title: Intrinsic radiosensitivity of normal human fibroblasts and lymphocytes after high- and low-dose-rate irradiation.
abstract: The existence of heritable radiosensitivity syndromes and clinical observations in radiotherapy patients suggests that human cellular radiosensitivity differs among individuals. We report here an in vitro study of radiosensitivity in 30 fibroblast and 29 lymphocyte cultures obtained from cancer patients and controls. In 25 cases, both fibroblasts and lymphocytes were obtained from the same donors. Fibroblasts were cultured from skin biopsy samples, and peripheral T-cell lymphocytes were cultured from blood. Clonogenic survival assays were performed by using high- and low-dose-rate irradiation; lymphocytes were in G0 phase and fibroblasts in confluent plateau phase. Various end points were calculated and compared (i.e., surviving fraction at 2 Gy, initial slope of the survival curve, and doses resulting in 10 and 1% survival, respectively). Depending on the end point, the coefficient of variation of the survival parameters ranged from 31 to 68% for lymphocytes and 21 to 41% for fibroblasts following high-dose-rate irradiation. Similar ranges were obtained after low-dose-rate irradiation. Variance analysis performed on replicate assays in cultures derived from the same patient showed that variation due to technical or sampling errors was significantly lower than variation between individuals (P = 0.00034 and 0.014 for fibroblasts and lymphocytes, respectively). No correlation was observed between the radiosensitivity of lymphocyte and fibroblast cultures derived from the same donors. We conclude that there is significant variation in normal cell radiosensitivity among individuals. On the other hand, comparisons of lymphocyte and fibroblast radiosensitivities suggest that tissue-specific characteristics, such as differentiation status, may variably modulate radiosensitivity.
authors.affiliation: Department of Experimental Radiotherapy, University of Texas, M. D. Anderson Cancer Center, Houston 77030.
authors.forename: F B
authors.initials: FB
authors.lastname: Geara
authors.forename: L J
authors.initials: LJ
authors.lastname: Peters
authors.forename: K K
authors.initials: KK
authors.lastname: Ang
authors.forename: J L
authors.initials: JL
authors.lastname: Wike
authors.forename: S S
authors.initials: SS
authors.lastname: Sivon
authors.forename: R
authors.initials: R
authors.lastname: Guttenberger
authors.forename: D L
authors.initials: DL
authors.lastname: Callender
authors.forename: E P
authors.initials: EP
authors.lastname: Malaise
authors.forename: W A
authors.initials: WA
authors.lastname: Brock
mesh_terms: D001943:Breast Neoplasms / Q000097:blood / Q000473:pathology; D002470:Cell Survival / Q000528:radiation effects; D002478:Cells, Cultured; D004307:Dose-Response Relationship, Radiation; D005347:Fibroblasts / Q000166:cytology / Q000528:radiation effects*; D014644:Genetic Variation / Q000502:physiology; D006258:Head and Neck Neoplasms / Q000097:blood / Q000473:pathology; D006801:Humans; D011836:Radiation Tolerance / Q000235:genetics*; D012867:Skin / Q000166:cytology / Q000528:radiation effects; D013601:T-Lymphocytes / Q000166:cytology / Q000528:radiation effects*