Effects of Phyllanthus plant extracts on duck hepatitis B virus in vitro and in vivo.
The effects of extracts of five Australian Phyllanthus species (P. hirtellus, P. gunnii, P. gasstroemii, P. similis and P. tenellus), other plant extracts and the antiviral drug foscarnet on duck hepatitis B virus (DHBV) endogenous DNA polymerase (DNAp) activity were compared. All 5 Phyllanthus species caused 50% inhibition at concentrations of dry weight between 350-800 micrograms/ml, which is comparable with the effect described for P. amarus on the DNAp of human and woodchuck hepatitis B viruses. Incubation of P. hirtellus with 100 ID50 DHBV neutralized infection. However, neither P. gasstroemi extract, given by intraperitoneal injection (i.p.) at a dose of 20 mg/kg 3 times per week to ducklings early in the incubation period, or P. hirtellus extract, given to established DHBV carrier ducklings, prevented or eliminated infection.
Shead
A
A
Department of Infectious Diseases, University of Sydney, Australia.
Vickery
K
K
Pajkos
A
A
Medhurst
R
R
Freiman
J
J
Dixon
R
R
Cossart
Y
Y
D000818:Animals; D000831:Animals, Newborn / Q000502:physiology; D000998:Antiviral Agents / Q000494:pharmacology* / Q000627:therapeutic use; D004372:Ducks / Q000382:microbiology*; D017245:Foscarnet / Q000494:pharmacology; D015611:Hepatitis B Virus, Duck / Q000187:drug effects* / Q000472:pathogenicity; D006524:Hepatitis, Viral, Animal / Q000188:drug therapy* / Q000382:microbiology; D019384:Nucleic Acid Synthesis Inhibitors; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use; D010946:Plants, Medicinal / Q000737:chemistry*; D011201:Poultry Diseases / Q000188:drug therapy* / Q000382:microbiology; D014779:Virus Replication / Q000187:drug effects
D000998:Antiviral Agents; D019384:Nucleic Acid Synthesis Inhibitors; D010936:Plant Extracts; D017245:Foscarnet