Continuous scan cyclic voltammetry (CSCV): a new high-speed electrochemical method for monitoring neuronal dopamine release.
This report describes a new form of fast cyclic voltammetry which samples electrochemical reactions of dopamine at one every 10 ms. We have called this technique 'continuous scan cyclic voltammetry' (CSCV). The technique uses a carbon fibre microelectrode which is connected to a continuously active sine-wave source. The applied voltage is a 100 Hz precision sine wave. Faradaic signals due to cycles of continuous oxidation and reduction of dopamine at the tip of the electrode can be observed superimposed on a fixed charging current. The technique is insensitive to moderate concentrations of ascorbate as a depletion zone for ascorbate is rapidly established around the electrode. In a brain slice preparation the technique can show the time course of dopamine release on a millisecond time scale. It should prove a valuable new tool for the investigation of dopamine transmission in the brain.
Millar
J
J
Department of Pharmacology, Queen Mary and Westfield College, London, UK.
O'Connor
J J
JJ
Trout
S J
SJ
Kruk
Z L
ZL
D000818:Animals; D001205:Ascorbic Acid / Q000378:metabolism; D015306:Biogenic Monoamines / Q000378:metabolism; D001923:Brain Chemistry / Q000502:physiology; D002384:Catalysis; D004298:Dopamine / Q000378:metabolism*; D004563:Electrochemistry; D004594:Electrophysiology; D007700:Kinetics; D009474:Neurons / Q000378:metabolism*; D009714:Nucleus Accumbens / Q000502:physiology; D010084:Oxidation-Reduction; D051381:Rats
D015306:Biogenic Monoamines; D001205:Ascorbic Acid; D004298:Dopamine