Improved production of pentostatin and identification of fermentation cometabolites.
A practical process is described for the large-scale isolation of pentostatin, an adenosine deaminase inhibitor used clinically for the treatment of interferon-refractory hairy cell leukemia. The identities of minor components in the fermentation beer, including 2'-deoxyguanosine, are also reported.
Showalter
H D
HD
Parke-Davis Pharmaceutical Research Division, Warner-Lambert Co., Ann Arbor, Michigan 48105.
Bunge
R H
RH
French
J C
JC
Hurley
T R
TR
Leeds
R L
RL
Leja
B
B
McDonnell
P D
PD
Edmunds
C R
CR
D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D003849:Deoxyguanosine / Q000737:chemistry; D005285:Fermentation; D009682:Magnetic Resonance Spectroscopy; D015649:Pentostatin / Q000737:chemistry* / Q000302:isolation & purification*; D013303:Streptomyces antibioticus / Q000737:chemistry*
D015649:Pentostatin; D003849:Deoxyguanosine