Induction/repair of strand breakage in mature and nascent DNA of cultured Chinese hamster ovary cells exposed to hydrogen peroxide.
Treatment of cultured mammalian cells with hydrogen peroxide results in the production of extensive DNA damage. Strand breakage was produced at the level of either nascent or mature DNA and the former target appeared slightly more resistant than the latter. Although inhibitors of the enzyme poly(ADP-ribose) polymerase similarly retarded the repair of such lesions, removal of DNA strand breaks was much slower for the newly synthesized DNA as compared to mature DNA.
Cantoni
O
O
Istituto di Farmacologia e Farmacognosia, Università degli Studi di Urbino, Italy.
Fiorani
M
M
Mugnaini
M
M
Cattabeni
F
F
D000818:Animals; D016466:CHO Cells / Q000187:drug effects; D006224:Cricetinae; D003412:Cricetulus; D004247:DNA / Q000096:biosynthesis / Q000187:drug effects*; D004249:DNA Damage; D004260:DNA Repair; D004261:DNA Replication / Q000187:drug effects*; D005609:Free Radicals; D006861:Hydrogen Peroxide / Q000633:toxicity*; D011064:Poly Adenosine Diphosphate Ribose; D000067856:Poly(ADP-ribose) Polymerase Inhibitors
D005609:Free Radicals; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; D011064:Poly Adenosine Diphosphate Ribose; D004247:DNA; D006861:Hydrogen Peroxide