title: In vitro antibacterial activity of the lactoperoxidase system towards enterotoxigenic strains of Escherichia coli.
abstract: The lactoperoxidase-thiocyanate-hydrogen peroxide (LP) system inhibited the growth of enterotoxigenic Escherichia coli strains responsible for scouring in neonatal and post-weaning piglets. An enzymatic system for hydrogen peroxide generation (glucose oxidase, GO; 0.1 U/ml) and a chemical source (sodium carbonate peroxyhydrate, SCP; 90 mg/l) were used in the LP system to test 19 strains in a 6-h growth assay at 37 degrees C. Only three strains were highly sensitive to the LP/GO system, while all exhibited significant growth inhibition with the LP/SCP system. Hydrogen peroxide alone had less effect than the complete system. The bactericidal activity of the LP/GO system towards a previously resistant strain was greatly increased by increasing the level of glucose oxidase in the system by three- or five-fold.
authors.affiliation: International Food Institute of Queensland, Australia.
authors.forename: P A
authors.initials: PA
authors.lastname: Grieve
authors.forename: D A
authors.initials: DA
authors.lastname: Dionysius
authors.forename: A C
authors.initials: AC
authors.lastname: Vos
mesh_terms: D000818:Animals; D003967:Diarrhea / Q000382:microbiology / Q000662:veterinary*; D004768:Enterotoxins / Q000096:biosynthesis; D004926:Escherichia coli / Q000187:drug effects*; D004927:Escherichia coli Infections / Q000382:microbiology / Q000662:veterinary*; D007784:Lactoperoxidase / Q000494:pharmacology*; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology*
chemical_list: D004768:Enterotoxins; D007784:Lactoperoxidase