Characterization of lymphocyte subpopulations in cord blood.
27 cord blood samples from healthy newborns were processed according to a "whole blood" flow cytometric analysis. The CD3-positive T cells are characterized by their variability: 44.8 +/- 13.3% of lymphocytes with a lower expression of the gamma delta T cell receptor. The majority of the CD3+ cells are CD38+. Newborn T cells have less ability than adult T cells to express IL-2 receptors as well as HLA-DR. The CD4-positive T cells are equal to 31.0 +/- 10.8% of lymphocytes with a great prevalence of the CD4+/CD45RA+ population. The CD3+/CD8+/CD11b+ cells are increased to 23.4 +/- 7.1% of lymphocytes. The CD57 antigen is not expressed. The NK population, CD16+/CD56+ is increased to 25 +/- 11% of lymphocytes. 68% of CD19+ cord blood B lymphocytes coexpressed CD5. Thus the "suppressive" and "naïve" cells are prominently represented in cord blood.
Rabian-Herzog
C
C
Laboratory of Immunology and Histocompatibility, Hôpital Saint-Louis, Paris, France.
Lesage
S
S
Gluckman
E
E
D015703:Antigens, CD / Q000032:analysis; D005312:Fetal Blood / Q000166:cytology*; D006801:Humans; D007231:Infant, Newborn; D007958:Leukocyte Count; D016131:Lymphocyte Subsets; D008222:Lymphokines / Q000097:blood; D012016:Reference Values
D015703:Antigens, CD; D008222:Lymphokines