title: Influence of bile acids on stimulated lipid peroxidation and hydrogen peroxide production in rat liver microsomes.
abstract: Bile acids were found to be effective antioxidants in bile and intestine. The influence of different bile acids on the NADPH-Fe(++)-stimulated lipid peroxidation (LPO) and cytochrome P-450 dependent hydrogen peroxide production (H2O2) in rat liver microsomes was investigated in vitro. LPO was determined as production of thiobarbituric acid reactants (TBAR). Different tri-, di- and monohydroxylated bile acids and cholesterol were given to the incubation mixture in concentrations ranging from 10(-5) to 10(-3) M. Sodium salts of cholic, tauroglycocholic and deoxycholic acids as well as cheno-deoxycholic, ursodeoxycholic, lithocholic acids and cholesterol did not alter the microsomal production of TBAR. H2O2 formation was significantly decreased by sodium deoxycholate whereas cholesterol increased H2O2 production up to 4 times. These results show that bile acids were not able to protect microsomal membrane lipids against peroxidative damage. Cholesterol mediated H2O2 formation as a source of hydroxyl radicals had no toxic effect concerning LPO, TBAR were not enhanced significantly.
authors: Institute of Pharmacology and Toxicology, Friedrich Schiller University Jena, Germany.
authors: A
authors: A
authors: Barth
authors: M
authors: M
authors: Bernst
mesh_terms: D000818:Animals; D001647:Bile Acids and Salts / Q000494:pharmacology*; D002251:Carbon Tetrachloride / Q000494:pharmacology; D002784:Cholesterol / Q000494:pharmacology; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D005296:Ferrous Compounds / Q000494:pharmacology; D006861:Hydrogen Peroxide / Q000378:metabolism*; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism*; D009249:NADP / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D013858:Thiobarbiturates
chemical_list: D001647:Bile Acids and Salts; D005296:Ferrous Compounds; D013858:Thiobarbiturates; D009249:NADP; D003577:Cytochrome P-450 Enzyme System; D002784:Cholesterol; D006861:Hydrogen Peroxide; D002251:Carbon Tetrachloride; C029684:thiobarbituric acid