Direct visualisation and quantification of cellular cytotoxicity using two colour flourescence.
A fluorescence method is described for the evaluation of cell death induced by cellular cytolytic activity. A green fluorescent membrane dye, D275, was used to label various target cell lines and propidium iodide (PI) uptake was used to assay cell death. Natural killer (NK), lymphokine activated killer (LAK) as well as targeted T cell mediated cellular cytotoxicity were quantified using the fluorescence method and compared to results obtained with the 51chromium (51Cr) release assay. A good correlation was found after an assay period of 4-8 h indicating that the fluorescence method is a reliable alternative to the 51Cr release assay.
Kroesen
B J
BJ
Department of Clinical Immunology, University Hospital Groningen, Netherlands.
Mesander
G
G
ter Haar
J G
JG
The
T H
TH
de Leij
L
L
D002232:Carbocyanines; D016923:Cell Death; D002860:Chromium Radioisotopes; D003602:Cytotoxicity, Immunologic; D015200:Fluoroimmunoassay; D006801:Humans; D007111:Immunity, Cellular; D066298:In Vitro Techniques; D015979:Killer Cells, Lymphokine-Activated / Q000276:immunology*; D007694:Killer Cells, Natural / Q000276:immunology*
D002232:Carbocyanines; D002860:Chromium Radioisotopes; C024286:3,3'-dioctadecylindocarbocyanine