Viral susceptibility of an immortalized human microvascular endothelial cell line.
CDC/EU.HMEC-1 is the first immortalized human microvascular endothelial cell line that retains morphologic, phenotypic, and functional characteristics of a normal human microvascular endothelial cell. This study evaluates a variety of viruses and their effects on this human endothelial cell line. The data indicate that adenoviruses, some herpesviruses, reoviruses and most picornaviruses grow well in HMEC-1, with distinctive cytopathic effects. The paramyxoviruses, however, do not appear to propagate, nor does HIV. The findings indicate that microvascular endothelial cells may act as a reservoir of these viruses; it also suggests the possibility that microvascular endothelium could be involved in the processing and presentation of antigen to immune cells.
Ades
E W
EW
Biological Products Branch, Centers for Disease Control, Atlanta, Georgia 30333.
Hierholzer
J C
JC
George
V
V
Black
J
J
Candal
F
F
D000256:Adenoviridae / Q000254:growth & development / Q000472:pathogenicity; D002460:Cell Line; D003588:Cytopathogenic Effect, Viral; D004730:Endothelium, Vascular / Q000166:cytology / Q000382:microbiology*; D006678:HIV / Q000254:growth & development / Q000472:pathogenicity; D006564:Herpesviridae / Q000254:growth & development / Q000472:pathogenicity; D006801:Humans; D010252:Paramyxoviridae / Q000254:growth & development / Q000472:pathogenicity; D010849:Picornaviridae / Q000254:growth & development / Q000472:pathogenicity; D012087:Reoviridae / Q000254:growth & development / Q000472:pathogenicity; D014773:Virology / Q000379:methods; D014780:Viruses / Q000254:growth & development / Q000472:pathogenicity*