Preliminary crystallographic studies of the amino terminal half of human lactoferrin in its iron-saturated and iron-free forms.
The amino terminal half of human lactoferrin (LfN) produced from transfected baby hamster kidney cells has been crystallized in its iron-saturated and iron-free forms. The crystals of glycosylated LfN and deglycosylated LfN are monoclinic, space group C2, with cell dimensions a = 133.0 A, b = 58.3 A, c = 58.3 A, alpha = 90.0 degrees, beta = 114.7 degrees, gamma = 90.0 degrees, and one molecule per asymmetric unit. Crystals of apo LfN have also been prepared using deglycosylated protein. These crystals are tetragonal, space group P4(1)2(1)2 (or P4(3)2(1)2), with cell dimensions of a = b = 58.4 A and c = 217.2 A and one molecule per asymmetric unit. Both the iron-saturated and the iron-free crystals are suitable for high resolution X-ray analysis.
Day
C L
CL
Department of Chemistry and Biochemistry, Massey University, Palmerston North, New Zealand.
Norris
G E
GE
Anderson
B F
BF
Tweedie
J W
JW
Baker
E N
EN
D001059:Apoproteins / Q000737:chemistry / Q000378:metabolism; D002478:Cells, Cultured; D003460:Crystallization; D006801:Humans; D007501:Iron / Q000737:chemistry* / Q000378:metabolism; D007781:Lactoferrin / Q000737:chemistry* / Q000378:metabolism; D010446:Peptide Fragments / Q000737:chemistry* / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D014162:Transfection; D014961:X-Ray Diffraction
D001059:Apoproteins; D010446:Peptide Fragments; D011994:Recombinant Proteins; C039223:apolactoferrin; D007501:Iron; D007781:Lactoferrin