Calretinin in rat ovary: an in situ hybridization and immunohistochemical study.
Calretinin is a cytosolic calcium-binding protein of the calmodulin superfamily, with high homology with calbindin D28k. The only cells in which calretinin has been described so far are neurons, in the central nervous system and in retina. In the present work, we describe the expression of the calretinin gene in the interstitial cells of rat ovary. Immunohistochemistry, using a calretinin-specific antibody, allowed to detect the protein from 19 days after birth. Western blot from ovary homogenates confirmed the labelling of a 29 kDa band, the size of calretinin. In situ hybridization confirmed immunochemical data; calretinin transcripts were clearly shown in the same cell population. This represents the first description of calretinin outside the nervous system. Its function in ovary remains to be determined.
Pohl
V
V
Laboratoire d'Histologie Faculté de Médicine, Université Libre de Bruxelles, Belgium.
Van Rampelbergh
J
J
Mellaert
S
S
Parmentier
M
M
Pochet
R
R
D000818:Animals; D015153:Blotting, Western; D064032:Calbindin 2; D005260:Female; D015870:Gene Expression; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D010053:Ovary / Q000737:chemistry*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D064030:S100 Calcium Binding Protein G / Q000032:analysis* / Q000235:genetics / Q000276:immunology
C576512:Calb2 protein, rat; D064032:Calbindin 2; D012333:RNA, Messenger; D064030:S100 Calcium Binding Protein G