Alteration of cytochrome P-450 2C11-dependent testosterone metabolism in Gunn rat liver.
Cytochrome P-450 2C11 (CYP2C11) is the main isozyme present in adult male rat liver and specifically hydroxylates testosterone in positions 2 alpha and 16 alpha. In Gunn rats, this isozyme is recognized by the anti-CYP2C11 antibody but its activity towards testosterone is dramatically decreased. Moreover, peptide mapping, after digestion of microsomal fractions by V8 protease and probing with anti-CYP2C11 antibody, exhibit a pattern which differs from that obtained with Wistar rats. Taken together, data suggest that the protein sequence of CYP2C11 from the Gunn rat differs from that of the Wistar rat.
Célier
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INSERM U75, Paris, France.
Columelli
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Cresteil
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D000818:Animals; D000906:Antibodies; D001189:Aryl Hydrocarbon Hydroxylases; D003577:Cytochrome P-450 Enzyme System / Q000276:immunology / Q000378:metabolism*; D010450:Endopeptidases; D008099:Liver / Q000201:enzymology*; D008297:Male; D010449:Peptide Mapping; D051381:Rats; D011911:Rats, Gunn; D017208:Rats, Wistar; D013250:Steroid Hydroxylases / Q000378:metabolism*
D000906:Antibodies; D003577:Cytochrome P-450 Enzyme System; D013250:Steroid Hydroxylases; D001189:Aryl Hydrocarbon Hydroxylases; C022724:testosterone 7-alpha-hydroxylase, hamster; D010450:Endopeptidases