title: The effect of somatostatin analogue SMS 201-995 on serotonin levels in the medium of primary carcinoid cell cultures.
abstract: Carcinoid cell cultures were established from primary tumours and liver and mesenteric metastases. The cells continued to produce serotonin for up to 6 months. Cells from different tumours showed different properties. In most wells carcinoid cells grew on a layer of fibroblasts. The tendency to co-culture seemed to be less marked in cells from liver biopsy specimens. The amount of serotonin decreased to 63% 300 min after addition of the somatostatin analogue SMS 201-995 (SMS) to the culture, compared with controls (p < 0.05; n = 10). This decrease was observed up until 12 days, when SMS was added at each change of medium (p < 0.005; n = 8). In the first 10 min, however, SMS induced an increase in serotonin concentration (p < 0.005; n = 11). This effect may be related to other, immediate stimulatory effects of SMS seen in other cell lines originating from neural ridge-derived tissue. We believe it is important to elucidate the properties of individual tumours, as choice of therapy may vary between patients with the same diagnosis. We have described a method to obtain such information within a couple of days, before a definite treatment is selected.
authors: Medical Dept. A, Rikshospitalet, Oslo, Norway.
authors: M B
authors: MB
authors: Jacobsen
authors: L E
authors: LE
authors: Hanssen
authors: J
authors: J
authors: Bratlie
mesh_terms: D002276:Carcinoid Tumor / Q000378:metabolism* / Q000556:secondary; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism; D015282:Octreotide / Q000494:pharmacology*; D012701:Serotonin / Q000378:metabolism*; D013997:Time Factors; D014407:Tumor Cells, Cultured / Q000378:metabolism
chemical_list: D012701:Serotonin; D015282:Octreotide