Transient expression and histological localization of a gus chimeric gene after direct transfer to mature cowpea embryos.
Transient expression of a chimeric gus reporter gene was demonstrated in cowpea embryonic cells after direct gene transfer. This report shows that seed-derived embryos express the reporter gene at high frequency after passive or electroporation-mediated DNA transfer.
Penza
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Department of Genetics and Cell Biology, Washington State University, Pullman 99164-4234.
Akella
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V
Lurquin
P F
PF
D007887:Fabaceae / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D030821:Plants, Genetically Modified; D010946:Plants, Medicinal; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis*; D014162:Transfection / Q000379:methods*
D011993:Recombinant Fusion Proteins