Maturational stage specific immunoglobulin heavy chain gene rearrangements, determined by D and D upstream region gene structures.
In 43 cases of various B-cell lineage tumors, precise gene structures of rearranged immunoglobulin heavy chain (IgH) were studied. By Southern-blot analysis of D upstream (5'D) gene of IgH, biallelic rearrangement structures, D-J or V-D-J, were determined and consequently maturational stage specific IgH rearrangement patterns were investigated. B-precursor ALL cases (especially stage IV of Nadler's criteria) have V-D-J rearranged IgH genes on both alleles. In contrast, most of the mature B-cell malignancies, excluding multiple myeloma, have IgH genotype of D-J/V-D-J. In addition, in case of D-J/V-D-J, the D gene used in D-J joining has been speculated by Southern-blot of D genes. So, these approaches for inquiring precise structures of rearranged IgH genes are supposed to provide new information of lymphocyte differentiation and leukemogenesis.
Miwa
H
H
Second Department of Internal Medicine, Mie University School of Medicine, Tsu, Japan.
Kita
K
K
Nosaka
T
T
Ikeda
T
T
Kawakami
K
K
Anazawa
H
H
Hoshino
K
K
Ohno
T
T
Honjo
T
T
Hatanaka
M
M
D000940:Antigenic Variation / Q000235:genetics; D015152:Blotting, Northern; D015139:Blotting, Southern; D002874:Chromosome Mapping; D004273:DNA, Neoplasm / Q000032:analysis*; D015326:Gene Rearrangement, B-Lymphocyte, Heavy Chain / Q000235:genetics*; D006801:Humans; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000235:genetics*; D008228:Lymphoma, Non-Hodgkin / Q000235:genetics*; D009101:Multiple Myeloma / Q000235:genetics*
D004273:DNA, Neoplasm