Development of enzyme-linked immunosorbent assays for conglutinin, mannan-binding protein, and serum amyloid-P component in bovine sera.
Sandwich ELISA were developed to quantitatively determine conglutinin (CG), mannan-binding protein (MBP), and serum amyloid-P component (SAP) in the sera of cattle. The ELISA system was found to have high repeatability for quantitation of these serum proteins at concentration as low as 5 ng/ml. From results obtained for 10 healthy cows aged 2 to 7 years, mean +/- SD serum concentrations were 56.5 +/- 14.4 micrograms of CG/ml, 2.37 +/- 0.87 micrograms of MBP/ml, and 11.14 +/- 3.92 micrograms of SAP/ml, respectively. Values in 6 healthy heifer calves aged 6 months were 3.45 +/- 1.22 micrograms/ml for CG, 1.71 +/- 0.96 micrograms/ml for MBP, and 5.45 +/- 2.75 micrograms/ml for SAP, respectively. Concentrations in 9 healthy bullocks aged 6 months were 1.83 +/- 0.66 micrograms/ml for CG, 1.04 +/- 0.63 micrograms/ml for MBP, and 4.9 +/- 1.13 micrograms/ml for SAP, respectively.
Akiyama
K
K
Ashoro Branch, Hokkaido Tokachi Agricultural Mutual-Aid Association, Japan.
Sugii
S
S
Hirota
Y
Y
D000818:Animals; D002352:Carrier Proteins / Q000097:blood*; D002417:Cattle / Q000097:blood*; D037222:Collectins; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D005260:Female; D008297:Male; D012016:Reference Values; D015203:Reproducibility of Results; D000683:Serum Amyloid P-Component / Q000032:analysis*; D012712:Serum Globulins / Q000032:analysis*
D002352:Carrier Proteins; D037222:Collectins; D000683:Serum Amyloid P-Component; D012712:Serum Globulins; C024014:conglutinin