title: Direct evidence for protein kinase C involvement in insulin-stimulated hexose uptake.
abstract: Insulin has been reported to translocate protein kinase C (PKC) in rat adipocytes, and activation of PKC by phorbol esters is known to increase hexose uptake in these cells (1.2). To test the hypothesis that PKC may participate in insulin-stimulated hexose uptake, adipocytes were partially depleted of protein kinase C by overnight phorbol ester treatment, thereby impairing insulin effects on hexose uptake. Purified PKC was then introduced into these PKC-depleted adipocytes by electropermeabilization, and this fully restored insulin-stimulated hexose uptake. These findings provide direct evidence that PKC is required for insulin-stimulated hexose uptake.
authors.affiliation: Research Service, James A. Haley Veterans Administration Hospital, Tampa, FL 33612.
authors.forename: D R
authors.initials: DR
authors.lastname: Cooper
authors.forename: J E
authors.initials: JE
authors.lastname: Watson
authors.forename: H
authors.initials: H
authors.lastname: Hernandez
authors.forename: B
authors.initials: B
authors.lastname: Yu
authors.forename: M L
authors.initials: ML
authors.lastname: Standaert
authors.forename: D K
authors.initials: DK
authors.lastname: Ways
authors.forename: T T
authors.initials: TT
authors.lastname: Arnold
authors.forename: T
authors.initials: T
authors.lastname: Ishizuka
authors.forename: R V
authors.initials: RV
authors.lastname: Farese
mesh_terms: D000273:Adipose Tissue / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002478:Cells, Cultured; D003847:Deoxyglucose / Q000378:metabolism*; D004558:Electric Stimulation; D005990:Glycerol / Q000378:metabolism; D008297:Male; D009004:Monosaccharide Transport Proteins / Q000378:metabolism*; D011493:Protein Kinase C / Q000378:metabolism*; D051381:Rats; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
chemical_list: D009004:Monosaccharide Transport Proteins; D003847:Deoxyglucose; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate; D005990:Glycerol