[Molecular cloning of the gene for secreted beta-galactosidase of the filamentous fungus Penicillium canescens].
A secreted beta-galactosidase gene from P. canescens was cloned using synthetic oligonucleotide probes and its nucleotide sequence was partially determined. The gene was shown to be present in the genome as a single copy. The deduced primary structure of the signal peptide and the first 25 amino acid residues of the mature protein was established. The structure analysis of the beta-galactosidase gene revealed at least one short intron 64 bp long. The transcription start point was found to be located 89 bp upstream the initiation codon. Some peculiarities of the signal peptide cleavage mechanism are discussed.
Nikolaev
I V
IV
Epishin
S M
SM
Zakharova
E S
ES
Kotenko
S V
SV
Vinetskiĭ
Iu P
IuP
D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004271:DNA, Fungal; D007438:Introns; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D010407:Penicillium / Q000201:enzymology*; D021382:Protein Sorting Signals / Q000235:genetics; D014158:Transcription, Genetic; D001616:beta-Galactosidase / Q000235:genetics*
D004271:DNA, Fungal; D015345:Oligonucleotide Probes; D021382:Protein Sorting Signals; D001616:beta-Galactosidase
Molekuliarnoe klonirovanie gena sekretiruemoĭ beta-galaktozidazy mitselial'nogo griba Penicillium canescens.