Pulsed field gel electrophoresis of representatives of Mycobacterium tuberculosis and Mycobacterium bovis BCG strains.
Using field inversion gel electrophoresis (FIGE), different Mycobacterium tuberculosis strains, such as phage prototypes, exhibit different DNA restriction patterns which are easy to compare. Virulent and avirulent variants of M. tuberculosis H37, as well as daughter strains of M. bovis BCG, display characteristic DNA profiles. BCG strains isolated from suppurative adenitis following vaccination of French patients showed patterns identical to the BCG Pasteur strain used for vaccination. These results demonstrate that FIGE of DNA restriction fragments generated by DraI represents a suitable technique for the analysis of mycobacteria at a genomic level. The DraI profiles allow the differentiation and precise identification of the BCG Pasteur, Glaxo, Russian and Japanese strains.
Varnerot
A
A
Centre National de Référence pour les Mycobactéries, Unité de la Tuberculose et des Mycobactéries, Paris, France.
Clément
F
F
Gheorghiu
M
M
Vincent-Lévy-Frébault
V
V
D004269:DNA, Bacterial / Q000235:genetics* / Q000302:isolation & purification*; D015252:Deoxyribonucleases, Type II Site-Specific; D016521:Electrophoresis, Gel, Pulsed-Field; D006801:Humans; D009163:Mycobacterium bovis / Q000235:genetics*; D009169:Mycobacterium tuberculosis / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D013045:Species Specificity; D014376:Tuberculosis / Q000382:microbiology; D014774:Virulence / Q000235:genetics
D004269:DNA, Bacterial; D015252:Deoxyribonucleases, Type II Site-Specific; C035594:TTTAAA -specific type II deoxyribonucleases