The effect of pralidoxime chloride in the assay of acetylcholinesterase using 5,5'-dithio-bis(2-nitrobenzoic acid) (Ellman's reagent).
Pralidoxime chloride (PAM) hydrolyzes acetylthiocholine, the substrate used in the assay of red cell cholinesterase. The thiocholine that is produced forms a yellow complex when Ellman's reagent is used in the assay. This was tested in blood samples of patients who were treated with PAM after organophosphorus (OP) poisoning and after the observation of an immediate increase in absorption of light at 412 nm.
Nadarajah
B
B
Institute of Fundamental Studies, Kandy, Sri Lanka.
D000110:Acetylcholinesterase / Q000097:blood*; D000122:Acetylthiocholine / Q000378:metabolism; D002801:Cholinesterase Reactivators / Q000627:therapeutic use; D004228:Dithionitrobenzoic Acid; D004912:Erythrocytes / Q000201:enzymology; D006801:Humans; D007306:Insecticides / Q000506:poisoning; D009943:Organophosphorus Compounds; D011220:Pralidoxime Compounds / Q000737:chemistry* / Q000627:therapeutic use; D013056:Spectrophotometry, Ultraviolet
D002801:Cholinesterase Reactivators; D007306:Insecticides; D009943:Organophosphorus Compounds; D011220:Pralidoxime Compounds; D000122:Acetylthiocholine; D004228:Dithionitrobenzoic Acid; D000110:Acetylcholinesterase; C028797:pralidoxime