Production of hybrid bispecific antibody recognizing human colorectal carcinoma and CD3 antigen.
The present study reports on the use of gene transfer by vector DNA in the generation of hybrid hybridoma, the quadroma secreting the hybrid bispecific antibody. A quadroma B72.3neo/OKT3gpt was simply derived from the fusion of two hybridoma cell lines, B72.3 and OKT3, tagged with vector DNA mpSV2neo and mpSV2gpt, respectively, and selected in the media containing both G418 and mycophenolic acid. The hybrid bispecific antibody B72.3/OKT3 was purified from the quadroma ascites by the use of hydroxylapatite column on high-pressure liquid chromatography. This bispecific antibody contained one binding site for the TAG72 antigen on OVCAR3 tumor cells and the other binding site for the CD3 molecule on human T cells. It was able to target human T lymphocytes to significantly lyse the human ovarian cancer cells and may therefore be useful in immunotherapy of cancer.
Xiang
J
J
Saskatoon Cancer Center, Department of Microbiology, Saskatchewan, Canada.
Pan
Z
Z
Attah-Poku
S
S
Babiuk
L
L
Zhang
Y
Y
Liu
E
E
D000911:Antibodies, Monoclonal / Q000235:genetics; D000912:Antibodies, Neoplasm / Q000235:genetics; D000918:Antibody Specificity; D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology*; D017252:CD3 Complex; D015179:Colorectal Neoplasms / Q000276:immunology*; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D011948:Receptors, Antigen, T-Cell / Q000276:immunology*; D014162:Transfection
D000911:Antibodies, Monoclonal; D000912:Antibodies, Neoplasm; D000945:Antigens, Differentiation, T-Lymphocyte; D017252:CD3 Complex; D011948:Receptors, Antigen, T-Cell