[Studies on analysis of Belladonna alkaloids by capillary GC and GC-MSD].
A method for analyzing belladonna alkaloids--hyoscyamine, scopolamine, anisodamine and anisodine, by means of capillary GC and GC-MSD was described. The retention data and characteristic ions of the parent and TMS derivatives of these alkaloids are given and will be very useful for identification of unknown alkaloids. The derivatization of different silylation reagents was compared, and the derivatization with MSTFA was found to be better than with BSA and BSTFA. The method has been used for studying biotransformation of these alkaloids in biosynthesis. Analysis of anisodamine and anisodine by CGC and GC-MS has not been found in the literature. In addition, the advantages of TMS derivatization are also discussed.
Fang
H J
HJ
Institute of Materia Medica, Chinese Academy of Medical Sciences, Beijing.
Cheng
K D
KD
Xu
Y Q
YQ
D002849:Chromatography, Gas / Q000379:methods; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D012601:Scopolamine / Q000032:analysis; D012602:Scopolamine Derivatives / Q000032:analysis; D012991:Solanaceous Alkaloids / Q000032:analysis*
D012602:Scopolamine Derivatives; D012991:Solanaceous Alkaloids; C003922:anisodamine; D012601:Scopolamine; C012183:anisodine