Urinary testosterone measurement by gas chromatography after solid-phase extraction and high-performance liquid chromatography.
A method was developed for the rapid determination of testosterone in urine. The procedure consists of solid-phase extraction (SPE) followed by high-performance liquid chromatographic (HPLC) clean-up before gas chromatographic determination. Recovery was evaluated by adding [3H]testosterone (10(4) cpm) to urine samples; the mean recovery of radioactivity after SPE and HPLC was 82%. Precision was estimated by repeated measurement of testosterone in four different urine samples; the coefficient of variation was 7.9% (95% confidence limits 6.1-11.4%). Accuracy was evaluated by standard addition and dilution assays; a linear relationship was found between the expected and observed values (r2 = 0.982). The method is rapid, effective and suitable for routine analysis.
Venturelli
E
E
National Cancer Institute, Endocrine Unit, Milan, Italy.
Manzari
A
A
Cavalleri
A
A
Benzo
M
M
Secreto
G
G
Marubini
E
E
D002849:Chromatography, Gas / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D013739:Testosterone / Q000652:urine*; D014316:Tritium
D014316:Tritium; D013739:Testosterone