Assessment of sperm for cryopreservation using the hypoosmotic viability test.
In summary, the hypoosmotic viability parameter was significantly correlated with the outcome of the thawed sperm motility. The prefreeze supravital staining for sperm viability and the hypoosmotic sperm swelling test were not predictive of the thawed sperm total motility. The hypoosmotic viability parameter was not correlated to the postwarmed sperm motility after refrigeration. The results indicated that the integrity of the sperm membranes at the head were more important than the tail membrane.
Chan
P J
PJ
Department of Obstetrics and Gynecology, Loma Linda University School of Medicine, California 92350.
Tredway
D R
DR
Pang
S C
SC
Corselli
J
J
Su
B C
BC
D002470:Cell Survival; D015925:Cryopreservation; D006801:Humans; D008297:Male; D009995:Osmosis; D013081:Sperm Motility; D013094:Spermatozoa / Q000502:physiology*