[An electromyographic method for determining the antihypoxic activity of preparations].
To measure drug antihypoxic activity, an electromyographic method was worked out. The main idea of the method is to estimate the influence of these substances on the amplitude of slow electric waves of smooth muscles on an isolated strip of rat small intestine in situ. This parameter whose value directly depends on tissue pO2 was recorded under the conditions of artificial ischemia of the intestinal strip. Circulatory hypoxia was simulated by the clamping of mesentery vessels, and the time was determined, during which the amplitude of slow electric waves reduced to 1/3 of the initial value in control animals and rats treated beforehand with the drugs under study. Antihypoxic activity of the drugs was calculated as difference in these time intervals between experiment and control, given in per cent.
Tsibulevskiĭ
A Iu
AIu
Ettinger
A P
AP
Polivoda
M D
MD
Anurov
M V
MV
D000818:Animals; D001562:Benzimidazoles / Q000494:pharmacology / Q000627:therapeutic use; D004353:Drug Evaluation, Preclinical / Q000379:methods; D004576:Electromyography / Q000379:methods*; D004594:Electrophysiology; D005215:Fasting; D006164:Guanylthiourea / Q000494:pharmacology / Q000627:therapeutic use; D006885:Hydroxybutyrates / Q000494:pharmacology / Q000627:therapeutic use; D000860:Hypoxia / Q000188:drug therapy*; D007421:Intestine, Small / Q000187:drug effects / Q000502:physiology; D008094:Lithium / Q000494:pharmacology / Q000627:therapeutic use; D008297:Male; D009130:Muscle, Smooth / Q000187:drug effects / Q000502:physiology; D009942:Organometallic Compounds / Q000494:pharmacology / Q000627:therapeutic use; D051381:Rats
D001562:Benzimidazoles; D006885:Hydroxybutyrates; D009942:Organometallic Compounds; C022826:lithium hydroxybutyrate; C030698:2-mercaptobenzimidazole; D008094:Lithium; D006164:Guanylthiourea
Elektromiograficheskiĭ metod opredeleniia protivogipoksicheskoĭ aktivnosti preparatov.