title: The effect of sulconazole on the antigenic composition of two dermatophyte species.
abstract: Trichophyton rubrum and Trichophyton interdigitale have been grown in liquid culture in the presence of sulconazole. The antigenic activity of detergent extracts of intact organisms was analysed following SDS-PAGE and the probing of Western blots with homologous antisera raised in rabbits and with sera from patients with dermatophyte infections. Differences in protein-band patterns were noted; some bands present in control samples were absent in azole-treated samples and vice versa. These differences were reflected in antigenic band patterns, especially among components of approximate molecular weight of 30-40, 50-60 and 92-100 kDa.
authors: Mycological Reference Laboratory, Central Public Health Laboratory, London, UK.
authors: V M
authors: VM
authors: Hearn
authors: M K
authors: MK
authors: Moore
authors: J P
authors: JP
authors: Latgé
authors: J N
authors: JN
authors: Fracchia
mesh_terms: D000818:Animals; D000935:Antifungal Agents / Q000494:pharmacology*; D000946:Antigens, Fungal / Q000032:analysis* / Q000187:drug effects; D003902:Detergents; D005656:Fungal Proteins / Q000032:analysis / Q000187:drug effects; D006023:Glycoproteins / Q000032:analysis / Q000187:drug effects; D006801:Humans; D007093:Imidazoles / Q000494:pharmacology*; D011817:Rabbits; D014249:Trichophyton / Q000187:drug effects* / Q000254:growth & development
chemical_list: D000935:Antifungal Agents; D000946:Antigens, Fungal; D003902:Detergents; D005656:Fungal Proteins; D006023:Glycoproteins; D007093:Imidazoles; C033308:sulconazole