[The effect of oxygen free radical scavenger on endothelial cell at ischemic and reperfusion injury].
Although a lot of investigations indicated that free radical scavengers, such as superoxide dismutase (SOD) and catalase, reduced ischemia-reperfusion injury, the precise mechanism including the sites of action are still controversial. The purposes of this study were to investigate the sites of action of SOD and catalase by assessing postischemic cardiac and endothelial cell function. Wistar rats (450-550g) were used as support animals to establish blood perfused circuit. A peristaltic pump controlled the femoral arterial supply at 3.0 ml/min and the venous return to the jugular vein with gravity. Hearts (n = 8/group) from the Wistar rats were perfused (Langendorff model) and paced at 350 beats/min. After assessing baseline functions, hearts were rendered ischemia at 37.5 degrees C for 20 min. Then hearts were reperfused for 35 min, at the end of which left ventricular functions were re-assessed. One group of hearts received a continuous infusion of SOD and catalase during the period of 10 min before ischemia to 20 min after the onset of reperfusion. Left ventricular functions were measured with a left intraventricular balloon. The end-diastolic pressure was fixed at 10 mmHg, which was equivalent to the normal left ventricular end-diastolic pressure. The mean postischemic recovery of the left ventricular end-systolic pressure was increased from 55 +/- 3% in the control group to 67 +/- 2% in the SOD and catalase group (P less than 0.05). Similarly, the postischemic compliance was increased from 1.28 +/- 0.12 (microliters/mmHg) in the control group to 1.61 +/- 0.11 (microliters/mmHg) in the SOD and catalase group (P less than 0.05).(ABSTRACT TRUNCATED AT 250 WORDS)
Imamura
M
M
Second Department of Surgery, Hokkaido University School of Medicine, Sapporo, Japan.
D000818:Animals; D002374:Catalase / Q000627:therapeutic use*; D003971:Diastole; D004730:Endothelium, Vascular / Q000503:physiopathology*; D016166:Free Radical Scavengers; D066298:In Vitro Techniques; D008297:Male; D015428:Myocardial Reperfusion Injury / Q000188:drug therapy* / Q000503:physiopathology; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000627:therapeutic use*; D014655:Vascular Resistance; D016277:Ventricular Function, Left
D016166:Free Radical Scavengers; D002374:Catalase; D013482:Superoxide Dismutase