Electrostatic interaction influences cell adhesion?
The effect of electrostatic forces on the adhesion of LEP-19 diploid embryonal fibroblasts, Hep-2 laryngeal carcinoma cells, Raji lymphoblastoma cells and Sp 2/0 myeloma cells was examined in vitro. Adhesivity of all tested cell lines was higher on the cationized glass than on untreated or anionized glass. The negatively charged sialic acids on the cell surface play a role in cell adhesion. The participation of electrostatic interaction is independent of the energy metabolism in serum-free conditions.
Smetana
K
K
Department of Anatomy, Charles University, First Faculty of Medicine, Prague, Czech and Slovak Federal Republic.
Vytásek
R
R
Stol
M
M
D000838:Anions; D002412:Cations; D002448:Cell Adhesion; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D016895:Culture Media, Serum-Free; D005347:Fibroblasts / Q000502:physiology*; D005898:Glass; D006801:Humans; D008863:Microspheres; D014411:Neoplastic Stem Cells / Q000502:physiology*; D009439:Neuraminidase / Q000494:pharmacology; D012794:Sialic Acids / Q000502:physiology; D013499:Surface Properties; D013696:Temperature; D014407:Tumor Cells, Cultured
D000838:Anions; D002412:Cations; D016895:Culture Media, Serum-Free; D012794:Sialic Acids; D009439:Neuraminidase