Application of the environmental SEM in human dentin bleached with hydrogen peroxide in vitro.
The environmental SEM (E-SEM) can be used unfixed biological samples under a low vacuum and wet condition. In this study, the fractured dentin of unfixed human teeth was treated with a 30% hydrogen peroxide solution (H2O2) for the examination of tooth-bleaching prior to the E-SEM and a conventional SEM. The peritubular matrix (PM) always showed a few cracks along the long axis of a dentinal tubule, and the ends of fine fibrils rose to the smoothly changed surface of the intertubular matrix (IM). The E-SEM with non-fixation and the conventional SEM following fixation indicated that the hydrogen peroxide solution easily permeated the PM and dissolved the non-fibrillar substance including the cracks of the PM by the constriction. In the IM, the solution may partially dissolve the organic parts within mineralized fibrils as well as non-fibrillar substance between the fibrils, although these remnants might precipitate again there.
Kodaka
T
T
Department of Oral Anatomy, School of Dentistry, Showa University, Tokyo, Japan.
Toko
T
T
Debari
K
K
Hisamitsu
H
H
Ohmori
A
A
Kawata
S
S
D000293:Adolescent; D000328:Adult; D001641:Bicuspid / Q000648:ultrastructure*; D002648:Child; D003804:Dentin / Q000187:drug effects / Q000648:ultrastructure*; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D008855:Microscopy, Electron, Scanning / Q000379:methods*; D008964:Molar, Third / Q000648:ultrastructure*; D014021:Tissue Preservation / Q000379:methods
D006861:Hydrogen Peroxide