title: The clonogen content of murine intestinal crypts: dependence on radiation dose used in its determination.
abstract: The number of colony-forming (clonogenic) cells in each of the crypts in mouse small intestine was deduced using a two-dose irradiation technique. The number was 7.5 +/- 0.8 cells using two equal doses each less than 9 Gy and 38 +/- 7 cells using 9 Gy or more per dose. The significant dose dependence could not be accounted for by considerations of intra- or intercrypt variability, or by the factor introduced to correct the sampling frequency for the influence of crypt size. The results suggest that more colony-forming cells may be recruited when the injury is more severe.
authors.affiliation: Cancer Research Campaign Department of Experimental Radiation Oncology, Paterson Institute for Cancer Research, Christie Hospital (NHS) Trust, Manchester, United Kingdom.
authors.forename: J H
authors.initials: JH
authors.lastname: Hendry
authors.forename: S A
authors.initials: SA
authors.lastname: Roberts
authors.forename: C S
authors.initials: CS
authors.lastname: Potten
mesh_terms: D000818:Animals; D003114:Colony-Forming Units Assay / Q000379:methods*; D004307:Dose-Response Relationship, Radiation; D007421:Intestine, Small / Q000166:cytology / Q000528:radiation effects*; D008297:Male; D051379:Mice; D013234:Stem Cells / Q000528:radiation effects*