title: A kinetic study of the role of band 3 anion transport protein in the transport of salicylic acid and other hydroxybenzoic acids across the human erythrocyte membrane.
abstract: The mechanism of transport of salicylic acid and five other hydroxybenzoic acids across the human erythrocyte membrane was investigated. The specific anion transport inhibitor, 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid, reduced but did not totally abolish transport of these acids. This observation suggests that these acids are transported by two parallel processes, one involving the band 3 anion transport protein channel and the other probably involving passive diffusion of the un-ionized molecule. The activation energies for membrane transport were large (99-127 kJ.mol-1), an observation that is consistent with the rate-limiting step for anion transport being the conformational change of the band 3 anion transport protein.
authors.affiliation: Department of Pharmacy, University of Sydney, Australia.
authors.forename: T
authors.initials: T
authors.lastname: Minami
authors.forename: D J
authors.initials: DJ
authors.lastname: Cutler
mesh_terms: D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D001457:Anion Exchange Protein 1, Erythrocyte / Q000037:antagonists & inhibitors / Q000502:physiology*; D027321:Anion Transport Proteins; D001692:Biological Transport; D002352:Carrier Proteins / Q000037:antagonists & inhibitors / Q000502:physiology*; D004910:Erythrocyte Membrane / Q000378:metabolism*; D006801:Humans; D062385:Hydroxybenzoates / Q000493:pharmacokinetics*; D007700:Kinetics; D012459:Salicylates / Q000493:pharmacokinetics*; D020156:Salicylic Acid; D013329:Structure-Activity Relationship; D013696:Temperature
chemical_list: D001457:Anion Exchange Protein 1, Erythrocyte; D027321:Anion Transport Proteins; D002352:Carrier Proteins; D062385:Hydroxybenzoates; D012459:Salicylates; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid; D020156:Salicylic Acid; D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid