title: Identification of Leishmania from mucosal leishmaniasis by recombinant DNA probes.
abstract: Three cases of mucosal leishmaniasis are described. Parasites isolated from mucosal lesions were identified by Southern blot analysis of their genomic deoxyribonucleic acids (DNAs) using recombinant DNA probe pDK20. Parasites from 2 patients were identified as Leishmania donovani s.l. One of the patients had pure mucosal lesions, while in the second patient there was dissemination of the parasite to other organs. The spectrum of the disease caused by L. donovani is discussed. The parasite from the third patient was identified as L. major.
authors.affiliation: Department of Microbiology, College of Medicine, University of Fuba, Khartoum Centre, Sudan.
authors.forename: H W
authors.initials: HW
authors.lastname: Ghalib
authors.forename: E A
authors.initials: EA
authors.lastname: Eltoum
authors.forename: C C
authors.initials: CC
authors.lastname: Kroon
authors.forename: A M
authors.initials: AM
authors.lastname: el Hassan
mesh_terms: D000328:Adult; D000818:Animals; D015139:Blotting, Southern; D015342:DNA Probes; D006801:Humans; D007891:Leishmania / Q000235:genetics / Q000302:isolation & purification*; D007893:Leishmania donovani / Q000302:isolation & purification; D007896:Leishmaniasis / Q000469:parasitology*; D008297:Male; D008875:Middle Aged; D009300:Nasal Septum / Q000469:parasitology*; D009668:Nose Diseases / Q000469:parasitology; D013397:Sudan
chemical_list: D015342:DNA Probes