Pyrimidine base and ribonucleoside catabolic enzyme activities of the Pseudomonas diminuta group.
Pyrimidine base and ribonucleoside catabolic enzyme activities of the two type strains of the Pseudomonas diminuta group were investigated for taxonomic classification purposes. The presence of the pyrimidine salvage enzyme nucleoside hydrolase was indicated in both type strains following thin-layer chromatographic analysis. The presence of the hydrolase was also confirmed by enzyme assay. In addition, the activities of the pyrimidine salvage enzymes dihydropyrimidine dehydrogenase and dihydropyrimidinase were measurable in cell-free extracts of both P. diminuta and P. vesicularis. An absence of cytosine deaminase activity was found when assaying extracts of the two type strains. Nucleoside hydrolase and dihydropyrimidine dehydrogenase levels in P. vesicularis were influenced by carbon source while dihydropyrimidinase activity was observed to increase after P. diminuta growth on dihydrothymine as a nitrogen source.
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Olson Biochemistry Laboratories, Department of Chemistry, South Dakota State University, Brookings 57007.
D000581:Amidohydrolases / Q000378:metabolism; D043525:Cytosine Deaminase; D004269:DNA, Bacterial / Q000235:genetics; D042943:Dihydrouracil Dehydrogenase (NADP); D006867:Hydrolases / Q000378:metabolism; D009700:Nucleoside Deaminases / Q000378:metabolism; D010088:Oxidoreductases / Q000378:metabolism; D011549:Pseudomonas / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D011741:Pyrimidine Nucleosides / Q000378:metabolism*; D011743:Pyrimidines / Q000378:metabolism*; D012689:Sequence Homology, Nucleic Acid
D004269:DNA, Bacterial; D011741:Pyrimidine Nucleosides; D011743:Pyrimidines; D010088:Oxidoreductases; D042943:Dihydrouracil Dehydrogenase (NADP); D006867:Hydrolases; D000581:Amidohydrolases; C020047:dihydropyrimidinase; D009700:Nucleoside Deaminases; D043525:Cytosine Deaminase