Analysis of Escherichia coli beta-galactosidase expression in transgenic mice by flow cytometry of sperm.
Alterations to the mammalian genome that occur during the development of germ cells, in particular during meiosis, can be introduced into the population upon fertilization. These alterations can occur through homologous recombination, genome rearrangement, or mutagenesis. Such events usually occur infrequently for any particular sequence. Because of the difficulty in analyzing a large number of offspring in a mammalian cross, we have developed a marker to detect these events in sperm, since a large number of these meiotic progeny are produced during male gametogenesis. We have expressed the Escherichia coli lacZ gene during spermatogenesis in transgenic mice and quantitated the levels of beta-galactosidase activity in single sperm with the fluorescence-activated cell sorter and a fluorogenic substrate, 5-dodecanoylaminofluorescein di-beta-D-galactopyranoside. Detection of rare positives was demonstrated in mixed sperm populations with as few as 0.01% positive sperm. Although the distribution of beta-galactosidase activity in caudal epididymal sperm populations is bimodal, it appears that beta-galactosidase, like other proteins that have been expressed postmeiotically, is distributed between transgene-positive and transgene-negative sperm.
Jasin
M
M
Program of Cell Biology and Genetics, Sloan-Kettering Institute, New York, NY.
Zalamea
P
P
D000818:Animals; D015139:Blotting, Southern; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D005260:Female; D005306:Fertilization; D005434:Flow Cytometry; D005798:Genes, Bacterial; D006720:Homozygote; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D015183:Restriction Mapping; D013091:Spermatogenesis; D013094:Spermatozoa / Q000201:enzymology*; D001616:beta-Galactosidase / Q000235:genetics* / Q000378:metabolism*
D001616:beta-Galactosidase