Continuous flow contact lens delivery of gentamicin to rabbit cornea and aqueous humor.
The Morgan Therapeutic Lens (MTL) was investigated as a continuous corneal perfusion system in New Zealand white rabbits. Gentamicin concentration in the cornea and aqueous humor delivered by the MTL was compared to gentamicin drops (13.6 mg/ml) administered every 15 or 30 minutes. Gentamicin (1 mg/ml) or 5 mg/ml was perfused at 10 ml/hr for up to 4.0 hours. At each time interval of 0.5, 1, 2 and 4 hours, homogenized corneas and aqueous humour were assayed for gentamicin concentrations. The highest aqueous humour gentamicin concentrations of 57.99 +/- 12.86 micrograms/ml were significantly higher with the MTL and 5 mg/ml of gentamicin compared with the MTL and 1 mg/ml of gentamicin or than drops applied every 30 minutes, but not significantly different than drops every 15 minutes. Highest corneal concentrations of gentamicin of 496.04 +/- 101.16 micrograms/gm cornea were significantly higher with MTL and 5 mg/ml of gentamicin compared with the MTL and 1 mg/ml gentamicin or than drops applied every 30 minutes, but not significantly different than drops every 15 minutes. All mean gentamicin concentrations attained via the MTL exceeded the mean inhibitory concentration for most sensitive bacterial species. The MTL is a reliable drug delivery system with distinct advantages, and may be a useful therapeutic modality in the ocular delivery of gentamicin and other drugs.
Rootman
D S
DS
Department of Ophthalmology, University of Toronto, Ontario, Canada.
Willoughby
R P
RP
Bindlish
R
R
Avaria
M
M
Basu
P K
PK
Krajden
M
M
D000818:Animals; D001082:Aqueous Humor / Q000378:metabolism*; D003261:Contact Lenses; D003315:Cornea / Q000378:metabolism*; D016503:Drug Delivery Systems; D005839:Gentamicins / Q000008:administration & dosage / Q000493:pharmacokinetics*; D009883:Ophthalmic Solutions; D011817:Rabbits
D005839:Gentamicins; D009883:Ophthalmic Solutions