Cloning and sequencing of a plasmid-mediated erythromycin resistance determinant from Staphylococcus xylosus.
A 2.3-kb DNA fragment cloned from plasmid pCH200, the largest (52 kb) of four plasmids detected in Staphylococcus xylosus, was found to confer resistance to 14-membered ring macrolides in Bacillus subtilis and Staphylococcus aureus. DNA-sequence analysis of the fragment revealed the presence of an open-reading frame, the deduced product of which was identical to one of the two ATP-binding domains encoded by the macrolide/streptogramin-B-resistance gene msrA of Staphylococcus epidermidis. The observation that a polypeptide homologous to the C-terminus of MsrA is capable of mediating erythromycin resistance in the absence of the N-terminal region is of significance both to the evolution and functional activity of members of the ATP-binding transport super-gene family.
Milton
I D
ID
Department of Microbiology, Medical School, Newcastle upon Tyne, UK.
Hewitt
C L
CL
Harwood
C R
CR
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D004352:Drug Resistance, Microbial / Q000235:genetics*; D004917:Erythromycin / Q000494:pharmacology; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D010957:Plasmids; D013210:Staphylococcus / Q000187:drug effects / Q000235:genetics*
D004269:DNA, Bacterial; D004917:Erythromycin