Chiral separation of barbiturates and hydantoins by reversed-phase high-performance liquid chromatography using a 25 or 50 mm short ODS cartridge column via beta-cyclodextrin inclusion complexes.
A high-performance liquid chromatographic method on a 25 or 50 mm short ODS cartridge column has been developed for the resolution of the enantiomers of some optically active barbiturates and hydantoins in human serum. beta-Cyclodextrin was used in the mobile phase. This method also seems to be an easy and effective way to test whether beta-cyclodextrin would be a useful chiral discriminator for a particular racemate.
Eto
S
S
Department of Hospital Pharmacy, School of Medicine, University of Occupational and Environmental Health, Kitakyushu, Japan.
Noda
H
H
Noda
A
A
D001463:Barbiturates / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003505:Cyclodextrins; D006801:Humans; D006827:Hydantoins / Q000097:blood*; D008618:Mephobarbital / Q000097:blood; D010672:Phenytoin / Q000031:analogs & derivatives / Q000097:blood; D013237:Stereoisomerism; D047392:beta-Cyclodextrins
D001463:Barbiturates; D003505:Cyclodextrins; D006827:Hydantoins; D047392:beta-Cyclodextrins; C027364:5-(3-hydroxyphenyl)-5-phenylhydantoin; D008618:Mephobarbital; D010672:Phenytoin; C031215:betadex