Bordetella heat-labile toxin causes release of radioactivity from smooth muscle cells labeled with [14C]arachidonic acid.
The effect of Bordetella heat-labile toxin (HLT) on cultured vascular or tracheal smooth cells labeled with [14C]arachidonic acid for 3 h was examined. At 37 degrees C, in the presence of Ca2+, HLT induced the release of radioactive substances from the cells in a dose-dependent manner but HLT had no effect on release of radioactivity from cells at 0 degrees C or in the absence of Ca2+. After cells were exposed to HLT, a 2-h lag period occurred before release of radioactivity was detected. The substances released from cells by HLT were identified as arachidonic acid and phosphatidylcholine.
Nagai
M
M
Department of Microbiology, School of Pharmaceutical Sciences, Kitasato University, Tokyo, Japan.
Endoh
M
M
Burns
D L
DL
Nakase
Y
Y
D000818:Animals; D001011:Aorta; D016718:Arachidonic Acid / Q000378:metabolism*; D001884:Bordetella / Q000737:chemistry*; D002250:Carbon Radioisotopes; D005260:Female; D006168:Guinea Pigs; D009130:Muscle, Smooth / Q000187:drug effects* / Q000378:metabolism; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000378:metabolism; D013552:Swine; D014132:Trachea; D010566:Virulence Factors, Bordetella / Q000494:pharmacology*
D002250:Carbon Radioisotopes; D010566:Virulence Factors, Bordetella; D016718:Arachidonic Acid