The two mRNAs encoding rat intestinal alkaline phosphatase represent two distinct nucleotide sequences.
Rat duodenal mucosa contains two mRNAs (2.7 and 3.0 kb) encoding intestinal alkaline phosphatase (IAP), but the mechanism for their production has not been clear. By means of the polymerase chain reaction (PCR), we isolated a fragment that identifies a second rat IAP (rIAP-II), differing from the rIAP-I sequence in the coding and 3' untranslated regions and encoding a COOH-terminal sequence predicted to be hydrophilic. By means of probes unique to each sequence, rIAP-I identified the 2.7-kb mRNA, and rIAP-II the 3.0-kb mRNA. The different structures and differential regulation of the mRNAs after fat feeding demonstrate the presence of two rat IAP transcripts.
Engle
M J
MJ
Department of Medicine, Washington University School of Medicine, St. Louis, MO 63110.
Alpers
D H
DH
D000469:Alkaline Phosphatase / Q000737:chemistry / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000737:chemistry; D004386:Duodenum / Q000201:enzymology*; D007413:Intestinal Mucosa / Q000201:enzymology*; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley
D012333:RNA, Messenger; D004247:DNA; D000469:Alkaline Phosphatase