A simple method for the isolation of zymosterol from a sterol mutant of Saccharomyces cerevisiae.
A simple method is described for the direct isolation of zymosterol (5 alpha-cholesta-8,24-dien-3 beta-ol) of high purity from a sterol mutant of Saccharomyces cerevisiae. This yeast strain, which is a double mutant of the ERG6 (sterol transmethylase) and ERG2 (C-8 sterol isomerase) genes, accumulates zymosterol as its major sterol component.
Heiderpriem
R W
RW
Department of Chemistry, Auburn University, AL 36849.
Livant
P D
PD
Parish
E J
EJ
Barbuch
R J
RJ
Broaddus
M G
MG
Bard
M
M
D002784:Cholesterol; D012441:Saccharomyces cerevisiae / Q000737:chemistry*; D013261:Sterols / Q000302:isolation & purification*
D013261:Sterols; D002784:Cholesterol; C015582:zymosterol