Naturally occurring SLE anti-DNA antibodies recognize unique conformation on DNA-lysine photoadduct.
Native calf thymus DNA has been covalently modified with lysine under UV-A light. Human autoantibodies on purification through affinity column of native DNA linked to polylysyl-Sepharose 4B showed almost equal recognition of DNA and photoadduct. The recognition of DNA-lysine photoadduct by the affinity-purified autoantibodies might be helpful in understanding their origin in SLE vis-à-vis the role of positively charged amino acids in the pathogenesis of autoimmune diseases.
Alam
K
K
Department of Biochemistry, Faculty of Medicine, A.M.U. Aligarh, India.
Islam
N
N
Hasan
R
R
Ali
A
A
Ali
R
R
D000818:Animals; D000974:Antibodies, Antinuclear / Q000302:isolation & purification; D001665:Binding Sites; D001667:Binding, Competitive; D002417:Cattle; D004247:DNA / Q000737:chemistry / Q000276:immunology*; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000276:immunology*; D008239:Lysine / Q000737:chemistry; D009690:Nucleic Acid Conformation; D010777:Photochemistry
D000974:Antibodies, Antinuclear; D004247:DNA; D008239:Lysine