A simple and rapid method for screening transformant yeast colonies using PCR.
A simple and rapid procedure for screening transformant yeast colonies is described. In this method, a trace amount of plasmid DNA is isolated from a small amount of yeast cell mass; then, the presence of the exogenous DNA in each yeast colony is detected by PCR amplification.
Liang
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Q
Department of Food Science and Technology, University of California, Davis 95616.
Richardson
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T
D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods; D004274:DNA, Recombinant / Q000032:analysis*; D004587:Electrophoresis, Agar Gel; D006801:Humans; D007781:Lactoferrin / Q000235:genetics; D008969:Molecular Sequence Data; D010957:Plasmids; D016133:Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D014170:Transformation, Genetic
D004274:DNA, Recombinant; D007781:Lactoferrin