title: Noninvasive assessment of metabolism in wounded skin by 31P-NMR in vivo.
abstract: Phosphorus-31 nuclear magnetic resonance techniques using shallow penetrating coils have been used to noninvasively monitor severity and metabolic changes over time in skin wounds in rats. Ratios of phosphocreatine (PCr) to inorganic phosphate (Pi) indicate energy status in both thermal wounds and surgical flaps. In partial and full-thickness scald wounds, reductions in PCr/Pi ratios correlated with burn depth and improved over time postinjury, suggesting wound revascularization. No decrease in intracellular pH was noted in these wounds; the phosphate shifts may be primarily the result of tissue degradation followed by restoration of the microvasculature. Distal regions of caudally based dorsal 3 x 10 cm full-thickness skin flaps reveal progressively lower PCr/Pi ratios to 3-6 hours after elevation as well as drops in pH up to 0.5 units, presumably as a result of anaerobic glycolysis in these tissues. After 24 hours, the intracellular pH returned to normal (7.1-7.2) and the PCr/Pi ratios approached 70%-90% of the well-perfused proximal regions within 3-7 days. These results indicate the establishment of a microvasculature from the underlying bed as the distal regions survive as free grafts. The data demonstrate the potential usefulness of the technique in noninvasive measurement of the biochemical response to injury and wound healing in living organisms.
authors: Department of Medicinal Chemistry, University of Utah, Salt Lake City 84112.
authors: M P
authors: MP
authors: Schweizer
authors: J I
authors: JI
authors: Olsen
authors: J
authors: J
authors: Shelby
authors: J R
authors: JR
authors: Saffle
authors: L R
authors: LR
authors: Chick
authors: M
authors: M
authors: Meyer
authors: J
authors: J
authors: Sylvester
authors: P
authors: P
authors: Jensen
authors: T L
authors: TL
authors: Nagel
mesh_terms: D000818:Animals; D002056:Burns / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D010758:Phosphorus; D051381:Rats; D011917:Rats, Inbred Lew; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000293:injuries / Q000378:metabolism*; D013524:Surgical Flaps
chemical_list: D010758:Phosphorus