Neurochemical changes in rats chronically treated with a high concentration of manganese chloride.
Several neurochemical parameters were studied in brain regions of rats chronically treated with a high concentration of manganese chloride (20 mg MnCl2.4H2O per ml. of drinking water) throughout development until adulthood. Large increases in Mn accumulation were found in all brain regions (hypothalamus, +530%; striatum, +479%; other regions, +152 to +250%) of Mn-treated adult rats. In these animals, Ca levels were decreased (-20 to -46%) in cerebellum, hypothalamus, and cerebral cortex but were increased (+186%) in midbrain. Mg levels were decreased (-12 to -32%) in pons and medulla, midbrain, and cerebellum. Fe levels were increased (+95%) in striatum but were decreased (-28%) in cerebral cortex. Cu levels were increased (+43 to +100%) in pons and medulla and striatum but Zn levels were decreased (-30%) in pons and medulla. Na levels were increased (+22%) in striatum but those of K and Cl remained unchanged. Type A monoamine oxidase activities were decreased (-13 to -16%) in midbrain, striatum, and cerebral cortex, but type B monoamine oxidase activities decreased (-13%) only in hypothalamus. Acetylcholinesterase activities were increased (+20 to +22%) in striatum and cerebellum. The results are consistent with our hypothesis that chronic manganese encephalopathy not only affects brain metabolism of Mn but also that of other metals.
Lai
J C
JC
Department of Biochemistry, Cornell University Medical College, New York, NY 10021.
Chan
A W
AW
Leung
T K
TK
Minski
M J
MJ
Lim
L
L
D000110:Acetylcholinesterase / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D001927:Brain Diseases / Q000201:enzymology / Q000378:metabolism*; D002712:Chlorides; D004573:Electrolytes / Q000378:metabolism; D008345:Manganese / Q000008:administration & dosage*; D017895:Manganese Compounds; D008995:Monoamine Oxidase / Q000187:drug effects / Q000378:metabolism; D009410:Nerve Degeneration / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D014131:Trace Elements / Q000378:metabolism
D002712:Chlorides; D004573:Electrolytes; D017895:Manganese Compounds; D014131:Trace Elements; D008345:Manganese; D008995:Monoamine Oxidase; D000110:Acetylcholinesterase; C025340:manganese chloride