Circulating and excreted forms of atrial natriuretic peptide in healthy subjects and patients with renal diseases.
The immunoreactivity of plasma and urine atrial natriuretic peptide (ANP) was measured in patients with renal disease and in healthy volunteers. The molecular forms of ANP in these subjects were estimated by gel permeation chromatography and reverse phase high performance liquid chromatography. No significant increase in plasma ANP was observed in patients with nephrotic syndrome or non-oliguric chronic renal failure compared to healthy volunteers. However, plasma ANP levels were significantly increased in patients on hemodialysis (normal 18.6 +/- 11.4 fmol/ml; hemodialysis 91.2 +/- 69.9 fmol/ml, p < 0.01). Chromatographic analyses revealed that plasma ANP consisted of only alpha-ANP or combined alpha- and gamma-ANP in healthy volunteers and in nephrotic patients, whereas beta-ANP frequently appeared in the plasma of both dialyzed and non-dialyzed chronic renal failure patients. Excreted forms, except in subjects free from renal disease where gamma-ANP may serve as a potential marker of glomerular injury in humans.
Marumo
F
F
Second Department of Internal Medicine, Tokyo Medical and Dental University, Japan.
Shichiri
M
M
Emori
T
T
Ando
K
K
D000328:Adult; D009320:Atrial Natriuretic Factor / Q000737:chemistry / Q000378:metabolism*; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006801:Humans; D007674:Kidney Diseases / Q000378:metabolism*; D007676:Kidney Failure, Chronic / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009404:Nephrotic Syndrome / Q000378:metabolism; D010446:Peptide Fragments / Q000378:metabolism; D011863:Radioimmunoassay; D006435:Renal Dialysis
D010446:Peptide Fragments; D009320:Atrial Natriuretic Factor