Detection of glucocorticoid-receptor complex oligomers in nuclear extracts from cells exposed to hormone at physiological temperature.
When HeLa cells were incubated with tritiated dexamethasone mesylate at 2 degrees C, sodium dodecyl sulfate-polyacrylamide gel electrophoresis of cytosolic and nuclear extracts revealed the presence of two monomeric receptor complex forms with estimated molecular masses of about 98 and 87 kDa. If cells were subjected to crosslinking with glutaraldehyde, a third form consisting of a 250 kDa oligomer was also detected. When HeLa cells were treated with dexamethasone mesylate at 37 degrees C, and were subjected to crosslinking, electrophoresis of cytosolic glucocorticoid-receptor complexes was drastically reduced, whereas their levels in nuclear extracts were not appreciably altered.
Rossini
G P
GP
Istituto di Chimica Biologica, Università di Modena, Italy.
Malaguti
C
C
D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism*; D003600:Cytosol / Q000187:drug effects / Q000378:metabolism*; D003907:Dexamethasone / Q000031:analogs & derivatives / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D005976:Glutaral / Q000494:pharmacology; D006367:HeLa Cells; D006801:Humans; D011965:Receptors, Glucocorticoid / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*; D013696:Temperature
D011965:Receptors, Glucocorticoid; D003907:Dexamethasone; C031710:dexamethasone 21-methanesulfonate; D005976:Glutaral