Construction of a shuttle lacZ alpha-based Escherichia coli-actinomycetes vector containing the phage JHJ-3 replicon.
Using the broad replicating range JHJ-3 phage replicon, a shuttle vector for Escherichia coli and actinomycetes has been constructed. The vector, pOJ31, bears the lacZ alpha fragment allowing a blue/white gene cloning system. pOJ31 also contains a polylinker of 15 unique cloning sites and the phage T7 promoter. The vector has been used to stably express the mel gene from plasmid pIJ702 in Streptomyces lividans.
Gaudreau
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LR
Département de Biologie, Université de Sherbrooke, Québec, Canada.
Le Gouill
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C
Déry
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CV
D000192:Actinomycetales / Q000235:genetics*; D001435:Bacteriophages / Q000235:genetics; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D005822:Genetic Vectors; D007763:Lac Operon; D010957:Plasmids; D012093:Replicon; D013302:Streptomyces / Q000235:genetics