A new individualization marker of semen: deoxyribonuclease I (DNase I) polymorphism.
We describe a method for obtaining specific and reproducible deoxyribonuclease I (DNase I) typing from liquid semen. Isoelectric focusing of the enzymes on polyacrylamide gel (IEF-PAGE, pH 3.5-5) was accomplished using a 0.5-mm thick gel. The separated isozymes were visualized by a new activity staining method, dried agarose film-overlay (DAFO). Pretreatment of semen samples with neuraminidase markedly enhanced the isozyme-band resolution and sensitivity. The method was simple and reliable, with high resolution and sensitivity. The DNase I types in semen samples were correlated with the types found in corresponding blood and urine samples. DNase I typing could therefore provide an additional discriminant characteristic in the forensic examination of semen.
Sawazaki
K
K
Department of Legal Medicine, Fukui Medical School, Japan.
Yasuda
T
T
Nadano
D
D
Tenjo
E
E
Iida
R
R
Takeshita
H
H
Kishi
K
K
D003850:Deoxyribonuclease I / Q000032:analysis*; D005554:Forensic Medicine / Q000379:methods*; D005819:Genetic Markers; D006801:Humans; D007525:Isoelectric Focusing; D008297:Male; D011110:Polymorphism, Genetic; D015203:Reproducibility of Results; D012661:Semen / Q000737:chemistry*
D005819:Genetic Markers; D003850:Deoxyribonuclease I