Simultaneous determination of retinol and tocopherols by high-performance liquid chromatography.
A high-performance liquid chromatographic (HPLC) method to determine retinol and all four tocopherols (alpha-, beta-, gamma- and delta-) simultaneously was established using a reversed-phase column (YMC-PACK A-302 S-5 120A ODS). The HPLC conditions were mobile phase 65% isopropanol, sample solvent 99.5% methanol and temperature 30 degrees C. Retinol and tocopherols were measured in rat liver.
Satomura
Y
Y
Department of Hygiene, Faculty of Medicine, Kyoto University, Japan.
Kimura
M
M
Itokawa
Y
Y
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D008099:Liver / Q000737:chemistry; D051381:Rats; D012680:Sensitivity and Specificity; D014801:Vitamin A / Q000032:analysis*; D014810:Vitamin E / Q000032:analysis*
D014801:Vitamin A; D014810:Vitamin E