title: [Evaluation of the western blot test in the follow up of children born to HIV seropositive mothers].
abstract: We have made a follow-up study of 65 children born to HIV seropositive mothers. These children were studied during the first quarter of life and subsequently every three months thereafter to measure the usefulness of the Western Blot technique in the detection of the gradual development of seroreversion, as well as in HIV infection. The study of 25 mother-child pairs allowed us to anticipate seroreversion up to three months, showing an earlier and quicker finding than by indirect immunofluorescence. In the HIV diagnosis during the first 15 months of life, the sequential study of serum by Western Blot is able to detect the reappearance of new bands, indicating the endogenous production of antibodies in the child. The circunstance is observed in 7 out of 10 infected children, showing the reappearance of bands corresponding to antibodies against p-31 and/or p-55 proteins between the second and fourth quarter of life.
authors.affiliation: Centro Nacional de Biología Celular y Retrovirus, Instituto de Salud Carlos III, Majadahonda, Madrid.
authors.forename: L
authors.initials: L
authors.lastname: Pérez Alvarez
authors.forename: D
authors.initials: D
authors.lastname: Gurbindo Gutiérrez
authors.forename: T
authors.initials: T
authors.lastname: Hernández Sampelayo
authors.forename: C
authors.initials: C
authors.lastname: Casado Herrero
authors.forename: G
authors.initials: G
authors.lastname: Contreras Carrasco
authors.forename: A
authors.initials: A
authors.lastname: García Saiz
authors.forename: R
authors.initials: R
authors.lastname: Najera Morrondo
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000175:diagnosis / Q000276:immunology / Q000635:transmission*; D000917:Antibody Formation; D015153:Blotting, Western; D005260:Female; D005500:Follow-Up Studies; D006679:HIV Seropositivity / Q000175:diagnosis / Q000276:immunology / Q000635:transmission*; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious
vernacular_title: Valoración del western blot en el seguimiento de niños nacidos de madres seropositivas al Virus de la Inmunodeficiencia Humana.