title: Dependency on interleukin-1 of primary human in vitro T cell responses to soluble antigens.
abstract: The role of interleukin (IL)-1 in antigen-specific activation of naive human T cells has been examined. Primary human T cell proliferative responses to the soluble antigen keyhole limpet hemocyanin (KLH) were decreased by neutralizing antisera to IL-1 alpha (25 +/- 7% standard error) and IL-1 beta (56 +/- 6% standard error). Inhibition by both antisera in a primary culture was usually additive. Recombinant IL-1 alpha and recombinant IL-1 beta could both re-establish responses in cultures blocked by neutralizing anti-IL-1 beta. Interestingly, the susceptibility of KLH-stimulated T cell responses to inhibition by neutralizing anti-IL-1 sera decreased with time in culture. This observation suggested that T cell responses may become less IL-1 dependent as T cells become activated or primed. In support of this notion, secondary T cell responses to purified protein derivative from Mycobacterium tuberculosis (PPD) were markedly less affected by the addition of comparable amounts of the neutralizing anti-IL-1 sera. These results demonstrate that IL-1 is one of the main co-stimulators for primary T cell activation and suggest a different requirement for IL-1 in the activation of naive compared to memory human T cells.
authors.affiliation: Department of Pathology and Microbiology, School of Medical Sciences, University of Bristol, GB.
authors.forename: M
authors.initials: M
authors.lastname: Plebanski
authors.forename: C J
authors.initials: CJ
authors.lastname: Elson
authors.forename: W D
authors.initials: WD
authors.lastname: Billington
mesh_terms: D002478:Cells, Cultured; D006433:Hemocyanins / Q000276:immunology; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D007375:Interleukin-1 / Q000502:physiology*; D008213:Lymphocyte Activation; D011994:Recombinant Proteins / Q000494:pharmacology; D012995:Solubility; D013601:T-Lymphocytes / Q000276:immunology*; D013997:Time Factors; D014373:Tuberculin / Q000276:immunology
chemical_list: D007106:Immune Sera; D007375:Interleukin-1; D011994:Recombinant Proteins; D014373:Tuberculin; D006433:Hemocyanins; C032808:keyhole-limpet hemocyanin