title: Changes in plasma membrane protein composition during early development of the frog Rana ridibunda.
abstract: Plasma membranes of fully-grown oocytes and early developmental stage embryos of Rana ridibunda were isolated by differential and density gradient centrifugation; they were purified 18-fold as indicated by 5'-nucleotidase. The plasma membrane protein pattern of five different developmental stages was studied by two-dimensional polyacrylamide gel electrophoresis. More than 60 protein species could be detected by silver staining. Most of them are largely conserved during development. The rest either show precise stage specificity or exhibit stronger staining intensity at particular stages. The number of proteins specific for each stage is small, neurula being exempted. The changes observed in the plasma membrane profile during development are mostly prominent in a group of proteins with similar molecular weights (40-45 kDa) but with different pI values. The differences observed in the plasma membrane patterns are discussed in relation to the significance of each stage during development.
authors: Dept. of Genetics, Developmental and Molecular Biology, University of Thessaloniki, Greece.
authors: A
authors: A
authors: Beis
authors: A
authors: A
authors: Lazou
authors: E
authors: E
authors: Kontogianni
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000737:chemistry; D002458:Cell Fractionation; D002462:Cell Membrane / Q000737:chemistry / Q000648:ultrastructure; D002499:Centrifugation, Density Gradient; D015180:Electrophoresis, Gel, Two-Dimensional; D004625:Embryo, Nonmammalian / Q000737:chemistry / Q000502:physiology*; D005314:Embryonic and Fetal Development; D005775:Gastrula / Q000737:chemistry; D007525:Isoelectric Focusing; D008565:Membrane Proteins / Q000032:analysis / Q000378:metabolism*; D008970:Molecular Weight; D009865:Oocytes / Q000737:chemistry / Q000502:physiology*; D011895:Rana ridibunda
chemical_list: D008565:Membrane Proteins