Expression of subunit II of chloroplast H(+)-ATPase in an Escherichia coli mutant lacking subunit b of its H(+)-ATPase.
The DNA of subunit II of the H(+)-ATPase from spinach chloroplast was expressed in Escherichia coli. It was found that a high gene dose is lethal to E. coli. With a lower gene dose subunit II was not able to substitute for the homologous subunit b in the E. coli ATP synthase.
Schmidt
G
G
Biologisches Institut, Universität Stuttgart.
D002736:Chloroplasts / Q000201:enzymology*; D004247:DNA / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic; D017343:Genes, Plant; D009154:Mutation; D010944:Plants / Q000201:enzymology / Q000235:genetics; D010957:Plasmids; D006180:Proton-Translocating ATPases / Q000096:biosynthesis*
D004247:DNA; D006180:Proton-Translocating ATPases