title: Increase in cardiac muscle fructose content in streptozotocin-induced diabetic rats.
abstract: To evaluate the activation of the sorbitol pathway in cardiac muscle in diabetic rats, we measured sorbitol, fructose, and myo-inositol content in cardiac tissue obtained from control and streptozotocin-diabetic rats, with or without an 8-week insulin treatment, using gas chromatography-mass spectrometry (GC-MS). Cardiac fructose and sorbitol content in 10-week diabetic rats increased by 60-fold and 3.9-fold of those of control rats, respectively (P less than .001). In contrast, cardiac myo-inositol content in 10-week diabetic rats decreased to 56% (P less than .025) of the control value. The abnormalities in cardiac fructose, sorbitol, and myo-inositol content were completely normalized by the 8-week insulin treatment, which was initiated 2 weeks after the induction of diabetes. There was no difference in cardiac aldose reductase activity between control and diabetic rats. However, cardiac sorbitol dehydrogenase activity in diabetic rats was 151% (P less than .005) higher than that of control rats, although hepatic sorbitol dehydrogenase activity was not different between the two groups. These results indicate that the sorbitol pathway is significantly activated in cardiac tissue obtained from streptozotocin-induced diabetic rats, which results in the marked cardiac accumulation of fructose.
authors.affiliation: Third Department of Medicine, Shiga University of Medical Science, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Kashiwagi
authors.forename: T
authors.initials: T
authors.lastname: Obata
authors.forename: M
authors.initials: M
authors.lastname: Suzaki
authors.forename: Y
authors.initials: Y
authors.lastname: Takagi
authors.forename: Y
authors.initials: Y
authors.lastname: Kida
authors.forename: T
authors.initials: T
authors.lastname: Ogawa
authors.forename: Y
authors.initials: Y
authors.lastname: Tanaka
authors.forename: T
authors.initials: T
authors.lastname: Asahina
authors.forename: M
authors.initials: M
authors.lastname: Ikebuchi
authors.forename: Y
authors.initials: Y
authors.lastname: Saeki
mesh_terms: D000449:Aldehyde Reductase / Q000378:metabolism; D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D005632:Fructose / Q000032:analysis* / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry; D007294:Inositol / Q000032:analysis / Q000378:metabolism; D007064:L-Iditol 2-Dehydrogenase / Q000378:metabolism; D008297:Male; D010210:Papillary Muscles / Q000737:chemistry* / Q000201:enzymology / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013012:Sorbitol / Q000032:analysis / Q000378:metabolism; D013311:Streptozocin
chemical_list: D005632:Fructose; D007294:Inositol; D013012:Sorbitol; D013311:Streptozocin; D007064:L-Iditol 2-Dehydrogenase; D000449:Aldehyde Reductase