[The effect of verapamil and nifedipine on cholesterol esterification and accumulation in macrophages].
Administration of verapamil and nifedipine in doses of 10-75 microM into the culture of mouse peritoneal macrophages incubated in a non-lipid medium with acetylated low density lipoproteins (50 g protein/ml) decreased the incorporation of 14C oleate into cholesterol esters by 50-90%, increased the content of intracellular free cholesterol by 19-79% and did not influence the content of total cholesterol in macrophages. Administration of verapamil and nifedipine in doses of 25 and 50 microM in the culture of macrophages previously enriched with cholesterol reduced the rate of cholesterol esterification by 22-62%. After combined administration of verapamil and nifedipine in doses of 25 microM into the medium the rate of cholesterol esterification in macrophages fell by 72.4%.
Dushkin
M I
MI
Ivanova
M V
MV
D000818:Animals; D001202:Ascitic Fluid / Q000166:cytology; D002478:Cells, Cultured / Q000187:drug effects / Q000378:metabolism; D002784:Cholesterol / Q000378:metabolism*; D002788:Cholesterol Esters / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D006801:Humans; D008077:Lipoproteins, LDL / Q000187:drug effects / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D009543:Nifedipine / Q000494:pharmacology*; D014700:Verapamil / Q000494:pharmacology*
D002788:Cholesterol Esters; D008077:Lipoproteins, LDL; D002784:Cholesterol; D014700:Verapamil; D009543:Nifedipine
Vliianie verapamila i nifedipina na ésterifikatsiiu i nakoplenie kholesterina v makrofagakh.