A preparation and purification of [1-14C]acetylcarnitine.
[1-14C]Acetylcarnitine was prepared from [1-14C]acetate and L-carnitine using acetyl-CoA synthetase and carnitine acetyltransferase. The product was purified by ion-exchange and thin-layer chromatography. Conversion of [1-14C]acetate to [1-14C]acetylcarnitine was better than 90% and overall recovery of the pure product was greater that 80%.
Roughan
G
G
Horticulture Research Institute, Auckland, New Zealand.
Browse
J
J
D000085:Acetates / Q000737:chemistry; D000108:Acetylcarnitine / Q000138:chemical synthesis* / Q000302:isolation & purification; D002250:Carbon Radioisotopes; D002331:Carnitine / Q000737:chemistry; D002332:Carnitine O-Acetyltransferase / Q000378:metabolism; D002852:Chromatography, Ion Exchange / Q000379:methods; D002855:Chromatography, Thin Layer / Q000379:methods; D003066:Coenzyme A Ligases / Q000378:metabolism; D007553:Isotope Labeling / Q000379:methods; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; D013997:Time Factors; D000098859:Long-Chain-Fatty-Acid-CoA Ligase
D000085:Acetates; D002250:Carbon Radioisotopes; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; D000108:Acetylcarnitine; D002332:Carnitine O-Acetyltransferase; D003066:Coenzyme A Ligases; C090799:FAA2 protein, S cerevisiae; D000098859:Long-Chain-Fatty-Acid-CoA Ligase; D002331:Carnitine