Analysis of the eicosapentaenoic acid (EPA) metabolite delta 17-6-keto-PGF1 alpha in human plasma by GC/SIM using [18O] delta 17-6-keto-PGF1 alpha.
A method of the microdetermination of delta 17-6-keto-PGF1 alpha, a hydrolyzed metabolite of PGI3, is described. An authentic delta 17-6-keto-PGF1 alpha (120 mg) was prepared from eicosapentaenoic acid (EPA) incubated with homogenate of bovine aortic intima. [18O]delta 17-6-Keto-PGF1 alpha was synthesized by repeating base-catalyzed hydrolysis of methyl ester derivatives in [18O]water, to obtain an internal standard in gas chromatography/selected ion monitoring (GC/SIM) of delta 17-6-keto-PGF1 alpha. Good linear response over the range of 10 pg-10ng was demonstrated. Chromatographic conditions using a MP-65HT column presented nearly baseline separation of delta 17-6-keto-PGF1 alpha and 6-keto-PGF1 alpha. We were able to detect delta 17-6-keto-PGF1 alpha in the range from 6 to 26 pg/ml of the human plasma. The present method can be applied to the determination of delta 17-6-keto-PGF1 alpha in the human urine and plasma.
Mizugaki
M
M
Department of Pharmaceutical Sciences, Tohoku University Hospital, Sendai, Japan.
Nishikawa
M
M
Hishinuma
T
T
Ohyama
Y
Y
Ishibashi
M
M
D015121:6-Ketoprostaglandin F1 alpha / Q000031:analogs & derivatives* / Q000097:blood; D000818:Animals; D001011:Aorta / Q000378:metabolism; D002417:Cattle; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D015118:Eicosapentaenoic Acid / Q000378:metabolism*; D006801:Humans; D008297:Male; D013058:Mass Spectrometry; D008832:Microchemistry; D010103:Oxygen Isotopes
D010103:Oxygen Isotopes; D015121:6-Ketoprostaglandin F1 alpha; C077778:delta(17)-6-ketoprostaglandin F1alpha; D015118:Eicosapentaenoic Acid