Susceptibility of trout kidney macrophages to viral hemorrhagic septicemia virus.
Viral hemorrhagic septicemia virus (VHSV) lysed the macrophages from rainbow trout kidney cultures either isolated by plastic adherence or stimulated with purified glycoprotein G from VHSV. The trout macrophages supported the replication of VHSV as tested by cell culture and by sandwich ELISA of the supernatants from infected cultures. VHSV-infected macrophages showed a decrease in both acridine-orange fluorescence and average size. Immunofluorescence studies with flow cytometry showed positive membrane staining with monoclonal antibodies (MAbs) anti-N and anti-G VHSV. These findings open the possibility of using trout macrophages as presenting cells to study the possible existence of helper or cytotoxic epitopes relevant to the protection of trout against VHSV.
Estepa
A
A
INIA-Sanidad Animal, Valdeolmos, Madrid, Spain.
Frías
D
D
Coll
J M
JM
D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay; D005393:Fish Diseases / Q000382:microbiology*; D007668:Kidney / Q000166:cytology; D008264:Macrophages / Q000382:microbiology*; D014337:Trout; D014777:Virus Diseases / Q000662:veterinary; D014780:Viruses / Q000276:immunology* / Q000472:pathogenicity