title: Properties of HIV membrane reconstituted from its recombinant gp160 envelope glycoprotein.
abstract: Human immunodeficiency virus (HIV) membrane has been reconstituted from the recombinant envelope glycoprotein precursor (gp160) by a detergent dialysis technique. Electron microscopy shows that gp160-virosomes are spherical vesicles with a mean diameter identical to that of viral particles. Enzyme-linked immunosorbent assay and immunogold labeling demonstrate efficient association of gp160 with lipid vesicles and proteolysis treatment reveals an asymmetric insertion with about 90% of glycoproteins having their gp120-moiety pointing outside. Glycoproteins are organized as dimers and tetramers and gp160 retains its ability to specifically bind CD4 receptor after reconstitution into virosome.
authors.affiliation: Laboratoire de Chimie-Physique des Macromolécules aux Interfaces, Université Libre de Bruxelles, Belgium.
authors.forename: B
authors.initials: B
authors.lastname: Cornet
authors.forename: E
authors.initials: E
authors.lastname: Decroly
authors.forename: D
authors.initials: D
authors.lastname: Thines-Sempoux
authors.forename: J M
authors.initials: JM
authors.lastname: Ruysschaert
authors.forename: M
authors.initials: M
authors.lastname: Vandenbranden
mesh_terms: D015704:CD4 Antigens / Q000378:metabolism; D015686:Gene Products, env / Q000737:chemistry / Q000378:metabolism*; D019302:HIV Envelope Protein gp160; D015497:HIV-1 / Q000378:metabolism* / Q000648:ultrastructure*; D006801:Humans; D066298:In Vitro Techniques; D008081:Liposomes; D016253:Microscopy, Immunoelectron; D011487:Protein Conformation; D011498:Protein Precursors / Q000737:chemistry / Q000378:metabolism*; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism
chemical_list: D015704:CD4 Antigens; D015686:Gene Products, env; D019302:HIV Envelope Protein gp160; D008081:Liposomes; D011498:Protein Precursors; D011994:Recombinant Proteins