title: Cellular distribution of a colonic adenoma-associated antigen as defined by monoclonal antibody Adnab-9.
abstract: Adenomatous colonic polyps constitute a precursor for colorectal cancer. Antibodies to these precancerous lesions might identify specific early tumor antigens. Adnab-9 is a murine monoclonal antibody raised against membranes of colonic adenomas. Adnab-9 binding in colonic washings (effluent) correlates with the presence of colorectal cancer. Immunohistochemical staining with Adnab-9 shows cytoplasmic reactivity in scattered cells in 4 of 31 adenomatous tissue sections, 0 of 14 sections of colorectal cancer cells, and 1 of 8 normal-appearing colonic mucosa specimens examined. Adnab-9 recognized a dominant M(r) 87,000 protein species in tissue extracts in the membrane-bound fraction of effluent by Western blotting. Adnab-9 binding by enzyme-linked immunosorbent assay in adenomatous extracts is higher than cancer or normal tissue, is membrane-bound, and is absent from established colorectal cancer cell lines. This distribution and nature of immunostaining suggest that Adnab-9 recognizes a determinant associated with the membrane component of a subpopulation of adenoma cells which may have a role in early colorectal neoplasia.
authors.affiliation: Dept. of Medicine, Veterans Affairs Medical Center, Allen Park, Michigan.
authors.forename: M
authors.initials: M
authors.lastname: Tobi
authors.forename: B J
authors.initials: BJ
authors.lastname: Maliakkal
authors.forename: M A
authors.initials: MA
authors.lastname: Alousi
authors.forename: V
authors.initials: V
authors.lastname: Voruganti
authors.forename: M
authors.initials: M
authors.lastname: Shafiuddin
authors.forename: S
authors.initials: S
authors.lastname: Yang
authors.forename: M S
authors.initials: MS
authors.lastname: Gesell
authors.forename: T
authors.initials: T
authors.lastname: An
authors.forename: J S
authors.initials: JS
authors.lastname: Hatfield
authors.forename: S
authors.initials: S
authors.lastname: Fligiel
mesh_terms: D000236:Adenoma / Q000276:immunology*; D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm / Q000032:analysis*; D014408:Biomarkers, Tumor / Q000032:analysis; D015153:Blotting, Western; D003106:Colon / Q000276:immunology; D003110:Colonic Neoplasms / Q000276:immunology*; D015179:Colorectal Neoplasms / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007413:Intestinal Mucosa / Q000276:immunology; D014407:Tumor Cells, Cultured / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; C030139:colon-specific antigen