Evaluation of a high-performance thin-layer chromatographic technique for the determination of salbutamol serum levels in clinical trials.
Salbutamol concentrations were determined by high-performance thin-layer chromatography in the sera of two sets of ten volunteers at hourly intervals for 6 h after taking one 8-mg slow-release tablet. The influence of time lapse in processing of serum samples, i.e. centrifugation, extraction and chromatography, was studied. A statistical significant instability of salbutamol in the sera of patients was found which was not present in standard drug-free serum samples spiked with salbutamol and used for construction of standard curves.
Le Roux
A M
AM
Department of Pharmacology, Faculty of Medicine, University of Stellenbosch, Tygerberg, South Africa.
Wium
C A
CA
Joubert
J R
JR
Van Jaarsveld
P P
PP
D000420:Albuterol / Q000097:blood*; D002855:Chromatography, Thin Layer / Q000379:methods*; D002986:Clinical Trials as Topic; D006801:Humans; D012016:Reference Values; D015203:Reproducibility of Results
D000420:Albuterol