Cytogenetic applications of high resolution secondary ion imaging microanalysis: detection and mapping of tracer isotopes in human chromosomes.
Analytical imaging by secondary ion mass spectrometry (SIMS) using a state-of-the-art scanning ion microprobe enables the detection and mapping of tracer isotopes in human metaphase chromosomes. The stimulated mitosis of cells cultured in media containing labelled nucleosides, typically 14C-labelled thymidine or adenosine, and BrDU, yields chromosomes that have incorporated the labelled molecule in their constituent DNA. The label is subsequently detected and localized by SIMS imaging. The relative label signal intensities of sister chromatids can be quantified. The occurrence of sister chromatid exchanges (SCE) can be detected. The distribution of specific nucleosides can be directly mapped. This is non-uniform along the chromatids, giving rise to characteristic banding patterns (SIMS bands) that seem to correspond to the well known G- or Q-bands resulting from conventional staining methods. The study of a number of cytogenetic problems is expected to benefit from the use of this new method of approach, similar in principle, but potentially more sensitive and capable of higher spatial resolution than autoradiography.
Levi-Setti
R
R
Enrico Fermi Institute, Chicago, IL.
Le Beau
M
M
D001973:Bromodeoxyuridine; D002247:Carbon Isotopes; D002478:Cells, Cultured; D002877:Chromosomes, Human / Q000648:ultrastructure*; D006801:Humans; D007621:Karyotyping; D013058:Mass Spectrometry / Q000379:methods*; D008853:Microscopy / Q000379:methods*; D009705:Nucleosides / Q000032:analysis
D002247:Carbon Isotopes; D009705:Nucleosides; D001973:Bromodeoxyuridine