Effects of the prostanoid receptor antagonist, di-4-phloretin phosphate, upon human sperm motility.
The inhibitory effects of the prostanoid receptor antagonist, di-4-phloretin phosphate (DPP), upon washed human sperm motility were determined in vitro (up to 60 min of incubation). Concentrations tested were: 0.001, 0.01, 0.1, 0.25, 0.5, 2.5, 5.0 and 10.0 mM. All concentrations of DPP investigated caused cessation of sperm movement (percentage motility and average forward velocity) and the antimotility effect was essentially irreversible. The concentrations producing 50% inhibition were: percentage motility--1.31 +/- 0.56 mM (mean +/- SEM) and average forward velocity--1.95 +/- 0.68 mM. The mode of antimotility action appears to be multi-faceted. At high (10 mM) and intermediate (2.5 mM) concentrations, changes in fluidity of sperm plasmalemma (hypoosmotic swelling test) and loss of viability (nigrosin-eosin stain technique) constitute the primary means by which motility was disrupted. In contrast, these two parameters of motility remained unaltered at a lower concentration (0.25 mM) of DPP, and the mechanism precipitating the antimotility effect may involve mobilization of stored calcium ions and modulation of cyclic AMP levels.
Ratnasooriya
W D
WD
Department of Zoology, University of Colombo, Sri Lanka.
Premakumara
G A
GA
D000704:Analysis of Variance; D001219:Aspartate Aminotransferases / Q000032:analysis; D002110:Caffeine / Q000494:pharmacology; D002118:Calcium / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D009997:Osmotic Pressure / Q000187:drug effects; D011121:Polyphloretin Phosphate / Q000494:pharmacology*; D011448:Prostaglandin Antagonists / Q000494:pharmacology*; D013081:Sperm Motility / Q000187:drug effects*; D013094:Spermatozoa / Q000187:drug effects
D011448:Prostaglandin Antagonists; C013509:diphloretin phosphate; D002110:Caffeine; D011121:Polyphloretin Phosphate; D001219:Aspartate Aminotransferases; D002118:Calcium