GRAMP 100: a membrane protein concentrated on secretory granule membranes and the apical cell surface in exocrine acinar cells.
Using a monoclonal antibody (SG10A6) raised against secretion granule membranes of the rat parotid gland, we have identified an antigen that is a common component of both exocrine pancreatic and parotid granule membranes. SG10A6 (an IgM) immunoprecipitates antigen that migrates as a single band (M(r) approximately 80 KD unreduced; M(r) approximately 100 KD reduced) and immunoblots at least two polypeptides that are similar to the reduced and nonreduced immunoprecipitated antigen. This granule-associated membrane polypeptide (GRAMP 100; named for the apparent M(r) in reduced form) is also a prominent component of plasma membrane fractions. Immunocytochemical localization at the electron microscopic level demonstrates the presence of GRAMP 100 on granule membranes, especially condensing vacuoles and exocytotic figures, and the apical plasma membrane. Lower levels of antigen are detected on basolateral plasma membrane and on peri-Golgi membranes that may be part of the endosomal system. Both the cell fractionation and immunocytochemical localization indicate that GRAMP 100 differs in distribution from GRAMP 92 and 30K SCAMPs, two other components of exocrine granule membranes identified with monoclonal antibodies. To date, no polypeptides have been identified with this approach that are exclusive components of exocrine granule membranes.
Laurie
S M
SM
Department of Anatomy and Cell Biology, University of Virginia Health Sciences Center, Charlottesville 22908.
Mixon
M B
MB
Castle
J D
JD
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002462:Cell Membrane / Q000737:chemistry / Q000648:ultrastructure; D003594:Cytoplasmic Granules / Q000737:chemistry / Q000648:ultrastructure*; D006056:Golgi Apparatus / Q000737:chemistry / Q000648:ultrastructure; D015151:Immunoblotting; D007150:Immunohistochemistry; D007425:Intracellular Membranes / Q000737:chemistry* / Q000648:ultrastructure; D008565:Membrane Proteins / Q000032:analysis* / Q000276:immunology; D008854:Microscopy, Electron; D010306:Parotid Gland / Q000166:cytology* / Q000648:ultrastructure; D011233:Precipitin Tests; D051381:Rats
D000911:Antibodies, Monoclonal; D008565:Membrane Proteins