Purine modulation of prostaglandin F2 alpha (PGF2 alpha)-induced premature luteolysis in vivo in nonpregnant sheep.
In two separate experiments, control and PGF2 alpha-treated unilaterally ovariectomized ewes received vehicle or adenosine every four hours through an exteriorized catheter installed either to parafuse the ovarian vascular pedicle of the remaining ovary containing a corpus luteum (CL) or to infuse intrauterine ipsilateral to the remaining ovary with the CL. Infusions were given every four hours from day 7 through day 22 postestrus or until ewes returned to estrus. Chronic infusion of vehicle intrauterine or parafusion of the ovarian vascular pedicle with vehicle in PGF2 alpha-treated ewes shortened the interestrous interval compared to controls (P less than 0.05). However, chronic parafusion of the ovarian vascular pedicle with adenosine in PGF2 alpha-treated ewes prevented a PGF2 alpha-induced premature luteolysis (P less than 0.05) but not when adenosine was infused chronically intrauterine (P greater than 0.05). It is concluded that adenosine can block a PGF2 alpha-induced premature luteolysis in vivo and may have roles in regulation of luteal secretion of progesterone but it is probably not the embryonic antiluteolysin of early pregnancy in ewes.
Weems
C W
CW
Department of Animal Sciences, University of Hawaii at Manoa, Honolulu 96822.
Weems
Y S
YS
Lee
C N
CN
Vincent
D L
DL
D000241:Adenosine / Q000494:pharmacology*; D000818:Animals; D003338:Corpus Luteum / Q000187:drug effects*; D015237:Dinoprost / Q000037:antagonists & inhibitors*; D005260:Female; D007263:Infusions, Parenteral; D011374:Progesterone / Q000378:metabolism; D012756:Sheep; D013997:Time Factors
D011374:Progesterone; D015237:Dinoprost; D000241:Adenosine