An immunocytochemical test for the diagnosis of antibodies to Plasmodium vivax.
An immunocytochemical peroxidase test (ICPT) has been developed to allow serological measurement of the antibody response to Plasmodium vivax by light microscopy. Acetone fixed P. vivax erythrocyte stages were used as source of antigen. The immunofluorescent antibody test (IFAT) was used as reference test. In testing sera from individuals infected with P. vivax in southeastern Venezuela a high correlation (100%) was obtained between the ICPT and the IFAT. There were cross reactions with sera from patients with malaria by P. falciparum but not with those from patients with other parasitic diseases. Antibody titres as measured by the ICPT showed a positive correlation with past P. vivax malarial experiences.
Bracho
C
C
Instituto Venezolano de Investigaciones Cientificas, Centro de Microbiologia y Biologia Celular, Caracas.
Perez
H
H
D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood*; D005069:Evaluation Studies as Topic; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods* / Q000706:statistics & numerical data; D007159:Immunologic Tests / Q000379:methods / Q000706:statistics & numerical data; D016780:Malaria, Vivax / Q000175:diagnosis* / Q000276:immunology*; D010966:Plasmodium vivax / Q000276:immunology*; D012680:Sensitivity and Specificity
D000913:Antibodies, Protozoan