Cloning and partial nucleotide sequence of Schistosoma japonicum paramyosin: a potential vaccine candidate against schistosomiasis.
Paramyosin from the blood fluke, Schistosoma mansoni, has shown promise as a vaccine candidate for schistosomiasis mansoni. Here we report the cloning and partial nucleotide sequence of a cDNA encoding paramyosin from the related human parasite, Schistosoma japonicum. Affinity purified antibodies to this clone recognized a S. japonicum antigen of molecular weight 97 kDa, equivalent to the reported size of S. mansoni paramyosin. Alignment of the cDNA sequence with that of S. mansoni paramyosin revealed 90% identity. Comparison of the predicted amino acid sequences revealed 95% identity. Although these two parasites differ in many characteristics, the substantial homology demonstrated here between S. mansoni and S. japonicum paramyosin could have important implications for the development of a S. japonicum vaccine.
Yang
W
W
Molecular Helminthology Laboratory, Queensland Institute of Medical Research, Bancroft Centre, Brisbane, Australia.
Waine
G J
GJ
Sculley
D G
DG
Liu
X
X
McManus
D P
DP
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000737:chemistry; D006801:Humans; D008969:Molecular Sequence Data; D012549:Schistosoma japonicum / Q000235:genetics / Q000276:immunology*; D012554:Schistosomiasis japonica / Q000517:prevention & control*; D014335:Tropomyosin / Q000737:chemistry / Q000235:genetics*; D014612:Vaccines / Q000737:chemistry / Q000235:genetics*
D014335:Tropomyosin; D014612:Vaccines; D004247:DNA