title: Identification of functionally active fragments of staphylococcal enterotoxin B.
abstract: It has been found that staphylococcal enterotoxin B contains a proteolysis-sensitive sequence in the cysteine loop formed by two half-cystines located in the middle of the toxin polypeptide chain. Fragments of the enterotoxin formed as a result of its digestion in this region have been isolated, their N-terminal sequences have been determined and sites of proteolysis have been identified. It has been demonstrated that the N-terminal fragment of staphylococcal enterotoxin B is capable of activating T cell proliferation in the culture of human mononuclear cells practically to the same degree as the intact enterotoxin. The toxin's C-terminal fragment possesses an ability to activate calmodulin-dependent enzymes and is probably the toxicogenic part of the enterotoxin.
authors.affiliation: Research Center of Molecular Diagnostics and Therapy, Moscow, Russia.
authors.lastname: Alakhov VYu
authors.lastname: Klinsky EYu
authors.forename: M I
authors.initials: MI
authors.lastname: Kolosov
authors.forename: I
authors.initials: I
authors.lastname: Maurer-Fogy
authors.lastname: Moskaleva EYu
authors.forename: P G
authors.initials: PG
authors.lastname: Sveshnikov
authors.forename: L P
authors.initials: LP
authors.lastname: Pozdnyakova
authors.forename: O B
authors.initials: OB
authors.lastname: Shemchukova
authors.forename: E S
authors.initials: ES
authors.lastname: Severin
mesh_terms: D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D004768:Enterotoxins / Q000737:chemistry* / Q000494:pharmacology; D006801:Humans; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry; D012689:Sequence Homology, Nucleic Acid; D013211:Staphylococcus aureus / Q000378:metabolism*; D013601:T-Lymphocytes / Q000166:cytology / Q000187:drug effects
chemical_list: D000911:Antibodies, Monoclonal; D004768:Enterotoxins; D010446:Peptide Fragments; C031818:enterotoxin B, staphylococcal