What is tityustoxin?
Tityus serrulatus scorpion venom was fractionated by gel filtration affording two heterogeneous toxic fractions, T1 and T2; the latter was further fractionated by ion-exchange chromatography. The fraction of T2 eluted with 0.15 M ammonium acetate buffer, originally named 'tityustoxin', was shown to be a pool of several proteins. One of them, TsTX, as well as T1, was also named 'tityustoxin'. The major and perhaps most potent toxin of the venom, gamma-toxin, was eluted with 0.30 M buffer as a highly purified protein and shown to be different from TsTX. gamma-Toxin is contained in both T1 and T2.
Arantes
E C
EC
Departamento de Física e Química, Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, Brazil.
Sampaio
S V
SV
Vieira
C A
CA
Giglio
J R
JR
D002850:Chromatography, Gel; D009498:Neurotoxins / Q000302:isolation & purification*; D012604:Scorpion Venoms / Q000302:isolation & purification*
D009498:Neurotoxins; D012604:Scorpion Venoms; C003609:tityustoxin