title: [The effect of high-dose ultraviolet irradiation on sodium, calcium and aldosterone in the blood of calves].
abstract: Five Holstein-Friesian calves, from one sire, with prevalent black hair coat pigmentation were used in the experiment. The mean age was 33 days and the mean live weight 51 kg. The animals were exposed free running without interruption for 12 hours to an artificial ultraviolet light in the range of 280-320 nm. The mean doses of radiation was 179.10(-10) J/h/m. One-spot high-pressure mercury discharge lamps Tesla RVK 400 W were used as a radiation source. The dose rate was estimated from measurements by a spectral photometer with filter UG 2 for absorbtion of visible light located at the height of the back of standing calf. Blood samples were collected immediately before the beginning of treatment and after 5, 12, 24, 48 and 72 hours. The blood plasma aldosterone was measured by radioimmunoassays, the levels of sodium, potassium and calcium in blood plasma by flame spectrophotometry. Double classification variance analysis and evaluation according to the Snedecor F-test, the contrast effect test according to Duncan and regression analysis were used for statistical evaluation. Compared to the first sampling, sodium increased significantly after 5 and 12 hours of exposure (Tab. I) to 138.1 and 138.3 mmol/l, respectively. In the subsequent samplings this trend continued up to 72 hours from the beginning of irradiation (140.5 mmol/l). The potassium level did not change statistically significantly. Owing to an excessive irradiation, the calcium concentration increased significantly. The greatest increase occurred after 12 hours of irradiation (from 2.29 mmol/l to 2.61 mmol/l) and after 36 hours from the end of irradiation (2.70 mmol/l).(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Výskumný ústav zivocísnej výroby, Nitra.
authors.forename: J
authors.initials: J
authors.lastname: Broucek
authors.forename: D
authors.initials: D
authors.lastname: Gajdosík
authors.forename: M
authors.initials: M
authors.lastname: Letkovicová
authors.forename: K
authors.initials: K
authors.lastname: Kovalcik
mesh_terms: D000367:Age Factors; D000450:Aldosterone / Q000097:blood* / Q000528:radiation effects; D000818:Animals; D002118:Calcium / Q000097:blood* / Q000528:radiation effects; D002417:Cattle / Q000097:blood*; D011829:Radiation Dosage; D012964:Sodium / Q000097:blood* / Q000528:radiation effects; D014466:Ultraviolet Rays
chemical_list: D000450:Aldosterone; D012964:Sodium; D002118:Calcium
vernacular_title: Pôsobenie nadmerného ultrafialového ziarenia na sodík, vápnik a aldosteron v plazme teliat.