A general, PCR-based method for single or combinatorial oligonucleotide-directed mutagenesis on pUC/M13 vectors.
Merino
E
E
Instituto de Biotecnología, UNAM, Cuernavaca, Morelos, Mexico.
Osuna
J
J
Bolívar
F
F
Soberón
X
X
D001483:Base Sequence; D004926:Escherichia coli; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009841:Oligonucleotides / Q000235:genetics*; D010957:Plasmids; D016133:Polymerase Chain Reaction / Q000379:methods*; D014170:Transformation, Genetic
D009841:Oligonucleotides