title: Conformation and elasticity of the isolated red blood cell membrane skeleton.
abstract: We studied the structure and elasticity of membrane skeletons from human red blood cells (RBCs) during and after extraction of RBC ghosts with nonionic detergent. Optical tweezers were used to suspend individual cells inside a flow chamber, away from all surfaces; this procedure allowed complete exchange of medium while the low-contrast protein network of the skeleton was observed by high resolution, video-enhanced differential interference-contrast (DIC) microscopy. Immediately following extraction in a 5 mM salt buffer, skeletons assumed expanded, nearly spherical shapes that were uncorrelated with the shapes of their parent RBCs. Judging by the extent of thermal undulations and by their deformability in small flow fields, the bending rigidity of skeletons was markedly lower than that of either RBCs or ghosts. No further changes were apparent in skeletons maintained in this buffer for up to 40 min at low temperatures (T less than 10 degrees C), but skeletons shrank when the ionic strength of the buffer was increased. When the salt concentration was raised to 1.5 M, shrinkage remained reversible for approximately 1 min but thereafter became irreversible. When maintained in 1.5 M salt buffer for longer periods, skeletons continued to shrink, lost flexibility, and assumed irregular shapes: this rigidification was irreversible. At this stage, skeletons closely resembled those isolated in standard bulk preparations. We propose that the transformation to the rigid, irreversibly shrunken state is a consequence of spectrin dimer-dimer reconnections and that these structural rearrangements are thermally activated. We also measured the salt-dependent size of fresh and bulk extracted skeletons. Our measurements suggest that, in situ, the spectrin tethers are flexible, with a persistence length of approximately 10 nm at 150 mM salt.
authors.affiliation: Department of Physics, Harvard University, Cambridge, Massachusetts 02138.
authors.forename: K
authors.initials: K
authors.lastname: Svoboda
authors.forename: C F
authors.initials: CF
authors.lastname: Schmidt
authors.forename: D
authors.initials: D
authors.lastname: Branton
authors.forename: S M
authors.initials: SM
authors.lastname: Block
mesh_terms: D004548:Elasticity; D004910:Erythrocyte Membrane / Q000648:ultrastructure*; D006801:Humans; D008297:Male; D008857:Microscopy, Interference / Q000295:instrumentation / Q000379:methods; D009994:Osmolar Concentration; D014741:Video Recording / Q000295:instrumentation / Q000379:methods