Precise excision of transposons and point mutations induced by chemicals.
The ability of 23 chemicals (carcinogens and non-carcinogens) to induce precise excision of Tn10 and point mutations was studied in experiments with a single strain. The mutation assay was shown to detect a wider spectrum of genotoxic agents than the assay of Tn10 precise excision. The latter was induced only by potent SOS mutagens, which is in accordance with data on the SOS dependence of the induction of precise excision of Tn10. The precise excision assay as an additional test contributing to the knowledge of particular features of the action of a tested mutagen is discussed. The induction of precise excision of Tn10 by pyrene (and its failure to induce point mutations in this strain) demonstrates the value of using the transposon excision assay in cases of 'problem' mutagens.
Rusina OYu
Gamaleya Institute for Epidemiology and Microbiology, Academy of Medical Sciences, Moscow, Russia.
Mirskaya
E E
EE
Andreeva
I V
IV
Skavronskaya
A G
AG
D015197:Carcinogenicity Tests; D004251:DNA Transposable Elements / Q000187:drug effects*; D009152:Mutagenicity Tests; D009153:Mutagens / Q000494:pharmacology*; D017354:Point Mutation; D013014:SOS Response, Genetics; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics*
D004251:DNA Transposable Elements; D009153:Mutagens