Application of two-color immunofluorescence staining to demonstration of T-cells and HLA-DR-bearing cells in rheumatoid synovitis.
We studied the localization of T-cells and HLA-DR antigen-bearing (DR+) cells in rheumatoid synovitis by employing an improved two-color immunofluorescent staining (TCIF) technique. With this technique we have successfully identified DR+ activated T-cells in the inflammatory synovium. T-cells expressed HLA-DR antigen when they were in contact with DR+ antigen-presenting cells (APC). In addition, activated T-cells showed characteristic distribution within the synovium: they were found around high endothelial venules, within lymphoid follicles, and in hyperplastic synovial lining, suggesting their involvement in the development of rheumatoid synovial lesions via interaction with synovial DR+ APC lineage cells. These findings may contribute to better understanding of the role of activated T-cells in the histogenesis of rheumatoid synovitis, a typical chronic inflammatory lesion.
Itoh
J
J
Laboratory of Biology, Nippon Kayaku Co., Ltd., Tokyo, Japan.
Kinjoh
K
K
Ohyama
A
A
Nose
M
M
Kyogoku
M
M
D001172:Arthritis, Rheumatoid / Q000378:metabolism* / Q000473:pathology; D005455:Fluorescent Antibody Technique; D006684:HLA-DR Antigens / Q000378:metabolism*; D006801:Humans; D006965:Hyperplasia; D016253:Microscopy, Immunoelectron; D013583:Synovial Membrane / Q000166:cytology; D013585:Synovitis / Q000378:metabolism* / Q000473:pathology; D013601:T-Lymphocytes / Q000378:metabolism*
D006684:HLA-DR Antigens