A complement-dependent enzyme immunoassay (C-EIA) with increased sensitivity for IgM-rich rabbit sera.
An enzyme immunoassay involving activation of complement (C-EIA) was developed for rabbit polyclonal IgM antibodies against lipid A and lipopolysaccharide antigens. C-EIA was significantly higher in sensitivity for IgM-rich rabbit sera compared to EIA using anti-immunoglobulin secondary antibodies. Hence, C-EIA should be useful for the detection of weak IgM reactivities in rabbit sera, especially after short-time immunizations. Selective inhibition of both complement pathways indicated that C-EIA measures activation of the classical pathway.
Kuhn
H M
HM
Division of Biochemical Microbiology, Forschungsinstitut Borstel, Germany.
Brade
L
L
Brade
H
H
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D003167:Complement Activation; D007124:Immunoenzyme Techniques; D007075:Immunoglobulin M / Q000032:analysis*; D008070:Lipopolysaccharides / Q000276:immunology; D011817:Rabbits
D000911:Antibodies, Monoclonal; D007075:Immunoglobulin M; D008070:Lipopolysaccharides