Flow cytometric quantitation of the expression of membrane cofactor protein as a marker for the human sperm acrosome reaction.
A mAb (TRA-2-10, IgG1) to an embryonal carcinoma cell line (2102ep) that recognizes an antigen termed membrane cofactor protein (CD 46, TLX antigen) binds to human sperm after chemical induction of acrosomal loss by Ca2+ ionophore. An indirect immunofluorescence assay was developed in which sperm membrane cofactor protein was detected by flow cytometry on acrosome-reacted living human sperm. The expression of the membrane cofactor protein antigen on acrosome-reacted sperm may represent a marker that can be used in a rapid, quantitative, and reproducible flow cytometric assay for the evaluation of human sperm acrosomal loss.
D'Cruz
O J
OJ
Department of Obstetrics and Gynecology, University of Oklahoma Health Sciences Center, Oklahoma City 73190.
Hass
G G
GG
D000177:Acrosome / Q000276:immunology / Q000502:physiology*; D000911:Antibodies, Monoclonal; D000941:Antigens / Q000032:analysis; D015703:Antigens, CD / Q000032:analysis*; D000001:Calcimycin / Q000494:pharmacology; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D006801:Humans; D008297:Male; D051916:Membrane Cofactor Protein; D008562:Membrane Glycoproteins / Q000032:analysis*; D008856:Microscopy, Fluorescence; D013094:Spermatozoa / Q000737:chemistry* / Q000276:immunology / Q000502:physiology
D000911:Antibodies, Monoclonal; D000941:Antigens; D015703:Antigens, CD; C497857:CD46 protein, human; D051916:Membrane Cofactor Protein; D008562:Membrane Glycoproteins; D000001:Calcimycin