title: In vitro antibody secretion by peripheral blood mononuclear cells as an expression of the immune response to Brucella spp. in humans.
abstract: We developed an assay to detect antibodies spontaneously secreted in vitro by peripheral blood mononuclear cells (PBMC) against Brucella spp. High levels of anti-Brucella immunoglobulin G (IgG) and/or IgM and/or IgA antibodies were detected in the cell supernatant solution of PBMC cultures for 12 patients suffering from acute or focalized brucellosis and for 5 patients recently vaccinated against brucellosis. This spontaneous in vitro antibody production disappeared 5 to 20 months after onset of clinical signs and 20 to 27 days after vaccination. The transient character of this anti-Brucella antibody production by PBMC is consistent with a temporary in vivo stimulation of the immune system by Brucella antigens. Detection of this secretion could improve the diagnosis of evolutive brucellosis.
authors.affiliation: Institut de Biologie, Institut National de la Santé et de la Recherche Médicale, Montpellier, France.
authors.forename: J P
authors.initials: JP
authors.lastname: Vendrell
authors.forename: A M
authors.initials: AM
authors.lastname: Conge
authors.forename: M
authors.initials: M
authors.lastname: Segondy
authors.forename: S
authors.initials: S
authors.lastname: Lombroso
authors.forename: M F
authors.initials: MF
authors.lastname: Huguet
authors.forename: A
authors.initials: A
authors.lastname: Bertrand
authors.forename: F
authors.initials: F
authors.lastname: Janbon
authors.forename: A
authors.initials: A
authors.lastname: Serre
mesh_terms: D000328:Adult; D000907:Antibodies, Bacterial / Q000032:analysis / Q000096:biosynthesis* / Q000097:blood; D000918:Antibody Specificity; D001431:Bacteriological Techniques; D002002:Brucella / Q000276:immunology*; D002006:Brucellosis / Q000175:diagnosis / Q000276:immunology*; D005069:Evaluation Studies as Topic; D006801:Humans; D066298:In Vitro Techniques; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008297:Male; D008875:Middle Aged
chemical_list: D000907:Antibodies, Bacterial