title: Reliability of quantal parameter estimates and their changes during long-term potentiation in guinea pig hippocampal slices.
abstract: Previously we found increases in quantal content (m) and smaller increases in quantal size (v) during long-term potentiation (LTP) in CA1 of hippocampal slices. However, the validity of the deconvolution technique was questioned recently because v estimates correlated with the noise standard deviation (Sn). In computer simulations we show a double-step dependence of v on Sn/v: correct v estimates (within +/- 20%) for Sn/v < or = 0.5 and overestimates (correlated with Sn) for Sn/v > 0.5. A novel 'noise addition' procedure is proposed for accepting reliable solutions on the basis of the double-step relationship between v and Sn. Quantal analysis of LTP for more reliable solutions confirmed previous conclusions.
authors: Brain Institute, Russian Academy of Medical Sciences, Moscow.
authors: L L
authors: LL
authors: Voronin
authors: U
authors: U
authors: Kuhnt
authors: N V
authors: NV
authors: Ivanov
authors: A G
authors: AG
authors: Gusev
mesh_terms: D000818:Animals; D004558:Electric Stimulation; D005073:Evoked Potentials, Somatosensory / Q000187:drug effects; D006168:Guinea Pigs; D006624:Hippocampus / Q000187:drug effects* / Q000502:physiology; D066298:In Vitro Techniques; D008959:Models, Neurological; D009010:Monte Carlo Method; D009473:Neuronal Plasticity / Q000502:physiology*