Comparison of polymerase chain reaction, monoclonal antibody based enzyme immunoassay, and cell culture for detection of Chlamydia trachomatis in genital specimens.
An enzyme immunoassay (EIA; AntigEnz Chlamydia; Northumbria Biologicals, Northumberland, United Kingdom) and a polymerase chain reaction (PCR) assay (Genemed Biotechnologies, San Francisco, CA) were evaluated for the detection of Chlamydia trachomatis in urogenital specimens. Of 324 specimens, 23 were positive by cycloheximide-treated McCoy's cell culture method. Of 23 culture-positive specimens, 20 and 22 were found to be positive by EIA and PCR, respectively. Among 301 culture-negative samples, 4 were found to be positive by both PCR and EIA, 2 were PCR-positive and EIA-negative, and 3 PCR-negative and culture-negative specimens were found to be positive by EIA. In comparing nonculture methods with cell culture technique, combined sensitivities of 87.0% (90.9% in men and 83.3% in women) and 95.6% (90.9% in men and 100% in women), and specificities of 97.7% (99.4% in men and 95.7% in women) and 98.0% (99.4% in men and 96.5% in women) are achieved by EIA and PCR, respectively. The PCR proved to be the more sensitive of these two nonculture methods, and it can be used for the rapid diagnosis of C. trachomatis urogenital infection.
Wu
C H
CH
Department of Medical Research, Taichung Veterans General Hospital, Taiwan, Republic of China.
Lee
M F
MF
Yin
S C
SC
Yang
D M
DM
Cheng
S F
SF
D000911:Antibodies, Monoclonal; D015139:Blotting, Southern; D002478:Cells, Cultured; D002584:Cervix Uteri / Q000382:microbiology; D002690:Chlamydia Infections / Q000175:diagnosis*; D002692:Chlamydia trachomatis; D003429:Cross Reactions; D004247:DNA / Q000032:analysis; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity; D014521:Urethra / Q000382:microbiology
D000911:Antibodies, Monoclonal; D004247:DNA