title: Immunohistological detection of human cytotoxic/suppressor T cells using antibodies to a CD8 peptide sequence.
abstract: OBJECTIVE
  To evaluate whether cytotoxic/suppressor T cells can be detected in paraffin wax embedded human tissue samples using antibodies to a synthetic CD8 peptide sequence.
  
  
  METHODS
  Polyclonal and monoclonal antibodies were raised against a 13 amino acid peptide sequence from the cytoplasmic portion of the alpha chain of the human CD8 molecule.
  
  
  RESULTS
  These antibodies specifically detected the native form of the CD8 polypeptide when tested by immunoprecipitation with radiolabelled T cells, and gave the expected staining pattern for cytotoxic/suppressor T cells in cryostat sections. Being raised in rabbits, the polyclonal antibodies were also useful for double labelling for CD8 in conjunction with monoclonal antibodies. CD8 positive cells could also be detected in paraffin wax embedded tissues. This was achieved without prior treatment of the sections if the tissue had been fixed in Bouin's fixative. When tissues had been exposed to conventional formalin fixation, preliminary microwave treatment was required.
  
  
  CONCLUSIONS
  These findings provide further evidence that antibodies against leucocyte associated antigens, capable of reacting on paraffin wax embedded tissue, can be produced by immunisation with synthetic peptide sequences.
authors: Department of Haematology, John Radcliffe Hospital, Oxford.
authors: D Y
authors: DY
authors: Mason
authors: J L
authors: JL
authors: Cordell
authors: P
authors: P
authors: Gaulard
authors: A G
authors: AG
authors: Tse
authors: M H
authors: MH
authors: Brown
mesh_terms: D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal; D016827:CD8 Antigens / Q000276:immunology*; D006801:Humans; D007150:Immunohistochemistry; D008969:Molecular Sequence Data; D014066:Palatine Tonsil / Q000378:metabolism; D013154:Spleen / Q000378:metabolism; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D016827:CD8 Antigens