Carbohydrates in mammalian tryptophanyl-tRNA synthetase.
Homogeneous preparations of bovine tryptophanyl-tRNA synthetase (EC 6.1.1.2) contain monosaccharides (mannose, fucose, galactose, N-acetylglucosamine) as revealed by liquid chromatography. Their content comprises 2.5-3.0% (w/w) of the enzyme composed of two subunits (60 kDa x 2). The same set of sugars was detected in elastase and CNBr-generated fragments (with molecular masses of approx. 40 kDa and 30 kDa, respectively). It is concluded that bovine tryptophanyl-tRNA synthetase, in addition to being a metallo- and phosphoprotein, is also a glycoprotein.
Kovaleva
G K
GK
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow.
Zheltova
A O
AO
Nikitushkina
T V
TV
Egorov
T A
TA
Musoljamov
A Ch
ACh
Kisselev
L L
LL
D000818:Animals; D050260:Carbohydrate Metabolism; D002417:Cattle; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D014369:Tryptophan-tRNA Ligase / Q000302:isolation & purification / Q000378:metabolism*
D014369:Tryptophan-tRNA Ligase