Flow cytometric analysis of peripheral blood erythrocyte chimerism in alpha-thalassemic mice.
A rapid and reliable method for longitudinal studies on the degree of red cell chimerism following bone marrow transplantation of alpha-thalassemic recipient mice is presented. Blood obtained by tail clipping from transplanted mice was analyzed by measuring forward light scatter (FLS) distribution of red cells using a flow cytometer. Amplification and threshold of FLS were specifically adjusted. For flow cytometric analysis, the red cells needed to be suspended in hypotonic saline (103 mmol/l NaCl). Osmotic fragility testing showed that lysis of erythrocytes did not significantly influence the measurements. Flow cytometric measurement allowed for a rapid determination of the degree of red cell chimerism.
van den Bos
C
C
Department of Radiobiology, Erasmus University, Rotterdam, The Netherlands.
van Gils
F C
FC
Bartstra
R W
RW
Wagemaker
G
G
D000818:Animals; D016026:Bone Marrow Transplantation; D048429:Cell Size; D004912:Erythrocytes / Q000648:ultrastructure*; D004913:Erythrocytes, Abnormal / Q000648:ultrastructure; D004920:Erythropoiesis; D005434:Flow Cytometry; D006085:Graft Survival; D008137:Longitudinal Studies; D051379:Mice; D008807:Mice, Inbred BALB C; D009391:Nephelometry and Turbidimetry; D009996:Osmotic Fragility; D011184:Postoperative Period; D011828:Radiation Chimera; D014184:Transplantation, Homologous; D014916:Whole-Body Irradiation; D017085:alpha-Thalassemia / Q000097:blood* / Q000601:surgery