title: Intestinal permeability in pigs during rotavirus infection.
abstract: Macromolecular permeability of the small intestine was tested in four 3-week-old gnotobiotic pigs inoculated with porcine rotavirus strain RV277 (group A). Pigs were administered 125I-labeled polyvinylpyrrolidone (molecular weight [mol wt], 40,000) orally 1 day before and 2 and 24 hours after virus inoculation, and blood samples were obtained every 6 hours. Eight hours after rotavirus inoculation, pigs had watery diarrhea. Increased permeation of 125I-labeled polyvinylpyrrolidone was not observed after clinical signs of infection had developed. Serum total protein and urea nitrogen concentrations increased slightly at the end of the study, probably as a consequence of dehydration. Differences in blood glucose concentration were not seen. At 48 hours after viral inoculation, macromolecular permeability was tested morphologically by injecting horseradish peroxidase (mol wt, 40,000) into the jejunal lumen just distally to the ligamentum colicoduodenale. After an incubation period of 20 minutes, small segments of jejunum were obtained for stereomicroscopic, histologic, and ultrastructural investigations. Moderate hyperregenerative villus atrophy was found. Ultrastructural changes of the villus epithelium were minor, and increased macromolecular permeation was not observed.
authors.affiliation: Clinic for Large Animal Medicine, State University of Utrecht, The Netherlands.
authors.forename: L
authors.initials: L
authors.lastname: Vellenga
authors.forename: H J
authors.initials: HJ
authors.lastname: Egberts
authors.forename: T
authors.initials: T
authors.lastname: Wensing
authors.forename: J E
authors.initials: JE
authors.lastname: van Dijk
authors.forename: J M
authors.initials: JM
authors.lastname: Mouwen
authors.forename: H J
authors.initials: HJ
authors.lastname: Breukink
mesh_terms: D000818:Animals; D001284:Atrophy; D001786:Blood Glucose / Q000032:analysis; D001798:Blood Proteins / Q000032:analysis; D001806:Blood Urea Nitrogen; D005856:Germ-Free Life; D006454:Hemoglobins / Q000032:analysis; D007413:Intestinal Mucosa / Q000648:ultrastructure; D007421:Intestine, Small / Q000378:metabolism* / Q000648:ultrastructure; D007583:Jejunum / Q000473:pathology / Q000648:ultrastructure; D008854:Microscopy, Electron; D008871:Microvilli / Q000473:pathology / Q000648:ultrastructure; D010539:Permeability; D011205:Povidone / Q000493:pharmacokinetics; D012400:Rotavirus Infections / Q000097:blood / Q000503:physiopathology / Q000662:veterinary*; D013552:Swine; D013553:Swine Diseases / Q000097:blood / Q000503:physiopathology*
chemical_list: D001786:Blood Glucose; D001798:Blood Proteins; D006454:Hemoglobins; D011205:Povidone