Murine Pneumocystis carinii adherence to vertical monolayers of cultured mink lung cells (MiCl1).
We describe a method for adherence and culture of murine Pneumocystis carinii in mink lung cells (MiCl1) grown on vertical supports. The vertical cultures were infected with P. carinii; the surrounding medium and inoculum were stirred to ensure circulation and contact with MiCl1 cells. When compared with conventional horizontal culture, the vertical method offers a more suitable system for assessing P. carinii adherence. This approach has proved suitable for quantitative evaluation of P. carinii adherence to MiCl1 cells in the presence of inhibitors.
Garner
R E
RE
Department of Basic Medical Sciences, Mercer University School of Medicine, Macon, Georgia 31207.
Walker
A N
AN
Horst
M N
MN
D000818:Animals; D002448:Cell Adhesion / Q000502:physiology*; D002478:Cells, Cultured; D046508:Culture Techniques / Q000379:methods; D008168:Lung / Q000382:microbiology*; D008907:Mink / Q000382:microbiology*; D011010:Pneumocystis / Q000254:growth & development*