title: Determination of human mast cell tryptase by bioluminescence-enhanced two-site immunometric assay.
abstract: A highly sensitive bioluminescence-enhanced two-site immunometric assay for human mast cell tryptase was developed. The usable range of the standard curve was from 10 ng to 10 micrograms per litre. The intra-assay coefficient of variation of the test was between 8.0 and 12.3%, and the inter-assay coefficient of variation between 8.3 and 13.5%. Recovery of human mast cell tryptase in human serum was 100% (fraction of serum in the test sample: 0.30).
authors: Medor GmbH, Herrsching, Bundesrepublik Deutschland.
authors: E
authors: E
authors: Schneider
authors: D
authors: D
authors: Gabrijelcic
authors: R
authors: R
authors: Geiger
mesh_terms: D002021:Buffers; D053818:Chymases; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D008163:Luminescent Measurements; D008407:Mast Cells / Q000201:enzymology*; D012697:Serine Endopeptidases / Q000378:metabolism*; D053802:Tryptases
chemical_list: D002021:Buffers; D012697:Serine Endopeptidases; C083294:chymase 2; D053818:Chymases; D053802:Tryptases