title: Epidermal growth factor (EGF) stimulation of ATP citrate lyase activity in isolated rat hepatocytes is age dependent.
abstract: 1. The effect of epidermal growth factor (EGF) on ATP citrate lyase activity was determined in freshly isolated hepatocytes from rats of different ages as a function of incubation time, EGF concentration and hepatocyte density. 2. The activity of this enzyme was responsive to both dose and time of incubation of EGF with a two-fold increase in ATP citrate lyase activity and a half-maximal effect between 10(-12) and 10(-11) M. 3. EGF effects were detectable by 5 min. 4. The age of the rats had a strong effect on the magnitude of the EGF effect with ATP citrate lyase activity in younger (8 weeks) rats being more responsive than in older (14 weeks) rats.
authors.affiliation: Department of Food Science and Technology, The Ohio State University, Columbus 43210.
authors.forename: K
authors.initials: K
authors.lastname: Emmerson
authors.forename: K
authors.initials: K
authors.lastname: Roehrig
mesh_terms: D001275:ATP Citrate (pro-S)-Lyase / Q000378:metabolism*; D000375:Aging / Q000378:metabolism; D000818:Animals; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation / Q000187:drug effects; D004815:Epidermal Growth Factor / Q000008:administration & dosage / Q000494:pharmacology*; D066298:In Vitro Techniques; D008099:Liver / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D004815:Epidermal Growth Factor; D001275:ATP Citrate (pro-S)-Lyase