Chloroplast tRNA(Asp): nucleotide sequence and variation of in vivo levels during plastid maturation.
Two chloroplast tRNA(Asp) species from barely were purified by chromatography on benzoylated DEAE-cellulose and sequenced. They differ in the modification at position 34, where queuosine (Q) is present in one of the species. The same chromatographic procedure yielded only one tRNA(Glu) species, corroborating the assumption that the same tRNA(Glu) species participates in both protein and chlorophyll biosynthesis. The level of tRNA(Glu) remains unchanged after light treatment of etiolated seedlings, whereas the amount of tRNA(Asp) decreases to about 50% relative to the level of dark-grown plants.
Schön
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Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06511.
Gough
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Söll
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D001483:Base Sequence; D015152:Blotting, Northern; D002482:Cellulose; D002736:Chloroplasts / Q000378:metabolism*; D002845:Chromatography / Q000379:methods; D014644:Genetic Variation; D001467:Hordeum / Q000235:genetics* / Q000254:growth & development / Q000528:radiation effects; D008027:Light; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012349:RNA, Transfer, Asp / Q000235:genetics*; D012352:RNA, Transfer, Glu / Q000235:genetics
D012349:RNA, Transfer, Asp; D012352:RNA, Transfer, Glu; D002482:Cellulose