title: Morphological effects of photodynamic therapy on rabbit bladder using Photofrin II and Photosan intravesically and intravenously.
abstract: Photodynamic therapy (PDT) has proved effective against superficial papillary bladder tumours and focal and diffuse carcinoma in situ. Effective topical administration of the sensitiser would be a welcome improvement. The morphological effects of PDT on the normal bladder were examined in 13 rabbits when 2 photosensitisers (Photofrin II and Photosan III) were applied intravesically (5 mg/kg for 1 h) and compared with intravenous administration (3 or 5 mg/kg). Four animals served as controls without a sensitiser. Intravesical red light (630 nm) from an argon dye laser was used to activate the photosensitiser, using light doses of 12 or 24 J/cm2. The animals were sacrificed either 1 or 5 to 7 days after the laser treatment. Intravenous dosage induced bladder wall oedema/haemorrhage and total necrosis of the epithelium. There was no difference between the effects of the 2 sensitisers. Intravesical application induced superficial epithelial necrosis. The control animals treated with laser light alone showed slight superficial injury to the cell layer.
authors: Department of Urology, Helsinki University Central Hospital, Finland.
authors: K
authors: K
authors: Taari
authors: M
authors: M
authors: Talja
authors: M
authors: M
authors: Riihelä
authors: S
authors: S
authors: Rannikko
authors: R
authors: R
authors: Mokka
mesh_terms: D000283:Administration, Intravesical; D000818:Animals; D017323:Dihematoporphyrin Ether / Q000008:administration & dosage / Q000009:adverse effects*; D004848:Epithelium / Q000648:ultrastructure; D006415:Hematoporphyrins; D008297:Male; D008855:Microscopy, Electron, Scanning; D009336:Necrosis / Q000139:chemically induced; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000008:administration & dosage / Q000009:adverse effects*; D011817:Rabbits; D001743:Urinary Bladder / Q000187:drug effects* / Q000648:ultrastructure
chemical_list: D006415:Hematoporphyrins; D017319:Photosensitizing Agents; C071875:photosan III; D017323:Dihematoporphyrin Ether