title: Incorporation of n-3 fatty acids of fish oil into tissue and serum lipids of ruminants.
abstract: This study examines the biohydrogenation and utilization of the C20 and C22 polyenoic fatty acids in ruminants. Eicosapentaenoic (20:5n-3) and docosahexaenoic (22:6n-3) acids were not biohydrogenated to any significant extent by rumen microorganisms, whereas C18 polyenoic fatty acids were extensively hydrogenated. The feeding of protected fish oil increased the proportion of 20:5 from 1% to 13-18% and 22:6 from 2% to 7-9% in serum lipids and there were reductions in the proportion of stearic (18:0) and linoleic (18:2) acids. The proportion of 20:5 in muscle phospholipids (PL) increased from 1.5% to 14.7% and 22:6 from 1.0% to 4.2%; these acids were not incorporated into muscle or adipose tissue triacylglycerols (TAG). In the total PL of muscle, the incorporated 20:5 and 22:6 substituted primarily for oleic (18:1) and/or linoleic (18:2) acid, and there was no consistent change in the porportion of arachidonic (20:4) acid.
authors.affiliation: CSIRO, Division of Animal Production, Prospect, N.S.W., Australia.
authors.forename: J R
authors.initials: JR
authors.lastname: Ashes
authors.forename: B D
authors.initials: BD
authors.lastname: Siebert
authors.forename: S K
authors.initials: SK
authors.lastname: Gulati
authors.forename: A Z
authors.initials: AZ
authors.lastname: Cuthbertson
authors.forename: T W
authors.initials: TW
authors.lastname: Scott
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism; D000818:Animals; D001419:Bacteria / Q000378:metabolism; D002417:Cattle / Q000378:metabolism*; D015525:Fatty Acids, Omega-3 / Q000097:blood / Q000378:metabolism*; D005395:Fish Oils / Q000378:metabolism*; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000378:metabolism; D050356:Lipid Metabolism; D008055:Lipids / Q000097:blood; D008066:Lipolysis; D009132:Muscles / Q000378:metabolism; D019301:Oleic Acid; D009829:Oleic Acids / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism; D010938:Plant Oils / Q000378:metabolism; D012417:Rumen / Q000382:microbiology; D012756:Sheep / Q000378:metabolism*; D013229:Stearic Acids / Q000378:metabolism; D000074242:Sunflower Oil; D014280:Triglycerides / Q000378:metabolism
chemical_list: D015525:Fatty Acids, Omega-3; D005395:Fish Oils; D008041:Linoleic Acids; D008055:Lipids; D009829:Oleic Acids; D010743:Phospholipids; D010938:Plant Oils; D013229:Stearic Acids; D000074242:Sunflower Oil; D014280:Triglycerides; D019301:Oleic Acid; C031183:stearic acid; D019787:Linoleic Acid