A new high sensitivity 19F probe for labeling cysteine groups of proteins.
A novel iodoacetamide label 4-perfluoro-tert-butyl-phenyliodoacetamide (PFP) containing nine fluorine atoms in equivalent positions has been synthesized. It provides a homogeneous 19F NMR resonance line which can be detected with high sensitivity when coupled to proteins. As an example, the sulfhydryl groups of actin have been labeled with PFP; < 100 nmol of this medium sized protein (corresponding to 2.5 mL of a 40 microM solution) can be detected easily in a single scan at 470 MHz.
Kalbitzer
H R
HR
Max-Planck-Institute for Medical Research, Heidelberg, Germany.
Rohr
G
G
Nowak
E
E
Goody
R S
RS
Kuhn
W
W
Zimmermann
H
H
D000081:Acetamides / Q000138:chemical synthesis*; D000199:Actins / Q000032:analysis; D000818:Animals; D003545:Cysteine / Q000032:analysis*; D005461:Fluorine / Q000032:analysis*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D011506:Proteins / Q000032:analysis*; D011817:Rabbits; D012680:Sensitivity and Specificity; D013057:Spectrum Analysis; D013438:Sulfhydryl Compounds / Q000032:analysis; D005466:Fluorocarbons
D000081:Acetamides; D000199:Actins; D003545:Cysteine; D005461:Fluorine; D011506:Proteins; D013438:Sulfhydryl Compounds; C079493:4-perfluoro-tert-butyl-phenyliodoacetamide; D005466:Fluorocarbons