Effects of chloroquine on the photosensory membrane turnover and the ultrastructure of lysosome-related bodies of the crayfish photoreceptor.
The effect of chloroquine in combination with bright light on the ultrastructure of crayfish (Orconectes limosus R.) photoreceptors was investigated in vivo. Chloroquine had several effects upon the crayfish retina. Multivesicular bodies (MVB) that are involved in lysosomal degradation of photosensory membrane were altered in ultrastructure. MVB frequently contained smaller MVB in a state of more advanced membrane degradation. Additionally screening pigment-like granules appeared in MVB. MVB accumulated in and filled the retinular cell cytoplasm just proximal or distal to the basement membrane which indicated inhibition of photosensory membrane degradation. Under chloroquine treatment rhabdom degradation appeared to be inhibited, as rhabdom diameter was less reduced under these conditions. Also chloroquine caused accumulation of screening pigment granules in glial cells within the lamina ganglionaris.
Schraermeyer
U
U
Institut für Biologie II (Zoologie), RWTH Aachen, Bundesrepublik Deutschland.
D000818:Animals; D003400:Astacoidea; D001485:Basement Membrane / Q000187:drug effects / Q000648:ultrastructure; D002738:Chloroquine / Q000494:pharmacology*; D003594:Cytoplasmic Granules / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D007425:Intracellular Membranes / Q000187:drug effects / Q000502:physiology / Q000648:ultrastructure; D008027:Light; D008247:Lysosomes / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D008854:Microscopy, Electron; D015388:Organelles / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D010786:Photoreceptor Cells / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure
D002738:Chloroquine