Specificity of Amaranthus leucocarpus lectin.
We have demonstrated that Amaranthus leucocarpus lectin hemagglutinating activity was powerfully inhibited by the T-antigen, containing Gal(beta 1-3)GalNAc(alpha 1-3)Ser/Thr, and the Tn-antigen, which contains GalNAc(alpha 1-3)Ser/Thr. This suggests that the acetamido group at C-2 and the axial -OH at C-4 of the N-acetyl-D-galactopyranosylamine ring are important for lectin binding. The hemagglutination assays also established that desialylated and Pronase-treated human type O erythrocytes with an M phenotype were better recognized than erythrocytes from all other blood groups. The recognition was dependent on pH and ionic strength.
Zenteno
E
E
Departamento Biología Experimental, Universidad Autónoma del Estado de Morelos, Cuernavaca, Mexico.
Lascurain
R
R
Montaño
L F
LF
Vazquez
L
L
Debray
H
H
Montreuil
J
J
D000116:Acetylgalactosamine / Q000378:metabolism*; D001789:Blood Group Antigens; D050260:Carbohydrate Metabolism; D002240:Carbohydrate Sequence; D004912:Erythrocytes / Q000378:metabolism*; D006386:Hemagglutination Tests; D006801:Humans; D006863:Hydrogen-Ion Concentration; D037102:Lectins / Q000378:metabolism*; D008969:Molecular Sequence Data; D037121:Plant Lectins; D010944:Plants / Q000378:metabolism
D001789:Blood Group Antigens; D037102:Lectins; D037121:Plant Lectins; D000116:Acetylgalactosamine