Differential regulation of putrescine uptake in Trypanosoma cruzi and other trypanosomatids.
Putrescine uptake in Trypanosoma cruzi epimastigotes is 10 to 50-fold higher than in Leishmania mexicana or Crithidia fasciculata. Polyamine transport in all these trypanosomatids is an energy-dependent process strongly inhibited by the presence of 2,4-dinitrophenol or KCN. Putrescine uptake in T. cruzi and L. mexicana was markedly decreased by the proton ionophore carbonylcyanide m-chlorophenylhydrazone but it was not affected by ouabain, a Na(+)-K+ pump inhibitor. The depletion of intracellular polyamines by treatment of parasite cultures with alpha-difluoromethylornithine elicited a marked induction of putrescine uptake in L. mexicana and C. fasciculata by increasing considerably the Vmax of this process. Conversely, the uptake of putrescine in T. cruzi was essentially unchanged by the same treatment. The differential regulation of putrescine transport in T. cruzi might be related to some distinctive features of polyamine metabolism in this parasite.
González
N S
NS
Instituto de Investigaciones Bioquímicas Fundación Campomar, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina.
Ceriani
C
C
Algranati
I D
ID
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D016831:Crithidia fasciculata / Q000187:drug effects / Q000378:metabolism*; D000518:Eflornithine / Q000494:pharmacology; D006706:Homeostasis; D007700:Kinetics; D007894:Leishmania mexicana / Q000187:drug effects / Q000378:metabolism*; D011700:Putrescine / Q000378:metabolism*; D013696:Temperature; D014349:Trypanosoma cruzi / Q000187:drug effects / Q000378:metabolism*
D011700:Putrescine; D000518:Eflornithine