Comparison of the ECL-cell frequency in the stomachs of 3 different rat strains.
Three different rat strains, Sprague-Dawley, Wistar and Fischer 344, were treated for 3 months with 2 doses (0.8; 4 mg/kg) of the gastric acid suppressing ATPase inhibitor pantoprazole. The gastrin levels were determined, the height of the mucosa measured and the number of enterochromaffin-like (ECL) cells counted. Because these cells were stained according to the method of Grimelius they were designated as GPC (Grimelius positive cells). Under 4 mg/kg, the gastrin levels were increased 8 hours after administration, but fell again after 24 h. The Fischer rats showed the highest value. Also the height of the mucosa was increased under 4 mg/kg. A trend towards an increased mucosal height was noticeable even at 0.8 mg/kg. The number of GPC was determined in 2 ways: 1) without taking the mucosal height into account, 2) taking the height into account. An increase in GPC was observed at 4 mg/kg with both methods.
Tuch
K
K
Byk Gulden Pharmaceuticals, Institute for Pathology and Toxicology, Hamburg, FRG.
Ockert
D
D
Hauschke
D
D
Christ
B
B
D053799:2-Pyridinylmethylsulfinylbenzimidazoles; D000251:Adenosine Triphosphatases / Q000037:antagonists & inhibitors; D000284:Administration, Oral; D000818:Animals; D001562:Benzimidazoles / Q000008:administration & dosage / Q000494:pharmacology; D002452:Cell Count; D004759:Enterochromaffin Cells / Q000737:chemistry / Q000166:cytology*; D005260:Female; D005753:Gastric Mucosa / Q000737:chemistry / Q000166:cytology / Q000187:drug effects; D005755:Gastrins / Q000032:analysis; D007091:Image Processing, Computer-Assisted; D009853:Omeprazole / Q000031:analogs & derivatives; D000077402:Pantoprazole; D051381:Rats; D011916:Rats, Inbred F344; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D013045:Species Specificity; D013270:Stomach / Q000737:chemistry / Q000166:cytology* / Q000187:drug effects; D013454:Sulfoxides / Q000008:administration & dosage / Q000494:pharmacology
D053799:2-Pyridinylmethylsulfinylbenzimidazoles; D001562:Benzimidazoles; D005755:Gastrins; D013454:Sulfoxides; D000077402:Pantoprazole; D000251:Adenosine Triphosphatases; D009853:Omeprazole