title: Serum-free cell culture.
abstract: Cell culture is one important tool when studying cellular functions and molecular biology. It is also a basic method in most virological investigations. Serum has been an obligatory component in most cell culture media. During the last decades serum-free, chemically defined media have been developed, that are supplemented with a number of substances with specific cellular activities. The main developments of defined media are presented. Examples are given of investigations with different cell types.
authors.affiliation: National Bacteriological Laboratory, Stockholm, Sweden.
authors.forename: U
authors.initials: U
authors.lastname: Bjare
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D016895:Culture Media, Serum-Free; D006801:Humans
chemical_list: D016895:Culture Media, Serum-Free