In vivo recombination and the production of hybrid genes.
In vivo recombination between homologous genes is increasingly being favoured as a means of generating proteins with altered and novel specificities. The typical procedure requires the cloning of two related genes on a single replicative plasmid of Escherichia coli and the selection or screening of recombinants. Up to now the recombination process between the cloned genes was generally thought to involve the recA function and the availability of free ends in the DNA molecule to be recombined. Our results show that neither is necessary. Recombinants are obtained by simply growing the bacteria that host the plasmid carrying the two cloned genes.
Calogero
S
S
Dipartimento di Genetica e Microbiologia, Adriano Buzzati-Traverso, Pavia, Italy.
Bianchi
M E
ME
Galizzi
A
A
D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial; D010957:Plasmids; D015202:Protein Engineering; D011938:Rec A Recombinases / Q000235:genetics; D011995:Recombination, Genetic
D011938:Rec A Recombinases