Functional characterisation of serum DNase I in MRL-lpr/lpr mice.
The autosomal defect in Fas antigen leads CD4-CD8-T-cells to accumulate in lymph nodes and spleen of MRL-lpr/lpr mice. MRL-lpr/lpr mice present increased levels of DNase I as compared to the control strain MRL-+/+. This DNase I, which most probably originates from the accumulated CD4-CD8-T-cells, cleaves nuclear DNA with a strong preference for internucleosomal sites yielding, in the presence of both Ca2+ and Mg2+, a pattern of fragments typical for apoptosis. Furthermore, we show that this "apoptosis-ladder" can be obtained with purified DNase I in presence of normal serum.
Peitsch
M C
MC
Institute of Biochemistry, University of Lausanne, Epalinges, Switzerland.
Hesterkamp
T
T
Polzar
B
B
Mannherz
H G
HG
Tschopp
J
J
D000818:Animals; D015704:CD4 Antigens / Q000032:analysis; D016827:CD8 Antigens / Q000032:analysis; D002118:Calcium / Q000494:pharmacology; D016923:Cell Death; D002467:Cell Nucleus / Q000201:enzymology*; D004247:DNA / Q000302:isolation & purification / Q000378:metabolism; D003850:Deoxyribonuclease I / Q000097:blood* / Q000302:isolation & purification / Q000378:metabolism; D007700:Kinetics; D008198:Lymph Nodes / Q000201:enzymology; D008274:Magnesium / Q000494:pharmacology; D051379:Mice; D008817:Mice, Mutant Strains; D009687:Nuclear Proteins / Q000302:isolation & purification; D010306:Parotid Gland / Q000201:enzymology; D012016:Reference Values; D016176:T-Lymphocyte Subsets / Q000201:enzymology*
D015704:CD4 Antigens; D016827:CD8 Antigens; D009687:Nuclear Proteins; D004247:DNA; D003850:Deoxyribonuclease I; D008274:Magnesium; D002118:Calcium