Quantitative autoradiographic mapping of a novel serotonin receptor-5-HT1S.
The spinal cord distribution of a recently identified, high affinity 3H-5-HT binding site, the 5-HT1S receptor was studied employing quantitative autoradiography with microdensitometry. The laminar distribution and density of other high affinity 3H-5-HT binding sites present in rat spinal cord, 5-HT1A, 5-HT1B and 5-HT1C, was also determined. The greatest density of 5-HT1S receptors was found in the superficial dorsal horn laminae, the autonomic nuclei around the central canal and the ventral motor nuclei. Smaller, variable densities of 5-HT1S receptors were observed in other spinal cord regions. A similar spatial pattern of receptor density was observed for 5-HT1A, 5-HT1B and 5-HT1C receptors. The distribution of 5-HT1S receptors suggests functional modulation of somatosensory input to spinal cord as well as control of visceral and somatomotor activity.
Murphy
R M
RM
Department of Psychiatry, University of Cincinnati College of Medicine, Ohio 45267-0559.
Zemlan
F P
FP
D017371:8-Hydroxy-2-(di-n-propylamino)tetralin / Q000494:pharmacology; D000818:Animals; D001345:Autoradiography; D008024:Ligands; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D011985:Receptors, Serotonin / Q000187:drug effects / Q000378:metabolism*; D013116:Spinal Cord / Q000033:anatomy & histology / Q000378:metabolism*; D013574:Synaptosomes / Q000378:metabolism
D008024:Ligands; D011985:Receptors, Serotonin; D017371:8-Hydroxy-2-(di-n-propylamino)tetralin