title: A novel vesicle-associated protein (VAP-1) in sea urchin eggs containing multiple RNA-binding consensus sequences.
abstract: We have identified a novel high molecular weight, vesicle-associated protein (VAP-1) in the eggs of the sea urchin Strongylocentrotus purpuratus. Biochemical fractionation and immunofluorescence analysis of unfertilized eggs indicate that VAP-1 is a peripheral membrane protein associated with microsomal membrane fractions. Sequence analysis of partial VAP-1 cDNA clones reveals that the protein contains at least four RNA-binding consensus sequences. The RNA-binding sequences are separated by several glycine rich domains and this organization, RNA-binding domains separated by glycine rich sequences, is common to several RNA-binding proteins including the heterogeneous ribonuclear protein A1 and nucleolin. The characteristics of VAP-1 suggest that the protein may function as a multidomain RNA-binding protein. The possibility that VAP-1 may play a role in nuclear RNA processing is also discussed.
authors: Department of Cell Biology and Anatomy, University of Miami School of Medicine, FL 33101.
authors: N R
authors: NR
authors: Barton
authors: E M
authors: EM
authors: Bonder
authors: D J
authors: DJ
authors: Fishkind
authors: R H
authors: RH
authors: Warren
authors: M M
authors: MM
authors: Pratt
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D016384:Consensus Sequence; D004527:Egg Proteins / Q000737:chemistry / Q000235:genetics*; D005998:Glycine; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics*; D008861:Microsomes / Q000378:metabolism; D008969:Molecular Sequence Data; D010063:Ovum / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000737:chemistry / Q000235:genetics*; D012617:Sea Urchins / Q000235:genetics*; D016415:Sequence Alignment
chemical_list: D004527:Egg Proteins; D008565:Membrane Proteins; D016601:RNA-Binding Proteins; C079097:vesicle-associated protein, sea urchin; D005998:Glycine