Activation of cationic amino acid transport through system y+ correlates with expression of the T-cell early antigen gene in human lymphocytes.
Lysine influx (2 microM) into activated human B and T lymphocytes through transport systems y+ and y+L was measured. In activated T cells very substantial activation of system y+ was detected; system y+L was also activated in these cells but with a slower time course and to a smaller extent. No stimulation of either system was found in activated B cells. The time course of activation of system y+ precisely matched the expression of the T cell early antigen gene described by MacLeod et al, 1990. The functional significance of these observations with respect to L-arginine transport and nitric oxide synthesis in activated T lymphocytes is discussed.
Boyd
C A
CA
Department of Human Anatomy, University of Oxford, UK.
Crawford
D H
DH
D000596:Amino Acids / Q000378:metabolism*; D000945:Antigens, Differentiation, T-Lymphocyte / Q000235:genetics*; D001402:B-Lymphocytes / Q000378:metabolism; D001692:Biological Transport; D002412:Cations / Q000378:metabolism*; D015870:Gene Expression; D006801:Humans; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000502:physiology*; D008239:Lysine / Q000493:pharmacokinetics; D013601:T-Lymphocytes / Q000378:metabolism
D000596:Amino Acids; D000945:Antigens, Differentiation, T-Lymphocyte; D002412:Cations; D008239:Lysine