Detection of a transcriptional block in the first intron of the human c-myb gene.
The levels of expression of the murine c-myb gene, like those of several other proto-oncogenes, can be controlled by a block of transcriptional elongation within the first intron of the gene. We have performed run-off experiments with double- and single-stranded probes on the myelomonocytic cell line U937, and show that this mechanism of transcriptional arrest is true also for the human c-myb gene and takes place within the first intron. Furthermore, we have sequenced the entire first intron of the human c-myb gene, and discuss the sequence structure in relation to its putative ability to arrest RNA polymerase II and its high degree of homology with the equivalent murine intron.
Castellano
M
M
Istituto di Ricerche Farmacologiche Mario Negri, Milan, Italy.
Golay
J
J
Mantovani
A
A
Introna
M
M
D001483:Base Sequence; D002467:Cell Nucleus / Q000502:physiology; D016259:Genes, myc / Q000235:genetics*; D006801:Humans; D007438:Introns / Q000235:genetics*; D015477:Leukemia, Myelomonocytic, Chronic; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics; D014158:Transcription, Genetic / Q000235:genetics*; D014407:Tumor Cells, Cultured