Expression of a coriander desaturase results in petroselinic acid production in transgenic tobacco.
Little is known about the metabolic origin of petroselinic acid (18:1 delta 6cis), the principal fatty acid of the seed oil of most Umbelliferae, Araliaceae, and Garryaceae species. To examine the possibility that petroselinic acid is the product of an acyl-acyl carrier protein (ACP) desaturase, Western blots of coriander and other Umbelliferae seed extracts were probed with antibodies against the delta 9-stearoyl-ACP desaturase of avocado. In these extracts, proteins of 39 and 36 kDa were detected. Of these, only the 36-kDa peptide was specific to tissues which synthesize petroselinic acid. A cDNA encoding the 36-kDa peptide was isolated from a coriander endosperm cDNA library, placed under control of the cauliflower mosaic virus 35S promoter, and introduced into tobacco by Agrobacterium tumefaciens-mediated transformation. Expression of this cDNA in transgenic tobacco callus was accompanied by the accumulation of petroselinic acid and delta 4-hexadecenoic acid, both of which were absent from control callus. These results demonstrate the involvement of a 36-kDa putative acyl-ACP desaturase in the biosynthetic pathway of petroselinic acid and the ability to produce fatty acids of unusual structure in transgenic plants by the expression of the gene for this desaturase.
Cahoon
E B
EB
Department of Botany and Plant Pathology, Michigan State University, East Lansing 48824.
Shanklin
J
J
Ohlrogge
J B
JB
D000595:Amino Acid Sequence; D001483:Base Sequence; D015153:Blotting, Western; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D004926:Escherichia coli; D015870:Gene Expression; D005796:Genes; D013058:Mass Spectrometry; D006899:Mixed Function Oxygenases / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D009829:Oleic Acids / Q000096:biosynthesis*; D030821:Plants, Genetically Modified; D010947:Plants, Toxic; D012333:RNA, Messenger / Q000235:genetics; D016415:Sequence Alignment; D017365:Spices; D014026:Nicotiana
D009829:Oleic Acids; D012333:RNA, Messenger; C008820:petroselinic acid; D004247:DNA; D006899:Mixed Function Oxygenases; C018747:acyl-(acyl-carrier-protein)desaturase