Growth effects of tamoxifen on Lovo colon carcinoma cells and cultured cells from normal colonic mucosa.
The growth effects of tamoxifen (TAM) were studied using normal and malignant colonic epithelial cells. Addition of TAM (0.8 to 10 microM) to cultured normal human colon epithelial cells and Lovo colon adenocarcinoma cells produced a dose response decrease in growth of 42 to 76%. Histamine (1 to 10 microM) did not affect cell growth and did not negate the TAM effect. Calmodulin (2 micrograms/ml) totally blocked growth inhibitory effects of a calmodulin antagonist, trifluoperazine (10 microM), but did not block the TAM inhibitory effect. These data suggest that antiestrogen binding sites (AEBS) may play an important role in the growth-inhibitory effects of TAM on colon cells. Competition with estrogen and antagonism with histamine or calmodulin do not appear to be significant in this regard.
Lointier
P
P
Section of Gastrointestinal Oncology and Digestive Diseases, University of Texas M.D. Anderson Cancer Center, Houston 77030.
Wildrick
D M
DM
Boman
B M
BM
D000230:Adenocarcinoma / Q000473:pathology*; D002147:Calmodulin / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002927:Cimetidine / Q000494:pharmacology; D003106:Colon; D003110:Colonic Neoplasms / Q000473:pathology*; D004305:Dose-Response Relationship, Drug; D004847:Epithelial Cells; D004848:Epithelium / Q000187:drug effects; D004965:Estrogen Antagonists / Q000494:pharmacology; D006632:Histamine / Q000494:pharmacology; D006801:Humans; D007413:Intestinal Mucosa / Q000166:cytology / Q000187:drug effects*; D007700:Kinetics; D013629:Tamoxifen / Q000031:analogs & derivatives / Q000494:pharmacology*; D014268:Trifluoperazine / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D002147:Calmodulin; D004965:Estrogen Antagonists; D013629:Tamoxifen; C016601:afimoxifene; D014268:Trifluoperazine; D002927:Cimetidine; D006632:Histamine