A new system for bilayer lipid membrane capacitance measurements: method, apparatus and applications.
A new method and a new apparatus for capacitance measurements on bilayer lipid membranes are described. The membrane is charged and discharged with a constant current during the measurement. The charge-discharge cycle duration, which is proportional to the membrane capacitance, is measured. The measured time period is converted into a binary number by digital systems and then this number is either further converted into a constant capacity-proportional voltage or read out by the computer. The apparatus makes it possible to measure the capacitances of voltage-polarized membranes. Application of the apparatus to capacitance measurements of bilayer lipid membranes during their potential on the capacitance is presented. The capacitances of membranes stimulated by rectangular voltage pulses and of those stimulated by a linearly varying potential were reported.
Kalinowski
S
S
Institute of Chemistry, University of Warsaw, Białystok, Poland.
Figaszewski
Z
Z
D008051:Lipid Bilayers; D008564:Membrane Potentials; D010713:Phosphatidylcholines / Q000737:chemistry
D008051:Lipid Bilayers; D010713:Phosphatidylcholines