Determination of acetazolamide in human urine samples by reversed-phase high-performance liquid chromatography in the presence of xanthines.
A simple, rapid and selective high-performance liquid chromatographic assay for the determination of acetazolamide in urine samples is described. After extraction with ethyl acetate, the drug is chromatographed on an HP-Hypersil ODS-C18 column with a mobile phase of acetonitrile-phosphate buffer (pH 3) and ultraviolet detection at 275 nm. The efficiency of the extraction, the linearity and the reproducibility of the method permit the evaluation of acetazolamide urinary excretion a long time after its administration.
Herráez-Hernández
R
R
Departamento de Química Analítica, Facultad de Química, Universidad de Valencia, Burjassot, Spain.
Campíns-Falcó
P
P
Sevillano-Cabeza
A
A
D000086:Acetazolamide / Q000652:urine*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004340:Drug Contamination; D006801:Humans; D014970:Xanthines
D014970:Xanthines; D000086:Acetazolamide