Separation of dolichol from dehydrodolichol by a simple two-plate thin-layer chromatography.
A novel thin-layer chromatographic procedure was devised to separate dolichol and dehydrodolichol from each other with the concomitant separation of each family with respect to the carbon chain length. This method involves development of the polyprenols successively on two different plates, a silica gel plate and a reversed-phase plate.
Sagami
H
H
Institute for Chemical Reaction Science, Tohoku University, Sendai, Japan.
Kurisaki
A
A
Ogura
K
K
Chojnacki
T
T
D000818:Animals; D002855:Chromatography, Thin Layer / Q000295:instrumentation / Q000379:methods*; D004288:Dolichol Phosphates / Q000378:metabolism; D004286:Dolichols / Q000031:analogs & derivatives* / Q000302:isolation & purification* / Q000378:metabolism; D005069:Evaluation Studies as Topic; D066298:In Vitro Techniques; D008099:Liver / Q000737:chemistry / Q000378:metabolism; D008297:Male; D010944:Plants / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D013552:Swine
D004288:Dolichol Phosphates; D004286:Dolichols; C079181:dehydrodolichol