Interleukin 1 pretreatment reduces the insulin response to glucose in chronic sucrose-fed rats.
The present studies were undertaken to determine whether interleukin 1 beta ([IL-1] 1.0 micrograms/kg, ip) pretreatment for 3 days impairs the adaptive response to sucrose feeding in rats. One week after the last IL-1 injection, when no differences in plasma glucose and serum immunoreactive insulin (IRI) levels were observed, sucrose feeding was started. Sucrose feeding for 4 weeks did not affect basal glucose levels, whereas basal IRI levels were increased in sucrose-fed rats without IL-1 pretreatment. Eight weeks later, plasma glucose levels were increased before and at 15 min after intravenous bolus of 0.5 g/kg of glucose in sucrose-fed rats with IL-1 pretreatment. Only in IL-1-treated sucrose-fed rats were basal and glucose-stimulated IRI levels significantly reduced, compared with those levels in sucrose-fed vehicle-treated rats. IL-1 decreased pancreatic IRI contents at 1 and 9 weeks after the injection. These data suggest that pancreatic damage by IL-1 attenuated insulin response to glucose stimulation after long-term sucrose feeding.
Shimizu
H
H
First Department of Internal Medicine, Gunma University School of Medicine, Maebashi, Japan.
Negishi
M
M
Uehara
Y
Y
Shimomura
Y
Y
Mori
M
M
Kobayashi
I
I
D000704:Analysis of Variance; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism*; D004040:Dietary Carbohydrates / Q000494:pharmacology*; D002149:Energy Intake; D007328:Insulin / Q000097:blood / Q000378:metabolism*; D000078790:Insulin Secretion; D007375:Interleukin-1 / Q000494:pharmacology*; D007515:Islets of Langerhans / Q000187:drug effects / Q000378:metabolism; D007700:Kinetics; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D011994:Recombinant Proteins / Q000494:pharmacology; D013395:Sucrose / Q000494:pharmacology*; D013997:Time Factors
D001786:Blood Glucose; D004040:Dietary Carbohydrates; D007328:Insulin; D007375:Interleukin-1; D011994:Recombinant Proteins; D013395:Sucrose