Integration of image analysis and robotics into a fully automated colony picking and plate handling system.
We describe here the integration of image analysis and robotics to produce a fully automated colony picking/plate handling system. Biological tests were performed to verify its performance in terms of sterilisation and accuracy of picking. The machine was then used by a single operative to pick a 36,000 clone cDNA library in approximately 42 hrs over 5 days.
Jones
P
P
MRC Laboratory of Molecular Biology, MRC Centre, Cambridge, UK.
Watson
A
A
Davies
M
M
Stubbings
S
S
D001331:Automation / Q000295:instrumentation*; D010582:Bacteriophage lambda / Q000235:genetics; D003001:Cloning, Molecular; D015169:Colony Count, Microbial / Q000295:instrumentation*; D004926:Escherichia coli / Q000235:genetics; D015723:Gene Library; D007091:Image Processing, Computer-Assisted / Q000295:instrumentation; D016133:Polymerase Chain Reaction; D012371:Robotics / Q000295:instrumentation