Reagent for introducing pyrene residues in oligonucleotides.
A novel pyrenyl-containing phosphoramidite reagent, N-[4-(1-pyrenyl)butyryl]-O1-(4,4'-dimethoxytrityl)-O2- [(diisopropylamino)(2-cyanoethoxy)phosphino]-3-amino-1 ,2-propanediol (5), has been synthesized from 4-(1-pyrenyl)butanoic acid in four steps with the 52% overall yield and used to incorporate pyrene residue(s) into oligonucleotides. Oligonucleotides 6 and 7, bearing one or two pyrenes at the 5'-terminus, have been prepared by means of that reagent, characterized with fluorescence spectra, and successfully used as primers in a polymerase chain reaction.
Korshun
V A
VA
M. M. Shemyakin Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow.
Pestov
N B
NB
Birikh
K R
KR
Berlin
Y A
YA
D001483:Base Sequence; D004591:Electrophoresis, Polyacrylamide Gel; D007202:Indicators and Reagents; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000737:chemistry*; D016133:Polymerase Chain Reaction; D011721:Pyrenes / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
D007202:Indicators and Reagents; D009841:Oligonucleotides; D011721:Pyrenes; C078687:N-(4-(1-pyrenyl)butyryl)-O(1)-(4,4'-dimethoxytrityl)-O(2)-((diisopropylamino)(2-cyanoethoxy)phosphino)-3-amino-1,2-propanediol