Inhibition of ferrous-induced lipid peroxidation by pyrimido-pyrimidine derivatives in human liver membranes.
The effects of pyrimido-pyrimidine derivatives (dipyridamole, RA-642, and RA-233) on lipid peroxidation, using d-alpha-tocopherol as standard, were studied in enriched membrane fractions from human and rat hepatocytes. Equimolar concentrations of ferrous sulfate and ascorbic acid were used to induce lipid peroxidation. The amount of peroxidized lipids observed in membrane fractions from human liver was smaller than in those from rat liver. In both species, however, pyrimido-pyrimidine derivatives, except for RA-233 in rat liver, inhibited lipid peroxidation dose-dependently in the following sequence: RA-642 greater than dipyridamole greater than d-alpha-tocopherol RA-233.
de la Cruz
J P
JP
Department of Pharmacology and Therapeutics, School of Medicine, University of Málaga, Spain.
Carrasco
T
T
Ortega
G
G
Sanchez de la Cuesta
F
F
D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology; D002316:Cardiotonic Agents / Q000494:pharmacology; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism*; D004176:Dipyridamole / Q000494:pharmacology; D005296:Ferrous Compounds / Q000494:pharmacology*; D006801:Humans; D007700:Kinetics; D015227:Lipid Peroxidation / Q000187:drug effects*; D008099:Liver / Q000378:metabolism*; D008315:Malondialdehyde / Q000378:metabolism; D009012:Mopidamol / Q000494:pharmacology*; D011743:Pyrimidines / Q000494:pharmacology*; D051381:Rats; D013045:Species Specificity; D014810:Vitamin E / Q000494:pharmacology
D002316:Cardiotonic Agents; D005296:Ferrous Compounds; D011743:Pyrimidines; D014810:Vitamin E; C020748:ferrous sulfate; D009012:Mopidamol; D008315:Malondialdehyde; C010650:RA 642; D004176:Dipyridamole; D001205:Ascorbic Acid