[Carnosine as a stimulator of cytotoxic and phagocytic function of peritoneal macrophages].
Biochemical changes in peritoneal macrophages and their relatedness to the cytostatic and phagocytotic function in C3HA mice injected with a single intraperitoneal dose of 0.45 mM carnosine and 4-methyluracil or stimulated with peptone have been studied. During the first 24 hours after injection both carnosine and 4-methyluracil increase the activity of adenosine deaminase and purine nucleoside phosphorylase, the key enzymes of purine catabolism which is the main source of O2-. radicals in macrophages. In carnosine-stimulated macrophages the activity of membrane 5'-AMP nucleotidase decreases on days 1-3 after injection which points to alleviation of adenosine-induced inhibition as well as to macrophage activation. Carnosine increases the cytostatic and phagocytotic activities of macrophage coupled to O2-. production. The mechanism of the stimulating effect of carnosine on macrophages seems to consist in the dipeptide interaction with specific receptors localized on the plasma membrane of macrophagal cells.
Onufriev
M V
MV
Potanova
G I
GI
Silaeva
S A
SA
Nikolaev
A Ia
AIa
D000243:Adenosine Deaminase / Q000378:metabolism; D000818:Animals; D002336:Carnosine / Q000494:pharmacology*; D005609:Free Radicals; D008163:Luminescent Measurements; D008264:Macrophages / Q000187:drug effects* / Q000276:immunology; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D010100:Oxygen / Q000737:chemistry; D010529:Peritoneal Cavity / Q000166:cytology; D010587:Phagocytosis / Q000187:drug effects*; D011683:Purine-Nucleoside Phosphorylase / Q000378:metabolism
D005609:Free Radicals; D002336:Carnosine; D011683:Purine-Nucleoside Phosphorylase; D000243:Adenosine Deaminase; D010100:Oxygen
Karnozin kak stimuliator tsitostaticheskoĭ i fagotsitarnoĭ funktsiĭ peritoneal'nykh makrofagov.