Tissue- and site-specific DNA recombination in transgenic mice.
We have developed a method of specifically modifying the mammalian genome in vivo. This procedure comprises heritable tissue-specific and site-specific DNA recombination as a function of recombinase expression in transgenic mice. Transgenes encoding the bacteriophage P1 Cre recombinase and the loxP-flanked beta-galactosidase gene were used to generate transgenic mice. Genomic DNA from doubly transgenic mice exhibited tissue-specific DNA recombination as a result of Cre expression. Further characterization revealed that this process was highly efficient at distinct chromosomal integration sites. These studies also imply that Cre-mediated recombination provides a heritable marker for mitoses following the loss of Cre expression. This transgene-recombination system permits unique approaches to in vivo studies of gene function within experimentally defined spatial and temporal boundaries.
Orban
P C
PC
Biomedical Research Centre, University of British Columbia, Vancouver, Canada.
Chui
D
D
Marth
J D
JD
D000818:Animals; D015139:Blotting, Southern; D015704:CD4 Antigens / Q000235:genetics; D016827:CD8 Antigens / Q000235:genetics; D003090:Coliphages / Q000201:enzymology / Q000235:genetics; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D004254:DNA Nucleotidyltransferases / Q000235:genetics* / Q000378:metabolism; D015342:DNA Probes; D013006:Growth Hormone / Q000235:genetics; D006801:Humans; D019426:Integrases; D051379:Mice; D008822:Mice, Transgenic / Q000235:genetics*; D009928:Organ Specificity; D011995:Recombination, Genetic; D015183:Restriction Mapping; D013601:T-Lymphocytes / Q000276:immunology / Q000502:physiology; D014158:Transcription, Genetic; D014764:Viral Proteins; D001616:beta-Galactosidase / Q000235:genetics* / Q000378:metabolism
D015704:CD4 Antigens; D016827:CD8 Antigens; D015342:DNA Probes; D014764:Viral Proteins; D013006:Growth Hormone; D004247:DNA; C045073:Cre recombinase; D004254:DNA Nucleotidyltransferases; D019426:Integrases; D001616:beta-Galactosidase