title: Interleukin 6 modulation of second messenger systems in anterior pituitary cells.
abstract: We investigated the effect of interleukin-6 (IL-6) on second messenger systems in anterior pituitary (AP) cells. The acute exposition of membranes derived from the pituitary gland to IL-6 did not modify basal and forskolin-stimulated adenylate cyclase (AC) activity, as well as inositol phosphate (IP) production and free [Ca(++)]i. Preincubation of AP cells with IL-6 for 20 min did not affect basal second messengers levels, while completely abolished the stimulation by VIP of AC activity, partially inhibited forskolin-stimulated cAMP formation and reduced TRH-stimulated IP production. Finally, the pretreatment of AP cells for 20 min with IL-6 also reduced the TRH-induced rise in free [Ca(++)]i.
authors.affiliation: Dip. di Scienza delle Comunicazioni Umane, II Facoltà di Medicina e Chirurgia, Università di Napoli, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Grimaldi
authors.forename: O
authors.initials: O
authors.lastname: Meucci
authors.forename: A
authors.initials: A
authors.lastname: Scorziello
authors.forename: T
authors.initials: T
authors.lastname: Florio
authors.forename: C
authors.initials: C
authors.lastname: Ventra
authors.forename: R
authors.initials: R
authors.lastname: De Mercato
authors.forename: G
authors.initials: G
authors.lastname: Schettini
mesh_terms: D000262:Adenylyl Cyclases / Q000378:metabolism; D000704:Analysis of Variance; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D005260:Female; D007295:Inositol Phosphates / Q000378:metabolism; D015850:Interleukin-6 / Q000502:physiology*; D010903:Pituitary Gland, Anterior / Q000166:cytology / Q000201:enzymology / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D015290:Second Messenger Systems / Q000502:physiology*; D013997:Time Factors
chemical_list: D007295:Inositol Phosphates; D015850:Interleukin-6; D000262:Adenylyl Cyclases; D002118:Calcium