title: Expression of protein kinase C subspecies in rat retina.
abstract: An extract of rat retina was subjected to Mono Q followed by chromatography on hydroxyapatite, and the protein kinase C (PKC) subspecies were identified by immunoblot and biochemical analysis. It was found that, although the relative activities assayed with myelin basic protein as a common phosphate acceptor vary greatly with one another, the alpha-, beta I-, beta II-, gamma-, delta-, epsilon-, zeta-, and another structurally unknown PKC subspecies are expressed in this tissue. Thus, the retina is a unique tissue which expresses most of the PKC subspecies so far identified in mammals.
authors.affiliation: Department of Biochemistry, Kobe University School of Medicine, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Fujisawa
authors.forename: K
authors.initials: K
authors.lastname: Ogita
authors.forename: N
authors.initials: N
authors.lastname: Saito
authors.forename: Y
authors.initials: Y
authors.lastname: Nishizuka
mesh_terms: D000818:Animals; D000918:Antibody Specificity; D015151:Immunoblotting; D007700:Kinetics; D008297:Male; D011493:Protein Kinase C / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012160:Retina / Q000201:enzymology*
chemical_list: D011493:Protein Kinase C