Identification of human myocard proteins separated by two-dimensional electrophoresis.
N-Terminal sequencing, internal sequencing and amino acid analysis were used to identify twelve proteins of the human myocard two-dimensional gel electrophoresis (2-DE) pattern. Amino acid analysis was shown to be a powerful tool in addition to sequencing. The identification of a disease-associated N-terminally blocked protein by internal sequencing was not successful. The twelve identified proteins are the basis of a human myocard 2-DE database.
Jungblut
P
P
Deutsches Herzzentrum Berlin, Germany.
Otto
A
A
Regitz
V
V
Fleck
E
E
Wittmann-Liebold
B
B
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D016208:Databases, Factual; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D007526:Isoelectric Point; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009206:Myocardium / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification*
D000596:Amino Acids; D011506:Proteins