title: Stimulation of polydnavirus replication by 20-hydroxyecdysone.
abstract: During oviposition the endoparasitic wasp Campoletis sonorensis, introduces a polydnavirus into parasitized insects where viral gene expression is required for endoparasite survival. The polydnavirus is integrated into wasp chromosomal DNA and replicates only in the ovary. Ecdysteroids regulate the developmental expression of many insect genes and may regulate polydnavirus replication. Direct verification of viral replication was performed by dot blot hybridization and by amplifying DNA sequences containing the viral integration site; this 'junction' fragment cannot be amplified from integrated virus. Thoracic ligations and in vitro ecdysteroid treatments of wasp ovaries support the hypothesis that polydnavirus DNA replication is regulated by ecdysteroid during parasite development.
authors.affiliation: Department of Entomology, Texas A&M University, College Station 77843.
authors.forename: B A
authors.initials: BA
authors.lastname: Webb
authors.forename: M D
authors.initials: MD
authors.lastname: Summers
mesh_terms: D000818:Animals; D001483:Base Sequence; D004261:DNA Replication / Q000187:drug effects*; D004278:DNA, Superhelical / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification; D004441:Ecdysterone / Q000494:pharmacology*; D005260:Female; D006927:Hymenoptera; D007304:Insect Viruses / Q000187:drug effects* / Q000235:genetics / Q000502:physiology; D007915:Lepidoptera; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D016133:Polymerase Chain Reaction; D011679:Pupa; D015183:Restriction Mapping; D016662:Virus Integration; D014779:Virus Replication / Q000187:drug effects*
chemical_list: D004278:DNA, Superhelical; D004279:DNA, Viral; D009838:Oligodeoxyribonucleotides; D004441:Ecdysterone