[Establishment of EGF, EGF-R and FN-R positive human adenocarcinoma cell line (GAC-1)].
A human adenocarcinoma cell line designated as GAC-1, was established from ascites of the 56-year old male patient with rapidly progressive gastric cancer. The doubling time was about 18.5 hours in vitro, and cell cycle analysis using flow cytometry showed marked increase of S phase (46.1%). Immunohistochemical demonstration of GAC-1 cells revealed positive staining of TGF-alpha, EGF, EGF-R, FN-R, laminin and negative staining of fibronectin. Histogram of them indicated aneuploidy with modal number 57 and they formed tumors in nude mice.
Ohmachi
Y
Y
Department of Surgery, Osaka University Medical School, Japan.
Murata
A
A
Matsuura
N
N
Yasuda
T
T
Yasuda
T
T
Takata
N
N
Oka
Y
Y
Hiraoka
N
N
Nishijima
J
J
Oka
H
H
D000230:Adenocarcinoma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D000818:Animals; D002453:Cell Cycle; D002455:Cell Division; D004815:Epidermal Growth Factor / Q000378:metabolism*; D066246:ErbB Receptors / Q000378:metabolism*; D006801:Humans; D007621:Karyotyping; D007797:Laminin / Q000378:metabolism; D008297:Male; D051379:Mice; D008875:Middle Aged; D017469:Receptors, Fibronectin / Q000378:metabolism*; D013274:Stomach Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D014407:Tumor Cells, Cultured
D007797:Laminin; D017469:Receptors, Fibronectin; D004815:Epidermal Growth Factor; D066246:ErbB Receptors