Transcription of human respiratory syncytial virus genome RNA in vitro: requirement of cellular factor(s).
Extracts made from human respiratory syncytial virus (RSV)-infected Hep-2 cells synthesized mRNAs encoded by all known viral genes. In contrast, RSV ribonucleoproteins purified from infected cells failed to transcribe in vitro; transcription was restored by addition of a cytoplasmic extract of uninfected Hep-2 cells, demonstrating that a cellular factor(s) has a role in RSV gene expression. Quantitation of the individual gene mRNAs transcribed in vitro revealed polarity of transcription of the genome.
Barik
S
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Department of Molecular Biology, Cleveland Clinic Foundation, Ohio 44195-5069.
D002474:Cell-Free System; D005814:Genes, Viral / Q000235:genetics; D016679:Genome, Viral; D006801:Humans; D012333:RNA, Messenger / Q000096:biosynthesis; D012367:RNA, Viral / Q000235:genetics*; D012136:Respiratory Syncytial Viruses / Q000235:genetics*; D012261:Ribonucleoproteins / Q000378:metabolism; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured
D012333:RNA, Messenger; D012367:RNA, Viral; D012261:Ribonucleoproteins