title: A rapid in situ respiration test for measuring aerobic biodegradation rates of hydrocarbons in soil.
abstract: An in situ test method to measure the aerobic biodegradation rates of hydrocarbons in contaminated soil is presented. The test method provides an initial assessment of bioventing as a remediation technology for hydrocarbon-contaminated soil. The in situ respiration test consists of ventilating the contaminated soil of the unsaturated zone with air and periodically monitoring the depletion of oxygen (O2) and production of carbon dioxide (CO2) over time after the air is turned off. The test is simple to implement and generally takes about four to five days to complete. The test was applied at eight hydrocarbon-contaminated sites of different geological and climatic conditions. These sites were contaminated with petroleum products or petroleum fuels, except for two sites where the contaminants were primarily polycyclic aromatic hydrocarbons. Oxygen utilization rates for the eight sites ranged from 0.02 to 0.99 percent O2/hour. Estimated biodegradation rates ranged from 0.4 to 19 mg/kg of soil/day. These rates were similar to the biodegradation rates obtained from field and pilot studies using mass balance methods. Estimated biodegradation rates based on O2 utilization were generally more reliable (especially for alkaline soils) than rates based on CO2 production. CO2 produced from microbial respiration was probably converted to carbonate under alkaline conditions.
authors.affiliation: Battelle, Columbus, Ohio.
authors.forename: R E
authors.initials: RE
authors.lastname: Hinchee
authors.forename: S K
authors.initials: SK
authors.lastname: Ong
mesh_terms: D000332:Aerobiosis; D001673:Biodegradation, Environmental; D002245:Carbon Dioxide / Q000032:analysis; D006838:Hydrocarbons / Q000032:analysis*; D010100:Oxygen / Q000032:analysis; D012987:Soil / Q000032:analysis*
chemical_list: D006838:Hydrocarbons; D012987:Soil; D002245:Carbon Dioxide; D010100:Oxygen