title: Immunohistochemical localization of superoxide dismutases in fetal and neonatal rat tissues.
abstract: We investigated the developmental profile of copper-zinc and manganese superoxide dismutase (CuZnSOD and MnSOD) in tissue sections obtained from fetal (Day 12 to 21 of gestation) and neonatal (Day 0 and 6) rats. Tissues were stained immunohistochemically with specific antisera against the respective rat SODs. There was a general trend towards richness of SODs in the epithelial linings and metabolically active sites, although differential distribution between the two SODs also existed. At Day 12 of gestation, immunoreactivity for both SODs was detected in the cardiomyocytes but not in other tissues. Hepatocytes expressed CuZnSOD at Day 14 and MnSOD at Day 17. By Day 18 CuZnSOD was detected in the epithelial cells of the gastrointestinal tract, respiratory tract, pancreatic islets, kidneys, and adrenals. These tissues exhibited MnSOD staining at Day 19. CuZnSOD occurred in the epithelia of the thyroid, thymus, and salivary glands at Day 19, while MnSOD was seen at Day 21. The increase in intensity of the staining for SODs occurred no later than postnatal Day 0, indicating that most tissues accumulated SODs during late gestation. Breathing atmospheric oxygen during early extrauterine life did not appreciably intensify the SOD staining. These results suggest that perinatal increase in SODs occurs as a general mechanism of preparation for birth.
authors.affiliation: Department of Pediatrics, Yamanashi Medical College, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Munim
authors.forename: K
authors.initials: K
authors.lastname: Asayama
authors.forename: K
authors.initials: K
authors.lastname: Dobashi
authors.forename: K
authors.initials: K
authors.lastname: Suzuki
authors.forename: A
authors.initials: A
authors.lastname: Kawaoi
authors.forename: K
authors.initials: K
authors.lastname: Kato
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D015153:Blotting, Western; D005260:Female; D005333:Fetus / Q000201:enzymology*; D007150:Immunohistochemistry; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000378:metabolism*; D014018:Tissue Distribution
chemical_list: D013482:Superoxide Dismutase