The anti-asthmatic agent KC 404 inhibits sodium absorption by canine tracheal epithelium.
To elucidate the effect of KC 404, an anti-asthmatic agent, on electrolyte transport across airway mucosa, we studied electrical properties of canine tracheal epithelium under isometric conditions in vitro. Addition of KC 404 decreased short-circuit current, transepithelial potential difference and cell conductance, accompanied by the decrease in 22Na flux from submucosa to mucosa. The KC 404-induced decrease in short-circuit current was inhibited by amiloride but not by diphenylamine-2-carboxylate. These results suggest that KC 404 selectively inhibits Na absorption by airway epithelium, thereby leading to the increase in water content in the respiratory lumen.
Tamaoki
J
J
First Department of Medicine, Tokyo Women's Medical College, Japan.
Isono
K
K
Sakai
N
N
Chiyotani
A
A
Konno
K
K
D000042:Absorption; D000584:Amiloride / Q000494:pharmacology; D000818:Animals; D001249:Asthma / Q000188:drug therapy; D001693:Biological Transport, Active / Q000187:drug effects; D004285:Dogs; D004563:Electrochemistry; D004848:Epithelium / Q000187:drug effects / Q000378:metabolism; D005260:Female; D066298:In Vitro Techniques; D008297:Male; D011725:Pyridines / Q000494:pharmacology*; D012964:Sodium / Q000378:metabolism*; D014132:Trachea / Q000187:drug effects* / Q000378:metabolism; D007212:Indolizines; D011720:Pyrazoles
D000584:Amiloride; D011725:Pyridines; D012964:Sodium; C038366:ibudilast; D007212:Indolizines; D011720:Pyrazoles