Synthesis of specific peptide substrates for HIV-1 proteinase.
Two small peptide substrates for HIV-1 proteinase were synthesised. The sequences chosen were basically from that of the gag-pol protein, which is the natural substrate for the proteinase. To protect these peptides from the attack of exopeptidases, the N- and C-termini were suitably protected, which also makes these substrates specific to HIV-proteinase and eliminates the requirement for highly purified enzyme.
Handa
B K
BK
Department of Physical Chemistry, Roche Products Limited, Welwyn Garden City, Hertfordshire, England.
Kay
C
C
D000595:Amino Acid Sequence; D002625:Chemistry, Organic / Q000379:methods; D015685:Fusion Proteins, gag-pol / Q000378:metabolism*; D016333:HIV Protease / Q000378:metabolism*; D015497:HIV-1 / Q000201:enzymology*; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis* / Q000302:isolation & purification / Q000378:metabolism*
D015685:Fusion Proteins, gag-pol; D010455:Peptides; D016333:HIV Protease