Induction of c-fos gene expression by exposure to a static magnetic field in HeLaS3 cells.
Effects of a static magnetic field on cell growth and c-fos oncogene expression were investigated. HeLaS3 cells exposed to the magnetic field of 0.18-0.2 T for 1-6 days were not affected in the cell growth. Exposure to the magnetic field did not enhance the effects of X-rays or heat treatment which caused the transient cell growth delay. c-fos mRNA in the HeLaS3 cells was undetectable in untreated cells, but the expression was induced in cells by the magnetic field exposure for 2-24 h. The amounts of the mRNA expression changed time dependently with a peak at a 6-h exposure. c-fos was expressed following the heat treatment at 45 degrees C for 10 and 15 min, and the expression was further enhanced by the treatment of cells with heating followed by 4 h of the magnetic field exposure. Exposure of the cells to a static magnetic field may affect some cellular metabolic events leading to the c-fos gene expression.
Hiraoka
M
M
Department of Radiology, Faculty of Medicine, Kyoto University, Japan.
Miyakoshi
J
J
Li
Y P
YP
Shung
B
B
Takebe
H
H
Abe
M
M
D002455:Cell Division / Q000528:radiation effects; D005786:Gene Expression Regulation / Q000528:radiation effects; D016762:Genes, fos; D006367:HeLa Cells; D006358:Hot Temperature; D006801:Humans; D008280:Magnetics; D012333:RNA, Messenger / Q000032:analysis; D013997:Time Factors
D012333:RNA, Messenger