Alpha 1-proteinase inhibitor and resistance to acute blood loss during injection of beta-1,3-carboxymethylglucan.
The present study was undertaken to evaluate the mechanism of protective effect of semisoluble alpha-1,3-carboxymethylglucan during massive acute hemorrhage. CBA mice were injected i.v. with glucan (25 mg/kg) 24 h prior to hemorrhage (50% of blood circulating volume). Survival data indicated that glucan increased survival compared with the control. However alpha 1-proteinase inhibitor activity in serum has been even decreased 24 h after glucan administration. Moreover enhancement of active oxygen form production and leakage of cytosolic and lysosomal enzymes after alpha-1,3-carboxymethylglucan application was noted.
Vereschagin
E
E
Institute of Physiology, Siberian Branch of the Russian Academy of Medical Sciences, Novosibirsk.
Korolenko
T
T
Arkhipov
S
S
D000818:Animals; D003600:Cytosol / Q000201:enzymology; D005936:Glucans / Q000494:pharmacology*; D006470:Hemorrhage / Q000503:physiopathology* / Q000517:prevention & control; D006867:Hydrolases / Q000097:blood; D008247:Lysosomes / Q000201:enzymology; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D000515:alpha 1-Antitrypsin / Q000378:metabolism*; D047071:beta-Glucans
D005936:Glucans; D000515:alpha 1-Antitrypsin; D047071:beta-Glucans; C029933:carboxymethyl-beta-1,3-glucan; D006867:Hydrolases