title: Parvovirus infection in children with AIDS: high prevalence of B19-specific immunoglobulin M and G antibodies.
abstract: OBJECTIVE
  Investigation of the prevalence and pathogenic role of parvovirus B19 infection in Italian and Rumanian children with AIDS, compared with age-matched HIV-negative children (controls) with various recurrent infections of unknown aetiology.
  
  
  METHODS
  Detection of B19-specific immunoglobulin (Ig) M and IgG antibodies as the most indicative markers of past or current B19 infection.
  
  
  METHODS
  B19 antibodies were detected by two enzyme immunoassays using synthetic peptide or recombinant protein, which corresponded to different B19 epitopes, as coating antigens.
  
  
  RESULTS
  B19 IgM and IgG were seen in 10 out of 20 (50%) Italian and in 20 out of 51 (39.2%) Rumanian children with AIDS, in contrast to none out of 17 Italian and one out of 22 Rumanian controls (P less than 0.001). In addition, two Italian controls (11.8%), two Rumanian children with AIDS (3.9%), and two Rumanian controls (9.1%) had B19 IgM alone. Specific IgG alone was detected in eight (40%) Italian and 14 (27.5%) Rumanian children with AIDS, and in seven (41.2%) Italian and four (10.2%) Rumanian controls.
  
  
  CONCLUSIONS
  While it is possible to attribute some B19 infections in Rumanian children to blood transfusion, the source was unknown for Italian children. However, in three of the Italian children who had B19 IgM and IgG persistently for 15-22 months, and in a 2-month-old Italian infant with B19 IgM and IgG, HIV might have activated a congenital or perinatally-acquired B19 infection.
authors.affiliation: Department of Pediatrics, La Sapienza University, Rome, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Nigro
authors.forename: G
authors.initials: G
authors.lastname: Luzi
authors.forename: E
authors.initials: E
authors.lastname: Fridell
authors.forename: M
authors.initials: M
authors.lastname: Ferrara
authors.forename: P
authors.initials: P
authors.lastname: Pisano
authors.forename: G C
authors.initials: GC
authors.lastname: Gattinara
authors.forename: I
authors.initials: I
authors.lastname: Mezzaroma
authors.forename: M
authors.initials: M
authors.lastname: Söderlund
authors.forename: D
authors.initials: D
authors.lastname: Rasnoveanu
authors.forename: F
authors.initials: F
authors.lastname: Aiuti
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000150:complications / Q000453:epidemiology / Q000276:immunology*; D000595:Amino Acid Sequence; D000914:Antibodies, Viral / Q000097:blood*; D000918:Antibody Specificity; D002675:Child, Preschool; D016731:Erythema Infectiosum / Q000453:epidemiology / Q000209:etiology / Q000276:immunology*; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood*; D007075:Immunoglobulin M / Q000097:blood*; D007223:Infant; D007558:Italy / Q000453:epidemiology; D008297:Male; D008969:Molecular Sequence Data; D016732:Parvovirus B19, Human / Q000276:immunology*; D011994:Recombinant Proteins / Q000276:immunology; D012383:Romania / Q000453:epidemiology
chemical_list: D000914:Antibodies, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D011994:Recombinant Proteins