Protection to glycolysis by a combination of 5-hydroxy-L-tryptophan and 2-aminoethylisothiuronium bromide hydrobromide in lethally irradiated rats.
Rate of glycolysis in vivo at different time intervals following 8 Gy [LD100(30)] whole body gamma radiation (WBGR) was evaluated by estimating liver glycogen, blood sugar, serum lactic dehydrogenase (LDH) and blood lactic acid concentration in adult male Sprague Dawley rats. Within 1 hr of radiation exposure, a significant fall in liver glycogen was observed in rats fed food and water ad libitum. The glycogen content increased after 24 hr and had returned to control level on 7th day after radiation exposure. Blood sugar, serum LDH and blood lactate levels increased significantly as compared to non irradiated controls. Pretreatment with 5-hydroxy-L-tryptophan (5-HTP; 100 mg/kg) + 2-aminoethylisothiuronium bromide hydrobromide (AET; 20 mg/kg) ip 30 min before 8 Gy WBGR, modified these values and restored them to normal level on 7th day post-irradiation.
Basu
S K
SK
Radiation Biology Department, Institute of Nuclear Medicine & Allied Sciences, Delhi, India.
Srinivasan
M N
MN
Chuttani
K
K
George
S
S
D006916:5-Hydroxytryptophan / Q000008:administration & dosage*; D000818:Animals; D005720:Gamma Rays; D006019:Glycolysis / Q000187:drug effects* / Q000528:radiation effects*; D008112:Liver Glycogen / Q000378:metabolism / Q000528:radiation effects; D008297:Male; D011837:Radiation-Protective Agents / Q000008:administration & dosage; D051381:Rats; D017207:Rats, Sprague-Dawley; D000338:beta-Aminoethyl Isothiourea / Q000008:administration & dosage*
D008112:Liver Glycogen; D011837:Radiation-Protective Agents; D000338:beta-Aminoethyl Isothiourea; D006916:5-Hydroxytryptophan