title: [Development and use of a method of determination of specific immunoglobulin G based on immunoenzyme analysis in the diagnosis of pulmonary aspergillosis].
abstract: Enzyme-linked immunoassay (ELISA) was employed for determination of specific immunoglobulin G to Aspergillus fumigatus (A.f.). Culture filtrate of A.f. was used as antigen. Optimal conditions of ELISA were found. Serum of 138 asthmatic patients with total IgE level more than 500 PRU/ml were analysed for determining the limits of IgG to A.f. concentrations.
authors.forename: S Iu
authors.initials: SIu
authors.lastname: Vlasenko
authors.forename: Iu S
authors.initials: IuS
authors.lastname: Lebedin
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000908:Antibodies, Fungal / Q000032:analysis*; D001228:Aspergillosis / Q000175:diagnosis* / Q000276:immunology; D001232:Aspergillus fumigatus / Q000276:immunology*; D001249:Asthma / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis*; D008172:Lung Diseases, Fungal / Q000175:diagnosis* / Q000276:immunology; D008297:Male; D008875:Middle Aged
chemical_list: D000908:Antibodies, Fungal; D007074:Immunoglobulin G
vernacular_title: Razrabotka i primenenie metoda opredeleniia spetsificheskogo immunoglobulina G na osnove immunofermentnogo analiza dlia diagnostiki aspergilleza legkikh.