title: Nucleotide variation and molecular structure of the heterochromatic repetitive AluI DNA in the brine shrimp Artemia franciscana.
abstract: It has been suggested that DNA bending could play a role in the regulation of gene expression, chromosome segregation, specific recombination and/or DNA packaging. We have previously demonstrated that an AluI DNA family of repeats is the major component of constitutive heterochromatin in the brine shrimp A. franciscana. By the analysis of cloned oligomeric (monomer to hexamer) heterochromatic fragments we verified that the repetitive AluI DNA shows a stable curvature that determines a solenoidal geometry to the double helix. This particular structure could be of relevant importance in conferring the characteristic heterochromatic condensation. In this paper we evaluate how the point mutations that occurred during the evolution of the AluI sequence of A. franciscana could influence the sequence-dependent tridimensional conformation. The obtained data underline that, in spite of the high sequence mutation frequency (10%) of the repetitive DNA, the general structure of the heterochromatic DNA is not greatly influenced, but rather there is a substantial variation of the copy number of the repetitive AluI fragment. This variation could be responsible for the hypothetical function of the constitutive heterochromatin.
authors: Dipartimento di Genetica e di Biologia dei Microrganismi, Università di Milano, Italy.
authors: N
authors: N
authors: Landsberger
authors: S
authors: S
authors: Cancelli
authors: D
authors: D
authors: Carettoni
authors: C
authors: C
authors: Barigozzi
authors: G
authors: G
authors: Badaracco
mesh_terms: D000818:Animals; D001156:Artemia / Q000235:genetics*; D001483:Base Sequence; D005075:Biological Evolution; D004247:DNA / Q000737:chemistry*; D006570:Heterochromatin; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009690:Nucleic Acid Conformation; D009711:Nucleotides / Q000235:genetics*; D017354:Point Mutation; D012091:Repetitive Sequences, Nucleic Acid; D017422:Sequence Analysis, DNA
chemical_list: D006570:Heterochromatin; D009711:Nucleotides; D004247:DNA