The requirements for activation of an antigen specific T-cell clone via the CD3 complex.
We have studied the requirements for the activation of the haemagglutinin peptide specific cloned T-cell HA1.7 by anti-CD3 antibodies and the mechanism of modulation of the CD3 complex. Cross-linking of the complex either by a soluble antibody second layer or an immobilized matrix of antibody is necessary for induction of a proliferative response. This proliferative response is totally dependent on exogenously added IL-2. Modulation of the cross-linked CD3 complex is by patching, capping and internalization of the antigen and is not essential for the proliferative response.
Colaco
C A
CA
Quadrant Research Foundation, Cambridge, Great Britain.
Lamb
J R
JR
D000818:Animals; D000906:Antibodies / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology; D000938:Antigen-Presenting Cells / Q000276:immunology*; D017252:CD3 Complex / Q000276:immunology*; D002999:Clone Cells; D004705:Endocytosis; D006680:HLA Antigens / Q000276:immunology; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology; D008213:Lymphocyte Activation; D051379:Mice; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology*
D000906:Antibodies; D000911:Antibodies, Monoclonal; D017252:CD3 Complex; D006680:HLA Antigens; D007376:Interleukin-2