Comparison of tolerance inducibility to class I or class II antigens between cyclophosphamide (CP)-induced tolerance and transfusion with donor cells: general effectiveness of CP-induced tolerance and difference of skin graft prolongation in each class I antigen-disparate combination.
Transfusion with allogeneic cells alone was reported to prolong skin allograft survival in the MHC class I antigen alone-disparate combination of B6.C-H-2bm1 (bm1; Kbm1, IAb, IE-, Db)-->C57BL/6 CrSlc (B6; H-2b). Using 6 class I antigen-disparate and 2 class II antigen-disparate combinations, we compared the effectiveness for skin graft prolongation between transfusion with donor cells (TDC) and our system of CP-induced tolerance, which comprises intravenous (i.v.) injection of allogeneic cells followed by cyclophosphamide (CP), i.e., TDC followed by CP. TDC was effective only in the combination of bm1-->B6, but not at all in the other combinations. On the other hand, CP-induced tolerance was effective in the 5 class I antigen-disparate and 2 class II antigen-disparate combinations except for bm1-->B6 combination. These results suggest that CP-induced tolerance may be more general for the induction of unresponsiveness than TDC.
Tomita
Y
Y
Division of Cardiovascular Surgery, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Nomoto
K
K
D000818:Animals; D003520:Cyclophosphamide / Q000494:pharmacology*; D005260:Female; D006085:Graft Survival / Q000276:immunology; D015395:Histocompatibility Antigens Class I / Q000276:immunology*; D000949:Histocompatibility Antigens Class II / Q000276:immunology*; D007108:Immune Tolerance / Q000187:drug effects*; D007165:Immunosuppression Therapy / Q000379:methods; D051379:Mice; D008810:Mice, Inbred C57BL; D016038:Skin Transplantation / Q000276:immunology* / Q000379:methods*; D014184:Transplantation, Homologous / Q000276:immunology
D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II; D003520:Cyclophosphamide