Auxotyping of Neisseria gonorrhoeae as an additional epidemiological marker.
Auxotypes and penicillin sensitivity of 102 strains of N. gonorrhoeae were studied. Ten distinct auxotypes on the basis of growth requirement to seven amino acids were observed. The commonest pattern seen was zero auxotype (33.3%), followed by auxotype requiring proline (18.6%). MIC of 72 (71.5%) strains ranged from < 0.003 IU/ml to 0.062 IU/ml of benzyl penicillin. MIC of the other 30 (29.5%) strains was found to be > or = 0.125 IU/ml, indicating penicillin resistance. None of the strains were penicillinase producers. A case of reinfection was also detected on the basis of change in auxotype pattern.
Agarwal
S K
SK
Department of Microbiology, Lady Hardinge Medical College, Dr Ram Manohar Lohia Hospital, New Delhi.
Deb
M
M
Prakash
K
K
Sharma
A K
AK
D000596:Amino Acids / Q000378:metabolism; D001120:Arginine / Q000378:metabolism; D015373:Bacterial Typing Techniques; D004352:Drug Resistance, Microbial; D005260:Female; D006801:Humans; D008297:Male; D008826:Microbial Sensitivity Tests; D009344:Neisseria gonorrhoeae / Q000145:classification* / Q000378:metabolism; D011392:Proline / Q000378:metabolism
D000596:Amino Acids; D001120:Arginine; D011392:Proline