title: Aeroallergen analyses and their clinical relevance. II. Sampling by high-volume airsampler with immunochemical quantification versus Burkard pollen trap sampling with morphologic quantification.
abstract: A comparison was made between the amount of airborne pollen collected by Burkard airsampler and the allergenic activity of particles trapped on glass fibre filters in an Accu-Vol high-volume airsampler. The comparison was made throughout the pollen seasons 1986 to 1989. Both airsamplers were operated 24 h a day. They were placed less than 5 m apart, and estimation of the pollen amount was made on a day-to-day basis during the pollen seasons, and on a weekly basis outside the seasons. The occurrence of the 3 clinically most important allergenic types of pollen, birch, grass, and mugwort, was analysed, and close correlations between the 2 sampling techniques were found (rs 0.5-0.8, p < 0.001). The detected range of counted pollens/m3 was: birch 0-1075, grass 0-156, and mugwort 0-44. By immunochemical analysis we found the corresponding amounts to be 0-80, 0-8, and 0-1 SQ-U/m3, respectively. Pollen counts and immunochemical estimation were compared with the symptom score recordings of allergic persons for birch season 1989 and for grass seasons 1986, 1988, and 1989. A close correlation was found for both sampling techniques for the grass seasons in 1986 and 1989 (rs 0.51-0.61, p < 0.001-0.0001), but a less significant correlation was found for the 1988 grass season, and for birch in 1989 (rs 0.24-0.34, p < 0.001-0.05).
authors: Laboratory of Medical Allergology, National University Hospital, Copenhagen, Denmark.
authors: C R
authors: CR
authors: Johnsen
authors: E R
authors: ER
authors: Weeke
authors: J
authors: J
authors: Nielsen
authors: J
authors: J
authors: Jensen
authors: H
authors: H
authors: Mosbech
authors: L
authors: L
authors: Frølund
authors: F
authors: F
authors: Madsen
authors: L K
authors: LK
authors: Poulsen
mesh_terms: D000393:Air Pollutants / Q000032:analysis*; D000485:Allergens / Q000032:analysis*; D003718:Denmark; D004784:Environmental Monitoring / Q000379:methods*; D006801:Humans; D006967:Hypersensitivity / Q000175:diagnosis / Q000276:immunology; D007120:Immunochemistry; D006109:Poaceae / Q000276:immunology; D011058:Pollen / Q000276:immunology*; D011852:Radioallergosorbent Test; D013170:Spores; D014197:Trees / Q000276:immunology
chemical_list: D000393:Air Pollutants; D000485:Allergens