[The inhibitory effects of platelet activating factor (PAF) on ciliary activity of human paranasal sinus mucosa].
The effect of platelet activating factor (PAF) on human paranasal ciliated cells was investigated in vitro. Normal human paranasal sinus mucosa was obtained by surgical procedure and incubated with Eagle's MEM containing 10% FCS in the form of tissue culture. Ciliary activity was viewed at 37 degrees C under an inverted microscope equipped with a thermoregulator and a humidified CO2 chamber, recorded on video tapes and photoelectrically measured. Ciliary inhibition was observed by the treatment with PAF, in a dose dependent manner, at concentrations from 10(-10) M to 10(-6) M. The inhibitory effect of 10(-8) M PAF on ciliary activity was completely blocked when the mucosa was treated with 10(-6) M CV-3988 or 10(-6) M CV-6209 (specific PAF receptor antagonists). By the radioimmunoassay, the concentration of PAF in tissue culture was reduced by half within 12.5 min, and within 60 min it was only 5% of the initial concentration. There was no significant difference in ciliary inhibition between irrigation after a 60 min incubation with 10(-8) M PAF and non-irrigation. These results indicate that PAF inhibited ciliary activity directly and specifically, and induced irreversible damage primarily within the first 60 min after the challenge.
Ganbo
T
T
Department of Otorhinolaryngology, Isawa Kyoutou Hospital.
Hisamatsu
K
K
Inoue
H
H
Horiguchi
S
S
Murakami
Y
Y
D002923:Cilia / Q000187:drug effects; D003864:Depression, Chemical; D004305:Dose-Response Relationship, Drug; D006801:Humans; D066298:In Vitro Techniques; D009092:Mucous Membrane / Q000187:drug effects; D010256:Paranasal Sinuses / Q000187:drug effects*; D010972:Platelet Activating Factor / Q000494:pharmacology*
D010972:Platelet Activating Factor