New chromosome 21 DNA markers isolated by pulsed field gel electrophoresis from an ETS2-containing Down syndrome chromosomal region.
To generate new chromosome 21 markers in a region that is critical for the pathogenesis of Down syndrome (D21S55-MX1), we used pulsed field gel electrophoresis (PFGE) to isolate a 600-kb NruI DNA fragment from the WA17 hybrid cell line, which has retained chromosome 21 as the only human material. This fragment, which contains the oncogene ETS2, was used to construct a partial genomic library. Among the 14 unique sequences that were isolated, 3 were polymorphic markers and contained sequences that are conserved in mammals. Five of these markers mapped on the ETS2-containing NruI fragment and allowed us to define an 800-kb high-resolution PFGE map.
Yaspo
M L
ML
Laboratoire de Biochimie-Génétique URA 1335 CNRS, Hôpital Necker, Paris, France.
Crété
N
N
Chettouh
Z
Z
Blouin
J L
JL
Rahmani
Z
Z
Stehelin
D
D
Sinet
P M
PM
Créau-Goldberg
N
N
Delabar
J M
JM
D000818:Animals; D015139:Blotting, Southern; D002874:Chromosome Mapping; D002891:Chromosomes, Human, Pair 21; D006224:Cricetinae; D004247:DNA; D004314:Down Syndrome / Q000235:genetics*; D016521:Electrophoresis, Gel, Pulsed-Field; D005819:Genetic Markers; D006801:Humans; D006822:Hybrid Cells; D051379:Mice; D009857:Oncogenes; D011110:Polymorphism, Genetic; D015682:Retroviridae Proteins, Oncogenic / Q000235:genetics
D005819:Genetic Markers; D015682:Retroviridae Proteins, Oncogenic; C050379:oncogene proteins v-ets; D004247:DNA