Western blotting analysis in patients with MS using human brain vessels as antigen.
Serum samples from patients suffering from multiple sclerosis, other neurological diseases and normal controls were screened by "western blotting" for antibody directed against proteins of human brain vessels purified from a post mortem brain. A small number of sera contained autoantibodies against some of the proteins of the brain vessels, particularly in patients suffering from MS, epilepsy and migraine. The significance of these results is discussed.
Souberbielle
B E
BE
Department of Biochemistry, University of St Andrews, Scotland.
Swingler
R J
RJ
Davidson
D L
DL
Cull
R E
RE
Atkinson
S
S
Davison
I
I
Anderson
J
J
Bell
J E
JE
Russell
W C
WC
D000918:Antibody Specificity / Q000276:immunology; D001323:Autoantibodies / Q000032:analysis*; D001812:Blood-Brain Barrier / Q000276:immunology; D015153:Blotting, Western; D001921:Brain / Q000098:blood supply*; D004730:Endothelium, Vascular / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D008855:Microscopy, Electron, Scanning; D009103:Multiple Sclerosis / Q000276:immunology*; D009131:Muscle, Smooth, Vascular / Q000276:immunology*; D009422:Nervous System Diseases / Q000276:immunology
D001323:Autoantibodies; D007074:Immunoglobulin G