A cocaine analog and a GBR analog label the same protein in rat striatal membranes.
Because some evidence suggests that cocaine and GBR12935 bind to different sites, we utilized photoaffinity probes from both classes of compounds to see if they label the same protein. [125I]RTI-82 a cocaine analog, and [125I]DEEP, a GBR analog, labeled protein(s) showing the same molecular weight, a similar pharmacological profile and a similar sensitivity to neuraminidase.
Patel
A
A
Neuroscience Branch, Addiction Research Center, National Institute on Drug Abuse, Baltimore, MD 21224.
Boja
J W
JW
Lever
J
J
Lew
R
R
Simantov
R
R
Carroll
F I
FI
Lewin
A H
AH
Philip
A
A
Gao
Y
Y
Kuhar
M J
MJ
D000818:Animals; D001386:Azides / Q000378:metabolism / Q000494:pharmacology*; D001665:Binding Sites; D002352:Carrier Proteins / Q000302:isolation & purification / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D003042:Cocaine / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D003342:Corpus Striatum / Q000378:metabolism*; D004298:Dopamine / Q000378:metabolism; D050483:Dopamine Plasma Membrane Transport Proteins; D004591:Electrophoresis, Polyacrylamide Gel; D007457:Iodine Radioisotopes; D008024:Ligands; D008562:Membrane Glycoproteins / Q000302:isolation & purification / Q000378:metabolism; D026901:Membrane Transport Proteins; D009419:Nerve Tissue Proteins; D010879:Piperazines / Q000378:metabolism*; D051381:Rats
D001386:Azides; D002352:Carrier Proteins; D050483:Dopamine Plasma Membrane Transport Proteins; D007457:Iodine Radioisotopes; D008024:Ligands; D008562:Membrane Glycoproteins; D026901:Membrane Transport Proteins; D009419:Nerve Tissue Proteins; D010879:Piperazines; C076379:RTI 82; C044630:1-(2 (diphenylmethoxy)ethyl)-4-(3-phenylpropyl)piperazine; D003042:Cocaine; D004298:Dopamine