title: Differentially regulated malate synthase genes participate in carbon and nitrogen metabolism of S. cerevisiae.
abstract: We have isolated a second gene (MLS1), which in addition to DAL7, encodes malate synthase from S. cerevisiae. Expression of the two genes is specific for their physiological roles in carbon and nitrogen metabolism. Expression of MLS1, which participates in the utilization of non-fermentable carbon sources, is sensitive to carbon catabolite repression, but nearly insensitive to nitrogen catabolite repression. DAL7, which participates in catabolism of the nitrogenous compound allantoin, is insensitive to carbon catabolite repression, but highly sensitive to nitrogen catabolite repression. Results obtained with null mutations in these genes suggest that S. cerevisiae contains at least one and perhaps two additional malate synthase genes.
authors.affiliation: Institut fuer Allgemeine Biochemie der Universitaet, Wien, Austria.
authors.forename: A
authors.initials: A
authors.lastname: Hartig
authors.forename: M M
authors.initials: MM
authors.lastname: Simon
authors.forename: T
authors.initials: T
authors.lastname: Schuster
authors.forename: J R
authors.initials: JR
authors.lastname: Daugherty
authors.forename: H S
authors.initials: HS
authors.lastname: Yoo
authors.forename: T G
authors.initials: TG
authors.lastname: Cooper
mesh_terms: D019760:Amidine-Lyases; D000595:Amino Acid Sequence; D001483:Base Sequence; D002244:Carbon / Q000378:metabolism*; D004271:DNA, Fungal; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D007700:Kinetics; D008190:Lyases / Q000378:metabolism; D008292:Malate Synthase / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D016296:Mutagenesis; D009584:Nitrogen / Q000378:metabolism*; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid
chemical_list: D004271:DNA, Fungal; D002244:Carbon; D008292:Malate Synthase; D008190:Lyases; D019760:Amidine-Lyases; C033757:ureidoglycollate lyase; D009584:Nitrogen