The L-isoaspartyl/D-aspartyl protein methyltransferase gene (PCMT1) maps to human chromosome 6q22.3-6q24 and the syntenic region of mouse chromosome 10.
We have mapped the genes for the human and mouse L-isoaspartyl/D-aspartyl protein carboxyl methyltransferase (EC 2.1.1.77) using cDNA probes. We determined that the human gene is present in chromosome 6 by Southern blot analysis of DNA from a panel of mouse-human somatic cell hybrids. In situ hybridization studies allowed us to confirm this identification and further localize the human gene (PCMT1) to the 6q22.3-6q24 region. By analyzing the presence of an EcoRI polymorphism in DNA from backcrosses of C57BL/6J and Mus spretus strains of mice, we localized the mouse gene (Pcmt-1) to chromosome 10, at a position 8.2 +/- 3.5 cM proximal to the Myb locus. This region of the mouse chromosome is homologous to the human 6q24 region.
MacLaren
D C
DC
Department of Chemistry and Biochemistry, University of California, Los Angeles 90024.
O'Connor
C M
CM
Xia
Y R
YR
Mehrabian
M
M
Klisak
I
I
Sparkes
R S
RS
Clarke
S
S
Lusis
A J
AJ
D000818:Animals; D002874:Chromosome Mapping; D002896:Chromosomes, Human, Pair 6; D004247:DNA; D008040:Genetic Linkage; D006801:Humans; D006822:Hybrid Cells; D017403:In Situ Hybridization; D051379:Mice; D008810:Mice, Inbred C57BL; D011110:Polymorphism, Genetic; D026601:Protein D-Aspartate-L-Isoaspartate Methyltransferase; D011496:Protein Methyltransferases / Q000235:genetics*
D004247:DNA; D011496:Protein Methyltransferases; D026601:Protein D-Aspartate-L-Isoaspartate Methyltransferase