Comparison of glycerolipid biosynthesis in homogenates from human, ovine, bovine and rat adipose tissue in vitro.
1. Assay conditions were compared for glycerolipid biosynthesis in homogenates prepared from human abdominal, ovine and bovine subcutaneous, and rat epididymal adipose tissues. 2. In contrast to other species, longer incubation time and greater homogenate concentration resulted in decreased glycerolipid biosynthesis with rat adipose tissue homogenates. 3. Species differences were observed in concentration-dependency for ATP and fatty acids (palmitate, oleate and palmitoleate). 4. Results indicated that glycerolipid biosynthesis transpired at different rates in the four species, and that ovine and human adipose tissue homogenates had similar properties.
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Department of Animal Science, University of Wyoming, Laramie, WY 82071.
D000255:Adenosine Triphosphate / Q000494:pharmacology; D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D004075:Diglycerides / Q000096:biosynthesis; D005227:Fatty Acids / Q000494:pharmacology; D005989:Glycerides / Q000096:biosynthesis*; D005994:Glycerophosphates / Q000494:pharmacology; D006801:Humans; D007231:Infant, Newborn; D007700:Kinetics; D008055:Lipids / Q000096:biosynthesis*; D008297:Male; D019308:Palmitic Acid; D010169:Palmitic Acids / Q000494:pharmacology; D010743:Phospholipids / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D012756:Sheep; D013045:Species Specificity; D014280:Triglycerides / Q000096:biosynthesis
D004075:Diglycerides; D005227:Fatty Acids; D005989:Glycerides; D005994:Glycerophosphates; D008055:Lipids; D010169:Palmitic Acids; D010743:Phospholipids; D014280:Triglycerides; D019308:Palmitic Acid; D000255:Adenosine Triphosphate