[HIV detection in the bone transplant with polymerase chain reaction].
In trauma surgery, bone transplantation was needed in nearly 15% of all operations for reconstruction of defects. A high risk of viral infection remains in transplantation, however, cocultivation procedures have shown that human immunodeficiency virus (HIV-1) resides in bone of HIV-infected persons. Safety guidelines for running bone banks were not always easy to follow, and physical or chemical procedures applied for disinfection failed to inactivate HIV-1. In the present study we show that the polymerase chain reaction technique is an appropriate and sensitive means of detecting the HIV-1 genome in bone material prior to transplantation and-used in this way-can help to diminish the risk of HIV-1 transmission via bone transplantation.
Merz
H
H
Abteilung Angewandte Molekularbiologie, Johannes Gutenberg-Universität, Mainz.
Müller
W E
WE
Müller
H
H
Röder
W
W
D016025:Bone Transplantation / Q000009:adverse effects*; D015658:HIV Infections / Q000517:prevention & control / Q000635:transmission*; D015497:HIV-1; D006801:Humans; D009104:Multiple Trauma / Q000601:surgery*; D016133:Polymerase Chain Reaction; D011183:Postoperative Complications / Q000517:prevention & control; D012307:Risk Factors
HIV-Nachweis im Knochentransplantat mit der Polymerasekettenreaktion (PCR).