title: Identification of peaks in capillary zone electrophoresis based on actual mobilities.
abstract: A procedure is proposed for the calculation of the actual effective mobility of a zone from its migration time. It is based on the use of internal standards with known mobilities; the use of two internal standards provides reliable mobility data even if the magnitudes of the effects of sample composition, capillary temperature, capillary length, migration distance, used voltage, as well as the tube length occupied by the injected sample are unknown. Formulas have been derived for the calculation of the actual mobilities, and their experimental verification has been carried out by using a model set of anionic solutes with mobilities ranging from -56 to -20 x 10(-9) m2V-1s-1 and chloride as the ion modelling the effect of the sample matrix.
authors.affiliation: Institute of Analytical Chemistry, Czechoslovak Academy of Sciences, Brno.
authors.forename: R
authors.initials: R
authors.lastname: Vespalec
authors.forename: P
authors.initials: P
authors.lastname: Gebauer
authors.forename: P
authors.initials: P
authors.lastname: Bocek
mesh_terms: D004573:Electrolytes; D004586:Electrophoresis / Q000379:methods*; D008956:Models, Chemical; D009995:Osmosis; D012965:Sodium Chloride; D013056:Spectrophotometry, Ultraviolet; D013696:Temperature
chemical_list: D004573:Electrolytes; D012965:Sodium Chloride