Basic conditions for the drug selection and transient gene expression in the cultured cell line of Bombyx mori.
We established basic conditions for transient gene expression and selection of antibiotics in the cultured cell line of silkworm, Bombyx mori, by use of the promoter of the heat shock protein (hsp70) gene of Drosophila melanogaster. The control promoter (hsp70) promoted the expression of chloramphenicol acetyltransferase (CAT) gene ligated at the downstream, dependent on the orientation of the promoter in the silkworm cell. The cell line is able to be supplied for the promoter assay of the silkworm genes. The concentration for the drug selection was determined as 0.75 mg/ml on neomycin analog, G418 (geneticin).
Okano
K
K
Department of Agrobiology, Faculty of Agriculture, University of Tokyo, Japan.
Miyajima
N
N
Takada
N
N
Kobayashi
M
M
Maekawa
H
H
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D012831:Bombyx / Q000166:cytology* / Q000235:genetics; D002460:Cell Line; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D004331:Drosophila melanogaster / Q000737:chemistry; D015870:Gene Expression / Q000187:drug effects* / Q000235:genetics; D005839:Gentamicins / Q000494:pharmacology; D006360:Heat-Shock Proteins / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics
D000900:Anti-Bacterial Agents; D005839:Gentamicins; D006360:Heat-Shock Proteins; C010680:antibiotic G 418; D015500:Chloramphenicol O-Acetyltransferase