Rat erythrocyte glycophorins can be isolated by the lithium diiodosalicylate method used for other glycophorins.
1. The lithium diiodosalicylate/phenol method, widely employed for the isolation of membrane sialoglycoproteins (glycophorins) from mammalian erythrocytes, was applied for the first time to the purification of homologous glycoproteins from rat erythrocyte membranes. 2. The resulting preparations showed to be composed of four components, fractionated on SDS-PAGE. All four were positive for periodic acid-Schiff's reagent stain, the two largest of them being major. 3. Isolated rat glycophorins accounted for 60% of the ghost sialic acid and 1.5% of their protein. The presence of O-acetyl groups was confirmed in one-third of the sialic acid residues. 4. The molecular masses of the four glycophorin components were determined by a method which takes into account the anomalous mobility of glycoproteins on SDS-electrophoresis. Estimated values thus obtained for the actual molecular masses were 74, 32, 25 and 17 kDa.
Herráez
A
A
Departamento de Bioquímica y Biología Molecular, Universidad de Alcalá, Madrid, Spain.
Díez
J C
JC
Luque
J
J
D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D004910:Erythrocyte Membrane / Q000737:chemistry*; D006021:Glycophorins / Q000302:isolation & purification*; D006801:Humans; D007463:Iodobenzoates; D008094:Lithium; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012459:Salicylates; D012995:Solubility
D006021:Glycophorins; D007463:Iodobenzoates; D012459:Salicylates; C020891:3,5-diiodosalicylic acid; D008094:Lithium