Cystatin C in the human pancreas and gut: an immunohistochemical study of normal and neoplastic tissues.
The occurrence of immunoreactive cystatin C (CC) in normal and neoplastic cells of the human pancreas and gut was investigated using an indirect streptavidin-biotin method on formaldehyde-fixed and paraffin-embedded tissues. Virtually all pancreatic islet cells and many neuroendocrine cells throughout the gastrointestinal tract showed strong CC immunoreactivity and a granular cytoplasmic staining pattern. All 14 endocrine pancreatic tumours (insulinomas, glucagonomas, gastrinomas and non-producing tumours), as well as 16 of 17 gut carcinoid tumours, were also strongly CC immunoreactive. In addition, non-endocrine epithelial cells of pancreatic ducts and the gastrointestinal mucosa and 20 of the 24 adenocarcinomas from these sites showed weak CC immunoreactivity. Thus, CC cannot be used as a reliable immunohistochemical marker for endocrine gastro-entero-pancreatic tumours despite the fact that the protein is strongly expressed in a majority of such tumours.
Lignelid
H
H
Department of Clinical Chemistry and Pathology, Danderyd Hospital, Sweden.
Jacobsson
B
B
D000236:Adenoma / Q000378:metabolism; D002864:Chromogranins / Q000378:metabolism; D055316:Cystatin C; D015891:Cystatins / Q000032:analysis*; D005753:Gastric Mucosa / Q000378:metabolism*; D005935:Glucagonoma / Q000378:metabolism*; D006801:Humans; D007340:Insulinoma / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000378:metabolism*; D010179:Pancreas / Q000378:metabolism*; D010190:Pancreatic Neoplasms / Q000378:metabolism*; D011228:Prealbumin / Q000032:analysis / Q000378:metabolism
C525030:CST3 protein, human; D002864:Chromogranins; D055316:Cystatin C; D015891:Cystatins; D011228:Prealbumin