[The effect of physicochemical factors on the activity of DNA-dependent DNA polymerases in Acholeplasma laidlawii PG-8].
The biological and physico-chemical properties of DNA-dependent DNA-polymerases of Acholeplasma laidlawii PG-8 have been studied. The optimal parameters of maximal enzymatic activity are determined. It is stated that N-ethylmaleimide in concentration of 1 mM activated DNA-polymerase I by 52%, whereas DNA-polymerase II with reagent concentration of 0.5 mM demonstrated the peak of activity exceeding the control only by 10%. Spermidine in concentration of 1.5 mM for the first form of DNA-polymerase and 0.15 mM-for the second one increased the ability of both forms of polymerases to synthesize DNA by 10%. Aphidicolin added to the reaction medium up to concentration of 10 mg/ml decreased activity of forms I and II of enzymes by 83 and 68%, respectively. The presence of 0.6 mM of EDTA in the medium also negatively affected the activity of polymerases inhibiting it by 83% in form I and by 77%-in form II.
Bezuglyĭ
S V
SV
Skripal'
I G
IG
Babichev
V V
VV
Malinovskaia
L P
LP
D000128:Acholeplasma laidlawii / Q000187:drug effects / Q000201:enzymology*; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004259:DNA-Directed DNA Polymerase / Q000737:chemistry* / Q000187:drug effects / Q000302:isolation & purification; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D013696:Temperature
D004259:DNA-Directed DNA Polymerase
Vliianie nekotorykh fiziko-khimicheskikh faktorov na aktivnost' DNK-zavisimykh DNK-polimeraz Acholeplasma laidlawii PG-8.