Reversed-phase ion-pair high-performance liquid chromatographic determination of triclabendazole metabolites in serum and urine.
An ion-pair high-performance liquid chromatographic method was developed for measuring the concentrations of triclabendazole metabolites (sulphoxide and sulphone) in plasma and urine samples. The diluted biological fluids are ultrafiltered before chromatography through a 30,000 relative molecular mass cut-off filter and then injected into a C18 column. They are then isocratically eluted with a mobile phase consisting of 0.05 M phosphate buffer (pH 7.0)-acetonitrile (55:45, v/v) with addition of 1.0 mmol/l sodium decanesulphonate and monitored by ultraviolet-visible spectrophotometry at 312 nm. Recoveries over the range 0.01-9.0 micrograms/ml for triclabendazole sulphoxide and sulphone are, respectively, 91.7% and 91.6% in serum and 90.3% and 90.2% in urine. For both metabolites, the limit of detection is 10 ng/ml in both urine and serum.
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Departamento de Bioquímica y Biología Molecular, Universidad de León, Spain.
Alvarez-Bujidos
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ML
Ortiz
A I
AI
Cubría
J C
JC
Méndez
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R
Ordóñez
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D
D000818:Animals; D001562:Benzimidazoles / Q000032:analysis* / Q000097:blood / Q000652:urine; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002851:Chromatography, High Pressure Liquid; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D011817:Rabbits; D000077682:Triclabendazole; D014462:Ultrafiltration
D001562:Benzimidazoles; D007202:Indicators and Reagents; D000077682:Triclabendazole