Determination of monosaccharides and sugar alcohols in tissues from diabetic rats by high-performance liquid chromatography with pulsed amperometric detection.
A sensitive and simple high-performance liquid chromatographic method has been developed to determine the concentration of monosaccharides and sugar alcohols in animal tissues. Five neutral monosaccharides (D-glucose, D-galactose, D-mannose, D-fructose, and D-ribose) and three neutral sugar alcohols (myo-inositol, glycerol, and D-sorbitol) predominate in the renal cortices and sciatic nerves of rats. These monosaccharides and sugar alcohols were extracted with distilled water, purified by deproteinization with ethanol, a Sep-Pak C18 cartridge, and columns of Dowex 50W-X8 and Amberlite CG-400, then separated on Ca2+ and Pb2+ cation-exchange columns, eluted with deionized distilled water at 80 degrees C, and detected using integrated pulsed amperometry. About 10 pmol of each sugar was detectable with a signal-to-noise ratio of 10:1. D-Glucose, D-fructose, D-sorbitol, and D-mannose were higher in both the renal and sciatic tissues of diabetic rats than in those of normal animals. D-Ribose and glycerol were higher in the renal cortex of diabetic animals.
Tomiya
N
N
Mie Research Laboratory, Sanwa Kagaku Kenkyusho, Co., Ltd., Japan.
Suzuki
T
T
Awaya
J
J
Mizuno
K
K
Matsubara
A
A
Nakano
K
K
Kurono
M
M
D000818:Animals; D002241:Carbohydrates / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D004563:Electrochemistry / Q000379:methods; D007672:Kidney Cortex / Q000737:chemistry / Q000378:metabolism; D008297:Male; D009005:Monosaccharides / Q000032:analysis* / Q000302:isolation & purification / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012584:Sciatic Nerve / Q000737:chemistry / Q000378:metabolism; D013402:Sugar Alcohols / Q000032:analysis* / Q000302:isolation & purification / Q000378:metabolism
D002241:Carbohydrates; D009005:Monosaccharides; D013402:Sugar Alcohols