Research note: the reactivity of anti-chondroitin sulfate antibody to comb and wattle galactosaminoglycans.
The current study was undertaken to examine the reactivity of anti-chondroitin sulfate monoclonal antibody, CS-56, to the comb and wattle galactosaminoglycans containing varying proportions of L-iduronic acid. Galactosaminoglycans were isolated from mature rooster comb and wattle tissues and were separated into four fractions (I, II, III, and IV), which were precipitable with 18, 25, 40, and 50% ethanol, respectively. Each fraction was examined using an ELISA technique. The results demonstrated presence of CS-56 epitope in galactosaminoglycans from both tissues. Most (> 99%) of the epitope were found in Fractions III and IV, which were galactosaminoglycans rich in D-glucuronic acid. Fractions I and II, which were dermatan sulfates rich in L-iduronic acid, showed little or very weak antigenicities.
Nakano
T
T
Department of Animal Science, University of Alberta, Edmonton, Canada.
Sim
J S
JS
D000818:Animals; D000911:Antibodies, Monoclonal; D002645:Chickens; D002809:Chondroitin Sulfates / Q000032:analysis; D003129:Comb and Wattles / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay; D007067:Iduronic Acid / Q000032:analysis; D008297:Male; D011134:Polysaccharides / Q000032:analysis*
D000911:Antibodies, Monoclonal; D011134:Polysaccharides; D007067:Iduronic Acid; D002809:Chondroitin Sulfates; C007815:galactosaminoglycan