title: Evidence for retrotranscription of protein-coding genes in the Drosophila subobscura genome.
abstract: Evidence is provided for the presence of retrosequences (also named retroposons) arising from Adh in the Drosophila subobscura genome. Restriction analysis and primary structure of two different retrosequence-containing clones, S812 and S135, are reported. The fact that these retrosequences lack introns and a recognizable promoter strongly supports their retrotranscriptional origin. Adjacent to the two retrosequences analyzed, a middle repetitive DNA element has been found which bears no clear similarity to any sequence reported to date in the GenBank/EMBL Data Library. A comparative analysis of these retrosequences with the functional Adh gene of D. subobscura is presented. In addition, a model concerning the origin, functionality, and propagation of these genome elements is discussed.
authors: Departament de Genètica, Facultat de Biologia, Universitat de Barcelona, Spain.
authors: G
authors: G
authors: Marfany
authors: R
authors: R
authors: Gonzàlez-Duarte
mesh_terms: D000426:Alcohol Dehydrogenase / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D005075:Biological Evolution; D015139:Blotting, Southern; D004247:DNA; D004330:Drosophila / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011506:Proteins / Q000235:genetics*; D011544:Pseudogenes; D015183:Restriction Mapping; D014158:Transcription, Genetic
chemical_list: D011506:Proteins; D004247:DNA; D000426:Alcohol Dehydrogenase