High-performance liquid chromatographic determination of free polyamines in human seminal plasma.
Putrescine, spermidine and spermine were determined quantitatively in 192 ultrafiltered samples of human seminal plasma with a precision of +/- 1.9% by pre-column derivatization with benzoyl chloride and reversed-phase chromatography. The concentrations of free putrescine, spermidine and spermine ranged from 0 to 1.96 mmol/l (mean = 0.10), 0.017-0.96 mmol/l (mean = 0.16) and 0.13 to 20.80 mmol/l (mean = 2.43), respectively. A significant correlation between the amounts of spermidine and spermine was found (r2 = 0.806, P less than 0.0005). Otherwise, no significant correlations between free-polyamine levels and routine spermiogram parameters such as volume, sperm count, motility, vitality and morphology were obtained.
Oefner
P J
PJ
Department of Urology, Leopold-Franzens-University, Innsbruck, Austria.
Wongyai
S
S
Bonn
G
G
D000293:Adolescent; D000328:Adult; D015317:Biogenic Polyamines / Q000032:analysis*; D001826:Body Fluids / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D008297:Male; D008875:Middle Aged; D011700:Putrescine / Q000032:analysis; D012661:Semen / Q000737:chemistry*; D013095:Spermidine / Q000032:analysis; D013096:Spermine / Q000032:analysis; D013696:Temperature; D014462:Ultrafiltration
D015317:Biogenic Polyamines; D013096:Spermine; D013095:Spermidine; D011700:Putrescine