Effect of amphotericin B on dopachrome tautomerase activity and other melanogenic parameters in cultured B16/F10 melanoma cells.
The antifungal reagent Fungizone (amphotericin B and deoxycholate) caused an activation in dopachrome tautomerase and dopa oxidase activities of B16/F10 melanoma cells at the routine concentration (2.5 micrograms/ml) used for preventing molds and yeast growth in cultures of animal cells. However, higher amphotericin B concentrations caused a significant cell death and the inhibition of enzymatic activities. At the optimal concentration of Fungizone, the enzymatic activities and melanin content were augmented as incubation time increased. The detergent sodium deoxycholate alone exerted no effect on these melanogenic parameters, eliminating the possibility that this detergent was partially responsible for melanogenic modifications produced by Fungizone. After withdrawal of Fungizone from the reaction medium, the recovery of melanogenic parameters to normal values was slower for DCT than for tyrosinase. The behavior of dopa oxidase was very similar to that reported by Johnson and Bagnara (Pigment Cell Res. 3, 173-175) for tyrosine hydroxylase.
Peinado
P
P
Departamento de Bioquímica y Biología Molecular, Facultad de Medicina, Universidad de Murcia, Spain.
Martínez-Liarte
J H
JH
Solano
F
F
Lozano
J A
JA
D000666:Amphotericin B / Q000008:administration & dosage / Q000494:pharmacology*; D016923:Cell Death / Q000187:drug effects; D003840:Deoxycholic Acid / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation / Q000187:drug effects; D019746:Intramolecular Oxidoreductases; D007535:Isomerases / Q000378:metabolism*; D007700:Kinetics; D008543:Melanins / Q000378:metabolism; D008546:Melanoma, Experimental / Q000201:enzymology*; D014442:Monophenol Monooxygenase / Q000378:metabolism; D014407:Tumor Cells, Cultured
D008543:Melanins; D003840:Deoxycholic Acid; D000666:Amphotericin B; D014442:Monophenol Monooxygenase; D007535:Isomerases; D019746:Intramolecular Oxidoreductases; C042501:dopachrome isomerase