New chromosomal mapping assignments for argininosuccinate synthetase pseudogene 1, interferon-beta 3 gene, and the diazepam binding inhibitor gene.
Argininosuccinate synthetase pseudogene 1 (ASSP1), interferon-beta 3 (IFNB3) gene, and diazepam binding inhibitor (DBI) gene have previously been mapped to human chromosome 2. Their nucleotide sequences, recorded in the GENBANK data base, were used to generate DNA primers to amplify specific sequences using the polymerase chain reaction (PCR). These primers failed to amplify DNA sequences when used to analyze microcell hybrid clones containing human chromosome 2. In order to map these genes, a panel of somatic cell hybrids was analyzed by PCR with these primer sets. The results of these experiments place ASSP1 sequences on human chromosome 6, IFNB3 on human chromosome 8, and DBI on human chromosome 6.
Todd
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Department of Cellular and Structural Biology, University of Texas Health Science Center, San Antonio 78284-7762.
Naylor
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SL
D001124:Argininosuccinate Synthase / Q000235:genetics*; D001483:Base Sequence; D002874:Chromosome Mapping; D002877:Chromosomes, Human; D002896:Chromosomes, Human, Pair 6; D002898:Chromosomes, Human, Pair 8; D026261:Diazepam Binding Inhibitor; D006801:Humans; D016899:Interferon-beta / Q000235:genetics*; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011544:Pseudogenes / Q000235:genetics*
D026261:Diazepam Binding Inhibitor; D009479:Neuropeptides; D016899:Interferon-beta; D001124:Argininosuccinate Synthase