Changes in the glutathione redox system during ischemia and reperfusion in rat liver.
After 60 min of reperfusion following 60 min of ischemia, the ischemia-induced decrease in liver tissue adenosine triphosphate (ATP) concentration had recovered by 66%, and full recovery of mitochondrial function--that is, the respiratory control index (RCI) and the rate of oxygen consumption in state-III respiration (ST III O2)--was observed. In contrast, liver tissue ATP concentration had recovered by only 13%, and marked low RCI and ST III O2 were observed after 60 min of reperfusion following 180 min of ischemia. Intermediate results were observed in rats after 60 min of reperfusion following 120 min of ischemia. Liver tissue hypoxanthine and xanthine, substrates of xanthine oxidase, increased ischemic time dependently. Liver tissue concentrations of the reduced form of glutathione (GSH) and the oxidized form of glutathione (GSSG) and activities of glutathione peroxidase and glutathione reductase did not change after 60 min of reperfusion following 60 min of ischemia. In contrast, GSH concentration and glutathione peroxidase activity decreased significantly after 60 min of reperfusion following 180 min of ischemia. Since the glutathione redox system is an important contributor to the scavenging of free radicals after reperfusion following a long time of ischemia, the free radical scavenging ability might decrease in spite of enhancement of free radical generation, which might play an important role in the inhibition of the recovery of tissue ATP concentrations and mitochondrial function.
Kobayashi
H
H
Dept. of Surgery II, Faculty of Medicine, University of Nagoya, Japan.
Nonami
T
T
Kurokawa
T
T
Kitahara
S
S
Harada
A
A
Nakao
A
A
Sugiyama
S
S
Ozawa
T
T
Takagi
H
H
D000255:Adenosine Triphosphate / Q000032:analysis; D000818:Animals; D005979:Glutathione Peroxidase / Q000032:analysis*; D005980:Glutathione Reductase / Q000032:analysis*; D007042:Hypoxanthines / Q000032:analysis; D007511:Ischemia / Q000201:enzymology*; D008099:Liver / Q000098:blood supply* / Q000201:enzymology*; D008297:Male; D008930:Mitochondria, Liver / Q000201:enzymology / Q000378:metabolism; D010084:Oxidation-Reduction; D051381:Rats; D017208:Rats, Wistar; D015424:Reperfusion; D015427:Reperfusion Injury / Q000209:etiology; D014970:Xanthines / Q000032:analysis
D007042:Hypoxanthines; D014970:Xanthines; D000255:Adenosine Triphosphate; D005979:Glutathione Peroxidase; D005980:Glutathione Reductase