Determination of vinorelbine (Navelbine) in tumour cells by high-performance liquid chromatography.
A high-performance liquid chromatographic method has been developed for the determination within tumour cells of a new vinca alkaloid, vinorelbine. Extractions of vinorelbine from cells were carried out using absolute ethanol. The extracts were injected into a reversed-phase system consisting of two Novapak C18 columns connected in series. The mobile phase was acetonitrile-phosphate buffer, pH 2.7 (60:40, v/v). Using a fluorescence detection, the limit of determination was 8 pmol injected. This method would be suitable for studying the cellular pharmacokinetics of vinorelbine in patients.
Debal
V
V
Laboratoire de Spectroscopie Biomoléculaire, U.F.R. de Pharmacie, Reims, France.
Morjani
H
H
Millot
J M
JM
Angiboust
J F
JF
Gourdier
B
B
Manfait
M
M
D000970:Antineoplastic Agents / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007951:Leukemia, Myeloid; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence; D014407:Tumor Cells, Cultured / Q000737:chemistry*; D014747:Vinblastine / Q000031:analogs & derivatives* / Q000032:analysis; D000077235:Vinorelbine
D000970:Antineoplastic Agents; D014747:Vinblastine; D000077235:Vinorelbine