TAP1 mutant mice are deficient in antigen presentation, surface class I molecules, and CD4-8+ T cells.
The transporter associated with the antigen processing 1 (TAP1) gene encodes a subunit for a transporter, presumed to be involved in the delivery of peptides across the endoplasmic reticulum membrane to class I molecules. We have generated mice with a disrupted TAP1 gene using embryonic stem cell technology. TAP1-deficient mice are defective in the stable assembly and intracellular transport of class I molecules and consequently show severely reduced levels of surface class I molecules. These properties are strikingly similar to those described for the TAP2 mutant cell line RMA-S. Cells from the TAP1-deficient mice are unable to present cytosolic antigens to class I-restricted cytotoxic T cells. As predicted from the near absence of class I surface expression, TAP1-deficient mice lack CD4-8+ T cells.
Van Kaer
L
L
Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge 02139.
Ashton-Rickardt
P G
PG
Ploegh
H L
HL
Tonegawa
S
S
D000818:Animals; D001483:Base Sequence; D015704:CD4 Antigens / Q000235:genetics*; D016827:CD8 Antigens / Q000235:genetics*; D002460:Cell Line; D002678:Chimera; D005786:Gene Expression Regulation; D005805:Genes, MHC Class I / Q000235:genetics*; D051379:Mice; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D013601:T-Lymphocytes / Q000378:metabolism
D015704:CD4 Antigens; D016827:CD8 Antigens