title: Isolation and characterization of immunoglobulin binding proteins from Staphylococcus intermedius and Staphylococcus hyicus.
abstract: 125-I-IgG binding activities were observed with 15 (17%) of 90 S. intermedius isolates from dogs and 39 (95%) of 41 S. hyicus isolates from pigs. Binding activities were not detected with S. hyicus isolates from cows. The IgG binding proteins of 2 S. intermedius, 2 S. hyicus, and protein A from S. aureus Cowan I were isolated from their cell surfaces. The proteins precipitated with IgG preparations from human, rabbit, pig, dog and horse, but not with IgG from cow, mouse and chicken. This indicated that these IgG binding proteins could be classified as type I receptors. In addition, the isolated proteins from all 3 staphylococcal species precipitated with polyclonal chicken anti-protein A antiserum. SDS-PAGE, Western blotting and gel isoelectric focussing of the proteins revealed numerous bands in the 42,000 D range and acid isoelectric points. The isoelectric point of the isolated proteins from both S. intermedius cultures was slightly more acidic than those from S. hyicus and S. aureus. The present results indicate a close functional and antigenic similarity, if not identity, between IgG binding proteins of S. intermedius and S. hyicus, and protein A of S. aureus.
authors.affiliation: Institut für Bakteriologie und Immunologie, Justus-Liebig-Universität Giessen.
authors.forename: R T
authors.initials: RT
authors.lastname: Greene
authors.forename: C
authors.initials: C
authors.lastname: Lämmler
mesh_terms: D000818:Animals; D002417:Cattle; D002473:Cell Wall / Q000737:chemistry; D004285:Dogs; D007074:Immunoglobulin G / Q000276:immunology*; D008222:Lymphokines / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*; D029584:Prostatic Secretory Proteins; D017452:Receptors, IgG / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*; D013210:Staphylococcus / Q000737:chemistry*; D013552:Swine
chemical_list: D007074:Immunoglobulin G; D008222:Lymphokines; D029584:Prostatic Secretory Proteins; D017452:Receptors, IgG; C043076:beta-microseminoprotein; C013059:immunoglobulin-binding factors