Discrimination between alpha-satellite DNA sequences from chromosomes 21 and 13 by using polymerase chain reaction.
alpha-Satellite subfamilies from chromosomes 21 and 13 are almost identical in sequence and cannot be distinguished from each other by hybridization techniques. A general method based on membrane-bound PCR is described here, allowing the discrimination of alpha-satellite DNA sequences from each of these two chromosomes, after detection by Southern blot hybridization. The PCR conditions were developed using somatic hybrid DNAs. The method was tested in membrane-bound PCR by using the alpha-satellite bands from a Southern blot of a CEPH family. The chromosomal origin of these bands, previously determined by linkage analysis, was confirmed by this method.
Charlieu
J P
JP
CNRS UPR 9008, Montpellier, France.
Murgue
B
B
Laurent
A M
AM
Marçais
B
B
Bellis
M
M
Roizès
G
G
D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002882:Chromosomes, Human, Pair 13; D002891:Chromosomes, Human, Pair 21; D004276:DNA, Satellite / Q000235:genetics*; D004277:DNA, Single-Stranded; D006801:Humans; D006822:Hybrid Cells; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction
D004276:DNA, Satellite; D004277:DNA, Single-Stranded