Influence of male sexual rest and oocyte aging on parthenogenesis frequency in mice: cytogenetic analysis after in vitro fertilization.
This study was conducted to evaluate the influence of male sexual rest and oocyte aging on fertilization rate and parthenogenesis frequency after in vitro fertilization of mouse oocytes. We used a comparison between cleavage rates and fertilization rates according to chromosomal analysis of oocytes to estimate the parthenogenesis frequency. Fertilization rate was not impaired by male sexual rest. Parthenogenesis frequency was increased by male sexual rest. This effect was enhanced by a concomitant moderate oocyte aging. It is concluded that cleavage rate could not be considered as a reliable test of fertilization after attempted in vitro fertilization in such conditions.
Bergere
M
M
Laboratoire de Biologie de la Reproduction et du Développement Histologie Embryologie Cytogénétique, Centre Hospitalo, Universitaire de Bicêtre, France.
Selva
J
J
Dulioust
E
E
Rince
P
P
Auroux
M
M
D000367:Age Factors; D000818:Animals; D002455:Cell Division; D005260:Female; D005306:Fertilization / Q000502:physiology; D005307:Fertilization in Vitro; D008297:Male; D051379:Mice; D009865:Oocytes / Q000502:physiology*; D010312:Parthenogenesis; D012746:Sexual Abstinence