Ascorbic acid biosensor using ascorbate oxidase immobilized on alkylamine glass beads.
A biosensor for ascorbic acid based on enzyme kinetics of ascorbate oxidase (E.C.1.10.3.3) was developed. The enzyme was extracted from Cucurbita maxima, or jerimun and immobilized by covalent bounding, using glutaradehyde as a bifunctional agent, on alkylamine glass beads, with and without enzyme active site protection. A low-cost, home-made oxygen electrode was applied as a transducer. The system has sensitivity from 62.5 up to 500 microM of ascorbic acid with satisfactory operation for more than 2 mo.
Marques
E T
ET
Departamento de Bioquimica, Universidade Federal de Pernambuco, Brazil.
Lima Filho
J L
JL
D000478:Alkylation; D000588:Amines / Q000737:chemistry; D001204:Ascorbate Oxidase / Q000737:chemistry*; D001205:Ascorbic Acid / Q000737:chemistry*; D015374:Biosensing Techniques; D004800:Enzymes, Immobilized; D008863:Microspheres
D000588:Amines; D004800:Enzymes, Immobilized; D001204:Ascorbate Oxidase; D001205:Ascorbic Acid