Clustering of C2-H2 zinc finger motif sequences within telomeric and fragile site regions of human chromosomes.
Ninety-three phage clones identified by hybridization with a C2-H2 zinc finger sequence probe have been grouped into 23 genetic loci. Partial sequencing verified that each locus belonged to the zinc finger family. Oligonucleotide primer pairs were developed from these sequences to serve as STS markers for these loci. One or more clones from each locus was mapped onto human metaphase chromosomes by fluorescence in situ hybridization. Several loci map to identical chromosomal regions, indicating the possible presence of multigene clusters. Zinc finger loci were found to reside predominantly either in telomeric regions or in chromosomal bands known to exhibit chromosome fragility. Chromosome 19 carries a disproportionate fraction (10 of 23) of the mapped zinc finger loci.
Lichter
P
P
Department of Genetics, Yale University School of Medicine, New Haven, Connecticut 06510.
Bray
P
P
Ried
T
T
Dawid
I B
IB
Ward
D C
DC
D000595:Amino Acid Sequence; D001483:Base Sequence; D043283:Chromosome Fragile Sites; D002873:Chromosome Fragility; D002874:Chromosome Mapping; D004247:DNA; D005455:Fluorescent Antibody Technique; D006801:Humans; D007621:Karyotyping; D008677:Metaphase; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D016335:Zinc Fingers / Q000235:genetics*
D004247:DNA