Identification of the classical androgen receptor in male rat liver and prostate cell plasma membranes.
1. Androgen-specific protein have been identified in cell plasma envelopes: gel filtration chromatography, Scatchard and steroid specificity spectrum analyses show the identity of the protein both from liver and ventral prostate plasma membranes (mol. wt 180,000) and nonidentity of the respective cytosol protein receptors. 2. The protein works only at physiological androgen concentration (0.14-14.0 nM of testosterone). 3. The protein possesses 2-orders higher androgen affinity than rat serum testosterone binding globulin (TeBG) and 1-order higher affinity compared to cytosol androgen receptor.
Konoplya
E F
EF
Laboratory of Biochemistry, Republic of Belarus National Academy of Science, Minsk.
Popoff
E H
EH
D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D066298:In Vitro Techniques; D007700:Kinetics; D008099:Liver / Q000378:metabolism*; D008297:Male; D011467:Prostate / Q000378:metabolism*; D051381:Rats; D011944:Receptors, Androgen / Q000302:isolation & purification / Q000378:metabolism*; D012738:Sex Hormone-Binding Globulin / Q000378:metabolism; D013739:Testosterone / Q000378:metabolism
D011944:Receptors, Androgen; D012738:Sex Hormone-Binding Globulin; D013739:Testosterone