[Cryopreservation of spermatozoa of a transgenic mouse].
Spermatozoa of a homozygous transgenic mouse, in which the firefly luciferase gene was expressed under the control of beta-actin promoter, were frozen at -196 degrees C. One fourth of the frozen sperm was later thawed and used for in vitro fertilization. Thirty-six of 65 oocytes (55.4%) developed to the 2-cell stage. All the 2-cell embryos were transferred to the oviducts of pseudopregnant recipients and 23 young (63.9%, 23/36) were born. All of young analyzed carried the transgene and showed the luciferase gene expression.
Nakagata
N
N
NT Science Co., Ltd., Tokyo, Japan.
Matsumoto
K
K
Anzai
M
M
Takahashi
A
A
Takahashi
Y
Y
Matsuzaki
Y
Y
Miyata
K
K
D000199:Actins; D000818:Animals; D015925:Cryopreservation / Q000662:veterinary*; D004624:Embryo Transfer; D005307:Fertilization in Vitro; D015971:Gene Expression Regulation, Enzymologic; D008156:Luciferases / Q000235:genetics*; D008297:Male; D051379:Mice; D008822:Mice, Transgenic / Q000235:genetics*; D012098:Reproduction; D013094:Spermatozoa
D000199:Actins; D008156:Luciferases