title: Sequence and characterization of the gcpE gene of Escherichia coli.
abstract: In Escherichia coli, the gene gcpE encodes a protein of unknown function and lies immediately upstream of the hisS gene for histidyl-tRNA synthetase. The nucleic acid sequence of gcpE predicts an open reading frame for a protein of 40,681 Da. The probable transcription terminator of gcpE overlaps the hisS promoter. Haploid cells containing a disrupted gcpE could not be obtained, suggesting that the gcpE gene is essential.
authors.affiliation: Department of Microbiology, Southern Illinois University, Carbondale 62901.
authors.forename: J
authors.initials: J
authors.lastname: Baker
authors.forename: D B
authors.initials: DB
authors.lastname: Franklin
authors.forename: J
authors.initials: J
authors.lastname: Parker
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics* / Q000302:isolation & purification; D001483:Base Sequence; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000737:chemistry / Q000235:genetics*; D005798:Genes, Bacterial; D006643:Histidine-tRNA Ligase / Q000235:genetics; D008969:Molecular Sequence Data
chemical_list: D001426:Bacterial Proteins; D006643:Histidine-tRNA Ligase