Stimulation of myenteric plexus neurite outgrowth by insulin and insulin-like growth factors I and II.
A defined culture medium containing insulin, insulin-like growth factor I (IGF-I) or insulin-like growth factor II (IGF-II) supported morphological development of myenteric plexus neurons derived from neonatal guinea pigs. Insulin increased neurite outgrowth 3-fold at concentrations as low as 0.2 nM. Similar significant and dose-dependent increases in neurite outgrowth were noted with IGF-I and IGF-II. Stimulation of neurite outgrowth was abolished by exposure to cytosine arabinofuranoside, an agent toxic to non-neuronal cells, implying that trophic effects of insulin or insulin-like growth factors require the presence of non-neuronal elements in culture.
Mulholland
M W
MW
Michigan Gastrointestinal Peptide Center, University of Michigan, Ann Arbor 48109-0331.
Romanchuk
G
G
Simeone
D M
DM
Flowe
K
K
D000818:Animals; D002452:Cell Count; D002478:Cells, Cultured; D003470:Culture Media; D003561:Cytarabine / Q000494:pharmacology; D006168:Guinea Pigs; D007328:Insulin / Q000494:pharmacology*; D007329:Insulin Antagonists; D007334:Insulin-Like Growth Factor I / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D007335:Insulin-Like Growth Factor II / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D008297:Male; D009197:Myenteric Plexus / Q000187:drug effects*; D016501:Neurites / Q000187:drug effects* / Q000502:physiology*
D003470:Culture Media; D007328:Insulin; D007329:Insulin Antagonists; D003561:Cytarabine; D007334:Insulin-Like Growth Factor I; D007335:Insulin-Like Growth Factor II