Identification of the number of genes encoding rat liver glutathione S-transferase Yc, and their close location on the genome.
We have tried to determine the number of genes for the rat liver glutathione S-transferase Yc, using specific probes prepared by polymerase chain reaction. DNA blot hybridization with specific probes and analysis in isolated genomic clones indicated that two copies of the Yc gene exist on the rat genome. Furthermore, DNA blot hybridization after clamped homogenous electric field gel electrophoresis indicated that the two copies of Yc gene family are closely located on the genome.
Yamada
T
T
Institute for Animal Experimentation, University of Tokushima School of Medicine, Japan.
Agui
T
T
Matsumoto
K
K
D000818:Animals; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004247:DNA / Q000737:chemistry / Q000235:genetics; D015342:DNA Probes; D004591:Electrophoresis, Polyacrylamide Gel; D005982:Glutathione Transferase / Q000235:genetics*; D008099:Liver / Q000201:enzymology*; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D051381:Rats; D015183:Restriction Mapping
D015342:DNA Probes; D004247:DNA; D005982:Glutathione Transferase