Sequence and methylation in the beta/A4 region of the rabbit amyloid precursor protein gene.
Alzheimer's disease is characterized by the accumulation of the beta/A4 fragment of the amyloid precursor protein in the hippocampal regions of the brain. We report here the isolation of genomic clones carrying exons 15, 16 and 17 of the beta/A4 coding region of the rabbit amyloid precursor protein gene. The complete sequence of these exons predicts that all three peptides are identical to their human counterparts. An unexpectedly high concentration of CpG dinucleotides seen in exon 15 were conserved and continued into the intron 15 region. MspI/HpaII southern blot analysis revealed the presence of a number of methylated CpG dinucleotides in the cloned region of the gene. These data suggest that the rabbit amyloid precursor protein gene could provide a new and useful model for the study of this important gene.
Davidson
J S
JS
Southern Illinois University School of Medicine, Department of Medical Microbiology/Immunology, Springfield 62794-9230.
West
R L
RL
Kotikalapudi
P
P
Maroun
L E
LE
D000595:Amino Acid Sequence; D016564:Amyloid beta-Protein Precursor / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000235:genetics; D005091:Exons; D005796:Genes; D006801:Humans; D007438:Introns; D008099:Liver / Q000502:physiology; D008745:Methylation; D051379:Mice; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D011817:Rabbits; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
D016564:Amyloid beta-Protein Precursor; D015345:Oligonucleotide Probes; D004247:DNA