title: Heteronuclear NMR of DNA with the heteronucleus in natural abundance: facilitated assignment and extraction of coupling constants.
abstract: Two heteronuclear proton-carbon NMR experiments are applied to the DNA-octamer d(TTGGCCAA)2 with carbon in natural abundance. They lead to a complete assignment of the carbon resonances of the sugars and bases. In addition, several heteronuclear coupling constants, proton-carbon as well as proton-phosphorous and phosphorous-carbon, were determined. The information can be obtained in a reasonable measuring time and offers valuable information for a detailed picture of DNA structure.
authors.affiliation: Organisch-Chemisches Institut, TU München, Garching, Germany.
authors.forename: P
authors.initials: P
authors.lastname: Schmieder
authors.forename: J H
authors.initials: JH
authors.lastname: Ippel
authors.forename: H
authors.initials: H
authors.lastname: van den Elst
authors.forename: G A
authors.initials: GA
authors.lastname: van der Marel
authors.forename: J H
authors.initials: JH
authors.lastname: van Boom
authors.forename: C
authors.initials: C
authors.lastname: Altona
authors.forename: H
authors.initials: H
authors.lastname: Kessler
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*
chemical_list: D009838:Oligodeoxyribonucleotides; D004247:DNA