title: Enterohemorrhagic Escherichia coli associated with hemolytic-uremic syndrome in Chilean children.
abstract: A clinicoepidemiological study was undertaken to determine if enterohemorrhagic Escherichia coli (EHEC) was associated with hemolytic-uremic syndrome (HUS) in children in Santiago, Valdivia, and Temuco, Chile. Prospective surveillance detected 20 hospitalized cases of HUS in children less than 4 years of age in these cities from March 1988 to March 1989. Each HUS patient was matched (by sex and age) with two control children (hospitalized elective-surgery patients). To detect EHEC, DNA from stool culture isolates of E. coli was detected by hybridization with biotin-labelled DNA probes specific for the EHEC virulence plasmid, Shiga-like toxin I (SLT-I) or SLT-II. Stool cultures from 6 of 20 cases (30%) and from 2 of 38 controls (5.3%) yielded EHEC (P = 0.0158). EHEC isolates from all HUS cases hybridized with the EHEC plasmid probe and with probes for SLT-I or -II (or both). The serogroups of the isolates included O157, O26, and O111. EHEC causes HUS in Chile, and the biotinylated gene probes are practical diagnostic tools for epidemiologic studies.
authors.affiliation: Microbiology Unit, Faculty of Medicine, University of Chile, Santiago.
authors.forename: A
authors.initials: A
authors.lastname: Cordovéz
authors.forename: V
authors.initials: V
authors.lastname: Prado
authors.forename: L
authors.initials: L
authors.lastname: Maggi
authors.forename: J
authors.initials: J
authors.lastname: Cordero
authors.forename: J
authors.initials: J
authors.lastname: Martinez
authors.forename: A
authors.initials: A
authors.lastname: Misraji
authors.forename: R
authors.initials: R
authors.lastname: Rios
authors.forename: G
authors.initials: G
authors.lastname: Soza
authors.forename: A
authors.initials: A
authors.lastname: Ojeda
authors.forename: M M
authors.initials: MM
authors.lastname: Levine
mesh_terms: D001427:Bacterial Toxins / Q000235:genetics; D002675:Child, Preschool; D002677:Chile; D015342:DNA Probes; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D006463:Hemolytic-Uremic Syndrome / Q000209:etiology / Q000382:microbiology*; D006801:Humans; D022622:Shiga Toxin 1; D022641:Shiga Toxin 2; D014774:Virulence / Q000235:genetics
chemical_list: D001427:Bacterial Toxins; D015342:DNA Probes; D004269:DNA, Bacterial; D022622:Shiga Toxin 1; D022641:Shiga Toxin 2