Cyclophosphamide-induced suppressor cells in nude mice.
Lymphocytes regenerated after treatment with a high dose of cyclophosphamide (CY) were characterized in nude mice. Ten days after a single injection of 200 mg/kg CY into nude mice, regenerated spleen cells suppressed in vitro primary and second antibody production against sheep red blood cells. The CY-treated spleen cells exhibited normal natural killer (NK) activity, very low B and T cell content, but increases in cell surface charge [electrophoretic mobility (EPM)] and histamine receptors. The suppressor cells could not be removed by treatment with anti-Thy-1 plus complement (C), or treatment with antiasialo GM1 (aGM1) plus C, which abrogated NK activity. It was concluded that CY-treated spleen cells, which exhibited high EPM and histamine receptors, comprise the natural suppressor cells which are Ig-, Thy-1- and aGM-1.
Shimizu
M
M
Department of Cancer Therapeutics, Tokyo Metropolitan Institute of Medical Science, Japan.
Sabolovic
D
D
Ozawa
H
H
Iwaguchi
T
T
D000818:Animals; D000917:Antibody Formation / Q000187:drug effects; D000954:Antigens, Surface / Q000097:blood*; D002469:Cell Separation; D003165:Complement System Proteins / Q000494:pharmacology; D003520:Cyclophosphamide / Q000494:pharmacology*; D004586:Electrophoresis; D004912:Erythrocytes / Q000276:immunology*; D005434:Flow Cytometry; D016130:Immunophenotyping; D007694:Killer Cells, Natural / Q000187:drug effects; D051379:Mice; D008819:Mice, Nude; D011968:Receptors, Histamine / Q000187:drug effects*; D012756:Sheep; D050378:T-Lymphocytes, Regulatory / Q000187:drug effects* / Q000276:immunology
D000954:Antigens, Surface; D011968:Receptors, Histamine; D003520:Cyclophosphamide; D003165:Complement System Proteins