The 5S ribosomal RNA gene is linked to large and small subunit ribosomal RNA genes in the oomycetes, Phytophthora vignae, P. cinnamomi, P. megasperma f.sp. glycinea and Saprolegnia ferax.
Southern hybridization and polymerase chain reaction data indicate that the 5S ribosomal RNA gene is linked to the ribosomal RNA gene repeat unit in the oomycetes, Phytophthora vignae, P. cinnamomi, P. megasperma f.sp. glycinea and Saprolegnia ferax, and is apparently transcribed in the same direction as the large and small subunit ribosomal RNA genes. The polymerase chain reaction has been used to amplify all components of the entire ribosomal RNA gene repeat unit for each of these oomycetes. The total size of all amplified products is identical to the size of the ribosomal RNA gene repeat unit, as determined by Southern analysis.
Howlett
B J
BJ
School of Botany, University of Melbourne, Parkville, Victoria, Australia.
Brownlee
A G
AG
Guest
D I
DI
Adcock
G J
GJ
McFadden
G I
GI
D001483:Base Sequence; D015139:Blotting, Southern; D004275:DNA, Ribosomal / Q000235:genetics; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D009841:Oligonucleotides; D009868:Oomycetes / Q000235:genetics*; D010838:Phytophthora / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012331:RNA, Fungal / Q000235:genetics; D012335:RNA, Ribosomal / Q000235:genetics*; D012341:RNA, Ribosomal, 5S / Q000235:genetics*
D004275:DNA, Ribosomal; D009841:Oligonucleotides; D012331:RNA, Fungal; D012335:RNA, Ribosomal; D012341:RNA, Ribosomal, 5S