The ANF-C receptor is not linked to adenylyl cyclase inhibition in bovine pulmonary artery endothelial cells.
The possible inhibition of adenylyl cyclase activity by atrial peptides selective for the ANF-C receptor was investigated in bovine pulmonary artery endothelial cells. In these cells isoprenaline, guanine nucleotide and forskolin dose-dependently increased activity over basal levels. In the presence of rANF(99-126), these dose-dependent increases were not reduced, nor were they affected by the ANF-C receptor selective analogue C-ANF(102-121). Furthermore, the selective analogues rANF(103-123) and des[Cys105,Cys121]rANF104-126 had no effect on basal or stimulated adenylyl cyclase activity. It can be concluded that ANF-C receptors are not linked to inhibition of adenylyl cyclase in these cells.
Kent
A
A
Department of Pharmacology, University College, Belfield, Dublin, Ireland.
Redmond
E M
EM
Keenan
A K
AK
D000067956:Adenylyl Cyclase Inhibitors; D000262:Adenylyl Cyclases / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D002460:Cell Line; D005576:Colforsin / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004730:Endothelium, Vascular / Q000201:enzymology*; D006165:Guanylyl Imidodiphosphate / Q000494:pharmacology; D007545:Isoproterenol / Q000494:pharmacology; D011651:Pulmonary Artery / Q000201:enzymology*; D017461:Receptors, Atrial Natriuretic Factor / Q000502:physiology*
D000067956:Adenylyl Cyclase Inhibitors; D005576:Colforsin; D006165:Guanylyl Imidodiphosphate; D000262:Adenylyl Cyclases; D017461:Receptors, Atrial Natriuretic Factor; D007545:Isoproterenol