title: Sequence of the URA1 gene encoding dihydroorotate dehydrogenase from the basidiomycete fungus Agrocybe aegerita.
abstract: The URA1 gene encoding dihydroorotate dehydrogenase (DHOdehase) from the edible basidiomycete, Agrocybe aegerita, has been cloned by complementation of the Escherichia coli pyrD mutation. The nucleotide sequence of a 1531-bp genomic fragment carrying URA1 revealed two uninterrupted open reading frames (ORFs) separated by 61 bp. The larger ORF can encode a 328-amino acid (aa) DHOdehase that has 53% homology with the corresponding protein from E. coli. Comparison with other DHOdehase aa sequences showed essentially conservation of the cofactor-binding site of flavoproteins.
authors.affiliation: Laboratoire de Génétique Moléculaire et Amelioration des Champignons Cultivés, Université de Bordeaux II-INRA, Villenave d'Ornon, France.
authors.forename: T
authors.initials: T
authors.lastname: Noël
authors.forename: J
authors.initials: J
authors.lastname: Labarère
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D001487:Basidiomycota / Q000235:genetics*; D003001:Cloning, Molecular; D004271:DNA, Fungal; D000090722:Dihydroorotate Dehydrogenase; D004926:Escherichia coli; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000235:genetics*; D044925:Oxidoreductases Acting on CH-CH Group Donors
chemical_list: D004271:DNA, Fungal; D000090722:Dihydroorotate Dehydrogenase; D010088:Oxidoreductases; D044925:Oxidoreductases Acting on CH-CH Group Donors