title: Evaluation of greyhound susceptibility to malignant hyperthermia using halothane-succinylcholine anesthesia and caffeine-halothane muscle contractures.
abstract: We investigated Greyhounds because of prior reports of malignant hyperthermia (MH) episodes and because Greyhounds may express high genetic relatedness due to inbreeding for generations. Seven Greyhound and six mongrel dogs were given halothane and succinylcholine anesthesia as a challenge to trigger MH. They also underwent semitendinosus muscle biopsy for contracture study with halothane and caffeine. Measurements in vivo of mixed venous and arterial blood gases, cardiac output by thermodilution, temperature, blood pressure, and pulse rate provided sequential data regarding whole body O2 consumption (product of cardiac output and arterial-mixed venous O2 content difference), acid-base status, and arterial CO2 tension. Greyhounds and mongrels had uniformly similar in vivo and in vitro responses, without evidence for MH. Contracture thresholds were higher than those reported for normal swine and humans (8 mM vs. 4 mM). Information on MH susceptibility in this breed is important for laboratory investigation in Greyhounds as well as to veterinary medicine in general. Neither mongrels nor this group of Greyhounds were obviously susceptible to MH. If all Greyhounds are genetically homologous, then Greyhounds may not be specifically MH susceptible. These findings overall may provide a protocol and baseline normal comparative data for determining MH susceptibility in dogs and other species.
authors.affiliation: Merck, Sharp & Dohme Research Laboratories, Somerville, NJ.
authors.forename: S B
authors.initials: SB
authors.lastname: Cosgrove
authors.forename: P H
authors.initials: PH
authors.lastname: Eisele
authors.forename: R W
authors.initials: RW
authors.lastname: Martucci
authors.forename: G A
authors.initials: GA
authors.lastname: Gronert
mesh_terms: D000769:Anesthesia, Inhalation / Q000662:veterinary*; D000818:Animals; D002110:Caffeine; D004198:Disease Susceptibility / Q000662:veterinary; D004283:Dog Diseases / Q000235:genetics*; D004285:Dogs; D020022:Genetic Predisposition to Disease; D006221:Halothane; D066298:In Vitro Techniques; D008305:Malignant Hyperthermia / Q000209:etiology / Q000235:genetics / Q000662:veterinary*; D009119:Muscle Contraction / Q000187:drug effects*; D013045:Species Specificity; D013390:Succinylcholine
chemical_list: D002110:Caffeine; D013390:Succinylcholine; D006221:Halothane