Alterations of the levels of glycoproteins Ib-IX and IIb-IIIa in platelets stored at 22 degrees C.
Platelets stored in CLX blood bags, under normal blood banking conditions, were studied for up to 7 days to determine if changes occurred in the levels of membrane glycoproteins (GP) Ib-IX and IIb-IIIa. Radiolabeled monoclonal antibodies (MAB) were used to estimate the number of glycoprotein molecules on the surface membrane of intact platelets. GP IX and GP IIb-IIIa levels remained essentially unaltered during storage. In contrast, the content of GP Ib at day 7 decreased by 45% of the total when fresh. The aggregation response to ristocetin, which requires GP Ib, was also diminished after 7 days. Addition of protease inhibitors, leupeptin and/or aprotinin did not appear to influence the degradation of this glycoprotein. We conclude that storage at 22 degrees C had deleterious effects on the GP Ib content of platelets.
Ribeiro
A
A
NSW Red Cross Blood Transfusion Service, Sydney, Australia.
Swann
J C
JC
Berndt
M C
MC
D000911:Antibodies, Monoclonal; D007611:Aprotinin; D001792:Blood Platelets / Q000737:chemistry*; D001793:Blood Preservation; D003080:Cold Temperature; D006801:Humans; D007976:Leupeptins; D010974:Platelet Aggregation / Q000187:drug effects; D019038:Platelet Glycoprotein GPIb-IX Complex; D010980:Platelet Membrane Glycoproteins / Q000032:analysis* / Q000378:metabolism; D012310:Ristocetin
D000911:Antibodies, Monoclonal; D007976:Leupeptins; D019038:Platelet Glycoprotein GPIb-IX Complex; D010980:Platelet Membrane Glycoproteins; C023592:glycocalicin; D012310:Ristocetin; D007611:Aprotinin; C032854:leupeptin