title: Process monitoring by flow-injection immunoassay. Evaluation of a sequential competitive binding assay.
abstract: A new variation on the theme of flow-injection binding assays is presented, namely the sequential competitive binding assay, in which the sample containing the native antigen is first introduced into the flow system followed by a pulse of labelled antigen. The flow-injection binding assay was used in monitoring the effluent from column chromatographic separations of proteins. By applying a computer-based evaluation system, concentrations are automatically read and compensation is made for denaturation in the affinity sorbent.
authors.affiliation: Department of Biotechnology, Lund University, Sweden.
authors.forename: M
authors.initials: M
authors.lastname: Nilsson
authors.forename: H
authors.initials: H
authors.lastname: Håkanson
authors.forename: B
authors.initials: B
authors.lastname: Mattiasson
mesh_terms: D001667:Binding, Competitive; D002850:Chromatography, Gel; D017022:Flow Injection Analysis / Q000379:methods*; D006735:Horseradish Peroxidase; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D007136:Immunoglobulins / Q000276:immunology; D011489:Protein Denaturation; D012709:Serum Albumin / Q000276:immunology
chemical_list: D007136:Immunoglobulins; D012709:Serum Albumin; D006735:Horseradish Peroxidase