High growth reassortant influenza vaccine viruses: new approaches to their control.
When a new strain of an influenza virus is required to be incorporated into influenza vaccine, attempts are made to recombine such strains with laboratory adapted viruses, which will grow to high titre in order to improve the yield of the vaccine strain. It is important that such high growth reassortant vaccine strains are not contaminated with genes coding for the antigenic determinants of the high growth laboratory strain. We describe the characterization of two recent high growth reassortants and the application of the polymerase chain reaction to ensure their genetic identity and purity.
Robertson
J S
JS
National Institute for Biological Standards and Control, Potters Bar, Herts, U.K.
Nicolson
C
C
Newman
R
R
Major
D
D
Dunleavy
U
U
Wood
J M
JM
D000956:Antigens, Viral / Q000235:genetics; D001483:Base Sequence; D004279:DNA, Viral / Q000235:genetics; D006801:Humans; D009980:Influenza A virus / Q000235:genetics / Q000254:growth & development / Q000276:immunology*; D009981:Influenza B virus / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D007252:Influenza Vaccines / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D010641:Phenotype; D016133:Polymerase Chain Reaction; D011995:Recombination, Genetic; D014776:Virus Cultivation
D000956:Antigens, Viral; D004279:DNA, Viral; D007252:Influenza Vaccines; D015345:Oligonucleotide Probes