Recombinant human interleukin 1 beta and tumor necrosis factor affect glycosylation of serum alpha 1-acid glycoprotein in rats.
Serum concentration and glycosylation of rat alpha 1-acid glycoprotein (alpha 1-AGP) were evaluated after the in vivo administration of recombinant human interleukin-1 beta (rhIL-1 beta) and tumor necrosis factor alpha (rhTNF-alpha), alone or associated. The effect of LPS and turpentine was also studied. In all models, serum alpha 1-AGP concentrations were increased and glycosylation was altered. The alpha 1-AGP levels reached 1.8 g/liter with cytokines alone, 2.1 g/liter with cytokines associated or LPS, and 3.4 g/liter with turpentine. Analysis by concanavalin A (Con A) affinoimmunoelectrophoresis (CAIE) revealed that the relative proportion of Con A unreactive form always decreased whatever the inducing agent. On the other hand, the resulting effect on the concentrations of Con A unreactive alpha 1-AGP concentrations was an increase with cytokines alone or LPS and a decrease with cytokines associated or turpentine. These results suggest a dissociation between the alteration in the level of alpha 1-AGP synthesis and in the pattern of its glycosylation in the various inflammatory models.
Poüs
C
C
Département de Biochimie Générale, UFR des Sciences Pharmaceutiques et Biologiques (Université Paris XI), Châtenay-Malabry, France.
Chauvelot-Moachon
L
L
Lecoustillier
M
M
Durand
G
G
D000210:Acute-Phase Reaction; D000818:Animals; D003208:Concanavalin A / Q000378:metabolism; D004357:Drug Synergism; D004731:Endotoxins / Q000494:pharmacology; D006031:Glycosylation / Q000187:drug effects; D006801:Humans; D007163:Immunosorbent Techniques; D007375:Interleukin-1 / Q000494:pharmacology*; D008297:Male; D009961:Orosomucoid / Q000378:metabolism*; D011499:Protein Processing, Post-Translational / Q000187:drug effects; D051381:Rats; D011916:Rats, Inbred F344; D011994:Recombinant Proteins / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*; D014425:Turpentine / Q000494:pharmacology
D004731:Endotoxins; D007375:Interleukin-1; D009961:Orosomucoid; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; D003208:Concanavalin A; C044101:endotoxin, Escherichia coli; D014425:Turpentine