Nonradioactive southwestern analysis using chemiluminescent detection.
Replacing radioactively labeled probes by nonradioactive ones and detection by chemiluminescence instead of colorimetry allows a nonhazardous handling and offers the possibility of easily reprobing filters in Southwestern analysis. Using the described procedure, we were able to determine the molecular weight of DNA-binding proteins and achieve a high signal-to-noise ratio.
Dooley
S
S
Institute of Human Genetics, University of Saarland, Homburg, FRG.
Welter
C
C
Blin
N
N
D000818:Animals; D015153:Blotting, Western; D003124:Colorimetry; D004268:DNA-Binding Proteins / Q000032:analysis; D003856:Deoxyuracil Nucleotides; D004076:Digoxigenin / Q000031:analogs & derivatives; D004591:Electrophoresis, Polyacrylamide Gel; D005347:Fibroblasts / Q000737:chemistry; D006801:Humans; D015151:Immunoblotting / Q000379:methods*; D008163:Luminescent Measurements; D008567:Membranes, Artificial; D051379:Mice; D015345:Oligonucleotide Probes; D011145:Polyvinyls; D005465:Fluorocarbon Polymers
D004268:DNA-Binding Proteins; D003856:Deoxyuracil Nucleotides; D004076:Digoxigenin; D008567:Membranes, Artificial; D015345:Oligonucleotide Probes; D011145:Polyvinyls; C070202:digoxigenin-11-deoxyuridine triphosphate; C024865:polyvinylidene fluoride; D005465:Fluorocarbon Polymers