title: Sequence-tagged sites (STSs) for a set of mapped markers on chromosome 21.
abstract: Sequence tagged sites (STSs) have been proposed as a "common language" for comparing physical and genetic maps of the human genome produced by a variety of techniques. We have produced 44 STSs from 38 mapped loci on human chromosome 21. The STSs represent most of the loci designated as genetic reference or ordered physical framework markers, along with a number of others chosen to span all regions of 21q. Of the STSs, 12 are from gene segments, including 4 from exons of the APP gene encoding the amyloid beta protein precursor, and 32 mark anonymous DNA loci. These STSs make each of the corresponding loci readily accessible to the research community without the need for exchange of clones. These sites also represent multiple start points for the isolation of YAC clones that should permit overlapping the entire chromosome 21 long arm as cloned DNA.
authors.affiliation: Department of Neurology, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts 02114.
authors.forename: R E
authors.initials: RE
authors.lastname: Tanzi
authors.forename: D M
authors.initials: DM
authors.lastname: Romano
authors.forename: R
authors.initials: R
authors.lastname: Berger
authors.forename: M J
authors.initials: MJ
authors.lastname: Buraczynska
authors.forename: S M
authors.initials: SM
authors.lastname: Gaston
authors.forename: D M
authors.initials: DM
authors.lastname: Kurnit
authors.forename: D
authors.initials: D
authors.lastname: Patterson
authors.forename: J F
authors.initials: JF
authors.lastname: Gusella
authors.forename: G D
authors.initials: GD
authors.lastname: Stewart
mesh_terms: D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D002891:Chromosomes, Human, Pair 21; D004277:DNA, Single-Stranded; D005819:Genetic Markers; D006801:Humans; D006822:Hybrid Cells; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D016324:Sequence Tagged Sites
chemical_list: D004277:DNA, Single-Stranded; D005819:Genetic Markers