title: The Drosophila nuclear protein Bx42, which is found in many puffs on polytene chromosomes, is highly charged.
abstract: The Drosophila nuclear protein Bx42 is present in a set of transcriptionally active puffs on polytene chromosomes. cDNA clones coding for this protein were isolated from a lambda gt11 expression library. The two Bx42 transcripts are ubiquitously expressed and are already detectable in early stages of development. The corresponding genomic region, in 8C7-8, was isolated and sequenced. Both transcripts direct the production of the same basic, highly charged 547 amino acid protein with a calculated 61.1 kDa molecular weight.
authors.affiliation: Institut für Entwicklungsbiologie, Universität zu Köln, Federal Republic of Germany.
authors.forename: C
authors.initials: C
authors.lastname: Wieland
authors.forename: S
authors.initials: S
authors.lastname: Mann
authors.forename: H
authors.initials: H
authors.lastname: von Besser
authors.forename: H
authors.initials: H
authors.lastname: Saumweber
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002875:Chromosomes / Q000737:chemistry* / Q000648:ultrastructure; D003001:Cloning, Molecular; D004247:DNA; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000235:genetics* / Q000254:growth & development; D004563:Electrochemistry; D005786:Gene Expression Regulation; D051379:Mice; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000737:chemistry / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D015183:Restriction Mapping; D014158:Transcription, Genetic
chemical_list: D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D009687:Nuclear Proteins; C077753:Bx42 protein, Drosophila; D004247:DNA