Osmostress-induced changes in yeast gene expression.
When Saccharomyces cerevisiae cells are exposed to high concentration of NaCl, they show reduced viability, methionine uptake and protein biosynthesis. Cells can acquire tolerance against a severe salt shock (up to 1.4 M NaCl) by a previous treatment with 0.7 M NaCl, but not by a previous heat shock. Two-dimensional analysis of [3H]-leucine-labelled proteins from salt-shocked cells (0.7 M NaCl) revealed the elevated rate of synthesis of nine proteins, among which were the heat-shock proteins hsp12 and hsp26. Northern analysis using gene-specific probes confirmed the identity of the latter proteins and, in addition, demonstrated the induction of glycerol-3-phosphate dehydrogenase gene expression. The synthesis of the same set of proteins is induced or enhanced upon exposure of cells to 0.8 M sucrose, although not as dramatically as in an iso-osmolar NaCl concentration (0.7 M).
Varela
J C
JC
Department of Biochemistry and Molecular Biology, Vrije Universiteit, Amsterdam, The Netherlands.
van Beekvelt
C
C
Planta
R J
RJ
Mager
W H
WH
D000222:Adaptation, Physiological / Q000235:genetics; D001483:Base Sequence; D015180:Electrophoresis, Gel, Two-Dimensional; D005656:Fungal Proteins / Q000032:analysis; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects; D008715:Methionine / Q000378:metabolism; D008969:Molecular Sequence Data; D009997:Osmotic Pressure / Q000187:drug effects; D012331:RNA, Fungal / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics* / Q000502:physiology*; D012965:Sodium Chloride / Q000494:pharmacology; D013395:Sucrose / Q000494:pharmacology; D013462:Sulfur Radioisotopes
D005656:Fungal Proteins; D012331:RNA, Fungal; D012333:RNA, Messenger; D013462:Sulfur Radioisotopes; D012965:Sodium Chloride; D013395:Sucrose; D008715:Methionine