An in vitro method for radiolabeling proteins with 35S.
The radiolytic decomposition products of [35S]-methionine have been used to radiolabel proteins in vitro. The process occurs in a time-, temperature-, and pH-dependent manner. Maximum labeling of bovine serum albumin occurs after a 24 h incubation at 37 degrees C and pH 8.5. Once incorporated, the radiolabel cannot be removed by extended incubation at various temperatures, multiple freeze/thaw cycles, or boiling, indicating that the 35S moiety is covalently attached to the protein. A wide variety of proteins have been radiolabeled. The method is simple to perform and yields radiolabeled proteins of high specific activity.
Kalinich
J F
JF
Radiation Biochemistry Department, Armed Forces Radiobiology Research Institute, Bethesda, Maryland 20889-5145.
McClain
D E
DE
D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007553:Isotope Labeling / Q000379:methods*; D008715:Methionine / Q000737:chemistry*; D011506:Proteins; D012710:Serum Albumin, Bovine / Q000737:chemistry; D013462:Sulfur Radioisotopes; D013696:Temperature; D013997:Time Factors
D011506:Proteins; D013462:Sulfur Radioisotopes; D012710:Serum Albumin, Bovine; D008715:Methionine