The effect of clomiphene citrate and its Zu or En isomers on the reproductive system of the immature male rat.
The effect of clomiphene citrate (CC) and of its Zuclomiphene (ZuC) and Enclomiphene (EnC) isomers on the reproductive organs of immature male rats under different experimental conditions is reported. CC, ZuC, and EnC were administered daily to groups of either intact or castrated rats between the age of 21-44 d. This led to inhibition of weight increase of testis and accessory glands in the intact group. Spermatogenesis was arrested at the stage of primary spermatocyte following CC and ZuC treatment, and at the stage of young spermatids by EnC treatment. In the castrated group clomiphenes significantly stimulated weight increase of seminal vesicles (SV) compared with castrated control animals, but the former group were unable to achieve organ weight gain comparable to that in normal controls. Administration of human Chorionic Gonadotropin (hCG) together with CC or each of its isomers to intact animals, abolished the drug effect on spermatogenesis and on reproductive organ growth. Administration of CC, ZuC, and EnC together with testosterone to castrated animals, abolished the drug effect on growth inhibition of accessory glands. In intact treated rats LH and testosterone secretion were suppressed by all forms of clomiphenes. In the castrated group ZuC proved to be the most potent inhibitor of LH secretion. Therefore, it is inferred that ZuC and EnC have different potencies as far as their biological activity in the immature male rat is expressed.
Weissenberg
R
R
Institute of Endocrinology, Sheba Medical Center, Tel Hashomer, Israel.
Dar
Y
Y
Lunenfeld
B
B
D000818:Animals; D002996:Clomiphene / Q000737:chemistry / Q000494:pharmacology*; D064695:Enclomiphene; D005837:Genitalia, Male / Q000187:drug effects* / Q000254:growth & development / Q000502:physiology; D007986:Luteinizing Hormone / Q000378:metabolism; D008297:Male; D009919:Orchiectomy; D011467:Prostate / Q000187:drug effects / Q000254:growth & development / Q000502:physiology; D051381:Rats; D012669:Seminal Vesicles / Q000187:drug effects / Q000254:growth & development / Q000502:physiology; D012741:Sexual Maturation; D013091:Spermatogenesis / Q000187:drug effects; D013237:Stereoisomerism; D013737:Testis / Q000187:drug effects / Q000254:growth & development / Q000502:physiology; D013739:Testosterone / Q000378:metabolism / Q000494:pharmacology
D002996:Clomiphene; D013739:Testosterone; D007986:Luteinizing Hormone; D064695:Enclomiphene