title: Histamine stimulates exocytosis in a sub-population of bovine adrenal chromaffin cells.
abstract: Both nicotinic stimulation and histamine are able to raise cytosolic free calcium concentration in the majority of cells in a population of bovine adrenal medullary chromaffin cells to comparable levels. Nevertheless, histamine induces much less catecholamine secretion than does nicotine. In order to test whether this is due to heterogeneity in the responses of chromaffin cells to histamine we examined exocytosis in response to nicotine and histamine using an immunofluorescence method based on staining with anti-DBH to detect inserted secretory vesicle membrane. The results show that while up to 98% of the chromaffin cells in culture undergo exocytosis in response to nicotine, histamine stimulates exocytosis in only a sub-population of cells.
authors.affiliation: Physiological Laboratory, University of Liverpool, UK.
authors.forename: N
authors.initials: N
authors.lastname: Pender
authors.forename: R D
authors.initials: RD
authors.lastname: Burgoyne
mesh_terms: D000313:Adrenal Medulla / Q000166:cytology / Q000187:drug effects*; D000818:Animals; D002395:Catecholamines / Q000378:metabolism; D002417:Cattle; D002838:Chromaffin System / Q000166:cytology / Q000187:drug effects*; D004299:Dopamine beta-Hydroxylase / Q000378:metabolism; D005089:Exocytosis / Q000187:drug effects*; D005455:Fluorescent Antibody Technique; D006632:Histamine / Q000494:pharmacology*; D009638:Norepinephrine / Q000378:metabolism; D013268:Stimulation, Chemical
chemical_list: D002395:Catecholamines; D006632:Histamine; D004299:Dopamine beta-Hydroxylase; D009638:Norepinephrine