A new apheresis system using a copolymer (polyvinyl alcohol triethylene glycol diacrylate) for removal of LDL from dog whole blood.
Studies were undertaken to determine the effectiveness of a copolymer composed of PVA-TEGDA (Poly Vinyl Alcohol TriEthylene Glycol) as a plasma-cholesterol lowering procedure. For a comparative study, five dogs underwent plasmapheresis including the transfusion bag containing gel in the plasma line, while three control dogs underwent the same plasmapheresis without gel. Numerous biological determinations were performed firstly in whole blood circulation before and after treatment over 10 days, and secondly in plasma before and after LDL binding on the gel. In the whole blood circulation, the average significant depletion of cholesterol levels was 31-51% for treated, 0-16% for control dogs and the average significant depletion of LDL cholesterol was 26-75% for treated and 0-3% for control dogs. Gel was therefore able to bind 121-217 mg of total cholesterol and 34-70 mg of LDL cholesterol per gram of gel. Lipid and lipoprotein levels rebounded 3-4 days after treatment. Adverse effects were not observed during all plasmapheresis. No significant differences between control plasmapheresis and gel-plasmapheresis were obtained for blood cell counts while lengthened coagulation times were observed during 24 h. Complement was not significantly activated by the copolymer as shown by a same decreased activity in the blood stream of all dogs: in fact, CH 50 depletion in the gel incubated plasma was due to a protein adsorption on the hydrogel. This new approach for LDL apheresis appears to be a promising new technique.
Hugou
I
I
INSERM-U. 306, Université de Bordeaux II, France.
Janvier
G
G
Bordenave
L
L
Parmentier
F
F
Higueret
D
D
Cristol
G
G
Baquey
C
C
D000180:Acrylic Resins; D000818:Animals; D001798:Blood Proteins / Q000378:metabolism; D008078:Cholesterol, LDL / Q000302:isolation & purification*; D015941:Complement Hemolytic Activity Assay; D004285:Dogs; D005260:Female; D005782:Gels; D006403:Hematologic Tests; D006801:Humans; D008055:Lipids / Q000097:blood; D008074:Lipoproteins / Q000097:blood; D008422:Materials Testing; D010956:Plasmapheresis / Q000295:instrumentation*; D011092:Polyethylene Glycols; D011145:Polyvinyls
D000180:Acrylic Resins; D001798:Blood Proteins; D008078:Cholesterol, LDL; D005782:Gels; D008055:Lipids; D008074:Lipoproteins; D011145:Polyvinyls; C077598:polyvinyl alcohol-triethylene glycol diacrylate copolymer; D011092:Polyethylene Glycols