c-myc and p53 gene expression in the differentiation of temperature-sensitive mutants of teratocarcinoma F9 cells.
We have previously reported the isolation of several temperature-sensitive (ts) mutants of F9 cells. Further investigations showed that some mutants were induced to differentiate at non-permissive temperature of cell growth, accompanied by changes in the expression of various genes, whereas others were not. During the differentiation induced by shifting up to the non-permissive temperature, a rapid and transient decrease in both c-myc and p53 mRNA levels and rapid induction of c-jun mRNA were observed. These changes were specific in differentiation-inducible mutants and were not observed in a non-inducible mutant. In both types of mutants, the level of c-myc mRNA decreased in association with growth retardation at the non-permissive temperature. The p53 mRNA, however, showed specific increase in the differentiation-inducible ts mutants. These observations suggest distinct roles for p53 and c-myc from proliferation to differentiation in teratocarcinoma stem cells.
Kosaka
M
M
Research Institute for Microbial Diseases, Dental Faculty, Osaka University, Japan.
Iwai
S A
SA
Nishina
Y
Y
Sumi
T
T
Nishimune
Y
Y
D000818:Animals; D015152:Blotting, Northern; D002454:Cell Differentiation / Q000235:genetics*; D002455:Cell Division; D015870:Gene Expression; D016758:Genes, jun; D016259:Genes, myc; D016158:Genes, p53; D007700:Kinetics; D051379:Mice; D009154:Mutation; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D012334:RNA, Neoplasm / Q000235:genetics / Q000302:isolation & purification; D013696:Temperature; D013724:Teratoma / Q000235:genetics* / Q000473:pathology; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured; D016159:Tumor Suppressor Protein p53 / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics
D012333:RNA, Messenger; D012334:RNA, Neoplasm; D016159:Tumor Suppressor Protein p53