title: Platelets and vitronectin: immunocytochemical localization and platelet interaction with exogenously added vitronectin.
abstract: Vitronectin stores were localized in human platelets by immunofluorescence, confocal laser microscopy, and immunoelectron microscopy. In ultrathin cryosections, the immunogold labelling was mainly observed in the alpha-granules, although occasional immunolabelling of the platelet surface was present. The interaction between thrombin-stimulated platelets and exogenously added vitronectin was also explored. This vitronectin had been purified by heparin-affinity chromatography. Binding of vitronectin to thrombin-stimulated platelets was demonstrated by flow cytometry. The immunoelectron microscopical studies revealed that this binding was mainly restricted to clumps of released alpha-granular material on the platelet surface. The possible significance of the interaction between exogenous vitronectin and released platelet proteins is discussed.
authors.affiliation: Institute of Pathology, Rikshospitalet, Oslo, Norway.
authors.forename: M
authors.initials: M
authors.lastname: Røger
authors.forename: T S
authors.initials: TS
authors.lastname: Halstensen
authors.forename: K
authors.initials: K
authors.lastname: Høgåsen
authors.forename: T E
authors.initials: TE
authors.lastname: Mollnes
authors.forename: N O
authors.initials: NO
authors.lastname: Solum
authors.forename: T
authors.initials: T
authors.lastname: Hovig
mesh_terms: D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000276:immunology; D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D003594:Cytoplasmic Granules / Q000737:chemistry; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D006023:Glycoproteins / Q000097:blood*; D006801:Humans; D007150:Immunohistochemistry; D016253:Microscopy, Immunoelectron; D008969:Molecular Sequence Data; D010980:Platelet Membrane Glycoproteins / Q000276:immunology; D011485:Protein Binding; D013917:Thrombin / Q000494:pharmacology; D019096:Vitronectin
chemical_list: D000911:Antibodies, Monoclonal; D006023:Glycoproteins; D010980:Platelet Membrane Glycoproteins; D019096:Vitronectin; D013917:Thrombin