title: Simultaneous synthesis of degenerate oligonucleotides of variable length.
abstract: Most mutagenic studies have emphasized the exchange of residues but disregarded the variation in length as the other aspect of protein variability. In this report a novel strategy to simultaneously synthesize degenerate mutagenic oligonucleotides of variable length is reported. Synthesis is done on a normal automated DNA synthesizer with modifications only in the program. Product of such synthesis can be used as a mutagenic oligonucleotide for construction of mutant proteins with variable length of inserts.
authors: Dept. of Biochemistry, Jozef Stefan Institute, Ljublijana, Slovenia.
authors: R
authors: R
authors: Jerala
mesh_terms: D001483:Base Sequence; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000138:chemical synthesis*; D013777:Tetrazoles
chemical_list: D009841:Oligonucleotides; D013777:Tetrazoles; C045574:1H-tetrazole