Induction of interleukin 6 synthesis in mouse glomeruli and cultured mesangial cells.
Interleukin 6 (IL-6) is an autocrine growth factor of cultured mesangial cells (MC) and intraglomerular IL-6 production is suggested to be closely associated with the pathogenesis of human mesangial proliferative glomerulonephritis (mesPGN). In this study, to elucidate the mechanisms regulating the intraglomerular production of IL-6, we examined what kinds of stimuli are significant in the induction of IL-6 synthesis in vitro and in vivo. Incubation of cultured mesangial cells with interleukin 1 (IL-1) or bacterial lipopolysaccharide (LPS) induced significant IL-6 production, and intravenous injection of IL-1 or LPS into normal BALB/c mice induced significant intraglomerular IL-6 mRNA expression. Furthermore, we indicated in this study that IL-6 mRNA expression was augmented in the glomeruli of mice with immune complex-mediated glomerulonephritis.
Iwano
M
M
First Department of Internal Medicine, Nara Medical University, Japan.
Dohi
K
K
Hirata
E
E
Horii
Y
Y
Shiiki
H
H
Ishikawa
H
H
D000818:Animals; D000936:Antigen-Antibody Complex / Q000502:physiology; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D005260:Female; D015870:Gene Expression / Q000502:physiology; D005920:Glomerular Mesangium / Q000737:chemistry / Q000166:cytology* / Q000378:metabolism*; D005921:Glomerulonephritis / Q000235:genetics / Q000503:physiopathology; D007375:Interleukin-1 / Q000494:pharmacology; D015850:Interleukin-6 / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D007678:Kidney Glomerulus / Q000737:chemistry / Q000166:cytology* / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D011994:Recombinant Proteins / Q000494:pharmacology
D000936:Antigen-Antibody Complex; D007375:Interleukin-1; D015850:Interleukin-6; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D011994:Recombinant Proteins