title: Metabolism of gallate in Penicillium simplicissimum.
abstract: The pathway for the degradation of gallate (3,4,5-trihydroxybenzoate) by a new strain of Penicillium was examined by in vivo and in vitro cell-free systems. The organism was identified as P. simplicissimum (Oud) Thom. It grew optimally at pH 5.5 and 27 degrees C, with 11.75 mM (0.2%) gallate added to the basal salt media. Mycelia grown on gallate accumulated protocatechuate in the medium, and had inducible activity for protocatechuate 3,4-dioxygenase as determined by spectrophotometric and oxygen electrode studies. A NADPH-dependent reductase in the cell-free extract reduced gallate to dihydrogallate. Maleylacetate reductase was also detected in the gallate induced cell-free extract, while beta-ketoadipate was detected as the product produced. The possible steps in the degradation of gallate are discussed.
authors.affiliation: Department of Biology and Biochemistry, Memorial University of Newfoundland, St. John's, Canada.
authors.forename: T R
authors.initials: TR
authors.lastname: Patel
authors.forename: N
authors.initials: N
authors.lastname: Hameed
authors.forename: S
authors.initials: S
authors.lastname: Armstrong
mesh_terms: D005707:Gallic Acid / Q000378:metabolism*; D010407:Penicillium / Q000378:metabolism*
chemical_list: D005707:Gallic Acid