title: [Identification of the gene for the immunodominant p35 protein from vaccinia virus].
abstract: A major immunodominant envelope protein p35 of vaccina virus was purified by means of extraction from virions with detergent NP-40. The protein was cleaved with CNBr, four homogenous peptides were isolated and their N-terminal amino acid sequences were determined. Computer search in a protein sequences data bank revealed that the immunodominant protein p35 of vaccinia virus is encoded by H3 gene in HindIII-H fragment of vaccinia virus genome.
authors.forename: V V
authors.initials: VV
authors.lastname: Zinov'ev
authors.forename: L G
authors.initials: LG
authors.lastname: Ovechkina
authors.forename: L V
authors.initials: LV
authors.lastname: Matskova
authors.forename: S M
authors.initials: SM
authors.lastname: Balakhnin
authors.forename: E G
authors.initials: EG
authors.lastname: Malygin
authors.forename: O Iu
authors.initials: OIu
authors.lastname: Chertov
authors.forename: I N
authors.initials: IN
authors.lastname: Telezhinskaia
authors.forename: E V
authors.initials: EV
authors.lastname: Zaĭtseva
authors.forename: T B
authors.initials: TB
authors.lastname: Golubeva
mesh_terms: D000595:Amino Acid Sequence; D015153:Blotting, Western; D002850:Chromatography, Gel; D003488:Cyanogen Bromide; D004591:Electrophoresis, Polyacrylamide Gel; D005814:Genes, Viral; D007106:Immune Sera; D016056:Immunodominant Epitopes / Q000235:genetics*; D008969:Molecular Sequence Data; D011863:Radioimmunoassay; D014616:Vaccinia virus / Q000235:genetics* / Q000276:immunology; D014759:Viral Envelope Proteins / Q000235:genetics*
chemical_list: D007106:Immune Sera; D016056:Immunodominant Epitopes; D014759:Viral Envelope Proteins; C072495:immunodominant protein p35, vaccinia virus; D003488:Cyanogen Bromide
vernacular_title: Identifikatsiia gena immunodominantnogo belka p35 virusa ospovaktsiny.