Fragmentation studies of peptides: the formation of y ions.
The mechanism for the formation of y ions in the collision-induced dissociation (CID) spectra of protonated peptides produced by fast-atom bombardment was investigated by tandem mass spectrometry and deuterium labelling studies. The results show that a hydrogen atom attached to nitrogen and not to carbon migrates during cleavage of the amide bond. A mechanism based on these results is presented.
Kenny
P T
PT
Suntory Institute of Bioorganic Research, Osaka, Japan.
Nomoto
K
K
Orlando
R
R
D000595:Amino Acid Sequence; D004187:Disaccharides / Q000235:genetics; D007477:Ions; D008969:Molecular Sequence Data; D010455:Peptides / Q000235:genetics*; D016339:Spectrometry, Mass, Fast Atom Bombardment; D014508:Urea / Q000031:analogs & derivatives
D004187:Disaccharides; D007477:Ions; D010455:Peptides; C073644:mannosyl-(1-4)-N-acetylglucosaminyl-(1-N)-urea; D014508:Urea