title: Culture characteristics of human endometrial glandular epithelium throughout the menstrual cycle: modulation of deoxyribonucleic acid synthesis by 17 beta-estradiol and medroxyprogesterone acetate.
abstract: OBJECTIVE
  The purpose of this study was to evaluate the culture characteristics and cell proliferation of human endometrial glandular epithelial cells in primary culture on extracellular matrix and to evaluate sex steroid modulation of this process.
  
  
  METHODS
  We examined the culture characteristics in 53 endometrial gland preparations obtained throughout the menstrual cycle and determined the incorporation of tritiated thymidine in endometrial epithelial cells as a measure of deoxyribonucleic acid synthesis (cell proliferation) in the presence and absence of 17 beta-estradiol and medroxyprogesterone acetate.
  
  
  RESULTS
  Good culture maintenance of endometrial epithelial monolayers on extracellular matrix was observed from glands derived from proliferate phase endometrium (21 of 23), whereas poor adherence and culture maintenance was observed in all 30 of specimens from the secretory phase. With 17 beta-estradiol treatment for 22 hours, tritiated thymidine incorporation was not different from control, but medroxyprogesterone acetate treatment for 22 hours was associated with diminished tritiated thymidine incorporation by 36% (p < 0.004). When 17 beta-estradiol (10(-8) mol/L) was included in the incubation medium from the time of initial culture, tritiated thymidine incorporation on day 4 was 40% of control (p < 0.006), and tritiated thymidine was not suppressed further by 22 hours of treatment with medroxyprogesterone acetate (10(-7) mol/L). In spite of cellular spread of endometrial epithelial cells to subconfluence, deoxyribonucleic acid content per well did not increase over time in culture.
  
  
  CONCLUSIONS
  We conclude that exposure of endometrial glands to progesterone in vivo inhibits adherence and the establishment of cell monolayers cultured on a thin layer of extracellular matrix. Because 17 beta-estradiol treatment in culture for 22 hours does not increase tritiated thymidine incorporation, it is possible that 17 beta-estradiol exerts its proliferative effect on the endometrial epithelium by an indirect action mediated by stromal cells. The effects of 17 beta-estradiol and medroxyprogesterone acetate on deoxyribonucleic acid synthesis in endometrial epithelial cells may represent an action on a stem cell population of dividing cells or a terminally differentiated cell population that undergoes programmed cell death.
authors: Cecil H. and Ida Green Center for Reproductive Biology Sciences, Obstetrics and Gynecology, University of Texas Southwestern Medical Center, Dallas 75235-9032.
authors: P B
authors: PB
authors: Marshburn
authors: J R
authors: JR
authors: Head
authors: P C
authors: PC
authors: MacDonald
authors: M L
authors: ML
authors: Casey
mesh_terms: D002478:Cells, Cultured; D003094:Collagen; D004247:DNA / Q000096:biosynthesis*; D004338:Drug Combinations; D004717:Endometrium / Q000166:cytology / Q000378:metabolism*; D004847:Epithelial Cells; D004848:Epithelium / Q000378:metabolism; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D006801:Humans; D007797:Laminin; D017258:Medroxyprogesterone Acetate / Q000494:pharmacology*; D008597:Menstrual Cycle; D010969:Plastics; D011509:Proteoglycans; D013936:Thymidine / Q000378:metabolism; D014316:Tritium
chemical_list: D004338:Drug Combinations; D007797:Laminin; D010969:Plastics; D011509:Proteoglycans; D014316:Tritium; C059485:matrigel; D004958:Estradiol; D003094:Collagen; D004247:DNA; D017258:Medroxyprogesterone Acetate; D013936:Thymidine