title: [Determination and stability studies of urea in urea creams by high-performance liquid chromatography].
abstract: A method was developed for determining urea in urea creams by high-performance liquid chromatography using strong acidic cation exchange gel. Elution was carried out with 0.05 M phosphate buffer (pH 3.4) at 40 degrees C, and detection was made with a UV-spectrometer at 200 nm. This method was used for studying and comparing the stability of urea creams produced by ourselves and by other manufactures. The assay showed that there were differences in the stability of the tested creams.
authors.affiliation: Analytical Research Laboratories, Fujisawa Pharmaceutical Co., Ltd., Osaka, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Yasuda
authors.forename: T
authors.initials: T
authors.lastname: Akama
authors.forename: H
authors.initials: H
authors.lastname: Ikawa
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D009824:Ointments; D014508:Urea / Q000032:analysis*
chemical_list: D009824:Ointments; D014508:Urea