Peroxidase-antiperoxidase labelling of Leishmania amastigotes in formalin fixed and paraffin embedded tissue sections.
A modified PAP-Method (peroxidase-antiperoxidase) is established for laboratory use to demonstrate Leishmania spp. amastigotes in paraffin sections of experimentally and naturally infected dogs. The results demonstrate that the PAP technique is a valuable tool to detect Leishmania amastigotes in tissue sections with low parasite density.
Wunderlin
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Institute of Veterinary Pathology, University of Zurich, Switzerland.
Pospischil
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A
D000818:Animals; D004283:Dog Diseases / Q000469:parasitology*; D004285:Dogs; D005557:Formaldehyde; D007124:Immunoenzyme Techniques; D007891:Leishmania / Q000302:isolation & purification*; D007896:Leishmaniasis / Q000469:parasitology / Q000662:veterinary*; D016612:Paraffin Embedding; D012189:Retrospective Studies
D005557:Formaldehyde