title: In vitro assessment of cytotoxicity and biotransformation of propranolol in Cunninghamella echinulata.
abstract: 1. Biotransformation studies with five concentrations of racemic propranolol were conducted using the filamentous fungus Cunninghamella echinulata ATCC 9244. 2. The rate of formation and subsequent disappearance of a new major metabolite, 8-hydroxypropranolol, was dose-dependent. Desisopropylpropranolol and 4-hydroxypropranolol were also formed. 4-Hydroxypropranolol was the major fungal metabolite in earlier studies. 3. Propranolol exerted a dose-dependent response on biotransformation, fungal growth, dextrose utilization, ammonia formation and incubation broth pH. Determination of dextrose utilization and incubation broth pH would provide reliable, cost-effective and convenient alternative methods for cytotoxicological evaluation.
authors: Bureau of Drug Research, Health Protection Branch, Ottawa, Ontario, Canada.
authors: B C
authors: BC
authors: Foster
authors: D L
authors: DL
authors: Litster
authors: D L
authors: DL
authors: Wilson
authors: E
authors: E
authors: Ormsby
authors: B A
authors: BA
authors: Dawson
mesh_terms: D000641:Ammonia / Q000378:metabolism; D001711:Biotransformation; D002852:Chromatography, Ion Exchange; D003603:Cytotoxins / Q000633:toxicity*; D008401:Gas Chromatography-Mass Spectrometry; D005947:Glucose / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D009090:Mucorales / Q000187:drug effects* / Q000378:metabolism; D011433:Propranolol / Q000493:pharmacokinetics / Q000633:toxicity*; D013237:Stereoisomerism
chemical_list: D003603:Cytotoxins; D000641:Ammonia; D011433:Propranolol; D005947:Glucose