A colorimetric microassay for the detection of agents that interact with DNA.
A simple microtiter assay for the detection of compounds that bind DNA is described. Agents that displace methyl green from DNA are detected spectrophotometrically by a decrease in absorbance at 630 nm. The feasibility of using the assay for detecting DNA-active compounds in fermentation extracts was assessed, and the activities of reference compounds in the methyl green assay and an ethidium bromide displacement method were compared.
Burres
N S
NS
Bioactive Microbial Metabolites, Abbott Laboratories, Abbott Park, Illinois 60064.
Frigo
A
A
Rasmussen
R R
RR
McAlpine
J B
JB
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology; D003124:Colorimetry; D004247:DNA / Q000032:analysis / Q000187:drug effects*; D004996:Ethidium; D005285:Fermentation; D008739:Methyl Green; D013050:Spectrometry, Fluorescence
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D000903:Antibiotics, Antineoplastic; D008739:Methyl Green; C036275:chrysomycin B; D004247:DNA; D004996:Ethidium