Determination of beta 2-microglobulin in serum by a microparticle-enhanced nephelometric immunoassay.
This fully automated nephelometric immunoassay to quantify beta 2-microglobulin in human serum measures the light-scattering signal produced by agglutination of commercially available latex microparticles (diameter 0.1 micron) coated with specific F(ab')2 against beta 2-microglobulin. The calibration curve, generated by serial dilutions of a beta 2-microglobulin standard of known concentration, is used to calculate beta 2-microglobulin concentrations in serum samples by the logit-log function and linear-regression analysis. The assay range (sample dilution 400-fold) extends from 0.3 to 40.0 mg/L. No antigen excess appears at beta 2-microglobulin concentrations up to 320 mg/L. Within-run CVs ranged from 1.0% to 3.4%, and between-days from 1.2% to 2.8%. Total imprecision (CV) was < 5%. Analytical recovery averaged 99.5% +/- 2.8%. Rheumatoid factor, complement, bilirubin (up to 340 mumol/L), and hemoglobin (up to 2.0 g/L) do not interfere. Strongly turbid lipemic samples must be cleared before analysis. Standard curve linearity was very good in samples covering the clinical useful range of concentrations. Results of the method correlated well with those of radioimmunoassay and microparticle enzyme-linked immunoassay (r = 0.979 and 0.975, respectively). The reference interval (nonparametric estimation) in apparently healthy adults (n = 303) was 0.87 (0.80-0.94) to 2.42 (2.28-2.45) mg/L; the median value was 1.54 mg/L.
Viedma
J A
JA
Department of Clinical Chemistry, Elche General Hospital, Alicante, Spain.
Pacheco
S
S
Albaladejo
M D
MD
D000328:Adult; D001322:Autoanalysis; D005260:Female; D006801:Humans; D007118:Immunoassay / Q000592:standards / Q000706:statistics & numerical data; D008055:Lipids / Q000097:blood; D008297:Male; D008875:Middle Aged; D009391:Nephelometry and Turbidimetry / Q000592:standards / Q000706:statistics & numerical data; D011786:Quality Control; D012016:Reference Values; D012044:Regression Analysis; D001613:beta 2-Microglobulin / Q000032:analysis*
D008055:Lipids; D001613:beta 2-Microglobulin