Rabbit single domain antibodies specific to protein C expressed in prokaryotes.
VDJ genes were amplified by the polymerase chain reaction from mRNA isolated from peripheral blood B cells of rabbits immunized with protein C. The amplified genes were cloned into a lambda phage expression vector and packaged. A library of 6 x 10(5) recombinant phages was screened with labelled protein C and 30 positive clones were found. Three of them were plaque purified and the affinity of the single domain antibodies to the antigen determined to be 10(6)-10(7) l M-1. The data indicate the feasibility of generating single domain antibody, specific to protein antigen, from rabbit.
Suter
M
M
Schweizerisches Institut für Allergie- und Asthmaforschung, Davos, Switzerland.
Blaser
K
K
Aeby
P
P
Crameri
R
R
D000818:Animals; D000906:Antibodies / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D000918:Antibody Specificity; D001483:Base Sequence; D004926:Escherichia coli / Q000235:genetics; D005240:Feasibility Studies; D005784:Gene Amplification; D005803:Genes, Immunoglobulin; D007144:Immunoglobulin J-Chains / Q000235:genetics; D007135:Immunoglobulin Variable Region / Q000235:genetics; D007138:Immunoglobulin delta-Chains / Q000235:genetics; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011486:Protein C / Q000276:immunology*; D011817:Rabbits / Q000276:immunology*; D011993:Recombinant Fusion Proteins / Q000276:immunology*; D013045:Species Specificity
D000906:Antibodies; D007144:Immunoglobulin J-Chains; D007135:Immunoglobulin Variable Region; D007138:Immunoglobulin delta-Chains; D011486:Protein C; D011993:Recombinant Fusion Proteins