[The comparative characteristics of the peroxidase conjugates used in Soviet immunoenzyme test systems for detecting HIV antibodies].
Five lots of peroxidase conjugates were studied in enzyme immunoassay systems "Recombinant-HIV" and "Peptoscreen-2" for detection of antibody to HIV. The conjugates differed from each other by the source of generation and methods of preparation. The conjugates were studied in biochemical tests and ELISA. When panels of sera from HIV-infected children and adults were employed, the advantages of using the anti-immunoglobulin conjugate over protein A-peroxidase conjugate were demonstrated, as the former increased the sensitivity of the test systems to HIV antibody detection.
Vorob'eva
M S
MS
Bektimirov
T A
TA
Borsuk
E A
EA
Cheverdiuk
L N
LN
Vaneeva
L I
LI
Shkurina
E A
EA
Kanev
A N
AN
D005069:Evaluation Studies as Topic; D015483:HIV Antibodies / Q000097:blood*; D006801:Humans; D007124:Immunoenzyme Techniques / Q000295:instrumentation*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014586:USSR
D015483:HIV Antibodies
Sravnitel'naia kharakteristika peroksidaznykh kon''iugatov, primeneniaemykh v otechestvennykh immunofermentnykh test-sistemakh dlia vyiavleniia antitel k VICh.