Sequences responsible for efficient replication of simian immunodeficiency virus SIVMND in cells of the monocyte/macrophage lineage.
We determined the susceptibility of monocytic cell lines to infection with viral strains derived from two infectious clones of simian immunodeficiency virus isolated from a mandrill. One of the strains, which replicates poorly in T cell lines, was found to grow more rapidly than the other in these cells. The viral determinant for this property was genetically mapped within the env gene encoding a surface protein. Six amino acid substitutions identified appeared to be located outside of the domains corresponding to human immunodeficiency virus type 1 env functional domains such as the CD4-binding and V3 loop regions.
Sakai
H
H
Institute for Virus Research, Kyoto University, Japan.
Sakuragi
S
S
Sakuragi
J
J
Kawamura
M
M
Shibata
R
R
Adachi
A
A
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D015752:Genes, env / Q000235:genetics; D006801:Humans; D008264:Macrophages / Q000382:microbiology*; D008969:Molecular Sequence Data; D009000:Monocytes / Q000382:microbiology*; D009928:Organ Specificity; D012367:RNA, Viral / Q000235:genetics*; D015302:Simian Immunodeficiency Virus / Q000235:genetics* / Q000254:growth & development*; D014779:Virus Replication
D012367:RNA, Viral