title: Detection of intracellular interleukin-10 by flow cytometry.
abstract: Detection of cytokines is limited to measurements of the secreted molecule. To circumvent this problem we permeabilized the cell membrane with digitonin and localize cytokine expression using antibodies by flow cytometry. In this report we demonstrate that we can detect specific intracellular interleukin-10 (IL-10) in the HT-2 T cell line only after membrane permeabilization. With this technique intracellular cytokines are readily detectable.
authors.affiliation: Brigham and Women's Hospital, Department of Medicine, Boston, MA 02115.
authors.forename: B
authors.initials: B
authors.lastname: Kreft
authors.forename: G G
authors.initials: GG
authors.lastname: Singer
authors.forename: C
authors.initials: C
authors.lastname: Diaz-Gallo
authors.forename: V R
authors.initials: VR
authors.lastname: Kelley
mesh_terms: D000818:Animals; D002463:Cell Membrane Permeability; D003593:Cytoplasm / Q000737:chemistry*; D005434:Flow Cytometry / Q000379:methods*; D066298:In Vitro Techniques; D016753:Interleukin-10 / Q000032:analysis*; D051379:Mice
chemical_list: D016753:Interleukin-10