M13 single-strand purification using a biotinylated probe and streptavidin coated magnetic beads.
A new method for M13 single-strand purification has been developed and tested. The procedure gives sufficient DNA for one set of thermally cycled sequencing reactions when analysed on the Applied Biosystems 373A fluorescent DNA sequencer. For this report, 250 random M13 subclones with 1-2 Kb inserts were grown and prepared using this method.
Hawkins
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MRC Laboratory of Molecular Biology, Cambridge, U.K.
D001426:Bacterial Proteins; D017104:Bacteriophage M13 / Q000235:genetics; D001483:Base Sequence; D001710:Biotin; D015342:DNA Probes; D004277:DNA, Single-Stranded / Q000302:isolation & purification*; D004279:DNA, Viral / Q000302:isolation & purification*; D008280:Magnetics; D008863:Microspheres; D008969:Molecular Sequence Data; D019809:Streptavidin; D013698:Templates, Genetic
D001426:Bacterial Proteins; D015342:DNA Probes; D004277:DNA, Single-Stranded; D004279:DNA, Viral; D001710:Biotin; D019809:Streptavidin