title: Lytic effect of Bacillus subtilis elastase on gram-positive and negative bacteria.
abstract: Elastase of B. subtilis 6a caused lysis of freshly grown cells of Gram-negative (Proteus vulgaris, Klebsiella pneumoniae, Salmonella typhi and Pseudomonas aeruginosa and Gram-positive (B. subtilis) bacteria. Heat killed and lyophilised Gram-positive and negative bacteria showed higher sensitivity to elastase. Both Gram-negative and Gram-positive bacteria were lysed maximally by elastase at pH 8.0. At this pH, activity of elastase was maximum in Tris-HCl and glycine-NaOH buffers followed by Tris-maleate and cacodylate buffers.
authors.affiliation: Department of Microbiology, Panjab University, Chandigarh, India.
authors.forename: M
authors.initials: M
authors.lastname: Gupta
authors.forename: M
authors.initials: M
authors.lastname: Kaur
authors.forename: K G
authors.initials: KG
authors.lastname: Gupta
mesh_terms: D001412:Bacillus subtilis / Q000201:enzymology*; D001426:Bacterial Proteins / Q000494:pharmacology*; D001433:Bacteriolysis; D006090:Gram-Negative Bacteria / Q000187:drug effects*; D006094:Gram-Positive Bacteria / Q000187:drug effects*; D006863:Hydrogen-Ion Concentration; D010196:Pancreatic Elastase / Q000494:pharmacology*
chemical_list: D001426:Bacterial Proteins; D010196:Pancreatic Elastase