Circulating antibodies against faecal bacteria assessed by immunomorphometry: combining quantitative immunofluorescence and image analysis.
A new technique to study the prevalence of circulating antibodies directed against different morphological groups ('morphotypes') of bacteria in fresh faeces is presented. The technique combines quantitative indirect immunofluorescence with digital image analysis. Plasma antibody titres and patterns of IgA, IgG and IgM isotype against morphotypes of faecal bacteria were determined in ten healthy individuals.
Apperloo-Renkema
H Z
HZ
Laboratory for Medical Microbiology, State University Groningen, The Netherlands.
Wilkinson
M H
MH
van der Waaij
D
D
D000328:Adult; D000907:Antibodies, Bacterial / Q000097:blood*; D001419:Bacteria / Q000166:cytology / Q000276:immunology*; D005243:Feces / Q000382:microbiology*; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D007070:Immunoglobulin A / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D008297:Male; D008875:Middle Aged; D012680:Sensitivity and Specificity
D000907:Antibodies, Bacterial; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M