Human immunodeficiency virus type 1 productive infection in staurosporine-blocked quiescent cells.
Staurosporine, an antibiotic known to inhibit cellular protein kinases, can reversibly block the progress of normal and tumour cells into the cell cycle. The ability of HIV-1 to infect and replicate in cells blocked by staurosporine was investigated. The results show that blocked, non-cycling cells can be productively infected by HIV-1, steadily releasing infectious progeny virus for several weeks. This suggests that at least in some cases, HIV-1 can be found in a stable and active state in resting, non-proliferating T cells.
Aranda-Anzaldo
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URA-CNRS, Laboratoire d'Immunobiologie, Faculté de Médecine, Paris, France.
Viza
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D000470:Alkaloids / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects*; D002460:Cell Line; D004247:DNA / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D015497:HIV-1 / Q000302:isolation & purification / Q000472:pathogenicity / Q000502:physiology*; D006801:Humans; D011493:Protein Kinase C / Q000037:antagonists & inhibitors*; D019311:Staurosporine; D013601:T-Lymphocytes / Q000166:cytology / Q000382:microbiology*; D013936:Thymidine / Q000378:metabolism; D014407:Tumor Cells, Cultured; D014779:Virus Replication
D000470:Alkaloids; D004247:DNA; D011493:Protein Kinase C; D019311:Staurosporine; D013936:Thymidine