title: MHC and malaria: the relationship between HLA class II alleles and immune responses to Plasmodium falciparum.
abstract: In mice, immune responses to subunits of defined malaria antigens are regulated by genes mapping within the MHC and it has been suggested that such genetic restriction will be a major obstacle in the development of a human malaria vaccine. The relationship between class II human leukocyte antigen (HLA) genes and immune recognition of three candidate antigens for a vaccine against Plasmodium falciparum malaria has been investigated in a human population living in a malaria endemic area of West Africa. The study population was shown to be extremely heterogeneous for HLA class II alleles and marked differences in allelic frequency were detected between members of different ethnic groups. One class II DQA-DQB combination (serological specificity DQw2) was particularly common among members of the Fula ethnic group. This haplotype was significantly associated with higher than average levels of antibody to a peptide epitope, (EENV)6, of the malaria antigen Pf155/RESA. There was little evidence of association between HLA class II genotype and cellular proliferative or interferon gamma responses to the antigens tested. Overall, the number of significant associations between immune responses and specific HLA class II haplotypes was greater than would be expected by chance but less than would be expected if class II-dependent genetic restriction were a major factor governing human immune responses to malaria antigens. Thus, although some qualitative variation in the immune response to vaccine antigens may occur in ethnically different target populations, widespread HLA-associated nonresponsiveness to a multivalent subunit malaria vaccine is unlikely.
authors.affiliation: Medical Research Council Laboratories, Fajara, The Gambia.
authors.forename: E M
authors.initials: EM
authors.lastname: Riley
authors.forename: O
authors.initials: O
authors.lastname: Olerup
authors.forename: S
authors.initials: S
authors.lastname: Bennett
authors.forename: P
authors.initials: P
authors.lastname: Rowe
authors.forename: S J
authors.initials: SJ
authors.lastname: Allen
authors.forename: M J
authors.initials: MJ
authors.lastname: Blackman
authors.forename: M
authors.initials: M
authors.lastname: Troye-Blomberg
authors.forename: A A
authors.initials: AA
authors.lastname: Holder
authors.forename: B M
authors.initials: BM
authors.lastname: Greenwood
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000483:Alleles; D000818:Animals; D000913:Antibodies, Protozoan / Q000032:analysis*; D002648:Child; D002675:Child, Preschool; D005802:Genes, MHC Class II; D006683:HLA-DQ Antigens / Q000235:genetics; D006684:HLA-DR Antigens / Q000235:genetics; D006239:Haplotypes; D006801:Humans; D008875:Middle Aged; D010963:Plasmodium falciparum / Q000276:immunology*
chemical_list: D000913:Antibodies, Protozoan; D006683:HLA-DQ Antigens; C053186:HLA-DQ2 antigen; D006684:HLA-DR Antigens