title: Alterations in lens permeability during galactose cataract development in rat.
abstract: Lens permeability has been shown to be compromised during galactose-induced cataract development in rats. Recent studies have demonstrated permeability and diffusion pathways as well as endocytotic activity in normal lenses of different species using tracers of different molecular weights. We investigated the permeability and diffusion of tracers in normal rat lenses and in lenses during cataract development using three different molecular weight tracers, lanthanum nitrate (LN, MW 430), ruthenium red (RR, MW 858.5) and horseradish peroxidase (HRP, MW 40.000) for this study. Sprague Dawley rats weighing 50gms were fed Purina Rat Chow with or without galactose. Our results, based on transmission electron microscopy, show that all tracers penetrated through the capsule and the basal portion of the intercellular spaces. While the diffusion of RR was restricted to the epithelial cell layer, LN and HRP were observed in intercellular spaces in cortical fiber cells. In the HRP "washout" studies, HRP could be readily removed from the intercellular spaces in the basal region in the epithelial cell layer. This and other observations suggest the presence of a barrier (tight-junction) in the apical region. Our studies also suggest an influx of tracers in the lens, specifically LN and HRP, through the equatorial region. The permeability of the tracers increased and endocytotic vesicles with tracers were often found associated with the lateral and basal membranes of epithelial cells in the galactose-fed rats at the precataractous and mature cataractous stages. This study provides further support for the presence of a tight-junction between epithelial cells and indicates the movement of tracers through the equatorial region. Moreover, it confirms previous observations that indicated alterations in lens permeability during galactose cataractogenesis.
authors.affiliation: Department of Biological Sciences Oakland University, Rochester, MI 48309-4401.
authors.forename: M J
authors.initials: MJ
authors.lastname: Johnson
authors.forename: N J
authors.initials: NJ
authors.lastname: Unakar
mesh_terms: D000818:Animals; D002386:Cataract / Q000378:metabolism* / Q000473:pathology; D002463:Cell Membrane Permeability; D004195:Disease Models, Animal; D004848:Epithelium / Q000378:metabolism / Q000648:ultrastructure; D005260:Female; D005690:Galactose; D006735:Horseradish Peroxidase / Q000493:pharmacokinetics; D007365:Intercellular Junctions / Q000378:metabolism / Q000648:ultrastructure; D007811:Lanthanum / Q000493:pharmacokinetics; D007903:Lens Capsule, Crystalline / Q000378:metabolism / Q000648:ultrastructure; D007908:Lens, Crystalline / Q000378:metabolism* / Q000648:ultrastructure; D051381:Rats; D011919:Rats, Inbred Strains; D012430:Ruthenium Red / Q000493:pharmacokinetics
chemical_list: D012430:Ruthenium Red; C016534:lanthanide nitrate; D007811:Lanthanum; D006735:Horseradish Peroxidase; D005690:Galactose