The growth arrest-specific gene, gas1, is involved in growth suppression.
This report describes the structure of the mRNA, the protein product, and the growth-regulating activity of one of the growth arrest-specific genes, gas1. From the predicted amino acid sequence, in vitro translation of gas1 mRNA, and immunofluorescence of cells in culture, it appears that the gas1 protein is an integral plasma membrane protein whose expression is linked to growth arrest. When gas1 is overexpressed from a constitutive promoter in quiescent cells, the serum-induced transition from the G0 to the S phase of the cell cycle is inhibited without affecting the normal early serum response. Ectopic expression of the gas1 gene by microinjection in normal and transformed NIH 3T3 cell lines with the notable exception of SV40-transformed 3T3 cells leads to inhibition of DNA synthesis. Thus, gas1 appears to be one component of a negative circuit that governs growth suppression. Its effect is, however, abolished in SV40-transformed cells.
Del Sal
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G
International Centre for Genetic Engineering and Biotechnology, Consortium for Interuniversity Biotechnology Laboratories, Trieste, Italy.
Ruaro
M E
ME
Philipson
L
L
Schneider
C
C
D016475:3T3 Cells / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018797:Cell Cycle Proteins; D058851:GPI-Linked Proteins; D005796:Genes; D015698:Genomic Library; D006128:Growth / Q000235:genetics; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000235:genetics* / Q000494:pharmacology; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics* / Q000494:pharmacology; D051379:Mice; D008938:Mitosis / Q000187:drug effects / Q000235:genetics*; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000737:chemistry; D029701:Saccharomyces cerevisiae Proteins
D018797:Cell Cycle Proteins; C066709:GAS1 protein, S cerevisiae; C096192:GAS1 protein, human; D058851:GPI-Linked Proteins; C501180:Gas1 protein, mouse; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D012333:RNA, Messenger; D029701:Saccharomyces cerevisiae Proteins