Automated high-performance liquid chromatographic determination of chloramphenicol in milk and swine muscle tissue using on-line immunoaffinity sample clean-up.
An on-line high-performance liquid immunoaffinity chromatographic (HPLIAC) system for the direct determination of chloramphenicol in milk and swine muscle tissue is described. The system consisted of a dual-column system in which an HPLIAC column was directly coupled to an RP-8 high-performance liquid chromatographic column. Skimmed and deproteinated milk or aqueous muscle tissue extract was directly injected into the HPLIAC column. After a washing step with phosphate-buffered saline, chloramphenicol was desorbed by a glycine-NaCl buffer (pH 2.8) and directly concentrated on the RP-8 column. Next, chromatography was carried out using acetonitrile-sodium acetate buffer as the mobile phase. Chloramphenicol was detected at 280 nm. Mean recoveries from spiked raw milk were 70 +/- 2% (1-50 micrograms/kg) and from spiked swine muscle tissue 64 +/- 2% (10-50 micrograms/kg). The calibration curves were linear in the range 1-200 micrograms/kg spiking levels. Limits of determination were 1 microgram/kg for milk and 10 micrograms/kg for muscle tissue.
Moretti
V M
VM
Department of the Science of Food of Animal Origin, Faculty of Veterinary Medicine, University of Utrecht, Netherlands.
van de Water
C
C
Haagsma
N
N
D000818:Animals; D001322:Autoanalysis; D002701:Chloramphenicol / Q000032:analysis*; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D007118:Immunoassay; D007202:Indicators and Reagents; D008892:Milk / Q000737:chemistry*; D009132:Muscles / Q000737:chemistry*; D013552:Swine; D014020:Tissue Extracts / Q000032:analysis
D007202:Indicators and Reagents; D014020:Tissue Extracts; D002701:Chloramphenicol