title: Androgenic control of polyamine concentrations in rat epididymis.
abstract: Unilateral orchidectomy resulted in a significant decrease in tissue content of putrescine and polyamines. However, no differences were detected when the results were expressed in terms of ng g-1 tissue. At 48 h after bilateral orchidectomy, a significant decrease in putrescine content was observed, but spermidine and spermine content were unaffected. The observed decrease in putrescine was prevented by treatment with testosterone propionate, but neither spermidine nor spermine were affected. Bilateral orchidectomy resulted in a significant decrease in the tissue content of putrescine, spermidine and spermine after 7 days. Treatment with testosterone propionate increased the content of putrescine, spermidine and spermine in the epididymis by about 200%, 92% and 34%, respectively. When results were expressed as nmol g-1, a significant decrease after castration in putrescine and spermidine, but not in spermine, was observed. Treatment with testosterone propionate restored putrescine concentration, but had no effect on spermidine and spermine concentrations. In castrated rats treated with testosterone propionate, the anti-androgen flutamide abolished the effect of the androgen on putrescine and spermidine content, but there was no effect on spermine. Acetylputrescine was not detected in the epididymis, while acetylpolyamines were detected at much lower concentrations than polyamines. After bilateral orchidectomy there was a decrease in the tissue content of all acetylpolyamines and an increase in their tissue concentration. The effect of castration on acetylpolyamine content was reversed by testosterone propionate treatment. We conclude that an active synthesis of polyamines occurs in the rat epididymis, and that this process depends upon the androgen environment. Regulation of ornithine decarboxylase activity appears to be the main step that is controlled by androgens.
authors.affiliation: Laboratorio de Esteroides, Instituto de Biología y Medicina Experimental, Buenos Aires, Argentina.
authors.forename: M A
authors.initials: MA
authors.lastname: de las Heras
authors.forename: S I
authors.initials: SI
authors.lastname: Gonzalez
authors.forename: R S
authors.initials: RS
authors.lastname: Calandra
mesh_terms: D000728:Androgens / Q000502:physiology*; D000818:Animals; D004822:Epididymis / Q000187:drug effects / Q000378:metabolism*; D005485:Flutamide / Q000494:pharmacology; D008297:Male; D009919:Orchiectomy; D011073:Polyamines / Q000378:metabolism*; D011700:Putrescine / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013095:Spermidine / Q000378:metabolism; D013096:Spermine / Q000378:metabolism; D013739:Testosterone / Q000494:pharmacology
chemical_list: D000728:Androgens; D011073:Polyamines; D013096:Spermine; D013739:Testosterone; D005485:Flutamide; D013095:Spermidine; D011700:Putrescine