title: Promoter probe and shuttle plasmids for Deinococcus radiodurans.
abstract: Two improved Deinococcus radiodurans-Escherichia coli shuttle vectors have been constructed. pI3 is a 16-kb plasmid that confers chloramphenicol resistance in D. radiodurans (CmR, cat) and ampicillin resistance in E. coli (ApR) and contains a multiple cloning site that does not interrupt sequences necessary for replication or drug resistance in either host. pI304 is a promoter-probe plasmid that is similar to pI3, but lacks the D. radiodurans promoting sequence for the cat gene, while retaining sequences necessary for replication.
authors.affiliation: Department of Pathology, F. E. Hébert School of Medicine, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814-4799.
authors.forename: C I
authors.initials: CI
authors.lastname: Masters
authors.forename: K W
authors.initials: KW
authors.lastname: Minton
mesh_terms: D000668:Ampicillin Resistance / Q000235:genetics; D001483:Base Sequence; D002702:Chloramphenicol Resistance / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D017353:Gene Deletion; D005822:Genetic Vectors; D006095:Gram-Positive Cocci / Q000235:genetics*; D015335:Molecular Probes; D008969:Molecular Sequence Data; D010957:Plasmids; D011401:Promoter Regions, Genetic
chemical_list: D004269:DNA, Bacterial; D015335:Molecular Probes