[Affinity modification of 80S ribosomes from human placenta with mRNA analogs--derivatives of oligouridylates with alkylating groups at the 3'-end].
2',3'-O-[4-(N-2-chloroethyl-N-methylamino)]benzylidene derivatives of oligoribonucleotides (Up)5U[32P]pC and (Up)11U[32P]pC were used for affinity labeling of 80S ribosomes from human placenta in the presence of cognate Phe-tRNA(Phe). The derivatives retained the ability of tRNA(Phe)-dependent binding with 80S ribosomes and within the corresponding complexes were cross-linked to 40S subunits (preferentially to 18S rRNA). Sites of the mRNA analogs attachment were identified by blot-hybridization of the modified rRNA with restriction fragments of corresponding rDNA. They are located within positions 1610-1747 and 1748-1865 for both analogs. These fragments are situated at the 3'-end region of 18S rRNA in a highly conserved part of eukaryotic small subunit rRNA secondary structure.
Bulygin
K N
KN
Graĭfer
D M
DM
Zenkova
M A
MA
Malygin
A A
AA
Iamkovoĭ
V I
VI
Karpova
G G
GG
D000345:Affinity Labels; D000477:Alkylating Agents; D015139:Blotting, Southern; D003432:Cross-Linking Reagents; D005260:Female; D006801:Humans; D007700:Kinetics; D009588:Nitrogen Mustard Compounds / Q000737:chemistry; D009690:Nucleic Acid Conformation; D009843:Oligoribonucleotides / Q000737:chemistry*; D010920:Placenta / Q000648:ultrastructure*; D011247:Pregnancy; D012333:RNA, Messenger / Q000737:chemistry*; D012270:Ribosomes / Q000737:chemistry*; D014500:Uracil Nucleotides / Q000737:chemistry*
D000345:Affinity Labels; D000477:Alkylating Agents; D003432:Cross-Linking Reagents; D009588:Nitrogen Mustard Compounds; D009843:Oligoribonucleotides; D012333:RNA, Messenger; D014500:Uracil Nucleotides; C027916:oligo(U); C015062:2',3'-(O)-(4-(N-2-chloroethyl-N-methylamino)benzylidene)uridine-5'-methylphosphate
Affinnaia modifikatsiia 80S ribosom iz platsenty cheloveka analogami mRNK--proizvodnymi oligouridilatov s alkiliruiushcheĭ gruppoĭ na 3'-kontse.