title: 2,4-Dichlorophenoxyacetic acid action on in vitro protein synthesis and its relation to polyamines.
abstract: The effect of 2,4-dichlorophenoxyacetic acid (2,4-D) on the in vitro synthesis of proteins was studied in Chinese hamster ovary cells. A remarkable inhibition of the synthesis of proteins was observed when cells grew for 24 h in presence of 1 mM 2,4-D. This effect was reversed by adding 0.1 mM of the three polyamines (putrescine, spermidine and spermine) to the cultured cells. The mRNA is not altered, indicating that the 2,4-D action is located at a different locus, which may be the ribosomes. From these studies, one can speculate that the alterations in the protein synthesis may be a consequence of the effect of 2,4-D on the polyamine metabolism.
authors.affiliation: Departamento Biologia Molecular, Facultad de Ciencias Exactas, Fisico-Quimicas y Naturales, Universidad Nacional de Rio Cuarto, Córdoba, Argentina.
authors.forename: V
authors.initials: V
authors.lastname: Rivarola
authors.forename: G
authors.initials: G
authors.lastname: Mori
authors.forename: H
authors.initials: H
authors.lastname: Balegno
mesh_terms: D015084:2,4-Dichlorophenoxyacetic Acid / Q000494:pharmacology*; D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D016466:CHO Cells / Q000187:drug effects / Q000378:metabolism; D006224:Cricetinae; D011073:Polyamines / Q000378:metabolism*; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D000596:Amino Acids; D011073:Polyamines; D012333:RNA, Messenger; D015084:2,4-Dichlorophenoxyacetic Acid