DNA probe for strain typing of Cryptococcus neoformans.
A 7-kb linear plasmid, harbored by a URA5 transformant, hybridized to all the chromosomes of Cryptococcus neoformans separated by contour-clamped homogeneous electric field electrophoresis. Its linear maintenance was determined to have been facilitated by the presence of telomere-like sequences at its free ends. Hybridization of this plasmid to AccI-digested genomic DNAs of 26 C. neoformans strains generated 21 unique DNA fingerprints. The DNA fingerprints of isolates within the same serotype were more similar to one another than to those from different serotypes. An acapsular clinical isolate, strain 602, widely used in immunological studies and previously thought to be in serotype D, showed DNA fingerprints typical of serotype A isolates. Isogenic strains of C. neoformans exhibited DNA fingerprints that were identical to one another. The DNA fingerprints were stable and reproducible in spite of repeated transfers in the laboratory on either complex (1% yeast extract, 2% Bacto Peptone, 2% glucose) or minimal (yeast nitrogen base) medium. The DNA fingerprints of isolates recovered from primary blood and cerebrospinal fluid cultures of patients for whom AIDS had been diagnosed showed that the original infection in each of these patients contained a homogeneous population of C. neoformans. The DNA fingerprints of isolates recovered from different tissues of infected mice and from patients undergoing different drug therapy regimens were also found to be very stable.
Varma
A
A
Clinical Mycology Section, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892.
Kwon-Chung
K J
KJ
D017088:AIDS-Related Opportunistic Infections / Q000150:complications / Q000382:microbiology; D000818:Animals; D015373:Bacterial Typing Techniques / Q000706:statistics & numerical data; D001483:Base Sequence; D015139:Blotting, Southern; D003453:Cryptococcosis / Q000150:complications / Q000382:microbiology; D003455:Cryptococcus neoformans / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D016172:DNA Fingerprinting; D015342:DNA Probes; D004269:DNA, Bacterial / Q000235:genetics; D005819:Genetic Markers; D006801:Humans; D051379:Mice; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D015183:Restriction Mapping; D012680:Sensitivity and Specificity; D012703:Serotyping; D014170:Transformation, Genetic
D015342:DNA Probes; D004269:DNA, Bacterial; D005819:Genetic Markers