Identification of Leishmania donovani isolates from different kala-azar foci in China by kDNA hybridization.
kDNA sequence homology of Leishmania donovani isolates from three types of kala-azar foci in China were analyzed by using dot and Southern hybridization with biotin- and 32P-labelled probes. The results revealed kDNA sequence heterogeneity among Leishmania donovani isolates from the three kala-azar foci: sequence homology between isolates of hill and desert foci was higher than that between hill and plain foci isolates. The kDNA hybridization technique was also found to be specific and sensitive for direct identification of Leishmania in animal tissues. In a preliminary survey, kDNA hybridization of cutaneous tissue blots of 71 dogs from endemic regions showed a positive rate of 40.8%, and the rate of double positive cases (touch blot hybridization and bone marrow smear) reached 91.3%. The direct identification of Leishmania in tissues by kDNA hybridization seems to be a useful and convenient method for epidemiological study and clinical diagnosis, especially for species/strain characterization.
Hu
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Laboratory of Parasitology, West China University of Medical Sciences, Chengdu.
Lu
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Luo
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Wang
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Hu
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Yi
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D000818:Animals; D015139:Blotting, Southern; D015342:DNA Probes; D016054:DNA, Protozoan / Q000032:analysis / Q000235:genetics*; D004285:Dogs; D006801:Humans; D007893:Leishmania donovani / Q000235:genetics / Q000302:isolation & purification*; D007898:Leishmaniasis, Visceral / Q000469:parasitology* / Q000662:veterinary; D009693:Nucleic Acid Hybridization; D012689:Sequence Homology, Nucleic Acid; D012867:Skin / Q000469:parasitology; D013045:Species Specificity
D015342:DNA Probes; D016054:DNA, Protozoan