title: RHO gene products, putative small GTP-binding proteins, are important for activation of the CAL1/CDC43 gene product, a protein geranylgeranyltransferase in Saccharomyces cerevisiae.
abstract: Two multicopy suppressors of the cal1-1 mutation in the yeast Saccharomyces cerevisiae have been isolated and characterized. They are identical to the yeast RHO1 and RHO2 genes, which encode putative small GTP-binding proteins. Multiple copies of either RHO gene suppressed temperature-sensitive growth of the cal1-1 mutant but did not suppress the cal1 null mutant. Genetic analysis suggests that overproduction of either RHO gene product acts for activation of the CAL1 gene product.
authors.affiliation: Department of Biology, Faculty of Science, University of Tokyo, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Qadota
authors.forename: I
authors.initials: I
authors.lastname: Ishii
authors.forename: A
authors.initials: A
authors.lastname: Fujiyama
authors.forename: Y
authors.initials: Y
authors.lastname: Ohya
authors.forename: Y
authors.initials: Y
authors.lastname: Anraku
mesh_terms: D019883:Alkyl and Aryl Transferases; D018797:Cell Cycle Proteins; D002687:Chitin Synthase; D004789:Enzyme Activation; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism*; D019204:GTP-Binding Proteins / Q000235:genetics* / Q000378:metabolism*; D005800:Genes, Fungal; D016153:Genes, Suppressor; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D009154:Mutation; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins; D014166:Transferases / Q000378:metabolism*; D020743:rhoB GTP-Binding Protein
chemical_list: D018797:Cell Cycle Proteins; D005656:Fungal Proteins; D008565:Membrane Proteins; D029701:Saccharomyces cerevisiae Proteins; D014166:Transferases; C069390:CHS3 protein, S cerevisiae; D002687:Chitin Synthase; D019883:Alkyl and Aryl Transferases; C083291:geranylgeranyltransferase type-I; C065203:CDC43 protein, S cerevisiae; D019204:GTP-Binding Proteins; D020743:rhoB GTP-Binding Protein