title: Use of ELISA to detect toxigenic Pasteurella multocida in atrophic rhinitis in swine.
abstract: The use of an enzyme-linked immunosorbent assay (ELISA) as a means of detecting dermonecrotoxin-producing strains of Pasteurella multocida was investigated. The assay was evaluated as a means to identify toxigenic P. multocida isolates recovered from nasal secretions of swine with atrophic rhinitis. The sensitivity and specificity of the ELISA for detecting dermonecrotoxin-producing P. multocida strains were compared to those of mouse-inoculation and cytotoxicity assays. The ELISA was highly sensitive and more specific than animal inoculation or tissue culture assay and is thus a more effective method for screening swine herds for the presence of toxigenic strains of P. multocida. The ELISA is a rapid, effective, economical way to identify toxigenic P. multocida isolates.
authors.affiliation: Department of Veterinary Pathobiology, School of Veterinary Medicine, Purdue University, W. Lafayette, IN 47907-1243.
authors.forename: T L
authors.initials: TL
authors.lastname: Bowersock
authors.forename: T
authors.initials: T
authors.lastname: Hooper
authors.forename: R
authors.initials: R
authors.lastname: Pottenger
mesh_terms: D000818:Animals; D001427:Bacterial Toxins / Q000096:biosynthesis; D003885:Dermotoxins / Q000096:biosynthesis; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary*; D009297:Nasal Mucosa / Q000382:microbiology; D010326:Pasteurella Infections / Q000662:veterinary*; D016979:Pasteurella multocida / Q000302:isolation & purification* / Q000378:metabolism; D015995:Prevalence; D012222:Rhinitis, Atrophic / Q000382:microbiology / Q000662:veterinary*; D012680:Sensitivity and Specificity; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology*
chemical_list: D001427:Bacterial Toxins; D003885:Dermotoxins; C052804:dermonecrotic toxin, Pasteurella multocida