A new assay for lignin-type peroxidases employing the dye azure B.
The discovery in 1983 of fungal "ligninases" capable of catalyzing the peroxidation of nonphenolic aromatic lignin components has been seen as a major advance in understanding how certain basidiomycete fungi can completely degrade lignin. The ability of these lignin-type peroxidases to covert millimolar concentrations of veratryl alcohol to veratraldehyde, indicated by a change in the A310 of veratraldehyde, has become the standard assay for routine quantitation of LP activity. A new assay based on the oxidation of micromolar concentrations of the dye Azure B is presented. Although it is as simple and rapid as the veratryl alcohol assay, it appears to overcome some of the shortcomings of that assay. In particular, interference from UV- and short-wavelength visible-light-absorbing materials is greatly reduced and assay specificity is improved.
Archibald
F S
FS
Pulp and Paper Research Institute of Canada, Pointe Claire, Quebec.
D001399:Azure Stains; D001487:Basidiomycota / Q000378:metabolism; D001673:Biodegradation, Environmental; D007218:Industrial Microbiology / Q000379:methods*; D008031:Lignin / Q000378:metabolism; D010544:Peroxidases / Q000032:analysis* / Q000378:metabolism
D001399:Azure Stains; C025819:Azure B; D008031:Lignin; D010544:Peroxidases; C042858:lignin peroxidase