title: Cellular repopulation of deep-frozen meniscal autografts: an experimental study in the dog.
abstract: This study evaluated the cellular repopulation of deep-frozen meniscal autografts. Medial menisci of adult dogs were excised, deep-frozen in liquid nitrogen (-196 degrees C) for 10 min, and orthotopically reimplanted into the joint. Deep-freezing was found to effectively kill all the cells within the meniscus as determined by the absence of Na(2)35SO4 incorporation. Following orthotopic replacement within the knee joint, menisci were repopulated with cells that seemed to originate from the adjacent synovium. These cells migrated over the surface of the meniscus and began to invade the deeper layers of the tissue. However, even after 6 months, the central core of the meniscus remained acellular. While the new cells appeared to modulate into cells that are similar in appearance to meniscal fibrochondrocytes, the exact phenotypic expression of these newly differentiated cells has yet to be determined. Histological alterations, as manifested by a loss of normal orientation of the collagen architecture of the superficial layers of the meniscus; was evident at 6 months and suggests that a remodeling phenomenon may be associated with the cellular repopulation. While biomaterial studies have not been carried out on these specimens, the morphologic alterations observed in the collagen orientation would suggest a possible alteration in the material properties of the repopulated meniscus. The clinical implication of this study is that the structural remodeling associated with the cellular repopulation of deep-frozen meniscal allografts may make the transplanted meniscus more susceptible to injury.
authors: Laboratory for Comparative Orthopaedic Research, Hospital for Special Surgery, New York, New York.
authors: S P
authors: SP
authors: Arnoczky
authors: E F
authors: EF
authors: DiCarlo
authors: S J
authors: SJ
authors: O'Brien
authors: R F
authors: RF
authors: Warren
mesh_terms: D000818:Animals; D001345:Autoradiography; D015925:Cryopreservation; D004195:Disease Models, Animal; D004285:Dogs; D008592:Menisci, Tibial / Q000166:cytology / Q000502:physiology* / Q000601:surgery / Q000637:transplantation*; D012038:Regeneration; D014182:Transplantation, Autologous