A nuclear magnetic resonance imaging technique designed for studies of water in plant leaves.
A new imaging technique is described which uses nuclear magnetic resonance (NMR) to create a water profile of plant leaves. The water profile shows the average distribution of water as a function of depth into a leaf along a line perpendicular to the leaf surface; it can be used to measure the thickness of cell layers and the quantity of water in each layer. Two-dimensional NMR methods were used to avoid chemical shift distortion which degrades the resolution in leaf images made by conventional NMR techniques; image resolution was improved further by deconvolution analysis. To illustrate its application, the technique was used to follow changes in the internal structure of developing leaves.
McCain
D C
DC
Department of Chemistry and Biochemistry, University of Southern Mississippi, Hattiesburg 39406.
Markley
J L
JL
D005069:Evaluation Studies as Topic; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D063245:Plant Development; D010944:Plants / Q000033:anatomy & histology / Q000378:metabolism*; D014867:Water / Q000737:chemistry / Q000378:metabolism*
D014867:Water