The use of post-embedding immunoelectron microscopy in the diagnosis of glomerular diseases. Comparison of immunoelectron microscopic and immunofluorescence studies.
Fifty renal biopsies were studied by immunoelectron microscopy after embedding in a partly hydrophilic polyacrylic resin (LR White). Immunofluorescence studies were carried out on frozen sections of parallel tissue samples. Polyacrylic embedding gave good preservation of the renal ultrastructure and precise localization of immunoglobulin and C3c antibodies within glomerular electron-dense deposits. Non-specific staining of plasma proteins within vascular lumina could easily be detected. There was good correlation between immunoelectron and immunofluorescence microscopy. Immunoelectron microscopy is a very sensitive method, which can detect small amounts of antigen. More cases were, however, positive by immunofluorescence than by immunoelectron microscopy. This discrepancy may be explained by difference in sample size, and by difference in resolution of morphological details (electron microscopy versus fluorescence microscopy).
Skjørten
F J
FJ
Department of Pathology, Ullevaal Hospital, Oslo, Norway.
Brorson
S H
SH
Roald
B
B
Strøm
E H
EH
Lund
B
B
D000180:Acrylic Resins; D001706:Biopsy; D005455:Fluorescent Antibody Technique; D005921:Glomerulonephritis / Q000175:diagnosis; D006801:Humans; D006977:Hypertension, Renal / Q000175:diagnosis; D007136:Immunoglobulins / Q000378:metabolism; D007674:Kidney Diseases / Q000175:diagnosis*; D007678:Kidney Glomerulus / Q000276:immunology / Q000648:ultrastructure; D016253:Microscopy, Immunoelectron / Q000379:methods*; D016610:Tissue Embedding
D000180:Acrylic Resins; D007136:Immunoglobulins