Ligands induce conformational changes in the carboxyl-terminus of progesterone receptors which are detected by a site-directed antipeptide monoclonal antibody.
We have prepared a monoclonal antibody, C-262, to a synthetic peptide that contains the carboxy-terminal 14 amino acids from progesterone receptors (PR). This sequence is 100% conserved in all species of PRs that have been cloned to date, suggesting that this antibody will recognize all mammalian and avian PR. The C-262 antibody recognizes both native and denatured forms of the receptor. However, it does not recognize PR when they are bound to the hormone agonists progesterone or R5020. Surprisingly the antibody does recognize PR when they are bound to the steroid antagonist RU486. This suggests that progestin agonists induce a conformational change in the receptor that occludes the C-262 epitope in the carboxyl-terminus, whereas unliganded receptors and receptors bound with RU486 assume distinct conformations that leaves the C-terminal tail accessible to the C-262 antibody.
Weigel
N L
NL
Baylor College of Medicine, Cell Biology Department, Houston, Texas 77030.
Beck
C A
CA
Estes
P A
PA
Prendergast
P
P
Altmann
M
M
Christensen
K
K
Edwards
D P
DP
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D001665:Binding Sites; D015153:Blotting, Western; D001943:Breast Neoplasms; D002645:Chickens; D003600:Cytosol / Q000378:metabolism; D005260:Female; D006801:Humans; D008024:Ligands; D015735:Mifepristone / Q000378:metabolism*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010057:Oviducts / Q000378:metabolism*; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology; D011487:Protein Conformation; D011980:Receptors, Progesterone / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D008024:Ligands; D010455:Peptides; D011980:Receptors, Progesterone; D015735:Mifepristone