title: Purine biosynthetic genes are required for cadmium tolerance in Schizosaccharomyces pombe.
abstract: Phytochelatins (PCs) are metal-chelating peptides produced in plants and some fungi in response to heavy metal exposure. A Cd-sensitive mutant of the fission yeast Schizosaccharomyces pombe, defective in production of a PC-Cd-sulfide complex essential for metal tolerance, was found to harbor mutations in specific genes of the purine biosynthetic pathway. Genetic analysis of the link between metal complex accumulation and purine biosynthesis enzymes revealed that genetic lesions blocking two segments of the pathway, before and after the IMP branchpoint, are required to produce the Cd-sensitive phenotype. The biochemical functions of these two segments of the pathway are similar, and a model based on the alternate use of a sulfur analog substrate is presented. The novel participation of purine biosynthesis enzymes in the conversion of the PC-Cd complex to the PC-Cd-sulfide complex in the fission yeast raises an intriguing possibility that these same enzymes might have a role in sulfur metabolism in the fission yeast S. pombe, and perhaps in other biological systems.
authors.affiliation: Plant Gene Expression Center, U.S. Department of Agriculture, Albany, California 94710.
authors.forename: D M
authors.initials: DM
authors.lastname: Speiser
authors.forename: D F
authors.initials: DF
authors.lastname: Ortiz
authors.forename: L
authors.initials: L
authors.lastname: Kreppel
authors.forename: G
authors.initials: G
authors.lastname: Scheel
authors.forename: G
authors.initials: G
authors.lastname: McDonald
authors.forename: D W
authors.initials: DW
authors.lastname: Ow
mesh_terms: D000265:Adenylosuccinate Synthase / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D002104:Cadmium / Q000494:pharmacology*; D004271:DNA, Fungal; D004352:Drug Resistance, Microbial / Q000235:genetics; D017353:Gene Deletion; D005800:Genes, Fungal; D005818:Genetic Engineering; D005978:Glutathione; D008667:Metalloproteins / Q000378:metabolism; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009154:Mutation; D010641:Phenotype; D054811:Phytochelatins; D010940:Plant Proteins / Q000378:metabolism; D011687:Purines / Q000378:metabolism*; D012568:Schizosaccharomyces / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D013440:Sulfides / Q000378:metabolism
chemical_list: D004271:DNA, Fungal; D008667:Metalloproteins; D010940:Plant Proteins; D011687:Purines; D013440:Sulfides; D002104:Cadmium; D054811:Phytochelatins; D000265:Adenylosuccinate Synthase; D005978:Glutathione