title: Inhibition or activation of human T cell receptor transfectants is controlled by defined, soluble antigen arrays.
abstract: We present evidence that direct T cell receptor (TCR) occupancy by antigen can either activate or inhibit T cells, depending upon whether or not a threshold number of local TCRs are crosslinked by multivalent arrays of the antigen. Variants of Jurkat cells were previously transfected with TCR alpha and beta chains that bind fluorescein, yielding FL-TCR+ human T cells. The transfectants are activated upon binding soluble multivalent antigen arrays at concentrations well below those required for monovalent interactions. This activation, measured by calcium fluxes and interleukin 2 (IL-2) production, indicates the superior binding avidity of multivalent ligands. Smaller, less multivalent arrays do not activate the cells, but antagonize larger arrays, demonstrating that antigen can bind TCR as either agonist or antagonist. The balance between activation and inhibition depends upon antigen array size, ligand valence, and concentration, indicating that a threshold extent of receptor crosslinking, and not individual perturbations of single TCR, is required for activation by antigen. Approximately 100 stimulatory arrays specifically bind per FL-TCR+ cell at concentrations where IL-2 production is half-maximal.
authors.affiliation: Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
authors.forename: D E
authors.initials: DE
authors.lastname: Symer
authors.forename: R Z
authors.initials: RZ
authors.lastname: Dintzis
authors.forename: D J
authors.initials: DJ
authors.lastname: Diamond
authors.forename: H M
authors.initials: HM
authors.lastname: Dintzis
mesh_terms: D000941:Antigens / Q000276:immunology*; D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D006801:Humans; D007376:Interleukin-2 / Q000096:biosynthesis; D008213:Lymphocyte Activation; D011948:Receptors, Antigen, T-Cell / Q000235:genetics / Q000502:physiology*; D013601:T-Lymphocytes / Q000276:immunology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014162:Transfection
chemical_list: D000941:Antigens; D007376:Interleukin-2; D011948:Receptors, Antigen, T-Cell; D013755:Tetradecanoylphorbol Acetate; D002118:Calcium