title: Three-dimensional cell cultures mimic tissues.
abstract: Three-dimensional cell culture using gels of type I collagen is a flexible method for studying cell behavior in a tissuelike environment. With only small changes in the basic protocol, we were able to encapsulate neutrophils, hepatocytes, and PC12 cells. As demonstrated by cell-specific assays for migration, protein secretion, and growth factor induction, the encapsulated cells were viable and functional. In future studies, we will focus on using these cell cultures to study cell movement, cell growth, and cell function in carefully controlled tissuelike environments.
authors.affiliation: Department of Chemical Engineering, Johns Hopkins University, Baltimore, Maryland 21218.
authors.forename: W M
authors.initials: WM
authors.lastname: Saltzman
authors.forename: M R
authors.initials: MR
authors.lastname: Parkhurst
authors.forename: P
authors.initials: P
authors.lastname: Parsons-Wingerter
authors.forename: W H
authors.initials: WH
authors.lastname: Zhu
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D002455:Cell Division; D002478:Cells, Cultured; D002645:Chickens; D003470:Culture Media; D046508:Culture Techniques / Q000379:methods; D006801:Humans; D007700:Kinetics; D008099:Liver / Q000166:cytology* / Q000502:physiology; D009414:Nerve Growth Factors / Q000096:biosynthesis; D009504:Neutrophils / Q000166:cytology*; D016716:PC12 Cells; D012709:Serum Albumin / Q000096:biosynthesis; D013997:Time Factors
chemical_list: D003470:Culture Media; D009414:Nerve Growth Factors; D012709:Serum Albumin