C-value paradox in angiosperm plant species. I. Sensitivity to DNase I in species with different 2C DNA content.
The experimental conditions for DNAase I digestion in situ for plant nuclei have been presented. Cytophotometric measurements of DNA loss performed on Feulgen-stained nuclei of three species differing in 2C DNA, heterochromatin and condensed euchromatin contents have shown that the lower 2C DNA amount the higher is DNase I sensitivity. Heterochromatin and some fractions of euchromatin are DNase I resistant. Microdensitometric measurements along M chromosome in Vicia faba have demonstrated the sites hypersensitive to DNase I.
Olszewska
M J
MJ
Department of Plant Cytology and Cytochemistry, University of Lódź, Poland.
D002453:Cell Cycle / Q000502:physiology; D002843:Chromatin / Q000187:drug effects / Q000378:metabolism; D002875:Chromosomes / Q000502:physiology; D004247:DNA / Q000187:drug effects* / Q000378:metabolism; D003720:Densitometry; D003850:Deoxyribonuclease I / Q000378:metabolism / Q000494:pharmacology*; D022041:Euchromatin; D006570:Heterochromatin / Q000187:drug effects / Q000378:metabolism; D008677:Metaphase / Q000502:physiology; D010944:Plants / Q000201:enzymology / Q000235:genetics*; D012680:Sensitivity and Specificity; D013045:Species Specificity
D002843:Chromatin; D022041:Euchromatin; D006570:Heterochromatin; D004247:DNA; D003850:Deoxyribonuclease I