An 87-kilodalton glucan-binding protein of Streptococcus sobrinus B13.
An 87-kDa glucan-binding protein (GBP) of Streptococcus sobrinus B13 (serotype d) was isolated and purified from extracellular culture supernatant by using affinity chromatography on Sephadex G-50 and elution with a guanidine HCl gradient. Western blot (immunoblot) analysis showed it to be antigenically related, but not completely identical, to the 74-kDa GBP of Streptococcus mutans Ingbritt. The 87-kDa GBP has no glucosyltransferase activity. A possible role for this GBP in the cariogenicity of S. sobrinus B13 is suggested.
Wu-Yuan
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Dows Institute for Dental Research, University of Iowa College of Dentistry, Iowa City 52242.
Gill
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D002352:Carrier Proteins / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification*; D005936:Glucans / Q000378:metabolism*; D005964:Glucosyltransferases / Q000032:analysis; D037102:Lectins; D016986:Streptococcus sobrinus / Q000737:chemistry*
D002352:Carrier Proteins; D005936:Glucans; D037102:Lectins; C037937:glucan-binding proteins; D005964:Glucosyltransferases