Cytological and ultrastructural investigation on osteoblastic and preosteoclastic cells grown in vitro in the presence of ipriflavone: preliminary results.
The effects of ipriflavone on bone cells were studied in vitro on pre-osteoclastic (FLG 29.1) and osteoblast-like (Saos-2) cells grown for 48 h either separately or in co-cultures, with or without the addition of PTH. Histological, ultrastructural and histochemical (TRAP-activity demonstration) methods were used. The main results show that ipriflavone reduces replication of FLG 29.1 cells and inhibits TRAP production by these cells both in controls and in co-cultures treated with PTH. Moreover, it has a moderate stimulatory effect on proliferation of osteoblast-like cells and reduces the PTH-induced degenerative changes of Saos-2 cells. These results suggest that the inhibitory effect of ipriflavone on FLG 29.1 cells might be indirect and might be mediated by the osteoblast-like cells.
Bonucci
E
E
Department of Human Biopathology, University La Sapienza, Rome, Italy.
Silvestrini
G
G
Ballanti
P
P
Masi
L
L
Franchi
A
A
Bufalino
L
L
Brandi
M L
ML
D000135:Acid Phosphatase / Q000378:metabolism; D002448:Cell Adhesion / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D000091344:Filaggrin Proteins; D006801:Humans; D007529:Isoflavones / Q000494:pharmacology*; D008854:Microscopy, Electron; D010006:Osteoblasts / Q000187:drug effects* / Q000648:ultrastructure; D010010:Osteoclasts / Q000187:drug effects* / Q000648:ultrastructure; D010281:Parathyroid Hormone / Q000494:pharmacology; D014407:Tumor Cells, Cultured
C000717249:FLG protein, human; D000091344:Filaggrin Proteins; D007529:Isoflavones; D010281:Parathyroid Hormone; C018986:ipriflavone; D000135:Acid Phosphatase