Derivation of a physical map of the chromosome of Bordetella pertussis Tohama I.
We have used pulsed-field gel electrophoresis to derive a restriction map of the chromosome of Bordetella pertussis for the enzymes XbaI, SpeI, PacI, and PmeI, which cleave 25, 16, 2, and 1 times, respectively. The apparent size of the genome is 3,750 kb. The positions of genes for major virulence determinants in the vir regulon and of some housekeeping genes were determined. Apart from the previously known linkage of the vir and fha loci, no significant linkage of virulence genes was demonstrated.
Stibitz
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Laboratory of Bacterial Toxins, Food and Drug Administration, Bethesda, Maryland 20892.
Garletts
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D001483:Base Sequence; D001886:Bordetella pertussis / Q000235:genetics*; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D004269:DNA, Bacterial / Q000235:genetics; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D016521:Electrophoresis, Gel, Pulsed-Field; D005798:Genes, Bacterial / Q000235:genetics; D008969:Molecular Sequence Data
D004269:DNA, Bacterial; C078388:endodeoxyribonuclease PmeI; C056436:endodeoxyribonuclease SpeI; C025580:endodeoxyribonuclease XBAI; D015252:Deoxyribonucleases, Type II Site-Specific; C068375:endodeoxyribonuclease PacI