Functional analysis of a CArG-like promoter element in cardiac myosin light chain 2 gene.
We have examined the functional properties of a putative regulatory sequence, CCAAAAGTGG, (element A) in chicken cardiac myosin light chain 2 (MLC2) gene promoter by deletion/substitution mutagenesis and transcriptional analysis of RNA by S1 nuclease mapping. The results indicate that the element A sequence, which resembles the evolutionarily conserved A/T-rich CArG box, plays a role in defining the transcription initiation site in MLC2 gene. This is accomplished via repression of a potential transcription initiation at site -40 and promoting the initiation at +1. One of the two other dA-dT-rich sequences (element C), located proximal to initiation site (+1), serves as the basal promoter while the distal A/T rich element B participates in tissue specific transcription of the gene.
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Department of Anatomy and Cell Biology, State University of New York, Brooklyn 11203.
Danishefsky
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Gadot
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Siddiqui
M A
MA
D000818:Animals; D001483:Base Sequence; D005075:Biological Evolution; D002460:Cell Line; D002645:Chickens; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics / Q000378:metabolism; D004261:DNA Replication; D005091:Exons; D017353:Gene Deletion; D006321:Heart / Q000502:physiology*; D015151:Immunoblotting; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009218:Myosins / Q000235:genetics*; D009687:Nuclear Proteins / Q000378:metabolism; D011401:Promoter Regions, Genetic; D051381:Rats; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic; D014162:Transfection
D009687:Nuclear Proteins; D004247:DNA; D009218:Myosins