A new crystal form of the complex between seryl-tRNA synthetase and tRNA(Ser) from Thermus thermophilus that diffracts to 2.8 A resolution.
Two distinct complexes between seryl-tRNA synthetase and tRNA(Ser) from Thermus thermophilus have been crystallized using ammonium sulphate as a precipitant. Form III crystals grow from solutions containing a 1:2.5 stoichiometry of synthetase dimer to tRNA. They are of monoclinic space group C2 with unit cell dimensions a = 211.6 A, b = 126.8 A, c = 197.1 A, beta = 132.4 degrees and diffract to about 3.5 A. Preliminary crystallographic results show that the crystallographic asymmetric unit contains two synthetase dimers. Form IV crystals grow from solutions containing a 1:1.5 stoichiometry of synthetase dimer to tRNA. They are of orthorhombic space group P2(1)2(1)2(1) with unit cell dimensions a = 124.5 A, b = 128.9 A, c = 121.2 A and diffract to 2.8 A resolution. Preliminary crystallographic results show that these crystals contain only one tRNA molecule bound to a synthetase dimer.
Yaremchuk
A D
AD
Institute of Molecular Biology and Genetics, Academy of Sciences of the Ukraine, Kiev.
Tukalo
M A
MA
Krikliviy
I
I
Malchenko
N
N
Biou
V
V
Berthet-Colominas
C
C
Cusack
S
S
D003460:Crystallization; D004591:Electrophoresis, Polyacrylamide Gel; D012362:RNA, Transfer, Ser / Q000737:chemistry* / Q000378:metabolism; D012714:Serine-tRNA Ligase / Q000737:chemistry* / Q000378:metabolism; D016963:Thermus thermophilus / Q000737:chemistry* / Q000201:enzymology; D014961:X-Ray Diffraction
D012362:RNA, Transfer, Ser; D012714:Serine-tRNA Ligase