Immunoelectron microscopic studies reveal differences in distribution of sialo-oligosaccharide receptors for Mycoplasma pneumoniae on the epithelium of human and hamster bronchi.
Long-chain sialo-oligosaccharides with poly-N-acetyllactosamine backbones (Ii antigen type) are major host cell receptors for the human pathogen Mycoplasma pneumoniae. Previous immunofluorescence studies of the human bronchial epithelium, using sequence-specific monoclonal antibodies to the branched I-type and linear i-type backbones, have indicated that sialylated and nonsialylated long-chain sequences of both types are richly expressed on the ciliated cells, where they are polarized at the apical aspects. These sequences are lacking in the goblet cells. In the present study, the display of these oligosaccharides has been investigated by electron microscopy (immunogold labelling) in the human bronchial epithelium and in that of the hamster, an animal model commonly used for M. pneumoniae infection. In the human bronchial epithelium, the long-chain branched sequences have been detected along the entire length of the cilia and on microvilli, whereas the linear sequences are confined to the microvilli and the basal aspects of the cilia. On the ciliated epithelial cells of the hamster, by contrast, the branched and linear sequences (sialo- and asialo-) have been detected exclusively on microvilli. A further striking difference is that in the hamster these structures are expressed in abundance on the goblet cells and in the intracellular globules. We suggest that the latter finding may partly explain the relatively large doses of M. pneumoniae required to establish experimental infection in the hamster, as the receptor-bearing secreted mucus may have a protective role in binding to the microorganisms, leading to their clearance by bronchociliary action.
Loveless
R W
RW
Glycoconjugates Section, MRC Clinical Research Centre, Harrow, Middlesex, United Kingdom.
Griffiths
S
S
Fryer
P R
PR
Blauth
C
C
Feizi
T
T
D000818:Animals; D001980:Bronchi / Q000737:chemistry* / Q000648:ultrastructure; D002240:Carbohydrate Sequence; D006224:Cricetinae; D004848:Epithelium / Q000737:chemistry / Q000648:ultrastructure; D006801:Humans; D007048:I Blood-Group System; D008647:Mesocricetus; D016253:Microscopy, Immunoelectron; D008969:Molecular Sequence Data; D009177:Mycoplasma pneumoniae / Q000502:physiology*; D009844:Oligosaccharides / Q000032:analysis*
D007048:I Blood-Group System; D009844:Oligosaccharides; C016735:sialooligosaccharides