RF-plasma-modified polystyrene surfaces for studying complement activation.
Five different plasma modified surfaces were made for studying different aspects of biocompatibility. These surfaces were: 1,2-diaminocyclohexane (DACH), acrylic acid (AA), Hydroxyethylmethacrylate (HEMA), methane and hexamethylene-disiloxane (HMDSO). In addition a polyethylene-glycol (PEG) was made by grafting aldehyde functional PEG to the DACH surface. PEG and HMDSO which are the most hydrophilic and the most hydrophobic surface shows the lowest amount of adsorbed protein of the three proteins studied here (albumin, IgG and C3). Methane, HMDSO and HEMA was found to activate via the classical (complement activation) pathway while the others activated via the alternative pathway.
Gölander
C G
CG
Institute for Surface Chemistry, Stockholm, Sweden.
Lassen
B
B
Nilsson-Ekdahl
K
K
Nilsson
U R
UR
D000327:Adsorption; D003167:Complement Activation / Q000187:drug effects*; D003176:Complement C3 / Q000187:drug effects*; D015394:Molecular Structure; D011092:Polyethylene Glycols / Q000737:chemistry*; D011108:Polymers; D011137:Polystyrenes / Q000737:chemistry*; D011485:Protein Binding / Q000502:physiology; D013499:Surface Properties
D003176:Complement C3; D011108:Polymers; D011137:Polystyrenes; D011092:Polyethylene Glycols