Influence of bile acids on stimulated lipid peroxidation and hydrogen peroxide production in rat liver microsomes.
Bile acids were found to be effective antioxidants in bile and intestine. The influence of different bile acids on the NADPH-Fe(++)-stimulated lipid peroxidation (LPO) and cytochrome P-450 dependent hydrogen peroxide production (H2O2) in rat liver microsomes was investigated in vitro. LPO was determined as production of thiobarbituric acid reactants (TBAR). Different tri-, di- and monohydroxylated bile acids and cholesterol were given to the incubation mixture in concentrations ranging from 10(-5) to 10(-3) M. Sodium salts of cholic, tauroglycocholic and deoxycholic acids as well as cheno-deoxycholic, ursodeoxycholic, lithocholic acids and cholesterol did not alter the microsomal production of TBAR. H2O2 formation was significantly decreased by sodium deoxycholate whereas cholesterol increased H2O2 production up to 4 times. These results show that bile acids were not able to protect microsomal membrane lipids against peroxidative damage. Cholesterol mediated H2O2 formation as a source of hydroxyl radicals had no toxic effect concerning LPO, TBAR were not enhanced significantly.
Barth
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A
Institute of Pharmacology and Toxicology, Friedrich Schiller University Jena, Germany.
Bernst
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M
D000818:Animals; D001647:Bile Acids and Salts / Q000494:pharmacology*; D002251:Carbon Tetrachloride / Q000494:pharmacology; D002784:Cholesterol / Q000494:pharmacology; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D005296:Ferrous Compounds / Q000494:pharmacology; D006861:Hydrogen Peroxide / Q000378:metabolism*; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism*; D009249:NADP / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D013858:Thiobarbiturates
D001647:Bile Acids and Salts; D005296:Ferrous Compounds; D013858:Thiobarbiturates; D009249:NADP; D003577:Cytochrome P-450 Enzyme System; D002784:Cholesterol; D006861:Hydrogen Peroxide; D002251:Carbon Tetrachloride; C029684:thiobarbituric acid