title: Induction of an increased number of dendritic cells in the peritoneal cavity of rats by intraperitoneal administration of Bacillus Calmette-Guérin.
abstract: Recently we described the presence of a small number of DC among the peritoneal cells of steady state rats. These DC had the same morphological characteristics and a similar antigen-presenting capacity as DC isolated from the spleen. This study shows that in the peritoneal cavity, which is a non-lymphoid microenvironment, the number of DC increases after i.p. administration of BCG. Next to this relatively small influx of DC, the approximately three-fold increase of the total number of cells is predominantly caused by an enormous influx of neutrophilic granulocytes, and to a lesser extent by an influx of macrophages. The phenotype and the antigen-presenting capacity of peritoneal DC has not changed, while the number of Ia-positive M phi has increased. Nevertheless, due to a suppressive effect of the peritoneal M phi, the total peritoneal cell suspension is no longer capable of presenting antigen.
authors: Department of Cell Biology, Medical Faculty, Vrije Universiteit, Amsterdam, The Netherlands.
authors: E
authors: E
authors: van Vugt
authors: M A
authors: MA
authors: Verdaasdonk
authors: R H
authors: RH
authors: Beelen
authors: E W
authors: EW
authors: Kamperdijk
mesh_terms: D000818:Animals; D000938:Antigen-Presenting Cells; D001500:BCG Vaccine / Q000276:immunology*; D003713:Dendritic Cells / Q000276:immunology* / Q000648:ultrastructure; D016130:Immunophenotyping; D007166:Immunosuppressive Agents / Q000276:immunology; D036341:Intercellular Signaling Peptides and Proteins; D008297:Male; D008854:Microscopy, Electron; D010455:Peptides / Q000276:immunology; D010529:Peritoneal Cavity / Q000166:cytology*; D051381:Rats; D011912:Rats, Inbred ACI; D013154:Spleen / Q000166:cytology
chemical_list: D001500:BCG Vaccine; D007166:Immunosuppressive Agents; D036341:Intercellular Signaling Peptides and Proteins; D010455:Peptides; C027606:L-glutamic acid-L-tyrosine copolymer