Ozone-induced changes of mRNA levels of beta-1,3-glucanase, chitinase and 'pathogenesis-related' protein 1b in tobacco plants.
Treatment of the ozone-sensitive tobacco cultivar Bel W3 with an ozone pulse (0.15 microliter/l, 5 h) markedly increased the mRNA level of basic beta-1,3-glucanase and to a lower degree that of basic chitinase. The increase of beta-1,3-glucanase mRNA level occurred within 1 h and showed a transient maximum. Seventeen hours after ozone treatment, the beta-1,3-glucanase mRNA level decreased to lower values. The increase of basic chitinase mRNA level was delayed and was less pronounced than that of beta-1,3-glucanase mRNA. Cultivar Bel B showed only a small increase of beta-1,3-glucanase mRNA level after the same ozone treatment, whereas its basic chitinase mRNA was more strongly induced. Prolonged ozone treatment for 2 days of tobacco Bel W3 led to a persistent level of beta-1,3-glucanase and basic chitinase mRNAs, as well as to an increase of acidic chitinase and 'pathogenesis-related' (PR) 1b mRNA levels. The results indicate that genes so far considered to code for PR proteins may also be involved in the plant response to oxidative stress.
Ernst
D
D
GSF-Forschungszentrum für Umwelt und Gesundheit GmbH, Institut für Biochemische Pflanzenpathologie, Neuherberg, FRG.
Schraudner
M
M
Langebartels
C
C
Sandermann
H
H
D015152:Blotting, Northern; D002688:Chitinases / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation; D043326:Glucan 1,3-beta-Glucosidase; D007700:Kinetics; D010126:Ozone / Q000494:pharmacology*; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D010947:Plants, Toxic; D012333:RNA, Messenger / Q000235:genetics; D014026:Nicotiana / Q000201:enzymology / Q000235:genetics*; D001617:beta-Glucosidase / Q000235:genetics* / Q000378:metabolism
D010940:Plant Proteins; D012333:RNA, Messenger; C053376:pathogenesis-related proteins, plant; D010126:Ozone; D002688:Chitinases; D001617:beta-Glucosidase; D043326:Glucan 1,3-beta-Glucosidase