3T3 cell surface galactosyltransferase is a calcium-dependent adhesion molecule for collagen type IV.
Cell surface galactosyltransferase (GalTase) has been previously shown to mediate cell spreading or migration on laminin matrices. This work demonstrates that 3T3 cell surface GalTase also mediates cell attachment to collagen type IV. Attachment to collagen type IV was blocked by perturbations of GalTase or substrate pregalactosylation on cells possessing only calcium-dependent mechanisms of adhesion. Cells with both calcium-dependent and calcium-independent systems were not affected by GalTase perturbation. Collagen type IV was shown to possess GalTase substrates since matrices could be galactosylated by both soluble enzyme and 3T3 cells.
Babiarz
B
B
Department of Biological Sciences, Rutgers University, Piscataway, New Jersey 08855-1059.
Cullen
E
E
D016475:3T3 Cells; D000818:Animals; D002118:Calcium / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D002462:Cell Membrane / Q000201:enzymology*; D003094:Collagen / Q000378:metabolism*; D005109:Extracellular Matrix / Q000502:physiology; D005700:Galactosyltransferases / Q000378:metabolism*; D007700:Kinetics; D007797:Laminin / Q000378:metabolism; D051379:Mice
D015815:Cell Adhesion Molecules; D007797:Laminin; D003094:Collagen; D005700:Galactosyltransferases; D002118:Calcium