title: The effect of dexamethasone and endotoxin administration on biliary IgA and bacterial adherence.
abstract: Adherence of bacteria to the intestinal epithelial cell may be the crucial initiating event for invasion and translocation and is normally prevented by both immune (IgA) and nonimmune (mucus, peristalsis, desquamation) mucosal defense mechanisms. The purpose of the present study was to examine the effect of endotoxin administration on mucosal immunity and to define the role of glucocorticoids, commonly released during endotoxicosis, in this process. Thirty female Fisher rats were randomly assigned to three groups of 10 animals each. Group I (CONT), was fed rat chow and H2O ad lib., Group II (DEX) was administered 0.8 mg/kg subcutaneously of dexamethasone, and Group III (ETX) was given 1 mg/kg of endotoxin. Twenty-four hours later animals were sacrificed and mesenteric lymph nodes and vigorously washed stool-free ceca were collected and cultured. Bile was collected and assayed for IgA from 5 animals in each group. A significant decrease (P < 0.05) in secretory IgA was noted in animals treated with either dexamethasone or endotoxin (CONT = 332 +/- 42, DEX = 78 +/- 24, ETX = 68 +/- 16 micrograms/mg protein +/- SEM). No difference in S-IgA between animals in the dexamethasone-treated group and the endotoxin-treated group was noted (P = NS). A statistically significant increase (P < 0.001) in bacteria adherent to the cecal wall in both the dexamethasone-treated rats and the endotoxin-treated rats over that in = 7.5 +/- 0.8, CONT = 6.4 +/- 0.6 cfu/g(log10) +/- SD). Our results suggest that endotoxin or glucocorticoid administration results in significant bacterial adherence to the cecal mucosa and a decrease in IgA.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Surgery, Humana Hospital-Michael Reese Medical Center, University of Illinois, Chicago 60616.
authors.forename: J C
authors.initials: JC
authors.lastname: Alverdy
authors.forename: E
authors.initials: E
authors.lastname: Aoys
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion / Q000187:drug effects*; D001646:Bile / Q000187:drug effects / Q000276:immunology*; D002432:Cecum / Q000187:drug effects / Q000382:microbiology; D015169:Colony Count, Microbial; D004731:Endotoxins / Q000494:pharmacology*; D004926:Escherichia coli / Q000502:physiology; D005260:Female; D007071:Immunoglobulin A, Secretory / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000187:drug effects / Q000276:immunology*; D008198:Lymph Nodes / Q000187:drug effects / Q000382:microbiology; D008643:Mesentery; D011513:Proteus mirabilis / Q000502:physiology; D051381:Rats
chemical_list: D004731:Endotoxins; D007071:Immunoglobulin A, Secretory