In situ DNA-protein binding: a novel method for detecting DNA-binding activity of transcription factor in brain.
A novel method, in situ DNA-protein binding (in situ DPB), was developed to detect the distribution and DNA-binding activity of AP-1 and Sp1 binding proteins in situ. The regional distribution of AP-1 binding protein in mouse brain was different from that of Sp1. Antibody against the DNA-binding domain of Jun protein markedly reduced the AP-1 but not the Sp1 binding activity. The binding activity of AP-1 probe increased markedly in the brain after administration of methamphetamine. These results suggest that the in situ DPB is convenient and sensitive for detecting the distribution and the DNA-binding activity of transcription factors in situ.
Wang
X B
XB
Department of Pharmacology, Osaka University Medical School, Japan.
Watanabe
Y
Y
Osugi
T
T
Ikemoto
M
M
Hirata
M
M
Miki
N
N
D000818:Animals; D001483:Base Sequence; D001923:Brain Chemistry / Q000502:physiology*; D004247:DNA / Q000378:metabolism*; D008694:Methamphetamine / Q000494:pharmacology; D051379:Mice; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D011485:Protein Binding; D016760:Proto-Oncogene Proteins c-fos / Q000096:biosynthesis; D016755:Proto-Oncogene Proteins c-jun / Q000378:metabolism; D016329:Sp1 Transcription Factor / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism*
D009419:Nerve Tissue Proteins; D016760:Proto-Oncogene Proteins c-fos; D016755:Proto-Oncogene Proteins c-jun; D016329:Sp1 Transcription Factor; D014157:Transcription Factors; D008694:Methamphetamine; D004247:DNA