Trichinella strain, pig race and other parasitic infections as factors in the reliability of ELISA for the detection of swine trichinellosis.
An enzyme-linked immunosorbent assay (ELISA) using crude worm extracts (CWE) and mixtures of these as antigens of five Spanish isolates (P, C, B1, B2 and W) was developed for detecting homologous and heterologous experimental infections with these isolates between-14 and 82 days post-infection (p.i.) in white and Iberian pigs. A total of 243 pigs (Iberian or cross-bred with this race) with numerous parasitic infections were also screened for the presence of antibodies to a mixture of CWE of C, B1 and B2 isolate. The test showed a specificity of 93.1-98.9% depending on the cut-off values and a maximum sensitivity of 92.8-100% between days 34 and 82 p.i. A low grade of infectivity was shown in the T3 isolates compared to the T1 isolates (P, C, B1 and B2) but high cross-reactions were observed between all the isolates with minor differences between P and W isolates. The highest antibody response was found in P infections and the lowest in pigs infected with the W isolate. A clear association between the presence of several parasitic infections and false positive reactions was not found, but an important relation was shown between high background levels and the Iberian race in experimentally and conventionally raised pigs.
Serrano
F
F
Department of Animal Health and Medicine, Faculty of Veterinary Sciences, University of Extremadura, Cáceres, Spain.
Pérez
E
E
Reina
D
D
Navarrete
I
I
D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D000947:Antigens, Helminth / Q000276:immunology; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D008297:Male; D012680:Sensitivity and Specificity; D013030:Spain; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis* / Q000469:parasitology; D014234:Trichinella / Q000145:classification / Q000276:immunology*; D014235:Trichinellosis / Q000175:diagnosis / Q000469:parasitology / Q000662:veterinary*
D000909:Antibodies, Helminth; D000947:Antigens, Helminth