Immunocytochemical and in situ hybridization detection of hypothalamic neuropeptides from postmortem unfixed rat brains.
The effects of postmortem delay on neuropeptide-containing perikarya was studied in the paraventricular nucleus (PVN) of the rat hypothalamus. Serial sections from brains kept in the skull after death for 6 h and immunocytochemically processed for oxytocin (OT), vasopressin (AVP) and corticotropin releasing factor (CRF) or hybridized in situ for CRF resulted in the well preserved phenotypic expression and stability of mRNA of the aforementioned neuropeptides. Furthermore in most cases, AVP and CRF expression was discernibly enhanced relative to prefixed immunopositive tissue. Results of this study suggest that postmortem variables do not significantly alter the neurochemical coding of magnocellular or parvocellular neurosecretory systems, and support the view that rat and human brain topography can be investigated from tissue left in situ after death for a relatively long period of time.
Torres
G
G
Clayton Foundation Laboratories for Peptide Biology, Salk Institute, La Jolla, CA 92037.
Lee
S
S
Rivier
C
C
D000315:Adrenalectomy; D000818:Animals; D001127:Arginine Vasopressin / Q000096:biosynthesis / Q000276:immunology; D003346:Corticotropin-Releasing Hormone / Q000096:biosynthesis / Q000276:immunology; D007031:Hypothalamus / Q000276:immunology / Q000378:metabolism*; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008297:Male; D009479:Neuropeptides / Q000276:immunology / Q000378:metabolism*; D010121:Oxytocin / Q000096:biosynthesis / Q000276:immunology; D011180:Postmortem Changes; D012333:RNA, Messenger / Q000096:biosynthesis / Q000276:immunology; D051381:Rats; D017207:Rats, Sprague-Dawley; D016707:Tissue Fixation
D009479:Neuropeptides; D012333:RNA, Messenger; D001127:Arginine Vasopressin; D010121:Oxytocin; D003346:Corticotropin-Releasing Hormone