Effect of variations in temperature of injected saline on early development of chick embryo.
Development of chick embryo is sensitive to the incubation temperature and increase or decrease in incubation temperature produces defect in development but the effect of drug solution injected at variable temperatures on developing chick embryo has not been established so far. Fresh embryonated eggs were incubated and saline was administered at various temperatures after 17 hr of incubation. Embryos were harvested to see the effect of temperature variations of injected saline on neural tube formation. Normal neural tube development was seen only at 37 degrees C, hot or cold saline produced maldevelopment of neural tube. Thus it is concluded that any solution or drug which needs to be injected should ideally be used at 37 degrees C, as temperature lower or higher than that may vitiate the results.
Kotwani
A
A
Department of Pharmacology, Maulana Azad Medical College, New Delhi, India.
Mehta
V L
VL
Iyenger
B
B
D000818:Animals; D002490:Central Nervous System / Q000196:embryology*; D002642:Chick Embryo; D003080:Cold Temperature / Q000009:adverse effects*; D006358:Hot Temperature / Q000009:adverse effects*