5'-Nucleotidase immunoreactivity of perineuronal microglia responding to rat facial nerve axotomy.
The ecto-enzyme 5'-nucleotidase was localized immunocytochemically in the axotomized rat facial nucleus. As revealed by the monoclonal antibody 5N4-2,5'-nucleotidase immunoreactivity markedly increased on perineuronal microglia during the first week following axotomy, and gradually disappeared from these cells by the end of the third post-operative week. Interestingly, parenchymal microglia were not or only weakly stained. These findings indicate that 5'-nucleotidase 5N4-2-immunoreactivity may serve as a marker for perineuronal microglia, a population of satellite glial cells that appear to be actively engaged in lesion-induced synaptic changes during regeneration.
Schoen
S W
SW
Department of Neuromorphology, Max-Planck-Institute of Psychiatry, Martinsried, Germany.
Graeber
M B
MB
Kreutzberg
G W
GW
D015720:5'-Nucleotidase / Q000378:metabolism*; D000818:Animals; D001369:Axons / Q000502:physiology*; D003714:Denervation; D005154:Facial Nerve / Q000378:metabolism / Q000502:physiology*; D007150:Immunohistochemistry; D009457:Neuroglia / Q000201:enzymology*; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D014018:Tissue Distribution
D015720:5'-Nucleotidase