title: Heat stability of parvovirus B19: kinetics of inactivation.
abstract: Heat inactivation of parvovirus B19 (B19) was studied in a culture of hematopoietic progenitor cells generated in vitro from peripheral human blood. After inoculating cell cultures with identical volumes of plasma (MII) containing B19 (B19-MII) heat-treated (60 degrees C) for various periods of time, a time-dependent inactivation of the input virus was determined by a decrease of viral DNA replication. No B19 DNA was detected after infection with B19-MII heat-treated for 20 min or more by Southern blot. Viral B19 protein production decreased time-dependently and was not detected after infection with samples treated for 12 min at 60 degrees C or more determined by the enzyme immunoassay. This study indicates that infectivity of B19 virus in plasma can be reduced in vitro by heat-treatment (60 degrees C). However, this does not mean that the heat treatment completely inactivated B19 virus.
authors.affiliation: Max von Pettenkofer-Institut für Hygiene und Medizinische Mikrobiologie, Ludwig-Maximilians-Universität, München, Germany.
authors.forename: T F
authors.initials: TF
authors.lastname: Schwarz
authors.forename: S
authors.initials: S
authors.lastname: Serke
authors.forename: A
authors.initials: A
authors.lastname: Von Brunn
authors.forename: B
authors.initials: B
authors.lastname: Hottenträger
authors.forename: D
authors.initials: D
authors.lastname: Huhn
authors.forename: F
authors.initials: F
authors.lastname: Deinhardt
authors.forename: M
authors.initials: M
authors.lastname: Roggendorf
mesh_terms: D002478:Cells, Cultured; D004261:DNA Replication / Q000502:physiology; D004279:DNA, Viral / Q000502:physiology; D006358:Hot Temperature; D006801:Humans; D007700:Kinetics; D016732:Parvovirus B19, Human / Q000502:physiology*; D014764:Viral Proteins / Q000096:biosynthesis; D014779:Virus Replication / Q000502:physiology
chemical_list: D004279:DNA, Viral; D014764:Viral Proteins