Characterization and distribution of angiotensin II binding sites in fetal and neonatal astrocytes from different rat brain regions.
Although angiotensin II (Ang II) binding sites have been extensively investigated in brain, revealing the presence of both AT1 and AT2 subtypes in various areas, the question as to which cells express AT1 and AT2 sites is still open. We report here that primary cultures of astrocytes obtained from various brain regions of fetal (F17) and one-day-old rats express Ang II binding sites belonging only to the AT1 subtype. The binding sites have the same binding profile in all regions tested; however, much less binding was observed in membranes of astrocytes derived from cortical than from subcortical regions and almost none were found in neonatal cortex. In addition, the dispersion method used at the onset of culture affects the number of binding sites present at the end of the culture period.
Bottari
S P
SP
Cardiovascular Research, Ciba-Geigy, Basel, Switzerland.
Obermüller
N
N
Bogdal
Y
Y
Zahs
K R
KR
Deschepper
C F
CF
D000804:Angiotensin II / Q000037:antagonists & inhibitors / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism*; D001253:Astrocytes / Q000378:metabolism*; D001665:Binding Sites; D001667:Binding, Competitive; D001713:Biphenyl Compounds / Q000378:metabolism; D001921:Brain / Q000196:embryology / Q000378:metabolism*; D002478:Cells, Cultured; D005333:Fetus / Q000378:metabolism*; D007093:Imidazoles / Q000378:metabolism; D019808:Losartan; D009842:Oligopeptides / Q000494:pharmacology; D051381:Rats; D012504:Saralasin / Q000378:metabolism; D013777:Tetrazoles / Q000378:metabolism; D014018:Tissue Distribution
D001713:Biphenyl Compounds; D007093:Imidazoles; D009842:Oligopeptides; D013777:Tetrazoles; D000804:Angiotensin II; C060894:CGP 42112A; D012504:Saralasin; D019808:Losartan