[Experimental urinary tract infection caused by Escherichia coli in diabetic mouse--uroepithelial colonization and polymorphonuclear leukocyte function].
Relationship between polymorphonuclear leukocyte (PMN) function and susceptibility to infections in diabetic mice was studied. In the experimental urinary tract infections, 6 strains of Escherichia coli with different fimbrial expression and hemolysin production were used. In contrast with non-fimbriated strains, fimbriated strains were more susceptible to phagocytosis by PMN. MS+.MR+ strains, however, showed resistance to intracellular killing by PMN. OEC-709, OEC-716 (MS+.MR+ strains), and OEC-734 (MS+.MR- strains), also showed resistance to the bactericidal activity of the serum. The killing activities of PMN derived from diabetic mice against these 3 strains were significantly lower than those of PMN from normal mice. In normal mice, MS+.MR+ strains showed the strongest colonization ability to the bladder and kidney, whereas MS-.MR- strains showed the weakest ability among the strains. In diabetic mice, the number of bacteria colonizing to the bladder and kidney was higher than that in normal mice, except for one MS-.MR- strain. Significant differences were observed in the strains (OEC-709, 716, 734), in which PMN was concerned strongly in the bacterial killing. Finally, these findings suggest that deterioration of the bactericidal activity of PMN in a diabetic state is involved in increased susceptibility of diabetic mice.
Kishi
M
M
Department of Urology, Okayama University Medical School.
D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000150:complications / Q000276:immunology*; D004198:Disease Susceptibility; D004848:Epithelium / Q000382:microbiology; D004927:Escherichia coli Infections / Q000150:complications / Q000276:immunology / Q000382:microbiology*; D005260:Female; D051379:Mice; D008813:Mice, Inbred ICR; D009504:Neutrophils / Q000502:physiology*; D010587:Phagocytosis; D014551:Urinary Tract / Q000382:microbiology*; D014552:Urinary Tract Infections / Q000150:complications / Q000276:immunology / Q000382:microbiology*