Yeast-specific DNA probes and their application for the detection of Candida albicans.
Two DNA fragments cloned from the genome of Candida albicans ATCC 10261 may be useful in the rapid diagnosis of disseminated candidosis. One sequence (probe EOB1) was specific for C. albicans (positive hybridisation with 45 strains tested). The second sequence (probe EOB2) detected C. albicans, as well as five other pathogenic Candida spp. and Saccharomyces cerevisiae, but did not react with human or bacterial DNA. Both probes were repetitive sequences in the genome of C. albicans. Probe EOB1 was used to detect, without DNA amplification, 500 C. albicans yeast cells in 1 ml of human blood.
Holmes
A R
AR
Department of Oral Biology and Pathology, School of Dentistry, University of Otago, Dunedin, New Zealand.
Lee
Y C
YC
Cannon
R D
RD
Jenkinson
H F
HF
Shepherd
M G
MG
D002176:Candida albicans / Q000235:genetics / Q000302:isolation & purification*; D002177:Candidiasis / Q000175:diagnosis*; D015342:DNA Probes; D004271:DNA, Fungal / Q000032:analysis; D006801:Humans; D015151:Immunoblotting; D009693:Nucleic Acid Hybridization; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D012680:Sensitivity and Specificity; D013045:Species Specificity
D015342:DNA Probes; D004271:DNA, Fungal