Restoration of diabetes-induced changes in enteric nerves by phorbol 12,13-dibutyrate.
Treatment of segments of ileum from 8-week streptozotocin-induced diabetic rats with phorbol 12,13-dibutyrate (PDBu) in vitro resulted in restoration of the diabetes-induced changes in the expression of enteric VIP- and galanin-like immunoreactive nerve fibres. The increase in fluorescence intensity and density of VIP- and galanin-like immunoreactivity observed in 8-week streptozotocin-treated rats was reduced to a near normal level after 40 min incubation of diabetic tissues in Krebs solution containing PDBu (100 nM). The tissue content of VIP was also affected (control = 2.1 +/- 0.31 pmol/cm; diabetic = 4.6 +/- 0.48 pmol/cm; diabetic + PDBu = 2.9 +/- 0.91 pmol/cm) after incubation with PDBu. The significance of these findings in relation to the possible role of protein kinase C in the regulation of expression and/or storage of these enteric neuropeptides in normal and diabetic states is discussed.
Belai
A
A
Department of Anatomy and Developmental Biology, University College London, UK.
Burnstock
G
G
D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism / Q000473:pathology*; D019004:Galanin; D008297:Male; D009197:Myenteric Plexus / Q000737:chemistry / Q000473:pathology*; D010455:Peptides / Q000032:analysis; D015240:Phorbol 12,13-Dibutyrate / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D014660:Vasoactive Intestinal Peptide / Q000032:analysis*
D010455:Peptides; D014660:Vasoactive Intestinal Peptide; D015240:Phorbol 12,13-Dibutyrate; D019004:Galanin