Isolation of promoter sequences from Brevibacterium sp. R312.
Promoter sequences recognized by Escherichia coli RNA polymerase were isolated from Brevibacterium sp. R312, a coryneform strain producing nitrile hydratase and amidase. Ten Escherichia coli clones containing promoter sequences were selected for their ability to grow with chloramphenicol concentrations of up to 1500 micrograms/ml. The strength of these promoter sequences was determined. We carried out a preliminary study of the strongest promoter having a chloramphenicol acetyl-transferase/beta-lactamase activities ratio of 18.4.
Duran
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Chaire de Microbiologie Industrielle et de Génétique des Microorganismes, Ecole Nationale Supérieure Agronomique, Montpellier, France.
Chion
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CK
Arnaud
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Galzy
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P
D000581:Amidohydrolases / Q000096:biosynthesis / Q000235:genetics; D001951:Brevibacterium / Q000201:enzymology / Q000235:genetics*; D004269:DNA, Bacterial / Q000032:analysis / Q000302:isolation & purification*; D004926:Escherichia coli / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D005822:Genetic Vectors; D006836:Hydro-Lyases / Q000096:biosynthesis / Q000235:genetics; D011401:Promoter Regions, Genetic; D015183:Restriction Mapping
D004269:DNA, Bacterial; D000581:Amidohydrolases; C019665:amidase; D006836:Hydro-Lyases; C048831:nitrile hydratase