Tat- and Rev-directed antisense RNA expression inhibits and abolishes replication of human immunodeficiency virus type 1: a temporal analysis.
Replication of human immunodeficiency virus type 1 (HIV-1) was inhibited by stable intracellular expression of antisense RNA in the human T-lymphoid cell line Jurkat. When the viral subregion encoding the HIV-1 activator proteins was targeted, the extent of antisense RNA-mediated inhibition was greater than 97% during the first 2 weeks postinfection. Later in the time course, productive HIV-1 infection broke through at high initial infective doses. However, at initial multiplicities of infection equal to or smaller than 0.1, HIV-1 production was not detectable during the 5 weeks of observation. The results underline the effectiveness of stable intracellular antisense RNA expression in inhibiting HIV-1 replication.
Sczakiel
G
G
Forschungsschwerpunkt Angewandte Tumorvirologie, Deutsches Krebsforschungszentrum, Heidelberg, Germany.
Oppenländer
M
M
Rittner
K
K
Pawlita
M
M
D001483:Base Sequence; D002460:Cell Line; D015967:Gene Expression Regulation, Viral; D015757:Genes, rev; D015756:Genes, tat; D015497:HIV-1 / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D008221:Lymphoid Tissue; D008969:Molecular Sequence Data; D016372:RNA, Antisense / Q000494:pharmacology*; D013601:T-Lymphocytes; D013997:Time Factors; D014779:Virus Replication / Q000187:drug effects*
D016372:RNA, Antisense