Probucol, incorporated into LDL particles in vivo, inhibits generation of lipid peroxides more effectively than endogenous antioxidants alone.
One of the first steps in lipid autoxidation leads to the generation of lipid peroxides (LPO). The time course of LPO generation during Cu++ catalyzed oxidation of LDL before and after treatment with probucol was determined in this study. Before analysis the samples had been stored for about 3 years at -20 degrees C. The results show that in LDL samples without probucol the total antioxidative potential had been depleted during the long-term storage. In contrast, LDL containing probucol showed almost no signs of lipid autoxidation. In addition, the ratio of vitamin E to cholesterol was significantly higher in serum samples containing probucol. We conclude that, in vivo, probucol is incorporated into LDL particles in concentrations high enough to inhibit even early steps of lipid autoxidation.
Baumstark
M W
MW
Medizinische Universitätsklinik, Abteilung Sport- und Leistungsmedizin, Freiburg, Germany.
Arístegui
R
R
Zöller
T
T
Frey
I
I
Berg
A
A
Keul
J
J
D000975:Antioxidants / Q000494:pharmacology*; D002784:Cholesterol / Q000097:blood; D006801:Humans; D006937:Hypercholesterolemia / Q000188:drug therapy; D008054:Lipid Peroxides / Q000037:antagonists & inhibitors*; D008077:Lipoproteins, LDL / Q000008:administration & dosage*; D008297:Male; D011341:Probucol / Q000008:administration & dosage* / Q000494:pharmacology*; D014810:Vitamin E / Q000097:blood
D000975:Antioxidants; D008054:Lipid Peroxides; D008077:Lipoproteins, LDL; D014810:Vitamin E; D002784:Cholesterol; D011341:Probucol