Applicability of an immunoblot technique combined with a semiautomated electrophoresis system for detection of staphylococcal enterotoxins in food extracts.
We studied the usefulness of an immunoblot technique for the detection of staphylococcal enterotoxins (SEs) in strains and food extracts. Food samples (milk, yogurt, hot dog sausage, cheese, and mayonnaise) were artificially contaminated with SEA through SEE. Protein A did not interfere with the results; it appeared on electrophoresis gels as bands with molecular weights higher than those of the SEs. Other food proteins were not revealed by the technique. The immunoblot technique proved to be fast, specific, and sensitive for the detection of SEs in foods.
Orden
J A
JA
Departamento de Patologia Animal I, Facultad de Veterinaria, Universidad Complutense, Madrid, Spain.
Goyache
J
J
Hernández
J
J
Doménech
A
A
Suárez
G
G
Gómez-Lucía
E
E
D004591:Electrophoresis, Polyacrylamide Gel; D004768:Enterotoxins / Q000302:isolation & purification*; D005069:Evaluation Studies as Topic; D005506:Food Contamination; D005516:Food Microbiology; D006801:Humans; D015151:Immunoblotting / Q000379:methods* / Q000706:statistics & numerical data; D012680:Sensitivity and Specificity; D013202:Staphylococcal Food Poisoning / Q000517:prevention & control; D013211:Staphylococcus aureus / Q000302:isolation & purification*
D004768:Enterotoxins