title: Primary structure of a cardioactive neuropeptide from the tobacco hawkmoth, Manduca sexta.
abstract: The amino acid sequence of the first of a family of insect cardioregulatory peptides from the tobacco hawkmoth, Manduca sexta, has been determined using a combination of Edman degradation microsequencing and mass spectroscopy. This peptide contains 9 amino acid residues and an observed mass for the monoisotopic protonated molecule of 956.4 Da. There are two cysteines at positions 3 and 9 forming a disulfide bridge and the carboxyl-terminus is amidated. The structure of this peptide, Pro-Phe-Cys-Asn-Ala-Phe-Thr-Gly-Cys-NH2, is identical to a peptide recently isolated from crabs called crustacean cardioactive peptide (CCAP) and we propose that this peptide be named Manduca CCAP.
authors.affiliation: Institute of Neuroscience, University of Oregon, Eugene 97403.
authors.forename: C C
authors.initials: CC
authors.lastname: Cheung
authors.forename: P K
authors.initials: PK
authors.lastname: Loi
authors.forename: A W
authors.initials: AW
authors.lastname: Sylwester
authors.forename: T D
authors.initials: TD
authors.lastname: Lee
authors.forename: N J
authors.initials: NJ
authors.lastname: Tublitz
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D007301:Insect Hormones / Q000737:chemistry*; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D009036:Moths / Q000737:chemistry*; D009842:Oligopeptides / Q000737:chemistry*; D011761:Pyrrolidonecarboxylic Acid / Q000031:analogs & derivatives
chemical_list: D007301:Insect Hormones; D009842:Oligopeptides; C044833:adipokinetic hormone; D011761:Pyrrolidonecarboxylic Acid