Yeast RNA polymerase II initiation factor e: isolation and identification as the functional counterpart of human transcription factor IIB.
Yeast RNA polymerase II initiation factor e was purified to homogeneity and identified by biochemical criteria as the counterpart of human transcription factor IIB. Factor e was essential for initiation of transcription from yeast and mammalian promoters in a reconstituted yeast transcription system. Activity resided in a single polypeptide of approximately 41 kDa, identified by peptide sequence analysis as the product of the SUA7 gene. Factor e interacted specifically with RNA polymerase II, consistent with a proposed role in determining the start site of transcription.
Tschochner
H
H
Department of Cell Biology, Fairchild Center, Stanford University School of Medicine, CA 94305.
Sayre
M H
MH
Flanagan
P M
PM
Feaver
W J
WJ
Kornberg
R D
RD
D000595:Amino Acid Sequence; D005656:Fungal Proteins / Q000302:isolation & purification* / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry; D011401:Promoter Regions, Genetic; D012319:RNA Polymerase II / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D012568:Schizosaccharomyces / Q000235:genetics; D035561:Transcription Factor TFIIA; D014157:Transcription Factors / Q000302:isolation & purification* / Q000378:metabolism; D014158:Transcription, Genetic
D005656:Fungal Proteins; D010446:Peptide Fragments; D012333:RNA, Messenger; D035561:Transcription Factor TFIIA; D014157:Transcription Factors; D012319:RNA Polymerase II