title: Photosensitized oxidation of cholesterol in biological systems: reaction pathways, cytotoxic effects and defense mechanisms.
abstract: Cholesterol resembles other unsaturated lipids in being susceptible to peroxidative degradation when exposed to a sensitizing agent, exciting light of suitable wavelength and molecular oxygen. Selected hydroperoxides of cholesterol can be used as relatively convenient and reliable indicators of primary photochemical mechanisms, allowing a distinction to be made between free radical-mediated and singlet oxygen-mediated reactions. When generated in cell membranes, hydroperoxides of cholesterol and other lipids can have deleterious effects on membrane structure and function. Such damage may be exacerbated if these photoproducts undergo one-electron reduction to oxyl radicals which in turn initiate chain peroxidation reactions. Cells can resist these effects by using a membrane-based glutathione peroxidase to catalyze the two-electron reduction and detoxification of lipid hydroperoxides. Recent advances in our understanding of cholesterol photo-oxidation from the standpoints of (a) mechanistic information, (b) cytotoxicity and (c) cytoprotection are discussed in this article.
authors.affiliation: Department of Biochemistry, Medical College of Wisconsin, Milwaukee 53226.
authors.forename: A W
authors.initials: AW
authors.lastname: Girotti
mesh_terms: D000818:Animals; D002470:Cell Survival; D002784:Cholesterol / Q000378:metabolism* / Q000528:radiation effects; D005609:Free Radicals; D006801:Humans; D015227:Lipid Peroxidation; D008081:Liposomes; D010084:Oxidation-Reduction; D010545:Peroxides / Q000378:metabolism; D010777:Photochemistry
chemical_list: D005609:Free Radicals; D008081:Liposomes; D010545:Peroxides; D002784:Cholesterol