title: Effect of dietary linoleic acid content on the distribution of triacylglycerol molecular species in rat adipose tissue.
abstract: The present study examined the effect of varying dietary linoleate intake (0.01, 0.24, 2.4, 24, 80 or 160 g/kg diet) for 24 weeks on the distribution of triacylglycerol (TG) molecular species in rat epididymal adipose tissue. Adipose TG fractions were purified by thin-layer chromatography and separated into different molecular species by reverse-phase high-performance liquid chromatography. The identification of TG species was based on fatty acid composition, retention time and the theoretical carbon number. When the dietary 18:2n-6 content was equal to or less than 24 g/kg, no significant amounts of n-6 fatty acids (mainly 18:2n-6) were observed in adipose tissue TG despite the fact that the levels of 20:4n-6 in liver phospholipids increased significantly. There were 12 major molecular species in adipose tissue when the dietary 18:2n-6 content was less than 2.4 g/kg. When the dietary 18:2n-6 content reached 24 g/kg, an additional six TG species containing one, two or three molecules of 18:2n-6 were observed. The levels of TG molecules containing two or three 18:2n-6 residues were further increased when the diet contained very large amounts of linoleic acid (160 g/kg). Conversely, those TG species containing only one 18:2n-6 residue became less abundant. It is suggested that the accumulation of these linoleate-rich TG molecular species in adipose tissue, particularly di- and trilinoleoyl containing TG, is the result of an adequate or an excessive intake of linoleic acid.
authors: Efamol Research Institute, Kentville, Nova Scotia, Canada.
authors: Y S
authors: YS
authors: Huang
authors: X
authors: X
authors: Lin
authors: R S
authors: RS
authors: Smith
authors: P R
authors: PR
authors: Redden
authors: D K
authors: DK
authors: Jenkins
authors: D F
authors: DF
authors: Horrobin
mesh_terms: D000273:Adipose Tissue / Q000187:drug effects*; D000818:Animals; D000833:Animals, Suckling; D004041:Dietary Fats / Q000494:pharmacology*; D004822:Epididymis / Q000737:chemistry; D005227:Fatty Acids / Q000032:analysis*; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000494:pharmacology*; D008099:Liver / Q000737:chemistry; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D014018:Tissue Distribution; D014280:Triglycerides / Q000737:chemistry*; D014886:Weaning
chemical_list: D004041:Dietary Fats; D005227:Fatty Acids; D008041:Linoleic Acids; D014280:Triglycerides; D019787:Linoleic Acid