title: Chiral separations of basic drugs and quantitation of bupivacaine enantiomers in serum by capillary electrophoresis with modified cyclodextrin buffers.
abstract: Modified cyclodextrin derivatives were evaluated as the buffer additives in capillary electrophoresis of several racemic pharmaceutical bases. Uncoated and polyacrylamide-modified silica capillaries were compared for their effectiveness in the enantiomeric resolution and migration reproducibility of model solutes. Using cationic detergents in the mixed-micellar mode, optimized separations of the racemic drug bupivacaine are demonstrated in a spiked serum sample at the therapeutic level. Precision, linearity and sensitivity of the method appear adequate for reliable quantitation required in pharmacokinetic and clinical studies.
authors.affiliation: Department of Chemistry, Indiana University, Bloomington 47405.
authors.forename: H
authors.initials: H
authors.lastname: Soini
authors.forename: M L
authors.initials: ML
authors.lastname: Riekkola
authors.forename: M V
authors.initials: MV
authors.lastname: Novotny
mesh_terms: D002021:Buffers; D002045:Bupivacaine / Q000097:blood*; D002482:Cellulose; D003505:Cyclodextrins; D003902:Detergents; D004586:Electrophoresis; D004591:Electrophoresis, Polyacrylamide Gel; D005473:Fluoxetine / Q000097:blood; D006801:Humans; D008619:Mepivacaine / Q000097:blood; D004364:Pharmaceutical Preparations / Q000302:isolation & purification*; D013237:Stereoisomerism; D014700:Verapamil / Q000097:blood
chemical_list: D002021:Buffers; D003505:Cyclodextrins; D003902:Detergents; D004364:Pharmaceutical Preparations; D005473:Fluoxetine; D002482:Cellulose; D008619:Mepivacaine; D014700:Verapamil; D002045:Bupivacaine