title: Constitutive transcription of the gene for metallothionein in a cadmium-resistant yeast.
abstract: A cadmium-resistant strain of Saccharomyces cerevisiae produces a cadmium metallothionein encoded by the CUP1 gene as does a copper-resistant strain. The mechanism of expression of the gene is inducible by copper ions in the copper-resistant strain. However, assays of CUP1-specific mRNA revealed that the transcription of the CUP1 gene in the cadmium-resistant strain is constitutive and the rate of transcription is further increased by exposure to cadmium or copper ions. This result was confirmed by the appearance of constitutive-expression segregants from diploid crosses between the cadmium-resistant strain and a strain with a reporter gene having the promoter of CUP1.
authors.affiliation: Department of Biology, Faculty of Science, Ehime University, Matsuyama, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Tohoyama
authors.forename: A
authors.initials: A
authors.lastname: Inagawa
authors.forename: H
authors.initials: H
authors.lastname: Koike
authors.forename: M
authors.initials: M
authors.lastname: Inouhe
authors.forename: M
authors.initials: M
authors.lastname: Joho
authors.forename: T
authors.initials: T
authors.lastname: Murayama
mesh_terms: D002104:Cadmium / Q000494:pharmacology*; D003300:Copper / Q000494:pharmacology; D004352:Drug Resistance, Microbial; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D008668:Metallothionein / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: C004203:cadmium-binding protein; D002104:Cadmium; D003300:Copper; D008668:Metallothionein