Determination of procaine in equine plasma and urine by high-performance liquid chromatography.
The variability in plasma and urine equine procaine measurement between three independent laboratories using current methods led to the development of a sensitive, reliable, and reproducible high-performance liquid chromatographic method. Standardbred mares were administered either a penicillin G procaine preparation intramuscularly or procaine hydrochloride subcutaneously, and blood and urine were collected at defined time intervals. By HPLC the detection limits for procaine in plasma and urine were 1 and 10 ng/mL, respectively. In contrast procaine in plasma could not be detected by GC-NPD, while the urinary detection limit was 50 ng/mL. The concentration of fluoride in the collection tubes and repetitive freeze-thawing modified plasma procaine measurement. Urinary pH was a factor in estimation of urine procaine levels with greater recovery and reproducibility of results at pH 5 as compared to pH 7. This HPLC method provides a simple, sensitive, and reliable quantitation of procaine in equine plasma and urine.
Stevenson
A J
AJ
Race Track Division, Agriculture Canada, Ottawa and Jerseyville, Ontario.
Weber
M P
MP
Todi
F
F
Mendonca
M
M
Fenwick
J D
JD
Young
L
L
Kwong
E
E
Chen
F
F
Beaumier
P
P
Timmings
S
S
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000662:veterinary*; D004300:Doping in Sports; D006736:Horses / Q000378:metabolism*; D011343:Procaine / Q000032:analysis* / Q000097:blood / Q000652:urine
D011343:Procaine