title: An inducible expression system for the production of human lactoferrin in Aspergillus nidulans.
abstract: The production and secretion of human lactoferrin (hLF) in Aspergillus nidulans is described. The hLF cDNA was expressed under the control of the strong ethanol-inducible alcohol dehydrogenase (alcA) promoter. Recombinant hLF (re-hLF) is produced at levels up to 5 micrograms/ml. Approximately 30% of the re-hLF produced in this system is secreted into the growth medium. The re-hLF is indistinguishable from native hLF with respect to size and immunoreactivity. Furthermore, re-hLF is functional by the criterion of iron-binding capacity. The A. nidulans expression system offers an inexpensive, convenient method for the controlled production of mg amounts of biologically active mammalian glycoproteins.
authors.affiliation: Department of Cell Biology, Baylor College of Medicine, Houston, TX 77030.
authors.forename: P P
authors.initials: PP
authors.lastname: Ward
authors.forename: G S
authors.initials: GS
authors.lastname: May
authors.forename: D R
authors.initials: DR
authors.lastname: Headon
authors.forename: O M
authors.initials: OM
authors.lastname: Conneely
mesh_terms: D001233:Aspergillus nidulans; D015139:Blotting, Southern; D015153:Blotting, Western; D003001:Cloning, Molecular / Q000379:methods; D004247:DNA; D006801:Humans; D007501:Iron / Q000378:metabolism; D007781:Lactoferrin / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D010957:Plasmids; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism
chemical_list: D011994:Recombinant Proteins; D004247:DNA; D007501:Iron; D007781:Lactoferrin