title: Characterization of kallikrein isolated from rat submandibular glands by a simple and rapid purification procedure.
abstract: Numerous biochemical properties (e.g. Mr, carbohydrate content, pI) were determined for kallikrein isolated from rat submandibular glands by a simple, rapid purification procedure. The kinetic behaviour of the enzyme towards various inhibitors and synthetic substrates was investigated. The effects of different salts and detergents on the esterolytic activity of the rat tissue kallikrein were recorded.
authors: Department of Chemistry and Biological Chemistry, University of Essex, Colchester, UK.
authors: T S
authors: TS
authors: el-Thaher
authors: G M
authors: GM
authors: Saed
authors: A A
authors: AA
authors: al-Hamidi
authors: G S
authors: GS
authors: Bailey
mesh_terms: D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives; D003902:Detergents / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007610:Kallikreins / Q000037:antagonists & inhibitors / Q000302:isolation & purification* / Q000378:metabolism; D007700:Kinetics; D008297:Male; D009842:Oligopeptides; D051381:Rats; D012492:Salts / Q000494:pharmacology; D013363:Submandibular Gland / Q000201:enzymology*; D013379:Substrate Specificity; D013696:Temperature
chemical_list: D003902:Detergents; D009842:Oligopeptides; D012492:Salts; C031006:Val-Leu-Arg-p-nitroanilide; D001120:Arginine; D007610:Kallikreins