title: Effects of alkyl alcohols and related chemicals on rat liver structure and function. III. Physiochemical properties of ethanol-, propanol- and butanol- treated rat liver mitochondrial membranes.
abstract: The physicochemical properties of mitochondria in liver tissue obtained from rats given 32% ethanol, 32% propanol or 6.9% butanol in drinking water for up to 3 months were investigated using differential scanning calorimetry and fluorescence polarization measurements. The results obtained were as follows: 1) Phospholipids extracted from mitochondria showed increases in the relative amounts of phosphatidylcholine, phosphatidylinositol and phosphatidylserine, and a decrease in the relative amount of phosphatidylethanolamine. An increase in the unsaturated/saturated fatty acid ratio of phospholipids was also observed. 2) Elevation of the thermotropic lipid phase transition temperature with a decrease in the enthalpy value (delta H) was revealed by differential scanning calorimetry. 3) The elevation of the lipid phase transition temperature was detected also by fluorescence polarization measurements using 1,6-diphenyl-1,3,5-hexatriene (DPH) as a probe. Elevation of mitochondrial membrane fluidity was found in some of the experimental animals, but most showed no changes in comparison with the control. A possible role of membrane fusion in the mechanism of formation of ethanol-, propanol- and butanol-induced hepatic megamitochondria is discussed on the basis of these results.
authors.affiliation: Department Cell Biology and Molecular Pathology, Nagoya University School of Medicine, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Adachi
authors.forename: M
authors.initials: M
authors.lastname: Momota
authors.forename: Y
authors.initials: Y
authors.lastname: Teranishi
authors.forename: R
authors.initials: R
authors.lastname: Ueki
authors.forename: M
authors.initials: M
authors.lastname: Hagiwara
authors.forename: T
authors.initials: T
authors.lastname: Wakabayashi
authors.forename: J
authors.initials: J
authors.lastname: Popinigis
mesh_terms: D000433:1-Propanol / Q000494:pharmacology*; D000818:Animals; D000440:Butanols / Q000494:pharmacology*; D002152:Calorimetry, Differential Scanning; D005591:Chemical Fractionation; D000431:Ethanol / Q000494:pharmacology*; D005454:Fluorescence Polarization; D008099:Liver / Q000187:drug effects* / Q000648:ultrastructure; D008297:Male; D008566:Membranes / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D008930:Mitochondria, Liver / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D010743:Phospholipids / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000440:Butanols; D010743:Phospholipids; D000431:Ethanol; D000433:1-Propanol