Heterogeneity in restriction patterns of Gardnerella vaginalis isolates from individuals with bacterial vaginosis.
This study was undertaken to resolve the genetic make up of Gardnerella vaginalis present in bacterial vaginosis (BV). DNA from several G. vaginalis isolates from within and between individual BV patients were compared by BamHI, ClaI and EcoRI restriction endonuclease analysis (REA) followed by a restriction fragment length polymorphism (RFLP) study, utilizing a 5.7-kb BamHI G. vaginalis ATCC14018 DNA probe. Four G. vaginalis isolates from one patient (GVP-062) were composed of 3 different biotypes (biotypes 3, 5 and 8), and while the REA mirrored the biotype, in RFLP studies at least 3 isolates had DNA fragments in common. All of the isolates from 2 other patients (GVP-063 and GVP-072) represented a single biotype (biotype 2), but under REA and in RFLP studies, the isolates GVP-063 differed from GVP-072. An opposite case existed with the isolates GVP-072 (biotype 2) and GVP-065 (biotype 5), which appeared similar under REA and in RFLP studies. Finally, reisolates after 8 weeks (GVP-080) from a BV patient (isolates GVP-065) representing the same biotype (biotype 5) differed under REA and in RFLP studies. Thus, lacking any unique DNA fingerprint, G. vaginalis occurring in BV represents a (genetically) mixed population.
Nath
K
K
Long Island University, Department of Biology, Brookville, NY 11548.
Devlin
D
D
Beddoe
A M
AM
D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000145:classification* / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D005260:Female; D006194:Gardnerella vaginalis / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D066298:In Vitro Techniques; D000091402:Prohibitins; D015183:Restriction Mapping; D016585:Vaginosis, Bacterial / Q000145:classification* / Q000235:genetics / Q000382:microbiology
D004269:DNA, Bacterial; C000717331:PHB2 protein, human; D000091402:Prohibitins