title: Effects of 2,4-dinitrophenol or low [ATP]i on cell excitability and action potential propagation in guinea pig ventricular myocytes.
abstract: Inhibition of aerobic metabolism leads to a major disruption of cardiac cell homeostasis. The purpose of the present study was twofold: 1) We determined the relative importance of junctional and nonjunctional membrane resistance (Rj and Rm, respectively) in the development of propagation failure during inhibition of aerobic metabolism in guinea pig ventricular cell pairs. 2) We used the patch-action potential clamp technique in single ventricular myocytes to study some of the properties of the membrane channels that are responsible for shortening of action potential duration and eventual failure of cell excitation after metabolic blockade. In most experiments, whole-cell patch pipettes were filled with a solution containing 1 mM EGTA, 5 mM HEPES, and 5 mM ATP. Our results in cell pairs showed that pharmacological inhibition of aerobic metabolism with the mitochondrial uncoupler 2,4-dinitrophenol (DNP) led to a drop in Rm followed by an increase in Rj. The increase in Rj was not sufficient to cause a measurable delay in cell-to-cell propagation, whereas the drop in Rm consistently led to failure of cell excitation. Similar results were obtained in additional experiments in which the EGTA concentration in the pipette was reduced to 50 microM. Similar results were also obtained by loading the recording patch pipettes with a solution containing only 0.1 mM ATP. Our patch-action potential clamp experiments, on the other hand, revealed that DNP induced the opening of time- and voltage-independent membrane channels, with a unitary conductance of 23 pS. The channels allowed for the passage of outward current in the voltage range of the action potential, and the increase in membrane patch conductance correlated with the observed shortening of action potential duration during DNP superfusion. Our experiments provide the first simultaneous recordings of action potentials and DNP-induced channel currents in guinea pig ventricular myocytes. Overall, the data provide new evidence for the understanding of the cellular and subcellular mechanisms involved in the development of slow conduction velocity and propagation block after metabolic blockade.
authors.affiliation: Department of Pharmacology, SUNY Health Science Center, Syracuse 13210.
authors.forename: G E
authors.initials: GE
authors.lastname: Morley
authors.forename: J M
authors.initials: JM
authors.lastname: Anumonwo
authors.forename: M
authors.initials: M
authors.lastname: Delmar
mesh_terms: D019297:2,4-Dinitrophenol; D000200:Action Potentials; D000255:Adenosine Triphosphate / Q000494:pharmacology; D000332:Aerobiosis; D000818:Animals; D002450:Cell Communication / Q000187:drug effects; D004140:Dinitrophenols / Q000494:pharmacology*; D004533:Egtazic Acid / Q000494:pharmacology; D006168:Guinea Pigs; D006531:HEPES / Q000494:pharmacology; D006352:Heart Ventricles / Q000166:cytology / Q000378:metabolism; D066298:In Vitro Techniques; D007365:Intercellular Junctions / Q000187:drug effects; D015221:Potassium Channels / Q000187:drug effects; D014475:Uncoupling Agents / Q000494:pharmacology*; D016276:Ventricular Function
chemical_list: D004140:Dinitrophenols; D015221:Potassium Channels; D014475:Uncoupling Agents; D004533:Egtazic Acid; D000255:Adenosine Triphosphate; D019297:2,4-Dinitrophenol; D006531:HEPES