Isolation of mouse Lyt2- mutant cells using magnetic particles.
A procedure is described for the selection of mouse Lyt2- mutant cells. The procedure is based upon repeated cycles of selection with rat monoclonal antibodies to the Lyt2 antigen and magnetic particles coated with goat anti-mouse IgG. Stable Lyt2- mutant clones were derived from cells previously mutagenized with X rays and, at a lower frequency, also from non-mutagenized cells. The procedure can be used to rescue Lyt2- cells when the ratio of Lyt2+:Lyt2- cells is 10(4):1.
Iannelli
D
D
School of Agriculture, University of Naples, Italy.
Capparelli
R
R
Scala
F
F
Ferrara
L
L
D000818:Animals; D000950:Antigens, Ly / Q000032:analysis / Q000235:genetics*; D002469:Cell Separation / Q000379:methods*; D007158:Immunologic Techniques; D066298:In Vitro Techniques; D008212:Lymphocyte Depletion; D008280:Magnetics; D051379:Mice; D009154:Mutation; D016176:T-Lymphocyte Subsets / Q000166:cytology*
D000950:Antigens, Ly