Detection and measurement of cytokines.
Accurate and sensitive methods for the measurement and detection of cytokines are an obvious pre-requisite for the study of cytokine biology, biochemistry and the possible involvement of these molecules in pathology. In this review, the various methods available for cytokine measurement and detection (bioassays, immunoassays and other procedures) are described and compared. A critical appraisal of the potential advantages and limitations of the techniques is included.
Thorpe
R
R
Division of Immunobiology, National Institute for Biological Standards and Control, Potters Bar, Hertfordshire, UK.
Wadhwa
M
M
Bird
C R
CR
Mire-Sluis
A R
AR
D000818:Animals; D001681:Biological Assay; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D016207:Cytokines / Q000032:analysis* / Q000235:genetics / Q000502:physiology; D006801:Humans; D007118:Immunoassay; D051379:Mice; D011237:Predictive Value of Tests; D011817:Rabbits; D012015:Reference Standards; D012680:Sensitivity and Specificity
D016207:Cytokines