Effect of buparvaquone on the expression of interleukin 2 receptors in Theileria annulata-infected cells.
Theileria annulata-infected cells were cultured in the presence or absence of human recombinant interleukin 2 (hrIL-2). This growth factor proved to be capable of enhancing the growth of the infected cells: its effect was marked, particularly when the cells were seeded at low densities, and it varied from cell line to cell line. The infected cells produced a factor that possessed the biological activities of IL-2, since their supernatants could enhance the proliferation of concanvalin A-stimulated (Con A) blasts. The reactivity of the parasitized cells to hrIL-2 was abolished following their treatment with the antitheilerial drug buparvaquone. In addition, the drug inhibited the binding of 125I-IL-2 to T. annulata-infected cells but failed to suppress its binding to Con A blasts. Northern blot analysis revealed that the drug had no effect on the expression of the alpha chain of the IL-2 receptor (IL-2R). Therefore, it is possible that buparvaquone interferes with the expression of the beta chain of the IL-2R. The role of IL-2 and the IL2R in the permanent proliferation of T. annulata-infected cells is discussed.
Ahmed
J S
JS
Institut für Parasitologie und Tropenveterinärmedizin, Freie Universität Berlin, Federal Republic of Germany.
Rintelen
M
M
Schein
E
E
Williams
R O
RO
Dobbelaere
D
D
D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D005786:Gene Expression Regulation; D006801:Humans; D007376:Interleukin-2 / Q000502:physiology*; D008213:Lymphocyte Activation / Q000187:drug effects; D008214:Lymphocytes / Q000187:drug effects / Q000469:parasitology*; D009285:Naphthoquinones / Q000494:pharmacology*; D012333:RNA, Messenger / Q000096:biosynthesis; D015375:Receptors, Interleukin-2 / Q000096:biosynthesis / Q000187:drug effects* / Q000235:genetics; D011994:Recombinant Proteins / Q000378:metabolism; D016796:Theileria annulata / Q000502:physiology*
D007376:Interleukin-2; D009285:Naphthoquinones; D012333:RNA, Messenger; D015375:Receptors, Interleukin-2; D011994:Recombinant Proteins; C046326:buparvaquone