title: Broad host range and promoter selection vectors for bacteria that interact with plants.
abstract: A plasmid vector, pGV910, and a derived cosmid, pRG930, have been constructed. Both contain the ColE1 and pVS1 origins of replication and are stably maintained in Escherichia coli, Agrobacterium tumefaciens, and Azorhizobium caulinodans ORS571. They are compatible with commonly used IncP cloning vectors, although pVS1 was classified as an IncP plasmid, unable to replicate in E. coli (Y. Itoh, J.M. Watson, D. Haas, and T. Leisinger, Plasmid 11:206-220, 1984). Promoter selection vectors were derived from both of these plasmids by using a promoterless beta-glucuronidase and/or beta-galactosidase gene. These vectors facilitate the study of gene expression in bacteria under particular environmental conditions. This is illustrated by the expression of the gusA gene under the control of a nod promoter in A. caulinodans nodulating stem-located infection sites on Sesbania rostrata.
authors.affiliation: Laboratorium voor Genetica, Universiteit Gent, Belgium.
authors.forename: G
authors.initials: G
authors.lastname: Van den Eede
authors.forename: R
authors.initials: R
authors.lastname: Deblaere
authors.forename: K
authors.initials: K
authors.lastname: Goethals
authors.forename: M
authors.initials: M
authors.lastname: Van Montagu
authors.forename: M
authors.initials: M
authors.lastname: Holsters
mesh_terms: D001419:Bacteria / Q000235:genetics*; D018407:Bacterial Physiological Phenomena; D003001:Cloning, Molecular; D015964:Gene Expression Regulation, Bacterial; D005822:Genetic Vectors; D005966:Glucuronidase / Q000235:genetics / Q000378:metabolism; D010944:Plants / Q000382:microbiology*; D010957:Plasmids; D011401:Promoter Regions, Genetic; D015183:Restriction Mapping
chemical_list: D005966:Glucuronidase