[An ellipsometric study of the antigen-antibody interaction in the interphase solid/solution area].
Ellipsometric studies have proved that monoclonal immunoglobulin G(IgG) against gamma-interferon (gamma-INF) and immunoglobulin fraction (Ig-fraction) of rabbit blood serum against human serum albumin (HSA) are adsorbed according to the Langmuir model on the surfaces of mirror plates of covalently modified gamma-INF or HSA, respectively. The maximum surface concentrations (Tmax) and equilibrium adsorption constants (K) for IgG and Ig-fraction are equal to 2.57 pmol/cm2 and 2 x 10(7) M-1, 3.3 mg/m2 and 0.1 cm3/micrograms, respectively. The additional treatment of gamma-INF modified surfaces with Tween-20 leads to an increase of K IgG ut to 2.7 x 10(-7) M-1 while Tmax decreases up to 1.12 pmol/cm2 which is conditioned by the blocking of protein non-specific binding sites. The role of specific and non-specific interactions of IgG and Ig-fraction with covalently immobilized antigens was studied at antibody-antigen mixture adsorption. The necessity to apply this method to quantitative determination of gamma-IHF and HSA in solutions was proved.
Ruzgas
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Razumas
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Kulis
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D000911:Antibodies, Monoclonal / Q000276:immunology; D000937:Antigen-Antibody Reactions; D007136:Immunoglobulins / Q000276:immunology; D007371:Interferon-gamma / Q000276:immunology; D012709:Serum Albumin / Q000276:immunology; D012996:Solutions
D000911:Antibodies, Monoclonal; D007136:Immunoglobulins; D012709:Serum Albumin; D012996:Solutions; D007371:Interferon-gamma
Ellipsometricheskoe izuchenie vzaimodeĭstviĭ antigen-antitelo v mezhfaznoĭ oblasti tverdoe telo/rastvor.