Study of kidney and liver viability in the rat after exclusive aortic perfusion using intracellular ATP measurement.
To find whether the liver can be procured after exclusive aortic perfusion, three organ perfusion models were used in three groups of donor rats. Group 1 underwent liver wash-out via the portal vein; in group 2, the kidneys alone were perfused via the aorta; and group 3 underwent simultaneous aortic perfusion of liver and kidneys. All perfusion flow rates in the three groups were adjusted to physiological values. Harvested organs were transplanted and recipient animals were killed 4 h after transplantation to study liver and kidney viability by using intracellular ATP measurement. Liver ATP was lower (P < 0.005) in the portal perfusion group (group 1: 1.396 +/- 0.412) than in the aortic perfusion group (group 3: 2.181 +/- 0.061). Kidney ATP was comparable in groups 2 and 3:1.066 +/- 0.09 vs 1.059 +/- 0.273 (mumol/g) tissue). Liver cooling was quicker with portal perfusion than with the aortic flush (20 degrees C in 20 s vs 15 degrees C in 60 s). Aortic perfusion at a physiologic flow rate has no detrimental effect on renal viability studied by intracellular ATP measurement. We conclude that liver cooling via the aortic route only is a good alternative to portal perfusion and seems to give good preservation. Application of this observation to emergency procurement in humans is still the subject of controversy.
Bittard
H
H
Laboratory of Experimental Surgery, University of Paris, Bicêtre Hospital, France.
Chiche
L
L
Moukarzel
M
M
Douguet
D
D
Benoit
G
G
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001011:Aorta; D005069:Evaluation Studies as Topic; D006085:Graft Survival; D007668:Kidney / Q000378:metabolism; D016030:Kidney Transplantation; D008099:Liver / Q000378:metabolism; D016031:Liver Transplantation; D009926:Organ Preservation / Q000379:methods*; D010477:Perfusion; D051381:Rats; D011914:Rats, Inbred BN; D009927:Tissue and Organ Procurement
D000255:Adenosine Triphosphate