IGF-2 stimulates growth and metabolism of early mouse embryos.
Recent reports indicate that the insulin gene family plays a significant role in early development. Both insulin and IGF-1 stimulate growth and metabolism in preimplantation mouse embryos, however, little is known of the physiological effects of IGF-2. In this study, addition of IGF-2 to defined culture medium for the culture of 2-cell embryos stimulated blastocyst formation by 15%, ICM mitogenesis by 37%, and protein synthesis by 35%. EC50s of 12-63 pM IGF-2 for these responses were in the range for mediation by IGF-2 receptors. These results coupled with the previously demonstrated presence and expression of the IGF-2 receptor from the 2-cell stage supports a role for this third member of the insulin gene family in early development.
Harvey
M B
MB
Department of Physiology and Pharmacology, University of Queensland, Brisbane, Australia.
Kaye
P L
PL
D000818:Animals; D001755:Blastocyst / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D047108:Embryonic Development / Q000187:drug effects*; D005260:Female; D066298:In Vitro Techniques; D007335:Insulin-Like Growth Factor II / Q000494:pharmacology*; D051379:Mice; D008815:Mice, Inbred Strains; D008938:Mitosis / Q000187:drug effects; D011247:Pregnancy; D017527:Receptor, IGF Type 2 / Q000096:biosynthesis
D017527:Receptor, IGF Type 2; D007335:Insulin-Like Growth Factor II