Sex differences in the regulation of heat shock protein 70 kDa and 90 kDa in the rat ventromedial hypothalamus by estrogen.
The regulation of heat shock proteins 70 kDa (hsp 70) and 90 kDa (hsp90) by estradiol (EB) was examined in the ventromedial hypothalamus (VMH) of gonadectomized adult female and male rats with and without estradiol replacement. Estradiol significantly elevated hsp70 and hsp90 protein concentrations in EB-treated VMH of females, but not males, sacrificed at 12 h following steroid hormone compared with oil-vehicle controls. These findings confirm our previous observations that EB induces hsp70 and hsp90 in the female VMH16,17. The sex differences for the two proteins may occur by different mechanisms, since basal hsp70 levels were higher in males than females, whereas hsp90 levels were similar for the two sexes.
Olazábal
U E
UE
Lab. Neurobiology and Behavior, Rockefeller University New York, NY.
Pfaff
D W
DW
Mobbs
C V
CV
D000818:Animals; D015153:Blotting, Western; D004586:Electrophoresis; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D006360:Heat-Shock Proteins / Q000378:metabolism*; D007033:Hypothalamus, Middle / Q000378:metabolism*; D008163:Luminescent Measurements; D008297:Male; D009919:Orchiectomy; D010052:Ovariectomy; D051381:Rats; D017207:Rats, Sprague-Dawley; D012727:Sex Characteristics
D006360:Heat-Shock Proteins; D004958:Estradiol