Localization and identification of actin structures involved in the filamin-actin interaction.
The interface between gizzard filamin and skeletal muscle actin was located on the actin monomer. Conserved sequences 105-120 and 360-372, in the actin subdomain 1 near the myosin binding sites, were involved in this interaction. The corresponding peptides for these sequences were each found to bind filamin and compete in the actin-filamin interaction. When these two peptides were used together in the presence of filamin and filamentous actin, they dissociated sedimentable complexes formed by these two proteins.
Méjean
C
C
UPR 8402 Centre de Recherches de Biochimie Macromoléculaire (Centre National de la Recherche Scientifique), U 249, Montpellier, France.
Lebart
M C
MC
Boyer
M
M
Roustan
C
C
Benyamin
Y
Y
D000199:Actins / Q000737:chemistry* / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism; D002645:Chickens; D003285:Contractile Proteins / Q000302:isolation & purification / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D064448:Filamins; D005456:Fluorescent Dyes; D007700:Kinetics; D008840:Microfilament Proteins / Q000302:isolation & purification / Q000378:metabolism*; D009130:Muscle, Smooth / Q000378:metabolism; D009132:Muscles / Q000378:metabolism; D009282:Naphthalenesulfonates; D010446:Peptide Fragments / Q000302:isolation & purification; D011485:Protein Binding; D011817:Rabbits; D014335:Tropomyosin / Q000378:metabolism
D000199:Actins; D002352:Carrier Proteins; D003285:Contractile Proteins; D064448:Filamins; D005456:Fluorescent Dyes; D008840:Microfilament Proteins; D009282:Naphthalenesulfonates; D010446:Peptide Fragments; D014335:Tropomyosin; C006421:1,5-I-AEDANS