title: Presence and coding properties of 2'-O-methyl-5-carbamoylmethyluridine (ncm5Um) in the wobble position of the anticodon of tRNA(Leu) (U*AA) from brewer's yeast.
abstract: The unknown modified nucleoside U* has been isolated by enzymatic and HPLC protocols from tRNA(Leu) (U*AA) recently discovered in brewer's yeast. The pure U* nucleoside has been characterized by electron impact mass spectroscopy, and comparison of its chromatographic and UV-absorption properties with those of appropriate synthetic compounds. The structure of U* was established as 2'-O-methyl-5-carbamoylmethyluridine (ncm5Um). The yeast tRNA(Leu) (U*AA) is the only tRNA so far sequenced which has been shown to contain ncm5Um. The location of such a modified uridine at the first position of the anticodon restricts the decoding property to A of the leucine UUA codon.
authors: Laboratoire de Biochemie Médicale, Faculté de Médecine, Université de Bourgogne, Dijon, France.
authors: A L
authors: AL
authors: Glasser
authors: C
authors: C
authors: el Adlouni
authors: G
authors: G
authors: Keith
authors: E
authors: E
authors: Sochacka
authors: A
authors: A
authors: Malkiewicz
authors: M
authors: M
authors: Santos
authors: M F
authors: MF
authors: Tuite
authors: J
authors: J
authors: Desgrès
mesh_terms: D000926:Anticodon; D002851:Chromatography, High Pressure Liquid; D005656:Fungal Proteins / Q000096:biosynthesis; D013058:Mass Spectrometry; D015394:Molecular Structure; D012331:RNA, Fungal / Q000235:genetics; D012356:RNA, Transfer, Leu / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D013056:Spectrophotometry, Ultraviolet; D014529:Uridine / Q000031:analogs & derivatives* / Q000032:analysis / Q000737:chemistry / Q000235:genetics
chemical_list: D000926:Anticodon; D005656:Fungal Proteins; D012331:RNA, Fungal; D012356:RNA, Transfer, Leu; C013120:O(2)'-methyl-5-carbamoylmethyluridine; D014529:Uridine