Enzyme immunoassay and immunochemical characterization of pancreatic stone protein in human serum.
Monoclonal antibodies were raised against human pancreatic stone protein (PSP) and used for one-step enzyme immunoassay (EIA). PSP-S2-5 was employed as the standard in the assay. The assay's measurable range was 25-1,500 ng/ml and within run coefficient of variation was 3.7-6.4%. Analytical recovery of the assay was 101.5 +/- 5.65% (mean +/- SD). The results of experiments in which serum was fractionated by Mono S (cation exchange chromatography) suggested that most of immunoreactive material in human serum is PSP-S2-5. The EIA offers simple, rapid, and specific analysis of serum PSP level for clinical diagnosis.
Katsuzaki
T
T
Department of Clinical Chemistry, Maruko Pharmaceutical Co., Ltd., Kasugai, Japan.
Tatemichi
N
N
Takeichi
C
C
Hayakawa
S
S
Hayakawa
T
T
Shibata
T
T
Nakae
Y
Y
Naruse
S
S
D000911:Antibodies, Monoclonal; D002135:Calcium-Binding Proteins / Q000097:blood* / Q000276:immunology / Q000592:standards; D002852:Chromatography, Ion Exchange; D005069:Evaluation Studies as Topic; D006801:Humans; D007120:Immunochemistry; D007124:Immunoenzyme Techniques / Q000592:standards / Q000706:statistics & numerical data; D051847:Lithostathine; D009419:Nerve Tissue Proteins; D010190:Pancreatic Neoplasms / Q000097:blood; D010195:Pancreatitis / Q000097:blood; D012015:Reference Standards; D012680:Sensitivity and Specificity
D000911:Antibodies, Monoclonal; D002135:Calcium-Binding Proteins; D051847:Lithostathine; D009419:Nerve Tissue Proteins; C497617:REG1A protein, human