Regulation of the androgen receptors in the harderian gland of the male Syrian hamster: influence of photoperiod, castration, and chronic melatonin treatment.
Male Syrian hamsters that were exposed for 8 weeks to short photoperiod (LD 10:14) or treated with melatonin in the late afternoon under long photoperiod conditions (LD 14:10) had a significantly higher content of androgen receptors in the Lipidex-purified soluble fractions isolated from the Harderian glands as compared to the long photoperiod (LD 14:10) exposed controls. Simultaneous computer-assisted analyses of all series of saturation and competition experiments revealed that the numerical value of the apparent Kd, as determined by using the synthetic androgen R-1881 (methyltrienolone), was not different between the experimental groups, and ranged from 0.050 to 0.067 nM. Of the principal natural androgens, testosterone (T) was most potent in inhibiting methyltrienolone binding to the receptor (Ki values from 0.33 to 0.55 nM), and 5 alpha-dihydrotestosterone (DHT) and delta 4-androstenedione (AD) were less effective (Ki values between 1 and 1.9 nM). In the hypothalami and pituitaries of the same animals, used in parallel control assays, DHT was twice as potent as T. Short-term castration (24 hr post-orchidectomy) did not result in significant changes in the receptor binding characteristics. Following 8 weeks exposure to a long photoperiod (LD 14:10) the Bmax values demonstrated a four-fold increase in castrated animals (179 fmoles/mg protein vs. 47 fmoles/mg protein) over intact controls. The relative binding affinity of the major androgens under these conditions remained unchanged, with the exception of AD, where a five-fold increase in the numerical Ki values (decrease in the binding affinity) was recorded (Ki = 9.6 nM).(ABSTRACT TRUNCATED AT 250 WORDS)
Stankov
B
B
Chair of Chemotherapy, University of Milan, Italy.
Lucini
V
V
Negri-Cesi
P
P
Cozzi
B
B
Fumagalli
P
P
Fraschini
F
F
D000818:Animals; D001667:Binding, Competitive; D006224:Cricetinae; D006243:Harderian Gland / Q000378:metabolism*; D007031:Hypothalamus / Q000378:metabolism; D008297:Male; D008550:Melatonin / Q000502:physiology*; D008647:Mesocricetus / Q000502:physiology*; D009919:Orchiectomy; D009929:Organ Size; D017440:Photoperiod; D010902:Pituitary Gland / Q000378:metabolism; D011944:Receptors, Androgen / Q000378:metabolism*; D013737:Testis / Q000502:physiology*
D011944:Receptors, Androgen; D008550:Melatonin