Synthesis and nonthrombogenicity of polymer membrane with surface-graft polymers carrying thrombin inhibitor.
An acrylamide derivative of a thrombin inhibitor was synthesized and graft polymerized to the surfaces of polymer membranes. The thrombin-inhibitor activity was unaffected by the introduction of an acryloyl group. The surface-graft membrane deactivated thrombin markedly and suppressed adhesion of platelets, resulting in a high nonthrombogenicity. Immersion of polymer membranes blended with the thrombin inhibitor in phosphate-buffered saline for 10 d resulted in the loss of nonthrombogenicity, while the polymer membranes grafted with the thrombin inhibitor derivative maintained the nonthrombogenicity over a long period.
Ito
Y
Y
Department of Polymer Chemistry, Kyoto University Yoshida Honmachi, Japan.
Liu
L S
LS
Matsuo
R
R
Imanishi
Y
Y
D000178:Acrylamides / Q000138:chemical synthesis; D000595:Amino Acid Sequence; D000818:Animals; D000991:Antithrombins / Q000494:pharmacology*; D001120:Arginine / Q000031:analogs & derivatives; D001777:Blood Coagulation / Q000187:drug effects*; D001780:Blood Coagulation Tests / Q000379:methods; D001788:Blood Grouping and Crossmatching; D004285:Dogs; D005337:Fibrin / Q000737:chemistry; D008567:Membranes, Artificial; D008969:Molecular Sequence Data; D015394:Molecular Structure; D010875:Pipecolic Acids / Q000138:chemical synthesis / Q000494:pharmacology*; D010973:Platelet Adhesiveness / Q000502:physiology; D010974:Platelet Aggregation / Q000502:physiology; D011108:Polymers / Q000009:adverse effects / Q000138:chemical synthesis*; D012996:Solutions; D013449:Sulfonamides; D013499:Surface Properties
D000178:Acrylamides; D000991:Antithrombins; D008567:Membranes, Artificial; D010875:Pipecolic Acids; D011108:Polymers; D012996:Solutions; D013449:Sulfonamides; C077760:N-(2-aminoethyl)acrylamide; D005337:Fibrin; D001120:Arginine; C031942:argatroban