title: Chemical contrast in X-ray microscopy and spatially resolved XANES spectroscopy of organic specimens.
abstract: The scanning transmission x-ray microscope at the National Synchrotron Light Source has been used to record x-ray absorption near-edge structure (XANES) spectra from 0.01-square-micrometer regions of organic specimens. The spectral features observed reflect the molecular structure of the dominant absorbing atoms and provide the contrast mechanism for high-resolution imaging with chemical sensitivity. This technique was used with x-ray energies near the carbon K absorption edge to identify and map separate phases in various polymer blends and to map the DNA distribution in chromosomes with a spatial resolution of 55 nanometers.
authors.affiliation: Department of Physics, State University of New York, Stony Brook, NY 11794.
authors.forename: H
authors.initials: H
authors.lastname: Ade
authors.forename: X
authors.initials: X
authors.lastname: Zhang
authors.forename: S
authors.initials: S
authors.lastname: Cameron
authors.forename: C
authors.initials: C
authors.lastname: Costello
authors.forename: J
authors.initials: J
authors.lastname: Kirz
authors.forename: S
authors.initials: S
authors.lastname: Williams
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry; D002875:Chromosomes / Q000737:chemistry; D004247:DNA / Q000032:analysis / Q000737:chemistry; D007887:Fabaceae / Q000235:genetics; D008853:Microscopy / Q000379:methods*; D010946:Plants, Medicinal; D011126:Polypropylenes / Q000737:chemistry; D011137:Polystyrenes / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000032:analysis / Q000737:chemistry; D013057:Spectrum Analysis / Q000379:methods*; D014965:X-Rays
chemical_list: D000180:Acrylic Resins; D011126:Polypropylenes; D011137:Polystyrenes; C010504:polyacrylonitrile; D012710:Serum Albumin, Bovine; D004247:DNA