Quantitative analysis of collagen and elastin cross-links using a single-column system.
The separation of both the immature and mature cross-links present in collagen together with the stable cross-links of elastin has been achieved on a single ion-exchange column. This technique avoids the current necessity for two different systems, ion-exchange and high-performance liquid chromatography-fluorescence techniques. The value of the method is illustrated by the comparison of the cross-link contents of aging bovine skin, from foetal to old age.
Sims
T J
TJ
Department of Veterinary Medicine, University of Bristol, Langford, UK.
Bailey
A J
AJ
D000375:Aging / Q000378:metabolism; D000818:Animals; D002417:Cattle; D002852:Chromatography, Ion Exchange / Q000379:methods*; D003094:Collagen / Q000032:analysis* / Q000378:metabolism; D003432:Cross-Linking Reagents / Q000378:metabolism; D004549:Elastin / Q000032:analysis* / Q000378:metabolism
D003432:Cross-Linking Reagents; D003094:Collagen; D004549:Elastin