Selective purification of two distinct protein kinases (C-kinase and casein kinase II) from the membrane fraction of mouse brain by NED-affinity column chromatography.
By means of NED-affinity column chromatography, two distinct protein kinases have been selectively purified from the crude membrane extract of mouse brain. One (designated P-I kinase) was eluted from the column by the buffer containing 5 mM EGTA and the other (designated P-II kinase) was eluted by the buffer containing 0.6 M KCl. The activity of A-kinase was detected in the column passed through fraction. Biochemical characteristics of P-I and P-II kinases corresponded exactly to those of C-kinase and casein kinase II (CK-II), respectively. In addition, immunoprecipitate experiment using anti-CK-II antiserum against the beta-subunit of Drosophila CK-II showed that P-II kinase is identical to CK-II and the 62 kDa cellular polypeptide is associated with the kinase.
Kanno
S
S
Department of Pharmacological Sciences, Tohoku University Hospital, Sendai, Japan.
Mizugaki
M
M
Maruyama
T
T
Ohtsuki
K
K
D000818:Animals; D001921:Brain / Q000201:enzymology*; D047390:Casein Kinase II; D002462:Cell Membrane / Q000201:enzymology; D002846:Chromatography, Affinity; D005029:Ethylenediamines; D008565:Membrane Proteins / Q000302:isolation & purification*; D051379:Mice; D009419:Nerve Tissue Proteins / Q000302:isolation & purification*; D011493:Protein Kinase C / Q000302:isolation & purification*; D017346:Protein Serine-Threonine Kinases / Q000302:isolation & purification*
D005029:Ethylenediamines; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; C008588:N-(1-naphthyl)ethylenediamine; D047390:Casein Kinase II; D017346:Protein Serine-Threonine Kinases; D011493:Protein Kinase C