Replication of Autographa californica baculovirus (AcMNPV) in a coleopteran cell line.
A coleopteran cell line (AGE) derived from the cotton boll weevil Anthonomus grandis supported replication of Autographa californica multiple nuclear polyhedrosis virus (AcMNPV). The titer of extracellular virus (ECV) and the number of occlusion bodies (OB) produced in AGE cells were approximately equal to those produced by a Trichoplusia ni cell line (TN-CL1), and the OB produced by both cell lines were equally infectious for T. ni larvae. The identity of the AGE cell line was established by chromosome and isoenzyme analyses.
McIntosh
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AH
USDA/ARS, Biological Control of Insects Research Lab., Columbia, Missouri 65205.
Barcenas
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D000818:Animals; D016367:Baculoviridae / Q000502:physiology* / Q000648:ultrastructure; D002460:Cell Line; D001517:Coleoptera / Q000382:microbiology*; D005956:Glucose-6-Phosphate Isomerase / Q000032:analysis; D007527:Isoenzymes / Q000032:analysis; D007915:Lepidoptera; D014779:Virus Replication
D007527:Isoenzymes; D005956:Glucose-6-Phosphate Isomerase