title: Cytokine dysregulation in AIDS: in vivo overexpression of mRNA of tumor necrosis factor-alpha and its correlation with that of the inflammatory cytokine GRO.
abstract: The human immunodeficiency virus establishes an intimate interaction with the immune system. The virus can use cytokines, such as tumor necrosis factor-alpha (TNF-alpha) and interleukin-1 (Il-1), to regulate its own expression by modifying the normal immunoregulatory network. We demonstrate that mRNA of the cytokine TNF-alpha from peripheral blood mononuclear cells is overexpressed in virtually all patients with AIDS who do not have active opportunistic infections compared with uninfected volunteers (p < 0.0001). This overexpression correlates with elevated mRNA levels of the recently discovered GRO (p < 0.05), a cytokine involved in the inflammatory response.
authors.affiliation: Department of Medicine, Beth Israel Hospital, Boston, Massachusetts.
authors.forename: B J
authors.initials: BJ
authors.lastname: Dezube
authors.forename: A B
authors.initials: AB
authors.lastname: Pardee
authors.forename: L A
authors.initials: LA
authors.lastname: Beckett
authors.forename: C M
authors.initials: CM
authors.lastname: Ahlers
authors.forename: L
authors.initials: L
authors.lastname: Ecto
authors.forename: J
authors.initials: J
authors.lastname: Allen-Ryan
authors.forename: A
authors.initials: A
authors.lastname: Anisowicz
authors.forename: R
authors.initials: R
authors.lastname: Sager
authors.forename: C S
authors.initials: CS
authors.lastname: Crumpacker
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000235:genetics / Q000276:immunology*; D000199:Actins / Q000032:analysis; D015152:Blotting, Northern; D054360:Chemokine CXCL1; D019743:Chemokines, CXC; D002630:Chemotactic Factors / Q000096:biosynthesis / Q000235:genetics*; D015870:Gene Expression; D006133:Growth Substances / Q000096:biosynthesis / Q000235:genetics*; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins; D011336:Probability; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis / Q000235:genetics*
chemical_list: D000199:Actins; C475829:CXCL1 protein, human; D054360:Chemokine CXCL1; D019743:Chemokines, CXC; D002630:Chemotactic Factors; D006133:Growth Substances; D036341:Intercellular Signaling Peptides and Proteins; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha