The amino acid sequence of a type I copper protein with an unusual serine- and hydroxyproline-rich C-terminal domain isolated from cucumber peelings.
We have determined the amino acid sequence of a small copper protein isolated from cucumber peelings. This cupredoxin contains 137 amino acids including a pyroglutamate as the first residue. The N-terminal 110 amino acid-long domain shows 30-37% identity to 2 other cupredoxins, stellacyanin and cucumber basic blue protein. A unique feature of this protein is a 27 amino acid-long C-terminal domain rich in 4-hydroxyproline and serine and resembling certain plant cell wall proteins. The prolines in this domain are hydroxylated to a different extent depending on the surrounding sequence.
Mann
K
K
Max-Planck-Institut für Biochemie, Martinsried, Germany.
Schäfer
W
W
Thoenes
U
U
Messerschmidt
A
A
Mehrabian
Z
Z
Nalbandyan
R
R
D000595:Amino Acid Sequence; D001400:Azurin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid; D003300:Copper / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D006909:Hydroxyproline / Q000737:chemistry*; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000737:chemistry* / Q000302:isolation & purification; D010944:Plants; D017386:Sequence Homology, Amino Acid; D012694:Serine / Q000737:chemistry*
D010940:Plant Proteins; C050107:cupredoxin; D001400:Azurin; D012694:Serine; D003300:Copper; D006909:Hydroxyproline