A dominant selectable marker that is meiotically stable in Neurospora crassa: the amdS gene of Aspergillus nidulans.
When Neurospora crassa is transformed using a Neurospora gene as the selectable marker, the vegetatively stable transformants obtained cannot be used successfully in a cross because the selectable marker will be inactivated by the process of RIP (repeat-induced point mutation). Introduction of the acetamidase-encoding gene amdS of Aspergillus nidulans into N. crassa by transformation yielded transformants that would grow in minimal medium containing acetamide as a sole nitrogen source. In mitotically stable transformants containing a single copy of the amdS gene, the capacity to utilize acetamide as a sole nitrogen source was maintained in the progeny of a sexual cross. Therefore, the A. nidulans amdS gene is an appropriate dominant selectable marker for use in transformation analyses with N. crassa in which sexual crosses will be subsequently performed.
Yamashiro
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Department of Biological Sciences, Stanford University, CA 94305-5020.
Yarden
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Yanofsky
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C
D000581:Amidohydrolases / Q000235:genetics*; D001233:Aspergillus nidulans / Q000235:genetics*; D003433:Crosses, Genetic; D005799:Genes, Dominant; D005800:Genes, Fungal; D005819:Genetic Markers; D008540:Meiosis / Q000235:genetics*; D009492:Neurospora crassa / Q000166:cytology / Q000235:genetics*; D014170:Transformation, Genetic
D005819:Genetic Markers; D000581:Amidohydrolases; C021445:acetamidase