title: Is a trypsin-like protease of mites a Der f III allergen?
abstract: A trypsin-like protease was purified from mite (Dermatophagoides farinae) fecal extract. In SDS-PAGE, the mite trypsin-like protease showed a single band at 34 kD. The purified trypsin-like protease possessed potent allergenic activity. Both the twenty N-terminal amino acid sequence and the amino acid composition of the purified protease were very similar to those of Der f III. These data strongly suggest that the trypsin-like protease in the mite is a Der f III allergen.
authors.affiliation: Section of Allergology, Research Laboratories, Torii & Co., Ltd.
authors.forename: T
authors.initials: T
authors.lastname: Ando
authors.forename: R
authors.initials: R
authors.lastname: Homma
authors.forename: Y
authors.initials: Y
authors.lastname: Ino
authors.forename: G
authors.initials: G
authors.lastname: Ito
authors.forename: A
authors.initials: A
authors.lastname: Miyahara
authors.forename: H
authors.initials: H
authors.lastname: Yamakawa
authors.forename: M
authors.initials: M
authors.lastname: Iwaki
authors.forename: Y
authors.initials: Y
authors.lastname: Okumura
authors.forename: M
authors.initials: M
authors.lastname: Suko
authors.forename: M
authors.initials: M
authors.lastname: Haida
mesh_terms: D000485:Allergens / Q000276:immunology* / Q000302:isolation & purification; D000595:Amino Acid Sequence; D000818:Animals; D000941:Antigens / Q000276:immunology*; D039741:Antigens, Dermatophagoides; D008925:Mites / Q000201:enzymology* / Q000276:immunology; D008969:Molecular Sequence Data; D014357:Trypsin / Q000276:immunology* / Q000302:isolation & purification
chemical_list: D000485:Allergens; D000941:Antigens; D039741:Antigens, Dermatophagoides; D014357:Trypsin