Interaction of dopamine-beta-monooxygenase with chromaffin granule membrane lipids.
The interaction between bovine adrenal medullary dopamine-beta-monooxygenase and liposomes from chromaffin granule membrane lipids as a function of pH, lipid and salt concentration was studied by ultracentrifugation. Efficient adsorption of dopamine-beta-monooxygenase to liposomes occurs in the pH range 5.0-6.5 and at low ionic strength. The adsorption was not detected in the case of apoenzyme. The membrane dopamine-beta-monooxygenase forms a complex with liposomes more effective than soluble does. The data obtained lead to certain conclusions about the specificity of complex between the enzyme and liposomes.
Pogosyan
A G
AG
Institute of Molecular Biology, Academy of Sciences of the Armenian Republic, Yerevan.
Boyajian
A S
AS
Mkrtchyan
M Y
MY
Karagezyan
K G
KG
D000313:Adrenal Medulla / Q000201:enzymology / Q000378:metabolism*; D000327:Adsorption; D000818:Animals; D002417:Cattle; D002837:Chromaffin Granules / Q000201:enzymology / Q000378:metabolism*; D004299:Dopamine beta-Hydroxylase / Q000378:metabolism*; D007700:Kinetics; D008081:Liposomes; D008563:Membrane Lipids / Q000378:metabolism*; D009994:Osmolar Concentration; D011485:Protein Binding
D008081:Liposomes; D008563:Membrane Lipids; D004299:Dopamine beta-Hydroxylase