In vitro transfer of N,N'-diacetylchitobiose to glycoproteins in rat liver nuclei.
This work demonstrates that (N-acetyl[14C]glucosamine)2 is transferred from dolichyl pyrophosphate-(N-acetyl[14C]glucosamine)2 to endogenous nuclear glycoproteins. The (N-acetyl[14C]glucosamine)2 moiety is N-linked, since it can be released from the tryptic glycopeptides by N-glycosidase F and by hydrazinolysis, but not by beta-elimination. The biological significance of this direct transfer of N,N'-diacetylchitobiose to nuclear proteins remains to be elucidated.
Frot-Coutaz
J
J
Laboratoire de biochimie des membranes, UM 24, Université Claude Bernard-Lyon, Villeurbanne, France.
Degiuli
A
A
Martel
M B
MB
Létoublon
R
R
D000818:Animals; D002240:Carbohydrate Sequence; D002467:Cell Nucleus / Q000378:metabolism*; D004187:Disaccharides; D004288:Dolichol Phosphates / Q000378:metabolism; D005936:Glucans / Q000378:metabolism*; D006023:Glycoproteins / Q000378:metabolism*; D006031:Glycosylation; D016695:Glycosyltransferases / Q000378:metabolism*; D008099:Liver / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000378:metabolism*; D009844:Oligosaccharides / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D051381:Rats; D017208:Rats, Wistar
D004187:Disaccharides; D004288:Dolichol Phosphates; D005936:Glucans; D006023:Glycoproteins; D009687:Nuclear Proteins; D009844:Oligosaccharides; C010913:N,N-diacetylchitobiose; C026406:dolichol pyrophosphate; D016695:Glycosyltransferases