Are thrombocyte membranes altered in Alzheimer's disease? A morphometric and biochemical study.
Morphometric analysis of thrombocytes from patients with Alzheimer's disease, from patients with multi-infarct dementia, and from young and age-matched healthy control donors, did not reveal any Alzheimer-related increase in internal membranes. Biochemical analysis showed a reduced cholesterol content of thrombocyte membrane preparations from Alzheimer patients relative to age-matched controls, but not relative to multi-infarct dementia patients. Overall distribution of protein kinase C activity (PKC) between cytosol and membrane, in resting as well as in activated thrombocytes from Alzheimer patients, was similar to that in the control groups. However, both Alzheimer and multi-infarct dementia patients had lower cytosolic levels of basal kinase and PKC activities than age-matched controls, while only Alzheimer patients had lower cytoskeletal PKC activity than controls.
Bosman
G J
GJ
Department of Biochemistry, Faculty of Medicine, University of Nijmegen, The Netherlands.
Schuurmans Stekhoven
J H
JH
Melenhorst
J J
JJ
Van Zuylen
A J
AJ
Bartholomeus
I G
IG
Van Kalmthout
P J
PJ
De Grip
W J
WJ
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000378:metabolism; D000544:Alzheimer Disease / Q000378:metabolism* / Q000473:pathology; D001792:Blood Platelets / Q000378:metabolism* / Q000648:ultrastructure; D002462:Cell Membrane / Q000378:metabolism / Q000648:ultrastructure; D006801:Humans; D008563:Membrane Lipids / Q000378:metabolism; D011493:Protein Kinase C / Q000378:metabolism
D008563:Membrane Lipids; D011493:Protein Kinase C