Transfer of mobilizable plasmids to Sorangium cellulosum and evidence for their integration into the chromosome.
Recombinant vectors derived from the broad-host-range mobilizable plasmid pSUP2021 were constructed and transferred by IncP-mediated conjugation from Escherichia coli to Sorangium cellulosum, where they were integrated into the chromosome by homologous recombination and maintained stably. This appears to be the first system of gene transfer to S. cellulosum.
Jaoua
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S
Department of Biotechnology, Ciba Geigy, Ltd., Basel, Switzerland.
Neff
S
S
Schupp
T
T
D002876:Chromosomes, Bacterial; D003227:Conjugation, Genetic; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D005822:Genetic Vectors; D009231:Myxococcales / Q000235:genetics*; D010957:Plasmids; D011995:Recombination, Genetic
D004269:DNA, Bacterial