title: An improved method for quantitative culture of Malassezia furfur.
abstract: Quantitative culture of Malassezia furfur from clinically healthy skin in 25 individuals was performed with two different methods using contact plates. The best results were obtained when a glucose peptone yeast extract medium, with the addition of milk, Tween-60, glycerol and glycerol monostearate was used. Different techniques for incubation and the reproducibility of this method were evaluated. Incubation can be done in a plastic bag at 32 or 37 degrees C. This new method is simple, the colonies are easy to identify and the counts are high and reliable.
authors: Department of Dermatology, University of Gothenburg, Sahlgrenska Hospital, Sweden.
authors: I M
authors: IM
authors: Bergbrant
authors: A
authors: A
authors: Igerud
authors: P
authors: P
authors: Nordin
mesh_terms: D003470:Culture Media; D066298:In Vitro Techniques; D008290:Malassezia / Q000254:growth & development* / Q000302:isolation & purification; D012867:Skin / Q000382:microbiology*
chemical_list: D003470:Culture Media