title: Low molecular mass phosphoproteins from the frog rod outer segments form a complex with 48 kDa protein.
abstract: Upon separation of cAMP-dependent low molecular mass phosphoproteins [Components I and II; Polans et al. (1979) J. gen. Physiol. 74, 595-613] from the frog rod outer segments by gel-chromatography, isoelectric focusing, non-denaturating electrophoresis and ion-exchange chromatography, they behave like subunits of the oligomeric complex. Apparent molecular mass of the complex determined by gel-chromatography is 52-57 kDa and by non-denaturating gradient electrophoresis is 62-66 kDa. The isoelectric point of the complex is 5.5. The elution profile of Components I and II upon gel-chromatography and ion-exchange chromatography coincides with that of major rod outer segment 48 kDa protein. The isoelectric point for them also coincides with the isoelectric point of 48 kDa protein. The amount of low molecular mass phosphoproteins is sealed rods is equal to one molecule per 60 rhodopsin molecules and coincides with that of a 48 kDa protein. It is suggested that in solution Components I and II form an oligomeric complex with 48 kDa protein.
authors.affiliation: Laboratory of Biophysics of Sensory Reception, Russian Academy of Sciences, Pushchino, Moscow Region.
authors.forename: G B
authors.initials: GB
authors.lastname: Krapivinsky
authors.forename: A L
authors.initials: AL
authors.lastname: Malenyov
authors.forename: I V
authors.initials: IV
authors.lastname: Zaikina
authors.forename: E E
authors.initials: EE
authors.lastname: Fesenko
mesh_terms: D000818:Animals; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D066298:In Vitro Techniques; D007526:Isoelectric Point; D046911:Macromolecular Substances; D008970:Molecular Weight; D010750:Phosphoproteins / Q000737:chemistry* / Q000302:isolation & purification; D011487:Protein Conformation; D011896:Rana temporaria; D012374:Rod Cell Outer Segment / Q000737:chemistry*; D012995:Solubility
chemical_list: D046911:Macromolecular Substances; D010750:Phosphoproteins