title: Bacillary haemoglobinuria diagnosis by the peroxidase-antiperoxidase (PAP) technique.
abstract: A peroxidase-antiperoxidase (PAP) technique was used to diagnose bacillary haemoglobinuria in formalin-fixed, paraffin-embedded liver tissues of cattle. The PAP method revealed Clostridium haemolyticum in the zone of liver necrosis characteristic of the disease and also in culture smears of this microorganism, but C. novyi type B, C. chauvoei, C. septicum and C. perfringens types B and C remained unstained by the PAP reaction. The PAP technique performed provides a specific, simple and rapid method to diagnose bacillary haemoglobinuria.
authors.affiliation: Instituto Nacional de Tecnología Agropecuaria (INTA), Bariloche, Argentina.
authors.forename: F A
authors.initials: FA
authors.lastname: Uzal
authors.forename: K
authors.initials: K
authors.lastname: Belak
authors.forename: E
authors.initials: E
authors.lastname: Rivera
authors.forename: C A
authors.initials: CA
authors.lastname: Robles
authors.forename: R E
authors.initials: RE
authors.lastname: Feinstein
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis*; D003013:Clostridium / Q000302:isolation & purification*; D003015:Clostridium Infections / Q000175:diagnosis / Q000662:veterinary*; D006456:Hemoglobinuria / Q000175:diagnosis / Q000662:veterinary*; D007124:Immunoenzyme Techniques / Q000662:veterinary*; D008099:Liver / Q000382:microbiology