Effect of freezing of the PCR buffer on the amplification specificity: allelic exclusion and preferential amplification of contaminating molecules.
Hu
C Y
CY
Department of Medical Genetics, University of Uppsala, Sweden.
Allen
M
M
Gyllensten
U
U
D000483:Alleles; D016477:Artifacts; D002021:Buffers; D005615:Freezing; D005802:Genes, MHC Class II; D006683:HLA-DQ Antigens / Q000235:genetics*; D059866:HLA-DQ beta-Chains; D006650:Histocompatibility Testing; D006801:Humans; D015636:Magnesium Chloride; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
D002021:Buffers; D006683:HLA-DQ Antigens; D059866:HLA-DQ beta-Chains; C069051:HLA-DQB1 antigen; D015636:Magnesium Chloride