Influence of HDL on the formation of 6-keto-PGF1 alpha and TXB2 in vitro: the importance of the source of HDL.
The influence of HDL, isolated from normolipidemic human blood and blood of normo- and hyperlipidemic rabbits, on in vitro 6-keto-PGF1 alpha synthesis by rabbit aorta and on TXB2 synthesis by platelets of clotting human and rabbit blood was tested. The HDL fraction from normolipidemic subjects, when incubated with blood from normolipidemic humans or rabbits, inhibited TXB2 formation. The same fraction stimulated the formation of 6-keto-PGF1 alpha after incubation with rabbit aorta taken from normolipidemic animals. HDL taken from hyperlipidemic rabbits inhibited 6-keto-PGF1 alpha formation in rabbits and had no influence on TXB2 formation. These results support the hypothesis that not only is the absolute amount of HDL important for its influence on prostanoid formation, but also the origin and the composition of the HDL fraction.
Beitz
J
J
Department of Pharmacology and Toxicology, Martin Luther University Halle-Wittenberg, Halle, Federal Republic of Germany.
Beitz
A
A
Müller
G
G
Giessler
C
C
Mest
H J
HJ
D015121:6-Ketoprostaglandin F1 alpha / Q000096:biosynthesis*; D000818:Animals; D001011:Aorta / Q000378:metabolism*; D001777:Blood Coagulation / Q000502:physiology*; D001792:Blood Platelets / Q000378:metabolism; D006801:Humans; D006949:Hyperlipidemias / Q000097:blood; D066298:In Vitro Techniques; D008075:Lipoproteins, HDL / Q000502:physiology*; D011817:Rabbits; D013045:Species Specificity; D013929:Thromboxane B2 / Q000096:biosynthesis*
D008075:Lipoproteins, HDL; D013929:Thromboxane B2; D015121:6-Ketoprostaglandin F1 alpha