Monitoring of haemoglobin-methyl isocyanate adduct by high-performance liquid chromatography with diode array detector.
Carbamylation of haemoglobin by methyl isocyanate (MIC) was detected by high-performance liquid chromatography (HPLC) using a photodiode array detector following cyclisation of the N-terminal valine into methyl isopropyl hydantoin (MIH). MIH was also synthesised by reaction of MIC with valine, the chromatographic conditions standardised and the spectrum derived by a photodiode array detector recorded for confirmation of the identity of MIH. This HPLC method is specific, sensitive and suitable for the detection of exposure of blood samples to methyl isocyanate.
Venkateswaran
K S
KS
Defence Research and Development Establishment, Gwalior, India.
Raghuveeran
C D
CD
Gopalan
N
N
Agarwal
G S
GS
Kaushik
M P
MP
Vijayaraghavan
R
R
D000059:Accidents; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003485:Cyanates / Q000097:blood* / Q000506:poisoning; D006454:Hemoglobins / Q000032:analysis / Q000378:metabolism*; D006801:Humans; D007194:India; D017953:Isocyanates; D051381:Rats
D003485:Cyanates; D006454:Hemoglobins; D017953:Isocyanates; C008461:methyl isocyanate