title: Inhibition by spermine of the inner membrane permeability transition of isolated rat heart mitochondria.
abstract: The effect of spermine on the permeability transition of the inner mitochondrial membrane of isolated rat heart mitochondria was evaluated. The permeability transition was triggered using a series of agents (t-butyl hydroperoxide, phenylarsine oxide, carboxyatractylate, and elevated Ca2+ and inorganic phosphate concentrations), and was monitored via Ca(2+)-release, mitochondrial swelling and pyridine nucleotide oxidation. By all three criteria, spermine inhibited the transition. A C50 of 0.38 +/- 0.06 (SD) mM was measured for inhibition.
authors.affiliation: Department of Pharmacology and Experimental Therapeutics, University of Maryland School of Medicine, Baltimore 21201.
authors.forename: R G
authors.initials: RG
authors.lastname: Lapidus
authors.forename: P M
authors.initials: PM
authors.lastname: Sokolove
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D066298:In Vitro Techniques; D008297:Male; D008929:Mitochondria, Heart / Q000187:drug effects*; D008933:Mitochondrial Swelling / Q000187:drug effects*; D010539:Permeability / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D013096:Spermine / Q000494:pharmacology*
chemical_list: D013096:Spermine; D002118:Calcium