Structural and functional changes of blood-brain barrier and indication of prion amyloid filaments in experimental amyotrophic leucospongiosis.
The reproduction of amyotrophic leukospongiosis (AL) agent in the central nervous system (CNS) of guinea pigs was accompanied by local disturbance of blood-brain barrier (BBB), which manifested in passing of horseradish peroxidase through the endothelium of some of capillaries (10-14%). The disturbance of BBB function coincided with dystrophic changes in a number of pericapillary astrocyte foot processes and in pericytes. In the walls of altered vessels we observed congophilic (amyloid) deposits, which formed immunocomplexes with monoclonal antibodies to the AL agent protein PrP 27-30 kD. By electron microscopic the deposits consisted of masses of filaments with diameter of 5-10 nm located between the basement membrane and endothelium of the vessels.
Poleshchuk
N N
NN
Byelorussian Research Institute for Epidemiology and Microbiology, Minsk, C.I.S.
Votyakov
V I
VI
Ilkevich YuG
Duboiskaya
G P
GP
Grigoriev
D G
DG
Kolomiets
N D
ND
D000682:Amyloid / Q000378:metabolism*; D000818:Animals; D001812:Blood-Brain Barrier; D001921:Brain / Q000473:pathology*; D001927:Brain Diseases / Q000473:pathology / Q000503:physiopathology*; D006168:Guinea Pigs; D007158:Immunologic Techniques; D017096:Prion Diseases / Q000473:pathology / Q000503:physiopathology*; D011328:Prions / Q000276:immunology / Q000378:metabolism*; D013116:Spinal Cord / Q000473:pathology*
D000682:Amyloid; D011328:Prions