PI-specific phospholipase C "alpha" from sheep seminal vesicles is a proteolytic fragment of PI-PLC delta.
Phosphatidylinositide-specific phospholipase C enzymes (PLCs) catalyze the conversion of the phosphoinositides to biologically important signal transducing molecules. These enzymes may be grouped into "families" which share similar structures and modes of regulation. The existence of a structurally distinct family of PLC termed "alpha" has been recently called into question. In the current paper we show by immunoblotting experiments that PLC "alpha" from sheep seminal vesicles is recognized by monoclonal antibodies raised against the delta 1 isoform of bovine brain PLC, and appears to be derived from a higher molecular weight band at 85 kDa. We also show that antibodies raised against PLC alpha efficiently immunoprecipitate the 85-kDa PLC delta 1 isoform from bovine brain and Chinese hamster lung fibroblasts. These data provide strong evidence that the PLC alpha from sheep seminal vesicles is a proteolytic fragment of PLC delta 1. Thus, there is still no conclusive evidence for a separate "alpha" class of PLC.
Taylor
G D
GD
Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas 75235-8593.
Fee
J A
JA
Silbert
D F
DF
Hofmann
S L
SL
D000818:Animals; D000911:Antibodies, Monoclonal; D007527:Isoenzymes; D008297:Male; D009928:Organ Specificity; D010446:Peptide Fragments / Q000302:isolation & purification / Q000378:metabolism; D010716:Phosphatidylinositols / Q000378:metabolism*; D012669:Seminal Vesicles / Q000201:enzymology*; D012756:Sheep; D013045:Species Specificity; D010738:Type C Phospholipases / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism
D000911:Antibodies, Monoclonal; D007527:Isoenzymes; D010446:Peptide Fragments; D010716:Phosphatidylinositols; D010738:Type C Phospholipases