Secondary structure at the beginning of the poly(A) sequence of mouse beta-actin messenger RNA.
A portion of the beta-actin mRNA of mammalian cells is believed to lack a poly(A) tail, because of its failure to bind to oligo(dT)-cellulose. S1 mapping and Northern blot analysis of this mRNA shows it to contain a poly(A) sequence of about 60 nucleotides. Only about 20-40 nucleotides are available for interaction with oligo(dT). The rest is masked, presumably by base-pairing with a poly(U) stretch present in the 3' non-coding region of the mRNA. A similar configuration occurs in the bulk of the actin mRNA, which carries a poly(A) tail with sizes ranging from approximately 60 to 200 nucleotides.
Bandyopadhyay
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R
Department of Biochemistry, Tufts University Health Sciences Schools, Boston, MA 02111.
Brawerman
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G
D000199:Actins / Q000235:genetics*; D000818:Animals; D015152:Blotting, Northern; D051379:Mice; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D011061:Poly A / Q000737:chemistry*; D012333:RNA, Messenger / Q000737:chemistry*; D012510:Sarcoma 180; D014407:Tumor Cells, Cultured
D000199:Actins; D009838:Oligodeoxyribonucleotides; D012333:RNA, Messenger; C027903:oligo (dT); D011061:Poly A