Decortication and striatal mRNA: increases of mRNA for fibronectin, but not of NCAM or alpha-1 tubulin.
Ipsilateral frontal cortex lesions damaged the corticostriatal input. Northern hybridization analysis showed increases in fibronectin (FN) mRNA, but not changes in mRNA for neural cell adhesion molecule (NCAM), neuro-filament-68 (NF-68) or alpha-tubulin (alpha-1T) 72 h post-lesion. In situ hybridization resolved a different spatial-temporal distribution. The superficial cell layer beneath the wound cavity showed transient elevations of FN mRNA that peaked at 72 h post-lesion. However, in the ipsilateral striatum, FN mRNA was maximal at later times than in the wound cavity, at 240 h post-lesion. Changes in NCAM and alpha-tubulin mRNAs in response to decortication occur only around the wound cavity but not in the deafferentated striatum. The different time courses of mRNA revealed by Northern blot analysis and in situ hybridization are most probably due to contamination of the ipsilateral striatum at dissection with superficial tissue adjacent to the wound cavity. These results suggest that cellular responses to ipsilateral decortication consist of two phases: (i) a wound healing process; and (ii) striatal responses to deafferentation.
Popa-Wagner
A
A
Ethel Percy Andrus Gerontology Center, Department of Biological Sciences, University of Southern California, Los Angeles.
Day
J R
JR
Platt
D
D
Pasinetti
G M
GM
D000818:Animals; D015152:Blotting, Northern; D015816:Cell Adhesion Molecules, Neuronal / Q000096:biosynthesis*; D003342:Corpus Striatum / Q000378:metabolism*; D003655:Decerebrate State / Q000378:metabolism*; D005353:Fibronectins / Q000096:biosynthesis*; D008297:Male; D009416:Nerve Regeneration; D016900:Neurofilament Proteins / Q000096:biosynthesis; D015347:RNA Probes; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D011916:Rats, Inbred F344; D012584:Sciatic Nerve / Q000502:physiology; D014404:Tubulin / Q000096:biosynthesis*
D015816:Cell Adhesion Molecules, Neuronal; D005353:Fibronectins; D016900:Neurofilament Proteins; D015347:RNA Probes; D012333:RNA, Messenger; D014404:Tubulin