Heterogeneity in buffalo lutropin.
Lutropin (LH-1) from water buffaloes has been shown to exhibit microheterogeneity in the N-terminal amino-acid sequence of its alpha-subunit. The beta-subunit did not exhibit such microheterogeneity. Another protocol of purification yielded a preparation of buffalo LH (bu LH-2) different from the buffalo LH-1 in certain physico-chemical properties like ease of dissociation into subunits, sugar composition, isoelectric point, and elution profile on S-200. Data appear to indicate the presence of more than one form of buffalo lutropin.
Muralidhar
K
K
Department of Zoology, University of Delhi.
Rajendrakumar
T
T
Sharma
H P
HP
D000818:Animals; D002020:Buffaloes; D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000032:analysis; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D007986:Luteinizing Hormone / Q000737:chemistry* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry* / Q000302:isolation & purification; D010902:Pituitary Gland / Q000737:chemistry; D013464:Sulfuric Acids / Q000032:analysis
D002241:Carbohydrates; D009844:Oligosaccharides; D013464:Sulfuric Acids; D007986:Luteinizing Hormone