Sequence-specific interaction between S1F, a spinach nuclear factor, and a negative cis-element conserved in plastid-related genes.
The nuclear gene rps1 coding for the spinach plastid ribosomal protein CS1 exhibits both a constitutive and leaf-specific expression pattern. In contrast to other chloroplast-related genes like rbcS and cab, the leaf induction of rps1 expression is light-independent. These unique features of rps1 expression provide good models to study the mechanisms regulating plastid development and differentiation in higher plants. We report on the identification of a spinach leaf nuclear factor, designated S1F, interacting with the rps1 promoter. The S1F binding site is conserved in the promoter region of many plastid-related genes, including rbcS, cab, and rpl21. A binding activity similar to S1F was detected in nuclear extract from dark-grown de-differentiated soybean suspension cells. Through site-specific mutagenesis and transient expression in soybean cell protoplasts, we show that the S1F binding site is a negative element down-regulating the promoter activity of rps1. A ligated tetramer of S1F site was able to repress activity of the cauliflower mosaic virus 35 S promoter extending the negative function of the S1F binding site on promoter activity.
Zhou
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Laboratoire de Biologie Moléculaire Végétale, Centre National de la Recherche Scientifique, Unité de Recherche Associée 1178, Université J. Fourier, Grenoble, France.
Li
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YF
Rocipon
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Mache
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D001483:Base Sequence; D005075:Biological Evolution; D002467:Cell Nucleus / Q000502:physiology*; D015870:Gene Expression; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009687:Nuclear Proteins / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics*; D010944:Plants / Q000235:genetics*; D010957:Plasmids; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D015183:Restriction Mapping; D012269:Ribosomal Proteins / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D013025:Glycine max / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic
D009687:Nuclear Proteins; D010940:Plant Proteins; D012269:Ribosomal Proteins; C076261:RPS1 protein, Spinacia oleracea