Capillary electrophoretic determination of the protease Savinase in cultivation broth.
The highly basic washing enzyme Savinase and various analogues were analysed by micellar electrokinetic chromatography (MEKC) and electrophoresis. Broth samples were withdrawn during the cultivation of Savinase by recombinant microorganisms. Savinase peak areas obtained by MEKC-electrophoretic analysis were normalized with respect to migration time and compared with traditional enzyme activity measurements. The electropherograms indicated thermal degradation of the Savinase molecule at high field strengths. Baseline separation of Savinase and two analogues was achieved.
Vinther
A
A
Novo Nordisk A/S, Bagsvaerd, Denmark.
Petersen
J
J
Søeberg
H
H
D001407:Bacillus / Q000201:enzymology; D003470:Culture Media / Q000032:analysis; D004586:Electrophoresis; D006358:Hot Temperature; D007525:Isoelectric Focusing; D012697:Serine Endopeptidases / Q000032:analysis* / Q000096:biosynthesis
D003470:Culture Media; D012697:Serine Endopeptidases; C032923:microbial serine proteinases