title: Distribution of ASFV antigens in pig tissues experimentally infected with two different Spanish virus isolates.
abstract: Lymph nodes, spleen, liver, lung and kidney obtained from pigs experimentally infected with two African Swine Fever Virus (ASFV) isolates of differing virulence were fixed by perfusion with glutaraldehyde and embedded in paraffin. An immunoperoxidase technique using a polyclonal anti-ASFV serum was performed on tissue sections in order to detect ASFV antigen. The distribution of ASFV antigen in such infected organs is shown and the differences between both infections compared and discussed. Monocytes, macrophages, hepatocytes, endothelial cells, neutrophils and epithelial cells were found to contain ASFV antigens.
authors.affiliation: Department of Histology and Pathological Anatomy, Veterinary Faculty, University of Córdoba, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Fernandez
authors.forename: J
authors.initials: J
authors.lastname: Perez
authors.forename: L
authors.initials: L
authors.lastname: Carrasco
authors.forename: M J
authors.initials: MJ
authors.lastname: Bautista
authors.forename: J M
authors.initials: JM
authors.lastname: Sanchez-Vizcaino
authors.forename: M A
authors.initials: MA
authors.lastname: Sierra
mesh_terms: D000357:African Swine Fever / Q000382:microbiology*; D000358:African Swine Fever Virus / Q000276:immunology*; D000818:Animals; D000956:Antigens, Viral / Q000032:analysis*; D007150:Immunohistochemistry; D008221:Lymphoid Tissue / Q000382:microbiology*; D008297:Male; D013552:Swine; D014781:Viscera / Q000382:microbiology*
chemical_list: D000956:Antigens, Viral