Membrane fusion of mumps virus with ghost erythrocytes and CV-1 cells.
The octadecyl rhodamine (R18) fluorescent dequenching assay was used to examine membrane fusion between mumps virus and mammalian cells. Rapid fluorescent dequenching, indicative of membrane fusion, was observed when labeled mumps virus was mixed with either ghost erythrocytes or CV-1 cells. After 15 min a saturation limit of 18 virus per erythrocyte ghost and 6400 virus per CV-1 cell was observed. Fetuin was found to inhibit virus fusion, suggesting a role for sialic acid in virus binding to the cells. Two dequenching processes were observed of which the faster process is thought to be membrane fusion and the second process is thought to be probe proximal transfer.
Di Simone
C
C
Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena 91125.
Baldeschwieler
J D
JD
D002460:Cell Line; D004910:Erythrocyte Membrane / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008561:Membrane Fusion; D008856:Microscopy, Fluorescence; D009109:Mumps virus / Q000302:isolation & purification / Q000378:metabolism* / Q000648:ultrastructure; D013696:Temperature