title: Branched-chain amino acid biosynthesis genes in Lactococcus lactis subsp. lactis.
abstract: The genes for biosynthesis of the branched-chain amino acids leucine, isoleucine, and valine in Lactococcus lactis subsp. lactis NCDO2118 were characterized by cloning, complementation in Escherichia coli and Bacillus subtilis, and nucleotide sequence analysis. Nine structural genes are clustered on a 12-kb DNA fragment in the order leuABCD ilvDBNCA. Upstream of these genes, the nucleotide sequence suggests the existence of regulation by transcriptional attenuation. Between the leuD and ilvD genes is an unexpected gene, encoding a protein which belongs to the ATP-binding cassette protein superfamily.
authors.affiliation: Laboratoire de Génétique Microbienne, Institut National de la Recherche Agronomique, Jouy-en-Josas, France.
authors.forename: J J
authors.initials: JJ
authors.lastname: Godon
authors.forename: M C
authors.initials: MC
authors.lastname: Chopin
authors.forename: S D
authors.initials: SD
authors.lastname: Ehrlich
mesh_terms: D000595:Amino Acid Sequence; D000597:Amino Acids, Branched-Chain / Q000096:biosynthesis / Q000235:genetics*; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D005798:Genes, Bacterial / Q000235:genetics*; D005816:Genetic Complementation Test; D013294:Lactococcus lactis / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics*; D009690:Nucleic Acid Conformation; D016366:Open Reading Frames; D010957:Plasmids / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics
chemical_list: D000597:Amino Acids, Branched-Chain; D001426:Bacterial Proteins; D012333:RNA, Messenger