An idiotypic-anti-idiotypic competitive immunoassay for quantitation of okadaic acid.
A competitive indirect enzyme-linked immunosorbent assay for the measurement of okadaic acid, a marine toxin, was developed. The assay uses a murine monoclonal anti-idiotypic antibody bearing an internal image of okadiac acid epitope to capture an anti-okadaic acid monoclonal antibody in the presence of free okadaic acid. Bound anti-okadaic acid antibody is detected with peroxidase-conjugated anti-mouse immunoglobulin antiserum. If present, free toxin will lessen the amount of anti-okadaic acid antibody binding to its corresponding anti-idiotypic antibody in a dose-dependent manner that can be quantified from the standard curve. The assay permits reliable measurement of okadaic acid in the 9-81 ng/ml range. The intra- and interassay coefficients of variation in the measurement of OA in the toxin spiked mussel samples averaged 9% and 12%, respectively. The assay is rapid, accurate, reproducible and relatively simple to perform. It may be of potential use to laboratories involved in monitoring the toxin levels in plankton, seafood or sponges.
Shestowsky
W S
WS
Rougier Bio-Tech Ltd, Montreal, Quebec, Canada.
Quilliam
M A
MA
Sikorska
H M
HM
D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D049872:Bivalvia / Q000737:chemistry; D002273:Carcinogens / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D004797:Enzyme-Linked Immunosorbent Assay; D004988:Ethers, Cyclic / Q000032:analysis* / Q000276:immunology; D007118:Immunoassay; D019319:Okadaic Acid; D012758:Shellfish / Q000032:analysis
D000911:Antibodies, Monoclonal; D002273:Carcinogens; D004988:Ethers, Cyclic; D019319:Okadaic Acid