title: GMP-140 (P-selectin) inhibits human neutrophil activation by lipopolysaccharide: analysis by proton magnetic resonance spectroscopy.
abstract: Proton magnetic resonance spectroscopy has been used to monitor the effect of GMP-140 on the stimulation of human peripheral blood neutrophils. Stimulation of neutrophils by lipopolysaccharide gives rise to a high resolution lipid spectrum from the intact cells. Fluid phase GMP-140, which prevents adhesion and development of inflammatory responses of neutrophils, was found to inhibit these changes in the lipid spectrum by up to 40%. Anti-GMP-140 Fab fragments reversed this effect while non-immune Fab fragments did not affect the observed inhibition by GMP-140.
authors.affiliation: Centre for Infectious Diseases and Microbiology, University of Sydney, Westmead Hospital, NSW, Australia.
authors.forename: G L
authors.initials: GL
authors.lastname: May
authors.forename: L C
authors.initials: LC
authors.lastname: Dunlop
authors.forename: K
authors.initials: K
authors.lastname: Sztelma
authors.forename: M C
authors.initials: MC
authors.lastname: Berndt
authors.forename: T C
authors.initials: TC
authors.lastname: Sorrell
mesh_terms: D015815:Cell Adhesion Molecules / Q000276:immunology / Q000378:metabolism*; D002455:Cell Division; D006801:Humans; D007140:Immunoglobulin Fab Fragments; D050356:Lipid Metabolism; D008070:Lipopolysaccharides / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D009504:Neutrophils / Q000187:drug effects / Q000502:physiology*; D019007:P-Selectin; D010980:Platelet Membrane Glycoproteins / Q000276:immunology / Q000378:metabolism*; D011522:Protons; D012486:Salmonella typhimurium
chemical_list: D015815:Cell Adhesion Molecules; D007140:Immunoglobulin Fab Fragments; D008070:Lipopolysaccharides; D019007:P-Selectin; D010980:Platelet Membrane Glycoproteins; D011522:Protons