title: Antibodies in the serum of patients with autoimmune thyroid disorders react with a recombinant 98 amino acid fragment of a full length 64 kDa eye muscle membrane protein which is also expressed in the thyroid.
abstract: We have tested sera from patients with autoimmune thyroid disorders with or without ophthalmopathy for immunoreactivity, in a dot blot assay, against a recombinant 98 amino acid fragment of a cloned 64 kDa protein, D1, which is expressed in human eye muscle and thyroid, in the form of a Lac Z fusion protein. Tests were positive in 19 out of 40 patients with established thyroid-associated ophthalmopathy (TAO), in 12 out of 21 patients with Graves' hyperthyroidism (GH) without clinically evident ophthalmopathy, in 5 out of 10 patients with thyroid autoimmunity and lid retraction but no other signs of ophthalmopathy, in 4 out of 23 patients with Hashimoto's thyroiditis (HT) without evident ophthalmopathy and in 2 out of 18 patients with benign adenoma or multinodular goitre, but in only 2 out of 37 normal subjects tested. SDS-polyacrylamide gel electrophoresis and Western blotting for an antibody reactive with a 64 kDa antigen in pig eye muscle membranes was also carried out on sera from patients with TAO and GH. While immunoblotting for antibodies reactive with a 64 kDa protein was more often positive in patients with TAO, in whom 58% had serum antibodies which reacted with a 64 kDa protein, this was not the case in patients with GH without eye signs in whom the prevalence of positive immunoblot tests was 35%. Overall there was a fairly close correlation between the two tests although there were many exceptions.(ABSTRACT TRUNCATED AT 250 WORDS)
authors: Institute de Recherche Interdisciplinaire en Biologie Humaine et Nucléaire, Université libre de Bruxelles.
authors: Z G
authors: ZG
authors: Zhang
authors: Q
authors: Q
authors: Dong
authors: P
authors: P
authors: Rodien
authors: L
authors: L
authors: Alcalde
authors: N
authors: N
authors: Bernard
authors: A
authors: A
authors: Boucher
authors: M
authors: M
authors: Salvi
authors: B
authors: B
authors: Arthurs
authors: G M
authors: GM
authors: Vassart
authors: M
authors: M
authors: Ludgate
mesh_terms: D000328:Adult; D000368:Aged; D000818:Animals; D000918:Antibody Specificity; D001323:Autoantibodies / Q000276:immunology*; D001324:Autoantigens / Q000276:immunology*; D001327:Autoimmune Diseases / Q000209:etiology / Q000276:immunology*; D005128:Eye Diseases / Q000209:etiology / Q000276:immunology*; D005136:Eye Proteins / Q000276:immunology*; D005260:Female; D015870:Gene Expression; D006111:Graves Disease / Q000097:blood / Q000276:immunology*; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000276:immunology*; D008875:Middle Aged; D009124:Muscle Proteins / Q000276:immunology*; D010446:Peptide Fragments / Q000276:immunology*; D011993:Recombinant Fusion Proteins / Q000276:immunology; D013552:Swine / Q000276:immunology; D013961:Thyroid Gland / Q000737:chemistry / Q000276:immunology; D013967:Thyroiditis, Autoimmune / Q000097:blood / Q000150:complications / Q000276:immunology*
chemical_list: D001323:Autoantibodies; D001324:Autoantigens; D005136:Eye Proteins; D008565:Membrane Proteins; D009124:Muscle Proteins; D010446:Peptide Fragments; D011993:Recombinant Fusion Proteins