In vivo and in vitro growth-inhibitory effect of bovine seminal ribonuclease on a system of rat thyroid epithelial transformed cells and tumors.
We investigated the antitumoral effect of bovine seminal RNase (BS-RNase) in vivo and in vitro on a model system of epithelial tumor- and metastasis-derived cells as well as on epithelial tumors derived from the same system. We found that while BS-RNase significantly inhibited the growth in vitro of the epithelial tumor-derived cells, its inhibitory effect was even more dramatic on the growth of metastasis-derived cells. BS-RNase exerted no appreciable growth inhibition on normal thyroid epithelial cells. When administered in vivo to rats bearing solid carcinomas, having the same thyroid origin, BS-RNase induced a drastic reduction in the tumor weight, with no detectable toxic effects on the treated animals. These data show, for the first time on a system of neoplastically transformed epithelial cells, that BS-RNase has a potent specific antitumoral activity.
Laccetti
P
P
Dipartimento di Medicina Sperimentale e Clinica, Università degli Studi di Reggio Calabria, Facoltà di Medicina e Chirurgia di Catanzaro, Italy.
Portella
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G
Mastronicola
M R
MR
Russo
A
A
Piccoli
R
R
D'Alessio
G
G
Vecchio
G
G
D000818:Animals; D000970:Antineoplastic Agents; D002417:Cattle; D002454:Cell Differentiation; D002455:Cell Division / Q000187:drug effects*; D002472:Cell Transformation, Viral; D004305:Dose-Response Relationship, Drug; D004848:Epithelium / Q000473:pathology; D009362:Neoplasm Metastasis; D009374:Neoplasms, Experimental / Q000473:pathology*; D051381:Rats; D012260:Ribonucleases / Q000494:pharmacology*; D012661:Semen / Q000201:enzymology; D013961:Thyroid Gland / Q000166:cytology; D013964:Thyroid Neoplasms / Q000473:pathology; D014407:Tumor Cells, Cultured / Q000187:drug effects*
D000970:Antineoplastic Agents; D012260:Ribonucleases