title: Signal transduction cascade shared by epidermal growth factor and platelet-derived growth factor is a major pathway for oncogenic transformation in NRK cells.
abstract: We have isolated two recessive, mutually complementary NRK cell mutants that are refractory to transformation by epidermal growth factor (EGF) and transforming growth factor-beta. Both mutants are defective in a signal transduction cascade shared by EGF and platelet-derived growth factor (PDGF). Analysis of the mutants suggests that transformation of NRK cells by the v-fms, v-erbB, activated erbB-2, v-ras, v-fos, v-mos, v-fes, v-src, SV40 large T, polyomavirus middle T, and human papillomavirus type 16 E6,E7 oncogenes is mediated by the EGF/PDGF signal cascade. The data also suggest that the EGF/PDGF cascade branches into mitogenic and oncogenic signals, the latter of which is required for soft agar growth and focus formation.
authors.affiliation: Laboratory of Ultrastructure Research, Aichi Cancer Center Research Institute, Nagoya, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Masuda
authors.forename: S
authors.initials: S
authors.lastname: Kizaka-Kondoh
authors.forename: H
authors.initials: H
authors.lastname: Miwatani
authors.forename: Y
authors.initials: Y
authors.lastname: Terada
authors.forename: H
authors.initials: H
authors.lastname: Nojima
authors.forename: H
authors.initials: H
authors.lastname: Okayama
mesh_terms: D000818:Animals; D002455:Cell Division; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D004815:Epidermal Growth Factor / Q000502:physiology*; D066246:ErbB Receptors / Q000502:physiology; D015870:Gene Expression; D009154:Mutation; D009857:Oncogenes; D010982:Platelet-Derived Growth Factor / Q000502:physiology*; D015398:Signal Transduction / Q000235:genetics / Q000502:physiology; D014162:Transfection
chemical_list: D010982:Platelet-Derived Growth Factor; D004815:Epidermal Growth Factor; D066246:ErbB Receptors