title: Molecular cloning and sequencing of an alternatively spliced form of the human thyrotropin receptor transcript.
abstract: In the present study, we report the molecular cloning and sequencing of an alternatively spliced form of the human thyrotropin receptor (hTSHR) mRNA transcript, which has previously been detected on Northern blot analysis of human thyroid cells. The smaller hTSHR cDNA, designated hTSHR cDNA-I, is approximately 1 kb in size and encodes a protein of 253 amino acids. Comparison of the nucleotide sequence of hTSHR cDNA-I with available hTSHR genomic sequence data reveals that the cDNA-I contains exons 1-8 and unidentified DNA tract, presumably an intron. Thus, the hTSHR cDNA-I encodes for the N-terminal half of the extracellular domain of the hTSHR (approximately 60%). The truncated TSHR-I may be secreted and function as a TSH binding protein.
authors.affiliation: First Department of Internal Medicine, Nagasaki University School of Medicine, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Takeshita
authors.forename: Y
authors.initials: Y
authors.lastname: Nagayama
authors.forename: K
authors.initials: K
authors.lastname: Fujiyama
authors.forename: N
authors.initials: N
authors.lastname: Yokoyama
authors.forename: H
authors.initials: H
authors.lastname: Namba
authors.forename: S
authors.initials: S
authors.lastname: Yamashita
authors.forename: M
authors.initials: M
authors.lastname: Izumi
authors.forename: S
authors.initials: S
authors.lastname: Nagataki
mesh_terms: D017398:Alternative Splicing; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D006801:Humans; D008969:Molecular Sequence Data; D012322:RNA Precursors / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis*; D011989:Receptors, Thyrotropin / Q000235:genetics*
chemical_list: D012322:RNA Precursors; D012333:RNA, Messenger; D011989:Receptors, Thyrotropin