title: A streptavidin-biotin-enhanced nitrocellulose enzyme immunoassay for the detection of rhabdovirus of penaeid shrimps from infected animals.
abstract: A streptavidin-biotin-enhanced nitrocellulose enzyme immunoassay was developed for the detection of the rhabdovirus of penaeid shrimps (RPS) in the tissues of infected animals. Initial tests indicate that the assay was capable of detecting as few as ten plaque-forming units of virus.
authors.affiliation: Department of Microbiology, University of Hawaii, Honolulu.
authors.forename: E C
authors.initials: EC
authors.lastname: Nadala
authors.forename: Y
authors.initials: Y
authors.lastname: Lu
authors.forename: P C
authors.initials: PC
authors.lastname: Loh
authors.forename: J A
authors.initials: JA
authors.lastname: Brock
mesh_terms: D000818:Animals; D001426:Bacterial Proteins; D001710:Biotin; D003101:Collodion; D033364:Decapoda / Q000382:microbiology*; D005069:Evaluation Studies as Topic; D007124:Immunoenzyme Techniques; D012209:Rhabdoviridae / Q000302:isolation & purification*; D019809:Streptavidin; D010948:Viral Plaque Assay / Q000379:methods; D014773:Virology / Q000379:methods
chemical_list: D001426:Bacterial Proteins; D001710:Biotin; D003101:Collodion; D019809:Streptavidin