Crystallization and preliminary X-ray investigation of bovine liver mitochondrial aldehyde dehydrogenase.
Aldehyde dehydrogenase from bovine liver mitochondria has been crystallized using the sitting drop method of vapor diffusion at 22 degrees C. The crystals formed from solutions containing, 40 mM-sodium citrate, 1 mM-NAD+ and 21% to 24% polyethylene glycol 3400 (pH 5.3 to 5.5). X-ray diffraction data collected from these crystals indicate that the crystals belong to the orthorhombic space group P2(1)2(1)2(1) with cell dimensions of a = 153.7 A, b = 159.37 A and c = 101.45 A. The crystals diffract to at least 2.9 A and a tetramer may comprise the asymmetric unit.
Hurley
T D
TD
Department of Biochemistry, Indiana University, School of Medicine, Indianapolis 46202.
Weiner
H
H
D000444:Aldehyde Dehydrogenase / Q000737:chemistry*; D000818:Animals; D002417:Cattle; D003460:Crystallization; D008930:Mitochondria, Liver / Q000201:enzymology*; D014961:X-Ray Diffraction
D000444:Aldehyde Dehydrogenase