Chromogranin A processing in sympathetic neurons and release of chromogranin A fragments from sheep spleen.
Chromogranin A (CGA) has been localized to the large dense cored vesicles (LDV) of sympathetic neurons. SDS-PAGE and immunoblotting of soluble LDV proteins from ox and dog adrenergic neuronal cell bodies, axons and nerve terminals, revealed an increasing number of CGA-immunoreactive forms, consistent with proteolytic processing during axonal transport. Splenic nerve electrical stimulation (10 Hz, 2 min) revealed that, apart from CGA, these CGA-processing products are released from the sheep spleen. The secretion of CGA-derived fragments from sympathetic neurons might suggest a role in the regulation of synaptic transmission.
Miserez
B
B
Department of Medicine, University of Antwerp (UIA), Wilrijk, Belgium.
Annaert
W
W
Dillen
L
L
Aunis
D
D
De Potter
W
W
D000320:Adrenergic Fibers / Q000378:metabolism*; D000818:Animals; D001692:Biological Transport; D015153:Blotting, Western; D002417:Cattle; D053379:Chromogranin A; D002864:Chromogranins / Q000302:isolation & purification / Q000378:metabolism*; D004285:Dogs; D011499:Protein Processing, Post-Translational; D012756:Sheep; D013154:Spleen / Q000294:innervation / Q000378:metabolism*; D013347:Subcellular Fractions
D053379:Chromogranin A; D002864:Chromogranins