title: Activation of human monocyte chemiluminescence response by acylpoly(1,3)galactosides derived from Klebsiella pneumoniae.
abstract: The stimulating activity of several preparations isolated from a membrane proteoglycan of a nonencapsulated smooth strain of Klebsiella pneumoniae (Kp-MPG) on the oxidative burst of human blood monocytes was assessed by luminol-enhanced chemiluminescence (CL). Five Kp derivatives were studied: a 34-kd acylpoly(1,3)galactoside (APG), obtained by drastic alkaline hydrolysis and purified by chromatography; an APG preparation subjected to acid hydrolysis that removed the core part and all fatty acids, leaving intact the galactose chain of APG (GC-APG); an APG preparation subjected to mild oxidation (ox APG); a preparation obtained by mild alkaline hydrolysis of Kp-MPG, containing additional ester-linked C14 and C16 fatty acids bound to the APG molecule (EFA-APG); and a polymer of the latter compound, APG pol. EFA-APG directly stimulated monocyte CL, whereas Kp-MPG, APG pol, and the whole bacterial cells had little or no activity. APG itself and ox APG induced a weaker response than EFA-APG. Polymyxin B sulfate completely inhibited the CL response to bacterial lipopolysaccharide (LPS) but not to EFA-APG. The stimulating action of EFA-APG on blood monocytes was dependent on the extracellular levels of both calcium and magnesium. Preincubation of monocytes with monoclonal antibody anti-Mac-1 directed against CD11b, the alpha chain of complement receptor type 3 (CR3; CD11b/CD18), strongly inhibited CL activation by EFA-APG and to a lesser extent CL activation by unopsonized zymosan and rough LPS. Altogether, these findings provide indirect evidence for the contribution of the CD11b/CD18 integrin in the functional interaction of EFA-APG with monocyte membranes. They demonstrate the role of fatty acids in the triggering of monocyte oxidative burst, while the polysaccharide chain itself does not contribute to induction of the CL response in this model. In keeping with the effects of EFA-APG and APG, we show that the monocyte CL response was triggered by bacterial LPS from the rough strain of Salmonella minnesota Re 595 and its lipid A, but not by LPS from smooth strains, again suggesting a critical role for the lipid moiety.
authors.affiliation: Research Center Maisonneuve-Rosemont Hospital, University of Montréal, Québec, Canada.
authors.forename: E
authors.initials: E
authors.lastname: Kouassi
authors.forename: Z
authors.initials: Z
authors.lastname: Hmama
authors.forename: G
authors.initials: G
authors.lastname: Lina
authors.forename: J
authors.initials: J
authors.lastname: Vial
authors.forename: F
authors.initials: F
authors.lastname: Faure-Barba
authors.forename: G
authors.initials: G
authors.lastname: Normier
authors.forename: H
authors.initials: H
authors.lastname: Binz
authors.forename: J P
authors.initials: JP
authors.lastname: Revillard
mesh_terms: D002118:Calcium / Q000502:physiology; D002240:Carbohydrate Sequence; D005697:Galactosides / Q000276:immunology*; D006801:Humans; D066298:In Vitro Techniques; D007711:Klebsiella pneumoniae / Q000276:immunology*; D008050:Lipid A / Q000276:immunology; D008070:Lipopolysaccharides / Q000276:immunology; D008163:Luminescent Measurements; D016177:Macrophage-1 Antigen / Q000276:immunology / Q000502:physiology; D008274:Magnesium / Q000502:physiology; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009000:Monocytes / Q000502:physiology*; D011112:Polymyxin B / Q000494:pharmacology; D011135:Polysaccharides, Bacterial / Q000276:immunology*; D011509:Proteoglycans / Q000276:immunology*; D016897:Respiratory Burst
chemical_list: D005697:Galactosides; D008050:Lipid A; D008070:Lipopolysaccharides; D016177:Macrophage-1 Antigen; D011135:Polysaccharides, Bacterial; D011509:Proteoglycans; D008274:Magnesium; D011112:Polymyxin B; D002118:Calcium