title: Modified apolipoprotein pattern after irradiation of human high-density lipoproteins by ultraviolet B.
abstract: The ultraviolet B-induced destruction of tryptophan residues and lipid peroxidation of high-density lipoproteins is accompanied by the immediate and marked structural modification of the apolipoproteins, as revealed by SDS-polyacrylamide gel electrophoresis and immunoblot with specific monoclonal antibodies. Formation of several polymers of apolipoprotein A-I, apolipoprotein A-II or both apolipoproteins occurred, although apolipoprotein A-II did not contain any Trp residue. These results suggest that initial photochemical damage can be transferred via intramacromolecular processes to other sites within the same apolipoprotein and by intermacromolecular reactions from apolipoprotein A-I to other apolipoproteins. In both cases, lipid peroxidation enhances the propagation of the initial photochemical damage. The physiological significance of this work is discussed with respect to the low-light doses required for the alterations of the high-density lipoproteins.
authors.affiliation: Muséum National d'Histoire Naturelle, Laboratoire de Physico-Chimie de l'Adaptation Biologique, Paris, France.
authors.forename: S
authors.initials: S
authors.lastname: Salmon
authors.forename: R
authors.initials: R
authors.lastname: Santus
authors.forename: J C
authors.initials: JC
authors.lastname: Mazière
authors.forename: M
authors.initials: M
authors.lastname: Aubailly
authors.forename: J
authors.initials: J
authors.lastname: Haigle
mesh_terms: D016632:Apolipoprotein A-I / Q000737:chemistry; D016633:Apolipoprotein A-II / Q000737:chemistry; D001053:Apolipoproteins / Q000737:chemistry*; D006801:Humans; D015227:Lipid Peroxidation; D008075:Lipoproteins, HDL / Q000737:chemistry / Q000528:radiation effects*; D015394:Molecular Structure; D010777:Photochemistry; D012867:Skin / Q000528:radiation effects; D013471:Sunburn; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis; D014364:Tryptophan / Q000528:radiation effects
chemical_list: D016632:Apolipoprotein A-I; D016633:Apolipoprotein A-II; D001053:Apolipoproteins; D008075:Lipoproteins, HDL; D017392:Thiobarbituric Acid Reactive Substances; D014364:Tryptophan