title: Leishmania major and Leishmania donovani: effect of LPG-containing and LPG-deficient strains on monocyte chemotaxis and chemiluminescence.
abstract: Lipophosphoglycan (LPG) is a major glycolipid present on the membrane of Leishmania promastigotes and amastigotes. We have previously shown that preincubation of peripheral blood monocytes with purified LPG inhibits IL-1 production, chemotactic locomotion, and luminol-dependent chemiluminescence (LDCL). In the present study we tested the effect of LPG present on live parasites on monocyte activity. For this purpose, we used two mutant strains deficient in LPG and two LPG-containing strains. One pair was Leishmania major and the other Leishmania donovani. Monocytes in suspension were infected with the different parasite strains and tested for chemotactic locomotion and LDCL at different times between 1 and 72 hr after infection. In parallel, the percentage of infected monocytes was measured in stained cytospin preparations. The results obtained showed that at 1 hr of incubation only the LPG-containing strains inhibited chemotaxis, while the mutant strains showed a normal response. From 4 hr of incubation onwards, the mutant strains also inhibited monocyte chemotactic locomotion. LDCL was only slightly inhibited by the LPG-containing strains after 1 hr, because of a high level of spontaneous stimulation, probably due to phagocytosis. At 24 and 72 hr all strains inhibited LDCL. These results suggest that LPG is responsible for early inhibition of macrophage activity, but that other factors are responsible for inhibition at later stages of in vitro infection. The model described here might represent a useful tool to further analyze the mechanisms involved in immune evasion of Leishmania parasites.
authors.affiliation: Department of Dermatology, Hadassah University Hospital, Jerusalem, Israel.
authors.forename: S
authors.initials: S
authors.lastname: Frankenburg
authors.forename: A
authors.initials: A
authors.lastname: Gross
authors.forename: V
authors.initials: V
authors.lastname: Leibovici
mesh_terms: D000818:Animals; D002634:Chemotaxis, Leukocyte; D006028:Glycosphingolipids / Q000502:physiology*; D006801:Humans; D066298:In Vitro Techniques; D007893:Leishmania donovani / Q000502:physiology*; D007895:Leishmania tropica / Q000502:physiology*; D008163:Luminescent Measurements; D009000:Monocytes / Q000469:parasitology* / Q000502:physiology
chemical_list: D006028:Glycosphingolipids; C008290:lipophosphonoglycan