Analysis of glycated albumin in postmortem blood samples as the diagnostic parameters of diabetes mellitus.
Glycated hemoglobin (GHb), fructosamine and glycated albumin (GA) in hemolytic sera from cadavers were analyzed for the postmortem diagnosis of diabetes mellitus. The levels of GHb and fructosamine were determined by boronate affinity chromatography and colorimetry, respectively. Albumin fraction was isolated from the samples by Affi-Gel Blue affinity chromatography. The glycated and non-glycated molecules were separated by boronate affinity chromatography, and quantitated by bromcresol green method. Fructosamine could not be analyzed from highly hemolytic sera containing more than 10 g/l hemoglobin. In such samples, the levels of GHb and GA were deviated from the standard values, indicating their postmortem degradation. In less hemolytic samples, GA was as informative as GHb and fructosamine for the diagnosis of diabetes mellitus.
Akane
A
A
Department of Legal Medicine, Shimane Medical University, Izumo, Japan.
Shiono
H
H
Matsubara
K
K
Tanabe
K
K
Nakamura
H
H
Hasegawa
M
M
Kagawa
M
M
D001800:Blood Specimen Collection; D002846:Chromatography, Affinity; D003920:Diabetes Mellitus / Q000097:blood / Q000175:diagnosis*; D019270:Fructosamine; D006442:Glycated Hemoglobin / Q000032:analysis*; D017127:Glycation End Products, Advanced; D006031:Glycosylation; D006595:Hexosamines / Q000097:blood; D006801:Humans; D011180:Postmortem Changes; D012709:Serum Albumin / Q000032:analysis*; D000093423:Glycated Serum Albumin
D019270:Fructosamine; D006442:Glycated Hemoglobin; D017127:Glycation End Products, Advanced; D006595:Hexosamines; D012709:Serum Albumin; D000093423:Glycated Serum Albumin