Purification of the H(+)-ATPase from chloroplasts.
Triton X-100* was replaced in the purification of the H(+)-ATPase from chloroplasts by either octyl glucoside, octyl thio glucoside or the zwitterionic detergent CHAPS. The results indicate that the enzyme can be purified without Triton X-100 and retain full activity.
Kirch
R D
RD
Biologisches Institut, Universität Stuttgart, Germany.
Gräber
P
P
D002736:Chloroplasts / Q000201:enzymology*; D002793:Cholic Acids; D003902:Detergents; D005960:Glucosides; D017830:Octoxynol; D010945:Plants, Edible / Q000201:enzymology*; D011092:Polyethylene Glycols; D006180:Proton-Translocating ATPases / Q000302:isolation & purification* / Q000378:metabolism; D012997:Solvents; D013863:Thioglucosides
D002793:Cholic Acids; D003902:Detergents; D005960:Glucosides; D012997:Solvents; D013863:Thioglucosides; C018619:octyl-beta-D-glucoside; D011092:Polyethylene Glycols; C042989:n-octyl-beta-D-thioglucopyranoside; D017830:Octoxynol; D006180:Proton-Translocating ATPases; C028213:3-((3-cholamidopropyl)dimethylammonium)-1-propanesulfonate