A flow-cytometric method to study DNA fragmentation in lymphocytes.
A method to measure DNA fragmentation cell by cell in a cell population was implemented based on acridine orange procedure to determine DNA content of single cells by flow cytometry. Using this method it can be observed that the fragmentation process induced by irradiation in thymic cells occurs in a fraction of the population, thus indicating that this process is not evenly distributed over the total population, and that it corresponds to a fast phenomenon in which the cells suddenly lose DNA material.
Ojeda
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F
Department of Physics, Universidad Austral de Chile, Valdivia.
Guarda
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MI
Maldonado
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Folch
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H
D000165:Acridine Orange; D000818:Animals; D016923:Cell Death; D004247:DNA / Q000032:analysis* / Q000528:radiation effects; D004249:DNA Damage / Q000528:radiation effects; D004587:Electrophoresis, Agar Gel; D005434:Flow Cytometry / Q000379:methods*; D051379:Mice; D013601:T-Lymphocytes / Q000737:chemistry* / Q000528:radiation effects
D004247:DNA; D000165:Acridine Orange