Characterization of yeast artificial chromosomes containing interleukin genes on human chromosome 5.
To understand better the organization and linkage of the interleukin genes, IL4 and IL5, we prepared long-range restriction maps of five yeast artificial chromosomes (YACs) containing IL5. We determined that IL4 and IL5 are within 100-170 kb, and that the regions surrounding these genes contain several GC-rich areas. Fluorescence in situ chromosomal analysis demonstrated that three of the five YAC clones contain non-contiguous genomic sequences originating from multiple human chromosomes.
Stock
W
W
Department of Medicine, University of Chicago, IL 60637.
Chandrasekharappa
S C
SC
Neuman
W L
WL
Le Beau
M M
MM
Brownstein
B H
BH
Westbrook
C A
CA
D001483:Base Sequence; D002874:Chromosome Mapping; D015825:Chromosomes, Fungal; D002895:Chromosomes, Human, Pair 5; D004277:DNA, Single-Stranded; D016521:Electrophoresis, Gel, Pulsed-Field; D008040:Genetic Linkage; D015894:Genome, Human; D015698:Genomic Library; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D015847:Interleukin-4 / Q000235:genetics*; D015848:Interleukin-5 / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D015183:Restriction Mapping
D004277:DNA, Single-Stranded; D015848:Interleukin-5; D015847:Interleukin-4