Construction of a thermotaxis chamber providing spatial or temporal thermal gradients monitored by an infrared video camera system.
A thermotaxis chamber was constructed to quantitatively study thermotaxis in eukaryotic amoeboid cells. The apparatus provided either spatial or temporal temperature gradients in an observation chamber set in an inverted microscope. With an infrared video camera system, spatial thermal gradients were monitored directly and the temperature at the actual location of the cells could be estimated accurately. This enabled a precise determination of the strength of thermal stimuli. With this apparatus, we were able to simultaneously measure temperature and observe cellular behavior directly. This feature permits quantitative studies on stimulus-response relationships. The utility of the apparatus was demonstrated by thermotaxis assay under a spatial thermal gradient in polymorphonuclear leukocytes. Since this apparatus can also provide temporal thermal gradients, it may have several applications in studies of temperature-dependent phenomena in cell biology.
Mizuno
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T
Biological Chemistry Division, National Chemical Laboratory for Industry, Ibaraki, Japan.
Kawasaki
K
K
Miyamoto
H
H
D000656:Amoeba / Q000502:physiology*; D000818:Animals; D002465:Cell Movement; D006801:Humans; D007259:Infrared Rays; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods; D009504:Neutrophils / Q000502:physiology*; D010781:Photography / Q000295:instrumentation / Q000379:methods; D013696:Temperature; D014741:Video Recording / Q000295:instrumentation / Q000379:methods