An investigation of the antibacterial effect of carrot on Listeria monocytogenes.
Carrot slices immersed in a potassium phosphate buffer (0.1 mol/l, pH 7.0) or carrot tissue macerated in the buffer had a lethal effect on Listeria monocytogenes. This antilisterial activity was suppressed by anaerobiosis, thiol compounds (1 mmol/l) and bovine serum albumin (0.05%) but was not affected by sodium ascorbate (200 mmol/l), propyl gallate (25 mmol/l), catalase (1100 U/ml), superoxide dismutase (357 U/ml), or chelating agents (10 mmol/l). Free-radical scavengers had no effect at 10 mmol or 50 mmol/l but histidine and diazabenzocyclooctane at 100 mmol/l reduced the antilisterial activity. The addition of Tween 20, 0.05% (v/v), to carrot macerates improved the recovery of the activity in the supernatant liquid after centrifugation at 10,000 g for 2 min. The addition of higher concentrations of the detergent to the macerate reduced the antilisterial activity.
Nguyen-The
C
C
AFRC Institute of Food Research, Norwich, UK.
Lund
B M
BM
D000693:Anaerobiosis; D000900:Anti-Bacterial Agents; D008089:Listeria monocytogenes / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology*; D010945:Plants, Edible
D000900:Anti-Bacterial Agents; D010936:Plant Extracts