title: Crystallization and preliminary X-ray study of the cathepsin B complexed with CA074, a selective inhibitor.
abstract: Cathepsin B from bovine spleen has been purified and crystallized as a complex with a specific inhibitor CA074 [N-(L-3-trans-propylcarbamoyloxirane-2-carbonyl)-L- isoleucyl-L-proline], using the hanging-drop method. The complex crystals obtained from 50 mM-citrate buffer (pH 3.5) belong to the tetragonal space group P4(1) (or P4(3)) with a = 73.06 A and c = 141.59 A, and diffract beyond 2.2 A resolution. There are two complex molecules per asymmetric unit giving a packing density of 3.37 A3/Da and indicating a high solvent content of 63.5%.
authors.affiliation: Department of Physical Chemistry, Osaka University of Pharmaceutical Sciences, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Yamamoto
authors.forename: T
authors.initials: T
authors.lastname: Kaji
authors.forename: K
authors.initials: K
authors.lastname: Tomoo
authors.forename: T
authors.initials: T
authors.lastname: Ishida
authors.forename: M
authors.initials: M
authors.lastname: Inoue
authors.forename: M
authors.initials: M
authors.lastname: Murata
authors.forename: K
authors.initials: K
authors.lastname: Kitamura
mesh_terms: D000818:Animals; D002401:Cathepsin B / Q000737:chemistry* / Q000378:metabolism; D002417:Cattle; D003460:Crystallization; D004151:Dipeptides / Q000737:chemistry* / Q000378:metabolism; D046911:Macromolecular Substances; D008956:Models, Chemical; D014961:X-Ray Diffraction
chemical_list: D004151:Dipeptides; D046911:Macromolecular Substances; C068250:N-(3-propylcarbamoyloxirane-2-carbonyl)-isoleucyl-proline; D002401:Cathepsin B