Improved high-performance liquid chromatographic determination of ciprofloxacin and its metabolites in human specimens.
A simple approach to the quantitation of ciprofloxacin and its three metabolites, M1 (desethylene-ciprofloxacin), M2 (sulfo-ciprofloxacin) and M3 (oxo-ciprofloxacin), in human serum, urine, saliva and sputum is described. This assay allows the parent drug and its metabolites to elute and be resolved in a single chromatogram at 280 nm using a linear gradient. The procedure involved liquid-liquid extraction. Separation was achieved on a C18 reversed-phase column. The limit of detection of ciprofloxacin is 0.05 microgram/ml and that of its three metabolites is 0.25 microgram/ml. This method is sufficiently sensitive for pharmacokinetic studies.
Mack
G
G
Department of Paediatrics, Westmead Hospital, N.S.W., Australia.
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002939:Ciprofloxacin / Q000032:analysis* / Q000097:blood / Q000652:urine; D006801:Humans; D012463:Saliva / Q000737:chemistry*; D013183:Sputum / Q000737:chemistry*
D002939:Ciprofloxacin