High-performance liquid chromatography of oligosaccharide alditols and glycopeptides on a graphitized carbon column.
The chromatographic behaviour of oligosaccharide alditols and glycopeptides containing neutral and acetamido sugars and sialic acid has been investigated on a HyperCarb porous graphitised carbon column. The alditols were substantially retained and could be eluted in 0-25% acetonitrile-0.05% trifluoroacetic acid in 0.05% aqueous trifluoroacetic acid between 3-30 min for mono- to hexasaccharides. Elution patterns were based on both size, charge and linkage such that isomeric compounds could be separated from each other.
Davies
M
M
Glycoconjugates Section, Clinical Research Centre, Harrow, Middlesex, UK.
Smith
K D
KD
Harbin
A M
AM
Hounsell
E F
EF
D000595:Amino Acid Sequence; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006020:Glycopeptides / Q000737:chemistry / Q000302:isolation & purification*; D006108:Graphite; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry / Q000302:isolation & purification*; D013402:Sugar Alcohols / Q000737:chemistry / Q000302:isolation & purification*
D006020:Glycopeptides; D009844:Oligosaccharides; D013402:Sugar Alcohols; D006108:Graphite