A CD4-derived peptide carrier blocks acute HIV-1 infection in vitro and binds to gp120 in the presence of Walter-Reed stage 1-6 HIV+ sera.
A peptide containing amino acid residues 41-84 of the CD4 molecule was synthesized and coupled through a thioether bond to human serum albumin. This conjugate bound to gp120 with an affinity that was half that of CD4 and blocked the HIV infection in vitro with an efficacy tenfold lower than that of CD4. More importantly, the CD4 peptide-human serum albumin conjugate could bind to gp120 in the presence of HIV+ sera from 18 Walter Reed stage 1-6 patients.
Ghetie
V
V
Department of Microbiology, University of Texas Southwestern Medical Center, Dallas 75235-9048.
Wheeler
T
T
Scott
D
D
Uhr
J W
JW
Vitetta
E S
ES
D000163:Acquired Immunodeficiency Syndrome / Q000276:immunology*; D000937:Antigen-Antibody Reactions; D001667:Binding, Competitive; D015704:CD4 Antigens / Q000276:immunology* / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D015483:HIV Antibodies / Q000276:immunology*; D015699:HIV Envelope Protein gp120 / Q000378:metabolism*; D015497:HIV-1 / Q000187:drug effects / Q000254:growth & development / Q000276:immunology*; D006801:Humans; D010446:Peptide Fragments / Q000276:immunology* / Q000494:pharmacology; D012709:Serum Albumin / Q000276:immunology
D015704:CD4 Antigens; D015483:HIV Antibodies; D015699:HIV Envelope Protein gp120; D010446:Peptide Fragments; D012709:Serum Albumin