TSH-induced galactose incorporation at the NH2 terminus of thyroglobulin secreted by FRTL-5 cells.
FRTL-5 cells were cultured in media containing standard growth factors with or without TSH, plus labeled precursors of N-linked oligosaccharide chains. The thyroglobulin secreted in the medium was purified and fragmented with CNBr. Three peptides were identified by NH2-terminal sequencing, that were labeled mainly with D-[2-3H]mannose, independent of TSH. One of them, corresponding to the NH2-terminus of thyroglobulin, incorporated both more D-[2-3H]mannose and more D-[1-3H]galactose upon TSH addition. These data likely reflect a TSH-induced increment of N-linked glycosylation at the NH2-terminus of thyroglobulin, mostly with the maturation of high-mannose to complex chains.
Di Jeso
B
B
Centro di Endocrinologia e Oncologia Sperimentale del C.N.R., Naples, Italy.
Gentile
F
F
D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D003488:Cyanogen Bromide; D004591:Electrophoresis, Polyacrylamide Gel; D005690:Galactose / Q000378:metabolism*; D006801:Humans; D008358:Mannose / Q000378:metabolism; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009844:Oligosaccharides / Q000378:metabolism; D010446:Peptide Fragments / Q000302:isolation & purification; D051381:Rats; D013954:Thyroglobulin / Q000302:isolation & purification / Q000378:metabolism*; D013972:Thyrotropin / Q000494:pharmacology*; D014316:Tritium
D009844:Oligosaccharides; D010446:Peptide Fragments; D014316:Tritium; D013972:Thyrotropin; D013954:Thyroglobulin; D003488:Cyanogen Bromide; D008358:Mannose; D005690:Galactose