title: [Pepsin in the enzymatic synthesis of esters and n-nitroanilide peptides].
abstract: Pepsin was shown to catalyze synthesis of esters or p-nitroanilides tri-, tetra-, penta- and hexapeptides of general formula Z-X-Y-B, where X = Ala-Phe, Phe-Met, Ala-Ala-Glu, Ala-Ala-Phe, Ala-Ala-Leu, Ala-Ala-Trp, Ala-Ala-Met. Y = Ala, Leu, Val, Phe, Arg, Ala-Ala, Gly-Gly, Leu-Ala-Ala, Phe-Ala-Ala. B = OMe, pNA. The reactions were carried out in dimethylformamide-water solutions at pH 4.6 by equimolar ratio of amino- and carboxyl components (with the exception of Arg-pNA taken in 2-fold excess). The amount of pepsin in the reaction approached 1:1700 enzyme: substrate molar ratio although it might be improved--up to 1:3.10(5) for relatively long peptides.
authors.forename: E N
authors.initials: EN
authors.lastname: Lysogorskaia
authors.forename: I Iu
authors.initials: IIu
authors.lastname: Filippova
authors.forename: K A
authors.initials: KA
authors.lastname: Abdel' Malak
authors.forename: G I
authors.initials: GI
authors.lastname: Lavrenova
authors.forename: V V
authors.initials: VV
authors.lastname: Anisimova
authors.forename: E S
authors.initials: ES
authors.lastname: Oksenoĭt
authors.forename: V M
authors.initials: VM
authors.lastname: Stepanov
mesh_terms: D000595:Amino Acid Sequence; D000813:Anilides / Q000737:chemistry*; D000818:Animals; D002384:Catalysis; D004952:Esters / Q000737:chemistry; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000138:chemical synthesis*; D010434:Pepsin A / Q000737:chemistry*; D010455:Peptides / Q000138:chemical synthesis*; D013552:Swine
chemical_list: D000813:Anilides; D004952:Esters; D009842:Oligopeptides; D010455:Peptides; D010434:Pepsin A
vernacular_title: Pepsin v fermentativnom sintexze éfirov i n-nitroanilidov peptidov.