title: Use of capillary zone electrophoresis to evaluate the binding of anionic carbohydrates to synthetic peptides derived from human serum amyloid P component.
abstract: Capillary zone electrophoresis was used to study interactions between anionic carbohydrates and synthetic peptides derived from the heparin-binding region of human serum amyloid P component. The method involves quantitation of unbound peptides after a charge-dependent electrophoretic separation of the peptide-carbohydrate mixture. The concentrations of free peptide were determined by extrapolating the obtained peak areas of the peptide in the presence of ligand to a standard curve. Dissociation constants in the 10(-5) M range were determined, and differences in binding affinity of various peptide modifications were illustrated. The assay requires minute amounts of material (sample volume is 7-15 nL), and as long as the reactants are soluble at the chosen conditions, no modifications or special characteristics of the interacting molecules are needed for their identification. It should be possible to use electrophoretic separation in capillaries to evaluate the binding of peptides to any ligand as long as the differences in charge/mass ratio between free and complexed peptide are of a sufficient magnitude as in the peptide-heparin binding demonstrated here.
authors.affiliation: Peptide and Immunochemistry Unit, National Institute of Dental Research, National Institutes of Health, Bethesda, Maryland 20892.
authors.forename: N H
authors.initials: NH
authors.lastname: Heegaard
authors.forename: F A
authors.initials: FA
authors.lastname: Robey
mesh_terms: D000595:Amino Acid Sequence; D000838:Anions; D050260:Carbohydrate Metabolism; D004586:Electrophoresis / Q000379:methods*; D006801:Humans; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000378:metabolism*; D011485:Protein Binding; D000685:Serum Amyloid A Protein / Q000378:metabolism*
chemical_list: D000838:Anions; D010446:Peptide Fragments; D000685:Serum Amyloid A Protein