[Integration of the plasmid-cloned lysA gene of Bacillus subtilis into the chromosome of this microorganism].
Integration of the Bacillus subtilis lysA gene cloned on pLP1 plasmid, into the 250 degrees region of the chromosome of this microorganism was performed. Significant differences in the level and character of their expression were shown between lysA gene integrated into the chromosome and plasmid-borne genes. It is suggested that the expression of the lysA gene could be under control of a certain cis-acting factor which is able to promote transcription of the lysA gene and mediate gene specific regulation.
Kal'cheva
E O
EO
Shanskaia
V O
VO
Maliuta
S S
SS
D001412:Bacillus subtilis / Q000235:genetics*; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial / Q000235:genetics*; D005818:Genetic Engineering; D005819:Genetic Markers / Q000235:genetics; D010957:Plasmids / Q000235:genetics*
D004269:DNA, Bacterial; D005819:Genetic Markers
Integratsiia klonirovannogo na plazmide lysA-gena Bacillus subtilis v khromosomy étogo mikroorganizma.