[The use of the vif gene expression product of HIV-1 in bacteria for detecting specific antibodies in the sera of infected persons].
A fragment of the genome of human immunodeficiency virus type 1 (HIV-1) coding for p23 protein, the product of vif gene, was cloned in a plasmid vector pUR291. The resulting recombinant plasmid pLacVif1 was conducive in E. coli cells to the synthesis of a hybrid polypeptide with molecular weight of 136 kDa containing antigenic determinants of p23 protein of HIV-1. The employment of this polypeptide for analysis of HIV-1-positive sera by indirect enzyme immunoassay showed that vif-specific antibodies were found in 53% of the cases and their appearance was not related to the stage of the disease.
Zverev
S Ia
SIa
Bobkov
A F
AF
Garaev
M M
MM
D000918:Antibody Specificity; D003001:Cloning, Molecular / Q000379:methods; D004926:Escherichia coli / Q000235:genetics*; D016346:Gene Products, vif / Q000032:analysis / Q000235:genetics; D015483:HIV Antibodies / Q000097:blood*; D006679:HIV Seropositivity / Q000175:diagnosis; D015497:HIV-1 / Q000235:genetics / Q000276:immunology*; D006801:Humans; D007124:Immunoenzyme Techniques; D008970:Molecular Weight; D010957:Plasmids / Q000235:genetics; D011994:Recombinant Proteins / Q000032:analysis / Q000235:genetics; D054320:vif Gene Products, Human Immunodeficiency Virus
D016346:Gene Products, vif; D015483:HIV Antibodies; D011994:Recombinant Proteins; D054320:vif Gene Products, Human Immunodeficiency Virus
Ispol'zovanie produkta ékspressii gena vif VICh-1 v bakteriiakh dlia obnaruzeniia spetsificheskikh antitel v syvorotkakh infitsirovannykh.