Characterization of cultured chemoreceptor cells dissociated from adult rabbit carotid body.
Short-term cell cultures were obtained from enzymatically dissociated carotid bodies from adult rabbits, and morphological and functional characterization of the cultured chemoreceptor cells were carried out. Under phase contrast, freshly isolated type I cells are round, bright, and 10-14 microns in diameter and exhibit strong fluorescence when stained with the glyoxylic acid technique. The content of endogenous dopamine in the cultures increased from 80 pmol/10(5) cells 2 h after plating the cells to 200 pmol/10(5) cells on the 3rd day, and the rate of synthesis and storage of [3H]dopamine from the precursor [3H]tyrosine increased from 1.7 pmol.10(5) cells-1.h-1 in 1-day cultures to 4 pmol.10(5) cells-1.h-1 on the 3rd day; the later values represent 80-85% of the expected values for the intact carotid body. After labeling with [3H]tyrosine, cultured chemoreceptor cells release [3H]dopamine when challenged by hypoxia, high external K+, or the protonophore dinitrophenol, the pattern of response being similar to that of the intact carotid body. When studied by whole cell clamp recording, individual chemoreceptor cells exhibit a marked variability in the properties of some ionic currents; the data, however, do not support the existence of distinct subpopulations of type I cells.
Pérez-García
M T
MT
Departamento de Bioquímica y Biología Molecular y Fisiología, Facultad de Medicina, Universidad de Valladolid, Spain.
Obeso
A
A
López-López
J R
JR
Herreros
B
B
González
C
C
D000818:Animals; D002344:Carotid Body / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D002478:Cells, Cultured; D002628:Chemoreceptor Cells / Q000502:physiology*; D003470:Culture Media; D004298:Dopamine / Q000378:metabolism; D004594:Electrophysiology; D000860:Hypoxia / Q000378:metabolism; D008856:Microscopy, Fluorescence; D011188:Potassium / Q000502:physiology; D011817:Rabbits
D003470:Culture Media; D011188:Potassium; D004298:Dopamine