Kinetics of Lys-bradykinin release by porcine pancreatic kallikrein from rabbit low molecular weight kininogen.
The initial rates of Lys-bradykinin release by porcine pancreatic kallikrein from rabbit low molecular weight kininogen are found to follow the Michaelis-Menten kinetics with kc = 0.62 sec-1 and Km = 1.93 microM at substrate concentrations 0.3-1.3 microM, but at higher ones the Michaelis dependence is broken. Inhibition of the reaction by its product(s) with Kp < Km is revealed with integral analysis methods in a range of 4.5-270 microM kininogen.
Makevnina
L G
LG
Institute of Biological and Medical Chemistry, Moscow.
Levina
G O
GO
Yatzimirsky
A K
AK
D000818:Animals; D006868:Hydrolysis; D066298:In Vitro Techniques; D007609:Kallidin / Q000378:metabolism*; D007610:Kallikreins / Q000378:metabolism*; D007700:Kinetics; D007704:Kininogens / Q000378:metabolism*; D010179:Pancreas / Q000201:enzymology; D011817:Rabbits; D013379:Substrate Specificity; D013552:Swine
D007704:Kininogens; D007609:Kallidin; D007610:Kallikreins