APHE-1 and APHE-2, two new antimicrobial and cytotoxic antibiotics. II. Physico-chemical properties and structure elucidation.
Two new pyrazolo-isoquinolinone antibiotics, APHE-1 and APHE-2, have been isolated from the culture filtrate and mycelia of Streptoverticillium griseocarneum NCIMB 40447. Molecular formulae were established as C13H12N2O for APHE-1 and C14H14N2O for APHE-2, by elemental analysis, NMR and mass spectra. 2D NMR techniques (1H-1H COSY-45 and 1H-13C correlated spectroscopy) have been applied to establish their structures.
Fidalgo
M L
ML
Departamento de Microbiología y Parasitología, Universidad de Alcalá de Henares, Madrid, Spain.
Arias
M S
MS
Soliveri
J
J
Arias
M E
ME
D000900:Anti-Bacterial Agents / Q000737:chemistry*; D000903:Antibiotics, Antineoplastic / Q000737:chemistry*; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D007546:Isoquinolines / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D015394:Molecular Structure; D011720:Pyrazoles / Q000737:chemistry*; D013306:Streptomycetaceae / Q000378:metabolism*
D000900:Anti-Bacterial Agents; D000903:Antibiotics, Antineoplastic; D007546:Isoquinolines; D011720:Pyrazoles; C078848:antibiotic APHE 1; C078851:antibiotic APHE 2