Tissue distribution of intraperitoneally administered (1-->3)-beta-D-glucan (SSG), a highly branched antitumor glucan, in mice.
Distribution of metabolically 3H-labeled (1-->3)-beta-D-glucan (3H-SSG) obtained from the culture filtrate of Sclerotinia sclerotiorum IFO 9395, in various tissues after intraperitoneal administration into ICR mice (250 micrograms/mouse) was examined. 3H-SSG was mainly detected in liver, spleen, and blood, and a negligible amount was excreted into the feces and excrement within 2 d. The significant amount of 3H-SSG remained in liver and spleen after 1 month. On the other hand, SSG was not incorporated effectively in vivo and in vitro by peritoneal exudate macrophages (0.5 microgram/1 x 10(6) M phi) Similarly in vivo, the majority of 3H-SSG distributed in spleen and liver were recovered from the non-cellular fraction and not from splenic macrophage and Kupffer cell fractions. These results suggested that (1-->3)-beta-D-glucans would not be easily incorporated by the host cells to degrade and exclude from the body even after the onset of the biological response modifier activity.
Suda
M
M
Laboratory of Immunopharmacology of Microbial Products, Tokyo College of Pharmacy, Japan.
Ohno
N
N
Adachi
Y
Y
Yadomae
T
T
D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000097:blood / Q000493:pharmacokinetics*; D001203:Ascomycota; D005936:Glucans / Q000008:administration & dosage / Q000097:blood / Q000493:pharmacokinetics*; D007274:Injections, Intraperitoneal; D008099:Liver / Q000166:cytology / Q000378:metabolism; D008264:Macrophages / Q000378:metabolism; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D013154:Spleen / Q000166:cytology / Q000378:metabolism; D014018:Tissue Distribution; D047071:beta-Glucans
D000970:Antineoplastic Agents; D005936:Glucans; D047071:beta-Glucans; C033363:beta-1,3-glucan