Determination of the coumarin derivative cloricromene acid in rabbit plasma and platelets.
Two methods for the determination of cloricromene acid in biological samples are described. Cloricromene acid is a catabolite of cloricromene, a coumarin derivative which is active in the cardiovascular system. After oral administration of cloricromene to a rabbit, plasma and platelets were taken at different times and cloricromene acid was then isolated by solid-phase extraction with Sep-Pak C18 cartridges using acetonitrile-tetrahydrofuran-20% aqueous acetic acid (15:11:74, v/v/v) as eluent. The analyses were performed by reversed-phase high-performance liquid chromatography (RP-HPLC) combined with fluorescence detection with excitation at 310 nm and emission at 390 nm. The limit of quantification by RP-HPLC was about 50 pg. The catabolite in the plasma was identified by continuous-flow fast atom bombardment mass spectrometry (CF-FAB-MS), also used as a complementary means of RP-HPLC determination. The results obtained by RP-HPLC and CF-FAB-MS showed good agreement.
Mariot
R
R
Department of Pharmacokinetics, Fidia Research Laboratories, Abano Terme, Italy.
Zangirolami
L
L
Bellato
P
P
Chen
S
S
Pieraccini
G
G
Moneti
G
G
D000818:Animals; D001792:Blood Platelets / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D002866:Chromonar / Q000031:analogs & derivatives* / Q000097:blood; D007202:Indicators and Reagents; D008297:Male; D011817:Rabbits; D016339:Spectrometry, Mass, Fast Atom Bombardment
D007202:Indicators and Reagents; C025945:cloricromen; D002866:Chromonar