Determination of active 5-(N,N-hexamethylene)amiloride in pig plasma.
A simple, cheap and specific quantitative method for the determination of the selective Na+/H+ exchange inhibitor, 5-(N,N-hexamethylene)amiloride, in plasma and aqueous solutions has been developed. The method involves extraction with ethyl acetate, thin-layer chromatography and spectrofluorodensitometry. The compound was separated from several unidentified metabolites in plasma. The detection limit was 6 x 10(-7) M. The calculated metabolic extraction by the liver was 29%, and the plasma half-life was 12.8 min. The free, active concentration of 5-(N,N-hexamethylene)amiloride was 19.4% of the total concentration, as determined by equilibrium dialysis.
Holthe
M R
MR
Institute for Experimental Medical Research, Ullevaal University Hospital, Oslo, Norway.
Brørs
O
O
Cragoe
E J
EJ
Veel
T
T
Raeder
M
M
D000584:Amiloride / Q000031:analogs & derivatives* / Q000097:blood; D000818:Animals; D002855:Chromatography, Thin Layer / Q000379:methods*; D003720:Densitometry / Q000379:methods*; D013552:Swine
C057360:5-(N,N-hexamethylene)amiloride; D000584:Amiloride