Interleukin-1 is a motility factor for human breast carcinoma cells in vitro: additive effect with interleukin-6.
Interleukin-1 beta (Il-1 beta) and interleukin-1 alpha (Il-1 alpha) were shown to act as motility factors for the human breast carcinoma cell lines SK-BR-3 and ZR-75-1 in vitro. Both cytokines induced transition from the stationary to the motile phenotype (spreading). Il-1 beta stimulated translocation, shape change and random migration (chemokinesis) of SK-BR-3 cells as demonstrated by time-lapse video recordings and by a modified Boyden chamber assay. Interleukin-6 (Il-6) stimulated spreading of the SK-BR-3 cells; an additive effect with Il-1 beta on spreading and fast plasma membrane movements was evidenced. In the SK-BR-3 cell line, the signal transduction of Il-1 beta and Il-6 differed, since only the effect of Il-6 on spreading was sensitive to pertussis toxin. Both Il-1 beta and Il-6 required protein synthesis to stimulate spreading, since cycloheximide inhibited the effect of the cytokines. Induction of an autocrine loop of Il-6 in the SK-BR-3 cells by Il-1 beta was unlikely, since after stimulation with Il-1 beta, no induction of Il-6 activity was measured, nor was inhibition of stimulated spreading seen in the presence of an antiserum against Il-6. Addition of Il-8 or of an antiserum against Il-8 did not affect spreading. We concluded that Il-1 and Il-6 could act as motility factors for human breast carcinoma cells, in both an independent and an additive way.(ABSTRACT TRUNCATED AT 250 WORDS)
Verhasselt
B
B
Department of Radiotherapy and Nuclear Medicine, University Hospital, Gent/Belgium.
Van Damme
J
J
van Larebeke
N
N
Put
W
W
Bracke
M
M
De Potter
C
C
Mareel
M
M
D001943:Breast Neoplasms / Q000473:pathology*; D002462:Cell Membrane / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D048429:Cell Size / Q000187:drug effects; D017077:Culture Media, Conditioned; D003513:Cycloheximide / Q000494:pharmacology; D004347:Drug Interactions; D005260:Female; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007375:Interleukin-1 / Q000494:pharmacology*; D015850:Interleukin-6 / Q000494:pharmacology*; D037342:Pertussis Toxin; D011994:Recombinant Proteins / Q000494:pharmacology; D014407:Tumor Cells, Cultured; D014741:Video Recording; D010566:Virulence Factors, Bordetella / Q000494:pharmacology
D017077:Culture Media, Conditioned; D007375:Interleukin-1; D015850:Interleukin-6; D011994:Recombinant Proteins; D010566:Virulence Factors, Bordetella; D003513:Cycloheximide; D037342:Pertussis Toxin