title: Control of gene expression in the temperate coliphage 186. X. The cI repressor directly represses transcription of the late control gene B.
abstract: We have found that the repressor of 186 lytic transcription, CI, represses transcription of the late control gene B, with no involvement of the B protein itself. In clone studies we showed that CI repressed transcription from the B promoter and that temperature inactivation of CIts led to B derepression. We conclude that CI repressor directly represses transcription of the B gene and, with prophage induction, it is probable that the inactivation of the CI repressor not only derepresses early lytic transcription, but also derepresses B gene transcription, leading to the activation of transcription from the late promoters.
authors.affiliation: Department of Biochemistry, University of Adelaide, South Australia.
authors.forename: J A
authors.initials: JA
authors.lastname: Dibbens
authors.forename: S L
authors.initials: SL
authors.lastname: Gregory
authors.forename: J B
authors.initials: JB
authors.lastname: Egan
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003090:Coliphages / Q000235:genetics* / Q000378:metabolism; D015967:Gene Expression Regulation, Viral / Q000187:drug effects; D005814:Genes, Viral; D008969:Molecular Sequence Data; D012097:Repressor Proteins / Q000502:physiology*; D014157:Transcription Factors / Q000096:biosynthesis* / Q000235:genetics; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*; D014775:Virus Activation
chemical_list: D012097:Repressor Proteins; D014157:Transcription Factors; D014764:Viral Proteins