title: Vicilin-like seed storage proteins in the gymnosperm interior spruce (Picea glauca/engelmanii).
abstract: A seed storage protein cDNA was characterized from a library of interior spruce (Picea glauca/engelmanii complex) cotyledonary stage somatic embryos. The deduced amino acid sequence predicts a 448 amino acid (50 kDa) polypeptide with 28-38% identity with angiosperm vicilin-like 7S globulins. XXC/G codon usage is low (47%) relative to monocot angiosperms while pairwise comparisons show that spruce, monocot, and dicot vicilins are approximately equal in amino acid divergence. Although small by comparison, the spruce vicilin contains an N terminal hydrophilic region characteristic of angiosperm 'large' vicilins. Genomic Southern blotting predicts that the cDNA is encoded by a gene family.
authors.affiliation: Forest Biotechnology Centre, B.C. Research Corporation, Vancouver, Canada.
authors.forename: C H
authors.initials: CH
authors.lastname: Newton
authors.forename: B S
authors.initials: BS
authors.lastname: Flinn
authors.forename: B C
authors.initials: BC
authors.lastname: Sutton
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D004247:DNA; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics*; D014674:Plant Proteins, Dietary / Q000235:genetics; D055314:Seed Storage Proteins; D012639:Seeds / Q000235:genetics; D017386:Sequence Homology, Amino Acid
chemical_list: D010940:Plant Proteins; D014674:Plant Proteins, Dietary; D055314:Seed Storage Proteins; D004247:DNA; C015575:vicilin protein, plant