title: Determination of ascorbic acid in pharmaceuticals and urine by reverse flow injection.
abstract: Two reverse flow injection (FI) methods, using spectrophotometric detection, are proposed for the determination of ascorbic acid. Both methods are based on its reaction with the ethylenediaminetetraacetic acid-CoIII complex in a medium of 5% diethylamine. In the first method, using the peak-height FI technique, ascorbic acid is determined over the range from 2 x 10(-4) to 5 x 10(-3) mol dm-3 and in the second, using the peak-width FI method, the working range is extended (2 x 10(-3)-5 x 10(-2) mol dm-3). Both FI methods were applied to the determination of ascorbic acid in pharmaceuticals while the peak-height FI technique was also used to determine ascorbic acid in urine.
authors.affiliation: Department of Analytical Chemistry, Faculty of Chemistry, University of Murcia, Spain.
authors.forename: M I
authors.initials: MI
authors.lastname: Albero
authors.forename: M
authors.initials: M
authors.lastname: Soledad García
authors.forename: C
authors.initials: C
authors.lastname: Sánchez-Pedreño
authors.forename: J
authors.initials: J
authors.lastname: Rodríguez
mesh_terms: D001205:Ascorbic Acid / Q000032:analysis* / Q000652:urine; D002614:Chelating Agents; D003035:Cobalt; D004492:Edetic Acid; D006801:Humans; D007202:Indicators and Reagents; D013053:Spectrophotometry / Q000295:instrumentation / Q000379:methods
chemical_list: D002614:Chelating Agents; D007202:Indicators and Reagents; C028222:cobalt-ethylenediamine tetraacetic acid chelate; D003035:Cobalt; D004492:Edetic Acid; D001205:Ascorbic Acid