Interactions of thermally denatured fibrinogen on polyethylene with plasma proteins and platelets.
During the investigation of fibrin deposition onto hydrophobic polymers in contact with human blood, a model was developed in which fibrinogen was denatured and irreversibly coated onto a polyethylene surface by heating to 70 degrees C for 10 min. The denatured fibrinogen-polyethylene surface is resistant to fluid wall shear rates of up to 550 s-1 and the fibrinogen does not desorb in the presence of plasma proteins. Compared to uncoated polyethylene, little albumin or fibrinogen adsorbs to heat-denatured fibrinogen. Thrombin binds to the denatured fibrinogen-coated polyethylene with low affinity and also acts on the surface-bound denatured fibrinogen and cleaves fibrinopeptide A (FPA) quantitatively. Washed, 51Cr-labeled platelets do not adhere to the thermally denatured fibrinogen at either low or high shear rates compared to surfaces coated with undenatured fibrinogen (p < 0.01). These observations support the role of the D domain of fibrinogen in platelet adhesion because this is the region that is denatured by heating. In contrast, the E domain of fibrinogen is not altered by heating to 70 degrees C and hence remains susceptible to thrombin and/or plasmin cleavage. The characteristics of this surface are such that it can be used to develop fibrin-coated surfaces for use in studies of thrombus formation on artificial surfaces.
Rubens
F
F
Department of Pathology, McMaster University, Hamilton, Ontario, Canada.
Brash
J
J
Weitz
J
J
Kinlough-Rathbone
R
R
D000042:Absorption; D001798:Blood Proteins / Q000737:chemistry*; D002860:Chromium Radioisotopes; D005340:Fibrinogen / Q000737:chemistry*; D005341:Fibrinolysin / Q000737:chemistry; D005344:Fibrinopeptide A / Q000737:chemistry; D006358:Hot Temperature; D006801:Humans; D007457:Iodine Radioisotopes; D010973:Platelet Adhesiveness; D011095:Polyethylenes / Q000737:chemistry*; D011489:Protein Denaturation; D013917:Thrombin / Q000737:chemistry
D001798:Blood Proteins; D002860:Chromium Radioisotopes; D007457:Iodine Radioisotopes; D011095:Polyethylenes; D005344:Fibrinopeptide A; D005340:Fibrinogen; D013917:Thrombin; D005341:Fibrinolysin