Determination of ascorbic acid in pharmaceuticals and urine by reverse flow injection.
Two reverse flow injection (FI) methods, using spectrophotometric detection, are proposed for the determination of ascorbic acid. Both methods are based on its reaction with the ethylenediaminetetraacetic acid-CoIII complex in a medium of 5% diethylamine. In the first method, using the peak-height FI technique, ascorbic acid is determined over the range from 2 x 10(-4) to 5 x 10(-3) mol dm-3 and in the second, using the peak-width FI method, the working range is extended (2 x 10(-3)-5 x 10(-2) mol dm-3). Both FI methods were applied to the determination of ascorbic acid in pharmaceuticals while the peak-height FI technique was also used to determine ascorbic acid in urine.
Albero
M I
MI
Department of Analytical Chemistry, Faculty of Chemistry, University of Murcia, Spain.
Soledad García
M
M
Sánchez-Pedreño
C
C
Rodríguez
J
J
D001205:Ascorbic Acid / Q000032:analysis* / Q000652:urine; D002614:Chelating Agents; D003035:Cobalt; D004492:Edetic Acid; D006801:Humans; D007202:Indicators and Reagents; D013053:Spectrophotometry / Q000295:instrumentation / Q000379:methods
D002614:Chelating Agents; D007202:Indicators and Reagents; C028222:cobalt-ethylenediamine tetraacetic acid chelate; D003035:Cobalt; D004492:Edetic Acid; D001205:Ascorbic Acid