Transfer of specific immunity from donor to recipient of an allogeneic bone marrow graft: evidence for donor origin of the antibody producing cells.
A rapid recovery of specific humoral immunity in the recipient of an allogeneic bone marrow transplantation (BMT) can be observed after immunization of the donor before graft sampling. This has been attributed to transfer of specific immunity from donor to recipient. However, to maintain the concept of transfer the origin of the antibody producing cells in the recipient after BMT must be demonstrated. To this end, donor-recipient pairs with differences in Gm-allotypes were selected and immunized before BMT with the neo-antigen Helix pomatia haemocyanin (HPH) according to three immunization protocols. Additionally, the recipients were immunized at day 42 after BMT. Serum samples were weekly obtained from the recipients in the first 100 d after BMT. The origin of HPH-specific antibody producing cells was assessed by two approaches: (1) determination of the Gm-allotypes of anti-HPH antibodies within a distinct IgG subclass, (2) analysis of anti-HPH antibody spectrotypes by isoelectric focusing combined with immunoblotting. The results obtained with these two approaches show concordance in most instances and led to the conclusion that the antibody producing cells are of donor origin.
Labadie
J
J
Department of Paediatrics, Leiden University Hospital, The Netherlands.
van Tol
M J
MJ
Dijkstra
N H
NH
van der Kaaden
M
M
Jol-van der Zijde
C M
CM
de Lange
G G
GG
Zwaan
F E
FE
Vossen
J M
JM
D000328:Adult; D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis*; D000917:Antibody Formation; D016026:Bone Marrow Transplantation / Q000276:immunology*; D002648:Child; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006372:Helix, Snails / Q000276:immunology*; D006433:Hemocyanins / Q000276:immunology; D006801:Humans; D015151:Immunoblotting; D007074:Immunoglobulin G / Q000032:analysis; D007129:Immunoglobulin Gm Allotypes / Q000032:analysis; D007525:Isoelectric Focusing; D008297:Male; D010641:Phenotype; D011446:Prospective Studies; D011897:Random Allocation; D013745:Tetanus Toxoid / Q000276:immunology*; D014019:Tissue Donors
D000907:Antibodies, Bacterial; D007074:Immunoglobulin G; D007129:Immunoglobulin Gm Allotypes; D013745:Tetanus Toxoid; D006433:Hemocyanins