title: C-fos and c-myc modulation, mitogenic effect and Ia expression in the P388D1 murine macrophage line treated by immunomodifiers.
abstract: The aim of the present study was to investigate whether the early modulation of the c-fos and c-myc oncogenes could give some orientation to the impact of immunomodulators on the monocyte-macrophage lineage. In order to work in a homogeneous system we used the P388D1 mouse macrophage cell-line which is considered as an almost mature macrophage. When P388D1 cells were stimulated by LPS, interferon-gamma or the association of both compounds, no direct correlation could be found between the modulation of DNA synthesis and the early expression of the c-fos and c-myc oncogenes. The positive regulation of Ia antigen expression seemed to correlate with the absence of induction of c-fos oncogene.
authors.affiliation: Lab. d'Histologie, Cytogénétique, Faculté de Médecine Cochin-Port-Royal, Paris, France.
authors.forename: M M
authors.initials: MM
authors.lastname: Teyssier
authors.forename: Y C
authors.initials: YC
authors.lastname: Le Garrec
authors.forename: E H
authors.initials: EH
authors.lastname: Jullian
authors.forename: P J
authors.initials: PJ
authors.lastname: Michel
authors.forename: A J
authors.initials: AJ
authors.lastname: Pompidou
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D004261:DNA Replication / Q000187:drug effects; D004731:Endotoxins / Q000494:pharmacology*; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D005802:Genes, MHC Class II / Q000187:drug effects*; D016762:Genes, fos / Q000187:drug effects*; D016259:Genes, myc / Q000187:drug effects*; D007371:Interferon-gamma / Q000494:pharmacology*; D007941:Leukemia P388 / Q000235:genetics / Q000473:pathology*; D008070:Lipopolysaccharides; D008262:Macrophage Activation / Q000187:drug effects*; D051379:Mice; D011994:Recombinant Proteins; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000378:metabolism
chemical_list: D000276:Adjuvants, Immunologic; D004731:Endotoxins; D008070:Lipopolysaccharides; D011994:Recombinant Proteins; C044101:endotoxin, Escherichia coli; D007371:Interferon-gamma; D013755:Tetradecanoylphorbol Acetate