Deletion of chromosome 11p13-11p15.5 sequences in invasive human ovarian cancer is a subclonal progression factor.
In human ovarian cancer, multiple specific chromosomal deletions can be found by cytogenetic analysis or molecular techniques such as restriction fragment length polymorphism probing. This work confirms the loss of HRAS alleles in 1 out of 2 cases of invasive ovarian cancer as determined in 32 samples or cell lines derived from 19 patients. Results with other polymorphic probes indicate that a consensus deletion probably includes 11p15.5-11p13. Tumor suppressor genes might be located in the deleted area, and deletion of the gene might then play a role in disease progression. Examination of DNA from distinct tumor sites of individual patients indicates clonal heterogeneity in the malignant cell population, indicating that loss of 11p sequences is a late event in the disease progression. Loss of alternate 11p alleles at different disease sites in one patient is inconsistent with the current model of tumor suppressor gene inactivation. The 11p deletion seems to be limited to ovarian cancers in younger patients. Eight novel permanent ovarian cancer cell lines, previously not described in the literature and derived from tumor sites from five patients, were included in the current analysis.
Vandamme
B
B
Laboratorium Medische Genetika, Akademisch Ziekenhuis, Vrije Universiteit Brussel, Jette, Belgium.
Lissens
W
W
Amfo
K
K
De Sutter
P
P
Bourgain
C
C
Vamos
E
E
De Grève
J
J
D000328:Adult; D000368:Aged; D000483:Alleles; D002872:Chromosome Deletion; D002880:Chromosomes, Human, Pair 11; D004273:DNA, Neoplasm / Q000032:analysis*; D005260:Female; D016153:Genes, Suppressor; D006580:Genetic Carrier Screening; D006801:Humans; D008875:Middle Aged; D010051:Ovarian Neoplasms / Q000235:genetics* / Q000473:pathology
D004273:DNA, Neoplasm