Affinity of europium-labelled proteins A and G for immunoglobulin G from seven mammalian species.
Proteins A and G were each labelled with two different europium chelates (p-isothiocyanatophenyl-ethylenediaminetetraacetic acid and diethylenetriaminepentaacetic acid). Their affinities for IgG from rabbit, goat, horse, sheep, mouse, pig, and rat were then measured using time-resolved fluorescence. Protein G labelled with the second chelate was found to be especially effective in binding to goat and horse IgG.
Bacigalupo
M A
MA
Istituto Chimica Ormoni C.N.R., Milano, Italy.
Iacobello
C
C
Meroni
G
G
Ius
A
A
D000345:Affinity Labels; D000818:Animals; D001667:Binding, Competitive; D002614:Chelating Agents; D004492:Edetic Acid / Q000031:analogs & derivatives; D005063:Europium; D015200:Fluoroimmunoassay; D006041:Goats; D006736:Horses; D007074:Immunoglobulin G / Q000032:analysis* / Q000378:metabolism; D051379:Mice; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D004369:Pentetic Acid; D011817:Rabbits; D051381:Rats; D012756:Sheep; D013045:Species Specificity; D013205:Staphylococcal Protein A / Q000378:metabolism*; D013552:Swine
D000345:Affinity Labels; D002614:Chelating Agents; C029152:G-substrate; D007074:Immunoglobulin G; D009419:Nerve Tissue Proteins; D013205:Staphylococcal Protein A; D005063:Europium; D004369:Pentetic Acid; C043981:4-(2,3-bis(bis(carboxymethylamino))propyl)phenyl isothiocyanate; D004492:Edetic Acid