Determination of carcinogenic potency of alkytoxynol-741 (AP-741) by rat peritoneal cell cultures.
The carcinogenic potency of AP-741 was tested in rats using the Nashed method of rat peritoneal cell short-term carcinogenic test. N-methyl-N-nitro-N'-nitrosoguanidian (MNNG) was used as positive control; saline and nonoxynol-9 (NP-9) were used as negative control. Two doses of AP-741 (4 mg/kg and 40 mg/kg equivalent to 4 and 40 times human doses) were tested. The result showed that no colonies of more than 9 cells were seen in the saline and NP-9 groups, and the two dose groups of AP-741. However, 12 +/- 8.3 colonies (9-29 cell/colony) and 4.5 +/- 4.2 colonies (30-300 cell/colony) were seen in the MNNG group. According to the Nashed criterion, we can say MNNG has potential carcinogenesis, while AP-741 does not have any potential carcinogenesis and its use as a vaginal contraceptive drug is safe.
Qin
J
J
Tianjin Municipal Research Institute for Family Planning, China.
Wang
C
C
He
Q
Q
Diao
X
X
D000818:Animals; D002273:Carcinogens / Q000633:toxicity*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005260:Female; D011092:Polyethylene Glycols / Q000009:adverse effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013089:Spermatocidal Agents / Q000633:toxicity*
D002273:Carcinogens; D013089:Spermatocidal Agents; C068240:agent 741; D011092:Polyethylene Glycols