title: Dual compartment (bicameral) culture: role of basement membrane in epithelial differentiation.
abstract: A number of years ago we reported that tight junctions between adjacent Sertoli cells subdivide the seminiferous epithelium into two compartments, basal and adluminal, thus forming the morphological basis of the blood-testis barrier. It is now generally believed that the special milieu created by the Sertoli cells in the adluminal compartment is essential for germ cell differentiation. In order to duplicate the compartmentalization that occurs in vivo, Sertoli cells were cultured in bicameral chambers on Millipore filters impregnated with a reconstituted basement membrane. Confluent monolayers of these cells were tall columnar (40-60 microns in height) and highly polarized. These Sertoli cell monolayers established electrical resistance that peaked when the Sertoli-Sertoli tight junctions developed in culture. In addition, the monolayers formed a permeability barrier to 3H-inulin and lanthanum nitrate. The bicameral chambers were utilized in a number of studies on protein secretion, and it was revealed that numerous proteins are secreted in a polarized manner. In another study, hormone-stimulated aromatase activity was measured in Sertoli cells grown on plastic culture dishes, plastic dishes coated with laminin or Matrigel, and in the bicameral chambers. Cell culture on basement membrane substrates decreased the FSH-dependent estrogen production. No estrogen production was observed when the Sertoli cells were cultured in the bicameral chambers. These results are in accord with the hypothesis that differentiated Sertoli cells lose their ability to metabolize androgen to estrogen in an hormone-dependent manner, whereas undifferentiated cells in culture, or in vivo, have a very active FSH-dependent aromatase activity.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Anatomy and Cell Biology, Georgetown University Medical Center, Washington, DC 20007.
authors.forename: M
authors.initials: M
authors.lastname: Dym
authors.forename: V
authors.initials: V
authors.lastname: Papadopoulos
mesh_terms: D000818:Animals; D001141:Aromatase / Q000378:metabolism; D001485:Basement Membrane / Q000502:physiology; D002454:Cell Differentiation / Q000502:physiology; D015194:Diffusion Chambers, Culture; D005109:Extracellular Matrix / Q000502:physiology; D008297:Male; D011506:Proteins / Q000378:metabolism; D012670:Seminiferous Epithelium / Q000166:cytology*
chemical_list: D011506:Proteins; D001141:Aromatase