Fish species identification by peptide mapping of the myosin heavy chain.
Fish species of seafood made of washed fish flesh (e.g. imitation crab meat from surimi) were identified by peptide mapping of the myosin heavy chain (MHC). In the first step the MHC was separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) in 7.5% T gel, followed by a second SDS-PAGE in a 15% T gel with proteolytic digestion of the MHC in the gel. The resulting peptides gave species specific patterns.
Rehbein
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Institut für Biochemie und Technologie, Bundesforschungsanstalt für Fischerei, Hamburg, Germany.
D000818:Animals; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D005069:Evaluation Studies as Topic; D005396:Fish Products / Q000032:analysis*; D005399:Fishes / Q000145:classification*; D009218:Myosins / Q000302:isolation & purification*; D010449:Peptide Mapping / Q000379:methods*; D013045:Species Specificity
D009218:Myosins