title: Flow cytometry for analyzing changes in irradiated and heated HeLa S3 cells.
abstract: In the present study, we have compared the distribution in various phases, DNA content, cell survival and ultrastructure of HeLa S3 cells after irradiation and/or hyperthermia. After 220 KV X-ray irradiation, the Do of the survival curve was 0.94 Gy, Dq was 1.3 Gy, and N was 4.26. After Ir-192 gamma-ray irradiation, the Do of the survival curve was 2.26 Gy, Dq was 3.9 Gy, and N was 5.7. The Do of the survival curve of HeLa S3 cells after treatment at 43.5 degrees C and 44 degrees C was 2.2 min and 1.6 min, respectively. Ultrastructural changes were also observed. The marked increase of the DNA content after 6 Gy irradiation corresponded with changes of distribution in various phases, which indicated a delay in the G2 + M phase. The survival fraction after 6 Gy irradiation was less than 1%. The changes of cell cycle distribution after Ir-192 irradiation were similar to those seen after X-ray exposure. The delay of G2 + M phase after hyperthermia was dose-dependent. An obvious delay of the G2 + M phases was also observed at 24 h after treatment with X-rays plus hyperthermia.
authors.affiliation: Cancer Institute, CAMS, Beijing.
authors.forename: S
authors.initials: S
authors.lastname: Wang
authors.forename: N
authors.initials: N
authors.lastname: Feng
authors.forename: J
authors.initials: J
authors.lastname: Li
authors.forename: Y
authors.initials: Y
authors.lastname: Yang
authors.forename: X
authors.initials: X
authors.lastname: Liu
mesh_terms: D002453:Cell Cycle; D002470:Cell Survival / Q000528:radiation effects*; D004273:DNA, Neoplasm / Q000378:metabolism; D005434:Flow Cytometry; D006367:HeLa Cells / Q000166:cytology / Q000528:radiation effects* / Q000648:ultrastructure; D006358:Hot Temperature; D006801:Humans; D007496:Iridium Radioisotopes; D014965:X-Rays
chemical_list: D004273:DNA, Neoplasm; D007496:Iridium Radioisotopes