Calf chymosin as a catalyst of peptide synthesis.
Calf chymosin was shown to catalyse peptide synthesis optimally over the range pH 4-5, giving satisfactory yields of methyl esters or p-nitroanilides of benzyloxycarbonyl tetra- to hexa-peptides, provided that hydrophobic amino-acid residues form the new peptide bonds. The effectiveness of the enzyme depends also on the nature of adjacent amino-acid residues. As an aspartate-proteinase with a characteristic specificity pattern chymosin would be useful for the synthesis of middle-length peptides.
Abdel Malak
C A
CA
Department of Chemistry, Moscow State University, Russia.
D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D002625:Chemistry, Organic / Q000379:methods; D012085:Chymosin / Q000737:chemistry*; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis*
D010455:Peptides; D012085:Chymosin