title: [Affinity modification of the 40S subparticle from human placenta with derivatives of pAUG and pAUGU3].
abstract: Affinity labeling of 40S subunits from human placenta with 4-(N-2-chloroethyl-N-methylamino)benzylmethyl-[32P]phosphoamide s of oligoribonucleotides pAUG and pAUGU3 was studied. Covalent attachment of these derivatives to 40S subunits within the complexes with 40S subunits, formed in the presence of Met-tRNAf.eIF-2.GTP, was detected. Both rRNA and ribosomal proteins were modified. Fragments of 18S rRNA, containing sites of the reagent attachment were identified: 1058-1164 for pAUG derivative and 976-1057--for pAUG and pAUGU3 ones. The data obtained allowed to conclude that the presence of the neighbouring codon at the A-site, regardless of the presence of the tRNA in it, affects significantly the arrangement of the trinucleotide template in the codon-anticodon interaction region. The large subunit does not cause significant alterations in the structural organization of the codon-anticodon interaction region.
authors.forename: D A
authors.initials: DA
authors.lastname: Mundus
authors.forename: A A
authors.initials: AA
authors.lastname: Malygin
authors.forename: M A
authors.initials: MA
authors.lastname: Zenkova
authors.forename: M N
authors.initials: MN
authors.lastname: Repkova
authors.forename: A G
authors.initials: AG
authors.lastname: Ven'iaminova
authors.forename: S K
authors.initials: SK
authors.lastname: Smailov
authors.forename: A V
authors.initials: AV
authors.lastname: Li
authors.forename: B K
authors.initials: BK
authors.lastname: Iskakov
authors.forename: L V
authors.initials: LV
authors.lastname: Vratskikh
authors.forename: V I
authors.initials: VI
authors.lastname: Iamkovoĭ
mesh_terms: D000345:Affinity Labels; D000926:Anticodon; D015139:Blotting, Southern; D003062:Codon; D004275:DNA, Ribosomal / Q000378:metabolism; D005260:Female; D006801:Humans; D009588:Nitrogen Mustard Compounds / Q000737:chemistry; D009843:Oligoribonucleotides / Q000737:chemistry*; D009943:Organophosphorus Compounds / Q000737:chemistry; D010920:Placenta / Q000648:ultrastructure*; D011247:Pregnancy; D012358:RNA, Transfer, Met / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism*; D013698:Templates, Genetic
chemical_list: D000345:Affinity Labels; D000926:Anticodon; D003062:Codon; D004275:DNA, Ribosomal; D009588:Nitrogen Mustard Compounds; D009843:Oligoribonucleotides; D009943:Organophosphorus Compounds; D012358:RNA, Transfer, Met
vernacular_title: Affinnaia modifikatsiia 40S subchastits iz platsenty cheloveka proizvodnymi pAUG i pAUGU3.