title: Application of a microbial sensor for determination of short-chain fatty acids in raw milk samples.
abstract: A microbial sensor system, based on the use of immobilized Arthrobacter nicotiana and an oxygen electrode, was applied to determine free short-chain fatty acids in raw milk samples and the result was compared with gas chromatography (GC) and a titrimetric method. The sensor response was linearly related to the concentration of short-chain fatty acids obtained by GC (n = 10, r = 0.92) and to the total concentration of free fatty acids obtained by titrimetric measurement (n = 10, r = 0.78). This result suggests that the present microbial sensor can selectively determine free short-chain fatty acids in raw milk samples and may be useful as a very fast detection method of rancidity in milk.
authors.affiliation: GBF-Gesellschaft für Biotechnische Forschung, Department of Enzymtechnology, Braunschweig, Federal Republic of Germany.
authors.forename: H
authors.initials: H
authors.lastname: Ukeda
authors.forename: G
authors.initials: G
authors.lastname: Wagner
authors.forename: G
authors.initials: G
authors.lastname: Weis
authors.forename: M
authors.initials: M
authors.lastname: Miller
authors.forename: H
authors.initials: H
authors.lastname: Klostermeyer
authors.forename: R D
authors.initials: RD
authors.lastname: Schmid
mesh_terms: D000818:Animals; D001681:Biological Assay; D002417:Cattle; D002849:Chromatography, Gas; D005230:Fatty Acids, Nonesterified / Q000032:analysis; D005232:Fatty Acids, Volatile / Q000032:analysis; D008892:Milk / Q000032:analysis*
chemical_list: D005230:Fatty Acids, Nonesterified; D005232:Fatty Acids, Volatile