title: A GDP dissociation inhibitor that serves as a GTPase inhibitor for the Ras-like protein CDC42Hs.
abstract: Members of the family of Ras-related guanosine triphosphate (GTP) binding proteins appear to take part in the regulation of a number of biological processes, including cell growth and differentiation. Three different classes of proteins that regulate the GTP binding and GTP hydrolytic activities of the Ras family members have been identified. These different regulatory proteins inhibit guanosine diphosphate (GDP) dissociation (designated as GDIs), stimulate GDP dissociation and GDP-GTP exchange (designated as GDSs), or stimulate GTP hydrolysis (designated as GAPs). In the case of the Ras-like protein CDC42Hs, which is the human homolog of a Saccharomyces cerevisiae cell division cycle protein, the GDI protein also inhibited both the intrinsic and GAP-stimulated hydrolysis of GTP. These findings establish an additional role for the GDI protein--namely, as a guanosine triphosphatase (GTPase) inhibitory protein for a Ras-like GTP binding protein.
authors.affiliation: Department of Biochemistry, Cell, and Molecular Biology, Cornell University, Ithaca, NY 14853.
authors.forename: M J
authors.initials: MJ
authors.lastname: Hart
authors.forename: Y
authors.initials: Y
authors.lastname: Maru
authors.forename: D
authors.initials: D
authors.lastname: Leonard
authors.forename: O N
authors.initials: ON
authors.lastname: Witte
authors.forename: T
authors.initials: T
authors.lastname: Evans
authors.forename: R A
authors.initials: RA
authors.lastname: Cerione
mesh_terms: D000818:Animals; D001792:Blood Platelets / Q000737:chemistry; D001923:Brain Chemistry; D002417:Cattle; D004926:Escherichia coli / Q000235:genetics; D020558:GTP Phosphohydrolases / Q000037:antagonists & inhibitors*; D019204:GTP-Binding Proteins / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D020690:GTPase-Activating Proteins; D020730:Guanine Nucleotide Dissociation Inhibitors; D016244:Guanosine 5'-O-(3-Thiotriphosphate) / Q000378:metabolism; D006153:Guanosine Diphosphate / Q000378:metabolism; D006160:Guanosine Triphosphate / Q000378:metabolism; D006868:Hydrolysis; D008565:Membrane Proteins / Q000378:metabolism; D015513:Oncogene Proteins / Q000378:metabolism; D011505:Protein-Tyrosine Kinases; D011506:Proteins / Q000378:metabolism; D011518:Proto-Oncogene Proteins; D051562:Proto-Oncogene Proteins c-bcr; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D020764:cdc42 GTP-Binding Protein; D020703:ras GTPase-Activating Proteins; D062947:rho-Specific Guanine Nucleotide Dissociation Inhibitors; D020743:rhoB GTP-Binding Protein
chemical_list: D020690:GTPase-Activating Proteins; D020730:Guanine Nucleotide Dissociation Inhibitors; D008565:Membrane Proteins; D015513:Oncogene Proteins; D011506:Proteins; D011518:Proto-Oncogene Proteins; D011993:Recombinant Fusion Proteins; D020703:ras GTPase-Activating Proteins; D062947:rho-Specific Guanine Nucleotide Dissociation Inhibitors; D006153:Guanosine Diphosphate; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D006160:Guanosine Triphosphate; D011505:Protein-Tyrosine Kinases; D051562:Proto-Oncogene Proteins c-bcr; D020558:GTP Phosphohydrolases; D019204:GTP-Binding Proteins; D020764:cdc42 GTP-Binding Protein; D020743:rhoB GTP-Binding Protein