High resolution two-dimensional mapping of tissue-specific polypeptides in the desert locust, Schistocerca gregaria.
High resolution two-dimensional gel electrophoresis (2-DE) using immobilized pH gradients was used to map the tissue-specific polypeptides of the desert locust, Schistocerca gregaria. Highly specific comprehensive 2-DE reference maps ("master gels") were developed for the brain, corpus cardiacum, subesophageal ganglion, and hemolymph. The polypeptides were well resolved within the pH 4-7 range in the first dimension and within the 14-94 kDa molecular mass range in the second dimension.
Annadurai
R S
RS
Max-Planck-Institut für Biochemie, Martinsried, Germany.
Oberthür
W
W
Rembold
H
H
D000818:Animals; D001923:Brain Chemistry; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D005724:Ganglia / Q000737:chemistry; D006110:Grasshoppers / Q000737:chemistry*; D006458:Hemolymph / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing; D008970:Molecular Weight; D009490:Neurosecretory Systems / Q000737:chemistry; D010449:Peptide Mapping