Comparative quantitative study of Ki-67 antibody staining in 78 B and T cell malignant lymphoma (ML) using two image analyser systems.
Total Ki-67 stained area percentage was studied in 32 B and 46 T malignant lymphomas (ML) using two different image analyser systems (TAS, Leitz; SAMBA TM 2005, TITN) respectively. The total Ki-67 area percentage was highly correlated to the number of Ki-67 positive cellular profiles (B-ML, r = 0.93; T-ML, r = 0.88), indicating that area percentage is a reliable alternative method to the manual cell counting. Image analysis allows quicker measurements, appropriate to large and strictly lymphomatous regions. The cell image processor (SAMBA TM 2005, TITN) linked to a color video camera was more suitable for immunohistochemical sections and allowed more automated and faster measurements than the texture analyser (TAS, Leitz) linked with a black and white camera. Alkaline phosphatase technique with fast red as chromogen was more suitable for the detection of Ki-67 stained area by thresholding than peroxidase technique with aminoethylcarbazol or with diaminobenzidine as chromogens. Significant differences were found between low and high grade in B and T ML according to the Kiel classification (mean values +/- SD of 7.7 +/- 3.8% and 16.6 +/- 6.2% in B-ML and of 10.2 +/- 7.9% and 25.6 +/- 16.3% in T-ML respectively). In follicular B-ML, considering follicular areas only, values were comparable to high grade ML; angioimmunoblastic-lymphadenopathy-like (AILD-type) T-ML belonging to low grade ML showed similar values to pleomorphic T-ML with medium and/or large cells belonging to high grade ML.(ABSTRACT TRUNCATED AT 250 WORDS)
Caulet
S
S
Department of Pathology Jacques Delarue, Hotel-Dieu, Paris, France.
Lesty
C
C
Raphael
M
M
Schoevaert
D
D
Brousset
P
P
Binet
J L
JL
Diebold
J
J
Delsol
G
G
D000469:Alkaline Phosphatase / Q000032:analysis; D000911:Antibodies, Monoclonal / Q000032:analysis* / Q000276:immunology; D002455:Cell Division; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000295:instrumentation / Q000379:methods*; D007150:Immunohistochemistry; D019394:Ki-67 Antigen; D016393:Lymphoma, B-Cell / Q000737:chemistry* / Q000201:enzymology / Q000473:pathology*; D016399:Lymphoma, T-Cell / Q000737:chemistry* / Q000201:enzymology / Q000473:pathology*; D009687:Nuclear Proteins / Q000276:immunology*
D000911:Antibodies, Monoclonal; D019394:Ki-67 Antigen; D009687:Nuclear Proteins; D000469:Alkaline Phosphatase