title: Relative bioavailability of lead from mining waste soil in rats.
abstract: The purposes of this study were to determine the extent of absorption of lead (Pb) in mining waste soil from Butte, Montana, and to investigate the effect of mining waste soil dose (g soil/day) on tissue lead concentrations. Young, 7- to 8-week-old male and female Sprague-Dawley rats (5/sex/group) were given mining waste soil that contained 810 or 3908 ppm lead mixed in a purified diet (AIN-76) at four different dose levels (0.2, 0.5, 2, and 5% dietary soil) for 30 consecutive days. Standard groups included untreated controls and dosed feed soluble lead acetate groups (1, 10, 25, 100, and 250 micrograms Pb/g feed). The test soil dose levels bracketed a pica child's soil exposure level and the lead acetate concentrations bracketed the test soil dose levels of lead. Liver, blood, and femur were analyzed for total lead concentration using graphite furnace atomic absorption spectroscopy. Clinical signs, body weight, food consumption, and liver weights for test soil and standard groups were similar to control. Tissue lead concentrations from test soil animals were significantly lower than the tissue concentrations for the lead acetate group. Relative percentage bioavailability values, based on lead acetate as the standard, were independent of the two different test soils, dose levels, and sex and were only slightly dependent on the tissue (blood > bone, liver). Mean relative percentage bioavailability values of lead in the Butte mining waste soil were 20% based on the blood data, 9% based on the bone data, and 8% based on the liver data. The results of this study will provide the information needed to determine the significance of lead exposure from Butte soils in assessing human health risks as part of the Superfund Remedial Investigation/Feasibility Study process.
authors.affiliation: Battelle, Columbus, Ohio 43201.
authors.forename: G B
authors.initials: GB
authors.lastname: Freeman
authors.forename: J D
authors.initials: JD
authors.lastname: Johnson
authors.forename: J M
authors.initials: JM
authors.lastname: Killinger
authors.forename: S C
authors.initials: SC
authors.lastname: Liao
authors.forename: P I
authors.initials: PI
authors.lastname: Feder
authors.forename: A O
authors.initials: AO
authors.lastname: Davis
authors.forename: M V
authors.initials: MV
authors.lastname: Ruby
authors.forename: R L
authors.initials: RL
authors.lastname: Chaney
authors.forename: S C
authors.initials: SC
authors.lastname: Lovre
authors.forename: P D
authors.initials: PD
authors.lastname: Bergstrom
mesh_terms: D000818:Animals; D001682:Biological Availability; D001835:Body Weight / Q000187:drug effects; D004435:Eating / Q000187:drug effects; D005260:Female; D007220:Industrial Waste / Q000032:analysis*; D007854:Lead / Q000737:chemistry / Q000493:pharmacokinetics*; D008297:Male; D008906:Mining; D009009:Montana; D010316:Particle Size; D051381:Rats; D017207:Rats, Sprague-Dawley; D012307:Risk Factors; D012989:Soil Pollutants / Q000032:analysis*; D013054:Spectrophotometry, Atomic; D014018:Tissue Distribution
chemical_list: D007220:Industrial Waste; D012989:Soil Pollutants; D007854:Lead