Isolation of a yeast artificial chromosome clone that spans the (12;16) translocation breakpoint characteristic of myxoid liposarcoma.
Cytogenetic analysis of liposarcomas has demonstrated that translocation (12;16) (q13.3;p11.2) is characteristic of the myxoid subtype of this adipose tissue tumor. Our previous results suggested that the GLI gene is close to the translocation breakpoint on chromosome 12. We now describe a yeast artificial chromosome (YAC) that contains GLI and spans the chromosome 12 region involved in the t(12;16) breakpoint. This clone will permit rapid definition of the genetic region surrounding the breakpoint and allow isolation of the gene presumably affected by the translocation.
Gemmill
R M
RM
Eleanor Roosevelt Institute, Denver, Colorado 80206.
Mendez
M J
MJ
Dougherty
C M
CM
Paulien
S
S
Liao
M
M
Mitchell
D
D
Jankowski
S A
SA
Trent
J M
JM
Berger
C
C
Sandberg
A A
AA
D015825:Chromosomes, Fungal; D002881:Chromosomes, Human, Pair 12; D002885:Chromosomes, Human, Pair 16; D003001:Cloning, Molecular; D015723:Gene Library; D015894:Genome, Human; D006801:Humans; D008080:Liposarcoma / Q000235:genetics*; D014178:Translocation, Genetic