[The use of an immunohistochemical method for detecting N7-methylguanine in human cells].
An immunohistochemical method, followed by silvering (Ag-treatment) of the final product of peroxidase reaction for N7-methylguanine detection, was first employed for human cells treated in vitro with N-methylnitrosourea. This method appeared to be highly specific and sensitive, compared to the routine immunohistochemical technique. Evidence has been provided on the application of Ag-treatment for detecting N7-methylguanine in the esophagus epithelium of patients with a high risk of cancer with this particular locality.
Mikheev
A M
AM
D002681:China; D004247:DNA / Q000187:drug effects / Q000378:metabolism; D004197:Disease Reservoirs; D004847:Epithelial Cells; D004848:Epithelium / Q000187:drug effects / Q000378:metabolism; D004938:Esophageal Neoplasms / Q000378:metabolism / Q000473:pathology; D004947:Esophagus / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D006147:Guanine / Q000031:analogs & derivatives* / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000166:cytology / Q000187:drug effects; D008770:Methylnitrosourea / Q000494:pharmacology; D012680:Sensitivity and Specificity; D016622:Silver Staining / Q000379:methods
D006147:Guanine; C008450:7-methylguanine; D008770:Methylnitrosourea; D004247:DNA
Ispol'zovanie immunogistokhimicheskogo metoda dlia vyiavleniia N7-metilguanina v kletkakh cheloveka.