title: New trypsin inhibitors are present in the coelomic fluid of the earthworm Lumbricus terrestris.
abstract: Proteinase-inhibiting components of the coelomic fluid of the earthworm Lumbricus terrestris were examined. Inhibition of proteinases of serine, aspartate and thiol families was tested. Very strong inhibition was observed only in the case of trypsin. Additional data suggest that the inhibition is related to proteins of molar mass of 42 kDa and 20 kDa, respectively. These two proteins are present in the coelomic fluid in several forms which differ in their isoelectric points.
authors.affiliation: Institute of Organic Chemistry and Biochemistry, Czechoslovak Academy of Sciences, Prague, CSFR.
authors.forename: Z
authors.initials: Z
authors.lastname: Voburka
authors.forename: M
authors.initials: M
authors.lastname: Mares
authors.forename: V
authors.initials: V
authors.lastname: Vĕtvicka
authors.forename: M
authors.initials: M
authors.lastname: Bilej
authors.forename: M
authors.initials: M
authors.lastname: Baudys
authors.forename: M
authors.initials: M
authors.lastname: Fusek
mesh_terms: D000818:Animals; D016282:Aspartic Acid Endopeptidases / Q000378:metabolism; D015153:Blotting, Western; D005591:Chemical Fractionation; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D003546:Cysteine Endopeptidases / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D008970:Molecular Weight; D009835:Oligochaeta / Q000737:chemistry*; D012697:Serine Endopeptidases / Q000378:metabolism; D014361:Trypsin Inhibitors / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology
chemical_list: D014361:Trypsin Inhibitors; D012697:Serine Endopeptidases; D003546:Cysteine Endopeptidases; D016282:Aspartic Acid Endopeptidases