title: Long-term expression of a retrovirally introduced beta-galactosidase gene in rodent cells implanted in vivo using biodegradable polymer meshes.
abstract: Grafts of various types of cells have been performed using bioresorbable polymer matrices. These synthetic fibers are degraded by hydrolysis into normal metabolic intermediates and induce a number of events that are conductive to healing and/or repair, the most important of which may be angiogenesis. The use of biodegradable meshes to deliver genetically altered cells was studied. A beta-galactosidase gene was inserted into Long-Evans rat bone marrow stromal (BMS) cells or fibroblasts derived from C57BL/6J mouse embryos using the retroviral vector LNL-SLX beta gal. Expression was monitored using X-gal staining. X-gal+ cells from monolayer cultures were seeded onto either polyglycolic acid (PGA) or polyglactin (PGL) biodegradable meshes and grown to confluence. Two types of grafts were performed: (1) embryonic C57BL/6J mouse fibroblasts (EMF) into either nude mice or adult C57BL/6J mice, and (2) Long-Evans rat BMS into Long-Evans rats. Beta-Galactosidase activity was found for up to 152 days for EMF in nude mice, 123 days for EMF in adult C57BL/6J mice, and 90 days for grafts of syngeneic BMS cells into Long-Evans rats. Noninfected cells grafted using the same methods did not stain with X-gal.
authors: Medical Laboratory Sciences Department, Hunter College School of Health Sciences, New York, New York.
authors: B A
authors: BA
authors: Naughton
authors: Y
authors: Y
authors: Dai
authors: B
authors: B
authors: Sibanda
authors: R
authors: R
authors: Scharfmann
authors: J
authors: J
authors: San Roman
authors: F
authors: F
authors: Zeigler
authors: I M
authors: IM
authors: Verma
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells; D015870:Gene Expression; D005822:Genetic Vectors; D011108:Polymers; D051381:Rats; D012190:Retroviridae / Q000235:genetics; D013630:Tampons, Surgical; D013762:Tetrahydrofolate Dehydrogenase / Q000235:genetics; D013997:Time Factors; D014162:Transfection / Q000379:methods*; D001616:beta-Galactosidase / Q000235:genetics
chemical_list: D011108:Polymers; D013762:Tetrahydrofolate Dehydrogenase; D001616:beta-Galactosidase