Cytotoxicity of nickel oxide particles in rat alveolar macrophages cultured in vitro.
The cytotoxicity of two types of nickel oxide particles was investigated in rat alveolar macrophages cultured in vitro. The trypan blue dye exclusion test and the release of lactate dehydrogenase into culture medium were used as cytotoxic indices. Although both types of nickel oxide particles were produced by the same manufacturer and were commercially sold under the same name of "nickel oxide", one type of the nickel oxide particles which had black color was much more cytotoxic than the other type of green color. The black nickel oxide particles were more soluble in various kinds of solutions than the green nickel oxide particles. Therefore, it appears that the difference in the cytotoxicity of the black and green nickel oxide particles may be attributable to the difference in the solubility of the particles.
Takahashi
S
S
Division of Comparative Radiotoxicology, National Institute of Radiological Sciences, Chiba, Japan.
Yamada
M
M
Kondo
T
T
Sato
H
H
Furuya
K
K
Tanaka
I
I
D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D016676:Macrophages, Alveolar / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D009532:Nickel / Q000737:chemistry / Q000493:pharmacokinetics / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar; D012995:Solubility
D009532:Nickel; C028007:nickel monoxide; D007770:L-Lactate Dehydrogenase