Effects of surgical decapitation and chicken growth hormone (cGH) replacement therapy on chick embryo growth.
The effects of decapitation and chicken growth hormone (cGH) replacement therapy on chick embryo growth has been investigated. Removal of the prosencephalon at 33-38 hrs (1.38 to 1.58 Days) of incubation decreased body (torso) and liver weights as well as skeletal growth as indicated by tibial length. A single pituitary gland transplanted onto the chorioallantoic membrane (CAM) partially restored torso growth and completely reversed the increase in body water content which characterizes decapitated embryos. Replacement therapy with cGH did not influence body weight but did, on Day 16.5 of incubation, increase tibial length and liver DNA content and concentration. These latter findings suggest that there may be limited hypothalamoadenohypophyseal (GH) axis function in the chick embryo. The effects of decapitation on torso growth are also discussed in conjunction with decapitation effects on albumen swallowing (absorption) and yolk absorption.
Thommes
R C
RC
Laboratory of Developmental Endocrinology, Sarasota, FL 34239.
Woods
J E
JE
Aramburo
C
C
Buonomo
F C
FC
Scanes
C G
CG
D000418:Albumins / Q000032:analysis; D000818:Animals; D001699:Biometry; D001835:Body Weight / Q000187:drug effects; D002642:Chick Embryo; D004247:DNA / Q000032:analysis; D004622:Embryo, Mammalian / Q000033:anatomy & histology; D004625:Embryo, Nonmammalian; D005314:Embryonic and Fetal Development / Q000187:drug effects*; D005269:Femur / Q000033:anatomy & histology / Q000196:embryology; D006003:Glycogen / Q000032:analysis; D013006:Growth Hormone / Q000494:pharmacology*; D006257:Head / Q000601:surgery*; D007031:Hypothalamus / Q000502:physiology; D008099:Liver / Q000033:anatomy & histology / Q000737:chemistry / Q000187:drug effects; D009929:Organ Size / Q000187:drug effects / Q000502:physiology; D010902:Pituitary Gland / Q000502:physiology; D013977:Tibia / Q000033:anatomy & histology / Q000196:embryology; D015017:Yolk Sac / Q000033:anatomy & histology
D000418:Albumins; D013006:Growth Hormone; D006003:Glycogen; D004247:DNA