Gangliosides inhibit platelet-derived growth-factor-stimulated increases in intracellular calcium in Swiss 3T3 cells.
Individual Swiss 3T3 cells stimulated by platelet-derived growth factor delivered by means of a picopump device respond with a brisk, large, and sustained increase in intracellular calcium concentration ([Ca2+]i). Preincubation of cells with either GM1 or GT1b gangliosides inhibited the proportion of responding cells and caused a dose-related diminution in the magnitude of the increase in [Ca2+]i. This effect of ganglioside is probably part of the mechanism through which gangliosides exert their biological effects, including inhibition of platelet-derived growth-factor-induced mitogenesis.
Guan
Z
Z
Department of Physiology, College of Medicine, Ohio State University, Columbus.
Stokes
B T
BT
Van Brocklyn
J
J
Yates
A J
AJ
D016475:3T3 Cells / Q000187:drug effects; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D016257:Fura-2; D005677:G(M1) Ganglioside / Q000494:pharmacology*; D005732:Gangliosides / Q000494:pharmacology*; D051379:Mice; D010982:Platelet-Derived Growth Factor / Q000037:antagonists & inhibitors*
D005732:Gangliosides; D010982:Platelet-Derived Growth Factor; D005677:G(M1) Ganglioside; C016481:trisialoganglioside GT1; D002118:Calcium; D016257:Fura-2