[Determination of esterase activity of human and animal serine proteinases using fluorogenic esters of amino acids as substrates].
Two fluorogenic derivatives of amino acids are proposed as substrates for the purpose of enzymatic assay: N-benzyloxycarbonyl-phenylalanine-4-methyl umbelliferyl ester (substrate-1) and tert-butyloxycarbonyl-alanine-4-methyl-umbelliferyl ester (substrate-II). Chymotrypsin-like (hydrolysis of substrate-1), elastase-like (hydrolysis of substrate-II) esterase activity of bovine pancreatic chymotrypsin, activities of cathepsin G and elastase from human, porcine and rat neutrophils and esterase activity of human, porcine and rat serum were assayed. Differences in the level of chymotrypsin-like and elastase-like activities of human, porcine and rat serum were established. Activities of purified elastase and cathepsin G from human and animal neutrophils were shown to have no significant distinctions.
Kraeva
L N
LN
Kokriakov
V N
VN
Pozdnev
V F
VF
Morozov
V I
VI
Usova
A A
AA
D000818:Animals; D056649:Cathepsin G; D002403:Cathepsins / Q000378:metabolism; D004950:Esterases / Q000032:analysis / Q000378:metabolism*; D006801:Humans; D006868:Hydrolysis; D006923:Hymecromone / Q000031:analogs & derivatives; D009504:Neutrophils / Q000378:metabolism; D010649:Phenylalanine / Q000031:analogs & derivatives; D051381:Rats; D012697:Serine Endopeptidases / Q000737:chemistry / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013552:Swine
C070487:tert-butyloxycarbonyl-1-alanine 4-methylumbelliferyl ester; C067538:N-benzyloxycarbonylphenylalanine methyl ester; D006923:Hymecromone; D010649:Phenylalanine; D004950:Esterases; D002403:Cathepsins; D012697:Serine Endopeptidases; C534094:CTSG protein, human; D056649:Cathepsin G; C534097:Ctsg protein, rat
Opredelenie ésteraznoĭ aktivnosti serinovykh proteinaz cheloveka i zhivotnykh s ispol'zovaniem v kachestve substratov fliuorogennykh éfirov aminokislot.