Quantitation of urinary hydroxypyridinium cross-links from collagen by high-performance liquid chromatography.
Pyridinoline and deoxypyridinoline are intermolecular cross-links in mature collagen in bone and cartilage. The urinary excretion of the two compounds correlates well to bone turnover. A fast, sensitive, and accurate isocratic ion-pairing reverse-phase high-performance liquid chromatography method for measurement of pyridinoline and deoxypyridinoline in urine has been established. Intra- and inter-assay precision were 5-7% and 12-14%, respectively. Recovery for pyridinoline was 97.4% and for deoxypyridinoline 94.3%. The detection limit was 0.4 pmol. Pyridinoline:creatinine and deoxypyridinoline: creatinine ratios in healthy subjects, were 38.8 nmol:mmol and 13.0 nmol:mmol, respectively. Increased values of both cross-links were observed in children, in the age group 20-29 in both sexes, and in post-menopausal women.
Kollerup
G
G
Department of Medicine, Sundby Hospital, Copenhagen, Denmark.
Thamsborg
G
G
Bhatia
H
H
Sørensen
O H
OH
D000375:Aging / Q000502:physiology; D000596:Amino Acids / Q000652:urine*; D001842:Bone and Bones / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D003094:Collagen / Q000378:metabolism*; D003404:Creatinine / Q000652:urine; D005260:Female; D006801:Humans; D008297:Male; D008593:Menopause / Q000502:physiology; D012016:Reference Values; D012727:Sex Characteristics
D000596:Amino Acids; C015484:pyridinoline; C036020:deoxypyridinoline; D003094:Collagen; D003404:Creatinine