Ring test for low levels of N-(2-hydroxyethyl)valine in human hemoglobin.
Hemoglobin adducts are useful for the identification and quantification of electrophilic agents in vivo. A modified Edman degradation method has been extensively used for monitoring exposure to ethylene oxide through gas chromatographic-mass spectrometric measurements of hydroxyethyl adducts to the N-terminal valines in hemoglobin. In a ring test, four laboratories using different versions of the method analyzed eight human globin samples with low adduct levels from ethylene oxide. Measurements of the same adduct by a radioimmunoassay were also included. Strong correlation between the measurements by the different laboratories shows that the method in principle works well. However, there were some systematic quantitative differences.
Törnqvist
M
M
Department of Radiobiology, Stockholm University, Sweden.
Magnusson
A L
AL
Farmer
P B
PB
Tang
Y S
YS
Jeffrey
A M
AM
Wazneh
L
L
Beulink
G D
GD
van der Waal
H
H
van Sittert
N J
NJ
D006454:Hemoglobins / Q000737:chemistry*; D006801:Humans; D011863:Radioimmunoassay; D012044:Regression Analysis; D014633:Valine / Q000031:analogs & derivatives* / Q000032:analysis
D006454:Hemoglobins; C057442:2-hydroxyethylvaline; D014633:Valine