title: [Nucleotide sequence of the pNB2 plasmid from thermophilic bacteria Clostridium thermosaccharolyticum].
abstract: The complete nucleotide sequence of pNB2, a 1.9-kilobases cryptic plasmid from thermophilic Clostridium thermosaccharolyticum has been determined. The plasmid consists of 1882 base pairs and has a G+C composition of 27.2%. The sequence contains three open reading frames capable of coding for polypeptides two of which were identified in maxicell Escherichia coli extracts. Our future studies are directed toward a construction of pNB2-derivatives as vectors for Clostridia.
authors.forename: N G
authors.initials: NG
authors.lastname: Belogurova
authors.forename: E P
authors.initials: EP
authors.lastname: Del'ver
authors.forename: S V
authors.initials: SV
authors.lastname: Kaliuzhnyĭ
authors.forename: S D
authors.initials: SD
authors.lastname: Varfolomeev
authors.forename: O V
authors.initials: OV
authors.lastname: Rodzevich
authors.forename: A A
authors.initials: AA
authors.lastname: Belogurov
mesh_terms: D000595:Amino Acid Sequence; D001345:Autoradiography; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D003013:Clostridium / Q000235:genetics* / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010957:Plasmids; D015183:Restriction Mapping
chemical_list: D001426:Bacterial Proteins
vernacular_title: Nukleotidnaia posledovatel'nost' plazmidy pNB2 iz termofil'nykh bakteriĭ Clostridium thermosaccharolyticum.