title: Cytochemical and immunocytochemical characterization of a fibrillar network (GP2) in pancreatic juice: possible role as a sieve in the pancreatic ductal system.
abstract: The secretory product of the exocrine pancreas contains sedimentable and non-sedimentable materials. Electron microscopy of the pellet obtained after ultracentrifugation reveals two major components: microvesicles (pancreasomes) and a fibrillar network of small mesh size. Negative staining of an unfixed pellet demonstrated that these structures are not fixation artifacts. Cytochemical analysis showed that pancreasomes are reactive to osmication and uranyl acetate staining, whereas the fibrillar network was unreactive thereby indicating that the latter does not contain lipids; however, lead citrate staining reveals the network. Alcian blue, known to bind sulfate groups of mucosubstances, reacted strongly with the fibrillar network. The pellet was also characterized by immunocytochemistry with specific antibodies to amylase and glycoprotein 2 (GP2). Both antibodies were located only on the fibrillar network. Washing of the pellet with 100 mM KCl-250 mM NaBr had little effect on GP2 content, but reduced considerably alpha-amylase associated with the reticular matrix. It appeared that GP2 was the major component of the scaffolding that gives rise to the fibrillar network and that other proteins such as alpha-amylase could reversibly bind to it. When double-labeling immunocytochemistry was carried out on the unwashed pellet, labeling of the first antigen reduced the labeling of the second. Removal of amylase by washing the pellet increased the GP2 signal. These results indicate that amylase is bound on the GP2 network. Although the function of the GP2 network is still not clearly defined several possibilities could be envisaged at the level of the pancreatic duct system: 1) The network could drain off any aggregates or precipitates forming in small ducts. 2) The small mesh of the network would present a physical barrier to infecting bacteria that could enter into the duct system from the intestine, especially in conditions of low flow rates. 3) The network may exert a mechanical pressure on the membranes bordering the acinar lumen and small ducts thereby preventing their collapse in basal conditions.
authors.affiliation: Département de Biologie, Faculté des Sciences, Université de Sherbrooke, Québec/Canada.
authors.forename: G
authors.initials: G
authors.lastname: Grondin
authors.forename: P
authors.initials: P
authors.lastname: St-Jean
authors.forename: A R
authors.initials: AR
authors.lastname: Beaudoin
mesh_terms: D000818:Animals; D006651:Histocytochemistry; D007150:Immunohistochemistry; D008562:Membrane Glycoproteins / Q000032:analysis / Q000502:physiology* / Q000648:ultrastructure; D010183:Pancreatic Ducts / Q000648:ultrastructure*; D010189:Pancreatic Juice / Q000737:chemistry*; D013552:Swine; D000516:alpha-Amylases / Q000502:physiology
chemical_list: D008562:Membrane Glycoproteins; D000516:alpha-Amylases