[The cloning of fragments of the streptomycete plasmid pSG1912 as a part of the vector pUC19].
DNA fragments of plasmid pSG1912 isolated from Streptomyces globisporus 1912 have been cloned into vector pUC19 in the E. coli cells. Stability of inheritance of hybrid derivatives in enterobacteria has been studied. The clones where recombinant plasmids are stably inherited have been chosen. The detailed restriction maps of cloned DNA fragments of pSG1912 have been constructed.
Zaverukha
V B
VB
Polevoda
B V
BV
Polishchuk
L V
LV
Matseliukh
B P
BP
D000818:Animals; D002642:Chick Embryo; D003001:Cloning, Molecular / Q000379:methods*; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D004926:Escherichia coli / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics*; D010957:Plasmids / Q000235:genetics*; D011995:Recombination, Genetic / Q000235:genetics; D015183:Restriction Mapping; D012988:Soil Microbiology; D013302:Streptomyces / Q000235:genetics*
D004269:DNA, Bacterial
Klonirovanie fragmentov streptomitsetnoĭ plazmidy pSG1912 v vostave vektora pUC19.