Gas-liquid chromatographic analysis of volatile short chain fatty acids in fecal samples as pentafluorobenzyl esters.
A protocol was developed for the analysis of volatile short chain fatty acids in microsamples of feces, short chain fatty acid (SCFA) extraction was from fecal samples using ethanol incorporating n-hexanoic acid as an internal standard. The SCFAs were converted to pentafluorobenzyl esters with alpha-2,3,4,5,6-pentafluorotoluene and analyzed on a gas-liquid chromatograph equipped with an electron capture detector. One hundred milligrams of sample was routinely used but analysis could be carried out on 20 mg of sample.
McGrath
L T
LT
Department of Therapeutics and Pharmacology, Queen's University of Belfast, Northern Ireland, United Kingdom.
Weir
C D
CD
Maynard
S
S
Rowlands
B J
BJ
D000818:Animals; D001565:Benzoates; D002208:Caproates; D002849:Chromatography, Gas / Q000379:methods; D004952:Esters; D005230:Fatty Acids, Nonesterified / Q000032:analysis*; D005243:Feces / Q000737:chemistry*; D007202:Indicators and Reagents; D008297:Male; D051381:Rats; D017208:Rats, Wistar
D001565:Benzoates; D002208:Caproates; D004952:Esters; D005230:Fatty Acids, Nonesterified; D007202:Indicators and Reagents; C037652:hexanoic acid