Structure of a Plasmodium chabaudi acidic phosphoprotein that is associated with the host erythrocyte membrane.
We have characterized by molecular cloning and sequencing a Plasmodium chabaudi antigen that is associated with the membrane of the infected erythrocyte throughout the entire intraerythrocytic cycle. The protein (PcEMA1) has a predicted size of 50 kDa and contains a major tandem repeat array of 16 octapeptides that constitutes almost 30% of the protein. At its amino-terminus, PcEMA1 has a string of hydrophobic residues characteristic of a secreted protein, but does not contain a hydrophobic membrane-spanning segment. The antigen appears to reside on the cytoplasmic face of the erythrocytic membrane. PcEMA1 has a predicted pI of 4.4 and is a potential phosphoprotein.
Deleersnijder
W
W
Laboratorium Algemene Biologie, Instituut voor Moleculaire Biologie, Vrije Universiteit Brussel, Belgium.
Prasomsitti
P
P
Tungpradubkul
S
S
Hendrix
D
D
Hamers-Casterman
C
C
Hamers
R
R
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D016054:DNA, Protozoan / Q000235:genetics; D004910:Erythrocyte Membrane / Q000378:metabolism; D017125:Genes, Protozoan; D006790:Host-Parasite Interactions; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010750:Phosphoproteins / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D016788:Plasmodium chabaudi / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D015800:Protozoan Proteins / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D012091:Repetitive Sequences, Nucleic Acid
D016054:DNA, Protozoan; D010750:Phosphoproteins; D015800:Protozoan Proteins