Purification of biologically active human plasma transthyretin by dye-affinity chromatography: studies on dye leakage and possibility of heat treatment for virus inactivation.
The application of a purification procedure for the industrial preparation from human plasma of a therapeutic protein may be hindered by several safety concerns. The dye leaching from Remazol Yellow GGL-Sepharose used for the affinity chromatography of human plasma transthyretin was quantitatively studied by a sensitive competitive enzyme immunoassay. The possibility of including a heat treatment step for virus inactivation in the purification process while preserving the biochemical and functional characteristics of the protein is also reported.
Regnault
V
V
INSERM U 284, Faculté de Médecine, Vandoeuvre-lès-Nancy France.
Rivat
C
C
Vallar
L
L
Geschier
C
C
Stolz
J F
JF
D001391:Azo Compounds; D002846:Chromatography, Affinity / Q000379:methods*; D004396:Coloring Agents; D004340:Drug Contamination; D004356:Drug Storage; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006358:Hot Temperature; D006801:Humans; D007457:Iodine Radioisotopes; D011228:Prealbumin / Q000302:isolation & purification*; D013974:Thyroxine / Q000097:blood; D018406:Virus Physiological Phenomena
D001391:Azo Compounds; D004396:Coloring Agents; D007457:Iodine Radioisotopes; D011228:Prealbumin; C035187:reactive yellow 13; D013974:Thyroxine