The effect of calcium on DNA synthesis in pea (Pisus sativus L.) roots after treatment with heavy metals.
Cortex cells of the meristematic (1 mm) and differentiated (7 mm) zones of Pisus sativus L. roots after 144 h culture in distilled water (control), Ca2+ (10(-3) M) and/or Cd2+, Cr3+, Pb2+ solutions (10(-4) M, each) were subject of the present studies. Reductions in the number of nuclei incorporating 3H thymidine was observed in meristem in the presence of Cd2+ and in differentiated zone in Pb2+ treated roots. Intensity of DNA synthesis diminished after Pb2+ treatment but mostly in Cd2+ treated roots, mainly in meristem. Addition of calcium to the metal solutions caused an increase in the number of nuclei witch uptook the radioactive material in both studied root zones. Positive effect of calcium alone was visible also in the differentiated zone. Presence of calcium in the metal solutions caused a marked increase in 3H thymidine incorporation into nuclear DNA in meristem, although neutralizing calcium effect in this root zone was visible only in roots treated with Cr2+ and Pb2+. In the differentiated zone of roots growing in Cd2+ solution, calcium addition stimulated DNA synthesis but the intensity of this process was lower than in control (water).
Gabara
B
B
Department of Cytology and Cytochemistry, University of Lódź, Poland.
Wojtyła-Kuchta
B
B
Tarczyńska
M
M
D002118:Calcium / Q000494:pharmacology*; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism; D002857:Chromium / Q000493:pharmacokinetics / Q000633:toxicity; D004247:DNA / Q000096:biosynthesis*; D004357:Drug Synergism; D007887:Fabaceae / Q000187:drug effects / Q000378:metabolism*; D007854:Lead / Q000493:pharmacokinetics / Q000633:toxicity; D008670:Metals / Q000493:pharmacokinetics / Q000633:toxicity*; D010946:Plants, Medicinal; D013936:Thymidine / Q000378:metabolism; D014316:Tritium
D008670:Metals; D002857:Chromium; D014316:Tritium; D007854:Lead; D004247:DNA; D002118:Calcium; D013936:Thymidine