Effects of in-vivo administration of insulin-like growth factor-I on the rate of glucose utilization in the soleus muscle of the rat.
This study investigated the effects of insulin-like growth factor-I (IGF-I) administered to rats in vivo on the soleus muscle isolated from these rats. In order to study the interactions between IGF-I and insulin, the soleus muscles were incubated in the presence of various concentrations of insulin. IGF-I (190-200 micrograms) was given twice daily; the rats were killed 1 h after one injection of IGF-I (acute administration) or after treatment with IGF-I for 10 days (prolonged administration). The level of IGF-I in plasma was increased by approximately 100% after acute administration and by around 30% after 10 days of treatment with IGF-I. Acute administration of IGF-I to the rats increased the flux of glucose to hexose monophosphate and the rates of lactate formation and glycogen synthesis in the soleus muscles; however, the responsiveness of these muscles to insulin was lost: the increase in the rate of glucose utilization by IGF-I at physiological concentrations of insulin (10 or 100 mU/l) was similar to that observed at maximal concentrations of insulin (1000 mU/l). Similar results were obtained after prolonged treatment of the rats with IGF-I; however, the increase in the rate of glucose utilization was less pronounced than when IGF-I was given acutely and the muscles were still capable of responding to insulin.(ABSTRACT TRUNCATED AT 250 WORDS)
Dimitriadis
G
G
Department of Biochemistry, University of Oxford, U.K.
Parry-Billings
M
M
Dunger
D
D
Bevan
S
S
Colquhoun
A
A
Taylor
A
A
Calder
P
P
Krause
U
U
Wegener
G
G
Newsholme
E A
EA
D000818:Animals; D005635:Fructosediphosphates / Q000378:metabolism; D005947:Glucose / Q000378:metabolism*; D006614:Hindlimb; D066298:In Vitro Techniques; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology*; D008297:Male; D009132:Muscles / Q000378:metabolism*; D010427:Pentose Phosphate Pathway; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors
D005635:Fructosediphosphates; D007334:Insulin-Like Growth Factor I; C027652:fructose 2,6-diphosphate; D005947:Glucose