[Pepsin in the enzymatic synthesis of esters and n-nitroanilide peptides].
Pepsin was shown to catalyze synthesis of esters or p-nitroanilides tri-, tetra-, penta- and hexapeptides of general formula Z-X-Y-B, where X = Ala-Phe, Phe-Met, Ala-Ala-Glu, Ala-Ala-Phe, Ala-Ala-Leu, Ala-Ala-Trp, Ala-Ala-Met. Y = Ala, Leu, Val, Phe, Arg, Ala-Ala, Gly-Gly, Leu-Ala-Ala, Phe-Ala-Ala. B = OMe, pNA. The reactions were carried out in dimethylformamide-water solutions at pH 4.6 by equimolar ratio of amino- and carboxyl components (with the exception of Arg-pNA taken in 2-fold excess). The amount of pepsin in the reaction approached 1:1700 enzyme: substrate molar ratio although it might be improved--up to 1:3.10(5) for relatively long peptides.
Lysogorskaia
E N
EN
Filippova
I Iu
IIu
Abdel' Malak
K A
KA
Lavrenova
G I
GI
Anisimova
V V
VV
Oksenoĭt
E S
ES
Stepanov
V M
VM
D000595:Amino Acid Sequence; D000813:Anilides / Q000737:chemistry*; D000818:Animals; D002384:Catalysis; D004952:Esters / Q000737:chemistry; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000138:chemical synthesis*; D010434:Pepsin A / Q000737:chemistry*; D010455:Peptides / Q000138:chemical synthesis*; D013552:Swine
D000813:Anilides; D004952:Esters; D009842:Oligopeptides; D010455:Peptides; D010434:Pepsin A
Pepsin v fermentativnom sintexze éfirov i n-nitroanilidov peptidov.