Time-resolved x-ray diffraction study of the troponin-associated reflexions from the frog muscle.
The vertebrate skeletal muscle gives rise to a series of x-ray reflexions indexed as orders (n) of 77 nm, the even orders being meridional whereas the odd orders being near-meridional. The diffraction intensities associated with these reflexions originate from the axial period of 39 nm attributable to the repeat of troponin-tropomyosin on the thin filament. In the present study, the x-ray intensities of the furthest inner reflexions, A2 (n = 2) reflexion at an axial spacing of 1/39 nm-1 and A4 (n = 4) reflexion at 1/19 nm, of this series were measured with a time resolved manner. Upon activation of the frog striated muscle, the two reflexions underwent biphasic time courses of the intensity changes. With A2 reflexion, a rapid intensity increase by 16%, being completed by the time when tension rises to 5%, was followed by a slow intensity decrease down to 50%, which was associated with the tension rise. In both phases, lateral widths remained unchanged. A4 reflexion also behaves in the same way, although the first phase (the intensity increase) was not clear due to unsatisfactory statistics. We interpret phase one as being caused by conformational change of the troponin-tropomyosin complex upon binding of Ca2+ to troponin, whereas phase two being due to direct contribution of the mass of the myosin heads bound to the thin filament, although possible contribution of conformational changes of the regulatory proteins to phase two is not excluded. The results indicated that the calcium activation of the thin filament leads the onset of the actomyosin interaction.
Maéda
Y
Y
European Molecular Biology Laboratory, DESY, Hamburg, Germany.
Popp
D
D
Stewart
A A
AA
D000818:Animals; D007700:Kinetics; D009132:Muscles / Q000502:physiology* / Q000648:ultrastructure; D011894:Rana pipiens; D013816:Thermodynamics; D013997:Time Factors; D014336:Troponin / Q000502:physiology* / Q000648:ultrastructure*; D014961:X-Ray Diffraction / Q000379:methods
D014336:Troponin