Changes in fibronectin production in rat liver during cirrhotic evolution due to treatment with CCl4 and steroid hormones: correlation with plasmatic fibronectin.
Previous our studies showed that some steroid hormones, as pure crystalline Progesterone (pPc) and 17-alpha-hydroxyprogesterone capronate (17 alpha HPC) heightened the cirrhogenic action produced in rat liver by carbon tetrachloride. Medroxyprogesterone (MPA), however, did not appear to promote cirrhosis, but increased just steatosis. In the present paper, we have studied the above mentioned steroid hormones for their possible capability of inducing changes in plasma fibronectin concentration. For this purpose, the soluble plasma fibronectin level was measured in female rats 45 days after CCl4-induced cirrhosis, and it was compared with the insoluble fibronectin of liver (detected by immunostaining) and the collagen content in the organ. The results obtained show that, after treatment with CCl4 and MPA, both plasma and liver fibronectin content strongly increases, whereas liver collagen content lowers. However, after treatment with CCl4 alone or in association with the other two steroid hormones, any changes in fibronectin content is not observable, but, on the contrary, is evident a heightened collagen production associated with a cirrhotic change of liver.
Gambella
G R
GR
Istituto di patologia generale, Università di Genova, Italy.
Bellotti
S
S
Cutolo
M
M
Modesti
A
A
Scarpa
S
S
Novelli
A
A
Ravera
G B
GB
D000077713:17 alpha-Hydroxyprogesterone Caproate; D000818:Animals; D002251:Carbon Tetrachloride / Q000008:administration & dosage / Q000633:toxicity*; D003094:Collagen / Q000378:metabolism; D004357:Drug Synergism; D005109:Extracellular Matrix / Q000378:metabolism; D005234:Fatty Liver / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology; D005260:Female; D005353:Fibronectins / Q000096:biosynthesis* / Q000097:blood; D006908:Hydroxyprogesterones / Q000008:administration & dosage / Q000633:toxicity*; D008099:Liver / Q000378:metabolism* / Q000473:pathology; D008106:Liver Cirrhosis, Experimental / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D017258:Medroxyprogesterone Acetate / Q000008:administration & dosage / Q000633:toxicity*; D011374:Progesterone / Q000008:administration & dosage / Q000633:toxicity*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014280:Triglycerides / Q000378:metabolism
D005353:Fibronectins; D006908:Hydroxyprogesterones; D014280:Triglycerides; D000077713:17 alpha-Hydroxyprogesterone Caproate; D011374:Progesterone; D003094:Collagen; D017258:Medroxyprogesterone Acetate; D002251:Carbon Tetrachloride