Anti-influenza virus activity of a lignin fraction from cone of Pinus parviflora Sieb. et Zucc.
When mice were inoculated intranasally or intracerebrally with lethal doses of influenza virus A/WSN/33, most died within 12 days. However, the infectivity of virus that had been preincubated with a lignin prepared from cones of Pinus parviflora Sieb. et Zucc. (PC-Fr. VI) was significantly reduced. Intraperitoneal or oral administration of PC-Fr. VI, prior to virus inoculation, slightly increased the survival ratio of the infected mice. Experiments using radiolabeled PC-Fr. VI revealed that this fraction effectively binds to virions as well as to cultured cells. These data suggest that PC-Fr. VI either inactivates the virus or induces the anti-viral state in cells by binding to virions or cells.
Sakagami
H
H
First Department of Biochemistry, School of Medicine, Showa University, Tokyo, Japan.
Takeda
M
M
Kawazoe
Y
Y
Nagata
K
K
Ishihama
A
A
Ueda
M
M
Yamazaki
S
S
D000284:Administration, Oral; D000818:Animals; D000998:Antiviral Agents / Q000008:administration & dosage / Q000302:isolation & purification* / Q000494:pharmacology; D002460:Cell Line; D004285:Dogs; D006801:Humans; D009980:Influenza A virus / Q000187:drug effects*; D007274:Injections, Intraperitoneal; D008031:Lignin / Q000008:administration & dosage / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D014197:Trees; D014771:Virion / Q000187:drug effects
D000998:Antiviral Agents; D008031:Lignin