Drug transport across the blood-brain barrier. II. Experimental techniques to study drug transport.
This is part II of a review on the transport of drugs across the blood-brain barrier. In this part, the emphasis is on the various experimental techniques that can be used to characterize the blood-brain barrier transport of drugs. Generally speaking, three approaches can be distinguished: in vitro techniques using isolated brain capillaries, cerebrovascular endothelial cells in primary culture or endothelium-derived cell lines; in vivo techniques (both single-passage and multi-passage techniques) and in situ perfusion techniques. Each of these techniques has specific advantages and disadvantages associated with it. Therefore, in many instances, a combination of different approaches is needed to study the fundamental aspects of drug transport across the blood-brain barrier.
Van Bree
J B
JB
Centre for Bio-Pharmaceutical Sciences, Leiden University, Sylvius Laboratories, The Netherlands.
De Boer
A G
AG
Danhof
M
M
Breimer
D D
DD
D000818:Animals; D001692:Biological Transport; D001812:Blood-Brain Barrier; D001921:Brain / Q000098:blood supply / Q000378:metabolism*; D002460:Cell Line; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism; D004912:Erythrocytes / Q000378:metabolism; D006801:Humans; D008433:Mathematics; D004364:Pharmaceutical Preparations / Q000378:metabolism*
D004364:Pharmaceutical Preparations