Analysis of the T cell repertoire using the PCR and specific oligonucleotide primers.
Applications of PCR have revolutionized the field of immunogenetics particularly in studies of human leukocyte antigen class II polymorphism and more recently in the analysis of T cell receptor usage. The diversity of the variable region of the T cell receptor, however, has made it difficult to amplify the complete repertoire of T cell receptor transcripts. We have chosen to address this problem through the design of oligonucleotide primers specific for each of the known V alpha- and V beta-region T cell receptor families in order to characterize the T cell receptor repertoire. Using nonradioactive probes labeled with horse radish peroxidase, the system presented here allows for the rapid elucidation of the T cell receptor repertoire expressed in cells or tissue samples, such as those derived from autoimmune lesions. The identification of the T cell receptor repertoire involved in a pathogenic process can have therapeutic implications given the success of reversing experimental autoimmune disorders by directing specific forms of immunotherapy against V region gene products.
Panzara
M A
MA
Stanford University School of Medicine.
Gussoni
E
E
Steinman
L
L
Oksenberg
J R
JR
D000199:Actins / Q000235:genetics; D001483:Base Sequence; D015139:Blotting, Southern; D004247:DNA / Q000235:genetics; D015329:Gene Rearrangement, T-Lymphocyte; D006801:Humans; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D016133:Polymerase Chain Reaction / Q000379:methods*; D011948:Receptors, Antigen, T-Cell / Q000235:genetics / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*; D013698:Templates, Genetic
D000199:Actins; D009838:Oligodeoxyribonucleotides; D011948:Receptors, Antigen, T-Cell; D004247:DNA