A simple protocol for the automation of DNA cycle sequencing reactions and polymerase chain reactions.
Automated DNA sequencing methods using robotic workstations have been previously reported, however it is often an arduous task to import these technologies into a laboratory. We describe protocols making use of a Beckman Biomek 1000 robotic workstation to prepare polymerase chain reactions (PCRs) and "cycle sequencing" reactions to be performed in a Perkin Elmer Cetus System 9600 thermocycler. The combination of these two instruments allows a high throughput of PCR and DNA sequencing reactions. The programs described are freely available via anonymous file transfer protocol (FTP).
Civitello
A B
AB
Institute for Molecular Genetics, Baylor College of Medicine, Houston, Texas 77030.
Richards
S
S
Gibbs
R A
RA
D017104:Bacteriophage M13 / Q000235:genetics; D001483:Base Sequence; D004279:DNA, Viral / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012371:Robotics; D017422:Sequence Analysis, DNA / Q000295:instrumentation*
D004279:DNA, Viral