High-performance liquid chromatographic assay of the methyl and nucleotide metabolites of 6-mercaptopurine: quantitation of red blood cell 6-thioguanine nucleotide, 6-thioinosinic acid and 6-methylmercaptopurine metabolites in a single sample.
A reversed-phase high-performance liquid chromatographic assay was developed to quantify intracellular metabolites of the cytotoxic drug 6-mercaptopurine in the human red blood cell. The 6-thioguanine nucleotides, 6-thioinosinic acid and 6-methylmercaptopurine metabolites are measured in a single sample. A similar assay measures the parent thiopurine compounds. The limit of quantitation of the assay is 0.03, 0.03 and 0.12 nmol per 8 x 10(8) red blood cells for the 6-thioguanine nucleotides, 6-thioinosinic acid and the 6-methylmercaptopurine metabolites, respectively.
Lennard
L
L
Department of Medicine and Pharmacology, Floor L, Royal Hallamshire Hospital, Sheffield, UK.
Singleton
H J
HJ
D002851:Chromatography, High Pressure Liquid; D004912:Erythrocytes / Q000737:chemistry*; D006801:Humans; D006868:Hydrolysis; D007291:Inosine Monophosphate / Q000031:analogs & derivatives* / Q000097:blood; D015122:Mercaptopurine / Q000097:blood*; D008778:Methylthioinosine / Q000097:blood*; D013873:Thionucleotides / Q000097:blood*
D013873:Thionucleotides; D007291:Inosine Monophosphate; D008778:Methylthioinosine; C003449:thioinosinic acid; D015122:Mercaptopurine