An mRNA that specifically accumulates in maize roots delineates a novel subset of developing cortical cells.
A near full-length cDNA clone (pZRP3) corresponding to an mRNA that accumulates specifically in roots of maize was isolated. The ZRP3 mRNA is ca. 600 nucleotides in length. The amino acid sequence of the predicted polypeptide is rich in leucine (16%), proline (11%), and cysteine (8.5%). The zrp3 gene appears to be expressed exclusively in roots, whereas other ZRP3-related genes are expressed in additional organs of the maize plant. In situ hybridization shows that ZRP3 mRNA accumulation is largely confined to the cells of the cortical ground meristem. Furthermore, accumulation of this mRNA occurs within a distinct subset of cortical cells, the inner three to four cell layers.
John
I
I
Department of Botany, Iowa State University, Ames 50011-1020.
Wang
H
H
Held
B M
BM
Wurtele
E S
ES
Colbert
J T
JT
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D003545:Cysteine / Q000032:analysis; D015723:Gene Library; D005796:Genes; D017403:In Situ Hybridization; D007930:Leucine / Q000032:analysis; D045342:Light-Harvesting Protein Complexes; D008969:Molecular Sequence Data; D045322:Photosynthetic Reaction Center Complex Proteins / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics*; D011392:Proline / Q000032:analysis; D011487:Protein Conformation; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D003313:Zea mays / Q000235:genetics* / Q000378:metabolism*
D045342:Light-Harvesting Protein Complexes; D045322:Photosynthetic Reaction Center Complex Proteins; D010940:Plant Proteins; D012333:RNA, Messenger; C078708:ZRP3 protein, Zea mays; D011392:Proline; D007930:Leucine; D003545:Cysteine