title: Evaluation of murine splenic cell type metabolism of benzo[a]pyrene and functionality in vitro following repeated in vivo exposure to benzo[a]pyrene.
abstract: Recent studies have demonstrated that macrophages are the cell types capable of metabolizing benzo[a]pyrene (B(a)P) within the spleens of untreated mice. Since repeated exposure to B(a)P results in immunosuppression and B(a)P is known to induce cytochrome P450 levels, the first objective of this study was to investigate whether exposure of mice to B(a)P could increase the amounts of immunosuppressive B(a)P metabolites generated and/or alter the pattern of B(a)P metabolites formed by several different splenic cell types. Mice were dosed with a daily sc dose of 200 mg/kg B(a)P or vehicle for 4 days. Separation of splenocytes based on density by centrifugation through discontinuous Percoll gradients along with immunomagnetic negative selection or antibody-mediated complement lysis was used to obtain different splenic cell populations. Cells were incubated with [3H]B(a)P for 24 hr. High-pressure liquid chromatography was used to separate and quantitate B(a)P metabolites. Results indicate that splenic macrophages of B(a)P-treated mice produced significantly greater amounts of some metabolites compared to those of vehicle-treated mice. The three major metabolites produced were an unidentified peak of polar metabolites containing polyhydroxylated metabolites, B(a)P-9,10- and B(a)P-7,8-dihydrodiols. Other splenic cell types examined did not produce metabolite amounts significantly above (T-cells, PMNs, or the capsule) or just above (B-cells) background. The second objective was to investigate the splenic cell type(s) targeted by B(a)P resulting in suppression of humoral immunity. Separation-reconstitution studies along with in vitro sensitization techniques with several different antigens (sheep red blood cells (SRBC), dinitrophenyl-Ficoll (DNP-Ficoll), lipopolysaccharide (LPS)) were used to identify splenic target cells following exposure of mice to B(a)P (200 mg/kg/day, sc for 4 days). Findings indicate that in vitro plaque-forming cell (PFC) suppression was due to alterations in the adherent (macrophage) cell population. Exposure also suppressed the PFC response to the T-dependent antigen SRBC and the T-independent antigen DNP-Ficoll, but did not suppress the PFC response to the polyclonal antigen, LPS. These data suggest that B(a)P is targeting macrophages.
authors.affiliation: Department of Pharmacology and Toxicology, Medical College of Virginia, Virginia Commonwealth University, Richmond 23298.
authors.forename: G S
authors.initials: GS
authors.lastname: Ladics
authors.forename: T T
authors.initials: TT
authors.lastname: Kawabata
authors.forename: A E
authors.initials: AE
authors.lastname: Munson
authors.forename: K L
authors.initials: KL
authors.lastname: White
mesh_terms: D000818:Animals; D000917:Antibody Formation / Q000187:drug effects / Q000276:immunology; D001402:B-Lymphocytes / Q000166:cytology / Q000378:metabolism; D001564:Benzo(a)pyrene / Q000378:metabolism* / Q000494:pharmacology; D002452:Cell Count; D002469:Cell Separation; D002851:Chromatography, High Pressure Liquid; D004912:Erythrocytes / Q000276:immunology; D005069:Evaluation Studies as Topic; D005260:Female; D005362:Ficoll / Q000031:analogs & derivatives / Q000494:pharmacology; D006462:Hemolytic Plaque Technique; D007165:Immunosuppression Therapy; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000166:cytology / Q000378:metabolism; D051379:Mice; D008815:Mice, Inbred Strains; D009504:Neutrophils / Q000166:cytology / Q000378:metabolism; D012756:Sheep; D013154:Spleen / Q000166:cytology / Q000378:metabolism* / Q000502:physiology; D013601:T-Lymphocytes / Q000166:cytology / Q000378:metabolism; D014316:Tritium
chemical_list: C031007:DNP-Ficoll; D008070:Lipopolysaccharides; D014316:Tritium; D005362:Ficoll; D001564:Benzo(a)pyrene