A refocused and optimized HNCA: increased sensitivity and resolution in large macromolecules.
A 3D optimized, refocused HNCA experiment is described. It is demonstrated to yield a dramatic increase in sensitivity when applied to [13C, 15N]-labeled human carbonic anhydrase II, a 29-kDa protein. The reasons for the gain in sensitivity are discussed, and 3 distinct areas for further development are indicated.
Farmer
B T
BT
Department of Research and Development, Varian Associates, Palo Alto, CA 94304.
Venters
R A
RA
Spicer
L D
LD
Wittekind
M G
MG
Müller
L
L
D002247:Carbon Isotopes; D002256:Carbonic Anhydrases / Q000737:chemistry*; D006801:Humans; D007527:Isoenzymes / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008433:Mathematics; D009587:Nitrogen Isotopes; D011487:Protein Conformation
D002247:Carbon Isotopes; D007527:Isoenzymes; D009587:Nitrogen Isotopes; D002256:Carbonic Anhydrases