Identification of a 58-kDa antigen with increased immunoreactivity in the cerebella of multiple sclerosis patients.
An increase in immunoreactivity associated with a 58-kDa antigen was found in a majority of MS cerebellar homogenates examined by Western blot analysis using antisera obtained by selective immunization of rabbits with autopsy cerebella. Two-dimensional immunoblotting demonstrated that the majority of the increased immunoreactivity observed in MS cerebella was associated with the highest apparent pI of three immunoreactive species at 58 kDa. Immuno-crossreaction with rat cerebellar homogenates demonstrated that the 58-kDa antigen was developmentally regulated, showing the greatest immunoreactivity at embryonic day 15. The 58-kDa cerebellar antigen may represent a membrane protein which is re-expressed as part of the onset of MS.
Peuchen
S
S
Department of Pathology, Medical College of Virginia, Virginia Commonwealth University, Richmond.
Gruemer
H D
HD
DeVries
G H
GH
D000818:Animals; D000943:Antigens, Differentiation / Q000096:biosynthesis* / Q000276:immunology; D015153:Blotting, Western; D002531:Cerebellum / Q000196:embryology / Q000276:immunology*; D002850:Chromatography, Gel; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D004622:Embryo, Mammalian / Q000276:immunology; D006801:Humans; D009103:Multiple Sclerosis / Q000276:immunology*; D051381:Rats
D000943:Antigens, Differentiation