[The effect of 3-aminobenzamide on the mitotic cycle of Chinese hamster cells cultured on a medium with 5-bromodeoxyuridine following ionizing radiation action].
A specific inhibitor of poly(ADP-ribose)polymerase-3-aminobenzamide (6 mM) has been shown to: 1) reduce survival of non-irradiated CHO-K1 cells, cultivated in medium containing 5-bromodeoxyuridine (10 mkM, BDU cells), and increase their radiosensitivity; 2) induce G2 delay in BDU cells while progressing through the cell cycle as analysed by the DNA flow cytometry; 3) increase to a great degree G2 delay in X-irradiated BDU cells. 3-Aminobenzamide is primarily effective when it is present during the first or two first cell cycles after the initial addition of BDU. The above data confirm the involvement, presumably an indirect one, of ADP-ribosylation in the DNA repair through affecting the chromatin structure.
Kirillova
T V
TV
Rozanov
Iu M
IuM
Spivak
I M
IM
D000818:Animals; D001549:Benzamides / Q000494:pharmacology*; D001973:Bromodeoxyuridine / Q000494:pharmacology*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D002478:Cells, Cultured / Q000166:cytology / Q000187:drug effects / Q000528:radiation effects; D006224:Cricetinae; D003470:Culture Media; D004347:Drug Interactions; D007399:Interphase / Q000187:drug effects / Q000528:radiation effects; D008938:Mitosis / Q000187:drug effects* / Q000528:radiation effects*; D011838:Radiation-Sensitizing Agents / Q000494:pharmacology*; D016196:S Phase / Q000187:drug effects / Q000528:radiation effects
D001549:Benzamides; D003470:Culture Media; D011838:Radiation-Sensitizing Agents; C025160:3-aminobenzamide; D001973:Bromodeoxyuridine
Vliianie 3-aminobenzamida na mitoticheskiĭ tsikl kletok kitaĭskogo khomiachka, kul'tiviruiumeykh na srede s 5-bromdezoksiuridinom, posle deĭstviia ioniziruiushcheĭ radiatsii.