T cell receptor usage by HLA-DQw8-specific T cell clones.
To investigate whether T cells recognizing the same HLA molecule may demonstrate homology in parts of their TCR, five different HLA-DQw8-specific T lymphocyte clones (TLC) were studied. The TCR alpha and -beta genes of four alloreactive, HLA-DQw8-specific and one antigen-specific TLC were sequenced. All TLC used different V alpha and V beta genes. However, four of the TLC shared a certain CDR1 beta motif and all five used either J beta 2.3 or -2.5. In addition, two used the same J alpha. The results indicate a possible preferential usage of certain TCR structures by T cells specific for DQw8.
Hansen
T
T
Institute of Transplantation Immunology, National Hospital, Oslo, Norway.
Lundin
K E
KE
Markussen
G
G
Thorsby
E
E
D000595:Amino Acid Sequence; D001483:Base Sequence; D002999:Clone Cells; D015870:Gene Expression; D005796:Genes; D006683:HLA-DQ Antigens / Q000276:immunology*; D006801:Humans; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics*; D016415:Sequence Alignment; D013601:T-Lymphocytes / Q000276:immunology*
D006683:HLA-DQ Antigens; C109756:HLA-DQ8 antigen; D009838:Oligodeoxyribonucleotides; D016693:Receptors, Antigen, T-Cell, alpha-beta