Subcellular fractionation evidence for a putative peroxisome-mitochondrion attachment in the liver of normal and genetically obese (ob/ob and db/db) mice.
1. Liver post-nuclear supernatants (PNS) from several mouse strains were fractionated by zonal centrifugation and fractions analysed by marker-enzyme estimations+electron microscopy. 2. Rate-dependent banding of PNS yielded peroxisome-enriched (PER) and mitochondrion-enriched (MER) regions. 3. Density-dependent banding of PER yielded peroxisomes (approximately 1.22 g/ml) well separated from mitochondria (approximately 1.8 g/ml). 4. Density-dependent banding of MER yielded peroxisomes that co-distributed with mitochondria and electron microscopy revealed close proximity of the two organelles. 5. Experiments demonstrated that co-distribution was not due to weak binding of proteins or to agglutination of organelles. 6. The results indicate in vivo attachment of some mitochondria and peroxisomes.
Mann
V M
VM
School of Applied Sciences, Wolverhampton Polytechnic, U.K.
Nwosu
V U
VU
Silcox
A
A
Jones
C J
CJ
Burdett
K
K
Connock
M J
MJ
D000818:Animals; D015415:Biomarkers; D002458:Cell Fractionation; D002499:Centrifugation, Density Gradient; D005260:Female; D008099:Liver / Q000201:enzymology / Q000648:ultrastructure; D008297:Male; D051379:Mice; D008820:Mice, Obese; D008830:Microbodies / Q000201:enzymology / Q000648:ultrastructure*; D008854:Microscopy, Electron; D008930:Mitochondria, Liver / Q000201:enzymology / Q000648:ultrastructure*
D015415:Biomarkers