Secretion of alpha-hemolysin by bovine mammary isolates of Staphylococcus aureus.
A total of 262 strains of Staphylococcus aureus isolated from the mammary gland of dairy cows were examined for the production of alpha-hemolysin. Strains were cultured in a liquid medium of casein hydrolysate and yeast extract in an atmosphere of 7% (v/v) CO2 in air. The assay consisted of a dot immunoblotting technique employing bacterial culture supernatants and a mouse monoclonal antibody specific for alpha-hemolysin. Ninety-four percent (247) of 262 strains were positive for alpha-hemolysin by this method, when cultured in the laboratory. This figure is compared with those obtained in previous studies which typically based their results on the hemolytic patterns of isolates on blood agar plates.
Kenny
K
K
Department of Clinical Sciences, New York State College of Veterinary Medicine, Cornell University, Ithaca 14853.
Bastida
F D
FD
Norcross
N L
NL
D000818:Animals; D001427:Bacterial Toxins / Q000096:biosynthesis*; D002417:Cattle; D005260:Female; D006460:Hemolysin Proteins / Q000096:biosynthesis*; D006825:Hybridomas; D008321:Mammary Glands, Animal / Q000382:microbiology*; D008414:Mastitis, Bovine / Q000382:microbiology; D008892:Milk / Q000382:microbiology; D013203:Staphylococcal Infections / Q000382:microbiology / Q000662:veterinary; D013211:Staphylococcus aureus / Q000378:metabolism* / Q000472:pathogenicity; D014774:Virulence
D001427:Bacterial Toxins; D006460:Hemolysin Proteins; C021091:staphylococcal alpha-toxin