title: Expression of a gene encoding a specific human sperm protein in Chinese hamster ovarian cells.
abstract: The cDNA (RSD-2) encoding a human sperm protein (YWK-II) was isolated from a rat testis lambda gt11 expression library. Its nucleotide sequence was determined. The deduced polypeptide showed high identity with the transmembrane-cytoplasmic domains of A4 amyloid precursor protein of Alzheimer's disease. The RSD-2 was inserted into the EcoRI site of the pSV2-EP vector to construct the pSVRS-2 vector. Chinese hamster ovarian (CHO-dhfr) cells were cotransformed with pSV2-neo and pSVRS-2. mRNA and chromosomal DNA prepared from the transformed cells interacted with [32P]RSD-2 as probe by dot hybridization. The production of the YWK-II protein was determined by staining with the YWK-II mAb by an indirect immunofluorescence technique. There was marked staining of the cytoplasm. The RSD-2 cDNA encoding the YWK-II sperm was expressed in the transformed CHO cells. The pSV2-EP vector and the CHO cell expression system can be utilized to produce sperm proteins for antifertility studies.
authors.affiliation: Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Beijing.
authors.forename: S Y
authors.initials: SY
authors.lastname: Miao
authors.forename: Q Y
authors.initials: QY
authors.lastname: Liu
authors.forename: L F
authors.initials: LF
authors.lastname: Wang
authors.forename: M
authors.initials: M
authors.lastname: Zhao
authors.forename: Y C
authors.initials: YC
authors.lastname: Yan
authors.forename: S S
authors.initials: SS
authors.lastname: Koide
mesh_terms: D016564:Amyloid beta-Protein Precursor; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D001483:Base Sequence; D006224:Cricetinae; D004247:DNA / Q000032:analysis; D005260:Female; D015151:Immunoblotting; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000235:genetics; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins; D010053:Ovary / Q000378:metabolism*; D010957:Plasmids; D012333:RNA, Messenger / Q000032:analysis; D014170:Transformation, Genetic
chemical_list: C489697:APLP1 protein, human; D016564:Amyloid beta-Protein Precursor; D000911:Antibodies, Monoclonal; C498283:Aplp2 protein, rat; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D004247:DNA