Bovine haptoglobin: single radial immunodiffusion assay of its polymeric forms and dramatic rise in acute-phase sera.
Using purified bovine haptoglobin (Hp) and specific antisera, a single radial immunodiffusion (SRID) assay method has been developed to measure the serum Hp level in cattle. Bovine Hp is a highly polymerized protein showing heterogeneous molecular forms in serum. After treatment with cysteine or glutathione, Hp was partially reduced to a homogeneous form, suitable for SRID assay. This method gives values comparable to those obtained by hemoglobin-binding capacity assay, and has the advantage of being simple and convenient. Although serum Hp was not detectable in healthy cattle, it was found more than 50-fold after invasive surgery, indicating that Hp is a characteristic acute-phase protein in cattle.
Morimatsu
M
M
Department of Biochemistry, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
Sarikaputi
M
M
Syuto
B
B
Saito
M
M
Yamamoto
S
S
Naiki
M
M
D000210:Acute-Phase Reaction / Q000276:immunology / Q000662:veterinary*; D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology*; D003545:Cysteine / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D006242:Haptoglobins / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*; D005779:Immunodiffusion / Q000379:methods / Q000662:veterinary*
D006242:Haptoglobins; D003545:Cysteine