title: Stimulatory effects of ingenols from Euphorbia kansui on the expression of macrophage Fc receptor.
abstract: Immune complex binding to macrophages was enhanced by treatment with an E. kansui extract. Systematic fractionation of the extract led to the characterization of 3-O-(2'E,4'Z-decadienoyl)- and 3-O-(2,3-dimethylbutyryl)-13- O-n-dodecanoyl-13-hydroxyingenol as the active principles. Immune complex binding to macrophages by the action of these compounds increased in a dose-dependent manner. When each ingenol (10 nM) was added to the separated culture medium, the immune complex binding ability of macrophages increased up to 2-fold, respectively. Scatchard analysis showed the enhanced expression of the Fc-receptor for gamma-globulin by the action of each ingenol to macrophages. This Fc-receptor upregulation was dependent on RNA synthesis, suggesting a possible de novo synthesis.
authors.affiliation: Oriental Medicine Research Center, Kitasato Institute, Tokyo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Matsumoto
authors.forename: J C
authors.initials: JC
authors.lastname: Cyong
authors.forename: H
authors.initials: H
authors.lastname: Yamada
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004224:Diterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D008813:Mice, Inbred ICR; D015394:Molecular Structure; D010946:Plants, Medicinal / Q000737:chemistry*; D011961:Receptors, Fc / Q000096:biosynthesis*; D015854:Up-Regulation
chemical_list: D004224:Diterpenes; D011961:Receptors, Fc; C008125:ingenol