Protective effect of a prostaglandin oligomer on liver mitochondria in situ: time-shared measurements of fluorescence and reflectance in the cold-preserved rat liver.
The protective effect of a new oligomeric derivative of prostaglandin B2, known as OC-5186, was evaluated using time-sharing spectrofluorometry in the cold-preserved rat liver. Experiments were divided into three groups: in group A, a 5000 ng dose of OC-5186 was administered via the peripheral vein, 1000 ng via the portal vein, and 200 ng/ml in University of Wisconsin (UW) solution; in group B, the OC-5186 dosage was ten times greater than that in group A; in group C (control group), liver procurement and storage were performed without OC-5186. At 0, 12, and 24 h after cold preservation at 4 degrees C, the liver was perfused for 30 min at 12 degrees C with oxygenized Krebs-Henseleit solution, after which the perfusate was switched to deoxygenized Krebs-Henseleit solution. Time sharing spectrofluorometry was used to follow NADH fluorescence at 450 nm with a 360-nm excitation wavelength, as well as the reflectance of cytochrome aa3 with 605 minus 620 nm from oxidation to reduction. Rate constants of NADH fluorescence and cytochrome aa3 reflectance were used as indices of integrity of the mitochondrial respiratory chain. In group C, the rate constant of NADH fluorescence decreased significantly (P < 0.05) from the control value of 8.31 +/- 0.21 x 10(-3) (sec-1) to 4.97 +/- 0.15 x 10(-3) and 5.58 +/- 0.16 x 10(-3) (mean +/- SEM) at 12 and 24 h after cold preservation, respectively.(ABSTRACT TRUNCATED AT 250 WORDS)
Tokuka
A
A
Second Department of Surgery, Faculty of Medicine, Kyoto University, Japan.
Tanaka
A
A
Fujita
T
T
Hayashi
M
M
Kitai
T
T
Yamaoka
Y
Y
Ozawa
K
K
Ohnishi
S T
ST
D000818:Animals; D003080:Cold Temperature; D005453:Fluorescence; D016031:Liver Transplantation; D008297:Male; D008930:Mitochondria, Liver / Q000187:drug effects*; D009243:NAD / Q000378:metabolism; D009926:Organ Preservation; D011456:Prostaglandins B / Q000494:pharmacology*; D051381:Rats; D011917:Rats, Inbred Lew
D011456:Prostaglandins B; D009243:NAD; C070097:OC 5186