Identification of O-methyldopa in the ventricular fluid of patients with Parkinson's disease.
A newly developed liquid chromatographic system with a multiple electrode detector was used for quantification of neurochemical substances in ventricular fluid of patients with Parkinson's disease. During the analysis, an unknown peak was observed at almost the same high-performance liquid chromatography retention time as 5-hydroxytryptophan. Through the use of ion suppression techniques, voltammographic analysis and fast atom bombardment mass spectrometry, the compound was identified as O-methyldopa, the major metabolite of L-dopa.
Ikarashi
Y
Y
Department of Neuropsychopharmacology (Tsumura), Gunma University, School of Medicine, Japan.
Maruyama
Y
Y
D002552:Cerebral Ventricles / Q000737:chemistry / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid; D004563:Electrochemistry; D006801:Humans; D007202:Indicators and Reagents; D008750:Methyldopa / Q000134:cerebrospinal fluid*; D010300:Parkinson Disease / Q000134:cerebrospinal fluid*; D012701:Serotonin / Q000378:metabolism; D016339:Spectrometry, Mass, Fast Atom Bombardment
D007202:Indicators and Reagents; D012701:Serotonin; D008750:Methyldopa