title: [Studies on the analytical method of a new anti-arrhythmic guan-fu base A in rabbit plasma].
abstract: Guan-fu base A, a new antiarrhythmic alkaloid with a structure of C20-diterpenoid, was first isolated from the tuber of Aconitum coreanum (Levl.) Raipaics in China in 1966. An analytical method of GC-ECD has been established in this paper for the determination of its concentration in rabbit plasma in order to study its pharmacokinetics in preclinical pharmacology. Guan-fu base A and the internal standard alprenolol (ALP) was found to react rapidly and quantitatively with trifluoroacetic anhydride (TFAA) to produce derivatives GFA-TFAA and ALP-TFAA which were identified by GC-MSD. An optimal GC-ECD system for the separation and determination of the derivatives following the sample preparation was established. There was a wide linear range of the method from 10-2 x 10(4) ng/ml (r = 0.9984). The average recovery of Guan-fu base A from the spiked plasma was 97.52%. The coefficient of variation of between-day and within-day were less than 9% and 7%, respectively. The method was used to determine Guan-fu base A in the plasma of 15 rabbits following intravenous administration of 3 dosages of 0.4, 2 and 10 mg per kg of body weight.
authors.affiliation: Department of Instrumental Analysis, Jinling Hospital, Nanjing.
authors.forename: L L
authors.initials: LL
authors.lastname: Yang
authors.forename: X J
authors.initials: XJ
authors.lastname: Yang
authors.forename: J H
authors.initials: JH
authors.lastname: Liu
authors.forename: N S
authors.initials: NS
authors.lastname: Li
authors.forename: N Y
authors.initials: NY
authors.lastname: Qiu
mesh_terms: D000470:Alkaloids / Q000097:blood*; D000818:Animals; D000889:Anti-Arrhythmia Agents / Q000097:blood*; D002849:Chromatography, Gas / Q000379:methods; D006576:Heterocyclic Compounds, 4 or More Rings; D011817:Rabbits
chemical_list: D000470:Alkaloids; D000889:Anti-Arrhythmia Agents; D006576:Heterocyclic Compounds, 4 or More Rings; C040615:Guan-fu base A