Avid AL, a synthetic ligand affinity gel mimicking immobilized bacterial antibody receptor for purification of immunoglobulin G.
Avid AL is an affinity gel designed for the purification of immunoglobulin G (IgG). The gel was prepared by first reacting Sepharose with 3,5-dichloro-2,4,6-trifluoropyridine and 4-dimethylaminopyridine and then with 2-mercaptoethanol. The IgG purified by Avid AL is about 95% pure. The binding parameters of Avid AL for the whole IgG, Fab and Fc fragment and the stability of gel were investigated. The IgG bound to Avid AL can be eluted with an acidic buffer or with a novel neutral buffer containing electron donors. The development of such a mild neutral elution buffer is described. Application of Avid AL in a rapid gram-scale IgG purification was demonstrated. The possible mechanism of IgG binding is discussed.
Ngo
T T
TT
BioProbe International, Inc., Tustin, CA 92680.
Khatter
N
N
D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology / Q000302:isolation & purification*; D002021:Buffers; D002846:Chromatography, Affinity; D005782:Gels; D006041:Goats / Q000276:immunology; D006863:Hydrogen-Ion Concentration; D007074:Immunoglobulin G / Q000302:isolation & purification*; D008024:Ligands; D011725:Pyridines; D011946:Receptors, Antigen / Q000276:immunology / Q000302:isolation & purification*; D012685:Sepharose / Q000031:analogs & derivatives*
D000907:Antibodies, Bacterial; C076342:Avid AL gel; D002021:Buffers; D005782:Gels; D007074:Immunoglobulin G; D008024:Ligands; D011725:Pyridines; D011946:Receptors, Antigen; D012685:Sepharose