Detection of T-lymphocytes in the peripheral blood and lymphoid tissues of cattle using a variant of the E-rosette test and monoclonal antibodies.
A simple and efficient variant of the E-rosette test based on addition of dextran to the incubation media is described. This variant 1) does not include B cells 2) involves some CD2+ null cells as described 3) is not inhibited by anti-CD5 antibody 4) correlates with CD2 expression 5) detects specific changes in the relative proportion of T-lymphocytes under the different conditions. In a group of calves the mean percentage of RFC in the peripheral blood lymphocytes was 53.59 +/- 8.70 and in cows there was 72.57 +/- 3.85. The proportion of RFC detected in bovine leukemia virus (BLV)--infected cows with lymphocytosis was less than one third of that in BLV--negative animals and vice versa in B (MHC class II+) lymphocytes.
Vojtísek
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University of Veterinary Science, Institute of Animal Breeding and Genetics, Brno, CSFR.
Knotek
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Horín
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Mádr
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D000818:Animals; D002417:Cattle / Q000097:blood / Q000276:immunology*; D016583:Enzootic Bovine Leukosis / Q000276:immunology*; D005260:Female; D008221:Lymphoid Tissue / Q000473:pathology*; D015203:Reproducibility of Results; D012397:Rosette Formation / Q000662:veterinary*; D012680:Sensitivity and Specificity; D013601:T-Lymphocytes