Polyacrylamide as an infiltrating and embedding medium for vibratome sectioning of human fetal cerebellum containing DiI-filled axons.
Polyacrylamide is widely used for the separation of proteins by electrophoresis. Here the usage of polyacrylamide as an infiltrating and embedding medium for vibratome sectioning of human fetal cerebellum containing DiI-filled axons is reported. The procedure is simple to implement and has several advantages: it does not require prior dehydration and clearing of tissue; it can be carried out at a low temperature of 4 degrees C; and the polyacrylamide neither dissolves the DiI nor does it interfere with the visualizing of DiI-filled profiles under fluorescence microscope.
Hayaran
A
A
Department of Anatomy, All India Institute of Medical Sciences, New Delhi.
Bijlani
V
V
D000178:Acrylamides; D002232:Carbocyanines; D002531:Cerebellum / Q000033:anatomy & histology*; D005260:Female; D005333:Fetus / Q000033:anatomy & histology*; D006651:Histocytochemistry; D006735:Horseradish Peroxidase; D006801:Humans; D008867:Microtomy; D011247:Pregnancy; D016610:Tissue Embedding
D000178:Acrylamides; D002232:Carbocyanines; C024286:3,3'-dioctadecylindocarbocyanine; D006735:Horseradish Peroxidase