title: Induction of melanin biosynthesis in Vibrio cholerae.
abstract: Vibrio cholerae synthesized the pigment melanin in response to specific physiological conditions that were stressful to the bacterium. Pigmentation was induced when V. cholerae was subjected to hyperosmotic stress in conjunction with elevated growth temperatures (above 30 degrees C). The salt concentration tolerated by V. cholerae was lowered by additional abiotic factors such as acidic starting pH of the growth medium and limitation of organic nutrients. Although the amount of toxin detected in the culture supernatant decreased significantly in response to stressful culture conditions, no correlation between the physiological conditions that induced melanogenesis and expression of OmpU or cholera toxin was detected. Since conditions that induce melanin production in V. cholerae occur in both the aquatic environment and the human host, it is possible that melanogenesis has a specific function with respect to the survival of the bacterium in these habitats.
authors.affiliation: Center of Marine Biotechnology, University of Maryland, Baltimore 21202.
authors.forename: V E
authors.initials: VE
authors.lastname: Coyne
authors.forename: L
authors.initials: L
authors.lastname: al-Harthi
mesh_terms: D001622:Betaine / Q000494:pharmacology; D003470:Culture Media; D015964:Gene Expression Regulation, Bacterial; D006863:Hydrogen-Ion Concentration; D008543:Melanins / Q000096:biosynthesis* / Q000502:physiology; D009994:Osmolar Concentration; D011392:Proline / Q000494:pharmacology; D012965:Sodium Chloride / Q000494:pharmacology; D013696:Temperature; D014734:Vibrio cholerae / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*
chemical_list: D003470:Culture Media; D008543:Melanins; D001622:Betaine; D012965:Sodium Chloride; D011392:Proline