title: Evidence for in vivo incorporation of D-lactate into peptidoglycan precursors of vancomycin-resistant enterococci.
abstract: The VanA ligase encoded by the vancomycin resistance plasmid pIP816 of Enterococcus faecium BM4147 condenses D-alanine with various D-2-hydroxy and D-2-amino acids in vitro. D-Lactate added to the culture medium restored the vancomycin resistance of a strain that does not produce the VanH dehydrogenase and therefore appears to be a substrate of VanA in vivo.
authors.affiliation: Unité des Agents Antibactériens, Institut Pasteur, Paris, France.
authors.forename: M
authors.initials: M
authors.lastname: Arthur
authors.forename: C
authors.initials: C
authors.lastname: Molinas
authors.forename: T D
authors.initials: TD
authors.lastname: Bugg
authors.forename: G D
authors.initials: GD
authors.lastname: Wright
authors.forename: C T
authors.initials: CT
authors.lastname: Walsh
authors.forename: P
authors.initials: P
authors.lastname: Courvalin
mesh_terms: D004352:Drug Resistance, Microbial; D016984:Enterococcus faecium / Q000187:drug effects / Q000378:metabolism*; D007773:Lactates / Q000378:metabolism*; D019344:Lactic Acid; D008826:Microbial Sensitivity Tests; D010457:Peptidoglycan / Q000378:metabolism*; D014640:Vancomycin / Q000378:metabolism / Q000494:pharmacology
chemical_list: D007773:Lactates; D010457:Peptidoglycan; D019344:Lactic Acid; D014640:Vancomycin