Comparative study of some factors affecting enumeration of moulds using dilution plate techniques.
The influence of dilution plating technique, nature of diluent, culture media and incubation period on the enumeration of moulds have been studied. Three new culture media containing Auramine, Gentian Violet and Malachite Green respectively have been induced in this study. No significant differences were observed between results obtained after 3, 5 and 7 days of incubation. Significantly higher recoveries were obtained using the surface-spread method than pour plate method. Using the first technique no effect of diluent was observed, and among the different culture media studied higher counts were obtained with medium containing Auramine.
Bragulat
M R
MR
Departamento de Patología y Producción Animales (Microbiología), Facultad de Veterinaria, Universidad Autónoma de Barcelona, Spain.
Abarca
M L
ML
Bruguera
M T
MT
Cabañes
F J
FJ
D001576:Benzophenoneidum; D003470:Culture Media; D005516:Food Microbiology; D005658:Fungi / Q000302:isolation & purification*; D005840:Gentian Violet; D007201:Indicator Dilution Techniques; D008828:Microbiological Techniques; D012394:Rosaniline Dyes; D013172:Spores, Fungal
D003470:Culture Media; D012394:Rosaniline Dyes; C005095:malachite green; D001576:Benzophenoneidum; D005840:Gentian Violet