Density-dependent responsiveness to autocrine growth factors of Epstein-Barr virus transformed human B lymphocytes.
Analysis of the growth requirements of Epstein-Barr virus (EBV)-transformed B lymphocytes shows that interleukin 1 and thioredoxin, a disulfide reducing enzyme, are able to induce a marked increase in DNA synthesis in the early phases of in vitro culture. By contrast, interleukin 6 induces a steady increase in DNA synthesis comparable to that observed with crude conditioned supernatant. Furthermore, EBV-transformed B cells exhibit a density-dependent responsiveness to autocrine growth factors, thus suggesting that growth regulation of EBV-transformed B cells might result from the interplay between different self-stimulating soluble factors and from the competence of the cells to respond to autocrine growth factors.
Garzelli
C
C
Department of Biomedicine, University of Pisa, Italy.
Bazzichi
A
A
Dayah
A M
AM
Manunta
M
M
Incaprera
M
M
D001402:B-Lymphocytes / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D002455:Cell Division / Q000187:drug effects; D002461:Cell Line, Transformed; D002471:Cell Transformation, Neoplastic; D004247:DNA / Q000096:biosynthesis; D006133:Growth Substances / Q000494:pharmacology*; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology; D015850:Interleukin-6 / Q000494:pharmacology; D007378:Interleukins / Q000494:pharmacology*; D013879:Thioredoxins / Q000494:pharmacology*
D006133:Growth Substances; D007375:Interleukin-1; D015850:Interleukin-6; D007378:Interleukins; D013879:Thioredoxins; D004247:DNA