Red blood cell protein map: a comparison between carrier-ampholyte pH gradient and immobilized pH gradient, and identification of four red blood cell enzymes.
The aim of this study was (a) to establish a red blood cell (RBC) protein map with immobilized pH gradient for the first dimension (b) to compare the pattern with previously published RBC protein map obtained with carrier-ampholyte pH gradients and (c) to localize four new enzymes on the map (i.e. 6-phosphogluconic dehydrogenase, glyceraldehyde-3-phosphate dehydrogenase, glutathione peroxidase and superoxide dismutase). This publication provides the most updated RBC polypeptide pattern with twelve proteins or enzymes localized on the map.
Golaz
O G
OG
Clinical Research Unit, Psychiatric University Institutions of Geneva, Switzerland.
Walzer
C
C
Hochstrasser
D
D
Bjellqvist
B
B
Turler
H
H
Balant
L
L
D001797:Blood Protein Electrophoresis / Q000379:methods*; D001798:Blood Proteins / Q000032:analysis*; D002021:Buffers; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D004912:Erythrocytes / Q000737:chemistry* / Q000201:enzymology; D005954:Glucosephosphate Dehydrogenase / Q000097:blood; D005979:Glutathione Peroxidase / Q000097:blood; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing / Q000379:methods*; D010734:Phosphogluconate Dehydrogenase / Q000097:blood; D012015:Reference Standards; D016622:Silver Staining; D013482:Superoxide Dismutase / Q000097:blood
D001798:Blood Proteins; D002021:Buffers; D010734:Phosphogluconate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase