title: Application of pyrolysis mass spectrometry to the investigation of outbreaks of food poisoning and non-gastrointestinal infection associated with Bacillus species and Clostridium perfringens.
abstract: Eighteen isolates of Bacillus species and 15 of Clostridium perfringens, all of which had been associated with outbreaks of either food poisoning or non-gastrointestinal infection (NGI), were examined for relatedness by pyrolysis mass spectrometry (PyMS). The PyMS-analysis correctly clustered all the groups of epidemiologically related isolates of both genera, and distinguished all the single, epidemiologically unrelated isolates of the same species. PyMS is a simple, rapid and inexpensive technique which can provide useful and accurate inter-strain comparisons within both the Bacillus and Clostridium genera in complete accord with conventional serological typing results.
authors: Regional Public Health Laboratory, General Hospital, Newcastle upon Tyne, England, UK.
authors: P R
authors: PR
authors: Sisson
authors: J M
authors: JM
authors: Kramer
authors: M M
authors: MM
authors: Brett
authors: R
authors: R
authors: Freeman
authors: R J
authors: RJ
authors: Gilbert
authors: N F
authors: NF
authors: Lightfoot
mesh_terms: D016863:Bacillaceae Infections / Q000382:microbiology*; D001407:Bacillus / Q000145:classification*; D003015:Clostridium Infections / Q000382:microbiology*; D003016:Clostridium perfringens / Q000145:classification*; D016000:Cluster Analysis; D004196:Disease Outbreaks; D005517:Foodborne Diseases / Q000382:microbiology*; D006801:Humans; D013058:Mass Spectrometry