[Anti-Rho[D] antigen monoclonal antibodies. II. Serological characteristics of monoclonal IgG1 antibodies].
Four IgG1 monoclonal antibodies (MoAbs) to the Rh antigen D, produced by stable Epstein-Barr virus transformed B-lymphoblastoid cell lines were assessed in different serological tests. Their suitability in blood group typing was shown in antiglobulin, enzyme or albumin methods. The specificity and activity of MoAbs was tested with a panel of red cells of various Rh-phenotypes. The supernatants of all four lines showed anti-D specificity and ability to react with Du red cells. Mean level of MoAbs concentration was near 10 micrograms/ml. These human anti-D MoAbs proved to be useful D-typing diagnostic reagents.
Olovnikova
N I
NI
Belkina
E V
EV
Lemeneva
L N
LN
Berkovskiĭ
A L
AL
Deriugina
E I
EI
Chertkov
I L
IL
D000372:Agglutination Tests / Q000379:methods; D000911:Antibodies, Monoclonal / Q000276:immunology*; D001788:Blood Grouping and Crossmatching / Q000379:methods; D002478:Cells, Cultured; D003298:Coombs Test / Q000379:methods; D003470:Culture Media; D004912:Erythrocytes / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology*; D066298:In Vitro Techniques; D007518:Isoantibodies / Q000276:immunology*; D012203:Rh Isoimmunization / Q000175:diagnosis* / Q000276:immunology; D012204:Rh-Hr Blood-Group System / Q000276:immunology*
D000911:Antibodies, Monoclonal; D003470:Culture Media; D007074:Immunoglobulin G; D007518:Isoantibodies; D012204:Rh-Hr Blood-Group System
Monoklonal'nye antitela k Rho[D]-antigenu sistemy rezus. II. Serologicheskaia kharakteristika monoklonal'nykh IgG1-antitel.