title: Adrenal and circulating renin-angiotensin system in stroke-prone hypertensive rats.
abstract: The plasma and adrenal renin-angiotensin system in stroke-prone spontaneously hypertensive rats (SHRSP) and Wistar-Kyoto (WKY) rats were examined in animals at 5, 11, 18, and 25 weeks of age. Plasma active renin was significantly increased in 18- and 25-week-old SHRSP with impaired renal function, whereas there was no difference in the plasma prorenin level or renal renin content between the two strains at all ages examined. Thus, the rate of activation of prorenin seems to be enhanced in the kidney of SHRSP with malignant hypertension. Adrenal renin contents were severalfold higher in SHRSP than WKY rats at all ages. However, adrenal angiotensin peptides were not increased in SHRSP aged 5 and 11 weeks. In 18-week-old SHRSP, adrenal angiotensin II (Ang II) and III (Ang III) levels were fourfold and 1.8-fold higher, respectively, than in WKY rats, accompanied by 1.5-fold higher plasma aldosterone. Increased adrenal angiotensin and plasma aldosterone were also found in 25-week-old SHRSP. Zonal distribution studies indicated that the elevated Ang II and III in SHRSP were derived mainly from the capsular tissue (the zona glomerulosa). To examine the contribution of circulating angiotensin to the adrenal angiotensin content, effects of bilateral nephrectomy on adrenal angiotensin and renin were examined in 18-week-old rats. At 24 hours after nephrectomy, plasma angiotensin, prorenin, and active renin were decreased to almost negligible concentrations. Conversely, in both adrenal capsular and decapsular tissues of SHRSP and WKY rats, neither angiotensin nor renin was significantly decreased after nephrectomy. These results suggest that the increase in adrenal capsular Ang II contents in SHRSP may be partly due to an enhanced local production of Ang II.
authors.affiliation: Department of Pharmacology, Osaka City University Medical School, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Kim
authors.forename: M
authors.initials: M
authors.lastname: Tokuyama
authors.forename: M
authors.initials: M
authors.lastname: Hosoi
authors.forename: K
authors.initials: K
authors.lastname: Yamamoto
mesh_terms: D000311:Adrenal Glands / Q000378:metabolism / Q000503:physiopathology*; D000450:Aldosterone / Q000378:metabolism; D000804:Angiotensin II / Q000037:antagonists & inhibitors / Q000097:blood* / Q000378:metabolism; D000818:Animals; D002561:Cerebrovascular Disorders / Q000209:etiology*; D003208:Concanavalin A / Q000378:metabolism; D004792:Enzyme Precursors / Q000097:blood; D006973:Hypertension / Q000378:metabolism; D007106:Immune Sera / Q000502:physiology; D008297:Male; D009392:Nephrectomy; D011863:Radioimmunoassay; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D012083:Renin / Q000097:blood* / Q000378:metabolism; D012084:Renin-Angiotensin System
chemical_list: D004792:Enzyme Precursors; D007106:Immune Sera; D003208:Concanavalin A; D000804:Angiotensin II; D000450:Aldosterone; D012083:Renin