Isolation of a new restriction enzyme, ApaCI, an isoschizomer of BamHI produced by Acetobacter pasteurianus.
A new Type II restriction endonuclease ApaCI purified from Acetobacter pasteurianus is an isoschizomer of BamHI that cleaves at the nucleotide sequence 5'-G/GATCC-3' of double-stranded DNA. The single restriction activity present in this strain permits rapidly purified 30,000 units of cleavage activity from 10 g of freshly harvested cells. The resulting ApaCI preparation is free of contaminant nuclease activities that might interfere with in vitro manipulation of DNA.
Grones
J
J
Department of Biochemistry, Faculty of Science, Comenius University, Bratislava, Czechoslovakia.
Turna
J
J
D000091:Acetobacter / Q000201:enzymology*; D010582:Bacteriophage lambda / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D001665:Binding Sites; D004279:DNA, Viral / Q000235:genetics / Q000378:metabolism; D015245:Deoxyribonuclease BamHI / Q000302:isolation & purification* / Q000378:metabolism; D013379:Substrate Specificity
D004279:DNA, Viral; D015245:Deoxyribonuclease BamHI