Microinsemination of rabbit oocytes with heat-treated sperm: embryonic development.
A study was conducted to evaluate the ability of rabbit oocyte to develop to the pronuclear stage and subsequent development if injected with heat-treated sperm. The rate of fertilization of eggs after microinjection of cauda epididymal spermatozoa heated at 60 degrees C for 30 min was 36%; the rate of cleavage was 24%. The rate of fertilization after microinjection of heat-treated sperm nuclei was 43%; the rate of cleavage was 26%. On the other hand, the rate of fertilization of eggs injected with sperm without heat treatment was 43%, the rate of cleavage was 29%, and there was no difference between eggs microinjected with heat-treated spermatozoa or sperm nuclei and those not heat treated. Of the viable eggs injected with heat-treated sperm, 16% developed to the 6- to 8-cell stage 72 h after injection.
Hoshi
K
K
Department of Obstetrics and Gynecology, Fukushima Medical College, Japan.
Yazawa
H
H
Yanagida
K
K
Sato
A
A
D000818:Animals; D003470:Culture Media; D005314:Embryonic and Fetal Development; D005260:Female; D005307:Fertilization in Vitro; D006358:Hot Temperature; D008297:Male; D008845:Microinjections; D009865:Oocytes / Q000502:physiology*; D011247:Pregnancy; D011817:Rabbits; D013094:Spermatozoa / Q000502:physiology*
D003470:Culture Media