title: Schistosome circulating anodic antigen in serum of individuals infected with Schistosoma japonicum from the Philippines before and after chemotherapy with praziquantel.
abstract: The presence of the schistosome circulating anodic antigen (CAA) in serum of patients infected with Schistosoma japonicum from The Philippines has been investigated using an enzyme-linked immunosorbent assay (ELISA). Serum samples were tested from 48 patients who excreted S. japonicum eggs, 9 individuals with a negative stool examination, and 20 controls with both a negative stool and a negative circumoval precipitin test. No false positive result was detected for the unequivocally negative controls. CAA could be demonstrated in 72.9% of the egg-excreting patients. A positive correlation between parasite burden (eggs per gram of faeces) and antigen level (CAA titre) was found (Spearman's rho = 0.48, P < 0.001, n = 48). Four of 18 sera from the egg-negative individuals were positive in the ELISA. In view of the fact that anti-worm antibodies were also detected in these 4 sera, those reactions suggest active infection not detected by stool examination. In serum from patients treated with praziquantel, a significant drop in CAA titre was seen within 5 d after treatment (Wilcoxon's chi T = -2.23, P = 0.0258, n = 21). In conclusion, the detection of CAA by ELISA in S. japonicum infection can give valuable information in both individual diagnosis and therapeutic drug monitoring, as well as in epidemiological studies or disease control programmes.
authors.affiliation: Laboratory of Parasitology, Medical Faculty, University of Leiden, The Netherlands.
authors.forename: A B
authors.initials: AB
authors.lastname: Van 't Wout
authors.forename: N
authors.initials: N
authors.lastname: De Jonge
authors.forename: W U
authors.initials: WU
authors.lastname: Tiu
authors.forename: E E
authors.initials: EE
authors.lastname: Garcia
authors.forename: G F
authors.initials: GF
authors.lastname: Mitchell
authors.forename: A M
authors.initials: AM
authors.lastname: Deelder
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D000947:Antigens, Helminth / Q000097:blood*; D000953:Antigens, Protozoan; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000469:parasitology; D006801:Humans; D010270:Parasite Egg Count; D010679:Philippines; D011223:Praziquantel / Q000627:therapeutic use*; D012549:Schistosoma japonicum / Q000276:immunology* / Q000302:isolation & purification; D012554:Schistosomiasis japonica / Q000188:drug therapy / Q000276:immunology* / Q000469:parasitology
chemical_list: D000947:Antigens, Helminth; D000953:Antigens, Protozoan; D011223:Praziquantel