[Clinical usefulness of the measurement of type-II procollagen carboxypeptide (C-II propeptide, pColl-II-C) in synovial fluid as a marker of collagen metabolism in cartilage].
Type-II procollagen-C-peptide (pColl-II-C) in synovial fluids was studied in 319 patients with osteoarthritis (OA; 151), rheumatoid arthritis (RA; 141), traumatic arthritis (TA; 27) and 15 healthy volunteers using the newly developed ELISA kit. The mean levels of pColl-II-C in synovial fluids of healthy controls, OA, TA, and RA were 0.3 +/- 0.1 ng/ml, 5.9 +/- 0.3 ng/ml, 6.8 +/- 1.4 ng/ml and 1.1 +/- 0.1 ng/ml, respectively. pColl-II-C levels in synovial fluids of OA and TA were significantly higher compared to those of healthy controls and RA. It was also demonstrated that pColl-II-C levels could reflect the quantitative and qualitative change of cartilage metabolism. Therefore, the quantification of this molecule in synovial fluid could be beneficial to know the synthetic activity of type II collagen of chondrocytes, since pColl-II-C is a part of the precursor molecule of type II collagen.
Shinmei
M
M
Department of Orthopaedic Surgery, National Defense Medical College, Saitama.
Nagaya
I
I
Miyanaga
Y
Y
Iwata
H
H
Yamamoto
S
S
Torisu
T
T
Fujii
K
K
Fukubayashi
T
T
Kondo
M
M
Kurosaka
M
M
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001172:Arthritis, Rheumatoid / Q000378:metabolism; D015415:Biomarkers / Q000032:analysis; D002135:Calcium-Binding Proteins / Q000032:analysis*; D002356:Cartilage / Q000378:metabolism*; D003094:Collagen / Q000032:analysis* / Q000378:metabolism*; D024043:Collagen Type II; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010003:Osteoarthritis / Q000378:metabolism; D010446:Peptide Fragments / Q000032:analysis*; D011347:Procollagen / Q000032:analysis*; D013582:Synovial Fluid / Q000737:chemistry*
D015415:Biomarkers; D002135:Calcium-Binding Proteins; D024043:Collagen Type II; D010446:Peptide Fragments; D011347:Procollagen; C040072:chondrocalcin; D003094:Collagen