Calcium and carbonate supply in the shell gland of hens laying eggs with strong and weak shells and during and after a rest from lay.
The concentration of calcium-binding protein (CaBP) and the activities of calcium adenosine triphosphatase (Ca(2+)-ATPase) and carbonic anhydrase (CA) were determined in the shell gland mucosa of hens in two experiments. In Experiment 1, laying hens on a proprietary layer mash were compared with hens rested from lay by the feeding of whole grain barley. In Experiment 2 comparisons were made of laying hens fed the proprietary layer mash and producing eggs with either strong or weak shells. These latter comparisons were also made when the shell gland was quiescent or active with respect to daily eggshell formation. Feeding whole grain barley reduced egg production to zero after 11 days. This reduction in rate of lay was accompanied by significant reductions in all three markers, the effect on Ca(2+)-ATPase and CaBP being less than for CA. Control values were regained between 10 and 16 days after the barley was replaced with the layer mash. Relative shell strength and the physiological status of the shell gland with respect to time of daily eggshell formation had no significant effect on any marker in Experiment 2.
Balnave
D
D
Department of Animal Science, University of Sydney, Camden, New South Wales, Australia.
el-Khatib
N U
NU
Zhang
D
D
D000821:Animal Feed; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002135:Calcium-Binding Proteins / Q000378:metabolism; D000252:Calcium-Transporting ATPases / Q000378:metabolism; D002255:Carbonic Acid / Q000378:metabolism*; D002256:Carbonic Anhydrases / Q000378:metabolism; D002645:Chickens / Q000502:physiology*; D004528:Egg Shell / Q000502:physiology*; D005088:Exocrine Glands / Q000378:metabolism*; D005260:Female; D001467:Hordeum; D009092:Mucous Membrane / Q000378:metabolism; D010058:Oviposition / Q000502:physiology*
D002135:Calcium-Binding Proteins; D002255:Carbonic Acid; D002256:Carbonic Anhydrases; D000252:Calcium-Transporting ATPases; D002118:Calcium