The application of AgNOR technique on glycol-methacrylate embedded human normal and pathological tissues.
The Authors have applied the AgNOR technique to a series of normal and pathological human tissues fixed in neutral buffered formalin and methacarnoy and embedded in glycol-methacrylate. The results show the compatibility of the AgNOR technique with this embedding medium as well as the possibility to obtain an adequate visualization of the specific silver precipitates (AgNORs) on a unique focal plane without appreciable nuclear overlap, making thus the valuation of the AgNORs less subjective.
Tuccari
G
G
Dipartimento di Patologia Umana, Policlinico Universitario, Messina.
Muscarà
M
M
Giuffrè
G
G
Crisafulli
C
C
Barresi
G
G
D004064:Digestive System / Q000033:anatomy & histology; D005404:Fixatives; D006801:Humans; D008689:Methacrylates; D009369:Neoplasms / Q000473:pathology*; D009697:Nucleolus Organizer Region / Q000648:ultrastructure*; D016613:Plastic Embedding; D016622:Silver Staining; D013048:Specimen Handling; D013961:Thyroid Gland / Q000033:anatomy & histology
D005404:Fixatives; D008689:Methacrylates; C005044:hydroxyethyl methacrylate