Isolation of peroxisomes from frozen human liver samples.
This paper shows the successful isolation of peroxisomes from human liver samples that were kept frozen at -70 degrees C. Purification of these peroxisomes was obtained by a combination of two subcellular fractionation techniques: differential centrifugation and isopycnic fractionation in Nycodenz density gradients. Peroxisome integrity was evaluated by latency measurements and by ultrastructural observation. The procedure described here may be useful for the isolation of other subcellular organelles from frozen human samples.
Alvarez
A
A
Department of Cell & Molecular Biology, Faculty of Biological Sciences, Catholic University of Chile, Santiago.
Hidalgo
U
U
Kawada
M E
ME
Munizaga
A
A
Zúñiga
A
A
Ibánez
L
L
Koenig
C S
CS
Santos
M J
MJ
D002458:Cell Fractionation / Q000379:methods; D015925:Cryopreservation; D006651:Histocytochemistry; D006801:Humans; D008099:Liver / Q000201:enzymology / Q000648:ultrastructure*; D008830:Microbodies / Q000648:ultrastructure*; D008854:Microscopy, Electron; D013347:Subcellular Fractions; D014021:Tissue Preservation