Triple-label beta liquid scintillation counting.
The detection of radioactive compounds by liquid scintillation has revolutionized modern biology, yet few investigators make full use of the power of this technique. Even though multiple isotope counting is considerably more difficult than single isotope counting, many experimental designs would benefit from using more than one isotope. The development of accurate isotope counting techniques enabling the simultaneous use of three beta-emitting tracers has facilitated studies in our laboratory using the multiple tracer indicator dilution technique for assessing rates of transmembrane transport and cellular metabolism. The details of sample preparation, and of stabilizing the liquid scintillation spectra of the tracers, are critical to obtaining good accuracy. Reproducibility is enhanced by obtaining detailed efficiency/quench curves for each particular set of tracers and solvent media. The numerical methods for multiple-isotope quantitation depend on avoiding error propagation (inherent to successive subtraction techniques) by using matrix inversion. Experimental data obtained from triple-label beta counting illustrate reproducibility and good accuracy even when the relative amounts of different tracers in samples of protein/electrolyte solutions, plasma, and blood are changed.
Bukowski
T R
TR
Center for Bioengineering, University of Washington, Seattle 98195.
Moffett
T C
TC
Revkin
J H
JH
Ploger
J D
JD
Bassingthwaighte
J B
JB
D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D005069:Evaluation Studies as Topic; D010649:Phenylalanine / Q000097:blood; D011817:Rabbits; D011868:Radioisotopes; D015203:Reproducibility of Results; D012588:Scintillation Counting / Q000379:methods* / Q000706:statistics & numerical data; D012709:Serum Albumin / Q000032:analysis
D001786:Blood Glucose; D011868:Radioisotopes; D012709:Serum Albumin; D010649:Phenylalanine