Allopurinol administered prior to hepatic ischaemia in the rat prevents chemiluminescence following restoration of circulation.
Oxygen-derived free radicals produced during reperfusion may be responsible for the disturbed pathology which follows prolonged ischaemia. Measurement of hepatic chemiluminescence (low level light emission resulting from the energy released during chemical reactions of free radicals) allowed determination of whether allopurinol could prevent formation of oxygen-derived free radicals during reperfusion of the ischaemic liver. While control animals demonstrated a burst of light emission shortly after reperfusion, the rats pretreated with allupurinol showed no evidence of chemiluminescence during either ischaemia or reperfusion. It is concluded that allopurinol may modify reperfusion-induced free radical formation and possibly ameliorate the organ damage which can follow ischaemia.
Cohen
P J
PJ
Department of Anesthesia, University of Pennsylvania Medical School, Philadelphia 19104.
D000284:Administration, Oral; D000493:Allopurinol / Q000008:administration & dosage / Q000494:pharmacology*; D000818:Animals; D005609:Free Radicals / Q000037:antagonists & inhibitors / Q000378:metabolism; D007511:Ischemia / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects*; D008099:Liver / Q000098:blood supply / Q000187:drug effects* / Q000378:metabolism; D008102:Liver Circulation; D008163:Luminescent Measurements; D010100:Oxygen; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000378:metabolism / Q000517:prevention & control*
D005609:Free Radicals; D000493:Allopurinol; D010100:Oxygen