Application of capillary electrophoresis in peptide research.
Comparison of high performance liquid chromatograms (HPLC) with capillary electrophoresis shows the latter to be superior in many cases owing to rapid separation, high resolution and high sensitivity. This is demonstrated with two examples (i) the isolation of natural peptides from bovine tissue and (ii) characterization of a synthetic peptide mixture with the natural sequence (fragment) of the human immunodeficiency virus (HIV) transmembrane glycoprotein gp 41 env.
Yildiz
E
E
Abteilung für Physikalische Biochemie, Physiologisch-chemisches Institut der Universität Tübingen, Germany.
Grübler
G
G
Hörger
S
S
Zimmermann
H
H
Echner
H
H
Stoeva
S
S
Voelter
W
W
D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004586:Electrophoresis / Q000379:methods*; D005069:Evaluation Studies as Topic; D015700:HIV Envelope Protein gp41 / Q000737:chemistry / Q000302:isolation & purification; D006801:Humans; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000302:isolation & purification; D010455:Peptides / Q000737:chemistry / Q000302:isolation & purification*; D013947:Thymosin / Q000737:chemistry / Q000302:isolation & purification
D015700:HIV Envelope Protein gp41; D010446:Peptide Fragments; D010455:Peptides; D013947:Thymosin