Detection of alpha S1-casein in vomit from bottle-fed babies by enzyme-linked immunosorbent assay.
This study describes a sensitive enzyme-linked immunosorbent assay (ELISA) using rabbit anti-bovine alpha S1-casein antibody for the detection of commercial milk and milk-containing vomit. The antibody does not react with other human body fluids such as breast milk. The stability of alpha S1-casein antigenic activity was examined after storage at different temperatures and enzyme digestion. There was no decrease after storage for one year at room temperature but 40% of the activity was lost after 6 months at 37 degrees C. Enzyme digestion (6 hours, 37 degrees C) resulted in 65 approximately 70% loss of activity but the antibody reacted with the peptide fragments of alpha S1-casein. Vomit samples from 3 normal infants were tested by ELISA, and alpha S1-casein could be detected in 1 cm2 stain.
Sato
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Criminal Investigation Laboratory, Kanagawa Prefectural Police, Yokohama, Japan.
D000818:Animals; D001903:Bottle Feeding; D002364:Caseins / Q000737:chemistry* / Q000276:immunology; D003429:Cross Reactions; D004063:Digestion; D004591:Electrophoresis, Polyacrylamide Gel; D010450:Endopeptidases / Q000494:pharmacology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000592:standards*; D005069:Evaluation Studies as Topic; D005554:Forensic Medicine / Q000379:methods / Q000592:standards*; D006358:Hot Temperature / Q000009:adverse effects; D006801:Humans; D007223:Infant; D008892:Milk / Q000737:chemistry*; D011015:Pneumonia, Aspiration / Q000209:etiology; D012680:Sensitivity and Specificity; D013398:Sudden Infant Death / Q000209:etiology; D014839:Vomiting / Q000150:complications / Q000175:diagnosis* / Q000453:epidemiology
D002364:Caseins; D010450:Endopeptidases