title: Chromosomal abnormalities in patients with Hodgkin's disease: evidence for frequent involvement of the 14q chromosomal region but infrequent bcl-2 gene rearrangement in Reed-Sternberg cells.
abstract: BACKGROUND
  Rearrangements of the bcl-2 gene (also known as BCL2) have been detected in up to 40% of cases of Hodgkin's disease, and it has been speculated that such rearrangements may have a role in the pathogenesis of Hodgkin's disease.
  
  
  OBJECTIVE
  The purposes of this study were (a) to assess the frequency of clonal chromosomal abnormalities in Hodgkin's disease, (b) to identify recurrent changes, (c) to determine whether the bcl-2 gene rearrangement was present in Reed-Sternberg cells (the neoplastic cells of Hodgkin's disease) and their variants, and (d) to analyze whether the presence of t(14;18) translocations in Reed-Sternberg cells explains the observed bcl-2 gene rearrangements in Hodgkin's disease.
  
  
  METHODS
  A cytogenetic study was performed on biopsy specimens from 28 consecutive untreated patients with Hodgkin's disease. The same patients were analyzed for bcl-2 gene rearrangement by a polymerase chain reaction (PCR) technique. To ascertain whether the abnormal karyotypes were present in and restricted to Reed-Sternberg cells, we also performed in situ hybridization with chromosome-specific probes.
  
  
  RESULTS
  Abnormal metaphases were identified in 23 of the 28 patients. In 11 patients, the chromosome 14q region was abnormal; in six of these patients, there was involvement of the 14q32 region that comprises the gene encoding for heavy-chain immunoglobulin. Only one patient had a t(14;18) translocation, whereas almost 40% of these 28 patients showed bcl-2 gene rearrangements by a PCR method. The in situ hybridization method showed that the abnormal karyotype was present in and restricted to Reed-Sternberg cells.
  
  
  CONCLUSIONS
  We conclude that the majority of cases of Hodgkin's disease contain a clonal population with an abnormal karyotype, comprising the Reed-Sternberg cells. The q32 region of chromosome 14 is frequently involved, but a t(14;18) translocation is extremely infrequent. The occurrence of a bcl-2 gene rearrangement in Hodgkin's disease most likely results from the presence of sporadic, small bystander B lymphocytes that carry the translocation and that also can be frequently detected in reactive lymphoid tissue such as tonsils. Also, a range of different chromosomal translocations may provide growth or survival advantages to Reed-Sternberg cells.
authors.affiliation: Department of Pathology, University of Alberta, Edmonton, Canada.
authors.forename: S
authors.initials: S
authors.lastname: Poppema
authors.forename: J
authors.initials: J
authors.lastname: Kaleta
authors.forename: B
authors.initials: B
authors.lastname: Hepperle
mesh_terms: D002883:Chromosomes, Human, Pair 14 / Q000502:physiology*; D015321:Gene Rearrangement; D006689:Hodgkin Disease / Q000235:genetics*; D006801:Humans; D017403:In Situ Hybridization; D007621:Karyotyping; D008677:Metaphase; D016133:Polymerase Chain Reaction; D016539:Reed-Sternberg Cells / Q000166:cytology / Q000648:ultrastructure*; D014178:Translocation, Genetic / Q000235:genetics*