Bovine pancreatic kallikrein: purification and radioimmunoassay.
Kallikrein was isolated from bovine pancreatic tissue by a sequential procedure of anion-exchange, hydrophobic interaction and gel filtration chromatographies. A monospecific polyclonal antiserum to the pure enzyme was raised in rabbits and was used to set up a specific radioimmunoassay for bovine tissue kallikrein.
al-Hamidi
A A
AA
Department of Chemistry and Biological Chemistry, University of Essex, Colchester, UK.
al-Tufail
M A
MA
Bailey
G S
GS
D000818:Animals; D002417:Cattle; D002847:Chromatography, Agarose; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D007610:Kallikreins / Q000032:analysis / Q000302:isolation & purification*; D010179:Pancreas / Q000201:enzymology*; D011863:Radioimmunoassay / Q000379:methods* / Q000592:standards
D007610:Kallikreins