title: The study of leucine and methionine transport in the gut of rats intoxicated with thiram.
abstract: The effects of thiram on amino acids transport in rats jejunum were investigated. Using the gut everted sac method it was shown that 4 weeks intoxication with the pesticide (5% DL50 per os daily) caused time dependent inhibition in transport of C14-leucine and C14-methionine in the rat jejunum. In order to obtain further information, the influx of both amino acids into the jejunum slices was investigated in rats treated with thiram for 2 weeks. Over a range of amino acid concentrations (0-20 mM) the influx of these compounds was defined as the sum of a saturable term (active transport) and a linear term (passive transport). It was shown that thiram affected only the active transport of amino acids. The analysis of this transport for leucine using Lineweaver-Burk plots showed a decrease of the half-saturation constant (Kt), while the maximal value (Jm) remained unchanged. In the methionine transport study both kinetic constants were changed.
authors.affiliation: Department of Bromatology, Institute of Biopharmacy, Medical Academy Warsaw, Poland.
authors.forename: D
authors.initials: D
authors.lastname: Witkowska
authors.forename: L
authors.initials: L
authors.lastname: Sedrowicz
authors.forename: R
authors.initials: R
authors.lastname: Oledzka
authors.forename: E
authors.initials: E
authors.lastname: Szablicka
authors.forename: J
authors.initials: J
authors.lastname: Garszel
mesh_terms: D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D007583:Jejunum / Q000187:drug effects / Q000378:metabolism*; D007930:Leucine / Q000378:metabolism*; D008297:Male; D008715:Methionine / Q000378:metabolism*; D011897:Random Allocation; D051381:Rats; D011919:Rats, Inbred Strains; D013893:Thiram / Q000506:poisoning*
chemical_list: D013893:Thiram; D008715:Methionine; D007930:Leucine