Acrylamide polymerization kinetics in gel electrophoresis capillaries. A Raman microprobe study.
The formation of 3.5% T, 3.3% C cross-linked polyacrylamide is monitored in 75-microns-i.d. electrophoresis capillaries by Raman microprobe spectroscopy. The disappearance of the acrylamide 1292-cm-1 band is followed with 60-s time resolution for 30 min, and 2-4 min resolution for up to 10 h. Polymerization is 98% complete in 1.5 h and greater than 99% complete after 2 h. In the 900-1700-cm-1 region no bands attributable to cross-linking are observable. Reaction in the capillary follows second-order kinetics. The reaction is faster in the bulk system because heat dissipation is not sufficient to maintain a constant temperature.
Rapp
T L
TL
Department of Chemistry, University of Michigan, Ann Arbor 48109-1055.
Kowalchyk
W K
WK
Davis
K L
KL
Todd
E A
EA
Liu
K L
KL
Morris
M D
MD
D020106:Acrylamide; D000178:Acrylamides / Q000737:chemistry*; D000180:Acrylic Resins / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D007700:Kinetics; D008832:Microchemistry; D011108:Polymers / Q000737:chemistry*; D013059:Spectrum Analysis, Raman
D000178:Acrylamides; D000180:Acrylic Resins; D011108:Polymers; C016680:polyacrylamide gels; D020106:Acrylamide