title: Effect of chemical sympathectomy on scintigraphic gastric and small bowel transit in the rat.
abstract: Our aims were to measure gastric and small bowel transit of radiolabeled chow or liquids in rats and to assess the effect of pharmacologically induced chronic sympathectomy of at least 5 weeks duration. Three series of experiments were performed. In series I, four rats in each group underwent i.p. chloral hydrate anesthesia, tracheostomy with intubation and mechanical ventilation: they received by gavage 1.0 g rat chow dissolved in 1.5 ml saline labeled with 1.0 mCi 99mTc-DTPA In series II, four rats in each group were anesthetized with only i.p. chloral hydrate and received 1.5 ml labeled saline. Rats were placed on a gamma camera for dynamic acquisition of 1-min images for 7 h in series I, and 4 h in series II. Activity was quantitated in stomach and cecum; gastric emptying was analyzed by the power exponential model. In series III, four rats in each group were studied for 1 h on gamma camera and sacrificed after 5 h. At the end of each study, the stomach, small bowel, cecum and colon were removed and radioactivity counted in each organ in a dose calibrator. Quantitation of radioactivity by gamma camera was highly accurate: labeled chow in the stomach at 7 h was identical by external camera and counting of the excised organ (linear regression slope = 1.01, r = 0.97, P less than 0.0001). Similar precision was observed with labeled liquids (slope = 0.93, r = 0.93, P = 0.001). Chow emptied slower than liquid in both groups (P = 0.048). Ileocecal movement of isotope occurred by bolus transfers.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Gastroenterology Research Unit, Mayo Clinic, Rochester, Minnesota 55905.
authors.forename: I
authors.initials: I
authors.lastname: Ruytjens
authors.forename: G M
authors.initials: GM
authors.lastname: Thomforde
authors.forename: M
authors.initials: M
authors.lastname: Camilleri
authors.forename: N J
authors.initials: NJ
authors.lastname: Chapman
mesh_terms: D000818:Animals; D005746:Gastric Emptying / Q000187:drug effects*; D005766:Gastrointestinal Contents / Q000000981:diagnostic imaging*; D005769:Gastrointestinal Motility / Q000187:drug effects* / Q000502:physiology; D005772:Gastrointestinal Transit / Q000187:drug effects* / Q000502:physiology; D006145:Guanethidine / Q000494:pharmacology*; D008297:Male; D011877:Radionuclide Imaging; D051381:Rats; D017207:Rats, Sprague-Dawley; D013563:Sympathectomy, Chemical; D016284:Technetium Tc 99m Pentetate
chemical_list: D016284:Technetium Tc 99m Pentetate; D006145:Guanethidine