A biphasic system for primary isolation of mycobacteria compared to solid medium and broth culture.
A biphasic culture system, the MB Check, was compared with conventional culture on Löwenstein-Jensen egg (LJ) solid medium and with Bactec broth culture for primary isolation of mycobacteria from clinical samples. A total of 104 mycobacterial isolates was detected from 985 samples examined by the three methods. The most sensitive primary isolation was with LJ culture and MB Check; these methods detected 93% and 87% of all positive cultures, respectively. MB Check allowed a somewhat more rapid detection than LJ culture. The presence of atypical mycobacteria was demonstrated most rapidly with the Bactec system.
Hoffner
S E
SE
Department of Bacteriology, National Bacteriological Laboratory, Stockholm, Sweden.
Haile
M
M
Källenius
G
G
D001431:Bacteriological Techniques; D001980:Bronchi / Q000382:microbiology; D003470:Culture Media; D006801:Humans; D015269:Mycobacterium avium Complex / Q000302:isolation & purification*; D009169:Mycobacterium tuberculosis / Q000302:isolation & purification*; D009170:Nontuberculous Mycobacteria / Q000302:isolation & purification*; D013183:Sputum / Q000382:microbiology; D013270:Stomach / Q000382:microbiology
D003470:Culture Media