title: C-type natriuretic peptide in bovine chromaffin cells. The regulation of its biosynthesis and secretion.
abstract: We report here the regulation of the biosynthesis and the secretion of C-type natriuretic peptide (CNP) in cultured bovine chromaffin cells. The combined treatment with protein kinase A and -C activators induced a 6-fold increase of intracellular levels of CNP-(1-103). The biosynthesized CNP-(1-103) was co-released with its mature forms, typically CNP-(51-103), upon stimulation by nicotine or depolarizing agents. This confirms the neuropeptidic character of this third member of the natriuretic peptide family, which might act as a neuromodulator or neurotransmitter.
authors.affiliation: Department of Pharmacology, Faculty of Medicine, University of Montréal, Qué, Canada.
authors.forename: K
authors.initials: K
authors.lastname: Babinski
authors.forename: J
authors.initials: J
authors.lastname: Féthière
authors.forename: M
authors.initials: M
authors.lastname: Roy
authors.forename: A
authors.initials: A
authors.lastname: De Léan
authors.forename: H
authors.initials: H
authors.lastname: Ong
mesh_terms: D000313:Adrenal Medulla / Q000378:metabolism*; D000818:Animals; D009320:Atrial Natriuretic Factor / Q000378:metabolism; D002417:Cattle; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D005576:Colforsin / Q000494:pharmacology; D066298:In Vitro Techniques; D020098:Natriuretic Peptide, C-Type; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D011493:Protein Kinase C / Q000378:metabolism; D011494:Protein Kinases / Q000378:metabolism; D011498:Protein Precursors / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
chemical_list: D009419:Nerve Tissue Proteins; D011498:Protein Precursors; D020098:Natriuretic Peptide, C-Type; D005576:Colforsin; D009320:Atrial Natriuretic Factor; D011494:Protein Kinases; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate