Biotin-hydrazide derivatives for the development of steroid enzyme-linked immunoassays.
We are describing the synthesis and use of biotinamidocaproyl-derivatives of 18-oxocortisol-3-carboxymethoxylamine for the development of enzyme labels using the avidin-peroxidase system for the design of enzyme-linked immunoassays (ELISA). An ELISA for 18-oxocortisol and -hydroxycorticosterone was devised which showed improved sensitivity and specificity in comparison to an RIA using a tritiated tracer. The system is easy to prepare and offers the possibility to design immunoassays when no tritiated tracer is available.
Gómez-Sánchez
C E
CE
Department of Internal Medicine, University of South Florida for the Health Sciences, Tampa.
León
L M
LM
Gómez-Sánchez
E P
EP
D015069:18-Hydroxycorticosterone / Q000031:analogs & derivatives / Q000032:analysis* / Q000276:immunology; D001667:Binding, Competitive; D001710:Biotin / Q000031:analogs & derivatives*; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006834:Hydrazines; D006854:Hydrocortisone / Q000031:analogs & derivatives* / Q000032:analysis / Q000276:immunology
D006834:Hydrazines; C038135:18-oxocortisol; C029424:hydrazine; D015069:18-Hydroxycorticosterone; D001710:Biotin; D006854:Hydrocortisone