Neutrophil and lymphocyte response to supplementation with vitamins C and E in young calves.
Calves, beginning at 3 d of age, were bottle-fed milk replacer unsupplemented, or supplemented with 10 g of vitamin C, or supplemented with 10 g of vitamin C plus 57 IU of vitamin E/kg of dry milk replacer (10 calves per treatment). Neutrophils from wk 2 and 4 blood samples were assayed for Staphylococcus aureus phagocytosis and neutrophil-mediated, antibody-dependent cellular cytotoxicity, and lymphocytes from those samples were assayed for concanavalin A-induced, phytohemagglutinin-induced, or pokeweed mitogen-induced proliferation. Ocular and nasal discharges of calves supplemented with vitamins C or C and E were less than those of controls for wk 1 to 8. Calves receiving supplementation with vitamin C and E tended to have higher mitogen-induced lymphocyte proliferative responses at wk 2. Neutrophil-mediated phagocytosis and antibody-dependent cellular cytotoxicity were lower at wk 2 and 4 for calves supplemented with vitamin C than for controls. Neutrophil function of calves supplemented with vitamins C and E together was near, or slightly higher than, that of unsupplemented calves for wk 2 and 4, suggesting that the addition of vitamin E negated the adverse effects that vitamin C alone had on neutrophil functions.
Eicher-Pruiett
S D
SD
Department of Animal Sciences and Industry, Kansas State University, Manhattan 66506.
Morrill
J L
JL
Blecha
F
F
Higgins
J J
JJ
Anderson
N V
NV
Reddy
P G
PG
D000818:Animals; D000920:Antibody-Dependent Cell Cytotoxicity / Q000187:drug effects; D001205:Ascorbic Acid / Q000097:blood / Q000494:pharmacology*; D002417:Cattle / Q000254:growth & development / Q000276:immunology*; D007111:Immunity, Cellular / Q000187:drug effects; D008213:Lymphocyte Activation / Q000187:drug effects; D008214:Lymphocytes / Q000187:drug effects* / Q000276:immunology; D008297:Male; D009504:Neutrophils / Q000187:drug effects* / Q000276:immunology; D014810:Vitamin E / Q000097:blood / Q000494:pharmacology*
D014810:Vitamin E; D001205:Ascorbic Acid