title: Noncolorimetric measurement of cell activity in three-dimensional histoculture using the tetrazolium dye 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide: the pixel image analysis of formazan crystals.
abstract: We describe a novel system for measuring the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction in three-dimensional histoculture which is no longer dependent on colorimetric determination of extracted formazan, but rather is based on a pixel image analysis of formazan crystals, and which allows intratumor heterogeneity to be taken into account. The MTT test is based on the enzymatic reduction of the tetrazolium salt 3-(4,5-dimethylthiazol-2-yl)-2,5-dipheniltetrazolium bromide to formazan crystals by living, metabolically active cells, but not in dead cells. The reaction was carried out in situ in six-well plates on gel-supported histocultured human tumors. After a 24-h incubation with different drugs the tumors were incubated with a solution of MTT. Frozen sections of the tumor pieces were made and the slides were then stained with a propidium iodide solution, whose fluorescence is proportional to the number of cells present. We demonstrate here that the formazan crystals, formed by MTT reduction, reflect polarized light and that this can be quantified by using an image analysis system based on bright-pixel quantitation directly on a frozen section of the original tissue. Combined with the use of the fluorescent dye propidium iodide, also measured by pixel analysis, we can express a ratio between the total amount of MTT reduction and the total number of cells present in the specimen that expresses the effect of drugs on the histocultured tumors. Since histology is well maintained in histoculture it is possible to take into account the heterogeneity present in the tumor with regard to drug response.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: AntiCancer, Incorporated, San Diego, California 92110.
authors.forename: D
authors.initials: D
authors.lastname: Colangelo
authors.forename: H Y
authors.initials: HY
authors.lastname: Guo
authors.forename: K M
authors.initials: KM
authors.lastname: Connors
authors.forename: L
authors.initials: L
authors.lastname: Silvestro
authors.forename: R M
authors.initials: RM
authors.lastname: Hoffman
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology; D004396:Coloring Agents; D003460:Crystallization; D004354:Drug Screening Assays, Antitumor / Q000379:methods*; D005562:Formazans; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007150:Immunohistochemistry; D013778:Tetrazolium Salts; D013844:Thiazoles; D014407:Tumor Cells, Cultured
chemical_list: D000970:Antineoplastic Agents; D004396:Coloring Agents; D005562:Formazans; D013778:Tetrazolium Salts; D013844:Thiazoles; C022616:thiazolyl blue