Comparison between histochemical and immunohistochemical methods for diagnosis of sporotrichosis.
OBJECTIVE
To compare the efficacy of histochemical and immunohistochemical methods in detecting forms of Sporothrix schenckii in tissue.


METHODS
Thirty five cutaneous biopsy specimens from 27 patients with sporotrichosis were stained by histochemical haematoxylin and eosin, periodic acid Schiff, and Gomori's methenamine silver methods and an immunohistochemical (avidin-biotin complex immunoperoxidase) (ABC) technique associated with a newly produced rabbit polyclonal antibody anti-Sporothrix schenckii.


RESULTS
A total of 29 (83%) cases were positive by the ABC method used in association with anti-Sporothrix schenckii rabbit polyclonal antibodies. Histochemical methods, using silver staining, periodic acid Schiff, and conventional haematoxylin and eosin detected 37%, 23%, and 23% of forms of S schenckii, respectively. The ABC technique was significantly more reliable than periodic acid Schiff and silver staining techniques.


CONCLUSIONS
It is concluded that immunostaining is an easy and rapid method which can efficiently increase the accuracy of the diagnosis of sporotrichosis in human tissue.
Marques
M E
ME
Department of Pathology, Botucatu Medical School-UNESP, Botucatu, São Paulo, Brazil.
Coelho
K I
KI
Sotto
M N
MN
Bacchi
C E
CE
D000908:Antibodies, Fungal / Q000032:analysis; D006651:Histocytochemistry / Q000379:methods; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D012680:Sensitivity and Specificity; D012867:Skin / Q000276:immunology; D013175:Sporothrix / Q000276:immunology / Q000378:metabolism; D013174:Sporotrichosis / Q000175:diagnosis* / Q000378:metabolism
D000908:Antibodies, Fungal