[The effect of normothermic cardioplegic reperfusion with phosphocreatine on the recovery of the cardiac functional indices after total ischemia].
Experiments were conducted on a model of an isolated functioning rat heart to study myocardial protection by normothermic cardioplegic reperfusion (NCR) with phosphocreatine (PC) in 30-minute total ischemia at 37 degrees C. Five series of experiments were performed: (1) cardioplegia (K+ 30 mM/l, Mg2+ 15 nM/l, osmolarity 330 MOSM/l), ischemia, NCR not applied; (2) the same solution was introduced in the preischemic period, ischemia, NCR (K+ 15 mM/l, Mg2+ 15 mM/l, osmolarity 360 MOSM/l); (3) with the same experimental schedule, PC (10 mmol/l) was added to the cardioplegic solution in the preischemic period; (4) in a similar experiment PC was added in the stage of NCR; (5) PC administered in the preischemic stage and in NCR. Restoration of heart functional parameters, rate and ejection of lactate dehydrogenase into the perfusate were compared. The results of the experiment bear evidence that NCR protects the myocardium from reperfusion damage in normothermic ischemia. The optimal cardioprotective effect of PC is produced when it is administered in the preischemic stage. PC added to the solution for NCR has no positive effect on the restoration of heart functional parameters.
Muratov
R M
RM
Kashtélian
L S
LS
Chizhov
A V
AV
D000818:Animals; D002314:Cardioplegic Solutions / Q000494:pharmacology*; D006321:Heart / Q000187:drug effects* / Q000503:physiopathology; D066298:In Vitro Techniques; D008297:Male; D017202:Myocardial Ischemia / Q000503:physiopathology*; D015425:Myocardial Reperfusion / Q000379:methods*; D015428:Myocardial Reperfusion Injury / Q000517:prevention & control; D010725:Phosphocreatine / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013696:Temperature
D002314:Cardioplegic Solutions; D010725:Phosphocreatine
Vliianie normotermicheskoĭ kardioplegicheskoĭ reperfuzii s fosfokreatinom na vosstanovlenie funktsional'nykh pokazateleĭ serdtsa posle total'noĭ ishemii.