title: Characterization of Chlorella virus PBCV-1 CviAII restriction and modification system.
abstract: A second DNA site-specific (restriction) endonuclease (R.CviAII) and its cognate adenine DNA methyltransferase (M.CviAII) were isolated from virus PBCV-1 infected Chlorella strain NC64A cells. R.CviAII, a heteroschizomer of the bacterial restriction endonuclease NlaIII, recognizes the sequence CATG, and does not cleave CmATG sequences. However, unlike NlaIII, which cleaves after the G and does not cleave either CmATG or mCATG sequences, CviAII cleaves between the C and A and is unaffected by mCATG methylation. The M.CviAII and R.CviAII genes were cloned and their DNA sequences were determined. These genes are tandemly arranged head-to-tail such that the TAA termination codon of the M.CviAII methyltransferase gene overlaps the ATG translational start site of R.CviAII endonuclease. R.CviAII is the first chlorella virus site-specific endonuclease gene to be cloned and sequenced.
authors.affiliation: Department of Plant Pathology, University of Nebraska, Lincoln 68583-0722.
authors.forename: Y
authors.initials: Y
authors.lastname: Zhang
authors.forename: M
authors.initials: M
authors.lastname: Nelson
authors.forename: J W
authors.initials: JW
authors.lastname: Nietfeldt
authors.forename: D E
authors.initials: DE
authors.lastname: Burbank
authors.forename: J L
authors.initials: JL
authors.lastname: Van Etten
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002708:Chlorella / Q000201:enzymology* / Q000235:genetics; D003001:Cloning, Molecular; D015252:Deoxyribonucleases, Type II Site-Specific / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics; D005814:Genes, Viral / Q000235:genetics*; D015698:Genomic Library; D008780:Methyltransferases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific) / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D015678:Viral Structural Proteins / Q000235:genetics*; D014780:Viruses / Q000201:enzymology / Q000235:genetics
chemical_list: D014764:Viral Proteins; D015678:Viral Structural Proteins; C051014:DNA modification methylase PBCVI; D008780:Methyltransferases; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific); C048422:endodeoxyribonuclease NlaIII; D015252:Deoxyribonucleases, Type II Site-Specific