title: Lipopolysaccharide priming potentiates calcium ionophore stimulated human placental prostaglandin E2 release in vitro.
abstract: In this study, the effect of bacterial endotoxin (lipopolysaccharide; LPS) and of LPS priming on the in vitro release of PGE2 from human placental explants was investigated. Both LPS and the calcium ionophore A23187 significantly stimulated PGE2 release (P less than 0.05). Simultaneous exposure of placental explants to both LPS and A23187 revealed no additive or synergistic stimulation of PGE2 release. LPS priming of placental tissue significantly increased A23187-stimulated PGE2 release when compared to non-LPS-primed tissues. The addition of exogenous arachidonic acid (the substrate for PGE2 synthesis) also significantly (P less than 0.05) stimulated PGE2 release. There was, however, no significant further stimulation of PGE2 release following LPS priming in arachidonic acid treated explants. These data suggest that LPS not only increases basal PGE2 release in human placentae, but also potentiates agonist-stimulated PGE2 release, possibly by increasing tissue capacity for endogenous arachidonic acid liberation.
authors.affiliation: Department of Obstetrics and Gynaecology, Monash University, Clayton, Australia.
authors.forename: W
authors.initials: W
authors.lastname: Gu
authors.forename: G E
authors.initials: GE
authors.lastname: Rice
authors.forename: M
authors.initials: M
authors.lastname: Michael
authors.forename: S P
authors.initials: SP
authors.lastname: Brennecke
mesh_terms: D000001:Calcimycin / Q000494:pharmacology*; D015232:Dinoprostone / Q000378:metabolism*; D004357:Drug Synergism; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D008070:Lipopolysaccharides / Q000494:pharmacology; D010920:Placenta / Q000187:drug effects / Q000378:metabolism*; D011247:Pregnancy
chemical_list: D008070:Lipopolysaccharides; D000001:Calcimycin; D015232:Dinoprostone