Analysis of human T-cell receptor alpha-chain cDNAs isolated from peripheral blood mononuclear cells.
T-cell receptor alpha-chain cDNA were generated from unstimulated peripheral blood mononuclear cells of a DR2,3,52a individual using a modified anchor PCR method. Fifty-six cDNA clones were identified representing 47 distinct T-cell receptor clonotypes and 26 VA loci. This analysis identified a new VA gene family VA30, and a new member of the VA6 gene family.
Yassai
M
M
Immunogenetics Research Section, Blood Center of Southeastern Wisconsin, Milwaukee 53233.
Bull
M
M
Gorski
J
J
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA; D005260:Female; D006801:Humans; D007963:Leukocytes, Mononuclear; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics*; D013601:T-Lymphocytes / Q000378:metabolism
D016693:Receptors, Antigen, T-Cell, alpha-beta; D004247:DNA