Application of methionine as a detector molecule for the assessment of oxygen radical generation by human neutrophils and endothelial cells.
Diverse cell types can generate reactive oxygen species (ROS) which are implicated in many disease processes and are ascribed both beneficial and deleterious roles. In vitro studies of this phenomenon indicate that properties of the microenvironment in culture influence the cells' behaviour with regard to ROS generation in vivo. To date, however, the assessment of cellular ROS generation has been limited to techniques which are invasive of the culture environment, or require cells to be in suspension. This study describes the application of NMR spectroscopy to the detection of ROS generation, a technique which is non-invasive of the cell culturing environment.
Stevens
C R
CR
London Inflammation Group, Bone and Joint Research Unit, London Hospital Medical College, UK.
Bucurenci
N
N
Abbot
S E
SE
Sahinoglu
T
T
Blake
D R
DR
Naughton
D
D
Grootveld
M C
MC
D002455:Cell Division / Q000502:physiology; D002478:Cells, Cultured; D003470:Culture Media; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism*; D005609:Free Radicals; D005720:Gamma Rays; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008715:Methionine / Q000031:analogs & derivatives* / Q000032:analysis / Q000528:radiation effects; D008970:Molecular Weight; D009504:Neutrophils / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D015427:Reperfusion Injury / Q000473:pathology; D012996:Solutions
D003470:Culture Media; D005609:Free Radicals; D017382:Reactive Oxygen Species; D012996:Solutions; D008715:Methionine; C013111:methionine sulfoxide