Does Ca2+ release by acetylcholine enhance the synthesis of inositol 1,4,5-trisphosphate in smooth muscle of the porcine coronary artery?
A possible role was investigated of the Ca2+ released by acetylcholine (ACh) in the ACh-induced synthesis of inositol 1,4,5-trisphosphate (Ins(1,4,5)P3) in smooth muscle of the porcine coronary artery. In Ca(2+)-free solution, 10 microM ACh transiently increased the cellular concentration of Ca2+ ([Ca2+]i) and Ins(1,4,5)P3. Divalent cation ionophores abolished the increase in [Ca2+]i but not the synthesis of Ins(1,4,5)P3 induced by subsequent application of 10 microM ACh in Ca(2+)-free solution, suggesting that the Ca2+ released by Ins(1,4,5)P3 following application of ACh does not act to accelerate the ACh-induced synthesis of Ins(1,4,5)P3 in smooth muscle of the porcine coronary artery.
Kajikuri
J
J
Department of Pharmacology, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Itoh
T
T
Kuriyama
H
H
D000109:Acetylcholine / Q000494:pharmacology*; D000818:Animals; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000032:analysis / Q000378:metabolism*; D003331:Coronary Vessels / Q000737:chemistry / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D015544:Inositol 1,4,5-Trisphosphate / Q000032:analysis / Q000096:biosynthesis*; D015759:Ionomycin / Q000494:pharmacology; D009131:Muscle, Smooth, Vascular / Q000737:chemistry / Q000378:metabolism*; D013552:Swine
D000001:Calcimycin; D015759:Ionomycin; D015544:Inositol 1,4,5-Trisphosphate; D000109:Acetylcholine; D002118:Calcium