title: Development of a direct and indirect chemiluminescence immunoassay for the detection of an organophosphorus compound.
abstract: The following study describes the development of two immunoassays for the semiquantitative determination of the organophosphorus compound methyl phosphonic acid, p-aminophenyl 1,2,2-trimethyl-propyl diester (MATP). These assays are a direct competitive, labelled-hapten, enzyme immunoassay and a competitive enzyme immunoassay with indirectly labelled antibody, both with luminescence determination of peroxidase. A camera luminometer was used to determine final signal strength. A constant light reaction was reached using luminol (5-amino-2,3-dihydro-1,4-phalazinedione), and by adding coumaric acid as an enhancer. A five minute interval was selected as exposure time. This phototechnical system makes a semiquantitative detection of MATP possible. The detection limits for both assays were at 10(-6)mol/l MATP. With the help of a microtiter luminometer (reader) the detection limit was reduced to 5 x 10(-8) mol/l MATP, by measuring the real intensity of the chemiluminescence signal.
authors.affiliation: Institut für Tierphysiologie, Tierärztliche Fakultät, Universität München, Federal Republic of Germany.
authors.forename: M H
authors.initials: MH
authors.lastname: Erhard
authors.forename: A
authors.initials: A
authors.lastname: Jüngling
authors.forename: S
authors.initials: S
authors.lastname: Brendgen
authors.forename: J
authors.initials: J
authors.lastname: Kellner
authors.forename: U
authors.initials: U
authors.lastname: Lösch
mesh_terms: D000911:Antibodies, Monoclonal; D001667:Binding, Competitive; D005069:Evaluation Studies as Topic; D006241:Haptens; D007118:Immunoassay / Q000379:methods* / Q000706:statistics & numerical data; D008163:Luminescent Measurements; D009943:Organophosphorus Compounds / Q000032:analysis* / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D006241:Haptens; D009943:Organophosphorus Compounds; C056499:4-aminophenylphosphonic acid methyl-1,2,2-trimethylpropyl diester