title: Platelet-activating factor acetylhydrolase activity in red blood cell-stroma from patients with cerebral thrombosis.
abstract: Platelet-activating factor (1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine, PAF) is a bioactive phospholipid and inactivated by a specific enzyme, PAF acetylhydrolase (PAF-AH). We have measured PAF-AH activity in red blood cells (RBCs) from patients with a history of cerebral thrombosis and age-matched healthy controls. The activities in 34 patients and 34 controls were 1.29 +/- 0.28 and 1.64 +/- 0.26 nmol/10(9) RBCs/min (or, 30.0 +/- 5.8 and 35.1 +/- 4.7 nmol/g protein/min) (mean +/- SD), respectively, and the difference was significant (p less than 0.001). In patients, RBC PAF-AH activity correlated positively with RBC filterability, an index of RBC deformability (r = +0.501, p less than 0.05). The RBC activity may play a role in scavenging oxidation products of membrane phospholipids. Lower RBC PAF-AH activity may predispose to ischemic diseases by disturbing microcirculatory behavior of the RBCs.
authors.affiliation: Department of Pathological Physiology, Hirosaki University, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Yoshida
authors.forename: K
authors.initials: K
authors.lastname: Satoh
authors.forename: T
authors.initials: T
authors.lastname: Imaizumi
authors.forename: S
authors.initials: S
authors.lastname: Takamatsu
authors.forename: M
authors.initials: M
authors.lastname: Hiramoto
authors.forename: B
authors.initials: B
authors.lastname: Shoji
authors.forename: M
authors.initials: M
authors.lastname: Takamatsu
mesh_terms: D043203:1-Alkyl-2-acetylglycerophosphocholine Esterase; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001921:Brain / Q000098:blood supply; D004907:Erythrocyte Deformability / Q000502:physiology; D004912:Erythrocytes / Q000201:enzymology*; D005260:Female; D006801:Humans; D002542:Intracranial Embolism and Thrombosis / Q000201:enzymology*; D008297:Male; D008833:Microcirculation / Q000502:physiology; D008875:Middle Aged; D010741:Phospholipases A / Q000097:blood*; D012016:Reference Values
chemical_list: D010741:Phospholipases A; D043203:1-Alkyl-2-acetylglycerophosphocholine Esterase