Biological assay for tissue kallikrein: comparison with the synthetic substrate S2266.
A highly sensitive biological assay for tissue kallikrein is described, using human kininogen as substrate; and quantitation, by radioimmunoassay, of generated kinins. Using purified human urinary kallikrein as a reference standard we have correlated the kininogenase activity of kallikrein with amidase activity as measured by cleavage of the synthetic substrate S2266.
Campbell
S K
SK
Department of Renal and Endocrine Medicine, University of Southampton, St. Mary's Hospital, Portsmouth, UK.
Marshall
K G
KG
Millar
J G
JG
Raman
G V
GV
Albano
J D
JD
D000818:Animals; D001681:Biological Assay / Q000379:methods* / Q000706:statistics & numerical data; D002863:Chromogenic Compounds; D006801:Humans; D066298:In Vitro Techniques; D007610:Kallikreins / Q000378:metabolism / Q000592:standards / Q000652:urine*; D007672:Kidney Cortex / Q000201:enzymology; D007705:Kinins / Q000032:analysis; D009842:Oligopeptides; D011863:Radioimmunoassay / Q000379:methods; D051381:Rats; D012015:Reference Standards; D012680:Sensitivity and Specificity; D013379:Substrate Specificity
D002863:Chromogenic Compounds; D007705:Kinins; D009842:Oligopeptides; C031006:Val-Leu-Arg-p-nitroanilide; D007610:Kallikreins