[Expression of orthopoxvirus DNA sequences in Escherichia coli cells].
We observed the expression of recombinant plasmids genes containing ectromelia virus DNA fragments in E. coli minicells. Using plasmids with vaccinia or ectromelia viruses DNA fragments inserted upstream of lacZ gene we showed that certain orthopoxvirus genome fragments carry out a promoter-like function in bacterial cells.
Shchelkunov
S N
SN
Riazankina
O I
OI
Krykbaev
R A
RA
Belavin
P A
PA
D001345:Autoradiography; D001483:Base Sequence; D004279:DNA, Viral / Q000235:genetics*; D004481:Ectromelia virus / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics*; D015870:Gene Expression; D005814:Genes, Viral; D008969:Molecular Sequence Data; D010957:Plasmids; D011401:Promoter Regions, Genetic; D014616:Vaccinia virus / Q000235:genetics*
D004279:DNA, Viral
Ekspressiia posledovatel'nosteĭ DNK ortopoksivirusov v kletkakh Escherichia coli.