Oligonucleotide sequences required for natural killer cell activation.
Based on the previous finding that certain 30-mer single-stranded oligodeoxyribonucleotides (oligonucleotides) having particular 6-mer palindromic sequences could induce interferon-alpha and -gamma, and enhance natural killer activity, the present study was carried out to clarify the entire relationship between the activity and the sequence of 30-mer oligonucleotides. The results indicated that the activity depended critically on the presence of particular palindromic sequences including the 5'-CG-3' motif(s). The size and the number of palindromes as well as the extra-palindromic sequences also influenced the activity. An oligonucleotide with a 10-mer palindrome and extra-palindromic oligoguanylate sequences showed the strongest activity among the oligonucleotides tested.
Kuramoto
E
E
Mitsui Pharmaceuticals, Inc., Institute of Biological Science, Chiba.
Yano
O
O
Kimura
Y
Y
Baba
M
M
Makino
T
T
Yamamoto
S
S
Yamamoto
T
T
Kataoka
T
T
Tokunaga
T
T
D000818:Animals; D001483:Base Sequence; D007694:Killer Cells, Natural / Q000187:drug effects / Q000502:physiology*; D008213:Lymphocyte Activation / Q000187:drug effects* / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000494:pharmacology*; D013268:Stimulation, Chemical; D013329:Structure-Activity Relationship
D009841:Oligonucleotides