The anti-tumor effect of a small polypeptide from Coriolus versicolor (SPCV).
A new small polypeptide was isolated from the crude extraction of polysaccharide peptide of Coriolus versicolor (Cov-1) by HPLC and CIEF. It has a smaller molecular weight (10K) compared with that of PSP (100K) and was named small peptide of Coriolus versicolor, SPCV. It was found that SPCV possesses potent cytotoxic effect on human tumor cell lines of HL-60, LS174-T, SMMU-7721, and SCG-7901. The IC50 of SPCV on HL-60 was 30 micrograms/ml. The inhibition rates of leukemia cells and SCG-7901 were significantly higher in SPCV treated group than that in PSP and PSK groups. SPCV also has immunopotentiating effect as it increased WBC and IgG levels. Pretreatment of SPCV for two weeks decreased the incidence of tumor mass in nude mice inoculated with tumor cells.
Yang
M M
MM
Department of Physiology, University of Hong Kong.
Chen
Z
Z
Kwok
J S
JS
D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000627:therapeutic use; D001487:Basidiomycota / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D004353:Drug Evaluation, Preclinical; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D007074:Immunoglobulin G / Q000187:drug effects; D007525:Isoelectric Focusing; D007962:Leukocytes / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D008970:Molecular Weight; D009374:Neoplasms, Experimental / Q000188:drug therapy* / Q000473:pathology; D010455:Peptides / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D011509:Proteoglycans / Q000627:therapeutic use; D014407:Tumor Cells, Cultured / Q000187:drug effects
D000903:Antibiotics, Antineoplastic; D004365:Drugs, Chinese Herbal; D007074:Immunoglobulin G; D010455:Peptides; D011509:Proteoglycans; C010770:polysaccharide-K