Iron(III)-binding polypeptide in human cord and adult serum: isolation, purification and partial characterization.
A low-molecular-weight, non-transferrin-bound, Fe(III)-binding polypeptide has been isolated and purified from normal human cord and adult sera by gel-filtration and high-performance liquid chromatography. The polypeptide was traced isotopically with 59Fe(III)-chloride and high voltage paper electrophoresis. This Fe(III)-binding polypeptide has been partially characterized, and found to be biochemically distinct from other known Fe(III)-binding proteins, such as transferrin, lactoferrin and ferritin. Furthermore, the electrophoretic mobility and amino-acid composition distinguish the polypeptide from other physiological iron chelators such as a previously described Fe(III)-citrate complex. The polypeptide is highly hydrophilic, rich in lysine residues and phosphorylated. The observed positive charge of the polypeptide is suggested to originate from these lysine residues. The molecular mass of this polypeptide is estimated to be approx. 2500 Da, which is in close agreement with previous reports and is consistent with amino-acid analysis data. Cord serum levels of the polypeptide were significantly higher than adult serum levels. The degree of Fe(III) specificity and the function of the Fe(III)-polypeptide have not been ascertained at the present time. It is possible that the polypeptide may compete for Fe(III) with transferrin and is involved in the mobilization and transportation of iron by some as yet unknown mechanism.
Stojkovski
S
S
Department of Biochemistry Research, Hospital for Sick Children, Toronto, Ontario, Canada.
Goumakos
W
W
Sarkar
B
B
D000328:Adult; D002352:Carrier Proteins / Q000097:blood* / Q000302:isolation & purification; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D004590:Electrophoresis, Paper; D005312:Fetal Blood; D006801:Humans; D007501:Iron; D033862:Iron-Binding Proteins; D033863:Transferrin-Binding Proteins
D002352:Carrier Proteins; D033862:Iron-Binding Proteins; D033863:Transferrin-Binding Proteins; D007501:Iron