title: A radiation hybrid map of the proximal short arm of the human X chromosome spanning incontinentia pigmenti 1 (IP1) translocation breakpoints.
abstract: Radiation hybrid mapping was used in combination with physical mapping techniques to order and estimate distances between 14 loci in the proximal region of the short arm of the human X chromosome. A panel of radiation hybrids containing human X-chromosomal fragments was generated from a Chinese hamster-human cell hybrid containing an X chromosome as its only human DNA. Sixty-seven radiation hybrids were screened by Southern hybridization with sets of probes that mapped to the region Xp11.4-Xcen to generate a radiation hybrid map of the area. A physical map of 14 loci was constructed based on the segregation of the loci in the hybrid clones. Using pulsed-field gel electrophoresis (PFGE) analyses and a somatic cell hybrid mapping panel containing naturally occurring X; autosome translocations, the order of the 14 loci was verified and the loci nearest to the X-chromosomal translocation breakpoints associated with the disease incontinentia pigmenti 1 (IP1) were identified. The radiation hybrid panel will be useful as a mapping resource for determining the location, order, and distances between other genes and polymorphic loci in this region as well as for generating additional region-specific DNA markers.
authors.affiliation: Department of Pediatrics and Communicable Diseases, University of Michigan, Ann Arbor 48109-0688.
authors.forename: J L
authors.initials: JL
authors.lastname: Gorski
authors.forename: M
authors.initials: M
authors.lastname: Boehnke
authors.forename: E L
authors.initials: EL
authors.lastname: Reyner
authors.forename: E N
authors.initials: EN
authors.lastname: Burright
mesh_terms: D000818:Animals; D015139:Blotting, Southern; D002874:Chromosome Mapping; D006224:Cricetinae; D016521:Electrophoresis, Gel, Pulsed-Field; D008040:Genetic Linkage; D006801:Humans; D006822:Hybrid Cells; D007184:Incontinentia Pigmenti / Q000235:genetics*; D014178:Translocation, Genetic; D014960:X Chromosome / Q000528:radiation effects