In vitro production of interleukin-1 from blood mononuclear cells of patients on chronic hemodialysis therapy.
A monocyte defect is thought to be involved in the impaired immune response in patients on regular hemodialysis therapy. As an indicator of cell function, we studied in vitro IL-1 beta production of mononuclear cells from hemodialysis patients in comparison to normal controls. Mononuclear cells were stimulated with endotoxin or Staphylococcus epidermidis in parallel with control incubations in tissue culture medium alone. Spontaneous as well as stimulated total IL-1 beta production (cell-associated plus extracellular) did not differ significantly in cells obtained from patients compared to those from normal controls. However, the relative amounts of IL-1 beta released into the cell supernatants were significantly reduced in mononuclear cells from hemodialysis patients when stimulated with endotoxin but not with Staphylococcus epidermidis. These data indicate a stimulus-dependent defect in the mechanism of IL-1 beta release. As IL-1 is necessary for T-cell activation this alteration in mononuclear cell function may play a role in the impaired cellular immunity observed in patients on chronic hemodialysis therapy.
Haubitz
M
M
Department of Nephrology, University Hospital Hannover, Germany.
Klöppel
B
B
Lonnemann
G
G
Nonnast-Daniel
B
B
D003470:Culture Media; D004731:Endotoxins / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D007375:Interleukin-1 / Q000096:biosynthesis*; D007676:Kidney Failure, Chronic / Q000276:immunology* / Q000628:therapy; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000378:metabolism*; D011863:Radioimmunoassay; D006435:Renal Dialysis; D013212:Staphylococcus epidermidis
D003470:Culture Media; D004731:Endotoxins; D007375:Interleukin-1