title: Cloning and characterisation of an oleosin gene from Brassica napus.
abstract: The sequence of an oleosin gene from Brassica napus has been determined. This gene contains a single intron of 437 bp and encodes a polypeptide of 195 amino acids. The oleosin gene product has an estimated molecular mass of 21.5 kDa and consists of a highly hydrophobic central domain flanked by relatively polar N- and C-terminal domains. The central domain is highly conserved between all oleosins sequenced to date and contains a run of periodically spaced leucine residues similar to that of a leucine-zipper motif. The gene has been shown to be expressed specifically in the embryo, maximally between 9 and 11 weeks after flowering, i.e. during the seed desiccation stage. Two transcriptional start sites have been mapped to -70 and -21 of the ATG and a putative ABA-responsive element and three repeated motifs have been identified in the promoter. These short promoter sequences could correspond to regulatory elements responsible for embryo-specific gene expression. Up to six genes exist in the oleosin gene family.
authors.affiliation: Department of Brassica and Oilseeds Research, Cambridge Laboratory, John Innes Centre for Plant Science Research, Norwich, UK.
authors.forename: J S
authors.initials: JS
authors.lastname: Keddie
authors.forename: G
authors.initials: G
authors.lastname: Hübner
authors.forename: S P
authors.initials: SP
authors.lastname: Slocombe
authors.forename: R P
authors.initials: RP
authors.lastname: Jarvis
authors.forename: I
authors.initials: I
authors.lastname: Cummins
authors.forename: E W
authors.initials: EW
authors.lastname: Edwards
authors.forename: C H
authors.initials: CH
authors.lastname: Shaw
authors.forename: D J
authors.initials: DJ
authors.lastname: Murphy
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D001937:Brassica / Q000235:genetics*; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D008074:Lipoproteins / Q000235:genetics*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009838:Oligodeoxyribonucleotides; D010940:Plant Proteins / Q000235:genetics*; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
chemical_list: D008074:Lipoproteins; D009838:Oligodeoxyribonucleotides; D010940:Plant Proteins; C079356:oleosin protein, Brassica napus; D012313:RNA; D004247:DNA