[Automated assay method for protein C anticoagulant activity].
We developed an automated assay method for protein C anticoagulant activity to prolong the APTT measured with automated coagulation apparatus. The automated assay method required following three reagents. One was 0.025 mol/l calcium chloride solution. The other two were a protein C deficient plasma and a phospholipid plus protein C activator both of which constituted a recomposed protein C assay kit (BMY). 0-50% activities of protein C and clotting times were regressed on normal linear curve, while 25-150% activities and clotting times were regressed on logarithmic curve. The coefficients of variation were 2.9-3.4% (intra-assay) and 3.6-3.9% (inter-assay). A correlation of protein C activities between measured by automated assay method and by ordinary manual method was good (r = 0.954). Our data suggest that this automated assay is a useful method for measuring the protein C anticoagulant activities utilizing a coagulation apparatus.
Iijima
K
K
Department of Clinical Laboratory, Tottori University Hospital, Yonago.
Kameo
Y
Y
Hara
I
I
Murakami
F
F
Nakamura
K
K
D001780:Blood Coagulation Tests / Q000379:methods; D006801:Humans; D011486:Protein C / Q000032:analysis*
D011486:Protein C