Cloning and sequencing of hepatitis B virus pre-S and S gene regions.
The polymerase chain reaction (PCR) was used to amplify the pre-S1, pre-S2 and S gene regions of hepatitis B virus (HBV). Sera from three different patients were used as the source of HBV DNA. The resulting 1.2-kb amplification product was cloned into the plasmid pIBI30. Restriction enzyme analysis revealed that there are two BamHI sites located about 300 bp apart within the S gene. DNA sequencing revealed a greatest homology to the HBVadw subtype.
Chirara
M M
MM
Department of Biochemistry, University of Zimbabwe, Mount Pleasant, Harare.
Chetsanga
C J
CJ
D001483:Base Sequence; D003001:Cloning, Molecular; D006514:Hepatitis B Surface Antigens / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D011498:Protein Precursors / Q000235:genetics*
D006514:Hepatitis B Surface Antigens; D011498:Protein Precursors; C050806:presurface protein 1, hepatitis B surface antigen; C050807:presurface protein 2, hepatitis B surface antigen