In vitro translation of a subgenomic mRNA from purified virions of the Spanish field isolate AST/89 of rabbit hemorrhagic disease virus (RHDV).
Purified preparations of the Spanish field isolate of rabbit hemorrhagic disease virus AST/89 were found to contain the plus-stranded genomic RNA of more than 7.4 kilobases (kb) and large amounts of a subgenomic mRNA of 2.4 kb. The smaller RNA was translated in vitro and shown to code for a 60 kDa protein which was immunoprecipitated using anti-RHDV as well as anti-VP60 sera.
Boga
J A
JA
Departamento de Biología Funcional (Area de Bioquímica), Universidad de Oviedo, Spain.
Marín
M S
MS
Casais
R
R
Prieto
M
M
Parra
F
F
D002139:Caliciviridae / Q000235:genetics* / Q000302:isolation & purification; D002474:Cell-Free System; D016679:Genome, Viral; D014176:Protein Biosynthesis; D015347:RNA Probes; D012333:RNA, Messenger / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics; D014771:Virion / Q000235:genetics / Q000302:isolation & purification
D015347:RNA Probes; D012333:RNA, Messenger; D012367:RNA, Viral; D014764:Viral Proteins