[A combined effect of cortisol and concanavalin A on calcium ion contents in thymic lymphocytes].
The influence of hydrocortisone (1-10 microM) on mitogen-induced Ca level was studied in cytoplasma thymocytes of rat. The investigation was conducted with the use of fluorescence technique (FURA-2AM). A specific Ca-blocking effect of the hormone was observed in the thymocytes of intact animals. Hydrocortisone did not change Ca-response of mitogen-stimulated hormone-resistant thymic lymphocytes. A conclusion was made about applicability of this method to assess the response of the cells to glucocorticoids in vitro.
Sergeev
P V
PV
Dukhanin
A S
AS
Bulaev
N N
NN
D000818:Animals; D002118:Calcium / Q000097:blood*; D003208:Concanavalin A / Q000494:pharmacology*; D003593:Cytoplasm / Q000737:chemistry / Q000187:drug effects; D006854:Hydrocortisone / Q000494:pharmacology*; D066298:In Vitro Techniques; D008214:Lymphocytes / Q000737:chemistry / Q000187:drug effects*; D051381:Rats; D013950:Thymus Gland / Q000737:chemistry / Q000187:drug effects*
D003208:Concanavalin A; D002118:Calcium; D006854:Hydrocortisone
Sovmestnoe deĭstvie kortizola i konkanavalina A na soderzhanie ionov kal'tsiia v limfotsitakh timusa.