title: Patterns of immunocytochemically detected Z-DNA in the recrudescing testicular epithelium of the Turkish hamster (Mesocricetus brandti).
abstract: Z-DNA has been considered a labile but essential structural form of DNA in recombination and gene expression, two significant activities in mammalian seminiferous epithelium. The present study has utilized the recrudescing testes of Mesocricetus brandti to study in detail the potential Z-DNA sites in specific testicular cell types as detected by an immunoprobe. Testicular regression was physiologically induced by modifying environmental photoperiods and/or temperature. Partial atrophy of seminiferous epithelium occurred in all experimental groups but Sertoli cells persisted throughout regression. Recrudescence of testicular activity was marked in all experimental groups by characteristic sequences of reappearance of potential Z-DNA sites to a final positive or negative mature state of the cell type. It is suggested that Z-DNA is a functionally important from of DNA in many cell types of the active seminiferous epithelium of the Turkish hamster, and perhaps other mammals.
authors.affiliation: Center for Reproductive Sciences, Columbia University College of Physicians and Surgeons, New York, NY 10032.
authors.forename: G M
authors.initials: GM
authors.lastname: Jagiello
authors.forename: R
authors.initials: R
authors.lastname: Mesa-Tejada
authors.forename: J S
authors.initials: JS
authors.lastname: Fang
authors.forename: M B
authors.initials: MB
authors.lastname: Ducayen-Knowles
mesh_terms: D000818:Animals; D002875:Chromosomes / Q000737:chemistry; D006224:Cricetinae; D004247:DNA / Q000378:metabolism*; D007150:Immunohistochemistry; D008297:Male; D008540:Meiosis; D008647:Mesocricetus; D008677:Metaphase; D013087:Spermatids / Q000737:chemistry; D013093:Spermatogonia / Q000737:chemistry; D013737:Testis / Q000254:growth & development* / Q000378:metabolism
chemical_list: D004247:DNA