Effects of sex steroids on cell differentiation and interleukin-1 beta production in the human promyelocytic leukemia cell line HL-60.
The effects of 17 beta-estradiol (E2), progesterone (P) and testosterone (Te) on cell differentiation in the HL-60 promyelocytic leukemia cells after treatment with 1 alpha, 25-dihydroxyvitamin D3 [1,25-(OH)2D3] and those on interleukin-1 beta (IL-1 beta) production by HL-60 cells in response to lipopolysaccharide (LPS) were investigated. Neither E2 (10(-10) to 10(-7) M), P (10(-9) to 10(-6) M) nor Te (10(-10) to 10(-7) M) affected monocytic differentiation as assessed by reactivity with OKM14 monoclonal antibody and alpha-naphthyl acetate esterase activity. Pretreatment of HL-60 cells with 1,25-(OH)2D3 enhanced their ability to produce IL-1 beta in response to subsequent exposure to LPS, although 1,25-(OH)2D3 by itself did not induce IL-1 beta production by HL-60 cells. This priming effect of 1,25-(OH)2D3 was augmented by the addition of E2 and Te at physiologic concentrations, but not by that of P. E2, P and Te at physiologic concentrations enhanced IL-1 beta production by HL-60 cells that were pretreated with 1,25(OH)2D3 and stimulated by LPS. The increasing rate of IL-1 beta production by the addition of E2 and Te was higher when added with LPS than when added with 1,25-(OH)2D3. These findings suggest that enhancing effects of sex steroids in IL-1 beta production by monocyte/macrophage lineage cells.
Mori
H
H
Department of Obstetrics and Gynecology, Gifu University School of Medicine, Japan.
Sawairi
M
M
Itoh
N
N
Hanabayashi
T
T
Tamaya
T
T
D000704:Analysis of Variance; D002117:Calcitriol / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D004958:Estradiol / Q000494:pharmacology; D012739:Gonadal Steroid Hormones / Q000494:pharmacology*; D006801:Humans; D007375:Interleukin-1 / Q000096:biosynthesis*; D015473:Leukemia, Promyelocytic, Acute / Q000188:drug therapy* / Q000378:metabolism / Q000503:physiopathology; D008070:Lipopolysaccharides / Q000494:pharmacology; D009283:Naphthol AS D Esterase / Q000096:biosynthesis; D011374:Progesterone / Q000494:pharmacology; D013739:Testosterone / Q000494:pharmacology; D013997:Time Factors; D014407:Tumor Cells, Cultured
D012739:Gonadal Steroid Hormones; D007375:Interleukin-1; D008070:Lipopolysaccharides; D013739:Testosterone; D011374:Progesterone; D004958:Estradiol; D009283:Naphthol AS D Esterase; D002117:Calcitriol