Reconstitution of the T cell receptor alpha beta repertoire in recipients of allogeneic BMT.
This report describes the qualitative analysis of T cell receptor (TCR) repertoire regeneration in recipients of BMT. RNA samples from patient and control peripheral blood lymphocytes were prepared and tested for the presence of multiple V alpha and V beta transcripts by the polymerase chain reaction. TCR V gene expression was highly diverse within the first 6 months post-transplantation in recipients receiving either T cell-depleted or T cell-replete marrow, and in HLA mismatched as well as matched donor-recipient pairs. The sequencing of TCR message from BMT recipients also demonstrated J gene diversity and apparently normal junctional diversity at the V-J alpha join. Thus, T cell pools in BMT recipients are largely heterogeneous, not mono- or oligoclonal.
Rencher
S D
SD
St Jude Children's Research Hospital, N. Lauderdale, Memphis 38138.
Hanif
I
I
Heslop
H E
HE
Turner
V E
VE
Brenner
M K
MK
Hurwitz
J L
JL
D000293:Adolescent; D001483:Base Sequence; D016026:Bone Marrow Transplantation / Q000276:immunology*; D002648:Child; D004247:DNA / Q000235:genetics; D015342:DNA Probes; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics*; D014019:Tissue Donors; D014184:Transplantation, Homologous
D015342:DNA Probes; D016693:Receptors, Antigen, T-Cell, alpha-beta; D004247:DNA