[Peripheral blood cells secreting specific antibodies after oral stimulation with a ribosomal vaccine].
This investigation was designed to investigate mechanisms underlying oral immunization in humans after ingestion of the ribosomal vaccine D53. Immunofluorescence and ELISA (Enzyme-Linked ImmunoSorbent Assay) spot techniques were used for peripheral blood studies. The first part of the investigation was a double-blind placebo-controlled study of 12 healthy volunteers; counts of cells containing immunoglobulins and cells producing specific antibodies were higher in the individuals given the oral ribosomal vaccine than in the placebo-treated controls. In the second part of the investigation, analysis of the kinetics of apparition of the immunoglobulin-containing and specific antibody-producing cells suggested prompt stimulation of Peyer patch B lymphocytes following ingestion of the vaccine. Lastly, a study of 5 children given the vaccine on a long-term basis demonstrated increased counts of both above-described cell types after one month treatment.
Zanin
C
C
Laboratoire d'Immunologie, Faculté de Médecine, Vandoeuvre-les-Nancy, France.
Hauer
S
S
Mole
C
C
Bene
M C
MC
Faure
G C
GC
D000284:Administration, Oral; D000328:Adult; D001428:Bacterial Vaccines / Q000008:administration & dosage / Q000276:immunology* / Q000627:therapeutic use; D001773:Blood Cells / Q000502:physiology*; D002648:Child; D004275:DNA, Ribosomal / Q000276:immunology*; D005260:Female; D006192:Haemophilus Infections / Q000517:prevention & control*; D006193:Haemophilus influenzae / Q000235:genetics / Q000276:immunology; D006801:Humans; D007070:Immunoglobulin A / Q000276:immunology; D007075:Immunoglobulin M / Q000276:immunology; D007710:Klebsiella Infections / Q000517:prevention & control; D007711:Klebsiella pneumoniae / Q000235:genetics / Q000276:immunology; D008297:Male; D013290:Streptococcal Infections / Q000517:prevention & control*; D013291:Streptococcus / Q000235:genetics / Q000276:immunology
D001428:Bacterial Vaccines; D004275:DNA, Ribosomal; D007070:Immunoglobulin A; D007075:Immunoglobulin M
Cellules périphériques sécrétrices d'anticorps spécifiques après stimulation orale par un vaccin ribosomal.