Design and inhibitory properties of synthetic Bowman-Birk loops.
A cyclic tridecapeptide based on the sequence of an anti-tryptic loop of a Bowman-Birk inhibitor was synthesized, and demonstrated to be active as an inhibitor of trypsin. Molecular modeling of this sequence suggested an improved sequence which demonstrated an order of magnitude improvement in the inhibitory constant.
Maeder
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Department of Biochemistry, University of Cape Town, Rondebosch, Republic of South Africa.
Sunde
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Botes
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D000595:Amino Acid Sequence; D001667:Binding, Competitive; D007700:Kinetics; D008958:Models, Molecular; D008961:Models, Structural; D008969:Molecular Sequence Data; D015394:Molecular Structure; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D011487:Protein Conformation; D012694:Serine / Q000737:chemistry; D013329:Structure-Activity Relationship; D013912:Threonine / Q000737:chemistry; D014358:Trypsin Inhibitor, Bowman-Birk Soybean / Q000138:chemical synthesis / Q000494:pharmacology*
D010446:Peptide Fragments; D014358:Trypsin Inhibitor, Bowman-Birk Soybean; D013912:Threonine; D012694:Serine