Interferon-gamma inhibits proliferation, but not commitment, of murine granulocyte-macrophage progenitors.
We investigated the effects of interferon gamma (IFN-gamma) on the growth of murine hematopoietic progenitors. IFN-gamma inhibited granulocyte colony-stimulating factor (G-CSF)- and interleukin-3 (IL-3)-dependent colony growth by granulocyte-macrophage (GM) progenitors derived from the bone marrow cells of normal mice. However, the number of IL-3-dependent GM colonies formed by the bone marrow cells of 5-fluorouracil (5-FU)-treated mice was not influenced by the addition of IFN-gamma. Replating experiments suggested that IFN-gamma suppressed GM colony growth directly and that it exerted an inhibitory effect on the proliferation, but not on the commitment, of GM progenitors. In contrast, IFN-gamma failed to suppress colony growth by mast cell progenitors. Erythroid and megakaryocytic progenitors exhibited different responses to IFN-gamma depending on mouse strains. These results suggest that potent negative regulators are not always inhibitors of hematopoietic progenitors.
Koike
K
K
Department of Pediatrics, Shinshu University School of Medicine, Matsumoto, Japan.
Ma
F
F
Shiohara
M
M
Kubo
T
T
Takagi
M
M
Amano
Y
Y
Nakahata
T
T
Komiyama
A
A
D000818:Animals; D001854:Bone Marrow Cells; D002455:Cell Division / Q000187:drug effects; D005260:Female; D005472:Fluorouracil / Q000494:pharmacology; D006098:Granulocytes / Q000502:physiology*; D007371:Interferon-gamma / Q000494:pharmacology*; D008264:Macrophages / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D012016:Reference Values; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000502:physiology
D007371:Interferon-gamma; D005472:Fluorouracil