Streptomyces lividans glycosylates an exoglucanase (Cex) from Cellulomonas fimi.
Exoglucanase Cex from Cellulomonas fimi is a glycoprotein [Langsford et al., J. Gen. Microbiol. 130 (1984) 1367-1376]. Cex produced by Streptomyces lividans from the cloned cex gene is also glycosylated. The extent and nature of glycosylation are similar for Cex from both organisms. The glycosylation affords protection against proteolysis for the enzymes from both organisms when they are bound to cellulose, but not in solution. The ability to glycosylate cloned gene products enhances the utility of Streptomyces as a host for the production of heterologous polypeptides.
MacLeod
A M
AM
Department of Microbiology, University of British Columbia, Vancouver, Canada.
Gilkes
N R
NR
Escote-Carlson
L
L
Warren
R A
RA
Kilburn
D G
DG
Miller
R C
RC
D000192:Actinomycetales / Q000201:enzymology*; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D043326:Glucan 1,3-beta-Glucosidase; D006031:Glycosylation; D006868:Hydrolysis; D010957:Plasmids; D013302:Streptomyces / Q000378:metabolism*; D001617:beta-Glucosidase / Q000378:metabolism*
D001617:beta-Glucosidase; D043326:Glucan 1,3-beta-Glucosidase