[The purification and properties of LD5 from normal C57 mouse liver, H22 hepatoma and S180 sarcoma].
Type-A LDH (LD5), isolated from normal C57 mouse liver, H22 hepatoma and S180 sarcoma after a sequential ion-exchange and affinity chromatography, was examined to be quite pure. Studies on the enzyme kinetics and immunological properties showed that there were no differences in optimal pH, optimal temperature, subunit MW., isoelectric point between normal and malignant (H22 hepatoma and S180 sarcoma) LD5. Km to pyruvate decreased in malignant LD5 while LD5 content and percentage increased. Three LD5 antibodies raised against rabbits showed cross-reaction. These antibodies as probes are useful in the isolation of LD5-mRNAs from G57 mouse liver H22 hepatoma and S180 sarcoma.
Chen
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Huang
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Ning
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D000818:Animals; D000906:Antibodies / Q000032:analysis; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000276:immunology / Q000302:isolation & purification*; D008099:Liver / Q000201:enzymology*; D008114:Liver Neoplasms, Experimental / Q000201:enzymology*; D008297:Male; D051379:Mice; D012510:Sarcoma 180 / Q000201:enzymology*
D000906:Antibodies; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase