The possibilities of changing the production of exocellular polysaccharides of a mutant Mycobacterium strain.
By second-step mutagenesis and treatment with N-methyl-N'-nitro-N-nitrosoguanidine a mutant strain of Mycobacterium sp. V-649 producing a glucan extracellular polymer and another new streptomycin-resistant mutant were prepared. This mutant strain formed more than 100% first-rate (1.0-1.2%) exocellular polysaccharide. Treatment with 1% dimethyl sulfoxide during submerged cultivation of the mutant strain did not increase the production of the extracellular polysaccharide.
Konícek
J
J
Institute of Microbiology, Czechoslovak Academy of Sciences, Prague.
Lasík
J
J
D003470:Culture Media; D004352:Drug Resistance, Microbial / Q000235:genetics; D008769:Methylnitronitrosoguanidine / Q000494:pharmacology; D009154:Mutation; D009161:Mycobacterium / Q000235:genetics / Q000378:metabolism*; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis* / Q000187:drug effects
D003470:Culture Media; D011135:Polysaccharides, Bacterial; D008769:Methylnitronitrosoguanidine