title: Efficiency of homologous intermolecular recombination at different locations on the Bacillus subtilis chromosome.
abstract: The efficiencies of intermolecular recombination at 12 different locations on the Bacillus subtilis chromosome were determined by transforming competent cells with a nonreplicative plasmid. The efficiencies varied by only about threefold but were significantly different (P less than 0.05 by a chi-square test) for approximately 20% of the locations. The recA gene product is required for recombination, and the addA gene product appears to affect the variation in a site-specific way.
authors.affiliation: Laboratoire de Génétique Microbienne, Institut National de la Recherche Agronomique, Jouy en Josas, France.
authors.forename: I
authors.initials: I
authors.lastname: Biswas
authors.forename: V
authors.initials: V
authors.lastname: Vagner
authors.forename: S D
authors.initials: SD
authors.lastname: Ehrlich
mesh_terms: D001412:Bacillus subtilis / Q000235:genetics*; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial / Q000648:ultrastructure; D004269:DNA, Bacterial / Q000235:genetics; D043211:Exodeoxyribonuclease V; D005090:Exodeoxyribonucleases / Q000235:genetics; D011938:Rec A Recombinases / Q000235:genetics; D011995:Recombination, Genetic
chemical_list: D004269:DNA, Bacterial; D011938:Rec A Recombinases; D005090:Exodeoxyribonucleases; D043211:Exodeoxyribonuclease V