[The use of DNA polymerase from Thermus thermophilus].
National DNA polymerase from Thermus thermophilus was used in polymerase chain reaction (PCR). The synthetic oligonucleotides (primers) to the basic structural HIV genes GAG, ENV, the genome DNA of donor peripheral blood lymphocytes were used, and the controls included the plasmid DNA with cloned HIV genome and the genome DNA of peripheral blood lymphocytes from HIV-infected persons confirmed by ELISA and Western blot analysis. The PCR technique and evaluation of the obtained results are described. The expediency of using PCR for different contingents is discussed.
Kuz'min
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Kuz'min
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Morozov
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D000328:Adult; D004247:DNA / Q000097:blood / Q000235:genetics; D004259:DNA-Directed DNA Polymerase / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005814:Genes, Viral; D015483:HIV Antibodies / Q000097:blood; D015497:HIV-1 / Q000235:genetics; D006801:Humans; D008214:Lymphocytes / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods; D016963:Thermus thermophilus / Q000201:enzymology*; D015678:Viral Structural Proteins / Q000235:genetics
D015483:HIV Antibodies; D015678:Viral Structural Proteins; D004247:DNA; D004259:DNA-Directed DNA Polymerase
Primenenie DNK-polimerazy iz Thermus termophilus.