Growth activity of bovid herpesvirus 1 in bovine follicular oocytes with cumulus cells.
Bovine follicular oocytes collected from bovine ovaries were exposed to bovid herpesvirus 1 (BHV-1). After washings, these oocytes were cultured to mature. As a result BHV-1 could not be removed from the oocytes and could replicate in the oocytes with cumulus cells, but not in the oocytes without the cells. Moreover, the specific fluorescence for BHV-1 was detected in the cumulus cells by a indirect immunofluorescent technique. Therefore these findings suggested BHV-1 could be absorbed in the oocytes but the replication of BHV-1 was done in the cumulus cells.
Tsuboi
T
T
Tohoku Branch Laboratory, National Institute of Animal Health, Aomori, Japan.
Kanazawa
Y
Y
Syoji
T
T
Tokuhisa
S
S
D000818:Animals; D002417:Cattle; D005260:Female; D006564:Herpesviridae / Q000254:growth & development* / Q000302:isolation & purification; D009865:Oocytes / Q000382:microbiology*; D006080:Ovarian Follicle / Q000166:cytology*; D014779:Virus Replication