title: Comparison of protection from homologous cell-free vs cell-associated SIV challenge afforded by inactivated whole SIV vaccines.
abstract: This study attempted to determine if SIV vaccines could protect against challenge with peripheral blood mononuclear cells (PBMCs) from an SIV infected rhesus monkey. Mature Macaca mulatta were vaccinated four times with formalin inactivated SIVmac32H administered in MDP adjuvant (n = 8) or SIVmac32H ISCOM vaccine (n = 8). Controls included animals vaccinated with measles virus in MDP adjuvant (n = 4) or ISCOM (n = 4) preparations. Of each group, half were challenged intravenously (IV) with ten MID50 of the cell-free SIVmac32H (11-88) SIV stock and half were challenged with ten MID50 of PBMCs from the SIVmac32H infected macaque 1XC. All SIV vaccinated animals challenged with the 11-88 cell free stock of SIVmac32H were protected, whereas only half of the SIV vaccinated monkeys receiving the same infectious dose of the 1XC cell stock were protected.
authors.affiliation: Laboratory of Viral Pathogenesis, Institute of Applied Radiobiology and Immunology TNO, Rijswijk, The Netherlands.
authors.forename: J L
authors.initials: JL
authors.lastname: Heeney
authors.forename: P
authors.initials: P
authors.lastname: de Vries
authors.forename: R
authors.initials: R
authors.lastname: Dubbes
authors.forename: W
authors.initials: W
authors.lastname: Koornstra
authors.forename: H
authors.initials: H
authors.lastname: Niphuis
authors.forename: P
authors.initials: P
authors.lastname: ten Haaft
authors.forename: J
authors.initials: J
authors.lastname: Boes
authors.forename: M E
authors.initials: ME
authors.lastname: Dings
authors.forename: B
authors.initials: B
authors.lastname: Morein
authors.forename: A D
authors.initials: AD
authors.lastname: Osterhaus
mesh_terms: D000276:Adjuvants, Immunologic; D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D016882:ISCOMs; D007963:Leukocytes, Mononuclear / Q000382:microbiology*; D008253:Macaca mulatta; D016097:Simian Acquired Immunodeficiency Syndrome / Q000517:prevention & control*; D015302:Simian Immunodeficiency Virus / Q000276:immunology* / Q000302:isolation & purification; D014611:Vaccination / Q000662:veterinary; D015164:Vaccines, Inactivated / Q000276:immunology; D014765:Viral Vaccines / Q000276:immunology
chemical_list: D000276:Adjuvants, Immunologic; D000914:Antibodies, Viral; D016882:ISCOMs; D015164:Vaccines, Inactivated; D014765:Viral Vaccines