A method for hyperthermic treatment of mouse skin.
The Sencar mouse skin system is a recognized model for tumour initiation, promotion and progression. The current interest in the effect of hyperthermia on this multi-stage tumorigenesis model prompted the need for a technique to accurately heat a section of dorsal skin of a large number of mice for 30 min per heat treatment. In the technique described, experimental groups of 25 female Sencar mice were treated at 7-8 weeks of age under general methoxyflurane anaesthesia. Treatment consisted of the application of initiating and/or promoting agents with or without hyperthermia. For hyperthermic skin treatments, each group of mice was placed onto a platform in a water bath so that the dorsal skin of the mice was in contact with 44 degrees C temperature controlled water.
Gragtmans
N J
NJ
Atomic Energy of Canada Limited Research Company, Chalk River Nuclear Laboratories, Ontario.
Jevcak
J J
JJ
Mitchel
R E
RE
Morrison
D P
DP
McCann
R A
RA
Murphy
J W
JW
D000768:Anesthesia, General; D000818:Animals; D005260:Female; D006979:Hyperthermia, Induced / Q000379:methods*; D051379:Mice; D009374:Neoplasms, Experimental / Q000139:chemically induced / Q000401:mortality / Q000503:physiopathology*; D012878:Skin Neoplasms / Q000139:chemically induced / Q000401:mortality / Q000503:physiopathology*; D012881:Skin Temperature