title: Ring test for low levels of N-(2-hydroxyethyl)valine in human hemoglobin.
abstract: Hemoglobin adducts are useful for the identification and quantification of electrophilic agents in vivo. A modified Edman degradation method has been extensively used for monitoring exposure to ethylene oxide through gas chromatographic-mass spectrometric measurements of hydroxyethyl adducts to the N-terminal valines in hemoglobin. In a ring test, four laboratories using different versions of the method analyzed eight human globin samples with low adduct levels from ethylene oxide. Measurements of the same adduct by a radioimmunoassay were also included. Strong correlation between the measurements by the different laboratories shows that the method in principle works well. However, there were some systematic quantitative differences.
authors.affiliation: Department of Radiobiology, Stockholm University, Sweden.
authors.forename: M
authors.initials: M
authors.lastname: Törnqvist
authors.forename: A L
authors.initials: AL
authors.lastname: Magnusson
authors.forename: P B
authors.initials: PB
authors.lastname: Farmer
authors.forename: Y S
authors.initials: YS
authors.lastname: Tang
authors.forename: A M
authors.initials: AM
authors.lastname: Jeffrey
authors.forename: L
authors.initials: L
authors.lastname: Wazneh
authors.forename: G D
authors.initials: GD
authors.lastname: Beulink
authors.forename: H
authors.initials: H
authors.lastname: van der Waal
authors.forename: N J
authors.initials: NJ
authors.lastname: van Sittert
mesh_terms: D006454:Hemoglobins / Q000737:chemistry*; D006801:Humans; D011863:Radioimmunoassay; D012044:Regression Analysis; D014633:Valine / Q000031:analogs & derivatives* / Q000032:analysis
chemical_list: D006454:Hemoglobins; C057442:2-hydroxyethylvaline; D014633:Valine