[Mycoplasma restriction-modification system MunI and its possible role in pathogenesis processes].
The restriction-modification system, named RMMunI, has been purified and characterised from Friend murine erythroleukemia cells. The site-specific endonuclease recognizes and cleaves the 5'C1AATTG nucleotide sequence. RMunI is an isoschizomer of RMfeI from Mycoplasma fermentans. Site-specific methylase modifies the second adenine residue in the same sequence (5'Cam6ATTG). It was established that the discovered enzymatic system is from mycoplasma which contaminates cell lines. Mycoplasma's DNA hybridizes with species-specific DNA probed for Mycoplasma fermentans and Mycoplasma arginini. The possible role of mycoplasmic restriction-modification enzymes in the process of acquired immune deficiency syndrome are discussed.
Stakenas
P S
PS
Zaretskaia
N M
NM
Manelene
Z P
ZP
Mauritsas
M M
MM
Butkus
V V
VV
Ianulaĭtis
A A
AA
D000163:Acquired Immunodeficiency Syndrome / Q000201:enzymology; D000818:Animals; D010582:Bacteriophage lambda / Q000378:metabolism; D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D004279:DNA, Viral / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000302:isolation & purification / Q000378:metabolism*; D004587:Electrophoresis, Agar Gel; D004915:Leukemia, Erythroblastic, Acute / Q000201:enzymology; D051379:Mice; D008969:Molecular Sequence Data; D016858:Mycoplasma fermentans / Q000201:enzymology*; D014407:Tumor Cells, Cultured
D004269:DNA, Bacterial; D004279:DNA, Viral; C059776:endodeoxyribonuclease MfeI; D015252:Deoxyribonucleases, Type II Site-Specific
Mikoplazmennaia sistema restriktsii--modifikatsii MunI i ee vozmozhnaia rol' v protsessakh patogeneza.