A strategy for production of monoclonal antibodies to Echinococcus granulosus antigen 5 and antigen B.
Serum antibody responses to sheep hydatid cyst fluid (SHCF) and a purified Antigen 5 (Ag5) were examined in ELISA, immunoelectrophoresis (IEP) and immunoprecipitation (IP) to facilitate production of monoclonal antibodies (MAb) to E. granulosus Ag5 and Antigen B (AgB). Although sera from mice immunized with SHCF contained antibodies of various classes, the fusions using these donor mice resulted in mainly anti-AgB MAb, possibly due to the preferential selection of MAb to AgB by the SHCF-based ELISA screening system. Donor mice immunized with Ag5 also produced several classes of antibodies, and the resultant fusions enabled selection of IgG MAb to Ag5.
Liu
D
D
University of Melbourne, Veterinary Clinical Centre, Werribee, Victoria, Australia.
Rickard
M D
MD
Lightowlers
M W
MW
D000818:Animals; D000909:Antibodies, Helminth / Q000096:biosynthesis*; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D000947:Antigens, Helminth / Q000276:immunology*; D004446:Echinococcus / Q000276:immunology*; D005260:Female; D006825:Hybridomas; D008297:Male; D051379:Mice; D012756:Sheep
D000909:Antibodies, Helminth; D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth