title: The human immunodeficiency virus tat protein increases the transcription of human Alu repeated sequences by increasing the activity of the cellular transcription factor TFIIIC.
abstract: The HIV Tat protein is able to upregulate the transcription by RNA polymerase III of cotransfected or endogenous cellular Alu-repeated sequences in both HeLa and Jurkat T cells. This effect is mediated by an increase in the activity of transcription factor TFIIIC, which binds to the B box in the RNA polymerase III Alu promoter. This is the first example of an effect of the Tat protein on the transcription of a cellular gene or on the activity of a cellular transcription factor. The significance of this effect for the life cycle of HIV and its interaction with infected cells is discussed.
authors.affiliation: Department of Biochemistry, University College and Middlesex School of Medicine, London, U.K.
authors.forename: K L
authors.initials: KL
authors.lastname: Jang
authors.forename: M K
authors.initials: MK
authors.lastname: Collins
authors.forename: D S
authors.initials: DS
authors.lastname: Latchman
mesh_terms: D000199:Actins / Q000235:genetics; D001483:Base Sequence; D002460:Cell Line; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D015967:Gene Expression Regulation, Viral; D015696:Gene Products, tat / Q000502:physiology*; D006678:HIV / Q000235:genetics*; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000737:chemistry; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid; D014157:Transcription Factors / Q000502:physiology*; D019474:Transcription Factors, TFIII; D014158:Transcription, Genetic; D014162:Transfection; D054322:tat Gene Products, Human Immunodeficiency Virus; D000098832:Transcription Factor TFIIIC
chemical_list: D000199:Actins; D004279:DNA, Viral; D015696:Gene Products, tat; D009841:Oligonucleotides; D012333:RNA, Messenger; D014157:Transcription Factors; D019474:Transcription Factors, TFIII; D054322:tat Gene Products, Human Immunodeficiency Virus; D000098832:Transcription Factor TFIIIC