Nucleobase transporter-mediated permeation of 2',3'-dideoxyguanosine in human erythrocytes and human T-lymphoblastoid CCRF-CEM cells.
Several 2',3'-dideoxynucleosides (ddNs), agents that inhibit the replication of human immunodeficiency virus and hepatitis B virus, enter mammalian cells by simple diffusion. In this report, we show that the membrane permeation of 2',3'-dideoxyguanosine (ddG) in human erythrocytes and CCRF-CEM cells, in contrast with that of other ddNs, is transporter-mediated. Inward fluxes of ddG in both cell types were inhibited by adenine, hypoxanthine, and acyclovir, but not by inhibitors of nucleoside transport (nitrobenzylthioinosine, dipyridamole, dilazep). Fluxes of ddG in human erythrocytes were attributable to a single, rate-saturable process (Km, 380 +/- 90 microM and Vmax, 7.9 +/- 0.8 pmol/s/microliter cell water) that was competitively inhibited by adenine (Ki, 16 microM). These results showed that ddG entered human erythrocytes and CCRF-CEM cells by a transporter-mediated process that was also the basis for entry of purine nucleobases. In contrast, inward fluxes of 2,6-diaminopurine-2',3'-dideoxyriboside (ddDAPR), a prodrug of ddG, were not affected by purine nucleobases or nucleoside transport inhibitors in either cell type. Thus, the permeation properties of ddDAPR resembled those of 2',3'-dideoxyadenosine, a diffusional permeant (cell uptake is transporter-independent), and contrasted with those of ddG, the deamination product of ddDAPR. This study demonstrated that the nucleobase moiety of ddNs is an important determinant of membrane permeation.
Gati
W P
WP
Department of Pharmacology, University of Alberta, Edmonton, Canada.
Paterson
A R
AR
Tyrrell
D L
DL
Cass
C E
CE
Moravek
J
J
Robins
M J
MJ
D001692:Biological Transport; D002352:Carrier Proteins; D002462:Cell Membrane / Q000378:metabolism; D015224:Dideoxynucleosides / Q000378:metabolism*; D004912:Erythrocytes / Q000378:metabolism*; D006151:Guanosine; D006801:Humans; D066298:In Vitro Techniques; D013601:T-Lymphocytes / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D002352:Carrier Proteins; D015224:Dideoxynucleosides; D006151:Guanosine; C050220:2',3'-dideoxyguanosine