In vitro antigen-specific IgE response is refractory to suppression by interferon-gamma.
Although interferon (IFN)-gamma has been shown to be involved in the down-regulation of polyclonal IgE response in murine B cells that were activated by lipopolysaccharide (LPS) and interleukin 4 (IL4), effects of IFN-gamma on antigen-specific IgE responses have not been fully investigated. We have developed the following culture systems for inducing antigen-specific IgE responses in murine lymphocytes, and examined the effects of IFN-gamma on the following responses in vitro. (1) Anti-trinitrophenyl (TNP) IgE response induced by the stimulation with TNP-keyhole limpet hemocyanin (KLH) of BALB/c spleen cells that had been primed in vivo with the same antigen. (2) Anti-TNP IgE response induced by the coculture of unprimed C3H B cells with conalbumin (CA)-specific helper T cell clone, D10.G4.1, in the presence of TNP-CA. The former anti-TNP IgE response was not suppressed, and the latter suppressed only partially (less than 30%) by the addition of 100-200 U/ml IFN-gamma. In contrast, polyclonal IgE response in murine B cells that were stimulated by LPS and IL4 was abolished by 10 U/ml IFN-gamma. These results indicate that IgE production from antigen-stimulated B cells, in contrast to those activated polyclonally, are refractory to direct suppression by IFN-gamma.
Ohmori
H
H
Department of Biotechnology, Faculty of Engineering, Okayama University, Japan.
Hikida
M
M
Haruna
K
K
Kishimoto
T
T
D000818:Animals; D000918:Antibody Specificity; D002460:Cell Line; D003207:Conalbumin / Q000276:immunology; D004306:Dose-Response Relationship, Immunologic; D015536:Down-Regulation; D005260:Female; D005786:Gene Expression Regulation / Q000276:immunology*; D006241:Haptens; D006433:Hemocyanins / Q000276:immunology; D007073:Immunoglobulin E / Q000276:immunology*; D007371:Interferon-gamma / Q000276:immunology*; D015847:Interleukin-4 / Q000276:immunology; D008070:Lipopolysaccharides / Q000276:immunology; D008214:Lymphocytes / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H
D006241:Haptens; D008070:Lipopolysaccharides; C054401:trinitrophenyl keyhole limpet hemocyanin; D003207:Conalbumin; D015847:Interleukin-4; D007073:Immunoglobulin E; D007371:Interferon-gamma; D006433:Hemocyanins