[In vitro phenomenon of beta cell migration from fetal calf pseudoislets].
Pseudoislets--pancreatic microfragments containing a lot of beta-cells and capable of insulin secretion in vitro-were obtained from 12 fetal calf pancreata by the use of collagenase. Morphological and functional changes of the pseudoislets were studied during culture. We found a rapid migration of beta-cells out of the pseudoislets to the bottom of plastic tissue culture plates. This process was accompanied by a significant decrease of insulin-secreting capacity of the floating microfragments. This should be taken into consideration in cases when pseudoislets are prepared for transplantation in order to avoid beta-cell loss.
Ablamunits
V G
VG
Kirsanova
L A
LA
D000818:Animals; D002417:Cattle; D002465:Cell Movement; D002478:Cells, Cultured; D007328:Insulin / Q000378:metabolism; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000166:cytology* / Q000378:metabolism; D016381:Islets of Langerhans Transplantation
D007328:Insulin
Fenomen migratsii beta-kletok iz psevdoostrovkov podzheludochnoĭ zhelezy plodov krupnogo rogatogo skota in vitro.