Changes in saliva of epileptic patients.
Unstimulated whole saliva samples of 27 indoor epileptic patients were studied on their protein composition using biochemical and immunochemical methods. A number of salivary proteins appeared at least partially to be hydrolyzed. In a number of saliva samples the concentration of carbohydrate-containing isoenzymes of amylase was reduced. In addition, the concentration of the 20 kD glycoprotein EP-GP was reduced by 60%. Sialic acid, the terminal sugar of the glycoproteins and mucins, was released for about 50% and in three salivas even nearly completely. Moreover, sialic acid- and fucose-containing epitopes could hardly be detected by monoclonal antibodies to human salivary mucins. As a consequence of this hydrolytic breakdown the saliva mediated aggregation of two S. sanguis strains had been reduced. In contrast, the aggregation of S. oralis had been maintained.
Nieuw Amerongen
A V
AV
Department of Oral Biochemistry, Vrije Universiteit, Amsterdam, The Netherlands.
Strooker
H
H
Oderkerk
C H
CH
Bank
R A
RA
Henskens
Y M
YM
Schenkels
L C
LC
Ligtenberg
A J
AJ
Veerman
E C
EC
D000293:Adolescent; D000328:Adult; D001422:Bacterial Adhesion; D002135:Calcium-Binding Proteins / Q000032:analysis; D004827:Epilepsy / Q000378:metabolism*; D005260:Female; D006023:Glycoproteins / Q000032:analysis*; D006801:Humans; D007527:Isoenzymes / Q000032:analysis; D008297:Male; D008875:Middle Aged; D008970:Molecular Weight; D009077:Mucins / Q000032:analysis; D010455:Peptides / Q000032:analysis; D010750:Phosphoproteins / Q000032:analysis; D055232:Proline-Rich Protein Domains; D012463:Saliva / Q000737:chemistry* / Q000201:enzymology; D012471:Salivary Proteins and Peptides / Q000032:analysis*; D012794:Sialic Acids / Q000032:analysis; D013291:Streptococcus / Q000502:physiology; D013298:Streptococcus sanguis / Q000502:physiology; D000516:alpha-Amylases / Q000032:analysis
D002135:Calcium-Binding Proteins; D006023:Glycoproteins; D007527:Isoenzymes; D009077:Mucins; D010455:Peptides; D010750:Phosphoproteins; D012471:Salivary Proteins and Peptides; D012794:Sialic Acids; D000516:alpha-Amylases