YAC-assisted cloning of a putative G-protein mapping to the MHC class I region.
We report the successful use of whole yeast artificial chromosomes (YACs) as probes for direct positional cloning of novel expressed sequences in a given genomic fragment. The class I region of the human major histocompatibility complex, in particular the chromosomal fragment spanning the HLA-E locus, was investigated. The screening of a cDNA library with a 210-kb-long YAC clone led to the identification of a new gene, positionally conserved in the major histocompatibility complex of the mouse genome and encoding a putative GTP binding protein. Although its precise function remains unknown, the interspecies conservation of both sequence and map position suggests a regulatory or functional link with the histocompatibility cluster.
Denizot
F
F
Centre d'Immunologie, INSERM-CNRS de Marseille-Luminy, France.
Mattei
M G
MG
Vernet
C
C
Pontarotti
P
P
Chimini
G
G
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D015825:Chromosomes, Fungal; D003001:Cloning, Molecular; D004247:DNA; D019204:GTP-Binding Proteins / Q000235:genetics*; D005805:Genes, MHC Class I; D015894:Genome, Human; D015698:Genomic Library; D006680:HLA Antigens / Q000235:genetics; D015395:Histocompatibility Antigens Class I / Q000235:genetics; D006801:Humans; D017403:In Situ Hybridization; D007621:Karyotyping; D051379:Mice; D008969:Molecular Sequence Data; D005810:Multigene Family; D016415:Sequence Alignment; D014407:Tumor Cells, Cultured; D000097625:HLA-E Antigens
D004247:DNA; D019204:GTP-Binding Proteins; D006680:HLA Antigens; D015395:Histocompatibility Antigens Class I; D000097625:HLA-E Antigens