title: Enkephalin-immunoreactive neurons in the parvicellular subdivisions of the paraventricular nucleus project to the external zone of the median eminence.
abstract: The enkephalin-immunoreactive neurons that project to the external zone of the median eminence were identified on thin paraffin and thick vibratome sections using a combination of retrograde labeling with peripherally administered Fluoro-Gold and immunocytochemistry. The vast majority of the enkephalin-immunoreactive neurons that project to the external zone of the median eminence (ME) reside in the paraventricular nucleus (PVN) of the hypothalamus. Within the PVN, the majority of these hypophysiotropic neurons are located in the medial parvicellular subdivision, while a smaller number can be detected in the anterior and the periventricular subdivisions. Although many enkephalin-immunoreactive neurons are present in other hypophysiotropic areas of the hypothalamus, such as the medial preoptic area, the anterior periventricular area, and the arcuate nucleus, only a few of these can be retrogradely labeled from the ME. These results provide morphological evidence for the key role of paraventricular enkephalin-immunoreactive neurons in the regulation of neuroendocrine functions.
authors: Functional Morphology Section, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709.
authors: I
authors: I
authors: Merchenthaler
mesh_terms: D000818:Animals; D004745:Enkephalins / Q000276:immunology / Q000378:metabolism*; D005456:Fluorescent Dyes; D007150:Immunohistochemistry; D008297:Male; D008473:Median Eminence / Q000166:cytology* / Q000276:immunology; D009434:Neural Pathways / Q000166:cytology; D009474:Neurons / Q000276:immunology / Q000378:metabolism*; D010286:Paraventricular Hypothalamic Nucleus / Q000166:cytology* / Q000276:immunology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013266:Stilbamidines
chemical_list: C049774:2-hydroxy-4,4'-diamidinostilbene, methanesulfonate salt; D004745:Enkephalins; D005456:Fluorescent Dyes; D013266:Stilbamidines