[Studies on metabolism of a fungicide yekuling in rat].
The metabolism of a fungicide Yekuling was studied in rat after oral administration. Four metabolites were isolated and purified by means of reverse-phase HPLC and TLC. They were identified to be acetic acid, 1,3-dithiolan-2-ylidenehydrazide; pyruvic acid, 1,3-dithiolan-2-ylidenehydrazide; benzoic acid and hippuric acid with UV and MS. The latter was further confirmed by chemical synthesis. The experimental results showed that Yekuling was metabolized extensively in rat. Yekuling was hydrolyzed by amidase to produce benzoic acid and 1,3-dithiolan-2-ylidenehydrazide. The latter was further acetylated by N-acetyltransferase to form acetic acid, 1,3-dithiolan-2-ylidenehydrazide, or condensed spontaneously to form pyruvic acid, 1,3-dithiolan-2-ylidenehydrazide. Benzoic acid was further conjugated with glycine to produce hippuric acid.
Gao
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Department Environmental Health, Third Military Medical College, Chongqing.
D000085:Acetates / Q000378:metabolism; D019342:Acetic Acid; D000818:Animals; D001565:Benzoates / Q000378:metabolism; D019817:Benzoic Acid; D005659:Fungicides, Industrial / Q000378:metabolism*; D006835:Hydrazones / Q000378:metabolism*; D008297:Male; D011773:Pyruvates / Q000378:metabolism; D019289:Pyruvic Acid; D051381:Rats; D011919:Rats, Inbred Strains
D000085:Acetates; D001565:Benzoates; D005659:Fungicides, Industrial; D006835:Hydrazones; D011773:Pyruvates; C078502:2-acetoylhydrazono-1,3-dithiolane; C078503:2-pyruvoylhydrazono-1,3-dithiolane; C049780:2-benzoylhydrazono-1,3-dithiolane; D019289:Pyruvic Acid; D019817:Benzoic Acid; D019342:Acetic Acid