The purification and sequence analysis of an avian neuromedin U.
In this study we have purified an avian homologue of neuromedin U from the chicken. Each step of the purification process was followed by a specific radioimmunoassay developed using porcine neuromedin U. Microsequence analysis characterised the peptide to be 25 amino acid residues long with the following sequence: Y-K-V-D-E-D-L-Q-G-A-G-G-I-Q-S-R-G-Y-F-F-F-R-P-R-N. Chicken neuromedin U has marked sequence similarity with the porcine peptide at its bioactive C-terminal region. Our findings demonstrate that the amino acid sequence of neuromedin U is markedly conserved in species which have diverged millions of years ago in evolutionary terms.
Domin
J
J
Department of Medicine, Royal Postgraduate Medical School, Hammersmith Hospital, London, UK.
Benito-Orfila
M A
MA
Nandha
K A
KA
Aitken
A
A
Bloom
S R
SR
D000595:Amino Acid Sequence; D000818:Animals; D002645:Chickens; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D007421:Intestine, Small / Q000737:chemistry*; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000737:chemistry* / Q000302:isolation & purification; D011863:Radioimmunoassay; D017421:Sequence Analysis
D009479:Neuropeptides; C056900:neuromedin U