Use of 6 M HCl for removal of the N-alpha-tert-butyloxycarbonyl group during solid-phase peptide synthesis.
We describe the use of 6 M HCl to remove the Boc group in solid-phase peptide synthesis. Three peptides, 4-10 amino acid residues in length, have been successfully synthesized using 6 M HCl and were found to be comparable in purity to the same peptides prepared using the standard 50% trifluoroacetic acid in dichloromethane method of N-alpha-Boc removal.
Naharissoa
H
H
Centre de Recherches de Biochimie Macromoleculaire, CNRS UPR 9008, France.
Sarrade
V
V
Follet
M
M
Calas
B
B
D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid; D005563:Formic Acid Esters / Q000737:chemistry*; D006851:Hydrochloric Acid; D008722:Methods; D008903:Minerals / Q000737:chemistry; D008969:Molecular Sequence Data; D015394:Molecular Structure; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry; D012996:Solutions; D013464:Sulfuric Acids / Q000737:chemistry
D005563:Formic Acid Esters; D008903:Minerals; D010455:Peptides; D012996:Solutions; D013464:Sulfuric Acids; C000345:t-butyloxycarbonyl group; C033158:sulfuric acid; D006851:Hydrochloric Acid