Quantification of erythrocyte S-adenosyl-L-methionine levels and its application in enzyme studies.
A highly selective high-performance liquid chromatographic method for the quantification of human erythrocyte S-adenosyl-L-methionine levels is described. A strong cation-exchange sorbent with propylsulphonic acid functional groups was used to extract S-adenosyl-L-methionine and S-adenosylethionine (internal standard) from erythrocytes. Quantification of erythrocyte S-adenosyl-L-methionine levels was achieved by using reversed-phase high-performance liquid chromatography and ultraviolet detection at 254 nm. This method was adapted to measure methionine-adenosyltransferase activity in erythrocytes, which enables us to study the possible role of altered methylation in different diseases.
Lagendijk
J
J
Department of Chemical Pathology, Faculty of Medicine, University of Pretoria, South Africa.
Ubbink
J B
JB
Vermaak
W J
WJ
D002851:Chromatography, High Pressure Liquid; D004798:Enzymes / Q000097:blood; D004912:Erythrocytes / Q000737:chemistry* / Q000201:enzymology; D006801:Humans; D008716:Methionine Adenosyltransferase / Q000097:blood; D012436:S-Adenosylmethionine / Q000097:blood*; D013056:Spectrophotometry, Ultraviolet
D004798:Enzymes; D012436:S-Adenosylmethionine; D008716:Methionine Adenosyltransferase