Pre- and postnatal protein undernutrition increases hepatic carnitine palmitoyltransferase I activity and decreases enzyme sensitivity to inhibitors in the suckling rat.
Rats were pair-fed isocaloric diets containing either 25% (control diet) or 6% protein (low-protein diet) during the 5 weeks prior to conception and through the gestation and lactation periods; then, carnitine palmitoyltransferase I (CPT-I) activity was determined in liver and skeletal muscle mitochondria isolated from the corresponding pups. Maternal protein undernutrition increased the activity of hepatic CPT-I all along the suckling period, whereas the activity of the skeletal muscle enzyme was unaffected. Moreover, the sensitivity of hepatic CPT-I to inhibition by both malonyl-CoA and 4-hydroxyphenylglyoxylate was decreased in the low-protein group. These alterations in the properties of hepatic CPT-I may be involved in the appearance of hyperketonemia in the rat pup upon maternal administration of low-protein diets.
Guzmán
M
M
Department of Biochemistry and Molecular Biology I, Faculty of Chemistry, Complutense University, Madrid, Spain.
Azzolin
I R
IR
Moulin
C C
CC
Perry
M L
ML
D000818:Animals; D002334:Carnitine O-Palmitoyltransferase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D005260:Female; D006038:Glyoxylates / Q000494:pharmacology*; D007700:Kinetics; D007774:Lactation; D008316:Malonyl Coenzyme A / Q000494:pharmacology*; D008431:Maternal-Fetal Exchange; D008930:Mitochondria, Liver / Q000201:enzymology*; D008931:Mitochondria, Muscle / Q000201:enzymology*; D011247:Pregnancy; D011248:Pregnancy Complications; D011502:Protein-Energy Malnutrition / Q000201:enzymology* / Q000503:physiopathology; D051381:Rats; D017208:Rats, Wistar
D006038:Glyoxylates; C030921:4-hydroxyphenylglyoxylic acid; D008316:Malonyl Coenzyme A; D002334:Carnitine O-Palmitoyltransferase