[Preparation and characteristics of protoplasts from various strains of Streptomyces roseoflavus var. roseofungini].
It was shown that the variants of the roseofungin-producing organism, which differ in their differentiation, antibiotic activity, structure and cell wall composition had different sensitivity to the protoplasting factors. The protoplasting increased the population heterogeneity: in strain 1128 it was evident from an increased frequency of the secondary colonies, in variant 1-68, folding of the colonies increased and their consistency become milder, sectorial colonies and colonies with coremia formed. It was in principle possible to transform the protoplasts of S. roseoflavus var. roseofungini by plasmid DNA, which suggests that the roseofungin-producing culture may be useful in genetic engineering.
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D000935:Antifungal Agents / Q000096:biosynthesis*; D002473:Cell Wall / Q000378:metabolism / Q000648:ultrastructure; D015169:Colony Count, Microbial; D003470:Culture Media; D005818:Genetic Engineering / Q000379:methods*; D066298:In Vitro Techniques; D010641:Phenotype; D010957:Plasmids / Q000235:genetics*; D011090:Polyenes / Q000378:metabolism; D011523:Protoplasts / Q000166:cytology* / Q000648:ultrastructure; D013291:Streptococcus / Q000166:cytology* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism
D000935:Antifungal Agents; D003470:Culture Media; D011090:Polyenes; C009192:roseofungin
Poluchenie i kharakteristika protoplastov razlichnykh shtammov Streptomyces roseoflavus var. roseofungini.