title: [The use of immunoenzyme analysis for determining ABH antigens in traces of saliva and sperm].
abstract: The authors suggest a simple sensitive technique for enzyme immunoassay (EIA) of ABH antigens in saliva and semen. A two-staged dot blot solid-phase EIA on nitrocellulose membranes was employed with anti-ABH monoclonal antibodies obtained in immunization of mice with human red cells. 4-chloro-1-naphthol substrate solution was used to visualize the peroxidase label. The results of analysis of salivary and spermatic samples obtained from donors of various groups evidence that this EIA variant may be useful in forensic medicine.
authors.forename: E I
authors.initials: EI
authors.lastname: Deriugina
authors.forename: Iu I
authors.initials: IuI
authors.lastname: Savel'ev
authors.forename: A E
authors.initials: AE
authors.lastname: Nosyrev
authors.forename: M I
authors.initials: MI
authors.lastname: Lapenkov
authors.forename: T L
authors.initials: TL
authors.lastname: Nikolaeva
mesh_terms: D000017:ABO Blood-Group System / Q000276:immunology*; D000911:Antibodies, Monoclonal; D000941:Antigens / Q000032:analysis*; D002021:Buffers; D006801:Humans; D015151:Immunoblotting / Q000379:methods; D007124:Immunoenzyme Techniques; D007202:Indicators and Reagents; D007983:Lewis Blood Group Antigens / Q000276:immunology; D008297:Male; D012463:Saliva / Q000737:chemistry* / Q000276:immunology; D013094:Spermatozoa / Q000737:chemistry* / Q000276:immunology; D013997:Time Factors
chemical_list: D000017:ABO Blood-Group System; D000911:Antibodies, Monoclonal; D000941:Antigens; D002021:Buffers; D007202:Indicators and Reagents; D007983:Lewis Blood Group Antigens
vernacular_title: Primenenie immunofermentnogo analiza dlia opredeleniia ABH-antigenov v sledakh sliuny i spermy.