title: Simplified template preparation and improved direct sequencing using Taq polymerase.
abstract: A streamlined version of direct dideoxy sequencing is presented that includes template preparation as well as sequencing protocols. The method is used routinely to sequence double-stranded PCR products after minimal purification with one of the primers used in amplification. Either 35S or 32P labeling can be used with equally good results.
authors: Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, Missouri 63110.
authors: A K
authors: AK
authors: Srivastava
authors: V
authors: V
authors: Montanaro
authors: J
authors: J
authors: Kere
mesh_terms: D004259:DNA-Directed DNA Polymerase; D004587:Electrophoresis, Agar Gel; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA / Q000379:methods*; D019914:Taq Polymerase; D013698:Templates, Genetic
chemical_list: D019914:Taq Polymerase; D004259:DNA-Directed DNA Polymerase