title: A quantitative chemical study of the comb and wattle galactosaminoglycans from single comb White Leghorn roosters.
abstract: Galactosaminoglycans from mature rooster comb and wattle tissues were separated into five fractions by ethanol precipitation. An average of 90% total uronic acid was recovered in Fractions I to III. Fractions I and II were dermatan sulfate with relatively high proportions of L-iduronic acid (61 to 80%), but this uronic acid was a minor component (30%) in Fraction III, in which D-glucuronic acid was the major uronic acid. Digestion with testicular hyaluronidase suggested that most if not all of the galactosaminoglycans in Fractions I to III were copolymers containing both L-iduronic acid and D-glucuronic acid. Fractions IV and V contained much lower proportions of L-iduronic acid and showed broader electrophoresis bands than did Fraction III.
authors.affiliation: Department of Animal Science, University of Alberta, Edmonton, Canada.
authors.forename: T
authors.initials: T
authors.lastname: Nakano
authors.forename: J S
authors.initials: JS
authors.lastname: Sim
mesh_terms: D000818:Animals; D002240:Carbohydrate Sequence; D005591:Chemical Fractionation; D002645:Chickens; D003129:Comb and Wattles / Q000737:chemistry*; D008297:Male; D008969:Molecular Sequence Data; D011134:Polysaccharides / Q000032:analysis*
chemical_list: D011134:Polysaccharides; C007815:galactosaminoglycan