Analysis of the autonomous replication behavior in human cells of the dihydrofolate reductase putative chromosomal origin of replication.
Chinese hamster genomic DNA sequences from the region downstream of the dihydrofolate reductase (DHFR) gene reported to contain a chromosomal origin of bidirectional DNA replication (OBR-1) were tested for their ability to support autonomous DNA replication in human cells. A 13.3 kilobase fragment containing OBR-1 and surrounding sequences supported replication in short-term and long-term replication assays, while a 4.5 kb fragment containing OBR-1 did not support substantial replication in either assay. These results are consistent with our previous observations that large fragments of human DNA support replication, while smaller fragments are less efficient. The replication activities of plasmids containing OBR-1 were no greater than those of randomly chosen human fragments of similar size. Furthermore, two-dimensional gel analysis of plasmids containing OBR-1 indicated that initiation does not preferentially occur within the OBR-1 region. These results suggest that in the context of autonomous replication, the DHFR sequences tested do not contain genetic information specifying site-specific replication initiation. Possible implications of these results for chromosomal replication are discussed.
Caddle
M S
MS
Department of Genetics, Stanford University School of Medicine, CA 94305.
Calos
M P
MP
D000818:Animals; D002460:Cell Line; D002875:Chromosomes / Q000378:metabolism; D003001:Cloning, Molecular; D006224:Cricetinae; D004261:DNA Replication; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D010957:Plasmids; D012093:Replicon; D015183:Restriction Mapping; D013762:Tetrahydrofolate Dehydrogenase / Q000235:genetics* / Q000378:metabolism
D013762:Tetrahydrofolate Dehydrogenase