Ectopic recombination within homologous immunoglobulin mu gene constant regions in a mouse hybridoma cell line.
We have transferred a pSV2neo vector containing the wild-type constant region of the immunoglobulin mu gene (C mu) into the mutant hybridoma igm482, which bears a 2-bp deletion in the third constant-region exon of its haploid chromosomal mu gene (C mu 3). Independent igm482 transformants contain the wild-type immunoglobulin C mu region stably integrated in ectopic chromosomal positions. We report here that the wild-type immunoglobulin C mu region can function as the donor sequence in a gene conversion event which corrects the 2-bp deletion in the mutant igm482 chromosomal C mu 3 exon. The homologous recombination event restores normal immunoglobulin M production in the mutant cell.
Baker
M D
MD
Department of Molecular Biology and Genetics, University of Guelph, Ontario, Canada.
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LR
D000818:Animals; D002460:Cell Line; D004591:Electrophoresis, Polyacrylamide Gel; D005785:Gene Conversion; D006825:Hybridomas; D007127:Immunoglobulin Constant Regions / Q000235:genetics*; D007074:Immunoglobulin G / Q000235:genetics; D007148:Immunoglobulin mu-Chains / Q000235:genetics*; D051379:Mice; D011995:Recombination, Genetic; D015183:Restriction Mapping; D014162:Transfection; D014170:Transformation, Genetic
D007127:Immunoglobulin Constant Regions; D007074:Immunoglobulin G; D007148:Immunoglobulin mu-Chains