New monoclonal antibodies specific to HLA class I and their application in the study of acid treatment effect on cell surface antigens.
New monoclonal antibodies (mAbs) against HLA class I antigens were prepared and characterized. Three mAbs (CLI-1, CLI-2, CLI-3) were directed to the heavy chain of HLA class I molecules and one mAb (B2M-1) is specific to beta 2-microglobulin. Using these mAbs, it was confirmed that acid treatment selectively eliminated cell surface HLA class I antigenicity. The treatment with citric acid solution resulted in preparation of cells with lower residual HLA class I surface molecules than was seen after the treatment with glycine buffer.
Karpatová
M
M
Cancer Research Institute, Slovak Academy of Sciences, Bratislava.
Poláková
K
K
D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry*; D000918:Antibody Specificity; D000954:Antigens, Surface / Q000187:drug effects* / Q000276:immunology*; D002951:Citrates; D019343:Citric Acid; D005455:Fluorescent Antibody Technique; D015395:Histocompatibility Antigens Class I / Q000187:drug effects* / Q000276:immunology*; D006801:Humans; D006851:Hydrochloric Acid; D006863:Hydrogen-Ion Concentration; D051379:Mice; D008807:Mice, Inbred BALB C; D011233:Precipitin Tests; D012996:Solutions
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D002951:Citrates; D015395:Histocompatibility Antigens Class I; D012996:Solutions; D019343:Citric Acid; D006851:Hydrochloric Acid