Genetic analysis of fingerprints in Mérinos d'Arles x Booroola Merino crossbred sheep.
The M13.13 minisatellite probe, consisting of a polymer of the M13 VNTR consensus sequence, cross-hybridized to ovine DNA and allowed detection of several polymorphic loci. Individual specific patterns were obtained in sheep using this probe. Pedigree analysis showed that individuals were heterozygous for most of the DNA fragments detected (88%). By studying the segregation of male's variable DNA fragments, a minimum of 10 loci were defined. The ovine DNA 'fingerprint' obtained with M13.13 is polymorphic enough to be used efficiently in animal identification, paternity testing, and possibly as a source of genetic markers for linkage analysis.
Lanneluc
I
I
INRA Centre de Recherches de Toulouse, Laboratoire de Génétique Cellulaire, Castanet-Tolosan, France.
Hospital
F
F
Chevalet
C
C
Elsen
J M
JM
Gellin
J
J
D000818:Animals; D001483:Base Sequence; D003433:Crosses, Genetic; D016172:DNA Fingerprinting; D004276:DNA, Satellite; D005260:Female; D008040:Genetic Linkage; D008297:Male; D008969:Molecular Sequence Data; D009841:Oligonucleotides; D010375:Pedigree; D011110:Polymorphism, Genetic; D012756:Sheep / Q000235:genetics*
D004276:DNA, Satellite; D009841:Oligonucleotides