Monoclonal antibodies against Nereis virens brain homogenates as probes for isolation and in situ detection of new neurosecretions.
The brain of Nereis contains 26 ganglionic nuclei which produce numerous neurosecretions. Only a few of them have been characterized. The production of monoclonal antibodies was adopted as an approach to discover unknown neurosecretions. Monoclonal antibodies produced against Nereis virens brain homogenates were selected using stepwise ELISA tests first with brain homogenates, then with brain neurosecretions. Eight antibodies specific for Nereis neurosecretions were selected. The results are illustrated with one of these monoclonal antibodies which was directed against a major peak after HPLC purification of brain neurosecretions. This antibody was subsequently used for the in situ detection of recognized epitope(s) in the brain and ventral nerve cord cells.
Delaire-Hesdin
A
A
Unité associée au C.N.R.S. 148, Endocrinologie Comparée des Invertébrés, Université de Lille Flandres-Artois, Villeneuve d'Ascq, France.
Bulet
P
P
Boilly-Marer
Y
Y
Porchet-Hennere
E
E
Masson
M
M
D000818:Animals; D000911:Antibodies, Monoclonal; D001923:Brain Chemistry; D004797:Enzyme-Linked Immunosorbent Assay; D005724:Ganglia / Q000737:chemistry; D009479:Neuropeptides / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification*; D009490:Neurosecretory Systems / Q000737:chemistry*; D011077:Polychaeta / Q000737:chemistry*
D000911:Antibodies, Monoclonal; D009479:Neuropeptides