Karyotypic analysis of gastric carcinoma cell lines carrying an amplified c-met oncogene.
MKN 45 is a poorly differentiated gastric carcinoma cell line from which the subclone GTL 16 was obtained. Both lines carry an amplification unit derived from chromosome 7 sequences and containing an activated c-met oncogene. Karyotypic analysis showed that GTL 16 derived from a subclone of MKN 45 after endoreduplication. Several clonal abnormalities are evident in both lines; some are frequently observed in gastrointestinal tumors (loss of 17p and monosomy 18). Other consistent anomalies include 6q-, t(8;10) and t(5;8), and inv(16). A marker chromosome (M1), which was previously shown to contain the c-met amplification unit, is constantly duplicated in all GTL 16 metaphases; in contrast, most unidentified markers are retained in only a single copy in GTL 16 cells. These data are in agreement with the hypothesis that the c-met oncogene activation in these gastric cancer cell lines might be related to a gene dosage effect.
Rege-Cambrin
G
G
Department of Biomedical Sciences and Oncology, University of Turin, Italy.
Scaravaglio
P
P
Carozzi
F
F
Giordano
S
S
Ponzetto
C
C
Comoglio
P M
PM
Saglio
G
G
D000230:Adenocarcinoma / Q000235:genetics*; D002869:Chromosome Aberrations; D002872:Chromosome Deletion; D006801:Humans; D007621:Karyotyping; D009006:Monosomy; D009857:Oncogenes; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D019859:Proto-Oncogene Proteins c-met; D013274:Stomach Neoplasms / Q000235:genetics*; D014178:Translocation, Genetic; D014314:Trisomy; D014407:Tumor Cells, Cultured
D011518:Proto-Oncogene Proteins; D011505:Protein-Tyrosine Kinases; D019859:Proto-Oncogene Proteins c-met