title: Factors from fluid of the ovarian pocket that stimulate sperm motility in domestic hens.
abstract: Fluid was collected from the region of the peritoneum surrounding the ovarian pocket of domestic hens at about the time of ovulation. This fluid, diluted to 10%, increased the motility of chicken spermatozoa by a maximum of sixfold at 40 degrees C in vitro. Gel filtration revealed two peaks of motility-stimulating activity: one was identified as calcium and the other as a heat-labile substance of low M(r) (200). It is suggested that this motility-stimulating activity may facilitate fertilization, as spermatozoa are normally stored in a quiescent state within the oviduct and can be passively transported by cilia to the site of fertilization in the ovarian pocket.
authors.affiliation: Laboratory of Animal Reproduction, Faculty of Agriculture, Miyazaki University, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Ashizawa
authors.forename: G J
authors.initials: GJ
authors.lastname: Wishart
mesh_terms: D000818:Animals; D001685:Biological Factors / Q000378:metabolism*; D002645:Chickens / Q000502:physiology*; D002850:Chromatography, Gel; D005260:Female; D005306:Fertilization / Q000502:physiology; D008297:Male; D008970:Molecular Weight; D010053:Ovary / Q000378:metabolism*; D013081:Sperm Motility / Q000502:physiology*; D013094:Spermatozoa / Q000502:physiology
chemical_list: D001685:Biological Factors