Determination of dextropropoxyphene and norpropoxyphene in plasma by high-performance liquid chromatography.
A simple and sensitive procedure for the routine assay of the analgesic drug dextropropoxyphene and its main metabolite, norpropoxyphene, in plasma is described. After liquid-liquid extraction from alkalinized plasma and back-extraction into a small volume of an acidic aqueous phase, the aqueous phase was injected into a column packed with 3-microns octadecylsilica particles. Ultraviolet absorbance detection at 210 nm was used. Concentrations down to 2 nM could be determined for both compounds; at this level, the intra-assay coefficient of variation was 5%.
Pettersson
K J
KJ
Department of Bioanalysis, Astra Arcus AB, Södertälje, Sweden.
Nilsson
L B
LB
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D011431:Dextropropoxyphene / Q000031:analogs & derivatives* / Q000097:blood*; D006801:Humans; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet
C002756:norpropoxyphene; D011431:Dextropropoxyphene