Selective determination, in plasma, of artemether and its major metabolite, dihydroartemisinin, by high-performance liquid chromatography with ultraviolet detection.
A sensitive and selective reversed-phase high-performance liquid chromatographic method for the determination of artemether and its major metabolite dihydroartemisinin in plasma has been developed. It involves extraction of plasma with dichloromethane, solid-phase separation of the two analytes and acid decomposition prior to chromatography on a C18 Spherisorb column with a mobile phase of acetonitrile-water (50:50, v/v). Run time is 30 min. The assay satisfies all of the criteria required for use in clinical pharmacokinetic studies.
Thomas
C G
CG
Department of Pharmacology and Therapeutics, University of Liverpool, UK.
Ward
S A
SA
Edwards
G
G
D000077549:Artemether; D037621:Artemisinins; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D012556:Schistosomicides / Q000097:blood* / Q000493:pharmacokinetics; D012717:Sesquiterpenes / Q000097:blood* / Q000493:pharmacokinetics; D013056:Spectrophotometry, Ultraviolet
D037621:Artemisinins; D012556:Schistosomicides; D012717:Sesquiterpenes; C039060:artenimol; D000077549:Artemether