title: A simple and sensitive enzyme-mediated assay of biotin.
abstract: Free biotin was quantitated by a competition by coating biotin-bovine serum albumin conjugate on a polystyrene microplate for binding to avidin-beta-galactosidase conjugate. The enzyme conjugate remaining on the plate surface as a result of the competition was detected by reaction with one of the following fluorogenic substrates, resorufin beta-D-galactoside and fluorescein di-beta-D-galactoside, in a fluorescence plate reader. Free biotin as little as 0.1 nmol can be routinely detected.
authors: Molecular Probes, Inc., Eugene, OR 97402.
authors: Z
authors: Z
authors: Huang
authors: R P
authors: RP
authors: Haugland
authors: D
authors: D
authors: Szalecka
authors: R P
authors: RP
authors: Haugland
mesh_terms: D001360:Avidin; D001667:Binding, Competitive; D001710:Biotin / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D007124:Immunoenzyme Techniques; D013997:Time Factors; D001616:beta-Galactosidase
chemical_list: D001360:Avidin; D001710:Biotin; D001616:beta-Galactosidase