title: The acyl-CoA synthetase inhibitor triacsin C enhanced eicosanoid release in leukocytes.
abstract: Triacsin C was previously reported to inhibit long-chain acyl-CoA synthetase and to reduce the production of acyl-CoA in rat neutrophils dose- and time-dependently. We found that preincubation with triacsin C inhibited the incorporation of labeled arachidonic acid into rat neutrophils. Furthermore, when triacsin C-treated neutrophils were stimulated with the Ca-ionophore A23187, they released an increased amount of eicosanoids into the culture medium. These results indicate that triacsin C suppressed acylation of arachidonic acid, which resulted in an increase in its metabolites.
authors.affiliation: Department of Pharmacology, School of Pharmaceutical Sciences, Kitasato University, Tokyo, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Oh-ishi
authors.forename: K
authors.initials: K
authors.lastname: Yamaki
authors.forename: M
authors.initials: M
authors.lastname: Abe
authors.forename: H
authors.initials: H
authors.lastname: Tomoda
authors.forename: S
authors.initials: S
authors.lastname: Omura
mesh_terms: D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism; D002250:Carbon Radioisotopes; D003066:Coenzyme A Ligases / Q000037:antagonists & inhibitors*; D015777:Eicosanoids / Q000378:metabolism*; D066298:In Vitro Techniques; D007962:Leukocytes / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D014226:Triazenes / Q000494:pharmacology*; D014665:Vasodilator Agents / Q000494:pharmacology
chemical_list: D002250:Carbon Radioisotopes; D015777:Eicosanoids; D014226:Triazenes; D014665:Vasodilator Agents; D016718:Arachidonic Acid; C034613:triacsin C; D003066:Coenzyme A Ligases