title: Bioanalysis of the investigational anti-tumour drug 5,10-dideaza-5,6,7,8-tetrahydrofolic acid by high-performance liquid chromatography with ultraviolet detection.
abstract: A high-performance liquid chromatographic (HPLC) method with ultraviolet detection at 278 nm is presented for the determination of 5,10-dideaza-5,6,7,8-tetrahydrofolic acid in plasma. Sample pretreatment was achieved by using cation-exchange solid-phase extraction columns with methotrexate as internal standard. Chromatographic separation was based on ion-pair HPLC with 1-octanesulphonic acid as the ion-pairing compound. The detection limit was 10 ng/ml using an 500-microliters sample volume. The assay was linear from the detection limit up to 5000 ng/ml with good reproducibility. The applicability of the assay was demonstrated in a study in the rat.
authors.affiliation: Department of Clinical Chemistry, Netherlands Cancer Institute, Amsterdam.
authors.forename: O
authors.initials: O
authors.lastname: van Tellingen
authors.forename: J H
authors.initials: JH
authors.lastname: Sips
authors.forename: J H
authors.initials: JH
authors.lastname: Beijnen
authors.forename: J H
authors.initials: JH
authors.lastname: Schornagel
authors.forename: W J
authors.initials: WJ
authors.lastname: Nooyen
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D005493:Folic Acid Antagonists / Q000097:blood*; D007202:Indicators and Reagents; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013056:Spectrophotometry, Ultraviolet; D013763:Tetrahydrofolates / Q000097:blood*
chemical_list: D000970:Antineoplastic Agents; D005493:Folic Acid Antagonists; D007202:Indicators and Reagents; D013763:Tetrahydrofolates; C045894:lometrexol