title: High resolution two-dimensional mapping of tissue-specific polypeptides in the desert locust, Schistocerca gregaria.
abstract: High resolution two-dimensional gel electrophoresis (2-DE) using immobilized pH gradients was used to map the tissue-specific polypeptides of the desert locust, Schistocerca gregaria. Highly specific comprehensive 2-DE reference maps ("master gels") were developed for the brain, corpus cardiacum, subesophageal ganglion, and hemolymph. The polypeptides were well resolved within the pH 4-7 range in the first dimension and within the 14-94 kDa molecular mass range in the second dimension.
authors.affiliation: Max-Planck-Institut für Biochemie, Martinsried, Germany.
authors.forename: R S
authors.initials: RS
authors.lastname: Annadurai
authors.forename: W
authors.initials: W
authors.lastname: Oberthür
authors.forename: H
authors.initials: H
authors.lastname: Rembold
mesh_terms: D000818:Animals; D001923:Brain Chemistry; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D005724:Ganglia / Q000737:chemistry; D006110:Grasshoppers / Q000737:chemistry*; D006458:Hemolymph / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing; D008970:Molecular Weight; D009490:Neurosecretory Systems / Q000737:chemistry; D010449:Peptide Mapping