High-performance liquid chromatographic determination of nicotinamide and its metabolites in human and murine plasma and urine.
A high-performance liquid chromatographic method is described which enables the determination of nicotinamide and eight of its possible metabolites in human and murine plasma and urine, using ion-pairing on a base-deactivated reversed-phase column. Calibration curves were linear up to 2 mumol/ml for nicotinamide and 200 nmol/ml for the metabolites; both the intra- and inter-assay relative standard deviations ranged between 1 and 8%. In murine plasma, the N-oxide was the major nicotinamide metabolite, but in man, formation of 1-methylnicotinamide and the 2- and 4-pyridones was also significant. In urine, nicotinuric acid was seen in the mouse, but no nicotinic acid metabolites were seen in man.
Stratford
M R
MR
Gray Laboratory of the Cancer Research Campaign, Mount Vernon Hospital, Northwood, Middlesex, UK.
Dennis
M F
MF
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D051379:Mice; D009536:Niacinamide / Q000031:analogs & derivatives / Q000097:blood* / Q000652:urine*; D010087:Oxides / Q000097:blood* / Q000652:urine*; D011725:Pyridines / Q000097:blood / Q000652:urine
D010087:Oxides; D011725:Pyridines; D009536:Niacinamide; C024058:N(1)-methylnicotinamide