Detection and identification of mammalian DNA from the gut of museum specimens of ticks.
A method for detection and identification of mammalian DNA in the gut of alcohol-preserved ticks has been developed. Successful amplification and sequencing of host cytochrome b is reported from ticks preserved in 70% alcohol for more than 40 yr. The method described shows promise for determination of unknown tick hosts.
Tobolewski
J
J
Department of Zoology, Brigham Young University, Provo, UT 84602.
Kaliszewski
M J
MJ
Colwell
R K
RK
Oliver
J H
JH
D000818:Animals; D001096:Arachnid Vectors; D001483:Base Sequence; D003573:Cytochrome b Group / Q000235:genetics; D004247:DNA / Q000235:genetics / Q000302:isolation & purification*; D008322:Mammals; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D013987:Ticks
D003573:Cytochrome b Group; D004247:DNA