title: Palmitate oxidation by in vivo and in vitro grown Mycobacterium lepraemurium.
abstract: Oxidation of palmitate by Mycobacterium lepraemurium isolated from C3H mice lepromata (in vivo) and also grown on Ogawa egg-yolk medium (in vitro) was investigated. Palmitate was found to be oxidized, after a lag period of about 8 h, by both the in vivo and in vitro grown bacilli. Cell-free extracts prepared from in vivo and in vitro grown cells catalysed an active oxidation of palmitate after a lag period of 3-4 h. The amount of ATP increased, with the increase in time during oxidation of palmitate by the cell-free extracts. The generation of ATP was strongly inhibited by the inhibitors rotenone, antimycin A and cyanide as well as by the uncouplers 2,4-dinitrophenol and 2,6-dibromophenol. These results indicated that oxidation of palmitate by the in vivo and in vitro grown M. lepraemurium is mediated through the respiratory chain using oxygen as the terminal electron acceptor.
authors: Centre de Recherche en Microbiologie Appliquée, Institut Armand-Frappier, Université du Québec, Laval-des-Rapides, Canada.
authors: M
authors: M
authors: Ishaque
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D000968:Antimycin A / Q000494:pharmacology; D002474:Cell-Free System / Q000378:metabolism; D003486:Cyanides / Q000494:pharmacology; D005260:Female; D051379:Mice; D008809:Mice, Inbred C3H; D009167:Mycobacterium lepraemurium / Q000187:drug effects / Q000378:metabolism*; D010084:Oxidation-Reduction; D010100:Oxygen / Q000378:metabolism; D019308:Palmitic Acid; D010169:Palmitic Acids / Q000378:metabolism*; D012119:Respiration; D012402:Rotenone / Q000494:pharmacology
chemical_list: D003486:Cyanides; D010169:Palmitic Acids; D012402:Rotenone; D019308:Palmitic Acid; D000968:Antimycin A; D000255:Adenosine Triphosphate; D010100:Oxygen