[Confocal laser scanning microscopy for image cytometry].
By using a focused laser beam as the light source of the microscope, to minimized flare, together with a pinhole in front of a photo detector to eliminate out-of-focus data, the confocal laser scanning microscope (CLSM) provides a depth-discriminated fluorescence image with high spatial resolution. The CLSM, therefore, has been widely used as a tool for accurate morphometric and densitometric analyses. We discussed here the biological applications of CLSM for demonstrating the three-dimensional features of the embryonic heart tube and chromosome and for imaging fast dynamic changes of [Ca2+]i in the living heart muscle cell pairs.
Takamatsu
T
T
Department of Pathology, Kyoto Prefectural University of Medicine.
Fujita
S
S
D000818:Animals; D002118:Calcium / Q000378:metabolism; D002875:Chromosomes; D003592:Cytophotometry / Q000295:instrumentation*; D007834:Lasers; D008855:Microscopy, Electron, Scanning; D009206:Myocardium / Q000378:metabolism
D002118:Calcium