Early changes of affinity and binding sites of progesterone and oestrogen receptors in decidua exposed to RU 486.
Sixty patients with 6-7 weeks of amenorrhoea were randomly allocated to three groups. The women in the first group (control) took a placebo 24 h before undergoing a vacuum aspiration. The patients in the second and third groups were given 200 mg of RU 486 orally, 12 or 24 h before surgical interruption of their pregnancy. Decidua were collected and frozen in liquid nitrogen. By Scatchard plot analysis, the number of cytosolic binding sites (1798 +/- 803 fmol/mg DNA) of progesterone in decidua in the control group was significantly reduced (P less than 0.01) to 696 +/- 408 or 626 +/- 179 fmol/mg DNA by RU 486 treatment for 12 or 24 h respectively. The dissociation constants of both cytosolic and nuclear progesterone receptors in RU 486-exposed decidua were increased (P less than 0.01). The number of nuclear binding sites of oestrogen receptor was significantly higher (P less than 0.05) in decidua with RU 486 treatment for 12 h (178 +/- 77 fmol/mg DNA) compared to the control (89 +/- 32 fmol/mg DNA). The results suggest that RU 486 might regulate progesterone and oestrogen receptors in the decidua of early human pregnancy, either directly or indirectly.
Shi
W L
WL
Department of Cell Biology, National Research Institute for Family Planning, Beijing, People's Republic of China.
Wang
J D
JD
Xu
L K
LK
Fu
Y
Y
Zhu
P D
PD
Qiao
G M
GM
Wang
Y Q
YQ
D001665:Binding Sites / Q000187:drug effects; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism; D003600:Cytosol / Q000187:drug effects / Q000378:metabolism; D003656:Decidua / Q000187:drug effects*; D005260:Female; D006801:Humans; D015735:Mifepristone / Q000494:pharmacology*; D011247:Pregnancy; D011960:Receptors, Estrogen / Q000187:drug effects*; D011980:Receptors, Progesterone / Q000187:drug effects*; D013997:Time Factors
D011960:Receptors, Estrogen; D011980:Receptors, Progesterone; D015735:Mifepristone