title: Low temperature induced non-culturability and killing of Vibrio vulnificus.
abstract: Vibrio vulnificus cells progressively lose culturability during incubation at 5 degrees C. This process is accelerated by the addition of supernatants from non-culturable cells obtained by incubation at 5 degrees C for 17 days. Thus the organism apparently produces a factor upon cold incubation which is triggering or causing the decline in culturability. Reversing the temperature shift can restore a culturable population comparable in numbers to the original population, but this process is largely due to regrowth. A few cells retaining the ability to grow apparently utilize the substrates released by the moribund cells, thus mimicking resuscitation of the whole population.
authors: Department of General and Marine Microbiology, University of Göteborg, Sweden.
authors: D
authors: D
authors: Weichart
authors: J D
authors: JD
authors: Oliver
authors: S
authors: S
authors: Kjelleberg
mesh_terms: D003080:Cold Temperature; D015169:Colony Count, Microbial; D003470:Culture Media; D014733:Vibrio / Q000166:cytology / Q000254:growth & development*
chemical_list: D003470:Culture Media