Single-stranded DNA of 5'-upstream region of the rolC gene interacts with nuclear proteins of carrot cell cultures.
Using the gel retardation assay, proteins of carrot cells capable of binding to a single-stranded DNA of 5'-upstream region of the rolC gene were found. From competition experiments, these DNA-protein interactions were specific to single-stranded nucleotide sequence of Ava S fragment (from -94 bp to +23 bp relative to the transcription initiation site). Methylation interference experiments showed that G residue at the position of -41 bases on the bottom strand was important for DNA-protein binding. This residue was located between CAAT box and TATAA box. Such specific interaction between single-stranded DNA and nuclear proteins may play a role in transcription by RNA polymerase II.
Suzuki
A
A
Department of Botany, Faculty of Science, Hokkaido University, Sapporo, Japan.
Kato
A
A
Uchimiya
H
H
D001483:Base Sequence; D001667:Binding, Competitive; D002478:Cells, Cultured; D004247:DNA / Q000235:genetics / Q000378:metabolism*; D015342:DNA Probes; D004277:DNA, Single-Stranded / Q000235:genetics*; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000302:isolation & purification / Q000378:metabolism*; D010944:Plants / Q000235:genetics* / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D015183:Restriction Mapping; D014158:Transcription, Genetic
D015342:DNA Probes; D004277:DNA, Single-Stranded; D009687:Nuclear Proteins; D004247:DNA