title: Urinary amphetamines, benzodiazepines and methadone: cost-effective detection procedures.
abstract: Cost-effective immunoassays for the detection of amphetamines, benzodiazepines, and methadone in urine have been developed using Syva EMIT reagents and a Cobas Bio centrifugal analyser. With this method up to 2470 samples can be assayed with a single 100 test EMIT kit while maintaining acceptable precision. Mean CVs of 3.8-6.6% were obtained for concentrations around the manufacturer's recommended threshold level (300 micrograms/l). Comparison of the methods with the Abbott TDx system showed good correlation for methadone. The methods compared less well for amphetamines, and it was not possible to obtain a useful correlation for benzodiazepines. Heat treatment prior to analysis did not affect the detection of benzodiazepines and methadone; there was a mean decrease of 14% for amphetamines.
authors.affiliation: Dept. of Clinical Chemistry, Royal Infirmary, Edinburgh, Scotland, UK.
authors.forename: F M
authors.initials: FM
authors.lastname: Moore
authors.forename: D R
authors.initials: DR
authors.lastname: Jarvie
authors.forename: D
authors.initials: D
authors.lastname: Simpson
mesh_terms: D000662:Amphetamines / Q000652:urine; D001569:Benzodiazepines / Q000652:urine; D003362:Cost-Benefit Analysis; D006801:Humans; D007118:Immunoassay / Q000191:economics*; D008691:Methadone / Q000652:urine; D015813:Substance Abuse Detection / Q000191:economics*
chemical_list: D000662:Amphetamines; D001569:Benzodiazepines; D008691:Methadone