Mouse hepatic microsomal oxidation of aliphatic aldehydes (C8 to C11) to carboxylic acids.
Addition of saturated and alpha, beta-unsaturated aliphatic aldehydes (C8 to C11) significantly increased NADPH oxidation with mouse hepatic microsomes, and the aldehydes themselves were oxidized to the corresponding carboxylic acids. When these aldehyde substrates were incubated similarly under oxygen-18 gas and the carboxylic acids formed were analyzed by GC-MS after methylation, it was indicated that oxygen-18 was significantly incorporated into the carboxylic acids formed from alpha, beta-unsaturated aldehydes, but not significantly into the carboxylic acids formed from saturated aldehydes. These results indicate that enzyme and/or mechanism responsible for the oxidation of these two types of aldehydes is different from each other.
Watanabe
K
K
Department of Hygienic Chemistry, Faculty of Pharmaceutical Sciences, Hokuriku University, Kanazawa, Japan.
Matsunaga
T
T
Narimatsu
S
S
Yamamoto
I
I
Yoshimura
H
H
D000438:Alcohols / Q000378:metabolism*; D000447:Aldehydes / Q000378:metabolism*; D000818:Animals; D008401:Gas Chromatography-Mass Spectrometry; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D008862:Microsomes, Liver / Q000378:metabolism*; D009243:NAD / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D010084:Oxidation-Reduction
D000438:Alcohols; D000447:Aldehydes; D009243:NAD; D009249:NADP