Lack of effect of parathyroid hormone on calcium homeostasis in rat platelets.
The in vitro effects of parathyroid hormone on Ca2+ handling by fura-2 loaded rat platelets were studied. The incubation of these platelets with rat parathyroid hormone (1-34) for 10 min had no effect on intracellular fura-2 metabolism or [Ca2+]i in the resting state. The [Ca2+]i response to 0.1 U/mL thrombin was unaffected by preincubation with parathyroid hormone in the presence or absence of extracellular Ca2+. Furthermore, the recovery rate of [Ca2+]i after the thrombin-induced peak in Ca(2+)-depleted media was not altered with parathyroid hormone. These data indicate that parathyroid hormone may not have a significant effect on Ca2+ homeostasis in rat platelets in unstimulated and stimulated conditions.
Oshima
T
T
Department of Clinical Laboratory, Hiroshima University School of Medicine, Japan.
Tsubokura
T
T
Matsuura
H
H
Kainouchi
M
M
Kajiyama
G
G
McCarron
D A
DA
D000818:Animals; D001792:Blood Platelets / Q000378:metabolism*; D002118:Calcium / Q000097:blood*; D005110:Extracellular Space / Q000378:metabolism; D016257:Fura-2; D006706:Homeostasis; D007425:Intracellular Membranes / Q000378:metabolism; D008297:Male; D010281:Parathyroid Hormone / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
D010281:Parathyroid Hormone; D002118:Calcium; D016257:Fura-2