Expression of bacterial cytotoxin genes in mammalian target cells.
We have studied the expression of the gene fragments encoding the enzymatically active portion of three bacterial cytotoxins: exotoxin A (ETA) of Pseudomonas aeruginosa, and pertussis toxin (PT) and adenylate cyclase toxin (CYA) of Bordetella pertussis, in sensitive mammalian target cells. Expression of active ETA and CYA was lethal to the producing cells and stable transfectants of Cos-1 cells containing the corresponding genes could not be obtained. The expression of the PTS1 subunit was tolerated by the producing mammalian cells. Since PT is cytotoxic because of ADP-ribosylation of G-proteins, we assume that the endogenously expressed PTS1 may not find the cellular target G proteins or PTS1 alone may not be sufficient for ADP-ribosylation of these proteins in vivo.
Wels
W
W
Theodor-Boveri-Institut für Biowissenschaften (Biozentrum), Lehrstuhl für Mikrobiologie, Universität Würzburg, Germany.
Baldrich
M
M
Chakraborty
T
T
Gross
R
R
Goebel
W
W
D036002:ADP Ribose Transferases; D037361:Adenylate Cyclase Toxin; D000595:Amino Acid Sequence; D000818:Animals; D001427:Bacterial Toxins; D001483:Base Sequence; D001886:Bordetella pertussis / Q000235:genetics; D016923:Cell Death / Q000187:drug effects; D002460:Cell Line; D002522:Chlorocebus aethiops; D005098:Exotoxins / Q000096:biosynthesis* / Q000235:genetics / Q000633:toxicity; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism*; D015870:Gene Expression; D007739:L Cells / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000096:biosynthesis / Q000235:genetics / Q000633:toxicity; D037342:Pertussis Toxin; D011065:Poly(ADP-ribose) Polymerases / Q000096:biosynthesis* / Q000235:genetics / Q000633:toxicity; D011550:Pseudomonas aeruginosa / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis* / Q000633:toxicity; D037521:Virulence Factors; D010566:Virulence Factors, Bordetella / Q000096:biosynthesis* / Q000235:genetics / Q000633:toxicity; D000097668:Pseudomonas aeruginosa Exotoxin A
D036002:ADP Ribose Transferases; D037361:Adenylate Cyclase Toxin; D001427:Bacterial Toxins; D005098:Exotoxins; D010446:Peptide Fragments; D037342:Pertussis Toxin; D011065:Poly(ADP-ribose) Polymerases; D011993:Recombinant Fusion Proteins; D037521:Virulence Factors; D010566:Virulence Factors, Bordetella; D000097668:Pseudomonas aeruginosa Exotoxin A