Laminin--developmental expression and role in axonal outgrowth in the peripheral nervous system of the chick.
The possible role of laminin on axon outgrowth and guidance in vivo was examined by: (1) determining its developmental expression, and relationship to outgrowth of sensory, motor and sympathetic axons in the chick embryo; and (2) evaluating the changes in the pattern of sympathetic preganglionic projections subsequent to injections of laminin, antilaminin and other laminin function blockers (JG22, INO) into their pathways during axon outgrowth. Double immunofluorescent staining for laminin and neurofilaments in peripheral nerves prior to and during initial outgrowth showed no obvious relationship between laminin and potential nerve pathways. Even though weak laminin immunostaining is apparent throughout the mesenchyme through which axons grow, the most prominent laminin immunostaining is on basement membranes of the neural tube, notochord and dermamyotome. However, as peripheral nerves mature, laminin becomes localized to nerve fascicles throughout the peripheral nervous system, beginning with the dorsal and ventral roots, and progressing later to more distal spinal nerves. Microinjections of antilaminin, JG22 (a monoclonal antibody against laminin/fibronectin receptors) and INO (a monoclonal antibody against a laminin-heparan sulfate proteoglycan complex) into the pathway of sympathetic preganglionic axons prior to and during outgrowth had no effect on the spatio-temporal patterns of sympathetic preganglionic projections. An alternate laminin-rich pathway produced by injecting laminin into the region of the sympathetic trunk immediately adjacent but caudal to the T1 spinal level also did not alter the projection of T1 preganglionic axons. These results suggest that laminin may not be crucial to the initial of peripheral axons. The localization of laminin in nerve fascicles in later stages of development suggests instead that laminin may be important in the maintenance of these structures.
Yip
J W
JW
Department of Physiology, School of Medicine, University of Pittsburgh, PA 15261.
Yip
Y P
YP
D000818:Animals; D001339:Autonomic Fibers, Preganglionic / Q000502:physiology*; D001369:Axons / Q000502:physiology*; D002478:Cells, Cultured; D002642:Chick Embryo; D005455:Fluorescent Antibody Technique; D007797:Laminin / Q000032:analysis / Q000502:physiology*; D008845:Microinjections; D009046:Motor Neurons / Q000187:drug effects; D009475:Neurons, Afferent / Q000187:drug effects; D010525:Peripheral Nerves / Q000737:chemistry / Q000196:embryology*; D013116:Spinal Cord / Q000166:cytology / Q000187:drug effects / Q000196:embryology; D013564:Sympathetic Nervous System / Q000737:chemistry / Q000196:embryology*
D007797:Laminin