Lytic effect of Bacillus subtilis elastase on gram-positive and negative bacteria.
Elastase of B. subtilis 6a caused lysis of freshly grown cells of Gram-negative (Proteus vulgaris, Klebsiella pneumoniae, Salmonella typhi and Pseudomonas aeruginosa and Gram-positive (B. subtilis) bacteria. Heat killed and lyophilised Gram-positive and negative bacteria showed higher sensitivity to elastase. Both Gram-negative and Gram-positive bacteria were lysed maximally by elastase at pH 8.0. At this pH, activity of elastase was maximum in Tris-HCl and glycine-NaOH buffers followed by Tris-maleate and cacodylate buffers.
Gupta
M
M
Department of Microbiology, Panjab University, Chandigarh, India.
Kaur
M
M
Gupta
K G
KG
D001412:Bacillus subtilis / Q000201:enzymology*; D001426:Bacterial Proteins / Q000494:pharmacology*; D001433:Bacteriolysis; D006090:Gram-Negative Bacteria / Q000187:drug effects*; D006094:Gram-Positive Bacteria / Q000187:drug effects*; D006863:Hydrogen-Ion Concentration; D010196:Pancreatic Elastase / Q000494:pharmacology*
D001426:Bacterial Proteins; D010196:Pancreatic Elastase