High-performance liquid chromatographic determination of BRB-I-28, a novel antiarrhythmic agent, in dog plasma and urine.
A sensitive reversed-phase high-performance liquid chromatographic (HPLC) technique with ultraviolet detection has been developed to determine the concentration of BRB-I-28 (I), a novel antiarrhythmic agent, in dog plasma and urine. The mobile phase was acetonitrile-methanol-37.5 mM phosphate buffer, pH 6.8-triethylamine (50:50:75:0.1, v/v). The compound was extracted from dog plasma and urine with chloroform after alkalinization with sodium hydroxide. The extraction recovery was 83% from plasma and 84% from urine. Good linearity (r > 0.996) was observed throughout the ranges 0.1-12.0 micrograms/ml (plasma) and 0.1-8.0 micrograms/ml (urine). Intra- and inter-assay variabilities were less than 4%. The lower limit of quantitation was 0.08 microgram/ml in either plasma or urine. HPLC analysis of plasma and urine samples from a dog treated with I has demonstrated that the method was accurate and reproducible.
Chen
C L
CL
Department of Physiological Sciences, Oklahoma State University, Stillwater 74078.
Lesseley
B A
BA
Clarke
C R
CR
Roder
J D
JD
Sangiah
S
S
Berlin
K D
KD
Garrison
G L
GL
Scherlag
B J
BJ
Lazzara
R
R
Patterson
E
E
D000818:Animals; D000889:Anti-Arrhythmia Agents / Q000032:analysis* / Q000097:blood / Q000652:urine; D001643:Bridged Bicyclo Compounds / Q000032:analysis* / Q000097:blood / Q000652:urine; D019086:Bridged Bicyclo Compounds, Heterocyclic; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004285:Dogs; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet
D000889:Anti-Arrhythmia Agents; D001643:Bridged Bicyclo Compounds; D019086:Bridged Bicyclo Compounds, Heterocyclic; C041492:7-benzyl 3-thia-7-azabicyclo(3.3.1)nonane