HLA-DQ beta 57 in Hispanic patients with insulin-dependent diabetes mellitus.
OBJECTIVE
The purpose of our study was to investigate the distribution of HLA-DQ beta-chain amino acid residue 57 (HLA-DQ beta 57) as a genetic marker of susceptibility for insulin-dependent diabetes mellitus in the Hispanic population.


METHODS
Fifteen patients of Puerto Rican descent with juvenile-onset insulin-dependent diabetes mellitus underwent human leukocyte antigen typing for HLA-DQ beta 57 by polymerase chain reaction amplification of the target genomic DQ sequence followed by hybridization of the polymerase chain reaction product to phosphorus 32-labeled allele-specific oligonucleotide probes. A control group of 44 Hispanic adults without diabetes who were undergoing human leukocyte antigen typing for tissue donation were concurrently typed for comparison.


RESULTS
The Hispanic insulin-dependent diabetes mellitus group showed a significant increase in homozygosity for a non-aspartate amino acid (p = 0.023) over a control group of Hispanic subjects without diabetes. A high rate of heterozygosity for aspartate (53.3%) is found in Hispanic subjects with insulin-dependent diabetes mellitus as well.


CONCLUSIONS
HLA-DQ beta 57 in the Hispanic population has a distribution distinct from HLA-DQ beta 57 in the Caucasian population. A single aspartate is not protective against insulin-dependent diabetes mellitus in Hispanic subjects.
Lee
G
G
Department of Obstetrics and Gynecology, Yale University School of Medicine, New Haven, CT 06510.
Shamma
F N
FN
Diamond
M P
MP
Lee
J T
JT
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000235:genetics; D001483:Base Sequence; D003922:Diabetes Mellitus, Type 1 / Q000208:ethnology / Q000235:genetics / Q000276:immunology*; D005819:Genetic Markers; D020022:Genetic Predisposition to Disease; D006683:HLA-DQ Antigens / Q000032:analysis* / Q000235:genetics; D059866:HLA-DQ beta-Chains; D006579:Heterozygote; D006630:Hispanic or Latino; D006720:Homozygote; D006801:Humans; D015335:Molecular Probes / Q000235:genetics; D008969:Molecular Sequence Data
D000596:Amino Acids; D005819:Genetic Markers; D006683:HLA-DQ Antigens; D059866:HLA-DQ beta-Chains; C057615:HLA-DQbeta antigen; D015335:Molecular Probes