[The mechanism of action of low doses of gamma radiation connected with the development of human cell resistance to mutagens].
The human fibroblasts were gamma-irradiated with low doses (0.07-0.21 Gy). After a short time interval (3 h), a study was made of the postirradiation viability of cells (by the trypan blue dye exclusion method); post-N-methyl-N'-nitro-nitrosoguanidine-DNA synthesis (by 3H-thymidine incorporation immediately after the mutagen treatment) and postirradiation induction of DNA single-strand breaks (by alkaline elution of cells lysed on the membrane filters). The preirradiation of cells with low doses of gamma-rays was shown to render the cells resistance to induction of DNA breaks by the following exposure to gamma-radiation. The survival rate increased; DNA synthesis was resistant to alkylation damage in these cells, as compared to nonirradiated cells.
Sinel'shchikova
T A
TA
Zasukhina
G D
GD
D000220:Adaptation, Biological; D002470:Cell Survival / Q000528:radiation effects*; D004247:DNA / Q000096:biosynthesis; D004249:DNA Damage; D004261:DNA Replication; D004277:DNA, Single-Stranded / Q000528:radiation effects; D005347:Fibroblasts / Q000528:radiation effects*; D006801:Humans; D066298:In Vitro Techniques; D009153:Mutagens / Q000494:pharmacology*; D011829:Radiation Dosage; D013997:Time Factors
D004277:DNA, Single-Stranded; D009153:Mutagens; D004247:DNA
K mekhanizmu deĭstviia malykh doz gama-radiatsii, sviazannomu s formirovaniem ustoĭchivosti kletok cheloveka k mutagenam.