title: [A comparison of 3 methods of lipoprotein (a) isolation from human plasma].
abstract: Three methods for purification of lipoprotein (a) [Lp(a)] from human plasma were compared. Method I: two-stage ultracentrifugation with subsequent gel-filtration of Lp(a) containing fractions (1.063-1.090 g/ml) on Sepharose CL-4B. Method II: ultracentrifugation followed by affinity chromatography of plasma fraction (1.063 g/ml) on anti-apoB sorbent. Method III: affinity chromatography of the whole plasma on anti-apo(a) sorbent. The Lp(a) yield of these methods is 35, 54 and 41%, respectively. The method III is preferable of these three because it permitted high purification of a large amount of Lp(a) by single-step chromatography.
authors.forename: O I
authors.initials: OI
authors.lastname: Afanas'eva
authors.forename: I Iu
authors.initials: IIu
authors.lastname: Adamova
authors.forename: G F
authors.initials: GF
authors.lastname: Benevodenskaia
authors.forename: S N
authors.initials: SN
authors.lastname: Pokrovskiĭ
mesh_terms: D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D006801:Humans; D007164:Immunosorbents; D017270:Lipoprotein(a) / Q000097:blood*; D010956:Plasmapheresis; D014461:Ultracentrifugation
chemical_list: D007164:Immunosorbents; D017270:Lipoprotein(a)
vernacular_title: Sravnenie trekh metodov vydeleniia lipoproteida (a) na plazmy krovi cheloveka.