title: Evaluation of thrombus detection in a rabbit model using a technetium-99m-labelled anti-fibrin monoclonal antibody.
abstract: Technetium-99 m (99mTc)-labelled conjugates of an anti-fibrin monoclonal antibody, DD-3B6/22, have been assessed for their detection of vascular thrombi in a rabbit model. DD-3B6/22 binds to a D-dimer epitope present on cross-linked fibrin but absent from the fibrin monomer or fibrinogen. Injection of a 99mTc-labelled Fab' fragment of DD-3B6/22 allowed delineation of model thrombi as early as 30 min postinjection (p.i.) with optimal localization at 4-5 h. Thrombus label uptake at 4 h p.i. was 0.304 +/- 0.106% injected dose/g (% ID/g) compared with 0.022 +/- 0.001% ID/g after the injection of a control Fab' fragment. These results suggest that the 99mTc-labelled Fab' fragment of DD-3B6/22 has excellent potential for scintigraphic detection of vascular thrombi in humans.
authors.affiliation: ANSTO, Sydney, New South Wales, Australia.
authors.forename: F T
authors.initials: FT
authors.lastname: Lee
authors.forename: L J
authors.initials: LJ
authors.lastname: Milner
authors.forename: G R
authors.initials: GR
authors.lastname: Boniface
authors.forename: G J
authors.initials: GJ
authors.lastname: Bautovich
authors.forename: A R
authors.initials: AR
authors.lastname: Weedon
authors.forename: P G
authors.initials: PG
authors.lastname: Bundesen
authors.forename: D B
authors.initials: DB
authors.lastname: Rylatt
authors.forename: K Z
authors.initials: KZ
authors.lastname: Walker
mesh_terms: D000818:Animals; D000918:Antibody Specificity; D005069:Evaluation Studies as Topic; D005260:Female; D005338:Fibrin Fibrinogen Degradation Products / Q000276:immunology*; D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G / Q000276:immunology; D008297:Male; D011817:Rabbits; D016719:Radioimmunodetection; D013667:Technetium / Q000493:pharmacokinetics; D013927:Thrombosis / Q000000981:diagnostic imaging*; D014018:Tissue Distribution
chemical_list: D005338:Fibrin Fibrinogen Degradation Products; D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G; C036309:fibrin fragment D; D013667:Technetium