Possibilities of the tolerance development to nipradilol and of the measurement of vascular glutathione content in isolated rat aorta.
1. Nipradilol produced concentration-dependent relaxations of isolated rat aorta ring preparations under the contracture induced by norepinephrine or KCl, which were not significantly influenced by the previous incubation with nipradilol but significantly depressed by methylene blue. 2. The previous incubation with nipradilol did not influence the relaxant responses to nitroglycerin. 3. Five different methods for measurement of vascular glutathione content did not give the satisfiable results on the detected value of glutathione content in isolated rat aorta tissues. 4. Results indicate that nipradilol will not develop the tolerance to nipradilol itself and nitroglycerin.
Nakasone
J
J
Department of Pharmacology, School of Medicine, University of the Ryukyus, Okinawa, Japan.
Matsuzaki
T
T
Aniya
Y
Y
Uezu
K
K
Sakanashi
M
M
D000818:Animals; D001013:Aorta, Thoracic / Q000187:drug effects / Q000378:metabolism; D004361:Drug Tolerance; D005978:Glutathione / Q000378:metabolism*; D066298:In Vitro Techniques; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects; D009126:Muscle Relaxation / Q000187:drug effects; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000378:metabolism*; D005996:Nitroglycerin / Q000494:pharmacology; D009638:Norepinephrine / Q000494:pharmacology; D011412:Propanolamines / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains
D011412:Propanolamines; C036089:nipradilol; D005996:Nitroglycerin; D005978:Glutathione; D009638:Norepinephrine