Porcine dermal collagen as a wound dressing for skin donor sites and deep partial skin thickness burns.
Collagen was extracted by pepsin digestion from porcine skin, and collagen membrane was prepared by salt precipitation. The porcine collagen membrane was evaluated as a burn wound dressing in deep partial skin thickness burn wounds in rats. Burn wounds, 4 x 4 cm, were inflicted by exposure of skin to 75 degrees C for 15 s followed by de-epithelialization. Wound healing was assessed by planimetry of epithelialization on day 10 after injury. Open wounds exhibited 24 per cent of wound area re-epithelialized. Collagen membrane dressing significantly improved the healing to 69 per cent of wound area (P < 0.0001). In a completely separate experiment, the porcine collagen membrane was applied as a wound dressing to the donor sites of burn patients, and its effect on wound healing was compared with that of a petroleum jelly gauze dressing. The donor sites covered with petroleum jelly gauze had re-epithelialized by an average of 14.5 days (ranging from 13 to 16 days) after wounding. The wounds dressed with collagen membrane demonstrated a significant increase in the healing rate. Complete re-epithelialization was observed by 10.3 days (ranging from 10 to 12 days) after wounding (P < 0.0001).
Gao
Z R
ZR
Department of Burn Surgery, Affiliated Hospital of Qinghai Medical College, Xining, People's Republic of China.
Hao
Z Q
ZQ
Li
Y
Y
Im
M J
MJ
Spence
R J
RJ
D000293:Adolescent; D000328:Adult; D000818:Animals; D001684:Biological Dressings; D002056:Burns / Q000473:pathology / Q000628:therapy*; D002648:Child; D002675:Child, Preschool; D003094:Collagen / Q000627:therapeutic use*; D005260:Female; D006801:Humans; D008297:Male; D010577:Petrolatum; D051381:Rats; D017207:Rats, Sprague-Dawley; D016038:Skin Transplantation / Q000473:pathology; D014945:Wound Healing
D010577:Petrolatum; D003094:Collagen