Confocal analysis of fluorescent bead uptake by mouse Peyer's patch follicle-associated M cells.
Latex beads coated with secretory immunoglobulin (IgA) instilled into mouse intestinal loops are taken up by M cells present in Peyer's patch follicle-associated epithelial tissue. Bead adsorption and uptake is greater at the edge compared with the apex of follicle domes. Coating beads with bovine serum albumin (BSA) causes a fourfold reduction in adsorption and a twentyfold reduction in uptake. Results demonstrate selectivity between adsorption and uptake and between the ability of different proteins to facilitate uptake.
Porta
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C
Dipartimento di Fisiologia e Biochimica Generali, Universita degli Studi di Milano, Italy.
James
P S
PS
Phillips
A D
AD
Savidge
T C
TC
Smith
M W
MW
Cremaschi
D
D
D000327:Adsorption; D000818:Animals; D005453:Fluorescence; D007071:Immunoglobulin A, Secretory / Q000378:metabolism; D007408:Intestinal Absorption; D007413:Intestinal Mucosa / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D010581:Peyer's Patches / Q000166:cytology / Q000378:metabolism*
D007071:Immunoglobulin A, Secretory