The TraM protein of the conjugative plasmid F binds to the origin of transfer of the F and ColE1 plasmids.
The gene encoding the TraM protein of the conjugative plasmid F was cloned, overexpressed and the gene product was purified. The TraM protein was found in the cytoplasm of cells carrying the F plasmid with a smaller amount in the inner membrane. DNase I footprinting experiments showed that the purified protein protects three regions in the F oriT locus with different affinity for the upper and lower strands of DNA. A 15-nucleotide motif was identified within the protected regions that represented the DNA-binding site. The TraM protein was also found to bind to a sequence in the oriT region of the non-conjugative plasmid ColE1 that resembles the three binding sites in the F oriT region.
Di Laurenzio
L
L
Department of Biochemistry, University of Alberta, Edmonton, Canada.
Frost
L S
LS
Paranchych
W
W
D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D003086:Bacteriocin Plasmids / Q000235:genetics*; D001483:Base Sequence; D002874:Chromosome Mapping; D003227:Conjugation, Genetic; D016384:Consensus Sequence; D004261:DNA Replication; D004926:Escherichia coli / Q000235:genetics*; D005144:F Factor / Q000235:genetics*; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism
D001426:Bacterial Proteins; D009838:Oligodeoxyribonucleotides; D011994:Recombinant Proteins; C074281:TraM protein, bacterial