A new quantitative assay for glycosaminoglycans.
A direct dye-binding technique has been developed to measure total glycosaminoglycans in urine. Fifty or 100 microliters of urine was mixed with a solution of Azure A (10 mg/l) and Azure B (10 mg/l) and the resulting decrease in absorbance at 610 nm was measured. The standard curve with chondroitin sulfate C was linear from 1 to 20 micrograms/assay. The normal value for total urinary glycosaminoglycans in adults was 1.8 +/- 0.6 g/mol creatinine. Other normal values were age-dependent. The assay is inexpensive, simple, precise, sensitive and suitable for screening for the mucopolysaccharidoses in pediatric patients.
Thuy
L P
LP
Department of Pediatrics, University of California, San Diego, La Jolla 92093.
Nyhan
W L
WL
D000293:Adolescent; D000328:Adult; D001399:Azure Stains; D002648:Child; D002675:Child, Preschool; D002809:Chondroitin Sulfates / Q000652:urine; D006025:Glycosaminoglycans / Q000652:urine*; D006801:Humans; D007202:Indicators and Reagents; D007223:Infant; D007231:Infant, Newborn; D009083:Mucopolysaccharidoses / Q000652:urine; D013056:Spectrophotometry, Ultraviolet
D001399:Azure Stains; D006025:Glycosaminoglycans; D007202:Indicators and Reagents; C025819:Azure B; D002809:Chondroitin Sulfates; C025818:Azure A