Determination of nicotinamide and pyridoxine in an elemental diet by column-switching high-performance liquid chromatography with UV detection.
The determination of nicotinamide and pyridoxine in an elemental diet containing 46 compounds was performed by column-switching high-performance liquid chromatography with UV detection at 260 and 290 nm, respectively. The method is simple, rapid, sensitive and reproducible. The calibration graphs for the two vitamins were linear in the ranges 0-0.2 and 0-0.015 micrograms, respectively. The recoveries of both vitamins by the standard addition method were over 95%. There was good agreement between the concentrations indicated and found for both vitamins.
Iwase
H
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Ajinomoto Co., Inc., Kawasaki Factory, Japan.
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005526:Food, Formulated / Q000032:analysis*; D009536:Niacinamide / Q000032:analysis*; D011736:Pyridoxine / Q000032:analysis*; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet
D009536:Niacinamide; D011736:Pyridoxine