Alternative splicing generates metabotropic glutamate receptors inducing different patterns of calcium release in Xenopus oocytes.
A splice variant of the metabotropic glutamate receptor (mGluR) 1a, named mGluR1c, was isolated. Compared to mGluR1a, the predicted mGluR1c protein is 302 amino acids shorter at its C-terminal end. Despite this difference, mGluR1c activates phospholipase C in Xenopus oocytes with a pharmacological profile identical to that of mGluR1a. However, in contrast to the large fast transient responses induced by mGluR1a, mGluR1c receptors elicit a small more slowly generated long-lasting oscillatory current, suggesting that these two receptors do not generate the same pattern of Ca2+ release in Xenopus oocytes. In situ hybridization data show that mGluR1c mRNA is expressed at a lower level than the other splice variants of mGluR1. Some differences in the regional distribution of these transcripts were observed in the cerebellum, the olfactory bulb, and the striatum.
Pin
J P
JP
Molecular Neurobiology Laboratory, Salk Institute for Biological Studies, San Diego, CA 92138-5800.
Waeber
C
C
Prezeau
L
L
Bockaert
J
J
Heinemann
S F
SF
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001224:Aspartic Acid / Q000494:pharmacology; D001483:Base Sequence; D002118:Calcium / Q000378:metabolism*; D003515:Cycloleucine / Q000031:analogs & derivatives / Q000494:pharmacology; D004533:Egtazic Acid / Q000494:pharmacology; D015723:Gene Library; D005971:Glutamates / Q000494:pharmacology; D007051:Ibotenic Acid / Q000494:pharmacology; D007608:Kainic Acid / Q000494:pharmacology; D008564:Membrane Potentials / Q000187:drug effects; D008969:Molecular Sequence Data; D009498:Neurotoxins / Q000494:pharmacology*; D009838:Oligodeoxyribonucleotides; D015345:Oligonucleotide Probes; D009865:Oocytes / Q000378:metabolism / Q000502:physiology*; D016133:Polymerase Chain Reaction; D017470:Receptors, Glutamate / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D015183:Restriction Mapping; D014981:Xenopus
D005971:Glutamates; D009498:Neurotoxins; D009838:Oligodeoxyribonucleotides; D015345:Oligonucleotide Probes; D017470:Receptors, Glutamate; D003515:Cycloleucine; C036361:1-amino-1,3-dicarboxycyclopentane; D007051:Ibotenic Acid; D001224:Aspartic Acid; D004533:Egtazic Acid; D007608:Kainic Acid; D002118:Calcium