Paramagnetic water proton relaxation enhancement: from contrast agents in MRI to reagents for quantitative "in vitro" assays.
The use of stable complexes of paramagnetic metal ions as probes in analytical applications of the NMR technique is discussed. Two examples are given that involve the determination of the total Fe3+ content of human serum transferrin (Tf) and the extent of glycation of human serum albumin (HSA). The method is based on the measurement of the longitudinal water proton relaxation times, conveniently altered following a selective chemical interaction between the substrate and the paramagnetic probe.
Aime
S
S
Dipartimento di Chimica Inorganica, Università degli Studi di Torino, Italy.
Botta
M
M
Ermondi
G
G
Fasano
M
M
Terreno
E
E
D003287:Contrast Media; D008279:Magnetic Resonance Imaging; D009682:Magnetic Resonance Spectroscopy; D014867:Water
D003287:Contrast Media; D014867:Water