In vivo studies on the effects of immunity genes on early lytic transcription in the Halobacterium salinarium phage phi H.
We have studied in vivo the effects of putative immunity genes on the expression of an early lytic gene of the Halobacterium salinarium phage. phi H. We transformed an H. salinarium host with DNA coding for a putative repressor gene, the transcript of which has been designated T6. We show that, in vivo, this gene specifically shuts off production of the early lytic transcript T4. A construct carrying the DNA transcribed as T4, but without its putative repressor binding sequences, shows T4 transcription enhanced to a level comparable to that observed in lytic growth of mutant phages capable of growing on immune H. salinarium strains. This transcript is insensitive to the action of the T6 product. The product of this 'unrepressed' T4 transcript is able to complement in trans the repressed T4 on superinfecting phi H-sensitive phages, allowing these to grow on a strain containing the repressor gene. It has, however, no effect on the production of repressor. We also mapped the start and end points of two other transcripts, T9 and T10, which are expressed only in the lysogenic state by cells immune to superinfection by phage, cloned the coding DNA and used it to transform H. salinarium. This DNA, though transcribed by the transformants, has no detectable effect on the cells, which remain susceptible to phage infection.
Stolt
P
P
Max-Planck-Institut für Biochemie, Martinsried, FRG.
Zillig
W
W
D001435:Bacteriophages / Q000235:genetics* / Q000276:immunology; D001483:Base Sequence; D001665:Binding Sites; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification; D005814:Genes, Viral; D006217:Halobacterium / Q000235:genetics* / Q000276:immunology; D008242:Lysogeny; D008969:Molecular Sequence Data; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification; D012097:Repressor Proteins / Q000378:metabolism; D015183:Restriction Mapping; D014158:Transcription, Genetic
D004279:DNA, Viral; D012367:RNA, Viral; D012097:Repressor Proteins