title: Molecular images of cereal proteins by STM.
abstract: Scanning tunnelling microscopy (STM) has been used to study a seed storage protein of wheat known as gamma-gliadin. The protein was deposited onto highly oriented pyrolytic graphite (HOPG) from solutions of trifluoroethanol (TFE) and 1% acetic acid. Samples were dried down and then scanned in air. Transmission electron microscopy (TEM) was also used to visualise the distribution of protein on the substrate. Small-angle X-ray scattering (SAXS) was used to compare the molecular size and shape obtained with those from the STM images.
authors.affiliation: H.H. Wills Physics Laboratory, University of Bristol, UK.
authors.forename: N H
authors.initials: NH
authors.lastname: Thomson
authors.forename: M J
authors.initials: MJ
authors.lastname: Miles
authors.forename: A S
authors.initials: AS
authors.lastname: Tatham
authors.forename: P R
authors.initials: PR
authors.lastname: Shewry
mesh_terms: D005903:Gliadin / Q000648:ultrastructure*; D008854:Microscopy, Electron; D016252:Microscopy, Scanning Tunneling
chemical_list: D005903:Gliadin