Expression of human salivary-gland kallikrein in insect cells by a baculovirus vector.
A cDNA fragment encoding human salivary-gland kallikrein, including the kallikrein-owned signal peptide, was inserted into a baculovirus vector adjacent to the polyhedrin promoter and expressed in transfected insect cells. Biologically active kallikrein was isolated to homogeneity from serum-free culture supernatant using a four-step protocol. The N-terminal amino acid sequence of the insect-derived kallikrein was identical to that of the natural proteinase, thus indicating the proper removal of the mammalian signal peptide.
Rahn
H P
HP
Institut für Biochemie, Technische Hochschule Darmstadt, FRG.
Angermann
A
A
Hektor
T
T
Klöppinger
M
M
Kemme
M
M
D000595:Amino Acid Sequence; D000818:Animals; D016367:Baculoviridae / Q000235:genetics; D002460:Cell Line; D015870:Gene Expression; D005822:Genetic Vectors; D007313:Insecta; D007610:Kallikreins / Q000235:genetics* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification; D012469:Salivary Glands / Q000378:metabolism*
D011994:Recombinant Proteins; D007610:Kallikreins