Tributyltin is a potent inducer of the heat shock response in human diploid fibroblasts.
Submicromolar concentrations of tributyltin (TBT), a commercially used organotin compound, were found to induce the expression of several stress proteins, most notably HSP89 and HSP70, in IMR-90 human diploid fibroblasts in a time- and dose-dependent manner. This induction can be demonstrated by quantitation of 1) synthesis of the heat shock proteins (HSPs), 2) relative abundance of mRNA of hsp70, and 3) transient expression of a human hsp70 promoter driven reporter gene. TBT also increased the abundance of mRNA of heme oxygenase, whereas heat shock was without effect. Analysis of protein binding to a consensus heat shock element (HSE) by electrophoretic mobility shift assay suggests that the induction of the heat shock response by TBT was attributable to activation of the heat shock transcription factor (HSTF).
Zhang
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Department of Biological Sciences, Rutgers University, Piscataway, New Jersey 08855-1059.
Liu
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D001483:Base Sequence; D004171:Diploidy; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology; D015870:Gene Expression; D006360:Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D013997:Time Factors; D014220:Trialkyltin Compounds / Q000494:pharmacology*
D006360:Heat-Shock Proteins; D014220:Trialkyltin Compounds; C011559:tributyltin