Enzyme immunoassay for specific analysis of pancreatic stone proteins in human pancreatic juice.
In order to study the concentration of pancreatic stone protein (PSP) in human pancreatic juice, we investigated the influence of the insoluble form of PSP-S1 converted from PSP-S2-5 on PSP determination and the assay method for PSP-S1 precipitate after solubilizing PSP-S1. When bovine trypsin was added to pancreatic juice, PSP-S1 was converted from PSP-S2-5 and precipitated about 45-85% after 1 h. The precipitated PSP-S1 was dissolved in 0.1 M sodium acetate buffer, pH 4.0, and the concentration was measured by the enzyme immunoassay, with similar reactivity to PSP-S1 and PSP-S2-5. The proposed method can offer accurate and specific analysis of the PSP level in pancreatic juice. The results of the fractionation of pancreatic juice and duodenal juice on Mono S cation-exchange chromatography suggested that the major component of PSP was PSP-S2-5 in pancreatic juice and PSP-S1 in duodenal juice.
Katsuzaki
T
T
Department of Clinical Chemistry, Maruko Pharmaceutical Co., Ltd., Kasugai, Japan.
Tatemichi
N
N
Takeichi
C
C
Hayakawa
S
S
Hayakawa
T
T
Shibata
T
T
Harada
H
H
Ochi
K
K
D002135:Calcium-Binding Proteins / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D002908:Chronic Disease; D004386:Duodenum / Q000737:chemistry; D005069:Evaluation Studies as Topic; D006801:Humans; D007124:Immunoenzyme Techniques; D007419:Intestinal Secretions / Q000737:chemistry; D051847:Lithostathine; D009419:Nerve Tissue Proteins; D010189:Pancreatic Juice / Q000737:chemistry*; D010195:Pancreatitis / Q000175:diagnosis / Q000378:metabolism; D012016:Reference Values; D012995:Solubility; D014357:Trypsin
D002135:Calcium-Binding Proteins; D051847:Lithostathine; D009419:Nerve Tissue Proteins; C497617:REG1A protein, human; D014357:Trypsin