title: Lactoferrin binding sites and nuclear localization in K562(S) cells.
abstract: Lactoferrin, a single chain cationic glycoprotein, present in the secondary granules of neutrophils, acts as a negative feedback regulator of myelopoiesis. Specific receptors for lactoferrin were detected on the surface of different hematopoietic cell types. The influence of lactoferrin on cell growth in culture has been reported. Interactions of lactoferrin with DNA were also demonstrated. In the present paper we confirm the presence of lactoferrin specific binding sites on K562 cells and we estimate the number of binding sites and the dissociation constant. By Western blotting analysis performed on K562 lysates we find a band of about 120 kDa responsible for specific binding of lactoferrin. We also show that lactoferrin, after binding at the cell surface, is internalized in a temperature dependent way and is immunologically detectable as a DNA-linked protein in nuclear extracts.
authors.affiliation: Institute of Biology and Genetics, University of Genova, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Garré
authors.forename: G
authors.initials: G
authors.lastname: Bianchi-Scarrá
authors.forename: M
authors.initials: M
authors.lastname: Sirito
authors.forename: M
authors.initials: M
authors.lastname: Musso
authors.forename: R
authors.initials: R
authors.lastname: Ravazzolo
mesh_terms: D001665:Binding Sites; D015153:Blotting, Western; D002467:Cell Nucleus / Q000378:metabolism*; D003432:Cross-Linking Reagents; D004247:DNA / Q000378:metabolism; D006801:Humans; D007781:Lactoferrin / Q000378:metabolism*; D013997:Time Factors; D014018:Tissue Distribution; D014407:Tumor Cells, Cultured
chemical_list: D003432:Cross-Linking Reagents; D004247:DNA; D007781:Lactoferrin