Specific antigen binding by activated cerebrospinal fluid B lymphocytes in acute neuroborreliosis.
A recently developed immunocytochemical antigen-binding sandwich test for the identification of specific activated B lymphocytes was applied to cerebrospinal fluid cells and peripheral blood mononuclear cells in patients with acute neuroborreliosis. Discrimination of antigen-binding phagocytes was achieved by double staining with monoclonal antibodies. Specific activated B lymphocytes were much more numerous in cerebrospinal fluid than in blood, showing great interindividual differences. When intrathecal immunoglobulin production was present, the number of specific activated B lymphocytes was also high. The specificity of all activated B lymphocytes ranged from 10% to 60% and was higher in the acute stage than after treatment.
Beuche
W
W
Abteilung für Neurologie, Universität, Göttingen, Federal Republic of Germany.
Siever
A
A
Felgenhauer
K
K
D000208:Acute Disease; D000368:Aged; D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D015748:Borrelia burgdorferi Group / Q000276:immunology; D005260:Female; D006801:Humans; D008193:Lyme Disease / Q000134:cerebrospinal fluid* / Q000276:immunology*; D008213:Lymphocyte Activation / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D051381:Rats; D011919:Rats, Inbred Strains; D012680:Sensitivity and Specificity