Molecular analysis of simian varicella virus DNA.
Clinical and pathological studies indicate that simian varicella virus (SVV) infection in primates is the counterpart of human varicella zoster virus (VZV) infection. The SVV and VZV genomes are also similar in size and structure. To extend studies of SVV DNA, we analyzed virus DNA from African green monkey kidney cells infected with the Delta-herpes-virus strain of SVV. The infectivity of SVV DNA was 88 PFU/micrograms. The buoyant density of SVV DNA, determined by isopycnic banding in CsCl gradients, was 1.700 +/- 0.002 g/ml, corresponding to a G + C molar ratio of 40.8%. The size of SVV DNA, estimated by analysis of restriction endonuclease digestion products and pulsed-field gel electrophoresis was 125.1 and 124.9 kbp, respectively. Electron microscopy of SVV DNA revealed a long region of 110.0 kbp, a unique short (Us) region of 5.1 kbp, and inverted repeat regions of 7.5 kbp flanking the Us. An EcoRI map of SVV DNA revealed two fragments not previously reported; our complete Pstl map also shows some differences. Mapping of SVV DNA with an additional restriction enzyme, measurement of full-length SVV DNA molecules, and the first use of pulsed-field electrophoresis to size SVV DNA, confirm and extend Gray's recent finding that SVV DNA has the same size and molecular configuration as VZV. We also show for the first time that the density of SVV DNA is similar to that of VZV DNA and that SVV DNA is infectious.
Clarke
P
P
Department of Neurology, University of Colorado School of Medicine, Denver 80262.
Rabkin
S D
SD
Inman
M V
MV
Mahalingam
R
R
Cohrs
R
R
Wellish
M
M
Gilden
D H
DH
D000818:Animals; D001482:Base Composition / Q000235:genetics; D002460:Cell Line; D002522:Chlorocebus aethiops; D004279:DNA, Viral / Q000235:genetics* / Q000648:ultrastructure; D016521:Electrophoresis, Gel, Pulsed-Field; D000882:Haplorhini / Q000382:microbiology*; D006564:Herpesviridae / Q000235:genetics*; D008854:Microscopy, Electron; D015183:Restriction Mapping; D014162:Transfection / Q000235:genetics; D010948:Viral Plaque Assay
D004279:DNA, Viral