[Anomalies produced by cytochalasin B after 10.5 days of gestation: in vitro study].
124 rats embryos (Rattus Norvegicus sp.) have been cultured for 48 hours period (from 9.5 days to 11.5 days). 24 hours after the beginning of the culture, cytochalasin B (CB) dissolved in 1% dimethylsulphoxide (DMSO) was added to the culture medium in the experimental group (61 embryos), whereas only DMSO was added to the medium in the control group (63 embryos). Survival rate in the control group was 100% whereas in CB-treated embryos the higher the dose, the lesser the survival rate. Embryo growth and morphological score was reduced in CB-treated embryos. The most common gross malformations were delays in axial rotation as well as open neural tube defects.
Mirapeix
R M
RM
Département de Sciences Morphologiques, Faculté de Médecine, Université Autonome de Barcelone, Bellaterra, Espagne.
Domenech-Mateu
J M
JM
D000014:Abnormalities, Drug-Induced / Q000209:etiology*; D000818:Animals; D046508:Culture Techniques; D003571:Cytochalasin B / Q000633:toxicity*; D004121:Dimethyl Sulfoxide; D005314:Embryonic and Fetal Development / Q000187:drug effects; D005865:Gestational Age; D051381:Rats
D003571:Cytochalasin B; D004121:Dimethyl Sulfoxide
Anomalies produites par la cytochalasine B apres 10.5 jours de gestation: etude in vitro.