title: Chromosome painting using chromosome-specific probes from flow-sorted pig chromosomes.
abstract: Biotinylated chromosome-specific probes were prepared from flow-sorted pig chromosomes 1, 13, 18, X, and Y using the degenerate oligonucleotide-primed polymerase chain reaction. Probes prepared in this way can be used to confirm the identity of chromosomes in the bivariate pig flow karyotype and in pig x mouse somatic cell hybrids.
authors.affiliation: AFRC Institute of Animal Physiology and Genetics Research, Babraham, Cambridge, UK.
authors.forename: C F
authors.initials: CF
authors.lastname: Langford
authors.forename: H
authors.initials: H
authors.lastname: Telenius
authors.forename: N P
authors.initials: NP
authors.lastname: Carter
authors.forename: N G
authors.initials: NG
authors.lastname: Miller
authors.forename: E M
authors.initials: EM
authors.lastname: Tucker
mesh_terms: D000818:Animals; D001483:Base Sequence; D005434:Flow Cytometry; D006822:Hybrid Cells; D017403:In Situ Hybridization; D007621:Karyotyping / Q000379:methods*; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes / Q000235:genetics*; D016133:Polymerase Chain Reaction; D013552:Swine
chemical_list: D015345:Oligonucleotide Probes