title: Detection of Mycobacterium paratuberculosis antigen with colloidal immunogold in naturally infected sheep.
abstract: The anti-Mycobacterium paratuberculosis polyclonal serum is proved useful for labelling Mycobacterium paratuberculosis in glutaraldehyde-osmium-fixed and epon-embedded intestinal samples from sheep with clinical symptoms of paratuberculosis. M. paratuberculosis marked with antibody-coated colloidal gold stain was seen in macrophages, epithelioid cells, giant cells and neutrophils throughout intestinal mucosa. In large macrophages with a low lysosomal content, a great number of intact mycobacteria was seen within phagosomes. In macrophages with average lysosomal content, very few intact mycobacteria or mycobacterial debris were present and lysosome-phagosome fusions were observed. Mycobacteria within neutrophils were scanty. These results show the usefulness of colloidal immunogold techniques for studies of the pathogenesis of paratuberculosis.
authors.affiliation: Unidad Docente de Histología y Anatomía Patológica, Facultad de Veterinaria, Universidad de Murcia, España.
authors.forename: J A
authors.initials: JA
authors.lastname: Navarro
authors.forename: J
authors.initials: J
authors.lastname: Sanchez
authors.forename: A
authors.initials: A
authors.lastname: Bernabe
authors.forename: M A
authors.initials: MA
authors.lastname: Gomez
authors.forename: S
authors.initials: S
authors.lastname: Gomez
authors.forename: J
authors.initials: J
authors.lastname: Seva
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000032:analysis*; D007150:Immunohistochemistry; D016253:Microscopy, Immunoelectron; D016927:Mycobacterium avium subsp. paratuberculosis / Q000276:immunology*; D010283:Paratuberculosis / Q000382:microbiology*; D010586:Phagocytes / Q000382:microbiology*; D012756:Sheep; D012757:Sheep Diseases / Q000382:microbiology*
chemical_list: D000942:Antigens, Bacterial