title: [Plasmid transduction using the D3112DeltaH miniphage in Pseudomonas aeruginosa cells].
abstract: Plasmid DNA transduction with mini-D3112 delta H, deletion derivative of phage D3112, which lost the genes essential for phage growth but retained the sites required for transposition and packaging was studied. Unlike D3112, mini-D3112 delta H element can transduce plasmids and plasmid markers at frequencies of 10(-5)-10(-8) in rec+ cells of Pseudomonas aeruginosa. Plasmids R1162 and R388 of the size smaller than phage genome were transduced intact. Large plasmids, like RP4 and R151, were deleted under transduction. By this way, we isolated deletion derivatives of RP4. The smallest derivative pN2 contained a 4.5 kb fragment of RP4. Unlike the latter, pN2 plasmid had narrow host range and did not maintain in Escherichia coli cells.
authors: O V
authors: OV
authors: Mar'ina
authors: T V
authors: TV
authors: Gerasimova
authors: A O
authors: AO
authors: Bekkarevich
authors: E A
authors: EA
authors: Khrenova
authors: A S
authors: AS
authors: Ianenko
mesh_terms: D001435:Bacteriophages / Q000235:genetics*; D004279:DNA, Viral / Q000648:ultrastructure; D004587:Electrophoresis, Agar Gel; D017353:Gene Deletion; D008854:Microscopy, Electron; D010957:Plasmids; D011550:Pseudomonas aeruginosa / Q000235:genetics*; D015183:Restriction Mapping; D014161:Transduction, Genetic
chemical_list: D004279:DNA, Viral
vernacular_title: Transduktsiia plazmid s pomoshch'iu minifaga D3112DeltaH v kletkakh Pseudomonas aeruginosa.