An in vitro evaluation of the effect of laser irradiation on the thrombogenicity of thrombus.
The effect of laser irradiation on the thrombogenicity of thrombus was evaluated by treating thrombi, formed in-vitro from canine blood, with two different doses of cw Nd:YAG laser energy at 1064 nm. The thrombi were then incubated with whole blood, and the plasma levels of fibrinogen and thrombin-antithrombin III-complexes were measured. A statistically significant decrease (p < 0.05) in the thrombogenicity was indicated by a reduction in both fibrinogen consumption and levels of thrombin-antithrombin III-complexes in the high dose group (600 joules, 100 degrees C peak temperature) in comparison to the low dose group (300 joules, 70 degrees C peak temperature) and the untreated thrombi. These findings suggest that laser irradiation of thrombus at an appropriate dose may substantially reduce its thrombogenicity and ability to modulate hemostasis.
Kundu
S K
SK
Division of Cardiology, Harper Hospital, Detroit, Michigan.
Ozawa
T
T
Patel
R H
RH
McMath
L P
LP
Mammen
E F
EF
Yellayi
S
S
Spears
J R
JR
D000818:Animals; D000990:Antithrombin III / Q000378:metabolism / Q000528:radiation effects*; D004285:Dogs; D005340:Fibrinogen / Q000378:metabolism / Q000528:radiation effects*; D066298:In Vitro Techniques; D007834:Lasers; D010447:Peptide Hydrolases / Q000378:metabolism / Q000528:radiation effects*; D013927:Thrombosis / Q000097:blood / Q000503:physiopathology*
C046193:antithrombin III-protease complex; D000990:Antithrombin III; D005340:Fibrinogen; D010447:Peptide Hydrolases