Interleukin-1 beta stimulates growth of adrenocortical cells in primary culture.
Using tritiated-thymidine incorporation as a measure of cell growth, interleukin-1 beta stimulated the growth of bovine zona fasciculata/reticularis adrenocortical cells after 72 h in primary culture. Within the range of 10-1000 pg/ml, interleukin-1 beta produced over 40% of angiotensin II-stimulated [3H]thymidine incorporation (P less than 0.005 compared with basal for 10 pg/ml and 1000 pg/ml; P less than 0.05 for 100 pg/ml; two-tailed unpaired Student's t-test). Interleukin-1 beta did not directly stimulate cortisol secretion. By stimulating adrenocortical growth, the increase in interleukin-1 during fever provides a potential mechanism for chronically raising glucocorticoid output. This study is the first demonstration of a long-term effect involving interleukin-1 beta on the adrenal cortex.
Hanley
N
N
Department of Clinical Chemistry, Royal Infirmary, Edinburgh, U.K.
Williams
B C
BC
Nicol
M
M
Bird
I M
IM
Walker
S W
SW
D000818:Animals; D002417:Cattle; D002455:Cell Division / Q000502:physiology; D002478:Cells, Cultured; D006801:Humans; D006854:Hydrocortisone / Q000378:metabolism; D007375:Interleukin-1 / Q000502:physiology*; D011994:Recombinant Proteins; D013936:Thymidine; D015383:Zona Fasciculata / Q000166:cytology*; D015385:Zona Reticularis / Q000166:cytology*
D007375:Interleukin-1; D011994:Recombinant Proteins; D013936:Thymidine; D006854:Hydrocortisone