[Quantitative analysis of DNA synthesis and cell condensation in the oligodactylic rat hand plate].
In order to clarify the teratogenic mechanism of oligodactyly which occurs during the hand plate development, pre-axial oligodactyly was induced in rat by myleran treatment on day 12.0 of gestation. This animal model was consecutively analyzed in the process of the hand plate development. DNA synthesis was quantified by a BrdU/ant-BrdU immuno histochemical study, and cell damage was assessed on the number of reactive macrophages. From these experiments, the following results were obtained. 1) In the oligodactylic rat embryos, the number of DNA-synthesizing cells was uniformly reduced in mesenchyme compared to that of the normal embryos on day 13.0. 2) In the pre-axial areas of the diseased hand plate, the number of DNA-synthesizing cells in ectoderm decreased significantly on day 13.5, and the reduction of DNA-synthesis was observed in all areas on day 14.0. 3) The damaged cells in the diseased hand plate were distributed uniformly, regardless of the lack of finger. Thus, it could be speculated that in this myleran induced oligodactylic model both the deficit of mesenchymal cells and the lack of ectoderm derived induction in the fingers were closely implicated in the development of oligodactyly.
Ohshio
I
I
Second Department of Pathology, Hokkaido University School of Medicine, Sapporo, Japan.
D000014:Abnormalities, Drug-Induced / Q000196:embryology* / Q000378:metabolism / Q000473:pathology; D000818:Animals; D001973:Bromodeoxyuridine / Q000032:analysis; D002066:Busulfan; D002452:Cell Count; D004247:DNA / Q000096:biosynthesis*; D005260:Female; D005528:Foot / Q000196:embryology*; D005552:Forelimb / Q000196:embryology; D005865:Gestational Age; D007150:Immunohistochemistry; D008264:Macrophages; D051381:Rats; D011919:Rats, Inbred Strains
D004247:DNA; D002066:Busulfan; D001973:Bromodeoxyuridine