Effect of a restricted diet on the in vitro glucose-induced insulin release of aging rats.
To study the effect of a sudden loss of body weight on the beta-cell function of aging rats, basal and glucose-induced insulin secretion was measured in pancreatic islets obtained from young (2-month-old), adult (12-month-old) and aging (24-month-old) rats, either fed ad libitum or fed a restricted diet (50% caloric restriction). Basal insulin secretion was similar in islets of young, adult and older rats. Glucose stimulated insulin release was significantly reduced in aging rats as compared to young animals. Animals fed a restricted diet showed a prolonged and higher secretory rate during first phase release when compared to animals fed ad libitum.
Castro
M
M
Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Granada, Spain.
Pedrosa
D
D
Osuna
J I
JI
D000375:Aging / Q000502:physiology*; D000704:Analysis of Variance; D000818:Animals; D004038:Diet, Reducing; D005947:Glucose / Q000494:pharmacology*; D066298:In Vitro Techniques; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D015431:Weight Loss
D007328:Insulin; D005947:Glucose