Involvement of a receptor for neurite outgrowth factor (NOFR) in cerebellar neurogenesis.
A receptor for neurite outgrowth factor (82 kDa membrane protein, NOFR) was detected in the developing chick cerebellum by immuno- and ligand blots. In immunohistochemical study, NOFR was maximally expressed in the external granular layer of cerebellum at embryonic day 10-12 and gradually decreased until embryonic day 18. Neurite outgrowth and cell migration induced by NOF from cerebellar explants were completely suppressed by the addition of anti-NOFR IgG. These results suggest that NOFR plays an important role in the cerebellar neurogenesis.
Kato
S
S
Department of Pharmacology, Osaka University School of Medicine, Japan.
Taniura
H
H
Taira
E
E
Miki
N
N
D000818:Animals; D002531:Cerebellum / Q000166:cytology / Q000196:embryology* / Q000378:metabolism; D002642:Chick Embryo; D004591:Electrophoresis, Polyacrylamide Gel; D005314:Embryonic and Fetal Development; D007074:Immunoglobulin G; D007150:Immunohistochemistry; D008970:Molecular Weight; D009414:Nerve Growth Factors / Q000276:immunology / Q000378:metabolism / Q000494:pharmacology*; D016501:Neurites / Q000187:drug effects / Q000502:physiology; D009924:Organ Culture Techniques; D017978:Receptors, Growth Factor; D011971:Receptors, Immunologic / Q000302:isolation & purification / Q000378:metabolism / Q000502:physiology*
D007074:Immunoglobulin G; D009414:Nerve Growth Factors; D017978:Receptors, Growth Factor; D011971:Receptors, Immunologic; C076866:neurite outgrowth factor receptor