Structural analysis of the N-linked oligosaccharides from human urinary trypsin inhibitor.
The N-linked oligosaccharides from human urinary trypsin inhibitor were purified and their structures were investigated by compositional analysis, the two-dimensional sugar map method and 500 MHz 1H-NMR. The results revealed that they were composed of disialosyl, monosialosyl and asialosyl oligosaccharides, which have the common biantennary core structure; Gal1-4GlcNAc1-2Man1-3(Gal1-4GlcNAc1-2Man1-6)M an1-4GlcNAc1-4GlcNAc.
Toyoda
H
H
Faculty of Pharmaceutical Sciences, Chiba University, Japan.
Ikei
T
T
Demachi
Y
Y
Toida
T
T
Imanari
T
T
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D006801:Humans; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry*; D014361:Trypsin Inhibitors / Q000737:chemistry* / Q000652:urine*
D009844:Oligosaccharides; D014361:Trypsin Inhibitors