Correlation between P19 presence and MHC class II expression in human fetal astroglial cells cocultured with HTLV-I donor cells.
The possibility of a direct infection of human brain by HTLV-I, has been studied using an in vitro model. Human fetal astroglial cells were cocultivated with irradiated HTLV-I donor cell line MT-2, and assayed for the presence of HTLV-I core protein p19 after 1 week. Fifty-six per cent of GFAP positive astrocytes showed the viral core protein p19 and increased expression of Class II MHC antigens. Electron microscopy of astroglial cells exposed to HTLV-I revealed the presence of vacuoli-like structures containing viral core protein p19. Cell intermediate filament cytoskeleton was also disorganized. Even if this study does not provide direct evidence for virus replication inside astroglial cells, all these findings suggest that HTLV-I can indeed enter the cell and exert a cytopathic effect. Therefore the results of the present study are consistent with the hypothesis that astroglial cells could be involved in demyelination processes occurring in the HTLV-I associated neurological disorders, such as human associated myelopathy and tropical spastic paraparesis.
Macchi
B
B
Department of Experimental Medicine and Biochemical Sciences, II University of Rome, Tor Vergata, Italy.
Caronti
B
B
Cocchia
D
D
Gremo
F
F
Torelli
S
S
Sogos
V
V
Bonmassar
E
E
Lauro
G M
GM
D001253:Astrocytes / Q000276:immunology / Q000382:microbiology / Q000502:physiology*; D001921:Brain / Q000196:embryology* / Q000276:immunology; D002461:Cell Line, Transformed; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000196:embryology / Q000276:immunology; D005314:Embryonic and Fetal Development; D005333:Fetus; D015683:Gene Products, gag / Q000032:analysis*; D005865:Gestational Age; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D006681:HLA-D Antigens / Q000096:biosynthesis* / Q000235:genetics; D015368:Human T-lymphotropic virus 1 / Q000276:immunology / Q000502:physiology*; D006801:Humans; D015682:Retroviridae Proteins, Oncogenic / Q000032:analysis*; D014758:Viral Core Proteins / Q000032:analysis*; D054301:gag Gene Products, Human Immunodeficiency Virus
D015683:Gene Products, gag; D005904:Glial Fibrillary Acidic Protein; D006681:HLA-D Antigens; D015682:Retroviridae Proteins, Oncogenic; D014758:Viral Core Proteins; D054301:gag Gene Products, Human Immunodeficiency Virus; C060955:p19 protein, Human T-lymphotropic virus 1