Cross-reacting antigens in the butter clam (Saxidoma giganteus) and their relationship to total paralytic shellfish poison toxicity.
A specific protein with an apparent mol. wt of 23,000 was identified in foot homogenate derived from paralytic shellfish poisoning (PSP) contaminated butter clams and was found to cross-react with crab-saxitoxin-induced protein (SIP) antiserum. Antiserum, once cross-absorbed against non-toxic shellfish material, was incubated with tissue homogenate derived from 52 butter clams with varying total PSP toxicities in a prototype ELISA. A significant (r = 0.83; P less than 0.001) correlation existed between soluble clam antigen content in foot homogenate and total PSP toxicity; the latter measured by the mouse lethality bioassay. From the ELISA results, a soluble antigen threshold of 0.1% total protein was successfully used to distinguish between PSP toxic and non-toxic butter clams. It is proposed that this type of screening assay could be used in conjunction with the standard mouse bioassay to increase PSP monitoring and potentially reduce unnecessary animal testing.
Kitts
D D
DD
Department of Food Science, University of British Columbia, Vancouver, Canada.
Smith
D S
DS
D000818:Animals; D000918:Antibody Specificity; D000941:Antigens / Q000276:immunology*; D049872:Bivalvia / Q000276:immunology*; D015153:Blotting, Western; D002846:Chromatography, Affinity; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D007074:Immunoglobulin G / Q000276:immunology; D008387:Marine Toxins / Q000633:toxicity*; D010243:Paralysis / Q000139:chemically induced*; D011506:Proteins / Q000276:immunology / Q000633:toxicity; D012758:Shellfish / Q000032:analysis*
D000941:Antigens; D007074:Immunoglobulin G; D008387:Marine Toxins; D011506:Proteins