Simultaneous quantitative determination of calcium leucovorin and parabens in lyophilized ampoules by derivative spectroscopy.
A simple and rapid procedure for quantitation of Calcium Leucovorin and parabens simultaneously in lyophilized ampoules formulation by "zero crossing" first-order derivative spectroscopy was developed. The ampoules content was dissolved in a mixture water/ethanol (50/50) and first-derivative spectra were recorded. The absolute values of the derivative at 312 nm for the determination of Calcium Leucovorin and between 244 and 246 nm for the determination of parabens were measured. The method is linear, quantitative and reproducible.
Bertini
J
J
Reparto Ricerche Analitiche A. Menarini Industrie Farmaceutiche Riunite S.R.L., Frenze.
Cocchini
A
A
Lamonaca
F
F
Noferini
R
R
Perico
A
A
D002851:Chromatography, High Pressure Liquid; D005612:Freeze Drying; D007202:Indicators and Reagents; D002955:Leucovorin / Q000032:analysis*; D010226:Parabens / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet
D007202:Indicators and Reagents; D010226:Parabens; D002955:Leucovorin