A comparative analysis of two preparations of human chorionic gonadotrophin.
A biophysical analysis of two pharmaceutical preparations of human chorionic gonadotrophin (Endo and Profasi) demonstrated that both contained high hCG specific activity. HCG protein analysis by occlusion HPLC and SDS gel electrophoresis showed similar profiles for both products. Although the purified protein components of both preparations were similar their were differences in the contents of the unpurified preparations. The Endo preparation exhibited greater inter-vial variability and contained an unidentified aromatic substance which interacted with the desalting column.
Forman
R G
RG
Nuffield Department of Obstetrics and Gynaecology, University of Oxford, John Radcliffe Hospital, Headington, Oxford, UK.
Marshall
J
J
Robinson
J
J
Williams
S A
SA
D055598:Chemical Phenomena; D002627:Chemistry, Physical; D006063:Chorionic Gonadotropin / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans
D006063:Chorionic Gonadotropin