Patterns of ethanol-induced cell death in the developing nervous system of mice; neural fold states through the time of anterior neural tube closure.
Vital staining and routine histological analyses of mouse embryos 12 h after acute maternal ethanol administration (2.9 g/kg) illustrated that selected neuronal cell populations are killed. At the time of treatment, embryos had 5-15 somite pairs, corresponding to the developmental stages occurring in humans during the fourth week of post-fertilization; i.e. when neural folds are present and neural tube fusion begins. Affected cell populations in embryos having 6-26 somite pairs (up to the stage of anterior neuropore closure) were in discrete locations in the alar and basal plates of the rhombencephalon, in the otic placode/vesicle, and in the regions of the epibranchial placodes, olfactory placodes and trigeminal ganglion. The potential basis for the vulnerability of these cell populations to ethanol-induced cell death is discussed. Our understanding of the scope of ethanol-induced CNS damage is dependent upon further defining ethanol-sensitive cell populations at all stages of CNS development.
Kotch
L E
LE
Department of Cell Biology and Anatomy, School of Medicine, University of North Carolina, Chapel Hill 27599.
Sulik
K K
KK
D000818:Animals; D002147:Calmodulin / Q000378:metabolism; D016923:Cell Death / Q000187:drug effects*; D000431:Ethanol / Q000097:blood / Q000494:pharmacology*; D005260:Female; D051379:Mice; D008810:Mice, Inbred C57BL; D008855:Microscopy, Electron, Scanning; D008970:Molecular Weight; D009420:Nervous System / Q000166:cytology* / Q000196:embryology; D009436:Neural Tube Defects / Q000473:pathology*; D011247:Pregnancy
D002147:Calmodulin; D000431:Ethanol