[The characteristics and regulation of glutamate dehydrogenase in strains of Bacteroides ruminicola ssp. ruminicola isolated from the rumen of fallow deer].
Very little information about NH4+ assimilation paths in rumen anaerobic bacteria is available, and the information about wild animals is completely missing. Glutamate dehydrogenase (GDH) isolated from the rumen strain B. ruminicola in fallow deer was purified and its properties were specified after crystalline ammonium sulphate precipitation and gel filtration on Sephadex G-200. The properties of partly purified GDH were specified. One of the first specifications concerning GDH from various sources was to determine its coenzyme specificity. The results of these determinations enabled to draw a general conclusion that GDH from non-animal sources was specific to only one coenzyme while GDH from animal sources could utilize the two coenzymes (Frieden, 1964). In our study the specificity of GDH isolated from the rumen strain B. ruminicola in fallow deer to the coenzyme NADH (Tab. I) was determined; this specificity was different from the coenzyme specificity of GDH isolated from the rumen strain B. ruminicola in calves where GDH was found to be specific to the coenzyme NADPH. The effect of increasing concentrations of NADH, 2-oxoglutarate and NH4+ on the enzyme reaction velocity was also investigated and Km was determined for NADH, 2-oxoglutarate and NH4+ (Tab. II). The kinetic properties of GDH isolated from different sources are considerably variable. Michaelis constants for GDH range from 0.003 to 0.125 mmol/dm3 for NADPH (NADH), from 0.95 to 7.4 mmol/dm3 for 2-oxoglutarate, and from 0.25 to 16 mmol/dm3 for NH4+ (Misono et al., 1985). The average value of Km for NH4+ in a mixed rumen population was 33 mmol/dm3 (Erfle et al., 1977).(ABSTRACT TRUNCATED AT 250 WORDS)
Holovská
K
K
Univerzita veterinárskeho lekárstva, Kosice.
Lenártová
V
V
Rybosová
E
E
Orinák
A
A
Stachová
M
M
D000818:Animals; D001439:Bacteroides / Q000201:enzymology* / Q000302:isolation & purification; D003670:Deer / Q000382:microbiology*; D005969:Glutamate Dehydrogenase / Q000378:metabolism*; D012417:Rumen / Q000382:microbiology*
D005969:Glutamate Dehydrogenase
Vlastnosti a regulácia glutamátdehydrogenázy izolovanej z bachora kmena Bacteroides ruminicola ssp. ruminicola u daniela.