[Synthesis cloning and expressions in E coli of human insulin A and B chain genes].
Human insulin A and B chain genes were designed and synthesized by using a rapid and simple method. The synthesized A and B chain genes were cloned separately. The expression (plasmids) pWR 590-HIA and pWR 590-HIB were constructed, and the two plasmids can direct the synthesis of the approximately 590 amino acid-long truncated beta-galactosidases fused to human insulin A or B chains. The fused A or B chain proteins were isolated from the fermented cells and cleaved with BrCN. The resulting mixtures were sulfonated and the sulfonated A and B chains were purified. Human insulin was obtained by using an A and B chain combination method.
Guo
L H
LH
Shanghai Institute of Cell Biology, Academia Sinica.
Wang
E B
EB
Zhang
X Y
XY
Zhu
L H
LH
Yu
Y J
YJ
Wang
Y K
YK
Luo
G X
GX
Zhou
M Y
MY
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics*; D005813:Genes, Synthetic; D006801:Humans; D007328:Insulin / Q000235:genetics*; D008969:Molecular Sequence Data; D010957:Plasmids; D011995:Recombination, Genetic
D007328:Insulin