In vivo changes of heterogeneity of mouse peritoneal macrophages induced by cis-diamminedichloroplatinum (II) (cis-DDP) and related platinum compounds.
The s.c. injection of cis-DDP into ABD2F1 mice (8 mg/kg b.m.) resulted in alterations of size and surface structure of peritoneal macrophages (PM) and in a reduction of the mean number of Concanavalin A (ConA) binding sites of PM. The PM population of control mice which received physiological saline only consisted of 2 subgroups with a higher and a lower mean ConA binding site number per cell. Contrarily, PM of cis-DDP-treated mice failed the subpopulation with higher ConA binding site number. A loss of this subpopulation was also found in mice treated with platinum salt K2PtCl4 or K2PtCl6. X-ray microanalytically determined elemental contents of PM of control and treated mice showed a correlation between ConA binding site number and cellular concentration of phosphorus or sulphur with the exception of a small group of PM which was characterized by a high content of sulphur and a low number of ConA binding site. This correlation was not found in normal mice.
Peschke
T
T
Central Institute of Microbiology and Experimental Therapy, Jena, Germany.
Schröer
H P
HP
Augsten
K
K
D000818:Animals; D001665:Binding Sites; D002945:Cisplatin / Q000008:administration & dosage / Q000494:pharmacology*; D003208:Concanavalin A / Q000378:metabolism; D004577:Electron Probe Microanalysis; D007279:Injections, Subcutaneous; D008264:Macrophages / Q000166:cytology* / Q000378:metabolism / Q000648:ultrastructure; D008297:Male; D051379:Mice; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D010529:Peritoneal Cavity / Q000166:cytology*; D010783:Photometry; D010984:Platinum / Q000008:administration & dosage / Q000494:pharmacology*
D003208:Concanavalin A; D010984:Platinum; D002945:Cisplatin