Two methyl ester derivatives of microcystins, cyclic heptapeptide hepatotoxins, isolated from Anabaena flos-aquae strain CYA 83/1.
Cultured cells of Anabaena flos-aquae strain CYA 83/1, isolated from Lake Edlandsvatn, Norway, produced two microcystin mono-methyl ester derivatives (1 and 2) at the D-Glu unit in addition to microcystin-LR (3), [D-Asp3]microcystin-LR (4), microcystin-RR (5), and [D-Asp3]microcystin-RR (6). Structures of these compounds were assigned based on their amino acid analysis with a Waters Pico Tag HPLC system plus fast atom bombardment mass spectrometry (FABMS), including tandem FABMS, analysis on the two new microcystins, [D-Glu(OCH3)6]microcystin-LR (1) and [D-Asp3, D-Glu(OCH3)6]microcystin-LR (2). Toxicity data were not obtained for 1 and 2 because of the small amounts isolated from the cells.
Sivonen
K
K
Department of Microbiology, University of Helsinki, Finland.
Skulberg
O M
OM
Namikoshi
M
M
Evans
W R
WR
Carmichael
W W
WW
Rinehart
K L
KL
D000595:Amino Acid Sequence; D017033:Anabaena / Q000737:chemistry*; D000818:Animals; D056486:Chemical and Drug Induced Liver Injury / Q000209:etiology; D002851:Chromatography, High Pressure Liquid; D005260:Female; D008387:Marine Toxins; D051379:Mice; D052998:Microcystins; D008969:Molecular Sequence Data; D010456:Peptides, Cyclic / Q000032:analysis* / Q000633:toxicity; D016339:Spectrometry, Mass, Fast Atom Bombardment
D008387:Marine Toxins; D052998:Microcystins; D010456:Peptides, Cyclic; C063855:microcystin RR; C057862:cyanoginosin LR