title: Bioactive ergost-5-ene-3 beta, 7 alpha-diol derivatives from Pseudobersama mossambicensis.
abstract: Bioactivity-directed fractionation of the methyl ethyl ketone extract of Pseudobersama mossambicensis resulted in the isolation of ergosta-5,24(28)-diene-3 beta,7 alpha-diol [1], 24,28-epoxyergost-5-ene-3 beta,7 alpha-diol [2], and ergost-5-ene-3 beta,7 alpha,24,28-tetraol [3]. All three sterols showed selective activity towards DNA repair-deficient yeast mutants. The sterol 1 also showed cytotoxicity towards wild-type P-388 murine leukemia cells. The isolation, structural elucidation, and biological activities of these sterols are reported. The sterol 3 is most probably an artifact formed from 2 during the isolation process.
authors: Department of Chemistry, Virginia Polytechnic Institute and State University, Blacksburg 24061-0212.
authors: A A
authors: AA
authors: Gunatilaka
authors: G
authors: G
authors: Samaranayake
authors: D G
authors: DG
authors: Kingston
authors: G
authors: G
authors: Hoffmann
authors: R K
authors: RK
authors: Johnson
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification* / Q000494:pharmacology; D004260:DNA Repair / Q000187:drug effects; D004354:Drug Screening Assays, Antitumor; D004875:Ergosterol / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000494:pharmacology; D007941:Leukemia P388 / Q000188:drug therapy; D051379:Mice; D010944:Plants / Q000737:chemistry*; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics / Q000378:metabolism
chemical_list: D000972:Antineoplastic Agents, Phytogenic; C079192:ergost-5-ene-3,7-diol; D004875:Ergosterol