title: Protein-coding introns from the 23S rRNA-encoding gene form stable circles in the hyperthermophilic archaeon Pyrobaculum organotrophum.
abstract: Two archaeal introns have been discovered in the single-copy 23S rRNA-encoding gene of the hyperthermophile, Pyrobaculum organotrophum. After excision from rRNA transcripts, both introns circularize and are stably retained in the cell. Putative proteins encoded by the introns and covering most of the intron sequence share a decapeptide motif with proteins encoded by another archaeal intron and by group I introns.
authors.affiliation: Institute of Biological Chemistry B, Copenhagen University, Denmark.
authors.forename: J Z
authors.initials: JZ
authors.lastname: Dalgaard
authors.forename: R A
authors.initials: RA
authors.lastname: Garrett
mesh_terms: D000595:Amino Acid Sequence; D001105:Archaea / Q000235:genetics*; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics; D001483:Base Sequence; D015139:Blotting, Southern; D005798:Genes, Bacterial; D007438:Introns; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D016133:Polymerase Chain Reaction; D017433:Protein Structure, Secondary; D012326:RNA Splicing; D012338:RNA, Ribosomal, 23S / Q000235:genetics*; D012269:Ribosomal Proteins / Q000737:chemistry / Q000235:genetics; D017386:Sequence Homology, Amino Acid
chemical_list: D001426:Bacterial Proteins; D012338:RNA, Ribosomal, 23S; D012269:Ribosomal Proteins