Investigation on glutamine amidohydrolase (EC 3.5.1.2) and glutamine aminotransferase (EC 2.5.1.15) activity in liver and plasma of EAC-bearing mice following glutaminase therapy.
The anti-neoplastic activity of bacterial glutaminase on Ehrlich ascites tumor-bearing mice was studied by determining the reduction in the tumor cell count and extension of life span of the host after therapy. The therapeutic effect of glutaminase in relation to change in activity of glutaminolytic enzymes (glutamine amidohydrolase (GNase) and glutamine aminotransferase (GAt)) in liver and plasma were also studied. Bacterial glutaminase was shown to be effective in lowering the tumor burden with increased life span of the host. Glutamine amidohydrolase activity in the liver and plasma was raised significantly with increased tumor burden, whereas GAt activity remained unchanged. Following glutaminase therapy, this high level of GNase activity decreased in comparison to the untreated control. These changes were not seen when normal mice were treated with the same enzyme. Thus alteration in the enzyme levels, particularly GNase was observed to have some correlation with progression of the tumor growth.
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Department of Cell Biology, Chittaranjan National Cancer Institute, Calcutta, India.
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D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000097:blood / Q000188:drug therapy* / Q000201:enzymology; D005972:Glutaminase / Q000627:therapeutic use*; D008099:Liver / Q000201:enzymology*; D051379:Mice; D013997:Time Factors; D000637:Transaminases / Q000097:blood / Q000378:metabolism*
D000637:Transaminases; C020656:glutamine-pyruvate aminotransferase; D005972:Glutaminase