title: The wheat mitochondrial genome contains an ORF showing sequence homology to the gene encoding the subunit 6 of the NADH-ubiquinone oxidoreductase.
abstract: A region of the mitochondrial (mt) DNA of wheat was studied because of its homology with other plant mtDNAs. Sequence analysis revealed an open reading frame encoding a polypeptide of 247 amino acids. Comparison of the sequence of the putative polypeptide with the protein sequence data of the Swiss-Prot library reveals homology with subunit 6 of the NADH-ubiquinone complex of mitochondria from Marchantia polymorpha, Podospora anserina, Chlamydomonas reinhardtii and of chloroplasts from M. polymorpha and Oryza sativa. No similarity was detected when compared with the subunit 6 of animal mitochondria, probably due to the rapid evolution of the sequence. A single 1.2 kb transcript appears in northern RNA blots. We found 15 edited sites of which only 13 give amino acid changes. This is the first report of a mt nad6 gene in higher plants.
authors.affiliation: Laboratoire de Biologie Moléculaire Végétale associé au CNRS (UA 1128), Université Paris Sud, Orsay, France.
authors.forename: N
authors.initials: N
authors.lastname: Haouazine
authors.forename: A
authors.initials: A
authors.lastname: Pereira de Souza
authors.forename: M F
authors.initials: MF
authors.lastname: Jubier
authors.forename: D
authors.initials: D
authors.lastname: Lancelin
authors.forename: E
authors.initials: E
authors.lastname: Delcher
authors.forename: B
authors.initials: B
authors.lastname: Lejeune
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D017343:Genes, Plant / Q000235:genetics*; D016678:Genome; D008928:Mitochondria / Q000502:physiology*; D008969:Molecular Sequence Data; D016660:NAD(P)H Dehydrogenase (Quinone) / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic; D014908:Triticum / Q000201:enzymology / Q000235:genetics*
chemical_list: D012333:RNA, Messenger; D016660:NAD(P)H Dehydrogenase (Quinone)