Amniotic fluid embolism and leukotrienes--the role of amniotic fluid surfactant in leukotriene production.
Surfactant rich lipid (lipid) was extracted from cell free 10,000 x g pellets of amniotic fluid. White blood cells (WBC) were isolated from human donors. 36 x 10(7) WBC and 5 g rabbit lung were incubated with pretreated lipid or dipalmitoyl lecithin (lecithin). Leukotrienes (LTs) were identified by high performance liquid chromatography (HPLC) and bioassay, and quantified by radioimmunoassay. Peaks of LTC4 and LTD4 on HPLC and guinea-pig ileum contraction could be identified in lipid and lecithin groups, but not in the control group. LTC4 production by lipid and lecithin groups was significantly higher than that by the control group. An involvement of amniotic fluid surfactant in leukotriene production is suggested.
Lee
H C
HC
Department of Obstetrics and Gynecology, Miyazaki Medical College, Japan.
Yamaguchi
M
M
Ikenoue
T
T
Miyakawa
I
I
Mori
N
N
D015060:1,2-Dipalmitoylphosphatidylcholine / Q000494:pharmacology; D000653:Amniotic Fluid / Q000737:chemistry*; D000818:Animals; D001681:Biological Assay; D002851:Chromatography, High Pressure Liquid; D004619:Embolism, Amniotic Fluid / Q000503:physiopathology*; D005260:Female; D006168:Guinea Pigs; D006801:Humans; D007962:Leukocytes / Q000187:drug effects*; D015289:Leukotrienes / Q000096:biosynthesis*; D008168:Lung / Q000187:drug effects*; D009119:Muscle Contraction / Q000187:drug effects; D009130:Muscle, Smooth / Q000187:drug effects; D011247:Pregnancy; D011817:Rabbits; D013501:Surface-Active Agents / Q000302:isolation & purification / Q000494:pharmacology*
D015289:Leukotrienes; D013501:Surface-Active Agents; D015060:1,2-Dipalmitoylphosphatidylcholine