title: Comparison of the modelled thyroxine binding site in TBG with the experimentally determined site in transthyretin.
abstract: The structure of cleaved thyroxine-binding globulin (TBG) has been modelled on the crystal structure of cleaved alpha 1-antitrypsin (a member of the serine proteinase inhibitor, serpin, superfamily) based on the high sequence homology exhibited by the two proteins. Particular attention was paid to the identification and modelled characteristics of the thyroxine binding site. The primary aim of the study was to compare the site qualitatively with the crystallographically determined binding site of transthyretin, the other major transporter of thyroxine, in an attempt to explain the higher binding affinity of the site compared with the known thyroxine binding site in transthyretin (10(10) versus 10(8) M-1). The proposed binding site shares some similar characteristics with the transthyretin binding site but also includes a cluster of aromatic residues which are entirely absent in transthyretin. It is proposed that this might account for the substantial difference in binding affinities.
authors.affiliation: Laboratory of Molecular Biophysics, Oxford, UK.
authors.forename: C J
authors.initials: CJ
authors.lastname: Terry
authors.forename: C C
authors.initials: CC
authors.lastname: Blake
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D006801:Humans; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011228:Prealbumin / Q000737:chemistry* / Q000378:metabolism; D011485:Protein Binding; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D016415:Sequence Alignment; D015843:Serpins / Q000737:chemistry; D013974:Thyroxine / Q000378:metabolism*; D013975:Thyroxine-Binding Proteins / Q000737:chemistry* / Q000378:metabolism; D000515:alpha 1-Antitrypsin / Q000737:chemistry
chemical_list: D011228:Prealbumin; D015843:Serpins; D013975:Thyroxine-Binding Proteins; D000515:alpha 1-Antitrypsin; D013974:Thyroxine