title: The fate of the prosegment in the acute-phase and programmed synthesis of sapecin, an antibacterial peptide of the flesh fly (Sarcophaga peregrina).
abstract: The nucleotide sequence of sapecin cDNA suggested that this antibacterial peptide of the flesh fly (Sarcophaga peregrina) is produced from preprosapecin by post-translational processing. We examined the production of sapecin and its prosegment by radioimmunoassay under two different physiological conditions in which its gene is activated, assuming that the prosegment has some biological role. Results suggested that the prosegment is degraded selectively during production of sapecin. We also found that imaginal discs synthesize sapecin when cultured in the presence of 20-hydroxyecdysone.
authors.affiliation: Faculty of Pharmaceutical Sciences, University of Tokyo, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Homma
authors.forename: K
authors.initials: K
authors.lastname: Matsuyama
authors.forename: H
authors.initials: H
authors.lastname: Komano
authors.forename: S
authors.initials: S
authors.lastname: Natori
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D004175:Diptera / Q000254:growth & development / Q000378:metabolism*; D004441:Ecdysterone / Q000494:pharmacology; D005121:Extremities / Q000254:growth & development; D007301:Insect Hormones / Q000096:biosynthesis* / Q000737:chemistry; D019476:Insect Proteins; D007814:Larva / Q000378:metabolism; D008969:Molecular Sequence Data; D011498:Protein Precursors / Q000378:metabolism*; D011679:Pupa / Q000378:metabolism; D011863:Radioimmunoassay
chemical_list: D007301:Insect Hormones; D019476:Insect Proteins; D011498:Protein Precursors; C057638:sapecin protein, Sarcophaga; D004441:Ecdysterone