Effects of heparin and endothelial cell growth supplement on haemostatic functions of vascular endothelium.
Heparin in combination with endothelial cell growth factor (ECGF) affects physiological responses and growth of human umbilical vein endothelial cells (HUVEC). We have examined the effect of heparin, crude ECGF (endothelial cell growth supplement [ECGS]), or both on the basal and thrombin challenged output of metabolites by HUVEC. The supernatant and/or cell lysate was assayed for released prostacyclin, von Willebrand factor, tissue plasminogen activator, plasminogen activator inhibitor and thrombospondin. Heparin modified release of all these metabolites when in combination with ECGS, and in general these responses were the opposite of those generated by inflammatory mediators such as interleukin-1. It has been postulated that heparin acts by potentiating the effect of ECGF, but heparin inhibited thrombospondin release and enhanced that of von Willebrand factor in the absence of ECGS, while ECGS alone inhibited release of plasminogen activator inhibitor. Thus, under our experimental conditions it would appear that heparin and crude ECGF can affect HUVEC independently of one another.
Minter
A J
AJ
Department of Haematology, School of Pathology, University of New South Wales, Prince of Wales Hospital, Randwick, Australia.
Dawes
J
J
Chesterman
C N
CN
D015121:6-Ketoprostaglandin F1 alpha / Q000097:blood; D002478:Cells, Cultured; D016228:Endothelial Growth Factors / Q000494:pharmacology*; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects*; D006487:Hemostasis / Q000187:drug effects*; D006493:Heparin / Q000494:pharmacology*; D006801:Humans; D015849:Plasminogen Inactivators / Q000097:blood; D010980:Platelet Membrane Glycoproteins / Q000032:analysis; D019699:Thrombospondins; D010959:Tissue Plasminogen Activator / Q000097:blood; D014841:von Willebrand Factor / Q000032:analysis
D016228:Endothelial Growth Factors; D015849:Plasminogen Inactivators; D010980:Platelet Membrane Glycoproteins; D019699:Thrombospondins; D014841:von Willebrand Factor; D015121:6-Ketoprostaglandin F1 alpha; D006493:Heparin; D010959:Tissue Plasminogen Activator