Interleukin-6 and corticotrophin-releasing hormone mRNA are modulated during differentiation of human neuroblastoma cells.
Two cell clones [BE(2)-C and BE(2)-M17] derived from the human neuroblastoma cell line SK-N-BE(2) express corticotrophin-releasing hormone as well as interleukin-6 mRNA. Both genes are overexpressed, although with a different time course, following exposure to 5 microM retinoic acid, in parallel to the induction of neuroblastic differentiation. On the contrary, we are unable to detect interleukin-1 beta mRNA in these cell lines. Both cytokines are known to increase hypothalamic CRH mRNA. The production of cytokines and neuropeptides by neuroblastoma cells indicate a complex dialogue between tumour cells and anti-tumour immunity.
Stephanou
A
A
Department of Neuroendocrinology, Charing Cross and Westminster Medical School, Charing Cross Hospital, London, UK.
Melino
G
G
Knight
R A
RA
Annicchiarico-Petruzzelli
M
M
Sarlis
N J
NJ
Finazzi-Agro
A
A
Lightman
S L
SL
D015152:Blotting, Northern; D002454:Cell Differentiation; D003346:Corticotropin-Releasing Hormone / Q000235:genetics*; D015870:Gene Expression; D006801:Humans; D007375:Interleukin-1 / Q000235:genetics; D015850:Interleukin-6 / Q000235:genetics*; D007700:Kinetics; D009447:Neuroblastoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D012333:RNA, Messenger / Q000378:metabolism*; D014212:Tretinoin / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D007375:Interleukin-1; D015850:Interleukin-6; D012333:RNA, Messenger; D014212:Tretinoin; D003346:Corticotropin-Releasing Hormone