title: Expression of a single dopamine transporter cDNA can confer two cocaine binding sites.
abstract: Radiolabeled cocaine analogs can bind to low and high affinity sites on striatal dopamine transporters (DAT). Recently, a cDNA encoding a rat brain dopamine transporter pDAT1 has been cloned. COS cells transfected with the pDAT1 in a eukaryotic expression vector express both a high (KD = 3.4 nM) and low affinity (KD = 163.6 nM) cocaine binding sites, suggesting that both sites are provided by a single gene product.
authors.affiliation: Laboratory of Molecular Pharmacology, National Institute on Drug Abuse, Baltimore, MD.
authors.forename: J W
authors.initials: JW
authors.lastname: Boja
authors.forename: L
authors.initials: L
authors.lastname: Markham
authors.forename: A
authors.initials: A
authors.lastname: Patel
authors.forename: G
authors.initials: G
authors.lastname: Uhl
authors.forename: M J
authors.initials: MJ
authors.lastname: Kuhar
mesh_terms: D000818:Animals; D001665:Binding Sites / Q000502:physiology; D002352:Carrier Proteins / Q000235:genetics*; D002460:Cell Line; D003001:Cloning, Molecular; D003042:Cocaine / Q000031:analogs & derivatives* / Q000378:metabolism; D003342:Corpus Striatum / Q000378:metabolism; D004247:DNA / Q000096:biosynthesis*; D050483:Dopamine Plasma Membrane Transport Proteins; D008562:Membrane Glycoproteins; D026901:Membrane Transport Proteins; D008566:Membranes / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000235:genetics*; D011869:Radioligand Assay; D051381:Rats; D011955:Receptors, Drug / Q000235:genetics*
chemical_list: D002352:Carrier Proteins; D050483:Dopamine Plasma Membrane Transport Proteins; D008562:Membrane Glycoproteins; D026901:Membrane Transport Proteins; D009419:Nerve Tissue Proteins; D011955:Receptors, Drug; C493216:Slc6a3 protein, rat; C061445:cocaine receptor; C021279:(1R-(exo,exo))-3-(4-fluorophenyl)-8-methyl-8- azabicyclo(3.2.1)octane-2-carboxylic acid, methyl ester; D004247:DNA; D003042:Cocaine