Processing and localization of Dengue virus type 2 polyprotein precursor NS3-NS4A-NS4B-NS5.
Processing of dengue virus type 2 polyprotein precursor NS3-NS4A-NS4B-NS5 could be mediated by the catalytically active NS3 protease domain and NS2B in trans at the dibasic sites NS3-NS4A and NS4B-NS5. Subcellular localization of the unprocessed precursor NS3-NS4A-NS4B-NS5 showed that it was confined to a distinct subcellular organelle in the cytoplasm, which was distinct from the distribution of the mature NS5.
Zhang
L
L
Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, Kansas City 66103.
Mohan
P M
PM
Padmanabhan
R
R
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D003716:Dengue Virus / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D015388:Organelles / Q000378:metabolism / Q000648:ultrastructure; D010957:Plasmids; D016133:Polymerase Chain Reaction / Q000379:methods; D011498:Protein Precursors / Q000032:analysis / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D017361:Viral Nonstructural Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*
D009838:Oligodeoxyribonucleotides; D011498:Protein Precursors; D017361:Viral Nonstructural Proteins