High-performance capillary electrophoresis of unsaturated oligosaccharides derived from glycosaminoglycans by digestion with chondroitinase ABC as 1-phenyl-3-methyl-5-pyrazolone derivatives.
This paper proposes a new method for simultaneous analysis of unsaturated disaccharides derived from glycosaminoglycans by enzymatic digestion with chondroitinase ABC, based on high-performance capillary electrophoresis (HPCE) of their 1-phenyl-3-methyl-5-pyrazolone derivatives. The O-sulphate group is stable in this derivatization, and this method allows reproducible microdetermination of glycosaminoglycans. This paper also demonstrates the applicability of this method to estimation of urinary chondroitin sulphates. Urinary creatinine as an inherent internal standard could also be estimated by HPCE, though in another mode of separation, i.e. ion-exchange electrokinetic chromatography.
Honda
S
S
Faculty of Pharmaceutical Sciences, Kinki University, Higashi-Osaka, Japan.
Ueno
T
T
Kakehi
K
K
D000983:Antipyrine / Q000031:analogs & derivatives* / Q000737:chemistry; D002808:Chondroitin Lyases; D002852:Chromatography, Ion Exchange; D003404:Creatinine / Q000032:analysis / Q000652:urine; D000077553:Edaravone; D004586:Electrophoresis; D006025:Glycosaminoglycans / Q000032:analysis*; D006801:Humans; D006868:Hydrolysis; D009844:Oligosaccharides / Q000032:analysis*
D006025:Glycosaminoglycans; D009844:Oligosaccharides; D003404:Creatinine; D002808:Chondroitin Lyases; D000077553:Edaravone; D000983:Antipyrine