title: Nucleotide sequence of Mycoplasma mycoides subspecies Mycoides plasmid pKMK1.
abstract: To facilitate the development of mycoplasmal cloning vectors, we have determined the nucleotide sequence of pKMK1, a cryptic plasmid isolated from Mycoplasma mycoides subsp. mycoides. It is 1875 bp in length and contains two open reading frames (ORFs) that share homology with ORFs from members of a large family of gram-positive bacterial plasmids which replicate via a single-stranded DNA intermediate. Putative origins of replication and candidate cloning sites have been identified.
authors.affiliation: Department of Microbiology, University of Alabama, Birmingham 35294.
authors.forename: K W
authors.initials: KW
authors.lastname: King
authors.forename: K
authors.initials: K
authors.lastname: Dybvig
mesh_terms: D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000235:genetics; D001483:Base Sequence; D004277:DNA, Single-Stranded / Q000235:genetics; D014644:Genetic Variation; D008969:Molecular Sequence Data; D009176:Mycoplasma mycoides / Q000235:genetics*; D009690:Nucleic Acid Conformation; D010957:Plasmids / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
chemical_list: D004277:DNA, Single-Stranded