title: [Construction of linear plasmid vectors for cloning in Escherichia coli cells].
abstract: Prophage of the temperate coliphage N15 is a linear plasmid with covalently closed ends. The central part of plasmid N15 genome responsible for vegetative phage growth was replaced by DNA fragments containing genes for selective markers which have unique restriction sites. As a result a family of linear plasmid vectors was constructed. Their size is about 20 kb and their capacity is comparable with that of cosmid vectors.
authors.forename: A A
authors.initials: AA
authors.lastname: Vostrov
authors.forename: A Iu
authors.initials: AIu
authors.lastname: Malinin
authors.forename: V N
authors.initials: VN
authors.lastname: Rybchin
authors.forename: A N
authors.initials: AN
authors.lastname: Sravchevskiĭ
mesh_terms: D001435:Bacteriophages / Q000235:genetics; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics*; D005822:Genetic Vectors / Q000235:genetics; D010957:Plasmids / Q000235:genetics*; D015183:Restriction Mapping
vernacular_title: Konstruirovanie lineĭnykh plazmidnykh vektorov dlia klonirovaniia v kletkakh Escherichia coli.