Isolation and identification of C-type natriuretic peptide in human monocytic cell line, THP-1.
In a survey for unknown bioactive peptides in mammalian cell lines, we isolated peptides exhibiting a strong relaxant effect on chick rectum from a phorbol ester-supplemented culture medium of the human monocytic cell line, THP-1. The peptide was deduced to be a C-terminal 29-residue peptide derived from a human C-type natriuretic peptide (CNP) precursor. CNP mRNA was also detected in the THP-1 cells, and expression of CNP gene and CNP concentration in the culture medium was found to be highly augmented by the stimulation of phorbol ester. Production of CNP in THP-1 cells suggests that CNP also functions as a local regulator in the blood cell-vascular system, although CNP has previously been recognized as a neuropeptide functioning in the central nervous system.
Ishizaka
Y
Y
Department of Biochemistry, Miyazaki Medical College, Japan.
Kangawa
K
K
Minamino
N
N
Ishii
K
K
Takano
S
S
Eto
T
T
Matsuo
H
H
D000595:Amino Acid Sequence; D000818:Animals; D009320:Atrial Natriuretic Factor / Q000235:genetics / Q000302:isolation & purification* / Q000494:pharmacology; D002645:Chickens; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D066298:In Vitro Techniques; D007948:Leukemia, Monocytic, Acute; D008969:Molecular Sequence Data; D009126:Muscle Relaxation / Q000187:drug effects; D009130:Muscle, Smooth / Q000187:drug effects / Q000502:physiology*; D011498:Protein Precursors / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D012007:Rectum / Q000187:drug effects / Q000502:physiology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D011498:Protein Precursors; D012333:RNA, Messenger; D009320:Atrial Natriuretic Factor; D013755:Tetradecanoylphorbol Acetate