title: A red cell band 3 variant with altered stilbene disulphonate binding is associated with the Diego (Dia) blood group antigen.
abstract: 1. We have shown that the Dia antigen of the Diego blood group system is associated with the presence of red cell band 3 Memphis, but not all band 3 Memphis samples carry the Dia antigen. 2. The band 3 Memphis associated with the Dia antigen was covalently labelled by 4,4'-di-isothiocyanato-1,2-diphenylethane-2,2'-disulphonic acid (H2DIDS) more readily than was normal band 3 or band 3 Memphis not associated with the Dia antigen. This altered reactivity with H2DIDS has previously been noted for a band 3 Memphis sub-type designated variant 2. 3. This is the first example of a band 3 polymorphism associated with an antigenic change in the extracellular region of the band 3 polypeptide and with altered H2DIDS binding.
authors: Department of Immunochemistry, South Western Regional Transfusion Centre, Bristol, U.K.
authors: F A
authors: FA
authors: Spring
authors: L J
authors: LJ
authors: Bruce
authors: D J
authors: DJ
authors: Anstee
authors: M J
authors: MJ
authors: Tanner
mesh_terms: D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid / Q000031:analogs & derivatives* / Q000378:metabolism; D001457:Anion Exchange Protein 1, Erythrocyte / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D001789:Blood Group Antigens / Q000502:physiology*; D004912:Erythrocytes / Q000276:immunology / Q000378:metabolism; D005110:Extracellular Space / Q000378:metabolism; D014644:Genetic Variation / Q000502:physiology; D006801:Humans; D011110:Polymorphism, Genetic; D011485:Protein Binding
chemical_list: D001457:Anion Exchange Protein 1, Erythrocyte; D001789:Blood Group Antigens; C070316:band 3 protein Memphis; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid; D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid