Modification of the DiSC assay by the incorporation of monoclonal antibody staining.
The use of immunohistochemical staining with the DiSC assay is described. This modified staining procedure allows differentiation of viable human tumor cells from normal cells in biopsy preparations, facilitating the evaluation of slides from chemosensitivity assays. Samples from ALL and CLL patients were processed using both the DiSC and modified MoAb-DiSC procedure. The predictive results were compared and found to be identical. This procedure is advantageous in determining the chemosensitivity of tumor cells in a highly heterogeneous biopsy specimen.
Kirkpatrick
D L
DL
Department of Chemistry, University of Regina, Saskatchewan, Canada.
Chen
L
L
Goh
T S
TS
D000911:Antibodies, Monoclonal; D004354:Drug Screening Assays, Antitumor / Q000379:methods*; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*
D000911:Antibodies, Monoclonal