Reversible immobilization of antibodies on magnetic beads.
A streptavidin-biotin system was utilized to prepare an antibody-polyadenylic acid conjugate which was subsequently attached to commercially available magnetic beads, Dynabeads oligo(dT)25. Biotinylated polyadenylic acid was combined with streptavidin and the resulting polyadenylic acid-streptavidin was conjugated with an antibody-biotin derivative. The immobilized antibody-polyadenylic acid conjugate was separated from the reaction mixture by hybridization with complementary oligonucleotide immobilized on the surface of Dynabeads oligo(dT)25. The immobilized antibody-polyadenylic acid can be released from the carrier, utilizing low-ionic-strength buffers. The system is intended to be utilized in cell sorting, using immobilized antibodies against cell surface antigens. Dissociation of antibody-containing conjugate from magnetic beads is essential for the isolation of viable cells via positive cell sorting.
Scouten
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WH
Chemistry Department, Baylor University, Waco, Texas 76798.
Konecny
P
P
D000249:Adenosine Monophosphate / Q000737:chemistry; D000906:Antibodies / Q000737:chemistry*; D001426:Bacterial Proteins / Q000737:chemistry*; D001710:Biotin / Q000737:chemistry*; D002469:Cell Separation / Q000379:methods; D008280:Magnetics; D019809:Streptavidin
D000906:Antibodies; D001426:Bacterial Proteins; D000249:Adenosine Monophosphate; D001710:Biotin; D019809:Streptavidin