High-performance liquid chromatography-electrochemical detection of 3-methylhistidine in human urine.
A reversed-phase high-performance liquid chromatographic method is described for the determination of 3-methylhistidine content in human urine using pre-column derivatization with phenylisothiocyanate, isocratic elution with 15 mM sodium acetate-acetonitrile (92:8, v/v) and electrochemical detection. The limit of quantitation was 0.1 pmol. The method has been applied in routine analyses of 3-methylhistidine in both clinical and research work.
Betto
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P
Istituto Superiore di Sanità, Rome, Italy.
Ricciarello
G
G
Pichini
S
S
Dello Strologo
L
L
Rizzoni
G
G
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004563:Electrochemistry; D006801:Humans; D007223:Infant; D017879:Isothiocyanates; D007676:Kidney Failure, Chronic / Q000652:urine; D008762:Methylhistidines / Q000652:urine*; D013861:Thiocyanates / Q000737:chemistry
D017879:Isothiocyanates; D008762:Methylhistidines; D013861:Thiocyanates; C005441:phenylisothiocyanate; C028118:3-methylhistidine