Effective derosetting of mononuclear cells isolated by immunomagnetic beads.
A rapid and effective method for the derosetting of peripheral mononuclear cells (PMNC) isolated by CD8 monoclonal antibody (MoAb) coated immunomagnetic beads (IMB) is described. PMNC are positively selected and enriched by CD8-IMB and then derosetted from the IMB by a polyclonal antibody (PoAb) preparation against mouse Fab. Our experiments confirm the feasibility of this method with a high derosetting efficiency.
Schwella
N
N
Abteilung Innere Medizin, Universitätsklinikum Rudolf Virchow, Freie Universität Berlin, FRG.
Zingsem
J
J
Eckstein
R
R
D000911:Antibodies, Monoclonal / Q000276:immunology*; D016827:CD8 Antigens / Q000276:immunology; D006801:Humans; D016130:Immunophenotyping / Q000379:methods*; D008212:Lymphocyte Depletion; D008280:Magnetics; D009504:Neutrophils / Q000276:immunology*; D012397:Rosette Formation
D000911:Antibodies, Monoclonal; D016827:CD8 Antigens