title: Gas-liquid chromatographic analysis of volatile short chain fatty acids in fecal samples as pentafluorobenzyl esters.
abstract: A protocol was developed for the analysis of volatile short chain fatty acids in microsamples of feces, short chain fatty acid (SCFA) extraction was from fecal samples using ethanol incorporating n-hexanoic acid as an internal standard. The SCFAs were converted to pentafluorobenzyl esters with alpha-2,3,4,5,6-pentafluorotoluene and analyzed on a gas-liquid chromatograph equipped with an electron capture detector. One hundred milligrams of sample was routinely used but analysis could be carried out on 20 mg of sample.
authors: Department of Therapeutics and Pharmacology, Queen's University of Belfast, Northern Ireland, United Kingdom.
authors: L T
authors: LT
authors: McGrath
authors: C D
authors: CD
authors: Weir
authors: S
authors: S
authors: Maynard
authors: B J
authors: BJ
authors: Rowlands
mesh_terms: D000818:Animals; D001565:Benzoates; D002208:Caproates; D002849:Chromatography, Gas / Q000379:methods; D004952:Esters; D005230:Fatty Acids, Nonesterified / Q000032:analysis*; D005243:Feces / Q000737:chemistry*; D007202:Indicators and Reagents; D008297:Male; D051381:Rats; D017208:Rats, Wistar
chemical_list: D001565:Benzoates; D002208:Caproates; D004952:Esters; D005230:Fatty Acids, Nonesterified; D007202:Indicators and Reagents; C037652:hexanoic acid