[The structural-functional changes in the cells of Francisella tularensis strain 15 Gaĭskiĭ during cultivation and sublimational drying].
Instrumental methods of investigation were used for the demonstration of changes in the fatty acid composition of F. tularensis, strain 15 Gaĭskiĭ, during cultivation in solid culture medium, storage after lyophilization, as well as changes in the functioning of the system of membrane-dependent enzymes of the respiratory chain and in the permeability of cell wall membranes by water molecules and NADH after lyophilization. A relationship between the survival rate of F. tularensis cells after lyophilization and stimulation of their endogenic respiration with NADH and succinate was revealed. An increase in residual moisture from 6 to 10-12% was found to intensify the process of lipid peroxidation during the storage of lyophilized F. tularensis cells of strain 15 Gaĭskiĭ.
Klimenko
V V
VV
Eremenko
Iu D
IuD
Mazin
V P
VP
Tikhonravov
V V
VV
Sadovoĭ
N V
NV
D001428:Bacterial Vaccines; D001431:Bacteriological Techniques; D002462:Cell Membrane / Q000502:physiology / Q000648:ultrastructure; D002463:Cell Membrane Permeability / Q000187:drug effects; D003470:Culture Media; D005604:Francisella tularensis / Q000166:cytology* / Q000276:immunology / Q000502:physiology; D005612:Freeze Drying / Q000295:instrumentation / Q000379:methods; D009243:NAD / Q000493:pharmacokinetics; D013997:Time Factors; D014867:Water / Q000378:metabolism
D001428:Bacterial Vaccines; D003470:Culture Media; D014867:Water; D009243:NAD
Strukturno-funktsional'nye izmeneniia kletok Francisella tularensis shtamma 15 Gaĭskogo pri kul'tivirovanii i sublimatsionnom vysushivanii.