[Significance of adenylic acid catabolites for mouse thymus regeneration].
The distribution of [8-14C]adenylic acid catabolites in mouse thymocytes in cortisone-resistant and total thymocyte population has been studied. The accumulation of labelled catabolites in a form of hypoxanthine was found preferentially in cortisone resistant thymocytes but not in total population. This accumulation considerably grows after incubation of cortisone resistant thymocytes with non-peptide mitogenic factor. The excretion of labelled hypoxanthine into medium was also observed. In order to investigate a significance of hypoxanthine accumulation cortisone resistant thymocytes were incubated in the presence of hypoxanthine range concentration and [14C]thymidine incorporation into thymocyte DNA was determined. It was found that [14C]thymidine incorporation into thymocyte DNA increases after incubation in presence of 0.5-5.0 micrograms of hypoxanthine or 0.0005-5.0 micrograms of uric acid. It has been concluded that stimulation of [14C]thymidine incorporation and thymocyte proliferation by non-peptide mitogenic factor is caused by hypoxanthine accumulation.
Bezvershenko
I A
IA
Goĭdash
M M
MM
Boĭko
M G
MG
D000818:Animals; D003348:Cortisone / Q000494:pharmacology; D019271:Hypoxanthine; D007042:Hypoxanthines / Q000378:metabolism*; D051379:Mice; D012038:Regeneration; D013936:Thymidine / Q000378:metabolism; D013950:Thymus Gland / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*
D007042:Hypoxanthines; D019271:Hypoxanthine; D003348:Cortisone; D013936:Thymidine
Znachenna gipokantinu ta sechovoï kysloty dlia regeneratsiĭ tymusu myshi.