title: [Secretion of recombinant human epidermal growth factor into the periplasm in Escherichia coli cells].
abstract: Secretion vectors were constructed in which a synthetic gene of human epidermal growth factor (hEGF) joined with a gene coding for the leader peptide to one of the E. coli outer membrane major proteins (OmpF) is controlled by tac promoter. The increase of the hEGF yield was achieved by the multiplication of the gene copies. The hEGF in bacterial cells was secreted into periplasm. The recombinant protein was isolated by means of reverse phase chromatography as almost homogenous preparation (greater than 98%), the yield being 7 mg/l bacterial culture. The sequence of twenty-five N-terminal amino acid residues of the isolated hEGF coincided with that of the natural protein. The preparation proved to be biologically active.
authors.forename: N V
authors.initials: NV
authors.lastname: Batchikova
authors.forename: I B
authors.initials: IB
authors.lastname: Al'tman
authors.forename: S V
authors.initials: SV
authors.lastname: Lutsenko
authors.forename: V A
authors.initials: VA
authors.lastname: Smirnov
authors.forename: I V
authors.initials: IV
authors.lastname: Nazimov
authors.forename: L G
authors.initials: LG
authors.lastname: Eshkind
authors.forename: E A
authors.initials: EA
authors.lastname: Siniagina
authors.forename: A V
authors.initials: AV
authors.lastname: Azhaev
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000235:genetics; D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D004815:Epidermal Growth Factor / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics*; D005813:Genes, Synthetic; D006801:Humans; D008969:Molecular Sequence Data; D010957:Plasmids; D011401:Promoter Regions, Genetic; D021382:Protein Sorting Signals / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D001425:Bacterial Outer Membrane Proteins; D021382:Protein Sorting Signals; D011994:Recombinant Proteins; D004815:Epidermal Growth Factor
vernacular_title: Sekretsiia v periplazmu rekombinantnogo épidermal'nogo faktora rosta cheloveka v kletkakh Escherichia coli.