Decrease in immunoreactivity and vasoactivity of endothelin-1 after exposure to oxygen.
Endothelin-1 (ET-1) caused a dose-dependent contraction of porcine coronary arterial rings. Preincubation of ET-1 in oxygenated Krebs solution at 37 degrees C resulted in a progressive loss of the contractile activity and immunoreactivity of ET-1. The half-life of ET-1 contractile activity in oxygenated and non-oxygenated buffer was 16.1 and 86.6 min., respectively. The molecular weight of ET-1 exposed to oxygen was identical to that of ET-1. These results indicate that during the contraction of smooth muscle preparations the levels of ET-1 in the tissue bath are steadily decreasing due to exposure to high pO2 levels.
Balwierczak
J L
JL
Research Dept., Ciba-Geigy Corp., Summit, N.J. 07901.
Hopkins
M F
MF
Savage
P
P
Martin
L L
LL
Jeng
A Y
AY
D000818:Animals; D016232:Endothelins / Q000494:pharmacology*; D066298:In Vitro Techniques; D008970:Molecular Weight; D009119:Muscle Contraction / Q000187:drug effects; D009131:Muscle, Smooth, Vascular / Q000187:drug effects*; D010100:Oxygen / Q000494:pharmacology*; D011863:Radioimmunoassay; D016339:Spectrometry, Mass, Fast Atom Bombardment; D013552:Swine
D016232:Endothelins; D010100:Oxygen