An ultraviolet-spectrophotometric method with 2-cyanoacetamide for the determination of the enzymatic degradation of reducing polysaccharides.
A rapid, facile, and sensitive uv-spectrophotometric assay has been developed for the determination of the enzymatic degradation of polysaccharides that generates reducing sugars. The assay was carried out with 2-cyanoacetamide in a single test tube. The solution was left at pH 9 by the addition of borate buffer within 5 min. Measurement of the reaction mixture at 274 nm allows a simple determination up to 600 mumol/liter of reducing sugars. The coefficient of variation was less than 2% on all measurements. The assay was developed with pectin and polygalacturonic acid from apples and has been compared with the Somogyi-Nelson method. The new assay was then exemplarily used for the determination of the enzymatic hydrolysis products of pectin from cotton.
Bach
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Deutsches Textilforschungszentrum Nord-West e. V., Frankenring, Germany.
Schollmeyer
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D002138:Calibration; D002384:Catalysis; D002480:Cellulase / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000378:metabolism; D006603:Hexuronic Acids / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D009570:Nitriles / Q000737:chemistry*; D010084:Oxidation-Reduction; D010368:Pectins / Q000378:metabolism; D011096:Polygalacturonase / Q000378:metabolism; D011134:Polysaccharides / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet
D006603:Hexuronic Acids; D009570:Nitriles; D011134:Polysaccharides; C007819:galacturonic acid; D010368:Pectins; D006026:Glycoside Hydrolases; C023305:hemicellulase; D011096:Polygalacturonase; D002480:Cellulase; C003181:polygalacturonic acid; C031594:2-cyanoacetamide