Regulation of the maize HRGP gene expression by ethylene and wounding. mRNA accumulation and qualitative expression analysis of the promoter by microprojectile bombardment.
The expression of the maize gene coding for a hydroxyproline-rich glycoprotein (HRGP) has been studied by measuring the mRNA accumulation after wounding or ethylene treatment. RNA blot and in situ hybridization techniques have been used. The temporal and tissue-specific expression has been observed: the cells related to the vascular system show the more intense HRGP mRNA accumulation. Transcriptional constructions of the maize HRGP promoter have been tested on different maize tissues by microbombarding. A 582 bp promoter is able to direct the expression of the gus gene on calli and young leaves. Constructions having shorter promoter sequences lose this ability. The 582 bp construction retains the general specificity of expression observed for the HRGP gene.
Tagu
D
D
Departament de Genètica Molecular, CID-CSIC, Barcelona, Spain.
Walker
N
N
Ruiz-Avila
L
L
Burgess
S
S
Martìnez-Izquierdo
J A
JA
Leguay
J J
JJ
Netter
P
P
Puigdomènech
P
P
D005030:Ethylenes / Q000494:pharmacology*; D005786:Gene Expression Regulation; D017343:Genes, Plant; D005966:Glucuronidase / Q000096:biosynthesis / Q000235:genetics; D006023:Glycoproteins / Q000235:genetics*; D006651:Histocytochemistry; D009693:Nucleic Acid Hybridization; D010940:Plant Proteins / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D013997:Time Factors; D014018:Tissue Distribution; D014158:Transcription, Genetic / Q000187:drug effects*; D014162:Transfection; D003313:Zea mays / Q000187:drug effects / Q000235:genetics
D005030:Ethylenes; D006023:Glycoproteins; D010940:Plant Proteins; D011994:Recombinant Proteins; C005488:extensin protein, plant; C036216:ethylene; D005966:Glucuronidase