[Antioxidants, lipid peroxidation, and receptor-dependent increase in Ca2+ concentration in human platelets].
Content of tocopherol and total antiradical activity of hydrophobic antioxidants were estimated in human blood platelets. Two hydrophobic antioxidants--tocopherol and ubiquinone were detected in hexane extracts of thrombocytes using thin-layer chromatography. After incubation of thrombocytes with N-ethyl maleimide content of malonyl dialdehyde was increased and of tocopherol--decreased, receptor-dependent increase of Ca2+ concentration in cytoplasm was potentiated, while the Ca(2+)-blocking effect of nitroglycerol was decreased. Nitroglycerol did not inhibit the N-ethyl maleimide effect on content of malonyl dialdehyde in blood platelets. Addition of exogenous vitamin E to thrombocytes did not affect the receptor-dependent increase of Ca2+ concentration. Ascorbic acid inhibited the receptor-dependent increase of Ca2+ concentration. The data obtained suggest that nitrates may be used more rationally in combination with antioxidants and/or inhibitors of cyclooxygenase.
Negresku
E V
EV
Lebedev
A V
AV
Baldenkov
G N
GN
Mazaev
A V
AV
Popovich
M I
MI
D000975:Antioxidants / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000494:pharmacology; D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism*; D002118:Calcium / Q000097:blood*; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D002855:Chromatography, Thin Layer; D005033:Ethylmaleimide / Q000494:pharmacology; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects*; D008315:Malondialdehyde / Q000378:metabolism; D005996:Nitroglycerin / Q000494:pharmacology; D014451:Ubiquinone / Q000378:metabolism; D014810:Vitamin E / Q000378:metabolism / Q000494:pharmacology
D000975:Antioxidants; D014451:Ubiquinone; D014810:Vitamin E; D008315:Malondialdehyde; D005996:Nitroglycerin; D005033:Ethylmaleimide; D001205:Ascorbic Acid; D002118:Calcium
Antioksidanty, perekisnoe okislenie lipidov i retseptorzavisimoe uvelichenie kontsentratsii Ca2+ v trombotsitakh cheloveka.