[Analysis of adhesive proteins on the surface of platelets from the patients with lung cancer: studies in histological type and clinical stage].
Flow cytometry was used to detect platelet-associated fibrinogen (PA-Fbg), platelet-associated fibronectin (PA-FN) and platelet-associated thrombospondin (PA-TSP) on the surface membrane of platelets and plasma (P)-TSP in 30 patients with lung cancer (16 case of adenocarcinoma and 14 of squamous cell carcinoma). ELISA was used to analyze beta-TG and PF4. In the lung cancer group, beta-TG and PF4 were higher than those of a normal control group. PA-Fbg values were correlated with beta-TG and PF4 values. Each adhesive protein had a higher value in the patient than in the normal control group, and the degree of the increase was related to the progression of clinical disease stage. In the squamous cell carcinoma group, the P-TSP value was significantly elevated. Platelet size increased as the clinical stage of the disease progressed. These results suggest the following: 1. An increase in PA-Fbg can indicate the presence of activated platelets. 2. In patients with lung cancer, activated platelets appear in the blood, and their numbers increase as the clinical stage of the disease progresses. 3. Differences in histologic type led to differences in binding adhesive protein.
Soga
T
T
First Department of Internal Medicine, Kansai Medical University.
Nomura
S
S
Kido
H
H
Yamaguchi
K
K
Fukuroi
T
T
Yanabu
M
M
Kokawa
T
T
Yasunaga
K
K
D000230:Adenocarcinoma / Q000097:blood*; D001792:Blood Platelets / Q000737:chemistry*; D002294:Carcinoma, Squamous Cell / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay; D005340:Fibrinogen / Q000032:analysis; D005353:Fibronectins / Q000032:analysis; D005434:Flow Cytometry; D006801:Humans; D008175:Lung Neoplasms / Q000097:blood* / Q000175:diagnosis; D010980:Platelet Membrane Glycoproteins / Q000032:analysis; D019699:Thrombospondins
D005353:Fibronectins; D010980:Platelet Membrane Glycoproteins; D019699:Thrombospondins; D005340:Fibrinogen