Kinetics of bond cleavages at kallidin release by tissue kallikrein: cleavage of two peptide bonds in a single enzyme-substrate complex?
The kinetics of the release of kallidin, L- and KL-chains from bovine L-kininogen by porcine tissue kallikrein were followed and individual kinetic constants for cleavage of the Met-360 and the Arg-370 bond determined. The results suggest that both these bonds in L-kininogen r are hydrolyzed "simultaneously" without appearance of a free singly-nicked intermediate. Kallidin release in the human analogous system is also compatible with such a mechanism.
Fiedler
F
F
Abtg. Klin. Chem. Klin. Biochem., Chirurg. Klinik Innenstadt d. Universität, München, Germany.
Hinz
H
H
D000818:Animals; D001665:Binding Sites; D002417:Cattle; D006801:Humans; D066298:In Vitro Techniques; D007609:Kallidin / Q000737:chemistry / Q000378:metabolism*; D007610:Kallikreins / Q000378:metabolism*; D007700:Kinetics; D007704:Kininogens / Q000737:chemistry / Q000378:metabolism; D013379:Substrate Specificity; D013552:Swine
D007704:Kininogens; D007609:Kallidin; D007610:Kallikreins