Fresh and frozen-thawed human sperm bind in a similar pattern to the zona pellucida in the hemizona assay.
This study evaluated the impact of sperm cryopreservation on sperm quality. The HZA was used to test the binding capacity of fresh as opposed to frozen-thawed sperm from 12 donors. Fresh and frozen-thawed sperm motility was 47% +/- 1.5% and 24% +/- 3.8% (mean +/- SE), respectively. However, the number of sperm cells attached to the hemizonae was 75 +/- 12.0 and 74 +/- 11.9, respectively. We conclude that cryopreservation results in a reduced number of motile sperm cells but does not adversely affect the ability of rescued sperm cells to bind to the ZP. The study also supports the use of frozen-thawed rather than fresh donor sperm for control in the HZA procedure.
Gamzu
R
R
Institute for the Study of Fertility, Serlin Maternity Hospital, Tel Aviv, Israel.
Yogev
L
L
Yavetz
H
H
Homonnai
Z T
ZT
Hiss
Y
Y
Paz
G
G
D015925:Cryopreservation; D005260:Female; D006358:Hot Temperature; D006801:Humans; D008297:Male; D013084:Sperm-Ovum Interactions; D013094:Spermatozoa / Q000502:physiology*; D015044:Zona Pellucida / Q000378:metabolism*