Polypeptides associated with Pasteurella multocida infection in rabbits.
Polypeptides from whole cell preparations of Pasteurella multocida serotypes A:12 and A:3 were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to nitrocellulose paper. Antigens were detected by immunoblot analysis, using sera from 3 groups of rabbits. Sera were obtained from rabbits inoculated intranasally with P multocida serotype A:12 or A:3, from rabbits maintained in a rabbitry with enzootic P multocida A:12 infection, and from rabbits maintained in a rabbitry with enzootic P multocida A:3 infection. Immunoblot analyses of pre- and postinoculation sera from experimentally infected rabbits, using serotype A:12 antigen, revealed 3 polypeptides with approximate molecular mass of 28, 30, and 37 kDa that consistently detected antibodies after P multocida-induced infection. Sera from rabbits naturally infected with either serotype, tested against serotype A:12 and A:3 antigens, detected the same polypeptides in both serotypes. Thus, immunologic reactivity to these polypeptides may be useful for serologic detection of P multocida infection.
Zimmerman
T E
TE
Department of Comparative Medicine, School of Medicine, University of Washington, Seattle 98195.
Deeb
B J
BJ
DiGiacomo
R F
RF
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D000942:Antigens, Bacterial / Q000032:analysis* / Q000276:immunology; D001426:Bacterial Proteins / Q000032:analysis / Q000276:immunology; D001428:Bacterial Vaccines; D003720:Densitometry; D004591:Electrophoresis, Polyacrylamide Gel; D015151:Immunoblotting; D010326:Pasteurella Infections / Q000175:diagnosis / Q000517:prevention & control / Q000662:veterinary*; D016979:Pasteurella multocida / Q000737:chemistry / Q000276:immunology*; D010455:Peptides / Q000032:analysis* / Q000276:immunology; D011817:Rabbits
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D010455:Peptides