title: Erythropoietin levels in amniotic fluid and extraembryonic coelomic fluid in the first trimester of pregnancy.
abstract: OBJECTIVE
  The aim was to measure erythropoietin levels in amniotic fluid and extraembryonic coelomic fluid from 7-12 weeks' gestation.
  
  
  METHODS
  Twenty healthy women with ultrasonographically normal first trimester pregnancies prior to surgical termination.
  
  
  METHODS
  Paired samples of amniotic fluid and extraembryonic coelomic fluid were collected by transvaginal ultrasound guided needling. Erythropoietin was measured in both pregnancy fluids using a radioimmunoassay.
  
  
  RESULTS
  There was a highly significant difference between erythropoietin levels in extraembryonic coelomic fluid (median level 15.45 mU/ml; range 6.8-32.1 mU/ml) and those in amniotic fluid (median 5.0 mU/ml; range < 5.0-5.8 mU/ml) (P < 0.0001; Mann-Whitney U-test). The levels of erythropoietin in maternal serum (median 15.4 mU/ml; range 5.6-29.4 mU/ml) were similar to those in the extra-embryonic coelom (P = 0.81; Mann-Whitney U-test). No relation was demonstrated between erythropoietin levels in amniotic fluid or coelomic fluid and stage of gestation.
  
  
  CONCLUSIONS
  High levels of erythropoietin in coelomic fluid suggests that the hormone is involved in the process of human extraembryonic erythropoiesis. The exact regulatory role remains unknown.
authors: Department of Obstetrics, St Bartholomew's Hospital, London, UK.
authors: J
authors: J
authors: Campbell
authors: N
authors: N
authors: Wathen
authors: M
authors: M
authors: Lewis
authors: H
authors: H
authors: Fingerova
authors: T
authors: T
authors: Chard
mesh_terms: D000653:Amniotic Fluid / Q000737:chemistry*; D004921:Erythropoietin / Q000032:analysis*; D005260:Female; D005865:Gestational Age; D006801:Humans; D011247:Pregnancy; D011261:Pregnancy Trimester, First; D015017:Yolk Sac / Q000737:chemistry*
chemical_list: D004921:Erythropoietin