title: Detection of Chlamydia trachomatis by use of polymerase chain reaction.
abstract: A polymerase chain reaction (PCR) assay was developed for detection of Chlamydia trachomatis DNA. One primer set was used, from the published sequence of the common C. trachomatis plasmid. Detection of amplified sequences was carried out by agarose gel electrophoresis. Analysis of 106 clinical samples tested by cell culture and PCR showed a sensitivity of 100% when PCR was compared with cell culture.
authors.affiliation: Laboratoire d'Anthropologie Physique, Collège de France, Paris.
authors.forename: G
authors.initials: G
authors.lastname: Lucotte
authors.forename: M C
authors.initials: MC
authors.lastname: Petit
authors.forename: M H
authors.initials: MH
authors.lastname: François
authors.forename: S
authors.initials: S
authors.lastname: Réveilleau
mesh_terms: D001483:Base Sequence; D002478:Cells, Cultured; D002584:Cervix Uteri / Q000382:microbiology; D002690:Chlamydia Infections / Q000175:diagnosis; D002692:Chlamydia trachomatis / Q000235:genetics / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000032:analysis; D005260:Female; D006801:Humans; D008969:Molecular Sequence Data; D009841:Oligonucleotides; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction
chemical_list: D004269:DNA, Bacterial; D009841:Oligonucleotides