Enzyme-linked immunosorbent assay of colostral IgG transported into lymph and plasma in neonatal pigs.
Amounts of colostral proteins in lymph and plasma were estimated by an enzyme-linked immunosorbent assay (ELISA) after infusion of bovine colostrum into the duodenal lumen in nonsuckling neonatal pigs. The rate of immunoglobulin (Ig) G transport in lymph of the thoracic duct reached the maximal level (4.7 +/- 1.3 mg.15 min-1.kg body wt-1) within 3 h after the duodenal infusion. The rate of small protein (SP) transport more slowly increased than that of IgG. On the contrary, casein remained in the much lower level in lymph. IgG concentration in plasma increased gradually and reached a plateau level (2.5 +/- 0.9 g/l) 5 h after the infusion, but the levels of SP and casein were slowly and slightly increased in plasma. The IgG-to-casein and SP-to-casein ratios in lymph and plasma were 161:15:1 and 128:12:1 4 h after the infusion, respectively, and much higher than the value in the colostrum (IgG/SP/casein = 15:4:1). These results indicate 1) that IgG transported via lymph flow after absorption through the small intestine is faster than that via blood flow, 2) that the concentration of absorbed IgG is higher than that of absorbed SP and much higher than the concentration of absorbed casein both in the lymph and plasma, and 3) that total amount of colostral protein transported via blood flow is larger than that transported via lymph flow.
Kiriyama
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Department of Physiology, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism*; D001692:Biological Transport; D002364:Caseins / Q000097:blood; D002417:Cattle; D003126:Colostrum / Q000378:metabolism*; D004386:Duodenum; D004797:Enzyme-Linked Immunosorbent Assay; D007074:Immunoglobulin G / Q000097:blood / Q000378:metabolism*; D007267:Injections; D008196:Lymph / Q000378:metabolism*; D009994:Osmolar Concentration; D013552:Swine / Q000378:metabolism*; D013997:Time Factors
D002364:Caseins; D007074:Immunoglobulin G