Thiobacillus ferrooxidans detection using immunoelectron microscopy.
A specific, fast and very sensitive immunoelectron microscopy method was developed to morphologically and serologically distinguish different cultures of iron oxidizers. Bacteria isolated from the acidic waters of "Matahambre" and "Mina Delita" mines (Cuba) were characterized. An antiserum specific to Thiobacillus ferrooxidans did not react with other bacteria also present in the acidic waters of mine drainage. Our results suggest the occurrence of some strains of Thiobacillus ferrooxidans, Thiobacillus thiooxidans and Leptospirillum ferrooxidans in these waters.
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Departamento de Microbiología, Facultad de Biología, Universidad de La Habana, Ciudad de La Habana, Cuba.
Fernández
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León
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Rodríguez
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D000907:Antibodies, Bacterial / Q000276:immunology; D005296:Ferrous Compounds / Q000378:metabolism; D006046:Gold; D016253:Microscopy, Immunoelectron; D008906:Mining; D010084:Oxidation-Reduction; D012988:Soil Microbiology; D013855:Thiobacillus / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism / Q000648:ultrastructure; D014871:Water Microbiology
D000907:Antibodies, Bacterial; D005296:Ferrous Compounds; C020748:ferrous sulfate; D006046:Gold