The presence of neuropeptides in GABAergic and cholinergic rat cerebrocortical synaptosome sub-populations.
GABAergic and cholinergic synaptosomes from rat cerebral cortex were isolated by a magnetic immunoaffinity technique, i.e. immunomagnetophoresis. These subpopulations were extracted and subjected to radioimmunoassay for four neuropeptides: Neuropeptide Y (NPY); vasoactive intestinal peptide (VIP); substance P (SP); and somatostatin (SRIF). In each of the sub-populations three of the four peptides were enriched in the sorted fraction compared with the mother fraction with respect to the cytosolic marker lactate dehydrogenase (LDH). In the GABAergic sub-population the order was SP > SRIF > NPY > or = VIP whilst in the cholinergic sub-population they were enriched in the order VIP > or = NPY > SP > SRIF. The presence of NPY has not previously been reported in cortical cholinergic neurons.
Foley
P
P
Department of Biochemistry, Imperial College of Science, Technology & Medicine, South Kensington, London, UK.
Hughes
P D
PD
Bradford
H F
HF
Ghatei
M A
MA
Khandanian
N
N
Bloom
S R
SR
D000818:Animals; D015415:Biomarkers; D001923:Brain Chemistry / Q000187:drug effects; D002540:Cerebral Cortex / Q000378:metabolism*; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D009479:Neuropeptides / Q000378:metabolism*; D010275:Parasympathetic Nervous System / Q000378:metabolism*; D011863:Radioimmunoassay; D051381:Rats; D013574:Synaptosomes / Q000378:metabolism*; D005680:gamma-Aminobutyric Acid / Q000502:physiology*
D015415:Biomarkers; D009479:Neuropeptides; D005680:gamma-Aminobutyric Acid