Immobilization of DNA for scanning probe microscopy.
Reproducible scanning tunneling microscope and atomic force microscope images of entire molecules of uncoated plasmid DNA chemically bound to surfaces are presented. The chemically mediated immobilization of DNA to surfaces and subsequent scanning tunneling microscope imaging of DNA molecules demonstrate that the problem of molecular instability to forces exerted by the probe tip, inherent with scanning probe microscopes, can be prevented.
Allison
D P
DP
Health and Safety Research Division, Oak Ridge National Laboratory, TN 37831-6123.
Bottomley
L A
LA
Thundat
T
T
Brown
G M
GM
Woychik
R P
RP
Schrick
J J
JJ
Jacobson
K B
KB
Warmack
R J
RJ
D004269:DNA, Bacterial / Q000648:ultrastructure*; D004270:DNA, Circular / Q000648:ultrastructure*; D004355:Drug Stability; D004926:Escherichia coli; D007202:Indicators and Reagents; D008853:Microscopy / Q000379:methods; D016252:Microscopy, Scanning Tunneling / Q000379:methods; D010761:Phosphorus Radioisotopes; D010957:Plasmids
D004269:DNA, Bacterial; D004270:DNA, Circular; D007202:Indicators and Reagents; D010761:Phosphorus Radioisotopes