title: Gene targeting using a mouse HPRT minigene/HPRT-deficient embryonic stem cell system: inactivation of the mouse ERCC-1 gene.
abstract: A convenient system for gene targeting that uses hypoxanthine phosphoribosyltransferase (HPRT) minigenes as the selectable marker in HPRT-deficient mouse embryonic stem (ES) cells is described. Improvements to the expression of HPRT minigenes in ES cells were achieved by promoter substitution and the provision of a strong translational initiation signal. The use of minigenes in the positive-negative selection strategy for gene targeting was evaluated and the smaller minigenes were found to be as effective as a more conventional marker--the herpes simplex virus thymidine kinase gene. Minigenes were used to target the DNA repair gene ERCC-1 in ES cells. A new HPRT-deficient ES cell line was developed that contributes with high frequency to the germ line of chimeric animals. The ability to select for and against HPRT minigene expression in the new HPRT-deficient ES cell line will make this system useful for a range of gene-targeting applications.
authors.affiliation: Institute of Cell and Molecular Biology, University of Edinburgh, Scotland.
authors.forename: J
authors.initials: J
authors.lastname: Selfridge
authors.forename: A M
authors.initials: AM
authors.lastname: Pow
authors.forename: J
authors.initials: J
authors.lastname: McWhir
authors.forename: T M
authors.initials: TM
authors.lastname: Magin
authors.forename: D W
authors.initials: DW
authors.lastname: Melton
mesh_terms: D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002678:Chimera; D004260:DNA Repair / Q000235:genetics; D004268:DNA-Binding Proteins; D004720:Endonucleases; D005819:Genetic Markers / Q000235:genetics*; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics*; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D016296:Mutagenesis; D011401:Promoter Regions, Genetic / Q000235:genetics; D014176:Protein Biosynthesis; D011506:Proteins / Q000235:genetics*; D011995:Recombination, Genetic; D012641:Selection, Genetic; D013234:Stem Cells; D014158:Transcription, Genetic
chemical_list: D004268:DNA-Binding Proteins; D005819:Genetic Markers; D011506:Proteins; D007041:Hypoxanthine Phosphoribosyltransferase; D004720:Endonucleases; C499360:Ercc1 protein, mouse