[Synthesis and expression in Escherichia coli of the gene for the albumin-binding domain of Streptococcus protein G].
The chemical-enzymatic synthesis of a gene coding for A2B2 repeats of the albumin-binding domain of streptococcal protein G has been accomplished. The codon usage of the natural gene has been modified to adapt an artificial sequence for the efficient translation in E. coli. The gene (238 b.p.) was cloned in the polylinker plasmid pUCL1 and then fused in frame to the 3'-terminus of the gene for the IgG-binding domain of staphylococcal protein A, which was earlier cloned in the expression plasmid pUCL2. A fused polypeptide composed of the E and B domains of protein A and A2B2 repeats of protein G was produced in E. coli cells under the lac promoter control. The resulted product was isolated by affinity chromatography on IgG-sepharose and (or) albumin-sepharose.
Chakhmakhcheva
O G
OG
Foti
D
D
Pashkova
I N
IN
Polushin
N N
NN
Efimov
V A
VA
D000418:Albumins / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D002846:Chromatography, Affinity; D004247:DNA; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics*; D008969:Molecular Sequence Data; D010957:Plasmids; D012091:Repetitive Sequences, Nucleic Acid; D013291:Streptococcus / Q000378:metabolism*
D000418:Albumins; D001426:Bacterial Proteins; C034284:IgG Fc-binding protein, Streptococcus; D004247:DNA
Sintez i ékspressiia v Escherichia coli gena al'buminsviazyvaiushchegodomena belka G Streptococcus.