[Examination of the effect of chlorfenvinphos on the activity of aminotransferase in plasma and liver in vitro].
In vitro experiments the effect of chlorfenvinphos on aminotransferases activities in rat plasma and liver homogenate cytoplasmic fraction was studied. As amino groups donors in the transamination reactions with plasma enzymes the next eight amino acids were used: alanine, aspartic acid, cysteine, phenylalanine, leucine, lysine, valine and asparagine, and with the liver cytoplasmatic enzymes--the same above mentioned without asparagine. In all these reactions as an amino groups acceptor alfa-ketoglutaric acid was used. To the incubation mixtures were added: 1 cm3 of the plasma or liver homogenate cytoplasmic fraction; 0.05 cm3 of chlorfenvinphos solution in ethyl acetate (0.17 mg/cm3) or 0.05 cm3 ethyl acetate. Aminotransferase activity was expressed as the amount of glutamate developing during 1 h incubation. Glutamic acid was determined spectrophotometrically after chromatographic separation on filter paper. Both in rat plasma and in liver cytoplasmic fraction all used amino acids as amino groups donors in the transamination reactions were shown. In the reactions with the blood plasma enzymes the most active donors were: alanine, aspartic acid and cysteine and with the participation of liver transaminases: alanine, aspartic acid and phenylalanine. As well in plasma, as in liver the greater activity of AlAT than of AspAT was observed. In the reaction with alanine and aspartate there was formed in the case of plasma 1.51 mumol Glu/cm3 and 1.00 mumol Glu/cm3 respectively and in the case of liver -69.07 mumol Glu/g and 53.26 mumol Glu/g respectively. Results of statistical analysis revealed that small plasma and liver aminotransferases inhibition was caused by the solvent, while the insecticide under test had no influence on the efficiency of transamination.
Maciejewska-Kozak
H
H
Katedry i Zakładu Chemii Toksykologicznej Akademii Medycznej, Lublinie.
Szczepaniak
S
S
Jeleniewicz
K
K
D000818:Animals; D002709:Chlorfenvinphos / Q000494:pharmacology*; D066298:In Vitro Techniques; D008099:Liver / Q000201:enzymology*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D000637:Transaminases / Q000378:metabolism*
D000637:Transaminases; D002709:Chlorfenvinphos
Badania wpływu chlorfenwinfosu na aktywność aminotransferaz osoczowych i watrobowych w warunkach in vitro.