Effect of liposomes on lipid peroxidation and total phospholipids in rabbit ischemic spinal cord model.
The effect of spinal cord ischemia (induced by abdominal aorta ligation for 20 minutes) on lipid peroxidation and TPL composition was investigated and discussed in our previous articles. It is known, that partially reduced species of oxygen can be formed under aerobic conditions. For that reason, the effect of ligation release for 60 minutes was observed in experimental animals treated with the selected liposomes. Administration of CP, (CP+SA) and (CP+Chol) liposomes applied 30 minutes before 20 minutes ischemia revealed an ameliorating effect on in vivo and in vitro Fe-dependent peroxidation manifested by TBA-RS accumulation. Combined use of (CP+SA) liposomes with lipophylic form of stobadine (DP 1031) was not more effective. Application of CP liposomes directly before the ligation release slightly increased the antiradical capacity in spinal cord homogenates comparing with not-treated animals. Accumulation of TBA-RS was accompanied by TPL degradation during recirculation period but values of TPL after liposomal treatment were unaffected.
Lukácová
N
N
Institute of Neurobiology, Slovak Academy of Sciences, Srobárova, Kosice.
Halát
G
G
Briginskij
S
S
Marsala
J
J
D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D004195:Disease Models, Animal; D007511:Ischemia / Q000378:metabolism / Q000517:prevention & control*; D015227:Lipid Peroxidation / Q000187:drug effects*; D008081:Liposomes / Q000494:pharmacology*; D008297:Male; D010743:Phospholipids / Q000302:isolation & purification / Q000378:metabolism*; D011817:Rabbits; D013116:Spinal Cord / Q000098:blood supply / Q000187:drug effects*
D008081:Liposomes; D010743:Phospholipids