Evidence for sex steroid receptors in feline brainstem.
Specific binding sites for estrogens, androgens, and progestins were found in cytosol from perfused cat brainstem with the use of a steroid binding assay. These sites resemble 'classical' steroid receptors in ligand specificity as well as in sedimentation properties (sedimentation constant 8-10S on sucrose density gradient). The presence of estrogen receptor in brainstem extracts was also confirmed by an enzyme immunoassay employing monoclonal antibodies directed against human estrogen receptor. This finding may be relevant to known influences of sex steroids on respiration.
Chrapusta
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Endocrinology Laboratory, Maria Sklodowska-Curie Memorial Cancer Center and Institute of Oncology, Warsaw, Poland.
Grieb
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D000818:Animals; D001933:Brain Stem / Q000378:metabolism*; D002415:Cats; D002467:Cell Nucleus / Q000378:metabolism; D002499:Centrifugation, Density Gradient; D003600:Cytosol / Q000378:metabolism; D005260:Female; D012739:Gonadal Steroid Hormones; D066298:In Vitro Techniques; D008297:Male; D011944:Receptors, Androgen / Q000378:metabolism; D011960:Receptors, Estrogen / Q000378:metabolism; D011980:Receptors, Progesterone / Q000378:metabolism; D011987:Receptors, Steroid / Q000187:drug effects*
D012739:Gonadal Steroid Hormones; D011944:Receptors, Androgen; D011960:Receptors, Estrogen; D011980:Receptors, Progesterone; D011987:Receptors, Steroid