Localization of an adipocyte-specific retinoic acid response domain controlling S14 gene transcription.
S14 gene transcription is induced by retinoic acid (RA) in cultured adipocytes, but not preadipocytes. Transfection of 3T3-L1 cells with S14-CAT fusion genes showed that adipocyte-specific RA responsive cis-acting elements are located between -1588 and -1381 bp upstream from the 5' end of the S14 gene. This region has enhancer-like properties. In contrast, an artificial RA response element conferred RA control to a CAT gene in both preadipocyte and adipocyte phenotypes indicating that the RA regulatory network is functional in both phenotypes. These studies show that RA receptors interact with adipocyte-specific transcription factors to control S14 gene expression.
MacDougald
O A
OA
Department of Physiology, Michigan State University, East Lansing 48824.
Jump
D B
DB
D016475:3T3 Cells; D000273:Adipose Tissue / Q000187:drug effects / Q000502:physiology*; D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D015500:Chloramphenicol O-Acetyltransferase / Q000096:biosynthesis / Q000235:genetics; D004790:Enzyme Induction; D051379:Mice; D008969:Molecular Sequence Data; D009687:Nuclear Proteins; D009838:Oligodeoxyribonucleotides; D011401:Promoter Regions, Genetic; D011506:Proteins / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D015183:Restriction Mapping; D013937:Thymidine Kinase / Q000235:genetics; D014157:Transcription Factors; D014158:Transcription, Genetic / Q000187:drug effects*; D014162:Transfection; D014212:Tretinoin / Q000494:pharmacology*
D009687:Nuclear Proteins; D009838:Oligodeoxyribonucleotides; D011506:Proteins; D011993:Recombinant Fusion Proteins; C051065:THRSP protein, human; C501477:Thrsp protein, mouse; D014157:Transcription Factors; D014212:Tretinoin; D015500:Chloramphenicol O-Acetyltransferase; D013937:Thymidine Kinase