Analysis of L-alanine:2-oxoglutarate aminotransferase isozymes in maize.
Isozyme analysis of L-alanine:2-oxoglutarate aminotransferase (ALT) in maize indicates that there are three genes encoding this enzyme activity. Two of the gene products interact with each other to form heterodimers, while the third gene product does not interact with the other two. Another isozyme that appears after gel electrophoresis and ALT staining is shown to be glutamate dehydrogenase-1. Anaerobic treatment does not result in increased ALT levels, indicating that the previously reported increase in alanine levels caused by this treatment may be due to increases in the level of pyruvate, a substrate of ALT.
Watson
N R
NR
Department of Biology, Washington University, St. Louis, Missouri 63130.
Peschke
V M
VM
Russell
D A
DA
Sachs
M M
MM
D000410:Alanine Transaminase / Q000032:analysis* / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D005786:Gene Expression Regulation; D007527:Isoenzymes / Q000032:analysis* / Q000235:genetics; D010100:Oxygen / Q000502:physiology; D003313:Zea mays / Q000201:enzymology* / Q000235:genetics
D007527:Isoenzymes; D000410:Alanine Transaminase; D010100:Oxygen