[Immunodiagnosis of Taenia saginata cysticercosis].
Many different serological tests have been investigated in order to replace the current "knife and eye" method at the slaughterhouse, which detects only a small fraction of the cattle infected with cysticercosis. Among the assays for the detection of antibodies against the cysticerci of Taenia saginata, the ELISA is certainly the most sensitive, although it is unable to detect lightly infected animals. Consequently it only allows a group diagnosis in naturally infected cattle. The use of purified antigens of T. saginata, T. crassiceps and T. hydatigena did not improve significantly the reliability of these antibody detection tests. Recently monoclonal antibodies have been developed against surface or excretory- and secretory antigens of T. saginata metacestodes, allowing the detection of circulating antigens in the serum of infected cattle. Furthermore these monoclonal antibodies can distinguish animals carrying living, infectious cysticerci from those harbouring only dead cysticerci. Research is currently going on in order to improve the sensitivity of this antigen detection test, so that it can replace the current unreliable meat inspection methods in the slaughterhouse.
Geerts
S
S
Prins Leopold Instituut voor Tropische Geneeskunde, Antwerpen.
Brandt
J
J
Kumar
V
V
De Deken
R
R
D000818:Animals; D000909:Antibodies, Helminth / Q000302:isolation & purification*; D000911:Antibodies, Monoclonal; D002417:Cattle / Q000469:parasitology*; D003551:Cysticercosis / Q000469:parasitology; D004797:Enzyme-Linked Immunosorbent Assay; D005513:Food Inspection / Q000379:methods; D008460:Meat / Q000469:parasitology*; D013621:Taenia / Q000276:immunology*
D000909:Antibodies, Helminth; D000911:Antibodies, Monoclonal
De immunodiagnose van Taenia saginata cysticerkose.