Crystallization and preliminary X-ray investigation of phosphoribosylaminoimidazolesuccinocarboxamide synthase from the yeast Saccharomyces cerevisiae.
Crystals of phosphoribosylaminoimidazolesuccinocarboxamide synthase (EC 6.3.2.6) from the yeast Saccharomyces cerevisiae were grown by the vapor diffusion hanging-drop technique, using ammonium sulfate as the precipitant. The crystals had dimensions up to 1.2 mm. X-ray diffraction experiments indicated a space group of P2(1)2(1)2(1) and unit cell parameters of a = 62.3 A, b = 63.5 A and c = 80.9 A, with one molecule in the asymmetric unit. Native data have been collected to 2.5 A resolution.
Grebenko
A I
AI
Institute of Crystallography, Academy of Sciences of Russia, Moscow.
Levdikov
V M
VM
Barynin
V V
VV
Melik-Adamyan
W R
WR
Myasnikov
A N
AN
D003460:Crystallization; D010453:Peptide Synthases / Q000737:chemistry*; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D014961:X-Ray Diffraction
D010453:Peptide Synthases; C022379:phosphoribosylaminoimidazole-succinocarboxamide synthetase