[The enhancement of the diagnostic reliability of immunoblot for HIV-1 antibodies by enriching the preparation for immunoblot with the HIV-1 gp120 protein].
The diagnostic value of original immunoblot system depends on the availability of enveloped protein GP120 because it is the antibodies to this polypeptide that frequently indicate the running virus infection. This polypeptide is lost during purification of viral material but remains free in culture medium. The extraction of GP120 from culture fluid with immunosorbent based on sepharose 4B with ligated immunoglobulins from HIV-1-infected persons enriched the preparation for immunoblot with proteins increasing its diagnostic value.
Zlobin
A Iu
AIu
Glushchenko
O P
OP
Stakhanova
V M
VM
Uryvaev
L V
LV
D002353:Carrier State / Q000175:diagnosis; D015483:HIV Antibodies / Q000097:blood*; D015699:HIV Envelope Protein gp120 / Q000276:immunology / Q000302:isolation & purification; D015658:HIV Infections / Q000175:diagnosis; D006679:HIV Seropositivity / Q000175:diagnosis; D006801:Humans; D006863:Hydrogen-Ion Concentration; D015151:Immunoblotting / Q000379:methods*; D007163:Immunosorbent Techniques; D015203:Reproducibility of Results
D015483:HIV Antibodies; D015699:HIV Envelope Protein gp120
Uvelichenie diagnosticheskoĭ dostovernosti immunoblota na antitela k VICh-1 za schet obogashcheniia preparata dlia immunoblota belkom gp120 VICh-1.