Kallikrein-like activity in nonpregnant and pregnant rat uterus, fetal membranes, placenta and amniotic fluid.
SBTI-resistant kininogenase activity was found in nonpregnant and pregnant rat uterus, placenta, amniotic fluid and fetal membranes. After trypsin treatment the kininogenase activity increased 2-3 fold. Total kininogenase (active plus inactive) was completely blocked by kallikrein antibodies. The physiological role of these kallikrein-like enzymes is unknown. It is speculated that these enzymes play a local role, perhaps in the processing of polypeptide hormones of through the release of kinins in the regulation of uterine blood flow.
Miatello
R M
RM
Laboratory of Experimental Hypertension and Vasoactive Peptides, Argentine Council of Research (CONICET). Mendoza.
Lama
M C
MC
González
E S
ES
Nolly
H L
HL
D000590:Amino Acid Chloromethyl Ketones / Q000737:chemistry / Q000494:pharmacology; D000595:Amino Acid Sequence; D000653:Amniotic Fluid / Q000201:enzymology; D000818:Animals; D002850:Chromatography, Gel; D004789:Enzyme Activation / Q000187:drug effects; D005321:Extraembryonic Membranes / Q000201:enzymology*; D005260:Female; D066298:In Vitro Techniques; D007610:Kallikreins / Q000037:antagonists & inhibitors / Q000276:immunology / Q000378:metabolism*; D008969:Molecular Sequence Data; D010920:Placenta / Q000201:enzymology*; D011247:Pregnancy; D051381:Rats; D017208:Rats, Wistar; D014357:Trypsin / Q000494:pharmacology; D014599:Uterus / Q000201:enzymology*
D000590:Amino Acid Chloromethyl Ketones; C027587:phenylalanyl-phenylalanyl-arginine chloromethyl ketone; D007610:Kallikreins; D014357:Trypsin