Evidence for a single catalytic site of honeybee alpha-glucosidase I by chemical modification with diethylpyrocarbonate.
Honeybee alpha-glucosidase I was inactivated with diethylpyrocarbonate (DEPC). The inactivation followed pseudo-first-order kinetics. The rate of the loss of activity was decreased by the addition of a substrate, maltose. Since there was no spectral change in the tyrosine absorption region, it was recognized that DEPC did not react with this residue. The alpha-glucosidase had one free sulfhydryl group, which was not involved in the catalytic reaction, and was not modified by DEPC. On the other hand, the specific reaction of DEPC with a histidyl residue was spectrophotometrically confirmed by an increase in absorption near 240 nm, and the activity of the inactivated enzyme was restored by hydroxylamine. The modification rate of one histidyl residue by DEPC was almost equal to the rate of the activity loss. These results indicate that there is one histidyl residue at or near the catalytic site, and that honeybee alpha-glucosidase I has a single active site.
Kimura
A
A
Department of Agricultural Chemistry, Faculty of Agriculture, Hokkaido University.
Matsui
H
H
Honma
M
M
Chiba
S
S
D000818:Animals; D001516:Bees / Q000201:enzymology*; D002384:Catalysis / Q000187:drug effects; D004047:Diethyl Pyrocarbonate / Q000737:chemistry / Q000494:pharmacology*; D004789:Enzyme Activation / Q000187:drug effects; D006639:Histidine / Q000737:chemistry; D007527:Isoenzymes / Q000737:chemistry* / Q000187:drug effects; D007700:Kinetics; D000520:alpha-Glucosidases / Q000737:chemistry* / Q000187:drug effects
D007527:Isoenzymes; D006639:Histidine; D000520:alpha-Glucosidases; D004047:Diethyl Pyrocarbonate