Changes in probe sensitivity during NMR spectroscopic studies of the perfused rat heart: a warning.
During NMR experiments on isolated, perfused rat heart, we observed a 57% change in probe sensitivity when the perfusion buffer surrounding the heart was replaced by either a low-ionic-strength mannitol solution or air and the heart was made ischemic. Such changes in sensitivity, should they go unrecognized, would result in substantial quantification errors.
Nédélec
J F
JF
Institute for Biological Sciences, National Research Council, Ottawa, Ontario, Canada.
Saunders
J K
JK
Morris
P
P
Clarke
K
K
D000388:Air; D000818:Animals; D002314:Cardioplegic Solutions; D005947:Glucose; D009682:Magnetic Resonance Spectroscopy / Q000295:instrumentation / Q000379:methods*; D008297:Male; D008353:Mannitol; D017202:Myocardial Ischemia / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism*; D063065:Organophosphonates / Q000378:metabolism; D009994:Osmolar Concentration; D010477:Perfusion; D051381:Rats; D017207:Rats, Sprague-Dawley; D014325:Tromethamine
D002314:Cardioplegic Solutions; C074097:Krebs-Henseleit solution; D063065:Organophosphonates; D014325:Tromethamine; D008353:Mannitol; D005947:Glucose