Action of a novel kinin precursor, Met-T-kinin-Leu, on prostaglandin I2 and blood pressure.
The novel kinin precursor, Met-T-kinin-Leu, stimulated the release of prostaglandin I2 from endothelial cells cultured using bovine carotid artery endothelial cells and minimum essential medium supplemented with 10% fetal calf serum. However, it failed to stimulate the release of prostaglandin I2 from the cells in the fetal calf serum-free medium conditions. To examine the discrepancy of the release of prostaglandin I2 from the cells by Met-T-kinin-Leu in the presence or absence of fetal calf serum, the products formed from Met-T-kinin-Leu by incubation of culture medium were analyzed by reverse-phase HPLC. Regarding the blood pressure reaction of Met-T-kinin-Leu, it showed from one fifth to one hundredth of that compared with bradykinin in blood pressure reactivity of each species, such as rats, rabbits and cats.
Handa
H
H
Tonan Hospital, Sapporo, Japan.
Goto
K
K
Kanayasu
T
T
Murota
S
S
Izumi
H
H
Suzuki
S
S
Fujie
K
K
Sakamoto
W
W
D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D001920:Bradykinin / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D002339:Carotid Arteries; D002415:Cats; D002417:Cattle; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D003470:Culture Media; D016895:Culture Media, Serum-Free; D004305:Dose-Response Relationship, Drug; D004730:Endothelium, Vascular / Q000187:drug effects / Q000378:metabolism*; D011464:Epoprostenol / Q000378:metabolism*; D010446:Peptide Fragments / Q000302:isolation & purification; D011817:Rabbits; D051381:Rats
D003470:Culture Media; D016895:Culture Media, Serum-Free; D010446:Peptide Fragments; C078646:bradykinin-Leu, Met-Ile-Ser-; D011464:Epoprostenol; D001920:Bradykinin