Paraoxon block of chloride conductance in cell R2 of Aplysia californica.
In the presence of paraoxon, the amplitudes of chloride currents activated by acetylcholine (ACh) or gamma-aminobutyric acid (GABA) were reduced in cell R2 of Aplysia californica. IC50 values were 12 and 9.7 microM for ACh and GABA responses, respectively. Paraoxon did not affect resting membrane potential, input resistance, or chloride reversal potential. Both the slopes and maxima of ACh and GABA concentration-response curves were reduced by paraoxon, suggesting that paraoxon antagonism of these responses is not competitive. The antagonism of ACh and GABA responses by paraoxon was not related to inhibition of acetylcholinesterase.
Filbert
M G
MG
Neurotoxicology Branch, U.S. Army Medical Research Institute of Chemical Defense, Aberdeen Proving Ground, MD 21010-5425.
Apland
J P
JP
Petrali
J P
JP
Adler
M
M
D000109:Acetylcholine / Q000494:pharmacology; D000110:Acetylcholinesterase / Q000378:metabolism; D000818:Animals; D001048:Aplysia / Q000378:metabolism*; D002478:Cells, Cultured; D002712:Chlorides / Q000378:metabolism*; D004594:Electrophysiology; D005724:Ganglia / Q000187:drug effects / Q000378:metabolism; D006651:Histocytochemistry; D007473:Ion Channels / Q000187:drug effects / Q000378:metabolism*; D008564:Membrane Potentials / Q000187:drug effects; D010261:Paraoxon / Q000494:pharmacology*; D005680:gamma-Aminobutyric Acid / Q000494:pharmacology
D002712:Chlorides; D007473:Ion Channels; D005680:gamma-Aminobutyric Acid; D000110:Acetylcholinesterase; D000109:Acetylcholine; D010261:Paraoxon