Interferon regulatory factor-1 (IRF-1) activates the synthetic IRF-1-responsive sequence (GAAAGT)4 in Saccharomyces cerevisiae.
In appropriate mammalian cells, interferon regulatory factor-1 (IRF-1) can activate the virus-responsive element of the IFN-beta promoter (VRE beta") or the synthetic oligonucleotide (GAAAGT)4. The latter contains two copies of the functional equivalent of PRDI, one of the regulatory domains of VRE beta". We prepared yeast strains containing an IRF-1 expression plasmid under the control of the galactose-inducible Gal1 promoter and a reporter plasmid with either (GAAAGT)4, VRE beta", or other test sequences placed upstream of a minimal promoter linked to the beta-galactosidase coding sequence. Upon induction of IRF-1 expression, the (GAAAGT)4-containing promoter was activated, but VRE beta" and all other sequences tested were inactive. Our results showed that IRF-1 belongs to a class of higher eukaryotic transcription factors that can interact with the yeast transcriptional machinery. Our findings also raised the question why the duplicate PRDI-like sequences in (GAAAGT)4 can be activated by IRF-1 synthesized in yeast, but not VRE beta", which also contains at least two PRDI-like sequences.
Sailer
A
A
Institut für Molekularbiologie I, Universität Zürich, Switzerland.
Nagata
K
K
Näf
D
D
Aebi
M
M
Weissmann
C
C
D001483:Base Sequence; D004268:DNA-Binding Proteins / Q000502:physiology*; D015966:Gene Expression Regulation, Fungal; D005822:Genetic Vectors; D050856:Interferon Regulatory Factor-1; D016899:Interferon-beta / Q000235:genetics*; D008969:Molecular Sequence Data; D010750:Phosphoproteins / Q000502:physiology*; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics; D012045:Regulatory Sequences, Nucleic Acid; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D015533:Transcriptional Activation; D001616:beta-Galactosidase / Q000096:biosynthesis
D004268:DNA-Binding Proteins; D050856:Interferon Regulatory Factor-1; D010750:Phosphoproteins; D011993:Recombinant Fusion Proteins; D016899:Interferon-beta; D001616:beta-Galactosidase