title: The effect of repeated instillations of antiseptics on catheter-associated urinary tract infections: a study in a physical model of the catheterized bladder.
abstract: The activity of three antiseptic bladder washout solutions was examined in a physical model of the catheterized bladder. Tests were performed against cultures of four common urinary tract pathogens that had established themselves in the model and colonized the surfaces with biofilm. Double instillations of chlorhexidine (0.02% w/v) at 6-h intervals failed to eliminate Pseudomonas aeruginosa, Proteus mirabilis, and Providencia stuartii from the bladder model. Escherichia coli, however, was susceptible to a second instillation provided that it was performed within 12 h. Supplementing chlorhexidine with EDTA and TRIS potentiated its activity against E. coli. Mandelic acid (1.0% w/v) was the most effective of the agents, double instillations eliminating all but Pr. mirabilis infections.
authors.affiliation: School of Pure and Applied Biology, University of Wales, College of Cardiff, UK.
authors.forename: J B
authors.initials: JB
authors.lastname: King
authors.forename: D J
authors.initials: DJ
authors.lastname: Stickler
mesh_terms: D000283:Administration, Intravesical; D000891:Anti-Infective Agents, Local / Q000008:administration & dosage*; D002710:Chlorhexidine / Q000008:administration & dosage; D004492:Edetic Acid / Q000008:administration & dosage; D004756:Enterobacteriaceae Infections / Q000188:drug therapy / Q000209:etiology; D004927:Escherichia coli Infections / Q000188:drug therapy / Q000209:etiology; D016905:Gram-Negative Bacterial Infections / Q000188:drug therapy* / Q000209:etiology; D006801:Humans; D066298:In Vitro Techniques; D008333:Mandelic Acids / Q000008:administration & dosage; D008954:Models, Biological; D011512:Proteus Infections / Q000188:drug therapy / Q000209:etiology; D011513:Proteus mirabilis; D011532:Providencia; D011552:Pseudomonas Infections / Q000188:drug therapy / Q000209:etiology; D014325:Tromethamine / Q000008:administration & dosage; D014546:Urinary Catheterization / Q000009:adverse effects*; D014552:Urinary Tract Infections / Q000188:drug therapy* / Q000209:etiology
chemical_list: D000891:Anti-Infective Agents, Local; D008333:Mandelic Acids; D014325:Tromethamine; D004492:Edetic Acid; C037938:mandelic acid; D002710:Chlorhexidine