Evidence for increased calcium influx across the choroid plexus following brief ischemia of gerbil brain.
Following 5 min ischemia of gerbil brain, unidirectional transfer of calcium from plasma to cerebrospinal fluid was measured quantitatively with 45CaCl2 at 4 days postischemic recirculation. 45Ca influx across the choroid plexus increased significantly from 0.0101 +/- 0.001 min-1 measured in sham-operated animals (n = 15) to 0.0294 +/- 0.002 min-1 determined in ischemic animals (n = 21; P less than 0.05). Histological examination of choroid plexus was carried out in Cresyl violet-stained sections from gerbils subjected to 5 min ischemia followed by 4 days (n = 6) and 7 days (n = 7) postischemic recirculation. Increased calcium transfer to cerebrospinal fluid was associated with cell damage of choroid plexus observed at 4 days postischemia. Endothelial choroid plexus injury was still detectable at 7 days after the ischemic insult suggesting a long-lasting pathomorphological process. Postischemic alterations in choroid plexus functions apparently expose brain tissue to much higher calcium influx into cerebrospinal fluid which, in turn, may contribute to calcium-related cell damage of the central nervous system.
Ikeda
J
J
Laboratory of Neuropathology and Neuroanatomical Sciences, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892.
Mies
G
G
Nowak
T S
TS
Joó
F
F
Klatzo
I
I
D000818:Animals; D002118:Calcium / Q000134:cerebrospinal fluid / Q000378:metabolism*; D002132:Calcium Radioisotopes; D002831:Choroid Plexus / Q000378:metabolism*; D005260:Female; D005849:Gerbillinae; D002546:Ischemic Attack, Transient / Q000378:metabolism*
D002132:Calcium Radioisotopes; D002118:Calcium