Inhibition of Chlamydia trachomatis growth in mouse fibroblasts by liposome-encapsulated tetracycline.
The in-vitro susceptibility of Chlamydia trachomatis to liposome-encapsulated tetracycline was determined and compared with free tetracycline. Anionic, cationic and neutral small unilamellar liposomes were used in this study. Chlamydia-infected mouse fibroblast monolayers were continuously exposed to varying concentrations of antibiotic, incubated for 48 h and Giemsa stained. The minimum inhibitory concentration (MIC) for anionic, cationic and neutral liposomes containing tetracycline were 0.38, 0.08 and 0.04 mg/L, respectively. This was approximately 2, 10, and 20 times more efficient than free tetracycline (MIC, 0.79 mg/L). Neutral liposomes displayed no visible toxic side-effects on the host cells. When compared with free tetracycline, neutral liposomes were the most efficient for the delivery of inhibitory concentrations of tetracycline to chlamydia-infected mouse fibroblast L cell cultures.
al-Awadhi
H
H
Department of Microbiology and Immunology, George Washington University Medical Center, DC 20037.
Stokes
G V
GV
Reich
M
M
D000818:Animals; D002478:Cells, Cultured; D002692:Chlamydia trachomatis / Q000187:drug effects* / Q000254:growth & development; D004337:Drug Carriers; D005347:Fibroblasts / Q000187:drug effects / Q000382:microbiology*; D008081:Liposomes; D051379:Mice; D008826:Microbial Sensitivity Tests; D013752:Tetracycline / Q000008:administration & dosage* / Q000494:pharmacology
D004337:Drug Carriers; D008081:Liposomes; D013752:Tetracycline