title: Insulin and lipogenesis in rat adipocytes. I. Effect of insulin incubation on lipid synthesis by isolated rat adipocytes.
abstract: To assess the effect of insulin on lipid synthesis in isolated rat adipocytes, cells were preincubated for 3 h with high concentrations (16.6 nM) of the hormone and lipogenesis measured through 14C-acetate incorporation into lipids, analyzing at the same time the activity of some lipogenic enzymes. It was found that insulin induced not only a decrease in the number of insulin receptors but a 30% loss in basal and insulin-stimulated acetate incorporation into total lipids as well as a decrease in the activities of enzymes related to the novo fatty acid synthesis pathway as malic enzyme and glucose-6-phosphate dehydrogenase.
authors.affiliation: Facultad de Ciencias, Universidad del Zulia, Maracaibo, Venezuela.
authors.forename: L M
authors.initials: LM
authors.lastname: Morales
authors.forename: G
authors.initials: G
authors.lastname: Campos
authors.forename: E
authors.initials: E
authors.lastname: Ryder
authors.forename: A
authors.initials: A
authors.lastname: Casanova
mesh_terms: D000085:Acetates / Q000378:metabolism; D000273:Adipose Tissue / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D002250:Carbon Radioisotopes; D002478:Cells, Cultured; D004247:DNA / Q000378:metabolism; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D007328:Insulin / Q000494:pharmacology*; D007700:Kinetics; D008055:Lipids / Q000096:biosynthesis*; D008291:Malate Dehydrogenase / Q000378:metabolism; D008297:Male; D009097:Multienzyme Complexes / Q000378:metabolism; D007652:Oxo-Acid-Lyases / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D011972:Receptor, Insulin / Q000187:drug effects / Q000378:metabolism
chemical_list: D000085:Acetates; D002250:Carbon Radioisotopes; D007328:Insulin; D008055:Lipids; D009097:Multienzyme Complexes; D004247:DNA; D008291:Malate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase; D011972:Receptor, Insulin; D007652:Oxo-Acid-Lyases; C019909:citrate (pro-3S)-lyase