Endrin-induced urinary excretion of formaldehyde, acetaldehyde, malondialdehyde and acetone in rats.
Previous studies have shown that endrin induces an oxidative stress in rats as demonstrated by an increase in hepatic lipid peroxidation, a decrease in glutathione content and a decrease in the activity in selenium-dependent glutathione peroxidase. We have therefore examined the effects of orally administering 1.5, 3.0, 4.5 and 6.0 mg endrin/kg on the urinary excretion of the lipid metabolites formaldehyde, malondialdehyde, acetaldehyde and acetone. The simultaneous determination of these four lipid metabolites may be a useful biomarker for assessing exposure to xenobiotics which induce an oxidative stress and enhanced lipid peroxidation. Urine samples were collected up to 72 h post-treatment. The identities of the lipid metabolites were confirmed by gas chromatography-mass spectroscopy, while the 2,4-dinitrophenylhydrazine derivatives of these metabolic products were quantitated by high pressure liquid chromatography. Maximum increases in the excretion of the four lipid metabolites occurred at approx. 24 h post-treatment at all doses with no significant increases in excretion occurring thereafter. The maximum increases in excretion of malondialdehyde, formaldehyde, acetaldehyde and acetone were approx. 160%, 93%, 121% and 162%, respectively, relative to control values. Seventy-two hours after endrin administration, the liver weight/body weight and spleen weight/body weight ratios significantly increased while the thymus weight/body weight ratio markedly decreased. The results demonstrate that endrin induces dose- and time-dependent alterations in lipid metabolism with the enhanced excretion of specific metabolic products in the urine.
Bagchi
D
D
School of Pharmacy and Allied Health Professions, Creighton University, Omaha, NE 68178.
Bagchi
M
M
Hassoun
E
E
Stohs
S J
SJ
D000079:Acetaldehyde / Q000652:urine; D000096:Acetone / Q000652:urine*; D000447:Aldehydes / Q000652:urine*; D000818:Animals; D015415:Biomarkers / Q000652:urine; D001835:Body Weight / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004732:Endrin / Q000633:toxicity*; D005260:Female; D005557:Formaldehyde / Q000652:urine; D050356:Lipid Metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000033:anatomy & histology / Q000187:drug effects; D008315:Malondialdehyde / Q000652:urine; D009929:Organ Size / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D013154:Spleen / Q000033:anatomy & histology / Q000187:drug effects; D013950:Thymus Gland / Q000033:anatomy & histology / Q000187:drug effects; D013997:Time Factors
D000447:Aldehydes; D015415:Biomarkers; D000096:Acetone; D005557:Formaldehyde; D008315:Malondialdehyde; D000079:Acetaldehyde; D004732:Endrin