Appearance of interleukin-1 in macrophages and in ramified microglia in the brain of endotoxin-treated rats: a pathway for the induction of non-specific symptoms of sickness?
The presence and cellular localization of interleukin-1 beta immunoreactivity (irIL-1) in and around the brain was investigated using immunocytochemistry on Bouin's fixed vibratome brain sections of control and endotoxin-treated rats. Peripheral administration of endotoxin resulted in the appearance of irIL-1 in cells in the meninges, choroid plexus, brain blood vessels and in non-neuronal cells in the brain parenchyma. Using monoclonal and polyclonal antibodies to macrophage and astrocyte antigens, the endotoxin-induced irIL-1 positive cells could be identified as macrophages in the meninges and choroid plexus (ED2), perivascular cells (ED2) and ramified microglial cells (GSA-I-B4 isolectin). Our data demonstrate a pathway for the induction of non-specific sickness symptoms in response to endotoxin.
van Dam
A M
AM
Department of Pharmacology, Medical Faculty, Free University, Amsterdam, Netherlands.
Brouns
M
M
Louisse
S
S
Berkenbosch
F
F
D000818:Animals; D001921:Brain / Q000166:cytology / Q000378:metabolism*; D004731:Endotoxins / Q000494:pharmacology*; D007150:Immunohistochemistry; D007375:Interleukin-1 / Q000378:metabolism*; D008264:Macrophages / Q000378:metabolism*; D008297:Male; D009457:Neuroglia / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
D004731:Endotoxins; D007375:Interleukin-1