Development of insulin sensitivity in rat skeletal muscle. Studies of glucose transporter and insulin receptor mRNA levels.
Expression of GLUT-4 and insulin receptor mRNAs was investigated in rat skeletal muscle by Northern hybridization. GLUT-4 mRNA was barely detectable in foetal muscle, was expressed at low levels by 1-8 days and at 2-3-fold higher levels during and after weaning (18-40 days). In contrast there was little change in insulin receptor mRNA levels prior to weaning and a reduction in mRNA abundance between 18 and 40 days. Weaning rats on to a diet rich in fat prevented the increase in GLUT-4 abundance seen between 15 and 29 days in animals weaned on a high-carbohydrate diet.
Wallace
S
S
Rowett Research Institute, Bucksburn, Aberdeen, UK.
Campbell
G
G
Knott
R
R
Gould
G W
GW
Hesketh
J
J
D000375:Aging; D000818:Animals; D000831:Animals, Newborn; D000833:Animals, Suckling; D015152:Blotting, Northern; D051275:Glucose Transporter Type 4; D007328:Insulin / Q000494:pharmacology*; D009004:Monosaccharide Transport Proteins / Q000096:biosynthesis*; D009124:Muscle Proteins; D009132:Muscles / Q000737:chemistry* / Q000187:drug effects; D012333:RNA, Messenger / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D011972:Receptor, Insulin / Q000096:biosynthesis*; D014886:Weaning
D051275:Glucose Transporter Type 4; D007328:Insulin; D009004:Monosaccharide Transport Proteins; D009124:Muscle Proteins; D012333:RNA, Messenger; C495694:Slc2a4 protein, rat; D011972:Receptor, Insulin