Atypical pyramidal cells in epileptic human cortex: CFLS and 3-D reconstructions.
Epileptic temporal cortices, removed from 3 patients with intractable partial epilepsy (IPE) during neurosurgery, were studied. Pyramidal neurons (40-50 per slice) in laminae III, V and white matter, were injected with lucifer yellow. Samples were examined in a confocal laser scanning microscope (Biorad 600) and individual cells scanned at 0.1-1 microns incremental levels. 2-D maximal linear projection was used for overview. Frames (50-60) of scanned neurons were transformed into 3-D volumes, using VoxelView software on a Silicone Graphics workstation and rotated. All samples contained neurons with duplicated apical dendrites, additional basal dendrites or were misplaced in a horizontal position in the white matter. The relation between these preliminary observations and the disease is discussed.
Belichencko
P
P
Brain Research Institute, Academy of Sciences, Russia.
Dahlström
A
A
von Essen
C
C
Lindström
S
S
Nordborg
C
C
Sourander
P
P
D004828:Epilepsies, Partial / Q000473:pathology*; D005456:Fluorescent Dyes; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007546:Isoquinolines; D007834:Lasers; D009474:Neurons / Q000648:ultrastructure; D013702:Temporal Lobe / Q000473:pathology* / Q000648:ultrastructure
D005456:Fluorescent Dyes; D007546:Isoquinolines; C017475:lucifer yellow