[Quantitative characteristics of interactions of blood lipoproteins and steroid hormones].
Human and serum lipoproteins interaction with steroid hormones (corticosterone and hydrocortisone) were studied. Methods of fluorescence quenching titration and equilibrium dialysis were used for quantitative evaluation of VLDL, LDL and HDL glucocorticoids binding ability. Association constants were found to be 0.6-2.0 x 10 M for corticosterone and 4.0-8.0 x 10 M for hydrocortisone. The number of binding sites ranged from 3 to 300 for different classes of lipoproteins. Our data suggest high specificity of serum lipoproteins binding with corticosterone and hydrocortisone.
Panin
L E
LE
Biushkina
N G
NG
Poliakov
L M
LM
D000818:Animals; D001665:Binding Sites; D002852:Chromatography, Ion Exchange; D003345:Corticosterone / Q000097:blood* / Q000378:metabolism; D006801:Humans; D006854:Hydrocortisone / Q000097:blood* / Q000378:metabolism; D008074:Lipoproteins / Q000097:blood* / Q000378:metabolism; D008075:Lipoproteins, HDL / Q000097:blood / Q000378:metabolism; D008077:Lipoproteins, LDL / Q000097:blood / Q000378:metabolism; D008079:Lipoproteins, VLDL / Q000097:blood / Q000378:metabolism; D051381:Rats; D013050:Spectrometry, Fluorescence
D008074:Lipoproteins; D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL; D008079:Lipoproteins, VLDL; D003345:Corticosterone; D006854:Hydrocortisone
Kolichestvennaia kharakteristika vzaimodeĭstviia lipoproteinov syvorotki krovi so steroidnymi gormonami.