[The mechanism and specificity of IL-1 inhibitory factor released from human alveolar macrophages].
We have previously reported the presence of interleukin 1 (IL-1) inhibitory factor in the culture supernatants of alveolar macrophages. The activity is decreased in healthy smokers and patients with interstitial lung diseases (sarcoidosis, idiopathic pulmonary fibrosis), compared with healthy non-smokers. In this study, we further examined the mechanism and specificity of IL-1 inhibitory factor. The inhibitory factor exhibited specific inhibition of augmentation by IL-1 of PHA induced murine thymocyte proliferation, whereas there was no inhibition of the augmentation by IL-2, IL-4, IL-6 or TNF. This IL-1 inhibitory factor competitively blocked the binding of IL-1 to the IL-1 receptor on PHA-stimulated murine thymocytes. These results indicate that alveolar macrophages produce a specific IL-1 inhibitory factor which functions as a IL-1 receptor antagonist.
Takeuchi
M
M
Second Department of Medicine, Kyoto University, Japan.
Aung
H
H
Nagai
S
S
D000328:Adult; D000818:Animals; D001667:Binding, Competitive; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D006801:Humans; D007375:Interleukin-1 / Q000037:antagonists & inhibitors* / Q000378:metabolism; D008222:Lymphokines / Q000378:metabolism / Q000494:pharmacology* / Q000502:physiology; D016676:Macrophages, Alveolar / Q000378:metabolism*; D051379:Mice; D008875:Middle Aged; D017472:Receptors, Interleukin-1 / Q000378:metabolism; D013950:Thymus Gland / Q000166:cytology
D007375:Interleukin-1; D008222:Lymphokines; D017472:Receptors, Interleukin-1