Calculation of antibody and antigen concentrations from ELISA data using a graphical method.
A graphical method for determining the concentration of either the antibody or the antigen from ELISA data is presented in the form of a GWBASIC program. In the program, ELISAEQ, optical densities (OD) obtained from a 96-well ELISA plate can be input either directly by interfacing a microplate reader to the computer or manually. The program uses standard sample data, and selects the semilogarithmic linear range. Over this range, a least-squares method is used to determine the concentrations of interest. In addition, a hyperbolic interpolation formula is derived over the entire range for estimating the antibody or antigen concentration of the unknown samples whose OD is beyond the linear range.
Raghava
G P
GP
Institute of Microbial Technology, Sector 39A, Chandigarh, India.
Joshi
A K
AK
Agrewala
J N
JN
D000906:Antibodies / Q000032:analysis*; D000941:Antigens / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D012984:Software
D000906:Antibodies; D000941:Antigens