Immunosuppressive molecules produced by Sertoli cells cultured in vitro: biological effects on lymphocytes.
In the present study we have analyzed the proteins secreted in vitro by murine Sertoli cells to identify immunosuppressive factors. Our data show that Sertoli cells secrete molecules capable to inhibit proliferation of lymphocytes activated in vitro. Cytophluorimetric analysis indicates that treated cells are arrested in the G1 phase of cell cycle. The inhibitory activity is specific for both B or T lymphocytes but not for other non-lymphoid cells and is associated to proteins, heat and freeze stable, with Mr of more than 30 kDa. Lymphocytes treated with Sertoli immunosuppressive proteins drastically reduce the secretion of interleukin-2.
De Cesaris
P
P
Institute of Histology & General Embryology, University of Rome La Sapienza, Italy.
Filippini
A
A
Cervelli
C
C
Riccioli
A
A
Muci
S
S
Starace
G
G
Stefanini
M
M
Ziparo
E
E
D000818:Animals; D001402:B-Lymphocytes / Q000187:drug effects / Q000276:immunology*; D002460:Cell Line; D002478:Cells, Cultured; D004261:DNA Replication; D005347:Fibroblasts / Q000276:immunology; D007166:Immunosuppressive Agents / Q000302:isolation & purification* / Q000494:pharmacology; D007376:Interleukin-2 / Q000096:biosynthesis; D007700:Kinetics; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009500:Neutralization Tests; D012708:Sertoli Cells / Q000276:immunology*; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology*; D013737:Testis / Q000276:immunology; D013936:Thymidine / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000276:immunology / Q000494:pharmacology*
D007166:Immunosuppressive Agents; D007376:Interleukin-2; D016212:Transforming Growth Factor beta; D013936:Thymidine