Nuclear proteins from Capan-2 cell line form specific complexes with the 17-1A antigen gene promoter.
To determine the location of sites important for the function of the 17-1A antigen gene promoter and to characterize the protein factors binding to these sites, fragments of the promoter region were analysed by gel retardation assay with nuclear extracts from Capan 2 cell line. At least two separate regions, which specifically bind nuclear proteins, were identified within the 5'flanking region of the 17-1A antigen gene. These regions have been located between nucleotides -877 to -518 (distal region) and -193 to +3 (proximal region) and presumably participate in regulation of expression of the 17-1A antigen gene.
Siemieniako
B
B
Institute of Human Genetics, Polish Academy of Sciences, Poznań.
Wiland
E
E
D000230:Adenocarcinoma; D000951:Antigens, Neoplasm / Q000235:genetics*; D001483:Base Sequence; D004268:DNA-Binding Proteins / Q000302:isolation & purification / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D005810:Multigene Family; D009687:Nuclear Proteins / Q000302:isolation & purification / Q000378:metabolism*; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis / Q000378:metabolism*; D010190:Pancreatic Neoplasms; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D015183:Restriction Mapping; D013379:Substrate Specificity; D016385:TATA Box
D000951:Antigens, Neoplasm; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D009838:Oligodeoxyribonucleotides; C050928:carcinoma-associated antigen 17-1A