von Willebrand factor antigen: a radial immunodiffusion method evaluated and compared with an ELISA method.
A new commercial kit method for the quantification of von Willebrand factor antigen (vWFAg) by radial immunodiffusion was compared to an established ELISA technique. Major discrepancies were found between the two methods. The radial immunodiffusion method had poorer intra- and inter-assay coefficients of variation than the ELISA method, although there was adequate inter-method agreement when 100 plasma samples from controls and patients were compared. However, there was a great difference in values obtained for vWFAg in the reference sample supplied with the commercial kit and that obtained directly from the National Institute for Biological Standards and Controls. There were also differences in levels of significance when vWFAg was measured by the two techniques in different clinical groups, and standard deviations were larger when the kit method was used. It is suggested that on scientific and economic grounds, the commercial radial immunodiffusion kit does not offer a competitive advantage over the ELISA method.
Blann
A D
AD
Department of Surgery, University Hospital of South Manchester, England, UK.
D001324:Autoantigens / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D005779:Immunodiffusion / Q000379:methods; D011933:Reagent Kits, Diagnostic; D014841:von Willebrand Factor / Q000276:immunology*
D001324:Autoantigens; D011933:Reagent Kits, Diagnostic; D014841:von Willebrand Factor