title: Six strains of human immunodeficiency virus type 1 isolated in Japan and their molecular phylogeny.
abstract: Five strains of human immunodeficiency virus type 1 (HIV-1) were isolated from five Japanese hemophilia patients. Two isolates, HIV-1[GUN-1] and HIV-1[GUN-2], were from brother patients with hemophilia B and the other three isolates, HIV-1[GUN-3], HIV-1[GUN-4], and HIV-1[GUN-5], were from hemophilia A patients. Another HIV-1 strain, HIV-1[GUN-6], was isolated from a Canadian male homosexual with AIDS. The restriction endonuclease cleavage maps of the proviral genomes of these six HIV-1 strains revealed that they were apparently different from each other. The phylogenetic trees constructed using restriction maps and nucleotide sequences were quite similar, indicating that phylogenetic analyses of Japanese HIV-1 isolates can be done using restriction maps of the proviruses. Phylogenetic analyses showed that they were more closely related to HIV-1s which had been reported to be isolated from homosexual patients in the United States than those isolated from African patients. In particular, GUN-1 and GUN-2 isolates were on the branch of a San Francisco isolate, ARV2, while GUN-5 and GUN-6 isolates were on the branch of HTLV-IIIB-related isolates.
authors.affiliation: Department of Hygiene, Gunma University School of Medicine, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Shimizu
authors.forename: Y
authors.initials: Y
authors.lastname: Takeuchi
authors.forename: T
authors.initials: T
authors.lastname: Naruse
authors.forename: M
authors.initials: M
authors.lastname: Inagaki
authors.forename: E
authors.initials: E
authors.lastname: Moriyama
authors.forename: T
authors.initials: T
authors.lastname: Gojobori
authors.forename: H
authors.initials: H
authors.lastname: Hoshino
mesh_terms: D000386:AIDS-Related Complex / Q000150:complications / Q000382:microbiology; D000163:Acquired Immunodeficiency Syndrome / Q000150:complications / Q000382:microbiology; D015139:Blotting, Southern; D015658:HIV Infections / Q000150:complications / Q000382:microbiology*; D015497:HIV-1 / Q000145:classification* / Q000235:genetics; D006467:Hemophilia A / Q000150:complications / Q000382:microbiology; D002836:Hemophilia B / Q000150:complications / Q000382:microbiology; D006801:Humans; D007564:Japan; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D015183:Restriction Mapping