title: Factors affecting the rate of contact activation in human plasma.
abstract: The rate of high molecular weight kininogen cleavage in human plasma treated with dextran sulfate depends largely on the length of preincubation at 37 degrees C and the sample size during preincubation. The longer the preincubation with aliquots < 100 microliters, the more refractory to contact activation the plasma became. This effect is not due to prekallikrein or factor XII consumption/inhibition since both proteins are functional in clotting assays and detectable in immunoblots even after prolonged incubation times at 37 degrees C.
authors.affiliation: Department of Medicine, State University of New York, Stony Brook 11794-8161.
authors.forename: T
authors.initials: T
authors.lastname: Brunnée
authors.forename: G
authors.initials: G
authors.lastname: Coy
authors.forename: S R
authors.initials: SR
authors.lastname: Reddigari
authors.forename: M
authors.initials: M
authors.lastname: Silverberg
mesh_terms: D001777:Blood Coagulation; D004789:Enzyme Activation; D005174:Factor XII / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D007704:Kininogens / Q000097:blood* / Q000302:isolation & purification; D011288:Prekallikrein / Q000378:metabolism
chemical_list: D007704:Kininogens; D005174:Factor XII; D011288:Prekallikrein