title: Anchored anti-HLA class I monoclonal antibody fails to induce inhibition of PHA-activated lymphocytes proliferation.
abstract: It is known that anti-HLA Class I antibodies inhibit the proliferative response of PHA-activated T-lymphocytes. We found that plastic- or sepharose-linked anti-HLA Class I monoclonal antibody 01.65 does not inhibit either [3H]Thymidine incorporation or recruitment in the cell cycle, nor does it reduce the expression of c-myc mRNA and the membrane expression of Interleukin-2 Receptor and Transferrin Receptor. Furthermore, particulate Protein Kinase C is not affected by anchored anti-HLA Class I monoclonal antibody 01.65. We suggest that anti-HLA Class I monoclonal antibody may act through crosslinking or internalization of HLA Class I antigens.
authors.affiliation: Institute of Medical Genetics, University of Ferrara, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Rubini
authors.forename: R
authors.initials: R
authors.lastname: Selvatici
authors.forename: P
authors.initials: P
authors.lastname: Orlando
authors.forename: A
authors.initials: A
authors.lastname: Balboni
authors.forename: S
authors.initials: S
authors.lastname: Boninsegna
authors.forename: E
authors.initials: E
authors.lastname: Gandini
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology / Q000494:pharmacology*; D002453:Cell Cycle; D002478:Cells, Cultured; D015395:Histocompatibility Antigens Class I / Q000276:immunology*; D006801:Humans; D007700:Kinetics; D008213:Lymphocyte Activation / Q000187:drug effects / Q000276:immunology*; D008214:Lymphocytes / Q000276:immunology* / Q000378:metabolism; D010835:Phytohemagglutinins; D011493:Protein Kinase C / Q000378:metabolism; D015375:Receptors, Interleukin-2 / Q000096:biosynthesis; D011990:Receptors, Transferrin / Q000096:biosynthesis; D013936:Thymidine / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D015395:Histocompatibility Antigens Class I; D010835:Phytohemagglutinins; D015375:Receptors, Interleukin-2; D011990:Receptors, Transferrin; C028784:phytohemagglutinin-P; D011493:Protein Kinase C; D013936:Thymidine