c-fos mRNA constitutive expression by mature human megakaryocytes.
By in situ hybridization with a c-fos probe, we have shown that human bone marrow megakaryocytes cultured in the presence of 20% aplastic anemia plasma constitutively express c-fos mRNA. At day 0, megakaryocytes are mostly immature and only 3% of them are labeled. The number of labeled cells reached 23% after 12 days of culture. Interleukin 3 (IL-3) and IL-6 added together at day 10 further increased this number to 31% 2 days later. Mature labeled megakaryocytes were more numerous and more strongly labeled than immature ones. These results suggest that c-fos could play a role in megakaryocytic terminal differentiation, either in the polyploidization or in the thrombopoietic function unique to these cells.
Panterne
B
B
Laboratoire CNRS de Biologie Cellulaire et Moléculaire des Facteurs de Croissance, Hôpital Paul Brousse, Villejuif, France.
Hatzfeld
J
J
Blanchard
J M
JM
Levesque
J P
JP
Berthier
R
R
Ginsbourg
M
M
Hatzfeld
A
A
D016762:Genes, fos; D006801:Humans; D017403:In Situ Hybridization; D066298:In Vitro Techniques; D007377:Interleukin-3 / Q000494:pharmacology; D015850:Interleukin-6 / Q000494:pharmacology; D008533:Megakaryocytes / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis*
D007377:Interleukin-3; D015850:Interleukin-6; D012333:RNA, Messenger