title: Effect of 5,7-unsaturated sterols on ethanol tolerance in Saccharomyces cerevisiae.
abstract: Structural membrane lipids are known to contribute to the high ethanol resistance of Saccharomyces cerevisiae (2, 4, 17). By manipulating the yeast cellular sterol level by changing the carbon-to-nitrogen source ratio in the chemostat growth medium, high delta 5,7-sterol levels were found to increase the resistance of yeast populations to ethanol-induced death. The resistance of the erg2 (delta 8----delta 7-sterol isomerase) mutant to ethanol-induced death was generally comparable with that of the delta 5,7-sterol-synthesizing strain. In contrast, the sensitivity of anaerobic growth to inhibition by ethanol was higher in the erg2 mutant in comparison with the delta 5,7-sterol-synthesizing strains but a high level of those sterols increased the vulnerability of anaerobic growth to ethanol inhibition.
authors.affiliation: Czechoslovak Academy of Sciences, Prague.
authors.forename: C
authors.initials: C
authors.lastname: Novotný
authors.forename: M
authors.initials: M
authors.lastname: Flieger
authors.forename: J
authors.initials: J
authors.lastname: Panos
authors.forename: F
authors.initials: F
authors.lastname: Karst
mesh_terms: D000693:Anaerobiosis; D000431:Ethanol / Q000494:pharmacology*; D005227:Fatty Acids / Q000378:metabolism; D005800:Genes, Fungal; D009154:Mutation; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D013251:Steroid Isomerases / Q000235:genetics / Q000378:metabolism; D013261:Sterols / Q000378:metabolism / Q000494:pharmacology*
chemical_list: D005227:Fatty Acids; D013261:Sterols; D000431:Ethanol; D013251:Steroid Isomerases; C022669:delta(8)-delta(7)-sterol isomerase