Treatment with excitatory amino acids or high K+ and NMDA receptors in cerebellar granule cells.
N-methyl-D-aspartate (NMDA) receptor activity was examined, in terms of 45Ca2+ influx, during the development of cerebellar granule cells grown under 'non-trophic' [10 mM potassium (K10)] or 'trophic' conditions [25 mM potassium (K25), NMDA and kainate (KA)]. NMDA receptor activity increased sharply between 2 and 4 days in vitro (DIV) irrespective of growth conditions which upon further cultivation exerted a powerful influence, the NMDA response increasing progressively in K25 and NMDA grown cells, while remaining at a constant level in KA treated cells. In contrast, in K10 grown cells the NMDA response declined by 7 DIV to about 20% of the estimates in K25 at 9 DIV. Trophic conditions are, therefore, essential for the proper functional expression of NMDA receptors.
Resink
A
A
Netherlands Institute for Brain Research, Amsterdam.
Boer
G J
GJ
Balázs
R
R
D000596:Amino Acids / Q000494:pharmacology*; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002132:Calcium Radioisotopes; D002478:Cells, Cultured; D002531:Cerebellum / Q000737:chemistry / Q000166:cytology / Q000187:drug effects*; D007608:Kainic Acid / Q000494:pharmacology; D016202:N-Methylaspartate / Q000494:pharmacology; D009474:Neurons / Q000737:chemistry / Q000187:drug effects*; D011188:Potassium / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D016194:Receptors, N-Methyl-D-Aspartate / Q000187:drug effects* / Q000378:metabolism
D000596:Amino Acids; D002132:Calcium Radioisotopes; D016194:Receptors, N-Methyl-D-Aspartate; D016202:N-Methylaspartate; D011188:Potassium; D007608:Kainic Acid; D002118:Calcium