Use of charged tails in protein-purification fusions. Polyaspartic acid tails in Aspergillus glucoamylase expressed in yeast.
Suominen
I
I
Department of Biochemistry, University of Turku, Finland.
Nurmela
H
H
Heimo
H
H
Ford
C
C
Stachon
D
D
Glatz
C
C
D000595:Amino Acid Sequence; D001224:Aspartic Acid; D001230:Aspergillus / Q000201:enzymology; D001483:Base Sequence; D011232:Chemical Precipitation; D003001:Cloning, Molecular; D005656:Fungal Proteins / Q000302:isolation & purification; D005087:Glucan 1,4-alpha-Glucosidase / Q000302:isolation & purification*; D007526:Isoelectric Point; D008969:Molecular Sequence Data; D010957:Plasmids; D015202:Protein Engineering / Q000379:methods*; D011993:Recombinant Fusion Proteins / Q000302:isolation & purification*; D012441:Saccharomyces cerevisiae
D005656:Fungal Proteins; D011993:Recombinant Fusion Proteins; D001224:Aspartic Acid; D005087:Glucan 1,4-alpha-Glucosidase