The PR264/c-myb connection: expression of a splicing factor modulated by a nuclear protooncogene.
We have previously reported that expression of the c-myb gene in normal avian thymic cells proceeds through the intermolecular recombination of ET (thymus-specific) and c-myb coding sequences, thereby generating a novel type of c-myb product. Antisense transcripts expressed from the ET locus encode the extremely well-conserved splicing factor PR264/SC35. We now show that the human PR264 promoter sequences contain several myb-recognition elements that efficiently interact in vitro with the c-myb DNA-binding domain. Moreover, expression from the PR264 promoter is transactivated, both in vitro and in cultured cells, by different c-myb products. Thus, the PR264 gene is most likely a physiological target for the c-myb family of transcription factors.
Sureau
A
A
Laboratoire d'Oncologie Virale et Moléculaire, Institut Curie, Centre Universitaire, Orsay, France.
Soret
J
J
Vellard
M
M
Crochet
J
J
Perbal
B
B
D000818:Animals; D001483:Base Sequence; D002645:Chickens; D004268:DNA-Binding Proteins / Q000378:metabolism / Q000502:physiology; D005786:Gene Expression Regulation; D006367:HeLa Cells; D006410:Hematopoiesis; D006801:Humans; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics*; D011401:Promoter Regions, Genetic; D011518:Proto-Oncogene Proteins / Q000502:physiology*; D020598:Proto-Oncogene Proteins c-myb; D012326:RNA Splicing; D012333:RNA, Messenger / Q000235:genetics; D015183:Restriction Mapping; D012261:Ribonucleoproteins / Q000235:genetics*; D000068103:Serine-Arginine Splicing Factors; D014157:Transcription Factors / Q000502:physiology; D015533:Transcriptional Activation
D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D011518:Proto-Oncogene Proteins; D020598:Proto-Oncogene Proteins c-myb; D012333:RNA, Messenger; D012261:Ribonucleoproteins; D014157:Transcription Factors; C073288:SRSF2 protein, human; D000068103:Serine-Arginine Splicing Factors