Immunoreactivity of skate electrocytes towards monoclonal antibodies against human dystrophin and dystrophin-related (DMDL) protein.
Monoclonal antibodies against human dystrophin have been used to demonstrate the existence of a dystrophin-like protein in the electrocytes of skate electric organ. This protein is also present in skate muscle and resembles that found in Torpedo electric organ. Monoclonal antibodies against a human autosomal homologue of dystrophin (DMDL protein) did not detect a similar protein in skate or Torpedo. Immunocytochemical staining of the innervated and non-innervated faces of the electrocyte membrane was obtained using the anti-dystrophin antibodies only.
Dowdall
M J
MJ
Department of Life Science, University of Nottingham, UK.
Ellis
J M
JM
Nguyen thi Man
Morris
G E
GE
D000818:Animals; D000911:Antibodies, Monoclonal; D015153:Blotting, Western; D003598:Cytoskeletal Proteins / Q000032:analysis* / Q000276:immunology; D016189:Dystrophin / Q000032:analysis* / Q000276:immunology; D004557:Electric Organ / Q000737:chemistry / Q000166:cytology*; D006801:Humans; D007150:Immunohistochemistry; D008565:Membrane Proteins; D012860:Skates, Fish; D014101:Torpedo; D049411:Utrophin
D000911:Antibodies, Monoclonal; D003598:Cytoskeletal Proteins; D016189:Dystrophin; D008565:Membrane Proteins; D049411:Utrophin