[Neuroanatomical studies on the urine storage facilitatory areas in the cat brain. Part I. Input neuronal structures to the nucleus locus subcoaruleus and the nucleus radicularis pontis oralis].
Input neuronal structures to the nucleus locus subcoeruleus (LSC) and the nucleus reticularis pontis oralis (PoO) were investigated by the horseradish peroxidase (HRP) study in cats. Under halothane anesthesia, a double barreled electrode was inserted into the LSC where electrical stimulation increased bladder capacity and the external urethral sphincter muscle activity, and into the PoO where chemical stimulation with carbachol increased bladder capacity and decreased the external urethral sphincter muscle activity. After identification of these regions, the HRP was ionphoretically injected into the LSC or PoO. By injecting the HRP into the LSC, retrogradely HRP labeled cells were located broadly in the frontal, rectal, orbitalis, rostral cingulate, internal aspect of posterior sigmoidal and anterior sylvian gyli, nucleus corticomedialis of amygdala, lateral area of the hypothalamus, paraventricular hypothalamic nucleus, substantia nigra, periaqueductal gray, reticular formation of the mesencephalon, pons and medulla, cerebellar nuclei and intermediate gray of the spinal cord. By injecting the HRP into the PoO, retrogradely HRP labeled cells were located broadly in the frontal, rectal, orbitalis, internal aspect of the posterior sigmoidal and anterior sylvian gyli, lateral area of the hypothalamus, paraventricular hypothalamic nucleus, periaqueductal gray, reticular formation of the mesencephalon, pons and medulla, cerebellar nuclei and intermediate gray of the spinal cord. These areas where HRP labeled cells were located mostly corresponded to the areas where electrical stimulation evoked either bladder relaxation or contraction in the previous reports. The LSC and the PoO seem to perform important roles in the neuronal mechanism for urine storage, receiving the inputs which facilitate or inhibit micturition from the extended areas between the cerebral cortex and the sacral spinal cord.
Kohama
T
T
Department of Urology, Akita University School of Medicine.
D000818:Animals; D002217:Carbachol / Q000494:pharmacology; D002415:Cats; D004558:Electric Stimulation; D005260:Female; D008125:Locus Coeruleus / Q000033:anatomy & histology* / Q000502:physiology; D009474:Neurons / Q000502:physiology; D011149:Pons / Q000033:anatomy & histology* / Q000502:physiology; D014521:Urethra / Q000502:physiology*; D001743:Urinary Bladder / Q000502:physiology*; D014554:Urination
D002217:Carbachol