Modification of the effects of hyperthermia and neutron radiation on the activity of acid phosphatase in CaNT tumors.
Acid phosphatase activity was measured in implanted murine CaNT tumors of varying volumes. There is a clear monotonically increasing relation between acid phosphatase activity and tumor volume. Also the tumors were subjected to either induced artificial hypoxia or hyperthermia (41.0 degrees C) alone, or combined with neutron irradiation (3.8 Gy). Changes in the activity of this enzyme following radiation damage could reflect tissue damage associated with metabolic disturbances. The effect on enzyme activity after sequential hyperthermia and neutron irradiation is not synergistic, as is shown in the quantitative experimental data. This implies that the mechanisms of heat damage differ from that of neutron beam damage, as reflected by acid phosphatase activity. The CaNT tumor was also shown to be thermosensitive after administration of mitoxantrone. Finally, the role of exogenous ATP was shown to provide heat protection by modification of those thermal effects resulting in the activity of acid phosphatase. The augmentation of this hydrolytic enzyme probably represents initial metabolic damage in the tumor after different modalities of radiation alone, or combined with mitoxantrone and exogenous ATP.
Szeinfeld
D
D
Research Institute for Medical Biophysics, Medical Research Council, Tygerberg, South Africa.
de Villiers
N
N
Wynchank
S
S
D000135:Acid Phosphatase / Q000378:metabolism* / Q000528:radiation effects; D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D003131:Combined Modality Therapy; D006979:Hyperthermia, Induced; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D008942:Mitoxantrone / Q000494:pharmacology; D009374:Neoplasms, Experimental / Q000201:enzymology* / Q000628:therapy; D009502:Neutrons
D000255:Adenosine Triphosphate; D008942:Mitoxantrone; D000135:Acid Phosphatase