title: A selective inhibitor of cyclic AMP-dependent protein kinase, N-[2-bromocinnamyl(amino)ethyl]-5-isoquinolinesulfonamide (H-89), inhibits phosphatidylcholine biosynthesis in HeLa cells.
abstract: In this study, we report that the potent and selective inhibitor of cyclic AMP-dependent protein kinase, N-[2-bromocinnamyl(amino)ethyl]-5-isoquinolinesulfonamide (H-89) interferes with the incorporation of choline into phosphatidylcholine in HeLa cells. Treatment of cells with 10 microM H-89 for 1 h decreases the phosphatidylcholine biosynthesis by 50%. This inhibition is prevented by simultaneous addition of 10 microM forskolin, while the choline uptake itself is not affected by H-89.
authors.affiliation: Institut fur Molekularbiologie und Biochemie, Freien Universität Berlin, Germany.
authors.forename: C C
authors.initials: CC
authors.lastname: Geilen
authors.forename: M
authors.initials: M
authors.lastname: Wieprecht
authors.forename: T
authors.initials: T
authors.lastname: Wieder
authors.forename: W
authors.initials: W
authors.lastname: Reutter
mesh_terms: D005576:Colforsin / Q000494:pharmacology; D006367:HeLa Cells; D006801:Humans; D007546:Isoquinolines / Q000494:pharmacology*; D007700:Kinetics; D010713:Phosphatidylcholines / Q000096:biosynthesis*; D047428:Protein Kinase Inhibitors; D013449:Sulfonamides; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
chemical_list: D007546:Isoquinolines; D010713:Phosphatidylcholines; D047428:Protein Kinase Inhibitors; D013449:Sulfonamides; D005576:Colforsin; C063509:N-(2-(4-bromocinnamylamino)ethyl)-5-isoquinolinesulfonamide; D013755:Tetradecanoylphorbol Acetate