[Physico-chemical properties of the thrombolytic compound longolytin].
A preparation exhibiting high fibrinolytic activity and ability to activate plasminogen was isolated from cultivation medium of Arthrobotrys longa. Homogeneous protein, obtained after gel filtration on Sephadex G-100, had molecular mass 28,600, pI-3.68-3.74, optimum activity at pH 6.0-9.0 and temperature optimum at 37 degrees. The enzyme proved to be serine proteinase as shown by analysis using inhibitors; it required thiol groups.
Tsymanovich
S A
SA
Nikandrov
V N
VN
Maksimova
R A
RA
Sharkova
T S
TS
Andreenko
G V
GV
Serebriakova
T N
TN
D002850:Chromatography, Gel; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008970:Molecular Weight; D010447:Peptide Hydrolases / Q000737:chemistry* / Q000302:isolation & purification; D010960:Plasminogen Activators / Q000737:chemistry* / Q000302:isolation & purification
D010447:Peptide Hydrolases; C037962:longolytin; D010960:Plasminogen Activators
Fiziko-khimicheskie svoĭstva tromboliticheskogo preparata longolitina.