Rapid isolation with Sep-Pak C18 cartridges and wide-bore capillary gas chromatography of bromisovalum.
A simple and rapid method for isolation of bromisovalum with Sep-Pak C18 cartridges from human whole blood, plasma, and urine containing allylisopropylacetylurea as an internal standard, is presented. Detection of the drugs was made by nonpolar wide-bore capillary gas chromatography (GC) with flame ionization. The drug-containing samples, after mixing with water, were directly applied to the cartridges and eluted with chloroform-methanol (9:1). The recoveries of bromisovalum with use of the cartridges were excellent. Bromisovalum and internal standard allylisopropylacetylurea could be satisfactorily separated from each other and from impurities with the wide-bore capillary column. The detection limit for bromisovalum was 2-5 ng in an injected volume.
Kumazawa
T
T
Department of Legal Medicine, Hamamatsu University School of Medicine, Japan.
Seno
H
H
Suzuki
O
O
D001968:Bromisovalum / Q000032:analysis* / Q000097:blood / Q000652:urine; D002849:Chromatography, Gas; D006801:Humans; D012015:Reference Standards; D014508:Urea / Q000031:analogs & derivatives
D001968:Bromisovalum; D014508:Urea; C005700:apronalide