Single base mutations can be unequivocally and rapidly detected by analysis of DNA heteroduplexes, obtained with deletion-mutant instead of wild-type DNA.
van den Akker
E
E
Department of Oncology, Free University, Amsterdam, The Netherlands.
Braun
J E
JE
Pals
G
G
Lafleur
M V
MV
Retèl
J
J
D004247:DNA / Q000032:analysis* / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D016296:Mutagenesis; D009691:Nucleic Acid Denaturation; D009692:Nucleic Acid Heteroduplexes / Q000032:analysis* / Q000235:genetics; D009695:Nucleic Acid Renaturation; D017384:Sequence Deletion
D009692:Nucleic Acid Heteroduplexes; D004247:DNA