Studies of actinomycin D induced B23-translocation in P388D1 cells implanted in DBA/2 mice.
Nucleophosmin/B23 is a nucleolar phosphoprotein which redistributes from nucleoli to nucleoplasm (B23-translocation) when cells are exposed to certain anticancer drugs, particularly intercalators. The B23-translocation assay has been demonstrated in cell culture to correlate with drug effects and to detect drug-resistant cells. We now report the effect of actinomycin D on B23-translocation in P388D1 cells implanted in DBA/2 mice. B23-translocation was observed in cells after actinomycin D treatment in a dosage- and time-dependent manner. Translocation could be observed within 30 min after drug treatment. Complete B23-translocation with at least 1-day duration was achieved by a single injection of 0.25 mg/kg. Reduced dosages produced partial B23-translocation with shorter durations. These results indicate that B23-translocation may be useful in monitoring drug effects in animals.
Finch
R A
RA
Department of Pharmacology, Baylor College of Medicine, Houston, TX 77030.
Chan
P K
PK
D000818:Animals; D003609:Dactinomycin / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005260:Female; D005455:Fluorescent Antibody Technique; D007941:Leukemia P388 / Q000235:genetics* / Q000473:pathology; D051379:Mice; D008811:Mice, Inbred DBA; D009368:Neoplasm Transplantation; D009687:Nuclear Proteins / Q000032:analysis / Q000235:genetics*; D000090243:Nucleophosmin; D013997:Time Factors; D014178:Translocation, Genetic / Q000187:drug effects*; D014407:Tumor Cells, Cultured / Q000187:drug effects
C000715570:Npm1 protein, mouse; D009687:Nuclear Proteins; D000090243:Nucleophosmin; D003609:Dactinomycin