title: Influence of an endogenous lectin substrate on cultured dorsal root ganglion cells.
abstract: Dorsal root ganglion neurons normally contain a 14,500 molecular weight lactose-binding lectin, designated L-14. Although this lectin is developmentally regulated, and is localized to specific neurons as well as to specific areas of the rat spinal cord, its function in the nervous system is not known. In an effort to study the possible role of this lectin on peripheral neurons, they were dissociated and grown on substrates consisting of either L-14 or laminin, a molecule known to support neurite outgrowth. In contrast to the random distribution and fine neurites displayed by neurons on laminin, those growing on L-14 formed large aggregates with highly fasciculated neurite bundles. Experiments using plant lectins with sugar-binding specificity similar to that of L-14, as well as another endogenous rat lectin not present in neurons, resulted in essentially no neuronal attachment or neurite outgrowth. In addition, the effects induced by L-14 were not blocked by high concentrations of competing sugars, suggesting that it interacts with neurons by a domain distinct from its carbohydrate-binding site.
authors.affiliation: Department of Psychiatry, University of California, San Francisco.
authors.forename: R L
authors.initials: RL
authors.lastname: Outenreath
authors.forename: A L
authors.initials: AL
authors.lastname: Jones
mesh_terms: D000818:Animals; D001665:Binding Sites; D002448:Cell Adhesion; D002478:Cells, Cultured; D037161:Galectins; D005727:Ganglia, Spinal / Q000648:ultrastructure*; D006388:Hemagglutinins / Q000494:pharmacology*; D007797:Laminin / Q000494:pharmacology; D037102:Lectins / Q000494:pharmacology; D016501:Neurites / Q000502:physiology; D009474:Neurons / Q000648:ultrastructure; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D037161:Galectins; D006388:Hemagglutinins; D007797:Laminin; D037102:Lectins