High-performance liquid chromatographic determination of cyanide in human red blood cells by pre-column fluorescence derivatization.
A method for the determination of cyanide in human red cells has been developed. Cyanide was extracted from red cells by adding water and methanol, and then derivatized with 2,3-naphthalene-dialdehyde and taurine to give a fluorescent product, which was determined by reversed-phase high-performance liquid chromatography with fluorescence detection. The recovery of cyanide from red cells was ca. 83%, and the limit of detection was 100 pmol/ml. The mean concentrations of red cell cyanide from ten smokers and from ten non-smokers were 705 and 466 pmol/ml, respectively. The method was also applicable to whole blood.
Sano
A
A
Faculty of Pharmaceutical Sciences, Science University of Tokyo, Japan.
Takimoto
N
N
Takitani
S
S
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003486:Cyanides / Q000097:blood*; D004912:Erythrocytes / Q000737:chemistry*; D005453:Fluorescence; D006801:Humans; D012907:Smoking / Q000097:blood
D003486:Cyanides