Matrix-assisted laser desorption mass spectrometry of proteins isolated by capillary zone electrophoresis.
A simple method for the off-line coupling of laser desorption mass spectrometry (LDMS) and capillary zone electrophoresis (CZE) is described. Representative mass spectra of subpicomole quantities of proteins isolated from CZE are presented and discussed. The current detection limit for bovine alpha-lactalbumin is 100 fmols injected onto the CZE column. Horse heart myoglobin was demonstrated to be stable in CHES/KCl, a CZE buffer, for at least 1 month, suggesting that some isolates can be safely stored for long time periods prior to LDMS analysis. Protein stability in 0.1% aqueous trifluoroacetic acid (TFA), a common solvent for LDMS, must also be considered. In the special case of porcine pepsinogen, significant (greater than 50%) degradation was observed within 5 min in TFA. In favorable cases, mass measurement accuracies of +/- 0.02% were obtained for protein isolates. Factors limiting mass measurement accuracy are presented. Finally, the possibility of identifying protein isolates, by combining N-terminal sequencing, molecular mass measurements, and selective peptide "mapping" procedures, is discussed.
Keough
T
T
Procter & Gamble Company, Miami Valley Laboratories, Cincinnati, Ohio 45239-8707.
Takigiku
R
R
Lacey
M P
MP
Purdon
M
M
D004586:Electrophoresis; D007834:Lasers; D013058:Mass Spectrometry; D011506:Proteins / Q000032:analysis* / Q000302:isolation & purification
D011506:Proteins