Isolation and long-term maintenance of differentiated adult chicken hepatocytes in primary culture.
Adult chicken hepatocytes were obtained by an adaptation of the two step in situ collagenase perfusion. Usually 0.5 to 1 x 10(9) cells were obtained, with 75 to 95% viability. Hepatocytes attached within 2 h when plated on plastic cell culture dishes and spread in 4 h, surviving for several months in a specific serum-free medium. These cells retained a typical parenchymal cell morphology and the ability to produce a specific protein (albumin) throughout the culture period. We hereby provide a suitable model for studying hepatic metabolism in birds.
Fraslin
J M
JM
E.N.S.A. Chaire de Génétique, Rennes, France.
Touquette
L
L
Douaire
M
M
Menezo
Y
Y
Guillemot
J C
JC
Mallard
J
J
D000418:Albumins / Q000096:biosynthesis; D000818:Animals; D002454:Cell Differentiation; D002455:Cell Division; D002460:Cell Line; D002469:Cell Separation / Q000379:methods*; D002470:Cell Survival; D002478:Cells, Cultured / Q000166:cytology* / Q000378:metabolism; D002645:Chickens; D003470:Culture Media; D008099:Liver / Q000166:cytology* / Q000378:metabolism
D000418:Albumins; D003470:Culture Media