PCR-SSCP: a method for detection of mutations.
PCR-SSCP (polymerase chain reaction-single-strand conformation polymorphism) analysis is one of the simplest and perhaps one of the most sensitive methods for detection of mutations based on PCR technology. The principles of PCR-SSCP, guidelines for experiments, and applications of this technique in various fields are reviewed.
Hayashi
K
K
Oncogene Division, National Cancer Center Research Institute, Tokyo, Japan.
D001419:Bacteria / Q000235:genetics; D004252:DNA Mutational Analysis / Q000379:methods*; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D004277:DNA, Single-Stranded / Q000032:analysis / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005188:False Negative Reactions; D030342:Genetic Diseases, Inborn / Q000235:genetics; D005819:Genetic Markers; D006801:Humans; D009369:Neoplasms / Q000235:genetics; D009690:Nucleic Acid Conformation; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012091:Repetitive Sequences, Nucleic Acid; D012680:Sensitivity and Specificity
D004269:DNA, Bacterial; D004277:DNA, Single-Stranded; D005819:Genetic Markers