Incorporation of [3H]hypoxanthine by short-term cultured Theileria sergenti and its inhibition by drugs.
Red blood cells parasitized with Theileria sergenti were cultured in vitro for a short period in microplate wells. Addition of [3H]hypoxanthine to cultures enabled us to titrate the intraerythrocytic parasite growth because a significant amount of [3H]hypoxanthine was incorporated into the parasitized red blood cells (PRBC) in proportion to the number of PRBC. The incorporation of [3H]hypoxanthine was almost completed in an early phase of incubation. Bovine peripheral blood leukocytes incubated with lysate of PRBC did not incorporate [3H]hypoxanthine, indicating that contaminating leukocytes were not involved in the incorporation in the PRBC culture. The incorporation of [3H]hypoxanthine was decreased markedly by the addition of certain anti-parasitic drugs such as chloroquine, quinine, and pyrimethamine. This inhibition test was performed quantitatively with high sensitivity. Based on these results, this short-term culture seemed to be useful for drug screening or studying the mechanisms of theilerial infection. However, anti-T. sergenti antibodies failed to inhibit the [3H]hypoxanthine incorporation.
Kamiyama
T
T
Department of Veterinary Science, National Institute of Health, Tokyo, Japan.
Niinuma
S
S
Ikeda
I
I
Matsubara
J
J
D000818:Animals; D000913:Antibodies, Protozoan / Q000276:immunology; D000981:Antiprotozoal Agents / Q000494:pharmacology*; D002417:Cattle; D002478:Cells, Cultured; D002738:Chloroquine / Q000494:pharmacology; D004133:Diminazene / Q000031:analogs & derivatives / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004912:Erythrocytes / Q000469:parasitology*; D019271:Hypoxanthine; D007042:Hypoxanthines / Q000378:metabolism*; D007106:Immune Sera / Q000276:immunology; D011739:Pyrimethamine / Q000494:pharmacology; D011803:Quinine / Q000494:pharmacology; D016794:Theileria / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*
D000913:Antibodies, Protozoan; D000981:Antiprotozoal Agents; D007042:Hypoxanthines; D007106:Immune Sera; D019271:Hypoxanthine; D002738:Chloroquine; D011803:Quinine; C003915:diminazene aceturate; D004133:Diminazene; D011739:Pyrimethamine