Development of a monoclonal antibody capable of differentiating platelet PLA1/PLA1, PLA1/PLA2 and PLA2/PLA2 genotypes.
A monoclonal antibody, LK-4, has been developed which distinguishes platelet PLA1/PLA1, PLA1/PLA2 and PLA2/PLA2 genotypes on platelet glycoprotein GPIIIa of Triton-solubilized platelet extracts. An ELISA assay has been developed which traps GPIIIa with Concanavalin A, enriching the platelet extract for the PLA antigens. A second monoclonal antibody, DEK-10, which reacts equally with GPIIIa of PLA1/PLA1 and PLA2/PLA2 platelet extracts is employed as an internal standard to correct for individual differences in GPIIIa content, GPIIIa extracted by Triton X-100 and GPIIIa trapped with Concanavalin A. This ELISA assay clearly differentiated 11 different PLA1/PLA1 subjects from eight PLA2/PLA2 women with a history of neonatal alloimmune thrombocytopenia as well as six unrelated obligate heterozygotes and should be useful in evaluating the PLA genotype of pregnant women and their families.
Liu
L X
LX
Department of Medicine, New York University Medical School, NY 10016.
Nardi
M
M
Flug
F
F
Karpatkin
S
S
D000483:Alleles; D000911:Antibodies, Monoclonal / Q000276:immunology*; D016824:Antigens, Human Platelet / Q000235:genetics* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005838:Genotype; D006579:Heterozygote; D006720:Homozygote; D006801:Humans; D015151:Immunoblotting; D010980:Platelet Membrane Glycoproteins / Q000235:genetics* / Q000276:immunology; D011247:Pregnancy; D011696:Purpura, Thrombocytopenic / Q000097:blood / Q000276:immunology
D000911:Antibodies, Monoclonal; D016824:Antigens, Human Platelet; D010980:Platelet Membrane Glycoproteins