The effect of a histidine-excess diet on cholesterol synthesis and degradation in rats.
Feeding a diet high in excess histidine (5% L-histidine) resulted in hypercholesterolemia and enlargement of the liver in rats. To clarify the mechanism of the hypercholesterolemia, cholesterol synthesis and degradation were followed. We found that hepatic 3-hydroxy-3-methylglutaryl-coenzyme A reductase activity in histidine-excess diet rats was significantly higher than in rats fed a basal diet. Incorporation of [3H] water into cholesterol of liver slices from rats fed the histidine-excess diet was higher than incorporation into liver slices from rats fed the basal diet (expressed per liver per 100 g body weight). In vivo incorporation of [3H] water into hepatic cholesterol was also higher, but the incorporation into cholesterol of the small intestine was lower in histidine-fed rats than in rats fed the basal diet (expressed per liver per 100 g body weight). Hepatic cholesterol 7 alpha-hydroxylase activity was similar in both groups. The data suggest that the hypercholesterolemia caused by histidine-excess diet appears to be due to the stimulation of cholesterol synthesis in the liver.
Hitomi-Ohmura
E
E
Department of Agricultural Chemistry, School of Agriculture, Nagoya University, Japan.
Amano
N
N
Aoyama
Y
Y
Yoshida
A
A
D000818:Animals; D002784:Cholesterol / Q000096:biosynthesis* / Q000097:blood / Q000378:metabolism; D002790:Cholesterol 7-alpha-Hydroxylase / Q000378:metabolism; D004032:Diet / Q000009:adverse effects*; D006639:Histidine / Q000008:administration & dosage*; D006903:Hydroxymethylglutaryl CoA Reductases / Q000378:metabolism; D006937:Hypercholesterolemia / Q000209:etiology; D066298:In Vitro Techniques; D008099:Liver / Q000378:metabolism; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013261:Sterols / Q000378:metabolism; D014867:Water / Q000378:metabolism
D013261:Sterols; D014867:Water; D006639:Histidine; D002784:Cholesterol; D006903:Hydroxymethylglutaryl CoA Reductases; D002790:Cholesterol 7-alpha-Hydroxylase