Extracellular perfusion controller for electrophysiology.
This paper describes the design and utility of a perfusion controller to facilitate the handling of multiple extracellular solutions. The controller consists of an interface box with both manual and computer controls and a set of solenoid valves. This provides remote handling of the switching of multiple solutions into an experimental chamber. It is described how this system can be used in whole-cell patch-clamp experiments to measure various ion channel currents as well as Na/K pump currents. Other possible uses are discussed along with suggestions for interfacing the perfusion controller with existing perfusion systems.
Stimers
J R
JR
Department of Pharmacology and Toxicology, University of Arkansas for Medical Sciences, Little Rock 72205.
D000818:Animals; D003201:Computers; D004594:Electrophysiology / Q000295:instrumentation*; D006321:Heart / Q000187:drug effects; D009206:Myocardium / Q000166:cytology; D010042:Ouabain / Q000494:pharmacology; D010477:Perfusion / Q000295:instrumentation*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012984:Software
D010042:Ouabain