A lock-docking oligo(dT) primer for 5' and 3' RACE PCR.
We describe a method that can be used to obtain and sequence 3' and 5' ends of cDNA transcripts directly from PCR products. The method employs a modified oligo(dT) primer that enables it to "lock-dock" at the junction of gene-specific cDNA sequence and a natural (3') or appended (5') poly(A) tail. As a result, discrete, first-round PCR products are obtained that are easily isolated and sequenced directly.
Borson
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ND
Department of Biochemistry and Molecular Biology, School of Medicine, University of Minnesota, Duluth 55812.
Salo
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Drewes
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LR
D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000235:genetics; D004285:Dogs; D051274:Glucose Transporter Type 3; D008969:Molecular Sequence Data; D009004:Monosaccharide Transport Proteins / Q000235:genetics; D009419:Nerve Tissue Proteins; D011071:Poly T; D016133:Polymerase Chain Reaction / Q000379:methods*
D051274:Glucose Transporter Type 3; D009004:Monosaccharide Transport Proteins; D009419:Nerve Tissue Proteins; D011071:Poly T; D004247:DNA