[Preparation and analysis of mutants of rhizospheric pseudomonads, resistant to toxic analogs of tryptophan and phenylalanine].
The absence of plasmids in strains of fluorescent pseudomonads characterized by high level of synthesis of phytohormone indole-3-acetic acid (IAA) as well as invariability of this feature in plasmid and non-plasmid variants of strain BSP8 suggests chromosomal control of IAA synthesis by the rhizosphere bacteria tested. Using toxic analogues of aromatic amino acids -5-fluorine-tryptophan and 5-methyl-tryptophan variants were obtained which synthesized and secreted only anthranilic acid. Mutants with resistance to p-fluorine-phenylalanine and capable of secreting tryptophan and/or phenylalanine were found. Testing of the secreting variants failed to reveal any differences between the levels of IAA biosynthesis in comparison with the wild-type strains.
Mordukhova
E A
EA
Dubeĭkovskiĭ
A N
AN
Kochetkov
V V
VV
Boronin
A M
AM
D002874:Chromosome Mapping; D004352:Drug Resistance, Microbial / Q000235:genetics; D007210:Indoleacetic Acids / Q000378:metabolism*; D009154:Mutation; D010957:Plasmids; D011549:Pseudomonas / Q000235:genetics*; D014364:Tryptophan / Q000031:analogs & derivatives* / Q000494:pharmacology; D010135:p-Fluorophenylalanine / Q000494:pharmacology*
D007210:Indoleacetic Acids; C004182:5-methyltryptophan; C026927:5-fluorotryptophan; D010135:p-Fluorophenylalanine; C030737:indoleacetic acid; D014364:Tryptophan
Poluchenie i analiz mutantov rizosfernykh psevdomonad, ustoĭchivykh k toksichnym analogam triptofana i fenilalanina.