An analytical study of antibacterial residues in meat: the simultaneous determination of 23 antibiotics and 13 drugs using gas chromatography.
Simultaneous determination of 7 penicillins, 3 tetracyclines, 5 aminoglycoside antibiotics, 5 macrolide antibiotics, 2 polyether antibiotics, chloramphenicol and 13 drugs was evaluated. The 36 agents in meat were extracted with 10% trichloroacetic acid. The extracted solutions were adsorbed on an Amberlite XAD-2 resin column and an activated carbon resin column and then eluted with methanol and 0.01N HCl-methanol, respectively. The eluted solutions were concentrated to dryness and trimethylsilylated with pyridine, N, 0-bis (trimethylsilyl) acetamide, N-trimethylsilylimidazole and trimethylchlorosilane. They were then measured by gas chromatography with a flame-ionization detector. The recovery for almost all of the 36 compounds from the original meat specimens was more than 82%. The limits of detection for most of the 36 agents using this method were equivalent to the original individual methods of analysis.
Mineo
H
H
Atsugi Health Center, Prefecture of Kanagawa, Japan.
Kaneko
S
S
Koizumi
I
I
Asida
K
K
Akahori
F
F
D000617:Aminoglycosides; D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis*; D002417:Cattle; D002849:Chromatography, Gas / Q000379:methods; D005506:Food Contamination / Q000032:analysis*; D008460:Meat / Q000032:analysis*; D010406:Penicillins / Q000032:analysis*; D013424:Sulfanilamides / Q000032:analysis*; D013552:Swine; D013754:Tetracyclines / Q000032:analysis*
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D010406:Penicillins; D013424:Sulfanilamides; D013754:Tetracyclines