title: Differences in arachidonic acid metabolism by human myelomonocytic cell lines.
abstract: The production of arachidonic acid metabolites by the HL60, ML3, and U937 human phagocyte cell lines was determined after incubation with interferon-gamma (IFN-gamma, 500 U/ml) or vehicle for 4 days. Cells were prelabeled with tritiated arachidonic acid, [3H]AA, for 4 h, and media supernatants were analyzed by high-performance liquid chromatography. None of the cell lines produced [3H]AA metabolites in large amounts during an unstimulated, basal release period (30 or 60 min). In response to 10 microM calcium ionophore A23187 incubation (30 min), undifferentiated and IFN-gamma-differentiated HL60 cells formed both cyclooxygenase products (thromboxane and prostaglandins) and lipoxygenase products (leukotrienes and hydroxyeicosatetraenoic acids). In contrast to the HL60 cells, IFN-gamma-differentiated U937 cells formed primarily cyclooxygenase products and undifferentiated and IFN-gamma-differentiated ML3 cells did not form any [3H]AA metabolites in response to A23187. These results indicate the need to be careful in selecting a cell line for use in a phagocyte assay system when cyclooxygenase and/or lipoxygenase products could influence the assay results.
authors.affiliation: Center for Environmental Medicine and Lung Biology, University of North Carolina, Chapel Hill 27599.
authors.forename: M C
authors.initials: MC
authors.lastname: Madden
authors.forename: S
authors.initials: S
authors.lastname: Becker
authors.forename: H S
authors.initials: HS
authors.lastname: Koren
authors.forename: M
authors.initials: M
authors.lastname: Friedman
mesh_terms: D015703:Antigens, CD / Q000378:metabolism; D016718:Arachidonic Acid / Q000378:metabolism*; D002454:Cell Differentiation / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D015777:Eicosanoids / Q000378:metabolism; D006684:HLA-DR Antigens / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D007371:Interferon-gamma / Q000494:pharmacology; D015289:Leukotrienes / Q000378:metabolism; D009000:Monocytes / Q000166:cytology; D010586:Phagocytes / Q000166:cytology*; D011961:Receptors, Fc / Q000378:metabolism; D014407:Tumor Cells, Cultured / Q000378:metabolism*
chemical_list: D015703:Antigens, CD; D015777:Eicosanoids; D006684:HLA-DR Antigens; D015289:Leukotrienes; D011961:Receptors, Fc; D016718:Arachidonic Acid; D007371:Interferon-gamma