Experimental colonic inflammation and ulceration. Permeation of a water-soluble contrast medium as a measure of 'disease' activity.
The aim of this study was to investigate the permeation of an isosmolar water-soluble X-ray contrast medium (CM) from the unperforated colon after experimental induction of inflammation and ulceration. One hundred and sixty-five male rats were included. In 110 rats 0.25 ml of 40% ethanol + 15 mg or 30 mg trinitrobenzene (TNB) was instilled into the colon, 7-9 cm proximal to the anus, inducing different degrees of inflammation. Fifty-five rats served as controls and had 0.25 ml saline instilled with the same procedure. At 7, 14, 21, and 28 days 3 ml of the non-ionic CM iodixanol was applied as an enema, and subsequently all urine was collected for the next 4 h. High-performance liquid chromatography analyses of the urine showed a positive relation between the severity of inflammation/ulceration in the colonic wall as assessed by a macroscopic damage score and the amount of CM excreted in the urine. Water-soluble contrast media may have prospects of combining functional tests of intestinal membrane dysfunction with segmental exposure control.
Andersen
R
R
Institute for Surgical Research, Rikshospitalet, Oslo, Norway.
Laerum
F
F
Bay
D
D
Aas
K
K
Halstensen
T S
TS
Stordahl
A
A
D000818:Animals; D003092:Colitis / Q000000981:diagnostic imaging / Q000378:metabolism / Q000473:pathology*; D003106:Colon / Q000000981:diagnostic imaging / Q000473:pathology; D003287:Contrast Media / Q000493:pharmacokinetics; D007413:Intestinal Mucosa / Q000378:metabolism*; D008297:Male; D010539:Permeability; D011859:Radiography; D051381:Rats; D012995:Solubility; D014283:Triiodobenzoic Acids / Q000493:pharmacokinetics*; D014456:Ulcer / Q000473:pathology
D003287:Contrast Media; D014283:Triiodobenzoic Acids; C044834:iodixanol