Thin-layer electrophoresis with PhastSystem facilitates analysis of phosphoamino acids from proteins bound to Immobilon.
A modification of thin-layer electrophoresis of phosphoamino acids on miniplates, using the PhastSystem (Pharmacia) is described. This method not only reduces the amount of radioactivity required but also the radioactive waste. In addition, it also decreases the handling of hazardous substances and takes only a fraction of the time required for the standard procedure.
Lippmann
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C
Department of Biochemistry, Freie Universität Berlin.
Lindschau
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C
Erdmann
V A
VA
D000596:Amino Acids / Q000032:analysis*; D000818:Animals; D004586:Electrophoresis / Q000295:instrumentation / Q000379:methods*; D005069:Evaluation Studies as Topic; D010710:Phosphates / Q000032:analysis; D010750:Phosphoproteins / Q000737:chemistry; D051381:Rats
D000596:Amino Acids; D010710:Phosphates; D010750:Phosphoproteins