title: Immunocytochemical localization of Bombyx-PTTH-like molecules in neurosecretory cells of the brain of the migratory locust, Locusta migratoria. A comparison with neuroparsin and insulin-related peptide.
abstract: Using a monoclonal antibody directed against a synthetic pentadecapeptide corresponding to the N-terminus of the prothoracicotropic hormone (PTTH) of Bombyx mori, we report the presence of immunoreactive molecules in a large number of median neurosecretory cells of the pars intercerebralis of the migratory locust, Locusta migratoria. These cells correspond to the A1 cell type which we show to contain also neuroparsins, a family of predominant neurohormones of the migratory locust. In contrast, PTTH-like molecules are absent from A2 cells of the pars intercerebralis which contain Locusta insulin-related peptide (LIRP). Developmental studies show the presence of PTTH-related substances in neurosecretory cells of Locusta migratoria from late embryogenesis to adult development, including ageing vitellogenic female adults.
authors.affiliation: Laboratoire de Biologie Générale de l'Université Louis Pasteur, Unité de Recherche Associée au CNRS 672, Endocrinologie et Immunologie des Insectes, Strasbourg, France.
authors.forename: F
authors.initials: F
authors.lastname: Goltzené
authors.forename: F
authors.initials: F
authors.lastname: Holder
authors.forename: M
authors.initials: M
authors.lastname: Charlet
authors.forename: M
authors.initials: M
authors.lastname: Meister
authors.forename: T
authors.initials: T
authors.lastname: Oka
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D001923:Brain Chemistry; D005260:Female; D006110:Grasshoppers / Q000737:chemistry*; D007150:Immunohistochemistry; D007301:Insect Hormones / Q000032:analysis* / Q000737:chemistry; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000032:analysis*
chemical_list: D000911:Antibodies, Monoclonal; D007301:Insect Hormones; D009479:Neuropeptides; C063879:insulin-related neuropeptide; C058919:neuroparsin A; C009089:prothoracicotropic hormone