[The protein components of the acid-soluble fraction of normal human blood plasma and in peritonitis patients].
Samples of acid-soluble blood plasma fractions from 10 healthy donors and 12 patients with peritonitis have been fractionated, using the techniques of vertical electrophoresis in polyacrylamide gel and high pressure liquid chromatography. Three basic protein-containing components with the molecular mass 43, 30 and 14 kD have been identified in samples of donor blood plasma. In peritonitis there is an increase in the level of some fractions present in acid-soluble fractions of donor blood plasma and the generation of new protein-containing acid-soluble components. A band corresponding to a component with a molecular mass 64 kD and a wide band in the range of 9-14 kD have been registered. The treatment of donor blood with trypsin generates some components characteristic of acid-soluble blood plasma fraction of patients with peritonitis. The authors believe that increased proteolytic activity is one of the factors responsible for qualitative and quantitative changes in acid-soluble blood plasma fraction in peritonitis.
Semenov
V N
VN
Azizov
Iu M
IuM
Makarshev
I M
IM
Nikolaevskikh
S S
SS
Iastrebova
N E
NE
D000328:Adult; D001782:Blood Donors; D001797:Blood Protein Electrophoresis / Q000379:methods; D001798:Blood Proteins / Q000032:analysis* / Q000302:isolation & purification; D002853:Chromatography, Liquid; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D006801:Humans; D008970:Molecular Weight; D010538:Peritonitis / Q000097:blood*; D012995:Solubility; D013056:Spectrophotometry, Ultraviolet
D001798:Blood Proteins
Belkovye komponenty kislotorastvorimoĭ fraktsii plazmy krovi cheloveka v norme i u bol'nykh peritonitom.