title: Immunohistochemical localization of PGF2 alpha receptor in the rat ovary.
abstract: As a step towards understanding the role of prostaglandin F2 alpha (PGF2 alpha) in ovarian function, a rabbit antiserum against purified PGF2 alpha receptor (PGF2 alpha-R) was produced. This report details the use of this antiserum in immunohistochemical staining of ovaries of non-pregnant and pregnant rats to ascertain which cell types, in vivo, possess PGF2 alpha-R. In non-pregnant rats, three ovarian cell subpopulations contain immunoreactive PGF2 alpha-R. These include: a subpopulation of the cells found in corpora lutea, a subpopulation of the thecal cells surrounding secondary and mature (Graafian) follicles, and a subpopulation of primary and secondary interstitial cells. The ovarian tissues and cell types in which immunoreactive PGF2 alpha-R cannot be demonstrated include: the serosa overlying the ovary and its vessels, the coelomic epithelium and its underlying cortical stroma, medullary stroma and vessels, granulosa cells of primary, secondary and mature follicles, the oocyte, and the blood vessels and stroma within corpora lutea. PGF2 alpha-R immunohistochemical staining of corpora lutea from non-pregnant animals was examined both prior to the start of luteolysis and during luteolysis. During luteolysis, cells undergoing apoptosis stained for the presence of PGF2 alpha-R. PGF2 alpha-R immunohistochemical staining was also examined in corpora lutea during pregnancy and until 4 days postpartum. The major findings here were the apparent large increase in staining intensity of granulosa-lutein cells during pregnancy, and the loss of PGF2 alpha-R immunopositivity of the granulosa-lutein cells during the postpartum period. In summary, three ovarian cell subpopulations, all of which can secrete steroids, possess immunoreactive PGF2 alpha-R.
authors.affiliation: Department of Pathology, University of Colorado Health Sciences Center, Denver.
authors.forename: D J
authors.initials: DJ
authors.lastname: Orlicky
authors.forename: L
authors.initials: L
authors.lastname: Fisher
authors.forename: N
authors.initials: N
authors.lastname: Dunscomb
authors.forename: G J
authors.initials: GJ
authors.lastname: Miller
mesh_terms: D000818:Animals; D003338:Corpus Luteum / Q000737:chemistry / Q000648:ultrastructure; D015237:Dinoprost / Q000378:metabolism*; D005260:Female; D010053:Ovary / Q000737:chemistry* / Q000648:ultrastructure; D011247:Pregnancy; D051381:Rats; D011916:Rats, Inbred F344; D011982:Receptors, Prostaglandin / Q000187:drug effects* / Q000378:metabolism; D013799:Theca Cells / Q000737:chemistry / Q000648:ultrastructure
chemical_list: D011982:Receptors, Prostaglandin; C034671:prostaglandin F2alpha receptor; D015237:Dinoprost