title: The murine luteinizing hormone and follicle-stimulating hormone receptor genes: transcription initiation sites, putative promoter sequences and promoter activity.
abstract: The putative promoter regions of the murine follicle-stimulating hormone (FSH) and luteinizing hormone (LH) receptor genes were isolated and used to map transcription initiation sites for both genes. For the FSH receptor gene, a major transcription initiation site was found 534 nucleotides upstream, and for the LH receptor gene 310 nucleotides upstream of the corresponding translation initiation codons. In addition, several alternative minor transcription initiation sites were observed for both genes. The nucleotide sequences of the promoter regions revealed no canonical promoter elements, such as TATA and CCAAT consensus sites 5' of the main transcriptional start sites. The isolated promoter segments for both receptor genes showed low functional activity as verified in transient expression studies in immature rat granulosa cells using the luciferase coding region as the reporter for promoter activity. Both promoter elements seem to be still under tissue specific control, since neither LH receptor nor FSH receptor promoter activity was detectable in another cell line (CHO) investigated.
authors.affiliation: Laboratory of Molecular Neuroendocrinology, University of Heidelberg, Germany.
authors.forename: I T
authors.initials: IT
authors.lastname: Huhtaniemi
authors.forename: V
authors.initials: V
authors.lastname: Eskola
authors.forename: P
authors.initials: P
authors.lastname: Pakarinen
authors.forename: T
authors.initials: T
authors.lastname: Matikainen
authors.forename: R
authors.initials: R
authors.lastname: Sprengel
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005796:Genes; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D011962:Receptors, FSH / Q000096:biosynthesis / Q000235:genetics*; D011974:Receptors, LH / Q000096:biosynthesis / Q000235:genetics*; D012045:Regulatory Sequences, Nucleic Acid; D014158:Transcription, Genetic
chemical_list: D011962:Receptors, FSH; D011974:Receptors, LH