title: The development of neuropeptide Y-immunoreactive neurons in a model of pure cortical culture.
abstract: The development of neuropeptide Y-immunoreactive neurons in the rat brain cerebral cortex was studied in a model of a pure cortical culture. In this model, development of neurons devoid of any afferents from other brain structures could be observed. Since mutual interactions between neuropeptide Y and catecholamines have been postulated, such a pure cortical culture offers a possibility of studying the development of neuropeptide Y neurons devoid of any brainstem monoaminergic afferents. A tissue dissected from 16-day-old rat fetuses and cultivated in a dissociated culture for 14 days was examined immunohistochemically for the presence of neuropeptide Y-immunoreactive neurons. Three main types of neuropeptide Y-immunoreactive neurons were found: unipolar, bipolar and multipolar. Cell processes and terminal varicose fibres were also observed. The results obtained indicate that neuropeptide Y-immunoreactive neurons and fibres may develop in a pure culture of the rat cerebral cortex without the influence of any other structures.
authors: Laboratory of Neurobiology, Polish Academy of Sciences, Kraków.
authors: M
authors: M
authors: Smiałowska
authors: B
authors: B
authors: Legutko
mesh_terms: D000818:Animals; D001933:Brain Stem / Q000166:cytology / Q000502:physiology; D002395:Catecholamines / Q000378:metabolism / Q000502:physiology; D002540:Cerebral Cortex / Q000033:anatomy & histology / Q000166:cytology / Q000502:physiology*; D005260:Female; D007150:Immunohistochemistry; D009474:Neurons / Q000502:physiology*; D009475:Neurons, Afferent / Q000378:metabolism / Q000502:physiology; D009478:Neuropeptide Y / Q000276:immunology / Q000502:physiology*; D011247:Pregnancy; D051381:Rats; D017208:Rats, Wistar
chemical_list: D002395:Catecholamines; D009478:Neuropeptide Y