title: Determination of protein concentrations and their molecular weight in tears from cats with normal corneas and cats with corneal sequestrum.
abstract: Protein concentration was determined, using the Bradford technique, in tears from cats with normal corneas and from cats with corneal sequestrum. Tears from the former group contained 5.81 +/- 2.29 mg of protein/ml; those from corneal sequestrum-affected cats contained 6.21 +/- 2.21 mg/ml. Difference between the 2 values was not significant. Molecular weight determination was made, using 4 to 20% sodium dodecyl sulfate-polyacrylamide gels. Molecular mass of proteins ranged from 263 to 14 kDa. There was no detectable difference in the band patterns for the 2 groups.
authors.affiliation: Department of Immunology and Microbiology, Wayne State University School of Medicine, Detroit, MI 48201.
authors.forename: H J
authors.initials: HJ
authors.lastname: Davidson
authors.forename: J A
authors.initials: JA
authors.lastname: Gerlach
authors.forename: R W
authors.initials: RW
authors.lastname: Bull
mesh_terms: D000818:Animals; D002371:Cat Diseases / Q000378:metabolism*; D002415:Cats; D003316:Corneal Diseases / Q000378:metabolism / Q000662:veterinary*; D004589:Electrophoresis, Disc / Q000662:veterinary; D005136:Eye Proteins / Q000378:metabolism*; D008970:Molecular Weight; D010865:Pilot Projects; D013666:Tears / Q000737:chemistry*
chemical_list: D005136:Eye Proteins