Towards a two-dimensional database of common human proteins.
A protein pattern of common human proteins was constructed by comparing the two-dimensional gel electrophoresis (2-DE) protein patterns from five cell lines of different germ layers. Total cell lysate and the isolated and purified nuclei of each cell line were separated by parallel electrophoresis runs in a multiple casting system of highest reproducibility. The computerized image analysis of the digitized 2-DE gels revealed a master protein pattern for each cell line. By comparison of all master protein patterns a 2-DE protein map of only common human proteins was constructed as a basis for a new 2-DE database. In a first step we have started characterizing a number of spots by microsequencing, amino acid composition analysis, and mass spectroscopy.
Burggraf
D
D
Max-Planck-Institut für Biochemie, Martinsried, Germany.
Andersson
K
K
Eckerskorn
C
C
Lottspeich
F
F
D001798:Blood Proteins / Q000302:isolation & purification; D002460:Cell Line; D016208:Databases, Factual; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D006863:Hydrogen-Ion Concentration; D009124:Muscle Proteins / Q000302:isolation & purification; D009419:Nerve Tissue Proteins / Q000302:isolation & purification; D011506:Proteins / Q000302:isolation & purification*
D001798:Blood Proteins; D009124:Muscle Proteins; D009419:Nerve Tissue Proteins; D011506:Proteins