Development and use of a stability-indicating high-performance liquid chromatographic assay for Meldrum's acid.
A high-performance liquid chromatographic method was developed that separates Meldrum's acid from its primary decomposition products, malonic acid and acetone. The method uses a reversed-phase column under isocratic conditions, with detection by ultraviolet absorption at 210 nm. Quantitation of the parent molecule and the acid decomposition product was possible over concentration ranges of 0.1-10.0 and 0.1-2.0 mg/ml, respectively. Acetone could be determined only at much higher concentrations. Using the malonic acid concentration as a measure of decomposition, this method was used to determine the hydrolytic stability of Meldrum's acid and its skin penetration properties.
Kaczvinsky
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JR
Procter & Gamble Company, Hair & Skin Care Technology Division, Miami Valley Laboratories, Cincinnati, OH 45239-8707.
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D000096:Acetone / Q000032:analysis / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004146:Dioxanes / Q000032:analysis*; D006801:Humans; D008314:Malonates / Q000032:analysis / Q000493:pharmacokinetics; D012867:Skin / Q000737:chemistry; D012869:Skin Absorption / Q000502:physiology; D012879:Skin Physiological Phenomena; D014466:Ultraviolet Rays
D004146:Dioxanes; D008314:Malonates; D000096:Acetone; C076489:Meldrum's acid