title: In vitro study on the increase of trypsin resistance in the rat testicular sperm head.
abstract: The alteration of trypsin resistance in rat sperm head was investigated after the sperm were incubated in the culture media (199-Earele, 1% BSA) containing the inhibitors of protein synthesis. The head of non-incubated testicular sperm was easily digested by trypsin (1 mg/ml) while the head of the incubated sperm showed resistance to trypsin digestion after 3 hr-incubation. Trypsin resistance in epididymal sperm head did not change after the sperm was incubated. Neither cycloheximide (100 micrograms/ml) nor chloramphenicol (100 micrograms/ml) affected the trypsin resistance in the testicular sperm head.
authors.affiliation: Laboratory of Biology, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Matsuzawa
authors.forename: T
authors.initials: T
authors.lastname: Hoshino
authors.forename: J
authors.initials: J
authors.lastname: Ibuki
mesh_terms: D000818:Animals; D008297:Male; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D011506:Proteins / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013077:Sperm Head / Q000378:metabolism*; D013079:Sperm Maturation / Q000502:physiology; D013737:Testis / Q000166:cytology; D014357:Trypsin / Q000378:metabolism*
chemical_list: D011500:Protein Synthesis Inhibitors; D011506:Proteins; D014357:Trypsin