[Chemical-enzymatic synthesis and cloning in Escherichia coli of a gene coding for human granulocyte-colony stimulating factor].
Two artificial genes, encoding two forms of human granulocyte colony stimulating factor as products of a normal and an alternative splicing, have been by a chemical-enzymatic way synthesized and cloned in Escherichia coli. The genes are supplied with recognition sites of restriction endonucleases to facilitate the further cassette mutagenesis.
Kash'ian
S K
SK
Bystrov
N S
NS
Boldyreva
E F
EF
Poliakova
I A
IA
Severtsova
I V
IV
Korobko
V G
VG
D000595:Amino Acid Sequence; D001345:Autoradiography; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA; D004262:DNA Restriction Enzymes; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D005813:Genes, Synthetic; D016179:Granulocyte Colony-Stimulating Factor / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D010957:Plasmids; D012326:RNA Splicing
D016179:Granulocyte Colony-Stimulating Factor; D004247:DNA; D004262:DNA Restriction Enzymes