Development of analytical methods to evaluate SFH.
For the determination of stroma content in SFH, two analytical methods were developed, the HPLC-UV for phospholipids and the enzyme immunoassay for blood group antigen. These analytical methods were applied to evaluate three representative SFH preparations. The 36,000 x g centrifugation method was shown to contain higher amount of stroma components. On the other hand, the BMM-40 nm and the ultrafiltration method with a 100 kd membrane was shown to remove more than 99.7% of phospholipids and almost all of blood group antigen from hemoglobin solutions. These results demonstrated that these analytical methods were very useful in evaluating any highly purified hemoglobin solutions.
Nakai
K
K
Hokkaido Red Cross Blood Center, Sapporo, Japan.
Abe
H
H
Matsuda
N
N
Kobayashi
M
M
Ikeda
H
H
Sekiguchi
S
S
Tsuchida
E
E
D000017:ABO Blood-Group System / Q000032:analysis; D001802:Blood Substitutes / Q000032:analysis* / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005069:Evaluation Studies as Topic; D006454:Hemoglobins / Q000032:analysis* / Q000302:isolation & purification; D006801:Humans; D007124:Immunoenzyme Techniques; D010743:Phospholipids / Q000032:analysis; D011786:Quality Control; D012996:Solutions
D000017:ABO Blood-Group System; D001802:Blood Substitutes; D006454:Hemoglobins; D010743:Phospholipids; D012996:Solutions; C016484:hemoglobin, stroma free