Changes in the lectin-binding of capillaries in rat salivary glands after streptozotocin-induced diabetes.
The lectin-horseradish peroxidase conjugate of GSA I-B4 (Griffonia simplicifolia isolectin) has some binding affinity with capillary walls in sections of the major salivary glands from normal rats. After inducing diabetes with streptozotocin a conspicuous increase in the staining intensity with GSA I-B4 was already evident in parotid capillaries at 3 weeks and this increase persisted at 3 and 6 months. In submandibular capillaries, however, an increased uptake of GSA I-B4 was evident only at 6 months after inducing diabetes. The reasons for these different time scales in the two glands are not known but the increased uptake of GSA I-B4, which is due to an increase in carbohydrate-containing sites with available terminal alpha-D-galactose, is considered to reflect a pathological change.
Garrett
J R
JR
Department of Oral Pathology, King's College School of Medicine and Dentistry, Rayne Institute, London, U.K.
Winston
D C
DC
Proctor
G B
GB
Schulte
B A
BA
Anderson
L C
LC
D000818:Animals; D002196:Capillaries / Q000378:metabolism / Q000473:pathology; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism* / Q000473:pathology; D004848:Epithelium / Q000378:metabolism / Q000473:pathology; D005690:Galactose / Q000378:metabolism; D037102:Lectins / Q000378:metabolism*; D008297:Male; D037121:Plant Lectins; D051381:Rats; D017208:Rats, Wistar; D012469:Salivary Glands / Q000098:blood supply* / Q000378:metabolism / Q000473:pathology; D013045:Species Specificity; D013997:Time Factors
C045475:Griffonia simplicifolia lectins; D037102:Lectins; D037121:Plant Lectins; D005690:Galactose