Production of monoclonal antibodies specific to major allergens of Cryptomeria japonica pollen.
Monoclonal antibodies (MAbs) directed against Cryptomeria japonica pollen antigen (CPAg) were prepared. CPAg was precipitated with these MAbs and both MAbs CPA7 (IgG2a) and CPA9 (IgG1) recognized two major glycoproteins (m = 41 kDa and 46 kDa) of CPAg. MAb CPA7 or CPA9 was coupled to CNBr activated sepharose and affinity purification of the major allergen of CPAg from the crude extract was performed. The affinity purified CPAg bound IgE antibody present in patients with Cedar pollinosis.
Honda
K
K
Department of Otorhinolaryngology, School of Medicine, University of Occupational and Environmental Health, Fukuoka, Japan.
Shirae
H
H
Miwa
K
K
Matsushita
S
S
Makishima
K
K
Nishimura
Y
Y
Sasazuki
T
T
D000485:Allergens / Q000276:immunology*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D000918:Antibody Specificity; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007073:Immunoglobulin E / Q000378:metabolism; D011058:Pollen / Q000276:immunology*; D006255:Rhinitis, Allergic, Seasonal / Q000276:immunology; D014197:Trees
D000485:Allergens; D000911:Antibodies, Monoclonal; D007073:Immunoglobulin E