Monoclonal antibodies that detect live salmonellae.
Nine immunoglobulin G and nine immunoglobulin M murine monoclonal antibody-producing hybridomas reactive with live Salmonella bacteria were obtained from several fusions of immune spleen cells and Sp2/0 myeloma cells. The antibodies were selected by the magnetic immunoluminescence assay. The monoclonal antibodies were reactive with serogroups A, B, C1, C2, D, E, and K and Salmonella choleraesuis subsp. diarizonae. Each monoclonal antibody proved to be reactive with a distinct serotype. Clinical isolates belonging to these Salmonella serogroups could be detected. Reactivity with non-Salmonella bacteria proved to be minor.
Torensma
R
R
Eijkman-Winkler Laboratory for Medical Microbiology, Utrecht, The Netherlands.
Visser
M J
MJ
Aarsman
C J
CJ
Poppelier
M J
MJ
van Beurden
R
R
Fluit
A C
AC
Verhoef
J
J
D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis; D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D000918:Antibody Specificity; D001431:Bacteriological Techniques; D005069:Evaluation Studies as Topic; D005506:Food Contamination; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007074:Immunoglobulin G / Q000096:biosynthesis; D007075:Immunoglobulin M / Q000096:biosynthesis; D051379:Mice; D012475:Salmonella / Q000145:classification / Q000276:immunology* / Q000302:isolation & purification; D012478:Salmonella Food Poisoning / Q000175:diagnosis; D012703:Serotyping
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D007075:Immunoglobulin M