Acute effects of a single ethanol injection on in vitro rat uterine spontaneous motility, on uteri prostaglandin production and on tissue metabolism of triglycerides.
The acute effects of ethanol (ETOH), injected at 3 g.kg-1i.p. on spontaneous contractions, on prostaglandin (PG) production and on the metabolism of triglycerides (TGs), have been studied in uterine strips obtained from rats at diestrus and suspended in glucose-containing or glucose-free solution. The absolute values of isometric developed tension (IDT: expressed in mg) recorded at 0 time (initial or post isolation determinations) and the frequency of contraction (FC), expressed as the number of contraction cycles during 20 min, were similar for uterine strips from controls and from ETOH treated rats. The uterine IDT and the FC expressed as percent changes from internal controls (0 time values) were explored during 180 min in uteri suspended in glucose medium. The magnitude of IDT decreased, as time progressed, both in controls as well as in ETOH-treated rats. Afterwards, uterine strips from controls exhibited a partial recovery of their contractile activity. This pattern of recovery was not observed in uterine strips from ETOH-treated rats. The uterine IDT, in the ETOH-injected animals after 180 min of activity, were significantly smaller than those of controls. On the other hand, the FC decreased progressively up to the end of the experimental period both in controls as well as in ETOH-treated rats. In glucose-free medium, the IDT of uterine strips from ETOH-injected animals diminished significantly more than controls from 100-180 min following isolation and mounting. In addition, the FC of uterine strips from the ETOH group of rats were significantly smaller than in controls suspended in glucose-free solution.(ABSTRACT TRUNCATED AT 250 WORDS)
Jaerbaum
A
A
Centro de Estudios Farmacológicos y Botánicos (CEFYBO), Consejo Nacional de Investigaciones Científicas y Técnicas de la República Argentina (CONICET), Buenos Aires.
Chaud
M
M
Gonzalez
E T
ET
Gimeno
A L
AL
Gimeno
M A
MA
D000527:Alprostadil / Q000096:biosynthesis; D000818:Animals; D003470:Culture Media; D015232:Dinoprostone / Q000096:biosynthesis; D000431:Ethanol / Q000633:toxicity*; D005260:Female; D066298:In Vitro Techniques; D011453:Prostaglandins / Q000096:biosynthesis*; D051381:Rats; D017208:Rats, Wistar; D014280:Triglycerides / Q000097:blood / Q000378:metabolism*; D014590:Uterine Contraction / Q000187:drug effects; D014599:Uterus / Q000187:drug effects* / Q000378:metabolism
D003470:Culture Media; D011453:Prostaglandins; D014280:Triglycerides; D000431:Ethanol; D000527:Alprostadil; D015232:Dinoprostone