title: Presence of autoimmunity to pancreatic antigens in a patient with fibrocalculous pancreatic diabetes.
abstract: A case of fibrocalculous pancreatic diabetes (FCPD) is reported for which antibody and cellular immune characteristics were determined. The patient, a Thai woman, had serum islet cell antibodies (ICA) that were detected by both immunoperoxidase staining and an indirect enzyme-linked immunosorbent assay (ELISA). Serum anti-human insulin antibodies were negative by a displacement ELISA. Lymphoproliferation assay against pancreatic antigen prepared from a blood group O cadaveric donor was positive. Increased CD8+ lymphocytes were observed using direct immunofluorescence staining and flow cytometry. CD4+ T lymphocytes, B lymphocytes and NK cells were within normal levels. These findings provide evidence for autoimmunity to pancreatic antigens in a patient with fibrocalculous pancreatic diabetes.
authors.affiliation: Department of Microbiology, Faculty of Medicine, Siriraj Hospital, Bangkok, Thailand.
authors.forename: N
authors.initials: N
authors.lastname: Tandhanand-Banchuin
authors.forename: W
authors.initials: W
authors.lastname: Kespichayawatana
authors.forename: S
authors.initials: S
authors.lastname: Vannasaeng
authors.forename: C
authors.initials: C
authors.lastname: Banchuin
authors.forename: T
authors.initials: T
authors.lastname: Laohapand
authors.forename: S
authors.initials: S
authors.lastname: Panpimarnmas
authors.forename: K
authors.initials: K
authors.lastname: Pattanapanyasat
authors.forename: S
authors.initials: S
authors.lastname: Sarasombath
authors.forename: H K
authors.initials: HK
authors.lastname: Webster
mesh_terms: D000328:Adult; D000917:Antibody Formation; D001323:Autoantibodies / Q000097:blood*; D001327:Autoimmune Diseases / Q000276:immunology*; D002114:Calcinosis / Q000276:immunology*; D003922:Diabetes Mellitus, Type 1 / Q000188:drug therapy / Q000276:immunology*; D005260:Female; D006801:Humans; D007111:Immunity, Cellular; D007124:Immunoenzyme Techniques; D007328:Insulin / Q000627:therapeutic use; D007515:Islets of Langerhans / Q000276:immunology*; D008214:Lymphocytes / Q000276:immunology*; D010182:Pancreatic Diseases / Q000188:drug therapy / Q000276:immunology*; D013577:Syndrome
chemical_list: D001323:Autoantibodies; D007328:Insulin; C024211:islet cell antibody