High-resolution imaging of chromosome-related structures by atomic force microscopy.
An atomic force microscope (AFM) was combined with a conventional optical microscope. The optical microscope proved to be very convenient for locating objects of interest. In addition, the high-resolution AFM image can be compared directly with the traditional optical image. The instrument was used to study chromosome structures. High-resolution chromosome images revealed details of the 30-nm chromatide structure, confirming earlier electron microscopic observations. Chromosomes treated with trypsin revealed a banding pattern in height which is very similar to the optical image observed after staining with Giemsa. Furthermore, it is shown that the AFM can be used to locate DNA probes on in situ hybridized chromosomes. Images of the synaptonemal complex isolated from rat spermatocytes revealed details that improve the understanding of the three-dimensional structure of this protein.
De Grooth
B G
BG
Department of Applied Physics, University of Twente, Enschede, The Netherlands.
Putman
C A
CA
D000818:Animals; D002875:Chromosomes / Q000648:ultrastructure*; D006224:Cricetinae; D003412:Cricetulus; D015342:DNA Probes / Q000648:ultrastructure; D008168:Lung / Q000166:cytology; D008297:Male; D008677:Metaphase; D016252:Microscopy, Scanning Tunneling / Q000379:methods*; D051381:Rats; D013090:Spermatocytes / Q000648:ultrastructure
D015342:DNA Probes