A region of the broad-host-range plasmid RK2 causes stable in planta inheritance of plasmids in Rhizobium meliloti cells isolated from alfalfa root nodules.
We demonstrate for the first time that the broad-host-range stabilization loci from plasmid RK2 cause total retention of plasmids in cells of Rhizobium meliloti during symbiosis with alfalfa. Two derivatives of plasmid RK2, pRK290 and a 7.3-kb mini-RK2 plasmid, were stabilized in R. meliloti cells isolated from root nodules by the insertion of a 3.2-kb DNA fragment or a smaller 0.8-kb DNA fragment derived from the RK2 stabilization region.
Weinstein
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M
Department of Biology, University of California, San Diego, La Jolla 92093-0634.
Roberts
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RC
Helinski
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DR
D000332:Aerobiosis; D001426:Bacterial Proteins / Q000235:genetics; D005798:Genes, Bacterial; D005838:Genotype; D000455:Medicago sativa / Q000382:microbiology*; D010957:Plasmids; D011401:Promoter Regions, Genetic; D015183:Restriction Mapping; D016962:Sinorhizobium meliloti / Q000235:genetics* / Q000254:growth & development; D013559:Symbiosis; D014157:Transcription Factors / Q000235:genetics
D001426:Bacterial Proteins; C061338:NifA protein, Bacteria; D014157:Transcription Factors