title: Quantitative analysis of mercaptoundecahydrododecaborate by Fourier transform infrared spectroscopy.
abstract: Mercaptoundecahydrododecaborate (BSH) is an important agent in boron neutron capture therapy (BNCT) of various cancers. A simple and rapid analytical method for the measurement of mercaptoundecahydrododecaborate in aqueous solution and in urine by Fourier transform infrared spectroscopy has been developed. A thin-pathlength sampling apparatus was used to minimize the strong absorption of water. The subtraction of water absorbance from sample spectra resolved a B-H band at 2493 cm-1. The quantitative measurement of BSH concentration was carried out by integrating the B-H band above baseline in the range of 2534-2440 cm-1. The lower limit of measuring the concentration of sodium BSH (Na2B12H11SH) in our experiment was 10 micrograms/ml (about 5 ppm of boron). This method measures the hydroborate (B-H) concentration instead of total boron and, thus, may be utilized to measure the BSH concentration in in vivo samples for metabolic studies.
authors.affiliation: Idaho State University, College of Pharmacy, Pocatello 83209.
authors.forename: D R
authors.initials: DR
authors.lastname: Lu
authors.forename: C
authors.initials: C
authors.lastname: Munro
mesh_terms: D000818:Animals; D001894:Borohydrides / Q000652:urine*; D005583:Fourier Analysis; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013055:Spectrophotometry, Infrared; D013438:Sulfhydryl Compounds / Q000652:urine*
chemical_list: D001894:Borohydrides; D013438:Sulfhydryl Compounds; C014651:mercaptoundecahydrododecaborate