Determination of eperisone in human plasma by gas chromatography-mass spectrometry.
A gas chromatographic-mass spectrometric method was developed to determine eperisone hydrochloride, 4'-ethyl-2-methyl-3-piperidinopropiophenone hydrochloride, in human plasma over the concentration range 0.2-40 ng/ml. Excellent sensitivity was achieved by selection of a favorable fragment ion, m/z 98, of eperisone and reduction of heat decomposition of eperisone by using a splitless injector and a shortened capillary column. The method described here allows the determination of plasma concentrations as low as 0.2 ng/ml, the concentration attained 6 h after a single oral administration of 50 mg. At eperisone hydrochloride concentrations higher than 0.5 ng/ml, the mean inter-day variation of accuracy of the assay was less than 12%.
Takamatsu
T
T
Department of Clinical Chemistry, Tokyo Research Laboratories, Eisai Co., Ltd., Tokyo, Japan.
Yamazaki
K
K
Kayano
M
M
Takenaka
F
F
Hasui
M
M
Ohkawa
T
T
D002138:Calibration; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D009125:Muscle Relaxants, Central / Q000097:blood*; D011427:Propiophenones / Q000097:blood*; D012016:Reference Values; D015203:Reproducibility of Results
D009125:Muscle Relaxants, Central; D011427:Propiophenones; C030848:eperisone