Ion-spray mass spectrometric analysis of glycosaminoglycan oligosaccharides.
Oligosaccharides from hyaluronic acid and chondroitin 6-sulfate were prepared by digestion with testicular hyaluronidase and separated according to their degree of polymerization by gel-permeation chromatography. These materials were successively analyzed by negative-mode ion-spray mass spectrometry with an atmospheric-pressure ion source. An ion-spray interface was used to produce ions via the ion evaporation process, producing mass spectra containing a series of molecular species carrying multiple charges. Using two adjacent multiply charged molecular ions, the exact molecular weights up to the tetradecasaccharide were calculated with a precision of +/- 1 dalton. This type of mass spectrometry was also demonstrated to be feasible for the analysis of mixtures of oligosaccharides, including tetra-, hexa-, octa- and decasaccharides, from hyaluronic acid or chondroitin 6-sulfate without separation. Ion-spray mass spectrometry was thus shown to be applicable to the structural analysis of oligosaccharides from glycosaminoglycans.
Takagaki
K
K
Department of Biochemistry, Hirosaki University School of Medicine, Japan.
Kojima
K
K
Majima
M
M
Nakamura
T
T
Kato
I
I
Endo
M
M
D000818:Animals; D002240:Carbohydrate Sequence; D002809:Chondroitin Sulfates / Q000737:chemistry; D006025:Glycosaminoglycans / Q000737:chemistry*; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009844:Oligosaccharides / Q000737:chemistry
D006025:Glycosaminoglycans; D009844:Oligosaccharides; D006820:Hyaluronic Acid; D002809:Chondroitin Sulfates