Antioxidant system of Litomosoides carinii and Setaria cervi: effect of a macrofilaricidal agent.
Filarial parasites, Litomosoides carinii and Setaria cervi, showed great susceptibility to the oxidants generated in vitro by the xanthine/xanthine-oxidase system. In order to counteract such injurious effects, both the filariids possessed an active antioxidant enzymes system. Superoxide dismutase, catalase and glutathione peroxidase were detected in appreciable amounts but glutathione reductase and glucose-6-phosphate dehydrogenase in very low quantities. The former three enzymes were also found to be released by the parasites into the ambient medium. The released enzymes may be responsible for scavenging the host-generated oxidants present in the immediate surroundings of the parasites and thereby enabling them to live comfortably in the host. This Institute-based antifilarial agent namely Compound 82/437 which is 2,2'-dicarbomethoxylamino-5,5'-dibenzimidazolylketone, markedly inhibited catalase and glutathione peroxidase of both L. carinii and S. cervi. The compound, therefore, appears to render the filariids prone to H2O2 toxicity leading to penultimate damage.
Batra
S
S
Division of Biochemistry, Central Drug Research Institute, Lucknow, India.
Chatterjee
R K
RK
Srivastava
V M
VM
D000818:Animals; D001562:Benzimidazoles / Q000494:pharmacology*; D002374:Catalase / Q000032:analysis; D005369:Filaricides / Q000494:pharmacology*; D005370:Filarioidea / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D005954:Glucosephosphate Dehydrogenase / Q000032:analysis; D005979:Glutathione Peroxidase / Q000032:analysis; D005980:Glutathione Reductase / Q000032:analysis; D010084:Oxidation-Reduction; D012719:Setariasis / Q000469:parasitology; D013482:Superoxide Dismutase / Q000032:analysis
D001562:Benzimidazoles; D005369:Filaricides; C062733:2,2'-dicarbomethoxyamino-5-5'-dibenzimidazolyl ketone; D005954:Glucosephosphate Dehydrogenase; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005980:Glutathione Reductase