RASA contains a polymorphic microsatellite and maps to bovine syntenic group U22 on chromosome 7q2.4-qter.
The bovine gene for the p21ras protein activator (RASA) includes in its 5' untranslated region a (TG)n repeat. Analysis of this (TG)n repeat by PCR amplification of genomic DNA revealed a four-allele polymorphism. A cDNA probe was used to assign RASA to the region 2.4-qter of bovine Chromosome (Chr) 7 by in situ hybridization. PCR analysis of a panel of somatic hybrid lines allowed the assignment of RASA to the unassigned syntenic group 22 (U22) and thus localizes U22 on Chr 7.
Eggen
A
A
Department of Animal Science, Swiss Federal Institute of Technology, Zurich.
Solinas-Toldo
S
S
Dietz
A B
AB
Womack
J E
JE
Stranzinger
G
G
Fries
R
R
D000818:Animals; D001483:Base Sequence; D016466:CHO Cells; D002417:Cattle; D002871:Chromosome Banding; D002874:Chromosome Mapping; D006224:Cricetinae; D004276:DNA, Satellite / Q000235:genetics*; D006822:Hybrid Cells; D017403:In Situ Hybridization; D008297:Male; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D011506:Proteins / Q000235:genetics* / Q000378:metabolism; D016283:Proto-Oncogene Proteins p21(ras) / Q000378:metabolism
D004276:DNA, Satellite; D011506:Proteins; D016283:Proto-Oncogene Proteins p21(ras)