title: Presence of calcitonin-like immunoreactivity (iCT) in human prostate gland: evidence for iCT secretion by cultured prostate cells.
abstract: Immunoreactive calcitonin (iCT) has been detected in human prostate tissue extracts as well as seminal plasma. The present studies were undertaken to examine whether iSCT (immunoreactive salmon CT-like human peptide) co-exists with iHCT (thyroid CT-like substance) in human prostate tissue extracts, and whether these substances are secreted by primary prostate cells in culture. Since the local secretion of these substances seems to increase in some neoplasms, a second objective of the study was to examine whether basal secretion of iCTs from primary prostate cells is increased in carcinoma. The present results have shown that both iHCT and iSCT were present in prostate tissue extracts. The mean iHCT levels in extracts of benign hyperplastic prostates (BPH) were 0.59 ng/g prostate, and these were significantly lower than iHCT concentrations in prostatic carcinoma (PC) (2.53 ng/g). No significant differences in their iSCT contents were observed. However, the results from culture of over 90 individual prostate tissue specimens from BPH or PC indicate that primary prostate cells secreted detectable quantities of iSCT and the basal release of this material from PC prostate cultures was almost four-fold higher than that from BPH prostate cultures. These results suggest that a CT-like immunoreactive material is secreted by primary prostate cells in culture, and the basal secretion of this material is significantly higher in PC cells as compared to BPH cells. Endogenous secretion of prostatic CT, and the elevation of its expression in PC suggest that it may serve as a regulatory factor in the pathophysiology of the prostate gland.
authors.affiliation: Department of Urologic Surgery, University of Kansas Medical Center, Kansas City 66103.
authors.forename: G V
authors.initials: GV
authors.lastname: Shah
authors.forename: M J
authors.initials: MJ
authors.lastname: Noble
authors.forename: M
authors.initials: M
authors.lastname: Austenfeld
authors.forename: J
authors.initials: J
authors.lastname: Weigel
authors.forename: L J
authors.initials: LJ
authors.lastname: Deftos
authors.forename: W K
authors.initials: WK
authors.lastname: Mebust
mesh_terms: D000368:Aged; D000704:Analysis of Variance; D002116:Calcitonin / Q000276:immunology / Q000378:metabolism*; D046508:Culture Techniques; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D011467:Prostate / Q000378:metabolism*; D011471:Prostatic Neoplasms / Q000378:metabolism*; D011863:Radioimmunoassay; D012661:Semen / Q000737:chemistry
chemical_list: D002116:Calcitonin