Effects of glutathione on the synthesis and turnover of interleukin-2 receptors.
Internalization of IL-2 is important for its biological activities. The internalization of IL-2 was regulated by the duration of glutathione (GSH) treatment in CTLL-2 and CT-4R cells. Flow cytometric studies showed that the level of surface IL-2 receptors was not increased by GSH treatment. Northern blot analysis showed that the mRNA of IL-2Rp55 and IL-2Rp70, the two major components of the high-affinity IL-2 receptors, was increased 6 hr after GSH treatment. The appearance rate of membrane IL-2 receptors in GSH-treated cells was faster than that of the untreated cells. GSH also shortened the half-life (from 5 to less than or equal to 3 hr) and thus increased the turnover of the surface high-affinity IL-2 receptors. These results suggest that although GSH does not affect the level of surface IL-2 receptors, GSH may regulate the internalization of IL-2 by enhancing the synthesis and turnover of surface IL-2 receptors.
Liang
S M
SM
Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland 20892.
Lee
N
N
Chen
Y Y
YY
Liang
C M
CM
D000818:Animals; D002460:Cell Line; D004705:Endocytosis; D005978:Glutathione / Q000494:pharmacology*; D007376:Interleukin-2 / Q000378:metabolism; D051379:Mice; D012333:RNA, Messenger / Q000096:biosynthesis; D015375:Receptors, Interleukin-2 / Q000187:drug effects* / Q000378:metabolism
D007376:Interleukin-2; D012333:RNA, Messenger; D015375:Receptors, Interleukin-2; D005978:Glutathione