Immunoelectronmicroscopic study of the nucleoid structure of hydrogen bacteria.
Electron microscopical studies of the nucleoid structure of hydrogen bacteria using ultrahin sections and spread DNA from bacterial cell lysates revealed a different DNA packaging in the cell. A compact state of the major part of DNA at all growth stages and stability of nucleosome-like structures were shown. The use of antibodies to HU protein of E. coli labelled by protein A-colloidal gold demonstrated the immunological relationship between HU protein of E. coli and histone-like proteins of Alcaligenes eutrophus and their possible role in the nucleosome-like DNA packaging in procariotic genome.
Mogilnaya
O A
OA
Institute of Biophysics, Russian Academy of Sciences, Siberian Branch, Krasnoyarsk.
Kiselyova
E V
EV
Medvedeva
S E
SE
Puzir
A P
AP
Guseynov
O A
OA
Kulyba
N N
NN
Kozlov
A V
AV
D000421:Alcaligenes / Q000648:ultrastructure*; D002455:Cell Division; D002467:Cell Nucleus / Q000648:ultrastructure*; D004269:DNA, Bacterial / Q000648:ultrastructure*; D004926:Escherichia coli / Q000648:ultrastructure; D016253:Microscopy, Immunoelectron
D004269:DNA, Bacterial