Modifications of the guanidine hydrochloride procedure for the extraction of RNA: isolation from a variety of tissues and adherent/nonadherent cell types.
In a previous report dealing with the guanidine hydrochloride protocol for the extraction of RNA from mouse peritoneal macrophages, we identified a major source of RNA-degrading activity and showed that its removal early in the extraction procedure resulted in a more dependable method for the recovery of high-quality RNA. This report extends these findings and demonstrates the general applicability of the technique to a variety of fresh or frozen adherent cell types, cell suspensions and tissues, further highlighting stages at which degradation is most likely to occur and how to avoid a variety of pitfalls associated with the extraction procedure.
Kamdar
S J
SJ
Jackson Laboratory, Bar Harbor, ME 04609.
Evans
R
R
D000818:Animals; D002448:Cell Adhesion; D002478:Cells, Cultured; D015925:Cryopreservation; D005821:Genetic Techniques; D019791:Guanidine; D006146:Guanidines; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D012313:RNA / Q000302:isolation & purification*
D006146:Guanidines; D012313:RNA; D019791:Guanidine