Electron-impact and chemical ionization detection of nicotine and cotinine by gas chromatography-mass spectrometry in rat plasma and brain.
Nicotine and its metabolite, cotinine, were measured in rat plasma and brain by gas chromatography-mass spectrometry. Both agents were extracted from plasma and brain, separated on a capillary column, and quantified by single-ion monitoring. The major fragment ions of nicotine and cotinine at m/z 84 and m/z 98, respectively, were monitored by electron-impact ionization detection and the protonated molecular ions at m/z 163 and m/z 177, respectively, were monitored by chemical ionization detection. Both compounds were quantified using deuterium-labeled nicotine and cotinine, respectively, as internal standards.
Deutsch
J
J
Laboratory of Neurosciences, National Institute on Aging, National Institutes of Health, Bethesda, MD 20892.
Hegedus
L
L
Greig
N H
NH
Rapoport
S I
SI
Soncrant
T T
TT
D000818:Animals; D001923:Brain Chemistry; D003367:Cotinine / Q000032:analysis* / Q000097:blood* / Q000493:pharmacokinetics; D004355:Drug Stability; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D008297:Male; D009538:Nicotine / Q000032:analysis* / Q000097:blood* / Q000493:pharmacokinetics; D051381:Rats; D011916:Rats, Inbred F344
D009538:Nicotine; D003367:Cotinine