A simple, efficient method to create a cDNA library.
The simplified "All In One Tube" protocol for constructing a cDNA library combines the advantages of the "Classic method" and the Okayama-Berg method while overcoming some of their drawbacks. In this method, adding adapters, linkers or enzyme digestion steps are no longer necessary after cDNA synthesis, thus making it quicker and especially useful when dealing with small samples of mRNA.
Hu
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Department of Zoology, Michigan State University, East Lansing 48824.
Kopachik
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D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D016054:DNA, Protozoan / Q000235:genetics*; D005069:Evaluation Studies as Topic; D015723:Gene Library; D005821:Genetic Techniques; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D009253:Naegleria / Q000235:genetics
D016054:DNA, Protozoan