Amyloid precursor protein accumulates in regions of neurodegeneration following focal cerebral ischemia in the rat.
The distribution of beta-amyloid precursor protein (APP) was examined immunocytochemically in rats subjected to focal cerebral ischemia by permanent occlusion of the middle cerebral artery. At 4 and 7 days post-occlusion, APP immunoreactivity was preferentially localized within axonal swellings, dystrophic neurites and neuronal perikarya all along the periphery of the infarct. Immunolabeling was observed with antibodies generated against N-terminal, midregion, and C-terminal domains of APP. No immunoreactivity was observed with antisera directed against beta-amyloid protein (beta A4) itself. This pathological accumulation of APP is consistent with alterations of APP recently described in other models of neurodegeneration and implies a role for this protein in the response to CNS injury.
Stephenson
D T
DT
Eli Lilly and Co., CNS Division, Indianapolis, IN 46285.
Rash
K
K
Clemens
J A
JA
D016564:Amyloid beta-Protein Precursor / Q000378:metabolism*; D000818:Animals; D001369:Axons / Q000378:metabolism / Q000648:ultrastructure; D001921:Brain / Q000378:metabolism* / Q000473:pathology; D002536:Cerebral Arteries; D002544:Cerebral Infarction / Q000378:metabolism / Q000473:pathology; D007150:Immunohistochemistry; D002546:Ischemic Attack, Transient / Q000378:metabolism* / Q000473:pathology; D008297:Male; D009410:Nerve Degeneration; D016501:Neurites / Q000378:metabolism / Q000648:ultrastructure; D009474:Neurons / Q000378:metabolism / Q000473:pathology; D051381:Rats; D011918:Rats, Inbred SHR
D016564:Amyloid beta-Protein Precursor