High expression of manganese superoxide dismutase in 7,12-dimethylbenz[a]anthracene-induced ovarian cancer and increased serum levels in the tumor-bearing rats.
Manganese superoxide dismutase (Mn-SOD) is strongly expressed in human ovarian cancer, and the serum level of the enzyme is a useful marker for the diagnosis and monitoring of human ovarian cancer. In the present study we found that Mn-SOD was highly expressed in primary and transplanted ovarian cancers in rats induced by 7,12-dimethylbenz[a]anthracene (DMBA), as judged by enzyme-linked immunosorbent assay as well as by Northern blot analysis. The serum levels of Mn-SOD in the tumor-bearing rats were also higher than those in control rats. The antibody strongly reacted with rat ovarian carcinoma tissues. These data suggest that DMBA-induced ovarian cancer in rats is a good experimental model for human ovarian cancer, and that Mn-SOD is also a good marker for disease in the animal model.
Nakata
T
T
Department of Biochemistry, Osaka University Medical School, Suita, Japan.
Suzuki
K
K
Fujii
J
J
Ishikawa
M
M
Tatsumi
H
H
Sugiyama
T
T
Nishida
T
T
Shimizu
T
T
Yakushiji
M
M
Taniguchi
N
N
D015127:9,10-Dimethyl-1,2-benzanthracene; D000230:Adenocarcinoma / Q000139:chemically induced / Q000201:enzymology*; D000818:Animals; D005260:Female; D007150:Immunohistochemistry; D009368:Neoplasm Transplantation; D010051:Ovarian Neoplasms / Q000139:chemically induced / Q000201:enzymology*; D012334:RNA, Neoplasm / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000097:blood / Q000378:metabolism*
D012334:RNA, Neoplasm; D015127:9,10-Dimethyl-1,2-benzanthracene; D013482:Superoxide Dismutase