Cometabolic degradation of chlorinated alkenes by alkene monooxygenase in a propylene-grown Xanthobacter strain.
Propylene-grown Xanthobacter cells (strain Py2) degraded several chlorinated alkenes of environmental concern, including trichloroethylene, 1-chloroethylene (vinyl chloride), cis- and trans-1,2-dichloroethylene, 1,3-dichloropropylene, and 2,3-dichloropropylene. 1,1-Dichloroethylene was not degraded efficiently, while tetrachloroethylene was not degraded. The role of alkene monooxygenase in catalyzing chlorinated alkene degradations was established by demonstrating that glucose-grown cells which lack alkene monooxygenase and propylene-grown cells in which alkene monooxygenase was selectively inactivated by propyne were unable to degrade the compounds. C2 and C3 chlorinated alkanes were not oxidized by alkene monooxygenase, but a number of these compounds were inhibitors of propylene and ethylene oxidation, suggesting that they compete for binding to the enzyme. A number of metabolites enhanced the rate of degradation of chlorinated alkenes, including propylene oxide, propionaldehyde, and glucose. Propylene stimulated chlorinated alkene oxidation slightly when present at a low concentration but became inhibitory at higher concentrations. Toxic effects associated with chlorinated alkene oxidations were determined by measuring the propylene oxidation and propylene oxide-dependent O2 uptake rates of cells previously incubated with chlorinated alkenes. Compounds which were substrates for alkene monooxygenase exhibited various levels of toxicity, with 1,1-dichloroethylene and trichloroethylene being the most potent inactivators of propylene oxidation and 1,3- and 2,3-dichloropropylene being the most potent inactivators of propylene oxide-dependent O2 uptake. No toxic effects were seen when cells were incubated with chlorinated alkenes anaerobically, indicating that the product(s) of chlorinated alkene oxidation mediates toxicity.
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Laboratory for Nitrogen Fixation Research, Oregon State Univrsity, Corvallis 97331-2902.
Hyman
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D000475:Alkenes / Q000378:metabolism* / Q000494:pharmacology; D001673:Biodegradation, Environmental / Q000187:drug effects; D004852:Epoxy Compounds / Q000378:metabolism; D005030:Ethylenes / Q000494:pharmacology; D006088:Gram-Negative Aerobic Bacteria / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D006843:Hydrocarbons, Chlorinated / Q000378:metabolism* / Q000494:pharmacology; D010084:Oxidation-Reduction / Q000187:drug effects; D010100:Oxygen / Q000378:metabolism; D010105:Oxygenases / Q000378:metabolism* / Q000494:pharmacology
D000475:Alkenes; D004852:Epoxy Compounds; D005030:Ethylenes; D006843:Hydrocarbons, Chlorinated; C036216:ethylene; C013658:propylene; D010105:Oxygenases; C072883:alkene monooxygenase; D010100:Oxygen; C009068:propylene oxide