Genetic recombination in brome mosaic virus: effect of sequence and replication of RNA on accumulation of recombinants.
In order to facilitate the isolation of recombinants in brome mosaic virus, a series of duplication mutants with alterations in the RNA3 3' noncoding region has been engineered. The distribution of crossovers, which was observed to be dependent on the parental RNA3 sequence, supported the role of RNA structure in recombination. However, a negative correlation between replication of the parental RNA3 constructs and the accumulation of recombinant progeny confirmed the role of selection.
Nagy
P D
PD
Plant Molecular Biology Center, Northern Illinois University, De Kalb 60115-2861.
Bujarski
J J
JJ
D001483:Base Sequence; D002874:Chromosome Mapping; D003434:Crossing Over, Genetic; D008969:Molecular Sequence Data; D009029:Mosaic Viruses / Q000235:genetics* / Q000472:pathogenicity; D005810:Multigene Family / Q000235:genetics; D016296:Mutagenesis; D010935:Plant Diseases / Q000382:microbiology; D012367:RNA, Viral / Q000378:metabolism*; D011995:Recombination, Genetic; D014774:Virulence; D014779:Virus Replication / Q000235:genetics
D012367:RNA, Viral