On the blood-brain barrier to peptides: effects of immobilization stress on regional blood supply and accumulation of labelled peptides in the rat brain.
Tritiated arginine-vasopressin (AVP), desglycinamide-vasopressin (DGAVP), chicken gonadotropin releasing hormone (GnRH) or carbetocin were injected intracarotidally into rats exposed to a restraint stress for 60 min. The peptide accumulations were determined in 9-13 brain regions and anterior pituitary. In separate experiments the cerebral blood flow was measured. The blood supply to the brain was decreased in stressed animals as indicated by: 1. significant decrease (17-50%) of cerebral blood flow; 2. diminished accumulation of tritiated AVP in the regions lacking a blood-brain barrier (BBB). Consequently, the values of peptide accumulation were corrected for the changed blood supply. Compared with control animals, restraint stress induced a higher accumulation of AVP (+41%), DGAVP (+60%), carbetocin (+81%) and GnRH (+104%).
Dvorská
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I
Institute of Organic Chemistry and Biochemistry, Czechoslovak Academy of Sciences, Praha.
Brust
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P
Hrbas
P
P
Rühle
H J
HJ
Barth
T
T
Ermisch
A
A
D000818:Animals; D001812:Blood-Brain Barrier / Q000187:drug effects / Q000502:physiology*; D001921:Brain / Q000098:blood supply* / Q000378:metabolism*; D001923:Brain Chemistry; D007103:Immobilization / Q000502:physiology*; D008297:Male; D010455:Peptides / Q000378:metabolism*; D051381:Rats; D012039:Regional Blood Flow; D013312:Stress, Physiological / Q000378:metabolism* / Q000503:physiopathology*
D010455:Peptides