Spontaneous cluster formation of dendritic (veiled) cells and lymphocytes from skin lymph obtained from dogs with chronic lymphedema.
To examine the mechanism of spontaneous attachment of afferent lymph lymphocytes to dendritic cells (veiled), we sampled and tested cells from skin lymph in 3 dogs with chronic lymphedema. There were 3.3 +/- 2.8% veiled cells in clusters with lymphocytes in lymph obtained directly from dilated dermal lymphatics by percutaneous puncture. The number of ex vivo clusters forming in the collected lymph samples increased as a function of time and was temperature dependent. The ability of veiled cells to bind lymphocytes was independent of divalent cations but reduced by xylocaine and retinoic acid. Among various steroids tested only methylprednisolone showed an inhibitory effect on cluster formation. Indomethacin and acetylsalicylic acid had no blocking activity on cell binding. Moreover, no effect was seen by cyclosporin A and azathioprine. However, FK 506 had a potent inhibitory effect on spontaneous cluster formation. This study suggests that cluster formation by skin lymph veiled cells and lymphocytes is a spontaneous process which can be controlled in vitro.
Galkowska
H
H
Department of Surgical Research and Transplantation, Polish Academy of Sciences, Warsaw.
Olszewski
W L
WL
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D003713:Dendritic Cells / Q000187:drug effects / Q000502:physiology*; D004285:Dogs; D004492:Edetic Acid / Q000494:pharmacology; D007166:Immunosuppressive Agents / Q000494:pharmacology; D008012:Lidocaine / Q000494:pharmacology; D008196:Lymph / Q000166:cytology*; D008208:Lymphatic System / Q000473:pathology; D008209:Lymphedema / Q000473:pathology*; D008214:Lymphocytes / Q000187:drug effects / Q000502:physiology*; D012867:Skin / Q000473:pathology; D014212:Tretinoin / Q000494:pharmacology; D014700:Verapamil / Q000494:pharmacology
D000893:Anti-Inflammatory Agents; D007166:Immunosuppressive Agents; D014212:Tretinoin; D008012:Lidocaine; D004492:Edetic Acid; D014700:Verapamil