title: Differential pituitary response to GnRH in pregnant Booroola-Assaf and Assaf ewes.
abstract: Pituitary response to exogenous gonadotrophin-releasing hormone (GnRH) was examined at a late stage of pregnancy in 10 Booroola-Assaf ewes heterozygous at the FecB locus (FecBFec+) and in 11 Assaf ewes that were non-carriers (Fec+Fec+). Basal plasma luteinizing hormone (LH) and follicle stimulating hormone (FSH) concentrations were similar in the two genotypes. Administration of 100 micrograms of GnRH resulted in a significant increase (P < 0.05) in plasma LH and FSH concentrations in most of the ewes treated. Maximal responses were observed 105-135 min after GnRH treatment. Pituitary responses to GnRH were significantly lower (P < 0.05) in Booroola-Assaf than in Assaf ewes. The decreased pituitary responsiveness observed in FecB gene carriers compared with Fec+Fec+ ewes might be due to differences in the concentrations of ovarian or uterine hormones modulating the release of gonadotrophin. The results suggest that FecB-specific differences can be observed at the pituitary level.
authors.affiliation: Institute of Animal Science, ARO, Volcani Center, Bet Dagan, Israel.
authors.forename: E
authors.initials: E
authors.lastname: Gootwine
authors.forename: A
authors.initials: A
authors.lastname: Bor
authors.forename: D
authors.initials: D
authors.lastname: Shalhevet
authors.forename: A
authors.initials: A
authors.lastname: Zenou
authors.forename: R
authors.initials: R
authors.lastname: Braw-Tal
mesh_terms: D000818:Animals; D005260:Female; D005640:Follicle Stimulating Hormone / Q000097:blood / Q000378:metabolism; D005838:Genotype; D007987:Gonadotropin-Releasing Hormone / Q000494:pharmacology*; D006579:Heterozygote; D007986:Luteinizing Hormone / Q000097:blood / Q000378:metabolism; D010902:Pituitary Gland / Q000187:drug effects* / Q000378:metabolism; D011247:Pregnancy; D012756:Sheep; D013045:Species Specificity
chemical_list: D007987:Gonadotropin-Releasing Hormone; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone