Optimization of the sensitivity of high-performance liquid chromatography in the detection of heterocyclic aromatic amine mutagens.
Solid-phase extraction with the weak cation-exchange resin Fractogel TSK CM650(S) (TSK CM) was used to optimize the sensitivity and chromatographic resolution by HPLC of the mutagenic heterocyclic aromatic amines MeIQx, 4,8-DiMeIQx, IQ, MeIQ, PhIP, Trp-P-1, Trp-P-2, amino-alpha-carboline and the co-mutagens norharman and harman. The clean-up using cation exchange was applied after purification on Extrelut and propylsulphonic acid-silica gel cartridges (PRS tandem purification). Only the clean-up with TSK CM enabled low detection limits of the mutagenic compounds to be achieved (in the order of ng heterocyclic amines/g experimentally overheated process-flavour sample).
Gross
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GA
Nestec Ltd, Nestlé Research Centre, Vers-chez-les-Blanc, Lausanne, Switzerland.
Grüter
A
A
Heyland
S
S
D000588:Amines / Q000032:analysis* / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000592:standards*; D002852:Chromatography, Ion Exchange; D005504:Food Analysis / Q000379:methods*; D006358:Hot Temperature; D008461:Meat Products / Q000032:analysis*; D009153:Mutagens / Q000032:analysis* / Q000302:isolation & purification; D012680:Sensitivity and Specificity
D000588:Amines; D009153:Mutagens