title: A perfused-cuvette method for fluorimetric studies of non-adherent cells.
abstract: We have developed a system for immobilizing non-adherent cells for use in macroscopic fluorescence measurements in a perfused cuvette. Using normal human T lymphocytes loaded with the fluorescent Ca(2+)-indicator, fluo-3, we have validated the properties of these cells immobilized on clear, non-fluorescent fluorohalocarbon film (Aclar) using the non-charged cell adhesive, Cell-Tak.
authors.affiliation: Playfair Neuroscience Unit, Toronto Hospital, Ontario, Canada.
authors.forename: L C
authors.initials: LC
authors.lastname: Schlichter
authors.forename: P S
authors.initials: PS
authors.lastname: Pennefather
authors.forename: C J
authors.initials: CJ
authors.lastname: van Staden
authors.forename: E A
authors.initials: EA
authors.lastname: Valentine
mesh_terms: D002469:Cell Separation / Q000379:methods*; D006801:Humans; D010477:Perfusion / Q000379:methods*; D013050:Spectrometry, Fluorescence / Q000379:methods; D013601:T-Lymphocytes