Effect of hemodialysis on intracellular calcium in human polymorphonuclear neutrophils.
Hemodialysis (HD) leads to neutrophil activation depending on the membrane material used. Calcium plays a major role in PMN function and metabolism. A rise of cytosolic free calcium [Ca2+]i is an important intracellular messenger for neutrophils. In this study we measured intracellular resting Ca2+ and the chemotactic peptide FMLP-stimulated intracellular Ca2+ during HD using membranes made of polysulfone and polymethylmethacrylate (PMMA). Also, we investigated the effect of continuous infusion of nifedipine in a dose of 18 micrograms/kg/h during HD with PMMA. Only HD with PMMA causes an increase of both resting and FMLP-stimulated [Ca2+]i after 30 min of starting HD. Polysulfone did not affect [Ca2+]i. Continuous infusion of nifedipine during HD with PMMA completely inhibited these changes of [Ca2+]i. Although the existence of voltage-sensitive calcium channels has not been proved, the present data provide further indirect evidence either for the existence of calcium channels or for a mechanism of action that is independent of antagonism.
Haag-Weber
M
M
Department of Medicine, University of Saarland, Homburg, FRG.
Mai
B
B
Hörl
W H
WH
D002118:Calcium / Q000097:blood*; D005260:Female; D006801:Humans; D008297:Male; D008567:Membranes, Artificial; D008875:Middle Aged; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism*; D009543:Nifedipine / Q000494:pharmacology; D006435:Renal Dialysis
D008567:Membranes, Artificial; D009543:Nifedipine; D002118:Calcium