Studies on virulence determinants of Plesiomonas shigelloides.
Virulence determinants of nineteen strains of Plesiomonas shigelloides isolated from stool samples of diarrhoeal children were studied. Heat-labile toxin was detected in seven strains using rat ileal loop model and in none of the strains using Chinese hamster ovary cell assay system and by Enzyme-linked immunosorbent assay. Rat ileal loop model was used for the first time to assay toxin in P. shigelloides and is suggested to be a cheap and effective method of detecting labile toxin in the organism. Heat stable toxin, cytotoxin, hemolysin and hemagglutinin were not detected in the strains tested. Invasiveness could not be established by using HEp-2 cell assay system. The results of this study provide some experimental support for an etiological role for P. shigelloides in the production of diarrhoea.
Amin
I I
II
Department of Microbiology, Institute of Post-Graduate Medicine and Research, Dhaka.
Hossain
M A
MA
Hossain
M
M
Miah
M R
MR
Rahman
Z
Z
Rahman
K M
KM
D000818:Animals; D002460:Cell Line; D003967:Diarrhea / Q000382:microbiology; D004768:Enterotoxins / Q000096:biosynthesis; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D016981:Plesiomonas / Q000302:isolation & purification / Q000378:metabolism / Q000472:pathogenicity*; D051381:Rats; D014774:Virulence
D004768:Enterotoxins