Receptor neuron losses result in decreased cytochrome P-450 immunoreactivity in associated non-neuronal cells of mouse olfactory mucosa.
Immunohistochemical and immunoblot analyses were used to investigate nasal cytochrome P-450 distribution in mice with either unilateral naris closure for 3, 4, or 5 months, or olfactory bulbectomy. P-450 immunoreactivity was observed only in the supporting cells and Bowman's glands of the olfactory mucosa. Immunoreactivity was clearly reduced in rostral regions of the open-side olfactory mucosa where losses of receptor neurons resulted from 3 to 5 months of closure. Closed-side immunoreactivity was similar to controls. In 4 month closure animals that had regrown their receptor neurons, open-side immunoreactivity was comparable to controls. Olfactory bulbectomy also depressed P-450 immunoreactivity. These data suggest that presence or absence of receptor neurons markedly affects P-450 expression in nonneuronal cells of the olfactory mucosa.
Walters
E
E
Division of Biological Sciences, University of Missouri, Columbia 65211.
Buchheit
K
K
Maruniak
J A
JA
D000818:Animals; D015153:Blotting, Western; D003577:Cytochrome P-450 Enzyme System / Q000276:immunology / Q000378:metabolism*; D007150:Immunohistochemistry; D051379:Mice; D009474:Neurons / Q000502:physiology*; D009830:Olfactory Bulb / Q000502:physiology; D009831:Olfactory Mucosa / Q000166:cytology / Q000201:enzymology* / Q000294:innervation; D011984:Sensory Receptor Cells / Q000502:physiology*; D012903:Smell / Q000502:physiology*
D003577:Cytochrome P-450 Enzyme System