title: Nuclear localization of estrogen receptor-immunoreactivity in the preoptic area of female rats and its reduction by intraventricular colchicine treatment.
abstract: The subcellular localization of estrogen receptor (ER) was investigated in the preoptic area of ovariectomized female rats by electron microscopic immunohistochemistry, using a monoclonal antibody to ER. ER-immunoreactivity was localized in the nuclei of neurons of the periventricular preoptic nucleus (Pe) and the medial preoptic area (MPA). ER-immunoreactivity had a speckled pattern in the nucleus, but was not observed in the nucleolus or cytoplasm. After intraventricular colchicine treatment, ER-immunoreactivity within the nucleus was reduced drastically in neurons of the Pe and the MPA. The possible mechanism by which colchicine alters ER-immunoreactivity is mentioned.
authors.affiliation: Department of Anatomy, Kyoto Prefectural University of Medicine, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Yuri
authors.forename: M
authors.initials: M
authors.lastname: Kawata
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D001345:Autoradiography; D002467:Cell Nucleus / Q000378:metabolism*; D003078:Colchicine / Q000008:administration & dosage / Q000494:pharmacology*; D005260:Female; D007276:Injections, Intraventricular; D009474:Neurons / Q000378:metabolism / Q000648:ultrastructure; D010052:Ovariectomy; D011301:Preoptic Area / Q000276:immunology / Q000378:metabolism* / Q000648:ultrastructure; D051381:Rats; D017208:Rats, Wistar; D011960:Receptors, Estrogen / Q000276:immunology / Q000378:metabolism*
chemical_list: D000911:Antibodies, Monoclonal; D011960:Receptors, Estrogen; D003078:Colchicine