The FLP protein contacts both major and minor grooves of its recognition target sequence.
The FLP protein of the 2 microns plasmid of Saccharomyces cerevisiae promotes conservative site-specific recombination between DNA sequences that contain the FLP recognition target (FRT). FLP binds to each of the three 13 base pair symmetry elements in the FRT site in a site-specific manner. We have probed both major and minor groove contacts of FLP using dimethyl sulphate, monoacetyl-4-hydroxyaminoquinoline 1-oxide and potassium permanganate and find that the protein displays extensive interactions with residues of both the major and minor grooves of 10 base pairs of each symmetry element. We find no evidence that the FRT site assumes a single-stranded conformation upon FLP binding.
Panigrahi
G B
GB
Department of Molecular and Medical Genetics, University of Toronto, Ontario, Canada.
Beatty
L G
LG
Sadowski
P D
PD
D015108:4-Hydroxyaminoquinoline-1-oxide / Q000494:pharmacology; D001483:Base Sequence; D001665:Binding Sites; D004254:DNA Nucleotidyltransferases / Q000378:metabolism*; D004271:DNA, Fungal / Q000378:metabolism*; D004277:DNA, Single-Stranded / Q000378:metabolism; D005656:Fungal Proteins / Q000378:metabolism*; D006147:Guanine / Q000378:metabolism; D008745:Methylation; D008969:Molecular Sequence Data; D011196:Potassium Permanganate / Q000494:pharmacology; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D013379:Substrate Specificity; D013463:Sulfuric Acid Esters / Q000494:pharmacology; D013941:Thymine / Q000378:metabolism
D004271:DNA, Fungal; D004277:DNA, Single-Stranded; D005656:Fungal Proteins; D013463:Sulfuric Acid Esters; D011196:Potassium Permanganate; D015108:4-Hydroxyaminoquinoline-1-oxide; D006147:Guanine; D004254:DNA Nucleotidyltransferases; C045119:FLP recombinase; C007482:dimethyl sulfate; D013941:Thymine