A consensus repeat sequence from the human insulin gene linked polymorphic region adopts multiple quadriplex DNA structures in vitro.
A hypervariable region consisting of repeats of a 14 base pair (bp) consensus sequence ACAGGGGT(G/C)(T/C)GGGG is located 363 bp upstream of the human insulin gene. Different repeat numbers of this oligonucleotide give rise to a polymorphism, and so this region is commonly known as the insulin gene linked polymorphic region (ILPR). Here we present evidence, based on the mobility in non-denaturing polyacrylamide gels of two dissimilarly sized oligonucleotides containing the ILPR consensus sequence, that this sequence can adopt a number of quadriplex DNA structures in vitro.
Hammond-Kosack
M C
MC
Department of Medicine, University of Birmingham, Queen Elizabeth Hospital, UK.
Docherty
K
K
D001483:Base Sequence; D016384:Consensus Sequence; D004247:DNA / Q000737:chemistry*; D006801:Humans; D007328:Insulin / Q000235:genetics*; D008956:Models, Chemical; D008957:Models, Genetic; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D011110:Polymorphism, Genetic; D012091:Repetitive Sequences, Nucleic Acid
D007328:Insulin; D009838:Oligodeoxyribonucleotides; D004247:DNA