DNA melting within stable closed complexes at the Escherichia coli rrnB P1 promoter.
Several transcription complexes are shown to form on the E. coli ribosomal rrnB P1 promoter in vitro. These include two closed complexes that are sensitive to heparin attack, and one open complex. The closed complexes are unusual in that they are both highly specific and stable, properties associated with the atypical DNA sequence of this promoter. The effector ppGpp does not prevent closed complex formation but does reduce the level of open complexes that form.
Ohlsen
K L
KL
Department of Chemistry and Biochemistry and The Molecular Biology Institute, University of California, Los Angeles 90024.
Gralla
J D
JD
D000255:Adenosine Triphosphate / Q000737:chemistry; D003570:Cytidine Triphosphate / Q000737:chemistry; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000235:genetics*; D004278:DNA, Superhelical / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D006159:Guanosine Tetraphosphate / Q000502:physiology; D010957:Plasmids; D011196:Potassium Permanganate / Q000737:chemistry; D011401:Promoter Regions, Genetic; D014158:Transcription, Genetic; D012407:rRNA Operon
D004269:DNA, Bacterial; D004278:DNA, Superhelical; D011196:Potassium Permanganate; D006159:Guanosine Tetraphosphate; D003570:Cytidine Triphosphate; D000255:Adenosine Triphosphate