Phytanic acid alpha-oxidation in human cultured skin fibroblasts.
We studied the oxidation of [1-14C]phytanic acid, 3-methyl substituted fatty acid, to pristanic acid and 14CO2 in human skin fibroblasts. The specific activity for alpha-oxidation of phytanic acid in peroxisomes was 29- and 124-fold higher than mitochondria and endoplasmic reticulum. This finding demonstrates for the first time the presence of fatty acid alpha-oxidation enzyme system in peroxisomes.
Singh
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Department of Pediatrics, Medical University of South Carolina, Charleston 29425.
Lazo
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Kalipada
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Singh
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AK
D002458:Cell Fractionation; D002478:Cells, Cultured; D003066:Coenzyme A Ligases / Q000378:metabolism; D004852:Epoxy Compounds / Q000494:pharmacology; D005227:Fatty Acids / Q000378:metabolism; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D010084:Oxidation-Reduction; D010831:Phytanic Acid / Q000494:pharmacology*; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; D012867:Skin / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D013347:Subcellular Fractions / Q000378:metabolism; D000098859:Long-Chain-Fatty-Acid-CoA Ligase
D004852:Epoxy Compounds; D005227:Fatty Acids; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; D010831:Phytanic Acid; C002844:pristanic acid; C033891:ethyl 2-(5-(4-chlorophenyl)pentyl)oxiran-2-carboxylate; D003066:Coenzyme A Ligases; C044125:lignoceroyl-CoA ligase; C090799:FAA2 protein, S cerevisiae; D000098859:Long-Chain-Fatty-Acid-CoA Ligase