title: Detection and identification of mammalian DNA from the gut of museum specimens of ticks.
abstract: A method for detection and identification of mammalian DNA in the gut of alcohol-preserved ticks has been developed. Successful amplification and sequencing of host cytochrome b is reported from ticks preserved in 70% alcohol for more than 40 yr. The method described shows promise for determination of unknown tick hosts.
authors.affiliation: Department of Zoology, Brigham Young University, Provo, UT 84602.
authors.forename: J
authors.initials: J
authors.lastname: Tobolewski
authors.forename: M J
authors.initials: MJ
authors.lastname: Kaliszewski
authors.forename: R K
authors.initials: RK
authors.lastname: Colwell
authors.forename: J H
authors.initials: JH
authors.lastname: Oliver
mesh_terms: D000818:Animals; D001096:Arachnid Vectors; D001483:Base Sequence; D003573:Cytochrome b Group / Q000235:genetics; D004247:DNA / Q000235:genetics / Q000302:isolation & purification*; D008322:Mammals; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D013987:Ticks
chemical_list: D003573:Cytochrome b Group; D004247:DNA