A general method for the detection and quantitation of antigens in solubilized cells using radiolabeled Fab fragment.
Harford
J B
JB
Cell Biology & Metabolism Branch, National Institute of Child Health & Human Development, National Institutes of Health, Bethesda, Maryland 20892.
D000645:Ammonium Sulfate / Q000737:chemistry; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000941:Antigens / Q000032:analysis*; D002460:Cell Line; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000276:immunology; D011233:Precipitin Tests; D011863:Radioimmunoassay; D011990:Receptors, Transferrin / Q000032:analysis*; D012995:Solubility
D000911:Antibodies, Monoclonal; D000941:Antigens; D007140:Immunoglobulin Fab Fragments; D011990:Receptors, Transferrin; D000645:Ammonium Sulfate