title: Lymphocyte and lymphocyte subset numbers in blood and in bronchoalveolar lavage and pleural fluid in various forms of human pulmonary tuberculosis at presentation and during recovery.
abstract: BACKGROUND
  Lymphocytes have a central role in human defences against mycobacteria. A study was designed to assess the relation between lymphocyte responses and clinical pattern of disease, nutrition and recovery during treatment in patients with tuberculosis.
  
  
  METHODS
  Lymphocyte numbers and subsets (on the basis of CD3, CD4, and CD8 monoclonal antibodies) were measured in peripheral blood and, where appropriate, bronchoalveolar lavage or pleural fluid of patients with different forms of pulmonary tuberculosis. Eleven had localised pulmonary tuberculosis, 18 miliary tuberculosis and seven a tuberculous pleural effusion.
  
  
  RESULTS
  CD4 lymphocytes were found in greatly increased numbers in pleural fluid and were relatively depleted in the blood. Lymphocyte numbers in bronchoalveolar lavage fluid varied widely in localised pulmonary and miliary tuberculosis but were highest in lavage fluid from patients with miliary tuberculosis. This was due to an increase in CD8 lymphocytes, which were also increased in the blood. Lymphocyte numbers bore no relation to nutrition, symptom duration, or radiographic profusion scores. In miliary tuberculosis the time taken for the chest radiograph to clear (mean (SD) 17.6 (7.8) weeks) correlated with lymphocyte numbers in lavage fluid, especially CD8 cells (r = 0.74), but not with the patients' age or nutrition. After 8 weeks' treatment, total and CD4 lymphocyte numbers in lavage fluid showed a substantial increase.
  
  
  CONCLUSIONS
  The association of CD8 cells with delayed recovery is compatible with suppression of the antimycobacterial action of macrophages. The switch to predominance of CD4 cells in lavage fluid during successful treatment supports the view that they may have a role in eliminating mycobacteria.
authors.affiliation: Department of Medicine, University of Cape Town, South Africa.
authors.forename: G M
authors.initials: GM
authors.lastname: Ainslie
authors.forename: J A
authors.initials: JA
authors.lastname: Solomon
authors.forename: E D
authors.initials: ED
authors.lastname: Bateman
mesh_terms: D000328:Adult; D001992:Bronchoalveolar Lavage Fluid / Q000473:pathology*; D005260:Female; D006801:Humans; D007958:Leukocyte Count; D016131:Lymphocyte Subsets / Q000473:pathology*; D008214:Lymphocytes / Q000473:pathology; D008297:Male; D010996:Pleural Effusion / Q000473:pathology*; D016176:T-Lymphocyte Subsets / Q000473:pathology; D013997:Time Factors; D014397:Tuberculosis, Pulmonary / Q000188:drug therapy / Q000473:pathology*