Cell growth on immobilized cell-growth factor; 4: Interaction of fibroblast cells with insulin immobilized on poly(methyl methacrylate) membrane.
Insulin was immobilized on the surface-hydrolyzed poly(methyl methacrylate) membrane and the growth acceleration of mouse fibroblast cells, STO, by the immobilized insulin was investigated. It was found that insulin remains immobilized on the surface of nonbiodegradable membrane and interacts specifically with receptors existing on the biological membrane of fibroblast cells. The growth acceleration by immobilized insulin was enhanced by introduction of a spacer arm between insulin and the immobilization matrix. The amount of receptor proteins present on the biological membrane of fibroblast cells after culturing with insulin, immobilized on nonbiodegradable polymer membrane, was much higher than that after culturing with free insulin, implying the suppression of down-regulation in the case of immobilized insulin.
Liu
S Q
SQ
Department of Polymer Chemistry, Faculty of Engineering, Kyoto University, Japan.
Ito
Y
Y
Imanishi
Y
Y
D000818:Animals; D002455:Cell Division / Q000187:drug effects*; D002478:Cells, Cultured; D015536:Down-Regulation; D005347:Fibroblasts / Q000166:cytology; D006133:Growth Substances / Q000494:pharmacology; D007328:Insulin / Q000032:analysis / Q000494:pharmacology*; D007457:Iodine Radioisotopes; D008567:Membranes, Artificial; D020366:Methylmethacrylate; D008768:Methylmethacrylates; D051379:Mice; D011972:Receptor, Insulin / Q000032:analysis
D006133:Growth Substances; D007328:Insulin; D007457:Iodine Radioisotopes; D008567:Membranes, Artificial; D008768:Methylmethacrylates; D020366:Methylmethacrylate; D011972:Receptor, Insulin