Isolation and structures of microcystins from a cyanobacterial water bloom (Finland).
A hepatotoxic cyanobacterial (blue-green algal) water bloom was collected from a constructed water reservoir in Finland. The water bloom contained two cyanobacterial species, Microcystis aeruginosa and Aphanizomenon flos-aquae. Two hepatotoxins, 1 and 2, were isolated from extracts of lyophilized cells. The structures of 1 and 2 were assigned based upon their amino acid analyses on a Waters Pico Tag HPLC system and a chiral GC capillary column (Chirasil Val III), fast atom bombardment mass spectrometry (FABMS), high resolution FABMS, and tandem FABMS data. Toxin 1 was identical to a previously reported compound, [D-Asp3]microcystin-RR. Toxin 2 was new and was assigned the structure [D-Asp3]microcystin-YR.
Namikoshi
M
M
School of Chemical Sciences, University of Illinois, Urbana 61801.
Sivonen
K
K
Evans
W R
WR
Sun
F
F
Carmichael
W W
WW
Rinehart
K L
KL
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D000458:Cyanobacteria / Q000737:chemistry*; D006868:Hydrolysis; D008297:Male; D008387:Marine Toxins; D051379:Mice; D008813:Mice, Inbred ICR; D052998:Microcystins; D010456:Peptides, Cyclic / Q000032:analysis / Q000302:isolation & purification*; D016339:Spectrometry, Mass, Fast Atom Bombardment
D000596:Amino Acids; D008387:Marine Toxins; D052998:Microcystins; D010456:Peptides, Cyclic; C065685:microcystin YR; C063855:microcystin RR