Role of cell-mediated immunity in the resistance to experimental sporotrichosis in mice.
Congenitally athymic nude (nu/nu) mice showed higher sensitivity to intratestical infection of Sporothrix schenckii than phenotypically normal littermates (nu/+). Active immunization with viable cells enhanced the resistance to intravenous (i.v.) infection of this fungus in BALB/c mice. Nu/nu mice transferred with immune spleen cells acquired the enhancement of resistance to the infection with S. schenckii, but not ones with normal spleen cells. Pre-treatment of OK-432 (Picibanil), one of the macrophage activating agents, enhanced resistance to i.v. infection of this fungus in BALB/c mice. On the other hand, pretreatment of carrageenan, one of the macrophage inhibitors, impaired the resistance. This fungus was intracellularly killed by peritoneal macrophage from OK-432 treated or immunized mice but not by those from normal mice in vitro. These results suggest that activated macrophage, that was mediated by T cells, play an important role in the resistance to S. schenckii in mice.
Shiraishi
A
A
Bioscience Research Laboratories, Sankyo Co., Ltd., Tokyo, Japan.
Nakagaki
K
K
Arai
T
T
D000818:Animals; D002351:Carrageenan / Q000494:pharmacology; D007111:Immunity, Cellular / Q000276:immunology; D007113:Immunity, Innate / Q000187:drug effects / Q000276:immunology; D016219:Immunotherapy, Adoptive; D008264:Macrophages / Q000276:immunology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D010844:Picibanil / Q000494:pharmacology; D013154:Spleen / Q000276:immunology; D013175:Sporothrix / Q000254:growth & development / Q000276:immunology*; D013174:Sporotrichosis / Q000276:immunology*; D014611:Vaccination
D010844:Picibanil; D002351:Carrageenan