Augmented expression of HSP72 protein in normal human fibroblasts irradiated with ultraviolet light.
Normal human fibroblasts synthesized heat shock protein (HSP) 72 constitutively and its expression was augmented 6 hours after UV irradiation. Maximum induction of HSP72 was obtained at 12 hours and HSP72 showed a punctuated distribution in nucleus. While unscheduled DNA synthesis was almost completed 12 hours after UV irradiation, the S phase fraction decreased immediately and recovered after 6 hours. Thus, HSP72 augmentation was occurred coincidentally with the recovery of S phase, and suggested that HSP72 had some function during the recovery of DNA replication inhibited after UV irradiation.
Suzuki
K
K
Division of Radiation Biology, School of Medicine, Yokohama City University, Japan.
Watanabe
M
M
D015153:Blotting, Western; D002453:Cell Cycle / Q000528:radiation effects; D002467:Cell Nucleus / Q000378:metabolism / Q000648:ultrastructure; D002478:Cells, Cultured; D004261:DNA Replication / Q000528:radiation effects; D004622:Embryo, Mammalian; D005347:Fibroblasts / Q000378:metabolism / Q000528:radiation effects; D005455:Fluorescent Antibody Technique; D006360:Heat-Shock Proteins / Q000096:biosynthesis* / Q000302:isolation & purification; D006801:Humans; D007700:Kinetics; D014466:Ultraviolet Rays
D006360:Heat-Shock Proteins