A rapid chemiluminescent detection method for barley yellow dwarf virus.
Barley yellow dwarf virus (BYDV-PAV-IL) was detected with biotinylated in vitro transcript cDNA using a chemiluminescent substrate on nylon membranes. Signals were detected on X-ray film and quantified using either a densitometer or an ELISA plate reader. The time required for sample preparation was reduced so that the entire protocol could be completed in two days. The in vitro transcript probes could detect 1 ng of purified virus and as little as 1 microliter of sap extracts prepared from infected oat shoots.
Fouly
H M
HM
Department of Plant Pathology, University of Illinois, Urbana 61801.
Domier
L L
LL
D'Arcy
C J
CJ
D001467:Hordeum / Q000382:microbiology*; D008163:Luminescent Measurements; D010935:Plant Diseases / Q000382:microbiology; D010942:Plant Viruses / Q000302:isolation & purification*; D015347:RNA Probes; D012367:RNA, Viral / Q000302:isolation & purification*
D015347:RNA Probes; D012367:RNA, Viral