[Changes in the frequency of cells with micronuclei in cell populations of rat transplantable rhabdomyosarcoma RA-2 as a result of selection for an increased and a decreased frequency of such cells].
Artificial selection for increasing and decreasing frequencies of cells with micronuclei (FCM) was conducted in populations of cell clones of the rat transplantable RA-2 rabdomyosarcoma. The selection was effective in both the opposite directions. The average FCM increased from 3.3 +/- 0.2 to 6.9 +/- 0.6% and decreased from 3.3 +/- 0.2 to 1.6 +/- 0.2%. Heritability coefficient, h2, varied from 0.2 to 0.4. In the cell population with increased FCM, 37% of anaphases and early telophases had lagged chromosomes and fragments. In contrast, in the cell population with decreased FCM, lagging were observed in 11% of cases. The DNA-content in G1 cell varied greatly in cell population with increased FCM (P < 0.01). The selection for increased FCM has led to a significant decrease in metastatic capacities of cells.
Kravtsov
V Iu
VIu
Rozanov
Iu M
IuM
Kaminskaia
E V
EV
Iakovlev
A F
AF
Vakhtin
Iu B
IuB
D000818:Animals; D004273:DNA, Neoplasm / Q000032:analysis; D005434:Flow Cytometry; D007621:Karyotyping; D008168:Lung / Q000737:chemistry / Q000648:ultrastructure; D008175:Lung Neoplasms / Q000737:chemistry / Q000556:secondary / Q000648:ultrastructure*; D048629:Micronuclei, Chromosome-Defective / Q000737:chemistry / Q000648:ultrastructure*; D009368:Neoplasm Transplantation; D051381:Rats; D012208:Rhabdomyosarcoma / Q000737:chemistry / Q000556:secondary / Q000648:ultrastructure*; D012641:Selection, Genetic; D013997:Time Factors; D014407:Tumor Cells, Cultured
D004273:DNA, Neoplasm
Izmenenie chastoty kletok s mikroiadrami v kletochnykh populiatsiiakh perevivnoĭ rabdomiosarkomy RA-2 krys v resul'tate otbora na povyshennuiu i ponizhennuiu chastotu takikh kletok.