Ultrasonically induced in vitro cell lysis: node-antinode interactions.
An attempt was made to discriminate between two hypotheses (standing wave, bubble recycling) of the mechanism of ultrasonically induced cell lysis in a rotating tube. A tube containing an aqueous suspension of P-388 cells was moved back and forth (+/- 3 or +/- 7 mm) during insonation (1 MHz, 5 W/cm2, continuous wave, 5 min). Cell lysis (approximately 20%) occurred. As a positive control, some tubes were also partially or completely rotated during insonation; considerable cell lysis (approximately 60%) occurred. The results are interpreted to suggest that both hypotheses are simultaneously useful in explaining the observed effect of cell lysis in a rotating tube.
Doida
Y
Y
Department of Biology, Shiga University of Medical Science, Japan.
Brayman
A A
AA
Miller
M W
MW
D000388:Air; D000818:Animals; D016923:Cell Death; D002606:Charcoal; D003956:Dialysis / Q000295:instrumentation; D004867:Equipment Design; D007440:Intubation / Q000295:instrumentation; D051379:Mice; D009374:Neoplasms, Experimental / Q000473:pathology*; D011137:Polystyrenes; D012399:Rotation; D012408:Rubber; D014407:Tumor Cells, Cultured; D014465:Ultrasonics
D011137:Polystyrenes; D002606:Charcoal; C024075:styrofoam; D012408:Rubber