The extracellular matrix of cerebral gray matter: Golgi's pericellular net and Nissl's nervösen grau revisited.
Glial hyaluronate-binding protein (GHAP) and a large aggregating chondroitin sulfate proteoglycan (Ag-Pg) similar to a fibroblast proteoglycan (versican) were localized in bovine, dog and cat central nervous system (CNS) gray matter by indirect immunofluorescence. The distribution of the two hyaluronate-binding proteins was identical with that of hyaluronate, an extracellular glycosaminoglycan. All substances formed a finely reticulated mesh in the neuropil with a condensation of the stain around large neurons. It is concluded that in gray matter, as in white matter, the extracellular matrix (ECM) contains hyaluronate-protein aggregates. We suggest that the hyaluronate-protein aggregates correspond to the pericellular network first described by Golgi.
Bignami
A
A
Department of Pathology, Harvard Medical School, Boston, MA.
Asher
R
R
Perides
G
G
Rahemtulla
F
F
D000818:Animals; D001921:Brain / Q000378:metabolism*; D002352:Carrier Proteins / Q000276:immunology / Q000378:metabolism; D002415:Cats; D002417:Cattle; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004285:Dogs; D005109:Extracellular Matrix / Q000378:metabolism* / Q000648:ultrastructure; D005455:Fluorescent Antibody Technique; D006056:Golgi Apparatus / Q000378:metabolism* / Q000648:ultrastructure; D006820:Hyaluronic Acid / Q000276:immunology / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000276:immunology / Q000378:metabolism; D011485:Protein Binding; D011509:Proteoglycans / Q000276:immunology / Q000378:metabolism
D002352:Carrier Proteins; D009419:Nerve Tissue Proteins; D011509:Proteoglycans; D006820:Hyaluronic Acid