Bispecific IgA/IgM antibodies and their use in enzyme immunoassay.
Two hybrid hybridomas secreting polymeric bispecific antibodies to human chorionic gonadotropin and calf intestinal alkaline phosphatase were produced by fusion of IgA- and IgM-secreting mouse hybridomas. Both hybrid antibodies were purified from ascitic fluid by size exclusion chromatography. An IgM-like fraction was shown to exhibit bispecific activity. Bispecificity was completely lost following mild reduction and alkylation. Both bispecific antibodies were used to develop a sensitive enzyme immunoassay for hCG.
Behrsing
O
O
Max Delbrück Centre for Molecular Medicine, Berlin-Buch, Germany.
Kaiser
G
G
Karawajew
L
L
Micheel
B
B
D000469:Alkaline Phosphatase / Q000276:immunology; D000478:Alkylation; D000818:Animals; D000918:Antibody Specificity; D006063:Chorionic Gonadotropin / Q000032:analysis* / Q000276:immunology; D006801:Humans; D006822:Hybrid Cells; D007124:Immunoenzyme Techniques; D007070:Immunoglobulin A / Q000737:chemistry*; D007075:Immunoglobulin M / Q000737:chemistry*; D051379:Mice; D010084:Oxidation-Reduction; D011993:Recombinant Fusion Proteins
D006063:Chorionic Gonadotropin; D007070:Immunoglobulin A; D007075:Immunoglobulin M; D011993:Recombinant Fusion Proteins; D000469:Alkaline Phosphatase