Localization and retention in vitro of fluorescently labeled aortic baroreceptor terminals on neurons from the nucleus tractus solitarius.
The anterograde fluorescent tracer DiA was used to visualize baroreceptor fibers and synaptic terminals both in living and fixed tissue. Baroreceptor fibers labeled with DiA terminated as a dense synaptic field in the medial nucleus tractus solitarius (NTS), making synaptic contact on the soma, as well as processes of neurons that they innervated. A similar distribution and morphology was observed in baroreceptor fibers and terminals labeled with horseradish peroxidase. DiA also identified baroreceptor terminals and the neurons receiving these synaptic contacts in vitro. NTS neurons were dissociated from their surrounding tissue and identified by attached baroreceptor terminals that retained the fluorescent dye. These results will enable us to study the electrophysiological properties of dispersed neurons that receive identified baroreceptor synaptic terminals.
Mendelowitz
D
D
Department of Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, TX 77030.
Yang
M
M
Andresen
M C
MC
Kunze
D L
DL
D000818:Animals; D001011:Aorta / Q000294:innervation*; D001370:Axonal Transport; D005456:Fluorescent Dyes; D006735:Horseradish Peroxidase; D008526:Medulla Oblongata / Q000033:anatomy & histology* / Q000166:cytology; D009131:Muscle, Smooth, Vascular / Q000294:innervation; D009411:Nerve Endings / Q000648:ultrastructure*; D009412:Nerve Fibers / Q000648:ultrastructure; D009474:Neurons / Q000166:cytology*; D011311:Pressoreceptors / Q000033:anatomy & histology* / Q000166:cytology; D011726:Pyridinium Compounds; D051381:Rats; D013569:Synapses / Q000648:ultrastructure
D005456:Fluorescent Dyes; D011726:Pyridinium Compounds; C076868:4-(4-dihexadecylaminostyryl)-N-methylpyridium; D006735:Horseradish Peroxidase