Gentamicin, norfloxacin and lysozyme concentration in human tears: in vivo and in vitro study.
Hen's egg lysozyme (HEL) activity was measured in vitro with gentamicin and norfloxacin by a turbidimetric technique. Gentamicin at the concentration of 10(-3) M inhibited HEL activity by 39%, while 10(-3) M norfloxacin did not affect HEL activity. However, an in vivo study in healthy persons did not show any significant statistical difference in tear lysozyme activity when 0.3% gentamicin or 0.3% norfloxacin were topically applied.
Bron
A M
AM
Department of Ophthalmology, University of Burgundy, Dijon, France.
Bonnin
C
C
Garcher
C
C
Lallemand
A
A
D000293:Adolescent; D000328:Adult; D003315:Cornea / Q000187:drug effects; D005839:Gentamicins / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D009113:Muramidase / Q000187:drug effects / Q000378:metabolism*; D009643:Norfloxacin / Q000494:pharmacology*; D009883:Ophthalmic Solutions; D013666:Tears / Q000187:drug effects / Q000201:enzymology*
D005839:Gentamicins; D009883:Ophthalmic Solutions; D009113:Muramidase; D009643:Norfloxacin