Changes with respect to time in the in vivo adsorption of plasma proteins onto artificial heart blood pumps.
The distribution of adsorbed plasma proteins (albumin, IgG, and fibrinogen) on 10 artificial heart blood pumps coated with 2 segmented polyurethanes was evaluated quantitatively after long-term in vivo experiments with goats to determine how the adsorption of plasma proteins on the pumps was affected by the kinds of biomaterials used, and by the pumping duration. The adsorbed plasma proteins on the materials were determined quantitatively using the iodine-125 conjugated antibody method. Microscopically, the adsorbed plasma proteins were marked by the gold colloid conjugated antibody method, and analyzed using a field emission scanning electron microscope. The macroscopic results showed that: 1) the adsorbed plasma proteins on KP-13 were more evenly and finely distributed than those on Cardiothane; 2) with KP-13, the adsorption of IgG and albumin at the center of the pumps was significantly less than in the peripheral areas, and the adsorbed IgG and albumin decreased significantly as the pumping duration increased; 3) in contrast, the adsorbed fibrinogen increased significantly with time; and 4) with Cardiothane, the tendencies for adsorbed IgG and albumin to decrease, and for adsorbed fibrinogen to increase, were less significant than with KP-13. Microscopically, the gold colloids marking plasma proteins were found to not cover the whole of the surface, but were found scattered randomly or in clusters, with no relationship observed between the distributions of the three plasma proteins.(ABSTRACT TRUNCATED AT 250 WORDS)
Mabuchi
K
K
Research Center for Advanced Science and Technology, University of Tokyo, Japan.
Nakamura
H
H
Imachi
K
K
Ijiri
K
K
Chinzei
T
T
Abe
Y
Y
Yonezawa
T
T
Sugawara
I
I
Nozawa
H
H
Imanishi
K
K
D000327:Adsorption; D000818:Animals; D001672:Biocompatible Materials; D001798:Blood Proteins / Q000378:metabolism*; D005340:Fibrinogen / Q000378:metabolism; D006041:Goats; D006354:Heart, Artificial / Q000009:adverse effects*; D007074:Immunoglobulin G / Q000097:blood; D008855:Microscopy, Electron, Scanning; D012709:Serum Albumin / Q000378:metabolism; D013499:Surface Properties; D013927:Thrombosis / Q000209:etiology / Q000517:prevention & control; D013997:Time Factors
D001672:Biocompatible Materials; D001798:Blood Proteins; D007074:Immunoglobulin G; D012709:Serum Albumin; D005340:Fibrinogen