Detection of the heteromorphic spectrum of heterochromatin in the human genome by in situ digestion using restriction endonuclease AluI.
A battery of selective banding techniques has been utilized to identify the heteromorphic markers in the human genome. The recent addition of the AluI/Giemsa (G)-technique has helped not only in identifying the variable sites, but in characterizing their heteromorphic spectra. In the present investigation, we classified the pericentromeric heterochromatin by the AluI/G-technique by its size and position using 50 normal individuals and suggested the potential uses of this banding technique over earlier methods.
Luke
S
S
Division of Genetics, Long Island College Hospital-SUNY Health Science Center, Brooklyn 11201.
Verma
R S
RS
D002871:Chromosome Banding / Q000379:methods*; D004247:DNA / Q000235:genetics; D015252:Deoxyribonucleases, Type II Site-Specific; D005819:Genetic Markers; D015894:Genome, Human; D006570:Heterochromatin / Q000648:ultrastructure*; D006801:Humans
D005819:Genetic Markers; D006570:Heterochromatin; D004247:DNA; C024669:endodeoxyribonuclease AluI; D015252:Deoxyribonucleases, Type II Site-Specific