Cleavage rate of diandric triploid mouse embryos during the preimplantation period.
The postimplantation development of human and animal triploid embryos is well documented, but there is little informative data on their preimplantation development. An analysis of cell number at appropriate times during this period and thus their cleavage rate would give an indication of the potential triploids have for further development and may explain some problems associated with their postimplantation development. To rule out any effects of technical procedures on cleavage rate, appropriate controls were used. Diandric triploid embryos were produced using standard micromanipulatory techniques, which involved the injection of a male pronucleus into a recipient one-cell-stage embryo. The karyoplast was fused to the cytoplasm by electrofusion, and the resulting tripronucleate diandric triploid embryos were transferred to appropriate pseudopregnant recipients. At specific times after the transfer, the embryos were recovered and cell numbers established. The results were plotted and regression lines drawn. Three controls were used 1) micromanipulated diploid embryos from which the male pronucleus had been removed and immediately reinserted and fused to restore diploidy, 2) diploid embryos that had been briefly incubated in cytochalasin D and colcemid to find out the effects these agents had on development, and 3) diploid embryos that had been isolated and briefly incubated in tissue culture medium. All embryos were subsequently transferred to recipients. After isolation at specific times during the preimplantation period, cell numbers were also established and the results plotted.(ABSTRACT TRUNCATED AT 250 WORDS)
Henery
C C
CC
Department of Anatomy, University Medical School, Edinburgh, United Kingdom.
Kaufman
M H
MH
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002970:Cleavage Stage, Ovum / Q000166:cytology*; D015638:Cytochalasin D / Q000494:pharmacology; D003599:Cytoskeleton / Q000187:drug effects; D003703:Demecolcine / Q000494:pharmacology; D004624:Embryo Transfer; D047108:Embryonic Development / Q000502:physiology*; D005314:Embryonic and Fetal Development / Q000235:genetics; D005260:Female; D008297:Male; D051379:Mice / Q000196:embryology*; D011123:Polyploidy; D011247:Pregnancy
D015638:Cytochalasin D; D003703:Demecolcine