Determination of sterols, erythrodiol, uvaol and alkanols in olive oils using combined solid-phase extraction, high-performance liquid chromatographic and high-resolution gas chromatographic techniques.
A method is described for the determination of the sterol, erythrodiol, uvaol and alkanol content in olive oils by means of solid-phase extraction and high-performance liquid chromatography, instead of liquid-liquid and thin-layer chromatographic separations, the following step being high-resolution gas chromatographic separation. This type of procedure allows the simultaneous analysis of a larger number of samples and a substantial reduction in manual operations. Comparisons were made between the two methods on 100 different olive oils and with a statistical analysis of the results (Student's t-test).
Amelio
M
M
Fratelli Carli SpA, Imperia, Italy.
Rizzo
R
R
Varazini
F
F
D002849:Chromatography, Gas / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005233:Fatty Alcohols / Q000032:analysis*; D009828:Oleanolic Acid / Q000031:analogs & derivatives* / Q000032:analysis; D000069463:Olive Oil; D010938:Plant Oils / Q000032:analysis*; D013261:Sterols / Q000032:analysis*; D014315:Triterpenes / Q000032:analysis*
D005233:Fatty Alcohols; D000069463:Olive Oil; D010938:Plant Oils; D013261:Sterols; D014315:Triterpenes; C077953:erythrodiol; D009828:Oleanolic Acid; C013330:uvaol