Has negative staining still a place in biomacromolecular electron microscopy?
Transmission electron microscopy of proteins has provided molecular- and in a few cases near-atomic-resolution structural information. In this review, we critically evaluate the potential and the limitations in obtaining molecular resolution, particularly with negatively stained specimens, and put these into perspective with cryomicroscopy of unstained frozen-hydrated and sugar-embedded preparations.
Bremer
A
A
M.E. Müller Institute for High-Resolution Electron Microscopy, University of Basel, Switzerland.
Henn
C
C
Engel
A
A
Baumeister
W
W
Aebi
U
U
D016591:Histocytological Preparation Techniques; D008854:Microscopy, Electron / Q000379:methods* / Q000639:trends; D016624:Negative Staining; D011506:Proteins / Q000648:ultrastructure*
D011506:Proteins