Immunoelectron microscopic localization of antigens which react with islet cell cytoplasmic antibodies within human pancreatic beta cells.
The presence of circulating autoantibody to islet cell cytoplasm is considered to be an important marker of Type 1 (insulin-dependent) diabetes mellitus. In the present study using islet cell cytoplasmic antibody positive patient sera as the first antibody, we studied the intracellular distribution of its antigen at the electron microscopic level using the pre-embedding immunoperoxidase method. Specific immunoreactivity was found in the membranes of beta cell-secretory granules and cytoplasmic membranes. This result is compatible with the interpretation that the antigen(s) on the membranes of beta cell secretory granules is (are) the target of islet cell cytoplasmic antibody.
Takino
H
H
First Department of Internal Medicine, Nagasaki University School of Medicine, Japan.
Yoshimura
T
T
Kawasaki
E
E
Chikuba
N
N
Yokota
A
A
Akazawa
S
S
Tsujihata
M
M
Nakane
P K
PK
Nagataki
S
S
D001323:Autoantibodies; D001324:Autoantigens / Q000378:metabolism*; D002462:Cell Membrane / Q000276:immunology; D003594:Cytoplasmic Granules / Q000276:immunology; D006801:Humans; D007124:Immunoenzyme Techniques; D007425:Intracellular Membranes / Q000276:immunology; D007515:Islets of Langerhans / Q000276:immunology* / Q000648:ultrastructure; D016253:Microscopy, Immunoelectron
D001323:Autoantibodies; D001324:Autoantigens; C024211:islet cell antibody