Temperature-sensitive mutants of the Mycobacterium plasmid pAL5000.
Two plasmids were isolated as thermosensitive replicons following in vitro mutagenesis of pB4, a pAL5000 derivative mycobacteria/Escherichia coli shuttle plasmid. Plasmids pCG59 and pCG63 replicate at 30 degrees C but not at 39 degrees C. This will allow their utilisation for transposon delivery, site-specific integration, or allele exchange.
Guilhot
C
C
Unité de Génie Microbiologique, URA1300 du CNRS, Institut Pasteur, Paris, France.
Gicquel
B
B
Martín
C
C
D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial; D005822:Genetic Vectors; D016296:Mutagenesis; D009161:Mycobacterium / Q000235:genetics*; D010957:Plasmids; D012093:Replicon; D013696:Temperature; D014170:Transformation, Genetic