[Detection of dystrophin with anti-peptide antibodies].
The analysis of dystrophin in skeletal muscles was performed to identify Duchenne and Becker muscular dystrophy (DMD and BMD) by means of immunohistochemical stain and Western blotting with antisera against synthetic dystrophin peptides. The control muscle specimens derived from normal healthy persons, and patients without DMD and BMD revealed clearly continuous stains of dystrophin at surface membrane. A band with 400 kDa of molecular size by Western blotting was positively stained by anti-dystrophin antibodies. The muscle specimens from eleven DMD patients showed no observation both in the band on Western blotting and in the immunohistochemical staining of dystrophin on frozen-thin sections. BMD muscle specimens showed patchy and faint stains, but no detection of any band on Western blotting except a 380 kDa minor band with anti-peptide IX antibody in one patient muscle. The immunohistochemical procedure was found to be more sensitive than Western blotting for the detection of dystrophin. These results indicate that the dystrophin analysis by both methods is an useful tool for the differential diagnosis of patients with DMD and BMD.
Takeda
A
A
Department of Clinical Pathology, Showa University Fujigaoka Hospital, Yokohama.
Jimi
T
T
Misugi
N
N
Miyake
S
S
Kumagai
T
T
Nakamura
Y
Y
Wakayama
Y
Y
D000818:Animals; D015153:Blotting, Western; D003937:Diagnosis, Differential; D016189:Dystrophin / Q000032:analysis* / Q000276:immunology; D006801:Humans; D007106:Immune Sera; D007150:Immunohistochemistry; D009132:Muscles / Q000737:chemistry; D009136:Muscular Dystrophies / Q000175:diagnosis; D010455:Peptides / Q000276:immunology; D011817:Rabbits
D016189:Dystrophin; D007106:Immune Sera; D010455:Peptides