Molecular images of cereal proteins by STM.
Scanning tunnelling microscopy (STM) has been used to study a seed storage protein of wheat known as gamma-gliadin. The protein was deposited onto highly oriented pyrolytic graphite (HOPG) from solutions of trifluoroethanol (TFE) and 1% acetic acid. Samples were dried down and then scanned in air. Transmission electron microscopy (TEM) was also used to visualise the distribution of protein on the substrate. Small-angle X-ray scattering (SAXS) was used to compare the molecular size and shape obtained with those from the STM images.
Thomson
N H
NH
H.H. Wills Physics Laboratory, University of Bristol, UK.
Miles
M J
MJ
Tatham
A S
AS
Shewry
P R
PR
D005903:Gliadin / Q000648:ultrastructure*; D008854:Microscopy, Electron; D016252:Microscopy, Scanning Tunneling
D005903:Gliadin