Isologous monoclonal antibodies can induce anti-GBM glomerulonephritis in rats.
Injection of isologous monoclonal antibodies (SR2, SR3) caused anti-glomerular basement membrane antibody-induced glomerulonephritis (anti-GBM nephritis) in WKY/NCrj rats. The antibodies were obtained from hybridoma cells derived from fusion of the spleen of a nephritic WKY/NCrj rat injected with rat solubilized renal basement membranes with adjuvant, and mouse SP2-myeloma cells. They belonged to the rat IgG2a subclass and bound to rat kidney in a linear pattern along the glomerular and tubular basement membranes. Histological changes in glomeruli were detected at day 1 after the injection; proteinuria with haematuria appeared on day 2; and proteinuria became severe and reached a plateau by day 5. These results demonstrate that anti-GBM nephritis can even be induced by an isologous monoclonal antibody and that the rat IgG2a subclass is at least nephritogenic. The experimental model of anti-GBM nephritis with isologous monoclonal antibodies makes it possible and easier to analyse further the mechanism of anti-GBM nephritis.
Sado
Y
Y
Division of Immunology, Shigei Medical Research Institute, Okayama, Japan.
Kagawa
M
M
Rauf
S
S
Naito
I
I
Moritoh
C
C
Okigaki
T
T
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D001485:Basement Membrane / Q000276:immunology; D004195:Disease Models, Animal; D004306:Dose-Response Relationship, Immunologic; D005260:Female; D005455:Fluorescent Antibody Technique; D015433:Glomerulonephritis, Membranous / Q000276:immunology* / Q000473:pathology; D007074:Immunoglobulin G / Q000276:immunology; D007678:Kidney Glomerulus / Q000276:immunology* / Q000473:pathology; D051381:Rats; D011921:Rats, Inbred WKY
D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G