[Expansion of peripheral blood lymphocytes by culture with immobilized anti-CD3 antibody and IL-2].
Peripheral blood lymphocytes separated from about 20ml of blood were cultured with immobilized anti-CD3 antibody and IL-2. Total T lymphocytes in the culture expanded about 2,000 times by 2 weeks culture. Expanded T lymphocytes were infused to cancer patients, and safety and feasibility was confirmed. This method will be applicable for prevention of cancer recurrence.
Sekine
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National Cancer Center Research Institute, Tokyo, Japan.
D000328:Adult; D000368:Aged; D017252:CD3 Complex / Q000627:therapeutic use*; D002292:Carcinoma, Renal Cell / Q000628:therapy; D002455:Cell Division; D002469:Cell Separation / Q000379:methods; D005260:Female; D006801:Humans; D016219:Immunotherapy, Adoptive; D007376:Interleukin-2 / Q000627:therapeutic use*; D007680:Kidney Neoplasms / Q000628:therapy; D007958:Leukocyte Count; D008297:Male; D008875:Middle Aged; D013601:T-Lymphocytes / Q000166:cytology* / Q000637:transplantation; D014407:Tumor Cells, Cultured
D017252:CD3 Complex; D007376:Interleukin-2