title: Dog lymphocyte cultures facilitate the isolation and growth of virulent canine distemper virus.
abstract: Optimal conditions for the isolation and growth of virulent canine distemper virus (CDV) in canine thymic and peripheral blood lymphocyte cultures were determined. Peak virus titers were seen from 3 to 6 days postinoculation of lymphocytes and depended on the multiplicity of infection. Dog lymphocytes were at least as susceptible as canine macrophages to infection with virulent CDV. Virus replication in lymphocytes resulted in higher virus titers than in dog lung macrophages. Peripheral blood lymphocytes (PBL) from CDV-immune dogs were as susceptible to CDV as were PBL from susceptible dogs.
authors.affiliation: James A. Baker Institute for Animal Health, Department of Microbiology, Immunology and Parasitology, Ithaca, NY.
authors.forename: M J
authors.initials: MJ
authors.lastname: Appel
authors.forename: S
authors.initials: S
authors.lastname: Pearce-Kelling
authors.forename: B A
authors.initials: BA
authors.lastname: Summers
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004198:Disease Susceptibility; D004216:Distemper / Q000276:immunology / Q000382:microbiology*; D004217:Distemper Virus, Canine / Q000254:growth & development / Q000302:isolation & purification* / Q000472:pathogenicity; D004283:Dog Diseases / Q000276:immunology / Q000382:microbiology*; D004285:Dogs; D005289:Ferrets / Q000382:microbiology; D008214:Lymphocytes / Q000382:microbiology*; D008264:Macrophages / Q000382:microbiology; D008907:Mink / Q000382:microbiology; D011821:Raccoons / Q000382:microbiology; D013047:Specific Pathogen-Free Organisms; D013950:Thymus Gland / Q000166:cytology; D014774:Virulence; D014779:Virus Replication