The clonogen content of murine intestinal crypts: dependence on radiation dose used in its determination.
The number of colony-forming (clonogenic) cells in each of the crypts in mouse small intestine was deduced using a two-dose irradiation technique. The number was 7.5 +/- 0.8 cells using two equal doses each less than 9 Gy and 38 +/- 7 cells using 9 Gy or more per dose. The significant dose dependence could not be accounted for by considerations of intra- or intercrypt variability, or by the factor introduced to correct the sampling frequency for the influence of crypt size. The results suggest that more colony-forming cells may be recruited when the injury is more severe.
Hendry
J H
JH
Cancer Research Campaign Department of Experimental Radiation Oncology, Paterson Institute for Cancer Research, Christie Hospital (NHS) Trust, Manchester, United Kingdom.
Roberts
S A
SA
Potten
C S
CS
D000818:Animals; D003114:Colony-Forming Units Assay / Q000379:methods*; D004307:Dose-Response Relationship, Radiation; D007421:Intestine, Small / Q000166:cytology / Q000528:radiation effects*; D008297:Male; D051379:Mice; D013234:Stem Cells / Q000528:radiation effects*