[Determination of procollagen type III aminopropeptide by a solid phase method using antibodies conjugated with peroxidase or protein A labelled with radioactive iodine].
A solid-phase assay was developed for estimation of procollagen III aminopropeptide which involved both immunoenzymatic and radioimmune procedures. The assay did not require the iodine-labelled aminopropeptide, while content of the antigen was estimated using the conjugate of secondary antibodies and peroxidase or iodine-labelled protein A. Substitution of the protein A for the antibodies conjugate and peroxidase enabled to abolish the radioactive materials use.
Planutis
K S
KS
Morozevich
G E
GE
Berman
A E
AE
D000818:Animals; D002417:Cattle; D006735:Horseradish Peroxidase; D007124:Immunoenzyme Techniques; D007457:Iodine Radioisotopes; D010446:Peptide Fragments / Q000378:metabolism*; D011347:Procollagen / Q000378:metabolism*; D013205:Staphylococcal Protein A
D007457:Iodine Radioisotopes; D010446:Peptide Fragments; D011347:Procollagen; D013205:Staphylococcal Protein A; C041876:procollagen Type III-N-terminal peptide; D006735:Horseradish Peroxidase
Opredelenie aminopropeptida prokollagena III tipa tverdofaznym metodom s ispol'zovaniem kon''iugata antitel s peroksidazoĭ ili belka A, mechennogo radioaktivnym ĭodom.