title: Crystallization and preliminary crystallographic studies of glucosamine-6-phosphate deaminase from Escherichia coli K12.
abstract: Hexameric glucosamine-6-phosphate deaminase from Escherichia coli has been crystallized isomorphously with both phosphate and ammonium sulphate as precipitants, over a wide pH range (6.0 to 9.0). The crystals belong to space group R32 and the cell parameters in the hexagonal setting are a = b = 125.9 A and c = 223.2 A. A complete native data set was collected to 2.1 A resolution. Self-rotation function studies suggest that the hexamers sit on the 3-fold axis and have point group symmetry 32, with a non-crystallographic dyad relating two monomers linked by an interchain disulfide bridge. A possible packing for the unit cell is proposed.
authors.affiliation: Instituto de Física e Química de São Carlos, Universidade de São Paulo, Brazil.
authors.forename: E
authors.initials: E
authors.lastname: Horjales
authors.forename: M M
authors.initials: MM
authors.lastname: Altamirano
authors.forename: M L
authors.initials: ML
authors.lastname: Calcagno
authors.forename: Z
authors.initials: Z
authors.lastname: Dauter
authors.forename: K
authors.initials: K
authors.lastname: Wilson
authors.forename: R C
authors.initials: RC
authors.lastname: Garratt
authors.forename: G
authors.initials: G
authors.lastname: Oliva
mesh_terms: D019747:Aldose-Ketose Isomerases; D000494:Allosteric Regulation / Q000502:physiology; D002238:Carbohydrate Epimerases / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification; D003460:Crystallization; D004926:Escherichia coli / Q000201:enzymology*; D014961:X-Ray Diffraction
chemical_list: C020582:glucosamine-6-phosphate isomerase; D002238:Carbohydrate Epimerases; D019747:Aldose-Ketose Isomerases