Direct PCR of washed blood cells.
We report a simple and rapid method for direct DNA amplification of washed blood cells by PCR. Small samples (2-100 microliters) of blood were washed, the cells resuspended in a buffer and used directly for PCR after boiling. Amplification of a specific DNA sequence of the human transthyretin gene, directed by the primers, was successfully performed. The method gives comparable results to amplifications made by purified DNA from blood.
Nordvåg
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BY
Department of Rheumatology, Institute of Clinical Medicine, University Hospital of Tromsø, Norway.
Husby
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G
Raafat el-Gewely
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M
D000686:Amyloidosis / Q000235:genetics; D001773:Blood Cells; D004247:DNA / Q000032:analysis; D004587:Electrophoresis, Agar Gel; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*
D004247:DNA