title: The genome of bovine ephemeral fever rhabdovirus contains two related glycoprotein genes.
abstract: A 3789 nucleotide region of the bovine ephemeral fever virus (BEFV) genome, located 1.65 kb downstream of the N gene, has been cloned and sequenced. The region contains two long open reading frames (ORFs) which are bounded by putative consensus (AACAGG) and polyadenylation (CATG[A]7) sequences and are separated by an intergenic region of 53 nucleotides. Discrete mRNAs corresponding to each ORF have been identified. The first ORF encodes a polypeptide comprising 623 residues which was identified by peptide sequencing as the virion G protein. The deduced amino acid sequence of the G protein includes putative signal and transmembrane domains and five potential glycosylation sites. The second ORF encodes a polypeptide of 586 amino acids which also has characteristics of a rhabdovirus glycoprotein, including putative signal and transmembrane domains and eight potential glycosylation sites, and appears to correspond to a 90-kDa nonstructural glycoprotein (GNS) identified in BEFV-infected cells (Walker et al. [1991] J. Gen. Virol. 72, 67-74). A database search indicated that both the G and GNS proteins share significant amino acid sequence homology with other rhabdovirus G proteins and with each other. Highest homology scores for each protein were with sigma virus and vesicular stomatitis virus serotypes.
authors.affiliation: CSIRO Division of Tropical Animal Production, Indooroopilly, Brisbane, Q, Australia.
authors.forename: P J
authors.initials: PJ
authors.lastname: Walker
authors.forename: K A
authors.initials: KA
authors.lastname: Byrne
authors.forename: G A
authors.initials: GA
authors.lastname: Riding
authors.forename: J A
authors.initials: JA
authors.lastname: Cowley
authors.forename: Y
authors.initials: Y
authors.lastname: Wang
authors.forename: S
authors.initials: S
authors.lastname: McWilliam
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004279:DNA, Viral; D016679:Genome, Viral; D006023:Glycoproteins / Q000235:genetics* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D012333:RNA, Messenger; D012209:Rhabdoviridae / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D017361:Viral Nonstructural Proteins; D014764:Viral Proteins / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D004279:DNA, Viral; D006023:Glycoproteins; D012333:RNA, Messenger; D017361:Viral Nonstructural Proteins; D014764:Viral Proteins; C077307:BEFV G protein, Bovine ephemeral fever virus; C077308:GNS protein, Bovine ephemeral fever virus