title: Expression of renal organic cation transporter in Xenopus laevis oocytes.
abstract: The expression of the organic cation transport system of rat renal proximal tubules has been studied in Xenopus laevis oocytes injected with poly(A)+ RNA from the rat renal cortex. The effectiveness of the technique was confirmed by examining expression of the Na+/D-glucose co-transporter. Compared with water-injected and non-injected oocytes, the injection of total poly(A)+ RNA resulted in about a 3-fold increase in tetraethylammonium (TEA) uptake activity. TEA uptake by poly(A)(+)-RNA-injected oocytes was time-dependent and was inhibited by cimetidine and HgCl2, but not by p-aminohippurate. After size-fractionation on a sucrose density gradient, a 1.4-2.4 kb poly(A)+ RNA fragment was identified that expressed the organic cation transport system in oocytes. These results demonstrate that the renal organic cation transporter was expressed in oocytes and that this expression system can provide an effective assay procedure for cloning of the organic cation transporter.
authors.affiliation: Department of Pharmacy, Kyoto University Hospital, Faculty of Medicine, Japan.
authors.forename: R
authors.initials: R
authors.lastname: Hori
authors.forename: M
authors.initials: M
authors.lastname: Hirai
authors.forename: T
authors.initials: T
authors.lastname: Katsura
authors.forename: M
authors.initials: M
authors.lastname: Takano
authors.forename: M
authors.initials: M
authors.lastname: Yasuhara
authors.forename: S
authors.initials: S
authors.lastname: Kaneko
authors.forename: M
authors.initials: M
authors.lastname: Satoh
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D002927:Cimetidine / Q000494:pharmacology; D015870:Gene Expression; D007668:Kidney / Q000502:physiology*; D007672:Kidney Cortex / Q000502:physiology; D007679:Kidney Medulla / Q000502:physiology; D007687:Kidney Tubules, Proximal / Q000502:physiology; D008297:Male; D008627:Mercuric Chloride / Q000494:pharmacology; D009865:Oocytes / Q000187:drug effects / Q000502:physiology*; D011061:Poly A / Q000235:genetics* / Q000302:isolation & purification; D012313:RNA / Q000235:genetics* / Q000302:isolation & purification; D012333:RNA, Messenger; D051381:Rats; D011919:Rats, Inbred Strains; D019789:Tetraethylammonium; D013757:Tetraethylammonium Compounds / Q000378:metabolism; D014982:Xenopus laevis; D010130:p-Aminohippuric Acid / Q000494:pharmacology
chemical_list: D002352:Carrier Proteins; D012333:RNA, Messenger; D013757:Tetraethylammonium Compounds; D011061:Poly A; D008627:Mercuric Chloride; D012313:RNA; D019789:Tetraethylammonium; D002927:Cimetidine; D010130:p-Aminohippuric Acid