title: Osteogenic protein-2. A new member of the transforming growth factor-beta superfamily expressed early in embryogenesis.
abstract: Osteogenic protein-2, OP-2, a new member of the transforming growth factor-beta (TGF-beta) superfamily, closely related to the osteogenic/bone morphogenetic proteins, was discovered in mouse embryo and human hippocampus cDNA libraries. The TGF-beta domain of OP-2 shows 74% identity to OP-1, 75% to Vgr-1, and 76% to BMP-5, hence OP-2 may also have bone inductive activity. The genomic locus of OP-2 has seven exons, like OP-1, and spans more than 27 kilobases (kb). In the C-terminal TGF-beta domain, OP-2 has a unique additional cysteine. Mouse embryos express relatively high levels of OP-2 mRNA at 8 days, two species of 3 and 5 kb. A careful study of mRNA expression of the osteogenic proteins in specific organs revealed discrete mRNA species for BMP-3, BMP-4, BMP-5, and BMP-6/Vgr-1 in lung or liver of young and adult mice. OP-1 is expressed in kidney; however, OP-2 and BMP-2 mRNAs were not detected in any organs studied, suggesting an early developmental role.
authors.affiliation: Creative BioMolecules, Inc., Hopkinton, Massachusetts 01748.
authors.forename: E
authors.initials: E
authors.lastname: Ozkaynak
authors.forename: P N
authors.initials: PN
authors.lastname: Schnegelsberg
authors.forename: D F
authors.initials: DF
authors.lastname: Jin
authors.forename: G M
authors.initials: GM
authors.lastname: Clifford
authors.forename: F D
authors.initials: FD
authors.lastname: Warren
authors.forename: E A
authors.initials: EA
authors.lastname: Drier
authors.forename: H
authors.initials: H
authors.lastname: Oppermann
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000831:Animals, Newborn; D001483:Base Sequence; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D004622:Embryo, Mammalian; D005314:Embryonic and Fetal Development; D005091:Exons; D015870:Gene Expression; D015723:Gene Library; D006624:Hippocampus / Q000502:physiology*; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D005810:Multigene Family; D009838:Oligodeoxyribonucleotides; D009928:Organ Specificity; D011498:Protein Precursors / Q000235:genetics; D011506:Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D016212:Transforming Growth Factor beta / Q000235:genetics*
chemical_list: C525718:BMP2 protein, human; C405632:BMP8B protein, human; C525719:Bmp2 protein, mouse; C415983:Bmp8b protein, mouse; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins; D009838:Oligodeoxyribonucleotides; D011498:Protein Precursors; D011506:Proteins; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta; D004247:DNA