A subgroup of class alpha glutathione S-transferases. Cloning of cDNA for mouse lung glutathione S-transferase GST 5.7.
A full-length cDNA clone encoding the previously purified mouse glutathione S-transferase GST 5.7 [(1991), Biochem. J. 278, 793-799] has been isolated from a mouse lung cDNA library in lambda gt11. Sequencing of the clone revealed the presence of microheterogeneity in GST 5.7. Comparison of the deduced protein sequence with other glutathione S-transferases, together with previous information available on GST 5.7, indicates that the enzyme belongs to a novel subgroup within the alpha class of glutathione S-transferases. Members of the subgroup, which also include the rat GST 8-8 and perhaps chicken GST CL3, show high sequence homology with each other, but only moderate similarity to other alpha class enzymes. They share a substrate specificity profile that resembles pi-class enzymes, and are active in the conjugation of lipid peroxidation products.
Zimniak
P
P
Department of Medicine, University of Arkansas for Medical Sciences, Little Rock 72205.
Eckles
M A
MA
Saxena
M
M
Awasthi
Y C
YC
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D005982:Glutathione Transferase / Q000737:chemistry / Q000235:genetics*; D008168:Lung / Q000201:enzymology*; D051379:Mice; D008969:Molecular Sequence Data; D013379:Substrate Specificity
D005982:Glutathione Transferase