Fusion of phospholipid vesicles induced by phospholipase D in the presence of calcium ion.
Fusion of phospholipid vesicles composed of various combination of phosphatidylcholine, phosphatidylethanolamine and phosphatidic acid was induced by Ca2+ in the presence or absence of phospholipase D from Streptomyces chromofuscus. For the vesicles composed of 20:50:30 molar ratio of phosphatidylcholine/phosphatidylethanolamine/phosphatidic acid, the initial fusion rate was much faster than the expected value when only the conversion of phosphatidylcholine and phosphatidylethanolamine into phosphatidic acid by phospholipase D is taken into account. These observations are discussed in terms of the involvement of outer monolayer of the vesicles and the enzyme activity itself in the fusion process.
Park
J B
JB
Department of Life Science, Korea Advanced Institute of Science and Technology, Yuseong-Gu, Taejon.
Lee
T H
TH
Kim
H
H
D002118:Calcium / Q000494:pharmacology*; D004357:Drug Synergism; D008081:Liposomes; D008561:Membrane Fusion / Q000187:drug effects*; D010712:Phosphatidic Acids / Q000378:metabolism; D010713:Phosphatidylcholines / Q000378:metabolism; D010714:Phosphatidylethanolamines / Q000378:metabolism; D010739:Phospholipase D / Q000494:pharmacology*
D008081:Liposomes; D010712:Phosphatidic Acids; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D010739:Phospholipase D; D002118:Calcium