Comparative study of hemoglobin estimated by Drabkin's and Sahli's methods.
Hemoglobin was estimated by Sahli's and Drabkin's method in samples collected by finger prick and venepuncture. Statistical analysis revealed a significant difference in the hemoglobin values obtained by the two methods (P less than .01 & P less than .05 respectively). Sahli's method carried out by two different groups of workers in samples of blood collected by venepuncture showed statistically significant difference (P less than .001). This could be due to the inbuilt errors of Sahli's method including a subjective bias due to visual comparison. When same samples were subjected to Drabkin's method by the same group of workers no significant difference in the results were obtained (P less than .05). This is due to reliability of the Drabkins method. The aim of this comparative study of hemoglobin estimation by Sahli's and Drabkin's methods is to emphasize the sensitivity and reliability of Drabkin's over Sahli's and utility of Drabkin's method in undergraduate teaching schedule.
Balasubramaniam
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P
Department of Physiology, L.T.M.M. College, Sion, Bombay.
Malathi
A
A
D001800:Blood Specimen Collection; D006452:Hemoglobinometry / Q000379:methods*; D006801:Humans; D008706:Methemoglobin / Q000031:analogs & derivatives / Q000032:analysis; D012016:Reference Values
C007169:cyanomethemoglobin; D008706:Methemoglobin