Combining in vivo volume-controlled pressure microejection with extracellular unit recording.
We report a method for combining extracellular single-unit recording with pressure ejection, permitting microvolume quantification through the measurement of meniscus movement. Good optimization of both high quality recording and precise determination (in the nanoliter range) of the pressure-ejected volume can be obtained by using a recording electrode affixed to a calibrated, narrow inner diameter ejection pipette.
Akaoka
H
H
INSERM U171 and CNRS URA 1195, Centre Hospitalier Lyon-Sud, Pierre-Bénite, France.
Saunier
C F
CF
Chergui
K
K
Charléty
P
P
Buda
M
M
Chouvet
G
G
D000818:Animals; D001921:Brain / Q000166:cytology; D004566:Electrodes; D005110:Extracellular Space / Q000502:physiology*; D008297:Male; D008845:Microinjections; D009474:Neurons / Q000502:physiology / Q000648:ultrastructure; D011312:Pressure; D051381:Rats; D017207:Rats, Sprague-Dawley