title: Effect of hydrogen peroxide on mammalian chromatin: electron microscopic studies.
abstract: Chromatins purified from rat liver as well as from cultured Chinese hamster V79 cells were treated with hydrogen peroxide in presence of iron in vitro. When examined in a transmission electron microscope, chromatins from both the sources were found to lose the native morphology. There were evidences of aggregation of the chromatin fibers. This could be due to DNA-protein and DNA interstrand crosslinking induced by Fenton reaction.
authors.affiliation: Crystallography and Molecular Biology Division, Saha Institute of Nuclear Physics, Calcutta, India.
authors.forename: S
authors.initials: S
authors.lastname: Chaudhuri
authors.forename: S
authors.initials: S
authors.lastname: Sengupta
authors.forename: N P
authors.initials: NP
authors.lastname: Bhattacharyya
authors.forename: S B
authors.initials: SB
authors.lastname: Bhattacharjee
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002843:Chromatin / Q000737:chemistry / Q000187:drug effects* / Q000302:isolation & purification / Q000648:ultrastructure*; D006224:Cricetinae; D003412:Cricetulus; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D008297:Male; D008854:Microscopy, Electron; D051381:Rats
chemical_list: D002843:Chromatin; D006861:Hydrogen Peroxide