Chemiluminescence of bronchoalveolar macrophages: effect of adherence to plastic cuvette.
The chemiluminescence of alveolar macrophages was determined in presence of luminol or lucigenin, either before or after the macrophage isolation by adherence to plastic. In presence of luminol, the purified and non-purified macrophages behave almost in the same way, and their chemiluminescence activity is stimulated both by zymosan and phorbol ester. On the other hand, the lucigenin chemiluminescence of non-purified macrophages is considerably higher than that obtained in presence of luminol, but is almost completely insensitive to the zymosan or phorbol ester stimulation. Moreover, the adherence purification considerably inhibits the lucigenin chemiluminescence. These results can be indicative of functionally different macrophage states.
De Sole
P
P
Clinical Chemistry Laboratory, Università Cattolica S. Cuore Rome, Italy.
Frigieri
L
L
Fresu
R
R
Di Mario
G
G
Corvaglia
A G
AG
Petrosino
A
A
Pagliari
G
G
D000166:Acridines; D000328:Adult; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D002448:Cell Adhesion; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D008163:Luminescent Measurements; D008165:Luminol; D008262:Macrophage Activation / Q000187:drug effects; D016676:Macrophages, Alveolar / Q000187:drug effects / Q000502:physiology*; D008297:Male; D008875:Middle Aged; D010969:Plastics; D012507:Sarcoidosis / Q000473:pathology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D015054:Zymosan / Q000494:pharmacology
D000166:Acridines; D010969:Plastics; C033472:10,10'-dimethyl-9,9'-biacridinium; D008165:Luminol; D015054:Zymosan; D013755:Tetradecanoylphorbol Acetate