[Effects of the fraction (1-10 kDa) of the brain of a Yakut horse on kinetic parameters of Ca2+ transport system in sarcolemma vesicles of cardiomyocytes].
The effect of the fraction (1-10 kDa) obtained from the brain of cold-adapted animal (Yakut horse) on Ca2+ transport in sarcolemma vesicles of cardiomyocytes was investigated. It was shown that during insertion of Yakut horse brain fraction into incubation medium at the concentration from 10(-9) M to 3.10(-5) M at Ca2+ transport substrate concentration from 0.1 mM to 1.0 mM, the rate of Ca2+ passive penetration into vesicles slightly increased and at Ca2+ transport substrate concentration 3 mM, which is physiologic, a decrease of rate values was established for all concentrations of the fraction (1-10 kDa) of Yakut horse. While studying the kinetics of an active Ca2+ transport for all investigated concentrations of the fraction (1-10 kDa) of Yakut horse brain from 10(-9) M to 3.10(-5) M at Ca2+ concentration in incubation medium from 10(-7) to 3.10(-6) M, calcium accumulation rates by vesicles exceeded control values. So we can suppose that application of brain fraction (1-10 kDa) of genotypically cold-adapted animal, results in a decrease of intracellular Ca2+ concentration.
Gulevskiĭ
A K
AK
Grishchenko
V I
VI
Tereshchenko
O S
OS
Zagnoĭko
V I
VI
Akhremenko
A K
AK
Kucherenko
S N
SN
D000222:Adaptation, Physiological; D000818:Animals; D001921:Brain / Q000502:physiology*; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D003081:Cold Climate; D006736:Horses / Q000502:physiology*; D009200:Myocardial Contraction; D009206:Myocardium / Q000166:cytology / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000502:physiology*; D012508:Sarcolemma / Q000378:metabolism*; D012800:Siberia
D009419:Nerve Tissue Proteins; D002118:Calcium
Vliianie fraktsii (1-10 kDa) mozga iakutskoĭ loshadi na kineticheskie parametry Ca2+-transportiruiushchikh sistem v vezikulakh sarkolemmy kardiomiotsitov.