Nuclear localization of estrogen receptor-immunoreactivity in the preoptic area of female rats and its reduction by intraventricular colchicine treatment.
The subcellular localization of estrogen receptor (ER) was investigated in the preoptic area of ovariectomized female rats by electron microscopic immunohistochemistry, using a monoclonal antibody to ER. ER-immunoreactivity was localized in the nuclei of neurons of the periventricular preoptic nucleus (Pe) and the medial preoptic area (MPA). ER-immunoreactivity had a speckled pattern in the nucleus, but was not observed in the nucleolus or cytoplasm. After intraventricular colchicine treatment, ER-immunoreactivity within the nucleus was reduced drastically in neurons of the Pe and the MPA. The possible mechanism by which colchicine alters ER-immunoreactivity is mentioned.
Yuri
K
K
Department of Anatomy, Kyoto Prefectural University of Medicine, Japan.
Kawata
M
M
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D001345:Autoradiography; D002467:Cell Nucleus / Q000378:metabolism*; D003078:Colchicine / Q000008:administration & dosage / Q000494:pharmacology*; D005260:Female; D007276:Injections, Intraventricular; D009474:Neurons / Q000378:metabolism / Q000648:ultrastructure; D010052:Ovariectomy; D011301:Preoptic Area / Q000276:immunology / Q000378:metabolism* / Q000648:ultrastructure; D051381:Rats; D017208:Rats, Wistar; D011960:Receptors, Estrogen / Q000276:immunology / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D011960:Receptors, Estrogen; D003078:Colchicine