Ultrastructure and histochemistry of the lymph system and parenchyma of juvenile paramphistomes (Paramphistomidae: Digenea) during migration in Indian ruminants.
The lymph system of juvenile Paramphistomum epiclitum and Fischoederius elongatus consists of a single pair of longitudinal primary vessels from which sub-dividing branches extend laterally to associate with most major tissues and organs. The system originates shortly after excystation in the definitive host and is fully developed in day 14 juveniles. Lymph vessels are syncytial and membrane limited, with a matrix which contains autophagic-like inclusions, clusters of SER and free nuclei. Similar organelles are evident in the matrix of parenchyma and specialized cells juxta-posing the pharynx (JP cells). These tissues are intimately associated and perhaps functionally integrated. Parenchyma represents a major site for carbohydrate storage and turnover, whilst the lymph appears to perform a similar role for proteins. The JP cells of juveniles display prolific autophagic-like activity only during migration, which coincides with the depletion of carbohydrate reserves in parenchyma. Key mitochondrial enzymes were histochemically demonstrated in the lymph despite the apparent absence of mitochondria from this system in post-day 14 juveniles. Succinate dehydrogenase activity was cytolocalized in mitochondria, whilst attempts to perform a similar localization of this enzyme in lymph were unsuccessful. The possibility of non-enzymatic interference in the histochemical demonstration of dehydrogenase is examined.
Mattison
R G
RG
School of Biology and Biochemistry, Queen's University of Belfast, Northern Ireland, U.K.
Hanna
R E
RE
Nizami
W A
WA
D000818:Animals; D002020:Buffaloes / Q000469:parasitology*; D015511:Goat Diseases / Q000469:parasitology*; D006041:Goats; D006651:Histocytochemistry; D008208:Lymphatic System / Q000648:ultrastructure; D008854:Microscopy, Electron; D010250:Paramphistomatidae / Q000648:ultrastructure*; D014201:Trematode Infections / Q000469:parasitology / Q000662:veterinary*