Increased susceptibility of transfected prokaryotic and eukaryotic cells to antibiotic selection.
At 39 to 40 degrees C, selection of antibiotic-resistant transfected mammalian cell lines or Escherichia coli required lower aminoglycoside antibiotic concentrations than at 37 degrees C. The thermosensitivity of antibiotic susceptibility was much more manifest during genetic selection experiments than in conventional growth inhibition assays.
Asselbergs
F A
FA
Department of Biotechnology, Ciba-Geigy Ltd., Basel, Switzerland.
van Someren
P
P
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D016466:CHO Cells; D006224:Cricetinae; D004269:DNA, Bacterial / Q000187:drug effects / Q000235:genetics; D004926:Escherichia coli / Q000187:drug effects*; D005057:Eukaryotic Cells / Q000187:drug effects*; D011387:Prokaryotic Cells / Q000187:drug effects*; D013696:Temperature; D014162:Transfection
D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial