Structure and chromosomal localization of human arachidonate 12-lipoxygenase gene.
Arachidonate 12-lipoxygenase introduces a molecular oxygen into the C-12 position of arachidonic acid to produce 12(S)-hydroperoxy-5,8,10,14-eicosatetraenoic acid. With the aid of cDNA probes for the enzyme, we isolated overlapping lambda clones encompassing the human 12-lipoxygenase gene and flanking regions from a human genomic library. The gene consists of 14 exons with 13 introns and spans approximately 15 kilobases of DNA. All the exon-intron junctions conform to the GT/AG rule. Neither a typical TATA box nor a CAAT box was found in approximately 1-kb sequence of 5'-upstream region of the translation initiation site. However, this region contains several regulatory elements including four GC boxes, two CACCC boxes, three AP-2 binding sequences, and a glucocorticoid-responsive element. The major transcription initiation site was determined by primer-extension analysis as an adenosine residue at 306 bases upstream from the translation initiation codon. The chromosomal localization of the human 12-lipoxygenase gene was examined by fluorescence in situ hybridization, and the gene was assigned to the sub-band p13.1 of chromosome 17.
Yoshimoto
T
T
Department of Biochemistry, Tokushima University, School of Medicine, Japan.
Arakawa
T
T
Hada
T
T
Yamamoto
S
S
Takahashi
E
E
D001092:Arachidonate 12-Lipoxygenase / Q000235:genetics*; D001483:Base Sequence; D001792:Blood Platelets / Q000201:enzymology; D015139:Blotting, Southern; D002871:Chromosome Banding; D002874:Chromosome Mapping; D002886:Chromosomes, Human, Pair 17; D004247:DNA / Q000097:blood / Q000235:genetics / Q000302:isolation & purification; D005091:Exons; D015698:Genomic Library; D006801:Humans; D017403:In Situ Hybridization; D007438:Introns; D007621:Karyotyping; D007962:Leukocytes / Q000201:enzymology; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D014176:Protein Biosynthesis; D012045:Regulatory Sequences, Nucleic Acid; D015183:Restriction Mapping; D014407:Tumor Cells, Cultured
D009838:Oligodeoxyribonucleotides; D004247:DNA; D001092:Arachidonate 12-Lipoxygenase