Topography of transcription factor complexes on the Saccharomyces cerevisiae 5 S RNA gene.
Locations of component proteins of yeast RNA polymerase III transcription factors (TFIII) A, C and B on a 5 S rRNA gene have been determined by site-specific DNA-protein photo-crosslinking. Comparison with a previously analyzed tRNA gene shows that similar nucleoprotein structures assemble on these two genes despite their differently located internal promoter elements. A principal signature of this homology is the placement of the 95 kDA subunit of TFIIIC, which associates with the box A promoter element of the tRNA gene. On the 5 S rRNA gene, the 95 kDa subunit occupies the same space in the absence of a box A sequence, and despite the presence of a box A-like sequence 30 base-pairs further downstream. A 90 kDa component that was not previously recognized as an integral part of TFIIIC has been specifically located at the 3' end of the 5 S rRNA gene.
Braun
B R
BR
Department of Biology, University of California, San Diego, La Jolla 92093-0634.
Bartholomew
B
B
Kassavetis
G A
GA
Geiduschek
E P
EP
D001483:Base Sequence; D015342:DNA Probes / Q000528:radiation effects; D004275:DNA, Ribosomal / Q000235:genetics / Q000378:metabolism; D005800:Genes, Fungal / Q000235:genetics* / Q000528:radiation effects; D008969:Molecular Sequence Data; D012320:RNA Polymerase III / Q000378:metabolism* / Q000528:radiation effects; D012341:RNA, Ribosomal, 5S / Q000235:genetics*; D012365:RNA, Transfer, Tyr / Q000235:genetics / Q000528:radiation effects; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D035582:Transcription Factor TFIIIA; D035361:Transcription Factor TFIIIB; D014157:Transcription Factors / Q000378:metabolism* / Q000528:radiation effects; D019474:Transcription Factors, TFIII; D014466:Ultraviolet Rays; D000098832:Transcription Factor TFIIIC
D015342:DNA Probes; D004275:DNA, Ribosomal; D012341:RNA, Ribosomal, 5S; D012365:RNA, Transfer, Tyr; D035582:Transcription Factor TFIIIA; D035361:Transcription Factor TFIIIB; D014157:Transcription Factors; D019474:Transcription Factors, TFIII; D000098832:Transcription Factor TFIIIC; D012320:RNA Polymerase III