title: Phenotypes and alloantigen-presenting activity of individual clones of microglia derived from the mouse brain.
abstract: To clarify the origin and function of the microglia residing in the central nervous system, we cloned brain cells from newborn and adult mice in soft agar containing the macrophage-specific growth factor, colony-stimulating factor-1 and expanded the cells from individual colonies in liquid culture medium. The results of molecular, immunophenotypic and functional analyses showed that the clones consisted of microglia derived from the macrophage family of cells. For instance, the microglia contain mRNA transcripts for the receptor for colony-stimulating factor-1 and truncated CD4 transcripts similar to those found in mouse macrophages but not T helper cells. About a third of the microglial progenitors gave rise to progeny that constitutively induced the selective proliferation of naive allogeneic CD8+ T cells in a CD4+ T cell-independent manner, a response that was inhibited by monoclonal antibodies to major histocompatibility complex (MHC) class I molecules on the microglia. Since all microglia expressed similar levels of MHC class I molecules, the basis for the alloantigen presentation likely resides in the ability of some clones of microglia to synthesize co-stimulator molecules that are required for CD8+ T cell proliferation. Thus, at least some microglia in mouse brain arise from endogenous progenitors and appear capable of specialized functions.
authors: Department of Immunology, St. Jude Children's Research Hospital, Memphis, TN 38101-0318.
authors: S C
authors: SC
authors: Moore
authors: J M
authors: JM
authors: McCormack
authors: E
authors: E
authors: Armendariz
authors: J
authors: J
authors: Gatewood
authors: W S
authors: WS
authors: Walker
mesh_terms: D000818:Animals; D000938:Antigen-Presenting Cells / Q000502:physiology*; D001483:Base Sequence; D001921:Brain / Q000276:immunology*; D015704:CD4 Antigens / Q000235:genetics; D002999:Clone Cells; D004247:DNA / Q000032:analysis; D005260:Female; D016130:Immunophenotyping; D007377:Interleukin-3 / Q000494:pharmacology; D007519:Isoantigens / Q000276:immunology*; D016173:Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D009457:Neuroglia / Q000276:immunology*; D016186:Receptor, Macrophage Colony-Stimulating Factor / Q000235:genetics
chemical_list: D015704:CD4 Antigens; D007377:Interleukin-3; D007519:Isoantigens; D016173:Macrophage Colony-Stimulating Factor; D004247:DNA; D016186:Receptor, Macrophage Colony-Stimulating Factor