title: [Microencapsulation in Na-alginate and in vitro development of sheep blastomeres].
abstract: Embryos at 4 cell stage obtained from Sarda ewes superovulated with FSHp (Sigma) were micromanipulated in order to obtain single blastomeres (1/4 E). The 1/4 E have been located randomly in two groups. In the first (Group A n. 30) the 1/4 E have been put back in empty zonae pellucidae; in the second (Group B n. 21) they have been microencapsulated in sodium alginate (1.1%) by dropping cell-alginate solution in a 1.5% CaCl2. Each capsule (1 mm diameter) contained four 1/4 E. The blastomeres have been co-cultured for 5 days in CZB medium on oviductal cell monolayer in a humidified incubator (5% CO2, 95% air, 38.5 degrees C). No differences were found between the groups reaching blastocyst stage after the end of the culture period (A 50%-B 47%).
authors.affiliation: Istituto Zootecnico e Caseario per la Sardegna, Olmedo, Sassari.
authors.forename: P
authors.initials: P
authors.lastname: Loi
authors.forename: S
authors.initials: S
authors.lastname: Ledda
authors.forename: M
authors.initials: M
authors.lastname: Gallus
authors.forename: F
authors.initials: F
authors.lastname: Filia
authors.forename: P
authors.initials: P
authors.lastname: Cappai
authors.forename: S
authors.initials: S
authors.lastname: Naitana
mesh_terms: D000464:Alginates; D000818:Animals; D001757:Blastomeres / Q000502:physiology*; D046508:Culture Techniques / Q000379:methods*; D004339:Drug Compounding; D005314:Embryonic and Fetal Development / Q000502:physiology*; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D008846:Micromanipulation; D012756:Sheep / Q000196:embryology*; D015044:Zona Pellucida / Q000502:physiology*
chemical_list: D000464:Alginates; D006603:Hexuronic Acids; D020723:Glucuronic Acid
vernacular_title: Microincapsulazione in Na-alginato e sviluppo in vitro di blastomeri di ovino.