title: Molecular characterization of a pea beta-1,3-glucanase induced by Fusarium solani and chitosan challenge.
abstract: beta-glucanases are prominent proteins in pea endocarp tissue responding to fungal infection. We have cloned and sequenced a partial pea cDNA clone, pPIG312, corresponding to a beta-1,3-glucanase in pea pods challenged with the incompatible pathogen Fusarium solani f. sp. phaseoli. The insert from the partial pea cDNA was used to probe a genomic library derived from pea leaves of the same cultivar. One of the genomic clones, pPIG4-3, contained the complete coding sequence for a mature beta-1,3-glucanase protein. The predicted amino acid sequence of the pea beta-1,3-glucanase has 78% identity to bean beta-1,3-glucanase, 62% and 60% to two tobacco beta-1,3-glucanases, 57% to soybean beta-1,3-glucanase, 51% to barley beta-1,3-glucanase, and 48% to barley beta-1,3-1,4-glucanase. Genomic Southern analysis indicates that the pea genome contains only one beta-1,3-glucanase gene corresponding to the probe used in this study. Accumulation of beta-1,3-glucanase mRNA homologous with the pPIG312 probe was detected in pea pods within 4 to 8 h after challenge with F. solani f. sp. phaseoli, f. sp. pisi, a compatible strain, or the elicitor, chitosan. In the incompatible reaction, mRNA accumulation remained high for 48h, whereas it rapidly decreased in the compatible reaction. After fungal inoculation of whole pea seedlings, the enhanced mRNA accumulation occurred mainly in the basal region (lower stem and root). This beta-1,3-glucanase mRNA was constitutively expressed in the roots of pea seedlings.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Plant Pathology, Washington State University, Pullman 99164-6430.
authors.forename: M M
authors.initials: MM
authors.lastname: Chang
authors.forename: L A
authors.initials: LA
authors.lastname: Hadwiger
authors.forename: D
authors.initials: D
authors.lastname: Horovitz
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D002686:Chitin / Q000031:analogs & derivatives* / Q000494:pharmacology; D048271:Chitosan; D003001:Cloning, Molecular; D004247:DNA; D015342:DNA Probes; D007887:Fabaceae / Q000201:enzymology* / Q000235:genetics / Q000382:microbiology; D005670:Fusarium / Q000235:genetics / Q000502:physiology*; D015971:Gene Expression Regulation, Enzymologic; D043326:Glucan 1,3-beta-Glucosidase; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics*; D010946:Plants, Medicinal; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D001617:beta-Glucosidase / Q000235:genetics*
chemical_list: D015342:DNA Probes; D010940:Plant Proteins; D002686:Chitin; D004247:DNA; D048271:Chitosan; D001617:beta-Glucosidase; D043326:Glucan 1,3-beta-Glucosidase