title: Transcription of the chicken anemia virus (CAV) genome and synthesis of its 52-kDa protein.
abstract: This paper describes the expression of the chicken anemia virus (CAV) genome, a recently characterized single-stranded circular-DNA virus of a new type [Noteborn et al., J. Virol. 65 (1991) 3131-3139]. The major transcript from the CAV genome is an unspliced mRNA of about 2100 nucleotides (nt). Its transcription start point and poly(A)-addition site are located at nt 354 and 2317 of the CAV sequence, respectively. In vitro translation experiments provide evidence that the major CAV open reading frame encodes a 52-kDa protein by using the fifth AUG as a start codon of the unspliced CAV mRNA.
authors.affiliation: Laboratory for Molecular Carcinogenesis, Sylvius Laboratory, University of Leiden, The Netherlands.
authors.forename: M H
authors.initials: MH
authors.lastname: Noteborn
authors.forename: O
authors.initials: O
authors.lastname: Kranenburg
authors.forename: A
authors.initials: A
authors.lastname: Zantema
authors.forename: G
authors.initials: G
authors.lastname: Koch
authors.forename: G F
authors.initials: GF
authors.lastname: de Boer
authors.forename: A J
authors.initials: AJ
authors.lastname: van der Eb
mesh_terms: D015152:Blotting, Northern; D004267:DNA Viruses / Q000235:genetics*; D004279:DNA, Viral / Q000235:genetics; D005807:Genes, Overlapping / Q000235:genetics; D016679:Genome, Viral; D012333:RNA, Messenger / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics; D015183:Restriction Mapping; D014158:Transcription, Genetic / Q000235:genetics*; D014764:Viral Proteins / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D004279:DNA, Viral; D012333:RNA, Messenger; D012367:RNA, Viral; D014764:Viral Proteins