The stereospecific interaction between chlorophylls and chlorophyllase. Possible implication for chlorophyll biosynthesis and degradation.
Chlorophyllase-catalyzed hydrolysis and esterification of chlorophylls, bacteriochlorophylls, and their free acids, respectively, depend on the configuration around the C-13(2) atom of the corresponding substrate. The data suggest that the enzyme interacts preferentially with compounds having the isocyclic carbomethoxy and the C-17 propionic residues facing opposite sides of the porphyrin macrocycle. The relevance of this observation to chlorophyll biosynthesis and degradation in vivo is briefly discussed.
Fiedor
L
L
Department of Biochemistry, Weizmann Institute of Science, Rehovot, Israel.
Rosenbach-Belkin
V
V
Scherz
A
A
D001429:Bacteriochlorophylls / Q000378:metabolism; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism*; D002734:Chlorophyll / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D010674:Pheophytins / Q000378:metabolism; D013237:Stereoisomerism; D013329:Structure-Activity Relationship
D001429:Bacteriochlorophylls; D010674:Pheophytins; D002734:Chlorophyll; D002265:Carboxylic Ester Hydrolases; C019875:chlorophyllase