Subcellular distribution of hydralazine in rat single vascular muscle cells.
High specific activity (20 Ci/mmol) tritiated hydralazine (3Hyd) distribution in isolated, cultured vascular muscle cells was determined to identify the sites of Hyd binding. 3Hyd dose-dependently bound to extracellular protein and to the area of organelles which secrete these proteins. Increased extracellular binding after Hyd pre-exposure suggests new binding sites may be exacerbated as a result of Hyd interactions. These experiments suggest a potentially important feature of the mechanism of action of this directly acting vasodilator.
Baker
J R
JR
Department of Medicine, Oregon Health Sciences University, Portland 97213.
Hedwall
P R
PR
Hermsmeyer
K
K
D000818:Animals; D001011:Aorta / Q000166:cytology; D002478:Cells, Cultured; D006830:Hydralazine / Q000032:analysis / Q000378:metabolism / Q000494:pharmacology*; D008854:Microscopy, Electron; D009119:Muscle Contraction / Q000187:drug effects*; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D011869:Radioligand Assay; D051381:Rats
D006830:Hydralazine