Neuroimmune interactions: role for cholinergic neurons in intestinal anaphylaxis.
The role of cholinergic neurons in mediating chloride secretion in anaphylaxis was assessed in muscle-stripped segments of distal colon from guinea pigs immunized to bovine milk. beta-Lactoglobulin evoked a concentration-dependent increase in short-circuit current (Isc) in immune, but not nonimmune, tissues. The Isc response to beta-lactoglobulin was reduced by piroxicam, pyrilamine, and cimetidine. Tetrodotoxin and atropine reduced the Isc response to beta-lactoglobulin in immune animals, whereas mecamylamine and ICS 205-930 were ineffective. beta-Lactoglobulin evoked a concentration-dependent increase in acetylcholine (ACh) release in immune, but not nonimmune, animals. In immune tissues after challenge with beta-lactoglobulin, ACh release paralleled the change in Isc. Piroxicam, cimetidine plus pyrilamine, or a combination of piroxicam, cimetidine, and pyrilamine significantly reduced the release of ACh after beta-lactoglobulin challenge. Histamine, dimaprit, and prostaglandins E2 evoked an increase in ACh release. These results suggest that beta-lactoglobulin releases prostaglandins and histamine probably from mast cells. Secretory responses that occur when immune animals are challenged with beta-lactoglobulin result, in part, from activation of cholinergic neurons that utilize muscarinic synapses for transfer of signals to the epithelium.
Javed
N H
NH
Department of Physiology, Ohio State University, Columbus 43210.
Wang
Y Z
YZ
Cooke
H J
HJ
D000109:Acetylcholine / Q000378:metabolism; D000707:Anaphylaxis / Q000503:physiopathology*; D000818:Animals; D018680:Cholinergic Antagonists; D003106:Colon / Q000276:immunology / Q000378:metabolism / Q000502:physiology; D015232:Dinoprostone / Q000494:pharmacology; D004594:Electrophysiology; D006168:Guinea Pigs; D006632:Histamine / Q000502:physiology; D006633:Histamine Antagonists / Q000494:pharmacology; D007410:Intestinal Diseases / Q000503:physiopathology*; D007782:Lactoglobulins / Q000276:immunology; D008297:Male; D015213:Neuroimmunomodulation / Q000502:physiology*; D009474:Neurons / Q000502:physiology*; D010275:Parasympathetic Nervous System / Q000166:cytology / Q000502:physiology*; D010894:Piroxicam / Q000494:pharmacology; D012702:Serotonin Antagonists; D013779:Tetrodotoxin / Q000494:pharmacology
D018680:Cholinergic Antagonists; D006633:Histamine Antagonists; D007782:Lactoglobulins; D012702:Serotonin Antagonists; D010894:Piroxicam; D013779:Tetrodotoxin; D006632:Histamine; D015232:Dinoprostone; D000109:Acetylcholine