title: Characterization of rabies virus glycoprotein expressed by recombinant baculovirus.
abstract: A cDNA of the glycoprotein (G protein) gene of rabies virus Nishigahara strain was cloned and inserted into a baculovirus genome under the control of the polyhedrin promoter. Infection of Spodoptera frugiperda cells with this recombinant virus produced a large quantity of new protein instead of the parental polyhedrin protein. By immunofluorescent and immunoblotting analyses, the recombinant protein was antigenically similar to the authentic G protein. Its molecular mass estimated by sodium dodecyl sulfate polyacrylamide gel electrophoresis, however, was slightly smaller than that of the authentic one, and this observation was suggested to be due to the difference in glycosylation level between the two G proteins. The recombinant G protein expressed on the cell surface of the insect cells showed a fusion activity at low pH. The fusion activity was inhibited by antiserum against either whole virions or G protein of rabies virus.
authors.affiliation: Nippon Institute for Biological Science, Tokyo, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Tuchiya
authors.forename: Y
authors.initials: Y
authors.lastname: Matsuura
authors.forename: A
authors.initials: A
authors.lastname: Kawai
authors.forename: A
authors.initials: A
authors.lastname: Ishihama
authors.forename: S
authors.initials: S
authors.lastname: Ueda
mesh_terms: D000818:Animals; D016367:Baculoviridae / Q000235:genetics; D001483:Base Sequence; D002478:Cells, Cultured; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000235:genetics; D005822:Genetic Vectors; D006023:Glycoproteins / Q000737:chemistry / Q000235:genetics*; D006031:Glycosylation; D008969:Molecular Sequence Data; D011820:Rabies virus / Q000235:genetics*; D011994:Recombinant Proteins / Q000235:genetics; D014760:Viral Fusion Proteins / Q000737:chemistry / Q000235:genetics*
chemical_list: D004279:DNA, Viral; D006023:Glycoproteins; D011994:Recombinant Proteins; D014760:Viral Fusion Proteins