title: The influence of oxytocin, vasopressin and their analogues on progesterone and testosterone production by porcine granulosa cells in vitro.
abstract: Effects of nonapeptide hormones and some of their chemical analogues on progesterone and testosterone production by culture of porcine granulosa cells have been investigated. Oxytocin (0.01-10 IU/ml), arginine-8-vasopressin (0.01-10 micrograms/ml), arginine-8-vasotocin (0.01-10 micrograms/ml) and, in a lesser degree, 2-0-methyl-tyrosine (deamino-1-karba)-oxytocin (0.01-10 micrograms/ml, but no 1-deamino-8-vasopressin (0.01-10 micrograms/ml) stimulated a progesterone surge. Testosterone production was significantly stimulated by oxytocin and inhibited by vasopressin or vasotocin additions. 2-0-methyl-tyrosine (deamino-1-karba)-oxytocin or 1-deamino-8-vasopressin had little or no effect on testosterone secretion. The present results suggest the existence of a direct influence of nonapeptide hormones on porcine ovarian progestagen and androgen production.
authors.affiliation: Research Institute of Animal Production, Nitra, Czechoslovakia.
authors.forename: A V
authors.initials: AV
authors.lastname: Sirotkin
authors.forename: J
authors.initials: J
authors.lastname: Nitray
mesh_terms: D000818:Animals; D001127:Arginine Vasopressin / Q000494:pharmacology; D003894:Deamino Arginine Vasopressin / Q000494:pharmacology; D005260:Female; D006107:Granulosa Cells / Q000378:metabolism*; D066298:In Vitro Techniques; D010121:Oxytocin / Q000031:analogs & derivatives / Q000494:pharmacology*; D011374:Progesterone / Q000096:biosynthesis*; D013552:Swine / Q000378:metabolism*; D013739:Testosterone / Q000096:biosynthesis*; D014667:Vasopressins / Q000494:pharmacology*; D014668:Vasotocin / Q000494:pharmacology
chemical_list: D014667:Vasopressins; D001127:Arginine Vasopressin; D013739:Testosterone; D011374:Progesterone; D010121:Oxytocin; D003894:Deamino Arginine Vasopressin; D014668:Vasotocin