cDNA cloning of rat proteasome subunit RC1, a homologue of RING10 located in the human MHC class II region.
The nucleotide sequence of a cDNA that encodes a new subunit, named RCl, of rat proteasomes (multicatalytic proteinase complexes) has been determined. The polypeptide predicted from the open reading frame consisted of 208 amino acid residues with a calculated molecular mass of 23, 130, which is consistent with the size obtained by electrophoretic analysis of purified RCl. The partial amino acid sequences of several fragments of RCl, obtained by protein chemical analyses, were found to be in excellent accordance with those deduced from the cDNA sequence. Surprisingly, the overall structure of RCl was found to be almost identical to that of recently isolated RING10, whose gene is located in the class II region of the human MHC gene cluster. This finding suggests that RCl is a homologue of human RING10, supporting the proposal that proteasomes are involved in the antigen processing pathway.
Aki
M
M
Department of Urology, School of Medicine, University of Tokushima, Japan.
Tamura
T
T
Tokunaga
F
F
Iwanaga
S
S
Kawamura
Y
Y
Shimbara
N
N
Kagawa
S
S
Tanaka
K
K
Ichihara
A
A
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D003546:Cysteine Endopeptidases / Q000235:genetics*; D005802:Genes, MHC Class II / Q000235:genetics*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009097:Multienzyme Complexes / Q000235:genetics*; D016366:Open Reading Frames; D010446:Peptide Fragments / Q000737:chemistry / Q000302:isolation & purification; D046988:Proteasome Endopeptidase Complex; D051381:Rats; D017386:Sequence Homology, Amino Acid
D009097:Multienzyme Complexes; D010446:Peptide Fragments; D003546:Cysteine Endopeptidases; D046988:Proteasome Endopeptidase Complex