title: The characterization of two new low molecular weight proteins (LMPs) from Streptococcus pyogenes.
abstract: Two novel extracellular mitogenic substances were isolated from Streptococcus pyogenes strain NY-5 and characterized. The purification steps involved an initial enrichment of the proteins from culture supernatant by silica gel adsorption, followed by ion exchange chromatography and gel filtration. The purified materials were homogeneous in SDS-PAGE, showed estimated molecular weights of 12 kD and isoelectric points of 4.7 and 4.3, respectively. Both proteins (LMP-12k-4.3pI and LMP-12k-4.7pI) demonstrated lymphocyte transformation activity at a concentration of 0.1 microgram/ml. The LMP-12k-4.7pI showed a 69.2% homology of the amino acid sequence with that of a phosphocarrier protein of Staphylococcus aureus and with a total identity in the active centre. The same protein was also isolated from streptococcal group C strain H46A with an N-terminal amino acid sequence being identical. The LMP-12k-4.7pI demonstrated biochemical properties identical with those of the earlier described streptococcal pyrogenic exotoxin type D. The LMP-12k-4.3pI did not show such a clear relation to other functional proteins.
authors.affiliation: Institute of Microbiology and Experimental Therapy, Jena, Germany.
authors.forename: D
authors.initials: D
authors.lastname: Gerlach
authors.forename: H
authors.initials: H
authors.lastname: Alouf
authors.forename: L
authors.initials: L
authors.lastname: Morávek
authors.forename: M
authors.initials: M
authors.lastname: Pavlik
authors.forename: W
authors.initials: W
authors.lastname: Köhler
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects; D008934:Mitogens / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D008969:Molecular Sequence Data; D008970:Molecular Weight; D016415:Sequence Alignment; D013297:Streptococcus pyogenes / Q000737:chemistry* / Q000254:growth & development; D013879:Thioredoxins / Q000737:chemistry
chemical_list: D001426:Bacterial Proteins; D008934:Mitogens; D013879:Thioredoxins