Detection of multiple beta-casein (CASB) alleles by amplification created restriction sites (ACRS).
Direct sequencing of polymerase chain reaction (PCR) amplified DNA has been used to detect the DNA sequences for bovine beta-casein (CASB) A3 and B variants. Based on these sequences we have designed primers which create allele-specific restriction sites in the PCR product. Restriction analysis of PCR product generated in one reaction enable us to identify the A1, A2, A3 and B alleles of CASB rapidly without the use of radioactivity.
Lien
S
S
Department of Animal Science, Agricultural University, As, Norway.
Aleström
P
P
Klungland
H
H
Rogne
S
S
D000483:Alleles; D000818:Animals; D001483:Base Sequence; D002364:Caseins / Q000235:genetics*; D002417:Cattle; D005784:Gene Amplification; D014644:Genetic Variation; D008297:Male; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009841:Oligonucleotides; D016133:Polymerase Chain Reaction; D015183:Restriction Mapping
D002364:Caseins; D009841:Oligonucleotides