Differences in HTLV-I integration patterns between skin lesions and peripheral blood lymphocytes of HTLV-I seropositive patients with cutaneous lymphoproliferative disorders.
We examined HTLV-I integration patterns in nine cases of HTLV-I-seropositive patients with cutaneous lymphoproliferative disorders. The Southern blot on EcoRI digests of DNA revealed a discrete band of HTLV-I provirus (monoclonal integration) in either skin lesions or peripheral blood lymphocytes (PBL). Four cases showed the monoclonal integration of HTLV-I provirus only in skin lesions: one case showed only in PBL and two cases showed in both skin and PBL. The Southern blot on PstI digests of DNA revealed a 2.4 Kb band of the internal construct of HTLV-I provirus (polyclonal integration) in the PBL of EcoRI-negative samples. The difference in HTLV-I integration patterns between skin lesions and PBL in these cases suggests that the monoclonal outgrowth of HTLV-I-infected cells in the skin is causatively associated with the pathogenesis of cutaneous ATL.
Hamada
T
T
Department of Dermatology, Faculty of Medicine, Kagoshima University, Japan.
Setoyama
M
M
Katahira
Y
Y
Furuno
T
T
Fujiyoshi
T
T
Sonoda
S
S
Tashiro
M
M
D000368:Aged; D005260:Female; D015481:HTLV-I Antibodies / Q000097:blood; D015368:Human T-lymphotropic virus 1 / Q000235:genetics / Q000502:physiology*; D006801:Humans; D015459:Leukemia-Lymphoma, Adult T-Cell / Q000276:immunology / Q000382:microbiology* / Q000473:pathology; D008214:Lymphocytes / Q000382:microbiology; D008232:Lymphoproliferative Disorders / Q000276:immunology / Q000382:microbiology / Q000473:pathology; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012867:Skin / Q000276:immunology / Q000382:microbiology* / Q000473:pathology; D016662:Virus Integration
D015481:HTLV-I Antibodies