Spontaneous diabetes in bio-breeding rats: evidence for insulin dependent liver microsomal delta 9 stearic acid desaturation.
We studied liver microsomal delta 9 stearic acid desaturase activity and fatty acid composition of liver phospholipids and microsomal total lipids in the insulin-dependent spontaneously diabetic adult male Bio-Breeding rat. The diabetic Bio-Breeding rats (3 weeks of diabetes) were killed 48, 17 and 3 h after the last insulin injection (1.0 IU, 100 g body weight-1 day-1). Under these experimental conditions, delta 9 desaturase activity was defective during the normo- and hyper-glycaemic periods and restored during the hypoglycaemic period which followed the insulin injection to the diabetic rats. The fatty acid composition of diabetic rat liver phospholipids and microsomal total lipids were not consistent with delta 9 desaturase activity at the different periods of glycaemia and may be explained by factors other than disturbances of this desaturation.
Mimouni
V
V
Unité de Recherche de Nutrition Cellulaire et Métabolique, Université de Bourgogne, Faculté des Sciences Mirande, Dijon, France.
Narce
M
M
Poisson
J P
JP
D000818:Animals; D003920:Diabetes Mellitus / Q000235:genetics / Q000378:metabolism*; D004195:Disease Models, Animal; D005227:Fatty Acids / Q000378:metabolism*; D007328:Insulin / Q000494:pharmacology*; D050356:Lipid Metabolism; D008099:Liver / Q000378:metabolism; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism*; D010743:Phospholipids / Q000378:metabolism*; D051381:Rats; D011913:Rats, Inbred BB; D011919:Rats, Inbred Strains
D005227:Fatty Acids; D007328:Insulin; D010743:Phospholipids