[Detection of proliferative cells by DNA polymerase a as a proliferation associated marker].
Proliferative cell fractions were measured by flow cytometry in 20 patients with acute leukemia, 4 with chronic myelocytic leukemia in blastic crisis and 7 with malignant lymphoma. The cells were fixed with 2% paraformaldehyde followed by staining with fluorescein isothiocyanate conjugated monoclonal antibody against DNA polymerase a. The DNA polymerase a-positive population was widely distributed in leukemia, from 20.4% to 84.7% in peripheral blood and from 6.5% to 92.5% in the bone marrow. A positive correlation was found between the values in peripheral blood and bone marrow. The values ranged from 66.4% to 88.1% in cells from cases of malignant lymphoma. Cryopreserved cells may be available for measurement of DNA polymerase a because the result obtained in both frozen and fresh cells were essentially the same.
Wakita
A
A
Second Department of Internal Medicine, Nagoya City University Medical School.
Kaneda
T
T
D014408:Biomarkers, Tumor / Q000032:analysis*; D002453:Cell Cycle; D004257:DNA Polymerase II / Q000032:analysis*; D005434:Flow Cytometry; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000175:diagnosis / Q000473:pathology; D015470:Leukemia, Myeloid, Acute / Q000175:diagnosis / Q000473:pathology; D014407:Tumor Cells, Cultured
D014408:Biomarkers, Tumor; D004257:DNA Polymerase II