The effect of histamine on cultured endothelial cells. A study of the mechanism of increased vascular permeability.
The role of endothelial cells in the histamine-induced vascular response was investigated using cultured endothelial cells obtained from human umbilical veins. A single population of histamine H1 receptors was detected in these cells by means of a [3H]mepyramine binding assay. Its Kd was 0.74 +/- 0.07 nM and Bmax was 41.4 +/- 8.68 fmol/mg protein. Actin filaments were distributed as dense bands at the margin of the cells and as sparse microfilament bundles traversing the center of the cells. Histamine caused a decrease in peripheral bands and an increase in longitudinal bands. The changes evoked by histamine were dose-dependent, related to the duration of incubation of the cells with histamine, and blocked by mepyramine, a histamine H1 receptor antagonist. The results suggest that the endothelial cells respond to histamine through the histamine H1 receptor. This may explain one of the mechanisms of histamine-induced vascular response including vascular permeability increase.
Niimi
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N
Department of Dermatology, Hiroshima University, School of Medicine, Japan.
Noso
N
N
Yamamoto
S
S
D000199:Actins / Q000032:analysis; D002199:Capillary Permeability / Q000187:drug effects*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D006632:Histamine / Q000494:pharmacology*; D006801:Humans; D011738:Pyrilamine / Q000378:metabolism / Q000494:pharmacology
D000199:Actins; D006632:Histamine; D011738:Pyrilamine