The sequence within two primers influences the optimum concentration of dimethyl sulfoxide in the PCR.
Masoud
S A
SA
Department of Plant Pathology, Kansas State University, Manhattan 66506-5502.
Johnson
L B
LB
White
F F
FF
D001483:Base Sequence; D004274:DNA, Recombinant; D004121:Dimethyl Sulfoxide; D005813:Genes, Synthetic; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D030821:Plants, Genetically Modified; D010947:Plants, Toxic; D016133:Polymerase Chain Reaction / Q000379:methods*; D014026:Nicotiana / Q000235:genetics
D004274:DNA, Recombinant; D009838:Oligodeoxyribonucleotides; D004121:Dimethyl Sulfoxide