title: A study of the development of endotoxin-induced inflammation in the bovine teat.
abstract: Endotoxin-induced local inflammation was studied by frequent samplings in a bovine teat cistern model, which provides a unique possibility for in vivo studies of reactions in the teat without interference from the mammary gland. A rapid inflammatory response of rather short duration was elicited after endotoxin administration. An initial increase in the concentrations of bovine serum albumin and N-acetyl-beta-D-glucosaminidase, indicating a disturbance in the epithelial integrity, was observed between 1 and 1.5 h post infusion (p.i.). Approximately 0.5 h later, the first influx of leukocytes, mainly neutrophils, appeared. The neutrophils tended to enter the teat cistern in several peaks occurring between 2.5 and 5 h p.i. The sampling procedure decreased the accumulation of cells by approximately 40%, which was probably due to the removal of inflammatory mediators at an early stage. The parallel use of 2 teats instead of 1 had no major influence on the inflammatory process. This teat cistern model and the experimental procedure used should be suitable for further studies of the development of local inflammation.
authors: Department of Obstetrics and Gynaecology, Faculty of Veterinary Medicine, Swedish University of Agricultural Sciences, Uppsala.
authors: K
authors: K
authors: Persson
authors: C H
authors: CH
authors: Sandgren
mesh_terms: D000118:Acetylglucosaminidase / Q000378:metabolism; D000818:Animals; D002417:Cattle; D004731:Endotoxins / Q000633:toxicity*; D005260:Female; D007958:Leukocyte Count; D008414:Mastitis, Bovine / Q000139:chemically induced / Q000276:immunology* / Q000378:metabolism; D012710:Serum Albumin, Bovine / Q000378:metabolism
chemical_list: D004731:Endotoxins; D012710:Serum Albumin, Bovine; D000118:Acetylglucosaminidase