In vivo phospholipid modification induces changes in microsomal delta 5-desaturase activity.
The phospholipid composition of rat-liver microsomes was modified by feeding weaning rats a choline-free diet. After 21 days, the phosphatidylcholine content decreased with a concomitant increment of phosphatidylserine and cholesterol. The bulk fluidity of the membrane decreased. Under these conditions, the delta 5-desaturase activity was diminished as well as the arachidonic-acid content of the membrane lipids.
Leikin
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Instituto de Investigaciones Bioquímicas de La Plata (INIBIOLP), CONICET-UNLP, Facultad de Ciencias Médicas, Argentina.
Brenner
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RR
D000818:Animals; D002794:Choline / Q000008:administration & dosage; D000090943:Delta-5 Fatty Acid Desaturase; D004032:Diet; D044943:Fatty Acid Desaturases / Q000378:metabolism*; D005454:Fluorescence Polarization; D008297:Male; D008563:Membrane Lipids / Q000378:metabolism; D008862:Microsomes, Liver / Q000201:enzymology*; D010743:Phospholipids / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
D000090943:Delta-5 Fatty Acid Desaturase; D008563:Membrane Lipids; D010743:Phospholipids; D044943:Fatty Acid Desaturases; D002794:Choline