New method of detecting nitric oxide production.
Nitric oxide (NO) reacts with H2O2 to produce a rise in potent luminol-dependent chemiluminescence under neutral pH conditions. We applied this reaction to the continuous detection of NO released from the rat isolated perfused kidney with simultaneous pressure changes. Acetylcholine increased NO release dose-dependently with the pressure reduction. An NO synthesis inhibitor, NG-monomethyl-L-arginine, abolished the release. This inhibition was reversed by the addition of L-arginine.
Kikuchi
K
K
Faculty of Pharmaceutical Sciences, University of Tokyo, Japan.
Hayakawa
H
H
Nagano
T
T
Hirata
Y
Y
Sugimoto
T
T
Hirobe
M
M
D000818:Animals; D066298:In Vitro Techniques; D007668:Kidney / Q000378:metabolism; D008297:Male; D009569:Nitric Oxide / Q000032:analysis / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
D009569:Nitric Oxide