Effect of caerulein on expression of the immediate-early genes c-fos and zif/268 in the rat brain.
The effect of caerulein, an analog of cholecystokinin-8, on expression of the immediate-early genes c-fos and zif/268 was studied in the rat brain using Northern blot analysis and an in situ hybridization technique. Intraperitoneal injection of caerulein did not change the basal c-fos and zif/268 expression. Administration of the convulsant, pentylenetetrazole (PTZ), caused a dramatic increase of c-fos and zif/268 mRNAs in the hippocampus and dentate gyrus. Pretreatment with caerulein suppressed the PTZ-induced c-fos and zif/268 expression. It is considered that systemically administrated caerulein modifies neuronal activities by exerting a suppressed effect on induction of the immediate-early genes.
Miyoshi
R
R
Department of Pharmacology, Tokyo Women's Medical College, Japan.
Kito
S
S
Muraki
T
T
Weiser
M
M
Joh
T H
TH
D000818:Animals; D001345:Autoradiography; D001483:Base Sequence; D015152:Blotting, Northern; D001923:Brain Chemistry / Q000187:drug effects*; D002108:Ceruletide / Q000494:pharmacology*; D015342:DNA Probes; D005786:Gene Expression Regulation / Q000187:drug effects*; D016762:Genes, fos / Q000187:drug effects*; D006624:Hippocampus / Q000378:metabolism; D017403:In Situ Hybridization; D008297:Male; D008969:Molecular Sequence Data; D010433:Pentylenetetrazole / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar
D015342:DNA Probes; D002108:Ceruletide; D010433:Pentylenetetrazole