title: Absorption, distribution, metabolism, and excretion of bacteria-derived hyaluronic acid in rats and rabbits.
abstract: The feasibility of using bacteria-derived hyaluronate solution as a viscous aid for anterior chamber surgery was examined by studying the pharmacokinetic behavior and metabolic fate of 14C-labelled material, following administration to rats and rabbits. Intravenously-administered HA disappeared rapidly from the blood of rabbits and rats with a mean t1/2 of 5.3 and 3.7 min, respectively. The labelled material has concomittantly accumulated in the liver, where it was digested to oligomeric sugar subunits; these were further utilized metabolically either for energy generation or for incorporation into new high molecular weight species. Metabolic cage studies has indicated that most of the 14C-HA label administered intravenously to rats was excreted as CO2 via the respiration within 24h, while a smaller portion was excreted in the urine. The disposition of viscous 14C-HA administered into the anterior eye chamber of rabbits was slow and followed first-order kinetics with a t1/2 of 10.5h. No degradation occurred in the aqueous humour. Low blood levels of 14C-labeled material were found during 72h after intra-ocular administration. The results indicate that the absorption, distribution, metabolism and excretion of bacteria-derived HA is similar to those of the currently used ophthalmic surgery HA aids extracted from rooster combs.
authors.affiliation: BioTechnology General Ltd., Kiryat Weizmann, Rehovot, Israel.
authors.forename: A
authors.initials: A
authors.lastname: Nimrod
authors.forename: E
authors.initials: E
authors.lastname: Ezra
authors.forename: N
authors.initials: N
authors.lastname: Ezov
authors.forename: G
authors.initials: G
authors.lastname: Nachum
authors.forename: B
authors.initials: B
authors.lastname: Parisada
mesh_terms: D000042:Absorption; D000818:Animals; D000867:Anterior Chamber / Q000187:drug effects; D002250:Carbon Radioisotopes; D002850:Chromatography, Gel; D006820:Hyaluronic Acid / Q000096:biosynthesis / Q000378:metabolism / Q000493:pharmacokinetics*; D007275:Injections, Intravenous; D008099:Liver / Q000378:metabolism; D008297:Male; D011817:Rabbits; D051381:Rats; D011919:Rats, Inbred Strains; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000378:metabolism / Q000493:pharmacokinetics; D013291:Streptococcus / Q000378:metabolism; D014018:Tissue Distribution
chemical_list: D002250:Carbon Radioisotopes; D011994:Recombinant Proteins; D006820:Hyaluronic Acid