Complementation of Saccharomyces cerevisiae acid phosphatase mutation by a genomic sequence from the yeast Yarrowia lipolytica identifies a new phosphatase.
A Yarrowia lipolytica gene library was constructed in vector YRp7 and transformed into a Saccharomyces cerevisiae strain lacking both major acid phosphatase activities. A 2.18 kb genomic sequence restoring the ability to hydrolyze alpha-naphthyl phosphate was isolated. Its sequencing revealed an ORF encoding 358 amino acids without significant homology with any known phosphatase. A putative signal peptide and several possible sites for N-glycosylation were identified. Phosphate-regulated expression of the cloned gene was observed in Y. lipolytica. Disruption data favoured the hypothesis that it might encode a minor phosphatase species.
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INRA, Centre de Biotechnologies Agro-Industrielles, INA-PG, Thiverval-Grignon, France.
Le Dall
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Gaillardin
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D000135:Acid Phosphatase / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004271:DNA, Fungal; D015723:Gene Library; D005800:Genes, Fungal; D005816:Genetic Complementation Test; D006031:Glycosylation; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010957:Plasmids; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics; D004718:Saccharomycetales / Q000201:enzymology* / Q000235:genetics; D014170:Transformation, Genetic
D004271:DNA, Fungal; D000135:Acid Phosphatase