title: Determination of cystathionine and perhydro-1,4-thiazepine-3,5-dicarboxylic acid in the urine of a patient with cystathioninuria using column liquid chromatography-mass spectrometry.
abstract: A method for the measurement of cystathionine and perhydro-1,4-thiazepine-3,5-dicarboxylic acid in the urine of a patient with cystathioninuria has been developed, using column liquid chromatography-mass spectrometry. Cystathionine and perhydro-1,4-thiazepine-3,5-dicarboxylic acid were determined by scanning the [M + H]+ ions of each compound. The recoveries were 80-92.4% for cystathionine and 80-100% for perhydro-1,4-thiazepine-3,5-dicarboxylic acid after ion-exchange treatment. The results agreed well with those obtained using an amino acid analyser. The concentrations found for cystathionine and perhydro-1,4-thiazepine-3,5-dicarboxylic acid were 1.289 +/- 0.099 mg/ml and 0.310 +/- 0.0067 mg/ml, respectively.
authors.affiliation: Department of Chemistry, Kochi Medical School, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Sugahara
authors.forename: J
authors.initials: J
authors.lastname: Ohta
authors.forename: M
authors.initials: M
authors.lastname: Takemura
authors.forename: H
authors.initials: H
authors.lastname: Kodama
mesh_terms: D003540:Cystathionine / Q000652:urine*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D013841:Thiazepines / Q000652:urine*
chemical_list: D013841:Thiazepines; D003540:Cystathionine; C010280:perhydro-1,4-thiazepine-3,5-dicarboxylic acid