title: Immunochemical characterization of a monoclonal antibody specific for Alzheimer's disease associated protein.
abstract: In this study, the monoclonal antibody PHF-1 which recognizes epitopes unique to Alzheimer's disease associated proteins (ADAP) has been characterized. Crossed affinity immunoelectrophoresis was used to estimate the binding constant for the interaction of PHF-1 with ADAP and to estimate the fraction of PHF-1 reactive protein. The binding constant of PHF-1 was determined to be 1.3 x 10(-8) M. Furthermore, the effect of dephosphorylation on the electrophoretic pattern of the PHF-1 reactive protein and the ensuing changes in its immunoreactivity were demonstrated.
authors.affiliation: Laboratory of Biochemical Genetics, National Institute of Mental Health, Washington, DC.
authors.forename: A
authors.initials: A
authors.lastname: Bodenteich
authors.forename: L G
authors.initials: LG
authors.lastname: Mitchell
authors.forename: C R
authors.initials: CR
authors.lastname: Merril
authors.forename: B E
authors.initials: BE
authors.lastname: Miller
authors.forename: H A
authors.initials: HA
authors.lastname: Ghanbari
authors.forename: N H
authors.initials: NH
authors.lastname: Heegaard
mesh_terms: D000544:Alzheimer Disease / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions; D000941:Antigens / Q000276:immunology*; D001921:Brain / Q000378:metabolism; D006801:Humans; D007122:Immunoelectrophoresis; D007150:Immunohistochemistry; D009419:Nerve Tissue Proteins / Q000276:immunology*; D012044:Regression Analysis
chemical_list: C056167:Alzheimer's disease antigen; D000911:Antibodies, Monoclonal; D000941:Antigens; D009419:Nerve Tissue Proteins