title: Interaction of U6 snRNA with a sequence required for function of the nematode SL RNA in trans-splicing.
abstract: Nematode trans-spliced leader (SL) RNAs are composed of two domains, an exon [the 22-nucleotide spliced leader] and a small nuclear RNA (snRNA)-like sequence. Participation in vitro of the spliced leader RNA in trans-splicing reactions is independent of the exon sequence or size and instead depends on features contained in the snRNA-like domain of the molecule. Chemical modification interference analysis has revealed that two short sequence elements in the snRNA-like domain are necessary for SL RNA activity. These elements are sufficient for such activity because when added to a 72-nucleotide fragment of a nematode U1 snRNA, this hybrid RNA could participate in trans-splicing reactions in vitro. One of the critical sequence elements may function by base-pairing with U6 snRNA, an essential U snRNA for both cis- and trans-splicing.
authors: Department of Molecular Biology and Microbiology, Case Western Reserve University School of Medicine, Cleveland, OH 44106.
authors: G J
authors: GJ
authors: Hannon
authors: P A
authors: PA
authors: Maroney
authors: Y T
authors: YT
authors: Yu
authors: G E
authors: GE
authors: Hannon
authors: T W
authors: TW
authors: Nilsen
mesh_terms: D000818:Animals; D001200:Ascaris / Q000235:genetics*; D001483:Base Sequence; D001665:Binding Sites; D005091:Exons; D008961:Models, Structural; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012326:RNA Splicing; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D012342:RNA, Small Nuclear / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D012342:RNA, Small Nuclear