[Production of prostacyclin in human umbilical cord blood vessels. Effect of natural and synthetic estrogen].
This investigation was conducted to evaluate the effects of 17 beta-estradiol and 17 alpha-ethinylestradiol on prostacyclin PGI2-production in homogenates of human umbilical cord arteries and veins in the presence of exogenous arachidonic acid. The incubation of 17 beta-estradiol at 2 different concentrations (10(-8) and 10(-6) M) in the presence of arachidonic acid for various times (5 min up to 24 h) effected an increase in the production of PGI2 (measured as 6-keto-prostaglandin F1 alpha) of 36-47% in the artery and 16-21% in the vein over basal values (100%). Under the same experimental conditions with 17 alpha-ethinylestradiol we observed an increases of 30-40% in the artery and 1-18% in the vein compared with basal values. For both estrogens this was not significantly different compared with untreated samples. In our in vitro system, natural and synthetic estrogens had no clear effect on PGI2 production in homogenates of human umbilical vessels.
Mück
A O
AO
Sektion für klinische Pharmakologie in Gynäkologie und Geburtshilfe, Universitäts-Frauenklinik Tübingen.
Guo
Y
Y
Seeger
H
H
Korte
K
K
Lippert
T H
TH
D015121:6-Ketoprostaglandin F1 alpha / Q000378:metabolism; D046508:Culture Techniques; D011464:Epoprostenol / Q000096:biosynthesis*; D004958:Estradiol / Q000494:pharmacology*; D004997:Ethinyl Estradiol / Q000494:pharmacology*; D005260:Female; D006801:Humans; D007231:Infant, Newborn; D008431:Maternal-Fetal Exchange / Q000187:drug effects*; D011247:Pregnancy; D014469:Umbilical Arteries / Q000187:drug effects*; D014471:Umbilical Veins / Q000187:drug effects*
D004997:Ethinyl Estradiol; D004958:Estradiol; D015121:6-Ketoprostaglandin F1 alpha; D011464:Epoprostenol
Produktion von Prostacyclin in menschlichen Nabelschnur-Gefässen. Einfluss von natürlichem und synthetischem Estrogen.