[Polypeptides p14 and p31 of the African swine fever virus--early proteins located on the membrane of the infected cell].
African swine fever virus polypeptides p14 and p31 are synthesized in the presence of phosphonacetic acid which inhibits viral DNA replication, and therefore they are early viral proteins. These polypeptides were found to be localized on plasma membranes by immunofluorescence with monospecific antisera and monoclonal antibodies and by selective solubilization of infected cells. The p14-specific antibodies mediate complement-dependent cytolysis and antibody-dependent cytotoxicity of the cells infected with African swine fever virus.
Kolontsov
A A
AA
Ustin
A V
AV
Shubina
N G
NG
Piria
A A
AA
Makarov
V V
VV
D000357:African Swine Fever / Q000276:immunology / Q000378:metabolism* / Q000382:microbiology; D000358:African Swine Fever Virus / Q000737:chemistry* / Q000276:immunology; D000818:Animals; D000920:Antibody-Dependent Cell Cytotoxicity; D002478:Cells, Cultured / Q000276:immunology / Q000378:metabolism / Q000382:microbiology; D015941:Complement Hemolytic Activity Assay; D004591:Electrophoresis, Polyacrylamide Gel; D005455:Fluorescent Antibody Technique; D015151:Immunoblotting; D008565:Membrane Proteins / Q000032:analysis* / Q000276:immunology / Q000302:isolation & purification; D008970:Molecular Weight; D010455:Peptides / Q000032:analysis* / Q000276:immunology / Q000302:isolation & purification; D011817:Rabbits; D013552:Swine; D013997:Time Factors; D014764:Viral Proteins / Q000032:analysis* / Q000276:immunology / Q000302:isolation & purification
D008565:Membrane Proteins; D010455:Peptides; D014764:Viral Proteins
Polipeptidy p14 i p31 virusa afrikanskoĭ chumy svineĭ--rannie belki, lokalizovannye na membrane infitsirovannoi kletki.