title: Cytotoxicity and cytokinetic effects of mitomycin C and/or photochemotherapy in a human colon adenocarcinoma cell line.
abstract: 1. The cytotoxicity and cytokinetic effects of Mitomycin C (MC) and/or photochemotherapy (PCT) in cultured human colon adenocarcinoma (WiDr) cells were investigated using colony formation to determine cell survival and DNA flow cytometry to analyze cell kinetics. 2. A low concentration of MC (0.01 micrograms/ml) caused accumulation of cells in late S and early G2 phase; higher concentrations (0.05-0.5 micrograms/ml) induced accumulation of the cells in mid and early S phase. 3. The effects of the lowest concentration of MC (0.01 micrograms/ml) were reversible upon removal of the drug, whereas a higher concentration of MC (0.1 micrograms/ml) resulted in a permanent inhibition of cell cycle progression. 4. The sensitivity of Photofrin II-loaded cells to PCT can be enhanced significantly by the addition of MC. 5. The MC-induced accumulation of the cells in S phase may be one reason for the increased cytotoxicity of PCT combined with MC. 6. The data suggest that MC may also inhibit repair of PCT-induced DNA damage.
authors.affiliation: Department of Biophysics, Institute for Cancer Research, Montebello, Oslo, Norway.
authors.forename: L W
authors.initials: LW
authors.lastname: Ma
authors.forename: H B
authors.initials: HB
authors.lastname: Steen
authors.forename: J
authors.initials: J
authors.lastname: Moan
authors.forename: K
authors.initials: K
authors.lastname: Berg
authors.forename: Q
authors.initials: Q
authors.lastname: Peng
authors.forename: H
authors.initials: H
authors.lastname: Saether
authors.forename: C
authors.initials: C
authors.lastname: Rimington
mesh_terms: D000230:Adenocarcinoma / Q000188:drug therapy* / Q000473:pathology; D002453:Cell Cycle / Q000187:drug effects; D002470:Cell Survival; D003110:Colonic Neoplasms / Q000188:drug therapy* / Q000473:pathology; D005434:Flow Cytometry; D006801:Humans; D016685:Mitomycin / Q000627:therapeutic use*; D010778:Photochemotherapy; D014407:Tumor Cells, Cultured
chemical_list: D016685:Mitomycin