Transcriptional regulation of hexokinase I mRNA levels by TSH in cultured rat thyroid FRTL5 cells.
We investigated the effect of thyroid stimulating hormone (TSH) on the expression of hexokinase I mRNA by cultured rat thyroid FRTL5 cells. TSH stimulated hexokinase I gene expression in a time- and dose-dependent manner. An increase in hexokinase I mRNA was detected after 3 h of incubation with TSH, and a maximum was reached after 12 h showing about 2.5-fold increase at 1 mU/ml TSH. A nuclear run-on transcriptional assay showed that the effect of TSH on hexokinase I mRNA was due to an increase in the rate of gene transcription. (Bu)2cAMP and forskolin also increased hexokinase I mRNA expression to almost the same extent as TSH. These findings suggest that TSH stimulates hexokinase I gene expression at the transcriptional level via the cAMP-dependent pathway.
Yokomori
N
N
Third Department of Internal Medicine, University of Yamanashi Medical School, Tamaho, Japan.
Tawata
M
M
Hosaka
Y
Y
Onaya
T
T
D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002478:Cells, Cultured; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D006593:Hexokinase / Q000187:drug effects* / Q000235:genetics; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000187:drug effects; D051381:Rats; D013961:Thyroid Gland / Q000166:cytology / Q000201:enzymology*; D013972:Thyrotropin / Q000494:pharmacology*; D014158:Transcription, Genetic / Q000187:drug effects
D012333:RNA, Messenger; D013972:Thyrotropin; D006593:Hexokinase