Immune complexes in blood: a new strategy for the analysis of their antigen portion.
A method is described for the characterization of protein antigens from circulating immune complexes from plasma. Free immunoglobulins G were separated from larger immune complexes by gel filtration with a fast protein liquid chromatographic system. The collected immune complexes were dissociated with 4M urea into antigens and antibodies. With a second column run with 4M urea, antigens smaller than 120 kDa were separated from unloaded antibody fractions. After concentration, they were analyzed by two-dimensional gel electrophoresis.
Fried
R
R
Institute of Clinical Chemistry, University Hospital Zürich, Switzerland.
Wiederkehr
F
F
Vonderschmitt
D J
DJ
D000936:Antigen-Antibody Complex / Q000097:blood*; D000941:Antigens / Q000097:blood*; D001798:Blood Proteins / Q000276:immunology; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D015180:Electrophoresis, Gel, Two-Dimensional; D005069:Evaluation Studies as Topic; D006801:Humans; D014508:Urea
D000936:Antigen-Antibody Complex; D000941:Antigens; D001798:Blood Proteins; D014508:Urea