[Measurement of specific IgE (sIgE) using the MATRIX technique].
In this study, on 34 subjects, the technique for measurement of sIgE by the MATRIX technique has been compared with the reference of CAP RAST, skin tests and clinical history. The two techniques of CAP RAST and MATRIX are judged to be comparable (correlation percentage 88.2%). The results obtained with four allergens [D. pteronyssinus, Timothy grass (Phleum pratense), cat and dog] done separately confirm the reliability of the technique. The study was completed by a comparison of the advantage and disadvantages of CAP RAST and MATRIX. This shows that the manipulation time is much reduced, but with a longer delay time and high reagent cost. In contrast, it has the enormous advantage of using a standardised range of allergens.
Sabbah
A
A
Laboratoire d'Immuno-allergologie, CHU, Angers.
Biteau
Y
Y
Lauret
M G
MG
D000293:Adolescent; D000328:Adult; D000485:Allergens; D000918:Antibody Specificity; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000175:diagnosis / Q000276:immunology; D007124:Immunoenzyme Techniques / Q000191:economics; D007073:Immunoglobulin E / Q000097:blood*; D007428:Intradermal Tests; D008297:Male; D008875:Middle Aged; D011237:Predictive Value of Tests; D011852:Radioallergosorbent Test / Q000191:economics; D011933:Reagent Kits, Diagnostic; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000485:Allergens; D011933:Reagent Kits, Diagnostic; D007073:Immunoglobulin E
Dosage des IgE spécifiques (sIgE) par la technique MATRIX.