Cellular reaction on the intraperitoneal injection of four types of polylactide particulates.
Four types of polylactide particulates, P-L-LA 100, 250, 550 KD and a P-DL-LA 400 KD were injected into the peritoneal cavity of mice. The inflammatory reaction showed an increase in cell number (mainly neutrophilic granulocytes) up to 48 h after which the cell numbers decreased below the control (phosphate-buffered saline). All four polylactide particulates aggregated and intermingled with inflammatory cells. The aggregates remained throughout the investigation period of 6 months. Quantitative measurements showed that standardization of the particle form and size is essential. From this study and other experiments in which calcium phosphates and asbestos were injected intraperitoneally, it is concluded that the inflammatory response observed in the peritoneal cavity is related to the type of material injected and probably to form and size of the individual particles, but not to molecular weight.
van Sliedregt
A
A
Biomaterials Department, University Hospital, Leiden, The Netherlands.
Knook
M
M
Hesseling
S C
SC
Koerten
H K
HK
de Groot
K
K
van Blitterswijk
C A
CA
D000818:Animals; D007249:Inflammation / Q000139:chemically induced* / Q000473:pathology; D007274:Injections, Intraperitoneal; D008264:Macrophages / Q000648:ultrastructure*; D051379:Mice; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D008970:Molecular Weight; D009504:Neutrophils / Q000648:ultrastructure*; D010316:Particle Size; D010529:Peritoneal Cavity; D011091:Polyesters / Q000008:administration & dosage / Q000633:toxicity*
D011091:Polyesters; C033616:poly(lactide)