Repair of UV-induced (6-4)photoproducts measured in individual genes in the Drosophila embryonic Kc cell line.
The nucleotide excision repair (NER; dark-repair) of (6-4)photoproducts ((6-4)PPs) was assayed in cells from a permanent Drosophila melanogaster embryonic cell line, Kc, after exposure to 20 or 40 J/m2 ultraviolet (UV) light. Induction rates in the transcriptionally active genes Gart and Notch as well as in the inactive white locus is similar. They are formed with a frequency of about one-third of that of cyclobutane pyrimidine dimers (CPDs). In all three genes, (6-4)PPs are repaired with the same rate and to the same extent: 31% of the (6-4)PPs are removed in 4 hours post-irradiation and after 16 hours repair is nearly complete. In none of the three genes strand-specific repair was found. Exposure of cells that were irradiated with 40 J/m2 UV to photoreactivating light for 1 hour prior to dark-repair incubation, resulted in enhanced repair of (6-4)PPs.
de Cock
J G
JG
MGC-Department of Radiation Genetics and Chemical Mutagenesis, State University of Leiden, The Netherlands.
van Hoffen
A
A
Wijnands
J
J
Molenaar
G
G
Lohman
P H
PH
Eeken
J C
JC
D000818:Animals; D002460:Cell Line; D004247:DNA / Q000528:radiation effects*; D004249:DNA Damage; D004260:DNA Repair; D043224:Deoxyribonuclease (Pyrimidine Dimer); D004331:Drosophila melanogaster; D004625:Embryo, Nonmammalian; D004706:Endodeoxyribonucleases; D029968:Escherichia coli Proteins; D017344:Genes, Insect / Q000528:radiation effects*; D011740:Pyrimidine Dimers; D015183:Restriction Mapping; D014466:Ultraviolet Rays
D029968:Escherichia coli Proteins; D011740:Pyrimidine Dimers; D004247:DNA; D004706:Endodeoxyribonucleases; C036385:endodeoxyribonuclease uvrABC; D043224:Deoxyribonuclease (Pyrimidine Dimer)