Escherichia coli B lacks one of the two initiator tRNA species present in E. coli K-12.
We show that the metY locus which specifies tRNA(2fMet) in Escherichia coli K-12 specifies tRNA(1fMet) in E. coli B. This conclusion is based on results of Southern blot analysis of E. coli B and K-12 DNAs and on polymerase chain reaction amplification, cloning, and sequencing of an approximately 200-bp region of DNA corresponding to the metY loci of E. coli B and E. coli K-12. We also show that the metY locus of E. coli B is transcriptionally active. E. coli strains transformed with the multicopy plasmid vector pUC19 carrying the metY locus of E. coli B overproduce tRNA(1fMet) in E. coli B and E. coli K-12 in contrast to strains transformed with pUC19 carrying the corresponding locus from E. coli K-12, which overproduce tRNA(2fMet).
Mandal
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Department of Biology, Massachusetts Institute of Technology, Cambridge 02139.
RajBhandary
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D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D009239:N-Formylmethionine / Q000378:metabolism*; D009690:Nucleic Acid Conformation; D016133:Polymerase Chain Reaction; D012358:RNA, Transfer, Met / Q000032:analysis / Q000235:genetics*; D014158:Transcription, Genetic
D012358:RNA, Transfer, Met; D009239:N-Formylmethionine