title: C-receptor ligand blocks pulmonary clearance of atrial natriuretic peptide in isolated rat lungs.
abstract: Pulmonary clearance of atrial natriuretic peptide (ANP) was measured by indicator dilution technique in isolated perfused rat lungs with and without ANP clearance receptor (C-receptor) blockade. Approximately 50% of a bolus injection of 125I-ANP was removed during a single pass through the lungs compared with the intravascular marker 14C-dextran. Pulmonary clearance of 125I-ANP was suppressed in a dose-dependent fashion by unlabeled ANP. C-receptor blockade suppressed pulmonary clearance of 125I-ANP to the same degree as unlabeled ANP. High-performance liquid chromatography analysis of the pulmonary venous effluent from lungs treated with C-receptor ligand demonstrated intact 125I-ANP. We conclude that virtually all of the pulmonary vascular uptake of 125I-ANP during a single pass through isolated lungs is secondary to removal by ANP C-receptors.
authors.affiliation: Division of Pulmonary and Critical Care Medicine, Rhode Island Hospital, Providence 02903.
authors.forename: J R
authors.initials: JR
authors.lastname: Klinger
authors.forename: R
authors.initials: R
authors.lastname: Moalli
authors.forename: R R
authors.initials: RR
authors.lastname: Warburton
authors.forename: D S
authors.initials: DS
authors.lastname: Wrenn
authors.forename: N S
authors.initials: NS
authors.lastname: Hill
mesh_terms: D000818:Animals; D009320:Atrial Natriuretic Factor / Q000378:metabolism* / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D007457:Iodine Radioisotopes; D008168:Lung / Q000187:drug effects* / Q000378:metabolism*; D008297:Male; D010446:Peptide Fragments / Q000378:metabolism / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017461:Receptors, Atrial Natriuretic Factor / Q000378:metabolism*
chemical_list: D007457:Iodine Radioisotopes; D010446:Peptide Fragments; C059320:SC 46542; D009320:Atrial Natriuretic Factor; D017461:Receptors, Atrial Natriuretic Factor