Incorporation of single-stranded DNA binding protein early in polymerase chain reaction product sequencing reactions prevents enzyme pausing.
Ball
J K
JK
Regional Virus Laboratory, East Birmingham Hospital, United Kingdom.
Desselberger
U
U
D001345:Autoradiography; D001483:Base Sequence; D004268:DNA-Binding Proteins; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D004121:Dimethyl Sulfoxide; D004926:Escherichia coli; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D007202:Indicators and Reagents; D009695:Nucleic Acid Renaturation; D016133:Polymerase Chain Reaction / Q000379:methods*; D013462:Sulfur Radioisotopes; D013698:Templates, Genetic; D014759:Viral Envelope Proteins / Q000235:genetics*
D004268:DNA-Binding Proteins; D007202:Indicators and Reagents; D013462:Sulfur Radioisotopes; D014759:Viral Envelope Proteins; C034970:bacteriophage T7 induced DNA polymerase; D004259:DNA-Directed DNA Polymerase; D004121:Dimethyl Sulfoxide