A new ELISA assay for islet cell surface antibodies determination in type 1 diabetes mellitus of recent onset.
Sera from 46 newly diagnosed type 1 diabetic patients and from 100 normal controls were screened for ICA Cf ICA (standard immunofluorescence test) and for ICSA (ELISA assay) detection. Among 46 patients, 33 (71.7%) were ICA positive (16 of them also Cf ICA positive) and 21 resulted ICSA positive (45%). No correlation was found between ICSA, ICA and Cf ICA respectively. Therefore the ICSA detection, by a new and sensitive ELISA assay, in addition to ICA immunofluorescence determination, may be of significant diagnostic value in IDDM of recent onset.
Bizzarro
A
A
Cattedra di Patologia Speciale Medica e Metodologia Clinica 3, Naples, Italy.
De Bellis
A
A
Florio
A
A
Gentile
S
S
Amoresano Paglionico
V
V
Cacciatore
F
F
Iacono
G
G
D000293:Adolescent; D000906:Antibodies / Q000032:analysis*; D002462:Cell Membrane / Q000276:immunology; D002648:Child; D002675:Child, Preschool; D003922:Diabetes Mellitus, Type 1 / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007515:Islets of Langerhans / Q000276:immunology*; D008297:Male; D013997:Time Factors
D000906:Antibodies