Enhancing effects of fluorescein on beta-lactam rash. II: Enhancing effects of fluorescein on generalized rash induced by beta-lactam antibiotics in guinea pigs.
Healthy volunteers, who were receiving intravenous injections of cefclidin (CFCL) with frequent concomitant use of fluorescein (F) and oxybuprocain (O) in the eyes for measurement of ocular tension, developed drug eruptions at the high frequency of 66.7%. The injection of CFCL alone induced the eruptions at an incidence of 2.8%. The cause of this high eruption rate was thought to be the simultaneous treatment with F and/or O. Therefore, we conducted experiments with CFCL-induced generalized rash (GR) in guinea pigs. Guinea pigs treated with F and O during both the phases of immunization and intraperitoneal elicitation developed CFCL rashes at a high frequency. This CFCL-rash was augmented by the treatment with F during either phase, but not by the treatment with O. Skin testing induced delayed type hypersensitivity (DTH) reaction to O in some animals, but the DTH to F was not induced in animals immunized with F in complete Freund's adjuvant. Furthermore, F augmented rashes induced not only by CFCL but also by other beta-lactam antibiotics such as cefsulodin and sulbenicillin. Accordingly, it is likely that F played a dominant role in the high incidence of drug eruptions during the volunteer trials with measurement of ocular tension.
Ikezawa
Z
Z
Yokohama City University School of Medicine, Urafune Hospital, Department of Dermatology, Japan.
Sugihara
Y
Y
Ueno
J
J
D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000009:adverse effects; D002511:Cephalosporins / Q000008:administration & dosage / Q000009:adverse effects*; D004195:Disease Models, Animal; D003875:Drug Eruptions / Q000175:diagnosis / Q000209:etiology*; D004347:Drug Interactions; D005452:Fluoresceins / Q000008:administration & dosage / Q000009:adverse effects*; D006168:Guinea Pigs; D008297:Male
D000900:Anti-Bacterial Agents; D002511:Cephalosporins; D005452:Fluoresceins; C056494:cefclidin