Effects of bile salt exposure on pancreatic duct barrier function and protein release determined by two-dimensional electrophoresis and lectin-affinoblotting.
We analyzed effects of a bile salt on pancreatic duct barrier function and protein release into pancreatic juice in cats, using two-dimensional-electrophoresis and lectin-affinoblotting. Preanalyzed pancreatic juice was perfused along the pancreatic duct following duct exposure to a bile salt. In this case, pancreatic duct mucosa showed a partial loss of barrier function to the leakage of exocrine pancreatic proteins dependent upon molecular weight and charge. The release of a sialic acid possessing protein into pancreatic juice was observed.
Hänsler
M
M
Institute of Policlinical Medicine, Leipzig University, Germany.
Arendt
T
T
Lange
K
K
D000208:Acute Disease; D000818:Animals; D001647:Bile Acids and Salts / Q000494:pharmacology*; D002415:Cats; D015180:Electrophoresis, Gel, Two-Dimensional; D006023:Glycoproteins / Q000302:isolation & purification / Q000378:metabolism*; D037102:Lectins; D010183:Pancreatic Ducts / Q000187:drug effects* / Q000378:metabolism; D010189:Pancreatic Juice / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D010195:Pancreatitis / Q000209:etiology; D010539:Permeability / Q000187:drug effects; D051381:Rats
D001647:Bile Acids and Salts; D006023:Glycoproteins; D037102:Lectins