Comparison of cysticercus extract, cyst fluid and Taenia saginata extract for use in ELISA for serodiagnosis of neurocysticercosis.
Cysticercus cellulosae extract (CS), cyst fluid (CF), and an extract of Taenia saginata adult worm (TS) were evaluated for use in an enzyme-linked immunosorbent assay (ELISA) for serodiagnosis of human neurocysticercosis in Thai patients. ELISA sensitivity was found to be 78.13%, 81.25% and 62.50%, respectively. False positivity was 6.66% with CS and 0% with other antigens. CF gave positivity with a pooled visceral gnathostomiasis serum and 3 of 10 (30%) of angiostrongyliasis sera. CS produced weakly positive ELISA with pooled opisthorchiasis and visceral gnathostomiasis sera. TS gave weak positive ELISA with a pooled opisthorchiasis serum. It was concluded that CF was the best antigen for use in ELISA for serodiagnosis of human neurocysticercosis.
Morakote
N
N
Department of Parasitology, Faculty of Medicine, Chiang Mai University, Thailand.
Nawacharoen
W
W
Sukonthasun
K
K
Thammasonthi
W
W
Khamboonruang
C
C
D000293:Adolescent; D000328:Adult; D000818:Animals; D000947:Antigens, Helminth / Q000097:blood; D001927:Brain Diseases / Q000097:blood / Q000175:diagnosis* / Q000453:epidemiology; D003551:Cysticercosis / Q000097:blood / Q000175:diagnosis* / Q000453:epidemiology; D003552:Cysticercus / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods* / Q000592:standards; D005069:Evaluation Studies as Topic; D005260:Female; D006785:Hospitals, University; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D008297:Male; D008875:Middle Aged; D015995:Prevalence; D012680:Sensitivity and Specificity; D013621:Taenia / Q000737:chemistry*; D013785:Thailand / Q000453:epidemiology; D014020:Tissue Extracts
D000947:Antigens, Helminth; D007074:Immunoglobulin G; D014020:Tissue Extracts