Polyclonal antibody localizes glia maturation factor beta-like immunoreactivity in neurons and glia.
A rabbit polyclonal antibody (91-01) was raised against recombinant human glia maturation factor beta (r-hGMF-beta). The antibody did not cross-react with a number of other growth factors on ELISA test. When compared with the monoclonal antibody G2-09 previously obtained, 91-01 immunoblotted the same protein band in rat brain extract. However, unlike G2-09 which immunostained only astrocytes and Bergmann glia, 91-01 stained neurons as well. Many but not all neurons in the central and peripheral nervous system were positive for GMF-beta. The larger cell population stained by the polyclonal antibody was most likely due to its increased sensitivity, although other explanations are possible. The presence of GMF-beta-like immunoreactivity in both neurons and glia raises the possibility of a wider range of cell-cell interaction than was previously considered.
Wang
B R
BR
Department of Neurology, University of Iowa College of Medicine, Iowa City 52242.
Zaheer
A
A
Lim
R
R
D000818:Animals; D000906:Antibodies / Q000276:immunology*; D000918:Antibody Specificity / Q000276:immunology; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D020904:Glia Maturation Factor; D006131:Growth Inhibitors / Q000032:analysis*; D007150:Immunohistochemistry; D007163:Immunosorbent Techniques; D009419:Nerve Tissue Proteins / Q000032:analysis*; D009457:Neuroglia / Q000737:chemistry*; D009474:Neurons / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011994:Recombinant Proteins / Q000032:analysis
D000906:Antibodies; D020904:Glia Maturation Factor; D006131:Growth Inhibitors; D009419:Nerve Tissue Proteins; D011994:Recombinant Proteins