Three electrophoretic techniques comparison for des-gamma-carboxyprothrombin detection.
Des-gamma-carboxyprothrombin (DCP) is a marker that appears in the blood when modifications of vitamin K-dependent proteins carboxylation cycle occur. About 280 human plasma samples of diverse origins were tested by three different electrophoretic techniques for the evaluation of DCP: rocket immunoelectrophoresis (RIE) before and after barium carbonate adsorption, crossed affinoimmunoelectrophoresis (CAIE) and polyacrylamide gel electrophoresis in presence of calcium lactate followed by immunoblotting (PAGE-blot). A good correlation was found between CAIE and PAGE-blot in the CAIE detection limit, but not between RIE and the two other techniques. PAGE-blot was more sensitive than RIE and CAIE and allowed reliable quantification of abnormal prothrombin in plasma.
Belle
M
M
Institut Pasteur de Lyon, Laboratoire de Micro-Analyse et Nutrition, France.
Guillaumont
M
M
Leclercq
M
M
Guinet
R
R
D000327:Adsorption; D000328:Adult; D000925:Anticoagulants / Q000494:pharmacology; D001464:Barium; D015415:Biomarkers / Q000097:blood; D001797:Blood Protein Electrophoresis / Q000379:methods*; D002254:Carbonates; D003550:Cystic Fibrosis / Q000097:blood; D004591:Electrophoresis, Polyacrylamide Gel; D005069:Evaluation Studies as Topic; D006801:Humans; D007122:Immunoelectrophoresis; D007123:Immunoelectrophoresis, Two-Dimensional; D007231:Infant, Newborn / Q000097:blood; D008107:Liver Diseases / Q000097:blood; D011498:Protein Precursors; D011516:Prothrombin / Q000031:analogs & derivatives* / Q000032:analysis; D012680:Sensitivity and Specificity; D014813:Vitamin K Deficiency / Q000097:blood*
D000925:Anticoagulants; D015415:Biomarkers; D002254:Carbonates; D011498:Protein Precursors; D001464:Barium; C008208:acarboxyprothrombin; C006685:barium carbonate; D011516:Prothrombin