High-performance liquid chromatographic determination of dihydroorotate dehydrogenase of Plasmodium falciparum and effects of antimalarials on enzyme activity.
A reversed-phase high-performance liquid chromatographic technique for the determination of dihydroorotate dehydrogenase in Plasmodium falciparum was developed. The assay was applied to the evaluation of the effects of several antimalarial drugs on the enzyme. Treatment of both the asexual and gametocyte stages of P. falciparum in culture with menoctone, primaquine or the primaquine derivative WR 238605 led to depression of the enzyme activity, although the drugs did not appear to inhibit the enzyme directly.
Ittarat
I
I
Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok, Thailand.
Webster
H K
HK
Yuthavong
Y
Y
D000634:Aminoquinolines / Q000494:pharmacology; D000818:Animals; D000962:Antimalarials / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D000090722:Dihydroorotate Dehydrogenase; D004789:Enzyme Activation / Q000187:drug effects; D009285:Naphthoquinones / Q000494:pharmacology; D010088:Oxidoreductases / Q000032:analysis* / Q000378:metabolism* / Q000502:physiology; D044925:Oxidoreductases Acting on CH-CH Group Donors; D010963:Plasmodium falciparum / Q000201:enzymology*; D011319:Primaquine / Q000494:pharmacology
D000634:Aminoquinolines; D000962:Antimalarials; D000090722:Dihydroorotate Dehydrogenase; D009285:Naphthoquinones; C055852:tafenoquine; D010088:Oxidoreductases; D044925:Oxidoreductases Acting on CH-CH Group Donors; D011319:Primaquine; C001697:menoctone