title: Effects of septal and/or raphe cell suspension grafts on hippocampal choline acetyltransferase activity, high affinity synaptosomal uptake of choline and serotonin, and behavior in rats with extensive septohippocampal lesions.
abstract: At 31 days of age, Long-Evans female rats sustained aspirative lesions of the septohippocampal pathways and, 14 days later, received intrahippocampal suspension grafts prepared from the region including the medial septum and the diagonal band of Broca (Group S, n = 11), from the region including the mesencephalic raphe (Group R, n = 11) or from both regions together (Group S+R, n = 11). Sham-operated (Group Sham, n = 9) and lesion-only (Group Les, n = 11) rats served as non-grafted controls. Seven Sham, 7 Les and 8 rats from each transplant group were tested for home cage activity (6 months after grafting) and radial maze performance (between 7.5 and 8.5 months post-grafting). One month after completion of behavioral testing, the dorsal hippocampi of these rats were prepared for measuring choline acetyltransferase (ChAT) activity and high affinity synaptosomal uptake of both [3H]choline and [3H]serotonin. The remaining rats were used for histological verifications on brain sections stained for acetylcholinesterase (AChE). The lesions increased locomotor activity, impaired radial maze learning and, in the dorsal hippocampus, reduced AChE positive staining, decreased ChAT activity (-73%) as well as high affinity uptake of both choline (-81%) and serotonin (-82%). Neither type of transplant produced any significant behavioral recovery. However, septal transplants increased hippocampal AChE positivity, restored ChAT activity and enhanced choline uptake to 116% and 70% of the values found in sham-operated rats, respectively; they had no significant effect on uptake of serotonin. Transplants from the raphe region had weak effects on hippocampal AChE positivity, increased both the ChAT activity and the choline uptake to 70% ad 38% of the sham-operated rats, respectively, and produced an (over)compensation of the serotonin uptake which reached 324% of the values found in sham-operated rats. The co-transplantation of both regions resulted in restoration of ChAT activity (113% of sham-operated rats values), choline uptake (83% of sham-operated rats) and serotonin uptake (129% of sham-operated rats). Our neurochemical data show that after extensive denervation of the hippocampus, intrahippocampal grafts of fetal neurons may foster a neurotransmitter-specific recovery which depends upon the anatomical origin of the grafted cells: a graft rich in serotonergic neurons overcompensates the serotonergic deficit, a graft rich in cholinergic neurons attenuates the cholinergic deficit, whereas a mixture of both types of grafts produces recovery from both types of deficits. Thereby, both the feasibility and the interest of the co-grafting technique are confirmed.(ABSTRACT TRUNCATED AT 400 WORDS)
authors.affiliation: Pharmakologisches Institut, Universität Freiburg, FRG.
authors.forename: J C
authors.initials: JC
authors.lastname: Cassel
authors.forename: B
authors.initials: B
authors.lastname: Neufang
authors.forename: C
authors.initials: C
authors.lastname: Kelche
authors.forename: F
authors.initials: F
authors.lastname: Aiple
authors.forename: B E
authors.initials: BE
authors.lastname: Will
authors.forename: G
authors.initials: G
authors.lastname: Hertting
authors.forename: R
authors.initials: R
authors.lastname: Jackisch
mesh_terms: D000818:Animals; D001522:Behavior, Animal / Q000502:physiology; D001667:Binding, Competitive; D002794:Choline / Q000378:metabolism; D002795:Choline O-Acetyltransferase / Q000378:metabolism*; D005260:Female; D006624:Hippocampus / Q000201:enzymology* / Q000502:physiology; D009474:Neurons / Q000637:transplantation*; D011903:Raphe Nuclei / Q000166:cytology*; D051381:Rats; D012688:Septum Pellucidum / Q000166:cytology* / Q000502:physiology; D012701:Serotonin / Q000378:metabolism; D013574:Synaptosomes / Q000378:metabolism
chemical_list: D012701:Serotonin; D002795:Choline O-Acetyltransferase; D002794:Choline