title: Elevated muscle acidity and energy production during exhaustive exercise in humans.
abstract: This study examined the effect of previous intense exercise on energy production during exhaustive exercise. Subjects (n = 6) performed dynamic knee extensor exercise to exhaustion twice (Ex1 and Ex2) separated by 16 min of recovery consisting of 10 min of rest, 3.5 min of very high-intensity intermittent exercise, and a further 2.5 min of rest. This resulted in an elevated muscle lactate concentration of 13.1 mmol/kg wet wt before Ex2. Muscle lactate concentration was the same at end of Ex1 and Ex2, but the accumulation of lactate and net lactate release during Ex2 was reduced (P < 0.05) by 67 and 38%, respectively. The time to exhaustion was 3.73 and 2.98 min, respectively, and the mean rate of net lactate production for Ex2 was lower (P < 0.05) than for Ex1 (4.6 +/- 1.2 and 9.6 +/- 1.7 mmol.min-1.kg wet wt-1, respectively). Leg O2 uptake was the same for Ex1 and Ex2. Muscle pH (6.85) was lowered (P < 0.05) before Ex2, but at the end of Ex2 (6.77) it tended (P < 0.1) to be higher compared with that at the end of Ex1 (6.73). In summary, the net lactate production rate is reduced but the aerobic energy production is not significantly altered when intense exercise is repeated. Fatigue and the lowered glycolysis do not appear to be caused by the elevated acidity per se before exercise.
authors.affiliation: August Krogh Institute, Copenhagen, Denmark.
authors.forename: J
authors.initials: J
authors.lastname: Bangsbo
authors.forename: T
authors.initials: T
authors.lastname: Graham
authors.forename: L
authors.initials: L
authors.lastname: Johansen
authors.forename: S
authors.initials: S
authors.lastname: Strange
authors.forename: C
authors.initials: C
authors.lastname: Christensen
authors.forename: B
authors.initials: B
authors.lastname: Saltin
mesh_terms: D000136:Acid-Base Equilibrium; D000143:Acids / Q000378:metabolism*; D000328:Adult; D000332:Aerobiosis; D000693:Anaerobiosis; D001769:Blood / Q000378:metabolism; D050260:Carbohydrate Metabolism; D004734:Energy Metabolism; D006801:Humans; D007474:Ion Exchange; D008297:Male; D009132:Muscles / Q000378:metabolism*; D010807:Physical Endurance; D011188:Potassium / Q000378:metabolism; D013997:Time Factors
chemical_list: D000143:Acids; D011188:Potassium