Pituitary factors in blood plasma are necessary for smooth muscle cell proliferation in response to injury in vivo.
Intimal thickening in response to vascular injury is inhibited in animals previously subjected to hypophysectomy. We have investigated the nature and cell kinetics of this effect in a balloon catheter model of injury to the rat carotid artery. The ability of injury to stimulate [3H]thymidine labeling 48 hours after injury was almost completely eliminated in hypophysectomized (hypox) compared with control animals (0.1% versus 32.1%). Total DNA content of the developing neointima 14 days after injury was only 30% of the values found in ballooned carotid arteries of normal rats. If hypox rats were treated with recombinant human growth hormone, the proliferative response was not restored. There are two possible general explanations for the reduction of proliferative response in hypox animals: 1) that smooth muscle cells in the hypox animals have lost the ability to respond to the stimulus of injury or 2) that the ability of the smooth muscle cells to respond has not been reduced by prior hypophysectomy, but that the response itself requires the presence of pituitary-dependent factors. Transplantation experiments were performed in vivo to distinguish between these possibilities. Carotid arteries in inbred Lewis rats were excised 1 hour after balloon injury to give platelets the opportunity to adhere. These vessels were then transplanted from hypox into control animals and vice versa. At 48 hours, proliferation of smooth muscle cells in "control-to-hypox" transplants was 0.3% compared with 14.3% in "control-to-control" transplants, whereas vessels from hypox rats increased their indices to 4.8% if transplanted into control animals.(ABSTRACT TRUNCATED AT 250 WORDS)
Fingerle
J
J
Inst. Physiologie I, Chirurgische Klinik, University of Tübingen, FRG.
Faulmüller
A
A
Müller
G
G
Bowen-Pope
D F
DF
Clowes
M M
MM
Reidy
M A
MA
Clowes
A W
AW
D000818:Animals; D002339:Carotid Arteries / Q000637:transplantation; D002455:Cell Division; D002478:Cells, Cultured; D013006:Growth Hormone / Q000494:pharmacology; D007016:Hypophysectomy; D007334:Insulin-Like Growth Factor I / Q000502:physiology; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000166:cytology*; D010902:Pituitary Gland / Q000502:physiology*; D010982:Platelet-Derived Growth Factor / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley
D010982:Platelet-Derived Growth Factor; D007334:Insulin-Like Growth Factor I; D013006:Growth Hormone