Metabolism in and function of the central dopamine system after transplanting adrenal medullary tissue into the rat brain.
The unilateral nigrostriatal pathway of rats was destroyed by microinjecting 6-hydroxydopamine (6-OH-DA), and isogenous rat adrenal medullary tissue was then transplanted into the lesioned lateral caudate nucleus. After 2 months, rotational movement was almost abolished in 40% of the transplanted rats, and it was significantly reduced in the remaining 60%. Dopamine (DA) and its metabolites contents were significantly decreased in the lesioned side of the forebrain, while no changes of NA were found. After transplanting adrenal medullary tissue, the DA contents increased by 9% only, whereas the NA contents increased significantly. In addition, by examining synaptosomes in the brains of these rats, we found that on the lesioned side of nontransplanted rats, DA uptake and DA receptor binding were increased significantly, while no such changes were seen in the transplanted group.
Zuo
P
P
Institute of Basic Medical Sciences, CAMS, Beijing.
Yu
C
C
Wu
R
R
Sui
J
J
D015102:3,4-Dihydroxyphenylacetic Acid / Q000378:metabolism; D000313:Adrenal Medulla / Q000637:transplantation*; D000818:Animals; D001921:Brain / Q000378:metabolism* / Q000601:surgery; D004298:Dopamine / Q000378:metabolism*; D005260:Female; D008297:Male; D009638:Norepinephrine / Q000378:metabolism; D016627:Oxidopamine / Q000494:pharmacology; D016548:Prosencephalon / Q000378:metabolism / Q000601:surgery; D051381:Rats; D017208:Rats, Wistar; D013378:Substantia Nigra / Q000187:drug effects
D015102:3,4-Dihydroxyphenylacetic Acid; D016627:Oxidopamine; D004298:Dopamine; D009638:Norepinephrine