title: In vitro and in vivo effects of the anti-inflammatory peptides, antiflammins.
abstract: The anti-inflammatory synthetic polypeptides referred to as antiflammins are thought to be inhibitors of phospholipase A2 (PLA2) (EC 3.1.1.4). These peptides are from the sequence of amino acids of greatest similarity between uteroglobin and lipocortin I. The effect of these peptides was studied on PLA2 activation in rat platelets and on acute inflammatory models after local or parenteral administration of drug. We found that antiflammins decreased collagen-induced platelet activation, but had no effect when arachidonic acid was used as activator. The peptides were able to inhibit acute inflammatory processes induced by carrageenan or phorbol esters when administered locally or parenterally. However, antiflammins had no effect on inflammation induced by exogenous PLA2 administration. These results indicate that the antiflammins may have a direct inhibitory effect on PLA2 activation but not on the enzyme or enzyme-substrate interaction.
authors.affiliation: Departamento de Ciencias Fisiológicas, Facultad de Farmacia, Universidad de Barcelona, Spain.
authors.forename: S
authors.initials: S
authors.lastname: Lloret
authors.forename: J J
authors.initials: JJ
authors.lastname: Moreno
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000893:Anti-Inflammatory Agents; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000008:administration & dosage / Q000494:pharmacology*; D016718:Arachidonic Acid; D002351:Carrageenan; D003094:Collagen; D003907:Dexamethasone / Q000494:pharmacology; D007660:Ketoprofen / Q000494:pharmacology; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000494:pharmacology*; D010446:Peptide Fragments / Q000494:pharmacology*; D010741:Phospholipases A / Q000037:antagonists & inhibitors / Q000737:chemistry; D054467:Phospholipases A2; D010974:Platelet Aggregation / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D013929:Thromboxane B2 / Q000032:analysis; D014598:Uteroglobin / Q000494:pharmacology*
chemical_list: D000893:Anti-Inflammatory Agents; D000894:Anti-Inflammatory Agents, Non-Steroidal; D009842:Oligopeptides; D010446:Peptide Fragments; C064645:antiflammin P1; C064647:antiflammin P2; D016718:Arachidonic Acid; D013929:Thromboxane B2; D003907:Dexamethasone; D002351:Carrageenan; D003094:Collagen; D014598:Uteroglobin; D007660:Ketoprofen; D010741:Phospholipases A; D054467:Phospholipases A2