The length of the interdomain region of the L7/L12 protein is important for its function.
Several mutated L7/L12 proteins with changed interdomain regions were obtained. The results showed that the flexible region comprising the 39-52 amino acid residues is functionally important. Its length, but not its amino acid composition, is crucial for the function.
Bubunenko
M G
MG
Institute of Protein Research, Russian Academy of Sciences, Pushchino, Moscow Region.
Chuikov
S V
SV
Gudkov
A T
AT
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics; D008969:Molecular Sequence Data; D016296:Mutagenesis; D010441:Peptide Chain Elongation, Translational; D011489:Protein Denaturation; D012269:Ribosomal Proteins / Q000737:chemistry / Q000378:metabolism*; D013329:Structure-Activity Relationship; D013816:Thermodynamics
D001426:Bacterial Proteins; D012269:Ribosomal Proteins; C016700:ribosomal protein L7-L12