[The detection and quantitative determination of antigens to the human immunodeficiency virus types 1 and 2].
Three modifications of ELISA test system for HIV antigen detection are described. They are based on IgG from HIV-1 and HIV-2-infected human sera and monoclonal antibodies against HIV-1 p24 used as immunosorbents. The peroxidase/anti-HIV-IgG conjugate was used in all the test systems. A possibility of quantitative detection of viral antigen in native culture fluids, lysates, and purified virus preparations was demonstrated. The test system for HIV-1 antigen detection cannot be used for HIV-2 antigen detection and vice versa. The diagnostic value of HIV-1 p24 antigen detection consists in the possibility of earlier AIDS identification and monitoring of the disease at various stages. The sensitivity of "p24" assay is 0.5 ng/ml.
Fediuk
N V
NV
Konovalov
E E
EE
Loktev
V B
VB
Uryvaev
L V
LV
Kuliandin
S A
SA
Pokrovskiĭ
A G
AG
D002460:Cell Line; D002478:Cells, Cultured / Q000382:microbiology; D015488:HIV Antigens / Q000097:blood*; D016655:HIV Core Protein p24 / Q000097:blood; D006679:HIV Seropositivity / Q000276:immunology; D015497:HIV-1 / Q000276:immunology*; D015498:HIV-2 / Q000276:immunology*; D006801:Humans; D015151:Immunoblotting; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000097:blood; D008214:Lymphocytes / Q000382:microbiology; D014776:Virus Cultivation
D015488:HIV Antigens; D016655:HIV Core Protein p24; D007074:Immunoglobulin G
Vyiavlenie i kolichestvennoe opredelenie antigenov virusa immunodefitsita cheloveka tipov 1 i 2.