title: Mass spectrometric analysis of blotted proteins after gel electrophoretic separation by matrix-assisted laser desorption/ionization.
abstract: The molecular masses of electroblotted proteins were determined with a time-of-flight mass spectrometer by matrix-assisted laser desorption/ionization directly from blot membranes. Therefore standard proteins, separated by polyacrylamide gel electrophoresis, were electroblotted onto polyvinylidene difluoride or polyamide membranes by standard procedures. Pieces of membrane containing the protein of interest were soaked in matrix solution and analyzed in the mass spectrometer.
authors.affiliation: Max-Planck Institut für Biochemie, Martinsried, Germany.
authors.forename: C
authors.initials: C
authors.lastname: Eckerskorn
authors.forename: K
authors.initials: K
authors.lastname: Strupat
authors.forename: M
authors.initials: M
authors.lastname: Karas
authors.forename: F
authors.initials: F
authors.lastname: Hillenkamp
authors.forename: F
authors.initials: F
authors.lastname: Lottspeich
mesh_terms: D000327:Adsorption; D000390:Air Ionization; D000818:Animals; D002417:Cattle; D004591:Electrophoresis, Polyacrylamide Gel; D007834:Lasers; D013058:Mass Spectrometry / Q000379:methods*; D011506:Proteins / Q000737:chemistry* / Q000302:isolation & purification; D013055:Spectrophotometry, Infrared
chemical_list: D011506:Proteins