Intracellular distribution of the 126K/183K and capsid proteins in cells infected by some tobamoviruses.
Leaves of plants infected by tobacco mosaic virus (TMV) strain U1, TMV strain M, tomato mosaic virus strain Dahlemense and tobacco mild green mosaic virus strain U2 were examined for the presence and intracellular distribution of their capsid and 126K/183K proteins by immunoblotting and immunogold electron microscopy. The bulk of the capsid protein was found in the virus bundles (crystals), although small amounts were found in the chloroplasts and nuclei of cells infected by some of these tobamoviruses. The 126K protein of TMV-U1 and -M was localized in the X-bodies, whereas in cells infected by the other two viruses which induce no X-bodies, the 126K protein was found to be associated with virus bundles.
Das
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Department of Biological Sciences, Wayne State University, Detroit, Michigan 48202.
Hari
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D000595:Amino Acid Sequence; D002213:Capsid / Q000276:immunology / Q000302:isolation & purification; D014644:Genetic Variation; D007150:Immunohistochemistry; D016253:Microscopy, Immunoelectron; D008969:Molecular Sequence Data; D009029:Mosaic Viruses / Q000276:immunology*; D015388:Organelles; D010935:Plant Diseases / Q000382:microbiology*; D014027:Tobacco Mosaic Virus / Q000276:immunology; D014764:Viral Proteins / Q000276:immunology / Q000302:isolation & purification*
D014764:Viral Proteins