A microwell sperm penetration assay.
Egg penetration rates in a modified SPA using microwells in tissue typing plates were comparable with those in a standard assay. This technique allows sperm penetrating ability to be determined using single zona-free hamster eggs and as few as 10,000 spermatozoa.
Bronson
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Department of Obstetrics and Gynecology, State University of New York, Stony Brook 11794-8091.
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D000818:Animals; D006224:Cricetinae; D005260:Female; D006801:Humans; D008297:Male; D008647:Mesocricetus; D013081:Sperm Motility; D013084:Sperm-Ovum Interactions; D013094:Spermatozoa / Q000502:physiology