title: Determination of dextropropoxyphene and norpropoxyphene in plasma by high-performance liquid chromatography.
abstract: A simple and sensitive procedure for the routine assay of the analgesic drug dextropropoxyphene and its main metabolite, norpropoxyphene, in plasma is described. After liquid-liquid extraction from alkalinized plasma and back-extraction into a small volume of an acidic aqueous phase, the aqueous phase was injected into a column packed with 3-microns octadecylsilica particles. Ultraviolet absorbance detection at 210 nm was used. Concentrations down to 2 nM could be determined for both compounds; at this level, the intra-assay coefficient of variation was 5%.
authors.affiliation: Department of Bioanalysis, Astra Arcus AB, Södertälje, Sweden.
authors.forename: K J
authors.initials: KJ
authors.lastname: Pettersson
authors.forename: L B
authors.initials: LB
authors.lastname: Nilsson
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D011431:Dextropropoxyphene / Q000031:analogs & derivatives* / Q000097:blood*; D006801:Humans; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet
chemical_list: C002756:norpropoxyphene; D011431:Dextropropoxyphene