A cell line that can induce thymocyte positive selection.
The thymus positively selects thymocytes that bear T-cell receptors which recognize antigen presented by self major histocompatibility complex (MHC) proteins. Positive selection is usually driven by MHC products on radiation-resistant cortical epithelial cells. It is unknown whether positive selection is mediated by all thymic epithelial cells or by some specialized subsets. Here we introduce an H-2b-expressing thymic epithelial cell line into the thymuses of lethally irradiated H-2k animals reconstituted with H-2b/k F1 BM or fetal liver cells. I-Ab-restricted T cells are found in these animals, demonstrating that selection occurs on the introduced epithelial cells.
Hugo
P
P
Howard Hughes Medical Institute, National Jewish Center for Immunology and Respiratory Medicine, Denver, Colorado 80206.
Kappler
J W
JW
Godfrey
D I
DI
Marrack
P C
PC
D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology*; D016026:Bone Marrow Transplantation / Q000276:immunology; D002460:Cell Line; D002478:Cells, Cultured; D002999:Clone Cells; D004768:Enterotoxins / Q000276:immunology; D004848:Epithelium / Q000276:immunology; D005260:Female; D016332:Fetal Tissue Transplantation / Q000276:immunology; D006183:H-2 Antigens / Q000276:immunology; D006825:Hybridomas / Q000276:immunology; D007371:Interferon-gamma / Q000494:pharmacology; D016031:Liver Transplantation / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D010047:Ovalbumin / Q000276:immunology; D013154:Spleen / Q000276:immunology; D013211:Staphylococcus aureus; D013601:T-Lymphocytes / Q000276:immunology*; D013950:Thymus Gland / Q000276:immunology* / Q000528:radiation effects
D004768:Enterotoxins; D006183:H-2 Antigens; C031818:enterotoxin B, staphylococcal; D007371:Interferon-gamma; D010047:Ovalbumin