title: Anti-HBs detection: a modified passive haemagglutination assay.
abstract: Modification of a commercial haemagglutination assay for the detection of anti-HBs has reduced the test time from over one hour to approximately 25 min. increased sensitivity ten-fold without any prozoning, maintained specificity and reduced costs by 90%. The modification consists of diluting the reagent cells ten-fold; these are then added to dilutions of test serum in a V-well microplate. After incubation, plates are centrifuged and then inclined at 70 degrees. Positive and negative reactions can be clearly distinguished within approximately 10 minutes.
authors.affiliation: North London Blood Transfusion Centre, Colindale, UK.
authors.forename: C P
authors.initials: CP
authors.lastname: McDonald
authors.forename: J A
authors.initials: JA
authors.lastname: Barbara
authors.forename: A
authors.initials: A
authors.lastname: al-Izzi
authors.forename: R
authors.initials: R
authors.lastname: Ahya
authors.forename: N
authors.initials: N
authors.lastname: Vyas
authors.forename: M
authors.initials: M
authors.lastname: Contreras
mesh_terms: D000818:Animals; D002645:Chickens; D004912:Erythrocytes; D006386:Hemagglutination Tests / Q000379:methods; D006514:Hepatitis B Surface Antigens / Q000032:analysis*; D013997:Time Factors
chemical_list: D006514:Hepatitis B Surface Antigens