[Effects of glucose on proliferation and collagen synthesis in cultured rat mesangial cells].
The influence of glucose concentration on proliferation and collagen production was studied in rat cultured mesangial cells. The cells at passage 6-8 were cultured in 10% FCS/DMEM with different glucose concentration (5, 15 or 25 mM). (3H) thymidine uptake was significantly suppressed in 25 mM glucose condition in the presence or absence of FCS. Immunocytochemistry revealed a dramatic increase in intracellular staining for collagen I, III and IV in 25 mM glucose condition. Analysis of soluble extracellular matrix production using ELISA also showed increased concentration of collagen I, III and IV in the supernatant of mesangial cells grown in 25 mM glucose. The message for collagen I, III and IV increased and decreased rapidly within 24 h after refeeding, making a first peak of message level. After 24 h mRNA for collagen I, III and IV increased again in 25 mM glucose media, whereas the message remained depressed in 5 mM glucose media. These results obtained from in vitro studies suggest that high blood glucose per se may play an important role in increased extracellular matrix in diabetic glomerulosclerosis.
Oshima
S
S
Second Department of Internal Medicine, National Defense Medical College, Saitama, Japan.
Yoshizawa
N
N
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis*; D003928:Diabetic Nephropathies / Q000378:metabolism / Q000473:pathology; D004305:Dose-Response Relationship, Drug; D005920:Glomerular Mesangium / Q000166:cytology / Q000378:metabolism*; D005947:Glucose / Q000494:pharmacology*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013936:Thymidine / Q000378:metabolism
D003094:Collagen; D005947:Glucose; D013936:Thymidine