title: Benastatins A and B, new inhibitors of glutathione S-transferase, produced by Streptomyces sp. MI384-DF12. I. Taxonomy, production, isolation, physico-chemical properties and biological activities.
abstract: Benastatins have been isolated as part of a program designed to find microorganism-produced inhibitors of glutathione S-transferase from Streptomyces sp. MI384-DF12. They were purified by chromatography of reversed-phase silica gel, silica gel and Capcell Pak C18 (HPLC) followed by solvent extraction and then isolated as yellow powders. Benastatins A and B have the molecular formulae, C30H28O7 and C30H30O7, respectively. They were competitive with 3,4-dichloronitrobenzene as the substrate, and the inhibition constants (Ki) of benastatins A and B were 5.0 x 10(-6) and 3.7 x 10(-6), respectively.
authors.affiliation: Institute of Microbial Chemistry, Tokyo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Aoyagi
authors.forename: T
authors.initials: T
authors.lastname: Aoyama
authors.forename: F
authors.initials: F
authors.lastname: Kojima
authors.forename: N
authors.initials: N
authors.lastname: Matsuda
authors.forename: M
authors.initials: M
authors.lastname: Maruyama
authors.forename: M
authors.initials: M
authors.lastname: Hamada
authors.forename: T
authors.initials: T
authors.lastname: Takeuchi
mesh_terms: D000818:Animals; D001551:Benz(a)Anthracenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D005982:Glutathione Transferase / Q000037:antagonists & inhibitors*; D006094:Gram-Positive Bacteria / Q000187:drug effects; D051379:Mice; D008826:Microbial Sensitivity Tests; D009279:Naphthacenes; D051381:Rats; D013302:Streptomyces / Q000737:chemistry* / Q000235:genetics
chemical_list: D001551:Benz(a)Anthracenes; D004791:Enzyme Inhibitors; D009279:Naphthacenes; C077629:benastatin A; C077630:benastatin B; D005982:Glutathione Transferase