Increased oxygen species generation in blood monocytes of asthmatic patients.
Besides eosinophils, inflammatory processes in asthma are characterized by an infiltration of inflammatory cells, including mononuclear phagocytes, such as alveolar macrophages (AM) and blood monocytes, in the airways. Monocyte activation has been observed in the blood after exercise or allergen-induced asthma. Stimulated AM in chronic and stable asthmatic patients have been shown to release oxygen species. We thus investigated the intensity of the activation of monocytes from 18 asthmatic patients compared with 18 healthy subjects. Oxygen species release was analyzed for monocytes in suspension by chemiluminescence using a luminometer and for monocytes maintained in adherence using conventional assay and video imaging camera. Circulating blood monocytes in suspension from asthmatic patients and control subjects showed the same baseline free radical release. Monocytes in suspension from asthmatic patients were more stimulatable by PMA: specifically, monocytes release more H2O and peaks of O2-. are sooner; moreover, peaks of total free radical release are higher, and this plateau is sustained. Compared with monocytes from control subjects, those from asthmatic patients evaluated after adherence show a higher baseline for O2-. and higher total free radical release. Monocytes from asthmatic patients spontaneously release more O2-. over time in nonstimulated cells and release more O2-. with PMA stimulation; they show the same peak level total free radical release as those from control subjects after stimulation. SOD activity analysis on adherent monocytes was lower in asthmatic compared with control subjects. These data show that monocytes from asthmatic patients were activated compared with control monocytes.(ABSTRACT TRUNCATED AT 250 WORDS)
Vachier
I
I
INSERM Unité 58, Montpellier, France.
Damon
M
M
Le Doucen
C
C
de Paulet
A C
AC
Chanez
P
P
Michel
F B
FB
Godard
P
P
D000166:Acridines; D000293:Adolescent; D000328:Adult; D001249:Asthma / Q000097:blood* / Q000175:diagnosis / Q000378:metabolism; D002908:Chronic Disease; D005069:Evaluation Studies as Topic; D005260:Female; D005541:Forced Expiratory Volume; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D008163:Luminescent Measurements; D008165:Luminol; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000737:chemistry*; D017382:Reactive Oxygen Species / Q000737:chemistry*; D012720:Severity of Illness Index; D013482:Superoxide Dismutase / Q000378:metabolism
D000166:Acridines; D017382:Reactive Oxygen Species; C033472:10,10'-dimethyl-9,9'-biacridinium; D008165:Luminol; D006861:Hydrogen Peroxide; D013482:Superoxide Dismutase