title: Thyroidal influence on the cell surface GM1 of granule cells: its significance in cell migration during rat brain development.
abstract: 1. No difference was observed in the in vitro growing ability of granule cells isolated from hypothyroid or normal rat brain. When granule cells were taken from hypothyroid rat brain and grown in normal culture medium containing 10% fetal calf serum, they behaved similarly to the granule cells obtained from normal rat brain. 2. In both cases there were progressive losses of in vitro growing ability of the granule cells with the age of the animal and it became impossible to grow them when derived from 21 days or older animals. 3. A marked decrease in cell surface GM1 was observed when the cells were maintained under thyroid hormone-deficient conditions in culture. 4. Anti-GM1 antibody was found to inhibit significantly the migration of granule cells along the astrocyte fibers. 5. These results indicate that GM1 has an important role in thyroid hormone-dependent postnatal brain maturation in rat.
authors: Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut 06511.
authors: M
authors: M
authors: Chakraborty
authors: P
authors: P
authors: Lahiri
authors: D
authors: D
authors: Chatterjee
mesh_terms: D000367:Age Factors; D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism; D001790:Blood Physiological Phenomena; D001921:Brain / Q000254:growth & development*; D002417:Cattle / Q000097:blood; D002462:Cell Membrane / Q000737:chemistry; D002465:Cell Movement; D002478:Cells, Cultured; D002525:Cerebellar Cortex / Q000378:metabolism / Q000473:pathology*; D003470:Culture Media; D005677:G(M1) Ganglioside / Q000032:analysis / Q000502:physiology*; D007037:Hypothyroidism / Q000473:pathology*; D009474:Neurons / Q000378:metabolism / Q000473:pathology*; D051381:Rats; D013961:Thyroid Gland / Q000502:physiology*
chemical_list: D003470:Culture Media; D005677:G(M1) Ganglioside