title: Ovine maternal and fetal glomerular atrial natriuretic factor receptors: response to dehydration.
abstract: In pregnancy, dehydration produces marked effects on maternal and fetal body water homeostasis including an increase in fetal urinary sodium concentration and excretion. To examine the role of fetal plasma atrial natriuretic factor (ANF) and glomerular ANF receptors in dehydration-induced natriuresis, we compared plasma ANF levels and glomerular ANF binding characteristics in dehydrated and control maternal and fetal sheep. Mean (+/- SEM) maternal and fetal plasma ANF levels in control animals (n = 9) at 132-136 days gestation were 37 +/- 3 pg/ml and 138 +/- 20 pg/ml, respectively. Although mean ANF receptor maximum binding capacities (Bmax) were significantly higher in maternal than in fetal glomeruli (83 +/- 11 vs 34 +/- 12 fmol/mg protein, respectively), the dissociation constants (Kd) for ANF binding were not different (2.7 +/- 0.6 and 3.7 +/- 1.7 x 10(-10) M, respectively). In an additional 9 animals studied after 63 +/- 4 h of water deprivation, maternal plasma ANF levels were significantly lower than in the control group (14 +/- 4 vs. 37 +/- 3 pg/ml), maternal glomerular ANF receptor Bmax values were significantly higher (732 +/- 203 vs. 83 +/- 11 fmol/mg protein), and Kd values were six-fold higher (17.0 +/- 7.1 vs. 2.7 +/- 0.6 x 10(-10) M), although this difference was only marginally significant (p = 0.06). In contrast to the adult, there was a small, nonsignificant decrease in plasma ANF levels and no difference in Bmax or Kd values between the dehydrated and euhydrated fetal animals.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Obstetrics and Pediatrics, University of California, Los Angeles School of Medicine, Torrance.
authors.forename: Y
authors.initials: Y
authors.lastname: Fujino
authors.forename: M G
authors.initials: MG
authors.lastname: Ross
authors.forename: M G
authors.initials: MG
authors.lastname: Ervin
authors.forename: R
authors.initials: R
authors.lastname: Castro
authors.forename: R D
authors.initials: RD
authors.lastname: Leake
authors.forename: D A
authors.initials: DA
authors.lastname: Fisher
mesh_terms: D000818:Animals; D001158:Arteries; D009320:Atrial Natriuretic Factor / Q000097:blood; D003681:Dehydration / Q000378:metabolism*; D005260:Female; D005312:Fetal Blood; D005333:Fetus / Q000378:metabolism*; D007678:Kidney Glomerulus / Q000196:embryology* / Q000378:metabolism; D011247:Pregnancy; D011248:Pregnancy Complications; D011270:Pregnancy, Animal / Q000097:blood / Q000378:metabolism*; D017461:Receptors, Atrial Natriuretic Factor / Q000378:metabolism*; D012756:Sheep
chemical_list: D009320:Atrial Natriuretic Factor; D017461:Receptors, Atrial Natriuretic Factor