Iron(III) chelating resins II. 3-Hydroxy-4(1H)-pyridinones-sepharose gels.
3-Hydroxy-4(1H)-pyridinones(HP)-Sepharose gels were prepared to study their iron(III) chelating properties. As ligands, derivatives of 3-hydroxy-4(1H)-pyridinone were coupled to CNBr-activated Sepharose gels. HP-Sepharose gels were obtained with HP densities of 23-28 mumol/ml gel and iron(III) chelating capacities of 19-23 mumol/ml gel at pH 6.8. From preliminary experiments, it was found that with the gels 19-27% iron could be removed from milk. In addition, 74% of iron(III) was removed from 100% iron(III) saturated lactoferrin within 24 h at pH 6.8 in the presence of citrate and a Sepharose gel, onto which 1-(2-aminoethyl)-3-hydroxy-2-methyl-4(1H)-pyridinone had been immobilized as a ligand. The properties of the gels make them potentially useful as water-insoluble iron(III) chelating agents.
Feng
M
M
Department of Chemical Technology, University of Twente, Enschede, The Netherlands.
van der Does
L
L
Bantjes
A
A
D000818:Animals; D005782:Gels; D007502:Iron Chelating Agents / Q000737:chemistry*; D007781:Lactoferrin / Q000302:isolation & purification*; D008024:Ligands; D008892:Milk / Q000737:chemistry*; D015394:Molecular Structure; D011728:Pyridones / Q000737:chemistry*; D012117:Resins, Synthetic / Q000737:chemistry*; D012685:Sepharose
D005782:Gels; D007502:Iron Chelating Agents; D008024:Ligands; D011728:Pyridones; D012117:Resins, Synthetic; D012685:Sepharose; D007781:Lactoferrin