Response of nasal mucosa to histamine or methacholine challenge: use of a quantitative method to examine the modulatory effects of atropine and ipratropium bromide.
We have developed a new technique for the direct local administration of test solutions to the nasal mucosa and for quantification of nasal secretory responses. This technique, a variation on several published reports of filter paper use, allows simple and rapid determination of drug effects and facilitates the analysis of ipsilateral and contralateral responses to local challenge of the nasal mucosa. We have used this technique to investigate the secretory responses of the nasal mucosa to methacholine and histamine and to determine the effects of atropine and ipratropium bromide (Atrovent nasal spray) on these secretory responses.
Naclerio
R M
RM
Johns Hopkins University School of Medicine, Baltimore, MD.
Baroody
F M
FM
D001285:Atropine / Q000008:administration & dosage; D004305:Dose-Response Relationship, Drug; D005374:Filtration / Q000295:instrumentation; D006632:Histamine / Q000008:administration & dosage; D006801:Humans; D009241:Ipratropium / Q000008:administration & dosage; D016210:Methacholine Chloride / Q000008:administration & dosage; D009297:Nasal Mucosa / Q000187:drug effects* / Q000378:metabolism; D009299:Nasal Provocation Tests / Q000295:instrumentation / Q000379:methods; D010209:Paper; D012221:Rhinitis, Allergic, Perennial / Q000175:diagnosis
D016210:Methacholine Chloride; D001285:Atropine; D006632:Histamine; D009241:Ipratropium