title: Localization of calcium in murine epidermis following disruption and repair of the permeability barrier.
abstract: Perturbation of the cutaneous permeability barrier results in rapid secretion of epidermal lamellar bodies, and synthesis and secretion of new lamellar bodies leading to barrier repair. Since external Ca2+ significantly impedes the repair response, we applied ion capture cytochemistry to localize Ca2+ in murine epidermis following barrier disruption. In controls, the numbers of Ca2+ precipitates in the basal layer were small, increasing suprabasally and reaching the highest density in the stratum granulosum. Barrier disruption with acetone produced an immediate, marked decrease in Ca2+ in the stratum granulosum, accompanied by secretion of lamellar bodies. Loss of this pattern of Ca2+ distribution was associated with the appearance of large Ca2+ aggregates within the intercellular spaces of the stratum corneum. The Ca(2+)-containing precipitates progressively reappeared in parallel with barrier recovery over 24 h. Disruption of the barrier with tape stripping also resulted in loss of Ca2+ from the nucleated layers of the epidermis, but small foci persisted where the stratum corneum was not removed; in these sites the Ca2+ distribution did not change and accelerated secretion of lamellar bodies was not observed. Following acetone-induced barrier disruption and immersion in isoosmolar sucrose, the epidermal Ca2+ gradient did not return, and both lamellar body secretion and barrier recovery occurred. However, with immersion in isoosmolar sucrose plus Ca2+, the epidermal Ca2+ reservoir was replenished, and both secretion of lamellar bodies and barrier recovery were impeded. These results demonstrate that barrier disruption results in loss of the epidermal Ca2+ reservoir, which may be the signal that initiates lamellar body secretion leading to barrier repair.
authors: Dermatology Service, Veterans Administration Medical Center, San Francisco, California 94121.
authors: G K
authors: GK
authors: Menon
authors: P M
authors: PM
authors: Elias
authors: S H
authors: SH
authors: Lee
authors: K R
authors: KR
authors: Feingold
mesh_terms: D000096:Acetone / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000032:analysis*; D004817:Epidermis / Q000378:metabolism* / Q000648:ultrastructure; D008297:Male; D051379:Mice; D008812:Mice, Hairless; D015388:Organelles / Q000378:metabolism; D010539:Permeability; D012996:Solutions; D013395:Sucrose; D014945:Wound Healing
chemical_list: D012996:Solutions; D000096:Acetone; D013395:Sucrose; D002118:Calcium