Differentiated reaction of different types of antheridial cells in Chara vulgaris to the changes in light conditions during 24 hours.
Circadian rhythm of activity of 3H-leucine incorporation into antheridial cells of Chara vulgaris, in natural photoperiod was compared with changes in mitotic activity of antheridial filament cells which form spermatozoids. Three types of cells functionally connected with each other i.e. manubria, capitular cells and antheridial filaments indicate high amplitude (80-90%) changes in circadian translational activity and some similarities in their course. The shield cells are characterized by small circadian changes in translational activity in the range of 15-30% and their different rhythm. Manubria, which are the secretive cells indicated the highest dependence of the dynamic of translational activity on the time of day. Their high activity overlaps light phase, low activity--dark phase. The reaction of capitular cells to day/night change is delayed in comparison with the reaction of manubria, and that of antheridial filaments is delayed in comparison with the capitular cells reaction. The assumption was set forth that manubria play the role of oscillators (starttercells) which induce the wave of changes translocating to the other cells functionally and spatialy connected with them. The course of the wave of antheridial filaments mitotic activity suggests that a distinct drop in MI in the morning may be the result of the lack of the factors necessary for initiation of the mitosis, dependent on light-induced high translational activity of antheridial cells.
Kwiatkowska
M
M
Laboratory of Cytophysiology, University of Lódź, Poland.
Połońska
K
K
Gosek
A
A
Malinowski
S
S
D002455:Cell Division / Q000502:physiology; D000460:Chlorophyta / Q000166:cytology* / Q000378:metabolism / Q000502:physiology*; D002940:Circadian Rhythm / Q000502:physiology*; D004247:DNA / Q000378:metabolism; D007930:Leucine / Q000378:metabolism; D008027:Light; D008938:Mitosis / Q000502:physiology; D013997:Time Factors; D014316:Tritium
D014316:Tritium; D004247:DNA; D007930:Leucine