title: Effect of thermodynamic water activity on amino-acid ester synthesis catalyzed by agarose-chymotrypsin in 3-pentanone.
abstract: Chymotrypsin linked to agarose beads by multi-point covalent attachment catalyzes synthesis of Ac-Trp-OEt in 3-pentanone even when the thermodynamic water activity (aw) of the system is reduced to as low as 0.4. If fully hydrated catalyst is added to the reaction mixture before removal of water, product is formed linearly once aw has stabilized. The initial rate is reduced from that if aw is kept close to 1 (0.47 mmol s-1 (kg enzyme)-1), to 50% (aw 0.9), 25% (aw 0.4) and < 1% (aw 0.25). The large drop between aw of 1 and 0.9 probably reflects the effects of water removal on the agarose gel structure. Catalyst partly dried (even only to aw 0.86) before adding to the organic phase is inactive. At reduced aw, the equilibrium (when reached) is shifted in favor of the ester, as expected.
authors: Department of Bioscience and Biotechnology, University of Strathclyde, Glasgow, UK.
authors: R M
authors: RM
authors: Blanco
authors: J L
authors: JL
authors: Rakels
authors: J M
authors: JM
authors: Guisán
authors: P J
authors: PJ
authors: Halling
mesh_terms: D000596:Amino Acids / Q000737:chemistry*; D002918:Chymotrypsin / Q000737:chemistry*; D004952:Esters / Q000138:chemical synthesis*; D010422:Pentanones; D012685:Sepharose; D013816:Thermodynamics; D014867:Water / Q000737:chemistry*
chemical_list: D000596:Amino Acids; D004952:Esters; D010422:Pentanones; D014867:Water; D012685:Sepharose; D002918:Chymotrypsin