title: Structure of the proteolytic fragment F34 of calmodulin in the absence and presence of mastoparan as revealed by solution X-ray scattering.
abstract: The solution X-ray scattering technique has been applied to examine the conformations of the proteolytic fragment F34 (78Asp-148Lys) of calmodulin in the absence of both Ca2+ and mastoparan, in the presence of Ca2+ only, and in the presence of both Ca2+ and mastoparan. The radius of gyration and the molecular weight for the F34 fragment increased by 1.1 +/- 0.3 A and 19%, respectively, upon binding of both 2 mol of Ca2+/mol to the F34 fragment and mastoparan to form the tertiary complex. A smaller change was found for the Ca(2+)-saturated F34 fragment in the absence of mastoparan (0.3 +/- 0.3 A) without any change of the molecular weight. The analysis based on the small-angle scattering data showed that the F34 fragment in the presence of Ca2+ alone preserved the tertiary structure of the globular domain in the crystal to a great extent. Further analyses based on a two-domain model showed that the center-to-center distance between F34 and mastoparan is about 12.7 A, if the structure of the F34 fragment in the presence of mastoparan resembles that in the absence of mastoparan and if mastoparan in the complex retains an alpha-helical conformation. The modeling studies using their crystal structure coordinates have been made on the basis of the solution X-ray scattering data. The combined results support a model proposed by Persechini and Kretsinger [Persechini, A., & Kretsinger, R. H. (1988) J. Cardiovasc. Pharmacol. 12 (Suppl. 5), S1-S12], although the center-to-center distance between mastoparan and the F34 fragment is shorter by about 5 A than that in their model.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Macromolecular Research Laboratory, Faculty of Engineering, Yamagata University, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Izumi
authors.forename: M
authors.initials: M
authors.lastname: Wakita
authors.forename: H
authors.initials: H
authors.lastname: Yoshino
authors.forename: N
authors.initials: N
authors.lastname: Matsushima
mesh_terms: D000818:Animals; D001923:Brain Chemistry; D002147:Calmodulin / Q000737:chemistry*; D036341:Intercellular Signaling Peptides and Proteins; D010446:Peptide Fragments / Q000737:chemistry*; D010455:Peptides; D012542:Scattering, Radiation; D012996:Solutions; D013552:Swine; D014862:Wasp Venoms / Q000737:chemistry*; D014965:X-Rays
chemical_list: D002147:Calmodulin; D036341:Intercellular Signaling Peptides and Proteins; D010446:Peptide Fragments; D010455:Peptides; D012996:Solutions; D014862:Wasp Venoms; C037500:mastoparan