Immunological characterization of a 17-kDa antigen from Cryptosporidium parvum recognized early by mucosal IgA antibodies.
Cryptosporidium parvum antigens were characterized by immunoblot analysis of sera and intestinal secretions of BALB/c mice orally infected with 10(5) oocysts. A major band at 17 kDa under non-reduced conditions and at 18 kDa under reduced conditions was recognized by anti-C. parvum IgA and IgG in serum and intestinal secretions from day 15 post-infection. This recognition persisted throughout the experiment (day 30). Mouse-serum antibodies raised against the 17-kDa purified antigen (P17) showed no cross-reactivity with other C. parvum antigens. Immunofluorescence study revealed that this antigen is located on the sporozoite. It is suggested that this antigen could be a good candidate for studies of mucosal immune response to C. parvum and for vaccination.
Reperant
J M
JM
Institut National de la Recherche Agronomique (I.N.R.A.), Nouzilly, France.
Naciri
M
M
Chardes
T
T
Bout
D T
DT
D000818:Animals; D000913:Antibodies, Protozoan / Q000032:analysis / Q000276:immunology*; D000953:Antigens, Protozoan / Q000032:analysis / Q000276:immunology*; D003457:Cryptosporidiosis / Q000276:immunology*; D016785:Cryptosporidium parvum / Q000276:immunology*; D007071:Immunoglobulin A, Secretory / Q000032:analysis / Q000276:immunology*; D007074:Immunoglobulin G / Q000032:analysis / Q000276:immunology; D007075:Immunoglobulin M / Q000032:analysis / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C
D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D007071:Immunoglobulin A, Secretory; D007074:Immunoglobulin G; D007075:Immunoglobulin M