Induction of a Streptomyces cacaoi beta-lactamase gene cloned in S. lividans.
The previously cloned class A beta-lactamase gene (bla) of Streptomyces cacaoi was shown to be inducible by beta-lactam compounds in the host organism S. lividans. A regulatory region of 2.75 kb was identified and the nucleotide sequence determined. It contained four open reading frames (ORFs) of which only two were complete and required for induction. ORF1-ORF2 exerted a positive regulatory effect on the expression of bla. Inactivation of ORF1 or of ORF2 resulted not only in the loss of induction, but also in a 30- to 60-fold decrease in the basal (non-induced) level of beta-lactamase production. ORF1 codes for a DNA-binding protein related to the AmpR repressor/activator, which controls the expression of ampC (class C beta-lactamase) genes in several Enterobacteria.
Lenzini
V M
VM
Centre d'Ingénierie des Protéines, Université de Liège, Sart Tilman, Belgium.
Magdalena
J
J
Fraipont
C
C
Joris
B
B
Matagne
A
A
Dusart
J
J
D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D003001:Cloning, Molecular; D003062:Codon; D004269:DNA, Bacterial; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010406:Penicillins / Q000378:metabolism; D012045:Regulatory Sequences, Nucleic Acid; D017386:Sequence Homology, Amino Acid; D013302:Streptomyces / Q000201:enzymology / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D001618:beta-Lactamases / Q000235:genetics*; D047090:beta-Lactams
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D003062:Codon; D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; D010406:Penicillins; D014157:Transcription Factors; D047090:beta-Lactams; C071526:AmpR protein, Bacteria; D001618:beta-Lactamases