title: Site-directed mutagenesis and X-ray crystallography of two phospholipase A2 mutants: Y52F and Y73F.
abstract: Tyr52 and Tyr73 are conserved amino acid residues throughout all vertebrate phospholipases A2. They are part of an extended hydrogen bonding system that links the N-terminal alpha-NH3(+)-group to the catalytic residues His48 and Asp99. These tyrosines were replaced by phenylalanines in a porcine pancreatic phospholipase A2 mutant, in which residues 62-66 had been deleted (delta 62-66PLA2). The mutations did not affect the catalytic properties of the enzyme, nor the folding kinetics. The stability against denaturation by guanidine hydrochloride was decreased, however. To analyse how the enzyme compensates for the loss of the tyrosine hydroxyl group, the X-ray structures of the delta Y52F and delta Y73F mutants were determined. After crystallographic refinement the final crystallographic R-factors were 18.1% for the delta Y52F mutant (data between 7 and 2.3 A resolution) and 19.1% for the delta Y73F mutant (data between 7 and 2.4 A resolution). No conformational changes occurred in the mutants compared with the delta 62-66PLA2, but an empty cavity formed at the site of the hydroxyl group of the former tyrosine. In both mutants the Asp99 side chain loses one of its hydrogen bonds and this might explain the observed destabilization.
authors: Laboratory of Biophysical Chemistry, University of Groningen, The Netherlands.
authors: M M
authors: MM
authors: Thunnissen
authors: P A
authors: PA
authors: Franken
authors: G H
authors: GH
authors: de Haas
authors: J
authors: J
authors: Drenth
authors: K H
authors: KH
authors: Kalk
authors: H M
authors: HM
authors: Verheij
authors: B W
authors: BW
authors: Dijkstra
mesh_terms: D004795:Enzyme Stability; D006860:Hydrogen Bonding; D008958:Models, Molecular; D016297:Mutagenesis, Site-Directed; D010741:Phospholipases A / Q000737:chemistry* / Q000235:genetics; D054467:Phospholipases A2; D011487:Protein Conformation; D011489:Protein Denaturation; D017510:Protein Folding; D013329:Structure-Activity Relationship; D014961:X-Ray Diffraction
chemical_list: D010741:Phospholipases A; D054467:Phospholipases A2