The effect of active vitamin D3 analogs and dexamethasone on the expression of osteocalcin gene in rat tibiae in vivo.
We tested the effects of 1 alpha,25-dihydroxyvitamin D3 (1,25-(OH)2D3), 2 beta-(3-hydroxypropoxy)-1 alpha,25-dihydroxyvitamin D3 (ED-71) and dexamethasone on osteocalcin mRNA levels in rat tibiae in vivo. Northern blot analysis showed that both 1,25-(OH)2D3 and ED-71 caused an increase in osteocalcin mRNA levels in bone: 1,25-(OH)2D3 induced a transient increase in the mRNA levels followed by a decrease in the control level by 12 h post administration. In contrast, ED-71 caused a persistent increase in osteocalcin mRNA level for seven days post administration. Serum osteocalcin levels paralleled the osteocalcin mRNA level in bone in both groups. Dexamethasone caused a marked reduction in both osteocalcin mRNA and serum osteocalcin levels. Suppressive effect of dexamethasone on osteocalcin expression was persistent for seven days at higher dose. Our results represent the first demonstration of the effect of active vitamin D and corticosteroid on the expression of osteocalcin mRNA in bone in vivo.
Ikeda
T
T
Department of Orthopaedic Surgery, Kyoto University Faculty of Medicine, Japan.
Kohno
H
H
Yamamuro
T
T
Kasai
R
R
Ohta
S
S
Okumura
H
H
Konishi
J
J
Kikuchi
H
H
Shigeno
C
C
D000591:Amino Acid Isomerases / Q000235:genetics; D000704:Analysis of Variance; D000818:Animals; D015152:Blotting, Northern; D001842:Bone and Bones / Q000187:drug effects / Q000502:physiology*; D002117:Calcitriol / Q000031:analogs & derivatives* / Q000494:pharmacology*; D002352:Carrier Proteins / Q000235:genetics; D003524:Cyclosporins / Q000378:metabolism; D003907:Dexamethasone / Q000494:pharmacology*; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D007700:Kinetics; D015675:Osteocalcin / Q000096:biosynthesis / Q000097:blood / Q000235:genetics*; D019696:Peptidylprolyl Isomerase; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D013977:Tibia; D013997:Time Factors; D014807:Vitamin D / Q000031:analogs & derivatives
D002352:Carrier Proteins; D003524:Cyclosporins; D012333:RNA, Messenger; D015675:Osteocalcin; D014807:Vitamin D; D003907:Dexamethasone; D000591:Amino Acid Isomerases; D019696:Peptidylprolyl Isomerase; D002117:Calcitriol; C547512:eldecalcitol