The adverse effects of l-methamphetamine on the development of explanted rat embryos.
The adverse effects of l-methamphetamine on the development of rat embryos were studied by means of a whole culture system. The embryos explanted at 12.5th days of gestational age were cultured for a total of 24 hours. After pre-culturing for 6 hours, 4 groups of embryos received additional culturing for 18 hours with saline or various concentrations of l-methamphetamine; 0.5, 2.0 and 5.0 mumol/dl. There was no significant difference in the survival rate among 4 groups. The groups over 2.0 mumol/dl of l-methamphetamine showed significant reduction of crown-rump length, somite number, protein content, DNA content, and protein/DNA ratio. It is suggested that over 2.0 mumol/dl of l-methamphetamine reduces the development but not the survival of rat embryos under whole culture system.
Ozaki
T
T
Hirosaki University Hospital, Department of Obstetrics and Gynecology, Hirosaki University School of Medicine, Japan.
Nakamura
Y
Y
Saito
Y
Y
D000818:Animals; D046508:Culture Techniques; D005314:Embryonic and Fetal Development / Q000187:drug effects*; D005260:Female; D008694:Methamphetamine / Q000009:adverse effects*; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley; D014022:Tissue Survival
D008694:Methamphetamine