Absence of measles, mumps, and rubella viral genomic sequences from multiple sclerosis brain tissue by polymerase chain reaction.
We tested for measles, mumps, and rubella viruses in multiple sclerosis by polymerase chain reaction (PCR). Using RNA extracted from 19 multiple sclerosis and 8 control brain specimens, nested PCR was performed after reverse transcription (RT) of the RNA to cDNA using primer pairs directed against two regions in the genomes of measles and mumps viruses and one region in the rubella virus genome. Despite enhanced sensitivity of nested RT PCR, measles, mumps, and rubella viral genomic sequences were not found in any brain specimen.
Godec
M S
MS
Laboratory of Central Nervous System Studies, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892.
Asher
D M
DM
Murray
R S
RS
Shin
M L
ML
Greenham
L W
LW
Gibbs
C J
CJ
Gajdusek
D C
DC
D000328:Adult; D000368:Aged; D001483:Base Sequence; D001921:Brain / Q000382:microbiology*; D005260:Female; D006801:Humans; D008297:Male; D008459:Measles virus / Q000235:genetics / Q000302:isolation & purification*; D008875:Middle Aged; D008969:Molecular Sequence Data; D009103:Multiple Sclerosis / Q000382:microbiology*; D009109:Mumps virus / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods; D012367:RNA, Viral / Q000302:isolation & purification*; D012412:Rubella virus / Q000235:genetics / Q000302:isolation & purification*; D012680:Sensitivity and Specificity
D012367:RNA, Viral