Determination of vasoactive intestinal peptide in rat brain by high-performance capillary electrophoresis.
A high-performance capillary electrophoresis (HPCE) method for determining vasoactive intestinal peptide (VIP) in rat brain was developed. Cerebral cortex was first extracted by solid-phase extraction and purified by reversed-phase high-performance liquid chromatography. The VIP-rich fraction was further analysed by capillary zone electrophoresis (CZE) and micellar electrokinetic chromatography using a commercial HPCE instrument with UV detection. The identity of the peak of endogenous VIP was confirmed by performing multiple CZE analyses at different pH values. This HPCE method allows VIP to be detected and measured with good molecular specificity and could represent a reference method to validate data obtained by radioimmunoassay.
Soucheleau
J
J
Département de Médecine Expérimentale, INSERM U52-CNRS URA 1195, Université Claude Bernard, Lyon, France.
Denoroy
L
L
D000818:Animals; D001923:Brain Chemistry; D002540:Cerebral Cortex / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D004586:Electrophoresis; D006863:Hydrogen-Ion Concentration; D008297:Male; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains; D014660:Vasoactive Intestinal Peptide / Q000032:analysis*
D014660:Vasoactive Intestinal Peptide