Differential release of human intestinal alkaline phosphatase in duodenal fluid and serum.
Human intestinal alkaline phosphatase (IAP) can be released by the enterocyte into duodenal fluid as a mixture of three isoforms. A proportion of the enzyme is associated with triple-layered membrane vesicles (vesicular IAP). Although, occasionally, free hydrophilic IAP dimers are present, the remaining enzyme usually consists of a mixture of hydrophobic IAP dimers and more complex hydrophobic IAP structures of larger size, both entities being identified as "intestinal variant" alkaline phosphatase (VAR IAP). The hydrophobicity of VAR IAP stems exclusively from its attached glycosyl-phosphatidylinositol (GPI) anchor. Both vesicular IAP and VAR IAP are converted to hydrophilic enzyme upon removal of the GPI tail by phospholipase D (PLD) present in duodenal fluid. The IAP released into the vascular bed consists mainly of VAR IAP; vesicular IAP is absent. The enzyme characteristics of VAR IAP partially purified from duodenal fluid and from serum are identical. In plasma, VAR IAP appears to associate with (lipo)protein complexes and is thus protected from further degradation by plasma PLD. Such complex formation may explain why, in the serum of a healthy reference population, VAR IAP was more abundant than hydrophilic dimeric IAP.
Deng
J T
JT
Department of Nephrology-Hypertension, University of Antwerp, Belgium.
Hoylaerts
M F
MF
Van Hoof
V O
VO
De Broe
M E
ME
D000469:Alkaline Phosphatase / Q000097:blood / Q000737:chemistry / Q000378:metabolism*; D001826:Body Fluids / Q000201:enzymology*; D002850:Chromatography, Gel; D003902:Detergents / Q000494:pharmacology; D004386:Duodenum / Q000201:enzymology*; D004591:Electrophoresis, Polyacrylamide Gel; D014644:Genetic Variation; D006801:Humans; D007422:Intestines / Q000201:enzymology*; D007525:Isoelectric Focusing; D007527:Isoenzymes / Q000097:blood / Q000737:chemistry / Q000378:metabolism*; D046911:Macromolecular Substances; D010739:Phospholipase D / Q000378:metabolism
D003902:Detergents; D007527:Isoenzymes; D046911:Macromolecular Substances; D000469:Alkaline Phosphatase; D010739:Phospholipase D