title: Factors affecting the in vitro evolution of a myeloma cell line.
abstract: A continuously growing plasma cell line has been established from the bone marrow of a multiple myeloma patient. Initial growth of the cells was dependent on the presence of bone marrow stromal cells. Following initial outgrowth the cells were maintained by transfer onto non-autocthonous bone marrow stromal cultures. Following approximately one year of continuous growth, a subline was derived which could be grown independently of feeder cells. These stromal-cell-independent myeloma cells nevertheless retained dependence for a growth factor present in stromal-cell-conditioned media. The relevant factor in the conditioned media was determined to be interleukin-6 (IL-6). The cells also ultimately became independent of the conditioned media. These latter cells were shown to contain mRNA for IL-6 and eventually began to secrete IL-6. This cell line has thus progressed from complete dependence on stromal cells to IL-6-dependent growth in the absence of stromal cells to complete self sufficient growth. This in vitro progression may reflect an in vivo pattern of myeloma development.
authors.affiliation: Department of Medical Microbiology and Immunology, School of Medicine, University of California, Davis 95616.
authors.forename: R J
authors.initials: RJ
authors.lastname: Scibienski
authors.forename: T
authors.initials: T
authors.lastname: Paglieroni
authors.forename: V
authors.initials: V
authors.lastname: Caggiano
authors.forename: D
authors.initials: D
authors.lastname: Lemongello
authors.forename: P H
authors.initials: PH
authors.lastname: Gumerlock
authors.forename: M R
authors.initials: MR
authors.lastname: Mackenzie
mesh_terms: D001853:Bone Marrow / Q000378:metabolism / Q000473:pathology; D002455:Cell Division; D006801:Humans; D015850:Interleukin-6 / Q000096:biosynthesis / Q000235:genetics; D009101:Multiple Myeloma / Q000378:metabolism / Q000473:pathology*; D010950:Plasma Cells / Q000378:metabolism / Q000473:pathology; D012333:RNA, Messenger / Q000378:metabolism; D011971:Receptors, Immunologic / Q000378:metabolism; D019947:Receptors, Interleukin-6; D014407:Tumor Cells, Cultured / Q000378:metabolism / Q000473:pathology
chemical_list: D015850:Interleukin-6; D012333:RNA, Messenger; D011971:Receptors, Immunologic; D019947:Receptors, Interleukin-6