Use of antibody fragments in immunoaffinity chromatography. Comparison of FV fragments, VH fragments and paralog peptides.
Some new antibody fragments have recently been described: FV fragments (Mr 25,000), VH fragments or "dAbs" (12,500) and paralog peptides (1000-2000). FV fragments, VH fragments and a paralog peptide that had been derived from a parent antibody with a specificity for hen lysozyme were produced. All three reagents were immobilized on Sepharose and evaluated for their ability to recover hen lysozyme from "spiked" serum and to separate hen lysozyme from turkey lysozyme. The FV column had excellent specificity for hen lysozyme, the VH column had significantly reduced specificity and the paralog peptide column did not bind lysozyme at all.
Berry
M J
MJ
Immunology Department, Unilever Research, Sharnbrook, Bedfordshire, UK.
Davies
J
J
D000818:Animals; D002645:Chickens; D002846:Chromatography, Affinity; D003488:Cyanogen Bromide; D004591:Electrophoresis, Polyacrylamide Gel; D006736:Horses / Q000276:immunology; D007120:Immunochemistry; D007128:Immunoglobulin Fragments / Q000276:immunology*; D007163:Immunosorbent Techniques; D009113:Muramidase / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification; D010446:Peptide Fragments / Q000276:immunology*; D014422:Turkeys
D007128:Immunoglobulin Fragments; D010446:Peptide Fragments; D009113:Muramidase; D003488:Cyanogen Bromide