The role of the kininogens as cysteine proteinase inhibitors in local and systemic inflammation.
The contribution of the kininogens and cystatin C to the functional inhibitory capacity for cysteine proteinases of blood plasma and inflammatory secretions was estimated from ex vivo experiments. 98.5% of the inhibitory capacity of blood plasma for cathepsin L (4-5 microM) is provided by the kininogens ensuring a complete control of this enzyme even at a lowered kininogen concentration. Control of cathepsin B activity by the kininogens is incomplete and depends critically on the active concentration of cystatin C (70 nM in normal plasma), which is reduced in blood plasma of polytraumatized and septic patients and very low in epithelial lining fluid of the shock lung.
Assfalg-Machleidt
I
I
Institut für Physiologische Chemie, Physikalische Biochemie und Zellbiologie, Universität München, Germany.
Billing
A
A
Fröhlich
D
D
Nast-Kolb
D
D
Joka
T
T
Jochum
M
M
Machleidt
W
W
D002401:Cathepsin B / Q000037:antagonists & inhibitors; D056668:Cathepsin L; D002403:Cathepsins / Q000037:antagonists & inhibitors; D015891:Cystatins / Q000502:physiology; D003546:Cysteine Endopeptidases; D010450:Endopeptidases; D006801:Humans; D066298:In Vitro Techniques; D007249:Inflammation / Q000503:physiopathology*; D007700:Kinetics; D007704:Kininogens / Q000097:blood / Q000502:physiology*; D010206:Papain / Q000037:antagonists & inhibitors
D015891:Cystatins; D007704:Kininogens; D002403:Cathepsins; D010450:Endopeptidases; D003546:Cysteine Endopeptidases; D002401:Cathepsin B; C534281:CTSL protein, human; D056668:Cathepsin L; D010206:Papain