IGF-I binding and IGF-I expression in regenerating muscle of normal and hypophysectomized rats.
Binding of iodinated IGF-I to tissue sections from regenerating muscle was studied by autoradiography in normal and in hypophysectomized rats. Binding of IGF-I was low in control muscle in both groups of animals, but increased transiently about 10-fold during regeneration after injury. Maximal binding occurred later in hypophysectomized rats than in control rats, and there was also a slower regeneration process in these animals. IGF-I, as demonstrated by immunohistochemistry, and IGF-I mRNA, as demonstrated by in situ hybridization, were expressed by the regenerating muscle cells in both groups of animals. It is concluded that locally produced IGF-I is the most likely ligand for IGF-I receptors during muscle regeneration.
Jennische
E
E
Department of Histology, University of Göteborg, Sweden.
Matejka
G L
GL
D000818:Animals; D001345:Autoradiography; D015342:DNA Probes; D003720:Densitometry; D004073:Digitoxigenin; D007016:Hypophysectomy; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D007334:Insulin-Like Growth Factor I / Q000096:biosynthesis / Q000378:metabolism*; D007457:Iodine Radioisotopes; D008297:Male; D009132:Muscles / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D012038:Regeneration
D015342:DNA Probes; D007457:Iodine Radioisotopes; D012333:RNA, Messenger; D004073:Digitoxigenin; D007334:Insulin-Like Growth Factor I