title: A vasotocin-like peptide in Aplysia kurodai ganglia: HPLC and RIA evidence for its identity with Lys-conopressin G.
abstract: The presence of a vasopressin (VP)- or vasotocin (VT)-like peptide in the central nervous system of the gastropod mollusc Aplysia has been indicated previously. In the case of Aplysia californica, HPLC and RIA evidence suggested the peptide was VT-like but not identical with the nonmammalian vertebrate peptide [Arg8]VT (AVT). In the present study, anterior ganglia extracts from the related species Aplysia kurodai were analyzed by HPLC followed by RIA. Further analysis of the major AVT-IR peak showed it to be indistinguishable, in three distinct solvent systems, from the sea snail venom peptide Lys-conopressin G, but to be different from the vertebrate peptides [Arg8]VP (AVP), [Lys8]VP (LVP), AVT, oxytocin (OT), mesotocin, isotocin, aspargtocin, glumitocin, and valitocin, from the sea snail venom peptide Arg-conopressin S, and from the peptides [Lys8]VT and [Gln8]OT. In addition, the carboxymethylated (CM) A. kurodai peptide had the same HPLC retention time as CM-Lys-conopressin G. The HPLC/RIA results suggest that (i) based on the properties of the solvent systems used, the A. kurodai peptide has two basic amino acids (like the conopressins but unlike the vertebrate peptides), and (ii) there is a high probability that the A. kurodai peptide is identical with Lys-conopressin G.
authors.affiliation: Department of Pharmacology and Therapeutics, Faculty of Medicine, University of Calgary, Alberta, Canada.
authors.forename: D
authors.initials: D
authors.lastname: McMaster
authors.forename: Y
authors.initials: Y
authors.lastname: Kobayashi
authors.forename: K
authors.initials: K
authors.lastname: Lederis
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001048:Aplysia / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D005724:Ganglia / Q000737:chemistry*; D008969:Molecular Sequence Data; D008978:Mollusk Venoms / Q000737:chemistry; D010121:Oxytocin / Q000031:analogs & derivatives* / Q000737:chemistry; D011863:Radioimmunoassay; D012689:Sequence Homology, Nucleic Acid; D014667:Vasopressins / Q000737:chemistry; D014668:Vasotocin / Q000737:chemistry
chemical_list: D008978:Mollusk Venoms; D014667:Vasopressins; C076621:conopressin G; D010121:Oxytocin; D014668:Vasotocin