Truncated pre-S/S proteins transactivate multiple target sequences.
In order to investigate the transactivational function of HBV truncated preS/S proteins we have constructed two sets of plasmids and have tested their transactivational potential on the c-myc regulatory sequences and the TPA-responsive element. We found that preS/S proteins only become transactivationally active when truncated at the carboxy terminal end. Furthermore, using immunofluorescence microscopy we determined that the proteins are located exclusively in the cytoplasm, apparently ruling out DNA binding and activation of factors in the nucleus.
Natoli
G
G
I Clinica Medica, Fondazione Andrea Cesalpino Policlinico Umberto I, Rome, Italy.
Balsano
C
C
Avantaggiati
M L
ML
De Marzio
E
E
Artini
M
M
Collepardo
D
D
Elfassi
E
E
Levrero
M
M
D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D004252:DNA Mutational Analysis; D016259:Genes, myc / Q000187:drug effects / Q000235:genetics*; D006514:Hepatitis B Surface Antigens / Q000235:genetics* / Q000302:isolation & purification / Q000494:pharmacology; D006515:Hepatitis B virus / Q000235:genetics*; D011498:Protein Precursors / Q000302:isolation & purification / Q000494:pharmacology; D011993:Recombinant Fusion Proteins / Q000235:genetics; D012045:Regulatory Sequences, Nucleic Acid / Q000187:drug effects / Q000235:genetics*; D013329:Structure-Activity Relationship; D015533:Transcriptional Activation
D006514:Hepatitis B Surface Antigens; D011498:Protein Precursors; D011993:Recombinant Fusion Proteins; D015500:Chloramphenicol O-Acetyltransferase