title: Immunohistochemical and ultrastructural investigation of granular cell tumours in dog, cat, and horse.
abstract: Six canine, one feline and one equine granular cell tumours (GCTs) were investigated electron microscopically and immunohistochemically. The tumours were tested for reactivity with monoclonal antibodies against vimentin and desmin and with polyclonal antibodies against cytokeratin, S-100 protein, glial fibrillary acidic protein (GFAP) and neuron specific enolase (NSE). All GCTs were characterized by their PAS positive cytoplasmic granules in light microscopy, which in electron microscopy appeared as lysosome-like granules. In each case two canine GCTs were stained by the antibody against cytokeratin, vimentin and S-100 protein. Cells of the equine GCT showed reactivity with the antiserum against S-100 protein. In the feline GCT no reactivity with any of the antibodies tested was observed. These differences of the immunohistochemical reactions of GCTs suggest a nonuniform histogenesis of GCTs in domestic animals. The reactivity of the tumour cells with the antiserum against NSE is discussed.
authors.affiliation: Institut für Tierpathologie, Ludwig-Maximilians-Universität München.
authors.forename: C
authors.initials: C
authors.lastname: Geyer
authors.forename: A
authors.initials: A
authors.lastname: Hafner
authors.forename: S
authors.initials: S
authors.lastname: Pfleghaar
authors.forename: W
authors.initials: W
authors.lastname: Hermanns
mesh_terms: D000818:Animals; D002371:Cat Diseases / Q000473:pathology*; D002415:Cats; D004283:Dog Diseases / Q000473:pathology*; D004285:Dogs; D016586:Granular Cell Tumor / Q000737:chemistry / Q000648:ultrastructure / Q000662:veterinary*; D006734:Horse Diseases / Q000473:pathology*; D006736:Horses; D007150:Immunohistochemistry; D008854:Microscopy, Electron