TCP enhances the survival of human fetal spinal cord cells in culture.
Dissociated cell cultures were prepared from human spinal cords of 7-10-week-old fetuses. After 10 weeks progressive neuronal necrosis was observed in controls whereas N/1-(2-thienyl)cyclohexyl/piperidine (TCP) enhanced the survival time of the cells. After 21 weeks the number of gamma-aminobutyric acid (GABA)ergic and neuron specific enolase (NSE)-stained neurons was higher in the TCP-treated cultures than in controls. TCP appears to be a promising drug for long term survival of neurons.
Levallois
C
C
INSERM U336, Ecole Nationale Supérieure de Chimie, Montpellier, France.
Calvet
M C
MC
Kamenka
J M
JM
Petite
D
D
Privat
A
A
D015415:Biomarkers; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002795:Choline O-Acetyltransferase / Q000378:metabolism; D004622:Embryo, Mammalian; D005333:Fetus; D006801:Humans; D009457:Neuroglia / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D009474:Neurons / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D010622:Phencyclidine / Q000031:analogs & derivatives* / Q000494:pharmacology; D010751:Phosphopyruvate Hydratase / Q000378:metabolism; D013116:Spinal Cord / Q000166:cytology* / Q000187:drug effects; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
D015415:Biomarkers; D005680:gamma-Aminobutyric Acid; C012058:tenocyclidine; D002795:Choline O-Acetyltransferase; D010751:Phosphopyruvate Hydratase; D010622:Phencyclidine