Effect of Rb+ on cromakalim-induced relaxation and ion fluxes in guinea pig trachea.
The effects of cromakalim, verapamil and salbutamol have been examined in guinea pig trachealis smooth muscle in both Krebs physiological salt solution and Krebs solution where K+ has been replaced by Rb+. Cromakalim-induced relaxation in the presence of Rb+ was reduced in extent and became transient, whilst the relaxation response to verapamil was enhanced and that to salbutamol unaffected. The transient relaxation occurring in Rb+ was blocked by quinidine and glibenclamide. The presence of extracellular Rb+ also prevented cromakalim-stimulated efflux of both 86Rb+ and 42/43K+. There was, however, no effect on cromakalim-stimulated 86Rb+ uptake. It is proposed that cromakalim is opening two populations of potassium channel in guinea pig tracheal smooth muscle, one of which is susceptible to blockade by Rb+ and one of which is not. The latter channel appears to play the dominant role in cromakalim-stimulated uptake, and is responsible for the transient relaxation response in the presence of rubidium, whilst the former is responsible for the maintained relaxation.
Foster
K A
KA
SmithKline Beecham Pharmaceuticals, Epsom, Surrey, UK.
Arch
J R
JR
Newson
P N
PN
Shaw
D
D
Taylor
S G
SG
D000420:Albuterol / Q000494:pharmacology; D000818:Animals; D001578:Benzopyrans / Q000494:pharmacology*; D001993:Bronchodilator Agents / Q000494:pharmacology*; D019806:Cromakalim; D006168:Guinea Pigs; D066298:In Vitro Techniques; D007545:Isoproterenol / Q000494:pharmacology; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects; D009126:Muscle Relaxation / Q000187:drug effects; D009130:Muscle, Smooth / Q000187:drug effects / Q000378:metabolism*; D011188:Potassium / Q000494:pharmacology; D011758:Pyrroles / Q000494:pharmacology*; D012413:Rubidium / Q000494:pharmacology*; D012414:Rubidium Radioisotopes; D014132:Trachea / Q000187:drug effects / Q000378:metabolism; D014700:Verapamil / Q000494:pharmacology
D001578:Benzopyrans; D001993:Bronchodilator Agents; D011758:Pyrroles; D012414:Rubidium Radioisotopes; D019806:Cromakalim; D014700:Verapamil; D007545:Isoproterenol; D012413:Rubidium; D000420:Albuterol; D011188:Potassium