A useful modification of cDNA that enhances purification of recombinant protein.
With the availability of new and improved DNA vectors for producing large amounts of proteins by recombinant DNA technology, there is a growing need for efficient recovery of the desired protein, often from liters of culture medium. In this report, we describe a relatively simple modification of recombinant cDNA, that, in combination with immobilized metal affinity chromatography, is a rapid, inexpensive and highly effective method for enriching or purifying a desired protein.
Drake
L
L
Molecular Diagnostics, Inc., West Haven, CT 06516.
Barnett
T
T
D001483:Base Sequence; D002846:Chromatography, Affinity; D003062:Codon; D004274:DNA, Recombinant / Q000235:genetics*; D006639:Histidine / Q000235:genetics; D007098:Imino Acids; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000302:isolation & purification*; D012685:Sepharose / Q000031:analogs & derivatives
D003062:Codon; D004274:DNA, Recombinant; D007098:Imino Acids; D011994:Recombinant Proteins; C042660:zinc(II)-iminodiacetate agarose; D006639:Histidine; D012685:Sepharose