title: Atypical pyramidal cells in epileptic human cortex: CFLS and 3-D reconstructions.
abstract: Epileptic temporal cortices, removed from 3 patients with intractable partial epilepsy (IPE) during neurosurgery, were studied. Pyramidal neurons (40-50 per slice) in laminae III, V and white matter, were injected with lucifer yellow. Samples were examined in a confocal laser scanning microscope (Biorad 600) and individual cells scanned at 0.1-1 microns incremental levels. 2-D maximal linear projection was used for overview. Frames (50-60) of scanned neurons were transformed into 3-D volumes, using VoxelView software on a Silicone Graphics workstation and rotated. All samples contained neurons with duplicated apical dendrites, additional basal dendrites or were misplaced in a horizontal position in the white matter. The relation between these preliminary observations and the disease is discussed.
authors.affiliation: Brain Research Institute, Academy of Sciences, Russia.
authors.forename: P
authors.initials: P
authors.lastname: Belichencko
authors.forename: A
authors.initials: A
authors.lastname: Dahlström
authors.forename: C
authors.initials: C
authors.lastname: von Essen
authors.forename: S
authors.initials: S
authors.lastname: Lindström
authors.forename: C
authors.initials: C
authors.lastname: Nordborg
authors.forename: P
authors.initials: P
authors.lastname: Sourander
mesh_terms: D004828:Epilepsies, Partial / Q000473:pathology*; D005456:Fluorescent Dyes; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007546:Isoquinolines; D007834:Lasers; D009474:Neurons / Q000648:ultrastructure; D013702:Temporal Lobe / Q000473:pathology* / Q000648:ultrastructure
chemical_list: D005456:Fluorescent Dyes; D007546:Isoquinolines; C017475:lucifer yellow