Changes of urea-soluble and intrinsic membrane proteins in rat lenses during the formation of galactose cataract.
Urea-soluble and intrinsic membrane proteins from normal and galactose cataractous rat lenses were analyzed by SDS-PAGE. During cataract formation, MP22 increased whereas MP26 decreased almost to nil and MP24 emerged. However, the relative amount of MP18 remained essentially unchanged. These results suggested a conversion of MP26 to MP22 during cataract formation. We also observed the changes in the relative abundance of the polypeptides of the urea-soluble fraction with cataractogenesis.
Zhao
H R
HR
Department of Biochemistry, Xuzhou Medical College, Jiangsu, ROC.
Ren
X H
XH
D000818:Animals; D002386:Cataract / Q000139:chemically induced / Q000378:metabolism*; D003459:Crystallins / Q000378:metabolism*; D004195:Disease Models, Animal; D004591:Electrophoresis, Polyacrylamide Gel; D005690:Galactose; D007908:Lens, Crystalline / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism*; D008970:Molecular Weight; D051381:Rats; D017207:Rats, Sprague-Dawley; D012995:Solubility; D014508:Urea / Q000378:metabolism*
D003459:Crystallins; D008565:Membrane Proteins; D014508:Urea; D005690:Galactose