Effect of LY 83583 on relaxation induced by non-adrenergic non-cholinergic nerve stimulation and exogenous nitric oxide in the rat gastric fundus.
In the rat gastric fundus, the reported cGMP-lowering agent LY 83583 (10(-5) M) inhibited the relaxation induced by nitric oxide (NO), without altering the response to isoprenaline, vasoactive intestinal polypeptide, sodium nitroprusside or electrical field stimulation of inhibitory non-adrenergic non-cholinergic neurones, which are thought to release NO. Incubation with superoxide dismutase partially prevented the effect of LY 83583. When added during a relaxation maintained by continuous NO infusion, LY 83583 reversed the relaxation. It is concluded that LY 83583 inactivates exogenous NO through the generation of superoxide anions.
Barbier
A J
AJ
Heymans Institute of Pharmacology, University of Gent Medical School, Belgium.
Lefebvre
R A
RA
D000634:Aminoquinolines / Q000494:pharmacology*; D000818:Animals; D004558:Electric Stimulation; D005260:Female; D005748:Gastric Fundus / Q000187:drug effects* / Q000294:innervation; D008297:Male; D009126:Muscle Relaxation / Q000187:drug effects; D009130:Muscle, Smooth / Q000187:drug effects*; D009569:Nitric Oxide / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D013189:SRS-A / Q000037:antagonists & inhibitors*; D013564:Sympathetic Nervous System / Q000502:physiology*
D000634:Aminoquinolines; D013189:SRS-A; D009569:Nitric Oxide; C041715:6-anilino-5,8-quinolinedione