Polyreactive IgM antibodies generated from autoimmune mice and selected for histone-binding activity.
A panel of histone-reactive IgM mAbs was obtained from mice belonging to various spontaneously autoimmune strains. Most of these antibodies were polyreactive, i.e. they showed binding to other cationic antigens (poly-L-lysine, lysozyme, cytochrome c) or to cytoskeletal proteins (actin, myosin, vimentin). The variable regions of these antibodies were encoded by V genes and gene segments belonging to various families. Their H chain third hypervariable regions were unusual in that the D segments were read in all three possible reading frames in contrast to most conventional antibodies and other polyreactive antibodies obtained from normal mice.
Novick
K E
KE
Garden State Cancer Center, Newark, NJ 07103.
Fasy
T M
TM
Losman
M J
MJ
Monestier
M
M
D000595:Amino Acid Sequence; D000818:Animals; D000918:Antibody Specificity; D001323:Autoantibodies / Q000235:genetics / Q000276:immunology*; D001327:Autoimmune Diseases / Q000235:genetics / Q000276:immunology*; D001483:Base Sequence; D005260:Female; D015321:Gene Rearrangement; D005803:Genes, Immunoglobulin; D006657:Histones / Q000276:immunology*; D007075:Immunoglobulin M / Q000235:genetics / Q000276:immunology*; D007135:Immunoglobulin Variable Region / Q000235:genetics; D007138:Immunoglobulin delta-Chains / Q000235:genetics; D051379:Mice; D008817:Mice, Mutant Strains / Q000276:immunology*; D008969:Molecular Sequence Data
D001323:Autoantibodies; D006657:Histones; D007075:Immunoglobulin M; D007135:Immunoglobulin Variable Region; D007138:Immunoglobulin delta-Chains