A rapid and reliable method to create tandem arrays of short DNA sequences.
Tandemly polymerized regulatory elements, antisense RNA segments or ribozymes are potentially useful in selective gene silencing. However, existing methods of tandemly polymerizing short DNA segments are laborious. We present a procedure that can create cloned arrays of 40-70 monomer units in two steps. We have created long arrays of regulatory elements and potential ribozyme sequences. Silencing of human immunodeficiency virus (HIV-1) activation by tandem arrays of a regulatory element in human immune system cells and in other human and monkey cells is discussed.
Graham
G J
GJ
Department of Cell Biology, Albert Einstein College of Medicine, Bronx, NY 10461.
Maio
J J
JJ
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000138:chemical synthesis; D004256:DNA Polymerase I; D004926:Escherichia coli; D008969:Molecular Sequence Data; D010957:Plasmids; D011108:Polymers; D012045:Regulatory Sequences, Nucleic Acid; D012091:Repetitive Sequences, Nucleic Acid; D012270:Ribosomes
D011108:Polymers; D004247:DNA; D004256:DNA Polymerase I