Neutralization of myotoxic phospholipases A2 from the venom of the snake Bothrops asper by monoclonal antibodies.
The neutralization of two myotoxic phospholipases A2 from the venom of Bothrops asper, myotoxins I and II, by two murine monoclonal antibodies is reported. The monoclonal antibodies, MAb-3 and Mab-4, recognize different epitopes of the toxin. Both antibodies completely neutralized myotoxic activity of myotoxin I and crude venom. MAb-3 also completely neutralized myotoxic activity of myotoxin II, a lysine-49 phospholipase A2 isoform, whereas MAb-4 neutralized this toxin only partially. MAb-3 neutralized myotoxin II at a molar ratio of 1:1, showing a higher efficiency than affinity-purified polyclonal antibodies. A dissociation of myotoxic and enzymatic activities of myotoxin I was observed with both monoclonal antibodies.
Lomonte
B
B
Instituto Clodomiro Picado, Facultad de Microbiologia, Universidad de Costa Rica, San Joseé.
Gutiérrez
J M
JM
Ramírez
M
M
Díaz
C
C
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D003435:Crotalid Venoms / Q000276:immunology* / Q000633:toxicity; D000939:Epitopes / Q000276:immunology; D054501:Group II Phospholipases A2; D051379:Mice; D008807:Mice, Inbred BALB C; D009498:Neurotoxins / Q000276:immunology* / Q000633:toxicity; D009500:Neutralization Tests; D010741:Phospholipases A / Q000276:immunology* / Q000633:toxicity; D054467:Phospholipases A2; D030162:Reptilian Proteins
D000911:Antibodies, Monoclonal; D003435:Crotalid Venoms; D000939:Epitopes; D009498:Neurotoxins; D030162:Reptilian Proteins; D010741:Phospholipases A; D054501:Group II Phospholipases A2; D054467:Phospholipases A2; C076780:myotoxin I; C061056:myotoxin II, Bothrops asper