title: Identification of human myocard proteins separated by two-dimensional electrophoresis.
abstract: N-Terminal sequencing, internal sequencing and amino acid analysis were used to identify twelve proteins of the human myocard two-dimensional gel electrophoresis (2-DE) pattern. Amino acid analysis was shown to be a powerful tool in addition to sequencing. The identification of a disease-associated N-terminally blocked protein by internal sequencing was not successful. The twelve identified proteins are the basis of a human myocard 2-DE database.
authors.affiliation: Deutsches Herzzentrum Berlin, Germany.
authors.forename: P
authors.initials: P
authors.lastname: Jungblut
authors.forename: A
authors.initials: A
authors.lastname: Otto
authors.forename: V
authors.initials: V
authors.lastname: Regitz
authors.forename: E
authors.initials: E
authors.lastname: Fleck
authors.forename: B
authors.initials: B
authors.lastname: Wittmann-Liebold
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D016208:Databases, Factual; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D007526:Isoelectric Point; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009206:Myocardium / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D000596:Amino Acids; D011506:Proteins