title: Ergosterol levels in two L-methionine-enriched mutants of the methylotrophic yeast Candida boidinii ICCF26.
abstract: Two L-methionine-enriched mutants, SN-78 and SE-57, were isolated in a sulphur-deficient medium from the methylotrophic yeast Candida boidinii ICCF26. No significant differences were detected between the L-cysteine pools of the mutants and the wild-type. In mutant strain SE-57, S-adenosylmethionine and ergosterol levels were higher than in the wild-type strain, while in the other mutant, SN-78, the levels were lower. The evidence presented would suggest that, in both the mutants and the wild-type used in this study, S-adenosylmethionine was of key importance for the accumulation of L-methionine and ergosterol.
authors.affiliation: Department of Genetic Engineering, Institute of Biology, Bucharest, Romania.
authors.forename: D
authors.initials: D
authors.lastname: Avram
authors.forename: R
authors.initials: R
authors.lastname: Stan
mesh_terms: D002175:Candida / Q000235:genetics / Q000378:metabolism*; D003545:Cysteine / Q000378:metabolism; D004875:Ergosterol / Q000378:metabolism*; D008715:Methionine / Q000378:metabolism*; D016296:Mutagenesis; D012436:S-Adenosylmethionine / Q000378:metabolism
chemical_list: D012436:S-Adenosylmethionine; D008715:Methionine; D003545:Cysteine; D004875:Ergosterol