title: Molecular basis and allele specific screening of apolipoprotein CIISt. Michael.
abstract: The DNA basis for a circulating nonfunctional variant apoCII, designated apoCIISt. Michael (CII-S) is a shifted reading frame. We developed two amplification primers to screen for CII-S. One contained wild type apoCII sequence and the other contained the sequence for CII-S. Allele-specific polymerase chain reaction (ASPCR) of the apoCII gene from the CII-S proband and her relatives showed unambiguous identification of heterozygous carriers of the CII-S protein. ASPCR may have general applicability in screening for DNA point mutations that do not alter a restriction site.
authors: Department of Medicine, University of Toronto, Ontario, Canada.
authors: R A
authors: RA
authors: Hegele
authors: L
authors: L
authors: Tu
authors: G F
authors: GF
authors: Maguire
authors: P W
authors: PW
authors: Connelly
mesh_terms: D000483:Alleles; D053304:Apolipoprotein C-II; D001056:Apolipoproteins C / Q000235:genetics*; D001483:Base Sequence; D004247:DNA / Q000032:analysis*; D008040:Genetic Linkage; D006801:Humans; D007525:Isoelectric Focusing; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction
chemical_list: D053304:Apolipoprotein C-II; D001056:Apolipoproteins C; C054300:apolipoprotein C-II (St Michael); D004247:DNA