[Study of the interaction of triethylenethiophosphamide with nucleotides by mass spectrometry with ionization by fission fragments of californium-252].
Study of interaction of the antitumor alkylating drug triethylenethiophosphoramide (thioTEPA) with nucleotides (dGMP and dCMP) suggests highly perspective employment of 252-Cf fission fragment induced desorption mass spectrometry (252-Cf PDMS) in biochemical pharmacology. Using the 252-Cf PDMS the molecular masses of the unstable, unvolatile, high-molecular substances of biological origin and the chemical adducts or complexes with drugs can be used to establish some structural-functional parameters of the above mentioned biomolecules and their derivatives in microvolumes of the incubation medium. The resulting data may be used for modelling chemotherapeutic processes of "drug-biomolecule-target" type. Using 252-Cf PDMS the complexes (dGMP (thioTEPA) n), n = 1, 2, 3 and (dCMP (thioTEPA) n), n = 1, were obtained. Some quantitative parameters and stability of these complexes were studied. Binding of dGMP with drug in the presence of dCMP was shown preferential. The data are compatible with the predictions concerning the mechanism of the antitumor property of the thioTEPA which can be manifested in the impairment structure of DNA of the malignant cells.
Sukhodub
L F
LF
Chivanov
V D
VD
Grebenik
L I
LI
Bondarenko
P V
PV
Zubarev
R A
RA
Knysh
A N
AN
D002142:Californium; D003843:Deoxycytidine Monophosphate / Q000378:metabolism; D003848:Deoxyguanine Nucleotides / Q000378:metabolism; D007477:Ions; D013058:Mass Spectrometry / Q000379:methods; D009711:Nucleotides / Q000378:metabolism*; D013852:Thiotepa / Q000378:metabolism*
D003848:Deoxyguanine Nucleotides; D007477:Ions; D009711:Nucleotides; D003843:Deoxycytidine Monophosphate; C007257:2'-deoxyguanosine 5'-phosphate; D013852:Thiotepa; D002142:Californium
Izuchenie vzaimodeĭstviia triétilentiofosfamida s nukleotidami metodom mass-spektrometrii s ionizatsieĭ oskolkami deleniia kaliforniia-252.