Attachment of a single fluorescent label to peptides for determination by capillary zone electrophoresis.
Complicated electropherograms are produced in the separation of fluorescently labeled peptides. Incomplete labeling of epsilon-amino groups on lysine residues results in the production of 2n-1 reaction products, where n is the number of alpha and epsilon amino groups in the peptide. A single label is attached to the peptide by first taking the peptide through one cycle of the Edman degradation reaction. All epsilon-amino groups are converted to the phenyl thiocarbamyl and the cleavage step exposes one alpha-amino group at the N-terminus of the peptide; the fluorescent label is attached to the N-terminus.
Zhao
J Y
JY
Department of Chemistry, University of Alberta, Edmonton, Canada.
Waldron
K C
KC
Miller
J
J
Zhang
J Z
JZ
Harke
H
H
Dovichi
N J
NJ
D000595:Amino Acid Sequence; D004586:Electrophoresis; D005456:Fluorescent Dyes; D007202:Indicators and Reagents; D008969:Molecular Sequence Data; D010455:Peptides / Q000032:analysis*; D013050:Spectrometry, Fluorescence
D005456:Fluorescent Dyes; D007202:Indicators and Reagents; D010455:Peptides