Light-induced fluorescence spectroscopy of adenomas, adenocarcinomas and non-neoplastic mucosa in human colon. I. In vitro measurements.
In an attempt to evaluate whether induced fluorescence could be exploited to discriminate neoplastic from non-neoplastic tissue, fluorescence spectroscopy was performed at 450-800 nm on 83 biopsy specimens of colonic mucosa. Measurements showed that fluorescence spectra of adenoma, adenocarcinoma and non-neoplastic mucosa manifest dissimilar patterns. Nine variables, whose photophysical and/or biological bases need further investigation, were derived from the spectra. Discriminant functions between the groups of lesions were determined by using a stepwise discriminant analysis. The diagnostic test had a sensitivity of 80.6% and 88.2%, and a specificity of 90.5% and 95.2% in discriminating neoplastic from non-neoplastic mucosa and adenoma from non-neoplastic mucosa respectively. These results suggest that fluorescence spectroscopy has the potential to improve endoscopic diagnosis of premalignant and malignant lesions of colonic mucosa.
Marchesini
R
R
Division of Health Physics, Istituto Nazionale Tumori, Milan, Italy.
Brambilla
M
M
Pignoli
E
E
Bottiroli
G
G
Croce
A C
AC
Dal Fante
M
M
Spinelli
P
P
di Palma
S
S
D000230:Adenocarcinoma / Q000473:pathology*; D000236:Adenoma / Q000473:pathology*; D001706:Biopsy; D003106:Colon / Q000166:cytology / Q000473:pathology; D003110:Colonic Neoplasms / Q000473:pathology*; D006801:Humans; D007413:Intestinal Mucosa / Q000166:cytology* / Q000473:pathology; D008027:Light; D013050:Spectrometry, Fluorescence / Q000295:instrumentation / Q000379:methods*