[Chemical and serological study of lipopolysaccharide isolated from Shigella flexneri 88-893 possessing a new type-antigen].
The O-specific polysaccharide chain which represents a new type-antigen in lipopolysaccharide (LPS) of Shigella flexneri 88-893 was investigated. The O-polysaccharide chain was found to be composed of repeating units comprising rhamnose, N-acetylglucosamine and glucose (3:1:2). In the passive hemolysis test, group-6 antiserum of S. flexneri exhibited a high hemolytic titer (50% hemolysis titer: 7,900) against sheep red blood cells (SRBC) sensitized with intact 893 LPS, but virtually no hemolytic activity against SRBC sensitized with alkali-treated 893 LPS. None of the type-specific antisera (I-VI), showed any significant hemolytic titer against SRBC sensitized with either intact or alkali-treated 893 LPS. Thus, 893 LPS contained both the group-6 antigen and a new type-antigen which is distinct from any known type-antigen of S. flexneri.
Toshima
Y
Y
Department of Microbiology, School of Pharmaceutical Sciences, Josai University, Saitama, Japan.
Iguchi
T
T
Kondo
S
S
Matsushita
S
S
Kudoh
Y
Y
Hisatsune
K
K
D000818:Animals; D000942:Antigens, Bacterial / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification*; D002241:Carbohydrates / Q000032:analysis; D006461:Hemolysis; D008070:Lipopolysaccharides / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification*; D012703:Serotyping; D012763:Shigella flexneri / Q000145:classification* / Q000276:immunology
D000942:Antigens, Bacterial; D002241:Carbohydrates; D008070:Lipopolysaccharides