An enzymatic determination of hydrogen peroxide by using spectrophotometry.
A new spectrophotometric method for determining low hydrogen peroxide concentrations by using horseradish peroxidase in the presence of NADH at pH 7.5 has been described. Both total NADH consumption and initial reaction rate may be used for the determination. Using the NADH consumption, a linear response with respect to hydrogen peroxide was observed in the concentration range 7 x 10(-8)-2.5 x 10(-6) M. Due to the presence of superoxide dismutase, hydrogen peroxide is partly regenerated and an amplification of the signal results, which explains the sensitivity.
Alexandre
S
S
Laboratoire Polymères, Biopolymères, Membranes, U.A. n. 500, C.N.R.S., Université de Rouen, Mont-Saint-Aignan.
Telegdi
J
J
Boutelet
I
I
Julien
S
S
Vincent
J C
JC
D006735:Horseradish Peroxidase / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000032:analysis* / Q000493:pharmacokinetics; D009243:NAD / Q000378:metabolism; D012680:Sensitivity and Specificity; D013053:Spectrophotometry / Q000379:methods* / Q000706:statistics & numerical data
D009243:NAD; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase