The 45 kDa and 27 kDa yeast's protein kinases are not immunologically related.
Two yeast casein kinase type-1 species of 45 kDa and 27 kDa (CK1) were purified to apparent homogeneity and used for investigation of their immunological affinity. Antisera against the two kinases were isolated; the antibody against the 45 kDa kinase did not react with the 27 kDa enzyme. The 27 kDa casein kinase was recognized only by its own antibody. The obtained data strongly suggest that the low molecular mass CK-1 is not a proteolytic product of the 45 kDa kinase species.
Szyszka
R
R
Department of Molecular Biology, Maria Curie-Sklodowska University, Lublin, Poland.
Tchórzewski
M
M
Dukowski
P
P
Winiarczyk
S
S
Gasior
E
E
D000908:Antibodies, Fungal / Q000032:analysis; D047388:Casein Kinases; D004591:Electrophoresis, Polyacrylamide Gel; D015151:Immunoblotting; D007527:Isoenzymes / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*; D008970:Molecular Weight; D010447:Peptide Hydrolases / Q000302:isolation & purification; D011494:Protein Kinases / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*; D012441:Saccharomyces cerevisiae / Q000201:enzymology*
D000908:Antibodies, Fungal; D007527:Isoenzymes; D011494:Protein Kinases; D047388:Casein Kinases; D010447:Peptide Hydrolases