A comparison of the different methods available for determining BCG-macrophage interactions in vitro, including a new method of colony counting in broth.
Different methods of determining BCG viability based on colony forming unit (CFU) counting and radio-isotope labelling were comparatively assessed. These included radio-isotope labelling with [3H]uracil, [3H]uridine, [3H]glycerol, and CFU counting, by both agar plate dilution, and microcolony counting in broth. The sensitivity ranges of the different techniques were determined in both macrophage-free and macrophage-treated systems and used to assess the anti-mycobacterial potential of human monocyte-derived macrophages following BCG infection.
Fazal
N
N
Department of Immunology, University of Birmingham, UK.
Bartlett
R
R
Lammas
D A
DA
Kumararatne
D S
DS
D015169:Colony Count, Microbial; D003470:Culture Media; D005990:Glycerol / Q000378:metabolism; D006801:Humans; D007553:Isotope Labeling; D008264:Macrophages / Q000276:immunology*; D009163:Mycobacterium bovis / Q000254:growth & development* / Q000276:immunology; D010587:Phagocytosis; D012680:Sensitivity and Specificity; D014498:Uracil / Q000378:metabolism; D014529:Uridine / Q000378:metabolism
D003470:Culture Media; D014498:Uracil; D005990:Glycerol; D014529:Uridine