title: HLA-DP restricted Chlamydia trachomatis specific synovial fluid T cell clones in Chlamydia induced Reiter's disease.
abstract: Synovial fluid (SF) mononuclear cells from a patient with Chlamydia trachomatis induced acute Reiter's disease were directly by limiting dilution in a representative protocol using phytohemagglutinin in the cloning medium. Out of 76 alpha beta-TCR+ CD4+ T lymphocyte clones, 7 were shown to specifically recognize C. trachomatis in a proliferation assay. The antigen recognition of these clones was HLA-DP restricted. Unexpectedly, 2 HLA-DR restricted clones showed a proliferative response to Yersinia enterocolitica O3, though the patient had no history of yersinia infection. The high frequency of SF derived T cells with specificity for species-specific chlamydial antigens and the limited diversity of HLA class II restriction of these clones may indicate an oligoclonal synovial T cell response to persistent intraarticular chlamydia.
authors.affiliation: Department of Internal Medicine, Johannes Gutenberg-University, Mainz, Germany.
authors.forename: E
authors.initials: E
authors.lastname: Hermann
authors.forename: W J
authors.initials: WJ
authors.lastname: Mayet
authors.forename: H
authors.initials: H
authors.lastname: Thomssen
authors.forename: J
authors.initials: J
authors.lastname: Sieper
authors.forename: T
authors.initials: T
authors.lastname: Poralla
authors.forename: K H
authors.initials: KH
authors.lastname: Meyer zum Büschenfelde
authors.forename: B
authors.initials: B
authors.lastname: Fleischer
mesh_terms: D000328:Adult; D000942:Antigens, Bacterial / Q000032:analysis; D001170:Arthritis, Infectious / Q000276:immunology / Q000473:pathology; D016918:Arthritis, Reactive / Q000209:etiology / Q000276:immunology* / Q000473:pathology; D015704:CD4 Antigens / Q000032:analysis / Q000276:immunology; D002358:Cartilage, Articular / Q000276:immunology / Q000473:pathology; D002690:Chlamydia Infections / Q000276:immunology; D002692:Chlamydia trachomatis / Q000276:immunology* / Q000502:physiology*; D002999:Clone Cells; D006682:HLA-DP Antigens / Q000276:immunology*; D006684:HLA-DR Antigens / Q000276:immunology; D006801:Humans; D008297:Male; D010835:Phytohemagglutinins; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000032:analysis / Q000276:immunology; D013045:Species Specificity; D013582:Synovial Fluid / Q000166:cytology*; D013601:T-Lymphocytes / Q000276:immunology* / Q000473:pathology; D015009:Yersinia Infections / Q000276:immunology / Q000473:pathology; D015008:Yersinia enterocolitica / Q000276:immunology / Q000502:physiology
chemical_list: D000942:Antigens, Bacterial; D015704:CD4 Antigens; D006682:HLA-DP Antigens; D006684:HLA-DR Antigens; D010835:Phytohemagglutinins; D016693:Receptors, Antigen, T-Cell, alpha-beta