Insecticide resistance gene frequencies in Anopheles sacharovi populations of the Cukurova plain, Adana Province, Turkey.
In Turkey, the mosquito Anopheles sacharovi has been under field selection pressure sequentially with DDT, dieldrin, malathion and pirimiphosmethyl over a period of 30 years for the purpose of malaria control. In 1984, the field population of An.sacharovi in the malarious Cukurova plain of Adana Province contained an altered acetylcholinesterase-based resistance gene giving broad spectrum resistance against organophosphorus and carbamate insecticides. The cross-resistance spectrum from this mechanism conferred resistance to malathion but not to the organophosphorus insecticide pirimiphos-methyl. Over the 6 years that pirimiphos-methyl has been applied for malaria vector control in this area, the frequency of the altered acetylcholinesterase resistance gene has declined, although in 1989 and 1990 it was still present at measurable frequencies in An.sacharovi from Cukurova. In addition to the acetylcholinesterase resistance mechanism there is evidence of an increased level of glutathione S-transferase in some of the An.sacharovi populations tested. This is known to be correlated with DDT resistance in other anophelines. In Turkish An.sacharovi, DDT resistance and elevated glutathione S-transferase occur in the same populations at similar frequencies. The continued prevalence of resistance to DDT and dieldrin, long after the 1971 cessation of DDT spraying for malaria control in Turkey, suggests that the DDT resistance gene has insufficient reduced fitness associated with it to have been lost from the field population during the past two decades. The implications of the slow decline in resistance gene frequencies in this field population are discussed in relation to mathematical models for managing resistance.
Hemingway
J
J
Department of Medical Parasitology, London School of Hygiene and Tropical Medicine, U.K.
Small
G J
GJ
Monro
A
A
Sawyer
B V
BV
Kasap
H
H
D000110:Acetylcholinesterase / Q000032:analysis; D000818:Animals; D000852:Anopheles / Q000201:enzymology / Q000235:genetics*; D001681:Biological Assay; D003577:Cytochrome P-450 Enzyme System / Q000032:analysis; D004950:Esterases / Q000032:analysis; D005260:Female; D005787:Gene Frequency / Q000235:genetics*; D005982:Glutathione Transferase / Q000032:analysis; D007305:Insecticide Resistance / Q000235:genetics*; D014421:Turkey
D003577:Cytochrome P-450 Enzyme System; D005982:Glutathione Transferase; D004950:Esterases; D000110:Acetylcholinesterase