title: Evaluation of a nisin-based germicidal formulation on teat skin of live cows.
abstract: A purified preparation of the nontoxic antimicrobial peptide, nisin (AMBICIN N), was used in the formulation of a germicidal sanitizer suitable for use on cow teats. The germicidal activity of the formulation against mastitis pathogens was measured on teat skin of live cows. The nisin-based formulation gave a mean log reduction of 3.90 against Staphylococcus aureus and 4.22 log reduction against Escherichia coli after exposure for 1 min to the germicide. This activity was comparable with that exhibited by a 1% iodophor teat dip but was significantly greater than that exhibited by the .1 and .5% iodophors and by the .5% chlorhexidine digluconate teat dips. The nisin-based formulation showed little or no potential for skin irritation after multiple application to skin, but iodophor and chlorhexidine digluconate teat dips showed significant potential for skin irritation in comparable studies.
authors.affiliation: Cornell University, Ithaca, NY 14850.
authors.forename: P M
authors.initials: PM
authors.lastname: Sears
authors.forename: B S
authors.initials: BS
authors.lastname: Smith
authors.forename: W K
authors.initials: WK
authors.lastname: Stewart
authors.forename: R N
authors.initials: RN
authors.lastname: Gonzalez
authors.forename: S D
authors.initials: SD
authors.lastname: Rubino
authors.forename: S A
authors.initials: SA
authors.lastname: Gusik
authors.forename: E S
authors.initials: ES
authors.lastname: Kulisek
authors.forename: S J
authors.initials: SJ
authors.lastname: Projan
authors.forename: P
authors.initials: P
authors.lastname: Blackburn
mesh_terms: D000818:Animals; D000891:Anti-Infective Agents, Local / Q000494:pharmacology* / Q000633:toxicity; D002417:Cattle / Q000382:microbiology*; D002710:Chlorhexidine / Q000031:analogs & derivatives / Q000494:pharmacology; D005069:Evaluation Studies as Topic; D005260:Female; D007466:Iodophors / Q000494:pharmacology; D007509:Irritants / Q000633:toxicity; D008321:Mammary Glands, Animal / Q000187:drug effects* / Q000382:microbiology; D008826:Microbial Sensitivity Tests; D009561:Nisin / Q000494:pharmacology* / Q000633:toxicity; D012867:Skin / Q000187:drug effects* / Q000382:microbiology
chemical_list: D000891:Anti-Infective Agents, Local; D007466:Iodophors; D007509:Irritants; D009561:Nisin; C010882:chlorhexidine gluconate; D002710:Chlorhexidine