Cloning of a major heparan sulfate proteoglycan from brain and identification as the rat form of glypican.
We have obtained the complete coding sequence of a highly conserved heparan sulfate proteoglycan which we previously characterized biochemically after isolation from rat brain. An open reading frame of 558 amino acids encodes a protein with a molecular mass of 62 kDa containing three peptide sequences present in the isolated proteoglycan. The total sequence obtained is 3.5 kb long, including 1.6 kb of 3'-untranslated sequence and 0.2 kb of 5'-untranslated sequence. The deduced amino acid sequence and the 3'- and 5'-untranslated sequences have 89% and 66-80% identity, respectively, with those of a phosphatidylinositol-anchored human lung fibroblast heparan sulfate proteoglycan (glypican) for which mRNA is detectable in a large number of human cell lines. Our data therefore demonstrate that this major heparan sulfate proteoglycan of brain is the rat form of glypican.
Karthikeyan
L
L
Department of Pharmacology, New York University Medical Center, NY 10016.
Maurel
P
P
Rauch
U
U
Margolis
R K
RK
Margolis
R U
RU
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000502:physiology*; D003001:Cloning, Molecular / Q000379:methods; D004247:DNA / Q000235:genetics; D015723:Gene Library; D019812:Heparan Sulfate Proteoglycans; D006497:Heparan Sulfate / Q000235:genetics*; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D016376:Oligonucleotides, Antisense; D016366:Open Reading Frames; D010446:Peptide Fragments / Q000737:chemistry; D016133:Polymerase Chain Reaction; D011509:Proteoglycans / Q000235:genetics*; D051381:Rats
D004247:DNA; D019812:Heparan Sulfate Proteoglycans; D006497:Heparan Sulfate; D009838:Oligodeoxyribonucleotides; D016376:Oligonucleotides, Antisense; D010446:Peptide Fragments; D011509:Proteoglycans