Immobilization of alpha-amylase into photographic gelatin by chemical cross-linking.
alpha-Amylase was immobilized into photographic gelatin by chemical cross-linking with chromium (III) acetate and chromium (III) sulphate. Cellulose triacetate film strips, enabled simple handling when coated with an alpha-amylase-gelatin mixture, accomplishing a high degree of durability during consecutive immersions into reaction media. The optimum conditions for pH, substrate concentration, temperature, incubation time and storing conditions of free and immobilized alpha-amylase were determined. The effect of use number on activity and the effect of resting on reuse number were also determined. Photographic gelatin was found to be a very efficient natural polymer, due to its extraordinary diffusion characteristics for immobilization as a carrier.
Bayramoglu
Z
Z
Department of Chemistry, Middle East Technical University, Ankara, Turkey.
Akbulut
U
U
Sungur
S
S
D003432:Cross-Linking Reagents; D004800:Enzymes, Immobilized / Q000378:metabolism*; D005780:Gelatin; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D010781:Photography; D012995:Solubility; D013696:Temperature; D013997:Time Factors; D000516:alpha-Amylases / Q000378:metabolism*
D003432:Cross-Linking Reagents; D004800:Enzymes, Immobilized; D005780:Gelatin; D000516:alpha-Amylases