Oncogenic transformation by Jun: role of transactivation and homodimerization.
Jun/JunD and Jun/GCN4 chimeras transform chicken embryo fibroblasts and activate the collagenase promoter in these same cells. Individual constructs differ widely in the two activities, and there is no correlation between transformation and transactivation. These results suggest that oncogenic transformation by Jun is not caused merely by an upregulation of AP-1 activity. Jun constructs with a modified dimerization domain allowing only homodimerization are active in transformation and transactivation in chicken embryo fibroblast cultures. Homodimers of Jun therefore transform and transactivate.
Hartl
M
M
Department of Microbiology, University of Southern California School of Medicine, Los Angeles 90033.
Vogt
P K
PK
D000818:Animals; D001704:Biopolymers; D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D002642:Chick Embryo; D005347:Fibroblasts / Q000502:physiology; D005822:Genetic Vectors / Q000235:genetics; D009154:Mutation / Q000235:genetics; D017434:Protein Structure, Tertiary; D016755:Proto-Oncogene Proteins c-jun / Q000502:physiology*; D011994:Recombinant Proteins; D014158:Transcription, Genetic / Q000235:genetics; D015533:Transcriptional Activation / Q000235:genetics*
D001704:Biopolymers; D016755:Proto-Oncogene Proteins c-jun; D011994:Recombinant Proteins