Persistently elevated frequency of spontaneous mutations in progeny of CHO clones surviving X-irradiation: association with delayed reproductive death phenotype.
Certain clonal progeny of Chinese hamster ovary (CHO) cells surviving X-irradiation demonstrate pleomorphic changes including a persistently decreased cloning efficiency, a dominant phenotype we have termed delayed reproductive death (Chang and Little, 1991, 1992b). We now report that cells from these progeny clones show a persistently elevated frequency of spontaneous mutations at the hprt locus for up to 95-100 population doublings post-irradiation. Mutant fractions as high as 10(-3) were scored, more than two orders of magnitude higher than those observed in clonal progeny of non-irradiated cells studied in parallel. These results are discussed in terms of the hypothesis that radiation induces a type of genetic instability among some surviving cells that results in a heritable mutator phenotype, and that this instability may also be involved in the phenomenon of delayed reproductive death.
Chang
W P
WP
Laboratory of Radiobiology, Harvard University School of Public Health, Boston, MA 02115.
Little
J B
JB
D000818:Animals; D016466:CHO Cells / Q000201:enzymology / Q000528:radiation effects; D002453:Cell Cycle / Q000528:radiation effects; D016923:Cell Death; D002470:Cell Survival / Q000235:genetics / Q000528:radiation effects*; D002999:Clone Cells / Q000201:enzymology / Q000528:radiation effects; D006224:Cricetinae; D003412:Cricetulus; D005804:Genes, Lethal; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics*; D009154:Mutation; D010641:Phenotype
D007041:Hypoxanthine Phosphoribosyltransferase