Calcium ionophore A-23187 inhibits the secretion of beta-hexosaminidase from the GG2EE mouse macrophage cell line.
Secretion of the lysosomal enzyme beta-N-acetylhexosaminidase is inhibited by calcium ionophore A-23187 in the GG2EE macrophage cell line. Such inhibition is time and dose dependent. Calcium ionophore A-23187 treatment causes a change in the pattern of hexosaminidase isoenzymes detectable in the cell extract, as assessed by DEAE-cellulose chromatography. In particular, control cells show two hexosaminidase isoenzymes corresponding to hexosaminidase A and B, whereas cells treated with calcium ionophore A-23187 express a third isoenzyme form with properties similar to hexosaminidase S.
Beccari
T
T
Dipartimento di Medicina Sperimentale e Scienze Biochemiche, Università di Perugia, Italia.
Datti
A
A
Orlacchio
A
A
Farinelli
S
S
Blasi
E
E
D000818:Animals; D000001:Calcimycin / Q000494:pharmacology*; D002460:Cell Line; D002848:Chromatography, DEAE-Cellulose; D054818:Hexosaminidase A; D007527:Isoenzymes / Q000187:drug effects* / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects* / Q000201:enzymology; D051379:Mice; D001619:beta-N-Acetylhexosaminidases / Q000187:drug effects* / Q000378:metabolism
D007527:Isoenzymes; D000001:Calcimycin; D054818:Hexosaminidase A; D001619:beta-N-Acetylhexosaminidases