Analysis of the biogenic amines in the central nervous system of the tobacco hornworm by high-performance liquid chromatography with 16-sensor electrochemical detection.
A method was developed to analyze biogenic amines in extracts of the central nervous system of the tobacco hornworm, Manduca sexta by high-performance liquid chromatography with 16-sensor electrochemical detection (n-EC-HPLC). The amines, precursors, and metabolites were separated in two dimensions. The first dimension involves separation based upon retention time by reversed-phase HPLC, while the second dimension involves separation based upon the characteristic oxidation potentials achieved by n-EC. Biogenic amine identification was based upon maximum oxidation potential and peak height ratios in addition to retention time. The improved resolving power of this method allows for a simplified sample preparation procedure and simultaneous determination of a wide range of compounds, including phenylethylamine, catecholamines, indoleamines, and some of their precursors and metabolites.
Sparks
T C
TC
DowElanco Discovery Research, Insect Biochemistry Laboratory, Greenfield, Indiana 46140.
Geng
C
C
D000818:Animals; D001679:Biogenic Amines / Q000032:analysis*; D002490:Central Nervous System / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004563:Electrochemistry; D009036:Moths
D001679:Biogenic Amines