Development of a sensitive enzyme immunoassay for the determination of vinpocetine in human plasma.
An Enzyme immunoassay for the quantitative determination of vinpocetine (CAS-42971-09-5) in human plasma has been developed. The lower limit of quantification is 0.1 ng/ml plasma. The assay shows no cross reactivity with the major metabolite apovincaminic acid. Because of a strong unspecific binding of vinpocetine to plasma proteins an extraction step was necessary. The inter- and intra-assay reproducibility of the test (coefficient of variation) is in a range of 1.1 and 18.3%.
Reck
B
B
Battelle-Institut e.V.a, Frankfurt/Main, Fed. Rep. of Germany.
Dingler
E
E
Lohmann
A
A
D000918:Antibody Specificity; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007124:Immunoenzyme Techniques; D014748:Vinca Alkaloids / Q000097:blood* / Q000276:immunology / Q000493:pharmacokinetics
D014748:Vinca Alkaloids; C032858:apovincaminic acid; C013983:vinpocetine