title: Mutations in the sup-38 gene of Caenorhabditis elegans suppress muscle-attachment defects in unc-52 mutants.
abstract: Mutations in the unc-52 locus of Caenorhabditis elegans have been classified into three different groups based on their complex pattern of complementation. These mutations result in progressive paralysis (class 1 mutations) or in lethality (class 2 and 3 mutations). The paralysis exhibited by animals carrying class 1 mutations is caused by disruption of the myofilaments at their points of attachment to the cell membrane in the body wall muscle cells. We have determined that mutations of this class also have an effect on the somatic gonad, and this may be due to a similar disruption in the myoepithelial sheath cells of the uterus, or in the uterine muscle cells. Mutations that suppress the body wall muscle defects of the class 1 unc-52 mutations have been isolated, and they define a new locus, sup-38. Only the muscle disorganization of the Unc-52 mutants is suppressed; the gonad abnormalities are not, and the suppressors do not rescue the lethal phenotype of the class 2 and class 3 mutations. The suppressor mutations on their own exhibit a variable degree of gonad and muscle disorganization. Putative null sup-38 mutations cause maternal-effect lethality which is rescued by a wild-type copy of the locus in the zygote. These loss-of-function mutations have no effect on the body wall muscle structure.
authors.affiliation: Department of Zoology, University of British Columbia, Vancouver, Canada.
authors.forename: E J
authors.initials: EJ
authors.lastname: Gilchrist
authors.forename: D G
authors.initials: DG
authors.lastname: Moerman
mesh_terms: D000483:Alleles; D000818:Animals; D017173:Caenorhabditis elegans / Q000033:anatomy & histology / Q000235:genetics*; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D005260:Female; D016153:Genes, Suppressor; D005816:Genetic Complementation Test; D008297:Male; D009132:Muscles / Q000002:abnormalities; D009154:Mutation; D010641:Phenotype