Cloning and sequence of a gene encoding the L7/L12 ribosomal protein equivalent of Streptomyces antibioticus.
A 50S ribosomal(r) protein from the vegetative mycelium of Streptomyces antibioticus, that is absent or modified in the spore 50S r-subunit, was purified by HPLC. Determination of its N terminus and comparison with amino acid sequence data bases indicated a strong homology with the L7/L12 r-protein from Streptomyces griseus. Screening of a cosmid library of S. antibioticus chromosomal DNA with a 20-mer oligodeoxyribonucleotide probe, corresponding to an internal region of the N terminus, allowed the isolation of two hybridizing clones. A 0.90-kb HindIII-BamHI fragment from one of these clones was sequenced and found to contain a 387-bp open reading frame. The deduced gene product shows clear homology with L7/L12 r-protein equivalent from different bacteria.
Parra
F
F
Departamento de Biología Funcional, Universidad de Oviedo, Spain.
Blanco
G
G
Martín Alonso
J M
JM
Balbín
M
M
Méndez
C
C
Salas
J A
JA
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D008969:Molecular Sequence Data; D012269:Ribosomal Proteins / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D013303:Streptomyces antibioticus / Q000235:genetics*
D001426:Bacterial Proteins; D012269:Ribosomal Proteins; C016700:ribosomal protein L7-L12