Low and marginal copper intake by postweanling rats: effects on copper status and resistance to carbon tetrachloride hepatotoxicity.
Copper feeding studies in rats are generally initiated at weaning. This study examined whether a 6-week feeding of low or marginal Cu levels (0.2 or 2.5 ppm) to rats initially weighing 135 g produced deleterious effects. Controls were fed 8 ppm Cu. Liver Cu-Zn superoxide dismutase activities paralleled Cu intake. Plasma ceruloplasmin activities were very low for both low and marginal Cu consumption. Low but not marginal Cu intake caused a low body weight, high plasma cholesterol level, anemia, cardiac hypertrophy, and a high degree of hepatic plasma membrane injury 24 hours after CCl4 injection (150 microL/kg intraperitoneally [IP]). In summary, low and marginal Cu intakes produced low Cu enzyme activities, while low Cu intake produced pathological symptoms and poor resistance to an oxidative stress.
DiSilvestro
R A
RA
Department of Human Nutrition and Food Management, Ohio State University, Columbus 43210.
Medeiros
D M
DM
D000284:Administration, Oral; D000818:Animals; D001692:Biological Transport / Q000502:physiology; D002252:Carbon Tetrachloride Poisoning / Q000150:complications; D002570:Ceruloplasmin / Q000032:analysis; D056486:Chemical and Drug Induced Liver Injury; D002784:Cholesterol / Q000097:blood; D003300:Copper / Q000008:administration & dosage* / Q000172:deficiency / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D004351:Drug Resistance; D005527:Food, Fortified; D007113:Immunity, Innate; D007274:Injections, Intraperitoneal; D008099:Liver / Q000187:drug effects / Q000201:enzymology / Q000473:pathology; D008107:Liver Diseases / Q000276:immunology; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000378:metabolism; D014886:Weaning
D003300:Copper; D002784:Cholesterol; D013482:Superoxide Dismutase; D002570:Ceruloplasmin