title: An extracellular enzyme from Fusarium solani f. sp. phaseoli which catalyses hydration of the isoflavonoid phytoalexin, phaseollidin.
abstract: Among the antimicrobial phytoalexins produced by Phaseolus vulgaris (French bean) are the prenylated isoflavonoids kievitone and phaseollidin. Two enzyme activities, kievitone hydratase and phaseollidin hydratase, occur in culture filtrates of the bean pathogen, Fusarium solani f. sp. phaseoli, and catalyse similar hydration reactions on the dimethylallyl moieties of the phytoalexins. The enzymes nearly co-purified during hydroxyapatite chromatography followed by preparative native gel electrophoresis. Eluates from successive slices taken from the native gel were assayed for both activities. Although they were not completely separated in the native gel, the activity profiles indicated that the two activities were distinct. The Km of phaseollidin hydratase for phaseollidin was approximately 7 microM.
authors.affiliation: Department of Plant Pathology, University of Kentucky, Lexington 40546-0091.
authors.forename: C S
authors.initials: CS
authors.lastname: Turbek
authors.forename: D A
authors.initials: DA
authors.lastname: Smith
authors.forename: C L
authors.initials: CL
authors.lastname: Schardl
mesh_terms: D002384:Catalysis; D005419:Flavonoids / Q000378:metabolism*; D005656:Fungal Proteins / Q000494:pharmacology*; D005670:Fusarium / Q000201:enzymology*; D006836:Hydro-Lyases / Q000187:drug effects / Q000378:metabolism*; D010936:Plant Extracts / Q000378:metabolism*; D012717:Sesquiterpenes; D013729:Terpenes; D000094162:Phytoalexins
chemical_list: D005419:Flavonoids; D005656:Fungal Proteins; D010936:Plant Extracts; D012717:Sesquiterpenes; D013729:Terpenes; D006836:Hydro-Lyases; D000094162:Phytoalexins