Studies of the molecular recognition of synthetic methyl beta-lactoside analogues by Ricinus communis agglutinin.
The 2-, 3-, 6-, 2'-, 3'-, 4'-, and 6'-deoxy derivatives and the 3-O-methyl derivative of methyl beta-lactoside have been synthesised and their binding to the galactose-specific agglutinin from Ricinus communis (RCA-120) has been investigated. The results indicate that HO-3,4,6 of the beta-D-galactopyranose moiety are the key polar groups. The main difference from the closely related ricin lectin RCA-60 involves HO-6 of the D-glucopyranose moiety, which seems to contribute to the binding of the carbohydrate to RCA-60 but not to RCA-120.
Rivera-Sagredo
A
A
Instituto de Química Orgánica, C.S.I.C., Madrid, Spain.
Jiménez-Barbero
J
J
Martín-Lomas
M
M
Solís
D
D
Díaz-Mauriño
T
T
D002240:Carbohydrate Sequence; D002367:Ricinus communis / Q000737:chemistry*; D007785:Lactose / Q000031:analogs & derivatives*; D037102:Lectins / Q000737:chemistry*; D046911:Macromolecular Substances; D008759:Methylglycosides / Q000737:chemistry*; D008969:Molecular Sequence Data; D037121:Plant Lectins; D010947:Plants, Toxic; D012995:Solubility
D037102:Lectins; D046911:Macromolecular Substances; D008759:Methylglycosides; D037121:Plant Lectins; C017707:Ricinus communis agglutinin-1; C034207:methyl lactoside; D007785:Lactose