title: High-performance liquid chromatography-electrochemical detection of 3-methylhistidine in human urine.
abstract: A reversed-phase high-performance liquid chromatographic method is described for the determination of 3-methylhistidine content in human urine using pre-column derivatization with phenylisothiocyanate, isocratic elution with 15 mM sodium acetate-acetonitrile (92:8, v/v) and electrochemical detection. The limit of quantitation was 0.1 pmol. The method has been applied in routine analyses of 3-methylhistidine in both clinical and research work.
authors: Istituto Superiore di Sanità, Rome, Italy.
authors: P
authors: P
authors: Betto
authors: G
authors: G
authors: Ricciarello
authors: S
authors: S
authors: Pichini
authors: L
authors: L
authors: Dello Strologo
authors: G
authors: G
authors: Rizzoni
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004563:Electrochemistry; D006801:Humans; D007223:Infant; D017879:Isothiocyanates; D007676:Kidney Failure, Chronic / Q000652:urine; D008762:Methylhistidines / Q000652:urine*; D013861:Thiocyanates / Q000737:chemistry
chemical_list: D017879:Isothiocyanates; D008762:Methylhistidines; D013861:Thiocyanates; C005441:phenylisothiocyanate; C028118:3-methylhistidine