title: Integration of image analysis and robotics into a fully automated colony picking and plate handling system.
abstract: We describe here the integration of image analysis and robotics to produce a fully automated colony picking/plate handling system. Biological tests were performed to verify its performance in terms of sterilisation and accuracy of picking. The machine was then used by a single operative to pick a 36,000 clone cDNA library in approximately 42 hrs over 5 days.
authors.affiliation: MRC Laboratory of Molecular Biology, MRC Centre, Cambridge, UK.
authors.forename: P
authors.initials: P
authors.lastname: Jones
authors.forename: A
authors.initials: A
authors.lastname: Watson
authors.forename: M
authors.initials: M
authors.lastname: Davies
authors.forename: S
authors.initials: S
authors.lastname: Stubbings
mesh_terms: D001331:Automation / Q000295:instrumentation*; D010582:Bacteriophage lambda / Q000235:genetics; D003001:Cloning, Molecular; D015169:Colony Count, Microbial / Q000295:instrumentation*; D004926:Escherichia coli / Q000235:genetics; D015723:Gene Library; D007091:Image Processing, Computer-Assisted / Q000295:instrumentation; D016133:Polymerase Chain Reaction; D012371:Robotics / Q000295:instrumentation