[Cloning the asd and lysC genes from Cornyebacterium glutamicum].
Plasmids carrying an asd gene from a mutant. S-(2-aminoaethyl)-L-cysteine resistant strain of Corynebacterium glutamicum were selected from a clonoteque constructed on a plasmid cloning vector pSL5 by complementation of asd mutation in Escherichia coli. Evidence has been obtained that the cloned chromosomal DNA fragment contains also a complete sequence for feed-back-resistant aspartokinase lysC gene.
Peredel'chuk
M Iu
MIu
Bukanov
N O
NO
Smirnov
Iu V
IuV
Rostova
Iu V
IuV
Fedorova
N D
ND
Okorokov
A L
AL
Gusiatiner
M M
MM
Iankovskiĭ
N K
NK
D002876:Chromosomes, Bacterial; D003001:Cloning, Molecular; D003352:Corynebacterium / Q000235:genetics*; D003545:Cysteine / Q000031:analogs & derivatives / Q000494:pharmacology; D004352:Drug Resistance, Microbial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D009154:Mutation; D010957:Plasmids; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology
D011500:Protein Synthesis Inhibitors; C000204:S-2-aminoethyl cysteine; D003545:Cysteine
Klonirovanie genov asd i LysC Corynebacterium glutamicum.