Transcriptional inhibition of the bacteriophage T7 early promoter region by oligonucleotide triple helix formation.
We have identified a purine-rich triplex binding sequence overlapping a -35 transcriptional early promoter region of the bacteriophage T7. Triplex-forming oligonucleotide designed to bind this target was annealed to T7 templates and introduced into in vitro transcription systems under conditions favoring specific initiation from this promoter. These templates demonstrated significant transcriptional inhibition relative to naked genomic templates and templates mixed with non-triplex-forming oligonucleotide. It is suggested that triplex formation along this target interferes with transcriptional initiation, and this mechanism may hold potential to disrupt bacteriophage T7 early transcription in vivo.
Ross
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C
Department of Biology, West Chester University, PA 19383.
Samuel
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M
Broitman
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SL
D017123:Bacteriophage T7 / Q000187:drug effects / Q000235:genetics*; D001483:Base Sequence; D007700:Kinetics; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis / Q000494:pharmacology*; D011401:Promoter Regions, Genetic / Q000187:drug effects*; D012367:RNA, Viral / Q000096:biosynthesis / Q000235:genetics; D013698:Templates, Genetic; D014158:Transcription, Genetic / Q000187:drug effects*
D009838:Oligodeoxyribonucleotides; D012367:RNA, Viral