[Studies on whole chromosomal DNA probes from Mycobacterium tuberculosis and Mycobacterium intracellulare].
The whole chromosomal DNA probes prepared from M. tuberculosis and M. intracellulare were used to hybridize in DNA dot blot and bacterial dot blot hybridization with 9 reference strains of Mycobacteria, including M. tuberculosis, M. bovis, M. avium, M. intracellulare, M kansasii, etc. These two probes showed good sensitivity and specificity for M. tuberculosis complex and M. avium complex respectively. Using the colony hybridization in situ, we can detect and identify M. tuberculosis with the probes on NC membrane after 1-2 week incubation over 7H9 agar plate.
Pan
Y X
YX
Beijing Institute of Tuberculosis.
D015139:Blotting, Southern / Q000379:methods; D015342:DNA Probes; D004269:DNA, Bacterial / Q000235:genetics; D015269:Mycobacterium avium Complex / Q000235:genetics*; D009169:Mycobacterium tuberculosis / Q000235:genetics* / Q000302:isolation & purification; D009693:Nucleic Acid Hybridization
D015342:DNA Probes; D004269:DNA, Bacterial