Suppression of radiation-induced neoplastic transformation by overexpression of mitochondrial superoxide dismutase.
Manganese superoxide dismutase (MnSOD) scavenges toxic superoxide radicals produced in the mitochondria. Transfection of the human MnSOD gene into mouse C3H 10T1/2 cells resulted in production of active MnSOD, which was properly transported into mitochondria. Overexpression of MnSOD protected cells from radiation-, but not chemically-induced neoplastic transformation. This finding demonstrates that oxidative stress that occurs in the mitochondria plays an important role in the development of neoplastic transformation.
St Clair
D K
DK
Graduate Center for Toxicology, University of Kentucky, Lexington 40506-0054.
Wan
X S
XS
Oberley
T D
TD
Muse
K E
KE
St Clair
W H
WH
D000818:Animals; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000378:metabolism*; D004307:Dose-Response Relationship, Radiation; D016166:Free Radical Scavengers; D005720:Gamma Rays; D006801:Humans; D051379:Mice; D008809:Mice, Inbred C3H; D016253:Microscopy, Immunoelectron; D008928:Mitochondria / Q000201:enzymology* / Q000648:ultrastructure; D009368:Neoplasm Transplantation; D009381:Neoplasms, Radiation-Induced / Q000473:pathology / Q000517:prevention & control*; D012513:Sarcoma, Experimental / Q000473:pathology / Q000517:prevention & control*; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism*; D014162:Transfection
D016166:Free Radical Scavengers; D013482:Superoxide Dismutase