Automated high-performance liquid chromatography of iopentol in human plasma and whole blood using on-line dialysis as sample preparation.
A fully automated high-performance liquid chromatographic method has been developed for the analysis of the radiographic contrast agent iopentol in human plasma and whole blood. This method is based on on-line sample preparation with dialysis followed by pre-column enrichment of the dialysate. The method was compared with a manual sample preparation method based on protein precipitation with tetrahydrofuran. The inter-assay and intra-assay variations and the limits of quantitation were the same for both methods. The on-line combination of dialysis and column-switching high-performance liquid chromatography was shown to be a reliable and time-saving technique for monitoring iopentol in human whole blood and plasma.
Andresen
A T
AT
Institute of Pharmacy, University of Oslo, Norway.
Jacobsen
P B
PB
Rasmussen
K E
KE
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003956:Dialysis / Q000379:methods; D006801:Humans; D010949:Plasma / Q000737:chemistry*; D014283:Triiodobenzoic Acids / Q000032:analysis* / Q000097:blood*
D014283:Triiodobenzoic Acids; C053571:iopentol