title: A simple method for detection of monoclonal isotypes.
abstract: A simple rapid method of enzyme labeled anti-isotype assay (ELIA) for detection of monoclonal isotype on hybridoma cells is proposed. This alternative method was first carried out on hybridoma cell lines 147C11 and 257C11 produced against Trypanosoma cruzi and male accessory secretion of Panstrongylus megistus, respectively. The monoclonal antibodies produced by these hybridoma were characterized by this method as IgM (147C11) and IgG1 (257C23) isotypes, allowing evaluation of isotype without having to wait until the concentration of antibody present in the supernatant itself rises. Results were confirmed by Ouchterlony immunodiffusion. The proposed method offers the advantages of a permanent rapid procedure for light microscopy.
authors.affiliation: Departamento de Imunologia, Centro de Pesquisas Aggeu Magalhães-FIOCRUZ, Cidade Universitária, Recife-PE, Brazil.
authors.forename: Y M
authors.initials: YM
authors.lastname: Gomes
authors.forename: L N
authors.initials: LN
authors.lastname: Regis
authors.forename: A B
authors.initials: AB
authors.lastname: Carvalho
authors.forename: M
authors.initials: M
authors.lastname: Nakazawa
authors.forename: L
authors.initials: L
authors.lastname: Monjour
authors.forename: A F
authors.initials: AF
authors.lastname: Furtado
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000145:classification* / Q000302:isolation & purification*; D001709:Biotechnology; D005069:Evaluation Studies as Topic; D006825:Hybridomas / Q000276:immunology; D007124:Immunoenzyme Techniques; D007132:Immunoglobulin Isotypes / Q000302:isolation & purification*; D051379:Mice
chemical_list: D000911:Antibodies, Monoclonal; D007132:Immunoglobulin Isotypes