title: [The identification of the sites of tRNA(Ser)(GCU) interaction in bovine liver with homologous aminoacyl-tRNA-synthetase by the chemical modification method].
abstract: Interaction of the bovine liver tRNA(GCUSer) having a long variable loop, with the cognate aminoacyl-tRNA synthetase has been studied by alkylation with ethylnitrosourea. It was shown that seryl-tRNA synthetase protects 3'-phosphates of nucleotides 12, 13 in D-stem and 45-47-, 47 G.-, 47 H-variable stem of tRNA(GCUreS) from alkylation. An anticodon loop of tRNA(GCUSer) did not interact with seryl-tRNA synthetase.
authors: L H
authors: LH
authors: Kalachynok
authors: M A
authors: MA
authors: Tukalo
authors: H Kh
authors: HKh
authors: Matsuka
mesh_terms: D000478:Alkylation; D000604:Amino Acyl-tRNA Synthetases / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D001665:Binding Sites; D002417:Cattle; D004347:Drug Interactions; D005038:Ethylnitrosourea / Q000494:pharmacology; D066298:In Vitro Techniques; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D012362:RNA, Transfer, Ser / Q000187:drug effects / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D012362:RNA, Transfer, Ser; D000604:Amino Acyl-tRNA Synthetases; D005038:Ethylnitrosourea
vernacular_title: Vyznachennia dilianok vzaiemodiï tRNK(Ser)(GCU) pechinky byka iz homol'ohichnoiu aminoatsyl-tRNK-syntetazoiu metodom khemichnoï modyfikatsiï.