DNA conformational change induced by the bacteriophage phi 29 connector.
Translocation of viral DNA inwards and outwards of the capsid of double-stranded DNA bacteriophages occurs through the connector, a key viral structure that is known to interact with DNA. It is shown here that phage phi 29 connector binds both linear and circular double-stranded DNA. However, DNA-mediated protection of phi 29 connectors against Staphylococcus aureus endoprotease V8 digestion suggests that binding to linear DNA is more stable than to circular DNA. Endoprotease V8-protection assays also suggest that the length of the linear DNA required to produce a stable phi 29 connector-DNA interaction is, at least, twice longer than the phi 29 connector channel. This result is confirmed by experiments of phi 29 connector-protection of DNA against DNase I digestion. Furthermore, DNA circularization assays indicate that phi 29 connectors restrain negative supercoiling when bound to linear DNA. This DNA conformational change is not observed upon binding to circular DNA and it could reflect the existence of some left-handed DNA coiling or DNA untwisting inside of the phi 29 connector channel.
Valpuesta
J M
JM
Centro de Biología Molecular (CSIC-UAM), Universidad Autónoma de Madrid, Spain.
Serrano
M
M
Donate
L E
LE
Herranz
L
L
Carrascosa
J L
JL
D017103:Bacillus Phages / Q000235:genetics*; D004279:DNA, Viral / Q000737:chemistry* / Q000648:ultrastructure; D004268:DNA-Binding Proteins / Q000502:physiology* / Q000648:ultrastructure; D008854:Microscopy, Electron; D009690:Nucleic Acid Conformation; D014764:Viral Proteins / Q000502:physiology* / Q000648:ultrastructure
D004279:DNA, Viral; D004268:DNA-Binding Proteins; D014764:Viral Proteins