Biosynthesis of modified peptidoglycan precursors by vancomycin-resistant Enterococcus faecium.
In the presence of bacitracin, vancomycin-resistant Enterococcus faecium (vanA phenotype) accumulate UDP-N-acetylmuramyl(UDP-Mur-NAc)-tetrapeptide and a UDP-MurNAc-depsipentapeptide containing lactate substituted for the carboxy-terminal-D-alanine residue. In an in vitro peptidoglycan polymerization assay, the modified precursors function and confer resistance to vancomycin.
Allen
N E
NE
Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, Indiana 46285.
Hobbs
J N
JN
Richardson
J M
JM
Riggin
R M
RM
D000595:Amino Acid Sequence; D004352:Drug Resistance, Microbial; D016984:Enterococcus faecium / Q000187:drug effects / Q000378:metabolism*; D008969:Molecular Sequence Data; D010457:Peptidoglycan / Q000096:biosynthesis* / Q000737:chemistry; D011498:Protein Precursors / Q000096:biosynthesis / Q000737:chemistry; D014538:Uridine Diphosphate N-Acetylmuramic Acid / Q000031:analogs & derivatives / Q000096:biosynthesis / Q000737:chemistry; D014640:Vancomycin / Q000494:pharmacology
D010457:Peptidoglycan; D011498:Protein Precursors; D014538:Uridine Diphosphate N-Acetylmuramic Acid; C002225:UDP-N-acetylmuramic acid pentapeptide; D014640:Vancomycin