Comparative lectinhistochemical studies on paraffin- and glycol methacrylate-embedded CNS tissue specimens from AIDS autopsies. Mistletoe lectin I (ML I) as cell-marker.
Brain tissue and spinal cord tissue from 12 patients who had died of AIDS was fixed in neutral formalin; then after the embedment of some of it in paraffin and some of it in glycol methacrylate, it was analyzed lectinhistochemically with mistletoe lectin I (ML I). Mistletoe lectin (ML I) is a reliable marker for microglia cells and macrophages and for special cell forms (polynuclear giant cells, so-called pericytes) belonging to this cell system. In both the embedding procedures used, the representation of the cells is very clear and subtly differentiated so that the preparations are very well suited to the study of AIDS-associated tissue damage in the CNS. Whereas the immunological macrophage markers (CD 68 markers) KPI, PG-M1 produce good results on paraffin-embedded material but are completely useless on material embedded in glycol methacrylate, the cells of the monocyte-macrophage system can be represented very well and reliably after being embedded in glycol methacrylate with mistletoe lectin I (ML I).
Artigas
J
J
Institut für Pathologie, Auguste-Viktoria-Krankenhaus Berlin-Schöneberg, Deutschland.
Bachler
B
B
Habedank
S
S
Taube
F
F
Franz
H
H
Niedobitek
F
F
D015526:AIDS Dementia Complex / Q000473:pathology; D000163:Acquired Immunodeficiency Syndrome / Q000150:complications / Q000473:pathology*; D001344:Autopsy; D015415:Biomarkers; D001921:Brain / Q000473:pathology*; D001927:Brain Diseases / Q000209:etiology / Q000473:pathology*; D002241:Carbohydrates / Q000032:analysis; D006652:Histological Techniques; D006801:Humans; D007150:Immunohistochemistry; D037102:Lectins; D008689:Methacrylates; D014784:Mistletoe; D010232:Paraffin; D037121:Plant Lectins; D028321:Plant Preparations; D010940:Plant Proteins; D010946:Plants, Medicinal; D054790:Ribosome Inactivating Proteins, Type 2; D014118:Toxins, Biological
D015415:Biomarkers; D002241:Carbohydrates; D037102:Lectins; D008689:Methacrylates; D037121:Plant Lectins; D028321:Plant Preparations; D010940:Plant Proteins; D054790:Ribosome Inactivating Proteins, Type 2; D014118:Toxins, Biological; C488862:mistletoe lectin I; C009674:ribosome inactivating protein, Viscum; C005044:hydroxyethyl methacrylate; D010232:Paraffin