Correlation of extracellular matrix components with the cytoarchitecture of mouse Peyer's patches.
The distribution patterns of extracellular matrix elements were determined to ascertain whether they play a role in the localization of lymphocytes in discrete T-cell, B-cell and dome antigen-processing domains within Peyer's patches. Antibodies against collagen types I, III and IV, laminin and fibronectin were applied to cryosections of mouse Peyer's patches and localized by direct or indirect immunoperoxidase methods. T-cell domains were identified with a monoclonal antibody against Thy-1.2. Labeled reticular fibers in distinctive patterns were more numerous in parafollicular and dome areas than within follicles. Germinal centers contained few such fibers. In parafollicular areas, fibers were oriented predominantly toward follicle domes; their distribution corresponded to T-cell zones and lymphocyte traffic areas, with their orientation being parallel to the migration pathways of lymphocytes from high endothelial venules to the antigen-processing domes. Subepithelial and subendothelial basal laminae were immunopositive for type-IV collagen, laminin and fibronectin. The dome subepithelial basal lamina had pore-like discontinuities through which lymphocytes migrated to and from the epithelium. The correspondence of the distribution patterns of extracellular matrix to specific functional domains of Peyer's patches suggests that this matrix provides a structural framework for lymphocyte migration and localization.
Ohtsuka
A
A
Cell Biology and Aging Section, VA Medical Center, San Francisco, CA.
Piazza
A J
AJ
Ermak
T H
TH
Owen
R L
RL
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D001402:B-Lymphocytes / Q000737:chemistry / Q000166:cytology / Q000502:physiology; D002465:Cell Movement / Q000502:physiology; D003094:Collagen / Q000032:analysis* / Q000276:immunology; D005109:Extracellular Matrix / Q000737:chemistry* / Q000648:ultrastructure; D005260:Female; D005353:Fibronectins / Q000032:analysis* / Q000276:immunology; D007150:Immunohistochemistry; D007797:Laminin / Q000032:analysis* / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008854:Microscopy, Electron; D010581:Peyer's Patches / Q000737:chemistry / Q000166:cytology* / Q000502:physiology; D013601:T-Lymphocytes / Q000737:chemistry / Q000166:cytology / Q000502:physiology
D000911:Antibodies, Monoclonal; D005353:Fibronectins; D007797:Laminin; D003094:Collagen