Mineralization of 2-chloro- and 2,5-dichlorobiphenyl by Pseudomonas sp. strain UCR2.
Pseudomonas sp. strain UCR2 was isolated from a multi-chemostat mating experiment between a chlorobenzoate-degrader, Pseudomonas aeruginosa strain JB2, and a chlorobiphenyl-degrader, Arthrobacter sp. strain B1Barc. Strain UCR2 differed from either of the parental organisms in that it grew on both 2-chloro- and 2,5-dichlorobiphenyl with concomitant release of chloride. Phenotypic typing by the Biolog system indicated that strain UCR2 shared greater similarity with strain JB2 (88%) than strain B1Barc (3%). In DNA:DNA hybridization experiments, genomic DNA from strain UCR2 hybridized with both strain JB2 and strain B1Barc, with the former pairing yielding a much stronger signal than the latter. In contrast, no hybridization whatsoever was observed when the parental organisms strains JB2 and B1Barc were probed against each other.
Hickey
W J
WJ
Department of Soil and Environmental Science, University of California, Riverside.
Brenner
V
V
Focht
D D
DD
D001673:Biodegradation, Environmental; D001713:Biphenyl Compounds / Q000378:metabolism*; D004269:DNA, Bacterial / Q000235:genetics; D008903:Minerals / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D010101:Oxygen Consumption; D011078:Polychlorinated Biphenyls / Q000378:metabolism*; D011549:Pseudomonas / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
D001713:Biphenyl Compounds; D004269:DNA, Bacterial; D008903:Minerals; C055142:2-chlorobiphenyl; C029905:2,5-dichlorobiphenyl; D011078:Polychlorinated Biphenyls