Ca2+-ATPase in mucous and oxyntico-peptic cells of the fowl proventriculus.
Calcium adenosine triphosphatase (Ca(2+)-ATPase) was localized by means of histo-and ultracytochemistry in the secretory cells of the proventriculus of the domestic fowl. The mucous cells exhibited plasmalemmal-associated enzyme activity on the external aspect of the basolateral cell membrane. Intracellularly, the luminal aspect of Golgi-membranes and of secretory vesicle membranes reacted positively for Ca(2+)-ATPase activity, as did the apical cytosol and the matrix of lysosomes. Oxyntico-peptic cells were characterized by apical and apico-lateral plasmalemmal activity and by an organelle-associated distributional pattern similar to that in the mucous cells. In addition, Ca(2+)-ATPase was associated either with the matrix of mitochondria or with tubuli of the rough-surfaced endoplasmic reticulum. The results are discussed with respect to messenger and effector functions of calcium in the process of proventricular mucus secretion. In addition, Ca(2+)-ATPase distributional patterns in the oxyntico-peptic cell are related to the unique structure and function of these cells.
Salem
A O
AO
Institute fur Veterinaranatomie, -Histologie und -Embryologie der Universitat, Giessen, FRG.
Kressin
M
M
Schnorr
B
B
D000818:Animals; D001717:Birds / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism; D000252:Calcium-Transporting ATPases / Q000032:analysis*; D005753:Gastric Mucosa / Q000166:cytology / Q000201:enzymology* / Q000648:ultrastructure; D010295:Parietal Cells, Gastric / Q000166:cytology / Q000201:enzymology* / Q000648:ultrastructure
D000252:Calcium-Transporting ATPases; D002118:Calcium