Selective enhancement of interleukin 1 beta production in myelomonocytic cell lines by insulin and its related cytokines.
The production of interleukin 1 (IL-1) by lipopolysaccharide (LPS)-stimulated myelomonocytic cell lines ML-1, THP-1 and PL-21 was significantly enhanced by the addition of insulin, insulin-like growth factor (IGF)-I or IGF-II into the cell cultures. The IL-1 activity in the supernatants from cell cultures stimulated with LPS and insulin was completely neutralized by anti-IL-1 beta antibody. Anti-IL-1 alpha antibody had no inhibitory effect. Insulin itself did not stimulate IL-1 beta production directly, but increased it in the mitogen activated cells. However, insulin had no enhancing effect on the production of IL-1 alpha by human T cell lymphotropic virus-I (HTLV-I)-infected T cell lines or on IL-2 production by mitogen-stimulated leukemia T cell lines. Thus, insulin and its related cytokines are shown here as other molecules selectively modulating the production of IL-1 beta in myelomonocytic cell lines.
Holán
V
V
Fujisaki Cell Center, Hayashibara Biochemical Laboratories, Inc., Okayama, Japan.
Minowada
J
J
D015368:Human T-lymphotropic virus 1 / Q000276:immunology; D006801:Humans; D007328:Insulin / Q000276:immunology*; D007334:Insulin-Like Growth Factor I / Q000276:immunology; D007335:Insulin-Like Growth Factor II / Q000276:immunology; D007375:Interleukin-1 / Q000096:biosynthesis*; D007376:Interleukin-2 / Q000096:biosynthesis; D008070:Lipopolysaccharides; D008213:Lymphocyte Activation; D013601:T-Lymphocytes / Q000276:immunology* / Q000382:microbiology; D014407:Tumor Cells, Cultured
D007328:Insulin; D007375:Interleukin-1; D007376:Interleukin-2; D008070:Lipopolysaccharides; D007334:Insulin-Like Growth Factor I; D007335:Insulin-Like Growth Factor II