Structure of a C-type mannose-binding protein complexed with an oligosaccharide.
C-type (Ca(2+)-dependent) animal lectins such as mannose-binding proteins mediate many cell-surface carbohydrate-recognition events. The crystal structure at 1.7 A resolution of the carbohydrate-recognition domain of rat mannose-binding protein complexed with an oligomannose asparaginyl-oligosaccharide reveals that Ca2+ forms coordination bonds with the carbohydrate ligand. Carbohydrate specificity is determined by a network of coordination and hydrogen bonds that stabilizes the ternary complex of protein, Ca2+ and sugar. Two branches of the oligosaccharide crosslink neighbouring carbohydrate-recognition domains in the crystal, enabling multivalent binding to a single oligosaccharide chain to be visualized directly.
Weis
W I
WI
Department of Biochemistry and Molecular Biophysics, Columbia University, New York, New York 10032.
Drickamer
K
K
Hendrickson
W A
WA
D000117:Acetylglucosamine / Q000378:metabolism; D000818:Animals; D001216:Asparagine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D002118:Calcium / Q000378:metabolism*; D002352:Carrier Proteins / Q000737:chemistry* / Q000378:metabolism*; D003461:Crystallography; D005643:Fucose / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D008358:Mannose / Q000378:metabolism; D037601:Mannose-Binding Lectin; D008958:Models, Molecular; D008968:Molecular Conformation; D015394:Molecular Structure; D009844:Oligosaccharides / Q000737:chemistry* / Q000378:metabolism*; D051381:Rats; D013379:Substrate Specificity
D002352:Carrier Proteins; D037601:Mannose-Binding Lectin; D009844:Oligosaccharides; C069858:mannose binding protein A; D005643:Fucose; C072827:(mannose)6-(N-acetylglucosamine)2-asparagine; D001216:Asparagine; D005947:Glucose; D008358:Mannose; D002118:Calcium; D000117:Acetylglucosamine