Determination of cocaethylene, cocaine and their metabolites in rat serum microsamples by high-performance liquid chromatography, and its application to pharmacokinetic studies in rodents.
A single-solvent extraction step high-performance liquid chromatographic method is described for quantitating cocaethylene in rat serum microsamples (50 microliters), a substance formed in vivo when cocaine and ethanol are present concurrently. The separation used a 2 mm I.D. reversed-phase Nova-Pak C18 column with a mobile phase of acetonitrile-phosphate buffer containing an ion-pairing reagent. With an ultraviolet detector operated at 230 nm, a linear response was observed from 0.05 to 2.0 micrograms/ml with a detection limit of 5 ng/ml for cocaethylene, cocaine and norcocaine. The method showed a longer half-life for cocaethylene than for cocaine in rat.
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Department of Psychology, Rutgers University, New Brunswick, NJ 08903.
D000284:Administration, Oral; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003042:Cocaine / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000097:blood* / Q000493:pharmacokinetics; D000431:Ethanol / Q000008:administration & dosage; D007274:Injections, Intraperitoneal; D008297:Male; D014179:Neurotransmitter Uptake Inhibitors / Q000097:blood* / Q000493:pharmacokinetics; D051381:Rats
D014179:Neurotransmitter Uptake Inhibitors; D000431:Ethanol; C008671:norcocaine; C066444:cocaethylene; D003042:Cocaine