DNA probes for the identification of members of the Simulium damnosum complex (Diptera: Simuliidae).
Genomic libraries in plasmid have been constructed from various sibling species of blackflies of the Simulium damnosum complex from West Africa. Three cloned repetitive sequences, which show variation in copy number between sibling species, have been isolated. These clones can be used as probes for the dot-blot identification of larvae, pupae or adults into the three main West African subcomplexes, i.e. damnosum, squamosum and sanctipauli subcomplexes. The sequences also show some intraspecific variation in copy number.
Post
R J
RJ
Department of Medical Entomology, Liverpool School of Tropical Medicine.
Flook
P
P
D000818:Animals; D004247:DNA / Q000737:chemistry; D015342:DNA Probes; D015698:Genomic Library; D015151:Immunoblotting; D009693:Nucleic Acid Hybridization; D012680:Sensitivity and Specificity; D012843:Simuliidae / Q000145:classification* / Q000235:genetics; D013816:Thermodynamics
D015342:DNA Probes; D004247:DNA