Characterization of the Syrian hamster c-Ha-ras gene and intron-D-exon transcript.
The coding sequences as well as 5'- and 3'-flanking sequences of the Syrian hamster c-Ha-ras gene were deduced from cDNA clones derived from embryo fibroblast cell lines. Sequences of introns B, C, and D were obtained from genomic DNA after amplification by the polymerase chain reaction. Sequence comparisons with rat, mouse, and human c-Ha-ras genes revealed a high degree of homology. One of 12 cDNA clones contained intron-D-exon (IDX) sequences due to alternative splicing that would encode a p19 Ha-ras gene product. Conservation between species suggests a functional role for the IDX, possibly as a negative control of p21 Ha-ras expression.
Ebert
R
R
Laboratory of Molecular Carcinogenesis, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709.
Wiseman
R W
RW
Barrett
J C
JC
Reiss
E
E
Rollich
G
G
Schiffmann
D
D
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002461:Cell Line, Transformed; D003001:Cloning, Molecular; D006224:Cricetinae; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D005091:Exons; D011905:Genes, ras; D006801:Humans; D007438:Introns; D008647:Mesocricetus; D008969:Molecular Sequence Data; D016283:Proto-Oncogene Proteins p21(ras) / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic
D004247:DNA; C501009:HRAS protein, human; D016283:Proto-Oncogene Proteins p21(ras)