Solid-state stability studies of 13-cis-retinoic acid and all-trans-retinoic acid using microcalorimetry and HPLC analysis.
The solid-state stabilities of 13-cis-retinoic acid and all-trans-retinoic acid in the presence and absence of oxygen were investigated. The samples were first evaluated using microcalorimetry. The rate laws of different samples under different conditions were deduced from the shapes of the heat flow curves, and the activation energies of the reactions were determined from Arrhenius plots. Under an air atmosphere, the decomposition of 13-cis-retinoic acid is an autocatalytic reaction, while all-trans-retinoic acid undergoes a zero-order process. The degradation of the two compounds at a selected elevated temperature was also determined utilizing HPLC analysis. This technique confirmed the decomposition kinetics. Hence, their half-lives and shelf lives at room temperature could be calculated. Under a nitrogen atmosphere, the microcalorimetric experiment showed a first-order phenomenon for both samples, but HPLC analysis showed no degradation, suggesting that the two samples, in the absence of oxygen, undergo only a physical change.
Tan
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Department of Pharmaceutical Chemistry, University of Kansas, Lawrence 66045.
Meltzer
N
N
Lindebaum
S
S
D000388:Air; D002151:Calorimetry; D002851:Chromatography, High Pressure Liquid; D004355:Drug Stability; D015474:Isotretinoin / Q000737:chemistry*; D007700:Kinetics; D009584:Nitrogen; D013816:Thermodynamics; D014212:Tretinoin / Q000737:chemistry*
D014212:Tretinoin; D015474:Isotretinoin; D009584:Nitrogen