title: High-performance liquid chromatographic-mass spectrometric assay of busulfan in serum and cerebrospinal fluid.
abstract: A liquid chromatographic-mass spectrometric method has been developed to determine busulfan concentrations in the cerebrospinal fluid and serum of some children undergoing bone marrow autotransplantation. After two liquid-liquid extraction steps with dichloromethane on a biological matrix, the separation of busulfan was carried out by isocratic reversed-phase chromatography. The mass spectrometric system was operated in electron-impact mode. Principal ions at m/z 175, 111 and 79 were observed for busulfan, but only m/z 175 was chosen for the quantification of the analyte. The retention time of busulfan was 2.5 min. The detection limit of 100 ng/ml allowed the determination of cerebrospinal fluid and serum busulfan concentrations during the four days of high-dose (1 mg/kg) treatment prior to autotransplantation in five child patients.
authors.affiliation: Istituto Superiore di Sanita, Rome, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Pichini
authors.forename: I
authors.initials: I
authors.lastname: Altieri
authors.forename: A
authors.initials: A
authors.lastname: Bacosi
authors.forename: S
authors.initials: S
authors.lastname: Di Carlo
authors.forename: P
authors.initials: P
authors.lastname: Zuccaro
authors.forename: P
authors.initials: P
authors.lastname: Iannetti
authors.forename: R
authors.initials: R
authors.lastname: Pacifici
mesh_terms: D002066:Busulfan / Q000032:analysis* / Q000097:blood / Q000134:cerebrospinal fluid; D002138:Calibration; D002648:Child; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000097:blood / Q000134:cerebrospinal fluid; D013058:Mass Spectrometry / Q000379:methods*; D015203:Reproducibility of Results
chemical_list: D002066:Busulfan