[Development of automated lactate analyzer with continuous blood sampling for monitoring blood lactate and its application for the testing of physical exercise].
The anaerobic threshold is useful for estimating the intensity of physical exercise. It is shown as either an increase in blood concentrations of lactate or a disproportionate increase in ventilation. We developed a lactate analyzer based on an electroenzymatic method with a continuous blood sampling system through a double-lumen catheter. Ascorbic acid, bilirubin, hemoglobin, creatinine, uric acid, and glucose did not interfere the results. The lactate concentrations in blood samples from healthy subjects during physical exercise correlated well (r = 0.993) with results measured by the conventional enzymatic method. We measured the concentrations of blood lactate with a use of this lactate analyzer to see the anaerobic threshold in nine healthy volunteers during exercise on a treadmill with an increasing workload. The point at which lactate concentrations started to increase was detected easily. The anaerobic threshold identified as a disproportionate increase in ventilation was seen at almost the same time. We conclude that the lactate analyzer, with a continuous blood sampling system, can measure precisely concentrations of lactate in blood and can detect the anaerobic threshold during physical exercise.
Fujino
K
K
Central Clinical Laboratory, Osaka City University Medical School.
Kitahashi
S
S
Nakao
M
M
Shimojo
N
N
Okuda
K
K
D000328:Adult; D015308:Anaerobic Threshold; D005080:Exercise Test / Q000295:instrumentation*; D006801:Humans; D007773:Lactates / Q000097:blood*; D008297:Male; D008991:Monitoring, Physiologic / Q000295:instrumentation*
D007773:Lactates