A simple method using PCR for direct sequencing of genomic DNA from frozen tumor tissue embedded in optimal cutting temperature compound.
Described here is a three-day protocol that directly yields DNA sequence after isolating and PCR amplifying genomic DNA from a small sample of frozen nasopharyngeal carcinoma tissue embedded in optimal cutting temperature (OCT) compound. The method is consistently successful, reproducible and will facilitate the rapid analysis of DNA sequence from very small samples.
Sun
Y
Y
BCDP, Program Resources Inc./DynCorp, National Cancer Institute, Frederick Cancer Research and Development Center, MD 21702.
Hegamyer
G
G
Colburn
N H
NH
D001483:Base Sequence; D004273:DNA, Neoplasm / Q000235:genetics*; D005615:Freezing; D016678:Genome; D006801:Humans; D008969:Molecular Sequence Data; D009303:Nasopharyngeal Neoplasms / Q000235:genetics*; D009838:Oligodeoxyribonucleotides; D016133:Polymerase Chain Reaction / Q000379:methods*; D013696:Temperature; D016610:Tissue Embedding
D004273:DNA, Neoplasm; D009838:Oligodeoxyribonucleotides