Identification of 27-oxo-octacosanoic acid and heptacosane-1,27-dioic acid in Legionella pneumophila.
Two long-chain fatty acids, 27-oxo-octacosanoic acid (28:0(27-oxo)) and heptacosane-1,27-dioic acid (27:0-dioic) were identified for the first time in phenol-chloroform-petroleum ether extracts of Legionella pneumophila, indicating that they are constituents of lipopolysaccharide. The fatty acids were characterised by combined gas-liquid chromatography/mass spectrometry and proton nuclear magnetic resonance spectroscopy. Moreover, minor amounts of 29-oxo-triacontanoic (30:0(29-oxo)) acid and nonacosane-1,29-dioic acid (29:0-dioic) as well as 27-hydroxy-octacosanoic acid (28:0(27-OH)) were present in the phenol-chloroform-petroleum ether extract.
Moll
H
H
Forschungsinstitut Borstel, Institut für Experimentelle Biologie und Medizin, FRG.
Sonesson
A
A
Jantzen
E
E
Marre
R
R
Zähringer
U
U
D003998:Dicarboxylic Acids / Q000302:isolation & purification*; D005227:Fatty Acids / Q000302:isolation & purification; D008401:Gas Chromatography-Mass Spectrometry; D007651:Keto Acids / Q000302:isolation & purification*; D016952:Legionella pneumophila / Q000737:chemistry*; D008070:Lipopolysaccharides / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy
D003998:Dicarboxylic Acids; D005227:Fatty Acids; D007651:Keto Acids; D008070:Lipopolysaccharides; C077897:27-oxooctacosanoic acid; C077899:heptacosane-1,27-dioic acid