The same few V genes account for a majority of oxazolone antibodies in most mouse strains.
The early primary anti-phenyloxazolone antibodies of 12 mouse strains were studied by determining proportions of two defined subsets id495 (the classical phOx idiotype) and id350. Id495-positive antibodies bear an H chain encoded by VHOx1 gene (family Q52) and an L chain usually coded for by VKOx1 but occasionally by other VK genes. Id350-positive antibodies are encoded by a VK gene VK45.1, and usually by a VH gene of the S107 family. All 12 strains (representing nine H-chain and four kappa-chain haplotypes) produced id350-positive anti-phOx antibodies. While id495 is the predominant major subset in the BALB/c response (originally studied), id350 seems to be the predominant subset of early anti-phOx antibodies in the mouse species. The combined proportion of the two subsets varied from ca. 50 to almost 100% of the total in all strains except C57BL.
Solin
M L
ML
Department of Bacteriology and Immunology, University of Helsinki, Finland.
Kaartinen
M
M
Mäkelä
O
O
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000032:analysis / Q000235:genetics*; D000911:Antibodies, Monoclonal; D000917:Antibody Formation; D001483:Base Sequence; D005803:Genes, Immunoglobulin; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D007130:Immunoglobulin Idiotypes / Q000235:genetics; D007135:Immunoglobulin Variable Region / Q000235:genetics*; D051379:Mice; D008815:Mice, Inbred Strains / Q000276:immunology*; D008969:Molecular Sequence Data; D010081:Oxazolone / Q000276:immunology*; D012333:RNA, Messenger / Q000235:genetics; D011863:Radioimmunoassay; D012689:Sequence Homology, Nucleic Acid
D000906:Antibodies; D000911:Antibodies, Monoclonal; D007143:Immunoglobulin Heavy Chains; D007130:Immunoglobulin Idiotypes; D007135:Immunoglobulin Variable Region; D012333:RNA, Messenger; D010081:Oxazolone