Quantitative analysis of MEL-14 expression on various lymphocyte subpopulations.
Amounts of gp90MEL-14 molecules in various lymphocyte subpopulations were analyzed. The expression of gp90MEL-14 was rapidly up-regulated in the course of usual procedures for preparing single cell suspensions. On the other hand, the gp90MEL-14 expression was down-modulated after short term culture in the presence of PMA. Addition of NaN3 in the medium for preparing cell suspension was shown to stabilize the expression profile of the gp90MEL-14. By this simple improvement, we could successfully evaluate the precise amounts of gp90MEL-14 expressed on various lymphoid cells which appeared to reflect the expression pattern of gp90MEL-14 in vivo. The proportion of MEL-14+ cells and amounts of gp90MEL-14 on the cells were clearly associated with the anatomical and functional distance from endothelial cells of HEV in peripheral lymph nodes. The present findings, thus, support the role of gp90MEL-14 molecules as homing receptors for HEV that was originally proposed by Weissman and his colleagues.
Ohgama
J
J
Section of Pathology, Hokkaido University, Sapporo, Japan.
Onoé
K
K
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D001386:Azides / Q000494:pharmacology; D015704:CD4 Antigens / Q000096:biosynthesis; D016827:CD8 Antigens / Q000096:biosynthesis; D005260:Female; D005434:Flow Cytometry; D005786:Gene Expression Regulation / Q000187:drug effects; D008198:Lymph Nodes / Q000378:metabolism; D016131:Lymphocyte Subsets / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D010581:Peyer's Patches / Q000378:metabolism; D016201:Receptors, Lymphocyte Homing; D019810:Sodium Azide; D013154:Spleen / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D013950:Thymus Gland / Q000378:metabolism
D000911:Antibodies, Monoclonal; D001386:Azides; D015704:CD4 Antigens; D016827:CD8 Antigens; D016201:Receptors, Lymphocyte Homing; D019810:Sodium Azide; D013755:Tetradecanoylphorbol Acetate