Dopa-decarboxylation in the striata of rats with unilateral substantia nigra lesions.
The source and site of the DOPA decarboxylation to dopamine in Parkinson's disease (PD) and animal models of PD are controversial. Since most of aromatic L-amino acid decarboxylase (AADC) are lost along with the degenerating dopaminergic neurons, we addressed the possibility that other decarboxylases or a novel protein that is structurally different from AADC decarboxylate L-DOPA in the denervated striatum. Immunotitration of the extracts from the denervated striatum with AADC antibody showed that all activity can be attributed to AADC-immunoreactive protein. We then investigated if there are non-dopaminergic intrinsic striatal neurons that express AADC. No evidence of such neurons was noted by immunocytochemistry and in situ hybridization.
Kang
U J
UJ
Burke Medical Research Institute, Cornell University Medical College, White Plains, NY 10605.
Park
D H
DH
Wessel
T
T
Baker
H
H
Joh
T H
TH
D000818:Animals; D001142:Aromatic-L-Amino-Acid Decarboxylases / Q000378:metabolism; D003342:Corpus Striatum / Q000201:enzymology / Q000378:metabolism*; D003653:Decarboxylation; D003714:Denervation; D003956:Dialysis; D004295:Dihydroxyphenylalanine / Q000378:metabolism*; D004296:Dopa Decarboxylase / Q000378:metabolism; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008297:Male; D051381:Rats; D013378:Substantia Nigra / Q000201:enzymology / Q000378:metabolism*
D004295:Dihydroxyphenylalanine; D004296:Dopa Decarboxylase; D001142:Aromatic-L-Amino-Acid Decarboxylases