Presence and coding properties of 2'-O-methyl-5-carbamoylmethyluridine (ncm5Um) in the wobble position of the anticodon of tRNA(Leu) (U*AA) from brewer's yeast.
The unknown modified nucleoside U* has been isolated by enzymatic and HPLC protocols from tRNA(Leu) (U*AA) recently discovered in brewer's yeast. The pure U* nucleoside has been characterized by electron impact mass spectroscopy, and comparison of its chromatographic and UV-absorption properties with those of appropriate synthetic compounds. The structure of U* was established as 2'-O-methyl-5-carbamoylmethyluridine (ncm5Um). The yeast tRNA(Leu) (U*AA) is the only tRNA so far sequenced which has been shown to contain ncm5Um. The location of such a modified uridine at the first position of the anticodon restricts the decoding property to A of the leucine UUA codon.
Glasser
A L
AL
Laboratoire de Biochemie Médicale, Faculté de Médecine, Université de Bourgogne, Dijon, France.
el Adlouni
C
C
Keith
G
G
Sochacka
E
E
Malkiewicz
A
A
Santos
M
M
Tuite
M F
MF
Desgrès
J
J
D000926:Anticodon; D002851:Chromatography, High Pressure Liquid; D005656:Fungal Proteins / Q000096:biosynthesis; D013058:Mass Spectrometry; D015394:Molecular Structure; D012331:RNA, Fungal / Q000235:genetics; D012356:RNA, Transfer, Leu / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D013056:Spectrophotometry, Ultraviolet; D014529:Uridine / Q000031:analogs & derivatives* / Q000032:analysis / Q000737:chemistry / Q000235:genetics
D000926:Anticodon; D005656:Fungal Proteins; D012331:RNA, Fungal; D012356:RNA, Transfer, Leu; C013120:O(2)'-methyl-5-carbamoylmethyluridine; D014529:Uridine