Sensitive micromethod for column liquid chromatographic determination of fluoxetine and norfluoxetine in human plasma.
A rapid, selective and sensitive micromethod has been developed for the determination of fluoxetine (FLU) and its demethylated metabolite norfluoxetine (N-FLU) using a 250-microliters plasma sample and column liquid chromatography with ultraviolet detection at 226 nm. The limit of detection is 2.0 ng/ml for both FLU and N-FLU. Peak-height ratios are linear over a concentration range of 10-800 and 10-1000 ng/ml for FLU and N-FLU, respectively. Acceptable coefficients of variation are demonstrated for both within-run and day-to-day assays. Selected drugs were checked for interference. The method, which requires a very small volume of plasma, is sensitive enough for pharmacokinetic studies in animals, clinical pharmacology studies and drug monitoring in children or adult patients.
Thomare
P
P
Département de Pharmacologie Clinique, Hôpital Pitié-Salpêtrière, Paris, France.
Wang
K
K
Van Der Meersch-Mougeot
V
V
Diquet
B
B
D002853:Chromatography, Liquid / Q000379:methods*; D005473:Fluoxetine / Q000031:analogs & derivatives* / Q000097:blood*; D006801:Humans; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet
D005473:Fluoxetine; C036139:norfluoxetine