title: Chemiluminescence detection of 8a-hydroperoxy-tocopherone in photooxidized alpha-tocopherol.
abstract: Photosensitized oxidation products of alpha-tocopherol were analyzed by reversed phase high-performance liquid chromatography (CL-HPLC) in combination with chemiluminescence detection, to measure hydroperoxide derivatives. An intense peak was observed on the chemiluminescence chromatogram, corresponding to 8a-hydroperoxy-alpha-tocopherone (alpha-tocopherol hydroperoxide), the identity of which was confirmed by 1H nuclear magnetic resonance and mass spectrometry. Observed peak intensities correlated with the peroxide value of oxidized alpha-tocopherol. As little as 50 pmol of 8a-hydroperoxy-alpha-tocopherone was detectable. The CL-HPLC method, which is specific for the detection of hydroperoxides, should prove useful in studies on tocopherol oxidation in foods and biological systems.
authors.affiliation: Department of Food Chemistry, Tohoku University, Sendai, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Miyazawa
authors.forename: T
authors.initials: T
authors.lastname: Yamashita
authors.forename: K
authors.initials: K
authors.lastname: Fujimoto
mesh_terms: D002851:Chromatography, High Pressure Liquid; D008163:Luminescent Measurements; D010084:Oxidation-Reduction; D014810:Vitamin E / Q000031:analogs & derivatives* / Q000032:analysis*; D024502:alpha-Tocopherol / Q000031:analogs & derivatives*
chemical_list: D014810:Vitamin E; C076563:8a-hydroperoxy-tocopherone; D024502:alpha-Tocopherol