[Immunohistochemical detection of insulin and insulin-like growth factors using antibodies to various parts of the insulin receptor molecule].
The indirect peroxidase-labeled antibody method was used to localize insulin receptors (IR) in the liver and brain of rats under various physiological conditions. Light- and electron-microscopically, specific immunohistochemical reaction was revealed in hepatocytes and endothelial cells of brain vessels with antisera against alpha- and beta-subunits of IR. Immunohistochemical reaction in the hypothalamic median eminence was observed only with antiserum to peptides with amino acid sequence corresponding to sequence 48-77 of human placenta IR. Electron-microscopically, immunohistochemical reaction was revealed on the cytoplasmic membranes and granular endoplasmic reticulum of the fibroblasts in the pericapillary space. The receptors observed correspond to receptors to insulin-like growth factor-1.
Babichenko
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D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D001921:Brain / Q000098:blood supply; D001923:Brain Chemistry / Q000502:physiology*; D004730:Endothelium, Vascular / Q000737:chemistry / Q000166:cytology; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D008099:Liver / Q000737:chemistry* / Q000166:cytology; D010920:Placenta / Q000737:chemistry; D011247:Pregnancy; D051381:Rats; D017208:Rats, Wistar; D017526:Receptor, IGF Type 1 / Q000032:analysis*; D011972:Receptor, Insulin / Q000032:analysis*; D017386:Sequence Homology, Amino Acid
D000911:Antibodies, Monoclonal; D017526:Receptor, IGF Type 1; D011972:Receptor, Insulin
Immunogistokhimicheskoe vyiavlenie retseptorov k insulinu i insulinopodobnomu rostovomu faktoru I s pomoshch'iu antitel k razlichnym uchastkam molekuly insulinovogo retseptora.