Activation of phospholipases in platelets by polyclonal antibodies against a surface membrane protein.
In a previous paper we demonstrated using immunochemical techniques that propolypeptide of von Willebrand factor was present on the surface of resting platelets. In the present paper we show that polyclonal antibodies against propolypeptide of von Willebrand factor induce activation of phospholipase(s) in platelets and lead to platelet aggregation. The antibody-stimulation of platelets induced the synthesis of thromboxane A2 (TXA2). Furthermore, the aggregation was inhibited by aspirin and an antagonist of TXA2. Aspirin inhibited not only the aggregation but also the activation of arachidonic acid liberation from phospholipids, but the effect of aspirin on arachidonic acid liberation was cancelled by the combined effect of the antibodies and a TXA2 mimetic agonist, which itself did not activate arachidonic acid liberation. The antibody-induced activation of arachidonic acid liberation and the aggregation were blocked by cytochalasin B. All these results obtained with antibodies were quite similar to the results obtained with collagen.
Hashimoto
K
K
Department of Biological Sciences, Tokyo Institute of Technology, Japan.
Sekiya
F
F
Takagi
J
J
Tsukada
T
T
Sato
F
F
Saito
Y
Y
D000906:Antibodies / Q000502:physiology*; D001095:Arachidonic Acids / Q000378:metabolism; D001792:Blood Platelets / Q000201:enzymology*; D003571:Cytochalasin B / Q000494:pharmacology; D004789:Enzyme Activation; D006801:Humans; D007700:Kinetics; D010740:Phospholipases / Q000378:metabolism*; D010974:Platelet Aggregation; D011498:Protein Precursors / Q000276:immunology*; D013931:Thromboxanes / Q000096:biosynthesis; D014841:von Willebrand Factor / Q000276:immunology*
D000906:Antibodies; D001095:Arachidonic Acids; D011498:Protein Precursors; D013931:Thromboxanes; D014841:von Willebrand Factor; C067003:von Willebrand factor propolypeptide; D003571:Cytochalasin B; D010740:Phospholipases