title: Retention fluids of chronic sinusitis induce neutrophil adherence to microvascular endothelial cells.
abstract: The adherence of circulating leukocytes to the vascular endothelium is a critical step in the emigration of leukocytes through blood vessel walls to inflammatory lesions. The influence of nasal secretions on the adherence of neutrophils to the vascular endothelium was investigated using monolayers of human mucosal microvascular endothelial cells derived from the inferior turbinate. Preincubation of vascular endothelial cells with retention fluids from the maxillary sinus of the patients with chronic sinusitis showed increased neutrophil adherence. Recombinant IL-1 beta was also tested and found to induce adherence of neutrophils to human mucosal microvascular endothelial cells. However, no adhesive effect was observed with the nasal secretions of nasal allergy. An enzyme-linked immunosorbent assay detected considerable amounts of IL-1 beta in the chronic sinusitis retention fluids, while the amounts of IL-1 alpha and TNF-alpha were very low. The increased adhesion of the neutrophils by the retention fluids of chronic sinusitis was also neutralized by the incubation with anti-IL-1 beta antibody in a dose dependent manner. These findings suggest that IL-1 beta in the paranasal secretion of chronic sinusitis induces the adherence of neutrophils to vascular endothelium and subsequent infiltration of neutrophils in the paranasal sinuses, thus contributing to the persistence of chronic sinusitis.
authors.affiliation: Department of Otolaryngology, Faculty of Medicine, Kagoshima University, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Itoh
authors.forename: S
authors.initials: S
authors.lastname: Katahira
authors.forename: T
authors.initials: T
authors.lastname: Matsuzaki
authors.forename: M
authors.initials: M
authors.lastname: Ohyama
authors.forename: K
authors.initials: K
authors.lastname: Fukuda
mesh_terms: D002448:Cell Adhesion; D002478:Cells, Cultured; D002908:Chronic Disease; D004730:Endothelium, Vascular / Q000473:pathology*; D005122:Exudates and Transudates / Q000276:immunology / Q000502:physiology*; D006801:Humans; D007375:Interleukin-1 / Q000032:analysis / Q000502:physiology; D008833:Microcirculation / Q000473:pathology; D008858:Microscopy, Phase-Contrast; D009297:Nasal Mucosa / Q000378:metabolism / Q000473:pathology; D009504:Neutrophils / Q000473:pathology / Q000502:physiology*; D012852:Sinusitis / Q000276:immunology / Q000473:pathology / Q000503:physiopathology*; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis
chemical_list: D007375:Interleukin-1; D014409:Tumor Necrosis Factor-alpha