title: A nuclear magnetic resonance investigation of the upper airways in ferrets. I. Effects of histamine and methacholine.
abstract: Alterations induced in the upper airways of ferrets by intranasal provocation with methacholine (MC) and histamine (HS) were monitored using proton magnetic resonance imaging (MRI) and spin-spin relaxation rate (R2) measurements. Both MC and HS cause a significant increase in the MRI signal intensity and a decrease in R2 in the nasal turbinates. A dose-dependent response is observed for 20 to 315 nmol of HS, with a maximum increase in intensity of ca. 50% occurring above 80 nmol. A single unilateral challenge with MC yields a 62 +/- 3% increase in intensity. Control animals (saline-treated) show little change in image intensity. MC and HS cause decreases in the proton R2 by -27.0 +/- 5.5% and -17.2 +/- 4.3%, respectively. These data are indicative of an accumulation of fluid in the nasal airways. MRI provides an effective means to monitor changes in the nasal airways which occur as a result of pharmacological treatment.
authors.affiliation: Department of Biochemistry, Kettering-Scott Magnetic Resonance Laboratory, Wright State University, Dayton, Ohio.
authors.forename: N V
authors.initials: NV
authors.lastname: Reo
authors.forename: J K
authors.initials: JK
authors.lastname: Barnett
authors.forename: T A
authors.initials: TA
authors.lastname: Neubecker
authors.forename: M E
authors.initials: ME
authors.lastname: Alexander
authors.forename: C M
authors.initials: CM
authors.lastname: Goecke
mesh_terms: D000281:Administration, Intranasal; D000818:Animals; D005289:Ferrets / Q000502:physiology*; D006632:Histamine / Q000008:administration & dosage / Q000494:pharmacology*; D008279:Magnetic Resonance Imaging; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D016210:Methacholine Chloride / Q000008:administration & dosage / Q000494:pharmacology*; D012137:Respiratory System / Q000187:drug effects*; D014420:Turbinates / Q000187:drug effects
chemical_list: D016210:Methacholine Chloride; D006632:Histamine