title: The effects of histamine administered in fish samples to healthy volunteers.
abstract: The effects of histamine administered in samples of fish to eight healthy volunteers (4 females and 4 males), aged 21-30 years, were studied. The subjects were given 0, 45 and 90 mg of histamine that had been metabolized from histidine by photobacteria in the fish and 90 mg of histamine added to fresh fish, for breakfast. The subjects were observed during 6 h after breakfast. Special attention was paid to clinical symptoms, blood pressure and ECG. The pH of the gastric contents was recorded continuously from 5 min before to 6 h after the meal. Blood samples to measure the histamine concentration were taken at intervals during 24 h after breakfast. Two of the subjects showed effects (facial flushing, headache) that could be attributed to the ingestion of histamine. No significant changes were observed in the blood pressure and ECG. The pH of the gastric fluids did not decrease significantly. The histamine concentration in plasma correlated closely with the histamine dose ingested (p < 0.001, r = 0.996). The Cmax of the dose of 90 mg did not differ statistically significant from the Cmax of the dose of 90 mg histamine added to unspoiled fish.
authors.affiliation: National Poison Control Centre, National Institute of Public Health and Environmental Protection, Bilthoven, The Netherlands.
authors.forename: C E
authors.initials: CE
authors.lastname: van Gelderen
authors.forename: T J
authors.initials: TJ
authors.lastname: Savelkoul
authors.forename: L A
authors.initials: LA
authors.lastname: van Ginkel
authors.forename: W
authors.initials: W
authors.lastname: van Dokkum
mesh_terms: D000328:Adult; D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D004311:Double-Blind Method; D004562:Electrocardiography; D005260:Female; D005399:Fishes; D005483:Flushing / Q000139:chemically induced; D005506:Food Contamination; D005750:Gastric Juice / Q000737:chemistry; D006261:Headache / Q000139:chemically induced; D006321:Heart / Q000187:drug effects; D006632:Histamine / Q000009:adverse effects* / Q000097:blood; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008297:Male; D011897:Random Allocation
chemical_list: D006632:Histamine