Chromosomal and genetic analysis of the electrophoretic karyotype of Trichoderma reesei: mapping of the cellulase and xylanase genes.
An electrophoretic karyotype has been established for Trichoderma reesei strain QM6a, and several of its derivatives, by pulsed-field gel electrophoresis. All strains examined appear to have seven chromosomes with a total genome size of approximately 33 megabases (Mb). The sizes of the chromosomal bands in strain QM6a are approximately 6.2, 6.0, 5.1, 4.2 (doublet), 3.6 and 3.2 Mb. Genes encoding the cellulase complex and xylanases of T. reesei have been mapped, as have several other genes.
Carter
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Genencor International, South San Francisco, California 94080.
Allison
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Rey
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Dunn-Coleman
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D002480:Cellulase / Q000235:genetics*; D002874:Chromosome Mapping; D015825:Chromosomes, Fungal / Q000737:chemistry*; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods; D005656:Fungal Proteins / Q000235:genetics*; D005800:Genes, Fungal; D008040:Genetic Linkage; D006026:Glycoside Hydrolases / Q000235:genetics*; D007621:Karyotyping; D015183:Restriction Mapping; D014242:Trichoderma / Q000201:enzymology / Q000235:genetics*; D043325:Xylan Endo-1,3-beta-Xylosidase
D005656:Fungal Proteins; D006026:Glycoside Hydrolases; D043325:Xylan Endo-1,3-beta-Xylosidase; D002480:Cellulase