title: Expression of the bcl-2 oncogene product and chromosomal translocation t(14;18) in Hodgkin's disease.
abstract: The B-cell lymphoma/leukemia oncogene bcl-2 takes part in crucial regulatory events in B-cell maturation and differentiation. The reciprocal chromosomal translocation t(14;18), leading to overexpression of this oncogene, can be found in the majority of follicular lymphomas and much less frequently in B-cell leukemias and diffuse lymphomas. We have studied the expression of this protein in different subtypes of Hodgkin's disease using monoclonal antibodies directed against a formalin-resistant epitope of the bcl-2 protein and also have investigated these cases by polymerase chain reaction for evidence of the t(14;18) translocation. We were particularly interested to determine whether nodular paragranuloma (lymphocyte-predominant, nodular), which differs from other subtypes of Hodgkin's disease by virtue of the B-cell nature of its malignant cell population, is characterized by expression of the bcl-2 protein. Our data indicate that only a small number of nodular paragranulomas express the bcl-2 protein and that the expression is not specific for this type of Hodgkin's disease. In a smaller number of cases this expression of bcl-2 could be explained by the presence of the translocation t(14;18).
authors.affiliation: Institute of Pathology, University of Cologne, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Lorenzen
authors.forename: M L
authors.initials: ML
authors.lastname: Hansmann
authors.forename: F
authors.initials: F
authors.lastname: Pezzella
authors.forename: C
authors.initials: C
authors.lastname: Hesse
authors.forename: M
authors.initials: M
authors.lastname: Kneba
authors.forename: K C
authors.initials: KC
authors.lastname: Gatter
authors.forename: R
authors.initials: R
authors.lastname: Fischer
mesh_terms: D000911:Antibodies, Monoclonal; D002883:Chromosomes, Human, Pair 14; D002887:Chromosomes, Human, Pair 18; D015870:Gene Expression; D006689:Hodgkin Disease / Q000235:genetics* / Q000473:pathology*; D006801:Humans; D007124:Immunoenzyme Techniques; D016133:Polymerase Chain Reaction; D011518:Proto-Oncogene Proteins / Q000032:analysis*; D019253:Proto-Oncogene Proteins c-bcl-2; D016539:Reed-Sternberg Cells / Q000473:pathology; D014178:Translocation, Genetic
chemical_list: D000911:Antibodies, Monoclonal; D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2