Membrane-associated phospholipase A2 stimulates DNA synthesis in two murine fibroblasts.
The effect of human membrane-associated phospholipase A2 (M-PLA2) on DNA synthesis in fibroblasts was examined in order to clarify its biological function. Human M-PLA2 added exogenously to the fibroblast cell lines Swiss 3T3 and BALB/3T3 was found to augment their DNA synthesis. This stimulation of DNA synthesis was not affected by treatment with indomethacin. Our results suggest that human M-PLA2 itself has a mitogenic effect on fibroblasts independent of arachidonate products such as prostaglandins.
Kurizaki
T
T
Department of Surgery II, Kumamoto University Medical School, Japan.
Egami
H
H
Murata
K
K
Kiyohara
H
H
Okazaki
S
S
Yoshida
N
N
Ogawa
M
M
D016475:3T3 Cells / Q000187:drug effects; D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000201:enzymology; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis*; D004261:DNA Replication / Q000187:drug effects; D005347:Fibroblasts / Q000187:drug effects; D007213:Indomethacin / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D010741:Phospholipases A / Q000494:pharmacology*; D054467:Phospholipases A2; D011994:Recombinant Proteins / Q000494:pharmacology
D011994:Recombinant Proteins; D004247:DNA; D010741:Phospholipases A; D054467:Phospholipases A2; D007213:Indomethacin