The ability of bovine mammary tissue to synthesize lipids for 96 h when cocultured with liver and adipose tissue.
Explants of bovine mammary, liver, and adipose tissues were cocultured in the same well for 24, 48, 72 or 96 h and their ability to synthesize lipids was measured by the incorporation of [14C]acetate into triglycerides, free fatty acids, and phospholipids. The model was developed to study the effect of bovine somatotropin on lipid synthesis in mammary tissue, because somatotropin will not affect mammary tissue unless liver tissue is present. Somatotropin reduced incorporation by mammary tissue relative to the control. However, the rate of incorporation remained constant through 96 h with somatotropin, but steadily decreased in control mammary tissue. Phospholipid release into the media increased dramatically from 2% of that synthesized during the first 24 h to 218% of that synthesized from 72 to 96 h. The high release of phospholipids after 24 h suggests that cellular breakdown was occurring. A 24-h incubation is recommended to study lipid synthesis with this system.
Keys
J E
JE
Milk Secretion and Mastitis Laboratory, U.S. Department of Agriculture, Beltsville, MD 20705.
Fekry
A E
AE
Wood
D L
DL
Capuco
A V
AV
D000273:Adipose Tissue / Q000378:metabolism* / Q000502:physiology; D000818:Animals; D002417:Cattle; D005260:Female; D013006:Growth Hormone / Q000494:pharmacology; D007334:Insulin-Like Growth Factor I / Q000378:metabolism; D008055:Lipids / Q000096:biosynthesis*; D008099:Liver / Q000378:metabolism*; D008321:Mammary Glands, Animal / Q000378:metabolism*; D008563:Membrane Lipids / Q000378:metabolism; D009924:Organ Culture Techniques; D010743:Phospholipids / Q000378:metabolism; D013696:Temperature; D013997:Time Factors
D008055:Lipids; D008563:Membrane Lipids; D010743:Phospholipids; D007334:Insulin-Like Growth Factor I; D013006:Growth Hormone