title: Release of granulocyte-macrophage colony-stimulating factor by alveolar macrophages in the lung of HIV-1-infected patients. A mechanism accounting for macrophage and neutrophil accumulation.
abstract: In this paper, the release of granulocyte-macrophage colony-stimulating factor (GM-CSF) in the lung of patients with HIV-1 infection was evaluated. This cytokine has well recognized effects on granulocyte and macrophage growth and differentiation and plays some role in the mechanisms leading to the accumulation of alveolar macrophages (AM) in patients with interstitial lung disease. Detectable levels of GM-CSF (up to 10 pg/ml) were demonstrated in unconcentrated bronchoalveolar lavage fluid retrieved from HIV-1-seropositive patients, thus suggesting that the GM-CSF is released in vivo in the lung during HIV-1 infection. A statistically significant correlation was demonstrated between the bronchoalveolar lavage concentrations of GM-CSF and the absolute numbers of AM and lung neutrophils. Cell-free supernatants obtained from unstimulated 24-h cultured AM isolated from HIV-1-infected patients contained discrete amounts of GM-CSF, as demonstrated by an immunoenzymatic assay. AM lost the capability of releasing GM-CSF after 72 h of culture, thus suggesting that the production of GM-CSF is not constitutive in AM. After exposition of AM with LPS, the release of GM-CSF and the expression of its mRNA significantly increased with respect to the baseline values; interestingly, the amount of GM-CSF released by LPS-stimulated AM was more than 10-fold higher in HIV-1-infected patients than in healthy subjects. As demonstrated by flow cytometry analysis, more than 70% of freshly isolated AM efficiently bound phycoerythrin-GM-CSF, thus indicating that they express the receptor for GM-CSF. Determination of AM in G1, S, and G2+M by flow cytometry showed that, after 48 h of culture with GM-CSF, 5.5 to 7% of AM entered the proliferative phase of the cell cycle. Taken together, these findings suggest that AM might represent an important source of GM-CSF production in HIV-1 infection. In particular, the hypothesis is formulated that pulmonary opportunists might trigger AM to synthesize GM-CSF in situ. The local overproduction of this cytokine is likely to play a role in the pathogenic events leading to the local proliferation of AM and recruitment of neutrophils in AIDS-associated interstitial lung disease.
authors.affiliation: Padua University School of Medicine, Department of Clinical Medicine, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Agostini
authors.forename: L
authors.initials: L
authors.lastname: Trentin
authors.forename: R
authors.initials: R
authors.lastname: Zambello
authors.forename: P
authors.initials: P
authors.lastname: Bulian
authors.forename: C
authors.initials: C
authors.lastname: Caenazzo
authors.forename: A
authors.initials: A
authors.lastname: Cipriani
authors.forename: P
authors.initials: P
authors.lastname: Cadrobbi
authors.forename: S
authors.initials: S
authors.lastname: Garbisa
authors.forename: G
authors.initials: G
authors.lastname: Semenzato
mesh_terms: D000386:AIDS-Related Complex / Q000473:pathology; D000328:Adult; D002455:Cell Division; D005260:Female; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000096:biosynthesis* / Q000235:genetics; D015658:HIV Infections / Q000473:pathology / Q000503:physiopathology*; D015497:HIV-1; D006801:Humans; D008171:Lung Diseases / Q000473:pathology; D016676:Macrophages, Alveolar / Q000502:physiology*; D008297:Male; D009504:Neutrophils / Q000502:physiology*; D012333:RNA, Messenger / Q000032:analysis; D016187:Receptors, Granulocyte-Macrophage Colony-Stimulating Factor / Q000032:analysis
chemical_list: D012333:RNA, Messenger; D016187:Receptors, Granulocyte-Macrophage Colony-Stimulating Factor; D016178:Granulocyte-Macrophage Colony-Stimulating Factor