The regulation of prostaglandin biosynthesis in cells derived from human gestational tissues: effects of fetal calf serum.
We have evaluated the production of prostaglandin E2 (PGE2) and its regulation in amnion, chorion, and decidual cells in the presence and absence of fetal calf serum (FCS), and in the absence of FCS but with supplementation with substrate arachidonic acid (AA). Basal rates of PGE2 biosynthesis in amnion, chorion and decidual cell cultures were significantly reduced in the absence of FCS. The magnitudes of the responses to various stimulatory agents were different between cells from different tissues and the different culture media. We conclude that these different experimental conditions must be taken into account when interpreting the results of such in vitro experiments.
Edwin
S S
SS
Department of Obstetrics and Gynecology, University of Utah, School of Medicine, Salt Lake City 84132.
Mitchell
M D
MD
D000650:Amnion / Q000187:drug effects / Q000378:metabolism; D016718:Arachidonic Acid / Q000378:metabolism; D002823:Chorion / Q000187:drug effects / Q000378:metabolism; D003470:Culture Media; D003656:Decidua / Q000187:drug effects / Q000378:metabolism*; D015232:Dinoprostone / Q000096:biosynthesis*; D004815:Epidermal Growth Factor / Q000494:pharmacology; D005321:Extraembryonic Membranes / Q000187:drug effects / Q000378:metabolism*; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D007375:Interleukin-1 / Q000494:pharmacology; D015759:Ionomycin / Q000494:pharmacology; D011247:Pregnancy; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D003470:Culture Media; D007375:Interleukin-1; D016718:Arachidonic Acid; D015759:Ionomycin; D004815:Epidermal Growth Factor; D015232:Dinoprostone; D013755:Tetradecanoylphorbol Acetate