Crystallization and preliminary crystallographic investigation of methanol dehydrogenase from Methylobacterium extorquens AM1.
Single crystals of methanol dehydrogenase (MDH) from Methylobacterium extorquens AM1 have been grown by the vapour diffusion method. These crystals diffract to beyond 2 A resolution and are suitable for X-ray crystallography. They belong to the orthorhombic space group P2(1)2(1)2(1) and have the following unit cell parameters: a = 66.79 A, b = 108.9 A, c = 188.9 A. One asymmetric unit contains an alpha 2 beta 2 tetramer of MDH and the location of the non-crystallographic 2-fold symmetry axis of this tetramer is defined by the paired positions of the binding sites of heavy atoms in four MDH-derivatives.
Ghosh
M
M
Laboratory of Molecular Biophysics, University of Oxford, U.K.
Harlos
K
K
Blake
C C
CC
Richardson
I
I
Anthony
C
C
D000429:Alcohol Oxidoreductases / Q000737:chemistry*; D003460:Crystallization; D006088:Gram-Negative Aerobic Bacteria / Q000201:enzymology*; D014961:X-Ray Diffraction
D000429:Alcohol Oxidoreductases; C019641:alcohol dehydrogenase (acceptor)