title: Purification of native dengue-2 viral proteins and the ability of purified proteins to protect mice.
abstract: Both the envelope structural protein and the non-structural NS1 protein have been purified from the flavivirus dengue-2 by high-pressure liquid chromatography. These purified proteins maintain their reactivity with monoclonal antibodies. When tested in mice, the envelope protein elicited neutralizing antibodies and partially protected the animals against a lethal viral challenge. The mice responded to the non-structural protein by producing antibodies; however, these antibodies were not neutralizing and the mice were not protected.
authors.affiliation: Department of Virus Diseases, Walter Reed Army Institute of Research, Washington, DC.
authors.forename: R
authors.initials: R
authors.lastname: Feighny
authors.forename: J
authors.initials: J
authors.lastname: Burrous
authors.forename: J
authors.initials: J
authors.lastname: McCown
authors.forename: C
authors.initials: C
authors.lastname: Hoke
authors.forename: R
authors.initials: R
authors.lastname: Putnak
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D015153:Blotting, Western; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D003715:Dengue / Q000517:prevention & control*; D003716:Dengue Virus / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D009500:Neutralization Tests; D011863:Radioimmunoassay; D015164:Vaccines, Inactivated / Q000276:immunology; D014759:Viral Envelope Proteins / Q000276:immunology* / Q000302:isolation & purification; D017361:Viral Nonstructural Proteins / Q000276:immunology* / Q000302:isolation & purification; D014765:Viral Vaccines / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D015164:Vaccines, Inactivated; D014759:Viral Envelope Proteins; D017361:Viral Nonstructural Proteins; D014765:Viral Vaccines