NaCl transport by Madin Darby canine kidney cyst epithelial cells.
The mechanism of NaCl transport across the epithelium of intact MDCK cysts grown in a collagen gel matrix was investigated. Double-barreled microelectrodes were used to measure basolateral membrane PD (Vbl), transepithelial PD (Vt), and intracellular (Cli) and intralumenal (Clcy) Cl- activities in cysts under different conditions. In a control Ringer's solution (RS), Cli (60 +/- 1 mM) and Clcy (107 +/- 2 mM) exceeded the values corresponding to electrochemical equilibrium across the basolateral membrane and epithelium, respectively. Cli was reduced by superfusing the cysts with a low Cl- RS (Cli, 20 +/- 3 mM), a low Na+ RS (Cli, 40 +/- 4 mM), or by adding amiloride to the control RS (Cli, 46 +/- 1 mM). Cli was unaffected by removal of either K+ or HCO3- from the RS or by adding furosemide or SITS to the control RS. Vbl in the control RS was -50 +/- 2 mV and was affected only by removal from the RS of K+ (Vbl, -31 +/- 3 mV) or HCO3- (Vbl, -29 +/- 4 mV) or by the addition of SITS to the control RS (Vbl, -59 +/- 5 mV). Vt in control RS was -2 +/- 0.2 mV (lumen negative), and was increased by reducing bath Na+ (Vt, -37 +/- 2 mV) but not by reducing bath Cl-. These data indicate that Cl- is secreted in a basolateral to apical direction by the cyst epithelium. Basolateral Cl- transport probably occurs mainly by an electroneutral Cl-/HCO3- exchanger. Transepithelial Na+ transport seems to occur via a paracellular route which appears to be cation selective. These experiments also support the existence, in the basolateral membrane, of a Na+/K+ ATPase, a Na+/H+ exchanger, and possibly a Na+/HCO3-/CO3(2-) transporter.
Macias
W L
WL
Department of Medicine, Indiana University School of Medicine, Indianapolis.
McAteer
J A
JA
Tanner
G A
GA
Fritz
A L
AL
Armstrong
W M
WM
D000818:Animals; D002460:Cell Line; D002712:Chlorides / Q000378:metabolism; D004285:Dogs; D004563:Electrochemistry; D004848:Epithelium / Q000378:metabolism; D017136:Ion Transport; D052177:Kidney Diseases, Cystic / Q000378:metabolism*; D008839:Microelectrodes; D012964:Sodium / Q000378:metabolism; D012965:Sodium Chloride / Q000378:metabolism*
D002712:Chlorides; D012965:Sodium Chloride; D012964:Sodium