[The technology for isolating the avian myeloblastosis virus in high titers from leukosis-free chicks].
In order to enhance the outcome of high-quality reverse transcriptase enzyme, an efficient biotechnology was developed of accumulating and isolating the avian myeloblastosis virus (AMV) in high titres from blood plasma of leukosis-free chickens. When commercial chickens are infected in most sensitive one-day age, the virus titre does not exceed the value of 10(12) particles per 1 ml of plasma. We used 3-4-day old leukosis free chickens and achieved a stable average titre of the virus of 5.10(12) particles/ml due to adaptation of the virus to such chickens and their selection for a high sensitivity to AMV.
Rat'kin
E V
EV
Piker
E G
EG
Zlotina
E V
EV
D000818:Animals; D001353:Avian Leukosis / Q000382:microbiology*; D009189:Avian Myeloblastosis Virus / Q000302:isolation & purification*; D002645:Chickens / Q000382:microbiology*; D012692:Serial Passage; D013997:Time Factors; D014766:Viremia / Q000382:microbiology* / Q000662:veterinary; D014773:Virology / Q000379:methods
Tekhnologiia polucheniia virusa mieloblastoza ptits v vysokikh titrakh na bezleĭkoznykh tsypliatakh.