Isolation of a 18,000-Da molecular weight form of parathyroid hormone-related protein from the rat Walker carcinosarcoma 256.
The hypercalcaemic Walker carcinosarcoma 256 is a rat model for humoral hypercalcaemia of malignancy (HHM). Tumour products such as parathyroid hormone-related proteins (PTHRP) and various growth factors appear to be responsible for this syndrome. Recently, PTHRP immunoreactivity has been detected in the conditioned medium of Walker tumour cells. The present report describes the isolation of a 18,000-Da molecular weight form of PTHRP from Walker tumour homogenates, by using a relatively simple immunoaffinity purification method. Our results suggest that this PTHRP form is similar to that purified from other HHM-related tumours.
de Miguel
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Laboratorio de la Unidad Metabólica, Fundación Jiménez Díaz, Madrid, Spain.
Esbrit
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D000818:Animals; D001681:Biological Assay; D015153:Blotting, Western; D002279:Carcinoma 256, Walker / Q000737:chemistry*; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D005260:Female; D008970:Molecular Weight; D009363:Neoplasm Proteins / Q000302:isolation & purification*; D044162:Parathyroid Hormone-Related Protein; D011506:Proteins / Q000302:isolation & purification*; D051381:Rats; D017208:Rats, Wistar
D009363:Neoplasm Proteins; D044162:Parathyroid Hormone-Related Protein; D011506:Proteins