Simultaneous purification of the neuroproteins synapsin I and synaptophysin.
A procedure for the simultaneous purification of synapsin I and synaptophysin from calf brain was developed. Demyelinated membranes were extracted with 2% Triton X-100 and 2 M KCl. The extracted proteins were separated by weak cation-exchange chromatography on carboxymethyl-Sepharose Fast Flow. Synaptophysin was finally purified by preparative sodium dodecyl sulphate-polyacrylamine gel electrophoresis and synapsin I by affinity chromatography using a calmodulin-Sepharose column. The recovery obtained was 40 micrograms/g in brain for synaptophysin and 25 micrograms/g in brain for synapsin I.
Llona
I
I
Department of Medicine, University of Antwerp (UIA), Wilrijk, Belgium.
Annaert
W G
WG
De Potter
W P
WP
D000818:Animals; D001923:Brain Chemistry; D002417:Cattle; D002846:Chromatography, Affinity; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D017830:Octoxynol; D011092:Polyethylene Glycols; D011189:Potassium Chloride; D016704:Synapsins / Q000302:isolation & purification*; D016708:Synaptophysin / Q000302:isolation & purification*
D016704:Synapsins; D016708:Synaptophysin; D011092:Polyethylene Glycols; D011189:Potassium Chloride; D017830:Octoxynol