title: Distortion of transmission ratio by a candidate t complex responder locus transgene.
abstract: The mouse t complex responder locus (Tcr) is centrally involved in the phenomenon of male-specific transmission ratio distortion (TRD) through its action in haploid germ cells. Previously, we identified a candidate gene, Tcp-10b, whose t allele generates alternatively spliced transcripts. The full-length Tcp-10bt transcript is present in pre- and postmeiotic germ cells and encodes a product that is virtually identical with that encoded by the wild-type allele. The alternatively spliced t-specific transcript is observed in post-meiotic haploid spermatids and would encode an altered polypeptide that could convey the Tcrt phenotype. To assess their function, we have introduced constructs representing each Tcp-10bt transcript into transgenic mice. Breeding experiments demonstrate that these two constructs alter the transmission ratios of t haplotypes from male mice, but in opposite directions. The results provide support for the hypothesis that Tcp-10bt is a component of the Tcr locus.
authors.affiliation: Department of Molecular Biology, Princeton University, NJ 08544-1014.
authors.forename: L C
authors.initials: LC
authors.lastname: Snyder
authors.forename: L M
authors.initials: LM
authors.lastname: Silver
mesh_terms: D000818:Animals; D001483:Base Sequence; D004277:DNA, Single-Stranded; D005260:Female; D015870:Gene Expression; D047908:Intracellular Signaling Peptides and Proteins; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D008822:Mice, Transgenic; D008869:Microtubule-Associated Proteins; D008957:Models, Genetic; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics*; D010641:Phenotype; D044767:Ubiquitin-Protein Ligases; D056427:t-Complex Genome Region
chemical_list: D004277:DNA, Single-Stranded; D047908:Intracellular Signaling Peptides and Proteins; D008869:Microtubule-Associated Proteins; D009687:Nuclear Proteins; C116196:PPP1R11 protein, human; D044767:Ubiquitin-Protein Ligases