title: Improved flow cytometric method for HLA-B27 typing.
abstract: HLA-B27 is a cell marker of clinical interest because of its high association with certain diseases. The HLA-B27 antigen was detected on lymphocytes using a monoclonal antibody in an indirect immuno-fluorescence assay using a fluorescence flow cytometer. The considerable crossreaction of the monoclonal antibody with the HLA-B7 antigen was effectively suppressed by masking it by means of human anti-HLA-B7 antiserum. The flow cytometric method was evaluated by comparing the results with those obtained by the standard lymphocytotoxicity test and showed complete agreement in 107 selected patient samples.
authors: Department of Clinical Laboratories, Catharina Hospital, Eindhoven, The Netherlands.
authors: W C
authors: WC
authors: Janssen
authors: J A
authors: JA
authors: Rouwen
authors: J J
authors: JJ
authors: Hoffmann
mesh_terms: D003429:Cross Reactions; D005434:Flow Cytometry / Q000379:methods; D005455:Fluorescent Antibody Technique; D015796:HLA-B27 Antigen / Q000032:analysis*; D015793:HLA-B7 Antigen / Q000032:analysis; D006801:Humans; D010641:Phenotype
chemical_list: D015796:HLA-B27 Antigen; D015793:HLA-B7 Antigen