Determination of beta-carotene in plasma, blood cells and buccal mucosa by electrochemical detection.
The beta-carotene concentrations in plasma, blood cells and buccal mucosal cells were determined by high-performance liquid chromatography with electrochemical detection. This method was 1,000 times more sensitive than the conventional spectrophotometric method. Polymorphonuclear cells and red blood cells had lower beta-carotene levels than the other cells. After oral administration of 580 mg/day of all-trans beta-carotene to human male volunteers for a week, the beta-carotene concentrations in all cell types increased at least several times above the original levels.
Murata
T
T
Department of Pediatrics, Osaka Medical College, Japan.
Tamai
H
H
Morinobu
T
T
Manago
M
M
Takenaka
A
A
Takenaka
H
H
Mino
M
M
D000328:Adult; D001792:Blood Platelets / Q000737:chemistry; D002338:Carotenoids / Q000032:analysis* / Q000097:blood* / Q000493:pharmacokinetics; D002610:Cheek; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004563:Electrochemistry; D004912:Erythrocytes / Q000737:chemistry; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000737:chemistry; D008297:Male; D009061:Mouth Mucosa / Q000737:chemistry*; D009504:Neutrophils / Q000737:chemistry; D019207:beta Carotene
D019207:beta Carotene; D002338:Carotenoids