title: Protein A fusion vectors for use in combination with pEX vectors in the production and affinity purification of specific antibodies.
abstract: New vectors expressing protein A fusions were constructed, based upon the kanamycin-resistance-encoding plasmid, pK19. They were designed for use in combination with the pEX series of beta-galactosidase protein-fusion vectors in the production and affinity purification of specific antibodies.
authors.affiliation: Department of Biochemistry, University of Edinburgh Medical School, Scotland, UK.
authors.forename: J
authors.initials: J
authors.lastname: Zueco
authors.forename: A
authors.initials: A
authors.lastname: Boyd
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000908:Antibodies, Fungal / Q000096:biosynthesis / Q000276:immunology / Q000302:isolation & purification; D001483:Base Sequence; D004274:DNA, Recombinant; D005656:Fungal Proteins / Q000235:genetics / Q000276:immunology; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D010957:Plasmids; D011817:Rabbits; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology; D013205:Staphylococcal Protein A / Q000235:genetics*
chemical_list: D000908:Antibodies, Fungal; D004274:DNA, Recombinant; D005656:Fungal Proteins; D011993:Recombinant Fusion Proteins; D013205:Staphylococcal Protein A