Immunogenicity of recombinant core particles of hepatitis B virus containing epitopes of human immunodeficiency virus 1 core antigen.
A Gag protein segment of human immunodeficiency virus 1 (HIV-1) has been fused to a C terminally truncated core antigen of hepatitis B virus (HBcAg) using an E. coli expression system. Fusion of 90 amino acids of HIV-1 Gag protein to HBcAg still allowed the formation of capsids presenting on their surface epitopes of HIV-1 core protein, whereas fusion of 317, 189, or 100 amino acids of Gag prevented self-assembly of chimeric particles. Mice immunized with recombinant particles emulsified with Freund's complete adjuvant (CFA) or aluminium hydroxide developed high anti-HBcAg titers. However, anti-HIVp24 antibodies were detected only in mice inoculated with immunogen emulsified with CFA.
Ulrich
R
R
Institute of Virology, Charité, Berlin, Federal Republic of Germany.
Borisova
G P
GP
Gren
E
E
Berzin
I
I
Pumpen
P
P
Eckert
R
R
Ose
V
V
Siakkou
H
H
Gren
E J
EJ
von Baehr
R
R
D016915:AIDS Vaccines / Q000235:genetics / Q000276:immunology; D000818:Animals; D015153:Blotting, Western; D000939:Epitopes / Q000235:genetics / Q000276:immunology; D004926:Escherichia coli / Q000235:genetics; D005620:Freund's Adjuvant; D015483:HIV Antibodies / Q000096:biosynthesis*; D016655:HIV Core Protein p24 / Q000235:genetics / Q000276:immunology*; D015497:HIV-1 / Q000235:genetics / Q000276:immunology; D006510:Hepatitis B Antibodies / Q000096:biosynthesis*; D006512:Hepatitis B Core Antigens / Q000235:genetics / Q000276:immunology*; D017325:Hepatitis B Vaccines; D006515:Hepatitis B virus / Q000235:genetics / Q000276:immunology; D006801:Humans; D051379:Mice; D008854:Microscopy, Electron; D010957:Plasmids / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology* / Q000648:ultrastructure; D014614:Vaccines, Synthetic / Q000235:genetics / Q000276:immunology; D014761:Viral Hepatitis Vaccines / Q000235:genetics / Q000276:immunology
D016915:AIDS Vaccines; D000939:Epitopes; D015483:HIV Antibodies; D016655:HIV Core Protein p24; D006510:Hepatitis B Antibodies; D006512:Hepatitis B Core Antigens; D017325:Hepatitis B Vaccines; D011993:Recombinant Fusion Proteins; D014614:Vaccines, Synthetic; D014761:Viral Hepatitis Vaccines; D005620:Freund's Adjuvant