title: Mutational analysis of a dominant oncogene (c-Ki-ras-2) and a tumor suppressor gene (p53) in hamster lung tumorigenesis.
abstract: In human lung cancers, alterations of both a dominant oncogene (ras) and a tumor suppressor gene (p53) have been identified. Polymerase chain reaction (PCR) analysis of mRNA was used to amplify the c-Ki-ras-2 and p53 genes from Syrian golden hamsters. The PCR products were confirmed by predicted-size analysis, probing with nonradioactive (biotin-labeled) oligonucleotides, and direct sequencing. Lung tumors were produced in hamsters by repeated injections of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK). Of six tumors examined, three (50%) had mutations in codon 12 of Ki-ras. Examination of the conserved regions of p53 revealed no mutations. We conclude that NNK-induced carcinogenesis in the hamster results in characteristic alterations of Ki-ras but may not necessarily involve the p53 gene.
authors.affiliation: Toxic Substances Research and Teaching Program, University of California, Davis 95616-8615.
authors.forename: V I
authors.initials: VI
authors.lastname: Oreffo
authors.forename: H W
authors.initials: HW
authors.lastname: Lin
authors.forename: P H
authors.initials: PH
authors.lastname: Gumerlock
authors.forename: S A
authors.initials: SA
authors.lastname: Kraegel
authors.forename: H
authors.initials: H
authors.lastname: Witschi
mesh_terms: D000818:Animals; D001483:Base Sequence; D006224:Cricetinae; D004252:DNA Mutational Analysis; D016158:Genes, p53 / Q000235:genetics*; D011905:Genes, ras / Q000235:genetics*; D008175:Lung Neoplasms / Q000235:genetics*; D008297:Male; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000737:chemistry
chemical_list: D012333:RNA, Messenger