Significance of the positivity of some ELISA tests different as concerning the antigenic source in interpreting the western blot indeterminate sera in monitoring the epidemiology of high risk populations.
The paper analyses 50 pairs of sera pre-elevated within the "sentinel" epidemiological surveillance in a population exposed to a high risk at a 2 months interval. At the first determination all these sera showed an indeterminate Western Blot pattern; after 2 months, 93.7% revealed the occurrence of a certainly positive pattern. The paper is an analysis of the way in which different ELISA kits diagnosed at the first determination the Western Blot indeterminate sera. The purpose of the paper is to adopt a methodology of accurately diagnosing HIV infections in a population exposed to a high risk within an epidemiological surveillance of the "sentinel" type where the receiving of a second serum sample is quite difficult.
Neguţ
E
E
Cantacuzino Institute, Bucharest, Romania.
Mazilu
E
E
Băncescu
G
G
D015492:AIDS Serodiagnosis / Q000379:methods*; D000465:Algorithms; D015153:Blotting, Western; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005188:False Negative Reactions; D005189:False Positive Reactions; D015686:Gene Products, env / Q000276:immunology; D015483:HIV Antibodies / Q000097:blood; D015488:HIV Antigens / Q000276:immunology*; D016655:HIV Core Protein p24 / Q000276:immunology; D019302:HIV Envelope Protein gp160; D006679:HIV Seropositivity / Q000175:diagnosis*; D006801:Humans; D007223:Infant; D007326:Institutionalization; D011159:Population Surveillance; D011498:Protein Precursors / Q000276:immunology; D011933:Reagent Kits, Diagnostic; D012307:Risk Factors; D012680:Sensitivity and Specificity
D015686:Gene Products, env; D015483:HIV Antibodies; D015488:HIV Antigens; D016655:HIV Core Protein p24; D019302:HIV Envelope Protein gp160; D011498:Protein Precursors; D011933:Reagent Kits, Diagnostic