Immunization of SCID-Hu mice and generation of anti-hepatitis B surface antigen-specific hybridomas by electrofusion.
Human hybridomas with specificity for recombinant hepatitis B surface antigen (HBSAg) were produced by adoptive transfer of human peripheral blood mononuclear cells prepared from HBSAg-immune donors to CB.17 mice bearing the severe combined immunodeficiency (SCID) phenotype. A total of ten SCID-Hu mice were immunized with recombinant HBSAg. Eight SCID-Hu mice found to have human HBSAg antibody in their serum were sacrificed, and single-cell suspensions were made from their spleens. The SCID-Hu spleen cells were electrofused to the mouse-human fusion partner H7. HBSAg-specific hybridomas were recovered from all fusions. This method may provide the means for the production of other human hybridomas and may, in some cases, circumvent the need for in vitro immunization or Epstein-Barr virus transformation of human B lymphocytes.
Neil
G A
GA
Department of Internal Medicine, University of Iowa, Iowa City 52242.
Sammons
D W
DW
D000328:Adult; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D002459:Cell Fusion; D004560:Electricity; D006510:Hepatitis B Antibodies / Q000096:biosynthesis*; D006801:Humans; D006825:Hybridomas / Q000276:immunology*; D007114:Immunization; D051379:Mice; D016513:Mice, SCID; D008875:Middle Aged
D000911:Antibodies, Monoclonal; D006510:Hepatitis B Antibodies