A simple procedure for recovering the denaturing effect of methylmercury in agarose gel electrophoresis.
A simple and rapid procedure for recovering the denaturing effect of methylmercuric hydroxide in agarose gel electrophoresis is described. The procedure consisted of the treatment of the commercial methylmercuric hydroxide solutions with Amberlite, a mixture of anion- and cation-exchange resins. This treatment greatly improved the resolution of RNA species when fractionated by electrophoresis through agarose-CH3HgOH slab gel.
Fernández-Silva
P
P
Departamento de Bioquímica y Biología Molecular y Celular, Facultad de Veterinaria, Zaragoza, Spain.
Enriquez
J A
JA
Montoya
J
J
D000818:Animals; D004587:Electrophoresis, Agar Gel / Q000379:methods*; D008767:Methylmercury Compounds / Q000009:adverse effects*; D009691:Nucleic Acid Denaturation / Q000187:drug effects*; D012313:RNA; D051381:Rats; D012117:Resins, Synthetic
D008767:Methylmercury Compounds; D012117:Resins, Synthetic; D012313:RNA; C046797:amberlite; C020771:methylmercury hydroxide