[DNA-protein interactions in CpG islands and DNAse I sensitive chromatin].
DNA-protein interactions were studied in the chromatin preparations obtained according to different procedures by means of nucleoprotein-celite chromatography. Three discrete fractions dissociating in 1M, 2M and 3M NaCl were observed in all preparations. The 1M fraction prevails in DNaseI-sensitive chromatin and the 2M fraction--in the resistant. Chromatin solubilized by MspI restrictase (the active chromatin) contains the 1M and 3M fractions, one solubilized by AluI (inactive)--the 2M fraction. Distribution of the fractions is different in proliferating and quiescent cells.
S'iakste
N I
NI
S'iakste
T G
TG
Budylin
A V
AV
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000378:metabolism; D002478:Cells, Cultured; D002843:Chromatin / Q000378:metabolism*; D002845:Chromatography; D006224:Cricetinae; D004247:DNA / Q000378:metabolism*; D003850:Deoxyribonuclease I / Q000378:metabolism; D015226:Dinucleoside Phosphates / Q000235:genetics / Q000378:metabolism*; D004587:Electrophoresis, Agar Gel; D005347:Fibroblasts / Q000378:metabolism; D066298:In Vitro Techniques; D009687:Nuclear Proteins / Q000378:metabolism*; D017111:Phodopus
D002843:Chromatin; D004247:DNA; D003850:Deoxyribonuclease I; D015226:Dinucleoside Phosphates; D009687:Nuclear Proteins; C015772:CpG dinucleotide
Osobennosti DNK-belkovykh vzaimodeĭstviĭ v CPG-ostrovkakh i chuvstvitel'nom k DNKaze I khromatine.