Tumor necrosis factor and interleukin-1 induce activin A gene expression in a human bone marrow stromal cell line.
Activin A, a homodimer of the beta A chain, regulates hematopoiesis. In a human bone marrow-derived stromal cell line, KM-102, phorbol myristate acetate, tumor necrosis factor-alpha and interleukin-1 beta induced great increases in beta A chain mRNA levels and production of activin A activities. The phorbol ester-induced beta A chain gene expression was inhibited by cycloheximide and down regulation of protein kinase C, whereas the cytokine-induced expression was little affected by these treatments. These results indicate that the inflammatory cytokines directly stimulate beta A chain gene expression via protein kinase C-independent pathways.
Takahashi
S
S
Fourth Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Japan.
Uchimaru
K
K
Harigaya
K
K
Asano
S
S
Yamashita
T
T
D028341:Activins; D015152:Blotting, Northern; D001853:Bone Marrow / Q000502:physiology*; D001854:Bone Marrow Cells; D002460:Cell Line; D017077:Culture Media, Conditioned; D003513:Cycloheximide / Q000494:pharmacology; D004247:DNA / Q000032:analysis / Q000235:genetics; D015870:Gene Expression / Q000187:drug effects; D006133:Growth Substances / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D007265:Inhibins / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification; D007375:Interleukin-1 / Q000494:pharmacology*; D046911:Macromolecular Substances; D015240:Phorbol 12,13-Dibutyrate / Q000494:pharmacology; D011493:Protein Kinase C / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D011994:Recombinant Proteins / Q000494:pharmacology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
D017077:Culture Media, Conditioned; D006133:Growth Substances; D007375:Interleukin-1; D046911:Macromolecular Substances; D012333:RNA, Messenger; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; D028341:Activins; D015240:Phorbol 12,13-Dibutyrate; D007265:Inhibins; D004247:DNA; D003513:Cycloheximide; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate