Incorporation into planar lipid bilayers of ATP-regulated K+ channels from membranes of the insulin secreting beta-cell line, HIT T15.
Ion channels were incorporated into planar lipid bilayers following fusion of vesicles from the membrane of an insulin-secreting beta-cell line, HIT T15. The channel was completely blocked by 0.5 mM ATP. The channel retained the same ATP-dependence, voltage-sensitivity and single channel conductance as the ATP-regulated K+ channel that found in isolated membrane patches.
Oosawa
Y
Y
University Laboratory of Physiology, Oxford, UK.
D000255:Adenosine Triphosphate / Q000494:pharmacology*; D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000502:physiology; D006224:Cricetinae; D004553:Electric Conductivity; D007328:Insulin / Q000378:metabolism; D000078790:Insulin Secretion; D015640:Ion Channel Gating / Q000187:drug effects; D007515:Islets of Langerhans / Q000502:physiology*; D008051:Lipid Bilayers; D008564:Membrane Potentials / Q000187:drug effects; D008647:Mesocricetus; D015221:Potassium Channels / Q000187:drug effects / Q000502:physiology*; D011336:Probability
D007328:Insulin; D008051:Lipid Bilayers; D015221:Potassium Channels; D000255:Adenosine Triphosphate