Regulation of glucose transport in isolated adipocytes by levamisole.
When isolated rat adipocytes were incubated with increasing concentrations of levamisole (0.5-5 mM), basal glucose oxidation decreased by almost 50% and insulin-stimulated glucose oxidation decreased by 90%. The decrease in glucose oxidation correlated with an inhibition of glucose transport, since levamisole at 5.0 mM decreased basal 3-O-methylglucose transport by 60% and insulin-stimulated transport by 80%. Diamide-stimulated glucose transport was also inhibited approximately 80% by 5.0 mM levamisole. Levamisole at concentrations up to 5.0 mM had no effect on phosphofructokinase activity. The present results suggest that levamisole inhibits glucose utilization by inhibiting glucose transport in a concentration-dependent manner.
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Department of Biochemistry and Molecular Biology, Howard University College of Medicine, Washington, D.C. 20059.
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D019325:3-O-Methylglucose; D000273:Adipose Tissue / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D003958:Diamide / Q000494:pharmacology; D005947:Glucose / Q000378:metabolism*; D066298:In Vitro Techniques; D007328:Insulin / Q000494:pharmacology; D007978:Levamisole / Q000494:pharmacology*; D008297:Male; D008757:Methylglucosides / Q000378:metabolism; D010084:Oxidation-Reduction; D010732:Phosphofructokinase-1 / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
D007328:Insulin; D008757:Methylglucosides; D003958:Diamide; D019325:3-O-Methylglucose; D007978:Levamisole; D010732:Phosphofructokinase-1; D005947:Glucose