title: Antisense oligonucleotide eliminates in vivo expression of c-fos in mammalian brain.
abstract: Immediate-early genes such as c-fos and NGFI-A are rapidly and transiently expressed in the striatum following amphetamine administration in vivo. Here we show that direct infusion of an antisense oligodeoxynucleotide to c-fos into striatum will reduce amphetamine-induced production of Fos-like immunoreactivity without affecting NGFI-A expression. These results suggest that it is possible to use antisense technology to study the role of immediate-early genes in specific sites in the brain in vivo.
authors.affiliation: Department of Pharmacology, Faculty of Medicine, Dalhousie University, Halifax, Nova Scotia, Canada.
authors.forename: B J
authors.initials: BJ
authors.lastname: Chiasson
authors.forename: M L
authors.initials: ML
authors.lastname: Hooper
authors.forename: P R
authors.initials: PR
authors.lastname: Murphy
authors.forename: H A
authors.initials: HA
authors.lastname: Robertson
mesh_terms: D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects; D016762:Genes, fos; D008297:Male; D008969:Molecular Sequence Data; D016376:Oligonucleotides, Antisense / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D016376:Oligonucleotides, Antisense