Effect of aldose reductase inhibitors on lenticular dulcitol level in galactose fed rats.
We have shown that galactose cataract development is delayed or inhibited with the administration of aldose reductase inhibitors (ARIs). Dulcitol forms and accumulates in the lens of rats fed galactose. We undertook investigations to study the effectiveness of ARIs in preventing the formation and accumulation of dulcitol in the lens. Young Sprague Dawley rats were fed Purina Rat Chow with 50% galactose either with or without 15 mg sorbinil, 0.15, 0.5, or 1.0 mg E-0722/day/Kg body weight. At desired intervals following the initiation of diets, the lenses were processed for the determination of galactose and dulcitol levels. The lenticular dulcitol increased significantly in all animals fed galactose reaching a maximum level by approximately 15 days with comparatively lower levels in the groups fed ARIs with galactose; this increase was dose dependent in the groups fed E-0722. There was a subsequent, rapid drop in lenticular dulcitol by 18 days in all dietary groups. Interestingly, a second peak of increased lenticular dulcitol was observed in all groups. The correlation between dulcitol accumulation and cataract development is discussed.
Unakar
N J
NJ
Department of Biological Sciences, Oakland University, Rochester, Michigan.
Tsui
J Y
JY
Johnson
M J
MJ
D000449:Aldehyde Reductase / Q000037:antagonists & inhibitors*; D000818:Animals; D002386:Cataract / Q000139:chemically induced / Q000517:prevention & control; D004040:Dietary Carbohydrates; D004376:Galactitol / Q000378:metabolism*; D005690:Galactose; D007093:Imidazoles / Q000494:pharmacology*; D048289:Imidazolidines; D007908:Lens, Crystalline / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
D004040:Dietary Carbohydrates; D007093:Imidazoles; D048289:Imidazolidines; D004376:Galactitol; C037070:M 79175; D000449:Aldehyde Reductase; C026411:sorbinil; D005690:Galactose