Purification and amino-acid sequence of a nerve growth factor from the venom of Vipera russelli russelli.
Nerve growth factor (NGF) was purified from the venom of Vipera russelli russelli by Sephadex G-50 gel filtration, S-Sepharose column chromatography and Blue-Sepharose CL-6B column chromatography. The purified NGF was found to be a glycoprotein, whose apparent molecular mass was estimated to be about 17.5 kDa by SDS-PAGE. The amino-acid sequence was determined by a combination of conventional methods. The V. r. russelli NGF was composed of 117 amino-acid residues with one residue, Asn-21, being N-linked glycosylated and the molecular mass of its protein portion was calculated to be 13,280 Da.
Koyama
J
J
Department of Biochemistry, Osaka University of Pharmaceutical Sciences, Japan.
Inoue
S
S
Ikeda
K
K
Hayashi
K
K
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D003488:Cyanogen Bromide; D006031:Glycosylation; D008969:Molecular Sequence Data; D009414:Nerve Growth Factors / Q000737:chemistry / Q000302:isolation & purification*; D010455:Peptides / Q000032:analysis; D014757:Viper Venoms / Q000737:chemistry*
D000596:Amino Acids; D009414:Nerve Growth Factors; D010455:Peptides; D014757:Viper Venoms; D003488:Cyanogen Bromide