C-type natriuretic peptide in bovine chromaffin cells. The regulation of its biosynthesis and secretion.
We report here the regulation of the biosynthesis and the secretion of C-type natriuretic peptide (CNP) in cultured bovine chromaffin cells. The combined treatment with protein kinase A and -C activators induced a 6-fold increase of intracellular levels of CNP-(1-103). The biosynthesized CNP-(1-103) was co-released with its mature forms, typically CNP-(51-103), upon stimulation by nicotine or depolarizing agents. This confirms the neuropeptidic character of this third member of the natriuretic peptide family, which might act as a neuromodulator or neurotransmitter.
Babinski
K
K
Department of Pharmacology, Faculty of Medicine, University of Montréal, Qué, Canada.
Féthière
J
J
Roy
M
M
De Léan
A
A
Ong
H
H
D000313:Adrenal Medulla / Q000378:metabolism*; D000818:Animals; D009320:Atrial Natriuretic Factor / Q000378:metabolism; D002417:Cattle; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D005576:Colforsin / Q000494:pharmacology; D066298:In Vitro Techniques; D020098:Natriuretic Peptide, C-Type; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D011493:Protein Kinase C / Q000378:metabolism; D011494:Protein Kinases / Q000378:metabolism; D011498:Protein Precursors / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D009419:Nerve Tissue Proteins; D011498:Protein Precursors; D020098:Natriuretic Peptide, C-Type; D005576:Colforsin; D009320:Atrial Natriuretic Factor; D011494:Protein Kinases; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate