Isolation and characterization of riboflavin carrier protein from human amniotic fluid.
A specific protein exhibiting immunological cross-reactivity with chicken riboflavin carrier protein has been purified to homogeneity from human amniotic fluid by use of ion-exchange and affinity chromatography. The protein is similar to its avian counterpart in terms of molecular size, distribution of 125I-labelled tryptic peptides during finger printing, and preferential binding to riboflavin. Immunologically, they are homologous since most of the monoclonal antibodies raised against the avian protein cross-react with the purified human vitamin carrier.
Prasad
P D
PD
Centre for Reproductive Biology & Molecular Endocrinology, Indian Institute of Science, Bangalore.
Malhotra
P
P
Karande
A A
AA
Adiga
P R
PR
D000653:Amniotic Fluid / Q000737:chemistry*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002352:Carrier Proteins / Q000276:immunology / Q000302:isolation & purification*; D002645:Chickens / Q000276:immunology; D002846:Chromatography, Affinity; D002852:Chromatography, Ion Exchange; D003429:Cross Reactions; D005260:Female; D006801:Humans; D026901:Membrane Transport Proteins; D010449:Peptide Mapping; D011247:Pregnancy; D012256:Riboflavin / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D002352:Carrier Proteins; D026901:Membrane Transport Proteins; C019079:riboflavin-binding protein; D012256:Riboflavin