Sequence and functional analysis of the cloned Neisseria meningitidis CMP-NeuNAc synthetase.
The CMP-N-acetylneuraminic acid (CMP-NeuNAc) synthetase gene of Neisseria meningitidis group B is located on a 2.3-kb EcoRI fragment within the cps gene cluster. Nucleotide sequence determination of the gene encoding the CMP-NeuNAc synthetase revealed a 515-bp open reading frame that can encode a 18.9-kDA protein. A computer data base scan revealed a 59.4% identity to the CMP-NeuNAc synthetase gene of E. coli K1. Enzymatic activity was confirmed in vitro and in vivo. Transformation of the CMP-NeuNAc defective E. coli K1 strain EV5 with the meningococcal CMP-NeuNAc synthetase could complement the defect in E. coli.
Edwards
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U
Institut für Medizinische Mikrobiologie, Medizinische Hochschule Hannover, FRG.
Frosch
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M
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D000216:N-Acylneuraminate Cytidylyltransferase / Q000235:genetics*; D009345:Neisseria meningitidis / Q000201:enzymology* / Q000235:genetics*
D004269:DNA, Bacterial; D000216:N-Acylneuraminate Cytidylyltransferase