Evaluation of enzyme immunoassay using a recombinant envelope protein expressed in insect cells for serological confirmation of HTLV-I infection.
A recombinant human T-lymphotropic virus type I (HTLV-I) envelope protein expressed in insect cells using a recombinant baculovirus was employed as the antigen in an enzyme immunoassay (renvEIA). Peripheral blood samples were obtained from asymptomatic carriers or healthy individuals. Plasma was tested for HTLV-I antibody by renvEIA, particle agglutination, and Western immunoblot (WB), and lymphocyte DNA was tested for HTLV-I proviral DNA amplification by polymerase chain reaction (PCR). Of 61 people aged 9 months or older, 23 were positive (gag+, env+) and 19 others were in the "indeterminate" category (gag+, env-) when their WB results were interpreted according to the WHO-proposed criteria. Thirty-seven cases, including all of the WB+ cases and 14 of 19 WB indeterminate cases, were positive by renvEIA. In 34 of 37 renvEIA-positive cases, the presence of long terminal repeat (LTR) and tax/rex region of HTLV-I proviral DNA was detected by polymerase chain reaction (PCR) and following Southern blot hybridization. Thus, renvEIA would be a useful supplemental assay to confirm the presence of HTLV-I antibody in HTLV-I asymptomatic carriers.
Yamashita
K
K
AIDS Research Center, National Institute of Health, Tokyo, Japan.
Maekawa
M
M
Mitani
K
K
Wakamiya
M
M
Ogino
T
T
Miyamura
K
K
Baba
K
K
Yamamoto
Y
Y
Nyunoya
H
H
Shimotohno
K
K
D000293:Adolescent; D000328:Adult; D000956:Antigens, Viral / Q000276:immunology; D001483:Base Sequence; D002648:Child; D002675:Child, Preschool; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D015686:Gene Products, env / Q000276:immunology*; D015481:HTLV-I Antibodies / Q000032:analysis*; D015490:HTLV-I Infections / Q000175:diagnosis*; D006801:Humans; D007124:Immunoenzyme Techniques / Q000592:standards; D007223:Infant; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D011533:Proviruses / Q000235:genetics; D011994:Recombinant Proteins / Q000276:immunology
D000956:Antigens, Viral; D004279:DNA, Viral; D015686:Gene Products, env; D015481:HTLV-I Antibodies; D011994:Recombinant Proteins