title: Effect of age and intake on growth hormone kinetics in dairy heifers.
abstract: The effects of aging and intake on growth hormone (GH) kinetics and GH-releasing factor (GRF)-induced GH concentrations were studied in two groups of 12 Holstein heifers each (80 d, 85 kg: young; and 273 d of age, 246 kg: old). Each group was then equally subdivided into full-fed (FF) and restricted-fed (RF) subgroups. After 11 d of intake treatment, animals were infused for 3 hr with GH (1.5 mg/hr) in order to calculate GH metabolic clearance rate (MCR), secretion rate (SR) and half-life (t 1/2). Two d later, total plasma volume was determined and the following day, all heifers received a GRF challenge (5 micrograms/kg i.v.). The following values are LSM +/- SE for young-FF, young-RF, old-FF and old-RF. Rate of secretion was not affected by any treatment, averaging 1.51, 1.25, 1.34, and 1.40 +/- .23 micrograms/min. Aging increased (P < .01) MCR (186, 159, 382, and 300 +/- 21 ml/min) and increased plasma volume (P < .01), which resulted in lower basal GH concentrations. Aging also decreased (P < .01) the area under the GH response curve following GRF injection (AUC: 12442, 21114, 5155, and 6308 +/- 1776 ng.min/ml) but did not affect average GH quantity in the plasma after the GRF challenge. Feed restriction decreased (P < .05) MCR, but not enough to affect basal GH concentrations.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Agriculture Canada, Station de recherches Lennoxville, Québec.
authors.forename: H
authors.initials: H
authors.lastname: Lapierre
authors.forename: C
authors.initials: C
authors.lastname: Farmer
authors.forename: C
authors.initials: C
authors.lastname: Girard
authors.forename: P
authors.initials: P
authors.lastname: Brazeau
mesh_terms: D000375:Aging / Q000097:blood*; D000818:Animals; D002417:Cattle / Q000097:blood*; D005260:Female; D005508:Food Deprivation / Q000502:physiology*; D013006:Growth Hormone / Q000097:blood*; D007700:Kinetics; D017337:Sermorelin
chemical_list: D017337:Sermorelin; D013006:Growth Hormone