title: Cytogenetic experience in prenatal fra(X) detection on amniotic fluid cultures.
abstract: Since 1987, we have had experience with 13 prenatal diagnoses of 11 women at risk for the fragile X syndrome by cytogenetic studies on amniotic fluid cultures. The induction method included TC 199 medium and methotrexate. Results were obtained in all cases. Ten were males and three were prenatally diagnosed as being affected. Three were females and none of them was fra(X)-positive. Results were confirmed in 10/13 cases. In these cases, we had neither false-positive nor false-negative results.
authors.affiliation: Department of Haematology, Hôpital Nord, St-Priest-en-Jarez, France.
authors.forename: F
authors.initials: F
authors.lastname: Devillard
authors.forename: F
authors.initials: F
authors.lastname: Prieur
authors.forename: M
authors.initials: M
authors.lastname: Delhomme-Bachy
authors.forename: B
authors.initials: B
authors.lastname: De Freminville
authors.forename: B
authors.initials: B
authors.lastname: Lauras
authors.forename: C P
authors.initials: CP
authors.lastname: Brizard
authors.forename: J
authors.initials: J
authors.lastname: Fraisse
authors.forename: M F
authors.initials: MF
authors.lastname: Bertheas
mesh_terms: D000649:Amniocentesis; D000653:Amniotic Fluid / Q000166:cytology; D002478:Cells, Cultured; D005260:Female; D005312:Fetal Blood / Q000166:cytology; D005600:Fragile X Syndrome / Q000175:diagnosis*; D006801:Humans; D008727:Methotrexate; D011247:Pregnancy; D015203:Reproducibility of Results; D012307:Risk Factors
chemical_list: D008727:Methotrexate