Arterial thrombosis model with photochemical reaction in guinea-pig and its property.
We have already developed an arterial thrombosis model in the rat femoral artery which utilized photochemical reaction between systemically injected rose bengal and transillumination of a green light with 540 nm wave length from the outside of the vessel. In the present study, we applied this model to guinea-pigs in order to produce a more suitable thrombus model for evaluation of antithrombotic drugs which act on the prostaglandin cascade. In the guinea-pigs, the irradiated femoral artery was completely occluded in 7 min after the injection of rose bengal (10 mg/kg) in a similar manner to the rats. The processes of primary endothelial injury and the subsequent formation of thrombus during this manipulation were observed by the electron microscopy. Pretreatment with aspirin and Y-20811, a thromboxane synthetase inhibitor, significantly prolonged the time required for occlusion in the guinea-pigs, while these drugs were ineffective in the rats. The antithrombotic effect of vapiprost, a thromboxane A2 receptor antagonist, was more pronounced in the guinea-pigs than the rats. In conclusion, this model in guinea-pigs is more suitable for evaluating antithrombotic drugs, particularly, the action of which is exerted involving the prostaglandin cascade.
Takiguchi
Y
Y
Department of Pharmacology, Hamamatsu University School of Medicine, Japan.
Hirata
Y
Y
Wada
K
K
Nakashima
M
M
D000818:Animals; D001241:Aspirin / Q000494:pharmacology; D001713:Biphenyl Compounds / Q000627:therapeutic use; D004195:Disease Models, Animal; D005263:Femoral Artery; D005343:Fibrinolytic Agents / Q000627:therapeutic use; D006168:Guinea Pigs / Q000097:blood*; D006538:Heptanoic Acids / Q000627:therapeutic use; D007093:Imidazoles / Q000494:pharmacology; D008027:Light; D008297:Male; D008855:Microscopy, Electron, Scanning; D010777:Photochemistry; D010974:Platelet Aggregation / Q000187:drug effects; D011453:Prostaglandins / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D012395:Rose Bengal / Q000528:radiation effects / Q000633:toxicity*; D013927:Thrombosis / Q000139:chemically induced* / Q000188:drug therapy
D001713:Biphenyl Compounds; D005343:Fibrinolytic Agents; D006538:Heptanoic Acids; D007093:Imidazoles; D011453:Prostaglandins; D012395:Rose Bengal; C049966:Y 20811; C060013:vapiprost; D001241:Aspirin