Nucleotide sequence analysis of the class G tetracycline resistance determinant from Vibrio anguillarum.
The nucleotide sequence of the class G tetracycline resistance determinant previously isolated from Vibrio anguillarum has been determined. Two open reading frames of divergent polarity were identified. A resistance gene (tet A) encodes a protein of 393 amino acid residues (deduced molecular mass of 40.9 kDa), and a repressor gene (tet R) encodes a protein consisting of 210 amino acids with a calculated molecular mass of 23.6 kDa. Based on the deduced amino acid sequences, the proteins of tet A(G) and tet R(G) are about 60% homologous with those of RP1/Tn1721 (class A) and pSC101/pBR322 (class C), and about 50% homologous with Tn10 (class B). The relationship of the tet (G) sequence to five known tetracycline resistance determinants (class A to E) is discussed.
Zhao
J
J
Department of Biological Resources, Faculty of Agriculture, Miyazaki University, Japan.
Aoki
T
T
D000595:Amino Acid Sequence; D017920:Antiporters; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D004269:DNA, Bacterial; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D010957:Plasmids; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D013753:Tetracycline Resistance / Q000235:genetics*; D014158:Transcription, Genetic; D014733:Vibrio / Q000187:drug effects / Q000235:genetics*
D017920:Antiporters; D001426:Bacterial Proteins; D004269:DNA, Bacterial; C079051:tetA protein, Bacteria