[A stereoscopic analysis of the centrosome structure in tissue culture cells under the action of carbonyl cyanide p-trifluoromethoxyphenylhydrazone and ouabain].
The action of carbonyl cyanide p-trifluoromethoxyphenylhydrazone (FCCP) and ouabain results in significant increase of the quantity of microtubules with attached and free proximal end around the centrosome. The majority of free microtubules are oriented with their proximal ends towards the heads of pericentriolar satellites or towards the walls of centriolar cylinders. The increasing of total number of microtubules is the result of the increasing of microtubules attached to or oriented towards the pericentriolar satellites. Comparing the action of FCCP and ouabain from one side and taxol from the other side it is possible to conclude that FCCP and ouabain promote the initiation of microtubule growth in the centrosome of they have an influence on the frequency of separation of the microtubules from microtubule nucleating centers.
Alieva
I B
IB
Vorob'eva
I A
IA
D000818:Animals; D002259:Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone / Q000494:pharmacology*; D002502:Centrioles / Q000187:drug effects / Q000648:ultrastructure; D046508:Culture Techniques; D003584:Cytological Techniques; D003593:Cytoplasm / Q000187:drug effects* / Q000648:ultrastructure; D008854:Microscopy, Electron; D008870:Microtubules / Q000187:drug effects / Q000648:ultrastructure; D010042:Ouabain / Q000494:pharmacology*; D013552:Swine; D013997:Time Factors
D002259:Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone; D010042:Ouabain
Stereoskopicheskiĭ analiz stroeniia tsentrosomy v kletkakh kul'tury tkani pri deĭstvii triftormetoksikarboniltsianidfenilgidrazona i uabaina.