Incorporation of 15N from L-leucine into isoleucine by rat brain cerebral cortex slices.
The fate of leucine nitrogen in the central nervous system was investigated by incubating rat cerebral cortex slices in the presence of 0.5 mM each of L-[15N]-leucine, L-isoleucine, and L-valine. Analysis of the slices and incubation media for free amino acids by gas chromatography-mass spectrometry revealed that the 15N from leucine is incorporated into isoleucine only. No 15N was detected in valine or any other amino acid. These results suggest that leucine, valine, and their corresponding aminotransferases may be compartmentalized in brain cerebral cortex.
Gheorvassaki
E
E
Laboratory of Biological Chemistry, School of Medicine, University of Athens, Greece.
Papadakis
D
D
Alikaridis
F
F
Kephalas
T
T
Larsen
C
C
Palaiologos
G
G
D000596:Amino Acids / Q000302:isolation & purification / Q000378:metabolism; D000818:Animals; D002540:Cerebral Cortex / Q000378:metabolism*; D002849:Chromatography, Gas; D008401:Gas Chromatography-Mass Spectrometry; D066298:In Vitro Techniques; D007532:Isoleucine / Q000302:isolation & purification / Q000378:metabolism*; D007553:Isotope Labeling / Q000379:methods; D007930:Leucine / Q000378:metabolism*; D008297:Male; D009587:Nitrogen Isotopes; D051381:Rats; D017208:Rats, Wistar
D000596:Amino Acids; D009587:Nitrogen Isotopes; D007532:Isoleucine; D007930:Leucine