An evaluation of two simple synthetic peptide based anti-HIV assays.
Two simple synthetic peptide based assays for anti-HIV (Agen SimpliRED and Genetic Systems GENIE) were evaluated for their ability to distinguish samples that contained anti-HIV-1 from samples that did not. The anti-HIV negative samples were from uninfected subjects that had either given false positive reactions on existing screening assays or indeterminate reactivity on Western blot. The anti-HIV-1 positive samples had been shown to contain antibody by a number of assays, and clinical details of the patients were known. The SimpliRED and GENIE assays demonstrated similar performances, with sensitivities of 99.77% and 100%, and specificities of 97.78% and 99.16%, respectively.
Healey
D S
DS
National HIV Reference Laboratory, Fairfield Hospital, Vic Australia.
Dax
E M
EM
D000163:Acquired Immunodeficiency Syndrome / Q000175:diagnosis*; D005069:Evaluation Studies as Topic; D015483:HIV Antibodies / Q000032:analysis*; D015497:HIV-1; D006801:Humans; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000379:methods*
D015483:HIV Antibodies