Identification of amino acid residues of rat angiotensin II receptor for ligand binding by site directed mutagenesis.
To determine the specific mechanism of ligand binding to angiotensin (Ang II) receptor AT1, mutagenized rat receptor cDNAs were expressed transiently in COS-7 cells and the effect of the mutations on the binding to peptidic and non-peptidic ligands was analyzed by Scatchard plots. Mutation of Lys199 to Gln in the intramembrane domain strongly reduced the affinity to both [125I] Ang II and [125I]-1Sar, 8Ile-Ang II whereas mutation of two other Lys had little effect, indicating involvement of Lys199 in binding ligands. Replacement of each of four Cys in the extracellular domain markedly reduced binding affinity, indicating the importance of two putative disulfide bridges in the formation of active receptor conformation. Substitution of Asp for Asn in N-glycosylation had no effect on ligand binding or expression of the receptor. These studies indicate mutated receptors are expressed in the plasma membrane and are amenable for further detailed studies.
Yamano
Y
Y
Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN 37232.
Ohyama
K
K
Chaki
S
S
Guo
D F
DF
Inagami
T
T
D000804:Angiotensin II / Q000378:metabolism*; D000818:Animals; D001665:Binding Sites; D004220:Disulfides / Q000737:chemistry; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000378:metabolism; D016297:Mutagenesis, Site-Directed; D051381:Rats; D011945:Receptors, Angiotensin / Q000737:chemistry / Q000378:metabolism*; D013329:Structure-Activity Relationship
D004220:Disulfides; D008562:Membrane Glycoproteins; D011945:Receptors, Angiotensin; D000804:Angiotensin II