title: The ERE-like sequence from the promoter region of the testis specific hsp70-related gene is not estrogen responsive.
abstract: The 5'flanking region of the hsp70-related gene which is specifically expressed in rat and mouse spermatocytes contains the GGTCAnnnCGACC sequence closely resembling the palindromic consensus ERE (estrogen responsive element, GGTCAnnnTGACC). We used a transient expression assay to study whether this ERE-like sequence can transfer estrogen responsiveness either to heterologous or homologous promoters linked to a reporter CAT gene. We found that the GGTCAnnnCGACC sequence either as an isolated element or in its natural sequence context does not respond to hormone stimulation in MCF-7 and Fe33 cells. This observation points to the importance of the T-nucleotide in a right half-palindrome of ERE for ligand-dependent activation of transcription.
authors.affiliation: Department of Tumor Biology, Institute of Oncology, Gliwice, Poland.
authors.forename: Z
authors.initials: Z
authors.lastname: Krawczyk
authors.forename: W
authors.initials: W
authors.lastname: Schmid
authors.forename: P
authors.initials: P
authors.lastname: Harkonen
authors.forename: P
authors.initials: P
authors.lastname: Wolnirzek
mesh_terms: D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D004958:Estradiol / Q000494:pharmacology; D006360:Heat-Shock Proteins / Q000235:genetics*; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D051381:Rats; D013090:Spermatocytes / Q000187:drug effects; D015534:Trans-Activators / Q000235:genetics*; D014162:Transfection
chemical_list: D006360:Heat-Shock Proteins; D015534:Trans-Activators; D004958:Estradiol