title: 9,11-Dicis-12-fluororhodopsin. Chromophore properties from 19F-NMR studies.
abstract: From a 19F-NMR study of 9,11-dicis-12-fluororhodopsin and its photobleached product, we concluded that the initially formed chromophore retained its configuration and the photoproduct corresponded to the two-bond isomerized all-trans. Upon standing, it slowly isomerized to the 9-cis isomer. The method represents a direct, non-destructive procedure for determining configuration purity of the pigment formed. Its unique fluorine opsin shift value is consistent with the expected different orientation of the fluoro-substituent in a dicis pigment.
authors.affiliation: Department of Chemistry, University of Hawaii, Honolulu 96822.
authors.forename: L U
authors.initials: LU
authors.lastname: Colmenares
authors.forename: R S
authors.initials: RS
authors.lastname: Liu
mesh_terms: D009682:Magnetic Resonance Spectroscopy; D010777:Photochemistry; D011487:Protein Conformation; D012243:Rhodopsin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000528:radiation effects*; D013053:Spectrophotometry; D013237:Stereoisomerism
chemical_list: D012243:Rhodopsin