title: S-antigen localization in developing rds mouse retina.
abstract: The morphology of the photoreceptor cell and localization of the cytoplasmic soluble protein, S-antigen, in the retina of the developing retinal degeneration slow (rds) mutant mouse (2-505 postnatal days) were studied by improved immunocytochemical and freeze-substitution methods. Anti-S-antigen antibody labeling was observed first in the postnatal 10-day retina under light microscope. Labeling signals increased progressively to a maximum level in 20 days, and then decreased gradually to an undetectable level by 505 postnatal days. By electron microscopic immunocytochemical methods, S-antigen was detected first in the photoreceptor cells at 3 postnatal days, and increased with development. It was located in the entire cytoplasm of the photoreceptor cell including the rudimentary outer segment, but degenerated and disappeared by 505 postnatal days. S-antigen was also present in the membranous vesicles budding off from the photoreceptor membrane to the subretinal space. The rds photoreceptor cell seems to lose other soluble proteins together with these vesicles. From these results and other published data, we speculate that the degeneration of the photoreceptor cells may be the secondary effects of the loss of a large amount of soluble and membrane proteins following the malfunction of membrane. Recent reports show the rds mouse must have a gene defect in the membrane component such as peripherin or the 39 kDa protein.
authors: Department of Ophthalmology, Nagoya University, Japan.
authors: S
authors: S
authors: Sai
authors: J
authors: J
authors: Usukura
authors: T
authors: T
authors: Shinohara
authors: T
authors: T
authors: Wakabayashi
authors: S
authors: S
authors: Awaya
mesh_terms: D000818:Animals; D000941:Antigens / Q000378:metabolism*; D019393:Arrestin; D005136:Eye Proteins / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008817:Mice, Mutant Strains; D010726:Phosphodiesterase Inhibitors / Q000378:metabolism*; D010786:Photoreceptor Cells / Q000378:metabolism; D012160:Retina / Q000254:growth & development / Q000378:metabolism* / Q000648:ultrastructure; D012162:Retinal Degeneration / Q000378:metabolism* / Q000473:pathology
chemical_list: D000941:Antigens; D019393:Arrestin; D005136:Eye Proteins; D008565:Membrane Proteins; D010726:Phosphodiesterase Inhibitors