Determination of r DNA hirudin and A-human thrombin- hirudin complex in plasma samples: enzyme linked immunosorbent assays for hirudin and complex vs. chromogenic thrombin substrate assay.
rDNA hirudin plasma concentrations in man and rhesus monkeys were determined over a period of 15 and 24 h. The plasma concentration of alpha-human thrombin-hirudin complex was measured after administration of the complex to rhesus monkeys. The complex was also determined after administration of hirudin to man and rhesus monkeys to study a possible formation of a complex with alpha-human thrombin in blood. The determination of hirudin was performed by a sandwich ELISA, using polyclonal and monoclonal antibodies and the chromogenic thrombin substrate assay. The alpha-human thrombin-hirudin complex concentration in the plasma of rhesus monkeys was measured over a period of 48 hours. The results of a sandwich ELISA were compared with those of the chromogenic thrombin substrate assay. A good agreement between the total hirudin concentrations analyzed by the hirudin ELISA and the alpha-human thrombin-hirudin complex ELISA and those of the chromogenic thrombin substrate assay, measuring total hirudin, too, was observed.
Berscheid
G
G
Hoechst AG, Frankfurt am Main, FRG.
Grötsch
H
H
Neubauer
H
H
Pünter
J
J
Reindl
J
J
Seipp
P
P
D000818:Animals; D002863:Chromogenic Compounds / Q000032:analysis*; D004275:DNA, Ribosomal / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay; D006629:Hirudins / Q000097:blood* / Q000737:chemistry; D006801:Humans; D008253:Macaca mulatta; D013917:Thrombin / Q000032:analysis* / Q000737:chemistry
D002863:Chromogenic Compounds; D004275:DNA, Ribosomal; D006629:Hirudins; D013917:Thrombin