Identification of novel inducible nuclear factors that interact with the acute phase responsive promoter element of rabbit alpha 1-acid glycoprotein gene.
Alpha 1-acid glycoprotein (alpha 1-AGP) is a major acute phase protein that is highly inducible during hepatic acute-phase response. To identify the promoter element(s) required for increased gene transcription under acute condition a 529 bp 5'-flanking region of alpha 1-AGP gene is fused to the chloramphenicol acetyl transferase (CAT) reporter gene and introduced into BNL liver cells. This DNA fragment is capable of inducing synthesis of the reporter gene-product about ten-fold when transfected cells are exposed to conditioned medium from lipopolysaccharide-stimulated peripheral monocytes. Deletion analyses have shown that sequences located between -224 to -22 are capable of eliciting this inducible promoter function. Using electromobility shift assay we have identified two novel inducible nuclear factors from turpentine-induced acute liver that can interact with this regulatory promoter region. Our results indicate that binding of these two factors to the promoter region of alpha 1-AGP gene is highly specific in nature and the induction of this gene under acute condition may involve their participation.
Ray
B K
BK
Department of Veterinary Microbiology, College of Veterinary Medicine, University of Missouri, Columbia 65211.
Ray
A
A
D000818:Animals; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism*; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000378:metabolism*; D008099:Liver / Q000201:enzymology*; D009687:Nuclear Proteins / Q000096:biosynthesis / Q000378:metabolism*; D009961:Orosomucoid / Q000235:genetics*; D011401:Promoter Regions, Genetic; D011817:Rabbits; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D016385:TATA Box; D014158:Transcription, Genetic; D014162:Transfection
D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D009961:Orosomucoid; D011993:Recombinant Fusion Proteins; D015500:Chloramphenicol O-Acetyltransferase