Identification of an acute-phase reactant in murine infections with Trypanosoma brucei.
A 42-kDa protein appeared at a much higher concentration in plasma from Trypanosoma brucei-resistant (C57BL/6) mice after infection than in plasma from trypanosome-susceptible (C3H/He) mice. This protein was purified by sequential steps of gel filtration, protein A-Sepharose affinity chromatography, isoelectric focusing, and ammonium sulfate precipitation. The purified protein was identified as a subunit of the acute-phase reactant haptoglobin. Causes of elevated plasma haptoglobin and its implications for resistance to trypanosomiasis are discussed.
Shapiro
S Z
SZ
International Laboratory for Research on Animal Diseases, Nairobi, Kenya.
Black
S J
SJ
D000818:Animals; D006242:Haptoglobins / Q000096:biosynthesis / Q000302:isolation & purification*; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008970:Molecular Weight; D013601:T-Lymphocytes / Q000276:immunology; D014346:Trypanosoma brucei brucei; D014353:Trypanosomiasis, African / Q000097:blood* / Q000276:immunology
D006242:Haptoglobins