title: Specificity of Amaranthus leucocarpus lectin.
abstract: We have demonstrated that Amaranthus leucocarpus lectin hemagglutinating activity was powerfully inhibited by the T-antigen, containing Gal(beta 1-3)GalNAc(alpha 1-3)Ser/Thr, and the Tn-antigen, which contains GalNAc(alpha 1-3)Ser/Thr. This suggests that the acetamido group at C-2 and the axial -OH at C-4 of the N-acetyl-D-galactopyranosylamine ring are important for lectin binding. The hemagglutination assays also established that desialylated and Pronase-treated human type O erythrocytes with an M phenotype were better recognized than erythrocytes from all other blood groups. The recognition was dependent on pH and ionic strength.
authors.affiliation: Departamento Biología Experimental, Universidad Autónoma del Estado de Morelos, Cuernavaca, Mexico.
authors.forename: E
authors.initials: E
authors.lastname: Zenteno
authors.forename: R
authors.initials: R
authors.lastname: Lascurain
authors.forename: L F
authors.initials: LF
authors.lastname: Montaño
authors.forename: L
authors.initials: L
authors.lastname: Vazquez
authors.forename: H
authors.initials: H
authors.lastname: Debray
authors.forename: J
authors.initials: J
authors.lastname: Montreuil
mesh_terms: D000116:Acetylgalactosamine / Q000378:metabolism*; D001789:Blood Group Antigens; D050260:Carbohydrate Metabolism; D002240:Carbohydrate Sequence; D004912:Erythrocytes / Q000378:metabolism*; D006386:Hemagglutination Tests; D006801:Humans; D006863:Hydrogen-Ion Concentration; D037102:Lectins / Q000378:metabolism*; D008969:Molecular Sequence Data; D037121:Plant Lectins; D010944:Plants / Q000378:metabolism
chemical_list: D001789:Blood Group Antigens; D037102:Lectins; D037121:Plant Lectins; D000116:Acetylgalactosamine