title: Utrophin, the autosomal homologue of dystrophin, is widely-expressed and membrane-associated in cultured cell lines.
abstract: Utrophin, the autosomal dystrophin-related protein (DRP), is expressed in HeLa cells, smooth muscle-like BC3H1 cells from mouse brain, COS monkey kidney cells, the P388D1 monocyte-macrophage cell line and untransformed human skin fibroblasts, as well as in rat C6 glioma and Schwannoma cells. It was undetectable, however, in the Sp2/O mouse myeloma cell line and in hybridoma lines derived from it. Dystrophin was not detected in any of these cell lines. Although all utrophin-containing cells were capable of forming monolayers in culture, no major effects of either attachment to substratum or length of time in culture (2-17 days) on utrophin levels were observed. After subcellular fractionation of BC3H1 or glioma cells, nearly all of the utrophin was found in the Triton-soluble fraction, suggesting an association with cell membranes.
authors.affiliation: Research Division, N.E. Wales Institute, Deeside, Clwyd, UK.
authors.forename: T M
authors.initials: TM
authors.lastname: Nguyen
authors.forename: T T
authors.initials: TT
authors.lastname: Le
authors.forename: D J
authors.initials: DJ
authors.lastname: Blake
authors.forename: K E
authors.initials: KE
authors.lastname: Davies
authors.forename: G E
authors.initials: GE
authors.lastname: Morris
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002458:Cell Fractionation; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D003598:Cytoskeletal Proteins / Q000096:biosynthesis*; D006367:HeLa Cells; D006801:Humans; D008565:Membrane Proteins / Q000378:metabolism*; D051379:Mice; D051381:Rats; D049411:Utrophin
chemical_list: D003598:Cytoskeletal Proteins; D008565:Membrane Proteins; C483600:Utrn protein, mouse; D049411:Utrophin