CD4-class II major histocompatibility complex interaction does not enhance killing by a class I-restricted CD4+CD8+ cytotoxic T cell clone.
As unusual tumor-specific cytotoxic T lymphocyte (CTL) clone was isolated which expressed both CD4 and CD8 molecules. The target cells for this CTL can be induced to express either class I major histocompatibility complex (MHC) alone (with dimethylsulfoxide) or both class I and class II MHC (with interferon-gamma). Lysis of the tumor target depends on expression of class I MHC molecules, but does not require expression of class II MHC molecules. Furthermore, the lysis of target cells expressing both class I and class II is inhibited only by antibodies to class I (Kd), and not by antibodies to class II, demonstrating that the T cell receptor is class I restricted. We have used this CTL to assess the role of the interaction between CD4 and class II MHC in the absence of a class II-restricted T cell receptor. Our data indicate that CD4-class II interaction does not contribute to recognition by T cells in the absence of binding of the T cell receptor to class II molecules.
McAdam
A J
AJ
Division of Immunology, University of Rochester Medical Center, NY.
Blieden
T M
TM
Frelinger
J G
JG
Lord
E M
EM
D000818:Animals; D015704:CD4 Antigens / Q000032:analysis* / Q000502:physiology; D016827:CD8 Antigens / Q000032:analysis*; D002999:Clone Cells; D015395:Histocompatibility Antigens Class I / Q000032:analysis / Q000502:physiology*; D000949:Histocompatibility Antigens Class II / Q000032:analysis / Q000502:physiology*; D051379:Mice; D008815:Mice, Inbred Strains; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
D015704:CD4 Antigens; D016827:CD8 Antigens; D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II