Simultaneous synthesis of degenerate oligonucleotides of variable length.
Most mutagenic studies have emphasized the exchange of residues but disregarded the variation in length as the other aspect of protein variability. In this report a novel strategy to simultaneously synthesize degenerate mutagenic oligonucleotides of variable length is reported. Synthesis is done on a normal automated DNA synthesizer with modifications only in the program. Product of such synthesis can be used as a mutagenic oligonucleotide for construction of mutant proteins with variable length of inserts.
Jerala
R
R
Dept. of Biochemistry, Jozef Stefan Institute, Ljublijana, Slovenia.
D001483:Base Sequence; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000138:chemical synthesis*; D013777:Tetrazoles
D009841:Oligonucleotides; D013777:Tetrazoles; C045574:1H-tetrazole