A high angle neutron fibre diffraction study of the hydration of the A conformation of the DNA double helix.
A high angle neutron fibre diffraction study of the distribution of water around the A-form of DNA has been performed using the diffractometer D19 at the Institut Laue-Langevin, Grenoble. These experiments have exploited the ability to replace H2O surrounding the DNA by D2O so that isotopic difference Fourier maps can be computed in which peaks are identified with the distribution of water in the unit cell. All peaks of significant height have been accounted for by four families of water molecules whose positions and occupancies have been determined using least squares refinement. The coordinates of the water peaks making up each family do not deviate significantly from a regular helical arrangement with the same parameters as the DNA. Two of these families are of particular interest. The first consists of water molecules in the major groove linking successive charged phosphate oxygens along the polynucleotide chains. The second is associated with bases in the major groove and forms a central core of density along the helix axis. These two families provide a layer of hydration lining the interior wall of the major groove leaving a central channel to accommodate cations. The relationship between these observations and conformational stability is discussed.
Langan
P
P
Department of Physics, Keele University, Staffordshire.
Forsyth
V T
VT
Mahendrasingam
A
A
Pigram
W J
WJ
Mason
S A
SA
Fuller
W
W
D004247:DNA / Q000737:chemistry* / Q000648:ultrastructure; D005583:Fourier Analysis; D009502:Neutrons; D009690:Nucleic Acid Conformation; D014867:Water / Q000737:chemistry
D014867:Water; D004247:DNA