A simple and rapid method for the detection and identification of mycobacteria using mycobactin.
A system was developed for the identification of mycobacteria such as Mycobacterium tuberculosis and M. avium, by thin layer chromatography of 55Fe-labelled mycobactin. Approximately 2 x 10(3) mycobacteria were detected within 24 h and little operator time or skill was required. M. avium, M. intracellulare and M. scrofulaceum were found to have lower requirements for iron than other mycobacteria and this may influence their growth in host organisms.
Barclay
R
R
Department of Microbiology, University of Reading.
Furst
V
V
Smith
I
I
D002855:Chromatography, Thin Layer / Q000379:methods; D002965:Classification / Q000379:methods*; D007501:Iron / Q000378:metabolism; D009161:Mycobacterium / Q000145:classification / Q000254:growth & development / Q000302:isolation & purification*; D009162:Mycobacterium avium / Q000145:classification / Q000254:growth & development / Q000302:isolation & purification; D015359:Mycobacterium scrofulaceum / Q000145:classification / Q000254:growth & development / Q000302:isolation & purification; D009169:Mycobacterium tuberculosis / Q000145:classification / Q000254:growth & development / Q000302:isolation & purification; D010080:Oxazoles / Q000737:chemistry / Q000302:isolation & purification*; D012680:Sensitivity and Specificity
D010080:Oxazoles; C018608:mycobactins; D007501:Iron