title: Synthesis of a dibenz[b,e]oxepin-bovine serum albumin conjugate for radioimmunoassay of KW-4679 ((Z)-11-[3-(dimethylamino)propylidene]-6,11- dihydrodibenz[b,e]oxepin-2-acetic acid hydrochloride).
abstract: (Z)-11-[3-(Dimethylamino)propylidene]-2-(methoxycarbonyl)methyl-6, 11- dihydrodibenz[b,e]oxepin-9-acrylic acid (5) was prepared for application to the radioimmunoassay of KW-4679 (1, (Z)-11-[3-(dimethylamino)propylidene]-6,11-dihydrodibenz[b,e ] oxepin-2-acetic acid hydrochloride). The acrylic acid moiety in the 9-position of 5 was employed for coupling with an amino group of bovine serum albumin (BSA) to provide 17. Subsequently, the conjugate 17 was treated with aqueous NaOH to hydrolyze the terminal methoxycarbonyl group in the 2-position of the BSA conjugated 5. Antiserum raised against the antigenic BSA-conjugate 4 finally obtained was specific for 1.
authors.affiliation: Pharmaceutical Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., Shizuoka, Japan.
authors.forename: E
authors.initials: E
authors.lastname: Ohshima
authors.forename: H
authors.initials: H
authors.lastname: Sato
authors.forename: H
authors.initials: H
authors.lastname: Obase
authors.forename: T
authors.initials: T
authors.lastname: Uchimura
authors.forename: T
authors.initials: T
authors.lastname: Kuwabara
authors.forename: S
authors.initials: S
authors.lastname: Kobayashi
mesh_terms: D000941:Antigens / Q000737:chemistry; D003990:Dibenzoxepins / Q000276:immunology*; D000069605:Olopatadine Hydrochloride; D011863:Radioimmunoassay; D012710:Serum Albumin, Bovine / Q000276:immunology
chemical_list: D000941:Antigens; D003990:Dibenzoxepins; D012710:Serum Albumin, Bovine; D000069605:Olopatadine Hydrochloride