Heterogeneous expression of blood group A-determinant in a human gastric cancer cell line derived from a blood group A individual.
The human gastric cancer cell line MKN 45 was derived from the tumour of a blood group A individual, and was known to express large quantities of blood group A-antigen. Using immunofluorescence we found the MKN 45 cells, donated from the Japanese Cancer Research Resources Bank, consisted of A-antigen positive cells (18%) and A-antigen negative cells (82%). After limiting dilution, wild type and mutant cells were cloned with regard to the expression of a cell surface A-antigen. ELISA was used to detect A-antigen in the cell extract of the wild type cells, but none was evident in those of the mutant cells. However, blood group A-gene-specified transferase activity of the mutant cells was comparable to that of the wild type cells.
Kominato
Y
Y
Department of Legal Medicine, Toyama Medical & Pharmaceutical University, Japan.
Fujikura
T
T
Takizawa
H
H
Hayashi
K
K
Fujimaki
M
M
Kishi
K
K
Suzuki
T
T
D000017:ABO Blood-Group System / Q000096:biosynthesis* / Q000235:genetics; D000939:Epitopes / Q000096:biosynthesis / Q000235:genetics; D005455:Fluorescent Antibody Technique; D015870:Gene Expression; D016695:Glycosyltransferases / Q000235:genetics; D006801:Humans; D011110:Polymorphism, Genetic; D013274:Stomach Neoplasms / Q000235:genetics / Q000276:immunology*; D014407:Tumor Cells, Cultured / Q000201:enzymology / Q000276:immunology
D000017:ABO Blood-Group System; D000939:Epitopes; D016695:Glycosyltransferases