title: Modifications of adhesion properties and proteoglycan structure in rat embryo fibroblast cultures with increasing passages.
abstract: Adhesion properties of rat embryo fibroblast cultures and proteoglycans (PGs) produced both in the growth medium and in the cell layer were investigated with increasing passages. Both cell-cell and cell-substrate adhesion increased with increasing subculture number. Cell adhesion properties were improved by cell treatment with chondroitinase ABC. The increase in subculture number was coupled with a constant increase of PG molecular size, which was particularly evident in cell layer extracts. The ratio HS-PGs/DS-PGs increased with increasing passages. PG modifications are likely to represent evidence of changes in extracellular matrix organization and could play a role in the increase of cell adhesion properties.
authors.affiliation: Institute of General Physiology and Biological Chemistry, University of Sassari, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Pippia
authors.forename: M
authors.initials: M
authors.lastname: Formato
authors.forename: L
authors.initials: L
authors.lastname: Sciola
authors.forename: G
authors.initials: G
authors.lastname: Tilloca
authors.forename: A
authors.initials: A
authors.lastname: Bardoni
authors.forename: G
authors.initials: G
authors.lastname: Pallavicini
authors.forename: A
authors.initials: A
authors.lastname: Passi
authors.forename: S
authors.initials: S
authors.lastname: Rindi
authors.forename: R
authors.initials: R
authors.lastname: Salvini
authors.forename: G
authors.initials: G
authors.lastname: De Luca
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D002450:Cell Communication; D002478:Cells, Cultured; D004622:Embryo, Mammalian / Q000166:cytology; D005109:Extracellular Matrix / Q000737:chemistry / Q000502:physiology; D005347:Fibroblasts / Q000737:chemistry / Q000187:drug effects / Q000502:physiology*; D007700:Kinetics; D011509:Proteoglycans / Q000032:analysis / Q000737:chemistry*; D051381:Rats
chemical_list: D011509:Proteoglycans