title: A surface plasmon resonance immunosensor based on the streptavidin-biotin complex.
abstract: It is shown that a streptavidin monolayer immobilized onto an evaporated gold film with biotin forms the basis of a highly specific sensing element. As an example, we show that by immobilizing the biotinylated antibody sex hormone binding globulin (alpha-SHBG) to the bound streptavidin monolayer a specific sensor for the antigen SHBG is readily fabricated. The interaction between immobilized antibody and corresponding antigen is monitored by surface plasmon resonance spectroscopy and is shown to follow a classic Langmuir isotherm. Detection of SHBG at nanomolar concentrations is demonstrated.
authors.affiliation: Institute of Molecular and Biomolecular Electronics, University of Wales, Bangor, Gwynedd, UK.
authors.forename: H
authors.initials: H
authors.lastname: Morgan
authors.forename: D M
authors.initials: DM
authors.lastname: Taylor
mesh_terms: D000941:Antigens / Q000032:analysis; D001426:Bacterial Proteins; D015374:Biosensing Techniques; D001709:Biotechnology; D001710:Biotin; D005069:Evaluation Studies as Topic; D006801:Humans; D007118:Immunoassay / Q000379:methods; D012738:Sex Hormone-Binding Globulin / Q000032:analysis / Q000276:immunology; D013057:Spectrum Analysis; D019809:Streptavidin
chemical_list: D000941:Antigens; D001426:Bacterial Proteins; D012738:Sex Hormone-Binding Globulin; D001710:Biotin; D019809:Streptavidin