[The effect of intracellular concentrations of tRNA, corresponding to the rare arginine codons AGG and AGA, on the gene expression in Escherichia coli].
Influence of increased arginine concentrations of tRNA's corresponding to rare codons AGG and AGA was studied in the model system constructed earlier. The model system is a chimeric gene consisting of CAT gene fragment, part of the gene encoding for alpha-domain of beta-galactosidase E. coli and a series of synthetic inserts enriched with codons AGG and AGA. In order to increase the intracellular tRNA concentration the natural gene of AGA-specific tRNA and the artificial gene of AGG-specific tRNA were cloned in plasmid under the control of p15A ori compatible with co1EI ori and used for maintaining the model gene. It was shown that the artificial AGG-specific tRNA gene produces a functionally active tRNA. A steep rise in the synthesis of polypeptide encoded by the model template containing rare codons was demonstrated when the genes of tRNAs recognizing these codons were propagated in the multicopy plasmid. It was shown that AGA-specific tRNA efficiently translates both AGA and AGG codons while AGG-specific tRNA - only AGG codons.
Gurskiĭ
Ia G
IaG
Marimont
N Iu
NIu
Bibilashvili
R Sh
RSh
D001120:Arginine / Q000235:genetics*; D001483:Base Sequence; D002678:Chimera; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D003062:Codon; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics*; D015870:Gene Expression; D008969:Molecular Sequence Data; D010957:Plasmids; D012343:RNA, Transfer / Q000378:metabolism*; D001616:beta-Galactosidase / Q000235:genetics
D003062:Codon; D012343:RNA, Transfer; D001120:Arginine; D015500:Chloramphenicol O-Acetyltransferase; D001616:beta-Galactosidase
Vliianie vnutrikletochnykh kontsentratsiĭ tRNK, sootvetstvuiushchikh redkim argininovym kodonam AGG i AGA, na ékspressiiu genov v Escherichia coli.