Molecular epidemiology of respiratory syncytial virus: rapid identification of subgroup A lineages.
Methods for the rapid analysis of samples of respiratory syncytial (RS) virus are described using the polymerase chain reaction (PCR) followed by restriction mapping. Isolates (either clinical samples or tissue culture grown virus) can readily be divided into subgroups and then further classified into lineages. These methods enable examination of large numbers of isolates by molecular techniques, thereby facilitating research into the molecular epidemiology of the virus.
Cane
P A
PA
Department of Biological Sciences, University of Warwick, Coventry, UK.
Pringle
C R
CR
D001483:Base Sequence; D004279:DNA, Viral / Q000235:genetics; D004812:Epidemiologic Methods; D005069:Evaluation Studies as Topic; D005814:Genes, Viral; D006801:Humans; D007223:Infant; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification; D012136:Respiratory Syncytial Viruses / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D010253:Respirovirus Infections / Q000453:epidemiology; D015183:Restriction Mapping; D014773:Virology / Q000379:methods*
D004279:DNA, Viral; D012367:RNA, Viral