title: Nagstatin, a new inhibitor of N-acetyl-beta-D-glucosaminidase, produced by Streptomyces amakusaensis MG846-fF3. Taxonomy, production, isolation, physico-chemical properties and biological activities.
abstract: Nagstatin, a new inhibitor of N-acetyl-beta-D-glucosaminidase (NAG-ase) was discovered in the fermentation broth of Streptomyces amakusaensis MG846-fF3. It was purified by chromatography on Dowex 50W, Avicel and Sephadex LH-20 followed by the treatment of active carbon and then isolated as colorless powder. Nagstatin has the molecular formula of C12H17N3O6. It is competitive with the substrate, and the inhibition constant (Ki) was 1.7 x 10(-8) M.
authors.affiliation: Institute of Microbial Chemistry, Tokyo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Aoyagi
authors.forename: H
authors.initials: H
authors.lastname: Suda
authors.forename: K
authors.initials: K
authors.lastname: Uotani
authors.forename: F
authors.initials: F
authors.lastname: Kojima
authors.forename: T
authors.initials: T
authors.lastname: Aoyama
authors.forename: K
authors.initials: K
authors.lastname: Horiguchi
authors.forename: M
authors.initials: M
authors.lastname: Hamada
authors.forename: T
authors.initials: T
authors.lastname: Takeuchi
mesh_terms: D000118:Acetylglucosaminidase / Q000037:antagonists & inhibitors*; D000818:Animals; D051379:Mice; D011720:Pyrazoles / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D011725:Pyridines / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D013302:Streptomyces / Q000737:chemistry* / Q000235:genetics
chemical_list: D011720:Pyrazoles; D011725:Pyridines; C077641:nagstatin; D000118:Acetylglucosaminidase