Cell division arrest induced by phorbol ester in CHO cells overexpressing protein kinase C-delta subspecies.
Several lines of CHO cells stably overexpressing protein kinase C (PKC) subspecies to various extents were established by the DNA-mediated transfer. Upon treatment with phorbol 12-myristate 13-acetate, the growth of the cells expressing the PKC-delta subspecies was markedly inhibited, whereas cell lines expressing PKC-alpha, PKC-beta II, and PKC-zeta subspecies were not significantly affected. Flow cytometric analysis indicated that all cell lines overexpressing PKC-delta subspecies accumulated in G2/M phase in response to phorbol 12-myristate 13-acetate. In these arrested cells, dikaryons were predominant, implying that phorbol ester-induced inhibition of cell division is specific to telophase. These results suggest PKC-delta subspecies may play a role in the normal cell cycle progression.
Watanabe
T
T
Biology Laboratories, Research and Development Division, Takeda Chemical Industries, Osaka, Japan.
Ono
Y
Y
Taniyama
Y
Y
Hazama
K
K
Igarashi
K
K
Ogita
K
K
Kikkawa
U
U
Nishizuka
Y
Y
D000818:Animals; D016466:CHO Cells; D002453:Cell Cycle / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects*; D006224:Cricetinae; D004247:DNA / Q000235:genetics; D005784:Gene Amplification; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism*; D007700:Kinetics; D015240:Phorbol 12,13-Dibutyrate / Q000378:metabolism; D011485:Protein Binding; D011493:Protein Kinase C / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D014162:Transfection
D007527:Isoenzymes; D015240:Phorbol 12,13-Dibutyrate; D004247:DNA; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate