Human chorionic beta-mannosidase: comparison with beta-mannosidase from human cultured fibroblasts.
The conditions for assay of beta-mannosidase activity in human chorionic villi (CV) were studied using the fluorogenic substrate 4-methylumbelliferyl-beta-D-mannopyranoside. A comparison of the biochemical properties of the CV beta-mannosidase with those of the enzyme from human cultured fibroblasts showed their similarity. Like the enzyme from skin fibroblasts, the CV beta-mannosidase had rather high activity. Both enzymes had virtually the same pH optimum (4.2-4.7) and Km value. The data presented suggest that chorion biopsy specimens can be used for prenatal determination of beta-mannosidase activity at an early stage of development.
Petushkova
N A
NA
Institute of Biological and Medical Chemistry, Russian Academy of Medical Sciences, Moscow.
Ivleva
T S
TS
Vozniy YaM
D002478:Cells, Cultured / Q000201:enzymology; D002824:Chorionic Villi / Q000201:enzymology*; D005260:Female; D005347:Fibroblasts / Q000201:enzymology; D006801:Humans; D008361:Mannosidases / Q000172:deficiency / Q000378:metabolism*; D011247:Pregnancy; D011296:Prenatal Diagnosis; D008363:alpha-Mannosidosis / Q000175:diagnosis*; D044902:beta-Mannosidase
D008361:Mannosidases; D044902:beta-Mannosidase