title: In situ analysis of adenohypophysis proliferative activity in beagle dogs. Preliminary results.
abstract: In order to establish control data on adenohypophysis cell cycle in beagle dogs, two different approaches were used. In the first approach, the adenohypophysis mitotic index (percentage of cells in mitosis: MI) was determined using pituitary glands from 50 control beagle dogs, 10 to 25 months old, from retrospective regulatory safety assessment studies. We found that the MI in males was 0.024 +/- 0.007% and in females 0.068 +/- 0.019%. The second approach involved the determination of the adenohypophysis cell fraction in S phase by means of bromodeoxyuridine (BrdU) anti-BrdU method in 16 control beagle dogs, 8 to 20 months old, from ongoing safety assessment studies. We found that the BrdU labeling index (percentage of BrdU-positive cells per total number of cells: BrdU LI) was 0.053 +/- 0.009% in males and 0.059 +/- 0.015% in females. Analysis of our data indicated that the results obtained from the BrdU LI were more reliable (coefficient of variation [CV] less than 0.07) compared to those obtained from the MI (CV greater than 1.4). No clear difference was found between male and female beagle dogs, regarding adenohypophysis cell proliferation. In both studies, adenohypophysis cell proliferation was found to be age-dependent, with the highest value in immature dogs. No correlation between estrus cycle in females and adenohypophysis cell proliferation was noted.
authors.affiliation: Sandoz Pharma Ltd, Department of Toxicology, Basle, Switzerland.
authors.forename: M
authors.initials: M
authors.lastname: Kolopp
authors.forename: G
authors.initials: G
authors.lastname: Poirel
authors.forename: P
authors.initials: P
authors.lastname: Vit
authors.forename: E
authors.initials: E
authors.lastname: Perentes
mesh_terms: D000818:Animals; D001973:Bromodeoxyuridine / Q000378:metabolism; D002455:Cell Division / Q000502:physiology; D004247:DNA / Q000378:metabolism; D004285:Dogs / Q000502:physiology*; D004971:Estrus / Q000502:physiology; D005260:Female; D008297:Male; D008940:Mitotic Index; D010903:Pituitary Gland, Anterior / Q000166:cytology* / Q000378:metabolism / Q000502:physiology; D012189:Retrospective Studies
chemical_list: D004247:DNA; D001973:Bromodeoxyuridine