title: Resolution of dihydroxyeicosanoates and of dihydroxyeicosatrienoates by chiral phase chromatography.
abstract: A chromatographic method is described for the direct enantiomeric characterization of 5,6-, 8,9-, 11,12-, and 14,15-vic-dihydroxyeicosatrienoic acids (DHETs), metabolites of the cytochrome P-450 arachidonate epoxygenase pathway, and of their corresponding saturated vic-dihydroxyeicosanoic acids. Following esterification, the individual methyl or pentafluorobenzyl esters are resolved by chiral-phase chromatography utilizing a Chiralcel OC or OD column. This methodology will find analytical and preparative applications since it is simple and efficient and preserves, intact, the diol functionality.
authors: Department of Medicine, Vanderbilt University Medical School, Nashville, Tennessee 37232.
authors: J H
authors: JH
authors: Capdevila
authors: S
authors: S
authors: Wei
authors: A
authors: A
authors: Kumar
authors: J
authors: J
authors: Kobayashi
authors: J R
authors: JR
authors: Snapper
authors: D C
authors: DC
authors: Zeldin
authors: R K
authors: RK
authors: Bhatt
authors: J R
authors: JR
authors: Falck
mesh_terms: D015126:8,11,14-Eicosatrienoic Acid / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D000090762:Cytochrome P-450 CYP2J2; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D007202:Indicators and Reagents; D007536:Isomerism; D013058:Mass Spectrometry / Q000379:methods; D010105:Oxygenases / Q000378:metabolism
chemical_list: D007202:Indicators and Reagents; D003577:Cytochrome P-450 Enzyme System; D010105:Oxygenases; D000090762:Cytochrome P-450 CYP2J2; D015126:8,11,14-Eicosatrienoic Acid