Determination of thevetin B in serum by fluorescence polarization immunoassay.
Thevetin B, a cardiac glycoside of Thevetia neriifolia Juss. seeds, was determined in serum by fluorescence polarization immunoassay. Anti-digitoxin antibody was used, thevetin B genin being structurally identical to digitoxigenin. Cross-reactivity of 94% was found by this method, for concentrations from 5 to 80 ng ml-1.
Uber-Bucek
E
E
Laboratoire de Pharmacognosie, Faculté des Sciences, Chatenay Malabry, France.
Hamon
M
M
Pham Huy
C
C
Dadoun
H
H
D002298:Cardenolides / Q000097:blood*; D002301:Cardiac Glycosides / Q000097:blood*; D003429:Cross Reactions; D004074:Digitoxin / Q000097:blood; D016231:Fluorescence Polarization Immunoassay / Q000379:methods; D006801:Humans
D002298:Cardenolides; D002301:Cardiac Glycosides; C077740:thevetin B; D004074:Digitoxin