[p26--a calcium binding protein from photoreceptor cells in the bovine retina: primary structure and expression in E. coli].
The primary structure of the bovine retinal calcium binding protein P26 has been determined by the parallel analysis of the protein and the corresponding cDNA. This protein is identical to recovering and shares 59% homology with visinin, a cone specific calcium binding protein from chicken retina. P26 was expressed in E. coli as a fusion protein and, after purification by affinity chromatography on IgG-Sepharose 6, cleaved off with enteropeptidase.
Kutuzov
M A
MA
Shmukler
B E
BE
Suslov
O N
ON
Zargarov
A A
AA
Abdulaev
N G
NG
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002135:Calcium-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D002417:Cattle; D004247:DNA; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics*; D005136:Eye Proteins / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D007908:Lens, Crystalline / Q000378:metabolism*; D008969:Molecular Sequence Data; D010786:Photoreceptor Cells / Q000378:metabolism*; D010957:Plasmids; D051603:Recoverin; D013329:Structure-Activity Relationship
D002135:Calcium-Binding Proteins; D005136:Eye Proteins; C067574:RCV1 protein, Bos taurus; D051603:Recoverin; D004247:DNA
p26--kal'tsiĭsviazyvaiushchiĭ belok fotoretseptornykh kletok setchatki byka: pervichnaia struktura i ékspressiia v E. coli.