title: Analysis of nuclear 3,3',5-triiodothyronine receptor in the brown adipose tissue (BAT) of the postnatal lamb.
abstract: Postnatal thermogenesis in sheep is associated with increased sympathoadrenal activities, a T3 surge and an enhanced brown adipose tissue (BAT) type II 5'-monodeiodinating (5'-MDI) activity. The latter peaks 3-4 days after birth and is known to be important in generating intracellular T3 for nuclear receptor binding. In order to further investigate the mechanism(s) responsible for neonatal thermogenesis, thyroid hormone nuclear receptor (T3NR) binding characteristics were quantified in lamb BAT from newborn (NB) to 30d of postnatal age. Maximal binding capacities (MBC, mean +/- SEM fmoles T3/mg DNA) in BAT showed a decrease as studied by ANOVA during the first 11 days (NB to 1d, 148 +/- 24 [N = 5, p < 0.01, cf. 3-5d group]; 3-5 d, 61 +/- 5.5 [N = 5]; 10-11d, 72 +/- 9.1 [N = 4]). Afterwards, MBC increased at 30d (196 +/- 32, N = 4, p less than 0.01, cf. 3-5d group). BAT T3NR binding affinities (10(9) M-1) were comparable in all age groups studied (NB-1d, 2.8 +/- 0.3; 3-5d, 3.4 +/- 0.3; 10-11d, 4.0 +/- 1.1; 30d, 2.4 +/- 0.4). The data suggest that the postnatal surge in T3 and type II 5'-MDI is accompanied with a concurrent decrease in MBC of BAT T3NR. The latter may represent a down-regulation of T3NR presumably in an attempt to regulate the overall effect of thyroid hormone in neonatal thermogenesis.
authors.affiliation: Nuclear Medicine Service, Veterans Administration Medical Center, Long Beach, California.
authors.forename: S Y
authors.initials: SY
authors.lastname: Wu
authors.forename: J K
authors.initials: JK
authors.lastname: Kim
authors.forename: D T
authors.initials: DT
authors.lastname: Tjioe
authors.forename: W S
authors.initials: WS
authors.lastname: Huang
authors.forename: I J
authors.initials: IJ
authors.lastname: Chopra
mesh_terms: D002001:Adipose Tissue, Brown / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism*; D003080:Cold Temperature; D004247:DNA / Q000378:metabolism; D011506:Proteins / Q000378:metabolism; D011988:Receptors, Thyroid Hormone / Q000378:metabolism*; D012756:Sheep; D013974:Thyroxine / Q000378:metabolism; D014284:Triiodothyronine / Q000378:metabolism
chemical_list: D011506:Proteins; D011988:Receptors, Thyroid Hormone; D014284:Triiodothyronine; D004247:DNA; D013974:Thyroxine