Uniparental cytogamy: a novel method for bringing micronuclear mutations of Tetrahymena into homozygous macronuclear expression with precocious sexual maturity.
A new method of inducing self-fertilization, uniparental cytogamy, yields homozygous germinal and somatic genotypes in the ciliate Tetrahymena thermophila. Progeny are highly fertile and show a marked tendency for precocious sexual maturity. This method is highly effective in protocols designed to generate and express nonlethal dominant or recessive mutations.
Cole
E S
ES
Department of Genetics and Development, Cornell University, Ithaca, New York 14853.
Bruns
P J
PJ
D000818:Animals; D002467:Cell Nucleus / Q000648:ultrastructure; D003470:Culture Media; D004351:Drug Resistance; D015870:Gene Expression; D006720:Homozygote; D016296:Mutagenesis; D009154:Mutation; D009994:Osmolar Concentration; D011003:Ploidies; D016808:Tetrahymena thermophila / Q000235:genetics* / Q000254:growth & development; D013997:Time Factors
D003470:Culture Media