title: In vitro translation of a subgenomic mRNA from purified virions of the Spanish field isolate AST/89 of rabbit hemorrhagic disease virus (RHDV).
abstract: Purified preparations of the Spanish field isolate of rabbit hemorrhagic disease virus AST/89 were found to contain the plus-stranded genomic RNA of more than 7.4 kilobases (kb) and large amounts of a subgenomic mRNA of 2.4 kb. The smaller RNA was translated in vitro and shown to code for a 60 kDa protein which was immunoprecipitated using anti-RHDV as well as anti-VP60 sera.
authors.affiliation: Departamento de Biología Funcional (Area de Bioquímica), Universidad de Oviedo, Spain.
authors.forename: J A
authors.initials: JA
authors.lastname: Boga
authors.forename: M S
authors.initials: MS
authors.lastname: Marín
authors.forename: R
authors.initials: R
authors.lastname: Casais
authors.forename: M
authors.initials: M
authors.lastname: Prieto
authors.forename: F
authors.initials: F
authors.lastname: Parra
mesh_terms: D002139:Caliciviridae / Q000235:genetics* / Q000302:isolation & purification; D002474:Cell-Free System; D016679:Genome, Viral; D014176:Protein Biosynthesis; D015347:RNA Probes; D012333:RNA, Messenger / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics; D014771:Virion / Q000235:genetics / Q000302:isolation & purification
chemical_list: D015347:RNA Probes; D012333:RNA, Messenger; D012367:RNA, Viral; D014764:Viral Proteins