Evaluation of plant extracts for antileishmanial activity using a mechanism-based radiorespirometric microtechnique (RAM).
Extracts of eleven plants used in Nigerian traditional medicine have been evaluated for possible antileishmanial activity using a radiorespirometric microtest technique based on in vitro inhibition of catabolism of 14CO2 from a battery of 14C-substrates by promastigotes. Of 13 methanol extracts tested, 5 from Gongronema latifolia, Dorstenia multiradiata, Picralima nitida, Cola attiensis, and Desmodium gangeticum, were active at concentrations of 50 micrograms/ml or less against a visceral Leishmania isolate.
Iwu
M M
MM
Department of Medicinal Chemistry, Walter Reed Army Institute of Research, Washington, D.C. 20307.
Jackson
J E
JE
Tally
J D
JD
Klayman
D L
DL
D000818:Animals; D000981:Antiprotozoal Agents / Q000302:isolation & purification / Q000494:pharmacology*; D002245:Carbon Dioxide / Q000378:metabolism; D007891:Leishmania / Q000187:drug effects* / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry*
D000981:Antiprotozoal Agents; D010936:Plant Extracts; D002245:Carbon Dioxide