title: [Rare codons and gene expression in Escherichia coli].
abstract: Influence of rare codons upon gene expression in E. coli was investigated. The chimeric gene was created combining CAT gene and a fragment of the gene, encoding for alpha-domain of beta-galactosidase. The synthetic oligonucleotides were inserted in different parts of the chimeric gene. The constructed synthetic oligonucleotides encoded the same amino acid sequences and contained arginine codons AGG, AGA and CGT in various combinations. It was shown that the presence of rare arginine codons AGG and AGA in the template and their mutual arrangement significantly influence the level of gene expression. At the same time the presence of leucine, isoleucine, glycine and proline rare codons does not cause such an effect. Translation of AGGAGG and AGAAGA sequences was found to lead to the formation of a considerable amount of polypeptides of incomplete length. It was shown that the presence of such a cluster of rare codons effects on the length of specific mRNA.
authors: Ia G
authors: IaG
authors: Gurskiĭ
authors: N Iu
authors: NIu
authors: Marimont
authors: A Ia
authors: AIa
authors: Shevelev
authors: A A
authors: AA
authors: Iuzhakov
authors: R Sh
authors: RSh
authors: Bibilashvili
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002678:Chimera; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D003062:Codon; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics*; D015870:Gene Expression; D008969:Molecular Sequence Data; D010957:Plasmids; D001616:beta-Galactosidase / Q000235:genetics
chemical_list: D003062:Codon; D015500:Chloramphenicol O-Acetyltransferase; D001616:beta-Galactosidase
vernacular_title: Redkie kodony i ékspressiia genov v Escherichia coli.