A cleavable biotinylating agent and its use in protein electroblots.
A direct method for the synthesis of N-biotinyl penicillamine is described. It has been shown to be a convenient biotinylating agent for antibodies which have been previously coupled with SPDP. The biotinylated antibodies can be used to detect antigens on protein electroblots using 125I-labelled streptavidin and radioautography. The biotin and its attached streptavidin and radiolabel can be removed under mild conditions and the blot reprobed with a different antibody using an identical protocol.
Hobart
M J
MJ
Molecular Immunopathology Unit, M.R.C. Centre, Cambridge, UK.
Fernie
B A
BA
Lachmann
P J
PJ
D000911:Antibodies, Monoclonal / Q000276:immunology; D001345:Autoradiography; D001710:Biotin; D010396:Penicillamine; D011506:Proteins / Q000032:analysis*
D000911:Antibodies, Monoclonal; D011506:Proteins; D001710:Biotin; D010396:Penicillamine