Mutations of the Ki-ras oncogene in endometrial carcinoma.
OBJECTIVE
The purpose of this study was to assess the extent of involvement of the ras oncogene in endometrial carcinoma.


METHODS
Genomic deoxyribonucleic acid from 30 samples of endometrial carcinoma was examined for point mutations in codons 12, 13, and 61 from the Ha-ras, Ki-ras, and N-ras genes by means of the polymerase chain reaction, slot-blotting, and deoxyribonucleic acid sequencing procedures.


RESULTS
An apparent somatic mutation of Ki-ras codon 12 in one of 10 paraffin-embedded tumors was confirmed by deoxyribonucleic acid sequence analysis. Two of 20 frozen endometrial carcinoma specimens were also shown to contain a point mutation in Ki-ras codon 12. No correlation between ras mutation and a number of histologic or clinical parameters was observed.


CONCLUSIONS
These data suggest a potential role for Ki-ras codon 12 mutations in the development of some (10%) endometrial cancers.
Ignar-Trowbridge
D
D
Laboratory of Reproductive and Developmental Toxicology, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709.
Risinger
J I
JI
Dent
G A
GA
Kohler
M
M
Berchuck
A
A
McLachlan
J A
JA
Boyd
J
J
D003001:Cloning, Molecular; D003062:Codon; D016889:Endometrial Neoplasms / Q000235:genetics* / Q000473:pathology; D005260:Female; D011905:Genes, ras / Q000235:genetics*; D006801:Humans; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
D003062:Codon; D015345:Oligonucleotide Probes