Immunocytochemical detection of oncoproteins in animal and human tumor lines with acquired or inherent multidrug resistance.
In this presentation, we analyze the relationship between protein expression of the protooncogenes c-fos, c-erb B1, c-K-ras, and c-myc and acquired or inherent multidrug resistance (MDR) in a panel of animal (CHO, S 180, L 1210) and human (KTCTL 44, KTCTL 18, CX 1, CXF 94) tumor lines by means of immunocytochemistry. Increased expression of c-fos and c-erb B1 proteins is a constant feature of all resistant cell lines except L 1210 cells, which did not reveal any alteration in oncoprotein expression. No apparent relationship between c-K-ras and c-myc proteins and MDR was found. The immunocytochemical results were corroborated by radioimmunoassays (RIAs). The data indicate that c-fos and c-erb B1 might play a role in the development of MDR.
Efferth
T
T
German Cancer Research Center, Heidelberg.
Volm
M
M
D000230:Adenocarcinoma / Q000235:genetics; D000818:Animals; D016466:CHO Cells / Q000502:physiology; D002292:Carcinoma, Renal Cell / Q000235:genetics; D002455:Cell Division / Q000502:physiology; D003110:Colonic Neoplasms / Q000235:genetics; D006224:Cricetinae; D004351:Drug Resistance / Q000235:genetics*; D005260:Female; D015870:Gene Expression / Q000235:genetics; D006801:Humans; D007150:Immunohistochemistry; D007680:Kidney Neoplasms / Q000235:genetics; D007939:Leukemia L1210 / Q000235:genetics; D051379:Mice; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000235:genetics*; D015513:Oncogene Proteins / Q000032:analysis* / Q000235:genetics; D009857:Oncogenes / Q000235:genetics / Q000502:physiology; D012510:Sarcoma 180 / Q000235:genetics; D014407:Tumor Cells, Cultured
D015513:Oncogene Proteins