Effect of serum on adrenal PNMT activity.
Humoral mechanism should be responsible for activation of PNMT (phenylethanolamine N-methyltransferase) by CRF (corticotropin-releasing factor) in vivo (Lima and Sourkes, 1987). Small amounts of serum (10 microliters) caused a sig. dose dependent activation of bovine PNMT activity (19.0 +/- 1.8%) changing in Km and Vmax values in vitro. Human/rat or ovine CRF--in high amounts (8.4 nmol) only--increase at the rate of just 10% bovine PNMT activity in vitro. At the moment, we do not know which factor in serum is responsible for the increase in A (adrenalin) synthesis in vitro.
Matussek
N
N
Department of Neurochemistry, Psychiatric Hospital, University of Munich, Federal Republic of Germany.
Deuringer
L
L
Sardas-Trevorrow
I
I
D000311:Adrenal Glands / Q000201:enzymology*; D000328:Adult; D000368:Aged; D000818:Animals; D002417:Cattle; D003346:Corticotropin-Releasing Hormone / Q000494:pharmacology; D003866:Depressive Disorder / Q000097:blood*; D005260:Female; D006801:Humans; D007546:Isoquinolines / Q000494:pharmacology; D007700:Kinetics; D008297:Male; D008875:Middle Aged; D010625:Phenylethanolamine N-Methyltransferase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D044005:Tetrahydroisoquinolines
D007546:Isoquinolines; D044005:Tetrahydroisoquinolines; D003346:Corticotropin-Releasing Hormone; C012153:7,8-dichloro-1,2,3,4-tetrahydroisoquinoline; D010625:Phenylethanolamine N-Methyltransferase