[Validity of the AgNOR count in cervical pathology].
Nucleolar organizer regions (NORs) were counted on ten cases each of normal ectocervix, CIN 1, 2 and 3 to verify the possibility of a differentiation between the various grades of CIN and between them and condylomata. Counts were performed on the full thickness of the tissue, layer by layer (stratified counts). A significant difference (p < 0.05) was found between the means of normal tissue toward condylomata and CIN 2 and 3 and between CIN 1 and CIN 2 and 3. There was no significance (p < 0.05) between normal tissue and CIN 1, between CIN 2 and 3 and between condylomata and CIN 2 and 3. The range of variations on the counts was associated with overlapping between the various cases. Our data showed also a progressive rise in mean NOR values from normal tissue to CIN 3. The stratified counts showed in all the groups a rise from basal to parabasal cells. Counts on parabasal and intermediate layers distinguished two groups of cases. In one there was either the same number of dots or a further rise from one layer to the next, while in the other a definite decrease was seen. The former pattern may be related to a potential for malignant evolution of the lesion. NORs should be counted in all cases of CIN and condylomata to treat more aggressively those lesions which present the pattern of a progressive rise of NORs from basal to intermediate cells.
Leopardi
O
O
Servizio di Anatomia Patologica dell'Ospedale Maggiore di Lodi.
Colecchia
M
M
Colavecchio
A
A
D002278:Carcinoma in Situ / Q000648:ultrastructure*; D002584:Cervix Uteri / Q000473:pathology / Q000648:ultrastructure*; D003218:Condylomata Acuminata / Q000648:ultrastructure*; D005260:Female; D006801:Humans; D006965:Hyperplasia; D008679:Metaplasia; D009697:Nucleolus Organizer Region / Q000648:ultrastructure*; D016622:Silver Staining; D002583:Uterine Cervical Neoplasms / Q000648:ultrastructure*; D002575:Uterine Cervicitis / Q000473:pathology*
Validità della conta delle AgNORs nella patologia cervicale.