Analysis of IL-2 functional structure by multiple cysteine substitutions.
IL-2 has three cysteine residues. The cysteines at positions 58 and 105 of active IL-2 form an intramolecular disulfide bond while that at position 125 remains as a free form. To evaluate the importance of correct disulfide bond, mutant proteins (muteins) that have triple and double substitutions of cysteines with alanines, namely A58/105/125 and A58/125, were made by polymerase chain reaction method respectively. Thymidine incorporation assay on CTLL-2 cells showed that although these two muteins were only 0.5-2.0% as potent as that of wild type IL-2, they were 50-200 fold more active than A58, a mutein that has substitution of cysteine at position 58 with alanine. Binding inhibition study showed that the relative affinity of muteins A58/125 and A58/105/125 for high affinity IL-2 receptors was 5-25 fold higher than that of A58. These results suggest that the dramatic decrease in the activity of mutein A58 may result from the formation of an incorrect disulfide bond between the cysteines at positions 105 and 125.
Rong
Y
Y
Division of Cytokine Biology, Food and Drug Administration, Bethesda, MD 20892.
Lee
N
N
Liang
S M
SM
D001667:Binding, Competitive; D002460:Cell Line; D004261:DNA Replication / Q000187:drug effects; D006801:Humans; D007376:Interleukin-2 / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology*; D007700:Kinetics; D008970:Molecular Weight; D016297:Mutagenesis, Site-Directed; D010957:Plasmids; D016133:Polymerase Chain Reaction; D015375:Receptors, Interleukin-2 / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism / Q000494:pharmacology; D013329:Structure-Activity Relationship
D007376:Interleukin-2; D015375:Receptors, Interleukin-2; D011994:Recombinant Proteins