Monoclonal antibody BRL 12 reacts with bone keratan sulphate proteoglycan.
Our previous studies suggest that a monoclonal antibody, BRL 12, reacts with a specific product of differentiated cells of the osteoblastic lineage. In the present study, the bone constituent recognized by this antibody has been characterized by gel filtration, ion exchange chromatography, protein blotting and immunolocalization. Our findings show that the antibody reacts with an epitope associated with the core protein of rabbit keratan sulfate proteoglycan (KSPG), a molecule which shares considerable homology with the sialoprotein present in the bone tissue of other mammalian species.
Joyner
C J
CJ
Nuffield Department of Orthopaedic Surgery, University of Oxford, England.
Virdi
A S
AS
Beresford
J N
JN
Bennett
J H
JH
Owen
M E
ME
Triffitt
J T
JT
D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry*; D000937:Antigen-Antibody Reactions; D001666:Binding Sites, Antibody; D001842:Bone and Bones / Q000737:chemistry / Q000276:immunology*; D003315:Cornea / Q000737:chemistry / Q000276:immunology; D007632:Keratan Sulfate / Q000737:chemistry / Q000276:immunology*; D011817:Rabbits
D000911:Antibodies, Monoclonal; D007632:Keratan Sulfate