Biochemical characterization of the metallo-beta-lactamase CcrA from Bacteroides fragilis TAL3636.
The CcrA beta-lactamase from Bacteroides fragilis TAL3636 was cloned into Escherichia coli and purified from inclusion bodies. This group 3 metalloenzyme hydrolyzed most beta-lactam antibiotics, including cephamycins and carbapenems. Following inhibition by chelators, enzyme activity was recovered with the cations Zn2+ and Co2+. Clavulanate and sulbactam were activators; tazobactam at 10 microM inactivated the enzyme.
Yang
Y
Y
Medical Research Division, American Cyanamid Corporation, Pearl River, New York 10965.
Rasmussen
B A
BA
Bush
K
K
D001441:Bacteroides fragilis / Q000201:enzymology*; D004591:Electrophoresis, Polyacrylamide Gel; D001618:beta-Lactamases / Q000032:analysis*
D001618:beta-Lactamases