[Elucidation on abnormal behavior of glycyrrhizin salts in high performance liquid chromatography determination for quasi-drug products].
Glycyrrhizinates such as monoammonium and dipotassium glycyrrhizinates which are extracted from licorice, converted to easily water-soluble salts and refined have been formulated in many quasi-drug products as an anti-inflammatory agent. A quantitative analysis of glycyrrhizinates in quasi-drug products has usually and successfully been carried out by high performance liquid chromatography (HPLC). However, a slightly higher value than the actual content has sometimes been observed in daily analysis. As the result of our various studies to investigate these phenomena, we finally found that a minor constituent in commercial glycyrrhizinates, which was identified as monomethyl glycyrrhizinate, was hydrolyzed after they were formulated in quasi-drug products and converted to glycyrrhizin which in turn was determined by HPLC as a higher value.
Hayashi
M
M
Analytical Research Group, Safety & Analytical Research Center, Shiseido Research & Development Headquarters, Yokohama, Japan.
Kadowaki
E
E
Takamatsu
T
T
Matsuoka
M
M
D002851:Chromatography, High Pressure Liquid; D006034:Glycyrrhetinic Acid / Q000031:analogs & derivatives* / Q000032:analysis; D019695:Glycyrrhizic Acid; D006863:Hydrogen-Ion Concentration
D019695:Glycyrrhizic Acid; D006034:Glycyrrhetinic Acid