title: Crystallization and preliminary crystallographic investigation of methanol dehydrogenase from Methylobacterium extorquens AM1.
abstract: Single crystals of methanol dehydrogenase (MDH) from Methylobacterium extorquens AM1 have been grown by the vapour diffusion method. These crystals diffract to beyond 2 A resolution and are suitable for X-ray crystallography. They belong to the orthorhombic space group P2(1)2(1)2(1) and have the following unit cell parameters: a = 66.79 A, b = 108.9 A, c = 188.9 A. One asymmetric unit contains an alpha 2 beta 2 tetramer of MDH and the location of the non-crystallographic 2-fold symmetry axis of this tetramer is defined by the paired positions of the binding sites of heavy atoms in four MDH-derivatives.
authors.affiliation: Laboratory of Molecular Biophysics, University of Oxford, U.K.
authors.forename: M
authors.initials: M
authors.lastname: Ghosh
authors.forename: K
authors.initials: K
authors.lastname: Harlos
authors.forename: C C
authors.initials: CC
authors.lastname: Blake
authors.forename: I
authors.initials: I
authors.lastname: Richardson
authors.forename: C
authors.initials: C
authors.lastname: Anthony
mesh_terms: D000429:Alcohol Oxidoreductases / Q000737:chemistry*; D003460:Crystallization; D006088:Gram-Negative Aerobic Bacteria / Q000201:enzymology*; D014961:X-Ray Diffraction
chemical_list: D000429:Alcohol Oxidoreductases; C019641:alcohol dehydrogenase (acceptor)