A sensitive method for the detection of immune complexes in human gingival crevicular fluid.
An assay for immune complexes [ICs] in gingival crevicular fluid [GCF] could be potentially useful to ascertain the risk of periodontal disease activity at specific oral sites. This paper describes and ELISA-based method sufficiently sensitive to measure ICs in GCF samples at levels below 1 microgram/ml. Static GCF samples taken from 5 adults showed IC levels ranging from 5 to 166 micrograms/ml. Additional tests of 10 GCF samples from 1 adult were conducted with complement components and indicated that either C1q or rabbit anti-human C3d was suitable as a capture agent in the assay procedure. Further application of this assay may help to assess the usefulness of IC levels in GCF samples as possible diagnostic indicators of periodontal disease activity.
Turner
D W
DW
Department of Periodontics, School of Dentistry, Northwestern University, Chicago, Illinois 60611-2909.
Pederson
E D
ED
Lamberts
B L
BL
D000328:Adult; D000936:Antigen-Antibody Complex / Q000032:analysis*; D015922:Complement C1q; D015933:Complement C3d; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D005883:Gingival Crevicular Fluid / Q000276:immunology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D010514:Periodontal Pocket / Q000276:immunology; D010518:Periodontitis / Q000276:immunology*
D000936:Antigen-Antibody Complex; D015922:Complement C1q; D015933:Complement C3d