title: Preservation of hippocampal brain slices with in vivo or in vitro hypothermia.
abstract: Hippocampal brain slices show CA1 injury similar to that seen after global ischemia in vivo. Cooling rats to 31 degrees C prior to sacrifice or cooling slices to 21 degrees C for 45 min increased the percentage of normal CA1 pyramidal cells after 5 h in vitro from 30% to over 80%. Brain slices also show a unique, consistent injury in dentate which is lessened by transient cooling to 21 degrees C but not by cooling the animal.
authors.affiliation: Neurology Service, Veterans Administration Medical Center, Northport, NY 11768.
authors.forename: G C
authors.initials: GC
authors.lastname: Newman
authors.forename: H
authors.initials: H
authors.lastname: Qi
authors.forename: F E
authors.initials: FE
authors.lastname: Hospod
authors.forename: K
authors.initials: K
authors.lastname: Grundmann
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D015925:Cryopreservation / Q000379:methods*; D006624:Hippocampus / Q000473:pathology*; D007036:Hypothermia, Induced; D066298:In Vitro Techniques; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains