title: Sec23p and a novel 105-kDa protein function as a multimeric complex to promote vesicle budding and protein transport from the endoplasmic reticulum.
abstract: A cell-free protein transport reaction has been used to monitor the purification of a functional form of the Sec23 protein, a SEC gene product required for the formation or stability of protein transport vesicles that bud from the endoplasmic reticulum (ER). Previously, we reported that Sec23p is an 84-kDa peripheral membrane protein that is released from a sedimentable fraction by vigorous mechanical agitation of yeast cells and is required for ER to Golgi transport assayed in vitro. We have purified soluble Sec23p by complementation of an in vitro ER to Golgi transport reaction reconstituted with components from sec23 mutant cells. Sec23p overproduced in yeast exists in two forms: a monomeric species and a species that behaves as a 250- to 300-kDa complex that contains Sec23p and a distinct 105-kDa polypeptide (p105). Sec23p purified from cells containing one SEC23 gene exists solely in the large multimeric form. A stable association between Sec23p and p105 is confirmed by cofractionation of the two proteins throughout the purification. p105 is a novel yeast protein involved in ER to Golgi transport. Like Sec23p, it is required for vesicle budding from the ER because p105 antiserum completely inhibits transport vesicle formation in vitro.
authors.affiliation: Division of Biochemistry and Molecular Biology, University of California, Berkeley 94720.
authors.forename: L
authors.initials: L
authors.lastname: Hicke
authors.forename: T
authors.initials: T
authors.lastname: Yoshihisa
authors.forename: R
authors.initials: R
authors.lastname: Schekman
mesh_terms: D000818:Animals; D000908:Antibodies, Fungal; D001692:Biological Transport / Q000502:physiology; D001704:Biopolymers; D004721:Endoplasmic Reticulum / Q000378:metabolism* / Q000648:ultrastructure; D005656:Fungal Proteins / Q000302:isolation & purification* / Q000378:metabolism; D006056:Golgi Apparatus / Q000378:metabolism*; D011817:Rabbits; D012441:Saccharomyces cerevisiae / Q000378:metabolism* / Q000648:ultrastructure
chemical_list: D000908:Antibodies, Fungal; D001704:Biopolymers; D005656:Fungal Proteins