Platelet dense granule release reaction monitored by high-performance liquid chromatography-fluorometric determination of endogenous serotonin.
The platelet dense granule release reaction was monitored by measuring the serotonin content of platelets collected by filtration following release. Sensitive HPLC-fluorometric analysis permitted release to be assessed using small (25-50 microliters) volumes of plasma and whole blood. Release could be examined for periods as short as 1 s due to the rapid stoppage effected by filtration. The half-life of thrombin-stimulated (1 U/ml) release was 2-3 s, the EC50 values observed for thrombin-stimulated release over a 60-s release period were 0.01-0.03 U/ml for diluted whole blood and plasma samples.
Anderson
G M
GM
Child Study Center, Yale University School of Medicine, New Haven, Connecticut 06510.
Hall
L M
LM
Yang
J X
JX
Cohen
D J
DJ
D001792:Blood Platelets / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003594:Cytoplasmic Granules / Q000187:drug effects / Q000378:metabolism*; D005374:Filtration; D005470:Fluorometry / Q000379:methods; D005473:Fluoxetine / Q000494:pharmacology; D006801:Humans; D007700:Kinetics; D012701:Serotonin / Q000097:blood* / Q000378:metabolism; D013268:Stimulation, Chemical; D013917:Thrombin / Q000494:pharmacology
D005473:Fluoxetine; D012701:Serotonin; D013917:Thrombin