title: Interaction of bovine serum albumin with the surface of a microcrystalline aluminum oxide hydroxide compound: a possible new type of phosphate adsorbent.
abstract: Aluminum hydroxide gel (ALG) has been effective for ameliorating acidosis associated with phosphatemia caused by hemodialysis. However, aluminum accumulation in the body causes severe side effects. As substitute for ALG, a new type of aluminum oxide hydroxide (tentatively named PT-A) was prepared with the hope of future clinical use. PT-A has a microcrystalline structure with a high resistance to pH change and has more phosphate-binding efficacy than ALG. It was tested for possible interaction with protein by adsorption test, zeta-potential analysis, X-ray diffraction, and scanning electron microscopy. Bovine serum albumin (BSA) was chosen as a model protein. The interaction of BSA with PT-A depended on the amount of adsorbent. Protein adsorption occurred rapidly and reached the maximal level at near neutral pHs. Phosphate adsorption was not affected by the presence of BSA, but the interaction of BSA with PT-A was significantly reduced by the presence of phosphate. Zeta-potential changes on the surface of PT-A indicated that the positively charged surface of PT-A was covered with negatively charged phosphate ions that repelled negatively charged BSA molecules. X-ray diffraction patterns indicated no observable structural alteration caused by adsorption of BSA or phosphate, and scanning electron microscopy revealed that BSA covered the outer surface of PT-A but did not cover small pores, where phosphate can freely penetrate.
authors.affiliation: Faculty of Pharmaceutical Sciences, University of Tokushima, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Nishida
authors.forename: A
authors.initials: A
authors.lastname: Ookubo
authors.forename: Y
authors.initials: Y
authors.lastname: Hashimura
authors.forename: A
authors.initials: A
authors.lastname: Ikawa
authors.forename: Y
authors.initials: Y
authors.lastname: Yoshimura
authors.forename: K
authors.initials: K
authors.lastname: Ooi
authors.forename: T
authors.initials: T
authors.lastname: Suzuki
authors.forename: Y
authors.initials: Y
authors.lastname: Tomita
authors.forename: J
authors.initials: J
authors.lastname: Kawada
mesh_terms: D000327:Adsorption; D000536:Aluminum Hydroxide / Q000737:chemistry* / Q000494:pharmacology; D000537:Aluminum Oxide / Q000737:chemistry* / Q000494:pharmacology; D000818:Animals; D002417:Cattle; D004347:Drug Interactions; D006863:Hydrogen-Ion Concentration; D010710:Phosphates / Q000737:chemistry / Q000493:pharmacokinetics; D011506:Proteins / Q000737:chemistry / Q000493:pharmacokinetics; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000493:pharmacokinetics*; D013499:Surface Properties
chemical_list: D010710:Phosphates; D011506:Proteins; D012710:Serum Albumin, Bovine; D000536:Aluminum Hydroxide; C069471:aluminum oxide hydroxide; D000537:Aluminum Oxide