[A method of isolation and properties of DNA-dependent DNA-polymerase epsilon from human placenta].
DNA polymerase epsilon was purified to near homogeneity from human placenta. The enzyme has one subunit (170 kDa, sedimentation coefficient 8.2S), intrinsic 3'-5'-exonuclease activity, it is independent on PCNA and high processivity on poly(dA)-oligo(dT) template-primer without PCNA. It was shown, that the enzyme incorporates 3'-amino-2',3'-dideoxythymidine 5'-triphosphate in DNA, after that synthesis is stopped. Simultaneously DNA polymerase alpha was purified.
Mozzherin
D Iu
DIu
Atrazhev
A M
AM
Kukhanova
M K
MK
D001483:Base Sequence; D002853:Chromatography, Liquid; D004247:DNA / Q000096:biosynthesis; D004257:DNA Polymerase II / Q000302:isolation & purification / Q000378:metabolism; D004258:DNA Polymerase III; D004259:DNA-Directed DNA Polymerase / Q000302:isolation & purification* / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D008969:Molecular Sequence Data; D010920:Placenta / Q000201:enzymology*; D011247:Pregnancy; D013379:Substrate Specificity
D004247:DNA; D004257:DNA Polymerase II; D004258:DNA Polymerase III; D004259:DNA-Directed DNA Polymerase
Metod vydeleniia i svoĭstva DNK-zavisimoĭ DNK-polimerazy epsilon iz plztsenty cheloveka.