title: A simple enhancement method for the silver-gold-intensified diaminobenzidine reaction in the light microscopic immunoperoxidase technique.
abstract: We describe a simple and sensitive method for enhancement of the silver-gold-intensified 3,3'-diaminobenzidine (DAB) reaction demonstrating peroxidase activity. After completing silver-gold intensification of the preparations immunostained by the avidin-biotin-peroxidase method with DAB as the chromogen, the preparations were immersed in a solution containing uranyl nitrate. This new method appeared to increase the sensitivity by at least one order of magnitude as compared with silver-gold intensification alone.
authors.affiliation: Department of Neurosurgery, Kumamoto University Medical School, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Goto
authors.forename: S
authors.initials: S
authors.lastname: Nagahiro
authors.forename: Y
authors.initials: Y
authors.lastname: Ushio
authors.forename: W
authors.initials: W
authors.lastname: Hofer
mesh_terms: D015100:3,3'-Diaminobenzidine / Q000737:chemistry*; D000818:Animals; D001360:Avidin; D001710:Biotin; D006046:Gold; D007124:Immunoenzyme Techniques; D010215:Papio; D016622:Silver Staining
chemical_list: D001360:Avidin; D015100:3,3'-Diaminobenzidine; D001710:Biotin; D006046:Gold