title: Unusual manifestations of Yersinia enterocolitica infections diagnosed using novel methods.
abstract: We report the cases of two patients who had infections due to Yersinia enterocolitica. The first patient exhibited chronic recurrent fever, hepatic and splenic granulomas, and bone marrow abnormalities, and the second patient presented with enterocolitis with leukocytoclastic vasculitis of the skin. Cultures and agglutination titers were negative. Indirect immunofluorescence techniques with use of serotype-specific antisera and antisera to Yersinia outer-membrane proteins (Yops) were applied to biopsy specimens, and immunoblotting techniques for determining class-specific circulating antibodies to Yops were used for demonstrating these unusual manifestations of Y. enterocolitica infections.
authors.affiliation: Department of Pathology, University of Leiden, The Netherlands.
authors.forename: P P
authors.initials: PP
authors.lastname: Tak
authors.forename: L G
authors.initials: LG
authors.lastname: Visser
authors.forename: J A
authors.initials: JA
authors.lastname: Hoogkamp-Korstanje
authors.forename: J C
authors.initials: JC
authors.lastname: Kluin-Nelemans
authors.forename: P C
authors.initials: PC
authors.lastname: Hogendoorn
authors.forename: P M
authors.initials: PM
authors.lastname: Kluin
authors.forename: M
authors.initials: M
authors.lastname: Barza
authors.forename: J
authors.initials: J
authors.lastname: de Koning
authors.forename: R
authors.initials: R
authors.lastname: van Furth
mesh_terms: D000328:Adult; D000368:Aged; D000907:Antibodies, Bacterial / Q000032:analysis; D016470:Bacteremia / Q000175:diagnosis / Q000382:microbiology; D004760:Enterocolitis / Q000382:microbiology; D005260:Female; D005455:Fluorescent Antibody Technique; D006099:Granuloma / Q000175:diagnosis / Q000382:microbiology; D006801:Humans; D015151:Immunoblotting; D008107:Liver Diseases / Q000175:diagnosis* / Q000382:microbiology; D017192:Skin Diseases, Bacterial / Q000175:diagnosis* / Q000382:microbiology; D013158:Splenic Diseases / Q000175:diagnosis* / Q000382:microbiology; D015009:Yersinia Infections / Q000175:diagnosis* / Q000382:microbiology; D015008:Yersinia enterocolitica / Q000302:isolation & purification*
chemical_list: D000907:Antibodies, Bacterial