Some biological properties of a rhabdovirus isolated from penaeid shrimps.
Some of the relevant biological properties of a rhabdovirus isolated from penaeid shrimps (RPS) were examined. The virus replicated in an established fish cell line, epithelioma papulosum cyprini (EPC) which allowed for the development of a quantitative plaque assay protocol. Virus replication was not inhibited by the DNA antagonist, 5-bromo-2'-deoxyuridine (20 micrograms/ml). Virus infectivity was sensitive to 20% ethyl ether, low pH, and to 37 degrees C. The virus showed marked lability to repeated freezing and thawing and storage at -10 degrees C, but was stable at -70 degrees C for several weeks. The virus particle to infectious unit ratio in EPC was found to be 30.
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Department of Microbiology, University of Hawaii, Honolulu.
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D000818:Animals; D002478:Cells, Cultured; D003588:Cytopathogenic Effect, Viral; D033364:Decapoda / Q000382:microbiology*; D066298:In Vitro Techniques; D012209:Rhabdoviridae / Q000254:growth & development / Q000302:isolation & purification*; D013045:Species Specificity; D010948:Viral Plaque Assay; D014779:Virus Replication