A comparison of two autoradiographic methods for detecting radiolabeled nucleic acids in embryos.
Two methods for preparing embryos for autoradiographic study of newly synthesized nucleic acids are described and compared. The first method consists of rapidly fixing radiolabeled embryos with acetic acid:methanol, spreading them on glass slides and exposing them for 8 days with a photographic emulsion. The second method consists of fixing, embedding in resin, and sectioning the embryos before their exposure with the emulsion for 3 weeks. Both techniques have many applications in studies of early embryonic activity, but the spread technique is very sensitive, simpler, and faster.
Plante
L
L
Department of Biomedical Sciences, University of Guelph, Ontario, Canada.
Pollard
J W
JW
King
W A
WA
D000818:Animals; D001345:Autoradiography / Q000379:methods*; D001755:Blastocyst / Q000737:chemistry* / Q000648:ultrastructure; D002417:Cattle / Q000196:embryology; D004247:DNA / Q000032:analysis* / Q000096:biosynthesis; D012313:RNA / Q000032:analysis* / Q000096:biosynthesis; D013048:Specimen Handling
D012313:RNA; D004247:DNA