Complementary physical and genetic techniques map the vinculin (VCL) gene on chromosome 10q.
Vinculin is a cytoskeletal protein component of adherens type cell junctions. The gene had been mapped to 10q11.2-qter. We have used a combination of physical and genetic mapping techniques to refine this localization. Hybridization of the vinculin cDNA probe, HV1, to a human-rodent somatic hybrid panel initially suggested a position of either 10q11.2 or 10q22.1-10q23. Genetic recombination mapping in three-generation families with multiple endocrine neoplasia type 2 (MEN2) indicated a position distal to D10S22 (10q21.1) in 10q22.1-10q23. This was confirmed by hybridization of the vinculin cDNA to flow-sorted translocation derivative chromosomes containing the q21-qter portion of chromosome 10. We conclude that the vinculin locus maps in 10q22.1-q23, distal to D10S22.
Mulligan
L M
LM
Department of Pathology, University of Cambridge, United Kingdom.
Gardner
E
E
Telenius
H
H
Ponder
B A
BA
D000818:Animals; D002871:Chromosome Banding; D002874:Chromosome Mapping; D002879:Chromosomes, Human, Pair 10; D006224:Cricetinae; D004247:DNA / Q000235:genetics; D015342:DNA Probes; D006801:Humans; D006822:Hybrid Cells; D008677:Metaphase; D051379:Mice; D016596:Vinculin / Q000235:genetics*
D015342:DNA Probes; D016596:Vinculin; D004247:DNA