title: Isolation and characterization of two opioid peptides from a bovine hemoglobin peptic hydrolysate.
abstract: Two opioid peptides were isolated from a bovine hemoglobin hydrolysate, by use of gel permeation (GP) and reverse phase (RP) high performance liquid chromatography (HPLC). Their primary structure and accurate molecular weights, determined by amino acid analysis and fast atom bombardment (FAB) mass spectrometry, were identical to fragments 31-40 (LVV-hemorphin-7) and 32-40 (VV-hemorphin 7) of the beta-chain of bovine hemoglobin. The same fragments occur in human hemoglobin in positions 32-41 and 33-41 of the beta-chain, respectively. The opioid potency of these peptides, exhibited by use of electrically stimulated muscle of isolated guinea-pig ileum (GPI), were significant and comparable with some others previously described. In addition, the location of the two opioid peptides, VV-hemorphin-7 and LVV-hemorphin-7, revealed the existence of a "strategic zone" both in the bovine and human beta-chains of hemoglobin.
authors.affiliation: Laboratoire de Technologie des Substances Naturelles, Villeneuve D'Ascq, France.
authors.forename: J M
authors.initials: JM
authors.lastname: Piot
authors.forename: Q
authors.initials: Q
authors.lastname: Zhao
authors.forename: D
authors.initials: D
authors.lastname: Guillochon
authors.forename: G
authors.initials: G
authors.lastname: Ricart
authors.forename: D
authors.initials: D
authors.lastname: Thomas
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D004723:Endorphins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D006168:Guinea Pigs; D006454:Hemoglobins / Q000737:chemistry* / Q000302:isolation & purification; D006461:Hemolysis; D006868:Hydrolysis; D007082:Ileum / Q000187:drug effects / Q000502:physiology; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D009119:Muscle Contraction / Q000187:drug effects*; D009130:Muscle, Smooth / Q000187:drug effects / Q000502:physiology; D010446:Peptide Fragments / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D016339:Spectrometry, Mass, Fast Atom Bombardment; D014462:Ultrafiltration
chemical_list: D004723:Endorphins; D006454:Hemoglobins; D010446:Peptide Fragments