Developmental change in subcellular location of Bp-1 protein with an ability to interact with both identifier sequence and its brain-specific transcript, BC-1 RNA.
Identifier sequences are transcribed to generate a brain-specific BC-1 RNA present as a ribonucleoprotein particle in the dendrites and somata of neurons. This ribonucleoprotein particle contains an identifier sequence-binding protein (Bp-1 protein). We report here the purification of BC-1 RNA and demonstrate that Bp-1 protein interacts directly with the RNA. We also demonstrate an accumulation of Bp-1 protein in the nucleus of brain cells from mouse fetus and newborns that precedes the postnatal increase in BC-1 RNA. Cytoplasmic Bp-1 protein present in a complex with BC-1 RNA increases postnatally with a concomitant decrease in nuclear Bp-1 protein. These observations suggest that Bp-1 protein may play a role(s) in the synthesis and nuclear export of BC-1 RNA.
Kobayashi
S
S
Department of Biochemistry, School of Pharmaceutical Sciences, Toho University, Chiba, Japan.
Tokuno
T
T
Suzuki
K
K
Higashi
N
N
Goto
S
S
Anzai
K
K
D000375:Aging / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn; D001483:Base Sequence; D001921:Brain / Q000254:growth & development / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism; D004247:DNA / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D005333:Fetus; D051379:Mice; D008815:Mice, Inbred Strains; D008969:Molecular Sequence Data; D009474:Neurons / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D062085:RNA, Long Noncoding; D022661:RNA, Untranslated; D015183:Restriction Mapping; D012261:Ribonucleoproteins / Q000235:genetics / Q000378:metabolism*; D020736:Ribonucleoproteins, Small Cytoplasmic; D013347:Subcellular Fractions / Q000378:metabolism; D013379:Substrate Specificity; D014158:Transcription, Genetic
C076675:Bc1 long-non-coding RNA, mouse; D004268:DNA-Binding Proteins; D062085:RNA, Long Noncoding; D022661:RNA, Untranslated; D012261:Ribonucleoproteins; D020736:Ribonucleoproteins, Small Cytoplasmic; D004247:DNA