Simultaneous determination of sulphadiazine and trimethoprim in plasma and tissues of cultured fish for residual and pharmacokinetic studies.
Clean-up and high-performance liquid chromatographic methods for the simultaneous determination of sulphadiazine and trimethoprim in fish plasma and tissues have been developed. The average recovery of sulphadiazine varied from 74% in liver to 92% in plasma, whereas that of trimethoprim varied from 60% in liver to 97% in plasma. The sample pretreatment procedures were simple, selective and robust, having a limit of quantification of 250 ng/ml for trimethoprim and 50 ng/ml for sulphadiazine in plasma, 15 ng/g for sulphadiazine and 80 ng/g for trimethoprim in muscle, and 30 ng/g for sulphadiazine and 160 ng/g for trimethoprim in liver. The assay was tested on plasma from Atlantic salmon treated with Tribrissen.
Hormazabal
V
V
Department of Food Hygiene, Norwegian College of Veterinary Medicine, Oslo.
Rogstad
A
A
D000818:Animals; D008099:Liver / Q000737:chemistry; D015203:Reproducibility of Results; D012474:Salmon / Q000097:blood; D013411:Sulfadiazine / Q000032:analysis* / Q000097:blood; D014295:Trimethoprim / Q000032:analysis* / Q000097:blood
D013411:Sulfadiazine; D014295:Trimethoprim