title: [Isolation and identification of Gardnerella vaginalis in women with symptoms of bacterial vaginosis].
abstract: 70 samples of vaginal secretions were collected from women with bacterial vaginosis syndrome, that were attended in Nuevo Veranillo' Health Center. All samples were tested in order to determinate the presence of amine and to Gram'methods stain to observe the morphologic characteristics of the "Clue" cells (epithelial cells with adhered bacteria). Samples were cultured in a selective media of blood agar in Columbia base with colistine and nalidixic acid, and incubated in an environment of CO2 at 37 degrees C. Tests of oxidase, catalase, glucid fermentation, hippurate hydrolysis and starch hydrolysis were made on the grown colonies. 27 out of 70 samples resulted in positive cultures for Gardnerella vaginalis, 11 to pregnant women and 16 to nonpregnant women; among these, 14 women used some kind of contraceptive methods as DIU or pill.
authors.affiliation: Departamento de Microbiología, Facultad de Medicina, Universidad de Panamá.
authors.forename: X
authors.initials: X
authors.lastname: León
authors.forename: M
authors.initials: M
authors.lastname: Ruiz
authors.forename: N O
authors.initials: NO
authors.lastname: de Moreno
authors.forename: L
authors.initials: L
authors.lastname: de Richards
mesh_terms: D000293:Adolescent; D000328:Adult; D005260:Female; D006194:Gardnerella vaginalis / Q000302:isolation & purification*; D006801:Humans; D016585:Vaginosis, Bacterial / Q000382:microbiology*
vernacular_title: Aislamiento e identificación de Gardnerella vaginalis en mujeres con síntomas de vaginosis bacteriana.