Mannan-coated liposome delivery of gadolinium-diethylenetriaminepentaacetic acid, a contrast agent for use in magnetic resonance imaging.
Gadolinium-diethylenetriaminepentaacetic acid (Gd-DTPA), a paramagnetic contrast agent for use in magnetic resonance imaging (MRI) was bound to stearylamine and incorporated into the liposomal membranes (Gd-DTPA liposomes). In addition, the Gd-DTPA liposomes were coated with mannan (cholesterol-aminoethylcarbamylmethyl mannan), a polysaccharide, to obtain the mannan-coated liposomes. An in vitro MRI study showed that the Gd-DTPA liposomes produced a greater intensity of contrast than did the Gd-DTPA solution with a reduced T1 relaxation time. Intravenous injection of the Gd-DTPA liposomes containing 153Gd or liposomes containing 153Gd or 14C-DTPA to mice showed an accumulation of Gd-DTPA primarily in the liver and lung. When the mannan-coated liposomes were administered, an increased uptake of Gd-DTPA by these tissues was demonstrated. The mannan-coated liposomes may enhance contrast of the liver in MRI at a lower dose of Gd-DTPA.
Kunimasa
J
J
Department of Pharmacy, Kyoto University Hospital, Japan.
Inui
K
K
Hori
R
R
Kawamura
Y
Y
Endo
K
K
D000818:Animals; D003287:Contrast Media; D005682:Gadolinium; D019786:Gadolinium DTPA; D008081:Liposomes; D008279:Magnetic Resonance Imaging; D051379:Mice; D009942:Organometallic Compounds / Q000008:administration & dosage / Q000493:pharmacokinetics*; D004369:Pentetic Acid / Q000008:administration & dosage / Q000493:pharmacokinetics*; D011868:Radioisotopes; D014018:Tissue Distribution
D003287:Contrast Media; D008081:Liposomes; D009942:Organometallic Compounds; D011868:Radioisotopes; D004369:Pentetic Acid; D005682:Gadolinium; D019786:Gadolinium DTPA