Evidence for the presence and location of annexins in human platelets.
In this study the identity of annexins in human platelets has been determined together with their ability to be released by agents which induce platelet degranulation. The presence of proteins cross-reacting to antibodies against annexins I and V was detected in human platelets. However, the study provided evidence that these annexins are not located on the surface of the plasma membrane in a Ca++ dependent manner. Moreover, activation of platelets with several agents which induced platelet degranulation did not cause release of annexins I or V as determined by both immunoblotting and ELISA.
Murphy
C T
CT
Department of Pharmacology, University of Bath, Avon, UK.
Peers
S H
SH
Forder
R A
RA
Flower
R J
RJ
Carey
F
F
Westwick
J
J
D017305:Annexin A1 / Q000097:blood; D017306:Annexin A2 / Q000097:blood; D017314:Annexin A4 / Q000097:blood; D017304:Annexin A5 / Q000097:blood; D017302:Annexins / Q000097:blood*; D000906:Antibodies; D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism*; D015153:Blotting, Western; D003429:Cross Reactions; D003594:Cytoplasmic Granules / Q000378:metabolism; D004492:Edetic Acid / Q000494:pharmacology; D004533:Egtazic Acid / Q000494:pharmacology; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D015759:Ionomycin / Q000494:pharmacology; D007700:Kinetics; D010972:Platelet Activating Factor / Q000494:pharmacology; D015539:Platelet Activation; D012701:Serotonin / Q000097:blood; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D013917:Thrombin / Q000494:pharmacology
D017305:Annexin A1; D017306:Annexin A2; D017314:Annexin A4; D017304:Annexin A5; D017302:Annexins; D000906:Antibodies; D010972:Platelet Activating Factor; D012701:Serotonin; D004533:Egtazic Acid; D015759:Ionomycin; D004492:Edetic Acid; D013917:Thrombin; D013755:Tetradecanoylphorbol Acetate