Point mutation analysis of ras genes in spontaneous and chemically induced C57Bl/10J mouse liver tumours.
The high incidence and profile of ras gene mutations reported in spontaneous and chemically induced liver tumours of the B6C3F1 mouse provides a potential means of determining in vivo genotoxicity and its relevance to carcinogenicity. We analysed spontaneous and chemically induced [with 4-amino-biphenyl (ABP), 2-acetylaminofluorene (AAF) and diethylnitrosamine (DEN)] hepatocellular tumours of the C57Bl/10J mouse for H-ras, K-ras and N-ras gene mutations to see if mutational analysis of the ras genes could be useful for such a determination in this strain. Regions of DNA spanning codons 12, 13 and 61 of the ras genes were amplified from formalin fixed liver tumour sections using the polymerase chain reaction. Mutations were detected using allele specific oligonucleotide probing and confirmed by sequencing. We have found that there are few ras mutations in either spontaneous or chemically induced liver tumours in the C57Bl/10J mouse. Out of 25 spontaneous tumours two contained an A to T transversion and one contained an A to G transition in base 2 of H-ras codon 61 and two contained a G to A transition in base 2 of K-ras codon 13 (the K-ras mutations were only faintly detectable and may be present in a subpopulation of the tumour cells). In the case of the 18 ABP induced tumours one contained a C to A transversion in base 1 of H-ras codon 61, and one contained an A to T transversion in base 2 of H-ras codon 61 and one contained a G to C transversion in base 1 of K-ras codon 13. One C to A transversion in base 1 of H-ras codon 61 was detected out of eight AAF induced tumours. Of the 25 DEN induced tumours, one contained an A to G transition and one contained an A to C transversion in base 2 of H-ras codon 61. The data indicate that at least in hepatocellular tumours of the C57Bl/10J strain and using chronic dosing regimes the ras genes do not represent markers for in vivo genotoxic activity.
Lord
P G
PG
Safety of Medicines Department, ICI Pharmaceuticals, Macclesfield, Cheshire, UK.
Hardaker
K J
KJ
Loughlin
J M
JM
Marsden
A M
AM
Orton
T C
TC
D015073:2-Acetylaminofluorene; D000236:Adenoma / Q000235:genetics; D000611:Aminobiphenyl Compounds; D000818:Animals; D001483:Base Sequence; D002277:Carcinoma / Q000235:genetics; D004247:DNA / Q000187:drug effects; D004252:DNA Mutational Analysis; D004052:Diethylnitrosamine; D011905:Genes, ras / Q000235:genetics*; D008114:Liver Neoplasms, Experimental / Q000139:chemically induced / Q000235:genetics*; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D009154:Mutation
D000611:Aminobiphenyl Compounds; C006757:4-biphenylamine; D004052:Diethylnitrosamine; D004247:DNA; D015073:2-Acetylaminofluorene