title: Measurement of markers of activated coagulation in antithrombin III deficient subjects.
abstract: Functional antithrombin III levels were measured by factor Xa inhibition in 63 members of a large family with type 2 antithrombin III deficiency and individuals were classified as antithrombin III deficient or non-deficient according to the results. F1 + 2 and TAT complexes were measured using an ELISA and FPA levels were measured by radioimmunoassay. Thirty subjects (48%) were classified as antithrombin III deficient and 33 (52%) as antithrombin III non-deficient. The mean level of F1 + 2 was significantly higher in the deficient adults (0.87 +/- 0.26) compared to both the non-deficient adults (0.70 +/- 0.21) (p = 0.03) and the deficient adults receiving warfarin (0.16 +/- 0.01) (p less than 0.001). The differences in the mean values of TAT complexes and FPA between deficient and non-deficient individuals were not statistically significant. These findings suggest that untreated antithrombin III deficient subjects generate more thrombin than their non-deficient family members and that warfarin inhibits this thrombin formation. In this cross-sectional study, it is not possible to correlate the levels of the surrogate makers with future clinical outcome.
authors.affiliation: Department of Medicine, McMaster University, Hamilton, Canada.
authors.forename: C
authors.initials: C
authors.lastname: Demers
authors.forename: J S
authors.initials: JS
authors.lastname: Ginsberg
authors.forename: P
authors.initials: P
authors.lastname: Henderson
authors.forename: F A
authors.initials: FA
authors.lastname: Ofosu
authors.forename: J I
authors.initials: JI
authors.lastname: Weitz
authors.forename: M A
authors.initials: MA
authors.lastname: Blajchman
mesh_terms: D000293:Adolescent; D000328:Adult; D000990:Antithrombin III / Q000378:metabolism*; D020152:Antithrombin III Deficiency; D015415:Biomarkers / Q000097:blood; D001777:Blood Coagulation / Q000502:physiology*; D002648:Child; D005344:Fibrinopeptide A / Q000378:metabolism*; D006801:Humans; D010446:Peptide Fragments / Q000378:metabolism*; D010447:Peptide Hydrolases / Q000378:metabolism*; D011516:Prothrombin / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D010446:Peptide Fragments; C046193:antithrombin III-protease complex; C045879:prothrombin fragment 1.2; D005344:Fibrinopeptide A; D000990:Antithrombin III; D011516:Prothrombin; D010447:Peptide Hydrolases