Structure and chromosomal localization of the human gene for a brain form of prostaglandin D2 synthase.
We have cloned and characterized the human gene for the 21-kDa brain form of prostaglandin D2 synthase. The gene was isolated from a human genomic lambda library and spans 3600 base pairs. It consists of seven exons and six introns. Southern blot analysis indicates that there is a single copy of the gene in the haploid genome. The transcriptional start site was mapped to a G residue 74 base pairs 5' of the ATG initiation codon. A TATA box-like element (ATAAATA) is situated 21 base pairs upstream of the mRNA start site. The gene was mapped to chromosome 9 bands q34.2-q34.3. The gene bears close resemblance to the genes for murine major urinary protein and ovine beta-lactoglobulin.
White
D M
DM
Department of Biochemistry and Molecular Biology, University of Chicago, Illinois 60637.
Mikol
D D
DD
Espinosa
R
R
Weimer
B
B
Le Beau
M M
MM
Stefansson
K
K
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D001921:Brain / Q000201:enzymology*; D002871:Chromosome Banding; D002874:Chromosome Mapping; D002899:Chromosomes, Human, Pair 9; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D005091:Exons; D005333:Fetus; D006801:Humans; D019746:Intramolecular Oxidoreductases; D007527:Isoenzymes / Q000235:genetics*; D007535:Isomerases / Q000235:genetics*; D007782:Lactoglobulins / Q000235:genetics; D054834:Lipocalins; D051379:Mice; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D011506:Proteins / Q000235:genetics; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D017386:Sequence Homology, Amino Acid; D012756:Sheep; D013116:Spinal Cord / Q000201:enzymology; D016385:TATA Box
D007527:Isoenzymes; D007782:Lactoglobulins; D054834:Lipocalins; D009838:Oligodeoxyribonucleotides; D011506:Proteins; C035737:major urinary proteins; D012313:RNA; D004247:DNA; D007535:Isomerases; D019746:Intramolecular Oxidoreductases; C022466:prostaglandin R2 D-isomerase