Phage pattern and antibiotic resistance pattern of coagulase-negative staphylococci obtained from immunocompromised patients.
A total of 152 coagulase-negative staphylococcal strains were isolated from clinical samples of 14 patients hospitalized after bone-marrow transplantation in a specialized hospital ward in Hungary, during an 18-month period between 1987 and 1989. Two species, Staphylococcus epidermidis and Staphylococcus haemolyticus, predominated (each, 45%). Using Pulverer and co-workers' phage set for typing, 68% of the isolates were typable; 16 phage patterns were observed. A characteristic long pattern with phages Ph10/Ph13/Ph15/U4/U15/U16/U20/U33 /U46 appeared only in S. epidermidis, among 5 of 11 colonized patients (8.5% of all strains). Single lysis with phage Ph13 was observed in 7 of the 14 patients (49% of all strains), in species S. capitis, S. epidermidis, S. haemolyticus, S. hominis, and S. warneri. In S. haemolyticus, non-typable strains predominated (66%); this character occurred only in 2% among other species. The strains colonizing the immunocompromised patients differed from each other in phage pattern, antibiotic resistance pattern, and/or slime production. No hospital infection was suggested. On the other hand, high incidence of two well-definable phage patterns raises some relationship between phage receptors or some regulatory systems in phage multiplication and factors responsible for special colonization as common surface properties.
Barcs
I
I
Bacteriophage Department, B. Johan National Institute for Public Health, Budapest, Hungary.
Herendi
A
A
Lipcsey
A
A
Bognár
C
C
Hashimoto
H
H
D015373:Bacterial Typing Techniques; D016026:Bone Marrow Transplantation / Q000009:adverse effects / Q000276:immunology; D002490:Central Nervous System / Q000382:microbiology; D003030:Coagulase / Q000378:metabolism; D004352:Drug Resistance, Microbial; D006801:Humans; D016867:Immunocompromised Host; D013045:Species Specificity; D013210:Staphylococcus / Q000145:classification* / Q000201:enzymology / Q000302:isolation & purification; D013204:Staphylococcus Phages / Q000302:isolation & purification
D003030:Coagulase