title: Further consideration of the clonal nature of Salmonella typhi: evaluation of molecular and clinical characteristics of strains from Indonesia and Peru.
abstract: We examined envelope protein profiles, chromosomal restriction endonuclease digest patterns, and immune responses to envelope proteins for collections of Salmonella typhi strains isolated in Peru and Indonesia. Only minor differences in envelope protein patterns were apparent among strains. Strains from 7 of 20 Indonesian patients had a distinct chromosomal digest pattern compared with patterns of Peruvian and other Indonesian strains. Strains with this pattern carried the gene for the j flagellar antigen (H1-j); differences in response to envelope proteins of j and d strains were noted on immunoblot analysis. Our data suggest that there are genotypic and phenotypic differences among S. typhi strains. The clinical importance of these differences remains to be fully evaluated; however, in this study it was not possible to show a clear correlation between strain characteristics and disease severity.
authors.affiliation: Department of Medicine, University of Maryland School of Medicine, Baltimore 21201.
authors.forename: A
authors.initials: A
authors.lastname: Franco
authors.forename: C
authors.initials: C
authors.lastname: Gonzalez
authors.forename: O S
authors.initials: OS
authors.lastname: Levine
authors.forename: R
authors.initials: R
authors.lastname: Lagos
authors.forename: R H
authors.initials: RH
authors.lastname: Hall
authors.forename: S L
authors.initials: SL
authors.lastname: Hoffman
authors.forename: M A
authors.initials: MA
authors.lastname: Moechtar
authors.forename: E
authors.initials: E
authors.lastname: Gotuzzo
authors.forename: M M
authors.initials: MM
authors.lastname: Levine
authors.forename: D M
authors.initials: DM
authors.lastname: Hone
mesh_terms: D001426:Bacterial Proteins / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000235:genetics; D005407:Flagella; D005798:Genes, Bacterial; D006801:Humans; D015151:Immunoblotting; D007214:Indonesia; D010568:Peru; D016133:Polymerase Chain Reaction; D012485:Salmonella typhi / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D014435:Typhoid Fever / Q000276:immunology / Q000382:microbiology
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial