title: A new inhibitor of protein kinase C, RK-1409B (4'-demethylamino-4'-hydroxy-3'-epistaurosporine).
abstract: RK-1409B, a new inhibitor of protein kinase C, was isolated from the culture broth of Streptomyces platensis subsp. malvinus RK-1409. The structure was elucidated on the basis of spectroscopic analyses. RK-1409B inhibited protein kinase C in vitro and the morphological change of a human chronic leukemia cell line, K-562, induced by phorbol 12,13-dibutyrate with IC50 value of 0.4 microM.
authors.affiliation: Antibiotics Laboratory, RIKEN, Institute of Physical and Chemical Research, Saitama, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Koshino
authors.forename: H
authors.initials: H
authors.lastname: Osada
authors.forename: S
authors.initials: S
authors.lastname: Amano
authors.forename: R
authors.initials: R
authors.lastname: Onose
authors.forename: K
authors.initials: K
authors.lastname: Isono
mesh_terms: D002227:Carbazoles / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D006801:Humans; D007211:Indoles / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D007938:Leukemia / Q000188:drug therapy; D009682:Magnetic Resonance Spectroscopy; D011493:Protein Kinase C / Q000037:antagonists & inhibitors*; D013237:Stereoisomerism; D013302:Streptomyces / Q000737:chemistry*; D014407:Tumor Cells, Cultured / Q000187:drug effects
chemical_list: D002227:Carbazoles; D007211:Indoles; C077632:4'-demethylamino-4'-hydroxy-3'-epistaurosporine; D011493:Protein Kinase C