title: Pulmonary artery occlusion is sufficient to increase pulmonary vascular permeability in rabbits.
abstract: Unilateral pulmonary artery obstruction (PAO) for 24-48 h, followed by reperfusion, results in pulmonary edema and lung inflammation. We hypothesized that lung injury actually occurred during the period of PAO but, because of low microvascular pressures during the period of occlusion, was not detected until perfusion was reestablished. To test this hypothesis, we studied 14 rabbits divided into three groups: group I rabbits underwent sham occlusion of the left pulmonary artery for 24 h; group II rabbits underwent PAO but were not reperfused; and group III rabbits were subjected to PAO and then reperfused for 4 h. The fluid filtration coefficient measured during a zone 3 no-flow hydrostatic stress (pulmonary arterial pressure = pulmonary venous pressure, both greater than alveolar pressure) in group I lungs was less than that of lungs in either group II or III [0.52 +/- 0.02 (SE) ml.min-1.cmH2O.100 g wet wt-1 vs. 0.94 +/- 0.11 and 0.86 +/- 0.13 for groups II and III, respectively, P less than 0.05]. The wet-to-dry weight ratio of the left lung measured after the zone 3 stress was applied for 20 min was 6.90 +/- 0.09 in group I rabbits and 9.21 +/- 0.75 and 11.75 +/- 0.44 in groups II and III, respectively (P less than 0.05). Radiolabeled microspheres demonstrated that flow to the left lung was diminished after the period of PAO (38 +/- 4, 9 +/- 5, and 2 +/- 1% of cardiac output in groups I, II, and III, respectively; P less than 0.05 for group I vs. groups II and III).(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Anesthesiology, Department of Veterans Affairs Medical Service, Seattle, Washington.
authors.forename: M J
authors.initials: MJ
authors.lastname: Bishop
authors.forename: W
authors.initials: W
authors.lastname: Lamm
authors.forename: S M
authors.initials: SM
authors.lastname: Guidotti
authors.forename: R K
authors.initials: RK
authors.lastname: Albert
mesh_terms: D000818:Animals; D001157:Arterial Occlusive Diseases / Q000503:physiopathology*; D002199:Capillary Permeability / Q000502:physiology*; D008863:Microspheres; D009929:Organ Size / Q000502:physiology; D011651:Pulmonary Artery / Q000503:physiopathology*; D011654:Pulmonary Edema / Q000503:physiopathology; D011817:Rabbits; D015424:Reperfusion