title: Identification of metallothionein in cultured cells by immunoblotting and immunofluorescence using a new monoclonal antibody.
abstract: A hybridoma clone (MT45-5-3) producing an IgG-class monoclonal antibody specific for metallothioneins (MTs) was established. The monoclonal antibody (MT45) cross-reacted with mouse, rat and rabbit Cd(2+)-induced MTs 1 and 2 and Zn(2+)-induced MT 2 as assessed by enzyme-linked immunosorbent assay. When the antibody was used to detect the MTs transferred to nylon membranes after SDS-polyacrylamide gel electrophoresis, the antibody reacted with cultured human cell MTs as well as rat, mouse and rabbit MTs 1 and 2 even after carboxymethylation. The antibody could be used for the indirect immunofluorescence test for Cd(2+)-induced MTs in cultured human and mouse cells.
authors.affiliation: Department of Microbiology, Hoshi College of Pharmacy, Tokyo, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Kikuchi
authors.forename: M
authors.initials: M
authors.lastname: Irie
authors.forename: H
authors.initials: H
authors.lastname: Ikebuchi
authors.forename: J
authors.initials: J
authors.lastname: Sawada
authors.forename: T
authors.initials: T
authors.lastname: Terao
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D002478:Cells, Cultured; D003429:Cross Reactions; D005455:Fluorescent Antibody Technique; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D015151:Immunoblotting / Q000379:methods; D007074:Immunoglobulin G; D008668:Metallothionein / Q000032:analysis* / Q000276:immunology*; D051379:Mice; D011817:Rabbits; D051381:Rats; D013045:Species Specificity
chemical_list: D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D008668:Metallothionein