Isolation and characterization of cDNA encoding a spicule matrix protein in Hemicentrotus pulcherrimus micromeres.
A cDNA clone, termed pHPSMC, was obtained from the Japanese sea urchin Hemicentrotus pulcherrimus, and it was found to be highly homologous in sequence to the spicule matrix protein cDNA of Strongylocentrotus purpuratus (Sucov et al., Dev. Biol. 120: 507-519, 1987). During early embryogenesis, mRNA complementary to pHPSMC appeared in gastrulae and remained at a similar level until the pluteus stage. In situ hybridization revealed that the mRNA was localized exclusively in primary mesenchyme cells in gastrulae. pHPSMC mRNA was detected in micromeres in vitro after 48 h of culture, but it was not found in blastomeres immediately after isolation. These features suggested that pHPSMC represents the spicule matrix protein cDNA cognate in Hemicentrotus pulcherrimus. In the derived polypeptide, we detected a domain containing a tandemly repeated 13-amino acid sequence as did Sucov et al. (1987). Unexpectedly, the sequence of the repeated element was completely different from that originally reported for Strongylocentrotus purpuratus, but it was very similar to the corrected sequence that appeared recently.
Katoh-Fukui
Y
Y
Department of Developmental Biology, Mitsubishi Kasei Institute of Life Sciences, Tokyo, Japan.
Noce
T
T
Ueda
T
T
Fujiwara
Y
Y
Hashimoto
N
N
Tanaka
S
S
Higashinakagawa
T
T
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D016384:Consensus Sequence; D003598:Cytoskeletal Proteins; D004247:DNA / Q000302:isolation & purification*; D016326:Extracellular Matrix Proteins / Q000235:genetics*; D005775:Gastrula / Q000378:metabolism*; D008969:Molecular Sequence Data; D016372:RNA, Antisense; D012617:Sea Urchins / Q000196:embryology / Q000235:genetics*
D003598:Cytoskeletal Proteins; D016326:Extracellular Matrix Proteins; D016372:RNA, Antisense; C051691:SM50 protein, sea urchin; D004247:DNA