Development and validation of a high-performance liquid chromatographic method for the determination of benzophenone-3 in rats.
A precise, accurate, selective and sensitive liquid chromatographic method for the determination of benzophenone-3 in rat biological fluids and different tissues has been developed. The minimum detection limit for benzophenone-3 was 2.0 ng ml-1 and the retention time was 6.01 min. Standard curves for benzophenone-3 were linear in a wide range of concentrations in methanol and different body fluids, ranging from 6.25 ng ml-1 to 100 micrograms ml-1. To detect benzophenone-3 in rat whole blood after oral administration, HCl hydrolysis was required. Benzophenone-3 was found to produce a peak blood concentration 1 h after administration. Free benzophenone-3 in urine represented a very small percentage during the first 12 h after administration, while a higher concentration of the glucuronide conjugate was detected in the same time period.
Abdel-Nabi
I M
IM
Pharmacology and Toxicology Department, New Jersey Medical School, Newark.
Kadry
A M
AM
Davis
R A
RA
Abdel-Rahman
M S
MS
D000818:Animals; D001577:Benzophenones / Q000032:analysis* / Q000493:pharmacokinetics; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D008297:Male; D008970:Molecular Weight; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014018:Tissue Distribution / Q000502:physiology
D001577:Benzophenones; C047723:benzophenone