Legionella pneumophila species identification using a commercial latex agglutination kit: a potential cross-reaction problem with serogroup 12.
Seventy-nine strains of legionella spp. and 13 non-legionella isolates were tested using a commercial latex agglutination kit. Cross-reactions were observed for all strains of Legionella pneumophila serogroup 12 examined between latex particles coated with rabbit antibodies raised against L. pneumophila serogroup 1 and those coated with rabbit antibodies to serogroups 2-14. Strains of L. pneumophila serogroup 1 did not cross-react with latex particles directed against L. pneumophila serogroups 2-14. This suggests that L. pneumophila serogroup 12 shares common surface antigens with L. pneumophila serogroup 1, and that conversely serogroup 1 isolates do not share major surface antigen determinants with serogroups 2-14. All other isolates tested gave expected results.
Richardson
I R
IR
Newcastle Public Health Laboratory, Institute of Pathology, General Hospital, Newcastle upon Tyne, England, UK.
Lightfoot
N F
NF
D000942:Antigens, Bacterial / Q000276:immunology; D000954:Antigens, Surface / Q000276:immunology; D003429:Cross Reactions; D007841:Latex Fixation Tests / Q000379:methods; D016952:Legionella pneumophila / Q000145:classification / Q000276:immunology / Q000302:isolation & purification*; D012703:Serotyping
D000942:Antigens, Bacterial; D000954:Antigens, Surface