title: A simplified gelatin tolerance test to evaluate gastric and pancreatic proteolytic activities.
abstract: To evaluate gastric, pancreatic and intestinal proteolytic activities, a gelatin tolerance test was standardized, and its clinical significance was evaluated in 16 normal subjects and 25 patients who had undergone gastrectomy or had a pancreatic disease. A 15g gelatin dose given to normal subjects resulted in an increase of serum-free and peptide-bound hydroxyproline (HYP), with the peak values being observed at 1 hr and 2 hr, respectively. In patients who had malabsorption, both peak levels of serum-free and peptide-bound HYP were decreased, and the peak value of free HYP was more frequently lower than that of peptide-bound HYP. The determination of serum free HYP after oral administration og gelatin was significantly correlated with BT-PABA test. These results indicate that usefulness of determining serum-free HYP for the gelatin tolerance test as a simple reliable test for digestion and absorption of proteins.
authors.affiliation: First Department of Internal Medicine, Shimane Prefectural Central Hospital, Izumo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Imaoka
authors.forename: T
authors.initials: T
authors.lastname: Suou
authors.forename: C
authors.initials: C
authors.lastname: Hirayama
mesh_terms: D000284:Administration, Oral; D000328:Adult; D000368:Aged; D005260:Female; D005750:Gastric Juice / Q000378:metabolism; D005767:Gastrointestinal Diseases / Q000378:metabolism*; D005780:Gelatin / Q000493:pharmacokinetics*; D006801:Humans; D006909:Hydroxyproline / Q000097:blood; D007408:Intestinal Absorption / Q000187:drug effects; D008297:Male; D008875:Middle Aged; D010182:Pancreatic Diseases / Q000378:metabolism*; D010189:Pancreatic Juice / Q000378:metabolism; D010447:Peptide Hydrolases / Q000378:metabolism
chemical_list: D005780:Gelatin; D010447:Peptide Hydrolases; D006909:Hydroxyproline