Effects of prodigiosin 25-C on cultured cell lines: its similarity to monovalent polyether ionophores and vacuolar type H(+)-ATPase inhibitors.
Prodigiosin 25-C inhibited the proliferation of various cultured cell lines more strongly when concanavalin A (Con A) was added to the cultures. The increase in sensitivity was most evident in T lymphoma YAC-1 cells. The combination of prodigiosin 25-C and Con A induced characteristic morphological changes in these cells. In the presence of Con A, monovalent polyether ionophores and vacuolar type H(+)-ATPase inhibitors induced effects similar to those of prodigiosin 25-C on YAC-1 cells. Prodigiosin 25-C had neither K+ionophore activity nor inhibitory effect on vacuolar type H(+)-ATPase. A Golgi mannosidase II inhibitor, swainsonine, inhibited the proliferation of YAC-1 cells only when Con A was added. Prodigiosin 25-C and swainsonine increased Con A binding receptors on the surface of YAC-1 cells. These results suggest that prodigiosin 25-C affects the intracellular transport and/or processing of glycoproteins.
Kataoka
T
T
Department of Agricultural Chemistry, University of Tokyo, Japan.
Magae
J
J
Kasamo
K
K
Yamanishi
H
H
Endo
A
A
Yamasaki
M
M
Nagai
K
K
D001692:Biological Transport / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D003208:Concanavalin A / Q000494:pharmacology; D006801:Humans; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D007476:Ionophores / Q000494:pharmacology*; D008562:Membrane Glycoproteins / Q000378:metabolism; D011188:Potassium / Q000378:metabolism; D011353:Prodigiosin / Q000031:analogs & derivatives* / Q000494:pharmacology; D006180:Proton-Translocating ATPases / Q000037:antagonists & inhibitors*; D017026:Swainsonine / Q000494:pharmacology; D014407:Tumor Cells, Cultured; D014617:Vacuoles / Q000187:drug effects / Q000201:enzymology
D007166:Immunosuppressive Agents; D007476:Ionophores; D008562:Membrane Glycoproteins; D003208:Concanavalin A; C077331:prodigiosin 25C; D006180:Proton-Translocating ATPases; D011353:Prodigiosin; D017026:Swainsonine; D011188:Potassium