Comparison of estrogen sulfotransferase and pregnenolone sulfotransferase of guinea pig.
Guinea pig adrenal estrogen sulfotransferase from either sex was eluted as a single peak, irrespective of buffer salt concentration, when subjected to fast protein liquid chromatography on gel filtration columns. The same enzyme was consistently eluted in two distinct peaks during chromatofocusing. Adrenal pregnenolone sulfotransferase was eluted during gel filtration in a heterogeneous pattern, dependent on salt concentration. These properties have made possible almost complete separation of the two sulfotransferases in one step, although adrenal estrogen sulfotransferase may possess a minute intrinsic ability to catalyze sulfation of pregnenolone. Pregnenolone sulfotransferase had no measurable activity toward estrone. Pregnenolone sulfotransferase from both sexes yielded variable elution patterns during chromatofocusing. Estrogen sulfotransferase from the adrenal, as well as that of guinea pig chorion, was strongly inhibited by N-ethylmaleimide and to a lesser degree by iodoacetamide and iodoacetate. Adrenal and chorion estrogen sulfotransferases were thermolabile and were activated, although not protected from the effect of heat, by binding to 3'-phosphoadenosine 5'-phosphosulfate. Adrenal pregnenolone sulfotransferase was inhibited only by high concentrations of N-ethylmaleimide and not at all by iodoacetamide or iodoacetate. It was more thermostable than the estrogen sulfotransferase and was not activated by binding to 3'-phosphoadenosine 5'-phosphosulfate.
Glasier
M A
MA
Department of Biochemistry, University of Western Ontario, London, Canada.
Glutek
S M
SM
Hobkirk
R
R
D000818:Animals; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D003600:Cytosol / Q000737:chemistry; D004795:Enzyme Stability; D005260:Female; D006168:Guinea Pigs; D006358:Hot Temperature; D008297:Male; D015238:Sulfotransferases / Q000032:analysis* / Q000037:antagonists & inhibitors; D013466:Sulfurtransferases / Q000032:analysis* / Q000037:antagonists & inhibitors
D013466:Sulfurtransferases; D015238:Sulfotransferases; C036994:pregnenolone sulfotransferase; C020122:estrone sulfotransferase