Structure of hypothalamic coronaro-constrictory peptide factors.
The following peptide structure in 3 of 5 coronaro-constrictory peptide factors isolated from bovine hypothalamus was determined by amino acid analysis and Edman degradation: 1) (P1)--Val-Val-Tyr-Pro-Trp; 2) (P2)--Val-Val-Tyr-Pro-Trp-Thr; 3) (P3)--Leu-Val-Val-Tyr-Pro-Trp-Thr. A computer search for these amino acid sequences revealed that these peptides represent fragments 33-37; 33-38; 32-38 of the beta-chain of bovine hemoglobin. Solid phase peptide synthesis of 2 peptides (P2 and P3) was carried out. It was established that synthetic peptides had the properties of coronaro-constrictory peptides. The possibility of the formation of hypothalamic coronaro-constrictory peptides in vivo is discussed.
Barkhudaryan
N
N
Institute of Biochemistry Armenian Academy of Science, Yerevan.
Oberthuer
W
W
Lottspeich
F
F
Galoyan
A
A
D000595:Amino Acid Sequence; D000818:Animals; D002147:Calmodulin / Q000378:metabolism; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D003331:Coronary Vessels / Q000502:physiology; D004789:Enzyme Activation / Q000187:drug effects; D004797:Enzyme-Linked Immunosorbent Assay; D006454:Hemoglobins / Q000737:chemistry; D006801:Humans; D007031:Hypothalamus / Q000737:chemistry*; D008969:Molecular Sequence Data; D009132:Muscles / Q000201:enzymology; D009219:Myosin-Light-Chain Kinase / Q000378:metabolism; D009479:Neuropeptides / Q000737:chemistry* / Q000494:pharmacology; D010446:Peptide Fragments / Q000737:chemistry; D010974:Platelet Aggregation / Q000187:drug effects; D014661:Vasoconstriction
D002147:Calmodulin; D006454:Hemoglobins; D009479:Neuropeptides; D010446:Peptide Fragments; D009219:Myosin-Light-Chain Kinase