title: Rapid quality control of 99Tcm-sestamibi.
abstract: The thin layer chromatographic (TLC) method for determining the radiochemical purity of 99Tcm-sestamibi suggested by the manufacturer of the kit is slow and inconvenient. In this study a more rapid reversed phase chromatography (Sep-Pak) technique is compared with the TLC method for measurement of the radiochemical purity of 99Tcm-sestamibi. The levels of free 99Tcm-pertechnetate in 99Tcm-sestamibi were accurately determined using the Sep-Pak technique. However, due to binding of variable amounts of reduced-hydrolysed unbound 99Tcm to the chromatography cartridge, the Sep-Pak method overestimated the radiochemical purity of the radiopharmaceutical by approximately 3%. In practice, this overestimation is not important and the Sep-Pak technique can be used as a rapid method of determining radiochemical purity of 99Tcm-sestamibi.
authors.affiliation: Division of Nuclear Medicine, Toronto Hospital, Ontario, Canada.
authors.forename: R M
authors.initials: RM
authors.lastname: Reilly
authors.forename: M
authors.initials: M
authors.lastname: So
authors.forename: J
authors.initials: J
authors.lastname: Polihronis
authors.forename: S
authors.initials: S
authors.lastname: Houle
mesh_terms: D011786:Quality Control; D017256:Technetium Tc 99m Sestamibi / Q000592:standards*; D013997:Time Factors
chemical_list: D017256:Technetium Tc 99m Sestamibi