title: A simple and economical strategy for downstream processing of specific antibodies to human transferrin from egg yolk.
abstract: A rapid and economical procedure for extraction of antibodies from egg yolk is described. Laying hens were immunized with human transferrin and extracts of egg-yolk were purified with a procedure based on affinity chromatography. The resulting purified antibodies were evaluated in a nephelometric system for the assay of transferrin in human sera. The results agreed closely with those obtained with a commercially available anti-human transferrin serum from rabbits.
authors.affiliation: Laboratory of Medical Biochemistry, Universitaire Instelling Antwerpen (U.I.A.), Wilrijk, Belgium.
authors.forename: V
authors.initials: V
authors.lastname: Ntakarutimana
authors.forename: P
authors.initials: P
authors.lastname: Demedts
authors.forename: M
authors.initials: M
authors.lastname: van Sande
authors.forename: S
authors.initials: S
authors.lastname: Scharpé
mesh_terms: D000906:Antibodies / Q000302:isolation & purification*; D002846:Chromatography, Affinity; D004530:Egg Yolk / Q000276:immunology*; D006801:Humans; D007114:Immunization; D007136:Immunoglobulins / Q000302:isolation & purification; D009391:Nephelometry and Turbidimetry; D014168:Transferrin / Q000276:immunology*
chemical_list: D000906:Antibodies; C016512:IgY; D007136:Immunoglobulins; D014168:Transferrin