title: Susceptibility testing of clinical isolates of Methylobacterium species.
abstract: Methylobacterium species represent a relatively new genus which is being increasingly isolated from cases of opportunistic infections. This study reports on 3 reference strains and 15 clinical isolates of Methylobacterium species. Susceptibility tests were performed by the agar dilution and commercial broth microdilution methods at both 30 and 35 degrees C. Readings were made at 24, 48, and 72 h. Incubation conditions of 48 h and 30 degrees C were found to be optimum. Both the agar dilution and broth microdilution methods gave equivalent results. Drugs tested and their MICs for 90% of isolates (in micrograms per milliliter) were as follows: amikacin, less than or equal to 1; gentamicin, 1; ciprofloxacin, 1; trimethoprim-sulfamethoxazole, 2/38; ceftriaxone, 16; and ceftizoxime, 16. The majority of our isolates were resistant to six other beta-lactam drugs tested. Nine of the 15 Methylobacterium isolates were beta-lactamase positive.
authors.affiliation: Department of Pathology, Wayne State University School of Medicine, Detroit, Michigan 48201.
authors.forename: W J
authors.initials: WJ
authors.lastname: Brown
authors.forename: R L
authors.initials: RL
authors.lastname: Sautter
authors.forename: A E
authors.initials: AE
authors.lastname: Crist
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D006088:Gram-Negative Aerobic Bacteria / Q000187:drug effects* / Q000201:enzymology; D006801:Humans; D008826:Microbial Sensitivity Tests; D001618:beta-Lactamases / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D001618:beta-Lactamases