Effect of hydrogen peroxide on mammalian chromatin: electron microscopic studies.
Chromatins purified from rat liver as well as from cultured Chinese hamster V79 cells were treated with hydrogen peroxide in presence of iron in vitro. When examined in a transmission electron microscope, chromatins from both the sources were found to lose the native morphology. There were evidences of aggregation of the chromatin fibers. This could be due to DNA-protein and DNA interstrand crosslinking induced by Fenton reaction.
Chaudhuri
S
S
Crystallography and Molecular Biology Division, Saha Institute of Nuclear Physics, Calcutta, India.
Sengupta
S
S
Bhattacharyya
N P
NP
Bhattacharjee
S B
SB
D000818:Animals; D002478:Cells, Cultured; D002843:Chromatin / Q000737:chemistry / Q000187:drug effects* / Q000302:isolation & purification / Q000648:ultrastructure*; D006224:Cricetinae; D003412:Cricetulus; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D008297:Male; D008854:Microscopy, Electron; D051381:Rats
D002843:Chromatin; D006861:Hydrogen Peroxide