title: The cloning and nucleotide sequence of the serine protease gene (aspA) of Aeromonas salmonicida ssp. salmonicida.
abstract: The gene for Aeromonas salmonicida serine protease has been cloned into phagemid pTZ18R in two restriction fragments, 2.0-kb PstI and 2.3-kb KpnI, of genomic DNA. The nucleotide sequences of the two fragments have been determined, in both directions, after subcloning, by double-stranded sequencing of nested deletions. An open reading frame of 1863 bp translated into a sequence of 621 amino acids, a 24-amino acid signal peptide and a 597-amino acid mature enzyme of molecular mass 64,173 Da. The consensus sequence, NGTS, of a serine protease substrate primary binding site was identified and a putative ribosome-binding site GGAG occurred 6 bp upstream of the ATG initiation codon.
authors: Department of Biochemistry, Nottingham University Medical School, UK.
authors: P W
authors: PW
authors: Whitby
authors: M
authors: M
authors: Landon
authors: G
authors: G
authors: Coleman
mesh_terms: D000333:Aeromonas / Q000201:enzymology* / Q000235:genetics; D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D001483:Base Sequence; D003001:Cloning, Molecular; D003062:Codon; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D012697:Serine Endopeptidases / Q000737:chemistry / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D003062:Codon; D012697:Serine Endopeptidases; C078810:aspA protein, Aeromonas salmonicida