Local radiolabeling of the 68 kDa neurofilament protein in rat sciatic nerves.
Rat sciatic nerve segments, 4.5 cm distal to the dorsal root ganglion (DRG), were incubated in vivo with [35S]methionine. Fluorography of 2-D polyacrylamide gels of the nerve proteins demonstrated the labeling of the 68-kDa neurofilament protein, which was identified by immunoblotting. This experimental design excludes the dorsal root ganglion as the source of the radiolabeled neurofilament protein and suggests that this neuron-specific protein may be synthesized in axons.
Sotelo
J R
JR
División Biofisica, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay.
Benech
C R
CR
Kun
A
A
D000818:Animals; D015151:Immunoblotting; D066298:In Vitro Techniques; D007553:Isotope Labeling; D008297:Male; D008715:Methionine / Q000378:metabolism; D009186:Myelin Sheath / Q000502:physiology; D009409:Nerve Crush; D016900:Neurofilament Proteins / Q000032:analysis / Q000096:biosynthesis*; D051381:Rats; D012584:Sciatic Nerve / Q000378:metabolism*; D013050:Spectrometry, Fluorescence
D016900:Neurofilament Proteins; D008715:Methionine