Changes in the xanthine dehydrogenase/xanthine oxidase ratio in the rat kidney subjected to ischemia-reperfusion stress: preventive effect of some flavonoids.
The enzyme xanthine oxidase has been implicated in the tissue oxidative injury after ischemia-reperfusion. This enzyme, which is a source of oxygen free radicals, is formed from a dehydrogenase form during ischemia. The ratio dehydrogenase/oxidase of rat kidney homogenates decreases during the ischemia and the reperfusion. Two flavonoids, quercetin and silybin, characterized as free radical scavengers, exert a protective effect preventing the decrease in the dehydrogenase/oxidase ratio observed during ischemia-reperfusion. The mechanism of this effect and the role of flavonoids in the ischemia-reperfusion tissue damage is discussed.
Sanhueza
J
J
Unidad de Bioquimica Farmacologica y Lipidos, INTA, Universidad de Chile, Santiago.
Valdes
J
J
Campos
R
R
Garrido
A
A
Valenzuela
A
A
D000818:Animals; D016166:Free Radical Scavengers; D007511:Ischemia / Q000201:enzymology / Q000517:prevention & control*; D007668:Kidney / Q000098:blood supply* / Q000201:enzymology*; D008297:Male; D011794:Quercetin / Q000627:therapeutic use; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000517:prevention & control*; D012838:Silymarin / Q000627:therapeutic use; D014527:Uric Acid / Q000378:metabolism; D014968:Xanthine Dehydrogenase / Q000378:metabolism*; D014969:Xanthine Oxidase / Q000378:metabolism*
D016166:Free Radical Scavengers; D012838:Silymarin; D014527:Uric Acid; D011794:Quercetin; D014968:Xanthine Dehydrogenase; D014969:Xanthine Oxidase