Inhibition of binding of bacteria to amniochorionic membranes by amniotic fluid.
The immunological composition of amniotic fluids is shown to be of such a lower order of activity that its role in fetal protection may be limited. Also, amniotic fluids were found not to have classical antibiotic activity. Amniotic fluids (25/31), however, were found to inhibit, by 27.5% to 88.2%, three target bacteria from binding to discs of amniochorionic membranes. This inhibition is also demonstrable with the monosaccharides alpha-D(+)-fucose, D(+)-galactose, alpha-D-glucose, alpha-D-lactose and bovine serum albumin-lactose conjugate, whereas other glycoconjugates enhanced bacterial binding. This demonstrates that the test bacteria bind to the amniochorionic membranes using bacterial lectins. In intraamniotic infection bacterial lectins may be complexed by amniotic fluid glycoconjugates which prevent the bacteria from binding to the amniochorionic membranes. This would explain asymptomatic infection and in the absence or reduced levels of the glycoconjugates the bacteria would bind to the amniochorionic membranes giving rise to symptomatic infection.
Daunter
B
B
University of Queensland, Department of Obstetrics and Gynaecology, Royal Brisbane Hospital, Australia.
Forbes
K L
KL
Sanderson
B M
BM
Morrison
J
J
Wright
G
G
D000650:Amnion / Q000382:microbiology*; D000653:Amniotic Fluid / Q000502:physiology*; D001422:Bacterial Adhesion / Q000187:drug effects; D018407:Bacterial Physiological Phenomena; D002823:Chorion / Q000382:microbiology*; D005260:Female; D005643:Fucose / Q000494:pharmacology; D005690:Galactose / Q000494:pharmacology; D005947:Glucose / Q000494:pharmacology; D006001:Glycoconjugates / Q000378:metabolism; D006801:Humans; D007785:Lactose / Q000494:pharmacology; D011247:Pregnancy
D006001:Glycoconjugates; D005643:Fucose; D005947:Glucose; D007785:Lactose; D005690:Galactose