title: Identification and properties of an atypical catalytic subunit (p34PSK-J3/cdk4) for mammalian D type G1 cyclins.
abstract: Murine D type cyclins associate with a catalytic subunit (p34PSK-J3) with properties distinct from known cyclin-dependent kinases (cdks). Mouse p34PSK-J3 shows less than 50% amino acid identity to p34cdc2, p33cdk2, and p36cdk3, lacks a PSTAIRE motif, and does not bind to p13suc1. Cyclin D1-p34PSK-J3 complexes accumulate in macrophages during G1 and decline in S phase, whereas complexes involving cyclins D2 and D3 form in proliferating T cells. Although histone H1 kinase activity is not detected in cyclin D or PSK-J3 immunoprecipitates, cyclin D-p34PSK-J3 complexes assembled in vitro stably bind and phosphorylate the retinoblastoma gene product (pRb) and an Rb-like protein (p107) but do not interact with pRb mutants that are functionally inactive. Thus, p34PSK-J3 is a cyclin D-regulated catalytic subunit that acts as an Rb (but not H1) kinase.
authors.affiliation: Howard Hughes Medical Institute, St. Jude Children's Research Hospital, Memphis, Tennessee 38105.
authors.forename: H
authors.initials: H
authors.lastname: Matsushime
authors.forename: M E
authors.initials: ME
authors.lastname: Ewen
authors.forename: D K
authors.initials: DK
authors.lastname: Strom
authors.forename: J Y
authors.initials: JY
authors.lastname: Kato
authors.forename: S K
authors.initials: SK
authors.lastname: Hanks
authors.forename: M F
authors.initials: MF
authors.lastname: Roussel
authors.forename: C J
authors.initials: CJ
authors.lastname: Sherr
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D016203:CDC2 Protein Kinase; D002478:Cells, Cultured; D019938:Cyclin D1; D051358:Cyclin-Dependent Kinase 4; D018844:Cyclin-Dependent Kinases; D016213:Cyclins / Q000737:chemistry*; D016193:G1 Phase; D006801:Humans; D007313:Insecta; D008264:Macrophages; D051379:Mice; D008969:Molecular Sequence Data; D015513:Oncogene Proteins / Q000737:chemistry*; D011494:Protein Kinases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D011518:Proto-Oncogene Proteins; D017386:Sequence Homology, Amino Acid
chemical_list: D016213:Cyclins; D015513:Oncogene Proteins; D011518:Proto-Oncogene Proteins; D019938:Cyclin D1; D011494:Protein Kinases; D016203:CDC2 Protein Kinase; C495900:CDK4 protein, human; C495902:Cdk4 protein, mouse; D051358:Cyclin-Dependent Kinase 4; D018844:Cyclin-Dependent Kinases