Evidence for a structurally homologous Rh-like polypeptide in Rhnull erythrocytes.
Human Rhnull red blood cells fail to react with Rh antibodies, indicating that these cells are either devoid of Rh protein or, like other species, possess antigenically distinct variants. To determine whether Rhnull cells possess an Rh-like polypeptide, 32-kDa proteins from D--, rr, and Rhnull cells were labeled with the cysteine-specific probe, 125I-labeled pyridyldithioethylamine. Size comparisons of labeled proteins in Triton X-100-solubilized membranes from Rh-bearing and Rhnull cells showed similar sedimentation coefficients and Stoke's radii. Immunoprecipitated Rh(D) from D-- cells, Rh(c) from rr cells, and purified 32-kDa proteins from Rhnull cells were digested with alpha-chymotrypsin and examined by high-performance liquid chromatography and by two-dimensional iodopeptide mapping. Analysis of 125I-labeled chymotryptic fragments from immunoprecipitated Rh(D) and Rh(c) showed the labeled peptides from both phenotypes to be virtually identical. High-performance liquid chromatography profiles and iodopeptide maps of 32-kDa Rhnull proteins yielded patterns identical to 32-kDa proteins isolated from D-- cells and rr cells with the exception of one missing 125I-labeled peptide. Further analysis of the Rh-related fragments from Rhnull cells showed significant homology with immunoprecipitated Rh(D) and Rh(c). DNA sequence analysis of cysteine-encoding regions from Rh-bearing and Rhnull cells showed complete identity. These data suggest that Rhnull red blood cells, although serologically distinct, possess an Rh-like protein that is structurally very similar to Rh(D) and Rh(c).
Connor
J
J
Department of Cell Biology, University of Texas M.D. Anderson Cancer Center, Houston 77030.
Bar-Eli
M
M
Gillum
K D
KD
Schroit
A J
AJ
D000595:Amino Acid Sequence; D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000097:blood / Q000235:genetics / Q000302:isolation & purification; D004220:Disulfides / Q000097:blood; D015180:Electrophoresis, Gel, Two-Dimensional; D004910:Erythrocyte Membrane / Q000378:metabolism*; D006801:Humans; D007457:Iodine Radioisotopes; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009838:Oligodeoxyribonucleotides; D010446:Peptide Fragments / Q000302:isolation & purification; D011725:Pyridines / Q000097:blood; D012091:Repetitive Sequences, Nucleic Acid; D012204:Rh-Hr Blood-Group System / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology*
D004220:Disulfides; D007457:Iodine Radioisotopes; D009838:Oligodeoxyribonucleotides; D010446:Peptide Fragments; D011725:Pyridines; D012204:Rh-Hr Blood-Group System; C055639:pyridyldithioethylamine; D004247:DNA