Fluorimetric assay of D-lactate.
A fluorimetric assay for D-lactate in human blood samples was developed using an endpoint enzymatic assay with D-lactate dehydrogenase from Staphylococcus epidermidis. The intrabatch and interbatch coefficients of variance were 8.7% (n = 4) and 16.6% (n = 4), respectively. The limit of detection in blood was 3.73 nmol/ml. The assay suffers minor interference from S-D-lactoylglutathione, which was also present in the blood samples. The concentration of D-lactate in blood was (mean +/- SE, nmol/ml) normal healthy individuals, 11.0 +/- 1.2 (n = 7); and diabetic patients, 20.0 +/- 1.3 (n = 55) (a significant increase in diabetes mellitus; P < 0.01, Mann-Whitney U test).
McLellan
A C
AC
Department of Chemistry and Biological Chemistry, University of Essex, Wivenhoe Park, United Kingdom.
Phillips
S A
SA
Thornalley
P J
PJ
D002138:Calibration; D003922:Diabetes Mellitus, Type 1 / Q000097:blood; D003924:Diabetes Mellitus, Type 2 / Q000097:blood; D004795:Enzyme Stability; D005470:Fluorometry / Q000379:methods*; D006801:Humans; D007770:L-Lactate Dehydrogenase; D007773:Lactates / Q000097:blood*; D019344:Lactic Acid; D009243:NAD / Q000032:analysis; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D007773:Lactates; D009243:NAD; D019344:Lactic Acid; D007770:L-Lactate Dehydrogenase