title: Ultrastructural changes in the blood-brain barrier of mice infected with Plasmodium berghei.
abstract: Mice infected with the rodent malaria parasite Plasmodium berghei exhibit ultrastructural changes of the blood-brain barrier during the course of infection. Firm adherence including cellular interdigitation of infected cells or leucocytes and even clusters of cells to the vascular-endothelial lining is repeatedly observed early during infection. Ghosts and membrane remnants can be found engulfed in the surface of the endothelial cells. Frequently leucocytes migrate between endothelial cells and even cause a lift off and degeneration of these cells. In addition, endothelial cells exhibit increased pinocytotic activity, many irregular cytoplasmic extensions and even phagocytic activity. These changes are associated with degenerative changes in the basement membrane. Swelling and deposition of collagen-like fibres and even loss of fragments of basement membrane is observed. In some places fingerlike extensions of pericytes passed through the basement membrane and contacted or even bulged into the cytoplasm of endothelial cells. Ballooning and even coalescence of perivascular astrocytes was observed and contributed to the appearance of a perivascular oedematous space. The observed changes indicate a progressive deterioration of the blood-brain barrier eventually leading to endothelial lesions and hemorrhage.
authors: Department of Neurosurgery, Catholic University of Nijmegen, The Netherlands.
authors: T W
authors: TW
authors: Polder
authors: W M
authors: WM
authors: Eling
authors: J H
authors: JH
authors: Curfs
authors: C R
authors: CR
authors: Jerusalem
authors: M
authors: M
authors: Wijers-Rouw
mesh_terms: D000818:Animals; D001812:Blood-Brain Barrier; D001921:Brain / Q000098:blood supply / Q000648:ultrastructure*; D002543:Cerebral Hemorrhage / Q000473:pathology; D004730:Endothelium, Vascular / Q000648:ultrastructure; D008288:Malaria / Q000473:pathology*; D016779:Malaria, Cerebral / Q000473:pathology*; D051379:Mice; D008810:Mice, Inbred C57BL; D010962:Plasmodium berghei