Evaluation of antifungal activity of antimycotics by automatic analyzing system.
Antifungal activity of several antimycotics has been evaluated using an automatic analyzing system (AAS), which is composed of a specially designed reaction vessel, microscopic observation system, image analyzing system, and computer program for automatic tracing of hypha growth. The agar plate was prepared on the ceiling of the reaction vessel, and spore mass of a fungus (Aspergillus niger) was inoculated onto it. After the preincubation at 28 degrees C for 24 h the reaction vessel was set on a microscope stage and connected to the liquid flow system. An appropriate hypha was selected for the measurement of growth process during the following steps: first contact with saline for 30 min for the adaptation, the second contact with same saline for 30 min, contact with saline containing an antimycotic substance for 60 min, and contact with flushing saline for 60 min. During a sequence of these steps, the apical tip of a growing hypha displayed on a TV monitor was followed automatically. The dynamic response of hypha to an agent was analyzed by several parameters. Morphological changes of the hypha caused by respective agents were recorded on VTR for further analysis. By using this system, the antifungal activity of antimycotics could be quantitatively determined within several hours.
Oh
K
K
Department of Biotechnology, Faculty of Technology, Tokyo University of Agriculture and Technology, Japan.
Matsuoka
H
H
Sumita
O
O
Takatori
K
K
Kurata
H
H
D000666:Amphotericin B / Q000494:pharmacology; D000935:Antifungal Agents / Q000494:pharmacology*; D000968:Antimycin A / Q000494:pharmacology; D001234:Aspergillus niger / Q000187:drug effects* / Q000254:growth & development; D000077768:Ciclopirox; D005437:Flucytosine / Q000494:pharmacology; D005658:Fungi / Q000187:drug effects* / Q000254:growth & development; D006118:Griseofulvin / Q000494:pharmacology; D007091:Image Processing, Computer-Assisted; D007654:Ketoconazole / Q000494:pharmacology; D009761:Nystatin / Q000494:pharmacology; D011728:Pyridones / Q000494:pharmacology; D012965:Sodium Chloride / Q000494:pharmacology
D000935:Antifungal Agents; D011728:Pyridones; D009761:Nystatin; D000077768:Ciclopirox; D006118:Griseofulvin; D012965:Sodium Chloride; D000968:Antimycin A; D000666:Amphotericin B; D005437:Flucytosine; D007654:Ketoconazole