title: A simple, rapid method for purification of epsilon-subunit, coupling factor 6, subunit d, and subunit e from rat liver H(+)-ATP synthase and determination of the complete amino acid sequence of epsilon-subunit.
abstract: The rat liver mitochondrial epsilon-subunit, coupling factor 6, subunit d, and subunit e of H(+)-ATP synthase, which are all extra subunits with no counterparts in Escherichia coli, were purified by reverse-phase high performance liquid chromatography. The complete amino acid sequence of the rat epsilon-subunit was determined by automated Edman degradation of the whole protein and derived peptides. The protein contains 50 amino acids and has a molecular mass of 5635 kDa. It is a basic hydrophilic protein with an isoelectric point of 10.5. The sequence of the rat epsilon-subunit is highly homologous with that of the epsilon-subunit of bovine heart and slightly similar to those of the epsilon-subunit of the yeast and sweet potato mitochondria. However, it has no homology with any subunit of bacterial or chloroplast H(+)-ATP synthase.
authors.affiliation: Faculty of Pharmaceutical Sciences, University of Tokushima, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Higuti
authors.forename: Y
authors.initials: Y
authors.lastname: Yoshihara
authors.forename: K
authors.initials: K
authors.lastname: Kuroiwa
authors.forename: Y
authors.initials: Y
authors.lastname: Kawamura
authors.forename: H
authors.initials: H
authors.lastname: Toda
authors.forename: F
authors.initials: F
authors.lastname: Sakiyama
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D008930:Mitochondria, Liver / Q000201:enzymology*; D008969:Molecular Sequence Data; D006180:Proton-Translocating ATPases / Q000737:chemistry / Q000302:isolation & purification*; D051381:Rats; D016415:Sequence Alignment
chemical_list: D006180:Proton-Translocating ATPases