Two intron-containing pre-tRNAs(Tyr) from Triticum aestivum are efficiently processed and spliced in homologous cell-free extract.
Two wheat pre-tRNAs(Tyr) containing introns and flanks are accurately and efficiently spliced in homologous wheat germ S23 extract. The initiation and termination sites upon in vitro transcription in HeLa cell extract have been estimated for both pre-tRNAs.
Szweykowska-Kulińska
Z
Z
Institute of Biopolymer Biochemistry, Adam Mickiewicz University, Poznań, Poland.
D001483:Base Sequence; D002467:Cell Nucleus / Q000502:physiology; D002474:Cell-Free System; D003001:Cloning, Molecular; D017343:Genes, Plant / Q000235:genetics*; D006367:HeLa Cells; D006801:Humans; D007438:Introns / Q000235:genetics*; D008969:Molecular Sequence Data; D012322:RNA Precursors / Q000235:genetics*; D012326:RNA Splicing / Q000235:genetics*; D012365:RNA, Transfer, Tyr / Q000235:genetics*; D014158:Transcription, Genetic / Q000235:genetics; D014908:Triticum / Q000235:genetics*
D012322:RNA Precursors; D012365:RNA, Transfer, Tyr