title: Alpha satellite DNAs on chromosomes 10 and 12 are both members of the dimeric suprachromosomal subfamily, but display little identity at the nucleotide sequence level.
abstract: We have investigated the organization and complexity of alpha satellite DNA on chromosomes 10 and 12 by restriction endonuclease mapping, in situ hybridization (ISH), and DNA-sequencing methods. Alpha satellite DNA on both chromosomes displays a basic dimeric organization, revealed as a 6- and an 8-mer higher-order repeat (HOR) unit on chromosome 10 and as an 8-mer HOR on chromosome 12. While these HORs show complete chromosome specificity under high-stringency ISH conditions, they recognize an identical set of chromosomes under lower stringencies. At the nucleotide sequence level, both chromosome 10 HORs are 50% identical to the HOR on chromosome 12 and to all other alpha satellite DNA sequences from the in situ cross-hybridizing chromosomes, with the exception of chromosome 6. An 80% identity between chromosome 6- and chromosome 10-derived alphoid sequences was observed. These data suggest that the alphoid DNA on chromosomes 6 and 10 may represent a distinct subclass of the dimeric subfamily. These sequences are proposed to be present, along with the more typical dimeric alpha satellite sequences, on a number of different human chromosomes.
authors.affiliation: Laboratory of Experimental Patho-Oncology, Dr. Daniel den Hoed Cancer Center, Rotterdam, The Netherlands.
authors.forename: L H
authors.initials: LH
authors.lastname: Looijenga
authors.forename: J W
authors.initials: JW
authors.lastname: Oosterhuis
authors.forename: V T
authors.initials: VT
authors.lastname: Smit
authors.forename: J W
authors.initials: JW
authors.lastname: Wessels
authors.forename: P
authors.initials: P
authors.lastname: Mollevanger
authors.forename: P
authors.initials: P
authors.lastname: Devilee
mesh_terms: D001483:Base Sequence; D015139:Blotting, Southern; D002879:Chromosomes, Human, Pair 10; D002881:Chromosomes, Human, Pair 12; D004276:DNA, Satellite / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D012091:Repetitive Sequences, Nucleic Acid; D012689:Sequence Homology, Nucleic Acid
chemical_list: D004276:DNA, Satellite