Induction and repair of UVB-induced cyclobutane pyrimidine dimers and (6-4) photoproducts in organ-cultured normal human skin.
To examine the induction and repair of UV-induced DNA damage, indirect immunofluorescence was performed on UVB-irradiated organ-cultured normal human skin using monoclonal antibodies specific for either cyclobutane pyrimidine dimers or (6-4) photoproducts. Nuclear immunofluorescence of cyclobutane pyrimidine dimers and (6-4) photoproducts were observed in a dose-dependent manner after UVB irradiation. The intensity of nuclear immunofluorescence of the upper epidermal layers was stronger and clearer than that of the lower epidermal layers. DNA repair time-course studies showed that both types of DNA damage could be repaired within 24 h after UVB irradiation.
Muramatsu
T
T
Department of Dermatology, Nara Medical University, Japan.
Kobayashi
N
N
Tada
H
H
Yamaji
M
M
Shirai
T
T
Mori
T
T
Ohnishi
T
T
D003503:Cyclobutanes / Q000378:metabolism*; D004249:DNA Damage; D004260:DNA Repair; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D008297:Male; D008875:Middle Aged; D009924:Organ Culture Techniques; D010777:Photochemistry; D011740:Pyrimidine Dimers / Q000378:metabolism*; D012867:Skin / Q000378:metabolism / Q000528:radiation effects*; D014466:Ultraviolet Rays / Q000009:adverse effects*
D003503:Cyclobutanes; D011740:Pyrimidine Dimers