title: Hybrid artificial pancreas: islet transplantation inside membrane bioreactors.
abstract: The use of pancreatic islet transplantation in membrane bioreactors put in vascular circuits aims at resetting the glucose homeostasis in diabetic or pancreatectomized patients, avoiding immune host rejection. Our experience was carried out at following stages: porcine pancreas explantation and enzymatic separation of endocrine tissue from exocrine fraction by collagenase; evaluation of islet functionality (culture tests); in vitro tests of the islets-bioreactor system, to assess the metabolic response to the glucose; in vivo evaluation to assay the haemodynamic behaviour. The trials showed a good metabolic bioreactor functionality and a decreasing incidence of coagulative problems.
authors.affiliation: Chair of Surgical Pathology, Catholic University, Rome, Italy.
authors.forename: C P
authors.initials: CP
authors.lastname: Lombardi
authors.forename: A
authors.initials: A
authors.lastname: Urso
authors.forename: G
authors.initials: G
authors.lastname: Careddu
authors.forename: G
authors.initials: G
authors.lastname: Ghirlanda
authors.forename: G
authors.initials: G
authors.lastname: Catapano
authors.forename: G
authors.initials: G
authors.lastname: Brisinda
authors.forename: F
authors.initials: F
authors.lastname: Ceriati
authors.forename: R
authors.initials: R
authors.lastname: Bellantone
authors.forename: G B
authors.initials: GB
authors.lastname: Doglietto
authors.forename: F
authors.initials: F
authors.lastname: Crucitti
mesh_terms: D000818:Animals; D005260:Female; D005947:Glucose / Q000378:metabolism; D006493:Heparin; D007328:Insulin / Q000378:metabolism; D000078790:Insulin Secretion; D016381:Islets of Langerhans Transplantation / Q000379:methods*; D008567:Membranes, Artificial; D013552:Swine
chemical_list: D007328:Insulin; D008567:Membranes, Artificial; D006493:Heparin; D005947:Glucose