Transcriptional mapping of the promoter of the aminoglycoside acetyltransferase gene (aacC9) of neomycin-producing Micromonospora chalcea.
We have studied the promoter of the gene encoding aminoglycoside acetyltransferase (aacC9) in neomycin-producing Micromonospora chalcea. S1 nuclease mapping showed that the transcription initiation point of this gene is at the translation start point, with no evidence of a conventional ribosome-binding site. The aac of paromycin-producing Streptomyces rimosus forma paromomycinus shows the same characteristic; there is no homology in the promoter regions of the two genes, whereas the coding sequences are very similar.
Salauze
D
D
Département des Biotechnologies, Institut Pasteur, Paris.
Davies
J
J
D000123:Acetyltransferases / Q000235:genetics*; D002874:Chromosome Mapping; D066298:In Vitro Techniques; D008847:Micromonospora / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D009355:Neomycin / Q000096:biosynthesis*; D010303:Paromomycin / Q000096:biosynthesis; D011401:Promoter Regions, Genetic / Q000235:genetics*; D013302:Streptomyces / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic
D010303:Paromomycin; D000123:Acetyltransferases; C019681:aminoglycoside acetyltransferase; D009355:Neomycin