Thin-layer chromatographic separation of the ferrochelatase-inhibitory ring A and ring B regioisomers of N-ethylprotoporphyrin from a mixture of the four regioisomers.
When N-alkylprotoporphyrins are prepared synthetically or biologically, a mixture of four regioisomers is obtained. For our studies, separation of the potent ferrochelatase-inhibitory ring A (NA) and ring B (NB) regioisomers from the ring C (NC) and ring D (ND) regioisomers of low potency is required. Previously this separation required two successive high-performance liquid chromatography procedures. We now report that the separation of the zinc complexes of the NA and NB regioisomers from the NC and ND regioisomers can be achieved by a rapid and inexpensive thin-layer chromatography procedure.
Kimmett
S M
SM
Department of Pharmacology and Toxicology, Queen's University, Kingston, Ontario, Canada.
Marks
G S
GS
D000818:Animals; D002855:Chromatography, Thin Layer / Q000379:methods*; D003997:Dicarbethoxydihydrocollidine / Q000008:administration & dosage / Q000031:analogs & derivatives; D005294:Ferrochelatase / Q000037:antagonists & inhibitors*; D007536:Isomerism; D008099:Liver / Q000737:chemistry / Q000187:drug effects; D008297:Male; D011524:Protoporphyrins / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley
D011524:Protoporphyrins; C030826:3,5-dicarbethoxy-2,6-dimethyl-4-ethyl-1,4-dihydropyridine; D003997:Dicarbethoxydihydrocollidine; C030827:N-ethylprotoporphyrin IX; D005294:Ferrochelatase