Rapid beta-lactoglobulin genotyping of cattle using the polymerase chain reaction.
A polymerase chain reaction (PCR) assay has been developed for genotyping beta-lactoglobulin A and B variants in dairy cattle. Either blood or semen samples can be used as a source of DNA. The method is accurate, faster than Southern blot analyses and should prove a useful tool in breeding programmes.
Wilkins
R J
RJ
Ruakura Agricultural Centre, Hamilton, New Zealand.
Kuys
Y M
YM
D000818:Animals; D001483:Base Sequence; D002417:Cattle / Q000235:genetics*; D004247:DNA; D015252:Deoxyribonucleases, Type II Site-Specific; D005838:Genotype; D007782:Lactoglobulins / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*
D007782:Lactoglobulins; D004247:DNA; D015252:Deoxyribonucleases, Type II Site-Specific; C024412:GGTGA-specific type II deoxyribonucleases