Simultaneous determination of some antibacterial drugs in isosensitest broth using high-performance liquid chromatography with solid-phase extraction.
A method is described for the simultaneous determination of combinations of some antibacterial drugs in a matrix of isosensitest broth. A double solid-phase extraction procedure is described in which trimethoprim and dibromopropamidine isethionate together with 4-chlorophenylbiguanide as internal standard are freed from endogenous components by a cation-exchange extraction cartridge and subsequently removed and individually separated by reversed-phase ion-pair chromatography. Sulfadiazine, sulfamerazine and p-aminobenzoic acid, unretained by ion exchange, are similarly isolated for chromatography by adsorption on a CH-bonded phase cartridge and individually assayed using the same chromatographic system. The rationale of the pre-treatment and chromatography is described and the quantitative aspects of the analyses of selected combinations of these drugs are reported.
Taylor
R B
RB
School of Pharmacy, Robert Gordon University, Schoolhill, Aberdeen, UK.
Richards
M E
ME
Xing
J Z
JZ
D010129:4-Aminobenzoic Acid / Q000032:analysis; D000890:Anti-Infective Agents / Q000032:analysis*; D002623:Chemistry Techniques, Analytical / Q000379:methods; D002851:Chromatography, High Pressure Liquid; D003470:Culture Media / Q000737:chemistry*; D013411:Sulfadiazine / Q000032:analysis; D013416:Sulfamerazine / Q000032:analysis; D014295:Trimethoprim / Q000032:analysis
D000890:Anti-Infective Agents; D003470:Culture Media; D013411:Sulfadiazine; D014295:Trimethoprim; D010129:4-Aminobenzoic Acid; D013416:Sulfamerazine