A panel of human chromosome 22-specific sequence tagged sites.
A panel of 29 sequence tagged sites (STSs) covering the long arm of chromosome 22 has been assembled. STS primer pairs were synthesized using available chromosome 22 sequence derived from the GenBank and EMBL DNA sequence databases, as well as published cDNA and genomic sequence, or from previously published and communicated primer pairs. Each STS was optimized for the polymerase chain reaction using a chromosome 22-only hybrid and human genomic DNA. Further STS content analysis on a panel of somatic cell hybrids that incorporated two chromosome 22 translocations resulted in the mapping of the X-box binding protein (XBP), D22S156, and transcobalamin II (TCN2) genes to 22q11-q13.1. The panel of STSs was used for the rapid determination of the STS content and thus the chromosomal DNA content of a new irradiation hybrid.
Collins
J E
JE
Paediatric Research Unit, United Medical School, Guy's Hospital, London, United Kingdom.
Everett
L A
LA
Bentley
D R
DR
Dunham
I
I
D001483:Base Sequence; D002892:Chromosomes, Human, Pair 22; D004247:DNA; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D016324:Sequence Tagged Sites
D004247:DNA