title: Effect of concanavalin A on the activity of acid and alkaline phosphatases.
abstract: The effect of Concanavalin A on the activity of acid and alkaline phosphatases has been investigated. Both the glycoproteins were inhibited by Con A (about 30-40% with 4 mg final concentration in the reaction mixture of Con A). In case of the acid phosphatase, the addition of 1.0 M NaCl, instead of 0.5 M, stimulated the enzyme activity at low concentrations of Con A. The significance of the lectin effect on the stability of glycoenzymes and its implications in bioseparation and enzyme immobilization is briefly discussed.
authors: Department of Chemistry, Indian Institute of Technology, Delhi.
authors: S
authors: S
authors: Ahmad
authors: M N
authors: MN
authors: Gupta
mesh_terms: D000135:Acid Phosphatase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D000818:Animals; D002417:Cattle; D002645:Chickens; D003208:Concanavalin A / Q000494:pharmacology*; D004789:Enzyme Activation; D004795:Enzyme Stability; D004800:Enzymes, Immobilized; D006358:Hot Temperature; D007422:Intestines / Q000201:enzymology
chemical_list: D004800:Enzymes, Immobilized; D003208:Concanavalin A; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase