title: Partial characterization of natural and recombinant human soluble CD23.
abstract: The purification to homogeneity of an active soluble 25 kDa fragment of CD23, produced in insect cells using the baculovirus expression system, is described. Peptide mapping and analysis by Edman degradation and mass spectrometry permitted partial characterization of the protein. A total of 165 out of 172 residues, including N-terminal and C-terminal regions, were mapped. The positions of the two disulphide bonds in the IgE-binding region were also determined: residue 110 is joined to residue 124, and residue 42 to residue 133. Natural CD23 25 kDa fragment was also analysed and found to possess the same disulphide bond arrangement. These results extend the previously noted sequence similarity with lectins to elements of secondary structure.
authors.affiliation: Département de Biochimie Médicale, Centre Médical Universitaire, Geneva, Switzerland.
authors.forename: K
authors.initials: K
authors.lastname: Rose
authors.forename: G
authors.initials: G
authors.lastname: Turcatti
authors.forename: P
authors.initials: P
authors.lastname: Graber
authors.forename: S
authors.initials: S
authors.lastname: Pochon
authors.forename: P O
authors.initials: PO
authors.lastname: Regamey
authors.forename: K U
authors.initials: KU
authors.lastname: Jansen
authors.forename: E
authors.initials: E
authors.lastname: Magnenat
authors.forename: N
authors.initials: N
authors.lastname: Aubonney
authors.forename: J Y
authors.initials: JY
authors.lastname: Bonnefoy
mesh_terms: D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid; D004220:Disulfides / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007073:Immunoglobulin E / Q000378:metabolism; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D010449:Peptide Mapping; D017455:Receptors, IgE / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification; D014357:Trypsin
chemical_list: D004220:Disulfides; D017455:Receptors, IgE; D011994:Recombinant Proteins; D007073:Immunoglobulin E; D014357:Trypsin