Immunogenicity and protective efficacy of solubilized merozoite-enriched Theileria sergenti immunogens. II: Protection against natural exposure under field conditions.
A Theileria sergenti soluble merozoite preparation containing the 29, 34, 35 and 105 KD as the immunodominant polypeptides, was evaluated for efficacy, safety and protectivity in Holstein calves against virulent field tick challenge. The soluble antigens (100 mg/dose) were fortified with either complete or incomplete Freund's adjuvant. Twenty naive calves, aged one month, were subcutaneously inoculated with the preparation and a booster dose was administered 4 weeks later. Twenty additional calves served as controls. Five weeks after the booster dose, vaccinates and uninoculated controls were moved to a pasture, a heavily tick infested area in Cheju-do, Korea. The vaccinates showed negligible change in hematocrit and total RBC count whereas control animals showed significant (p < 0.05) hematological changes and associated anemia. Only 30% of vaccinates required chemotherapy after the experiment was terminated. All control animals required chemotherapy and 25% received blood transfusion. The highest percent parasitized erythrocytes in vaccinated cattle was 0.4% as compared with 3.6% among controls during the month of July. A significant difference (p < 0.05) was observed in the rate of body weight increase. Significant differences were also noted in serum albumin, aspartate aminotransferase, total protein and bilirubin. Significantly more vaccinated cattle maintained normal ranges of hematological and biochemical values as compared with the control group. It is suggested that soluble merozoite T. sergenti antigens may be potential vaccine candidates for developing a genetic vaccine in Korea.
Baek
B K
BK
College of Veterinary Medicine, Chonbuk National University, Chonju, Korea.
Kim
B S
BS
Choi
I H
IH
Yang
K C
KC
Hansen
R
R
Kakoma
I
I
D000818:Animals; D000953:Antigens, Protozoan; D000954:Antigens, Surface / Q000276:immunology*; D001835:Body Weight; D002417:Cattle; D004906:Erythrocyte Count; D006400:Hematocrit; D015800:Protozoan Proteins / Q000276:immunology*; D016052:Protozoan Vaccines; D012995:Solubility; D016794:Theileria / Q000276:immunology*; D013801:Theileriasis / Q000097:blood / Q000517:prevention & control*
D000953:Antigens, Protozoan; D000954:Antigens, Surface; D015800:Protozoan Proteins; D016052:Protozoan Vaccines; C065974:merozoite surface protein 2, Plasmodium