Distinctive uptake of neutral red by mitotic cancer cells.
Neutral red stains both normal and cancer mitotic cells, but uptake by living mitotic cancer cells is distinctly higher than in normal cells. This new approach to cancer cell identification is demonstrated in 4 established tumorigenic cancer cell lines: human skin epidermoid carcinoma A431, mouse Cloudman malignant melanoma, human oral epidermoid carcinoma and rat hepatoma. Human Chang liver cells served as normal controls. With epidermal growth factor (EGF) prepulse, neutral red uptake is dramatically enhanced. The possibility of a causal relationship with M-phase specific phosphorylation is discussed.
Sit
K H
KH
Department of Anatomy, Faculty of Medicine, National University of Singapore, Kent Ridge.
Bay
B H
BH
Wong
K P
KP
D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000378:metabolism / Q000473:pathology; D002467:Cell Nucleus / Q000378:metabolism / Q000502:physiology; D004815:Epidermal Growth Factor / Q000032:analysis; D006801:Humans; D007399:Interphase / Q000502:physiology; D008099:Liver / Q000166:cytology / Q000378:metabolism; D008114:Liver Neoplasms, Experimental / Q000378:metabolism / Q000473:pathology; D008546:Melanoma, Experimental / Q000378:metabolism / Q000473:pathology; D008938:Mitosis / Q000502:physiology*; D009062:Mouth Neoplasms / Q000378:metabolism / Q000473:pathology; D009374:Neoplasms, Experimental / Q000378:metabolism* / Q000473:pathology; D009499:Neutral Red / Q000493:pharmacokinetics*; D051381:Rats; D012878:Skin Neoplasms / Q000378:metabolism / Q000473:pathology; D014407:Tumor Cells, Cultured
D009499:Neutral Red; D004815:Epidermal Growth Factor