title: A new individualization marker of semen: deoxyribonuclease I (DNase I) polymorphism.
abstract: We describe a method for obtaining specific and reproducible deoxyribonuclease I (DNase I) typing from liquid semen. Isoelectric focusing of the enzymes on polyacrylamide gel (IEF-PAGE, pH 3.5-5) was accomplished using a 0.5-mm thick gel. The separated isozymes were visualized by a new activity staining method, dried agarose film-overlay (DAFO). Pretreatment of semen samples with neuraminidase markedly enhanced the isozyme-band resolution and sensitivity. The method was simple and reliable, with high resolution and sensitivity. The DNase I types in semen samples were correlated with the types found in corresponding blood and urine samples. DNase I typing could therefore provide an additional discriminant characteristic in the forensic examination of semen.
authors: Department of Legal Medicine, Fukui Medical School, Japan.
authors: K
authors: K
authors: Sawazaki
authors: T
authors: T
authors: Yasuda
authors: D
authors: D
authors: Nadano
authors: E
authors: E
authors: Tenjo
authors: R
authors: R
authors: Iida
authors: H
authors: H
authors: Takeshita
authors: K
authors: K
authors: Kishi
mesh_terms: D003850:Deoxyribonuclease I / Q000032:analysis*; D005554:Forensic Medicine / Q000379:methods*; D005819:Genetic Markers; D006801:Humans; D007525:Isoelectric Focusing; D008297:Male; D011110:Polymorphism, Genetic; D015203:Reproducibility of Results; D012661:Semen / Q000737:chemistry*
chemical_list: D005819:Genetic Markers; D003850:Deoxyribonuclease I