Innervation of the paralyzed laryngeal muscles by phrenic motoneurons. A quantitative study by light and electron microscopy.
In the cat, inspiratory opening of the paralyzed glottis recovered after unilateral or bilateral reinnervation of the posterior cricoarytenoid (PCA) muscles by phrenic axons. The morphometric analysis of the regenerated recurrent laryngeal nerves (RLNs), showed that proliferation was abundant; 4 months after the nerve anastomosis, more than 500 myelinated axonal branches repopulated the RLNs. The mean diameter of motor axons (3.5 to 5.0 microns) was lower than in normal phrenic and RLN (8 to 10 microns), and the mean internode length was about half that of the normal RLN. Histochemical examination of the PCA muscle revealed that muscle fiber composition (44% type I and 56% type II muscle fiber) was fairly similar to that of normal PCA. The contraction time of the reinnervated muscles was as long as 60 msec at the time of movement recovery, but it shortened to 25 to 30 msec when the reinnervation time increased. These anatomical and functional results support the choice of the phrenic nerve for laryngeal reinnervation.
Baldissera
F
F
Institute of Human Physiology II, Milan, Italy.
Tredici
G
G
Marini
G
G
Fiori
M G
MG
Cantarella
G
G
Ottaviani
F
F
Zanoni
R
R
D000251:Adenosine Triphosphatases / Q000032:analysis; D000714:Anastomosis, Surgical; D000818:Animals; D001369:Axons / Q000502:physiology / Q000648:ultrastructure; D002415:Cats; D004576:Electromyography; D007821:Laryngeal Muscles / Q000294:innervation* / Q000473:pathology; D008854:Microscopy, Electron; D009046:Motor Neurons / Q000166:cytology / Q000502:physiology*; D009210:Myofibrils / Q000648:ultrastructure; D009411:Nerve Endings / Q000648:ultrastructure; D009412:Nerve Fibers / Q000648:ultrastructure; D009413:Nerve Fibers, Myelinated / Q000648:ultrastructure; D009416:Nerve Regeneration; D009469:Neuromuscular Junction / Q000502:physiology* / Q000648:ultrastructure; D010088:Oxidoreductases / Q000032:analysis; D010791:Phrenic Nerve / Q000473:pathology / Q000503:physiopathology / Q000601:surgery*; D011930:Reaction Time; D012009:Recurrent Laryngeal Nerve / Q000473:pathology / Q000503:physiopathology / Q000601:surgery; D014826:Vocal Cord Paralysis / Q000473:pathology / Q000601:surgery*
D010088:Oxidoreductases; D000251:Adenosine Triphosphatases