Structure of the tilapia (Oreochromis mossambicus) prolactin I gene.
The tilapia (Oreochromis mossambicus) prolactin-I (PRL-I) gene has been cloned and sequenced. Its transcript (3,677 bases long) begins with a guanine and is organized in five exons and four introns like the other known prolactin genes. Analysis of the 1,555-bp 5'-flanking region suggests that pituitary-specific expression of the gene could be regulated through a trans-factor related to the mammalian pituitary-specific factor Pit-1. Two potential binding sites for such a factor were found in the first intron, suggesting a possible regulatory role for this region. Moreover, two potential Z-DNA regions are located at positions -837 to -812 and -246 to -179 from the transcription start site. These two regions could play an important role in the regulation of PRL gene expression.
Swennen
D
D
Laboratoire de Biologie Moléculaire et de Génie Génétique, Université de Liège, Sart Tilman, Belgium.
Poncelet
A C
AC
Sekkali
B
B
Rentier-Delrue
F
F
Martial
J A
JA
Belayew
A
A
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004247:DNA; D006801:Humans; D008969:Molecular Sequence Data; D011388:Prolactin / Q000235:genetics*; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D017210:Tilapia; D014158:Transcription, Genetic
D011388:Prolactin; D004247:DNA