title: An 87-kilodalton glucan-binding protein of Streptococcus sobrinus B13.
abstract: An 87-kDa glucan-binding protein (GBP) of Streptococcus sobrinus B13 (serotype d) was isolated and purified from extracellular culture supernatant by using affinity chromatography on Sephadex G-50 and elution with a guanidine HCl gradient. Western blot (immunoblot) analysis showed it to be antigenically related, but not completely identical, to the 74-kDa GBP of Streptococcus mutans Ingbritt. The 87-kDa GBP has no glucosyltransferase activity. A possible role for this GBP in the cariogenicity of S. sobrinus B13 is suggested.
authors.affiliation: Dows Institute for Dental Research, University of Iowa College of Dentistry, Iowa City 52242.
authors.forename: C D
authors.initials: CD
authors.lastname: Wu-Yuan
authors.forename: R E
authors.initials: RE
authors.lastname: Gill
mesh_terms: D002352:Carrier Proteins / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification*; D005936:Glucans / Q000378:metabolism*; D005964:Glucosyltransferases / Q000032:analysis; D037102:Lectins; D016986:Streptococcus sobrinus / Q000737:chemistry*
chemical_list: D002352:Carrier Proteins; D005936:Glucans; D037102:Lectins; C037937:glucan-binding proteins; D005964:Glucosyltransferases