A method for specific analysis of free fatty acids in biological samples by capillary gas chromatography.
The present report describes a simple method to selectively extract free fatty acids and analyze them by capillary gas-liquid chromatography. The procedure is based on the use of fumed silicon dioxide. In the presence of plasma, this material induces a rapid rise in the viscosity of the mixture and presents the ability to trap large particles such as emulsified lipids and lipoproteins. Albumin-bound fatty acids are thus left in the aqueous media. We present applications of our procedure for the analysis of free fatty acids in 0.2 ml of plasma from rat or human. By comparison with the method utilizing thin-layer chromatography for the separation of fatty acids and gas chromatography analysis, the present method has been found to be reliable and simple. The recovery of linoleic acid was 92.1 +/- 8.2%, a value which is about twice better than that obtained with the procedure using thin-layer chromatography. In particular, long-chain polyunsaturated fatty acids were better preserved. Our procedure does not require the use of organic solvents and its simplicity and reproducibility make it suitable for routine specific determination of the composition of free fatty acids in biological samples.
Polette
A
A
INSERM U. 63-22, Bron, France.
Durand
P
P
Floccard
B
B
Blache
D
D
D000818:Animals; D002250:Carbon Radioisotopes; D002849:Chromatography, Gas / Q000379:methods; D002855:Chromatography, Thin Layer / Q000379:methods; D005230:Fatty Acids, Nonesterified / Q000097:blood*; D006801:Humans; D008832:Microchemistry; D011865:Radioisotope Dilution Technique; D051381:Rats
D002250:Carbon Radioisotopes; D005230:Fatty Acids, Nonesterified