Successful graft of HTLV-I-transformed human T-cells (MT-2) in severe combined immunodeficiency mice treated with anti-asialo GM-1 antibody.
To develop an experimental model of adult T-cell leukemia/lymphoma in small animals, severe combined immunodeficiency (SCID) mice treated with anti-asialo GM-1 antibody were inoculated with MT-2 cells, a cell line transformed by the human T-cell leukemia virus (HTLV-I). Three mice injected with 4 x 10(7) cells subcutaneously or intramuscularly developed tumors at or near inoculation sites. Immunofluorescent antibody (IFA) staining for HTLV-I structural protein, p19, revealed the specific antigen in the cytoplasm of most cells from tumors and the DNA signals of HTLV-I proviral DNA were also positive in cellular DNA by polymerase chain reaction assay with HTLV-I tax gene primers, SK43/SK44. The MT-2 cells did not invade in mouse organs.
Ishihara
S
S
Second Department of Medicine, Miyazaki Medical College, Japan.
Tachibana
N
N
Okayama
A
A
Murai
K
K
Tsuda
K
K
Mueller
N
N
D000818:Animals; D000906:Antibodies / Q000008:administration & dosage*; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral; D004273:DNA, Neoplasm / Q000235:genetics / Q000302:isolation & purification; D005455:Fluorescent Antibody Technique; D005677:G(M1) Ganglioside / Q000276:immunology*; D015368:Human T-lymphotropic virus 1 / Q000235:genetics*; D006801:Humans; D015458:Leukemia, T-Cell / Q000473:pathology*; D016399:Lymphoma, T-Cell / Q000473:pathology*; D051379:Mice; D016513:Mice, SCID; D009368:Neoplasm Transplantation; D016133:Polymerase Chain Reaction; D013601:T-Lymphocytes / Q000637:transplantation*; D014183:Transplantation, Heterologous
D000906:Antibodies; D004273:DNA, Neoplasm; D005677:G(M1) Ganglioside; C018835:asialo GM1 ganglioside