title: Modulation of fibrinogen content in cryoprecipitate by temperature manipulation during plasma processing.
abstract: In routine blood bank production of single-donation cryoprecipitate, the introduction of a 16-hour hold at 4 degrees C, with the frozen plasma units packed into polystyrene containers, resulted in plasma prethaw temperatures of -4 degrees C to -8 degrees C. This in turn resulted in cryoprecipitate fibrinogen levels that were 214 percent of those obtained when units were thawed immediately after removal from -30 degrees C storage. In scale-model production of factor VIII concentrate, plasma warmed from -30 to -10 to -15 degrees C over 18 hours before pooling and thawing yielded cryoprecipitate fibrinogen levels that were 66 percent of those found in plasma warmed to -2 to -5 degrees C over the same period. Processing -30 degrees C plasma without a warming period led to cryoprecipitate fibrinogen levels that were 40 percent of those obtained from plasma warmed to -2 to -5 degrees C. These differences were accentuated after purification of the cryoprecipitates to an intermediate-purity factor VIII concentrate. These results suggest that simple modifications in production methods allow the fibrinogen content of cryoprecipitate to be tailored to specific uses.
authors.affiliation: Blood Product Development Section, Red Cross Blood Bank, South Melbourne, Victoria, Australia.
authors.forename: A
authors.initials: A
authors.lastname: Farrugia
authors.forename: S
authors.initials: S
authors.lastname: Grasso
authors.forename: S
authors.initials: S
authors.lastname: Douglas
authors.forename: R
authors.initials: R
authors.lastname: Harrap
authors.forename: A
authors.initials: A
authors.lastname: Oates
authors.forename: I
authors.initials: I
authors.lastname: Young
authors.forename: J
authors.initials: J
authors.lastname: James
authors.forename: G
authors.initials: G
authors.lastname: Whyte
mesh_terms: D001771:Blood Banks; D011232:Chemical Precipitation; D005169:Factor VIII / Q000096:biosynthesis; D005340:Fibrinogen / Q000032:analysis*; D005615:Freezing; D006801:Humans; D010949:Plasma / Q000737:chemistry*; D013696:Temperature; D014019:Tissue Donors
chemical_list: D005169:Factor VIII; D005340:Fibrinogen