title: Low calcium/high magnesium medium increases activities of hypothalamic arcuate and suprachiasmatic neurons in brain tissue slices.
abstract: Extracellular single-unit recording was conducted in hypothalamic arcuate (ARC) and suprachiasmatic nucleus (SCN) in rat brain slices. The perifusion medium was switched from normal artificial cerebrospinal fluid (ACSF) to ACSF containing low Ca2+ and high Mg2+ to test the effect of extracellular calcium on the spontaneous activities of these neurons and their responses to some test agents. It was found that almost all of the ARC and SCN units tested increased their firing rates in low Ca2+, high Mg2+ ACSF, and exhibited more significant responses to excitatory test agents. The effects of low Ca2+, high Mg2+ ACSF were repeatable and reversible. The results suggest that extracellular Ca2+ is essential in maintaining the membrane stability of most hypothalamic neurons and most agents tested are acting postsynaptically on the unit recorded.
authors.affiliation: Institute of Physiology, National Yang-Ming Medical College, Taipei, Taiwan, ROC.
authors.forename: J T
authors.initials: JT
authors.lastname: Pan
authors.forename: C S
authors.initials: CS
authors.lastname: Li
authors.forename: K C
authors.initials: KC
authors.lastname: Tang
authors.forename: J Y
authors.initials: JY
authors.lastname: Lin
mesh_terms: D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D001111:Arcuate Nucleus of Hypothalamus / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D002118:Calcium / Q000502:physiology*; D005260:Female; D066298:In Vitro Techniques; D008274:Magnesium / Q000494:pharmacology*; D009474:Neurons / Q000187:drug effects / Q000378:metabolism; D010052:Ovariectomy; D051381:Rats; D017207:Rats, Sprague-Dawley; D013493:Suprachiasmatic Nucleus / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*
chemical_list: D008274:Magnesium; D002118:Calcium