title: Direct PCR of washed blood cells.
abstract: We report a simple and rapid method for direct DNA amplification of washed blood cells by PCR. Small samples (2-100 microliters) of blood were washed, the cells resuspended in a buffer and used directly for PCR after boiling. Amplification of a specific DNA sequence of the human transthyretin gene, directed by the primers, was successfully performed. The method gives comparable results to amplifications made by purified DNA from blood.
authors.affiliation: Department of Rheumatology, Institute of Clinical Medicine, University Hospital of Tromsø, Norway.
authors.forename: B Y
authors.initials: BY
authors.lastname: Nordvåg
authors.forename: G
authors.initials: G
authors.lastname: Husby
authors.forename: M
authors.initials: M
authors.lastname: Raafat el-Gewely
mesh_terms: D000686:Amyloidosis / Q000235:genetics; D001773:Blood Cells; D004247:DNA / Q000032:analysis; D004587:Electrophoresis, Agar Gel; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D004247:DNA