title: Surgical glove powders bind latex antigens.
abstract: Latex surgical gloves have recently been identified as a potential source of allergens. Much of the current information suggests that the soluble proteins in latex may cause significant reactions in sensitive individuals. The starch powders used as a lubricant on some latex gloves have also been identified as potential allergens in some patients. In this study, we determined these powders to act as potential carriers of latex allergens. We have produced a polyclonal antiserum to be used as a reagent to study latex proteins. By Western blot analysis, we identified a significant interaction between latex proteins and starch powders. The binding of latex proteins to starch particles results in a glove particle that may have an increased potential to act as an allergen. The latex protein-starch particles represent a potential mechanism for exposure and sensitization of health care workers to latex allergens. Elimination of these particles from the operating room should reduce the route of sensitization and the potential for adverse reactions to latex.
authors.affiliation: Laboratory of Macrophage Biology, Guthrie Foundation for Medical Research, Sayre, Pa. 18840.
authors.forename: D
authors.initials: D
authors.lastname: Beezhold
authors.forename: W C
authors.initials: WC
authors.lastname: Beck
mesh_terms: D000327:Adsorption; D000818:Animals; D000941:Antigens / Q000276:immunology* / Q000378:metabolism; D015153:Blotting, Western; D004195:Disease Models, Animal; D004342:Drug Hypersensitivity / Q000097:blood / Q000209:etiology / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005069:Evaluation Studies as Topic; D005932:Gloves, Surgical / Q000592:standards*; D006801:Humans; D007106:Immune Sera; D007840:Latex / Q000009:adverse effects* / Q000378:metabolism; D011208:Powders; D011485:Protein Binding; D011817:Rabbits; D013213:Starch / Q000009:adverse effects* / Q000276:immunology / Q000378:metabolism
chemical_list: D000941:Antigens; D007106:Immune Sera; D007840:Latex; D011208:Powders; D013213:Starch