Gas chromatographic-mass spectrometric determination of plasma selegiline using a deuterated internal standard.
A gas chromatographic-mass spectrometric method is described for the determination of plasma selegiline. Tetradeuteroselegiline was synthesized and served as the internal standard. Human plasma samples (1 ml) containing 1-6 ng of selegiline were acidified, washed with diethyl ether-hexane, then alkalinized and extracted with heptane-isoamyl alcohol. Analytical separations were performed on a dimethylsilicone capillary column. Detection was by selected ion monitoring of the electron impact generated m/z 96 and 100 alpha-cleavage fragments of drug and internal standard, respectively.
Patrick
K S
KS
Department of Pharmaceutical Sciences, Medical University of South Carolina, Charleston 29425.
Nguyen
B L
BL
McCallister
J D
JD
D003903:Deuterium; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D015203:Reproducibility of Results; D012642:Selegiline / Q000097:blood*
D012642:Selegiline; D003903:Deuterium