie, S.E.S.A.M./D.R.F.M.C., Grenoble.
Livache
T
T
Duplaa
A M
AM
Bazin
H
H
Favario
S
S
Guy
A
A
Molko
D
D
Roget
A
A
Teoule
R
R
D001483:Base Sequence; D008969:Molecular Sequence Data; D011560:Pseudouridine; D012344:RNA, Transfer, Ala / Q000138:chemical synthesis*; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D014529:Uridine / Q000031:analogs & derivatives
D012344:RNA, Transfer, Ala; D011560:Pseudouridine; C009182:ribothymidine; D012194:RNA-Directed DNA Polymerase; D014529:Uridine