Dissociation of store release from transmembrane influx of calcium in human neutrophils.
Release of Ca2+ from intracellular stores was visualised in individual neutrophils in the presence of the Mn2+ or SKF 96365. Influx of Mn2+ quenched fura-2 close to the plasma membrane but did not quench fura-2 at the site of store release. The size and location of the 'cloud' of elevated Ca2+ was unaffected by the channel blocker SKF 96365. Furthermore, the size and location was unaffected by the presence of extracellular Ca2+. This dissociation of transmembrane influx from store release demonstrates that the entry of Ca2+ into the cytosol of neutrophils occurs directly into the cytosol and not via the store site.
Davies
E V
EV
Department of Surgery, University of Wales College of Medicine, Cardiff, UK.
Campbell
A K
AK
Hallett
M B
MB
D002118:Calcium / Q000378:metabolism*; D006801:Humans; D007093:Imidazoles / Q000494:pharmacology; D066298:In Vitro Techniques; D008345:Manganese / Q000378:metabolism; D009504:Neutrophils / Q000378:metabolism*
D007093:Imidazoles; D008345:Manganese; C063159:1-(2-(3-(4-methoxyphenyl)propoxy)-4-methoxyphenylethyl)-1H-imidazole; D002118:Calcium