Proteoglycan obtained from bovine aorta suppress thrombin-induced platelet aggregation.
Proteoglycan (PG), isolated and purified from bovine aorta (intima-media), consisted of 68.6% chondroitin 4/6-sulfate (CS 4/6-S), 30% dermatan sulfate (DS), 1.4% heparan sulfate (HS), and a trace of hyaluronic acid (HA). PG did not affect platelet aggregation induced by ADP, collagen, and epinephrine, but inhibited that induced by thrombin. Of the standard GAGs investigated, hyaluronic acid (HA) and CS-4/6-S slightly inhibited only thrombin-induced platelet aggregation. However, PG and standard GAGs did not affect the thrombin induced aggregation of washed platelets. The effect of PG after papain digestion on thrombin-induced platelet aggregation was less potent than that before. It is suggested by the results of this study that PG in the aorta inactivates plasma thrombin, probably by inhibiting thrombin activators or potentiating substances which inactivate thrombin and that these effect of PG would be mainly due to PG-DS and partly due to PG-HS.
Nakashima
Y
Y
Second Department of Internal Medicine, University of Occupational and Environmental Health Japan, School of Medicine, Kitakyushu.
Matsushima
T
T
Sadayasu
T
T
Takahara
K
K
Segawa
J
J
Kuroiwa
A
A
D000818:Animals; D001013:Aorta, Thoracic / Q000737:chemistry*; D002417:Cattle; D006025:Glycosaminoglycans / Q000032:analysis / Q000494:pharmacology*; D006801:Humans; D010206:Papain; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology*; D011509:Proteoglycans / Q000494:pharmacology*; D013917:Thrombin / Q000037:antagonists & inhibitors*
D006025:Glycosaminoglycans; D010975:Platelet Aggregation Inhibitors; D011509:Proteoglycans; D013917:Thrombin; D010206:Papain