The wheat mitochondrial genome contains an ORF showing sequence homology to the gene encoding the subunit 6 of the NADH-ubiquinone oxidoreductase.
A region of the mitochondrial (mt) DNA of wheat was studied because of its homology with other plant mtDNAs. Sequence analysis revealed an open reading frame encoding a polypeptide of 247 amino acids. Comparison of the sequence of the putative polypeptide with the protein sequence data of the Swiss-Prot library reveals homology with subunit 6 of the NADH-ubiquinone complex of mitochondria from Marchantia polymorpha, Podospora anserina, Chlamydomonas reinhardtii and of chloroplasts from M. polymorpha and Oryza sativa. No similarity was detected when compared with the subunit 6 of animal mitochondria, probably due to the rapid evolution of the sequence. A single 1.2 kb transcript appears in northern RNA blots. We found 15 edited sites of which only 13 give amino acid changes. This is the first report of a mt nad6 gene in higher plants.
Haouazine
N
N
Laboratoire de Biologie Moléculaire Végétale associé au CNRS (UA 1128), Université Paris Sud, Orsay, France.
Pereira de Souza
A
A
Jubier
M F
MF
Lancelin
D
D
Delcher
E
E
Lejeune
B
B
D000595:Amino Acid Sequence; D001483:Base Sequence; D017343:Genes, Plant / Q000235:genetics*; D016678:Genome; D008928:Mitochondria / Q000502:physiology*; D008969:Molecular Sequence Data; D016660:NAD(P)H Dehydrogenase (Quinone) / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic; D014908:Triticum / Q000201:enzymology / Q000235:genetics*
D012333:RNA, Messenger; D016660:NAD(P)H Dehydrogenase (Quinone)