The effect of WEB 2086 on PAF-induced eosinophil chemotaxis and LTC4 production from eosinophils.
We investigated the effect of WEB 2086, a selective platelet-activating factor (PAF) receptor antagonist, on PAF-induced eosinophil chemotaxis and LTC4 production. WEB 2086 inhibited PAF-induced eosinophil chemotaxis in normals and asthmatics. To further determine if WEB 2086 is a selective PAF receptor antagonist, we examined the effect of WEB 2086 against formyl-methionyl-leucyl-phenylalanine (fMLP)-induced or eosinophil chemotactic factor of anaphylaxis (ECF-A)-induced eosinophil chemotaxis. WEB 2086 did not have a significant inhibition against fMLP or ECF-A-induced eosinophil chemotaxis. These results suggest that WEB 2086 is a selective and potent inhibitor of PAF-induced eosinophil chemotaxis and LTC4 production from eosinophils, due to its antagonism of PAF-receptors.
Miyagawa
H
H
Allergic Disease Center, Creighton University School of Medicine, Omaha, NE 68178.
Nabe
M
M
Hopp
R J
RJ
Okada
C
C
Bewtra
A K
AK
Townley
G
G
D000328:Adult; D001249:Asthma / Q000276:immunology; D001381:Azepines / Q000494:pharmacology*; D002631:Chemotactic Factors, Eosinophil / Q000494:pharmacology; D002634:Chemotaxis, Leukocyte / Q000187:drug effects*; D004804:Eosinophils / Q000187:drug effects* / Q000378:metabolism; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D010972:Platelet Activating Factor / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D013189:SRS-A / Q000096:biosynthesis*; D014230:Triazoles / Q000494:pharmacology*
D001381:Azepines; D002631:Chemotactic Factors, Eosinophil; D010972:Platelet Activating Factor; D013189:SRS-A; D014230:Triazoles; C052518:WEB 2086; D009240:N-Formylmethionine Leucyl-Phenylalanine