title: Determination of active 5-(N,N-hexamethylene)amiloride in pig plasma.
abstract: A simple, cheap and specific quantitative method for the determination of the selective Na+/H+ exchange inhibitor, 5-(N,N-hexamethylene)amiloride, in plasma and aqueous solutions has been developed. The method involves extraction with ethyl acetate, thin-layer chromatography and spectrofluorodensitometry. The compound was separated from several unidentified metabolites in plasma. The detection limit was 6 x 10(-7) M. The calculated metabolic extraction by the liver was 29%, and the plasma half-life was 12.8 min. The free, active concentration of 5-(N,N-hexamethylene)amiloride was 19.4% of the total concentration, as determined by equilibrium dialysis.
authors.affiliation: Institute for Experimental Medical Research, Ullevaal University Hospital, Oslo, Norway.
authors.forename: M R
authors.initials: MR
authors.lastname: Holthe
authors.forename: O
authors.initials: O
authors.lastname: Brørs
authors.forename: E J
authors.initials: EJ
authors.lastname: Cragoe
authors.forename: T
authors.initials: T
authors.lastname: Veel
authors.forename: M
authors.initials: M
authors.lastname: Raeder
mesh_terms: D000584:Amiloride / Q000031:analogs & derivatives* / Q000097:blood; D000818:Animals; D002855:Chromatography, Thin Layer / Q000379:methods*; D003720:Densitometry / Q000379:methods*; D013552:Swine
chemical_list: C057360:5-(N,N-hexamethylene)amiloride; D000584:Amiloride