title: [Microradiography in the study of trabecular parameters].
abstract: The intermethod variation in measurement of trabecular bone volume (VTO) and the indirect estimation of its microstructure according to Parfitt MTPT (microns), MTPD (/mm) and MTPS (microns) were evaluated in seven undecalcified bone biopsies by analyzing the microradiograph of a 100-microns-thick section with an automatic method (IBAS II Zeiss, Munich) in addition to reference methods (manual and semiautomatic) described in the literature and performed on 7-microns-thick stained sections. Three consecutive 7-microns-stained sections and one 100-mu-thick microradiographed section were taken in each specimen. The 100 microns-thick section was also superficially stained. Trabecular bone volume was measured with both a manual integrating eyepiece and an automatic method. The automatic method on the microradiograph underestimated the VTO by 24.42%. There was a correlation (r = 0.75; p < 0.02) between the manual and computerized methods. Mean trabecular plate thickness (microns), mean trabecular plate density (/mm) and mean trabecular separation (microns) were measured with both semiautomatic and automatic methods. The automatic method on the microradiograph underestimated the MTPT (microns) by 18.98% and the MTPD (/mm) by 14.14% and overestimated the MTPS (microns) by 23.17%. For the MTPT (microns) there was a correlation (r = 0.88; p < 0.02), between both methods, and the correlation was good for MTPD (/mm) (r = 0.97; p < 0.001), and MTPS (r = 0.86; p < 0.002).
authors.affiliation: Institut de Recherche sur les Maladies du Squelette, Institut Calot, Berck/s/Mer, France.
authors.forename: B
authors.initials: B
authors.lastname: Flautre
authors.forename: P
authors.initials: P
authors.lastname: Hardouin
mesh_terms: D001699:Biometry / Q000379:methods; D001706:Biopsy; D001842:Bone and Bones / Q000166:cytology / Q000000981:diagnostic imaging*; D006652:Histological Techniques; D006801:Humans; D008852:Microradiography / Q000379:methods*
vernacular_title: La microradiographie dans l'étude des paramètres trabéculaires.