Luminometric measurement of population activity of genetically modified Pseudomonas fluorescens in the soil.
Genetically modified cells of Pseudomonas fluorescens, chromosomally marked with genes for bioluminescence, were inoculated into sterile soil microcosms. During incubation for 90 days, viable cell concentration did not change significantly but light output, measured by luminometry, decreased, indicating reduced metabolic activity due to lack of substrates. Amendment with nutrients resulted in parallel increases in both luminescence and dehydrogenase activity. Luminometry therefore enables rapid monitoring of the activity of populations of luminescence-marked microbial inocula in the soil, with greater sensitivity and selectivity than traditional techniques.
Meikle
A
A
Department of Molecular and Cell Biology, University of Aberdeen, Marischal College, UK.
Killham
K
K
Prosser
J I
JI
Glover
L A
LA
D001431:Bacteriological Techniques; D015169:Colony Count, Microbial; D005069:Evaluation Studies as Topic; D005819:Genetic Markers; D008156:Luciferases / Q000235:genetics; D008163:Luminescent Measurements; D011551:Pseudomonas fluorescens / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D012988:Soil Microbiology
D005819:Genetic Markers; D008156:Luciferases