title: Stereospecific hydroxylation of indan by Escherichia coli containing the cloned toluene dioxygenase genes from Pseudomonas putida F1.
abstract: Escherichia coli JM109(pDTG601), containing the todC1C2BA genes encoding toluene dioxygenase from Pseudomonas putida F1, oxidizes indan to (-)-(1R)-indanol (83% R) and trans-1,3-indandiol. Under similar conditions, P. putida F39/D oxidizes indan to (-)-(1R)-indanol (96% R), 1-indanone, and trans-1,3-indandiol. The differences in the enantiomeric composition of the 1-indanols formed by the two organisms are due to the presence of a 1-indanol dehydrogenase in P. putida F39/D that preferentially oxidizes (+)-(1S)-indanol.
authors.affiliation: Department of Microbiology, University of Iowa, Iowa City 52242.
authors.forename: J M
authors.initials: JM
authors.lastname: Brand
authors.forename: D L
authors.initials: DL
authors.lastname: Cruden
authors.forename: G J
authors.initials: GJ
authors.lastname: Zylstra
authors.forename: D T
authors.initials: DT
authors.lastname: Gibson
mesh_terms: D003001:Cloning, Molecular; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics*; D006900:Hydroxylation; D007189:Indans / Q000737:chemistry / Q000378:metabolism*; D010105:Oxygenases / Q000235:genetics / Q000378:metabolism*; D016958:Pseudomonas putida / Q000235:genetics*; D013237:Stereoisomerism
chemical_list: D007189:Indans; D010105:Oxygenases; C023172:toluene dioxygenase