Rapid and simple method for the determination of alpha 1-acid glycoprotein in serum by column liquid chromatography.
A rapid and simple method for the determination of alpha 1-acid glycoprotein (AAG) in serum was developed by using an anion-exchange column for clean-up of serum and a hydroxyapatite column for high-performance liquid chromatography (HPLC). A good correlation was observed between this HPLC method and the conventional radial immunodiffusion method. The method may also be used to determine the AAG concentration in the serum of experimental animals.
Kishino
S
S
Department of Pharmacy, Hokkaido University Hospital, School of Medicine, Hokkaido University, Sapporo, Japan.
Di
Z S
ZS
Sugawara
M
M
Iseki
K
K
Miyazaki
K
K
Kakinoki
S
S
Homura
A
A
Kitabatake
A
A
D000328:Adult; D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009961:Orosomucoid / Q000032:analysis*
D009961:Orosomucoid