title: Molecular characterization of HIV-2 (ROD) protease following PCR cloning from virus infected H9 cells.
abstract: A 450 nucleotide sequence corresponding to the nucleotides 1931-2380 of the viral genome (8) was amplified by polymerase chain reaction (PCR) using template DNA prepared from HIV-2 (ROD) infected H9 cells. The sequence codes for HIV-2 protease and its N-terminal flanking peptide. An identical DNA sequence was obtained from three independent PCR amplifications, which differs from the published sequence of HIV-2 (ROD) in 7 nucleotides scattered throughout the region of the cloned DNA. The cloned DNA was expressed in E. coli cells and resulted in the synthesis of a correctly processed HIV-2 protease, which is enzymatically active. Therefore, none of the seven nucleotide changes, which resulted in two amino acid substitutions, affect the autoproteolytic or trans-cleaving activities of the HIV-2 protease.
authors.affiliation: DuPont Merck Pharmaceutical Company, Wilmington, DE 19880-0328.
authors.forename: Y S
authors.initials: YS
authors.lastname: Cheng
authors.forename: C E
authors.initials: CE
authors.lastname: Patterson
authors.forename: R G
authors.initials: RG
authors.lastname: Rucker
authors.forename: M J
authors.initials: MJ
authors.lastname: Otto
authors.forename: C J
authors.initials: CJ
authors.lastname: Rizzo
authors.forename: B D
authors.initials: BD
authors.lastname: Korant
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000737:chemistry; D004789:Enzyme Activation; D015658:HIV Infections / Q000235:genetics* / Q000382:microbiology; D016333:HIV Protease / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification; D015498:HIV-2 / Q000201:enzymology* / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction
chemical_list: D004279:DNA, Viral; D016333:HIV Protease