Cartilage canal growth: experimental approach in the rat tibia.
This paper studies the influence of an antimitotic factor (puromycin) and a hormonal factor (thyroid hormone, TH) on canal growth. The tibiae of 15 six-day-old rats were cultured in a serum-free chemically defined medium. The cultures were carried out for as long as 6 days. Our results show: (1) canal growth is not dependent on perichondrium or chondro-epiphysis growth; (2) the canal is greater and has a complex pattern in a triiodothyronine (T3)-treated assay group; (3) round and multinucleated cells are more numerous in the T3-treated assay group than in the other groups. We hypothesize that the canal grows by a physiological phenomenon of programmed cell death and that it is stimulated by TH.
Delgado-Baeza
E
E
Laboratorio de Microcirugía Experimental, Hospital La Paz, Madrid, España.
Nieto-Chaguaceda
A
A
Miralles-Flores
C
C
Santos-Alvarez
I
I
D000818:Animals; D006132:Growth Plate / Q000033:anatomy & histology / Q000187:drug effects / Q000254:growth & development*; D011691:Puromycin / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013977:Tibia / Q000033:anatomy & histology / Q000187:drug effects / Q000254:growth & development*; D014284:Triiodothyronine / Q000494:pharmacology*
D014284:Triiodothyronine; D011691:Puromycin