title: Hydrogen/deuterium isotope effect in the oscillating peroxidase-oxidase reaction.
abstract: The oscillatory peroxidase-oxidase reaction has been investigated by using NADH deuterated in the nicotinamide 4-A position. A considerable kinetic hydrogen/deuterium isotope effect on the oscillatory behavior was revealed, which may provide an additional valuable tool for mechanistic studies and for discriminating between various mechanistic models of the peroxidase-oxidase reaction. Particularly, this effect manifests in different oscillation frequencies. A sequence of simple and aperiodic oscillations was found between two stable steady states.
authors.affiliation: Laboratorium für Technische Chemie, Eidgenössische Technische Hochschule (ETH), Zurich, Switzerland.
authors.forename: P
authors.initials: P
authors.lastname: Rys
authors.forename: J
authors.initials: J
authors.lastname: Wang
mesh_terms: D003903:Deuterium; D006735:Horseradish Peroxidase / Q000378:metabolism*; D006859:Hydrogen; D007553:Isotope Labeling; D007700:Kinetics; D009243:NAD / Q000378:metabolism; D010084:Oxidation-Reduction; D013053:Spectrophotometry / Q000379:methods; D013997:Time Factors
chemical_list: D009243:NAD; D006859:Hydrogen; D003903:Deuterium; D006735:Horseradish Peroxidase