Construction of a tRNA-embedded-ribozyme trimming plasmid.
We have combined Cotten and Birnstiel's tRNA-embedded ribozymes and our 5'- and 3'-trimming system. Although the activity of the tRNA-embedded ribozyme was ca. 30% lower than those of naked ribozymes, since the stability of the former in bovine serum was higher than those of naked ribozymes, the tRNA-embedded ribozymes appear useful especially when the 5'- and 3'-trimming units are concatenated in tandem.
Yuyama
N
N
Fermentation Research Institute, Agency of Industrial Science & Technology, MITI, Tsukuba Science City, Japan.
Ohkawa
J
J
Inokuchi
Y
Y
Shirai
M
M
Sato
A
A
Nishikawa
S
S
Taira
K
K
D001483:Base Sequence; D004247:DNA / Q000235:genetics* / Q000378:metabolism; D005821:Genetic Techniques; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides; D010957:Plasmids; D016337:RNA, Catalytic / Q000378:metabolism*; D012343:RNA, Transfer / Q000235:genetics / Q000378:metabolism*; D015183:Restriction Mapping; D013698:Templates, Genetic
D009838:Oligodeoxyribonucleotides; D016337:RNA, Catalytic; D004247:DNA; D012343:RNA, Transfer