title: Long-term biodistribution in tumored mice of murine and chimeric B72.3-IgG antibody radiolabeled with 114mIn via both DTPA and a macrocyclic chelator.
abstract: To assess the possibilities of using 114mIn as a therapeutic agent, the long-term biodistribution of 114mIn was studied, in tumor-bearing nude mice, after injection of labeled monoclonal antibody (MoAb) B72.3 IgG, either DTPA-coupled murine, DTPA-coupled chimeric, or macrocycle-coupled chimeric antibody. Although the biodistributions in all cases were similar, there were important differences. The use of DTPA-coupled chimeric antibody led to higher concentrations of radioactivity in tumor, and to lower concentrations in liver and bone, as compared to DTPA-coupled murine antibody. The use of macrocycle-coupled chimeric antibody led to higher concentrations of radioactivity in the liver and in bone as compared to the DTPA-coupled chimeric antibody. However, in this case there were no significant differences in tumor uptake or clearance. Radiation doses were calculated based on the organ retention and by neglecting source-to-target contributions. Radiation dose distribution was marginally favorable for therapy in the group injected with DTPA-coupled chimeric antibody.
authors.affiliation: Department of Nuclear Medicine, University of Massachusetts Medical Center, Worcester 01655.
authors.forename: M
authors.initials: M
authors.lastname: Aas
authors.forename: G
authors.initials: G
authors.lastname: Mardirossian
authors.forename: T W
authors.initials: TW
authors.lastname: Griffin
authors.forename: A R
authors.initials: AR
authors.lastname: Salimi
authors.forename: T
authors.initials: T
authors.lastname: Ito
authors.forename: H S
authors.initials: HS
authors.lastname: Bushe
authors.forename: R W
authors.initials: RW
authors.lastname: Atcher
authors.forename: J J
authors.initials: JJ
authors.lastname: Hines
authors.forename: A B
authors.initials: AB
authors.lastname: Brill
authors.forename: D J
authors.initials: DJ
authors.lastname: Hnatowich
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000008:administration & dosage; D001345:Autoradiography; D002460:Cell Line; D002614:Chelating Agents; D004307:Dose-Response Relationship, Radiation; D005260:Female; D007205:Indium Radioisotopes / Q000008:administration & dosage / Q000493:pharmacokinetics*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009374:Neoplasms, Experimental / Q000378:metabolism* / Q000532:radiotherapy; D004369:Pentetic Acid; D014018:Tissue Distribution
chemical_list: D000911:Antibodies, Monoclonal; D002614:Chelating Agents; D007205:Indium Radioisotopes; D004369:Pentetic Acid