Localization of cloned human DNA sequences and analysis of chromosomal alterations by in situ hybridization.
The in situ hybridization technique was used for the localization on human chromosomes of single-copy and repeated sequences and, in addition, for the characterization of altered human chromosomes. Two anonymous clones, single or low-copy, obtained from a human X chromosome library were localized on the distal part of the long arm and in the paracentromeric region of X chromosome, respectively. A genomic fragment of the single-copy thyroglobulin (TG) gene was used to confirm the localization on the distal part of the long arm of chromosome 8. The localization and distribution on human chromosomes of the glyceraldehyde-3-phosphate dehydrogenase (GAPD) multigene family obtained by in situ hybridization and by somatic cell hybrids were compared. A phosphoglycerate kinase (PGK) c-DNA clone, which detects genic and pseudogenic sequences on the X chromosome, was used for the characterization of three small ring markers present in unrelated female patients.
Patracchini
P
P
Centro di Studi Biochimici delle Patologie del Genoma Umano, Università di Ferrara, Italy.
Aiello
V
V
Marchetti
G
G
Palazzi
P
P
Ferrati
M
M
Calzolari
E
E
Bernardi
F
F
D002869:Chromosome Aberrations; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis*; D005260:Female; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics*; D006801:Humans; D017403:In Situ Hybridization / Q000379:methods*; D010735:Phosphoglycerate Kinase / Q000235:genetics*; D012303:Ring Chromosomes; D013954:Thyroglobulin / Q000235:genetics*
D004247:DNA; D013954:Thyroglobulin; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D010735:Phosphoglycerate Kinase