Detection of uremic peaks in dogs by anion exchange high performance liquid chromatography.
Sera of dogs with gentamicin-induced uremia were analyzed by high performance liquid chromatography system with strongly basic macroreticular anion exchange resin. Satisfactory separation of peaks was achieved with good reproducibility after deproteinization of sera with trichloroacetic acid at a final concentration of 3%, confirming that the system was suitable for qualitative analysis of uremic serum. The chromatograms showed that the number of peaks and the peak area had relation to concentrations of serum urea nitrogen or creatinine and severity of uremia. Four peaks were selected as suspected canine uremic peaks with high correlation to serum creatinine concentrations which were hardly influenced by extrarenal factors. The results suggested that these four fractions might contain uremic toxins.
Kawamura
M
M
Department of Veterinary Surgery, Faculty of Agriculture, University of Tokyo, Japan.
Ohashi
F
F
Murakami
F
F
Takai
N
N
Nishimura
R
R
Sasaki
N
N
Takeuchi
A
A
D000818:Animals; D000837:Anion Exchange Resins; D002851:Chromatography, High Pressure Liquid / Q000379:methods / Q000662:veterinary*; D004283:Dog Diseases / Q000097:blood* / Q000139:chemically induced; D004285:Dogs; D005260:Female; D005839:Gentamicins; D008297:Male; D015203:Reproducibility of Results; D014511:Uremia / Q000097:blood / Q000139:chemically induced / Q000662:veterinary*
D000837:Anion Exchange Resins; D005839:Gentamicins