Adrenalectomy enhances the susceptibility of pancreatic islets to interleukin-1 beta: immunohistochemical study.
To determine the importance of adrenal steroid in the effects of interleukin-1, we investigated changes in the number of islet cells reactive toward antiserum to insulin (anti-Ins) by intraperitoneal administration of recombinant human interleukin-1 beta (IL-1) in intact and adrenalectomized (ADX) rats. IL-1 significantly reduced serum insulin levels in ADX rats only, while it similarly decreased plasma glucose levels. In intact rats, IL-1 did not affect the number of islet cells reactive to anti-Ins, although cytoplasmic immunostaining tended to be reduced by IL-1 treatment. Only adrenalectomy decreased the number of islet cells immunostained by anti-Ins. Furthermore, IL-1 treatment significantly reduced the number of islet cells reactive to anti-Ins in ADX rats. The present study immunohistochemically supported our working hypothesis that the withdrawal of adrenal steroids by adrenalectomy enhances the islet cell sensitivity to exogenous administration of IL-1.
Shimizu
H
H
First Department of Internal Medicine, Gunma University School of Medicine, Japan.
Tanaka
S
S
Mori
M
M
D000311:Adrenal Glands / Q000502:physiology*; D000315:Adrenalectomy; D000704:Analysis of Variance; D000818:Animals; D001786:Blood Glucose / Q000032:analysis / Q000187:drug effects; D007150:Immunohistochemistry; D007274:Injections, Intraperitoneal; D007328:Insulin / Q000097:blood; D007375:Interleukin-1 / Q000494:pharmacology*; D007515:Islets of Langerhans / Q000166:cytology / Q000187:drug effects*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D011994:Recombinant Proteins / Q000494:pharmacology
D001786:Blood Glucose; D007328:Insulin; D007375:Interleukin-1; D011994:Recombinant Proteins