Secretory pathway of beta/A4 amyloid protein precursor in familial Alzheimer's disease with Val717 to Ile mutation.
To determine whether the secretory pathway of beta/A4 amyloid protein precursor (APP) was altered in familial Alzheimer's disease (FAD) with a mutation of Val717 to Ile, cerebrospinal fluid was studied by Western blotting. The ratio of the density of the bands labeled with the antibody against the amino-terminal part of beta/A4 protein to that with the antibody against amino-terminal part of beta/A4 protein to that with the antibody against amino-terminal part of APP was not decreased. The present result suggests that the secretory pathway is not altered by the mutation in such a way that amyloidogenic full-length beta/A4 protein is generated.
Yanagisawa
K
K
Department of Neurology, School of Medicine, Tokyo Medical and Dental University, Japan.
Ihara
Y
Y
Miyatake
T
T
D000544:Alzheimer Disease / Q000134:cerebrospinal fluid / Q000235:genetics / Q000378:metabolism*; D016564:Amyloid beta-Protein Precursor / Q000134:cerebrospinal fluid / Q000378:metabolism*; D015153:Blotting, Western; D002478:Cells, Cultured; D006801:Humans; D007532:Isoleucine / Q000378:metabolism*; D009154:Mutation; D009422:Nervous System Diseases / Q000235:genetics / Q000378:metabolism; D014633:Valine / Q000378:metabolism*
D016564:Amyloid beta-Protein Precursor; D007532:Isoleucine; D014633:Valine