title: An easy and fast alternative to plasmid shuffling for the identification of in vitro mutagenized alleles of essential genes of Saccharomyces cerevisiae.
authors.affiliation: Physiologisch-chemisches Institut, Tübingen, Germany.
authors.forename: K U
authors.initials: KU
authors.lastname: Fröhlich
authors.forename: M
authors.initials: M
authors.lastname: Rüdiger
authors.forename: D
authors.initials: D
authors.lastname: Eberhardt
authors.forename: D
authors.initials: D
authors.lastname: Mecke
mesh_terms: D000251:Adenosine Triphosphatases; D000483:Alleles; D018797:Cell Cycle Proteins; D005656:Fungal Proteins / Q000235:genetics; D005800:Genes, Fungal; D005821:Genetic Techniques; D016296:Mutagenesis; D010957:Plasmids; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000302:isolation & purification; D029701:Saccharomyces cerevisiae Proteins; D000074405:Valosin Containing Protein
chemical_list: D018797:Cell Cycle Proteins; D005656:Fungal Proteins; D029701:Saccharomyces cerevisiae Proteins; D000251:Adenosine Triphosphatases; C000614177:CDC48 protein, S cerevisiae; D000074405:Valosin Containing Protein