title: Alloantigen recognition by two human natural killer cell clones is associated with HLA-C or a closely linked gene.
abstract: Human natural killer (NK) cells with the CD3- CD16+ phenotype recognize allospecificities on normal T-cell blasts. The NK-defined specificity 1 (NK-1) is recessively inherited and has been mapped to the major histocompatibility complex between the complement gene cluster and HLA-A. A gene for NK-1, however, has not been identified. Here we demonstrate that NK-1 and the recently defined NK specificity 2 (NK-2) are reciprocally associated with homozygosity for a diallelic polymorphism at amino acid positions 77 and 80 in the putative peptide-binding site of HLA-C (P less than 10(-5)). NK-cell recognition of allogeneic cells may, therefore, be controlled by HLA-C itself or by a closely linked gene(s), which dominantly prevents (resistance alleles) or recessively permits (susceptibility alleles) recognition of still-unknown target determinants.
authors.affiliation: Division of Tumor Virology, Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA 02115.
authors.forename: M
authors.initials: M
authors.lastname: Colonna
authors.forename: T
authors.initials: T
authors.lastname: Spies
authors.forename: J L
authors.initials: JL
authors.lastname: Strominger
authors.forename: E
authors.initials: E
authors.lastname: Ciccone
authors.forename: A
authors.initials: A
authors.lastname: Moretta
authors.forename: L
authors.initials: L
authors.lastname: Moretta
authors.forename: D
authors.initials: D
authors.lastname: Pende
authors.forename: O
authors.initials: O
authors.lastname: Viale
mesh_terms: D001483:Base Sequence; D002874:Chromosome Mapping; D005805:Genes, MHC Class I; D008040:Genetic Linkage; D015236:HLA-C Antigens / Q000276:immunology*; D006239:Haplotypes; D006801:Humans; D007694:Killer Cells, Natural / Q000276:immunology*; D008285:Major Histocompatibility Complex; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D016133:Polymerase Chain Reaction
chemical_list: D015236:HLA-C Antigens; D009838:Oligodeoxyribonucleotides