The isolation and sequence of sheep interleukin 4.
A cDNA corresponding to sheep IL-4 has been isolated and sequenced. The cDNA was synthesized using PCR and oligonucleotides designed from conserved regions of IL-4 cDNA published sequences. The nucleotide and amino acid sequences of a sheep IL-4 cDNA are presented and compared with sequences from other species. At the nucleic acid level, the sheep cDNA shared 73 and 62% homology with human and mouse cDNA homologues, respectively, while comparisons at the amino acid level revealed 50% homology with human IL-4 and 33% with the mouse homologue. Total amino acid homology between these species was 27%.
Engwerda
C R
CR
School of Agriculture, LaTrobe University, Bundoora, Victoria, Australia.
Sandeman
R M
RM
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA; D006801:Humans; D015847:Interleukin-4 / Q000235:genetics* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D012756:Sheep
D015847:Interleukin-4; D004247:DNA