Localization of an immunodominant domain on baculovirus-produced parvovirus B19 capsids: correlation to a major surface region on the native virus particle.
An immunodominant region on baculovirus-produced parvovirus B19 VP2 capsids was localized between amino acids 259 and 426 by mapping the binding sites of a panel of monoclonal antibodies which recognize determinants on the particles. The binding sites of three monoclonal antibodies were fine-mapped within this antigenic domain. Six VP2-specific monoclonal antibodies recognized determinants common to both the empty capsids and native parvovirus. The defined antigenic region is most probably exposed on the native B19 virion and corresponds to part of the threefold spike on the surface of canine parvovirus particles.
Brown
C S
CS
Department of Virology, Faculty of Medicine, Leiden University, The Netherlands.
Jensen
T
T
Meloen
R H
RH
Puijk
W
W
Sugamura
K
K
Sato
H
H
Spaan
W J
WJ
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D016367:Baculoviridae / Q000235:genetics*; D015153:Blotting, Western; D002213:Capsid / Q000032:analysis / Q000235:genetics / Q000276:immunology*; D002460:Cell Line; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D007313:Insecta; D008969:Molecular Sequence Data; D010321:Parvoviridae / Q000235:genetics / Q000276:immunology*; D010957:Plasmids; D011993:Recombinant Fusion Proteins / Q000032:analysis / Q000276:immunology; D015183:Restriction Mapping; D014162:Transfection
D000911:Antibodies, Monoclonal; D011993:Recombinant Fusion Proteins