Ponceau S as a dye for quantitative protein assay. Its use in the presence of Triton X-100.
A Ponceau S reagent with high stability and slight response to pH changes, presence of organic solvents and detergents was prepared. Under these conditions, the reagent was tested to be suitable to quantify, in one-step procedure, different standard proteins. Moreover, this method offers the possibility to measure the protein content in the presence of the detergent Triton X-100.
Gracia
E
E
Departamento de Bioquimica y Biologia Molecular, Facultad de Veterinaria, Universidad de Murcia, Spain.
Fernandez-Belda
F
F
D001391:Azo Compounds / Q000737:chemistry*; D004396:Coloring Agents / Q000737:chemistry*; D003902:Detergents; D006863:Hydrogen-Ion Concentration; D017830:Octoxynol; D011092:Polyethylene Glycols / Q000737:chemistry*; D011506:Proteins / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet
D001391:Azo Compounds; D004396:Coloring Agents; D003902:Detergents; D011506:Proteins; D011092:Polyethylene Glycols; D017830:Octoxynol; C032756:ponceau S