Increase of plasma neuropeptide Y-like immunoreactivity following chronic hypoxia in the rat.
In an attempt to understand the changes of circulating neuropeptide Y (NPY) during hypoxia, the plasma level of NPY was investigated by radioimmunoassay. Exposure of rats to hypobaric hypoxia at an altitude of 18,000 ft for 4 weeks causes an increase of pulmonary pressure and an elevation of plasma NPY-like immunoreactivity (NPY-LI). However, the systemic blood pressure was not elevated by this chronic hypoxia. Also, plasma noradrenaline (NA) estimated by chromatographic analysis (HPLC-ECD) was not markedly raised. Failure of bretylium and guanethidine, sympathetic neuron blockers, in reducing the plasma NPY-LI level of these rats ruled out the participation of adrenergic nervous terminals. Adrenal medulla seems responsible for this elevation of plasma NPY-LI because this magnitude disappeared in adrenalectomized rats. These data suggest that chronic hypoxia induced an elevation of circulating NPY from the adrenal gland of rats.
Cheng
J T
JT
Department of Pharmacology, College of Medicine, National Cheng Kung University, Taywan City, Taiwan ROC.
Chen
C F
CF
Shum
A Y
AY
Wang
J Y
JY
Chen
H I
HI
D000311:Adrenal Glands / Q000378:metabolism*; D000315:Adrenalectomy; D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D001949:Bretylium Compounds / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006145:Guanethidine / Q000494:pharmacology; D000860:Hypoxia / Q000097:blood* / Q000378:metabolism; D008297:Male; D009478:Neuropeptide Y / Q000097:blood* / Q000187:drug effects; D009638:Norepinephrine / Q000097:blood; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains
D001949:Bretylium Compounds; D009478:Neuropeptide Y; C045166:bretylium; D009638:Norepinephrine; D006145:Guanethidine