Determination of manidipine enantiomers in human serum using chiral chromatography and column-switching liquid chromatography.
A stereoselective and highly sensitive method using chiral chromatography and successive column-switching liquid chromatography is described for the determination of manidipine enantiomers in human serum. A human serum sample obtained after ingestion of manidipine was extracted twice with a mixture of n-hexane-diethyl ether under alkaline conditions. The enantiomers in the extract were separated on a chiral stationary phase column (Chiralcel OJ), and the effluents containing the respective enantiomers were collected. Each fraction was then analysed by column-switching liquid chromatography. The proposed stereoselective method offered high sensitivity: detection limits for both isomers were 0.2 ng/ml in human serum, both at a signal-to-noise ratio of 3. The method is suitable for the pharmacokinetic studies of manidipine enantiomers.
Yamaguchi
M
M
Takeda Analytical Research Laboratories, Ltd., Osaka, Japan.
Yamashita
K
K
Aoki
I
I
Tabata
T
T
Hirai
S
S
Yashiki
T
T
D000284:Administration, Oral; D002121:Calcium Channel Blockers / Q000008:administration & dosage / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002853:Chromatography, Liquid / Q000379:methods*; D004095:Dihydropyridines / Q000008:administration & dosage / Q000097:blood*; D006801:Humans; D009578:Nitrobenzenes; D010879:Piperazines; D013237:Stereoisomerism
D002121:Calcium Channel Blockers; D004095:Dihydropyridines; D009578:Nitrobenzenes; D010879:Piperazines; C054218:manidipine