Factors affecting the in vitro evolution of a myeloma cell line.
A continuously growing plasma cell line has been established from the bone marrow of a multiple myeloma patient. Initial growth of the cells was dependent on the presence of bone marrow stromal cells. Following initial outgrowth the cells were maintained by transfer onto non-autocthonous bone marrow stromal cultures. Following approximately one year of continuous growth, a subline was derived which could be grown independently of feeder cells. These stromal-cell-independent myeloma cells nevertheless retained dependence for a growth factor present in stromal-cell-conditioned media. The relevant factor in the conditioned media was determined to be interleukin-6 (IL-6). The cells also ultimately became independent of the conditioned media. These latter cells were shown to contain mRNA for IL-6 and eventually began to secrete IL-6. This cell line has thus progressed from complete dependence on stromal cells to IL-6-dependent growth in the absence of stromal cells to complete self sufficient growth. This in vitro progression may reflect an in vivo pattern of myeloma development.
Scibienski
R J
RJ
Department of Medical Microbiology and Immunology, School of Medicine, University of California, Davis 95616.
Paglieroni
T
T
Caggiano
V
V
Lemongello
D
D
Gumerlock
P H
PH
Mackenzie
M R
MR
D001853:Bone Marrow / Q000378:metabolism / Q000473:pathology; D002455:Cell Division; D006801:Humans; D015850:Interleukin-6 / Q000096:biosynthesis / Q000235:genetics; D009101:Multiple Myeloma / Q000378:metabolism / Q000473:pathology*; D010950:Plasma Cells / Q000378:metabolism / Q000473:pathology; D012333:RNA, Messenger / Q000378:metabolism; D011971:Receptors, Immunologic / Q000378:metabolism; D019947:Receptors, Interleukin-6; D014407:Tumor Cells, Cultured / Q000378:metabolism / Q000473:pathology
D015850:Interleukin-6; D012333:RNA, Messenger; D011971:Receptors, Immunologic; D019947:Receptors, Interleukin-6