Separation of glucuronides from urine by coupled-column separation using underivatized silica as precolumn.
Glucuronides are separated from urine by coupled-column separations (CCSs). The fraction containing the glucuronide(s) is transferred on-line from a silica precolumn to the analytical column (octadecyl derivatized silica), enriched, and separated by ion-pair chromatography. The retention and selectivity on the precolumn are controlled by pH, buffer components, organic modifier, and ion-pair agent. After the injection of filtered urine samples, glucuronides with different chemical properties can be separated. The total analysis of morphine-3-glucuronide and morphine-6-glucuronide is accomplished in less than six minutes, with UV detection at 210 nm.
Stefansson
M
M
Department of Analytical Pharmaceutical Chemistry, Uppsala University, Sweden.
Lu
B
B
Westerlund
D
D
D002021:Buffers; D000077286:Cetrimonium; D002593:Cetrimonium Compounds / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D005965:Glucuronates / Q000652:urine*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D013056:Spectrophotometry, Ultraviolet
D002021:Buffers; D002593:Cetrimonium Compounds; D005965:Glucuronates; D000077286:Cetrimonium