A 1H-NMR study of the interactions between rat tissue kallikrein and two peptide inhibitors.
The 1H-NMR spectra have been obtained for rat submandibular kallikrein in the absence and presence of inhibitors. Two competitive inhibitors were investigated, the tripeptide leupeptin (a potent inhibitor with Ki 0.5 microM) and a hexapeptide (a much weaker, substrate-analogue inhibitor with Ki 380 microM). Analysis of the NMR spectra showed that binding of leupeptin to kallikrein led to a change in the conformation of the enzyme, whereas binding of the substrate analogue to the enzyme produced no such change and may have resulted in a conformational change of the inhibitor.
el-Thaher
T S
TS
Department of Chemistry and Biological Chemistry, University of Essex, Colchester, UK.
Bailey
G S
GS
Wilson
M T
MT
Osborne
M
M
Moore
G R
GR
D000595:Amino Acid Sequence; D000818:Animals; D007610:Kallikreins / Q000037:antagonists & inhibitors*; D007976:Leupeptins / Q000494:pharmacology*; D009682:Magnetic Resonance Spectroscopy; D008968:Molecular Conformation; D008969:Molecular Sequence Data; D011487:Protein Conformation; D051381:Rats; D013363:Submandibular Gland / Q000201:enzymology*
D007976:Leupeptins; D007610:Kallikreins; C032854:leupeptin