title: Response of arterial smooth muscle cells to laminar flow.
abstract: This study was designed to determine whether laminar flow influences the proliferation rate and morphology of smooth muscle cells (SMC) in culture. Bovine aortic SMC were subjected to a shear stress of 6 dyne/cm2. The control group was subjected to similar incubation conditions without flow. Flow cytometry demonstrated decreased proliferation rate in SMC subjected to laminar flow. This phenomenon was still evident 24 hours after flow cessation. SMC were examined by light and electron microscopy. SMC subjected to laminar flow aligned along the direction of flow assuming a spheric morphology. These changes were reversible after a 48-hour resting period. The degree of organization of actin, tubulin and other microfilaments was evaluated by indirect immunofluorescence. SMC subjected to shear stress showed a clear reorganization of the cytoskeleton with expression of stress fibres. These changes were reversible after a 48-hour resting period. These findings may contribute to understanding the mechanisms by which SMC in vivo respond to forces generated by blood flowing under pressure.
authors.affiliation: I Istituto di Clinica Chirurgica, University La Sapienza Medical School, Rome, Italy.
authors.forename: A V
authors.initials: AV
authors.lastname: Sterpetti
authors.forename: A
authors.initials: A
authors.lastname: Cucina
authors.forename: L S
authors.initials: LS
authors.lastname: D'Angelo
authors.forename: B
authors.initials: B
authors.lastname: Cardillo
authors.forename: A
authors.initials: A
authors.lastname: Cavallaro
mesh_terms: D008841:Actin Cytoskeleton / Q000648:ultrastructure; D000199:Actins / Q000648:ultrastructure; D000818:Animals; D001013:Aorta, Thoracic / Q000166:cytology* / Q000502:physiology / Q000648:ultrastructure; D002417:Cattle; D002455:Cell Division; D003599:Cytoskeleton / Q000648:ultrastructure; D004730:Endothelium, Vascular / Q000166:cytology* / Q000502:physiology / Q000648:ultrastructure; D005069:Evaluation Studies as Topic; D005434:Flow Cytometry; D006439:Hemodynamics; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D009131:Muscle, Smooth, Vascular / Q000166:cytology* / Q000502:physiology / Q000648:ultrastructure; D012212:Rheology; D013314:Stress, Mechanical; D013997:Time Factors; D014404:Tubulin / Q000648:ultrastructure
chemical_list: D000199:Actins; D014404:Tubulin