Monomer diffusion and polymer alignment in domains of sickle hemoglobin.
We have used polarized absorbance to observe the process of monomer accretion and polymer alignment which occurs in domains of sickle hemoglobin that are formed and maintained by laser photolysis. These diffusion and alignment processes have been studied as a function of initial concentration and temperature (initial and final), as well as beam size and domain number. Monomers are found to diffuse into growing polymer domains with a rate that is essentially temperature and concentration independent, but which depends on the size of the final domain boundaries, and the number of domains within a boundary. The final concentrations achieved are very close to those found in packed centrifugation experiments (50-55 g/dl) and are approximately independent of starting temperature and concentration. The influx of monomers is accompanied by polymer alignment, and the amount aligned is proportional to the amount diffused throughout the process. We propose that polymer alignment controls the influx of added monomers into the growing domain.
Cho
M R
MR
Department of Physics and Atmospheric Science, Drexel University, Philadelphia, Pennsylvania 19104.
Ferrone
F A
FA
D055592:Biophysical Phenomena; D001703:Biophysics; D006451:Hemoglobin, Sickle / Q000737:chemistry*; D006801:Humans; D007700:Kinetics; D008027:Light; D008956:Models, Chemical; D011108:Polymers / Q000737:chemistry; D011487:Protein Conformation; D012542:Scattering, Radiation; D013053:Spectrophotometry; D013696:Temperature
D006451:Hemoglobin, Sickle; D011108:Polymers