Pharmacology of the 5-hydroxytryptamine-induced depolarization of the ferret vagus nerve in vitro.
Grease-gap recordings revealed that 5-hydroxytryptamine (5-HT) depolarized the ferret vagus nerve (pEC50 = 4.9). This response was mimicked by 2-methyl-5-HT and 1-phenylbiguanide, but not by 5-carboxamidotryptamine. Paroxetine (1 microM) or ketamine (10 microM) did not potentiate the response. Ketanserin (1 microM) did not reduce the depolarization, but four 5-HT3 receptor antagonists did. It is concluded that 5-HT depolarizes the ferret vagus nerve via 5-HT3 receptors, but these receptors may differ pharmacologically from those in other species.
Newberry
N R
NR
Oxford University, SmithKline Beecham Centre for Applied Neuropsychobiology, Department of Clinical Pharmacology, Radcliffe Infirmary, UK.
Watkins
C J
CJ
Reynolds
D J
DJ
Leslie
R A
RA
Grahame-Smith
D G
DG
D000818:Animals; D004305:Dose-Response Relationship, Drug; D005260:Female; D005289:Ferrets; D066298:In Vitro Techniques; D008564:Membrane Potentials / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D011985:Receptors, Serotonin / Q000187:drug effects; D012701:Serotonin / Q000031:analogs & derivatives / Q000494:pharmacology*; D014403:Tubocurarine / Q000494:pharmacology; D014630:Vagus Nerve / Q000187:drug effects* / Q000502:physiology
D011985:Receptors, Serotonin; D012701:Serotonin; C045918:2-methyl-5-HT; D014403:Tubocurarine