Hormonal control of in vitro vitellogenin synthesis in Rana esculenta liver: effects of mammalian and amphibian growth hormone.
Estradiol 17-beta is known to induce hepatic synthesis and secretion of vitellogenin in all species studied and in Rana esculenta, previous experiments demonstrated the involvement of pituitary in these processes; indeed, in addition to estradiol 17-beta, homologous pituitary homogenate directly stimulated male and female liver to produce vitellogenin in tissue cultures. Therefore, the effect of ovine growth hormone (o-GH) and Rana catesbeiana growth hormone (f-GH) on hepatic vitellogenin synthesis was investigated. In the present in vitro experiments, both o-GH and f-GH positively stimulated vitellogenin synthesis, in female and male liver, in a dose-related fashion. No significant differences were found in VTG levels induced by o-GH and f-GH. The GH stimulatory effects, found during the different phases of the reproductive cycle, displayed different trends related to season and sex.
Carnevali
O
O
Department of Biology (MCA) University of Camerino, Italy.
Mosconi
G
G
Yamamoto
K
K
Kobayashi
T
T
Kikuyama
S
S
Polzonetti-Magni
A M
AM
D000818:Animals; D003470:Culture Media; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006728:Hormones / Q000502:physiology*; D008099:Liver / Q000378:metabolism* / Q000502:physiology; D008297:Male; D009924:Organ Culture Techniques; D011892:Rana catesbeiana; D011893:Rana esculenta; D012727:Sex Characteristics; D014819:Vitellogenins / Q000096:biosynthesis*
D003470:Culture Media; D006728:Hormones; D014819:Vitellogenins