DNA strand cleavage at 8-hydroxyguanine residues by hot piperidine treatment.
The two findings indicate that 8-hydroxyguanine(8-OH-Gua) is a hot piperidine-sensitive lesion in DNA. These are cleavages of DNA containing 8-OH-Gua at the site of this residue and decomposition of 8-hydroxydeoxyguanosine when the DNA and nucleoside were treated in 1 M piperidine for 30 min at 90 degrees C. However, no cleavage was observed in DNA containing 8-hydroxyadenine or O6-methylguanine. 8-OH-Gua was found to be different from apurinic sites that are also alkali-labile lesions since the former was more resistant to alkali treatment. This property of 8-OH-Gua can be used as a check for the incorporation of this base into DNA after the synthesis of DNA containing 8-OH-Gua at a specific position or possibly can be one of the markers for the identification of 8-OH-Gua formed in DNA exposed to reactive oxygen species.
Chung
M H
MH
Department of Pharmacology, Seoul National University, College of Medicine, Korea.
Kiyosawa
H
H
Ohtsuka
E
E
Nishimura
S
S
Kasai
H
H
D001483:Base Sequence; D004247:DNA / Q000737:chemistry* / Q000187:drug effects; D004249:DNA Damage; D006147:Guanine / Q000031:analogs & derivatives*; D006358:Hot Temperature; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis / Q000737:chemistry*; D010880:Piperidines / Q000494:pharmacology; D013056:Spectrophotometry, Ultraviolet
D009838:Oligodeoxyribonucleotides; D010880:Piperidines; C024829:8-hydroxyguanine; D006147:Guanine; C032727:piperidine; D004247:DNA