Effects of interleukin 2 on engraftment following autologous bone marrow transplantation (ABMT) in dogs.
Beagle dogs were treated with recombinant human interleukin 2 (IL-2) 6 x 10(6) International Units (IU)/day for 7 consecutive days following conditioning with sublethal (200 cGy) or lethal (400 cGy) doses of high-dose rate whole body irradiation (WBI) and reconstitution with 2 x 10(8)/kg autologous bone marrow cells, in order to assess the effect of IL-2 on engraftment. Engraftment of dogs conditioned by lethal doses of WBI was not impaired following treatment with IL-2 6 x 10(6) IU/day. At an RIL-2 dose of 6 x 10(6) and 9 x 10(4) IU/day, enhanced engraftment of autologous bone marrow cells was observed in dogs irradiated with a sublethal WBI dose in comparison with controls not treated by IL-2(p < 0.05). We conclude that therapeutic doses of IL-2 may be safely utilized during hematopoietic reconstitution. Under certain conditions IL-2 may even enhance hematopoietic reconstitution following ABMT.
Samuel
S
S
Department of Bone Marrow Transplantation, Hadassah University Hospital, Jerusalem, Israel.
Nagler
A
A
Or
R
R
Slavin
S
S
D000818:Animals; D016026:Bone Marrow Transplantation; D004285:Dogs; D006085:Graft Survival / Q000187:drug effects; D006454:Hemoglobins / Q000032:analysis; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology*; D007958:Leukocyte Count / Q000187:drug effects; D008297:Male; D010976:Platelet Count / Q000187:drug effects; D011994:Recombinant Proteins / Q000494:pharmacology; D014916:Whole-Body Irradiation
D006454:Hemoglobins; D007376:Interleukin-2; D011994:Recombinant Proteins