title: Serotonergic modulation of the respiratory rhythm generator at birth: an in vitro study in the rat.
abstract: In order to investigate the mechanisms through which serotonin (5-HT) modulates the activity of the respiratory rhythm generator, respiratory activity was recorded from cervical ventral roots of the superfused isolated brainstem-spinal cord preparation of the newborn rat. Replacing the normal bathing medium by a medium containing 5-HT (30 microM) increased the respiratory frequency by 70% of the control value. Intact pontomedullary structures are necessary for this effect to take place, however, since the 5-HT-induced increases in respiratory frequency were no longer observed after elimination (section and electrolytic lesion) of the caudal ventro-lateral pons containing the A5 areas. Local applications of 5-HT (dual bath, microdialysis and microinjection experiments) revealed, however, that 5-HT acts at the medullary level and that its effects are not due to a diffuse action on all the neurons of the medullary respiratory centers but to a specific action focusing on structures located in the rostral ventro-lateral medulla.
authors.affiliation: Biologie des Rythmes et du Développement, URA CNRS 0205, Faculté St. Jérôme, Marseilles, France.
authors.forename: E
authors.initials: E
authors.lastname: Di Pasquale
authors.forename: D
authors.initials: D
authors.lastname: Morin
authors.forename: R
authors.initials: R
authors.lastname: Monteau
authors.forename: G
authors.initials: G
authors.lastname: Hilaire
mesh_terms: D000818:Animals; D000831:Animals, Newborn / Q000502:physiology*; D008526:Medulla Oblongata / Q000187:drug effects / Q000502:physiology*; D010507:Periodicity; D011149:Pons / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D011985:Receptors, Serotonin / Q000502:physiology; D012119:Respiration / Q000502:physiology*; D012125:Respiratory Center / Q000187:drug effects / Q000502:physiology*; D012701:Serotonin / Q000502:physiology*; D013126:Spinal Nerve Roots / Q000502:physiology; D013268:Stimulation, Chemical
chemical_list: D011985:Receptors, Serotonin; D012701:Serotonin