Relative abilities of distinct isotypes of human major histocompatibility complex class II molecules to bind streptococcal pyrogenic exotoxin types A and B.
The relative ability of distinct isotypes of human leukocyte antigen class II molecules to bind streptococcal pyrogenic exotoxins A and B (SPE A and SPE B, respectively) was investigated by a direct-binding assay with 125I-labeled toxin for SPE A and by a functional assay system measuring the accessory cell activity of human leukocyte antigen class II transfectants in toxin-induced T-cell activation for SPE A and SPE B. SPE A binding was observed in L cells transfected with DQw1 genes. By contrast, it was not detected in L cells transfected with DR2, DR4, DPw4 or DP(Cp63) genes. All the transfectants supported SPE-induced interleukin-2 production by human T cells except the DP transfectants for SPE B. Levels of accessory cell activity were low in the DP transfectants induced by stimulation with SPE A and in the DR and DP transfectants induced by SPE B. The results indicate that SPE A and SPE B bind well to DQ molecules, less well to DR molecules, and very weakly to DP molecules.
Imanishi
K
K
Department of Microbiology and Immunology, Tokyo Women's Medical College, Japan.
Igarashi
H
H
Uchiyama
T
T
D000818:Animals; D000938:Antigen-Presenting Cells / Q000502:physiology; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004768:Enterotoxins / Q000276:immunology / Q000378:metabolism; D005098:Exotoxins / Q000378:metabolism*; D005802:Genes, MHC Class II; D000949:Histocompatibility Antigens Class II / Q000378:metabolism*; D006801:Humans; D007376:Interleukin-2 / Q000096:biosynthesis; D007739:L Cells; D008213:Lymphocyte Activation; D008565:Membrane Proteins; D051379:Mice; D013297:Streptococcus pyogenes / Q000378:metabolism*; D018089:Superantigens; D014162:Transfection
D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004768:Enterotoxins; D005098:Exotoxins; D000949:Histocompatibility Antigens Class II; D007376:Interleukin-2; D008565:Membrane Proteins; C117980:SpeA protein, Streptococcus pyogenes; D018089:Superantigens; C030107:enterotoxin F, Staphylococcal; C027951:erythrogenic toxin