A direct radioimmunoassay for 5 alpha-androstane-3 alpha,17 beta-diol 17-glucuronide.
Synthesis of the 11 alpha-hemiglutaryl derivative of 5 alpha-androstane-3 alpha,17 beta-diol 17-glucuronide (androstane-diol-17G) starting from androsta-4,9(11)-diene-3,17-dione through a 10-step sequence and the preparation of its bovine serum albumin conjugate is described. By using this conjugate, antiserum was raised in rabbits which proved to be very specific for androstanediol-17G. A direct radioimmunoassay using a double antibody procedure is described for the measurement of androstanediol-17G from plasma without prior chromatography.
Rao
P N
PN
Department of Organic Chemistry, Southwest Foundation for Biomedical Research, San Antonio, Texas 78228-0147.
Rodriguez
A M
AM
Moore
P H
PH
Cessac
J W
JW
D015113:Androstane-3,17-diol / Q000031:analogs & derivatives* / Q000097:blood / Q000276:immunology; D000918:Antibody Specificity; D000941:Antigens / Q000276:immunology; D006801:Humans; D007106:Immune Sera / Q000096:biosynthesis / Q000276:immunology; D011863:Radioimmunoassay / Q000379:methods*
D000941:Antigens; D007106:Immune Sera; D015113:Androstane-3,17-diol; C028895:androstane-3,17-diol glucuronide