A testis-expressed Zn finger gene (ZNF76) in human 6p21.3 centromeric to the MHC is closely linked to the human homolog of the t-complex gene tcp-11.
A novel testis-expressed Zn finger gene (ZNF76) was identified by screening cDNA libraries with cosmids derived from 6p21. ZNF76 is a member of the GLI-Krüppel family of DNA binding proteins. It is conserved in mouse where transcription in testis is initiated at Day 20 after birth. The mouse tcp-11 gene is located in the distal inversion of the t-complex and is developmentally regulated in the same manner as ZNF76. The human homolog of tcp-11 was isolated to allow a precise chromosomal localization. By using a combination of somatic cell hybrids, radiation hybrids, metaphase and interphase fluorescent in situ hybridization, and pulsed-field gel electrophoresis, we mapped the two genes to the 6p21.2 to 6p21.3 region and linked them to each other within 300 kb of DNA, approximately 2 Mb centromeric to the major histocompatibility complex.
Ragoussis
J
J
Human Immunogenetics Laboratory, ICRF Laboratories, London, United Kingdom.
Senger
G
G
Mockridge
I
I
Sanseau
P
P
Ruddy
S
S
Dudley
K
K
Sheer
D
D
Trowsdale
J
J
D000595:Amino Acid Sequence; D000818:Animals; D002503:Centromere; D002896:Chromosomes, Human, Pair 6; D016521:Electrophoresis, Gel, Pulsed-Field; D008040:Genetic Linkage; D006801:Humans; D006822:Hybrid Cells; D047908:Intracellular Signaling Peptides and Proteins; D008285:Major Histocompatibility Complex; D008297:Male; D008565:Membrane Proteins; D051379:Mice; D008869:Microtubule-Associated Proteins; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics*; D013737:Testis / Q000378:metabolism*; D044767:Ubiquitin-Protein Ligases; D016335:Zinc Fingers / Q000235:genetics*; D056427:t-Complex Genome Region
D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D008869:Microtubule-Associated Proteins; D009687:Nuclear Proteins; C533399:TCP11 protein, human; C533400:Tcp11 protein, mouse; C116196:PPP1R11 protein, human; D044767:Ubiquitin-Protein Ligases