title: Nucleotide and deduced amino acid sequence of the canine adenovirus type 1 proteinase.
abstract: The DNA sequence of an open reading frame (ORF) corresponding to the canine adenovirus type 1 (Can1) proteinase gene was determined. A total of 1171 base pairs were sequenced from the downstream end of the HindIII-A Can1 genomic fragment, including adjacent regions corresponding to the carboxy-terminal portions of the hexon and the DNA-binding proteins. The predicted Can1 proteinase consists of 206 residues (23,325 D) of which 68% are identical and 83% are similar to the sequence of the human Ad2 proteinase. Alignment with the Ad2 proteinase identified a number of conserved residues that could form part of the catalytic triad of the enzyme.
authors.affiliation: Département de Microbiologie, Faculté de Médecine, Université de Sherbrooke, Québec, Canada.
authors.forename: F
authors.initials: F
authors.lastname: Cai
authors.forename: J M
authors.initials: JM
authors.lastname: Weber
mesh_terms: D000256:Adenoviridae / Q000201:enzymology* / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003546:Cysteine Endopeptidases / Q000737:chemistry / Q000235:genetics*; D004279:DNA, Viral / Q000235:genetics*; D004285:Dogs; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid
chemical_list: D004279:DNA, Viral; D003546:Cysteine Endopeptidases; C091827:adenain