[Cloning and sequence analysis of salmon growth hormone cDNA].
cDNA clones encoding Chinook Salmon, Oncorhynchus tschawytscha, growth hormone (sGH) have been isolated from a cDNA library prepared from Chinook Salmon pituitary gland poly(A)+ RNA. Synthetic oligodeoxynucleotide mixtures based on amino acid residues 1-7 of sGH and 166-172 of sGH were used as hybridization probes to select recombinant plasmids carrying the sGH coding sequence. The complete nucleotide sequence of sGH cDNA has been determined. The cDNA sequence codes for a polypeptide of 210 amino acids including a putative signal sequence of 22 amino acids. The 5' and 3' untranslated regions of the message were 70 and 446 bases long, respectively. Comparison of the nucleotide sequence and amino acid sequence between Chum sGH and Chinook sGH indicated that there were 97% and 99% homology respectively.
Song
S
S
Dept. of Medicine, Second Teaching Hospital, Tianjin Medical College.
Trinh
K T
KT
Hew
C L
CL
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis / Q000096:biosynthesis*; D013006:Growth Hormone / Q000737:chemistry / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D051381:Rats; D012474:Salmon / Q000235:genetics*
D013006:Growth Hormone; D004247:DNA