In vitro study on the increase of trypsin resistance in the rat testicular sperm head.
The alteration of trypsin resistance in rat sperm head was investigated after the sperm were incubated in the culture media (199-Earele, 1% BSA) containing the inhibitors of protein synthesis. The head of non-incubated testicular sperm was easily digested by trypsin (1 mg/ml) while the head of the incubated sperm showed resistance to trypsin digestion after 3 hr-incubation. Trypsin resistance in epididymal sperm head did not change after the sperm was incubated. Neither cycloheximide (100 micrograms/ml) nor chloramphenicol (100 micrograms/ml) affected the trypsin resistance in the testicular sperm head.
Matsuzawa
T
T
Laboratory of Biology, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan.
Hoshino
T
T
Ibuki
J
J
D000818:Animals; D008297:Male; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D011506:Proteins / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013077:Sperm Head / Q000378:metabolism*; D013079:Sperm Maturation / Q000502:physiology; D013737:Testis / Q000166:cytology; D014357:Trypsin / Q000378:metabolism*
D011500:Protein Synthesis Inhibitors; D011506:Proteins; D014357:Trypsin