title: A postsynaptic GABA transporter in rat spinal motor neurones.
abstract: The plasma membrane uptake system for gamma-amino butyric acid (GABA) is conventionally assumed to be presynaptic, so that after release GABA could be taken up and incorporated into synaptic vesicles for re-use. Using in situ hybridization histochemistry we find that rat spinal motor neurones express GAT-1, a transporter protein for GABA, but that they do not express glutamic acid decarboxylase, the synthetic enzyme for GABA. We conclude that the uptake system is located postsynaptically in these cells. Our observation may explain previous reports where GABA has been detected immunocytochemically in motor neurones.
authors.affiliation: Department of Anatomy and Cell Biology, St Mary's Hospital Medical School, Imperial College, London, UK.
authors.forename: H
authors.initials: H
authors.lastname: Snow
authors.forename: M B
authors.initials: MB
authors.lastname: Lowrie
authors.forename: J P
authors.initials: JP
authors.lastname: Bennett
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000032:analysis*; D050485:GABA Plasma Membrane Transport Proteins; D015870:Gene Expression; D005968:Glutamate Decarboxylase / Q000032:analysis; D017403:In Situ Hybridization; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D009046:Motor Neurons / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000032:analysis*; D027361:Organic Anion Transporters; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D013116:Spinal Cord / Q000166:cytology*; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
chemical_list: D002352:Carrier Proteins; D050485:GABA Plasma Membrane Transport Proteins; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D009419:Nerve Tissue Proteins; D027361:Organic Anion Transporters; D012333:RNA, Messenger; C493415:Slc6a1 protein, rat; D005680:gamma-Aminobutyric Acid; D005968:Glutamate Decarboxylase