title: Assignment of the gene for human spasmolytic protein (hSP/SML1) to chromosome 21.
abstract: A human cDNA corresponding to the porcine pancreatic spasmolytic protein (PSP) was isolated, and the recombinant clone was originally termed hSP for human spasmolytic protein. Later, the term SML1 for spasmolysin was suggested for the human gene. This protein shows a remarkable sequence homology to pS2, a protein coded by an estrogen-induced gene isolated from the breast carcinoma cell line MCF-7. Although, at the DNA level, the gene sequences pS2 and hSP/SML1 display insufficient homology for cross-hybridization, their expression in tumor cells occurs with remarkable coordination. The human pS2 gene sequence has been assigned to chromosome 21, and we have therefore attempted to map the hSP/SML1 gene by using cDNA and Southern blotting of genomic DNAs from a panel of human-rodent somatic cell hybrids carrying different complements of human chromosomes. Interestingly, the hSP/SML1 gene is also localized on chromosome 21.
authors.affiliation: Institut für Humangenetik, Universität des Saarlandes, Homburg/Saar, Federal Republic of Germany.
authors.forename: B
authors.initials: B
authors.lastname: Theisinger
authors.forename: C
authors.initials: C
authors.lastname: Welter
authors.forename: K H
authors.initials: KH
authors.lastname: Grzeschik
authors.forename: N
authors.initials: N
authors.lastname: Blin
mesh_terms: D015139:Blotting, Southern; D002874:Chromosome Mapping; D002891:Chromosomes, Human, Pair 21 / Q000502:physiology*; D006801:Humans; D006822:Hybrid Cells; D036341:Intercellular Signaling Peptides and Proteins; D009077:Mucins; D009124:Muscle Proteins; D009479:Neuropeptides; D010455:Peptides / Q000235:genetics*; D000071164:Trefoil Factor-2; D000071165:Trefoil Factor-3
chemical_list: D036341:Intercellular Signaling Peptides and Proteins; D009077:Mucins; D009124:Muscle Proteins; D009479:Neuropeptides; D010455:Peptides; C000604007:TFF2 protein, human; C099037:TFF3 protein, rat; D000071164:Trefoil Factor-2; D000071165:Trefoil Factor-3; C034407:pancreatic spasmolytic polypeptide