title: Purification and biochemical properties of calmodulin in Entamoeba histolytica and its distribution during secretion of electron-dense granules.
abstract: 1. Calmodulin (CaM) was detected during secretion of electron-dense granules by Entamoeba histolytica trophozoites with immunofluorescence. 2. It was purified to apparent homogeneity by chromatography with a yield of 2.26 micrograms of calmodulin/mg of protozoan protein. Purity was established by gel electrophoresis. 3. The parasite calmodulin has properties characteristic of calmodulin isolated from other eukaryotes: an apparent molecular weight of 19 or 17 kDa in presence of EGTA or CaCl2, respectively, activation in a calcium dependent manner of bovine heart cyclic nucleotide phosphodiesterase, and its UV spectrum.
authors.affiliation: Department of Genetics and Molecular Biology, Centro de Investigación y de Estudios Avanzados del I.P.N., San Pedro Zacatenco, México, D. F.
authors.forename: M L
authors.initials: ML
authors.lastname: Muńoz
authors.forename: M S
authors.initials: MS
authors.lastname: O'Shea-Alvarez
authors.forename: J
authors.initials: J
authors.lastname: Pérez-García
authors.forename: E C
authors.initials: EC
authors.lastname: Weinbach
authors.forename: M A
authors.initials: MA
authors.lastname: Moreno
authors.forename: M
authors.initials: M
authors.lastname: de la Torre
authors.forename: M A
authors.initials: MA
authors.lastname: Magos
authors.forename: R
authors.initials: R
authors.lastname: Tovar
mesh_terms: D000818:Animals; D002147:Calmodulin / Q000302:isolation & purification / Q000378:metabolism*; D003594:Cytoplasmic Granules / Q000378:metabolism; D004748:Entamoeba histolytica / Q000378:metabolism* / Q000648:ultrastructure; D008856:Microscopy, Fluorescence; D008970:Molecular Weight; D013347:Subcellular Fractions / Q000378:metabolism
chemical_list: D002147:Calmodulin