title: Soybean trypsin inhibitor and beta-amylase induce alveolar macrophages to release nitrogen oxides.
abstract: Rat alveolar macrophages incubated with soybean trypsin inhibitor and beta-amylase produced nitrite in a dose- and time-dependent manner. This production depends on the presence of L-arginine (L-arg) in the culture medium. The precursor of this nitrite was demonstrated as being nitric oxide by bleaching ferredoxin at 410 nm when added to the culture medium. NG-Monomethyl-L-arginine and the tetrahydrobiopterin biosynthesis inhibitor 2,4-diamino-6-hydroxypyrimidine inhibited the release of nitrite in a dose-dependent manner. Dexamethasone was able to modulate this release. These data indicate that alveolar macrophages are capable of secreting L-arg-derived nitrogen oxides when stimulated with certain alimentary proteins.
authors.affiliation: Department of Respiratory Medicine, University of Antwerp, Belgium.
authors.forename: P G
authors.initials: PG
authors.lastname: Jorens
authors.forename: F J
authors.initials: FJ
authors.lastname: Van Overveld
authors.forename: H
authors.initials: H
authors.lastname: Bult
authors.forename: P A
authors.initials: PA
authors.lastname: Vermeire
authors.forename: A G
authors.initials: AG
authors.lastname: Herman
mesh_terms: D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives / Q000494:pharmacology; D002478:Cells, Cultured / Q000187:drug effects; D003907:Dexamethasone / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D007042:Hypoxanthines / Q000494:pharmacology; D008033:Limulus Test; D016676:Macrophages, Alveolar / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D009573:Nitrites / Q000378:metabolism; D009589:Nitrogen Oxides / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors; D014361:Trypsin Inhibitors / Q000494:pharmacology*; D001614:beta-Amylase / Q000494:pharmacology*; D019323:omega-N-Methylarginine
chemical_list: D007042:Hypoxanthines; D009573:Nitrites; D009589:Nitrogen Oxides; D014361:Trypsin Inhibitors; D019323:omega-N-Methylarginine; D003907:Dexamethasone; D001120:Arginine; D001614:beta-Amylase; C017202:2,4-diaminohypoxanthine