Disruption of mitochondrial function by suramin measured by rhodamine 123 retention and oxygen consumption in intact DU145 prostate carcinoma cells.
Suramin, an antiparasitic drug, has shown antitumor activity in humans. This may occur in part through disruption of energy balance, which is believed to be part of its antiparasitic action. Suramin disrupts mitochondrial function in intact DU145 prostate carcinoma cell monolayers as seen by its causing the release of rhodamine 123 from prestained cells beginning at about 10 microM in 96-well microtiter plates measured with a fluorescent plate scanner. This effect was similar to the ionophore carbonyl cyanide m-chlorophenylhydrazone, dissolved in ethanol at 0.01 N and indicates that suramin acts as a respiratory poison or an ionophore. This effect was confirmed by studies of oxygen consumption with a Clark oxygen electrode and cellular ATP content which demonstrated uncoupling of oxidative phosphorylation by 100 microM suramin, a clinically achievable plasma drug level.
Rago
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William S. Middleton Veteran's Hospital, Madison, Wisconsin.
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Wilding
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D000255:Adenosine Triphosphate / Q000032:analysis; D005456:Fluorescent Dyes / Q000378:metabolism*; D006801:Humans; D008297:Male; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism; D010101:Oxygen Consumption; D011471:Prostatic Neoplasms / Q000378:metabolism*; D020112:Rhodamine 123; D012235:Rhodamines / Q000378:metabolism*; D013498:Suramin / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D005456:Fluorescent Dyes; D012235:Rhodamines; D020112:Rhodamine 123; D013498:Suramin; D000255:Adenosine Triphosphate