Identification of Brucella spp. by using the polymerase chain reaction.
The application of two synthetic oligonucleotides as probes and as primers in the polymerase chain reaction is presented for a specific, sensitive, and quick identification of Brucella spp. The specific oligonucleotide sequences were chosen on the basis of a 16S rRNA sequence alignment between Brucella abortus and Agrobacterium tumefaciens.
Herman
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L
Rijkszuivelstation, Centrum voor Landbouwkundig Onderzoek, Melle, Belgium.
De Ridder
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H
D001483:Base Sequence; D002002:Brucella / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D015342:DNA Probes; D004269:DNA, Bacterial / Q000235:genetics; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
D015342:DNA Probes; D004269:DNA, Bacterial; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S