title: Species dependency of in vitro macrophage activation by bacterial peptidoglycans.
abstract: The effect of various bacterial cell wall components on in vitro biological function of murine peritoneal exudate macrophages was evaluated. We examined four different parameters of metabolic activity and monokine secretion. Peritoneal exudate macrophages from rats and guinea pigs, all of the strains tested, were stimulated by whole bacterial cell wall preparations, purified bacterial cell wall peptidoglucans, its water-soluble peptidolglycan fragments, muramyl dipeptides and amphipathic substances. Murine peritoneal exudate macrophages were activated by amphipathic substances of gram-positive bacteria. However, macrophages from mice, irrespective of strains, were not stimulated in the in vitro assay systems by purified bacterial cell wall peptidoglycan, water-soluble bacterial peptidoglycan fragments or muramyl dipeptides. These results suggest that macrophage activation by bacterial peptidoglycan in vitro is animal species specific.
authors: Department of Biochemistry, Shimane Medical University, Japan.
authors: S
authors: S
authors: Nagao
authors: K S
authors: KS
authors: Akagawa
authors: F
authors: F
authors: Okada
authors: Y
authors: Y
authors: Harada
authors: K
authors: K
authors: Yagawa
authors: K
authors: K
authors: Kato
authors: Y
authors: Y
authors: Tanigawa
mesh_terms: D000119:Acetylmuramyl-Alanyl-Isoglutamine / Q000031:analogs & derivatives / Q000494:pharmacology; D000818:Animals; D002473:Cell Wall / Q000737:chemistry / Q000276:immunology; D003602:Cytotoxicity, Immunologic; D005260:Female; D005944:Glucosamine / Q000378:metabolism; D006094:Gram-Positive Bacteria / Q000276:immunology* / Q000648:ultrastructure; D006168:Guinea Pigs; D066298:In Vitro Techniques; D007375:Interleukin-1 / Q000096:biosynthesis; D008070:Lipopolysaccharides / Q000494:pharmacology; D008262:Macrophage Activation / Q000187:drug effects* / Q000276:immunology; D008264:Macrophages / Q000187:drug effects / Q000276:immunology; D051379:Mice; D008815:Mice, Inbred Strains; D010457:Peptidoglycan / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D013045:Species Specificity; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
chemical_list: D007375:Interleukin-1; D008070:Lipopolysaccharides; D010457:Peptidoglycan; D014409:Tumor Necrosis Factor-alpha; D000119:Acetylmuramyl-Alanyl-Isoglutamine; D005944:Glucosamine