title: Characterisation of secondary spermatocytes in the marbled newt (Triturus marmoratus).
abstract: Secondary spermatocytes in the marbled newt (Triturus marmoratus) were identified by means of cytophotometric quantification of nuclear DNA in the testicular lobules containing primary spermatocytes in advanced stages of the first meiotic division (from pachytene onwards) and newly formed spermatids. The nucleus of secondary spermatocytes has an intermediate size between that of primary spermatocytes and round spermatids and was characterised by the presence of abundant masses of dense chromatin and the absence of a nucleolus. At the prophase the chromatin masses were located at the periphery of the nucleus. The ultrastructural study revealed that the nuclear envelope displayed blebs and that the cytoplasm was very similar to that of primary spermatocytes including the presence of smooth endoplasmic reticulum whorls, multiple small dictyosomes, and a proacrosomal-like granule near the nucleus. This granule stained weakly with aqueous PTA and was observed in about 6% of secondary spermatocytes. Many of the mitochondria were grouped beneath the plasma membrane. Approximately 2% of these cells presented an axoneme which was not associated with other flagellar components.
authors.affiliation: Department of Cell Biology and Genetics, University of Alcalá de Henares, Madrid, Spain.
authors.forename: B
authors.initials: B
authors.lastname: Fraile
authors.forename: F J
authors.initials: FJ
authors.lastname: Sáez
authors.forename: J
authors.initials: J
authors.lastname: Codesal
authors.forename: R
authors.initials: R
authors.lastname: Paniagua
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000502:physiology / Q000648:ultrastructure; D003592:Cytophotometry / Q000379:methods; D003593:Cytoplasm / Q000648:ultrastructure; D004247:DNA / Q000032:analysis; D006651:Histocytochemistry; D008297:Male; D012455:Salamandridae / Q000033:anatomy & histology*; D013090:Spermatocytes / Q000502:physiology / Q000648:ultrastructure*; D013091:Spermatogenesis / Q000502:physiology
chemical_list: D004247:DNA