Usage of penicillinase as an enzyme marker in enzyme linked immunosorbent assay for the detection of IgG antibodies to Toxoplasma gondii.
An Enzyme linked immunosorbent assay system has been developed using penicillinase as an enzyme marker for the detection of IgG antibodies to Toxoplasma gondii. This system is simple (end result assessed visually) reliable (the results are matching with other available commercial ELISA based kit), sensitive (detection of antitoxoplasma IgG antibodies to a level of 15 IU/ml) and reproducible (4.3 & 6.7% intra and inter coefficient of variation respectively). Experimental results with human serum samples using our system has shown consistent results and were in total agreement with other commercially available diagnostic kits. It is therefore suggested that ELISA system using penicillinase can be a simple, sensitive and reliable method for the diagnosis of toxoplasmosis in human.
Bhopale
G M
GM
Laboratory of Parasitology & Immunodiagnosis, Hindustan Antibiotics Ltd., Pune.
Naik
S R
SR
D000818:Animals; D000913:Antibodies, Protozoan / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis*; D010405:Penicillinase / Q000032:analysis*; D014122:Toxoplasma / Q000276:immunology*
D000913:Antibodies, Protozoan; D007074:Immunoglobulin G; D010405:Penicillinase