title: [Electrophoretic studies of serum protein fractions in horses with laminitis].
abstract: The spectrum of serum proteins was evaluated in 46 horses affected with spontaneous laminitis and correlations between the severity of the disease and changes of the protein pattern were analyzed. The investigation was made in two groups; group A consisted of 21 horses of various breeds (warmblood, thoroughbred, standardbred) and group B of 25 ponys. Each group was subdivided according to the severity of the disease, using the OBEL-grade (OG) classification system. Serum proteins were separated by different one- and two-dimensional electrophoretic methods. Sera analysed by cellulose acetate electrophoresis showed a significant difference in the alpha 1-globulin fraction between OG II and OG IV affected horses. An increasing severity of the disease was correlated with a decrease of the alpha 1-globulins. The other protein fractions didn't show a uniform tendency. In group B there was a significant difference in the alpha 1-globulin fractions of OG II and OG III and in the beta 2-globulin fractions of OG I and OG II affected ponys. The acute phase proteins C3c, C4, Hp and fibronectin could be determined in a preliminary study in horse serum using the cross-reactivity of antibodies against the homologous human proteins.
authors.affiliation: Klinik für Orthopädie bei Huf- und Klauentieren, Veterinämedizinischen Universität Wien.
authors.forename: H
authors.initials: H
authors.lastname: Edinger
authors.forename: I
authors.initials: I
authors.lastname: Miller
authors.forename: C
authors.initials: C
authors.lastname: Stanek
authors.forename: M
authors.initials: M
authors.lastname: Gemeiner
mesh_terms: D000818:Animals; D001797:Blood Protein Electrophoresis / Q000662:veterinary; D001798:Blood Proteins / Q000737:chemistry*; D005260:Female; D005534:Foot Diseases / Q000097:blood / Q000662:veterinary; D006724:Hoof and Claw; D006734:Horse Diseases / Q000097:blood*; D006736:Horses; D008297:Male
chemical_list: D001798:Blood Proteins
vernacular_title: Elektrophoretische Untersuchungen der Serumproteinfraktionen hufrehekranker Pferde.