title: Sphincter of Oddi regulates flow by acting as a variable resistor to flow.
abstract: Two models of transsphincteric flow and a model evaluating pumping activity were established in the anesthetized Australian brush-tailed possum to determine whether the sphincter of Oddi (SO) acts as a resistor or as a pump. A simple model of transsphincteric flow (inflow only) demonstrated that at physiological common bile duct (CBD) pressure, 9.5 +/- 0.3 cmH2O (n = 7), transsphincteric flow occurred between SO pressure waves (n = 10). A second more complex transsphincteric flow model was established that permitted simultaneous measurements of inflow, outflow, CBD pressure, SO basal pressure, SO contraction frequency, and amplitude. At physiological CBD pressure, inflow always equaled outflow (157.0 +/- 11.2 and 156.4 +/- 11.4 microliters/min, respectively; n = 7). The SO displayed regular contractions superimposed on a basal pressure of 1.1 +/- 0.4 mmHg. Contraction amplitude was 12.6 +/- 3.0 mmHg and the frequency was 3.6 +/- 0.4 contractions/min (n = 7). Pressure waves recorded in the CBD corresponded to the SO contractions and reflected SO activity. Transsphincteric flow occurred between SO contractions and was obstructed by these contractions. Stimulation of SO activity (basal pressure and contraction frequency) with intra-arterial injections of motilin (200 ng/kg) or erythromycin (200 micrograms/kg) abolished transsphincteric flow. Reduction in SO contraction frequency to 72.7 +/- 7.2% (P < 0.01, paired t test) after administration of Cisapride (2 mg/kg iv) increased transsphincteric flow to 147.6 +/- 12.3% (n = 7, P < 0.05, paired t test). In six possums, possible SO pumping action was evaluated. A manometer was connected to the CBD, and a second manometer was connected to the duodenum surrounding the papilla.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Surgery, Flinders University, Australia.
authors.forename: Y F
authors.initials: YF
authors.lastname: Liu
authors.forename: G T
authors.initials: GT
authors.lastname: Saccone
authors.forename: A
authors.initials: A
authors.lastname: Thune
authors.forename: R A
authors.initials: RA
authors.lastname: Baker
authors.forename: J R
authors.initials: JR
authors.lastname: Harvey
authors.forename: J
authors.initials: J
authors.lastname: Toouli
mesh_terms: D000818:Animals; D001646:Bile / Q000187:drug effects / Q000502:physiology*; D020117:Cisapride; D004917:Erythromycin / Q000494:pharmacology; D005260:Female; D008297:Male; D009037:Motilin / Q000494:pharmacology; D009893:Opossums; D010880:Piperidines / Q000494:pharmacology; D011312:Pressure; D009803:Sphincter of Oddi / Q000187:drug effects / Q000502:physiology*
chemical_list: D010880:Piperidines; D009037:Motilin; D004917:Erythromycin; D020117:Cisapride