Detection of endothelin-1 mRNA by RT-PCR in isolated rat renal tubules.
A combination of reverse transcription and polymerase chain reaction was utilized to detect baseline endothelin-1 (ET-1) mRNA expressed in renal tubules dissected from rats. 5' and 3' primers were constructed according to human ET-1 genomic DNA. Rat ET-1 mRNA was found to be expressed only in cortical through medullary collecting ducts in addition to glomeruli. Sites for tubular synthesis of ET-1 corroborate well with major ET-1 binding sites along the nephron, indicating autocrine/paracrine role of ET-1 in the renal tubules and supporting a prevailing concept on such function of ET-1 in many differing tissues.
Uchida
S
S
Fourth Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Japan.
Takemoto
F
F
Ogata
E
E
Kurokawa
K
K
D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D016232:Endothelins / Q000235:genetics*; D007672:Kidney Cortex / Q000502:physiology; D007678:Kidney Glomerulus / Q000502:physiology; D007679:Kidney Medulla / Q000502:physiology; D007684:Kidney Tubules / Q000502:physiology*; D007685:Kidney Tubules, Collecting / Q000502:physiology; D008297:Male; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000032:analysis* / Q000235:genetics; D012194:RNA-Directed DNA Polymerase; D051381:Rats; D017208:Rats, Wistar
D016232:Endothelins; D009838:Oligodeoxyribonucleotides; D012333:RNA, Messenger; D004247:DNA; D012194:RNA-Directed DNA Polymerase