Protective effect of anisodamine on cultured bovine pulmonary endothelial cell injury induced by oxygen-free radicals.
Anisodamine, a Chinese traditional medicine herb, has been used for treatment of adult respiratory distress syndrome effectively, but little is known about its mechanism. We attempted to investigate if anisodamine could protect bovine pulmonary endothelial cell injury induced by exogenous oxygen-free radicals that were generated by xanthine/xanthine oxidase or opsonized zymosan-stimulated polymorphonuclear leukocytes. Results showed that with the addition of xanthine/xanthine oxidase into cultured bovine pulmonary endothelial cells, production of malondialdehyde and release of lactate dehydrogenase in supernatant increased, and synthesis of prostacyclin decreased. Damaged cellular membranes were revealed by scanning electron microscopy. The same was true for the addition of opsonized zymosan-stimulated polymorphonuclear leukocytes. While treatment with anisodamine greatly attenuated all of the above-mentioned parameters, results showed that (1) cultured bovine pulmonary endothelial cells could be damaged by oxygen-free radicals, (2) anisodamine had a protective effect on this injury as effective as that of superoxide dismutase and catalase, and (3) the membrane-stable action might contribute to the mechanism of protective effect against this injury.
Luo
Z Y
ZY
Department of Pathophysiology, Hunan Medical University, Changsha, People's Republic of China.
Tang
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Luo
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D015121:6-Ketoprostaglandin F1 alpha / Q000032:analysis; D000818:Animals; D002374:Catalase / Q000494:pharmacology; D002417:Cattle; D002478:Cells, Cultured; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000494:pharmacology*; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D005609:Free Radicals; D007770:L-Lactate Dehydrogenase / Q000032:analysis; D008315:Malondialdehyde / Q000032:analysis; D008855:Microscopy, Electron, Scanning; D009504:Neutrophils / Q000502:physiology; D010100:Oxygen / Q000009:adverse effects*; D011651:Pulmonary Artery / Q000166:cytology; D012991:Solanaceous Alkaloids / Q000008:administration & dosage / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000494:pharmacology; D019820:Xanthine; D014969:Xanthine Oxidase / Q000009:adverse effects; D014970:Xanthines / Q000009:adverse effects; D015054:Zymosan / Q000009:adverse effects
D004365:Drugs, Chinese Herbal; D005609:Free Radicals; D012991:Solanaceous Alkaloids; D014970:Xanthines; C003922:anisodamine; D019820:Xanthine; D008315:Malondialdehyde; D015121:6-Ketoprostaglandin F1 alpha; D015054:Zymosan; D007770:L-Lactate Dehydrogenase; D002374:Catalase; D013482:Superoxide Dismutase; D014969:Xanthine Oxidase; D010100:Oxygen