Specific inhibition of IgE antibody production by an antisense oligodeoxynucleotide oligomer (Oligostick).
We have investigated the ability of an antisense oligonucleotide (ASE-1) to specifically inhibit IgE synthesis by a human myeloma cell line, U266. ASE-1 inhibited IgE production in a concentration-dependent manner, as assessed by isotype-specific ELISA measurement of immunoglobulin in myeloma cell supernatants. Inhibition of IgE production was specific and not due to cytotoxicity since IgG1 and IgM production by human myeloma cell lines ARH-77 and RPMI-1788 respectively, was not significantly affected by up to 20 microM ASE-1 whereas IgE production was inhibited by approximately 70% at this concentration. These results indicate that antisense oligonucleotides represent a potential therapeutic approach to the treatment of IgE-mediated allergic diseases.
Hall
T J
TJ
Department of Immunology, UC and Middlesex School of Medicine (ASH), London.
Brostoff
J
J
D000912:Antibodies, Neoplasm / Q000096:biosynthesis; D002460:Cell Line; D004306:Dose-Response Relationship, Immunologic; D006801:Humans; D006967:Hypersensitivity / Q000188:drug therapy; D007073:Immunoglobulin E / Q000096:biosynthesis*; D007074:Immunoglobulin G / Q000096:biosynthesis; D007075:Immunoglobulin M / Q000096:biosynthesis; D009101:Multiple Myeloma / Q000276:immunology; D016376:Oligonucleotides, Antisense / Q000276:immunology*; D014407:Tumor Cells, Cultured / Q000276:immunology
D000912:Antibodies, Neoplasm; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D016376:Oligonucleotides, Antisense; D007073:Immunoglobulin E