title: Site-dependent effects of experimental hypo- and hyperthyroidism on resident macrophages in extraocular muscles of rats: a quantitative immunohistochemical study.
abstract: It has been suggested that the effects of dysthyroidism on resident immunocompetent cells of the extraocular muscles may play a role in the pathogenesis of Graves' ophthalmopathy. The distribution of such cells was therefore studied in extraocular muscles of rats that were made hyper- or hypothyroid by the oral administration of thyroxine or propylthiouracil respectively. Skeletal muscles were studied for comparison. The cell distributions were analysed in cryostat cross-sections subjected to a two-step immunoperoxidase method using well-characterized monoclonal antibodies against T cells, B cells, macrophages and MHC class II antigens. The extraocular muscles of control (euthyroid) rats contained numerous macrophages, fewer MHC-II positive cells and T cells and no B cells. Differences in the distribution of immunocompetent cells were found in control rats, between skeletal and extraocular muscles as well as within the various recti eye muscles. This particular tissue distribution resembles that previously reported for human extraocular and skeletal muscles. Quantitative analysis showed that experimental dysthyroidism only affected cell populations in the extraocular muscles. Significant effects on the number of macrophages were observed in the inferior rectus muscle of both hypo- and hyperthyroid rats, this was most pronounced in the orbital layer of the muscles. Both hyper- and hypothyroidism appear to affect local cell distributions in a tissue-specific manner. The presently observed site-dependent effects of dysthyroidism on local immunocompetent cell populations may have relevance for the differential involvement of muscular tissues in Graves' ophthalmopathy.
authors: Department of Ophthalmology, Academic Medical Centre, University of Amsterdam, The Netherlands.
authors: E D
authors: ED
authors: Schmidt
authors: G
authors: G
authors: van Hogerwou
authors: R
authors: R
authors: van der Gaag
authors: W M
authors: WM
authors: Wiersinga
authors: G
authors: G
authors: Asmussen
authors: L
authors: L
authors: Koornneef
mesh_terms: D000818:Animals; D002452:Cell Count; D005123:Eye / Q000473:pathology*; D006111:Graves Disease / Q000473:pathology*; D006980:Hyperthyroidism / Q000473:pathology; D007037:Hypothyroidism / Q000473:pathology; D007150:Immunohistochemistry; D008264:Macrophages / Q000473:pathology*; D008297:Male; D009132:Muscles / Q000473:pathology*; D051381:Rats; D017208:Rats, Wistar; D013959:Thyroid Diseases / Q000473:pathology*