Induction and regulation of D-xylose catabolizing enzymes in Fusarium oxysporum.
In Fusarium oxysporum DSM 841, the requirement of oxygen was found to be associated with the induction of key D-xylose catabolizing enzymes. NADPH-linked D-xylose reductase and NAD-linked xylitol dehydrogenase. Both enzymes were detected in cell free extracts of F. oxysporum grown in the medium containing D-xylose, L-arabinose and D-galactose as substrate. D-xylose, L-arabinose and D-galactose were also found to be substrates for NADPH-linked activities. However, unlike xylitol, neither L-arabitol nor galactitol supported the NAD-linked reactions, suggesting the diversion of metabolic pathways of L-arabinose and D-galactose from that of D-xylose following formation of pentitol.
Singh
A
A
Biochemical Engineering Research Centre, Indian Institute of Technology, New Delhi.
Schügerl
K
K
D000449:Aldehyde Reductase / Q000096:biosynthesis* / Q000378:metabolism; D050260:Carbohydrate Metabolism; D050542:D-Xylulose Reductase; D004790:Enzyme Induction; D005670:Fusarium / Q000201:enzymology*; D009243:NAD / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism; D013379:Substrate Specificity; D013401:Sugar Alcohol Dehydrogenases / Q000096:biosynthesis* / Q000378:metabolism; D014994:Xylose / Q000378:metabolism*
D009243:NAD; D009249:NADP; D014994:Xylose; D013401:Sugar Alcohol Dehydrogenases; D000449:Aldehyde Reductase; D050542:D-Xylulose Reductase; D010100:Oxygen