Prostanoid-induced inhibition of lipolysis in rat isolated adipocytes: probable involvement of EP3 receptors.
Sulprostone, enprostil and 16, 16 dimethyl PGE2 have all been found to be potent inhibitors of lipolysis induced by 3-isobutyl-1-methyl-xanthine (IBMX) in rat isolated adipocytes. The potency of sulprostone and enprostil in particular indicates that the response is likely to be mediated through either EP3 or EP1-receptors. However, the EP1-receptor blocking drug, AH6809, had no effect on the antilipolytic response to either PGE2 or sulprostone. We therefore conclude that the receptors mediating prostanoid-induced inhibition of lipolysis in rat adipocytes must principally be of the EP3 sub-type.
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Pharmacology Division, Glaxo Group Research Ltd. Ware, Hertfordshire, UK.
Coleman
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Humphrey
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D000273:Adipose Tissue / Q000166:cytology / Q000187:drug effects*; D000818:Animals; D015232:Dinoprostone / Q000031:analogs & derivatives* / Q000037:antagonists & inhibitors / Q000494:pharmacology; D016620:Enprostil / Q000494:pharmacology*; D066298:In Vitro Techniques; D008066:Lipolysis / Q000187:drug effects*; D011448:Prostaglandin Antagonists / Q000494:pharmacology; D011459:Prostaglandins E, Synthetic / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D011982:Receptors, Prostaglandin / Q000502:physiology*; D014966:Xanthenes / Q000494:pharmacology; D044004:Xanthones
D011448:Prostaglandin Antagonists; D011459:Prostaglandins E, Synthetic; D011982:Receptors, Prostaglandin; D014966:Xanthenes; D044004:Xanthones; C053876:6-isopropoxy-9-oxoxanthene-2-carboxylic acid; C017244:16,16-dimethylprostaglandin E; C016767:sulprostone; D016620:Enprostil; D015232:Dinoprostone