A new technique for detecting oxytocinase activity in electrophoresis gels.
A sensitive staining method for the detection of oxytocinase (EC 3.4.11.3) activity in electrophoresis gels has been described. The method is based on the enzymatic release of p-nitroaniline (PNA) from two specific synthetic oxytocinase substrates, S-benzyl-L-cysteine-p-nitroanilide (BCN) and L-leucine-p-nitroanilide (LN), respectively. The PNA is then diazotized with sodium nitrite and subsequently coupled to a chromogen, N-(1-naphthyl)-ethylenediamine dihydrochloride (NED) to produce a deep pink/magenta colored azo-dye at the site of oxytocinase activity.
Roy
A C
AC
Department of Obstetrics and Gynaecology, National University of Singapore.
Saha
N
N
Tan
S M
SM
Kamarul
F Z
FZ
Ratnam
S S
SS
D000814:Aniline Compounds / Q000378:metabolism; D010122:Cystinyl Aminopeptidase / Q000378:metabolism*; D004586:Electrophoresis; D005029:Ethylenediamines; D005782:Gels; D006801:Humans; D007202:Indicators and Reagents; D008297:Male; D012661:Semen / Q000201:enzymology*; D012680:Sensitivity and Specificity; D013053:Spectrophotometry
D000814:Aniline Compounds; D005029:Ethylenediamines; D005782:Gels; D007202:Indicators and Reagents; C008588:N-(1-naphthyl)ethylenediamine; C019498:4-nitroaniline; D010122:Cystinyl Aminopeptidase