[Use of water-soluble 2-nitro-4-sulfophenyl ester of adipic acid for preparation of peptide protein conjugates].
Two synthetic peptides were conjugated with bovine serum albumin by means of 2-nitro-4-sulfophenyl ester of adipic acid. The amino acid analysis of the conjugates has shown that 14-15 molecules of the peptide are coupled per 1 molecule of the albumin during 10 min. The number of coupled molecules is the same when the reaction time increases to 24 hours. So, 2-nitro-4-sulfophenyl ester of adipic acid may be used parallel with the known bifunctional reagents to obtain peptide-protein conjugates.
Zaĭtseva
L S
LS
Gershkovich
A A
AA
Radavskiĭ
Iu L
IuL
Abakumov
V Iu
VIu
D000272:Adipates; D000595:Amino Acid Sequence; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D012995:Solubility; D014867:Water / Q000737:chemistry*
D000272:Adipates; D010455:Peptides; D014867:Water; C077679:2-nitro-4-sulfophenyl adipate; D012710:Serum Albumin, Bovine
Primenenie vodorastvorimogo 2-nitro-4-sul'fofenilovogo efira adipinovoĭ kisloty dlia polucheniia kon"iugatov peptid-belok.