Synthesis of a large peptide mimicking the DNA binding properties of the sex determining protein, SRY.
The sex determining protein, SRY, has been recently described as containing a DNA binding motif, also called the SRY box. This 80 amino acid box was synthesized using the continuous flow solid-phase technique. The product was then purified and tested according to such diverse criteria as its intrinsic structure or its biological activity (DNA binding capacity), and compared to the full-length protein. The data indicate that the peptide is relevant for the properties described so far for the protein.
Poulat
F
F
Centre de Recherches de Biochimie Macromoléculaire, CNRS/INSERM, Montpellier, France.
Guichard
G
G
Gozé
C
C
Heitz
F
F
Calas
B
B
Berta
P
P
D000595:Amino Acid Sequence; D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid; D002942:Circular Dichroism; D004247:DNA / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism*; D008969:Molecular Sequence Data; D009687:Nuclear Proteins; D010455:Peptides / Q000138:chemical synthesis / Q000378:metabolism*; D051876:Sex-Determining Region Y Protein; D014157:Transcription Factors
D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D010455:Peptides; D051876:Sex-Determining Region Y Protein; D014157:Transcription Factors; D004247:DNA