Rapid identification of high affinity peptide ligands using positional scanning synthetic peptide combinatorial libraries.
We describe here a conceptually unique set of individual synthetic peptide combinatorial libraries (SPCLs), termed a positional scanning SPCL (PS-SPCL), that can be used for the rapid (i.e., a single day) identification of peptide sequences that bind with high affinity to antibodies, receptors or other acceptor molecules. The PS-SPCL described here is made up of six individual positional peptide libraries, each one consisting of hexamers with a single position defined and five positions as mixtures. As an example of the utility of such PS-SPCLs, the antigenic determinants recognized by two different monoclonal antibodies were correctly identified upon a single screening.
Pinilla
C
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Torrey Pines Institute for Molecular Studies, San Diego, CA 92121.
Appel
J R
JR
Blanc
P
P
Houghten
R A
RA
D000595:Amino Acid Sequence; D000941:Antigens / Q000235:genetics; D005069:Evaluation Studies as Topic; D015723:Gene Library; D005821:Genetic Techniques; D008024:Ligands; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis / Q000235:genetics* / Q000276:immunology
D000941:Antigens; D008024:Ligands; D010455:Peptides