Tissue-specific response of estrogen receptor gene expression to estrogen in chick.
We have developed a sensitive assay system by RT (reverse transcription)-PCR(polymerase chain reaction) to detect the low level of chicken estrogen receptor (cER) transcript. Using this system, the differential expression of cER gene in chick tissues was observed. Moreover, we found that the oviduct cER transcript levels in chick was not affected, albeit the remarkable growth of this tissue, by exogenous estrogen. In contrast, estrogen enhanced the hepatic cER transcript level several folds. Thus, the present study clearly showed a novel tissue-specific response of cER gene expression to estrogen in chick.
Ninomiya
Y
Y
Department of Agricultural Chemistry, Faculty of Agriculture, Tokyo University of Agriculture, Japan.
Mochii
M
M
Eguchi
G
G
Hasegawa
T
T
Masushige
S
S
Kato
S
S
D000818:Animals; D002645:Chickens; D004967:Estrogens / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects*; D008099:Liver / Q000502:physiology; D010057:Oviducts / Q000502:physiology; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics; D011960:Receptors, Estrogen / Q000235:genetics*
D004967:Estrogens; D012333:RNA, Messenger; D011960:Receptors, Estrogen