title: Comparison of cell proliferation in breast carcinoma using image analysis (Ki-67) and flow cytometric systems.
abstract: Cellular proliferation has been implicated as an important predictor of biologic behavior in breast cancer. Cellular proliferation of 95 breast carcinomas was evaluated by comparing Ki-67 immunoreactivity in frozen sections quantitated by image analysis with S-phase and S + G2/M phase fraction determined by flow cytometry on nuclei extracted from fixed, paraffin-embedded sections (modified Hedley's technique). These parameters were correlated with traditional morphologic features of histologic grade, including mitotic count. Ki-67 immunoreactivity correlated with S-phase fraction determined by flow cytometry (r = .41, P = .001) and with S + G2/M phase fraction determined by flow cytometry (r = .29, P = .008). There was also a correlation between histologic grade and Ki-67 immunoreactivity (r = .30, P = .004) and between histologic grade and S-phase fraction (r = .42, P = .0001). Mitotic count correlated with Ki-67 immunoreactivity (r = .25, P = .015) and with S-phase fraction (r = .35, P = .001). Image and flow cytometric analysis systems provide comparable measurements of cellular proliferation; their measurements correlate with histologic grade and mitotic count in breast cancer.
authors.affiliation: Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, Georgia.
authors.forename: J C
authors.initials: JC
authors.lastname: Kennedy
authors.forename: N
authors.initials: N
authors.lastname: el-Badawy
authors.forename: P B
authors.initials: PB
authors.lastname: DeRose
authors.forename: C
authors.initials: C
authors.lastname: Cohen
mesh_terms: D001943:Breast Neoplasms / Q000473:pathology*; D002277:Carcinoma / Q000473:pathology*; D002455:Cell Division; D005260:Female; D005434:Flow Cytometry; D016195:G2 Phase; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007150:Immunohistochemistry; D019394:Ki-67 Antigen; D008938:Mitosis; D009687:Nuclear Proteins / Q000276:immunology / Q000302:isolation & purification*; D012189:Retrospective Studies; D016196:S Phase
chemical_list: D019394:Ki-67 Antigen; D009687:Nuclear Proteins