Effects of exogenous GM1 ganglioside on LTP in rat hippocampal slices perfused with different concentrations of calcium.
Combined actions of GM1 ganglioside molecules and Ca2+ ions on long-term potentiation (LTP) of the hippocampal CA1 synapses were examined in slice preparations. Application of exogenous GM1 ganglioside significantly enhanced LTP. Antigenic blocking of endogenous GM1 ganglioside by applying anti-GM1 antiserum appeared to attenuate LTP. Exogenous GM1 treatment not only antagonized LTP suppression caused by low Ca2+ (1.0 microM) in the perfusion medium, but also amplified LTP at high Ca2+ (5.0 microM) condition. Thus, GM1 may stabilize intracellular calcium levels to enhance synaptic potentiation.
Hwang
H M
HM
Department of Anatomy, Chang Gung Medical College, Taoyuan, Taiwan, ROC.
Wang
J T
JT
Chiu
T H
TH
D000818:Animals; D002118:Calcium / Q000494:pharmacology*; D004558:Electric Stimulation; D004594:Electrophysiology; D005677:G(M1) Ganglioside / Q000494:pharmacology*; D006624:Hippocampus / Q000187:drug effects / Q000502:physiology*; D008297:Male; D009994:Osmolar Concentration; D010477:Perfusion; D051381:Rats; D011919:Rats, Inbred Strains
D005677:G(M1) Ganglioside; D002118:Calcium