title: Effect of food restriction on the phosphocreatine energy shuttle components in rat heart.
abstract: Malnutrition has been associated with changes in cardiac metabolism and performance. We have previously reported a diabetic-type cardiomyopathy associated with chronic food restriction and weight loss. Because the creatine-phosphocreatine-creatine kinase system is important in the contractile process, we studied the components of this system in rats fed a food-restricted diet (33% of control animal intake). After 4 weeks of food restriction, total creatine kinase (CK) activities were reduced by 28% in ventricles and by 38% in atria. The CK isoenzymes in the heart were not equally affected. The BB isoenzyme was decreased by 77% and 78%, the MB isoenzyme by 45% and 43%, the MM isoenzyme by 22% and 19% and CKmito by 16% and 15% in ventricles and atria, respectively. In contrast, brain CK activity which is predominantly the BB isoenzyme, was slightly higher in the food-restricted than in control rats. Further studies on ventricular tissue from food-restricted rats revealed a 27% decline in myofibrillar CR activity and a 58% decline in myofibrillar ATPase activity. Phosphocreatine and creatine concentrations were not changed by food restriction, however, ATP was decreased by 23% in ventricles from rats on the restricted diet. Cardiac mitochondrial oxidative phosphorylation was also impaired. State 3 respiration with alpha-ketoglutarate was reduced 20% in the food-restricted heart. These changes are compared to those which we previously observed in the diabetic rat heart and the significance of these findings is discussed.
authors.affiliation: Department of Pharmacology and Nutrition, University of Southern California, School of Medicine, Los Angeles 90033.
authors.forename: A
authors.initials: A
authors.lastname: Kirsch
authors.forename: F
authors.initials: F
authors.lastname: Savabi
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism; D000263:Adenylate Kinase / Q000378:metabolism; D000818:Animals; D003402:Creatine Kinase / Q000378:metabolism; D004734:Energy Metabolism; D005508:Food Deprivation / Q000502:physiology*; D007527:Isoenzymes; D008297:Male; D008929:Mitochondria, Heart / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism*; D009748:Nutrition Disorders / Q000378:metabolism; D010085:Oxidative Phosphorylation; D010725:Phosphocreatine / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D007527:Isoenzymes; D010725:Phosphocreatine; D003402:Creatine Kinase; D000263:Adenylate Kinase; D000251:Adenosine Triphosphatases