Immunoaffinity-chromatography purification of salbutamol in liver and HPLC-fluorometric detection at trace residue level.
A method combining immunoaffinity-chromatography (IAC) and high-performance liquid chromatography (HPLC) for the analysis of Salbutamol in liver with a low quantification limit of 1 micrograms/kg has been developed. Salbutamol was extracted with 0.01 mol/L HCl and purified by IAC. The samples were analysed on a liquid chromatograph fitted with a C18 mu-Bondapak column. A fluorometer was used for the detection of salbutamol. Recoveries of 67-80% could be obtained.
Degroodt
J M
JM
Ministère de la Santé Publique et de l'Environnement, Bruxelles, Belgium.
Wyhowski de Bukanski
B
B
Srebrnik
S
S
D000420:Albuterol / Q000032:analysis / Q000302:isolation & purification*; D000818:Animals; D002417:Cattle; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D004350:Drug Residues / Q000032:analysis / Q000302:isolation & purification*; D008099:Liver / Q000737:chemistry*; D008460:Meat / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence
D000420:Albuterol