Hepatic heparan sulphate proteoglycan and the recycling of transferrin.
Heparan sulphate proteoglycan, labelled with [35S]sulphate, was prepared from rat livers for studies of its interaction with purified rat transferrin. Affinity chromatography of the preparation on columns of immobilized differic transferrin and apotransferrin showed that the proteoglycan possessed affinity for both types of matrices at pH 7.3 and that this affinity significantly increased at pH 5.6. The glycosaminoglycan chains liberated from the proteoglycan by heparan sulphate lyase also bound to apotransferrin, albeit less strongly, whereas the deglycosylated core protein exhibited virtually no interaction with this matrix. In the presence of the proteoglycan at pH 5.6, the release of iron from the N-lobe of transferrin was accelerated. These observations suggest that heparan sulphate proteoglycan from the liver can mimick some of the known functions of bona fide transferrin receptors and, hence, interaction with the proteoglycan may provide an alternative nondegradative pathway for transferrin through hepatic cells.
Hu
W L
WL
Department of Pathology, McMaster University Health Sciences Centre, Hamilton, Ont., Canada.
Regoeczi
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D000818:Animals; D001059:Apoproteins / Q000378:metabolism; D002846:Chromatography, Affinity; D019812:Heparan Sulfate Proteoglycans; D006497:Heparan Sulfate / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007501:Iron / Q000378:metabolism; D008099:Liver / Q000378:metabolism*; D011509:Proteoglycans / Q000378:metabolism*; D051381:Rats; D011990:Receptors, Transferrin / Q000378:metabolism; D014168:Transferrin / Q000378:metabolism*
D001059:Apoproteins; D019812:Heparan Sulfate Proteoglycans; D006497:Heparan Sulfate; D007501:Iron; D011509:Proteoglycans; D011990:Receptors, Transferrin; D014168:Transferrin; C028284:apotransferrin