[Study of the morphofunctional properties of cultured thyrocytes of newborn piglets with the purpose of determining the feasibility of their use for compensation of thyroid gland hypofunction].
The method for cultivation has been developed and morphofunctional characteristics of the newborn piglet thyrocytes studied in order to elucidate the possibility of using these cells for the compensation of the thyroid gland hypofunctioning. The results evidence viability of the cultivated thyrocytes and intactness of their thyroxine-secreting activity within 14 days. Addition of thyrotropin to the medium resulted in the accrement in the population of the active hormone-producing and follicle-producing cells, in reduction of the number of degenerating and pyknotic forms, in stimulation of thyroxine secretion, incorporation of 3H-leucin in thyrocyte protein, and in absorption and organification of 131I by these cells, this being the specific function of this gland, providing the adequate synthesis of thyroid hormones. The resultant thyrocyte culture may be used in clinical transplantology for the treatment of hypofunctional states of the thyroid.
Shostak
I N
IN
Tron'ko
N D
ND
Zurnadzhi
Iu N
IuN
Paster
I P
IP
D000818:Animals; D000831:Animals, Newborn; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured; D004195:Disease Models, Animal; D013552:Swine; D013959:Thyroid Diseases / Q000628:therapy*; D013961:Thyroid Gland / Q000166:cytology* / Q000187:drug effects; D013972:Thyrotropin / Q000494:pharmacology; D013974:Thyroxine / Q000378:metabolism
D013972:Thyrotropin; D013974:Thyroxine
Izuchenie morfofunktsional'nykh svoĭstv kul'tiviruemykh tirotsitov novorozhdennykh porosiat s tsel'iu opredelniia vozmozhnosti ikh primeneniia dlia kompensatsii gipofunktsiĭ shchitovidnoĭ zelezy.