Echinococcus multilocularis: immunological study on the "Em2-positive" laminated layer during in vitro and in vivo post-oncospheral and larval development.
Echinococcus multilocularis oncospheres, primary vesicular cysts, and protoscolices were assessed in vitro and in vivo for their potential to synthesize a PAS-positive laminated layer containing monoclonal antibody (mAb) G11-binding Em2 antigen. The presence of Em2 antigen in developed oncospheres and cysts was subsequently correlated to the potential of in vivo development into a secondary metacestode in recipient host mice, which also responded by anti-Em2 serum antibody formation. In contrast, protoscolices failed to develop the "Em2-positive" layer in vitro under the selected experimental conditions. The failure to develop subsequently in vivo into a secondary metacestode was underlined by a lack of anti-Em2 serum antibody formation by the hosts. We furthermore developed a technique to obtain E. multilocularis clones by inoculating single oncospheres into recipient mice.
Gottstein
B
B
Institute of Parasitology, University of Zürich, Switzerland.
Deplazes
P
P
Aubert
M
M
D000818:Animals; D000909:Antibodies, Helminth / Q000096:biosynthesis*; D000947:Antigens, Helminth / Q000032:analysis* / Q000276:immunology; D004443:Echinococcosis / Q000469:parasitology*; D004446:Echinococcus / Q000254:growth & development / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D007814:Larva / Q000254:growth & development / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL
D000909:Antibodies, Helminth; D000947:Antigens, Helminth