Measurement of glycated albumin by the nitroblue tetrazolium colorimetric method.
A method has been developed for the measurement of glycated albumin (albumin-fructosamine) by the nitroblue tetrazolium (NBT) colorimetric method. In this method, polyethylene glycol was added to the serum and then the mixture was centrifuged to separate globulin proteins from albumin proteins. This made it possible to measure the glycated albumin in the supernatant by the NBT colorimetric method, without the interference of globulin proteins. This measurement method correlated with the measurement of glycated albumin using boronate affinity chromatography with an r value of 0.942 (P < 0.001). Our method using polyethylene glycol permits easy measurement of albumin fructosamine and is therefore useful as an index of diabetic control and for diabetic screening.
Mashiba
S
S
Kyoto Medical Science Laboratory Incorporation, Japan.
Uchida
K
K
Okuda
S
S
Tomita
S
S
D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D003124:Colorimetry; D003922:Diabetes Mellitus, Type 1 / Q000097:blood; D003924:Diabetes Mellitus, Type 2 / Q000097:blood; D019270:Fructosamine; D005916:Globulins / Q000302:isolation & purification; D017127:Glycation End Products, Advanced; D006595:Hexosamines / Q000032:analysis; D006801:Humans; D009580:Nitroblue Tetrazolium; D010084:Oxidation-Reduction; D011092:Polyethylene Glycols; D012016:Reference Values; D012709:Serum Albumin / Q000378:metabolism*
D005916:Globulins; D017127:Glycation End Products, Advanced; D006595:Hexosamines; D012709:Serum Albumin; D009580:Nitroblue Tetrazolium; D011092:Polyethylene Glycols; D019270:Fructosamine