[An improvement of liver egg-immunoenzymatic staining technique for diagnosis of schistosomiasis japonica].
Using PVC membrane instead of common slide in liver egg-immunoenzymatic staining technique (IEST) for diagnosis of schistosomiasis japonica to detect specific antibodies in sera from 179 cases with schistosomiasis japonica, the positive rate was 97.2% (174/179). Sera from 63 healthy individuals revealed in one positive (1.5%). No cross reaction was observed in sera from 6 cases of paragonimiasis. PVC-IEST and SLIDE-IEST were comparatively performed on sera from 78 cases with schistosomiasis japonica, the positive rates were 96.2% (75/78) and 97.4% (76/78), respectively (P greater than 0.05). As PVC-IEST is simpler to perform than SLIDE-IEST, it is suggested that the former is more suitable for application in the field for diagnostic purposes.
Gu
Q Z
QZ
Hangzhou Branch, Zhejiang Medical University.
He
J G
JG
Chen
H
H
D000818:Animals; D006801:Humans; D007124:Immunoenzyme Techniques; D010063:Ovum / Q000276:immunology; D012549:Schistosoma japonicum / Q000276:immunology; D012554:Schistosomiasis japonica / Q000175:diagnosis*; D013194:Staining and Labeling / Q000379:methods