title: Determination of vitamin C and organic acids in various fruits by HPLC.
abstract: Liquid chromatographic methodology for determination of vitamin C and organic acids in different fruits (whortleberry, blackberry, red currant, black currant, raspberry, babaco, feijoa, kiwano, passion fruit, red and yellow tamarillos, medlar, and persimmon) cultivated in Galicia, Spain are developed. Both methods use a C18 column and an RP-18 precolumn for the stationary phase. High-performance-liquid-chromatographic-(HPLC-) grade water is brought to pH 2.2 with sulphuric acid for the mobile phase. The flow rate is 0.4 mL/min, and UV detection is at 254 nm for vitamin C and 214 nm for organic acids. Coefficients of variation of 1.84, 1.20, 1.00, and 2.60% are obtained for the vitamin C, quinic acid, malic acid and citric acid, respectively.
authors: Departamento de Química Analítica, Nutrición y Bromatología, Facultad de Farmacia, La Coruña, Spain.
authors: M A
authors: MA
authors: Romero Rodriguez
authors: M L
authors: ML
authors: Vazquez Oderiz
authors: J
authors: J
authors: Lopez Hernandez
authors: J
authors: J
authors: Simal Lozano
mesh_terms: D001205:Ascorbic Acid / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002951:Citrates / Q000032:analysis*; D005638:Fruit / Q000737:chemistry*; D008293:Malates / Q000032:analysis*; D011801:Quinic Acid / Q000032:analysis*
chemical_list: D002951:Citrates; D008293:Malates; D011801:Quinic Acid; D001205:Ascorbic Acid