Binding of human IgA to HCl-extracted c protein from group B streptococci (GBS).
The c beta protein of group B streptococci obtained by HCl extraction appears as a ladder-like pattern in SDS-PAGE when detected by a rabbit anti-c beta serum, and a similar picture is seen when the crude extract is incubated with human IgA and an anti-human IgA conjugate. Affinity-purified c beta antigen and IgA receptors from GBS gave identical pictures in Western blots using rabbit anti-c beta serum. Both the c beta antigen and the IgA receptor are exposed on the surface of GBS as demonstrated by immunofluorescence.
Kvam
A I
AI
Department of Microbiology, Faculty of Medicine, Regional Hospital, University of Trondheim, Norway.
Iversen
O J
OJ
Bevanger
L
L
D000942:Antigens, Bacterial / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D006801:Humans; D006851:Hydrochloric Acid; D007106:Immune Sera / Q000276:immunology; D015151:Immunoblotting; D007070:Immunoglobulin A / Q000378:metabolism*; D011961:Receptors, Fc; D011971:Receptors, Immunologic / Q000032:analysis; D013292:Streptococcus agalactiae / Q000378:metabolism*
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; C045281:IgA receptor; D007106:Immune Sera; D007070:Immunoglobulin A; D011961:Receptors, Fc; D011971:Receptors, Immunologic; C070010:streptococcal beta antigen; D006851:Hydrochloric Acid