Inhibition of human corneal epithelium with immunotoxin 454A12-rRA.
We examined the effects of the immunotoxin 454A12-rRa on proliferating and confluent human corneal epithelium (HCE) in vitro. Proliferating HCE was sensitive to 454A12-rRA in a dose-dependent fashion. At immunotoxin concentrations of 1,000 ng/ml for 7 days we observed an 86% reduction in cell counts. Confluent HCE was not sensitive to 454A12-rRA at equivalent concentrations of immunotoxin. These data confirm previous observations regarding selective sensitivity of proliferating ocular tissue to immunotoxin, but suggest that HCE is less sensitive to 454A12-rRA than other ocular cell types.
Fulcher
S F
SF
Duke University Eye Center, Durham, North Carolina.
Foulks
G N
GN
Wilkerson
M
M
Cobo
L M
LM
Jaffe
G J
GJ
Hatchell
D L
DL
D000818:Animals; D000911:Antibodies, Monoclonal; D002452:Cell Count; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003315:Cornea / Q000166:cytology*; D004261:DNA Replication / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004847:Epithelial Cells; D006801:Humans; D000922:Immunotoxins / Q000633:toxicity*; D051379:Mice; D008807:Mice, Inbred BALB C; D011990:Receptors, Transferrin; D011993:Recombinant Fusion Proteins / Q000633:toxicity*; D012276:Ricin
D000911:Antibodies, Monoclonal; D000922:Immunotoxins; D011990:Receptors, Transferrin; D011993:Recombinant Fusion Proteins; D012276:Ricin