DOT-ELISA for detection of phenolic glycolipid PGL-Tb1 and diacyl-trehalose antigens of Mycobacterium tuberculosis.
A DOT-ELISA method for detection of 2,3-diacyl-trehalose (DAT, previously referred to as SL-IV antigen) and triglycosyl phenol phthiocerol di-mycocerosate (PGL-Tb1) antigens from Mycobacterium tuberculosis is described. The method enabled the detection of both antigens in 14 clinical isolates of M. tuberculosis from different geographic origins; the presence of the glycolipids was confirmed by chemical analysis. It was therefore concluded that the synthesis of both of these compounds is characteristic of the species.
Papa
F
F
Unité de la Tuberculose et des Mycobactéries, Institut Pasteur, Paris.
Luquin
M
M
David
H L
HL
D000942:Antigens, Bacterial; D004797:Enzyme-Linked Immunosorbent Assay; D006017:Glycolipids / Q000032:analysis* / Q000276:immunology; D006801:Humans; D015151:Immunoblotting / Q000379:methods*; D066298:In Vitro Techniques; D009169:Mycobacterium tuberculosis / Q000737:chemistry* / Q000276:immunology
D000942:Antigens, Bacterial; D006017:Glycolipids; C045169:phenolic glycolipid I, Mycobacterium leprae