Influence of kinetin (6-furfurylo-amino-purine) on human fibroblasts in the cell culture.
Influence of kinetin on human fibroblasts cultured in vitro was studied. Investigations were carried out on I passage fibroblasts, taken from the human skin. Feulgen's method and radioautography were used. Quantitative estimations of DNA in nuclei of fibroblasts were done on cytophotometer. An increase in amount of DNA in nuclei of fibroblasts cytophotometer. An increase in amount of DNA in nuclei of fibroblasts cultured in the presence of kinetin was found. Stronger 3H uridine incorporation was recorded in cultures after administration of the lowest dosis of kinetin. Kinetin, derivative of adenine (6-furfurylo-amino-purine) belongs to cytokinins and it regulates the growth of plants.
Kowalska
E
E
Department of Biology, Academy of Medicine, Szczecin.
D000225:Adenine / Q000031:analogs & derivatives* / Q000494:pharmacology; D002478:Cells, Cultured; D005260:Female; D005347:Fibroblasts / Q000187:drug effects*; D006801:Humans; D007701:Kinetin; D010937:Plant Growth Regulators / Q000494:pharmacology*
D010937:Plant Growth Regulators; D000225:Adenine; D007701:Kinetin