Cell-specific expression of aromatase and LH receptor mRNAs in rat ovary.
Current understanding of the endocrine and paracrine regulation of follicular oestrogen synthesis predicts that aromatase cytochrome P450 (P450arom) mRNA is inducible by FSH in granulosa cells. LH receptor mRNA is constitutively expressed in thecal/interstitial cells, and is also thought to be induced in granulosa cells in response to joint stimulation by FSH and oestrogen. This study provides direct evidence that FSH induces the ovarian P450arom gene selectively, perhaps exclusively, in the granulosa cells of Graafian follicles. FSH-induction of LH receptor mRNA occurs simultaneously but is independent of oestrogen synthesis per se.
Whitelaw
P F
PF
Department of Obstetrics and Gynaecology, University of Edinburgh, UK.
Smyth
C D
CD
Howles
C M
CM
Hillier
S G
SG
D000818:Animals; D001141:Aromatase / Q000235:genetics*; D005260:Female; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D015870:Gene Expression / Q000187:drug effects; D007016:Hypophysectomy; D017403:In Situ Hybridization; D010053:Ovary / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D015347:RNA Probes; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D011974:Receptors, LH / Q000235:genetics*
D015347:RNA Probes; D012333:RNA, Messenger; D011974:Receptors, LH; D005640:Follicle Stimulating Hormone; D001141:Aromatase