title: Modulation of rat olfactory bulb mitochondrial function by atrial natriuretic peptide.
abstract: Atrial Natriuretic Peptide (ANP) and receptors for ANP are widely distributed in many tissues and cell types in vertebrates. ANP has been shown to be internalized into the cytoplasm in several cell types and thus it raises the possibility that it may act on intracellular receptors. Displacement experiments of [125I]-ANP binding to rat olfactory bulb mitochondrial fraction demonstrated the presence of high affinity (Kd < 10(-9)M) binding sites (Bmax, 112 fmol/mg protein) in this preparation. The addition of ANP (10(-8) M) to this mitochondrial preparation resulted in a 25% increase in TPP+ accumulation, signifying a striking hyperpolarization of the mitochondrial membrane. In contrast ANP did not increase TPP+ uptake to liver mitochondrial preparations. This direct effect of ANP on Olfactory bulb mitochondrial membrane potential may underly the known effects of this hormone on steroidogenesis.
authors: Department of Physiology, Hebrew University, Hadassah Medical School, Jerusalem, Israel.
authors: H
authors: H
authors: Bachar
authors: E
authors: E
authors: Haver
authors: A
authors: A
authors: Ilani
authors: D
authors: D
authors: Lichtstein
mesh_terms: D000818:Animals; D009320:Atrial Natriuretic Factor / Q000378:metabolism / Q000494:pharmacology*; D004594:Electrophysiology; D008928:Mitochondria / Q000187:drug effects* / Q000502:physiology; D008930:Mitochondria, Liver / Q000378:metabolism; D009830:Olfactory Bulb / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D009861:Onium Compounds / Q000493:pharmacokinetics; D009943:Organophosphorus Compounds / Q000493:pharmacokinetics; D051381:Rats
chemical_list: D009861:Onium Compounds; D009943:Organophosphorus Compounds; D009320:Atrial Natriuretic Factor; C013289:tetraphenylphosphonium