Urinary excretion of furosemide in rats with HgCl2-induced acute renal damage.
To examine the influence of mercuric chloride (HgCl2)-induced acute renal damage on urinary excretion of furosemide, HgCl2 (1 mg/kg) or its vehicle alone was given intraperitoneally to Wistar rats. The following two experiments were done. Study I: Three percent body weight (b.w.) of 1% NaCl solution or furosemide (30 mg/kg) in 3% b.w. of 1% NaCl solution was given orally before and after HgCl2 treatment, and an 8-hour urine was collected. Study II: Furosemide (30 mg/kg) was given orally, and blood samples were obtained at 1, 2, 3, 4, 6 and 8 hours after administration. Urinary excretion of N-acetyl-beta-D-glucosaminidase increased, and urine volume and urinary excretions of furosemide and sodium decreased in the HgCl2-treated rats. There were significant correlations between the urinary furosemide and its diuretic effects. Regression lines after HgCl2 were significantly different from those before treatment. The values of absorption as well as elimination rate constant were smaller, while the time to maximum concentration and the elimination half-life were longer in the HgCl2-treated rats compared to vehicle-treated animals. These results suggest that the urinary excretion of furosemide and the responsiveness of renal tubular cells to this agent are impaired in rats with HgCl2-induced acute renal damage.
Fujimura
A
A
Department of Clinical Pharmacology, Jichi Medical School, Tochigi, Japan.
Sudoh
T
T
Ohashi
K
K
Ebihara
A
A
D058186:Acute Kidney Injury / Q000097:blood / Q000139:chemically induced / Q000652:urine*; D000818:Animals; D005665:Furosemide / Q000097:blood / Q000493:pharmacokinetics / Q000652:urine*; D008297:Male; D008627:Mercuric Chloride / Q000506:poisoning*; D051381:Rats; D017208:Rats, Wistar; D012044:Regression Analysis; D013997:Time Factors
D008627:Mercuric Chloride; D005665:Furosemide