title: The actin genes of Onchocerca volvulus.
abstract: The genome of Onchocerca volvulus was found to contain 2 actin gene classes (called 1 and 2) of 2 genes each. The 4 genes are located in 2 clusters (called A and B), each containing a gene class member. Five short introns of 122-207 bp occur within each gene. The sequences of the fourth intron and the 5' and 3' untranslated regions of all the 2 gene classes are completely different even though their coding regions share 95% identity. Mature transcripts from the actin genes have the nematode spliced leader (SL) at their 5' ends. One actin cDNA was found to be derived from an actin pre-mRNA which locks both the 5 introns and the 5' SL, suggesting that in at least some transcripts cis-splicing is completed before trans-splicing occurs.
authors.affiliation: Department of Biochemistry, University of Iowa, Iowa City 52242.
authors.forename: W
authors.initials: W
authors.lastname: Zeng
authors.forename: J E
authors.initials: JE
authors.lastname: Donelson
mesh_terms: D000199:Actins / Q000737:chemistry / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D003062:Codon / Q000235:genetics; D004247:DNA / Q000737:chemistry*; D015723:Gene Library; D017238:Genes, Helminth; D007438:Introns; D008969:Molecular Sequence Data; D005810:Multigene Family; D017181:Onchocerca volvulus / Q000235:genetics*; D012326:RNA Splicing; D012333:RNA, Messenger / Q000737:chemistry; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D014158:Transcription, Genetic
chemical_list: D000199:Actins; D003062:Codon; D012333:RNA, Messenger; D004247:DNA