title: PCR detection of structurally abnormal Y chromosomes.
abstract: Three probes each detecting a locus on the proximal long arm of the Y chromosome were partially sequenced. Thus, 3 sets of novel primers were developed which enable PCR detection of these 3 loci. Five previously reported primer sets, 3 on the short arm and each one on the centromere and the distal long arm, were mapped along with the novel three using a mapping panel consisted of 8 patients each with different structural abnormality of the Y chromosome. Now, PCR detection of these 8 loci covering an entire length of the Y chromosome has become possible enabling rapid screening of patients with Y chromosome aberrations.
authors: Department of Congenital Abnormalities Research, National Children's Medical Research Center, Tokyo, Japan.
authors: S
authors: S
authors: Nagafuchi
authors: S
authors: S
authors: Seki
authors: Y
authors: Y
authors: Nakahori
authors: T
authors: T
authors: Tamura
authors: H
authors: H
authors: Numabe
authors: Y
authors: Y
authors: Nakagome
mesh_terms: D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D005260:Female; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D016133:Polymerase Chain Reaction / Q000379:methods*; D012729:Sex Chromosome Aberrations / Q000235:genetics*; D014998:Y Chromosome
chemical_list: D009838:Oligodeoxyribonucleotides