title: Quantitation of the novel anticonvulsant remacemide in rat and dog plasma and urine: application of the plasma methodology to measure the plasma protein binding of remacemide.
abstract: Sensitive and selective methods have been developed for quantitation of the novel anticonvulsant remacemide in rat and dog plasma and urine. The methods employed liquid-liquid extraction (urine) or ion-exchange solid-phase extraction (plasma), with an internal standard, followed by high-performance liquid chromatography with ultraviolet detection. The detection limit for both methods was 10 ng/ml. Overall accuracy was 0.00% for plasma and -1.4% for urine with a precision of 6.04 and 3.87% for plasma and urine, respectively. The standard curves were linear for both plasma and urine over a wide concentration range (9.96-2490 ng/ml). The plasma method was also applied to measurement of in vitro plasma protein binding of remacemide in rat, dog and human plasma.
authors: Department of Preclinical Research Support, Fisons Pharmaceutical Division, Rochester, NY 14623.
authors: T C
authors: TC
authors: Wilson
authors: M S
authors: MS
authors: Eisman
authors: G E
authors: GE
authors: Machulskis
mesh_terms: D000081:Acetamides / Q000097:blood* / Q000652:urine*; D000818:Animals; D000927:Anticonvulsants / Q000097:blood* / Q000652:urine*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004285:Dogs; D006801:Humans; D011485:Protein Binding; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000081:Acetamides; D000927:Anticonvulsants; C067553:remacemide