title: Multiple zinc finger forms resulting from developmentally regulated alternative splicing of a transcription factor gene.
abstract: Transcripts encoding the Drosophila putative transcription factor CF2 are subject to developmentally regulated alternative splicing, and they encode protein isoforms that differ in the number of zinc fingers. One testis-specific RNA encodes an isoform that includes three zinc fingers and a frame-shifted segment. Two other transcripts encode isoforms with six and seven zinc fingers which bind to distinct promoters and DNA target sequences. Thus, because of alternative splicing, a single gene appears to encode distinct DNA-binding proteins, each capable of regulating different gene sets in different tissues and developmental periods.
authors.affiliation: Department of Cellular and Developmental Biology, Harvard University, Cambridge, MA 02138.
authors.forename: T
authors.initials: T
authors.lastname: Hsu
authors.forename: J A
authors.initials: JA
authors.lastname: Gogos
authors.forename: S A
authors.initials: SA
authors.lastname: Kirsh
authors.forename: F C
authors.initials: FC
authors.lastname: Kafatos
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002823:Chorion; D004268:DNA-Binding Proteins / Q000235:genetics*; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000235:genetics; D005786:Gene Expression Regulation; D005796:Genes; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D016133:Polymerase Chain Reaction; D012326:RNA Splicing; D012333:RNA, Messenger / Q000235:genetics; D016415:Sequence Alignment; D014157:Transcription Factors / Q000235:genetics*; D016335:Zinc Fingers
chemical_list: D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D009838:Oligodeoxyribonucleotides; D012333:RNA, Messenger; D014157:Transcription Factors; C076904:Cf2 protein, Drosophila