title: [Escherichia coli virulence factors causing peritonitis, appendicitis and other extraintestinal infections].
abstract: Fifty-nine E. coli strains isolated from clinical cases of peritonitis, appendicitis, cholecystitis, wounds and respiratory infections as well as from other miscellaneous sources were investigated. A control group constituted by 475 E. coli strains isolated from the faeces of healthy individuals were also studied. E. coli O-grouped and investigated for production of cytotoxic necrotizing factor CNF1 and alpha-haemolysin (Hly), expression of P fimbriae and mannose-resistant (MRHA) and mannose-sensitive (MSHA) haemagglutination. Virulence factors significantly associated with extraintestinal strains were: production of CNF1 (19% versus 5%, p < 0.001), Hly (27% versus 9%; p < 0.001) and expression of MRHA (44% versus 16%; p < 0.001). The majority of extraintestinal strains (68% versus 36%; p < 0.001), in contrast with faecal E. coli, belonged to O serogroups frequently detected in uropathogenic and bacteraemic E. coli. These results suggest that E. coli causing different types of extraintestinal infections show similar virulence factors and belong to the same serogroups. However, between E. coli isolated from intraabdominal, wound and respiratory infections the number of strains with virulence factors was lower than in E. coli causing urinary tract infections and sepsis.
authors.affiliation: Departamento de Microbiología y Parasitología, Facultad de Veterinaria, Universidad de Santiago, Lugo.
authors.forename: J
authors.initials: J
authors.lastname: Blanco
authors.forename: J E
authors.initials: JE
authors.lastname: Blanco
authors.forename: M P
authors.initials: MP
authors.lastname: Alonso
authors.forename: M
authors.initials: M
authors.lastname: Blanco
authors.forename: J I
authors.initials: JI
authors.lastname: Garabal
authors.forename: E A
authors.initials: EA
authors.lastname: González
mesh_terms: D000818:Animals; D001064:Appendicitis / Q000382:microbiology*; D002764:Cholecystitis / Q000382:microbiology; D004926:Escherichia coli / Q000145:classification / Q000302:isolation & purification / Q000472:pathogenicity*; D004927:Escherichia coli Infections / Q000382:microbiology*; D005243:Feces / Q000382:microbiology; D006801:Humans; D010538:Peritonitis / Q000382:microbiology*; D012141:Respiratory Tract Infections / Q000382:microbiology; D014774:Virulence; D014947:Wounds and Injuries / Q000382:microbiology
vernacular_title: Factores de virulencia de Escherichia coli causantes de peritonitis, apendicitis y otras infecciones extraintestinales.