title: Effects of povidone-iodine chemical preparation and saline irrigation on the perilimbal flora.
abstract: OBJECTIVE
  To analyze the effects of 5% povidone-iodine preparation and saline irrigation on the species composition of perilimbal flora.
  
  
  METHODS
  Cultures were taken from the perilimbal conjunctiva in 100 eyes before preparation for ophthalmic surgery, after instillation of povidone-iodine solution, and after saline irrigation.
  
  
  RESULTS
  Bacteria were isolated in 75% of eyes before preparation, in 28% after povidone-iodine instillation, and in 24% after saline irrigation. Fifty-one culture-positive eyes became negative with povidone-iodine, while only four culture-negative eyes became culture-positive (P < 0.001). The number of eyes yielding coagulase-negative staphylococci, Staphylococcus aureus, and Propionibacterium were significantly decreased after povidone-iodine instillation. Twenty-three culture-positive eyes became negative after saline irrigation, while 19 culture-negative eyes became culture-positive (P > 0.25).
  
  
  CONCLUSIONS
  Povidone-iodine solution is effective in reducing bacterial recovery from the perilimbal conjunctiva, where most incisions for intraocular surgery occur. Saline irrigation after povidone-iodine preparation has no significant effect.
authors.affiliation: Department of Ophthalmology, University of Washington School of Medicine, Seattle.
authors.forename: D A
authors.initials: DA
authors.lastname: Boes
authors.forename: T D
authors.initials: TD
authors.lastname: Lindquist
authors.forename: T R
authors.initials: TR
authors.lastname: Fritsche
authors.forename: R E
authors.initials: RE
authors.lastname: Kalina
mesh_terms: D001419:Bacteria / Q000187:drug effects / Q000302:isolation & purification; D015169:Colony Count, Microbial; D003228:Conjunctiva / Q000382:microbiology*; D004203:Disinfection; D015818:Eye Infections, Bacterial / Q000188:drug therapy*; D006801:Humans; D016850:Limbus Corneae / Q000382:microbiology; D008828:Microbiological Techniques; D011206:Povidone-Iodine / Q000494:pharmacology*; D011300:Preoperative Care; D012965:Sodium Chloride; D007507:Therapeutic Irrigation
chemical_list: D012965:Sodium Chloride; D011206:Povidone-Iodine