Mutation in the specificity polypeptide of the type I restriction endonuclease R.EcoK that affects subunit assembly.
We describe the isolation and characterization of a temperature-sensitive mutation within the hsdS gene of the type I restriction and modification system EcoK. This mutation appears to affect the ability of the HsdR subunit to interact with the HsdS subunit when forming an active endonuclease. We discuss the possibility that this mutant, together with another mutation described previously, may define a discontinuous domain, involved in protein-protein interactions, within the HsdS polypeptide.
Zinkevich
V
V
Department of Biochemistry and Genetics, Medical School, University of Newcastle-upon-Tyne, U.K.
Heslop
P
P
Glover
S W
SW
Weiserova
M
M
Hubácek
J
J
Firman
K
K
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D015253:Deoxyribonucleases, Type I Site-Specific / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D005798:Genes, Bacterial / Q000235:genetics; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D010957:Plasmids / Q000235:genetics; D015183:Restriction Mapping; D013696:Temperature
D046911:Macromolecular Substances; C029602:endodeoxyribonuclease EcoK; D015253:Deoxyribonucleases, Type I Site-Specific