[Flow cytometric analysis of proliferative characteristics of human leukemic cells in bone marrows in vivo using bromodeoxyuridine (BrdU) and its monoclonal antibody].
We developed a new method for analyzing the proliferative characteristics of human acute leukemic cells in bone marrows in vivo using BrdU and its monoclonal antibody. The thirty minutes after a bolus BrdU infusion, a 1st bone marrow aspiration (BMP) was performed to calculate BrdU-LI. Then, a 150-minute continuous infusion of BrdU was started. After the infusion, a 2nd BMP was done to calculate the increasing rate of the BrdU-LI for 3 hours. Each of the bone marrow cells was analyzed by FCM. A mean value of 10.4% for BrdU-LI in 22 previously untreated patients was obtained. In 3 of the patients, Ts and Tpot ranged from 13.5 to 33.9 hours and 6.8 to 10.7 days respectively.
Takeda
Y
Y
3rd Dep. Med., Kanazawa University School of Medicine.
Kanno
M
M
Nakamura
S
S
D000208:Acute Disease; D000911:Antibodies, Monoclonal; D001854:Bone Marrow Cells; D001973:Bromodeoxyuridine / Q000276:immunology*; D002453:Cell Cycle; D004273:DNA, Neoplasm / Q000096:biosynthesis; D005434:Flow Cytometry; D006801:Humans; D007938:Leukemia / Q000378:metabolism / Q000473:pathology*
D000911:Antibodies, Monoclonal; D004273:DNA, Neoplasm; D001973:Bromodeoxyuridine