Noninvasive in vivo percutaneous absorption measurements using X-ray fluorescence.
X-ray fluorescence (XRF) has been used to determine in vivo the percutaneous absorption of 5-iodouracil (5IU) in dimethyl sulfoxide (DMSO) on female Sprague-Dawley rats. An average absorption rate constant of 122 +/- 34 micrograms/cm2-hr was obtained from the XRF measurements on four rats. A comparative study was performed with radiolabeled (125I) 5IU in which the absorption rate constant was determined to be 126 +/- 20 micrograms/cm2-hr. The XRF system described provides a simple, noninvasive means of measuring the percutaneous absorption rate of select compounds by the surface disappearance method.
Robertson
J D
JD
Department of Chemistry, University of Kentucky, Lexington 40506-0055.
Ferguson
E
E
Jay
M
M
Stalker
D J
DJ
D000818:Animals; D005240:Feasibility Studies; D005260:Female; D051381:Rats; D017207:Rats, Sprague-Dawley; D012869:Skin Absorption; D013052:Spectrometry, X-Ray Emission; D014498:Uracil / Q000031:analogs & derivatives* / Q000493:pharmacokinetics
D014498:Uracil; C048747:5-iodouracil