Incorporation of [14C] arachidonic acid into ovine conceptus and endometrial lipids.
The purpose of this study was to quantitate conceptus and endometrial incorporation of [14C]arachidonic acid (AA) into individual neutral and polar lipids. Endometrium and conceptuses from pregnant ewes and endometrium from nonbred ewes were collected 14 and 16 d after onset of estrus (d 0). Tissues were incubated for 8 h at 37 degrees C in medium containing 1 microCi of [14C]AA. Thin-layer chromatographic procedures were used to separate 12 lipids. Radioactivity was measured in each lipid, and the amount (ng) of [14C]AA incorporated into each lipid was calculated. Conceptuses and endometrium incorporated more [14C]AA into triacylglycerols than into any other lipid. Day and tissue type affected differentially (i.e., day X tissue interaction) the incorporation of [14C]AA into several lipids; d-14 conceptuses incorporated [14C]AA more actively than did any other day-tissue combination. Results indicate that triacylglycerols may be an important reservoir for conceptus and endometrial AA. The remarkable ability of d-14 conceptuses to incorporate [14C]AA into various lipids may be important for their accelerated elongation and active prostaglandin synthetic system.
Lewis
G S
GS
Department of Animal Science, Virginia Polytechnic Institute and State University, Blacksburg 24061-0306.
Wood
D L
DL
Caldwell
D W
DW
D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism*; D002250:Carbon Radioisotopes; D005314:Embryonic and Fetal Development / Q000502:physiology*; D004717:Endometrium / Q000378:metabolism*; D005260:Female; D050356:Lipid Metabolism; D011247:Pregnancy; D012756:Sheep
D002250:Carbon Radioisotopes; D016718:Arachidonic Acid