Culture of postimplantation rat embryos in rabbit serum for the identification of the growth factor in fractionated rat serum.
The growth factor for postimplantation rat embryos was investigated on the basis of the serum species-specificity in supporting embryonic development in culture. We used rabbit serum as a basal medium for the culture of head-fold stage rat embryos, and examined the effects of various fractions of rat serum on their development. In rabbit serum alone, rat embryos developed poorly. With the rat serum ultrafiltrate of molecular weight (MW) < 300,000, embryonic development improved, but not with the ultrafiltrate of MW < 100,000. With dialyzed rat serum or the globulin fraction of rat serum, embryonic development improved, but the albumin fraction had no effect. It was concluded from these results that some macromolecular growth factor for cultured postimplantation rat embryos was present in the globulin fraction of rat serum. The molecular weight of this growth factor was estimated to be between 65,000 and 300,000. Rabbit serum was considered to be suitable as a medium for the identification of this growth factor.
Usami
M
M
Division of Pharmacology, National Institute of Hygienic Sciences, Tokyo, Japan.
Nakaura
S
S
Kawashima
K
K
Tanaka
S
S
Takanaka
A
A
D000818:Animals; D046508:Culture Techniques; D005314:Embryonic and Fetal Development / Q000502:physiology*; D006133:Growth Substances / Q000097:blood* / Q000502:physiology; D011817:Rabbits; D051381:Rats; D013045:Species Specificity
D006133:Growth Substances