Increased efficiency of gene transfection in primary cultures of adult rat hepatocytes stimulated to proliferate: a comparative study using the lipofection and the calcium phosphate precipitate methods.
Transfection of the beta-galactosidase gene in quiescent cultures of adult rat hepatocytes with the calcium phosphate precipitate or the lipofection methods gave a higher level of beta-galactosidase gene expression with the lipofection than with the calcium phosphate precipitate method, but the transfection efficiency was weak in both cases. Transfection of hepatocytes stimulated to proliferate before transfection either in vivo by partial hepatectomy or in vitro by epidermal growth factor was more efficient than transfection of quiescent hepatocytes, and the lipofection method gave better results than the calcium phosphate precipitate method.
Somasundaram
C
C
Laboratoire de Biologie Cellulaire, INSERM U 327, Faculté de Médecine X. Bichat, Paris, France.
Tournier
I
I
Feldmann
G
G
Bernuau
D
D
D000818:Animals; D002130:Calcium Phosphates; D002455:Cell Division; D002478:Cells, Cultured; D011232:Chemical Precipitation; D004815:Epidermal Growth Factor / Q000494:pharmacology; D005069:Evaluation Studies as Topic; D005821:Genetic Techniques; D008081:Liposomes; D008099:Liver / Q000166:cytology / Q000201:enzymology*; D051381:Rats; D014162:Transfection / Q000187:drug effects; D001616:beta-Galactosidase / Q000235:genetics*
D002130:Calcium Phosphates; D008081:Liposomes; C485828:alpha-tricalcium phosphate; C485830:tetracalcium phosphate; D004815:Epidermal Growth Factor; C485838:calcium phosphate, monobasic, anhydrous; C020243:calcium phosphate; D001616:beta-Galactosidase; C485829:calcium phosphate, dibasic, anhydrous