Localization of three novel hybrid breakpoints and refinement of 18 marker assignments in the human 3cen-p21.1 region.
Using the human/hamster cell line UCTP2A-3, we have generated and isolated three hybrids, each containing a novel human chromosome 3p break. All chromosome 3 materials distal to the breaks were lost. Two of the breakpoints were produced using aphidicolin induction; the third breakpoint occurred spontaneously. The aphidicolin-induced breaks were localized to 3p21.1 in hybrid AR1 and to p14.1 in hybrid AR2. The spontaneous break was localized to 3p11 in hybrid 2A-3-1. These hybrids were used to sublocalize 18 chromosome 3 probes further to five regions within 3cen-p21.1. The new hybrids and sublocalized markers will be useful in the study and characterization of the 3p11, 3p14.1, and 3p21.1 segments of chromosome 3.
Wang
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ND
Department of Molecular Biology and Genetics, Wayne State University School of Medicine, Detroit, Michigan 48201.
Testa
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JR
Smith
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D000818:Animals; D016590:Aphidicolin / Q000633:toxicity; D001483:Base Sequence; D002871:Chromosome Banding; D002893:Chromosomes, Human, Pair 3; D006224:Cricetinae; D004247:DNA; D005819:Genetic Markers; D006801:Humans; D006822:Hybrid Cells; D007621:Karyotyping; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction
D005819:Genetic Markers; D016590:Aphidicolin; D004247:DNA