Post-transcriptional regulation by interferon-alpha of epsilon-globin production in human erythroleukemia K-562 cells.
Treatment of human K-562 erythroleukemia cells with interferon-alpha (IFN-alpha) resulted in a six-fold increase in the production of epsilon-globin, a component of embryonic hemoglobin, but in a two-fold or less augmentation of the production of other globin species. There was no comparable increase in the production of epsilon-globin mRNA following IFN treatment. We did, however, observe a shift in the cytoplasmic location of the epsilon-globin mRNA from the messenger ribonucleoprotein (mRNP) to the polysome fraction after IFN-alpha addition. No such shift was noted in the location of the mRNA for A gamma-globin, the major globin product of K-562 cells, which was always present in the polysome fraction. These results are significant with respect to the mechanisms of cellular regulation and differentiation induced by IFNs.
Friedman
R M
RM
Department of Pathology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814.
Yeh
A
A
Tang
W
W
D000911:Antibodies, Monoclonal; D003600:Cytosol / Q000378:metabolism*; D015342:DNA Probes; D004273:DNA, Neoplasm / Q000032:analysis; D005914:Globins / Q000096:biosynthesis* / Q000235:genetics; D006454:Hemoglobins / Q000032:analysis; D006801:Humans; D016898:Interferon-alpha / Q000494:pharmacology*; D004915:Leukemia, Erythroblastic, Acute / Q000378:metabolism*; D011132:Polyribosomes / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis*; D012334:RNA, Neoplasm / Q000032:analysis; D012261:Ribonucleoproteins / Q000378:metabolism; D014158:Transcription, Genetic / Q000235:genetics*; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D015342:DNA Probes; D004273:DNA, Neoplasm; D006454:Hemoglobins; D016898:Interferon-alpha; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D012261:Ribonucleoproteins; D005914:Globins