title: Percutaneous chemical gallbladder sclerosis after laser-induced cystic duct obliteration: results in an experimental model.
abstract: OBJECTIVE
  Chemical gallbladder sclerosis has been attempted as a way to defunctionalize the gallbladder in patients who have undergone nonsurgical removal of gallstones and who are unable to undergo surgical/laparoscopic cholecystectomy. The purpose of this investigation was threefold: to study an animal model for chemical sclerosis of the gallbladder with 95% ethanol and 3% sodium tetradecyl sulfate, to attempt chemical sclerosis immediately after percutaneous cystic duct obliteration by laser thermocoagulation, and to assess histopathologic changes in the gallbladder after sclerosis.
  
  
  METHODS
  Percutaneous cholecystostomy and laser thermocoagulation of the cystic duct was performed in 13 pigs. Eight pigs underwent immediate gallbladder sclerosis with 95% ethanol and 3% sodium tetradecyl sulfate while two pigs received 95% ethanol only. The remaining three pigs served as controls. The cholecystostomy catheter was removed immediately after the procedure. All animals were sacrificed 6 weeks after laser thermocoagulation. Multiple sections through the gallbladder, which included the adjacent liver, the cystic duct, and the common bile duct, were obtained for histologic examination.
  
  
  RESULTS
  At autopsy, the gallbladder in all 10 animals who underwent gallbladder sclerosis was reduced in size compared with controls. In all treated animals, the gallbladder mucosa was denuded; however, in nine of 10 cases reepithelialization had occurred. Complete sclerosis without reepithelialization was achieved in one pig who received both ethanol and sodium tetradecyl sulfate. In the two animals who received ethanol only, the depth of wall necrosis around the gallbladder lumen was less than in those pigs who received both ethanol and sodium tetradecyl sulfate. No pigs showed signs of hepatic necrosis or injury to the common bile duct.
  
  
  CONCLUSIONS
  Cystic duct laser thermocoagulation allows immediate gallbladder sclerotherapy without injury to the common bile duct. Sclerosis with ethanol and sodium tetradecyl sulfate results in denudation of the gallbladder mucosa. However, a single therapeutic session with immediate removal of the cholecystostomy catheter was inadequate for gallbladder ablation in this model because of reepithelialization.
authors.affiliation: Department of Radiology, Massachusetts General Hospital, Boston 02114.
authors.forename: M J
authors.initials: MJ
authors.lastname: Girard
authors.forename: S
authors.initials: S
authors.lastname: Saini
authors.forename: P R
authors.initials: PR
authors.lastname: Mueller
authors.forename: M J
authors.initials: MJ
authors.lastname: Lee
authors.forename: R E
authors.initials: RE
authors.lastname: Ribeiro
authors.forename: J T
authors.initials: JT
authors.lastname: Ferrucci
authors.forename: T J
authors.initials: TJ
authors.lastname: Flotte
mesh_terms: D000818:Animals; D002767:Cholecystostomy; D002769:Cholelithiasis / Q000517:prevention & control*; D003131:Combined Modality Therapy; D003549:Cystic Duct / Q000601:surgery*; D000431:Ethanol / Q000627:therapeutic use; D005240:Feasibility Studies; D005260:Female; D005704:Gallbladder / Q000187:drug effects* / Q000473:pathology; D017075:Laser Coagulation; D012008:Recurrence; D015911:Sclerotherapy; D012981:Sodium Tetradecyl Sulfate / Q000627:therapeutic use; D013552:Swine; D013997:Time Factors
chemical_list: D000431:Ethanol; D012981:Sodium Tetradecyl Sulfate