Measurement of ascorbic acid in platelets and its relationship to polymorphonuclear leukocyte levels.
A simplified method for measuring ascorbic acid (AA) concentrations in platelets is described. Platelet ascorbate was determined as the difference in concentrations between platelet rich plasma and platelet free plasma. In 20 healthy individuals concentrations of AA in platelets and polymorphonuclear leukocytes were found to be significantly correlated (r = 0.72, P < 0.01). Measurement of platelet ascorbate may provide an estimate of tissue AA status.
McCulloch
R K
RK
Department of Medicine, University of Western Australia, Royal Perth Hospital.
Vandongen
R
R
D000328:Adult; D001205:Ascorbic Acid / Q000097:blood*; D001792:Blood Platelets / Q000737:chemistry / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D004912:Erythrocytes / Q000737:chemistry / Q000378:metabolism; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000737:chemistry / Q000378:metabolism*
D001205:Ascorbic Acid