Use of magnetic resonance imaging to analyze the performance of hollow-fiber bioreactors.
Preliminary experiments were described that demonstrate that MRI is an effective tool for the noninvasive study of hollow-fiber bioreactors. Flow-compensated velocity-encoding pulse sequences were successively applied to analyze the velocity patterns in a module operated without cells, with an artificially induced flow field perturbation. Diffusion damping pulse sequences were also used to spatially resolve regions of cell growth in a bioreactor. These experiments provide the necessary basis from which future flow and spectroscopic studies can be conducted.
Donoghue
C
C
Department of Chemical Engineering, Worcester Polytechnic Institute, Massachusetts 01609.
Brideau
M
M
Newcomer
P
P
Pangrle
B
B
DiBiasio
D
D
Walsh
E
E
Moore
S
S
D000818:Animals; D001709:Biotechnology / Q000295:instrumentation / Q000379:methods; D002455:Cell Division; D002478:Cells, Cultured; D046508:Culture Techniques / Q000295:instrumentation* / Q000379:methods; D004058:Diffusion; D008279:Magnetic Resonance Imaging / Q000379:methods