Neurotrophic effects of epidermal growth factor on cultured brain neurons are blocked by protein kinase inhibitors.
The influences of protein kinase inhibitors, K-252a and staurosporine, on the neurotrophic effects of epidermal growth factor (EGF) were investigated in dissociated cell cultures of the hippocampus and cerebellum of fetal rats. Addition of 1 ng/ml EGF enhanced the survival of cultured neurons of both brain regions. Both K-252a (10-200 nM) and staurosporine (1-100 nM) blocked the survival-promoting effects of EGF in a concentration-dependent manner. These results suggest that activation of protein kinase(s) is involved in the neurotrophic effects of EGF.
Abe
K
K
Department of Chemical Pharmacology, Faculty of Pharmaceutical Sciences, University of Tokyo, Japan.
Takayanagi
M
M
Saito
H
H
D000470:Alkaloids / Q000494:pharmacology; D000818:Animals; D001921:Brain / Q000166:cytology / Q000187:drug effects*; D002227:Carbazoles / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004815:Epidermal Growth Factor / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D026121:Indole Alkaloids; D009474:Neurons / Q000166:cytology / Q000187:drug effects; D011493:Protein Kinase C / Q000037:antagonists & inhibitors*; D051381:Rats; D011994:Recombinant Proteins / Q000037:antagonists & inhibitors / Q000494:pharmacology; D019311:Staurosporine
D000470:Alkaloids; D002227:Carbazoles; D026121:Indole Alkaloids; D011994:Recombinant Proteins; D004815:Epidermal Growth Factor; C049985:staurosporine aglycone; D011493:Protein Kinase C; D019311:Staurosporine