The metabolism of 1,3-dinitrobenzene by rat testicular subcellular fractions.
The metabolism of 1,3-dinitrobenzene by rat testicular subcellular fractions (microsomes, cytosol or 9000 x g supernatant (S-9)) was studied. The effects of NADPH, oxygen, glutathione (GSH) and carbon monoxide upon the rate of metabolism were determined. Three metabolites were identified, and characterised as 3-nitrosonitrobenzene, 3-nitrophenylhydroxylamine and 3-nitroaniline by co-chromatography with authentic standards. These findings indicate the presence of an inherent enzyme system capable of facilitating nitro-reduction in the testes. The implications of these findings to the mechanism of 1,3-DNB-induced testicular toxicity are discussed.
Ellis
M K
MK
Biochemical Toxicology Section, ICI Central Toxicology Laboratory, Macclesfield, Cheshire, UK.
Foster
P M
PM
D000818:Animals; D002248:Carbon Monoxide / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D046508:Culture Techniques; D004136:Dinitrobenzenes / Q000378:metabolism* / Q000633:toxicity; D005978:Glutathione / Q000494:pharmacology; D008297:Male; D009249:NADP / Q000494:pharmacology; D009578:Nitrobenzenes / Q000032:analysis; D009603:Nitroso Compounds / Q000032:analysis; D010100:Oxygen / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D013737:Testis / Q000187:drug effects / Q000378:metabolism*
D004136:Dinitrobenzenes; D009578:Nitrobenzenes; D009603:Nitroso Compounds; C063131:1-nitro-3-nitrosobenzene; D009249:NADP; D002248:Carbon Monoxide; C017906:3-dinitrobenzene; D005978:Glutathione; D010100:Oxygen