title: The complete nucleotide sequence of turnip mosaic potyvirus RNA.
abstract: The complete RNA genome of turnip mosaic potyvirus (TuMV) was amplified by seven consecutive reverse transcriptase-polymerase chain reactions and cloned into pUC9. The viral RNA is 9830 nucleotides long and contains a single open reading frame (ORF) of 9489 bases encoding a large polyprotein of 3863 amino acids with a calculated M(r) of 358,000. The non-coding region (NCR) preceding the ORF is 129 nucleotides long and has a high AU content (70%). Its predicted secondary structure is characterized by a hairpin loop with a free energy loss of -69.9 kJ/mol. The termination codon is followed by an AU-rich NCR of 209 bases, excluding the poly(A) tail. Seven potential nuclear inclusion a proteinase (NIa-Pro) recognition heptapeptides are found in the polyprotein. Their sequences agree with consensus potyviral NIa-Pro cleavage sequences except for that at the 6K-VPg site, which is characterized by a glutamic acid residue preceding the hydrolysed peptide bond. The TuMV proteins are similar to their corresponding potyviral proteins.
authors.affiliation: Centre de Recherche en Virologie, Institut Armand-Frappier, Ville de Laval, Québec, Canada.
authors.forename: O
authors.initials: O
authors.lastname: Nicolas
authors.forename: J F
authors.initials: JF
authors.lastname: Laliberté
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D001937:Brassica / Q000382:microbiology; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D016384:Consensus Sequence; D010450:Endopeptidases / Q000378:metabolism; D016679:Genome, Viral; D008969:Molecular Sequence Data; D009029:Mosaic Viruses / Q000235:genetics*; D009690:Nucleic Acid Conformation; D016366:Open Reading Frames / Q000235:genetics; D011499:Protein Processing, Post-Translational; D012367:RNA, Viral / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D014764:Viral Proteins / Q000378:metabolism
chemical_list: D012367:RNA, Viral; D014764:Viral Proteins; D010450:Endopeptidases; C059992:nuclear inclusion protein a, mosaic viruses