title: Immunological properties of a radioiodinated monoclonal antibody and its F(ab')2mu fragments directed against the polymorphic epithelial mucin of human breast cancer.
abstract: The purification of the IgM monoclonal antibody 436 against a breast tumor antigen from mouse ascitic fluid is reported. The purified immunoglobulin was radioiodinated and the resulting product assessed for its binding capacity and binding specificity. Purified IgM-436 served for F(ab')2 mu preparation which was tested for its antigen binding capacity. Radioiodinated IgM-436 and its F(ab')2 mu retained their immunological activity which was never lower than those of the corresponding cold products.
authors: Section of Nuclear Medicine, University of Roma, La Sapienza, Italy.
authors: M
authors: M
authors: Liberatore
authors: M
authors: M
authors: Nuti
authors: G
authors: G
authors: Cascialli
authors: V
authors: V
authors: Turchi
authors: M
authors: M
authors: Clemente
authors: G
authors: G
authors: Rossetto
authors: A
authors: A
authors: Centi Colella
authors: A
authors: A
authors: Pala
mesh_terms: D000911:Antibodies, Monoclonal / Q000302:isolation & purification; D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor / Q000276:immunology*; D001943:Breast Neoplasms / Q000276:immunology*; D004848:Epithelium / Q000276:immunology; D005260:Female; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000302:isolation & purification; D007075:Immunoglobulin M / Q000302:isolation & purification; D007457:Iodine Radioisotopes; D009077:Mucins / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D007140:Immunoglobulin Fab Fragments; D007075:Immunoglobulin M; D007457:Iodine Radioisotopes; D009077:Mucins