Cloning and 5'-flanking sequence of a rat cholesterol 7 alpha-hydroxylase gene.
A cholesterol 7 alpha-hydroxylase gene containing 8 kb of the 5'-flanking region and 5 kb of the transcription region which covers exons 1 to 5 was isolated from a rat genomic library. The 2015 bp nucleotide sequence 5'-upstream from the start codon was determined. This promotor region contains many liver-enriched or -specific elements (TGT3, HNF/LF-B1), putative hormone responsive elements (TRE, GRE, RRE or RARE) and ubiquitous transcription factor binding sites (NF-1, OCT-1). In addition, 21 CA repeats which have potential to form the Z-DNA structure were found in this region. These putative regulatory elements and repetitive motifs may play roles in the regulation of cholesterol 7 alpha-hydroxylase gene in the liver. The sequence identity of the rat gene to the human gene in this region is low. Only liver-enriched elements are conserved in the cholesterol 7 alpha-hydroxylase genes.
Chiang
J Y
JY
Department of Biochemistry and Molecular Pathology, Northeastern Ohio Universities College of Medicine, Rootstown 44272.
Yang
T P
TP
Wang
D P
DP
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002790:Cholesterol 7-alpha-Hydroxylase / Q000235:genetics*; D003001:Cloning, Molecular; D004247:DNA; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D051381:Rats; D015183:Restriction Mapping
D004247:DNA; D002790:Cholesterol 7-alpha-Hydroxylase