Fluorescence of chromatin DNA by an oxazolium scintillator.
Aqueous solutions of a yellow and highly fluorescent derivative from dimethyl-POPOP, obtained by treating a chloroform solution of this oxazole scintillator with dimethylsulfate, induce a strong blue-green or yellow-green fluorescence in chromatin DNA under ultraviolet (365 nm) or violet-blue (436 nm) excitation, respectively. It is suggested that this new and selective fluorescence reaction could originate from binding of the oxazolium derivative into the minor groove of DNA.
Stockert
J C
JC
Max-Planck-Institut für Biologie, Abteilung Mikrobiologie, Tübingen, Bundesrepublik Deutschland.
D000818:Animals; D001773:Blood Cells / Q000166:cytology*; D002645:Chickens; D002843:Chromatin / Q000648:ultrastructure*; D004247:DNA / Q000032:analysis*; D005456:Fluorescent Dyes; D005881:Gingiva / Q000166:cytology*; D006801:Humans; D008856:Microscopy, Fluorescence / Q000379:methods; D010080:Oxazoles; D051381:Rats; D014349:Trypanosoma cruzi / Q000166:cytology*
D002843:Chromatin; D005456:Fluorescent Dyes; D010080:Oxazoles; C007478:dimethyl-POPOP; D004247:DNA