title: Neither dietary fructose, dextrose nor starch modifies in vitro glycerol release by adipocytes from streptozotocin-diabetic rats.
abstract: Because we found previously that fructose feeding could alter lipolytic responses to isoproterenol and insulin in normal rats, we studied the effects of the same diet in neonatal, streptozotocin-diabetic rats. Twenty-seven 5-wk-old diabetic Sprague-Dawley rats were fed a diet containing 57% carbohydrate as either fructose, dextrose or starch for 6 wk. At the end of the nutritional period, plasma glucose and insulin concentrations in fed rats were similar in the three diabetic groups. Plasma triacylglycerol concentrations were higher in the fructose-fed group than in the other two groups (P < 0.05). Neither the maximal adipocyte lipolytic response (fructose = 1147 +/- 165%, starch = 1823 +/- 329% and dextrose = 1287 +/- 239% of basal values) nor the sensitivity to isoproterenol (ED50) was changed by the dietary carbohydrate exchange. The maximal antilipolytic action of insulin (starch = 68 +/- 10%, dextrose = 41 +/- 13%, fructose = 95 +/- 29% of stimulated lipolysis values) was comparable in the three diet groups. Thus, 6 wk of fructose feeding in diabetic rats increased plasma triacylglycerol concentrations, but had no detectable effect on plasma glucose or insulin concentrations, isoproterenol-induced lipolysis or the antilipolytic action of insulin.
authors.affiliation: Department of Diabetes, Institut National de la Santé et de la Recherche Medicale U 341, Hôtel-Dieu Hospital, Paris, France.
authors.forename: J
authors.initials: J
authors.lastname: Luo
authors.forename: S W
authors.initials: SW
authors.lastname: Rizkalla
authors.forename: C
authors.initials: C
authors.lastname: Alamowitch
authors.forename: J
authors.initials: J
authors.lastname: Boillot
authors.forename: F
authors.initials: F
authors.lastname: Bruzzo
authors.forename: A
authors.initials: A
authors.lastname: Chevalier
authors.forename: G
authors.initials: G
authors.lastname: Slama
mesh_terms: D000273:Adipose Tissue / Q000187:drug effects* / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn; D001786:Blood Glucose / Q000378:metabolism; D001835:Body Weight / Q000187:drug effects; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D004040:Dietary Carbohydrates / Q000008:administration & dosage / Q000494:pharmacology*; D004435:Eating / Q000187:drug effects; D005632:Fructose / Q000008:administration & dosage / Q000494:pharmacology*; D005947:Glucose / Q000008:administration & dosage / Q000494:pharmacology*; D005990:Glycerol / Q000378:metabolism*; D007328:Insulin / Q000097:blood; D007545:Isoproterenol / Q000494:pharmacology; D008066:Lipolysis / Q000187:drug effects; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013213:Starch / Q000008:administration & dosage / Q000494:pharmacology*
chemical_list: D001786:Blood Glucose; D004040:Dietary Carbohydrates; D007328:Insulin; D005632:Fructose; D013213:Starch; D005947:Glucose; D007545:Isoproterenol; D005990:Glycerol