Fatty acids are precursors of alkylamines in Deinococcus radiodurans.
Deinococcus radiodurans contains novel phospholipids of which the structures of three have been previously described. These three lipids contain both fatty acids and alkylamines. Both the fatty acid and alkylamine constituents were found to be composed of a mixture of species, of which C15, C16, and C17 saturated and monounsaturated alkyl chains predominated. Alkylamines contained a relatively higher proportion of saturated species. Progression of bacterial growth through the mid-log to stationary phases was accompanied by an increase in the proportions of C15 and C17 alkyl chains in both fatty acid and alkylamine constituents. Radiolabeled palmitic acid was found to be rapidly incorporated into both fatty acid and alkylamine components of phosphatidylglyceroylalkylamine, which is the precursor of the more-complex phosphoglycolipids found in major amounts in D. radiodurans. After culturing D. radiodurans in the presence of a mixture of palmitic acids labeled with 14C and 3H in the 1 and 9,10 positions, respectively, the same 14C/3H ratio was recovered in both fatty acid and alkylamine constituents, strongly suggesting that alkylamines are derived from intact fatty acids rather than by a de novo pathway. The results identify a novel product of fatty acid metabolism which has not to date been observed in any other organism.
Anderson
R
R
Department of Microbiology and Infectious Diseases, University of Calgary, Alberta, Canada.
Huang
Y
Y
D000478:Alkylation; D001345:Autoradiography; D002250:Carbon Radioisotopes; D006017:Glycolipids / Q000096:biosynthesis* / Q000737:chemistry; D006095:Gram-Positive Cocci / Q000378:metabolism*; D019308:Palmitic Acid; D010169:Palmitic Acids / Q000378:metabolism*; D010743:Phospholipids / Q000096:biosynthesis* / Q000737:chemistry; D014316:Tritium
D002250:Carbon Radioisotopes; D006017:Glycolipids; D010169:Palmitic Acids; D010743:Phospholipids; D014316:Tritium; D019308:Palmitic Acid