Similarity of copper and technetium binding sites in human IgG.
Kits for direct labeling of IgG with 99mTc were used without modification for the preparation of [67Cu]IgG. The IgG was pre-treated to generate thiolate groups which would bind 67Cu. The direct labeling of reduced IgG with 67Cu was highly efficient, resulting in approx. 95% 67Cu binding. Non-reduced IgG (negative control) had labeling efficiencies of less than 10%. IgG pre-exposed to Cu(II) had reduced amounts of 99mTc bound to it. The results demonstrate a direct relationship between copper- and 99mTc-binding sites in IgG.
Zamora
P O
PO
RhoMed Inc., Albuquerque, NM 87109.
Mercer-Smith
J A
JA
Marek
M J
MJ
Schulte
L D
LD
Rhodes
B A
BA
D001665:Binding Sites; D001667:Binding, Competitive; D002851:Chromatography, High Pressure Liquid; D003300:Copper / Q000378:metabolism*; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism*; D013667:Technetium / Q000378:metabolism*; D005719:gamma-Globulins / Q000378:metabolism
D007074:Immunoglobulin G; D005719:gamma-Globulins; D013667:Technetium; D003300:Copper