title: C3 fixed in vivo to cornea from horses inoculated with Leptospira interrogans.
abstract: C3 was detected bound in vivo to the opaque cornea of horses inoculated with killed Leptospira interrogans. Employing epithelial corneal cells isolated from a monolayer in tissue culture, we proved that C3 is fixed in vitro to the intact cell surface after incubation with a fresh equine anti-Leptospira serum. These findings, in addition to the infiltration of cornea with neutrophils and lymphocytes, may explain the mechanisms of tissue damage in recurrent uveitis of horses with leptospirosis.
authors.affiliation: Lab. Inmunoquimica y Biotecnologia, Facultad Cs. Veterinarias, Univ. Nac. del Centro PBA, Tandil, Argentina.
authors.forename: A E
authors.initials: AE
authors.lastname: Parma
authors.forename: S I
authors.initials: SI
authors.lastname: Cerone
authors.forename: S A
authors.initials: SA
authors.lastname: Sansinanea
authors.forename: M
authors.initials: M
authors.lastname: Ghezzi
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003176:Complement C3 / Q000276:immunology*; D003168:Complement Fixation Tests / Q000662:veterinary; D003315:Cornea / Q000276:immunology* / Q000382:microbiology; D003318:Corneal Opacity / Q000662:veterinary; D004848:Epithelium / Q000276:immunology / Q000382:microbiology; D015818:Eye Infections, Bacterial / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*; D005455:Fluorescent Antibody Technique / Q000662:veterinary; D006734:Horse Diseases / Q000276:immunology* / Q000382:microbiology; D006736:Horses; D007921:Leptospira interrogans / Q000276:immunology*; D007922:Leptospirosis / Q000276:immunology / Q000662:veterinary*; D008297:Male; D014605:Uveitis / Q000662:veterinary
chemical_list: D003176:Complement C3