Purification and characterization of bovine brain gamma-aminobutyraldehyde dehydrogenase.
gamma-Aminobutyraldehyde dehydrogenase was purified to homogeneity from bovine brain. The molecular weight of the native enzyme and subunit were 230,000 and 58,000, respectively. The Km value for gamma-aminobutyraldehyde and NAD+ were 154 microM and 53 microM, respectively. The optimum pH and temperature were 8.0 and 37 degrees C, respectively. N-terminal sequence of the enzyme is as follows: NH2-S-A-A-T-Q-A-V-P-T-P-N-Q-Q-COOH. The enzyme migrates on isoelectric focusing with pI = 6.5. Enhancement of the enzyme activity by polyamine, Mn2+, Mg2+ and inhibition by gamma-aminobutyric acid and Zn2+ will enhance the limited information on regulation of the gamma-ABALDH activity and GABA metabolism to some extent.
Lee
J E
JE
Department of Biochemistry, College of Science, Yonsei University, Seoul, Korea.
Cho
Y D
YD
D000445:Aldehyde Oxidoreductases / Q000302:isolation & purification* / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000201:enzymology*; D002417:Cattle; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004221:Disulfiram / Q000494:pharmacology; D004950:Esterases / Q000302:isolation & purification / Q000378:metabolism; D007525:Isoelectric Focusing; D007700:Kinetics; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011073:Polyamines / Q000494:pharmacology; D013379:Substrate Specificity
D011073:Polyamines; D000445:Aldehyde Oxidoreductases; C019679:aminobutyraldehyde dehydrogenase; D004950:Esterases; D004221:Disulfiram