Slices from the rat olfactory bulb maintained in vitro. Morphological aspects.
Transverse, 400-microns-thick slices of 8-day-old rat olfactory bulb were incubated in Krebs-Henseleit medium with and without oxygenation. Following incubation, slices were fixed in aldehyde-osmium and embedded in resin for light and electron microscopy. After 2 h of incubation oxygenated preparations showed a structural preservation comparable to that of the freshly fixed olfactory bulb. Under hypoxic conditions mitral cells located on the medial side of the bulb were the most sensitive to the interruption of gassing, while ventricular cells and glomeruli were remarkably resistant as judged by morphological standards. The effects of short-term (up to 30 min) interruptions of gassing proved to be reversible. Our findings suggest that the incubated olfactory bulb slice may be a useful preparation for functional morphological studies.
Hajós
F
F
Department of Anatomy and Histology, University of Veterinary Science, Budapest, Hungary.
Gerics
B
B
Sótonyi
P
P
D000818:Animals; D006651:Histocytochemistry; D066298:In Vitro Techniques; D008854:Microscopy, Electron; D009830:Olfactory Bulb / Q000033:anatomy & histology*; D051381:Rats; D017208:Rats, Wistar