title: Inhibition of uptake of 1-methyl-4-phenylpyridinium ion and dopamine in striatal synaptosomes by tobacco smoke components.
abstract: To determine whether the attenuation of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced neurotoxicity by tobacco smoke exposure is caused by inhibition of the neuronal uptake of 4-phenylpyridinium ion (MPP+), various tobacco components and a smoke extract were tested for inhibitory activity in striatal synaptosomes. A dimethylsulfoxide extract of tobacco smoke filtrate was found to inhibit the uptake of MPP+ and dopamine. These results suggest that inhibition of the neuronal dopamine uptake mechanism may account for the protective effects of smoke exposure on MPTP-induced neurotoxicity.
authors.affiliation: Department of Pharmacology and Toxicology, University of Louisville, KY 40292.
authors.forename: L A
authors.initials: LA
authors.lastname: Carr
authors.forename: J K
authors.initials: JK
authors.lastname: Basham
authors.forename: B K
authors.initials: BK
authors.lastname: York
authors.forename: P P
authors.initials: PP
authors.lastname: Rowell
mesh_terms: D015655:1-Methyl-4-phenylpyridinium / Q000378:metabolism*; D000818:Animals; D003342:Corpus Striatum / Q000187:drug effects / Q000378:metabolism*; D003864:Depression, Chemical; D004298:Dopamine / Q000378:metabolism*; D006834:Hydrazines / Q000494:pharmacology; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009538:Nicotine / Q000494:pharmacology*; D011725:Pyridines / Q000494:pharmacology; D013574:Synaptosomes / Q000378:metabolism*; D014028:Tobacco Smoke Pollution / Q000009:adverse effects / Q000032:analysis*
chemical_list: D006834:Hydrazines; D011725:Pyridines; D014028:Tobacco Smoke Pollution; C029424:hydrazine; C051448:4-phenylpyridine; D009538:Nicotine; D015655:1-Methyl-4-phenylpyridinium; D004298:Dopamine