Variable effects of chemical allergens on serum IgE concentration in mice. Preliminary evaluation of a novel approach to the identification of respiratory sensitizers.
A wide variety of chemicals may induce allergic contact dermatitis (contact sensitivity). Some chemical allergens may, in addition, cause respiratory sensitization. Topical exposure of mice to contact and respiratory chemical sensitizers results in the initiation of divergent immune responses characteristic of preferential activation of different functional subpopulations of T helper (TH) cells. In the present study we have sought to make use of these differences, particularly differences in the ability of contact and respiratory allergens to provoke IgE responses, and to question whether opportunities exist for the identification of chemicals with the potential for respiratory sensitization. We have examined alterations in the serum concentration of IgE following topical exposure of mice to seven chemical allergens; trimellitic anhydride (TMA), phthalic anhydride, diphenylmethane-4,4'-diisocyanate (MDI), dicyclohexylmethane-4,4'-diisocyanate (HMDI), isophorone diisocyanate (IPDI), oxazolone and 2,4-dinitrochlorobenzene (DNCB). Three of these--TMA, phthalic anhydride and MDI--are known human respiratory sensitizers. The other four--HMDI, IPDI, oxazolone and DNCB--appear not to cause respiratory allergy, or at least have a very limited potential to do so. At the concentrations tested, exposure to all chemicals caused a lymphocyte proliferative response in lymph nodes draining the site of application. However, exposure only to TMA, phthalic anhydride and MDI resulted in a substantial increase in the concentration of serum IgE. Treatment with HMDI and IPDI failed to induce any change in serum IgE concentration. DNCB and oxazolone caused only small and transient elevations of IgE that were considerably less marked than those observed with respiratory sensitizers.(ABSTRACT TRUNCATED AT 250 WORDS)
Dearman
R J
RJ
ICI Central Toxicology Laboratory, Alderley Park, Macclesfield, UK.
Basketter
D A
DA
Kimber
I
I
D000485:Allergens / Q000633:toxicity*; D000818:Animals; D003485:Cyanates / Q000276:immunology / Q000633:toxicity; D003877:Dermatitis, Contact / Q000097:blood / Q000209:etiology / Q000276:immunology*; D004137:Dinitrochlorobenzene / Q000276:immunology / Q000633:toxicity; D005260:Female; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood*; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D010081:Oxazolone / Q000276:immunology / Q000633:toxicity; D010796:Phthalic Anhydrides / Q000276:immunology / Q000633:toxicity; D012130:Respiratory Hypersensitivity / Q000097:blood / Q000139:chemically induced / Q000276:immunology*
D000485:Allergens; D003485:Cyanates; D004137:Dinitrochlorobenzene; D010796:Phthalic Anhydrides; D010081:Oxazolone; D007073:Immunoglobulin E