title: Variants of the mucosal mast cell line (RBL-2H3) deficient in a functional membrane glycoprotein.
abstract: We have isolated and characterized subpopulations of the rat mucosal mast cell line, RBL-2H3, carrying either high or low density of a glycoprotein, recently established as mast cell function-associated antigen (MAFA, Ortega et al., 1991), on their surface. These populations were investigated in order to better define the involvement of the MAFA in coupling the immunological stimulation of mast cells to mediator release. The MAFA density on the cell surface of the deficient subpopulation was less than or equal to 10-20% that of the parental population and this phenotype was found to be stably maintained for several months. In contrast, the MAFA-enriched cells had maximally twice the number of copies per cell surface than that of the parental population and this phenotype was less stable. Significantly, low copy number of MAFA on the cell's surface was accompanied by a markedly different secretory response, i.e. (i) a considerable decrease in the secretory response to the Fc epsilon RI-mediated stimulus (ii) a marked enhancement of the ionomycin induced secretion. In order to gain insight into the causes for this decrease in cellular response to the Fc epsilon RI-mediated stimulus, we measured the amplitudes of several biochemical processes which are assigned to the stimulus-secretion coupling cascade. The Fc epsilon RI-mediated uptake of 45Ca2+ by the MAFA-deficient cells was considerably lower than that of the parental and MAFA-enriched cells. Similarly, these cell's Fc epsilon RI-induced rise in [Ca2+]i (both the initial transient as well as the sustained elevation), was markedly lower than that of the parental line and the MAFA-enriched cells. Moreover, the low initial transient rise in [Ca2+]i was found to be correlated with the decrease in Fc epsilon RI-mediated IP3 levels. We therefore examined the cell's content of the phosphatidyl-inositides hydrolyzing enzyme, phospholipase C gamma 1. This was found to be similar in the parental line and in its derived subpopulations. However, PLC gamma 1 activation, as measured by the time course of phosphorylation of its tyrosines, showed a marked difference: while PLC gamma 1 tyrosine phosphorylation, in the parental cells, was only transient (detected already 1 min after antigen addition and declined afterwards to basal levels at ca. 10 min), in the MAFA-deficient cells, tyrosine phosphorylated PLC gamma 1 was also observed 1 min after antigen addition, yet showed no decrease with time in its phosphorylation intensity for up to 30 min.(ABSTRACT TRUNCATED AT 400 WORDS)
authors.affiliation: Department of Chemical Immunology, Weizmann Institute of Science, Rehovot, Israel.
authors.forename: A
authors.initials: A
authors.lastname: Cohen-Dayag
authors.forename: H
authors.initials: H
authors.lastname: Schneider
authors.forename: I
authors.initials: I
authors.lastname: Pecht
mesh_terms: D000814:Aniline Compounds; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002132:Calcium Radioisotopes / Q000378:metabolism; D002452:Cell Count; D002453:Cell Cycle; D002460:Cell Line; D002469:Cell Separation; D005456:Fluorescent Dyes; D015544:Inositol 1,4,5-Trisphosphate / Q000096:biosynthesis; D015759:Ionomycin / Q000494:pharmacology; D008407:Mast Cells / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000172:deficiency* / Q000378:metabolism / Q000502:physiology; D009092:Mucous Membrane / Q000378:metabolism; D010766:Phosphorylation; D051381:Rats; D017455:Receptors, IgE / Q000187:drug effects / Q000502:physiology; D013499:Surface Properties; D010738:Type C Phospholipases / Q000378:metabolism; D014443:Tyrosine / Q000378:metabolism; D014966:Xanthenes
chemical_list: D000814:Aniline Compounds; D002132:Calcium Radioisotopes; D005456:Fluorescent Dyes; D008562:Membrane Glycoproteins; D017455:Receptors, IgE; D014966:Xanthenes; C059715:Fluo-3; D014443:Tyrosine; D015759:Ionomycin; D015544:Inositol 1,4,5-Trisphosphate; D010738:Type C Phospholipases; D002118:Calcium