title: Delimitation of cohesive ends site (cos) of Streptomyces temperate bacteriophage R4.
abstract: The cohesive ends site (cos) of actinophage R4 was delimitated by assaying the in vivo packaging activity of cosmid derivatives in Streptomyces lividans. A region of 66 bp from -30 to +36 from the center of cohesive ends was required for the basal level of packaging activity. Two additional regions outside the basal sequences from -39 to -31 and from +37 to +97 were necessary for the high level of activity, defined as the accessory sequences. Direct- or inverted-repeat sequences were found within the delimitated region, which might be involved in the recognition by the terminase of actinophage R4.
authors.affiliation: Institute of Applied Microbiology, University of Tokyo.
authors.forename: H
authors.initials: H
authors.lastname: Mitsui
authors.forename: H
authors.initials: H
authors.lastname: Takahashi
mesh_terms: D001435:Bacteriophages / Q000235:genetics*; D001482:Base Composition; D001483:Base Sequence; D001665:Binding Sites; D003360:Cosmids; D004274:DNA, Recombinant / Q000378:metabolism; D004279:DNA, Viral / Q000235:genetics*; D004706:Endodeoxyribonucleases / Q000378:metabolism; D008969:Molecular Sequence Data; D010957:Plasmids; D015183:Restriction Mapping; D013302:Streptomyces / Q000235:genetics*; D014161:Transduction, Genetic
chemical_list: D004274:DNA, Recombinant; D004279:DNA, Viral; D004706:Endodeoxyribonucleases; C030894:terminase