Immunological demonstration of a unique 3,4-dihydroxyphenylacetate 2,3-dioxygenase in soil Arthrobacter strains.
Many bacteria biosynthesize 3,4-dihydroxyphenylacetate 2,3-dioxygenases for growth on aromatic acids, but gram-negative organisms have been most extensively studied. A gram-positive strain containing 2,3-dioxygenase activity was identified as Arthrobacter strain Mn-1. The 2,3-dioxygenase from strain Mn-1 was purified to homogeneity by fast protein liquid chromatography with a Mono Q anion-exchange column. Rabbit polyclonal antidioxygenase antibodies were prepared. Ouchterlony double-diffusion and Western blotting (immunoblotting) protocols were used to probe the distribution of the Mn-1 dioxygenase antigen in soil bacteria. Fourteen 2,3-dioxygenase-containing Bacillus and Pseudomonas strains did not contain immunologically cross-reactive proteins. Six of eight Arthrobacter strains contained 2,3-dioxygenase activity, and all of them produced cross-reactive proteins. The data presented here suggest that a unique type of dioxygenase is geographically widespread but is taxonomically confined to Arthrobacter soil bacteria.
Olson
P E
PE
Department of Biochemistry, University of Minnesota, Navarre 55392.
Qi
B
B
Que
L
L
Wackett
L P
LP
D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D000907:Antibodies, Bacterial / Q000737:chemistry; D000911:Antibodies, Monoclonal / Q000737:chemistry; D001173:Arthrobacter / Q000145:classification / Q000201:enzymology* / Q000378:metabolism; D001426:Bacterial Proteins / Q000032:analysis* / Q000145:classification / Q000276:immunology; D003429:Cross Reactions; D049308:Dioxygenases; D007120:Immunochemistry; D010105:Oxygenases / Q000145:classification / Q000276:immunology* / Q000302:isolation & purification; D011817:Rabbits; D012988:Soil Microbiology; D013045:Species Specificity
D000596:Amino Acids; D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D001426:Bacterial Proteins; D010105:Oxygenases; D049308:Dioxygenases; C020055:3,4-dihydroxyphenylacetate 2,3-dioxygenase