Reduced binding activity of transcription factor NF-kappa B accounts for MHC class I repression in adenovirus type 12 E 1-transformed cells.
The early region 1 (E1) of human adenovirus (Ad) type 12 represses the expression of major histocompatibility (MHC) Class I genes in transformed primary rodent cells. In this paper we show that both NF-kappa B and KBF1 (p50 dimer) binding activity to the H2TF1 element in the Class I promoter is reduced in Ad12-13S-E1A-transformed cells compared to Ad5E1- or Ad12-12S-E1A-transformed cells. Consistently, in Ad12E1A-13S-transformed cells the H2TF1 element does not contribute to transcriptional activity in transient expression assays, whereas it does contribute in Ad12E1A-12S-transformed cells. Therefore, the most likely explanation is that reduced binding of NF-kappa B and KBF1 to the H2TF1 element accounts for the down-regulation of MHC Class I expression in Ad12E1- and Ad12E1A-13S-transformed cells.
Meijer
I
I
Laboratory for Molecular Carcinogenesis, Sylvius Laboratory, University of Leiden, The Netherlands.
Boot
A J
AJ
Mahabir
G
G
Zantema
A
A
van der Eb
A J
AJ
D017498:Adenovirus E1 Proteins / Q000032:analysis / Q000633:toxicity*; D001483:Base Sequence; D002472:Cell Transformation, Viral; D005805:Genes, MHC Class I; D005809:Genes, Regulator; D006183:H-2 Antigens / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D016328:NF-kappa B / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D014158:Transcription, Genetic
D017498:Adenovirus E1 Proteins; D006183:H-2 Antigens; C032876:H-2Kb protein, mouse; D016328:NF-kappa B