BALB/3T3 fibroblast-conditioned medium attracts cultured mast cells derived from W/W but not from mi/mi mutant mice, both of which are deficient in mast cells.
Proliferation of murine mast cells is induced by both T-cell-derived and fibroblast-derived growth factors. Because the most potent T-cell-derived mast cell growth factor, interleukin-3, promotes the migration of mast cells, we investigated whether fibroblast-derived growth factors had the chemoattractive activity as well. Conditioned medium (CM) of BALB/3T3 fibroblasts induced the migration of cultured mast cells (CMC) derived from normal (+/+) mice. BALB/3T3-CM contained the mast cell growth factor (MGF)/stem cell factor (SCF)/kit ligand (KL), which is the ligand for the receptor encoded by the W (c-kit) gene. CMC derived from the spleen of W/W mice lack the extracellular domain of the W (c-kit) receptor, and W/W CMC did not proliferate in response to BALB/3T3-CM. However, W/W CMC did migrate normally toward BALB/3T3-CM and, moreover, the antibody to the extracellular domain of the W (c-kit) receptor did not inhibit the chemoattractive activity of +/+ CMC toward BALB/3T3-CM. These results indicated that MGF/SCF/KL itself did not represent the major chemoattractive activity. On the other hand, BALB/3T3-CM induced neither proliferation nor migration of CMC derived from mi/mi mice. Both W/W and mi/mi mice are deficient in mast cells, but the present results suggest that the mechanism of the abnormality is different between W/W and mi/mi mice.
Jippo-Kanemoto
T
T
Department of Pathology, Osaka University Medical School, Japan.
Adachi
S
S
Ebi
Y
Y
Matsuda
H
H
Kasugai
T
T
Nishikawa
S
S
Kitamura
Y
Y
D000818:Animals; D002455:Cell Division; D002460:Cell Line; D002630:Chemotactic Factors / Q000502:physiology*; D002634:Chemotaxis, Leukocyte / Q000502:physiology; D003470:Culture Media / Q000494:pharmacology; D005346:Fibroblast Growth Factors / Q000502:physiology*; D005347:Fibroblasts / Q000378:metabolism*; D016298:Hematopoietic Cell Growth Factors / Q000235:genetics / Q000502:physiology; D008407:Mast Cells / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009154:Mutation; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000502:physiology; D019009:Proto-Oncogene Proteins c-kit; D013154:Spleen / Q000166:cytology; D019089:Stem Cell Factor
D002630:Chemotactic Factors; D003470:Culture Media; D016298:Hematopoietic Cell Growth Factors; D011518:Proto-Oncogene Proteins; D019089:Stem Cell Factor; D005346:Fibroblast Growth Factors; D019009:Proto-Oncogene Proteins c-kit