Regulation of matrix metabolism in equine cartilage explant cultures by interleukin 1.
Explant cultures were set up, using articular cartilage obtained from metatarsophalangeal joints of 11 horses. Explants from 2 horses were used to determine culture conditions appropriate for tissue viability. The cartilage explants maintained steady-state metabolism of proteoglycans during a 13-day evaluation period. The metabolic response of equine articular cartilage to incubation with recombinant human interleukin 1 (0.01 to 100 ng/ml) was studied, using cartilage obtained from the remaining 9 horses, age of which ranged from 3 months to 20 years. Interleukin 1 induced a dose-dependent release of glycosaminoglycan from the matrix during a 3-day incubation period. It also caused dose-dependent inhibition of glycosaminoglycan synthesis during a 3-hour pulse-labeling period. Explants obtained from older horses were significantly (P < 0.05) less responsive to interleukin 1, with respect to synthesis and release of glycosaminoglycan.
MacDonald
M H
MH
Department of Veterinary Anatomy, University of California, Davis 95616.
Stover
S M
SM
Willits
N H
NH
Benton
H P
HP
D000818:Animals; D002358:Cartilage, Articular / Q000378:metabolism*; D046508:Culture Techniques; D005109:Extracellular Matrix / Q000378:metabolism*; D005260:Female; D006025:Glycosaminoglycans / Q000096:biosynthesis / Q000378:metabolism*; D006736:Horses / Q000378:metabolism*; D007375:Interleukin-1 / Q000502:physiology*; D008297:Male
D006025:Glycosaminoglycans; D007375:Interleukin-1