[Brief counting method of airborne Cryptomeria japonica pollen by a combination of fluorescence antibody staining and flow cytometry].
Airborne pollens collected in a pollen collector (Virtual Impactor) was treated with a fluorescein isothiocyanate-labeled monoclonal antibody (KW-S10) which was strictly specific to Japanese cedar pollen antigen (Cry j I). Flow cytometric analysis revealed that the intensity of fluorescence of the pollen samples treated with the antibody was greater than that of non-treated reference pollen or the antibody treated Hinoki-cypress pollen. By use of this method, it may be possible to display the airborne pollen concentration within 20 min after sampling.
Takahashi
Y
Y
Yamagata Prefectural Institute of Public Health.
Yoshimura
S
S
Masago
H
H
Inouye
K
K
Shirai
T
T
Nagoya
T
T
Sakaguchi
M
M
Inouye
S
S
Katagiri
S
S
D000388:Air / Q000032:analysis*; D000485:Allergens / Q000276:immunology; D000911:Antibodies, Monoclonal; D052179:Antigens, Plant; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D010940:Plant Proteins; D011058:Pollen; D014197:Trees
D000485:Allergens; D000911:Antibodies, Monoclonal; D052179:Antigens, Plant; C065290:Cry j I protein, Cryptomeria japonica; D010940:Plant Proteins