Angiotensin II binding sites in frog kidney and adrenal.
Angiotensin II (AII) binding sites were localized and quantified in kidney and adrenal of the frog Rana temporaria by quantitative in vitro autoradiography. AII binding was present in kidney glomeruli and in interrenal tissue of the outer zone of the adrenal gland. Saturation experiments showed that [125I]-[Val5]AII binds to a single class of binding sites with a dissociation constant (Kd) of 548 +/- 125 pM in glomeruli and 593 +/- 185 pM in interrenal tissue (n = 8). The corresponding maximal binding capacities (Bmax) were 2.48 +/- 0.71 and 3.05 +/- 1.02 fmol/mm2, respectively. AII binding was displaced by unlabeled angiotensin analogues in the rank order: [Sar1]AII greater than human AII greater than [125I]-[Val5]AII = [Val5]AII = human AIII much greater than human AI. The AII binding sites in glomeruli and interrenal tissue suggest an influence of AII on glomerular filtration rate and adrenal steroid secretion to take part in osmomineral regulation of the frog.
Kloas
W
W
Department of Zoology II, University of Karlsruhe, Germany.
Hanke
W
W
D000311:Adrenal Glands / Q000737:chemistry*; D000804:Angiotensin II / Q000031:analogs & derivatives / Q000737:chemistry*; D000818:Animals; D001345:Autoradiography; D001667:Binding, Competitive; D006651:Histocytochemistry; D007668:Kidney / Q000737:chemistry*; D007700:Kinetics; D008297:Male; D011896:Rana temporaria; D011945:Receptors, Angiotensin / Q000737:chemistry* / Q000302:isolation & purification
D011945:Receptors, Angiotensin; D000804:Angiotensin II