Identification of metabolites of halofantrine, a new candidate anti-malarial drug, by gas chromatography-mass spectrometry.
Two previously unknown metabolites of halofantrine, a candidate anti-malarial drug, have been isolated by thin-layer chromatography from the plasma of dogs administered a single oral dose of 60 mg/kg. Their identifies were investigated after trimethylsilylation by gas chromatography-mass spectrometry under electron-impact and negative-ion chemical ionization conditions. The structural assignment was further confirmed by using a combination of elemental composition analysis of all the isotope peaks at low mass resolution and isotope pattern matching. These two metabolites were formed by modification of the dibutylaminopropyl side-chain of the parent compound involving deamination and oxidation or reduction.
Cheng
K N
KN
Department of Metabolism and Radiosynthesis, Huntingdon Research Centre, Ltd., Cambridgeshire, UK.
Elsom
L F
LF
Hawkins
D R
DR
D000818:Animals; D000962:Antimalarials / Q000737:chemistry* / Q000378:metabolism; D002855:Chromatography, Thin Layer; D004285:Dogs; D008401:Gas Chromatography-Mass Spectrometry; D008297:Male; D010616:Phenanthrenes / Q000737:chemistry* / Q000378:metabolism
D000962:Antimalarials; D010616:Phenanthrenes; C023768:halofantrine