Isolation and amino acid sequence of the TRH-potentiating peptide from bovine hypothalamus.
A neuropeptide termed TRH-potentiating peptide, which potentiates TRH-evoked thyrotropin secretion by antehypophysis in vitro, was isolated from an acetonic powder of bovine hypothalamus. The peptide was purified to homogeneity by a 3-step protocol involving molecular sieve filtration, ion-exchange chromatography and reverse phase high performance liquid chromatography. The complete amino acid sequence of the decapeptide was determined as Ser-Phe-Pro-Trp-Met-Glu-Ser-Asp-Val-Thr by automated Edman degradation with a solid-phase sequencer. Bovine TRH-potentiating peptide is structurally identical to Ps4, a decapeptide which was deduced from the cDNA encoding the rat TRH precursor. This study provides for the first time a direct chemical evidence for the existence of non-TRH peptides originating from posttranslational processing of the TRH precursor in vivo.
Bulant
M
M
Laboratoire de Bioactivation des Peptides, Institut Jacques Monod, Université Paris 7, France.
Ladram
A
A
Montagne
J J
JJ
Delfour
A
A
Nicolas
P
P
D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D007031:Hypothalamus / Q000737:chemistry*; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000302:isolation & purification*; D011498:Protein Precursors / Q000737:chemistry / Q000302:isolation & purification*; D013973:Thyrotropin-Releasing Hormone / Q000737:chemistry / Q000302:isolation & purification*
D010446:Peptide Fragments; D011498:Protein Precursors; C057639:prepro-thyrotropin releasing hormone (160-169); D013973:Thyrotropin-Releasing Hormone