Circular dichroic evidence for regulation of enzymatic activity by nonsubstrate hydrophobic ligand on glutathione S-transferase P.
1-Anilinonaphthalene-8-sulfonic acid (ANS) noncompetitively inhibited enzyme activity of glutathione S-transferase P for both glutathione and 1-chloro-2,4-dinitrobenzene (Ki = 30 microM). Dissociation constant for ANS.GST-P complex calculated from the binding study was 15 microM. From the similar values of the inhibition constant and the dissociation constant, it was concluded that specific ANS binding caused the loss of enzyme activity. In the protein structural analysis by circular dichroism, the secondary structures remarkably changed by ANS binding in accordance with the decrease of enzymatic activities. The conformational change of the protein and the decrease in enzymatic activity were reversed by dissociation of ANS. This fact strongly suggested that the enzymatic activity was regulated by a nonsubstrate hydrophobic ligand.
Nishihira
J
J
Department of Biochemistry, School of Medicine, Hokkaido University, Sapporo, Japan.
Ishibashi
T
T
Sakai
M
M
Nishi
S
S
Kumazaki
T
T
Hatanaka
Y
Y
D000817:Anilino Naphthalenesulfonates / Q000378:metabolism / Q000494:pharmacology*; D002942:Circular Dichroism; D004926:Escherichia coli / Q000235:genetics; D005456:Fluorescent Dyes; D005982:Glutathione Transferase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D007700:Kinetics; D008024:Ligands; D011485:Protein Binding; D017433:Protein Structure, Secondary / Q000187:drug effects*; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D013056:Spectrophotometry, Ultraviolet
D000817:Anilino Naphthalenesulfonates; D005456:Fluorescent Dyes; D008024:Ligands; D011994:Recombinant Proteins; C027132:1-anilino-8-naphthalenesulfonate; D005982:Glutathione Transferase