title: Oxidized HDL are much less cytotoxic to lymphoblastoid cells than oxidized LDL.
abstract: The possible effect of oxidized HDL was investigated on lymphoblastoid cells, in comparison to the cytotoxic effect of oxidized LDL. Oxidation of HDL was promoted by UV-C irradiation, or by copper ion (5 microM) or the combination of the two treatments. HDL extensively treated by UV-C for 20 h did not exhibit any cytotoxic effect on cultured lymphoblastoid cells even at a concentration of 500 micrograms apolipoprotein A-I/ml. In contrast to UV-treated (2 h) LDL, which were highly cytotoxic (already at a concentration of 100 micrograms apolipoprotein B/ml), HDL treated by copper or copper + UV were oxidized, as shown by TBARS formation and PUFA content decrease, but were slightly cytotoxic.
authors.affiliation: Laboratoire de Biochimie, Université Paul Sabatier, Faculté de Médecine Rangueil, Toulouse, France.
authors.forename: Y
authors.initials: Y
authors.lastname: Alomar
authors.forename: A
authors.initials: A
authors.lastname: Nègre-Salvayre
authors.forename: T
authors.initials: T
authors.lastname: Levade
authors.forename: P
authors.initials: P
authors.lastname: Valdiguié
authors.forename: R
authors.initials: R
authors.lastname: Salvayre
mesh_terms: D001161:Arteriosclerosis / Q000209:etiology; D002460:Cell Line / Q000187:drug effects; D003300:Copper; D019327:Copper Sulfate; D005231:Fatty Acids, Unsaturated / Q000032:analysis; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000032:analysis; D008075:Lipoproteins, HDL / Q000737:chemistry / Q000633:toxicity*; D008077:Lipoproteins, LDL / Q000737:chemistry / Q000633:toxicity*; D010084:Oxidation-Reduction; D013778:Tetrazolium Salts; D013844:Thiazoles; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis; D014343:Trypan Blue; D014466:Ultraviolet Rays
chemical_list: D005231:Fatty Acids, Unsaturated; D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL; D013778:Tetrazolium Salts; D013844:Thiazoles; D017392:Thiobarbituric Acid Reactive Substances; D003300:Copper; D007770:L-Lactate Dehydrogenase; C022616:thiazolyl blue; D014343:Trypan Blue; D019327:Copper Sulfate