Induction of Krox-20 expression after focal cerebral ischemia.
Krox-20 is one of the transcription factors of "zinc finger" family. The expression of Krox-20 was investigated in a rat focal cerebral ischemia model by Northern blot analysis and in situ hybridization. Northern blot analysis showed that ischemia for 30 min which caused little cortical infarction induced a 29-fold increase in Krox-20 mRNA signal exclusively in the ischemic cortex. Ischemia for 90 min which led to large cortical infarction induced Krox-20 mRNA not only in the ischemic cortex but also in the ipsilateral hippocampus. The induction of Krox-20 mRNA was rapid, transient and appeared to be controlled at the transcriptional level, as indicated by nuclear run-on assays. The regional induction of Krox-20 mRNA was further confirmed by in situ hybridization. These results suggest that the expression of transcription factor genes may play a role in the post-ischemic changes of the injured brain.
An
G
G
Division of Restorative Neurology, Baylor College of Medicine, Houston, TX 77030.
Lin
T N
TN
Liu
J S
JS
Hsu
C Y
CY
D002545:Brain Ischemia / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism; D002540:Cerebral Cortex / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000096:biosynthesis*; D051767:Early Growth Response Protein 2; D006651:Histocytochemistry; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000032:analysis; D015427:Reperfusion Injury / Q000378:metabolism; D013997:Time Factors; D014157:Transcription Factors / Q000096:biosynthesis*; D014158:Transcription, Genetic; D016335:Zinc Fingers
D004268:DNA-Binding Proteins; D051767:Early Growth Response Protein 2; D012333:RNA, Messenger; D014157:Transcription Factors