title: Pulsed magnetic field effects on calcium signaling in lymphocytes: dependence on cell status and field intensity.
abstract: The effect of 3-Hz, monopolar, quasi-rectangular magnetic field pulses on 45Ca2+ uptake in resting and mitogen-treated rat thymic lymphocytes was evaluated. A 30-min, non-thermal exposure to the pulsed magnetic field (Bpeak = 6.5 mT, Emax = 0.69 mV/cm, Jmax = 2.6 microA/cm2) reduced Concanavalin A-induced 45Ca2+ uptake by 45%. It was observed that (i) the induction of the 3-Hz field response depended on Ca2+ signal transduction activation; (ii) the response direction (stimulation or inhibition) depended on the level of lymphocyte mitogen responsiveness, and (iii) the field response magnitude increased with increasing magnetic field flux densities (Bpeak = 0, 1.6, 6.5 and 28 mT). Our results demonstrate field effects at Bmax nearly 10(4) greater than that of the average human environment for low-frequency magnetic fields and they are consistent with the independent results from other 3-Hz pulsed magnetic field studies with lymphocytes.
authors: Center for Functional Imaging, Lawrence Berkeley Laboratory, University of California, Berkeley 94720.
authors: J
authors: J
authors: Walleczek
authors: T F
authors: TF
authors: Budinger
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D003208:Concanavalin A; D004307:Dose-Response Relationship, Radiation; D004574:Electromagnetic Fields; D008214:Lymphocytes / Q000378:metabolism / Q000528:radiation effects*; D051381:Rats; D015398:Signal Transduction / Q000528:radiation effects*
chemical_list: D003208:Concanavalin A; D002118:Calcium