Stimulation of glucose transport by guanine nucleotides in permeabilized rat adipocytes.
Effects of guanine nucleotides on glucose transport were studied in permeabilized rat epididymal fat cells. GTP gamma S and Gpp(NH)p, but not App(NH)p, stimulated 3-O-methylglucose transport. Effect of GTP gamma S was dose-dependent, being detectable at 0.1 mM, and 1.0 mM GTP gamma S stimulated glucose transport to the same extent as insulin. GTP gamma S (0.3 mM) enhanced insulin-stimulated glucose transport while 1 mM GTP gamma S did not affect insulin-mediated transport. GDP beta S had no effect on glucose transport by itself but rather enhanced insulin action. NaF, which is known to activate trimeric G proteins, increased glucose transport to the same extent as insulin. Likewise, mastoparan augmented glucose transport. These results indicate that a certain type of trimeric G protein(s) is involved in the regulation of glucose transport.
Suzuki
Y
Y
Cell Biology Research Unit Institute of Endocrinology, Gunma University, Japan.
Shibata
H
H
Inoue
S
S
Kojima
I
I
D019325:3-O-Methylglucose; D000266:Adenylyl Imidodiphosphate / Q000494:pharmacology; D000273:Adipose Tissue / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002463:Cell Membrane Permeability; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004558:Electric Stimulation; D005947:Glucose / Q000378:metabolism*; D006150:Guanine Nucleotides / Q000494:pharmacology*; D016244:Guanosine 5'-O-(3-Thiotriphosphate) / Q000494:pharmacology; D006153:Guanosine Diphosphate / Q000031:analogs & derivatives / Q000494:pharmacology; D006165:Guanylyl Imidodiphosphate / Q000494:pharmacology; D007328:Insulin / Q000494:pharmacology; D036341:Intercellular Signaling Peptides and Proteins; D008297:Male; D008757:Methylglucosides / Q000378:metabolism*; D010455:Peptides; D051381:Rats; D017207:Rats, Sprague-Dawley; D012969:Sodium Fluoride / Q000494:pharmacology; D013873:Thionucleotides / Q000494:pharmacology; D014862:Wasp Venoms / Q000494:pharmacology
D006150:Guanine Nucleotides; D007328:Insulin; D036341:Intercellular Signaling Peptides and Proteins; D008757:Methylglucosides; D010455:Peptides; D013873:Thionucleotides; D014862:Wasp Venoms; D019325:3-O-Methylglucose; D006153:Guanosine Diphosphate; D000266:Adenylyl Imidodiphosphate; D006165:Guanylyl Imidodiphosphate; D016244:Guanosine 5'-O-(3-Thiotriphosphate); C023427:guanosine 5'-O-(2-thiodiphosphate); C037500:mastoparan; D012969:Sodium Fluoride; D005947:Glucose