A degU-containing SP beta prophage complements superactivator mutations affecting the Bacillus subtilis degSU operon.
The Bacillus subtilis degSU two-gene operon encodes a two-component regulatory system which positively controls expression of a variety of genes encoding degradative enzymes. Point superactivator mutations in either degS or degU increase the production of these enzymes. A specialized transducing SP beta phage which partially complements several of these mutations was isolated from a phage library of the B. subtilis chromosome. This phage was shown to contain the degU (wild-type) gene. This unexpected co-dominance is discussed.
Podvin
L
L
Laboratoire de Génétique des Microorganismes, Institut National Agronomique Paris-Grignon, CNRS, France.
Steinmetz
M
M
D017103:Bacillus Phages / Q000235:genetics*; D001412:Bacillus subtilis / Q000235:genetics*; D066298:In Vitro Techniques; D008242:Lysogeny; D016254:Mutagenesis, Insertional; D009876:Operon / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D013489:Suppression, Genetic; D014161:Transduction, Genetic / Q000235:genetics*