Expression of the pS2 gene in breast tissues assessed by pS2-mRNA analysis and pS2-protein radioimmunoassay.
The expression of the pS2 gene in breast tissues was assessed by measuring pS2-protein using a radioimmunoassay, and by determining pS2-mRNA using Northern blotting. There was a good correlation between the two measurements, indicating that expression of the pS2 gene in breast tissues may be assessed by either method. Since radioimmunoassay is technically easier and more efficient than Northern blotting, radioimmunoassay will be the method of choice in routine applications.
Hähnel
E
E
Department of Pathology, University of Western Australia, Queen Elizabeth II Medical Centre, Nedlands.
Robbins
P
P
Harvey
J
J
Sterrett
G
G
Hähnel
R
R
D015152:Blotting, Northern; D001943:Breast Neoplasms / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D012333:RNA, Messenger / Q000032:analysis*; D011863:Radioimmunoassay
D012333:RNA, Messenger