Identification of a highly conserved region at the 5' flank of the phospholamban gene.
Phospholamban is a protein that regulates the activity of the sarcoplasmic reticulum Ca(2+)-ATPase. The rat phospholamban gene contains a single intron of 6.5 kilobases which interrupts the 5' untranslated region. Primer extension and nuclease mapping analysis identified a major transcription initiation site 87 nucleotides upstream of the first exon/intron junction. A highly conserved region was identified at the 5' flank of the phospholamban gene. This region contained a TATA motif at position -52 which bound nuclear extract, and a consensus CAAT motif at position -76. This highly conserved region may be important in the regulation of basal transcriptional activity.
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Peter Belfer Laboratory for the Molecular Biology of Heart Failure, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
Feldman
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D000818:Animals; D001483:Base Sequence; D002135:Calcium-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D000252:Calcium-Transporting ATPases / Q000378:metabolism; D002467:Cell Nucleus / Q000502:physiology; D005091:Exons; D005796:Genes; D006352:Heart Ventricles; D007438:Introns; D008969:Molecular Sequence Data; D009206:Myocardium / Q000378:metabolism; D009838:Oligodeoxyribonucleotides; D016133:Polymerase Chain Reaction; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D011817:Rabbits; D051381:Rats; D017207:Rats, Sprague-Dawley; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D016385:TATA Box; D014158:Transcription, Genetic; D000099211:Phospholamban
D002135:Calcium-Binding Proteins; D000252:Calcium-Transporting ATPases; D009838:Oligodeoxyribonucleotides; D012333:RNA, Messenger; D000099211:Phospholamban