In vitro bioassay for type E prostaglandins based on their NK immunodepressing activity.
A biological assay for PGE in commercial preparations, or secreted by tumor cells in culture fluid was developed on the basis of the immunosuppressive effect of PGE on the cytotoxic activity of NK cells. The assay is simple, rapid and convenient for detecting PGE cell secretion, either spontaneous or induced by various signals. The sensitivity of the bioassay is limited by the sensitivity of NK cells to the immunosuppressive activity of PGE (i.e., about 10(-8) M).
Kluchareva
T E
TE
All-Union Cancer Research Center, Moscow, Russia.
Matveeva
V A
VA
Kushlinsky
N E
NE
D000818:Animals; D001681:Biological Assay / Q000379:methods*; D006224:Cricetinae; D003601:Cytotoxicity Tests, Immunologic; D003602:Cytotoxicity, Immunologic / Q000187:drug effects; D015232:Dinoprostone / Q000494:pharmacology; D005069:Evaluation Studies as Topic; D006801:Humans; D007166:Immunosuppressive Agents / Q000032:analysis / Q000494:pharmacology; D066298:In Vitro Techniques; D007694:Killer Cells, Natural / Q000187:drug effects* / Q000276:immunology; D008647:Mesocricetus; D011458:Prostaglandins E / Q000032:analysis* / Q000378:metabolism / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000378:metabolism
D007166:Immunosuppressive Agents; D011458:Prostaglandins E; D015232:Dinoprostone