Superimposing two-dimensional gels to study genetic variation in malaria parasites.
Two-dimensional polyacrylamide gel electrophoresis is a valuable tool for studying genetic variation in the human malaria parasite, Plasmodium falciparum. It involves examining the position of protein spots in gel produced from different isolates. Some spots have been seen to vary, while others have had a constant position in all isolates so far examined. These invariant spots provide a reference frame to compare variations in other spots. This paper discusses the usefulness of digital image handling, warping and superimposition in a personal computer environment. Rather than produce a fully automatic interpretation system, we show how the computer may be used as a tool for manipulating gel images, although interpretation of the gels' features remains with the human expert. Autoradiographs are scanned on a desktop scanner, and the images in digital form can be displayed on a monitor attached to a personal computer. The coordinates of the invariant spots on each of several gels are identified by the user. Each of the gels is then warped so that the invariant spots of all the gels coincide as closely as possible. The variable spots are then examined. We have used both affine warping transformations, which match the invariant spots as closely as possible, and thin plate spline transformations, which match them exactly. Colour superimposition proved a useful way of examining the gels.
Horgan
G
G
Scottish Agricultural Statistics Service, Edinburgh.
Creasey
A
A
Fenton
B
B
D000818:Animals; D015180:Electrophoresis, Gel, Two-Dimensional; D014644:Genetic Variation / Q000235:genetics*; D007091:Image Processing, Computer-Assisted; D010963:Plasmodium falciparum / Q000235:genetics*; D015800:Protozoan Proteins / Q000032:analysis*; D012015:Reference Standards
D015800:Protozoan Proteins