Cell adhesion molecule L1 guides cell migration in primary reaggregation cultures of mouse cerebellar cells.
We demonstrate a new assay in vitro using reaggregated cerebellar neurons in order to test the role of the L1 molecule in migration events. Cells other than neurons were eliminated in these cultures. Cerebellar neuron reaggregates plated on L1 molecule substrates migrated at rates similar to those observed in vivo. This migration was blocked by anti-L1 antibody. These findings suggest that this culture could provide a useful system with which to directly study relationships between neuron migration and adhesion molecules.
Asou
H
H
Department of Physiology, Keio University, School of Medicine, Tokyo, Japan.
Miura
M
M
Kobayashi
M
M
Uyemura
K
K
Itoh
K
K
D000818:Animals; D015816:Cell Adhesion Molecules, Neuronal / Q000502:physiology*; D002465:Cell Movement / Q000502:physiology; D002478:Cells, Cultured; D002531:Cerebellum / Q000166:cytology* / Q000502:physiology; D005455:Fluorescent Antibody Technique; D051379:Mice
D015816:Cell Adhesion Molecules, Neuronal