A radiation hybrid map of the region on human chromosome 22 containing the neurofibromatosis type 2 locus.
We describe a high-resolution radiation hybrid map of the region on human chromosome 22 containing the neurofibromatosis type 2 (NF2) gene. Eighty-five hamster-human somatic cell hybrids generated by X-irradiation and cell fusion were used to generate the radiation hybrid map. The presence or absence of 18 human chromosome 22-specific markers was determined in each hybrid by using Southern blot hybridization. Sixteen of the 18 markers were distinguishable by X-ray breakage in the radiation hybrids. Analysis of these data using two different mathematical models and two different statistical methods resulted in a single framework map consisting of 8 markers ordered with odds greater than 1000:1. The remaining nonframework markers were all localized to regions consisting of two adjoining intervals on the framework map with odds greater than 1000:1. Based on the RH map, the NF2 region of chromosome 22, defined by the flanking markers D22S1 and D22S28, is estimated to span a physical distance of approximately 6 Mb and is the most likely location for 9 of the 18 markers studied: D22S33, D22S41, D22S42, D22S46, D22S56, LIF, D22S37, D22S44, and D22S15.
Frazer
K A
KA
Department of Anatomy, University of California, San Francisco 94143.
Boehnke
M
M
Budarf
M L
ML
Wolff
R K
RK
Emanuel
B S
BS
Myers
R M
RM
Cox
D R
DR
D000818:Animals; D002460:Cell Line; D002874:Chromosome Mapping; D002892:Chromosomes, Human, Pair 22; D006224:Cricetinae; D016515:Genes, Neurofibromatosis 2; D005819:Genetic Markers; D006801:Humans; D006822:Hybrid Cells; D008957:Models, Genetic; D011831:Radiation Genetics
D005819:Genetic Markers