title: Improvement of renal preservation by adding lidoflazine to University of Wisconsin solution. An experimental study in the rat.
abstract: The purpose of this study was to investigate the possibility of improving the organ preservation properties of the University of Wisconsin (UW) solution by adding the calcium entry blocker lidoflazine. We also investigated the possibility of decreasing the cold ischemia and reperfusion damage by pretreatment with lidoflazine of the donor and/or recipient. The protective effects of lidoflazine treatment were estimated by measuring the amount of trapped erythrocytes in the rat renal medulla after 48 h of cold storage, subsequent transplantation, and 20 min of reperfusion. Lidoflazine (20 mg/liter) added to the UW solution decreased the amount of erythrocyte trapping from 14.8 +/- 3.1% in controls to 8.6 +/- 1.7% (P less than 0.01). The flow rate of the flush-out solution during the harvesting procedure was also significantly (P less than 0.01) increased when lidoflazine was included in the UW solution (1.10 +/- 0.21 ml/min vs 0.75 +/- 0.22 ml/min). Administration of lidoflazine (0.28 mg/kg body wt) to the donor and/or the recipient did not further reduce the postischemia/reperfusion damage as estimated by the degree of erythrocyte trapping. In conclusion, the results indicate that the preservation properties of the UW solution can be significantly improved by adding lidoflazine to the solution.
authors.affiliation: Department of Urology, University Hospital, Uppsala, Sweden.
authors.forename: J
authors.initials: J
authors.lastname: Jacobsson
authors.forename: B
authors.initials: B
authors.lastname: Odlind
authors.forename: G
authors.initials: G
authors.lastname: Tufveson
authors.forename: J
authors.initials: J
authors.lastname: Wahlberg
mesh_terms: D000241:Adenosine; D000493:Allopurinol; D000818:Animals; D003080:Cold Temperature; D004912:Erythrocytes; D005978:Glutathione; D007328:Insulin; D007668:Kidney / Q000098:blood supply / Q000293:injuries; D016030:Kidney Transplantation; D008013:Lidoflazine; D008297:Male; D009926:Organ Preservation / Q000379:methods*; D019188:Organ Preservation Solutions; D011887:Raffinose; D051381:Rats; D011919:Rats, Inbred Strains; D015427:Reperfusion Injury / Q000517:prevention & control; D012996:Solutions
chemical_list: D007328:Insulin; D019188:Organ Preservation Solutions; D012996:Solutions; C055206:University of Wisconsin-lactobionate solution; D000493:Allopurinol; D005978:Glutathione; D008013:Lidoflazine; D000241:Adenosine; D011887:Raffinose