Effect of heat shock on expression of proteins not involved in the heat-shock regulon.
The effect of heat shock on the expression of some genes of Escherichia coli was tested. To avoid side effects, promoters of the genes were fused to lacZ and their expression measured by the level of beta-galactosidase. The results show that expression of umuC, recA and polB, after induction of the SOS response, was somewhat higher in the heat-shocked than in the non-shocked cells, whereas expression of ada, alkB and alkA genes, after induction of the adaptive response, was about the same. Unexpectedly, it was found that expression of lacZ from its own promoter was drastically lowered in the heat-shocked cells. This effect, however, seems not to be dependent on the induction of heat-shock proteins.
Fabisiewicz
A
A
Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw.
Janion
C
C
D004790:Enzyme Induction; D004926:Escherichia coli / Q000235:genetics*; D015870:Gene Expression; D005798:Genes, Bacterial; D005809:Genes, Regulator; D005838:Genotype; D006358:Hot Temperature; D007700:Kinetics; D007763:Lac Operon; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D013014:SOS Response, Genetics; D001616:beta-Galactosidase / Q000096:biosynthesis* / Q000235:genetics
D011993:Recombinant Fusion Proteins; D001616:beta-Galactosidase