title: Fluorescamine-induced membrane permeability in mitochondria.
abstract: 1. Addition of fluorescamine (75 microM) to mitochondria induced an increase in membrane permeability. 2. The leakiness of the inner mitochondrial membrane is characterized by extensive release of accumulated Ca2+, collapse of the transmembrane potential, mitochondrial swelling and efflux of matrix proteins, among them, malate dehydrogenase. 3. These effects were diminished by supplementing the media with 1 mM phosphate, and partially prevented by Mg2+. 4. These results indicate that the primary amino groups of membrane components contribute, partially, to the maintenance of the permeability barrier in mitochondria.
authors.affiliation: Departamento de Bioquímica, Instituto Nacional de Cardiología, México, D.F.
authors.forename: E
authors.initials: E
authors.lastname: Chávez
authors.forename: C
authors.initials: C
authors.lastname: Zazueta
authors.forename: H
authors.initials: H
authors.lastname: Reyes-Vivas
authors.forename: J
authors.initials: J
authors.lastname: Pichardo
authors.forename: N
authors.initials: N
authors.lastname: Corona
authors.forename: A
authors.initials: A
authors.lastname: Uribe
authors.forename: R
authors.initials: R
authors.lastname: Chávez
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D005450:Fluorescamine / Q000494:pharmacology*; D007668:Kidney / Q000187:drug effects* / Q000648:ultrastructure; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism; D010539:Permeability / Q000187:drug effects; D010710:Phosphates / Q000378:metabolism; D051381:Rats
chemical_list: D010710:Phosphates; D005450:Fluorescamine; D002118:Calcium