title: Determination of Penicillium roqueforti toxin by reversed-phase high-performance liquid chromatography.
abstract: A method for the detection and quantification of Penicillium roqueforti toxin (PRT) using reversed-phase high-performance liquid chromatography has been established. The limit of quantitation of this method was 3 ng of PRT, while the limit of detection was 2 ng of toxin. The precision of the analysis based on numerous runs was good. Retention times for PRT were highly reproducible with an average coefficient of variation of about 1.6%. Analysis of PRT in liquid and solid samples showed no interference of the sample matrix. The accuracy of the method was 98.6%, with mean PRT recoveries of 96.8%, and 100.4% for the spiked culture medium and blue cheese extracts, respectively.
authors.affiliation: Manitoba Research Council, University of Manitoba, Food Science Department, Winnipeg, Canada.
authors.forename: K
authors.initials: K
authors.lastname: Siemens
authors.forename: J
authors.initials: J
authors.lastname: Zawistowski
mesh_terms: D002611:Cheese / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000706:statistics & numerical data; D009284:Naphthols / Q000032:analysis*
chemical_list: D009284:Naphthols; C015483:PR Toxin