Detection of abnormal haemoglobin by capillary electrophoresis and structural identification.
Haemoglobin obtained from a male adult Ghanian with retinopathy, which was probably caused by haemoglobinopathy was analysed by capillary electrophoresis (CE) for clinical diagnosis. Two major peaks, which were in the ratio of nearly one, were detected. The elution times of these peaks (HbXI and HbXII) were faster than that of normal haemoglobin (HbA). The existence of two different abnormal types of haemoglobin was clear in the patient blood. The following sequence analysis revealed that the first peak (HbXI) was HbC and the second (HbXII) was HbS on the electropherogram, and that the patient was a heterozygote of HbS and HbC (HbSC disease). One of the diagnostic processes in a haemoglobin disease was shown by the combined use of CE, HPLC and a protein sequencer.
Ishioka
N
N
Division of Biochemistry, Jikei University School of Medicine, Tokyo, Japan.
Iyori
N
N
Noji
J
J
Kurioka
S
S
D000328:Adult; D000595:Amino Acid Sequence; D002197:Capillary Action; D004586:Electrophoresis / Q000379:methods*; D005869:Ghana / Q000208:ethnology; D005914:Globins / Q000737:chemistry; D006444:Hemoglobin C / Q000032:analysis* / Q000737:chemistry; D006450:Hemoglobin SC Disease / Q000097:blood*; D006451:Hemoglobin, Sickle / Q000032:analysis* / Q000737:chemistry; D006579:Heterozygote; D006801:Humans; D007564:Japan; D008297:Male; D008969:Molecular Sequence Data; D010449:Peptide Mapping; D012164:Retinal Diseases / Q000097:blood*
D006451:Hemoglobin, Sickle; D005914:Globins; D006444:Hemoglobin C