Interleukin-8 inhibits the induction of nitric oxide synthase in rat peritoneal neutrophils.
The effect of interleukin(IL)-8 and leukotriene B4 (LTB4) on the induction of nitric oxide (NO) synthase in rat peritoneal neutrophils (PMN) ex vivo was studied. IL-8, but not LTB4, caused concentration-dependent inhibition of the induction of a Ca(2+)-independent NO synthase ex vivo. The effect of IL-8 was not attributable to the synthesis of an inhibitor of this enzyme. These findings suggest complex regulatory control of the induction of NO synthase by cytokines.
McCall
T B
TB
Wellcome Research Laboratories, Beckenham, Kent, U.K.
Palmer
R M
RM
Moncada
S
S
D000594:Amino Acid Oxidoreductases / Q000096:biosynthesis*; D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002478:Cells, Cultured; D003600:Cytosol / Q000378:metabolism; D004790:Enzyme Induction / Q000187:drug effects; D016209:Interleukin-8 / Q000494:pharmacology*; D007700:Kinetics; D007975:Leukotriene B4 / Q000494:pharmacology; D008297:Male; D009504:Neutrophils / Q000187:drug effects / Q000201:enzymology*; D019001:Nitric Oxide Synthase; D051381:Rats; D011919:Rats, Inbred Strains
D016209:Interleukin-8; D007975:Leukotriene B4; D019001:Nitric Oxide Synthase; D000594:Amino Acid Oxidoreductases; D002118:Calcium