Interdependence of respiratory NO reduction and nitrite reduction revealed by mutagenesis of nirQ, a novel gene in the denitrification gene cluster of Pseudomonas stutzeri.
An open reading frame, designated nirQ, was identified upstream of nirS, the structural gene for the respiratory nitrite reductase of Pseudomonas stutzeri ZoBell. Its derived gene product (275 amino acids, M(r) = 30,554) shows similarity to the NtrC protein family of transcriptional activators. Deletion-replacement mutagenesis of the nirQ gene resulted in the simultaneous loss of nitrite reduction and NO reduction in vivo. However, both reductases were still synthesized, with only nitrite reductase being active in vitro. NO reductase was overproduced by a factor of about 2. Our results indicate that the systems for nitrite reduction and NO reduction are functionally coupled.
Jüngst
A
A
Lehrstuhl für Mikrobiologie, Universität Karlsruhe, Germany.
Zumft
W G
WG
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D005810:Multigene Family; D016296:Mutagenesis; D009569:Nitric Oxide / Q000378:metabolism*; D009573:Nitrites / Q000378:metabolism*; D010084:Oxidation-Reduction; D011549:Pseudomonas / Q000235:genetics* / Q000378:metabolism; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D015534:Trans-Activators
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D009573:Nitrites; D015534:Trans-Activators; C078738:nirQ protein, Pseudomonas; D009569:Nitric Oxide