Chromosome painting using chromosome-specific probes from flow-sorted pig chromosomes.
Biotinylated chromosome-specific probes were prepared from flow-sorted pig chromosomes 1, 13, 18, X, and Y using the degenerate oligonucleotide-primed polymerase chain reaction. Probes prepared in this way can be used to confirm the identity of chromosomes in the bivariate pig flow karyotype and in pig x mouse somatic cell hybrids.
Langford
C F
CF
AFRC Institute of Animal Physiology and Genetics Research, Babraham, Cambridge, UK.
Telenius
H
H
Carter
N P
NP
Miller
N G
NG
Tucker
E M
EM
D000818:Animals; D001483:Base Sequence; D005434:Flow Cytometry; D006822:Hybrid Cells; D017403:In Situ Hybridization; D007621:Karyotyping / Q000379:methods*; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes / Q000235:genetics*; D016133:Polymerase Chain Reaction; D013552:Swine
D015345:Oligonucleotide Probes