The study of the cultivation of Chinese hamster ovary and Bowes cell lines with poly(organophosphazene) membranes.
Sixteen poly(organophosphazenes) were prepared by reaction between polydichlorophosphazene (NPCl2)n and nucleophilic reagents such as phenoxides and amino compounds. Adhesive and colony formation percent were investigated using V-79 Chinese hamster cells on poly(organophosphazene) films. It was found that [NP(OC6H5)2-x(NHBu-n)x]n (x = 0.2, 1.8) gave the best percent adhesiveness. This value was similar to that of Falcon. On the other hand, [NP(OC6H5)-(NHBu-n)]n film showed best colony percent formation. This was of a higher value than that of Falcon. The [NP(OC6H5)2]n properties were poor for cultivation of useful Bowes and chinese hamster ovary cell lines in comparison with Cytodex III.
Kajiwara
M
M
Department of Applied Chemistry, Faculty of Engineering, Nagoya University, Japan.
D000818:Animals; D016466:CHO Cells / Q000166:cytology* / Q000187:drug effects; D002448:Cell Adhesion / Q000502:physiology; D002460:Cell Line; D003114:Colony-Forming Units Assay; D006224:Cricetinae; D003412:Cricetulus; D005260:Female; D008168:Lung / Q000166:cytology / Q000187:drug effects; D008567:Membranes, Artificial; D008863:Microspheres; D009943:Organophosphorus Compounds / Q000138:chemical synthesis / Q000737:chemistry*; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*
D008567:Membranes, Artificial; D009943:Organophosphorus Compounds; D011108:Polymers