[Monoclonal antibodies to the influenza A virus matrix protein that react with viral strains of different subtypes].
To obtain hybridomas secreting monoclonal antibodies (MCA) to matrix (M) protein of influenza virus, mice were immunized with a modified antigen which consisted of subvirus units after electrophoretic removal of surface glycoproteins from virions using Desintegnon-O detergent. Six stable hybrid cultures producing MCA to M-protein were derived. The properties of MCA to the antigen determinant common for a group of influenza A virus strains of different subtypes allow them to be used in the development of a test-system for diagnosis of influenza A type.
Popov
I A
IA
Chervonskiĭ
A V
AV
Krivoshein
Iu S
IuS
Krivorutchenko
Iu L
IuL
Bakova
A A
AA
Fedorkina
O A
OA
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000276:immunology* / Q000302:isolation & purification; D000914:Antibodies, Viral / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification; D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions; D000956:Antigens, Viral / Q000276:immunology / Q000302:isolation & purification; D005260:Female; D006825:Hybridomas / Q000276:immunology; D007114:Immunization; D015151:Immunoblotting; D007124:Immunoenzyme Techniques; D007132:Immunoglobulin Isotypes / Q000032:analysis; D009980:Influenza A virus / Q000276:immunology*; D009981:Influenza B virus / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D014763:Viral Matrix Proteins / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D007132:Immunoglobulin Isotypes; D014763:Viral Matrix Proteins
Monoklonal'nye antitela k matriksnomu belku virusa grippa A, reagiruiushchie s virusnymi shtammami razlichnykh podtipov.