Evaluation of three rapid enzyme immunoassays and cell culture for detection of respiratory syncytial virus.
Three rapid enzyme immunoassay techniques for the detection of respiratory syncytial virus antigen (Becton Dickinson Directigen RSV, Abbott RSV Testpack and Abbott RSV EIA) and cell culture were evaluated in a total of 250 nasal washings. The sensitivity and specificity were 62% and 76% respectively for Directigen, 64% and 86% for RSV Testpack, and 76% and 81% for RSV EIA, taking cell culture as the reference method. Agreement between cell culture and EIA techniques was 79% (70 positive and 128 negative results). All three EIA techniques gave positive results in 69 samples (52 positive and 17 negative in the cell culture). In 121 samples all three EIA techniques gave negative results (103 negative and 18 positive in the cell culture). Using the cell culture technique 46 strains other than respiratory syncytial virus were isolated.
Mendoza
J
J
Servicio de Microbiologia, Hospital Regional de Especialidades Virgen de las Nieves, Granada, Spain.
Rojas
A
A
Navarro
J M
JM
Plata
C
C
de la Rosa
M
M
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis*; D005069:Evaluation Studies as Topic; D006801:Humans; D007124:Immunoenzyme Techniques; D007223:Infant; D007231:Infant, Newborn; D009666:Nose / Q000382:microbiology*; D011446:Prospective Studies; D012136:Respiratory Syncytial Viruses / Q000276:immunology / Q000302:isolation & purification*; D010253:Respirovirus Infections / Q000382:microbiology*; D012680:Sensitivity and Specificity; D014709:Vero Cells
D000956:Antigens, Viral