title: A chondroitin sulfate proteoglycan that is developmentally regulated in the cerebellar mossy fiber system.
abstract: It is known that the mammalian brain contains many kinds of proteoglycans, but almost all of them remain to be characterized. In this study, we prepared a monoclonal antibody against a phosphate-buffered saline-soluble brain proteoglycan (MAb 6B4). MAb 6B4 recognized a 600- to 1000-kDa chondroitin sulfate proteoglycan with a 250-kDa core protein (6B4 proteoglycan). The core protein of 6B4 proteoglycan carried the HNK-1 epitope. Immunohistochemical analysis of the adult rat brain indicated that this proteoglycan was expressed on the cell surfaces of a subset of neurons. In the hindbrain, 6B4 proteoglycan was highly expressed on the cerebellar Purkinje cells and Golgi cells, and at particular nuclei including the pontine nuclei and lateral reticular nucleus. Almost all of these nuclei were connected to the cerebellum through the mossy fiber system. A developmental study indicated that the expression of this proteoglycan changed dramatically during the formation of the cerebellar mossy fiber system. The mossy fibers from the pontine nuclei expressed 6B4 proteoglycan transiently from Embryonic Day 20 (E20) to Postnatal Day 30 (P30), during which time the axonal outgrowth and glomerular synapse formation occurred. The Purkinje cells, glomeruli, and Golgi cells began to be stained with MAb 6B4 from P10, P16, and P20, respectively. These expression stages correspond with the onset of their synapse formation. These results suggest that 6B4 proteoglycan is closely involved in the development of the cerebellar mossy fiber system.
authors.affiliation: Department of Embryology, Institute for Developmental Research, Aichi, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Maeda
authors.forename: F
authors.initials: F
authors.lastname: Matsui
authors.forename: A
authors.initials: A
authors.lastname: Oohira
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000737:chemistry; D002531:Cerebellum / Q000737:chemistry / Q000254:growth & development* / Q000378:metabolism; D011508:Chondroitin Sulfate Proteoglycans / Q000276:immunology / Q000502:physiology*; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D009412:Nerve Fibers / Q000737:chemistry / Q000502:physiology*; D051381:Rats; D011919:Rats, Inbred Strains; D012249:Rhombencephalon / Q000737:chemistry; D013194:Staining and Labeling
chemical_list: D000911:Antibodies, Monoclonal; D011508:Chondroitin Sulfate Proteoglycans