Nerve growth factor receptor gene expression in human peripheral blood lymphocytes in aging.
Nerve growth factor (NGF) has a modulating effect on immune function, which may occur as a consequence of binding to the NGF receptor (NGF-R). To determine if mRNA for the gene coding for p75NGFR (low affinity NGF-R) is present in lymphocytes, Northern blot analysis of mRNA from human peripheral blood lymphocytes (PBL) and purified T lymphocytes was initiated using cDNA probe for human p75NGFR. p75NGFR mRNA was present in PBL and T lymphocytes, and the mRNA in response to phytohemagglutinin stimulation showed maximum levels at 14 hr of stimulation. p75NGFR mRNA content when analyzed in PBL and T cells from volunteers of various ages showed that p75NGFR mRNA expression does not change with the age of the cell donor.
Kittur
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SD
Molecular Neurobiology Section, National Institute on Aging, Baltimore, Maryland 21224.
Song
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L
Endo
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Adler
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WH
D000328:Adult; D000368:Aged; D000375:Aging / Q000378:metabolism*; D015152:Blotting, Northern; D015342:DNA Probes; D015870:Gene Expression / Q000235:genetics*; D006801:Humans; D007700:Kinetics; D008214:Lymphocytes / Q000378:metabolism*; D008875:Middle Aged; D010835:Phytohemagglutinins; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D017475:Receptors, Nerve Growth Factor / Q000096:biosynthesis / Q000235:genetics*; D013601:T-Lymphocytes / Q000378:metabolism
D015342:DNA Probes; D010835:Phytohemagglutinins; D012333:RNA, Messenger; D017475:Receptors, Nerve Growth Factor