Inhibition of human T-lymphocyte activation by macrolide antibiotic, roxithromycin.
Effects of macrolide antibiotic, roxithromycin (RXM) on human lymphocytes in culture were studied. The drug showed a dose-dependent inhibition of 3H-thymidine and 35S-methionine uptake responding to T cell mitogens and purified protein derivative of tuberculin (PPD). Activation by PPD, as assessed by 3H-thymidine uptake, was more sensitive to inhibition than the response to T cell mitogens. The drug produced a loss of blasts when added soon after transformation commenced. Immunosuppressive effects of RXM were further characterized by using four different types of metabolized RXM, RU 28111, RU 39001, RU 44981 and RU 45179. The most potent inhibitor of lymphocyte transformation was RU 45179, followed by RU 44981, RU 39001 and RU 28111 have little activity.
Konno
S
S
First Department of Internal Medicine, Showa University, Tokyo, Japan.
Adachi
M
M
Asano
K
K
Okamoto
K
K
Takahashi
T
T
D002478:Cells, Cultured; D004261:DNA Replication / Q000187:drug effects; D006801:Humans; D008213:Lymphocyte Activation / Q000187:drug effects* / Q000276:immunology; D008934:Mitogens / Q000494:pharmacology; D015575:Roxithromycin / Q000031:analogs & derivatives / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000187:drug effects* / Q000276:immunology
D008934:Mitogens; D015575:Roxithromycin