title: Evolutionary divergence plots of homologous proteins.
abstract: A simple and efficient method is described for analyzing quantitatively multiple protein sequence alignments and finding the most conserved blocks as well as the maxima of divergence within the set of aligned sequences. It consists of calculating the mean distance and the root-mean-square distance in each column of the multiple alignment, averaging the values in a window of defined length and plotting the results as a function of the position of the window. Due attention is paid to the presence of gaps in the columns. Several examples are provided, using the sequences of several cytochromes c, serine proteases, lysozymes and globins. Two distance matrices are compared, namely the matrix derived by Gribskov and Burgess from the Dayhoff matrix, and the Risler Structural Superposition Matrix. In each case, the divergence plots effectively point to the specific residues which are known to be essential for the catalytic activity of the proteins. In addition, the regions of maximum divergence are clearly delineated. Interestingly, they are generally observed in positions immediately flanking the most conserved blocks. The method should therefore be useful for delineating the peptide segments which will be good candidates for site-directed mutagenesis and for visualizing the evolutionary constraints along homologous polypeptide chains.
authors.affiliation: Centre de Génétique Moléculaire du CNRS, Laboratoire Associé à l'Université P et M Curie, Gif-sur-Yvette, France.
authors.forename: S
authors.initials: S
authors.lastname: Brouillet
authors.forename: J L
authors.initials: JL
authors.lastname: Risler
authors.forename: A
authors.initials: A
authors.lastname: Hénaut
authors.forename: P P
authors.initials: PP
authors.lastname: Slonimski
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D005075:Biological Evolution; D008969:Molecular Sequence Data; D011506:Proteins / Q000737:chemistry* / Q000145:classification* / Q000235:genetics; D016415:Sequence Alignment / Q000379:methods; D012689:Sequence Homology, Nucleic Acid; D012984:Software
chemical_list: D011506:Proteins