Ets-1 and Ets-2 protooncogene expression in theca cells of the adult mouse ovary.
We have investigated the mRNA expression of the Ets-1 and Ets-2 genes in murine gonads and found expression in adult ovaries. In situ hybridization experiments show that the Ets genes are predominantly expressed in theca cells and cells of ovarian interstitium. By gel retardation experiments we detected DNA binding proteins in ovaries that specifically bind to the ETS motif, suggesting the expression of Ets or Ets-related proteins. Our results raise the possibility of Ets-2 involvement in ovarian pathology seen in patients with Down's syndrome.
Rowe
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A
Ludwig Institute for Cancer Research, St. Mary's Hospital Medical School, London, United Kingdom.
Propst
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F
D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000302:isolation & purification; D005260:Female; D015870:Gene Expression; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D016376:Oligonucleotides, Antisense; D011505:Protein-Tyrosine Kinases / Q000096:biosynthesis* / Q000235:genetics; D050784:Proto-Oncogene Protein c-ets-1; D050787:Proto-Oncogene Protein c-ets-2; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis* / Q000235:genetics; D050783:Proto-Oncogene Proteins c-ets; D011519:Proto-Oncogenes; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D012097:Repressor Proteins; D013799:Theca Cells / Q000502:physiology*; D013950:Thymus Gland / Q000502:physiology; D015534:Trans-Activators; D014157:Transcription Factors
D004268:DNA-Binding Proteins; C107465:ERF protein, human; C494041:ETS1 protein, human; C494042:Ets1 protein, mouse; C494070:Ets2 protein, mouse; D009838:Oligodeoxyribonucleotides; D016376:Oligonucleotides, Antisense; D050784:Proto-Oncogene Protein c-ets-1; D050787:Proto-Oncogene Protein c-ets-2; D011518:Proto-Oncogene Proteins; D050783:Proto-Oncogene Proteins c-ets; D012333:RNA, Messenger; D012097:Repressor Proteins; D015534:Trans-Activators; D014157:Transcription Factors; D011505:Protein-Tyrosine Kinases