Diacylglycerol formation from phosphatidylcholine in angiotensin II-stimulated vascular smooth muscle cells.
In cultured vascular smooth muscle cells (VSMC), angiotensin II (Ang II) induces a biphasic diacylglycerol (DAG) formation peaking at 15 sec and 5 min. Although it has been well established that the first peak is produced by the hydrolysis of inositol 4,5-bisphosphate (PIP2), the origin of the second DAG peak has never been examined in detail. In the present paper, we provide evidence that the second peak of DAG formation in Ang II-stimulated VSMC originates mainly from PC.
Kondo
T
T
Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN 37232.
Konishi
F
F
Inui
H
H
Inagami
T
T
D000804:Angiotensin II / Q000494:pharmacology*; D000818:Animals; D002478:Cells, Cultured; D004075:Diglycerides / Q000378:metabolism*; D066298:In Vitro Techniques; D009131:Muscle, Smooth, Vascular / Q000378:metabolism*; D010713:Phosphatidylcholines / Q000378:metabolism*; D011493:Protein Kinase C / Q000502:physiology*; D051381:Rats; D011921:Rats, Inbred WKY
D004075:Diglycerides; D010713:Phosphatidylcholines; D000804:Angiotensin II; D011493:Protein Kinase C