Relationship between selected bacteria and the growth of immature house flies, Musca domestica, in an axenic test system.
To investigate the relationship between immature (maggot) house flies, Musca domestica, and bacteria, we compared the development of sterile first-instar maggots in each of 10 pure blood agar cultures of bacteria with growth on sterile blood agar (negative control) and on standard house fly rearing medium (positive control). Nine species of bacteria representing gram-negative and gram-positive rods, coccoid, and micrococcoid cell types supported house fly growth on blood agar. One bacterium, a strain of Bacillus cereus, inhibited maggot growth. The percent pupation for maggots that developed in the presence of eight of nine bacteria (range, 41-69%), was significantly greater than in sterile blood agar (0-5%), and did not differ significantly from maggot growth in the rearing medium (50-90%). Average pupal weight for maggots that developed on blood agar with bacteria ranged from 19 to 21 mg, a reflection of favorable growth conditions. Average pupal weight in the presence of three bacteria (19.9, 19.4, and 19.4 mg) was significantly less than respective pupal weights in house fly rearing medium (24.0, 22.3, and 22.1 mg), but there was no difference in average pupal weight with six bacteria and the house fly rearing medium. These findings illustrate that bacteria or their metabolic products are essential as nutrients for house fly maggot growth in blood agar; a wide variety of adventitious bacteria can contribute to the suitability of an organic substrate for maggot growth; and a naturally occurring isolate of B. cereus limits house fly maggot growth in blood agar, a relationship that has not been reported previously.
Schmidtmann
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Livestock Insects Laboratory, USDA-ARS, Beltsville Agricultural Research Center, Md. 20705.
Martin
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D000818:Animals; D001409:Bacillus cereus / Q000502:physiology; D018407:Bacterial Physiological Phenomena; D004926:Escherichia coli / Q000502:physiology; D006793:Houseflies / Q000254:growth & development / Q000382:microbiology*; D007711:Klebsiella pneumoniae / Q000502:physiology; D007814:Larva / Q000254:growth & development / Q000382:microbiology; D008837:Micrococcus / Q000502:physiology; D013210:Staphylococcus / Q000502:physiology