T-cell receptor identification of an oligodendrocyte-specific autoreactive cytotoxic T-cell clone without self restriction.
In addition to myelin basic protein (MBP) and proteolipid protein (PLP), oligodendrocyte (Od) membrane autoantigens, such as the glycoprotein M2/MOG, could participate in the pathogenesis of autoimmune demyelinating diseases of the central nervous system (CNS), such as experimental allergic encephalomyelitis (EAE) or multiple sclerosis (MS). We have described an Od-specific autoreactive and cytotoxic T-cell clone, named C2, which recognized M2/MOG without conventional MHC restriction. In order to analyse the Od/C2 interaction, we determined the alpha/beta T-cell receptor (TCR) variable region usages and structures of C2. Monoclonal antibody stainings of C2 and nucleotide sequences show that the alpha chain is composed of a V alpha 5 and a J alpha identical to J alpha 18BBM142 gene segments, and that the TCR beta chain is composed of V beta 17a, D beta 2.1 and J beta 2.2 gene segments indicating that C2 used a conventional alpha/beta TCR for M2/MOG recognition.
Jewtoukoff
V
V
Unité de Pathologie de l'Immunité, Faculté de Médecine Necker-Enfants Malades, Paris, France.
Amzazi
S
S
Lebar
R
R
Bach
M A
MA
Marche
P N
PN
D000595:Amino Acid Sequence; D000818:Animals; D015551:Autoimmunity / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000096:biosynthesis; D005434:Flow Cytometry; D051379:Mice; D008815:Mice, Inbred Strains; D008969:Molecular Sequence Data; D009836:Oligodendroglia / Q000276:immunology*; D016133:Polymerase Chain Reaction; D051381:Rats; D011917:Rats, Inbred Lew; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
D016693:Receptors, Antigen, T-Cell, alpha-beta; D004247:DNA