[GTP-dependent dopamine reception in central nervous system tissues in the pond snail].
Guanosine-5'-triphosphate (GTP)-dependent binding of the agonist D1 dopamine (DA) receptor ligand [H3]-SKF 38393 is described. It is shown that binding of [H3]-SKF 38393 with two different populations takes place in the presence of guanylyl nucleotides, when they are absent--with one population. It is demonstrated using GDP-alpha-P33 binding analysis that the GDP in equilibrium with GTP exchange rate gets higher as a result of activation of DA receptors in the membrane from the mollusc nervous tissues. Influence of the catalytic subunit of the protein kinase A (cPKA) on the [H3]-SKF 38393 and GDP-alpha-P33 is investigated. Obvious influence of the cPKA on [H3]-SKF 38393 binding is not observed, while basal and DA-induced GDP in equilibrium with GTP exchange in membranes of the mollusc nervous tissues is considerably inhibited.
Prudnikov
I M
IM
Tsyvkin
V N
VN
Kastrikina
T F
TF
D000818:Animals; D002490:Central Nervous System / Q000502:physiology*; D006153:Guanosine Diphosphate / Q000378:metabolism; D006160:Guanosine Triphosphate / Q000378:metabolism / Q000502:physiology*; D008195:Lymnaea / Q000502:physiology*; D008566:Membranes / Q000378:metabolism; D010766:Phosphorylation; D011869:Radioligand Assay; D017447:Receptors, Dopamine D1 / Q000502:physiology*; D017448:Receptors, Dopamine D2 / Q000502:physiology*
D017447:Receptors, Dopamine D1; D017448:Receptors, Dopamine D2; D006153:Guanosine Diphosphate; D006160:Guanosine Triphosphate
GTF-zavisimaia retseptsiia dofamina v tkaniiakh tsentral'noĭ nervnoĭ sistemy bol'shogo prudovika.