Mast-cell-derived mediators induce epidermal cell proliferation: clue for lichenified skin lesion formation in atopic dermatitis.
Mast-cell-derived mediators showed mitogenic activities on mouse-transformed epidermal cell line Pam 212 cells. These activities were eluted into the low-molecular-weight fractions below a molecular weight of 10 kD on a high performance liquid chromatography TSK 2000G column, and were partially abrogated by antihistamines or anticytokine antibodies, including anti-IL1 alpha, -IL1 beta or IL6 antibodies. Pretreatment of mast cell lines with sodium butyrate enhanced the production of these factors. Calcium ionophore or Concanavalin A (ConA) stimulated mast cells to generate factor production. These results suggest that mast-cell-derived mediators might play some role in epidermal hyperplasia seen in lichenified lesions in atopic dermatitis.
Katayama
I
I
Department of Dermatology, Tokyo Medical and Dental University, School of Medicine, Japan.
Yokozeki
H
H
Nishioka
K
K
D000818:Animals; D002455:Cell Division / Q000502:physiology; D002461:Cell Line, Transformed; D003876:Dermatitis, Atopic / Q000473:pathology*; D000078404:Epidermal Cells; D006133:Growth Substances / Q000096:biosynthesis / Q000302:isolation & purification / Q000502:physiology*; D017512:Lichenoid Eruptions / Q000473:pathology*; D008407:Mast Cells / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D014407:Tumor Cells, Cultured
D006133:Growth Substances