Effect of chronic pre- and post-natal low-dose ethanol exposure on brain enolase isoenzyme activities.
Sprague-Dawley dams were treated with 3 v/v % ethanol in liquid diet from the 8th day gestation through 3 weeks nursing period. Offsprings, ages 1, 2 and 3 weeks, and their dams were studied. Brain weights and their total proteins were not affected by this ethanol treatment. Total enolase activity/mg protein and its isoenzymes, i.e. non-neuron-specific, hybrid and neuron-specific, (mumol/min/mg protein) in 100,000 g supernate, were significantly lower in the treated. Additionally, enolase isoenzyme transformation was delayed.
Harada
K
K
Department of Pharmacology, Cornell University Medical College, New York, NY 10021.
Adams
V
V
Okamoto
M
M
D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000201:enzymology; D000431:Ethanol / Q000008:administration & dosage*; D005260:Female; D007774:Lactation / Q000378:metabolism*; D008431:Maternal-Fetal Exchange / Q000502:physiology*; D009419:Nerve Tissue Proteins / Q000378:metabolism; D010751:Phosphopyruvate Hydratase / Q000378:metabolism*; D011247:Pregnancy; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors
D009419:Nerve Tissue Proteins; D000431:Ethanol; D010751:Phosphopyruvate Hydratase