title: Fatty acid composition and properties of the liver microsomal membrane of rats fed diets enriched with cholesterol.
abstract: Male rats were fed diets containing olive (OO) or evening primrose (EPO) oil (10% w/w), with or without added cholesterol (1% w/w). After 6-week feeding, the lipid and fatty acid compositions, fluidity, and fatty acid desaturating and cholesterol biosynthesis/esterification related enzymes of liver microsomes were determined. Both the OO and EPO diets, without added cholesterol, increased the contents of oleic and arachidonic acids, respectively, of rat liver microsomes. The results were consistent with the increases in delta 9 and delta 6 desaturation of n-6 essential fatty acids and the lower microviscosity in the EPO group. Dietary cholesterol led to an increase in the cholesterol content of liver microsomes as well as that of phosphatidylcholine (PC). The cholesterol/phospholipid and PC/PE (phosphatidylethanolamine) ratios were also elevated. Fatty acid composition changes were expressed as the accumulation of monounsaturated fatty acids, with accompanying milder depletion of saturated fatty acids in rat liver microsomes. In addition, the arachidonic acid content was lowered, with a concomitant increase in linoleic acid, which led to a significant decrease in the 20:4/18:2 ratio in comparison to in animals fed the cholesterol-free diets. Cholesterol feeding also increased delta 9 desaturase activity as well as membrane microviscosity, whereas it decreased delta 6 and delta 5 desaturase activities. There was a very strong correlation between fluidity and the unsaturation index reduction in the membrane. Furthermore, the activity of hydroxymethylglutaryl-CoA reductase increased and the activity of acyl-CoA:cholesterol acyltransferase decreased in liver microsomes from both cholesterol-fed groups.(ABSTRACT TRUNCATED AT 250 WORDS)
authors: Instituto de la Grasa y sus Derivados (CSIC), Sevilla, Spain.
authors: F J
authors: FJ
authors: Muriana
authors: C M
authors: CM
authors: Vazquez
authors: V
authors: V
authors: Ruiz-Gutierrez
mesh_terms: D000818:Animals; D001835:Body Weight / Q000187:drug effects; D002791:Cholesterol, Dietary / Q000378:metabolism / Q000494:pharmacology*; D000090943:Delta-5 Fatty Acid Desaturase; D004435:Eating / Q000187:drug effects; D044943:Fatty Acid Desaturases / Q000187:drug effects / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism*; D005228:Fatty Acids, Essential / Q000378:metabolism / Q000494:pharmacology; D005454:Fluorescence Polarization; D005456:Fluorescent Dyes; D006903:Hydroxymethylglutaryl CoA Reductases / Q000187:drug effects / Q000378:metabolism; D007425:Intracellular Membranes / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism; D008041:Linoleic Acids; D050602:Linoleoyl-CoA Desaturase; D050356:Lipid Metabolism; D008099:Liver / Q000033:anatomy & histology / Q000187:drug effects; D008297:Male; D008560:Membrane Fluidity / Q000187:drug effects; D008862:Microsomes, Liver / Q000737:chemistry / Q000187:drug effects* / Q000201:enzymology; D031665:Oenothera biennis; D000069463:Olive Oil; D009929:Organ Size / Q000187:drug effects; D010938:Plant Oils / Q000378:metabolism / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D013230:Stearoyl-CoA Desaturase; D002785:Sterol O-Acyltransferase / Q000187:drug effects / Q000378:metabolism; D017965:gamma-Linolenic Acid
chemical_list: D002791:Cholesterol, Dietary; D000090943:Delta-5 Fatty Acid Desaturase; D005227:Fatty Acids; D005228:Fatty Acids, Essential; D005456:Fluorescent Dyes; D008041:Linoleic Acids; D000069463:Olive Oil; D010938:Plant Oils; C028498:evening primrose oil; D017965:gamma-Linolenic Acid; D006903:Hydroxymethylglutaryl CoA Reductases; D044943:Fatty Acid Desaturases; D013230:Stearoyl-CoA Desaturase; D050602:Linoleoyl-CoA Desaturase; C029627:delta-9 fatty acid desaturase; D002785:Sterol O-Acyltransferase