Specific interactions between the IclR repressor of the acetate operon of Escherichia coli and its operator.
The positions of interference points between the IclR repressor of the acetate operon of Escherichia coli and its specific operator were examined. The number and nature of nucleotides essential to repressor binding were determined by scanning populations of DNA previously methylated at guanine residues by dimethyl sulfate, or depurinated by treatment with formic acid, or depyrimidated by treatment with hydrazine. A total of 46 nucleotides, distributed almost equally between the two strands of the operator region, were found to be functionally important, although to a varying extent. These are clustered in two successive domains which expand from nucleotide -54 to nucleotide -27 and can organize in a palindrome-like structure containing a large proportion of A and T residues.
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Institute of Biology and Chemistry of Proteins, C.N.R.S., University of Lyon, Villeurbanne, France.
Cortay
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JC
Galinier
A
A
Sauve
P
P
Cozzone
A J
AJ
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000378:metabolism*; D001483:Base Sequence; D001665:Binding Sites; D004269:DNA, Bacterial; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins; D008745:Methylation; D008969:Molecular Sequence Data; D009841:Oligonucleotides; D009875:Operator Regions, Genetic; D009876:Operon; D016133:Polymerase Chain Reaction; D012097:Repressor Proteins / Q000378:metabolism*; D014157:Transcription Factors
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; D009841:Oligonucleotides; D012097:Repressor Proteins; D014157:Transcription Factors; C067479:IclR protein, E coli