[New perspectives of in vitro kallikrein treatment. II. Study of the hamster ovum penetration test in relation to the kallikrein-kinin system].
A significant enhancement of the fertilizing capacity of human spermatozoa by Kallikrein has recently been described in a former study. To assess the therapeutic possibilities it would be of great interest to know more about the biochemical effect of Kallikrein on the fertilizing capacity of human spermatozoa. This fact made the authors investigate 64 semen samples in which the binding score and the fertilizing capacity were determined after application of Kallikrein in a HOP-test (hamsterovum-penetration-test) and compared to the control. Furthermore these results were compared to the varying concentrations of the components of the Kallikrein-Kinin-system. An application of Kallikrein in the swim-up preparation resulted in a higher motility in patients with asthenozoospermia, whereas the patients belonging to the group of oligoasthenozoospermia showed a lower progressive motility compared to the control. The binding score (number of affixed spermatozoa) showed nearly the results in both preparations. On the other hand the fertilizing capacity in the Kallikrein preparation was significantly higher. A possible connection with the concentrations of the components of seminal plasma has not been found, yet, but regarding all results a direct enzymatic effect of Kallikrein on fertilizing potential can be assumed.
Sterzik
K
K
Frauenklinik der Universität Ulm.
Schmid
U
U
Strehler
E
E
Rosenbusch
B
B
Schill
W B
WB
Lauritzen
C
C
D000818:Animals; D006224:Cricetinae; D005260:Female; D005306:Fertilization / Q000502:physiology*; D006801:Humans; D007248:Infertility, Male / Q000503:physiopathology; D016234:Kallikrein-Kinin System / Q000502:physiology*; D008297:Male; D009845:Oligospermia / Q000503:physiopathology; D013075:Sperm Capacitation / Q000502:physiology; D013081:Sperm Motility / Q000502:physiology; D013084:Sperm-Ovum Interactions / Q000502:physiology*
Neue Perspektiven der In-vitro-Kallikrein-Behandlung II. Unterschung im Hamsterova-Pentrationstest in Abhängigkeit vom Kallikrein-Kinin-System.