Production of a monoclonal antibody that recognizes the lipopolysaccharide of a Campylobacter-like organism.
A monoclonal antibody was produced to a Campylobacter-like organism (RMIT 32A) which was isolated from the terminal ileum of a pig with proliferative enteritis. Isotyping of the antibody revealed that it was an IgG2a with kappa light chains. Immunoblots using the antibody against proteinase-K-treated whole cell lysates of RMIT 32A, a selection of Campylobacter species and other enteric bacteria showed that the antibody was specific for RMIT 32A and was directed against the lipopolysaccharide. This antibody can be used for the specific detection of RMIT 32A.
Kuan
S K
SK
Department of Applied Biology and Biotechnology, Royal Melbourne Institute of Technology, Australia.
Coloe
P J
PJ
Alderton
M R
MR
D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D015153:Blotting, Western; D016934:Caulobacter / Q000145:classification / Q000276:immunology* / Q000302:isolation & purification; D004751:Enteritis / Q000382:microbiology; D004755:Enterobacteriaceae / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology; D007132:Immunoglobulin Isotypes / Q000276:immunology; D007147:Immunoglobulin Light Chains / Q000276:immunology; D007145:Immunoglobulin kappa-Chains; D008070:Lipopolysaccharides / Q000276:immunology*; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D007132:Immunoglobulin Isotypes; D007147:Immunoglobulin Light Chains; D007145:Immunoglobulin kappa-Chains; D008070:Lipopolysaccharides