[Influence of heavy metal ions on the electrophysical properties of Anacystis nidulans and Escherichia coli cells].
The influence of heavy metal ions (Ag+, Cu2+, Cd2+, Pb2+, Mn2+, Zn2+, Gd3+, 1 microM-1 mM) on Anacystis nidulans and Escherichia coli cells has been studied by means of electrophoresis and electro-orientation spectroscopy methods. It has been shown that changes of cell electrophoretic mobility (EM) and low-frequency (20 Hz) electro-orientation effect (EOE) observed with the increase of metal cation concentration characterize the adsorption of these ions on surface layers of cell envelopes. The degree and the character of these changes depend on cation valency and the initial value of cell EM. At the same time different changes of EM and EOE as a result of the multivalent cation adsorption allows to conclude that in that case the anisotropy of the cell surface increases. Cell damages were determined by changes in high-frequency EOE of cells which indicated the disturbance of barrier properties of their cytoplasmic membrane. Toxic effects of Ag+, Cu2+, Cd2+ ions on cells of both species and of Pb2+ on E.coli cells were observed. By toxic effects on the cytoplasmic membrane these ions could be placed in the order: for A.nidulans cells--Ag+ greater than Cu2+ greater than Cd2+; for E.coli cells Ag+ greater than Cu2+ greater than Cd2+ greater than Pb2+. Higher toxicity of heavy metals on E.coli cells seems to be connected with the more negative charge of deep layers of the cell surface.
Ivanov
A I
AI
Fomchenkov
V M
VM
Khasanova
L A
LA
Kuramshina
Z M
ZM
Sadikov
M M
MM
D000327:Adsorption; D002412:Cations / Q000493:pharmacokinetics / Q000494:pharmacology; D002462:Cell Membrane / Q000378:metabolism; D000458:Cyanobacteria / Q000187:drug effects* / Q000378:metabolism; D004586:Electrophoresis; D004926:Escherichia coli / Q000187:drug effects* / Q000378:metabolism; D008670:Metals / Q000633:toxicity*; D013057:Spectrum Analysis / Q000379:methods
D002412:Cations; D008670:Metals
Vliianie ionov tiazhelykh metallov na élektrofizicheskie svoĭstva bakterial'nykh kletok Anacystis nidulans i Escherichia coli.