title: Developmental stage- and tissue-specific expression of shibire, a Drosophila gene involved in endocytosis.
abstract: Dynamin, a microtubule-activated GTPase, has recently been identified as the product of the shibire gene in Drosophila. shi(ts) mutants are defective in synaptic vesicle recycling, which leads to rapid and reversible temperature-sensitive paralysis. In the present study, results from RNase protection assays and analysis of cDNA clones define a complex pattern of developmental- and tissue-specific alternative splicing at two sites within the coding region. This analysis is also supported by western blot analysis with two polyclonal antibodies. In situ hybridization data revealed a high concentration of shi transcripts in the central and peripheral nervous system throughout neuronal development. Other than the nervous system, shi transcripts are also expressed at a high level in early embryos, larval imaginal discs, and male and female gonads. These data provide a basis for interpreting the wide range of phenotypic effects of shi mutations in terms of the putative membrane-sorting properties of dynamin and for further functional study of different dynamin isoforms.
authors: Cell Biology Group, Worcester Foundation for Experimental Biology, Shrewsbury, MA 01545.
authors: M S
authors: MS
authors: Chen
authors: C C
authors: CC
authors: Burgess
authors: R B
authors: RB
authors: Vallee
authors: S C
authors: SC
authors: Wadsworth
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D017301:Ca(2+) Mg(2+)-ATPase / Q000378:metabolism; D004330:Drosophila / Q000196:embryology / Q000235:genetics*; D029721:Drosophila Proteins; D034281:Dynamins; D004705:Endocytosis / Q000235:genetics*; D005260:Female; D019204:GTP-Binding Proteins / Q000378:metabolism; D005786:Gene Expression Regulation; D006066:Gonads / Q000196:embryology / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D009420:Nervous System / Q000196:embryology / Q000378:metabolism; D012326:RNA Splicing
chemical_list: D029721:Drosophila Proteins; D017301:Ca(2+) Mg(2+)-ATPase; D019204:GTP-Binding Proteins; D034281:Dynamins; C439628:shi protein, Drosophila