A flexible, efficient, checkerboard immunoblot system for the detection and semiquantitation of specific antinuclear antibodies.
In this paper an uncomplicated method for the simultaneous detection and semiquantitation of 11 of the 12 commonly studied antinuclear antibodies (ANA) in a single run is described. This new application of checkerboard immunoblotting (CBIB) is based upon available technology and employs purified antigens which can be either purchased or produced in-house. CBIB requires no electronic instrument, can be formatted to meet the needs of the user, is rapidly performed, and has acceptable labor and materials costs. Data on the use of the method to examine available reference antisera is presented. CBIB has also proven practical for the clinical study of 18 sera, at two dilutions per membrane, for each set of specific antinuclear antibodies, also at two or more dilutions.
Ritchie
R F
RF
Foundation for Blood Research, Rheumatic Disease Laboratory, Scarborough, Maine.
Collins
M
M
Ledue
T B
TB
D000974:Antibodies, Antinuclear / Q000097:blood*; D000918:Antibody Specificity; D001324:Autoantigens / Q000302:isolation & purification; D001327:Autoimmune Diseases / Q000175:diagnosis / Q000276:immunology; D005069:Evaluation Studies as Topic; D006801:Humans; D015151:Immunoblotting / Q000379:methods* / Q000592:standards; D012015:Reference Standards
D000974:Antibodies, Antinuclear; D001324:Autoantigens