Comparative effect of fasting on acetoacetate and D-3-hydroxybutyrate metabolism in the newborn chick.
The effect of 24 h fasting on ketone body utilization by three extranervous tissues, liver, duodenum and kidney, was studied in two critical ages of neonatal chick: 4 and 9 days. In 4-day-old chick, plasma concentration of 3-hydroxybutyrate increased about 9-fold after 24 h starvation, while in 9-day-old chick this parameter increased about 23-fold in the same conditions. Hepatic lipogenesis from both precursors sharply decreased by fasting. Changes in the lipogenic activity of duodenum were less patent. However, we have found a clear increase in lipogenesis in chick kidney after 24 h starvation. CO2 production from acetoacetate was higher than that found from hydroxybutyrate. No significant differences in the acetoacetate oxidation to CO2 was observed in any tissue assayed after 24 h fasting. 14C incorporation from ketone bodies into amino acids was clearly decreased in kidney from 9-day-old chick by fasting. In liver and duodenum, acetoacetate incorporation into amino acids was higher than that from hydroxybutyrate.
Linares
A
A
Departamento de Bioquímica y Biología Molecular, Universidad de Granada, Spain.
Diaz
R
R
Caamaño
G J
GJ
Gonzalez
F J
FJ
Garcia-Peregrin
E
E
D020155:3-Hydroxybutyric Acid; D000090:Acetoacetates / Q000378:metabolism*; D000596:Amino Acids / Q000096:biosynthesis; D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism; D002245:Carbon Dioxide / Q000378:metabolism; D002645:Chickens; D004386:Duodenum / Q000254:growth & development / Q000378:metabolism; D005215:Fasting / Q000502:physiology*; D006885:Hydroxybutyrates / Q000378:metabolism*; D007668:Kidney / Q000254:growth & development / Q000378:metabolism; D008055:Lipids / Q000096:biosynthesis; D008099:Liver / Q000254:growth & development / Q000378:metabolism; D010084:Oxidation-Reduction
D000090:Acetoacetates; D000596:Amino Acids; D006885:Hydroxybutyrates; D008055:Lipids; D002245:Carbon Dioxide; C016635:acetoacetic acid; D020155:3-Hydroxybutyric Acid