Analytical methods for identifying and quantitating deamidated sialic acid (2-keto-3-deoxy-D-glycero-D-galactonononic acid) and alpha 2----8-linked poly(oligo)nonulosonate residues in glycoconjugates.
In 1986 we reported the natural occurrence of deaminated neuraminic acid (2-keto-3-deoxy-D-glycero-D-galactonononic acid, KDN) in fish egg glycoprotein. Subsequently, we have shown that many types of sialic acid as well as KDN occur in polymeric chains, poly(oligo)-Sia and poly(oligo)KDN in nature. In this study we demonstrate that the conventional colorimetric and gas-liquid chromatographic methods used in the analysis of sialic acid can be applied to analysis of these new nonulosonate and poly(oligo)nonulosonates. We report that the thiobarbituric acid reaction can be used to analyze both free and bound KDN, but gives lower extinction values when applied to poly(oligo)KDN without prior hydrolysis. Further, the published hydrolytic and/or methanolytic procedures are suitable to release the terminal sialic acid residues, but are not appropriate for quantitative release of the nonulosonic acids from poly(oligo)nonulosonates. A new gas-liquid chromatographic procedure for the identification-quantitation of nonulosonates in poly(oligo)meric forms is described.
Kitajima
K
K
Department of Biophysics and Biochemistry, Faculty of Science, University of Tokyo, Japan.
Inoue
S
S
Kitazume
S
S
Inoue
Y
Y
D000818:Animals; D002240:Carbohydrate Sequence; D002849:Chromatography, Gas / Q000379:methods; D005260:Female; D006001:Glycoconjugates / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D008969:Molecular Sequence Data; D010053:Ovary / Q000737:chemistry; D011134:Polysaccharides / Q000032:analysis*; D013045:Species Specificity; D013400:Sugar Acids / Q000032:analysis*; D013696:Temperature; D013858:Thiobarbiturates / Q000032:analysis; D014337:Trout
D006001:Glycoconjugates; D011134:Polysaccharides; D013400:Sugar Acids; D013858:Thiobarbiturates; C077653:poly(oligo)(3-deoxyglycero-galacto-nonulosonate); C050008:3-deoxyglycero-galacto-nonulosonic acid; C029684:thiobarbituric acid