Rapid separation of creatine, phosphocreatine and adenosine metabolites by ion-pair reversed-phase high-performance liquid chromatography in plasma and cardiac tissue.
A rapid ion-pair reversed-phase high-performance liquid chromatographic method has been developed for the simultaneous detection of creatine, phosphocreatine, hypoxanthine, inosine, adenosine, AMP, ADP, ATP, 8-azaguanine, 2-chloroadenosine, and 2'-O-methyladenosine. This method has proven useful for measuring changes in nucleotide concentrations in both heart tissue and plasma samples. Separation of the compounds of interest is achieved in less than 8 min with re-equilibration in 7 min, making the total run time 15 min. Separation is performed on a 3-microns Ultrasphere ODS column employing tetrabutylammonium phosphate as the ion-pair agent and dipotassium hydrogenphosphate as the counter ion. The accuracy, rapid separation, and re-equilibration time make this method particularly useful for the routine analysis of a large number of samples.
Scott
M D
MD
Department of Anesthesiology, St. Louis University, MO 63110-0250.
Baudendistel
L J
LJ
Dahms
T E
TE
D000241:Adenosine / Q000097:blood / Q000302:isolation & purification*; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003401:Creatine / Q000097:blood / Q000302:isolation & purification*; D004285:Dogs; D009206:Myocardium / Q000737:chemistry*; D010725:Phosphocreatine / Q000097:blood / Q000302:isolation & purification*; D011817:Rabbits; D012015:Reference Standards; D013056:Spectrophotometry, Ultraviolet
D010725:Phosphocreatine; D000241:Adenosine; D003401:Creatine