title: Augmentation of antineoplastic effects by the combination of recombinant human tumor necrosis factor and mitoxantrone on primary culture of human ovarian cancer cells.
abstract: Recombinant human tumor Necrosis Factor (rHuTNF) produced dose-dependent cytotoxicity against human ovarian cancer cells, OSC and OMC, obtained from fresh ascites. A combination of rHuTNF and the topoisomerase II inhibitor, Mitoxantrone, produced dose-dependent synergistic cytotoxicity on OSC and OMC cells. When OMC cells were incubated simultaneously for one hour with rHuTNF and Mitoxantrone, increased numbers of DNA single-strands breaks were produced. rHuTNF alone did not induce DNA single-strands breaks. These data are consistent with a role for topoisomerase-linked DNA lesions in the rHuTNF mediated potentiation of killing cells by Mitoxantrone.
authors.affiliation: Department of Chemical Carcinogenesis, Instituto Nazionale per la Ricerca sul Cancro, Genova, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Cimoli
authors.forename: M
authors.initials: M
authors.lastname: Valenti
authors.forename: M
authors.initials: M
authors.lastname: Venturini
authors.forename: P
authors.initials: P
authors.lastname: Conte
authors.forename: P
authors.initials: P
authors.lastname: Russo
mesh_terms: D002470:Cell Survival / Q000187:drug effects; D004249:DNA Damage; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D005260:Female; D006801:Humans; D008942:Mitoxantrone / Q000494:pharmacology*; D010051:Ovarian Neoplasms; D011994:Recombinant Proteins / Q000494:pharmacology; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*; D014410:Tumor Stem Cell Assay
chemical_list: D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; D008942:Mitoxantrone