title: Characterization of two novel pyruvylated glycosphingolipids containing 2'-aminoethylphosphoryl(-->6)-galactose from the nervous system of Aplysia kurodai.
abstract: Two novel acidic glycosphingolipids containing pyruvylated galactose were purified from the nervous tissue of Aplysia kurodai by successive Iatrobeads column chromatographies. By component analysis, sugar analysis, permethylation studies, fast atom bombardment-mass spectrometry, and proton magnetic resonance spectrometry, the structures of these acidic glycosphingolipids, named F-9 and FGL-I, were determined to be: [3,4-O-(S-1-carboxyethylidene)]Gal beta 1-->3 GalNAc alpha 1-->3[6'-O-(2-aminoethylphosphonyl)Gal alpha 1-->2] (2-aminoethylphosphoryl 1-->6)Gal beta 1-->4Glc beta 1-->1ceramide and [3,4-O-(S-1-carboxyethylidene)] Gal beta 1-->3GalNAc alpha 1-->3(Fuc alpha 1-->2)(2-aminoethylphosphonyl-->6 Gal beta 1-->4Glc beta 1-->1ceramide, octadeca-4-sphingenine and anteisononadeca-4-sphingenine. Thus, pyruvylated glycosphingolipids containing phosphoethanolamine in addition to or in place of 2-aminoethylphosphonate are present in the nervous system of Aplysia.
authors: Department of Neurochemistry, Faculty of Medicine, Niigata University.
authors: S
authors: S
authors: Araki
authors: S
authors: S
authors: Abe
authors: S
authors: S
authors: Yamada
authors: M
authors: M
authors: Satake
authors: N
authors: N
authors: Fujiwara
authors: K
authors: K
authors: Kon
authors: S
authors: S
authors: Ando
mesh_terms: D020384:Acidic Glycosphingolipids; D000818:Animals; D001048:Aplysia / Q000737:chemistry*; D002240:Carbohydrate Sequence; D003124:Colorimetry; D004983:Ethanolamines / Q000737:chemistry; D005690:Galactose / Q000737:chemistry; D006028:Glycosphingolipids / Q000737:chemistry / Q000302:isolation & purification*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009420:Nervous System / Q000737:chemistry*; D016339:Spectrometry, Mass, Fast Atom Bombardment; D013057:Spectrum Analysis
chemical_list: D020384:Acidic Glycosphingolipids; D004983:Ethanolamines; D006028:Glycosphingolipids; C061817:phosphonoglycosphingolipid FGL-IIb; C079382:F-9 glycosphingolipid; C005448:phosphorylethanolamine; D005690:Galactose