Superior prevention of calcium ionophore cataract by E64d.
The purposes of this experiment were: (1), to compare effect of three E64 derivatives, E64, E64c and E64d in preventing nuclear opacity and proteolysis in calcium ionophore-induced cataract and (2), to measure the accumulation of E64 derivatives in the cultured lenses. In vitro E64 and E64c strongly inhibited purified calpain II from porcine heart, while E64d showed weaker inhibition than E64 and E64c. In cultured lenses, all three E64 derivatives reduced nuclear opacity by calcium ionophore A23187 in a concentration-dependent manner, and E64d, the ethyl-ester of E64c, was the most effective. When lenses were cultured in E64d for 2 h, the resulting concentration of E64 derivative in the lens was markedly higher than during culture in E64 or E64c. All three E64 derivatives prevented proteolysis of crystallins seen in A23187 cataract. The stronger effect of E64d against A23187 cataract was likely due to an earlier penetration into the lens, conversion to E64c and inhibition of activated calpain.
Azuma
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Department of Biochemistry and Opthalmology, School of Dentistry Oregon Health Sciences University, Portland 97201.
David
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Shearer
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D000818:Animals; D000001:Calcimycin / Q000037:antagonists & inhibitors; D002118:Calcium / Q000032:analysis; D002154:Calpain / Q000032:analysis / Q000037:antagonists & inhibitors; D002386:Cataract / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*; D003459:Crystallins / Q000378:metabolism; D015853:Cysteine Proteinase Inhibitors / Q000494:pharmacology*; D007908:Lens, Crystalline / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D007930:Leucine / Q000031:analogs & derivatives* / Q000494:pharmacology; D009924:Organ Culture Techniques; D009929:Organ Size; D051381:Rats; D017207:Rats, Sprague-Dawley
D003459:Crystallins; D015853:Cysteine Proteinase Inhibitors; D000001:Calcimycin; D002154:Calpain; D007930:Leucine; C024974:E 64; D002118:Calcium