The differentiation of glial cell progenitor populations following transplantation into non-repairing central nervous system glial lesions in adult animals.
The non-repairing nature of the locally x-irradiated ethidium bromide (EB)-induced demyelinating white matter lesion has been further validated by showing that injections of two cultures which promote host remyelination of EB lesions in normal tissue do not do so in x-irradiated lesions. The behaviour of an oncogene-immortalized glial cell line and a growth-factor-expanded glial progenitor population have been examined following transplantation into the non-repairing EB lesion. Our studies indicate that the selected glial cell populations were each capable of establishing glial environments around demyelinated axons. Extensive oligodendrocyte remyelination with little astrocytic presence was observed in lesions transplanted with growth-factor-expanded optic nerve progenitors, while less extensive oligodendrocyte remyelination with the establishment of astrocyte-like cells was found in lesions transplanted with ts A58-SV40T immortalized glial cells. Prolonged expansion of both populations resulted in a loss of differentiation to normal glial phenotypes.
Crang
A J
AJ
Department of Clinical Veterinary Medicine, University of Cambridge, UK.
Franklin
R J
RJ
Blakemore
W F
WF
Noble
M
M
Barnett
S C
SC
Groves
A
A
Trotter
J
J
Schachner
M
M
D000818:Animals; D002454:Cell Differentiation; D002461:Cell Line, Transformed; D002493:Central Nervous System Diseases / Q000139:chemically induced / Q000473:pathology / Q000628:therapy*; D004996:Ethidium; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D009457:Neuroglia / Q000187:drug effects / Q000473:pathology / Q000637:transplantation*; D009857:Oncogenes; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology; D051381:Rats; D013116:Spinal Cord / Q000503:physiopathology / Q000528:radiation effects; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000187:drug effects / Q000473:pathology; D013696:Temperature; D014945:Wound Healing
D010982:Platelet-Derived Growth Factor; D016222:Fibroblast Growth Factor 2; D004996:Ethidium