title: Effects of oxygen concentration on the metabolism of anisole homologues by rat liver microsomes.
abstract: 1. The effects of oxygen concentration were studied on the metabolic pathways of anisole homologues (anisole, phenetole and isopropoxybenzene) catalysed by liver microsomes from phenobarbital-treated rats. 2. With increase of oxygen concentration, the rate of anisole o-hydroxylation reached a plateau at about 35 microM O2, while the rates of O-demethylation and aromatic p-hydroxylation were still increasing at 223 microM O2 (air). 3. The rates of all three metabolic reactions of phenetole reached plateau levels at about 80 microM O2. 4. The rates of all three metabolic reactions of iso-propoxybenzene were still increasing as 223 microM O2 (air). 5. The ratio of aromatic p-hydroxylation or O-dealkylation to aromatic o-hydroxylation decreased in anisole metabolism, and showed no uniform change in phenetole and isopropoxybenzene metabolism with decreasing oxygen concentration. 6. The ratio of aromatic p-hydroxylation to O-dealkylation was essentially constant over the range of oxygen concentration studied in anisole and phenetole metabolism, while in iso-propoxybenzene metabolism the ratio was different between higher and lower oxygen concentrations than 60 microM. 7. This series of compounds with increasing chain length did not show homologous changes in rates of product formation or O2 dependent of product formation.
authors: Faculty of Pharmaceutical Sciences, University of Tokyo, Japan.
authors: H
authors: H
authors: Ohi
authors: E
authors: E
authors: Takahara
authors: S
authors: S
authors: Ohta
authors: M
authors: M
authors: Hirobe
mesh_terms: D000818:Animals; D000840:Anisoles / Q000378:metabolism*; D001571:Benzoflavones / Q000494:pharmacology; D066298:In Vitro Techniques; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism*; D010100:Oxygen / Q000494:pharmacology*; D010634:Phenobarbital / Q000494:pharmacology; D010647:Phenyl Ethers / Q000378:metabolism; D011335:Proadifen / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000840:Anisoles; D001571:Benzoflavones; D010647:Phenyl Ethers; C079412:isopropyl phenyl ether; C011512:alpha-naphthoflavone; D011335:Proadifen; C079413:phenetole; D010100:Oxygen; D010634:Phenobarbital