title: Use of synthetic H disaccharides as acceptors for detecting activities of UDP-GalNAc:Fuc alpha 1-->2Gal beta-R alpha 1-->3-N-acetylgalactosaminyltransferase in plasma samples from blood group A subgroups.
abstract: Concentrations of blood group A-specified alpha(1-->3)-N-acetylgalactosaminyltransferase (A enzyme) were measured in human plasma of blood groups A1, A2, and A3 by using chemically synthesized H disaccharides and H type 1 and type 2 trisaccharides attached to hydrophobic aglycones as acceptors. When the trisaccharides were used as acceptors, enzyme activities were reduced in samples from A2 and A3. However, the H disaccharides were shown to be good acceptors even for enzymes from A2 and A3, and no significant difference in enzyme concentration was detected in any of the plasma tested.
authors.affiliation: Department of Legal Medicine, School of Medicine, Gunma University, Maebashi, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Yazawa
authors.forename: A
authors.initials: A
authors.lastname: Takeya
authors.forename: O
authors.initials: O
authors.lastname: Hosomi
authors.forename: T
authors.initials: T
authors.lastname: Nakajima
authors.forename: N
authors.initials: N
authors.lastname: Shimoda
authors.forename: H
authors.initials: H
authors.lastname: Ohi
authors.forename: T
authors.initials: T
authors.lastname: Tachikawa
authors.forename: C F
authors.initials: CF
authors.lastname: Piscorz
authors.forename: R K
authors.initials: RK
authors.lastname: Jain
authors.forename: K L
authors.initials: KL
authors.lastname: Matta
mesh_terms: D000017:ABO Blood-Group System; D002240:Carbohydrate Sequence; D004187:Disaccharides / Q000737:chemistry / Q000378:metabolism*; D006596:Hexosaminidases / Q000378:metabolism; D006801:Humans; D008745:Methylation; D008969:Molecular Sequence Data; D017350:N-Acetylgalactosaminyltransferases / Q000097:blood*; D014312:Trisaccharides / Q000378:metabolism; D001619:beta-N-Acetylhexosaminidases / Q000378:metabolism
chemical_list: D000017:ABO Blood-Group System; D004187:Disaccharides; D014312:Trisaccharides; D017350:N-Acetylgalactosaminyltransferases; C003742:UDPgalactosamine-galactose acetylgalactosaminyltransferase; D006596:Hexosaminidases; D001619:beta-N-Acetylhexosaminidases