Modifying effects of fungal and herb metabolites on azoxymethane-induced intestinal carcinogenesis in rats.
Modifying effects of a fungal product, flavoglaucin, and four plant-derived chemicals, shikonin, gingerol, oleanolic acid and paeoniflorin, on intestinal carcinogenesis were examined in a rat model using azoxymethane (AOM). A total of 280 male F344 rats, 6 weeks old, were divided into 12 groups. Group 1 (30 rats) was given two subcutaneous injections of 15 mg/kg of AOM at the start of the experiment. Groups 2 (30 rats), 3 (20 rats), 4 (20 rats), 5 (30 rats) and 6 (30 rats) received a test chemical (flavoglaucin, shikonin, gingerol, oleanolic acid or paeoniflorin, respectively) in the diet at a concentration of 0.02% for 3 weeks, during which time AOM was applied, and then kept on basal diet until the end of experiment (one year). Groups 7-11 (each 20 rats) were given a test chemical corresponding to Groups 2-6, respectively. Group 12 (20 rats) served as a control. The incidence and average number of intestinal tumors in Group 2 (47%, 0.57 +/- 0.68) were significantly less than in Group 1 (74%, 1.07 +/- 0.87) (P < 0.05, respectively). Multiplicity of intestinal neoplasms of Group 3 (0.55 +/- 0.60) or 4 (0.47 +/- 0.51) was also significantly smaller than that of Group 1 (P < 0.05 and P < 0.01, respectively). These results suggest that flavoglaucin, shikonin and gingerol might be promising chemopreventive agents for intestinal neoplasia.
Yoshimi
N
N
Department of Pathology, Gifu University School of Medicine.
Wang
A
A
Morishita
Y
Y
Tanaka
T
T
Sugie
S
S
Kawai
K
K
Yamahara
J
J
Mori
H
H
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000008:administration & dosage / Q000494:pharmacology*; D001397:Azoxymethane; D001565:Benzoates; D001952:Bridged-Ring Compounds; D002396:Catechols; D004354:Drug Screening Assays, Antitumor; D005233:Fatty Alcohols / Q000008:administration & dosage / Q000494:pharmacology*; D005841:Gentisates / Q000008:administration & dosage / Q000494:pharmacology*; D005960:Glucosides / Q000008:administration & dosage / Q000494:pharmacology*; D007414:Intestinal Neoplasms / Q000139:chemically induced / Q000517:prevention & control*; D008297:Male; D039821:Monoterpenes; D009153:Mutagens / Q000008:administration & dosage / Q000494:pharmacology*; D009285:Naphthoquinones / Q000008:administration & dosage / Q000494:pharmacology*; D009828:Oleanolic Acid / Q000008:administration & dosage / Q000494:pharmacology*; D051381:Rats; D011916:Rats, Inbred F344; D006027:Glycosides
D000972:Antineoplastic Agents, Phytogenic; D001397:Azoxymethane; D001565:Benzoates; D001952:Bridged-Ring Compounds; D002396:Catechols; D005233:Fatty Alcohols; D005841:Gentisates; D005960:Glucosides; D039821:Monoterpenes; D009153:Mutagens; D009285:Naphthoquinones; D009828:Oleanolic Acid; C015423:peoniflorin; D006027:Glycosides; C016101:shikonin; C005001:flavoglaucin; C007845:gingerol