[Mutagenic effect on Escherichia coli bacteria of 8-hydroxy-2'-deoxyguanosine--a DNA base damage product induced by oxygen radicals and ionizing radiation].
The effect of 8-oxo-2'-deoxyguanosine (8-oxo-dG) (8-hydroxydeoxyguanosine)--a DNA base damage product induced by oxygen radicals and irradiation on survival and mutagenesis in Escherichia coli strains C-600 and P-687 was investigated. Survival and mutagenesis curves, in dependence of 8-oxo-dG concentrations in the medium, ranging from 0.2 through 10 mM, were obtained. Bacterial survival at all 8-oxo-dG concentrations tested was shown to be no lesser than in the control. The mutagenic effect of 8-oxo-dG was tested by frequency of reversions in the absence of leucine and threonine. A non-linear dependence of mutagenesis on the concentration was observed. Linear increase in the amount of revertants took place at concentrations of 8-oxo-dG lower than 1 mM, and being kept constant at higher concentrations. Induction of SOS repair under the action of 8-oxo-dG in E. coli PQ37 strain was estimated according to alteration of activity of beta-galactosidase in the SOS chromotest. Weak induction of the SOS response was observed within the wide range of 8-oxo-dG concentration values, which points to a lack of genotoxicity and independence of mutagenesis on SOS repair.
Bruskov
V I
VI
Iurov
S S
SS
Chernov
B K
BK
Bezlepkin
V G
VG
D000080242:8-Hydroxy-2'-Deoxyguanosine; D004249:DNA Damage; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000633:toxicity; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics*; D005609:Free Radicals; D005720:Gamma Rays; D009153:Mutagens / Q000633:toxicity*; D017382:Reactive Oxygen Species / Q000633:toxicity*; D013014:SOS Response, Genetics / Q000235:genetics; D001616:beta-Galactosidase / Q000378:metabolism
D005609:Free Radicals; D009153:Mutagens; D017382:Reactive Oxygen Species; D000080242:8-Hydroxy-2'-Deoxyguanosine; D001616:beta-Galactosidase; D003849:Deoxyguanosine
Mutagennoe deĭstvie na bakterii Escherichia coli 8-oksi-2'-dezoksiguanozina--produkta povrezhdeniia osnovaniĭ DNK pod vliianiem kislorodnykh radikalov i ioniziruiushchego izlucheniia.