title: Production of lipid hydroperoxides and depletion of reduced glutathione in liver mitochondria after acute ethanol administration to rats.
abstract: It has been found that acute ethanol (EtOH) intoxication to rats caused approximately 40% depletion of mitochondrial reduced glutathione (GSH). A GSH reduction of similar extent was also observed after the administration to rats of buthionine sulfoximine (BSO), a specific inhibitor of GSH synthesis. The combined treatment of EtOH plus BSO induced a further mitochondrial GSH decrease up to 70% with respect to control. The presence of lipid hydroperoxides in the mitochondrial membrane was observed whenever an additional oxidative stress was associated to a condition of GSH depletion as in the case of EtOH or EtOH plus BSO. Under these conditions a severe derangement in mitochondrial oxidative functions occurred.
authors: Clinical Pharmacology and Toxicology Institute, University of Modena, Italy.
authors: T
authors: T
authors: Trenti
authors: E
authors: E
authors: Sternieri
authors: D
authors: D
authors: Ceccarelli
authors: D
authors: D
authors: Gallesi
authors: A
authors: A
authors: Masini
mesh_terms: D000818:Animals; D019328:Buthionine Sulfoximine; D000431:Ethanol / Q000633:toxicity*; D005260:Female; D005978:Glutathione / Q000378:metabolism*; D008054:Lipid Peroxides / Q000378:metabolism*; D008717:Methionine Sulfoximine / Q000031:analogs & derivatives / Q000494:pharmacology; D008930:Mitochondria, Liver / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D008054:Lipid Peroxides; D008717:Methionine Sulfoximine; D000431:Ethanol; D019328:Buthionine Sulfoximine; D005978:Glutathione