title: Effects of loading on the synthesis of proteoglycans in different layers of anatomically intact articular cartilage in vitro.
abstract: The anatomically intact articular cartilage (approximately equal to 2.5 cm2) of whole bovine sesamoid bones was cultured on its bone support. A load of 5 kg was applied intermittently at 0.3 Hz by a specially designed loading apparatus for 7 days. [35S]-sulfate was added during the last 17 h of the experiment. Loading induced a 40% increase in [35S]-sulfate incorporation into aggrecans and an almost 3-fold increase in the synthesis of small proteoglycans. The loading effects occurred mainly in the upper half of the articular cartilage. Samples left unloaded for 7 days and then loaded for 7 days likewise showed an increase in [35S]-sulfate incorporation compared with unloaded controls.
authors.affiliation: Jan van Breemen Institut, Center for Rheumatology and Rehabilitation, Amsterdam, The Netherlands.
authors.forename: T H
authors.initials: TH
authors.lastname: Korver
authors.forename: R J
authors.initials: RJ
authors.lastname: van de Stadt
authors.forename: E
authors.initials: E
authors.lastname: Kiljan
authors.forename: G P
authors.initials: GP
authors.lastname: van Kampen
authors.forename: J K
authors.initials: JK
authors.lastname: van der Korst
mesh_terms: D000818:Animals; D002358:Cartilage, Articular / Q000166:cytology / Q000378:metabolism* / Q000502:physiology; D002417:Cattle; D006025:Glycosaminoglycans / Q000378:metabolism; D009924:Organ Culture Techniques; D011509:Proteoglycans / Q000096:biosynthesis* / Q000737:chemistry; D013314:Stress, Mechanical; D013455:Sulfur / Q000008:administration & dosage / Q000378:metabolism; D013462:Sulfur Radioisotopes; D013997:Time Factors
chemical_list: D006025:Glycosaminoglycans; D011509:Proteoglycans; D013462:Sulfur Radioisotopes; D013455:Sulfur