Ultrastructural effect of L-ascorbic acid 2-phosphate on cultured keratocytes.
I studied the effect of L-ascorbic acid 2-phosphate (P-Asc), a long-acting derivative of L-ascorbic acid, on the fine structures of cultured rabbit keratocytes. The results showed that cells cultured with 0.1 mM P-Asc for 30 days were more markedly multilayered than those grown without P-Asc. Dilation of the cisternae of the endoplasmic reticulum in the control cells indicated an accumulation of protein (probably procollagen). In addition, many lysosome-like structures, which may degrade underhydroxylated procollagen, were observed in the cytoplasm of the control cells. A slight increment of free ribosomes was also found within the control cells. P-Asc enhanced the multilayerization of cultured keratocytes. We conclude that the changes seen in the cytoplasm are due to the effect of P-Asc acting as vitamin C.
Saika
S
S
Department of Ophthalmology, Wakayama Medical College, Japan.
D000818:Animals; D001205:Ascorbic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D002478:Cells, Cultured; D003238:Connective Tissue / Q000187:drug effects / Q000648:ultrastructure; D003319:Corneal Stroma / Q000187:drug effects* / Q000648:ultrastructure*; D005109:Extracellular Matrix / Q000187:drug effects / Q000648:ultrastructure; D008297:Male; D015388:Organelles / Q000187:drug effects / Q000648:ultrastructure; D011817:Rabbits
C011669:ascorbate-2-phosphate; D001205:Ascorbic Acid