title: Detection of DNA damage in stimulated human lymphocytes after enflurane exposure in vitro.
abstract: DNA damage was detected by nucleoid sedimentation in human lymphocytes stimulated with pokeweed mitogen after exposure to enflurane. Enflurane induces DNA damage at an exposure concentration of 0.2 vol%. Higher enflurane concentrations increase the rate of DNA damage. The DNA damage seen after exposure to enflurane concentrations of 0.2 and 3.0% vol is comparable to damage after X-radiation of 0.1 and 0.7 Gy. DNA single-strand breaks can be demonstrated by nucleoid sedimentation and can indicate damage before DNA repair begins. Therefore, detected DNA single-strand breaks may be reversible. However, DNA repair is not always successful and an increased number of DNA single-strand breaks could lead to irreversible DNA damage. The method of nucleoid sedimentation helps to show DNA damage in proliferating cells after exposure to volatile anesthetics or therapeutic gases.
authors: Department of Physiological Chemistry, Duesbergweg, Federal Republic of Germany.
authors: M
authors: M
authors: Reitz
authors: K
authors: K
authors: DasGupta
authors: L
authors: L
authors: Brandt
mesh_terms: D002478:Cells, Cultured; D004249:DNA Damage; D004277:DNA, Single-Stranded / Q000187:drug effects*; D004737:Enflurane / Q000633:toxicity*; D006801:Humans; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000187:drug effects*
chemical_list: D004277:DNA, Single-Stranded; D004737:Enflurane