Identification and localization of microsatellite markers covering human chromosome 18.
To generate microsatellite markers from chromosome 18, we have cytogenetically localized a large number of lambda phage using a deletion mapping panel of somatic cell hybrids. Here we describe the identification of 65 new CA-repeat-containing phage and the localization of five markers developed in other laboratories. This approach allows the selection of a subset of markers that are well spaced across the chromosome and can be developed as genetic markers. The use of PCR-based markers should allow for the rapid genomic screening of disease genes on chromosome 18.
Rojas
K
K
Department of Biochemistry and Molecular Biology, Thomas Jefferson University, Philadelphia, Pennsylvania 19107.
Straub
R E
RE
Kurtz
A
A
Feder
M
M
Mewar
R
R
Gilliam
T C
TC
Overhauser
J
J
D001483:Base Sequence; D002874:Chromosome Mapping; D002887:Chromosomes, Human, Pair 18; D004276:DNA, Satellite / Q000235:genetics*; D005819:Genetic Markers; D006801:Humans; D006822:Hybrid Cells; D008969:Molecular Sequence Data; D009841:Oligonucleotides
D004276:DNA, Satellite; D005819:Genetic Markers; D009841:Oligonucleotides