title: Anti-dsDNA and Sm autoantibodies in systemic lupus erythematosus.
abstract: During the present study the coincidence of anti-dsDNA and Sm antibodies was detected in 16 percent of 51 consecutive SLE patients. These antibodies were detected by the standard indirect immunofluorescence and Ouchterlony tests. All patients with anti-dsDNA and Sm antibodies showed disease activity, including renal, CNS and pulmonary disease. We excluded a cross reactivity of these antibodies by ELISA, using competitive experiments with dsDNA and Sm antigens. The results support the presence of multiple autoantibody production during SLE activity, and suggest that different mechanisms may underlie the induction and regulation of both autoantibodies.
authors.affiliation: Departamento de Investigación, Hospital 20 de Noviembre, ISSSTE, México.
authors.forename: E
authors.initials: E
authors.lastname: Martínez-Cordero
authors.forename: E
authors.initials: E
authors.lastname: Martínez-Miranda
authors.forename: M C
authors.initials: MC
authors.lastname: Negrete-García
authors.forename: A
authors.initials: A
authors.lastname: Padilla
authors.forename: D E
authors.initials: DE
authors.lastname: Aguilar León
mesh_terms: D000293:Adolescent; D000328:Adult; D000974:Antibodies, Antinuclear / Q000032:analysis*; D001323:Autoantibodies / Q000032:analysis* / Q000276:immunology; D001324:Autoantigens / Q000276:immunology*; D003429:Cross Reactions; D004247:DNA / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D017411:Ribonucleoproteins, Small Nuclear; D055517:snRNP Core Proteins
chemical_list: D000974:Antibodies, Antinuclear; D001323:Autoantibodies; D001324:Autoantigens; D017411:Ribonucleoproteins, Small Nuclear; D055517:snRNP Core Proteins; D004247:DNA