Effects of antibiotics and other drugs on toxin production in Clostridium difficile in vitro and in vivo.
In an attempt to understand more completely why patients treated with phenothiazines (chlorpromazine and cyamemazine), methotrexate, and certain antibiotics such as clindamycin have an increased risk of developing pseudomembranous colitis, the production of toxins A and B by Clostridium difficile in the presence of these drugs was measured in vitro as well as in vivo by using axenic mice. None of the drugs tested increased the production of toxins either in vitro or in vivo.
Barc
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MC
Laboratoire de Microbiologie, Faculté de Pharmacie, Centre d'Etudes Pharmaceutiques, Châtenay-Malabry, France.
Depitre
C
C
Corthier
G
G
Collignon
A
A
Su
W J
WJ
Bourlioux
P
P
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins; D001427:Bacterial Toxins / Q000096:biosynthesis*; D016466:CHO Cells; D016360:Clostridioides difficile / Q000187:drug effects / Q000378:metabolism*; D003015:Clostridium Infections / Q000378:metabolism / Q000382:microbiology; D006224:Cricetinae; D004768:Enterotoxins / Q000096:biosynthesis*; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000737:chemistry / Q000382:microbiology; D051379:Mice; D008809:Mice, Inbred C3H
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004768:Enterotoxins; C041348:tcdA protein, Clostridium difficile; C031815:toxB protein, Clostridium difficile