title: Promotion of beta-structure by interaction of diabetes associated polypeptide (amylin) with phosphatidylcholine.
abstract: The interaction of the diabetes associated polypeptide (amylin) with dimyristoylphosphatidylcholine (DMPC) was assessed by measurements of turbidity (absorbance at 400 nm) and secondary structure by CD spectroscopy. In trifluoroethanol, human amylin adopts a highly alpha-helical conformation while the rat peptide is less structured. In water, the rat peptide is largely disordered and the human peptide exhibits a combination of alpha- and beta-structures. Mixtures of DMPC and the rat peptide have no effect on either the turbidity of the DMPC or the CD spectrum of the peptide. By contrast, mixtures of the human peptide with DMPC form relatively clear mixtures similar to those observed with amphipathic alpha-helical peptides, but the structure adopted, based on the CD spectrum, is largely beta. These data demonstrate that fundamental differences in the structures adopted by amylins from human and rat species exist in mixtures with DMPC and suggest that these differences may be related to the formation of amyloid fibrils in the human amylin peptide which are not observed in the rat peptide.
authors.affiliation: Marion Merrell Dow Research Institute, Cincinnati, OH 45215.
authors.forename: L R
authors.initials: LR
authors.lastname: McLean
authors.forename: A
authors.initials: A
authors.lastname: Balasubramaniam
mesh_terms: D000595:Amino Acid Sequence; D000682:Amyloid / Q000737:chemistry*; D000818:Animals; D002942:Circular Dichroism; D004134:Dimyristoylphosphatidylcholine / Q000494:pharmacology; D006801:Humans; D058228:Islet Amyloid Polypeptide; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009478:Neuropeptide Y / Q000737:chemistry; D010713:Phosphatidylcholines / Q000494:pharmacology*; D011487:Protein Conformation / Q000187:drug effects; D051381:Rats
chemical_list: D000682:Amyloid; D058228:Islet Amyloid Polypeptide; D009478:Neuropeptide Y; D010713:Phosphatidylcholines; D004134:Dimyristoylphosphatidylcholine