title: The specificity and elastinolytic activities of bovine cathepsins S and H.
abstract: Cathepsins S and H were purified from bovine spleen and their catalytic properties compared. The enzymes were shown to be similar by chromatographic properties and by the ability to hydrolyze Bz-Phe-Val-Arg-NHMec. They could however be distinguished by the fact that cathepsin S reacted with Z-[125I]Tyr-Ala-CHN2 and hydrolyzed Z-Phe-Arg-NHMec whereas cathepsin H did not. The substrate and inhibitor specificities of cathepsin H suggest that unlike cathepsins B, L, and S, it cannot accommodate peptides with aromatic side chains in P2. Cathepsins L and S can accommodate the aromatic side chain of tyrosine in P1 readily, whereas cathepsins H and B cannot. The specificities of each enzyme for synthetic substrates and inhibitors have enabled the construction of models of the architecture of the active sites of the mammalian cysteine proteinases which clearly show the differences between the four enzymes. A significant characteristic of cathepsin S is that it can hydrolyze insoluble elastin at both acidic and neutral pH; this distinguishes it from all of the other lysosomal proteinases.
authors.affiliation: Department of Biochemistry, Virginia Polytechnic Institute and State University, Blacksburg 24061.
authors.forename: X Q
authors.initials: XQ
authors.lastname: Xin
authors.forename: B
authors.initials: B
authors.lastname: Gunesekera
authors.forename: R W
authors.initials: RW
authors.lastname: Mason
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D056655:Cathepsin H; D002403:Cathepsins / Q000302:isolation & purification / Q000378:metabolism*; D002417:Cattle; D003546:Cysteine Endopeptidases; D004549:Elastin / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry / Q000378:metabolism; D013154:Spleen / Q000201:enzymology; D013379:Substrate Specificity
chemical_list: D010455:Peptides; D004549:Elastin; D002403:Cathepsins; D003546:Cysteine Endopeptidases; D056655:Cathepsin H; C028217:cathepsin S