[The plasmid pEJ6.6 carrying the activated c-Ha-ras-1 oncogene increases the mutation frequency in Chinese hamster cells].
The induction of gene mutations and chromosome aberrations by the plasmid pEJ6.6 carrying the activated c-Ha-ras-1 oncogene from human bladder carcinoma was studied in cultured Chinese hamster cells. Both an increase in the frequency of gene mutations to 6-mercaptopurine resistance and of chromosome aberrations was observed after pEJ6.6 treatment as compared to control series (pBR322). Thus the results of experiments carried out show that the pEJ6.6 plasmid possesses a mutagenic activity.
Baron
E M
EM
Bobrysheva
I V
IV
Varshaver
N B
NB
D000818:Animals; D002460:Cell Line; D002869:Chromosome Aberrations / Q000235:genetics; D006224:Cricetinae; D003412:Cricetulus; D004351:Drug Resistance / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology*; D011905:Genes, ras / Q000235:genetics*; D006801:Humans; D015122:Mercaptopurine / Q000633:toxicity; D009154:Mutation / Q000235:genetics; D010957:Plasmids / Q000235:genetics*; D001749:Urinary Bladder Neoplasms / Q000235:genetics*
D015122:Mercaptopurine
Plazmida pEJ6.6, nesushchaia aktivirovannyĭ onkogen c-Ha-ras-1, povyshaet chastotu mutatsiĭ v kletkakh kitaĭskogo khomiachka.