Concentration of metronidazole in periodontal pockets after application of a metronidazole 25% dental gel.
The metronidazole concentration was monitored in gingival crevicular fluid (GCF) of inflamed periodontal pockets after 1 application of a 25% gel. 12 patients with periodontal disease were included in the study. Metronidazole gel was applied with a syringe into the pockets of 10 teeth with probing depths > or = 5 mm. Samples of GCF were taken with Periopaper before application and 4, 8, 12, 24 and 36 h after application. At each time, samples from 2 teeth were pooled in order to obtain a sufficient amount of fluid for analysis. By means of a calibrated Periotron, the collected volume of GCF was measured. High-performance liquid chromatography was used to determine the amount of metronidazole. MIC50 for anaerobic periopathogens susceptible to metronidazole is below 1 microgram/ml. In this study, the concentration obtained was higher than 1 microgram/ml in all samples after 4 and 8 h, in 92% after 12 h, in 50% after 24 h and in 8% after 36 h. Thus, metronidazole concentrations in the pockets were generally above MIC50 for susceptible periopathogens 24 h after 1 application of a 25% metronidazole gel.
Stoltze
K
K
Department of Periodontology, School of Dentistry, Faculty of Health Sciences, University of Copenhagen, Denmark.
D000328:Adult; D002851:Chromatography, High Pressure Liquid; D003692:Delayed-Action Preparations; D004343:Drug Implants; D005260:Female; D005782:Gels; D005883:Gingival Crevicular Fluid / Q000737:chemistry*; D006801:Humans; D008297:Male; D008795:Metronidazole / Q000008:administration & dosage / Q000032:analysis* / Q000627:therapeutic use*; D008875:Middle Aged; D010514:Periodontal Pocket / Q000188:drug therapy* / Q000378:metabolism*; D013997:Time Factors
D003692:Delayed-Action Preparations; D004343:Drug Implants; D005782:Gels; D008795:Metronidazole