title: Yeast RNA polymerase II initiation factor e: isolation and identification as the functional counterpart of human transcription factor IIB.
abstract: Yeast RNA polymerase II initiation factor e was purified to homogeneity and identified by biochemical criteria as the counterpart of human transcription factor IIB. Factor e was essential for initiation of transcription from yeast and mammalian promoters in a reconstituted yeast transcription system. Activity resided in a single polypeptide of approximately 41 kDa, identified by peptide sequence analysis as the product of the SUA7 gene. Factor e interacted specifically with RNA polymerase II, consistent with a proposed role in determining the start site of transcription.
authors.affiliation: Department of Cell Biology, Fairchild Center, Stanford University School of Medicine, CA 94305.
authors.forename: H
authors.initials: H
authors.lastname: Tschochner
authors.forename: M H
authors.initials: MH
authors.lastname: Sayre
authors.forename: P M
authors.initials: PM
authors.lastname: Flanagan
authors.forename: W J
authors.initials: WJ
authors.lastname: Feaver
authors.forename: R D
authors.initials: RD
authors.lastname: Kornberg
mesh_terms: D000595:Amino Acid Sequence; D005656:Fungal Proteins / Q000302:isolation & purification* / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry; D011401:Promoter Regions, Genetic; D012319:RNA Polymerase II / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D012568:Schizosaccharomyces / Q000235:genetics; D035561:Transcription Factor TFIIA; D014157:Transcription Factors / Q000302:isolation & purification* / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D005656:Fungal Proteins; D010446:Peptide Fragments; D012333:RNA, Messenger; D035561:Transcription Factor TFIIA; D014157:Transcription Factors; D012319:RNA Polymerase II