title: Molecular cloning and nucleotide sequence of cDNA encoding a functional murine low-density-lipoprotein receptor.
abstract: The low-density-lipoprotein (LDL) receptor is an important mediator of mammalian cholesterol metabolism; its congenital absence in humans is characterized by hypercholesterolemia, atherosclerosis, and coronary artery disease. We report here the identification and cloning of a cDNA encoding the murine LDL receptor. The cDNA insert is 4467 base pairs in length and the deduced amino acid sequence bears 78.2% homology with the reported human sequence. This murine cDNA was subcloned into a retroviral-based expression vector, LmLSN1, and transfected into 3T3 cells. The production of functional LDL receptor was confirmed by ligand binding of DiI-LDL cholesterol.
authors: Molecular Hematology Branch, National Heart Lung and Blood Institute, Bethesda, Maryland 20892.
authors: W J
authors: WJ
authors: Polvino
authors: D A
authors: DA
authors: Dichek
authors: J
authors: J
authors: Mason
authors: W F
authors: WF
authors: Anderson
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D005796:Genes; D005822:Genetic Vectors; D007438:Introns; D008077:Lipoproteins, LDL / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D011973:Receptors, LDL / Q000235:genetics* / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D012190:Retroviridae; D017386:Sequence Homology, Amino Acid
chemical_list: D008077:Lipoproteins, LDL; D011973:Receptors, LDL; D011994:Recombinant Proteins; D004247:DNA