Chicken leg muscle alpha-connectin as studied by a monoclonal antibody to the 1200 kDa fragment.
Chicken leg gracilis muscle contained only alpha-connectin (ca 3000 kDa) without beta-connectin. When myofibrils were kept standing for 20 hr at 4 degrees C, alpha-connectin was degraded to beta-connectin (ca 2000 kDa) and 1200 kDa peptide. The latter was prepared from myofibrils and purified by gel filtration in the presence of SDS. A monoclonal antibody, alpha 7, to this 1200 kDa fragment was prepared. The antibody reacted with the 1200 kDa fragment and its mother molecule alpha-connectin, but not with beta-connectin. Immunoelectron microscopy using alpha 7, as well as other antibodies to chicken breast muscle beta-connectin, revealed that the 1200 kDa peptide covered the portion of alpha-connectin from the Z line to the N2 line region in the I band of chicken leg gracilis muscle sarcomeres. The results were in good agreement with those observed in rabbit skeletal muscle.
Ohtsuka
S
S
Department of Biology, Faculty of Science, Chiba University, Japan.
Kimura
S
S
Kawamura
Y
Y
Hirono
Y
Y
Maruyama
K
K
D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D001665:Binding Sites; D002645:Chickens; D064211:Connectin; D016253:Microscopy, Immunoelectron; D008970:Molecular Weight; D009124:Muscle Proteins / Q000276:immunology / Q000378:metabolism*; D009132:Muscles / Q000378:metabolism* / Q000648:ultrastructure; D009210:Myofibrils / Q000378:metabolism / Q000648:ultrastructure; D010446:Peptide Fragments / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D011494:Protein Kinases
D000911:Antibodies, Monoclonal; D064211:Connectin; D009124:Muscle Proteins; D010446:Peptide Fragments; D011494:Protein Kinases