New analogues of Cucurbita maxima trypsin inhibitor III (CMTI III) with simplified structure.
Seven new analogues of trypsin inhibitor CMTI III were obtained by solid-phase peptide synthesis. Three analogues contained only two, instead of three, disulfide bridges, whereas the molecules of the next four analogues were shortened at the N- and/or C-terminus. The elimination of one disulfide bridge in CMTI III induces a decrease in the association equilibrium constants by 6-7 orders of magnitude, whereas the removal of one, two or three amino-acid residues at the N- and/or C-terminus does not significantly affect the activity.
Rolka
K
K
Faculty of Chemistry, University of Gdańsk, Poland.
Kupryszewski
G
G
Rózycki
J
J
Ragnarsson
U
U
Zbyryt
T
T
Otlewski
J
J
D000595:Amino Acid Sequence; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D014361:Trypsin Inhibitors / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology
C081870:CMTI protein, Cucurbita maxima; D010940:Plant Proteins; D014361:Trypsin Inhibitors