Use of an ATP-supplemented medium for the conservation of human semen and the effect of caffeine on the motility of preserved sperm. Results in artificial insemination.
Human semen can be preserved at 4 degrees C in an artificial medium containing 15% ATP-MgCl2 for up to 5 d. This method has been applied to samples from subjects with either normal or abnormal semen. After the conservation period the recovery of sperm motility observed was good when low doses of caffeine (3.5 or 7.0 mM) were added.
Diaz
J W
JW
Hospital Gineco-Obstetrico Ramón Gonzalez Coro, Habana, Cuba.
Gonzalez
M A
MA
Avedo
F
F
Mallea
L
L
Rodriguez
N
N
D000255:Adenosine Triphosphate; D002110:Caffeine / Q000494:pharmacology; D003080:Cold Temperature; D003470:Culture Media; D006801:Humans; D066298:In Vitro Techniques; D007315:Insemination, Artificial; D008297:Male; D011309:Preservation, Biological / Q000379:methods*; D012661:Semen; D013081:Sperm Motility / Q000187:drug effects
D003470:Culture Media; D002110:Caffeine; D000255:Adenosine Triphosphate