title: [The kinetics of ascorbic acid oxidation catalyzed by Cu(II)/H2DCA in the presence of DNA].
abstract: In phosphate buffer (pH 7.40), at 20.00 +/- 0.05 degrees C, the visible spectra of Cu(II) and Cu(II) complexes and the kinetics of ascorbic acid oxidation catalyzed by Cu(II)/H2DCA in the presence of DNA were studied. Measurements were reported on rate of .OH formation by the oxidation of ascorbic acid in the presence of Cu(II)/H2DCA. It showed that ternary complex Cu(II)/H2DCA/DNA may exist in the system in which DNA strand was broken by ascorbic acid in the presence of Cu(II)/H2DCA, and it was assumed that the DNA-breaking action of ascorbic acid in the presence of Cu(II)/H2DCA conformed to site-specific Fenton reaction mechanism. All the result of DNA-breaking action can be explained in terms of this site-specific Fenton reaction mechanism.
authors.affiliation: Physical Chemistry Department, School of Pharmaceutical Sciences, Beijing Medical University.
authors.forename: P H
authors.initials: PH
authors.lastname: Li
authors.forename: Q H
authors.initials: QH
authors.lastname: Chen
authors.forename: Y H
authors.initials: YH
authors.lastname: Pang
mesh_terms: D001205:Ascorbic Acid / Q000378:metabolism*; D003300:Copper / Q000494:pharmacology*; D004249:DNA Damage; D007700:Kinetics; D010084:Oxidation-Reduction
chemical_list: D003300:Copper; D001205:Ascorbic Acid