title: Secretory proteins and glycoconjugates synthesized by human tracheal gland cells in culture.
abstract: To study the proteins and glycoconjugates synthesized by serous cells from human tracheal glands (HTG), isolated HTG cells were cultured in the presence of radiolabeled precursors 14C-proline, Na2(35)SO4, and 3H-fucose. The secretory 14C/35S/3H-radiolabeled proteins and glycoproteins, de novo synthesized by HTG cells, were analyzed by gel filtration chromatography. We observed the incorporation of 14C-proline into antileukoprotease and an unknown 30 kD protein, and the incorporation of 35SO4-- and 3H-fucose into high molecular weight glycoconjugates and sulfoconjugates (M(r) > 1,000,000) and into components with apparent M(r) of approximately 250 and 100 kD. After specific chemical and enzymatic treatment, the 35S- and 3H-glycoconjugates were shown to be -O-linked mucin-like glycoproteins and proteoglycans. These results show that cultured HTG cells synthesize some of the macromolecules identified in bronchial secretions.
authors.affiliation: INSERM U 14, Plateau de Brabois, Vandoeuvre lès Nancy, France.
authors.forename: M D
authors.initials: MD
authors.lastname: Merten
authors.forename: J M
authors.initials: JM
authors.lastname: Tournier
authors.forename: Y
authors.initials: Y
authors.lastname: Meckler
authors.forename: C
authors.initials: C
authors.lastname: Figarella
mesh_terms: D002478:Cells, Cultured; D002850:Chromatography, Gel; D005088:Exocrine Glands / Q000378:metabolism; D006001:Glycoconjugates / Q000096:biosynthesis*; D006801:Humans; D007700:Kinetics; D014176:Protein Biosynthesis; D014132:Trachea / Q000166:cytology / Q000378:metabolism*
chemical_list: D006001:Glycoconjugates