title: Efficient selection of phleomycin-resistant Saccharomyces cerevisiae transformants.
abstract: The recently described dominant yeast marker Tn5ble confers phleomycin resistance on the yeast Saccharomyces cerevisiae (Gatignol, Baron and Tiraby, 1987. Mol. Gen. Genet. 207, 342-348). Incubation in non-selective medium prior to selection is critical, however, for getting phleomycin-resistant transformants. A 6-h incubation period was found to give optimal transformation frequencies, up to 10(5) transformants/micrograms plasmid, comparable to selection for uracil prototrophy (Ura+).
authors.affiliation: Department of Cell Biology and Genetics, Leiden University, The Netherlands.
authors.forename: T J
authors.initials: TJ
authors.lastname: Wenzel
authors.forename: A
authors.initials: A
authors.lastname: Migliazza
authors.forename: H Y
authors.initials: HY
authors.lastname: Steensma
authors.forename: J A
authors.initials: JA
authors.lastname: van den Berg
mesh_terms: D004352:Drug Resistance, Microbial; D010692:Phleomycins / Q000494:pharmacology*; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics*; D012641:Selection, Genetic; D014170:Transformation, Genetic
chemical_list: D010692:Phleomycins