C-fos and c-myc modulation, mitogenic effect and Ia expression in the P388D1 murine macrophage line treated by immunomodifiers.
The aim of the present study was to investigate whether the early modulation of the c-fos and c-myc oncogenes could give some orientation to the impact of immunomodulators on the monocyte-macrophage lineage. In order to work in a homogeneous system we used the P388D1 mouse macrophage cell-line which is considered as an almost mature macrophage. When P388D1 cells were stimulated by LPS, interferon-gamma or the association of both compounds, no direct correlation could be found between the modulation of DNA synthesis and the early expression of the c-fos and c-myc oncogenes. The positive regulation of Ia antigen expression seemed to correlate with the absence of induction of c-fos oncogene.
Teyssier
M M
MM
Lab. d'Histologie, Cytogénétique, Faculté de Médecine Cochin-Port-Royal, Paris, France.
Le Garrec
Y C
YC
Jullian
E H
EH
Michel
P J
PJ
Pompidou
A J
AJ
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D004261:DNA Replication / Q000187:drug effects; D004731:Endotoxins / Q000494:pharmacology*; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D005802:Genes, MHC Class II / Q000187:drug effects*; D016762:Genes, fos / Q000187:drug effects*; D016259:Genes, myc / Q000187:drug effects*; D007371:Interferon-gamma / Q000494:pharmacology*; D007941:Leukemia P388 / Q000235:genetics / Q000473:pathology*; D008070:Lipopolysaccharides; D008262:Macrophage Activation / Q000187:drug effects*; D051379:Mice; D011994:Recombinant Proteins; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000378:metabolism
D000276:Adjuvants, Immunologic; D004731:Endotoxins; D008070:Lipopolysaccharides; D011994:Recombinant Proteins; C044101:endotoxin, Escherichia coli; D007371:Interferon-gamma; D013755:Tetradecanoylphorbol Acetate