title: Experimental polyacrylamide-induced acute injury in rat lung.
abstract: We recently reported the first case of accidental aspiration of polyacrylamide occurring in a 26-year-old man. The patient developed severe airway obstruction and parenchymal lung damage and died. Autopsy revealed numerous polyacrylamide particles in his lungs, as well as extensive bronchiolar and alveolar damage. Gas chromatographic and mass spectrometric assessment of the lung tissue failed to reveal polyacrylamide activity, although assessment of the suspending solvent of the polyacrylamide showed a pattern characteristic of an aliphatic hydrocarbon mixture with a prominent dodecane peak. This experimental study was performed to determine the nature and extent of damage to rat bronchial and alveolar epithelia following endotracheal instillation of polyacrylamide, hydrocarbon mixture (petroleum distillate), dodecane (C12H26), or normal saline. The rat lungs were examined grossly and microscopically 10 min and 24, 72, and 96 h after endotracheal instillation, following inflation and fixation with 10 percent buffered formaldehyde. Gross examination revealed congested, mottled visceral pleural surfaces in the rats treated with polyacrylamide and dodecane. There were no pleural exudates or effusions. Microscopically, vascular engorgement, bronchiolitis, and focal pneumonia were observed. Vascular engorgement was most pronounced at 72 to 96 h in rat lungs treated with polyacrylamide and dodecane and was moderate at 24 h in rats treated with petroleum distillate. Focal organizing pneumonia was marked at 96 h in rats treated with petroleum distillate, at 72 h in those treated with polyacrylamide, and at 24 h in those treated with dodecane. The saline-treated control animals showed no change. Our findings suggest that polyacrylamide, dodecane, and petroleum distillate are strong irritants to the airways. However, a direct obstructive/mechanical effect of the polyacrylamide upon the airway has not been excluded. Airway exposure to polyacrylamide may result in lung injury secondary to the polyacrylamide itself, its suspending agents, or both.
authors.affiliation: Department of Pathology, Baylor College of Medicine, Houston.
authors.forename: A E
authors.initials: AE
authors.lastname: Fraire
authors.forename: I
authors.initials: I
authors.lastname: Shahab
authors.forename: S D
authors.initials: SD
authors.lastname: Greenberg
authors.forename: A
authors.initials: A
authors.lastname: Jubran
authors.forename: M
authors.initials: M
authors.lastname: Noall
mesh_terms: D000180:Acrylic Resins / Q000008:administration & dosage / Q000633:toxicity*; D000208:Acute Disease; D000473:Alkanes / Q000633:toxicity; D000818:Animals; D005260:Female; D001239:Inhalation; D008168:Lung / Q000187:drug effects / Q000473:pathology*; D008171:Lung Diseases / Q000139:chemically induced* / Q000473:pathology; D010578:Petroleum / Q000633:toxicity; D051381:Rats; D011916:Rats, Inbred F344; D000099166:Polyacrylamides
chemical_list: D000180:Acrylic Resins; D000473:Alkanes; D010578:Petroleum; D000099166:Polyacrylamides; C007548:n-dodecane