[Isolation and identification of a strain of Pseudomonas SP. producing insoluble glucan hydrolase].
A strain, CIB871, isolated from the natural world can hydrolyze insoluble glucan(IG) produced by S. mutans. This organism has been identified to be a strain of Pseudomonas SP. It grows well at 30 degrees C. The optimum medium for producing insoluble glucan hydrolase (IGase) is composed of 0.3% peptone, 0.03% IG and M1 salt solution (NH4)2 SO4 1mg/ml, MgSO4 50 micrograms/ml, FeCl2 50 micrograms/ml, K2HPO4 0.5mg/ml). The IGase production reached maximum when cultured for 65-70 hours.
He
G
G
Chengdu Institute of Biology, Academia Sinica.
Yin
S
S
Liu
Y
Y
Shi
Y
Y
Xu
H
H
Zhao
X
X
Pu
Q
Q
Zhang
Y
Y
D005936:Glucans / Q000378:metabolism*; D006026:Glycoside Hydrolases / Q000096:biosynthesis*; D011549:Pseudomonas / Q000201:enzymology* / Q000302:isolation & purification*
D005936:Glucans; D006026:Glycoside Hydrolases