Retinoic acid ambivalently regulates the expression of MyoD1 in the myogenic cells in the limb buds of the early developmental stages.
The expression of MyoD1 in myogenic cells located in the muscle prospective region of the limb bud at stage 20-22 was highly sensitive to retinoic acid. Unlike RAR-beta, the expression of MyoD1 mRNA in the muscle precursor cells was significantly increased by retinoic acid at lower concentrations (0.1-10 nM), but inhibited by it at higher concentrations (0.1-1 microM). The ambivalent modulation of MyoD1 expression suggested that MyoD1 expression is regulated by not only the retinoic acid receptor and its response element, but also by other factors. Retinoic acid may be involved in the differentiation of the myogenic cells during early development.
Momoi
T
T
Division of Development and Differentiation, National Institute of Neuroscience, NCNP, Tokyo, Japan.
Miyagawa-Tomita
S
S
Nakamura
S
S
Kimura
I
I
Momoi
M
M
D000818:Animals; D002454:Cell Differentiation / Q000235:genetics*; D002642:Chick Embryo; D005121:Extremities / Q000196:embryology*; D005786:Gene Expression Regulation / Q000187:drug effects*; D009132:Muscles / Q000166:cytology / Q000196:embryology* / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D013997:Time Factors; D014212:Tretinoin / Q000494:pharmacology*
D012333:RNA, Messenger; D014212:Tretinoin