Transfection of E. coli with lambda DNA by electroporation.
In the ambit of the B. subtilis genoma sequencing and mapping project, we have set up an electroporation method to transfect E. coli cells with lambda DNA. This methodology presents features that make it preferable to traditional in vitro packaging for some purposes. Here we will illustrate the experimental procedure and the possible applications.
Magistrelli
C
C
Institute of Medical Microbiology, University of Milan, Italy.
Colombo
E
E
Tognoni
A
A
Grandi
G
G
D010582:Bacteriophage lambda / Q000235:genetics*; D004279:DNA, Viral / Q000235:genetics*; D004560:Electricity; D004926:Escherichia coli / Q000235:genetics*; D014162:Transfection / Q000379:methods*
D004279:DNA, Viral