Suppurative granulomatous lymphadenitis. Immunohistochemical evidence for a B-cell-associated granuloma.
The cellular composition of suppurative granulomas was investigated by the application of monoclonal and polyclonal antibodies to paraffin sections and compared with nonsuppurative, hypersensitivity-type granulomas. Macrophages, T cells, and dendritic cells showed a similar distribution in both types of granulomas. In addition to the presence of granulocytes, a major difference between suppurative granulomas and hypersensitive-type granulomas concerned their relationship with B lymphocytes. Hypersensitive-type granulomas were surrounded by small mantle B cells, but they did not contain any B lymphocytes. In contrast, variable numbers of B cells were found either at the periphery or in the center of suppurative granulomas. In view of their morphology (medium size, pale cytoplasm, irregular nuclear shape) and phenotype (L26 +/LN1 -/MB2 +/MT2 +) these B lymphocytes closely resembled monocytoid B cells. The monocytoid B cells might have a role in the recruitment of polymorphonuclear granulocytes and in the development of the necrosis, which occur within suppurative granulomas.
Facchetti
F
F
Department of Pathology, University of Brescia, Italy.
Agostini
C
C
Chilosi
M
M
Mombello
A
A
Grigolato
P
P
Van den Oord
J J
JJ
D000135:Acid Phosphatase / Q000032:analysis; D000469:Alkaline Phosphatase / Q000032:analysis; D000911:Antibodies, Monoclonal / Q000032:analysis; D001402:B-Lymphocytes / Q000737:chemistry / Q000473:pathology*; D001706:Biopsy; D002272:Carcinoembryonic Antigen / Q000032:analysis; D002467:Cell Nucleus / Q000648:ultrastructure; D003593:Cytoplasm / Q000648:ultrastructure; D003713:Dendritic Cells / Q000737:chemistry / Q000473:pathology; D004549:Elastin / Q000032:analysis; D006099:Granuloma / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D008198:Lymph Nodes / Q000737:chemistry / Q000473:pathology; D008199:Lymphadenitis / Q000473:pathology*; D008264:Macrophages / Q000737:chemistry / Q000473:pathology; D013601:T-Lymphocytes / Q000737:chemistry / Q000473:pathology
D000911:Antibodies, Monoclonal; D002272:Carcinoembryonic Antigen; D004549:Elastin; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase