Using two-dimensional gel electrophoresis to study immune response against intracellular bacterial infection.
Principal component analysis was applied to two-dimensional (2-D) gel electrophoresis patterns, obtained in various phases of infection. Untreated controls could be satisfactorily differentiated from patterns after infection on days 3 and 7 whereas day 10 of infection was grouped with the controls. Comparison of host cellular protein patterns could help to classify in vivo developing infection without requiring any so-called immune marker functions. Immunoaffinity separation of infected cells treated with detergent, followed by 2-D electrophoresis of negative as well as positive eluates, did not reveal radiolabeled bacterial protein antigens.
Kovarova
H
H
Faculty of Medicine, Charles University, Hradec Kralove, Czechoslovakia.
Stulik
J
J
Macela
A
A
Lefkovits
I
I
Skrabkova
Z
Z
D000818:Animals; D000942:Antigens, Bacterial / Q000096:biosynthesis / Q000302:isolation & purification; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000302:isolation & purification; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D005604:Francisella tularensis / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D008809:Mice, Inbred C3H; D011506:Proteins / Q000302:isolation & purification; D013997:Time Factors; D014406:Tularemia / Q000276:immunology* / Q000382:microbiology
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D011506:Proteins