Proteins synthesized and secreted by the guinea-pig conceptus during early pregnancy in relation to corpus luteal maintenance.
Guinea-pig conceptuses obtained on Day 15 of pregnancy were cultured for 24 h in the presence of [3H]leucine. Proteins present in the culture medium were purified by dialysis, desalted, and subjected to analysis by gel filtration chromatography, ion-exchange chromatography, and polyacrylamide gel electrophoresis. The major non-radioactive protein present co-chromatographed with serum albumin. Fresh protein synthesis occurred as indicated by the incorporation of [3H]leucine, and the proteins produced were acidic in nature. Many radioactive proteins in relatively similar amounts were produced, with protein of molecular weights 28.8 and 98.2 kDa (as determined on Sephacryl S-300HR) and of molecular weights 8.4 and 14.7 kDa (as determined on Sephadex G-75SF) being synthesized in marginally greater quantities. Consequently, from the profile of proteins synthesized and secreted, no obvious candidate emerged as the anti-luteolytic factor synthesized and secreted by the guinea-pig conceptus during early pregnancy which inhibits endometrial PGF2 alpha synthesis and maintains corpus luteal function.
Abdi-Dezfuli
F
F
Department of Pharmacology, University of Edinburgh Medical School, UK.
Poyser
N L
NL
D000818:Animals; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D003338:Corpus Luteum / Q000187:drug effects / Q000502:physiology*; D046508:Culture Techniques; D004591:Electrophoresis, Polyacrylamide Gel; D004622:Embryo, Mammalian / Q000378:metabolism*; D005260:Female; D005326:Fetal Proteins / Q000096:biosynthesis* / Q000302:isolation & purification / Q000378:metabolism; D006168:Guinea Pigs; D011247:Pregnancy
D005326:Fetal Proteins