Use of synthetic H disaccharides as acceptors for detecting activities of UDP-GalNAc:Fuc alpha 1-->2Gal beta-R alpha 1-->3-N-acetylgalactosaminyltransferase in plasma samples from blood group A subgroups.
Concentrations of blood group A-specified alpha(1-->3)-N-acetylgalactosaminyltransferase (A enzyme) were measured in human plasma of blood groups A1, A2, and A3 by using chemically synthesized H disaccharides and H type 1 and type 2 trisaccharides attached to hydrophobic aglycones as acceptors. When the trisaccharides were used as acceptors, enzyme activities were reduced in samples from A2 and A3. However, the H disaccharides were shown to be good acceptors even for enzymes from A2 and A3, and no significant difference in enzyme concentration was detected in any of the plasma tested.
Yazawa
S
S
Department of Legal Medicine, School of Medicine, Gunma University, Maebashi, Japan.
Takeya
A
A
Hosomi
O
O
Nakajima
T
T
Shimoda
N
N
Ohi
H
H
Tachikawa
T
T
Piscorz
C F
CF
Jain
R K
RK
Matta
K L
KL
D000017:ABO Blood-Group System; D002240:Carbohydrate Sequence; D004187:Disaccharides / Q000737:chemistry / Q000378:metabolism*; D006596:Hexosaminidases / Q000378:metabolism; D006801:Humans; D008745:Methylation; D008969:Molecular Sequence Data; D017350:N-Acetylgalactosaminyltransferases / Q000097:blood*; D014312:Trisaccharides / Q000378:metabolism; D001619:beta-N-Acetylhexosaminidases / Q000378:metabolism
D000017:ABO Blood-Group System; D004187:Disaccharides; D014312:Trisaccharides; D017350:N-Acetylgalactosaminyltransferases; C003742:UDPgalactosamine-galactose acetylgalactosaminyltransferase; D006596:Hexosaminidases; D001619:beta-N-Acetylhexosaminidases