A simple step to reduce background in E. coli transformations of blunt-ended plasmid ligation products.
Shepard
A R
AR
Department of Medicine, Mayo Clinic, Rochester, MN 55905.
Eberhardt
N L
NL
D003001:Cloning, Molecular / Q000379:methods*; D004926:Escherichia coli / Q000235:genetics*; D010957:Plasmids; D014169:Transformation, Bacterial