Semidry electrophoretic transfer of RNA to membranes.
We describe a method using a semi-dry gel electro-blotter to transfer RNA from standard agarose-formaldehyde denaturing gels in less than 30 min. The method requires equilibrating the gel in a low ionic strength buffer. The transfer is done under high-current and low-voltage conditions. This method maintains the overall sharpness of the bands on the final autoradiogram while shortening the time required for Northern transfer by approximately 12 hours.
Lane
K B
KB
Department of Pathology, Vanderbilt University School of Medicine, Nashville, TN 37232-2561.
Zoia
O
O
Davidson
J M
JM
D000818:Animals; D001709:Biotechnology; D002021:Buffers; D004549:Elastin / Q000235:genetics; D004587:Electrophoresis, Agar Gel / Q000295:instrumentation / Q000379:methods*; D005069:Evaluation Studies as Topic; D009131:Muscle, Smooth, Vascular / Q000378:metabolism; D012313:RNA / Q000235:genetics / Q000302:isolation & purification*; D013552:Swine
D002021:Buffers; D012313:RNA; D004549:Elastin