title: Thiamine secretion in yeast.
abstract: To isolate thiamine excretors and (or) overproducers, 188 cultures belonging to nine yeast and three fungal genera were screened. Nine excreted thiamine as determined by both the presence of cross-feeding zones on a thiamine-free agar medium seeded with a thiamine-requiring yeast strain and by the direct detection of excreted thiamine on agar plates. Several of these cultures produced several-fold more intracellular thiamine than the general culture population. Thiamine-requiring strains of Saccharomyces cerevisiae and S. uvarum (carlsbergensis) were identified and were tentatively assigned to 10 complementation groups.
authors: Biology Department, Brock University, St. Catharines, Ont., Canada.
authors: Y
authors: Y
authors: Haj-Ahmad
authors: C A
authors: CA
authors: Bilinski
authors: I
authors: I
authors: Russell
authors: G G
authors: GG
authors: Stewart
mesh_terms: D005110:Extracellular Space / Q000378:metabolism; D005658:Fungi / Q000378:metabolism; D005816:Genetic Complementation Test; D007424:Intracellular Fluid / Q000378:metabolism; D012440:Saccharomyces / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D013045:Species Specificity; D013831:Thiamine / Q000378:metabolism*; D015003:Yeasts / Q000235:genetics / Q000378:metabolism*
chemical_list: D013831:Thiamine