Evaluation of reduction-mediated labelling of antibodies with technetium-99m.
Monoclonal antibodies can be labelled with technetium-99m by prereduction of the antibody with 2-mercaptoethanol, then reduction of pertechnetate with an aliquot of a stannous kit, resulting in greater than 97% labelling without the need for further purification. The present work shows that equally high labelling can be obtained with a variety of weak ligands and that the optimum quantity of stannous chloride is 2-4 micrograms. Although the label was stable to challenge with excess DTPA, cysteine was able to remove a portion of the label. We have also shown that this technique works with the IgG2a isotype in addition to the previously reported IgG1 isotype. This approach is simple, convenient and reproducible, and warrants further clinical evaluation.
Zhang
Z M
ZM
Department of Nuclear Medicine, Ontario Cancer Institute/Princess Margaret Hospital, Toronto, Canada.
Ballinger
J R
JR
Sheldon
K
K
Boxen
I
I
D000911:Antibodies, Monoclonal; D002614:Chelating Agents / Q000737:chemistry; D002855:Chromatography, Thin Layer; D004492:Edetic Acid / Q000737:chemistry; D005069:Evaluation Studies as Topic; D007553:Isotope Labeling / Q000379:methods*; D010084:Oxidation-Reduction; D004369:Pentetic Acid / Q000737:chemistry; D013400:Sugar Acids / Q000737:chemistry; D013667:Technetium / Q000737:chemistry*; D014001:Tin / Q000737:chemistry; D017971:Tin Compounds
D000911:Antibodies, Monoclonal; D002614:Chelating Agents; D013400:Sugar Acids; D017971:Tin Compounds; C023599:stannous chloride; D013667:Technetium; D014001:Tin; D004369:Pentetic Acid; D004492:Edetic Acid; C007852:alpha-glucoheptonic acid