Talin anchors and nucleates actin filaments at lipid membranes. A direct demonstration.
Platelet talin nucleates actin assembly as we show here directly by using rhodamine-phalloidin labelling of actin filaments. Nucleation by talin still occurs after reconstitution into liposomal bilayers. This is also demonstrated directly after protein-lipid double labelling and light microscopic imaging. Talin, thus, is the first actin binding protein for which anchoring and nucleation of actin filament growth at lipid interfaces have been visualized.
Kaufmann
S
S
Biophysics Department, Technical University of Munich, Garching, Germany.
Käs
J
J
Goldmann
W H
WH
Sackmann
E
E
Isenberg
G
G
D008841:Actin Cytoskeleton / Q000378:metabolism* / Q000648:ultrastructure; D000199:Actins / Q000302:isolation & purification / Q000378:metabolism*; D006651:Histocytochemistry; D006801:Humans; D008081:Liposomes / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D010713:Phosphatidylcholines; D010718:Phosphatidylserines; D016608:Talin / Q000378:metabolism*
D000199:Actins; D008081:Liposomes; D010713:Phosphatidylcholines; D010718:Phosphatidylserines; D016608:Talin