Efficient solution-phase synthesis of multiple O-phosphoseryl-containing peptides related to casein and statherin.
The multiple Ser(P)-containing peptides, H-Ser(P)-Ser(P)-Ser(P)-Glu-Glu-NHMe.TFA, H-Asp-Ser(P)-Ser(P)-Glu-Glu-NHMe.TFA and H-Glu-Ser(P)-Ser(P)-Glu-Glu-NHMe.TFA were prepared by the use of Boc-Ser(PO3Ph2)-OH in the Boc mode of solution phase peptide synthesis followed by platinum-mediated hydrogenolytic de-protection of the Ser(PO3Ph2)-containing peptides. The protected peptides were assembled using the mixed anhydride coupling methods with 40% TFA/CH2Cl2 used for removal of the Boc group from intermediate Boc-protected peptides.
Perich
J W
JW
Biochemistry and Molecular Biology Unit, School of Dental Science, University of Melbourne, Victoria, Australia.
Kelly
D P
DP
Reynolds
E C
EC
D000595:Amino Acid Sequence; D002364:Caseins / Q000138:chemical synthesis*; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis*; D010750:Phosphoproteins / Q000138:chemical synthesis*; D010768:Phosphoserine / Q000138:chemical synthesis*; D012471:Salivary Proteins and Peptides / Q000138:chemical synthesis*
D002364:Caseins; D010455:Peptides; D010750:Phosphoproteins; C018149:STATH protein, human; D012471:Salivary Proteins and Peptides; D010768:Phosphoserine