Studies of the interaction of the maltose-binding protein of Escherichia coli, a closed-groove binder, with 4,6-O-ethylidenemalto-oligosaccharides (dp 2-5) and its regioselective labelling with 3-azibutyl 1-thio-alpha-(6-3H)maltoside.
Four malto-oligosaccharides (dp 2-5), each with a 4,6-O-ethylidene group on the glucosyl unit at the non-reducing terminus, were synthesised and used to prove that the maltose-binding protein (MBP) of E. coli is a closed-groove binder. alpha-D-Glucosylation of 3-azibutyl 1-thio-alpha-D-(6-3H)glucopyranoside yielded a 3H-labelled, photolabile 1-thiomaltoside derivative that was used to chemically modify the binding site of MBP. The 3H-labelled peptide containing 83% of the total radioactivity, which was isolated after tryptic cleavage of the modified MBP and sequenced, is part of the closed end of the MBP groove.
Lehmann
J
J
Institut für Organische Chemie und Biochemie der Universität Freiburg, Germany.
Schiltz
E
E
Steck
J
J
D018528:ATP-Binding Cassette Transporters; D000595:Amino Acid Sequence; D002240:Carbohydrate Sequence; D002352:Carrier Proteins / Q000737:chemistry*; D004926:Escherichia coli / Q000737:chemistry*; D029968:Escherichia coli Proteins; D008320:Maltose / Q000737:chemistry*; D058619:Maltose-Binding Proteins; D008757:Methylglucosides / Q000737:chemistry*; D008969:Molecular Sequence Data; D009004:Monosaccharide Transport Proteins; D009844:Oligosaccharides / Q000737:chemistry; D011487:Protein Conformation
D018528:ATP-Binding Cassette Transporters; D002352:Carrier Proteins; D029968:Escherichia coli Proteins; D058619:Maltose-Binding Proteins; D008757:Methylglucosides; D009004:Monosaccharide Transport Proteins; D009844:Oligosaccharides; C461599:maltose transport system, E coli; D008320:Maltose