title: Subcellular localization and properties of adenosine diphosphatase in human placenta.
abstract: It was found that mitochondria from human placenta exhibited an ADPase activity with the following characteristics. The enzyme responsible for this activity was associated with the inner mitochondrial membrane. It was not released by treatment of the submitochondrial particles with solutions of high ionic strength. Maximal ADP hydrolysis was reached at pH 8. Specific inhibitors for alkaline phosphatase (L-phenylalanine), myokinase (P1,P5-di(adenosine-5')pentaphosphate), or 5'-nucleotidase (concanavalin A) did not decrease ADP hydrolysis. ATP synthesis from ADP by myokinase was about 13 nmol/mg/min, whereas ADP hydrolysis reached values around 500 to 550 nmol/mg/min, indicating that a myokinase-H+ATPase combination could not account for the observed rates of ADP hydrolysis. The activity was stimulated by Mg2+, but high concentrations of this cation produced inhibition. High ADP concentrations did not inhibit ADPase activity. Kinetic measurements of the activity in the submitochondrial particles showed that the true substrate was ADP-Mg. The kinetic studies showed V(app) values of 476 and 270 nmol/mg/min, and Kmapp values of 416 and 8.7 microM.
authors: Departamento de Bioquimica, Facultad de Medicina, Universidad Nacional Autonoma de México, México, D.F.
authors: F
authors: F
authors: Martínez
authors: R
authors: R
authors: Moncada
authors: F J
authors: FJ
authors: Barcenas
authors: T
authors: T
authors: Espinosa-García
mesh_terms: D000227:Adenine Nucleotides / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000096:biosynthesis; D001081:Apyrase / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D005260:Female; D006801:Humans; D006868:Hydrolysis; D007700:Kinetics; D008928:Mitochondria / Q000201:enzymology / Q000648:ultrastructure; D010920:Placenta / Q000201:enzymology*; D011247:Pregnancy; D013347:Subcellular Fractions / Q000201:enzymology*
chemical_list: D000227:Adenine Nucleotides; D000255:Adenosine Triphosphate; D001081:Apyrase