[The effect of hyperthermia against pancreatic cancer cell--various examinations including flow cytometric bromodeoxyuridine/DNA analysis].
We investigated the effect of hyperthermia against pancreatic cancer cell in various aspects including of cytokinetics. The hyperthermia above 43 degrees C was thought to have strong effect for the studies on surviving cell number and morphological change of Capan-2 and RWP-1 cells after heating treatment. The cell inoculated to the nude mice subcutaneously after heating treatment at 43 degrees C was recognized the tumor forming at 3 weeks later, however, the growth speed was slower than that of control to which untreated Capan-2 cells were inoculated. As the case of 44 degrees C, the tumor forming was not recognized at all. The cytokinetics was measured by flow cytometric bromodeoxyuridine (BrdUrd)/DNA analysis. Up to 43 degrees C in accordance with increase in the heating temperature, the accumulation in G2M phase increased, and the amount of BrdUrd incorporated 5 phase decreased. At the temperature above 44 degrees C, BrdUrd was hardly incorporated into S phase cells and the cell cycle was almost stopped. These results suggested the usefulness of hyperthermia and strong effect of heating treatment above 44 degrees C against pancreatic cancer cell.
Nakata
B
B
First Department of Surgery, Osaka City University Medical School, Japan.
Chung
Y S
YS
Sawada
T
T
Yokomatsu
H
H
Tanaka
H
H
Satake
K
K
Sowa
M
M
D000818:Animals; D001973:Bromodeoxyuridine; D002453:Cell Cycle; D004273:DNA, Neoplasm / Q000032:analysis*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D006979:Hyperthermia, Induced; D051379:Mice; D008819:Mice, Nude; D010190:Pancreatic Neoplasms / Q000737:chemistry / Q000473:pathology*; D014407:Tumor Cells, Cultured / Q000737:chemistry / Q000473:pathology
D004273:DNA, Neoplasm; D001973:Bromodeoxyuridine