title: Multiple antibiotic susceptibility associated with inactivation of the prc gene.
abstract: A Tn5 insertion which led to increased susceptibility to multiple drugs, including tetracycline, chloramphenicol, nalidixic acid, erythromycin, spectinomycin, norfloxacin, and novobiocin, was identified in Escherichia coli. Cloning and sequence studies showed that the insertion was in the previously identified prc gene at min 40.4. The prc product is known to function as a protease linked to processing of penicillin-binding protein 3 and lambda repressor and when absent to allow some leakage of periplasmic constituents. Complementation studies with the prc gene on plasmids showed complete recovery of parental levels of susceptibility to all drugs except chloramphenicol, with which only partial reversion to wild-type levels was observed.
authors.affiliation: Department of Molecular Biology, Tufts University School of Medicine, Boston, Massachusetts.
authors.forename: A
authors.initials: A
authors.lastname: Seoane
authors.forename: A
authors.initials: A
authors.lastname: Sabbaj
authors.forename: L M
authors.initials: LM
authors.lastname: McMurry
authors.forename: S B
authors.initials: SB
authors.lastname: Levy
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D001483:Base Sequence; D002352:Carrier Proteins; D003001:Cloning, Molecular; D004352:Drug Resistance, Microbial / Q000235:genetics*; D010450:Endopeptidases / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins; D005798:Genes, Bacterial / Q000235:genetics*; D005816:Genetic Complementation Test; D006602:Hexosyltransferases / Q000096:biosynthesis; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000096:biosynthesis; D002267:Muramoylpentapeptide Carboxypeptidase; D016254:Mutagenesis, Insertional; D046915:Penicillin-Binding Proteins; D046928:Peptidoglycan Glycosyltransferase; D010458:Peptidyl Transferases / Q000096:biosynthesis; D011499:Protein Processing, Post-Translational
chemical_list: D001426:Bacterial Proteins; D002352:Carrier Proteins; D029968:Escherichia coli Proteins; C478704:FtsI protein, E coli; D009097:Multienzyme Complexes; D046915:Penicillin-Binding Proteins; D010458:Peptidyl Transferases; D006602:Hexosyltransferases; D046928:Peptidoglycan Glycosyltransferase; D010450:Endopeptidases; D002267:Muramoylpentapeptide Carboxypeptidase