title: Histochemistry of glandular metaplasia at the trigone of the urinary bladder in cows.
abstract: The epithelium at the trigone of the urinary bladder showed intestinal metaplasia of a colonic type in three Holstein-Friesian cows affected with chronic polypoid cystitis. Except for Ricinus communis (RCA-I), almost all goblet cells in the whole crypt were positive to periodic acid-Schiff (PAS), alcian blue (AB) pH 2.5, AB pH 1.0, periodate borohydride-potassium hydroxide-PAS (PB-KOH-PAS), Ulex europaeus (UEA-I), Triticum vulgare (WGA) and Arachis hypogaea (PNA) after neuraminidase digestion. This result indicated that most goblet cells contained acidic and neutral glycoconjugates as O-acetylated sialomucin, L-fucose, N-acetyl-glucosamine, neuraminic acid residues and sialic acid-galactose dimers and were devoid of beta-D-galactose. The goblet cells at the surface in the upper half of the crypt contained both sulpho- and sialo-mucins with N-acetyl-galactosamine residues by AB (pH 2.5)-PAS, high iron diamine (HID)-AB (pH 2.5), Dolichos biflorus (DBA) and Glycine maximus (SBA) reactions. On the other hand, the lower goblet cells were found to contain predominantly sulphated mucins with D-mannose and D-glucose residues by AB-PAS, HID-AB and Concanavalia ensiformis (Con A) reactivities. This suggested that mucin secreted from these cells was similar to that secreted from the goblet cells of the large intestine in cattle.
authors.affiliation: Department of Veterinary Pathology, School of Veterinary Medicine, Rakuno Gakuen University, Hokkaido, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Okada
authors.forename: K
authors.initials: K
authors.lastname: Takehana
authors.forename: K
authors.initials: K
authors.lastname: Takahashi
authors.forename: K
authors.initials: K
authors.lastname: Matsukawa
mesh_terms: D000423:Alcian Blue; D000818:Animals; D002240:Carbohydrate Sequence; D002417:Cattle; D002418:Cattle Diseases / Q000473:pathology*; D002908:Chronic Disease; D003106:Colon; D003556:Cystitis / Q000150:complications / Q000473:pathology*; D004848:Epithelium / Q000473:pathology; D005260:Female; D006001:Glycoconjugates / Q000032:analysis; D006869:Hydronephrosis / Q000209:etiology / Q000473:pathology; D007413:Intestinal Mucosa; D037102:Lectins; D008679:Metaplasia; D008969:Molecular Sequence Data; D009077:Mucins / Q000032:analysis; D010503:Periodic Acid-Schiff Reaction; D012795:Sialoglycoproteins / Q000032:analysis; D001743:Urinary Bladder / Q000473:pathology*
chemical_list: D006001:Glycoconjugates; D037102:Lectins; D009077:Mucins; D012795:Sialoglycoproteins; D000423:Alcian Blue