title: Simultaneous determination of catecholamines and dobutamine in human plasma and urine by high-performance liquid chromatography with fluorimetric detection.
abstract: We report a reliable fluorimetric assay for the simultaneous determination of norepinephrine, epinephrine, dopamine and dobutamine in human plasma and urine, based on liquid-liquid extraction and derivatization with the fluorogenic agent 1,2-diphenylethylenediamine prior to chromatography. The method is sensitive (detection limit 0.3-0.8 pg injected) and reproducible (coefficients of variation 1-10%), and shows good accuracy (93-98%). The method should also be used when one only wants to measure the concentrations of the natural catecholamines, in order to avoid interference by metabolites of dobutamine and by the late-eluting dobutamine itself.
authors: Department of Internal Medicine I, University Hospital Dijkzigt, Erasmus University, Rotterdam, Netherlands.
authors: G
authors: G
authors: Alberts
authors: F
authors: F
authors: Boomsma
authors: A J
authors: AJ
authors: Man in 't Veld
authors: M A
authors: MA
authors: Schalekamp
mesh_terms: D002395:Catecholamines / Q000032:analysis* / Q000097:blood / Q000652:urine; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D004280:Dobutamine / Q000032:analysis* / Q000097:blood / Q000652:urine; D006801:Humans; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence
chemical_list: D002395:Catecholamines; D004280:Dobutamine