p53 point mutations in primary human gastric carcinomas.
p53 point mutations in primary gastric carcinomas were analyzed by performing cDNA deoxynucleotide sequencing of the gene. Out of 16, 9 (56.3%) primary gastric carcinoma cases, including early cancer, showed one or more p53 point mutations in their open-reading frame, and 4 out of 9 cases had a p53 point mutation within highly conserved domains. The characteristics of the p53 mutation spectrum observed in primary tumors were (a) frequent mutation at an A:T pair (50%, 7 out of 14 mutations), (b) high transversion incidence (29%, 4 out of 14 mutations), (c) no transition at CpG, and (d) no G:C to T:A transversion. Our results suggest that p53 mutation is a common event in gastric carcinoma occurring from the early stage of progression with its specific mutation spectrum.
Yokozaki
H
H
Department of Pathology, Hiroshima University School of Medicine, Japan.
Kuniyasu
H
H
Kitadai
Y
Y
Nishimura
K
K
Todo
H
H
Ayhan
A
A
Yasui
W
W
Ito
H
H
Tahara
E
E
D000328:Adult; D000368:Aged; D001483:Base Sequence; D002277:Carcinoma / Q000235:genetics*; D005260:Female; D016158:Genes, p53; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D017354:Point Mutation; D013274:Stomach Neoplasms / Q000235:genetics*