title: Diurnal variation and increase of disaccharidase activity in diabetic rats.
abstract: The small intestinal disaccharidase activity and its daily variation in the diabetic rat have not been well described. Therefore, the small intestinal disaccharidase (maltase, lactase and sucrase) activity and its daily profile were studied in streptozotocin-induced diabetic rats under physiological conditions. In diabetic rats, a similar pattern of diurnal variation of disaccharidase activity to control rats was observed, while the relationships between daily change of disaccharidase activity and that of food consumption suggested that there was a different mechanism of diurnal variation in diabetic rats. On the other hand, a significant increase of mean 24-h lactase and sucrase activities was noted in diabetic rats, while that of maltase was not significant. Using the in vitro incubation method, a significant correlation between glucose concentration and lactase or sucrase activity but not maltase activity was observed. However, insulin showed no effect on disaccharidase activity. Thus we clarified the presence of a diurnal variation of disaccharidase activity and an increase in its activity in diabetic rats. This change was suggested to be derived from high plasma glucose level.
authors.affiliation: Second Department of Internal Medicine, School of Medicine, University of the Ryukyus, Okinawa, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Nashiro
authors.forename: K
authors.initials: K
authors.lastname: Murakami
authors.forename: G
authors.initials: G
authors.lastname: Mimura
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D002940:Circadian Rhythm; D003921:Diabetes Mellitus, Experimental / Q000201:enzymology*; D004186:Disaccharidases / Q000032:analysis*; D005947:Glucose / Q000494:pharmacology; D007328:Insulin / Q000494:pharmacology; D007413:Intestinal Mucosa / Q000201:enzymology; D043322:Lactase; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013393:Sucrase / Q000032:analysis; D000520:alpha-Glucosidases / Q000032:analysis; D001616:beta-Galactosidase / Q000032:analysis
chemical_list: D001786:Blood Glucose; D007328:Insulin; D004186:Disaccharidases; D043322:Lactase; D000520:alpha-Glucosidases; D001616:beta-Galactosidase; D013393:Sucrase; D005947:Glucose