New approaches to assess topical corticosteroid bioequivalence: pharmacokinetic evaluation.
An ideal method for measuring the bioavailability of topical corticosteroids should be simple, accurate, and adaptable to a variety of settings and should not require extensive special training to perform. Drug uptake into the stratum corneum, measured by tapestripping, is correlated with the pharmacodynamic response of skin blanching, observed in the vasoconstrictor assay. Differences in stratum corneum drug uptake can be objectively quantitated as a function of time, occlusion, dose applied, and vehicle. Tapestripping measurements are reproducible within individual subjects, but large interindividual variabilities may exist. The chromameter, a new technology, objectively quantitates color numerically and can be used to measure skin blanching as part of the pharmacodynamic response to topical corticosteroids. The chromameter offers an easy, objective method with which to quantitate the pharmacodynamic response of topical corticosteroids. Both methods allow a more mechanistic approach than currently used methods to investigate topical drug pharmacokinetics and pharmacodynamics.
Pershing
L K
LK
Division of Dermatology, University of Utah Health Sciences Center, Salt Lake City 84132.
D000818:Animals; D001623:Betamethasone / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics* / Q000494:pharmacology; D046508:Culture Techniques; D015194:Diffusion Chambers, Culture; D004334:Drug Administration Schedule; D004337:Drug Carriers; D004341:Drug Evaluation; D004353:Drug Evaluation, Preclinical; D006801:Humans; D008567:Membranes, Artificial; D051379:Mice; D008819:Mice, Nude; D014677:Pharmaceutical Vehicles; D012867:Skin / Q000187:drug effects / Q000378:metabolism; D016038:Skin Transplantation; D012995:Solubility; D013810:Therapeutic Equivalency; D014183:Transplantation, Heterologous; D014662:Vasoconstrictor Agents / Q000494:pharmacology
D004337:Drug Carriers; D008567:Membranes, Artificial; D014677:Pharmaceutical Vehicles; D014662:Vasoconstrictor Agents; D001623:Betamethasone