Formation of antigenic extracellular polysaccharides by selected strains of Mucor spp., Rhizopus spp., Rhizomucor spp., Absidia corymbifera and Syncephalastrum racemosum.
In this study, polyclonal IgG antibodies raised against extracellular polysaccharides (EPS) of Mucor racemosus were characterised as almost specific for moulds belonging to the order of Mucorales. Cross-reactivity in the ELISA could be observed only towards the yeast Pichia membranaefaciens. EPS were isolated from various cultures of M. hiemalis growing on six different carbon sources and two nitrogen sources, with ratios varying from 0.13 to 0.44 relative to the amount of biomass. Other strains including Mucor spp., Rhizopus spp., Rhizomucor spp., Absidia corymbifera and Syncephalastrum racemosum also excreted EPS, with ratios varying from 0.05 to 0.23. In all cases, the excreted EPS had similar antigenic properties as determined by ELISA. No enzymatic degradation of the antigenic parts of the polysaccharides could be observed upon prolonged incubation. Considering that all tested strains formed similar amounts of antigenic EPS there might be scope for the specific detection of biomass of Mucoralean moulds using ELISA techniques for example in food.
De Ruiter
G A
GA
Wageningen Agricultural University, Department of Food Science, The Netherlands.
Van Bruggen-van der Lugt
A W
AW
Nout
M J
MJ
Middelhoven
W J
WJ
Soentoro
P S
PS
Notermans
S H
SH
Rombouts
F M
FM
D000908:Antibodies, Fungal / Q000276:immunology; D000918:Antibody Specificity; D000946:Antigens, Fungal / Q000096:biosynthesis* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005947:Glucose / Q000378:metabolism; D007074:Immunoglobulin G / Q000276:immunology; D009089:Mucor / Q000254:growth & development / Q000276:immunology / Q000378:metabolism; D009090:Mucorales / Q000254:growth & development / Q000276:immunology* / Q000378:metabolism; D011134:Polysaccharides / Q000096:biosynthesis / Q000276:immunology*; D012233:Rhizopus / Q000254:growth & development / Q000276:immunology / Q000378:metabolism
D000908:Antibodies, Fungal; D000946:Antigens, Fungal; D007074:Immunoglobulin G; D011134:Polysaccharides; D005947:Glucose