Determination of 4,4'-methylenedianiline in hydrolysed human urine by micro liquid chromatography with ultraviolet detection.
4,4'-Methylenedianiline was determined in human urine by micro liquid chromatography with ultraviolet detection. The combination of a thorough work-up and the high mass sensitivity of micro liquid chromatography gave the method very high sensitivity. Derivatization with pentafluoropropionic anhydride enhanced the resolution of the 4,4'-dimethylenedianiline peak. The detection limit, defined as blank plus three times the standard deviation of the blank, was 2 nmol/l of urine, for 10-microliters injection volumes. The detection limit, defined as three times the noise, was about ten times better. The within- and between-assay coefficients of variation were 4 and 6%, respectively, for samples containing 40 nmol/l. The method was applied for the monitoring of excreted 4,4'-methylenedianiline in urine, during epicutaneous skin hypersensitivity testing (patch testing).
Brunmark
P
P
Department of Occupational and Environmental Medicine, University Hospital, Lund Sweden.
Persson
P
P
Skarping
G
G
D000814:Aniline Compounds / Q000652:urine*; D002273:Carcinogens / Q000032:analysis*; D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D010328:Patch Tests; D014466:Ultraviolet Rays; D001559:Benzhydryl Compounds
D000814:Aniline Compounds; D002273:Carcinogens; C009505:4,4'-diaminodiphenylmethane; D001559:Benzhydryl Compounds