title: [Use of the Reflotron system for the determination of creatine kinase (CK) in the blood of swine, sheep, cattle, horses and dogs].
abstract: The Reflotron-CK test was evaluated with blood samples of healthy and diseased pigs, sheep, cattle, horses and dogs in relation to the standard CK-NAC test (UV-method). The precision within the series on the day of blood sampling was better than VK = 7.5% (coefficient of variation) with both methods. The day to day precision was estimated by using deep frozen plasma and was in the same order of magnitude with the Reflotron-CK and the UV-method (CV less than 10%). While fresh whole blood of sheep, cattle, horses and dogs respectively should be applied directly onto the dry reagent carrier with the Reflotron-pipette (32 microliters), the blood samples of pigs must be diluted (200 microliters 0.9% NaCl-solution and 32 microliters blood, factor 7.25) before determination with Reflotron-CK. In the five species mentioned the correlation between Reflotron-CK and CK values obtained with the standard UV-method was satisfactory (r greater than 0.96). Systemic deviations of the Reflotron-CK values should be corrected by calculation formulae, given in the paper.
authors.affiliation: Klinik für kleine Klauentiere und forensische Medizin, Tierärztlichen Hochschule Hannover.
authors.forename: K
authors.initials: K
authors.lastname: Bickhardt
authors.forename: C A
authors.initials: CA
authors.lastname: Carstensen
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D000829:Animals, Domestic / Q000097:blood*; D001793:Blood Preservation; D002417:Cattle / Q000097:blood; D003402:Creatine Kinase / Q000097:blood*; D004285:Dogs / Q000097:blood; D005069:Evaluation Studies as Topic; D006400:Hematocrit / Q000662:veterinary; D006736:Horses / Q000097:blood; D011237:Predictive Value of Tests; D012016:Reference Values; D012044:Regression Analysis; D015203:Reproducibility of Results; D012756:Sheep / Q000097:blood; D013552:Swine / Q000097:blood; D013696:Temperature; D013997:Time Factors
chemical_list: D003402:Creatine Kinase
vernacular_title: Anwendung des Reflotron-Systems für die Bestimmung der Kreatinkinase (CK) im Blut von Schwein, Schaf, Rind, Pferd und Hund.