Applications of DAPI cytochemistry to neurobiology.
4',6-Diamidino-2-phenylindole hydrochloride (DAPI) is a fluorescent dye with high affinity for DNA. We have employed it as a fluorescent chromatin counterstain on sections immunofluorescent-stained using rhodamine and on tissues enzymatically stained using beta-galactosidase. DAPI also allows easy identification of mitotic figures and can be used to supplement cytochemical studies involving cell division in the nervous system.
Sanna
P P
PP
Department of Neuropharmacology, Scripps Research Institute, La Jolla, California.
Jirikowski
G F
GF
Lewandowski
G A
GA
Bloom
F E
FE
D000818:Animals; D002455:Cell Division / Q000502:physiology; D002467:Cell Nucleus / Q000502:physiology; D002490:Central Nervous System / Q000166:cytology*; D002843:Chromatin / Q000737:chemistry; D004247:DNA / Q000032:analysis; D005455:Fluorescent Antibody Technique; D005456:Fluorescent Dyes; D006651:Histocytochemistry / Q000379:methods; D007211:Indoles; D051379:Mice; D008938:Mitosis / Q000502:physiology; D009446:Neurobiology / Q000379:methods; D051381:Rats; D001616:beta-Galactosidase / Q000032:analysis
D002843:Chromatin; D005456:Fluorescent Dyes; D007211:Indoles; C007293:DAPI; D004247:DNA; D001616:beta-Galactosidase