Analyses of mitochondrial respiratory chain function and mitochondrial DNA deletion in human skeletal muscle: effect of ageing.
The analysis of human skeletal muscle mitochondria revealed a progressive decline in mitochondrial respiratory chain function with age. The activities affected to the greatest extent were those of complexes I and IV which were decreased by 59% and 47% respectively between the ages of 20-30 years and 60-90 years of age. Quantitation of the 5 kb 'common' deletion of mtDNA using PCR revealed a progressive accumulation with age, from approximately 1 in 100,000 at 21 years to 1 in 10,000 at 56 years and 1 in 5000 at 78 years of age. The low absolute levels of this mutation are unlikely to contribute significantly to the observed mitochondrial dysfunction.
Cooper
J M
JM
Department of Neuroscience, Royal Free Hospital School of Medicine, London, UK.
Mann
V M
VM
Schapira
A H
AH
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000378:metabolism / Q000502:physiology*; D001483:Base Sequence; D015139:Blotting, Southern; D004247:DNA / Q000235:genetics* / Q000378:metabolism; D004579:Electron Transport / Q000502:physiology; D017353:Gene Deletion; D006801:Humans; D008875:Middle Aged; D008931:Mitochondria, Muscle / Q000378:metabolism*; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes / Q000235:genetics; D010101:Oxygen Consumption; D011048:Polarography; D016133:Polymerase Chain Reaction
D015345:Oligonucleotide Probes; D004247:DNA