Carcinogen-specific mutational pattern in the p53 gene in ultraviolet B radiation-induced squamous cell carcinomas of mouse skin.
We have examined 35 epidermal tumors induced in mice of four different strains by chronic exposure to ultraviolet B radiation for the presence of aberrations in the p53 tumor suppressor gene. Polymerase chain reaction products from p53 exons 5 to 8 were screened by single-strand conformation polymorphism analysis and sequencing. Base substitutions were found in seven tumors (20%). All mutations occurred at dipyrimidine sequences; most frequent were C-->T single base and CC-->TT tandem transitions suggesting the involvement of UV radiation in the genesis of the mutations. Three base substitutions were located at codon 148, and all dipyrimidine-derived mutations occurred at sites where the sequence is present in the nontranscribed DNA strand, indicating some site and strand specificity of the ultraviolet B-induced p53 mutations.
Kress
S
S
German Cancer Research Center, Project Group Tumor Promotion in Liver, Heidelberg.
Sutter
C
C
Strickland
P T
PT
Mukhtar
H
H
Schweizer
J
J
Schwarz
M
M
D000818:Animals; D001483:Base Sequence; D002294:Carcinoma, Squamous Cell / Q000209:etiology / Q000235:genetics*; D003062:Codon / Q000528:radiation effects; D004273:DNA, Neoplasm / Q000235:genetics / Q000528:radiation effects; D005091:Exons / Q000528:radiation effects; D016158:Genes, p53 / Q000235:genetics* / Q000528:radiation effects; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008969:Molecular Sequence Data; D009154:Mutation; D009381:Neoplasms, Radiation-Induced / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012878:Skin Neoplasms / Q000209:etiology / Q000235:genetics*; D014466:Ultraviolet Rays / Q000009:adverse effects*
D003062:Codon; D004273:DNA, Neoplasm