Isolation and initial characterization of a new virus: Micro-Foci inducing virus or MFV.
Infectious and transforming activity was obtained from cultures of a large kidney tumour with very high N-myc amplification. The patient belonged to a cluster of neuroblastoma cases diagnosed in Southern Louisiana in 1986-1988. Infection with ultra-filtered supernatants from neuroblastoma cultures caused the appearance of small foci of rounded cells with neural features and the presence of virus was indicated by electron microscopy: the virus was called Micro-Foci inducing virus or MFV. Infected young adult rats did not develop overt disease, but 11/11 of the litters from infected animals developed a very dramatic and highly lethal syndrome which could resemble neuroblastoma symptoms. N-myc amplification disappeared in very early cultures of the tumour, but it was subsequently detected in MFV-infected cell cultures.
Rovigatti
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I.O.C.M.H., Bobigny.
D000818:Animals; D002675:Child, Preschool; D005260:Female; D005784:Gene Amplification; D016259:Genes, myc / Q000235:genetics; D006801:Humans; D007680:Kidney Neoplasms / Q000382:microbiology*; D009447:Neuroblastoma / Q000382:microbiology*; D009858:Oncogenic Viruses / Q000235:genetics / Q000302:isolation & purification*; D051381:Rats; D011916:Rats, Inbred F344