title: Muscle protein breakdown during endotoxemia in rats and after treatment with interleukin-1 receptor antagonist (IL-1ra).
abstract: The purpose of this study was to examine the effect of endotoxemia on muscle protein degradation and to test the hypothesis that muscle proteolysis during endotoxemia is regulated by interleukin-1 (IL-1). Both total and myofibrillar protein breakdown rates in incubated extensor digitorum longus muscles were increased after the subcutaneous injection of 0.1 or 1.0 mg/kg endotoxin in rats. The endotoxin-induced increase in muscle protein breakdown was blunted by IL-1 receptor antagonist, administered intraperitoneally at a total dose of 45 or 105 mg/kg. Results suggest that endotoxemia in rats gives rise to sepsislike changes in muscle protein breakdown. Increased muscle protein breakdown during endotoxemia may be regulated, at least in part, by IL-1.
authors.affiliation: Department of Surgery, University of Cincinnati, Ohio.
authors.forename: O
authors.initials: O
authors.lastname: Zamir
authors.forename: P O
authors.initials: PO
authors.lastname: Hasselgren
authors.forename: W
authors.initials: W
authors.lastname: O'Brien
authors.forename: R C
authors.initials: RC
authors.lastname: Thompson
authors.forename: J E
authors.initials: JE
authors.lastname: Fischer
mesh_terms: D000818:Animals; D004731:Endotoxins; D053590:Interleukin 1 Receptor Antagonist Protein; D007375:Interleukin-1 / Q000502:physiology*; D009124:Muscle Proteins / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011994:Recombinant Proteins; D012772:Shock, Septic / Q000139:chemically induced / Q000378:metabolism* / Q000628:therapy*; D012795:Sialoglycoproteins / Q000494:pharmacology*
chemical_list: D004731:Endotoxins; D053590:Interleukin 1 Receptor Antagonist Protein; D007375:Interleukin-1; D009124:Muscle Proteins; D011994:Recombinant Proteins; D012795:Sialoglycoproteins