Immortalization of murine B cells in vitro with oncogene-containing retroviral vectors.
A large panel of oncogene-containing retroviral vectors has been constructed and used to infect activated murine splenic B cells to determine whether particular oncogenes are capable of directly mediating B cell immortalization. Mature B cell lines have been consistently established with some of these retroviral vectors. These B cell lines arose at a low frequency, indicating that more genetic events were required in addition to infection with the retroviral vector for immortalization to occur. All such lines were LPS-dependent and non-tumorigenic. All lines secrete IgG and express surface IgG, but not IgD or IgM. In addition, they are CD11b+ and CD23-. These cells may be derived from the CD5 "lineage" or a related B cell subset and appear to be more susceptible to immortalization than conventional B cells.
Grabstein
K H
KH
Immunex Corporation, Seattle, WA 98101.
Hess
B
B
Weisser
K E
KE
Clark
L
L
Goodwin
R
R
Overell
R W
RW
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D005822:Genetic Vectors; D008213:Lymphocyte Activation; D051379:Mice; D008810:Mice, Inbred C57BL; D016374:Oncogene Proteins v-abl / Q000235:genetics; D009857:Oncogenes; D012190:Retroviridae / Q000235:genetics*
D016374:Oncogene Proteins v-abl