title: Simultaneous administration of lactulose and 51Cr-ethylenediaminetetraacetic acid. A test to distinguish colonic from small-intestinal permeability change.
abstract: In normal adults intestinal permeation of ingested 51Cr-ethylenediaminetetraacetic acid (EDTA) is greater than that of lactulose. This difference is abolished in patients with ileostomies, suggesting that it results from colonic permeation of 51Cr-EDTA, which, unlike lactulose, resists bacterial degradation. To investigate the effect of an increase in colonic permeability on absorption of the two molecules, lactulose (5 g) and 51Cr-EDTA (50 microCi) were given orally in isosmolar solution to 11 patients with colitis, and their 24-h urinary excretion measured. By comparison the effect of an increase in small-intestinal permeability induced by ingestion of a hyperosmolar solution (4240 mosm/l) was measured in 10 healthy adults. Hyperosmolar stress increased the 24-h urinary excretion of 51Cr-EDTA above the normal mean + 2 standard deviations (3.31%) in all 10 healthy subjects, and in all of these excretion of lactulose was also increased (greater than 1.06%). In contrast, although seven colitics had a urinary excretion of 51Cr-EDTA above the normal mean + 2 SD, in only two of these patients was recovery of lactulose increased. This suggests that simultaneous administration of lactulose and 51Cr-EDTA may enable permeability changes affecting the colon alone to be distinguished from those involving the small intestine.
authors.affiliation: Gastrointestinal Laboratory, Rayne Institute, St Thomas' Hospital, London, U.K.
authors.forename: A P
authors.initials: AP
authors.lastname: Jenkins
authors.forename: W S
authors.initials: WS
authors.lastname: Nukajam
authors.forename: I S
authors.initials: IS
authors.lastname: Menzies
authors.forename: B
authors.initials: B
authors.lastname: Creamer
mesh_terms: D000328:Adult; D002860:Chromium Radioisotopes; D003093:Colitis, Ulcerative / Q000378:metabolism; D003106:Colon / Q000378:metabolism*; D003424:Crohn Disease / Q000378:metabolism; D004492:Edetic Acid / Q000008:administration & dosage* / Q000493:pharmacokinetics; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism; D007421:Intestine, Small / Q000378:metabolism*; D007792:Lactulose / Q000008:administration & dosage* / Q000493:pharmacokinetics; D008875:Middle Aged; D009994:Osmolar Concentration; D010539:Permeability
chemical_list: D002860:Chromium Radioisotopes; D007792:Lactulose; D004492:Edetic Acid