title: Use of various types of column reactors for flow-injection analysis.
abstract: Two or three different kinds of immobilized enzymes can be aligned in a minireactor so that sequential enzymatic reactions are carried out from upstream to downstream during flow-injection analysis. A lactate oxidase-catalase reactor, used as precolumn for removing pre-existing lactate in serum before the lactose dehydrogenase (LDH) reactions, was useful for the determination of serum LDH activity, which did not require any blank correction. A sequential glutamate dehydrogenase-glutamate oxidase reactor was also useful for a novel chemiluminometric determination of ammonia. On the other hand, a co-immobilized creatininase-creatinase-sarcosine oxidase reactor, in spite of containing creatininase which catalyses the reversible reaction, was the most efficient for the determination of serum creatinine.
authors.affiliation: College of Medical Technology, Kyoto University, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Tabata
authors.forename: T
authors.initials: T
authors.lastname: Murachi
authors.forename: J
authors.initials: J
authors.lastname: Endo
authors.forename: M
authors.initials: M
authors.lastname: Totani
mesh_terms: D000641:Ammonia / Q000032:analysis; D001786:Blood Glucose / Q000032:analysis; D003404:Creatinine / Q000097:blood; D004800:Enzymes, Immobilized; D017022:Flow Injection Analysis / Q000295:instrumentation*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D007770:L-Lactate Dehydrogenase / Q000097:blood
chemical_list: D001786:Blood Glucose; D004800:Enzymes, Immobilized; D007202:Indicators and Reagents; D000641:Ammonia; D003404:Creatinine; D007770:L-Lactate Dehydrogenase