Changes in concentration of essential metals in kidneys and urine as indices of gentamicin nephrotoxicity in female Wistar rats.
Wistar rats were treated with gentamicin in single (80 mg/kg) or repeated doses (7 x 40 mg/kg) subcutaneously. Total protein as well as excretion of essential metals (Cu, Zn) with the urine were determined 24 hr after 1, 3 and 7 dosages as well as 3 and 7 days after the termination of administration. At the same time kidneys were examined histopathologically by light microscopy. Simultaneously, Cu, Zn and metallothionein levels in kidneys and liver were determined. Rats receiving gentamicin demonstrated progressive renal proximal tubular necrosis at the end of 7 days administration. At the same time elevated copper and zinc levels were observed in urine. These essential metals seem to be an indicator of gentamicin nephrotoxicity.
Chmielnicka
J
J
Department of Toxicological Chemistry, Medical Academy in Lódź, School of Pharmacy, Poland.
Szymańska
J A
JA
Brzeźnicka
E A
EA
Kałuzyński
A
A
D000818:Animals; D003300:Copper / Q000032:analysis / Q000652:urine*; D005260:Female; D005839:Gentamicins / Q000633:toxicity*; D007668:Kidney / Q000737:chemistry / Q000187:drug effects* / Q000473:pathology; D007683:Kidney Tubular Necrosis, Acute / Q000139:chemically induced* / Q000378:metabolism / Q000473:pathology; D007687:Kidney Tubules, Proximal / Q000473:pathology; D008668:Metallothionein / Q000032:analysis; D011507:Proteinuria; D051381:Rats; D017208:Rats, Wistar; D015032:Zinc / Q000032:analysis / Q000652:urine*
D005839:Gentamicins; D003300:Copper; D008668:Metallothionein; D015032:Zinc