Initiation on chromatin templates in a yeast RNA polymerase II transcription system.
Templates were prepared with either the TATA box or transcription start sites of the yeast CYC1 promoter in a nucleosome. In both cases, initiation in an unfractionated yeast RNA polymerase II transcription system was abolished by the nucleosome. The inhibition appeared to be relieved by the activator protein Gal4-VP16 binding to a site upstream of the promoter. Inhibition was not relieved, however, in a transcription system reconstituted from purified components, indicating a requirement for additional factors for the effect of Gal4-VP16.
Lorch
Y
Y
Department of Cell Biology, Stanford University School of Medicine, California 94305.
LaPointe
J W
JW
Kornberg
R D
RD
D001483:Base Sequence; D002843:Chromatin / Q000378:metabolism*; D004271:DNA, Fungal; D005656:Fungal Proteins / Q000378:metabolism; D008969:Molecular Sequence Data; D009707:Nucleosomes / Q000378:metabolism; D011401:Promoter Regions, Genetic; D012319:RNA Polymerase II / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics; D016385:TATA Box; D013698:Templates, Genetic; D015534:Trans-Activators / Q000378:metabolism; D014158:Transcription, Genetic
D002843:Chromatin; D004271:DNA, Fungal; D005656:Fungal Proteins; C070633:Gal-VP16; D009707:Nucleosomes; D015534:Trans-Activators; D012319:RNA Polymerase II