Production of monoclonal antibody in hollow fiber culture system with serum-free medium.
Hybridoma cells 7E8, secreting mouse monoclonal antibody (McAb) IgG against potato virus X, were cultured in hollow fiber cell culture system (module VF-2) with serum-free medium 1640SFM. The maximum viable cell density was found to be 2.34 x 10(6) cells/ml that was 3.7 and 2.2 times as high as those achieved in spinner and static flask culture respectively. About 10,000ml of cell culture media had been collected in the cultural period of 42 days. The ELISA titer of the McAb in the media was about 1:20,000 that was 25 times as high as those attained from the cultures in either spinner or static flask. From 1,000ml of cell culture media, an amount of 51.2mg of 7E8 McAb IgG had been purified by 50% saturated ammonium sulfate precipitation and Sephadex G-200 chromatography. The average yield of McAb in the hollow fiber cell culture system was 12.3mg/day. The results of the study suggest that it is hopeful for hybridoma cell culture in the hollow fiber cell culture system with serum-free medium for McAb production on large-scale.
Zhou
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Biotechnology Research Center, Chinese Academy of Agricultural Sciences, Beijing.
Cai
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S
D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000302:isolation & purification; D002452:Cell Count; D016895:Culture Media, Serum-Free; D004797:Enzyme-Linked Immunosorbent Assay; D006825:Hybridomas / Q000166:cytology*
D000911:Antibodies, Monoclonal; D016895:Culture Media, Serum-Free