[A comparison of 3 methods of lipoprotein (a) isolation from human plasma].
Three methods for purification of lipoprotein (a) [Lp(a)] from human plasma were compared. Method I: two-stage ultracentrifugation with subsequent gel-filtration of Lp(a) containing fractions (1.063-1.090 g/ml) on Sepharose CL-4B. Method II: ultracentrifugation followed by affinity chromatography of plasma fraction (1.063 g/ml) on anti-apoB sorbent. Method III: affinity chromatography of the whole plasma on anti-apo(a) sorbent. The Lp(a) yield of these methods is 35, 54 and 41%, respectively. The method III is preferable of these three because it permitted high purification of a large amount of Lp(a) by single-step chromatography.
Afanas'eva
O I
OI
Adamova
I Iu
IIu
Benevodenskaia
G F
GF
Pokrovskiĭ
S N
SN
D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D006801:Humans; D007164:Immunosorbents; D017270:Lipoprotein(a) / Q000097:blood*; D010956:Plasmapheresis; D014461:Ultracentrifugation
D007164:Immunosorbents; D017270:Lipoprotein(a)
Sravnenie trekh metodov vydeleniia lipoproteida (a) na plazmy krovi cheloveka.