title: MIPSY: real-time morphometry to quantify the time course of rapid epithelial restitution.
abstract: The gastrointestinal mucosa has the ability to repair itself rapidly following superficial mucosal injury by rapid epithelial restitution. The mechanism consists of cell migration and does not involve mitosis. This study reports the mechanisms of rapid epithelial restitution in the rabbit colon in vivo and in the human colon in vitro, describes a new computerized real-time morphometry system to investigate the time course of restitution and presents a new method to calculate the migration speed of epithelia during the repair process. Superficial mucosal damage to the rabbit colon in vivo was produced by luminal exposure to 100 mM HCl for 5 min (80% of mucosal surface), a comparable injury in the human colon in vitro was obtained by luminal exposure to 10 mM HCl for 10 min (96% of mucosal surface). After detachment of the damaged tissue the intact epithelial cells in the vicinity of the necrosis extended pseudopodia and migrated over the denuded basal lamina. The morphological appearance of rapid epithelial restitution was the same in the rabbit and the human, only the time course was postponed in the human. The time course of rapid restitution was assessed by a newly developed computerized morphometry system (MIPSY). When tissues were examined after various time points following acid damage, 61% of the mucosa were damaged in the rabbit after 1 h, 10% after 2 hs and 20% after 5 hs. In the human colon 85% of the mucosal surface were damaged after 2 hs and 20% 5 hs after the end of acid exposure.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Surgical Basic Research Laboratory, University Clinic Surgery I, Vienna, Austria.
authors.forename: M
authors.initials: M
authors.lastname: Riegler
authors.forename: W
authors.initials: W
authors.lastname: Feil
authors.forename: J
authors.initials: J
authors.lastname: Lindroth
authors.forename: M
authors.initials: M
authors.lastname: Wong
mesh_terms: D000818:Animals; D002465:Cell Movement / Q000502:physiology; D003106:Colon / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D004847:Epithelial Cells; D004848:Epithelium / Q000187:drug effects / Q000502:physiology; D005260:Female; D006801:Humans; D006851:Hydrochloric Acid / Q000494:pharmacology; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D007413:Intestinal Mucosa / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D011817:Rabbits; D012038:Regeneration; D013997:Time Factors; D014945:Wound Healing / Q000502:physiology*
chemical_list: D006851:Hydrochloric Acid