Determination of human mast cell tryptase by bioluminescence-enhanced two-site immunometric assay.
A highly sensitive bioluminescence-enhanced two-site immunometric assay for human mast cell tryptase was developed. The usable range of the standard curve was from 10 ng to 10 micrograms per litre. The intra-assay coefficient of variation of the test was between 8.0 and 12.3%, and the inter-assay coefficient of variation between 8.3 and 13.5%. Recovery of human mast cell tryptase in human serum was 100% (fraction of serum in the test sample: 0.30).
Schneider
E
E
Medor GmbH, Herrsching, Bundesrepublik Deutschland.
Gabrijelcic
D
D
Geiger
R
R
D002021:Buffers; D053818:Chymases; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D008163:Luminescent Measurements; D008407:Mast Cells / Q000201:enzymology*; D012697:Serine Endopeptidases / Q000378:metabolism*; D053802:Tryptases
D002021:Buffers; D012697:Serine Endopeptidases; C083294:chymase 2; D053818:Chymases; D053802:Tryptases