title: Subculturing of a polychlorinated biphenyl-dechlorinating anaerobic enrichment on solid media.
abstract: An anaerobic culture capable of dechlorinating polychlorinated biphenyls was subcultured under strict anaerobic conditions on solid media containing sterilized river sediment. The dechlorination activity was transferred as a bacterial colony on a solid medium three times. After two transfers on solid medium, the culture was no longer methanogenic but still dechlorinated a mixture of tri- and tetrachlorobiphenyls. This demonstrates that anaerobic bacteria are responsible for the polychlorinated biphenyl dechlorination and can be grown without polychlorinated biphenyl on solid media.
authors: Celgene Corp., Warren, New Jersey 07059.
authors: H D
authors: HD
authors: May
authors: A W
authors: AW
authors: Boyle
authors: W A
authors: WA
authors: Price
authors: C K
authors: CK
authors: Blake
mesh_terms: D001421:Bacteria, Anaerobic / Q000254:growth & development* / Q000378:metabolism*; D001431:Bacteriological Techniques; D001673:Biodegradation, Environmental; D003470:Culture Media; D004785:Environmental Pollutants / Q000378:metabolism; D005069:Evaluation Studies as Topic; D011078:Polychlorinated Biphenyls / Q000378:metabolism*
chemical_list: D003470:Culture Media; D004785:Environmental Pollutants; D011078:Polychlorinated Biphenyls