Benastatins A and B, new inhibitors of glutathione S-transferase, produced by Streptomyces sp. MI384-DF12. I. Taxonomy, production, isolation, physico-chemical properties and biological activities.
Benastatins have been isolated as part of a program designed to find microorganism-produced inhibitors of glutathione S-transferase from Streptomyces sp. MI384-DF12. They were purified by chromatography of reversed-phase silica gel, silica gel and Capcell Pak C18 (HPLC) followed by solvent extraction and then isolated as yellow powders. Benastatins A and B have the molecular formulae, C30H28O7 and C30H30O7, respectively. They were competitive with 3,4-dichloronitrobenzene as the substrate, and the inhibition constants (Ki) of benastatins A and B were 5.0 x 10(-6) and 3.7 x 10(-6), respectively.
Aoyagi
T
T
Institute of Microbial Chemistry, Tokyo, Japan.
Aoyama
T
T
Kojima
F
F
Matsuda
N
N
Maruyama
M
M
Hamada
M
M
Takeuchi
T
T
D000818:Animals; D001551:Benz(a)Anthracenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D005982:Glutathione Transferase / Q000037:antagonists & inhibitors*; D006094:Gram-Positive Bacteria / Q000187:drug effects; D051379:Mice; D008826:Microbial Sensitivity Tests; D009279:Naphthacenes; D051381:Rats; D013302:Streptomyces / Q000737:chemistry* / Q000235:genetics
D001551:Benz(a)Anthracenes; D004791:Enzyme Inhibitors; D009279:Naphthacenes; C077629:benastatin A; C077630:benastatin B; D005982:Glutathione Transferase