Direct evidence for protein kinase C involvement in insulin-stimulated hexose uptake.
Insulin has been reported to translocate protein kinase C (PKC) in rat adipocytes, and activation of PKC by phorbol esters is known to increase hexose uptake in these cells (1.2). To test the hypothesis that PKC may participate in insulin-stimulated hexose uptake, adipocytes were partially depleted of protein kinase C by overnight phorbol ester treatment, thereby impairing insulin effects on hexose uptake. Purified PKC was then introduced into these PKC-depleted adipocytes by electropermeabilization, and this fully restored insulin-stimulated hexose uptake. These findings provide direct evidence that PKC is required for insulin-stimulated hexose uptake.
Cooper
D R
DR
Research Service, James A. Haley Veterans Administration Hospital, Tampa, FL 33612.
Watson
J E
JE
Hernandez
H
H
Yu
B
B
Standaert
M L
ML
Ways
D K
DK
Arnold
T T
TT
Ishizuka
T
T
Farese
R V
RV
D000273:Adipose Tissue / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002478:Cells, Cultured; D003847:Deoxyglucose / Q000378:metabolism*; D004558:Electric Stimulation; D005990:Glycerol / Q000378:metabolism; D008297:Male; D009004:Monosaccharide Transport Proteins / Q000378:metabolism*; D011493:Protein Kinase C / Q000378:metabolism*; D051381:Rats; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D009004:Monosaccharide Transport Proteins; D003847:Deoxyglucose; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate; D005990:Glycerol