Direct spectroscopic determination of functional sulphydryl groups on intact cell surfaces by surface-enhanced resonance Raman scattering.
Semi-quantitative and direct determination of labelled sulphydryl groups on the surface of intact erythrocytes has been accomplished for the first time with surface-enhanced resonance Raman scattering (SERRS). The method, which involves the use of citrate-reduced silver colloids, is sensitive and selective. A 10(-8) M effective concentration of picomole quantities of sulphydryl groups was determined in the presence of the normally overwhelming signal from haemoglobin. This seminal study suggests that SERRS may be applied to other in situ, site-directed labelling experiments.
Rospendowski
B N
BN
Department of Pure and Applied Chemistry, University of Strathclyde, Glasgow, Scotland, UK.
Campbell
J M
JM
Reglinski
J
J
Smith
W E
WE
D002462:Cell Membrane / Q000737:chemistry*; D004228:Dithionitrobenzoic Acid; D004910:Erythrocyte Membrane / Q000737:chemistry*; D006801:Humans; D008832:Microchemistry; D013059:Spectrum Analysis, Raman / Q000379:methods; D013438:Sulfhydryl Compounds / Q000032:analysis* / Q000097:blood
D013438:Sulfhydryl Compounds; D004228:Dithionitrobenzoic Acid