L-selectin expression differentiates T cells isolated from different lymphoid tissues in cattle but does not correlate with memory.
L-selectin (LECAM-1, LAM-1) was expressed by a high proportion of CD4+ and CD8+ T cells, as well as almost all of the gamma delta T-cell receptor (TcR)+ (WC1+) T cells, isolated from blood, lymph nodes or tonsils. CD4+ T cells in the lamina propria of the gut villi and CD8+ T cells in the villous epithelium as well as the majority of WC1+ T cells in the gut mucosa were L-selectin-. The proportion of T cells from Peyer's patches that synthesized the molecule was intermediate between the value for blood and gut mucosa. Expression of L-selectin therefore marks T cells in cattle with a distinct tissue distribution that correlates with its function as the peripheral node homing receptor. The proportion of CD4+ and CD8+ T cells in the circulation that were L-selectin+ decreased with age. Unlike CD45R, expression of L-selectin was not related to CD4 T-cell memory as judged by proliferation in transformation assays to soluble antigen. Three-colour immunofluorescent staining demonstrated four subpopulations of CD4 and CD8 T lymphocytes in peripheral blood mononuclear cells (PBMC) that were CD45R+, L-selectin+; CD45R+, L-selectin-; CD45R-, L-selectin+; CD45R-, L-selectin-. CD4(4) memory cells were CD45R- and L-selectin+ or L-selectin-. Taken with earlier studies the reported observations demonstrated that only one of the four phenotypes of the CD4+ T cells in blood is present in the lamina propria of the gut villi and these are CD45R-, L-selectin-. Two of the four phenotypes of CD8+ T cells were present in the gut epithelium; these were CD45R+, L-selectin- or CD45R-, L-selectin-. Expression of the bovine molecule was not rapidly down-regulated on T cells following activation by exposure to phorbol myristate acetate.
Howard
C J
CJ
AFRC Institute for Animal Health, Compton, Berkshire, U.K.
Sopp
P
P
Parsons
K R
KR
D000375:Aging / Q000276:immunology; D000818:Animals; D015703:Antigens, CD / Q000032:analysis*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D002417:Cattle / Q000276:immunology*; D002478:Cells, Cultured; D007156:Immunologic Memory; D007413:Intestinal Mucosa / Q000276:immunology; D017493:Leukocyte Common Antigens / Q000032:analysis; D007962:Leukocytes / Q000276:immunology; D008213:Lymphocyte Activation / Q000276:immunology; D008221:Lymphoid Tissue / Q000276:immunology*; D010047:Ovalbumin / Q000276:immunology; D019007:P-Selectin; D010980:Platelet Membrane Glycoproteins / Q000032:analysis*; D013601:T-Lymphocytes / Q000276:immunology*
D015703:Antigens, CD; D019007:P-Selectin; D010980:Platelet Membrane Glycoproteins; D010047:Ovalbumin; D017493:Leukocyte Common Antigens