title: Particulate glycogen of mammalian liver: specificity in binding phosphorylase and glycogen synthase.
abstract: The glycogen particle - glycogen metabolizing enzyme complex was investigated to gain some understanding of its physiological significance. Fractionations of populations of particles from mouse liver were carried out utilising open column and high performance liquid chromatography, and based either on the molecular weight of the particles or the hydrophobic interactions of the glycogen-associated proteins. The activities of glycogen phosphorylase and glycogen synthase were measured in these fractions. Fractionations were of tissue in different stages of glycogen deposition or mobilization. In animals fed ad libitum, glycogen synthase was associated with the whole spectrum of molecular weights, while the glycogen phosphorylase distribution was skewed in favour of the lower molecular weight species. Under conditions of glycogen mobilization, the phosphorylase distribution changed to include all molecular weights. The hydrophobic interaction separations demonstrated that glycogen synthase binds to a specific subpopulation of particles that is a minor proportion of the total. In general, there was a direct relationship of the total amount of phosphorylase and synthase bound during periods of mobilization and deposition, respectively. Two notable exceptions were the large amounts of glucose-6-P dependent synthase present during the early period of glycogen mobilization and the high amounts of active phosphorylase appearing shortly after food withdrawal, in spite of interim glycogen deposition from presumably already ingested food.
authors.affiliation: Agriculture Canada Research Centre, London, Ont.
authors.forename: A
authors.initials: A
authors.lastname: Vardanis
mesh_terms: D000818:Animals; D002458:Cell Fractionation; D002850:Chromatography, Gel; D005260:Female; D005508:Food Deprivation; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D006006:Glycogen Synthase / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008112:Liver Glycogen / Q000378:metabolism*; D051379:Mice; D008970:Molecular Weight; D009097:Multienzyme Complexes / Q000378:metabolism*; D006005:Phosphorylases / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: D008112:Liver Glycogen; D009097:Multienzyme Complexes; D005954:Glucosephosphate Dehydrogenase; D006005:Phosphorylases; D006006:Glycogen Synthase