Purification of rabbit, rat and mouse protein C with the use of monoclonal antibody to human protein C, PC01.
Ca(++)-dependent monoclonal antibody specific to gamma-carboxyglutamic acid (Gla) domain of protein C was produced. It did not cross-react to the other vitamin K-dependent plasma proteins but to protein C of the other species. Using this monoclonal antibody, PC01, rabbit (170 micrograms), rat (60 micrograms) and mouse (40 micrograms) protein Cs were isolated from 100 ml of their plasma by affinity chromatography. All of these protein Cs were two chain form linked by disulfide bond as well as human protein C and activated by thrombin-thrombomodulin complex. Rat and mouse protein Cs showed similar characters to human protein C. On the other hand rabbit protein C had different M(r) of heavy and light chains and showed lower anticoagulant activity compared with human protein C.
Kimura
M
M
Pharma Research Laboratories, Hoechst Japan Limited, Saitama.
Kurosawa-Ohsawa
K
K
Takahashi
M
M
Koyama
M
M
Tanaka
S
S
Matsuishi
T
T
D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D002846:Chromatography, Affinity; D003429:Cross Reactions; D006801:Humans; D051379:Mice; D008970:Molecular Weight; D011486:Protein C / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*; D011817:Rabbits; D051381:Rats; D013045:Species Specificity
D000911:Antibodies, Monoclonal; D011486:Protein C