Isolation of a phylogenetically conserved and testis-specific gene using a monoclonal antibody against the serological H-Y antigen.
Several cDNA clones of a gene termed male-enhanced antigen-2 (Mea-2), have been isolated from a mouse testicular expression cDNA library using a monoclonal histocompatability Y (H-Ys) antibody which detects specific protein(s) present in the mouse testis but not the ovary. The Mea-2 gene is phylogenetically conserved among various mammalian species examined, and is expressed at high levels in adult mouse testis. The expression pattern of Mea-2 is very similar to that of another gene, the male-enhanced antigen-1 (Mea-1), previously isolated using a polyclonal H-Ys antibody. Northern blotting and RT-PCR analyses demonstrated that Mea-2 is also expressed in other adult and fetal mouse organs at low levels. The testis-enhanced expression of this gene is associated with germ cell development at mid- to late-meiotic stages of spermatogenesis. Analysis of an intersubspecies mouse backcross has assigned this gene to chromosome 5, between the loci Gus and Hnf-1.
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Department of Medicine, University of California, San Francisco 94121.
Kozak
C A
CA
Veerhuis
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Lau
Y F
YF
Wiberg
U
U
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D001483:Base Sequence; D002874:Chromosome Mapping; D006224:Cricetinae; D003412:Cricetulus; D004247:DNA / Q000235:genetics; D005260:Female; D006168:Guinea Pigs; D006182:H-Y Antigen / Q000276:immunology*; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL / Q000235:genetics; D008969:Molecular Sequence Data; D010053:Ovary / Q000276:immunology; D010802:Phylogeny; D011506:Proteins / Q000235:genetics* / Q000276:immunology; D051381:Rats; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D013737:Testis / Q000737:chemistry / Q000276:immunology
D000911:Antibodies, Monoclonal; D006182:H-Y Antigen; D011506:Proteins; D004247:DNA