title: Molecular cloning and developmental expression of a human kidney S-adenosylmethionine synthetase.
abstract: cDNA clones encoding the human kidney S-adenosylmethionine synthetase (kidney-type isozyme) were isolated. The amino acid sequence deduced from the cDNA indicates that this enzyme contains 395 amino acids and has a molecular mass of 43,660 Da. The predicted amino acid sequence of this protein shares 84% similarity with that of human liver S-adenosylmethionine synthetase (liver-type isozyme). In addition, the developmental expression of these two isozyme mRNAs has been studied in the human liver using the reverse transcription-polymerase chain reaction (RT-PCR).
authors.affiliation: Department of Pathological Biochemistry, Tokyo Medical and Dental University, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Horikawa
authors.forename: K
authors.initials: K
authors.lastname: Tsukada
mesh_terms: D000328:Adult; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D005333:Fetus; D006801:Humans; D007527:Isoenzymes / Q000235:genetics*; D007668:Kidney / Q000196:embryology / Q000201:enzymology*; D008099:Liver / Q000201:enzymology; D008716:Methionine Adenosyltransferase / Q000235:genetics*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017386:Sequence Homology, Amino Acid
chemical_list: D007527:Isoenzymes; D012333:RNA, Messenger; D004247:DNA; D008716:Methionine Adenosyltransferase