Modification of blood group A antigen expression in a pancreatic cancer cell line (PC-1) by inhibitors of N-glycan processing.
Pancreatic adenocarcinomas induced in Syrian hamsters by treatment with N-nitrosobis(2-oxopropyl) amine express blood group A antigen, which is absent in normal pancreatic cells. On membrane glycoproteins purified from tumors, blood group A antigen has been found to be expressed on multiantennary Asn-linked complex glycans. In this study, we investigated the effect of inhibitors of Asn-glycan processing on blood group A antigen bearing glycan structures in a cell line (PC-1) established from a primary induced pancreatic cancer. Expression of blood group A antigen on cells and in membrane preparations was blocked by treatment with 1-deoxymannojirimycin, an inhibitor of mannosidase I, but was retained after treatment with swainsonine, an inhibitor of mannosidase II. However, swainsonine treatment altered the glycan structure associated with blood group A antigen from an endoglycosidase H resistant type to a sensitive type, indicating that the blood group A structure might shift from a complex type to a hybrid type glycan by this treatment. These results demonstrate that Asn-linked glycans carry the major blood group A antigens in PC-1 cells.
Hirota
M
M
Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha 68198-4525.
Mogaki
M
M
Pour
P M
PM
Chaney
W G
WG
D017485:1-Deoxynojirimycin / Q000494:pharmacology*; D000230:Adenocarcinoma / Q000276:immunology*; D000581:Amidohydrolases / Q000378:metabolism; D000818:Animals; D000951:Antigens, Neoplasm / Q000032:analysis / Q000737:chemistry; D001789:Blood Group Antigens / Q000032:analysis* / Q000737:chemistry; D006224:Cricetinae; D004591:Electrophoresis, Polyacrylamide Gel; D005700:Galactosyltransferases / Q000378:metabolism; D005959:Glucosidases / Q000037:antagonists & inhibitors; D006596:Hexosaminidases / Q000378:metabolism; D007150:Immunohistochemistry; D037102:Lectins / Q000378:metabolism; D008647:Mesocricetus; D010190:Pancreatic Neoplasms / Q000276:immunology*; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase; D017026:Swainsonine / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D000951:Antigens, Neoplasm; D001789:Blood Group Antigens; D037102:Lectins; D017485:1-Deoxynojirimycin; D005700:Galactosyltransferases; D005959:Glucosidases; D006596:Hexosaminidases; D000581:Amidohydrolases; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase; D017026:Swainsonine