[The expression of TGF beta-related mRNAs in the early development of Xenopus].
Using porcine TGF beta 1-cDNA probe, we found that three TGF beta-related mRNAs (4.2 kb, 3.2 kb and 2.3 kb) were detected in blastula (Stg. 7/8). The 4.2 kb and 3.2 kb mRNAs were very highly expressed in the blastula (Fig. 3). However, we could hardly detect them in embryos of cleavage, gestrula and neurula stages. On the other hand, the 2.3 kb mRNA could be identified in embryos of cleavage stages, blastula, gastrula and neurula and the quantity was rather stable. Comparing the quantities of TGF beta-related mRNAs in different parts of blastula, that is, animal half or vegetal half and dorsal or ventral half, we found that the transcripts were enriched in the vegetal hemisphere (Fig. 6). But no obvious difference between dorsal and ventral halves could be detected (Fig. 7).
Shou
W N
WN
Shanghai Institute of Cell Biology, Academia Sinica.
Song
Q B
QB
Chen
Y D
YD
Qian
R L
RL
D000818:Animals; D015342:DNA Probes; D004625:Embryo, Nonmammalian / Q000737:chemistry; D004627:Embryonic Induction; D012333:RNA, Messenger / Q000737:chemistry*; D016211:Transforming Growth Factor alpha / Q000235:genetics*; D014982:Xenopus laevis / Q000196:embryology* / Q000235:genetics
D015342:DNA Probes; D012333:RNA, Messenger; D016211:Transforming Growth Factor alpha