[Immunohistochemistry of bovine lymphoid tissue: neutralization of the non-specific reactivities of serum anti-immunoglobulins by bovine serum].
In lymphoid tissue, the presence of high amounts of free proteins and immunoglobulins frequently leads to the observation of non-specific reactivities during immunohistochemical reactions. The types of anti-immunoglobulin sera utilized in these immunohistochemical reactions may be capable of identifying immunoglobulins secreted or harboured by the tissue cells under study, leading to non-specific marking. Incubation of these antisera with serum from the species in which the lymphoid tissue is studied neutralizes the non-specific reactivities connected with the presence of tissue immunoglobulins.
Delverdier
M
M
Ecole nationale vétérinaire, laboratoire associé INRA, Toulouse, France.
Pradeau
P
P
Abella
N
N
Schelcher
F
F
Espinasse
J
J
Cabanié
P
P
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology; D000918:Antibody Specificity; D002021:Buffers; D002417:Cattle / Q000033:anatomy & histology* / Q000276:immunology; D005260:Female; D007106:Immune Sera / Q000276:immunology; D007136:Immunoglobulins / Q000032:analysis* / Q000276:immunology; D007150:Immunohistochemistry; D008198:Lymph Nodes / Q000276:immunology; D008221:Lymphoid Tissue / Q000276:immunology*; D008264:Macrophages / Q000276:immunology; D051379:Mice; D011817:Rabbits; D013552:Swine
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D002021:Buffers; D007106:Immune Sera; D007136:Immunoglobulins
Immunohistochimie du tissu lymphoïde des bovins: neutralisation des réactivités non spécifiques des sérums anti-immunoglobulines d'espèce par du sérum bovin.