Generation of analytic plasma lipoprotein profiles using two prepacked superose 6B columns.
A simple, low-priced chromatographic system to generate plasma lipoprotein profiles from total human plasma was tested with plasma from normolipidemic subjects and patients with heterozygous familial hypercholesterolemia, hyper-alpha-lipoproteinemia, lipoprotein lipase deficiency, familial dysbetalipoproteinemia and familial lecithin-cholesterol-acyl-transferase deficiency. The system appears to be a good alternative to more expensive high-pressure liquid chromatography systems, notably in lipoprotein laboratories already provided with equipment for column chromatography. If a microplate photometer and a computer is available in the laboratory, the measurement of various lipids in 70-80 eluant fractions from the columns can be simplified.
Gerdes
L U
LU
Department of Internal Medicine and Cardiology A, Aarhus County Hospital, University of Aarhus, Denmark.
Gerdes
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C
Klausen
I C
IC
Faergeman
O
O
D000328:Adult; D002784:Cholesterol / Q000097:blood; D002853:Chromatography, Liquid / Q000379:methods; D005260:Female; D006801:Humans; D006949:Hyperlipidemias / Q000097:blood; D008074:Lipoproteins / Q000097:blood*; D008075:Lipoproteins, HDL / Q000097:blood; D008077:Lipoproteins, LDL / Q000097:blood; D008079:Lipoproteins, VLDL / Q000097:blood; D008297:Male; D008875:Middle Aged; D015203:Reproducibility of Results; D012685:Sepharose; D014280:Triglycerides / Q000097:blood
D008074:Lipoproteins; D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL; D008079:Lipoproteins, VLDL; D014280:Triglycerides; C043127:Superose 6b; D012685:Sepharose; D002784:Cholesterol