Mesencephalic cuneiform nucleus and its ascending and descending projections serve stress-related cardiovascular responses in the rat.
The aim of the present study was to explore the neuroanatomic network that underlies the cardiovascular responses of reticular formation origin in the region of the cuneiform nucleus (CNF). The study was performed in urethane anesthetized male Wistar rats. The left iliac artery was supplied with a catheter for the measurement of systemic blood pressure. Low intensity electrical stimulation of the mesencephalic reticular formation (MRF) in the vicinity of the CNF always resulted in pressor and bradycardiac responses, whereas stimulation in the parabrachial nucleus (PB) and Kölliker-Fuse nucleus (KF) led to a pressor response and a small tachycardiac response. The cuneiform area may be placed in the center of a circuit that serves a specific autonomic response pattern to stress: parallel activation of the sympathetic (pressor response) and parasympathetic limb (bradycardia). The efferent connections of the effective stimulation sites in the MRF and the CNF area, were investigated by anterograde tracing with the lectin Phaseolus vulgaris leucoagglutine (PHA-L). The CNF sends descending fibers to the gigantocellular reticular nuclei (GI), the motor nucleus of the vagus (DMNV) and nucleus tractus solitarius (NTS). These projections are probably involved in the bradycardiac response to stimulation. The descending pathway to the NTS/DMNV and GI may therefore be the parasympathetic limb of the circuit. Furthermore, the CNF sends ascending fibers to limbic forebrain areas and descending fibers to the PB-KF complex. The KF in its turn projects to the rostroventrolateral medullary nucleus (RVLM) and the intermediolateral cell column (IML). These latter projections are partly involved in producing the pressor response and thereby represent the sympathetic limb of the circuit. Accordingly, the transection of the descending fibers from the CNF to the PB-KF complex resulted in a decreased pressor and an increased bradycardiac response. This suggests that a baroreceptor reflex-induced bradycardia which results from blood pressure increase can be excluded as the origin of the stimulation-induced bradycardia, and that the pressor and bradycardiac responses are two independent moieties. It cannot be excluded that ascending fibers from the CNF are also involved in producing the pressor response. On the basis of the present physiological and neuroanatomical study, a brain circuit has been proposed in which the cuneiform nucleus has a central position. The described brain circuit may serve a passive coping strategy to novel, painful or threatening stimuli during which the animals show orientation/attention or freezing behavior accompanied by a bradycardiac and pressor response.
Korte
S M
SM
Department of Animal Physiology, University of Groningen, Haren, Netherlands.
Jaarsma
D
D
Luiten
P G
PG
Bohus
B
B
D000223:Adaptation, Psychological / Q000502:physiology; D000344:Afferent Pathways / Q000502:physiology; D000818:Animals; D001369:Axons / Q000502:physiology; D001794:Blood Pressure / Q000502:physiology; D004525:Efferent Pathways / Q000502:physiology; D004558:Electric Stimulation; D004566:Electrodes; D006339:Heart Rate / Q000502:physiology; D006439:Hemodynamics / Q000502:physiology*; D007150:Immunohistochemistry; D008297:Male; D008636:Mesencephalon / Q000473:pathology / Q000503:physiopathology*; D010835:Phytohemagglutinins; D051381:Rats; D017208:Rats, Wistar; D012154:Reticular Formation / Q000033:anatomy & histology / Q000502:physiology; D013315:Stress, Psychological / Q000503:physiopathology*
D010835:Phytohemagglutinins; C100156:leukoagglutinins, plants