[The use of cryopreserved swine blood for in vitro feeding of the soft tick Ornithodoros moubata].
Porcine stored blood has been used in the in vitro feeding of the soft tick Ornithodoros moubata through a Parafilm membrane. The efficiency of the feeding decreased after storage of 5 weeks by -20 degrees C. Through addition of 10(-3) M ATP/l the feeding results with frozen blood for duration of storage up to 200 days well agreed with the results of feeding tests with fresh porcine blood: The nymphs (N)1-3 fed the 4-5fold, the N4-6 the 2.7fold and the adults (A) the 1.9fold of their body weight. The feeding rate was 98.6% (N1-3); 92.7% (N4-5) and 81.9% (A). The mortality rate was under 10%. Females laid 130 eggs after blood meal.
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Institut für Parasitologie des Fachbereiches Veterinärmedizine, Humboldt-Universität zu Berlin, Deutschland.
Matthes
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HF
Hiepe
T
T
D000818:Animals; D001793:Blood Preservation; D015925:Cryopreservation; D003470:Culture Media; D005260:Female; D009758:Nymph / Q000254:growth & development; D010058:Oviposition; D013552:Swine / Q000097:blood*; D013987:Ticks / Q000254:growth & development* / Q000502:physiology
D003470:Culture Media
Einsatz gefrierkonservierten Schweineblutes zur In-vitro-Ernährung der Lederzecke Ornithodoros moubata.