title: Molecular analysis of immunoglobulin heavy chain genes coding for idiotypic and anti-idiotypic antibodies involved in B-B cellular interaction.
abstract: We recently reported that a unique B cell clone (B19-1d), specific for a cross-reactive idiotype (CRI) on MOPC104E myeloma protein (M104E), enhances Igh-restricted CRI+ antibody production. In this paper, we report the nucleotide sequences of immunoglobulin heavy chain variable regions (VH) of both M104E and B19-1d-derived hybridoma (HB19) antibodies. The sequence data revealed that both belong to the J558 germ line VH gene subfamily. Strikingly, not only the VH region, but also the leader sequences of M104E and HB19 are very similar to each other at 88% (VH) and 91% (leader) homology, but they use different D and J segments. The VH region sequence similarity is highest among the germ line VH gene sequences of the BALB/c J558 subfamily so far screened. Southern hybridization data, using 5'-noncoding regions of either M104E or HB19 genomic VH gene clones as probes, revealed that both VH genes are conserved in the M104E CRI producer strains of mice. Moreover, these probes show the restriction length polymorphism pattern of mouse VH genes in various strains. That the HB19 VH gene locates to the 5' upper arm of the M104E VH gene on the chromosome was suggested by Southern blot hybridization. Immunoglobulin VH gene restriction of idiotypic and antiidiotypic B-B cellular interaction is discussed from a molecular point of view.
authors: Department of Immunology, Kochi Medical School, Japan.
authors: S
authors: S
authors: Takahashi
authors: Y
authors: Y
authors: Matsuura
authors: T
authors: T
authors: Taniguchi
authors: H
authors: H
authors: Tamura
authors: S
authors: S
authors: Bitoh
authors: S
authors: S
authors: Onishi
authors: Y
authors: Y
authors: Yamamoto
authors: H
authors: H
authors: Yamamoto
authors: S
authors: S
authors: Fujimoto
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000235:genetics; D001402:B-Lymphocytes / Q000276:immunology*; D001483:Base Sequence; D002450:Cell Communication / Q000276:immunology*; D003001:Cloning, Molecular; D005803:Genes, Immunoglobulin / Q000235:genetics*; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D007130:Immunoglobulin Idiotypes / Q000235:genetics*; D051379:Mice; D008815:Mice, Inbred Strains; D008969:Molecular Sequence Data; D011994:Recombinant Proteins; D015183:Restriction Mapping; D016415:Sequence Alignment
chemical_list: D000888:Antibodies, Anti-Idiotypic; D007143:Immunoglobulin Heavy Chains; D007130:Immunoglobulin Idiotypes; D011994:Recombinant Proteins