Permeable collagen-glycosaminoglycan cross-linked copolymers for the study of biological responses of cocultured Sertoli and spermatogenic cells.
A novel collagen-glycosaminoglycan (C-GAG) substrate was developed to overcome the optical opacity of a HATF nitrocellulose substrate and to provide a more physiological permeable substrate for cocultured Sertoli and spermatogenic cells. Cocultures were prepared on optically transparent C-GAG discs attached to a polyester mesh to facilitate handling. Sertoli cells displayed a cuboidal-to-columnar shape; a large number of spermatogonia and primary spermatocytes connected by intercellular bridges were associated with basolateral and apical surfaces of Sertoli cells up to 12 days after plating. Rat Sertoli-spermatogenic cell cocultures have been used for testing the effect of toxicants on rat spermatogenesis in vitro. In our initial studies, we tested the effects of the toxicant gossypol on spermatogenic cells cocultured with Sertoli cells on non-permeable (plastic) and permeable substrates (HATF nitrocellulose) under both standard culture conditions and during perifusion after achieving a continuous electrical-resistant cell monolayer. A selective mitochondrial structural damage was observed in spermatogenic cells (spermatogonia and spermatocytes) but not in the coexisting Sertoli cells. This damage was time- (15-60 min) and dose-dependent (0.1-10 microM) and developed more rapidly under perifusion conditions. Similar mitochondrial damage was reported in the intact animal but required higher concentrations (mg) and longer administration time (months) for detection. Studies are in progress to evaluate the effect of additional toxic chemical agents on functional properties of Sertoli and spermatogenic cells in cocultures prepared on various classes of C-GAG substrates.
Tres
L L
LL
Department of Cell Biology and Anatomical Sciences, City University of New York Medical School, New York 10031.
Cahn
F
F
Kierszenbaum
A L
AL
D000818:Animals; D001704:Biopolymers; D002455:Cell Division / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D003094:Collagen / Q000737:chemistry*; D003101:Collodion; D006025:Glycosaminoglycans / Q000737:chemistry*; D006072:Gossypol / Q000494:pharmacology; D008297:Male; D010539:Permeability; D010969:Plastics; D051381:Rats; D012708:Sertoli Cells / Q000166:cytology* / Q000187:drug effects; D013091:Spermatogenesis / Q000187:drug effects / Q000502:physiology*
D001704:Biopolymers; D006025:Glycosaminoglycans; D010969:Plastics; D003101:Collodion; D003094:Collagen; D006072:Gossypol