title: A new crystal form of the complex between seryl-tRNA synthetase and tRNA(Ser) from Thermus thermophilus that diffracts to 2.8 A resolution.
abstract: Two distinct complexes between seryl-tRNA synthetase and tRNA(Ser) from Thermus thermophilus have been crystallized using ammonium sulphate as a precipitant. Form III crystals grow from solutions containing a 1:2.5 stoichiometry of synthetase dimer to tRNA. They are of monoclinic space group C2 with unit cell dimensions a = 211.6 A, b = 126.8 A, c = 197.1 A, beta = 132.4 degrees and diffract to about 3.5 A. Preliminary crystallographic results show that the crystallographic asymmetric unit contains two synthetase dimers. Form IV crystals grow from solutions containing a 1:1.5 stoichiometry of synthetase dimer to tRNA. They are of orthorhombic space group P2(1)2(1)2(1) with unit cell dimensions a = 124.5 A, b = 128.9 A, c = 121.2 A and diffract to 2.8 A resolution. Preliminary crystallographic results show that these crystals contain only one tRNA molecule bound to a synthetase dimer.
authors.affiliation: Institute of Molecular Biology and Genetics, Academy of Sciences of the Ukraine, Kiev.
authors.forename: A D
authors.initials: AD
authors.lastname: Yaremchuk
authors.forename: M A
authors.initials: MA
authors.lastname: Tukalo
authors.forename: I
authors.initials: I
authors.lastname: Krikliviy
authors.forename: N
authors.initials: N
authors.lastname: Malchenko
authors.forename: V
authors.initials: V
authors.lastname: Biou
authors.forename: C
authors.initials: C
authors.lastname: Berthet-Colominas
authors.forename: S
authors.initials: S
authors.lastname: Cusack
mesh_terms: D003460:Crystallization; D004591:Electrophoresis, Polyacrylamide Gel; D012362:RNA, Transfer, Ser / Q000737:chemistry* / Q000378:metabolism; D012714:Serine-tRNA Ligase / Q000737:chemistry* / Q000378:metabolism; D016963:Thermus thermophilus / Q000737:chemistry* / Q000201:enzymology; D014961:X-Ray Diffraction
chemical_list: D012362:RNA, Transfer, Ser; D012714:Serine-tRNA Ligase