title: Immunologically induced purulent anterior segment inflammation of the guinea pig eye.
abstract: A single conjunctival application of ovalbumin to inbred guinea pigs (IMM/S 209) immunized with the same antigen in Freund's complete adjuvant provoked an acute purulent inflammation of the anterior segment of the eyes with a duration of up to 1 week. Intense conjunctival injection and chemosis were followed by a purulent discharge. A corneal haze was observed regularly, and a considerable proportion of the animals developed a pronounced pannus and corneal ulcers. Tear fluid cytology revealed a rapid increase in cell concentration, from the normal level (less than 10(8)/l) to greater than 10(11)/l. Seventy to 95% of the cells were polymorphonuclear leukocytes. Histological examination revealed an acute inflammatory reaction which radiated from the conjunctival fornices to the entire anterior segments of the eyes. The process was characterized by an intense oedema, vasodilation and perivascular aggregations of polymorphonuclear leukocytes, and to a lesser extent eosinophilic granulocytes which characteristically infiltrated and penetrated the epithelial layers. Neovascularization could be observed early after challenge in the stroma of all parts of the outer eye. Ulcerations of the conjunctival and corneal epithelia were observed frequently. After a number of reiterations of the antigenic challenge, a marked infiltration with lymphocytes and basophils/mast cells was observed, and significant scarring of the conjunctival mucosa developed. In several animals, a slight, but significant co-reaction of the contra-lateral, non-challenged eye was observed.
authors.affiliation: Institute of Medical Microbiology, University of Copenhagen, Denmark.
authors.forename: D
authors.initials: D
authors.lastname: Sompolinsky
authors.forename: L
authors.initials: L
authors.lastname: Lundberg
authors.forename: J U
authors.initials: JU
authors.lastname: Prause
mesh_terms: D000208:Acute Disease; D000818:Animals; D000869:Anterior Eye Segment / Q000276:immunology* / Q000473:pathology; D004195:Disease Models, Animal; D006168:Guinea Pigs; D006969:Hypersensitivity, Immediate / Q000276:immunology; D007637:Keratoconjunctivitis / Q000276:immunology* / Q000473:pathology; D009504:Neutrophils / Q000473:pathology; D013666:Tears / Q000166:cytology