Utilization of phytohaemagglutinin for in vivo karyological studies in teleost fish.
A simple technique is described for increasing the number of mitoses in circulating blood and in the kidney of teleost fish. This method employed phytohaemagglutinin-P solution, a T-like cell mitogen, which was injected (1 mg/100 g body wt) intraperitoneally, and 53 h later many blasts suitable for karyological studies, blocked in metaphase, were present both in the circulating blood and in the kidney.
Baruffaldi
A
A
Institute of Comparative Anatomy, Università di Ferrara, Italy.
Lanzara
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V
Cucchi
C
C
D000818:Animals; D002397:Catfishes; D007621:Karyotyping / Q000379:methods*; D008938:Mitosis / Q000187:drug effects; D010835:Phytohemagglutinins
D010835:Phytohemagglutinins