7-Methylguanine levels in DNA of smokers' and non-smokers' total white blood cells, granulocytes and lymphocytes.
The effect of smoking was investigated on the formation of 7-methylguanines in human peripheral white blood cells. DNA was isolated from total white blood cells, granulocytes and lymphocytes from 10 smokers and 10 non-smokers. 32P-Postlabeling was performed by using anion-exchange chromatography enrichment of adducts. In smokers the mean DNA adduct levels were 6.9, 4.7 and 23.6 7-methylguanine residues/10(7) nucleotides in total white blood cells, granulocytes and lymphocytes respectively. The corresponding values in non-smokers were 3.4, 2.8 and 13.5 adducts/10(7) nucleotides. The mean adduct level was significantly higher in lymphocytes than in total white blood cells or granulocytes both in smokers and in non-smokers. The mean adduct levels differed significantly between smokers and non-smokers.
Mustonen
R
R
Institute of Occupational Health, Helsinki, Finland.
Hemminki
K
K
D000328:Adult; D001345:Autoradiography; D002852:Chromatography, Ion Exchange; D004249:DNA Damage; D005260:Female; D006098:Granulocytes / Q000378:metabolism*; D006147:Guanine / Q000031:analogs & derivatives* / Q000097:blood; D006801:Humans; D007962:Leukocytes / Q000378:metabolism*; D008214:Lymphocytes / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D012907:Smoking / Q000097:blood*
D006147:Guanine; C008450:7-methylguanine