Effect of endotoxin clearance using whole body rinse-out on some blood-gas and metabolic parameters in rats.
Conscious and unrestrained male Wistar rats were infused over a 10 min period with 60 mg/kg Escherichia coli endotoxin (LD100) and observed for 6 hr. Hypotension, metabolic acidosis, hyperlactemia, hypoglycemia, and elevated oxygen extraction from blood developed in the rats. Another group of rats was subjected to blood substitution with FC-43 emulsion (artificial blood) 30 min after endotoxin application until hematocrit dropped to 3%, after which 6-9 ml of donor blood was exchange transfused with the emulsion. This method resulted in 98% endotoxin clearance, improved blood alkaline reserves, preserved blood glucose, and normalized arterial mixed venous oxygen gradient and O2 extraction from the blood (which implies preserved blood circulation status). Side effects included hypotension, a transitory rise in blood lactate level, and a fall in arterial oxygen content due to the lower hemoglobin (erythrocyte) level.
Voynikov
T I
TI
Department of Pharmacology, Kinki University School of Medicine, Osaka, Japan.
Nikolova
I
I
Yamanishi
Y
Y
Kono
T
T
Suzuki
A
A
D000137:Acid-Base Imbalance / Q000378:metabolism*; D000818:Animals; D001784:Blood Gas Analysis; D001786:Blood Glucose / Q000378:metabolism; D001794:Blood Pressure / Q000502:physiology; D001802:Blood Substitutes / Q000627:therapeutic use*; D004731:Endotoxins / Q000008:administration & dosage / Q000097:blood*; D004926:Escherichia coli; D006400:Hematocrit; D007773:Lactates / Q000097:blood; D008297:Male; D010100:Oxygen / Q000097:blood*; D051381:Rats; D017208:Rats, Wistar; D012772:Shock, Septic / Q000378:metabolism* / Q000628:therapy*
D001786:Blood Glucose; D001802:Blood Substitutes; D004731:Endotoxins; D007773:Lactates; D010100:Oxygen