title: Chemiluminescence of polymorphonuclear granulocytes in the presence of selected Candida species.
abstract: We monitored the respiratory burst of polymorphonuclear granulocytes (PMN) during infection in vitro with 25 strains of six Candida species with different extracellular proteolytic activity by chemiluminescence (CL). The CL-response of PMNs to viable Candida blastoconidia was compared to the response to ethanol-fixed cells of the same strain. Ethanol-fixed blastoconidia of all tested fungal strains uniformly increased the CL-response on contact. Viable fungal blastoconidia induced different CL-responses: (1) virulent C. albicans and C. tropicalis induced a low CL-response, (2) less virulent C. glabrata, C. parapsilosis and C. krusei induced CL-responses which reflected strain-specific differences, and (3) viable blastoconidia of attenuated C. guilliermondii uniformly caused a high PMN CL-response. After the CL-measurement, the killing of Candida by PMNs was assayed. High CL-response could be correlated to an augmented killing of the yeasts. These results were discussed with respect to a factor of fungal virulence, the secretion of acid proteinase.
authors: Department of Medical Microbiology, University of Göttingen, Germany.
authors: M
authors: M
authors: Borg-von Zepelin
authors: P
authors: P
authors: Schuff-Werner
mesh_terms: D002175:Candida / Q000276:immunology*; D005609:Free Radicals / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D008163:Luminescent Measurements; D009504:Neutrophils / Q000276:immunology / Q000378:metabolism*; D016897:Respiratory Burst / Q000502:physiology*
chemical_list: D005609:Free Radicals