[The construction of rich genomic library of bovine Y chromosome].
According to the fundamentals of DNA renatured kinetics, we made a study in which genomic DNA from male cattle was completely digested with Sau 3A, while female DNA was sheared to an average size of 500 bp fragments. The Y derived DNA sequence rich library of bovine has been contracted with pUC19 plasmid as vector and JM109 as host bacteria by reassociation of male and female DNA. Male and female DNA probes, were used. The result of colony hybridization showed that out of 1000 recombinants analysed, 6 were found to be stronger signal with male probe. Analysis of Pvu' I digested DNA fragment revealed that most of the inserts were in the 90-300 bp.
Li
M
M
Institute of Developmental Biology, Academia, Sinica.
Sun
Z
Z
Shi
Y
Y
D000818:Animals; D002417:Cattle; D003001:Cloning, Molecular; D015342:DNA Probes; D005260:Female; D005818:Genetic Engineering; D015698:Genomic Library; D008297:Male; D010957:Plasmids / Q000235:genetics; D014998:Y Chromosome
D015342:DNA Probes