title: The restricted nature of HIV-1 tropism for cultured neural cells.
abstract: Infection of the central nervous system by HIV-1, the agent of AIDS, is characterized by the presence of infected and giant microglial cells as well as astrocytosis, demyelination, and neuronal loss. To determine whether cells of neuroectoderm origin can be infected by HIV-1, we have inoculated primary cultures derived from adult human brain with a lymphotropic virus (LAV) or a neurotropic virus (Jr-FL) isolated from a patient with AIDS dementia. While Jr-FL invariably causes productive infection of cultured brain microglia, neither astrocytes nor oligodendrocytes became productively infected by these viral strains. Moreover, the cultured oligodendrocytes develop a normal network of processes and express differentiation antigens in the presence of an ongoing lytic infection of microglial cells. No HIV-1 proviral DNA was detected in primary astrocyte cultures devoid of microglial after inoculation of either HIV-1 strain. Similarly, the neuronal cell line HCN-1 in its differentiated state did not allow the virus to go through cycles of reverse transcription and replication. LAV, however, was able to replicate in undifferentiated HCN-1 cells. Thus, tropism of HIV-1 appears tightly restricted to only one type of differentiated cell in the CNS, the microglia.
authors.affiliation: Laboratory of Viral and Molecular Pathogenesis, National Institute of Neurological Disorders and Stroke, Bethesda, Maryland 20892.
authors.forename: N
authors.initials: N
authors.lastname: Sharpless
authors.forename: D
authors.initials: D
authors.lastname: Gilbert
authors.forename: B
authors.initials: B
authors.lastname: Vandercam
authors.forename: J M
authors.initials: JM
authors.lastname: Zhou
authors.forename: E
authors.initials: E
authors.lastname: Verdin
authors.forename: G
authors.initials: G
authors.lastname: Ronnett
authors.forename: E
authors.initials: E
authors.lastname: Friedman
authors.forename: M
authors.initials: M
authors.lastname: Dubois-Dalcq
mesh_terms: D015526:AIDS Dementia Complex / Q000382:microbiology*; D001253:Astrocytes / Q000382:microbiology*; D001921:Brain / Q000166:cytology / Q000382:microbiology*; D002454:Cell Differentiation; D002478:Cells, Cultured; D004279:DNA, Viral / Q000032:analysis; D016655:HIV Core Protein p24 / Q000378:metabolism; D015497:HIV-1 / Q000254:growth & development* / Q000302:isolation & purification; D006801:Humans; D007150:Immunohistochemistry; D007930:Leucine / Q000031:analogs & derivatives / Q000494:pharmacology; D009836:Oligodendroglia / Q000187:drug effects / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D011533:Proviruses / Q000737:chemistry; D014018:Tissue Distribution; D014158:Transcription, Genetic; D014779:Virus Replication
chemical_list: D004279:DNA, Viral; D016655:HIV Core Protein p24; C030365:leucine methyl ester; D007930:Leucine