title: Mid-infrared pulsed laser ablation of the arterial wall. Mechanical origin of "acoustic" wall damage and its effect on wall healing.
abstract: Pulsed mid-infrared lasers are an alternative to excimer lasers for transluminal angioplasty. The mid-infrared lasers, however, were reported to produce "acoustic" wall damage that might impair the immediate and long-term results. To study the immediate and long-term effects on the arterial wall, 184 craters (1 mm diameter and 1 mm depth) were produced perpendicular to the intimal lining in the thoracic aortas of pigs. Three types of craters were evaluated: Ho-YSGG laser-induced (lambda = 2.09 microns, 2 pulses of 500 microseconds, 0.50 joule/pulse, 50 gm force), mechanically drilled, and "acoustic" craters. "Acoustic" craters were produced by two laser pulses delivered into a saline-filled metal fiber cap, which was placed in a mechanically drilled crater. The metal cap was provided with four outlets for water vapor and isolated "acoustic" from optical and thermal laser effects. The pigs survived 3, 14, and 28 days. Arterial wall damage, medial necrosis, and wall healing were assessed microscopically. At 3 days, laser and "acoustic" craters were accompanied by large tissue ruptures (2.7 +/- 0.9 mm and 2.9 +/- 0.8 mm, respectively, mean +/- standard deviation). These were attributed to large vapor bubbles expanding within the tissue. A zone of medial necrosis was observed adjacent to the laser craters (0.43 +/- 0.15 mm) and to the "acoustic" craters (0.17 +/- 0.14 mm). Neither ruptures nor necrosis was observed with the mechanical craters. At 2 and 4 weeks, the necrotic areas were repopulated with smooth muscle cells and all craters were adequately filled with smooth muscle cells, without any sign of an exaggerated proliferative response. We conclude that within the arterial wall, Ho-YSGG ablation was accompanied by the rapid expansion of a water vapor bubble. The formation of the relatively large vapor bubble is inherent to the use of a mid-infrared laser. The risk of creating dissections clinically, when delivering Ho-YSGG laser pulses, remains to be determined. The present study provided no indication that the arterial wall fissures might affect the restenosis rate unfavorably by promoting myointimal proliferation.
authors.affiliation: Department of Cardiology, University Hospital Utrecht, The Netherlands.
authors.forename: L
authors.initials: L
authors.lastname: van Erven
authors.forename: T G
authors.initials: TG
authors.lastname: van Leeuwen
authors.forename: M J
authors.initials: MJ
authors.lastname: Post
authors.forename: M J
authors.initials: MJ
authors.lastname: van der Veen
authors.forename: E
authors.initials: E
authors.lastname: Velema
authors.forename: C
authors.initials: C
authors.lastname: Borst
mesh_terms: D016342:Angioplasty, Laser; D000818:Animals; D001013:Aorta, Thoracic / Q000473:pathology* / Q000601:surgery*; D005260:Female; D007259:Infrared Rays; D013552:Swine; D014945:Wound Healing