[The effect of opioid peptides on the size of the cellular aggregates in a cell culture of rat embryonic spinal cord].
Effect of opioid peptide Leu-enkephalin (Leu-enk) and synthetic analogue of Leu-enk on fetal rat spinal cord cells in culture was studied. Activity of peptides was measured in dissociated culture on aggregation assay. The aggregated place was calculated on days 3 and 6 of culturing, because this parameter indicates the cell adhesion and neuronal and glial survival. The aggregation place was shown to increase due to peptide action: with Leu-enk and dalargin by 1.6 and 1.7, resp., compared to the control; both calculations being made on days 3 and 6 of culturing. The results of the analysis of opioid peptide activity during culturing of dissociated cells of fetal rat spinal cord demonstrate that the peptides changed the adhesion of cells and increased their survival. The results of the present and our earlier experiments suggest that the opioid peptides may be growth factors for CNS cells and may play a role in development and regeneration of the nervous tissue.
Kalenchuk
V U
VU
Kozlova
M V
MV
D000818:Animals; D002449:Cell Aggregation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured / Q000166:cytology / Q000187:drug effects; D004622:Embryo, Mammalian; D004723:Endorphins / Q000494:pharmacology*; D004743:Enkephalin, Leucine / Q000502:physiology; D016308:Enkephalin, Leucine-2-Alanine / Q000031:analogs & derivatives / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D013116:Spinal Cord / Q000166:cytology / Q000187:drug effects*; D013997:Time Factors
D004723:Endorphins; D004743:Enkephalin, Leucine; D016308:Enkephalin, Leucine-2-Alanine; C040996:enkephalin-Leu, Ala(2)-Arg(6)-
Vliianie opioidnykh peptidov na razmery kletochnykh agregatov v kul'ture kletok spinnogo mozga émbrionov krys.