Neurofilament and tubulin mRNA expression in Schwann cells.
Feeding of elemental tellurium to weanling rats blocks synthesis of cholesterol (a major component of myelin), and causes demyelination of the sciatic nerve. Expression of mRNA for myelin-specific genes in Schwann cells is downregulated. We now demonstrate specificity for Schwann cell injury in that expression of mRNAs for neurofilament subunits and for class II beta-tubulin (parameters sensitive to axonal injury) is unaltered in neurons of the dorsal root ganglia. An unexpected result was that in tellurium-treated rats there was marked upregulation of expression of mRNAs coding for the light and medium neurofilament subunits ("neuron-specific" proteins) as well as that for class II beta-tubulin (the major neuronal beta-tubulin isotype) in Schwann cells. Expression of these "neuronal" mRNA species was also detected in distal stumps of transected nerves at times when Schwann cells were undergoing dedifferentiation.
Roberson
M D
MD
Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill 27599.
Toews
A D
AD
Goodrum
J F
JF
Morell
P
P
D000818:Animals; D015152:Blotting, Northern; D015536:Down-Regulation / Q000187:drug effects; D005260:Female; D017403:In Situ Hybridization; D009186:Myelin Sheath / Q000502:physiology; D016900:Neurofilament Proteins / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D012583:Schwann Cells / Q000378:metabolism*; D012584:Sciatic Nerve / Q000187:drug effects / Q000378:metabolism; D013691:Tellurium / Q000494:pharmacology; D014404:Tubulin / Q000096:biosynthesis*; D015854:Up-Regulation / Q000187:drug effects
D016900:Neurofilament Proteins; D012333:RNA, Messenger; D014404:Tubulin; D013691:Tellurium