title: Recycling of glutathione during oxidative stress in erythrocytes of the newborn.
abstract: The ability of erythrocytes from newborn babies and adults to maintain reduced glutathione levels during oxidative stress was studied. In vitro incubation of erythrocytes with H2O2, with or without inactivation of catalase, caused a rapid depletion of reduced glutathione (GSH) and concomitant accumulation of oxidized glutathione followed by recovery of GSH and fall of oxidized glutathione to initial values in all subjects. Inactivation of catalase resulted in a 50% loss of intracellular glutathione (p less than 0.005), a larger maximum GSH depletion (p less than 0.05), and a longer GSH recovery time (p less than 0.005). Erythrocytes from newborn babies showed a smaller maximum GSH depletion (p less than 0.05) and a shorter GSH recovery time (p less than 0.005) compared with those from adults. These differences between the newborn and adult groups persisted after inactivation of catalase. An increase in maximum GSH depletion and GSH recovery time (p less than 0.005) was observed when a lower hematocrit was used for these GSH recovery studies. Effective glutathione recycling in erythrocytes may protect immature tissues of the newborn baby from peroxidative damage.
authors.affiliation: Department of Pediatrics, University Hospital of Leiden, The Netherlands.
authors.forename: P C
authors.initials: PC
authors.lastname: Clahsen
authors.forename: R M
authors.initials: RM
authors.lastname: Moison
authors.forename: C A
authors.initials: CA
authors.lastname: Holtzer
authors.forename: H M
authors.initials: HM
authors.lastname: Berger
mesh_terms: D000328:Adult; D002374:Catalase / Q000097:blood; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism*; D005978:Glutathione / Q000031:analogs & derivatives / Q000097:blood*; D019803:Glutathione Disulfide; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D066298:In Vitro Techniques; D007231:Infant, Newborn / Q000097:blood*; D010084:Oxidation-Reduction; D017382:Reactive Oxygen Species / Q000378:metabolism; D013312:Stress, Physiological / Q000097:blood
chemical_list: D017382:Reactive Oxygen Species; D006861:Hydrogen Peroxide; D002374:Catalase; D005978:Glutathione; D019803:Glutathione Disulfide