title: B-B10 (anti-CD25)-saporin immunotoxin--a possible tool in graft-versus-host disease treatment.
abstract: An immunotoxin containing the B-B10 MoAb, directed against the CD25 determinant, and the ribosome-inactivating protein saporin, inhibits 3H-TdR incorporation in phytohemagglutin, allogeneic-stimulated lymphocytes (primary and secondary mixed-lymphocyte reaction), and in an alloreactive T cell clone. A lower degree of inhibition was obtained with the B-B10 MoAb, which is known to inhibit IL-2 activity, as well as with the unconjugated compounds. These results suggest that the in vivo administration of the conjugate might be a more effective tool in the treatment of patients affected by graft-versus-host disease than B-B10 alone, by inducing an efficient killing of allogeneic-reacting T lymphocytes.
authors.affiliation: Istituto Nazionale per la Ricerca sul Cancro, Genoa, Italy.
authors.forename: P L
authors.initials: PL
authors.lastname: Tazzari
authors.forename: A
authors.initials: A
authors.lastname: Bolognesi
authors.forename: D
authors.initials: D
authors.lastname: De Totero
authors.forename: S
authors.initials: S
authors.lastname: Pileri
authors.forename: R
authors.initials: R
authors.lastname: Conte
authors.forename: J
authors.initials: J
authors.lastname: Wijdenes
authors.forename: P
authors.initials: P
authors.lastname: Hervé
authors.forename: M
authors.initials: M
authors.lastname: Soria
authors.forename: F
authors.initials: F
authors.lastname: Stirpe
authors.forename: M
authors.initials: M
authors.lastname: Gobbi
mesh_terms: D000911:Antibodies, Monoclonal / Q000627:therapeutic use*; D001854:Bone Marrow Cells; D006412:Hematopoietic Stem Cells / Q000276:immunology; D006801:Humans; D007156:Immunologic Memory; D007165:Immunosuppression Therapy / Q000379:methods; D000922:Immunotoxins; D066298:In Vitro Techniques; D008213:Lymphocyte Activation; D009699:N-Glycosyl Hydrolases; D010940:Plant Proteins / Q000008:administration & dosage*; D015375:Receptors, Interleukin-2 / Q000276:immunology*; D054789:Ribosome Inactivating Proteins, Type 1; D000076985:Saporins; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D000922:Immunotoxins; D010940:Plant Proteins; D015375:Receptors, Interleukin-2; D054789:Ribosome Inactivating Proteins, Type 1; D009699:N-Glycosyl Hydrolases; D000076985:Saporins