Antioxidant and free radical scavenging activities of Gastrodia elata Bl. and Uncaria rhynchophylla (Miq.) Jacks.
Gastrodia elata Bl. (GE) and Uncaria rhynchophylla (Miq.) Jacks (UR) are two traditional Chinese medicinal herbal drugs, used for the treatment of convulsions and epilepsy. Their antioxidant effects in vivo and their free radical scavenging effects in vitro were investigated. Epileptogenic foci in the lateral brain of the rat were induced by the injection of ferric chloride into the lateral cortex. Both extracts significantly inhibited the increase in levels of lipid peroxide in the ipsilateral cortex, at all times observed. In addition, the two extracts also induced an early increase of activity of superoxide dismutase in the mitochondrial fraction of the ipsilateral cortex. In in vitro experiments, the two extracts exhibited significant dose-dependent scavenging effects on free radicals, using electron spin resonance spectroscopy. These results suggest that the proposed antiepileptic effects of GE and UR may be attributable to the antioxidant activity of the active components in these two medicinal herbs.
Liu
J
J
Department of Neuroscience, Okayama University Medical School, Japan.
Mori
A
A
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001921:Brain / Q000187:drug effects* / Q000201:enzymology; D002712:Chlorides; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004827:Epilepsy / Q000139:chemically induced / Q000517:prevention & control; D005290:Ferric Compounds / Q000633:toxicity; D016166:Free Radical Scavengers; D008054:Lipid Peroxides / Q000378:metabolism; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000378:metabolism
D000975:Antioxidants; D002712:Chlorides; D004365:Drugs, Chinese Herbal; D005290:Ferric Compounds; D016166:Free Radical Scavengers; D008054:Lipid Peroxides; D013482:Superoxide Dismutase; C024555:ferric chloride