[Sequence-specific modification of nucleic acids by oligonucleotide derivative containing alkylating groups in the C-5-position of deoxyuridine].
A new type of alkylating derivatives of oligonucleotides with 4(N-methyl-N-2-chloroethylamino)benzyl (RCl) group at C-5 of deoxyuridine with a high extent of the target modification was prepared. The synthesized reagents d(ULNHRClCCACTT), where L = CH2 (Ia), CH2OCH2CH2 (Ib) and CH2NHCOCH2CH2 (Ic), proved to effectively (80-90%) modify the oligonucleotide d(TAAGTGGAGTTTGGC). The reagents (Ia) and (Ib) alkylate G6, G7 and G9 positions, while the reagent (Ic) modifies predominantly G9.
Zarytova
V F
VF
Levina
A S
AS
Khalimskaia
L M
LM
Sergeeva
Z A
ZA
D001483:Base Sequence; D003857:Deoxyuridine; D004586:Electrophoresis; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009696:Nucleic Acids / Q000737:chemistry*; D009841:Oligonucleotides
D009696:Nucleic Acids; D009841:Oligonucleotides; D003857:Deoxyuridine
Komplementarno adresovannaia modifikatsiia nukleinovykh kislot proizvodnymi oliognukleotidov, soderzhashchimi alkiliruiushchuiu gruppuv C-5-polozhenii dezoksiuridina.