Drop-delay measurement using enzyme-coated particles.
A simple technique is suggested for the measurement of drop delay for flow sorting. While the flow cytometer was set to sort a fixed number of particles, the drop-delay setting was changed step by step, and at each step the HRP-coupled particles were sorted into a well of an immunoassay strip. Then the HRP activity of the sorted samples was revealed by routine methods. The maximum level of the enzyme activity shows the proper drop-delay setting. Determination of the drop-delay setting takes only a few minutes. The technique is independent of the type of flow cytometer and does not require any additional equipment.
Lazebnik
Y A
YA
Institute of Cytology, Academy of Sciences of the USSR, St. Petersburg.
Poletaev
A I
AI
Zenin
V V
VV
D000818:Animals; D001562:Benzimidazoles; D001772:Blood Cell Count / Q000295:instrumentation / Q000379:methods; D002469:Cell Separation / Q000295:instrumentation / Q000379:methods*; D005434:Flow Cytometry / Q000295:instrumentation / Q000379:methods*; D016650:Fluorescein-5-isothiocyanate; D006735:Horseradish Peroxidase; D008856:Microscopy, Fluorescence; D009391:Nephelometry and Turbidimetry; D011898:Ranidae / Q000097:blood; D013997:Time Factors
D001562:Benzimidazoles; D006735:Horseradish Peroxidase; D016650:Fluorescein-5-isothiocyanate; C017807:bisbenzimide ethoxide trihydrochloride