title: Human cholecystitis is associated with increased gallbladder prostaglandin I2 and prostaglandin E2 synthesis.
abstract: Microsomal prostanoid synthesis was compared in normal gallbladders removed during organ donation and inflamed gallbladders removed at cholecystectomy. Normal human gallbladder microsomes demonstrated low rates of conversion of [14C]arachidonic acid to total labeled prostanoids, which increased during 1 to 30 min of incubation. Normal human gallbladder microsomes converted labeled substrate to all primary prostaglandins without demonstration of a major product. Inflamed human gallbladder microsomes increased the rate of conversion of [14C]arachidonic acid to total labeled prostanoids two or three times over the levels demonstrated by normal gallbladder microsomes at all times of incubation (p < 0.01). The main prostanoids synthesized by the inflamed human gallbladder microsomes were prostaglandin E2 and 6-keto-prostaglandin F1 alpha, which were increased four times over the levels demonstrated by normal gallbladder microsomes (p < 0.01). These data showed that inflammation of the human gallbladder was associated with increased synthesis of gallbladder 6-keto-prostaglandin F1 alpha and prostaglandin E2.
authors.affiliation: Department of Surgery, University of Texas Medical School, Houston 77030.
authors.forename: S I
authors.initials: SI
authors.lastname: Myers
authors.forename: L
authors.initials: L
authors.lastname: Bartula
mesh_terms: D015121:6-Ketoprostaglandin F1 alpha / Q000096:biosynthesis; D016718:Arachidonic Acid / Q000378:metabolism; D002764:Cholecystitis / Q000378:metabolism*; D015232:Dinoprostone / Q000096:biosynthesis*; D011464:Epoprostenol / Q000096:biosynthesis*; D005704:Gallbladder / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D006801:Humans; D007213:Indomethacin / Q000494:pharmacology; D007425:Intracellular Membranes / Q000378:metabolism; D008861:Microsomes / Q000378:metabolism
chemical_list: D016718:Arachidonic Acid; D015121:6-Ketoprostaglandin F1 alpha; D011464:Epoprostenol; D015232:Dinoprostone; D007213:Indomethacin