Smoking-related DNA adducts in human gastric cancers.
DNA was extracted from the tumour tissue of 26 patients (18 smokers, 8 non-smokers) undergoing surgery for gastric cancer, and analyzed for the presence of DNA adducts by the 32P-post-labelling method. Adducts were detected in all samples tested, and adduct levels ranged from 2 adducts/10(8) nucleotides to 60 adducts/10(8) nucleotides. In male subjects, adduct levels were significantly greater in the DNA of smokers than in that of non-smokers. These results support epidemiological data suggesting that smoking is a risk factor for gastric cancer.
Dyke
G W
GW
Dept. of Biology, University of York, UK.
Craven
J L
JL
Hall
R
R
Garner
R C
RC
D000368:Aged; D000369:Aged, 80 and over; D004273:DNA, Neoplasm / Q000378:metabolism*; D005260:Female; D005753:Gastric Mucosa / Q000378:metabolism; D006801:Humans; D008297:Male; D008875:Middle Aged; D010761:Phosphorus Radioisotopes; D012907:Smoking / Q000009:adverse effects / Q000378:metabolism*; D013274:Stomach Neoplasms / Q000209:etiology / Q000235:genetics* / Q000378:metabolism
D004273:DNA, Neoplasm; D010761:Phosphorus Radioisotopes