Quantification of neutral red pinocytosis by small numbers of adherent cells: comparative studies.
A colorimetric assay has been developed for studies on neutral red (NR) pinocytosis by small numbers (below 2 x 10(5)) of adherent cells cultured in 96 well plates. The NR uptake per cell mass was much higher in the sea urchin perivisceral adherent cells and human HL-60 cell line monolayers than in the murine and Atlantic salmon macrophages. The apparent difference points to the usefulness of this novel assay in comparative studies.
Płytycz
B
B
Laboratory of Evolutionary Immunology, Jagiellonian University, Kraków, Poland.
Rózanowska
M
M
Seljelid
R
R
D000818:Animals; D002448:Cell Adhesion; D002478:Cells, Cultured; D006801:Humans; D008264:Macrophages; D051379:Mice; D009499:Neutral Red; D010529:Peritoneal Cavity / Q000166:cytology; D010873:Pinocytosis; D012474:Salmon; D012617:Sea Urchins
D009499:Neutral Red