[Evaluation of Oriton IgE, a new kit for measurement of allergen specific IgE antibodies].
To determine whether Oriton IgE kit, a new kit for the measurement of allergen-specific IgE antibodies, is useful in screening allergen-specific antibody, we measured the titers of IgE antibodies against 11 different allergens (house dust 2, Dermatophagoides farinae, Japanese cedar, ragweed pollen, egg white, milk, cat epithelium, dog epithelium, Candida, Alternaria and Aspergillus) with the Oriton IgE kit, and the results were compared to those of intradermal tests and RAST in 103 allergic patients and 10 normal subjects. There was a clear correlation between IgE antibody titers measured by the Oriton IgE kit and the RAST. The correlation coefficient was 0.76 (p less than 0.01) and the total correspondence rate was 85.9%. We also found strong correlation between the Oriton IgE kit and RAST in IgE antibody titer against 5 different allergens, Dermatophagoides farinae, Japanese cedar, ragweed pollen, egg white and Candida. The correlation coefficient was over 0.70. The correspondence rate, sensitivity and specificity of the Oriton IgE kit to intradermal tests was 71.8%, 45.3% and 87.8% respectively. The sensitivity of the Oriton IgE kit was slightly higher, while the specificity was slightly higher in RAST, although the differences were not statistically significant between these methods. Correspondence rate of the Oriton IgE kit was similar to that of RAST. These results suggested that the Oriton IgE kit is useful in screening allergen specific IgE antibodies.
Suganuma
T
T
Department of Kawakami Memorial Medical Centure for Allergy.
Kawakami
Y
Y
Adachi
M
M
Takahashi
T
T
Baba
M
M
Iwasaki
E
E
Miyabayashi
Y
Y
D000485:Allergens / Q000276:immunology*; D005069:Evaluation Studies as Topic; D006801:Humans; D007073:Immunoglobulin E / Q000032:analysis* / Q000276:immunology; D011933:Reagent Kits, Diagnostic
D000485:Allergens; D011933:Reagent Kits, Diagnostic; D007073:Immunoglobulin E