Formation of oligosaccharides from lactitol by Aspergillus oryzae beta-D-galactosidase.
Six oligosaccharides were first formed from lactitol by a transgalactosylation reaction catalyzed by Aspergillus oryzae beta-D-galactosidase. From the results of methylation analysis, MS, and 1H- and 13C-NMR studies, it was concluded that these oligosaccharides are O-beta-D-galactopyranosyl-(1----4)-O-beta-D-galactopyranosyl-(1----4)-D- glucitol, O-beta-D-galactopyranosyl-(1----3)-O-beta-D-galactopyranosyl-(1----4)-D- glucitol, O-beta-D-galactopyranosyl-(1----4)-[O-beta-D-galactopyranosyl-(1----6)]- D- glucitol, O-beta-D-galactopyranosyl-(1----6)-O-beta-D-galactopyranosyl-(1---4)- glucitol, O-beta-D-galactopyranosyl-(1----4)-[O-beta-D-galactopyranosyl- (1----5)]-D-glucitol, and O-beta-D-galactopyranosyl-(1----4)-[O-beta-D-galactopyranosyl-(1----1)]- D-glucitol. The last three are newly observed oligosaccharides.
Yanahira
S
S
Technical Research Institute, Snow Brand Milk Products Co., Ltd., Saitama, Japan.
Suguri
T
T
Yakabe
T
T
Ikeuchi
Y
Y
Hanagata
G
G
Deya
E
E
D001236:Aspergillus oryzae / Q000737:chemistry / Q000201:enzymology*; D002240:Carbohydrate Sequence; D002384:Catalysis; D006031:Glycosylation; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification; D013402:Sugar Alcohols / Q000737:chemistry*; D001616:beta-Galactosidase / Q000737:chemistry*
D009844:Oligosaccharides; D013402:Sugar Alcohols; D001616:beta-Galactosidase; C014635:lactitol