Evaluation of the anaerobic method for the analysis of fecal microflora of beagle dogs.
The plate-in-bottle method of Mitsuoka et al. (1969) for the counting of fecal bacteria in beagle dogs was superior to an anaerobic jar method. Comparisons of three nonselective media, such as medium 10 supplemented with 10% cecal extract of dogs (designated as M10C), M10 with 10% fecal extract of dogs (M10F), and M10, by the plate-in-bottle method indicated that the visible bacterial counts for M10C were higher than those for M10 and M10F. The high percentage (18.4%) of numbers of the extremely oxygen-sensitive anaerobes to the fecal total counts by using the plate-in-bottle method with M10C was also observed.
Benno
Y
Y
Japan Collection of Microorganisms, Institute of Physical and Chemical Research RIKEN, Saitama.
Mitsuoka
T
T
D000818:Animals; D001421:Bacteria, Anaerobic / Q000302:isolation & purification*; D001431:Bacteriological Techniques; D004285:Dogs / Q000382:microbiology*; D005069:Evaluation Studies as Topic; D005243:Feces / Q000382:microbiology*; D008297:Male