title: Determination of erythromycin ethylsuccinate by liquid chromatography.
abstract: A method is described for the determination of erythromycin ethylsuccinate by liquid chromatography. A C18 reversed-phase column (25 cm x 4.6 mm I.D.) was used with acetonitrile-0.2 M tetrabutylammonium sulphate (pH 6.5)-0.2 M phosphate buffer (pH 6.5)-water [x:5:5:(90-x)] as mobile phase. The proportion of acetonitrile (x) has to be adapted to the type of stationary phase used. For RSil C18 LL, 42.5% was used. The column was heated at 35 degrees C, the flow-rate was 1.5 ml/min and UV detection was performed at 215 nm. The main component, erythromycin A ethylsuccinate, was separated from all other components which were present in commercial samples. The main impurities were erythromycin A and the ethylsuccinate esters of erythromycin B and C. The amide N-ethylsuccinyl-N-demethylerythromycin A was shown to be present in all the samples examined. The method was successfully applied to the analysis of specialities.
authors.affiliation: Katholieke Universiteit Leuven, Laboratorium voor Farmaceutische Chemie, Belgium.
authors.forename: T
authors.initials: T
authors.lastname: Cachet
authors.forename: P
authors.initials: P
authors.lastname: Lannoo
authors.forename: J
authors.initials: J
authors.lastname: Paesen
authors.forename: G
authors.initials: G
authors.lastname: Janssen
authors.forename: J
authors.initials: J
authors.lastname: Hoogmartens
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D015643:Erythromycin Ethylsuccinate / Q000032:analysis* / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D015394:Molecular Structure
chemical_list: D015643:Erythromycin Ethylsuccinate