[Site-directed chemical restriction of a single-stranded DNA fragment by an alkylating derivative of the tetranucleotide d(pApGpCpA) in the presence of tetranucleotide effectors].
Alkylation of a single-stranded DNA 302-mer by a 5'-O-phosphoryl-[4-(N-2-chloroethyl-N-methylamino)benzyl]amide derivative of the tetradeoxyribonucleotide d(pApGpCpA) in the presence of 3',5'-di-N-(2-hydroxyethyl) phenazinium derivatives of tetranucleotides as effectors led to specific chemical cleavage of the target at the guanosine residues of the sites ... pTpGppT. The reagent can be selectively addressed to one of three alkylation sites with the aid of a pair of tetranucleotide effectors flanking the chemically reactive tetranucleotide in the complex with the target DNA. The yield of the cleavage depends on the concentration of both the reagent and effectors, and can be enhanced, if a chain of two or more effectors from each side of the reagent is used. In this case, 3',5'-di-Phn-tetranucleotide effectors are to immediately flank the reagent.
Zarytova
V F
VF
Kutiavin
I V
IV
Mamaev
S V
SV
Podyminogin
M A
MA
D000478:Alkylation; D001345:Autoradiography; D001483:Base Sequence; D004277:DNA, Single-Stranded / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D006868:Hydrolysis; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000737:chemistry*
D004277:DNA, Single-Stranded; D009841:Oligonucleotides
Saĭtnapravlennaia khimicheskaia restriktsiia odnotsepochechnogo fragmenta DNK alkiliruiushchim proizvodnym tetranukleotida d(pApGpCpA) v prisutstvii tetranukleotidnykh éffektorov.