[Quantitative determination of anthracycline antibiotic binding with DNA].
A new method of quantitative intravital assessment of anthracyclines accumulation and binding with DNA in alive cells has been developed. For this purpose DNA specific fluorescent dye Hoechst 33258 was used. Extent of fluorescence quenching of bound with DNA dye correlated with binding of daunomycin with cell DNA after mixing solutions of the drug and cells. It allowed us to determine quantity of drug molecules bound with DNA in the cells during the time.
Bogush
T A
TA
Baranov
E P
EP
Egudina
S V
SV
Tankovich
N N
NN
D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000378:metabolism*; D002845:Chromatography; D004247:DNA / Q000378:metabolism*; D004317:Doxorubicin / Q000378:metabolism; D005456:Fluorescent Dyes; D051379:Mice; D008810:Mice, Inbred C57BL
D000903:Antibiotics, Antineoplastic; D005456:Fluorescent Dyes; D004317:Doxorubicin; D004247:DNA
Kolichestvennoe opredelenie sviazyvaniia antratsiklinovykh antibiotikov s DNK.