Breakpoints at 11q23 in infant leukemias with the t(11;19)(q23;p13) are clustered.
We have analyzed a series of nine infant leukemias that carry a t(11;19)(q23;p13). They had the morphologic features of acute lymphoblastic leukemia (ALL) and expressed markers typical of B-cell progenitor ALL or pre-B ALL; one coexpressed myeloid markers in addition to lymphoid markers (biphenotypic). Two probes (P/S4 and 98.40) subcloned from a yeast artificial chromosome (YAC) known to span the breakpoint in the t(4;11) were used to investigate DNA isolated from the leukemic cells of these patients. A total of approximately 15 kb of genomic DNA in the vicinity of the probes was examined by conventional Southern blot analysis using a series of restriction enzymes. In eight of the nine cases, the breakpoint could be mapped to an approximately 10-kb BamHI fragment disclosed by hybridization to the P/S4 probe.
Morgan
G J
GJ
Leukaemia Research Fund Centre, Institute of Cancer Research, Chester Beatty Laboratories, London, UK.
Cotter
F
F
Katz
F E
FE
Ridge
S A
SA
Domer
P
P
Korsmeyer
S
S
Wiedemann
L M
LM
D015703:Antigens, CD / Q000032:analysis; D015139:Blotting, Southern; D002648:Child; D002675:Child, Preschool; D002874:Chromosome Mapping; D002880:Chromosomes, Human, Pair 11; D002888:Chromosomes, Human, Pair 19; D004273:DNA, Neoplasm / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D006684:HLA-DR Antigens / Q000032:analysis; D006801:Humans; D007223:Infant; D008297:Male; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000145:classification / Q000235:genetics* / Q000276:immunology; D015183:Restriction Mapping; D014178:Translocation, Genetic
D015703:Antigens, CD; D004273:DNA, Neoplasm; D006684:HLA-DR Antigens