Detection of Mycobacterium leprae and the potential for monitoring antileprosy drug therapy directly from skin biopsies by PCR.
An improved protocol for PCR analysis of Mycobacterium leprae-infected tissues, based on enzymatic lysis, has been developed and used to demonstrate the feasibility of using PCR for detecting M. leprae in routine skin biopsies taken from leprosy patients throughout the clinical spectrum. Of 92 multibacillary patients tested, 99% were PCR-positive using gel electrophoresis or DNA hybridization to detect the amplified product. Similar analysis of paucibacillary patients, in which only one of 27 biopsies had demonstrable AFB microscopically, gave a positivity rate of 74%. No PCR signals were demonstrated from skin biopsies from seven patients with non-leprosy dermatoses and one AIDS patient with a disseminated atypical mycobacteriosis. Evaluation of leprosy patients with antileprosy drug therapy prior to biopsy demonstrated that PCR signals were either greatly diminished or absent after 2 months of continuous antibiotic therapy. PCR was also able to detect the presence of M. leprae in tissues of patients receiving antibacterial therapy when patients were suspected of harbouring drug-resistant M. leprae.
Williams
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DL
G. W. Long Hansen's Disease Center, LSU SVM, Baton Rouge 70894.
Gillis
T P
TP
Fiallo
P
P
Job
C K
CK
Gelber
R H
RH
Hill
C
C
Izumi
S
S
D000900:Anti-Bacterial Agents; D001706:Biopsy; D002991:Clofazimine / Q000627:therapeutic use*; D015342:DNA Probes; D004269:DNA, Bacterial / Q000235:genetics; D003622:Dapsone / Q000627:therapeutic use*; D004359:Drug Therapy, Combination / Q000627:therapeutic use; D004587:Electrophoresis, Agar Gel; D005240:Feasibility Studies; D006801:Humans; D007918:Leprosy / Q000188:drug therapy / Q000382:microbiology*; D009166:Mycobacterium leprae / Q000235:genetics / Q000302:isolation & purification*; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D012293:Rifampin / Q000627:therapeutic use*; D012680:Sensitivity and Specificity; D012867:Skin / Q000382:microbiology* / Q000473:pathology
D000900:Anti-Bacterial Agents; D015342:DNA Probes; D004269:DNA, Bacterial; D003622:Dapsone; D002991:Clofazimine; D012293:Rifampin