Abnormal auditory brainstem responses and cochlear pathology in rats induced by an exaggerated styrene exposure regimen.
Groups of 12 male 42-day-old rats were exposed to 0 or 800 ppm styrene vapors for 14 hr/day, 5 days/week for 3 weeks. Tone-pip auditory brainstem responses (ABRs) at 4, 8, 16, and 30 kHz were obtained after the last exposure. ABRs were minimally affected at 4 kHz and moderately to severely affected at 8, 16, and 30 kHz as indicated by waveforms which had a decreased amplitude and increased latency as compared to the controls. Missing outer hair cell(s) were evident in the basal and lower middle turns of the organ of Corti. Outer hair cell loss was least in the first row and greatest in the second and third rows. Occasional inner hair cells were also missing in regions of severe outer hair cell loss. The distribution of hair cell loss within the cochlea was consistent with the pattern of ABR alterations. These data document mid-frequency auditory dysfunction in styrene-exposed young adult rats with significant damage to the organ of Corti following an exaggerated styrene exposure regimen.
Yano
B L
BL
Toxicology Research Laboratory, Dow Chemical Company, Midland, Michigan 48674.
Dittenber
D A
DA
Albee
R R
RR
Mattsson
J L
JL
D000818:Animals; D015834:Cochlear Diseases / Q000139:chemically induced* / Q000473:pathology; D016057:Evoked Potentials, Auditory, Brain Stem / Q000502:physiology*; D006198:Hair Cells, Auditory / Q000187:drug effects; D008297:Male; D008855:Microscopy, Electron, Scanning; D009925:Organ of Corti / Q000473:pathology; D051381:Rats; D011916:Rats, Inbred F344; D020058:Styrene; D013343:Styrenes / Q000633:toxicity*
D013343:Styrenes; D020058:Styrene