Ciprofloxacin-impregnated poly-L-lactic acid drug carrier. New aspects of a resorbable drug delivery system in local antimicrobial treatment of bone infections.
Resorbable poly-L-lactic acid (PLLA) cylinders (3.5 mm diameter, 5 mm in length) carrying 6% of weight ciprofloxacin (Ciprobay, Bayer AG, Leverkusen, FRG) were investigated in vitro to explore their properties as a slow-release antibiotic deposit. Forty bioactive cylinders stored in test tubes were covered with phosphate buffer (pH 7.4 at 37 degrees C) and 40 with fresh human blood plasma and tested under various conditions. For comparison a gentamicin-polymethylmethacrylate (PMMA) chain (Septopal, E. Merck, Darmstadt, FRG) was exposed to similar test conditions. The quantities of ciprofloxacin and gentamicin released were analysed by a microbiological method (bioassay). The concentrations of ciprofloxacin released were analysed by a microbiological method (bioassay). The concentrations of ciprofloxacin released from 40 cylinder were initially very high (up to 180 mg/l) but they decreased rapidly within the first 5 days (4.2-22.5 mg/l). Early release of gentamicin reached up to 227.5 mg/l but dropped to of 22 mg/l on the 14th day. Complete degradation of the PLLA-cylinders was not seen in the observed period of 92 days. The mean loss of mass was 8.4%. The recovery of incorporated ciprofloxacin was 6.5% on average.
Teupe
C
C
Klinik und Poliklinik für Unfall- und Handchirurgie, Westfälischen Wilhelms-Universität Münster, Federal Republic of Germany.
Meffert
R
R
Winckler
S
S
Ritzerfeld
W
W
Törmälä
P
P
Brug
E
E
D001681:Biological Assay; D001847:Bone Diseases / Q000188:drug therapy*; D002939:Ciprofloxacin / Q000008:administration & dosage* / Q000097:blood; D003692:Delayed-Action Preparations; D016503:Drug Delivery Systems; D005839:Gentamicins / Q000008:administration & dosage; D006801:Humans; D007239:Infections / Q000188:drug therapy; D007773:Lactates / Q000008:administration & dosage*; D019344:Lactic Acid; D008768:Methylmethacrylates / Q000008:administration & dosage; D011091:Polyesters; D011108:Polymers / Q000008:administration & dosage*; D013997:Time Factors
D003692:Delayed-Action Preparations; D005839:Gentamicins; D007773:Lactates; D008768:Methylmethacrylates; D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide); D002939:Ciprofloxacin