title: Human interleukin-1 receptor antagonist. High yield expression in E. coli and examination of cysteine residues.
abstract: The human IL-1 receptor antagonist (IL-1ra) was produced in a high yield E. coli expression system, and was purified in a rapid two-step purification. This recombinant IL-1ra molecule possessed full binding activity to the IL-1 receptor (type I) and totally inhibited IL-1-induced PGE2 production by human dermal fibroblasts. Radioalkylation and analysis of V8-derived IL-1ra peptides indicate that the four cysteines present in the IL-1ra are not disulphide-linked.
authors.affiliation: Department of Biochemistry, University of Oxford, UK.
authors.forename: A
authors.initials: A
authors.lastname: Steinkasserer
authors.forename: R
authors.initials: R
authors.lastname: Solari
authors.forename: H R
authors.initials: HR
authors.lastname: Mott
authors.forename: R T
authors.initials: RT
authors.lastname: Aplin
authors.forename: C C
authors.initials: CC
authors.lastname: Robinson
authors.forename: A C
authors.initials: AC
authors.lastname: Willis
authors.forename: R B
authors.initials: RB
authors.lastname: Sim
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D001667:Binding, Competitive; D003001:Cloning, Molecular; D003545:Cysteine / Q000235:genetics*; D015232:Dinoprostone / Q000096:biosynthesis; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics*; D006801:Humans; D053590:Interleukin 1 Receptor Antagonist Protein; D007375:Interleukin-1 / Q000037:antagonists & inhibitors* / Q000378:metabolism; D008969:Molecular Sequence Data; D009841:Oligonucleotides; D011506:Proteins / Q000235:genetics*; D011971:Receptors, Immunologic / Q000378:metabolism; D017472:Receptors, Interleukin-1; D011994:Recombinant Proteins / Q000235:genetics; D012795:Sialoglycoproteins
chemical_list: C507763:IL1RN protein, human; D053590:Interleukin 1 Receptor Antagonist Protein; D007375:Interleukin-1; D009841:Oligonucleotides; D011506:Proteins; D011971:Receptors, Immunologic; D017472:Receptors, Interleukin-1; D011994:Recombinant Proteins; D012795:Sialoglycoproteins; D015232:Dinoprostone; D003545:Cysteine