title: Inhibition of norepinephrine secretion from digitonin permeabilized PC12 cells by botulinum type D toxin.
abstract: Botulinum type D neurotoxin inhibited Ca(2+)-evoked norepinephrine secretion in digitonin permeabilized PC12 cells. Inhibition by the toxin required prior incubation with dithiothreitol (DTT). The inhibition was dependent on both concentration and incubation times of the toxin, and was affected by Ca2+ concentration. With less than 0.7 microM Ca2+ almost complete inhibition was observed; however, above 0.7 microM, Ca2+ stimulated additional norepinephrine release in a dose-dependent manner.
authors: Department of Microbiology, Sapporo Medical College, Japan.
authors: N
authors: N
authors: Yokosawa
authors: N
authors: N
authors: Fujii
authors: K
authors: K
authors: Tsuzuki
authors: K
authors: K
authors: Kimura
authors: B
authors: B
authors: Syuto
authors: K
authors: K
authors: Moriishi
authors: K
authors: K
authors: Oguma
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D001905:Botulinum Toxins / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism / Q000502:physiology; D004072:Digitonin / Q000494:pharmacology*; D004229:Dithiothreitol / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D009638:Norepinephrine / Q000378:metabolism*; D016716:PC12 Cells; D010703:Phorbol Esters / Q000494:pharmacology; D051381:Rats
chemical_list: D000911:Antibodies, Monoclonal; D010703:Phorbol Esters; D001905:Botulinum Toxins; D004072:Digitonin; D002118:Calcium; D004229:Dithiothreitol; D009638:Norepinephrine