Lead tetraacetate-thiocarbohydrazide-silver proteinate method for light microscopy of polysaccharides.
Lead tetraacetate-thiocarbohydrazide-silver proteinate reaction sequence for light microscopy of polysaccharides was evaluated on Carnoy's fixed rat liver sections. The results of this evaluation suggest that, on the light microscopic level, the lead tetraacetate-thiocarbohydrazide-silver proteinate method may serve as a practical and histochemically specific alternative to the lead tetraacetate-Schiff reaction for the localization of tissue carbohydrates.
Lo
H K
HK
Department of Orthopaedics and Rehabilitation (R-12), University of Miami School of Medicine, Florida 33101.
Malinin
T I
TI
Malinin
G I
GI
D000818:Animals; D004121:Dimethyl Sulfoxide; D005069:Evaluation Studies as Topic; D016591:Histocytological Preparation Techniques; D006834:Hydrazines; D008027:Light; D008099:Liver / Q000737:chemistry / Q000378:metabolism; D008112:Liver Glycogen / Q000032:analysis / Q000378:metabolism; D008853:Microscopy; D009942:Organometallic Compounds; D010084:Oxidation-Reduction; D011134:Polysaccharides / Q000032:analysis*; D051381:Rats; D012836:Silver Proteins; D000516:alpha-Amylases / Q000378:metabolism
D006834:Hydrazines; D008112:Liver Glycogen; D009942:Organometallic Compounds; D011134:Polysaccharides; D012836:Silver Proteins; C011368:thiocarbohydrazide; C029433:lead tetraacetate; D000516:alpha-Amylases; D004121:Dimethyl Sulfoxide