Secretion of bovine alpha-lactalbumin into the milk of transgenic rats.
Bovine alpha-lactalbumin (b alpha LA) gene prepared by polymerase chain reaction was introduced into rat genomes by microinjection. Out of 17 transgenic rat lines, 11 secreted b alpha LA into their milk at concentrations higher than 0.2 micrograms/ml. Of these, three lines secreted b alpha LA at concentrations higher than those in bovine milk. The highest concentration of b alpha LA secreted into rat milk was 2,400 micrograms/ml.
Hochi
S
S
Research Institute of Life Science, Snow Brand Milk Products, Co., Ltd., Tochigi, Japan.
Ninomiya
T
T
Waga-Homma
M
M
Sagara
J
J
Yuki
A
A
D000818:Animals; D030801:Animals, Genetically Modified / Q000502:physiology*; D001483:Base Sequence; D002417:Cattle / Q000235:genetics*; D005260:Female; D007768:Lactalbumin / Q000378:metabolism*; D007774:Lactation; D008845:Microinjections; D008892:Milk / Q000737:chemistry*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D051381:Rats / Q000502:physiology*; D013678:Technology, Pharmaceutical / Q000379:methods*
D007768:Lactalbumin