A vasopressor factor partially purified from human parathyroid glands.
Recently, a parathyroid hypertensive factor was postulated to play a role in the pathogenesis of hypertension in genetically hypertensive rats. Therefore it was examined, whether in human parathyroid glands a vasopressor substance can be detected. For this purpose, homogenates of hyperplastic parathyroid glands from 20 patients with tertiary hyperparathyroidism were deproteinized and fractionated by gel chromatography. The fractions obtained were tested for vasopressor activity in isolated perfused rat kidneys. A vasopressor fraction containing substances of 0.6-2.5 kDa was identified in the parathyroid glands. The responsible product was heat sensitive, peptidase-, trypsin- and carboxypeptidase y- sensitive and hydrophilic, as it did not bind to hydrophobic reversed-phase gel. These results suggest that parathyroid glands contain a hydrophilic peptide-like vasopressor substance different from the parathyroid hormone.
Schlüter
H
H
Med. Poliklinik, University of Münster, Germany.
Quante
C
C
Buchholz
B
B
Dietl
K H
KH
Spieker
C
C
Karas
M
M
Zidek
W
W
D000804:Angiotensin II / Q000494:pharmacology; D000818:Animals; D010450:Endopeptidases / Q000494:pharmacology; D006801:Humans; D006961:Hyperparathyroidism / Q000209:etiology / Q000601:surgery; D006973:Hypertension / Q000209:etiology; D066298:In Vitro Techniques; D007668:Kidney / Q000187:drug effects / Q000502:physiology*; D007676:Kidney Failure, Chronic / Q000150:complications; D008297:Male; D013058:Mass Spectrometry; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000502:physiology; D010280:Parathyroid Glands / Q000737:chemistry*; D051381:Rats; D011921:Rats, Inbred WKY; D012077:Renal Artery / Q000187:drug effects / Q000502:physiology; D012079:Renal Circulation / Q000187:drug effects*; D014020:Tissue Extracts / Q000494:pharmacology*; D014662:Vasoconstrictor Agents / Q000302:isolation & purification* / Q000494:pharmacology
D014020:Tissue Extracts; D014662:Vasoconstrictor Agents; D000804:Angiotensin II; D010450:Endopeptidases