Androgen metabolism assessment by routine gas chromatography/mass spectrometry profiling of plasma steroids: Part 1, Unconjugated steroids.
Using gas chromatography/mass spectrometry we have developed a method for the simultaneous determination of six plasma steroids: testosterone, 4-androstenedione, 17 alpha-hydroxyprogesterone, 5 alpha-androstane-3 alpha,17 beta-diol, 5 alpha-dihydrotestosterone, and dehydroepiandrosterone. For each analyte, a deuterium-labeled internal standard was used for quantification. Due to the high isotopic purity of our standards, no complex corrections for isotope contributions were necessary. The procedure provides a sensitive and specific technique with good accuracy and precision.
Wudy
S A
SA
First Department of Pediatrics, University of Ulm, Ulm/Donau, Germany.
Wachter
U A
UA
Homoki
J
J
Teller
W M
WM
Shackleton
C H
CH
D019326:17-alpha-Hydroxyprogesterone; D000728:Androgens / Q000097:blood*; D015113:Androstane-3,17-diol / Q000097:blood; D000735:Androstenedione / Q000097:blood; D002648:Child; D003687:Dehydroepiandrosterone / Q000097:blood; D003903:Deuterium; D013196:Dihydrotestosterone / Q000097:blood; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D006908:Hydroxyprogesterones / Q000097:blood; D008297:Male; D013739:Testosterone / Q000097:blood
D000728:Androgens; D006908:Hydroxyprogesterones; D013196:Dihydrotestosterone; D015113:Androstane-3,17-diol; D013739:Testosterone; D000735:Androstenedione; D003687:Dehydroepiandrosterone; D019326:17-alpha-Hydroxyprogesterone; D003903:Deuterium