Alteration of the c-fms gene in a blood sample from a Thorotrast individual.
We analyzed six different tissue DNA samples from a leukemic individual who received an injection of Thorotrast for alterations in proto-oncogene or tumor-suppressor gene structure. Our examination of the DNA indicated an alteration of the c-fms gene in the blood sample from this individual. This locus showed a deletion in which the 3' end of the deleted region maps between exons 11 and 12. In this particular case, the type of leukemia is unknown but myeloid leukemia is a neoplasm associated with individuals injected with Thorotrast. It is possible that the alteration in the c-fms gene of this individual is a consequence of the radiation exposure. No apparent alterations in the c-mos gene were observed in any of the tissues from the individual. This is in contrast to previous studies that described alterations in methylation patterns associated with the c-mos locus in radium-exposed individuals. A number of the individuals exposed to radium also had alterations of the retinoblastoma gene while no such alterations were observed in any tissue DNA samples from this Thorotrast case. It is possible that our inability to detect alterations of the c-mos and retinoblastoma gene may be attributable to the nature of alpha-emitting radionuclides or their distribution, or to the limited set of tissues available for analysis.
Collart
F R
FR
Biological and Medical Research Division, Argonne National Laboratory, IL 60439.
Horio
M
M
Schlenker
R A
RA
Kathren
R L
RL
Huberman
E
E
D000368:Aged; D003287:Contrast Media / Q000009:adverse effects*; D005260:Female; D016147:Genes, Tumor Suppressor / Q000528:radiation effects*; D016236:Genes, fms / Q000528:radiation effects*; D016258:Genes, mos / Q000528:radiation effects*; D006801:Humans; D007953:Leukemia, Radiation-Induced / Q000235:genetics*; D000090063:Proto-Oncogene Mas; D011831:Radiation Genetics; D013911:Thorium Dioxide / Q000009:adverse effects*; D013997:Time Factors
D003287:Contrast Media; C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D013911:Thorium Dioxide