Production and specificity of anti-22-oxacalcitriol antisera.
22-Oxacalcitriol 3-hemiglutarate, a haptenic derivative of 22-oxacalcitriol, was synthesized to obtain a specific antibody for use in radioimmunoassay. Three antisera were elicited in rabbits against the hapten conjugated with bovine serum albumin, and their specificity was examined by cross-reaction study. One of the antisera was found to be satisfactorily specific, and expected to provide a radioimmunoassay useful for the pharmacokinetic study of 22-oxacalcitriol.
Kobayashi
N
N
Faculty of Pharmaceutical Sciences, Kanazawa University, Japan.
Asano
T
T
Kitahori
J
J
Shimada
K
K
Kubodera
N
N
Watanabe
H
H
D000818:Animals; D000906:Antibodies / Q000032:analysis*; D000918:Antibody Specificity; D002117:Calcitriol / Q000031:analogs & derivatives* / Q000097:blood / Q000276:immunology; D005260:Female; D011817:Rabbits; D011863:Radioimmunoassay
D000906:Antibodies; C077050:22-oxacalcitriol-3-hemiglutarate; D002117:Calcitriol; C051883:maxacalcitol