title: Structure of the tilapia (Oreochromis mossambicus) prolactin I gene.
abstract: The tilapia (Oreochromis mossambicus) prolactin-I (PRL-I) gene has been cloned and sequenced. Its transcript (3,677 bases long) begins with a guanine and is organized in five exons and four introns like the other known prolactin genes. Analysis of the 1,555-bp 5'-flanking region suggests that pituitary-specific expression of the gene could be regulated through a trans-factor related to the mammalian pituitary-specific factor Pit-1. Two potential binding sites for such a factor were found in the first intron, suggesting a possible regulatory role for this region. Moreover, two potential Z-DNA regions are located at positions -837 to -812 and -246 to -179 from the transcription start site. These two regions could play an important role in the regulation of PRL gene expression.
authors.affiliation: Laboratoire de Biologie Moléculaire et de Génie Génétique, Université de Liège, Sart Tilman, Belgium.
authors.forename: D
authors.initials: D
authors.lastname: Swennen
authors.forename: A C
authors.initials: AC
authors.lastname: Poncelet
authors.forename: B
authors.initials: B
authors.lastname: Sekkali
authors.forename: F
authors.initials: F
authors.lastname: Rentier-Delrue
authors.forename: J A
authors.initials: JA
authors.lastname: Martial
authors.forename: A
authors.initials: A
authors.lastname: Belayew
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004247:DNA; D006801:Humans; D008969:Molecular Sequence Data; D011388:Prolactin / Q000235:genetics*; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D017210:Tilapia; D014158:Transcription, Genetic
chemical_list: D011388:Prolactin; D004247:DNA