Initial organ localisation of blood-borne Candida albicans in a rat model of disseminated candidosis.
The rat was evaluated as an experimental model for disseminated candidosis by quantitating blood clearance and initial organ localisation of 3H-leucine-labelled Candida albicans after intravenous injection into the tail or portal vein. Viable or formalin-killed blastoconidia or viable blastoconidia with germ tubes were injected into experimental animals. Blood and tissue samples were obtained up to 24 h after injection and processed for liquid scintillation counting (to determine the distribution of labelled yeasts) and quantitation of viable organisms. Yeasts were cleared rapidly after intravenous (i.v.) injection by either route, i.e., < 5% of the radioactivity was detected in the blood after 5 min. The liver and lung were the major organs that sequestered blood-borne yeasts 1 h after tail vein injection (42.5 SD 15% and 41.4 SD 6.4% of labelled yeasts injected, respectively). However, injections via the portal vein resulted in trapping of the yeasts predominantly by the liver. Recovery of radioactivity and viable yeasts from all organs except the kidneys decreased with time. Overall, the results indicated that the rat might serve as a reliable model for short-term studies on organ distribution and thus contribute to our understanding of tissue trophism in candidosis.
Katz
S
S
Department of Surgery, James Whitcomb Riley Hospital for Children, Indianapolis, IN.
Grosfeld
J L
JL
Folkening
W J
WJ
Rosenthal
R S
RS
Merkel
G J
GJ
D000818:Animals; D001769:Blood / Q000382:microbiology*; D002177:Candidiasis / Q000209:etiology / Q000382:microbiology*; D004195:Disease Models, Animal; D005069:Evaluation Studies as Topic; D007275:Injections, Intravenous; D008099:Liver / Q000382:microbiology*; D008297:Male; D008657:Metabolic Clearance Rate; D009928:Organ Specificity; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors