Human urokinase contains GalNAc beta (1-4)[Fuc alpha (1-3)]GlcNAc beta (1-2) as a novel terminal element in N-linked carbohydrate chains.
Structural analysis of enzymically released N-linked carbohydrate chains of human urokinase (urinary-type plasminogen activator) by 1H NMR spectroscopy and FAB-MS demonstrated that the N-linked oligosaccharides on the only N-glycosylation site contain diantennary structures with the novel GalNAc beta (1-4) [Fuc alpha (1-3)]GlcNAc beta (1-2) element in the upper or the lower branch.
Bergwerff
A A
AA
Bijvoet Center, Department of Bio-Organic Chemistry, Utrecht University, The Netherlands.
Thomas-Oates
J E
JE
van Oostrum
J
J
Kamerling
J P
JP
Vliegenthart
J F
JF
D002240:Carbohydrate Sequence; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D016339:Spectrometry, Mass, Fast Atom Bombardment; D014568:Urokinase-Type Plasminogen Activator / Q000737:chemistry*
D014568:Urokinase-Type Plasminogen Activator