title: Characterization of HPV-16 E6/E7 transcription in CaSki cells by quantitative PCR.
abstract: Human papillomavirus (HPV) is associated with specific benign and malignant lesions of the epithelial and mucosal surfaces. Of the sexually transmitted types, HPV type 16 (HPV-16) is the most commonly associated with carcinoma of the uterine cervix. Expression of the E6/E7 open reading frame of the viral genome is considered critical in the development of neoplasia. Using the CaSki cervical carcinoma cell line as a model system, we have adapted the polymerase chain reaction to quantify the transcripts expressed from this region. It was found that 97.1% of the total spliced transcript is E6*I, which putatively encodes the E7 oncoprotein, while E6*II comprises 2.9% of spliced product. The ratio of E6*I to E6*II expression may be an important parameter in evaluating the disease risk associated with HPV-16 infection.
authors: Department of Medical Microbiology, University of Manitoba, Canada.
authors: E M
authors: EM
authors: Hsu
authors: P J
authors: PJ
authors: McNicol
mesh_terms: D001483:Base Sequence; D002277:Carcinoma / Q000382:microbiology* / Q000473:pathology; D015344:DNA Probes, HPV; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D015967:Gene Expression Regulation, Viral; D006801:Humans; D008969:Molecular Sequence Data; D009856:Oncogene Proteins, Viral / Q000096:biosynthesis / Q000235:genetics*; D050725:Papillomavirus E7 Proteins; D016133:Polymerase Chain Reaction; D012326:RNA Splicing; D012334:RNA, Neoplasm / Q000032:analysis*; D012367:RNA, Viral / Q000032:analysis*; D012097:Repressor Proteins; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured / Q000378:metabolism / Q000382:microbiology*; D002583:Uterine Cervical Neoplasms / Q000382:microbiology* / Q000473:pathology
chemical_list: D015344:DNA Probes, HPV; C068651:E6 protein, Human papillomavirus type 16; D009856:Oncogene Proteins, Viral; D050725:Papillomavirus E7 Proteins; D012334:RNA, Neoplasm; D012367:RNA, Viral; D012097:Repressor Proteins; C059731:oncogene protein E7, Human papillomavirus type 16