Tyrosinase-like activity and estradiol binding in rat uterine nuclear extracts.
Nuclear extracts from the uteri of estradiol-implanted rats contain a tyrosinase-like enzyme that has three activities: monophenolase or cresolase, diphenolase or catecholase, and estrogen binding. When [3H]estradiol was used as a substrate, 3H2O was released from the A ring in the presence of copper and ascorbic acid. The optimal concentrations of these cofactors for the cresolase activity were established. The cresolase activity was lost on attempts at further purification. Estradiol binding was observed in conjunction with the enzymatic activity and was dependent on the presence of ascorbic acid and copper. The most potent inhibitors of 3H2O release from [3H]estradiol were those with a dihydroxyphenol moiety. The reaction was also sensitive to sulfhydryl reagents. These features of the enzyme are distinctive from other oxidases capable of attacking the aromatic ring of estrogens.
Garai
J
J
Department of Cell Biology, Baylor College of Medicine, Houston, TX 77030.
Clark
J H
JH
D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology; D001667:Binding, Competitive; D002467:Cell Nucleus / Q000201:enzymology*; D003300:Copper / Q000494:pharmacology; D004295:Dihydroxyphenylalanine / Q000378:metabolism; D004958:Estradiol / Q000378:metabolism*; D005260:Female; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D014442:Monophenol Monooxygenase / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013439:Sulfhydryl Reagents / Q000494:pharmacology; D014316:Tritium; D014599:Uterus / Q000201:enzymology* / Q000648:ultrastructure; D014867:Water / Q000378:metabolism
D013439:Sulfhydryl Reagents; D014867:Water; D014316:Tritium; D004958:Estradiol; D004295:Dihydroxyphenylalanine; D003300:Copper; D014442:Monophenol Monooxygenase; D001205:Ascorbic Acid