[Use of Hoechst fluorescent probe 33258 for quantitative analysis of intracellular binding of anthracycline antibiotics to DNA].
The paper deals with development of a procedure for quantitative determination of the share of anthracycline antibiotics bound in cells directly to DNA. A DNA-specific Hoechst fluorescence dye 33258 was used for the purpose. The level of its quenching on DNA correlated with the quantity of the antibiotic bound to it. It was shown that the quenching of the Hoechst fluorescence dye bound to DNA was not due to the dye competition with the antibiotic for the site of bounding on DNA, as was suggested earlier. It was likely to be defined by reabsorption of the radiation by antibiotic molecules.
Egudina
S V
SV
Baranov
E P
EP
Bogush
T A
TA
Kris'ko
Sh I
ShI
D000818:Animals; D001665:Binding Sites / Q000187:drug effects / Q000502:physiology; D004247:DNA / Q000187:drug effects* / Q000378:metabolism; D004317:Doxorubicin / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000494:pharmacology*; D066298:In Vitro Techniques; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D008957:Models, Genetic; D013050:Spectrometry, Fluorescence / Q000295:instrumentation / Q000379:methods; D013950:Thymus Gland / Q000166:cytology*
D005456:Fluorescent Dyes; D004317:Doxorubicin; D004247:DNA
Ispol'zovanie fliuorestsentnogo zenda Khekhsta 33258 dlia koli- chestvennogo opredeleniia sviazyvaniia antratsiklinovykh antibiotikov s DNK v kletkakh.