A protein from Daudi cell line conditioned medium induces ovarian dysgenesis in Drosophila melanogaster.
From a medium in which Daudi cells had been grown, we isolated by HPLC a protein that caused ovarian abnormalities in adult females of Drosophila melanogaster when injected into preblastoderm embryos. This protein, whose apparent M(r) is between 30,000 and 50,000, was found to be a moderately polar compound which is heat stable and whose activity is destroyed by acidification. The protein is characteristic of medium conditioned from Daudi cells.
Richard-Mercier
N
N
Laboratoire de Physiologie de la Reproduction, Université Pierre et Marie Curie, Paris, France.
Thomas-Orillard
M
M
Morinière
M
M
Porcheron
P
P
Soyez
D
D
Fellous
M
M
D000818:Animals; D002452:Cell Count; D002851:Chromatography, High Pressure Liquid; D017077:Culture Media, Conditioned / Q000737:chemistry; D004331:Drosophila melanogaster / Q000033:anatomy & histology*; D005260:Female; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008297:Male; D009363:Neoplasm Proteins / Q000302:isolation & purification / Q000502:physiology*; D010053:Ovary / Q000002:abnormalities; D013696:Temperature; D014407:Tumor Cells, Cultured; D001613:beta 2-Microglobulin / Q000502:physiology
D017077:Culture Media, Conditioned; D009363:Neoplasm Proteins; D001613:beta 2-Microglobulin