title: Metabolic relationships between proteins of myelin and paranodally shedded, partially degraded myelin fragments in the rabbit CNS.
abstract: The "close-to-node" regions of myelinated nerve fibres, i.e., the paranodal end segments, are generally thought to be sites of high metabolic activity and myelin sheath turnover. Data on turnover rates of individual myelin constituents are conflicting but there exists a common belief that myelin is metabolized as independent molecules rather than as a unit. The occurrence of paranodal Marchi-positive bodies, with morphological and biochemical properties consistent with partially degraded myelin, prompted us to examine the temporal dynamics of the incorporation of radioactive precursor label in the major proteins of myelin and the Marchi-positive bodies. 3H-leucine was administered intrathecally in adult rabbits. After various survival times, the spinal cord was subfractionated by ultracentrifugation in a discontinuous two-step 0.32 M/0.85 M sucrose gradient. Myelin was collected from the interface and a floating fraction, heavily enriched in Marchi-positive bodies, was recovered on top of the 0.32 M sucrose. By scintillation counting and by gel fluorography combined with immunoblotting, a gradual appearance with time of partially degraded peptides of myelin-associated protein and 2',3'-cyclic nucleotide 3'-phosphodiesterase was seen in the floating fraction but not in myelin. The temporal dynamics of the specific activities of these two proteins and myelin-basic protein and proteolipid protein were consistent with a typical source-product relationship between myelin and the material in the floating fraction. In conjunction with earlier morphological and biochemical findings, these data may suggest that Marchi-positive bodies appear as a consequence of myelin catabolism.
authors.affiliation: Department of Anatomy, University of Göteborg, Sweden.
authors.forename: H
authors.initials: H
authors.lastname: Persson
authors.forename: C H
authors.initials: CH
authors.lastname: Berthold
authors.forename: M
authors.initials: M
authors.lastname: Rydmark
authors.forename: C
authors.initials: C
authors.lastname: Fabricius
mesh_terms: D000818:Animals; D002458:Cell Fractionation; D002499:Centrifugation, Density Gradient; D015151:Immunoblotting; D007700:Kinetics; D007930:Leucine / Q000378:metabolism*; D009185:Myelin Proteins / Q000096:biosynthesis* / Q000302:isolation & purification; D009186:Myelin Sheath / Q000378:metabolism / Q000648:ultrastructure; D009413:Nerve Fibers, Myelinated / Q000378:metabolism* / Q000648:ultrastructure; D011817:Rabbits; D011865:Radioisotope Dilution Technique; D013116:Spinal Cord / Q000378:metabolism* / Q000648:ultrastructure; D014316:Tritium
chemical_list: D009185:Myelin Proteins; D014316:Tritium; D007930:Leucine