title: Autocrine role of insulin-like growth factor (IGF)-I in a human thyroid cancer cell line.
abstract: An established cell line (TC-cell, clone 78) derived from human thyroid papillary cancer cells was investigated for production of peptide growth factors. The cells had specific binding sites for insulin-like growth factor-I (IGF-I) and responded to this growth factor with increased proliferation. Culture medium conditioned by TC cells was found to contain insulin-like growth factor (IGF)-I and IGF-binding protein(s). Furthermore, reverse transcription-polymerase chain reaction revealed expression of IGF-I mRNA. When monoclonal antibody to IGF-I receptors (alpha IR3) was added, the growth of TC cells cultured in serum-free medium was significantly reduced. The growth rate of the cells was restored when the antibody was removed from the medium. These results strongly suggest that TC cells produce IGF-I, which is involved in the regulation of their own growth.
authors.affiliation: Department of Medicine, Tokyo Women's Medical College, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Onoda
authors.forename: E
authors.initials: E
authors.lastname: Ohmura
authors.forename: T
authors.initials: T
authors.lastname: Tsushima
authors.forename: Y
authors.initials: Y
authors.lastname: Ohba
authors.forename: N
authors.initials: N
authors.lastname: Emoto
authors.forename: O
authors.initials: O
authors.lastname: Isozaki
authors.forename: Y
authors.initials: Y
authors.lastname: Sato
authors.forename: K
authors.initials: K
authors.lastname: Shizume
authors.forename: H
authors.initials: H
authors.lastname: Demura
mesh_terms: D002291:Carcinoma, Papillary / Q000378:metabolism; D002352:Carrier Proteins / Q000096:biosynthesis; D002452:Cell Count / Q000187:drug effects; D004273:DNA, Neoplasm / Q000096:biosynthesis; D006801:Humans; D018969:Insulin-Like Growth Factor Binding Proteins; D007334:Insulin-Like Growth Factor I / Q000096:biosynthesis* / Q000494:pharmacology / Q000502:physiology; D016133:Polymerase Chain Reaction; D013964:Thyroid Neoplasms / Q000378:metabolism*; D014407:Tumor Cells, Cultured / Q000378:metabolism
chemical_list: D002352:Carrier Proteins; D004273:DNA, Neoplasm; D018969:Insulin-Like Growth Factor Binding Proteins; D007334:Insulin-Like Growth Factor I