title: Determination of proteinase 3-alpha 1-antitrypsin complexes in inflammatory fluids.
abstract: Physiological inhibitors were tested for their in vitro interaction with neutrophil proteinase 3 (PR3). The major plasma proteinase inhibitor of PR3 is alpha 1AT. We have developed a radioimmunoassay (RIA) for quantitative detection of PR3-alpha 1AT complexes formed in vivo in inflammatory exudates such as synovial fluid and plasma from patients with sepsis. Levels of PR3-alpha 1AT complexes correlated significantly with levels of human neutrophil elastase (HNE)-alpha 1AT complexes. Thus, in vivo alpha 1AT not only protects against excessive HNE activity, but also against excessive PR3 activity.
authors.affiliation: Central Laboratory The Netherlands Red Cross Blood Transfusion Service Amsterdam, Amsterdam.
authors.forename: K M
authors.initials: KM
authors.lastname: Dolman
authors.forename: B A
authors.initials: BA
authors.lastname: van de Wiel
authors.forename: C M
authors.initials: CM
authors.lastname: Kam
authors.forename: J J
authors.initials: JJ
authors.lastname: Abbink
authors.forename: C E
authors.initials: CE
authors.lastname: Hack
authors.forename: A
authors.initials: A
authors.lastname: Sonnenberg
authors.forename: J C
authors.initials: JC
authors.lastname: Powers
authors.forename: A E
authors.initials: AE
authors.lastname: von dem Borne
authors.forename: R
authors.initials: R
authors.lastname: Goldschmeding
mesh_terms: D001172:Arthritis, Rheumatoid / Q000378:metabolism; D016470:Bacteremia / Q000378:metabolism; D006801:Humans; D007249:Inflammation / Q000378:metabolism*; D053146:Myeloblastin; D011487:Protein Conformation; D011863:Radioimmunoassay / Q000379:methods; D012697:Serine Endopeptidases / Q000097:blood / Q000378:metabolism*; D000515:alpha 1-Antitrypsin / Q000032:analysis / Q000378:metabolism*
chemical_list: D000515:alpha 1-Antitrypsin; D012697:Serine Endopeptidases; D053146:Myeloblastin