Sex determination in sheep and goats using bovine Y-chromosome specific primers via polymerase chain reaction: potential for embryo sexing.
A simple and novel method, using polymerase chain reaction (PCR) has been standardized for accurate sex determination in sheep and goats. The assay utilizes a pair of bovine Y-chromosome specific primers and the genomic DNA isolated from blood samples of adult male and female sheep and goats. The primers recognize and amplify the Y-chromosome specific sequences in male goats and sheep. The assay is accurate, reliable and rapid.
Rao
K B
KB
Embryo Biotechnology Laboratory, National Institute of Immunology, New Delhi, India.
Totey
S M
SM
D000822:Animal Husbandry; D000818:Animals; D001483:Base Sequence; D001755:Blastocyst; D002417:Cattle; D004247:DNA / Q000235:genetics; D015342:DNA Probes; D005260:Female; D006041:Goats; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D011247:Pregnancy; D012732:Sex Determination Analysis / Q000379:methods*; D012756:Sheep; D014998:Y Chromosome
D015342:DNA Probes; D004247:DNA