title: Multivariate statistical analysis of electron micrographs of a mammalian transcription initiation complex.
abstract: We describe the appearance of the murine ribosomal protein (rp) L32 gene transcription initiation complex as determined by electron image analysis. Using a fractionated nuclear extract enriched for transcription factors necessary for rpL32 gene transcription in vitro and a DNA fragment containing the rpL32 gene promoter, the transcription initiation complex was prepared and viewed by standard transmission electron microscopy. Image analysis demonstrated that the complex was a multilobed structure.
authors.affiliation: Department of Molecular Biology and Genetics, University of Guelph, Ontario, Canada.
authors.forename: G
authors.initials: G
authors.lastname: Harauz
authors.forename: E A
authors.initials: EA
authors.lastname: Welsh
mesh_terms: D002478:Cells, Cultured; D007091:Image Processing, Computer-Assisted; D046911:Macromolecular Substances; D008854:Microscopy, Electron; D012269:Ribosomal Proteins / Q000235:genetics* / Q000648:ultrastructure*; D014158:Transcription, Genetic
chemical_list: D046911:Macromolecular Substances; D012269:Ribosomal Proteins