title: Uniparental cytogamy: a novel method for bringing micronuclear mutations of Tetrahymena into homozygous macronuclear expression with precocious sexual maturity.
abstract: A new method of inducing self-fertilization, uniparental cytogamy, yields homozygous germinal and somatic genotypes in the ciliate Tetrahymena thermophila. Progeny are highly fertile and show a marked tendency for precocious sexual maturity. This method is highly effective in protocols designed to generate and express nonlethal dominant or recessive mutations.
authors.affiliation: Department of Genetics and Development, Cornell University, Ithaca, New York 14853.
authors.forename: E S
authors.initials: ES
authors.lastname: Cole
authors.forename: P J
authors.initials: PJ
authors.lastname: Bruns
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000648:ultrastructure; D003470:Culture Media; D004351:Drug Resistance; D015870:Gene Expression; D006720:Homozygote; D016296:Mutagenesis; D009154:Mutation; D009994:Osmolar Concentration; D011003:Ploidies; D016808:Tetrahymena thermophila / Q000235:genetics* / Q000254:growth & development; D013997:Time Factors
chemical_list: D003470:Culture Media