title: Effect of transmural vagal stimulation on electrotonic current spread in the rabbit sinoatrial node.
abstract: OBJECTIVE
  The effect of vagal stimulation on the decay of electrotonic potential caused by intracellular current injection and on input resistance was measured in the sinoatrial node of isolated rabbit right atria.
  
  
  METHODS
  Studies were performed on New Zealand White rabbits weighing approximately 2-3 kg. Vagal stimulation was achieved by transmural stimulation of intramural nerve fibres in the presence of propranolol. A K+ perfused suction electrode was used to inject hyperpolarising current pulses; input resistance was measured by means of a double barrel microelectrode.
  
  
  RESULTS
  Vagal stimulation which caused a 14-20% increase of cycle length diminished electronic potential significantly by a decrease of membrane resistance. The input resistance of the sinoatrial node was not affected. Space constant values calculated by using either a one or a two dimensional model of electrotonic current spread were decreased on average by 13% and 14% respectively.
  
  
  CONCLUSIONS
  The results from this study show that vagal stimulation which gave rise to a moderate negative chronotropic effect and marked changes in action potential configuration of nodal fibres affects the electrotonic interaction within the sinoatrial node. This may have consequences for the electrical activity and synchronisation of the sinoatrial nodal fibres.
authors.affiliation: Department of Physiology, University of Amsterdam, The Netherlands.
authors.forename: J J
authors.initials: JJ
authors.lastname: Duivenvoorden
authors.forename: L N
authors.initials: LN
authors.lastname: Bouman
authors.forename: T
authors.initials: T
authors.lastname: Opthof
authors.forename: F F
authors.initials: FF
authors.lastname: Bukauskas
authors.forename: H J
authors.initials: HJ
authors.lastname: Jongsma
mesh_terms: D000200:Action Potentials / Q000187:drug effects / Q000502:physiology; D000818:Animals; D004553:Electric Conductivity / Q000187:drug effects / Q000502:physiology; D004558:Electric Stimulation / Q000379:methods; D005260:Female; D008297:Male; D011433:Propranolol / Q000494:pharmacology; D011817:Rabbits; D012849:Sinoatrial Node / Q000502:physiology*; D014630:Vagus Nerve / Q000187:drug effects / Q000502:physiology*
chemical_list: D011433:Propranolol