title: Detection of early xerophthalmia by impression cytology and rose Bengal staining--a comparative study.
abstract: Twenty two cases were studied for early detection of Xerophthalmia by impression cytology and Rose Bengal staining. Patients were below 6 years of age and admitted in the paediatric ward of Dhaka Medical College Hospital. Assessment were done by conjunctival impression cytology and staining with 1% Rose Bengal dye. Estimation of serum retinol level was done by HPLC to correlate conjunctival findings with biochemical status. The mean age of the subjects were 3.15 +/- 1.75. Rose Bengal staining was positive in 40% cases and impression cytology was in 60% cases. The mean serum retinol level of the diseased children were 10.5 +/- 3. ug/dl. The sensitivity of Rose Bengal staining was 53.3% and that of impression cytology was 80% in detecting vitamin A deficiency when compared with serum retinol level. The specificity of Rose Bengal staining was 40% and that of impression cytology was 100%. Sensitivity of impression cytology was 100% in patient with retinol level 10 ug/dl.
authors.affiliation: Dept of Ophthalmology, Dhaka Medical College Hospital.
authors.forename: A N
authors.initials: AN
authors.lastname: Khan
authors.forename: S
authors.initials: S
authors.lastname: Huda
authors.forename: A N
authors.initials: AN
authors.lastname: Ahmed
authors.forename: T
authors.initials: T
authors.lastname: Hossain
authors.forename: N
authors.initials: N
authors.lastname: Sultana
authors.forename: S M
authors.initials: SM
authors.lastname: Ali
mesh_terms: D002675:Child, Preschool; D003228:Conjunctiva / Q000473:pathology*; D003581:Cytodiagnosis; D006801:Humans; D012395:Rose Bengal; D014801:Vitamin A / Q000097:blood; D014802:Vitamin A Deficiency / Q000097:blood / Q000150:complications / Q000175:diagnosis; D014985:Xerophthalmia / Q000175:diagnosis* / Q000209:etiology
chemical_list: D014801:Vitamin A; D012395:Rose Bengal