[Determining dehydrogenase activity as a method of accelerated indication of the viability of Mycobacterium tuberculosis].
A comparative effectiveness of mycobacterial dehydrogenase activity (DHA) determination was studied with the help of the following stains: methylene blue, malachite green and tetrazole derivative--triphenyl tetrazole chloride (TTC). Best result were obtained with TTC tests which allowed a reliable registration of M. tuberculosis culture viability. On the basis of indication of M. tuberculosis viability by DHA, accelerated techniques were developed to determine mycobacterial drug resistance to the main antituberculous preparations and blood bacteriostatic activity. These techniques are available for practical laboratories, economical and safe in work.
Vishnevskiĭ
B I
BI
Manicheva
O A
OA
D000891:Anti-Infective Agents, Local / Q000494:pharmacology; D000995:Antitubercular Agents / Q000097:blood / Q000494:pharmacology* / Q000627:therapeutic use; D003470:Culture Media; D004353:Drug Evaluation, Preclinical; D006801:Humans; D066298:In Vitro Techniques; D008751:Methylene Blue / Q000494:pharmacology; D008826:Microbial Sensitivity Tests / Q000379:methods*; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000201:enzymology; D010088:Oxidoreductases / Q000378:metabolism*; D012394:Rosaniline Dyes / Q000494:pharmacology; D015610:Serum Bactericidal Test / Q000379:methods*; D013778:Tetrazolium Salts / Q000494:pharmacology; D013997:Time Factors; D014376:Tuberculosis / Q000097:blood / Q000188:drug therapy* / Q000382:microbiology
D000891:Anti-Infective Agents, Local; D000995:Antitubercular Agents; D003470:Culture Media; D012394:Rosaniline Dyes; D013778:Tetrazolium Salts; C005095:malachite green; C009591:triphenyltetrazolium; D010088:Oxidoreductases; D008751:Methylene Blue
Opredelenie degidrogenaznoĭ aktivnosti kak metod uskorennoĭ indikatsii zhiznesposobnosti mikobakteriĭ tuberkuleza