title: Inhibitory effects of hyaluronan on [14C]arachidonic acid release from labeled human synovial fibroblasts.
abstract: The effects of hyaluronan (HA) on the release of arachidonic acid (AA) from phospholipids induced by bradykinin in synovial fibroblasts of osteoarthritic patients were examined. HA inhibited [14C]AA release from prelabeled synovial cells stimulated with and without bradykinin 1 hr after incubation with HA and thereafter. The inhibitory effects of HA on [14C]AA release were dependent on the concentration and molecular weight of HA. However, inhibition of [14C]AA release by HA was not merely due to the viscosity of HA. The [14C]AA release induced by calcium-ionophore A23187 was also inhibited by HA with a high molecular weight. In addition, HA did not affect [14C]AA uptake by the cells. Our results suggest that HA with a high molecular weight elicits anti-inflammatory effects, at least in part, by inhibiting AA release in inflamed joints.
authors: Research Laboratories, Maruho Co., Ltd., Osaka, Japan.
authors: K
authors: K
authors: Tobetto
authors: T
authors: T
authors: Yasui
authors: T
authors: T
authors: Ando
authors: M
authors: M
authors: Hayaishi
authors: N
authors: N
authors: Motohashi
authors: M
authors: M
authors: Shinogi
authors: I
authors: I
authors: Mori
mesh_terms: D016718:Arachidonic Acid / Q000378:metabolism*; D001920:Bradykinin / Q000494:pharmacology; D000001:Calcimycin / Q000494:pharmacology; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D006820:Hyaluronic Acid / Q000494:pharmacology*; D050356:Lipid Metabolism; D008747:Methylcellulose / Q000494:pharmacology; D008970:Molecular Weight; D010003:Osteoarthritis / Q000188:drug therapy; D013582:Synovial Fluid / Q000166:cytology / Q000378:metabolism*; D014783:Viscosity
chemical_list: D016718:Arachidonic Acid; D000001:Calcimycin; D006820:Hyaluronic Acid; D008747:Methylcellulose; D001920:Bradykinin