title: In vitro inhibition of struvite crystal growth by acetohydroxamic acid.
abstract: Struvite (MgNH4PO46H2O) crystals were produced by Proteus mirabilis growth in artificial urine, in the presence and absence of the urease inhibitor, acetohydroxamic acid (AHA). In the absence of AHA, struvite crystals assumed an "X-shaped" or dendritic crystal habit due to rapid growth along their 100 axis. When AHA was present, crystal growth, as monitored by phase contrast light microscopy, was greatly slowed, and the crystals assumed an octahedral crystal habit. Scanning electron microscopy revealed that crystals grown in the presence of AHA were pitted on their surface. This pitting was absent in control samples. While most of this inhibition by AHA was due to lowered urease activity, some crystal growth inhibition occurred in struvite produced in the absence of urease activity through NH4OH titration of artificial urine. We conclude that while AHA is primarily a urease inhibitor, it may also disrupt struvite growth and formation directly through interference with the molecular growth processes on crystal surfaces.
authors.affiliation: Department of Urology, Queen's University, Kingston, Canada.
authors.forename: J A
authors.initials: JA
authors.lastname: Downey
authors.forename: J C
authors.initials: JC
authors.lastname: Nickel
authors.forename: L
authors.initials: L
authors.lastname: Clapham
authors.forename: R J
authors.initials: RJ
authors.lastname: McLean
mesh_terms: D000818:Animals; D003460:Crystallization; D006877:Hydroxamic Acids / Q000494:pharmacology*; D008274:Magnesium / Q000378:metabolism*; D017616:Magnesium Compounds; D010710:Phosphates / Q000378:metabolism*; D011513:Proteus mirabilis / Q000254:growth & development / Q000378:metabolism*; D000069877:Struvite; D014556:Urine
chemical_list: D006877:Hydroxamic Acids; D017616:Magnesium Compounds; D010710:Phosphates; C006358:acetohydroxamic acid; D000069877:Struvite; D008274:Magnesium