title: Evidence of a neurogenic component during IgE-mediated inflammation in mouse skin.
abstract: IgE-mediated inflammation was measured in mouse footpads that lacked sciatic innervation. Mice were passively sensitized with a monoclonal antibody, IgE anti-dinitrophenol, or were immunized for specific IgE production. Antigen-induced swelling in the denervated footpads was reduced 23-39% when compared to sham or untreated controls. Reduced IgE-mediated swelling responses were attributed to the loss of a mast cell-nerve interaction and not to blood vessel sensitivity to vasoamines. Furthermore, electrical stimulation of the distal segment of the sciatic nerve completely restored IgE-mediated inflammation. These data provide in vivo evidence that peripheral nerves participate in cutaneous IgE-mediated swelling reactions with the net effect of increasing inflammation.
authors.affiliation: Department of Biological Sciences, Old Dominion University, Norfolk, VA 23529-0266.
authors.forename: R E
authors.initials: RE
authors.lastname: Ratzlaff
authors.forename: V J
authors.initials: VJ
authors.lastname: Cavanaugh
authors.forename: G W
authors.initials: GW
authors.lastname: Miller
authors.forename: S G
authors.initials: SG
authors.lastname: Oakes
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D003714:Denervation; D003876:Dermatitis, Atopic / Q000276:immunology*; D004306:Dose-Response Relationship, Immunologic; D004558:Electric Stimulation; D005260:Female; D006632:Histamine / Q000276:immunology; D007111:Immunity, Cellular; D007116:Immunization, Passive; D007073:Immunoglobulin E / Q000276:immunology*; D008407:Mast Cells / Q000187:drug effects / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D012584:Sciatic Nerve / Q000502:physiology*; D012701:Serotonin / Q000276:immunology; D012710:Serum Albumin, Bovine / Q000494:pharmacology; D013997:Time Factors
chemical_list: D000911:Antibodies, Monoclonal; D012710:Serum Albumin, Bovine; D012701:Serotonin; D007073:Immunoglobulin E; D006632:Histamine