Column liquid chromatography and microbiological assay compared for determination of cefadroxil preparations.
A reversed-phase column liquid chromatographic method was developed for the assay of cefadroxil in bulk drugs and pharmaceutical preparations. An equation was derived showing a linear relationship between peak-area ratios of cefadroxil to dimethylphthalate (internal standard) and the cefadroxil concentration over a range of 0.02-0.8 mg/ml (r = 0.9999). Standard addition recoveries were generally greater than 97.7%. The coefficients of variation in the within-day assay were between 0.36 and 0.65, and in the between-day assay was 0.71%. The column liquid chromatographic assay results were compared with those obtained from a microbiological assay, which indicated that the proposed method is a suitable substitute for the microbiological method for potency assays and stability studies of cefadroxil preparations.
Hsu
M C
MC
National Laboratories of Foods and Drugs, Department of Health, Taipei, Taiwan.
Chang
Y W
YW
Lee
Y T
YT
D001412:Bacillus subtilis / Q000187:drug effects; D002434:Cefadroxil / Q000032:analysis* / Q000494:pharmacology / Q000592:standards; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000706:statistics & numerical data; D004355:Drug Stability; D011786:Quality Control
D002434:Cefadroxil