Endogenous superoxide dismutase activity in two Babesia species.
Babesia hylomysci and B. divergens were studied for superoxide dismutase (SOD) activity by enzyme assay and isoelectric focusing (IEF). In the two Babesia species, parasite-associated SOD is cyanide-insensitive and inhibited by H2O2, indicating that iron is the cofactor metal. Measurements of SOD activity from purified parasites show that the SOD activity detected in Babesia is, for the main part, due to an endogenous enzyme.
Becuwe
P
P
INSERM U42, Villeneuve d'Ascq, France.
Slomianny
C
C
Valentin
A
A
Schrevel
J
J
Camus
D
D
Dive
D
D
D000818:Animals; D001403:Babesia / Q000201:enzymology* / Q000648:ultrastructure; D002478:Cells, Cultured; D004912:Erythrocytes / Q000469:parasitology; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D007525:Isoelectric Focusing; D051379:Mice; D008854:Microscopy, Electron; D011190:Potassium Cyanide / Q000494:pharmacology; D013482:Superoxide Dismutase / Q000032:analysis* / Q000037:antagonists & inhibitors
D006861:Hydrogen Peroxide; D013482:Superoxide Dismutase; D011190:Potassium Cyanide