Ca(2+)-independent contraction of uterine smooth muscle induced by vanadate and its inhibition by Ca2+.
Vanadate, 30 microM, contracts uterine smooth muscle of estrogen-dominated non-pregnant rats in Ca(2+)-free medium after preincubation with 3 mM EGTA. In spite of the phosphorylation of the myosin light chain during this contraction, studies with fura-2 suggested that this contraction was not accompanied by an increase in the cytosolic Ca2+ level. Inhibitors of the myosin light chain kinase and protein kinase C partly inhibited this contraction. Vanadate seems to enter the cell through anion channels to inhibit phosphatases, resulting in phosphorylation via basal activities of the myosin light chain kinase and protein kinase C. An increase in the cytosolic free Ca2+ level resulted in relaxation of the contracting muscle in the same manner as in the oxytocin-induced Ca(2+)-free contraction.
Fukuzaki
A
A
Department of Molecular Pharmacology, Meiji College of Pharmacy, Tokyo, Japan.
Suga
O
O
Karibe
H
H
Miyauchi
Y
Y
Gokita
T
T
Uchida
M K
MK
D000470:Alkaloids / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000494:pharmacology*; D002227:Carbazoles; D005260:Female; D016257:Fura-2 / Q000032:analysis; D066298:In Vitro Techniques; D007211:Indoles; D009119:Muscle Contraction / Q000187:drug effects*; D009130:Muscle, Smooth / Q000187:drug effects*; D009219:Myosin-Light-Chain Kinase / Q000037:antagonists & inhibitors; D010121:Oxytocin / Q000494:pharmacology; D051381:Rats; D014599:Uterus / Q000187:drug effects; D014638:Vanadates / Q000037:antagonists & inhibitors / Q000494:pharmacology*
D000470:Alkaloids; D002227:Carbazoles; D007211:Indoles; C063769:KT 5926; D014638:Vanadates; D010121:Oxytocin; D009219:Myosin-Light-Chain Kinase; D002118:Calcium; D016257:Fura-2