Meiotic chromosome condensation and pairing in Saccharomyces cerevisiae studied by chromosome painting.
Non-isotopic high resolution in situ hybridization was applied to cytological preparations of sporulating yeast cells. Ribosomal DNA (rDNA) and chromosome V-specific recombinant lambda clones were used to tag individual chromosomes and chromosome subregions. This allowed the study of chromosome behaviour during early meiotic prophase. It was found that chromatin becomes condensed and homologous DNA sequences then appear to become aligned prior to synaptonemal complex formation.
Scherthan
H
H
Department of Cytology and Genetics, University of Vienna, Austria.
Loidl
J
J
Schuster
T
T
Schweizer
D
D
D002871:Chromosome Banding; D002874:Chromosome Mapping; D015825:Chromosomes, Fungal / Q000648:ultrastructure*; D015342:DNA Probes; D004271:DNA, Fungal / Q000235:genetics; D008540:Meiosis / Q000235:genetics; D009693:Nucleic Acid Hybridization; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000648:ultrastructure*
D015342:DNA Probes; D004271:DNA, Fungal