Expression of desiccation-related proteins from the resurrection plant Craterostigma plantagineum in transgenic tobacco.
Three cDNAs encoding desiccation-induced proteins from the resurrection plant Craterostigma plantagineum were each ligated to a triplicated CaMV 35S promoter and a nopaline synthase 3'-flanking region in an Agrobacterium vector and introduced into tobacco. Transgenic plants expressed the encoded Craterostigma proteins at high levels. This did not lead to changes in the phenotype, in the growth habit or in basic photosynthetic parameters. In tobacco, one protein was targeted to the chloroplast stroma which is its normal location in Craterostigma. These desiccation-related proteins are not sufficient per se to increase drought tolerance as measured by ion-leakage tests.
Iturriaga
G
G
Max-Planck-Institut für Züchtungsforschung, Köln, Germany.
Schneider
K
K
Salamini
F
F
Bartels
D
D
D001692:Biological Transport; D001937:Brassica / Q000382:microbiology; D002736:Chloroplasts / Q000378:metabolism; D003890:Desiccation; D004190:Disasters; D017343:Genes, Plant / Q000235:genetics; D005818:Genetic Engineering; D006360:Heat-Shock Proteins / Q000235:genetics*; D009029:Mosaic Viruses / Q000235:genetics; D010641:Phenotype; D010788:Photosynthesis / Q000502:physiology; D010940:Plant Proteins / Q000235:genetics*; D010944:Plants / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000235:genetics; D010947:Plants, Toxic; D011401:Promoter Regions, Genetic / Q000235:genetics; D012231:Rhizobium / Q000235:genetics; D014026:Nicotiana / Q000235:genetics
D006360:Heat-Shock Proteins; D010940:Plant Proteins