The group A streptococcal M-type 3 protein gene exhibits a C terminus typical for class I M proteins.
The M protein gene (emm gene) from a reference group A streptococcal strain of serotype M3 was amplified by the polymerase chain reaction and partially sequenced. Hybridization assays using an oligonucleotide probe derived from the N-terminal sequence revealed that this gene segment is highly homologous among M-type 3 isolates. Of note, analysis of the nucleotide sequence data from the C terminus of the gene confirmed that the emm 3 gene exhibited all the features characteristic for group A streptococcal M-class I molecules. Recently published sequence data that were assigned to emm 3 resulted from a strain confusion and were shown to be the first one derived from an emm gene of an M-untypable isolate.
Podbielski
A
A
Institute of Medical Microbiology, Technical University (RWTH), Aachen, Federal Republic of Germany.
Baird
R
R
Kaufhold
A
A
D000595:Amino Acid Sequence; D000942:Antigens, Bacterial / Q000235:genetics; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D002352:Carrier Proteins; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment; D013297:Streptococcus pyogenes / Q000235:genetics*
D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D002352:Carrier Proteins; D004269:DNA, Bacterial; C014689:streptococcal M protein