title: Delineation of putative mechanisms involved in antibody-mediated clearance of rabies virus from the central nervous system.
abstract: The in vitro biological activities of several rabies virus-neutralizing monoclonal antibodies (mAbs) were compared with their ability to prevent a lethal rabies virus encephalomyelitis. The protective activity of a particular mAb in vivo did not correlate with its virus-neutralizing activity in vitro; rather it was related to the mAb's ability to inhibit virus spread from cell to cell and to restrict rabies virus RNA transcription. Since treatment of rabies virus-infected cells with virus-neutralizing mAbs results in an endocytosis of the antibody, we hypothesize that an antibody may exert its inhibitory activity even after uptake by the cell. Post-exposure treatment of rats with a mAb that inhibited both virus spread and virus RNA transcription in vitro resulted in viral clearance from the central nervous system and protected the animals against a lethal rabies virus infection.
authors.affiliation: Wistar Institute of Anatomy and Biology, Philadelphia, PA 19109.
authors.forename: B
authors.initials: B
authors.lastname: Dietzschold
authors.forename: M
authors.initials: M
authors.lastname: Kao
authors.forename: Y M
authors.initials: YM
authors.lastname: Zheng
authors.forename: Z Y
authors.initials: ZY
authors.lastname: Chen
authors.forename: G
authors.initials: G
authors.lastname: Maul
authors.forename: Z F
authors.initials: ZF
authors.lastname: Fu
authors.forename: C E
authors.initials: CE
authors.lastname: Rupprecht
authors.forename: H
authors.initials: H
authors.lastname: Koprowski
mesh_terms: D000199:Actins / Q000235:genetics; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000914:Antibodies, Viral / Q000276:immunology*; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D001921:Brain / Q000382:microbiology*; D002213:Capsid / Q000235:genetics; D002460:Cell Line; D005260:Female; D051379:Mice; D008813:Mice, Inbred ICR; D008969:Molecular Sequence Data; D009447:Neuroblastoma; D009500:Neutralization Tests; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification; D011818:Rabies / Q000276:immunology*; D011820:Rabies virus / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D051381:Rats; D011917:Rats, Inbred Lew; D014158:Transcription, Genetic; D014758:Viral Core Proteins / Q000235:genetics
chemical_list: D000199:Actins; D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D012367:RNA, Viral; D014758:Viral Core Proteins