Time correction for computing Michaelis--Menten kinetics.
A description of time correction applied to the integrated Michaelis equation in view of cancelling a non-correct estimation of the Michaelis constant due to a lack of coincidence between time zero of measurement and time zero of reaction is presented. An estimation of kinetic parameters is made with time correction on the same experimental set of data using a classical least squares iterative procedure.
Rodriguez
F
F
Centre de Recherches de Biochimie et de Génétique Cellulaires, Toulouse, France.
Prats
M
M
D004798:Enzymes / Q000378:metabolism*; D007700:Kinetics; D016018:Least-Squares Analysis; D008838:Microcomputers; D015219:Substrate Cycling; D013997:Time Factors
D004798:Enzymes