Histochemical discrimination of fibers in regenerating rat infraorbital nerve.
In rat dorsal root ganglia, histochemical staining of carbonic anhydrase (CA) and cholinesterase (CE) yields a reciprocal pattern of activity: Sensory processes are CA positive and CE negative, whereas motor processes are CA negative and CE positive. In rat infraorbital nerve (a sensory peripheral nerve), we saw extensive CA staining of nearly 100% of the myelinated axons. Although CE reactivity in myelinated axons was extremely rare, we did observe CE staining of unmyelinated autonomic fibers. Four weeks after transection of infraorbital nerves, CA-stained longitudinal sections of the proximal stump demonstrated 3 distinct morphological zones. A fraction of the viable axons retained CA activity to within 2 mm of the distal extent of the stump, and the stain is capable of resolving growth sprouts being regenerated from these fibers. Staining of unmyelinated autonomic fibers in serial sections shows that CE activity was not retained as far distally as is the CA sensory staining.
Wilke
R A
RA
Department of Cellular Biology and Anatomy, Medical College of Wisconsin, Milwaukee.
Riley
D A
DA
Sanger
J R
JR
D000818:Animals; D001369:Axons / Q000648:ultrastructure; D002256:Carbonic Anhydrases; D002802:Cholinesterases; D005727:Ganglia, Spinal / Q000648:ultrastructure; D006651:Histocytochemistry; D008297:Male; D008442:Maxillary Nerve / Q000648:ultrastructure; D009046:Motor Neurons / Q000648:ultrastructure*; D009410:Nerve Degeneration; D009412:Nerve Fibers / Q000648:ultrastructure*; D009413:Nerve Fibers, Myelinated / Q000648:ultrastructure; D009416:Nerve Regeneration / Q000502:physiology; D009475:Neurons, Afferent / Q000648:ultrastructure*; D009915:Orbit / Q000294:innervation*; D051381:Rats; D011919:Rats, Inbred Strains; D013126:Spinal Nerve Roots / Q000648:ultrastructure; D013194:Staining and Labeling
D002802:Cholinesterases; D002256:Carbonic Anhydrases