title: An FNR-dependent promoter from Escherichia coli is active and anaerobically inducible in Paracoccus denitrificans.
abstract: A transcriptional fusion of a synthetic FNR-dependent promoter derived from Escherichia coli has been introduced into Paracoccus denitrificans on a broad-host-range plasmid. The patterns of expression of beta-galactosidase from this fusion and from a control which is not regulated by FNR have been studied. The results indicate that P. denitrificans expresses a transcriptional regulator which has a very similar DNA-binding specificity to FNR, and responds to a similar physiological signal.
authors.affiliation: School of Biological Sciences, University of East Anglia, Norwich, UK.
authors.forename: S
authors.initials: S
authors.lastname: Spiro
mesh_terms: D000693:Anaerobiosis; D001426:Bacterial Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins; D015870:Gene Expression; D005798:Genes, Bacterial; D007506:Iron-Sulfur Proteins; D010231:Paracoccus denitrificans / Q000235:genetics* / Q000378:metabolism; D010957:Plasmids; D011401:Promoter Regions, Genetic; D014157:Transcription Factors / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; C060704:FNR protein, E coli; D007506:Iron-Sulfur Proteins; D014157:Transcription Factors