Vanadate and insulin stimulate gene 33 expression.
Vanadate and insulin stimulated gene 33 expression in rat H4 hepatoma cells. Vanadate (10(-3) M) maximally increased gene 33 transcription 4-fold at 15 min. The maximal insulin induction (3-4-fold) was observed using 5 x 10(-9) M insulin for 30 min. Both vanadate and insulin increased cytoplasmic mRNA levels in a dose-dependent manner, with maximal effects observed by 60 min. These effects on gene 33 mRNA accumulation were greater than those on transcription. Vanadate (10(-3) M) maximally stimulated gene 33 mRNA levels 10-12-fold. The vanadate and insulin effects were not synergistic. Thus, high concentrations of vanadate regulate gene 33 expression in an insulin-like manner and, like insulin, vanadate probably controls transcriptional as well as post-transcriptional events.
Weinstock
R S
RS
Department of Veterans Affairs Medical Center, Syracuse, NY.
Messina
J L
JL
D000818:Animals; D003593:Cytoplasm / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D015870:Gene Expression / Q000187:drug effects; D007328:Insulin / Q000494:pharmacology*; D007700:Kinetics; D008114:Liver Neoplasms, Experimental; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D014158:Transcription, Genetic / Q000187:drug effects*; D014407:Tumor Cells, Cultured; D014638:Vanadates / Q000494:pharmacology*
D007328:Insulin; D012333:RNA, Messenger; D014638:Vanadates