Evaluation of the Pro-Lab ID ring system for the identification of medically important yeasts.
We evaluated 151 coded isolates of medically important yeast species belonging to the genera Candida, Cryptococcus, Geotrichum, Rhodoturula, Saccharomyces and Torulopsis using the newly developed rapid Pro-Lab Identification Ring, PL 960 system (PLID-Ring). All isolates were concurrently identified by the API 20C and conventional procedures comprising macro- and micromorphology, assimilation and fermentation of various carbon and nitrogen compounds. The PLID-Ring system identified isolates of Candida albicans, C. kefyr, C. krusei, C. lusitaniae, C. parapsilosis, Rhodotorula rubra, and Torulopsis glabrata with 100% accuracy in 24 h. This system identified C guilliermondii and S. cerevisiae isolates with an accuracy of 90% and 86%, respectively, while those belonging to Cr. neoformans, T. candida (= C. famata), C. rugosa and C. tropicalis were identified with 38.4%, 50%, 12.5% and 50% accuracy, respectively. Three isolates of Cr. laurentii were not identified by the PLID-Ring system. The overall accuracy of the PLID-Ring system was 81.45% (123 of 151 isolates). However, the system does not include species such as Cr. laurentii in its data base. When these three Cr. laurentii isolates were excluded from the evaluation, the accuracy of the PLID-Ring system increased from 81.45% to 83.1%.
Sekhon
A S
AS
National Reference Centre for Human Mycotic Diseases, Provincial Laboratory of Public Health, University of Alberta, Edmonton, Canada.
Padhye
A A
AA
Garg
A K
AK
Rae
R
R
D002175:Candida / Q000302:isolation & purification; D003454:Cryptococcus / Q000302:isolation & purification; D005848:Geotrichum / Q000302:isolation & purification; D009181:Mycoses / Q000382:microbiology*; D011933:Reagent Kits, Diagnostic; D012248:Rhodotorula / Q000302:isolation & purification; D012440:Saccharomyces / Q000302:isolation & purification; D015003:Yeasts / Q000254:growth & development / Q000302:isolation & purification*
D011933:Reagent Kits, Diagnostic