title: Serum lactate dehydrogenase isoenzyme 1 activity in patients with testicular germ cell tumors correlates with the total number of copies of the short arm of chromosome 12 in the tumor.
abstract: The aim of our study was to assess the relationship between the serum lactate dehydrogenase isoenzyme 1 (S-LDH-1) activity in patients with testicular germ cell tumors and the number of copies of the short arm of chromosome 12 (12p) present in tumor. Twenty-seven adult patients with measurable tumor lesions were studied. Twenty-five had three or more copies of chromosome 12 per cell in the tumors. Nineteen had one or more copies of a specific chromosomal abnormality, an isochromosome of the short arm of chromosome 12, i(12p). Fourteen had increased S-LDH-1 levels. S-LDH-1 activity correlated significantly with the product of total tumor volume and the total number of copies of the short arm of chromosome 12 present per cell (total tumor 12p). We conclude that the total number of copies of the short arm of chromosome 12 in the tumors is most probably a factor contributing to the LDH-1 activity released from the tumors.
authors.affiliation: Department of Clinical Chemistry, Odense University Hospital, Denmark.
authors.forename: F E
authors.initials: FE
authors.lastname: von Eyben
authors.forename: W E
authors.initials: WE
authors.lastname: de Graaff
authors.forename: J
authors.initials: J
authors.lastname: Marrink
authors.forename: O
authors.initials: O
authors.lastname: Blaabjerg
authors.forename: D T
authors.initials: DT
authors.lastname: Sleijfer
authors.forename: H S
authors.initials: HS
authors.lastname: Koops
authors.forename: J W
authors.initials: JW
authors.lastname: Oosterhuis
authors.forename: P H
authors.initials: PH
authors.lastname: Petersen
authors.forename: J
authors.initials: J
authors.lastname: van Echten-Arends
authors.forename: B
authors.initials: B
authors.lastname: de Jong
mesh_terms: D000328:Adult; D002869:Chromosome Aberrations; D002881:Chromosomes, Human, Pair 12; D006801:Humans; D007527:Isoenzymes; D007621:Karyotyping; D007770:L-Lactate Dehydrogenase / Q000097:blood* / Q000235:genetics; D008297:Male; D008875:Middle Aged; D013736:Testicular Neoplasms / Q000097:blood / Q000201:enzymology* / Q000235:genetics
chemical_list: D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase