An integrated system for quantitation of chemotaxis using a 48-well millipore filter assay.
We have established a computerized system for quantification of human neutrophil motility using a 48-well chemotaxis assay. The software is primarily written in the Unix C-shell and is designed to integrate with standard statistical and graphics packages and permit analysis either under Unix or MS-DOS. We demonstrate how simple image analysis techniques may be used to count neutrophils that have traversed a polycarbonate filter. Methods of optical optimization, cell counting and integration with the Statistical Analysis System (SAS) are presented.
Coates
T D
TD
Department of Pediatrics, Childrens Hospital of Los Angeles, University of Southern California, School of Medicine 90027.
D002634:Chemotaxis, Leukocyte; D005374:Filtration; D006801:Humans; D007090:Image Interpretation, Computer-Assisted / Q000379:methods / Q000592:standards*; D007958:Leukocyte Count / Q000379:methods*; D008853:Microscopy; D008905:Minicomputers; D009504:Neutrophils; D012985:Software Design