The suspension culture of Tripterygium wilfordii.
In suspension culturing of Tripterygium wilfordii, 18.4 mg/l of dry cells and 9.184 mg/l of diterpene-lactone compounds were obtained from 6,7-V liquid medium containing 1 mg/l NAA. Besides those within the cells, diterpene-lactone compounds were also found in the cultured liquid and the yield reached 30.16-43.14% of the total yield. The total yield of diterpene-lactone compounds in suspension culture (6,7-V medium containing 1 mg/l NAA) was 12.19 times as much as that in the roots of the original plant. In addition, 3 samples of diterpene-lactone extracts from the suspension culture were found toxic to KB cells. Compared with those in solid culture, less incubation time was needed and more diterpene-lactone compounds were produced in suspension culture.
Yin
Z
Z
Institute of Materia Medica, Chinese Academy of Medical Sciences, Beijing.
Chu
W
W
D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002855:Chromatography, Thin Layer; D004224:Diterpenes / Q000032:analysis / Q000633:toxicity; D006801:Humans; D007783:Lactones / Q000032:analysis / Q000633:toxicity; D063245:Plant Development; D014407:Tumor Cells, Cultured
D004224:Diterpenes; D007783:Lactones