[The production of "pure" cultures of neurons from the brain of human embryos].
A method has been developed for preparing essentially pure primary cultures of neurons from 10-12 week old fetal human brain. This method is based on the 1) capacity of neurons to form multicellular aggregates, 2) cultivation in chemically defined medium, and 3) treatment with cytosine arabinoside as antimitotic agent. This procedure allowed the preparation of highly purified (95 per cent and more) neuronal aggregate cultures. Ultrastructural analysis of these cultures following one and 8 days has revealed their partial differentiation during cultivation.
Balabanov
Iu V
IuV
Pavlov
O V
OV
Bobryshev
Iu V
IuV
D001921:Brain / Q000378:metabolism / Q000648:ultrastructure*; D002449:Cell Aggregation; D002454:Cell Differentiation; D002478:Cells, Cultured / Q000378:metabolism / Q000648:ultrastructure; D016895:Culture Media, Serum-Free; D003561:Cytarabine / Q000494:pharmacology; D003584:Cytological Techniques; D004622:Embryo, Mammalian; D005865:Gestational Age; D006801:Humans; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D008938:Mitosis / Q000187:drug effects; D009474:Neurons / Q000378:metabolism / Q000648:ultrastructure*
D016895:Culture Media, Serum-Free; D003561:Cytarabine
Poluchenie "chistykh" kul'tur neĭronov golovnogo mozga émbrionov cheloveka.