A DNA fragment homologous to F1-ATPase beta subunit was amplified from genomic DNA of Methanosarcina barkeri. Indication of an archaebacterial F-type ATPase.
A 490 bp DNA fragment was amplified from Methanosarcina barkeri genomic DNA by the polymerase chain reaction (PCR) using oligonucleotide primers designed based on conserved amino acid sequences of the F1-ATPase beta subunits. The amino acid sequence deduced from the DNA sequence of this fragment was highly homologous to a portion of the F1-ATPase beta subunit. This indicates that this archaebacterium has a gene of F-type ATPase in addition to a gene of V-type ATPase.
Sumi
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Research Laboratory of Resources Utilization, Tokyo Institute of Technology, Yokohama.
Sato
M H
MH
Denda
K
K
Date
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Yoshida
M
M
D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D004277:DNA, Single-Stranded; D017021:Methanosarcina barkeri / Q000201:enzymology / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D006180:Proton-Translocating ATPases / Q000235:genetics* / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid
D004269:DNA, Bacterial; D004277:DNA, Single-Stranded; D006180:Proton-Translocating ATPases