Metabolic thermotolerance: magnetic resonance detected protection of glutamate synthase.
Metabolism in maize meristem cultures exposed to different heat treatments has been analyzed by 13C-NMR spectroscopy of tissue extracts. The effects of a 40 degrees C permissive stress were compared with a 45 degrees C lethal stress, and the metabolism of glutamate and glutamine were markedly altered by both temperatures. Changes in the incorporation of labeled precursors, alterations due to the in vivo application of enzyme inhibitors, and differences in the activity of enzymes in cell free extracts have confirmed that glutamate synthase (GluS) is partially inactivated by the lethal thermal exposure. This enzyme is quantitatively protected by the induction of thermotolerance. The time dependence for the protection correlates with the appearance of a set of late-arising heat shock proteins (hsps). The function of these late-arising proteins is not yet known, but only one of them, a 67-kDa protein, is spatially correlated with GluS protection. Therefore, the quantitative protection of a key metabolic enzyme has been correlated with the in vivo function of a specific hsp.
Logan
T M
TM
Department of Chemistry, University of Chicago, Illinois 60637.
Zhong
P
P
Lynn
D G
DG
D001345:Autoradiography; D002247:Carbon Isotopes; D004591:Electrophoresis, Polyacrylamide Gel; D004795:Enzyme Stability; D005970:Glutamate Synthase / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D006358:Hot Temperature; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008715:Methionine / Q000378:metabolism; D011487:Protein Conformation; D013462:Sulfur Radioisotopes; D013816:Thermodynamics; D003313:Zea mays / Q000201:enzymology
D002247:Carbon Isotopes; D013462:Sulfur Radioisotopes; D008715:Methionine; D005970:Glutamate Synthase