Prolonged survival of Bordetella pertussis in a simple buffer after nasopharyngeal secretion aspiration.
A simple method for recovery of Bordetella pertussis is described using phosphate-buffered saline containing a casein hydrolysate for transporting secretions collected by nasopharyngeal aspirate. Bordetella pertussis was reisolated from 92% of clinical specimens held at 4 degrees C for 1 week and from all specimens held at -20 degrees C. This method will facilitate the centralization of laboratory facilities for the diagnosis of pertussis.
Halperin
S
S
Department of Pediatrics, Dalhousie University, Halifax, N.S., Canada.
Kasina
A
A
Swift
M
M
D001431:Bacteriological Techniques; D001886:Bordetella pertussis / Q000302:isolation & purification*; D002021:Buffers; D003470:Culture Media; D005069:Evaluation Studies as Topic; D006801:Humans; D001239:Inhalation; D009305:Nasopharynx / Q000382:microbiology*; D014917:Whooping Cough / Q000175:diagnosis* / Q000382:microbiology
D002021:Buffers; D003470:Culture Media