[Biochemical study on steroid sulfatase and its clinical application to the obstetrics and gynecology].
Steroid sulfatase desulfates a number of 3 beta-hydroxysteroid sulfates, converting inactive steroid hormone to the active form. I have developed an enzyme-linked immunosorbent assay (ELISA) using polyclonal antibody against the sulfatase which was purified from human placenta to measure an amount of the enzyme protein in sera of gynecologic cancer patients. By this method, it was found that the serum steroid sulfatase level is significantly elevated in patients with endometrial carcinoma (p less than 0.05) and ovarian carcinoma (p less than 0.01) as compared to that of normal women. Steroid sulfatase deficiency, X-linked ichthyosis (XLI) is an inherited skin disorder. The sulfatase gene and the enzyme protein were examined in patients with XLI. When the first and last (exon 10) exons of the sulfatase gene were amplified by PCR using patients' genomic DNA as templates, no product was detected in all six cases examined. In addition, neither mRNA of the sulfatase nor the enzyme protein was detected in a patient with XLI. These observations suggest that most Japanese XLI patients are caused by an extensive deletion of the steroid sulfatase gene.
Sugawara
T
T
Department of Obstetrics and Gynecology, Hokkaido University School of Medicine, Sapporo, Japan.
D001192:Arylsulfatases / Q000097:blood* / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D017353:Gene Deletion; D015971:Gene Expression Regulation, Enzymologic; D005833:Genital Neoplasms, Female / Q000175:diagnosis*; D006801:Humans; D016114:Ichthyosis, X-Linked / Q000201:enzymology / Q000235:genetics; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis; D043266:Steryl-Sulfatase
D012333:RNA, Messenger; D001192:Arylsulfatases; D043266:Steryl-Sulfatase