The origins and kinetics of bilirubin in dogs with hepatobiliary and haemolytic diseases.
In 35 dogs with spontaneous hepatobiliary liver disease the kinetics and the sources of bilirubin were quantified. The disorders were extrahepatic bile duct obstruction (n = 4), fulminant hepatitis (n = 2), (sub)acute hepatitis (n = 5), chronic active hepatitis (CAH) with cirrhosis (n = 6), hepatic lymphosarcoma (n = 5), centrizonal necrosis secondary to haemolytic anaemia (n = 6) and other (n = 2). The plasma disappearance of [3H]bilirubin was analyzed with a two-compartment model in all dogs. The ratio early labeled/late labeled bilirubin was determined by measuring the incorporation of [14C]glycine into erythrocyte haem and faecal stercobilin. By introducing this relation in the model analysis the bilirubin production rates from erythrocyte destruction (PE), ineffective erythropoiesis (PI) and hepatic haemoprotein (PL) could be quantified. Total bilirubin turnover was increased in both primary haemolytic disease and most cases of hepatobiliary disease. Erythrocyte survival was reduced in all cases but one. The bilirubin clearance was impaired to 30-50% of the normal value in most cases of hepatobiliary disease and also in primary haemolysis. In dogs with fulminant hepatitis, and cirrhosis with or without CAH, the clearance rates were reduced to values below 15% of normal. In these dogs both an impaired clearance and an increased production were important determinants of hyperbilirubinaemia. In other cases plasma bilirubin was primarily determined by increased production. These clearances and production rates were similar in haemolysis and in many cases of primary hepatobiliary disease. The hepatic haemoprotein turnover was quite variable in all subgroups, ranging from 1-74% of the total bilirubin turnover.(ABSTRACT TRUNCATED AT 250 WORDS)
Rothuizen
J
J
Department of Clinical Sciences of Companion Animals, University of Utrecht, The Netherlands.
van den Brom
W E
WE
Fevery
J
J
D000743:Anemia, Hemolytic / Q000097:blood; D000818:Animals; D001663:Bilirubin / Q000097:blood* / Q000378:metabolism; D002250:Carbon Radioisotopes; D004285:Dogs; D004912:Erythrocytes / Q000473:pathology; D005260:Female; D005998:Glycine / Q000378:metabolism; D006461:Hemolysis; D006520:Hepatitis, Animal / Q000097:blood; D006932:Hyperbilirubinemia / Q000097:blood*; D008105:Liver Cirrhosis, Biliary / Q000097:blood; D008107:Liver Diseases / Q000097:blood*; D008297:Male; D014316:Tritium
D002250:Carbon Radioisotopes; D014316:Tritium; D001663:Bilirubin; D005998:Glycine