Membrane phospholipid composition in Saccharomyces uvarum cells grown in the presence of subinhibitory doses of amphotericin B and desertomycin.
In Saccharomyces uvarum cultures, subinhibitory doses of amphotericin B and desertomycin induced alterations in the plasma membrane phospholipid composition. Amphotericin B increased the lipid content in the plasma membrane. It showed a pronounced effect on fatty acyl constituents by raising the amounts of mono- and polyunsaturated derivatives in phospholipids. On the other hand, desertomycin had no apparent effect on fatty acid synthesis but altered the relative composition of phospholipids in the membrane. Phosphatidyl choline and phosphatidyl ethanolamine contents decreased, while their precursors, phosphatidyl serine and phosphatidic acid, increased. On the other hand, the rates of phosphatidyl inositol were not significantly affected by the action of either antifungal agent.
Benallaoua
S
S
Laboratoire de Biochimie microbienne, Faculté des Sciences pharmaceutiques et biologiques, Nancy, France.
Coulon
J
J
Bonaly
R
R
D000666:Amphotericin B / Q000494:pharmacology*; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002462:Cell Membrane / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D005227:Fatty Acids / Q000032:analysis / Q000378:metabolism; D066298:In Vitro Techniques; D018942:Macrolides; D008563:Membrane Lipids / Q000032:analysis / Q000378:metabolism*; D010743:Phospholipids / Q000032:analysis / Q000378:metabolism*; D012440:Saccharomyces / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism
D000900:Anti-Bacterial Agents; D005227:Fatty Acids; D018942:Macrolides; D008563:Membrane Lipids; D010743:Phospholipids; C042503:desertomycin; D000666:Amphotericin B