title: Enzyme immunoassay for specific analysis of pancreatic stone proteins in human pancreatic juice.
abstract: In order to study the concentration of pancreatic stone protein (PSP) in human pancreatic juice, we investigated the influence of the insoluble form of PSP-S1 converted from PSP-S2-5 on PSP determination and the assay method for PSP-S1 precipitate after solubilizing PSP-S1. When bovine trypsin was added to pancreatic juice, PSP-S1 was converted from PSP-S2-5 and precipitated about 45-85% after 1 h. The precipitated PSP-S1 was dissolved in 0.1 M sodium acetate buffer, pH 4.0, and the concentration was measured by the enzyme immunoassay, with similar reactivity to PSP-S1 and PSP-S2-5. The proposed method can offer accurate and specific analysis of the PSP level in pancreatic juice. The results of the fractionation of pancreatic juice and duodenal juice on Mono S cation-exchange chromatography suggested that the major component of PSP was PSP-S2-5 in pancreatic juice and PSP-S1 in duodenal juice.
authors.affiliation: Department of Clinical Chemistry, Maruko Pharmaceutical Co., Ltd., Kasugai, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Katsuzaki
authors.forename: N
authors.initials: N
authors.lastname: Tatemichi
authors.forename: C
authors.initials: C
authors.lastname: Takeichi
authors.forename: S
authors.initials: S
authors.lastname: Hayakawa
authors.forename: T
authors.initials: T
authors.lastname: Hayakawa
authors.forename: T
authors.initials: T
authors.lastname: Shibata
authors.forename: H
authors.initials: H
authors.lastname: Harada
authors.forename: K
authors.initials: K
authors.lastname: Ochi
mesh_terms: D002135:Calcium-Binding Proteins / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D002908:Chronic Disease; D004386:Duodenum / Q000737:chemistry; D005069:Evaluation Studies as Topic; D006801:Humans; D007124:Immunoenzyme Techniques; D007419:Intestinal Secretions / Q000737:chemistry; D051847:Lithostathine; D009419:Nerve Tissue Proteins; D010189:Pancreatic Juice / Q000737:chemistry*; D010195:Pancreatitis / Q000175:diagnosis / Q000378:metabolism; D012016:Reference Values; D012995:Solubility; D014357:Trypsin
chemical_list: D002135:Calcium-Binding Proteins; D051847:Lithostathine; D009419:Nerve Tissue Proteins; C497617:REG1A protein, human; D014357:Trypsin