[Determination of mannitol and lactulose in urine by capillary gas chromatography].
Gas capillary chromatography (GCC) determination of mannitol and lactulose in urine after oral intake is a method for assessing the intestinal permeability in various bowel diseases. The method proposed, using gas capillary chromatography with flame ionization detection after silylation of urine residue, gives good results: coefficients of variation varied from 6 to 8.7% for mannitol and 7.5 to 13.7% for lactulose. Detection limit was 5 mg/l for both compounds.
Debord
P
P
Laboratoire de pharmacie et toxicologie, hôpital Necker-Enfants Malades, Paris, France.
Postaire
M
M
Pham-Huy
C
C
Hamon
M
M
D005410:Flame Ionization / Q000379:methods*; D006801:Humans; D007792:Lactulose / Q000652:urine*; D008353:Mannitol / Q000652:urine*
D008353:Mannitol; D007792:Lactulose
Dosage par chromatographie gazeuse capillaire du mannitol et du lactulose dans les urines.