Both internal and external regulatory elements control expression of the pea Fed-1 gene in transgenic tobacco seedlings.
In previous studies using leaves of light-grown transgenic tobacco plants, we have shown that sequences located within the transcribed region of the pea Fed-1 gene (encoding ferredoxin I) are major cis-acting determinants of light-regulated mRNA accumulation. However, we show here that these internal sequences are less important for the Fed-1 light response in etiolated tobacco seedlings than they are in green leaves and that upstream elements confer organ specificity and contribute significantly to Fed-1 light responses in etiolated material. Light effects mediated by upstream response elements are thus most pronounced during the initial induction of gene activity, whereas internal elements play a more prominent role in modulating Fed-1 expression once the gene is already active.
Gallo-Meagher
M
M
Department of Genetics, North Carolina State University, Raleigh 27695.
Sowinski
D A
DA
Elliott
R C
RC
Thompson
W F
WF
D001483:Base Sequence; D004247:DNA; D007887:Fabaceae / Q000196:embryology / Q000235:genetics* / Q000528:radiation effects; D005288:Ferredoxins / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation / Q000528:radiation effects; D008027:Light; D008969:Molecular Sequence Data; D030821:Plants, Genetically Modified; D010946:Plants, Medicinal; D010947:Plants, Toxic; D011401:Promoter Regions, Genetic; D014026:Nicotiana / Q000196:embryology / Q000235:genetics* / Q000528:radiation effects
D005288:Ferredoxins; D004247:DNA