title: Long-term supplementation with n-3 fatty acids, II: Effect on neutrophil and monocyte chemotaxis.
abstract: The effect of a daily supplement with 4 g of n-3 polyunsaturated fatty acids (PUFA) for 9 months to 24 healthy volunteers on neutrophil and monocyte chemotaxis was studied using the under-agarose technique. Autologous serum and n-formyl-methionyl-leucyl-phenylalanine were used as chemoattractants. The effect after 9 months of supplementation with n-3 PUFA was also compared to results after short-term supplementation with n-3 PUFA for 6 weeks. Monocyte chemotaxis was reduced after 9 months of supplementation with n-3 PUFA to the same extent as after 6 weeks supplement. Neutrophil-directed migration towards chemoattractants was reduced after 9 months on fish oil, and this decrease was significantly greater than the decrease obtained after 6 weeks of supplementation. The spontaneous migration of neutrophils was significantly attenuated after 9 months compared to baseline and to 6 weeks. These findings lend support to a role for n-3 PUFA in the management of chronic inflammatory and atherosclerotic vascular diseases.
authors.affiliation: Department of Clinical Chemistry, Aalborg Hospital, Denmark.
authors.forename: E B
authors.initials: EB
authors.lastname: Schmidt
authors.forename: K
authors.initials: K
authors.lastname: Varming
authors.forename: J O
authors.initials: JO
authors.lastname: Pedersen
authors.forename: H H
authors.initials: HH
authors.lastname: Lervang
authors.forename: N
authors.initials: N
authors.lastname: Grunnet
authors.forename: C
authors.initials: C
authors.lastname: Jersild
authors.forename: J
authors.initials: J
authors.lastname: Dyerberg
mesh_terms: D000328:Adult; D002465:Cell Movement / Q000502:physiology; D002469:Cell Separation; D002634:Chemotaxis, Leukocyte / Q000502:physiology*; D004041:Dietary Fats / Q000008:administration & dosage*; D015525:Fatty Acids, Omega-3; D005231:Fatty Acids, Unsaturated / Q000008:administration & dosage*; D005260:Female; D005395:Fish Oils / Q000008:administration & dosage; D006801:Humans; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000502:physiology; D009504:Neutrophils / Q000502:physiology; D012016:Reference Values; D015203:Reproducibility of Results; D013997:Time Factors; D014280:Triglycerides / Q000008:administration & dosage
chemical_list: D004041:Dietary Fats; D015525:Fatty Acids, Omega-3; D005231:Fatty Acids, Unsaturated; D005395:Fish Oils; D014280:Triglycerides; C077446:Pikasol