Cloning and characterization of an amplified DNA sequence in chromosomal DNA of Streptomyces aureofaciens 2201.
In strain 2201 of Streptomyces aureofaciens, a high copy number amplified DNA sequence (ADS-Sa2201) was found and characterized. The amplified sequence in the chromosomal DNA of this strain forms a stretch of about 10 kb tandemly repeated 100-500 times. In this strain also, extrachromosomal copies of the repeated unit of the ADS-Sa2201 were found. In cloning experiments any autonomous replicon was found on ADS-Sa2201 and it thus can be presumed that the presence of the extrachromosomal copies of the repeated unit of ADS-Sa2201 is only a result of its excision from the chromosome. A part of the repeated unit of ADS-Sa2201 was also found in chromosomal DNA of strain 13 of S. aureofaciens. In this strain no part of ADS-Sa2201 is present extrachromosomally.
Pristas
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Institute of Molecular Biology, Slovak Academy of Sciences, Bratislava, Czechoslovakia.
Godány
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D003001:Cloning, Molecular; D015342:DNA Probes; D004269:DNA, Bacterial / Q000235:genetics*; D005784:Gene Amplification; D005822:Genetic Vectors; D010957:Plasmids; D012091:Repetitive Sequences, Nucleic Acid; D012093:Replicon; D015183:Restriction Mapping; D013304:Streptomyces aureofaciens / Q000235:genetics*
D015342:DNA Probes; D004269:DNA, Bacterial