title: A strategy for production of monoclonal antibodies to Echinococcus granulosus antigen 5 and antigen B.
abstract: Serum antibody responses to sheep hydatid cyst fluid (SHCF) and a purified Antigen 5 (Ag5) were examined in ELISA, immunoelectrophoresis (IEP) and immunoprecipitation (IP) to facilitate production of monoclonal antibodies (MAb) to E. granulosus Ag5 and Antigen B (AgB). Although sera from mice immunized with SHCF contained antibodies of various classes, the fusions using these donor mice resulted in mainly anti-AgB MAb, possibly due to the preferential selection of MAb to AgB by the SHCF-based ELISA screening system. Donor mice immunized with Ag5 also produced several classes of antibodies, and the resultant fusions enabled selection of IgG MAb to Ag5.
authors.affiliation: University of Melbourne, Veterinary Clinical Centre, Werribee, Victoria, Australia.
authors.forename: D
authors.initials: D
authors.lastname: Liu
authors.forename: M D
authors.initials: MD
authors.lastname: Rickard
authors.forename: M W
authors.initials: MW
authors.lastname: Lightowlers
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000096:biosynthesis*; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D000947:Antigens, Helminth / Q000276:immunology*; D004446:Echinococcus / Q000276:immunology*; D005260:Female; D006825:Hybridomas; D008297:Male; D051379:Mice; D012756:Sheep
chemical_list: D000909:Antibodies, Helminth; D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth