title: The role of the TATA-binding protein in the assembly and function of the multisubunit yeast RNA polymerase III transcription factor, TFIIIB.
abstract: The Saccharomyces cerevisiae RNA polymerase III transcription factor (TF)IIIB has been assembled from three components. An assembly pathway of these polypeptides, which specifies their interactions, has been determined. The TATA-binding protein, TBP, and the TFIIB-related BRF1 gene product BRF, together reconstitute the transcription factor activity and TFIIC-dependent DNA-binding activity of the B' component of TFIIIB. BRF alone weakly binds to a TFIIIC-tRNA gene complex; TBP greatly stabilizes this interaction. B" transcription factor activity is recovered with its previously identified 90 kd polypeptide from SDS-polyacrylamide gels. Incorporation of the 90 kd B" protein into the transcription complex requires TBP. The heparin-resistant TFIIIB-DNA complex retains all three of its constituent proteins, TBP, BRF, and B".
authors.affiliation: Department of Biology, University of California, San Diego, La Jolla 92093-0634.
authors.forename: G A
authors.initials: GA
authors.lastname: Kassavetis
authors.forename: C A
authors.initials: CA
authors.lastname: Joazeiro
authors.forename: M
authors.initials: M
authors.lastname: Pisano
authors.forename: E P
authors.initials: EP
authors.lastname: Geiduschek
authors.forename: T
authors.initials: T
authors.lastname: Colbert
authors.forename: S
authors.initials: S
authors.lastname: Hahn
authors.forename: J A
authors.initials: JA
authors.lastname: Blanco
mesh_terms: D001483:Base Sequence; D004268:DNA-Binding Proteins / Q000378:metabolism*; D008969:Molecular Sequence Data; D011487:Protein Conformation; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D035181:TATA-Box Binding Protein; D035361:Transcription Factor TFIIIB; D014157:Transcription Factors / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D004268:DNA-Binding Proteins; D035181:TATA-Box Binding Protein; D035361:Transcription Factor TFIIIB; D014157:Transcription Factors