Tissue distribution of insulin-like growth factor receptors in the turkey.
1. The distribution and relative insulin-like growth factor (IGF) binding capacities of membranes derived from 14 tissues of the turkey were examined. 2. Affinity cross-linking analyses using [125I]IGF-I and [125I]IGF-II with membranes derived from the liver, pectoralis major muscle, gizzard, heart and brain indicated that both IGFs interact with only type-I IGF receptors on these tissues. 3. There was no evidence for the existence of a type-II IGF receptor in any tissue. 4. Although considerable variation was detected in the molecular weights of the IGF receptor alpha subunits between tissues (112.2-132.9 kDa), these differences did not appear to influence receptor-ligand affinities.
McFarland
D C
DC
Department of Animal and Range Sciences, South Dakota State University, Brookings 57007-0392.
Ferrin
N H
NH
Gilkerson
K K
KK
Pesall
J E
JE
D000818:Animals; D001667:Binding, Competitive; D066298:In Vitro Techniques; D007700:Kinetics; D008297:Male; D008566:Membranes / Q000378:metabolism; D008970:Molecular Weight; D011487:Protein Conformation; D017526:Receptor, IGF Type 1 / Q000737:chemistry / Q000378:metabolism*; D017527:Receptor, IGF Type 2 / Q000378:metabolism; D014018:Tissue Distribution; D014422:Turkeys / Q000378:metabolism*
D017527:Receptor, IGF Type 2; D017526:Receptor, IGF Type 1