Denaturation of human Cu/Zn superoxide dismutase by guanidine hydrochloride: a dynamic fluorescence study.
The unfolding of holo and apo forms of human Cu/Zn superoxide dismutase by guanidine hydrochloride has been investigated by steady-state and dynamic fluorescence. In agreement with previous observations, a stabilizing effect of the metal ions on the protein tertiary structure was apparent from comparison of apo- and holoproteins, which both showed a sharp sigmoidal transition though at different denaturant concentrations. The transition was also followed by circular dichroism to check the extent of secondary structure present at each denaturant concentration. The results are incompatible with a simple two-state mechanism for denaturation. The occurrence of a more complicated process is supported by the emission decay properties of the single tryptophanyl residue at different denaturant concentrations. A complex decay function, namely, two discrete exponentials or a continuous distribution of lifetimes, was always required to fit the data. In particular, the width of the lifetime distribution, which is maximum at the transition midpoint, reflects heterogeneity of the tryptophan microenvironment and thus the presence of different species along the denaturation pathway. In the unfolded state, the width of the lifetime distribution is broader than in the folded state probably because the tryptophan residue is affected by a larger number of local conformations. The dissociation of the dimer was also studied by varying the protein concentration at different denaturant concentrations. This process affects primarly the surface of the protein rather than its secondary structure as shown by a comparison between the tryptophan emission decay and circular dichroism data under the same conditions. Another consequence of dissociation is a greater instability in the structure of the monomers, which are more easily unfolded.(ABSTRACT TRUNCATED AT 250 WORDS)
Mei
G
G
Dipartimento di Medicina Sperimentale e Scienze Biochimiche, Università di Roma, Tor Vergata, Italy.
Rosato
N
N
Silva
N
N
Rusch
R
R
Gratton
E
E
Savini
I
I
Finazzi-Agrò
A
A
D001051:Apoenzymes / Q000097:blood / Q000737:chemistry; D002942:Circular Dichroism; D004912:Erythrocytes / Q000201:enzymology; D005454:Fluorescence Polarization; D019791:Guanidine; D006146:Guanidines / Q000494:pharmacology*; D006801:Humans; D007700:Kinetics; D011487:Protein Conformation; D011489:Protein Denaturation; D013050:Spectrometry, Fluorescence; D013482:Superoxide Dismutase / Q000037:antagonists & inhibitors / Q000097:blood* / Q000737:chemistry
D001051:Apoenzymes; D006146:Guanidines; D013482:Superoxide Dismutase; D019791:Guanidine