Postnatal development of hepatic xanthine oxidase in baby pigs and turnover of purine catabolites at reduced ambient temperature.
1. The activity of xanthine oxidase in liver samples of baby pigs up to 4 weeks of age was investigated. On the 3rd day of life the turnover of hypoxanthine and of uric acid were measured after intravenous injection of 3H- and 14C-labelled tracers into animals kept at normal (32 degrees C) and reduced (20 degrees C) ambient temperature. 2. Hepatic xanthine oxidase activity increased progressively from 2 to 28 days of age (r = 0.689; P < 0.001). The increase of Vmax and of KM within 3-4 weeks was about 4.5-fold. 3. In 3-day-old baby pigs kept at normal temperature, pool size and turnover was about 10-fold higher for hypoxanthine than for uric acid. 4. At reduced ambient temperature, the pool size of uric acid increased 3.9-fold (P < 0.01) and turnover 1.6-fold (P < 0.05). For hypoxanthine the increases were insignificant.
Giesecke
D
D
Institut für Physiologie, Physiologische Chemie und Ernährungsphysiologie, Universität München, Germany.
Heiss
G
G
Erhardt
W
W
Rosskopf
R
R
D000818:Animals; D000831:Animals, Newborn; D003080:Cold Temperature / Q000009:adverse effects; D019271:Hypoxanthine; D007042:Hypoxanthines / Q000378:metabolism; D007674:Kidney Diseases / Q000209:etiology / Q000378:metabolism / Q000662:veterinary; D007700:Kinetics; D008099:Liver / Q000254:growth & development / Q000378:metabolism*; D011687:Purines / Q000378:metabolism*; D013552:Swine; D013553:Swine Diseases / Q000209:etiology / Q000378:metabolism; D014527:Uric Acid / Q000378:metabolism; D014969:Xanthine Oxidase / Q000378:metabolism*
D007042:Hypoxanthines; D011687:Purines; D014527:Uric Acid; D019271:Hypoxanthine; D014969:Xanthine Oxidase