Use of tRNA consensus primers to indicate subgroups of Pseudomonas solanacearum by polymerase chain reaction amplification.
Polymerase chain reaction amplification of DNA from 112 Pseudomonas solanacearum strains with the tRNA consensus primers T3A and T5A divided the species into three fingerprint groups. These groups correspond well with previous divisions made by restriction fragment length polymorphism analysis. This polymerase chain reaction test is a facile method for rapidly classifying P. solanacearum strains.
Seal
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Sainsbury Laboratory, John Innes Centre, Norwich, Norfolk, United Kingdom.
Jackson
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Daniels
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MJ
D001483:Base Sequence; D016384:Consensus Sequence; D014644:Genetic Variation; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D011549:Pseudomonas / Q000145:classification / Q000235:genetics*; D012343:RNA, Transfer / Q000235:genetics*
D012343:RNA, Transfer