[Induction of interleukin-1 production in the cultured synovial cells from patients with rheumatoid arthritis].
Interleukin-1 (IL-1) is believed to be involved in articular destruction in rheumatoid arthritis. HLA-class II antigens are expressed on synovial cells of patients with RA. The relation between the production of IL-1 and expression of HLA-class II antigens was studied. Synovial cells of rheumatoid patients appeared to express HLA-DR and DQ antigens to a significantly greater extent than those of osteoarthritic patients. These cells produced IL-1 following interferon-gamma (IFN-gamma) stimulation and there was synergistic enhancement of production induced by IFN-gamma and monoclonal antibodies to HLA-DR or DQ antigens in combination. In the intracellular signal transduction mechanism for the production of IL-1 beta by these cells following IFN-gamma stimulation, protein kinase C and calmodulin may be involved as second messengers.
Ikuta
Y
Y
Department of Orthopedic Surgery, Hamamatsu University School of Medicine.
Miyamoto
S
S
Inoue
T
T
Koide
Y
Y
Yoshida
T
T
D001172:Arthritis, Rheumatoid / Q000276:immunology / Q000378:metabolism*; D002478:Cells, Cultured; D000949:Histocompatibility Antigens Class II / Q000378:metabolism; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology; D007375:Interleukin-1 / Q000096:biosynthesis*; D015398:Signal Transduction; D013583:Synovial Membrane / Q000276:immunology / Q000378:metabolism*
D000949:Histocompatibility Antigens Class II; D007375:Interleukin-1; D007371:Interferon-gamma