title: Analysis of primary bone formation in porous alumina: a fluorescence and scanning electron microscopic study of marrow cell induced osteogenesis.
abstract: Porous alumina ceramics alone and combined with rat marrow cells were implanted subcutaneously in the back of syngeneic Fischer rats. Fluorochrome-labeling was performed post operatively and the ceramics were harvested 6 and 8 weeks after implantation. Undecalcified sections of the implants were observed under fluorescence microscopy and the de novo bone-ceramic interfacial areas were analyzed by a scanning electron microscope equipped with an electron probe microanalyzer. Alumina ceramics alone did not show any bone formation, while all marrow cell loaded ceramics showed new bone formation 6 and 8 weeks after implantation. Bone formation was first observed in the center of the pores and proceeded in a centrifugal direction, leading to contact with the ceramic. These results suggest that bone marrow cells have inherent osteogenic capacity and in the pore region of alumina ceramics progression of the osteogenesis causes the dissipation of intervening fibrous tissue between the de novo bone and alumina ceramic surface.
authors: Department of Orthopedic Surgery, Nara Medical University, Japan.
authors: M
authors: M
authors: Okumura
authors: H
authors: H
authors: Ohgushi
authors: Y
authors: Y
authors: Takakura
authors: C A
authors: CA
authors: van Blitterswijk
authors: H K
authors: HK
authors: Koerten
mesh_terms: D000537:Aluminum Oxide / Q000737:chemistry*; D000818:Animals; D016026:Bone Marrow Transplantation / Q000502:physiology*; D002454:Cell Differentiation; D005069:Evaluation Studies as Topic; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D010012:Osteogenesis / Q000502:physiology*; D016062:Porosity; D051381:Rats; D011916:Rats, Inbred F344
chemical_list: D000537:Aluminum Oxide