Evaluation of an immunoenzymatic test (ELISA) for the diagnosis of leptospirosis in Italy.
An enzyme-linked immunosorbent assay (ELISA) was assessed to detect in humans IgM and IgG against the main serogroups/serovars of Leptospira present in Italy. Sonicated antigens from strain Wijnberg, serogroups Icterohaemorrhagiae and Riccio 2, serogroup Australis, were used in ELISA and the results were compared to microagglutination test (MAT). IgM were confirmed to be the predominant class of antibodies; the total sensitivity obtained by Wijnberg/ELISA with the sera of patients infected by different serogroups was 81.5% in relation to MAT, better sensitivity was achieved by the use of Riccio 2/ELISA; no reaction occurred with the control sera. Cross-reactivity was noted with some sera of patients with Lyme disease (IgM) and with few sera of patients with autoimmune disease (IgM and IgG). To improve the sensitivity of the ELISA both antigens Wijnberg and Riccio 2 must be employed in the immunoenzymatic assay, since serogroup Australis must be considered as a new emerging serogroup causing human leptospirosis in our country.
Cinco
M
M
Istituto di Microbiologia, Università degli Studi, Trieste, Italy.
Balanzin
D
D
Banfi
E
E
D000372:Agglutination Tests; D000907:Antibodies, Bacterial / Q000032:analysis*; D015153:Blotting, Western; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005069:Evaluation Studies as Topic; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D007558:Italy; D007919:Leptospira / Q000276:immunology; D007921:Leptospira interrogans / Q000276:immunology; D041341:Leptospira interrogans serovar canicola / Q000276:immunology; D007922:Leptospirosis / Q000175:diagnosis* / Q000276:immunology; D012967:Sodium Dodecyl Sulfate
D000907:Antibodies, Bacterial; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D012967:Sodium Dodecyl Sulfate