title: The application of AgNOR technique on glycol-methacrylate embedded human normal and pathological tissues.
abstract: The Authors have applied the AgNOR technique to a series of normal and pathological human tissues fixed in neutral buffered formalin and methacarnoy and embedded in glycol-methacrylate. The results show the compatibility of the AgNOR technique with this embedding medium as well as the possibility to obtain an adequate visualization of the specific silver precipitates (AgNORs) on a unique focal plane without appreciable nuclear overlap, making thus the valuation of the AgNORs less subjective.
authors: Dipartimento di Patologia Umana, Policlinico Universitario, Messina.
authors: G
authors: G
authors: Tuccari
authors: M
authors: M
authors: Muscarà
authors: G
authors: G
authors: Giuffrè
authors: C
authors: C
authors: Crisafulli
authors: G
authors: G
authors: Barresi
mesh_terms: D004064:Digestive System / Q000033:anatomy & histology; D005404:Fixatives; D006801:Humans; D008689:Methacrylates; D009369:Neoplasms / Q000473:pathology*; D009697:Nucleolus Organizer Region / Q000648:ultrastructure*; D016613:Plastic Embedding; D016622:Silver Staining; D013048:Specimen Handling; D013961:Thyroid Gland / Q000033:anatomy & histology
chemical_list: D005404:Fixatives; D008689:Methacrylates; C005044:hydroxyethyl methacrylate