title: Interaction of mouse ectoplacental cone trophoblast and uterine decidua in vitro.
abstract: During the peri-implantation stages of mouse development, the secondary trophoblast invades into the uterine decidua. This uniquely controlled invasive process results in the formation of the placenta. We have analyzed this process in vitro using cultures of decidua and microdissected ectoplacental cones from Day 7 embryos. The results showed that the interaction between these two cell types is comparable to that seen in vivo. Morphologically, the decidua maintained close contact with the spreading trophoblast, limiting its invasion and producing a multilayered trophoblast outgrowth. Attachment to the decidua was not mediated through cell-matrix binding, but the subsequent invasion into the decidua was dependent on normal matrix interactions. Secretion of proteinases by the trophoblast also seemed to be a requirement for successful invasion, but not attachment.
authors.affiliation: Department of Biological Sciences, Rutgers University, Piscataway, New Jersey 08855.
authors.forename: B S
authors.initials: BS
authors.lastname: Babiarz
authors.forename: L C
authors.initials: LC
authors.lastname: Romagnano
authors.forename: G M
authors.initials: GM
authors.lastname: Kurilla
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D015853:Cysteine Proteinase Inhibitors / Q000494:pharmacology; D003656:Decidua / Q000166:cytology* / Q000648:ultrastructure; D010064:Embryo Implantation / Q000502:physiology; D005260:Female; D006020:Glycopeptides / Q000494:pharmacology; D007976:Leupeptins / Q000494:pharmacology; D051379:Mice; D008855:Microscopy, Electron, Scanning; D009842:Oligopeptides / Q000494:pharmacology; D014327:Trophoblasts / Q000166:cytology* / Q000187:drug effects / Q000648:ultrastructure
chemical_list: D015853:Cysteine Proteinase Inhibitors; D006020:Glycopeptides; D007976:Leupeptins; D009842:Oligopeptides; C081571:tyrosyl-isoleucyl-glycyl-seryl-arginine; C032854:leupeptin; C008890:phosphoramidon