Detection of contact sensitivity of metal salts using the murine local lymph node assay.
The local lymph node assay (LLNA) is a predictive test for the detection of contact allergens. Nickel and chromium sensitization are common cases in man. However, in a previous study topical application of nickel sulfate and potassium dichromate in aqueous solution failed to induce activation in the draining lymph node. This study describes the application of LLNA to evaluate the contact sensitivity of metal salts. The metal salts were applied in dimethylsulfoxide or aqueous ethanol solution. In some experiments, the skin of the ears was gently abraded using a needle prior to application of metal salts. The ability of seven metal salts to induce lymph node cell (LNC) proliferation was compared. Nickel, cobalt, chromium and copper salts increased LNC proliferation, whereas zinc, manganese and iron salts failed to induce LNC proliferation in this assay.
Ikarashi
Y
Y
National Institute of Hygienic Sciences, Tokyo, Japan.
Tsuchiya
T
T
Nakamura
A
A
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D003035:Cobalt / Q000633:toxicity; D003877:Dermatitis, Contact / Q000209:etiology*; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D004431:Ear, External; D000431:Ethanol / Q000494:pharmacology; D005260:Female; D005296:Ferrous Compounds / Q000633:toxicity; D008198:Lymph Nodes / Q000166:cytology / Q000187:drug effects*; D008213:Lymphocyte Activation / Q000187:drug effects; D008670:Metals / Q000633:toxicity*; D051379:Mice; D014677:Pharmaceutical Vehicles / Q000494:pharmacology; D011192:Potassium Dichromate / Q000633:toxicity; D012867:Skin / Q000187:drug effects; D012882:Skin Tests / Q000379:methods; D013431:Sulfates / Q000633:toxicity; D015032:Zinc / Q000633:toxicity; D019287:Zinc Sulfate
D005296:Ferrous Compounds; D008670:Metals; D014677:Pharmaceutical Vehicles; D013431:Sulfates; C020748:ferrous sulfate; D003035:Cobalt; D000431:Ethanol; D019287:Zinc Sulfate; C018021:cobaltous chloride; D015032:Zinc; D011192:Potassium Dichromate; D004121:Dimethyl Sulfoxide