Differential retention of tumor- and differentiation-suppressor functions in cells derived from a human squamous cell carcinoma.
Three morphologically distinct cell lines--F.2a, V, and B.2--were isolated from a single human squamous cell carcinoma. Although all three cell lines can grow indefinitely in culture, they differ in a number of important transformation-related phenotypes. Only B.2 is strongly tumorigenic when injected into the flanks of nude mice, and only V can efficiently grow in semisolid media. The dominance of these traits was investigated by generating somatic cell hybrids among the three cell lines. F.2a was able to suppress the tumorigenicity of B.2 cells, whereas B.2 inhibited the capacity for anchorage-independent growth of V, the latter trait being a function of the ability of these epithelial cells to differentiate when deprived of support. The influence of exogenously added growth factors was also evaluated. This study indicates that the particular tumor we examined consisted of a heterogeneous population of cells with distinct growth and differentiation capacities.
Jaffe
D R
DR
Department of Radiation and Cellular Oncology, University of Chicago Medical Center, Illinois 60637.
Montero-Puerner
Y
Y
Beckett
M A
MA
Cowan
J M
JM
Weichselbaum
R R
RR
Diamond
A M
AM
D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000473:pathology*; D002448:Cell Adhesion; D002454:Cell Differentiation; D002455:Cell Division / Q000187:drug effects; D002459:Cell Fusion; D002460:Cell Line; D016895:Culture Media, Serum-Free; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D005819:Genetic Markers; D006801:Humans; D015603:Keratinocytes; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D010641:Phenotype; D014162:Transfection; D016211:Transforming Growth Factor alpha / Q000494:pharmacology*; D014183:Transplantation, Heterologous
D016895:Culture Media, Serum-Free; D005819:Genetic Markers; D016211:Transforming Growth Factor alpha; D004815:Epidermal Growth Factor