[Characterization of murine monoclonal antibodies against fetal erythrocytes].
Balb/c mice were immunized against papain-treated fetal erythrocytes and splenocytes were fused with Sp2/0-Ag-14 myeloma cells. Several hybrids secreting antibodies directed against antigenic determinants predominantly exposed on fetal and cord cells were selected and cloned twice. Antibodies NaM61-1A2 and NaM61-768 (IgM class) were shown to be specific for an endo-beta-galactosidase-sensitive oligosaccharide chain. The antigen, strongly expressed on fetal and cord cells, was identified as the i blood group antigen. The antibodies represent powerful blood group reagents to be use in conventional agglutination techniques as well as in the gel typing system and in indirect flow cytometry. The antibody NaM46-4A8 (IgG class) is specific for an antigenic structure expressed on fetal cells and accessible only after papain, ficin, bromelin and endo-beta galactosidase treatment. The antigen was not identified.
Blanchard
D
D
Centre Régional de Transfusion Sanguine, Nantes.
Bernard
D
D
Loirat
M J
MJ
Frioux
Y
Y
Guimbretière
J
J
Guimbretière
L
L
D000328:Adult; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D001963:Bromelains / Q000494:pharmacology; D002240:Carbohydrate Sequence; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000276:immunology*; D005260:Female; D005312:Fetal Blood / Q000276:immunology*; D005361:Ficain / Q000494:pharmacology; D005434:Flow Cytometry; D006026:Glycoside Hydrolases; D006386:Hemagglutination Tests; D006388:Hemagglutinins / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology*; D007075:Immunoglobulin M / Q000276:immunology*; D007231:Infant, Newborn / Q000097:blood; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology; D008969:Molecular Sequence Data; D010206:Papain / Q000494:pharmacology; D011247:Pregnancy; D001616:beta-Galactosidase / Q000494:pharmacology
D000911:Antibodies, Monoclonal; D006388:Hemagglutinins; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D001963:Bromelains; D006026:Glycoside Hydrolases; C026093:keratan-sulfate endo-1,4-beta-galactosidase; D001616:beta-Galactosidase; D010206:Papain; D005361:Ficain
Caractérisation d'anticorps monoclonaux murins dirigés contre les érythrocytes foetaux.