Phospholipid import into mitochondria: possible regulation mediated through lipid polymorphism.
We previously demonstrated that the translocation of microsomal phosphatidylserine to the inner mitochondrial membrane occurs via contact sites before decarboxylation. According to the specific lipid composition of contact sites, we investigated lipid polymorphism as a possible regulation mechanism of phospholipid import into mitochondria. Phosphatidylserine import into mitochondria is increased in the presence of calcium, under conditions where non bilayer lipid-structures can be induced in cardiolipin-containing membranes. The results are discussed in terms of structural as well as functional domains heterogeneity within contact sites.
Ardail
D
D
Department of Biochemistry, Lyon-Sud Medical School, INSERM-CNRS U. 189, Oullins, France.
Lermé
F
F
Louisot
P
P
D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002250:Carbon Radioisotopes; D002499:Centrifugation, Density Gradient; D004317:Doxorubicin / Q000494:pharmacology; D007425:Intracellular Membranes / Q000378:metabolism*; D007700:Kinetics; D008563:Membrane Lipids / Q000302:isolation & purification / Q000378:metabolism*; D051379:Mice; D008862:Microsomes, Liver / Q000378:metabolism*; D008930:Mitochondria, Liver / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D010718:Phosphatidylserines / Q000378:metabolism*; D012694:Serine / Q000378:metabolism; D013367:Submitochondrial Particles / Q000378:metabolism*
D002250:Carbon Radioisotopes; D008563:Membrane Lipids; D010718:Phosphatidylserines; D012694:Serine; D004317:Doxorubicin; D002118:Calcium