Investigation of the neighbour relationships between photosystem II polypeptides in the two types of isolated reaction centres (D1/D2/cytb559 and CP47/D1/D2/cyt b559 complexes).
The nearest neighbour relationships within the D1/D2/cyt b559 complex (PSIIRC) and the CP47/D1/D2/cyt b559 complex (RC-CP47) were investigated by using different length bifunctional crosslinking agents. The crosslinking products were identified by immunoblotting with polyclonal antibodies and by two-dimensional gel electrophoresis. Seven products (CP47/D2, D1/D2/alpha, D1/D2, D2/alpha, D1/alpha, alpha/alpha, alpha/beta) have been revealed in both complexes. The crosslinking of both complexes does not increase their photostability. The photocrosslinking products (D1/alpha and D2/alpha) appeared under illumination of complexes with light of high intensity.
Moskalenko
A A
AA
Institute of Soil Science and Photosynthesis RAS, Pushchino, Russian Federation.
Barbato
R
R
Giacometti
G M
GM
D001426:Bacterial Proteins / Q000737:chemistry; D003432:Cross-Linking Reagents; D003573:Cytochrome b Group / Q000737:chemistry*; D015180:Electrophoresis, Gel, Two-Dimensional; D015151:Immunoblotting; D045342:Light-Harvesting Protein Complexes; D045322:Photosynthetic Reaction Center Complex Proteins / Q000737:chemistry*; D045332:Photosystem II Protein Complex
D001426:Bacterial Proteins; D003432:Cross-Linking Reagents; D003573:Cytochrome b Group; D045342:Light-Harvesting Protein Complexes; D045322:Photosynthetic Reaction Center Complex Proteins; D045332:Photosystem II Protein Complex; C070970:photosystem II, chlorophyll-binding protein, CP-47; C019313:cytochrome b559