Application of an automated plasma filtration device and a blood monitoring system for LDL apheresis.
This study describes the adaptation of a filtration separation device (AK 10 from Gambro, München, FRG) and a blood monitor system (BTS 100 from Diamed, Köln, FRG) to the automated adsorption/desorption device for LDL apheresis. Plasma separation by filtration was performed by a hollow-fiber filter (OP5, Asahi Corp., Japan). In order to evaluate the quality of the plasma obtained by filtration, the platelet contamination, the activity of factors V and VIII and the concentration of the immunoglobulins IgG and IgM were determined. The following results were found: Platelet contamination of the plasma was 2,740/microliters. As compared to the patient's pretreatment values, activity of factor V was 94%, activity of factor VIII was 104%, concentration of IgG was 96%, concentration of IgM was 94%. Ten LDL aphereses were evaluated: red cell counts, platelet counts, hemoglobin concentration and hematocrit were not changed significantly. Thus, this new combination system represents an improved alternative to previously performed centrifugation methods.
Taborski
U
U
Department of Hemostaseology and Transfusion Medicine, Kerckhoff-Klinik, Bad Nauheim.
Köhler
T
T
Schüpphaus
S
S
Hofmann
M R
MR
Müller-Berghaus
G
G
D001772:Blood Cell Count; D001781:Blood Component Removal / Q000295:instrumentation*; D008078:Cholesterol, LDL / Q000097:blood*; D005165:Factor V / Q000032:analysis; D005169:Factor VIII / Q000032:analysis; D006400:Hematocrit; D006440:Hemofiltration / Q000295:instrumentation*; D006452:Hemoglobinometry; D006801:Humans; D006937:Hypercholesterolemia / Q000097:blood / Q000628:therapy*; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D007163:Immunosorbent Techniques / Q000295:instrumentation*
D008078:Cholesterol, LDL; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D005165:Factor V; D005169:Factor VIII