title: Functional/metabolic modulation of the brain stem lesions caused by 1,3-dinitrobenzene in the rat.
abstract: To determine whether neuronal activity plays a role in the localisation of brain stem lesions in 1,3-dinitrobenzene intoxication we produced asymmetrical changes in auditory input by rupturing the left tympanic membrane in Fischer rats. This raised the auditory threshold on that side from 57-63 dB to 104-122 dB. It also decreased glucose utilisation in the ipsilateral cochlear nucleus and significantly increased utilisation in the contralateral nucleus, resulting in a relative deficit of 72 +/- 6%. Similarly, tympanic membrane rupture led to decreased glucose utilisation in the contralateral and increased utilisation in the ipsilateral inferior colliculus. Additional exposure to "white noise" prevented the decrease in glucose utilisation in the contralateral inferior colliculus. Dosing with dinitrobenzene (10 mg/kg in 4 doses over 48 hr) to otherwise normal rats produces symmetrical vasculonecrotic lesions in these regions, but in animals with left tympanic membrane rupture the severity of morphological changes in the ipsilateral cochlear nucleus and the contralateral inferior colliculus were substantially reduced. Additional exposure to "white noise" increased the degree of damage in the ipsilateral cochlear nucleus and contralateral inferior colliculus. These findings indicate that altered auditory function in rats, with its associated metabolic consequences exercises a significant role in the development of brain stem damage in auditory pathways following dinitrobenzene intoxication.
authors.affiliation: Toxicology Unit, M.R.C. Laboratories, Carshalton, Surrey, United Kingdom.
authors.forename: D E
authors.initials: DE
authors.lastname: Ray
authors.forename: A W
authors.initials: AW
authors.lastname: Brown
authors.forename: J B
authors.initials: JB
authors.lastname: Cavanagh
authors.forename: C C
authors.initials: CC
authors.lastname: Nolan
authors.forename: H K
authors.initials: HK
authors.lastname: Richards
authors.forename: S P
authors.initials: SP
authors.lastname: Wylie
mesh_terms: D000161:Acoustic Stimulation; D000818:Animals; D001309:Auditory Threshold / Q000502:physiology*; D001786:Blood Glucose / Q000378:metabolism; D001933:Brain Stem / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D004136:Dinitrobenzenes / Q000506:poisoning*; D005404:Fixatives; D008297:Male; D009622:Noise; D051381:Rats; D011916:Rats, Inbred F344; D012421:Rupture; D013048:Specimen Handling / Q000379:methods; D014432:Tympanic Membrane / Q000293:injuries*
chemical_list: D001786:Blood Glucose; D004136:Dinitrobenzenes; D005404:Fixatives; C017906:3-dinitrobenzene