Modification by phenobarbital of decreased glutathione content and glutathione S-transferase activity in livers of lead-treated mice.
Lead acetate (100 mg/kg) administered i.p. to male mice decreased hepatic glutathione (GSH) content and also glutathione S-transferase (GST) activity. However, the liver GSH content of mice treated with both lead and phenobarbital (80 mg/kg, i.p.) remained unchanged, whereas their GST activities were higher than the controls. Phenobarbital antagonized the Pb-induced decrease in liver adenosine triphosphate content. Additionally, phenobarbital shortened the half-life of hepatic GSH determined using buthionine sulfoximine, an inhibitor of GSH synthesis. Acceleration of hepatic GSH turnover by phenobarbital possibly diminishes the Pb-induced impairment of GSH-conjugation of xenobiotics.
Nakagawa
K
K
Department of Food Science, Kyoto Women's University, Japan.
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D004347:Drug Interactions; D005978:Glutathione / Q000378:metabolism*; D005982:Glutathione Transferase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D008099:Liver / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009942:Organometallic Compounds / Q000633:toxicity*; D010634:Phenobarbital / Q000494:pharmacology*; D000623:Porphobilinogen Synthase / Q000187:drug effects / Q000378:metabolism; D013997:Time Factors; D015262:Xenobiotics / Q000378:metabolism
D009942:Organometallic Compounds; D015262:Xenobiotics; D000255:Adenosine Triphosphate; D005982:Glutathione Transferase; D000623:Porphobilinogen Synthase; D005978:Glutathione; C008261:lead acetate; D010634:Phenobarbital