title: The nucleotide sequence of apple stem grooving capillovirus genome.
abstract: The complete nucleotide sequence of apple stem grooving virus (ASGV) genome has been determined. The genome is 6496 nucleotides in length excluding a 3'-terminal poly(A) tail and contains two overlapping open reading frames (ORFs). ORF1 begins at nucleotide position 37 and is terminated at position 6341, encoding a protein with a molecular weight of 241 kDa. ORF2, which is in a different reading frame within ORF1, begins at position 4788 and can encode a 36-kDa protein. The 241-kDa protein contains two consensus sequences associated with the RNA-dependent RNA polymerase and the NTP-binding helicase. Comparisons of amino acid sequences around these conserved motifs with other RNA viruses revealed that ASGV has extensive similarities with apple chlorotic leaf spot, tymo-, carla-, and potexviruses, and is a member of the sindbis-like supergroup. ASGV coat protein is found to be located in the C-terminal region of the 241-kDa polyprotein. The 36-kDa protein encoded by ORF2 contains the consensus sequence Gly-Asp-Ser-Gly found in the active site of several cellular and viral serine proteases.
authors.affiliation: Faculty of Agriculture, Iwate University, Morioka, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Yoshikawa
authors.forename: E
authors.initials: E
authors.lastname: Sasaki
authors.forename: M
authors.initials: M
authors.lastname: Kato
authors.forename: T
authors.initials: T
authors.lastname: Takahashi
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D005075:Biological Evolution; D015153:Blotting, Western; D002213:Capsid / Q000235:genetics; D002918:Chymotrypsin / Q000235:genetics; D004279:DNA, Viral; D005091:Exons; D016679:Genome, Viral; D008969:Molecular Sequence Data; D010942:Plant Viruses / Q000235:genetics*; D012319:RNA Polymerase II / Q000235:genetics; D012328:RNA Viruses / Q000235:genetics*; D012367:RNA, Viral; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D012697:Serine Endopeptidases / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins
chemical_list: D002918:Chymotrypsin; D004279:DNA, Viral; D012319:RNA Polymerase II; D012367:RNA, Viral; D012697:Serine Endopeptidases; D014764:Viral Proteins; C023302:yeast proteinase B; D029701:Saccharomyces cerevisiae Proteins