Recombinant immunoblot assay for hepatitis C virus antibody in chronic hepatitis.
Testing for hepatitis C virus by ELISA requires confirmation by recombinant immunoblot assay (RIBA). The first-generation RIBA uses the same antigen as used in the ELISA and one further antigen. A second-generation RIBA in which two further antigens are present, detects positivity that is not found by either the ELISA or the original RIBA. Consequently, although it is adequate to test ELISA positive sera with the first-generation RIBA, the second-generation assay is recommended for confirming negativity.
Taliani
G
G
Institute of Tropical Diseases, La Sapienza University, Rome, Italy.
Badolato
M C
MC
Lecce
R
R
De Bac
C
C
De Marzio
E
E
Balsano
C
C
Artini
M
M
Levrero
M
M
D015153:Blotting, Western / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay; D006508:Hepatitis Antibodies / Q000302:isolation & purification*; D006526:Hepatitis C / Q000175:diagnosis*; D006521:Hepatitis, Chronic / Q000276:immunology*; D006801:Humans; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity
D006508:Hepatitis Antibodies; D011933:Reagent Kits, Diagnostic