title: Among naive precursor cell subpopulations only progenitors of memory B cells originate germinal centers.
abstract: Immunization leads to the generation of both antibody-forming cells (AFC) and memory B cells which are thought to arise in germinal centers within lymphoid follicles. The findings that the precursors to memory B cells reside in the J11Dlo subpopulation of the spleens in non-immune mice and that this subpopulation is distinct from conventional AFC precursors, including CD5+ B cells, suggest that the precursors of germinal centers might also reside in the J11Dlo subpopulation. To test this hypothesis, SCID mice were repopulated with CD4+ carrier-primed T cells and T-depleted J11Dlo, J11Dhi or CD5+ B cells and immunized with a hapten-carrier conjugate. Only the J11Dlo population was enriched for cells that produced germinal centers. Thus, the subpopulation of precursors that generates memory B cells also originates germinal centers.
authors.affiliation: Department of Immunology, Scripps Research Institute, La Jolla, CA.
authors.forename: P J
authors.initials: PJ
authors.lastname: Linton
authors.forename: D
authors.initials: D
authors.lastname: Lo
authors.forename: L
authors.initials: L
authors.lastname: Lai
authors.forename: G J
authors.initials: GJ
authors.lastname: Thorbecke
authors.forename: N R
authors.initials: NR
authors.lastname: Klinman
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000032:analysis; D001402:B-Lymphocytes / Q000276:immunology*; D018956:CD5 Antigens; D006412:Hematopoietic Stem Cells / Q000276:immunology*; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D007156:Immunologic Memory; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D016513:Mice, SCID
chemical_list: D015703:Antigens, CD; D018956:CD5 Antigens; D000949:Histocompatibility Antigens Class II