Molecular cloning and nucleotide sequence of cDNA encoding a functional murine low-density-lipoprotein receptor.
The low-density-lipoprotein (LDL) receptor is an important mediator of mammalian cholesterol metabolism; its congenital absence in humans is characterized by hypercholesterolemia, atherosclerosis, and coronary artery disease. We report here the identification and cloning of a cDNA encoding the murine LDL receptor. The cDNA insert is 4467 base pairs in length and the deduced amino acid sequence bears 78.2% homology with the reported human sequence. This murine cDNA was subcloned into a retroviral-based expression vector, LmLSN1, and transfected into 3T3 cells. The production of functional LDL receptor was confirmed by ligand binding of DiI-LDL cholesterol.
Polvino
W J
WJ
Molecular Hematology Branch, National Heart Lung and Blood Institute, Bethesda, Maryland 20892.
Dichek
D A
DA
Mason
J
J
Anderson
W F
WF
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D005796:Genes; D005822:Genetic Vectors; D007438:Introns; D008077:Lipoproteins, LDL / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D011973:Receptors, LDL / Q000235:genetics* / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D012190:Retroviridae; D017386:Sequence Homology, Amino Acid
D008077:Lipoproteins, LDL; D011973:Receptors, LDL; D011994:Recombinant Proteins; D004247:DNA