title: Eggshell precursor proteins of Fasciola hepatica, I. Structure and expression of vitelline protein B.
abstract: Antibody raised against the major eggshell protein of Fasciola hepatica (vitelline protein B, vpB) is employed to isolate cDNAs from an expression library and to localize the protein in whole worms. Two cDNAs corresponding to the protein are homologous through the N-terminal and C-terminal coding regions and widely divergent internally. No repeated regions are apparent and no significant sequence homology is seen with chorion proteins of other genera although the amino acid composition closely reflects that of other chorion proteins. Microheterogeneity observed in the vpB is due to the presence of multiple coding sequences, transcripts and a gradient of post-translational modification. Relative transcription of the vpB mRNA throughout the female reproductive tract is demonstrated.
authors.affiliation: Department of Medical Biochemistry and Genetics, Texas A&M University College Station 77843-1114.
authors.forename: A C
authors.initials: AC
authors.lastname: Rice-Ficht
authors.forename: K A
authors.initials: KA
authors.lastname: Dusek
authors.forename: G J
authors.initials: GJ
authors.lastname: Kochevar
authors.forename: J H
authors.initials: JH
authors.lastname: Waite
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000235:genetics; D004527:Egg Proteins / Q000737:chemistry / Q000235:genetics*; D005210:Fasciola hepatica / Q000235:genetics*; D017238:Genes, Helminth / Q000235:genetics*; D015801:Helminth Proteins / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D010063:Ovum / Q000737:chemistry; D017385:Sequence Homology; D014817:Vitelline Membrane / Q000737:chemistry*
chemical_list: D004527:Egg Proteins; D015801:Helminth Proteins; C077550:vitelline protein B1, Fasciola hepatica; C077551:vitelline protein B2, Fasciola hepatica; D004247:DNA