In vitro stimulation of peripheral blood mononuclear cells by phytohaemagglutinin A induces CD45RA expression on monocytes.
Following phytohaemagglutinin A stimulation, CD45RA positive monocytes increased from 12.2 +/- 8.9% to 63.5 +/- 8.8% (p < 0.001), without a significant change in cell surface antigen density. In contrast, HLA-DQ antigen, also expressed in 76.8 +/- 10.5% of the monocytes after PHA stimulation for 48 h, revealed a marked enhancement in fluorescence intensity (p < 0.001). This up-regulation is already evident in unstimulated cultures. The percentages of CD45RA and HLA-DQ positive monocytes were correlated (r = 0.80, p < 0.001), substantiating a previous clinical observation. CD45RA expression may probe activated mono/macrophages.
Brohée
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Department of Internal Medicine, Université Libre de Bruxelles, C.H.U. André Vésale, Montigny-le-Tilleul, Belgium.
Higuet
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N
D000328:Adult; D000943:Antigens, Differentiation / Q000032:analysis; D002478:Cells, Cultured; D006683:HLA-DQ Antigens / Q000032:analysis; D006801:Humans; D017493:Leukocyte Common Antigens / Q000032:analysis / Q000096:biosynthesis*; D009000:Monocytes / Q000276:immunology*; D010835:Phytohemagglutinins / Q000494:pharmacology*; D012044:Regression Analysis
D000943:Antigens, Differentiation; D006683:HLA-DQ Antigens; D010835:Phytohemagglutinins; D017493:Leukocyte Common Antigens