Partial characterization, progressive development and correlations of some neoplastic characters in 20-methylcholanthrene-induced transformed murine embryonal fibroblasts.
Non-trypsinized primary fibroblast cells from 20-day-old Swiss mouse embryo was transformed by 20-methylcholanthrene (MCA) and was designated as CNCI-PM-20. The progressive development of some transformation related characters such as morphological alterations, reduced population doubling time, increased saturation density, reduced serum requirement, increased plating efficiency, loss of density dependent growth inhibition, anchorage-independent growth and tumorigenicity in mice clearly demonstrated the multistep process of carcinogenesis as well as the neoplastic nature of the cell line. Furthermore, the association of reduced requirement of serum and loss of density-dependent growth inhibition with tumorigenicity were also observed. Finally, anchorage-independent growth, greater malignant nature of transformed foci and increased number of giant cells may be required for tumorigenicity of this cell line.
Mukherjee
P
P
Department of Tissue Culture, Chittaranjan National Cancer Institute (Research Unit), Calcutta, India.
Das
S K
SK
D000818:Animals; D001790:Blood Physiological Phenomena; D002452:Cell Count; D002455:Cell Division; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D004622:Embryo, Mammalian; D005260:Female; D005347:Fibroblasts / Q000473:pathology; D008297:Male; D008748:Methylcholanthrene; D051379:Mice; D009368:Neoplasm Transplantation; D011247:Pregnancy
D008748:Methylcholanthrene