Culture and decontamination methods affecting enumeration of phages infecting Bacteroides fragilis in sewage.
A new medium has been adapted for the growth of Bacteroides fragilis so that its phages can be recovered from environmental samples, and its efficiency has been assessed. Polyvinylidene difluoride membranes allow significantly higher recoveries among different membrane filters used to decontaminate the samples. In all cases, a number of phages remain in the filters and a percentage of them can be recovered by treatment with an eluant.
Tartera
C
C
Departamento de Microbiología, Facultad de Biología, Universidad de Barcelona, Spain.
Araujo
R
R
Michel
T
T
Jofre
J
J
D001435:Bacteriophages / Q000302:isolation & purification*; D001441:Bacteroides fragilis / Q000254:growth & development / Q000302:isolation & purification*; D003470:Culture Media; D005069:Evaluation Studies as Topic; D005374:Filtration / Q000295:instrumentation; D012722:Sewage; D014773:Virology / Q000379:methods
D003470:Culture Media; D012722:Sewage