Neutrophil priming by hepatocyte growth factor, a novel cytokine.
We demonstrate here that the recently defined cytokine hepatocyte growth factor (HGF) 'primes' human neutrophils. Recombinant human HGF over the concentration range 0.1-20 ng/ml increased the neutrophil response to f-met-leu-phe by up to 200%, and required only a short preincubation, 10 min producing the maximum effect. Priming was independent of changes in cytosolic-free calcium homeostasis. We conclude that HGF may be a physiologically important cytokine with 'priming' activity for neutrophils.
Jiang
W
W
University Department of Surgery, University of Wales College of Medicine, Cardiff, U.K.
Puntis
M C
MC
Nakamura
T
T
Hallett
M B
MB
D002478:Cells, Cultured; D004306:Dose-Response Relationship, Immunologic; D017228:Hepatocyte Growth Factor / Q000276:immunology*; D006801:Humans; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000276:immunology; D009504:Neutrophils / Q000276:immunology*; D011994:Recombinant Proteins / Q000276:immunology
D011994:Recombinant Proteins; D009240:N-Formylmethionine Leucyl-Phenylalanine; D017228:Hepatocyte Growth Factor