title: FinOP repression of the F plasmid involves extension of the half-life of FinP antisense RNA by FinO.
abstract: The transfer operon of the F plasmid is positively regulated by the traJ gene product, expression of which, in turn, is regulated by both an antisense RNA, FinP, and the FinO protein (the FinOP system). A finP- F plasmid, pSFL20, was constructed by site-directed mutagenesis and was found to produce wild-type levels of pili encoded by the transfer operon. Transcription of the traJ gene was decreased by a factor of 3-5 fold in the presence of FinOP with no accumulation of a stable RNA duplex between the FinP RNA and the portion of the traJ mRNA which is complementary to finP. Stabilization of FinP RNA by FinO occurs in the absence of traJ transcripts, suggesting that FinO may interact directly with FinP to prevent its degradation.
authors.affiliation: Department of Biochemistry, University of Alberta, Edmonton, Canada.
authors.forename: S H
authors.initials: SH
authors.lastname: Lee
authors.forename: L S
authors.initials: LS
authors.lastname: Frost
authors.forename: W
authors.initials: W
authors.lastname: Paranchych
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D001426:Bacterial Proteins / Q000378:metabolism*; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial; D004722:Endoribonucleases / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D029968:Escherichia coli Proteins; D005144:F Factor; D015964:Gene Expression Regulation, Bacterial; D006207:Half-Life; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009876:Operon; D011401:Promoter Regions, Genetic; D016372:RNA, Antisense / Q000378:metabolism*; D016601:RNA-Binding Proteins; D012097:Repressor Proteins; D014158:Transcription, Genetic
chemical_list: D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; D016372:RNA, Antisense; D016601:RNA-Binding Proteins; D012097:Repressor Proteins; C488999:TraJ protein, E coli; C041427:TraJ protein, bacteria; C074275:FinO protein, E coli; D004722:Endoribonucleases; C022537:ribonuclease E