[Protection of naturally susceptible animals against foot-and-mouth disease with a peptide, synthesized on a lysine matrix].
A peptide VP1-(142-158)-MAP (Multiple antigen peptide system) consisting of two parts: a lysine matrix made up of three levels of lysine residues coupled with each other and amino acid sequence 142-158 of VP1 of FMD virus strain A(22)550--has been synthesized. Guinea-pigs inoculated with 20 mkg of the peptide incorporated with Freund's complete adjuvant were protected against challenge with 500 ID50 of homologous FMD virus. Sheep were immunized with a single inoculation of the peptide in a dose of 1.0 mg. Cattle inoculated twice with 1.5 mg of the peptide with incomplete adjuvant on the basis of synthetic oil developed high virus-specific antibody titres both after the first (5.3-7.6 log2 ND50/0.1 ml) and the second inoculation (10.2-11.0 log2 ND50/0.1 ml). The peptide-immunized animals were resistant to challenge with homologous virulent virus in a dose of 10(4) ID50. The immunogenic and protective capacities of the peptide VP1-(142-158)-MAP were shown to be greater as compared with those of its linear analogue-peptide VP1-(141-160).
Lugovskoĭ
A A
AA
Rybakov
S S
SS
Ivaniushchenkov
V N
VN
Chepurkin
A V
AV
Petrov
V N
VN
Driagalin
N N
NN
Burdov
A N
AN
D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D002850:Chromatography, Gel; D004198:Disease Susceptibility; D005536:Foot-and-Mouth Disease / Q000517:prevention & control*; D006168:Guinea Pigs; D007114:Immunization; D008239:Lysine / Q000737:chemistry*; D008969:Molecular Sequence Data; D010455:Peptides / Q000627:therapeutic use*
D010455:Peptides; D008239:Lysine
Zashchita estestvenno-vospriimchivykh zhivotnykh ot iashchura peptidom, sintezirovannym na lizinovoĭ matritse.