On the potential of simple repetitive DNA for fingerprinting in clinical, forensic, and evolutionary dynamic studies.
The purpose of this review is to discuss critically the practical meaning of a specific genome component, simple repetitive desoxyribonucleic acid (DNA) sequences as clinical and forensic and diagnostic and research tools. Previously, multilocus DNA fingerprinting was the major technology employed to visualize such simple repeat sequences. This technique enables many polymorphic loci to be simultaneously detected thus yielding vast amounts of information. With the advent of enzymatic DNA amplification via the polymerase chain reaction (PCR), individual simple repeat loci can be demonstrated, theoretically even from single DNA molecules and so a wealth of additional approaches have also become feasible. In general investigating, small, known, single copy parts of genomes have not posed truly insurmountable problems if enough material was available. There have even been a few (anecdotal) reports on the amplification of simple repeats from ancient DNA (see, e.g. [30]. Here we would like to after a solid basis for an earnest discussion of the applications of these simple repetitive sequences using various methodological approaches relevant for clinical diagnosis, setting aside the obvious unsolved mysteries of their biology.
Epplen
J T
JT
Molekulare Humangenetik, Ruhr-Universität, Bochum.
Melmer
G
G
Schmidt
P
P
Roewer
L
L
Hundrieser
J
J
Epplen
C
C
Buitkamp
J
J
D000818:Animals; D016477:Artifacts; D005075:Biological Evolution; D016172:DNA Fingerprinting; D015342:DNA Probes; D004276:DNA, Satellite / Q000032:analysis*; D005544:Forecasting; D005554:Forensic Medicine / Q000379:methods*; D006801:Humans; D017403:In Situ Hybridization; D010334:Paternity; D016133:Polymerase Chain Reaction; D012091:Repetitive Sequences, Nucleic Acid; D012680:Sensitivity and Specificity
D015342:DNA Probes; D004276:DNA, Satellite