Consequences of replication fork movement through transcription units in vivo.
To examine the basis for the evolutionary selection for codirectionality of replication and transcription in Escherichia coli, electron microscopy was used to visualize replication from an inducible ColE1 replication origin inserted into the Escherichia coli chromosome upstream (5') or downstream (3') of rrnB, a ribosomal RNA operon. Active rrnB operons were replicated either in the same direction in which they were transcribed or in the opposite direction. In either direction, RNA polymerases were dislodged during replication. When replication and transcription were codirectional, the rate of replication fork movement was similar to that observed in nontranscribed regions. When replication and transcription occurred in opposite directions, replication fork movement was reduced.
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Department of Biology, University of Virginia, Charlottesville 22903.
D004261:DNA Replication; D004269:DNA, Bacterial / Q000648:ultrastructure; D004275:DNA, Ribosomal / Q000235:genetics; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D008854:Microscopy, Electron; D012335:RNA, Ribosomal / Q000235:genetics; D014158:Transcription, Genetic
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012335:RNA, Ribosomal; D012321:DNA-Directed RNA Polymerases; D004259:DNA-Directed DNA Polymerase