[Change in the cleavage site of synthetic substrates of SsoII restriction endonucleases upon introduction of non-nucleotide inserts in the recognition segment].
The cleavage of synthetic DNA duplexes containing 1,3-propanediol, 1,2-dideoxy-D-ribofuranose or 9-[1'-hydroxy-2'-(hydroxymethyl)ethoxy]methylguanine (glG) residues instead of one of dG residues or one of the nucleosides of the central base pair of the recognition site by SsoII restriction endonuclease (decreases CCNGG) has been studied. It is found that the non-nucleotide insertions (except for glG) result in a change of the SsoII cleavage site and an increase of the efficiency of the cleavage. The novel noncanonical cleavage occurs at the phosphodiester bond adjoining the non-nucleotide insert from the 5'-end.
Kubareva
E A
EA
Piatrauskene
O V
OV
Kariagina
A S
AS
Nikol'skaia
I I
II
Gromova
E S
ES
D001345:Autoradiography; D001483:Base Sequence; D004247:DNA / Q000737:chemistry; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism*; D004586:Electrophoresis; D008969:Molecular Sequence Data; D009692:Nucleic Acid Heteroduplexes / Q000737:chemistry; D013379:Substrate Specificity
D009692:Nucleic Acid Heteroduplexes; D004247:DNA; C045297:CCNGG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific
Izmenenie mesta rasshchepleniia sinteticheskikh substratov éndonukleazoĭ restriktsii SsoII pri vvedenii nenudleotidnykh vstavok v uchastok uznavaniia.