title: Spontaneous amplification of the ADH4 gene in Saccharomyces cerevisiae.
abstract: Five spontaneous amplifications of the ADH4 gene were identified among 1,894 antimycin A-resistant mutants isolated from a diploid strain after growth at 15 degrees. Four of these amplifications are approximately 40-kb linear extrachromosomal palindromes carrying telomere homologous sequences at each end similar to a previously isolated amplification. ADH4 is located at the extreme left end of chromosome VII, and the extrachromosomal fragments appear to be the fusion of two copies of the end of this chromosome. The fifth amplification is a chromosomal amplification carrying an extra copy of ADH4 on both homologs of chromosome VII. These results suggest that the ADH system can be used to study amplification in Saccharomyces cerevisiae.
authors.affiliation: Department of Biological Sciences, University of Cincinnati, Ohio 45221-0006.
authors.forename: M
authors.initials: M
authors.lastname: Dorsey
authors.forename: C
authors.initials: C
authors.lastname: Peterson
authors.forename: K
authors.initials: K
authors.lastname: Bray
authors.forename: C E
authors.initials: CE
authors.lastname: Paquin
mesh_terms: D000426:Alcohol Dehydrogenase / Q000235:genetics*; D004271:DNA, Fungal / Q000235:genetics; D005784:Gene Amplification; D015966:Gene Expression Regulation, Fungal; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics*
chemical_list: D004271:DNA, Fungal; D000426:Alcohol Dehydrogenase