Influence on metastasis of the reduction of major histocompatibility complex (MHC) class I gene with an antisense oligonucleotide.
Cell surface MHC H-2K antigens expressed on various types of B16 melanoma cells of C57BL/6 mouse origin were stained by the avidin-biotinylated enzyme complex (ABC) technique. The low metastatic B16F1 cell line, which demonstrates high levels of H-2K antigen, stained strongly, while the high metastatic B16F10 cell line with no H-2K antigen was not stained. Metastatic colonies which developed in lungs after intravenous (i.v.) injection proved to be negative in both cases. Therefore, in consideration of decreased expression of H-2K antigen in the metastatic process both in vivo and in vitro, antisense oligonucleotides against the class I H-2K gene were transfected into B16F1 cells with H-2K antigens. Subsequent immunohistochemical assessment revealed lack of H-2K antigen expression in the cells, but nevertheless they still could not metastasize to lungs when i.v. injected. This may suggest that any link between the two is not directly causal.
Kanbe
M
M
Department of Clinical Cancer Chemotherapy, Tohoku University, Sendai, Japan.
Arita
D
D
Satoh
T
T
Yokoyama
A
A
Murakawa
Y
Y
Kikuchi
H
H
Kanamaru
R
R
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015870:Gene Expression / Q000235:genetics; D005805:Genes, MHC Class I / Q000235:genetics / Q000502:physiology*; D006183:H-2 Antigens / Q000032:analysis / Q000502:physiology; D015395:Histocompatibility Antigens Class I / Q000235:genetics / Q000502:physiology; D008175:Lung Neoplasms / Q000556:secondary; D008297:Male; D008546:Melanoma, Experimental / Q000235:genetics / Q000276:immunology / Q000556:secondary; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D009362:Neoplasm Metastasis / Q000235:genetics* / Q000276:immunology; D016376:Oligonucleotides, Antisense / Q000235:genetics / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics; D014162:Transfection
D006183:H-2 Antigens; C032876:H-2Kb protein, mouse; D015395:Histocompatibility Antigens Class I; D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger