title: Fusion of phospholipid vesicles induced by phospholipase D in the presence of calcium ion.
abstract: Fusion of phospholipid vesicles composed of various combination of phosphatidylcholine, phosphatidylethanolamine and phosphatidic acid was induced by Ca2+ in the presence or absence of phospholipase D from Streptomyces chromofuscus. For the vesicles composed of 20:50:30 molar ratio of phosphatidylcholine/phosphatidylethanolamine/phosphatidic acid, the initial fusion rate was much faster than the expected value when only the conversion of phosphatidylcholine and phosphatidylethanolamine into phosphatidic acid by phospholipase D is taken into account. These observations are discussed in terms of the involvement of outer monolayer of the vesicles and the enzyme activity itself in the fusion process.
authors.affiliation: Department of Life Science, Korea Advanced Institute of Science and Technology, Yuseong-Gu, Taejon.
authors.forename: J B
authors.initials: JB
authors.lastname: Park
authors.forename: T H
authors.initials: TH
authors.lastname: Lee
authors.forename: H
authors.initials: H
authors.lastname: Kim
mesh_terms: D002118:Calcium / Q000494:pharmacology*; D004357:Drug Synergism; D008081:Liposomes; D008561:Membrane Fusion / Q000187:drug effects*; D010712:Phosphatidic Acids / Q000378:metabolism; D010713:Phosphatidylcholines / Q000378:metabolism; D010714:Phosphatidylethanolamines / Q000378:metabolism; D010739:Phospholipase D / Q000494:pharmacology*
chemical_list: D008081:Liposomes; D010712:Phosphatidic Acids; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D010739:Phospholipase D; D002118:Calcium