Trehalase from male accessory gland of an insect, Tenebrio molitor. cDNA sequencing and developmental profile of the gene expression.
A cDNA of alpha alpha-trehalase (EC 3.2.1.28) from a cDNA library of male bean-shaped accessory gland of the mealworm beetle, Tenebrio molitor, has been isolated by the homology screening approach. Sequence analysis of the cDNA (1830 bp) revealed that the cDNA encoded a protein of 555 amino acids with a calculated M(r) of 64457. The deduced amino acid sequence had significant similarities to rabbit small intestine and Escherichia coli trehalases. Northern blotting and semi-quantitative PCR analyses revealed that a trehalase transcript with about 2.0 kb was abundant in bean-shaped accessory glands. In the glands, the amount of trehalase transcript increased from 1 to 2 days after adult ecdysis. These tissue- and stage-specific gene expressions of trehalase corresponded to the tissue- and stage-specificity of trehalase activity.
Takiguchi
M
M
Laboratory of Sericultural Science, Faculty of Agriculture, Nagoya University, Japan.
Niimi
T
T
Su
Z H
ZH
Yaginuma
T
T
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D004247:DNA / Q000737:chemistry / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology; D015870:Gene Expression; D005837:Genitalia, Male / Q000201:enzymology; D008297:Male; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D011817:Rabbits; D017386:Sequence Homology, Amino Acid; D013712:Tenebrio / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D014198:Trehalase / Q000737:chemistry / Q000235:genetics*
D004247:DNA; D014198:Trehalase