Evidence for the presence of 5-HT1B receptor messenger RNA in neurons of the rat trigeminal ganglia.
In situ hybridization histochemistry and receptor autoradiography were used to determine the presence of 5-HT1B receptor messenger RNA (mRNA) and 5-HT1B receptor proteins, respectively, in rat trigeminal ganglia. Receptor autoradiography on rat trigeminal ganglia, using appropriate 5-HT1B radioligands, did not demonstrate any specific labelling. In contrast, in situ hybridization showed that many ganglion cells contain 5-HT1B receptor mRNA, suggesting the presence of presynaptic 5-HT1B receptors on trigeminovascular neurons.
Bruinvels
A T
AT
Sandoz Pharma Ltd., Basle, Switzerland.
Landwehrmeyer
B
B
Moskowitz
M A
MA
Hoyer
D
D
D000818:Animals; D001345:Autoradiography; D001665:Binding Sites; D066298:In Vitro Techniques; D007457:Iodine Radioisotopes; D008297:Male; D012333:RNA, Messenger / Q000032:analysis*; D051381:Rats; D017208:Rats, Wistar; D011985:Receptors, Serotonin / Q000235:genetics*; D012668:Trigeminal Ganglion / Q000378:metabolism*
D007457:Iodine Radioisotopes; D012333:RNA, Messenger; D011985:Receptors, Serotonin