Plasma assay of salbutamol by means of high-performance liquid chromatography with amperometric determination using a loop column for injection of plasma extracts. Application to the evaluation of subcutaneous administration of salbutamol.
An isocratic high-performance liquid chromatography method with amperometric detection for the assay of plasma salbutamol is described. The plasma extract is injected into the chromatographic system via a loop column. This insures the purification of the injected extracts and allows a simple and rapid liquid-solid extraction procedure. The good reliability, as shown by the low limit of detection (0.5 ng/ml) and a precision ranging between 5 and 10%, has permitted the investigation of a new mode of administration of salbutamol using a portable subcutaneous infusion pump. Our results show that subcutaneous administration yields plasma levels comparable with those obtained after usual intravenous doses.
Tamisier-Karolak
L
L
Laboratory of Pharmacokinetics and Toxicology, Ambroise Paré Hospital, Boulogne, France.
Delhotal-Landes
B
B
Jolliet-Riant
P
P
Milliez
J
J
Jannet
D
D
Barre
J
J
Flouvat
B
B
D000420:Albuterol / Q000008:administration & dosage / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D004563:Electrochemistry; D005260:Female; D005280:Fenoterol / Q000097:blood; D006801:Humans; D007260:Infusion Pumps; D007279:Injections, Subcutaneous; D011247:Pregnancy
D005280:Fenoterol; D000420:Albuterol