Effect of calcium-modifying agents on the growth of human gastric carcinoma cells in vitro.
The antiproliferative effects of various calcium-modifying agents were investigated in human AGS gastric carcinoma cells in culture. Variation of the extracellular calcium concentration, achieved by addition of calcium to the growth medium or binding of calcium to the calcium-chelating agent EDTA, appeared to have little influence on growth of the tumor cells. In contrast, the calcium antagonist verapamil, the calcium ionophore A 23.187 and the calmodulin antagonist W 7, agents supposed to interfere with the regulation of the intracellular calcium concentration, all exerted marked growth inhibiting effects. Our results provide evidence for an important role of intracellular calcium-dependent mechanisms in growth regulation of the human gastric adenocarcinoma cell line AGS.
Piontek
M
M
Department of Gastroenterology, University Hospital, Duesseldorf, Germany.
Hengels
K J
KJ
D000001:Calcimycin / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism*; D002147:Calmodulin / Q000037:antagonists & inhibitors*; D002455:Cell Division / Q000187:drug effects*; D004273:DNA, Neoplasm / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004492:Edetic Acid / Q000494:pharmacology*; D006801:Humans; D007700:Kinetics; D013274:Stomach Neoplasms; D013449:Sulfonamides / Q000494:pharmacology*; D014407:Tumor Cells, Cultured; D014700:Verapamil / Q000494:pharmacology*
D002147:Calmodulin; D004273:DNA, Neoplasm; D013449:Sulfonamides; D000001:Calcimycin; C017967:W 7; D004492:Edetic Acid; D014700:Verapamil; D002118:Calcium