Detection of a polymorphism within the pepsinogen C gene with PCR: construction of a linkage map around PGC from 6p11-6p21.3.
An insertion/deletion polymorphism between exons 7 and 8 of the pepsinogen C gene (PGC), previously detectable with Southern analysis, was formatted for detection with PCR. Alleles were rapidly typed by UV irradiation of ethidium bromide-stained agarose gels. Whereas Southern analysis revealed two alleles, the smaller fragments generated with PCR allowed the resolution of three alleles that were previously scored as a single allele and increased the heterozygosity of the system from 0.20 to 0.53. After a set of reference families was genotyped with the PCR-based polymorphism, a linkage map around the PGC gene on chromosome 6 was constructed. This included the HLA cluster and the highly informative D6S223 locus. PGC lies 22 cM proximal to HLA-DPB and between D6S5 and D6S4 at distances of 4.5 and 13.1 cM, respectively.
Bowcock
A M
AM
Department of Pediatrics, University of Texas Southwestern Medical Center, Dallas 75235.
Azuma
T
T
Barnes
R I
RI
Wu
S H
SH
Bell
G I
GI
Taggart
R T
RT
D001483:Base Sequence; D015139:Blotting, Southern; D002874:Chromosome Mapping; D002896:Chromosomes, Human, Pair 6; D004277:DNA, Single-Stranded; D005260:Female; D008040:Genetic Linkage; D006579:Heterozygote; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D010375:Pedigree; D010435:Pepsinogens / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic
D004277:DNA, Single-Stranded; D010435:Pepsinogens