[DNA of cadaver organs: comparison of two methods of extraction].
Genomic DNA was isolated from tissue specimen by two different methods. The rapid extraction according to Higuchi provided increased product yield of DNA in a better quality than the extraction with phenol/chloroform. Comparing the organ tissues according to quantity and quality of DNA it was found that best yield was recovered from brain tissue followed by lung and liver. DNA stability is directly influenced by postmortal age and age of the bodies.
Kinzinger
G
G
Institut für Rechtsmedizin, Universität Bonn.
Holtz
J
J
D000293:Adolescent; D000328:Adult; D000368:Aged; D001921:Brain / Q000473:pathology; D002648:Child; D002675:Child, Preschool; D004247:DNA / Q000235:genetics / Q000302:isolation & purification*; D016172:DNA Fingerprinting; D005260:Female; D006801:Humans; D007223:Infant; D008099:Liver / Q000473:pathology; D008168:Lung / Q000473:pathology; D008297:Male; D008875:Middle Aged; D008970:Molecular Weight; D011180:Postmortem Changes
D004247:DNA
DNA aus Leichenorganen: Vergleich zweier Extraktionsmethoden.