Comparison of 9-fluorenylmethoxycarbonyl and 9-fluoreneacetyl-tagged silica-based derivatization reagents in high-performance liquid chromatography.
Two silica reagents based on a 4-hydroxy-3-nitrobenzoyl backbone were synthesized and characterized with 9-fluorenylmethoxycarbonyl (FMOC) and 9-fluoreneacetyl (FA) tags. These reagents were tested by derivatization of primary and secondary amines. Derivatization conditions such as temperature, time and triethylamine catalyst were tested. The FA-tagged silica reagent showed better performance than the FMOC-tagged silica reagent by a comparison of derivatization efficiencies, stabilities of reagents, and blank reagent interferences with derivatization. Finally, cadaverine and an aliphatic amine mixture were analysed using the FA-tagged reagent by pre-column, off-line derivatization and fluorescence detection.
Zhang
H M
HM
Department of Chemistry, Northeastern University, Boston, MA 02115.
Zhou
F X
FX
Krull
I S
IS
D000085:Acetates / Q000138:chemical synthesis*; D000588:Amines / Q000032:analysis*; D002103:Cadaverine / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D005449:Fluorenes / Q000138:chemical synthesis*; D007202:Indicators and Reagents / Q000138:chemical synthesis*
D000085:Acetates; D000588:Amines; D005449:Fluorenes; D007202:Indicators and Reagents; C078694:9-fluoreneacetic acid; C054007:1-(9-fluorenyl)methyl chloroformate; D002103:Cadaverine