Purification, crystallization and preliminary X-ray analysis of toxic shock syndrome toxin-1 from Staphylococcus aureus.
High yields of toxic shock syndrome toxin-1, from Staphylococcus aureus, have been purified (> 99%) using a novel, simple, two-step procedure involving dye ligand chromatography. Crystals suitable for X-ray diffraction work were obtained by vapour diffusion using ammonium sulphate and polyethylene glycol as precipitants. They belong to the orthorhombic space group C222(1), with unit cell dimensions a = 108.6 A, b = 177.6 A and c = 97.5 A, with three molecules per asymmetric unit. The crystals diffract to at least 2.5 A resolution and are suitable for three-dimensional X-ray structural analysis.
Passalacqua
E F
EF
Department of Biochemistry, University of Bath, Avon, U.K.
Brehm
R D
RD
Acharya
K R
KR
Tranter
H S
HS
D001427:Bacterial Toxins; D002846:Chromatography, Affinity; D003460:Crystallization; D004768:Enterotoxins / Q000737:chemistry* / Q000302:isolation & purification; D013211:Staphylococcus aureus / Q000737:chemistry*; D018089:Superantigens; D014961:X-Ray Diffraction
D001427:Bacterial Toxins; D004768:Enterotoxins; D018089:Superantigens; C030107:enterotoxin F, Staphylococcal