Quantitative determination of glucose in blood plasma and in fruit juices by combined WATR-CPMG 1H NMR spectroscopy.
The quantitative analysis of pure glucose solution < or = 225 mM (< or = 40.8 mg/mL) in 90/10 H2O/D2O was successfully completed in dilute aqueous solution by the WATR-CPMG method whereby the T2 of the water resonance is manipulated by the WATR method followed by elimination of the water peak by the CPMG pulse sequence. The method was applied to the quantitative analysis of total glucose in blood plasma from human subjects undergoing the oral glucose tolerance test in the teaching hospital, and the results were compared to those obtained using a standard glucose oxidase method in a hospital chemical pathology laboratory. The accuracy of the results obtained using the WATR-CPMG method were generally within 5% of the glucose oxidase method. The coefficient of variation was determined to be better than 4% using plasma samples of diabetic subjects. Application to the quantitative analysis of orange and guava juice was also successfully demonstrated.
Fan
S
S
Department of Chemistry, Chinese University of Hong Kong, Shatin, New Territories.
Choy
W Y
WY
Lam
S L
SL
Au-Yeung
S C
SC
Tsang
L
L
Cockram
C S
CS
D001628:Beverages; D001786:Blood Glucose / Q000032:analysis*; D002957:Citrus / Q000737:chemistry; D005583:Fourier Analysis; D005951:Glucose Tolerance Test; D006801:Humans; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*
D001786:Blood Glucose