Identification of functionally active fragments of staphylococcal enterotoxin B.
It has been found that staphylococcal enterotoxin B contains a proteolysis-sensitive sequence in the cysteine loop formed by two half-cystines located in the middle of the toxin polypeptide chain. Fragments of the enterotoxin formed as a result of its digestion in this region have been isolated, their N-terminal sequences have been determined and sites of proteolysis have been identified. It has been demonstrated that the N-terminal fragment of staphylococcal enterotoxin B is capable of activating T cell proliferation in the culture of human mononuclear cells practically to the same degree as the intact enterotoxin. The toxin's C-terminal fragment possesses an ability to activate calmodulin-dependent enzymes and is probably the toxicogenic part of the enterotoxin.
Alakhov VYu
Research Center of Molecular Diagnostics and Therapy, Moscow, Russia.
Klinsky EYu
Kolosov
M I
MI
Maurer-Fogy
I
I
Moskaleva EYu
Sveshnikov
P G
PG
Pozdnyakova
L P
LP
Shemchukova
O B
OB
Severin
E S
ES
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D004768:Enterotoxins / Q000737:chemistry* / Q000494:pharmacology; D006801:Humans; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry; D012689:Sequence Homology, Nucleic Acid; D013211:Staphylococcus aureus / Q000378:metabolism*; D013601:T-Lymphocytes / Q000166:cytology / Q000187:drug effects
D000911:Antibodies, Monoclonal; D004768:Enterotoxins; D010446:Peptide Fragments; C031818:enterotoxin B, staphylococcal