Differential expression of DNAseI-sensitive sites in metaphase chromosomes of T and B lymphocytes.
Thirty-one recurrent DNAseI-sensitive sites were expressed in G-banded metaphases of cultured T and B lymphocytes. Approximately one-half of these sites were found in both T and B cells and the other half were differentially expressed in the two cell types. The DNAseI-sensitive sites showed a strong correlation with the chromosomal gene location of T-cell receptors, interleukins produced by lymphocytes, and mapped surface antigens involved in the activation/proliferation of lymphocytes. The findings suggest that the chromatin of these very active genes remain accessible to DNAseI during cell division, and may help determine their precise chromosomal location.
Santos
J
J
Department of Neoplastic Diseases, Hahnemann University, Philadelphia, Pennsylvania.
Yunis
J J
JJ
D015703:Antigens, CD / Q000235:genetics; D001402:B-Lymphocytes / Q000378:metabolism*; D002877:Chromosomes, Human / Q000378:metabolism*; D003850:Deoxyribonuclease I / Q000378:metabolism*; D006801:Humans; D007378:Interleukins / Q000235:genetics; D008677:Metaphase / Q000235:genetics / Q000502:physiology; D011948:Receptors, Antigen, T-Cell / Q000235:genetics; D013601:T-Lymphocytes / Q000378:metabolism*
D015703:Antigens, CD; D007378:Interleukins; D011948:Receptors, Antigen, T-Cell; D003850:Deoxyribonuclease I