Narine occlusion decreases basal levels of Fos protein in the cerebral cortex of the lizard Podarcis hispanica.
Immunocytochemical study of cerebral cortex of the lizard Podarcis hispanica using an antibody directed to the M peptide of the rat c-Fos protein showed a distinct pattern of Fos distribution. Abundant Fos-immunoreactive neuronal nuclei were detected in the cell layers of the medial, the dorsal and the lateral cortices, whereas only a few nuclei were found in the cell layer of the dorsomedial cortex. The Fos immunoreactivity was characterized by Western blot analysis of nuclear extracts from lizard brain and showed a distinct band with an apparent molecular weight of 30,000. In band-shift assays, nuclear extracts from lizard brain were shown to contain AP-1 complexes. The basal expression of Fos immunoreactivity is related to sensory olfactory input in the cerebral cortex of the lizard since experiments with olfactory-deprived animals resulted in a complete absence of Fos immunoreactivity in the cortical areas.
Blasco-Ibañez
J M
JM
Catedra de Citologia e Histologia, Facultad de Ciencias Biologicas, Universidad de Valencia, Spain.
Martinez-Guijarro
F J
FJ
Lopez-Garcia
C
C
Mellström
B
B
Naranjo
J R
JR
D000818:Animals; D015153:Blotting, Western; D002540:Cerebral Cortex / Q000033:anatomy & histology / Q000378:metabolism*; D007150:Immunohistochemistry; D008116:Lizards / Q000378:metabolism*; D009296:Nasal Cavity / Q000502:physiology*; D009830:Olfactory Bulb / Q000378:metabolism; D009833:Olfactory Pathways / Q000502:physiology; D016760:Proto-Oncogene Proteins c-fos / Q000096:biosynthesis*
D016760:Proto-Oncogene Proteins c-fos