Studies on thermophile products. V. Immunosuppressive profile in vitro of Bacillus stearothermophilus component, Fr.5-B.
The immunosuppressive profile of Bacillus stearothermophilus UK563 component, Fr.5-B, is presented in in vitro studies. Fr.5-B (0.1-1000 ng/ml), provided it was added at the initiation of mixed leukocyte reaction (MLR), inhibited dose-dependently the incorporation of tritiated thymidine ([3H]TdR) into mouse spleen cells and human peripheral blood lymphocytes. Even the addition of Fr.5-B 48 h after the onset of culture suppressed mouse MLR, unlike cyclosporin A (CYA). Fr.5-B significantly inhibited cytotoxic T lymphocyte generation determined by [3H]TdR-release micro-cytotoxicity assay by using mouse mastocytoma P815 as targets. Moreover, this component decreased dose-dependently the expression of class II major histocompatibility molecules (Ia) on mouse peritoneal macrophages induced by concanavalin A supernatant. The present results revealed the unique immunosuppressive property of Fr.5-B which was different from that of CYA.
Kohama
Y
Y
Faculty of Pharmaceutical Sciences, Osaka University, Japan.
Tanaka
K
K
Murayama
N
N
Itoh
M
M
Suda
T
T
Takae
S
S
Mimura
T
T
D000818:Animals; D002478:Cells, Cultured; D016572:Cyclosporine / Q000494:pharmacology; D003602:Cytotoxicity, Immunologic / Q000187:drug effects; D005260:Female; D001411:Geobacillus stearothermophilus / Q000737:chemistry*; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D006801:Humans; D007108:Immune Tolerance; D007166:Immunosuppressive Agents / Q000276:immunology* / Q000494:pharmacology*; D007959:Lymphocyte Culture Test, Mixed; D008264:Macrophages / Q000187:drug effects / Q000276:immunology; D051379:Mice; D009930:Organic Chemicals; D013602:T-Lymphocytes, Cytotoxic / Q000187:drug effects / Q000276:immunology; D014407:Tumor Cells, Cultured
C079029:Fr.5-B, Bacillus stearothermophilus UK563 component; D000949:Histocompatibility Antigens Class II; D007166:Immunosuppressive Agents; D009930:Organic Chemicals; D016572:Cyclosporine