Low temperature induced non-culturability and killing of Vibrio vulnificus.
Vibrio vulnificus cells progressively lose culturability during incubation at 5 degrees C. This process is accelerated by the addition of supernatants from non-culturable cells obtained by incubation at 5 degrees C for 17 days. Thus the organism apparently produces a factor upon cold incubation which is triggering or causing the decline in culturability. Reversing the temperature shift can restore a culturable population comparable in numbers to the original population, but this process is largely due to regrowth. A few cells retaining the ability to grow apparently utilize the substrates released by the moribund cells, thus mimicking resuscitation of the whole population.
Weichart
D
D
Department of General and Marine Microbiology, University of Göteborg, Sweden.
Oliver
J D
JD
Kjelleberg
S
S
D003080:Cold Temperature; D015169:Colony Count, Microbial; D003470:Culture Media; D014733:Vibrio / Q000166:cytology / Q000254:growth & development*
D003470:Culture Media