Intracellular and serum levels of aldolase activity in B chronic lymphocytic leukemia.
Intracellular and serum activities of aldolase (ALS) were biochemically determined in lymphocyte subpopulations from normal subjects and patients with B-chronic lymphocytic leukemia (B-CLL). Aldolase activity was significantly lower in T cells of CLL than in normal T cells (2.9 +/- 1.5 vs. 4.7 +/- 2.1 Sigma Units (SU)/6 x 10(6) cells, p < 0.05). The aldolase activity also was significantly (p < 0.001) lower (3.1 +/- 1.9 SU/6 x 10(6) cells) in CLL B lymphocytes than in normal B lymphocytes (18.1 +/- 6.5 SU/6 x 10(6) cells). Moreover, the serum levels of ALS in all patients with B-CLL were higher than that in normal subjects (8.1 +/- 5.8 vs. 2.2 +/- 0.8 SU/ml, p < 0.02). Our findings demonstrate that T lymphocytes from patients with B-CLL display enzyme activity different from that of normal T cells. This may reflect the abnormal maturity of the residual T cell population in B-CLL.
Musolino
C
C
Institute of Internal Medicine, Messina University, Italy.
Alonci
A
A
Allegra
A
A
Di Cesare
E
E
Orlando
A
A
Grosso
P
P
Buda
G
G
Squadrito
G
G
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015703:Antigens, CD / Q000032:analysis; D000951:Antigens, Neoplasm / Q000032:analysis; D016175:B-Lymphocyte Subsets / Q000737:chemistry / Q000201:enzymology; D014408:Biomarkers, Tumor / Q000032:analysis*; D005260:Female; D005634:Fructose-Bisphosphate Aldolase / Q000032:analysis*; D006801:Humans; D016130:Immunophenotyping; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000201:enzymology*; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000032:analysis*; D014411:Neoplastic Stem Cells / Q000737:chemistry / Q000201:enzymology; D016176:T-Lymphocyte Subsets / Q000737:chemistry / Q000201:enzymology
D015703:Antigens, CD; D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins; D005634:Fructose-Bisphosphate Aldolase