Native epitopes of human IgA.
We have studied the epitopes of the human IgA molecule by using 27 monoclonal antibodies in two simple gel diffusion methods. By testing pairs of monoclonal antibodies for coprecipitation of an IgA myeloma protein, we have clearly identified sterically independent epitopes on the molecule. By testing the non-coprecipitating monoclonal antibodies with soluble IgA-monoclonal antibody immune complexes, we have identified the overlapping epitopes. Eleven proto-epitopes were mapped on the IgA molecule. Since we have not used solid-phase methods, we have presumably identified native epitopes of the IgA molecule.
Molinaro
G A
GA
Department of Microbiology, Loma Linda University, CA 92350.
Bui
J D
JD
Eby
W C
WC
D000911:Antibodies, Monoclonal / Q000276:immunology; D000918:Antibody Specificity; D000936:Antigen-Antibody Complex / Q000276:immunology; D000939:Epitopes / Q000276:immunology*; D006801:Humans; D005779:Immunodiffusion; D007070:Immunoglobulin A / Q000276:immunology*; D007145:Immunoglobulin kappa-Chains / Q000276:immunology; D009194:Myeloma Proteins / Q000276:immunology; D011233:Precipitin Tests
D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D000939:Epitopes; D007070:Immunoglobulin A; D007145:Immunoglobulin kappa-Chains; D009194:Myeloma Proteins