Decreased hypothalamic and medullary GABA turnover in spontaneously hypertensive rats.
OBJECTIVE
The aim was to assess whether Gamma-aminobutyric acid (GABA) neurone activities in the central nervous system, especially in the hypothalamus and medulla oblangata, are altered in hypertension.


METHODS
Central GABA content and turnover rate were measured in spontaneously hypertensive rats (SHR) and their normotensive Wistar Kyoto controls (WKY). GABA content was determined with high performance liquid chromatography, and in vivo GABA turnover rates were estimated by GABA accumulation after injection of amino-oxyacetic acid, a selective inhibitor of GABA degrading system. Two groups of nine week old male rats (32 SHR and 32 WKY) were used.


RESULTS
GABA concentrations in cerebrospinal fluid were lower in SHR than in WKY. Since hypothalamus and medulla oblongata are the possible active sites of this system, basal GABA contents and in vivo GABA turnover rates were measured in hypothalamus and medulla oblongata. Basal GABA content in the medulla oblongata and hypothalamus was almost equal in SHR and WKY. On the other hand, GABA turnover rates were significantly lower in SHR than in WKY in both the hypothalamus and the medulla.


CONCLUSIONS
Since it is known that GABA is an inhibitory neurotransmitter in the central nervous system and that it controls autonomic and cardiovascular activities, the findings suggest that the decreased hypothalamic and medullary GABAergic activities may permit sympathetic hyperactivity to contribute to the increase in blood pressure in SHR.
Sasaki
S
S
Second Department of Medicine, Kyoto Prefectural University of Medicine, Japan.
Kuwabara
T
T
Yoshitomi
T
T
Yoneda
Y
Y
Takenaka
K
K
Takesako
T
T
Tanaka
M
M
Hirata
M
M
Tanabe
S
S
Nakata
T
T
D000625:Aminooxyacetic Acid / Q000494:pharmacology; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D006973:Hypertension / Q000378:metabolism*; D007031:Hypothalamus / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008526:Medulla Oblongata / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D005680:gamma-Aminobutyric Acid / Q000134:cerebrospinal fluid / Q000378:metabolism*
D000625:Aminooxyacetic Acid; D005680:gamma-Aminobutyric Acid