Fluorimetric DNA assay for cell growth estimation.
A growth assay using the fluorescent dye Hoechst 33258 has been developed which allows sensitive and rapid analysis of the DNA content of a variety of cell types including keratinocytes and mucus-secreting cells, and which requires a minimum of liquid handling. The assay can detect as few as 500 diploid human cells, and is compatible with the simultaneous detection of [3H]thymidine incorporation in the same cultures.
Rao
J
J
Histopathology Unit, Imperial Cancer Research Fund, London, United Kingdom.
Otto
W R
WR
D000818:Animals; D001943:Breast Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D002417:Cattle; D002455:Cell Division / Q000502:physiology; D002478:Cells, Cultured; D004247:DNA / Q000032:analysis*; D004273:DNA, Neoplasm / Q000032:analysis; D005470:Fluorometry / Q000379:methods; D006801:Humans; D015603:Keratinocytes / Q000166:cytology / Q000378:metabolism; D010190:Pancreatic Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D012680:Sensitivity and Specificity; D012997:Solvents; D013936:Thymidine / Q000378:metabolism; D014316:Tritium; D014407:Tumor Cells, Cultured
D004273:DNA, Neoplasm; D012997:Solvents; D014316:Tritium; D004247:DNA; D013936:Thymidine