Production and properties of novel human thyroid cancer specific monoclonal antibodies.
Monoclonal antibodies (TCM-7, -9 and -12) against human thyroid differentiated cancers were established by screening with human thyroid cancers, normal and benign thyroid tissue, and normal human serum protein. A monoclonal antibody (TCM-9) with strong specificity for human thyroid cancer but not for Graves' disease, adenoma or normal thyroid, was shown to recognize a 300 K protein but not to bind to native or mature human thyroglobulin. When TCM-9 was used in immunohistochemical staining tests on more than 30 types of non-thyroid lesions, no reactivity of TCM-9 was observed except with skin immature teratoma, lip squamous carcinoma and stomach adenocarcinoma, which revealed weak reactivities. TCM-9 also showed strong reactivity with two undifferentiated thyroid cancer cell lines and one tissue specimen. Thus TCM-9 is a novel monoclonal antibody against the thyroid cancer.
Sugawa
H
H
Department of Laboratory Medicine, Kyoto University, Japan.
Miyamoto
M
M
Higuchi
K
K
Smith
E
E
Mori
T
T
Imura
H
H
D000230:Adenocarcinoma / Q000276:immunology; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology; D000918:Antibody Specificity; D000951:Antigens, Neoplasm / Q000276:immunology*; D001324:Autoantigens / Q000276:immunology; D002291:Carcinoma, Papillary / Q000276:immunology; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007150:Immunohistochemistry; D007453:Iodide Peroxidase; D033862:Iron-Binding Proteins; D051379:Mice; D008807:Mice, Inbred BALB C; D013954:Thyroglobulin / Q000276:immunology; D013964:Thyroid Neoplasms / Q000276:immunology*; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D001324:Autoantigens; D033862:Iron-Binding Proteins; D013954:Thyroglobulin; C056658:TPO protein, human; D007453:Iodide Peroxidase