Automated high-performance liquid chromatographic method for the determination of mianserin in plasma using electrochemical detection.
An automated high-performance liquid chromatographic method for the determination of mianserin in plasma is described. Extraction and injection of the samples were automatically done by the Gilson ASPEC system using C8, 100-mg Supelclean solid-phase extraction columns. The extracts were chromatographed on a reversed-phase C18 column (150 mm x 3.9 mm I.D.) with a phosphate buffer-acetonitrile-methanol mobile phase and the analytes detected electrochemically. Calibration curves were linear to at least 53.7 ng/ml at which the between-day relative standard deviation was 5% and the recovery 101%. The limit of quantification was 1.67 ng/ml at which the between-day relative standard deviation was 9% and the recovery 92% using a sample volume of 0.5 ml. The method was applied to the determination of mianserin in the plasma of normal human volunteers participating in a comparative bioavailability study.
Brown
L W
LW
Department of Pharmacology, Faculty of Medicine, UOFS, Bloemfontein, South Africa.
Hundt
H K
HK
Swart
K J
KJ
D001682:Biological Availability; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004563:Electrochemistry / Q000379:methods; D006801:Humans; D008803:Mianserin / Q000097:blood* / Q000493:pharmacokinetics
D008803:Mianserin