title: Application of two-color immunofluorescence staining to demonstration of T-cells and HLA-DR-bearing cells in rheumatoid synovitis.
abstract: We studied the localization of T-cells and HLA-DR antigen-bearing (DR+) cells in rheumatoid synovitis by employing an improved two-color immunofluorescent staining (TCIF) technique. With this technique we have successfully identified DR+ activated T-cells in the inflammatory synovium. T-cells expressed HLA-DR antigen when they were in contact with DR+ antigen-presenting cells (APC). In addition, activated T-cells showed characteristic distribution within the synovium: they were found around high endothelial venules, within lymphoid follicles, and in hyperplastic synovial lining, suggesting their involvement in the development of rheumatoid synovial lesions via interaction with synovial DR+ APC lineage cells. These findings may contribute to better understanding of the role of activated T-cells in the histogenesis of rheumatoid synovitis, a typical chronic inflammatory lesion.
authors.affiliation: Laboratory of Biology, Nippon Kayaku Co., Ltd., Tokyo, Japan.
authors.forename: J
authors.initials: J
authors.lastname: Itoh
authors.forename: K
authors.initials: K
authors.lastname: Kinjoh
authors.forename: A
authors.initials: A
authors.lastname: Ohyama
authors.forename: M
authors.initials: M
authors.lastname: Nose
authors.forename: M
authors.initials: M
authors.lastname: Kyogoku
mesh_terms: D001172:Arthritis, Rheumatoid / Q000378:metabolism* / Q000473:pathology; D005455:Fluorescent Antibody Technique; D006684:HLA-DR Antigens / Q000378:metabolism*; D006801:Humans; D006965:Hyperplasia; D016253:Microscopy, Immunoelectron; D013583:Synovial Membrane / Q000166:cytology; D013585:Synovitis / Q000378:metabolism* / Q000473:pathology; D013601:T-Lymphocytes / Q000378:metabolism*
chemical_list: D006684:HLA-DR Antigens