Inhibition of norepinephrine secretion from digitonin permeabilized PC12 cells by botulinum type D toxin.
Botulinum type D neurotoxin inhibited Ca(2+)-evoked norepinephrine secretion in digitonin permeabilized PC12 cells. Inhibition by the toxin required prior incubation with dithiothreitol (DTT). The inhibition was dependent on both concentration and incubation times of the toxin, and was affected by Ca2+ concentration. With less than 0.7 microM Ca2+ almost complete inhibition was observed; however, above 0.7 microM, Ca2+ stimulated additional norepinephrine release in a dose-dependent manner.
Yokosawa
N
N
Department of Microbiology, Sapporo Medical College, Japan.
Fujii
N
N
Tsuzuki
K
K
Kimura
K
K
Syuto
B
B
Moriishi
K
K
Oguma
K
K
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D001905:Botulinum Toxins / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism / Q000502:physiology; D004072:Digitonin / Q000494:pharmacology*; D004229:Dithiothreitol / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D009638:Norepinephrine / Q000378:metabolism*; D016716:PC12 Cells; D010703:Phorbol Esters / Q000494:pharmacology; D051381:Rats
D000911:Antibodies, Monoclonal; D010703:Phorbol Esters; D001905:Botulinum Toxins; D004072:Digitonin; D002118:Calcium; D004229:Dithiothreitol; D009638:Norepinephrine