Import and processing of the precursor of the delta subunit of tobacco chloroplast ATP synthase.
A cDNA clone encoding the complete precursor of the delta subunit of chloroplast ATP synthase has been isolated from a tobacco (Nicotiana tabacum) leaf cDNA library in lambda gt11. The 880 bp insert encodes a polypeptide of 248 amino acid residues, of which 61 residues constitute an N-terminal presequence and 187 residues make up the mature delta subunit. Transcription and translation of the cDNA in vitro produced a protein of 29 kDa which was imported by isolated pea chloroplasts and processed to the mature 20 kDa subunit. The delta subunit precursor was processed to the mature size by a processing peptidase present in pea stromal extracts. Hybridisation of the cDNA to Southern blots of tobacco genomic DNA suggests the presence of two genes in the haploid genome.
Napier
J A
JA
Department of Plant Sciences, University of Cambridge, UK.
Larsson
K H
KH
Madueño
F
F
Gray
J C
JC
D000595:Amino Acid Sequence; D001483:Base Sequence; D001692:Biological Transport; D002736:Chloroplasts / Q000378:metabolism*; D003001:Cloning, Molecular; D010450:Endopeptidases / Q000378:metabolism; D015723:Gene Library; D008565:Membrane Proteins; D008969:Molecular Sequence Data; D010947:Plants, Toxic; D011499:Protein Processing, Post-Translational; D021382:Protein Sorting Signals / Q000235:genetics; D006180:Proton-Translocating ATPases / Q000096:biosynthesis* / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D012697:Serine Endopeptidases; D014026:Nicotiana / Q000378:metabolism*
D008565:Membrane Proteins; D021382:Protein Sorting Signals; D010450:Endopeptidases; D012697:Serine Endopeptidases; C026515:type I signal peptidase; D006180:Proton-Translocating ATPases