[The effect of the antioxidant ionol as a liposome component on the function of the isolated and ischemic heart].
The anti-ischemic and toxic effects of different doses of the antioxidant ionol (butyl hydroxytoluene, BHT) introduced into the Krebs-Henseleit medium composed of monolayer liposomes of egg phospholipids before or 30 minutes after total ischemia of the heart were studied on a model of perfusion of isolated Wistar rat heart by the method of Langendorff-Fallen. It has been demonstrated that ionol, after its addition to the perfusate in the preischemic period, exerts an anti-ischemic effect in concentrations of 10(-6), 3 x 10(-6) and 10(-5) M; in the postischemic period, in concentrations of 3 x 10(-6) and 10(-5); the protective effect of ionol in the postischemic period is less pronounced. In higher doses (3 x 10(-5) and 10(-4) M) ionol produces a toxic action which is more remarkable and is less reversible in respect to the ischemized than to the nonischemized heart, and is realized rather through the dysfunction of heart muscle relaxation. The conclusion is drawn that there is a possibility of using ionol in doses of 10(-6) to 10(-5) M as a constituent of liposomes for addition to perfusion, conservant and cardioplegic solutions as an anti-ischemic remedy.
Morgunov
A A
AA
Bulgakov
V G
VG
Bilenko
M V
MV
D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage* / Q000627:therapeutic use / Q000633:toxicity; D002084:Butylated Hydroxytoluene / Q000008:administration & dosage* / Q000627:therapeutic use / Q000633:toxicity; D004305:Dose-Response Relationship, Drug; D004337:Drug Carriers; D004353:Drug Evaluation, Preclinical; D006321:Heart / Q000187:drug effects* / Q000502:physiology; D066298:In Vitro Techniques; D008081:Liposomes; D008297:Male; D017202:Myocardial Ischemia / Q000188:drug therapy* / Q000503:physiopathology; D010477:Perfusion; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
D000975:Antioxidants; D004337:Drug Carriers; D008081:Liposomes; D002084:Butylated Hydroxytoluene
Vliianie antioksidanta ionola v sostav liposom na funktsiiu izolirovannogo i ishemizirovannogo serdtsa.