title: Identification of the KDR tyrosine kinase as a receptor for vascular endothelial cell growth factor.
abstract: Vascular endothelial cell growth factor (VEGF), also known as vascular permeability factor, is an endothelial cell mitogen which stimulates angiogenesis. Here we report that a previously identified receptor tyrosine kinase gene, KDR, encodes a receptor for VEGF. Expression of KDR in CMT-3 (cells which do not contain receptors for VEGF) allows for saturable 125I-VEGF binding with high affinity (KD = 75 pM). Affinity cross-linking of 125I-VEGF to KDR-transfected CMT-3 cells results in specific labeling of two proteins of M(r) = 195 and 235 kDa. The KDR receptor tyrosine kinase shares structural similarities with a recently reported receptor for VEGF, flt, in a manner reminiscent of the similarities between the alpha and beta forms of the PDGF receptors.
authors.affiliation: Medical Research Division, Lederle Laboratories, Pearl River, New York 10965.
authors.forename: B I
authors.initials: BI
authors.lastname: Terman
authors.forename: M
authors.initials: M
authors.lastname: Dougher-Vermazen
authors.forename: M E
authors.initials: ME
authors.lastname: Carrion
authors.forename: D
authors.initials: D
authors.lastname: Dimitrov
authors.forename: D C
authors.initials: DC
authors.lastname: Armellino
authors.forename: D
authors.initials: D
authors.lastname: Gospodarowicz
authors.forename: P
authors.initials: P
authors.lastname: Böhlen
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D006801:Humans; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D011505:Protein-Tyrosine Kinases / Q000235:genetics*; D011975:Receptors, Mitogen / Q000235:genetics*; D040262:Receptors, Vascular Endothelial Growth Factor; D016415:Sequence Alignment
chemical_list: D009838:Oligodeoxyribonucleotides; D011975:Receptors, Mitogen; D011505:Protein-Tyrosine Kinases; D040262:Receptors, Vascular Endothelial Growth Factor