In vitro effect of hCG on steroidogenesis in the testicular tissue from a patient with complete androgen resistance.
The present study was performed to determine the in vitro steroidogenic capacity of a gonadal sample from a patient suffering from a complete androgen resistance syndrome. Testosterone and estradiol production by the testicular tissue from this patient as well as gonadotropin binding to a membrane fraction prepared from this tissue were measured. hCG bound with high affinity but with a very low capacity and the gonadotropin induced a clear dose response for both testosterone and estradiol production. The ED50 of hCG on testosterone and estradiol production were 2.5 and 5.0 nM, respectively. We conclude that estradiol originates from Leydig cell activity, since estradiol synthesis does not depend on testosterone availability and it shows a clear hCG dose response.
Ronco
A M
AM
Instituto de Nutricion y Tecnologia de los Alimentos, Universidad de Chile, Casilla, Santiago.
Valladares
L E
LE
Devoto
L
L
Ahumada
A
A
Pino
A M
AM
D000293:Adolescent; D006063:Chorionic Gonadotropin / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004958:Estradiol / Q000096:biosynthesis*; D005260:Female; D006061:Gonadal Dysgenesis, 46,XY; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D013737:Testis / Q000002:abnormalities / Q000187:drug effects / Q000378:metabolism*; D013739:Testosterone / Q000096:biosynthesis*
D006063:Chorionic Gonadotropin; D013739:Testosterone; D004958:Estradiol