[An analysis of the DNA content in a stage-T-1 epidermoid carcinoma of the vocal cord. The methodology and results].
DNA content of tumor cells determined by flow cytometry has been correlated to biologic behavior and to prognosis for many solid tumors, but insufficient data has been accumulated for squamous cell carcinoma of the larynx. In order to know the predictive value of this parameter in the prognosis of T-1 vocal cord squamous cell carcinoma, we have studied 27 patients submitted to cordectomy. The patients outcome was followed for a minimum of five years or until death, DNA content was determined by Hedley method in paraffin-embedded sections of formalin-fixed blocks in an EPICS Profile flow cytometer. 24 of these tumors were diploid (89%) and 3 aneuploid (11%). We didn't find statistical correlation between ploidy and survival, local failure or someone of the pathological parameters.
Barona
R
R
Servicio de ORL, Hospital General Universitario, Universidad de Valencia.
Martorell
M A
MA
Basterra
J
J
Montoro
A
A
Montoro
J
J
Garín
L
L
Armengot
M
M
D002294:Carcinoma, Squamous Cell / Q000737:chemistry* / Q000453:epidemiology / Q000473:pathology; D016009:Chi-Square Distribution; D004273:DNA, Neoplasm / Q000032:analysis*; D005434:Flow Cytometry / Q000295:instrumentation / Q000379:methods / Q000706:statistics & numerical data; D006801:Humans; D007822:Laryngeal Neoplasms / Q000737:chemistry* / Q000453:epidemiology / Q000473:pathology; D009367:Neoplasm Staging; D014827:Vocal Cords / Q000473:pathology
D004273:DNA, Neoplasm
Análisis del contenido de DNA en el carcinoma epidermoide de cuerda vocal en estadio T-1. Metodología y resultados.