[Genetic evidence of a species complex in Anopheles pseudopunctipennis pseudopunctipennis].
In light of inconsistencies in the pattern of malaria transmission within geographical areas inhabited by Anopheles pseudopunctipennis pseudopunctipennis, a study was carried out to investigate the possibility that this vector constitutes a species complex. Hybrid crossing studies, electrophoretic analysis of enzyme loci, and DNA restriction analysis were conducted on mosquitoes captured at nine sites in Mexico, Bolivia, and Peru. The sterility of generations resulting from cross-mating of Mexican female mosquitoes and South American male mosquitoes; the results of electrophoretic analysis, which showed differences at two loci; and a genetic distance value of 0.13 confirmed the existence of a species complex, probably produced by allopatric speciation. It is concluded that the geographic distribution of this newly discovered species complex should be defined, in view of its potential effect on malaria control.
Estrada-Franco
J G
JG
Department of Entomology, University of Maryland, College Park 20742.
Ma
M C
MC
Lanzaro
G C
GC
Gwadz
R
R
Galván-Sánchez
C
C
Céspedes
J L
JL
Vargas-Sagarnaga
R
R
Rodríguez
R
R
D000818:Animals; D000852:Anopheles / Q000145:classification* / Q000235:genetics; D001838:Bolivia; D004247:DNA / Q000032:analysis; D005260:Female; D005787:Gene Frequency; D006801:Humans; D006824:Hybridization, Genetic; D007303:Insect Vectors / Q000235:genetics*; D008288:Malaria / Q000635:transmission; D008297:Male; D008800:Mexico; D010568:Peru; D013045:Species Specificity
D004247:DNA
Evidencia genética de un complejo de especie en Anopheles pseudopunctipennis pseudopunctipennis.