[Study on L-form induction of C. albicans].
After culturing C. albicans with ketoconazole on high osmosing medium containing thioglycollate, pig serum and sucrose, a cell wall-deficient strain of C. albicans was obtained. We compared its physiologic and biochemical characteristics with those of the parent strain. This cell wall-deficient strain not only changed its morphology but also the chemical components of the cell wall. The cell wall-deficient strain lacked 4 characteristic protein bands and had cell wall mannan decreased to 44% of that of the parent strain. Animal models revealed differences between the toxicities of the two strains: the mean survival time of mice inoculated intravenously with 10(6) deficient strain cells was 23.5 days (70% of the mice died within 30 days), but that of mice injected with 10(6) parent strain cells was 15.8 days (100% of the mice died in 30 days).
Xi
L
L
Institute of Dermatology, Nanjing.
D000818:Animals; D002176:Candida albicans / Q000502:physiology*; D002473:Cell Wall / Q000737:chemistry / Q000648:ultrastructure; D005656:Fungal Proteins / Q000032:analysis; D007654:Ketoconazole / Q000494:pharmacology; D007740:L Forms / Q000166:cytology / Q000472:pathogenicity; D008351:Mannans / Q000032:analysis; D051379:Mice; D014774:Virulence
D005656:Fungal Proteins; D008351:Mannans; D007654:Ketoconazole