Localization of the photoreceptor gene ROM1 to human chromosome 11 and mouse chromosome 19: sublocalization to human 11q13 between PGA and PYGM.
Rom-1 is a retinal integral membrane protein that, together with the product of the human retinal degeneration slow gene (RDS), defines a photoreceptor-specific protein family. The gene for rom-1 (HGM symbol: ROM1) has been assigned to human chromosome 11 and mouse chromosome 19 by Southern blot analysis of somatic cell hybrid DNAs. ROM1 was regionally sublocalized to human 11p13-11q13 by using three mouse-human somatic cell hybrids; in situ hybridization refined the sublocalization to human 11q13. Analysis of somatic cell hybrids suggested that the most likely localization of ROM1 is in the approximately 2-cM interval between human PGA (human pepsinogen A) and PYGM (muscle glycogen phosphorylase). ROM1 appears to be a new member of a conserved syntenic group whose members include such genes as CD5, CD20, and OSBP (oxysterol-binding protein), on human chromosome 11 and mouse chromosome 19. Localization of the ROM1 gene will permit the examination of its linkage to hereditary retinopathies in man and mouse.
Bascom
R A
RA
Department of Genetics, Hospital for Sick Children, Toronto, Ontario, Canada.
García-Heras
J
J
Hsieh
C L
CL
Gerhard
D S
DS
Jones
C
C
Francke
U
U
Willard
H F
HF
Ledbetter
D H
DH
McInnes
R R
RR
D000818:Animals; D015139:Blotting, Southern; D002874:Chromosome Mapping; D002880:Chromosomes, Human, Pair 11; D005136:Eye Proteins / Q000235:genetics*; D005260:Female; D006801:Humans; D006822:Hybrid Cells; D008565:Membrane Proteins / Q000235:genetics*; D051379:Mice; D010435:Pepsinogens / Q000235:genetics*; D006005:Phosphorylases / Q000235:genetics*
D005136:Eye Proteins; D008565:Membrane Proteins; D010435:Pepsinogens; D006005:Phosphorylases