Continuous flow solid (gel) phase peptide synthesis using unsupported ultra-high load polymers.
A simple, manually operated, continuous flow apparatus is described for solid (gel) phase peptide synthesis. The approach uses an unsupported phenolic bead form core network at an initial matrix loading of 5 mmol g-1, the theoretical maximum. The synthesis is performed in a flow reactor under low pressure conditions. "Layered displacement" of reagent solutions and washing solvents is an essential feature that has been developed to facilitate efficient peptide synthesis. The usefulness of the present system in conjunction with N alpha Boc protected amino acids is illustrated by the syntheses of [Leu5]-enkephalin and dermorphin. The potential for scale up synthesis has also been investigated.
Coffey
A F
AF
School of Applied Sciences, Wolverhampton Polytechnic, UK.
Johnson
T
T
D000595:Amino Acid Sequence; D015740:Calcitonin Gene-Related Peptide / Q000138:chemical synthesis / Q000737:chemistry; D002625:Chemistry, Organic / Q000295:instrumentation*; D005069:Evaluation Studies as Topic; D005782:Gels; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009842:Oligopeptides / Q000138:chemical synthesis / Q000737:chemistry; D018847:Opioid Peptides; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000737:chemistry; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry; D011108:Polymers / Q000737:chemistry
D005782:Gels; D009842:Oligopeptides; D018847:Opioid Peptides; D010446:Peptide Fragments; D010455:Peptides; D011108:Polymers; C030058:dermorphin; D015740:Calcitonin Gene-Related Peptide