Proviral insertions near cyclin D1 in mouse lymphomas: a parallel for BCL1 translocations in human B-cell neoplasms.
By isolating genomic DNA clones that encompass the mouse Cyl-1 (cyclin D1) locus, we have identified a putative CpG island close to the 5' end of the gene. Pulsed-field gel electrophoresis with probes derived from either the 5' or 3' side of the CpG island established physical linkage to two independent markers on mouse chromosome 7, in a region that is syntenic with human chromosome 11q13. On the 3' side, Cyl-1 is approximately 75 kb from Hst-1 and Int-2, although there is an additional CpG island in the intervening DNA, while on the 5' side, Cyl-1 is less than 300 kb from Fis-1, an integration site for Friend murine leukaemia virus. As there is no intervening CpG island, proviral insertions at Fis-1 could influence the expression of Cyl-1 and we describe two virally induced tumours in which this appears to be the case. The data suggest that proviral insertions near Cyl-1 in mouse lymphomas are functionally equivalent to the BCL1 translocations that activate cyclin D1 expression in human B-cell malignancies.
Lammie
G A
GA
Molecular Oncology Laboratory, Imperial Cancer Research Fund, London, UK.
Smith
R
R
Silver
J
J
Brookes
S
S
Dickson
C
C
Peters
G
G
D000818:Animals; D015139:Blotting, Southern; D002874:Chromosome Mapping; D002880:Chromosomes, Human, Pair 11; D003360:Cosmids; D019938:Cyclin D1; D016213:Cyclins / Q000235:genetics*; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D004273:DNA, Neoplasm / Q000235:genetics / Q000302:isolation & purification; D005622:Friend murine leukemia virus / Q000235:genetics*; D015723:Gene Library; D008040:Genetic Linkage; D006801:Humans; D008223:Lymphoma / Q000235:genetics* / Q000382:microbiology; D016393:Lymphoma, B-Cell / Q000235:genetics*; D051379:Mice; D008807:Mice, Inbred BALB C; D015513:Oncogene Proteins / Q000235:genetics*; D009857:Oncogenes; D011533:Proviruses / Q000235:genetics*; D015183:Restriction Mapping; D014178:Translocation, Genetic
D016213:Cyclins; D004273:DNA, Neoplasm; D015513:Oncogene Proteins; D019938:Cyclin D1; D004247:DNA