In vivo measurement of atrial natriuretic peptide receptors using nuclear imaging.
We have successfully visualized atrial natriuretic peptide (ANP) receptors in vivo using nuclear imaging. 123I-Labelled ANP, injected in green vervet monkeys, was rapidly bound to ANP receptors in the kidneys and lungs. That the observed uptake was receptor mediated was demonstrated with competition studies using simultaneous injection of unlabelled ANP 99-126. It was possible to distinguish between the ANP receptor subtypes by the use of selective antagonists. Thus coinjection of ANP 102-121-des[Gln, Ser, Gly, Leu, Gly] (C-ANP), an ANP analog that selectively binds to the ANP C-receptor, decreased uptake in the kidneys by 50% but increased relative uptake in the lungs and soft tissues. This method permits for the first time, the dynamic in vivo analysis of ANP receptors and their interaction with endogenous ligand. Differences and changes in local ANP receptor concentrations and occupancy could be detected. Since ANP receptor density and affinity are influenced by various physiological and pathological conditions, clinical and diagnostic applications seem possible.
Willenbrock
R
R
Centre de Recherche, Hôtel-Dieu Hospital, University Montréal, Québec, Canada.
Lambert
R
R
Tremblay
J
J
Bavaria
G
G
Langlois
Y
Y
Léveillé
J
J
Flanagan
R
R
Hamet
P
P
D000818:Animals; D009320:Atrial Natriuretic Factor / Q000493:pharmacokinetics; D002522:Chlorocebus aethiops; D015902:Gamma Cameras; D007457:Iodine Radioisotopes; D007668:Kidney / Q000378:metabolism; D008168:Lung / Q000378:metabolism; D008297:Male; D017461:Receptors, Atrial Natriuretic Factor / Q000378:metabolism*
D007457:Iodine Radioisotopes; D009320:Atrial Natriuretic Factor; D017461:Receptors, Atrial Natriuretic Factor