Ascaris suum--vaccination of mice with liposome encapsulated antigen.
Vaccination with liposome encapsulated adult crude antigen with and without coencapsulated immunomodulator (levamisole) in a mice/larval Ascaris suum model provided protection against a challenge infection (2000 eggs) in mice immunised by immobilised antigen. The best results (88.9% protection) were obtained with a combination of two doses of liposome entrapped antigen with leamisole. Vaccination with liposome vaccine without modulator was slightly less effective (78.7% protection). A single dose of vaccine was ineffective (14.3% protection). Application of the soluble antigen without any adjuvants led to the enhancement of worm yield in lungs and liver.
Lukes
S
S
Institute of Parasitology, CSAV, Ceské Budĕjovice, Czechoslovakia.
D000818:Animals; D000909:Antibodies, Helminth / Q000096:biosynthesis / Q000097:blood; D000947:Antigens, Helminth / Q000008:administration & dosage* / Q000276:immunology; D001196:Ascariasis / Q000517:prevention & control / Q000662:veterinary*; D001200:Ascaris / Q000276:immunology*; D004195:Disease Models, Animal; D004337:Drug Carriers; D005260:Female; D008081:Liposomes; D051379:Mice; D008813:Mice, Inbred ICR; D013552:Swine; D013553:Swine Diseases / Q000517:prevention & control*; D014611:Vaccination / Q000662:veterinary*
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D004337:Drug Carriers; D008081:Liposomes