Cu(2+)-induced lipid oxidation in plasma: questionable relation between cholesterol oxidation and LDL modification.
Oxidatively modified low-density lipoproteins (LDL) may be involved in the process of cholesterol deposition in arteries. Because of their cytotoxicity, oxysterols resulting from cholesterol oxidation could be a contributing factor in this process. Studies in this area have generally been performed on purified LDL, but in our research whole plasma was exposed to the oxidizing action of copper. Oxidation of the ring structure, which is at the origin of oxysterols, apparently occurs once most polyunsaturated fatty acids have disappeared. Hydrated LDL density reaches a value identical to that obtained during oxidation of LDL by endothelial cells, whereas the ring structure remains unmodified.
Tallineau
C
C
Laboratory of Biochemistry and Toxicology, Jean Bernard Hospital, Poitiers, France.
Pontcharraud
R
R
Guettier
A
A
Piriou
A
A
D000328:Adult; D002784:Cholesterol / Q000097:blood*; D002788:Cholesterol Esters / Q000097:blood; D002849:Chromatography, Gas; D003300:Copper / Q000494:pharmacology*; D004228:Dithionitrobenzoic Acid / Q000494:pharmacology; D006801:Humans; D015227:Lipid Peroxidation; D008055:Lipids / Q000097:blood*; D008077:Lipoproteins, LDL / Q000097:blood*
D002788:Cholesterol Esters; D008055:Lipids; D008077:Lipoproteins, LDL; D003300:Copper; D002784:Cholesterol; D004228:Dithionitrobenzoic Acid