title: Relation between the number of sperms to be deposited and pregnancy rate in tubal insemination in rabbits.
abstract: Pregnancy rate of tubal insemination with semen diluted to concentrations of x10(0) to x10(7) was studied in rabbits. The animals were laparotomized under general anesthesia, and 0.05 ml of diluted semen was injected with a glass capillary from the tubal fimbria into the oviduct. The semen was obtained from mature male rabbits by means of an artificial vagina and diluted to concentrations between x10(0) and x10(7). One hour after the insemination, ovulation was induced by administration of hCG. Six experimental groups were set up according to the semen concentrations. In the x10(0), x10(2), x10(4), and x10(7) groups, newborn rabbits were obtained. The best pregnancy rate was obtained with the x10(2) groups, in which 10(5) level of sperms were deposited. The newborn rabbits were normal not only morphologically, functionally, but also in chromosomal examination. The results suggested that tubal insemination with a small number of sperms could be applied in sterility due to oligospermia in human.
authors.affiliation: Department of Veterinary Medicine, College of Agriculture and Veterinary Medicine, Nihon University, Kanagawa, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Kanayama
authors.forename: T
authors.initials: T
authors.lastname: Sankai
authors.forename: T
authors.initials: T
authors.lastname: Endo
authors.forename: Y
authors.initials: Y
authors.lastname: Sakuma
mesh_terms: D000818:Animals; D005187:Fallopian Tubes; D005260:Female; D005865:Gestational Age; D007315:Insemination, Artificial; D008098:Litter Size; D008297:Male; D010062:Ovulation Induction; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000502:physiology*; D011817:Rabbits; D013076:Sperm Count; D013094:Spermatozoa / Q000502:physiology*