Loss of transcription factor AP-1 DNA binding activity during lymphocyte aging in vivo.
The main feature of cellular senescence is cessation of cell proliferation. Protooncogene c-fos, which is required for the cell to enter into DNA synthesis, is repressed in senescent fibroblasts. Diminished expression of c-fos and impaired formation of AP-1, which is a complex of c-Fos and c-Jun proteins acting as a transcription factor, was found in lymphocytes derived from old (> 18 months) mice and stimulated with Con A. There were no differences in c-jun expression and formation of other transcription factors (AP-2 and AP-3) between lymphocytes isolated from old and young mice.
Sikora
E
E
Department of Cellular Biochemistry, Nencki Institute of Experimental Biology, Warsaw, Poland.
Kamińska
B
B
Radziszewska
E
E
Kaczmarek
L
L
D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000502:physiology*; D003208:Concanavalin A / Q000494:pharmacology; D004247:DNA / Q000378:metabolism*; D008214:Lymphocytes / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D011485:Protein Binding; D016760:Proto-Oncogene Proteins c-fos / Q000235:genetics; D016755:Proto-Oncogene Proteins c-jun / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics
D016760:Proto-Oncogene Proteins c-fos; D016755:Proto-Oncogene Proteins c-jun; D012333:RNA, Messenger; D003208:Concanavalin A; D004247:DNA