[In vivo effect of interleukin-2 (IL-2) on immunopotentiation in irradiated mice].
This study's purpose was to evaluate the effect of IL-2 on the recovery from immunosuppressive state in vivo. Young adult mice (ddY) were rendered immunodeficient by whole body irradiation (300 rad). IL-2 (20000 u/head/day) was administered intraperitoneally for 5 days after irradiation. In the experiment (I) immunological parameters were investigated. In the IL-2-treated mice peripheral lymphocytes and neutrophils were increased, but there was no change in the T and B lymphocyte ratio. The proliferative responses of spleen cells to mitogens were improved in the IL-2-treated mice. A significant expansion of macrophages in the intraperitoneal cavity was demonstrated. In the experiment (II) irradiated mice were given peritonitis by 1 puncture with a 23 gauge needle through their ligated cecum. This peritonitis model was followed for 2 weeks after ligation and puncture of the cecum. In the mice with IL-2 pretreatment and antibiotic therapy, the survival rate was significantly improved, compared with mice that only received antibiotic therapy. Administration of carrageenan and anti-T cell monoclonal antibodies significantly decreased the survival rate. These results suggested macrophages as well as functional T cells were necessary to decrease the mortality rate. In conclusion, IL-2 may have a remarkably protective effect against infection in immunodeficient state.
Toyoizumi
S
S
Second Department of Surgery, Chiba University, School of Medicine, Japan.
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D001424:Bacterial Infections / Q000517:prevention & control; D007108:Immune Tolerance / Q000528:radiation effects; D007376:Interleukin-2 / Q000494:pharmacology*; D008213:Lymphocyte Activation; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D010538:Peritonitis / Q000517:prevention & control; D014916:Whole-Body Irradiation
D000276:Adjuvants, Immunologic; D007376:Interleukin-2