title: Differential reactivity of Agaricus bisporus lectin with human IgA subclasses in gel precipitation.
abstract: The interaction between purified Agaricus bisporus lectin and several human proteins was studied using the Ouchterlony double diffusion and immunoelectrophoresis techniques. Only one precipitation line was observed with normal human serum, normal human colostrum, IgA1 myeloma serum, both serum monoclonal and secretory IgA1 and monoclonal IgD. No reaction was observed with monoclonal and secretory IgA2, IgG, IgM, alpha 2 macroglobulin or pregnancy-associated alpha 2 glycoprotein. These results were confirmed by hemagglutination inhibition assays when IgA1, IgA2 and IgD were tested. On the basis of this reactivity, ABL could be a useful tool for distinguishing and isolating human IgA subclasses.
authors: Departamento de Bioquimica Clinica, Facultad de Ciencias Quimicas, Universidad Nacional de Córdoba, Argentina.
authors: F J
authors: FJ
authors: Irazoqui
authors: F E
authors: FE
authors: Zalazar
authors: G A
authors: GA
authors: Chiabrando
authors: O
authors: O
authors: Romero
authors: M A
authors: MA
authors: Vides
mesh_terms: D006801:Humans; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D007070:Immunoglobulin A / Q000737:chemistry*; D007132:Immunoglobulin Isotypes / Q000737:chemistry*; D066298:In Vitro Techniques; D037102:Lectins / Q000737:chemistry*; D010835:Phytohemagglutinins / Q000737:chemistry
chemical_list: C041433:Agaricus lectins; D007070:Immunoglobulin A; D007132:Immunoglobulin Isotypes; D037102:Lectins; D010835:Phytohemagglutinins