[Key points and problems concerning chemosensitivity tests, with special reference to MTT assay].
Recently, the semiautomated tetrazolium-based MTT colorimetric assay have been used to measure chemosensitivity. We also have been used this assay for 4 ovarian clear cell carcinoma cell lines to investigate the chemosensitivity of this tumor. In this study, several problems have been faced to be solved. In this paper, we pointed out these problems and indicated solutions.
Nishida
M
M
Department of Obstetrics and Gynecology, Institute of Clinical Medicine, University of Tsukuba, Japan.
Kohno
K
K
Nishide
K
K
D000230:Adenocarcinoma / Q000473:pathology; D000818:Animals; D000970:Antineoplastic Agents / Q000493:pharmacokinetics / Q000494:pharmacology; D003124:Colorimetry; D004354:Drug Screening Assays, Antitumor / Q000379:methods*; D005260:Female; D006801:Humans; D009368:Neoplasm Transplantation; D010051:Ovarian Neoplasms / Q000473:pathology; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents