Use of sulphonated probes for detecting human immunodeficiency virus-1 transcripts by in situ hybridization.
A detailed procedure is described that allows detection of the presence of human immunodeficiency virus-1 (HIV-1) transcripts within both acetone-fixed tissues and peripheral blood mononuclear cells. This assay uses cDNA probes labelled by a non-isotopic procedure that results in the modification of cytosine residues through covalent linkage to a sulphone group. In situ hybridized probe is then detected by an alkaline phosphatase-conjugated antibody specifically directed against the sulphone hapten. This procedure is specific, rapid and safe and can be applied in the research as well as in the clinical pathology settings.
Pezzella
M
M
Institute of Infectious Diseases, University La Sapienza, Policlinico Umberto I, Rome, Italy.
Vonesch
N
N
Sturchio
E
E
D015497:HIV-1 / Q000235:genetics*; D006241:Haptens / Q000032:analysis; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D015345:Oligonucleotide Probes; D012367:RNA, Viral / Q000032:analysis*; D013450:Sulfones / Q000032:analysis
D006241:Haptens; D015345:Oligonucleotide Probes; D012367:RNA, Viral; D013450:Sulfones