title: Interleukin-1: the principal osteolytic cytokine produced by keratocysts.
abstract: Fragments of keratocysts removed at operation were maintained in explant culture and the media were assayed for the biological activity of the potent osteolytic cytokines--interleukin (IL)-1, interleukin (IL)-6 and tumour necrosis factor (TNF). Media were also assayed for their ability to stimulate bone resorption. All six cysts examined released IL-1 and IL-6 bioactivity but TNF bioactivity was unmeasurable. Dialysed cyst media stimulated bone resorption and this could be completely inhibited by a monospecific antibody which neutralized IL-1 alpha and IL-1 beta. Immunohistochemical staining of cryostat sections of keratocysts revealed the presence of IL-1 alpha and IL-6 in cyst epithelial cells but not in other cell types. Sections did not react with antibodies to IL-1 beta or TNF. It is therefore proposed that IL-1 alpha is the major osteolytic cytokine produced by keratocysts and that IL-6 and IL-1 may contribute to keratocyst growth by promoting epithelial cell proliferation and bone resorption, respectively.
authors: Joint Department of Maxillofacial Surgery and Oral Medicine, Eastman Dental Hospital, London, U.K.
authors: S
authors: S
authors: Meghji
authors: B
authors: B
authors: Henderson
authors: Y
authors: Y
authors: Bando
authors: M
authors: M
authors: Harris
mesh_terms: D000328:Adult; D000818:Animals; D001862:Bone Resorption; D002356:Cartilage / Q000201:enzymology; D017364:Collagenases / Q000032:analysis; D005881:Gingiva / Q000737:chemistry; D005882:Gingival Diseases / Q000201:enzymology / Q000378:metabolism / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D007375:Interleukin-1 / Q000032:analysis* / Q000494:pharmacology; D015850:Interleukin-6 / Q000032:analysis; D008213:Lymphocyte Activation; D051379:Mice; D009807:Odontogenic Cysts / Q000737:chemistry* / Q000201:enzymology / Q000473:pathology; D011817:Rabbits; D013601:T-Lymphocytes / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis
chemical_list: D007375:Interleukin-1; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D017364:Collagenases