Expression of ileal glucagon and peptide tyrosine-tyrosine genes. Response to inhibition of polyamine synthesis in the presence of massive small-bowel resection.
Massive small-bowel resection results in a marked adaptive response in the residual terminal ileum. Increased polyamine synthesis is a necessary component of this response. The ileal L-cell-derived peptides enteroglucagon and peptide tyrosine tyrosine (PYY) have been implicated as humoral mediators of this response. We have previously reported a rapid and sustained increase in glucagon mRNA concentrations after massive small-bowel resection. In this study using an inhibitor of the rate-limiting enzyme in polyamine biosynthesis, ornithine decarboxylase, we have demonstrated that the response of the glucagon and PYY genes to massive small-bowel resection is dependent on polyamine biosynthesis. In addition, we have examined the response of both the ornithine decarboxylase and c-jun genes in this model of intestinal adaptation.
Taylor
R G
RG
Department of Surgery, Royal Children's Hospital, Parkville, Australia.
Beveridge
D J
DJ
Fuller
P J
PJ
D000818:Animals; D015152:Blotting, Northern; D004151:Dipeptides / Q000235:genetics*; D000518:Eflornithine / Q000494:pharmacology; D005260:Female; D015870:Gene Expression; D004763:Glucagon-Like Peptides / Q000235:genetics*; D007082:Ileum / Q000378:metabolism* / Q000601:surgery; D009693:Nucleic Acid Hybridization; D009955:Ornithine Decarboxylase / Q000235:genetics; D011073:Polyamines / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D014404:Tubulin / Q000235:genetics
D004151:Dipeptides; D011073:Polyamines; D012333:RNA, Messenger; D014404:Tubulin; C042696:tyrosyltyrosine; D004763:Glucagon-Like Peptides; D009955:Ornithine Decarboxylase; D000518:Eflornithine