title: Horseradish peroxidase labeling of growth cones and axons beyond the site of injury in injured rabbit optic nerve axons growing in their own environment.
abstract: Spontaneous growth of injured axons in the mammalian central nervous system is limited. We have previously shown an apparently regenerative growth of injured optic axons in the adult rabbit, achieved by supplying them with soluble substances originating from growing axons, followed by low energy helium-neon laser irradiation. The growing unmyelinated and thinly myelinated axons were embedded in astrocytes, and some were in the process of remyelination by oligodendrocytes. They were shown to have originated from the retinal ganglion cells. The present study further supports evidence relating to the origin and nature of these axons. Light microscopic analysis of these axons labeled with anterogradely transported horseradish peroxidase revealed that many of these axons have varicosities and bear growth cone-like swellings in their tips. These axons traverse the lesion site and extend into the distal stump in a disorganized pattern.
authors.affiliation: Department of Neurobiology, Weizmann Institute of Science, Rehovot, Israel.
authors.forename: V
authors.initials: V
authors.lastname: Lavie
authors.forename: A
authors.initials: A
authors.lastname: Solomon
authors.forename: S
authors.initials: S
authors.lastname: Ben-Bassat
authors.forename: M
authors.initials: M
authors.lastname: Belkin
authors.forename: M
authors.initials: M
authors.lastname: Schwartz
mesh_terms: D000818:Animals; D001369:Axons / Q000737:chemistry*; D001692:Biological Transport / Q000502:physiology; D002347:Carps / Q000502:physiology; D002455:Cell Division / Q000502:physiology; D006735:Horseradish Peroxidase; D008854:Microscopy, Electron; D009900:Optic Nerve / Q000648:ultrastructure*; D011817:Rabbits
chemical_list: D006735:Horseradish Peroxidase