Conservation of bactericidal activity in ultrasound-exposed murine peritoneal phagocytic cells.
Murine peritoneal exudate cells (PEC), predominantly macrophages, were insonated in vitro with burst-mode ultrasound and assayed for their ability to phagocytose and kill Staphylococcus aureus. PEC were exposed at 37 degrees C in rotating tubes to 1-MHz, burst-mode (10 ms on, 10 ms off) ultrasound at 3.7 +/- 0.2 W/cm2 ISPTA (7.4 +/- 0.4 W/cm2 ISPBA) for 150 s. Bactericidal activity was assayed at 1, 2, and 3 h after exposure and subsequent 37 degrees C incubation with the bacteria for 20 min. In these experiments, which comprised 17 treated and 7 sham-treated control samples, there was no significant difference in results between treated and control samples (p > 0.29).
Prohaska
S S
SS
Cell and Molecular Biology Laboratory, SRI International, Menlo Park, CA 94025.
Rimer
V G
VG
Tusé
D
D
Pryor
G T
GT
Edmonds
P D
PD
D000818:Animals; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D010529:Peritoneal Cavity / Q000166:cytology*; D010586:Phagocytes / Q000502:physiology*; D013211:Staphylococcus aureus; D014465:Ultrasonics