title: Elements in the first intron of the alpha 1(I) collagen gene interact with Sp1 to regulate gene expression.
abstract: Sequences within the first intron of the alpha 1(I) collagen gene act both positively and negatively to regulate expression of the gene. We have further characterized a 274 bp intronic sequence that contains an orientation-specific inhibitory activity and represents a constitutive DNase I-hypersensitive site in the gene. We show that this sequence contains two tandem, unique binding elements for the transcription factor Sp1. In addition, an Sp1-like site, capable of competing for protein binding to the intronic elements, resides in the distal promoter of the collagen gene. The results of experiments with site-directed mutations that abolish binding to the intronic elements indicate that these protein-DNA interactions have an inhibitory effect on the transcriptional efficiency of alpha 1(I) collagen-reporter gene constructs in transient transfection analysis. These data support our conclusion that the first intron plays a complex role, involving multiple protein-DNA binding interactions, in the regulation of expression of the alpha 1(I) collagen gene.
authors: Department of Biochemistry, University of Washington, Seattle 98195.
authors: D J
authors: DJ
authors: Liska
authors: V R
authors: VR
authors: Robinson
authors: P
authors: P
authors: Bornstein
mesh_terms: D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002460:Cell Line; D002645:Chickens; D003094:Collagen / Q000235:genetics*; D004247:DNA / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism; D005786:Gene Expression Regulation / Q000235:genetics*; D006801:Humans; D007438:Introns; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D012045:Regulatory Sequences, Nucleic Acid; D016415:Sequence Alignment; D016329:Sp1 Transcription Factor / Q000378:metabolism*
chemical_list: D016329:Sp1 Transcription Factor; D003094:Collagen; D004247:DNA