Isolation and structure determination of natural analogues of the mycotoxin ochratoxin A produced by Aspergillus ochraceus.
Three new natural mycotoxins, analogues of ochratoxin A, in which the phenylalanine moiety is replaced by serine, hydroxyproline or lysine, were isolated from cultures of Aspergillus ochraceus by TLC followed by HPLC column chromatography. Their structures were determined after acidic hydrolysis by the characterisation of both their amino acid moieties and of ochratoxin alpha, the chlorinated dihydroisocoumarin moiety of ochratoxin A. These structures were confirmed by mass spectrum analysis.
Hadidane
R
R
Faculté de Médecine Dentaire, Université du Centre, Monastir, Tunisia.
Bacha
H
H
Creppy
E E
EE
Hammami
M
M
Ellouze
F
F
Dirheimer
G
G
D001235:Aspergillus ochraceus / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D013058:Mass Spectrometry; D009793:Ochratoxins / Q000737:chemistry* / Q000302:isolation & purification
D009793:Ochratoxins; C025589:ochratoxin A