Blood cultures from Bangladeshi children with septicaemia: an evaluation of conventional, lysis-direct plating and lysis-centrifugation methods.
The use of a laboratory-made lysis-direct plating and lysis-centrifugation (LDP/LC) device for blood cultivation has been compared with the conventional broth culture method in respect of speed and sensitivity in detecting organism(s) and cost effectiveness. 400 blood cultures yielded 95 clinically significant isolates. Both methods recovered 73 organisms (76.8%); 20 (21%) were detected by LDP/LC methods only, and 2 (2.1%) were isolated by the conventional method only. All the 93 isolates (97.8%) recovered by LDP/LC were isolated within 48 h, whereas the broth culture method took 7 d to isolate a total of 75 organisms (78.9%). The LDP/LC method, with our laboratory-made device, costs one-fourth of the cost of the conventional broth culture system.
Saha
S K
SK
Department of Microbiology, Dhaka Shishu (Children) Hospital, Bangladesh.
Khan
W A
WA
Saha
S
S
D001431:Bacteriological Techniques; D001433:Bacteriolysis; D002498:Centrifugation; D002648:Child; D002675:Child, Preschool; D006801:Humans; D018805:Sepsis / Q000097:blood* / Q000209:etiology