Determination of fatty acids in fish oil dietary supplements by capillary chromatography with laser-induced fluorescence detection.
The 4-bromomethyl-7-methoxycoumarin derivatives of 14 saturated and unsaturated fatty acids, including the omega-3 fatty acids, were separated by reversed-phase liquid chromatography and detected by laser-induced fluorescence. Baseline resolution was obtained by using a high-efficiency packed capillary column with 240,000 theoretical plates, together with a systematic optimization of the mobile phase composition. The retention indices of the fatty acid derivatives correlated well with a predictive empirical model, showing accuracy better than 0.46% relative error and reproducibility better than +/- 0.1% relative standard deviation. The physiologically important fatty acids with 12-22 carbon atoms and 0-6 double bonds were determined at the femtomole level in fish oil dietary supplements by using this methodology.
Yoo
J S
JS
Department of Chemistry, Michigan State University, East Lansing 48824.
McGuffin
V L
VL
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004042:Dietary Fats, Unsaturated / Q000032:analysis*; D005227:Fatty Acids / Q000032:analysis* / Q000737:chemistry; D005231:Fatty Acids, Unsaturated / Q000032:analysis / Q000737:chemistry; D005395:Fish Oils / Q000008:administration & dosage / Q000032:analysis*; D005453:Fluorescence; D007834:Lasers
D004042:Dietary Fats, Unsaturated; D005227:Fatty Acids; D005231:Fatty Acids, Unsaturated; D005395:Fish Oils