title: Preliminary crystallographic studies of urease from jack bean and from Klebsiella aerogenes.
abstract: Ureases from both jack bean (Canavalia ensiformis) seeds and Klebsiella aerogenes have been crystallized by the hanging drop method. The plant-derived urease crystals are regular octahedra analogous to those obtained by Sumner. Preliminary X-ray diffraction studies show that the crystals belong to the cubic space group F4(1)32, with a = 364 A, and appear to contain one or two subunits in the asymmetric unit. Using a synchrotron source, the crystals diffract to near 3.5 A resolution. Crystals of urease from K. aerogenes belong to the cubic space group I23 or I2(1)3, with a = 170.8 A and appear to contain a single catalytic unit per asymmetric unit. The crystals diffract to better than 2.0 A resolution and are well suited for structural analysis.
authors.affiliation: Section of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, NY 14853.
authors.forename: E
authors.initials: E
authors.lastname: Jabri
authors.forename: M H
authors.initials: MH
authors.lastname: Lee
authors.forename: R P
authors.initials: RP
authors.lastname: Hausinger
authors.forename: P A
authors.initials: PA
authors.lastname: Karplus
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry*; D007887:Fabaceae / Q000201:enzymology*; D007711:Klebsiella pneumoniae / Q000201:enzymology*; D010940:Plant Proteins / Q000737:chemistry*; D010946:Plants, Medicinal; D014510:Urease / Q000737:chemistry*; D014961:X-Ray Diffraction
chemical_list: D001426:Bacterial Proteins; D010940:Plant Proteins; D014510:Urease