title: Effect of insulin on the proline transport activity in cultured fibroblasts from patients with Werner syndrome.
abstract: The effect of insulin on the transport of proline has been studied in cultured fibroblasts from normal individuals, non-insulin-dependent diabetic patients, and patients with Werner syndrome. In fibroblasts from normal individuals and those with diabetes mellitus, incubation with 10(-7) M insulin resulted in more than a twofold increase in the transport rate after about 14 h incubation. In contrast, fibroblasts from patients with Werner syndrome had a markedly attenuated response to insulin, suggesting a defect in insulin action on the transport of this amino acid in Werner syndrome.
authors.affiliation: Department of Endocrinology and Metabolism, Tsukuba University Medical School, Ibaraki, Japan.
authors.forename: C
authors.initials: C
authors.lastname: Bannai
authors.forename: S
authors.initials: S
authors.lastname: Bannai
authors.forename: Y
authors.initials: Y
authors.lastname: Okuda
authors.forename: K
authors.initials: K
authors.lastname: Yamashita
mesh_terms: D000293:Adolescent; D000328:Adult; D001692:Biological Transport / Q000187:drug effects; D002478:Cells, Cultured; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism*; D005260:Female; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D008297:Male; D008875:Middle Aged; D011392:Proline / Q000378:metabolism*; D012016:Reference Values; D012867:Skin / Q000378:metabolism*; D014898:Werner Syndrome / Q000378:metabolism*
chemical_list: D011392:Proline