title: Neuronal influence on B and H human blood-group antigen expression in rat cochlear cultures.
abstract: The presence of B and H human blood-group antigens was analyzed by immunocytochemistry in rat cochleas developing either in vivo or in vitro. Developing animals, on embryonic day (E) 18 and postnatal day (P) 3, were used for in vivo studies. For in vitro studies, cochleas were removed at E18 and placed for 3 or 8 days in organotypic culture either directly or after partial spiral ganglion removal. Results from epithelial regions from cochleas developing in vivo were similar to those observed in corresponding areas of direct organotypic cultures where the innervation from spiral ganglion neurons was present. Antibodies to human blood group antigens, anti B and anti AB, selectively labeled hair cells. The intensity of labeling was weak at E18, but increased at P3 in vivo or after 3-8 days in organotypic culture. Anti H antibodies showed weak labeling of the apical surface of hair cells and other epithelial cells at E18; this labeling also increased at P3 or after 3-8 days in culture. In contrast, the non-innervated regions from organotypic cultures, where ganglia were partially removed, exhibited an epithelial disorganization and no hair cell labeling with any of the antibodies studied. The present findings suggest that human blood-group antigen expression on developing cochlear hair cells of rats may be related to afferent nerve fiber influence.
authors.affiliation: Departmento Ciencias Morfologicas, Facultad de Medicina, Universidad Complutense, Madrid, Spain.
authors.forename: P
authors.initials: P
authors.lastname: Gil-Loyzaga
authors.forename: M
authors.initials: M
authors.lastname: Remezal
authors.forename: R
authors.initials: R
authors.lastname: Oriol
mesh_terms: D000017:ABO Blood-Group System / Q000032:analysis*; D000818:Animals; D000831:Animals, Newborn; D000906:Antibodies; D000911:Antibodies, Monoclonal; D002454:Cell Differentiation; D003051:Cochlea / Q000196:embryology* / Q000254:growth & development / Q000294:innervation; D003714:Denervation; D015870:Gene Expression; D006198:Hair Cells, Auditory / Q000276:immunology*; D007150:Immunohistochemistry; D009024:Morphogenesis; D009924:Organ Culture Techniques; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000017:ABO Blood-Group System; D000906:Antibodies; D000911:Antibodies, Monoclonal