[Introduction of statistical mutations into strictly defined areas of the genome using a modified oligonucleotide].
A method for inducing mutations in the short region of a gene is suggested. The method involves oligonucleotide modification by hydroxylamine derivative, in vitro enzymatic synthesis of double-stranded DNA using modified oligonucleotide as a primer and selection of mutant colonies using the starting unmodified oligonucleotide as a probe.
Smirnova
O Iu
OIu
Tat'kov
S I
SI
Il'ichev
A A
AA
Petrenko
V A
VA
D000595:Amino Acid Sequence; D001345:Autoradiography; D001483:Base Sequence; D004247:DNA / Q000096:biosynthesis; D004591:Electrophoresis, Polyacrylamide Gel; D000077190:Interferon alpha-2; D016898:Interferon-alpha / Q000235:genetics; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009841:Oligonucleotides / Q000737:chemistry*; D011994:Recombinant Proteins
D000077190:Interferon alpha-2; D016898:Interferon-alpha; D009841:Oligonucleotides; D011994:Recombinant Proteins; D004247:DNA
Vvedenie statisticheskikh mutatsiĭ v strogo opredelennyĭ raĭon genoma s pomoshch'iu modifitsirovannogo oligonukleotida.