title: 13C-NMR spectral analysis of the structures of mouse immunoglobulin G1 carrying allotypes a and j.
abstract: A novel 13C nuclear magnetic resonance (NMR) method is described for the detection of subtle structural differences between mouse immunoglobulins carrying different allotypes. Fc fragments of mouse IgG1 antibodies carrying allotypes a and j have been selectively labeled with [1-13C]methionine. 13C-NMR spectra have shown that the microenvironment around Met-398 is significantly different for the two kinds of allotypes. Peptide mapping and amino acid sequence analyses have revealed that Val-406 of IgG1 carrying allotype a is substituted for Ile in the case of allotype j. X-ray crystallographic data indicate that Met-398 is in close spatial proximity to Val (Ile)-406. We therefore conclude that the 13C-NMR method can provide us with a novel spectroscopic probe for the structural characterization of allotypic markers.
authors.affiliation: Faculty of Pharmaceutical Sciences, University of Tokyo, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Kim
authors.forename: K
authors.initials: K
authors.lastname: Kato
authors.forename: A
authors.initials: A
authors.lastname: Higuchi
authors.forename: N
authors.initials: N
authors.lastname: Nomura
authors.forename: H
authors.initials: H
authors.lastname: Noguchi
authors.forename: Y
authors.initials: Y
authors.lastname: Arata
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D007126:Immunoglobulin Allotypes / Q000737:chemistry*; D007074:Immunoglobulin G / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data
chemical_list: D007126:Immunoglobulin Allotypes; D007074:Immunoglobulin G