[The development of diploid parthenogenetic mouse embryos of the inbred C57BL and CBA strains].
We studied preimplantation development in vitro and postimplantation development in vivo of diploid parthenogenetic mouse embryos of C57BL/6 and CBA strains, as well as of (CBA x C57BL/6)F1 hybrids. Development to blastocyst stage of diploid eggs obtained from C57BL/6, CBA, and hybrid mice was observed in 90, 15, and 73% cases, respectively. After implantation, C57BL/6 embryos did not develop to somite stages, while CBA and hybrid embryos reached various stages of somite formation in 45 and 30% cases, respectively. Cultivation of embryos beginning from one-cell stage in the medium containing 2% newborn calf serum increased the yield of blastocysts from 15 to 59% in CBA embryos and from 73 to 90% in hybrids; However, such effect was not observed with C57BL/6 embryos. The latest stages of development observed in CBA and hybrid diploid parthenogenetic embryos were 33-35 somites and 25-30 somites, respectively. Imprinting patterns in chromosomes of CBA and C57BL/6 gametes are discussed.
Penkov
L I
LI
Platonov
E S
ES
D000818:Animals; D003433:Crosses, Genetic; D004171:Diploidy; D004624:Embryo Transfer; D047108:Embryonic Development; D005314:Embryonic and Fetal Development; D005260:Female; D051379:Mice; D008810:Mice, Inbred C57BL / Q000196:embryology*; D008808:Mice, Inbred CBA / Q000196:embryology*; D010312:Parthenogenesis; D011247:Pregnancy; D013045:Species Specificity
Izuchenie razvitiia diploidnykh partenogeneticheskikh zarodysheĭ mysheĭ inbrednykh liniĭ C57BL i CBA.