Does Lp(a) lipoprotein inhibit the fibrinolytic system?
Lp(a) lipoprotein contains a unique apolipoprotein, apolipoprotein (a), that has a striking homology with plasminogen. This homology has brought forward speculations as to an inhibitory effect of Lp(a) lipoproteins on fibrinolysis. The present investigation was undertaken to study the influence of Lp(a) lipoprotein on the fibrinolytic system. In an in vitro model, we have studied the influence of purified Lp(a) lipoprotein on plasminogen activation by tissue plasminogen activator (t-PA) in the presence of soluble fibrin. Increasing concentrations of Lp(a) lipoprotein (0-32 mg/dl) did not inhibit plasminogen activation by t-PA in the presence of thrombin or bathroxobin digested fibrinogen. When purified Lp(a) lipoprotein was added to whole blood, the degree of fibrin degradation obtained following standardized coagulation, as evaluated by the generation of D-dimer, was not reduced. D-dimer levels in plasma and in serum after standardized coagulation, as well as conventional parameters for evaluation of the fibrinolytic system, were determined in 10 individuals with high and 10 individuals with low levels of Lp(a) lipoprotein. No differences in the fibrinolytic parameters were observed between the groups. Thus, we found no evidence that Lp(a) lipoprotein interferes with the fibrinolytic process in the present experiments.
Halvorsen
S
S
Haematological Research Laboratory, Ullevaal Hospital, Oslo, Norway.
Skjønsberg
O H
OH
Berg
K
K
Ruyter
R
R
Godal
H C
HC
D000595:Amino Acid Sequence; D005260:Female; D005337:Fibrin / Q000378:metabolism; D005338:Fibrin Fibrinogen Degradation Products / Q000378:metabolism; D005342:Fibrinolysis / Q000187:drug effects*; D006801:Humans; D066298:In Vitro Techniques; D008055:Lipids / Q000097:blood; D017270:Lipoprotein(a) / Q000302:isolation & purification / Q000494:pharmacology*; D008297:Male; D008969:Molecular Sequence Data; D012995:Solubility; D010959:Tissue Plasminogen Activator / Q000494:pharmacology
D005338:Fibrin Fibrinogen Degradation Products; D008055:Lipids; D017270:Lipoprotein(a); C036309:fibrin fragment D; D005337:Fibrin; D010959:Tissue Plasminogen Activator