Intravascular circulation and distribution of human 51Cr-DBBF stroma-free hemoglobin.
Male B6C3HF1 mice were infused with 51Cr-labeled DBBF (bis 3,5-dibromosalicyl fumarate) crosslinked stroma-free hemoglobin (SFH). The intravascular halftime (T50) of the DBBF-SFH, determined from plasma hemoglobin levels, was 0.5 hours in the first 10 minutes and 4.3 hours during the next 50 minutes. At 24 hours, less than 5% of the DBBF-SFH remained. Elution of 51Cr was reflected in a lower T50 determined from the radioactivity levels: during the first 10 minutes the T50 was 0.3 hours; in the next 50 minutes it was 1 hour. The radioactivity sequestered in each organ in the first hour following DBBF-SFH infusion was as follows: 11.2% of the infused radioactivity was in the skin, 11.4% in muscle, 9.1% in the skeleton, and 5% in the liver. After 24 hours, the percentages in skin, muscle, skeleton and liver were 15.4, 10.3, 16.6 and 6.7% respectively. The percentage of infused radioactivity in the gastrointestinal tract and kidney at 1 hour and 24 hours ranged from 3.5 to 5.5%. Less than 0.4% was found in the spleen and lung. At 24 hours, 25% of the radioactivity was recovered in urine and 3% in feces.
Dittmer
J
J
Anatomy Department, Boston University School of Medicine, MA 02118.
Ichikura
T
T
Pivacek
L E
LE
Giorgio
A
A
Prusty
S
S
Valeri
C R
CR
D000818:Animals; D001241:Aspirin / Q000031:analogs & derivatives; D001802:Blood Substitutes / Q000302:isolation & purification / Q000493:pharmacokinetics*; D003432:Cross-Linking Reagents; D006207:Half-Life; D006454:Hemoglobins / Q000302:isolation & purification / Q000378:metabolism / Q000493:pharmacokinetics*; D006801:Humans; D008297:Male; D051379:Mice; D014018:Tissue Distribution
D001802:Blood Substitutes; D003432:Cross-Linking Reagents; D006454:Hemoglobins; C016484:hemoglobin, stroma free; C021630:bis(3,5-dibromosalicyl)fumarate; D001241:Aspirin