Hepatocyte growth factor is linked by O-glycosylated oligosaccharide on the alpha chain.
The glycosylation site and the structure of O-glycosylated oligosaccharide of recombinant human HGF were investigated. N-acetylgalactosamine (GalNAc) in the alpha chain suggested the presence of O-glycosylated oligosaccharide. Sugar analysis and amino acid sequence analysis of peptide fragments produced by limited degradation revealed that O-glycosylated oligosaccharide linked to Thr445 of the alpha chain. The molecular weight of the oligosaccharide was determined with ion spray mass spectrometry. From these studies, the structure of the O-glycosylated oligosaccharide on the alpha chain of HGF was concluded as [formula: see text].
Shimizu
N
N
Pharmaceuticals Research Center, Toyobo Co. Ltd., Otsu, Japan.
Hara
H
H
Sogabe
T
T
Sakai
H
H
Ihara
I
I
Inoue
H
H
Nakamura
T
T
Shimizu
S
S
D000595:Amino Acid Sequence; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D003545:Cysteine; D006031:Glycosylation; D017228:Hepatocyte Growth Factor / Q000737:chemistry*; D006801:Humans; D008239:Lysine; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D009005:Monosaccharides / Q000032:analysis; D009844:Oligosaccharides / Q000737:chemistry*; D010446:Peptide Fragments / Q000737:chemistry / Q000302:isolation & purification; D011994:Recombinant Proteins / Q000737:chemistry
D046911:Macromolecular Substances; D009005:Monosaccharides; D009844:Oligosaccharides; D010446:Peptide Fragments; D011994:Recombinant Proteins; D017228:Hepatocyte Growth Factor; D008239:Lysine; D003545:Cysteine