The mouse alpha 1(XII) and human alpha 1(XII)-like collagen genes are localized on mouse chromosome 9 and human chromosome 6.
Type XII collagen is a member of the FACIT (fibril-associated collagens with interrupted triple helices) group of extracellular matrix proteins. Like the other members of this group, collagen types IX and XIV, type XII has alternating triple-helical and non-triple-helical domains. Because of its structure, its association with collagen fibrils, and its distribution in dense connective tissues, type XII is thought possibly to act as a cross-bridge between fibrils and resist shear forces caused by tension. A portion of the ffuse gene was isolated by screening a genomic library with a chicken alpha 1 (XII) cDNA probe, followed by subcloning and sequence analysis. Comparison of exon sequences with the sequence of a mouse cDNA clone allowed the mouse gene to be identified as the alpha 1 (XII) collagen gene. In the mouse, Col12a1 is located on chromosome 9, as determined by linkage analysis using DNA from interspecific backcrosses with Mus spretus. Screening of a human genomic library also allowed the isolation of a human alpha 1(XII)-like gene (CoL12A1). This gene was mapped to chromosome 6 by blot hybridization to DNA from human/hamster hybrid cell lines. This information should prove useful in determining the role of type XII collagen genes as candidate genes in inheritable connective tissue diseases.
Oh
S P
SP
Department of Anatomy and Cellular Biology, Harvard Medical School, Boston, Massachusetts 02115.
Taylor
R W
RW
Gerecke
D R
DR
Rochelle
J M
JM
Seldin
M F
MF
Olsen
B R
BR
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002896:Chromosomes, Human, Pair 6; D003094:Collagen / Q000235:genetics*; D006224:Cricetinae; D004247:DNA; D015342:DNA Probes; D006801:Humans; D006822:Hybrid Cells; D051379:Mice; D008809:Mice, Inbred C3H; D008811:Mice, Inbred DBA; D008969:Molecular Sequence Data; D009115:Muridae / Q000235:genetics*; D016133:Polymerase Chain Reaction; D015183:Restriction Mapping
D015342:DNA Probes; D003094:Collagen; D004247:DNA