Bacterial uptake of 14C-chlorhexidine diacetate and 14C-benzyl alcohol and the influence of phenoxyethanol and azolectin: studies with gram-negative bacteria.
The uptake of 14C-chlorhexidine (14C-CHA) by Pseudomonas aeruginosa and smooth, rough and deep rough strains of Escherichia coli was very rapid with maximum uptake occurring within 20 s. Despite the rapid binding, the lethal action of CHA, although concentration-dependent, is comparatively slow and occurs in minutes rather than seconds. This indicates that the initial rapid binding is followed by a second slower action, responsible for the lethal effects of CHA. The lethal action could be accelerated, particularly at modest concentrations of CHA, by the simultaneous presence of phenoxyethanol (POE) or benzyl alcohol (BZA), although the magnitude of the effect was small. Both alcohols had little effect on the binding of 14C-CHA, which does not explain the enhanced bactericidal action of CHA. Uptake of 14C-benzyl alcohol (14C-BZA) by the same strains showed very different patterns with slower and time-related binding. CHA had a marked effect on BZA absorption but no direct link was established between binding patterns and cell death. The CHA neutraliser, azolectin, removed bound CHA (in the presence or absence of POE) very efficiently even at contact times of only 20 s.
Fitzgerald
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KA
Welsh School of Pharmacy, University of Wales College of Cardiff, Great Britain.
Davies
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A
Russell
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AD
D000891:Anti-Infective Agents, Local / Q000494:pharmacology*; D019905:Benzyl Alcohol; D001592:Benzyl Alcohols / Q000378:metabolism*; D002710:Chlorhexidine / Q000378:metabolism*; D004926:Escherichia coli / Q000187:drug effects / Q000378:metabolism*; D005026:Ethylene Glycols / Q000494:pharmacology*; D006090:Gram-Negative Bacteria / Q000187:drug effects / Q000378:metabolism; D010713:Phosphatidylcholines; D010743:Phospholipids / Q000494:pharmacology*; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000378:metabolism*; D013104:Spheroplasts / Q000187:drug effects / Q000378:metabolism
D000891:Anti-Infective Agents, Local; D001592:Benzyl Alcohols; D005026:Ethylene Glycols; D010713:Phosphatidylcholines; D010743:Phospholipids; C024990:asolectin; C005398:phenoxyethanol; D019905:Benzyl Alcohol; D002710:Chlorhexidine