title: Analysis of genomic DNA from Chlamydia trachomatis for Dam and Dcm methylation.
abstract: Chlamydia trachomatis is a Gram-negative eubacterium with a dimorphic developmental cycle and obligate intracellular growth in the eucaryotic host. The Dam transmethylase of Escherichia coli methylates at the N6 position of adenine in the sequence 5'-GATC-3' and the Dcm transmethylase adds methyl groups to the C5 position of the internal cytosines in the sequences 5'-CCWGG-3'. In contrast to E. coli, C. trachomatis DNA appears to have unmethylated Dam sites and only low level Dcm methylation.
authors.affiliation: Department of Pathology and Laboratory Medicine, University of California, Los Angeles.
authors.forename: E A
authors.initials: EA
authors.lastname: Wagar
authors.forename: S
authors.initials: S
authors.lastname: Safarians
authors.forename: M
authors.initials: M
authors.lastname: Pang
mesh_terms: D000225:Adenine / Q000737:chemistry; D001483:Base Sequence; D002692:Chlamydia trachomatis / Q000737:chemistry* / Q000201:enzymology / Q000235:genetics; D003596:Cytosine / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry* / Q000235:genetics; D015257:DNA-Cytosine Methylases / Q000378:metabolism; D008745:Methylation; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D015183:Restriction Mapping; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific) / Q000378:metabolism
chemical_list: D004269:DNA, Bacterial; D003596:Cytosine; D015257:DNA-Cytosine Methylases; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific); D000225:Adenine