title: Species of Pseudomonas obtained at 7 degrees C and 30 degrees C during aerobic storage of lamb carcasses.
abstract: A total of 268 strains of Pseudomonas isolated during storage life of lamb carcasses was identified to species level. One-hundred and thirteen strains obtained at 30 degrees C were Ps. fragi (51), Ps. lundensis (17), Ps. fluorescens biovars I (10), III (9) and VI (1), Ps. putida biovar A (8 strains) and unidentified (17 strains). Species and biovars isolated at 7 degrees C (155) were Ps. fragi (101), Ps. lundensis (32), Ps. fluorescens biovar I (6), Ps. putida biovar A (8) and unidentified (8). Numerical analysis (82% SSM, UPGMA) of 'psychrotrophic' and 'mesophilic' strains resulted in the formation of nine and eight clusters respectively. The dendrograms obtained exhibited similar structures. Most of the strains of Ps. lundensis and Ps. fragi clustered together. Strains of this latter species also joined the type strain of Ps. testosteroni and appeared included with phenons containing the Ps. putida strains. There were clusters made up exclusively of strains assigned to one biovar or group (Ps. fluorescens biovars I and II and unidentified). A high level of similarity was observed between clusters of Ps. fluorescens biovar I and those containing the Ps. fragi-Ps. lundensis complex (> 74% SSM) and Ps. lundensis (> 80%). The recovery of pseudomonads seemed to be affected by the sampling day.
authors.affiliation: Department of Food Hygiene and Food Technology, University of León, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Prieto
authors.forename: M R
authors.initials: MR
authors.lastname: García-Armesto
authors.forename: M L
authors.initials: ML
authors.lastname: García-López
authors.forename: C
authors.initials: C
authors.lastname: Alonso
authors.forename: A
authors.initials: A
authors.lastname: Otero
mesh_terms: D000332:Aerobiosis; D000818:Animals; D003080:Cold Temperature; D005516:Food Microbiology; D006358:Hot Temperature; D006801:Humans; D008460:Meat / Q000382:microbiology*; D011549:Pseudomonas / Q000145:classification / Q000302:isolation & purification*; D012756:Sheep / Q000382:microbiology*