[Method for studying anti-complement reagents: use of freeze-thawed sensitized erythrocytes].
The use of red blood cells coated with complement fractions using low ionic strength saline conditions is part of the evaluation of anti-complement antibodies, specially monoclonal antibodies used for antiglobulin reagents. This work shows that such cells stored in liquid nitrogen allow satisfactory long-term studies except for the anti-C3g antibodies.
Deffune
E
E
Conselho Nacional de Pesquisa Cientifica, Brasilia.
Le Pennec
P Y
PY
Lascaux
J M
JM
Rouger
P
P
D000744:Anemia, Hemolytic, Autoimmune / Q000097:blood / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology; D000918:Antibody Specificity; D001793:Blood Preservation / Q000379:methods; D003176:Complement C3 / Q000276:immunology; D003165:Complement System Proteins / Q000276:immunology*; D003298:Coombs Test / Q000592:standards*; D015925:Cryopreservation / Q000379:methods; D004912:Erythrocytes / Q000276:immunology*; D005189:False Positive Reactions; D006386:Hemagglutination Tests / Q000592:standards; D006801:Humans; D007038:Hypotonic Solutions; D009584:Nitrogen; D012015:Reference Standards; D012680:Sensitivity and Specificity; D012965:Sodium Chloride
D000911:Antibodies, Monoclonal; D003176:Complement C3; D007038:Hypotonic Solutions; D012965:Sodium Chloride; D003165:Complement System Proteins; D009584:Nitrogen
Méthode d'étude des réactifs anti-complément: utilisation d'hématies sensibilisées congelées/décongelées.