title: A simple protocol for the automation of DNA cycle sequencing reactions and polymerase chain reactions.
abstract: Automated DNA sequencing methods using robotic workstations have been previously reported, however it is often an arduous task to import these technologies into a laboratory. We describe protocols making use of a Beckman Biomek 1000 robotic workstation to prepare polymerase chain reactions (PCRs) and "cycle sequencing" reactions to be performed in a Perkin Elmer Cetus System 9600 thermocycler. The combination of these two instruments allows a high throughput of PCR and DNA sequencing reactions. The programs described are freely available via anonymous file transfer protocol (FTP).
authors.affiliation: Institute for Molecular Genetics, Baylor College of Medicine, Houston, Texas 77030.
authors.forename: A B
authors.initials: AB
authors.lastname: Civitello
authors.forename: S
authors.initials: S
authors.lastname: Richards
authors.forename: R A
authors.initials: RA
authors.lastname: Gibbs
mesh_terms: D017104:Bacteriophage M13 / Q000235:genetics; D001483:Base Sequence; D004279:DNA, Viral / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012371:Robotics; D017422:Sequence Analysis, DNA / Q000295:instrumentation*
chemical_list: D004279:DNA, Viral