[Synthetic bioantioxidants--inhibitors of acetylcholinesterase activity].
The inhibitory effects of synthetic antioxidants (3-oxypyridine, pyrimidine and hindered phenols) on the enzymic activity of membrane-bound acetylcholinesterase (AChE) was studied. In terms of estimated kinetic characteristics of AChE-reaction (KM, Vmax, KI), the pattern of enzyme inhibition by the hindered phenol compounds was found to be of non-competitive or mixed type depending on the inhibitor structure or on the substrate acetylcholine or acetylthiocholine used. The comparative study of the inhibitory action of water-soluble derivatives of hindered phenols and fatty-soluble ionol made it possible to reveal possible contributions to the inhibition of both direct and mediated (by the membrane microsurroundings) effects on the membrane-bound AChE by the studied synthetic bioantioxidants.
Braginskaia
F I
FI
Zorina
O M
OM
Molochkina
E M
EM
Nikiforov
G A
GA
Burlakova
E B
EB
D000109:Acetylcholine / Q000097:blood; D000110:Acetylcholinesterase / Q000097:blood / Q000187:drug effects; D000975:Antioxidants / Q000494:pharmacology*; D002800:Cholinesterase Inhibitors / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000201:enzymology; D006801:Humans; D006868:Hydrolysis; D012995:Solubility; D013379:Substrate Specificity / Q000187:drug effects
D000975:Antioxidants; D002800:Cholinesterase Inhibitors; D000110:Acetylcholinesterase; D000109:Acetylcholine
Sinteticheskie bioantioksidanty--ingibitory atsetilkholinésteraznoĭ aktivnosti.