title: Alw26I, Eco31I and Esp3I--type IIs methyltransferases modifying cytosine and adenine in complementary strands of the target DNA.
abstract: The specificity of three DNA methyltransferases M.Alw26I, M.Eco31I and M.Esp3I, isolated from Acinetobacter Iwoffi RFL26, Escherichia coli RFL31 and Hafnia alvei RFL3+, respectively, was determined. All the enzymes methylate both strands of asymmetric recognition sites yielding m5C in the top-strand and m6A in the bottom-strand, as below: 5'-GTm5CTC 5'-GGTm5CTC 5'-CGTm5CTC 3'-Cm6AGAG 3'-CCm6AGAG 3'-GCm6AGAG (M.Alw26I) (M.Eco31I) (M.Esp3I) They are the first members of type IIs methyltransferases that modify different types of nucleotides in the recognition sequence.
authors.affiliation: Institute of Biotechnology FERMENTAS, Vilnius, Lithuania.
authors.forename: J
authors.initials: J
authors.lastname: Bitinaite
authors.forename: Z
authors.initials: Z
authors.lastname: Maneliene
authors.forename: S
authors.initials: S
authors.lastname: Menkevicius
authors.forename: S
authors.initials: S
authors.lastname: Klimasauskas
authors.forename: V
authors.initials: V
authors.lastname: Butkus
authors.forename: A
authors.initials: A
authors.lastname: Janulaitis
mesh_terms: D000150:Acinetobacter / Q000201:enzymology*; D001483:Base Sequence; D015254:DNA Modification Methylases / Q000378:metabolism*; D015257:DNA-Cytosine Methylases / Q000378:metabolism*; D004755:Enterobacteriaceae / Q000201:enzymology*; D004926:Escherichia coli / Q000201:enzymology*; D007700:Kinetics; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000378:metabolism; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific) / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: D009838:Oligodeoxyribonucleotides; D015254:DNA Modification Methylases; C077513:DNA modification methylase Alw26I; C077514:DNA modification methylase Eco31I; C077515:DNA modification methylase Esp3I; D015257:DNA-Cytosine Methylases; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific)