A simple and rapid method for the preparation of apolipoproteins for electrophoresis.
A new method is described for the rapid preparation of apolipoproteins for polyacrylamide gel electrophoresis. It is suitable for all serum lipoproteins including chylomicrons. The procedure involves extraction with diethyl ether in the presence of trichloroacetic acid and sodium deoxycholate. The method gives an improved protein recovery, in particular with chylomicrons. In addition, samples do not require dialysis to remove salts (e.g., KBr) prior to processing; as a consequence, the procedure requires only 1 h. Due to this rapidity and the high yields, the procedure is superior to present methods utilizing ethanol-diethyl ether extraction.
Mindham
M A
MA
Department of Veterinary Basic Sciences, Royal Veterinary College (University of London), United Kingdom.
Mayes
P A
PA
D000818:Animals; D001053:Apolipoproteins / Q000032:analysis*; D003840:Deoxycholic Acid; D004591:Electrophoresis, Polyacrylamide Gel; D000431:Ethanol; D004986:Ether; D006801:Humans; D008722:Methods; D051381:Rats; D013997:Time Factors; D014238:Trichloroacetic Acid
D001053:Apolipoproteins; D003840:Deoxycholic Acid; D004986:Ether; D000431:Ethanol; D014238:Trichloroacetic Acid