title: Vaccinia virus gene B1R encodes a 34-kDa serine/threonine protein kinase that localizes in cytoplasmic factories and is packaged into virions.
abstract: Vaccinia virus open reading frame B1R was expressed in E. coli and shown to encode a serine/threonine protein kinase which phosphorylated casein and calf thymus histones in vitro. A polyclonal rabbit antiserum was raised against a TrpE-B1R bacterial fusion protein and used to characterize the B1R gene product. Immunoprecipitation and immunoblotting analyses detected a 34-kDa polypeptide that was synthesized early during vaccinia virus infection and which was apparently stable since it was easily detectable 18 hr postinfection. Immunofluorescence demonstrated that this protein localizes in cytoplasmic virus factories, the sites of virus DNA replication. Immunoblotting of vaccinia virions showed that the enzyme is packaged into virus particles.
authors.affiliation: Sir William Dunn School of Pathology, University of Oxford, United Kingdom.
authors.forename: A H
authors.initials: AH
authors.lastname: Banham
authors.forename: G L
authors.initials: GL
authors.lastname: Smith
mesh_terms: D001483:Base Sequence; D002364:Caseins / Q000378:metabolism; D002451:Cell Compartmentation; D003593:Cytoplasm / Q000382:microbiology; D004926:Escherichia coli / Q000235:genetics; D005814:Genes, Viral / Q000235:genetics*; D006657:Histones / Q000378:metabolism; D007150:Immunohistochemistry; D008969:Molecular Sequence Data; D010766:Phosphorylation; D017346:Protein Serine-Threonine Kinases / Q000235:genetics* / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D013379:Substrate Specificity; D014158:Transcription, Genetic; D014616:Vaccinia virus / Q000201:enzymology / Q000235:genetics*; D014764:Viral Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification; D014771:Virion / Q000254:growth & development*
chemical_list: D002364:Caseins; D006657:Histones; D011993:Recombinant Fusion Proteins; C074058:VB1R protein, Vaccinia virus; D014764:Viral Proteins; D017346:Protein Serine-Threonine Kinases