title: Peptidylglycine alpha-amidating monooxygenase activity and TRH and CRF biosynthesis. Role of copper.
abstract: Carboxy-terminal amidation of biologically active peptides, an important characteristic of more than half of these substances, occurs during the maturation process of peptide precursors. It is catalyzed by peptidylglycine alpha-amidating monooxygenase (PAM), an enzyme that is copper-dependent. We show here that alterations of copper stores in cultured cells from different origins (pancreas and hypothalamus) affect the immunoreactivity of thyrotropin-releasing hormone (TRH) and corticotropin-releasing factor (CRF) (two alpha-amidated peptides). This suggests that copper can affect neuropeptide biosynthesis and may play a role in the endocrine or central nervous system function.
authors.affiliation: Laboratoire de Neuroendocrinologie Expérimentale, INSERM U 297 Faculté de Médecine Nord, Marseille, France.
authors.forename: P
authors.initials: P
authors.lastname: Giraud
authors.forename: J Y
authors.initials: JY
authors.lastname: Maltèse
authors.forename: F
authors.initials: F
authors.lastname: Boudouresque
authors.forename: P
authors.initials: P
authors.lastname: Salers
authors.forename: L
authors.initials: L
authors.lastname: Ouafik
authors.forename: M
authors.initials: M
authors.lastname: Renard
authors.forename: F
authors.initials: F
authors.lastname: Pelen
authors.forename: C
authors.initials: C
authors.lastname: Oliver
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002478:Cells, Cultured; D002614:Chelating Agents / Q000494:pharmacology; D003300:Copper / Q000494:pharmacology*; D003346:Corticotropin-Releasing Hormone / Q000096:biosynthesis* / Q000378:metabolism; D004050:Ditiocarb / Q000494:pharmacology; D005333:Fetus / Q000166:cytology; D007031:Hypothalamus / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D007328:Insulin / Q000378:metabolism; D007515:Islets of Langerhans / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D006899:Mixed Function Oxygenases / Q000378:metabolism*; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes; D051381:Rats; D013973:Thyrotropin-Releasing Hormone / Q000096:biosynthesis* / Q000378:metabolism
chemical_list: D002614:Chelating Agents; D007328:Insulin; D009097:Multienzyme Complexes; D013973:Thyrotropin-Releasing Hormone; D003300:Copper; D003346:Corticotropin-Releasing Hormone; D004050:Ditiocarb; D006899:Mixed Function Oxygenases; C035189:peptidylglycine monooxygenase