Oligonucleotide labeling methods. 3. Direct labeling of oligonucleotides employing a novel, non-nucleosidic, 2-aminobutyl-1,3-propanediol backbone.
Novel CE-phosphoramidite (7a-e) and CPG (8a, c, d, e) reagents have been prepared from a unique 2-aminobutyl-1,3-propanediol backbone. The reagents have been used to directly label oligonucleotides with fluorescein, acridine, and biotin via automated DNA synthesis. The versatile 2-aminobutyl-1,3-propanediol backbone allows for labeling at any position (5', internal, and 3') during solid phase oligonucleotide synthesis. Multiple labels can be achieved by repetitive coupling cycles. Furthermore, the 3-carbon atom internucleotide phosphate distance is retained when inserted internally. Using this method, individual oligonucleotides possessing two and three different reporter molecules have been prepared.
Nelson
P S
PS
Nucleic Acids Chemistry Division, Clontech Laboratories, Inc., Palo Alto, CA 94303.
Kent
M
M
Muthini
S
S
D001331:Automation; D001483:Base Sequence; D004247:DNA / Q000138:chemical synthesis; D007202:Indicators and Reagents; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009838:Oligodeoxyribonucleotides / Q000032:analysis* / Q000737:chemistry; D011409:Propylene Glycols; D013873:Thionucleotides / Q000737:chemistry
D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides; D011409:Propylene Glycols; D013873:Thionucleotides; D004247:DNA