title: Evaluation of the efficacy of d-penicillamine and trientine as copper chelators using an in vitro technique involving ovine red blood cells.
abstract: An in vitro technique for haemolysing ovine red blood cells with copper sulphate was standardized to induce c 50% haemolysis with 0.5 mM CuSO4 after incubation for 14 h at 38 degrees C. This technique was then applied to test the efficacy of trientine and d-penicillamine in preventing haemolysis. Trientine concentrations of 0.5; 1.0 and 1.5 mM were found to be the most effective (P < 0.05) in reducing copper-induced haemolysis. One and 1.5 mM concentrations of d-penicillamine were also effective (P < 0.05), but in this experiment a 0.5 mM concentration failed to protect the erythrocytes.
authors.affiliation: Department of Pharmacology and Toxicology, Faculty of Veterinary Science, University of Pretoria, Onderstepoort, Republic of South Africa.
authors.forename: C J
authors.initials: CJ
authors.lastname: Botha
authors.forename: T W
authors.initials: TW
authors.lastname: Naude
authors.forename: G E
authors.initials: GE
authors.lastname: Swan
authors.forename: A J
authors.initials: AJ
authors.lastname: Guthrie
mesh_terms: D000818:Animals; D003300:Copper / Q000633:toxicity*; D004912:Erythrocytes / Q000187:drug effects*; D005260:Female; D006461:Hemolysis / Q000187:drug effects*; D066298:In Vitro Techniques; D010396:Penicillamine / Q000627:therapeutic use*; D012756:Sheep / Q000097:blood*; D014266:Trientine / Q000627:therapeutic use*
chemical_list: D003300:Copper; D010396:Penicillamine; D014266:Trientine