Enhanced stability of YEp plasmids in lager brewing yeasts is related to lager brewing yeast 2-microns DNA.
YEp plasmid stability in the presence of either Saccharomyces cerevisiae laboratory strain 2-microns DNA, or lager brewing yeast 2-microns DNA in the same genetic background, was compared under non-selective culture conditions. It was found that YEp plasmids were more stably maintained in the presence of lager 2-microns DNA under these conditions. By construction of laboratory-lager 2-microns DNA hybrid plasmids, an 867 bp StuI fragment of lager 2-microns DNA was shown to be responsible for the enhanced stability of the YEp plasmid. Nucleotide substitutions at two sites were found by sequencing this region. It was also confirmed that increasing cell ploidy enhanced YEp stability under non-selective conditions.
Hosford
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EA
Central Laboratories for Key Technology, Kirin Brewery Co. Ltd., Kanagawa, Japan.
Sone
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Tanaka
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J
D004271:DNA, Fungal / Q000378:metabolism*; D010957:Plasmids; D011003:Ploidies; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D013045:Species Specificity
D004271:DNA, Fungal