title: High reactivity of aortic fibroblasts to vasoactive agents: endothelins, bradykinin and nucleotides.
abstract: Cultured aortic fibroblasts express high affinity Et-1 binding sites that poorly discriminate between Et-1 and Et-3. Both endothelins activate phospholipase C hence indicating the presence of ETB receptors. Fibroblasts respond to bradykinin by large activations of phospholipase C and increases in [Ca2+]i in a manner that was abolished by D-Arg, [Hyp3,Thi5,8,D-Phe7]-bradykinin, thus indicating the presence of B2 kinin receptors. Finally, ATP, UTP and ADP increases [Ca2+]i in aortic fibroblasts via a nucleotide receptor that has a higher affinity for ATP and UTP (3 microM) than for ADP (50 microM) and that is distinct from P2x and P2y purinoceptors.
authors.affiliation: Institut de Pharmacologie Moléculaire et Cellulaire du CNRS, Valbonne, France.
authors.forename: R
authors.initials: R
authors.lastname: Marsault
authors.forename: P
authors.initials: P
authors.lastname: Vigne
authors.forename: C
authors.initials: C
authors.lastname: Frelin
mesh_terms: D000244:Adenosine Diphosphate / Q000494:pharmacology*; D000255:Adenosine Triphosphate / Q000494:pharmacology*; D000818:Animals; D001013:Aorta, Thoracic / Q000378:metabolism*; D001920:Bradykinin / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D016232:Endothelins / Q000378:metabolism / Q000494:pharmacology*; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D007700:Kinetics; D009131:Muscle, Smooth, Vascular / Q000378:metabolism*; D051381:Rats; D011921:Rats, Inbred WKY; D017466:Receptors, Endothelin / Q000378:metabolism; D010738:Type C Phospholipases / Q000378:metabolism*; D014544:Uridine Triphosphate / Q000494:pharmacology*
chemical_list: D016232:Endothelins; D017466:Receptors, Endothelin; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D010738:Type C Phospholipases; D001920:Bradykinin; D002118:Calcium; D014544:Uridine Triphosphate