Inactivation of lambda phage with 658 nm light using a DNA binding porphyrin sensitizer.
Exposure of lambda phage to 658 nm light in the presence of 5,10,15,20-tetrakis-(1-methyl-4-pyridyl)-21H,23H-porphine, tetra-p-tosylate leads to complete (greater than 7 logs) inactivation as measured by the plaque assay. The sensitizer without light and 658 nm photolysis of lambda phage in the absence of sensitizer do not lead to a measurable decrease in viral inactivity. Viral inactivation is not dependent upon the presence of oxygen.
Kasturi
C
C
Department of Chemistry, Ohio State University, Columbus 43210.
Platz
M S
MS
D010582:Bacteriophage lambda / Q000187:drug effects / Q000528:radiation effects*; D008027:Light; D010782:Photolysis; D010957:Plasmids / Q000187:drug effects / Q000528:radiation effects*; D011166:Porphyrins / Q000494:pharmacology*; D011838:Radiation-Sensitizing Agents / Q000494:pharmacology*; D013329:Structure-Activity Relationship
D011166:Porphyrins; D011838:Radiation-Sensitizing Agents; C021096:tetra(4-N-methylpyridyl)porphine