Arginine transport in mitochondria of Neurospora crassa.
Transport of arginine into mitochondria of Neurospora crassa has been studied. Arginine transport was found to be saturable (Km = 6.5 mM) and to have a pH optimum of pH 7.5. Mitochondrial arginine transport appeared to be facilitated transport rather than active transport because: (i) the arginine concentration within the mitochondrial matrix after transport was similar to that of the reaction medium, and (ii) uncouplers and substrates of oxidative phosphorylation did not affect the transport rate. The basic amino acids ornithine, lysine, and D-arginine inhibited arginine transport. The arginine transport system could be irreversibly blocked by treating mitochondria with the reactive arginine derivative, N-nitrobenzyloxycarbonyl-arginyl diazomethane.
Yu
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YG
Department of Chemistry and Biochemistry, University of California, Los Angeles 90024-1569.
Weiss
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RL
D000596:Amino Acids / Q000494:pharmacology; D001120:Arginine / Q000378:metabolism*; D001692:Biological Transport / Q000187:drug effects; D002458:Cell Fractionation; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism*; D009492:Neurospora crassa / Q000378:metabolism*; D013395:Sucrose / Q000494:pharmacology
D000596:Amino Acids; D013395:Sucrose; D001120:Arginine