Solid-phase synthesis of branched oligoribonucleotides related to messenger RNA splicing intermediates.
The chemical synthesis of oligoribonucleotides containing vicinal (2'-5')- and (3'-5')-phosphodiester linkages is described. The solid-phase method, based on silyl-phosphoramidite chemistry, was applied to the synthesis of a series of branched RNA [(Xp)nA2' (pN)n3'(pN)n] related to the splicing intermediates derived from Saccharomyces cerevisiae rp51a pre-messenger RNA. The branched oligonucleotides have been thoroughly characterized by nucleoside and branched nucleotide composition analysis. Branched oligoribonucleotides will be useful in the study of messenger RNA splicing and in determining the biological role of RNA 'lariats' and 'forks' in vivo.
Damha
M J
MJ
Department of Chemistry, Erindale College, University of Toronto, Ontario, Canada.
Ganeshan
K
K
Hudson
R H
RH
Zabarylo
S V
SV
D001483:Base Sequence; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D007202:Indicators and Reagents; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009843:Oligoribonucleotides / Q000138:chemical synthesis* / Q000737:chemistry / Q000302:isolation & purification; D012322:RNA Precursors / Q000138:chemical synthesis / Q000737:chemistry; D012326:RNA Splicing; D012333:RNA, Messenger / Q000138:chemical synthesis*
D007202:Indicators and Reagents; D009843:Oligoribonucleotides; D012322:RNA Precursors; D012333:RNA, Messenger