Single-step method for purification of human transferrin from a by-product of chromatographic fractionation of plasma.
A rapid, simple and convenient method is described for the isolation, on a pilot scale, of pure and functional human transferrin from an unexploited by-product of chromatographic fractionation of plasma. In a single chromatographic step on DEAE-Spherodex, 97% pure transferrin was obtained in 75% yield. A virus inactivation treatment was included in the preparative process in order to guarantee the safety of the final product, which could be used in culture media.
Rivat
C
C
Unité INSERM U 284, Vandoeuvre-les-Nancy, France.
Sertillanges
P
P
Patin
E
E
Stoltz
J F
JF
D002852:Chromatography, Ion Exchange; D002853:Chromatography, Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007501:Iron / Q000032:analysis; D012710:Serum Albumin, Bovine / Q000032:analysis; D014168:Transferrin / Q000302:isolation & purification*; D014780:Viruses / Q000737:chemistry
D014168:Transferrin; D012710:Serum Albumin, Bovine; D007501:Iron