title: Can chromatin texture predict structural karyotypic changes in diploid cells from thyroid cold nodules?
abstract: To assess the effect of a single chromosomal translocation on the nuclear phenotype of human cells, seven diploid adenomas and five diploid carcinomas of the thyroid gland were studied using quantitative nuclear morphometry. Four adenomas and three carcinomas were cytogenetically normal, whereas three adenomas and two carcinomas had a unique chromosomal translocation. A densitometric parameter discriminated adenomas from carcinomas (skewness of the optical density histogram, SODH), and tumours with and without chromosomal translocation (standard deviation of the optical density, SDODH). These results demonstrate that single chromosomal structural rearrangements produce quantifiable alterations of nuclear organisation, but that other nuclear features which do not express an aneuploid DNA content or an abnormal karyotype differentially characterise benign and malignant conditions.
authors.affiliation: Institut Jean-Godinot, Department of Biology, Reims, France.
authors.forename: F
authors.initials: F
authors.lastname: Liautaud-Roger
authors.forename: J R
authors.initials: JR
authors.lastname: Teyssier
authors.forename: D
authors.initials: D
authors.lastname: Ferre
authors.forename: J
authors.initials: J
authors.lastname: Dufer
authors.forename: P
authors.initials: P
authors.lastname: Coninx
mesh_terms: D000236:Adenoma / Q000648:ultrastructure*; D000328:Adult; D002277:Carcinoma / Q000648:ultrastructure*; D002843:Chromatin / Q000648:ultrastructure*; D003720:Densitometry; D003937:Diagnosis, Differential; D005260:Female; D006801:Humans; D007621:Karyotyping / Q000379:methods*; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness / Q000648:ultrastructure; D011003:Ploidies; D013964:Thyroid Neoplasms / Q000648:ultrastructure*; D014178:Translocation, Genetic
chemical_list: D002843:Chromatin