Characterization of Ca(2+)-ATPase of Setaria cervi (Nematoda: Filarioidea): effect of phenothiazines and anthelmintics.
1. A Mg2+ independent, Ca(2+)-ATPase requiring high concentrations of Ca2+ (5 mM) for the activation, equally distributed in cuticle-muscular-hypodermis, genital organs and gastrointestinal tissues and mainly localized in 10,000 g pellet fraction, was identified in Setaria cervi, a bovine filarial parasite. 2. Filarial enzyme showed Km value of 3.33 mM for ATP as computed from the double reciprocal Lineweaver-Burk plot. 3. The enzyme could be completely solubilized by sonication with about 4-fold increase in specific activity of the enzyme. 4. The enzyme showed about 2-fold activation by the calmodulin fractions isolated from S. cervi and rat brain homogenates. 5. The enzyme was highly sensitive to inhibition with some phenothiazine derivatives. Trifluoperazine was observed to be the most potent inhibitor followed by promethazine and chlorpromazine. 6. Some anthelmintics viz. diethycarbamazine and centperazine were found to be highly potent inhibitors of the enzyme, significant inhibition of filarial Ca(2+)-ATPase was also observed with levamisole and suramine. 7. Studies indicate Ca(2+)-ATPase of S. cervi as a potential chemotherapeutic target.
Agarwal
A
A
Division of Biochemistry, Central Drug Research Institute, Lucknow, India.
Shukla
O P
OP
Tekwani
B L
BL
D000818:Animals; D000871:Anthelmintics / Q000494:pharmacology*; D002122:Calcium Chloride / Q000494:pharmacology; D000252:Calcium-Transporting ATPases / Q000187:drug effects / Q000378:metabolism*; D002147:Calmodulin / Q000378:metabolism; D004533:Egtazic Acid / Q000494:pharmacology; D005260:Female; D005370:Filarioidea / Q000187:drug effects / Q000201:enzymology*; D007700:Kinetics; D010640:Phenothiazines / Q000494:pharmacology*; D012719:Setariasis; D012995:Solubility
D000871:Anthelmintics; D002147:Calmodulin; D010640:Phenothiazines; D004533:Egtazic Acid; D000252:Calcium-Transporting ATPases; D002122:Calcium Chloride