title: Initial results of endothelial cell seeding following argon laser carotid endarterectomy.
abstract: This study evaluates the initial results of endothelial cell (EC) seeding following argon laser carotid endarterectomy. Venous endothelial cells were harvested from 12 dogs and cultured. A laser endarterectomy was performed on both carotids of each dog. One side was seeded with endothelial cells. Six dogs had both carotids harvested 1 hour after restoring blood flow. The others were harvested in 24 hours. The percentage of lumen covered with EC was evaluated by scanning electron microscopy. At 1 hour, the seeded arteries demonstrated 35 +/- 3 percent EC coverage, whereas the unseeded arteries had no EC coverage (P = 0.0002). At 24 hours, the seeded arteries had 58 +/- 15 percent EC coverage, whereas the unseeded arteries had no coverage (P = 0.01). Significant gross thrombus developed only in unseeded arteries (P = 0.047), two of which were occluded at 24 hours. EC seeding is beneficial following argon laser carotid endarterectomy resulting in improved patency and less surface thrombogenicity.
authors.affiliation: Department of Surgery, Indiana University Medical Center, Indianapolis 46202.
authors.forename: A P
authors.initials: AP
authors.lastname: Sawchuk
authors.forename: M B
authors.initials: MB
authors.lastname: Herring
authors.forename: M C
authors.initials: MC
authors.lastname: Dalsing
mesh_terms: D000818:Animals; D002339:Carotid Arteries / Q000601:surgery / Q000648:ultrastructure; D002340:Carotid Artery Diseases / Q000517:prevention & control*; D002478:Cells, Cultured; D004285:Dogs; D016894:Endarterectomy, Carotid; D004730:Endothelium, Vascular / Q000166:cytology / Q000648:ultrastructure; D053685:Laser Therapy; D008855:Microscopy, Electron, Scanning; D013499:Surface Properties; D013927:Thrombosis / Q000517:prevention & control*; D014654:Vascular Patency