Immunohistochemical study on the distribution of sarcoplasmic reticulum calcium ATPase in various human tissues using novel monoclonal antibodies.
Novel monoclonal antibodies were raised against sarcoplasmic reticulum calcium (Ca2+)-ATPase of human skeletal muscle. Immunohistochemical analysis demonstrated that these antibodies, designated 6F5 and 7F10, bind Ca(2+)-ATPase of non-muscle tissue of the adult including parathyroid, islets of Langerhans, anterior lobe of the pituitary gland and photoreceptor cells of the retina as well as skeletal muscle. A positive reaction was also found for fetal tissues including skeletal muscle, heart, chondrocytes and peripheral nerves. Our results for distribution suggest that Ca(2+)-ATPase is strongly expressed in the tissues and cells in which signal transduction is actively carried out by Ca2+ release from the cytoplasmic Ca2+ pool.
Kuroda
M
M
Department of Pathology, Faculty of Medicine, University of Tokyo, Japan.
Horiuchi
H
H
Ono
A
A
Kawakita
M
M
Oka
T
T
Machinami
R
R
D000911:Antibodies, Monoclonal; D000252:Calcium-Transporting ATPases / Q000276:immunology / Q000378:metabolism*; D002356:Cartilage / Q000378:metabolism; D003429:Cross Reactions; D005333:Fetus / Q000033:anatomy & histology / Q000378:metabolism; D005786:Gene Expression Regulation; D006801:Humans; D007515:Islets of Langerhans / Q000378:metabolism; D010280:Parathyroid Glands / Q000378:metabolism; D010903:Pituitary Gland, Anterior / Q000378:metabolism; D012160:Retina / Q000378:metabolism; D012519:Sarcoplasmic Reticulum / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D000252:Calcium-Transporting ATPases