[Quantitative analysis of glutathione and glutathione S-transferase in human brain tumors, C6 rat glioma cells and drug resistant C6 cells].
Glutathione (GSH) and Glutathione S-transferase (GST) plays an important role in the protection of cells against damage from free radicals and also influences cytotoxicity to some kinds of chemotherapeutic agents. GST comprises a group of abundant and widely distributed catalytic and binding proteins that facilitate the conjugation of GSH with the electrophilic center of a large spectrum of hydrophilic molecules. Multiple GST isozymes in mammalian tissues arise from dimeric combination of a number of distinct subunits grouped into three major classes: alpha (alpha), mu (mu), and pi (p). We report the total GST, GST-p activity and GSH content of human brain tumors, C6 rat glioma cells and drug resistant C6 cells. The values of total GST activity in 42 normal brain and brain tumors were quantitatively analyzed. Total GST activity was 92.6 +/- 25.1 units (mean +/- standard deviation) in 8 samples of normal brain tissues, 126 +/- 58.8 units in five grade II or III astrocytomas (154 +/- 63.3 units in grade II astrocytomas, 84.4 +/- 2.7 units in 2 grade III astrocytoma), 66.2 +/- 29.3 in 5 glioblastoma cases, 94.7 +/- 47.7 units in 3 metastatic tumors, 302 +/- 114 unit in 8 meningiomas and 213 +/- 90.4 units in 3 neurinomas. Differences of GST activity between glioblastomas and meningiomas, grade II or III astrocytomas and meningioma, in normal brain tissues and meningioma were statistically significant (p < 0.01). The difference between normal brain tissues and benign tumors (meningiomas and neurinomas), gliomas and benign tumors were also statistically significant (p < 0.05).(ABSTRACT TRUNCATED AT 250 WORDS)
Matsumoto
Y
Y
Department of Neurological Surgery, Kagawa Medical School.
Sasaoka
N
N
Tsuchida
T
T
Fujiwara
T
T
Nagao
S
S
D000818:Animals; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000494:pharmacology; D001932:Brain Neoplasms / Q000737:chemistry* / Q000201:enzymology / Q000473:pathology; D002945:Cisplatin / Q000494:pharmacology; D004351:Drug Resistance; D005910:Glioma / Q000737:chemistry* / Q000201:enzymology / Q000473:pathology; D005978:Glutathione / Q000032:analysis*; D005982:Glutathione Transferase / Q000032:analysis*; D015376:Nimustine / Q000494:pharmacology; D051381:Rats; D014407:Tumor Cells, Cultured; D014750:Vincristine / Q000494:pharmacology
D015376:Nimustine; D014750:Vincristine; D005982:Glutathione Transferase; D005978:Glutathione; D002945:Cisplatin