Improved flow cytometric method for HLA-B27 typing.
HLA-B27 is a cell marker of clinical interest because of its high association with certain diseases. The HLA-B27 antigen was detected on lymphocytes using a monoclonal antibody in an indirect immuno-fluorescence assay using a fluorescence flow cytometer. The considerable crossreaction of the monoclonal antibody with the HLA-B7 antigen was effectively suppressed by masking it by means of human anti-HLA-B7 antiserum. The flow cytometric method was evaluated by comparing the results with those obtained by the standard lymphocytotoxicity test and showed complete agreement in 107 selected patient samples.
Janssen
W C
WC
Department of Clinical Laboratories, Catharina Hospital, Eindhoven, The Netherlands.
Rouwen
J A
JA
Hoffmann
J J
JJ
D003429:Cross Reactions; D005434:Flow Cytometry / Q000379:methods; D005455:Fluorescent Antibody Technique; D015796:HLA-B27 Antigen / Q000032:analysis*; D015793:HLA-B7 Antigen / Q000032:analysis; D006801:Humans; D010641:Phenotype
D015796:HLA-B27 Antigen; D015793:HLA-B7 Antigen