title: Immunohistochemical localization of inhibin/activin subunits in human placenta.
abstract: OBJECTIVE
  To examine the cellular localization of each inhibin subunit in human placenta throughout pregnancy.
  
  
  METHODS
  Placental tissues were collected and fixed in Bouin's solution, and studied with the immunohistochemical technique avidin-biotin-peroxidase complex.
  
  
  RESULTS
  There was immunohistochemical staining with antisera against each inhibin subunit in the syncytiotrophoblast, but not in the cytotrophoblast. In the first-trimester placenta, positive immunostaining for alpha- and beta A-subunits was clearly observed in the syncytial layer of villi, whereas staining for beta B-subunit was faint. In the second-trimester placenta, the relative intensities of staining for alpha- and beta A-subunits were similar to those in the first-trimester placenta, and enhanced positive immunostaining with beta B-subunit antiserum was observed. The relative amount of immunostainable alpha-subunit declined within the syncytiotrophoblast of the third-trimester placenta, whereas levels of immunostainable beta-subunits were unchanged.
  
  
  CONCLUSIONS
  Inhibin subunits may be produced in the syncytiotrophoblast throughout pregnancy, and activin as well as inhibin may be synthesized in the syncytiotrophoblast of the term placenta.
authors.affiliation: Department of Obstetrics and Gynecology, Wakayama Medical College, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Minami
authors.forename: M
authors.initials: M
authors.lastname: Yamoto
authors.forename: R
authors.initials: R
authors.lastname: Nakano
mesh_terms: D028341:Activins; D002824:Chorionic Villi / Q000737:chemistry; D005260:Female; D006133:Growth Substances / Q000032:analysis*; D006801:Humans; D007124:Immunoenzyme Techniques; D007265:Inhibins / Q000032:analysis*; D010920:Placenta / Q000737:chemistry*; D011247:Pregnancy
chemical_list: D006133:Growth Substances; D028341:Activins; D007265:Inhibins