Development of concanavalin A-enzyme immunosorbent assay for glycated haptoglobin using polyclonal and monoclonal antibodies.
Two-site lectin-haptoglobin-enzyme immunosorbent assay (L-Hp-ELISA), is described. Haptoglobin binding to Concanavalin A, immobilized to polystyrene microtiter plate, was estimated by anti-haptoglobin polyclonal and monoclonal antibodies conjugated with horse-radish peroxidase. The range of haptoglobin binding to Concanavalin A, measured by the L-Hp-ELISA was 25 to 300 ng/ml using polyclonal, and 50 to 600 ng/ml using monoclonal anti-haptoglobin antibodies, respectively.
Katnik
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Department of Biochemistry, Medical Academy, Wrocław, Poland.
Dobryszycka
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D000107:Acetylation; D000906:Antibodies; D000911:Antibodies, Monoclonal; D001665:Binding Sites; D003208:Concanavalin A; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005069:Evaluation Studies as Topic; D006031:Glycosylation; D006242:Haptoglobins / Q000032:analysis* / Q000737:chemistry / Q000276:immunology; D006801:Humans; D012680:Sensitivity and Specificity
D000906:Antibodies; D000911:Antibodies, Monoclonal; D006242:Haptoglobins; D003208:Concanavalin A