Glutathione S-transferase-mediated metabolism of glyceryl trinitrate in subcellular fractions of bovine coronary arteries.
The possible role of glutathione S-transferases (GTSs) in vascular glyceryl trinitrate (GTN) metabolism was investigated. GTN degradation to form its dinitrate metabolites (GDNs) in the 9000g (9k) supernatant fraction of bovine coronary arteries (BCA) was examined. BCAs were homogenized with a 3x volume of phosphate buffer, and the 9k fraction was obtained by centrifugation. GTN (40 ng/ml; 1.76 x 10(-7) M) was incubated for 2 hr in the 9k fraction of BCA in the presence of reduced glutathione (2 x 10(-3) M). Samples were taken at 10, 20, 40, 60, and 120 min. GTN was observed to degrade readily, exhibiting a half-life of 26 min in the incubate. While both 1,2- and 1,3-GDNs were generated from GTN, formation of 1,3-GDN was predominant (GDN ratio, as 1,2/1,3-GDN, = 0.7-0.8). Coincubation with 2 x 10(-5) M concentrations of two GST inhibitors, sulfobromophthalein (SBP) and ethacrynic acid (ECA), decreased the rate of GTN loss. The GTN half-lives in SBP- and ECA-treated incubations were 66 and 84 min, respectively. In addition, the pattern of GDN formation was also altered. The resultant GDN ratios exceeded unity in the presence of these inhibitors, indicating that 1,3-GDN formation was attenuated to a greater extent than that of 1,2-GDN. These data suggest that vascular GTN metabolism in BCA is carried out by cytosolic GST isozymes which possess a preference for C-2 denitration of GTN.
Lau
D T
DT
Department of Pharmacy, School of Pharmacy, University of California, San Francisco 94143.
Chan
E K
EK
Benet
L Z
LZ
D000818:Animals; D002417:Cattle; D003331:Coronary Vessels / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism; D004976:Ethacrynic Acid / Q000494:pharmacology; D005982:Glutathione Transferase / Q000378:metabolism*; D066298:In Vitro Techniques; D007527:Isoenzymes / Q000378:metabolism; D008861:Microsomes / Q000378:metabolism; D005996:Nitroglycerin / Q000378:metabolism*; D012710:Serum Albumin, Bovine / Q000378:metabolism; D013448:Sulfobromophthalein / Q000494:pharmacology
D007527:Isoenzymes; D013448:Sulfobromophthalein; D012710:Serum Albumin, Bovine; D005982:Glutathione Transferase; D005996:Nitroglycerin; D004976:Ethacrynic Acid