[The antigenic and immunogenic properties of a recombinant protein of the HIV-1 gag-pol gene secreted by Saccharomyces cerevisiae yeast cells].
Analysis of the immunological properties of recombinant proteins of HIV-1 gene gag-pol secreted by yeast cells S. cerevisiae was carried out. The proteins under study interacted with antibodies from HIV-1-seropositive human subjects and with antibodies of rabbit immune serum to the native virus as effectively and specifically as natural HIV-1 proteins. The yeast gag-pol-protein complex was markedly immunogenic and induced in animals synthesis of antibodies of a certain specificity spectrum. A comparative immunochemical analysis of the properties of the recombinant proteins carried out by EIA and immune blot showed a certain degree of similarity between the yeast proteins and those of analogous construction produced in E. coli system.
Trushinskaia
G N
GN
Grudinin
M P
MP
Denisova
G F
GF
Gibadulin
R A
RA
D000818:Animals; D000956:Antigens, Viral / Q000097:blood / Q000276:immunology*; D001426:Bacterial Proteins / Q000097:blood / Q000276:immunology; D004926:Escherichia coli / Q000502:physiology; D005656:Fungal Proteins / Q000097:blood / Q000276:immunology / Q000378:metabolism*; D015683:Gene Products, gag / Q000097:blood / Q000276:immunology*; D015684:Gene Products, pol / Q000097:blood / Q000276:immunology*; D015483:HIV Antibodies / Q000097:blood; D015497:HIV-1 / Q000235:genetics / Q000276:immunology*; D006801:Humans; D007114:Immunization / Q000379:methods; D015151:Immunoblotting; D007124:Immunoenzyme Techniques; D011817:Rabbits; D011994:Recombinant Proteins / Q000097:blood / Q000276:immunology; D012441:Saccharomyces cerevisiae / Q000502:physiology*
D000956:Antigens, Viral; D001426:Bacterial Proteins; D005656:Fungal Proteins; D015683:Gene Products, gag; D015684:Gene Products, pol; D015483:HIV Antibodies; D011994:Recombinant Proteins
Antigennye i immunogennye svoĭstva rekombinantnogo belka gena gag-pol VICh-1, sekretiruemogo drozhzhevymi kletkami Saccharomyces cerevisiae.