Dot-ELISA as a diagnostic test in hydatid disease.
Detection of circulating echinococcal antigens and antibodies were tried in 18 patients with hydatid disease and 32 healthy blood donors as controls using dot-ELISA. Hydatid cyst fluid antigen and anti-echinococcal hyperimmune rabbit sera were used for the detection of antibodies and antigens respectively. The results proved that this test is sensitive (88.9%), specific (96.9%) and rapid for detecting echinococcal antibodies. On the other hand, circulating antigens could be detected with 55.6% sensitivity. This low sensitivity was attributed to the small amounts of circulating antigens and/or immune complexes formation.
Romia
S A
SA
Department of Parasitology, General Medicine, Faculty of Medicine, Mansoura University, Egypt.
Youssef
M E
ME
Handoussa
A E
AE
Rizk
H M
HM
Sallam
S M
SM
D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D000947:Antigens, Helminth / Q000097:blood*; D004443:Echinococcosis / Q000175:diagnosis*; D004446:Echinococcus / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D012680:Sensitivity and Specificity
D000909:Antibodies, Helminth; D000947:Antigens, Helminth