title: A freezing and thawing method of hamster oocytes designed for both the penetration test and chromosome assay of human spermatozoa.
abstract: Superovulated hamster oocytes were cryopreserved and thawed according to our carefully designed procedures. More than 90% (92 +/- 4%) of oocytes survived freezing and thawing. They were proven to be well conserved, showing excellent performance comparable to freshly ovulated oocytes in the human sperm penetration test (proportion of penetrated ova: 94.7% vs. 93.6%) and human sperm chromosome analysis (proportion of metaphasic ova: 81.8% vs. 83.6%). There was no statistically significant difference in the incidences of sperm chromosome aberrations between assays using fresh and frozen-thawed oocytes. In addition, there was no statistically significant increase of aberrations in female pronuclear (hamster) chromosomes. This freezing-thawing method was found to be reliable, yielding viable hamster oocytes of high quality.
authors.affiliation: Department of Biological Sciences, Asahikawa Medical College, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Tateno
authors.forename: Y
authors.initials: Y
authors.lastname: Kamiguchi
authors.forename: K
authors.initials: K
authors.lastname: Mikamo
mesh_terms: D000782:Aneuploidy; D000818:Animals; D002869:Chromosome Aberrations; D002877:Chromosomes, Human / Q000648:ultrastructure*; D006224:Cricetinae; D015925:Cryopreservation / Q000379:methods*; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D007621:Karyotyping; D008297:Male; D008647:Mesocricetus; D009865:Oocytes; D013084:Sperm-Ovum Interactions