title: [Site-specific photomodification of nucleic acids with arylazide and perfluoroarylazide oligonucleotide derivatives. II. Specificity in relation to nucleosides].
abstract: Oligonucleotide reagents bearing aromatic azido groups of different structures were shown to be suitable for nucleoside specific photomodification of nucleic acids. Modification of the pentadecanucleotide targets d(TAAGTGGAGTTTGGC), d(TAAGTGGAAAAAAAA), d(TAAGTGGACCCCCCC) and d(TAAGTGGATTTTTTT) was investigated with reagents d(UCH2OCH2CH2NHCORCCACTT) carrying a photoactive group R(R1-n-azidotetrafluorophenyl-reagent (I), R2-2-nitro-5-azidophenyl-reagent (II) and R3-n-azidophenyl-reagent (III)) at C-5-modified deoxyuridine. Photomodification did not exceed 5% for the targets in case of reagent (III); the modification extent was 25-50% depending on the target sequence for reagent (II); reagent (I) with perfluoro azido group was the most effective, that provided 60-70% of modification. Reagents (I) and (II) were found to be sensitive to the nucleoside sequence of the target: the most vulnerable sites for reagent (I) and (II) were guanine and cytosine residues, respectively. These bases were modified predominantly when being adjacent to the addressed site of the target.
authors: M I
authors: MI
authors: Dobrikov
authors: V V
authors: VV
authors: Gorn
authors: V F
authors: VF
authors: Zarytova
authors: A S
authors: AS
authors: Levina
authors: T A
authors: TA
authors: Prikhod'ko
authors: G V
authors: GV
authors: Shishkin
authors: D R
authors: DR
authors: Tabatadze
authors: M M
authors: MM
authors: Zaalishvili
mesh_terms: D001345:Autoradiography; D001386:Azides / Q000737:chemistry*; D001483:Base Sequence; D008969:Molecular Sequence Data; D009696:Nucleic Acids / Q000737:chemistry*; D009841:Oligonucleotides; D010777:Photochemistry
chemical_list: D001386:Azides; D009696:Nucleic Acids; D009841:Oligonucleotides
vernacular_title: Komplementarno adresovannaia fotomodifikatsiia nukleinovykh kislot arilazidnymi i perftorarilazidnymi proizvodnymi oligonukleotidov. II. Issledovanie spetsifichnosti po otnosheniiu k nukleozidam.