Determination of free amino acid enantiomers in rat brain and serum by high-performance liquid chromatography after derivatization with N-tert.-butyloxycarbonyl-L-cysteine and o-phthaldialdehyde.
The concurrent determination of free amino acid enantiomers and non-chiral amino acids in rat brain and serum was accomplished by high-performance liquid chromatography with fluorimetric detection after derivatization with N-tert.-butyloxycarbonyl-L-cysteine and o-phthaldialdehyde. The method revealed the presence of a large amount of free D-serine (0.22 mumol/g of tissue; D/D + L ratio = 0.25) in the brain whereas D-aspartate and D-alanine were established to be at trace levels. These results further support the presence of D-serine in adult brain tissues as demonstrated by recent work using gas chromatography.
Hashimoto
A
A
Division of Mental Disorder Research, National Institute of Neuroscience, NCNP, Tokyo, Japan.
Nishikawa
T
T
Oka
T
T
Takahashi
K
K
Hayashi
T
T
D000596:Amino Acids / Q000032:analysis* / Q000097:blood*; D000818:Animals; D001923:Brain Chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003545:Cysteine / Q000031:analogs & derivatives*; D005470:Fluorometry; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013237:Stereoisomerism; D009764:o-Phthalaldehyde
D000596:Amino Acids; C079451:N-tert-butyloxycarbonylcysteine; D009764:o-Phthalaldehyde; D003545:Cysteine