Morphometric study of the sensory neuron and peripheral nerve changes induced by chronic cisplatin (DDP) administration in rats.
We performed a morphological, morphometric and toxicological study on the spinal ganglia and peripheral nerves of the rat after chronic administration of cisplatin (cis-dichlorodiammineplatinum II; DDP) with two different schedules. Severe damage of the spinal ganglia neurons was demonstrated with predominant involvement of the nucleus and nucleolus associated with a decrease in the cell size. Morphological and morphometric changes also occurred in the sciatic and peroneal nerves with the features of axonopathy. All these changes were more marked in the group of rats which underwent the most intense DDP treatment and the tissue platinum concentrations were also higher in this group. This experimental model is the first available for chronic DDP administration in which concomitant spinal ganglia and peripheral nerve damage has been confirmed pathologically. Our study supports the hypothesis that DDP-induced peripheral nerve fiber degeneration may result from nuclear and nucleolar changes in the sensory ganglion cell perikaryon.
Cavaletti
G
G
Department of Neurology, Monza, Italy.
Tredici
G
G
Marmiroli
P
P
Petruccioli
M G
MG
Barajon
I
I
Fabbrica
D
D
D000818:Animals; D002466:Cell Nucleolus / Q000187:drug effects / Q000648:ultrastructure; D002945:Cisplatin / Q000633:toxicity*; D005260:Female; D005727:Ganglia, Spinal / Q000187:drug effects / Q000648:ultrastructure; D006651:Histocytochemistry; D007091:Image Processing, Computer-Assisted; D009186:Myelin Sheath / Q000187:drug effects / Q000648:ultrastructure; D009410:Nerve Degeneration / Q000187:drug effects; D009475:Neurons, Afferent / Q000187:drug effects* / Q000648:ultrastructure; D010525:Peripheral Nerves / Q000187:drug effects* / Q000648:ultrastructure; D010543:Peroneal Nerve / Q000187:drug effects / Q000648:ultrastructure; D051381:Rats; D017208:Rats, Wistar; D012584:Sciatic Nerve / Q000187:drug effects / Q000648:ultrastructure
D002945:Cisplatin