title: Sterility of refrigerated injectable collagen syringes after injection of patient.
abstract: BACKGROUND
  Injectable collagen has been used for more than 15 years to correct soft tissue cosmetic defects. After injection, the collagen remaining in the syringe is often refrigerated for later use in the same patient, despite manufacturer and Food and Drug Administration recommendations to discard the unused collagen.
  
  
  OBJECTIVE
  This study examined the incidence of bacterial contamination of stored collagen.
  
  
  METHODS
  Syringe needle tips and collagen from 50 previously used syringes containing either Zyderm I, Zyderm II, or Zyplast were cultured separately under aerobic and anaerobic conditions. Bacterial isolates were identified.
  
  
  RESULTS
  Propionibacterium acnes was cultured from 7 of 50 needle tips. One positive needle tip culture grew both P. acnes and Staphylococcus aureus. Bacteria were isolated from only one collagen sample that grew a nonhemolytic streptococcus that may have represented a laboratory contaminant.
  
  
  CONCLUSIONS
  Syringes of collagen stored for repeated use rarely become contaminated with bacteria despite frequent contamination of their needle tips. Skin abscesses after collagen injection should be cultured under anaerobic, as well as aerobic, conditions so that infections caused by P. acnes will not be missed.
authors: Department of Dermatology, Mount Sinai Medical Center, New York, NY.
authors: K
authors: K
authors: Davis
authors: E J
authors: EJ
authors: Bottone
authors: D
authors: D
authors: Lucas
authors: M
authors: M
authors: Lebwohl
mesh_terms: D003094:Collagen / Q000008:administration & dosage*; D004340:Drug Contamination; D004356:Drug Storage; D006801:Humans; D011425:Propionibacterium acnes / Q000302:isolation & purification; D012034:Refrigeration; D013211:Staphylococcus aureus / Q000302:isolation & purification; D013594:Syringes
chemical_list: D003094:Collagen