Gas chromatographic determination of 2- and 3-dechloroethylifosfamide in plasma and urine.
The metabolic oxidation of one of the chloroethyl groups of the antitumour drug ifosfamide leads to the formation of the inactive metabolites 2- and 3-dechloroethylifosfamide together with the neurotoxic metabolite chloroacetaldehyde. A very sensitive capillary gas chromatographic method, requiring only 50 microliters of plasma or urine, has been developed to measure the amounts of the drug and the two inactive metabolites in a single run. Calibration curves were linear (r > 0.999) in the concentration ranges from 50 ng/ml to 100 micrograms/ml in plasma and from 100 ng/ml to 1 mg/ml in urine.
Kaijser
G P
GP
Department of Pharmaceutical Analysis, Faculty of Pharmacy, Utrecht University, Netherlands.
Beijnen
J H
JH
Bult
A
A
Wiese
G
G
de Kraker
J
J
Keizer
H J
HJ
Underberg
W J
WJ
D002849:Chromatography, Gas / Q000295:instrumentation / Q000379:methods*; D003110:Colonic Neoplasms / Q000097:blood / Q000188:drug therapy / Q000652:urine; D003520:Cyclophosphamide / Q000031:analogs & derivatives* / Q000032:analysis / Q000097:blood / Q000652:urine; D006801:Humans; D007069:Ifosfamide / Q000031:analogs & derivatives* / Q000032:analysis / Q000097:blood / Q000378:metabolism / Q000627:therapeutic use / Q000652:urine; D008297:Male; D008875:Middle Aged; D015203:Reproducibility of Results
C064445:dechloroethylcyclophosphamide; D003520:Cyclophosphamide; C038007:dechloroethylifosfamide; D007069:Ifosfamide