A reversed activity staining procedure for detection of an acquired antibody against factor XIII in a girl with factor XIII deficiency.
A new method for detection of inhibitors against factor XIII is described. Agarose gel electrophoresis with normal platelet poor plasma as a factor XIII source included in the gel was combined with reversed activity staining. The procedure was applied to a 10-year-old girl with factor XIII deficiency where substitution therapy had failed. Plasma samples from the patient inhibited the factor XIII catalysed incorporation of the fluorescent monodansylthiacadaverine into casein at a position corresponding to the migration of gamma-globulins. Treatment with Sepharose-bound protein A effectively adsorbed the factor XIII antibody in the patient's plasma.
Seiving
B
B
Hospital Pharmacy, Malmö General Hospital, Sweden.
Henriksson
P
P
Stenberg
P
P
Nilsson
I M
IM
D000906:Antibodies / Q000032:analysis*; D002103:Cadaverine / Q000031:analogs & derivatives; D002648:Child; D004587:Electrophoresis, Agar Gel; D005176:Factor XIII / Q000276:immunology*; D005177:Factor XIII Deficiency / Q000276:immunology*; D005260:Female; D005456:Fluorescent Dyes; D006801:Humans; D007158:Immunologic Techniques
D000906:Antibodies; D005456:Fluorescent Dyes; D005176:Factor XIII; C008542:monodansylcadaverine; D002103:Cadaverine