Bovine plasma beta-mannosidase activity and its potential use for beta-mannosidosis carrier detection.
Plasma beta-mannosidase activities were determined for Salers cattle from 8 herds as an evaluation of this method for detection of beta-mannosidosis heterozygotes. Several biological factors, such as age, gender, herd, and risk of being a beta-mannosidosis carrier, were considered in this study. The mean enzyme activity for obligate heterozygotes (n = 8) was 55 U/ml (range = 43-65 U/ml), which was 59% of the mean enzyme activity for cattle that were low risk for being a carrier. These data indicate that bovine beta-mannosidosis is characterized by a gene dosage effect. The analytical and biological variation of plasma beta-mannosidase activity that was observed necessitates limiting the test to adult fullblood/purebred Salers cattle within a herd. Plasma beta-mannosidase analysis provides important information for intraherd selection of Salers cattle that are heterozygous for beta-mannosidosis.
Cavanagh
K T
KT
Department of Pathology, Michigan State University, East Lansing 48824.
Jones
M Z
MZ
Abbitt
B
B
Skinner
R
R
D000375:Aging; D000704:Analysis of Variance; D000818:Animals; D002417:Cattle / Q000002:abnormalities* / Q000097:blood; D005260:Female; D006580:Genetic Carrier Screening / Q000379:methods*; D008297:Male; D008361:Mannosidases / Q000097:blood*; D015203:Reproducibility of Results; D008363:alpha-Mannosidosis / Q000235:genetics / Q000662:veterinary*; D044902:beta-Mannosidase
D008361:Mannosidases; D044902:beta-Mannosidase