title: Characterization of a novel variant of amino acid transport system asc in erythrocytes from Przewalski's horse (Equus przewalskii).
abstract: In thoroughbred horses, red blood cell amino acid transport activity is Na(+)-independent and controlled by three codominant genetic alleles (h, l, s), coding for high-affinity system asc1 (L-alanine apparent Km for influx at 37 degrees C congruent to 0.35 mM), low-affinity system asc2 (L-alanine Km congruent to 14 mM), and transport deficiency, respectively. The present study investigated amino acid transport mechanisms in red cells from four wild species: Przewalski's horse (Equus przewalskii), Hartmann's zebra (Zebra hartmannae), Grevy's zebra (Zebra grevyi), and onager (Equus hemonius). Red blood cell samples from different Przewalski's horses exhibited uniformly high rates of L-alanine uptake, mediated by a high-affinity asc1-type transport system. Mean apparent Km and Vmax values (+/- SE) for L-alanine influx at 37 degrees C in red cells from 10 individual animals were 0.373 +/- 0.068 mM and 2.27 +/- 0.11 mmol (L cells.h), respectively. As in thoroughbreds, the Przewalski's horse transporter interacted with dibasic as well as neutral amino acids. However, the Przewalski asc1 isoform transported L-lysine with a substantially (6.4-fold) higher apparent affinity than its thoroughbred counterpart (Km for influx 1.4 mM at 37 degrees C) and was also less prone to trans-stimulation effects. The novel high apparent affinity of the Przewalski's horse transporter for L-lysine provides additional key evidence of functional and possible structural similarities between asc and the classical Na(+)-dependent system ASC and between these systems and the Na(+)-independent dibasic amino acid transport system y+. Unlike Przewalski's horse, zebra red cells were polymorphic with respect to L-alanine transport activity, showing high-affinity or low-affinity saturable mechanisms of L-alanine uptake. Onager red cells transported this amino acid with intermediate affinity (apparent Km for influx 3.0 mM at 37 degrees C). Radiation inactivation analysis was used to estimate the target size of system asc in red cells from Przewalski's horse. The transporter's in situ apparent molecular weight was 158,000 +/- 2500 (SE).
authors: School of Applied Sciences, Wolverhampton Polytechnic, United Kingdom.
authors: D A
authors: DA
authors: Fincham
authors: J C
authors: JC
authors: Ellory
authors: J D
authors: JD
authors: Young
mesh_terms: D000409:Alanine / Q000097:blood / Q000493:pharmacokinetics; D000596:Amino Acids / Q000097:blood* / Q000494:pharmacology; D000818:Animals; D001693:Biological Transport, Active; D004912:Erythrocytes / Q000378:metabolism*; D005260:Female; D005954:Glucosephosphate Dehydrogenase / Q000097:blood; D006736:Horses / Q000097:blood*; D007700:Kinetics; D008239:Lysine / Q000097:blood / Q000493:pharmacokinetics; D008297:Male; D008970:Molecular Weight; D010527:Perissodactyla / Q000378:metabolism*; D012964:Sodium / Q000493:pharmacokinetics / Q000502:physiology
chemical_list: D000596:Amino Acids; D012964:Sodium; D005954:Glucosephosphate Dehydrogenase; D008239:Lysine; D000409:Alanine