Activation of mouse oocytes, fertilization and development of mouse embryos in vitro after staining with trypan blue or fluorescein diacetate.
The influence of vital staining with trypan blue or fluorescein diacetate on the fertilization of mouse oocytes and the developmental potential of mouse embryos was assessed. Neither stain induced spontaneous activation in mouse oocytes, nor did they impair the in vitro development and implantation of mouse zygotes, two-cell embryos, stressed morulae or blastocysts. However, fertilization and subsequent development of mouse oocytes have been shown to be reduced by vital staining.
Nijs
M
M
Center for Reproductive Medicine, Free University of Brussels, Belgium.
Van Blerk
M
M
Van Steirteghem
A C
AC
D000818:Animals; D001757:Blastomeres / Q000187:drug effects; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D005314:Embryonic and Fetal Development / Q000187:drug effects*; D005260:Female; D005306:Fertilization / Q000187:drug effects*; D005307:Fertilization in Vitro; D005452:Fluoresceins / Q000493:pharmacokinetics / Q000494:pharmacology*; D006736:Horses; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D009028:Morula / Q000187:drug effects; D009865:Oocytes / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D006080:Ovarian Follicle / Q000166:cytology; D014343:Trypan Blue / Q000494:pharmacology*; D015053:Zygote / Q000187:drug effects / Q000378:metabolism
D006063:Chorionic Gonadotropin; D005452:Fluoresceins; D014343:Trypan Blue; C018506:diacetylfluorescein