High-performance liquid chromatographic-mass spectrometric assay of busulfan in serum and cerebrospinal fluid.
A liquid chromatographic-mass spectrometric method has been developed to determine busulfan concentrations in the cerebrospinal fluid and serum of some children undergoing bone marrow autotransplantation. After two liquid-liquid extraction steps with dichloromethane on a biological matrix, the separation of busulfan was carried out by isocratic reversed-phase chromatography. The mass spectrometric system was operated in electron-impact mode. Principal ions at m/z 175, 111 and 79 were observed for busulfan, but only m/z 175 was chosen for the quantification of the analyte. The retention time of busulfan was 2.5 min. The detection limit of 100 ng/ml allowed the determination of cerebrospinal fluid and serum busulfan concentrations during the four days of high-dose (1 mg/kg) treatment prior to autotransplantation in five child patients.
Pichini
S
S
Istituto Superiore di Sanita, Rome, Italy.
Altieri
I
I
Bacosi
A
A
Di Carlo
S
S
Zuccaro
P
P
Iannetti
P
P
Pacifici
R
R
D002066:Busulfan / Q000032:analysis* / Q000097:blood / Q000134:cerebrospinal fluid; D002138:Calibration; D002648:Child; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000097:blood / Q000134:cerebrospinal fluid; D013058:Mass Spectrometry / Q000379:methods*; D015203:Reproducibility of Results
D002066:Busulfan