title: A mathematical approach to lipolysis based on the interrelationship of physicochemical and biochemical data.
abstract: A novel approach is described for the quantitation of the parameters involved in lipolytic activity. The activity of lipase purified from human pancreatic juice against an oil-water emulsion depends on the degree of emulsification, the type of emulsifier used, the type and the concentration of bile salt, the concentration of colipase and, finally, on the zeta-potential of the interface. The zeta-potential, in turn, depends on the partition of bile salt molecules between the micellar state in the bulk and their adsorption on the oil-water interface. None of these parameters correlates straightforward with lipase activity. However, if several parameters are taken into account, the enzyme activity determined in our system obeys a multiparametric equation where the variables are: the conjugation of bile salts, the number of hydroxy groups, their aggregation number and, finally, the zeta-potential of the oil droplets.
authors.affiliation: Laboratory of Biochemistry, School of Chemistry, Aristotelian University of Thessaloniki, Greece.
authors.forename: A
authors.initials: A
authors.lastname: Tavridou
authors.forename: A
authors.initials: A
authors.lastname: Avranas
authors.forename: P
authors.initials: P
authors.lastname: Arzoglou
mesh_terms: D001647:Bile Acids and Salts / Q000494:pharmacology*; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D004655:Emulsions; D006801:Humans; D007700:Kinetics; D008049:Lipase / Q000302:isolation & purification / Q000378:metabolism*; D008066:Lipolysis; D008433:Mathematics; D010189:Pancreatic Juice / Q000201:enzymology*; D014461:Ultracentrifugation
chemical_list: D001647:Bile Acids and Salts; D004655:Emulsions; D008049:Lipase