title: Detection and time-course analysis of phospholipid hydroperoxide in soybean seedlings after treatment with fungal elicitor, by chemiluminescence-HPLC assay.
abstract: Direct evidence for the existence of phosphatidylcholine hydroperoxide (PCOOH) and the determination of the changes in PCOOH after treatment of soybean seedlings with fungal elicitor, were examined using chemiluminescence-HPLC of hydroperoxide specific assay. PCOOH was transiently accumulated within 6 h after elicitor treatment but such an event was not observed in control plants, thus suggesting that accumulation of PCOOH was a response to fungal elicitor in the host plant. The result suggested that singlet oxygen had participated in the lipid hydroperoxidation, since non-conjugated fatty acid hydroperoxide isomers due to the singlet oxygen oxidation were detected in the phospholipid fraction. The reaction of lipid hydroperoxidation, preceding the induction of a defense compound, may be involved in the triggering of a plant defense mechanism against fungal invasion.
authors.affiliation: Mizutani Plant Ecochemicals Project, Research Development Corporation of Japan, Eniwa.
authors.forename: Y
authors.initials: Y
authors.lastname: Kondo
authors.forename: T
authors.initials: T
authors.lastname: Miyazawa
authors.forename: J
authors.initials: J
authors.lastname: Mizutani
mesh_terms: D001578:Benzopyrans / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002855:Chromatography, Thin Layer; D005656:Fungal Proteins / Q000302:isolation & purification / Q000494:pharmacology*; D007700:Kinetics; D008163:Luminescent Measurements; D010713:Phosphatidylcholines / Q000302:isolation & purification / Q000378:metabolism*; D010838:Phytophthora; D036343:Pterocarpans; D013025:Glycine max / Q000187:drug effects / Q000378:metabolism*; D013997:Time Factors
chemical_list: D001578:Benzopyrans; D005656:Fungal Proteins; D010713:Phosphatidylcholines; D036343:Pterocarpans; C057590:phosphatidylcholine hydroperoxide; C017343:glyceollin