Cloning and characterization of a Neisseria gene homologous to hisJ and argT of Escherichia coli and Salmonella typhimurium.
We have isolated from a genomic library of the pathogenic Neisseriae gonorrhoeae T2 strain, a gene encoding a putative protein of 268 amino acids which exhibited significant similarity to the hisJ and argT gene products of Salmonella typhimurium and Escherichia coli, periplasmic proteins deputed to amino acid transport within the cell. The gene is transcribed as a monocistronic mRNA species of about 960 nucleotides flanked by regulatory elements for initiation and termination of transcription that are efficiently recognized in an E. coli host.
Lavitola
A
A
Dipartimento di Biologia e Patologia Cellulare e Molecolare, Università di Napoli, Italy.
Vanni
M
M
Martin
P M
PM
Bruni
C B
CB
D000596:Amino Acids / Q000493:pharmacokinetics; D001426:Bacterial Proteins / Q000235:genetics*; D001693:Biological Transport, Active / Q000502:physiology; D015152:Blotting, Northern; D015139:Blotting, Southern; D015153:Blotting, Western; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics*; D066298:In Vitro Techniques; D009344:Neisseria gonorrhoeae / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D015183:Restriction Mapping; D012486:Salmonella typhimurium / Q000235:genetics*
D000596:Amino Acids; D001426:Bacterial Proteins