Three clustered origins of replication in a promiscuous-plasmid replicon and their differential use in a PolA+ strain and a delta PolA strain of Escherichia coli K-12.
A 1,197-bp region of the broad-host-range plasmid pCU1 is adequate for its replication. Analysis of replicating molecules containing this region reveals three clustered origins of vegetative replication and replication proceeds bidirectionally from each in a theta mode. In an Escherichia coli polymerase I deletion mutant, utilization of one of these three origins was not detected. The potentiality for origin utilization may therefore be a determinant of replicon host range.
Banerjee
S K
SK
Department of Biology, Carleton University, Ottawa, Ontario, Canada.
Luck
B T
BT
Kim
H Y
HY
Iyer
V N
VN
D003001:Cloning, Molecular; D004256:DNA Polymerase I / Q000235:genetics* / Q000378:metabolism; D004261:DNA Replication; D004269:DNA, Bacterial / Q000096:biosynthesis / Q000648:ultrastructure; D004926:Escherichia coli / Q000235:genetics* / Q000648:ultrastructure; D008854:Microscopy, Electron; D009154:Mutation; D010957:Plasmids; D012093:Replicon
D004269:DNA, Bacterial; D004256:DNA Polymerase I