Bivariate assay for cleaved atrial natriuretic factor (ANF(101-105/106-126)) in sheep plasma.
A bivariate assay has been developed to measure both ANF, and cleaved ANF (ANF(101-105/106-126)) the primary product of endopeptidase-24.11 action on rat ANF(101-126). The assay employed two different antisera directed to the carboxy-terminal and ring regions of ANF to enable discrimination and measurement of intact endogenous ANF(99-126) and infused cleaved ANF (rat ANF(101-105/106-126)) in sheep plasma extracts. The assay which was validated by HPLC analysis of plasma extracts, had sufficient accuracy and precision to measure the metabolic clearance rate and half-life of cleaved ANF when infused in normal sheep.
Yandle
T
T
Department of Endocrinology, Christchurch Hospital, New Zealand.
Sadler
B
B
Charles
C
C
Espiner
E
E
Richards
M
M
D000704:Analysis of Variance; D000818:Animals; D009320:Atrial Natriuretic Factor / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D006207:Half-Life; D010446:Peptide Fragments / Q000097:blood*; D011863:Radioimmunoassay; D012756:Sheep / Q000097:blood
D010446:Peptide Fragments; C077828:atrial natriuretic factor (101-105); C077829:atrial natriuretic factor (106-126); D009320:Atrial Natriuretic Factor; C043970:atrial natriuretic factor 26, rat