Kinetics and organ distribution of [14C]-sialic acid-GM3 and [3H]-sphingosine-GM1 after intravenous injection in rats.
Serum kinetics and organ distribution of [14C]-sialic acid-GM3 and [3H]-sphingosine-GM1, administered as an intravenous bolus, were analysed in Wistar rats. [3H]-GM1 and [14C]-GM3 had serum half-lives of 1.4 hours and 1.8 hours, respectively. Three hours after injection 75% of the GM1- and 38% of the GM3-associated label were present in the liver. Smaller yet significant amounts of label were present in the central nervous system, kidneys and lung. In vitro studies showed that [14C]-GM3 and [3H]-GM1 incubated with serum were predominantly bound to the High Density Lipoprotein and the Low Density Lipoprotein fractions. These results suggest a rapid serum clearance of exogenous gangliosides by the liver in rats.
Dumontet
C
C
Unité INSERM 218, Centre Léon Bérard, Lyon, France.
Rebbaa
A
A
Portoukalian
J
J
D000818:Animals; D001921:Brain / Q000378:metabolism; D002250:Carbon Radioisotopes; D005260:Female; D005677:G(M1) Ganglioside / Q000097:blood / Q000493:pharmacokinetics*; D005679:G(M3) Ganglioside / Q000097:blood / Q000493:pharmacokinetics*; D006207:Half-Life; D006801:Humans; D007668:Kidney / Q000378:metabolism; D007700:Kinetics; D008074:Lipoproteins / Q000097:blood*; D008099:Liver / Q000378:metabolism; D008168:Lung / Q000378:metabolism; D019158:N-Acetylneuraminic Acid; D051381:Rats; D017208:Rats, Wistar; D012794:Sialic Acids / Q000378:metabolism; D013110:Sphingosine / Q000378:metabolism; D013997:Time Factors; D014018:Tissue Distribution; D014316:Tritium
D002250:Carbon Radioisotopes; D005679:G(M3) Ganglioside; D008074:Lipoproteins; D012794:Sialic Acids; D014316:Tritium; D005677:G(M1) Ganglioside; D019158:N-Acetylneuraminic Acid; D013110:Sphingosine