Isolation and properties of a bacteriocin-producing Carnobacterium piscicola isolated from fish.
A facultative psychrotrophic lactic acid bacterium isolated from fresh fish was identified as Carnobacterium piscicola on the basis of carbohydrate utilization, G + C content and 16S RNA analysis. Its bacteriocin, designated carnocin UI49, is produced during the mid-exponential phase of growth at temperatures between 15 degrees C and 34 degrees C. Carnocin UI49 is active against a large number of closely-related lactic acid bacteria including carnobacteria, lactobacilli, pediococci and lactococci. Furthermore, the bacteriocin has a bactericidic mode of action which results in lysis of sensitive cells. Maximum bactericidal activity is observed at 34 degrees C with a decrease in activity down to 15 degrees C where it is completely abolished.
Stoffels
G
G
University of Iceland, Science Institute, Reykjavík.
Nes
I F
IF
Guthmundsdóttir
A
A
D000818:Animals; D001430:Bacteriocins / Q000096:biosynthesis* / Q000494:pharmacology; D001433:Bacteriolysis; D001483:Base Sequence; D003470:Culture Media; D005399:Fishes / Q000382:microbiology*; D006093:Gram-Positive Asporogenous Rods / Q000187:drug effects / Q000254:growth & development / Q000302:isolation & purification*; D006868:Hydrolysis; D007777:Lactobacillaceae / Q000187:drug effects / Q000254:growth & development / Q000302:isolation & purification; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data
D001430:Bacteriocins; D003470:Culture Media