title: Moderate heat treatment of bone allografts. Experimental results of osteointegration.
abstract: The use of bone allografts is often essential in orthopedic surgery. Strict donor screening, including HIV testing 3 months postoperatively, is mandatory before a transplant may be used. Yet these measures do not definitely rule out the possibility of HIV transmission, as there is a window period before infection is revealed by blood testing. Accordingly, there is a need for virus inactivation methods that can be used on bone allografts. As radiation treatment and chemical methods have a number of disadvantages, we chose a moderate heat treatment of 65 degrees C for a series of animal experiments. In 12 rabbit femoral condyles, moderate-heat-treated bone allografts were implanted into 6-mm drill holes. Twelve normal allografts and 12 empty drill holes served as controls. Radiologic and histological evaluation up to 12 weeks postoperatively revealed slow spontaneous bone remodeling from the rim to the center of the empty cavities. Normal deep frozen allografts were quickly intergrated after a short period of osteoclast reaction around the transplant, with occasional bone bridges between host and allograft. The examination of heat-treated allografts showed no differences to the controls, including morphologic aspects and the time course of osteointegration. Five zones of bone repair and osteointegration were distinguished. We conclude that thermal treatment of bone allografts has adverse effects on osteointegration in the rabbit femoral condyle. Thus, it may contribute to improving safety in human bone transplantation.
authors: Orthopedic Department, Ludwig Maximilians University, Munich, Federal Republic of Germany.
authors: J H
authors: JH
authors: Kühne
authors: R
authors: R
authors: Bartl
authors: C
authors: C
authors: Hammer
authors: H J
authors: HJ
authors: Refior
authors: V
authors: V
authors: Jansson
authors: M
authors: M
authors: Zimmer
mesh_terms: D000818:Animals; D016025:Bone Transplantation / Q000379:methods*; D005347:Fibroblasts / Q000473:pathology; D015658:HIV Infections / Q000517:prevention & control; D006358:Hot Temperature / Q000627:therapeutic use*; D016348:Osseointegration; D010006:Osteoblasts / Q000473:pathology; D010010:Osteoclasts / Q000473:pathology; D011817:Rabbits; D014184:Transplantation, Homologous