Polyhydroxy and polyethyleneglycol (meth)acrylate polymers: physical properties and general studies for their use as electrophoresis matrices.
A new series of materials have been tested for their suitability as electrophoresis matrices. The mechanical and optical properties of gels composed of polyethyleneglycol (meth)acrylate esters or polyhydroxy (meth)acrylate esters in water and in various concentrations of organic solvents are described. Several crosslinkers including polyethyleneglycol and polyhydroxy di(meth)acrylates, piperazine diacrylate, and bisacrylamide were used in these studies. Electrophoretic migration and separation of a series of protein standards through polyethyleneglycol methacrylate (PEGM) 200, PEGM 400, and glyceryl methacrylate is demonstrated. Further, copolymerization of all of the monomers with acrylamide was performed and the distribution of monomer incorporation into the polymer network calculated. All monomers and copolymers that were examined by IR spectroscopy showed greater than 99% polymerization. These results justify their further study for biomolecule separations.
Zewert
T
T
NSF Center for Molecular Biotechnology, Beckman Institute, Pasadena, CA 91125.
Harrington
M
M
D003432:Cross-Linking Reagents; D004586:Electrophoresis / Q000295:instrumentation*; D015394:Molecular Structure; D011092:Polyethylene Glycols / Q000737:chemistry*; D011102:Polyhydroxyethyl Methacrylate / Q000737:chemistry*; D011109:Polymethacrylic Acids / Q000737:chemistry*; D011506:Proteins / Q000032:analysis; D012997:Solvents; D013053:Spectrophotometry; D013055:Spectrophotometry, Infrared; D014783:Viscosity; D014867:Water
D003432:Cross-Linking Reagents; D011109:Polymethacrylic Acids; D011506:Proteins; D012997:Solvents; D014867:Water; D011102:Polyhydroxyethyl Methacrylate; D011092:Polyethylene Glycols