Automated lanes detection and comparison of bacterial electrophoretic protein fingerprints using fast Fourier transformation.
A method of computer-automated analysis of bacterial fingerprints produced by electrophoresis of proteins in a one-dimensional slab gel system is described. Proteins were visualized by silver staining. Western blotting, or autoradiography. Gels were recorded with a CCD camera, and after initial manual removal of the unwanted image margins, track margins were identified and extracted and a normalized trace was produced automatically using Fourier routines to smooth plots required for this process. Normalized traces were then compared by Fourier correlation after application of a high-pass step filter.
Millership
S
S
Department of Bacteriology, Royal Postgraduate Medical School, Hammersmith Hospital, London, UK.
Ragoonaden
K
K
D000333:Aeromonas / Q000737:chemistry / Q000302:isolation & purification; D001345:Autoradiography; D001426:Bacterial Proteins / Q000032:analysis*; D015153:Blotting, Western; D016360:Clostridioides difficile / Q000737:chemistry / Q000302:isolation & purification; D001330:Electronic Data Processing; D004591:Electrophoresis, Polyacrylamide Gel; D005583:Fourier Analysis; D007091:Image Processing, Computer-Assisted; D008970:Molecular Weight; D016622:Silver Staining; D013211:Staphylococcus aureus / Q000737:chemistry / Q000302:isolation & purification
D001426:Bacterial Proteins