Synthesis of lipopolysaccharide by Escherichia coli cells recovering from sublethal heat stress.
Escherichia coli cells exposed to a sublethal heat treatment at 55 degrees C for 15s synthesized lipopolysaccharide during their recovery period after heat stress. As chloramphenicol at least partly inhibited the synthesis of lipopolysaccharide, it is suggested that its synthesis might be in part due to the lipopolysaccharide-synthesizing enzymes produced de novo. The results obtained coincided with our previous finding that the permeability barrier function was repaired by heat-stressed cells.
Tsuchido
T
T
Department of Biotechnology, Kansai University, Japan.
Takeuchi
A
A
Takano
M
M
D000220:Adaptation, Biological; D001426:Bacterial Proteins / Q000096:biosynthesis; D002463:Cell Membrane Permeability; D002701:Chloramphenicol / Q000494:pharmacology; D003470:Culture Media; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D005690:Galactose / Q000378:metabolism; D006358:Hot Temperature / Q000009:adverse effects*; D007930:Leucine / Q000378:metabolism; D008070:Lipopolysaccharides / Q000378:metabolism*
D001426:Bacterial Proteins; D003470:Culture Media; D008070:Lipopolysaccharides; D002701:Chloramphenicol; D007930:Leucine; D005690:Galactose