Mechanism and regulation of synthesis of aerobactin in Escherichia coli K12 (pColV-K30).
The aerobactin operon of the virulence plasmid pColV-K30 of Escherichia coli K12 consists of four genes for biosynthesis and one for transport of the siderophore. Regulation by iron occurs at the transcriptional level and is mediated by a ferrous iron binding protein designated Fur (ferric uptake regulation). The metallated Fur repressor binds at a palindromic dyad, the "iron box" operator, situated in the vicinity of the RNA polymerase attachment site of the promoter. Evidence suggests that the ferrous iron enters the C-terminal domain of Fur to cause a conformational change in the N-terminal part of the protein. This results in greatly enhanced affinity of the repressor for the operator.
Neilands
J B
JB
Department of Molecular and Cellular Biology, University of California, Berkeley 94720.
D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D004758:Enterobactin / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics* / Q000472:pathogenicity; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics*; D006877:Hydroxamic Acids / Q000378:metabolism*; D007501:Iron / Q000378:metabolism; D008969:Molecular Sequence Data; D009876:Operon; D010957:Plasmids; D012097:Repressor Proteins / Q000235:genetics; D017262:Siderophores / Q000096:biosynthesis / Q000235:genetics*; D014774:Virulence
D001426:Bacterial Proteins; D006877:Hydroxamic Acids; D012097:Repressor Proteins; D017262:Siderophores; C068477:ferric uptake regulating proteins, bacterial; C031819:aerobactin; D004758:Enterobactin; D007501:Iron