[The effect of naloxone on the activation, inducible by chronic alcoholic intoxication, of the membrane-bound form of enkephalin convertase in the midbrain and hypothalamus of rats].
The activity of membrane-bound and soluble enkephalin convertase was determined with dansyl-Phe-Leu-Arg as substrate in midbrain, including hypothalamus, of Wistar rats, who were given ethanol (20% solution i.g., 9-15 g/kg per day during 4 days) or naloxone (2 mg/kg i.p. twice a day during 4 days) or their combination. It was shown that activation of membrane-bound enzyme, observed after alcohol treatment of rats, didn't develop by combined ethanol-naloxone administration. It's supposed that alcohol-stimulating effect on this enzyme realizes throughout the hyperstimulation of opioid receptors by enkephalins and, possible, by other opioid-active compounds.
Beliaev
N A
NA
Kolesanova
E F
EF
Stanishevskaia
A V
AV
Krzaścik
P
P
Kostowski
W
W
D000437:Alcoholism / Q000201:enzymology*; D000818:Animals; D043423:Carboxypeptidase H; D002268:Carboxypeptidases / Q000187:drug effects / Q000378:metabolism*; D004789:Enzyme Activation / Q000187:drug effects; D007031:Hypothalamus / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D008565:Membrane Proteins / Q000187:drug effects / Q000378:metabolism*; D008636:Mesencephalon / Q000187:drug effects* / Q000201:enzymology; D009270:Naloxone / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D012995:Solubility; D013379:Substrate Specificity / Q000187:drug effects
D008565:Membrane Proteins; D009270:Naloxone; D002268:Carboxypeptidases; D043423:Carboxypeptidase H
Vliianie naloksona na indutsiruemuiu khronicheskoĭ alkogol'noĭ intoksikatsieĭ aktivatsiiu membranno-sviazannoĭ formy énkefalinkonvertazy v srednem mozge i gipotalamuse krys.