Inhibitory effects of endothelin-3 on vasopressin release from rat supraoptic nucleus in vitro.
Endothelin (ET) is thought to be involved in the central regulation of body water metabolism. A recent perfusion study of rat hypothalamus showed that ET has a direct stimulatory action on arginine-vasopressin (AVP) release. This study was undertaken to investigate whether ET acts directly on AVP release for supraoptic nucleus (SON) neurons, using brain slices containing only the SON. It was demonstrated that ET inhibited the AVP release dose-dependently from 10(-11) M to 10(-6) M. No difference of the effects of ET-1 and ET-3 on AVP release was observed. These results suggest that ET directly inhibits SON neurons through the ETB receptor.
Yamamoto
S
S
First Department of Internal Medicine, School of Medicine, University of Occupational and Environmental Health, Kitakyushu, Japan.
Morimoto
I
I
Yamashita
H
H
Eto
S
S
D000818:Animals; D001127:Arginine Vasopressin / Q000037:antagonists & inhibitors* / Q000378:metabolism; D016232:Endothelins / Q000494:pharmacology*; D066298:In Vitro Techniques; D008297:Male; D009994:Osmolar Concentration; D011863:Radioimmunoassay; D051381:Rats; D017208:Rats, Wistar; D013495:Supraoptic Nucleus / Q000378:metabolism*; D010566:Virulence Factors, Bordetella / Q000494:pharmacology
D016232:Endothelins; D010566:Virulence Factors, Bordetella; D001127:Arginine Vasopressin