title: Metabolism of methapyrilene by Fischer-344 rat and B6C3F1 mouse hepatocytes.
abstract: 1. Suspension cultures of freshly isolated F344 rat and B6C3F1 mouse hepatocytes were compared for their ability to transform various concentrations of methapyrilene (MP). 2. MP metabolites were isolated and purified by h.p.l.c., and were identified by comparing their chromatographic and mass spectral properties with those of authentic standards. 3. Both rat and mouse hepatocytes transformed MP to tentatively identified 2-thiophenecarboxylic acid (I), and definitively identified mono-N-desmethyl methapyrilene glucuronide (II), methapyrilene glucuronide (III), methapyrilene N-oxide (V), and mono-N-desmethyl methapyrilene (VII).
authors.affiliation: National Center for Toxicological Research, Jefferson, Arkansas 72079-9502.
authors.forename: D W
authors.initials: DW
authors.lastname: Kelly
authors.forename: C L
authors.initials: CL
authors.lastname: Holder
authors.forename: W A
authors.initials: WA
authors.lastname: Korfmacher
authors.forename: T A
authors.initials: TA
authors.lastname: Getek
authors.forename: J O
authors.initials: JO
authors.lastname: Lay
authors.forename: D A
authors.initials: DA
authors.lastname: Casciano
authors.forename: J G
authors.initials: JG
authors.lastname: Shaddock
authors.forename: H M
authors.initials: HM
authors.lastname: Duhart
authors.forename: W
authors.initials: W
authors.lastname: Slikker
mesh_terms: D000818:Animals; D001711:Biotransformation; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D066298:In Vitro Techniques; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D008297:Male; D008701:Methapyrilene / Q000378:metabolism* / Q000493:pharmacokinetics; D051379:Mice; D051381:Rats; D011916:Rats, Inbred F344
chemical_list: D008701:Methapyrilene