title: Deposition and imaging of metal-coated biomolecules with the STM.
abstract: We have applied a simple and reliable procedure for imaging biomolecules with the scanning tunneling microscope (STM). The biomolecules are adsorbed on glow-discharged mica, then coated with a thin film of platinum-carbon. We have tested this method with linear and circular (plasmid) DNA molecules. The contrast and resolution of the STM images are comparable to electron micrographs of the same molecules when shadowed. Though the present lateral resolution (5-6 nm) is limited by the grain size of the conductive film, some details like supercoiled regions in the DNA are resolved. This method is interesting for two reasons. First, as an alternative technique for imaging biomolecules. Second, for use as a control in STM studies of bare biomolecules.
authors.affiliation: Institute of Molecular Biology, University of Oregon, Eugene 97403.
authors.forename: R
authors.initials: R
authors.lastname: García
authors.forename: J
authors.initials: J
authors.lastname: Yuqiu
authors.forename: E
authors.initials: E
authors.lastname: Schabtach
authors.forename: C
authors.initials: C
authors.lastname: Bustamante
mesh_terms: D004247:DNA / Q000648:ultrastructure*; D004270:DNA, Circular / Q000648:ultrastructure; D004279:DNA, Viral / Q000648:ultrastructure; D008670:Metals; D016252:Microscopy, Scanning Tunneling / Q000379:methods*; D010957:Plasmids / Q000648:ultrastructure; D013604:T-Phages / Q000648:ultrastructure
chemical_list: D004270:DNA, Circular; D004279:DNA, Viral; D008670:Metals; D004247:DNA