Clastogenic and aneuploidogenic effects of cigarette smoke condensate, mitomycin C and vincristine sulfate.
Immunofluorescent staining of kinetochores in micronuclei (MN) of cytochalasin-B-blocked cells was used to distinguish between clastogenic and aneuploidogenic effects of cigarette smoke condensate (CSC), mitomycin C (MMC) and vincristine sulfate (VS) in Chinese hamster lung V79 cells by using an anti-kinetochore antibody. Within the base-line micronucleated cells (2%), 50 to 58% contained kinetochore-positive (KC+) MN. MMC induced a significantly higher number of MN compared to the controls (P < 0.01), and the majority of micronucleated cells (77% at 0.08 microgram/ml) contained kinetochore-negative (KC-)MN. In contrast, VS induced a large number of MN (P < 0.01) but most of the micronucleated cells contained KC+ MN (93% at 0.08 microgram/ml). Cigarette smoke condensate (CSC) induced both KC+ (P < 0.01) and KC-(P < 0.05) MN suggesting the presence of both clastogenic and aneuploidogenic agents. However, the higher frequency of KC+ than KC- micronucleated cells suggests a stronger aneuploidogenic effect of CSC.
Channarayappa
Division of Plant and Soil Sciences, College of Agriculture and Forestry, West Virginia University, Morgantown 26506-6108.
Nath
J
J
Ong
T
T
D000782:Aneuploidy; D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D015162:Micronucleus Tests / Q000379:methods; D016685:Mitomycin / Q000494:pharmacology; D009153:Mutagens / Q000494:pharmacology*; D010947:Plants, Toxic; D012906:Smoke / Q000009:adverse effects*; D014026:Nicotiana; D014750:Vincristine / Q000494:pharmacology
D009153:Mutagens; D012906:Smoke; D016685:Mitomycin; D014750:Vincristine