title: Direct complementary DNA cloning and screening of mutants using polymerase chain reaction.
authors: Department of Immunology, Genetic Therapy Inc., Gaithersburg, Maryland 20878.
authors: Y L
authors: YL
authors: Chiang
mesh_terms: D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular / Q000379:methods*; D015936:Complement C5a / Q000276:immunology; D004247:DNA / Q000235:genetics*; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007127:Immunoglobulin Constant Regions / Q000235:genetics; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D007147:Immunoglobulin Light Chains / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D016376:Oligonucleotides, Antisense; D016133:Polymerase Chain Reaction / Q000379:methods*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis
chemical_list: D007127:Immunoglobulin Constant Regions; D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains; D009838:Oligodeoxyribonucleotides; D016376:Oligonucleotides, Antisense; D011993:Recombinant Fusion Proteins; D015936:Complement C5a; D004247:DNA