title: Automated sequencing and mapping of cosmid DNA with fluorescently-labeled dideoxynucleotide terminators.
abstract: We have established a method for directly sequencing cosmid DNA on an automated DNA sequencer. The major advantage of this method is that only small amounts of cosmid template DNA are needed for the sequencing reactions.
authors.affiliation: Laboratorium für Molekulare Biologie, LMU München, FRG.
authors.forename: B
authors.initials: B
authors.lastname: Obermaier
authors.forename: H
authors.initials: H
authors.lastname: Domdey
mesh_terms: D001483:Base Sequence; D003360:Cosmids / Q000235:genetics*; D004247:DNA; D005821:Genetic Techniques; D008969:Molecular Sequence Data; D009711:Nucleotides; D015183:Restriction Mapping; D013698:Templates, Genetic
chemical_list: D009711:Nucleotides; D004247:DNA