Evaluation of sialic acid in lipoprotein fractions in fibrosarcoma bearing rats by 14C-glucose chase.
The changes in sialoglycoconjugates synthesis after administration of 14C-glucose to fibrosarcoma bearing rats were studied. After 1 hr treatment 14C-glucose tumor bearing rat synthesized hexosamine which contained more radioactivity when compared to sialic acid. On fractionation of lipoproteins, low density lipoproteins (LDL) contained more sialic acid. This was further confirmed by separating the lipoproteins, as more radioactivity incorporation was observed in the LDL fraction. It is noteworthy that inspite of the tumor, high density lipoproteins (HDL) did not show any change.
Shanmugam
V
V
Department of Microbiology, Cancer Institute Madras, India.
Nagarajan
B
B
D000818:Animals; D005354:Fibrosarcoma / Q000378:metabolism*; D005947:Glucose / Q000008:administration & dosage / Q000378:metabolism*; D006595:Hexosamines / Q000032:analysis / Q000097:blood; D008075:Lipoproteins, HDL / Q000737:chemistry*; D008077:Lipoproteins, LDL / Q000737:chemistry*; D008099:Liver / Q000737:chemistry; D019158:N-Acetylneuraminic Acid; D051381:Rats; D017208:Rats, Wistar; D012794:Sialic Acids / Q000032:analysis* / Q000097:blood
D006595:Hexosamines; D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL; D012794:Sialic Acids; D019158:N-Acetylneuraminic Acid; D005947:Glucose