Subcellular localization of acid carboxypeptidase in rat liver and human fibroblasts.
The subcellular distribution of acid carboxypeptidase was investigated in rat liver, normal human skin (CRL 1501) and lung (WI-38) fibroblasts, galactosialidosis skin fibroblasts (GM 00806) and transformed lung fibroblasts (WI-38 VA 13). Results of differential and isopycnic centrifugations and osmotic activation experiments clearly indicate that the enzyme is located in lysosomes, in agreement with observations suggesting that carboxypeptidase is the protective protein of the 'Galjaard complex' which is defective in galactosialidosis.
Gérard
N
N
Thirion
J
J
Wattiaux-De Coninck
S
S
Wattiaux
R
R
D000818:Animals; D002268:Carboxypeptidases / Q000032:analysis*; D020789:Cathepsin C; D002460:Cell Line; D002461:Cell Line, Transformed; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases / Q000032:analysis; D005347:Fibroblasts / Q000201:enzymology* / Q000648:ultrastructure; D006801:Humans; D008099:Liver / Q000201:enzymology* / Q000648:ultrastructure; D008168:Lung / Q000166:cytology; D016464:Lysosomal Storage Diseases / Q000201:enzymology / Q000473:pathology; D051381:Rats; D012867:Skin / Q000166:cytology; D013347:Subcellular Fractions / Q000201:enzymology*
D002268:Carboxypeptidases; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases; D020789:Cathepsin C