Evaluation of Syva's enzyme immunoassay for the detection of Chlamydia trachomatis in urogenital specimens.
A newly developed microwell enzyme immunosorbent assay (EIA) system by Syva Company (Palo Alto, CA) can detect Chlamydia trachomatis in < 3 hr. It uses a polyclonal antibody to chlamydial lipopolysaccharide and end points are determined with a spectrophotometer. Three clinical trial sites (University of California Medical Center, San Francisco, CA; University of Washington, Seattle, WA; and Louisiana State University Medical Center, New Orleans, LA), compared this EIA with tissue culture (TC) for identifying Chlamydia in urogenital specimens. Overall prevalence by TC was 10.4% (136 of 1306). When tests were compared with TC (using vials or microtiter plates and a fluorescent antibody stain), we found an EIA sensitivity of 93.4% (127 of 136) and a specificity of 98.1% (1148 of 1170). This EIA has a performance profile that is, at the very least, comparable with other nonculture methods for diagnosing genital tract infections with C. trachomatis.
Moncada
J
J
Department of Laboratory Medicine, University of California, San Francisco.
Schachter
J
J
Bolan
G
G
Nathan
J
J
Shafer
M A
MA
Clark
A
A
Schwebke
J
J
Stamm
W
W
Mroczkowski
T
T
Seliborska
Z
Z
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D002584:Cervix Uteri / Q000382:microbiology; D002690:Chlamydia Infections / Q000175:diagnosis* / Q000382:microbiology; D002692:Chlamydia trachomatis / Q000276:immunology / Q000302:isolation & purification*; D005189:False Positive Reactions; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D012680:Sensitivity and Specificity; D014521:Urethra / Q000382:microbiology; D014522:Urethral Diseases / Q000175:diagnosis / Q000382:microbiology; D002577:Uterine Cervical Diseases / Q000175:diagnosis / Q000382:microbiology
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal