The amino-acid sequence of two isoforms of alpha-lactalbumin from donkey (Equus asinus) milk is identical.
The complete primary structure of donkey alpha-lactalbumin A and B has been determined by sequencing of the peptides obtained after tryptic, Glu-C proteinase or cyanogen bromide cleavage. Although preparative purification of alpha-lactalbumin by flat-bed isoelectric focusing gave two protein fractions A and B, their amino-acid sequence revealed no differences. Donkey alpha-lactalbumin shows two, four and five differences in comparison to the horse alpha-lactalbumin A, B and C. Thus donkey alpha-lactalbumin is homogeneous and belongs to the horse A-type variant.
Giufrida
M G
MG
Centro Studi Alimentazione Animali, CNR, Torino, Italy.
Cantisani
A
A
Napolitano
L
L
Conti
A
A
Godovac-Zimmermann
J
J
D000595:Amino Acid Sequence; D000818:Animals; D007768:Lactalbumin / Q000737:chemistry*; D008892:Milk / Q000737:chemistry*; D008969:Molecular Sequence Data; D010527:Perissodactyla; D017386:Sequence Homology, Amino Acid
D007768:Lactalbumin