An S1 nuclease-sensitive homopurine/homopyrimidine domain in the PDGF A-chain promoter contains a novel binding site for the growth factor-inducible protein EGR-1.
Transcription of the platelet-derived growth factor (PDGF) A-chain gene is activated in cells exposed to growth factors. We now have identified a homopurine/homopyrimidine domain in the promoter region of the PDGF A-chain gene that exhibits S1 nuclease sensitivity in vitro and that contains a novel binding site (5'-TCCTCCTCCTCCTC-3') for the growth factor inducible transcription factor EGR-1 as demonstrated in gel mobility shift assays. Sequences similar to this novel EGR-1 binding site were observed also in five growth-related genes and shown to bind to EGR-1 in competition assays, suggesting that EGR-1 may influence the transcriptional regulation of these growth-related genes.
Wang
Z Y
ZY
Department of Medicine, Jewish Hospital, Washington University Medical Center, St. Louis, MO 63110.
Deuel
T F
TF
D001483:Base Sequence; D001665:Binding Sites; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000378:metabolism*; D006133:Growth Substances / Q000494:pharmacology; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D010982:Platelet-Derived Growth Factor / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012689:Sequence Homology, Nucleic Acid; D015719:Single-Strand Specific DNA and RNA Endonucleases / Q000378:metabolism*; D013379:Substrate Specificity; D014157:Transcription Factors / Q000096:biosynthesis / Q000378:metabolism*
D004268:DNA-Binding Proteins; D006133:Growth Substances; D046911:Macromolecular Substances; D009838:Oligodeoxyribonucleotides; D010982:Platelet-Derived Growth Factor; D014157:Transcription Factors; D015719:Single-Strand Specific DNA and RNA Endonucleases