Basic fibroblast growth factor (bFGF) immunolocalization in the rodent outer retina demonstrated with an anti-rodent bFGF antibody.
The distribution of bFGF in mouse and rat retinas was established using an antibody generated against a rat specific bFGF sequence. The patterns established with this antibody were distinctly different in comparison to the immunostaining patterns using antibodies prepared against similar regions of bovine and human bFGF sequences. Using the anti-rat antibody, at postpartum day 5, both species demonstrate bFGF-like labeling in the outer retina, primarily associated with developing photoreceptors. The anti-rat bFGF antibody in the adult mouse produces intense labeling at the level of the photoreceptor outer segment, whereas in the adult rat, Müller's cells and RPE were intensely labeled but the photoreceptors appeared to be unlabeled. In contrast, the bovine and human antibodies consistently label ganglion cells in both mouse and rat retinas, cells in the inner aspect of the inner nuclear layer and developing horizontal cells, whereas no photoreceptor labeling was observed. These results suggest that minor species differences in a short segment of the bFGF molecule used to generate these antibodies may result in major differences in apparent bFGF-like immunolocalization.
Gao
H
H
Cullen Eye Institute, Baylor College of Medicine, Houston, TX 77030.
Hollyfield
J G
JG
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies; D002417:Cattle; D016222:Fibroblast Growth Factor 2 / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D007158:Immunologic Techniques; D051379:Mice / Q000276:immunology*; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D010857:Pigment Epithelium of Eye / Q000378:metabolism; D011817:Rabbits; D051381:Rats / Q000276:immunology*; D011919:Rats, Inbred Strains; D012160:Retina / Q000166:cytology / Q000378:metabolism*; D014018:Tissue Distribution
D000906:Antibodies; D016222:Fibroblast Growth Factor 2