title: Bronchial hyperreactivity after inhalation of trimellitic anhydride dust in guinea pigs after intradermal sensitization to the free hapten.
abstract: We have developed in the guinea pig, an animal model of bronchial hyperreactivity provoked by inhalation of trimellitic anhydride (TMA) dust, a known cause of occupational asthma in humans, after intradermal sensitization to the free hapten. Male Dunkin-Hartley guinea pigs (n = 6) were injected intradermally with 0.1 ml of 30% TMA in corn oil. Control animals (n = 7) were injected with 0.1 ml corn oil alone. On Days 21 to 28 after sensitization, guinea pigs were challenged (nose only) to 12 mg/m3 of inhalable TMA dust for 30 min. Bronchial reactivity was measured in sensitized animals and in control animals at 8 h after exposure to the dust. We also measured bronchial reactivity in sensitized exposed guinea pigs at 2 h (n = 5) and at 24 h (n = 5). Pulmonary inflation pressure (PIP) was used to assess bronchopulmonary response. Blood samples were taken for assessment of IgG-1 antibodies to TMA conjugated to guinea-pig albumin. The concentration of acetylcholine required to induce a 100% increase in PIP was used to assess bronchial reactivity. The lungs were eviscerated for histologic examination. All guinea pigs injected intradermally with TMA had high titers of specific IgG-1 antibodies to TMA conjugated to guinea-pig albumin. There was a significant increase in bronchial reactivity in sensitized guinea pigs 8 h after exposure to the TMA dust compared with that in the control animals. There was also a significant eosinophilic inflammatory influx in the subepithelium of the sensitized groups.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Thoracic Medicine, Royal Brompton National Heart & Lung Hospital, National Heart & Lung Institute, London, United Kingdom.
authors.forename: J P
authors.initials: JP
authors.lastname: Hayes
authors.forename: R
authors.initials: R
authors.lastname: Daniel
authors.forename: R D
authors.initials: RD
authors.lastname: Tee
authors.forename: P J
authors.initials: PJ
authors.lastname: Barnes
authors.forename: A J
authors.initials: AJ
authors.lastname: Taylor
authors.forename: K F
authors.initials: KF
authors.lastname: Chung
mesh_terms: D000393:Air Pollutants / Q000009:adverse effects*; D000403:Airway Resistance; D000818:Animals; D000906:Antibodies / Q000097:blood; D001706:Biopsy; D016535:Bronchial Hyperreactivity / Q000097:blood / Q000139:chemically induced* / Q000473:pathology; D004195:Disease Models, Animal; D004391:Dust; D004797:Enzyme-Linked Immunosorbent Assay; D004804:Eosinophils; D005069:Evaluation Studies as Topic; D006168:Guinea Pigs; D007114:Immunization; D007074:Immunoglobulin G / Q000276:immunology; D008297:Male; D010784:Photomicrography; D010796:Phthalic Anhydrides / Q000009:adverse effects*
chemical_list: D000393:Air Pollutants; D000906:Antibodies; D004391:Dust; D007074:Immunoglobulin G; D010796:Phthalic Anhydrides; C015559:trimellitic anhydride