AKT2, a putative oncogene encoding a member of a subfamily of protein-serine/threonine kinases, is amplified in human ovarian carcinomas.
We isolated cDNA clones containing the entire coding region of the putative oncogene AKT2. Sequence analysis and in vitro translation demonstrated that AKT2 encodes a 56-kDa protein with homology to serine/threonine kinases; moreover, this protein contains a Src homology 2-like domain. AKT2 was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. AKT2 was mapped to chromosome region 19q13.1-q13.2 by fluorescence in situ hybridization. In the two ovarian carcinoma cell lines exhibiting amplification of AKT2, the amplified sequences were localized within homogeneously staining regions. We conclude that AKT2 belongs to a distinct subfamily of protein-serine/threonine kinases containing Src homology 2-like domains and that alterations of AKT2 may contribute to the pathogenesis of ovarian carcinomas.
Cheng
J Q
JQ
Department of Medical Oncology, Fox Chase Cancer Center, Philadelphia, PA 19111.
Godwin
A K
AK
Bellacosa
A
A
Taguchi
T
T
Franke
T F
TF
Hamilton
T C
TC
Tsichlis
P N
PN
Testa
J R
JR
D000595:Amino Acid Sequence; D002877:Chromosomes, Human / Q000648:ultrastructure; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D005456:Fluorescent Dyes; D005784:Gene Amplification; D015723:Gene Library; D006801:Humans; D008214:Lymphocytes / Q000166:cytology; D008969:Molecular Sequence Data; D005810:Multigene Family; D015513:Oncogene Proteins / Q000235:genetics*; D009857:Oncogenes; D010051:Ovarian Neoplasms / Q000201:enzymology / Q000235:genetics*; D014176:Protein Biosynthesis; D017346:Protein Serine-Threonine Kinases / Q000235:genetics*; D011518:Proto-Oncogene Proteins; D051057:Proto-Oncogene Proteins c-akt; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic
D005456:Fluorescent Dyes; D015513:Oncogene Proteins; D011518:Proto-Oncogene Proteins; D004247:DNA; C494921:AKT2 protein, human; D017346:Protein Serine-Threonine Kinases; D051057:Proto-Oncogene Proteins c-akt