Analysis of the human cysteine-rich protein gene (CSRP), assignment to chromosome 1q24-1q32, and identification of an associated MspI polymorphism.
The human cysteine-rich protein (hCRP) is encoded by a highly conserved and widely expressed serum-inducible immediate early response gene. hCRP contains two copies of the "LIM/double zinc-finger" motif. Using a characterized hCRP cDNA probe, we demonstrate that the human CRP gene (CSRP) is present in a single copy and that both mouse and human genomes contain one or more CRP-related genes detected by hybridization at low stringency. Using a panel of human x rodent somatic cell hybrids, the hCRP locus is assigned to chromosome 1. In situ hybridization of 3H-labeled CRP cDNA to human metaphase chromosomes confirms this assignment and permits regional localization to bands 1q24-1q32. A common MspI polymorphism is identified and mapped to intron 4 of the hCRP gene. The chromosomal localization and restriction site polymorphism should prove useful in future studies of the function of this gene.
Wang
X
X
Department of Medicine, Howard Hughes Medical Institute, University of Pennsylvania, Philadelphia 19104.
Ray
K
K
Szpirer
J
J
Levan
G
G
Liebhaber
S A
SA
Cooke
N E
NE
D000818:Animals; D015139:Blotting, Southern; D002874:Chromosome Mapping; D002878:Chromosomes, Human, Pair 1; D004247:DNA; D015342:DNA Probes; D019003:Deoxyribonuclease HpaII; D015252:Deoxyribonucleases, Type II Site-Specific; D005260:Female; D006801:Humans; D006822:Hybrid Cells; D017403:In Situ Hybridization; D060588:LIM Domain Proteins; D008297:Male; D051379:Mice; D009687:Nuclear Proteins; D010375:Pedigree; D012150:Polymorphism, Restriction Fragment Length; D011506:Proteins; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics*; D051381:Rats
C486177:Csrp1 protein, mouse; D015342:DNA Probes; D060588:LIM Domain Proteins; D009687:Nuclear Proteins; D011506:Proteins; D016271:Proto-Oncogene Proteins c-myc; C074370:cysteine-rich protein, mammalian; D004247:DNA; D019003:Deoxyribonuclease HpaII; D015252:Deoxyribonucleases, Type II Site-Specific