Antibody response in Plasmodium vinckei malaria after treatment with chloroquine and adjuvant interferon-gamma.
The antibody response of mice infected with Plasmodium vinckei after treatment with chloroquine either alone or in combination with interferon-gamma (IFN-gamma) was determined. Sequential serum samples were drawn from BALB/c mice receiving either 240 micrograms chloroquine on the day of infection or 120 micrograms chloroquine plus 10(4) units IFN-gamma daily for 11 days beginning on day 3 prior to infection. Mice treated with additional IFN-gamma showed an early induction of IgG2a response and a reduction in IgG1 antibodies as detected by the immunofluorescence technique at between 10 and 16 days after infection as compared with mice treated with chloroquine alone. Thus, IFN-gamma may partly exert its antimalarial activity via the induction of IgG2a antibody formation. At 4-6 weeks after infection, when mice from both groups resisted homologous re-infection, the predominant antibody isotypes found in both groups were IgG1 and IgG2a. Serum samples obtained from mice in both treatment groups at 6 weeks after infection were used for serum transfer experiments. When parasitised erythrocytes were preincubated with such immune serum, a retardation of the course of parasitaemia by 2 days was observed.
Finnemann
S
S
Landesinstitut für Tropenmedizin Berlin, Federal Republic of Germany.
Kremsner
P G
PG
Chaves
M F
MF
Schumacher
C
C
Neifer
S
S
Bienzle
U
U
D000818:Animals; D000913:Antibodies, Protozoan / Q000096:biosynthesis*; D017024:Chemotherapy, Adjuvant; D002738:Chloroquine / Q000627:therapeutic use*; D005260:Female; D005455:Fluorescent Antibody Technique; D007116:Immunization, Passive; D015151:Immunoblotting; D007074:Immunoglobulin G / Q000096:biosynthesis; D007075:Immunoglobulin M / Q000096:biosynthesis; D007371:Interferon-gamma / Q000627:therapeutic use*; D008288:Malaria / Q000276:immunology* / Q000517:prevention & control / Q000628:therapy; D051379:Mice; D010961:Plasmodium / Q000276:immunology*; D011994:Recombinant Proteins
D000913:Antibodies, Protozoan; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D011994:Recombinant Proteins; D007371:Interferon-gamma; D002738:Chloroquine