[Expansion of CD4+CD8+ T lymphocytes in the peripheral blood of a patient with pulmonary tuberculosis].
We detected a high level of CD4+CD8+ double marker cells in the peripheral blood of a male patient with pulmonary tuberculosis (TB), ranging from 10% to 12%. Three color staining was useful for the further examination of surface markers of these abnormal lymphocytes. Three color staining of his CD4+CD8+ cells demonstrated that his CD4+CD8+ cells expressed CD2, CD3 and TCR alpha beta. Stimulation of his PBMC with OKT3 (or rIL-2) for 4 days resulted in the increase of up to about 50% (20%) of CD4+CD8+ cells. His PBMC had a normal proliferative response to mitogen. Although we could not evaluate the significance of the presence of an expanded CD4+CD8+ cells, it was not correlate with the disease of TB or the activity of the disease at least.
Ohnishi
K
K
Osaka Prefectural Habikino Hospital.
D000954:Antigens, Surface / Q000032:analysis; D016516:CD4-CD8 Ratio; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D008875:Middle Aged; D014397:Tuberculosis, Pulmonary / Q000276:immunology*
D000954:Antigens, Surface