The nucleotide sequence of the M RNA segment of tomato spotted wilt virus, a bunyavirus with two ambisense RNA segments.
The complete sequence of the tomato spotted wilt virus (TSWV) M RNA segment has been determined. The RNA is 4821 nucleotides long and has an ambisense coding strategy similar to that of the S RNA segment. The M RNA segment contains two open reading frames (ORFs), one in the viral sense which encodes a protein with a predicted size of 33.6K, and one in the viral complementary sense which encodes the precursor to the G1 and G2 glycoproteins, with a predicted size of 127.4K. Both ORFs are expressed via the synthesis of subgenomic mRNAs that possibly terminate at a stable hairpin structure, located in the intergenic region. The precursor for the glycoproteins contains a sequence motif (RGD) which is characteristic of cellular attachment domains. Significant sequence homology was found between the G1 glycoproteins of members of the genus Bunyavirus and a corresponding region in the glycoprotein precursor of TSWV, indicating a close evolutionary relationship between these viruses. With the elucidation of the M RNA sequence, the complete nucleotide sequence of TSWV has been determined. TSWV represents the first member of the Bunyaviridae shown to contain two ambisense RNA segments.
Kormelink
R
R
Department of Virology, Wageningen, The Netherlands.
de Haan
P
P
Meurs
C
C
Peters
D
D
Goldbach
R
R
D000595:Amino Acid Sequence; D001483:Base Sequence; D002042:Bunyamwera virus / Q000235:genetics; D002043:Bunyaviridae / Q000235:genetics*; D003001:Cloning, Molecular; D016679:Genome, Viral; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010935:Plant Diseases / Q000382:microbiology; D010942:Plant Viruses / Q000235:genetics*; D010944:Plants / Q000382:microbiology; D010947:Plants, Toxic; D014176:Protein Biosynthesis; D017433:Protein Structure, Secondary; D012367:RNA, Viral / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D014026:Nicotiana / Q000382:microbiology; D014158:Transcription, Genetic; D014763:Viral Matrix Proteins / Q000235:genetics*
D012367:RNA, Viral; D014763:Viral Matrix Proteins