The effects of thiamin on lead metabolism: organ distribution of lead 203.
The effect of thiamin on the organ distribution of lead was evaluated in CD-1 mice exposed intragastrically or intraperitoneally to a single dose of lead acetate (100 micrograms) containing 100 microCi lead 203. They were treated with either thiamin (25 or 50 mg/kg body weight), calcium ethylenediaminetetraacetic acid (CaEDTA) (50 mg/kg body weight), or combinations of thiamin and CaEDTA. The whole body retention and the organ distribution of lead 203 varied depending upon the route of lead administration, dose of thiamin and the specific treatment combination. Thiamin (25 or 50 mg/kg) treatment increased the whole body retention of both intragastric and intraperitoneal lead by approximately 10% in each instance. Calcium ethylenediaminetetraacetic acid, either alone or in combination with thiamin (50 mg/kg) reduced the whole body retention of lead by as much as 14% regardless of route of lead exposure. The relative retention of lead by the liver, kidney and spleen was greater in mice exposed to lead by the intragastric route. Regardless of route, CaEDTA in the combined treatment reduced the relative retention of lead in both the liver and kidney, whereas thiamin alone only reduced the retention of lead in the kidney. The results of this study indicate that thiamin in combination with CaEDTA alters the distribution and retention of lead in a manner which may have therapeutic application as it relates to chelation therapy.
Kim
J S
JS
Department of Veterinary Physiological Sciences, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon.
Hamilton
D L
DL
Blakley
B R
BR
Rousseaux
C G
CG
D000284:Administration, Oral; D000818:Animals; D004492:Edetic Acid / Q000494:pharmacology; D007274:Injections, Intraperitoneal; D007668:Kidney / Q000378:metabolism; D007854:Lead / Q000008:administration & dosage / Q000493:pharmacokinetics*; D008099:Liver / Q000378:metabolism; D008297:Male; D051379:Mice; D011897:Random Allocation; D013154:Spleen / Q000378:metabolism; D013831:Thiamine / Q000494:pharmacology*; D014018:Tissue Distribution / Q000187:drug effects
D007854:Lead; D004492:Edetic Acid; D013831:Thiamine