title: The use of human repetitive DNA to target selectable markers into only the human chromosome of a human-hamster hybrid cell line (AL)
abstract: We used the plasmid BLUR-8 that contains an 800-base pair (bp) sequence of human repetitive Alu DNA in a cotransfection protocol to target the plasmids pSV2neo or EBO-pcD-leu-2 (hygro) into a single site of the sole human chromosome, number 11, of a Chinese hamster-human hybrid cell line (AL). The neo and hygro plasmids confer resistance to the antibiotics G418 and hygromycin, respectively. Of the 33 cotransfected clones with single-site insertions, 1/13 without BLUR-8 and 6/20 with BLUR-8 were only in human chromosome 11. A frequency of insertion of 1/13 is not different than expected by chance (rho = 0.3512). On the other hand, the probability that 6/20 insertions, as seen with BLUR-8, occurred by chance is low (rho = 0.0003). We suggest that the human DNA sequences contained in BLUR-8 targeted insertions into only the human chromosome.
authors: Radiological Health Sciences, Colorado State University, Fort Collins 80523.
authors: C
authors: C
authors: Waldren
authors: M
authors: M
authors: Braaton
authors: D
authors: D
authors: Vannais
authors: B
authors: B
authors: Fouladi
authors: R D
authors: RD
authors: Parker
mesh_terms: D000818:Animals; D015139:Blotting, Southern; D002880:Chromosomes, Human, Pair 11; D006224:Cricetinae; D004560:Electricity; D005818:Genetic Engineering / Q000379:methods*; D006801:Humans; D006822:Hybrid Cells; D010957:Plasmids; D011995:Recombination, Genetic; D012091:Repetitive Sequences, Nucleic Acid; D014162:Transfection