title: Leakage of carbonic anhydrase III from normal and denervated rat skeletal muscle following contractile activity.
abstract: Skeletal muscle extracellular carbonic anhydrase III was investigated in anesthetized rats by a microdialysis technique. A small dialysis probe was inserted into the tibialis anterior (TA) muscle and perfused continuously. Perfusates were collected before and during muscle contraction, induced by electrical stimulation of the muscle or of the sciatic nerve. In the perfusate of resting normal and denervated muscle, the concentration of CA III was 10 to 12 ng/mL, as measured by a radioimmunosorbent technique. During contractile activity, the concentrations of CA III increased markedly in the normal and denervated muscle. A TA muscle suspended in physiological saline behaved similarly, even though the leakage before and during contraction was higher than in vivo. The results show that skeletal muscle leaks CA III both in vivo and in vitro, a leakage which was markedly increased by contractile activity. The microdialysis technique should also be useful in humans to study the efflux of various proteins from different kinds of diseased or fatigued muscles.
authors.affiliation: Department of Neurology, Uppsala University, Sweden.
authors.forename: H
authors.initials: H
authors.lastname: Askmark
authors.forename: P J
authors.initials: PJ
authors.lastname: Wistrand
mesh_terms: D000818:Animals; D002256:Carbonic Anhydrases / Q000378:metabolism*; D004558:Electric Stimulation; D008297:Male; D009119:Muscle Contraction / Q000502:physiology*; D009121:Muscle Denervation; D009132:Muscles / Q000201:enzymology*; D010477:Perfusion; D011864:Radioimmunosorbent Test; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D002256:Carbonic Anhydrases