title: Characterisation of the expression and post-translational processing of the preprotachykinin-I gene and the regulated release of tachykinins by the RINm5F cell-line.
abstract: Normal transcription and postranslational processing of the preprotachykinin (PPT)-I gene and regulated release of substance P and neurokinin A by the rat pancreatic endocrine cell-line, RINm5F, has been demonstrated, using radioimmunoassays (RIAs), reversed-phase (rp)HPLC and Northern blot analysis. This is the first stable cell-line found to express the PPT-I gene and provides an opportunity for investigating PPT-I gene expression and tachykinin biosynthesis. RIN5mF cells are a model for the pancreatic beta-cell, which is not known to exhibit PPT-I gene expression which may, therefore, be a feature of the transformed state of these cells. These data may imply that the tachykinins are important in pancreatic islet embryogenesis.
authors.affiliation: Department of Physiology, University of Marburg, Germany.
authors.forename: G P
authors.initials: GP
authors.lastname: McGregor
authors.forename: R
authors.initials: R
authors.lastname: Hartel
authors.forename: H C
authors.initials: HC
authors.lastname: Fehmann
authors.forename: B
authors.initials: B
authors.lastname: Lankat-Buttgereit
authors.forename: B
authors.initials: B
authors.lastname: Göke
authors.forename: R
authors.initials: R
authors.lastname: Göke
authors.forename: K
authors.initials: K
authors.lastname: Voigt
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D002851:Chromatography, High Pressure Liquid; D007340:Insulinoma; D011498:Protein Precursors / Q000235:genetics* / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D051381:Rats; D015320:Tachykinins / Q000235:genetics* / Q000378:metabolism*; D014407:Tumor Cells, Cultured
chemical_list: D011498:Protein Precursors; D015320:Tachykinins; C044206:preprotachykinin