title: Polymerase chain reaction with double primer pairs for detection of human parvovirus B19 induced aplastic crises in family outbreaks.
abstract: Parvovirus B19 DNA can be detected by polymerase chain reaction with double primer pairs (nested PCR). Recent infection was documented by a retrospective serological study using Parvoscan-B19 enzyme linked immunosorbent assay (EIA) for detection of B19 human parvovirus IgM and IgG antibodies in serum or plasma specimens. In 3 families B19 outbreaks caused aplastic crises necessitating blood transfusion in 5 children and 1 adult with hereditary sphaerocytosis. Four members from 2 of the families had clinically overt haemolytic anaemia prior to the event. Two members in another family presented with an aplastic crisis disclosing the underlying chronic haemolytic disease. All 7 patients were identified as PCR positive in serum samples taken 3-14 days after the onset of symptoms. Comparison with dot blot hybridization revealed detectable DNA in only 2/3 PCR positive patients. Thus, nested PCR is more sensitive than the dot blot hybridization method and is therefore a suitable complement to the antibody assay for identifying recent B19 infection.
authors.affiliation: Department of Virology, National Bacteriological Laboratory, Stockholm, Sweden.
authors.forename: E
authors.initials: E
authors.lastname: Fridell
authors.forename: A N
authors.initials: AN
authors.lastname: Békássy
authors.forename: B
authors.initials: B
authors.lastname: Larsson
authors.forename: B M
authors.initials: BM
authors.lastname: Eriksson
mesh_terms: D000293:Adolescent; D000328:Adult; D000741:Anemia, Aplastic / Q000382:microbiology; D000914:Antibodies, Viral / Q000097:blood*; D001483:Base Sequence; D002648:Child; D002675:Child, Preschool; D004277:DNA, Single-Stranded; D004279:DNA, Viral / Q000097:blood*; D004196:Disease Outbreaks; D016731:Erythema Infectiosum / Q000150:complications / Q000175:diagnosis* / Q000276:immunology; D005192:Family Health; D005260:Female; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D016732:Parvovirus B19, Human / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D013103:Spherocytosis, Hereditary / Q000150:complications
chemical_list: D000914:Antibodies, Viral; D004277:DNA, Single-Stranded; D004279:DNA, Viral