title: Synthesis and deposition of glycosaminoglycans in the murine hemopoietic stromal line S17: modulators of the hemopoietic microenvironment.
abstract: The murine hemopoietic stromal cell line S17 can support either myelopoiesis or lymphopoiesis depending on the culture conditions (i.e., the presence of steroid or mercaptoethanol). The glycosaminoglycans are important components of the extracellular matrix, which influence hemopoietic cell proliferation. Accordingly, glycosaminoglycans have been compared under different growth conditions. Under myeloid conditions (with steroid) a higher proportion of the sulfated glycosaminoglycans was incorporated into the cell layer and the extracellular matrix was increased, whereas synthesis was reduced under lymphoid conditions (with mercaptoethanol). The inclusion of steroid or mercaptoethanol did not alter the nature of the heparan sulfate synthesized as shown by DEAE anion-exchange chromatography, cleavage with specific enzymes and resolution of the digestion products by gel electrophoresis (oligomapping), and glycosaminoglycan size. The major species of sulfated proteoglycan synthesized under the different growth conditions (200 and 110 kd for the culture supernatant and 110, 71, and 38 kd for the cell layer) were shown to be very similar by polyacrylamide gel electrophoresis. Although no qualitative difference was found biochemically between the major glycosaminoglycans/proteoglycans, scanning electron microscopy revealed major differences in the pattern of deposition of the glycosaminoglycans. Under myeloid conditions, a rich fibrous matrix covered the cell layer, whereas under lymphoid conditions glycosaminoglycan was sparsely deposited. The arrangement of the extracellular matrix may have important consequences for myelo- and lymphopoiesis.
authors.affiliation: Leukaemia Research Fund Centre, Institute of Cancer Research, London, England.
authors.forename: M
authors.initials: M
authors.lastname: Siczkowski
authors.forename: D
authors.initials: D
authors.lastname: Robertson
authors.forename: M Y
authors.initials: MY
authors.lastname: Gordon
mesh_terms: D000818:Animals; D001853:Bone Marrow / Q000502:physiology*; D001854:Bone Marrow Cells; D002460:Cell Line; D002808:Chondroitin Lyases / Q000378:metabolism; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D005109:Extracellular Matrix / Q000502:physiology; D006025:Glycosaminoglycans / Q000096:biosynthesis*; D006098:Granulocytes / Q000166:cytology; D006410:Hematopoiesis; D006854:Hydrocortisone / Q000494:pharmacology; D008214:Lymphocytes / Q000166:cytology; D008623:Mercaptoethanol / Q000494:pharmacology; D051379:Mice; D008855:Microscopy, Electron, Scanning; D011509:Proteoglycans / Q000096:biosynthesis; D013431:Sulfates / Q000378:metabolism
chemical_list: D006025:Glycosaminoglycans; D011509:Proteoglycans; D013431:Sulfates; D008623:Mercaptoethanol; D002808:Chondroitin Lyases; D006854:Hydrocortisone