Continuous measurement of lactate concentration in skeletal muscle and liver interstitium using a microdialysis method after acute hemorrhage in anesthetized rats.
Interstitial lactate concentration in rat skeletal muscle was continuously measured using a microdialysis method following an acute hemorrhage. For comparison, similar measurements were also performed for liver. Normal lactate levels of skeletal muscle and liver interstitium were 0.58 +/- 0.10 mM and 1.23 +/- 0.04 mM, respectively. After withdrawal of 30% of the estimated total blood volume within 3 min, a constant high blood lactate level was achieved. The lactate concentrations in muscle and liver interstitium also increased to 270 and 210% of the basal levels, respectively, but they did not exceed the blood level. These findings led us to hypothesize that skeletal muscle as well as liver functions as a lactate consumer rather than a producer after acute hemorrhage.
Okuda
C
C
Department of Anesthesiology, Kyoto Prefectural University of Medicine, Japan.
Sawa
T
T
Harada
M
M
Murakami
T
T
Tanaka
Y
Y
D000818:Animals; D001786:Blood Glucose; D003956:Dialysis / Q000379:methods; D004195:Disease Models, Animal; D006470:Hemorrhage / Q000378:metabolism*; D007773:Lactates / Q000032:analysis*; D008099:Liver / Q000378:metabolism*; D008297:Male; D009132:Muscles / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D012039:Regional Blood Flow
D001786:Blood Glucose; D007773:Lactates