A convenient and flexible approach for introducing linkers on bifunctional chelating agents.
The synthesis of a protected bifunctional analog of ethylenediaminetetraacetic acid (EDTA) is described. The molecule contains an aminobenzyl moiety that allows the easy attachment of the chelating agent to a wide variety of groups. Examples of reaction with the C-termini of two peptides are given. In the following paper, the two peptides are used to study the enzymatic cleavage of metal chelates from a monoclonal antibody.
Studer
M
M
Department of Chemistry, University of California, Davis 95616.
Meares
C F
CF
D000595:Amino Acid Sequence; D002614:Chelating Agents / Q000138:chemical synthesis*; D003432:Cross-Linking Reagents / Q000138:chemical synthesis*; D004492:Edetic Acid / Q000031:analogs & derivatives / Q000737:chemistry; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000737:chemistry; D010446:Peptide Fragments / Q000737:chemistry
C078240:4-nitrobenzyl-EDTA; D002614:Chelating Agents; D003432:Cross-Linking Reagents; D009842:Oligopeptides; D010446:Peptide Fragments; C057690:aminobenzyl-EDTA; C078239:alanyl-leucyl-alanyl-leucine; D004492:Edetic Acid