title: Characterisation of a human bilirubin UDP-glucuronosyltransferase stably expressed in hamster lung fibroblast cell cultures.
abstract: A cDNA encoding a human bilirubin UDP-glucuronosyltransferase has been isolated and stably expressed in Chinese hamster V79 lung fibroblast cell line. Western blotting of cell homogenates with anti-UGT antibody revealed a highly expressed protein of approx. 55.5 kDa in size. The expressed enzyme specifically catalysed the formation of bilirubin mono- and diglucuronides, and also catalysed the glucuronidation of two phenolic compounds, which are good substrates for other human UGT isoenzymes, at low rates.
authors.affiliation: Department of Biochemical Medicine, University of Dundee, Ninewells Hospital and Medical School, Scotland, UK.
authors.forename: L
authors.initials: L
authors.lastname: Sutherland
authors.forename: S
authors.initials: S
authors.lastname: bin Senafi
authors.forename: T
authors.initials: T
authors.lastname: Ebner
authors.forename: D J
authors.initials: DJ
authors.lastname: Clarke
authors.forename: B
authors.initials: B
authors.lastname: Burchell
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D006224:Cricetinae; D003412:Cricetulus; D004247:DNA; D015870:Gene Expression; D014453:Glucuronosyltransferase; D006602:Hexosyltransferases / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism*; D008168:Lung / Q000166:cytology / Q000378:metabolism; D008862:Microsomes, Liver / Q000201:enzymology; D010957:Plasmids; D013379:Substrate Specificity
chemical_list: D007527:Isoenzymes; C019787:bilirubin glucuronoside glucuronosyltransferase; D004247:DNA; D006602:Hexosyltransferases; D014453:Glucuronosyltransferase