Anti-HBs detection: a modified passive haemagglutination assay.
Modification of a commercial haemagglutination assay for the detection of anti-HBs has reduced the test time from over one hour to approximately 25 min. increased sensitivity ten-fold without any prozoning, maintained specificity and reduced costs by 90%. The modification consists of diluting the reagent cells ten-fold; these are then added to dilutions of test serum in a V-well microplate. After incubation, plates are centrifuged and then inclined at 70 degrees. Positive and negative reactions can be clearly distinguished within approximately 10 minutes.
McDonald
C P
CP
North London Blood Transfusion Centre, Colindale, UK.
Barbara
J A
JA
al-Izzi
A
A
Ahya
R
R
Vyas
N
N
Contreras
M
M
D000818:Animals; D002645:Chickens; D004912:Erythrocytes; D006386:Hemagglutination Tests / Q000379:methods; D006514:Hepatitis B Surface Antigens / Q000032:analysis*; D013997:Time Factors
D006514:Hepatitis B Surface Antigens