Interactions of Escherichia coli membrane lipoproteins with the murein sacculus.
Bifunctional cross-linking reagents were used to identify cell envelope proteins that interacted with the murein sacculus. This revealed that a number of [3H]leucine-labeled proteins and [3H]palmitate-labeled lipoproteins were reproducibly cross-linked to the sacculus in plasmolyzed cells. The results suggested that most of the cell envelope lipoproteins, and not only the murein lipoprotein, mediate interactions between the murein sacculus and the inner and/or outer membrane of the cell.
Leduc
M
M
Department of Microbiology, University of Connecticut Health Center, Farmington 06032.
Ishidate
K
K
Shakibai
N
N
Rothfield
L
L
D003432:Cross-Linking Reagents; D015180:Electrophoresis, Gel, Two-Dimensional; D004926:Escherichia coli / Q000378:metabolism* / Q000648:ultrastructure; D008074:Lipoproteins / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism*; D010457:Peptidoglycan / Q000378:metabolism*
D003432:Cross-Linking Reagents; D008074:Lipoproteins; D008565:Membrane Proteins; D010457:Peptidoglycan