A prominent epitope on GABAA receptors is recognized by two different monoclonal antibodies.
The monoclonal antibody 62-3G1 raised against the GABAA/benzodiazepine receptor complex was tested for its subunit selectivity using recombinantly expressed GABAA receptor subunits. The antibody bound selectively to beta 2 and beta 3 but not beta 1 nor any other GABAA receptor subunit. Using enzyme-linked immunosorbent assay, the epitope on beta 2 and beta 3 subunits was determined to be residues 1-3. MAb bd 17, which displays identical subunit selectivity as mAb 62-3G1, was seen to bind to the same epitope. These results resolve the subunit selectivity of mAbs 62-3G1 and bd 17 and reveal the identity and localization of a prominent immunogenic epitope on the GABAA/benzodiazepine receptor.
Ewert
M
M
Laboratory of Molecular Neuroendocrinology, ZMBH, University of Heidelberg, F.R.G..
de Blas
A L
AL
Möhler
H
H
Seeburg
P H
PH
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D002461:Cell Line, Transformed; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000032:analysis*; D006801:Humans; D007668:Kidney; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D051381:Rats; D011963:Receptors, GABA-A / Q000032:analysis / Q000235:genetics / Q000276:immunology*; D012689:Sequence Homology, Nucleic Acid; D014162:Transfection
D000911:Antibodies, Monoclonal; D000939:Epitopes; D046911:Macromolecular Substances; D011963:Receptors, GABA-A