In vitro fertilization, development, and implantation after exposure of mature mouse oocytes to visible light.
Mature mouse oocytes were exposed prior to in vitro fertilization to visible light during 1, 2, or 4 hr at an intensity of 4,000 lux. Compared to controls cultured under identical conditions but protected from light, exposed eggs did not show any significant modification of cleavage speed and rate. After transfer of blastocysts obtained in vitro in uteri of pseudopregnant females, the implantation rate and the proportion of normal fetuses were not found to be different in relation to preliminary light exposure of oocytes fertilized and cultured in vitro.
Barlow
P
P
IVF Clinic and Human Reproduction Research Unit, Saint Pierre Hospital, Free University of Brussels, Belgium.
Puissant
F
F
Van der Zwalmen
P
P
Vandromme
J
J
Trigaux
P
P
Leroy
F
F
D000818:Animals; D001755:Blastocyst / Q000528:radiation effects; D010064:Embryo Implantation / Q000528:radiation effects; D004624:Embryo Transfer; D005314:Embryonic and Fetal Development / Q000528:radiation effects; D005260:Female; D005307:Fertilization in Vitro; D008027:Light; D051379:Mice; D009865:Oocytes / Q000528:radiation effects*; D013997:Time Factors