Analysis of differential accumulation of winged bean Kunitz chymotrypsin inhibitor mRNA species by a sequence-specific termination method.
Winged bean Kunitz chymotrypsin inhibitor (WCI) is encoded by a multigene family and accumulation of its mRNA is restricted in mid-maturation stage seeds and tuberous roots. In this paper, we analyzed the accumulation of mRNA derived from each WCI gene using a novel method: sequence-specific termination analysis. The results demonstrated that the accumulation of each WCI mRNA was differentially regulated in winged bean plants.
Umemoto
K
K
Department of Agricultural Chemistry, Hokkaido University, Sapporo, Japan.
Habu
Y
Y
Ohno
T
T
D001483:Base Sequence; D002918:Chymotrypsin / Q000037:antagonists & inhibitors*; D007887:Fabaceae / Q000201:enzymology / Q000235:genetics*; D015870:Gene Expression; D017343:Genes, Plant; D008969:Molecular Sequence Data; D005810:Multigene Family; D010940:Plant Proteins / Q000235:genetics; D010946:Plants, Medicinal; D012333:RNA, Messenger / Q000235:genetics; D013728:Terminator Regions, Genetic; D014158:Transcription, Genetic; D014361:Trypsin Inhibitors / Q000235:genetics*
D010940:Plant Proteins; D012333:RNA, Messenger; D014361:Trypsin Inhibitors; C067097:trypsin inhibitor, Kunitz winged-bean seed; D002918:Chymotrypsin