[The use of immunoenzyme analysis for determining ABH antigens in traces of saliva and sperm].
The authors suggest a simple sensitive technique for enzyme immunoassay (EIA) of ABH antigens in saliva and semen. A two-staged dot blot solid-phase EIA on nitrocellulose membranes was employed with anti-ABH monoclonal antibodies obtained in immunization of mice with human red cells. 4-chloro-1-naphthol substrate solution was used to visualize the peroxidase label. The results of analysis of salivary and spermatic samples obtained from donors of various groups evidence that this EIA variant may be useful in forensic medicine.
Deriugina
E I
EI
Savel'ev
Iu I
IuI
Nosyrev
A E
AE
Lapenkov
M I
MI
Nikolaeva
T L
TL
D000017:ABO Blood-Group System / Q000276:immunology*; D000911:Antibodies, Monoclonal; D000941:Antigens / Q000032:analysis*; D002021:Buffers; D006801:Humans; D015151:Immunoblotting / Q000379:methods; D007124:Immunoenzyme Techniques; D007202:Indicators and Reagents; D007983:Lewis Blood Group Antigens / Q000276:immunology; D008297:Male; D012463:Saliva / Q000737:chemistry* / Q000276:immunology; D013094:Spermatozoa / Q000737:chemistry* / Q000276:immunology; D013997:Time Factors
D000017:ABO Blood-Group System; D000911:Antibodies, Monoclonal; D000941:Antigens; D002021:Buffers; D007202:Indicators and Reagents; D007983:Lewis Blood Group Antigens
Primenenie immunofermentnogo analiza dlia opredeleniia ABH-antigenov v sledakh sliuny i spermy.