Noise, sensitivity, and resolution of flow cytometers.
The sensitivity and resolution of flow cytometers are functions of the signal produced by a given particle as well as by the noise in the presence of which the signal is detected. The noise is primarily due to the fact that emission of light as well as its detection by photoelectric devises are stochastic processes. This fact leads to equations describing how resolution and sensitivity are limited by the magnitude of the signal, the background, and the photoelectron quantum yield of the detector. The equations are pointing to a method by which the signal and noise of a flow cytometer can be measured in absolute terms, as well as a way to determine fluorescence sensitivity without having to extrapolate to the noise level. The equations appear to be validated when applied to measuring data obtained with two different flow cytometers.
Steen
H B
HB
Department of Biophysics, Norwegian Radium Hospital, Oslo.
D002138:Calibration; D005434:Flow Cytometry / Q000295:instrumentation; D005453:Fluorescence; D008433:Mathematics; D016012:Poisson Distribution; D012680:Sensitivity and Specificity; D013269:Stochastic Processes