title: Human serum amyloid P-component (SAP) selectively binds to immobilized or bound forms of C-reactive protein (CRP).
abstract: The two homologous human pentraxins, C-reactive protein (CRP) and serum amyloid P-component (SAP), specifically bind to each other only when the CRP is in an immobilized form bound to one of its ligands or to an antibody. CRP did not bind to immobilized SAP. The binding of SAP to immobilized forms of CRP was Ca(2+)-dependent and of sufficient affinity to occur in the presence of serum or purified serum proteins. SAP bound preferentially to a synthetic peptide corresponding to the Ca(2+)-binding region of CRP. Monoclonal antibodies to a synthetic peptide corresponding to the Ca(2+)-binding region selectively inhibited the binding interaction. Proteolytic cleavage of CRP between residues 146 and 147 within the Ca2+ binding region abolished the SAP-binding site; however, the intact subunits of the pentameric CRP were capable of binding SAP. The significance of the binding interaction is that it may serve as the basis for localization of SAP to sites of tissue damage or repair, sites where CRP is selectively deposited.
authors: Department of Microbiology, Ohio State University, Columbus 43210.
authors: S J
authors: SJ
authors: Swanson
authors: R B
authors: RB
authors: Christner
authors: R F
authors: RF
authors: Mortensen
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D002097:C-Reactive Protein / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry; D000683:Serum Amyloid P-Component / Q000378:metabolism*
chemical_list: D010455:Peptides; D000683:Serum Amyloid P-Component; D002097:C-Reactive Protein