An inducible expression system for the production of human lactoferrin in Aspergillus nidulans.
The production and secretion of human lactoferrin (hLF) in Aspergillus nidulans is described. The hLF cDNA was expressed under the control of the strong ethanol-inducible alcohol dehydrogenase (alcA) promoter. Recombinant hLF (re-hLF) is produced at levels up to 5 micrograms/ml. Approximately 30% of the re-hLF produced in this system is secreted into the growth medium. The re-hLF is indistinguishable from native hLF with respect to size and immunoreactivity. Furthermore, re-hLF is functional by the criterion of iron-binding capacity. The A. nidulans expression system offers an inexpensive, convenient method for the controlled production of mg amounts of biologically active mammalian glycoproteins.
Ward
P P
PP
Department of Cell Biology, Baylor College of Medicine, Houston, TX 77030.
May
G S
GS
Headon
D R
DR
Conneely
O M
OM
D001233:Aspergillus nidulans; D015139:Blotting, Southern; D015153:Blotting, Western; D003001:Cloning, Molecular / Q000379:methods; D004247:DNA; D006801:Humans; D007501:Iron / Q000378:metabolism; D007781:Lactoferrin / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D010957:Plasmids; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism
D011994:Recombinant Proteins; D004247:DNA; D007501:Iron; D007781:Lactoferrin