Expression of Japanese quail ovalbumin in Saccharomyces cerevisiae.
A cDNA sequence coding for Japanese quail ovalbumin was used for the construction of expression plasmid under the ADH1 promoter of the yeast shuttle vector pVT101-U. The resulting recombinant expression vector pJK2 was used for the transformation of Saccharomyces cerevisiae. Expression of quail ovalbumin in yeast cells was demonstrated by Western blotting followed by immunochemical detection.
Krizková
L
L
Laboratory of Genetic Engineering, Slovak Academy of Sciences, Bratislava.
Jeloková
J
J
Klaudíny
J
J
Zámocký
M
M
D000818:Animals; D003370:Coturnix; D004247:DNA / Q000235:genetics; D015870:Gene Expression / Q000235:genetics; D005822:Genetic Vectors; D010047:Ovalbumin / Q000096:biosynthesis / Q000235:genetics*; D010957:Plasmids; D011994:Recombinant Proteins / Q000096:biosynthesis; D012441:Saccharomyces cerevisiae / Q000235:genetics*
D011994:Recombinant Proteins; D010047:Ovalbumin; D004247:DNA