[Plasmids for biodegradation of 2,6-dimethylpyridine, 2,4-dimethylpyridine, and pyridine in strains of Arthrobacter].
Arthrobacter crysallopoietes strain KM-4 degrading 2,6-dimethylpyridine and strain KM-4a degrading both 2,6-dimethylpyridine and pyridine, Arthrobacter sp. KM-4b degrading 2,4-dimethylpyridine were isolated from soil. Arthrobacter crystallopoietes KM-4 and Arthrobacter sp. KM-4b contain 100 Md plasmids pBS320 and pBS323. Arthrobacter crystallopietes KM-4a harbours a 100 Md and 80 Md plasmids. Plasmid curing and conjugation transfer results confirm that these plasmids are involved in degradation of 2,6-dimethylpyridine, 2,4-dimethylpyridine and pyridine. A mutant with lost ability to degrade 2,6-dimethylpyridine was isolated during the growth of strain KM-4 rifR at 42 degrees C. Electrophoretic analysis of the plasmid from temperature sensitive mutant revealed the deletion the size of 26 Md from pBS320 plasmid.
Agapova
S R
SR
Andreeva
A L
AL
Starovoĭtov
I I
II
Vorob'eva
L I
LI
Terent'ev
P B
PB
D001173:Arthrobacter / Q000235:genetics* / Q000378:metabolism; D001673:Biodegradation, Environmental; D004269:DNA, Bacterial / Q000378:metabolism; D005798:Genes, Bacterial; D010957:Plasmids; D011725:Pyridines / Q000493:pharmacokinetics*
D004269:DNA, Bacterial; D011725:Pyridines; C013093:2,6-lutidine; C078448:2,4-dimethylpyridine; C023666:pyridine
Plazmidy biodegradatsii 2,6-dimetilpiridina, 2,4-dimetilpiridina i piridina u shtammov Artrobacter.