Determination of piroxicam and its major metabolites in the plasma, urine and bile of humans by high-performance liquid chromatography.
A simple and sensitive liquid chromatographic method with ultraviolet detection is described for the determination of the nonsteroidal anti-inflammatory drug piroxicam and its major metabolites in human plasma, urine and bile. Separation of these components occurs on a reversed phase C10CN column with a mobile phase consisting of acetonitrile-water-sodium dihydrogenphosphate solution. The detection limit of the assay was 50 ng/ml with intra- and inter-assay coefficients of variation for piroxicam of the order of 2 and 5%, respectively. The assay linearity was good (typically r = 0.9999). This method can be readily utilised for clinical pharmacokinetic and mass-balance studies.
Milligan
P A
PA
University of Glasgow, Department of Medicine and Therapeutics, Western Infirmary, Scotland, UK.
D000368:Aged; D001646:Bile / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006801:Humans; D007202:Indicators and Reagents; D010894:Piroxicam / Q000031:analogs & derivatives / Q000032:analysis* / Q000097:blood / Q000493:pharmacokinetics / Q000652:urine
D007202:Indicators and Reagents; D010894:Piroxicam; C051024:5'-hydroxypiroxicam