A model for fetal cleft lip repair in lambs.
Fetal wounds heal without inflammation and scar formation. This phenomenon may, in the future, be applicable to human cleft lip and palate repair. However, extensive experimental work must first be done to document the benefits of in utero repair. We developed a large animal model for creation and repair of a complete cleft lip and alveolus using fetal lambs. The cleft lip and alveolus deformity was created in eight 75-day-gestation fetuses (term = 145 days) and either repaired in three layers or left unrepaired. There were four sham-operated fetuses, and all animals were alive at harvest. Repaired, unrepaired, and control fetuses were harvested at 7, 14, 21, and 70 days following surgery. The unrepaired fetuses demonstrated a complete cleft lip and alveolus with an oronasal fistula. The maxilla was asymmetrical, with the greater segment deviated toward the cleft and with decreased anterior maxillary width. In contrast, repaired cleft lip and alveolus animals showed no scar, normal thickness of the lip, and a symmetrical maxilla. Histologic analysis of the repaired wounds showed evidence of tissue regeneration without scar formation. The results of this preliminary study indicate that the fetal lamb cleft lip and alveolus model is technically feasible with an excellent survival rate. Healing occurs without scar formation. In the repaired animals, the maxilla was symmetrical. This model will be used to document facial growth following in utero repair of a cleft lip and alveolus.
Longaker
M T
MT
Fetal Treatment Program, University of California, San Francisco.
Stern
M
M
Lorenz
P
P
Whitby
D J
DJ
Dodson
T B
TB
Harrison
M R
MR
Adzick
N S
NS
Kaban
L B
LB
D000539:Alveolar Process / Q000002:abnormalities; D000818:Animals; D002971:Cleft Lip / Q000209:etiology / Q000601:surgery*; D004195:Disease Models, Animal; D005260:Female; D005333:Fetus / Q000601:surgery*; D011247:Pregnancy; D012756:Sheep; D013518:Surgery, Plastic / Q000379:methods*; D013536:Suture Techniques; D014599:Uterus; D014945:Wound Healing / Q000502:physiology*