Evidence that adhesion of electrically permeabilized platelets to collagen is mediated by guanine nucleotide regulatory proteins.
Adhesion of electrically permeabilized platelets to collagen was found to be essentially independent of free Ca2+ concentration in the medium. Addition of stable GTP analogues increased the proportion of adhering cells about 5-fold. This effect was inhibited by guanosine 5'-[beta-thio]diphosphate, cytochalasin D or monoclonal antibodies to glycoprotein Ia. In contrast, the protein kinase C inhibitor staurosporine had only a small effect on the GTP-analogue-enhanced adhesion of the permeabilized cells to collagen. These results suggest that a guanine nucleotide regulatory (G)-protein is directly linked to the collagen receptor and is involved in the actin-dependent recruitment of additional collagen receptors.
Daniel
J L
JL
Department of Pharmacology, Temple University Medical School, Philadelphia, PA 19140.
Dangelmaier
C
C
Smith
J B
JB
D000470:Alkaloids / Q000494:pharmacology; D000911:Antibodies, Monoclonal; D001792:Blood Platelets / Q000166:cytology*; D002118:Calcium / Q000378:metabolism; D002413:Cations, Divalent; D002448:Cell Adhesion / Q000187:drug effects; D002478:Cells, Cultured; D003094:Collagen / Q000378:metabolism*; D015638:Cytochalasin D / Q000494:pharmacology; D004594:Electrophysiology; D019204:GTP-Binding Proteins / Q000502:physiology*; D006801:Humans; D011493:Protein Kinase C / Q000037:antagonists & inhibitors; D019311:Staurosporine
D000470:Alkaloids; D000911:Antibodies, Monoclonal; D002413:Cations, Divalent; D015638:Cytochalasin D; D003094:Collagen; D011493:Protein Kinase C; D019204:GTP-Binding Proteins; D019311:Staurosporine; D002118:Calcium