title: Quantification of erythrocyte S-adenosyl-L-methionine levels and its application in enzyme studies.
abstract: A highly selective high-performance liquid chromatographic method for the quantification of human erythrocyte S-adenosyl-L-methionine levels is described. A strong cation-exchange sorbent with propylsulphonic acid functional groups was used to extract S-adenosyl-L-methionine and S-adenosylethionine (internal standard) from erythrocytes. Quantification of erythrocyte S-adenosyl-L-methionine levels was achieved by using reversed-phase high-performance liquid chromatography and ultraviolet detection at 254 nm. This method was adapted to measure methionine-adenosyltransferase activity in erythrocytes, which enables us to study the possible role of altered methylation in different diseases.
authors.affiliation: Department of Chemical Pathology, Faculty of Medicine, University of Pretoria, South Africa.
authors.forename: J
authors.initials: J
authors.lastname: Lagendijk
authors.forename: J B
authors.initials: JB
authors.lastname: Ubbink
authors.forename: W J
authors.initials: WJ
authors.lastname: Vermaak
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004798:Enzymes / Q000097:blood; D004912:Erythrocytes / Q000737:chemistry* / Q000201:enzymology; D006801:Humans; D008716:Methionine Adenosyltransferase / Q000097:blood; D012436:S-Adenosylmethionine / Q000097:blood*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D004798:Enzymes; D012436:S-Adenosylmethionine; D008716:Methionine Adenosyltransferase