High-performance liquid chromatographic monitoring of metabolic products resulting from the treatment of mouse hepatoma cells with N6-cycloalkylated nucleosides.
High-performance liquid chromatography was used to analyze cell lysates and growth medium of mouse hepatoma cells, separately treated with N6-cyclopropyl-, N6-cyclobutyl-, and N6-cyclopentyladenosines, in an effort to gain insight into the mechanism by which these modified nucleosides exert their cytotoxic effect(s). The corresponding 5'-monophosphate of the respective modified nucleoside was detected in the separate cell lysate samples. Both the modified nucleoside and its corresponding 5'-monophosphate were detected in the separate growth medium samples and their relative concentrations therein were determined. These results indicate that the cytotoxicity of these N6-cycloalkylated nucleosides may be attributed to their 5'-monophosphates within the cells.
Thedford
R
R
Department of Chemistry, Clark Atlanta University, GA 30314.
Fauntleroy
L P
LP
Murray
Y L
YL
Stokes
M
M
Thornton
C L
CL
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D008114:Liver Neoplasms, Experimental / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D009705:Nucleosides / Q000378:metabolism / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D009705:Nucleosides