Import of the precursor of the chloroplast Rieske iron-sulphur protein by pea chloroplasts.
cDNA clones encoding the precursor of the Rieske FeS protein of the chloroplast thylakoid membrane have been isolated from a pea leaf cDNA library in lambda gt 11, following screening with antibodies to purified pea chloroplast Rieske FeS protein. The longest cDNA insert of 880 bp encodes a polypeptide of 230 amino acid residues, of which 50 residues constitute an N-terminal cleavable presequence and 180 residues make up the mature protein. Transcription and translation of the cDNA in vitro produced a polypeptide of 26 kDa which was efficiently imported by isolated pea chloroplasts and processed to the mature 20 kDa protein. Southern hybridisation to pea genomic DNA indicated the presence of a single gene encoding the Rieske FeS protein in the haploid genome.
Salter
A H
AH
Department of Biochemistry, Arrhenius Laboratory for Natural Sciences, University of Stockholm, Sweden.
Newman
B J
BJ
Napier
J A
JA
Gray
J C
JC
D000595:Amino Acid Sequence; D001483:Base Sequence; D001692:Biological Transport; D002474:Cell-Free System; D002736:Chloroplasts / Q000378:metabolism*; D003001:Cloning, Molecular; D014450:Electron Transport Complex III; D007887:Fabaceae / Q000235:genetics / Q000378:metabolism*; D015723:Gene Library; D007506:Iron-Sulfur Proteins / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D010946:Plants, Medicinal; D014176:Protein Biosynthesis; D011498:Protein Precursors / Q000235:genetics / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D013347:Subcellular Fractions / Q000378:metabolism; D014158:Transcription, Genetic
D007506:Iron-Sulfur Proteins; D011498:Protein Precursors; C037989:Rieske iron-sulfur protein; D014450:Electron Transport Complex III