Simultaneous determination of tenidap and its stable isotope analog in serum by high-performance liquid chromatography/atmospheric pressure chemical ionization tandem mass spectrometry.
A specific high-performance liquid chromatographic/atmospheric pressure chemical ionization tandem mass spectrometric assay for the quantitative determination of tenidap and its D3 analog in human serum is described. The assay exhibited a linear dynamic range of 0.1-25 micrograms ml-1. Its precision for the two analytes over the range was 17% or better. The assay validity and its utility to investigate changes in tenidap bioequivalence and pharmacokinetics are presented.
Avery
M J
MJ
Central Research Division, Pfizer Inc., Groton, Connecticut 06340.
Mitchell
D Y
DY
Falkner
F C
FC
Fouda
H G
HG
D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000097:blood*; D006801:Humans; D007211:Indoles / Q000097:blood*; D013058:Mass Spectrometry / Q000379:methods*; D000078183:Oxindoles
D000894:Anti-Inflammatory Agents, Non-Steroidal; D007211:Indoles; D000078183:Oxindoles; C062866:tenidap