Variation in the antigenic characteristics of venom from the Mojave rattlesnake (Crotalus scutulatus scutulatus).
Venoms from 31 specimens of the Mojave rattlesnake (Crotalus scutulatus scutulatus) were examined to further characterize reported differences among venoms of this species. Twenty-two venoms were recognized by a monoclonal antibody to Mojave toxin, CSS12. Nine venoms were recognized by CA-P-8, a monoclonal antibody produced against the hemorrhagic venom of C. atrox. Seven of these produced strong hemorrhage in mice and were also recognized by polyclonal antibodies (anti-F5) produced against a fraction of Mojave rattlesnake venom that inactivates serum complement. Fractionated venom revealed that CA-P-8 and anti-F5 recognized different proteins. Two of the venoms recognized by CA-P-8 were not recognized by anti-F5 and produced minimal hemorrhage in mice. This suggests that more than one factor may be necessary to induce strong hemorrhage.
Huang
S Y
SY
Department of Biology, Texas A&I University, Kingsville 78363.
Perez
J C
JC
Rael
E D
ED
Lieb
C
C
Martinez
M
M
Smith
S A
SA
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000940:Antigenic Variation; D002851:Chromatography, High Pressure Liquid; D003435:Crotalid Venoms / Q000276:immunology* / Q000633:toxicity; D000939:Epitopes / Q000032:analysis*; D006470:Hemorrhage / Q000139:chemically induced; D015151:Immunoblotting; D007525:Isoelectric Focusing; D051379:Mice; D008807:Mice, Inbred BALB C; D012911:Snakes
D000911:Antibodies, Monoclonal; D003435:Crotalid Venoms; D000939:Epitopes