Cytoprotective action of beraprost sodium against peroxide-induced damage in vascular endothelial cells.
Using a peroxide-injured endothelial cell model, beraprost sodium (beraprost) was tested in relation to the action to protect against the damage of vascular endothelial cells employing the viability of the cells and change in lipid peroxides as the indicators. At a concentration of 1-3 mumol/l or higher, beraprost significantly inhibited the decrease in viability of the cells and the increase in lipid peroxides level caused by t-butyl hydroperoxide or 15-hydroperoxy-5,8,11,13-eicosatetraenoic acid. When similar tests were conducted with prostaglandin I2, its inhibiting action was equal to or slightly weaker than that of beraprost unlike PGE1 and PGD2. This action of beraprost in inhibiting cell damage caused by peroxides suggests that beraprost may be useful for protecting cells from damage due to ischemic diseases.
Kainoh
M
M
Laboratory of Pharmacology, Toray Industries, Inc., Kanagawa, Japan.
Nishio
S
S
Nakadate
T
T
D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects*; D011464:Epoprostenol / Q000031:analogs & derivatives* / Q000494:pharmacology; D015289:Leukotrienes; D008054:Lipid Peroxides / Q000378:metabolism; D016877:Oxidants; D010545:Peroxides; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology*; D011465:Prostaglandins, Synthetic / Q000494:pharmacology; D014662:Vasoconstrictor Agents; D020122:tert-Butylhydroperoxide
D015289:Leukotrienes; D008054:Lipid Peroxides; D016877:Oxidants; D010545:Peroxides; D010975:Platelet Aggregation Inhibitors; D011465:Prostaglandins, Synthetic; D014662:Vasoconstrictor Agents; C048081:beraprost; C025086:15-hydroperoxy-5,8,11,13-eicosatetraenoic acid; D020122:tert-Butylhydroperoxide; D011464:Epoprostenol; D002118:Calcium