[Proliferation kinetic studies in the syngeneic liver transplantation mode in the rat. Effect of re-arterialization on the transplant].
Successful orthotopic liver transplantation (OLT) can be achieved in the rat. We used bromo-2-deoxyuridine (BrdU) as a proliferation marker to document morphological differences between OLT with and without rearterialization. Animals with portal anastomosis alone had a significantly increased proliferation rate of hepatocytes, Kupffer cells, and bile duct epithelial cells, as indicated by strong staining with BrdU, 8 days post-transplant compared to animals with rearterialization. Regeneration of ischemically damaged liver parenchymal cells may account for this observation. Thus, OLT with rearterialization appears to be the more physiological transplant model.
Hanisch
E
E
Zentrum der Chirurgie, Johann-Wolfgang-Goethe-Universität, Frankfurt/Main.
Herrmann
G
G
Lemperle
S
S
Jakobi
R
R
Weber
T
T
Wagner
M
M
D000714:Anastomosis, Surgical / Q000379:methods*; D000818:Animals; D001011:Aorta / Q000473:pathology / Q000601:surgery; D002455:Cell Division / Q000502:physiology*; D006085:Graft Survival / Q000502:physiology; D006499:Hepatic Artery / Q000473:pathology / Q000601:surgery; D007124:Immunoenzyme Techniques; D008099:Liver / Q000098:blood supply* / Q000473:pathology; D008115:Liver Regeneration / Q000502:physiology*; D016031:Liver Transplantation / Q000379:methods* / Q000473:pathology; D008297:Male; D008866:Microsurgery / Q000379:methods*; D051381:Rats; D011917:Rats, Inbred Lew; D016196:S Phase / Q000502:physiology
Proliferationskinetische Untersuchungen im syngenen Lebertransplantationsmodell der Ratte. Einfluss der Rearterialisation auf das Transplantat.