title: Assessment of hepatic phagocytic activity by in vivo microscopy after liver transplantation in the rat.
abstract: Phagocytic activity of sinusoidal lining cells was studied in 32 livers of male Lewis rats by in vivo fluorescence microscopy with epiillumination. Normal livers (group 1, n = 8) were compared with orthotopic syngeneic liver grafts 90 min after reperfusion after a period of cold storage in University of Wisconsin solution for 17 hr (group 2, n = 10) or 24 hr (group 3, n = 14). After bolus injection of fluorescence-labeled latex particles (3 x 10(8)/kg; diameter = 1.1 micron), zonal distribution and kinetics of adherence of latex beads were quantified by off-line video analysis. Hepatocellular function was estimated by the rate of bile production and biliary concentrations of bile acids. In normal livers 50%, 37% and 13% of injected latex beads adhered in zones 1, 2 and 3, respectively, whereas in transplanted livers a more homogeneous distribution was found (group 2: 37%, 41%, 22%; group 3: 37%, 39%, 24%; p less than 0.01 vs. controls by analysis of variance). Kinetic analysis of phagocytic activity showed no significant difference between group 1 (88% adherence of visible latex beads 3 min after injection) and group 2 (90% adherence). However, after long-term preservation in group 3, sinusoidal adherence was significantly faster (96.4% adherence; p less than 0.001). Bile secretion in group 2 was lower than in controls and severely depressed in group 3 (group 1: 1.1 +/- 0.07 microliter/min/gm liver [mean +/- S.E.M.]; group 2: 0.8 +/- 0.07; group 3: 0.1 +/- 0.04; p less than 0.001) without significant changes in bile acid concentrations.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Institute for Surgical Research, University of Munich, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Post
authors.forename: A P
authors.initials: AP
authors.lastname: Gonzalez
authors.forename: P
authors.initials: P
authors.lastname: Palma
authors.forename: M
authors.initials: M
authors.lastname: Rentsch
authors.forename: A
authors.initials: A
authors.lastname: Stiehl
authors.forename: M D
authors.initials: MD
authors.lastname: Menger
mesh_terms: D000241:Adenosine; D000493:Allopurinol; D000818:Animals; D001646:Bile / Q000378:metabolism; D015925:Cryopreservation; D005978:Glutathione; D007328:Insulin; D008099:Liver / Q000378:metabolism / Q000502:physiology; D016031:Liver Transplantation / Q000502:physiology*; D008297:Male; D008856:Microscopy, Fluorescence; D009926:Organ Preservation / Q000009:adverse effects / Q000379:methods*; D019188:Organ Preservation Solutions; D010587:Phagocytosis / Q000502:physiology*; D011887:Raffinose; D051381:Rats; D011917:Rats, Inbred Lew; D012996:Solutions; D013997:Time Factors
chemical_list: D007328:Insulin; D019188:Organ Preservation Solutions; D012996:Solutions; C055206:University of Wisconsin-lactobionate solution; D000493:Allopurinol; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose