[Detection of the deletion of interferon-alpha and beta genes in lymphoblastoid cells by PCR].
The HuIFN-alpha and beta genes were examined by the PCR method in the 11 human lymphoblastoid cell lines. The results showed that the homozygous deletion of HuIFN-alpha and beta genes was detected in 5 of 11 cell lines and in 5 of 11 cell lines, respectively. The deletions of both the HuIFN-alpha and beta genes were observed in 4 of 11 cell lines. One T cell leukemia cell line deleted only HuIFN-alpha gene, while the other T cell leukemia line deleted only HuIFN-beta gene. This suggests that the deletions of HuIFN-alpha and beta genes may be related the development of leukemia or lymphoma.
Kita
M
M
Département de Microbiologie, Université Médicale de la Préfecture de Kyoto, Japan.
Tong
L J
LJ
Nakajima
E
E
Yamada
A
A
Sasaki
O
O
Yamaguchi
N
N
Kishida
T
T
Imanishi
J
J
D002051:Burkitt Lymphoma / Q000235:genetics / Q000473:pathology*; D002460:Cell Line; D002872:Chromosome Deletion; D006801:Humans; D016898:Interferon-alpha / Q000235:genetics*; D016899:Interferon-beta / Q000235:genetics*; D008214:Lymphocytes; D016133:Polymerase Chain Reaction; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics / Q000473:pathology*
D016898:Interferon-alpha; D016899:Interferon-beta
Détection de délétions des gènes des interférons-alpha et beta dans les cellules lymphoblastoïdes humaines par PCR.