title: RASA contains a polymorphic microsatellite and maps to bovine syntenic group U22 on chromosome 7q2.4-qter.
abstract: The bovine gene for the p21ras protein activator (RASA) includes in its 5' untranslated region a (TG)n repeat. Analysis of this (TG)n repeat by PCR amplification of genomic DNA revealed a four-allele polymorphism. A cDNA probe was used to assign RASA to the region 2.4-qter of bovine Chromosome (Chr) 7 by in situ hybridization. PCR analysis of a panel of somatic hybrid lines allowed the assignment of RASA to the unassigned syntenic group 22 (U22) and thus localizes U22 on Chr 7.
authors.affiliation: Department of Animal Science, Swiss Federal Institute of Technology, Zurich.
authors.forename: A
authors.initials: A
authors.lastname: Eggen
authors.forename: S
authors.initials: S
authors.lastname: Solinas-Toldo
authors.forename: A B
authors.initials: AB
authors.lastname: Dietz
authors.forename: J E
authors.initials: JE
authors.lastname: Womack
authors.forename: G
authors.initials: G
authors.lastname: Stranzinger
authors.forename: R
authors.initials: R
authors.lastname: Fries
mesh_terms: D000818:Animals; D001483:Base Sequence; D016466:CHO Cells; D002417:Cattle; D002871:Chromosome Banding; D002874:Chromosome Mapping; D006224:Cricetinae; D004276:DNA, Satellite / Q000235:genetics*; D006822:Hybrid Cells; D017403:In Situ Hybridization; D008297:Male; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D011506:Proteins / Q000235:genetics* / Q000378:metabolism; D016283:Proto-Oncogene Proteins p21(ras) / Q000378:metabolism
chemical_list: D004276:DNA, Satellite; D011506:Proteins; D016283:Proto-Oncogene Proteins p21(ras)