Afferent and efferent arms of the humoral immune response to CSF-administered albumins in a rat model with normal blood-brain barrier permeability.
Cerebrospinal fluid (CSF) and serum antibody responses to albumin administered into CSF or muscle have been compared with respect to titer, isotype profile and complement-fixing activity in a rat model with normal brain barrier function. CSF/serum titer ratios and the ratio of IgG subclasses, IgG1/IgG2, were both elevated following CSF immunization. In contrast, there was no difference in complement-fixing activity between antibodies elicited by the two routes of immunization. It is suggested that intrathecal antibody synthesis accounts for the elevated CSF antibody titers in CSF-immunized rats, providing the first example of central nervous system antibody synthesis in an animal with normal brain barrier permeability.
Cserr
H F
HF
Section of Physiology, Brown University, Providence, RI 02912.
DePasquale
M
M
Harling-Berg
C J
CJ
Park
J T
JT
Knopf
P M
PM
D000418:Albumins / Q000276:immunology; D000818:Animals; D000867:Anterior Chamber / Q000276:immunology; D000906:Antibodies / Q000134:cerebrospinal fluid*; D000917:Antibody Formation; D001812:Blood-Brain Barrier; D003167:Complement Activation; D007114:Immunization; D007132:Immunoglobulin Isotypes / Q000032:analysis; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
D000418:Albumins; D000906:Antibodies; D007132:Immunoglobulin Isotypes