title: Effect of delta-lysin on the phase transitions of lipid assemblies.
abstract: X-ray small-angle diffraction, differential scanning calorimetry (DSC), and temperature scanning densitometry (TSD) were used to study the effect of delta-lysin on the phase transitions of lipid assemblies from 1,2-0-dixehadecyl-sn-glycero-3-phosphocholine (DHPC). The experiments were carried out in excess of water in a temperature range of 0-55 degrees C, and at low peptide concentrations between 10(-4) and 10(-2) moles peptide per mole phospholipid. The incorporation of delta-lysin into lipid assemblies alters the lipid structure without significant changes on the temperatures of phase transition from gel to liquid crystalline phase. The temperature of the main transition was nearly unaffected. A reduction in the transition volume of the lipids with increasing concentrations of delta-lysin was observed. The minor changes in these parameters were interpreted as long-range structural changes caused by the peptide incorporation. The results are discussed in terms of the concept of cooperative phase transition of entire clusters occurring within a membrane implying that relative stable domains of gel phase, and liquid crystalline phase co-exist.
authors.affiliation: Department of Biophysics, Bulgarian Academy of Sciences, Sofia.
authors.forename: V
authors.initials: V
authors.lastname: Neitchev
authors.forename: K
authors.initials: K
authors.lastname: Lohner
authors.forename: A
authors.initials: A
authors.lastname: Collotto
authors.forename: P
authors.initials: P
authors.lastname: Laggner
mesh_terms: D001426:Bacterial Proteins / Q000494:pharmacology*; D002152:Calorimetry, Differential Scanning; D003720:Densitometry; D006460:Hemolysin Proteins / Q000494:pharmacology*; D010742:Phospholipid Ethers / Q000737:chemistry*; D013696:Temperature; D014961:X-Ray Diffraction
chemical_list: C052298:1,2-dihexadecyl-sn-glycero-3-phosphocholine; D001426:Bacterial Proteins; D006460:Hemolysin Proteins; D010742:Phospholipid Ethers; C025994:delta hemolysin protein, Staphylococcus aureus