title: Analysis of Creutzfeldt-Jakob disease infectious fractions by gel permeation chromatography and sedimentation field flow fractionation.
abstract: Gel permeation chromatography and sedimentation field flow fractionation (SF3) were used to further analyze highly infectious fractions from Creutzfeldt-Jakob disease (CJD) infected hamster brain. These analyses defined the relative molecular mass and physical size of the Creutzfeldt-Jakob disease (CJD) agent with greater precision than previously possible. Highly purified disaggregated fractions yielded single, homogeneous Gaussian peaks with both methods. The relevant analytical peaks contained protein-nucleic acid complexes with an M(r) of approximately 1.5 x 10(7) daltons and a mean radius of approximately 30 nm. The experimental evidence further solidifies the concept of an infectious agent that resembles a viral core rather than a simple protein.
authors: Yale University, School of Medicine, New Haven, CT 06510.
authors: T
authors: T
authors: Sklaviadis
authors: R
authors: R
authors: Dreyer
authors: L
authors: L
authors: Manuelidis
mesh_terms: D000818:Animals; D001921:Brain / Q000382:microbiology*; D001923:Brain Chemistry; D002499:Centrifugation, Density Gradient; D002851:Chromatography, High Pressure Liquid; D007562:Creutzfeldt-Jakob Syndrome / Q000382:microbiology*; D006224:Cricetinae; D008970:Molecular Weight; D009696:Nucleic Acids / Q000032:analysis; D011328:Prions / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D009696:Nucleic Acids; D011328:Prions