title: Simplified methods for construction, assessment and rapid screening of peptide libraries in bacteriophage.
abstract: An efficient strategy has been devised for the construction of diverse peptide libraries in bacteriophage vectors. This strategy was used to generate a library of 4 x 10(8) random decapeptide inserts in the pIII protein of bacteriophage fd. A novel method for evaluating the genetic diversity of bacteriophage libraries based on colony hybridization with partially degenerate oligonucleotides has been developed. The decapeptide library was affinity-selected with a previously characterized monoclonal antibody specific for the V3 loop of the gp120 protein of HIV-1. Immunological screening, an efficient technique for the rapid identification of putative binding bacteriophage, is described. Hexapeptide sequences similar to those obtained from affinity selection of a hexapeptide bacteriophage library were obtained from the decapeptide library in all five frames. Immunological screening of 20,000 clones from the two libraries after two rounds of affinity selection rapidly identified antibody-binding sequences; 93% and 86% of the sequences obtained from the hexapeptide and decapeptide libraries, respectively, had IC50 values < or = 10 mM as free peptides.
authors.affiliation: Chiron Corporation, Emeryville, CA 94608.
authors.forename: R B
authors.initials: RB
authors.lastname: Christian
authors.forename: R N
authors.initials: RN
authors.lastname: Zuckermann
authors.forename: J M
authors.initials: JM
authors.lastname: Kerr
authors.forename: L
authors.initials: L
authors.lastname: Wang
authors.forename: B A
authors.initials: BA
authors.lastname: Malcolm
mesh_terms: D000595:Amino Acid Sequence; D001435:Bacteriophages / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods*; D004926:Escherichia coli / Q000235:genetics; D015723:Gene Library; D005822:Genetic Vectors / Q000235:genetics*; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000235:genetics; D009842:Oligopeptides / Q000235:genetics*; D010446:Peptide Fragments / Q000235:genetics*
chemical_list: D009838:Oligodeoxyribonucleotides; D009842:Oligopeptides; D010446:Peptide Fragments