title: A prospective controlled evaluation of TEST-yolk buffer in the preparation of sperm for human in vitro fertilization in suspected cases of male infertility.
abstract: OBJECTIVE
  To evaluate sperm preincubation in tes and tris (TEST)-yolk buffer as a potential enhancing agent of fertilizing capacity of sperm during human in vitro fertilization (IVF).
  
  
  METHODS
  Oocytes obtained during IVF were divided into two groups: one group fertilized with TEST-yolk buffer-treated sperm and the other group with standard-prepared sperm.
  
  
  METHODS
  The University of Southern California IVF Program.
  
  
  METHODS
  Thirty-nine couples with suspected male factor infertility undergoing IVF.
  
  
  METHODS
  Preinsemination incubation of sperm in TEST-yolk buffer for 24 hours.
  
  
  METHODS
  Fertilization rates in vitro.
  
  
  RESULTS
  TEST-yolk buffer-treated sperm fertilized 140 of 241 oocytes (58%), whereas control sperm fertilized 108 of 251 oocytes (43%). Of four couples who had previously failed to fertilize during IVF, one fertilized with both specimens and three fertilized with TEST-yolk buffer-treated sperm only.
  
  
  CONCLUSIONS
  TEST-yolk buffer pretreatment of sperm for 24 hours results in higher fertilization rates during IVF among suspected male factor patients.
authors.affiliation: Department of Obstetrics and Gynecology, University of Southern California School of Medicine, Los Angeles.
authors.forename: R J
authors.initials: RJ
authors.lastname: Paulson
authors.forename: M V
authors.initials: MV
authors.lastname: Sauer
authors.forename: M M
authors.initials: MM
authors.lastname: Francis
authors.forename: T M
authors.initials: TM
authors.lastname: Macaso
authors.forename: R A
authors.initials: RA
authors.lastname: Lobo
mesh_terms: D004530:Egg Yolk; D004624:Embryo Transfer; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D007248:Infertility, Male / Q000628:therapy*; D008297:Male; D011247:Pregnancy; D011446:Prospective Studies; D013094:Spermatozoa / Q000502:physiology*; D014325:Tromethamine
chemical_list: D014325:Tromethamine