title: Human focal cerebral ischemia: evaluation of brain pH and energy metabolism with P-31 NMR spectroscopy.
abstract: The authors investigated early human focal ischemia with phosphorus-31 nuclear magnetic resonance spectroscopy at 1.89 T to characterize the temporal evolution and relationship of brain pH and phosphate energy metabolism. Data from 65 symptomatic patients were prospectively studied; none of the patients had had ischemic stroke in the internal carotid artery territory before. Twenty-eight neurologically normal individuals served as control subjects. Serial ischemic brain pH levels indicated a progression from early acidosis to subacute alkalosis. When acidosis was present there was a significant elevation in the relative signal intensity of inorganic phosphate (Pi) and significant reductions in signal intensities of alpha-adenosine triphosphate (ATP) and gamma-ATP compared with those of control subjects. Ischemic brain pH values directly correlated with the relative signal intensity of phosphocreatine (PCr) and the PCr index and inversely correlated with the signal intensity of Pi. There was a general lack of correlation between either ischemic brain pH or phosphate energy metabolism and the initial clinical stroke severity. The data suggest a link between high-energy phosphate metabolism and brain pH, especially during the period of ischemic brain acidosis, and the authors propose that effective acute stroke therapy should be instituted during this period.
authors.affiliation: Department of Neurology, Henry Ford Hospital and Health Sciences Center, Detroit, MI 48202-2689.
authors.forename: S R
authors.initials: SR
authors.lastname: Levine
authors.forename: J A
authors.initials: JA
authors.lastname: Helpern
authors.forename: K M
authors.initials: KM
authors.lastname: Welch
authors.forename: A M
authors.initials: AM
authors.lastname: Vande Linde
authors.forename: K L
authors.initials: KL
authors.lastname: Sawaya
authors.forename: E E
authors.initials: EE
authors.lastname: Brown
authors.forename: N M
authors.initials: NM
authors.lastname: Ramadan
authors.forename: R K
authors.initials: RK
authors.lastname: Deveshwar
authors.forename: R J
authors.initials: RJ
authors.lastname: Ordidge
mesh_terms: D000138:Acidosis / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001921:Brain / Q000378:metabolism*; D002544:Cerebral Infarction / Q000378:metabolism; D002561:Cerebrovascular Disorders / Q000378:metabolism; D004734:Energy Metabolism; D005260:Female; D006801:Humans; D006863:Hydrogen-Ion Concentration; D002546:Ischemic Attack, Transient / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D008875:Middle Aged; D010710:Phosphates / Q000378:metabolism*; D010725:Phosphocreatine / Q000378:metabolism; D010758:Phosphorus
chemical_list: D010710:Phosphates; D010725:Phosphocreatine; D010758:Phosphorus; D000255:Adenosine Triphosphate