[Incorporation of [3H]alpha-tocopherol into isolated nuclei and its binding by rat liver chromatin].
The investigation of inclusion of [3H]alpha-tocopherol to isolated rat liver nuclei has revealed its nonspecific character. The presence of cytosol is necessary for specific interaction of alpha-tocopherol with nuclei. After the centrifugation of preliminarily labeled chromatin the most quantity of tocopherol was bound with oligonucleosomes and pelleted chromatin. It is supposed, that the preservation of supernucleosomes level of chromatin folding was necessary for the interaction of alpha-tocopherol with chromatin.
Petrova
G V
GV
Kapralov
A A
AA
Donchenko
G V
GV
D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism*; D002843:Chromatin / Q000378:metabolism*; D005260:Female; D066298:In Vitro Techniques; D008099:Liver / Q000378:metabolism*; D011485:Protein Binding; D051381:Rats; D014316:Tritium; D014810:Vitamin E / Q000378:metabolism*
D002843:Chromatin; D014316:Tritium; D014810:Vitamin E
Vkliuchenie [3H]alpha-tokoferola v izolirovannye iadra i ego sviazyvanie s khromatinom pecheni krys.