title: The amplified long genomic sequence (ALGS) located in the centromeric regions of mouse chromosomes.
abstract: We reported previously that the haploid genome of standard strains of laboratory mice contains approximately 70 copies of an amplified long genomic sequence, designated ALGS, that includes a retroposon of the gene for elongation factor 2 (MER). The length of each repeating unit is more than 60 kb, and the sequence of the unit is highly conserved among the repeats. In the present study, Southern blot analysis of the genomes of wild rodents demonstrated that the ALGS is present in all subspecies of Mus musculus and is abundant in M. spicilegus, whereas it is absent in M. spretus as well as in Rattus and other closely related genera. This result indicates that the amplification occurred after the species differentiation with the genus Mus and at least prior to the differentiation of subspecies of M. musculus. To locate chromosomal positions of the ALGS, in situ hybridization was carried out with laboratory strains and wild mice. It appears that the ALGS is located in the centromeric regions of most chromosomes in laboratory mice, M. musculus and M. spicilegus, whereas no positive signals were observed with M. spretus, in accordance with the results from the Southern blotting analysis.
authors.affiliation: Department of Cell Genetics, National Institute of Genetics, Shizuoka-ken, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Koide
authors.forename: M
authors.initials: M
authors.lastname: Yoshino
authors.forename: M
authors.initials: M
authors.lastname: Niwa
authors.forename: M
authors.initials: M
authors.lastname: Ishiura
authors.forename: T
authors.initials: T
authors.lastname: Shiroishi
authors.forename: K
authors.initials: K
authors.lastname: Moriwaki
mesh_terms: D000818:Animals; D015139:Blotting, Southern; D002503:Centromere; D002874:Chromosome Mapping; D003360:Cosmids; D004247:DNA; D015342:DNA Probes; D005784:Gene Amplification; D006238:Haploidy; D007621:Karyotyping; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D009693:Nucleic Acid Hybridization; D051381:Rats
chemical_list: D015342:DNA Probes; D004247:DNA