Evidence that the packaging signal for nodaviral RNA2 is a bulged stem-loop.
Flock house virus is an insect virus belonging to the family Nodaviridae; members of this family are characterized by a small bipartite positive-stranded RNA genome. The larger genomic segment, RNA1, encodes viral replication proteins, whereas the smaller one, RNA2, encodes coat protein. Both RNAs are packaged in a single particle. A defective-interfering RNA (DI-634), isolated from a line of Drosophila cells persistently infected with Flock house virus, was used to show that a 32-base region of RNA2 (bases 186-217) is required for packaging into virions. RNA folding analysis predicted that this region forms a stem-loop structure with a 5-base loop and a 13-base-pair bulged stem.
Zhong
W
W
Institute for Molecular Virology, University of Wisconsin, Madison 53706.
Dasgupta
R
R
Rueckert
R
R
D001483:Base Sequence; D002213:Capsid / Q000378:metabolism; D006860:Hydrogen Bonding; D007304:Insect Viruses / Q000378:metabolism* / Q000648:ultrastructure; D008969:Molecular Sequence Data; D009024:Morphogenesis; D009690:Nucleic Acid Conformation; D012367:RNA, Viral / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D017384:Sequence Deletion; D013329:Structure-Activity Relationship; D014779:Virus Replication
D012367:RNA, Viral