Ectopic transcription of the parathyroid hormone gene in lymphocytes, lymphoblastoid cells and tumour tissue.
Using reverse transcription polymerase chain reaction, steady-state levels of parathyroid hormone (PTH) mRNA were investigated in a number of human, bovine and rat tissues. Transcripts were consistently detected in parathyroid glands as well as in lymphocytes, lymphoblastoid cells and several tumours. Levels of transcription were not measurably increased in lymphoblastoid cells and tumour tissues compared with unstimulated peripheral lymphocytes. The level of 'ectopic' transcription of the PTH gene in lymphoblastoid cells appeared to be resistant to the administration of both vitamin D and phorbol esters.
Handt
O
O
Institut für Humangenetik, Freien Universität Berlin, Germany.
Reis
A
A
Schmidtke
J
J
D000818:Animals; D001943:Breast Neoplasms / Q000235:genetics*; D002417:Cattle; D006801:Humans; D007945:Leukemia, Lymphoid / Q000235:genetics*; D008214:Lymphocytes / Q000502:physiology*; D010280:Parathyroid Glands / Q000502:physiology; D010281:Parathyroid Hormone / Q000235:genetics*; D010703:Phorbol Esters / Q000494:pharmacology; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D014158:Transcription, Genetic / Q000502:physiology*; D014807:Vitamin D / Q000494:pharmacology
D010281:Parathyroid Hormone; D010703:Phorbol Esters; D012333:RNA, Messenger; D014807:Vitamin D