title: Membrane-bound choline acetyltransferase of the torpedo has characteristics of an integral membrane protein and can be solubilized by proteolysis.
abstract: Due to Triton X-114 fractionation of synaptosomes isolated from the electric organ of the fish Torpedo, the existence of a hydrophilic and an amphiphilic form of the enzyme choline-O-acetyltransferase (ChAT) was revealed. Amphiphilic ChAT which represents about 10% of total enzyme activity in synaptosomes, reached 40% of ChAT activity measured in preparations of synaptosomal plasma membranes (SPM) which were washed with solutions of increasing ionic strength. ChAT activity bound to washed SPM could be partially solubilized using proteinase K but not phospholipase C. No ChAT solubilization occurred by treating intact synaptosomes with proteinase K. Water/Triton X-114 partition coefficients of hydrophilic and amphiphilic ChAT were found to be 6.5 and 0.17, respectively. Sedimentation coefficients determined by centrifugation in linear density gradients of sucrose containing Triton X-100, were 4.2S and 4.4S for amphiphilic and hydrophilic ChAT, respectively. On the other hand, removal of Triton X-114 from the detergent phase containing amphiphilic ChAT activity led to enzyme aggregation. Finally, amphiphilic ChAT was slightly more acidic (pH 6.6) than was hydrophilic enzyme (6.8-7.0). We conclude that in Torpedo synaptosomes two forms of ChAT activity, a soluble and a membrane-bound form, are indeed present which differ in their hydrophobicity. The soluble form is hydrophilic. The membrane-bound form is amphiphilic and it aggregates upon removal of detergent. These are two characteristics of integral membrane proteins. Membrane-bound ChAT is most probably intracellularly oriented and not bound to membrane through a 'receptor' protein.
authors.affiliation: Department of Pharmacology, Centre Médical Universitaire, Geneva, Switzerland.
authors.forename: L
authors.initials: L
authors.lastname: Eder-Colli
authors.forename: P A
authors.initials: PA
authors.lastname: Briand
authors.forename: Y
authors.initials: Y
authors.lastname: Dunant
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism; D000818:Animals; D002499:Centrifugation, Density Gradient; D002795:Choline O-Acetyltransferase / Q000302:isolation & purification / Q000378:metabolism*; D003902:Detergents; D004557:Electric Organ / Q000201:enzymology*; D006735:Horseradish Peroxidase / Q000378:metabolism; D006867:Hydrolases; D007425:Intracellular Membranes / Q000201:enzymology*; D007527:Isoenzymes / Q000302:isolation & purification / Q000378:metabolism*; D007700:Kinetics; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008565:Membrane Proteins / Q000302:isolation & purification / Q000378:metabolism*; D017830:Octoxynol; D011092:Polyethylene Glycols; D013574:Synaptosomes / Q000201:enzymology*; D014101:Torpedo
chemical_list: D003902:Detergents; D007527:Isoenzymes; D008565:Membrane Proteins; D011092:Polyethylene Glycols; D017830:Octoxynol; C010615:Nonidet P-40; D007770:L-Lactate Dehydrogenase; D006735:Horseradish Peroxidase; D002795:Choline O-Acetyltransferase; D006867:Hydrolases; D000110:Acetylcholinesterase