An animal model for extracorporeal photochemotherapy based on contact hypersensitivity.
Recently, photopheresis was introduced as a specific immune suppressor in several T cell mediated disorders. In order to study photopheresis, animal models are indispensable. This report describes an easy to handle model for this purpose. It concerns the Wistar-derived rat with contact hypersensitivity (CHS), also a T cell mediated disorder that has already been studied extensively in several other fields of research. After subsequent exposure to 8-methoxypsoralen (8-MOP) and ultraviolet A radiation (UVA), white blood cells from CHS rats were intravenously injected into other syngeneic rats suffering from the same disorder. This treatment appears to be very efficacious in suppressing the immunological response against the applied contact allergen, 2,4-dinitrofluorobenzene (DNFB). Cells subsequently exposed to UVA and 8-MOP did not have any effect.
van Iperen
H P
HP
Department of Medicinal Photochemistry, State University of Leiden, Netherlands.
Beijersbergen van Henegouwen
G M
GM
D000096:Acetone; D000485:Allergens; D000818:Animals; D003877:Dermatitis, Contact / Q000628:therapy*; D004139:Dinitrofluorobenzene; D004195:Disease Models, Animal; D007165:Immunosuppression Therapy; D008297:Male; D008730:Methoxsalen / Q000494:pharmacology*; D010778:Photochemotherapy / Q000379:methods; D051381:Rats; D017208:Rats, Wistar; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology*; D014466:Ultraviolet Rays
D000485:Allergens; D000096:Acetone; D004139:Dinitrofluorobenzene; D008730:Methoxsalen