RGD-albumin conjugate: expression of tissue regeneration activity.
Albumin conjugated with Arg-Gly-Asp (RGD) has cellular adhesive activity comparable to that of fibronectin in vitro. This study examined whether RGD-albumin conjugate (RGD-ALB) can promote soft tissue ingrowth into a porous matrix in vivo. Polyurethane sponges with 150-500 microns pore size adsorbed with RGD-ALB, fibronectin and albumin were implanted in the subcutaneous tissue of rats. The RGD-ALB-adsorbed sponge exhibited tissue ingrowth comparable to that of the fibronectin-adsorbed sponge at the early period of implantation. On the other hand, the tissue ingrowth was markedly retarded for non-adsorbed and albumin-adsorbed sponges. The enhanced tissue ingrowth found for RGD-ALB and fibronectin was deduced to be mainly a result of its cell-adhesion activity. It is concluded that RGD-ALB exhibits tissue ingrowth-promoting activity at the initial stage of wound healing as effectively as fibronectin.
Kishida
A
A
Department of Bioengineering, National Cardiovascular Center Research Institute, Osaka, Japan.
Takatsuka
M
M
Matsuda
T
T
D000418:Albumins / Q000494:pharmacology*; D000818:Animals; D005353:Fibronectins / Q000494:pharmacology*; D009842:Oligopeptides / Q000494:pharmacology*; D019736:Prostheses and Implants; D051381:Rats; D017208:Rats, Wistar; D014945:Wound Healing / Q000187:drug effects*
D000418:Albumins; D005353:Fibronectins; D009842:Oligopeptides; C079064:RGD-albumin conjugate