Determination of baicalin and baicalein in rat plasma by high-performance liquid chromatography with electrochemical detection.
A rapid and sensitive method, using electrochemical detection, has been developed for the determination of baicalin and baicalein, the flavonoid of Scutellariae radix, in rat plasma. Following separation by high-performance liquid chromatography, baicalin and baicalein were oxidized at a glassy carbon electrode to permit selective electrochemical detection. Absolute detection limits were found to be 5 ng/ml from 50 microliters of plasma for baicalin and 2 ng/ml from 100 microliters of plasma for baicalein. The resulting assays were suitable for pharmacokinetic studies of baicalin and baicalein in rats.
Wakui
Y
Y
Research Institute for Pharmacology, Tsumura & Co., Ibaraki, Japan.
Yanagisawa
E
E
Ishibashi
E
E
Matsuzaki
Y
Y
Takeda
S
S
Sasaki
H
H
Aburada
M
M
Oyama
T
T
D000818:Animals; D000890:Anti-Infective Agents / Q000097:blood* / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004563:Electrochemistry; D044950:Flavanones; D005419:Flavonoids / Q000097:blood* / Q000493:pharmacokinetics; D007408:Intestinal Absorption / Q000502:physiology; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
D000890:Anti-Infective Agents; D044950:Flavanones; D005419:Flavonoids; C038044:baicalin; C006680:baicalein