Automated rate nephelometric determination of apolipoproteins AI and B in human serum by consecutive addition of antibodies.
A precise, rapid, automated, rate nephelometric immunoassay for apolipoprotein AI (APA) and apolipoprotein B (APB) is described. Both analytes are determined by a "one-pot" procedure which uses consecutive addition of the corresponding antibodies. Poly(oxyethylene) type nonionic surfactants are used to selectively inhibit the APB reaction after the maximum reaction rate has been reached and to enhance the APA immunoreactivity. The assay range for APA is 0.3-3.8 g/L and for APB 0.2-3.0 g/L. The new assay was compared to commercial rate nephelometric methodology. Both methods were shown to be mutually unbiased for APA and APB determinations in human serum.
Fotiou
F K
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Sanofi Diagnostics Pasteur, Inc., Immunology Research and Development, Chaska, Minnesota 55318.
D000906:Antibodies / Q000032:analysis; D016632:Apolipoprotein A-I / Q000032:analysis* / Q000276:immunology; D001055:Apolipoproteins B / Q000097:blood* / Q000276:immunology; D006801:Humans; D007118:Immunoassay; D007202:Indicators and Reagents
D000906:Antibodies; D016632:Apolipoprotein A-I; D001055:Apolipoproteins B; D007202:Indicators and Reagents