Interaction of antimicrobial dermaseptin and its fluorescently labeled analogues with phospholipid membranes.
Dermaseptin, a 34 amino-acid residue antimicrobial polypeptide [Mor, A., Nguyen, V. H., Delfour, A., Migliore-Samour, D., & Nicolas, P. (1991) Biochemistry 30, 8824-8830] was synthesized and selectively labeled at its N-terminal amino acid with either 7-nitrobenz-2-oxa-1,3-diazole-4-yl (NBD), rhodamine, or fluorescein. The fluorescent emission spectra of the NBD-labeled dermaseptin displayed a blue-shift upon binding to small unilamellar vesicles (SUV), reflecting the relocation of the fluorescent probe to an environment of increased apolarity. Titrations of solutions containing NBD-labeled dermaseptin with SUV composed of zwitterionic or acidic phospholipids were used to generate binding isotherms, from which were derived surface partition constants of (0.66 +/- 0.06) x 10(4) M-1 and (2.8 +/- 0.3) x 10(4) M-1, respectively. The shape of the binding isotherms, as well as fluorescence energy transfer measurements, suggests that some aggregation of membrane-bound peptide monomers occurs in acidic but not in zwitterionic vesicles. The preferential susceptibility of the peptide to proteolysis when bound to zwitterionic but not to acidic SUV suggests that these aggregates might then penetrate a relatively short distance into the hydrophobic region of the acidic membrane. Furthermore, the results provide good correlation between the peptide's strong binding and its ability to permeate membranes composed of acidic phospholipids, as revealed by a dissipation of diffusion potential and a release of entrapped calcein from SUV.
Pouny
Y
Y
Department of Membrane Research and Biophysics, Weizmann Institute of Science, Rehovot, Israel.
Rapaport
D
D
Mor
A
A
Nicolas
P
P
Shai
Y
Y
D000595:Amino Acid Sequence; D029845:Amphibian Proteins; D000890:Anti-Infective Agents / Q000378:metabolism*; D023181:Antimicrobial Cationic Peptides; D004058:Diffusion; D005452:Fluoresceins / Q000378:metabolism; D005456:Fluorescent Dyes; D008051:Lipid Bilayers / Q000378:metabolism*; D008969:Molecular Sequence Data; D010455:Peptides / Q000378:metabolism*; D010539:Permeability; D010743:Phospholipids / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013816:Thermodynamics; D014634:Valinomycin
D029845:Amphibian Proteins; D000890:Anti-Infective Agents; D023181:Antimicrobial Cationic Peptides; D005452:Fluoresceins; D005456:Fluorescent Dyes; D008051:Lipid Bilayers; D010455:Peptides; D010743:Phospholipids; C070905:dermaseptin; D014634:Valinomycin; C007740:fluorexon