title: Exercise training has a heparin-like effect on lipoprotein lipase activity in muscle.
abstract: Lipoprotein lipase (LPL) is anchored with high affinity to heparan sulphate proteoglycans on the luminal surface of the capillary endothelium. The levels of pre-heparin perfusate LPL activity increased from 16 +/- 1 to 145 +/- 6 U/hindlimb (nine-fold increase) in hindlimb muscle of exercise-trained rats measured immediately after the last bout of work. At the same time, post-heparin perfusate LPL activity decreased from 63 +/- 2 to 13 +/- 1 U/hindlimb (p less than 0.001). These results provide evidence that exercise-training has a heparin-like effect on capillary-bound LPL. The total amount of LPL (i.e., pre-heparin perfusate plus post-heparin perfusate) was twofold greater in the hindlimb of the trained animals versus the controls. The effect of exercise on muscle LPL activity appears to last for as long as 5 days after cessation of exercise. Serum triglycerides were reduced 38% and plasma free fatty acids increased fourfold. These results provide evidence that training increases the capacity to remove triglycerides from circulation.
authors.affiliation: Department of Physical Education, University of Illinois, Chicago 60680.
authors.forename: L B
authors.initials: LB
authors.lastname: Oscai
authors.forename: R W
authors.initials: RW
authors.lastname: Tsika
authors.forename: D A
authors.initials: DA
authors.lastname: Essig
mesh_terms: D000273:Adipose Tissue / Q000166:cytology; D000818:Animals; D048429:Cell Size / Q000502:physiology; D005230:Fatty Acids, Nonesterified / Q000097:blood; D006493:Heparin / Q000502:physiology*; D008071:Lipoprotein Lipase / Q000378:metabolism*; D008297:Male; D009132:Muscles / Q000201:enzymology*; D010805:Physical Conditioning, Animal; D051381:Rats; D017208:Rats, Wistar; D014280:Triglycerides / Q000097:blood
chemical_list: D005230:Fatty Acids, Nonesterified; D014280:Triglycerides; D006493:Heparin; D008071:Lipoprotein Lipase