Zn(2+)-heparin interaction studied by potentiometric titration.
Measurement of the decrease in pH that accompanies the addition of Zn2+ to heparin in solution provided an indirect method of examining cation-polyanion interaction. Construction of plots analogous to isothermal saturation binding plots revealed the existence, for defined conditions of interaction, of a [heparin]-independent direct proportionality between the fraction of the maximal pH change occurring and the [Zn2+]/[heparin disaccharide] ratio. This accords with results from polarimetric examination of Ca(2+)- and Cu(2+)-heparin interactions. It suggests that, under the conditions used, cation-heparin interaction may result in the formation of a complex that exists in a colloid-like phase, between which and the aqueous phase, exchange of cations does not follow simple solution-phase reversible equilibrium thermodynamic behaviour. The results suggest that the putative Zn(2+)-containing complex is less stable in the presence of NaCl than is the corresponding Ca(2+)-containing complex. Addition of Zn2+ to low concentrations of heparins is accompanied by the usual decrease in pH, followed by a removal of H+ from solution as the [Zn2+]/[heparin disaccharide] ratio increases, suggesting dissolution of the putative complex. This reversal of the initial pH change was not seen for most other cation-heparin interactions under the conditions studied.
Grant
D
D
Department of Molecular and Cell Biology, University of Aberdeen, Marischal College, Scotland, U.K.
Long
W F
WF
Williamson
F B
FB
D001665:Binding Sites; D006493:Heparin / Q000378:metabolism*; D011199:Potentiometry; D015032:Zinc / Q000378:metabolism*
D006493:Heparin; D015032:Zinc