Nuclear proteins of hamster hepatoma after administration of antitumour agents.
One- and two-dimensional gel electrophoretic analysis of nuclear proteins of Kirkman-Robbins hepatoma was used to study the effects of the tumour growth inhibitors methotrexate (MTX) and acyclonucleoside (DHPQtB) on protein composition. MTX and DHPQtB inhibited Kirkman-Robbins hepatoma growth by 89.2 +/- 3.5% and 16.3 +/- 6.1% respectively. The biosynthesis and/or metabolism of some polypeptide spots was affected by these antitumour agents, especially among components with molecular wt/isoelectric points of 52,000-64,000/4.9-5.5, 69,000-78,000/5.0-5.9 and 88,000-100,000/5.1-5.9.
Kiliańska
Z
Z
Department of Cytobiochemistry, University of Lódź, Poland.
Lipińska
A
A
Krajewska
W M
WM
Gaczyński
M
M
Bartkowiak
J
J
Kłyszejko-Stefanowicz
L
L
D000818:Animals; D000964:Antimetabolites, Antineoplastic / Q000494:pharmacology* / Q000627:therapeutic use; D006224:Cricetinae; D003864:Depression, Chemical; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D008114:Liver Neoplasms, Experimental / Q000737:chemistry* / Q000188:drug therapy; D008297:Male; D008647:Mesocricetus; D008727:Methotrexate / Q000494:pharmacology* / Q000627:therapeutic use; D008970:Molecular Weight; D009363:Neoplasm Proteins / Q000302:isolation & purification / Q000378:metabolism*; D009376:Neoplasms, Hormone-Dependent / Q000737:chemistry / Q000188:drug therapy; D009687:Nuclear Proteins / Q000302:isolation & purification / Q000378:metabolism*; D011799:Quinazolines / Q000494:pharmacology* / Q000627:therapeutic use; D013739:Testosterone
D000964:Antimetabolites, Antineoplastic; D009363:Neoplasm Proteins; D009687:Nuclear Proteins; D011799:Quinazolines; C078529:1-N-(1,3-dihydroxy-2-propoxymethyl)-6-(tert-butyl)-5,6,7,8-tetrahydro-2,4-quinazolinedione; D013739:Testosterone; D008727:Methotrexate