[Physico-chemical, immunochemical, and biological activity of prolactin from human amniotic fluid].
Highly purified preparation of prolactin with molecular mass 23 kDa, which is the main form of the hormone in human amnionic fluid, was isolated from the fluid using gel filtration on Sephadex G-100, chromatography on DEAE-Sepharose, concanavalin A Sepharose, CM-cellulose. Physico-chemical, immunochemical and biological properties of the amnionic prolactin were studied as compared with those of hypophyseal hormone. Properties of the hormonal forms isolated from both these tissues were similar. The glycosylated form of prolactin with molecular mass 25.0 kDa was detected either in amnionic fluid or in hypophysis.
Bulatov
A A
AA
Makarovskaia
E E
EE
Seganova
M I
MI
Kuznetsov
V P
VP
D000596:Amino Acids / Q000032:analysis; D000653:Amniotic Fluid / Q000737:chemistry*; D015153:Blotting, Western; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006031:Glycosylation; D006801:Humans; D007120:Immunochemistry; D010902:Pituitary Gland / Q000737:chemistry; D011247:Pregnancy; D011388:Prolactin / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*
D000596:Amino Acids; D011388:Prolactin
Fiziko-khimicheskaia, immunokhimicheskaia i biologicheskaia kharakteristika prolaktina amnioticheskoĭ zhidkosti cheloveka.