title: Synthetic substrates and inhibitors for serine proteases from lymphocytes, mast cells, semen, and blood.
abstract: The substrate specificity of several serine proteases from cytotoxic T lymphocytes, natural killer cells, mast cells, seminal fluid, and blood plasma has been determined with synthetic peptide thiobenzyl ester and p-nitroanilide substrates. Several new enzymatic activities have been discovered. A variety of inhibitors such as isocoumarins, trifluoromethyl ketones, and peptide chloromethyl ketones were used to study these enzymes and were found to be potent inhibitors.
authors.affiliation: School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta 30332.
authors.forename: J C
authors.initials: JC
authors.lastname: Powers
authors.forename: S
authors.initials: S
authors.lastname: Odake
authors.forename: T
authors.initials: T
authors.lastname: Ueda
authors.forename: D
authors.initials: D
authors.lastname: Hudig
authors.forename: H C
authors.initials: HC
authors.lastname: Graves
authors.forename: M
authors.initials: M
authors.lastname: Kamarei
authors.forename: C M
authors.initials: CM
authors.lastname: Kam
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D053804:Granzymes; D006801:Humans; D007700:Kinetics; D008214:Lymphocytes / Q000201:enzymology*; D008407:Mast Cells / Q000201:enzymology*; D051379:Mice; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000138:chemical synthesis / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D012661:Semen / Q000201:enzymology*; D012697:Serine Endopeptidases / Q000097:blood / Q000378:metabolism*; D015842:Serine Proteinase Inhibitors / Q000494:pharmacology*; D013379:Substrate Specificity
chemical_list: D009842:Oligopeptides; D011994:Recombinant Proteins; D015842:Serine Proteinase Inhibitors; D053804:Granzymes; D012697:Serine Endopeptidases; C509212:GZMA protein, human