title: Expression of human CD4 in transgenic mice does not confer sensitivity to human immunodeficiency virus infection.
abstract: Transfection of the human CD4 molecule into mouse cells does not confer susceptibility to human immunodeficiency virus type 1 (HIV-1) infection. Expression of the human CD4 molecule in transgenic mice was seen to offer some new possibilities. However, transgenic mouse T cells expressing either the human CD4 receptor, or a hybrid human/mouse CD4 receptor alone or in conjunction with human major histocompatibility complex class I molecules, were refractory to in vitro HIV-1 infection. In addition, no infection was observed after in vivo HIV inoculation to mice of these various transgenic lines. Injection of recombinant gp160 viral protein to the transgenic mice did not alter their T and B cell populations. The existence of a dominant block in mouse cells that prevents HIV entry is discussed.
authors: Unité 257, Institut National de la Santé et de la Recherche Médicale, Institut Cochin de Génétique Moléculaire, Paris, France.
authors: P
authors: P
authors: Lorès
authors: V
authors: V
authors: Boucher
authors: C
authors: C
authors: Mackay
authors: M
authors: M
authors: Pla
authors: H
authors: H
authors: Von Boehmer
authors: J
authors: J
authors: Jami
authors: F
authors: F
authors: Barré-Sinoussi
authors: J C
authors: JC
authors: Weill
mesh_terms: D000818:Animals; D001483:Base Sequence; D015704:CD4 Antigens / Q000235:genetics*; D004279:DNA, Viral / Q000235:genetics; D004195:Disease Models, Animal; D015870:Gene Expression; D015686:Gene Products, env / Q000276:immunology; D019302:HIV Envelope Protein gp160; D015658:HIV Infections / Q000209:etiology* / Q000235:genetics / Q000276:immunology; D015497:HIV-1 / Q000235:genetics / Q000276:immunology; D006801:Humans; D051379:Mice; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D011498:Protein Precursors / Q000276:immunology
chemical_list: D015704:CD4 Antigens; D004279:DNA, Viral; D015686:Gene Products, env; D019302:HIV Envelope Protein gp160; D011498:Protein Precursors