Characterization of activated and normal mouse Mos gene in murine 3T3 cells.
We have characterized the mouse Mos proto-oncogene product, pp39Mos, in murine fibroblasts. When expressed in NIH3T3 cells under the influence of the long terminal repeat regulatory element from Moloney murine sarcoma virus [NIH(pTS-1) cells], the Mos protein was present in low levels and had a half-life of about 30 min. In extracts from NIH(pTS-1) cells, we detected additional forms of Mos protein that apparently arose from internal initiation codons (p24Mos and p29Mos) or from upstream non-AUG initiation codons (p42Mos and p44Mos). The Mos protein was found to exist in these cells as a phosphoprotein, pp39Mos, and, when immunoprecipitated with an antiserum specific for the Mos N-terminus [anti-Mos(6-24)], had autophosphorylating kinase activity. We found that anti-Mos(6-24) also detected non-Mos protein kinase activity and non-Mos phosphoproteins in addition to p39Mos. We present evidence, on both the RNA and protein levels, that non-transformed mouse 3T3 cells do not express endogenous Mos.
Paules
R S
RS
Mammalian Molecular Genetics Group, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709.
Resnick
J
J
Kasenally
A B
AB
Ernst
M K
MK
Donovan
P
P
Vande Woude
G F
GF
D016475:3T3 Cells; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002453:Cell Cycle / Q000187:drug effects; D003062:Codon; D003545:Cysteine / Q000378:metabolism; D016258:Genes, mos; D006918:Hydroxyurea / Q000494:pharmacology; D007700:Kinetics; D008715:Methionine / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D008980:Moloney murine sarcoma virus / Q000235:genetics; D010750:Phosphoproteins / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics*; D010766:Phosphorylation; D011505:Protein-Tyrosine Kinases / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics; D017231:Proto-Oncogene Proteins c-mos / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012045:Regulatory Sequences, Nucleic Acid; D014158:Transcription, Genetic; D014162:Transfection
D003062:Codon; D010750:Phosphoproteins; D012333:RNA, Messenger; D008715:Methionine; D011505:Protein-Tyrosine Kinases; D017231:Proto-Oncogene Proteins c-mos; D003545:Cysteine; D006918:Hydroxyurea