Measurement of dothiepin and its major metabolites in plasma by high-performance liquid chromatography.
This paper describes a reversed-phase high-performance liquid chromatographic method which will simultaneously measure dothiepin and its three major metabolites (northiaden, northiaden-S-oxide and dothiepin-S-oxide) in plasma using trimipramine as internal standard. Sample preparation involved a basic extraction using diethyl ether followed by an acid back-extraction. The method we report is linear over the range 50-1000 ng/ml (r = 0.999), for all analytes. Total imprecision is less than 11% (coefficient of variation) and accuracy is greater than 94% (n = 20). Recovery of analytes varied considerably from 51.7% for northiaden-S-oxide to 90.2% for dothiepin-S-oxide.
Taylor
P J
PJ
Department of Clinical Pharmacology, Princess Alexandra Hospital, Woolloongabba, Queensland, Australia.
Charles
B G
BG
Norris
R
R
Salm
P
P
Ravenscroft
P J
PJ
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004308:Dothiepin / Q000097:blood*; D006801:Humans; D012015:Reference Standards; D015203:Reproducibility of Results; D014299:Trimipramine / Q000097:blood
D014299:Trimipramine; D004308:Dothiepin