Phospholipase A2 in rat gingival tissue.
Phospholipase A2 (PLA2) is a proinflammatory enzyme in the synovial fluids of all--and sera of some--patients with rheumatoid arthritis. Due to the similarities in pathogenesis between rheumatoid arthritis and periodontitis, we sought to study the enzymatic properties of PLA2 in periodontal tissue. In this study, we demonstrated PLA2 activity in rat gingival tissue, about 80% of which was present in the cytosolic fraction. We characterized the cytosolic PLA2 enzyme with respect to substrate specificity, sensitivity to detergent, Ca2+ ion dependency and optimum pH. We found that phosphatidylethanolamine, rather than phosphatidylcholine, was the preferred substrate, the Ca2+ ion was essential for the expression of PLA2 activity, the enzyme was active over a broad pH range, with the optimum at pH 9.0, and sodium-deoxycholate inhibited the enzyme activity strongly in a concentration-dependent manner. These results are consistent with those which have been obtained with synovial fluid PLA2 and suggest that gingival PLA2 may be involved in the pathogenic processes of gingivitis and periodontitis.
Shinohara
H
H
Department of Periodontology and Endodontology, School of Dentistry, Tokushima University, Japan.
Ishida
H
H
Fernandez
E J
EJ
Amabe
Y
Y
Nagata
T
T
Wakano
Y
Y
D000704:Analysis of Variance; D000818:Animals; D002118:Calcium / Q000378:metabolism; D003600:Cytosol / Q000201:enzymology; D003840:Deoxycholic Acid / Q000494:pharmacology; D004789:Enzyme Activation; D005881:Gingiva / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D008297:Male; D010713:Phosphatidylcholines / Q000378:metabolism; D010714:Phosphatidylethanolamines / Q000378:metabolism; D010741:Phospholipases A / Q000032:analysis / Q000037:antagonists & inhibitors / Q000378:metabolism*; D054467:Phospholipases A2; D051381:Rats; D017208:Rats, Wistar; D013379:Substrate Specificity
D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D003840:Deoxycholic Acid; D010741:Phospholipases A; D054467:Phospholipases A2; D002118:Calcium