title: AKT2, a putative oncogene encoding a member of a subfamily of protein-serine/threonine kinases, is amplified in human ovarian carcinomas.
abstract: We isolated cDNA clones containing the entire coding region of the putative oncogene AKT2. Sequence analysis and in vitro translation demonstrated that AKT2 encodes a 56-kDa protein with homology to serine/threonine kinases; moreover, this protein contains a Src homology 2-like domain. AKT2 was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. AKT2 was mapped to chromosome region 19q13.1-q13.2 by fluorescence in situ hybridization. In the two ovarian carcinoma cell lines exhibiting amplification of AKT2, the amplified sequences were localized within homogeneously staining regions. We conclude that AKT2 belongs to a distinct subfamily of protein-serine/threonine kinases containing Src homology 2-like domains and that alterations of AKT2 may contribute to the pathogenesis of ovarian carcinomas.
authors.affiliation: Department of Medical Oncology, Fox Chase Cancer Center, Philadelphia, PA 19111.
authors.forename: J Q
authors.initials: JQ
authors.lastname: Cheng
authors.forename: A K
authors.initials: AK
authors.lastname: Godwin
authors.forename: A
authors.initials: A
authors.lastname: Bellacosa
authors.forename: T
authors.initials: T
authors.lastname: Taguchi
authors.forename: T F
authors.initials: TF
authors.lastname: Franke
authors.forename: T C
authors.initials: TC
authors.lastname: Hamilton
authors.forename: P N
authors.initials: PN
authors.lastname: Tsichlis
authors.forename: J R
authors.initials: JR
authors.lastname: Testa
mesh_terms: D000595:Amino Acid Sequence; D002877:Chromosomes, Human / Q000648:ultrastructure; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D005456:Fluorescent Dyes; D005784:Gene Amplification; D015723:Gene Library; D006801:Humans; D008214:Lymphocytes / Q000166:cytology; D008969:Molecular Sequence Data; D005810:Multigene Family; D015513:Oncogene Proteins / Q000235:genetics*; D009857:Oncogenes; D010051:Ovarian Neoplasms / Q000201:enzymology / Q000235:genetics*; D014176:Protein Biosynthesis; D017346:Protein Serine-Threonine Kinases / Q000235:genetics*; D011518:Proto-Oncogene Proteins; D051057:Proto-Oncogene Proteins c-akt; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic
chemical_list: D005456:Fluorescent Dyes; D015513:Oncogene Proteins; D011518:Proto-Oncogene Proteins; D004247:DNA; C494921:AKT2 protein, human; D017346:Protein Serine-Threonine Kinases; D051057:Proto-Oncogene Proteins c-akt