title: Development and persistence of Cowdria ruminantium specific antibodies following experimental infection of cattle, as detected by the indirect fluorescent antibody test.
abstract: Different breeds of cattle were experimentally infected with Palm River, a Zimbabwean isolate, or Ball-3, a South African isolate of Cowdria ruminantium, derived from tissue culture or tick or blood stabilates. C. ruminantium specific antibody responses were detected by an indirect fluorescent antibody test (IFAT) using C. ruminantium-infected bovine aortic endothelial (BAE) cell cultures as antigen. The first detection of antibodies to C. ruminantium generally coincided with the peak of the febrile reaction and the antibodies remained detectable for a period of 8-30 weeks in the Palm River infected group and 18-30 weeks in the Ball-3 infected group. Peak reciprocal antibody titres in both groups ranged from 64 to 2048 between 3 and 6 weeks post-infection. No apparent serological differences were observed among the various C. ruminantium isolates when tested in homologous and heterologous IFATs. Post-infection sera to Anaplasma marginale, Theileria parva parva, Babesia bigemina and Rickettsia conorii did not exhibit reactivity with the C. ruminantium antigen. These results indicate the possible use of C. ruminantium-infected cultures as antigen in IFATs to detect similar C. ruminantium-specific antibody responses in the field in clinically sick, recovered and carrier animals.
authors.affiliation: University of Florida/USAID/SADCC Heartwater Research Project, c/o Veterinary Research Laboratory, Causeway, Zimbabwe.
authors.forename: S M
authors.initials: SM
authors.lastname: Semu
authors.forename: S M
authors.initials: SM
authors.lastname: Mahan
authors.forename: C E
authors.initials: CE
authors.lastname: Yunker
authors.forename: M J
authors.initials: MJ
authors.lastname: Burridge
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis*; D000942:Antigens, Bacterial / Q000276:immunology; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology*; D002478:Cells, Cultured; D003429:Cross Reactions; D016995:Ehrlichia ruminantium / Q000276:immunology*; D004730:Endothelium, Vascular / Q000382:microbiology; D005455:Fluorescent Antibody Technique / Q000662:veterinary*; D006357:Heartwater Disease / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial