[Cloning, primary structure determination and expression of preproinsulin cDNA from human insulinoma in Escherichia coli].
A human insulinoma cDNA library was constructed in expression plasmid vector pUEX1. Clone pUEX1Ins12 was selected from human insulinoma cDNA library by means of hybridization with the insulin probe and a nucleotide sequence of the insertion was determined. It codes for full size amino acid sequence preproinsulin and furthermore, contains the entire 3'-end of noncoding mRNA region and 44 nucleotides from the 5'-untranslated region. The bacterial strain pUEX3Ins8 producing preproinsulin as beta-galactosidase fusion protein was constructed.
Chekhranova
M K
MK
Shuvalova
E R
ER
Kutin
A M
AM
Butnev
V Iu
VIu
Valentsova
A B
AB
Il'ina
E N
EN
Pankov
Iu A
IuA
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA; D004926:Escherichia coli / Q000235:genetics*; D015870:Gene Expression; D006801:Humans; D007328:Insulin; D007340:Insulinoma / Q000378:metabolism*; D008969:Molecular Sequence Data; D010957:Plasmids; D011384:Proinsulin / Q000235:genetics*; D011498:Protein Precursors / Q000235:genetics*
D007328:Insulin; D011498:Protein Precursors; C013737:preproinsulin; D004247:DNA; D011384:Proinsulin
Klorirovanie, opredelenie pervichnoĭ struktury i ékspressiia kDNK preproinsulina iz insulinomy cheloveka v Escherichia coli.