New DNA repair-deficient mutants of Chlamydomonas reinhardtii.
Two new UV-sensitive mutants of Chlamydomonas reinhardtii, uvs12 and uvs13, were characterized. Genetic analysis proved that they were non-allelic. They complemented the other repair-deficient mutants uvs8, uvs9, uvs10 and uvs11. While uvs12 may have an impaired excision-repair pathway, uvs13 is, by its UV sensitivity under non-photoreactivating conditions, very similar to uvsE1 and uvs10, but differs in the effect of caffeine on its survival. After UV, survival of some repair-deficient mutants was, under photoreactivating conditions, much lower than that of phr1, while survival of other repair-deficient mutants did not differ from that of a wild-type strain. A lower UV survival of some dark-repair-defective mutants of Chlamydomonas reinhardtii, under photoreactivating conditions, can perhaps be used as an additional criterion for mutants defective in the excision-repair pathway.
Podstavková
S
S
Department of Genetics, Faculty of Science, Comenius University, Bratislava, Czechoslovakia.
Vlcek
D
D
Miadoková
E
E
D000818:Animals; D002110:Caffeine / Q000494:pharmacology; D016825:Chlamydomonas reinhardtii / Q000235:genetics*; D004247:DNA / Q000187:drug effects / Q000528:radiation effects; D004260:DNA Repair; D004307:Dose-Response Relationship, Radiation; D005816:Genetic Complementation Test; D009154:Mutation; D014466:Ultraviolet Rays
D002110:Caffeine; D004247:DNA