[Microencapsulation in Na-alginate and in vitro development of sheep blastomeres].
Embryos at 4 cell stage obtained from Sarda ewes superovulated with FSHp (Sigma) were micromanipulated in order to obtain single blastomeres (1/4 E). The 1/4 E have been located randomly in two groups. In the first (Group A n. 30) the 1/4 E have been put back in empty zonae pellucidae; in the second (Group B n. 21) they have been microencapsulated in sodium alginate (1.1%) by dropping cell-alginate solution in a 1.5% CaCl2. Each capsule (1 mm diameter) contained four 1/4 E. The blastomeres have been co-cultured for 5 days in CZB medium on oviductal cell monolayer in a humidified incubator (5% CO2, 95% air, 38.5 degrees C). No differences were found between the groups reaching blastocyst stage after the end of the culture period (A 50%-B 47%).
Loi
P
P
Istituto Zootecnico e Caseario per la Sardegna, Olmedo, Sassari.
Ledda
S
S
Gallus
M
M
Filia
F
F
Cappai
P
P
Naitana
S
S
D000464:Alginates; D000818:Animals; D001757:Blastomeres / Q000502:physiology*; D046508:Culture Techniques / Q000379:methods*; D004339:Drug Compounding; D005314:Embryonic and Fetal Development / Q000502:physiology*; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D008846:Micromanipulation; D012756:Sheep / Q000196:embryology*; D015044:Zona Pellucida / Q000502:physiology*
D000464:Alginates; D006603:Hexuronic Acids; D020723:Glucuronic Acid
Microincapsulazione in Na-alginato e sviluppo in vitro di blastomeri di ovino.