title: Pathogenicity differences of multiple isolates of Aspergillus fumigatus in turkeys.
abstract: Sixteen Aspergillus fumigatus isolates of environmental, mammalian, and avian origin were used to assess: 1) intra-air-sac inoculation as a viable challenge alternative to aerosol exposure, and 2) isolate variability in pathogenicity. Development of lesions, antibody response in survivors, mortality, and weight gains were assessed. Turkey poults were challenged with equal numbers of viable conidia. Total number of conidia given per experimental group varied markedly and did not influence mortality. Antibody response as measured by the enzyme-linked immunosorbent assay and agar gel immunodiffusion test was erratic, although most poults with high antibody scores had marked lesions and low weight. Lesions were characterized by necrogranulomatous pneumonia and airsacculitis with marked visceral involvement. The source of the isolate was not a factor in mortality, although this was biased by the high numbers of isolates from birds with aspergillosis. The single environmental isolate produced no mortality.
authors.affiliation: U.S. Department of Agriculture, National Animal Disease Center, Ames, Iowa 50010.
authors.forename: W M
authors.initials: WM
authors.lastname: Peden
authors.forename: K R
authors.initials: KR
authors.lastname: Rhoades
mesh_terms: D000818:Animals; D000908:Antibodies, Fungal / Q000276:immunology; D001228:Aspergillosis / Q000401:mortality / Q000662:veterinary*; D001232:Aspergillus fumigatus / Q000472:pathogenicity*; D001835:Body Weight; D004797:Enzyme-Linked Immunosorbent Assay; D005779:Immunodiffusion / Q000662:veterinary; D008172:Lung Diseases, Fungal / Q000473:pathology / Q000662:veterinary; D011201:Poultry Diseases / Q000382:microbiology* / Q000401:mortality; D014422:Turkeys / Q000382:microbiology*; D014774:Virulence
chemical_list: D000908:Antibodies, Fungal