Free radical injury in skin cultured fibroblasts from Alzheimer's disease patients.
Oxygen radical production is postulated to be a major cause of cell damage in aging. We have studied the response to toxic oxygen metabolites of fibroblast cell lines derived from skin biopsies of patients with familial and sporadic Alzheimer's disease compared with those derived from normal controls. Fibroblasts were damaged by the generation of oxygen metabolites during the enzymatic oxidation of acetaldehyde by 50 mU of xanthine-oxidase. To quantify cell damage we measured lactate dehydrogenase activity in the culture medium and cell viability in fibroblast cultures from four normal subjects, five FAD, and four AD patients after 2 hours of Xo incubation. We found a significant increase of LDH activity in FAD vs. controls and also in AD vs. controls, suggesting that AD cells are more susceptible to oxygen radical damage than are normal controls.
Tesco
G
G
Department of Neurology, University of Florence, Italy.
Latorraca
S
S
Piersanti
P
P
Sorbi
S
S
Piacentini
S
S
Amaducci
L
L
D000544:Alzheimer Disease / Q000201:enzymology / Q000473:pathology*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D005347:Fibroblasts / Q000187:drug effects / Q000201:enzymology / Q000473:pathology; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000494:pharmacology*; D012867:Skin / Q000187:drug effects* / Q000201:enzymology / Q000473:pathology
D017382:Reactive Oxygen Species; D007770:L-Lactate Dehydrogenase