Biomolecular regulation of the IgE immune response. I. Cell-associated IgE and in vitro IgE synthesis.
Several cell types, including mast cells, basophils, macrophages/monocytes, lymphocytes, platelets and eosinophils, may bind or contain IgE. To investigate the source of cell-associated IgE and its relation to spontaneous IgE synthesis, peripheral blood mononuclear cells from allergic and non-allergic donors were examined. Using a combination of different cell fractionation techniques and varying methods for extraction of cell-associated IgE, data were obtained indicating that monocytes constitute a major source of cell-associated IgE in human blood. The amount of cell-associated IgE in peripheral blood mononuclear cells from allergic and non-allergic donors varied more than 100-fold but correlated closely to the level of serum IgE (r = 0.84, p < 0.001, n = 38). Spontaneous and mitogen-induced in vitro syntheses of IgA, IgE and IgG were compared for allergic and non-allergic donors. Only one donor with very high serum IgE demonstrated spontaneous or mitogen-induced in vitro IgE synthesis despite synthesis of IgA and IgG.
Poulsen
L K
LK
Laboratory of Medical Allergology, National University Hospital, Copenhagen, Denmark.
Baron
L
L
Heinig
J H
JH
Stahl Skov
P
P
Bendtzen
K
K
D001249:Asthma / Q000097:blood / Q000276:immunology*; D002452:Cell Count; D002458:Cell Fractionation; D002478:Cells, Cultured; D006801:Humans; D007111:Immunity, Cellular; D007070:Immunoglobulin A / Q000032:analysis; D007073:Immunoglobulin E / Q000032:analysis / Q000096:biosynthesis*; D007074:Immunoglobulin G / Q000032:analysis; D015592:Immunoradiometric Assay; D009000:Monocytes / Q000276:immunology; D012220:Rhinitis / Q000097:blood / Q000276:immunology*
D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007073:Immunoglobulin E