Establishment of a folliculo-stellate-like cell line from a murine thyrotropic pituitary tumor.
An isologously transplantable mouse thyrotropic pituitary tumor (TtTb) that had been induced by radiothyroidectomy was found to form lobules composed of parenchymal glandular cell elements. The surfaces of these lobules were covered by stellate cells bearing long processes that gave a strong immunopositive reaction with a specific antiserum against glial fibrillary acidic protein (GFAP). GFAP was also positive in pituitary folliculo-stellate cells (FS cells). Since in the anterior pituitary gland, no other cellular component showing positive staining with anti-GFAP antibody was observed, it seems probable that the GFAP-positive cells in the thyrotropic tumor are related to the FS cells in the anterior pituitary. Upon cultivation of the thyrotropic tumor, cells with long processes appeared. These cells showed strong staining for GFAP and were dependent on basic fibroblast growth factor for cell growth. By repeated passage, the GFAP-positive cells became a stably growing immortal cell line, which was tentatively named TtT/GF. Since the characteristics of TtT/GF were similar to those of FS cells in the anterior pituitary, i.e. the presence of many lysosomes and numerous intermediate filaments in the cytoplasm, phagocytic activity, follicle formation, and GFAP and S-100 protein positivity, we suggest that TtT/GF cells are derived from pituitary FS cells.
Inoue
K
K
Department of Morphology, Gunma University School of Medicine, Maebashi, Japan.
Matsumoto
H
H
Koyama
C
C
Shibata
K
K
Nakazato
Y
Y
Ito
A
A
D000818:Animals; D015153:Blotting, Western; D003470:Culture Media; D003593:Cytoplasm / Q000737:chemistry; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D005455:Fluorescent Antibody Technique; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D051379:Mice; D008854:Microscopy, Electron; D010903:Pituitary Gland, Anterior / Q000473:pathology*; D010911:Pituitary Neoplasms / Q000473:pathology*; D009418:S100 Proteins / Q000032:analysis; D013972:Thyrotropin / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D003470:Culture Media; D005904:Glial Fibrillary Acidic Protein; D009418:S100 Proteins; D016222:Fibroblast Growth Factor 2; D013972:Thyrotropin