[Radiosensitivity of cells-precursors of hemopoietic stroma (CFU-F) in the rat bone marrow under the effect of 60Co gamma irradiation in various conditions].
Conditions have been developed for cloning cells-precursors of rat bone marrow haemopoietic stroma, that form in culture dense and sparse fibroblast colonies (CFU-F) at a plating efficiency of 10(-4). Radiosensitivity of rat bone marrow CFU-F, with 60Co-gamma-irradiation in vitro, is characterized by the values of Do and n of 1.87 Gy and 1.4 respectively for all clones; 0.65 Gy and 6.7 for dense clones, and 4.27 Gy and 1.0 for sparse clones. This confirms the observed heterogeneity of CFU-F population consisting of highly radiosensitive and radioresistant subpopulations. The parameters of rat bone marrow CFU-F are nearly the same with irradiation both in vivo and in vitro; with in situ irradiation, the oxygen effect comes into play in a radiosensitive subpopulation of CFU-F; the OER values are 1.6, 2.6 and 0.9 for all, dense and sparse clones respectively.
Kolesnikova
A I
AI
Kal'sina
S Sh
SSh
Konopliannikov
A G
AG
Lepekhina
L A
LA
D000818:Animals; D001853:Bone Marrow / Q000528:radiation effects*; D003037:Cobalt Radioisotopes / Q000494:pharmacology*; D003114:Colony-Forming Units Assay; D005347:Fibroblasts / Q000528:radiation effects; D006412:Hematopoietic Stem Cells / Q000528:radiation effects*; D066298:In Vitro Techniques; D051381:Rats; D017208:Rats, Wistar
D003037:Cobalt Radioisotopes
Radiochuvstvitel'nost' kletok-predshestvennikov gemopoéticheskoĭ stromy (KOE-F), soderzhashchikhsia v kostnom mozge krys, pri deĭstvii gama-izlucheniia 60Co v razlichnykh usloviiakh.