title: Histochemical and contractile responses of rat medial gastrocnemius to 2 weeks of complete disuse.
abstract: We studied the histochemical and in situ contractile changes in a rat ankle extensor, medial gastrocnemius, in which activation of muscle fibres by motoneurones was blocked for 14 days, using the sodium channel blocker tetrodotoxin applied to the sciatic nerve. Muscles were atrophied and showed slower twitch responses, greater fusion at subtetanic frequencies of stimulation, and higher twitch/tetanic ratios. Tetanic force/mm2 of fibre area and fatiguability were unchanged. Type II fibres were more atrophied and showed greater decreases in mitochondrial succinate dehydrogenase activity than type I fibres. The contractile changes resulting from complete disuse do not occur in models in which weight-bearing alone has been removed (space flight, hindlimb suspension), suggesting that the residual motoneurone activity reported in models of weightlessness is sufficient to prevent these responses. Similarly, the finding of a greater type II fibre susceptibility to complete disuse, which differs from the pattern seen in models of weightlessness, suggest that this residual motoneurone activity in the latter influences atrophic responses in a manner that is variable among motor unit types, to produce the reported preferential type I atrophy characteristic of removal of weight-bearing.
authors: Sciences de l'activité physique, Université de Montréal (Qué.), Canada.
authors: P F
authors: PF
authors: Gardiner
authors: M
authors: M
authors: Favron
authors: P
authors: P
authors: Corriveau
mesh_terms: D000818:Animals; D001284:Atrophy / Q000473:pathology; D004594:Electrophysiology; D005260:Female; D006614:Hindlimb / Q000294:innervation / Q000502:physiology; D006651:Histocytochemistry; D008931:Mitochondria, Muscle / Q000201:enzymology; D009046:Motor Neurons / Q000502:physiology; D009119:Muscle Contraction / Q000502:physiology; D009132:Muscles / Q000166:cytology / Q000473:pathology / Q000502:physiology*; D009210:Myofibrils / Q000201:enzymology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013385:Succinate Dehydrogenase / Q000378:metabolism; D013779:Tetrodotoxin / Q000494:pharmacology; D014893:Weightlessness
chemical_list: D013779:Tetrodotoxin; D013385:Succinate Dehydrogenase