Isolation and characterization of Pseudomonas aeruginosa mutants blocked in the synthesis of pyoverdin.
We have isolated and characterized by chemical and enzymatic analyses three distinct types of pyoverdin-defective (pvd) mutants of Pseudomonas aeruginosa PAO1. The pvd-1 mutant is an L-N5-hydroxyornithine (L-N5-OH-Orn) auxotroph unable to hydroxylate L-ornithine (L-Orn) in a cell-free system and requiring L-N5-OH-Orn for pyoverdin production. The other two types of mutants appear to be blocked in further steps of the biosynthetic pathway leading to pyoverdin, namely, the acylation of L-N5-OH-Orn (pvd-2) and chromophore synthesis (pvd-3). The different pvd mutations were all found to be located in the catA1 region at 47 min of the genetic map of P. aeruginosa PAO1.
Visca
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Istituto di Microbiologia, Università di Roma La Sapienza, Italy.
Serino
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Orsi
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D002874:Chromosome Mapping; D005816:Genetic Complementation Test; D008040:Genetic Linkage; D009154:Mutation; D009842:Oligopeptides; D009952:Ornithine / Q000378:metabolism; D010860:Pigments, Biological / Q000096:biosynthesis*; D011550:Pseudomonas aeruginosa / Q000235:genetics / Q000378:metabolism*
D009842:Oligopeptides; D010860:Pigments, Biological; C042453:pyoverdin; D009952:Ornithine