title: Frequency of HLA-DPB1 alleles, including a novel DPB1 sequence, in the Northern Ireland population.
abstract: HLA-DPB1 allele frequencies in 150 unrelated normal individuals from Northern Ireland were determined using oligonucleotide typing methods. HLA-DPB1*0401 was the most common allele in the population possessed by 75.3% of subjects, followed by DPB1*0201 (20.7%). In addition to these alleles, only HLA-DPB1*0402, -DPB1*0301, and -DPB1*0501 were present in subjects at frequencies greater than 10%. The results in this study are in broad agreement with other Caucasoid studies, but there is regional and ethnic variation in HLA-DP allele frequencies. Three DPB1 alleles were found to be in linkage disequilibrium with HLA-DR antigens determined by RFLP, namely, DPB1*0101 with DRw17 (Dw24 associated) RFLP, DPB1*0501 with DRw13-Dw19 RFLP, and DPB1*1901 with DRw13-Dw18 (Dw25 associated) RFLP. One individual revealed a novel DPB1 pattern of probe reactivity, which following DNA sequencing was found to be HLA-DPB1*2001. To assess the system used and to compare consistency of results between laboratories, 62 cell lines were oligotyped for HLA-DP. The results revealed the system described here to be extremely accurate and showed excellent agreement of HLA-DP typing results for cell lines between laboratories.
authors.affiliation: Northern Ireland Tissue Typing Service, Belfast City Hospital.
authors.forename: D A
authors.initials: DA
authors.lastname: Savage
authors.forename: D
authors.initials: D
authors.lastname: Middleton
authors.forename: F
authors.initials: F
authors.lastname: Trainor
authors.forename: A
authors.initials: A
authors.lastname: Taylor
authors.forename: P G
authors.initials: PG
authors.lastname: McKenna
authors.forename: C
authors.initials: C
authors.lastname: Darke
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D001483:Base Sequence; D005787:Gene Frequency; D006682:HLA-DP Antigens / Q000235:genetics*; D059868:HLA-DP beta-Chains; D006650:Histocompatibility Testing; D006801:Humans; D007959:Lymphocyte Culture Test, Mixed; D008969:Molecular Sequence Data; D009660:Northern Ireland; D015345:Oligonucleotide Probes; D011110:Polymorphism, Genetic
chemical_list: D006682:HLA-DP Antigens; D059868:HLA-DP beta-Chains; C071170:HLA-DPB1 antigen; D015345:Oligonucleotide Probes