The effect of dihydrotestosterone and culture conditions on proliferation of the human prostatic cancer cell line LNCaP.
Cell density, nutritional state, and serum factors modify the growth response of LNCaP human prostatic cancer cells to dihydrotestosterone. Evaluation of growth response to dihydrotestosterone requires logarithmic transformation of cell count or thymidine incorporation data. Under conditions of dose response, growth increases with cell density but no significant interaction of dihydrotestosterone with cell density was found under optimal culture conditions. The frequency of media change was a significant factor in modulating dose response. When cells from cultures maintained at different feeding periods were plated at different cell densities of (trypan blue) viable cells, significant effects of plating density on dihydrotestosterone response were found. Dihydrotestosterone protects cells under the adverse effects of media deprivation. Under the extreme adverse effects of serum deprivation, cells respond to dihydrotestosterone even under conditions of increasing cell loss. The effects of dihydrotestosterone on final cell density were significant. In the absence of serum, the elongated cells of LNCaP assume a round shape, but many remain adherent to the culture dish and can be restored to normal morphology by serum. A number of growth factors fail to restore normal morphology that was completely restored by a combination of fibronectin and dihydrotestosterone. We have not developed a practicable serum-free system for LNCaP.
Chen
H Z
HZ
Department of Neoplastic Diseases, Mount Sinai Medical Center, New York, New York 10029-6574.
Kirschenbaum
A
A
Mandeli
J
J
Hollander
V P
VP
D001685:Biological Factors / Q000494:pharmacology; D002452:Cell Count; D002455:Cell Division / Q000187:drug effects; D016895:Culture Media, Serum-Free; D013196:Dihydrotestosterone / Q000494:pharmacology*; D006801:Humans; D008297:Male; D009752:Nutritional Status / Q000502:physiology; D011471:Prostatic Neoplasms / Q000378:metabolism / Q000473:pathology*; D013936:Thymidine / Q000378:metabolism; D014407:Tumor Cells, Cultured
D001685:Biological Factors; D016895:Culture Media, Serum-Free; D013196:Dihydrotestosterone; D013936:Thymidine