title: Measurement of phenytoin in serum using in-house reagents employing the Syva enzyme-multiplied immunoassay technique principle.
abstract: Reagents have been developed for the measurement of phenytoin in serum, using the enzyme-multiplied immunoassay approach first introduced by Syva. The assay uses a commercially available antiserum and phenytoin valeric acid coupled to glucose-6-phosphate dehydrogenase as the tracer. It has been adapted for use on an Eppendorf EPOS analyser, which allows 300 assays/h with high precision. Excellent agreement was achieved with commercial reagents, and costs were reduced markedly.
authors.affiliation: Department of Chemical Pathology, Medical College of St. Bartholomew's Hospital, London, England.
authors.forename: D L
authors.initials: DL
authors.lastname: Colbert
mesh_terms: D000906:Antibodies; D004355:Drug Stability; D017248:Enzyme Multiplied Immunoassay Technique; D006801:Humans; D010672:Phenytoin / Q000097:blood*; D011933:Reagent Kits, Diagnostic; D012015:Reference Standards; D012680:Sensitivity and Specificity
chemical_list: D000906:Antibodies; D011933:Reagent Kits, Diagnostic; D010672:Phenytoin