title: Studies on the methanolysis of small amounts of purified phospholipids for gas chromatographic analysis of fatty acid methyl esters.
abstract: The methanolysis of small amounts of purified phosphoglycerides and sphingomyelin was studied and a quantitative comparison of five methods for the methanolysis of standard phosphoglycerides was made. These methods were based on methanolysis with boron trifluoride-methanol, methanolic sodium methoxide (at ambient temperature and with heating) and methanolic sulphuric acid. A further method was based on saponification with methanolic sodium hydroxide and subsequent esterification with boron trifluoride-methanol. Under the experimental conditions, only the sodium methoxide-catalysed method at ambient temperature gave complete methanolysis of phosphoglycerides. For methanolysis of sphingomyelin, boron trifluoride-methanol, methanolic sulphuric acid and methanolic hydrochloric acid were used. It was found that complete methanolysis of sphingomyelin takes 15 h at 90 degrees C. Based on these results, procedures for the methanolysis of phosphoglycerides and sphingomyelin separated by high-performance liquid chromatography are presented.
authors.affiliation: Institut für Ernährungsphysiologie, Technische Universität München,Freising-Weihenstephan, Germany.
authors.forename: K
authors.initials: K
authors.lastname: Eder
authors.forename: A M
authors.initials: AM
authors.lastname: Reichlmayr-Lais
authors.forename: M
authors.initials: M
authors.lastname: Kirchgessner
mesh_terms: D000818:Animals; D001880:Boranes; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D004531:Eggs / Q000032:analysis; D004910:Erythrocyte Membrane / Q000737:chemistry; D005260:Female; D005994:Glycerophosphates / Q000737:chemistry; D006868:Hydrolysis; D000432:Methanol; D010743:Phospholipids / Q000032:analysis*; D010955:Plasmalogens / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D013109:Sphingomyelins / Q000737:chemistry; D013464:Sulfuric Acids / Q000737:chemistry
chemical_list: D001880:Boranes; D005994:Glycerophosphates; D010743:Phospholipids; D010955:Plasmalogens; D013109:Sphingomyelins; D013464:Sulfuric Acids; C021274:boron trifluoride; D000432:Methanol