title: Determination of thevetin B in serum by fluorescence polarization immunoassay.
abstract: Thevetin B, a cardiac glycoside of Thevetia neriifolia Juss. seeds, was determined in serum by fluorescence polarization immunoassay. Anti-digitoxin antibody was used, thevetin B genin being structurally identical to digitoxigenin. Cross-reactivity of 94% was found by this method, for concentrations from 5 to 80 ng ml-1.
authors.affiliation: Laboratoire de Pharmacognosie, Faculté des Sciences, Chatenay Malabry, France.
authors.forename: E
authors.initials: E
authors.lastname: Uber-Bucek
authors.forename: M
authors.initials: M
authors.lastname: Hamon
authors.forename: C
authors.initials: C
authors.lastname: Pham Huy
authors.forename: H
authors.initials: H
authors.lastname: Dadoun
mesh_terms: D002298:Cardenolides / Q000097:blood*; D002301:Cardiac Glycosides / Q000097:blood*; D003429:Cross Reactions; D004074:Digitoxin / Q000097:blood; D016231:Fluorescence Polarization Immunoassay / Q000379:methods; D006801:Humans
chemical_list: D002298:Cardenolides; D002301:Cardiac Glycosides; C077740:thevetin B; D004074:Digitoxin