Development of a transformation system for the thermophilic fungus Talaromyces sp. CL240 based on the use of phleomycin resistance as a dominant selectable marker.
A transformation system for the thermophilic cellulolytic fungus Talaromyces sp. CL240 has been developed, using the phleomycin resistance gene from Streptoalloteichus hindustanus (Sh ble) as a dominant selectable marker. The plasmids (pAN8-1 and pUT720) carrying the Sh ble gene under the control of the Aspergillus nidulans glyceraldehyde-3-phosphate dehydrogenase (gpd) promoter, allowed selection of phleomycin-resistant transformants. A new promoter sequence cloned from chromosomal DNA of Trichoderma reesei (pUT737) was also able to drive efficient expression of the Sh ble gene in Talaromyces sp. CL240, resulting in the selection of transformants that were highly resistant to phleomycin.
Jain
S
S
Laboratoire de Microbiologie et Génétique Appliqués du C.N.R.S., C.R.B.G.C., Université Paul Sabatier, Toulouse, France.
Durand
H
H
Tiraby
G
G
D004352:Drug Resistance, Microbial; D005658:Fungi / Q000235:genetics*; D015966:Gene Expression Regulation, Fungal; D005822:Genetic Vectors; D006358:Hot Temperature; D010692:Phleomycins / Q000494:pharmacology*; D010957:Plasmids; D011401:Promoter Regions, Genetic; D014170:Transformation, Genetic
D010692:Phleomycins