title: Characterization in vitro and in vivo of resistance to ionophores in a strain of Eimeria tenella.
abstract: A field isolate of Eimeria tenella (FS139) was propagated several times in chickens medicated with 200 ppm of dietary monensin. In a laboratory test with 2-wk-old-chickens, the strain was resistant to monensin, salinomycin, and lasalocid given at double use level and was resistant to narasin and maduramicin at the normal use level. In comparison, a laboratory strain (WIS) was controlled by the normal use level of each product. When free WIS sporozoites were treated in vitro with 1.0 microgram/ml of monensin for 0.5 or 4.0 hr at 41 C and inoculated into primary cultures of chicken kidney cells the invasion was reduced by 35.6% or 96.3%, but invasion of FS139 sporozoites was increased by 18.5% by 0.5 hr treatment and was about the same as controls after 2 hr of treatment. Few sporozoites from the WIS strain developed into schizonts, but numerous sporozoites from the FS139 strain developed into normal first and second generation schizonts. The structure of free WIS sporozoites was distorted after 3 hr of treatment with 2.5 micrograms/ml of monensin at 41 C, as observed by light and scanning electron microscopy, whereas there was no change in structure of most treated FS139 sporozoites.
authors: Department of Poultry Science, University of Georgia, Athens 30602.
authors: G
authors: G
authors: Zhu
authors: L R
authors: LR
authors: McDougald
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002645:Chickens / Q000469:parasitology*; D003048:Coccidiosis / Q000188:drug therapy / Q000662:veterinary*; D004351:Drug Resistance; D016786:Eimeria tenella / Q000187:drug effects* / Q000648:ultrastructure; D007476:Ionophores / Q000494:pharmacology* / Q000627:therapeutic use; D008855:Microscopy, Electron, Scanning; D011201:Poultry Diseases / Q000188:drug therapy*; D015430:Weight Gain
chemical_list: D007476:Ionophores