[The identification of the sites of tRNA(Ser)(GCU) interaction in bovine liver with homologous aminoacyl-tRNA-synthetase by the chemical modification method].
Interaction of the bovine liver tRNA(GCUSer) having a long variable loop, with the cognate aminoacyl-tRNA synthetase has been studied by alkylation with ethylnitrosourea. It was shown that seryl-tRNA synthetase protects 3'-phosphates of nucleotides 12, 13 in D-stem and 45-47-, 47 G.-, 47 H-variable stem of tRNA(GCUreS) from alkylation. An anticodon loop of tRNA(GCUSer) did not interact with seryl-tRNA synthetase.
Kalachynok
L H
LH
Tukalo
M A
MA
Matsuka
H Kh
HKh
D000478:Alkylation; D000604:Amino Acyl-tRNA Synthetases / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D001665:Binding Sites; D002417:Cattle; D004347:Drug Interactions; D005038:Ethylnitrosourea / Q000494:pharmacology; D066298:In Vitro Techniques; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D012362:RNA, Transfer, Ser / Q000187:drug effects / Q000302:isolation & purification / Q000378:metabolism*
D012362:RNA, Transfer, Ser; D000604:Amino Acyl-tRNA Synthetases; D005038:Ethylnitrosourea
Vyznachennia dilianok vzaiemodiï tRNK(Ser)(GCU) pechinky byka iz homol'ohichnoiu aminoatsyl-tRNK-syntetazoiu metodom khemichnoï modyfikatsiï.