Human immunoglobulin genes of the kappa type. The long-range map of an orphon V kappa gene region.
As was previously shown by Zimmer et al. (EMBO J. 9, 1535-1542, 1990 and Biol. Chem. Hoppe-Seyler 371, 939-951, 1990), the so-called W regions comprising 11 V kappa pseudogenes are located on the long arm of chromosome 2, very closely to the centromere. They are probably derived by a pericentric inversion and amplification events from gene regions of the kappa locus, which is located on the short arm of chromosome 2 also very closely to the centromere. The restriction map of the W regions was now extended from the previous 1.2 Mb to 4.3 Mb and, at the same time, revised with respect to certain features. This was made possible by a new hybridization probe specific for the Wc region and by the improved resolution and extended range of pulsed field gel electrophoresis. On the basis of the long-range maps of the W regions and the kappa locus the V kappa genes of the kappa locus have to be at least 2.5 Mb apart. This distance can be taken also as a minimal estimate for the size of the centromere DNA of chromosome 2; it is quite possible that the size is much larger.
Weichhold
G M
GM
Institut für Physiologische Chemie, Universität München, Germany.
Lautner-Rieske
A
A
Zachau
H G
HG
D002874:Chromosome Mapping; D002889:Chromosomes, Human, Pair 2 / Q000737:chemistry; D016521:Electrophoresis, Gel, Pulsed-Field; D005803:Genes, Immunoglobulin; D006801:Humans; D007145:Immunoglobulin kappa-Chains / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D011544:Pseudogenes; D015183:Restriction Mapping
D007145:Immunoglobulin kappa-Chains