Fluorescence in situ hybridization to determine engraftment status after murine bone marrow transplant.
The mouse Y-specific DNA sequence pY2 was used as a probe for fluorescence in situ hybridization (FISH) to evaluate murine hematopoietic tissues after sex-mismatched bone marrow transplant (BMT). The pY2 probe was localized to the long arm of the Y chromosome on BM metaphases. Hybridization of pY2 in FISH of interphase cells from BM, spleen, and thymus after BMT was compared with Southern blot analysis; both methods gave comparable results. Only FISH was able to analyze post-BMT peripheral blood (PB) samples successfully, and provides a useful method for following engraftment status in the mouse on an ongoing basis.
Hawkins
A L
AL
Johns Hopkins Oncology Center, Baltimore, MD 21205.
Jones
R J
RJ
Zehnbauer
B A
BA
Zicha
M S
MS
Collector
M J
MJ
Sharkis
S J
SJ
Griffin
C A
CA
D000818:Animals; D015139:Blotting, Southern; D001854:Bone Marrow Cells; D016026:Bone Marrow Transplantation; D004247:DNA / Q000032:analysis; D015342:DNA Probes; D005260:Female; D005819:Genetic Markers; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D008297:Male; D051379:Mice; D014185:Transplantation, Isogeneic; D014998:Y Chromosome
D015342:DNA Probes; D005819:Genetic Markers; D004247:DNA