Activation of human prothrombin by the venom of Lonomia achelous (Cramer) caterpillars.
In this paper, we demonstrated that the procoagulant action of Lonomia achelous (Cramer) is due in part to a component that activates prothrombin. The activation by crude venom and Fractions obtained by gel filtration on Sephadex G-75 is not dependent of phospholipid, Ca++ or Factor V. The activation of prothrombin by Fraction I is greatly stimulated by Factor V in the presence of phospholipid and Ca++; in presence of SBTI, we found that the Fraction I and Factor Xa act in a similar manner. These results suggest that the Fraction I is a Factor Xa - like prothrombin activator.
Guerrero
B
B
Laboratorio de Fisiopatología, Instituto Venezolano de Investigaciones Científicas (IVIC), Caracas-Venezuela.
Arocha-Piñango
C L
CL
D000818:Animals; D001180:Arthropod Venoms / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D002118:Calcium / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D005165:Factor V / Q000378:metabolism; D005170:Factor X / Q000378:metabolism; D015951:Factor Xa / Q000378:metabolism*; D006458:Hemolymph / Q000737:chemistry; D006801:Humans; D007915:Lepidoptera / Q000378:metabolism*; D010743:Phospholipids / Q000494:pharmacology; D011516:Prothrombin / Q000378:metabolism*; D013917:Thrombin / Q000096:biosynthesis*; D014361:Trypsin Inhibitors / Q000494:pharmacology
D001180:Arthropod Venoms; D010743:Phospholipids; D014361:Trypsin Inhibitors; C022475:prothrombinase complex; D005165:Factor V; D011516:Prothrombin; D005170:Factor X; D013917:Thrombin; D015951:Factor Xa; D002118:Calcium