Diuretic screening in human urine by gas chromatography-mass spectrometry: use of a macroreticular acrylic copolymer for the efficient removal of the coextracted phase-transfer reagent after derivatization by direct extractive alkylation.
A simple and efficient procedure has been developed for the derivatization of diuretic agents in human urine by direct extractive alkylation and their detection by gas chromatography-mass spectrometry. The procedure is an improvement over previous extractive alkylation methods because of the development of a simple clean-up step using a macroreticular acrylic copolymer (SM-7 resin) to remove the coextracted phase-transfer reagent from the organic phase after derivatization. With 1 ml of sample the method gives detection limits in the range 10-50 ng/ml for acetazolamide, probenecid, dichlorphenamide, hydroflumethiazide, furosemide, chlorthalidone, bumetanide, hydrochlorothiazide, quinethazone, bendroflumethiazide, metolazone and cyclopenthiazide.
Lisi
A M
AM
Australian Government Analytical Laboratory, Pymble, N.S.W.
Kazlauskas
R
R
Trout
G J
GJ
D000180:Acrylic Resins; D000478:Alkylation; D004232:Diuretics / Q000652:urine*; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007202:Indicators and Reagents; D011108:Polymers; D000644:Quaternary Ammonium Compounds / Q000737:chemistry
D000180:Acrylic Resins; D004232:Diuretics; D007202:Indicators and Reagents; D011108:Polymers; D000644:Quaternary Ammonium Compounds; C055292:tetrahexylammonium; C027435:tetrapentylammonium; C009405:tetrabutylammonium