Identification of the cellulose-binding domain of the cellulosome subunit S1 from Clostridium thermocellum YS.
The 3' region of a gene designated cipB, which shows strong homology with cipA that encodes the cellulosome SL subunit of Clostridium thermocellum ATCC 27405, was isolated from a gene library of C. thermocellum strain YS. The truncated S1 protein encoded by the cipB derivative bound tightly to cellulose. The cellulose-binding domain in this polypeptide consisted of a C-terminal proximal 167 residue sequence which showed complete identity with residues 337-503 of mature SL from C. thermocellum strain ATCC 27405. The cellulose-binding domain interacted with both crystalline and amorphous cellulose, but not with xylan.
Poole
D M
DM
Department of Biological Science, University of Newcastle upon Tyne, UK.
Morag
E
E
Lamed
R
R
Bayer
E A
EA
Hazlewood
G P
GP
Gilbert
H J
HJ
D000595:Amino Acid Sequence; D001483:Base Sequence; D001665:Binding Sites; D002352:Carrier Proteins / Q000235:genetics*; D002480:Cellulase / Q000235:genetics*; D002482:Cellulose / Q000378:metabolism*; D003013:Clostridium / Q000235:genetics* / Q000378:metabolism*; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000235:genetics*; D015183:Restriction Mapping
D002352:Carrier Proteins; D004269:DNA, Bacterial; D009097:Multienzyme Complexes; D002482:Cellulose; D002480:Cellulase