The effect of calcium on disodium monofluorophosphate absorption from the gastrointestinal tract of rats.
The absorption of fluoride from disodium monofluorophosphate with or without added calcium has been studied in ligated stomachs and duodena of rats, in vivo. Measurements of fluoride absorption from sodium fluoride were also carried out for comparative purposes. The formation constant of the soluble, neutral calcium monofluorophosphate complex has been determined at 20 degrees, 25 degrees and 37 degrees C. Its value at 37 degrees C being 315 +/- 10 (molar units). The influence of increasing concentrations of calcium on alkaline phosphatase (E.C.3.1.3.1) activity with disodium monofluorophosphate as substrate has been also studied. Gastric absorption of 2 mM disodium monofluorophosphate in the presence of 50 mM calcium was much slower than that of 2 mM disodium monofluorophosphate alone. The latter was slower than that of 2 mM sodium fluoride. The opposite situation has been found for the duodenal fluoride absorption. Results obtained are interpreted in terms of the occurrence of an intestinal monofluorophosphate hydrolysis prior to its absorption as fluoride. In addition, data presented suggest an independent and parallel pathway of fluoride absorption in the form of the liposoluble calcium monofluorophosphate complex.
Villa
A
A
Unidad de Bioquímica Farmacológica y Lípidos, INTA, Universidad de Chile, Santiago.
Carrasco
G
G
Valenzuela
A
A
Garrido
A
A
D000042:Absorption / Q000187:drug effects; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000494:pharmacology*; D004386:Duodenum / Q000187:drug effects* / Q000378:metabolism; D004566:Electrodes; D005260:Female; D005459:Fluorides / Q000493:pharmacokinetics*; D005753:Gastric Mucosa / Q000378:metabolism; D006868:Hydrolysis; D007408:Intestinal Absorption / Q000187:drug effects*; D010710:Phosphates / Q000493:pharmacokinetics*; D051381:Rats; D017208:Rats, Wistar; D012969:Sodium Fluoride / Q000493:pharmacokinetics; D013270:Stomach / Q000187:drug effects*
D010710:Phosphates; C012980:fluorophosphate; D012969:Sodium Fluoride; D000469:Alkaline Phosphatase; D005459:Fluorides; D002118:Calcium