title: The binding of plasminogen fragments to cultured human umbilical vein endothelial cells.
abstract: Glu-plasminogen, kringle 1-5, kringle 1-3, and miniplasminogen exhibited strong binding to human umbilical vein endothelial cells (HUVEC). On the other hand, no significant binding was obtained with microplasminogen and kringle 4. Kringle 1-5 and miniplasminogen, which both contained kringle 5, specifically inhibited the binding of plasminogen to HUVEC while kringle 1-3 did not. The results implied plasminogen molecule contained at least two binding sites, with which it interacted HUVEC. The stronger binding site was located in kringle 5 and the weaker one was in kringle 1-3. Kringle 4 and the active site domain exhibited no significant binding to HUVEC. The interaction of plasminogen with HUVEC is mainly through binding site on kringle 5.
authors.affiliation: Department of Biochemistry, Medical College, National Cheng Kung University, Tainan, Taiwan, Republic of China.
authors.forename: H L
authors.initials: HL
authors.lastname: Wu
authors.forename: I S
authors.initials: IS
authors.lastname: Wu
authors.forename: R Y
authors.initials: RY
authors.lastname: Fang
authors.forename: J S
authors.initials: JS
authors.lastname: Hau
authors.forename: D H
authors.initials: DH
authors.lastname: Wu
authors.forename: B I
authors.initials: BI
authors.lastname: Chang
authors.forename: T M
authors.initials: TM
authors.lastname: Lin
authors.forename: G Y
authors.initials: GY
authors.lastname: Shi
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D002478:Cells, Cultured; D004591:Electrophoresis, Polyacrylamide Gel; D004730:Endothelium, Vascular / Q000378:metabolism*; D006801:Humans; D007457:Iodine Radioisotopes; D007700:Kinetics; D008970:Molecular Weight; D010446:Peptide Fragments / Q000302:isolation & purification / Q000378:metabolism*; D010958:Plasminogen / Q000378:metabolism*; D011487:Protein Conformation; D014471:Umbilical Veins
chemical_list: D007457:Iodine Radioisotopes; D010446:Peptide Fragments; D010958:Plasminogen