title: Permeability of the normal round window membrane to Haemophilus influenzae type b endotoxin.
abstract: Sensorineural hearing loss associated with otitis media may be due to passage of ototoxic substances such as bacterial toxins and antibiotics, from the middle ear into the inner ear. The round window membrane is the most likely route for such transport. The aim of this study was to analyze the extent of endotoxin passage through the normal round window membrane. The round window membranes of 19 chinchillas were exposed in vivo to Gelfoam soaked in purified Haemophilus influenzae type b endotoxin at a concentration of 45,000 endotoxin units per ml (EU/ml) during 3 to 24 h. Endotoxin levels in the perilymph were measured with Limulus Amaebocyte Lysate or Quantitative Chromogenic Limulus Amaebocyte Lysate. Endotoxin was detected in half of the inner ears at concentrations close to the detection limit (approximately 4 EU/ml). The results suggest that the normal round window membrane efficiently protects the inner ear against the passage of bacterial endotoxins from the middle ear cavity. It is unlikely that endotoxin at concentrations found in the middle ear secretion during otitis media can traverse the round window membrane in sufficient amount to cause inner ear deterioration.
authors.affiliation: Department of Otorhinolaryngology, Karolinska Hospital, Karolinska Institute, Stockholm, Sweden.
authors.forename: L
authors.initials: L
authors.lastname: Lundman
authors.forename: S K
authors.initials: SK
authors.lastname: Juhn
authors.forename: D
authors.initials: D
authors.lastname: Bagger-Sjöbäck
authors.forename: C
authors.initials: C
authors.lastname: Svanborg
mesh_terms: D000818:Animals; D002682:Chinchilla; D007758:Ear, Inner / Q000378:metabolism; D004731:Endotoxins / Q000032:analysis / Q000493:pharmacokinetics*; D006193:Haemophilus influenzae / Q000737:chemistry; D008070:Lipopolysaccharides / Q000032:analysis / Q000378:metabolism; D008566:Membranes / Q000378:metabolism; D010498:Perilymph / Q000737:chemistry / Q000378:metabolism; D010539:Permeability; D012405:Round Window, Ear / Q000378:metabolism*; D013997:Time Factors
chemical_list: D004731:Endotoxins; D008070:Lipopolysaccharides