Immunospecificity and affinity studies on anti-luteinizing hormone releasing hormone antibodies developed by azo-LHRH-TT and azo-LHRH-BSA conjugates.
Monoclonal and conventional antibodies against the 'self' decapeptide LHRH produced by azo-LHRH-TT and azo-LHRH-BSA conjugates were studied for immunoreactivity with native LHRH, azo-LHRH, LHRH (OH) and its fragments of sequence 1-10, 7-10, 4-10 and 4-6. The CoAb reacted 8-20% to azo-LHRH, 2-6% to LHRH-Lys, 1-3% LHRH-LYS-MDP and 0.5-1.68% to LHRH-Ala-Ala-Tuftsin but failed to react to LHRH free acid and its fragments. On the other hand MoAb reacted 4-6% to azo-LHRH and 10-18% to LHRH-Lys, 3-5% to LHRH-Ala-Ala-Tuftsin. These results indicate that the azotization drastically inhibited immunoreactivity but that the azo-LHRH-carrier conjugate preserved the immunogenic epitope responsible for the induction of an effective anti-LHRH antibody response.
Singh
V
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Institute of Self-Organising Systems and Biophysics, North-Eastern Hill University, Meghalaya, India.
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000915:Antibody Affinity; D000918:Antibody Specificity; D003429:Cross Reactions; D007987:Gonadotropin-Releasing Hormone / Q000031:analogs & derivatives* / Q000737:chemistry / Q000276:immunology*; D008969:Molecular Sequence Data; D011863:Radioimmunoassay; D012710:Serum Albumin, Bovine / Q000276:immunology*; D013745:Tetanus Toxoid / Q000276:immunology*
D000906:Antibodies; D000911:Antibodies, Monoclonal; D013745:Tetanus Toxoid; C077024:azo-LHRH-bovine serum albumin conjugate; C077023:azo-LHRH-tetanus toxoid conjugate; D012710:Serum Albumin, Bovine; D007987:Gonadotropin-Releasing Hormone