[The effect of inhibitors of glucose utilization on the chemiluminescence and fluorescence of murine peritoneal macrophages].
Using the inhibitors of glucose utilization and mitochondrial oxidation we found that the blue autofluorescence (AF) of murine peritoneal macrophages (MP) originates predominantly from the cytosolic NAD(P)H. AF intensity correlates with the changes in glycolytic enzymes activity. The luminol-dependent chemiluminescence (CL) intensity which characterizes the amount of O2- generating by MP upon the addition of formyl-methionyl-leucyl-phenylalanine (FMLP) is inhibited in the case of maximum activity of glycolytic enzymes. Incubation of MP with non-convertible analog of glucose, such as 2-deoxyglucose increases the CL intensity under the influence of FMLP. A conclusion is drawn that amount of O2- generating by MP depends on the intracellular concentration of sugars.
Kliubin
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D000818:Animals; D003864:Depression, Chemical; D005453:Fluorescence; D005947:Glucose / Q000037:antagonists & inhibitors* / Q000378:metabolism; D006019:Glycolysis / Q000187:drug effects; D008163:Luminescent Measurements; D008262:Macrophage Activation / Q000187:drug effects / Q000502:physiology; D008264:Macrophages / Q000187:drug effects* / Q000201:enzymology; D051379:Mice; D008810:Mice, Inbred C57BL; D008856:Microscopy, Fluorescence; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D010529:Peritoneal Cavity / Q000166:cytology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D015054:Zymosan / Q000494:pharmacology
D009240:N-Formylmethionine Leucyl-Phenylalanine; D015054:Zymosan; D005947:Glucose; D013755:Tetradecanoylphorbol Acetate
Vliianie ingibitorov utilizatsii gliukozii na khemiliuminestsentsiiu i fluorestsentsiiu myshinykh peritoneal'nykh makrofagov.