Comparative effects of halothane associated with verapamil and ischaemia on myocardial metabolism in isolated perfused rat hearts.
The association of verapamil with halothane causes ischaemic-like myocardial dysfunction. Using an isolated rat heart model perfused with a radiolabelled fatty acid (123I-labelled iodohexadecenoic acid) as a sensitive marker of ischaemia this study investigated whether or not this dysfunction is of ischaemic origin. Hearts were perfused with a control solution or with solutions containing either 1% of halothane or 150 ng ml-1 of verapamil or the association of 0.75% halothane + 120 ng ml-1 verapamil. The ischaemic group was perfused at a reduced perfusion rate (-50%). Intracellular fate of IHA was assessed, and its esterification ratio computed. Ischaemia and the drugs induced a similar depression of haemodynamics. The esterification ratio in the ischaemic group was significantly higher (0.723 +/- 0.04) than in controls (0.0526 +/- 0.03) and than in the treated groups: halothane (0.533 +/- 0.06), verapamil (0.411 +/- 0.027) or the association halothane+verapamil (0.408 +/- 0.05), suggesting a non-ischaemic origin for the dysfunction caused by halothane-verapamil.
Arvieux
C C
CC
Département d'Anesthésie Réanimation, Université J. Fourier, C.H.U de Grenoble, France.
Pernin
C
C
Drouet
N
N
Mathieu
J P
JP
Hamant
S
S
Dubois
F
F
Cuchet
P
P
Stieglitz
P
P
Comet
M
M
D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D004338:Drug Combinations; D006221:Halothane / Q000008:administration & dosage / Q000494:pharmacology*; D006339:Heart Rate / Q000187:drug effects; D007457:Iodine Radioisotopes; D008055:Lipids / Q000032:analysis; D008297:Male; D017202:Myocardial Ischemia / Q000378:metabolism* / Q000503:physiopathology; D009206:Myocardium / Q000737:chemistry / Q000378:metabolism*; D010084:Oxidation-Reduction; D010169:Palmitic Acids / Q000378:metabolism / Q000493:pharmacokinetics; D010477:Perfusion; D051381:Rats; D017208:Rats, Wistar; D016277:Ventricular Function, Left / Q000187:drug effects; D014700:Verapamil / Q000008:administration & dosage / Q000494:pharmacology*
D004338:Drug Combinations; D007457:Iodine Radioisotopes; D008055:Lipids; D010169:Palmitic Acids; C040433:16-iodo-9-hexadecenoic acid; D014700:Verapamil; D006221:Halothane