title: On-line coupling of in vivo microdialysis with tandem mass spectrometry.
abstract: The capability of interfacing in vivo microdialysis with mass spectrometry has been demonstrated. The goal of this research was to demonstrate the feasibility of real-time analysis in biological systems using microdialysis in combination with tandem mass spectrometry (MS/MS). Microdialysis sampling was accomplished by surgically implanting a small microdialysis probe into a tissue or area of interest. Molecules diffuse through the membrane of the microdialysis probe due to concentration differences. These molecules are collected in a sample loop and analyzed by tandem mass spectrometry. Sequential injections can be made in as little as 2 min. This capability is advantageous in the study of molecules with very rapid elimination rates. Tris(2-chloroethyl)phosphate (TRCP) was used as a model compound in the development of this analytical technique. As an example of an application of the microdialysis/MS/MS technique, plasma concentration vs time curves were obtained and compared with the plasma concentration profiles obtained using conventional studies. For the microdialysis/MS/MS studies, the average slope from three animals was -0.086 min-1. In comparison, the average slope from four animals from the conventional studies was -0.035 min-1.
authors.affiliation: Laboratory of Molecular Biophysics, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709.
authors.forename: L J
authors.initials: LJ
authors.lastname: Deterding
authors.forename: K
authors.initials: K
authors.lastname: Dix
authors.forename: L T
authors.initials: LT
authors.lastname: Burka
authors.forename: K B
authors.initials: KB
authors.lastname: Tomer
mesh_terms: D000818:Animals; D001798:Blood Proteins / Q000032:analysis; D002853:Chromatography, Liquid; D003956:Dialysis / Q000379:methods*; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D051381:Rats; D011916:Rats, Inbred F344
chemical_list: D001798:Blood Proteins