title: A protein from Daudi cell line conditioned medium induces ovarian dysgenesis in Drosophila melanogaster.
abstract: From a medium in which Daudi cells had been grown, we isolated by HPLC a protein that caused ovarian abnormalities in adult females of Drosophila melanogaster when injected into preblastoderm embryos. This protein, whose apparent M(r) is between 30,000 and 50,000, was found to be a moderately polar compound which is heat stable and whose activity is destroyed by acidification. The protein is characteristic of medium conditioned from Daudi cells.
authors.affiliation: Laboratoire de Physiologie de la Reproduction, Université Pierre et Marie Curie, Paris, France.
authors.forename: N
authors.initials: N
authors.lastname: Richard-Mercier
authors.forename: M
authors.initials: M
authors.lastname: Thomas-Orillard
authors.forename: M
authors.initials: M
authors.lastname: Morinière
authors.forename: P
authors.initials: P
authors.lastname: Porcheron
authors.forename: D
authors.initials: D
authors.lastname: Soyez
authors.forename: M
authors.initials: M
authors.lastname: Fellous
mesh_terms: D000818:Animals; D002452:Cell Count; D002851:Chromatography, High Pressure Liquid; D017077:Culture Media, Conditioned / Q000737:chemistry; D004331:Drosophila melanogaster / Q000033:anatomy & histology*; D005260:Female; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008297:Male; D009363:Neoplasm Proteins / Q000302:isolation & purification / Q000502:physiology*; D010053:Ovary / Q000002:abnormalities; D013696:Temperature; D014407:Tumor Cells, Cultured; D001613:beta 2-Microglobulin / Q000502:physiology
chemical_list: D017077:Culture Media, Conditioned; D009363:Neoplasm Proteins; D001613:beta 2-Microglobulin