Protective effects of coenzyme Q10 on ischemia-induced reperfusion injury in ischemic limb models.
We examined simple cold preservation of rat limbs in Euro-Collins' solution to elucidate the protective effect of coenzyme Q10 (CoQ10) on the ischemia-induced reperfusion injury in an ischemic extremity replant model. A total of 126 Lewis rat limb replants were performed. Limbs were amputated from donor rats and preserved at 4 degrees C in Euro-Collins' solution and were orthotopically grafted to isogeneic rats by microsurgical technique. In the experimental groups (n = 42), coenzyme Q10 (10 mg/kg) was injected intraperitoneally into the recipients about 1 hour before reperfusion. In the control groups (n = 84), the same dose of solvent was given by the same route. We evaluated vascular patency of anastomoses by direct observation or microangiogram and performed histologic examinations 7 days after replantation. In the control groups, the ischemic limit was 96 hours. Ischemic limbs treated with coenzyme Q10 showed a statistically significant (p < 0.05) improvement in vascular patency after 72 and 96 hours of ischemia. Histologically, bone viability with osteoblastic activity was maintained in coenzyme Q10-treated animals of the 72-hour ischemic group. We conclude that the protective effect of coenzyme Q10 on reperfusion injury is suggested in this replant model.
Yokoyama
K
K
Department of Orthopedic Surgery, Kitasato University School of Medicine, Kanagawa, Japan.
Itoman
M
M
Takagishi
K
K
Yamamoto
M
M
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D003067:Coenzymes; D003080:Cold Temperature; D006614:Hindlimb / Q000098:blood supply / Q000637:transplantation; D006982:Hypertonic Solutions; D008297:Male; D051381:Rats; D011917:Rats, Inbred Lew; D015427:Reperfusion Injury / Q000473:pathology / Q000517:prevention & control*; D013997:Time Factors; D014021:Tissue Preservation; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000494:pharmacology
D000975:Antioxidants; D003067:Coenzymes; C037096:Euro-Collins' solution; D006982:Hypertonic Solutions; D014451:Ubiquinone; C024989:coenzyme Q10