title: Characterization of an extracellular factor that stimulates bile salt hydrolase activity in Lactobacillus sp. strain 100-100.
abstract: Bile salt hydrolase activity in Lactobacillus sp. strain 100-100 is strictly intracellular. The strain produces an extracellular factor that stimulates the intracellular hydrolase activity. The factor is inducible by conjugated bile salts, has an apparent molecular mass over 12 kDa but less than 25 kDa, is stable in air, and resistant to pronase and heat. It is partially extractable into organic solvents and inactivated by a sulphydryl group inhibitor. We postulate that the factor functions by a novel mechanism to facilitate entry of conjugated bile salts into the bacterial cells.
authors.affiliation: Department of Microbiology, University of Tennessee, Knoxville 37996.
authors.forename: S G
authors.initials: SG
authors.lastname: Lundeen
authors.forename: D C
authors.initials: DC
authors.lastname: Savage
mesh_terms: D000332:Aerobiosis; D000581:Amidohydrolases / Q000187:drug effects / Q000378:metabolism*; D019826:Cholic Acid; D002793:Cholic Acids / Q000494:pharmacology; D003956:Dialysis; D010450:Endopeptidases / Q000378:metabolism; D004789:Enzyme Activation / Q000187:drug effects; D005033:Ethylmaleimide / Q000494:pharmacology; D006000:Glycocholic Acid / Q000378:metabolism*; D006358:Hot Temperature; D007778:Lactobacillus / Q000187:drug effects / Q000201:enzymology*; D014238:Trichloroacetic Acid / Q000494:pharmacology; D014462:Ultrafiltration
chemical_list: D002793:Cholic Acids; D014238:Trichloroacetic Acid; D010450:Endopeptidases; D000581:Amidohydrolases; C023154:choloylglycine hydrolase; D019826:Cholic Acid; D006000:Glycocholic Acid; D005033:Ethylmaleimide