Toxicity and toxicokinetics of carbaryl in chickens and rats: a comparative study.
Carbaryl, a carbamate insecticide, exerts its toxic effect in animals by inhibiting the activity of neural acetylcholinesterase. Differences in sensitivity of this enzyme to inhibition were studied after intraperitoneal administration to chickens and rats. A dose of 900 mg/kg to chickens and 70 mg/kg to rats caused equivalent inhibition of brain cholinesterase activities (57% +/- 6 and 47% +/- 4, respectively) 60 min after administration, which was the time of maximal cholinergic signs. Signs of toxicity (salivation, respiratory distress, muscle tremors and weakness) were more pronounced in rats than in chickens when brain acetylcholinesterase was inhibited to the same extent in both species. Carboxylesterase activities in brain, liver, and plasma were also inhibited 60 min after administration of carbaryl to chickens and rats. Activities of enzymes associated with hepatic microsomes were unaffected. Specific activities of brain esterases, including acetylcholinesterase, carboxylesterase and neurotoxic esterase, were higher in untreated chickens than in untreated rats. Specific activities of liver esterases (carboxylesterase, A-esterase) were, however, 4- and 10-fold lower in untreated chickens than in untreated rats. Total clearance of carbaryl in the chicken, determined after intravenous administration of 5 mg/kg, was 0.26 +/- 0.02 l/kg/min. This value is 5.7 times higher than that reported for the rat, indicating that the relatively lower activities of esterases in the liver of chickens did not affect the clearance of this chemical in the avian species.
Ehrich
M
M
Virginia-Maryland Regional College of Veterinary Medicine, Blacksburg, Virginia 24061.
Correll
L
L
Strait
J
J
McCain
W
W
Wilcke
J
J
D000818:Animals; D012721:Carbaryl / Q000493:pharmacokinetics* / Q000633:toxicity; D002645:Chickens; D002800:Cholinesterase Inhibitors / Q000493:pharmacokinetics / Q000633:toxicity; D004305:Dose-Response Relationship, Drug; D004950:Esterases / Q000037:antagonists & inhibitors; D005260:Female; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013045:Species Specificity
D002800:Cholinesterase Inhibitors; D004950:Esterases; D012721:Carbaryl