Is a trypsin-like protease of mites a Der f III allergen?
A trypsin-like protease was purified from mite (Dermatophagoides farinae) fecal extract. In SDS-PAGE, the mite trypsin-like protease showed a single band at 34 kD. The purified trypsin-like protease possessed potent allergenic activity. Both the twenty N-terminal amino acid sequence and the amino acid composition of the purified protease were very similar to those of Der f III. These data strongly suggest that the trypsin-like protease in the mite is a Der f III allergen.
Ando
T
T
Section of Allergology, Research Laboratories, Torii & Co., Ltd.
Homma
R
R
Ino
Y
Y
Ito
G
G
Miyahara
A
A
Yamakawa
H
H
Iwaki
M
M
Okumura
Y
Y
Suko
M
M
Haida
M
M
D000485:Allergens / Q000276:immunology* / Q000302:isolation & purification; D000595:Amino Acid Sequence; D000818:Animals; D000941:Antigens / Q000276:immunology*; D039741:Antigens, Dermatophagoides; D008925:Mites / Q000201:enzymology* / Q000276:immunology; D008969:Molecular Sequence Data; D014357:Trypsin / Q000276:immunology* / Q000302:isolation & purification
D000485:Allergens; D000941:Antigens; D039741:Antigens, Dermatophagoides; D014357:Trypsin