[The effect of gamma irradiation on the direct intercellular interaction (rosette formation) of thymic macrophages with thymocytes].
In experiments with (CBA x C57BL/6)F1 mice, the effect of radiation on rosette formation between thymus macrophages (Th-MPh) and thymocytes (Thc) was studied on days 1, 4, 12, 30, and 60 following gamma irradiation with doses of 0.5, 2, 4, and 8 Gy. The influence of supernatants of thymus epithelial cells (EC) on the rosette formation was estimated. Gamma irradiation with doses of above 2 Gy was shown to cause a dose-dependent inhibition of rosette formation of Th-MPh with Thc in vitro. Recovery of rosette-forming ability of Th-MPh was observed on day 60 of the experiment. Two types of rosette-forming Th-MPh were identified: RFMPhII with low rate of binding to Thc and RFMPhII with high rate of binding to Thc. Radiation affects mainly the RFMPhII content. With radiation doses of 4 and 8 Gy no complete restoration of RFMPhI was observed on day 60. The total population of rosette-forming Th-MPh was restored on day 60 mainly due to cells with low rate of rosette formation. The EC supernatant promoted rosette formation of exposed Th-MPh with Thc. The effect was maximum at early times following irradiation of Th-MPh with a dose of 4 Gy.
Beliakov
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Iarilin
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D000818:Animals; D000831:Animals, Newborn; D002448:Cell Adhesion / Q000528:radiation effects; D002450:Cell Communication / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D004847:Epithelial Cells; D004848:Epithelium / Q000528:radiation effects; D005720:Gamma Rays; D008264:Macrophages / Q000166:cytology / Q000528:radiation effects*; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D012397:Rosette Formation; D013950:Thymus Gland / Q000166:cytology / Q000528:radiation effects*; D013997:Time Factors
Vliianie gamma-oblucheniia na priamoe mezhkletochnoe vzaimodeĭstvie (rozetkoobrazovanie) timicheskikh makrofagov s timotsitami.