title: Thrombin activity resides on LVAD Dacron inflow and outflow grafts.
abstract: Patients on left ventricular assist devices (LVADs) are at increased risk for thromboembolism, and they experience elevations in platelet release and thrombin activity indices during device implantation. The Dacron grafts that lead from the ventricle to the LVAD and back to the aorta may harbor thrombin activity and protect this thrombin from anticoagulant action. To investigate this possibility, specimens isolated from LVAD grafts at the time of device explantation or implantation were incubated with citrated platelet poor plasma (cPPP), cPPP and 2 U/ml of heparin, cPPP and 2 U/ml of recombinant hirudin (r-hirudin), or cPPP and 50 U/ml of heparin. Thrombin activity was measured by the increase in incubated cPPP fibrinopeptide A (FPA) concentrations over cPPP FPA levels, without material contact. A chromogenic substrate for thrombin verified the effects seen. Thrombin activity was found at comparable levels on both the inflow and outflow LVAD grafts at explantation. This activity was not inhibited by low concentrations of heparin, but 50 U/ml of heparin and 2 U/ml of r-hirudin reduced the activity. At graft implantation (after preclotting), thrombin activity was higher than at explantation, but susceptibility to inhibition by heparin was also significantly greater. These results confirm that the LVAD Dacron grafts harbor surface thrombin activity resistant to anticoagulation that may be a primary source for LVAD thrombogenicity.
authors.affiliation: Artificial Heart and Lung Program, University of Pittsburgh, Pennsylvania.
authors.forename: W R
authors.initials: WR
authors.lastname: Wagner
authors.forename: P C
authors.initials: PC
authors.lastname: Johnson
authors.forename: B V
authors.initials: BV
authors.lastname: Heil
authors.forename: K A
authors.initials: KA
authors.lastname: Thompson
authors.forename: R L
authors.initials: RL
authors.lastname: Kormos
authors.forename: B P
authors.initials: BP
authors.lastname: Griffith
mesh_terms: D006352:Heart Ventricles / Q000187:drug effects / Q000378:metabolism; D006353:Heart-Assist Devices / Q000009:adverse effects*; D006493:Heparin / Q000008:administration & dosage; D006629:Hirudins / Q000008:administration & dosage; D006801:Humans; D011093:Polyethylene Terephthalates; D013917:Thrombin / Q000378:metabolism*; D013923:Thromboembolism / Q000209:etiology
chemical_list: D006629:Hirudins; D011093:Polyethylene Terephthalates; D006493:Heparin; D013917:Thrombin