[Increasing the sensitivity of an immunohistochemical method for detecting carcinogen adducts with DNA].
Application of polyclonal antibodies against N7-methylguanine (N7-MG) for immunohistochemical detection of the DNA damage following exposure to methylnitrosourea in cultured cells (CHO and IAR-27) has been shown. The approaches were applied: a) an extraction of nuclei, and b) a silver intensification of the end product of peroxidase reaction. Both the approaches were effective in increasing the sensitivity of immunohistochemical detection of N7-MG, the latter technique being most sensitive. It is proposed that the silver intensification technique is very perspective to have a high levels of sensitivity and specificity, a sufficient contrast and preservation of the cell morphology. The efficiency of the new approach in application to the purposes of molecular epidemiology is discussed.
Mikheev
A M
AM
Asamoto
M
M
D000818:Animals; D016466:CHO Cells / Q000378:metabolism; D002273:Carcinogens / Q000493:pharmacokinetics*; D002478:Cells, Cultured / Q000378:metabolism; D006224:Cricetinae; D004247:DNA / Q000378:metabolism*; D004249:DNA Damage; D005347:Fibroblasts / Q000378:metabolism; D006147:Guanine / Q000031:analogs & derivatives / Q000378:metabolism; D007150:Immunohistochemistry / Q000379:methods; D051381:Rats; D012680:Sensitivity and Specificity; D016622:Silver Staining / Q000379:methods
D002273:Carcinogens; D006147:Guanine; C008450:7-methylguanine; D004247:DNA
Povyshenie chuvstvitel'nosti immunogistokhimicheskogo metoda vyiavleniia adduktov kantserogenov s DNK.