Isolation of a novel nuclear glycoprotein from pig kidney.
A nuclear glycoprotein with an apparent Mr of 66,000 Da has been isolated from pig kidney chromatin after extraction with urea, guanidine-HCl and 2 M NaCl, and some of its structural features have been characterized. It belongs to the group of N-glycosylated proteins, which in the nucleus has so far received little attention. From its monosaccharide composition and recognition by lectins its oligosaccharides appear to be of high mannose and/or hybrid types. Some properties of its protein moiety suggest that it has a role in the packing of the DNA loops in the condensed chromatin.
Eufemi
M
M
Department of Biochemical Sciences, University La Sapienza, Rome, Italy.
Maras
B
B
Valiante
S
S
Altieri
F
F
Spoto
G
G
Turano
C
C
Ferraro
A
A
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000032:analysis; D002843:Chromatin / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D015180:Electrophoresis, Gel, Two-Dimensional; D006023:Glycoproteins / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D007668:Kidney / Q000737:chemistry*; D037102:Lectins / Q000378:metabolism; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013329:Structure-Activity Relationship; D013552:Swine
D000596:Amino Acids; D002241:Carbohydrates; D002843:Chromatin; D006023:Glycoproteins; D037102:Lectins; D009687:Nuclear Proteins