Virulence factors of Escherichia coli associated with colisepticemia in chickens and turkeys.
Four hundred twenty turkey and 80 chicken Escherichia coli isolates from colisepticemic birds were examined for the following properties: heat-labile toxin (LT), heat-stable enterotoxin, verotoxin, colicinogenicity, hemolysin, and hydroxamate/aerobactin production. Twenty-four (5.7%) of the 420 turkey isolates and six (7.5%) of the 80 chicken isolates produced an LT that was cytotoxic for both Vero and Y-1 cells. In contrast, 48 (11.4%) of the turkey isolates and 18 (22.5%) of the chicken isolates produced a distinct LT that was cytotoxic only for Vero cells. In addition, 64 (80.0%) of the chicken isolates and 309 (74.0%) of the turkey isolates produced aerobactin. Colicinogenicity occurred in 51 (64.0%) of the chicken isolates, with 41 (51.0%) producing colicin V. By contrast, 254 (61.0%) of the turkey isolates produced a colicin, of which 176 (42.0%) produced colicin V. None of the chicken and turkey isolates produced hemolysin or heat-stable enterotoxin.
Emery
D A
DA
Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Minnesota, St. Paul 55108.
Nagaraja
K V
KV
Shaw
D P
DP
Newman
J A
JA
White
D G
DG
D000818:Animals; D001427:Bacterial Toxins / Q000032:analysis*; D002645:Chickens / Q000382:microbiology*; D003087:Colicins / Q000032:analysis; D003108:Colonic Diseases / Q000382:microbiology / Q000662:veterinary*; D003603:Cytotoxins / Q000032:analysis; D004926:Escherichia coli / Q000737:chemistry / Q000472:pathogenicity*; D006460:Hemolysin Proteins / Q000032:analysis; D006877:Hydroxamic Acids / Q000032:analysis; D051379:Mice; D011201:Poultry Diseases / Q000382:microbiology*; D017262:Siderophores / Q000032:analysis; D014407:Tumor Cells, Cultured; D014422:Turkeys / Q000382:microbiology*; D014709:Vero Cells; D014774:Virulence
D001427:Bacterial Toxins; D003087:Colicins; D003603:Cytotoxins; D006460:Hemolysin Proteins; D006877:Hydroxamic Acids; D017262:Siderophores; C031819:aerobactin