Nucleolar and cytoplasmic localization of annexin V.
The subcellular localization of annexin V in cultured human umbilical vein endothelial cells, epithelial cells and fibroblasts was examined. Indirect immunofluorescence and immunoblotting studies using affinity-purified anti-annexin V antibodies revealed that annexin V is located within the cytoplasm and nucleus of these cells. Further examination and direct binding studies showed that annexin V within the nucleus is associated with the nucleolus. These findings suggest that annexin V may play a role in a nucleolar function, such as ribosome assembly and transport.
Sun
J
J
Department of Medicine, Monash Medical School, Box Hill Hospital, Australia.
Salem
H H
HH
Bird
P
P
D017304:Annexin A5 / Q000032:analysis* / Q000276:immunology / Q000378:metabolism; D000918:Antibody Specificity; D002460:Cell Line; D002466:Cell Nucleolus / Q000737:chemistry*; D002478:Cells, Cultured; D003593:Cytoplasm / Q000737:chemistry*; D004730:Endothelium, Vascular / Q000737:chemistry / Q000166:cytology; D005455:Fluorescent Antibody Technique; D006367:HeLa Cells; D006801:Humans
D017304:Annexin A5