Diversity of human anti-D monoclonal antibodies revealed by reactions with chimpanzee red blood cells.
Fifty-three human anti-D monoclonal antibodies (mAbs) revealed a striking diversity of reactions in tests with panels of chimpanzee red blood cells (RBCs) of various R-C-E-F blood group phenotypes (counterparts of the human Rh-Hr groups). The reactivities of these antibodies, which depended on the agglutination technique used, could be classified into four main types. These patterns of reactivity of anti-D mAbs with chimpanzee RBCs showed only limited correlation with types of reactions observed with human D variant RBCs. Primate red cells may, therefore, constitute an independent test system for subclassification of human monoclonal antibodies. Comparison of reactivities of human anti-D mAbs with chimpanzee and human D variant RBCs confirms the homology between the chimpanzee Rc, and the human D antigens. The chimpanzee Rc shares with human D the epitopes epD5, epD6/7 and epD8, but lacks epitopes epD1, epD2, epD3 and epD4 of the Rh mosaic, thus resembling the human D variants IVb and Vc.
Blancher
A
A
Centre Régional de Transfusion Sanguine, CHU Purpan, Toulouse, France.
Socha
W W
WW
Ruffie
J
J
D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry*; D004912:Erythrocytes / Q000276:immunology*; D014644:Genetic Variation; D006801:Humans; D016130:Immunophenotyping; D007202:Indicators and Reagents; D002679:Pan troglodytes / Q000097:blood; D012204:Rh-Hr Blood-Group System / Q000276:immunology*
D000911:Antibodies, Monoclonal; D007202:Indicators and Reagents; D012204:Rh-Hr Blood-Group System