Inhibition of morphine dependence by a lipopolysaccharide from Pantoea agglomerans.
A lipopolysaccharide from Pantoea agglomerans (LPSp) was purified and examined for relief of morphine dependence by observing its inhibition of the jumping of mice on naloxone-precipitate withdrawal. Administration of LPSp either intravenously or intradermally showed marked inhibition of the jumping. Beta-endorphin in mouse serum and brain tissue were recognized to be in synchrony with the time course of the relief. Administration of TNF-alpha gave similar effect, suggesting that LPSp induces a cytokine cascade to produce endogenous TNF followed by ACTH/beta-LPH gene products and beta-endorphin. The effect of LPSp was better than that of LPS from E. coli or Bordetella pertussis, and thus is considered to be applicable for clinical use.
Okutomi
T
T
Biotechnology Research Center, Teikyo University, Kawasaki, Japan.
Nishizawa
T
T
Inagawa
H
H
Soma
G
G
Minami
M
M
Satoh
M
M
Mizuno
D
D
D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D001921:Brain / Q000378:metabolism; D016207:Cytokines / Q000096:biosynthesis; D004885:Erwinia; D008070:Lipopolysaccharides / Q000302:isolation & purification / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D009021:Morphine Dependence / Q000188:drug therapy*; D009270:Naloxone; D013375:Substance Withdrawal Syndrome / Q000188:drug therapy / Q000209:etiology / Q000523:psychology; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis / Q000494:pharmacology; D001615:beta-Endorphin / Q000096:biosynthesis / Q000097:blood
D016207:Cytokines; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; C524847:lipopolysaccharide IP-PA1, Pantoea agglomerans; D009270:Naloxone; D001615:beta-Endorphin