Uptake and metabolism of fluorescent ceramide analogs by rat oligodendrocytes in culture.
We studied the metabolism of sphingolipids by oligodendrocytes derived from rat spinal cord by providing lipid vesicles with either N-lissamine-rhodaminyl-ceramide (LRh-Cer) or N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)-ceramide (NBD-Cer) to the cells cultured in a chemically-defined medium. With both probes the major fluorescent product turned out to be sphingomyelin (SM). Most of LRh-SM was not cell-associated but recovered from the culture medium, probably due to back-exchange to the lipid vesicles. The accumulation of LRh-SM, both in the cells and in the medium, was inhibited in the presence of monensin or brefeldin A, whereas the production of NBD-SM was much less affected by these Golgi perturbing drugs. With LRh-Cer as substrate, LRh-labelled fatty acid (FA), galactosyl- and sulfogalactosyl-ceramides (GalCer and SGalCer) were also formed. NBD-Cer, however, was metabolized to glucosylceramide (GlcCer) and GalCer but not to SGalCer or NBD-FA. These data demonstrate that chemical modifications of ceramide alter its metabolism in oligodendrocytes and that the metabolites of LRh-Cer reflect the glycolipid composition of myelin more closely than those of NBD-Cer.
Giudici
M L
ML
Department of Biomedical Sciences and Biotechnology, University of Brescia, Italy.
Vos
J P
JP
Marchesini
S
S
Van Golde
L M
LM
Lopes-Cardozo
M
M
D000818:Animals; D002478:Cells, Cultured; D002518:Ceramides / Q000378:metabolism*; D002855:Chromatography, Thin Layer; D005260:Female; D005453:Fluorescence; D007700:Kinetics; D009836:Oligodendroglia / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
D002518:Ceramides