Application of gas chromatography-mass spectrometry for rapid detection of Mycobacterium xenopi in drinking water.
Gas chromatography-mass spectrometry (GC-MS) was used to detect 2-docosanol, a secondary alcohol characteristic of Mycobacterium xenopi, in 7 of 10 analyzed drinking water samples culture positive for that species. GC-MS was also used to detect tuberculostearic acid. Both of these chemical markers were analyzed as halogenated derivatives in the negative-ion-chemical-ionization mode. The numbers of CFU of M. xenopi were lowest in the three GC-MS-negative samples. The described GC-MS method is useful for the rapid detection of M. xenopi in drinking water.
Alugupalli
S
S
Department of Medical Microbiology, University of Lund, Sweden.
Larsson
L
L
Slosárek
M
M
Jaresova
M
M
D003604:Czechoslovakia; D005233:Fatty Alcohols / Q000302:isolation & purification*; D008401:Gas Chromatography-Mass Spectrometry; D009161:Mycobacterium / Q000737:chemistry / Q000254:growth & development / Q000302:isolation & purification*; D014871:Water Microbiology; D014881:Water Supply
D005233:Fatty Alcohols; C529236:docosanol