Liver regeneration in partially hepatectomized rats infused with carnitine and lipids.
Male Wistar rats were subjected to 65-70% hepatectomy. Immediately after surgery a 6-hour infusion was given containing 115 kJ/kg b.w. of long-chain fatty acids (Nutralipid Spofa) (N) or N combined with L-carnitine (Carnitene Sigma Tau) in doses 8 mg (12.4 mumol), 40 mg (62 mumol) and 200 mg (310 mumol)/kg b.w. The rats were killed 6, 18, 21, 24 and 30 h after surgery. The results of our study demonstrate that infusion of lipids combined with carnitine, has a stimulating dose-dependent effect on liver regeneration in partially hepatectomized rats, excluding the low dose of carnitine--8 mg/kg. The dose of carnitine of 40 mg/kg had the highest stimulating effect on the course of the liver DNA synthesis, culminating 21 h after partial hepatectomy. Later, 24 and 30 h after hepatectomy, the mitotic activity of hepatocytes was highest in rats infused with carnitine in doses of 200 mg/kg b.w.
Bláha
V
V
Department of Physiology, Faculty of Medicine, Charles University, Hradec Králové, CSFR.
Simek
J
J
Zadák
Z
Z
D000818:Animals; D002331:Carnitine / Q000494:pharmacology*; D004247:DNA / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005227:Fatty Acids / Q000378:metabolism; D006498:Hepatectomy; D008055:Lipids / Q000494:pharmacology*; D008099:Liver / Q000166:cytology / Q000378:metabolism; D008115:Liver Regeneration / Q000187:drug effects* / Q000502:physiology; D008297:Male; D008940:Mitotic Index; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors
D005227:Fatty Acids; D008055:Lipids; D004247:DNA; D002331:Carnitine