Induction of contact sensitivity by cell-associated immunocomplexes requires activation of the early complement components.
Lymph node cells collected from CBA/J mice 4 days after painting the skin with picryl chloride behave like antigen presenting cells and induce contact sensitivity when injected into naive recipient mice. The immunizing capacity of these '4-day' cells is due to T cells which carry on their membrane hapten-IgM immunocomplexes. Incubation of the cells with complement from mouse strains that express high C4 serum levels (C4H), abolishes their immunizing capacity. This effect is related to the activation of the early components of the classical complement pathway, as supported by experiments using C3 and C4-depleted or C5 and C6-genetically deficient mouse sera. The detection of different amounts of C3b and C4b on the surface of 4-day T cells after incubation with C4L and C4H sera supports the possibility that membrane bound activated complement components could modify the immunizing capacity of these cells. Results herein reported suggest that membrane-bound C3b and C4b are not per se inhibitory but interfere with the residual complement activating capacity of 4-day T cells. The role of complement activation by 4-day T cells is pivotal as complement depletion of recipient mice by cobra venom factor (CVF) inhibits the immunizing capacity of untreated 4-day T cells, while 4-day T cells treated with complement in vitro and injected together with C4a anaphylatoxin are able to immunize recipient mice.
Lio
D
D
Institute of General Pathology, University of Palermo, Italy.
Sireci
G
G
Gervasi
F
F
Dieli
F
F
Salerno
A
A
D000818:Animals; D000936:Antigen-Antibody Complex / Q000276:immunology*; D000938:Antigen-Presenting Cells / Q000276:immunology; D003179:Complement C3b / Q000276:immunology; D015935:Complement C4b / Q000276:immunology; D003171:Complement Pathway, Classical / Q000276:immunology*; D003877:Dermatitis, Contact / Q000276:immunology*; D007075:Immunoglobulin M / Q000276:immunology; D008198:Lymph Nodes / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008808:Mice, Inbred CBA; D013601:T-Lymphocytes / Q000276:immunology
D000936:Antigen-Antibody Complex; D007075:Immunoglobulin M; D003179:Complement C3b; D015935:Complement C4b