Production and specificity of antisera raised against 25-hydroxyvitamin D3-[C-3]-bovine serum albumin conjugates.
In order to obtain specific antisera for use in the enzyme immunoassay of 25-hydroxyvitamin D3, three hapten-carrier conjugates having different lengths of bridges at the C-3 position were prepared from 25-hydroxyvitamin D3 by coupling with bovine serum albumin using the active ester method. The specificity of anti-25-hydroxyvitamin D3 antisera elicited in rabbits was tested by a cross-reaction study with closely related secosterols and by measuring the plasma levels of 25-hydroxyvitamin D3 by means of radioimmunoassay using tritium-labeled antigen. The results indicated that the specificity of the antisera obtained is higher than that of vitamin D-binding protein, and that some of these antisera are suitable for enzyme immunoassay.
Kobayashi
N
N
Faculty of Pharmaceutical Sciences, Kanazawa University, Japan.
Ueda
K
K
Kitahori
J
J
Shimada
K
K
D000818:Animals; D000918:Antibody Specificity / Q000276:immunology; D002112:Calcifediol / Q000032:analysis* / Q000737:chemistry / Q000276:immunology; D003429:Cross Reactions / Q000276:immunology; D004306:Dose-Response Relationship, Immunologic; D006241:Haptens / Q000737:chemistry*; D007106:Immune Sera / Q000276:immunology*; D007124:Immunoenzyme Techniques; D011817:Rabbits; D011863:Radioimmunoassay; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
D006241:Haptens; D007106:Immune Sera; D012710:Serum Albumin, Bovine; D002112:Calcifediol