A straight correlation between mutagenic activity and beta-galactosidase activity induced by monofunctional alkylating agents.
Eight monofunctional alkylating agents were examined for their ability to induce mutation in Salmonella typhimurium. The assay was carried out in S. typhimurium TA100 with the preincubation method. The SN1-type agents were more mutagenic than the SN2-type ones; besides, methylating agents exerted more mutagenic activity than ethylating ones. Those responses in the reversion assay were quite similar to the results obtained previously with the beta-galactosidase assay in Escherichia coli CSH26/pMCP1000 (alkA'-lacZ') as to the induction of the adaptive response. A good correlation was found between mutagenic potency in the reverse mutation assay and inducing potency in the beta-galactosidase assay.
Ohtsuka
M
M
Hita Research Laboratories, Chemicals Inspection and Testing Institute, Oita, Japan.
Maekawa
K
K
D000477:Alkylating Agents / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D016296:Mutagenesis; D009152:Mutagenicity Tests; D009153:Mutagens / Q000494:pharmacology*; D012486:Salmonella typhimurium / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics; D013329:Structure-Activity Relationship; D001616:beta-Galactosidase / Q000378:metabolism*
D000477:Alkylating Agents; D009153:Mutagens; D001616:beta-Galactosidase