[Establishment of immunofluorescence for the testing serum antibodies against inner ear tissues and its clinical application].
A modified Sainte-Marie technique for processing the inner ear tissues of guinea pigs was described in this paper. The inner ears were fixed over night in 95% cold ethanol, decalcified 7-10 days in 10% EDTA-Na2 at 4 degrees C, and embedded in paraffin. The deparaffinized sections were exposed 15 min in 95% ethanol precooled to 4 degrees C to fix the serum antibodies of patients and labeled antibody respectively. The sections were counterstained with Evans-blue. This method is simple, rapid, and sensitive, and antigenicity. Also, the specificity was enhanced and the preservation of fluorescence was prolonged. By means of this method, antibodies against inner ear tissue were found in the serum of 18 out of 30 patients with Meniere's disease, sudden deafness or uncertain forms of progressive sensorineural hearing loss. The results suggest that autoimmune response may play an important role in some inner ear disease.
Gong
S
S
Union hospital, Tongji Medical University, Wuhan.
D000293:Adolescent; D000328:Adult; D000818:Animals; D000936:Antigen-Antibody Complex / Q000097:blood; D001323:Autoantibodies / Q000032:analysis*; D003051:Cochlea / Q000276:immunology*; D005260:Female; D005455:Fluorescent Antibody Technique; D006168:Guinea Pigs; D006319:Hearing Loss, Sensorineural / Q000276:immunology; D003639:Hearing Loss, Sudden; D006801:Humans; D008297:Male; D008575:Meniere Disease / Q000276:immunology*; D008875:Middle Aged
D000936:Antigen-Antibody Complex; D001323:Autoantibodies