title: High-performance liquid chromatography of oligosaccharide alditols and glycopeptides on a graphitized carbon column.
abstract: The chromatographic behaviour of oligosaccharide alditols and glycopeptides containing neutral and acetamido sugars and sialic acid has been investigated on a HyperCarb porous graphitised carbon column. The alditols were substantially retained and could be eluted in 0-25% acetonitrile-0.05% trifluoroacetic acid in 0.05% aqueous trifluoroacetic acid between 3-30 min for mono- to hexasaccharides. Elution patterns were based on both size, charge and linkage such that isomeric compounds could be separated from each other.
authors.affiliation: Glycoconjugates Section, Clinical Research Centre, Harrow, Middlesex, UK.
authors.forename: M
authors.initials: M
authors.lastname: Davies
authors.forename: K D
authors.initials: KD
authors.lastname: Smith
authors.forename: A M
authors.initials: AM
authors.lastname: Harbin
authors.forename: E F
authors.initials: EF
authors.lastname: Hounsell
mesh_terms: D000595:Amino Acid Sequence; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006020:Glycopeptides / Q000737:chemistry / Q000302:isolation & purification*; D006108:Graphite; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry / Q000302:isolation & purification*; D013402:Sugar Alcohols / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D006020:Glycopeptides; D009844:Oligosaccharides; D013402:Sugar Alcohols; D006108:Graphite