title: Properties of bolesatine, a translational inhibitor from Boletus satanas Lenz. Amino-terminal sequence determination and inhibition of rat mitochondrial protein synthesis.
abstract: Bolesatine is a toxic monomeric glycoprotein of Mr 63,000 isolated from the mushroom Boletus satanas Lenz. Its N-terminal amino acid sequence was established: NH2-Thr-Trp-Arg-Ile-Tyr-Leu-Asn-Asn-Gln-Thr-Val-Lys-Leu-Ala-Leu-Leu-Leu- Pro- Asn-Gly.... It inhibits protein synthesis in isolated rat mitochondria. After 90 min of preincubation with bolesatine, the incorporation of [14C]-leucine into mitochondrial proteins was inhibited with an IC50 of 530 nM.
authors: Institut de Biologie Moléculaire et Cellulaire du Centre National de la Recherche Scientifique, Strasbourg, France.
authors: O
authors: O
authors: Kretz
authors: J
authors: J
authors: Reinbolt
authors: E E
authors: EE
authors: Creppy
authors: G
authors: G
authors: Dirheimer
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D005656:Fungal Proteins / Q000737:chemistry / Q000494:pharmacology*; D066298:In Vitro Techniques; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism; D008969:Molecular Sequence Data; D009183:Mycotoxins / Q000737:chemistry / Q000494:pharmacology*; D014176:Protein Biosynthesis / Q000187:drug effects*; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology*; D051381:Rats
chemical_list: D005656:Fungal Proteins; D009183:Mycotoxins; D011500:Protein Synthesis Inhibitors; C060924:Bolesatine protein, Boletus satanas