A modified method for experimental candidosis in mice avoiding lethality.
A model is presented which selected one out of 150 Candida albicans strains for the evaluation of antifungal agents. The mice were inoculated with 6 x 10(5) CFU of strain 352 into the tail vein. The strain has a moderate phospholipase B (PLB) activity in vitro and was originally isolated from a stool sample from a patient in an intensive care unit. This infection leads to very little suffering in the infected animals during the 6-day observation period. Kidney counts at day 5 after infection can give a first indication for a possible fungistatic mechanism. Possible interesting drugs can then be evaluated by a second set of experiments using a longer observation time to investigate the compounds for fungicidal properties. The model suggests that screening for systemic antifungals by avoiding lethality of mice in the first place can be done.
Hänel
H
H
Hoechst AG, Frankfurt/Main-Höchst, Germany.
Raether
W
W
Uphoff
M
M
Braun
B
B
Kalisch
J
J
Pastowsky
S
S
D000818:Animals; D002176:Candida albicans / Q000472:pathogenicity*; D002177:Candidiasis / Q000382:microbiology*; D004195:Disease Models, Animal; D007674:Kidney Diseases / Q000382:microbiology; D051379:Mice