title: Pulsed field sequencing gel electrophoresis.
abstract: The effect of pulsed fields on sequencing gel electrophoresis is investigated, using DNA fragment markers ranging in size from 20 to 6557 bases. For high continuous electric fields (5000 V/55 cm) band inversion is observed in which fragments larger than 4000 bases migrate faster than those of 800-1000 bases. The use of one-dimensional pulsed field gel electrophoresis (ODPFGE) eliminates band inversion and extends the monotonic size-mobility relationship of the DNA markers up to about 4000 bases. The relevance of these results, obtained using a manual sequencing process with autoradiographic detection, to automated sequences is discussed.
authors.affiliation: Xerox Research Centre of Canada, Mississauga, Ontario.
authors.forename: E
authors.initials: E
authors.lastname: Brassard
authors.forename: C
authors.initials: C
authors.lastname: Turmel
authors.forename: J
authors.initials: J
authors.lastname: Noolandi
mesh_terms: D001322:Autoanalysis; D001345:Autoradiography; D004277:DNA, Single-Stranded / Q000737:chemistry*; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods*
chemical_list: D004277:DNA, Single-Stranded