title: Binding of human IgA to HCl-extracted c protein from group B streptococci (GBS).
abstract: The c beta protein of group B streptococci obtained by HCl extraction appears as a ladder-like pattern in SDS-PAGE when detected by a rabbit anti-c beta serum, and a similar picture is seen when the crude extract is incubated with human IgA and an anti-human IgA conjugate. Affinity-purified c beta antigen and IgA receptors from GBS gave identical pictures in Western blots using rabbit anti-c beta serum. Both the c beta antigen and the IgA receptor are exposed on the surface of GBS as demonstrated by immunofluorescence.
authors: Department of Microbiology, Faculty of Medicine, Regional Hospital, University of Trondheim, Norway.
authors: A I
authors: AI
authors: Kvam
authors: O J
authors: OJ
authors: Iversen
authors: L
authors: L
authors: Bevanger
mesh_terms: D000942:Antigens, Bacterial / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D006801:Humans; D006851:Hydrochloric Acid; D007106:Immune Sera / Q000276:immunology; D015151:Immunoblotting; D007070:Immunoglobulin A / Q000378:metabolism*; D011961:Receptors, Fc; D011971:Receptors, Immunologic / Q000032:analysis; D013292:Streptococcus agalactiae / Q000378:metabolism*
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; C045281:IgA receptor; D007106:Immune Sera; D007070:Immunoglobulin A; D011961:Receptors, Fc; D011971:Receptors, Immunologic; C070010:streptococcal beta antigen; D006851:Hydrochloric Acid