Mononuclear cells in normal colon and colonic carcinoma express the same T cell activation markers.
The proportion of mononuclear cells (MC) expressing some of the T cell activation markers in normal colon and colonic carcinomas was determined by immunoperoxidase staining and computer-assisted video image analysis (VIA). Tissue sections were stained with monoclonal antibodies against the T cell activation markers which included the MHC class II antigens DR, DP and DQ, interleukin 2 receptor (CD25) and the p180 (CD45RO) form of the leucocyte common antigen. The mean values for the proportion of leucocytes expressing these activation markers were 92% for DR, 61% for DP, 68% for DQ and 60% for CD45RO in tumours and 90, 62, 70 and 55% in normal tissue respectively. Few cells were stained for the IL2 receptor (mean values 7% for tumours and 5% for normal tissue) or the p220 form (CD45RA) of the leucocyte common antigen (mean values 6% for tumours and 4% for normal tissue). The proportion of MC bearing any of these markers in the normal colon was not significantly different from the matched colonic carcinomas.
Norazmi
M N
MN
Department of Immunology, Flinders Medical Centre, Bedford Park, South Australia.
Hohmann
A W
AW
Skinner
J M
JM
Bradley
J
J
D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000032:analysis*; D015415:Biomarkers / Q000032:analysis; D002277:Carcinoma / Q000473:pathology*; D003106:Colon / Q000166:cytology*; D003110:Colonic Neoplasms / Q000473:pathology*; D006681:HLA-D Antigens / Q000032:analysis*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000737:chemistry*; D008213:Lymphocyte Activation
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D015415:Biomarkers; D006681:HLA-D Antigens