Evidence of the presence of GTPase inhibiting proteins for a low M(r) GTP-binding protein, ram p25, in rat spleen and pheochromocytoma PC12 cells.
1. GTPase inhibiting activity for a low M(r) GTP-binding protein, ram p25, was detected in the cytosolic fractions of rat spleen and PC12 cells. 2. The inhibitors were heat-labile and trypsin-sensitive, indicating that they were of proteinous nature. 3. The molecular mass of the inhibitor in the spleen appeared to be about 65 kDa on the elution profile from the gel filtration column.
Nagata
K
K
Department of Biochemistry, Gifu University School of Medicine, Japan.
Suzuki
T
T
Okano
Y
Y
Nozawa
Y
Y
D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D002850:Chromatography, Gel; D003600:Cytosol / Q000737:chemistry; D004355:Drug Stability; D020558:GTP Phosphohydrolases / Q000037:antagonists & inhibitors*; D019204:GTP-Binding Proteins / Q000037:antagonists & inhibitors*; D006358:Hot Temperature; D008970:Molecular Weight; D016716:PC12 Cells; D011506:Proteins / Q000032:analysis / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats; D013154:Spleen / Q000737:chemistry*; D014357:Trypsin / Q000494:pharmacology; D020691:rab GTP-Binding Proteins; D000075745:rab27 GTP-Binding Proteins
D011506:Proteins; D000075745:rab27 GTP-Binding Proteins; D000255:Adenosine Triphosphate; D014357:Trypsin; D020558:GTP Phosphohydrolases; D019204:GTP-Binding Proteins; C512961:Rab27a protein, rat; D020691:rab GTP-Binding Proteins