Human immunodeficiency virus type 1-infected HL-60 cells are capable of both monocytic and granulocytic differentiation.
We have used the human myelomonocytic cell line HL-60 as a model system to determine whether human immunodeficiency virus type 1 (HIV-1) infection affects differentiation of myeloid progenitor cells. HL-60 cells were infected with three HIV-1 isolates (IIIB, NL4-3 and PM213). HIV-1 antigen expression and cytopathicity in HL-60 cells infected with each of the three isolates was delayed by approximately 15 days as compared to those in the prototypic T cell line, H9. Chronically infected HL-60 cells and clonal lines derived from them were treated with dimethyl formamide (DMF) and induced to differentiate into granulocytes. Approximately the same percentage of these cells as of DMF-treated, uninfected HL-60 cells differentiated. Superoxide production by infected and uninfected DMF-induced cells was similar. Likewise, approximately the same percentage of cells in infected and uninfected cultures became adherent and were positive for non-specific esterase when monocytic differentiation was induced. The data demonstrate that HL-60 cells infected with HIV-1 are capable of morphological and functional granulocytic and monocytic differentiation.
Pise
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Center for Virology, Children's National Medical Center, Washington, D.C. 20010.
Newburger
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Holland
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D015704:CD4 Antigens / Q000378:metabolism; D002454:Cell Differentiation; D003588:Cytopathogenic Effect, Viral; D006098:Granulocytes / Q000166:cytology*; D015658:HIV Infections / Q000473:pathology*; D015497:HIV-1 / Q000254:growth & development*; D006410:Hematopoiesis; D006801:Humans; D066298:In Vitro Techniques; D009000:Monocytes / Q000166:cytology*; D014407:Tumor Cells, Cultured
D015704:CD4 Antigens