Effect of adenine metabolites on survival of Drosophila melanogaster of low xanthine dehydrogenase activity.
1. Low xanthine dehydrogenase (LXD) mutant Drosophila melanogaster were fed 0.2% adenine for 7 generations, no adenine for the next 2 generations (relaxed) and 0.2% adenine again for the next 3 generations (rechallenged) to obtain adenine-resistant lines of Drosophila (LXD-adenine). Flies grown without adenine served as LXD-controls. 2. Purines ranked as follows; adenine > adenosine > AMP > inosine > IMP in decreasing order of toxicity to LXD-adenine flies. 3. Addition of ribose to 9N position, or phosphate or carboxy to 6C position of the purine ring alleviated the toxicity. 4. More LXD-adenine offspring survived than did LXD-control offspring rechallenged with adenine.
Ho
Y K
YK
Department of Biology, City College of San Francisco, CA 94112.
Guthrie
M J
MJ
Clifford
A J
AJ
Ho
C C
CC
D000225:Adenine / Q000378:metabolism / Q000494:pharmacology* / Q000633:toxicity; D000818:Animals; D004331:Drosophila melanogaster / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D005260:Female; D008297:Male; D009154:Mutation; D011686:Purine-Pyrimidine Metabolism, Inborn Errors / Q000235:genetics / Q000378:metabolism; D011687:Purines / Q000378:metabolism / Q000494:pharmacology / Q000633:toxicity; D013329:Structure-Activity Relationship; D014968:Xanthine Dehydrogenase / Q000172:deficiency* / Q000235:genetics
D011687:Purines; D014968:Xanthine Dehydrogenase; D000225:Adenine