title: An endothelial ligand for L-selectin is a novel mucin-like molecule.
abstract: The adhesive interaction between circulating lymphocytes and the high endothelial venules (HEV) of lymph nodes (LN) is mediated by lymphocyte L-selectin, a member of the selectin family of cell adhesion proteins. Previous work has identified a sulfated 50 kd glycoprotein (Sgp50) as an HEV ligand for L-selectin. We now report the purification of this glycoprotein and the utilization of the derived N-terminal amino acid sequence to clone a cDNA. The predicted sequence reveals a novel, mucin-like molecule containing two serine/threonine-rich domains. The mRNA encoding this glycoprotein is preferentially expressed in LN. Antibodies against predicted peptides immunoprecipitate Sgp50 and stain the apical surface of LN HEV. These results thus define a tissue-specific mucin-like endothelial glycoprotein that appears to function as a scaffold that presents carbohydrates to the L-selectin lectin domain.
authors.affiliation: Department of Immunobiology, Genentech, Inc,, South San Francisco, California 94080.
authors.forename: L A
authors.initials: LA
authors.lastname: Lasky
authors.forename: M S
authors.initials: MS
authors.lastname: Singer
authors.forename: D
authors.initials: D
authors.lastname: Dowbenko
authors.forename: Y
authors.initials: Y
authors.lastname: Imai
authors.forename: W J
authors.initials: WJ
authors.lastname: Henzel
authors.forename: C
authors.initials: C
authors.lastname: Grimley
authors.forename: C
authors.initials: C
authors.lastname: Fennie
authors.forename: N
authors.initials: N
authors.lastname: Gillett
authors.forename: S R
authors.initials: SR
authors.lastname: Watson
authors.forename: S D
authors.initials: SD
authors.lastname: Rosen
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D005796:Genes; D019041:L-Selectin; D008024:Ligands; D051379:Mice; D008969:Molecular Sequence Data; D009077:Mucins / Q000235:genetics* / Q000378:metabolism*; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D011487:Protein Conformation
chemical_list: D015815:Cell Adhesion Molecules; D008024:Ligands; D009077:Mucins; D009838:Oligodeoxyribonucleotides; D019041:L-Selectin; C075185:sulfated glycoprotein p50; D004247:DNA