Binding of fibronectin and type II collagen to Staphylococcus aureus from bovine mastitis: reduction of binding after growth in milk whey.
The binding of fibronectin and type II collagen to Staphylococcus aureus strains isolated from bovine mastitis was found to be 20-80% lower for organisms grown in milk whey compared to those grown in tryptic soy broth (TSB). The reduced binding was accompanied by reduced surface hydrophobicity. The observed changes, after growth in milk whey, were not due to a mere adsorption of milk whey components. The binding of fibronectin and the degree of surface hydrophobicity of milk whey-grown bacteria became similar to that of TSB-grown bacteria after periodate treatment, whereas trypsin or papain treatments had no effect.
Mamo
W
W
Department of Veterinary Microbiology, Faculty of Veterinary Medicine, Swedish University of Agricultural Sciences, Uppsala.
Lindahl
M
M
Jonsson
P
P
D000818:Animals; D001422:Bacterial Adhesion / Q000502:physiology; D002417:Cattle; D003094:Collagen / Q000378:metabolism*; D003470:Culture Media; D005353:Fibronectins / Q000378:metabolism*; D008321:Mammary Glands, Animal / Q000382:microbiology; D008414:Mastitis, Bovine / Q000382:microbiology*; D008892:Milk / Q000382:microbiology*; D011485:Protein Binding; D013211:Staphylococcus aureus / Q000254:growth & development / Q000378:metabolism* / Q000472:pathogenicity
D003470:Culture Media; D005353:Fibronectins; D003094:Collagen