Assignment of mutant tsN19 (complementation group E) of respiratory syncytial virus to the P protein gene.
The mutation responsible for the temperature-sensitive (ts) phenotype of mutant tsN19 (complementation group E) of respiratory syncytial virus has been located to the P protein gene. Viral protein synthesis was completely restricted at 39 degrees C, and the tsN19 P protein did not react with an anti-P monoclonal antibody (MAb) (3-5) at 33 degrees C. Reversion of temperature sensitivity restored reactivity with MAb 3-5. Nucleotide sequence determination and in vitro expression of cDNA clones of P mRNA derived from wild-type, tsN19 and non-ts revertant-infected cells, revealed that temperature sensitivity and loss of reactivity with MAb 3-5 were consequences of a Gly----Ser amino acid change at position 172. A low M(r) polypeptide, which represented the C-terminal 93 amino acids of the P protein, was produced by internal initiation in the P open reading frame during in vitro translation, and a similar product was detected transiently in vivo.
Caravokyri
C
C
Biological Sciences Department, University of Warwick, Coventry, U.K.
Zajac
A J
AJ
Pringle
C R
CR
D000818:Animals; D000911:Antibodies, Monoclonal; D000956:Antigens, Viral / Q000096:biosynthesis / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004277:DNA, Single-Stranded / Q000235:genetics; D000939:Epitopes / Q000235:genetics; D005814:Genes, Viral / Q000235:genetics*; D005816:Genetic Complementation Test; D015707:HN Protein; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics; D012136:Respiratory Syncytial Viruses / Q000235:genetics*; D014759:Viral Envelope Proteins; D014764:Viral Proteins
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D004277:DNA, Single-Stranded; D000939:Epitopes; D015707:HN Protein; D012333:RNA, Messenger; D014759:Viral Envelope Proteins; D014764:Viral Proteins; C116204:attachment protein G