The effect of sulconazole on the antigenic composition of two dermatophyte species.
Trichophyton rubrum and Trichophyton interdigitale have been grown in liquid culture in the presence of sulconazole. The antigenic activity of detergent extracts of intact organisms was analysed following SDS-PAGE and the probing of Western blots with homologous antisera raised in rabbits and with sera from patients with dermatophyte infections. Differences in protein-band patterns were noted; some bands present in control samples were absent in azole-treated samples and vice versa. These differences were reflected in antigenic band patterns, especially among components of approximate molecular weight of 30-40, 50-60 and 92-100 kDa.
Hearn
V M
VM
Mycological Reference Laboratory, Central Public Health Laboratory, London, UK.
Moore
M K
MK
Latgé
J P
JP
Fracchia
J N
JN
D000818:Animals; D000935:Antifungal Agents / Q000494:pharmacology*; D000946:Antigens, Fungal / Q000032:analysis* / Q000187:drug effects; D003902:Detergents; D005656:Fungal Proteins / Q000032:analysis / Q000187:drug effects; D006023:Glycoproteins / Q000032:analysis / Q000187:drug effects; D006801:Humans; D007093:Imidazoles / Q000494:pharmacology*; D011817:Rabbits; D014249:Trichophyton / Q000187:drug effects* / Q000254:growth & development
D000935:Antifungal Agents; D000946:Antigens, Fungal; D003902:Detergents; D005656:Fungal Proteins; D006023:Glycoproteins; D007093:Imidazoles; C033308:sulconazole