Effects of corticosteroids on lymphocyte subpopulations and lymphokine secretion in chickens.
Various effects of glucocorticosteroids on the avian immune system were examined in chickens treated intramuscularly with 0.1 to 2.5 mg dexamethasone or prednisolone. Kinetic changes in body weight gain, percentages of lymphocyte subpopulations, and T-cell functions were examined following treatment with dexamethasone or prednisolone every other day. Chickens treated with dexamethasone or prednisolone showed a decrease in body-weight gain compared with age-matched, untreated chickens. In general, the total number of splenic lymphocytes of chickens treated with the two drugs was significantly lower than in controls in a dose-dependent manner. Flow cytometric analysis of splenic lymphocyte subpopulations revealed that the percentages of lymphocytes expressing CD8, gamma delta T-cell receptor, Ia, or IgM antigens and natural killer cells were lower in dexamethasone-treated chickens than in the controls, whereas the percentages of T lymphocytes bearing CD3, CD4, or alpha beta TCR antigens were higher. Furthermore, splenic T cells obtained from dexamethasone-treated chickens showed a significant depression in concanavalin A-induced lymphoproliferation and interleukin 2 and gamma-interferon production. The results characterize a variety of immunosuppressive effects of glucocorticoids on the avian immune system.
Isobe
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U.S. Department of Agriculture, Beltsville Agricultural Research Center, Maryland 20705.
Lillehoj
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D000818:Animals; D001835:Body Weight / Q000187:drug effects; D002645:Chickens / Q000276:immunology*; D003208:Concanavalin A; D003907:Dexamethasone / Q000494:pharmacology*; D007371:Interferon-gamma / Q000187:drug effects / Q000378:metabolism; D007376:Interleukin-2 / Q000378:metabolism; D008213:Lymphocyte Activation / Q000187:drug effects; D016131:Lymphocyte Subsets / Q000187:drug effects; D008214:Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D008222:Lymphokines / Q000187:drug effects / Q000378:metabolism*; D011239:Prednisolone / Q000494:pharmacology*
D007376:Interleukin-2; D008222:Lymphokines; D003208:Concanavalin A; D003907:Dexamethasone; D007371:Interferon-gamma; D011239:Prednisolone