Characterization of the tRNA(Trp) genes of Saccharomyces cerevisiae.
The purpose of this work was to examine the tRNA(Trp)-encoding genes (tRNA(Trp)) of Saccharomyces cerevisiae to gain insight as to why tRNA(Trp) amber suppressors, isolated by conventional genetic techniques, have not been reported. The results herein indicate that the haploid yeast genome contains six tRNA(Trp) genes which map to five or six chromosomes. Not only do the six genes have identical coding sequences but their introns are also identical. Gene replacement experiments indicate that five copies of tRNA(Trp) are sufficient for cell viability. Thus, mutation of one tRNA(Trp) gene to a suppressor in vivo, lowering the functional number of tRNA(Trp) genes, would not be expected to be lethal.
Atkin
A L
AL
Department of Genetics, University of Alberta, Edmonton, Canada.
Henry
R W
RW
Roy
K L
KL
Bell
J B
JB
D001483:Base Sequence; D015139:Blotting, Southern; D002874:Chromosome Mapping; D015825:Chromosomes, Fungal; D003001:Cloning, Molecular; D004271:DNA, Fungal; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D012364:RNA, Transfer, Trp / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics*
D004271:DNA, Fungal; D012364:RNA, Transfer, Trp