Effect of streptozotocin-induced diabetes on the handling of phosphate by the rat small intestine.
The effect of chemically-induced diabetes on the handling of phosphate (Pi) by rat jejunal enterocytes has been investigated in the presence of a Na- or a choline-gradient. Pi uptake was significantly increased in both gradients. The Pi efflux rate constants for enterocytes from diabetic rats were similar to those of control rats. The effect of diabetes on both the protein and alkaline phosphatase isoenzymes of the rat small intestinal brush-border membranes was examined using SDS-PAGE. The patterns given by membranes from rats 14 days after the induction of diabetes were no different from those of controls.
al-Sawan
S
S
Department of Clinical Biochemistry, Medical School, University of Newcastle upon Tyne, U.K.
Skillen
A W
AW
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001485:Basement Membrane / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D066298:In Vitro Techniques; D017136:Ion Transport; D007527:Isoenzymes / Q000378:metabolism; D007583:Jejunum / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism; D008871:Microvilli / Q000378:metabolism; D010710:Phosphates / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
D007527:Isoenzymes; D008565:Membrane Proteins; D010710:Phosphates; D000469:Alkaline Phosphatase