Identification of jun-B as third member in human antioxidant response element-nuclear proteins complex.
Deletion mutagenesis in human NAD(P)H:Quinone Oxidoreductase (NQO1) gene and transfection studies into mammalian cells identified a segment of DNA designated as human Antioxidant Response Element (hARE) responsible for high basal expression in tumor cells and its induction by beta-naphthoflavone (beta-NF). The twenty four base pairs of the hARE contains an essential cis-element AP1 binding site and has been shown to bind to jun-D and c-fos proteins from mouse hepatoma (Hepa-1) nuclear extract. In the present report, we have identified jun-B as the third major protein in the hARE-Hepa-1 proteins complex observed in the band shift assays.
Li
Y
Y
Department of Pharmacology, Fox Chase Cancer Center, Philadelphia, PA 19111.
Jaiswal
A K
AK
D001483:Base Sequence; D002467:Cell Nucleus / Q000737:chemistry*; D006801:Humans; D008969:Molecular Sequence Data; D016660:NAD(P)H Dehydrogenase (Quinone) / Q000235:genetics*; D016755:Proto-Oncogene Proteins c-jun / Q000235:genetics / Q000302:isolation & purification*; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics*; D014158:Transcription, Genetic
D016755:Proto-Oncogene Proteins c-jun; D016660:NAD(P)H Dehydrogenase (Quinone)