Studies of oxidative stress in cellular systems. The interaction of monocytes and erythrocytes.
1H spin echo NMR spectroscopy is used to follow the interaction of intact and viable erythrocytes and monocytes obtained from different sources in mixed cultures. After a lag time (270 min) erythrocyte glutathione is observed to become more oxidised. This result is believed to occur as a consequence of monocyte activation generating hydrogen peroxide or hypochlorous acid, which is targeted at the erythrocyte. The red cell in turn employs its sulphydryl system as an anti-oxidant defence.
McGowan
P E
PE
Department of Pure and Applied Chemistry, Strathclyde University, Glasgow, UK.
Reglinski
J
J
Smith
W E
WE
Wilson
R
R
Sturrock
R D
RD
D004912:Erythrocytes / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D009000:Monocytes / Q000378:metabolism*; D010084:Oxidation-Reduction