Elimination of sample diffusion and lateral band spreading in isoelectric focusing employing ready-made immobilized pH gradient gels.
A simple procedure for the elimination of lateral sample as well as band spreading in precast, ready-made immobilized pH gradient gels is described. Round or rectangular holes are punched in the dry polyacrylamide gels prior to rehydration. The generated wells proved suitable for application of samples containing surfactants such as Nonidet P-40 or 3-[(3-cholamidopropyl) dimethylammonio]-1- propane-sulfonate (CHAPS). Lateral band spreading and precipitation of samples containing up to 9.5 M urea could be completely eliminated by this method.
Loessner
M J
MJ
Institut für Mikrobiologie, Technische Universität München, Germany.
Scherer
S
S
D002793:Cholic Acids; D004058:Diffusion; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing / Q000379:methods*; D017830:Octoxynol; D011092:Polyethylene Glycols; D011506:Proteins / Q000302:isolation & purification*; D011786:Quality Control
D002793:Cholic Acids; D011506:Proteins; D011092:Polyethylene Glycols; D017830:Octoxynol; C010615:Nonidet P-40; C028213:3-((3-cholamidopropyl)dimethylammonium)-1-propanesulfonate