Molecular cloning of a specific DNA probe for the identification of Bacillus licheniformis.
Bacillus licheniformis has been found to be one of the dominant nosocomial species of Bacillus: laboratories dealing with nosocomial infections must be able to identify Bacillus up to the species level. To date, no DNA probes have been isolated for B. licheniformis although there is a clear need for a direct detection by polymerase chain reaction. The isolation of a B. licheniformis-specific DNA probe, as described in this paper, represents the first step toward accomplishing this goal.
Bollet
C
C
Laboratoire de Microbiologie et d'Hygiène Hospitalière, Hôpital Salvator, Marseille, France.
Vignoli
C
C
De Micco
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P
D001407:Bacillus / Q000235:genetics* / Q000302:isolation & purification; D003001:Cloning, Molecular; D015342:DNA Probes / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000235:genetics*; D015723:Gene Library; D009693:Nucleic Acid Hybridization
D015342:DNA Probes; D004269:DNA, Bacterial