Non-radioactive nucleic acid probes: labelling and detection procedures.
A comprehensive overview is given concerning the in situ hybridization techniques using non-radioactively labelled probes. Details are given on the preparation of the probes with different reporter molecules and on the corresponding revealing assays. The development of non-isotopic labelling methods for nucleic acid probes has facilitated the diffusion of the in situ hybridization techniques to the research as well as to the clinical pathology laboratory. Non-radioactive probes have several advantages over the radiolabelled ones, including cost, shelf-life, safety and, last but not least, resolution of the signal.
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Department of Pathology, University of Milan School of Medicine, Italy.
Dell'Orto
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D015073:2-Acetylaminofluorene; D001710:Biotin; D001973:Bromodeoxyuridine; D004076:Digoxigenin; D006241:Haptens; D007150:Immunohistochemistry; D017403:In Situ Hybridization / Q000379:methods*; D015345:Oligonucleotide Probes / Q000737:chemistry
D006241:Haptens; D015345:Oligonucleotide Probes; D001710:Biotin; D015073:2-Acetylaminofluorene; D001973:Bromodeoxyuridine; D004076:Digoxigenin