[Effect of stress on translocation on the glucocorticoid receptor complex into the nuclei of rat hepatocytes].
The effect of 24-hour immobilization stress on the translocation of glucocorticoid-receptor complex into the nuclei of hepatocytes was studied in experiments on male Wistar rats weighing 150-180 g. The level of translocation of the steroid-receptor complexes was appraised by incubation (0 degree C, 1 hour) of hepatocyte nuclei (6 x 10(-6)) with a glucocorticoid-receptor complex which was obtained by preliminary saturation of diluted hepatic cytosol with triamcinolone 3H-acetonide (80 nM) for 3 hours with adsorption of unbound labeled steroid. Translocation of the glucocorticoid-receptor complex of hepatic cytosol of rats subjected to stress occurred in a significantly less number into the hepatocyte nuclei of intact rats than into those of rats which had suffered stress. In contrast, translocation of the glucocorticoid-receptor complex of hepatic cytosol of intact rats into the nuclei of hepatocytes of stressed rats was significantly higher than translocation into hepatocyte nuclei of intact rats. It is concluded that the level of inhibition and activation of translocation in the cytoplasm of hepatic cells increases in stress.
Golikov
P P
PP
D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D011965:Receptors, Glucocorticoid / Q000378:metabolism*; D013312:Stress, Physiological / Q000378:metabolism*
D011965:Receptors, Glucocorticoid
Vliianie stressa na translokatsiiu gliukokortikoidretseptor nogokompleksa v iadra gepatotsitov krys.