title: The influence of challenge dose, duration of immunity, or steroid treatment on mucosal mast cells and on the distribution of sheep mast cell proteinase in Haemonchus-infected sheep.
abstract: The distribution of granule-specific sheep mast cell proteinase (SMCP), was assayed by immunocytochemistry and quantified by immunoassay in sheep immune to Haemonchus contortus. Repeated infection with Haemonchus larvae over 10-12 weeks induced a pronounced mucosal mastocytosis, including intraepithelial globule leukocytes (GL), which, 7 days after ceasing this dosing regime, was associated with the inability of incoming larvae to establish within the abomasal mucosa. Loss of this resistance, due to the cessation of stimulation with Haemonchus larvae 84 days previously or to treatment of sheep with corticosteroid, was associated with a marked decline in mast cell density and concentrations of SMCP in abomasal mucosal tissues. Nevertheless, larvae also failed to establish in immune sheep rested from challenge 42 days previously and in which mast cell counts were not significantly different from those of control sheep. A small, but significant, release of SMCP was demonstrated in gastric mucus from immune sheep following larval challenge, whereas little or no SMCP was detected in mucus from naïve animals.
authors.affiliation: Moredun Research Institute, Edinburgh.
authors.forename: J F
authors.initials: JF
authors.lastname: Huntley
authors.forename: G F
authors.initials: GF
authors.lastname: Newlands
authors.forename: F
authors.initials: F
authors.lastname: Jackson
authors.forename: H R
authors.initials: HR
authors.lastname: Miller
mesh_terms: D000018:Abomasum / Q000276:immunology / Q000473:pathology; D000305:Adrenal Cortex Hormones / Q000008:administration & dosage / Q000494:pharmacology*; D000818:Animals; D002452:Cell Count / Q000187:drug effects; D010450:Endopeptidases / Q000378:metabolism*; D005753:Gastric Mucosa / Q000276:immunology / Q000473:pathology; D006188:Haemonchiasis / Q000276:immunology / Q000378:metabolism / Q000662:veterinary*; D007109:Immunity; D008407:Mast Cells / Q000201:enzymology / Q000276:immunology* / Q000469:parasitology; D012756:Sheep; D012757:Sheep Diseases / Q000276:immunology* / Q000378:metabolism / Q000469:parasitology
chemical_list: D000305:Adrenal Cortex Hormones; D010450:Endopeptidases