Homology of an acrosome-reacted sperm-specific antigen to CD46.
MH61 is an acrosome-reacted sperm-specific monoclonal antibody. The antibody-coated beads effectively analyze the acrosomal status of human sperm. However, the nature of the antigen was not known. The antigen was purified by immunoprecipitation and SDS-PAGE followed by blotting on PVDF paper. The N-terminal sequence of the antigen was analyzed by an automated protein sequencer. An exactly matching sequence was found in CD46, that is also known as a membrane cofactor protein.
Okabe
M
M
Faculty of Pharmaceutical Sciences, Osaka University, Japan.
Ying
X
X
Nagira
M
M
Ikawa
M
M
Kohama
Y
Y
Mimura
T
T
Tanaka
K
K
D000177:Acrosome / Q000502:physiology; D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000737:chemistry*; D000954:Antigens, Surface / Q000737:chemistry* / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008297:Male; D051916:Membrane Cofactor Protein; D008562:Membrane Glycoproteins / Q000737:chemistry*; D008969:Molecular Sequence Data; D011233:Precipitin Tests; D012661:Semen; D017386:Sequence Homology, Amino Acid; D013094:Spermatozoa / Q000276:immunology*
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000954:Antigens, Surface; C497857:CD46 protein, human; D051916:Membrane Cofactor Protein; D008562:Membrane Glycoproteins