title: Analytical and micropreparative separation of peptides by capillary zone electrophoresis using discontinuous buffer systems.
abstract: The tiny injection volumes that are usually necessary to maintain the high efficiency of capillary zone electrophoresis present a major problem if only limited sample amounts are available. To increase the sample load, discontinuous buffer systems were developed that allow the on-column concentration of dilute samples. Injection volumes can be increased in this way by at least a factor of 30. These stacking systems were applied to the analysis of tryptic peptides, to the purity checking of high-performance liquid chromatographic fractions and for the micropreparative separation of peptides with subsequent amino acid sequence analysis.
authors.affiliation: Department of Protein Chemistry, Max-Planck-Institute for Biochemistry, Martinsried, Germany.
authors.forename: C
authors.initials: C
authors.lastname: Schwer
authors.forename: F
authors.initials: F
authors.lastname: Lottspeich
mesh_terms: D000595:Amino Acid Sequence; D002021:Buffers; D002851:Chromatography, High Pressure Liquid; D004586:Electrophoresis / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D010455:Peptides / Q000302:isolation & purification*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D002021:Buffers; D010455:Peptides