title: Study of kidney and liver viability in the rat after exclusive aortic perfusion using intracellular ATP measurement.
abstract: To find whether the liver can be procured after exclusive aortic perfusion, three organ perfusion models were used in three groups of donor rats. Group 1 underwent liver wash-out via the portal vein; in group 2, the kidneys alone were perfused via the aorta; and group 3 underwent simultaneous aortic perfusion of liver and kidneys. All perfusion flow rates in the three groups were adjusted to physiological values. Harvested organs were transplanted and recipient animals were killed 4 h after transplantation to study liver and kidney viability by using intracellular ATP measurement. Liver ATP was lower (P < 0.005) in the portal perfusion group (group 1: 1.396 +/- 0.412) than in the aortic perfusion group (group 3: 2.181 +/- 0.061). Kidney ATP was comparable in groups 2 and 3:1.066 +/- 0.09 vs 1.059 +/- 0.273 (mumol/g) tissue). Liver cooling was quicker with portal perfusion than with the aortic flush (20 degrees C in 20 s vs 15 degrees C in 60 s). Aortic perfusion at a physiologic flow rate has no detrimental effect on renal viability studied by intracellular ATP measurement. We conclude that liver cooling via the aortic route only is a good alternative to portal perfusion and seems to give good preservation. Application of this observation to emergency procurement in humans is still the subject of controversy.
authors.affiliation: Laboratory of Experimental Surgery, University of Paris, Bicêtre Hospital, France.
authors.forename: H
authors.initials: H
authors.lastname: Bittard
authors.forename: L
authors.initials: L
authors.lastname: Chiche
authors.forename: M
authors.initials: M
authors.lastname: Moukarzel
authors.forename: D
authors.initials: D
authors.lastname: Douguet
authors.forename: G
authors.initials: G
authors.lastname: Benoit
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001011:Aorta; D005069:Evaluation Studies as Topic; D006085:Graft Survival; D007668:Kidney / Q000378:metabolism; D016030:Kidney Transplantation; D008099:Liver / Q000378:metabolism; D016031:Liver Transplantation; D009926:Organ Preservation / Q000379:methods*; D010477:Perfusion; D051381:Rats; D011914:Rats, Inbred BN; D009927:Tissue and Organ Procurement
chemical_list: D000255:Adenosine Triphosphate