DNA extraction from mixtures of body fluid using mild preferential lysis.
A modification to the DNA extraction method "preferential lysis" (Gill et al. 1985) is proposed which can be applied to DNA mixtures of vaginal cells and spermatozoa. In mixtures with a low sperm content the further loss of sperm DNA caused by the extraction can be avoided by using "mild preferential lysis". Amplification by PCR (polymerase chain reaction) then yields sufficient DNA to be able to identify both components in the mixture.
Wiegand
P
P
Institut für Rechtsmedizin, Westfälische Wilhelms-Universität Münster, Federal Republic of Germany.
Schürenkamp
M
M
Schütte
U
U
D001826:Body Fluids / Q000737:chemistry*; D002582:Cervix Mucus / Q000737:chemistry; D004247:DNA / Q000032:analysis* / Q000235:genetics; D005260:Female; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods; D014626:Vaginal Smears
D004247:DNA