[Cytotoxicity of ferric chloride used as an adjuvant of organic acid precipitation].
The objective of this study was the amelioration of the organic acid precipitation technique currently used as a secondary stage for viruses concentration using ferric chloride (FeCl3) as an adjuvant. To carry out this work successfully, we have studied the cytotoxicity effect of FeCl3 towards cell lines usually used for the investigation of enteric viruses. Two kinds of african green monkey kidney cells, MA104 and BGM, were used as a cellular model. The results showed that optimum concentration of FeCl3 for either BGM or MA104 cell survival and organic acid precipitation varies from 0.5 to 1 mM. The cytotoxicity test used in this work was simple, easy to realize and gives an appreciable information about the cytotoxicity dose of a given biological and chemical product.
Hassen
A
A
Institut National de Recherche Scientifique et Technique (INRST), Vandoeuvre, Nancy.
Hachicha
R
R
Jedidi
N
N
Agbalika
F
F
Harteman
P
P
D000818:Animals; D002460:Cell Line / Q000187:drug effects; D011232:Chemical Precipitation; D002712:Chlorides; D002522:Chlorocebus aethiops; D005069:Evaluation Studies as Topic; D005290:Ferric Compounds / Q000008:administration & dosage; D005428:Flocculation Tests / Q000379:methods / Q000592:standards*; D008253:Macaca mulatta; D014776:Virus Cultivation / Q000379:methods / Q000592:standards*
D002712:Chlorides; D005290:Ferric Compounds; C024555:ferric chloride
La cytotoxicité du chlorure ferrique utilisé comme adjuvant de la précipitation organique acide.