[Detection of IgG receptor subtype on basophils using two-color FCM].
It was extremely difficult to phenotype basophils because they are most sparse among blood leukocytes. We developed a new method which identified basophils on the basis of negative reactivity with mixed monoclonal antibodies (CD2, CD14, CD16, CD19). Using this method we could detect Fc gamma R II (CDw32) but not Fc gamma R I (CD64) on basophil surface. The method seems useful for the study of clinical allergy and also of the biology of basophil.
Takahashi
K
K
Second Department of Internal Medicine, Okayama University Medical School.
Takada
M
M
D000911:Antibodies, Monoclonal; D001491:Basophils / Q000276:immunology* / Q000378:metabolism; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D006967:Hypersensitivity / Q000276:immunology; D017452:Receptors, IgG / Q000032:analysis*
D000911:Antibodies, Monoclonal; D017452:Receptors, IgG