Effect of n-3 and n-6 dietary fats on the lipoxygenase products from stimulated rat neutrophils.
Fish oil was fed to rats in combination with an equal amount of olive, sunflower or linseed (flax) oil in semisynthetic diets for 3 weeks. Following stimulation of isolated neutrophils with calcium ionophore the levels of leukotrienes (LT) were determined by HPLC. Graphical presentation of the resultant data show a direct linear relationship between LTB production and substrate concentration with no preferential conversion of n-3 or n-6 substrates. In addition the results highlighted the greater conversion of eicosapentaenoic acid (EPA) and arachidonic acid (AA) to 5-hydroxy metabolites in stimulated neutrophils. There is no suggestion in our results of inhibition of any of the enzymatic conversion steps between EPA or AA and LTB production by any of the dietary fatty acids except by altering the EPA/AA ratio in neutrophil membranes.
Gibson
R A
RA
Department of Paediatrics, Flinders Medical Centre, Bedford Park South Australia.
Neumann
M A
MA
James
M J
MJ
Hawkes
J S
JS
Hall
C
C
Cleland
L G
LG
D000818:Animals; D016718:Arachidonic Acid / Q000097:blood; D004042:Dietary Fats, Unsaturated / Q000494:pharmacology*; D015118:Eicosapentaenoic Acid / Q000097:blood; D005227:Fatty Acids / Q000097:blood; D066298:In Vitro Techniques; D015289:Leukotrienes / Q000097:blood; D008084:Lipoxygenase / Q000378:metabolism*; D008297:Male; D008563:Membrane Lipids / Q000097:blood; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
D004042:Dietary Fats, Unsaturated; D005227:Fatty Acids; D015289:Leukotrienes; D008563:Membrane Lipids; D016718:Arachidonic Acid; D015118:Eicosapentaenoic Acid; D008084:Lipoxygenase