title: Lack of effect of parathyroid hormone on calcium homeostasis in rat platelets.
abstract: The in vitro effects of parathyroid hormone on Ca2+ handling by fura-2 loaded rat platelets were studied. The incubation of these platelets with rat parathyroid hormone (1-34) for 10 min had no effect on intracellular fura-2 metabolism or [Ca2+]i in the resting state. The [Ca2+]i response to 0.1 U/mL thrombin was unaffected by preincubation with parathyroid hormone in the presence or absence of extracellular Ca2+. Furthermore, the recovery rate of [Ca2+]i after the thrombin-induced peak in Ca(2+)-depleted media was not altered with parathyroid hormone. These data indicate that parathyroid hormone may not have a significant effect on Ca2+ homeostasis in rat platelets in unstimulated and stimulated conditions.
authors.affiliation: Department of Clinical Laboratory, Hiroshima University School of Medicine, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Oshima
authors.forename: T
authors.initials: T
authors.lastname: Tsubokura
authors.forename: H
authors.initials: H
authors.lastname: Matsuura
authors.forename: M
authors.initials: M
authors.lastname: Kainouchi
authors.forename: G
authors.initials: G
authors.lastname: Kajiyama
authors.forename: D A
authors.initials: DA
authors.lastname: McCarron
mesh_terms: D000818:Animals; D001792:Blood Platelets / Q000378:metabolism*; D002118:Calcium / Q000097:blood*; D005110:Extracellular Space / Q000378:metabolism; D016257:Fura-2; D006706:Homeostasis; D007425:Intracellular Membranes / Q000378:metabolism; D008297:Male; D010281:Parathyroid Hormone / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D010281:Parathyroid Hormone; D002118:Calcium; D016257:Fura-2