Enantiospecific gas chromatographic-mass spectrometric procedure for the determination of ketoprofen and ibuprofen in synovial fluid and plasma: application to protein binding studies.
A method for the enantiospecific quantitation of two commonly prescribed non-steroidal anti-inflammatory drugs (ketoprofen and ibuprofen) is described. The method involves formation of a mixed anhydride of the drug with ethylchloroformate and subsequent conversion to an amide by reaction with optically active amphetamine. The subsequently formed diastereomers are separated by gas chromatography-mass spectrometry using selected-ion monitoring. The assay is capable of quantifying ketoprofen (2 ng/ml) and ibuprofen (3 ng/ml) enantiomers from a 200-microliters sample of synovial fluid or plasma and is particularly suitable for protein binding studies.
Jack
D S
DS
School of Pharmacy, University of Tasmania, Hobart, Australia.
Rumble
R H
RH
Davies
N W
NW
Francis
H W
HW
D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D007052:Ibuprofen / Q000032:analysis* / Q000097:blood; D007660:Ketoprofen / Q000032:analysis* / Q000097:blood; D011485:Protein Binding; D012016:Reference Values; D015203:Reproducibility of Results; D013237:Stereoisomerism; D013582:Synovial Fluid / Q000737:chemistry*
D007660:Ketoprofen; D007052:Ibuprofen