Bone morphogenetic proteins (BMP-2 and BMP-3) induce the late phase expression of the proto-oncogene c-fos in murine osteoblastic MC3T3-E1 cells.
Here we report that bone morphogenetic proteins 2 and 3 (BMP-2 and BMP-3) induced marked expression of c-fos mRNA in a biphasic manner, i.e. the late phase (48 to 60 h) as well as the immediate-early phase (0.5 h), in murine osteoblastic MC3T3-E1 cells in vitro. The BMP-induced late phase c-fos gene expression was temporally associated with the onset of marked expression of the genes for osteocalcin and alkaline phosphatase, differentiation markers of mature osteoblasts. In contrast, none of TGF-beta 1, 10% FBS, IGF-I and IGF-II, which induced only the immediate-early c-fos mRNA expression, stimulated the expression of osteocalcin and alkaline phosphatase genes. These data suggest that in osteoblasts BMP-2 and BMP-3 induce the late phase expression of c-fos, which may play a role in transcriptional activation of the genes involved in differentiation of osteoblasts.
Ohta
S
S
Department of Orthopaedic Surgery, Kyoto University Faculty of Medicine, Japan.
Hiraki
Y
Y
Shigeno
C
C
Suzuki
F
F
Kasai
R
R
Ikeda
T
T
Kohno
H
H
Lee
K
K
Kikuchi
H
H
Konishi
J
J
D000818:Animals; D055398:Bone Morphogenetic Protein 3; D019485:Bone Morphogenetic Proteins; D002417:Cattle; D002454:Cell Differentiation / Q000235:genetics / Q000502:physiology; D002460:Cell Line; D005786:Gene Expression Regulation; D006133:Growth Substances / Q000502:physiology*; D051379:Mice; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism*; D011506:Proteins / Q000502:physiology*; D016760:Proto-Oncogene Proteins c-fos / Q000096:biosynthesis / Q000235:genetics*
D055398:Bone Morphogenetic Protein 3; D019485:Bone Morphogenetic Proteins; D006133:Growth Substances; D011506:Proteins; D016760:Proto-Oncogene Proteins c-fos