Sn-chlorin e6 antibacterial immunoconjugates. An in vitro and in vivo analysis.
Monoclonal antibody-Sn-chlorin e6 immunoconjugates were prepared by the site-selective covalent modification of the monoclonal oligosaccharide moiety. By carefully controlling the reaction conditions and introducing triethanolamine groups as axial ligands of the Sn moiety, conjugates with in vivo biodistribution properties similar to underivatized IgG were prepared. By varying the reaction conditions, conjugates were reproducibly prepared with a range of photosensitizer to mAb molar ratios from 1.6 to 10. Based on a competitive inhibition radioimmunoassay, conjugates prepared by this method showed selectivity and binding affinity comparable to the unmodified antibody. The immunoconjugates had only slightly lower singlet oxygen yields than that observed with the Sn-chlorin e6 precursor indicating that negligible aggregation or structural modification of the chromophores occurred during the synthesis process. In vitro cell killing experiments demonstrated that all conjugates possessed significant cytotoxic activity. Biodistribution studies in mice showed that conjugates prepared with axial ligands had significant serum retention 24 h after injection while conjugates prepared without the triethanolamine ligand were much more rapidly cleared. In vivo specificity was demonstrated using rats infected with Fisher immunotype I P. aeruginosa at a site in the left posterior thigh muscle. Target to background ratios exceeded 60 at 120 h after conjugate injection of the specific immunoconjugate, compared to a ratio of only 6 for a non-specific mouse IgG conjugate. Biodistribution patterns at 120 h post injection indicate that the conjugates were both biologically active and structurally intact.
Lu
X M
XM
Surgical Services, Massachusetts General Hospital, Boston 02114.
Fischman
A J
AJ
Stevens
E
E
Lee
T T
TT
Strong
L
L
Tompkins
R G
RG
Yarmush
M L
ML
D000818:Animals; D000907:Antibodies, Bacterial / Q000737:chemistry / Q000378:metabolism; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000378:metabolism; D000936:Antigen-Antibody Complex / Q000378:metabolism; D002735:Chlorophyllides; D003602:Cytotoxicity, Immunologic; D004983:Ethanolamines / Q000737:chemistry; D006023:Glycoproteins / Q000737:chemistry; D007074:Immunoglobulin G / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D051379:Mice; D010777:Photochemistry; D011166:Porphyrins / Q000737:chemistry*; D011550:Pseudomonas aeruginosa / Q000276:immunology; D011863:Radioimmunoassay; D051381:Rats; D014001:Tin
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D002735:Chlorophyllides; D004983:Ethanolamines; D006023:Glycoproteins; D007074:Immunoglobulin G; D011166:Porphyrins; C062985:phytochlorin; D014001:Tin; C009546:triethanolamine