Identification of an immediate early gene, pghs-B, whose protein product has prostaglandin synthase/cyclooxygenase activity.
We have characterized a growth factor-inducible gene, pghs-B, isolated by differential screening of a lambda complementary DNA library from RNA of serum-stimulated NIH 3T3 cells which encodes a 604-amino acid protein presenting high similarity with prostaglandin G/H synthase (PGHS). Induction of both pghs-B mRNA and protein is rapid and remains at high levels for several hours. The increase in pghs-B mRNA is mainly due to transcriptional activation of the gene. PGHS-B has been expressed in the baculovirus system and has been demonstrated to be involved in prostaglandin synthesis and to be inhibited by indomethacin, demonstrating that PGHS-B is indeed an enzyme related to PGHS. The pghs-B gene maps to the [1G-1H2] region of the murine genome.
Ryseck
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RP
Department of Molecular Biology, Bristol-Myers Squibb Pharmaceutical Research Institute, Princeton, New Jersey 08543-4000.
Raynoschek
C
C
Macdonald-Bravo
H
H
Dorfman
K
K
Mattéi
M G
MG
Bravo
R
R
D016475:3T3 Cells / Q000187:drug effects / Q000201:enzymology; D000595:Amino Acid Sequence; D000818:Animals; D016367:Baculoviridae / Q000235:genetics; D001483:Base Sequence; D002874:Chromosome Mapping; D051546:Cyclooxygenase 2; D004247:DNA / Q000235:genetics; D004790:Enzyme Induction; D005796:Genes; D005822:Genetic Vectors; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C / Q000235:genetics; D008817:Mice, Mutant Strains / Q000235:genetics; D008969:Molecular Sequence Data; D011451:Prostaglandin-Endoperoxide Synthases / Q000096:biosynthesis / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D014158:Transcription, Genetic
D011993:Recombinant Fusion Proteins; D004247:DNA; C077623:Ptgs2 protein, mouse; D051546:Cyclooxygenase 2; D011451:Prostaglandin-Endoperoxide Synthases