[Structural changes of chromatin during proto-oncogene activation by cycloheximide: dose-effect].
The level of expression of cellular proto-oncogens c-myc and c-fos in rat liver has been studied as a function of protein synthesis rate (cycloheximide dose). Activation of proto-oncogens has been established to be initiated by 50% inhibition of nuclear protein synthesis. This promotes a certain level in chromatin structural rearrangements which is manifested, in particular, in decreasing activity of chromatin cleavage by Ca2+, Mg(2+)-DNAase and increasing degree of chromatin condensation. A role of topoisomerase II in chromatin structural rearrangements during proto-oncogen activation is postulated.
Shevchenko
N A
NA
Spitkovskiĭ
D D
DD
Loginov
A S
AS
Makar'eva
E D
ED
Boĭkov
P Ia
PIa
D000818:Animals; D002118:Calcium / Q000378:metabolism; D002413:Cations, Divalent; D002467:Cell Nucleus / Q000378:metabolism; D002843:Chromatin / Q000737:chemistry* / Q000187:drug effects; D003513:Cycloheximide / Q000494:pharmacology*; D003851:Deoxyribonucleases / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D015870:Gene Expression; D016762:Genes, fos; D016259:Genes, myc; D008099:Liver / Q000378:metabolism; D008274:Magnesium / Q000378:metabolism; D008297:Male; D011487:Protein Conformation; D011519:Proto-Oncogenes; D051381:Rats
D002413:Cations, Divalent; D002843:Chromatin; D003513:Cycloheximide; D003851:Deoxyribonucleases; D008274:Magnesium; D002118:Calcium
Strukturnye izmeneniia khromatina v protsesse aktivatsii protonkogenov tsklogeksimidom: doza-éffekt.