Two reactions are simultaneously catalyzed by a single enzyme: the arginine-dependent simultaneous formation of two products, ethylene and succinate, from 2-oxoglutarate by an enzyme from Pseudomonas syringae.
A single enzyme isolated from Pseudomonas syringae pv. phaseolicola PK2 simultaneously catalyzed two reactions, namely, the formation of ethylene and succinate from 2-oxoglutarate, at a molar ratio of 2:1. In the main reaction, 2-oxoglutarate was dioxygenated to produce one molecule of ethylene and three molecules of carbon dioxide. In the sub-reaction, both 2-oxoglutarate and L-arginine were mono-oxygenated to yield succinate plus carbon dioxide and L-hydroxyarginine, respectively, the latter being further transformed to guanidine and L-delta 1-pyrroline-5-carboxylate. We propose a dual-circuit mechanism for the entire reaction, in which the binding of L-arginine and 2-oxoglutarate in a Schiff-base structure generates a common intermediate for two reactions.
Fukuda
H
H
Department of Applied Microbial Technology, Kumamoto Institute of Technology, Japan.
Ogawa
T
T
Tazaki
M
M
Nagahama
K
K
Fujii
T
T
Tanase
S
S
Morino
Y
Y
D001120:Arginine / Q000378:metabolism*; D005030:Ethylenes / Q000378:metabolism*; D007656:Ketoglutaric Acids / Q000378:metabolism*; D007700:Kinetics; D008190:Lyases / Q000302:isolation & purification / Q000378:metabolism*; D011549:Pseudomonas / Q000201:enzymology*; D013386:Succinates / Q000378:metabolism*; D019802:Succinic Acid
D005030:Ethylenes; D007656:Ketoglutaric Acids; D013386:Succinates; C036216:ethylene; D001120:Arginine; D019802:Succinic Acid; D008190:Lyases; C034693:ethylene forming enzyme