[Serum myoglobin: evaluation of two immunolatex methods. Comparison with a radioimmunoassay method].
Latex immunoturbidimetric (ITA) and immunonephelemetric (INA) assays, were evaluated and compared to a radioimmunological assay (RIA) for the determination of serum myoglobin. Within-run and between-run assays and accuracy and linearity studies gave satisfactory results, very similar to those of RIA. Results were closely correlated with those of RIA for 100 samples of up to 2,000 micrograms/l (correlation coefficients respectively 0.96 and 0.97 for ITA and INA). Reference values (150 men and 150 women; range 20-65 years) were determined and expressed in percentiles. It is concluded that the two latex immunologic assays facilitate laboratory myoglobin evaluation (no radioactive material, short performance time). ITA is more suitable for emergency analysis and INA is more convenient for serial analysis and particular samples (presence of hemoglobin or chylomicrons).
Chivot
L
L
Laboratoire de biochimie A, CHU Hôtel-Dieu, Nantes, France.
Maillot-Vest
M
M
Mainard
F
F
Pare
J M
JM
Massoubre
C
C
Madec
Y
Y
D000328:Adult; D000368:Aged; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009203:Myocardial Infarction / Q000097:blood; D009211:Myoglobin / Q000097:blood*; D009391:Nephelometry and Turbidimetry / Q000379:methods*; D011863:Radioimmunoassay
D009211:Myoglobin
Myoglobine sérique: évaluation de deux méthodes aux immunolatex. Comparaison avec une méthode radio-immunologique.