Determination of proteinase 3-alpha 1-antitrypsin complexes in inflammatory fluids.
Physiological inhibitors were tested for their in vitro interaction with neutrophil proteinase 3 (PR3). The major plasma proteinase inhibitor of PR3 is alpha 1AT. We have developed a radioimmunoassay (RIA) for quantitative detection of PR3-alpha 1AT complexes formed in vivo in inflammatory exudates such as synovial fluid and plasma from patients with sepsis. Levels of PR3-alpha 1AT complexes correlated significantly with levels of human neutrophil elastase (HNE)-alpha 1AT complexes. Thus, in vivo alpha 1AT not only protects against excessive HNE activity, but also against excessive PR3 activity.
Dolman
K M
KM
Central Laboratory The Netherlands Red Cross Blood Transfusion Service Amsterdam, Amsterdam.
van de Wiel
B A
BA
Kam
C M
CM
Abbink
J J
JJ
Hack
C E
CE
Sonnenberg
A
A
Powers
J C
JC
von dem Borne
A E
AE
Goldschmeding
R
R
D001172:Arthritis, Rheumatoid / Q000378:metabolism; D016470:Bacteremia / Q000378:metabolism; D006801:Humans; D007249:Inflammation / Q000378:metabolism*; D053146:Myeloblastin; D011487:Protein Conformation; D011863:Radioimmunoassay / Q000379:methods; D012697:Serine Endopeptidases / Q000097:blood / Q000378:metabolism*; D000515:alpha 1-Antitrypsin / Q000032:analysis / Q000378:metabolism*
D000515:alpha 1-Antitrypsin; D012697:Serine Endopeptidases; D053146:Myeloblastin