title: Metabolism of arachidonic acid by isolated rabbit ciliary epithelium.
abstract: We examined the ability of rabbit ciliary epithelium to metabolize arachidonic acid in vitro. The epithelium was homogenized and incubated with 14C-labeled arachidonic acid. 14C-labeled metabolites were extracted and then separated by thin layer chromatography. The range of arachidonic acid metabolites synthesized by ciliary epithelium was compared to the metabolites generated by rabbit iris-ciliary body. Ciliary epithelium produced substantial amounts of arachidonic acid metabolites that comigrated with 5-HETE and 12-HETE. Authenticity of the 12-HETE produced by ciliary epithelium was confirmed by gas chromatography/mass spectrometry. The ciliary epithelium generated only small amounts of the cyclooxygenase products, PGF2 alpha, PGE2, PGD2 and 6k-PGF1 alpha. In contrast, the iris-ciliary body produced large amounts of cyclooxygenase products such as PGF2 alpha and PGD2. The ability of the ciliary epithelium to generate 12-HETE is noteworthy since 12(R)-HETE is known to be capable of lowering intraocular pressure.
authors.affiliation: Department of Ophthalmology and Visual Sciences, Kentucky Lions Eye Research Institute, Louisville.
authors.forename: K L
authors.initials: KL
authors.lastname: King
authors.forename: N A
authors.initials: NA
authors.lastname: Delamere
authors.forename: S C
authors.initials: SC
authors.lastname: Csukas
authors.forename: W M
authors.initials: WM
authors.lastname: Pierce
mesh_terms: D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism*; D002855:Chromatography, Thin Layer; D002924:Ciliary Body / Q000378:metabolism*; D015232:Dinoprostone / Q000378:metabolism; D004848:Epithelium / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry; D006893:Hydroxyeicosatetraenoic Acids / Q000378:metabolism; D066298:In Vitro Techniques; D015230:Prostaglandin D2 / Q000378:metabolism; D011817:Rabbits; D014602:Uvea / Q000378:metabolism
chemical_list: D006893:Hydroxyeicosatetraenoic Acids; D016718:Arachidonic Acid; D015232:Dinoprostone; D015230:Prostaglandin D2