The degree of bacterial translocation is a determinant factor for mortality after burn injury and is improved by prostaglandin analogs.
Bacterial translocation and related mortality rates were examined in previously transfused BALB/c mice that were gavaged with 14C radioisotope-labeled Escherichia coli before inflicting a 20% full-thickness flame burn. Radionuclide counts were measured in blood obtained by retro-orbital puncture 4 hours postburn, and survival was recorded for 10 days. Radionuclide counts in the blood correlated well with both radionuclide counts and numbers of viable bacterial in the tissues. Survivors had significantly less bacterial translocation as evidenced by blood radionuclide counts compared with nonsurvivors, and there was a significant inverse correlation between the degree of translocation and the length of survival. In the next experiment, the prostaglandin E (PGE) analogs misoprostol, enisoprost, or 16,16-dimethyl PGE2 were administered to transfused animals for 3 days before burn. Prostaglandin E analogs significantly reduced bacterial translocation as measured by blood radionuclide counts 4 hours postburn and improved survival. The data demonstrate that the intensity of bacterial translocation after burn injury is significantly associated with subsequent death. Improvement of survival by PGE analogs is associated with decreased bacterial translocation.
Fukushima
R
R
Department of Surgery, University of Cincinnati Medical Center 45267-0558.
Gianotti
L
L
Alexander
J W
JW
Pyles
T
T
D015064:16,16-Dimethylprostaglandin E2 / Q000494:pharmacology; D000527:Alprostadil / Q000031:analogs & derivatives / Q000494:pharmacology; D000818:Animals; D002056:Burns / Q000188:drug therapy / Q000382:microbiology* / Q000401:mortality; D015169:Colony Count, Microbial; D004926:Escherichia coli / Q000254:growth & development*; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D016595:Misoprostol / Q000494:pharmacology; D011459:Prostaglandins E, Synthetic / Q000494:pharmacology*
D011459:Prostaglandins E, Synthetic; D016595:Misoprostol; D000527:Alprostadil; C054594:enisoprost; D015064:16,16-Dimethylprostaglandin E2