Cloning and expression of the aspartate carbamoyltransferase gene from Treponema denticola.
Treponema denticola seems to play a central role in the etiology of human periodontal disease. We have cloned an antigenic protein-coding sequence from T. denticola ATCC 33520. The protein-coding region was found to be a 3-kbp HindIII-HindIII fragment. The open reading frame consists of 1,426 bp and codes for a protein with an M(r) of 54,919. The deduced amino acid sequence showed 33.8% homology with that of the aspartate carbamoyltransferase of Escherichia coli. The gene products showed aspartate carbamoyltransferase activity.
Ishihara
K
K
Department of Microbiology, Tokyo Dental College, Chiba, Japan.
Ishihara
M
M
Takazoe
I
I
Okuda
K
K
D000595:Amino Acid Sequence; D001221:Aspartate Carbamoyltransferase / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D014208:Treponema / Q000201:enzymology / Q000235:genetics*
D001221:Aspartate Carbamoyltransferase