Influence of high-level gentamicin resistance and beta-hemolysis on susceptibility of enterococci to the bactericidal activities of ampicillin and vancomycin.
The bactericidal activities of ampicillin and vancomycin against 40 recent isolates of Enterococcus faecalis were examined by kill-kinetic studies at concentrations of 4 x the MIC and 20 micrograms/ml. Greater killing was seen with ampicillin (3.57 +/- 0.87 and 2.50 +/- 1.09 log10 CFU/ml, respectively; mean +/- standard deviation) than with vancomycin (1.23 +/- 0.65 and 1.05 +/- 0.57 log10 CFU/ml, respectively). Highly gentamicin-resistant strains showed a tendency toward reduced susceptibility to killing; beta-hemolytic strains were more susceptible than nonhemolytic strains when exposed to ampicillin at 20 micrograms/ml. Within each group, individual isolates demonstrated great variability in susceptibility to killing by the drugs.
Cercenado
E
E
Department of Medicine, New England Deaconess Hospital, Boston, Massachusetts 02115.
Eliopoulos
G M
GM
Wennersten
C B
CB
Moellering
R C
RC
D000667:Ampicillin / Q000494:pharmacology*; D004352:Drug Resistance, Microbial / Q000502:physiology; D013293:Enterococcus faecalis / Q000187:drug effects*; D005839:Gentamicins / Q000494:pharmacology*; D006461:Hemolysis / Q000187:drug effects*; D008826:Microbial Sensitivity Tests; D015203:Reproducibility of Results; D014640:Vancomycin / Q000494:pharmacology*
D005839:Gentamicins; D014640:Vancomycin; D000667:Ampicillin