HPLC separation of 32P-postlabelled benzo[b]fluoranthene-DNA adducts.
Analysis using 32P-postlabelling and a recently developed HPLC method resolved the adduct formed by reaction of the benzo[b]fluoranthene (BbF) anti-bay-region diol-epoxide with DNA from the more polar major adduct produced by the hydrocarbon in three different biological systems. In each case, the adduct formed from the anti-bay-region diol-epoxide constituted only a minor proportion of the total DNA modification. Comparisons of the DNA adducts formed from the hydrocarbon with those formed in microsomal incubations from the putative metabolites BbF-9,10-diol, anti-BbF-9,10-diol-11,12-oxide and the 5,9,10- and 6,9,10-BbF-triols indicate that the predominant pathway for BbF activation in skin probably involves a bay-region triol-epoxide possessing a phenolic OH-group on the peninsula ring.
Pfau
W
W
Haddow Laboratory, Institute of Cancer Research, Sutton, Surrey, UK.
Hughes
N C
NC
Grover
P L
PL
Phillips
D H
DH
D000818:Animals; D001711:Biotransformation; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D005449:Fluorenes / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D006801:Humans; D051379:Mice; D008815:Mice, Inbred Strains; D008862:Microsomes, Liver / Q000378:metabolism*; D015394:Molecular Structure; D010761:Phosphorus Radioisotopes; D011865:Radioisotope Dilution Technique; D051381:Rats; D012867:Skin / Q000378:metabolism*
D005449:Fluorenes; D010761:Phosphorus Radioisotopes; D004247:DNA; C006703:benzo(b)fluoranthene