N epsilon(gamma-glutamyl)lysine crosslinks in the blood clot of the horseshoe crab, Limulus polyphemus.
Clots were allowed to form in samples of whole blood taken from the American horseshoe crab, Limulus polyphemus, in the absence and presence of dansylcadaverine (16), and were analyzed for their contents of N epsilon(gamma-glutamyl)lysine and gamma-glutamyl-dansylcadaverine. Clots obtained without dansylcadaverine yielded significant amounts of N epsilon(gamma-glutamyl)lysine product. Clots formed in the presence of dansylcadaverine yielded only gamma-glutamyl-dansylcadaverine. Formation of these products reflects on the activity of transglutaminase released from the blood cells during coagulation.
Wilson
J
J
Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston, IL 60208.
Rickles
F R
FR
Armstrong
P B
PB
Lorand
L
L
D000818:Animals; D001777:Blood Coagulation; D002103:Cadaverine / Q000031:analogs & derivatives / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D004151:Dipeptides / Q000032:analysis / Q000378:metabolism*; D006737:Horseshoe Crabs / Q000502:physiology*; D007202:Indicators and Reagents
D004151:Dipeptides; D007202:Indicators and Reagents; C001299:epsilon-(gamma-glutamyl)-lysine; C008542:monodansylcadaverine; D002103:Cadaverine