Localization of components of the renin-angiotensin system within the kidney and sustained release of angiotensins from isolated and perfused kidney.
We found colocalization of renin, angiotensin (Ang) I and Ang II in juxtaglomerular (JG) cells of the kidney. Coexistence of Ang II in renin granules was demonstrated by electron microscopic immunogold labeling of these components. Coexistence of both Ang I and Ang II in the high density renin storage granules were also demonstrated by gradient centrifugation of renal homogenate. These findings supported the synthesis of Ang I and Ang II in juxtaglomerular cells. Isolated and cultured JG cells showed the synthesis of Ang I, Ang II and renin. Ang I and Ang II were secreted from isolated and perfused rat kidneys at steady rates over 2 hr. Their secretion rates were proportional to that of renin. The rate of Ang II secretion from the kidney was higher than that from the vascular bed. Ang II was also found in renal lymph. These findings indicate that a large amount of Ang II is generated in JG cells by the intracellular action of renin and may play a significant role in the regulation of renal function.
Inagami
T
T
Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, Tennessee 37232.
Mizuno
K
K
Kawamura
M
M
Okamura
T
T
Clemens
D L
DL
Higashimori
K
K
D000809:Angiotensins / Q000378:metabolism*; D000818:Animals; D006801:Humans; D066298:In Vitro Techniques; D007668:Kidney / Q000378:metabolism*; D010477:Perfusion; D012084:Renin-Angiotensin System / Q000502:physiology*
D000809:Angiotensins