title: Production of normal young following insemination of frozen-thawed mouse spermatozoa into fallopian tubes of pseudopregnant females.
abstract: Mouse epididymal spermatozoa in the cryopreservation solution (18% raffinose and 3% skim milk in distilled water) were frozen and stored at -196 degrees C, and later thawed at room temperature. The thawed sperm suspension was inseminated into the Fallopian tubes containing ovulated oocytes in pseudopregnant females on the day of finding the vaginal plug. Five out of 12 females gave birth to 28 Young (5.6 per liter).
authors.affiliation: Nippon Institute for Biological Science, Tokyo, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Nakagata
mesh_terms: D000818:Animals; D015925:Cryopreservation / Q000662:veterinary*; D003451:Cryoprotective Agents; D005187:Fallopian Tubes; D005260:Female; D007315:Insemination, Artificial / Q000662:veterinary*; D008098:Litter Size; D008297:Male; D051379:Mice; D011555:Pseudopregnancy / Q000662:veterinary; D012098:Reproduction; D013094:Spermatozoa
chemical_list: D003451:Cryoprotective Agents