Ion-pair high-performance liquid chromatography of diaminopimelic acid in hydrolysates of physiological samples.
A method is reported for the determination of diaminopimelic acid (DAPA) in physiological samples. DAPA is derivatized with an o-phthaldialdehyde reagent solution, subjected to reversed-phase high-performance liquid chromatography and detected spectrofluorometrically. The method is a significant advance over previous methods because it uses the ion-pairing agent hexadecyltrimethylammonium bromide (HTMA) to facilitate DAPA measurement. Ion-pairing with HTMA avoids interference with co-eluting derivatives to provide simultaneous, sensitive, reproducible measurement of both DAPA peaks (DD,LL-DAPA and DL-DAPA).
Dugan
M E
ME
Department of Animal Science, University of Alberta, Edmonton, Canada.
Sauer
W C
WC
Lien
K A
KA
Fenton
T W
TW
D000818:Animals; D001419:Bacteria / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003960:Diaminopimelic Acid / Q000032:analysis*; D006868:Hydrolysis; D012417:Rumen / Q000378:metabolism
D003960:Diaminopimelic Acid