title: A submucosal mechanism of action for prostaglandin E2 on hexose absorption and metabolism in mouse intestine.
abstract: 1. The involvement of prostaglandin E2 (PGE2) in hexose absorption and metabolism was studied in mouse small intestinal villus cells. 2. Phlorizin-sensitive, Na(+)-dependent alpha-methyl-D-glucoside (alpha-MG) uptake (0.8 mM) during 2 min cell incubations (37 degrees C) was 74 +/- 4 nmol (mg protein)-1. Maximal uptake was 110 +/- 8 nmol (mg protein)-1, representing an accumulation of 50-fold. Metabolism of D-glucose (5 mM) to L-lactate was 38 nmol min-1 (mg protein)-1. 3. Incubation of isolated cells with indomethacin or PGE2 did not affect alpha-MG uptake or D-glucose metabolism. By including indomethacin during cell isolation from whole intestine, alpha-MG uptake was inhibited dose dependently (50-250 microM) by up to 70% (P < 0.001). PGE2 present during both isolation and incubation inhibited by 85% (P < 0.001) at 1 microM and by 27% (P < 0.05) at 0.1 microM, with no effect at lower concentrations. alpha-MG uptake was reduced to 38% (P < 0.01) when 1 microM-PGE2 and 250 microM-indomethacin were presented in combination. When present during cell isolation and incubation, 1 microM-PGE2 inhibited lactate production by 24% (P < 0.05), except when present in combination with 250 microM-indomethacin. Indomethacin, itself, had no effect on lactate production. 4. A submucosal mechanism is proposed to account for the observed inhibitory effects of PGE2 on brush-border uptake of alpha-MG and cellular lactate production. Indomethacin appears to exert not only an effect of its own, possibly via PG-independent actions within the submucosa, but at high concentrations also disrupts the effects of exogenously applied PGE2.
authors: Department of Biology, University of York, Heslington.
authors: J A
authors: JA
authors: Dempster
authors: G L
authors: GL
authors: Kellett
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D015232:Dinoprostone / Q000502:physiology*; D005947:Glucose / Q000378:metabolism; D006601:Hexoses / Q000378:metabolism*; D007408:Intestinal Absorption / Q000502:physiology*; D007421:Intestine, Small / Q000378:metabolism*; D007773:Lactates / Q000096:biosynthesis; D019344:Lactic Acid; D008297:Male; D008757:Methylglucosides / Q000493:pharmacokinetics; D051379:Mice; D008807:Mice, Inbred BALB C
chemical_list: D006601:Hexoses; D007773:Lactates; D008757:Methylglucosides; D019344:Lactic Acid; C027020:methylglucoside; D005947:Glucose; D015232:Dinoprostone