Interferon-mediated intracellular signalling. Modulation of different phospholipase activities in Burkitt lymphoma cells.
The effect of interferon-alpha on Daudi lymphoma cells either sensitive or resistant to the action of this cytokine has been analysed in terms of phospholipase C (PLC) and D (PLD) activities. Results have shown a combined modulation of PIP2-specific phospholipase C and phospholipase D. In particular, a decreased activity of PIP2-specific PLC has been found, concomitant to a PLD-mediated phosphatidylcholine hydrolysis, suggesting that the intracellular signalling activated by interferon in Daudi cells involves a phospholipase D/phosphohydrolase pathway.
Cataldi
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Istituto di Morfologia Umana Normale, Università di Chieti, Italy.
Rana
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di Primio
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Bareggi
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Lisio
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Toto
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Miscia
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S
D002051:Burkitt Lymphoma / Q000201:enzymology; D004075:Diglycerides / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D000077190:Interferon alpha-2; D016898:Interferon-alpha / Q000494:pharmacology*; D010712:Phosphatidic Acids / Q000378:metabolism; D010739:Phospholipase D / Q000378:metabolism*; D011433:Propranolol / Q000494:pharmacology; D011994:Recombinant Proteins; D015398:Signal Transduction; D014407:Tumor Cells, Cultured; D010738:Type C Phospholipases / Q000378:metabolism*
D004075:Diglycerides; D000077190:Interferon alpha-2; D016898:Interferon-alpha; D010712:Phosphatidic Acids; D011994:Recombinant Proteins; D011433:Propranolol; D010738:Type C Phospholipases; D010739:Phospholipase D