Human sperm surface mapping with lectins.
Ten fluorescein isothiocyanate (FITC)-linked lectins [Bauhimia purpurea, Concanavalin A, Dolichos biflorus (DBA), Griffonia simplicifolia I, Griffonia simplicifolia II, Maclura pomifera, Arachis hypogea (PNA), Glycine max, Ulex europaeus (UEA) and Triticum vulgaris agglutinin] have been used to study their binding features on the human ejaculate spermatozoa. Qualitative changes in the labeling pattern have been observed in unfixed and acetone-treated spermatozoa. Furthermore, ultrastructural localization of some of the colloidal gold-linked lectins, namely PNA, UEA and DBA, has been attempted to delineate the binding domains of the specific sugars on the sperm surface. It needs to be emphasized that flow-cytometric methods employed in our study, which provide quantitative slant to qualitative data, should be utilized to evaluate the functional status of the spermatozoa.
Bains
H K
HK
Department of Biophysics, Panjab University, India.
Sehgal
S
S
Bawa
S R
SR
D002462:Cell Membrane / Q000648:ultrastructure*; D006801:Humans; D037102:Lectins; D008297:Male; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D013094:Spermatozoa / Q000166:cytology*
D037102:Lectins