Plasma and urine glycolate assays for differentiating the hyperoxaluria syndromes.
To differentiate hyperoxaluria syndromes we measured plasma and urine glycolate by a novel high performance liquid chromatographic procedure. Mean glycolate level was 7.9 +/- 2.4 mumol./l. in plasma and 422 +/- 137 mumol./24 hours in urine from 19 control subjects. Renal clearance was about 50% the glomerular filtration rate irrespective of the underlying disease. There was close correlation between glycolate and oxalate in plasma. Plasma glycolate was normal in all but 8 patients who had primary hyperoxaluria 1. Plasma assay detected the disease more efficiently than urine assay. Pyridoxine decreased oxalate biosynthesis in 2 of the 4 patients treated with it and glycolate assay confirmed this behavior. Glycolate excretion was significantly high in 3 of 8 patients of primary hyperoxaluria 1 patients. Idiopathic stone formers had mild increases in glycolate excretion but this was not related with oxalate excretion. Glycolate levels were normal in 5 patients with enteric hyperoxaluria. We conclude that glycolate assay is essential for identifying patients with primary hyperoxaluria 1 and may represent a valuable tool for differentiating hyperoxaluria.
Marangella
M
M
Renal Stone Laboratory, Ospedale Mauriziano Umberto I, Turin, Italy.
Petrarulo
M
M
Vitale
C
C
Cosseddu
D
D
Linari
F
F
D000293:Adolescent; D000328:Adult; D002648:Child; D003937:Diagnosis, Differential; D005260:Female; D006016:Glycolates / Q000097:blood* / Q000652:urine*; D006801:Humans; D006959:Hyperoxaluria / Q000097:blood / Q000175:diagnosis* / Q000652:urine; D008297:Male; D008875:Middle Aged; D010070:Oxalates / Q000097:blood / Q000652:urine; D013577:Syndrome
D006016:Glycolates; D010070:Oxalates; C031149:glycolic acid