Pine stilbene synthase cDNA, a tool for probing environmental stress.
Stilbene synthase cDNAs were isolated from a pine (Pinus sylvestris) cDNA library. Poly(A)+RNA required for the preparation was obtained from young seedlings challenged with Botrytis cinerea. A full-length cDNA encoding pinosylvin-forming stilbene synthase was sequenced, and the deduced amino acid sequence was compared with sequences of resveratrol-forming stilbene synthases. The cDNA coding for the key enzyme of pinosylvin formation is a valuable tool for detecting early effects of environmental stress in pine needles.
Schwekendiek
A
A
University of Marburg, Department of Chemistry, Germany.
Pfeffer
G
G
Kindl
H
H
D000217:Acyltransferases / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D015342:DNA Probes / Q000235:genetics*; D017343:Genes, Plant / Q000235:genetics; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000382:microbiology*; D010944:Plants / Q000201:enzymology* / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis; D013267:Stilbenes / Q000378:metabolism; D000094162:Phytoalexins
D000217:Acyltransferases; D015342:DNA Probes; D012333:RNA, Messenger; D013267:Stilbenes; C049032:pinosylvin; D000094162:Phytoalexins; C022674:stilbene synthase