title: Enteropathogenicity of non-toxigenic Vibrio cholerae O1 for adult mice.
abstract: The enteropathogenic potential of 32 Vibrio cholerae O1 isolates that do not produce cholera toxin was examined in the orally inoculated, sealed adult mouse model. Live cultures (2 x 10(10) cfu/ml) of 7/16 clinical and 6/16 environmental isolates produced a positive intestinal fluid accumulation (FA) ratio that reached near maximum at approximately 5 h post-inoculation. Colony hybridization did not detect genes for cholera toxin, Escherichia coli heat-labile and heat-stable toxins, or shiga-like toxins. FA activity did not correlate precisely with cytotoxic activities on Chinese hamster ovary (28/32 positive), Vero (29/32) or HeLa (25/32) cells. Certain clinical and environmental isolates of non-toxigenic V. cholerae O1 appear to be enteropathogenic for the mouse, providing evidence that they may have pathogenic potential for humans through an as yet undefined mechanism(s).
authors.affiliation: Laboratory of Biological Sciences, Georgia State University, Atlanta 30303.
authors.forename: M
authors.initials: M
authors.lastname: Moyenuddin
authors.forename: K
authors.initials: K
authors.lastname: Wachsmuth
authors.forename: S H
authors.initials: SH
authors.lastname: Richardson
authors.forename: W L
authors.initials: WL
authors.lastname: Cook
mesh_terms: D000818:Animals; D016466:CHO Cells; D002771:Cholera / Q000382:microbiology*; D002772:Cholera Toxin / Q000032:analysis* / Q000235:genetics; D006224:Cricetinae; D004195:Disease Models, Animal; D006367:HeLa Cells; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism* / Q000382:microbiology; D051379:Mice; D014709:Vero Cells; D014734:Vibrio cholerae / Q000472:pathogenicity*
chemical_list: D002772:Cholera Toxin