A technique for studies of the contractile apparatus in single human muscle fibre segments obtained by percutaneous biopsy.
Human muscle samples were obtained with the percutaneous biopsy technique. The samples were membrane-hyperpermeabilized (skinned) using a chemical or freeze-drying technique. Short single fibre segments were dissected from the sample, transferred to an experimental chamber, connected to a force transducer and manipulator, and exposed to temperature-controlled solutions. The force generating-capacity, the sensitivity of the contractile apparatus to calcium and the caffeine threshold for calcium release from the sarcoplasmic reticulum could be studied in the short muscle fibre segments obtained from man with the percutaneous muscle biopsy technique. The average length of the fibre segments between the connectors was 0.44 +/- 0.21 mm. Thus, detailed studies of the contractile machinery can be made on human skinned muscle fibres with only minimal discomfort to the patient or subject during biopsy, which should be useful in studies of neuromuscular disease, muscle plasticity or in applied physiology.
Larsson
L
L
Department of Clinical Neurophysiology, Karolinska Hospital, Stockholm, Sweden.
Salviati
G
G
D001696:Biomechanical Phenomena; D001707:Biopsy, Needle; D002110:Caffeine / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D005612:Freeze Drying; D006801:Humans; D066298:In Vitro Techniques; D009119:Muscle Contraction / Q000187:drug effects / Q000502:physiology*; D009132:Muscles / Q000033:anatomy & histology / Q000187:drug effects / Q000502:physiology*; D010827:Physiology / Q000379:methods
D002110:Caffeine; D002118:Calcium