Effect of gluco- and mineralocorticoids on gene expression of atrial natriuretic peptide by rat atria in vivo.
The effect of gluco- and mineralocorticoids on atrial natriuretic peptide (ANP) gene expression as well as plasma sodium and potassium concentrations was studied in both intact and adrenalectomized Wistar rats. Atria total RNA was extracted with cold phenol method and hybridized to alpha-32P labeled rat ANP-cDNA probe. The ANP gene transcripts in intact rats receiving dexamethasone and in adrenalectomized rats receiving both dexamethasone and deoxy-corticosterone increased by twofold, while there were no significant changes in the other groups. There were no parallel correlations between the rise of corticoid hormones-induced plasma Na+ concentration and the enhancement of rANP-mRNA level. The results suggest that glucocorticoids, when used together with mineralocorticoids, may directly increase ANP gene expression, whereas administration of mineralocorticoids alone has no effect on ANP gene expression.
Hong
M
M
Department of Biochemistry, Norman Bethune University of Medical Sciences, Changchun.
Liu
J K
JK
Bao
T
T
Yang
S N
SN
D000315:Adrenalectomy; D000818:Animals; D009320:Atrial Natriuretic Factor / Q000187:drug effects* / Q000235:genetics*; D003900:Desoxycorticosterone / Q000494:pharmacology*; D003907:Dexamethasone / Q000494:pharmacology*; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D011994:Recombinant Proteins; D012964:Sodium / Q000097:blood
D012333:RNA, Messenger; D011994:Recombinant Proteins; D003900:Desoxycorticosterone; D003907:Dexamethasone; D009320:Atrial Natriuretic Factor; D012964:Sodium