Fibrinogen-degrading proteins from Haemonchus contortus used to vaccinate sheep.
Sixteen nonsibling sheep, approximately 12 months old, that were raised in a helminth-free environment, were used for 2 protection studies 6 months apart. Sheep were vaccinated weekly for 5 weeks by IM injection of fibrinogen-degrading proteins derived from the intestinal tract of adult Haemonchus contortus. Ten days after the last vaccination, sheep were given 2,500 infective H contortus larvae by intraruminal injection. Vaccinated sheep produced specific antibodies, and were protected from the worm challenge. Significant differences in mean fecal worm egg counts for 56 days after worm challenge, in mean numbers of H contortus worms, and female fecundity ratios at necropsy were detected in vaccinated sheep, compared with those in control sheep. These data suggest that the fibrinogen-degrading proteins have a protective role in vaccination of sheep against H contortus.
Boisvenue
R J
RJ
Department of Animal Health Discovery Research, Lilly Research Laboratories, Division of Eli Lilly & Co., Greenfield, IN 46140.
Stiff
M I
MI
Tonkinson
L V
LV
Cox
G N
GN
Hageman
R
R
D000018:Abomasum / Q000469:parasitology; D000818:Animals; D005243:Feces / Q000469:parasitology; D005260:Female; D005298:Fertility; D005340:Fibrinogen / Q000378:metabolism; D006188:Haemonchiasis / Q000517:prevention & control / Q000662:veterinary*; D006189:Haemonchus / Q000276:immunology*; D015801:Helminth Proteins / Q000276:immunology*; D010270:Parasite Egg Count / Q000662:veterinary; D012756:Sheep; D012757:Sheep Diseases / Q000517:prevention & control*; D014611:Vaccination / Q000662:veterinary*
D015801:Helminth Proteins; D005340:Fibrinogen