title: Solid-phase extraction and HPLC determination of 4-vinyl guaiacol and its precursor, ferulic acid, in orange juice.
abstract: This study is undertaken to develop a simplified, rapid method to determine both immediate and potential off odors due to 4-vinyl guaiacol and its odorless precursor, ferulic acid, from a single sample preparation and chromatographic analysis. Orange juice sample preparation consists of a simple, C18 solid phase extraction. Utilizing a 5-microns, 25-cm, C18 column, both compounds can be separated within 40 min using a one-step, linear gradient beginning with an aqueous 12% tetrahydrofuran (THF)-5% acetonitrile mixture and ending with 35% aqueous THF. Hesperidin and nariutin have been identified as the compounds that interfered with the ultraviolet (UV) determination of sinapic and caffeic acids. Fluorescence detection with wavelength programming offers optimal sensitivity and selectivity. Recoveries of 4-vinyl guaiacol and ferulic acid range from 90 to 103%. Detection limits are 1 ppm and 5 ppm for ferulic acid and 4-vinyl guaiacol, respectively. Other hydroxycinnamic acids such as coumaric, sinapic, and caffeic acids may also be determined from the same chromatogram.
authors.affiliation: University of Florida, Institute of Food and Agricultural Sciences, Lake Alfred 33850.
authors.forename: R L
authors.initials: RL
authors.lastname: Rouseff
authors.forename: G R
authors.initials: GR
authors.lastname: Dettweiler
authors.forename: R M
authors.initials: RM
authors.lastname: Swaine
authors.forename: M
authors.initials: M
authors.lastname: Naim
authors.forename: U
authors.initials: U
authors.lastname: Zehavi
mesh_terms: D001628:Beverages / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D002957:Citrus / Q000737:chemistry*; D003373:Coumaric Acids / Q000032:analysis*; D006139:Guaiacol / Q000031:analogs & derivatives* / Q000032:analysis; D007202:Indicators and Reagents; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet
chemical_list: D003373:Coumaric Acids; D007202:Indicators and Reagents; D006139:Guaiacol; C014245:4-vinylguaiacol; C004999:ferulic acid