Map-based cloning of a gene controlling omega-3 fatty acid desaturation in Arabidopsis.
A gene from the flowering plant Arabidopsis thaliana that encodes an omega-3 desaturase was cloned on the basis of the genetic map position of a mutation affecting membrane and storage lipid fatty acid composition. Yeast artificial chromosomes covering the genetic locus were identified and used to probe a seed complementary DNA library. A complementary DNA clone for the desaturase was identified and introduced into roots of both wild-type and mutant plants by Ti plasmid-mediated transformation. Transgenic tissues of both mutant and wild-type plants had significantly increased amounts of the fatty acid produced by this desaturase.
Arondel
V
V
MSU-DOE Plant Research Laboratory, Michigan State University, East Lansing 48824-1312.
Lemieux
B
B
Hwang
I
I
Gibson
S
S
Goodman
H M
HM
Somerville
C R
CR
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics*; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D044943:Fatty Acid Desaturases / Q000235:genetics*; D017343:Genes, Plant; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics
D010940:Plant Proteins; D044943:Fatty Acid Desaturases; C078565:omega-3 fatty acid desaturase