[Simple method for measurement of erythrocyte osmotic fragility].
We developed a simple and quantitative method for determination of erythrocyte osmotic fragility, which is based on a logarithmic linearization of osmotic fragility curve and a calculation of three parameters, that is, C50 (the osmolarity causing 50% hemolysis), beta (the slope of the curve) and H (% hemolysis before treatment). By making use of personal computer program, calculation of the parameters and print-out of reports are automated. As the results, it takes only 30 min to finish all procedure. This method is very useful particularly for the laboratory screening of hereditary spherocytosis.
Ideguchi
H
H
Department of Clinical Chemistry and Laboratory Medicine, Fukuoka University Hospital.
Shinohara
K
K
Ishikawa
A
A
Futata
Y
Y
Sugisaki
S
S
Hamasaki
N
N
D006801:Humans; D008838:Microcomputers; D009996:Osmotic Fragility; D012016:Reference Values; D012984:Software; D013103:Spherocytosis, Hereditary / Q000175:diagnosis*