Chromosomal localization of the ovine beta-casein gene by non-isotopic in situ hybridization and R-banding.
The ovine beta-casein gene (CNS2) has been mapped to a specific chromosome band using nonradioactive in situ hybridization and simultaneous fluorescent R-banding. The probe pTZ-E4 was a fragment of the ovine beta-casein gene inserted in the plasmid pTZ18R and labeled with biotin-11-dUTP. It hybridized to band q32 of ovine chromosome 4. The discrepancy between this result and the previous localization of this gene on cattle chromosome 6 may be explained by the very great similarity of the banding patterns of ovine and bovine chromosomes 4 and 6.
Hayes
H
H
Laboratoire de Cytogénétique, INRA-CRJ, Jouy-en-Josas, France.
Petit
E
E
Lemieux
N
N
Dutrillaux
B
B
D000818:Animals; D002364:Caseins / Q000235:genetics*; D002478:Cells, Cultured; D002871:Chromosome Banding; D002874:Chromosome Mapping; D017403:In Situ Hybridization; D008677:Metaphase; D012756:Sheep
D002364:Caseins