[Resistance to antibiotics of vibrio cholerae strains isolated in Angola].
Among 87 strains of Vibrio choleare (78 Ogawa serotype and 9 Inaba serotype strains) isolated in Angola in 1987-1990, 86% exhibited multiple resistance to antimicrobials. Eighty-four to 86% of strains were resistant to ampicillin with beta-lactamase production (MIC greater than or equal to 512 mg/l), streptomycin (MIC greater than or equal to 64 mg/l), spectinomycin (MIC greater than or equal to 1,024 mg/l), and trimethoprime-sulfisoxazole (MIC greater than 1,024 mg/l). Seventy-four per cent of strains were resistant to kanamycin (MIC = 512 mg/l), 26% to chloramphenicol (MIC = 32 mg/l), 10% to tetracycline (MIC = 16 mg/l), and 10% to gentamycin (MIC greater than or equal to 32 mg/l). Transfer to E. coli K12 was associated with a substantial increase in expression of resistance to tetracycline and chloramphenicol (CAT type I), with MICs in the 128-512 mg/l range. Transfer rates to E. coli K12 of plasmids for the various resistance phenotypes were 10(-6)/10(-8). The size of the isolated plasmids was 100 Md in diameter and belonged to the incompatibility group inc 6-C.
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Centro Nac. Invest. Cientifica, Luanda.
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D000810:Angola; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002701:Chloramphenicol / Q000494:pharmacology; D002702:Chloramphenicol Resistance; D002771:Cholera / Q000382:microbiology; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics; D006801:Humans; D066298:In Vitro Techniques; D010957:Plasmids / Q000235:genetics; D013424:Sulfanilamides / Q000494:pharmacology; D013752:Tetracycline / Q000494:pharmacology; D013753:Tetracycline Resistance; D014162:Transfection / Q000502:physiology; D014734:Vibrio cholerae / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification
D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D013424:Sulfanilamides; D002701:Chloramphenicol; D013752:Tetracycline
Résistance aux antibiotiques de souches de Vibrio cholerae isolées en Angola.