Protein synthetic activity and adenylate energy charge in Rhein-treated cultured human glioma cells.
The effect of Rhein (RH) on the protein synthetic activity and adenylate energy charge in human glioma cells cultured in vitro has been investigated. The results demonstrate that in RH-treated cells, the protein synthesis is strongly decreased, but no modifications in the qualitative pattern occur. The extent of inhibition is a function of the drug concentration as well as of the time of exposure. Such an inhibition must be ascribed mainly to a reduction of adenylate energy charge brought about by RH because of its effect on respiration and glycolysis. The correlation between the adenylate energy charge and cell viability, as well as the possibility of using rhein as a biochemical modulator to reduce or to reverse multidrug resistance, are also discussed.
Delpino
A
A
Laboratory of Biophysics, Regina Elena Institute for Cancer Research, Rome, Italy.
Paggi
M G
MG
Gentile
P F
PF
Castiglione
S
S
Bruno
T
T
Benass
M
M
Floridi
A
A
D000227:Adenine Nucleotides / Q000378:metabolism*; D000244:Adenosine Diphosphate / Q000378:metabolism; D000249:Adenosine Monophosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000880:Anthraquinones / Q000494:pharmacology*; D004734:Energy Metabolism / Q000187:drug effects; D005910:Glioma / Q000188:drug therapy / Q000378:metabolism*; D006801:Humans; D009247:NADH, NADPH Oxidoreductases / Q000037:antagonists & inhibitors; D009363:Neoplasm Proteins / Q000096:biosynthesis*; D014407:Tumor Cells, Cultured
D000227:Adenine Nucleotides; D000880:Anthraquinones; D009363:Neoplasm Proteins; D000249:Adenosine Monophosphate; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D009247:NADH, NADPH Oxidoreductases; C020491:rhein