title: Functional analysis of pAL5000 plasmid in Mycobacterium fortuitum.
abstract: Four of the five open reading frames (ORFs) present in Myobacterium fortuitum pAL5000 plasmid (ORF1, ORF3, ORF4, and ORF5) are dispensable for replication in M. fortuitum. However, two additional ORFs (ORF1 and ORF5) were necessary for replication in a Myobacterium smegmatis heterologous host containing an efficient plasmid transformation mutation.
authors.affiliation: Centro Nacional de Investigaciones Cientificas, La Habana, Cuba.
authors.forename: C A
authors.initials: CA
authors.lastname: Villar
authors.forename: J
authors.initials: J
authors.lastname: Benitez
mesh_terms: D004261:DNA Replication; D004269:DNA, Bacterial / Q000235:genetics; D009170:Nontuberculous Mycobacteria / Q000235:genetics*; D016366:Open Reading Frames; D010957:Plasmids; D013045:Species Specificity; D014169:Transformation, Bacterial
chemical_list: D004269:DNA, Bacterial