title: Myelination of regenerated axons in goldfish optic nerve by Schwann cells.
abstract: This study uses immunohistochemistry and EM to examine the site of injury in goldfish optic nerve during axonal regeneration. Within seven days of nerve crush axons begin to regrow and a network of GFAP+ reactive astrocytes appears in the nerve on either side of the injury. However, the damaged area remains GFAP-. By 42 days after nerve crush, the sheaths of new axons acquire myelin marker 6D2, and the crush area becomes populated by a mass of longitudinally-orientated S-100+ cells. Ultrastructurally, the predominant cells in the crush area bear a strong resemblance to peripheral nerve Schwann cells; they display a one-to-one association with myelinated axons, have a basal lamina and are surrounded by collagen fibres. It is proposed that these cells are Schwann cells which enter the optic nerve as a result of crush, where they become confined to the astrocyte-free crush area.
authors.affiliation: Department of Optometry and Vision Sciences, UMIST, Manchester, UK.
authors.forename: S N
authors.initials: SN
authors.lastname: Nona
authors.forename: A
authors.initials: A
authors.lastname: Duncan
authors.forename: C A
authors.initials: CA
authors.lastname: Stafford
authors.forename: A
authors.initials: A
authors.lastname: Maggs
authors.forename: G
authors.initials: G
authors.lastname: Jeserich
authors.forename: J R
authors.initials: JR
authors.lastname: Cronly-Dillon
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000502:physiology; D001369:Axons / Q000502:physiology / Q000648:ultrastructure; D015415:Biomarkers; D005455:Fluorescent Antibody Technique; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D006054:Goldfish; D008854:Microscopy, Electron; D009186:Myelin Sheath / Q000502:physiology* / Q000648:ultrastructure; D009409:Nerve Crush; D009416:Nerve Regeneration; D009900:Optic Nerve / Q000502:physiology* / Q000648:ultrastructure; D020221:Optic Nerve Injuries; D009418:S100 Proteins / Q000032:analysis; D012583:Schwann Cells / Q000502:physiology* / Q000648:ultrastructure
chemical_list: D015415:Biomarkers; D005904:Glial Fibrillary Acidic Protein; D009418:S100 Proteins