title: Fundus angiography with fluorescein-labelled peptide fraction from bovine factor VIII: correlation with histologic findings.
abstract: A peptide fraction (VUEFFE) obtained from bovine factor VIII, with a high affinity for vessel endothelium, was bound to fluorescein and used for angiography in rabbits. Several eyes had undergone laser photocoagulation on the retina. The location of the dye was studied histologically with fluorescence microscopy on the enucleated, freeze-dried eyes. The angiographic and histologic findings were compared with findings obtained using sodium fluorescein. Fluorescein-labelled VUEFFE gave a longer lasting fluorescence of the retinal vessels with histologic evidence of dye deposition on the vessel wall. It also showed marked affinity for the photocoagulated tissues, giving intense and prolonged staining of the laser burns. Possible clinical applications are discussed.
authors.affiliation: Department of Ophthalmology, University of Genova, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Cardillo Piccolino
authors.forename: D
authors.initials: D
authors.lastname: Ghiglione
authors.forename: P
authors.initials: P
authors.lastname: Ceppa
authors.forename: B
authors.initials: B
authors.lastname: Villaggio
authors.forename: F
authors.initials: F
authors.lastname: Carli
authors.forename: P
authors.initials: P
authors.lastname: Allegri
authors.forename: R
authors.initials: R
authors.lastname: Bertagno
authors.forename: M
authors.initials: M
authors.lastname: Zingirian
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D005169:Factor VIII; D019793:Fluorescein; D005451:Fluorescein Angiography; D005452:Fluoresceins; D005654:Fundus Oculi; D017075:Laser Coagulation; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D009842:Oligopeptides; D011817:Rabbits; D012164:Retinal Diseases / Q000175:diagnosis; D012171:Retinal Vessels / Q000473:pathology* / Q000601:surgery
chemical_list: D005452:Fluoresceins; D009842:Oligopeptides; D005169:Factor VIII; D019793:Fluorescein