A repressor region in the human beta-myosin heavy chain gene that has a partial position dependency.
Expression of the human beta-myosin heavy chain (beta MHC) gene was studied by transient assay in culture and in situ by direct injection of plasmids into adult rat hearts. In this report we describe a unique repressor region located -326/-309 (5'-TTGGTGGTCGTGGTCAGT-3') of the human beta MHC gene that is conserved among the rat, rabbit, and human beta MHC genes. This sequence conferred repression onto heterologous promoters when the sequence was located 5' but not 3' to the promoters. This partial positional dependency suggests that the factor may act by limiting the binding of enhancers, located more proximally, to their DNA binding sites.
Edwards
J G
JG
University Heart Center, University of Arizona, Tucson.
Bahl
J J
JJ
Flink
I
I
Milavetz
J
J
Goldman
S
S
Morkin
E
E
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D015500:Chloramphenicol O-Acetyltransferase / Q000096:biosynthesis / Q000235:genetics; D015870:Gene Expression; D005809:Genes, Regulator; D006367:HeLa Cells; D006321:Heart / Q000502:physiology*; D006352:Heart Ventricles; D006801:Humans; D008156:Luciferases / Q000096:biosynthesis / Q000235:genetics; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009218:Myosins / Q000096:biosynthesis / Q000235:genetics*; D009838:Oligodeoxyribonucleotides; D010957:Plasmids; D011401:Promoter Regions, Genetic; D011817:Rabbits; D051381:Rats; D017384:Sequence Deletion; D012689:Sequence Homology, Nucleic Acid; D014162:Transfection
D009838:Oligodeoxyribonucleotides; D008156:Luciferases; D015500:Chloramphenicol O-Acetyltransferase; D009218:Myosins