Cellular immune responses in the skin of sheep infected with larvae of Lucilia cuprina, the sheep blowfly.
Cellular immune responses were observed in the skin of sheep after primary and secondary infection with sheep blowfly (Lucilia cuprina) larvae. Both primary and secondary infections resulted in a massive cellular infiltration within 48 h of wound initiation, with the majority of cells having the CD45 phenotype. Neutrophils comprised the major cell type at the skin surface. In the dermis, the number of CD4+ T helper, gamma delta-TCR+ cells and T19+ (CD4-, CD8-) T cells also increased significantly in skin during both primary and secondary infections compared with control sites. However, there was no significant difference in the numbers of these cells between primary and secondary infections. An increase in the expression of the CD1 antigen on Langerhans/dendritic cells was observed, along with an apparent increase in the number of these cells in secondary lesions, with the majority of the cells being concentrated in the upper dermis and epidermis. While there was no increase in mast cells, eosinophils increased significantly during infection compared with control sites. The cellular infiltration observed following primary and secondary infections suggests polyclonal activation of T cells and their selective recruitment to the lesion site.
Bowles
V M
VM
Centre for Animal Biotechnology, School of Veterinary Science, University of Melbourne, Parkville, Vic., Australia.
Grey
S T
ST
Brandon
M R
MR
D000818:Animals; D004175:Diptera / Q000276:immunology*; D004804:Eosinophils / Q000276:immunology; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D007111:Immunity, Cellular; D007814:Larva / Q000276:immunology; D016131:Lymphocyte Subsets / Q000276:immunology; D008297:Male; D008407:Mast Cells / Q000276:immunology; D009198:Myiasis / Q000276:immunology / Q000662:veterinary*; D009504:Neutrophils / Q000276:immunology; D010641:Phenotype; D012756:Sheep; D012757:Sheep Diseases / Q000276:immunology*; D012867:Skin / Q000276:immunology*
D000949:Histocompatibility Antigens Class II