Extracellular diaphorase-like activity as a marker of cytolytic damage to cells.
A new variant of the enzyme release colorimetric method for NK/LAK cytotoxicity is described. Kinetic determination of diaphorase released from damaged target cells (TC) provided an exact measurement of the lytic activity of effector cells (EC). A polyenzyme testing system permitted the use of a wide spectrum of TC. The percentage cytotoxicity and the kinetics of the process coincide, within experimental error, with data from staining with Trypan blue. The method is simple and convenient and can be applied to study cell-mediated cytotoxicity (CMC).
Petrov
R V
RV
Pushchino Branch, Shemyakin Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow Region.
Kalinina
A R
AR
Sadovnikov
V B
VB
Vodyanik
M A
MA
D015415:Biomarkers; D003602:Cytotoxicity, Immunologic; D008058:Dihydrolipoamide Dehydrogenase / Q000378:metabolism*; D005110:Extracellular Space / Q000201:enzymology* / Q000276:immunology*; D006801:Humans; D015979:Killer Cells, Lymphokine-Activated / Q000276:immunology; D007694:Killer Cells, Natural / Q000276:immunology; D007700:Kinetics; D014407:Tumor Cells, Cultured / Q000276:immunology
D015415:Biomarkers; D008058:Dihydrolipoamide Dehydrogenase