title: Pharmacokinetics and excretion of phenol red in the channel catfish.
abstract: 1. Disposition of phenol red was examined in channel catfish (Ictalurus punctatus) after oral or intravascular (i.v.) dosing at 10 mg/kg body weight. 2. Phenol red was not detectable in plasma, urine, or bile after oral administration. 3. After i.v. dosing, plasma concentrations of phenol red were best described by a two-compartment pharmacokinetic model with distribution and elimination half-lives of 2.3 and 21 min, respectively. The apparent volume of distribution at steady state (Vss) was 225 ml/kg and total body clearance (Clb) was 658 ml/h per kg. Plasma protein binding was 19%. 4. Biliary excretion was the primary route of elimination of phenol red; in 24 h, 55% of the i.v. dose was excreted in bile compared with 24% in urine. No metabolites were detected in these fluids. 5. The use of anaesthesia during dosing had no effect on the quantitative excretion of phenol red by renal or biliary routes.
authors.affiliation: Division of Seafood Research, US Food and Drug Administration, Dauphin Island, AL 36528.
authors.forename: S M
authors.initials: SM
authors.lastname: Plakas
authors.forename: G R
authors.initials: GR
authors.lastname: Stehly
authors.forename: L
authors.initials: L
authors.lastname: Khoo
mesh_terms: D000284:Administration, Oral; D062365:Aminobenzoates / Q000494:pharmacology; D000777:Anesthetics / Q000494:pharmacology; D000818:Animals; D001646:Bile / Q000378:metabolism; D001659:Biliary Tract / Q000378:metabolism; D003080:Cold Temperature; D007059:Ictaluridae / Q000378:metabolism*; D010637:Phenolsulfonphthalein / Q000493:pharmacokinetics*
chemical_list: D062365:Aminobenzoates; D000777:Anesthetics; C003636:tricaine; D010637:Phenolsulfonphthalein