[Development and use of a method of determination of specific immunoglobulin G based on immunoenzyme analysis in the diagnosis of pulmonary aspergillosis].
Enzyme-linked immunoassay (ELISA) was employed for determination of specific immunoglobulin G to Aspergillus fumigatus (A.f.). Culture filtrate of A.f. was used as antigen. Optimal conditions of ELISA were found. Serum of 138 asthmatic patients with total IgE level more than 500 PRU/ml were analysed for determining the limits of IgG to A.f. concentrations.
Vlasenko
S Iu
SIu
Lebedin
Iu S
IuS
D000293:Adolescent; D000328:Adult; D000368:Aged; D000908:Antibodies, Fungal / Q000032:analysis*; D001228:Aspergillosis / Q000175:diagnosis* / Q000276:immunology; D001232:Aspergillus fumigatus / Q000276:immunology*; D001249:Asthma / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis*; D008172:Lung Diseases, Fungal / Q000175:diagnosis* / Q000276:immunology; D008297:Male; D008875:Middle Aged
D000908:Antibodies, Fungal; D007074:Immunoglobulin G
Razrabotka i primenenie metoda opredeleniia spetsificheskogo immunoglobulina G na osnove immunofermentnogo analiza dlia diagnostiki aspergilleza legkikh.