title: Complementation of Saccharomyces cerevisiae acid phosphatase mutation by a genomic sequence from the yeast Yarrowia lipolytica identifies a new phosphatase.
abstract: A Yarrowia lipolytica gene library was constructed in vector YRp7 and transformed into a Saccharomyces cerevisiae strain lacking both major acid phosphatase activities. A 2.18 kb genomic sequence restoring the ability to hydrolyze alpha-naphthyl phosphate was isolated. Its sequencing revealed an ORF encoding 358 amino acids without significant homology with any known phosphatase. A putative signal peptide and several possible sites for N-glycosylation were identified. Phosphate-regulated expression of the cloned gene was observed in Y. lipolytica. Disruption data favoured the hypothesis that it might encode a minor phosphatase species.
authors.affiliation: INRA, Centre de Biotechnologies Agro-Industrielles, INA-PG, Thiverval-Grignon, France.
authors.forename: B Y
authors.initials: BY
authors.lastname: Tréton
authors.forename: M T
authors.initials: MT
authors.lastname: Le Dall
authors.forename: C M
authors.initials: CM
authors.lastname: Gaillardin
mesh_terms: D000135:Acid Phosphatase / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004271:DNA, Fungal; D015723:Gene Library; D005800:Genes, Fungal; D005816:Genetic Complementation Test; D006031:Glycosylation; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010957:Plasmids; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics; D004718:Saccharomycetales / Q000201:enzymology* / Q000235:genetics; D014170:Transformation, Genetic
chemical_list: D004271:DNA, Fungal; D000135:Acid Phosphatase