HIV infects glomerular endothelial and mesangial but not epithelial cells in vitro.
The infectivity of human immunodeficiency virus (HIV-1) in human glomerular cells was evaluated by exposing homogeneous cultures of human glomerular capillary endothelial, mesangial and epithelial cells to HIV in vitro. Infectivity and HIV expression was assessed by: 1) the measurement of p24 antigen production from culture supernatants; 2) the presence of p24 antigen intracellularly by immunofluorescence; 3) levels of P24 antigen production or syncytia formation following the cocultivation of glomerular cells exposed to HIV with normal human peripheral blood mononuclear cells or MT-2 lymphocytes; and 4) the presence of intracellular HIV DNA by polymerase chain reaction. The results indicate that HIV can infect and replicate in glomerular capillary endothelial cells and in a small percentage of mesangial cells, but not in human glomerular epithelial cells in vitro.
Green
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DF
Division of Nephrology, University of Miami School of Medicine, Florida.
Resnick
L
L
Bourgoignie
J J
JJ
D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000382:microbiology / Q000473:pathology; D004848:Epithelium / Q000378:metabolism / Q000473:pathology; D005920:Glomerular Mesangium / Q000382:microbiology / Q000473:pathology; D006678:HIV / Q000472:pathogenicity / Q000502:physiology; D015658:HIV Infections / Q000382:microbiology* / Q000473:pathology; D006801:Humans; D007674:Kidney Diseases / Q000382:microbiology* / Q000473:pathology; D007678:Kidney Glomerulus / Q000382:microbiology* / Q000473:pathology; D014779:Virus Replication