title: Schistosoma mansoni: control of hepatotoxicity and egg excretion by immune serum in infected immunosuppressed mice is schistosome species-specific, but not S. mansoni strain-specific.
abstract: Immunosuppressed mice infected with Schistosoma mansoni suffer from an acute hepatotoxicity reaction, and they fail to excrete as many parasite eggs as comparably infected immunologically intact control animals. The hepatotoxicity was shown here to be preventable, and egg excretion rates were enhanced, by transfer of serum from donors with chronic S. mansoni infections, but not by serum from donors with heterologous infections of Schistosoma haematobium, Schistosoma bovis, or Schistosoma japonicum. The effects of the transferred sera are considered to be due to specific antibody, but the possibility of cytokine involvement is discussed. A high degree of serological cross-reactivity was found between sera from mice infected with the different schistosome species and unfractionated egg homogenate (SEA) in ELISA. Cross-reactivity of the heterologous sera was, however, reduced against CEF6, a partially purified fraction of S. mansoni eggs that contains the putative hepatotoxin and has serodiagnostic potential. S. mansoni isolates from Puerto Rico, Brazil, Egypt, and Kenya shared similar characteristics with respect to the immune dependence of egg excretion and hepatotoxicity in immunosuppressed mice. The S. mansoni geographic isolates were also indistinguishable serologically, in terms of both the capacity of respective infection sera to neutralize hepatotoxicity and in their capacity to promote egg excretion of the other isolates in vivo. Complete immunological cross-reactivity of the geographically distinct isolates was also observed in ELISA with both CEF6 and SEA. Utilization of CEF6 for serodiagnosis of schistosomiasis mansoni is therefore unlikely to be restricted by geographical considerations.
authors.affiliation: School of Biological Sciences, University College of North Wales, Bangor, Gwynedd, U.K.
authors.forename: H M
authors.initials: HM
authors.lastname: Murare
authors.forename: D W
authors.initials: DW
authors.lastname: Dunne
authors.forename: J
authors.initials: J
authors.lastname: Bain
authors.forename: M J
authors.initials: MJ
authors.lastname: Doenhoff
mesh_terms: D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology*; D003429:Cross Reactions; D004527:Egg Proteins / Q000276:immunology*; D014644:Genetic Variation; D007106:Immune Sera / Q000276:immunology*; D007165:Immunosuppression Therapy; D016219:Immunotherapy, Adoptive; D008099:Liver / Q000469:parasitology / Q000473:pathology*; D051379:Mice; D008808:Mice, Inbred CBA; D010063:Ovum / Q000276:immunology*; D012555:Schistosomiasis mansoni / Q000276:immunology*; D013045:Species Specificity
chemical_list: D000947:Antigens, Helminth; D004527:Egg Proteins; D007106:Immune Sera