Determination of methacrylonitrile in serum using gas chromatography and flame ionization detection.
A gas chromatographic method is described for the quantitation of methacrylonitrile in serum. Methacrylonitrile was extracted from rat serum with diethyl ether and then quantified using a gas chromatograph equipped with a flame ionization detector and a 60-m megabore column coated with polyethylene glycol polymer. The recoveries obtained following a one-step extraction with diethyl ether varied from 60% at 3.2 micrograms/ml to 70% at 80 micrograms/ml. The coefficient of variation for the analysis ranged from 2.5% at 400 micrograms/ml to 15.0% at 3.2 micrograms/ml.
Demby
K B
KB
National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709.
White
T C
TC
Ghanayem
B I
BI
D000818:Animals; D002849:Chromatography, Gas / Q000379:methods*; D005410:Flame Ionization / Q000379:methods*; D008689:Methacrylates / Q000032:analysis*; D009570:Nitriles / Q000097:blood*; D051381:Rats
D008689:Methacrylates; D009570:Nitriles; C008385:methacrylonitrile