Antioxidant defenses in the bile duct-ligated rat.
Changes in pro-oxidant and antioxidant balance in the serum and liver were studied in an experimental model of obstructive jaundice in the rat. The results showed a decrease in plasma vitamin E concentration (P < 0.01) and a threefold reduction in liver vitamin E concentration (P < 0.001). There was also a threefold reduction in levels of the liver enzymes glutathione peroxidase (P < 0.01) and glutathione transferase (P < 0.001), together with a six-fold reduction in catalase activity (P < 0.001). The serum selenium level decreased by 35% in the jaundiced rats (P < 0.05). The total liver glutathione level decreased to half the control value (P < 0.01). The malonyldialdehyde level, the measure of lipid peroxidation used in this study, doubled (P < 0.01). The results suggest a shift in the pro-oxidant/antioxidant balance in favor of lipid peroxidation. The possible etiology of this change and its relationship to human cholestasis are discussed.
Singh
S
S
Robens Institute, University of Surrey, Guildford, England.
Shackleton
G
G
Ah-Sing
E
E
Chakraborty
J
J
Bailey
M E
ME
D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D001663:Bilirubin / Q000097:blood; D002374:Catalase / Q000378:metabolism; D001651:Cholestasis, Extrahepatic / Q000378:metabolism*; D004195:Disease Models, Animal; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D015227:Lipid Peroxidation; D008099:Liver / Q000201:enzymology; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D010084:Oxidation-Reduction; D051381:Rats; D017208:Rats, Wistar; D012643:Selenium / Q000097:blood; D014810:Vitamin E / Q000494:pharmacology*
D000975:Antioxidants; D014810:Vitamin E; D008315:Malondialdehyde; D002374:Catalase; D005979:Glutathione Peroxidase; D005982:Glutathione Transferase; D005978:Glutathione; D012643:Selenium; D001663:Bilirubin