Long-term expression of a retrovirally introduced beta-galactosidase gene in rodent cells implanted in vivo using biodegradable polymer meshes.
Grafts of various types of cells have been performed using bioresorbable polymer matrices. These synthetic fibers are degraded by hydrolysis into normal metabolic intermediates and induce a number of events that are conductive to healing and/or repair, the most important of which may be angiogenesis. The use of biodegradable meshes to deliver genetically altered cells was studied. A beta-galactosidase gene was inserted into Long-Evans rat bone marrow stromal (BMS) cells or fibroblasts derived from C57BL/6J mouse embryos using the retroviral vector LNL-SLX beta gal. Expression was monitored using X-gal staining. X-gal+ cells from monolayer cultures were seeded onto either polyglycolic acid (PGA) or polyglactin (PGL) biodegradable meshes and grown to confluence. Two types of grafts were performed: (1) embryonic C57BL/6J mouse fibroblasts (EMF) into either nude mice or adult C57BL/6J mice, and (2) Long-Evans rat BMS into Long-Evans rats. Beta-Galactosidase activity was found for up to 152 days for EMF in nude mice, 123 days for EMF in adult C57BL/6J mice, and 90 days for grafts of syngeneic BMS cells into Long-Evans rats. Noninfected cells grafted using the same methods did not stain with X-gal.
Naughton
B A
BA
Medical Laboratory Sciences Department, Hunter College School of Health Sciences, New York, New York.
Dai
Y
Y
Sibanda
B
B
Scharfmann
R
R
San Roman
J
J
Zeigler
F
F
Verma
I M
IM
D000818:Animals; D001854:Bone Marrow Cells; D015870:Gene Expression; D005822:Genetic Vectors; D011108:Polymers; D051381:Rats; D012190:Retroviridae / Q000235:genetics; D013630:Tampons, Surgical; D013762:Tetrahydrofolate Dehydrogenase / Q000235:genetics; D013997:Time Factors; D014162:Transfection / Q000379:methods*; D001616:beta-Galactosidase / Q000235:genetics
D011108:Polymers; D013762:Tetrahydrofolate Dehydrogenase; D001616:beta-Galactosidase