A specific peroxidase-coupled activity stain for diamine oxidases.
A specific peroxidase-coupled activity staining method for diamine oxidase (DAO) was developed. Diaminobenzidine was found to inhibit DAO and to give rise to unspecific staining. Among several other reagents 4-Cl-1-naphthol was found to be most suitable. Using specific activity staining DAO could be visualized in polyacrylamide gels as a high-molecular-weight complex, which could be dissociated by Tween 20 but not by NP-40, Triton X-100, or Chaps.
Houen
G
G
Institute of Biochemical Genetics, Copenhagen, Denmark.
Leonardsen
L
L
D015100:3,3'-Diaminobenzidine / Q000494:pharmacology; D006631:Amine Oxidase (Copper-Containing) / Q000032:analysis* / Q000037:antagonists & inhibitors / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D066298:In Vitro Techniques; D008970:Molecular Weight; D009284:Naphthols / Q000494:pharmacology*; D010084:Oxidation-Reduction; D010544:Peroxidases / Q000378:metabolism*; D013379:Substrate Specificity
D009284:Naphthols; D015100:3,3'-Diaminobenzidine; C025917:4-chloro-1-naphthol; D010544:Peroxidases; D006631:Amine Oxidase (Copper-Containing)