title: [Isolation of protoplasts of Xanthomonas rubrilineans and their use in the study of localization of aminopeptidases].
abstract: The culture of Xanthomonas rubrilineans was able to synthesize a number of intracellular aminopeptidases. To study localization of the enzymes in the cells, a protoplasting procedure was developed providing the yield of 99.7 per cent. The following subcellular fractions were isolated: periplasmic, cytoplasmic and membranous. It was shown that alanine aminopeptidase was a cytoplasmic enzyme and glutamate peptidase was a membrane-bound enzyme.
authors.forename: G N
authors.initials: GN
authors.lastname: Telesnina
authors.forename: N A
authors.initials: NA
authors.lastname: Vasil'eva
authors.forename: I N
authors.initials: IN
authors.lastname: Krest'ianova
authors.forename: Iu E
authors.initials: IuE
authors.lastname: Bartoshevich
authors.forename: Iu O
authors.initials: IuO
authors.lastname: Sazykin
mesh_terms: D000626:Aminopeptidases / Q000096:biosynthesis* / Q000302:isolation & purification; D002462:Cell Membrane / Q000201:enzymology; D002498:Centrifugation; D002850:Chromatography, Gel; D003470:Culture Media; D003593:Cytoplasm / Q000201:enzymology; D066298:In Vitro Techniques; D011523:Protoplasts / Q000166:cytology / Q000201:enzymology* / Q000648:ultrastructure; D014974:Xanthomonas / Q000166:cytology* / Q000201:enzymology / Q000254:growth & development
chemical_list: D003470:Culture Media; D000626:Aminopeptidases
vernacular_title: Poluchenie protoplastov Xanthomonas rubrilineans i ikh ispol'zovanie dlia izucheniia lokalizatsii aminopeptidaz.