Can the mouse embryo provide a good model for the study of abnormal cellular development seen in human embryos?
Mouse 2-cell embryos arrested in development, either due to the effect of in vitro culture conditions ('2-cell block') or after exposure to the protein synthesis inhibitor anisomycin, were examined to determine the effect of the level of protein synthesis on development. The rate of protein synthesis was found directly to reflect the developmental potential of the embryos. Embryos cultured in the highest dose of the drug failed to divide and had the lowest rate of protein synthesis over the period of investigation, whereas untreated viable 2-cell embryos in the control group had the highest rate of protein synthesis and developed normally. Measurement of the nuclear DNA showed that both arrested and non-arrested embryonic cells completed DNA replication. Furthermore, drug-arrested embryos, like embryos which 'block' in culture, remained morphologically intact when left in culture. Disruption of the nuclear integrity and formation of micronuclei, as is frequently observed in arrested human embryos, was not seen in mouse embryos. These results indicate that developmentally arrested mouse embryos may not be a good model for studying cellular dysfunction in early human development. Experimentation using human material is required to address directly the problem of abnormal human development.
Winston
N J
NJ
Department of Anatomy, University of Cambridge, UK.
Johnson
M H
MH
D000818:Animals; D000841:Anisomycin; D002455:Cell Division / Q000187:drug effects; D002970:Cleavage Stage, Ovum / Q000187:drug effects / Q000378:metabolism*; D004247:DNA / Q000096:biosynthesis; D004305:Dose-Response Relationship, Drug; D051379:Mice / Q000196:embryology*; D008954:Models, Biological; D014176:Protein Biosynthesis; D012323:RNA Processing, Post-Transcriptional / Q000187:drug effects
D000841:Anisomycin; D004247:DNA