[Rabbit annexins: comparison of a series of proteins in cell-free homogenates and membranes from various tissues and a method of rapid preliminary identification of individual components after two-dimensional electrophoresis].
The annexin sets in cell-free homogenates and membranes of rabbit skeletal and heart muscles, liver, kidney, lung, and brain, have been compared by one- and two-dimensional electrophoresis. The pIs and M(r)s of the proteins identified have been determined. The data on two-dimensional electrophoresis of annexins from different animals have been systematized. Simple graphs are proposed which allow to identify annexins on electrophoregrams. The technique has definite potentialities in recognition of some unidentified Ca(2+)-dependent membrane-binding proteins and may be used to predict the trend of search for novel members of the annexin family.
Mel'gunov
V I
VI
Nabokina
S M
SM
D000818:Animals; D017302:Annexins / Q000302:isolation & purification / Q000378:metabolism*; D002462:Cell Membrane / Q000737:chemistry; D002474:Cell-Free System; D015180:Electrophoresis, Gel, Two-Dimensional; D007526:Isoelectric Point; D011817:Rabbits; D014018:Tissue Distribution
D017302:Annexins
Anneksiny krolika: sravnenie nabora belkov v beskletochnykh gomogenatakh i membranakh razlichnykh tkaneĭ i sposob bystroĭ predvaritel'noĭ identifikatsii individual'nykh komponentov posle dvumernogoēlektroforeza.