Transcriptional regulation of the redD transcriptional activator gene accounts for growth-phase-dependent production of the antibiotic undecylprodigiosin in Streptomyces coelicolor A3(2).
Transcription of redD, the activator gene required for production of the red-pigmented antibiotic undecylprodigiosin by Streptomyces coelicolor A3(2), showed a dramatic increase during the transition from exponential to stationary phase. The increase in redD expression was followed by transcription of redX, a biosynthetic structural gene, and the appearance of the antibiotic in the mycelium, and coincided with the intracellular appearance of ppGpp. However, ppGpp production elicited either by nutritional shift-down of, or addition of serine hydroxamate to, exponentially growing cultures had no stimulatory effect on redD transcription. The presence of redD on a multicopy plasmid resulted in elevated levels of the redD transcript and production of redX and undecylprodigiosin during exponential growth; the normal growth-phase-dependent production of undecylprodigiosin appeared to be mediated entirely through the redD promoter, which shows limited similarity to the consensus sequence for the major class of eubacterial promoters.
Takano
E
E
John Innes Institute, John Innes Centre, Norwich, UK.
Gramajo
H C
HC
Strauch
E
E
Andres
N
N
White
J
J
Bibb
M J
MJ
D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D001483:Base Sequence; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D005809:Genes, Regulator; D006159:Guanosine Tetraphosphate / Q000378:metabolism; D008969:Molecular Sequence Data; D011353:Prodigiosin / Q000031:analogs & derivatives* / Q000096:biosynthesis; D011401:Promoter Regions, Genetic; D013302:Streptomyces / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D015534:Trans-Activators / Q000235:genetics*; D014158:Transcription, Genetic
D001426:Bacterial Proteins; D015534:Trans-Activators; C077706:redD protein, Streptomyces coelicolor; D006159:Guanosine Tetraphosphate; C047347:undecylprodigiosin; D011353:Prodigiosin