Increased expression of the negative growth factor, galactoside-binding protein, gene in transformed thyroid cells and in human thyroid carcinomas.
Murine beta-galactoside-binding protein has been shown to be a cell growth regulatory molecule and a cytostatic factor. We analysed the beta-galactoside-binding protein gene expression in a thyroid cell system including two normal cell lines (FRTL-5 and PC Cl 3) and the same cells transfected by several oncogenes that induce different degrees of malignancy and differentiation. We show that beta-galactoside-binding protein mRNA levels correlate with the expression of the malignant phenotype. Run-on experiments suggest that a transcriptional effect accounts at least in part for such a difference. We also show that the beta-galactoside-binding protein gene expression is increased in most human papillary thyroid carcinomas compared with normal thyroid.
Chiariotti
L
L
Dipartimento di Biologia e Patologia Cellulare e Molecolare L. Califano, Università degli Studi di Napoli, Italy.
Berlingieri
M T
MT
De Rosa
P
P
Battaglia
C
C
Berger
N
N
Bruni
C B
CB
Fusco
A
A
D017500:Adenovirus E1A Proteins / Q000235:genetics; D002454:Cell Differentiation; D002460:Cell Line; D002461:Cell Line, Transformed; D002471:Cell Transformation, Neoplastic; D005697:Galactosides / Q000378:metabolism; D037161:Galectins; D005814:Genes, Viral; D016259:Genes, myc; D011905:Genes, ras; D016391:Genes, src; D006131:Growth Inhibitors / Q000235:genetics*; D006388:Hemagglutinins / Q000235:genetics*; D006801:Humans; D009857:Oncogenes; D011519:Proto-Oncogenes; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D013961:Thyroid Gland / Q000502:physiology*; D013964:Thyroid Neoplasms / Q000235:genetics*; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured
D017500:Adenovirus E1A Proteins; D005697:Galactosides; D037161:Galectins; D006131:Growth Inhibitors; D006388:Hemagglutinins; D012333:RNA, Messenger