title: Maltose uptake and its regulation in Bacillus subtilis.
abstract: Extracts prepared from cultures of Bacillus subtilis, grown on maltose as the sole carbon source, lacked maltose phosphotransferase system activity. There was, however, evidence for a maltose phosphorylase activity, and such extracts also possessed both glucokinase and glucose phosphotransferase system activities. Maltose was accumulated by whole cells of B. subtilis by an energy-dependent mechanism. This uptake was sensitive to the effects of uncouplers, suggesting a role for the proton-motive force in maltose transport. Accumulation of maltose was inhibited in the presence of glucose, and there was no accumulation of maltose by a strain carrying the ptsI6 null-mutation. A strain carrying the temperature-sensitive ptsI1 mutation accumulated maltose normally at 37 degrees C but, in contrast to the wild-type, was devoid of maltose transport activity at 47 degrees C. The results indicate a role for the phosphotransferase system in the regulation of maltose transport activity in this organism.
authors.affiliation: Department of Biological Sciences, Heriot-Watt University, Riccarton, Edinburgh, UK.
authors.forename: M
authors.initials: M
authors.lastname: Tangney
authors.forename: C J
authors.initials: CJ
authors.lastname: Buchanan
authors.forename: F G
authors.initials: FG
authors.lastname: Priest
authors.forename: W J
authors.initials: WJ
authors.lastname: Mitchell
mesh_terms: D001412:Bacillus subtilis / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D001693:Biological Transport, Active / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D005947:Glucose / Q000494:pharmacology; D005964:Glucosyltransferases / Q000378:metabolism; D008320:Maltose / Q000378:metabolism*; D009154:Mutation; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000378:metabolism; D014475:Uncoupling Agents / Q000494:pharmacology
chemical_list: D014475:Uncoupling Agents; D008320:Maltose; D005964:Glucosyltransferases; C021535:maltose phosphorylase; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System; D005947:Glucose