Age related rejoining of broken chromosomes in human leukocytes following X-irradiation.
One theory of aging is that the ability to repair DNA or chromosome damage decreases with age. This study made use of a method which allows the measurement of chromatid repair in interphase nuclei, the technique of premature chromosome condensation. The number of excess chromosome fragments per cell remaining after 24 h of incubation at 37 degrees C was determined and used as a measure of the ability of leukocytes to repair. The leukocytes from male donors of several ages were used and the cells from older donors showed greater residual damage and, therefore, less repair.
Maillie
H D
HD
University of Rochester, Department of Biophysics, NY 14642.
Baker
J V
JV
Simon
W
W
Watts
R J
RJ
Quinn
B R
BR
D000293:Adolescent; D000328:Adult; D000368:Aged; D000375:Aging; D000818:Animals; D016466:CHO Cells; D002678:Chimera; D002875:Chromosomes / Q000528:radiation effects*; D006224:Cricetinae; D004260:DNA Repair; D006801:Humans; D007399:Interphase; D007962:Leukocytes / Q000000981:diagnostic imaging; D008297:Male; D008875:Middle Aged; D011859:Radiography