Physical mapping of the albino-deletion complex in the mouse to localize alf/hsdr-1, a locus required for neonatal survival.
The albino-deletion complex in the mouse defines a genetically well-characterized region of chromosome 7 in which a number of loci essential for normal development and viability reside. One locus, designated alf or hsdr-1, is necessary for neonatal survival. Its absence results in hypoglycemia associated with biochemical and ultrastructural abnormalities in hepatocytes and proximal tubule cells of the kidney. We constructed a long-range physical map of the region defined by the proximal segment of the albino-deletion complex as a step toward localizing alf/hsdr-1. Sixteen markers, including 11 whose isolation is described here and in the accompanying paper (A. Schedl et al., 1992, Genomics 14, 288-297), were ordered on a panel of albino-deletion DNAs and their distribution was examined by pulsed-field gel electrophoresis. The resulting approximately 4300-kb physical map covers the entire region absent from the prototypic alf/hsdr-1 deletion c14CoS, estimated as approximately 3600 kb. Since the deletion c11DSD complements and overlaps most of c14CoS, alf/hsdr-1 was mapped at the proximal extreme of c14CoS, approximately 3000 kb from the albino locus. The density of CpG islands was found to be very heterogeneous across the region mapped.
Kelsey
G
G
Institute of Cell and Tumor Biology, German Cancer Research Center, Heidelberg.
Schedl
A
A
Ruppert
S
S
Niswander
L
L
Magnuson
T
T
Klebig
M L
ML
Rinchik
E M
EM
Schütz
G
G
D000417:Albinism / Q000235:genetics*; D000818:Animals; D000831:Animals, Newborn; D015139:Blotting, Southern; D002874:Chromosome Mapping; D015342:DNA Probes; D016521:Electrophoresis, Gel, Pulsed-Field; D017353:Gene Deletion; D005819:Genetic Markers; D007687:Kidney Tubules, Proximal / Q000002:abnormalities; D008099:Liver / Q000002:abnormalities / Q000166:cytology; D051379:Mice; D015183:Restriction Mapping
D015342:DNA Probes; D005819:Genetic Markers