title: A simple method for the isolation of zymosterol from a sterol mutant of Saccharomyces cerevisiae.
abstract: A simple method is described for the direct isolation of zymosterol (5 alpha-cholesta-8,24-dien-3 beta-ol) of high purity from a sterol mutant of Saccharomyces cerevisiae. This yeast strain, which is a double mutant of the ERG6 (sterol transmethylase) and ERG2 (C-8 sterol isomerase) genes, accumulates zymosterol as its major sterol component.
authors: Department of Chemistry, Auburn University, AL 36849.
authors: R W
authors: RW
authors: Heiderpriem
authors: P D
authors: PD
authors: Livant
authors: E J
authors: EJ
authors: Parish
authors: R J
authors: RJ
authors: Barbuch
authors: M G
authors: MG
authors: Broaddus
authors: M
authors: M
authors: Bard
mesh_terms: D002784:Cholesterol; D012441:Saccharomyces cerevisiae / Q000737:chemistry*; D013261:Sterols / Q000302:isolation & purification*
chemical_list: D013261:Sterols; D002784:Cholesterol; C015582:zymosterol