m-iodobenzylguanidine increases the mitochondrial Ca2+ pool in isolated hepatocytes.
The incubation of isolated hepatocytes with the inhibitor of protein mono ADP-ribosylation, m-iodobenzylguanidine (MIBG), resulted in an increase in the size of the mitochondrial Ca2+ pool, without alteration of the non-mitochondrial Ca2+ store(s). This increase was abolished when the cytosolic free Ca2+ concentration ([Ca2+]i) was buffered by prior loading of the cells with fluo 3. Elevating [Ca2+]i by releasing the endoplasmic reticular Ca2+ store with 2,5-di-(tert-butyl)-1,4-hydroquinone resulted in a synergistic increase in the magnitude of the mitochondrial Ca2+ pool. A role for protein ADP-ribosylation in the intracellular regulation of mitochondrial Ca2+ homeostasis is suggested.
Juedes
M J
MJ
Department of Toxicology, Karolinska Institutet, Stockholm, Sweden.
Kass
G E
GE
Orrenius
S
S
D019797:3-Iodobenzylguanidine; D000246:Adenosine Diphosphate Ribose / Q000378:metabolism; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002118:Calcium / Q000378:metabolism*; D002413:Cations, Divalent; D007462:Iodobenzenes / Q000494:pharmacology*; D008297:Male; D008930:Mitochondria, Liver / Q000187:drug effects* / Q000378:metabolism; D011506:Proteins / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
D002413:Cations, Divalent; D007462:Iodobenzenes; D011506:Proteins; D000246:Adenosine Diphosphate Ribose; D019797:3-Iodobenzylguanidine; D002118:Calcium