Poly(ADP-ribose)polymerase-activity of chicken embryo cells exposed to nucleotoxic agents.
Poly(ADP-ribose)polymerase (PARP)-activity was assessed in vitro from the incorporation of the adenosine-diphosphate-ribose moiety of 14C-NAD+ in the acid-insoluble cell fraction. When compared to mammalian (rat) cells, chicken embryo cells exhibit an almost three- to fourfold higher constitutive PARP-activity and an about two- to threefold lower chromatin compactness as evidenced by viscometry of alkaline cell lysates and nucleoid sedimentation. X-irradiation, bleomycin and H2O2 activated PARP. Hyperthermia (43 degrees C), doxorubicin, ethidium bromide and novobiocin resulted in an inhibition of the enzyme activity. Even at the highest doses used, UV-light, monofunctionally alkylating agents and the bisbenzimide Hoechst 33258 remained without significant effects. It is suggested that, with respect to DNA-and/or chromatin-interactive agents, the chicken embryo PARP-test may be complementary to the results of morphological and biochemical studies.
Ignatius
A
A
Institute of Pharmacology, Toxicology and Pharmacy, University of Munich, Germany.
Hund
M
M
Tempel
K
K
D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000201:enzymology / Q000528:radiation effects; D002478:Cells, Cultured; D002642:Chick Embryo; D002843:Chromatin / Q000187:drug effects; D004249:DNA Damage; D008099:Liver / Q000187:drug effects* / Q000201:enzymology / Q000528:radiation effects; D009153:Mutagens / Q000633:toxicity*; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism*; D011865:Radioisotope Dilution Technique; D012680:Sensitivity and Specificity; D013950:Thymus Gland / Q000187:drug effects* / Q000201:enzymology / Q000528:radiation effects
D002843:Chromatin; D009153:Mutagens; D011065:Poly(ADP-ribose) Polymerases