Small nuclear ribonucleoprotein polypeptides in rat sperm.
1. Immunoblot analysis of rat sperm head proteins revealed the presence of polypeptides recognized by anti-Sm serum obtained from patients with systemic lupus erythematosus. 2. Two of these polypeptides have molecular weights of 26,000 and 15,000 and they were identified as small nuclear ribonucleoprotein components present in other rat tissues. 3. When the autoimmune serum was used in the immuno-gold procedure for electron microscopy, gold particles were found only on the sperm nucleus. 4. The results indicate that some polypeptides of the small nuclear ribonucleoprotein complex are components of the rat sperm chromatin.
Brito
M
M
Instituto de Bioquimica, Facultad de Ciencias, Universidad Austral de Chile, Valdivia.
Concha
I I
II
Pessot
C A
CA
Mezzano
S
S
Burzio
L O
LO
D000818:Animals; D002843:Chromatin / Q000737:chemistry; D015151:Immunoblotting; D007150:Immunohistochemistry; D008180:Lupus Erythematosus, Systemic / Q000276:immunology; D008297:Male; D008854:Microscopy, Electron; D008970:Molecular Weight; D051381:Rats; D017411:Ribonucleoproteins, Small Nuclear / Q000032:analysis* / Q000737:chemistry; D013094:Spermatozoa / Q000737:chemistry* / Q000648:ultrastructure
D002843:Chromatin; D017411:Ribonucleoproteins, Small Nuclear