M13 DNA fingerprinting, a new tool for classification and identification of Lactobacillus spp.
The optimal conditions for the application of M13 DNA fingerprinting to the genus Lactobacillus were determined. Comparative fingerprint analysis of representative strains of Lactobacillus delbrueckii subsp. delbrueckii, Lact. delbrueckii subsp. lactis, Lact. delbrueckii subsp. bulgaricus, Lact. helveticus and Lact. casei permitted the differentiation of species, subspecies and individual strains and the quantitative determination of their genetic relatedness. The results confirm the high specificity of M13 DNA fingerprinting and indicate that it might be used in the classification of Lactobacillus spp.
Miteva
V I
VI
Institute of Microbiology, Bulgarian Academy of Sciences, Sofia.
Abadjieva
A N
AN
Stefanova
T T
TT
D015139:Blotting, Southern; D016172:DNA Fingerprinting / Q000379:methods; D015342:DNA Probes; D004269:DNA, Bacterial / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D007778:Lactobacillus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D009693:Nucleic Acid Hybridization; D013045:Species Specificity
D015342:DNA Probes; D004269:DNA, Bacterial