title: Further characterization of hormonal regulation of glutathione transferase in rat liver and adrenal glands. Sex differences and demonstration that growth hormone regulates the hepatic levels.
abstract: Immunoblot experiments and reverse-phase h.p.l.c. were used to study the levels of glutathione transferase subunits 1, 2, 3, 4, 6, 7 and 8 in the liver and adrenal of intact and hypophysectomized male and female Sprague-Dawley rats. A sexual dimorphism in the levels of several of these isoenzymes and in their responses to hypophysectomy was demonstrated. In the liver of sham-operated females and males there are differences in glutathione transferase activities and isoenzyme pattern. H.p.l.c. analysis showed higher levels of subunits 1, 3 and 4 in male rats compared with females. In contrast with the pronounced sex differences in sham-operated rats, the isoenzyme patterns of hypophysectomized males and females were very similar. In the adrenal glands, however, a sexual dimorphism became apparent only after hypophysectomy, when the level of subunit 4 was increased 14-fold in the female, whereas the corresponding increase in the male rat was only 2.7-fold. The hepatic pattern of glutathione transferase subunits could be altered by continuous infusion of growth hormone to both sham-operated and hypophysectomized rats of both sexes. This treatment feminized the isoenzyme pattern in sham-operated males and a similar effect was obtained upon treating hypophysectomized rats with thyroxine, cortisone acetate and a continuous infusion of growth hormone.
authors.affiliation: Department of Biochemistry, Wallenberg Laboratory, University of Stockholm, Sweden.
authors.forename: L
authors.initials: L
authors.lastname: Staffas
authors.forename: L
authors.initials: L
authors.lastname: Mankowitz
authors.forename: M
authors.initials: M
authors.lastname: Söderström
authors.forename: A
authors.initials: A
authors.lastname: Blanck
authors.forename: I
authors.initials: I
authors.lastname: Porsch-Hällström
authors.forename: C
authors.initials: C
authors.lastname: Sundberg
authors.forename: B
authors.initials: B
authors.lastname: Mannervik
authors.forename: B
authors.initials: B
authors.lastname: Olin
authors.forename: J
authors.initials: J
authors.lastname: Rydström
authors.forename: J W
authors.initials: JW
authors.lastname: DePierre
mesh_terms: D000311:Adrenal Glands / Q000187:drug effects / Q000201:enzymology*; D000818:Animals; D003348:Cortisone / Q000031:analogs & derivatives / Q000494:pharmacology; D003600:Cytosol / Q000201:enzymology; D005260:Female; D005982:Glutathione Transferase / Q000302:isolation & purification / Q000378:metabolism*; D013006:Growth Hormone / Q000494:pharmacology* / Q000502:physiology; D007016:Hypophysectomy; D007527:Isoenzymes / Q000302:isolation & purification / Q000378:metabolism*; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D046911:Macromolecular Substances; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012016:Reference Values; D012727:Sex Characteristics; D013267:Stilbenes / Q000378:metabolism; D013974:Thyroxine / Q000494:pharmacology; D013997:Time Factors
chemical_list: D007527:Isoenzymes; D046911:Macromolecular Substances; D013267:Stilbenes; D013006:Growth Hormone; D005982:Glutathione Transferase; C025906:stilbene oxide; D013974:Thyroxine; D003348:Cortisone