Detection of new 7-O-acyl derivatives of diarrhetic shellfish poisoning toxins by liquid chromatography-mass spectrometry.
A novel method for the detection of acylated diarrhetic shellfish poisoning toxins is reported. Direct determination of these compounds is possible using high performance liquid chromatography coupled with ion-spray mass spectrometry. An extract, purified from the digestive glands of toxic mussels (Mytilus edulis) contaminated with okadaic acid, dinophysistoxin-1, and a recently reported analog, dinophysistoxin-2, was also shown to contain small amounts of dinophysistoxin-3, a mixture of 7-O-acyl ester derivatives of dinophysistoxin-1. In addition, acyl ester derivatives of okadaic acid and dinophysistoxin-2 were also detected by direct LC-MS analysis and confirmed by analysis of their hydrolysis products. This is the first report of the detection of other naturally occurring 7-O-acyl esters similar to dinophysistoxin-3.
Marr
J C
JC
Fenwick Laboratories Limited, Halifax, Nova Scotia, Canada.
Hu
T
T
Pleasance
S
S
Quilliam
M A
MA
Wright
J L
JL
D000215:Acylation; D000818:Animals; D049872:Bivalvia / Q000737:chemistry; D002853:Chromatography, Liquid; D002855:Chromatography, Thin Layer; D003967:Diarrhea / Q000139:chemically induced*; D004988:Ethers, Cyclic / Q000633:toxicity; D006868:Hydrolysis; D009682:Magnetic Resonance Spectroscopy; D008387:Marine Toxins / Q000032:analysis* / Q000633:toxicity; D013058:Mass Spectrometry; D019319:Okadaic Acid / Q000031:analogs & derivatives; D011714:Pyrans / Q000633:toxicity; D012758:Shellfish / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet; D014662:Vasoconstrictor Agents / Q000633:toxicity
D004988:Ethers, Cyclic; D008387:Marine Toxins; D011714:Pyrans; D014662:Vasoconstrictor Agents; D019319:Okadaic Acid; C051904:dinophysistoxin 1; C076221:dinophysistoxin 2