Maleylacetate reductase of Pseudomonas sp. strain B13: dechlorination of chloromaleylacetates, metabolites in the degradation of chloroaromatic compounds.
The maleylacetate reductase of 3-chlorobenzoate-grown cells of Pseudomonas sp. strain B13 has been purified 50-fold. The enzyme converted 2-chloromaleylacetate to 3-oxoadipate with temporary occurrence of maleylacetate; 1 mol of chloride was eliminated during the conversion of 1 mol of 2-chloro- and 2,3-dichloromaleylacetate; 2 mol of NADH were consumed per mol of 2-chloro- and 2,3-dichloromaleylacetate while only 1 mol was necessary to catalyze the conversion of maleylacetate or 2-methylmaleylacetate. The maleylacetate reductase failed to use fumarylacetate as a substrate. The role of the enzyme in the chloroaromatics degradation is discussed.
Kaschabek
S R
SR
Chemische Mikrobiologie der Bergischen Universität-Gesamthochschule Wuppertal, Federal Republic of Germany.
Reineke
W
W
D000272:Adipates / Q000378:metabolism; D001673:Biodegradation, Environmental; D002712:Chlorides / Q000378:metabolism; D002723:Chlorobenzoates / Q000378:metabolism; D007700:Kinetics; D008298:Maleates / Q000378:metabolism*; D009243:NAD / Q000378:metabolism; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000302:isolation & purification / Q000378:metabolism*; D044925:Oxidoreductases Acting on CH-CH Group Donors; D011549:Pseudomonas / Q000201:enzymology*
D000272:Adipates; D002712:Chlorides; D002723:Chlorobenzoates; D008298:Maleates; C036427:3-chlorobenzoic acid; D009243:NAD; C027316:3-oxoadipic acid; C079153:2,3-dichloromaleylacetate; C079154:2-chloromaleylacetate; D010088:Oxidoreductases; D044925:Oxidoreductases Acting on CH-CH Group Donors; C021530:maleylacetate reductase