[Nucleotide sequence of the pNB2 plasmid from thermophilic bacteria Clostridium thermosaccharolyticum].
The complete nucleotide sequence of pNB2, a 1.9-kilobases cryptic plasmid from thermophilic Clostridium thermosaccharolyticum has been determined. The plasmid consists of 1882 base pairs and has a G+C composition of 27.2%. The sequence contains three open reading frames capable of coding for polypeptides two of which were identified in maxicell Escherichia coli extracts. Our future studies are directed toward a construction of pNB2-derivatives as vectors for Clostridia.
Belogurova
N G
NG
Del'ver
E P
EP
Kaliuzhnyĭ
S V
SV
Varfolomeev
S D
SD
Rodzevich
O V
OV
Belogurov
A A
AA
D000595:Amino Acid Sequence; D001345:Autoradiography; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D003013:Clostridium / Q000235:genetics* / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010957:Plasmids; D015183:Restriction Mapping
D001426:Bacterial Proteins
Nukleotidnaia posledovatel'nost' plazmidy pNB2 iz termofil'nykh bakteriĭ Clostridium thermosaccharolyticum.