Improved microscopic detection of bacteriuria.
A simple method is described which permits the microscopic detection of bacteria in sediments of urine and other fluids, including bacteria that have eluded detection by conventional means. The method introduces increased centrifugal force and stepwise chemical fixation and then conventional staining. It is rapid, economical, and suitable for use in a physician's office. Use of this method immediately reveals those bacteria reported as "significant" by the conventional laboratory culture. More importantly, living or dead, which are missed by the conventional culture and by the conventional Gram staining procedure. These bacteria usually can be grown in special media and they appear to be related to systemic disease as evidenced by the clinical response to appropriate antibiotics.
Hyman
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D001431:Bacteriological Techniques; D001437:Bacteriuria / Q000382:microbiology*; D006095:Gram-Positive Cocci / Q000302:isolation & purification; D008853:Microscopy / Q000379:methods; D013194:Staining and Labeling