title: [Differences in anti-oncogene p53 expression in human monocytes and lymphocytes in vitro].
abstract: The p53 gene has been associated with malignant transformation as well as "anti-oncogene" activity. In the present report expression of p53 in resting and activated human blood monocytes and lymphocytes is analyzed. It is found that human monocytes freshly isolated by continuous percoll gradient centrifugation contained detectable level of p53 mRNA. Stimulation of monocytes by potent activation inducer Staphylococcus Aureus Cowan I for 3-5 hr caused disappearance of r53 mRNA. In contrast, induction of high level of TNF-alpha mRNA was detected. Addition of cycloheximide had no effect on p53 mRNA content in stimulated monocytes, and caused disappearance of mRNA in resting cells. In lymphocytes cultures p53 mRNA was absent in freshly isolated cells and in resting lymphocytes cultured for 20 hr. Activation of lymphocytes by lectin caused accumulation of p53 mRNA. We suggest that r53 gene regulation and functions might be different in human monocytes and lymphocytes.
authors.forename: O A
authors.initials: OA
authors.lastname: Osipovich
authors.forename: A B
authors.initials: AB
authors.lastname: Sudarikov
authors.forename: T S
authors.initials: TS
authors.lastname: Kolesnikova
authors.forename: N I
authors.initials: NI
authors.lastname: Misuno
authors.forename: N N
authors.initials: NN
authors.lastname: Voĭtenok
mesh_terms: D015152:Blotting, Northern; D002478:Cells, Cultured; D003470:Culture Media; D015870:Gene Expression; D016158:Genes, p53; D006801:Humans; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000648:ultrastructure*; D009000:Monocytes / Q000648:ultrastructure*; D012333:RNA, Messenger / Q000235:genetics; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
chemical_list: D003470:Culture Media; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha
vernacular_title: Razvitiia v ékspressii "antionkogena" p53 v monotsitakh i limfotsitakh cheloveka in vitro.