Carbamylation and glycosylation of haemoglobin in vitro: effects of cyanate and glucose.
An in vitro system that is independent of any haemodynamic influence and other physiological controls was designed to study the formation of carbamylated and glycosylated haemoglobin, by the use of cyanate and glucose respectively, incubated with whole-blood from normal healthy subjects. In this system the formation of glycosylated haemoglobin is more favourable than that of carbamylated haemoglobin. The effect of glucose in reducing the formation of carbamylated haemoglobin is greater than the effect of cyanate on the formation of glycoslyated haemoglobin. There is a strong correlation coefficient (r = 0.9, P < 0.005) for the relationship between the formation of carbamylated and glycosylated haemoglobin in this system.
Ationu
A
A
Child Health Department, St. George's Hospital Medical School, London, England, UK.
D003485:Cyanates; D005947:Glucose; D006442:Glycated Hemoglobin / Q000138:chemical synthesis*; D006441:Hemoglobin A / Q000031:analogs & derivatives* / Q000138:chemical synthesis; D006454:Hemoglobins; D006801:Humans; D066298:In Vitro Techniques
D003485:Cyanates; D005947:Glucose; D006442:Glycated Hemoglobin; D006441:Hemoglobin A; D006454:Hemoglobins; C022017:hemoglobin A, carbamylated