[Identification and characterisation of 200 strains of Brucella canis under test from China].
200 cultures of Brucella canis under test were received from some provinces in our laboratory. 167 (85.59%) of them were identified as Brucella according to morphology, growth properties and serologies. And then, they were proved as Brucella canis with R-serum agglutination and lysed test of phage R/C. Some strains also were performed DNA homologous determination and observation of electron microscopic morphology. The results found out that the genetic constitution and picture of them identified with that of reference strains. Non-agglutination reaction was 4.75% of all strains in R-serum test. 72.49% of them appeared typic reaction in dye sensitivity test. 22.75% was resisted to both thionin and fuchsin, which separated difficulty with R phase strains of Brucella melitensis lysed by phage R/C.
Jian
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National Institute for the Control of Pharmaceutical and Biological Products, Beijing.
D000818:Animals; D002002:Brucella / Q000145:classification / Q000302:isolation & purification* / Q000648:ultrastructure; D002681:China; D004285:Dogs / Q000382:microbiology*