Inhibition of smooth muscle myosin's activity and assembly by an anti-rod monoclonal antibody.
Monoclonal antibodies specific for the rod region can affect smooth muscle myosin's motor properties. Actin movement by phosphorylated myosin was inhibited by an antibody (LMM.4) which binds to the COOH-terminal end of the coiled-coil rod, a region thought to be involved in filament assembly. The actin-activated ATPase activity of the myosin-antibody LMM.4 complex was also reduced 10-fold at actin concentrations that gave maximal turnover rates with filamentous myosin. Metal-shadowing of the phosphorylated myosin-antibody complex at low ionic strength showed small bundles of parallel extended molecules, instead of filaments. Five other anti-rod antibodies had little or no effect on myosin's ability to act as a motor. This is the first demonstration that a muscle myosin's activity is affected by its state of assembly. A common theme that emerges from the studies on both muscle and non-muscle myosins is that assembly into a filamentous structure stimulates the activity of the individual myosin molecules.
Horowitz
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Rosenstiel Basic Medical Sciences Research Center, Brandeis University, Waltham, Massachusetts 02254.
Trybus
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KM
D000199:Actins / Q000378:metabolism; D000205:Actomyosin / Q000378:metabolism; D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel; D005895:Gizzard, Avian / Q000378:metabolism; D015151:Immunoblotting; D007700:Kinetics; D008854:Microscopy, Electron; D009130:Muscle, Smooth / Q000378:metabolism*; D009218:Myosins / Q000302:isolation & purification / Q000378:metabolism* / Q000648:ultrastructure; D010766:Phosphorylation; D014422:Turkeys
D000199:Actins; D000911:Antibodies, Monoclonal; D000205:Actomyosin; D009218:Myosins