title: Oestrogen receptor protein and mRNA in adenocarcinoma of the uterine cervix.
abstract: We have investigated the oestrogen receptor (ER) status of 20 cervical adenocarcinomas by immunocytochemistry for ER protein and non-isotopic in situ hybridisation for ER mRNA. Both methods, which are applicable to paraffin sections, were developed and validated in breast carcinomas with known ER content. Six cervical adenocarcinomas contained immunocytochemically demonstrable ER protein; all contained ER mRNA, but staining was less intense in poorly differentiated areas of four tumours. This disparity between protein and mRNA detection needs further investigation as does the possibility that oestrogens may play a role in the pathogenesis of cervical adenocarcinoma.
authors.affiliation: Department of Pathology, University of Wales College of Medicine, Heath Park, Cardiff, UK.
authors.forename: S M
authors.initials: SM
authors.lastname: Ismail
authors.forename: G A
authors.initials: GA
authors.lastname: Thomas
authors.forename: F A
authors.initials: FA
authors.lastname: Ghandour
authors.forename: H G
authors.initials: HG
authors.lastname: Davies
authors.forename: R
authors.initials: R
authors.lastname: Attanoos
authors.forename: E D
authors.initials: ED
authors.lastname: Williams
mesh_terms: D000230:Adenocarcinoma / Q000737:chemistry*; D000595:Amino Acid Sequence; D001943:Breast Neoplasms / Q000737:chemistry; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000032:analysis*; D012334:RNA, Neoplasm / Q000032:analysis*; D011960:Receptors, Estrogen / Q000032:analysis*; D002583:Uterine Cervical Neoplasms / Q000737:chemistry*
chemical_list: D012333:RNA, Messenger; D012334:RNA, Neoplasm; D011960:Receptors, Estrogen