[Isolation of protoplasts of Xanthomonas rubrilineans and their use in the study of localization of aminopeptidases].
The culture of Xanthomonas rubrilineans was able to synthesize a number of intracellular aminopeptidases. To study localization of the enzymes in the cells, a protoplasting procedure was developed providing the yield of 99.7 per cent. The following subcellular fractions were isolated: periplasmic, cytoplasmic and membranous. It was shown that alanine aminopeptidase was a cytoplasmic enzyme and glutamate peptidase was a membrane-bound enzyme.
Telesnina
G N
GN
Vasil'eva
N A
NA
Krest'ianova
I N
IN
Bartoshevich
Iu E
IuE
Sazykin
Iu O
IuO
D000626:Aminopeptidases / Q000096:biosynthesis* / Q000302:isolation & purification; D002462:Cell Membrane / Q000201:enzymology; D002498:Centrifugation; D002850:Chromatography, Gel; D003470:Culture Media; D003593:Cytoplasm / Q000201:enzymology; D066298:In Vitro Techniques; D011523:Protoplasts / Q000166:cytology / Q000201:enzymology* / Q000648:ultrastructure; D014974:Xanthomonas / Q000166:cytology* / Q000201:enzymology / Q000254:growth & development
D003470:Culture Media; D000626:Aminopeptidases
Poluchenie protoplastov Xanthomonas rubrilineans i ikh ispol'zovanie dlia izucheniia lokalizatsii aminopeptidaz.