title: Maintenance and synthesis of proteins for an anucleate axon.
abstract: The anucleate (distal) segment of a crayfish medial giant axon (MGA) remains intact for months in vivo after severing the axon from its cell body, a phenomenon referred to as long-term survival (LTS). We collected axoplasm from chronic anucleate MGAs by perfusing 2-cm lengths of axons with an intracellular saline. This axoperfusate was analyzed by SDS-PAGE and silver stained. Axoperfusate proteins from intact MGAs and from chronic anucleate MGAs exhibiting LTS for up to 6 months were the same. Furthermore, immunoreactive levels of actin and beta-tubulin were similar in axoperfusates from intact and chronic anucleate MGAs. This maintenance of proteins in chronic anucleate MGAs must be due to a lack of protein degradation and/or to local protein synthesis by a source other than the cell body. To investigate local protein synthesis in vitro, we added [35S]-methionine to the extracellular saline surrounding intact and chronic anucleate MGAs. After 4- to 6-h incubations, radiolabelled proteins were detected in axoperfusates analyzed by SDS-PAGE and fluorography. The similarity between radiolabelled proteins in axoperfusates and MGA glial sheaths indicated a glial origin for the radiolabelled axoperfusate proteins. Various observations and control experiments suggested that glial-axonal protein transfer occurred by a physiological process. Glial-axonal protein transfer may contribute to the maintenance of proteins during LTS of chronic anucleate MGAs.
authors.affiliation: Department of Zoology, University of Texas, Austin 78712.
authors.forename: R A
authors.initials: RA
authors.lastname: Sheller
authors.forename: G D
authors.initials: GD
authors.lastname: Bittner
mesh_terms: D000818:Animals; D016477:Artifacts; D003400:Astacoidea / Q000378:metabolism*; D001369:Axons / Q000378:metabolism* / Q000648:ultrastructure; D002467:Cell Nucleus / Q000648:ultrastructure; D009419:Nerve Tissue Proteins / Q000096:biosynthesis*; D010477:Perfusion; D013462:Sulfur Radioisotopes
chemical_list: D009419:Nerve Tissue Proteins; D013462:Sulfur Radioisotopes