CD8 T cells are not required for islet destruction induced by a CD4+ islet-specific T-cell clone.
A panel of CD4+ T-cell clones has been isolated from the spleen and lymph nodes of diabetic NOD mice. These clones have been shown to be islet-specific both in vivo and in vitro. One of the clones, BDC-6.9, initiates extensive damage to islet tissue when placed adjacent to an NOD islet graft that has been used to reverse diabetes in (CBA x NOD)F1 recipients or when injected intraperitoneally into such animals. In this study, we show that BDC-6.9 T cells can initiate islet destruction in the absence of detectable CD8 T cells either in the periphery or in the lesion that develops after the transfer of the cloned islet-reactive T cells.
Bradley
B J
BJ
Barbara Davis Center for Childhood Diabetes, University of Colorado Health Sciences Center, Denver 80262.
Haskins
K
K
La Rosa
F G
FG
Lafferty
K J
KJ
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D001786:Blood Glucose / Q000378:metabolism; D015704:CD4 Antigens / Q000276:immunology*; D016827:CD8 Antigens / Q000276:immunology*; D002478:Cells, Cultured; D003433:Crosses, Genetic; D003922:Diabetes Mellitus, Type 1 / Q000097:blood / Q000276:immunology* / Q000601:surgery; D005260:Female; D005434:Flow Cytometry; D007515:Islets of Langerhans / Q000276:immunology*; D016381:Islets of Langerhans Transplantation / Q000276:immunology* / Q000473:pathology; D016030:Kidney Transplantation / Q000276:immunology* / Q000473:pathology; D008198:Lymph Nodes / Q000276:immunology; D008212:Lymphocyte Depletion; D008297:Male; D051379:Mice; D016688:Mice, Inbred NOD; D008815:Mice, Inbred Strains; D013154:Spleen / Q000276:immunology; D016176:T-Lymphocyte Subsets / Q000276:immunology*
D000911:Antibodies, Monoclonal; D001786:Blood Glucose; D015704:CD4 Antigens; D016827:CD8 Antigens