title: Maturation and responsiveness to extracts of corpora allata from male Locusta migratoria containing allatotropic factors.
abstract: Corpora allata (CA) from male Locusta migratoria were tested for their ability to synthesize juvenile hormone (JH) and to respond to stimulating brain/corpora cardiaca (CC)-extracts under in vitro conditions. We found that a preincubation of the CA of both sexes at 4 degrees C for 24 h lowers their basal rate of synthesis and retains their responsiveness to allatotropic factors. Male CA can be stimulated by brain/CC extracts as well as female CA. JH biosynthesis stimulating factors are also present in male brain/CC extracts. Thus such extracts from male locusts can be used for the isolation of locust allatotropin. Furthermore male locust CA are appropriate for bioassaying such allatotropic factors.
authors.affiliation: Department of Entomology, Faculty of Agriculture, Hebrew University, Rehovot, Israel.
authors.forename: E
authors.initials: E
authors.lastname: Lehmberg
authors.forename: H J
authors.initials: HJ
authors.lastname: Ferenz
authors.forename: S W
authors.initials: SW
authors.lastname: Applebaum
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D005260:Female; D006110:Grasshoppers / Q000187:drug effects / Q000502:physiology*; D007605:Juvenile Hormones / Q000096:biosynthesis*; D007700:Kinetics; D008297:Male; D008715:Methionine / Q000378:metabolism; D009424:Nervous System Physiological Phenomena; D012727:Sex Characteristics; D014020:Tissue Extracts / Q000494:pharmacology*
chemical_list: D007605:Juvenile Hormones; D014020:Tissue Extracts; D008715:Methionine