Variations of pre-proNPY mRNA in the arcuate nucleus during the rat estrous cycle.
In order to assess the role of sex steroids on the regulation of neuropeptide Y (NPY), levels of pre-proNPY mRNA were measured by in situ hybridization in the rat arcuate nucleus during the estrous cycle. Pre-proNPY mRNA levels were quite stable during diestrus I, diestrus II and the morning of the proestrus, and were 25-30% higher during the afternoon of the proestrus and during the day of the estrus. These data suggest that NPY synthesis is modulated by variations in the circulating levels of sex steroids. Also the excellent correlation between the variations in the levels of pre-proNPY mRNA and those of luteinizing hormone-releasing hormone (LHRH) mRNA observed throughout the estrous cycle supports the hypothesis that NPY might be involved in the regulation of LHRH secretion.
Pelletier
G
G
MRC Group in Molecular Endocrinology, CHUL Research Center, Ste-Foy, Quebec, Canada.
Rhéaume
E
E
Simard
J
J
D000818:Animals; D001111:Arcuate Nucleus of Hypothalamus / Q000378:metabolism*; D004971:Estrus / Q000378:metabolism*; D005260:Female; D015870:Gene Expression; D009478:Neuropeptide Y / Q000235:genetics / Q000378:metabolism*; D009693:Nucleic Acid Hybridization; D011498:Protein Precursors / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D009478:Neuropeptide Y; D011498:Protein Precursors; D012333:RNA, Messenger; C042084:preproneuropeptide Y