Production of a low molecular weight growth inhibitory factor by adenovirus 12-transformed cells.
Malignant rodent cells transformed by human adenovirus 12 produce a potent cell growth inhibitory factor. The cell growth inhibitory factor inhibits the growth of and DNA synthesis in normal fibroblasts in vitro. Extent of the production of the cell growth inhibitory factor appears to be proportional to that of the malignancy of the transformed cells. C57AT1-AB cells, an adenovirus 12-transformant of C57BL/6 mouse origin, are highly tumorigenic in the syngeneic and allogeneic mice. The cell growth inhibitory factor produced by these cells was characterized for the physicochemical properties; the cell growth-inhibitory activity was quantitatively recovered in the filtrates of YM-2 membrane (M(r) less than 1,000), resistant to the heat treatments at 56 degrees C for 30 min and 100 degrees C for 5 min, and extractable by ethyl acetate under acid-condition. These results suggest that the cell growth inhibitory factor may be lipid or oligopeptides.
Tsuji
T
T
Department of Biology, Faculty of Science, Niigata University, Japan.
Mori
K
K
Sugimoto
K
K
Hamada
C
C
Mori
K J
KJ
D000260:Adenoviruses, Human / Q000472:pathogenicity*; D000818:Animals; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D006131:Growth Inhibitors / Q000096:biosynthesis* / Q000737:chemistry; D066298:In Vitro Techniques; D051379:Mice; D008970:Molecular Weight; D051381:Rats
D006131:Growth Inhibitors; D004247:DNA