Heparin stimulates the collagen synthesis in mineralized cultures of the osteoblast-like cell line, MC3T3-E1.
We found that heparin induced an increase in collagen synthesis in MC3T3-E1 cells cultured for 15 and 30 days. Northern blots showed that the effect of heparin on the collagen synthesis was mediated through the mRNA expression of type I collagen (day 15). Although heparin stimulated collagen synthesis over and above that stimulated by the transforming growth factor (TGF)-beta, heparin did not stimulate TGF-beta binding. This study indicates that heparin has special tasks both in bone formation and resorption, since it has the ability to form and degrade collagen. We suggest that heparin assists the regulation of collagen metabolism at bone resorption sites.
Shibata
Y
Y
Department of Biochemistry, Nihon University School of Dentistry at Matsudo, Chiba, Japan.
Abiko
Y
Y
Goto
K
K
Moriya
Y
Y
Takiguchi
H
H
D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D015342:DNA Probes; D015870:Gene Expression / Q000187:drug effects; D006493:Heparin / Q000494:pharmacology*; D051379:Mice; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010006:Osteoblasts / Q000187:drug effects* / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000378:metabolism / Q000494:pharmacology
D015342:DNA Probes; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta; D006493:Heparin; D003094:Collagen