title: Extracellular brain glucose levels reflect local neuronal activity: a microdialysis study in awake, freely moving rats.
abstract: The relationship between brain extracellular glucose levels and neuronal activity was evaluated using microdialysis in awake, freely moving rats. The sodium channel blocker tetrodotoxin and the depolarizing agent veratridine were administered through the dialysis probe to provoke local changes in neuronal activity. The extracellular glucose content was significantly increased in the presence of tetrodotoxin and decreased sharply following veratridine application. The systemic injection of a general anaesthetic, chloral hydrate, led to a large and prolonged increase in extracellular glucose levels. The brain extracellular glucose concentration was estimated by comparing dialysate glucose efflux over a range of inlet glucose concentrations. A mean value of 0.47 mM was obtained in five animals. The results are discussed in terms of the coupling between brain glucose supply and metabolism. The changes observed in extracellular glucose levels under various conditions suggest that supply and utilization may be less tightly linked in the awake rat than has previously been postulated.
authors.affiliation: University Laboratory of Physiology, Oxford, England.
authors.forename: L K
authors.initials: LK
authors.lastname: Fellows
authors.forename: M G
authors.initials: MG
authors.lastname: Boutelle
authors.forename: M
authors.initials: M
authors.lastname: Fillenz
mesh_terms: D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D001923:Brain Chemistry; D002697:Chloral Hydrate / Q000494:pharmacology; D003243:Consciousness / Q000502:physiology*; D003956:Dialysis / Q000379:methods; D005110:Extracellular Space / Q000737:chemistry*; D005947:Glucose / Q000032:analysis* / Q000493:pharmacokinetics; D008297:Male; D009474:Neurons / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D051381:Rats; D017207:Rats, Sprague-Dawley; D013779:Tetrodotoxin / Q000494:pharmacology; D013997:Time Factors; D014701:Veratridine / Q000494:pharmacology
chemical_list: D002697:Chloral Hydrate; D013779:Tetrodotoxin; D014701:Veratridine; D005947:Glucose