[Diagnosis of Helicobacter pylori infection. Rapid urease test, microscopy of smears and culture from ventricular biopsy compared with the 14C-urea breath test].
By employing the 14C-urea breath test as the reference method we determined the specificity and sensitivity of three bioptic methods for diagnosis of Helicobacter pylori infection in 103 subjects. All biopsy specimens were obtained from the gastric antrum. For culture the specificity was 100%. Its applicability was reduced, however, by a low sensitivity (73.8%) and a delay of several days before the final result was available. Microscopy of Loeffler-stained biopsy smears yielded a specificity of 100% and a sensitivity of 92.9%, but the method was regarded time-consuming. The rapid urease test yielded a specificity of 98.4% and a sensitivity of 85.7%. Being quick, simple and inexpensive the rapid urease test is well suited for routine use in gastroscopy.
Nysaeter
G
G
Gastroenterologisk seksjon, Haukeland sykehus, Bergen.
Berstad
K
K
Weberg
R
R
Berstad
A
A
Hardardottir
H
H
D001431:Bacteriological Techniques; D001944:Breath Tests / Q000379:methods*; D005756:Gastritis / Q000175:diagnosis* / Q000382:microbiology / Q000473:pathology; D005773:Gastroscopy; D016481:Helicobacter Infections / Q000175:diagnosis* / Q000382:microbiology / Q000473:pathology; D016480:Helicobacter pylori / Q000302:isolation & purification*; D006801:Humans; D011706:Pyloric Antrum / Q000382:microbiology / Q000473:pathology; D014508:Urea / Q000032:analysis; D014510:Urease
D014508:Urea; D014510:Urease
Diagnostikk av Helicobacter pylori-infeksjon. Ureasehurtigtest, mikroskopi av utstryk og dyrking frå ventrikkelbiopsi samanlikna med 14C-urea-pusteprøven.