[The effects of potassium concentration in reperfusion solution upon myocardial protection].
The effects of potassium in reperfusion solution (RS) and the influence of sodium on this effect were studied. Experimental time course was as followed: 20 min working perfusion, 3 min cardioplegic infusion with St. Thomas Cardioplegic Solution followed by global ischemia for 33 or 35 min at 37.5 degrees C, 15 min early Langendorff reperfusion with several different potassium concentration modified with Krebs Henseleit Bicarbonate Buffer (KHBB) containing 145 mM and 110 mM sodium and 5 min late reperfusion with KHBB, followed by 20 min working perfusion. Potassium in RS possessed bell shaped dose response nature with optimal concentration of 10 mM in the condition of 145 mM sodium but 6 m in the condition of 110 mM in terms of percent recovery of aortic flow. Although higher potassium reperfusion produced less Creatine Kinase leakage.
Ohashi
T
T
Department of Cardiovascular Surgery, National Cardiovascular Center, Osaka, Japan.
Yamamoto
F
F
Yamamoto
H
H
Ichikawa
H
H
Shibata
T
T
Shimada
Y
Y
Ishikawa
T
T
Kagisaki
K
K
Kumada
Y
Y
Kawashima
Y
Y
D000818:Animals; D001639:Bicarbonates / Q000737:chemistry; D002122:Calcium Chloride / Q000737:chemistry; D002314:Cardioplegic Solutions; D003402:Creatine Kinase / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D066298:In Vitro Techniques; D008274:Magnesium / Q000737:chemistry; D008297:Male; D015425:Myocardial Reperfusion; D015428:Myocardial Reperfusion Injury / Q000517:prevention & control; D009206:Myocardium / Q000378:metabolism; D011188:Potassium / Q000032:analysis / Q000494:pharmacology*; D011189:Potassium Chloride / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D012965:Sodium Chloride / Q000737:chemistry; D014022:Tissue Survival
D001639:Bicarbonates; D002314:Cardioplegic Solutions; C041711:St. Thomas' Hospital cardioplegic solution; D012965:Sodium Chloride; D011189:Potassium Chloride; D003402:Creatine Kinase; D008274:Magnesium; D002122:Calcium Chloride; D011188:Potassium