[Effects of nitroglycerin on intracellular concentration of calcium in human blood platelets].
It is shown that nitroglycerin has a calcium-blocking property in human platelets both at basal and ADP-induced calcium level, determined by fluorescence techniques (Fura-2). The study demonstrated a dose-dependent inhibitory effect of nitroglycerin on calcium rise, induced by ADP. 30-min preincubation with 60 microM nitroglycerin increased IC50 from 44 (without preincubation) to 320 microM, indicating the development of nitrate tolerance. It was suggested, that human platelet may be used as an experimental model of nitrate tolerance.
Shilo
V Iu
VIu
Storozhakov
G I
GI
Sergeev
P V
PV
Dukhanin
A S
AS
Trepilets
V E
VE
D000244:Adenosine Diphosphate / Q000494:pharmacology; D001792:Blood Platelets / Q000737:chemistry* / Q000187:drug effects; D002118:Calcium / Q000097:blood*; D004361:Drug Tolerance; D005453:Fluorescence; D006801:Humans; D008297:Male; D005996:Nitroglycerin / Q000494:pharmacology*
D000244:Adenosine Diphosphate; D005996:Nitroglycerin; D002118:Calcium
Vliianie nitroglitserina na kontsentratsiiu vnutrikletochnogo kal'tsiia v trombotsitakh cheloveka.