Conferring new specificities on restriction enzymes: cleavage at any predetermined site by combining adapter oligodeoxynucleotide and class-IIS enzyme.
Podhajska
A J
AJ
Department of Microbiology, University of Gdansk, Poland.
Kim
S C
SC
Szybalski
W
W
D001483:Base Sequence; D004247:DNA / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D004277:DNA, Single-Stranded / Q000378:metabolism*; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism*; D005818:Genetic Engineering / Q000379:methods; D007202:Indicators and Reagents; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000378:metabolism*; D015183:Restriction Mapping; D013379:Substrate Specificity
D004277:DNA, Single-Stranded; D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides; D004247:DNA; D015252:Deoxyribonucleases, Type II Site-Specific