Proliferation rates in epidermis of patients with graft-versus-host disease, non-specific inflammation and normal skin.
There is evidence that epidermal stem cells or their early progeny may be the targets in graft-versus-host disease (GVHD). The early progeny should be a proliferating group of daughter cells more concentrated in areas just above or adjacent to stem cell regions. We have, therefore, compared proliferative rates of keratinocytes within relevant subregions of the epidermis using the AgNOR stain on biopsies from patients with GVHD, non-specific inflammatory infiltrates (NSI) and normal skin. We concurrently evaluated T cell infiltration using immunohistology on paraffin-embedded tissue with UCHL-1 antibody. Fifty-one bone marrow transplant patients were evaluated in each of three temporal groups (days 7-20 post-transplant, days 20-40 post-transplant, and days 80-100 post-transplant). In each of these groups, 5-9 patients with a histological diagnosis of GVHD were compared to similar numbers of patients with a histological diagnosis of non-specific dermatitis.(ABSTRACT TRUNCATED AT 250 WORDS)
Jakic-Razumovic
J
J
Department of Pathology, University Hospital Rebro, Zagreb, Yugoslavia.
Browne
M D
MD
Sale
G E
GE
D016026:Bone Marrow Transplantation / Q000009:adverse effects / Q000276:immunology / Q000473:pathology; D002455:Cell Division; D003872:Dermatitis / Q000276:immunology / Q000473:pathology; D006086:Graft vs Host Disease / Q000276:immunology / Q000473:pathology*; D006801:Humans; D009697:Nucleolus Organizer Region / Q000473:pathology; D012867:Skin / Q000276:immunology / Q000473:pathology*; D013601:T-Lymphocytes / Q000276:immunology / Q000473:pathology*