Mouse microtubule-associated protein 2 expression in transgenic Drosophila.
Full-length mouse microtubule-associated protein 2 complementary DNA inserted between Drosophila heat-shock protein 70 promoter and trailer was introduced in the germ-line of Drosophila melanogaster by P element-mediated transformation. Three transformant lines contained microtubule-associated protein 2 complementary DNA in sense orientation and two in antisense orientation. All sense lines produced microtubule-associated protein 2 after heat-shock in several tissues at low level. In the adult brain, high-level microtubule associated protein 2 expression independent of heat induction occurred in specific neuron types in each sense transformant, suggesting the action of neuron-specific enhancer genes. High microtubule-associated protein 2 levels are expected to interfere with the cells' activity by forming unphysiological cross-links, which may help elucidate the function of the given neurons.
Adám
G
G
Institute of Enzymology, Hungarian Academy of Sciences, Budapest.
Udvardy
A
A
Friedrich
P
P
D000818:Animals; D030801:Animals, Genetically Modified; D001345:Autoradiography; D004247:DNA / Q000235:genetics*; D004331:Drosophila melanogaster / Q000378:metabolism*; D008715:Methionine / Q000378:metabolism; D051379:Mice; D008869:Microtubule-Associated Proteins / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics*; D009420:Nervous System / Q000166:cytology / Q000378:metabolism; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D013462:Sulfur Radioisotopes
D008869:Microtubule-Associated Proteins; D012333:RNA, Messenger; D013462:Sulfur Radioisotopes; D004247:DNA; D008715:Methionine