Alw26I, Eco31I and Esp3I--type IIs methyltransferases modifying cytosine and adenine in complementary strands of the target DNA.
The specificity of three DNA methyltransferases M.Alw26I, M.Eco31I and M.Esp3I, isolated from Acinetobacter Iwoffi RFL26, Escherichia coli RFL31 and Hafnia alvei RFL3+, respectively, was determined. All the enzymes methylate both strands of asymmetric recognition sites yielding m5C in the top-strand and m6A in the bottom-strand, as below: 5'-GTm5CTC 5'-GGTm5CTC 5'-CGTm5CTC 3'-Cm6AGAG 3'-CCm6AGAG 3'-GCm6AGAG (M.Alw26I) (M.Eco31I) (M.Esp3I) They are the first members of type IIs methyltransferases that modify different types of nucleotides in the recognition sequence.
Bitinaite
J
J
Institute of Biotechnology FERMENTAS, Vilnius, Lithuania.
Maneliene
Z
Z
Menkevicius
S
S
Klimasauskas
S
S
Butkus
V
V
Janulaitis
A
A
D000150:Acinetobacter / Q000201:enzymology*; D001483:Base Sequence; D015254:DNA Modification Methylases / Q000378:metabolism*; D015257:DNA-Cytosine Methylases / Q000378:metabolism*; D004755:Enterobacteriaceae / Q000201:enzymology*; D004926:Escherichia coli / Q000201:enzymology*; D007700:Kinetics; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000378:metabolism; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific) / Q000378:metabolism*; D013379:Substrate Specificity
D009838:Oligodeoxyribonucleotides; D015254:DNA Modification Methylases; C077513:DNA modification methylase Alw26I; C077514:DNA modification methylase Eco31I; C077515:DNA modification methylase Esp3I; D015257:DNA-Cytosine Methylases; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific)