[Induction of monooxygenase system and incorporation of radioactivity from 2-14C-lysine into proteins of rat liver microsomes under the action of phenobarbital and the background of lysine, methionine, threonine and vitamin A, C and E deficiencies].
The effect of diet on induction of monooxygenases and distribution of radioactivity from 2-14-C-lysine in fractions of liver homogenate, muscle homogenate and blood of male rats treated with phenobarbital (80 mg/kg, three days) was studied. 2-14-C-lysine was injected intraperitoneally 24 h before the first injection of phenobarbital. It was demonstrated that monooxygenase induction, increase of relative liver weight and incorporation of radioactivity from 2-14-C-lysine into fractions of liver homogenate in phenobarbital-treated rats fed diet deficient in lysine, methionine, threonine and vitamins A, C and E were more pronounced as compared with the similarly treated rats which were fed a balanced diet. The possibility of mobilization of deficient essential components to liver from other organs and tissues for maintenance of monooxygenase induction is discussed.
Nurmagambetov
T Zh
TZh
Amirov
B B
BB
Kuanysheva
T K
TK
Sharmanov
T Sh
TSh
D000818:Animals; D001361:Avitaminosis / Q000201:enzymology*; D004032:Diet; D066298:In Vitro Techniques; D008239:Lysine / Q000097:blood / Q000172:deficiency* / Q000378:metabolism*; D008297:Male; D008715:Methionine / Q000172:deficiency*; D008862:Microsomes, Liver / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism; D009132:Muscles / Q000201:enzymology; D010634:Phenobarbital / Q000494:pharmacology*; D051381:Rats; D013268:Stimulation, Chemical; D013912:Threonine / Q000172:deficiency*
D013912:Threonine; D008715:Methionine; D008239:Lysine; D010634:Phenobarbital
Induktsiia monooksigenaznoĭ sistemy i vkliuchenie radioaktivnoĭ metki iz 2-14C-lizina v belki mikrosom pecheni krys pri vozdeĭstvii fenobarbitala na fone defitsita lizina, metionina, treonina i vitaminov A, C i E