title: A convenient method for regional monoamine oxidase-A determination by [14C]clorgyline autoradiography.
abstract: The availability of clorgyline for regional monoamine oxidase-A (MAO-A) determination was examined using [14C]clorgyline in rat. [14C]Clorgyline was synthesized by the methylation reaction of N-desmethyl-clorgyline and [14C]methyliodide in dimethylformamide with high radiochemical yield. The MAO-A distribution map by autoradiography correlated with that by histochemical technique and its quantity was consistent with the calculated MAO-A amount based on previous reports. The combination of labeled clorgyline and autoradiographic technique will promise the quantitative measurement of regional MAO-A distribution.
authors.affiliation: Department of Radiology, Research Institute for Brain and Blood Vessels-Akita, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Murakami
authors.forename: Y
authors.initials: Y
authors.lastname: Kondoh
authors.forename: Y
authors.initials: Y
authors.lastname: Weimin
authors.forename: S
authors.initials: S
authors.lastname: Mizusawa
authors.forename: H
authors.initials: H
authors.lastname: Nakamichi
authors.forename: K
authors.initials: K
authors.lastname: Takahashi
authors.forename: H
authors.initials: H
authors.lastname: Sasaki
authors.forename: H
authors.initials: H
authors.lastname: Iida
authors.forename: S
authors.initials: S
authors.lastname: Miura
authors.forename: I
authors.initials: I
authors.lastname: Kanno
mesh_terms: D000818:Animals; D001345:Autoradiography / Q000379:methods*; D001921:Brain / Q000201:enzymology; D002250:Carbon Radioisotopes; D003010:Clorgyline / Q000138:chemical synthesis; D008995:Monoamine Oxidase / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D014055:Tomography, Emission-Computed
chemical_list: D002250:Carbon Radioisotopes; D008995:Monoamine Oxidase; D003010:Clorgyline