Inhibitory effects of hyaluronan on [14C]arachidonic acid release from labeled human synovial fibroblasts.
The effects of hyaluronan (HA) on the release of arachidonic acid (AA) from phospholipids induced by bradykinin in synovial fibroblasts of osteoarthritic patients were examined. HA inhibited [14C]AA release from prelabeled synovial cells stimulated with and without bradykinin 1 hr after incubation with HA and thereafter. The inhibitory effects of HA on [14C]AA release were dependent on the concentration and molecular weight of HA. However, inhibition of [14C]AA release by HA was not merely due to the viscosity of HA. The [14C]AA release induced by calcium-ionophore A23187 was also inhibited by HA with a high molecular weight. In addition, HA did not affect [14C]AA uptake by the cells. Our results suggest that HA with a high molecular weight elicits anti-inflammatory effects, at least in part, by inhibiting AA release in inflamed joints.
Tobetto
K
K
Research Laboratories, Maruho Co., Ltd., Osaka, Japan.
Yasui
T
T
Ando
T
T
Hayaishi
M
M
Motohashi
N
N
Shinogi
M
M
Mori
I
I
D016718:Arachidonic Acid / Q000378:metabolism*; D001920:Bradykinin / Q000494:pharmacology; D000001:Calcimycin / Q000494:pharmacology; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D006820:Hyaluronic Acid / Q000494:pharmacology*; D050356:Lipid Metabolism; D008747:Methylcellulose / Q000494:pharmacology; D008970:Molecular Weight; D010003:Osteoarthritis / Q000188:drug therapy; D013582:Synovial Fluid / Q000166:cytology / Q000378:metabolism*; D014783:Viscosity
D016718:Arachidonic Acid; D000001:Calcimycin; D006820:Hyaluronic Acid; D008747:Methylcellulose; D001920:Bradykinin