title: Pseudomonas aeruginosa biofilm as a diffusion barrier to piperacillin.
abstract: Pseudomonas aeruginosa 579 biofilms formed on dialysis membranes retarded piperacillin diffusion. Treatment of biofilms with 5.0 mM CaCl2.2H2O prevented diffusion. Biofilms permitted equilibration of [14C]glucose. Thin-layer chromatography of fluids distal to untreated (viable and nonviable) and viable Ca(2+)-treated P. aeruginosa 579 biofilms and fluids distal to a viable P. aeruginosa mutant noninducible for the expression of beta-lactamase did not detect piperacillinoic acid.
authors.affiliation: Department of Biological Sciences, University of Calgary, Alberta, Canada.
authors.forename: B D
authors.initials: BD
authors.lastname: Hoyle
authors.forename: J
authors.initials: J
authors.lastname: Alcantara
authors.forename: J W
authors.initials: JW
authors.lastname: Costerton
mesh_terms: D002855:Chromatography, Thin Layer; D004058:Diffusion; D006868:Hydrolysis; D008566:Membranes / Q000737:chemistry; D010878:Piperacillin / Q000737:chemistry*; D011550:Pseudomonas aeruginosa / Q000737:chemistry* / Q000378:metabolism
chemical_list: D010878:Piperacillin