Identification of equine chromosomes in horse x mouse somatic cell hybrids.
Giemsa-11 (G-11) staining and in situ hybridization were used to identify the equine chromosome complement of horse x mouse somatic cell hybrids. The presence of horse chromosomes in somatic cell hybrids was determined by differential G-11 staining. The slides were then destained and hybridized with biotinylated total horse (Equus caballus) genomic DNA without suppression. Fluorescence detection permitted rapid confirmation of horse chromosomal DNA in the hybrid cells.
Lear
T L
TL
Gluck Equine Research Center, Lexington, KY.
Trembicki
K A
KA
Ennis
R B
RB
D000818:Animals; D002875:Chromosomes; D004247:DNA / Q000032:analysis*; D015342:DNA Probes / Q000235:genetics; D005453:Fluorescence; D006736:Horses / Q000235:genetics*; D006822:Hybrid Cells; D051379:Mice; D009693:Nucleic Acid Hybridization
D015342:DNA Probes; D004247:DNA