Characterization of the snake venoms from seven Brazilian species of Bothrops by FPLC anion-exchange chromatography.
1. The elution profiles and the caseinolytic, myotoxic, coagulant and hemorrhagic activities of the venoms of seven Bothrops species fractionated on a Mono-Q FPLC column were analyzed. 2. Each venom separated into 16-20 peaks, with good reproducibility and the activities were concentrated in virtually discrete regions of the chromatogram. 3. There is a considerable overlap of active proteins in the different species venoms and our results indicate that a venom pool with the species B. jararaca, B. jararacussu, B. moojeni, B. neuwiedi and B. atrox venoms would contain the major active proteins determined in the seven species.
Leite
L C
LC
Biotechnology Center, Instituto Butantan, São Paulo, Brazil.
Furtado
M F
MF
Correa
T C
TC
Raw
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I
D000818:Animals; D001938:Brazil; D002364:Caseins; D002852:Chromatography, Ion Exchange; D003435:Crotalid Venoms / Q000737:chemistry* / Q000276:immunology / Q000633:toxicity; D006470:Hemorrhage / Q000139:chemically induced; D051379:Mice; D009132:Muscles / Q000187:drug effects; D010447:Peptide Hydrolases / Q000302:isolation & purification; D011506:Proteins / Q000302:isolation & purification; D012911:Snakes; D013045:Species Specificity; D013917:Thrombin / Q000302:isolation & purification
D002364:Caseins; D003435:Crotalid Venoms; D011506:Proteins; D010447:Peptide Hydrolases; D013917:Thrombin