Long term incubation of cardiac myocytes with oleic acid and very-low density lipoprotein reduces heparin-releasable lipoprotein lipase activity.
An exogenous [3H]triolein emulsion was hydrolyzed by intact cardiac myocytes with functional LPL located on the cell surface. This surface-bound LPL could be released into the medium when cardiac myocytes were incubated with heparin. Incubation of cardiac myocytes with VLDL, or the products of TG breakdown, oleic acid or 2-monoolein, did not increase LPL activity in the medium. However, incubation of cardiac myocytes with either VLDL or oleic acid for > 60 min did reduce heparin-releasable LPL activity. In the heart, this inhibitory effect of FFA could regulate the translocation of LPL from its site of synthesis in the cardiac myocyte to its functional site at the capillary endothelium.
Rodrigues
B
B
Department of Pharmacology & Therapeutics, Faculty of Medicine, University of Calgary, Alberta, Canada.
Spooner
M R
MR
Severson
D L
DL
D000818:Animals; D002462:Cell Membrane / Q000187:drug effects; D002478:Cells, Cultured; D005230:Fatty Acids, Nonesterified / Q000494:pharmacology*; D005989:Glycerides / Q000494:pharmacology*; D006493:Heparin / Q000494:pharmacology; D008066:Lipolysis; D008071:Lipoprotein Lipase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D008079:Lipoproteins, VLDL / Q000494:pharmacology*; D008565:Membrane Proteins / Q000037:antagonists & inhibitors* / Q000378:metabolism; D009206:Myocardium / Q000166:cytology / Q000201:enzymology*; D019301:Oleic Acid; D009829:Oleic Acids / Q000494:pharmacology*; D051381:Rats; D014304:Triolein / Q000378:metabolism*
D005230:Fatty Acids, Nonesterified; D005989:Glycerides; D008079:Lipoproteins, VLDL; D008565:Membrane Proteins; D009829:Oleic Acids; D014304:Triolein; D019301:Oleic Acid; D006493:Heparin; C005953:monoolein; D008071:Lipoprotein Lipase