title: Transcription map of the early region of the Streptomyces bacteriophage phi C31.
abstract: Streptomyces coelicolor A3(2), lysogenised by the temperature-sensitive cts1 mutant of phi C31, can be synchronously induced into the lytic cycle by heat treatment. A transcription map of 10 kb of the phi C31 early gene cluster was deduced using low-resolution S1 nuclease mapping of RNA prepared 10 min after induction. At least nine early transcripts, early (e)RNAs 1-9, were localised reading exclusively rightwards with respect to the standard physical map of phi C31. The mRNAs were extensively overlapping, frequently initiating at the same place but terminating at different sites, and vice versa. Gene expression during the lytic cycle was tightly regulated; no transcription was observed before induction. Transcription was maximal at 10 min post-induction, and at 20 min, eRNAs 5 and 6 persisted whilst eRNAs 7-9 were severely reduced or absent. The pattern of transcription of the early region is consistent with the simultaneous activation of a large number of promoters and differential termination efficiency.
authors.affiliation: Department of Biological and Molecular Science, University of Stirling, Scotland, UK.
authors.forename: C J
authors.initials: CJ
authors.lastname: Ingham
authors.forename: M C
authors.initials: MC
authors.lastname: Smith
mesh_terms: D001435:Bacteriophages / Q000235:genetics*; D002874:Chromosome Mapping; D004587:Electrophoresis, Agar Gel; D015967:Gene Expression Regulation, Viral; D005814:Genes, Viral; D005810:Multigene Family; D010957:Plasmids; D012367:RNA, Viral / Q000235:genetics; D013302:Streptomyces; D013728:Terminator Regions, Genetic; D014158:Transcription, Genetic
chemical_list: D012367:RNA, Viral