Intracellular free [Ca2+] and [Na+] in response to capsaicin in cultured dorsal root ganglion cells.
Intracellular free Ca2+ and Na+ concentrations in cultured rat dorsal root ganglion cells were studied using the intracellular fluorescent probes fura-2 and SBFI, respectively. Capsaicin increased both intracellular [Ca2+] and [Na+]. A rise in cytosolic free Ca2+ could still be seen if Na+ was removed from the outer medium. The results suggest that capsaicin opens up ion channels which are permeable both to Ca2+ and Na+.
Akerman
K E
KE
Department of Biochemistry and Pharmacy, Abo Akademi University, Turku, Finland.
Grönblad
M
M
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002211:Capsaicin / Q000494:pharmacology*; D002478:Cells, Cultured; D016257:Fura-2; D005727:Ganglia, Spinal / Q000187:drug effects / Q000378:metabolism*; D009475:Neurons, Afferent / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D012964:Sodium / Q000378:metabolism*
D012964:Sodium; D002211:Capsaicin; D002118:Calcium; D016257:Fura-2