Structural analysis of the non-dialysable urinary glucoconjugates of normal men.
Enzymic methods were employed to analyse the structure of the non-dialysable urinary glucoconjugates of ten healthy males. The excretion of the urinary glucoconjugates was determined from the glucosyl:galactosyl ratio after acid hydrolysis, and a mean value of 0.27 was obtained. The results of the specific actions of alpha- and beta-glucosidases showed that the non-dialysable urinary glucoconjugates contain a branched alpha-glucan fraction with 1,4- and 1,6-glucosidic bonds, and a beta-glucan fraction containing 1,4-glucosidic bonds.
Adedeji
O O
OO
Nuffield Department of Clinical Biochemistry, John Radcliffe Hospital, Oxford, UK.
D000328:Adult; D002480:Cellulase / Q000378:metabolism; D005087:Glucan 1,4-alpha-Glucosidase / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D006001:Glycoconjugates / Q000652:urine*; D006026:Glycoside Hydrolases / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D008297:Male; D008875:Middle Aged; D010762:Phosphorylase a / Q000378:metabolism; D000520:alpha-Glucosidases / Q000378:metabolism; D001614:beta-Amylase / Q000378:metabolism; D001617:beta-Glucosidase / Q000378:metabolism
D006001:Glycoconjugates; D010762:Phosphorylase a; D006026:Glycoside Hydrolases; D001614:beta-Amylase; D000520:alpha-Glucosidases; D001617:beta-Glucosidase; D005087:Glucan 1,4-alpha-Glucosidase; D002480:Cellulase; C100181:pullulanase; D005947:Glucose