Purification and amino acid sequence of sakacin A, a bacteriocin from Lactobacillus sake Lb706.
Sakacin A, a bacteriocin produced by Lactobacillus sake Lb706 and which inhibits the growth of Listeria monocytogenes, was purified to homogeneity by ammonium sulphate precipitation and ion-exchange, hydrophobic-interaction and reversed-phase chromatography. The complete amino acid sequence of sakacin A was determined by Edman degradation. The bacteriocin consisted of 41 amino acid residues and had a calculated M(r) of 4308.7, which is in good agreement with the value determined by mass spectrometry. The structural gene encoding sakacin A (sakA) was cloned and sequenced. The gene encoded a primary translation product of 59 amino acid residues which was cleaved between amino acids 18 and 19 to yield the active sakacin A. Sakacin A shared some sequence similarities with other bacteriocins.
Holck
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A
MATFORSK, Norwegian Food Research Institute, As.
Axelsson
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L
Birkeland
S E
SE
Aukrust
T
T
Blom
H
H
D000595:Amino Acid Sequence; D001430:Bacteriocins / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification* / Q000494:pharmacology; D001483:Base Sequence; D003001:Cloning, Molecular; D005798:Genes, Bacterial; D007778:Lactobacillus / Q000187:drug effects / Q000235:genetics*; D008089:Listeria monocytogenes / Q000187:drug effects; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011498:Protein Precursors / Q000235:genetics; D021382:Protein Sorting Signals / Q000235:genetics; D017386:Sequence Homology, Amino Acid
D001430:Bacteriocins; D011498:Protein Precursors; D021382:Protein Sorting Signals; C071456:sakacin A