A rapid and sensitive heterogeneous immunoelectrochemical assay using disposable electrodes.
In this novel enzyme-tagged immuoelectrochemical assay, disposable carbon felt discs serve both as electrodes and as the heterogeneous solid phase. Antibodies are immobilized on the carbon felt via a diaminoalkane-biotin-avidin-biotin bridge. Alkaline phosphatase is used as a label. Bound antibodies are monitored by following the electro-oxidation of aminophenol, produced enzymatically from p-amino-phenyl phosphate by the immobilized alkaline phosphatase at the electrode surface. A model system designed for determination of mouse IgG concentration yielded a calibration curve ranging from 10 pg/ml to 100 micrograms/ml. This assay can be performed rapidly and a single determination completed within 20 minutes. The system is useful also for rapid quantitation of a small number (approximately 80 organisms per ml) of bacteria.
Hadas
E
E
Department of Biotechnology, Faculty of Life Sciences, Tel-Aviv University, Israel.
Soussan
L
L
Rosen-Margalit
I
I
Farkash
A
A
Rishpon
J
J
D000818:Animals; D002244:Carbon; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods* / Q000706:statistics & numerical data; D004566:Electrodes; D005069:Evaluation Studies as Topic; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods* / Q000706:statistics & numerical data; D007124:Immunoenzyme Techniques / Q000295:instrumentation / Q000706:statistics & numerical data; D007074:Immunoglobulin G / Q000032:analysis; D051379:Mice; D012680:Sensitivity and Specificity; D013211:Staphylococcus aureus / Q000302:isolation & purification; D013696:Temperature
D007074:Immunoglobulin G; D002244:Carbon