Neuronal survival and neurite extension supported by astrocytes co-cultured in transwells.
The influence of astrocytes on the development of cerebral cortical neurons was studied in a co-culture system using transwells with chemically defined medium. Cerebral cortical neurons from 15- or 16-day-old mouse embryo were cultured in the lower wells which were separated by a porous membrane from the upper transwells where cerebral cortical astrocytes from newborn mouse were cultured. Neurons co-cultured with astrocytes for 7 days formed a network-like web and maintained a slightly better survival from 4 to 7 days. However, neurons cultured in conditioned medium obtained from astrocytes did not form any network after 7 days even though they maintained a better cell survival at 4 days.
Walsh
E
E
New Drug Research Laboratories, Fujisawa Pharmaceutical Co., Ltd., Osaka, Japan.
Ueda
Y
Y
Nakanishi
H
H
Yoshida
K
K
D000818:Animals; D001253:Astrocytes / Q000166:cytology / Q000502:physiology*; D002450:Cell Communication; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology / Q000502:physiology; D046508:Culture Techniques / Q000295:instrumentation; D004622:Embryo, Mammalian; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D051379:Mice; D008815:Mice, Inbred Strains; D016501:Neurites / Q000502:physiology / Q000648:ultrastructure*; D009474:Neurons / Q000166:cytology* / Q000502:physiology / Q000648:ultrastructure
D005904:Glial Fibrillary Acidic Protein