Hepatic metastasis alters the immune function of murine liver nonparenchymal cells.
To examine the effect of a single hepatic focus of metastatic colon tumor on the immune function of liver non-parenchymal cells (NPCs) from C57Bl/6 mice, we injected 2.5 x 10(5) liver-derived murine colon adenocarcinoma (LD-MCA-38) cells beneath the liver capsule. Three weeks following injection of the tumor cells, the immune function of the NPCs was studied. The NPCs from tumor-bearing mice exhibited increased cytotoxic and proliferative activity. The NPCs from tumor-bearing mice also contained a greater percentage of CD8+ and T-cell receptor gamma/delta+ liver-associated T lymphocytes. Levels of interleukin 6 and tumor necrosis factor were increased in the NPC supernatant, and interleukin 6 levels were increased in serum from tumor-bearing mice. We conclude that the presence of a single hepatic focus of metastatic tumor results in augmented immune function of murine liver NPCs.
Johnkoski
J A
JA
Department of Surgery, State University of New York, Buffalo.
Tzung
S P
SP
Doerr
R J
RJ
Cohen
S A
SA
D000230:Adenocarcinoma / Q000473:pathology; D000818:Animals; D001681:Biological Assay; D001853:Bone Marrow / Q000276:immunology; D001854:Bone Marrow Cells; D016827:CD8 Antigens / Q000737:chemistry; D002455:Cell Division / Q000276:immunology; D003110:Colonic Neoplasms / Q000473:pathology; D004195:Disease Models, Animal; D005069:Evaluation Studies as Topic; D005434:Flow Cytometry; D015850:Interleukin-6 / Q000097:blood / Q000737:chemistry / Q000502:physiology; D008099:Liver / Q000737:chemistry / Q000166:cytology / Q000276:immunology*; D008114:Liver Neoplasms, Experimental / Q000097:blood / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D013154:Spleen / Q000166:cytology / Q000276:immunology; D016176:T-Lymphocyte Subsets / Q000737:chemistry; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000737:chemistry / Q000502:physiology
D016827:CD8 Antigens; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha