title: The identification of Salmonella enteritidis-infected poultry flocks associated with an outbreak of human salmonellosis.
abstract: In the summer of 1991 a human outbreak of Salmonella enteritidis infection occurred following a barbecue in which about 100 persons were involved. Eggs, supplied by one or more of 10 different layer farms, were the most probable source of the infection. To identify the S. enteritidis-positive flocks, an immunoassay was used to detect salmonella serogroup D-specific antibodies in the yolk of hens eggs. Antibody titres in the eggs from two layer farms, farm A and B, clearly exceeded the titres found in randomly collected eggs. Further investigation on farm A and B yielded high antibody titres in the eggs from flocks A1, A2 and B2, and low titres in the eggs from flock B1. S. enteritidis was isolated from the faecal samples of flocks A1, A2 and B2, whereas no salmonella was detected in the faecal samples of flock B1. The flocks present on both farms originated from the same breeder flock.
authors: Laboratory for Water and Food Microbiology, National Institute of Public Health and Environmental Protection, Bilthoven, The Netherlands.
authors: A W
authors: AW
authors: van de Giessen
authors: J B
authors: JB
authors: Dufrenne
authors: W S
authors: WS
authors: Ritmeester
authors: P A
authors: PA
authors: Berkers
authors: W J
authors: WJ
authors: van Leeuwen
authors: S H
authors: SH
authors: Notermans
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis*; D002645:Chickens / Q000382:microbiology*; D002675:Child, Preschool; D004196:Disease Outbreaks; D004531:Eggs / Q000382:microbiology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D006801:Humans; D009426:Netherlands / Q000453:epidemiology; D012478:Salmonella Food Poisoning / Q000453:epidemiology / Q000382:microbiology*; D012477:Salmonella enteritidis / Q000302:isolation & purification*
chemical_list: D000907:Antibodies, Bacterial