Effects of antiestrogens on adult and neonatal mouse reproductive organs.
Estrogenic potencies of various antiestrogens, including keoxifene (Kx) and trifluoperazine (Tfp), on reproductive tracts of ovariectomized adult mice, and effects of neonatal Kx and Tfp on reproductive organs were studied in C57BL/Tw mice. In adult ovariectomized mice, weight, DNA, and protein of the uterus and vagina were increased by 3 daily injections of 100 micrograms clomiphene, tamoxifen (Tx), and nafoxidine, and of 1 microgram estradiol-17 beta (E), but not by Kx. Antiestrogenic potency of Kx was studied in adult mice given injections of E. Kx significantly suppressed the E-induced increase in weight, DNA, and protein in the uterus and vagina. Tfp (20 micrograms), known as a tranquilizer and an antiestrogen, had no estrogenic effect on either organ. Male and female mice given 5 daily injections of Kx or Tfp from the day of birth were examined at 30, 40, and 60 days of age. Weights of testis, epididymis, and seminal vesicle in neonatally Kx-treated mice were significantly lower than in controls at 30 and 40 days. Spermatozoa were not formed in the seminiferous tubules of Kx-treated mice, although spermatogenesis occurred at 60 days. In neonatally Kx-treated females, weight of the uterus at 60 days and of the vagina at 40 and 60 days was significantly lower than in controls. Corpora lutea were absent from the ovaries of Kx-treated females. In neonatally Tfp-treated mice of both sexes at all ages examined, no differences were found in organ weights or histology, other than lower spermatogenic indices at 40 and 60 days of age.(ABSTRACT TRUNCATED AT 250 WORDS)
Chou
Y C
YC
Department of Biology, Yokohama City University, Japan.
Iguchi
T
T
Bern
H A
HA
D000818:Animals; D000831:Animals, Newborn / Q000502:physiology*; D001835:Body Weight / Q000187:drug effects; D002996:Clomiphene / Q000633:toxicity; D004965:Estrogen Antagonists / Q000633:toxicity*; D005260:Female; D005836:Genitalia, Female / Q000187:drug effects*; D005837:Genitalia, Male / Q000187:drug effects*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009256:Nafoxidine / Q000633:toxicity; D009929:Organ Size / Q000187:drug effects; D010052:Ovariectomy; D010053:Ovary / Q000502:physiology; D010880:Piperidines / Q000633:toxicity; D020849:Raloxifene Hydrochloride; D013629:Tamoxifen / Q000633:toxicity; D014268:Trifluoperazine / Q000633:toxicity; D014599:Uterus / Q000187:drug effects; D014621:Vagina / Q000187:drug effects
D004965:Estrogen Antagonists; D010880:Piperidines; D013629:Tamoxifen; D002996:Clomiphene; D014268:Trifluoperazine; D020849:Raloxifene Hydrochloride; D009256:Nafoxidine