Possible involvement of interleukin-1 in ischemic brain edema formation.
To determine the contribution of interleukin 1 (IL-1) on ischemic brain edema formation, the effect of recombinant human interleukin 1 beta (rhIL-1 beta), or zinc protoporphyrin (ZnPP) as an IL-1 blocker, on brain edema was studied in rats. The animals were subjected to 60 min of ischemia in a middle cerebral artery occlusion model. Immediately after reperfusion, rhIL-1 beta at a dose of 10 ng/2 microliters, or ZnPP at doses of 1 and 10 micrograms/2 microliters were topically applied into lateral cerebroventricle. In rhIL-1 beta-treated rats, ischemic brain edema formation was significantly increased in the dorsal and ventral areas of the caudate putamen 24 h after reperfusion, compared to that of vehicle-treated control rats. Furthermore, in ZnPP-treated rats, brain edema was decreased in both caudate-putamen areas. This suggests that IL-1 plays an important role in pathogenesis for post-ischemic brain edema.
Yamasaki
Y
Y
Department of Neurology, Tohoku University School of Medicine, Sendai, Japan.
Suzuki
T
T
Yamaya
H
H
Matsuura
N
N
Onodera
H
H
Kogure
K
K
D000818:Animals; D001834:Body Water / Q000378:metabolism; D001929:Brain Edema / Q000209:etiology*; D002545:Brain Ischemia / Q000150:complications*; D002421:Caudate Nucleus / Q000378:metabolism; D002540:Cerebral Cortex / Q000378:metabolism; D007375:Interleukin-1 / Q000037:antagonists & inhibitors / Q000494:pharmacology / Q000502:physiology*; D008297:Male; D011524:Protoporphyrins / Q000494:pharmacology; D011699:Putamen / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D011994:Recombinant Proteins
D007375:Interleukin-1; D011524:Protoporphyrins; D011994:Recombinant Proteins; C017803:zinc protoporphyrin