The induction of ornithine decarboxylase by ornithine takes place at post-transcriptional level in perifused Ehrlich carcinoma cells.
The increase in ODC activity during perifusion of Ehrlich carcinoma cells with 0.5 mM ornithine correlates with an increase in 'de novo' synthetized ODC protein. ODC synthesis was followed by immunoprecipitation of equal quantities of 35S-labelled proteins after 10, 20 and 30 min of labelling. In addition, the rate of 'de novo' protein synthesis is very much elevated in cells perifused with saline buffer supplemented with 0.5 mM ornithine than in cells perifused with the saline buffer only. In spite of the higher specific ODC activity observed in cells perifused with saline buffer plus 0.5 mM ornithine respect to cells perifused with only saline buffer for 3.5 h, no elevation in ODC mRNA was observed when the cells were perifused in the presence of 0.5 mM ornithine.
Sánchez-Jiménez
F
F
Laboratory of Biochemistry and Molecular Biology, Facultad de Ciencias, Universidad de Málaga, Spain.
Urdiales
J L
JL
Matés
J M
JM
Núñez de Castro
I
I
D000818:Animals; D015152:Blotting, Northern; D002286:Carcinoma, Ehrlich Tumor / Q000201:enzymology* / Q000235:genetics; D004790:Enzyme Induction / Q000235:genetics; D009952:Ornithine / Q000494:pharmacology; D009955:Ornithine Decarboxylase / Q000096:biosynthesis* / Q000187:drug effects; D012333:RNA, Messenger / Q000032:analysis; D012334:RNA, Neoplasm / Q000032:analysis; D013268:Stimulation, Chemical; D014407:Tumor Cells, Cultured
D012333:RNA, Messenger; D012334:RNA, Neoplasm; D009952:Ornithine; D009955:Ornithine Decarboxylase