New plant binary vectors with selectable markers located proximal to the left T-DNA border.
Five new binary vectors have been constructed which have the following features: (1) different plant selectable markers including neomycin phosphotransferase (nptII), hygromycin phosphotransferase (hpt), dihydrofolate reductase (dhfr), phosphinothricin acetyl transferase (bar), and bleomycin resistance (ble); (2) selectable markers are located near the T-DNA left border and; (3) selectable marker and beta-glucuronidase (uidA) reporter genes are divergently organized for efficient expression, and can easily be removed or replaced as needed.
Becker
D
D
Max-Planck-Institut für Züchtungsforschung, Köln, Germany.
Kemper
E
E
Schell
J
J
Masterson
R
R
D016960:Agrobacterium tumefaciens / Q000235:genetics*; D017360:Arabidopsis / Q000235:genetics; D005819:Genetic Markers; D005822:Genetic Vectors; D012641:Selection, Genetic; D014170:Transformation, Genetic
D005819:Genetic Markers