Simulation of maternal immunity by inoculation of immune yolk preparations into the yolk sac of 1-day-old chickens.
Yolk harvested from eggs laid by hens hyperimmunized with killed Newcastle disease virus (NDV) was inoculated into the yolk sac of 1-day-old specific-pathogen-free (SPF) chickens. Serum hemagglutination-inhibition antibody titers reached maximum levels 1 to 4 days after yolk inoculation and declined at a rate similar to that reported for naturally acquired maternal antibody. Expected levels of immune interference were observed when yolk-inoculated chickens were vaccinated with a conventional oil-emulsion NDV vaccine. These results show that yolk-sac inoculation with yolk antibody is a suitable approach for producing maternally immune chickens for laboratory studies.
Stone
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HD
U.S. Department of Agriculture, Southeast Poultry Research Laboratory, Athens, Georgia 30605.
Brugh
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M
Xie
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D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D002645:Chickens / Q000276:immunology*; D004530:Egg Yolk / Q000276:immunology*; D006385:Hemagglutination Inhibition Tests / Q000662:veterinary; D007112:Immunity, Maternally-Acquired; D007116:Immunization, Passive / Q000379:methods / Q000662:veterinary*; D009522:Newcastle disease virus / Q000276:immunology; D014765:Viral Vaccines / Q000276:immunology
D000914:Antibodies, Viral; D014765:Viral Vaccines