Arachidonic acid channelling in the phospholipid fractions and subcellular compartments of cultured myocardial cells.
Arachidonic acid (AA) channeling in cultured heart cells was studied following pulse labelling for 1 h. AA was shown to be esterified immediately and equally distributed between the neutral lipids and phospholipids. A rapid constant flow to various phospholipid classes occurred thereafter, while the AA oxidation was only between 12%. The subcellular distribution of AA was studied by nitrogen cavitation followed by fractionation on 6.7% percoll in sucrose-EDTA. After 1 h pulse labeling and 2 h post-pulse incubation, most of the radioactivity was found in the sarcolemmal fraction with a much smaller amount in the mitochondrial fraction.
Halabi
A
A
Laboratory for Myocardial Research, Hebrew University-Hadassah Medical School, Jerusalem, Israel.
Nachas
N
N
Pinson
A
A
D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism*; D002250:Carbon Radioisotopes; D002478:Cells, Cultured; D009206:Myocardium / Q000737:chemistry / Q000166:cytology / Q000378:metabolism*; D010743:Phospholipids / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D013347:Subcellular Fractions / Q000378:metabolism
D002250:Carbon Radioisotopes; D010743:Phospholipids; D016718:Arachidonic Acid