title: Secondary structure and interactions of the packaged dsDNA genome of bacteriophage P22 investigated by Raman difference spectroscopy.
abstract: Vibrational spectra of the double-stranded DNA genome of bacteriophage P22 in packaged and unpackaged states are compared by digital difference Raman spectroscopy. The difference Raman spectrum, which is sensitive to structural changes at the level of < 2% of a given nucleotide type, reveals the effects of packaging upon sugar pucker, glycosyl orientation, phosphodiester geometry, base pairing, base stacking, and the electrostatic environment of DNA phosphate groups. For both packaged and unpackaged states, the experiments were performed on aqueous solutions at 25 degrees C containing effective P22 DNA concentrations of 30-50 mg/mL in 200 mM NaCl + 10 mM MgCl2 + 10 mM Tris at pH 7.5. At the experimental conditions employed, the B-form secondary structure of unpackaged P22 DNA is minimally perturbed by packaging the viral genome in the virion capsid. However, the electrostatic environment of DNA phosphates is dramatically altered with packaging. Specifically, we find the following: (1) C2'-endo sugar pucker and anti glycosyl orientations are conserved for all nucleosides. (2) Watson-Crick base pairing is essentially completely retained. (3) Alternative secondary structures, whether right- (A or C form) or left-handed (Z form), are not evident in either the packaged or unpackaged viral genome. (4) Small Raman hyperchromic effects (< 10%) observed for certain marker bands of dG, dA, and dT in the packaged state of P22 DNA suggest slightly reduced base-stacking interactions with packaging. These are consistent with previously reported UV hyperchromic effects, but the Raman spectrum shows that they are not associated with either base unpairing or strand separation.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Division of Cell Biology and Biophysics, School of Biological Sciences, University of Missouri-Kansas City 64110-2499.
authors.forename: K L
authors.initials: KL
authors.lastname: Aubrey
authors.forename: S R
authors.initials: SR
authors.lastname: Casjens
authors.forename: G J
authors.initials: GJ
authors.lastname: Thomas
mesh_terms: D001435:Bacteriophages / Q000235:genetics*; D001482:Base Composition; D001665:Binding Sites; D004279:DNA, Viral / Q000737:chemistry*; D004563:Electrochemistry; D005814:Genes, Viral; D008274:Magnesium / Q000494:pharmacology; D009154:Mutation; D009690:Nucleic Acid Conformation; D010710:Phosphates / Q000737:chemistry; D013059:Spectrum Analysis, Raman
chemical_list: D004279:DNA, Viral; D010710:Phosphates; D008274:Magnesium