Determination of oxytetracycline in marine shellfish (Crassostrea gigas, Ruditapes philippinarum and Scrobicularia plana) by high-performance liquid chromatography using solid-phase extraction.
A reversed-phase high-performance liquid chromatographic method was developed to detect oxytetracycline (OTC) in three species of marine shellfish (Crassostrea gigas, Ruditapes philippinarum and Scrobicularia plana). Shellfish tissues were analysed after solid-phase extraction by using a mobile phase containing acetonitrile and 0.02 M orthophosphoric acid solution. The linearity and precision of the method were checked over the concentration range 0.100-1.500 micrograms/g. The limits of detection and determination of OTC were 0.040 and 0.100 microgram/g, respectively. The recovery of OTC from spiked shellfish tissues was 79.8%. The OTC concentration in oyster (Crassostrea gigas) spiked at 0.500 microgram/g and stored at -20 degrees C was stable for 6 weeks. The method was applied to a 21-day experimental study performed with oysters.
Pouliquen
H
H
Service de Pharmacie et Toxicologie, Ecole Nationale Vétérinaire de Nantes, France.
Keita
D
D
Pinault
L
L
D000818:Animals; D002021:Buffers; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000592:standards / Q000706:statistics & numerical data; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008974:Mollusca / Q000737:chemistry; D010118:Oxytetracycline / Q000032:analysis* / Q000493:pharmacokinetics; D012758:Shellfish / Q000032:analysis*
D002021:Buffers; D010118:Oxytetracycline