title: Amphiphilic behavior of a brain tetrameric acetylcholinesterase form lacking the plasma membrane anchoring domain.
abstract: We have studied the behavior of a mammalian brain tetrameric acetylcholinesterase (AChE) form released by proteinase K from a crude membrane fraction of bovine caudate nucleus. The solubilization of active AChE indicated the presence of a protease-sensitive site in the anchored protein. Unexpectedly, the solubilized AChE maintained its capacity to form aggregates in detergent-free gradients. We demonstrate here that this property was due neither to the presence of the hydrophobic membrane-anchoring domain still linked to the enzyme, nor to the presence of AChE activity trapped in small plasma membrane vesicles. Moreover, we found that the proteinase K-treated extract, devoid of AChE activity, induced the aggregation of purified hydrophilic AChE which usually does not form aggregates. Our results suggest the presence of an AChE aggregating factor in bovine brain extracts prepared in the presence of proteinase K. It is possible that this aggregation may reflect a process of AChE clustering on neurons.
authors.affiliation: Molecular Neurobiology Unit, Faculty of Biological Sciences, Catholic University of Chile, Santiago.
authors.forename: M E
authors.initials: ME
authors.lastname: Fuentes
authors.forename: N C
authors.initials: NC
authors.lastname: Inestrosa
mesh_terms: D000110:Acetylcholinesterase / Q000737:chemistry*; D000818:Animals; D001704:Biopolymers; D001921:Brain / Q000201:enzymology*; D002417:Cattle; D002421:Caudate Nucleus / Q000201:enzymology*; D002462:Cell Membrane / Q000502:physiology; D002846:Chromatography, Affinity; D003902:Detergents; D019286:Endopeptidase K; D046911:Macromolecular Substances; D017830:Octoxynol; D011092:Polyethylene Glycols; D012697:Serine Endopeptidases / Q000737:chemistry*; D012995:Solubility; D014867:Water / Q000737:chemistry
chemical_list: D001704:Biopolymers; D003902:Detergents; D046911:Macromolecular Substances; D014867:Water; D011092:Polyethylene Glycols; D017830:Octoxynol; C010615:Nonidet P-40; D000110:Acetylcholinesterase; D012697:Serine Endopeptidases; D019286:Endopeptidase K