title: Light microscopic localization of angiotensin II binding sites in canine medulla using high resolution autoradiography.
abstract: Angiotensin II (Ang II) produces dose-related, site-specific cardiovascular effects in the canine and rat dorsal medulla. Our previous studies suggested that Ang II binding sites are associated with presynaptic vagal afferent fibers in the nucleus tractus solitarii (nTS) and vagal efferent neurons in the dorsal motor nucleus of the vagus (dmnX). High resolution autoradiography now establishes the relationship of putative Ang II receptors to the cytoarchitecture of these nuclei. Sections of the canine medulla oblongata were processed for film or emulsion autoradiography with 0.3-1 nM 125I-Ang II. Quantitative densitometry of films before and after processing sections for emulsion coating confirmed no selective alteration in labeling. In emulsion coated sections, dense labeling was seen over the majority of the large perikarya and surrounding neuropil in the ventral dmnX. Bands of label overlaid vagal efferent fibers coursing ventrolaterally to exit the medulla. In the nTS, Ang II binding was restricted to regions with heavy vagal afferent innervation. In the dorsal nTS, label was distributed over both cell bodies and neuropil, with highest density capping the solitary tract. In the medial nTS, label was concentrated over perikarya, with scattered grains over the intervening neuropil. The discrete subnuclear association of Ang II binding sites in the dorsal medulla with vagal cells and fibers documents that Ang II receptors are present on both afferent vagal fibers and intrinsic medullary neurons, and reveals an anatomical substrate for the autonomic effects of Ang II in this region.
authors: Department of Brain, Cleveland Clinic Foundation, OH 44195-5286.
authors: E M
authors: EM
authors: Szigethy
authors: K L
authors: KL
authors: Barnes
authors: D I
authors: DI
authors: Diz
mesh_terms: D000804:Angiotensin II / Q000378:metabolism*; D000818:Animals; D001345:Autoradiography; D004285:Dogs; D007457:Iodine Radioisotopes; D008297:Male; D008526:Medulla Oblongata / Q000166:cytology / Q000378:metabolism*; D011945:Receptors, Angiotensin / Q000032:analysis / Q000378:metabolism*
chemical_list: D007457:Iodine Radioisotopes; D011945:Receptors, Angiotensin; D000804:Angiotensin II