title: Three-dimensional crystal structure of recombinant murine interferon-beta.
abstract: The crystal structure of recombinant murine interferon-beta (IFN-beta) has been solved by the multiple isomorphous replacement method and refined to an R-factor of 20.5% against 2.6 A X-ray diffraction data. The structure shows a variant of the alpha-helix bundle with a new chain-folding topology, which seems to represent a basic structural framework of all the IFN-alpha and IFN-beta molecules belonging to the type I family. Functionally important segments of the polypeptide chain, as implied through numerous gene manipulation studies carried out so far, are spatially clustered indicating the binding site(s) to the receptor(s). Comparison of the present structure with those of other alpha-helical cytokine proteins, including porcine growth hormone, interleukin 2 and interferon gamma, indicated either a topological similarity in chain folding or a similar spatial arrangement of the alpha-helices.
authors.affiliation: Faculty of Engineering, Nagaoka University of Technology, Niigata, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Senda
authors.forename: T
authors.initials: T
authors.lastname: Shimazu
authors.forename: S
authors.initials: S
authors.lastname: Matsuda
authors.forename: G
authors.initials: G
authors.lastname: Kawano
authors.forename: H
authors.initials: H
authors.lastname: Shimizu
authors.forename: K T
authors.initials: KT
authors.lastname: Nakamura
authors.forename: Y
authors.initials: Y
authors.lastname: Mitsui
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D005075:Biological Evolution; D003461:Crystallography; D016207:Cytokines / Q000737:chemistry; D016899:Interferon-beta / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000737:chemistry; D012689:Sequence Homology, Nucleic Acid
chemical_list: D016207:Cytokines; D011994:Recombinant Proteins; D016899:Interferon-beta