[Establishment and characterization of a human squamous cell carcinoma cell line LK-52 which produce direct activator of coagulant factor X].
Uterus origin squamous cell carcinoma cell line LK-52 was established from surgical specimen of lung metastatic nodule. LK-52 produce poorly differentiated squamous carcinoma in nude mouse, and doubling time in vitro was 38 hours. Chromosome analysis show various abnormality and main mode number was 67 and 68. LK-52 shed active procoagulant substance into culture medium. The culture medium of LK-52 shortening recalcification time of normal human plasma and factor VII or factor IX deficient plasma but not factor X deficient plasma. Procoagulant activity of LK-52 product may induced with direct activation of coagulant factor X. Procoagulant activity which produced by cancer cell may play a important roll in the unbalanced haemostasis of cancer patient.
Inufusa
H
H
Department of the First Surgery, Kinki University School of Medicine, Osaka-sayama, Japan.
Hara
S
S
Mori
N
N
Yasutomi
M
M
D000328:Adult; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D002455:Cell Division; D003546:Cysteine Endopeptidases / Q000378:metabolism* / Q000502:physiology; D005260:Female; D006487:Hemostasis; D006801:Humans; D007621:Karyotyping; D008175:Lung Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D009363:Neoplasm Proteins; D014407:Tumor Cells, Cultured
D009363:Neoplasm Proteins; D003546:Cysteine Endopeptidases; C030910:cancer procoagulant