High-performance liquid chromatographic method for the determination of tris(hydroxymethyl)aminomethane (tromethamine) in human plasma.
Determination of tris(hydroxymethyl)aminomethane (tromethamine) in human plasma involved derivatization of the amino and hydroxyl groups with a ultraviolet-absorbing chromophore followed by extraction into an organic phase. Reversed-phase high-performance liquid chromatography with gradient elution was used for the separation of the analyte from the internal standard (2,3-butanediol). The assay was linear in the range 1.0-1000.0 micrograms/ml of plasma and the coefficient of variation varied between 9.6 and 16.3% whereas the accuracy varied between 90 and 108%. The limit of detection for the assay was 0.282 micrograms/ml. Stability of tris(hydroxymethyl)aminomethane in human plasma frozen at -20 degrees C was studied over a period of three month and the data indicated no significant change.
Gumbhir
K
K
Pharmacokinetics Laboratory, School of Pharmacy, University of Missouri-Kansas City 64108.
Mason
W D
WD
D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007202:Indicators and Reagents; D012044:Regression Analysis; D013056:Spectrophotometry, Ultraviolet; D014325:Tromethamine / Q000032:analysis*
D007202:Indicators and Reagents; D014325:Tromethamine