In vitro analysis of the cellular resistance to chemotherapeutic BCNU.
Our assays in vitro show that BCNU inhibits cell proliferation in the C6 cell line experimental glioma and is dose-dependent, starting from 0.5 microgram/ml of the drug with just an hour of exposure. For every tested concentration of BCNU it is shown that, from the fifth day after exposure, cellular resistance appeared. This resistance is justified by the capacity of cell DNA reparation. A study of the clonogenic capacity of the C6 cells exposed to BCNU also shows the appearance of cellular resistance for doses of 0.5 microgram/ml and 1 microgram/ml. Furthermore, the exposure of C6 cell cultures to BCNU at these levels produces a cellular evolution towards more differentiated morphological patterns.
Caballero Navarro
A
A
Department of Physiatry and Nursery, Faculty of Medicine, University of Zaragoza, Spain.
Conde Guerri
B
B
Sinues Porta
E
E
Boada Apilluelo
E
E
Alcalá Arellano
A
A
D002330:Carmustine / Q000008:administration & dosage / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002999:Clone Cells / Q000187:drug effects; D004260:DNA Repair / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004351:Drug Resistance; D005910:Glioma / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D006801:Humans; D014407:Tumor Cells, Cultured / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology
D002330:Carmustine