Hepatic lesions caused by excretory and secretory products of Ascaris lumbricoides in golden hamster.
OBJECTIVE
To ascertain the role of excretory and secretory (ES) products of Ascaris lumbricoides in liver damage.


METHODS
The ES products of A lumbricoides were collected in vitro and their SDS-PAGE analysis was done. Feeding and subcutaneous injection of ES products were done in hamsters. Estimation of serum proteins, alkaline phosphatase and alanine aminotransferase and histology of liver were carried out. Control animal experiments were done concurrently.


RESULTS
The ES products of A lumbricoides contained several proteins ranging in molecular weight from 14 to 205 Kd. Prolonged feeding of ES products caused elevation of ALT and amyloid deposition in the liver, whereas short term feeding or subcutaneous challenge caused focal cell necrosis and granuloma formation in the liver.


CONCLUSIONS
ES products of A lumbricoides can produce liver damage.
Mazumder
D N
DN
Department of Gastroenterology, Institute of Post-Graduate Medical Education and Research, Calcutta.
Santra
A
A
Dutta
G
G
Ghosh
N
N
Chowdhury
M K
MK
D000686:Amyloidosis / Q000469:parasitology; D000818:Animals; D000947:Antigens, Helminth / Q000009:adverse effects*; D001196:Ascariasis / Q000473:pathology*; D001200:Ascaris / Q000276:immunology*; D006224:Cricetinae; D004591:Electrophoresis, Polyacrylamide Gel; D006099:Granuloma / Q000469:parasitology; D008099:Liver / Q000382:microbiology / Q000473:pathology*; D008109:Liver Diseases, Parasitic / Q000473:pathology*; D008297:Male; D008647:Mesocricetus
D000947:Antigens, Helminth