title: Measurement of dothiepin and its major metabolites in plasma by high-performance liquid chromatography.
abstract: This paper describes a reversed-phase high-performance liquid chromatographic method which will simultaneously measure dothiepin and its three major metabolites (northiaden, northiaden-S-oxide and dothiepin-S-oxide) in plasma using trimipramine as internal standard. Sample preparation involved a basic extraction using diethyl ether followed by an acid back-extraction. The method we report is linear over the range 50-1000 ng/ml (r = 0.999), for all analytes. Total imprecision is less than 11% (coefficient of variation) and accuracy is greater than 94% (n = 20). Recovery of analytes varied considerably from 51.7% for northiaden-S-oxide to 90.2% for dothiepin-S-oxide.
authors.affiliation: Department of Clinical Pharmacology, Princess Alexandra Hospital, Woolloongabba, Queensland, Australia.
authors.forename: P J
authors.initials: PJ
authors.lastname: Taylor
authors.forename: B G
authors.initials: BG
authors.lastname: Charles
authors.forename: R
authors.initials: R
authors.lastname: Norris
authors.forename: P
authors.initials: P
authors.lastname: Salm
authors.forename: P J
authors.initials: PJ
authors.lastname: Ravenscroft
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004308:Dothiepin / Q000097:blood*; D006801:Humans; D012015:Reference Standards; D015203:Reproducibility of Results; D014299:Trimipramine / Q000097:blood
chemical_list: D014299:Trimipramine; D004308:Dothiepin