title: Siderophores and related outer membrane proteins produced by pseudomonads isolated from eels and freshwater.
abstract: A total of 46 environmental pseudomonads, together with six type strains, were examined for their siderophore-producing activity. All strains were able to grow under iron-limiting conditions, gave orange halos in the CAS agar assay, and produced hydroxamates, and some of them also produced phenolate-type compounds. Bioassays showed that all strains, except Pseudomonas aeruginosa, promoted growth of mutant strain Arthrobacter flavescens JG-9, deficient in hydroxamate production, and some of them promoted growth of Salmonella typhimurium enb-1, which requires enterobactin for growth. The presence of iron-regulated outer membrane proteins was observed, the molecular size of the main induced proteins ranged between 76 and 93 kDa.
authors.affiliation: Departamento de Microbiología, Facultad de Ciencias Biológicas, Universitat de Valencia, Burjasot, Spain.
authors.forename: R
authors.initials: R
authors.lastname: Aznar
authors.forename: E
authors.initials: E
authors.lastname: Alcaide
mesh_terms: D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000096:biosynthesis* / Q000737:chemistry; D004524:Eels / Q000382:microbiology; D005618:Fresh Water; D007501:Iron / Q000378:metabolism; D008970:Molecular Weight; D011549:Pseudomonas / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*; D017262:Siderophores / Q000096:biosynthesis*; D013045:Species Specificity; D014871:Water Microbiology
chemical_list: D001425:Bacterial Outer Membrane Proteins; D017262:Siderophores; D007501:Iron