Identification of gram-negative bacteria by isoelectric focusing of soluble cellular proteins.
Strains of Escherichia coli, Pseudomonas aeruginosa and Proteus mirabilis, isolated from clinical specimens were disrupted by repeated freezing in liquid nitrogen and thawing at room temperature. The samples were separated by isoelectric focusing using polyacrylamide gels. The resulting protein patterns showed clear differences between the three species and made identification possible.
Margull
A
A
Laborarztpraxis, Ludwigsburg.
Süssmuth
R
R
Schulz
P
P
D001426:Bacterial Proteins / Q000032:analysis*; D004926:Escherichia coli / Q000737:chemistry*; D007525:Isoelectric Focusing; D007526:Isoelectric Point; D011513:Proteus mirabilis / Q000737:chemistry*; D011550:Pseudomonas aeruginosa / Q000737:chemistry*; D012995:Solubility
D001426:Bacterial Proteins