Quantitation of paraquat in biological samples by radioimmunoassay using a monoclonal antibody.
We have developed a sensitive and specific radioimmunoassay for the quantitative determination of paraquat in plasma, urine, and bronchoalveolar lavage fluid using a monoclonal antibody. The synthesis of the iodinated paraquat tracer is novel and less complicated than a previously reported method. Regardless of the type of biological sample analyzed, the sensitivity of the assay was 0.46 ng/ml in a 200-microliters aliquot. The results correlated well with those of another assay performed in a separate laboratory. Paraquat concentrations were determined in plasma and in urine of a dog over several days after the intravenous administration of 7.48 mg paraquat cation/kg and in bronchoalveolar lavage fluid obtained 34 hr after the 2-hr infusion was discontinued. Concentrations of paraquat measured ranged from 14.1 to 0.03 micrograms/ml in plasma and 2034 to 0.36 micrograms/ml in urine. Concentrations of paraquat in plasma obtained at various times after the ingestion of paraquat by three patients ranged from 51.0 to 0.010 micrograms/ml.
Bowles
M R
MR
Department of Medicine, University of Queensland, Princess Alexandra Hospital, Brisbane, Australia.
Eyles
D W
DW
Hampson
E C
EC
Pond
S M
SM
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000918:Antibody Specificity; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D002478:Cells, Cultured; D004285:Dogs; D007202:Indicators and Reagents; D007262:Infusions, Intravenous; D007457:Iodine Radioisotopes; D051379:Mice; D008807:Mice, Inbred BALB C; D010269:Paraquat / Q000032:analysis* / Q000276:immunology; D011863:Radioimmunoassay
D000911:Antibodies, Monoclonal; D007202:Indicators and Reagents; D007457:Iodine Radioisotopes; D010269:Paraquat