Effects of S14001 on adrenergic neuroeffector interaction in isolated canine saphenous veins.
1. The effects of (S) fluoro-6 (morpholinyl-2 methoxy)-8-tetrahydro-1,2,3,4 quinoleine (S14001) on adrenergic neurotransmission in isolated canine saphenous veins were investigated in experiments which measured the accumulation, overflow and metabolism of 3H-norepinephrine. 2. S14001 inhibited the accumulation of total tritium (3H-norepinephrine and 3H-metabolites of norepinephrine) in a concentration-dependent manner. 3. Under basal conditions, S14001 increased tension and basal effiux of total tritium; the latter consisted predominantly of 3H-DOPEG. The increases in these parameters were not inhibited by desmethylimipramine (DMI). 4. During electrical stimulation, S14001 increased the contraction and overflow of total tritium; the latter consisted predominantly of 3H-DOPEG. The increases in these parameters were inhibited by DMI. 5. These experiments suggest that S14001 has dual effects on adrenergic neurotransmission in the canine saphenous vein: (a) an inhibitory action on the neuronal accumulation; and (b) a pharmacological displacement of the transmitter from adrenergic nerve terminals.
Hoshino
Y
Y
Department of Medicine, Baylor College of Medicine, Houston, Texas.
Verbeuren
T J
TJ
Hughes
H
H
Vanhoutte
P M
PM
D000818:Animals; D000928:Antidepressive Agents / Q000494:pharmacology*; D003891:Desipramine / Q000494:pharmacology; D004285:Dogs; D004558:Electric Stimulation; D007537:Isometric Contraction / Q000187:drug effects; D009025:Morpholines / Q000494:pharmacology*; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000294:innervation; D009451:Neuroeffector Junction / Q000187:drug effects* / Q000502:physiology; D009638:Norepinephrine / Q000378:metabolism; D011804:Quinolines / Q000494:pharmacology*; D011941:Receptors, Adrenergic / Q000187:drug effects / Q000502:physiology; D012501:Saphenous Vein
D000928:Antidepressive Agents; D009025:Morpholines; D011804:Quinolines; D011941:Receptors, Adrenergic; C077690:S 14001; D003891:Desipramine; D009638:Norepinephrine