title: [Function, molecular structure and gene expression of interleukin-1].
abstract: Recent cloning of human and murine IL-1 receptor (IL-1R) has revealed that there are at least two type of IL-1R: type I IL-1R is detected on T cells and fibroblasts and consists of 552 AAs with a cytoplasmic domain of 213 AAs, while type II is detected on B cells and monocytic cell lines and consists of 398 AAs with a short stretch intracytoplasmic domain of 29 AAs. Extracytoplasmic portion of IL-1R has some homology with vaccinia virus B15 Ag or fibroblast protein ST-2, while cytoplasmic portion has considerable similarity with Drosophila toll gene. By transfecting murine type I IL-1R cDNA into a human Jurkat cell line, structural and functional potion required for the IL-1 signal transduction is determined. At least broad portion of cytoplasmic domain including 364-474 AAs from N-terminus are found to be essential, while PKC acceptor site (Ser-431 and Ser-509), and PKA acceptor site (Ser-528) are not essential for the IL-8 gene expression.
authors.affiliation: Department of Medical Biology and Parasitology, Jichi Medical School.
authors.forename: T
authors.initials: T
authors.lastname: Kasahara
authors.forename: K
authors.initials: K
authors.lastname: Kuno
authors.forename: K
authors.initials: K
authors.lastname: Matsushima
mesh_terms: D000595:Amino Acid Sequence; D006801:Humans; D007375:Interleukin-1 / Q000737:chemistry / Q000502:physiology*; D008969:Molecular Sequence Data; D015394:Molecular Structure; D017472:Receptors, Interleukin-1 / Q000737:chemistry / Q000502:physiology*; D015398:Signal Transduction
chemical_list: D007375:Interleukin-1; D017472:Receptors, Interleukin-1