title: Induction and repair of UVB-induced cyclobutane pyrimidine dimers and (6-4) photoproducts in organ-cultured normal human skin.
abstract: To examine the induction and repair of UV-induced DNA damage, indirect immunofluorescence was performed on UVB-irradiated organ-cultured normal human skin using monoclonal antibodies specific for either cyclobutane pyrimidine dimers or (6-4) photoproducts. Nuclear immunofluorescence of cyclobutane pyrimidine dimers and (6-4) photoproducts were observed in a dose-dependent manner after UVB irradiation. The intensity of nuclear immunofluorescence of the upper epidermal layers was stronger and clearer than that of the lower epidermal layers. DNA repair time-course studies showed that both types of DNA damage could be repaired within 24 h after UVB irradiation.
authors.affiliation: Department of Dermatology, Nara Medical University, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Muramatsu
authors.forename: N
authors.initials: N
authors.lastname: Kobayashi
authors.forename: H
authors.initials: H
authors.lastname: Tada
authors.forename: M
authors.initials: M
authors.lastname: Yamaji
authors.forename: T
authors.initials: T
authors.lastname: Shirai
authors.forename: T
authors.initials: T
authors.lastname: Mori
authors.forename: T
authors.initials: T
authors.lastname: Ohnishi
mesh_terms: D003503:Cyclobutanes / Q000378:metabolism*; D004249:DNA Damage; D004260:DNA Repair; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D008297:Male; D008875:Middle Aged; D009924:Organ Culture Techniques; D010777:Photochemistry; D011740:Pyrimidine Dimers / Q000378:metabolism*; D012867:Skin / Q000378:metabolism / Q000528:radiation effects*; D014466:Ultraviolet Rays / Q000009:adverse effects*
chemical_list: D003503:Cyclobutanes; D011740:Pyrimidine Dimers