title: Distribution of HLA-DQA1, -DQB1 and DRB1 alleles in black IDDM patients and controls from Zimbabwe.
abstract: We have used the XI Histocompatibility Workshop sequence-specific oligonucleotide probes to determine the DRB1, DQA1 and DQB1 genotypes by dot-blot hybridization of polymerase chain reaction (pcr)-amplified material from a homogenous black population in Zimbabwe. The DR4 subtype DRB1*0405, the DR3 subtype DRB1*0301, DQB1*0201 and DQB1*0302 and DQA1*0301 and DQA1*0501 were significantly increased in the IDDM group compared to the controls, whereas DRB1*11, DQB1*0602 and DQA1*0102 were significantly decreased. Taken together, the data show that susceptibility and resistance to IDDM are associated both with particular haplotypes and DQA1-DQB1 heterodimers without one or other being overriding.
authors.affiliation: Department of Immunology, London Hospital Medical College, U.K.
authors.forename: J M
authors.initials: JM
authors.lastname: Garcia-Pacheco
authors.forename: B
authors.initials: B
authors.lastname: Herbut
authors.forename: S
authors.initials: S
authors.lastname: Cutbush
authors.forename: G A
authors.initials: GA
authors.lastname: Hitman
authors.forename: W
authors.initials: W
authors.lastname: Zhonglin
authors.forename: M
authors.initials: M
authors.lastname: Magzoub
authors.forename: G F
authors.initials: GF
authors.lastname: Bottazzo
authors.forename: C
authors.initials: C
authors.lastname: Kiere
authors.forename: G
authors.initials: G
authors.lastname: West
authors.forename: D
authors.initials: D
authors.lastname: Mvere
mesh_terms: D000328:Adult; D000483:Alleles; D044383:Black People / Q000235:genetics*; D002648:Child; D003922:Diabetes Mellitus, Type 1 / Q000235:genetics* / Q000276:immunology; D004198:Disease Susceptibility / Q000276:immunology; D005787:Gene Frequency; D005802:Genes, MHC Class II; D020022:Genetic Predisposition to Disease; D006683:HLA-DQ Antigens / Q000235:genetics* / Q000276:immunology; D059848:HLA-DQ alpha-Chains; D059866:HLA-DQ beta-Chains; D006684:HLA-DR Antigens / Q000235:genetics* / Q000276:immunology; D059811:HLA-DRB1 Chains; D000949:Histocompatibility Antigens Class II / Q000235:genetics* / Q000276:immunology; D006801:Humans; D016133:Polymerase Chain Reaction; D015030:Zimbabwe
chemical_list: D006683:HLA-DQ Antigens; D059848:HLA-DQ alpha-Chains; D059866:HLA-DQ beta-Chains; C069050:HLA-DQA1 antigen; C069051:HLA-DQB1 antigen; D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains; C553502:HLA-DRB1*03:01 antigen; D000949:Histocompatibility Antigens Class II