title: Antibodies against somatic antigens and excreted/secreted products of Brugia pahangi in rats with patent and non-patent infections.
abstract: The humoral responses of Sprague-Dawley rats infected with Brugia pahangi were examined for up to 6 months after infection by ELISA, immunoblotting, and IFAT. In 2 experiments, 50% and 62.5% of rats developed patent, microfilaraemic infections. Mean adult worm burdens at autopsy were approximately 2% of the inoculum, and only patent rats yielded living adult worms. IgG antibody levels against crude somatic extracts (CSE) of all parasite stages and against adult excreted/secreted (ES) products were significantly higher in patent than non-patent rats. Both patent and non-patent rats produced anti-microfilarial surface antibody, as revealed by immunofluorescence. Immunostaining of Western blots by early infection sera showed no consistent difference in recognition of infective larval (L3) antigenic components by IgG or IgM antibody between eventually-patent and eventually-non-patent rats. By 26 weeks, however, patent rats recognized more components. The data suggest that antibodies against L3, adult, and microfilarial somatic antigens, ES antigens and microfilarial surface antigens do not correlate with the subsequent development of microfilaraemia in any individual rat.
authors.affiliation: Department of Parasitology, National Yang-Ming Medical College, Taipei, Taiwan, R.O.C.
authors.forename: C
authors.initials: C
authors.lastname: Fletcher
authors.forename: C C
authors.initials: CC
authors.lastname: Wu
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000096:biosynthesis* / Q000097:blood; D000947:Antigens, Helminth / Q000276:immunology*; D000954:Antigens, Surface / Q000276:immunology; D015153:Blotting, Western; D017179:Brugia pahangi / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005368:Filariasis / Q000097:blood / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000097:blood; D007814:Larva / Q000276:immunology; D008297:Male; D008842:Microfilariae / Q000276:immunology; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D000954:Antigens, Surface; D007074:Immunoglobulin G