The ENZYMEBA test: detection of intestinal Entamoeba histolytica infection by immuno-enzymatic detection of histolysain.
We describe a new, improved test for the detection of intestinal infection by Entamoeba histolytica. The test depends upon immunoadsorption of the E. histolytica cysteine proteinase, histolysain, from faecal samples, and subsequent visual detection of the enzyme by a colour reaction. With samples from 200 volunteers, results agreed closely with those obtained by the conventional microscopic technique, and there were no false positive reactions with samples containing other parasites. The test is suitable for use either in the laboratory or in the field.
Luaces
A L
AL
Departamento de Parasitología, Instituto de Medicina Tropical Pedro Kouri (IPK), Ciudad de la Habana, Cuba.
Picó
T
T
Barrett
A J
AJ
D000563:Amebicides / Q000627:therapeutic use; D000818:Animals; D003429:Cross Reactions; D003546:Cysteine Endopeptidases / Q000032:analysis*; D004404:Dysentery, Amebic / Q000175:diagnosis* / Q000188:drug therapy; D004748:Entamoeba histolytica / Q000201:enzymology*; D005243:Feces / Q000201:enzymology* / Q000469:parasitology; D005663:Furans / Q000627:therapeutic use; D006801:Humans; D007124:Immunoenzyme Techniques; D012680:Sensitivity and Specificity
D000563:Amebicides; D005663:Furans; D003546:Cysteine Endopeptidases; C056423:histolysain; C004932:diloxanide furoate