Collection of blood mononuclear cells by leukapheresis for transplantation in a Yucatan miniature swine model.
A large animal model is needed to evaluate new apheresis technologies. These technologies include novel methods of harvesting the blood mononuclear cell population which contains the hematopoietic stem cells needed to restore hematopoiesis in recipients of hematopoietically lethal therapy and the use of cytokines to provide a safe and predictable method of manipulating these circulating hematopoietic stem cells. We describe the methods used to collect mononuclear cells by leukapheresis from Yucatan miniature swine. These animals are of sufficient size to tolerate the procedures and have many physiologic and hematologic similarities to man. They are of good temperament and are easily trained. Long-term venous access was obtained using single lumen silicone rubber catheters placed in the inferior vena cava. The animals were apheresed while fully awake using a Haemonetics Model V50 machine and a modified lymphocyte collection protocol. The procedure was highly efficient for the collection of mononuclear cells and a 10 pass procedure yielded a product which contained 19.7 x 10(9) mononuclear cells, 10.7 x 10(9) granulocytes, and 17 ml of erythrocytes in a volume of approximately 100 ml. This product can be cryopreserved and used for subsequent transplantation. The content of four apheresis procedures provides hematopoietic reconstitution of lethally irradiated swine on a time scale equivalent to transplantation of optimal numbers of bone marrow cells.
Smith
D M
DM
Department of Pathology, University of Nebraska Medical Center, Omaha.
Stribley
J A
JA
Lieberman
R P
RP
Sharp
J G
JG
D000818:Animals; D001854:Bone Marrow Cells; D002405:Catheterization, Central Venous; D007937:Leukapheresis; D007963:Leukocytes, Mononuclear / Q000637:transplantation*; D008954:Models, Biological; D008991:Monitoring, Physiologic; D013048:Specimen Handling / Q000379:methods; D013552:Swine; D013556:Swine, Miniature; D014682:Vena Cava, Inferior; D014916:Whole-Body Irradiation