Capsazepine inhibits low pH- and lactic acid-evoked release of calcitonin gene-related peptide from sensory nerves in guinea-pig heart.
In the isolated perfused guinea-pig heart low-pH buffer (pH 5), lactic acid (5 mM), capsaicin (10(-7) M) and nicotine (10(-4) M) all evoked a clear-cut release of calcitonin gene-related peptide (CGRP) from sensory nerves. Incubation with the proposed capsaicin receptor antagonist, capsazepine (10(-5) M), significantly reduced the CGRP release evoked not only by capsaicin but also by low pH and lactic acid, indicating a common mechanism of C-fibre activation, while the nicotine response remained unchanged.
Franco-Cereceda
A
A
Department of Pharmacology, Karolinska Institutet, Stockholm, Sweden.
Lundberg
J M
JM
D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000378:metabolism*; D002211:Capsaicin / Q000031:analogs & derivatives* / Q000494:pharmacology; D006168:Guinea Pigs; D006321:Heart / Q000294:innervation*; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007773:Lactates / Q000494:pharmacology*; D019344:Lactic Acid; D009412:Nerve Fibers / Q000187:drug effects* / Q000378:metabolism
D007773:Lactates; D019344:Lactic Acid; D015740:Calcitonin Gene-Related Peptide; C071423:capsazepine; D002211:Capsaicin