Sphingosine inhibits factor Xa-catalyzed prothrombin activation on the surface of cultured calf pulmonary artery endothelium perturbed by hydrogen peroxide.
The effects of hydrogen peroxide on factor Xa-catalyzed prothrombin activation on the surface of vascular endothelial cells, and the inhibitory effect of sphingosine on prothrombin activation were studied in cultured calf pulmonary artery endothelial cells (CPAEC). Hydrogen peroxide enhanced factor Xa-catalyzed prothrombin activation on the cell surface in a time and dose-dependent manner. Sphingosine, a potent inhibitor of protein kinase C and the extrinsic pathway of blood coagulation, showed a partial inhibition of prothrombin activation on the surface of CPAEC. When cells were perturbed by hydrogen peroxide, sphingosine inhibited prothrombin activation and displaced factor Xa bound to the cell surface.
Tanabe
S
S
Tokyo Research Laboratories, Kowa Company, Ltd., Japan.
Tamaki
T
T
Wada
Y
Y
D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000378:metabolism; D004789:Enzyme Activation / Q000187:drug effects; D065427:Factor Xa Inhibitors; D006861:Hydrogen Peroxide / Q000633:toxicity*; D008969:Molecular Sequence Data; D010741:Phospholipases A / Q000037:antagonists & inhibitors; D011493:Protein Kinase C / Q000037:antagonists & inhibitors* / Q000502:physiology; D011516:Prothrombin / Q000378:metabolism*; D011651:Pulmonary Artery / Q000166:cytology; D013110:Sphingosine / Q000494:pharmacology*
D065427:Factor Xa Inhibitors; D011516:Prothrombin; D006861:Hydrogen Peroxide; D011493:Protein Kinase C; D010741:Phospholipases A; D013110:Sphingosine