title: Subcellular fractionation evidence for a putative peroxisome-mitochondrion attachment in the liver of normal and genetically obese (ob/ob and db/db) mice.
abstract: 1. Liver post-nuclear supernatants (PNS) from several mouse strains were fractionated by zonal centrifugation and fractions analysed by marker-enzyme estimations+electron microscopy. 2. Rate-dependent banding of PNS yielded peroxisome-enriched (PER) and mitochondrion-enriched (MER) regions. 3. Density-dependent banding of PER yielded peroxisomes (approximately 1.22 g/ml) well separated from mitochondria (approximately 1.8 g/ml). 4. Density-dependent banding of MER yielded peroxisomes that co-distributed with mitochondria and electron microscopy revealed close proximity of the two organelles. 5. Experiments demonstrated that co-distribution was not due to weak binding of proteins or to agglutination of organelles. 6. The results indicate in vivo attachment of some mitochondria and peroxisomes.
authors.affiliation: School of Applied Sciences, Wolverhampton Polytechnic, U.K.
authors.forename: V M
authors.initials: VM
authors.lastname: Mann
authors.forename: V U
authors.initials: VU
authors.lastname: Nwosu
authors.forename: A
authors.initials: A
authors.lastname: Silcox
authors.forename: C J
authors.initials: CJ
authors.lastname: Jones
authors.forename: K
authors.initials: K
authors.lastname: Burdett
authors.forename: M J
authors.initials: MJ
authors.lastname: Connock
mesh_terms: D000818:Animals; D015415:Biomarkers; D002458:Cell Fractionation; D002499:Centrifugation, Density Gradient; D005260:Female; D008099:Liver / Q000201:enzymology / Q000648:ultrastructure; D008297:Male; D051379:Mice; D008820:Mice, Obese; D008830:Microbodies / Q000201:enzymology / Q000648:ultrastructure*; D008854:Microscopy, Electron; D008930:Mitochondria, Liver / Q000201:enzymology / Q000648:ultrastructure*
chemical_list: D015415:Biomarkers