Specific anti-A responses of SCID mice populated with human lymphoid cells from peripheral blood, umbilical cord, bone marrow and spleen after immunization with group A erythrocytes [corrected].
Mice with severe combined immunodeficiency (SCID) were injected i.p. with 10 x 10(6) or 50 x 10(6) human leukocytes obtained from adult peripheral blood, umbilical cord blood, bone marrow and spleen from six group O individuals, together with allogeneic group A erythrocytes. Mice were bled every two weeks and levels of human IgG, IgM and anti-A were determined in the murine plasma. Spleen cells elicited the highest Ig levels (up to 5.2 mg/ml IgG) and umbilical cord the least (0-0.16 mg/ml IgG); maximum IgG levels were obtained at about 6-8 weeks after injection. Anti-A was detected in mice receiving adult peripheral blood or spleen leukocytes and immunizing erythrocytes 4-6 weeks after injection. Mice injected with the higher dose of leukocytes gave the best anti-A responses, but were more likely to develop tumours after 8 weeks.
Kumpel
B M
BM
U.K. Transplant Support Service, Bristol.
Poole
G D
GD
Jackson
D J
DJ
D000017:ABO Blood-Group System / Q000276:immunology*; D000372:Agglutination Tests; D000818:Animals; D001853:Bone Marrow / Q000276:immunology; D001854:Bone Marrow Cells; D004912:Erythrocytes / Q000276:immunology*; D005312:Fetal Blood / Q000166:cytology / Q000276:immunology; D006801:Humans; D007116:Immunization, Passive; D007074:Immunoglobulin G / Q000276:immunology; D007075:Immunoglobulin M / Q000276:immunology; D008214:Lymphocytes / Q000276:immunology*; D051379:Mice; D016513:Mice, SCID; D009374:Neoplasms, Experimental / Q000276:immunology; D016511:Severe Combined Immunodeficiency / Q000276:immunology*; D013154:Spleen / Q000166:cytology / Q000276:immunology; D014470:Umbilical Cord / Q000166:cytology / Q000276:immunology
D000017:ABO Blood-Group System; D007074:Immunoglobulin G; D007075:Immunoglobulin M