Anomalous phosphorylated neurofilament aggregations in central and peripheral axons of hens treated with tri-ortho-cresyl phosphate (TOCP).
Previous biochemical studies demonstrated a dramatic increase in phosphorylation of cytoskeletal proteins that occurs early in organophosphorus ester-induced delayed neurotoxicity (OPIDN). In this report we present immunohistochemical evidence that there is anomalous aggregation of phosphorylated neurofilaments within central and peripheral axons following organophosphate exposure. The morphology, location, and time of appearance of these aggregations are consistent with the hypothesis that the aberrant phosphorylation of cytoskeletal elements is an antecedent to the focal axonal swelling and degeneration characteristic of OPIDN.
Jensen
K F
KF
Neurotoxicology Division, U.S. Environmental Protection Agency, Research Triangle Park, NC 27709.
Lapadula
D M
DM
Anderson
J K
JK
Haykal-Coates
N
N
Abou-Donia
M B
MB
D000818:Animals; D000911:Antibodies, Monoclonal; D001369:Axons / Q000187:drug effects* / Q000378:metabolism; D002645:Chickens; D005260:Female; D007150:Immunohistochemistry; D007382:Intermediate Filaments / Q000187:drug effects* / Q000378:metabolism; D010525:Peripheral Nerves / Q000187:drug effects; D010766:Phosphorylation; D012584:Sciatic Nerve / Q000187:drug effects / Q000378:metabolism; D013116:Spinal Cord / Q000166:cytology / Q000187:drug effects; D014317:Tritolyl Phosphates / Q000633:toxicity*
D000911:Antibodies, Monoclonal; D014317:Tritolyl Phosphates; C025541:tri-o-cresyl phosphate