Organization of the human monoamine oxidase genes and long-range physical mapping around them.
A 265-kb yeast artificial chromosome containing sequences for human monoamine oxidase A and B (MAO-A and MAO-B) genes has been characterized. These two genes are localized within a region of about 240 kb and are arranged in a tail-to-tail configuration, with the 3' coding sequences separated by about 50 kb. A region about 2.5 Mb around the MAO loci was mapped by pulsed-field gel electrophoresis (PFGE). Comparisons between the restriction maps derived from the YAC and the long-range map derived from genomic digestions were in general agreement. The important features identified include a CpG island at the 5' end of the MAO-A and MAO-B genes, respectively. The combined information supports the order of markers within this region to be DXS77-DXS7-MAOA-MAOB.
Chen
Z Y
ZY
Genetics Laboratory, Biochemistry Department, Oxford, United Kingdom.
Powell
J F
JF
Hsu
Y P
YP
Breakefield
X O
XO
Craig
I W
IW
D015342:DNA Probes; D016521:Electrophoresis, Gel, Pulsed-Field; D015723:Gene Library; D005800:Genes, Fungal; D015894:Genome, Human; D006801:Humans; D008995:Monoamine Oxidase / Q000235:genetics*; D015183:Restriction Mapping; D014960:X Chromosome
D015342:DNA Probes; D008995:Monoamine Oxidase