title: Attachment of a single fluorescent label to peptides for determination by capillary zone electrophoresis.
abstract: Complicated electropherograms are produced in the separation of fluorescently labeled peptides. Incomplete labeling of epsilon-amino groups on lysine residues results in the production of 2n-1 reaction products, where n is the number of alpha and epsilon amino groups in the peptide. A single label is attached to the peptide by first taking the peptide through one cycle of the Edman degradation reaction. All epsilon-amino groups are converted to the phenyl thiocarbamyl and the cleavage step exposes one alpha-amino group at the N-terminus of the peptide; the fluorescent label is attached to the N-terminus.
authors.affiliation: Department of Chemistry, University of Alberta, Edmonton, Canada.
authors.forename: J Y
authors.initials: JY
authors.lastname: Zhao
authors.forename: K C
authors.initials: KC
authors.lastname: Waldron
authors.forename: J
authors.initials: J
authors.lastname: Miller
authors.forename: J Z
authors.initials: JZ
authors.lastname: Zhang
authors.forename: H
authors.initials: H
authors.lastname: Harke
authors.forename: N J
authors.initials: NJ
authors.lastname: Dovichi
mesh_terms: D000595:Amino Acid Sequence; D004586:Electrophoresis; D005456:Fluorescent Dyes; D007202:Indicators and Reagents; D008969:Molecular Sequence Data; D010455:Peptides / Q000032:analysis*; D013050:Spectrometry, Fluorescence
chemical_list: D005456:Fluorescent Dyes; D007202:Indicators and Reagents; D010455:Peptides