[The use of tapioca as coverage in viral titration].
Virus titration is an important step required on viral vaccines quality control. "Plaque assay", which employs several types of overlay media, is usually used on viral titrations. In this paper we describe the use of Tapioca as an overlay media. Firstly, the toxicity of Tapioca was tested on Vero cells inoculated or not with the Yellow Fever virus (YF) 17DD vaccine strain. Secondly, different batches of the 17DD virus using the Tapioca and Karaya gum as the overlay on Vero cells were tested when higher titres were obtained using Tapioca. Tapioca was also shown to be a suitable overlay to be used in thermostability and plaque reduction neutralization tests. Other systems could benefit from the use of Tapioca as an overlay, since it was possible to titer Measles virus in Vero cells. Tapioca is a cheap Brazilian product, is locally available, easy to use, and reliable. Its use is suggested.
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Departamento de Bioquímica e Biologia Molecular, Instituto de Tecnologia em Imunobiológicos, Rio de Janeiro, Brasil.
de Carvalho
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Brito
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Galler
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D000818:Animals; D007618:Karaya Gum; D008253:Macaca mulatta; D002365:Manihot; D008459:Measles virus / Q000254:growth & development; D009500:Neutralization Tests; D015203:Reproducibility of Results; D014709:Vero Cells; D010948:Viral Plaque Assay / Q000379:methods*; D015005:Yellow fever virus / Q000254:growth & development*
D007618:Karaya Gum
O uso da tapioca como cobertura na titulação viral.