Lethal effects of the combination of hyperthermia and bromocriptine or a somatostatin analogue on normal pituitary cells and pituitary tumour cells.
Effects of hyperthermia alone and combined with bromocriptine (BC) or a somatostatin analogue (SMS 201-995) were studied in vitro on normal pituitary and pituitary tumour cells (GH3 cells). GH3 cells were more sensitive to heat than normal pituitary cells and a maximal difference in survival was obtained with hyperthermia at 42.5 degrees C for 6 h. BC had cytotoxic and antiproliferative effects on GH3 cells, whereas SMS 201-995 was not effective. Hyperthermia at 42.5 degrees C for 3 h in combination with BC enhanced selective cytotoxicity for GH3 cells. In contrast, combined treatment with hyperthermia and SMS 201-995 failed to enhance cytotoxicity for GH3 cells. The combination of hyperthermia and BC could provide improved therapeutic efficacy in patients with pituitary adenomas.
Kawamoto
K
K
Department of Neurosurgery, Hiroshima University School of Medicine, Japan.
Uozumi
T
T
Yano
T
T
Arita
K
K
Kurisu
K
K
Kiya
K
K
D000818:Animals; D001971:Bromocriptine / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D003131:Combined Modality Therapy; D004247:DNA / Q000096:biosynthesis; D004273:DNA, Neoplasm / Q000096:biosynthesis; D005260:Female; D006358:Hot Temperature; D006979:Hyperthermia, Induced; D066298:In Vitro Techniques; D015282:Octreotide / Q000494:pharmacology*; D010902:Pituitary Gland / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D010911:Pituitary Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000628:therapy*; D051381:Rats; D017208:Rats, Wistar; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000378:metabolism / Q000473:pathology
D004273:DNA, Neoplasm; D001971:Bromocriptine; D004247:DNA; D015282:Octreotide