Identification of C-terminal peptides of bovine beta-casein that enhance proliferation of rat lymphocytes.
A casein polypeptidic fraction, obtained from a pepsin-chymosin digestion of caseins, showed a mitogenic effect on primed lymph node (LN) cells and unprimed spleen cells of rats. A biologically active C-terminal sequence of bovine beta-casein (residues 192-209) was characterized. The corresponding synthetic peptide had a stimulatory effect on primed LN cells but failed to enhance proliferation of spleen cells. We prepared two chymosin digests (PA and PB) of bovine beta-casein which contained, respectively, 80% and 95% of the sequence including residues 193-209. They induced a significant proliferative response in both LN and spleen cells. It is therefore possible that other active peptides in the PA preparation may be involved in mitogenic activity.
Coste
M
M
Unité de Nutrition Humaine et Physiologie Intestinale, INRA, Faculté des Science Pharmaceutiques et Biologiques, Paris, France.
Rochet
V
V
Léonil
J
J
Mollé
D
D
Bouhallab
S
S
Tomé
D
D
D000595:Amino Acid Sequence; D000818:Animals; D002364:Caseins / Q000378:metabolism / Q000494:pharmacology*; D002417:Cattle; D002455:Cell Division / Q000187:drug effects; D002918:Chymotrypsin / Q000378:metabolism; D005260:Female; D008213:Lymphocyte Activation / Q000187:drug effects; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects*; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000494:pharmacology*; D011492:Protein Hydrolysates / Q000494:pharmacology; D051381:Rats; D011914:Rats, Inbred BN / Q000276:immunology; D017208:Rats, Wistar / Q000276:immunology
D002364:Caseins; D010446:Peptide Fragments; D011492:Protein Hydrolysates; D002918:Chymotrypsin