Effects of Guillain-Barré sera containing antibodies against glycolipids in cultures of rat Schwann cells and sensory neurons.
Serum samples from 52 patients with the acute Guillain-Barré syndrome (GBS), 19 patients with other neurological disorders, and 18 healthy volunteers were tested for cytotoxicity in cultures of rat Schwann cells and dorsal root ganglion neurons. The samples were also examined by enzyme-linked immunosorbent assay for IgG and IgM antibodies against various acidic and neutral glycolipids. Samples from 16 of the 52 (31%) acute GBS patients and from 1 of the 6 patients with chronic inflammatory demyelinating polyneuropathy produced myelin breakdown in culture. Although 10 of the 16 cytotoxic acute GBS serum samples contained anti-glycolipid immunoglobulins, there was no correlation in individual samples between cytotoxic activity and the presence of antibodies against specific glycolipids. While our results do not exclude a role for anti-glycolipid antibodies in the pathogenesis of the acute GBS, the cytotoxic effects of acute GBS serum in cultures of Schwann cells and sensory neurons are probably not due to these antibodies alone.
Mithen
F A
FA
John Cochran VA Medical Center, St. Louis, MO.
Ilyas
A A
AA
Birchem
R
R
Cook
S D
SD
D000818:Animals; D000906:Antibodies / Q000276:immunology*; D003601:Cytotoxicity Tests, Immunologic; D004797:Enzyme-Linked Immunosorbent Assay; D004912:Erythrocytes / Q000737:chemistry / Q000378:metabolism; D005260:Female; D005915:Globosides / Q000276:immunology; D006017:Glycolipids / Q000276:immunology*; D006801:Humans; D008854:Microscopy, Electron; D009475:Neurons, Afferent / Q000276:immunology*; D011129:Polyradiculoneuropathy / Q000276:immunology*; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley; D012583:Schwann Cells / Q000276:immunology*
D000906:Antibodies; D005915:Globosides; D006017:Glycolipids; C014812:sialosylparagloboside