[The establishment of human anti-tetanus toxoid hybridomas with in vitro immunized human tonsil cells].
Human tonsil cells in vitro immunized with tetanus toxoid were fused with human-mouse heteromyeloma line RF to generate human-mouse hybridomas. Hybridoma 891112-50 was cloned and 2 subclones (891112-50-3 and -4) were obtained. The secreted antibodies from the subclones were antigen specific, since they did not cross react with three irrelevant antigens (OVA, TCS and F gamma G). The hybridomas were quite stable. After 13 passages in tissue culture flasks, they still retained their antibody secreting ability. From flow cytometry analysis the subclone 50-3 was more stable than the subclone 50-4. The human immunoglobulin contained in supernatant collected during regular passages was equivalent to 69.6 micrograms/ml.
Jiang
Z Q
ZQ
Shanghai Institute of Cell Biology, Academia Sinica, China.
Sun
P F
PF
Ji
Y Y
YY
Yeh
M
M
Kuroda
K
K
Kamei
M
M
Hashizume
S
S
D000818:Animals; D000911:Antibodies, Monoclonal; D002459:Cell Fusion; D006801:Humans; D006825:Hybridomas / Q000378:metabolism*; D051379:Mice; D014066:Palatine Tonsil / Q000166:cytology / Q000276:immunology*; D013745:Tetanus Toxoid / Q000276:immunology*
D000911:Antibodies, Monoclonal; D013745:Tetanus Toxoid