1,25-Dihydroxyvitamin D3 inhibits thromboxane release from activated macrophages.
1,25-Dihydroxyvitamin D3 (1,25-(OH)2D3) in concentrations from 0.1 to 10 nM suppressed immunoreactive thromboxane B2 (iTXB2) release from Propionibacterium acnes (P. acnes)-elicited liver adherent cells stimulated with lipopolysaccharide (LPS, 1 microgram/ml). These suppressive effects of 1,25-(OH)2D3 were also observed in oyster glycogen-elicited peritoneal macrophages. On the contrary, it did not inhibit iTXB2 release from both resident Kupffer cells and peritoneal macrophages stimulated with the same concentration of LPS. Furthermore, 1,24(R)-dihydroxyvitamin D3 (1,24(R)-(OH)2D3), a vitamin D3 analogue, also inhibited iTXB2 release from liver adherent cells, but, another synthesized vitamin D3 analogue, 1 alpha-hydroxyvitamin D3 (1 alpha-OH-D3) tended to decrease iTXB2 release only at higher concentrations. These results suggest that active vitamin D3 analogues inhibit iTXB2 release from activated macrophages.
Horiuchi
H
H
Teijin Institute for Bio-Medical Research, Tokyo, Japan.
Nagata
I
I
Takahashi
K
K
Tsuchimoto
M
M
Komoriya
K
K
D000818:Animals; D002117:Calcitriol / Q000494:pharmacology*; D004100:Dihydroxycholecalciferols / Q000494:pharmacology; D004731:Endotoxins / Q000008:administration & dosage; D007728:Kupffer Cells / Q000187:drug effects; D008070:Lipopolysaccharides; D008099:Liver / Q000187:drug effects; D008262:Macrophage Activation; D008264:Macrophages / Q000378:metabolism*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010529:Peritoneal Cavity; D011425:Propionibacterium acnes; D013929:Thromboxane B2 / Q000037:antagonists & inhibitors* / Q000378:metabolism
D004100:Dihydroxycholecalciferols; D004731:Endotoxins; D008070:Lipopolysaccharides; D013929:Thromboxane B2; C023850:1 alpha,24-dihydroxyvitamin D3; D002117:Calcitriol