Haemonchus contortus resistance to ivermectin and netobimin in Brazilian sheep.
Suffolk, Texel, Hampshire Down and Ile de France sheep from the municipalities of Porto Amazonas, Piraquara and Araucaria in the State of Paraná, and Bagé in the State of Rio Grande do Sul were brought to Sobral, State of Ceará, to be used in a cross-breeding project. On arrival they had clinical signs of nematode parasitosis, and one Suffolk female died. The animals were treated orally with ivermectin (0.2 mg kg-1) and fifteen days later with netobimin (20.0 mg kg-1). Neither drug reduced the egg counts (measured in eggs per gram, EPG) significantly, and this suggested that the nematodes in the sheep were resistant to the anthelmintics used. Haemonchus contortus was the species involved. The egg counts were reduced after oral treatment with trichlorfon (100.0 mg kg-1). Haemonchus contortus larvae obtained from these animals before trichlorfon treatment and passaged through two nematode-free sheep were used in a further experiment. Twenty 6- to 9-month-old nematode-free lambs were infected with the H. contortus larvae (10,000 per animal) and after the infection was confirmed, were randomly divided into four groups of five animals. Group I was orally treated with ivermectin at 0.2 mg kg-1, Group II with oral netobimin at 20.0 mg kg-1, Group III with oral trichlorfon at 100.0 mg kg-1 and Group IV was a non-treated control. Egg counts and faecal cultures were taken before dosing on the day of treatment and seven days later when all animals were necropsied and the nematodes were collected from the abomasa and counted.(ABSTRACT TRUNCATED AT 250 WORDS)
Vieira
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LS
Centro Nacional de Pesquisa de Caprinos (EMBRAPA-CNPC), Sobral, CE, Brazil.
Berne
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ME
Cavalcante
A C
AC
Costa
C A
CA
D000284:Administration, Oral; D000818:Animals; D001938:Brazil; D004351:Drug Resistance; D005243:Feces / Q000469:parasitology; D005260:Female; D006146:Guanidines / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D006188:Haemonchiasis / Q000188:drug therapy / Q000469:parasitology / Q000662:veterinary*; D006189:Haemonchus / Q000187:drug effects*; D007559:Ivermectin / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D010270:Parasite Egg Count; D011897:Random Allocation; D012756:Sheep; D012757:Sheep Diseases / Q000188:drug therapy* / Q000469:parasitology; D014236:Trichlorfon / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use
D006146:Guanidines; D007559:Ivermectin; D014236:Trichlorfon; C049626:netobimin