Detection and separation of some arginine amidases including tissue kallikrein from human seminal plasma.
A plasminogen/plasmin like substance (AHSAA-1), with affinity to lysine column was separated from DEAE-cellulose adsorbed human seminal plasma. Two forms of acidic arginine amidase with different affinities to LBTI (AHSAA-2) and aprotinin columns (AHSAA-3) were separated from the DEAE-cellulose adsorbed preparation and AHSAA-3 was identified as tissue kallikrein. Two basic arginine amidase preparations having affinity to LBTI (BHSAA-1) and aprotinin column were also separated from the CM-cellulose adsorbed human seminal plasma. Three basic arginine amidases with different molecular mass (BHSAA-2 to 4) were separated by Cellulofine GCL-2000 gel filtration from aprotinin adsorbed material and some of their properties were examined.
Matsuda
Y
Y
Department of Biochemistry, Meiji College of Pharmacy (a), Tokyo, Japan.
Kaneko
S
S
Miyazaki
K
K
Kobayashi
T
T
Fujimoto
Y
Y
Akihama
S
S
D000595:Amino Acid Sequence; D002265:Carboxylic Ester Hydrolases / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D002850:Chromatography, Gel; D006801:Humans; D007610:Kallikreins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009842:Oligopeptides / Q000737:chemistry; D012661:Semen / Q000201:enzymology*; D013379:Substrate Specificity
D009842:Oligopeptides; C019724:arginine esterase; D002265:Carboxylic Ester Hydrolases; D007610:Kallikreins