title: Immobilization of alpha-amylase into photographic gelatin by chemical cross-linking.
abstract: alpha-Amylase was immobilized into photographic gelatin by chemical cross-linking with chromium (III) acetate and chromium (III) sulphate. Cellulose triacetate film strips, enabled simple handling when coated with an alpha-amylase-gelatin mixture, accomplishing a high degree of durability during consecutive immersions into reaction media. The optimum conditions for pH, substrate concentration, temperature, incubation time and storing conditions of free and immobilized alpha-amylase were determined. The effect of use number on activity and the effect of resting on reuse number were also determined. Photographic gelatin was found to be a very efficient natural polymer, due to its extraordinary diffusion characteristics for immobilization as a carrier.
authors.affiliation: Department of Chemistry, Middle East Technical University, Ankara, Turkey.
authors.forename: Z
authors.initials: Z
authors.lastname: Bayramoglu
authors.forename: U
authors.initials: U
authors.lastname: Akbulut
authors.forename: S
authors.initials: S
authors.lastname: Sungur
mesh_terms: D003432:Cross-Linking Reagents; D004800:Enzymes, Immobilized / Q000378:metabolism*; D005780:Gelatin; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D010781:Photography; D012995:Solubility; D013696:Temperature; D013997:Time Factors; D000516:alpha-Amylases / Q000378:metabolism*
chemical_list: D003432:Cross-Linking Reagents; D004800:Enzymes, Immobilized; D005780:Gelatin; D000516:alpha-Amylases