title: Incidence and phenotype restriction of lymphoid BLT-serine protease granules in spontaneously diabetes prone BB rats compared with a normal rat strain.
abstract: In diabetes prone BB rats a relative increase of serine protease (SP)-positive lymphocytes (39.8 +/- 10%) was observed in peripheral blood at the time of diabetes manifestation (DM) compared with non-diabetic healthy Sprague Dawley control rats (Co: 10.3 +/- 4%), with BB rats at age of premanifestation (PM: 14.7 +/- 4%) and beyond age of expected manifestation (non-diabetic animals, ND: 25.2 +/- 4%). Similar absolute numbers were found in diabetic BB rats in comparison with Sprague Dawley rats. In PM, absolute numbers were lower in comparison with diabetic BB rats. SP granular positivity was found restricted to OX8+ lymphocytes. SP granule-bearing OX8+ lymphocytes were more frequently seen in the BB rat strain (PM: 74.3 +/- 8%; DM: 79.4 +/- 8%; ND: 78 +/- 10%) compared with normal rats (Co: 32.5 +/- 8%). Absolute numbers were lower in PM animals in comparison with DM rats. OX8+ cells were found in a higher relative number in DM animals (49.1 +/- 7%) compared with controls (28.2 +/- 3%), PM (26.3 +/- 5%) and ND (34 +/- 2%) animals. T lymphocytes expressing the W3/25+ marker, invariably negative for SP granules, were present in a higher relative number in ND (49.8 +/- 7%) and the control group (52.3 +/- 10%) compared with PM (31 +/- 8%) and DM (38 +/- 11%) animals. Absolute numbers of the OX39+ lymphocyte subpopulation were decreased in PM and DM-BB rats in comparison with the control group.
authors.affiliation: 1st Department of Medicine, University of Vienna, Austria.
authors.forename: L
authors.initials: L
authors.lastname: Wagner
authors.forename: W
authors.initials: W
authors.lastname: Base
authors.forename: M
authors.initials: M
authors.lastname: Wiesholzer
authors.forename: V
authors.initials: V
authors.lastname: Sexl
authors.forename: C
authors.initials: C
authors.lastname: Fürnsinn
authors.forename: G
authors.initials: G
authors.lastname: Lang
authors.forename: J
authors.initials: J
authors.lastname: Froschauer
authors.forename: W
authors.initials: W
authors.lastname: Waldhäusl
mesh_terms: D000818:Animals; D003594:Cytoplasmic Granules / Q000201:enzymology; D003922:Diabetes Mellitus, Type 1 / Q000276:immunology*; D053804:Granzymes; D016131:Lymphocyte Subsets / Q000201:enzymology*; D008297:Male; D010641:Phenotype; D051381:Rats; D017207:Rats, Sprague-Dawley; D012697:Serine Endopeptidases / Q000032:analysis*
chemical_list: D053804:Granzymes; D012697:Serine Endopeptidases