The effects of tumour necrosis factor on host-parasite relations in murine Mesocestoides corti (Cestoda) infection.
The regulatory role of tumour necrosis factor (TNF) was investigated in murine infection with tetrathyridia of Mesocestoides corti. Recombinant TNF alpha reduced macrophage larvicidal activity in vitro. M. corti primed mice for TNF release in response to bacterial lipopolysaccharide (LPS) in vivo. TNF activity was amplified 100-fold at 14 days post-infection (p.i.), with a further rise at day 28 p.i. Maximal inflammatory reaction was observed histologically in the liver at the height of TNF activity. Hepatic necrosis was located within inflammatory foci, but not within the vicinity of the parasite itself, suggesting that TNF may contribute to the pathogenesis of infection. Peritoneal cells from infected mice, when stimulated with tetrathyridia in vitro, showed a 4-fold increase in TNF alpha activity at day 14 p.i. However, when peritoneal cells were stimulated with LPS in vitro, a marked increase in TNF alpha secretion was observed at 2 months post-infection followed by a slow decline. It is suggested that impaired macrophage effector function, previously attributed to endogenous endotoxin, which gains access to peritoneal macrophages through an inability of the liver to detoxify endotoxin, may be mediated through TNF alpha.
Jenkins
P
P
Department of Veterinary Pathology, University of Liverpool.
Spiers
S
S
Dixon
J B
JB
Carter
S D
SD
May
S
S
D000818:Animals; D002478:Cells, Cultured; D002590:Cestode Infections / Q000276:immunology / Q000469:parasitology*; D006790:Host-Parasite Interactions; D007249:Inflammation; D007371:Interferon-gamma / Q000276:immunology; D007814:Larva / Q000276:immunology; D008099:Liver / Q000469:parasitology / Q000473:pathology; D008264:Macrophages / Q000276:immunology; D008645:Mesocestoides / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009336:Necrosis; D011994:Recombinant Proteins; D013047:Specific Pathogen-Free Organisms; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis / Q000502:physiology*
D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma