title: Determination of vinorelbine (Navelbine) in tumour cells by high-performance liquid chromatography.
abstract: A high-performance liquid chromatographic method has been developed for the determination within tumour cells of a new vinca alkaloid, vinorelbine. Extractions of vinorelbine from cells were carried out using absolute ethanol. The extracts were injected into a reversed-phase system consisting of two Novapak C18 columns connected in series. The mobile phase was acetonitrile-phosphate buffer, pH 2.7 (60:40, v/v). Using a fluorescence detection, the limit of determination was 8 pmol injected. This method would be suitable for studying the cellular pharmacokinetics of vinorelbine in patients.
authors.affiliation: Laboratoire de Spectroscopie Biomoléculaire, U.F.R. de Pharmacie, Reims, France.
authors.forename: V
authors.initials: V
authors.lastname: Debal
authors.forename: H
authors.initials: H
authors.lastname: Morjani
authors.forename: J M
authors.initials: JM
authors.lastname: Millot
authors.forename: J F
authors.initials: JF
authors.lastname: Angiboust
authors.forename: B
authors.initials: B
authors.lastname: Gourdier
authors.forename: M
authors.initials: M
authors.lastname: Manfait
mesh_terms: D000970:Antineoplastic Agents / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007951:Leukemia, Myeloid; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence; D014407:Tumor Cells, Cultured / Q000737:chemistry*; D014747:Vinblastine / Q000031:analogs & derivatives* / Q000032:analysis; D000077235:Vinorelbine
chemical_list: D000970:Antineoplastic Agents; D014747:Vinblastine; D000077235:Vinorelbine