Analysis of Acanthurus triostegus for marine toxins by the stick enzyme immunoassay and mouse bioassay.
The herbivorous convict tang or surgeon fish, Acanthurus triostegus, collected at different locations in the Hawaiian islands, was analyzed for toxicity using a mouse bioassay and a stick enzyme immunoassay (S-EIA) developed to detect ciguatoxin and closely related polyethers. Results of the S-EIA test indicated that about 94% of the samples gave negative readings, while 6% of the fish were positive and thus considered toxic. Fish samples from each location were pooled and the flesh and viscera were successively extracted with hexane, methanol, and water and tested in a mouse bioassay. About one-third of the methanol soluble fractions killed mice within 20 min, 70% killed within 4 hr, and 85% killed within 48 hr. About 40% of the hexane-soluble fractions killed mice within 24 hr and 55% killed within 48 hr. The water-soluble extracts of the flesh and viscera of fish taken from three locations showed relatively high toxicity. Common symptoms for all fractions included convulsions and jumping (especially just prior to death), respiratory distress, hind leg to complete paralysis, loss of body tone, and tremors. Analysis of the data suggests that in the flesh and viscera of A. triostegus there are at least three different nervous system-type toxins, most of which did not appear to react to the S-EIA test.
Iwaoka
W
W
Department of Food Science & Human Nutrition, University of Hawaii at Manoa, Honolulu 96822.
Horita
J
J
Shimojo
R
R
Tran
T
T
D000818:Animals; D001681:Biological Assay; D005260:Female; D005399:Fishes / Q000378:metabolism*; D006254:Hawaii; D007124:Immunoenzyme Techniques; D008387:Marine Toxins / Q000032:analysis* / Q000633:toxicity; D051379:Mice; D014020:Tissue Extracts / Q000032:analysis / Q000633:toxicity
D008387:Marine Toxins; D014020:Tissue Extracts