Counter-current chromatography of lipoproteins with a polymer phase system using the cross-axis synchronous coil planet centrifuge.
Lipoproteins were separated by counter-current chromatography using the type-XLL coil planet centrifuge. The separation was performed with a polymer phase system composed of 16% (w/w) polyethylene glycol 1000 and 12.5% (w/w) dibasic potassium phosphate by eluting the lower phase at a flow-rate of 0.5 ml/min. About 5 ml of the sample solution containing approximately 150 mg of a lipoprotein mixture were loaded. High- and low-density lipoproteins were resolved within 12 h. Each component was detected by gel electrophoresis with oil red staining.
Shibusawa
Y
Y
Laboratory of Biophysical Chemistry, National Heart, Lung, and Blood Institute, Bethesda, MD 20892.
Ito
Y
Y
Ikewaki
K
K
Rader
D J
DJ
Brewer
H B
HB
D002498:Centrifugation; D002845:Chromatography / Q000295:instrumentation / Q000379:methods*; D005914:Globins / Q000302:isolation & purification; D006801:Humans; D008074:Lipoproteins / Q000097:blood*; D008075:Lipoproteins, HDL / Q000097:blood; D008077:Lipoproteins, LDL / Q000097:blood; D011092:Polyethylene Glycols; D012709:Serum Albumin / Q000302:isolation & purification
D008074:Lipoproteins; D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL; D012709:Serum Albumin; D011092:Polyethylene Glycols; D005914:Globins