Use of a recombinant antigen (OC 3.6 cDNA) for the serological diagnosis of onchocerciasis in exposed Nigerian children.
We evaluated a recombinant antigen (OC 3.6 cDNA) expressed in pMAL TM vector for the diagnosis of onchocerciasis in children living in an endemic focus of forest-type onchocerciasis in Nigeria. Using the Western blot with the maltose-binding fusion protein as antigen, 91 per cent of mf-positive children and 24 per cent of endemic normal children were positive. Furthermore, age was not a limitation to the assay. The practical limitations of the assay for field diagnosis of onchocerciasis is discussed.
Ogunrinade
A F
AF
Department of Veterinary Microbiology & Parasitology, University of Ibadan, Nigeria.
Chandrashekar
R
R
Weil
G J
GJ
Kale
O O
OO
D000818:Animals; D000947:Antigens, Helminth; D015153:Blotting, Western; D002648:Child; D006801:Humans; D009549:Nigeria; D009854:Onchocerca / Q000276:immunology; D009855:Onchocerciasis / Q000097:blood / Q000175:diagnosis*; D011994:Recombinant Proteins; D012680:Sensitivity and Specificity; D012698:Serologic Tests
D000947:Antigens, Helminth; D011994:Recombinant Proteins