Calyculin A induces contractile ring-like apparatus formation and condensation of chromosomes in unfertilized sea urchin eggs.
Calyculin A, a protein phosphatase inhibitor, induced cleavage-like morphological change in unfertilized sea urchin eggs. A contractile ring-like apparatus containing both filamentous actin and myosin was formed in the cleavage furrow. Wheat germ agglutinin receptors were also found in the same region. The eggs did not develop further after constriction of the ring. No aster-like microtubular structure was found in the calyculin A-treated eggs. The cleavage was not inhibited by the antimicrotubule drug griseofulvin. Calyculin A also increased histone H1 kinase activity and induced chromosome condensation. These changes also occurred in the presence of emetine (an inhibitor of protein synthesis) and aphidicolin (an inhibitor of DNA synthesis). It is suggested that calyculin A induced these changes in the sea urchin eggs by inhibiting the activity of protein phosphatase 1.
Tosuji
H
H
Department of Biology, Faculty of Science, Toho University, Funabashi, Japan.
Mabuchi
I
I
Fusetani
N
N
Nakazawa
T
T
D008841:Actin Cytoskeleton / Q000187:drug effects / Q000502:physiology / Q000648:ultrastructure; D000199:Actins / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D002273:Carcinogens / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D002875:Chromosomes / Q000187:drug effects* / Q000502:physiology; D005260:Female; D005306:Fertilization; D006657:Histones / Q000302:isolation & purification / Q000378:metabolism; D008387:Marine Toxins; D009132:Muscles / Q000378:metabolism; D009218:Myosins / Q000302:isolation & purification / Q000378:metabolism; D010063:Ovum / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D010080:Oxazoles / Q000494:pharmacology*; D010766:Phosphorylation; D011817:Rabbits; D012617:Sea Urchins / Q000502:physiology*; D013045:Species Specificity
D000199:Actins; D002273:Carcinogens; D006657:Histones; D008387:Marine Toxins; D010080:Oxazoles; C059041:calyculin A; D009218:Myosins