title: Developmental changes in testosterone production by the rat testis in vitro during late fetal life.
abstract: The age-related evolution of the in vitro capacity of the rat testis to produce testosterone and to respond to luteinizing hormone (LH) during 3 and 24 h incubation was studied from day 18.5 to day 21.5 of fetal life. Basal testosterone production by testes from 18.5-day-old fetuses was significantly higher than production by testes from 20.5- and 21.5-day-old fetuses when secretion was expressed as either per testis or per microgram of testicular protein. When maximal LH-stimulated testosterone secretion was expressed on a per testis basis, it was significantly lower for day 18.5 testes than for day 20.5 and 21.5 testes. However, when it was expressed on the basis of testicular protein content, it decreased significantly between days 18.5 and 20.5. Basal and LH-stimulated secretions displayed the same time-related decrease throughout 24 h of incubation for the three ages studied. The dose-response curves for LH showed that the sensitivity was similar for day 18.5 and 20.5 testes (ED50 = 10 vs. 14 ng/mL, respectively). These results showed an age-related decrease in testicular steroidogenic capacity without a change in the coupling efficiency of LH receptor to testosterone production during late fetal life in rats.
authors.affiliation: Unité de Recherche sur la Différenciation et la Croissance Cellulaire INSERUM U 307, Université Paris.
authors.forename: R
authors.initials: R
authors.lastname: Habert
authors.forename: V
authors.initials: V
authors.lastname: Rouiller-Fabre
authors.forename: L
authors.initials: L
authors.lastname: Lecerf
authors.forename: C
authors.initials: C
authors.lastname: Levacher
authors.forename: J M
authors.initials: JM
authors.lastname: Saez
mesh_terms: D000818:Animals; D046508:Culture Techniques; D004305:Dose-Response Relationship, Drug; D005260:Female; D005865:Gestational Age; D007986:Luteinizing Hormone / Q000008:administration & dosage / Q000494:pharmacology; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013737:Testis / Q000187:drug effects / Q000196:embryology* / Q000378:metabolism*; D013739:Testosterone / Q000096:biosynthesis*; D013997:Time Factors
chemical_list: D013739:Testosterone; D007986:Luteinizing Hormone