Enhanced acetylcholine secretion in neuroblastoma x glioma hybrid NG108-15 cells transfected with rat choline acetyltransferase cDNA.
Neuroblastoma x glioma hybrid NG108-15 cells and mouse neuroblastoma N18TG-2 and N1E-115 cells were transiently transfected with the sense cDNA coding for rat choline acetyltransferase (ChAT). All transfected cell lines showed a high level of ChAT activity. ACh secretion was monitored by recording miniature end-plate potentials (MEPPs) in striated muscle cells that had been co-cultured with transfected cells. The number of muscle cells with synaptic responses and the MEPP frequency were higher in co-culture with transfected NG108-15 cells than with control or mock cells. No synaptic response was detected in muscle cells co-cultured with transfected N18TG-2 or N1E-115 cells. The results show that ACh secretion into the synaptic cleft was enhanced due to ChAT overexpression in NG108-15 hybrid cells but not in neuroblastoma cells.
Kimura
Y
Y
Department of Biophysics, Kanazawa University School of Medicine, Japan.
Oda
Y
Y
Deguchi
T
T
Higashida
H
H
D000109:Acetylcholine / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D002478:Cells, Cultured; D002795:Choline O-Acetyltransferase / Q000235:genetics* / Q000378:metabolism; D005910:Glioma; D006822:Hybrid Cells; D007700:Kinetics; D051379:Mice; D009132:Muscles / Q000166:cytology; D009447:Neuroblastoma; D009474:Neurons / Q000378:metabolism*; D051381:Rats; D013569:Synapses; D014162:Transfection; D014407:Tumor Cells, Cultured
D002795:Choline O-Acetyltransferase; D000109:Acetylcholine