Chemical contrast in X-ray microscopy and spatially resolved XANES spectroscopy of organic specimens.
The scanning transmission x-ray microscope at the National Synchrotron Light Source has been used to record x-ray absorption near-edge structure (XANES) spectra from 0.01-square-micrometer regions of organic specimens. The spectral features observed reflect the molecular structure of the dominant absorbing atoms and provide the contrast mechanism for high-resolution imaging with chemical sensitivity. This technique was used with x-ray energies near the carbon K absorption edge to identify and map separate phases in various polymer blends and to map the DNA distribution in chromosomes with a spatial resolution of 55 nanometers.
Ade
H
H
Department of Physics, State University of New York, Stony Brook, NY 11794.
Zhang
X
X
Cameron
S
S
Costello
C
C
Kirz
J
J
Williams
S
S
D000180:Acrylic Resins / Q000737:chemistry; D002875:Chromosomes / Q000737:chemistry; D004247:DNA / Q000032:analysis / Q000737:chemistry; D007887:Fabaceae / Q000235:genetics; D008853:Microscopy / Q000379:methods*; D010946:Plants, Medicinal; D011126:Polypropylenes / Q000737:chemistry; D011137:Polystyrenes / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000032:analysis / Q000737:chemistry; D013057:Spectrum Analysis / Q000379:methods*; D014965:X-Rays
D000180:Acrylic Resins; D011126:Polypropylenes; D011137:Polystyrenes; C010504:polyacrylonitrile; D012710:Serum Albumin, Bovine; D004247:DNA