2,4-Dichlorophenoxyacetic acid action on in vitro protein synthesis and its relation to polyamines.
The effect of 2,4-dichlorophenoxyacetic acid (2,4-D) on the in vitro synthesis of proteins was studied in Chinese hamster ovary cells. A remarkable inhibition of the synthesis of proteins was observed when cells grew for 24 h in presence of 1 mM 2,4-D. This effect was reversed by adding 0.1 mM of the three polyamines (putrescine, spermidine and spermine) to the cultured cells. The mRNA is not altered, indicating that the 2,4-D action is located at a different locus, which may be the ribosomes. From these studies, one can speculate that the alterations in the protein synthesis may be a consequence of the effect of 2,4-D on the polyamine metabolism.
Rivarola
V
V
Departamento Biologia Molecular, Facultad de Ciencias Exactas, Fisico-Quimicas y Naturales, Universidad Nacional de Rio Cuarto, Córdoba, Argentina.
Mori
G
G
Balegno
H
H
D015084:2,4-Dichlorophenoxyacetic Acid / Q000494:pharmacology*; D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D016466:CHO Cells / Q000187:drug effects / Q000378:metabolism; D006224:Cricetinae; D011073:Polyamines / Q000378:metabolism*; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism
D000596:Amino Acids; D011073:Polyamines; D012333:RNA, Messenger; D015084:2,4-Dichlorophenoxyacetic Acid