Innovative endpoint determination system for antifungal susceptibility testing of yeasts.
Fungal infections and antifungal resistance are increasingly recognized. Antifungal susceptibility testing remains unstandardized, and a particularly important problem is endpoint determination. In this paper we propose the yeast metabolic reduction of the tetrazolium salt 2,3-bis(2- methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino)carbonyl]-2H-tetraz olium hydroxide (XTT) as a colorimetric endpoint which is quantitative and objective. Amphotericin B, fluorocytosine, and fluconazole dose-response curves were obtained, and a metabolic MIC could be determined by using precise criteria.
Tellier
R
R
Department of Microbiology, Toronto Hospital, Ontario, Canada.
Krajden
M
M
Grigoriew
G A
GA
Campbell
I
I
D000935:Antifungal Agents / Q000494:pharmacology*; D002175:Candida / Q000187:drug effects; D002176:Candida albicans / Q000187:drug effects; D003124:Colorimetry; D003470:Culture Media; D007202:Indicators and Reagents; D008826:Microbial Sensitivity Tests / Q000592:standards*; D010084:Oxidation-Reduction; D013778:Tetrazolium Salts; D015003:Yeasts / Q000187:drug effects*
D000935:Antifungal Agents; D003470:Culture Media; D007202:Indicators and Reagents; D013778:Tetrazolium Salts; C059087:2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-((phenylamino)carbonyl)-2H-tetrazolium hydroxide