title: Both purified human 1,N6-ethenoadenine-binding protein and purified human 3-methyladenine-DNA glycosylase act on 1,N6-ethenoadenine and 3-methyladenine.
abstract: We previously described a protein, isolated from human tissues and cells, that bound to a defined double-stranded oligonucleotide containing a single site-specifically placed 1,N6-ethenoadenine. It was further demonstrated that this protein was a glycosylase and released 1,N6-ethenoadenine. We now find that this enzyme also releases 3-methyladenine from methylated DNA and that 3-methyladenine-DNA glycosylase behaves in the same manner, binding to the ethenoadenine-containing oligonucleotide and cleaving both ethenoadenine and 3-methyladenine from DNA containing these adducts. The rate and extent of glycosylase activities toward the two adducts are similar.
authors.affiliation: Donner Laboratory, Life Sciences Division, Lawrence Berkeley Laboratory, University of California, Berkeley 94720.
authors.forename: B
authors.initials: B
authors.lastname: Singer
authors.forename: A
authors.initials: A
authors.lastname: Antoccia
authors.forename: A K
authors.initials: AK
authors.lastname: Basu
authors.forename: M K
authors.initials: MK
authors.lastname: Dosanjh
authors.forename: H
authors.initials: H
authors.lastname: Fraenkel-Conrat
authors.forename: P E
authors.initials: PE
authors.lastname: Gallagher
authors.forename: J T
authors.initials: JT
authors.lastname: Kuśmierek
authors.forename: Z H
authors.initials: ZH
authors.lastname: Qiu
authors.forename: B
authors.initials: B
authors.lastname: Rydberg
mesh_terms: D000225:Adenine / Q000031:analogs & derivatives* / Q000378:metabolism; D045647:DNA Glycosylases; D004260:DNA Repair; D004268:DNA-Binding Proteins / Q000302:isolation & purification / Q000378:metabolism*; D006801:Humans; D009699:N-Glycosyl Hydrolases / Q000302:isolation & purification / Q000378:metabolism*; D010920:Placenta / Q000201:enzymology; D013379:Substrate Specificity
chemical_list: D004268:DNA-Binding Proteins; C004981:1,N(6)-ethenoadenine; C025946:3-methyladenine; C021565:3-methyladenine-DNA glycosylase; D045647:DNA Glycosylases; D009699:N-Glycosyl Hydrolases; D000225:Adenine