Distribution and properties of endo-oligopeptidases A and B in the human neuroendocrine system.
The thiol activated endo-oligopeptidases A and B were studied in the soluble fraction of human hypothalamus and various endocrine glands. For the identification, characterization and purification of the enzymes, Z-Gly-Pro-NH-Np and bradykinin were used as substrates. Endo-oligopeptidase B showed a molecular mass ranging from 55.5 to 65.5 kDa and isoelectric point from 4.7 to 4.95. Its activity in tissues was highest in the testis, with intermediate levels in the thyroid, neurohypophysis, adenohypophysis and hypothalamus and the lowest activity in the pineal gland. Endo-oligopeptidase A, 467-fold purified by immunoaffinity chromatography, exhibited a molecular mass of 65.5 kDa in the adenohypophysis but 58.5 kDa in other tissues. The isoelectric point ranged from 5.22 to 5.50. High endo-oligopeptidase A activity was observed in the adenohypophysis, testis and hypothalamus with lesser activity in the neurohypophysis and thyroid and the lowest in the pineal gland. Endo-oligopeptidase A cleaved the bonds Phe-Ser of bradykinin, Met-Arg of BAM-12P and Arg-Arg of neurotensin as described for rabbit brain and heart and bovine brain enzymes. This work shows that endo-oligopeptidase A also hydrolysed the bonds Tyr-Gly of LH-releasing hormone, Pro-Phe of angiotensin I and Tyr-Ile of angiotensin II.
Medeiros
M S
MS
Department of Clinical Medicine, Faculty of Medicine of Ribeirão Preto, University of São Paulo, Brazil.
Iazigi
N
N
Camargo
A C
AC
Oliveira
E B
EB
D000293:Adolescent; D000328:Adult; D003546:Cysteine Endopeptidases / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D007031:Hypothalamus / Q000201:enzymology; D008297:Male; D008666:Metalloendopeptidases / Q000737:chemistry / Q000378:metabolism*; D009490:Neurosecretory Systems / Q000201:enzymology*; D010870:Pineal Gland / Q000201:enzymology; D010902:Pituitary Gland / Q000201:enzymology; D013737:Testis / Q000201:enzymology; D013961:Thyroid Gland / Q000201:enzymology
D003546:Cysteine Endopeptidases; C028307:endopeptidase B; D008666:Metalloendopeptidases; C022510:thimet oligopeptidase