Substrate preference of metalloproteinases secreted by ts-110 Moloney murine sarcoma virus-transformed normal rat kidney cells.
In this study, the two forms of affinity-purified transformation-associated proteins (TAPs) (68 and 64 kD) were shown to have different substrate preferences. For the 68-kD TAP, the order of substrate preference was collagen types I, III, and V; fibronectin; gelatin; and collagen IV. For the 64-kD TAP, the order of substrate preference was collagen I, III, and V and gelatin. The 64-kD TAP did not cleave collagen IV and fibronectin. We also found a 71-kD metalloproteinase in the concentrated purified TAPs that reacted only weakly with a TAP monoclonal antibody and showed this substrate preference: collagen I, III, and V; gelatin; and collagen IV. Whether this 71-kD TAP is a new member of the rat metalloproteinase family will be investigated.
Zhang
H Z
HZ
Department of Tumor Biology, University of Texas M. D. Anderson Cancer Center, Houston 77030.
Scanlon
M
M
Murray
J L
JL
Chan
J C
JC
D000818:Animals; D000906:Antibodies; D000911:Antibodies, Monoclonal; D015153:Blotting, Western; D002461:Cell Line, Transformed; D002471:Cell Transformation, Neoplastic; D003094:Collagen / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D007668:Kidney; D008666:Metalloendopeptidases / Q000302:isolation & purification / Q000378:metabolism*; D008970:Molecular Weight; D008980:Moloney murine sarcoma virus / Q000235:genetics*; D051381:Rats; D013379:Substrate Specificity
D000906:Antibodies; D000911:Antibodies, Monoclonal; D003094:Collagen; D008666:Metalloendopeptidases