title: The effects of near-UV radiation on elasmobranch lens cytoskeletal actin.
abstract: The role of near-UV radiation as a cytoskeletal actin-damaging agent was investigated. Two procedures were used to analyse fresh smooth dogfish (Mustelus canis) eye lenses that were incubated for up to 22 hr in vitro, with elasmobranch Ringer's medium, and with or without exposure to a near-UV lamp (emission principally at 365 nm; irradiance of 2.5 mW cm-2). These were observed histologically using phalloidin-rhodamine specific staining and by transmission electron microscopy. In addition, solutions of purified polymerized rabbit muscle actin were exposed to the same UV conditions and depolymerization was assayed by ultracentrifugation and high-pressure liquid chromatography. While the two actins studied do differ very slightly in some amino acid sequences, they would react physically nearly identically. The results showed that dogfish lenses developed superficial opacities due to near-UV exposure. Whole mounts of lens epithelium exhibited breakdown of actin filaments in the basal region of the cells within 18 hr of UV exposure. TEM confirmed the breakdown of actin filaments due to UV exposure. SDS-PAGE and immunoblotting positively identified actin in these cells. Direct exposure of purified polymerized muscle actin in polymerizing buffer led to an increase in actin monomer of approximately 25% in the UV-exposed solutions within 3-18 hr, whether assayed by ultracentrifugation or HPLC. The above indicates that elasmobranch lens epithelial cells contain UV-labile actin filaments, and that near-UV radiation, as is present in the sunlit environment, can break down the actin structure in these cells. Furthermore, breakdown of purified polymerized muscle actin does occur due to near-UV light exposure.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Ophthalmology Research Laboratory, University of Rochester School of Medicine and Dentistry, NY 14642.
authors.forename: S
authors.initials: S
authors.lastname: Zigman
authors.forename: N S
authors.initials: NS
authors.lastname: Rafferty
authors.forename: D L
authors.initials: DL
authors.lastname: Scholz
authors.forename: K
authors.initials: K
authors.lastname: Lowe
mesh_terms: D000199:Actins / Q000032:analysis / Q000528:radiation effects* / Q000648:ultrastructure; D000818:Animals; D002386:Cataract / Q000209:etiology; D004284:Dogfish; D004591:Electrophoresis, Polyacrylamide Gel; D007908:Lens, Crystalline / Q000528:radiation effects* / Q000648:ultrastructure; D008854:Microscopy, Electron; D011817:Rabbits; D014466:Ultraviolet Rays
chemical_list: D000199:Actins