Experimental evaluation of antitoxic protective effect of new cholera vaccines in mice.
Intraperitoneal immunization of mice and subsequent challenge with purified cholera toxin (CT) were employed to evaluate the anti-cholera toxin protective effect of two new oral cholera vaccines, live CVD 103-HgR and killed B subunit-whole cell (BS-WC). CVD 103-HgR vaccine demonstrated 100% protection of mice against 2.25 LD50 and 70% against 3 LD50 of CT. Mice immunized with BS-WC vaccine were protected against 2.25 and 3 LD50 of CT in 88 and 62% of cases, respectively. All three killed parenteral vaccines failed to protect against CT. We suggest this mouse system for preliminary evaluation of the antitoxic protective activity of cholera vaccines.
Dragunsky
E M
EM
Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, MD 20892.
Rivera
E
E
Aaronson
W
W
Dolgaya
T M
TM
Hochstein
H D
HD
Habig
W H
WH
Levenbook
I S
IS
D000818:Animals; D000992:Antitoxins / Q000627:therapeutic use*; D002771:Cholera / Q000517:prevention & control*; D002772:Cholera Toxin / Q000008:administration & dosage / Q000037:antagonists & inhibitors*; D022121:Cholera Vaccines / Q000627:therapeutic use*; D004353:Drug Evaluation, Preclinical; D007114:Immunization; D007274:Injections, Intraperitoneal; D007928:Lethal Dose 50; D008297:Male; D051379:Mice
D000992:Antitoxins; D022121:Cholera Vaccines; D002772:Cholera Toxin