Fast filtration enzyme immunoassay for haptens.
An immunometric method for determination of hapten concentration in fluids has been developed. High-affinity hapten-specific enzyme-labeled monoclonal antibodies are mixed with a sample containing hapten, then the mixture is filtered through a membrane with immobilized hapten. The level of enzyme activity retained by the membrane is inversely proportional to the concentration of hapten in a sample. The assay has been developed for theophylline, digoxin and phenobarbital. The coefficient of variation is less than 5% and the test takes about 2 min.
Danilova
N P
NP
Department of Medical Diagnostics, Institute of Biotechnology, Moscow, Russia.
Beckman
N I
NI
Yazynin
S A
SA
Vasilov
R G
RG
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D004077:Digoxin / Q000097:blood; D005374:Filtration; D006241:Haptens / Q000097:blood*; D006735:Horseradish Peroxidase / Q000276:immunology; D006801:Humans; D007124:Immunoenzyme Techniques; D051379:Mice; D010634:Phenobarbital / Q000097:blood; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013806:Theophylline / Q000097:blood
D000911:Antibodies, Monoclonal; D006241:Haptens; D004077:Digoxin; D013806:Theophylline; D006735:Horseradish Peroxidase; D010634:Phenobarbital