title: Estrogen-induced morphological and immunohistochemical changes in stroma and epithelium of rat ventral prostate.
abstract: Prostatic smooth muscle cells have been regarded to play a major pathogenetic role during the development of benign prostatic hyperplasia (BPH) in elderly men. Altered hormonal signals (increased estrogen) have been made responsible for the "metabolic" transformation of prostatic smooth muscle cells, which were thought to produce increased amounts of connective tissue fibers observed in BPH. In order to find out the role of metabolically "activated" smooth muscle cells, hormone stimulation experiments were performed in male rats. The effects of androgen deprivation and estrogen stimulation were recorded by semiquantitative analysis of intermediate and myofilament proteins in stromal smooth muscle cells. In castrated or estrogen-treated or estrogen-treated and castrated animals, the reduction of the glandular lumen is the most obvious morphological alteration, accompanied by an increase in connective tissue. Regressive changes occurred most rapidly in castrated animals (already within the first week), slower in castrated estrogen-treated animals and still slower in normal estrogen-treated animals. Regression of the epithelium was accompanied by a marked decrease in immunoreactivity for prostatic binding protein (PBP) in castrated animals, while PBP immunoreactivity in estrogenized animals was retained for up to 6 weeks. Smooth muscle cells became atrophic in castrated animals. This effect was attenuated in estrogen-treated animals. There was no indication for enhanced collagen synthesis by smooth muscle cells. Actin and desmin-immunoreactivity were only slightly altered in experimental animals and showed a changed distribution pattern. Prostatic smooth muscle cells respond less markedly to hormonal alterations than do the fibroblasts.
authors.affiliation: Department of Anatomy and Cell Biology, Philipps University, Marburg, Federal Republic of Germany.
authors.forename: G Q
authors.initials: GQ
authors.lastname: Zhao
authors.forename: P M
authors.initials: PM
authors.lastname: Holterhus
authors.forename: I
authors.initials: I
authors.lastname: Dammshäuser
authors.forename: G
authors.initials: G
authors.lastname: Hoffbauer
authors.forename: G
authors.initials: G
authors.lastname: Aumüller
mesh_terms: D000818:Animals; D004847:Epithelial Cells; D004848:Epithelium / Q000187:drug effects / Q000378:metabolism; D004967:Estrogens / Q000494:pharmacology*; D007150:Immunohistochemistry; D008297:Male; D008855:Microscopy, Electron, Scanning; D009130:Muscle, Smooth / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D009919:Orchiectomy; D011467:Prostate / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
chemical_list: D004967:Estrogens