title: Developmental changes in rat liver mitochondrial populations analyzed by flow cytometry.
abstract: Isolated rat liver mitochondria were split into three density fractions when applied to a Percoll gradient. This phenomenon is observable in the fetus, in the early newborn (1 h), in the suckling newborn (7 days), and in the adult, suggesting that the three density fractions coexist regardless of the state of development. The medium-density fraction sharply decreased immediately after delivery, being replaced by the high-density fraction. Flow cytometry analysis of mitochondrial density fractions stained with rhodamine 123 showed the occurrence in each density fraction and in all developmental states studied of two distinct mitochondrial populations with different fluorescence intensities. Our results suggest that the high-fluorescence population might be an immature form of mitochondria that decreases with the progression of development, coinciding with the postnatal enhancement of mitochondrial respiratory efficiency.
authors.affiliation: Departamento de Bioquímica y Biologia Molecular, Facultad de Farmacia, Universidad de Salamanca, Spain.
authors.forename: C
authors.initials: C
authors.lastname: López-Mediavilla
authors.forename: A
authors.initials: A
authors.lastname: Orfao
authors.forename: J
authors.initials: J
authors.lastname: San Miguel
authors.forename: J M
authors.initials: JM
authors.lastname: Medina
mesh_terms: D000375:Aging / Q000502:physiology*; D000818:Animals; D000831:Animals, Newborn; D000833:Animals, Suckling; D002458:Cell Fractionation; D005260:Female; D005333:Fetus; D005434:Flow Cytometry; D005456:Fluorescent Dyes; D008099:Liver / Q000196:embryology / Q000254:growth & development* / Q000648:ultrastructure; D008930:Mitochondria, Liver / Q000648:ultrastructure*; D011247:Pregnancy; D051381:Rats; D017208:Rats, Wistar; D020112:Rhodamine 123; D012235:Rhodamines
chemical_list: D005456:Fluorescent Dyes; D012235:Rhodamines; D020112:Rhodamine 123