Developmental regulation of phosphoprotein gene expression in the caudate-putamen of rat: an in situ hybridization study.
The regional and cellular ontogeny of the mRNA encoding the dopamine- and cAMP-regulated phosphoprotein, DARPP-32, has been studied in rat striatum by quantitative in situ hybridization histochemistry. The mRNA for DARPP-32 exhibited a characteristic developmental profile. The hybridization signal was first visible on the day of birth, at which time DARPP-32 mRNA was concentrated in patches in the caudate-putamen. By the end of the first postnatal week, the majority of neurons in the caudate-putamen expressed the DARPP-32 message. Levels of mRNA per cell increased markedly during the second postnatal week, and peaked around the beginning of the third week. The adult level of DARPP-32 mRNA was lower than that observed at the apex of mRNA expression, on a per cell basis, while the proportion of neurons expressing detectable levels of message remained relatively constant. In the nucleus accumbens and olfactory tubercle, DARPP-32 mRNA development lagged somewhat behind that observed in the caudate-putamen, but was similar in other respects. A non-quantitative study employing an oligonucleotide probe complementary to the mRNA encoding another cAMP-regulated phosphoprotein, ARPP-21, revealed a similar developmental sequence to DARPP-32. The present results suggest that for DARPP-32 mRNA, genetic and, possibly, environmental factors play a role in determining the developmental patterns observed.
Gustafson
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Laboratory of Molecular and Cellular Neuroscience, Rockefeller University, New York, NY 10021.
Ehrlich
M E
ME
Trivedi
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P
Greengard
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P
D000375:Aging / Q000502:physiology*; D000818:Animals; D001345:Autoradiography; D001483:Base Sequence; D001921:Brain / Q000378:metabolism; D002421:Caudate Nucleus / Q000196:embryology / Q000254:growth & development / Q000378:metabolism*; D004247:DNA / Q000235:genetics; D051937:Dopamine and cAMP-Regulated Phosphoprotein 32; D005314:Embryonic and Fetal Development; D005786:Gene Expression Regulation; D005865:Gestational Age; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000096:biosynthesis / Q000235:genetics*; D009838:Oligodeoxyribonucleotides; D009928:Organ Specificity; D010750:Phosphoproteins / Q000096:biosynthesis / Q000235:genetics*; D011699:Putamen / Q000196:embryology / Q000254:growth & development / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013462:Sulfur Radioisotopes
D051937:Dopamine and cAMP-Regulated Phosphoprotein 32; D009419:Nerve Tissue Proteins; D009838:Oligodeoxyribonucleotides; D010750:Phosphoproteins; D012333:RNA, Messenger; D013462:Sulfur Radioisotopes; D004247:DNA