Role of monoclonal antibody J-23 in inducing acrosome reactions in capacitated mouse spermatozoa.
A monoclonal antibody (J-23) to the 15 kDa component on the sperm head, the acceptor, which functions in zona binding, was shown to induce the acrosome reaction in capacitated cells, but not in fresh cells. The antibody recognized its epitope in the acrosomal cap region of fresh spermatozoa and in the equatorial region on washed and capacitated spermatozoa. However, equatorial expression did not depend on the acrosome reaction, since washing fresh spermatozoa increased the percentage with equatorial fluorescence, but did not increase the percentage with reacted acrosomes. The data indicate that the acrosome reaction can be induced in capacitated spermatozoa in the absence of zona glycoproteins.
Aarons
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Department of Biology, University of Alabama, Birmingham 35294-1170.
Battle
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Boettger-Tong
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Poirier
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D000177:Acrosome / Q000502:physiology*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D002478:Cells, Cultured; D005089:Exocytosis / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D007075:Immunoglobulin M / Q000276:immunology*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D013075:Sperm Capacitation / Q000502:physiology*; D013077:Sperm Head / Q000276:immunology; D013094:Spermatozoa / Q000166:cytology
D000911:Antibodies, Monoclonal; D007075:Immunoglobulin M