Schistosoma mansoni: adult males and females differentially express antigens encoded by repetitive genomic DNA.
Two repetitive DNA sequences have been characterized from Schistosoma mansoni which were transcribed into mRNAs and translated to give two families of cross-reactive proteins. One DNA element, which was present as a 230 bp Pst I fragment was arranged in tandem arrays of at least 17 copies in the genome. The second element, which could be localized to a 1800 bp Pst I fragment, was dispersed in the genome. The 1800 bp repeat was found on the mRNA encoding the 45 kDa polypeptide precursor of a potential surface antigen. This precursor was post-translationally modified to give a 50 kDa antigen (Sm50) which was expressed from the cercaria to the adult worm and in the egg. However, a proportion of this antigen was differentially modified in females and eggs to give a 60 kDa form. Two copies of the 230 bp repeat and one copy of the 1800 bp repeat were found on a second cDNA clone. The antiserum raised against the fusion protein of this clone recognized a family of cross-reactive proteins ranging from 14 to 70 kDa in size. The members of this family were also differentially expressed between the sexes. Consequently, two families of antigens have been identified which were both encoded by repetitive DNA elements and whose members were both differentially expressed in adult male and female worms.
Smith
A L
AL
Department of Pathology, University of Cambridge.
Huggins
M C
MC
Havercroft
J C
JC
D000818:Animals; D000947:Antigens, Helminth / Q000096:biosynthesis* / Q000235:genetics; D001345:Autoradiography; D015139:Blotting, Southern; D015153:Blotting, Western; D003429:Cross Reactions; D004247:DNA / Q000032:analysis / Q000235:genetics*; D005260:Female; D005786:Gene Expression Regulation; D008297:Male; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*; D015183:Restriction Mapping; D012550:Schistosoma mansoni / Q000235:genetics / Q000276:immunology*; D012737:Sex Factors; D014158:Transcription, Genetic
D000947:Antigens, Helminth; D012333:RNA, Messenger; D004247:DNA