Identification of nuclear factors which interact with the 5' flanking region of the EF-1 alpha O gene in Xenopus laevis.
The EF-1 alpha O gene of Xenopus laevis is a stage-specific gene, being transcribed in oogonia and oocytes, but not in postmeiotic germ cells and terminally differentiated cells. We found that two trans-acting factors from oocyte nuclear extract are able to interact with a DNA sequence in the 5'-upstream region of the EF-1 alpha O gene. Methylation interference experiments suggested that the two factors recognised the same DNA element. Gel retardation assays indicated that part of the protein binding site could be confined to a 21 bp sequence, located between -51 and -72, relative to the cap site. Interestingly, this region shares great homology to a negative regulatory segment in the promoter of the TFIIIA gene, another developmentally regulated gene.
Olesen
O F
OF
Division of Biostructural Chemistry, Arhus University, Denmark.
Frydenberg
J
J
D000818:Animals; D001483:Base Sequence; D004268:DNA-Binding Proteins / Q000502:physiology*; D005786:Gene Expression Regulation; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000502:physiology*; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D009865:Oocytes / Q000502:physiology; D010445:Peptide Elongation Factors / Q000235:genetics*; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D014982:Xenopus laevis / Q000235:genetics*
D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D009838:Oligodeoxyribonucleotides; D010445:Peptide Elongation Factors