Measurement of blood-brain barrier permeability in a tumor model using magnetic resonance imaging with gadolinium-DTPA.
Sequential MR imaging with gadolinium-diethylenetriaminepentaacetic acid (Gd-DTPA) and sequential measurements of plasma Gd-DTPA concentration by inductively coupled plasma atomic emission spectroscopy (ICP-AES) were used to estimate the blood-to-tissue transport coefficient (Ki) in the 36B-10 rat glioma model. For these measurements, tissue Gd-DTPA concentration was estimated from tumor enhancement by correlation with calibration measurements obtained by ICP-AES analysis of tumor tissue. The 14 animals for which Ki was calculated can be grouped into those imaged at 11 days following tumor implantation, at 13-18 days, and at 20 days. The mean (+SEM) Ki values for these groups were 1.1 + 0.24, 9.2 + 0.8, and 13.4 + 1.7 ml/kg-min, respectively. These results correspond well with published data obtained by quantitative autoradiography. It is concluded that frequent sequential imaging and a graphical approach to Ki calculation are promising methods for determining the blood-to-tissue transport coefficient noninvasively by contrast-enhanced MRI.
Kenney
J
J
Department of Radiology, University of Washington School of Medicine, Seattle.
Schmiedl
U
U
Maravilla
K
K
Starr
F
F
Graham
M
M
Spence
A
A
Nelson
J
J
D000818:Animals; D001254:Astrocytoma / Q000139:chemically induced / Q000503:physiopathology*; D001812:Blood-Brain Barrier / Q000502:physiology*; D001932:Brain Neoplasms / Q000139:chemically induced / Q000503:physiopathology*; D003287:Contrast Media; D005260:Female; D005682:Gadolinium; D019786:Gadolinium DTPA; D008279:Magnetic Resonance Imaging; D008297:Male; D009368:Neoplasm Transplantation; D009942:Organometallic Compounds; D004369:Pentetic Acid; D010539:Permeability; D051381:Rats; D011916:Rats, Inbred F344
D003287:Contrast Media; D009942:Organometallic Compounds; D004369:Pentetic Acid; D005682:Gadolinium; D019786:Gadolinium DTPA