Zinc causes tyrosine phosphorylation of hippocampal p60c-src.
Zinc cations at concentrations of 0.2 mM and greater catalyzed specific phosphorylation, by ATP, of two membrane-associated proteins from rat hippocampus. These proteins, corresponding to molecular weights of 60 and 49 kDa, were phosphorylated primarily at tyrosine residues. The 60-kDa protein was identified as p60c-src by immunoprecipitation using two different p60src-specific monoclonal antibodies. The 49-kDa protein co-immunoprecipitated with p60c-src. Cyanogen bromide cleavage of p60c-src and the 49-kDa protein phosphorylated in the presence of Zn2+ gave different patterns of phosphopeptides. It is suggested that tyrosine phosphorylation of p60c-src and the p60c-src-associated 49-kDa protein may be a way of zinc participation in hippocampal neurotransmission.
Vener
A V
AV
INSERM U29, Paris, France.
Loeb
J
J
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D003488:Cyanogen Bromide; D006624:Hippocampus / Q000378:metabolism*; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism; D010766:Phosphorylation; D011233:Precipitin Tests; D016392:Proto-Oncogene Proteins pp60(c-src) / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D014443:Tyrosine / Q000378:metabolism*; D014638:Vanadates / Q000494:pharmacology; D015032:Zinc / Q000502:physiology*
D008565:Membrane Proteins; D014638:Vanadates; D014443:Tyrosine; D000255:Adenosine Triphosphate; D016392:Proto-Oncogene Proteins pp60(c-src); D015032:Zinc; D003488:Cyanogen Bromide