Diarrhoeic shellfish toxins in Adriatic Sea mussels evaluated by an ELISA method.
A competitive enzyme immunoassay (ELISA) was used to determine the presence of okadaic acid (OA) and/or dinophysistoxin-1 (DTX-1) in mussels. The accuracy and sensitivity of the ELISA method has been checked. The sensitivity of the method (100 ng/g of hepatopancreas) makes it possible to determine OA concentrations ten times lower than the tolerance limits established by the Health Authorities of many countries. For the first time, OA and/or DTX-1 were detected in mussels (Mytilus galloprovincialis) collected in different stations along the Adriatic Sea coasts.
Tubaro
A
A
Institute of Pharmacology and Pharmacognosy, University of Trieste, Italy.
Sosa
S
S
Bruno
M
M
Gucci
P M
PM
Volterra
L
L
Della Loggia
R
R
D000818:Animals; D049872:Bivalvia / Q000737:chemistry*; D003967:Diarrhea / Q000139:chemically induced*; D004797:Enzyme-Linked Immunosorbent Assay; D004988:Ethers, Cyclic / Q000032:analysis*; D005517:Foodborne Diseases / Q000209:etiology*; D008387:Marine Toxins / Q000032:analysis*; D009792:Oceans and Seas; D019319:Okadaic Acid / Q000031:analogs & derivatives; D011714:Pyrans / Q000032:analysis
D004988:Ethers, Cyclic; D008387:Marine Toxins; D011714:Pyrans; D019319:Okadaic Acid; C051904:dinophysistoxin 1