Distribution of 486P 3/12 antigen, ABO(H) blood group antigen and T antigen in cystectomy specimens from patients with stage T2 transitional cell carcinoma of the bladder.
We used monoclonal antibody 486P 3/12, anti-ABO(H) antibodies and anti-T antigen lectins to detect malignant transformation in cystectomy specimens from patients with stage T2 transitional cell carcinoma of the bladder. We used an immunoperoxidase technique to do extensive chessboard-like mapping studies in 6 cystectomy bladders with stage T2 lesions and 1 normal bladder from a multiorgan donor as control to characterize antigen expression. Increased 486P 3/12 antigen expression, decreased ABO(H) expression and T antigen deletion were detectable not only in the tumor area but also in tumor-surrounding areas classified as benign. We believe that cells with abnormal antigen expression are a source of tumor recurrence and can be identified with our method of quantitative immunocytology.
Heinzer
H
H
Department of Urology, University Clinic, Eppendorf, Hamburg, Federal Republic of Germany.
Huland
E
E
Mönk
M
M
Huland
H
H
D000017:ABO Blood-Group System / Q000032:analysis*; D000368:Aged; D000911:Antibodies, Monoclonal / Q000032:analysis*; D000957:Antigens, Viral, Tumor / Q000032:analysis*; D002295:Carcinoma, Transitional Cell / Q000737:chemistry / Q000473:pathology*; D015653:Cystectomy; D006801:Humans; D008875:Middle Aged; D009367:Neoplasm Staging; D001749:Urinary Bladder Neoplasms / Q000737:chemistry / Q000473:pathology*
D000017:ABO Blood-Group System; D000911:Antibodies, Monoclonal; D000957:Antigens, Viral, Tumor