A different sort of Mott cell.
NYC is a B lymphoma cell line derived from B/W mice. Upon fusion of NYC cells with a plasmacytoma, which itself produces no immunoglobulin, the resulting NYCH hybridoma cells are Mott cells; i.e., they contain large intracellular vesicles filled with immunoglobulin, the so-called Russell bodies. When NYCH.kappa, a variant of NYCH that had lost the ability to produce heavy chain, was transfected with a heavy-chain construct, this concentration of immunoglobulin in the intracellular vesicles occurred only when the transfected immunoglobulin heavy chain had the same variable region as NYC. Moreover, unlike conventional Mott cells, the hybrid cells secrete immunoglobulin at a normal rate.
Jäck
H M
HM
Department of Microbiology and Immunology, Loyola University Chicago, Maywood, IL 60153.
Beck-Engeser
G
G
Sloan
B
B
Wong
M L
ML
Wabl
M
M
D000818:Animals; D000914:Antibodies, Viral / Q000378:metabolism; D000921:Antibody-Producing Cells / Q000166:cytology*; D001402:B-Lymphocytes / Q000166:cytology*; D005455:Fluorescent Antibody Technique; D006825:Hybridomas / Q000166:cytology*; D007075:Immunoglobulin M / Q000378:metabolism*; D007135:Immunoglobulin Variable Region / Q000378:metabolism; D066298:In Vitro Techniques; D051379:Mice; D015388:Organelles / Q000378:metabolism; D012191:Retroviridae Proteins / Q000276:immunology; D014407:Tumor Cells, Cultured
D000914:Antibodies, Viral; D007075:Immunoglobulin M; D007135:Immunoglobulin Variable Region; D012191:Retroviridae Proteins