Improved method for the determination of trimethadione and its demethylated metabolite, dimethadione, in human serum by gas chromatography.
An improved gas chromatographic method, involving the use of a wide-bore capillary column, for the determination of trimethadione and its only demethylated metabolite, dimethadione, in human serum is described. The results indicate that both substances and the internal standard (maleinimide) were well separated with no tailing peak. The detection limit was 10 ng/ml for trimethadione and 50 ng/ml for dimethadione. This improved method is reliable in terms of sensitivity, selectivity and reproducibility for the simultaneous determination of both compounds in human serum.
Tanaka
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E
Institute of Community Medicine, University of Tsukuba, Ibaraki-ken, Japan.
Misawa
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S
D000328:Adult; D002849:Chromatography, Gas / Q000379:methods*; D004114:Dimethadione / Q000097:blood*; D006207:Half-Life; D006801:Humans; D008297:Male; D008875:Middle Aged; D012016:Reference Values; D015203:Reproducibility of Results; D014293:Trimethadione / Q000097:blood*
D004114:Dimethadione; D014293:Trimethadione