Glomerular immune deposits in murine lupus models may contain histones.
Two types of lupus mice, NZB/NZW F1 female hybrids and mice with graft-versus-host disease (GVHD), were studied. Histones H3 and H2A were detected by immunofluorescence in glomeruli of 22/22 proteinuric GVHD and 8/12 proteinuric NZB/W F1 female mice; in non-proteinuric animals, 3/5 GVHD and 2/27 NZB/W F1 female were positive. Using antibodies to histone peptides it was shown that mainly the N-terminal regions of histones H3 and H2A were exposed in glomerular deposits. Western blot analysis revealed antibodies to histone subfractions in sera of 33/34 lupus mice that developed proteinuria. This study provides evidence that histones are involved in the pathogenesis of lupus nephritis.
Schmiedeke
T
T
Institute of Medical Microbiology, Freiburg, Germany.
Stoeckl
F
F
Muller
S
S
Sugisaki
Y
Y
Batsford
S
S
Woitas
R
R
Vogt
A
A
D000818:Animals; D000918:Antibody Specificity; D000936:Antigen-Antibody Complex / Q000737:chemistry*; D004195:Disease Models, Animal; D005260:Female; D005455:Fluorescent Antibody Technique; D006657:Histones / Q000032:analysis*; D007678:Kidney Glomerulus / Q000276:immunology*; D008181:Lupus Nephritis / Q000276:immunology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D008814:Mice, Inbred NZB
D000936:Antigen-Antibody Complex; D006657:Histones