title: Identification of human TFIID components and direct interaction between a 250-kDa polypeptide and the TATA box-binding protein (TFIID tau).
abstract: Previous studies have indicated that human transcription initiation factor TFIID is a large complex that contains a TATA-binding polypeptide (TFIID tau or TBP) and other components that qualitatively alter promoter interactions and are uniquely required for activator-dependent (versus basal) transcription. TFIID tau-specific antibody columns have been employed to identify a number of human TFIID polypeptides that are tightly associated with TFIID tau. These differ in size from polypeptides in known general initiation factors, including the initiator-binding factor (TFII-I) which shares some promoter binding characteristics with TFIID. The largest component (p250) identified in TFIID was shown to interact directly and tightly with TFIID tau, suggesting that it may play a major role in the assembly of the TFIID complex.
authors: Laboratory of Biochemistry and Molecular Biology, Rockefeller University, New York, NY 10021.
authors: R
authors: R
authors: Takada
authors: Y
authors: Y
authors: Nakatani
authors: A
authors: A
authors: Hoffmann
authors: T
authors: T
authors: Kokubo
authors: S
authors: S
authors: Hasegawa
authors: R G
authors: RG
authors: Roeder
authors: M
authors: M
authors: Horikoshi
mesh_terms: D004268:DNA-Binding Proteins / Q000737:chemistry*; D006801:Humans; D007158:Immunologic Techniques; D046911:Macromolecular Substances; D008970:Molecular Weight; D011485:Protein Binding; D012319:RNA Polymerase II / Q000378:metabolism; D016385:TATA Box; D035181:TATA-Box Binding Protein; D035362:Transcription Factor TFIID; D014157:Transcription Factors / Q000737:chemistry* / Q000276:immunology
chemical_list: D004268:DNA-Binding Proteins; D046911:Macromolecular Substances; D035181:TATA-Box Binding Protein; D035362:Transcription Factor TFIID; D014157:Transcription Factors; D012319:RNA Polymerase II