title: Expression of the non-proliferation-specific protein, statin, in grey matter neuroglia of the aging rat brain.
abstract: The monoclonal antibody, S-44, identifies statin, a 57 kDa nuclear protein which appears to be expressed exclusively in non-proliferating cells. We previously demonstrated that in the aging rat corpus callosum approximately one third of neuroglia are statin-negative, suggesting the existence of an unexpectedly large cycling glial compartment. In the present study, double-labeling of individual cultured astroglia with [3H]thymidine and the S-44 antibody provided direct evidence for the non-proliferative status of statin-positive cells. The S-44 antibody was used to immuno-localize statin and thereby determine growth fractions for neuroglia in various grey matter regions of 3-, 18-, and 33-month-old rats. The proportion of statin-negative (cycling) cells for the three ages combined ranged from about 24% in the molecular layer of the dentate gyrus to 38% in the molecular layer of the parietal cortex. In most regions surveyed total glial counts and proportions of statin-positive and -negative cells did not vary significantly as a function of advancing age. These results suggest that (i) as in corpus callosum, pools of cycling neuroglia in various grey matter regions are far in excess of those previously predicted by S-phase labeling with [3H]thymidine or BUdR, and (ii) ratios of proliferating-to-quiescent neuroglia are tightly regulated over much of the animal's adult life span. These conserved ratios may be used as markers of normal CNS senescence, and deviations thereof may indicate the presence and extent of intervening neuropathologic processes.
authors.affiliation: Bloomfield Centre for Research in Aging, Lady Davis Institute for Medical Research, Sir Mortimer B. Davis-Jewish General Hospital, Montreal, Que., Canada.
authors.forename: H M
authors.initials: HM
authors.lastname: Schipper
authors.forename: R
authors.initials: R
authors.lastname: Mauricette
authors.forename: J J
authors.initials: JJ
authors.lastname: Liang
authors.forename: M J
authors.initials: MJ
authors.lastname: Lee
authors.forename: E
authors.initials: E
authors.lastname: Wang
mesh_terms: D000375:Aging / Q000378:metabolism*; D000818:Animals; D001345:Autoradiography; D001923:Brain Chemistry / Q000502:physiology*; D018797:Cell Cycle Proteins; D002455:Cell Division / Q000502:physiology; D005455:Fluorescent Antibody Technique; D007124:Immunoenzyme Techniques; D008297:Male; D009410:Nerve Degeneration / Q000502:physiology; D009419:Nerve Tissue Proteins / Q000032:analysis*; D009457:Neuroglia / Q000737:chemistry*; D009687:Nuclear Proteins / Q000032:analysis*; D011506:Proteins / Q000032:analysis*; D051381:Rats; D011916:Rats, Inbred F344
chemical_list: D018797:Cell Cycle Proteins; D009419:Nerve Tissue Proteins; D009687:Nuclear Proteins; D011506:Proteins