Process monitoring by flow-injection immunoassay. Evaluation of a sequential competitive binding assay.
A new variation on the theme of flow-injection binding assays is presented, namely the sequential competitive binding assay, in which the sample containing the native antigen is first introduced into the flow system followed by a pulse of labelled antigen. The flow-injection binding assay was used in monitoring the effluent from column chromatographic separations of proteins. By applying a computer-based evaluation system, concentrations are automatically read and compensation is made for denaturation in the affinity sorbent.
Nilsson
M
M
Department of Biotechnology, Lund University, Sweden.
Håkanson
H
H
Mattiasson
B
B
D001667:Binding, Competitive; D002850:Chromatography, Gel; D017022:Flow Injection Analysis / Q000379:methods*; D006735:Horseradish Peroxidase; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D007136:Immunoglobulins / Q000276:immunology; D011489:Protein Denaturation; D012709:Serum Albumin / Q000276:immunology
D007136:Immunoglobulins; D012709:Serum Albumin; D006735:Horseradish Peroxidase