Cryoprotective effects of yeast extracellular polysaccharides and glycoproteins.
Eighteen yeast strains were tested for their ability to survive the freeze-thaw process while being cryoprotected. Cryoprotection was accomplished by combining penetrating and nonpenetrating cryoagents. Four nonpenetrating (two extracellular polysaccharides of yeast and two extracellular glycoproteins of yeast) and two penetrating agents were used together with the nutritive-rich medium. Eight different mixtures were tested. The highest survival rate was obtained with glycoproteins of Rhodosporidium toruloides together with DMSO and nutritive-rich medium.
Breierová
E
E
Czechoslovak Collection of Yeasts, Slovak Academy of Sciences, Bratislava.
Kocková-Kratochvílová
A
A
D000596:Amino Acids / Q000032:analysis; D002241:Carbohydrates / Q000032:analysis; D015925:Cryopreservation / Q000379:methods; D003451:Cryoprotective Agents / Q000737:chemistry / Q000494:pharmacology*; D004121:Dimethyl Sulfoxide; D005656:Fungal Proteins / Q000737:chemistry / Q000494:pharmacology; D006023:Glycoproteins / Q000737:chemistry / Q000494:pharmacology*; D011134:Polysaccharides / Q000737:chemistry / Q000494:pharmacology*; D015003:Yeasts
D000596:Amino Acids; D002241:Carbohydrates; D003451:Cryoprotective Agents; D005656:Fungal Proteins; D006023:Glycoproteins; D011134:Polysaccharides; D004121:Dimethyl Sulfoxide