[Free radical damage to chondrocytes and protection by selenium, tocopherol and ascorbic acid using serum-free medium method].
Free radical derived from xanthine oxidase damage to rabbit articular chondrocytes cultured in serum-free medium and antioxidant defense factors protecting chondrocytes from free radical were studied. The results showed that free radical mediated an inhibition of DNA as well as proteoglycan synthesis in cultured chondrocytes and selenium, a-tocopherol and L-ascorbic acid failed to protect chondrocytes from free radicals.
Wei
X Q
XQ
Xian Medical University.
D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology*; D002358:Cartilage, Articular / Q000166:cytology*; D002478:Cells, Cultured; D016895:Culture Media, Serum-Free; D004247:DNA / Q000096:biosynthesis; D005609:Free Radicals; D011509:Proteoglycans / Q000096:biosynthesis*; D011817:Rabbits; D012643:Selenium / Q000494:pharmacology*; D014810:Vitamin E / Q000494:pharmacology; D014969:Xanthine Oxidase / Q000494:pharmacology; D014970:Xanthines / Q000494:pharmacology
D016895:Culture Media, Serum-Free; D005609:Free Radicals; D011509:Proteoglycans; D014970:Xanthines; D014810:Vitamin E; D004247:DNA; D014969:Xanthine Oxidase; D012643:Selenium; D001205:Ascorbic Acid