Changes in the distribution of alpha beta and gamma delta T cells in blood and in lymph nodes from fetal and postnatal lambs.
We have examined the distribution of alpha beta and gamma delta T cells, together with B cells, in a range of lymph nodes and blood before and after birth. The proportions of blood-borne alpha beta and gamma delta T cells changed markedly during development with a wave of increasing numbers of blood-borne gamma delta T cells occurring in the fetus during the last month of gestation and early postnatal life. gamma delta T cells constituted 18% of T cells in the blood of fetal lambs one month before birth and 60% of T cells in the blood of lambs one month after birth. There were also changes in the numbers of alpha beta T cells circulating through lymph nodes after birth. The proportion of CD4+ and CD8+ cells in mesenteric nodes increased substantially after birth, whereas that of gamma delta T cells decreased. Although B cells were present in much larger numbers in ileal lymph nodes in both the fetus and lamb, there was a large increase in the concentration of B cells in all lymph nodes in lambs after birth. In addition to the differences in the distribution of lymphocyte subsets circulating in fetal and postnatal lambs, markedly different growth patterns were also observed between lymph nodes before and after birth.
Washington
E A
EA
Department of Veterinary Sciences, University of Melbourne, Parkville, Victoria, Australia.
Kimpton
W G
WG
Cahill
R N
RN
D000375:Aging / Q000276:immunology*; D000818:Animals; D000831:Animals, Newborn / Q000276:immunology*; D001402:B-Lymphocytes; D001773:Blood Cells / Q000276:immunology*; D015704:CD4 Antigens / Q000096:biosynthesis; D016827:CD8 Antigens / Q000096:biosynthesis; D005333:Fetus / Q000276:immunology*; D005434:Flow Cytometry; D007112:Immunity, Maternally-Acquired; D008198:Lymph Nodes / Q000033:anatomy & histology / Q000166:cytology*; D009929:Organ Size; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000096:biosynthesis*; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000096:biosynthesis*; D012756:Sheep / Q000276:immunology*; D013601:T-Lymphocytes / Q000378:metabolism*
D015704:CD4 Antigens; D016827:CD8 Antigens; D016693:Receptors, Antigen, T-Cell, alpha-beta; D016692:Receptors, Antigen, T-Cell, gamma-delta