Simple, rapid and selective method using high-performance liquid chromatography for the determination of bretylium in plasma.
A high-performance liquid chromatographic method with ultraviolet detection has been developed for the determination of bretylium in plasma. Following a single-step solid-phase extraction procedure, bretylium is selectively isolated and well recovered from plasma. The assay sensitivity is 0.156 micrograms/ml from 250-microliters plasma samples and its linearity was assessed up to 40 micrograms/ml. The method is accurate (101.0 +/- 5.4%) and precise (maximum coefficient of variation of 8%). It provides a simple and time-saving alternative to existing methods and is particularly suitable for pharmacokinetic studies.
Théorêt
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Y
Département de Pharmacologie, Faculté de Médicine, Université de Montréal, Canada.
Varin
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F
D000818:Animals; D001949:Bretylium Compounds / Q000097:blood* / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D013552:Swine; D014466:Ultraviolet Rays
D001949:Bretylium Compounds; C045166:bretylium