title: Evaluation of penetrating keratoplasties with long-term cryopreserved corneas using simplified method.
abstract: Since March 1986, the authors have studied and simplified the corneal cryopreservation technique using a self-made QL Bio-freezer. On the basis of successful animal experiments, penetrating keratoplasties with human cadaver corneas cryopreserved 9 to 571 days (averaging 131 days) were performed in 60 patients (61 eyes). The patients were followed up for 1.5 to 50 months. 41 of the corneal grafts remained clear, 9 became translucent and 11 cloudy. A total of 46 eyes had their visual acuity improved significantly. The results show that clinical use of corneal cryopreservation by the simplified two-step method is noteworthy.
authors.affiliation: Department of Ophthalmology, Affiliated Hospital Qingdao Medical College.
authors.forename: C F
authors.initials: CF
authors.lastname: Wang
authors.forename: M C
authors.initials: MC
authors.lastname: Ju
authors.forename: Z R
authors.initials: ZR
authors.lastname: Shi
authors.forename: W R
authors.initials: WR
authors.lastname: Sun
authors.forename: M L
authors.initials: ML
authors.lastname: Jin
authors.forename: Y J
authors.initials: YJ
authors.lastname: Niu
authors.forename: J G
authors.initials: JG
authors.lastname: Liu
authors.forename: F Y
authors.initials: FY
authors.lastname: Ge
mesh_terms: D000293:Adolescent; D000328:Adult; D003315:Cornea; D015925:Cryopreservation / Q000379:methods; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D015948:Keratoplasty, Penetrating; D008297:Male; D008875:Middle Aged; D013997:Time Factors; D014021:Tissue Preservation