Quantitative separation of Trichoderma lipid classes on a bonded phase column.
Bond Elut aminopropyl columns were used to purify the different lipid classes of Trichoderma harzianum and Trichoderma viride. This methodology permitted good separation of the fungal lipid classes in less time than traditional techniques. The incorporation of [1 (14)C]linoleic acid into neutral lipids, free fatty acids and phospholipids was quantified for both strains. The fatty acid profile of the different lipid classes of these fungal strains was determined as a function of culture time.
Serrano-Carreon
L
L
ENS.BANA, Laboratoire de Biotechnologie, Université de Bourgogne, Dijon, France.
Hathout
Y
Y
Bensoussan
M
M
Belin
J M
JM
D002845:Chromatography / Q000379:methods*; D005227:Fatty Acids / Q000302:isolation & purification; D008041:Linoleic Acids / Q000302:isolation & purification; D008055:Lipids / Q000302:isolation & purification*; D010743:Phospholipids / Q000302:isolation & purification; D014242:Trichoderma / Q000737:chemistry*
D005227:Fatty Acids; D008041:Linoleic Acids; D008055:Lipids; D010743:Phospholipids