Determination of picogram levels of heptylphysostigmine in human plasma using high-performance liquid chromatography with fluorescence detection.
A sensitive (50 pg/ml) method for the determination of heptylphysostigmine in human plasma is described. The procedure is based on liquid-liquid extraction of the drug from buffered plasma, and analysis of the concentrated organic extract using high-performance liquid chromatography on a silica column, under normal-phase chromatographic conditions, with fluorescence detection. Physostigmine was used as an internal standard. The assay has been fully validated in the concentration range 50-2000 pg/ml and utilized for the analysis of clinical samples from subjects dosed with heptylphysostigmine.
Herold
M L
ML
Merck Sharp and Dohme Research Laboratories, West Point, PA 19486.
Constanzer
M L
ML
Matuszewski
B K
BK
D002800:Cholinesterase Inhibitors / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004563:Electrochemistry; D006801:Humans; D010830:Physostigmine / Q000031:analogs & derivatives* / Q000097:blood; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence
D002800:Cholinesterase Inhibitors; C052450:physostigmine heptyl; D010830:Physostigmine