Reactivation of denatured citrate synthase.
1. The imported mitochondrial enzyme citrate synthase can be partially (less than or equal to 45%) reactivated after denaturation in guanidinium chloride, if the concentration of the denaturing agent is lowered by dialysis, rather than by dilution, when essentially no reactivation is observed. 2. The presence of a reducing agent (dithiothreitol) is necessary for regain of activity. 3. Optimum regain of activity occurs at enzyme concentrations of about 10-20 micrograms/ml; at higher concentrations there is significant formation of aggregates.
Kelly
S M
SM
Department of Biological and Molecular Sciences, University of Stirling, Scotland.
Price
N C
NC
D000818:Animals; D002942:Circular Dichroism; D002950:Citrate (si)-Synthase / Q000378:metabolism*; D003956:Dialysis; D004789:Enzyme Activation; D011489:Protein Denaturation; D013552:Swine
D002950:Citrate (si)-Synthase