[Tracer kinetics of amino acid].
In vivo turnover of an amino acid can be determined either by analysing the disappearance curve of a labeled amino acid after a single dose of labeled amino acid or by measuring the dilution of a continuously infused labeled amino acid at a steady state. From ethical concerns stable isotopically labeled amino acids, such as 13C-leucine and 15N-glycine have been routinely used to study amino acid kinetics with subsequent estimation of whole body protein turnover. Recently, instead of these 2 methods, pulse labeling by taking a single oral dose of labeled amino acid is more practical for the study of whole body protein turnover. We observed plasma 15N-glycine kinetics and urinary 15N excretion by a single oral dose of 15N-glycine simultaneously in healthy subjects and cirrhosis patients, and the results are discussed.
Hirayama
C
C
Saiseikai Gotsu General Hospital.
Kohno
M
M
Fujii
T
T
D000284:Administration, Oral; D000596:Amino Acids / Q000008:administration & dosage / Q000378:metabolism*; D002250:Carbon Radioisotopes; D005998:Glycine / Q000008:administration & dosage / Q000378:metabolism*; D006801:Humans; D007262:Infusions, Intravenous; D007930:Leucine / Q000008:administration & dosage / Q000378:metabolism*; D008103:Liver Cirrhosis / Q000378:metabolism; D009590:Nitrogen Radioisotopes; D011506:Proteins / Q000378:metabolism
D000596:Amino Acids; D002250:Carbon Radioisotopes; D009590:Nitrogen Radioisotopes; D011506:Proteins; D007930:Leucine; D005998:Glycine