Differential subcellular distribution of PC1, PC2 and furin in bovine adrenal medulla and secretion of PC1 and PC2 from this tissue.
The subcellular distribution of PC1, PC2 and furin was determined in bovine adrenal medulla by immunoblotting of fractions obtained by density gradient centrifugation. PC1 and PC2 were found to be confined to chromaffin granules whereas furin (C-terminal-peptide) was absent from these organelles. Stimulation of bovine adrenal medulla by carbamoylcholine chloride induced the secretion of PC1 and PC2. The secreted enzymes had the same molecular size as PC1 and PC2 present in chromaffin granules.
Kirchmair
R
R
Department of Pharmacology, University of Innsbruck, Austria.
Egger
C
C
Gee
P
P
Hogue-Angeletti
R
R
Fischer-Colbrie
R
R
Laslop
A
A
Winkler
H
H
D000313:Adrenal Medulla / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism / Q000648:ultrastructure; D000818:Animals; D002217:Carbachol / Q000494:pharmacology; D002417:Cattle; D002499:Centrifugation, Density Gradient; D002837:Chromaffin Granules / Q000201:enzymology* / Q000378:metabolism; D003594:Cytoplasmic Granules / Q000201:enzymology* / Q000378:metabolism; D045683:Furin; D008565:Membrane Proteins / Q000032:analysis* / Q000378:metabolism; D045706:Proprotein Convertase 2; D043484:Proprotein Convertases; D012697:Serine Endopeptidases / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D013381:Subtilisins / Q000032:analysis*
D008565:Membrane Proteins; D002217:Carbachol; D043484:Proprotein Convertases; D012697:Serine Endopeptidases; D013381:Subtilisins; D045683:Furin; D045706:Proprotein Convertase 2