The retention behaviour of conjugated bile acids in reversed phase high performance liquid chromatography.
The retention behaviour of conjugated bile acids has been studied in a reversed phase high performance liquid chromatographic (RP-HPLC) system by using the mixture of methanol and aqueous phosphate buffer as the mobile phase. The retentions of the conjugates in RP-HPLC have been found to be mainly controlled by the glycine and taurine groups. The selectivity between five different glycine and taurine conjugated bile acids is a constant in RP-HPLC. This selectivity has been used for peak identification in the practical separation of conjugated bile acids.
Zhang
Y K
YK
National Chromatographic R. & A. Centre, Dalian Institute of Chemical Physics, Chinese Academy of Sciences.
Chen
N
N
Zao
R H
RH
Li
T
T
Wang
Z Y
ZY
Lu
P C
PC
D001646:Bile / Q000737:chemistry*; D001647:Bile Acids and Salts / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D001660:Biliary Tract Diseases / Q000378:metabolism; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005998:Glycine / Q000032:analysis / Q000378:metabolism; D006801:Humans; D008107:Liver Diseases / Q000378:metabolism; D000432:Methanol; D012044:Regression Analysis; D013654:Taurine / Q000032:analysis / Q000378:metabolism
D001647:Bile Acids and Salts; D013654:Taurine; D005998:Glycine; D000432:Methanol