Fos-like expression and nuclear size in osmotically stimulated supraoptic nucleus neurons.
This study has analysed by immunocytochemistry the pattern of expression of Fos-related proteins, as well as variations in nuclear size, after the osmotically induced activation of supraoptic nucleus neurons of the rat. In control rats most supraoptic nucleus neurons were Fos-like negative. After acute and chronic dehydration by salt-loading, the number of Fos-like positive neurons increased dramatically. The level of Fos-like immunoreactivity was higher in chronically stimulated rats, and also the neurons of the ventral region of the supraoptic nucleus were more intensely stained than those of the dorsal region. The karyometric analysis was made on electron micrographs. The mean nuclear profile area showed a significant increase in dehydrated rats with respect to the controls (73 +/- 16 microns 2 in those dehydrated for six days vs 54 +/- 13 in controls, mean +/- S.D.). However, no significant differences in this parameter were found when one-day and six-day dehydrated groups were compared. The invagination factor of the nuclear membrane, a nuclear shape indicator, decreased significantly in dehydrated rats, indicating a tendency towards spherical nuclei. It is noteworthy that the nuclear profile perimeter was constant, about 32 microns, in control and osmotically simulated rats. The higher nuclear accumulation of Fos-related antigens after six days of dehydration suggests that in chronically stimulated supraoptic nucleus neurons there is a sustained induction of cell-specific genes. Moreover, the transcription rate of the target genes containing the consensus DNA sequence TGAC/GTCA or c-AMP responsive elements recognition sites may depend upon the nuclear concentration of Fos-related antigens in supraoptic nucleus neurons. Our results also suggest that the initial Fos-related antigen expression and nuclear size increase are triggered concomitantly in supraoptic nucleus neurons after a short period of osmotic stimulation. On the other hand, we propose that nuclear envelope invaginations represent a reservoir of nuclear membrane which allows dynamic changes in nuclear size and shape depending on the metabolic status of the supraoptic nucleus neurons.
Lafarga
M
M
Departamento de Anatomía y Biologia Celular, Facultad de Medicina, Universidad de Cantabria, Santander, Spain.
Berciano
M T
MT
Martinez-Guijarro
F J
FJ
Andres
M A
MA
Mellström
B
B
Lopez-Garcia
C
C
Naranjo
J R
JR
D000818:Animals; D002467:Cell Nucleus / Q000648:ultrastructure*; D005786:Gene Expression Regulation / Q000502:physiology*; D016762:Genes, fos; D007032:Hypothalamus, Anterior / Q000648:ultrastructure*; D008297:Male; D009474:Neurons / Q000648:ultrastructure*; D009994:Osmolar Concentration; D051381:Rats; D017207:Rats, Sprague-Dawley