Effect of dequalinium on K1735-M2 melanoma cell growth, directional migration and invasion in vitro.
Cationic lipophilic compounds have an antiproliferative effect on certain tumour systems in vitro and in vivo. We have investigated whether the cationic lipophilic compound dequalinium affects not only proliferation but also motility and invasion of the highly metastatic and highly invasive melanoma cell line K1735-M2. Proliferation was assessed in monolayer cultures and in multicellular spheroids, motility was estimated in the assay of directional migration, and invasiveness was tested through confrontation cultures of tumour multicellular spheroids with embryonic chick heart tissue evaluated by computerized image analysis. 2 mumol/l dequalinium impaired melanoma cell proliferation, reduced directional migration and significantly blocked invasion in vitro. On the ultrastructural level, dequalinium caused obvious changes in mitochondria of both melanoma and embryonic chick heart cells. The mechanisms of the antiproliferative, antimigrating and antiinvasive effects remain to be determined. Inhibition of protein kinase C, calmodulin antagonism, DNA intercalation and/or direct effects on mitochondrial functions may be considered.
Helige
C
C
Department of Dermatology and Venerology, University of Graz, Austria.
Smolle
J
J
Zellnig
G
G
Fink-Puches
R
R
Kerl
H
H
Tritthart
H A
HA
D000818:Animals; D003868:Dequalinium / Q000627:therapeutic use*; D008545:Melanoma / Q000188:drug therapy* / Q000648:ultrastructure; D051379:Mice; D008854:Microscopy, Electron; D008928:Mitochondria / Q000648:ultrastructure; D008938:Mitosis / Q000187:drug effects; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D014407:Tumor Cells, Cultured / Q000187:drug effects
D003868:Dequalinium