Effect of intracarotid infusion of etoposide with angiotensin II-induced hypertension on the blood-brain barrier and the brain tissue.
This study investigated the effects of the intracarotid infusion of etoposide in combination with angiotensin II (AT II)-induced hypertension on the blood-brain barrier (BBB) and brain tissue in rats. Eighty rats were divided into five groups: Group 1, intravenous infusion of AT II to increase arterial blood pressure; Group 2, intracarotid infusion of etoposide at 22.5 mg/m2 for 10 minutes; Group 3, intracarotid infusion of etoposide at 75.0 mg/m2 for 10 minutes; Group 4, intracarotid infusion of etoposide at 75.0 mg/m2 for 20 minutes; Group 5, intracarotid infusion of etoposide at 75.0 mg/m2 for 10 minutes with AT II-induced hypertension. Evans blue staining of the brain was used as a monitor of BBB disruption. Mean arterial blood pressure over the experimental period in Group 1 increased from 86.3 +/- 1.3 mmHg (mean +/- SEM) to 139.0 +/- 2.4 mmHg, and Group 5 from 85.9 +/- 1.8 mmHg to 137.3 +/- 2.4 mmHg. None of the animals in Group 1 and 2 showed any obvious neurological change, while all the animals in Group 3, 4 and 5 exhibited diminished activity as their sole neurological change throughout the course of the experiment. Slight evidence of BBB disruption was seen in only 25% of the animals in Group 1. Significant BBB disruption was found in the animals in Group 2, 3, 4 and 5. No histological change was observed in any animal in Group 1 and 2.(ABSTRACT TRUNCATED AT 250 WORDS)
Ogasawara
H
H
Department of Neurosurgery, Hiroshima University School of Medicine, Japan.
Kiya
K
K
Kurisu
K
K
Hotta
T
T
Mikami
T
T
Sugiyama
K
K
Nakahara
T
T
Uozumi
T
T
D000804:Angiotensin II / Q000009:adverse effects* / Q000494:pharmacology; D000818:Animals; D001284:Atrophy; D001812:Blood-Brain Barrier / Q000187:drug effects*; D001921:Brain / Q000187:drug effects / Q000473:pathology; D001929:Brain Edema / Q000139:chemically induced* / Q000473:pathology; D002339:Carotid Arteries; D003711:Demyelinating Diseases / Q000139:chemically induced* / Q000473:pathology; D005047:Etoposide / Q000008:administration & dosage / Q000009:adverse effects* / Q000494:pharmacology; D005070:Evans Blue; D006973:Hypertension / Q000139:chemically induced / Q000503:physiopathology*; D007261:Infusions, Intra-Arterial; D008297:Male; D009336:Necrosis; D051381:Rats; D011916:Rats, Inbred F344
D000804:Angiotensin II; D005070:Evans Blue; D005047:Etoposide