title: Detection of 2-amino-1-methyl-6-(4-hydroxyphenyl)imidazo[4,5-b]pyridine in broiled beef.
abstract: 2-Amino-1-methyl-6-(4-hydroxyphenyl)imidazo[4,5-b]pyridine (4'-OH-PhIP) showed mutagenicity in Salmonella typhimurium TA98 in the presence of S9 mix, inducing 180 revertants per 100 micrograms. Since creatinine, tyrosine and glucose, which are present in meat, are expected to be involved in the formation of 4'-OH-PhIP, its presence in broiled beef was examined. 4'-OH-PhIP was detected in broiled beef by high-performance liquid chromatography and UV-spectrometry after extraction with 0.1 N HCl and purification by blue cotton treatment and ion exchange column chromatography. Its level was estimated to be 21.0 ng per g of broiled beef, which is comparable to that of 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP).
authors.affiliation: Carcinogenesis Division, National Cancer Center Research Institute, Tokyo.
authors.forename: R
authors.initials: R
authors.lastname: Kurosaka
authors.forename: K
authors.initials: K
authors.lastname: Wakabayashi
authors.forename: H
authors.initials: H
authors.lastname: Ushiyama
authors.forename: H
authors.initials: H
authors.lastname: Nukaya
authors.forename: N
authors.initials: N
authors.lastname: Arakawa
authors.forename: T
authors.initials: T
authors.lastname: Sugimura
authors.forename: M
authors.initials: M
authors.lastname: Nagao
mesh_terms: D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D007093:Imidazoles / Q000302:isolation & purification* / Q000633:toxicity; D008460:Meat / Q000032:analysis*; D009153:Mutagens / Q000633:toxicity*; D011725:Pyridines / Q000302:isolation & purification* / Q000633:toxicity; D012486:Salmonella typhimurium / Q000187:drug effects; D013056:Spectrophotometry, Ultraviolet
chemical_list: D007093:Imidazoles; D009153:Mutagens; D011725:Pyridines; C077584:2-amino-1-methyl-6-(4-hydroxyphenyl)imidazo(4,5-b)pyridine