title: Changes in protein synthesis pattern during in vitro maturation of goat oocytes.
abstract: The regulation of meiotic events of goat oocytes from prophase I to metaphase II was studied by inhibiting protein synthesis at different times of the transition and by analyzing the changes in the protein synthesis pattern during maturation. Protein synthesis was required for germinal vesicle breakdown (GVBD). Nevertheless, the concomitant event to the rupture of germinal vesicle, i.e., chromosome condensation, took place even in a cycloheximide-containing medium. The transition from metaphase I to metaphase II was also protein synthesis dependent as evidenced by experiments using this protein synthesis inhibitor. The inhibition was partly reversible, i.e., after removal of the drug, oocytes were able to progress until metaphase I but could not proceed beyond this stage. Changes in the protein synthesis pattern were studied by radiolabelling of oocytes with [35S]methionine. These changes were correlated with the nuclear status of the oocyte: At GVBD, a polypeptide of 25 kD disappeared, while one of 27 kD appeared. At the same time, a polypeptide of 33 kD appeared, whereas concomitantly one of 34 kD became barely detectable and finally disappeared as the maturation progressed. During maturation, the synthesis of a 67 kD polypeptide increased and became predominant at the end of the maturation process. The synthesis of actin decreased after 18 hr of culture from a very high to a low level of synthesis.
authors.affiliation: Unité de Biologie de la Fécondation, INRA, Jouy-en-Josas, France.
authors.forename: F
authors.initials: F
authors.lastname: Le Gal
authors.forename: L
authors.initials: L
authors.lastname: Gall
authors.forename: V
authors.initials: V
authors.lastname: De Smedt
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000648:ultrastructure; D003513:Cycloheximide / Q000494:pharmacology; D004527:Egg Proteins / Q000096:biosynthesis*; D005260:Female; D006041:Goats / Q000378:metabolism*; D008540:Meiosis; D009865:Oocytes / Q000378:metabolism* / Q000648:ultrastructure; D009866:Oogenesis
chemical_list: D004527:Egg Proteins; D003513:Cycloheximide