title: Immunoglobulin G antibody capture enzyme-linked immunosorbent assay: a versatile assay for detection of anti-human immunodeficiency virus type 1 and 2 antibodies in body fluids.
abstract: In tests on specimens of dried blood, saliva, and urine from 55 human immunodeficiency virus (HIV)-seropositive and 55 HIV-seronegative patients, an immunoglobulin G capture enzyme immunoassay for the detection of antibodies to HIV types 1 and 2, GACELISA, gave 109 of 110, 109 of 109, and 109 of 110 correct results, respectively. This performance, achieved in a laboratory previously unfamiliar with the assay, suggests that GACELISA is a useful new epidemiological tool for the study of HIV infection, equally applicable to all three kinds of specimen.
authors.affiliation: Department of Microbiology, Siriraj Hospital, Bangkok, Thailand.
authors.forename: P
authors.initials: P
authors.lastname: Thongcharoen
authors.forename: C
authors.initials: C
authors.lastname: Wasi
authors.forename: S
authors.initials: S
authors.lastname: Louisirirotchanakul
authors.forename: J
authors.initials: J
authors.lastname: Parry
authors.forename: J
authors.initials: J
authors.lastname: Connell
authors.forename: P
authors.initials: P
authors.lastname: Mortimer
mesh_terms: D001826:Body Fluids / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005069:Evaluation Studies as Topic; D005188:False Negative Reactions; D015483:HIV Antibodies / Q000032:analysis*; D006679:HIV Seropositivity / Q000175:diagnosis / Q000276:immunology; D015497:HIV-1 / Q000276:immunology*; D015498:HIV-2 / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G
chemical_list: D015483:HIV Antibodies; D007074:Immunoglobulin G