Synthesis of the proline analogue [2,3-3H]azetidine-2-carboxylic acid. Uptake and incorporation in Arabidopsis thaliana and Escherichia coli.
Azetidine-2-carboxylic acid, the 4-membered ring noranalogue of proline, is regularly used in the study of proline metabolism as well as the study of protein conformation. We prepared D,L-[2,3-3H]azetidine-2-carboxylic acid with an optimized 10% yield from commercially available 4-amino-[2,3-3H]butyric acid. Purification was performed by fast-protein liquid chromatography. The biological activity was checked in both Arabidopsis thaliana and Escherichia coli. The obtained specific activity of 10 mCi/mmol was sufficient for most uptake and incorporation studies.
Verbruggen
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N
Laboratorium voor Genetica, Universiteit Gent, Belgium.
van Montagu
M
M
Messens
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E
D001383:Azetidinecarboxylic Acid / Q000138:chemical synthesis* / Q000378:metabolism*; D004926:Escherichia coli / Q000378:metabolism*; D007553:Isotope Labeling; D010944:Plants / Q000378:metabolism*; D014316:Tritium
D014316:Tritium; D001383:Azetidinecarboxylic Acid