title: High-performance liquid chromatographic method for quantitating plasma levels of amiloride and its analogues.
abstract: An assay for amiloride was devised for efficient use with the wide variety of analogues available. Amiloride was extracted from 1-ml plasma samples by elution from a C8 preparative column with 6% acetonitrile-45% methanol-5.4% acetic acid, adjusted to pH 4.0 with trimethylamine. Samples were lyophilized, resuspended in 50% methanol, filtered through 0.22-microns Spin-X cartridges, applied to a reversed-phase C18 column, and eluted in a 0-50% acetonitrile gradient in 0.4% acetic acid, pH 4.5 (1.2 ml/min). Detection by ultraviolet absorbance at 360 nm was linear from 1 to 1000 ng. Versatility of the method was demonstrated with the analogues benzamil, 6-hydro-, 6-iodo-, 5-hexamethylene-, and 5-chlorobenzyl-2',4'-dimethylbenzyl-amiloride.
authors: Retina Center, St. Joseph Hospital, Baltimore, MD 21284.
authors: M A
authors: MA
authors: Alliegro
authors: K D
authors: KD
authors: Dyer
authors: E J
authors: EJ
authors: Cragoe
authors: B M
authors: BM
authors: Glaser
authors: M C
authors: MC
authors: Alliegro
mesh_terms: D000284:Administration, Oral; D000584:Amiloride / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000097:blood*; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D011817:Rabbits
chemical_list: C013407:benzamil; D000584:Amiloride