Pathogenicity and immunogenicity of recombinant human retinal S-antigen fusion protein.
A full-length cDNA clone to human S-antigen (HS-ag) was isolated from lambda gt 10 human retinal library and expressed as a fusion protein with glutathione S-transferase (GST) in E. Coli. Uveitogenicity and immunogenicity of recombinant GST-HS-ag fusion protein and native HS-ag were compared in EAU-susceptible Lewis rats. Recombinant HS-ag was found less uveitogenic than native HS-ag. Animals inoculated with recombinant HS-ag developed EAU on day 17, three days later than those inoculated with native HS-ag, the incidence of the disease was reduced from 80% to 58% and the score of clinical severity reduced from 2.2 to 1.3 points respectively. In contrast, rGST-HS-ag was more immunogenic than native HS-ag as it elicited four times higher levels of antibodies which reacted specifically with both antigens.
Kasp
E
E
Department of Immunology, Rayne Institute, St Thomas' Hospital, UMDS, London, UK.
Roberts
A J
AJ
Stanford
M R
MR
Whiston
R
R
Dumonde
D C
DC
Banga
J P
JP
D000818:Animals; D000941:Antigens / Q000235:genetics / Q000276:immunology*; D019393:Arrestin; D001324:Autoantigens / Q000276:immunology*; D004195:Disease Models, Animal; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000235:genetics; D005136:Eye Proteins / Q000235:genetics / Q000276:immunology*; D015870:Gene Expression; D005982:Glutathione Transferase / Q000276:immunology*; D006801:Humans; D015994:Incidence; D008297:Male; D051381:Rats; D011917:Rats, Inbred Lew; D011993:Recombinant Fusion Proteins / Q000276:immunology*; D014605:Uveitis / Q000276:immunology* / Q000473:pathology*
D000941:Antigens; D019393:Arrestin; D001324:Autoantigens; D005136:Eye Proteins; D011993:Recombinant Fusion Proteins; D005982:Glutathione Transferase