[Protection of cultured hippocampal neurons in hypoxia and subsequent reoxygenation by antioxidant from the spatially hindered phenols class].
The influence of antioxidant from hindered phenols (U-18) on a hypoxic neurodestructive effect in mouse hippocampal cell cultures was studied. Morphological and biochemical quantitative analysis of neuronal damage showed that U-18 attenuates nerve cell death resultant from 6-7-hour hypoxia and subsequent 12-hour posthypoxic reoxygenation. This antidestructive action of U-18 was observed upon its introduction in nutrient medium both before and immediately after hypoxic period. Thus, our results suggest that peroxidant reactions play a pivotal role in hypoxic neuronal injury and probably participate in this neurodestructive process mainly during posthypoxic reoxygenation period.
Khaspekov
L G
LG
Erin
A N
AN
Viktorov
I V
IV
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D016923:Cell Death; D015687:Cell Hypoxia; D002478:Cells, Cultured; D006624:Hippocampus / Q000187:drug effects*; D002534:Hypoxia, Brain / Q000517:prevention & control*; D051379:Mice; D009474:Neurons / Q000187:drug effects*; D010100:Oxygen / Q000008:administration & dosage*; D010636:Phenols / Q000494:pharmacology*; D013997:Time Factors
D000975:Antioxidants; D010636:Phenols; D010100:Oxygen
Zashchita kul'tiviruemykh neĭronov gippokampa pri gipoksii i posleduiushcheĭ reoksigenatsii antioksidantom klassa prostranstvenno-zatrudnennykh fenolov.