Direct sequencing and bidirectional allele specific polymerase chain reaction of the bovine beta-casein B variant.
We have used the polymerase chain reaction (PCR) to amplify exon VII of the bovine beta-casein gene. The mutations responsible for the B variant were identified by direct sequencing of the amplification products. A bidirectional allele-specific PCR method (BAS-PCR) has been developed using oligonucleotides overlapping the mutation site at their 3' ends. This new procedure allows a rapid and reliable discrimination between the B and non-B alleles of beta-casein.
Damiani
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Istituto per la Difesa e la Valorizzazione del Germoplasma Animale, CNR, Milano, Italy.
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Leone
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D000483:Alleles; D000818:Animals; D001483:Base Sequence; D002364:Caseins / Q000235:genetics*; D002417:Cattle / Q000235:genetics*; D004247:DNA / Q000235:genetics; D004252:DNA Mutational Analysis; D005069:Evaluation Studies as Topic; D005091:Exons; D005260:Female; D014644:Genetic Variation; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*
D002364:Caseins; D004247:DNA