Use of a recombinant Dictyocaulus viviparus antigen in an enzyme-linked immunosorbent assay for immunodiagnosis of bovine dictyocaulosis.
A specific recombinant antigen was evaluated for its immunodiagnostic potential in an enzyme-linked immunosorbent assay (ELISA). The antigen was used as a glutathione S-transferase (GST) fusion protein (DvGST3-14) or as a pure recombinant parasite protein (Dv3-14). A total of 55 sera from cattle experimentally infected with Dictyocaulus viviparus, 24 sera from naturally infected cattle and 25 sera from helminth-free cattle were examined. ELISA results obtained for the sera from experimental infections showed a calculated specificity of greater than 99% for both antigen preparations and a sensitivity of 93% (DvGST3-14) and 91% (Dv3-14). For field sera from natural infections, the specificity was calculated to be 90% (DvGST3-14) and greater than 99% (Dv3-14) and the sensitivity, 85% (DvGST3-14) and 70% (Dv3-14).
Schnieder
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Institute of Parasitology, School of Veterinary Medicine Hannover, Federal Republic of Germany.
D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D000947:Antigens, Helminth; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis*; D004021:Dictyocaulus / Q000276:immunology*; D004022:Dictyocaulus Infections / Q000175:diagnosis*; D004797:Enzyme-Linked Immunosorbent Assay; D011237:Predictive Value of Tests; D011994:Recombinant Proteins; D012044:Regression Analysis; D012680:Sensitivity and Specificity
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D011994:Recombinant Proteins