Removal of endotoxin from culture supernatant of Bordetella pertussis with aminated poly(gamma-methyl L-glutamate) spherical beads.
Attempts were made to prepare adsorbents having a high affinity for endotoxin in the culture supernatant of Bordetella pertussis. When poly(gamma-methyl L-glutamate) (PMLG) was used as a matrix and amino groups as the ligand, the highest affinity for endotoxin was attained even at a high ionic strength (mu = 0.2-0.4). PMLG beads containing amino groups of about 3.2 meq/g selectively removed endotoxin from the culture supernatant of B. pertussis without affecting the protective antigens. It was demonstrated that 1 ml of the wet adsorbent adsorbed 4.5 mg of endotoxin. The beads of PMLG derivatives, therefore, are considered to be a useful adsorbent for the removal of endotoxin from the pertussis vaccine, affecting neither filamentous hemagglutinin nor pertussis toxin.
Hirayama
C
C
Department of Applied Chemistry, Faculty of Engineering, Kumamoto University, Japan.
Sakata
M
M
Ohkura
Y
Y
Ihara
H
H
Ohkuma
K
K
D000327:Adsorption; D001886:Bordetella pertussis / Q000737:chemistry*; D004731:Endotoxins / Q000302:isolation & purification*; D004797:Enzyme-Linked Immunosorbent Assay; D008863:Microspheres; D011099:Polyglutamic Acid / Q000031:analogs & derivatives
D004731:Endotoxins; C030315:poly-gamma-methylglutamate; D011099:Polyglutamic Acid