Simian immunodeficiency virus vectors: replication and pseudotyping.
We studied a single round of replication of Simian immunodeficiency virus (SIV) through the use of a replication defective vector that expresses the hygromycin resistance gene. It was possible to pseudotype SIV particles by complementation with the env gene from a murine amphotropic retrovirus. Moreover, SIV RNA was packaged and propagated by core particles of the heterologous lentivirus, HIV-1. These results indicate that coinfection of cells with SIV and other retroviruses could lead to infection of new cell types in nature.
Rizvi
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McArdle Laboratory for Cancer Research, University of Wisconsin-Madison 53706.
Panganiban
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D000818:Animals; D015139:Blotting, Southern; D002460:Cell Line; D002934:Cinnamates; D004279:DNA, Viral / Q000032:analysis; D004352:Drug Resistance, Microbial / Q000235:genetics; D015967:Gene Expression Regulation, Viral; D015752:Genes, env; D005822:Genetic Vectors; D006367:HeLa Cells; D006801:Humans; D006921:Hygromycin B / Q000031:analogs & derivatives / Q000494:pharmacology; D009693:Nucleic Acid Hybridization; D011533:Proviruses / Q000235:genetics / Q000502:physiology; D015302:Simian Immunodeficiency Virus / Q000145:classification / Q000187:drug effects / Q000235:genetics* / Q000502:physiology; D014162:Transfection; D014779:Virus Replication
D002934:Cinnamates; D004279:DNA, Viral; D006921:Hygromycin B; C026273:hygromycin A