Development and validation of a robust specific enzyme mediated assay for phenylalanine in serum.
The specificity of a phenylalanine dehydrogenase, particularly with respect to cross reactivity toward tyrosine, has been shown to be pH dependent, being minimal at high pH. The dehydrogenase step has been coupled to colorimetric detection of NADH using a tetrazolium salt. The assay shows no significant cross reactivity towards a range of amino acids or drugs and correlates well with an established HPLC technique.
Campbell
R S
RS
Department of Clinical Biochemistry, London Hospital Medical College, UK.
Brearley
G M
GM
Varsani
H
H
Morris
H C
HC
Milligan
T P
TP
Hall
S K
SK
Hammond
P M
PM
Price
C P
CP
D000594:Amino Acid Oxidoreductases / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D004796:Clinical Enzyme Tests / Q000379:methods*; D003124:Colorimetry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D009243:NAD / Q000378:metabolism; D010649:Phenylalanine / Q000097:blood*; D015203:Reproducibility of Results; D013778:Tetrazolium Salts / Q000737:chemistry; D014443:Tyrosine / Q000737:chemistry
D013778:Tetrazolium Salts; D009243:NAD; D014443:Tyrosine; D010649:Phenylalanine; D000594:Amino Acid Oxidoreductases; C022342:phenylalanine oxidase