title: [Cryopreservation of spermatozoa of a transgenic mouse].
abstract: Spermatozoa of a homozygous transgenic mouse, in which the firefly luciferase gene was expressed under the control of beta-actin promoter, were frozen at -196 degrees C. One fourth of the frozen sperm was later thawed and used for in vitro fertilization. Thirty-six of 65 oocytes (55.4%) developed to the 2-cell stage. All the 2-cell embryos were transferred to the oviducts of pseudopregnant recipients and 23 young (63.9%, 23/36) were born. All of young analyzed carried the transgene and showed the luciferase gene expression.
authors.affiliation: NT Science Co., Ltd., Tokyo, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Nakagata
authors.forename: K
authors.initials: K
authors.lastname: Matsumoto
authors.forename: M
authors.initials: M
authors.lastname: Anzai
authors.forename: A
authors.initials: A
authors.lastname: Takahashi
authors.forename: Y
authors.initials: Y
authors.lastname: Takahashi
authors.forename: Y
authors.initials: Y
authors.lastname: Matsuzaki
authors.forename: K
authors.initials: K
authors.lastname: Miyata
mesh_terms: D000199:Actins; D000818:Animals; D015925:Cryopreservation / Q000662:veterinary*; D004624:Embryo Transfer; D005307:Fertilization in Vitro; D015971:Gene Expression Regulation, Enzymologic; D008156:Luciferases / Q000235:genetics*; D008297:Male; D051379:Mice; D008822:Mice, Transgenic / Q000235:genetics*; D012098:Reproduction; D013094:Spermatozoa
chemical_list: D000199:Actins; D008156:Luciferases