Characterization of nucleotide sequences that interact with a nuclear protein fraction in rRNA gene of Vicia faba.
A certain nucleotide sequence in the promoter region of Vicia faba rRNA genes that specifically binds to a nuclear protein fraction has been identified by using a gel retardation assay and DNase I footprinting technique. The binding site of this protein fraction is located about 60 bp upstream from the initiation site of the pre-rRNA transcript. This location does not correspond with previously reported results on maize rRNA genes. However, both of the binding sites share a bi-partite consensus sequence, TAT-G(N)xCAGG. Methylation interference experiments show that two G residues in TATG and the complementary strand of CAGG are important for specific DNA-protein interaction. Furthermore, competition analyses using point-mutated synthetic DNAs show that two G residues in CAGG are essential for this interaction. Similar sequences are found in promoter regions of other plant and animal rRNA genes. We suggest that these sequences may be a cis-control element commonly involved in rRNA transcription.
Nakajima
T
T
Department of Botany, Faculty of Science, Hokkaido University, Sapporo, Japan.
Suzůki
A
A
Tanifuji
S
S
Kato
A
A
D001483:Base Sequence; D001665:Binding Sites; D004275:DNA, Ribosomal / Q000235:genetics / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000302:isolation & purification / Q000378:metabolism*; D003850:Deoxyribonuclease I; D008745:Methylation; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000302:isolation & purification / Q000378:metabolism*; D010944:Plants / Q000235:genetics / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D012335:RNA, Ribosomal / Q000235:genetics*; D015183:Restriction Mapping; D017384:Sequence Deletion; D013698:Templates, Genetic
D004275:DNA, Ribosomal; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D012335:RNA, Ribosomal; D003850:Deoxyribonuclease I