Detection of Listeria monocytogenes by non-radioactive RNA probe and polymerase chain reaction.
A HindIII fragment, 651-base-pair, from the listeriolysin 0 gene of Listeria monocytogenes was cloned into pGEM4Z. Radioactive 32P labeled and non-radioactive digoxigenin labeled RNA probes were made by in vitro transcriptions. These probes hybridized only to L. monocytogenes strains. Polymerase chain reaction was performed by using a 22-mer and a 23-mer oligonucleotide primer, and segment of about 700-base-pair was amplified only in L. monocytogenes strains and this band was visualized by hybridization with the non-radioactive probe.
Wong
H C
HC
Department of Microbiology, Soochow University, Taipei, Taiwan, R.O.C.
Chao
W L
WL
Yu
C M
CM
D001483:Base Sequence; D008089:Listeria monocytogenes / Q000235:genetics / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D015347:RNA Probes
D015347:RNA Probes