title: Interlaboratory study of the IFCC method for alanine aminotransferase performed with use of a partly purified reference material.
abstract: We present the results of a study on performance of a reference material for alanine aminotransferase (ALT, EC 2.6.1.2) and the corresponding IFCC-approved method in an interlaboratory trial involving 13 laboratories. The ALT material was partly purified from pig heart (specific activity, 150 kU/g) and was essentially free of six potentially contaminating enzyme activities, including aspartate aminotransferase (EC 2.6.1.1). The partly purified ALT was lyophilized in a triethanolamine-buffered matrix, pH 6.4, containing bovine serum albumin and saccharose. Under these conditions, the predicted yearly loss of activity was 0.02% at 4 degrees C and < 0.01% at -20 degrees C. The final blank-corrected results of the accepted set of data gave a mean (SD) of 128.5 (5.1) U/L. The among-laboratory SD was 4.6 U/L and the within-laboratory SD was 2.0 U/L. The certified ALT catalytic concentration in the reconstituted material was 129 U/L with a 0.95 confidence interval of +/- 4 U/L.
authors.affiliation: Laboratoire du Centre de Médecine Préventive et Centre du Médicament, UA CNRS No 597, Nancy, France.
authors.forename: F
authors.initials: F
authors.lastname: Schiele
authors.forename: J
authors.initials: J
authors.lastname: Muller
authors.forename: E
authors.initials: E
authors.lastname: Colinet
authors.forename: G
authors.initials: G
authors.lastname: Siest
authors.forename: P
authors.initials: P
authors.lastname: Arzoglou
authors.forename: H
authors.initials: H
authors.lastname: Brettschneider
authors.forename: D H
authors.initials: DH
authors.lastname: Calam
authors.forename: F
authors.initials: F
authors.lastname: Ceriotti
authors.forename: G
authors.initials: G
authors.lastname: Férard
authors.forename: J
authors.initials: J
authors.lastname: Frei
mesh_terms: D000410:Alanine Transaminase / Q000097:blood* / Q000378:metabolism / Q000592:standards*; D000818:Animals; D002384:Catalysis; D004340:Drug Contamination; D004795:Enzyme Stability; D005612:Freeze Drying; D006801:Humans; D007753:Laboratories / Q000592:standards*; D009206:Myocardium / Q000201:enzymology; D011786:Quality Control; D011933:Reagent Kits, Diagnostic / Q000592:standards; D012015:Reference Standards; D013053:Spectrophotometry / Q000592:standards; D013379:Substrate Specificity; D013552:Swine
chemical_list: D011933:Reagent Kits, Diagnostic; D000410:Alanine Transaminase