title: In vitro production of specific antibody by equine peripheral blood mononuclear cells using tetanus toxoid as a recall antigen.
abstract: Anti-tetanus toxoid (TT) antibody (Ig) levels in the supernatant of cultured, pre-immunised equine peripheral blood mononuclear cells (PBMC) were measured by an indirect enzyme-linked immunoabsorbent assay (ELISA). Optimal anti-TT Ig production occurred at concentrations of stimulating, purified TT of between 0.001 and 0.1 micrograms ml-1, which varied depending on the cell concentration. Optimal anti-TT Ig production was most consistently produced when the cell concentration was 5 x 10(6) ml-1. At this cell concentration maximal anti-TT Ig was induced using 0.1 micrograms ml-1 TT. At a cell concentration of 5 x 10(6) ml-1 and a TT concentration of 0.1 micrograms ml-1 anti-TT Ig was first detectable in supernatant on day 5 of stimulation. Maximal levels of anti-TT Ig were present in the supernatant by day 10. No anti-TT Ig was produced in cultures of PBMC from non-immune animals.
authors.affiliation: University of Cambridge, Department of Clinical Veterinary Medicine, Cambridge.
authors.forename: M A
authors.initials: MA
authors.lastname: O'Brien
authors.forename: M A
authors.initials: MA
authors.lastname: Holmes
authors.forename: W P
authors.initials: WP
authors.lastname: Duffus
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis* / Q000276:immunology; D000918:Antibody Specificity; D002478:Cells, Cultured; D006736:Horses / Q000276:immunology*; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D013745:Tetanus Toxoid / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D013745:Tetanus Toxoid