Isolation and characterisation of chicken L- and H-kininogens and their interaction with chicken cysteine proteinases and papain.
We have isolated L- and H-kininogens from chicken egg white and plasma using affinity chromatography on CM-papain Sepharose, Con-A Sepharose, gel filtration and ion exchange chromatography, in pure form. In chicken plasma mostly HK was present, whereas in chicken egg white both LK and HK were identified. The determined Mr of LK and HK were 69000 and about 96000, respectively. They occurred in multiple forms with pI 4.3-5.2. The inhibitory activity of chicken HK was tested against cysteine proteinases cathepsins B and L, isolated from chicken liver, and papain. The obtained Ki values demonstrated that chicken H-kininogen is a strong inhibitor of chicken cathepsin L and papain, but much weaker inhibitor of chicken cathepsin B.
Kos
J
J
Department of Biochemistry, J. Stefan Institute, Ljubljana, Slovenia.
Dolinar
M
M
Turk
V
V
D000595:Amino Acid Sequence; D000818:Animals; D002401:Cathepsin B / Q000037:antagonists & inhibitors; D056668:Cathepsin L; D002403:Cathepsins / Q000037:antagonists & inhibitors; D002642:Chick Embryo; D002645:Chickens / Q000378:metabolism*; D002845:Chromatography; D003546:Cysteine Endopeptidases; D015853:Cysteine Proteinase Inhibitors / Q000097:blood / Q000302:isolation & purification / Q000494:pharmacology; D004527:Egg Proteins / Q000302:isolation & purification; D010450:Endopeptidases; D007700:Kinetics; D007704:Kininogens / Q000097:blood / Q000302:isolation & purification* / Q000494:pharmacology; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010206:Papain / Q000037:antagonists & inhibitors
D015853:Cysteine Proteinase Inhibitors; D004527:Egg Proteins; D007704:Kininogens; D002403:Cathepsins; D010450:Endopeptidases; D003546:Cysteine Endopeptidases; D002401:Cathepsin B; D056668:Cathepsin L; D010206:Papain