Leukotriene formation by mouse connective tissue mast cells.
Stimulation of peritoneal cells from BALB/c, CBA/J or WBB6F1(-)+/+ mice with IgE/antigen caused the release of mast cell granules and leukotriene C4. No leukotriene formation was observed with peritoneal cells from mast cell-deficient WBB6F1-W/Wv mice. Mast cells (greater than 98% purity), separated on metrizamide gradients, did not synthesize detectable amounts of leukotriene C4 when challenged immediately after purification. Co-culture of the mast cells with 3T3 fibroblasts restored the capability of the mast cells to produce leukotrienes. Addition of IL-3 during culture enhanced the synthesis of this eicosanoid. The 5-lipoxygenase inhibitor A-63162 blocked the leukotriene formation. Western blot analysis confirmed the presence of 5-lipoxygenase in connective tissue mast cells. These experiments demonstrate that mouse peritoneal (connective tissue) mast cells can produce significant amounts of leukotrienes.
Jakschik
B A
BA
Department of Molecular Biology and Pharmacology, Washington University School of Medicine, St. Louis, MO 63110.
Harrington
L F
LF
Malaviya
R
R
D000081:Acetamides / Q000494:pharmacology; D000818:Animals; D003238:Connective Tissue / Q000378:metabolism*; D003239:Connective Tissue Cells; D016859:Lipoxygenase Inhibitors / Q000494:pharmacology; D008407:Mast Cells / Q000378:metabolism*; D051379:Mice; D008815:Mice, Inbred Strains; D010647:Phenyl Ethers; D013189:SRS-A / Q000096:biosynthesis*
D000081:Acetamides; D016859:Lipoxygenase Inhibitors; D010647:Phenyl Ethers; D013189:SRS-A; C068663:N-hydroxy-N-(1-(4-(phenylmethoxy)phenyl)ethyl)-acetamide