[The differential staining of murine metaphase chromosomes in early embryogenesis after treatment with restrictase AluI in situ].
The picture of differential staining of early mouse embryogenesis metaphasic chromosomes, from the first cleavage up to 10 days of gestation, after digestion by restriction endonuclease AluI was studied. It was shown that depending on the degree of digestion by endonuclease differential bandings of G+C- or C-type were observed. After the least digestion only the first cleavage chromosomes were differently stained. A slight difference in intensity of staining between paternal and maternal chromosomes of the zygote was observed. All the mouse chromosomes were identified after AluI digestion and staining after Giemsa.
Patkin
E L
EL
Sorokin
A V
AV
Kustova
M E
ME
D000818:Animals; D002871:Chromosome Banding / Q000379:methods*; D002875:Chromosomes / Q000187:drug effects* / Q000648:ultrastructure; D015252:Deoxyribonucleases, Type II Site-Specific / Q000494:pharmacology*; D005314:Embryonic and Fetal Development / Q000187:drug effects*; D008677:Metaphase / Q000187:drug effects*; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D013997:Time Factors; D015053:Zygote / Q000187:drug effects / Q000648:ultrastructure
C024669:endodeoxyribonuclease AluI; D015252:Deoxyribonucleases, Type II Site-Specific
Differentsial'noe okrashivanie metafaznykh khromosom myshi v rannem émbriogeneze posle obrabotki restriktazoĭ AluI in situ.