Detection and time-course analysis of phospholipid hydroperoxide in soybean seedlings after treatment with fungal elicitor, by chemiluminescence-HPLC assay.
Direct evidence for the existence of phosphatidylcholine hydroperoxide (PCOOH) and the determination of the changes in PCOOH after treatment of soybean seedlings with fungal elicitor, were examined using chemiluminescence-HPLC of hydroperoxide specific assay. PCOOH was transiently accumulated within 6 h after elicitor treatment but such an event was not observed in control plants, thus suggesting that accumulation of PCOOH was a response to fungal elicitor in the host plant. The result suggested that singlet oxygen had participated in the lipid hydroperoxidation, since non-conjugated fatty acid hydroperoxide isomers due to the singlet oxygen oxidation were detected in the phospholipid fraction. The reaction of lipid hydroperoxidation, preceding the induction of a defense compound, may be involved in the triggering of a plant defense mechanism against fungal invasion.
Kondo
Y
Y
Mizutani Plant Ecochemicals Project, Research Development Corporation of Japan, Eniwa.
Miyazawa
T
T
Mizutani
J
J
D001578:Benzopyrans / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002855:Chromatography, Thin Layer; D005656:Fungal Proteins / Q000302:isolation & purification / Q000494:pharmacology*; D007700:Kinetics; D008163:Luminescent Measurements; D010713:Phosphatidylcholines / Q000302:isolation & purification / Q000378:metabolism*; D010838:Phytophthora; D036343:Pterocarpans; D013025:Glycine max / Q000187:drug effects / Q000378:metabolism*; D013997:Time Factors
D001578:Benzopyrans; D005656:Fungal Proteins; D010713:Phosphatidylcholines; D036343:Pterocarpans; C057590:phosphatidylcholine hydroperoxide; C017343:glyceollin