IGF-II mRNA expression in LI human glioblastoma cell line parallels cell growth.
A human glioblastoma cell line was found to express in vitro mRNA transcripts specific for insulin-like growth factor-II (IGF-II) and growth-hormone releasing-hormone (GHRH). In the absence of gross morphological changes, retinoic acid reduced the growth rate without major change of IGF-II mRNA expression, while alpha-difluoromethylornithine produced a complete growth arrest and a sharp decrease of IGF-II mRNA expression. Both reagents increased the expression of GHRH mRNA. Also in this glioblastoma cell line, like other neuroectodermal tumours, IGF-II mRNA is expressed independently from GHRH and seems to be parallel to growth rate.
Melino
G
G
University of Rome Tor Vergata, Italy.
Stephanou
A
A
Annicchiarico-Petruzzelli
M
M
Finazzi-Agró
A
A
Knight
R A
RA
Lightman
S L
SL
D015152:Blotting, Northern; D001932:Brain Neoplasms / Q000378:metabolism*; D000518:Eflornithine / Q000494:pharmacology; D005910:Glioma / Q000378:metabolism*; D013007:Growth Hormone-Releasing Hormone / Q000378:metabolism; D006801:Humans; D007335:Insulin-Like Growth Factor II / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis* / Q000302:isolation & purification; D012334:RNA, Neoplasm / Q000032:analysis / Q000096:biosynthesis* / Q000302:isolation & purification; D014212:Tretinoin / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D012333:RNA, Messenger; D012334:RNA, Neoplasm; D014212:Tretinoin; D007335:Insulin-Like Growth Factor II; D013007:Growth Hormone-Releasing Hormone; D000518:Eflornithine