[The inhibition by the tumor necrosis factor of the death and DNA fragmentation of lymphoid cells in irradiated rats].
The influence of a tumor necrosis factor, administered 16 h before irradiation of rats, on the radiation response of thymus and bone marrow cells has been investigated. Three and 6 h after irradiation the following indices were analyzed: the number of apoptotic cells in the thymus; the accumulation of polydeoxyribonucleotides and the appearance of single-strand breaks in DNA of bone marrow and thymus cells; and the electrophoretic properties of thymocyte DNA. The injection of a tumor necrosis factor reduced the number of polydeoxyribonucleotides, inhibited internucleosome DNA fragmentation, and did not influence the formation of single-strand breaks in DNA.
Proskuriakov
S Ia
SIa
Ivannik
B P
BP
Aleksandrova
S E
SE
Sin'kova
R V
RV
Degtiareva
E A
EA
Riabchenko
N I
NI
D000818:Animals; D001853:Bone Marrow / Q000187:drug effects / Q000528:radiation effects / Q000648:ultrastructure; D016923:Cell Death / Q000187:drug effects / Q000528:radiation effects; D004249:DNA Damage; D004277:DNA, Single-Stranded / Q000187:drug effects* / Q000528:radiation effects; D003864:Depression, Chemical; D005720:Gamma Rays; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects* / Q000528:radiation effects / Q000648:ultrastructure; D008297:Male; D011089:Polydeoxyribonucleotides / Q000528:radiation effects; D051381:Rats; D011994:Recombinant Proteins / Q000494:pharmacology; D013950:Thymus Gland / Q000187:drug effects / Q000528:radiation effects / Q000648:ultrastructure; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
D004277:DNA, Single-Stranded; D011089:Polydeoxyribonucleotides; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha
Ingibirovanie faktorom nekroza opukholi gibeli i fragmentatsii DNK limfoidnykh kletok obluchennykh krys.