The kidney in the fetal rat model of congenital diaphragmatic hernia induced by nitrofen.
This paper explores whether there is a correlation between kidney and lung growths in an experimental model of congenital diaphragmatic hernia (CDH) induced by intragastric administration of Nitrofen (115 mg/kg) in olive oil on time-dated pregnant Wistar rats at the 9th day of gestation. For comparison we used pregnant rats treated with olive oil alone. Twenty-nine normal fetuses from 3 control rats and 24 left CDH fetuses from 6 Nitrofen rats were studied. Fetal (3.6 +/- 0.8 v 4.9 +/- 0.4 g, P < .001) and total lung (2% +/- 0.5% v 2.6% +/- 0.3% of body weight, P < .001) weights were significantly decreased in animals with CDH. Kidneys were also smaller in CDH animals although not significantly (0.7% +/- 0.1% v 0.8% +/- 0.1% of body weight, P = .05) and were also histologically immature. Regression of kidney weight on body weight for both groups yielded regression lines that were identical at analysis of covariance and all data points from the CDH group were within the control group 95% confidence limits. After converting raw data into lung/body and kidney/body weight ratios, no inverse correlation suggesting a feedback mechanism of growth regulation between both organs could be found. Since nitrofen acts through modifications of the thyroid hormone status in both dam and fetus, altered maturation of several organs should be expected although some of them, like the lung, are the leading targets. The present CDH rodent model is probably different from the human malformation in spite of the striking anatomic similarities between them.(ABSTRACT TRUNCATED AT 250 WORDS)
Tovar
J A
JA
Universidad del País Vasco, Hospital NaSa de Aranzazu, San Sebastián, Spain.
Alfonso
L F
LF
Aldazabal
P
P
Lopez de Torre
B
B
Uriarte
S
S
Vilanova
J
J
D000014:Abnormalities, Drug-Induced / Q000196:embryology* / Q000473:pathology; D000818:Animals; D003964:Diaphragm / Q000473:pathology; D004195:Disease Models, Animal; D005260:Female; D006540:Herbicides / Q000633:toxicity*; D006548:Hernia, Diaphragmatic / Q000139:chemically induced* / Q000196:embryology / Q000473:pathology; D007668:Kidney / Q000187:drug effects* / Q000196:embryology / Q000473:pathology; D008168:Lung / Q000187:drug effects / Q000196:embryology / Q000473:pathology; D010647:Phenyl Ethers / Q000633:toxicity*; D011247:Pregnancy; D051381:Rats
D006540:Herbicides; D010647:Phenyl Ethers; C007350:nitrofen