Characterization of human thyrotropin receptor-related peptide-like immunoreactivity in peripheral blood of Graves' disease.
An antiserum raised against an alignment of amino acid-(32-56), termed TSHRP-1, in the extracellular domain of human thyrotropin (TSH) receptor was used to identify the TSH receptor-like substance in plasma of Graves' disease. The dilution curve of plasma TSHRP-1-like immunoreactivity was observed in a manner parallel to the standard synthetic peptide curve in radioimmunoassay, and its molecular weight estimated approximately 60 kDa. The amounts of TSHRP-1-like immunoreactivity were significantly higher in Graves' plasma than those in plasma of normal and hypothyroid patients due to Hashimoto's thyroiditis. The present results indicate that human peripheral blood possesses a soluble form of the extracellular domain of TSH receptor which may contribute to the pathophysiology of Graves' disease.
Murakami
M
M
First Department of Internal Medicine, Gunma University, School of Medicine, Maebashi, Japan.
Miyashita
K
K
Yamada
M
M
Iriuchijima
T
T
Mori
M
M
D015415:Biomarkers / Q000097:blood; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D006111:Graves Disease / Q000097:blood* / Q000276:immunology; D006801:Humans; D007106:Immune Sera; D007074:Immunoglobulin G; D007700:Kinetics; D008970:Molecular Weight; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology; D011863:Radioimmunoassay / Q000379:methods; D011989:Receptors, Thyrotropin / Q000032:analysis* / Q000302:isolation & purification / Q000378:metabolism
D015415:Biomarkers; D007106:Immune Sera; D007074:Immunoglobulin G; D010455:Peptides; D011989:Receptors, Thyrotropin