Characterization of chlorophyll a/b proteins of photosystem I from Chlamydomonas reinhardtii.
In this study we have isolated the chlorophyll a/b-binding proteins from a photosystem I preparation of the green alga Chlamydomonas reinhardtii and characterized them by N-terminal sequencing, fluorescence, and absorption spectroscopy and by immunochemical means. The results indicate that in this organism, the light-harvesting complex of photosystem I (LHCI) is composed of at least seven distinct polypeptides of which a minimum number of three are shown to bind chlorophyll a and b. Both sequence homology and immunological cross-reactivity with other chlorophyll-binding proteins suggest that all of the LHCI polypeptides bind pigments. Fractionation of LHCI by mildly denaturing methods showed that, in contrast to higher plants, the long wavelength fluorescence emission typical of LHCI (705 nm in C. reinhardtii) cannot be correlated with the presence of specific polypeptides, but rather with changes in the aggregation state of the LHCI components. Reconstitution of both high aggregation state and long wavelength fluorescence emission from components that do not show these characteristics confirm this hypothesis.
Bassi
R
R
Dipartimento di Biologia, Università di Padova, Italy.
Soen
S Y
SY
Frank
G
G
Zuber
H
H
Rochaix
J D
JD
D000595:Amino Acid Sequence; D000818:Animals; D002338:Carotenoids / Q000032:analysis; D002499:Centrifugation, Density Gradient; D016825:Chlamydomonas reinhardtii / Q000378:metabolism*; D002734:Chlorophyll / Q000032:analysis; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D007425:Intracellular Membranes / Q000378:metabolism; D007700:Kinetics; D045342:Light-Harvesting Protein Complexes; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D008970:Molecular Weight; D045322:Photosynthetic Reaction Center Complex Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D045331:Photosystem I Protein Complex; D011485:Protein Binding; D017386:Sequence Homology, Amino Acid; D013053:Spectrophotometry
D045342:Light-Harvesting Protein Complexes; D046911:Macromolecular Substances; D045322:Photosynthetic Reaction Center Complex Proteins; D045331:Photosystem I Protein Complex; D002734:Chlorophyll; D002338:Carotenoids