title: [Production of progesterone and testosterone in ovarian granulosa cells in sows after administration of nonapeptide hormones in vitro].
abstract: In the present paper the effects of nonapeptide hormones and of some of their chemical analogues were investigated on progesterone and testosterone production in granulosa cells of sow ovaries; the experiments were made in vitro. This objective was given by data on potential regulatory roles of nonapeptides at the level of hypothalamus, pituitary and reproductive organs. The goal of this experiment was to analyze the effects of various doses of oxytocin (OT), arginine-8-vasopressin (AVP), arginine-8-vasotocin and of some of their analogues on progesterone and testosterone production in vitro in granulosa cells of sow ovaries. The production activity of granulosa cells was investigated which were obtained from slaughtered sows without any changes in their reproductive process and abnormalities in their reproductive organs. Follicles of the size 2-5 mm without marked paleness in the early follicular phase were selected for aspiration. Granulosa cells with determined viability (more than 75%) and concentration (2 million/ml) were cultivated in defined culture conditions (37.5 degrees C, 5% CO2) after threefold resuspension and centrifugation of follicle fluid. These hormonal preparations were used in the experiments: pFSH, synthetic OT, synthetic AVP, synthetic AVP with antidiuretic effects and synthetic AVT. Progesterone and testosterone concentrations were analyzed radioimmunoanalytically using commercial kits of the Institute of Radio ecology and Nuclear Technology at Kosice. Statistically significant differences between the groups were evaluated by Student's t-test. The administered preparations were found to influence progesterone and testosterone production in dependence on the doses applied (Figs. 1-6). OT stimulation of progesterone production in granulosa cells indicated its regulatory role in relation to secretion of this hormone.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Výskumný ústav zivocísnej výroby, Nitra.
authors.forename: J
authors.initials: J
authors.lastname: Nitray
authors.forename: A
authors.initials: A
authors.lastname: Sirotkin
authors.forename: J
authors.initials: J
authors.lastname: Poltársky
mesh_terms: D000818:Animals; D001127:Arginine Vasopressin / Q000031:analogs & derivatives / Q000494:pharmacology*; D003894:Deamino Arginine Vasopressin / Q000494:pharmacology; D005260:Female; D006107:Granulosa Cells / Q000187:drug effects* / Q000378:metabolism*; D010121:Oxytocin / Q000031:analogs & derivatives / Q000494:pharmacology*; D011374:Progesterone / Q000096:biosynthesis*; D013552:Swine / Q000378:metabolism*; D013739:Testosterone / Q000096:biosynthesis*
chemical_list: D001127:Arginine Vasopressin; D013739:Testosterone; D011374:Progesterone; D010121:Oxytocin; D003894:Deamino Arginine Vasopressin
vernacular_title: Produkcia progesterónu a testosterónu bunkami granulózy vajecníkov osípaných po aplikácii nonapeptidových hormónov in vitro.