Interleukin-1 alpha: regulation of cellular proliferation and collagen synthesis in cultured human osteoblast-like cells.
In this work the authors studied the effects of interleukin-1 alpha on metabolic activities of human osteoblast-like cells in vitro. The bone nature of the cells was established by assaying for specific bone protein, the osteonectin, and the parathormone receptor, an osteoblast marker. Administration of interleukin-1 alpha to cultured osteoblasts produce an increase in cellular proliferation as suggested by 3H-thymidine incorporation and cell growth studies. Interleukin-1 alpha also affected collagen synthesis confirming its potential role on bone-formation and resorption processes.
Bodo
M
M
Dipartimento di Medicina Sperimentale e Scienze Biochimiche, Università degli Studi Perugia, Italie.
Venti
G
G
Pezzetti
F
F
Ardisia
C
C
Antonica
A
A
Carinci
F
F
Becchetti
E
E
D000262:Adenylyl Cyclases / Q000378:metabolism; D000328:Adult; D002455:Cell Division / Q000187:drug effects*; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis*; D004247:DNA / Q000096:biosynthesis; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology*; D007930:Leucine / Q000378:metabolism; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D010281:Parathyroid Hormone / Q000494:pharmacology; D011433:Propranolol / Q000494:pharmacology; D014176:Protein Biosynthesis; D011994:Recombinant Proteins / Q000494:pharmacology; D013936:Thymidine / Q000378:metabolism; D014316:Tritium
D007375:Interleukin-1; D010281:Parathyroid Hormone; D011994:Recombinant Proteins; D014316:Tritium; D003094:Collagen; D004247:DNA; D011433:Propranolol; D000262:Adenylyl Cyclases; D007930:Leucine; D013936:Thymidine