Determination of protein concentrations and their molecular weight in tears from cats with normal corneas and cats with corneal sequestrum.
Protein concentration was determined, using the Bradford technique, in tears from cats with normal corneas and from cats with corneal sequestrum. Tears from the former group contained 5.81 +/- 2.29 mg of protein/ml; those from corneal sequestrum-affected cats contained 6.21 +/- 2.21 mg/ml. Difference between the 2 values was not significant. Molecular weight determination was made, using 4 to 20% sodium dodecyl sulfate-polyacrylamide gels. Molecular mass of proteins ranged from 263 to 14 kDa. There was no detectable difference in the band patterns for the 2 groups.
Davidson
H J
HJ
Department of Immunology and Microbiology, Wayne State University School of Medicine, Detroit, MI 48201.
Gerlach
J A
JA
Bull
R W
RW
D000818:Animals; D002371:Cat Diseases / Q000378:metabolism*; D002415:Cats; D003316:Corneal Diseases / Q000378:metabolism / Q000662:veterinary*; D004589:Electrophoresis, Disc / Q000662:veterinary; D005136:Eye Proteins / Q000378:metabolism*; D008970:Molecular Weight; D010865:Pilot Projects; D013666:Tears / Q000737:chemistry*
D005136:Eye Proteins