[Tubular structures in Mycoplasma gallisepticum and the localization of a tubulin-like protein].
In all the strains of M. gallisepticum investigated, a protein with apparent molecular weight 40 kDa was revealed by immunoblotting with polyclonal anti-calf brain tubulin antibodies and monoclonal anti-chicken alpha-tubulin antibodies. In other 8 investigated Mycoplasma species no positive reactions with the same antibodies were found. The M. Gallisepticum cells were examined under electron microscope on fine serial sections and on some sections going at different angles to the long cell axis. Undermembrane system of tubules was revealed and the intracellular pattern of the tubular structures were reconstructed. The immunoelectron microscopic data suggest that tubulin-like protein may be included into the structures.
Nikonov
A V
AV
Korolev
E V
EV
Snigirevskaia
E S
ES
Brudnaia
M S
MS
Komissarchik
Ia Iu
IaIu
Ivanov
P I
PI
Borkhsenius
S N
SN
D000128:Acholeplasma laidlawii / Q000737:chemistry / Q000648:ultrastructure; D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D015151:Immunoblotting; D008854:Microscopy, Electron; D016253:Microscopy, Immunoelectron; D008870:Microtubules / Q000737:chemistry / Q000648:ultrastructure*; D008970:Molecular Weight; D009174:Mycoplasma / Q000737:chemistry / Q000648:ultrastructure*; D009176:Mycoplasma mycoides / Q000737:chemistry / Q000648:ultrastructure; D014404:Tubulin / Q000032:analysis / Q000648:ultrastructure*
D014404:Tubulin
Tubuliarnye struktury Mycoplasma gallisepticum i lokalizatsiia tubulinopodobnogo belka.