title: Disulfide bond assignment in human J chain and its covalent pairing with immunoglobulin M.
abstract: The assignment of disulfide bonds in human J chain and its covalent pairing with immunoglobulin M was determined under conditions which minimize disulfide bond interchange. We show that in J chain the three intradisulfide bridges are formed between Cys 12 and 100, Cys 71 and 91, and Cys 108 and 133. Previous reports [reviewed by Koshland, M. E. (1985) Annu. Rev. Immunol. 3, 425-453] have proposed that cysteines 12, 14, or 68 were linked to the penultimate cysteine 575 of two mu chain tails. In this work, we demonstrate that cysteines 14 and 68 are disulfide-bridged to mu chains. A revised, albeit putative, model of J chain folding is presented which takes into account the correct disulfide pairing and the predictive secondary structure assignment.
authors: Department of Medical Biochemistry, University of Geneva Medical Center, Switzerland.
authors: S
authors: S
authors: Frutiger
authors: G J
authors: GJ
authors: Hughes
authors: N
authors: N
authors: Paquet
authors: R
authors: R
authors: Lüthy
authors: J C
authors: JC
authors: Jaton
mesh_terms: D000595:Amino Acid Sequence; D015153:Blotting, Western; D003488:Cyanogen Bromide; D004220:Disulfides / Q000737:chemistry*; D006801:Humans; D007133:Immunoglobulin Joining Region / Q000737:chemistry*; D007075:Immunoglobulin M / Q000737:chemistry*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D017433:Protein Structure, Secondary
chemical_list: D004220:Disulfides; D007133:Immunoglobulin Joining Region; D007075:Immunoglobulin M; D003488:Cyanogen Bromide