Partial reactions of bacterial D-amino acid transaminase with asparagine substituted for the lysine that binds coenzyme pyridoxal 5'-phosphate.
In bacterial D-amino acid transaminase (EC 2.6.1.21) replacement of Lys-145, which is covalently linked to the coenzyme pyridoxal 5'-phosphate in the wild-type enzyme, by an Asn residue gave a mutant enzyme (K145N) that slowly performed each half-reaction, as determined by spectral measurements. With the wild-type enzyme, the kinetics of these events were so rapid that pre-steady-state conditions were needed for their determination. The internal aldimine between coenzyme and Lys-145 was rapidly reduced with NaCNBH3 in the wild-type enzyme, whereas in the mutant enzyme the coenzyme, which is not covalently linked to the protein, was more resistant to reduction; the reduced forms of both wild-type and mutant enzymes were inactive. With large amounts of the K145N mutant enzyme and either amino acid or keto acid substrate alone, the formation of some reaction intermediates, i.e., the external aldimine with D-alanine and the ketimine with alpha-ketoglutarate, can be measured by conventional spectroscopy. Suicide substrates also induced slow spectral shifts of the E-PLP form of the enzyme. For the K145N enzyme, exogenous amines affected only the rate of the transaldimination but not the removal of the alpha-proton of the substrate. These results suggest that in the mutant enzyme some amino acid side chain other than Lys-145 performs this function. In order to identify this site, the K145N mutant enzyme was completely inactivated by the radiolabeled suicide substrate D-serine. Peptide mapping of tryptic digests showed that Lys-267 was the modified site.(ABSTRACT TRUNCATED AT 250 WORDS)
Yoshimura
T
T
Rockefeller University, New York, New York.
Bhatia
M B
MB
Manning
J M
JM
Ringe
D
D
Soda
K
K
D000409:Alanine / Q000494:pharmacology; D001216:Asparagine / Q000737:chemistry* / Q000378:metabolism; D001483:Base Sequence; D001665:Binding Sites; D001894:Borohydrides / Q000494:pharmacology; D002384:Catalysis; D051263:D-Alanine Transaminase; D019856:Ethanolamine; D004983:Ethanolamines / Q000494:pharmacology; D001411:Geobacillus stearothermophilus / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D010449:Peptide Mapping; D011732:Pyridoxal Phosphate / Q000378:metabolism*; D013053:Spectrophotometry; D013329:Structure-Activity Relationship; D000637:Transaminases / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000378:metabolism*; D015091:beta-Alanine / Q000031:analogs & derivatives / Q000494:pharmacology
D001894:Borohydrides; D004983:Ethanolamines; D015091:beta-Alanine; C006977:3-chloroalanine; D019856:Ethanolamine; D011732:Pyridoxal Phosphate; D001216:Asparagine; C009282:sodium cyanoborohydride; D000637:Transaminases; D051263:D-Alanine Transaminase; D000409:Alanine