title: Crystallization and preliminary X-ray diffraction study of Lathyrus ochrus isolectin II complexed to the human lactotransferrin N2 fragment.
abstract: Isolectin II (LOL II) isolated from the seeds of Lathyrus ochrus has been crystallized in the presence of the N2 fragment (18,500 Da) isolated from human lactotransferrin, which contains an N-acetyllactosamine type biantennary glycan linked to Asn137. This is the first example of a legume lectin crystallized with an N-glycosylprotein. Crystals of the LOL II-N2 complex belong to the tetragonal space group (P4(1)2(1)2 or the enantiomorph) with cell dimensions: a = b = 63.5 A, c = 251.9 A. They diffract well up to at least 3.5 A resolution and more weakly up to 2.8 A resolution. Assuming one functional half-entity in the asymmetric unit, an alpha, beta monomer complexed to one N2 fragment (24,500 Da + 18,500 Da) would give a Vm of 2.95 A3/Da and a solvent content of approximately 58%. SDS/polyacrylamide gels of the dissolved crystals show the presence of both the LOL II and N2 fragment.
authors.affiliation: Laboratoire de Cristallographie et de Cristallisation des Macromolécules Biologiques, URA no. 1296 du CNRS, Faculté de Médecine Secteur-Nord, Marseille, France.
authors.forename: Y
authors.initials: Y
authors.lastname: Bourne
authors.forename: M P
authors.initials: MP
authors.lastname: Nésa
authors.forename: P
authors.initials: P
authors.lastname: Rougé
authors.forename: J
authors.initials: J
authors.lastname: Mazurier
authors.forename: D
authors.initials: D
authors.lastname: Legrand
authors.forename: G
authors.initials: G
authors.lastname: Spik
authors.forename: J
authors.initials: J
authors.lastname: Montreuil
authors.forename: C
authors.initials: C
authors.lastname: Cambillau
mesh_terms: D003460:Crystallization; D007887:Fabaceae / Q000737:chemistry*; D006801:Humans; D007781:Lactoferrin / Q000737:chemistry*; D037102:Lectins / Q000737:chemistry*; D046911:Macromolecular Substances; D037121:Plant Lectins; D010946:Plants, Medicinal; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D014961:X-Ray Diffraction
chemical_list: D037102:Lectins; D046911:Macromolecular Substances; D037121:Plant Lectins; D007781:Lactoferrin