Characterization and uses of monoclonal antibody derived against DNA binding domain of the ets family of genes.
A monoclonal antibody recognizing ets proteins from a variety of species has been developed. This antibody recognizes ets1, ets2, erg, and other related proteins. It has a high affinity for the ets1 protein. The epitope for the pan ets mAb consists of about 13 amino acids. This antibody can be used to isolate and characterize new members of ets gene family derived from a c-DNA expression library, as well as to identify other "ets motif" binding proteins.
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Laboratory of Molecular Oncology, National Cancer Institute, Frederick, MD.
Papas
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D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D000941:Antigens / Q000235:genetics / Q000276:immunology; D001483:Base Sequence; D001665:Binding Sites; D004247:DNA / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics / Q000276:immunology; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D008969:Molecular Sequence Data; D005810:Multigene Family; D010455:Peptides / Q000235:genetics / Q000276:immunology; D015682:Retroviridae Proteins, Oncogenic / Q000235:genetics* / Q000276:immunology*; D013045:Species Specificity
D000911:Antibodies, Monoclonal; D000941:Antigens; D004268:DNA-Binding Proteins; D010455:Peptides; D015682:Retroviridae Proteins, Oncogenic; C050379:oncogene proteins v-ets; D004247:DNA