Immunisation of cattle against theileriosis in Nakuru District of Kenya by infection and treatment and the introduction of unconventional tick control.
One hundred and one cross European-Boran cattle (50 cows and 51 calves), on a farm in Nakuru District, Kenya, were immunised against theileriosis using Theileria parva lawrencei and Theileria parva parva stocks from another district of Kenya. The stabilates used were T.p.lawrencei (Mara III) used at 10(-1.7) dilution and T.p.parva (Kilae) used at 10(-1.0) dilution. The stabilates were combined and inoculated simultaneously with a short-acting formulation of oxytetracycline hydrochloride given intramuscularly at 10 mg kg-1 body weight and was repeated on Day 4 after inoculation of the stabilate. Most of the theileriosis challenge on the farm was thought to be derived directly from the African buffalo (Syncerus caffer). Nine percent of the cattle had significant indirect fluorescent antibody (IFA) titres before the immunisation and 99% after immunisation. The immunised cattle were exposed to tick-borne disease challenge on the farm by withdrawal of acaricide cover. The immunised cattle were divided into five groups plus two susceptible control cows and two calves for each group. Cattle in four of the groups had acaricidal ear tags, each group having a different type, applied to both ears and the fifth group remained untagged. The animals remained without conventional acaricide application for 134 days. Ten out of 20 (50%) non-immunised control cattle became T.p.lawrencei reactors which only one out of 97 (1%) of the immunised cattle reacted. A frequent complication noted was mild infections due to unidentified Theileria sp. which required expert differentiation from T.parva infections. An additional group of ten steers whose tick load was removed by hand at weekly intervals was introduced 79 days after exposure; these had no tick control and four became T.p.lawrencei reactors. Of 12 calves born during the exposure period and without tick control, four became theilerial reactors and one died. The application of acaricidal tags however, reduced tick infestation levels considerably compared with untagged controls but did not prevent transmission of theileriosis with the possible exception of tags on Group 4. A number of transient low grade fevers were noted and attributed to Theileria sp., Ehrlichia bovis, Ehrlichia (Cytoecetes) ondiri and Borrelia theileri infections, none of which were fatal. One immunised animal died of acute dual infection of Babesia bigemina and Borrelia theileri after acaricide control by spraying was re-introduced but no Anaplasma infections were detected. An analysis of the economic effects of immunisation was made.
Young
A S
AS
Protozoology Division, National Veterinary Research Centre, Muguga, Kenya.
Mutugi
J J
JJ
Kariuki
D P
DP
Lampard
D
D
Maritim
A C
AC
Ngumi
P N
PN
Linyonyi
A
A
Leitch
B L
BL
Ndungu
S G
SG
Lesan
A C
AC
D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D002417:Cattle; D005260:Female; D007114:Immunization / Q000662:veterinary*; D007306:Insecticides / Q000008:administration & dosage; D008297:Male; D010118:Oxytetracycline / Q000627:therapeutic use; D016797:Theileria parva / Q000276:immunology*; D013801:Theileriasis / Q000188:drug therapy / Q000191:economics / Q000276:immunology / Q000517:prevention & control*; D013983:Tick Control / Q000191:economics; D013984:Tick Infestations / Q000517:prevention & control / Q000662:veterinary*; D013987:Ticks
D000913:Antibodies, Protozoan; D007306:Insecticides; D010118:Oxytetracycline