title: Evidence for the presence and location of annexins in human platelets.
abstract: In this study the identity of annexins in human platelets has been determined together with their ability to be released by agents which induce platelet degranulation. The presence of proteins cross-reacting to antibodies against annexins I and V was detected in human platelets. However, the study provided evidence that these annexins are not located on the surface of the plasma membrane in a Ca++ dependent manner. Moreover, activation of platelets with several agents which induced platelet degranulation did not cause release of annexins I or V as determined by both immunoblotting and ELISA.
authors: Department of Pharmacology, University of Bath, Avon, UK.
authors: C T
authors: CT
authors: Murphy
authors: S H
authors: SH
authors: Peers
authors: R A
authors: RA
authors: Forder
authors: R J
authors: RJ
authors: Flower
authors: F
authors: F
authors: Carey
authors: J
authors: J
authors: Westwick
mesh_terms: D017305:Annexin A1 / Q000097:blood; D017306:Annexin A2 / Q000097:blood; D017314:Annexin A4 / Q000097:blood; D017304:Annexin A5 / Q000097:blood; D017302:Annexins / Q000097:blood*; D000906:Antibodies; D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism*; D015153:Blotting, Western; D003429:Cross Reactions; D003594:Cytoplasmic Granules / Q000378:metabolism; D004492:Edetic Acid / Q000494:pharmacology; D004533:Egtazic Acid / Q000494:pharmacology; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D015759:Ionomycin / Q000494:pharmacology; D007700:Kinetics; D010972:Platelet Activating Factor / Q000494:pharmacology; D015539:Platelet Activation; D012701:Serotonin / Q000097:blood; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D013917:Thrombin / Q000494:pharmacology
chemical_list: D017305:Annexin A1; D017306:Annexin A2; D017314:Annexin A4; D017304:Annexin A5; D017302:Annexins; D000906:Antibodies; D010972:Platelet Activating Factor; D012701:Serotonin; D004533:Egtazic Acid; D015759:Ionomycin; D004492:Edetic Acid; D013917:Thrombin; D013755:Tetradecanoylphorbol Acetate