title: Influence of reagent composition on atypical pseudocholinesterase activity measurement: comparison of a manual and an automated method and implications for routine.
abstract: In determining activities of atypical pseudocholinesterase using a Hitachi 737 and a Cobas Bio analyser, respectively, we repeatedly found discrepancies between the enzyme activities measured by both instruments which were presumably due to differences in the reagent composition (Hitachi 737: Boehringer Mannheim Automated Analysis for BM/Hitachi Systems; Cobas Bio: Boehringer Mannheim Monotest). We consider the present results as a strong hint for manufacturers towards generally declaring all components of the chemical systems they produce and as a warning for users when interchanging apparently identically composed sets of reagents.
authors.affiliation: Dept. of Clinical Chemistry, Philipps-University, Marburg, Germany.
authors.forename: N B
authors.initials: NB
authors.lastname: Krull
authors.forename: J
authors.initials: J
authors.lastname: Kropf
authors.forename: A M
authors.initials: AM
authors.lastname: Gressner
mesh_terms: D002091:Butyrylcholinesterase / Q000378:metabolism*; D002624:Chemistry, Clinical / Q000379:methods; D011933:Reagent Kits, Diagnostic / Q000592:standards
chemical_list: D011933:Reagent Kits, Diagnostic; D002091:Butyrylcholinesterase