title: The effect of metal binding on the structure of annexin V and implications for membrane binding.
abstract: The structure of annexin V, crystallised in the presence of two calcium or barium ions for each protein molecule, was solved by molecular replacement to 0.24 nm resolution. The two metal ions are found in domains I and IV, i.e. on the same side of the channel that lies in the centre of the molecule. The structures of the barium and calcium form are extremely close, the only differences localised in the metal-binding sites that lie on the surface of the molecule. The occupancies of the metal ions, however, are lower for barium than for calcium, expressing the lower affinity of the protein for the former. The packing of the annexin molecules in the crystal asymmetric unit may represent a model for the calcium driven association of membrane-bound annexins that leads to membrane fusion.
authors.affiliation: Laboratoire pour l'Utilisation du Rayonnement Electromagnetique, Centre Universitaire Paris Sud, Orsay, France.
authors.forename: A
authors.initials: A
authors.lastname: Lewit-Bentley
authors.forename: S
authors.initials: S
authors.lastname: Morera
authors.forename: R
authors.initials: R
authors.lastname: Huber
authors.forename: G
authors.initials: G
authors.lastname: Bodo
mesh_terms: D017304:Annexin A5 / Q000737:chemistry / Q000378:metabolism*; D001464:Barium / Q000378:metabolism*; D001665:Binding Sites; D002118:Calcium / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D003460:Crystallization; D006801:Humans; D009994:Osmolar Concentration; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D015032:Zinc / Q000378:metabolism*
chemical_list: D017304:Annexin A5; D011994:Recombinant Proteins; D001464:Barium; D015032:Zinc; D002118:Calcium