Mitochondrial dynamics in differentiating fiber cells of the mammalian lens.
The distribution of mitochondria was investigated in living rat and monkey lenses using rhodamine 123 staining and confocal microscopy. In both species, epithelial cells contained abundant small mitochondria scattered throughout the cell volume. Mid-sagittal slices of the living monkey lens revealed that, at the lens equator, mitochondria were only present in fiber cells to a depth of approximately 100 microns. Mitochondria were not present in fiber cells that had already reached the suture line or fibers abutting the central epithelium. Dual-staining with rhodamine 123 and the vital nuclear stain thiazole orange revealed that the loss of nuclei and mitochondria was coincident during fiber cell differentiation.
Bassnett
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Department of Anatomy and Cell Biology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814.
D000818:Animals; D052160:Benzothiazoles; D002454:Cell Differentiation; D004848:Epithelium / Q000648:ultrastructure; D005456:Fluorescent Dyes; D007908:Lens, Crystalline / Q000648:ultrastructure*; D008253:Macaca mulatta; D008856:Microscopy, Fluorescence; D008928:Mitochondria / Q000648:ultrastructure*; D011804:Quinolines; D051381:Rats; D020112:Rhodamine 123; D012235:Rhodamines; D013844:Thiazoles
D052160:Benzothiazoles; D005456:Fluorescent Dyes; D011804:Quinolines; D012235:Rhodamines; D013844:Thiazoles; C051000:thiazole orange; D020112:Rhodamine 123