title: Bioavailability and biological activity of wheat-bound chlorpyrifos-methyl residues in rats.
abstract: Wheat grain was treated with 14C-chlorpyrifos-methyl to generate bound residues for determining their bioavailability to rats. In a parallel experiment, bound residues were prepared with non-labelled chlorpyrifos-methyl to determine possible adverse effects in rats fed the grain-bound residue for 28 and 90 days. Two dose levels of 10 and 50 ppm were initially used on the grain. The 10 ppm led to the formation of 25.1% bound residues (2.51 ppm) after 6 months as determined by radiomeasurement. The higher dose was assumed to form 12.55 ppm bound residues. When 14C-bound residues were fed to male rats for 24 hours, the animals eliminated 75% of the radioactivity in urine, 7% in expired air and 8% in faeces after 3 days, indicating that the bound residues were highly bioavailable. A further "bioavailable" amount (4%) was found in selected organs.
authors.affiliation: Institute of Pesticides and Environmental Protection, Zemun-Belgrade, Yugoslavia.
authors.forename: N K
authors.initials: NK
authors.lastname: Neskovic
authors.forename: V
authors.initials: V
authors.lastname: Karan
authors.forename: M
authors.initials: M
authors.lastname: Budimir
authors.forename: V
authors.initials: V
authors.lastname: Vojinovic
authors.forename: S
authors.initials: S
authors.lastname: Gasic
authors.forename: S L
authors.initials: SL
authors.lastname: Vitorovic
mesh_terms: D000410:Alanine Transaminase / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D001682:Biological Availability; D004390:Chlorpyrifos / Q000031:analogs & derivatives* / Q000032:analysis / Q000493:pharmacokinetics / Q000633:toxicity; D002802:Cholinesterases / Q000187:drug effects / Q000378:metabolism; D005260:Female; D005506:Food Contamination / Q000032:analysis; D008297:Male; D009929:Organ Size / Q000187:drug effects; D010573:Pesticide Residues / Q000032:analysis / Q000493:pharmacokinetics* / Q000633:toxicity; D051381:Rats; D011919:Rats, Inbred Strains; D014908:Triticum
chemical_list: D010573:Pesticide Residues; D000410:Alanine Transaminase; D002802:Cholinesterases; D004390:Chlorpyrifos; C007031:chlorpyrifos-methyl