Induction of motor neuron sprouting in vivo by ciliary neurotrophic factor and basic fibroblast growth factor.
Ciliary neurotrophic factor (CNTF) and basic fibroblast growth factor (bFGF) were tested for effects on sprouting by motor neurons innervating the adult mouse gluteus muscle. Factors were delivered by subcutaneous injection directly over the surface of the superior gluteus muscle once daily for 7 d and then end plates and axons were visualized by combined silver and cholinesterase staining. CNTF (500 ng daily) induced sprouting both from end plates and from the subset of nodes of Ranvier that are closest to the end plate. The effect of CNTF was potentiated twofold by coadministration of bFGF at doses of 2-20 ng daily, whereas treatment with bFGF alone failed to induce sprouting from either end plates or nodes of Ranvier. The sprouting stimulus delivered by the factors showed limited penetrance into the muscle and restricted lateral spread from the injection site.
Gurney
M E
ME
Department of Cell, Molecular, and Structural Biology, Northwestern University Medical School, Chicago, Illinois 60611.
Yamamoto
H
H
Kwon
Y
Y
D000595:Amino Acid Sequence; D000818:Animals; D001369:Axons / Q000502:physiology; D020934:Ciliary Neurotrophic Factor; D004357:Drug Synergism; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D051379:Mice; D008969:Molecular Sequence Data; D009045:Motor Endplate / Q000502:physiology; D009046:Motor Neurons / Q000187:drug effects / Q000502:physiology*; D009132:Muscles / Q000294:innervation; D009411:Nerve Endings / Q000187:drug effects / Q000502:physiology; D009414:Nerve Growth Factors / Q000494:pharmacology; D009419:Nerve Tissue Proteins / Q000494:pharmacology*; D011901:Ranvier's Nodes / Q000187:drug effects / Q000502:physiology
D020934:Ciliary Neurotrophic Factor; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins; D016222:Fibroblast Growth Factor 2