[Hybridization using a digoxigenin-labeled probe for the detection of hepatitis delta virus].
BACKGROUND
The presence of hepatitis delta virus was investigated in liver biopsies by in situ cyto-hybridization with a probe labelled with digoxigenin.


METHODS
The techniques developed included extraction of plasmid DNA by lysis by alkali, electroelution, electrophoresis in agarose gel and digoxigenin labelling, and the application in liver tissue.


RESULTS
Viral RNA was detected in 6 of the 10 patients, and the reactivity was exclusively restricted to the nucleolus of the hepatocytes.


CONCLUSIONS
This method reveals as a sensitive and quick diagnostic procedure, which allows to study the intensity of the infection as well as the serological state of the patient.
Sarría
L
L
Departamento de Microbiología e Inmunología, Facultad de Medicina y Odontología, Universidad del País Vasco.
Gallego
L
L
Lardelli
P
P
Manzano
D
D
Basaras
M
M
Cisterna
R
R
D000208:Acute Disease; D003124:Colorimetry; D004247:DNA / Q000235:genetics; D015342:DNA Probes; D004274:DNA, Recombinant; D003673:Defective Viruses / Q000302:isolation & purification; D004076:Digoxigenin; D006508:Hepatitis Antibodies / Q000097:blood; D006509:Hepatitis B / Q000150:complications; D003699:Hepatitis D / Q000150:complications / Q000175:diagnosis / Q000382:microbiology*; D003698:Hepatitis Delta Virus / Q000276:immunology / Q000302:isolation & purification*; D006521:Hepatitis, Chronic / Q000382:microbiology; D006801:Humans; D017403:In Situ Hybridization; D008103:Liver Cirrhosis / Q000382:microbiology; D009580:Nitroblue Tetrazolium; D010957:Plasmids; D012367:RNA, Viral / Q000032:analysis
D015342:DNA Probes; D004274:DNA, Recombinant; D006508:Hepatitis Antibodies; D012367:RNA, Viral; D009580:Nitroblue Tetrazolium; D004247:DNA; D004076:Digoxigenin
Hibridación con una sonda marcada con digoxigenina para la detección del virus de la hepatitis delta.