title: Frameshifting in the expression of the Escherichia coli trpR gene.
abstract: The trpR gene of Escherichia coli carries an open reading frame that encodes the trp repressor, 108 amino acids long. Here we show that translation of an additional (+1) reading frame of trpR occurs both in vivo and in vitro. This results in the synthesis of a stable +1 frame polypeptide. Using site-specific mutagenesis, immunological techniques and amino acid sequencing we have found that the N-terminus of the +1 frame product and that of the known 0 frame product are identical but that their C-termini differ. Our results are discussed in relation to the role of natural frameshifting as a regulatory mechanism of gene expression in general, and with respect to tryptophan biosynthesis in particular.
authors.affiliation: Department of Molecular Biology, Hebrew University, Hadassah Medical School, Jerusalem, Israel.
authors.forename: I
authors.initials: I
authors.lastname: Benhar
authors.forename: C
authors.initials: C
authors.lastname: Miller
authors.forename: H
authors.initials: H
authors.lastname: Engelberg-Kulka
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics*; D001483:Base Sequence; D003062:Codon; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D016366:Open Reading Frames; D014176:Protein Biosynthesis; D012097:Repressor Proteins / Q000096:biosynthesis / Q000235:genetics*; D014364:Tryptophan / Q000235:genetics*; D029968:Escherichia coli Proteins
chemical_list: D001426:Bacterial Proteins; D003062:Codon; D012097:Repressor Proteins; D014364:Tryptophan; C027190:TRPR protein, E coli; D029968:Escherichia coli Proteins