Reversibility of fibrinogen fragment D1 binding to human platelets: comparison with native fibrinogen.
To gain further insight into the mechanism responsible for rendering fibrinogen bound to stimulated platelets irreversible to dissociation by EDTA or excess unlabeled fibrinogen, the present study compared the reversibility of platelet interactions with fibrinogen and its plasmic degradation product, fragment D1. Like fibrinogen binding, the binding of fragment D1 became progressively less sensitive to dissociation by EDTA, PGE1, or excess unlabeled fibrinogen. Thus in the presence of EDTA, 70 +/- 19% and 55 +/- 24% (mean +/- S.D., n = 9) of bound fragment D1 failed to dissociate from platelets 60 min after stimulation with 0.15 U/ml thrombin or the combination of 5 microM ADP and 5 microM epinephrine, respectively, compared to 75 +/- 8% and 52 +/- 17% of platelet-bound, intact fibrinogen. In contrast, platelet stimulation with chymotrypsin or Zn+2 failed to support the development of irreversible fragment D1 or fibrinogen binding. Only 8 +/- 6% and 9 +/- 3% of bound fragment D1 remained associated with chymotrypsin- or Zn+2-treated platelets, respectively, compared to 7 +/- 11% and 15 +/- 6% (mean +/- S.D., n = 3) of platelet-associated fibrinogen. These observations suggest that irreversible fragment D1 and fibrinogen binding to platelets occurs by a similar mechanism that requires neither fibrinogen alpha chain 95-97 or 572-574 RGD sequences nor multivalent ligand-receptor interactions.
Peerschke
E I
EI
Department of Pathology, SUNY, Stony Brook 11794-7300.
D000595:Amino Acid Sequence; D001792:Blood Platelets / Q000378:metabolism*; D004492:Edetic Acid; D005338:Fibrin Fibrinogen Degradation Products / Q000378:metabolism*; D005340:Fibrinogen / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data
D005338:Fibrin Fibrinogen Degradation Products; C015214:fibrinogen D fragment; D005340:Fibrinogen; D004492:Edetic Acid