Cell-specific expression of LBP-32 mRNA in retina and other locations of newborn mouse eye as revealed by in situ hybridization.
LBP-32 is a cell surface and cytoplasmic protein which is thought to both mediate cell attachment to laminin and play a role in translation initiation. In the present study, antisense RNA for LBP-32 was used to document its cellular mRNA expression pattern in newborn mouse eye. In situ hybridization revealed that LBP-32 was distributed uniformly through the retina as well as over anterior oblique muscle, in corneal and lens epithelial cells and in capillary endothelial cells of the choroid. This unique cell-specific expression raises interesting questions of the role of LBP-32 in eye development.
Stone
C M
CM
Department of Anatomy and Cell Biology, University of Virginia, Charlottesville.
Laurie
G W
GW
D000818:Animals; D000831:Animals, Newborn; D002829:Choroid / Q000098:blood supply; D003315:Cornea / Q000378:metabolism; D004730:Endothelium, Vascular / Q000378:metabolism; D004848:Epithelium / Q000378:metabolism; D005123:Eye / Q000378:metabolism*; D015870:Gene Expression; D017403:In Situ Hybridization; D007797:Laminin / Q000235:genetics* / Q000378:metabolism*; D007908:Lens, Crystalline / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D009801:Oculomotor Muscles / Q000378:metabolism; D016372:RNA, Antisense; D012333:RNA, Messenger / Q000378:metabolism*; D012160:Retina / Q000378:metabolism*
D007797:Laminin; D016372:RNA, Antisense; D012333:RNA, Messenger