Effects of endothelin and calcium channel blockers on membrane voltage and intracellular calcium in cultured bovine trabecular meshwork cells.
The membrane voltage was measured in cultured bovine trabecular meshwork cells. External barium (Ba2+; 10 mM) evoked repetitive overshooting action potentials which were reversibly inhibited by 10(-6) M nifedipine but insensitive to tetrodotoxin (10(-5) M). In contrast, no action potentials could be induced in bovine corneal endothelial cells. The vasoactive peptide endothelin (10(-9)-10(-6) M) induced dose-dependent depolarizations. The intracellular calcium concentration measured by the fura-2 method increased after endothelin application. An initial peak was followed by a sustained plateau. The effect of endothelin on the membrane potential and intracellular calcium value is typical for smooth muscle cells showing a contractile response to the peptide. Recent studies have shown the contractility of bovine trabecular meshwork. We conclude that calcium-channel antagonists and endothelin may alter the aqueous humour outflow through the trabecular meshwork.
Lepple-Wienhues
A
A
Institut für klinische Physiologie, Klinikum Steglitz der Freien Universität Berlin, Federal Republic of Germany.
Stahl
F
F
Wunderling
D
D
Wiederholt
M
M
D000818:Animals; D001464:Barium / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D002121:Calcium Channel Blockers / Q000494:pharmacology*; D002417:Cattle; D002478:Cells, Cultured; D016232:Endothelins / Q000494:pharmacology*; D016257:Fura-2 / Q000378:metabolism; D008564:Membrane Potentials / Q000187:drug effects*; D008839:Microelectrodes; D013779:Tetrodotoxin / Q000494:pharmacology; D014129:Trabecular Meshwork / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology
D002121:Calcium Channel Blockers; D016232:Endothelins; D001464:Barium; D013779:Tetrodotoxin; D002118:Calcium; D016257:Fura-2