A method for embedding thin membranes in historesin.
A method is described for flat-embedding thin membranous tissues in Historesin. It allows easy orientation for sectioning large areas parallel to the surface. Selected fields can be monitored from the unfixed specimen, throughout preparation, to mounting on the microscope slide. For cross-sectioning, the flat-embedded tissue can be stacked and re-embedded to increase the amount of material examined per section.
Shimotsuma
M
M
John Curtin School of Medical Research, Australian National University, Canberra, ACT.
Schoefl
G I
GI
D000818:Animals; D006801:Humans; D008566:Membranes / Q000737:chemistry; D008689:Methacrylates; D008867:Microtomy / Q000379:methods*; D009852:Omentum / Q000098:blood supply; D010477:Perfusion; D012756:Sheep; D016610:Tissue Embedding / Q000379:methods*
D008689:Methacrylates; C005044:hydroxyethyl methacrylate