Development of an irradiated vaccine that protects against enterotoxigenic Escherichia coli diarrhoea.
In the pathogenesis of diarrhoea in man bacteria adhesion to enterocytes is mediated by specific CFA/I or CFA/II antigens. A perorally administered vaccine was prepared from E. coli H10407 (078:H11) by irradiation with electrons with high energy (EHE). Two hours after cimetidine administration rats were immunized per os with 5 irradiated vaccine doses at 4-day intervals. Seven days after the last immunization animals were infected by inoculating 1 x 10(9) germs in the ligated intestinal loop. Reduction of the intestinal secretion by over 50% 18 hours after inoculation was considered an efficient protection marker. The obtained results have proved a significant reduction of the intestinal secretion in immunized animals infected with serotypes 078:H11(63 +/- 4%) and 078:H12(59 +/- 5%) as compared to non-immunized animals. Experimental induction of the intestinal protection against Escherichia coli enterotoxigenic (ETEC) strains points to the possibility of using this type of irradiated vaccine in the prophylaxis of diarrhoea in man.
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Cantacuzino Institute, Bucharest, Romania.
Ionescu
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Popa
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Ionescu
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D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology* / Q000528:radiation effects; D001422:Bacterial Adhesion; D001427:Bacterial Toxins / Q000276:immunology* / Q000528:radiation effects; D001428:Bacterial Vaccines / Q000008:administration & dosage; D003967:Diarrhea / Q000453:epidemiology / Q000382:microbiology / Q000517:prevention & control*; D003968:Diarrhea, Infantile / Q000401:mortality; D004768:Enterotoxins / Q000276:immunology* / Q000528:radiation effects; D004926:Escherichia coli / Q000276:immunology* / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000453:epidemiology / Q000517:prevention & control*; D029968:Escherichia coli Proteins; D005243:Feces / Q000382:microbiology; D006801:Humans; D007223:Infant; D007413:Intestinal Mucosa / Q000378:metabolism / Q000382:microbiology / Q000648:ultrastructure; D051381:Rats; D017208:Rats, Wistar; D015164:Vaccines, Inactivated
D000942:Antigens, Bacterial; D001427:Bacterial Toxins; D001428:Bacterial Vaccines; D004768:Enterotoxins; D029968:Escherichia coli Proteins; D015164:Vaccines, Inactivated; C027538:heat-labile enterotoxin, E coli