EAE cerebrospinal fluid augments in vitro phagocytosis and metabolism of CNS myelin by macrophages.
Previous studies from this laboratory have shown that CNS myelin is phagocytized and metabolized by cultured rat macrophages to a much larger extent when myelin is pretreated with serum containing antibodies to myelin constituents than when it is left untreated or pretreated with non-specific serum. In this study the effect of cerebrospinal fluid (CSF) from rabbits with experimental allergic encephalomyelitis (EAE) in promoting myelin phagocytosis was examined. Fourteen rabbits were immunized with purified myelin in Freund's complete adjuvant, seven of which developed clinical EAE symptoms. Serum and CSF were collected from EAE and control rabbits, and the CSF was centrifuged to remove cells. Sera and CSF from these rabbits and from Freund's adjuvant-immunized controls and untreated controls were measured for IgG content by radial diffusion assay, their myelin antibody characteristics were analyzed by immunoblots, and the ability of these serum and CSF samples to promote myelin phagocytosis when used for myelin opsonization was examined. The ability of a CSF sample to enhance radioactive myelin uptake and phagocytosis by cultured macrophages as measured by the appearance of radioactive cholesterol ester was linearly proportional to its total IgG titer, and correlated approximately both with clinical symptoms of the animal and the presence of antibody against the myelin constituents myelin basic protein, proteolipid protein, and galactocerebroside. The cholesterol esterification activities of EAE sera correlated to a lesser extent with IgG levels and clinical symptoms.(ABSTRACT TRUNCATED AT 250 WORDS)
Sommer
M A
MA
Department of Neurology, Veterans Affairs Medical Center, Palo Alto, CA 94304.
Forno
L S
LS
Smith
M E
ME
D000818:Animals; D002490:Central Nervous System / Q000378:metabolism*; D002788:Cholesterol Esters / Q000276:immunology / Q000378:metabolism; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000134:cerebrospinal fluid*; D005260:Female; D005612:Freeze Drying; D015151:Immunoblotting; D005779:Immunodiffusion; D007074:Immunoglobulin G / Q000276:immunology; D008055:Lipids / Q000302:isolation & purification; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D009186:Myelin Sheath / Q000378:metabolism*; D010587:Phagocytosis / Q000187:drug effects*; D011817:Rabbits
D002788:Cholesterol Esters; D007074:Immunoglobulin G; D008055:Lipids