title: Scattering of the rRNA genes on the physical map of the circular chromosome of Leptospira interrogans serovar icterohaemorrhagiae.
abstract: Leptospira interrogans is a pathogenic bacterium with a low G+C content (34 to 39%). The restriction enzymes NotI, AscI, and SrfI cut the chromosome of L. interrogans serovar icterohaemorrhagiae into 13, 3, and 5 fragments separable by one- and two-dimensional pulsed-field gel electrophoresis (PFGE). The genome is composed of a circular 4.6-Mbp chromosome and a 0.35-Mbp extrachromosomal element. A physical map of the chromosome was constructed for NotI, AscI, and SrfI by using single and double digests, or partial NotI digests obtained at random or by cross-protection of NotI sites by FnuDII methylase, and linking clones. rRNA genes were found to be widely scattered on the chromosome.
authors.affiliation: Unité de Bactériologie Moléculaire et Médicale, Institut Pasteur, Paris, France.
authors.forename: C
authors.initials: C
authors.lastname: Baril
authors.forename: J L
authors.initials: JL
authors.lastname: Herrmann
authors.forename: C
authors.initials: C
authors.lastname: Richaud
authors.forename: D
authors.initials: D
authors.lastname: Margarita
authors.forename: I S
authors.initials: IS
authors.lastname: Girons
mesh_terms: D001483:Base Sequence; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D003001:Cloning, Molecular; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D005798:Genes, Bacterial / Q000235:genetics*; D016680:Genome, Bacterial; D007921:Leptospira interrogans / Q000235:genetics*; D008969:Molecular Sequence Data; D012335:RNA, Ribosomal / Q000235:genetics*; D012703:Serotyping
chemical_list: D012335:RNA, Ribosomal; C078382:endodeoxyribonuclease AscI; C078383:endodeoxyribonuclease SrfI; D015252:Deoxyribonucleases, Type II Site-Specific; C038565:GCGGCCGC-specific type II deoxyribonucleases