A red cell band 3 variant with altered stilbene disulphonate binding is associated with the Diego (Dia) blood group antigen.
1. We have shown that the Dia antigen of the Diego blood group system is associated with the presence of red cell band 3 Memphis, but not all band 3 Memphis samples carry the Dia antigen. 2. The band 3 Memphis associated with the Dia antigen was covalently labelled by 4,4'-di-isothiocyanato-1,2-diphenylethane-2,2'-disulphonic acid (H2DIDS) more readily than was normal band 3 or band 3 Memphis not associated with the Dia antigen. This altered reactivity with H2DIDS has previously been noted for a band 3 Memphis sub-type designated variant 2. 3. This is the first example of a band 3 polymorphism associated with an antigenic change in the extracellular region of the band 3 polypeptide and with altered H2DIDS binding.
Spring
F A
FA
Department of Immunochemistry, South Western Regional Transfusion Centre, Bristol, U.K.
Bruce
L J
LJ
Anstee
D J
DJ
Tanner
M J
MJ
D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid / Q000031:analogs & derivatives* / Q000378:metabolism; D001457:Anion Exchange Protein 1, Erythrocyte / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D001789:Blood Group Antigens / Q000502:physiology*; D004912:Erythrocytes / Q000276:immunology / Q000378:metabolism; D005110:Extracellular Space / Q000378:metabolism; D014644:Genetic Variation / Q000502:physiology; D006801:Humans; D011110:Polymorphism, Genetic; D011485:Protein Binding
D001457:Anion Exchange Protein 1, Erythrocyte; D001789:Blood Group Antigens; C070316:band 3 protein Memphis; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid; D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid