Optimization of cytotoxic assay by target cell retention of the fluorescent dye carboxyfluorescein diacetate (CFDA) and comparison with conventional 51CR release assay.
This paper describes the comparison between a fluorimetric NK assay based on the target cell retention of fluorescent dye carboxyfluorescein diacetate (cFDA) and standard 51Cr release assay. The results provide several suggestions to improve the cytotoxic assay based on the use of the fluorogenic substrate showing that the measurements of cFDA retained by target cells represent a method of evaluating cytotoxicity completely comparable to the 51Cr release assay.
Provinciali
M
M
Gerontology Research Department, Italian National Research Centres on Aging (I.N.R.C.A.), Ancona.
Di Stefano
G
G
Fabris
N
N
D002860:Chromium Radioisotopes; D003601:Cytotoxicity Tests, Immunologic / Q000379:methods*; D003602:Cytotoxicity, Immunologic / Q000276:immunology*; D005452:Fluoresceins; D005456:Fluorescent Dyes; D005470:Fluorometry; D006801:Humans; D007694:Killer Cells, Natural / Q000276:immunology; D014407:Tumor Cells, Cultured
D002860:Chromium Radioisotopes; D005452:Fluoresceins; D005456:Fluorescent Dyes; C027780:carboxyfluoresceindiacetate