[Inhibition of phospholipid reacylation in oxidative damage of tumor cells].
In this study we examined the effect of oxidative stress on phospholipid reacylation and [1-14C]arachidonic acid uptake by murine mastocytoma P815 cells. Tert-butyl hydroperoxide inhibited arachidonic acid uptake by P815 cells and incorporation of fatty acid into main cellular phospholipids. Specific activity of control phosphatidylcholine was about 15-fold higher than that of phosphatidylcholine from tert-butyl hydroperoxide-treated cells. For phosphatidylethanolamine and phosphatidylinositol this inhibition was about 7-fold and 12-fold, respectively. Thus, oxidative stress causes the drastic changes in the process of phospholipid repair, which may significantly disorder membrane stability.
Kondakova
I V
IV
Borunov
E V
EV
Bondarenko
E I
EI
D000215:Acylation; D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism; D066298:In Vitro Techniques; D012515:Mast-Cell Sarcoma / Q000378:metabolism; D051379:Mice; D008811:Mice, Inbred DBA; D016877:Oxidants / Q000494:pharmacology*; D010084:Oxidation-Reduction; D010545:Peroxides / Q000494:pharmacology*; D010713:Phosphatidylcholines / Q000378:metabolism; D010714:Phosphatidylethanolamines / Q000378:metabolism; D010716:Phosphatidylinositols / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism*; D012513:Sarcoma, Experimental / Q000378:metabolism*; D020122:tert-Butylhydroperoxide
D005227:Fatty Acids; D016877:Oxidants; D010545:Peroxides; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D010716:Phosphatidylinositols; D010743:Phospholipids; D016718:Arachidonic Acid; D020122:tert-Butylhydroperoxide
Ingibirovanie reatsilirovaniia fosfolipidov pri okislitel'nom povrezhdenii opukholevykh kletok.