High-performance liquid chromatographic determination of angiotensin II receptor antagonists in human plasma and urine. I. DuP 532 (L-694,492).
A sensitive reversed-phase high-performance liquid chromatographic method with ultraviolet detection was developed for the analysis of a new angiotensin II receptor antagonist, DuP 532 (L-694,492), in human plasma and urine. The analyte and internal standard are extracted from plasma and urine at a pH between 3.3 to 3.6 by liquid-liquid extraction and analyzed on a C6 column with ultraviolet detection at 254 nm. The mobile phase is composed of acetonitrile and phosphate buffer at pH 2.5. The limits of quantification are 6 and 7.5 ng/ml for plasma and urine, respectively.
Chiou
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Department of Drug Metabolism, Merck Research Laboratories, West Point, PA 19486.
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D057911:Angiotensin Receptor Antagonists; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D007093:Imidazoles / Q000097:blood / Q000378:metabolism* / Q000652:urine; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet; D013777:Tetrazoles / Q000097:blood / Q000378:metabolism* / Q000652:urine; D005466:Fluorocarbons
D057911:Angiotensin Receptor Antagonists; D007093:Imidazoles; D013777:Tetrazoles; C069045:DuP 532; D005466:Fluorocarbons