A biological assay for the detection of Myrothecium spp. produced macrocyclic trichothecenes.
A rapid, inexpensive bioassay to detect Myrothecium spp.-produced macrocyclic trichothecenes was developed. Media containing Myrothecium isolates were inoculated with Chlorella vulgaris, Ustilago maydis and Trichoderma viride. Based on width of the inhibition zone, isolates could be classified as highly toxigenic, non-toxigenic and intermediate. Whereas, C. vulgaris and U. maydis showed significant differences in their response to toxigenic and non-toxigenic isolates, T. viride did not. Production of roridins and verrucarins by the toxigenic isolates (by bioassay) was confirmed by thin layer chromatography and high performance liquid chromatography. This bioassay system, combined with confirmation chemical analyses, increases our ability to detect toxigenic fungal isolates.
Bean
G A
GA
Department of Botany, University of Maryland, College Park 20742.
Jarvis
B B
BB
Aboul-Nasr
M B
MB
D001681:Biological Assay; D002708:Chlorella / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D003904:Mitosporic Fungi / Q000378:metabolism*; D014242:Trichoderma / Q000187:drug effects; D014255:Trichothecenes / Q000032:analysis* / Q000096:biosynthesis; D014588:Ustilago / Q000187:drug effects
D014255:Trichothecenes