[Detection of HBV-DNA in peripheral blood mononuclear cells of anti-HIV-positive patients and its relation to other serological markers of HBV].
HBV infection has been investigated in 47 anti-human HIV positive patients in relation to a similar group of 33 anti-HIV negative patients. Serological HBV markers were found in 87% of anti-HIV positive patients. The difference in markers of viral replication (HBeAg, HBV-DNA) was not statistically significant between the two groups. It has been suggested that HBV infection of peripheral blood mononuclear cells could be a cofactor implicated in the development of immunodeficiency due to HIV. For this reason we have investigated the presence of HBV-DNA in peripheral blood mononuclear cells by in situ hybridization. Although its detection was more frequent in anti-HIV positive patients than in anti-HIV negative ones (p < 0.05), it was not related to clinical state of immunodeficiency. With regard to serological HBV markers, HBV-DNA was detected in peripheral blood mononuclear cells from antiHBc w/o antiHBs patients. This fact means the virus may persist in this cells after recovery and suggest they could serve as additional reservoirs of HBV. These cells, that contain the HBV genome, could be implicated in the perpetuation, reactivation of the infection and in its transmission.
Suñén
E
E
Departamento de Microbiología e Inmunología, Universidad del País Vasco, Hospital Civil de Bilbao.
Malave
C
C
Sarriá
L
L
Gorriño
C
C
Campelo
C
C
Martínez
J
J
Cisterna
R
R
D000328:Adult; D004279:DNA, Viral / Q000097:blood*; D005260:Female; D015483:HIV Antibodies / Q000097:blood; D015658:HIV Infections / Q000150:complications*; D006509:Hepatitis B / Q000150:complications / Q000175:diagnosis* / Q000453:epidemiology / Q000635:transmission; D006510:Hepatitis B Antibodies / Q000097:blood*; D006511:Hepatitis B Antigens / Q000097:blood*; D006515:Hepatitis B virus / Q000276:immunology / Q000302:isolation & purification* / Q000502:physiology; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000382:microbiology*; D008297:Male; D015995:Prevalence; D014775:Virus Activation; D014779:Virus Replication
D004279:DNA, Viral; D015483:HIV Antibodies; D006510:Hepatitis B Antibodies; D006511:Hepatitis B Antigens
Detección del ADN-VHB en células mononucleares de sangre periférica de pacientes anti-VIH-positivos, y su relación con otros marcadores serológicos del VHB.