title: Experimental evaluation of antitoxic protective effect of new cholera vaccines in mice.
abstract: Intraperitoneal immunization of mice and subsequent challenge with purified cholera toxin (CT) were employed to evaluate the anti-cholera toxin protective effect of two new oral cholera vaccines, live CVD 103-HgR and killed B subunit-whole cell (BS-WC). CVD 103-HgR vaccine demonstrated 100% protection of mice against 2.25 LD50 and 70% against 3 LD50 of CT. Mice immunized with BS-WC vaccine were protected against 2.25 and 3 LD50 of CT in 88 and 62% of cases, respectively. All three killed parenteral vaccines failed to protect against CT. We suggest this mouse system for preliminary evaluation of the antitoxic protective activity of cholera vaccines.
authors.affiliation: Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, MD 20892.
authors.forename: E M
authors.initials: EM
authors.lastname: Dragunsky
authors.forename: E
authors.initials: E
authors.lastname: Rivera
authors.forename: W
authors.initials: W
authors.lastname: Aaronson
authors.forename: T M
authors.initials: TM
authors.lastname: Dolgaya
authors.forename: H D
authors.initials: HD
authors.lastname: Hochstein
authors.forename: W H
authors.initials: WH
authors.lastname: Habig
authors.forename: I S
authors.initials: IS
authors.lastname: Levenbook
mesh_terms: D000818:Animals; D000992:Antitoxins / Q000627:therapeutic use*; D002771:Cholera / Q000517:prevention & control*; D002772:Cholera Toxin / Q000008:administration & dosage / Q000037:antagonists & inhibitors*; D022121:Cholera Vaccines / Q000627:therapeutic use*; D004353:Drug Evaluation, Preclinical; D007114:Immunization; D007274:Injections, Intraperitoneal; D007928:Lethal Dose 50; D008297:Male; D051379:Mice
chemical_list: D000992:Antitoxins; D022121:Cholera Vaccines; D002772:Cholera Toxin