On the mechanism of rat uterus desensitization to kallikrein.
An inactive form of kallikrein prepared by iodination with cold iodine, did not show any enzymatic or oxytocic action. However, a competitive pattern between this inactive and active kallikrein was observed in rat uterus preparation: When the inactive form was applied several times in the muscle, a single dose of active kallikrein was unable to cause contraction, but a double dose elicited a response. The rhythmic movement caused by a singular dose of active kallikrein, had its time curtailed by adding the inactive kallikrein to the bath. The inactive kallikrein did not interfere with bradykinin activity.
Heneine
I F
IF
Biophysics Laboratory, Federal University of Minas Gerais, Belo Horizonte, Brazil.
Feitosa
M H
MH
Beraldo
W T
WT
Oliveira
G M
GM
Pesquero
J L
JL
D000818:Animals; D037263:Asialoglycoprotein Receptor; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D066298:In Vitro Techniques; D007610:Kallikreins / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D010052:Ovariectomy; D051381:Rats; D011971:Receptors, Immunologic / Q000187:drug effects / Q000502:physiology; D014590:Uterine Contraction / Q000187:drug effects*; D014599:Uterus / Q000187:drug effects / Q000502:physiology*
D037263:Asialoglycoprotein Receptor; D011971:Receptors, Immunologic; D004958:Estradiol; D007610:Kallikreins