Transcription induction of c-Ki-ras with the tumour promoter 12-O-tetradecanoylphorbol-13-acetate (TPA) in normal and transformed liver cells.
Results from nuclear run-off assays show that exposure of hepatocytes and Reuber H35B hepatoma cells to the tumour promoter, 12-O-tetradecanoylphorbol-13-acetate (TPA), leads to enhanced transcription of c-Ki-ras gene. This increase in transcription in turn results in an accumulation of the functionally active c-Ki-ras message. The half life of c-Ki-ras message in both normal and transformed livers cells is not altered by TPA and is determined to be 3.5 hr. The induction of c-Ki-ras message is accompanied by an increase in the level of c-Ki-ras protein.
Chan
S O
SO
Department of Biochemistry, University of Hong Kong.
Wong
S S
SS
Yeung
D C
DC
D000818:Animals; D002273:Carcinogens / Q000494:pharmacology*; D002461:Cell Line, Transformed; D002467:Cell Nucleus / Q000187:drug effects; D011905:Genes, ras / Q000235:genetics*; D006207:Half-Life; D008114:Liver Neoplasms, Experimental / Q000235:genetics*; D014176:Protein Biosynthesis / Q000187:drug effects; D011518:Proto-Oncogene Proteins / Q000235:genetics; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D012016:Reference Values; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D014158:Transcription, Genetic / Q000187:drug effects*
D002273:Carcinogens; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D013755:Tetradecanoylphorbol Acetate