Trolox C, a lipid-soluble membrane protective agent, attenuates myocardial injury from ischemia and reperfusion.
The lipophilic antioxidant Trolox C, a vitamin E analog, was administered to isolated, buffer-perfused rabbit hearts subjected to 25 min of global stop-flow ischemia and 30 min of reperfusion. In six hearts, Trolox C (200 microM) was infused for 15 min immediately prior to ischemia and for the first 15 min of reperfusion. Six control hearts received only vehicle. Gas chromatography analysis confirmed that effective myocardial levels of Trolox were attained. At 30 min reperfusion, the recovery of left ventricular developed pressure was 56 +/- 3% of baseline in control hearts versus 70 +/- 4% in Trolox-treated hearts (p < .01). There was also significant improvement in recovery of Trolox-treated hearts in diastolic pressure and both maximum and minimum values of the first derivative of left ventricular pressure (dP/dt). Creatine phosphokinase release into the coronary effluent at 30 min of reperfusion was 16.5 +/- 8.4 IU/min in untreated and 6.3 +/- 1.0 IU/min (p < .05) in Trolox-treated hearts. Thus Trolox C, a lipophilic antioxidant, attenuated myocardial injury during stop-flow ischemia and reperfusion.
Rubinstein
J D
JD
Department of Medicine, University of Colorado, Health Sciences Center, Denver 80262.
Lesnefsky
E J
EJ
Byler
R M
RM
Fennessey
P V
PV
Horwitz
L D
LD
D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D002839:Chromans / Q000493:pharmacokinetics / Q000627:therapeutic use*; D003326:Coronary Circulation; D003402:Creatine Kinase / Q000378:metabolism; D003971:Diastole; D005260:Female; D016166:Free Radical Scavengers; D006861:Hydrogen Peroxide / Q000378:metabolism; D008055:Lipids; D008297:Male; D015428:Myocardial Reperfusion Injury / Q000503:physiopathology / Q000517:prevention & control*; D009206:Myocardium / Q000378:metabolism; D011817:Rabbits; D012995:Solubility; D013599:Systole; D016277:Ventricular Function, Left
D002839:Chromans; D016166:Free Radical Scavengers; D008055:Lipids; D006861:Hydrogen Peroxide; D003402:Creatine Kinase; C010643:6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid