title: Molecular cloning of an alanine aminotransferase from NAD-malic enzyme type C4 plant Panicum miliaceum.
abstract: We have determined the nucleotide sequence of a cDNA encoding AlaAT-2, which is believed to function in the C4-pathway of Panicum miliaceum. An open reading frame (1446 bp) encodes a protein of 482 amino acid residues. The deduced amino acid sequence of AlaAT-2 shows 44.2 and 44.8% homology with the amino acid sequences of AlaATs from rat and human livers, respectively. Northern blot analysis showed that the gene encoding AlaAT-2 in mesophyll and bundle sheath cells was the same and transcribed similarly in the cells. The level of translatable mRNA for AlaAT-2 increased dramatically during greening.
authors.affiliation: Department of Agricultural Chemistry, School of Agriculture, Nagoya University, Japan.
authors.forename: D
authors.initials: D
authors.lastname: Son
authors.forename: T
authors.initials: T
authors.lastname: Sugiyama
mesh_terms: D000410:Alanine Transaminase / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004247:DNA / Q000302:isolation & purification; D006801:Humans; D008027:Light; D008291:Malate Dehydrogenase / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D009928:Organ Specificity / Q000235:genetics; D008897:Panicum / Q000201:enzymology / Q000235:genetics* / Q000528:radiation effects; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid
chemical_list: D004247:DNA; D008291:Malate Dehydrogenase; C046446:malate dehydrogenase-(oxaloacetate-decarboxylating) (NAD+); D000410:Alanine Transaminase