title: A rapid and reliable method to create tandem arrays of short DNA sequences.
abstract: Tandemly polymerized regulatory elements, antisense RNA segments or ribozymes are potentially useful in selective gene silencing. However, existing methods of tandemly polymerizing short DNA segments are laborious. We present a procedure that can create cloned arrays of 40-70 monomer units in two steps. We have created long arrays of regulatory elements and potential ribozyme sequences. Silencing of human immunodeficiency virus (HIV-1) activation by tandem arrays of a regulatory element in human immune system cells and in other human and monkey cells is discussed.
authors.affiliation: Department of Cell Biology, Albert Einstein College of Medicine, Bronx, NY 10461.
authors.forename: G J
authors.initials: GJ
authors.lastname: Graham
authors.forename: J J
authors.initials: JJ
authors.lastname: Maio
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000138:chemical synthesis; D004256:DNA Polymerase I; D004926:Escherichia coli; D008969:Molecular Sequence Data; D010957:Plasmids; D011108:Polymers; D012045:Regulatory Sequences, Nucleic Acid; D012091:Repetitive Sequences, Nucleic Acid; D012270:Ribosomes
chemical_list: D011108:Polymers; D004247:DNA; D004256:DNA Polymerase I