Purification and biochemical properties of calmodulin in Entamoeba histolytica and its distribution during secretion of electron-dense granules.
1. Calmodulin (CaM) was detected during secretion of electron-dense granules by Entamoeba histolytica trophozoites with immunofluorescence. 2. It was purified to apparent homogeneity by chromatography with a yield of 2.26 micrograms of calmodulin/mg of protozoan protein. Purity was established by gel electrophoresis. 3. The parasite calmodulin has properties characteristic of calmodulin isolated from other eukaryotes: an apparent molecular weight of 19 or 17 kDa in presence of EGTA or CaCl2, respectively, activation in a calcium dependent manner of bovine heart cyclic nucleotide phosphodiesterase, and its UV spectrum.
Muńoz
M L
ML
Department of Genetics and Molecular Biology, Centro de Investigación y de Estudios Avanzados del I.P.N., San Pedro Zacatenco, México, D. F.
O'Shea-Alvarez
M S
MS
Pérez-García
J
J
Weinbach
E C
EC
Moreno
M A
MA
de la Torre
M
M
Magos
M A
MA
Tovar
R
R
D000818:Animals; D002147:Calmodulin / Q000302:isolation & purification / Q000378:metabolism*; D003594:Cytoplasmic Granules / Q000378:metabolism; D004748:Entamoeba histolytica / Q000378:metabolism* / Q000648:ultrastructure; D008856:Microscopy, Fluorescence; D008970:Molecular Weight; D013347:Subcellular Fractions / Q000378:metabolism
D002147:Calmodulin