title: [Metabolism of Ca2+ in myocytes and peroxidation products in ischemia-reperfusion injury under extracorporeal circulation].
abstract: The metabolism of calcium ion (Ca2+) in myocytes in ischemia-reperfusion injury under extracorporeal circulation (ECC) was studied by cytochemistry and electron microscopy. Ten mongrel dogs were under ECC with aortic cross-clamping for 120 minutes. A cold GIK crystalloid cardioplegic solution was infused via the aortic root intermittently during ischemia, and the myocardial temperature was maintained at 5-10 degrees C with ice slush. The morphological changes of Ca(2+)-ATPase activity in myocytes were estimated using the "lead citrate method". The activities of mitochondria, which had been temporarily decreased just before reperfusion, increased immediately after reperfusion and decreased again 60 minutes later. Electromicroscopy revealed swelling of mitochondria and laceration of myofibrils as well as intracellular edema 60 minutes after reperfusion. Immediately after reperfusion and 60 minutes later, creatine phosphokinase iso-enzyme (CK-MB) release in coronary sinus blood was significantly (p < 0.01) greater than that before the start of ECC. Anaerobic metabolism immediately after reperfusion was more active than that before aortic cross-clamping, as demonstrated by changes in excess lactate (delta XL) and redox potential (delta Eh) of lactate and pyruvate (delta XL, p < 0.05; delta Eh, p < 0.01). Thus, in ischemia-reperfusion injury, alterations of Ca(2+)-ATPase activity of mitochondria reflect the functional and morphological viability of the myocardium. Immediately and 60 minutes after reperfusion, the level of thiobarbituric acid was significantly (p < 0.01) higher and the level of alpha-tocopherol was significantly (p < 0.01) less than respective levels before the start of ECC.(ABSTRACT TRUNCATED AT 250 WORDS)
authors: Second Department of Surgery, Kyoto Prefectural University of Medicine, Japan.
authors: T
authors: T
authors: Kawai
authors: Y
authors: Y
authors: Wada
authors: H
authors: H
authors: Kawachi
authors: K
authors: K
authors: Ohga
authors: T
authors: T
authors: Oka
authors: M
authors: M
authors: Yasuda
authors: T
authors: T
authors: Yoshikawa
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D000252:Calcium-Transporting ATPases / Q000378:metabolism; D004285:Dogs; D005112:Extracorporeal Circulation; D005609:Free Radicals; D008929:Mitochondria, Heart / Q000201:enzymology / Q000648:ultrastructure; D015428:Myocardial Reperfusion Injury / Q000209:etiology / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism*; D017382:Reactive Oxygen Species; D013858:Thiobarbiturates / Q000378:metabolism; D014810:Vitamin E / Q000378:metabolism
chemical_list: D005609:Free Radicals; D017382:Reactive Oxygen Species; D013858:Thiobarbiturates; D014810:Vitamin E; D000252:Calcium-Transporting ATPases; C029684:thiobarbituric acid; D002118:Calcium