title: Liquid phase enzyme reactor, a new bioreactor principle.
abstract: One of the drawbacks of solid phase enzyme reactors is the sharp decrease in substrate affinity as shown by an increase in the Km app value. To circumvent this problem a liquid phase enzyme reactor system was designed. Biotin labeled L-Tryptophan side chain oxidase (TSO) was directly injected into the plasma circuit of a filter plasmaphoresis system of rabbits and adsorbed onto an Avidin column prior to its reentry into the animal's general blood circulation. In five experiments total L-Tryptophan depletion could be observed in the plasma circuit during 60 minutes of the experiment with complete readsorption of the enzyme to the Avidin column.
authors: Department of Laboratory Medicine, University of Washington, Seattle.
authors: G
authors: G
authors: Schmer
mesh_terms: D000042:Absorption; D000818:Animals; D001360:Avidin / Q000737:chemistry; D001710:Biotin / Q000737:chemistry*; D006899:Mixed Function Oxygenases / Q000378:metabolism*; D010956:Plasmapheresis; D011817:Rabbits; D014364:Tryptophan / Q000097:blood*
chemical_list: D001360:Avidin; D001710:Biotin; D014364:Tryptophan; D006899:Mixed Function Oxygenases; C021442:indolyl-3-alkane-alpha-hydroxylase