title: Investigation of how different filters affect some biochemical properties of stored platelet concentrates.
abstract: The aim of the present study was to investigate how four different filters, i.e. Imugard IG500 (Terumo, Japan), Miropore (Miramed, Italy), Pall P1-100 (Pall, USA) and Sepacell P1-10A (Asahi, Japan) affect some biochemical properties of platelet concentrates. The work was conducted using 42 pairs of platelet concentrates. After 2 days of storage, one of the preparations was filtered and the other served as an unfiltered control. Immediately after filtration, determination of the platelet count, desarginated activated complement factor 3 (C3a des arg) and the extracellular and total concentrations of platelet factor 4 (PF4) and lactate dehydrogenase (LDH) were carried out on both these platelet concentrates. After an additional storage period of 3 d, extracellular concentrations of PF4 and LDH were determined on both concentrates. A significant decrease of extracellular PF4 concentration was found immediately after filtration when Pall P1-100 and Imugard IG500 were used. During the 3-d storage after filtration, the concentrates filtered with Imugard IG500 and Pall P1-100 demonstrated significantly higher platelet lysis as compared to the unfiltered controls. It is concluded that the present work demonstrates storage instability after filtration with Imugard IG500 and Pall P1-100. Therefore, platelet concentrates filtered with these filters would not appear to be suitable for storage.
authors.affiliation: Department of Transfusion Medicine, Orebro Medical Center Hospital, Sweden.
authors.forename: P
authors.initials: P
authors.lastname: Järemo
authors.forename: J
authors.initials: J
authors.lastname: Kutti
mesh_terms: D001792:Blood Platelets / Q000502:physiology*; D001793:Blood Preservation; D002469:Cell Separation / Q000295:instrumentation*; D015926:Complement C3a / Q000378:metabolism; D005374:Filtration / Q000295:instrumentation*; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000097:blood; D010976:Platelet Count; D010978:Platelet Factor 4 / Q000378:metabolism
chemical_list: C044541:complement C3a, des-Arg-(77)-; D010978:Platelet Factor 4; D015926:Complement C3a; D007770:L-Lactate Dehydrogenase