FTIR evidence for alcohol binding and dehydration in phospholipid and ganglioside micelles.
We theorize that intoxicants and modern anesthetics bind at the membrane-water interface and displace (dehydrate) bound water molecules by breaking the hydrogen bonds. We tested this hypothesis by examining the effect of butanol on the binding of water to the polar regions of lipids in reversed micelles. Understanding the mechanisms of intoxication requires studies in physiologically relevant systems such as systems containing sialoglycoconjugates, especially gangliosides, which concentrate in the synapses of neural tissue. Therefore, we compared butanol effects on phospholipid with effects on ganglioside. Hydrogen-bond breaking activity of 1-butanol was studied in reversed micelles made of dipalmitoylphosphotidylcholine (DPPC), ganglioside (GM1 and GT1b) or the lipid mixture in a D2O-CCl4 medium. Fourier transform infrared spectroscopy (FTIR) data indicated that 1-butanol binds to DPPC and to gangliosides. Adding GM1 to the DPPC micelles introduces a new binding site for the alcohol. GT1b binds more butanol than GM1, because of more binding sites provided by extra sialic acid moieties. Spectral red shifts indicate that both water and butanol bind to the C = O group of sialic acid. Butanol partially releases the surface-bound water by disrupting hydrogen bonds, as indicated by an appearance of a sharp new free OD stretching band of the released D2O molecules. However, control studies with lipid-free systems in CCl4 revealed that a free OD peak could occur from a deuterium exchange reaction between D2O and 1-butanol(ol-h).(ABSTRACT TRUNCATED AT 250 WORDS)
Yurttaş
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Department of Chemical Engineering, Texas A&M University, College Station 77843-4458.
Dale
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BE
Klemm
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WR
D015060:1,2-Dipalmitoylphosphatidylcholine / Q000502:physiology; D020001:1-Butanol; D000435:Alcoholic Intoxication / Q000503:physiopathology*; D000440:Butanols / Q000493:pharmacokinetics; D003681:Dehydration / Q000503:physiopathology*; D000431:Ethanol / Q000493:pharmacokinetics*; D005583:Fourier Analysis; D005732:Gangliosides / Q000502:physiology*; D006801:Humans; D006860:Hydrogen Bonding; D008051:Lipid Bilayers; D008560:Membrane Fluidity / Q000187:drug effects* / Q000502:physiology; D008563:Membrane Lipids / Q000502:physiology*; D008567:Membranes, Artificial; D010743:Phospholipids / Q000502:physiology*; D012815:Signal Processing, Computer-Assisted / Q000295:instrumentation; D013055:Spectrophotometry, Infrared / Q000295:instrumentation
D000440:Butanols; D005732:Gangliosides; D008051:Lipid Bilayers; D008563:Membrane Lipids; D008567:Membranes, Artificial; D010743:Phospholipids; D015060:1,2-Dipalmitoylphosphatidylcholine; D000431:Ethanol; D020001:1-Butanol