title: Osmostress-induced changes in yeast gene expression.
abstract: When Saccharomyces cerevisiae cells are exposed to high concentration of NaCl, they show reduced viability, methionine uptake and protein biosynthesis. Cells can acquire tolerance against a severe salt shock (up to 1.4 M NaCl) by a previous treatment with 0.7 M NaCl, but not by a previous heat shock. Two-dimensional analysis of [3H]-leucine-labelled proteins from salt-shocked cells (0.7 M NaCl) revealed the elevated rate of synthesis of nine proteins, among which were the heat-shock proteins hsp12 and hsp26. Northern analysis using gene-specific probes confirmed the identity of the latter proteins and, in addition, demonstrated the induction of glycerol-3-phosphate dehydrogenase gene expression. The synthesis of the same set of proteins is induced or enhanced upon exposure of cells to 0.8 M sucrose, although not as dramatically as in an iso-osmolar NaCl concentration (0.7 M).
authors.affiliation: Department of Biochemistry and Molecular Biology, Vrije Universiteit, Amsterdam, The Netherlands.
authors.forename: J C
authors.initials: JC
authors.lastname: Varela
authors.forename: C
authors.initials: C
authors.lastname: van Beekvelt
authors.forename: R J
authors.initials: RJ
authors.lastname: Planta
authors.forename: W H
authors.initials: WH
authors.lastname: Mager
mesh_terms: D000222:Adaptation, Physiological / Q000235:genetics; D001483:Base Sequence; D015180:Electrophoresis, Gel, Two-Dimensional; D005656:Fungal Proteins / Q000032:analysis; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects; D008715:Methionine / Q000378:metabolism; D008969:Molecular Sequence Data; D009997:Osmotic Pressure / Q000187:drug effects; D012331:RNA, Fungal / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics* / Q000502:physiology*; D012965:Sodium Chloride / Q000494:pharmacology; D013395:Sucrose / Q000494:pharmacology; D013462:Sulfur Radioisotopes
chemical_list: D005656:Fungal Proteins; D012331:RNA, Fungal; D012333:RNA, Messenger; D013462:Sulfur Radioisotopes; D012965:Sodium Chloride; D013395:Sucrose; D008715:Methionine