Some peptide-like colocalizations in endocrine cells of the pyloric caeca and the intestine of Oncorhynchus mykiss (Teleostei).
The coexistence of immunoreactivities to cholecystokinin, glucagon, glucagon-like peptide 1, salmon pancreatic polypeptide, neuropeptide tyrosine, and peptide tyrosine tyrosine was studied immunocytochemically, revealing for the first time in fish intestine the existence in the same cell of immunoreactivities to cholecystokinin-glucagon/glucagon-like peptide 1, cholecystokinin-salmon pancreatic polypeptide, glucagon/glucagon-like peptide 1-salmon pancreatic polypeptide, glucagon/glucagon-like peptide 1-neuropeptide tyrosine, salmon pancreatic polypeptide tyrosine tyrosine, and glucagon/glucagon-like peptide 1-peptide tyrosine tyrosine. Colocalization of cholecystokinin-salmon pancreatic polypeptide was observed only in the pyloric caeca of the rainbow trout Oncorhynchus mykiss, while the other colocalizations also occurred in proximal and middle intestinal segments. In all cases, endocrine cells immunoreactive to only one of the paired antisera were detected except for anti-glucagon and anti-glucagon-like peptide 1, which always immunostained the same cells.
Beorlegui
C
C
Departamento de Histología y Anatomía Patológica, Universidad de Navarra, Pamplona, Spain.
Martínez
A
A
Sesma
P
P
D000818:Animals; D002432:Cecum / Q000166:cytology / Q000378:metabolism; D002766:Cholecystokinin / Q000378:metabolism; D004702:Endocrine Glands / Q000166:cytology / Q000378:metabolism*; D005768:Gastrointestinal Hormones / Q000378:metabolism; D005934:Glucagon / Q000378:metabolism; D052216:Glucagon-Like Peptide 1; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000166:cytology; D009478:Neuropeptide Y / Q000378:metabolism; D010191:Pancreatic Polypeptide / Q000378:metabolism; D010446:Peptide Fragments / Q000378:metabolism; D019894:Peptide YY; D010455:Peptides / Q000378:metabolism*; D011498:Protein Precursors / Q000378:metabolism; D014337:Trout / Q000033:anatomy & histology / Q000378:metabolism*
D005768:Gastrointestinal Hormones; D009478:Neuropeptide Y; D010446:Peptide Fragments; D010455:Peptides; D011498:Protein Precursors; D019894:Peptide YY; D010191:Pancreatic Polypeptide; D052216:Glucagon-Like Peptide 1; D005934:Glucagon; D002766:Cholecystokinin