[Comparison of homologous and heterologous standard curves using TR-FIA for the determination of total T4 in the rat].
A homologous standard curve for rat T4 measurement has been compared to heterologous standard curves, by a time resolved fluoroimmunoassay (TR-FIA). Homologous standard curves have been carried out starting from rat serum in the absence of thyroid hormone by using Samuel's technique. The security criteria (sensitivity, inter and intraassay precision, and accuracy), were measured and compared between the homologous and heterologous test. Analysis of variance and slopes between homologous and heterologous standard curves showed homogeneous variances, the differences in the slopes being not significant.
Lara
A
A
Departamento de Bioquímica, Facultad de Medicina, Universidad de Málaga, España.
López
J M
JM
García-Chacón
I
I
Morell
M
M
D000818:Animals; D015200:Fluoroimmunoassay / Q000379:methods*; D051381:Rats; D017208:Rats, Wistar / Q000097:blood*; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013974:Thyroxine / Q000097:blood*
D013974:Thyroxine
Comparación de rectas patrón homóloga y heteróloga mediante TR-FIA, para la determinación de T4 total en rata.