title: Collection of eggs and hatching and culturing second-stage larvae of Toxocara vitulorum in vitro.
abstract: Eggs of Toxocara vitulorum were harvested from the feces of infected buffalo calves and embryonated in vitro. Optimum conditions for hatch and culture of the second-stage larvae were determined. Maximum hatch of larvae occurred from eggs that were decoated by treatment with saturated Ca(OCl)2 for 16-24 min followed by treatment with CO2 and incubation at 37 C. Larvae could be cultured in RPMI-1640 medium for up to 3 mo but survived for only 3 wk in Eagle's minimum essential medium.
authors: Faculty of Veterinary Medicine and Animal Science, University of Peradeniya, Sri Lanka.
authors: R P
authors: RP
authors: Rajapakse
authors: V W
authors: VW
authors: Vasanthathilake
authors: S
authors: S
authors: Lloyd
authors: S T
authors: ST
authors: Fernando
mesh_terms: D000818:Animals; D002020:Buffaloes / Q000469:parasitology*; D005243:Feces / Q000469:parasitology; D007814:Larva / Q000254:growth & development; D010063:Ovum; D014119:Toxocara / Q000254:growth & development* / Q000302:isolation & purification; D014120:Toxocariasis / Q000469:parasitology*