Sequence analysis of cucumber cotyledon ribulosebisphosphate carboxylase/oxygenase activase cDNA.
The cDNA encoding ribulosebisphosphate carboxylase/oxygenase activase from cotyledons of dark-grown cucumber seedlings has been isolated and sequenced. The 2.0 kilobase pair cDNA encodes a polypeptide with 413 amino acid residues which shows 70% identity with the rubisco activase from green leaves of spinach. The full-length cDNA was used to prepare rubisco activase by in vitro transcription. Subsequent in vitro translation led to the formation of a 46 kDa protein which is the putative precursor of a 42 kDa plastid enzyme.
Preisig-Müller
R
R
Biochemie, FB Chemie, Philipps-Universität, Marburg, Germany.
Kindl
H
H
D000595:Amino Acid Sequence; D001483:Base Sequence; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010940:Plant Proteins; D010944:Plants / Q000201:enzymology* / Q000235:genetics*; D014176:Protein Biosynthesis; D012273:Ribulose-Bisphosphate Carboxylase / Q000235:genetics*; D014158:Transcription, Genetic
D010940:Plant Proteins; C054765:rca protein, plant; D004247:DNA; D012273:Ribulose-Bisphosphate Carboxylase