title: Toxicities of microencapsulated tribromomethane, dibromochloromethane and bromodichloromethane administered in the diet to Wistar rats for one month.
abstract: The gelatin-starch syrup microencapsulation method was applied to subacute toxicity studies of tribromomethane (TBM), dibromochloromethane (DBCM) and bromodichloromethane (BDCM). Groups of Wistar rats (7 males and 7 females) both sexes were given diet containing microcapsules of each of these trihalomethanes (THMs) at the following concentrations: TBM, 0.068, 0.204 and 0.612% in males, and 0.072, 0.217 and 0.651% in females; DBCM, 0.020, 0.062 and 0.185% in males, and 0.038, 0.113 and 0.338% in females; BDCM, 0.024, 0.072 and 0.215% in males, and 0.024, 0.076 and 0.227% in females. Suppression of body weight gain was seen in each high-dose males fed TBM or BDCM and females fed DBCM or BDCM. Histopathologically, hepatic lesions such as vacuolization and swelling of liver cells were significantly noted in both sexes of all groups fed TBM, in both sexes of the middle- and high-dose groups fed DBCM, and in males of the high-dose group and in females of the middle- and high-dose groups fed BDCM. In addition, single cell necroses were observed in males and females fed DBCM and in males fed BDCM. Hepatic cord abnormalities were also noted in males of the high-dose group fed BDCM. Although no increases in serum transaminase activities (ASAT, ALAT) were evident in either sex fed any of the THMs, decreases in triglyceride content, cholinesterase and lactate dehydrogenase activity were observed. Renal lesions reported to occur in gavage studies were not found in the present feeding study. Lowest-observed-adverse-effect-level (LOAEL) of TBM and no-observed-adverse-effect-level (NOAEL) of DBCM and BDCM were determined to be 56.4 mg/kg, 18.3 mg/kg and 20.6 mg/kg, respectively, under the present experimental conditions.
authors.affiliation: Division of Risk Assessment, National Institute of Hygienic Sciences, Tokyo, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Aida
authors.forename: K
authors.initials: K
authors.lastname: Takada
authors.forename: O
authors.initials: O
authors.lastname: Uchida
authors.forename: K
authors.initials: K
authors.lastname: Yasuhara
authors.forename: Y
authors.initials: Y
authors.lastname: Kurokawa
authors.forename: M
authors.initials: M
authors.lastname: Tobe
mesh_terms: D000284:Administration, Oral; D000821:Animal Feed; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D004339:Drug Compounding; D005260:Female; D006842:Hydrocarbons, Brominated / Q000008:administration & dosage / Q000633:toxicity*; D006846:Hydrocarbons, Halogenated / Q000008:administration & dosage / Q000633:toxicity*; D008099:Liver / Q000187:drug effects / Q000473:pathology; D008297:Male; D009336:Necrosis; D051381:Rats; D017208:Rats, Wistar; D022882:Trihalomethanes
chemical_list: D006842:Hydrocarbons, Brominated; D006846:Hydrocarbons, Halogenated; D022882:Trihalomethanes; C032707:chlorodibromomethane; C025191:bromodichloromethane; C015044:bromoform