A cDNA clone with anti-oncogene activity.
A cDNA clone, p14-6, which has an anti-oncogene activity on the v-Ki-Ras oncogene-transformed malignant cell line DT, was found. This clone was recovered from the revertant R14 cells, which had been isolated by transfections of DT cells with a normal human fibroblast cDNA library cloned in pcD2, an Okayama-Berg vector. When transfected into DT cells, p14-6 clone gave rise to phenotypical flat reversion in 5-15% of DT transfectant colonies. The p14-6-transfected flat cell line, RR, was proven to be a true revertant with significantly reduced malignancy by in vitro and in vivo malignancy tests. All other clones recovered from R14 cells were unable to cause this reversion. Molecular hybridizations showed that the p14-6 was inserted into RR genome as tandem repeats, and no structural change was found in the v-Ki-Ras oncogene in RR genome. These facts suggest that the anti-oncogene activity of the p14-6 clone on the DT cells may be exerted through expression of the cDNA contained in this clone.
Liu
D G
DG
Shanghai Institute of Biochemistry, Academia Sinica, PRC.
Wang
D
D
Chen
Z Z
ZZ
Li
Z P
ZP
Makoto
N
N
Yoji
I
I
D000818:Animals; D002459:Cell Fusion; D003001:Cloning, Molecular; D005347:Fibroblasts; D016147:Genes, Tumor Suppressor / Q000235:genetics*; D011905:Genes, ras / Q000235:genetics*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D010957:Plasmids; D014162:Transfection