Ergosterol depletion and 4-methyl sterols accumulation in the yeast Saccharomyces cerevisiae treated with an antifungal, 6-amino-2-n-pentylthiobenzothiazole.
In Saccharomyces cerevisiae treated with an antifungal agent, 6-amino-2-n-pentylthiobenzothiazole, levels of ergosterol and other 4-desmethylsterols were found to be significantly reduced. Major sterols in treated yeast were lanosterol, 4,4-dimethylzymosterol, 4-methylzymosterol and 4-methylfecosterol. A hypothesis is stated that the antifungal agent inhibits sterol demethylation at C-4 and forces the biosynthesis to a blind pathway ending by 4-methylfecosterol.
Kuchta
T
T
Food Research Institute, Modra, Czecho-Slovakia.
Bartková
K
K
Kubinec
R
R
D000935:Antifungal Agents / Q000494:pharmacology*; D004875:Ergosterol / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D007700:Kinetics; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D013261:Sterols / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism*; D013844:Thiazoles / Q000494:pharmacology*
D000935:Antifungal Agents; D013261:Sterols; D013844:Thiazoles; C078412:6-amino-2-n-pentylthiobenzothiazole; D004875:Ergosterol