title: Staining of semithin tissue sections embedded in HPMA, quetol 523 and MMA.
abstract: Various tissues fixed in a mixture of formaldehyde and glutaraldehyde, and embedded in an improved 2-hydroxypropyl methacrylate mixture were employed for studying the fine structures of cells and tissues by light microscopy. The embedding mixture contained Quetol 523 and methyl methacrylate as a plasticizer without a cross-linker. The catalyst was QCU-1. The mixture had a low viscosity, was easy to handle and penetrated readily and completely into the specimen, producing a homogeneous block from which it was easy to cut sections of 1-2 microns in thickness. A wide variety of stains have been employed with such sections and those reported here are hematoxylin-eosin, Azan and PAS. There was excellent preservation of alkaline phosphatase activity. A method of poststaining immunoperoxidase labeling was also applied to the mouse pancreas and examples of staining with insulin are included.
authors.affiliation: Department of Anatomy, Tokyo Women's Medical College, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Iijima
authors.forename: Y
authors.initials: Y
authors.lastname: Nagato
authors.forename: T
authors.initials: T
authors.lastname: Kushida
mesh_terms: D010131:Aminosalicylic Acid; D004801:Eosine Yellowish-(YS); D006416:Hematoxylin; D008689:Methacrylates; D020366:Methylmethacrylate; D008768:Methylmethacrylates; D011102:Polyhydroxyethyl Methacrylate / Q000031:analogs & derivatives*; D013194:Staining and Labeling / Q000379:methods*; D016610:Tissue Embedding / Q000379:methods*
chemical_list: D008689:Methacrylates; D008768:Methylmethacrylates; C020689:methoxypolyethylene glycol 400 methacrylate; D020366:Methylmethacrylate; D011102:Polyhydroxyethyl Methacrylate; D010131:Aminosalicylic Acid; D004801:Eosine Yellowish-(YS); C032802:hydroxypropyl methacrylate; D006416:Hematoxylin