title: New analogues of Cucurbita maxima trypsin inhibitor III (CMTI III) with simplified structure.
abstract: Seven new analogues of trypsin inhibitor CMTI III were obtained by solid-phase peptide synthesis. Three analogues contained only two, instead of three, disulfide bridges, whereas the molecules of the next four analogues were shortened at the N- and/or C-terminus. The elimination of one disulfide bridge in CMTI III induces a decrease in the association equilibrium constants by 6-7 orders of magnitude, whereas the removal of one, two or three amino-acid residues at the N- and/or C-terminus does not significantly affect the activity.
authors.affiliation: Faculty of Chemistry, University of Gdańsk, Poland.
authors.forename: K
authors.initials: K
authors.lastname: Rolka
authors.forename: G
authors.initials: G
authors.lastname: Kupryszewski
authors.forename: J
authors.initials: J
authors.lastname: Rózycki
authors.forename: U
authors.initials: U
authors.lastname: Ragnarsson
authors.forename: T
authors.initials: T
authors.lastname: Zbyryt
authors.forename: J
authors.initials: J
authors.lastname: Otlewski
mesh_terms: D000595:Amino Acid Sequence; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D014361:Trypsin Inhibitors / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology
chemical_list: C081870:CMTI protein, Cucurbita maxima; D010940:Plant Proteins; D014361:Trypsin Inhibitors