Scattering of the rRNA genes on the physical map of the circular chromosome of Leptospira interrogans serovar icterohaemorrhagiae.
Leptospira interrogans is a pathogenic bacterium with a low G+C content (34 to 39%). The restriction enzymes NotI, AscI, and SrfI cut the chromosome of L. interrogans serovar icterohaemorrhagiae into 13, 3, and 5 fragments separable by one- and two-dimensional pulsed-field gel electrophoresis (PFGE). The genome is composed of a circular 4.6-Mbp chromosome and a 0.35-Mbp extrachromosomal element. A physical map of the chromosome was constructed for NotI, AscI, and SrfI by using single and double digests, or partial NotI digests obtained at random or by cross-protection of NotI sites by FnuDII methylase, and linking clones. rRNA genes were found to be widely scattered on the chromosome.
Baril
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Unité de Bactériologie Moléculaire et Médicale, Institut Pasteur, Paris, France.
Herrmann
J L
JL
Richaud
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C
Margarita
D
D
Girons
I S
IS
D001483:Base Sequence; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D003001:Cloning, Molecular; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D005798:Genes, Bacterial / Q000235:genetics*; D016680:Genome, Bacterial; D007921:Leptospira interrogans / Q000235:genetics*; D008969:Molecular Sequence Data; D012335:RNA, Ribosomal / Q000235:genetics*; D012703:Serotyping
D012335:RNA, Ribosomal; C078382:endodeoxyribonuclease AscI; C078383:endodeoxyribonuclease SrfI; D015252:Deoxyribonucleases, Type II Site-Specific; C038565:GCGGCCGC-specific type II deoxyribonucleases