Enzyme-linked immunosorbent assay for rat and human luteinizing hormone.
A sensitive and specific enzyme-linked immunosorbent assay (ELISA) suitable for measuring rat and human luteinizing hormone (LH) is described. The LH-ELISA used anti-bLH beta subunit monoclonal antibody-coated plates, an antiserum against rLH or hLH and an antibody against rabbit IgG labelled with peroxidase. Using rLH-RP-3 or hLH WHO IRP 68/40 as standard diluted in assay buffer, the LH-ELISA had a sensitivity of 50 pg/ml and 0.78 mIU/ml, respectively. The LH-ELISA allowed accurate determination of LH in buffer, cell culture media, anterior pituitary extracts and sera and was highly specific for rat and human LH. The use of this ELISA offers improvements in convenience, economy, sensitivity and safety over comparable radioimmunoassay procedures.
Leiva
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Instituto Chileno de Medicina Reproductiva, P. Universidad Católica de Chile, Facultad Ciencias Biológicas, Santiago.
de la Lastra
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D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D002478:Cells, Cultured; D003470:Culture Media / Q000737:chemistry; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D004971:Estrus / Q000502:physiology; D005260:Female; D006801:Humans; D007106:Immune Sera; D007986:Luteinizing Hormone / Q000032:analysis* / Q000097:blood / Q000276:immunology; D008297:Male; D051379:Mice; D010052:Ovariectomy; D010903:Pituitary Gland, Anterior / Q000737:chemistry / Q000378:metabolism; D011786:Quality Control; D051381:Rats; D011994:Recombinant Proteins / Q000276:immunology; D012016:Reference Values
D000911:Antibodies, Monoclonal; D003470:Culture Media; D007106:Immune Sera; D011994:Recombinant Proteins; D007986:Luteinizing Hormone