Isolation of an Aspergillus terreus mutant impaired in arginine biosynthesis and its complementation with the argB gene from Aspergillus nidulans.
Using filtration enrichment techniques, an Aspergillus terreus arginine auxotrophic strain which contains a mutation that abolishes ornithine transcarbamylase (OTCase) activity has been isolated. This mutant has been genetically transformed with the cloned Aspergillus nidulans OTCase gene. Prototrophic transformants arose at a frequency of about 50 transformants per microgram of plasmid DNA. Southern blot analysis of DNA from the transformants showed that the transforming DNA was ectopically integrated at different locations in the A. terreus genome, often in multiple tandem copies. The transformants were phenotypically stable for several mitotic divisions and retained their capacity to produce extracellular enzymes.
Ventura
L
L
Unidad de Bioingeniería de los Alimentos, Instituto de Agroquímica y Tecnología de los Alimentos (C.S.I.C.), Valencia, Spain.
Ramón
D
D
Pérez-González
J A
JA
D001120:Arginine / Q000096:biosynthesis* / Q000235:genetics*; D001230:Aspergillus / Q000235:genetics* / Q000378:metabolism*; D001233:Aspergillus nidulans / Q000235:genetics* / Q000378:metabolism*; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D009154:Mutation; D009954:Ornithine Carbamoyltransferase / Q000235:genetics; D014170:Transformation, Genetic
D004269:DNA, Bacterial; D001120:Arginine; D009954:Ornithine Carbamoyltransferase