title: Neuritogenesis on collagen substrates. Involvement of integrin-like matrix receptors in retinal fibre outgrowth on collagen.
abstract: Extracellular matrix molecules such as laminin, fibronectin and collagen promote neurite outgrowth in vitro. We have investigated the capacity of hydrated gels of collagen types I-III and monomeric collagen types I-VI on plastic surfaces to support neuritogenesis. The attachment and survival of explants from the day 6 chick embryo were studied and neurite outgrowth measured as mean elongation rate and maximal neurite length. Collagen types I and III, both as three-dimensional gels or as native monomers supported neuritogenesis equal to or better than laminin. Collagen type V also supported neurite out-growth although less effectively. Collagen types II, IV and VI, as well as denatured collagens of all types tested, did not support outgrowth. The monoclonal anti-beta 1 integrin antibody (CSAT), as well as rabbit polyclonal antibodies directed to the integrin beta 1-chain, effectively inhibited neurite outgrowth on permissive collagenous substrata, indicating that collagen-binding integrins were involved in the neuritogenesis. These beta 1-integrins were independent of Arg-Gly-Asp (RGD) since neurite formation proceeded in the presence of synthetic RGD-containing peptides. Fluorescence immunohistochemistry revealed the presence of the integrin beta 1-chain on the outgrowing neurites. The results suggest a possible function of collagen and collagen-binding integrins in the development of the visual system.
authors: Department of Developmental Biology, Uppsala University, Sweden.
authors: N G
authors: NG
authors: Carri
authors: K
authors: K
authors: Rubin
authors: D
authors: D
authors: Gullberg
authors: T
authors: T
authors: Ebendal
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002642:Chick Embryo; D003094:Collagen / Q000378:metabolism* / Q000502:physiology; D046508:Culture Techniques; D007202:Indicators and Reagents; D016023:Integrins / Q000276:immunology / Q000378:metabolism*; D016501:Neurites / Q000502:physiology*; D012160:Retina / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*
chemical_list: D000911:Antibodies, Monoclonal; D007202:Indicators and Reagents; D016023:Integrins; D003094:Collagen