Purification and characterization of a carbonic anhydrase II inhibitor from porcine plasma.
Plasma from many vertebrates, including pigs, contains a soluble component that inhibits the CO2 hydrase activity of carbonic anhydrase (CA). This activity was purified to homogeneity (approximately 4000-fold) from porcine plasma using a combination of DEAE-Affi-Gel Blue chromatography and carbonic anhydrase II-affinity chromatography, yielding 16 mg of inhibitory protein/L of plasma. This protein, porcine inhibitor of carbonic anhydrase (pICA), is a monomeric protein with an apparent molecular mass of 79 kDa, as determined by electrospray mass spectrometry. As isolated, pICA contains about 3 kDa of N-linked glycosylation removable by peptide N-glycosidase F. pICA inhibits CA reversibly with a 1:1 stoichiometry. pICA is a potent and specific inhibitor of the CA II isozyme, with Ki < 0.1 nM for porcine CA II at pH 7.4. Although the Ki is dependent on the CA isozyme type (CA II << CA IV << CA III approximately CA I), it is relatively insensitive to the species source, as long as it is mammalian. The Ki is pH dependent with log Ki decreasing linearly as the pH decreases, implicating at least one ionizable group with the pKa < or = 6.5 in the binding interaction. The isozyme and species dependence of the inhibition suggest that pICA interacts with amino acids on the surface of CA II.
Roush
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ED
Department of Biochemistry, Duke University Medical Center, Durham, North Carolina 27710.
Fierke
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CA
D000818:Animals; D002257:Carbonic Anhydrase Inhibitors / Q000097:blood / Q000737:chemistry / Q000302:isolation & purification*; D002256:Carbonic Anhydrases / Q000187:drug effects; D004591:Electrophoresis, Polyacrylamide Gel; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D011487:Protein Conformation; D013045:Species Specificity; D013379:Substrate Specificity; D013552:Swine
D002257:Carbonic Anhydrase Inhibitors; D002256:Carbonic Anhydrases