A distal region enhances the prolactin induced promoter activity of the rabbit alpha s1-casein gene.
Casein gene expression is induced in the rabbit mammary gland by prolactin (PRL). alpha s1-casein is the major casein secreted into milk. In order to define the position of the DNA sequences involved in the control of rabbit alpha s1-casein gene regulation by PRL, chimeric genes were constructed between upstream regions of the rabbit alpha s1-casein gene and the chloramphenicol acetyl transferase (CAT) reporter gene. A series of 5'-deleted fusion genes was obtained by nuclease digestion of the alpha s1-casein gene upstream region. These gene constructs were transfected into rabbit primary mammary cells, or cotransfected in CHO cells with the plasmid coding for the rabbit mammary receptor (PRL-R). A regulatory region has been located between nt -3768 and -3155. This region enhances the prolactin induced promoter activity of the alpha s1-casein gene. It might possess or cooperate with prolactin responsive elements located further downstream in the alpha s1-casein gene.
Pierre
S
S
Unité de Différenciation Cellulaire, Institut National de la Recherche Agronomique, Jouy-en-Josas, France.
Jolivet
G
G
Devinoy
E
E
Théron
M C
MC
Maliénou-N'Gassa
R
R
Puissant
C
C
Houdebine
L M
LM
D000818:Animals; D016466:CHO Cells; D002364:Caseins / Q000096:biosynthesis / Q000235:genetics*; D002478:Cells, Cultured; D006224:Cricetinae; D004742:Enhancer Elements, Genetic; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects*; D005813:Genes, Synthetic; D008321:Mammary Glands, Animal / Q000166:cytology; D011247:Pregnancy; D011388:Prolactin / Q000494:pharmacology*; D011401:Promoter Regions, Genetic / Q000187:drug effects*; D011817:Rabbits / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics
D002364:Caseins; D011993:Recombinant Fusion Proteins; D011388:Prolactin