Neutralizing monoclonal antibodies against alpha and beta subunits of the Ustilago maydis virus encoded toxin.
The toxins secreted by Ustilago maydis are encoded by dsRNA viruses. The KP6 toxin encoded by subtype P6 consists of two polypeptides alpha and beta, which are not covalently bound. Neutralizing monoclonal antibodies (MoAbs) were raised against each subunit. Some of the anti-beta MoAbs identify different epitopes in the antigen. The MoAbs were used to affinity purify alpha and beta polypeptides from culture media and to detect the precursor of the mature toxin.
Ginzberg
I
I
Department of Microbiology-Biotechnology, G.S. Wise Faculty of Life Sciences, Tel-Aviv University, Ramat-Aviv, Israel.
Rosenblum
S
S
Koltin
Y
Y
Smorodinsky
N I
NI
D000911:Antibodies, Monoclonal / Q000032:analysis*; D000918:Antibody Specificity; D009183:Mycotoxins / Q000276:immunology*; D009500:Neutralization Tests; D012328:RNA Viruses / Q000276:immunology* / Q000472:pathogenicity; D014588:Ustilago / Q000276:immunology* / Q000472:pathogenicity; D014764:Viral Proteins
D000911:Antibodies, Monoclonal; D009183:Mycotoxins; C051654:Ustilago maydis toxin p6, Ustilago maydis virus; D014764:Viral Proteins