Effect of 5-fluorouracil on interleukin-2 expression.
The effect of 5-fluorouracil on the expression of IL-2 gene and the production of IL-2 protein has been studied using spleenic lymphocytes of rats. The IL-2 messenger RNA has been quantitated by dot-blots and IL-2 has been assayed by Con A-blast assay. The results show that there is up to 3.75 fold increase in the production of IL-2 message in 5-fluorouracil treated lymphocytes over Con A treated controls. There is also a concomitant increase in IL-2 protein production upon 5-fluorouracil treatment in rat lymphocytes.
Balaji
K N
KN
Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore.
Nayak
R
R
D000818:Animals; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D005472:Fluorouracil / Q000494:pharmacology*; D015870:Gene Expression / Q000187:drug effects*; D007376:Interleukin-2 / Q000235:genetics*; D007700:Kinetics; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013154:Spleen / Q000166:cytology
D007376:Interleukin-2; D012333:RNA, Messenger; D003208:Concanavalin A; D005472:Fluorouracil