Interleukin 6 modulation of second messenger systems in anterior pituitary cells.
We investigated the effect of interleukin-6 (IL-6) on second messenger systems in anterior pituitary (AP) cells. The acute exposition of membranes derived from the pituitary gland to IL-6 did not modify basal and forskolin-stimulated adenylate cyclase (AC) activity, as well as inositol phosphate (IP) production and free [Ca(++)]i. Preincubation of AP cells with IL-6 for 20 min did not affect basal second messengers levels, while completely abolished the stimulation by VIP of AC activity, partially inhibited forskolin-stimulated cAMP formation and reduced TRH-stimulated IP production. Finally, the pretreatment of AP cells for 20 min with IL-6 also reduced the TRH-induced rise in free [Ca(++)]i.
Grimaldi
M
M
Dip. di Scienza delle Comunicazioni Umane, II Facoltà di Medicina e Chirurgia, Università di Napoli, Italy.
Meucci
O
O
Scorziello
A
A
Florio
T
T
Ventra
C
C
De Mercato
R
R
Schettini
G
G
D000262:Adenylyl Cyclases / Q000378:metabolism; D000704:Analysis of Variance; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D005260:Female; D007295:Inositol Phosphates / Q000378:metabolism; D015850:Interleukin-6 / Q000502:physiology*; D010903:Pituitary Gland, Anterior / Q000166:cytology / Q000201:enzymology / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D015290:Second Messenger Systems / Q000502:physiology*; D013997:Time Factors
D007295:Inositol Phosphates; D015850:Interleukin-6; D000262:Adenylyl Cyclases; D002118:Calcium