Increased internal calcium mobilization in platelets of patients with chronic renal failure.
Platelet free calcium concentrations ([Ca2+]i) were measured with Fura-2 to elucidate the intracellular calcium kinetics in patients with renal disease. There were no significant differences of the resting [Ca2+]i among the control subjects (C) (n = 12), patients with chronic glomerulonephritis (CGN) (n = 8), and patients with chronic renal failure (CRF) (n = 12). In all groups, platelets [Ca2+]i were significantly increased by agonists (thrombin, adenosine diphosphate) compared with their respective basal level. Thrombin-induced [Ca2+]i rise was significantly higher in CRF (840 +/- 265 nM) than in C (600 +/- 163) and CGN (562 +/- 137). Also adenosine diphosphate elicited similar responses. In the presence of calcium chelator in the incubation buffer, the elevation of [Ca2+]i after thrombin stimulation was statistically higher in CRF (469 +/- 85 nM) than in C (275 +/- 60) and CGN (301 +/- 41). These findings suggest that platelets of CRF were capable of increasing [Ca2+]i in response to agonists, through further mobilization of calcium from the intracellular pool rather than the elevation of transmembrane calcium influx.
Tokumoto
A
A
Second Department of Internal Medicine, Tottori University School of Medicine, Yonago, Japan.
Uemasu
J
J
Kawasaki
H
H
D000244:Adenosine Diphosphate / Q000494:pharmacology; D000328:Adult; D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism*; D002118:Calcium / Q000037:antagonists & inhibitors / Q000097:blood*; D005707:Gallic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D005921:Glomerulonephritis / Q000097:blood; D006801:Humans; D007676:Kidney Failure, Chronic / Q000097:blood*; D008875:Middle Aged; D013917:Thrombin / Q000494:pharmacology
C006014:8-(N,N-diethylamino)octyl-3,4,5-trimethoxybenzoate; D000244:Adenosine Diphosphate; D005707:Gallic Acid; D013917:Thrombin; D002118:Calcium