title: Characterization of antibodies against human N-terminal parathyroid hormone by epitope mapping.
abstract: Two polyclonal antisera from goat and mouse and two monoclonal antibodies against human parathyroid hormone (1-34) were characterised by epitope mapping. Hexapeptides were synthesized on polystyrene pins, the sequences of which overlapped and represented the entire sequence of hPTH(1-34). Binding of antibodies to these hexapeptides was determined and antigenic determinants thus characterized. At least one predominant binding sequence was detected in the region of hPTH(7-14).
authors.affiliation: Immundiagnostik, Bensheim, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Tampe
authors.forename: P
authors.initials: P
authors.lastname: Broszio
authors.forename: H E
authors.initials: HE
authors.lastname: Manneck
authors.forename: A
authors.initials: A
authors.lastname: Missbichler
authors.forename: E
authors.initials: E
authors.lastname: Blind
authors.forename: K B
authors.initials: KB
authors.lastname: Müller
authors.forename: H
authors.initials: H
authors.lastname: Schmidt-Gayk
authors.forename: F P
authors.initials: FP
authors.lastname: Armbruster
mesh_terms: D000595:Amino Acid Sequence; D000906:Antibodies; D000911:Antibodies, Monoclonal; D000939:Epitopes / Q000737:chemistry; D006801:Humans; D007120:Immunochemistry; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010281:Parathyroid Hormone / Q000737:chemistry / Q000276:immunology*; D010446:Peptide Fragments / Q000737:chemistry / Q000276:immunology*; D010449:Peptide Mapping; D011487:Protein Conformation; D019379:Teriparatide
chemical_list: D000906:Antibodies; D000911:Antibodies, Monoclonal; D000939:Epitopes; D010281:Parathyroid Hormone; D010446:Peptide Fragments; D019379:Teriparatide