[Pharmacological study on Panax ginseng C. A. Meyer. XV. Effects of 70% methanolic extract from red and white ginseng on the antitumor activity of mitomycin C].
The influence of various fractions and ginsenosides from the 70% methanolic extract (RMe) of Red Ginseng (a steamed and dried root of Panax ginseng C. A. Meyer) on the cytocidal effect of mitomycin C (MMC) against Ehrlich ascites carcinoma was investigated in vitro. The AcOEt soluble portion (RMe-I) showed an increasing effect on the activities of lysosomal enzymes in the cultured tumor cells. RMe-I promoted the uptake of MMC into the tumor cells and enhanced the cytotoxicity of MMC against the cultured tumor cells. 20(S)-, 20(R)-ginsenoside Rg3 and ginsenoside Rh2 isolated from RMe-I promoted the uptake of MMC into the tumor cells but ginsenosides from the n-BuOH soluble portion (RME-II) had no effect. Furthermore, the influence of RMe and the 70% methanolic extract (WMe) from White Ginseng (a dried root of Panax ginseng C. A. Meyer) on the cytocidal effect of MMC was investigated in vivo. MMC combined with RMe showed stronger antitumor effects against the ascites form of mouse Ehrlich ascites carcinoma and rat ascites hepatoma AH 130 than MMC combined with WMe. The activities of lysosomal enzymes in tumor cells were also more increased in comparison with that combined MMC and WMe.
Tong
C N
CN
Faculty of Pharmaceutical Sciences, Kinki University, Osaka, Japan.
Matsuda
H
H
Kubo
M
M
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000378:metabolism / Q000473:pathology*; D004354:Drug Screening Assays, Antitumor; D004357:Drug Synergism; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D036145:Ginsenosides; D008114:Liver Neoplasms, Experimental / Q000378:metabolism / Q000473:pathology*; D000432:Methanol; D051379:Mice; D016685:Mitomycin / Q000493:pharmacokinetics / Q000494:pharmacology*; D005894:Panax; D010946:Plants, Medicinal; D051381:Rats; D012503:Saponins / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D004365:Drugs, Chinese Herbal; D036145:Ginsenosides; D012503:Saponins; D016685:Mitomycin; D000432:Methanol