Analysis of legume oligosaccharides by high-resolution gas chromatography.
The suitability of high-resolution gas chromatography (HGRC) for the analysis of the raffinose family oligosaccharides (raffinose, stachyose, verbascose) was investigated. Aqueous methanol (80%) extracts of pea flour were dried and derivatized with either trimethylimidazole or N-methyl-bis(trifluoroacetamide). Separation of the sugar derivatives was achieved utilizing a 10-m DB5-60W capillary column. The effects of carrier gas (He) flow-rate and split ratio on resolution and reproducibility were studied. HRGC analysis was characterized by excellent resolution and satisfactory reproducibility, and proved to be a rapid, sensitive method for quantitation of oligosaccharides in pea flours.
Karoutis
A I
AI
Department of Applied Microbiology and Food Science, University of Saskatchewan, Saskatoon, Canada.
Tyler
R T
RT
Slater
G P
GP
D002849:Chromatography, Gas / Q000592:standards; D007887:Fabaceae / Q000737:chemistry*; D005433:Flour / Q000032:analysis; D007202:Indicators and Reagents; D009844:Oligosaccharides / Q000032:analysis* / Q000592:standards; D010946:Plants, Medicinal; D014269:Trifluoroacetic Acid; D014297:Trimethylsilyl Compounds
D007202:Indicators and Reagents; D009844:Oligosaccharides; D014297:Trimethylsilyl Compounds; D014269:Trifluoroacetic Acid