Purification and analysis of glycoproteins bearing blood group-A determinants from hamster pancreatic ductal adenocarcinomas.
Although the hamster pancreas does not express A, B or H blood group antigens, all hamster pancreatic ductal adenocarcinomas induced by treatment with N-nitrosobis(2-oxopropyl)amine express blood group-A antigen. Thus, the acquisition of blood group-A antigen expression in this system is a cancer-associated alteration. We have purified three major blood group-A antigen bearing glycoproteins (gp120, gp135 and gp150) from hamster pancreatic cancer cell membrane preparations using affinity chromatography on DBA (Dolichos biflorus) agglutinin-agarose. When assayed by immunoblotting, gp120 and gp135 showed strong blood group-A reactivity, which was removed by treating membrane samples with peptide-N-glycosidase F. Blood group-A reactivity was unchanged by treatment of the membrane fractions with endoglycosidases F and H. In addition, these two glycoproteins bearing blood group-A antigen also bound L-PHA (Phaseolus vulgaris leucoagglutinin). These results demonstrate that gp120 and gp135 express blood group-A antigen on Asn-linked multi-antennary complex type glycan structures. The gp150 showed weak blood group-A expression. This is the first demonstration of the neoexpression of cancer-associated blood group-A determinants which reside on Asn-linked glycan structures.
Hirota
M
M
Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha 68198-4525.
Pour
P M
PM
Tempero
M A
MA
Chaney
W G
WG
D000017:ABO Blood-Group System / Q000032:analysis / Q000276:immunology*; D000818:Animals; D000951:Antigens, Neoplasm / Q000032:analysis / Q000302:isolation & purification*; D002273:Carcinogens; D002285:Carcinoma, Intraductal, Noninfiltrating / Q000097:blood* / Q000139:chemically induced / Q000737:chemistry; D006224:Cricetinae; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000032:analysis; D006026:Glycoside Hydrolases / Q000378:metabolism; D015151:Immunoblotting; D037102:Lectins; D008562:Membrane Glycoproteins / Q000032:analysis / Q000302:isolation & purification*; D009602:Nitrosamines; D009844:Oligosaccharides / Q000032:analysis / Q000302:isolation & purification; D010179:Pancreas / Q000737:chemistry / Q000276:immunology; D010190:Pancreatic Neoplasms / Q000097:blood* / Q000139:chemically induced / Q000737:chemistry; D037121:Plant Lectins; D012967:Sodium Dodecyl Sulfate; D014407:Tumor Cells, Cultured
D000017:ABO Blood-Group System; D000951:Antigens, Neoplasm; D002273:Carcinogens; D000939:Epitopes; D037102:Lectins; D008562:Membrane Glycoproteins; D009602:Nitrosamines; D009844:Oligosaccharides; D037121:Plant Lectins; C036554:dolichos biflorus agglutinin; D012967:Sodium Dodecyl Sulfate; C013622:nitrosobis(2-oxopropyl)amine; D006026:Glycoside Hydrolases