Microtubular tau protein after cocaine in cultured SH-SY5Y human neuroblastoma.
1. SH-SY5Y, a human neuroblastoma cell line, was used as a tissue culture model to examine the hypothesis that cocaine may affect the metabolism of tau protein which stabilizes microtubules and promotes microtubule assembly. 2. Cocaine hydrochloride (10(-9)-10(-3) M) caused dose-dependent reductions in cell number, with 10(-3) M causing 28% reduction after 48 hr. 3. This drug also decreased tau protein (50 Kd) in the cytoplasmic (supernatant) as well as the membrane (pellet) fraction after 48-hr treatment.
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Department of Anatomy, College of Human Medicine, Michigan State University, E. Lansing 48824.
D015153:Blotting, Western; D001932:Brain Neoplasms / Q000378:metabolism*; D003042:Cocaine / Q000494:pharmacology*; D003593:Cytoplasm / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008870:Microtubules / Q000187:drug effects / Q000378:metabolism*; D009447:Neuroblastoma / Q000378:metabolism*; D014407:Tumor Cells, Cultured; D016875:tau Proteins / Q000096:biosynthesis*
D016875:tau Proteins; D003042:Cocaine