[Plasmid DNA transfection in fibroblasts of athymic rats].
Nontumorigenic clone FR-7 cl 13 from fibroblasts of athymic rat was obtained from stroma of human colon carcinoma xenograft propagated on nude animals. Spontaneous transformation of this cells was absent after 40 passages in vitro and treatment with pSV2neo. But cells give rise to tumors in athymic mice after transfection with pEJ. This cell clone can be recommended as cells-targets for transfection.
Bryzgalov
I P
IP
Galetskiĭ
S A
SA
Nikiforov
V G
VG
Revazova
E S
ES
D000818:Animals; D002417:Cattle; D002460:Cell Line; D003110:Colonic Neoplasms; D004247:DNA / Q000235:genetics*; D005347:Fibroblasts; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D010957:Plasmids / Q000235:genetics*; D051381:Rats; D011923:Rats, Nude; D014162:Transfection; D014183:Transplantation, Heterologous
D004247:DNA
Issledovanie transfektsii plazmidnoĭ DNK v fibroblasty bestimusnoĭ krysy.