Activated peripheral blood mononuclear cells in IgA nephropathy express platelet-derived growth factor B-chain messenger RNA.
We investigated platelet-derived growth factor (PDGF) A- and B-chain messenger RNA expression in peripheral blood mononuclear cells or T cells obtained from 26 patients with IgA nephropathy and 15 healthy age-matched control subjects. Most patients with IgAN (88%) showed elevated PDGF B-chain expression in peripheral blood mononuclear cells; no PDGF B-chain expression was detected in peripheral blood mononuclear cells of normal control subjects. In T cells from both patients with IgAN and normal control subjects, however, PDGF B-chain messenger RNA expression was not detected. A positive correlation was noted between PDGF B-chain messenger RNA levels and urinary protein excretion. The PDGF B-chain messenger RNA expression in peripheral blood mononuclear cells also correlated with histopathologic changes in renal tissue obtained from patients with IgAN. Sixty-five percent of patients with grade III or IV histopathologic findings showed strong PDGF B-chain gene expression in their peripheral blood mononuclear cells. We could not detect PDGF A-chain messenger RNA expression in either peripheral blood mononuclear cells and T cells obtained from patients with IgAN and from healthy control subjects. These studies suggest that abnormally regulated PDGF B-chain expression in peripheral blood mononuclear cells may be associated with the progression of IgAN and may be useful as an indicator of disease activity.
Nakamura
T
T
Department of Medicine, Juntendo University School of Medicine, Tokyo, Japan.
Ebihara
I
I
Nagaoka
I
I
Tomino
Y
Y
Koide
H
H
D000328:Adult; D015342:DNA Probes; D005922:Glomerulonephritis, IGA / Q000097:blood* / Q000235:genetics / Q000473:pathology; D006801:Humans; D009000:Monocytes / Q000502:physiology*; D009393:Nephritis / Q000097:blood / Q000235:genetics; D010982:Platelet-Derived Growth Factor / Q000096:biosynthesis* / Q000235:genetics*; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000235:genetics; D012016:Reference Values; D013601:T-Lymphocytes / Q000502:physiology*
D015342:DNA Probes; D010982:Platelet-Derived Growth Factor; D012333:RNA, Messenger