title: Analysis of the major surfactant-associated proteins in human lung.
abstract: The protein component of human pulmonary surfactant was analysed. A purified surfactant preparation, after delipidation, was subjected to gel filtration on Sephadex G-200. The proteins present in the surfactant were categorised by means of SDS-polyacrylamide gel electrophoresis into serum and non-serum components. Molecular masses determination showed the presence of three sub-groups with molecular masses of 60-68 kDa, 28-36 kDa and 10-18 kDa, respectively. Antiserum generated against 28-36 kDa protein strongly reacted with the purified surfactant and amniotic fluid, while it did not show any cross reactivity with other groups of proteins and serum in a double diffusion immunoprecipitation assay. We propose that this protein is the major non-serum surfactant-associated protein present in human lung surfactant.
authors.affiliation: Department of Obstetrics & Gynaecology, Postgraduate Institute of Medical Education & Research, Chandigarh.
authors.forename: A
authors.initials: A
authors.lastname: Wali
authors.forename: K
authors.initials: K
authors.lastname: Dhall
authors.forename: S N
authors.initials: SN
authors.lastname: Sanyal
authors.forename: R
authors.initials: R
authors.lastname: Juneja
authors.forename: S
authors.initials: S
authors.lastname: Majumdar
mesh_terms: D000328:Adult; D001798:Blood Proteins / Q000032:analysis / Q000276:immunology; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007106:Immune Sera; D008168:Lung / Q000737:chemistry*; D008970:Molecular Weight; D011233:Precipitin Tests; D011663:Pulmonary Surfactants / Q000032:analysis* / Q000276:immunology
chemical_list: D001798:Blood Proteins; D007106:Immune Sera; D011663:Pulmonary Surfactants