Cholinergic sensitivity of irides from donors with various pathological conditions and lens implants.
In vitro, iris contractions after muscarinic agonists were measured in mg of tension change and the concentration producing 50% of the response was expressed as EC50 mumol/l. Although the average EC50 value of carbachol in the iris sphincter of the donors with diabetes or Parkinson's disease did not change significantly when compared with the control, the maximum contraction of the tissue from the diseased state was increased significantly. Thus, in addition to the well known denervation supersensitivity of the iris-dilator, the iris-sphincter also develops adaptive sensitivity changes. Antimuscarinic drug treatment in some Parkinson's patients interfered with the estimation of supersensitivity in vitro studies. The enhanced response of carbachol at the low temperatures or the relative potency of carbachol and pilocarpine in the tissue obtained from the diseased donors was not significantly different from that of controls. Based on EC50 values, the potency of arecoline on the iris was 1/3 that of carbachol. Significantly lower EC50 values of carbachol were found in irides which were in contact with open loop type anterior chamber lens implants compared with those in contact with the closed loop anterior chamber lens implants. Maximum responses of irides to carbachol were less when the tissue was in contact with open loop lens compared with those in contact with closed loop anterior chamber implants. Irides from many donors having unilateral or bilateral replacement of the artificial lenses responded with EC50 of carbachol which was approximately equal to that of the contralateral eye. The maximum difference between EC50 values of the left and right iris was less than 5 fold.(ABSTRACT TRUNCATED AT 250 WORDS)
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Division of Pharmacology, College of Pharmacy, Ohio State University, Columbus 43210.
Mauger
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D000368:Aged; D000369:Aged, 80 and over; D002217:Carbachol / Q000494:pharmacology; D003920:Diabetes Mellitus / Q000473:pathology*; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D007498:Iris / Q000187:drug effects*; D007910:Lenses, Intraocular; D008297:Male; D008875:Middle Aged; D009116:Muscarine / Q000037:antagonists & inhibitors; D010277:Parasympathomimetics / Q000494:pharmacology*; D010300:Parkinson Disease / Q000473:pathology*; D011976:Receptors, Muscarinic / Q000187:drug effects; D014019:Tissue Donors
D010277:Parasympathomimetics; D011976:Receptors, Muscarinic; D009116:Muscarine; D002217:Carbachol