title: Biosynthesis of cholesterol linoleate by polyethylene glycol-modified cholesterol esterase in organic solvents.
abstract: Cholesterol esterase modified with polyethylene glycol was able to dissolve in some highly hydrophobic solvents such as benzene and toluene, and catalyze the synthesis of cholesterol linoleate with time dependency in the reverse of the usual reaction in aqueous solvents. Enzymatic cholesterol linoleate synthesis followed Michaelis-Menten kinetics which depended on the concentration of cholesterol and linoleic acid. When more than 20 mM of both substrates was used, the specific activity in this esterification was 200-250 nmol/min/mg protein. The apparent Km value for cholesterol and linoleic acid was 3.7 and 7.6 mM, respectively. The possibility of using such a modified enzyme for the synthesis of less stable cholesterol esters is discussed.
authors.affiliation: School of Health Sciences, Okayama University, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Mori
authors.forename: Y
authors.initials: Y
authors.lastname: Nakata
authors.forename: H
authors.initials: H
authors.lastname: Endo
mesh_terms: D002788:Cholesterol Esters / Q000096:biosynthesis*; D007700:Kinetics; D011092:Polyethylene Glycols; D011549:Pseudomonas / Q000201:enzymology; D012997:Solvents; D002787:Sterol Esterase / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D002788:Cholesterol Esters; D012997:Solvents; D011092:Polyethylene Glycols; C021976:cholesteryl linoleate; D002787:Sterol Esterase