Immunoassays for the determination of human tissue kallikrein (TK) in different body fluids based on monoclonal antibodies.
A monoclonal antibody produced against human tissue kallikrein was used to develop solid phase immunoassays for the determination of total immunoreactive tissue kallikrein, of the complex of tissue kallikrein with alpha 1-proteinase inhibitor, and of enzymatically active tissue kallikrein. The assays permit the specific determination of various forms of tissue kallikrein in body fluids and should be very useful in studies on the biological function of tissue kallikrein-kinin systems.
Witzgall
K
K
Department of Clinical Chemistry and Clinical Biochemistry, University of Munich, Germany.
Godec
G
G
Shimamoto
K
K
Fink
E
E
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal; D000941:Antigens / Q000032:analysis; D001826:Body Fluids / Q000201:enzymology; D002863:Chromogenic Compounds; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D007610:Kallikreins / Q000032:analysis* / Q000276:immunology* / Q000652:urine; D008297:Male; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000737:chemistry; D012463:Saliva / Q000201:enzymology; D012661:Semen / Q000201:enzymology; D013379:Substrate Specificity
D000911:Antibodies, Monoclonal; D000941:Antigens; D002863:Chromogenic Compounds; D009842:Oligopeptides; C031006:Val-Leu-Arg-p-nitroanilide; D007610:Kallikreins