Model invariant method for extracting single-channel mean open and closed times from heterogeneous multichannel records.
We present a proof that the mean open (and closed) times of the individual channels in a multichannel record can be found in a model-independent fashion. As the results are model independent, they can be derived by assuming the simplest model for all the channels, namely that they all have the basic CLOSED in equilibrium with OPEN scheme. In particular, the method can be applied to patches where the channel population is heterogenous with respect to open probability. Multichannel simulations are performed to test the limits of applicability of this method to restricted amounts of data. One conclusion is that increasing the number of channels does not substantially reduce the errors in estimating the mean times, in spite of the 'increased information' present. We also prove the general applicability of the algorithm of Fenwick et al. (1982) in estimating the mean times without knowledge of the number of channels present, and discuss its limitations. An illustration using experimental data is also given.
Ramanan
S V
SV
Department of Physiology and Biophysics, State University of New York, Stony Brook 11794-8661.
Fan
S F
SF
Brink
P R
PR
D000465:Algorithms; D004594:Electrophysiology / Q000295:instrumentation*; D007700:Kinetics; D008954:Models, Biological