Guanosine enhances NGF-stimulated neurite outgrowth in PC12 cells.
Guanosine at 30 and 300 microM elicited the de novo extension of neurites from PC12 cells. With saturating concentrations of NGF, guanosine acted in a synergistic manner to enhance neuritogenesis. Adenosine alone also stimulated neurite outgrowth, but did not enhance NGF-induced neuritogenesis. 5'-N-ethylcarboxamidoadenosine (NECA), an adenosine analog and A1/A2 receptor agonist, also alone had neuritogenic effects. It enhanced NGF-induced neuritic outgrowth but not to the same extent as guanosine. However, when NECA was added together with guanosine in the presence of NGF, these compounds elicited a greatly enhanced neuritogenic response. This suggested that the mechanisms through which NECA modulates the neuritogenic effects may be different from those of guanosine and NGF.
Gysbers
J W
JW
Department of Biomedical Sciences, McMaster University Health Science Centre, Hamilton, Ontario, Canada.
Rathbone
M P
MP
D000241:Adenosine / Q000031:analogs & derivatives / Q000494:pharmacology; D019830:Adenosine-5'-(N-ethylcarboxamide); D000818:Animals; D004357:Drug Synergism; D006151:Guanosine / Q000494:pharmacology*; D009414:Nerve Growth Factors / Q000494:pharmacology*; D016501:Neurites / Q000187:drug effects*; D016716:PC12 Cells; D011983:Receptors, Purinergic / Q000187:drug effects; D014665:Vasodilator Agents / Q000494:pharmacology
D009414:Nerve Growth Factors; D011983:Receptors, Purinergic; D014665:Vasodilator Agents; D006151:Guanosine; D019830:Adenosine-5'-(N-ethylcarboxamide); D000241:Adenosine