title: Effects of split-thickness and full-thickness skin grafts on secondary graft contraction in horses.
abstract: Full-thickness, circular (4-cm diameter) cutaneous wounds were created on the metacarpi and metatarsi of 6 horses. Immediately after wounding, 1 wound on each horse received a meshed, split-thickness skin graft (0.64 mm) obtained from the ventrolateral aspect of the horse's thorax by use of a pneumatic dermatome, whereas a second wound received a meshed, full-thickness skin graft obtained from the pectoral area. In addition, sections of split-thickness and full-thickness grafts were refrigerated in a solution of McCoy's 5A medium, to which equine serum (10%) and gentamicin sulfate solution (16 mg/dl) were added. Ten days after wounding, 1 granulating wound on each horse was grafted with a stored, meshed, split-thickness graft, and 1 granulating wound on each horse was grafted with a stored, meshed, full-thickness graft. Areas of wounds were calculated from photographs taken of wounds on days 1, 5, 10, 15, 20, 25, and 30 after wounding. Time course of contraction was determined by use of a first-order mathematic model of changes in area through time. Rate constants of contraction for fresh or granulating wounds receiving full-thickness grafts did not differ significantly from those for fresh or granulating wounds receiving split-thickness grafts. Rate constants of contraction for grafted fresh wounds, however, were significantly less than those of grafted granulating wounds, regardless of whether a split-thickness or full-thickness graft was applied.
authors.affiliation: Department of Large Animal Medicine and Surgery, College of Veterinary Medicine, College Station, TX 77843-4475.
authors.forename: T S
authors.initials: TS
authors.lastname: Ford
authors.forename: J
authors.initials: J
authors.lastname: Schumacher
authors.forename: G W
authors.initials: GW
authors.lastname: Brumbaugh
authors.forename: C M
authors.initials: CM
authors.lastname: Honnas
mesh_terms: D000818:Animals; D005260:Female; D006097:Granulation Tissue / Q000502:physiology; D006736:Horses / Q000502:physiology / Q000601:surgery*; D007700:Kinetics; D011897:Random Allocation; D016038:Skin Transplantation / Q000662:veterinary*; D014182:Transplantation, Autologous; D014945:Wound Healing / Q000502:physiology*