[Solid phase-sample preparation of ifosfamide and chloroethyl metabolites in biological material. 1. Determination in plasma and urine].
The cytostatic drug ifosfamide (IFF) and its main metabolites 2-dechloroethyl ifosfamide (2-D-IFF) and 3-dechloroethyl ifosfamide (3-D-IFF) were isolated from the plasma matrix with high recovery by solid phase extraction using Bakerbond C18-cartridges. A further cleaning of the extracts is not necessary. An effective separation of IFF and metabolites from interfering compounds present in urine samples prior gc was performed with Extrelut-1 and dichloromethane/isopropanol (95:5, v/v). The described assays may be used for pharmacokinetic studies as well as drug monitoring in the clinical laboratory.
Hennig
B
B
Institut für Pharmazeutische Chemie, Martin-Luther-Universität Halle-Wittenberg.
Rädler
S
S
Blaschke
G
G
Boos
J
J
D006801:Humans; D007069:Ifosfamide / Q000031:analogs & derivatives* / Q000097:blood* / Q000652:urine*
D007069:Ifosfamide
Festphasen-Probenvorbereitung von Ifosfamid und Deschlorethylmetaboliten in biologischem Material. 1. Mitteilung: Bestimmung in Plasma und Urin.