Synthesis, cytotoxicity, hypolipidemic and anti-inflammatory activities of amine-boranes and esters of boron analogues of choline and thiocholine.
Boron analogues of carbamoylcholine and thiocholine and esters of these analogues were prepared. These compounds were fairly stable toward hydrolysis and demonstrated moderate anti-inflammatory and hypolipidemic activities in mice. The hypolipidemic activity of the compounds at a dose of 8 mg/kg/day was equivalent in reducing lipid levels in serum to those of clofibrate at 150 mg/kg/day and lovastatin at 8 mg/kg/day. The compounds demonstrated significant cytotoxic activity against the growth of murine and human tumor cells; all were active against the growth of human HeLa-S3 uterine suspended cells, and some were active against murine L1210 lymphoid leukemia, human Tmolt3 leukemia cells, colorectal adenocarcinoma, KB nasopharynx, osteosarcoma, and glioma. These studies demonstrated that antimetabolite analogues of acetylcholine exhibit the same types of pharmacological activity as other boron-substituted betaine and amino acids. Furthermore, a strong positive correlation exists between hypolipidemic activity and cytotoxicity for these new choline derivatives, as has previously been demonstrated for other boron-containing amino acids, amides, esters, and peptides.
Sood
A
A
Gross Chemistry Laboratory, Duke University, Durham, NC 27706.
Sood
C K
CK
Spielvogel
B F
BF
Hall
I H
IH
Wong
O T
OT
D000588:Amines / Q000138:chemical synthesis / Q000494:pharmacology* / Q000633:toxicity; D000818:Animals; D000893:Anti-Inflammatory Agents; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000138:chemical synthesis / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000138:chemical synthesis / Q000494:pharmacology* / Q000633:toxicity; D001880:Boranes / Q000138:chemical synthesis / Q000494:pharmacology* / Q000633:toxicity; D001896:Boron Compounds / Q000138:chemical synthesis / Q000494:pharmacology* / Q000633:toxicity; D002219:Carbamates / Q000138:chemical synthesis / Q000494:pharmacology / Q000633:toxicity; D002794:Choline / Q000031:analogs & derivatives*; D004355:Drug Stability; D004952:Esters / Q000138:chemical synthesis / Q000494:pharmacology* / Q000633:toxicity; D006801:Humans; D008055:Lipids / Q000097:blood; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009374:Neoplasms, Experimental / Q000188:drug therapy; D013860:Thiocholine / Q000031:analogs & derivatives*
D000588:Amines; D000893:Anti-Inflammatory Agents; D000894:Anti-Inflammatory Agents, Non-Steroidal; D000970:Antineoplastic Agents; D001880:Boranes; D001896:Boron Compounds; D002219:Carbamates; D004952:Esters; D008055:Lipids; D013860:Thiocholine; D002794:Choline