title: Gilvocarcin V exhibits both equilibrium DNA binding and UV light induced DNA adduct formation which is sequence context dependent.
abstract: The relative degree of both equilibrium binding and of ultraviolet light induced adduct formation for the antitumor antibiotic gilvocarin V with two hexaecamer DNA sequence isomers, d[ATATATAGCTATATAT]2 and d[AAAAAAAGCTTTTTTT]2, was assessed. The experiments reveal that gilvocarin V binds, under equilibrium conditions, and reacts, in the presence of exogenously applied UV light, more efficiently with the alternating purine:pyrimidine sequence hexadecamer than the homopurine:homopyrimidine duplex at identical gilvocarcin V to DNA duplex ratios. DNAse I digests of adduct containing duplexes derived from the d[AAAAAAAGCTTTTTTT]2 duplex, identified and isolated using gel shift assays employing denaturing polyacrylamide gels, confirm that gilvocarcin V adducts can be formed with thymine residues but suggest that adduct formation with either adenine or guanine residues is also possible.
authors.affiliation: Second Department of Dermatology, University of Vienna, Austria.
authors.forename: R M
authors.initials: RM
authors.lastname: Knobler
authors.forename: F B
authors.initials: FB
authors.lastname: Radlwimmer
authors.forename: M J
authors.initials: MJ
authors.lastname: Lane
mesh_terms: D000617:Aminoglycosides; D000900:Anti-Bacterial Agents / Q000378:metabolism* / Q000528:radiation effects; D000903:Antibiotics, Antineoplastic / Q000378:metabolism* / Q000528:radiation effects; D001483:Base Sequence; D003374:Coumarins; D003850:Deoxyribonuclease I / Q000378:metabolism; D006027:Glycosides; D008969:Molecular Sequence Data; D016296:Mutagenesis / Q000235:genetics / Q000528:radiation effects; D009838:Oligodeoxyribonucleotides / Q000378:metabolism* / Q000528:radiation effects; D014466:Ultraviolet Rays
chemical_list: D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D000903:Antibiotics, Antineoplastic; D003374:Coumarins; D006027:Glycosides; D009838:Oligodeoxyribonucleotides; C031781:gilvocarcin V; D003850:Deoxyribonuclease I