Proteinases of Trichomonas vaginalis: antibody response in patients with urogenital trichomoniasis.
Immunoprecipitation combined with electrophoresis in gelatin-polyacrylamide gels was successfully used for detection of antibodies against numerous proteinases of Trichomonas vaginalis in infected patients. The method proved to be highly specific as anti-proteinase antibodies were absent in women with negative cultivation of T. vaginalis and no history of trichomoniasis. Sera of 71% and vaginal washes of 86% patients with trichomoniasis were positive for these antibodies. In vaginal washes, but not in sera, antibodies were partly complexed with proteinases, possibly of trichomonad origin. It was also shown that serum antibodies as well as local anti-proteinase antibodies persisted for weeks after patients had been cured.
Bózner
P
P
Institute of Microbiology and Immunology, Medical Faculty, Comenius University, Bratislava, Czechoslovakia.
Gombosová
A
A
Valent
M
M
Demes
P
P
Alderete
J F
JF
D000328:Adult; D000818:Animals; D000913:Antibodies, Protozoan / Q000032:analysis* / Q000097:blood; D004591:Electrophoresis, Polyacrylamide Gel; D010450:Endopeptidases / Q000032:analysis / Q000276:immunology*; D005260:Female; D006801:Humans; D008875:Middle Aged; D011233:Precipitin Tests; D014247:Trichomonas Vaginitis / Q000276:immunology*; D014246:Trichomonas vaginalis / Q000201:enzymology / Q000276:immunology*; D014621:Vagina / Q000201:enzymology / Q000276:immunology / Q000469:parasitology
D000913:Antibodies, Protozoan; D010450:Endopeptidases