Heterogeneity of carboxylesterases in rat liver cells.
Rat liver cells were separated into parenchymal cells (PC), Kupffer cells (KC) and endothelial cells (EC). The distribution of carboxylesterases (EC 3.1.1.1) between these cell types was investigated by PAGE and chromatogenic substrate staining, and compared with the results for total liver preparation and individual isoenzymes isolated by chromatofocusing. All of the liver carboxylesterase isoenzymes could be detected in the PC, whereas in both KC and EC only those with isoelectric point (pI) 6.4/6.2 could be detected. Use of carboxylesterase inhibitors like bis-(4-nitrophenyl)phosphate and paraoxon, and organophosphorus compound hydrolase inhibitors like 4-hydroxymercuribenzoate and EDTA confirmed that these esterases were of the carboxylesterase type.
Gaustad
R
R
Norwegian Defence Research Establishment, Division for Environmental Toxicology, Kjeller, Norway.
Berg
T
T
Fonnum
F
F
D000818:Animals; D002265:Carboxylic Ester Hydrolases / Q000032:analysis* / Q000037:antagonists & inhibitors; D004591:Electrophoresis, Polyacrylamide Gel; D004727:Endothelium / Q000201:enzymology; D007525:Isoelectric Focusing; D007527:Isoenzymes / Q000032:analysis* / Q000037:antagonists & inhibitors; D007728:Kupffer Cells / Q000201:enzymology; D008099:Liver / Q000201:enzymology*; D008297:Male; D008994:Monoacylglycerol Lipases / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains
D007527:Isoenzymes; D002265:Carboxylic Ester Hydrolases; D008994:Monoacylglycerol Lipases