title: Kinetics of Lys-bradykinin release by porcine pancreatic kallikrein from rabbit low molecular weight kininogen.
abstract: The initial rates of Lys-bradykinin release by porcine pancreatic kallikrein from rabbit low molecular weight kininogen are found to follow the Michaelis-Menten kinetics with kc = 0.62 sec-1 and Km = 1.93 microM at substrate concentrations 0.3-1.3 microM, but at higher ones the Michaelis dependence is broken. Inhibition of the reaction by its product(s) with Kp < Km is revealed with integral analysis methods in a range of 4.5-270 microM kininogen.
authors: Institute of Biological and Medical Chemistry, Moscow.
authors: L G
authors: LG
authors: Makevnina
authors: G O
authors: GO
authors: Levina
authors: A K
authors: AK
authors: Yatzimirsky
mesh_terms: D000818:Animals; D006868:Hydrolysis; D066298:In Vitro Techniques; D007609:Kallidin / Q000378:metabolism*; D007610:Kallikreins / Q000378:metabolism*; D007700:Kinetics; D007704:Kininogens / Q000378:metabolism*; D010179:Pancreas / Q000201:enzymology; D011817:Rabbits; D013379:Substrate Specificity; D013552:Swine
chemical_list: D007704:Kininogens; D007609:Kallidin; D007610:Kallikreins