Identification of an autosome to X chromosome translocation in the domestic cow.
Chromosomes were harvested from two fibroblast lines derived from a phenotypically normal cow and her albino daughter, both known to be heterozygous for an X/autosome translocation. QFH-banding after early BrdU incorporation identified the translocated autosomal material as chromosome 23 bands 13-25, and revealed that the centromeric portion of the translocated chromosome 23 had been retained. Fluorescent in situ hybridization of a BoLA Class I cDNA probe to the normal chromosome 23 and to the translocated autosomal material confirmed the identity of the translocation, and allowed a more precise sublocalization of the MHC in cattle than that previously reported.
Gallagher
D S
DS
Department of Veterinary Pathobiology, Texas A&M University, College Station 77843.
Basrur
P K
PK
Womack
J E
JE
D000818:Animals; D002417:Cattle / Q000235:genetics*; D002871:Chromosome Banding / Q000662:veterinary; D005260:Female; D017404:In Situ Hybridization, Fluorescence / Q000662:veterinary; D007621:Karyotyping / Q000662:veterinary; D008285:Major Histocompatibility Complex; D014178:Translocation, Genetic; D014960:X Chromosome