title: Ganglioside treatment partially counteracts neurotoxic effects of trimethyltin but may itself cause neurotoxicity in rats: experimental results and a critical review.
abstract: We have demonstrated a deficit in working memory and/or consolidation of information in working memory into reference memory by a single oral dose of the neurotoxin trimethyltin(TMT). Moreover, TMT causes loss of hippocampal corticosterone receptors and increases brain glial fibrillary acidic protein(GFAP), an index of the astrocytic reaction to diverse types of CNS lesions. We tried to block the TMT-induced cognitive deficit and these biochemical markers by treating rats with purified mixed gangliosides (GS) for 21 days, starting 2 days before the TMT treatment. As expected, TMT decreased the number of corticosterone receptors in hippocampi and increased the GFAP concentration in hippocampi and to a lesser extent, in frontal cortices, measured more than 8 mon after treatment. The small increase in GFAP in frontal cortices was attenuated by GS but not in hippocampi. The pronounced learning deficits caused by TMT were attenuated to a small extent by GS in the TMT-GS group, when a learning criterion was used for the last session's performance of acquired lever-directed behavior. GS also delayed the appearance of significant performance differences between Controls and TMT-treated rats, when probed with a progressive fixed ratio schedule of reinforcement. However, most measures of learning and performance indicated that GS did not block the dysfunctional consequences of TMT treatment but instead caused similar functional decrements in rats treated with water instead of TMT. Corticosterone receptors in hippocampi were reduced to about 65% of Controls in the TMT-Water, TMT-GS, and Water-GS groups. A reduction in corticosterone receptors in hippocampi after TMT treatment probably reflects the loss of one or more cell types (e.g., pyramidal cells), which is supported by the increase in GFAP in this region. However, we did not observe a reciprocal relation between steroid receptors and GFAP after GS alone, indicating that GS did not cause detectable cell loss or cell damage, measured in this manner. Thus, reactive gliosis probably was not a pre-condition for the cognitive dysfunction. The fact that the cognitive deficits are probably related to hippocampal dysfunction supports the notion of a causal relationship between corticosterone receptor reduction and/or their altered function and cognitive impairment of this special type. The possibility that our results demonstrate potential neurobehavioral toxicity of GS is discussed in light of many reports which present data that can be similarly interpreted.
authors: Department of Pharmacology, University of Minnesota, Minneapolis 55455.
authors: S B
authors: SB
authors: Sparber
authors: J P
authors: JP
authors: O'Callaghan
authors: B
authors: B
authors: Berra
mesh_terms: D000818:Animals; D001921:Brain / Q000033:anatomy & histology / Q000187:drug effects*; D003216:Conditioning, Operant / Q000187:drug effects*; D003345:Corticosterone; D005732:Gangliosides / Q000008:administration & dosage / Q000494:pharmacology* / Q000633:toxicity; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D006185:Habituation, Psychophysiologic / Q000187:drug effects; D006624:Hippocampus / Q000378:metabolism; D015918:Infusion Pumps, Implantable; D008297:Male; D009020:Morphine / Q000494:pharmacology; D009419:Nerve Tissue Proteins / Q000032:analysis / Q000187:drug effects; D009929:Organ Size / Q000187:drug effects; D051381:Rats; D011965:Receptors, Glucocorticoid / Q000187:drug effects; D011987:Receptors, Steroid; D012055:Reinforcement Schedule; D014298:Trimethyltin Compounds / Q000008:administration & dosage / Q000037:antagonists & inhibitors / Q000633:toxicity*
chemical_list: D005732:Gangliosides; D005904:Glial Fibrillary Acidic Protein; D009419:Nerve Tissue Proteins; D011965:Receptors, Glucocorticoid; D011987:Receptors, Steroid; D014298:Trimethyltin Compounds; C024357:corticosterone receptor; D009020:Morphine; D003345:Corticosterone