title: Fluorescence in situ hybridization to determine engraftment status after murine bone marrow transplant.
abstract: The mouse Y-specific DNA sequence pY2 was used as a probe for fluorescence in situ hybridization (FISH) to evaluate murine hematopoietic tissues after sex-mismatched bone marrow transplant (BMT). The pY2 probe was localized to the long arm of the Y chromosome on BM metaphases. Hybridization of pY2 in FISH of interphase cells from BM, spleen, and thymus after BMT was compared with Southern blot analysis; both methods gave comparable results. Only FISH was able to analyze post-BMT peripheral blood (PB) samples successfully, and provides a useful method for following engraftment status in the mouse on an ongoing basis.
authors: Johns Hopkins Oncology Center, Baltimore, MD 21205.
authors: A L
authors: AL
authors: Hawkins
authors: R J
authors: RJ
authors: Jones
authors: B A
authors: BA
authors: Zehnbauer
authors: M S
authors: MS
authors: Zicha
authors: M J
authors: MJ
authors: Collector
authors: S J
authors: SJ
authors: Sharkis
authors: C A
authors: CA
authors: Griffin
mesh_terms: D000818:Animals; D015139:Blotting, Southern; D001854:Bone Marrow Cells; D016026:Bone Marrow Transplantation; D004247:DNA / Q000032:analysis; D015342:DNA Probes; D005260:Female; D005819:Genetic Markers; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D008297:Male; D051379:Mice; D014185:Transplantation, Isogeneic; D014998:Y Chromosome
chemical_list: D015342:DNA Probes; D005819:Genetic Markers; D004247:DNA