Application of glycerol to freezing bovine pancreas. Part 3. Effects of freezing rate and glycerol addition on changes in pancreas proteins.
Changes in protein solubility, amino nitrogen and activity of proteases in bovine pancreas frozen at -70 and -18 degrees C after soaking in various aqueous glycerol solutions were studied. Reduction of freezing temperature causes decrease of value in all studied parameters. Additionally, an inhibitory effect of glycerol on protease activity was demonstrated.
Hajduk
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Department of Refrigeration and Food Industry Engineering, Agricultural University, Cracow, Poland.
D000818:Animals; D002417:Cattle; D003451:Cryoprotective Agents / Q000494:pharmacology*; D010450:Endopeptidases / Q000737:chemistry / Q000187:drug effects; D005615:Freezing; D005990:Glycerol / Q000494:pharmacology*; D009584:Nitrogen / Q000032:analysis; D010179:Pancreas / Q000737:chemistry* / Q000187:drug effects* / Q000201:enzymology; D011309:Preservation, Biological; D011506:Proteins / Q000737:chemistry* / Q000187:drug effects*; D012995:Solubility; D013997:Time Factors
D003451:Cryoprotective Agents; D011506:Proteins; D010450:Endopeptidases; D009584:Nitrogen; D005990:Glycerol