title: The 66 kDa component of yeast SFI, stimulatory factor I, is hsp60.
abstract: DNA polymerase epsilon stimulatory factor I (SFI) has been shown to contain three peptides, p66, p37 and p13. Two of these components have been identified. The p66 gene was cloned by using a p66 antibody to screen a lambda gt11 library. A portion of the gene was sequenced and confirmed to encode p66 by the presence of protein sequence corresponding to that of p66 tryptic peptides. The gene was identified as HSP60 by a homology search of GenBank. Tryptic peptides of p37 were sequenced and identified as belonging to yeast translation initiation factor 4A by a homology search of PIR. The HSP60 gene maps to chromosome XII.
authors.affiliation: Braun Laboratories, Division of Chemistry, California Institute of Technology, Pasadena 91125.
authors.forename: J K
authors.initials: JK
authors.lastname: Smiley
authors.forename: W C
authors.initials: WC
authors.lastname: Brown
authors.forename: J L
authors.initials: JL
authors.lastname: Campbell
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D018834:Chaperonin 60; D018833:Chaperonins; D002874:Chromosome Mapping; D015825:Chromosomes, Fungal; D003001:Cloning, Molecular; D004271:DNA, Fungal / Q000235:genetics / Q000302:isolation & purification; D039562:Eukaryotic Initiation Factor-4F; D005800:Genes, Fungal; D006360:Heat-Shock Proteins / Q000235:genetics* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D020365:RNA Helicases; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D017386:Sequence Homology, Amino Acid
chemical_list: D018834:Chaperonin 60; C077121:DNA polymerase epsilon stimulatory factor 1, S cerevisiae; D004271:DNA, Fungal; D039562:Eukaryotic Initiation Factor-4F; C504495:HSP60 protein, S cerevisiae; D006360:Heat-Shock Proteins; D009838:Oligodeoxyribonucleotides; D029701:Saccharomyces cerevisiae Proteins; C504497:TIF2 protein, S cerevisiae; D018833:Chaperonins; D020365:RNA Helicases; C504496:TIF1 protein, S cerevisiae