Characterization of the proteins from Melanoplus bivittatus that bind juvenile hormone, and a refined EFDA photolabeling technique.
1. Juvenile hormone (JH) is specifically bound by a protein from hemolymph and fat body cytosol of the grasshopper, Melanoplus bivittatus. 2. This protein has a native molecular weight of 331,000 and subunits of 77,000. 3. Proteins that bind JH were covalently photolabeled with a JH analog, epoxyfarnesyl diazoacetate (EFDA). Samples were irradiated in spot plates and hydroxyapatite was used to separate bound from free [3H]EFDA. Differential solubilization was used to extract unlinked [3H]EFDA and solubilize [3H]EFDA linked to protein. 4. Hemolymph proteins of M(r) 479,000, 240,000 and 77,000 also bound [3H]EFDA. 5. Proteins that bound [3H]EFDA were not vitellogenins.
Winder
B S
BS
Department of Entomology, University of California, Davis 95616.
Roberts
P E
PE
D000345:Affinity Labels; D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism; D003979:Diazonium Compounds; D015180:Electrophoresis, Gel, Two-Dimensional; D005204:Farnesol / Q000031:analogs & derivatives*; D005260:Female; D006110:Grasshoppers / Q000737:chemistry; D019476:Insect Proteins; D007605:Juvenile Hormones / Q000378:metabolism*
D000345:Affinity Labels; D002352:Carrier Proteins; D003979:Diazonium Compounds; D019476:Insect Proteins; D007605:Juvenile Hormones; C040772:epoxyfarnesyl diazoacetate; C035606:juvenile hormone-binding protein, insect; D005204:Farnesol