Regional distribution of neuropeptide-degrading enzyme activity in the rat brain: effects of subacute exposure to carbon disulfide.
Carbon disulfide, a volatile solvent, is widely used in industry. It has been demonstrated that it causes several neuropsychological symptoms. However, the neurochemical basis of its neurotoxic effect is relatively unknown. In this paper we have measured the effect of subacute i.p. administration on neutral and basic aminopeptidase activities in discrete zones of the rat brain using lysine- and leucine-2-naphthylamides as substrates. Neutral aminopeptidase activity showed a significant decrease in the thalamus and cerebellum with marked (not significant) changes in the hypothalamus, hippocampus, medulla, and occipital cortex. There were no changes in basic aminopeptidase activity. It is suggested that aminopeptidase activity could play a role in carbon disulfide neurotoxic action in the aforementioned regions by generating changes in several neuropeptide levels.
de Gandarias
J M
JM
Department of Physiology, Medical School, University of the Basque Country, Bilbao, Spain.
Echevarría
E
E
Irazusta
J
J
Casis
O
O
Casis
L
L
D015081:2-Naphthylamine / Q000031:analogs & derivatives / Q000378:metabolism; D000626:Aminopeptidases / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000187:drug effects / Q000201:enzymology*; D002246:Carbon Disulfide / Q000633:toxicity*; D007930:Leucine / Q000031:analogs & derivatives / Q000378:metabolism; D008239:Lysine / Q000031:analogs & derivatives / Q000378:metabolism; D008297:Male; D009479:Neuropeptides / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013379:Substrate Specificity; D014018:Tissue Distribution
D009479:Neuropeptides; C078999:lysine-2-naphthylamide; C040135:leucine-beta-naphthylamide; D015081:2-Naphthylamine; D000626:Aminopeptidases; D007930:Leucine; D008239:Lysine; D002246:Carbon Disulfide