High-performance liquid chromatography of human milk triacylglycerols and gas chromatography of component fatty acids.
Human milk triacylglycerols were separated by high-performance liquid chromatography. A 5-mu Supelcosil LC-18 column (Supelco, Inc., Bellefonte, PA) was used with acetone/acetonitrile (64:36, vol/vol) as mobile phase. Triacylglycerols were tentatively identified based on theoretical carbon number and relative retention time. Despite changes resulting from dietary fat variation, the major component triacylglycerols were those composed of palmitic, oleic and linoleic acids. Triacylglycerols with palmitic, stearic and oleic acids were present as minor components. Fatty acids were quantified by gas chromatography relative to an internal standard. Ratios of n-6/n-3 fatty acids were found to be higher than previously reported.
Dotson
K D
KD
Division of Nutritional Sciences, University of Illinois, Urbana-Champaign 61801.
Jerrell
J P
JP
Picciano
M F
MF
Perkins
E G
EG
D000273:Adipose Tissue / Q000737:chemistry; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D004041:Dietary Fats / Q000008:administration & dosage; D005227:Fatty Acids / Q000032:analysis*; D005260:Female; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000032:analysis; D008895:Milk, Human / Q000737:chemistry*; D019301:Oleic Acid; D009829:Oleic Acids / Q000032:analysis; D019308:Palmitic Acid; D010169:Palmitic Acids / Q000032:analysis; D013229:Stearic Acids / Q000032:analysis; D014280:Triglycerides / Q000032:analysis*
D004041:Dietary Fats; D005227:Fatty Acids; D008041:Linoleic Acids; D009829:Oleic Acids; D010169:Palmitic Acids; D013229:Stearic Acids; D014280:Triglycerides; D019301:Oleic Acid; D019308:Palmitic Acid; C031183:stearic acid; D019787:Linoleic Acid