Determination of vitamin C and organic acids in various fruits by HPLC.
Liquid chromatographic methodology for determination of vitamin C and organic acids in different fruits (whortleberry, blackberry, red currant, black currant, raspberry, babaco, feijoa, kiwano, passion fruit, red and yellow tamarillos, medlar, and persimmon) cultivated in Galicia, Spain are developed. Both methods use a C18 column and an RP-18 precolumn for the stationary phase. High-performance-liquid-chromatographic-(HPLC-) grade water is brought to pH 2.2 with sulphuric acid for the mobile phase. The flow rate is 0.4 mL/min, and UV detection is at 254 nm for vitamin C and 214 nm for organic acids. Coefficients of variation of 1.84, 1.20, 1.00, and 2.60% are obtained for the vitamin C, quinic acid, malic acid and citric acid, respectively.
Romero Rodriguez
M A
MA
Departamento de Química Analítica, Nutrición y Bromatología, Facultad de Farmacia, La Coruña, Spain.
Vazquez Oderiz
M L
ML
Lopez Hernandez
J
J
Simal Lozano
J
J
D001205:Ascorbic Acid / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002951:Citrates / Q000032:analysis*; D005638:Fruit / Q000737:chemistry*; D008293:Malates / Q000032:analysis*; D011801:Quinic Acid / Q000032:analysis*
D002951:Citrates; D008293:Malates; D011801:Quinic Acid; D001205:Ascorbic Acid