Multivariate statistical analysis of electron micrographs of a mammalian transcription initiation complex.
We describe the appearance of the murine ribosomal protein (rp) L32 gene transcription initiation complex as determined by electron image analysis. Using a fractionated nuclear extract enriched for transcription factors necessary for rpL32 gene transcription in vitro and a DNA fragment containing the rpL32 gene promoter, the transcription initiation complex was prepared and viewed by standard transmission electron microscopy. Image analysis demonstrated that the complex was a multilobed structure.
Harauz
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G
Department of Molecular Biology and Genetics, University of Guelph, Ontario, Canada.
Welsh
E A
EA
D002478:Cells, Cultured; D007091:Image Processing, Computer-Assisted; D046911:Macromolecular Substances; D008854:Microscopy, Electron; D012269:Ribosomal Proteins / Q000235:genetics* / Q000648:ultrastructure*; D014158:Transcription, Genetic
D046911:Macromolecular Substances; D012269:Ribosomal Proteins