Structure elucidation of sulphated oligosaccharides from recombinant human tissue plasminogen activator expressed in mouse epithelial cells.
Sulphated N-linked carbohydrate chains isolated from recombinant human tissue plasminogen activator expressed in mouse epithelial (C127) cells were analysed as oligosaccharide alditols by methylation analysis, liquid secondary ion mass spectrometry, and one- and two-dimensional 1H-NMR spectroscopy. The results demonstrate that the major component has the following novel structure: NeuAc-alpha 2-6Gal beta 1-4GlcNAc beta 1-2[NeuAc alpha 2-3Gal beta 1- 4GlcNAc beta 1-4]-Man alpha 1-3[NeuAc alpha 2-3(SO4-6)Gal beta 1- 4-GlcNAc beta 1-2Man alpha 1-6]-Man beta 1-4GlcNAc beta 1- 4[Fuc alpha 1-6]GlcNAc-o1.
Pfeiffer
G
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Biochemisches Institut am Klinikum der Universität, Giessen, FRG.
Stirm
S
S
Geyer
R
R
Strube
K H
KH
Bergwerff
A A
AA
Kamerling
J P
JP
Vliegenthart
J F
JF
D000818:Animals; D002240:Carbohydrate Sequence; D002460:Cell Line; D015870:Gene Expression; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D051379:Mice; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009844:Oligosaccharides / Q000737:chemistry; D010959:Tissue Plasminogen Activator / Q000737:chemistry* / Q000235:genetics
D009844:Oligosaccharides; D010959:Tissue Plasminogen Activator