Effective method for synthetic peptide immobilization that increases the sensitivity and specificity of ELISA procedures.
A procedure is described for the immobilization of synthetic peptide antigens on a plastic solid phase for performing ELISA. The use of a streptavidin-biotinylated peptide system for coating microplates with peptide antigen markedly increased both the sensitivity and the specificity compared to a standard ELISA based on synthetic peptides. The procedure was used for the detection of HIV-1-specific antibodies.
Ivanov
V S
VS
Shemyakin Institute of Bioorganic Chemistry, Academy of Sciences of Russia, Moscow.
Suvorova
Z K
ZK
Tchikin
L D
LD
Kozhich
A T
AT
Ivanov
V T
VT
D000595:Amino Acid Sequence; D004797:Enzyme-Linked Immunosorbent Assay; D015483:HIV Antibodies / Q000032:analysis; D006679:HIV Seropositivity / Q000276:immunology; D006801:Humans; D008969:Molecular Sequence Data
D015483:HIV Antibodies