Lymphocyte transformation test for house dust mite in atopic dermatitis: relationship between mite antigens for type I and type IV allergy.
Using a crude extract obtained from Dermatophagoides farinae, and its four fractions (I, II, III and IV) partially purified by high-speed gel filtration chromatography, scratch tests and lymphocyte transformation tests (LTTs) were performed on 37 patients with atopic dermatitis. Crude mite antigen provoked positive scratch test reactions in 25 (68%), and positive LTT reactions in 19 (51%) of the 37 patients examined. Mite antigen fractions I, II, III and IV induced a positive LTT reaction in 19, 22, 17 and 7 patients, respectively. Of the 65 positive LTT reactions, 25 accompanied a positive scratch test to the antigen fraction which provoked the positive LTT reaction, but 40 accompanied a negative scratch test. These findings suggest that house dust mite antigen fractions for type IV allergy are different from those for type I allergy in a considerable proportion of patients with atopic dermatitis.
Sasaki
K
K
Department of Dermatology, Shiga University of Medical Science, Otsu, Japan.
Sugiura
H
H
Uehara
M
M
D000293:Adolescent; D000328:Adult; D000485:Allergens / Q000276:immunology*; D000818:Animals; D039741:Antigens, Dermatophagoides; D002648:Child; D003876:Dermatitis, Atopic / Q000175:diagnosis* / Q000276:immunology; D005260:Female; D006801:Humans; D006968:Hypersensitivity, Delayed / Q000175:diagnosis* / Q000276:immunology; D008213:Lymphocyte Activation; D008297:Male; D008875:Middle Aged; D008925:Mites / Q000276:immunology*; D012882:Skin Tests
D000485:Allergens; D039741:Antigens, Dermatophagoides