title: Evaluation of two ELISA's for detecting Chlamydia trachomatis from endocervical swabs.
abstract: Two enzyme immunoassays (EIAs) detecting Chlamydia trachomatis from endocervical swabs, Syva MicroTrak (MT) and Abbott Chlamydiazyme (CZ), were compared with a tissue culture (TC) standard. Initially, 8% (100 of 1250) of specimens were TC positive, yielding sensitivities of 94% (94 of 100) for MT and 79% (79 of 100) for CZ with identical 98% specificities (1129 of 1150 for MT and 1130 of 1150 for CZ). Discrepant specimens were retested by both EIAs and assayed for elementary bodies (EBs) by a fluorescent antibody test. After discrepancy analysis, 9.5% (118) of 1240 patients were either TC or EB positive, yielding sensitivities of 94.1% for MT (111 of 118) and 79.7% for CZ (94 of 118) with identical specificities of 100% (1122 of 1122). These results indicate that the MT is significantly more sensitive (p less than 0.05, McNemar test) than CZ in detecting C. trachomatis from endocervical swabs.
authors.affiliation: Department of Pediatrics, State University of New York, Buffalo School of Medicine and Biological Sciences.
authors.forename: S S
authors.initials: SS
authors.lastname: Altaie
authors.forename: F A
authors.initials: FA
authors.lastname: Meier
authors.forename: R M
authors.initials: RM
authors.lastname: Centor
authors.forename: M
authors.initials: M
authors.lastname: Wakabongo
authors.forename: D
authors.initials: D
authors.lastname: Toksoz
authors.forename: K M
authors.initials: KM
authors.lastname: Harvey
authors.forename: E
authors.initials: E
authors.lastname: Basinger
authors.forename: B A
authors.initials: BA
authors.lastname: Johnson
authors.forename: R R
authors.initials: RR
authors.lastname: Brookman
authors.forename: H P
authors.initials: HP
authors.lastname: Dalton
mesh_terms: D000293:Adolescent; D000328:Adult; D002648:Child; D002690:Chlamydia Infections / Q000175:diagnosis; D002692:Chlamydia trachomatis / Q000302:isolation & purification*; D046508:Culture Techniques / Q000592:standards; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005069:Evaluation Studies as Topic; D005188:False Negative Reactions; D005189:False Positive Reactions; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D012680:Sensitivity and Specificity; D014626:Vaginal Smears