Hemoglobin A1c by HPLC with the Pharmacia Mono S HR 5/N cation-exchange column: influence of sample protein load on optimal chromatographic conditions.
The Pharmacia Mono S HR 5/5 column has been optimized for hemoglobin A1c analysis by HPLC by using a much smaller column load, decreased buffer flow rate, and a steeper gradient than was used in previously described methods. Superior chromatographic separation, shorter analysis time, and a greatly extended column life have resulted.
Philcox
J C
JC
Division of Clinical Chemistry, Institute of Medical and Veterinary Science, Adelaide, South Australia.
Haywood
M R
MR
Rofe
A M
AM
D002412:Cations; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002852:Chromatography, Ion Exchange; D005319:Fetal Hemoglobin / Q000302:isolation & purification; D006442:Glycated Hemoglobin / Q000302:isolation & purification*; D006441:Hemoglobin A / Q000302:isolation & purification; D006801:Humans
D002412:Cations; D005319:Fetal Hemoglobin; D006442:Glycated Hemoglobin; D006441:Hemoglobin A