Establishment of hybridoma cells with natural killer(NK)-like activity against syngenic tumor cells.
The 4D1D4 hybridoma cells were derived from the fusion of spleen cells from BALB/c nude mice with NS-1 mouse myeloma cells. The surface phenotypes of 4D1D4 hybridoma cells were Thy-1.2+, L3T4 (CD4)-, Lyt-2 (CD8)-, Asialo GM1+ and p-55 interleukin-2 (IL-2) receptor (CD25)-. This phenotypic pattern was consistent with the surface phenotype of NK cells. The 4D1D4 cells showed the definite killer activity against a syngenic tumor cell line, RL male-1, but not against an allogenic YAC-1 line. The killer activity of the 4D1D4 cells was not affected by the addition of exogenous IL-2. It was, therefore, suggested that 4D1D4 cells might be representative of resting NK cells with expression of no functional IL-2 receptors. The hybridoma technology might be useful for establishment of the cloned NK cells.
Suzuki
Y
Y
Laboratory of Bacteriology, Aichi Prefectural Institute of Public Health, Japan.
Funabashi
M
M
Isomura
S
S
Yoshikawa
K
K
Notake
K
K
Yokochi
T
T
D000818:Animals; D000943:Antigens, Differentiation / Q000032:analysis; D003602:Cytotoxicity, Immunologic; D006825:Hybridomas / Q000276:immunology*; D007376:Interleukin-2 / Q000494:pharmacology; D007694:Killer Cells, Natural / Q000187:drug effects / Q000276:immunology*; D051379:Mice; D014407:Tumor Cells, Cultured
D000943:Antigens, Differentiation; D007376:Interleukin-2