[Affinity modification of the 40S subparticle from human placenta with derivatives of pAUG and pAUGU3].
Affinity labeling of 40S subunits from human placenta with 4-(N-2-chloroethyl-N-methylamino)benzylmethyl-[32P]phosphoamide s of oligoribonucleotides pAUG and pAUGU3 was studied. Covalent attachment of these derivatives to 40S subunits within the complexes with 40S subunits, formed in the presence of Met-tRNAf.eIF-2.GTP, was detected. Both rRNA and ribosomal proteins were modified. Fragments of 18S rRNA, containing sites of the reagent attachment were identified: 1058-1164 for pAUG derivative and 976-1057--for pAUG and pAUGU3 ones. The data obtained allowed to conclude that the presence of the neighbouring codon at the A-site, regardless of the presence of the tRNA in it, affects significantly the arrangement of the trinucleotide template in the codon-anticodon interaction region. The large subunit does not cause significant alterations in the structural organization of the codon-anticodon interaction region.
Mundus
D A
DA
Malygin
A A
AA
Zenkova
M A
MA
Repkova
M N
MN
Ven'iaminova
A G
AG
Smailov
S K
SK
Li
A V
AV
Iskakov
B K
BK
Vratskikh
L V
LV
Iamkovoĭ
V I
VI
D000345:Affinity Labels; D000926:Anticodon; D015139:Blotting, Southern; D003062:Codon; D004275:DNA, Ribosomal / Q000378:metabolism; D005260:Female; D006801:Humans; D009588:Nitrogen Mustard Compounds / Q000737:chemistry; D009843:Oligoribonucleotides / Q000737:chemistry*; D009943:Organophosphorus Compounds / Q000737:chemistry; D010920:Placenta / Q000648:ultrastructure*; D011247:Pregnancy; D012358:RNA, Transfer, Met / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism*; D013698:Templates, Genetic
D000345:Affinity Labels; D000926:Anticodon; D003062:Codon; D004275:DNA, Ribosomal; D009588:Nitrogen Mustard Compounds; D009843:Oligoribonucleotides; D009943:Organophosphorus Compounds; D012358:RNA, Transfer, Met
Affinnaia modifikatsiia 40S subchastits iz platsenty cheloveka proizvodnymi pAUG i pAUGU3.