Muscle performance and adenine-nucleotides status in MCA-sarcoma tumor-bearing rats.
The reduction in DNA, RNA, amino acid, and total protein in muscle tissue of tumor-bearing rats may influence muscle function. The effects of MCA-sarcoma tumor burden on muscle performance and adenine nucleotides was evaluated in three fiber types of skeletal muscle. Twenty-one days after MCA-sarcoma tumor inoculation, the gastrocnemius-soleus muscle group of Fischer 344 rats was stimulated using an in situ preparation; tetanic stimulation for 10 min at 7.5, 15, or 30 tetani per min (TPM) or 60 TPM for 5 min (n = 6 control and six tumor-bearing rats/group). ATP, ADP, AMP, IMP, phosphocreatine, and creatine content in white and red gastrocnemius muscle and soleus muscle was measured. There were no differences among controls and tumor-bearing rats in force output; however, ATP content in the soleus muscle of tumor-bearing rats was significantly reduced after 30 TPM for 10 min or 60 TPM for 5 min. The performance of skeletal muscle, over a wide range of stimulation frequency, in tumor-bearing rats does not appear to be influenced by changes in adenine nucleotide content.
Muscaritoli
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Department of Surgery, University Hospital, SUNY Health Science Center, Syracuse 13210.
Whitlock
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Meguid
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MM
D000227:Adenine Nucleotides / Q000502:physiology*; D000818:Animals; D002455:Cell Division / Q000502:physiology; D004558:Electric Stimulation; D007537:Isometric Contraction / Q000502:physiology*; D008297:Male; D009132:Muscles / Q000503:physiopathology*; D009368:Neoplasm Transplantation; D051381:Rats; D011916:Rats, Inbred F344; D012513:Sarcoma, Experimental / Q000503:physiopathology*
D000227:Adenine Nucleotides