Characterization of a novel T lymphocyte protein which binds to a site related to steroid/thyroid hormone receptor response elements in the negative regulatory sequence of the human immunodeficiency virus long terminal repeat.
We have previously identified a T lymphocyte protein which binds to a site within the LTR of the human immunodeficiency virus type 1 (HIV-1) and exerts an inhibitory effect on virus gene expression. The palindromic site (site B) recognized by this protein is related to the palindromic binding sites of members of the steroid/thyroid hormone receptor family. Here we characterize the T cell protein binding to this site as a 100 kD protein which is most abundant in T cells and which binds to site B as a 200 kD complex. This protein is distinct from other members of the steroid/thyroid hormone receptor family including the COUP protein which has a closely related DNA binding specificity.
Orchard
K
K
Chester Beatty Laboratories, Institute of Cancer Research, London, UK.
Lang
G
G
Collins
M
M
Latchman
D
D
D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D015152:Blotting, Northern; D051838:COUP Transcription Factor I; D004268:DNA-Binding Proteins / Q000378:metabolism; D016325:HIV Long Terminal Repeat; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes / Q000378:metabolism*; D011988:Receptors, Thyroid Hormone / Q000378:metabolism; D012045:Regulatory Sequences, Nucleic Acid; D012097:Repressor Proteins / Q000378:metabolism*; D013601:T-Lymphocytes / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism
D051838:COUP Transcription Factor I; D004268:DNA-Binding Proteins; C497589:NR2F1 protein, human; D015345:Oligonucleotide Probes; D011988:Receptors, Thyroid Hormone; D012097:Repressor Proteins; D014157:Transcription Factors