The existence of a soluble plasmalogenase in guinea pig tissues.
The distribution of plasmalogenase for the hydrolysis of 1-alkenyl-2-acyl-sn-glycero-3-phosphoethanolamine (plasmenylethanolamine) in the subcellular fractions of guinea pig tissues was examined. Plasmalogenase activity was found in high abundance in the cytosolic fractions of the brain and the heart. Assessment of microsomal marker enzyme activities in the cytosolic fraction revealed that plasmalogenase activity in the cytosol was not due to microsomal contaminations. The characteristics of the cytosolic plasmalogenase were very similar to the microsomal enzyme with respect to the pH profile of the reaction, the presence of divalent cations and Km values for plasmenylethanolamine. However, the cytosolic enzyme was slightly less stable at 55 degrees C than the microsomal enzyme. Cytosolic enzyme activity was eluted as a broad peak in Sepharose 6B chromatography with an average molecular weight of 250,000. Our results demonstrate that most of brain plasmalogenase activity is soluble which makes the brain cytosol an excellent source to initiate the purification of this enzyme.
McMaster
C R
CR
Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Manitoba, Winnipeg, Canada.
Lu
C Q
CQ
Choy
P C
PC
D000818:Animals; D001921:Brain / Q000201:enzymology; D002412:Cations / Q000494:pharmacology; D003600:Cytosol / Q000201:enzymology; D004492:Edetic Acid / Q000494:pharmacology; D006168:Guinea Pigs; D006863:Hydrogen-Ion Concentration; D006867:Hydrolases / Q000032:analysis* / Q000187:drug effects / Q000302:isolation & purification; D008099:Liver / Q000201:enzymology; D008861:Microsomes / Q000201:enzymology; D009206:Myocardium / Q000201:enzymology; D010955:Plasmalogens / Q000378:metabolism; D011489:Protein Denaturation; D013347:Subcellular Fractions / Q000201:enzymology*; D014018:Tissue Distribution
D002412:Cations; D010955:Plasmalogens; C020791:phosphatidal ethanolamines; D004492:Edetic Acid; D006867:Hydrolases; C022403:plasmalogenase