[Studies on the analytical method of a new anti-arrhythmic guan-fu base A in rabbit plasma].
Guan-fu base A, a new antiarrhythmic alkaloid with a structure of C20-diterpenoid, was first isolated from the tuber of Aconitum coreanum (Levl.) Raipaics in China in 1966. An analytical method of GC-ECD has been established in this paper for the determination of its concentration in rabbit plasma in order to study its pharmacokinetics in preclinical pharmacology. Guan-fu base A and the internal standard alprenolol (ALP) was found to react rapidly and quantitatively with trifluoroacetic anhydride (TFAA) to produce derivatives GFA-TFAA and ALP-TFAA which were identified by GC-MSD. An optimal GC-ECD system for the separation and determination of the derivatives following the sample preparation was established. There was a wide linear range of the method from 10-2 x 10(4) ng/ml (r = 0.9984). The average recovery of Guan-fu base A from the spiked plasma was 97.52%. The coefficient of variation of between-day and within-day were less than 9% and 7%, respectively. The method was used to determine Guan-fu base A in the plasma of 15 rabbits following intravenous administration of 3 dosages of 0.4, 2 and 10 mg per kg of body weight.
Yang
L L
LL
Department of Instrumental Analysis, Jinling Hospital, Nanjing.
Yang
X J
XJ
Liu
J H
JH
Li
N S
NS
Qiu
N Y
NY
D000470:Alkaloids / Q000097:blood*; D000818:Animals; D000889:Anti-Arrhythmia Agents / Q000097:blood*; D002849:Chromatography, Gas / Q000379:methods; D006576:Heterocyclic Compounds, 4 or More Rings; D011817:Rabbits
D000470:Alkaloids; D000889:Anti-Arrhythmia Agents; D006576:Heterocyclic Compounds, 4 or More Rings; C040615:Guan-fu base A