title: Use of an anti-idiotypic monoclonal antibody in studying amyloidogenic light chains in cells, urine and fibrils: pathophysiology and clinical implications.
abstract: A monoclonal anti-idiotype antibody (IgG1k MoAb 3B11D4) raised against the amyloidogenic DEP lambda chain dimer binds a conformational idiotope also present on the monoclonal DEP IgA immunoglobulin. MoAb 3B11D4 does not recognize the reduced and alkylated lambda chain monomers, nor the 15-17-kDa fibrillar light chain fragments which have the same N-terminal sequence of the urinary light chains. The lack of about 70 amino acid residues of the C terminal of the protein prevents the formation of the self-limiting dimer and may facilitate the deposition of the fragments into amyloid fibrils. MoAb 3B11D4 recognizes the plasma cell clone in bone marrow and 9% of circulating B lymphocytes. Panning experiments demonstrate that this antibody has the capability to selectively eliminate the idiotype positive cells from peripheral blood. Antibodies with these characteristics could become a useful tool for better understanding the pathogenesis of the disease and for new therapeutic options.
authors.affiliation: Clinica Medica II, University Hospital Policlinico S. Matteo, Pavia, Italy.
authors.forename: V
authors.initials: V
authors.lastname: Bellotti
authors.forename: M
authors.initials: M
authors.lastname: Stoppini
authors.forename: V
authors.initials: V
authors.lastname: Perfetti
authors.forename: I
authors.initials: I
authors.lastname: Zorzoli
authors.forename: G
authors.initials: G
authors.lastname: Marinone
authors.forename: R
authors.initials: R
authors.lastname: Invernizzi
authors.forename: L M
authors.initials: LM
authors.lastname: Zambelli
authors.forename: E
authors.initials: E
authors.lastname: Arbustini
authors.forename: M
authors.initials: M
authors.lastname: Grasso
authors.forename: G
authors.initials: G
authors.lastname: Ferri
mesh_terms: D000595:Amino Acid Sequence; D000686:Amyloidosis / Q000209:etiology* / Q000276:immunology / Q000628:therapy; D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000627:therapeutic use; D006801:Humans; D007147:Immunoglobulin Light Chains / Q000032:analysis*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008875:Middle Aged; D008969:Molecular Sequence Data
chemical_list: D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D007147:Immunoglobulin Light Chains