title: Characterization of bovine heparin-binding neurotrophic factor (HBNF): assignment of disulfide bonds.
abstract: The topology of the disulfides in native heparin-binding neurotrophic factor (HBNF) isolated from bovine brain was studied by proteolytic digestion using trypsin, Asp-N endoproteinase and chymotrypsin and peptide mapping. Disulfide-linked peptides were identified by automated Edman degradation. It has been shown that there are disulfide bonds between Cys15-Cys44, Cys23-Cys53, Cys30-Cys57, Cys67-Cys99 and Cys77-Cys109.
authors.affiliation: Ludwig Institute for Cancer Research (Melbourne Branch), Parkville, Victoria, Australia.
authors.forename: L
authors.initials: L
authors.lastname: Fabri
authors.forename: E C
authors.initials: EC
authors.lastname: Nice
authors.forename: L D
authors.initials: LD
authors.lastname: Ward
authors.forename: H
authors.initials: H
authors.lastname: Maruta
authors.forename: A W
authors.initials: AW
authors.lastname: Burgess
authors.forename: R J
authors.initials: RJ
authors.lastname: Simpson
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001923:Brain Chemistry; D002352:Carrier Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D002417:Cattle; D002918:Chymotrypsin; D003546:Cysteine Endopeptidases; D016207:Cytokines / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D004220:Disulfides / Q000737:chemistry; D010450:Endopeptidases; D008969:Molecular Sequence Data; D015394:Molecular Structure; D008970:Molecular Weight; D010449:Peptide Mapping; D017433:Protein Structure, Secondary; D014357:Trypsin; D000099240:Asparaginyl Endopeptidase
chemical_list: D002352:Carrier Proteins; D002918:Chymotrypsin; D003546:Cysteine Endopeptidases; D016207:Cytokines; D004220:Disulfides; D010450:Endopeptidases; D014357:Trypsin; D000099240:Asparaginyl Endopeptidase; C066845:pleiotrophin