The K3 type killer strains of genus Saccharomyces for wine production.
Using simultaneous fusion of protoplasts of three strains the auxotrophic K3 killer designated MH1 was prepared at a very low frequency and further employed for the transfer of the K3 killer character into a commercial wine yeast. A novel Saccharomyces Bratislava 1K3 strain with desirable technological and anti-yeast killer abilities was thus constructed. Attempts to prepare double K1/K3 killers were made. The obtained fusion products contained M1 dsRNA and were able to produce only the K1 type killer toxin.
Sulo
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Department of Biochemical Technology, Faculty of Chemical Technology, Slovak Technical University, Bratislava.
Michalcáková
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D007218:Industrial Microbiology; D011523:Protoplasts; D012331:RNA, Fungal / Q000235:genetics; D012440:Saccharomyces / Q000235:genetics* / Q000472:pathogenicity; D014920:Wine
D012331:RNA, Fungal