A perfused-cuvette method for fluorimetric studies of non-adherent cells.
We have developed a system for immobilizing non-adherent cells for use in macroscopic fluorescence measurements in a perfused cuvette. Using normal human T lymphocytes loaded with the fluorescent Ca(2+)-indicator, fluo-3, we have validated the properties of these cells immobilized on clear, non-fluorescent fluorohalocarbon film (Aclar) using the non-charged cell adhesive, Cell-Tak.
Schlichter
L C
LC
Playfair Neuroscience Unit, Toronto Hospital, Ontario, Canada.
Pennefather
P S
PS
van Staden
C J
CJ
Valentine
E A
EA
D002469:Cell Separation / Q000379:methods*; D006801:Humans; D010477:Perfusion / Q000379:methods*; D013050:Spectrometry, Fluorescence / Q000379:methods; D013601:T-Lymphocytes