Heat stress of cultured GC cells enhances triiodothyronine-induced growth hormone production by action within the 5'-flanking region of the rat growth hormone gene.
We studied the effect of incubation at 41 C on a clone of GC cells that had previously been stably transfected with a gene construct, pGHXGPT, containing -1800 to +8 of the rat growth hormone promoter fused to the structural gene for E. Coli xanthine guanine phosphoribosyl-transferase. The effect of incubation of the clone containing pGHXGPT at 41 C was to enhance triiodothyronine induction of growth hormone secretion (2-fold, p < 0.01) and of xanthine quanine phosphoribosyl-transferase activity (3-fold, p < 0.01). We conclude that the increase in triiodothyronine-induced growth hormone production during heat stress occurs by stimulation of the growth hormone promoter.
Mokshagundam
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Division of Endocrinology & Metabolism, Montefiore Medical Center, Bronx, New York.
Shapiro
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Surks
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D000818:Animals; D002460:Cell Line; D004742:Enhancer Elements, Genetic / Q000187:drug effects*; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D005798:Genes, Bacterial; D013006:Growth Hormone / Q000096:biosynthesis* / Q000235:genetics*; D006358:Hot Temperature; D007700:Kinetics; D010430:Pentosyltransferases / Q000096:biosynthesis / Q000235:genetics; D010957:Plasmids; D011401:Promoter Regions, Genetic / Q000187:drug effects*; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D014162:Transfection; D014284:Triiodothyronine / Q000494:pharmacology*
D011993:Recombinant Fusion Proteins; D014284:Triiodothyronine; D013006:Growth Hormone; D010430:Pentosyltransferases; C024827:xanthine phosphoribosyltransferase