title: Comparison of excretory/secretory and circulating antigens of Toxoplasma gondii by enzyme immunoassay and immunoblotting.
abstract: Toxoplasma gondii trophozoites (RH strain) were cultured in embryonic fibroblasts in order to study the kinetics of production of excretory/secretory antigens, and the results were compared to the production of circulating antigens in an in vivo mouse model. By capture-ELISA, excretory/secretory antigens were first detected on the fourth day of culture whereas circulating antigens were first detected 1 day after infection. Similar concentrations of antigens were detected in both models as evidenced by comparable absorbance values. By immunoblotting, the excretory/secretory antigens were also detected later compared to circulating antigens (day 4 vs day 1). Seven major polypeptides were detected in both antigen preparations, six of them having the same molecular mass (110, 75, 48, 30, 24 and 22 kDa).
authors: Laboratoire de Parasitologie, Hôpital Nord, Saint-Etienne, Saint-Priest-en-Jarez, France.
authors: J
authors: J
authors: Hafid
authors: H
authors: H
authors: Raberin
authors: B
authors: B
authors: Pozzetto
authors: Z Y
authors: ZY
authors: Akono
authors: R T
authors: RT
authors: Sung
authors: M
authors: M
authors: Jana
authors: O
authors: O
authors: Gaudin
mesh_terms: D000818:Animals; D000953:Antigens, Protozoan / Q000032:analysis* / Q000096:biosynthesis; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005347:Fibroblasts / Q000469:parasitology; D006801:Humans; D015151:Immunoblotting; D051379:Mice; D014122:Toxoplasma / Q000254:growth & development / Q000276:immunology*; D014124:Toxoplasmosis, Animal / Q000276:immunology*
chemical_list: D000953:Antigens, Protozoan