title: Gas chromatographic determination of 2- and 3-dechloroethylifosfamide in plasma and urine.
abstract: The metabolic oxidation of one of the chloroethyl groups of the antitumour drug ifosfamide leads to the formation of the inactive metabolites 2- and 3-dechloroethylifosfamide together with the neurotoxic metabolite chloroacetaldehyde. A very sensitive capillary gas chromatographic method, requiring only 50 microliters of plasma or urine, has been developed to measure the amounts of the drug and the two inactive metabolites in a single run. Calibration curves were linear (r > 0.999) in the concentration ranges from 50 ng/ml to 100 micrograms/ml in plasma and from 100 ng/ml to 1 mg/ml in urine.
authors: Department of Pharmaceutical Analysis, Faculty of Pharmacy, Utrecht University, Netherlands.
authors: G P
authors: GP
authors: Kaijser
authors: J H
authors: JH
authors: Beijnen
authors: A
authors: A
authors: Bult
authors: G
authors: G
authors: Wiese
authors: J
authors: J
authors: de Kraker
authors: H J
authors: HJ
authors: Keizer
authors: W J
authors: WJ
authors: Underberg
mesh_terms: D002849:Chromatography, Gas / Q000295:instrumentation / Q000379:methods*; D003110:Colonic Neoplasms / Q000097:blood / Q000188:drug therapy / Q000652:urine; D003520:Cyclophosphamide / Q000031:analogs & derivatives* / Q000032:analysis / Q000097:blood / Q000652:urine; D006801:Humans; D007069:Ifosfamide / Q000031:analogs & derivatives* / Q000032:analysis / Q000097:blood / Q000378:metabolism / Q000627:therapeutic use / Q000652:urine; D008297:Male; D008875:Middle Aged; D015203:Reproducibility of Results
chemical_list: C064445:dechloroethylcyclophosphamide; D003520:Cyclophosphamide; C038007:dechloroethylifosfamide; D007069:Ifosfamide