The anticodon triplet is not sufficient to confer methionine acceptance to a transfer RNA.
Previous work suggested that the presence of the anticodon CAU alone was enough to confer methionine acceptance to a tRNA. Conversions of Escherichia coli nonmethionine tRNAs to a methionine-accepting species were obtained by substitutions reconstructing the whole methionine anticodon loop together with preservation (or introduction) of the acceptor stem base A73. We show here that the CAU triplet alone is unable to confer methionine acceptance when transplanted into a yeast aspartic tRNA. Both non-anticodon bases of the anticodon loop of yeast tRNA(Met) and A73 are required in addition to CAU for methionine acceptance. The importance of these non-anticodon bases in other CAU-containing tRNA frameworks was also established. These specific non-anticodon base interactions make a substantial thermodynamic contribution to the methionine acceptance of a transfer RNA.
Senger
B
B
Laboratoire de Biochimie, Centre National de la Recherche Scientifique, Strasbourg, France.
Despons
L
L
Walter
P
P
Fasiolo
F
F
D000926:Anticodon / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D007700:Kinetics; D008715:Methionine / Q000378:metabolism*; D008961:Models, Structural; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012358:RNA, Transfer, Met / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D014158:Transcription, Genetic
D000926:Anticodon; D012358:RNA, Transfer, Met; D008715:Methionine