[Microbiological production of 3-oxo-bisnorchola-1, 4-dien-22-oic acid from cholesterol by an Arthrobacter 82].
Among nineteen strains of Arthrobacter which showed to be able to decompose cholesterol in preliminary experiments, a strain of Arthrobacter 82 was selected for microbiological production of 3-oxo-bisnorchola-1,4-dien-22-oic-acid (BNC) from cholesterol. The yield is over of 50% weight percent concentration of 0.25% in the presence of cobalt sulfate. The main intermediate in such a conversion process is cholestenone. Lower glucose and higher corn steep liquor concentration were favorable for side chain degradation of cholestenone and more BNC could be produced. BNC was crystallized in acidic solution and obtained by centrifugation. The structure and characteristics of BNC has been identified by means of conventional physical, chemical and spectrometric techniques.
Fa
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Institute of Microbiology, Academia Sinica, Beijing.
Su
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D001173:Arthrobacter / Q000378:metabolism*; D001673:Biodegradation, Environmental; D002783:Cholestenones / Q000378:metabolism; D002784:Cholesterol / Q000378:metabolism*; D013256:Steroids / Q000378:metabolism*
C077549:3-oxo-bisnorchola-1,4-dien-22-oic acid; D002783:Cholestenones; D013256:Steroids; D002784:Cholesterol