title: Transfection of mouse cytotoxic T lymphocyte with an antisense granzyme A vector reduces lytic activity.
abstract: Murine CTL have seven serine proteases, known as granzymes, in their lytic granules. Despite considerable effort, convincing evidence that these enzymes play an obligatory role in the lytic process has not been presented. To investigate the function of one of these proteases, granzyme A (GA), we utilized an antisense expression vector to lower the level of the enzyme in the cells. An expression vector containing antisense cDNA for GA and the gene for hygromycin B resistance was constructed and electroporated into the murine CTL line, AR1. Transfectants were selected based on resistance to hygromycin B, and a number of stable lines were developed. One of the antisense lines had greatly reduced levels of GA mRNA, when compared to the parental cells or to control lines transfected with the vector lacking the antisense DNA. The message levels for two other CTL granule proteins, granzyme B and perforin, were unaffected by the antisense vector. The amount of GA, as measured by enzymatic activity, was 3- to 10-fold lower in the transfectant. Most significantly, this line also consistently showed 50 to 70% lower ability to lyse nucleated target cells and to degrade their DNA. Furthermore, it exhibited 90 to 95% lower lytic activity to anti-CD3-coated SRBC. Conjugate formation with target cells, however, was normal. These data provide strong evidence that GA plays an important role in the cytolytic cycle, and that the quantity of enzyme is a limiting factor in these cytolytic cells.
authors.affiliation: Merck Research Laboratories, Rahway, NJ 07065.
authors.forename: A
authors.initials: A
authors.lastname: Talento
authors.forename: M
authors.initials: M
authors.lastname: Nguyen
authors.forename: S
authors.initials: S
authors.lastname: Law
authors.forename: J K
authors.initials: JK
authors.lastname: Wu
authors.forename: M
authors.initials: M
authors.lastname: Poe
authors.forename: J T
authors.initials: JT
authors.lastname: Blake
authors.forename: M
authors.initials: M
authors.lastname: Patel
authors.forename: T J
authors.initials: TJ
authors.lastname: Wu
authors.forename: C L
authors.initials: CL
authors.lastname: Manyak
authors.forename: M
authors.initials: M
authors.lastname: Silberklang
mesh_terms: D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002460:Cell Line; D003601:Cytotoxicity Tests, Immunologic; D003602:Cytotoxicity, Immunologic / Q000235:genetics*; D016373:DNA, Antisense / Q000494:pharmacology*; D004351:Drug Resistance; D005822:Genetic Vectors; D053804:Granzymes; D006921:Hygromycin B / Q000276:immunology; D007770:L-Lactate Dehydrogenase / Q000096:biosynthesis; D008562:Membrane Glycoproteins / Q000096:biosynthesis; D051379:Mice; D008969:Molecular Sequence Data; D054353:Perforin; D016133:Polymerase Chain Reaction; D052899:Pore Forming Cytotoxic Proteins; D016372:RNA, Antisense / Q000096:biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis; D012697:Serine Endopeptidases / Q000096:biosynthesis / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014162:Transfection
chemical_list: D016373:DNA, Antisense; D008562:Membrane Glycoproteins; D052899:Pore Forming Cytotoxic Proteins; D016372:RNA, Antisense; D012333:RNA, Messenger; D054353:Perforin; D006921:Hygromycin B; D007770:L-Lactate Dehydrogenase; D053804:Granzymes; C509214:Gzmb protein, mouse; D012697:Serine Endopeptidases