Recombinant human tumor necrosis factor-beta entrapped in liposomes formed by a modification of the dehydration-rehydration method retains potent cytotoxic activity on L929 cells in vitro.
Recombinant human tumor necrosis factor-beta (rhTNF-beta) may be encapsulated with high efficiency in phosphatidylcholine and distearoylphosphatidylcholine liposomes, with entrapment values of 93.4% and 92.3%, respectively, by first entrapping the substance in multilamellar vesicles using a high solute-to-phospholipid ratio followed by freeze-drying and then rehydration. The entrapped cytokine retains potent cytotoxic activity on L929 cells in vitro, causing 100% cytotoxicity, equal to that of free rhTNF-beta at a concentration of about 5 x 10(-8) g/ml.
Tan
L
L
Department of Pharmacology, Faculty of Medicine, National University of Singapore, Kent Ridge.
Goh
C R
CR
Porter
A G
AG
D000818:Animals; D016923:Cell Death / Q000187:drug effects; D002460:Cell Line; D003681:Dehydration; D004337:Drug Carriers; D006801:Humans; D008081:Liposomes; D008233:Lymphotoxin-alpha / Q000008:administration & dosage* / Q000302:isolation & purification; D051379:Mice; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000302:isolation & purification
D004337:Drug Carriers; D008081:Liposomes; D008233:Lymphotoxin-alpha; D011994:Recombinant Proteins