title: Immunocytochemical evidence of hypothalamic regulation of adenohypophyseal VIP in the male rat.
abstract: In order to check whether hypothalamic stimulatory or inhibitory factors exert any kind of modulation on the morphology of VIP-immunoreactive cells in the rat hypophysis, the transport of these towards the hypophyseal portal system was blocked by intraventricular administration of colchicine in adult male rats, thereafter performing a morphometric study after characterizing the VIP-immunoreactive adenohypophyseal cells by immunocytochemical techniques. Colchicine administration led to a significant increase (p < 0.01) in the number of reactive cells observed. These cells were characterized by a larger cellular area (p < 0.01), owing to increases in nuclear area (p < 0.05) and cytoplasmic area (p < 0.05). Their morphology became more regular, with a predominance of polygonal and oval cells. The results suggest that the VIP-reactive cells of the rat hypophysis are subject to a hypothalamic inhibitory influence and their activity increasing when the hypothalamic effect is interrupted by intraventricular administration of colchicine.
authors: Departamento de Anatomía e Histología Humanas, Facultad de Medicina, Universidad de Salamanca, Spain.
authors: J
authors: J
authors: Carretero
authors: F
authors: F
authors: Sánchez
authors: M
authors: M
authors: Rubio
authors: M J
authors: MJ
authors: Lorenzo
authors: M
authors: M
authors: Francos
authors: L
authors: L
authors: Cacicedo
authors: F
authors: F
authors: Sánchez-Franco
authors: R
authors: R
authors: Vázquez
mesh_terms: D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D003078:Colchicine / Q000008:administration & dosage; D007031:Hypothalamus / Q000473:pathology / Q000502:physiology*; D007124:Immunoenzyme Techniques; D007276:Injections, Intraventricular; D008297:Male; D010903:Pituitary Gland, Anterior / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014660:Vasoactive Intestinal Peptide / Q000378:metabolism*
chemical_list: D014660:Vasoactive Intestinal Peptide; D003078:Colchicine