Periodic acid/Schiff staining of glycoproteins immobilized on a blotting matrix.
A simple and sensitive method for the detection of glycoproteins and glycopeptides in solution and in polyacrylamide gels is described. This method combines the well-known periodic acid/Schiff stain with protein blotting. Compared with direct staining of a polyacrylamide gel, the sensitivity is considerably increased. Using a set of glycoproteins, we have found the sensitivity to be about 4 ng carbohydrate.
Strömqvist
M
M
Department of Protein Chemistry, Symbicom AB, UMEA, Sweden.
Gruffman
H
H
D000818:Animals; D002417:Cattle; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins / Q000032:analysis*; D015151:Immunoblotting; D010503:Periodic Acid-Schiff Reaction; D011817:Rabbits; D012680:Sensitivity and Specificity
D006023:Glycoproteins