Non-specific natural cytotoxic factor released from bovine peripheral blood lymphocytes.
Natural cytotoxicity against bovine leukemia cells (PC-3 cells) was found in bovine peripheral blood lymphocytes (PBL), and in non-adherent cells but not in adherent cells to nylon-wool column. Natural cytotoxic cells (NCC), which have natural cytotoxic activity, are found in T cell-rich fraction. When NCC were cocultured with PC-3 cells, natural cytotoxic factor (NCF) was released rapidly from NCC, and dose-response curve for NCF was almost linear induction. Cytotoxicity against PC-3 cells by NCC or NCF was increased with an increment of incubation period. Cytotoxicity against K562 cells, CL-1 cells, M1 cells or EL-4 cells by NCF was almost the same level as that against PC-3 cells, but that against those cell lines by NCC was not found. NCF activity in culture fluid from NCC cocultured with K562 cells or CL-1 cells was lower than that from NCC cocultured with PC-3 cells.
Kosaka
T
T
Department of Veterinary Radiology, School of Veterinary Medicine, Nihon University, Kanagawa, Japan.
Kuwabara
M
M
Koide
F
F
D000818:Animals; D002417:Cattle / Q000276:immunology*; D003602:Cytotoxicity, Immunologic / Q000502:physiology*; D005260:Female; D006801:Humans; D055646:Killer Factors, Yeast; D008214:Lymphocytes / Q000276:immunology*; D008297:Male; D011506:Proteins / Q000276:immunology / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D055646:Killer Factors, Yeast; D011506:Proteins