Rederivation of rat colonies seropositive for Bacillus piliformis and the subsequent screening for antibodies.
Latent infection of rats in a breeding colony with Bacillus piliformis detectable by antibodies to the agent in an immunofluorescence assay was eliminated by a combination of traditional rederivation techniques, using animal units not previously used for rat breeding, and the use of specific disinfection procedures. The success rate was apparently correlated with the use of peracetic acid instead of aldehyde products to decontaminate the animal unit.
Hansen
A K
AK
Møllegaard Breeding Centre, Skensved, Denmark.
Skovgaard-Jensen
H J
HJ
Thomsen
P
P
Svendsen
O
O
Dagnaes-Hansen
F
F
Møllegaard-Hansen
K E
KE
D000822:Animal Husbandry; D000818:Animals; D000830:Animals, Laboratory / Q000276:immunology* / Q000382:microbiology*; D000907:Antibodies, Bacterial / Q000032:analysis*; D001407:Bacillus / Q000276:immunology*; D004203:Disinfection; D005455:Fluorescent Antibody Technique / Q000662:veterinary; D005856:Germ-Free Life; D006799:Housing, Animal; D051381:Rats / Q000276:immunology* / Q000382:microbiology*; D011919:Rats, Inbred Strains / Q000276:immunology / Q000382:microbiology; D013045:Species Specificity
D000907:Antibodies, Bacterial