title: Assessment of cholinergic influences on a primary humoral immune response.
abstract: Cholinergic ligands can affect some lymphocyte functions, and binding of labelled cholinergic ligands to lymphocytes has been reported. However, the role of endogenous cholinergic stimulation in immunomodulation in vivo is unclear. It has been suggested that suppression of primary humoral immune responses in vivo by administration of organophosphorus compounds is caused by excessive cholinergic stimulation. If this is correct, it would demonstrate cholinergic immunomodulation in vivo and might serve as a useful model for the characterization of this phenomenon. In the present study, the organophosphorus insecticide parathion and its major metabolite, paraoxon, suppressed the primary IgM response to sheep red blood cells (SRBC) in vitro in Mishell-Dutton cultures at concentrations similar to those probably reached in vivo. In contrast, cholinergic agonists did not suppress the in vitro response, but tended to enhance it. However, antagonists also tended to enhance the response and the effects of agonists were not blocked by antagonists. Binding studies with a radiolabelled cholinergic antagonist ([3H-]QNB) did not indicate the presence of specific, saturable cholinergic receptors on lymphocytes. A membrane preparation from brain was used as a positive control, and specific, saturable binding was observed. These results suggest that suppression of primary immune responses in vivo by parathion is mediated at least in part by direct action of parathion and/or its major metabolite, paraoxon, on the immune system. The data provide no evidence that direct interaction of cholinergic ligands with the immune system contributes to parathion-induced immunosuppression. In fact, the absence of expected agonist-antagonist relationships in Mishell-Dutton cultures, the absence of saturable [3H]QNB binding, and puzzling inconsistencies in the literature on this subject cast doubt on the conclusion that lymphocytes express specific cholinergic receptors.
authors: Department of Biological Sciences, Mississippi State University, Mississippi State.
authors: S B
authors: SB
authors: Pruett
authors: Y
authors: Y
authors: Han
authors: A E
authors: AE
authors: Munson
authors: B A
authors: BA
authors: Fuchs
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D000921:Antibody-Producing Cells / Q000187:drug effects / Q000276:immunology*; D004306:Dose-Response Relationship, Immunologic; D004912:Erythrocytes / Q000276:immunology; D005260:Female; D006462:Hemolytic Plaque Technique; D051379:Mice; D008815:Mice, Inbred Strains; D010261:Paraoxon / Q000494:pharmacology; D010277:Parasympathomimetics / Q000037:antagonists & inhibitors / Q000276:immunology*; D010278:Parathion / Q000494:pharmacology; D013154:Spleen / Q000276:immunology
chemical_list: D010277:Parasympathomimetics; D010278:Parathion; D000109:Acetylcholine; D010261:Paraoxon