Disulfide-linked S100 beta dimers and signal transduction.
S100 beta is a calcium-binding protein with neurotrophic and mitogenic activities, both of which may be mediated by the protein's ability to stimulate an increase in intracellular free calcium ([Ca2+]i). These extracellular trophic activities of S100 beta require a disulfide-linked, dimeric form of the protein. In this chapter, we present a minireview on the current state of knowledge concerning extracellular functions of S100 beta, with emphasis on the potential relevance of these activities to neuropathological disorders. We also report a simplified procedure for preparation of pharmacological amounts of biologically active S100 beta dimers, based on the finding that formation of disulfide-linked S100 beta dimers can be stimulated by the presence of calcium or lipid.
Barger
S W
SW
Department of Cell Biology, Vanderbilt University, Nashville, TN 37232-6600.
Wolchok
S R
SR
Van Eldik
L J
LJ
D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002135:Calcium-Binding Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000502:physiology; D002493:Central Nervous System Diseases / Q000378:metabolism; D004220:Disulfides / Q000032:analysis*; D006801:Humans; D008055:Lipids / Q000494:pharmacology; D009414:Nerve Growth Factors / Q000737:chemistry* / Q000302:isolation & purification / Q000502:physiology; D011994:Recombinant Proteins / Q000302:isolation & purification; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000502:physiology; D015398:Signal Transduction
D002135:Calcium-Binding Proteins; D004220:Disulfides; D008055:Lipids; D009414:Nerve Growth Factors; D011994:Recombinant Proteins; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins; C579160:S100B protein, human; D002118:Calcium