title: Karyotypic analysis of gastric carcinoma cell lines carrying an amplified c-met oncogene.
abstract: MKN 45 is a poorly differentiated gastric carcinoma cell line from which the subclone GTL 16 was obtained. Both lines carry an amplification unit derived from chromosome 7 sequences and containing an activated c-met oncogene. Karyotypic analysis showed that GTL 16 derived from a subclone of MKN 45 after endoreduplication. Several clonal abnormalities are evident in both lines; some are frequently observed in gastrointestinal tumors (loss of 17p and monosomy 18). Other consistent anomalies include 6q-, t(8;10) and t(5;8), and inv(16). A marker chromosome (M1), which was previously shown to contain the c-met amplification unit, is constantly duplicated in all GTL 16 metaphases; in contrast, most unidentified markers are retained in only a single copy in GTL 16 cells. These data are in agreement with the hypothesis that the c-met oncogene activation in these gastric cancer cell lines might be related to a gene dosage effect.
authors: Department of Biomedical Sciences and Oncology, University of Turin, Italy.
authors: G
authors: G
authors: Rege-Cambrin
authors: P
authors: P
authors: Scaravaglio
authors: F
authors: F
authors: Carozzi
authors: S
authors: S
authors: Giordano
authors: C
authors: C
authors: Ponzetto
authors: P M
authors: PM
authors: Comoglio
authors: G
authors: G
authors: Saglio
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics*; D002869:Chromosome Aberrations; D002872:Chromosome Deletion; D006801:Humans; D007621:Karyotyping; D009006:Monosomy; D009857:Oncogenes; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D019859:Proto-Oncogene Proteins c-met; D013274:Stomach Neoplasms / Q000235:genetics*; D014178:Translocation, Genetic; D014314:Trisomy; D014407:Tumor Cells, Cultured
chemical_list: D011518:Proto-Oncogene Proteins; D011505:Protein-Tyrosine Kinases; D019859:Proto-Oncogene Proteins c-met