title: Identification of a constitutive form of cytochrome P-450 with high activity towards macrolide antibiotics in Syrian golden hamster liver.
abstract: The presence of cytochromes P450 belonging to the P450IIIA family was investigated in the liver of untreated Syrian golden hamsters and compared to those of untreated, phenobarbital- and dexamethasone-treated rats. The microsomal preparations from untreated Syrian golden hamster livers exhibited higher activities of N-demethylation towards the macrolide antibiotics, erythromycin and troleandomycin, than those from untreated and phenobarbital-treated rats. Western blots analysis revealed larger amounts of proteins immunorelated to the rat cytochrome P450IIIA1 isozyme in Syrian golden hamster liver than in untreated and phenobarbital-treated rats. The N-demethylation of erythromycin and troleandomycin were strongly inhibited after preincubation of the microsomes from Syrian golden hamsters with anti-P450IIIA1. We conclude that a cytochrome P450 belonging to the P450IIIA family is expressed constitutively in the liver from Syrian golden hamsters, whereas it is not in rat liver.
authors.affiliation: Department of Pharmaceutical Sciences, Institute of Public Health, Tokyo, Japan.
authors.forename: E
authors.initials: E
authors.lastname: Antignac
authors.forename: M
authors.initials: M
authors.lastname: Fukuhara
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000378:metabolism*; D006224:Cricetinae; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D004917:Erythromycin / Q000378:metabolism; D007527:Isoenzymes / Q000378:metabolism; D008297:Male; D008647:Mesocricetus; D008862:Microsomes, Liver / Q000378:metabolism*; D051381:Rats; D013045:Species Specificity; D013379:Substrate Specificity; D014217:Troleandomycin / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D007527:Isoenzymes; D004917:Erythromycin; D003577:Cytochrome P-450 Enzyme System; D014217:Troleandomycin