title: Characterization of the colorectal antigen IOR-C2.
abstract: A colorectal antigen (IOR-C2) was characterized by a monoclonal antibody produced against the colon cancer cell line SW1116. By immunohistochemical staining the antigen was abundant and strongly expressed in epithelium of normal colon whereas colorectal carcinomas showed a more variable and heterogenous reactivity to the antibody (IOR-C2). Radioimmunoprecipitates of SW1116 cell homogenates showed a 160-200 kD band in SDS gels. Physicochemical characterization indicate that at least two IOR-C2 reactive sites are present on the antigen tested and that it is mainly an 0-linked glycoprotein carbohydrate chain which can also be N-linked to the protein. The expression of IOR-C2 mimics that of the colon associated antigen (CAA) and NCC-CO-450 antigen but is distinct from these with regard to its expression in carcinomas as well as its physicochemical characteristics.
authors.affiliation: National Institute of Oncology, C. Habana, Cuba.
authors.forename: A M
authors.initials: AM
authors.lastname: Vazquez
authors.forename: B
authors.initials: B
authors.lastname: Tormo
authors.forename: A
authors.initials: A
authors.lastname: Velandia
authors.forename: R
authors.initials: R
authors.lastname: Giscombe
authors.forename: I
authors.initials: I
authors.lastname: Ansotegui
authors.forename: M
authors.initials: M
authors.lastname: Jeddi-Tehrani
authors.forename: R
authors.initials: R
authors.lastname: Perez
authors.forename: H
authors.initials: H
authors.lastname: Mellstedt
authors.forename: P
authors.initials: P
authors.lastname: Biberfeld
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor / Q000276:immunology; D015179:Colorectal Neoplasms / Q000276:immunology*; D000939:Epitopes; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000276:immunology; D051379:Mice; D014407:Tumor Cells, Cultured / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D000939:Epitopes