Two-dimensional gel electrophoresis analysis of Syrian hamster embryo cells: morphological transformation is not cell type specific.
Studies were conducted to investigate the protein phenotype of normal and morphologically transformed Syrian hamster embryo (SHE) cells. Based upon two-dimensional gel protein phenotype analysis, we conclude that (i) SHE cells are a mixture of multiple cell types including mesenchymal and epithelial cells and (ii) several cell types present in the SHE cell population can be morphologically transformed by a variety of genotoxic and non-genotoxic carcinogens.
Isfort
R J
RJ
Human and Environmental Safety Division, Procter & Gamble Company, Cincinnati, OH 45239-8707.
Kerckaert
G
G
Anderson
N L
NL
LeBoeuf
R A
RA
D000818:Animals; D002273:Carcinogens / Q000633:toxicity*; D002471:Cell Transformation, Neoplastic / Q000139:chemically induced / Q000473:pathology*; D002478:Cells, Cultured; D002999:Clone Cells / Q000187:drug effects; D006224:Cricetinae; D015180:Electrophoresis, Gel, Two-Dimensional; D004848:Epithelium / Q000187:drug effects / Q000473:pathology; D005326:Fetal Proteins / Q000032:analysis*; D008647:Mesocricetus / Q000196:embryology; D008648:Mesoderm / Q000187:drug effects / Q000473:pathology; D009363:Neoplasm Proteins / Q000032:analysis; D010641:Phenotype; D012680:Sensitivity and Specificity
D002273:Carcinogens; D005326:Fetal Proteins; D009363:Neoplasm Proteins