Bivariate flow cytometry of farm animal chromosomes: a potential tool for gene mapping.
Bivariate flow karyotypes of chromosomes from sheep, cattle and pig lymphocytes and from a cattle-mouse somatic cell hybrid line were obtained using a dual laser fluorescence-activated cell sorter (FACS). Pig chromosomes were resolved into 19-20 peaks, indicating that most, if not all, pig chromosomes could be separated by this technique. Sheep chromosomes showed incomplete separation but three clear peaks, presumably representing the three large metacentric chromosomes, plus five other clusters were obtained. Cattle chromosomes showed poor separation but about four peaks could be distinguished, indicating that certain chromosomes could be sorted in this species. The use of cattle-mouse hybrids may enable other individual cattle chromosomes to be obtained. It is concluded that FACS separation will be a useful additional tool for gene mapping.
Dixon
S C
SC
AFRC Institute of Animal Physiology and Genetics Research, Babraham, Cambridge, UK.
Miller
N G
NG
Carter
N P
NP
Tucker
E M
EM
D000818:Animals; D000829:Animals, Domestic / Q000235:genetics*; D002417:Cattle; D002460:Cell Line; D002469:Cell Separation; D002478:Cells, Cultured; D002874:Chromosome Mapping / Q000379:methods*; D005240:Feasibility Studies; D005434:Flow Cytometry / Q000379:methods; D006822:Hybrid Cells / Q000502:physiology; D007621:Karyotyping / Q000379:methods; D008214:Lymphocytes / Q000166:cytology; D051379:Mice; D012756:Sheep; D013552:Swine