Poly(ethylene oxide)-graft-poly(L-lysine) copolymers to enhance the biocompatibility of poly(L-lysine)-alginate microcapsule membranes.
A graft copolymer having poly(L-lysine) (PLL) as the backbone and monomethoxy poly(ethylene glycol) (MPEG) as pendent chains was synthesized. This polycationic copolymer was used to form microcapsules with sodium alginate, a polyanion. Microcapsules and model surfaces formed with PLL-graft-MPEG demonstrated reduced protein adsorption, complement binding and cell adhesion in vitro compared to materials with unmodified PLL. Microcapsules with PLL-g-MPEG on the surface were seen to be much more biocompatible than the widely used alginate/PLL/alginate microcapsule in a mouse intraperitoneal implant model. The graft copolymers demonstrated a lower affinity for alginate and increased microcapsule permeability more than PLL. To correct this, pentalayered alginate/PLL/alginate/PLL-g-MPEG/alginate microcapsules were fabricated, and these demonstrated both appropriate permselectivity and enhanced biocompatibility.
Sawhney
A S
AS
Department of Chemical Engineering, University of Texas, Austin 78712-1062.
Hubbell
J A
JA
D000327:Adsorption; D000464:Alginates / Q000737:chemistry*; D000818:Animals; D001672:Biocompatible Materials; D002448:Cell Adhesion; D003165:Complement System Proteins / Q000378:metabolism; D005340:Fibrinogen / Q000737:chemistry; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D066298:In Vitro Techniques; D008567:Membranes, Artificial; D051379:Mice; D008854:Microscopy, Electron; D010537:Peritoneum / Q000166:cytology; D010539:Permeability; D011092:Polyethylene Glycols / Q000737:chemistry*; D011107:Polylysine / Q000737:chemistry*; D011108:Polymers; D019736:Prostheses and Implants
D000464:Alginates; D001672:Biocompatible Materials; D006603:Hexuronic Acids; D008567:Membranes, Artificial; D011108:Polymers; D011107:Polylysine; D011092:Polyethylene Glycols; D020723:Glucuronic Acid; D005340:Fibrinogen; D003165:Complement System Proteins