title: Cholesterol ozonation products as biomarkers for ozone exposure in rats.
abstract: Cholesterol, 1, which is present in both the lung lining fluid and cell membranes of lung tissue, reacts with ozone in aqueous systems to give 3 beta-hydroxy-5-oxo-5,6-secocholestan-6-al (2) as the major product. Reaction of 2 with 2,4-dinitrophenyl hydrazine (DNPH) in aqueous solutions, liposomes or lung extracts affords the anti and syn DNPH derivatives of 2 (3b and 3c) and of the rearrangement product 3,5-dihydroxy-B-norcholestane-6-carboxaldehyde (3a). These derivatives also are detected in lung tissue extracts from rats exposed to 1.3 ppm ozone for 12 hr.
authors.affiliation: Biodynamics Institute, Louisiana State University, Baton Rouge 70803-1800.
authors.forename: W A
authors.initials: WA
authors.lastname: Pryor
authors.forename: K
authors.initials: K
authors.lastname: Wang
authors.forename: E
authors.initials: E
authors.lastname: Bermúdez
mesh_terms: D015060:1,2-Dipalmitoylphosphatidylcholine; D000818:Animals; D002784:Cholesterol / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D066298:In Vitro Techniques; D008081:Liposomes; D008168:Lung / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D010126:Ozone / Q000633:toxicity*; D010713:Phosphatidylcholines; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D008081:Liposomes; D010713:Phosphatidylcholines; D015060:1,2-Dipalmitoylphosphatidylcholine; D010126:Ozone; D002784:Cholesterol