title: 11-hydroperoxyeicosatetraenoic acid is the major dioxygenation product of lipoxygenase isolated from hairy root cultures of Solanum tuberosum.
abstract: The profile of primary dioxygenation products of arachidonic acid catalyzed by lipoxygenase isolated from hairy root cultures of Solanum tuberosum treated with a fungal elicitor was compared to that obtained for the enzyme from potato tubers. 11-Hydroperoxyeicosatetraenoic acid (11-HPETE) was the most abundant dioxygenation product formed followed by 8- and 5-HPETEs in the decreasing order of abundance. In contrast, 5-HPETE is the predominant oxidation product of lipoxygenase from potato tubers. Differences in the defense requirements of storage tuber as compared to roots may be the basis of the differences in regio-specificity demonstrated in this work.
authors: Biotechnology Institute/Chemical Engineering, Pennsylvania State University, University Park 16802.
authors: G R
authors: GR
authors: Reddy
authors: P
authors: P
authors: Reddanna
authors: C C
authors: CC
authors: Reddy
authors: W R
authors: WR
authors: Curtis
mesh_terms: D016718:Arachidonic Acid / Q000378:metabolism; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D008401:Gas Chromatography-Mass Spectrometry; D007700:Kinetics; D015289:Leukotrienes / Q000302:isolation & purification / Q000378:metabolism*; D008084:Lipoxygenase / Q000302:isolation & purification / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism; D011198:Solanum tuberosum / Q000166:cytology / Q000201:enzymology*
chemical_list: D015289:Leukotrienes; C047629:8-hydroperoxyeicosatetraenoic acid; D016718:Arachidonic Acid; C025348:arachidonic acid 5-hydroperoxide; D008084:Lipoxygenase; D010100:Oxygen