title: Arsenic analysis II: rapid separation and quantification of inorganic arsenic plus metabolites and arsenobetaine from urine.
abstract: We describe the rapid separation of inorganic arsenic plus metabolites from arsenobetaine or seafood arsenic in urine. Traditional, high-pressure liquid chromatography is replaced by disposable silica-based cation-exchange cartridges for this separation. Both fractions are quickly separated and collected for analysis by atomic absorption spectrophotometry. Analytical recovery of both fractions is > or = 95%, with an overall precision (CV) ranging from 1.6% to 6.4%. Using this method, we correctly identified the sources of arsenic exposure, whether of inorganic or seafood origin, in 11 urine specimens supplied by the Centre de Toxicologie du Quebec.
authors.affiliation: Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN 55905.
authors.forename: D E
authors.initials: DE
authors.lastname: Nixon
authors.forename: T P
authors.initials: TP
authors.lastname: Moyer
mesh_terms: D000818:Animals; D001151:Arsenic / Q000652:urine*; D001152:Arsenicals / Q000652:urine*; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000706:statistics & numerical data; D004284:Dogfish; D006801:Humans; D009132:Muscles / Q000737:chemistry; D011786:Quality Control; D013054:Spectrophotometry, Atomic
chemical_list: D001152:Arsenicals; D001151:Arsenic; C038992:arsenobetaine