Human peripheral blood lymphocytes express D5 dopamine receptor gene and transcribe the two pseudogenes.
Sequential reverse transcription and polymerase chain reaction (RT-PCR) of the mRNA were used to investigate the expression of dopamine receptors in human peripheral blood lymphocytes. The RT-PCR products contained three types of sequences, each corresponding to those of the D5 dopamine receptor gene and the two related pseudogenes. The lymphocyte cDNA library also contained the clones encoding parts of the three genes. Binding profiles of dopaminergic ligands to the lymphocytes were similar to those for the native neuronal membranes.
Takahashi
N
N
Department of Neurology, Osaka University Medical School, Japan.
Nagai
Y
Y
Ueno
S
S
Saeki
Y
Y
Yanagihara
T
T
D001552:Benzazepines / Q000494:pharmacology; D001667:Binding, Competitive; D004298:Dopamine / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D015723:Gene Library; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D011544:Pseudogenes / Q000235:genetics*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011954:Receptors, Dopamine / Q000096:biosynthesis / Q000187:drug effects / Q000235:genetics*; D017447:Receptors, Dopamine D1; D050641:Receptors, Dopamine D5; D014158:Transcription, Genetic
D001552:Benzazepines; C493605:DRD5 protein, human; D012333:RNA, Messenger; D011954:Receptors, Dopamine; D017447:Receptors, Dopamine D1; D050641:Receptors, Dopamine D5; D004298:Dopamine