Chemiluminescent detection of alkaline phosphatase in PhastGel.
A chemiluminescent assay has been applied to the detection of alkaline phosphatase on PhastGel containing lysates of preimplantation mouse embryos. The very sensitive detection capabilities reported for the chemiluminescent system led to the investigation of its applicability to the characterization of the alkaline phosphatases in one embryo or less and to compare the sensitivity of two different commercial alkaline phosphatase chemiluminescent assays to a colorimetric assay.
Theodosiou
N G
NG
Genzyme Corporation, Framingham, MA 01701.
Chatot
C L
CL
Ziomek
C A
CA
D000218:Adamantane / Q000031:analogs & derivatives; D000469:Alkaline Phosphatase / Q000032:analysis*; D000818:Animals; D001755:Blastocyst / Q000201:enzymology; D004586:Electrophoresis / Q000379:methods / Q000706:statistics & numerical data; D005069:Evaluation Studies as Topic; D005782:Gels; D008163:Luminescent Measurements; D051379:Mice; D012680:Sensitivity and Specificity
D005782:Gels; C061523:3-(2'-spiroadamantane)-4-methoxy-4-(3''-phosphoryloxy)phenyl-1,2-dioxetane; D000469:Alkaline Phosphatase; D000218:Adamantane