B-B10 (anti-CD25)-saporin immunotoxin--a possible tool in graft-versus-host disease treatment.
An immunotoxin containing the B-B10 MoAb, directed against the CD25 determinant, and the ribosome-inactivating protein saporin, inhibits 3H-TdR incorporation in phytohemagglutin, allogeneic-stimulated lymphocytes (primary and secondary mixed-lymphocyte reaction), and in an alloreactive T cell clone. A lower degree of inhibition was obtained with the B-B10 MoAb, which is known to inhibit IL-2 activity, as well as with the unconjugated compounds. These results suggest that the in vivo administration of the conjugate might be a more effective tool in the treatment of patients affected by graft-versus-host disease than B-B10 alone, by inducing an efficient killing of allogeneic-reacting T lymphocytes.
Tazzari
P L
PL
Istituto Nazionale per la Ricerca sul Cancro, Genoa, Italy.
Bolognesi
A
A
De Totero
D
D
Pileri
S
S
Conte
R
R
Wijdenes
J
J
Hervé
P
P
Soria
M
M
Stirpe
F
F
Gobbi
M
M
D000911:Antibodies, Monoclonal / Q000627:therapeutic use*; D001854:Bone Marrow Cells; D006412:Hematopoietic Stem Cells / Q000276:immunology; D006801:Humans; D007156:Immunologic Memory; D007165:Immunosuppression Therapy / Q000379:methods; D000922:Immunotoxins; D066298:In Vitro Techniques; D008213:Lymphocyte Activation; D009699:N-Glycosyl Hydrolases; D010940:Plant Proteins / Q000008:administration & dosage*; D015375:Receptors, Interleukin-2 / Q000276:immunology*; D054789:Ribosome Inactivating Proteins, Type 1; D000076985:Saporins; D013601:T-Lymphocytes / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000922:Immunotoxins; D010940:Plant Proteins; D015375:Receptors, Interleukin-2; D054789:Ribosome Inactivating Proteins, Type 1; D009699:N-Glycosyl Hydrolases; D000076985:Saporins