title: Study on the mechanism of an experimental immunological intrahepatic cholestasis model.
abstract: Tuberculin-sensitized guinea pigs were intravenously injected with heat-killed Propionibacterium acnes followed by an intravenous injection of purified protein derivatives 7 day later, resulting in the induction of intrahepatic cholestasis. Using this experimental model, the following results were obtained: (1) Both uptake and release of bile acid were inhibited in the hepatocytes prepared from the cholestasis guinea pigs. (2) The results of the erythritol clearance method indicated that the decrease in bile flow observed in the cholestasis guinea pigs was mostly attributable to the reduced bile excretion from the canaliculi. (3) The decrease in the formation of bile acid independent bile flow was the cause of the decrease in bile flow observed in the cholestasis guinea pigs. (4) There was no change in the permeability of the interhepatocellular tight junction in the cholestasis guinea pigs.
authors: Third Department of Internal Medicine, Osaka City University Medical School, Japan.
authors: T
authors: T
authors: Shin
authors: Y
authors: Y
authors: Mizoguchi
authors: K
authors: K
authors: Kioka
authors: K
authors: K
authors: Kobayashi
authors: S
authors: S
authors: Morisawa
mesh_terms: D000818:Animals; D001646:Bile / Q000378:metabolism; D001647:Bile Acids and Salts / Q000378:metabolism; D002780:Cholestasis, Intrahepatic / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D006168:Guinea Pigs; D008099:Liver / Q000378:metabolism / Q000473:pathology; D008222:Lymphokines / Q000378:metabolism; D008297:Male; D011425:Propionibacterium acnes / Q000276:immunology; D014373:Tuberculin / Q000276:immunology; D015164:Vaccines, Inactivated / Q000276:immunology
chemical_list: D001647:Bile Acids and Salts; D008222:Lymphokines; D014373:Tuberculin; D015164:Vaccines, Inactivated