Reduction in the susceptibility of Aedes aegypti to Brugia malayi infection after treatment with ethyl methanesulfonate.
The susceptibility to Brugia malayi infections of F2 and F4 progenies of Aedes aegypti (Black Eye strain) treated with ethyl methanesulfonate (EMS) was tested. Both 3-day-old males and females were treated with 0.025, 0.050, 0.075 and 0.10% EMS. Control and treated females were then mated with normal or treated males to recover F1 progeny. F2 offspring were derived from sibling intercrosses, and 3 lines were established by further intercross matings to generate the F4. Susceptibility in the 0.025, 0.050, 0.075 and 0.10% EMS F2s was reduced by 13, 12, 4 and 25%, respectively. The 0.025% and 0.050% EMS F2 females showed a 29 to 39% decrease in mean L3 numbers. At 0.075 and 0.10% EMS, mean L3 numbers decreased by 0.8 and 71%, respectively. The F4 populations gave overall infections of 65, 56 and 22% for the control, 0.25 and 0.10% EMS lines, respectively. Mean L3s were reduced by 24 and 77%, respectively, in the 0.025 and 0.10% F4 EMS-selected populations.
Rodriguez
P H
PH
Division of Life Sciences, University of Texas, San Antonio 78249.
Lazaro
C
C
Castillon
R
R
D000330:Aedes / Q000469:parasitology*; D000818:Animals; D017178:Brugia malayi; D005020:Ethyl Methanesulfonate; D005260:Female; D007814:Larva; D008297:Male
D005020:Ethyl Methanesulfonate