Interaction of rhodopsin, G-protein and kinase in octopus photoreceptors.
Light induced phosphorylation of octopus rhodopsin was greatly enhanced by guanosine 5'-O-(3-thiotriphosphate) (GTP gamma S), suggesting that the kinases are involved in regulating interaction between rhodopsin and G-protein. We determined phosphorylated peptides of octopus rhodopsin in the presence or absence of GTP gamma S. Possible phosphorylation sites for octopus rhodopsin enhanced by GTP gamma S were Thr329, Thr330 and/or Thr336, which suggest that the G-protein associates with cytoplasmic loops including C-terminal peptide in the seventh helix of octopus rhodopsin.
Tsuda
M
M
Department of Life Science, Himeji Institute of Technology, Hyogo, Japan.
Hirata
H
H
Tsuda
T
T
D000595:Amino Acid Sequence; D000818:Animals; D005136:Eye Proteins; D051606:G-Protein-Coupled Receptor Kinase 1; D019204:GTP-Binding Proteins / Q000378:metabolism; D008969:Molecular Sequence Data; D049831:Octopodiformes; D010766:Phosphorylation; D010777:Photochemistry; D010786:Photoreceptor Cells / Q000378:metabolism* / Q000528:radiation effects; D011494:Protein Kinases / Q000378:metabolism; D012243:Rhodopsin / Q000737:chemistry / Q000378:metabolism*
D005136:Eye Proteins; D012243:Rhodopsin; D011494:Protein Kinases; D051606:G-Protein-Coupled Receptor Kinase 1; D019204:GTP-Binding Proteins