Nucleotide sequence of the bovine interleukin-6 gene promoter.
We report the cloning and sequencing of a 1252 base pairs (bp) DNA fragment containing the bovine interleukin-6 (IL-6) gene promoter. This fragment was isolated from a bovine genomic library constructed in the lambda GEM11 vector. Comparison with human, murine and rat IL-6 gene promoters reveals a high degree of conservation of the 200 bp immediately upstream of the RNA CAP site. This region contains nucleotide stretches matching with consensus sequences recognized by transcription factors, including NF-KB, CREB and NF-IL6. A potential AP-1 binding site is found 284 nucleotides upstream of the RNA CAP site. The bovine IL-6 gene promoter cloned upstream of the bacterial chloramphenicol acetyl transferase (CAT) gene was shown to be active in bovine and ovine cells.
Droogmans
L
L
Laboratoire de Chimie Biologique, Université Libre de Bruxelles, Rhode-St-Genèse, Belgium.
Cludts
I
I
Cleuter
Y
Y
Kettmann
R
R
Burny
A
A
D000818:Animals; D001483:Base Sequence; D002417:Cattle; D003001:Cloning, Molecular; D004247:DNA; D006801:Humans; D015850:Interleukin-6 / Q000235:genetics*; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012689:Sequence Homology, Nucleic Acid
D015850:Interleukin-6; D004247:DNA