[Advances in automated amino acid analysis].
Recent progress in the field of amino-acid analysis, characterized by the development of fully automated chromatographic equipment, is described. For separation and quantitation of free amino acids from biological fluid, such as blood and urine, ion exchange column chromatography followed by colorimetric detection of ninhydrin reactive products is the most widely adopted methodology in the clinical field. Analytic procedures are fully automated. The computer controlled pumping mechanism guarantees a precise buffer supply for separation and peak identification and peak height or/and area detection of each amino acid in the effluent are highly reproducible. Complete analysis of more than 30 amino acids in biological fluids can be performed within 2 hours. Amino acid analysis is now easily adopted in the routine clinical laboratory and is expected to contribute to the clinical diagnosis of amino acidopathies.
Endo
J
J
Dept. of Laboratory Medicine, Shimane Medical University.
Notsu
Y
Y
D000596:Amino Acids / Q000032:analysis* / Q000097:blood / Q000652:urine; D002852:Chromatography, Ion Exchange / Q000295:instrumentation / Q000379:methods*; D006801:Humans
D000596:Amino Acids