title: A simple procedure for obtaining high-quality NMR spectra of semiquantitative value from small tissue specimens: cervical biopsies.
abstract: The handling of small tissue biopsy samples (< 100 microliters) for NMR investigations poses special problems. Optimal and stable positioning of the samples within the sensitive volume of the radiofrequency coil can be achieved by inserting the sample in a capillary. Methods for quantitation of the spectral information from samples requiring histological evaluation after the NMR experiment are discussed, in particular with respect to cervical biopsies.
authors.affiliation: Institute for Biodiagnostics, National Research Council of Canada, Winnipeg.
authors.forename: A C
authors.initials: AC
authors.lastname: Kuesel
authors.forename: T
authors.initials: T
authors.lastname: Kroft
authors.forename: J K
authors.initials: JK
authors.lastname: Saunders
authors.forename: M
authors.initials: M
authors.lastname: Préfontaine
authors.forename: N
authors.initials: N
authors.lastname: Mikhael
authors.forename: I C
authors.initials: IC
authors.lastname: Smith
mesh_terms: D001706:Biopsy; D002584:Cervix Uteri / Q000378:metabolism / Q000473:pathology*; D004867:Equipment Design; D005260:Female; D006801:Humans; D009682:Magnetic Resonance Spectroscopy / Q000295:instrumentation / Q000379:methods; D013048:Specimen Handling / Q000295:instrumentation / Q000379:methods; D002577:Uterine Cervical Diseases / Q000175:diagnosis / Q000473:pathology; D002583:Uterine Cervical Neoplasms / Q000175:diagnosis / Q000473:pathology