Identification and quantitative analysis of urinary metabolites of dichloropropanols in rats.
Urinary metabolites of dichloropropanols in rats were analyzed by gas chromatography-mass spectrometry (GC/MS). Solutions of dichloropropanols consisting of 1, 3-dichloro-2-propanol (DC2P) and 2, 3-dichloro-1-propanol (DC1P) were diluted in a saline at the concentration of 100 mg/ml, and 0.1 ml of the solutions were subcutaneously injected into male Wistar rats weighing about 160g. The urine samples were collected over a period of 24 hours after the injections. DC2P and DC1P in the urine were extracted with ethylacetate and analyzed by a GC/MS. The derivatization procedure with 4-bromophenylboric acid after acetonitril extraction was applied for the analyses of diols in the urine. By the GC/MS analysis, 3-chloro-1, 2-propanediol (3CPD), 2-chloro-1, 3-propanediol (2CPD) and 1, 2-propanediol (PPD) were identified as the hydroxylated metabolites of dichlorpropanols. Based on the analytical results, the metabolic pathways of dichlorpropanols forming 3CPD and 2CPD, and then hydroxylating to PPD were elucidated.
Koga
M
M
Research Center for Common Use, School of Medicine, University of Occupational and Environmental Health, Kitakyushu, Japan.
Inoue
N
N
Imazu
K
K
Yamada
N
N
Shinoki
Y
Y
D000818:Animals; D002728:Chlorohydrins / Q000378:metabolism* / Q000652:urine; D008401:Gas Chromatography-Mass Spectrometry; D006900:Hydroxylation; D008297:Male; D011409:Propylene Glycols / Q000652:urine; D051381:Rats; D011919:Rats, Inbred Strains; D000517:alpha-Chlorohydrin / Q000031:analogs & derivatives* / Q000378:metabolism / Q000652:urine
D002728:Chlorohydrins; D011409:Propylene Glycols; C033489:1,3-dichloro-2-propanol; C076159:2,3-dichloro-1-propanol; D000517:alpha-Chlorohydrin