[Antiproliferative activity of cimetidine and a copper-cimetidine complex on rat fibroblasts cultured in vitro].
Cimetidine is an active H2-receptor antagonist which, combined with other drugs, has antitumor effect. It has been recently shown that some drugs complexed with copper increase their inhibitory effect on tumor growth and promote cell differentiation. In the present investigation we analysed the modifications of some cell proliferation parameters (mitotic, labelling and apoptotic indices and DNA synthesis) by microfluorometry and autoradiography in 2-day low and high cell density fibroblast cultures in presence of 20 microM cimetidine or copper-cimetidine complex. In low density cultures, a more intense proliferation activity was found while in the high density ones there was accumulation of 4c and 8c polyploid cells. The highest antiproliferative effect was found in low density cultures in presence of copper cimetidine complex, which caused mitotic anomalies and increased chromocenters. Simultaneous evaluation of nuclear DNA content and 3H-T labelling indicated the extension of G1 and G2 phases, followed by a lower cell proliferation and degeneration.
Barni
S
S
Istituto di Fisiologia Generale e Chimica Biologica dell'Università di Sassari.
Sciola
L
L
Pippia
P
P
D000818:Animals; D002455:Cell Division / Q000187:drug effects*; D002478:Cells, Cultured; D002927:Cimetidine / Q000494:pharmacology*; D003300:Copper / Q000494:pharmacology*; D004261:DNA Replication / Q000187:drug effects; D003864:Depression, Chemical; D005347:Fibroblasts / Q000187:drug effects*; D008940:Mitotic Index / Q000187:drug effects; D011123:Polyploidy; D051381:Rats
D003300:Copper; D002927:Cimetidine
Attività antiproliferativa della cimetidina e di un complesso rame-cimetidina in fibroblasti di ratto coltivati in vitro.