title: Cloning of DNA from a Rhodococcus strain conferring the ability to decolorize sulfonated azo dyes.
abstract: Azo dyes are recalcitrant pollutants. Two sulfonated azo dyes, Orange II and Amido black, are effectively decolorized by certain nocardioform strains of the genus Rhodococcus. A mutant of one of these strains was isolated which had lost azo-dye decolorizing ability and the strain was used to clone DNA conferring this ability, by screening a BclI library constructed from DNA of a decolorizing strain. The relevant genetic information was located on a 6.3-kb fragment of DNA.
authors: Department of Genetics, University of the Witwatersrand, Johannesburg, South Africa.
authors: G S
authors: GS
authors: Heiss
authors: B
authors: B
authors: Gowan
authors: E R
authors: ER
authors: Dabbs
mesh_terms: D000580:Amido Black / Q000378:metabolism*; D001391:Azo Compounds / Q000378:metabolism*; D001557:Benzenesulfonates / Q000378:metabolism*; D001673:Biodegradation, Environmental; D003001:Cloning, Molecular; D004396:Coloring Agents / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics*; D016296:Mutagenesis; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism; D009601:Nitroreductases; D015183:Restriction Mapping; D012240:Rhodococcus / Q000235:genetics* / Q000378:metabolism*
chemical_list: D001391:Azo Compounds; D001557:Benzenesulfonates; D004396:Coloring Agents; D004269:DNA, Bacterial; D009247:NADH, NADPH Oxidoreductases; D009601:Nitroreductases; C021106:azoreductase; C014722:2-naphthol orange; D000580:Amido Black