title: In vitro model of essential fatty acid deficiency.
abstract: The polyunsaturated fatty acids linoleic acid (18:2, n-6) and arachidonic acid (20:4, n-6) are essential for normal skin function and structure, both as eicosanoid precursors and as components of lipids forming cell membranes. Adult human keratinocytes grow optimally in serum-free medium (MCDB 153) that contains no fatty acids. These keratinocytes expand rapidly and produce normal epidermis upon in vivo grafting. Analysis of lipid extracts of epidermis and of cultured keratinocytes was done to determine the fatty acid composition of cells grown in essential fatty acid (EFA)-deficient medium. Gas chromatography and high-performance liquid chromatography analyses were done of the fatty acids in the entire cell and in a thin-layer chromatography separated fraction containing those lipids that form cellular membranes. Comparison of snap-frozen epidermis and epidermal basal cell suspensions to passage 1 to 4 cultures shows that the cells are in an extreme essential fatty acid-deficient state by the first passage. The amount of the saturated fatty acids 16:0, 18:0, and 14:0 is unchanged by culture. The polyunsaturated fatty acids are found to be significantly decreased, the cells balancing their lack with a significant increase in the relative abundance of the monounsaturated fatty acids, 18:1 and 16:1. Greater than 85-90% of the fatty acids was found in lipids associated with membranes and no unusual fatty acids were detected. Because the serum-free medium is fatty acid free and the cells cannot synthesize essential fatty acids, the rapid division of the cells results in the predominance of an extreme EFA-deficient cell type. The essential fatty acid-deficient keratinocyte is an excellent adult, normal epidermal cell model that can be used to study EFA deficiency and the effect of the eicosanoid and fatty acids on cell function and structure.
authors: University of Michigan Medical School, Department of Dermatology, Ann Arbor 48109-0528.
authors: C L
authors: CL
authors: Marcelo
authors: E A
authors: EA
authors: Duell
authors: L M
authors: LM
authors: Rhodes
authors: W R
authors: WR
authors: Dunham
mesh_terms: D000818:Animals; D001706:Biopsy; D002417:Cattle; D002460:Cell Line; D002849:Chromatography, Gas; D016895:Culture Media, Serum-Free; D005227:Fatty Acids / Q000032:analysis; D005228:Fatty Acids, Essential / Q000172:deficiency*; D006801:Humans; D015603:Keratinocytes / Q000737:chemistry / Q000166:cytology; D010743:Phospholipids / Q000737:chemistry; D010902:Pituitary Gland / Q000737:chemistry; D012867:Skin / Q000473:pathology; D014020:Tissue Extracts / Q000737:chemistry
chemical_list: D016895:Culture Media, Serum-Free; D005227:Fatty Acids; D005228:Fatty Acids, Essential; D010743:Phospholipids; D014020:Tissue Extracts