[Cellular mechanisms of impaired cardiac energetics in patients with dilated cardiomyopathy: decrease in mitochondrial respiration and creatine kinase expression].
The mitochondrial functional characteristics were assessed in the biopsy specimens from patients with various Functional Classes dilated cardiomyopathy (DCMP). The assessment was made by using endomyocardial biopsy specimens weighing 2-4 mg which had been taken from 39 patients aged 19-64 years during coronary ventriculography and cardiac transplantation. The status of mitochondria and the efficiency of mitochondrial creatine kinase functioning were evaluated by recording the respiration of saponin-skinned muscular fibers. The maximum mitochondrial respiration rate calculated on a dry weight basis was not substantially different in all functional classes of DCMP, while the acceptor control index (Vmax/V0) and the level of creatine-activated respiration decreased with an increase in the functional class of DCMP. The findings show a good positive correlation between ejection fraction and creatine-stimulated respiration values and a linear negative correlation between this parameter and end-diastolic pressures. Thus, the respiratory parameters of mitochondria in the endomyocardial biopsy specimens may be used to assess the severity of cardias lesions.
Branishte
T A
TA
Kuznetzov
A V
AV
Khuchua
Z A
ZA
Samko
A N
AN
Naumov
V G
VG
Semenovskiĭ
M L
ML
Belenkov
Iu N
IuN
Saks
V A
VA
D000328:Adult; D002311:Cardiomyopathy, Dilated / Q000201:enzymology / Q000378:metabolism*; D003402:Creatine Kinase / Q000378:metabolism*; D015870:Gene Expression; D006801:Humans; D008875:Middle Aged; D008929:Mitochondria, Heart / Q000201:enzymology / Q000378:metabolism*; D010101:Oxygen Consumption / Q000502:physiology*
D003402:Creatine Kinase
Kletochnye mekhanizmy narusheniia énergetiki serdtsa u bol'nykh s dilatatsionnoĭ kardiomiopatieĭ: umen'shenie mitokhondrial'nogo dykhaniia i ékspressii kreatinkinazy.