title: Soluble enkephalin-degrading enzymes released by lymphomic and erythroleukaemic cell lines.
abstract: The possible existence of soluble proteolytic enzymes released by cells of lymphomic (U937 and 1301) and erythroleukaemic (K562) lines was studied measuring the hydrolysis of 3H-leucine enkephalin in the presence of cell-free supernatants obtained from these lines. Results indicate that leu-enkephalin is rapidly degraded in the presence of these supernatants, and that enkephalin disappearance is paralleled by the formation of peptides that can be interpreted as its hydrolysis fragments. To characterize the factors involved in leu-enkephalin degradation, cell supernatants were analyzed by ion exchange and by steric exclusion chromatography. Data obtained indicate the presence of three groups of proteins active in leu-enkephalin degradation: aminopeptidases, dypeptidylaminopeptidases and dypeptidylcarboxypeptidases. In all three lines, these enzymes are represented by a considerable number of distinct activities. The sizable number of soluble enzymes identified and the significant total activity observed suggest a possible role in the regulatory degradation of informational peptides, as proposed by several groups for the membrane-bound proteolytic enzymes of immunocompetent cells.
authors.affiliation: Dipartimento di Medicina Sperimentale e Scienze Biochimiche, II Università degli Sudi di Roma, Tor Vergata, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Marini
authors.forename: A
authors.initials: A
authors.lastname: Urbani
authors.forename: L
authors.initials: L
authors.lastname: Bongiorno
authors.forename: L G
authors.initials: LG
authors.lastname: Roda
mesh_terms: D000595:Amino Acid Sequence; D000626:Aminopeptidases / Q000378:metabolism*; D002268:Carboxypeptidases / Q000378:metabolism*; D002474:Cell-Free System / Q000201:enzymology; D002852:Chromatography, Ion Exchange; D004743:Enkephalin, Leucine / Q000378:metabolism*; D006801:Humans; D004915:Leukemia, Erythroblastic, Acute / Q000201:enzymology*; D008223:Lymphoma / Q000201:enzymology*; D008969:Molecular Sequence Data; D012995:Solubility; D014407:Tumor Cells, Cultured
chemical_list: D004743:Enkephalin, Leucine; D002268:Carboxypeptidases; D000626:Aminopeptidases