High-performance liquid chromatographic assay for amiodarone N-deethylation in microsomes of rat liver.
A reversed-phase high-performance liquid chromatographic assay using ultraviolet detection is described for determining the production of the major N-dealkylated metabolite of amiodarone in rat liver microsomes. The principal advantages of this method are its simple sample preparation (protein precipitation by acetonitrile), low detection limit for N-desethylamiodarone (0.05 mumol/l) and relatively short analysis time (16 min). Its analytical applicability is demonstrated by the comparison of the kinetic parameters (maximum velocity and Michaelis-Menten constant) between Sprague-Dawley and Dark-Agouti rats.
Trivier
J M
JM
Département de Biochimie, Hôpital Calmette, Lille, France.
Pommery
J
J
Libersa
C
C
Caron
J
J
Lhermitte
M
M
D000638:Amiodarone / Q000031:analogs & derivatives* / Q000032:analysis* / Q000378:metabolism; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005260:Female; D008862:Microsomes, Liver / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley
C036116:desethylamiodarone; D000638:Amiodarone