Recombinant parvovirus B19 capsids as a new substrate for detection of B19-specific IgG and IgM antibodies by an enzyme-linked immunosorbent assay.
A new enzyme-linked immunosorbent assay for the detection of B19-specific IgG and IgM antibodies was established using B19 capsids synthesized in a baculovirus expression system. These B19 capsids, consisting of either coat protein VP2 alone or of both VP1 and VP2, have been shown to be similar to native virus in size and appearance. The results obtained for the detection of B19-specific antibodies showed good correlations with a radioimmunoassay which uses native B19 virus and an immunofluorescence assay based on insect cells expressing coat protein VP1. The course of the antibody response could be followed by determining the titers of sequential serum samples taken after a recent B19 infection. Both types of recombinant capsids form an excellent source of antigen for the detection of both B19 IgG and IgM antibodies and are a very promising substitute for native virus.
Salimans
M M
MM
Department of Virology, University Hospital Leiden, The Netherlands.
van Bussel
M J
MJ
Brown
C S
CS
Spaan
W J
WJ
D000914:Antibodies, Viral / Q000096:biosynthesis / Q000097:blood*; D002213:Capsid / Q000097:blood / Q000235:genetics* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D016731:Erythema Infectiosum / Q000382:microbiology; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood*; D007075:Immunoglobulin M / Q000097:blood*; D016732:Parvovirus B19, Human / Q000235:genetics* / Q000276:immunology; D011994:Recombinant Proteins / Q000097:blood / Q000276:immunology; D013379:Substrate Specificity
D000914:Antibodies, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D011994:Recombinant Proteins