Effects of calcitonin on human trophoblastic cells in culture: absence of autocrine control.
The purpose of this work was to investigate the effects of calcitonin (CT) on trophoblastic cells with respect to cAMP levels and human chorionic gonadotrophin (hCG) secretion in cultured cells from first-trimester and term placentas and in a choriocarcinoma cell line (JEG-3). The expression of the CT gene was investigated to elucidate a putative autocrine control of CT during pregnancy. The addition of salmon CT (10(-10) M and above) resulted in concentration-dependent increases in cAMP secretion by normal trophoblastic cells from term and first-trimester placentas. Moreover, CT was found to increase cAMP secretion preferentially in completely differentiated cells, i.e. after 4-7 days in culture. Addition to the culture medium of JEG-3 cells slightly increased cAMP secretion only at a concentration of 10(-8) M. The basal level of hCG in the medium was found to be higher in the first-trimester than in the term trophoblast culture, but salmon CT induced an increase in hCG secretion by term placenta cells only. CT gene expression in our experimental model was investigated to elucidate a putative autocrine control of CT action during pregnancy. It was not found to be expressed in syncytiotrophoblast cells from either first-trimester or term placenta cells by the method used. Our data demonstrate the absence of autocrine control of CT effects in trophoblastic cells, and suggest that CT is likely to exert its effect preferentially on differentiated cells.
Rebut-Bonneton
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C
URA 1337 CNRS Laboratoire de Physiopathologie du Développement, Paris, France.
Segond
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N
Demignon
J
J
Porquet
D
D
Evain-Brion
D
D
D015152:Blotting, Northern; D002116:Calcitonin / Q000235:genetics / Q000494:pharmacology*; D002454:Cell Differentiation; D002478:Cells, Cultured; D002822:Choriocarcinoma / Q000378:metabolism; D006063:Chorionic Gonadotropin / Q000378:metabolism*; D000242:Cyclic AMP / Q000378:metabolism*; D005260:Female; D015870:Gene Expression; D006801:Humans; D009693:Nucleic Acid Hybridization; D011247:Pregnancy; D012313:RNA / Q000032:analysis; D014327:Trophoblasts / Q000187:drug effects* / Q000378:metabolism; D014407:Tumor Cells, Cultured
D006063:Chorionic Gonadotropin; D012313:RNA; C028815:salmon calcitonin; D002116:Calcitonin; D000242:Cyclic AMP