Immunoelectron microscopic detection of the hepatitis B virus major surface protein in dilated perinuclear membranes of yeast cells.
The major surface protein of hepatitis B virus produced in Saccharomyces cerevisiae can be recovered from cell lysates in the form of 22-mm lipoprotein particles. Immunoelectron microscopy was applied to investigate site and time of particle assembly. Thin sections of yeast cells revealed that production of the S protein provoked a dilation of the endoplasmic reticulum. Dilated areas were specifically labeled with a polyclonal antibody raised against glutaraldehyde-treated yeast-derived HBsAg particles. In contrast to previous postulates of particle formation during cell lysis and extract preparation, these results suggest that particle formation in yeast occurs in the endoplasmic reticulum and that transport of particles along the secretion pathway is blocked.
Biemans
R
R
Research Department, SmithKline Beecham Biologicals, Rixensart, Belgium.
Thines
D
D
Petre-Parent
B
B
De Wilde
M
M
Rutgers
T
T
Cabezon
T
T
D002472:Cell Transformation, Viral; D004721:Endoplasmic Reticulum / Q000648:ultrastructure*; D006514:Hepatitis B Surface Antigens / Q000648:ultrastructure*; D007181:Inclusion Bodies, Viral / Q000648:ultrastructure*; D016253:Microscopy, Immunoelectron; D012441:Saccharomyces cerevisiae; D014771:Virion / Q000648:ultrastructure; D014779:Virus Replication
D006514:Hepatitis B Surface Antigens