Detection of specific anti-Giardia antibodies in the serodiagnosis of symptomatic giardiasis.
The roles of circulating anti-Giardia IgM and IgG antibodies were assayed by the Enzyme linked immunosorbent assay (ELISA) using axenic Giardia lamblia whole trophozoites as antigen for routine serodiagnosis of Giardia infection. Detection of anti-Giardia IgM antibody was found to be useful in the early detection of symptomatic giardiasis, whereas IgG detection was not, since the latter could not discriminate between present infection and previous exposure. A mean optical density (OD) +/- 2 standard deviation (SD) value of 0.088 at 1:100 serum dilution was found optimal for the detection of anti-Giardia IgM antibodies as it completely separated out the acute Giardia cases from the Giardia-free controls. The same sera did not reveal any marked differences when tested for anti-Giardia IgG antibodies. Detection of IgM antibodies correlated with high sensitivity and specificity (96%) in an evaluation of a large number of sera collected from Giardia cases and controls.
Chaudhuri
P P
PP
Department of Parasitology, National Institute of Cholera and Enteric Diseases, Beliaghata, Calcutta, India.
Sengupta
K
K
Manna
B
B
Saha
M K
MK
Pal
S C
SC
Das
P
P
D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay; D005872:Giardia / Q000276:immunology*; D005873:Giardiasis / Q000175:diagnosis*; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood
D000913:Antibodies, Protozoan; D007074:Immunoglobulin G; D007075:Immunoglobulin M