The stereochemical course of sulphuryl transfer catalysed by arylsulphatase II from Aspergillus oryzae.
Phenyl [(R)-16O,17O,18O]sulphate was synthesized and used to study the stereochemical course of sulphuryl transfer to p-cresol catalysed by arylsulphatase II from Aspergillus oryzae. The reaction was shown to proceed with retention of configuration at the sulphur atom, providing evidence for the involvement of a sulpho-enzyme intermediate on the reaction pathway.
Chai
C L
CL
Dyson Perrins Laboratory, University of Oxford, U.K.
Loughlin
W A
WA
Lowe
G
G
D001192:Arylsulfatases / Q000737:chemistry / Q000378:metabolism*; D001236:Aspergillus oryzae / Q000201:enzymology*; D003408:Cresols / Q000378:metabolism; D005583:Fourier Analysis; D009682:Magnetic Resonance Spectroscopy; D008968:Molecular Conformation; D013055:Spectrophotometry, Infrared; D013455:Sulfur / Q000378:metabolism*
D003408:Cresols; C032538:4-cresol; D013455:Sulfur; D001192:Arylsulfatases