title: Immune responses to dietary antigens in gluten-sensitive enteropathy of Irish setters.
abstract: This study examined whether increased intestinal permeability in Irish setters with gluten-sensitive enteropathy was associated with altered immune responses to ingested antigens, and whether a humoral immune response to gluten is involved in the pathogenesis of the condition. Affected setters reared on a wheat-containing diet were compared with littermates reared on a cereal-free diet and age-matched control setters. Measurement of serum immunoglobulins revealed increased serum IgA concentrations in affected dogs. Antibody responses to a variety of dietary antigens were measured by ELISA. Antibody levels to ovalbumin, collagen I and II and soya were not significantly different from normal dogs. Anti-gliadin antibody concentrations were lower in affected dogs than controls, and correlated with immune complex formation as assessed by C1q binding. The study suggested a modified immune response in dogs with increased intestinal permeability, although there was no major systemic antibody response to dietary antigens.
authors: Department of Veterinary Pathology, University of Liverpool.
authors: E J
authors: EJ
authors: Hall
authors: S D
authors: SD
authors: Carter
authors: A
authors: A
authors: Barnes
authors: R M
authors: RM
authors: Batt
mesh_terms: D000818:Animals; D000906:Antibodies / Q000097:blood; D000941:Antigens / Q000276:immunology*; D015153:Blotting, Western / Q000662:veterinary; D002446:Celiac Disease / Q000276:immunology / Q000662:veterinary*; D004044:Dietary Proteins / Q000276:immunology*; D004283:Dog Diseases / Q000276:immunology*; D004285:Dogs; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D005983:Glutens / Q000276:immunology*; D007413:Intestinal Mucosa / Q000378:metabolism; D010539:Permeability
chemical_list: D000906:Antibodies; D000941:Antigens; D004044:Dietary Proteins; D005983:Glutens