Characterization of human immunodeficiency virus-1-infected cells of myeloid-monocytic lineage (ML-1, HL-60, THP-1, U-937).
The myeloid-monocytic cells ML-1, HL-60, THP-1, and U-937 were chronically infected (for > 2 years) with the lymphotropic human immunodeficiency virus type 1 (HIV-1) strain HTLV-IIIB. Reinfection experiments revealed that viruses obtained from chronically infected ML-1/HIV-1 and HL-60/HIV-1 cells showed a low infectivity if tested with uninfected ML-1 and HL-60 cells in contrast to virus preparations from chronically infected THP-1/HIV-1 and U-937/HIV-1 with their corresponding uninfected cell lines. Analyses of selected cell surface markers revealed a differential expression of CD4, CD8, CD11c, CD14, CD15, CD20, HLA-DR, and HLA-DQ in non- or chronically infected cells. In chronically infected cells, the steady-state levels for tumor necrosis factor-alpha, interleukin (IL)-1 beta, and granulocyte-macrophage colony-stimulating factor mRNA remained unchanged whereas the one for IL-6 dropped.
Ushijima
H
H
Division of AIDS Virus, AIDS Research Center, National Institute of Health, Tokyo, Japan.
Kunisada
T
T
Ami
Y
Y
Tsuchie
H
H
Takahashi
I
I
Klöcking
H P
HP
Müller
W E
WE
D015703:Antigens, CD / Q000032:analysis*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000235:genetics; D015497:HIV-1 / Q000235:genetics / Q000502:physiology*; D006683:HLA-DQ Antigens / Q000032:analysis; D006684:HLA-DR Antigens / Q000032:analysis; D006801:Humans; D007375:Interleukin-1 / Q000235:genetics; D015850:Interleukin-6 / Q000235:genetics; D015473:Leukemia, Promyelocytic, Acute; D009000:Monocytes; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics
D015703:Antigens, CD; D006683:HLA-DQ Antigens; D006684:HLA-DR Antigens; D007375:Interleukin-1; D015850:Interleukin-6; D012333:RNA, Messenger; D012367:RNA, Viral; D014409:Tumor Necrosis Factor-alpha; D016178:Granulocyte-Macrophage Colony-Stimulating Factor