Characterisation of the expression and post-translational processing of the preprotachykinin-I gene and the regulated release of tachykinins by the RINm5F cell-line.
Normal transcription and postranslational processing of the preprotachykinin (PPT)-I gene and regulated release of substance P and neurokinin A by the rat pancreatic endocrine cell-line, RINm5F, has been demonstrated, using radioimmunoassays (RIAs), reversed-phase (rp)HPLC and Northern blot analysis. This is the first stable cell-line found to express the PPT-I gene and provides an opportunity for investigating PPT-I gene expression and tachykinin biosynthesis. RIN5mF cells are a model for the pancreatic beta-cell, which is not known to exhibit PPT-I gene expression which may, therefore, be a feature of the transformed state of these cells. These data may imply that the tachykinins are important in pancreatic islet embryogenesis.
McGregor
G P
GP
Department of Physiology, University of Marburg, Germany.
Hartel
R
R
Fehmann
H C
HC
Lankat-Buttgereit
B
B
Göke
B
B
Göke
R
R
Voigt
K
K
D000818:Animals; D015152:Blotting, Northern; D002851:Chromatography, High Pressure Liquid; D007340:Insulinoma; D011498:Protein Precursors / Q000235:genetics* / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D051381:Rats; D015320:Tachykinins / Q000235:genetics* / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D011498:Protein Precursors; D015320:Tachykinins; C044206:preprotachykinin