Simple method for isolation of primordial germ cells from chick embryos.
A simple one step centrifugation method was developed for purification of primordial germ cells (PGCs) of chick embryos. PGCs, constituting less than 0.1% of the total blood cells of stage 13-14 embryos that contained a microliter amount of blood, were concentrated at the interface of a 6.3% (w/v) and 14.4% (w/v) Ficoll bilayer by centrifugation at 800 x g for 30 min. the purity of these PGCs was 86%, which was 22 times that obtained previously.
Chang
I K
IK
RIKEN Cell Bank, Institute of Physical and Chemical Research, Ibaraki, Japan.
Tajima
A
A
Yasuda
Y
Y
Chikamune
T
T
Ohno
T
T
D000818:Animals; D002469:Cell Separation / Q000379:methods*; D002498:Centrifugation; D002642:Chick Embryo; D005362:Ficoll; D005854:Germ Cells; D012965:Sodium Chloride
D005362:Ficoll; D012965:Sodium Chloride