title: Crystallization and preliminary X-ray investigation of bovine liver mitochondrial aldehyde dehydrogenase.
abstract: Aldehyde dehydrogenase from bovine liver mitochondria has been crystallized using the sitting drop method of vapor diffusion at 22 degrees C. The crystals formed from solutions containing, 40 mM-sodium citrate, 1 mM-NAD+ and 21% to 24% polyethylene glycol 3400 (pH 5.3 to 5.5). X-ray diffraction data collected from these crystals indicate that the crystals belong to the orthorhombic space group P2(1)2(1)2(1) with cell dimensions of a = 153.7 A, b = 159.37 A and c = 101.45 A. The crystals diffract to at least 2.9 A and a tetramer may comprise the asymmetric unit.
authors.affiliation: Department of Biochemistry, Indiana University, School of Medicine, Indianapolis 46202.
authors.forename: T D
authors.initials: TD
authors.lastname: Hurley
authors.forename: H
authors.initials: H
authors.lastname: Weiner
mesh_terms: D000444:Aldehyde Dehydrogenase / Q000737:chemistry*; D000818:Animals; D002417:Cattle; D003460:Crystallization; D008930:Mitochondria, Liver / Q000201:enzymology*; D014961:X-Ray Diffraction
chemical_list: D000444:Aldehyde Dehydrogenase