Distribution of calbindin D-28K and parvalbumin immunoreactivities in the nucleus olfactorius anterior of the rat.
The distributions of calbindin D-28K (CaBP) and parvalbumin (PV) in the rat nucleus olfactorius anterior (NOA) were described using monoclonal antibodies and the avidin-biotin-peroxidase method. The NOA showed a high immunoreactivity for CaBP, with a rostrocaudal increase in the positive neurons and fibres. Pars externa (NOAe) was the only subdivision which showed a low CaBP immunostaining. PV-positive elements were less abundant than those CaBP immunostained. The main difference in the distributions for both proteins was observed in the pars medialis which was practically PV negative. PV- and CaBP-stained neurons showed similar morphologies in the subdivisions where they were present. In NOAe, we observed a characteristic PV- and CaBP-positive neuronal type, with an oriented dendritic pattern. Transition areas were clearly observable in both CaBP- and PV-labelled sections.
García-Ojeda
E
E
Departamento de Biología Celular y Patología, Facultad de Biología, Universidad de Salamanca, Spain.
Alonso
J R
JR
Arévalo
R
R
Briñón
J G
JG
Lara
J
J
Aijón
J
J
D000818:Animals; D000911:Antibodies, Monoclonal; D064026:Calbindins; D005260:Female; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D009412:Nerve Fibers / Q000648:ultrastructure; D009474:Neurons / Q000166:cytology*; D009830:Olfactory Bulb / Q000033:anatomy & histology / Q000166:cytology*; D010320:Parvalbumins / Q000032:analysis*; D051381:Rats; D017208:Rats, Wistar; D064030:S100 Calcium Binding Protein G / Q000032:analysis*
D000911:Antibodies, Monoclonal; D064026:Calbindins; D010320:Parvalbumins; D064030:S100 Calcium Binding Protein G