Identification of a small epitope in domain Ib of Pseudomonas aeruginosa exotoxin A that elicits enzyme-neutralizing antibodies.
A peptide corresponding to amino acids 392-404 of the amino acid sequence of Pseudomonas aeruginosa exotoxin A (the last 13 amino acids of domain Ib) was synthesized and coupled to thyroglobulin. The conjugate induced an antiserum in rabbits with high antibody titer against native toxin as measured by ELISA, and this antiserum was highly efficient in inhibiting the ADP-ribosyltransferase activity of exotoxin A. These data corroborate the potential importance of amino acids 400-404 in the enzymatic mechanism of exotoxin A.
Rutault
K
K
Laboratoire de Biochimie Microbienne, Université Claude Bernard Lyon I, Villeurbanne, France.
Coin
D
D
Vacheron
M J
MJ
Guinand
M
M
Wallach
J
J
Michel
G
G
D036002:ADP Ribose Transferases; D000595:Amino Acid Sequence; D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology*; D000942:Antigens, Bacterial / Q000276:immunology*; D001427:Bacterial Toxins; D000939:Epitopes / Q000276:immunology*; D005098:Exotoxins / Q000276:immunology*; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000276:immunology*; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; D011065:Poly(ADP-ribose) Polymerases / Q000276:immunology*; D011550:Pseudomonas aeruginosa / Q000201:enzymology / Q000276:immunology*; D011817:Rabbits; D013954:Thyroglobulin; D037521:Virulence Factors; D000097668:Pseudomonas aeruginosa Exotoxin A
D036002:ADP Ribose Transferases; D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001427:Bacterial Toxins; D000939:Epitopes; D005098:Exotoxins; D010446:Peptide Fragments; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; D011065:Poly(ADP-ribose) Polymerases; D013954:Thyroglobulin; D037521:Virulence Factors; D000097668:Pseudomonas aeruginosa Exotoxin A