title: [Patch-clamp technique].
abstract: We first describe how to set up the system for patch-clamp recording and carry out the experiments with 4 different modes of conventional patch-clamp technique: cell-attached, whole-cell, inside-out and outside-out configurations. Thereafter, we mention about an improved 'nystatin perforated' patch-clamp technique which dissolves the fault of conventional patch-clamp techniques except the cell-attached mode.
authors.affiliation: Department of Neurophysiology, Tohoku University School of Medicine, Sendai, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Akaike
authors.forename: T
authors.initials: T
authors.lastname: Shirasaki
mesh_terms: D000818:Animals; D004594:Electrophysiology / Q000295:instrumentation / Q000379:methods*; D008564:Membrane Potentials; D009474:Neurons / Q000502:physiology