Isolation of lactose-binding lectins from axolotl (Ambystoma mexicanum).
1. Lactose-inhibitable hemagglutination activity was identified in extracts of axolotl (Ambystoma mexicanum) larvae. 2. Two types of lectin were isolated from extracts by affinity chromatography on lactose-Sepharose. 3. A thiol-independent lectin of subunit mol. wt 15 kDa and a thiol-dependent lectin of subunit mol. wt 18 kDa were identified. 4. The 15 kDa and a 18 kDa polypeptides were weakly reactive with polyclonal anti-human galaptin serum.
Allen
H J
HJ
Department of Gynecologic Oncology, Roswell Park Cancer Institute, Buffalo, New York 14263.
Ahmed
H
H
Sharma
A
A
D000557:Ambystoma / Q000378:metabolism*; D000818:Animals; D037541:Galectin 4; D037161:Galectins; D006388:Hemagglutinins / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*; D007120:Immunochemistry; D037102:Lectins / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*; D008970:Molecular Weight; D011487:Protein Conformation
D037541:Galectin 4; D037161:Galectins; D006388:Hemagglutinins; D037102:Lectins