Localization of actin in normal human hepatocytes using fluorescent phallotoxins and immunohistochemical amplification.
Two different methods, fluorescent phallotoxins and immunohistochemical amplification systems were used to visualize actin in normal human hepatocytes. With fluorescent phallotoxins (NBD-phallacidin or rhodamine phalloidin), F-actin was distributed along the plasma membranes and at the bile canaliculi. With immunohistochemical methods (biotin-avidin, biotin-streptavidin, silver enhancement), actin was found at the same level, however a cytoplasmic staining was observed and discussed as G-actin localization.
Benkoel
L
L
Laboratoire d'Anatomie Pathologique et de Cytochimie, Faculté de Médecine, Marseille, France.
Brisse
J
J
Capo
C
C
Benoliel
A M
AM
Bongrand
P
P
Garcia
T
T
Chamlian
A
A
D000199:Actins / Q000032:analysis*; D000911:Antibodies, Monoclonal; D005456:Fluorescent Dyes; D006801:Humans; D007150:Immunohistochemistry; D008099:Liver / Q000737:chemistry* / Q000166:cytology; D010590:Phalloidine / Q000031:analogs & derivatives*
D000199:Actins; D000911:Antibodies, Monoclonal; D005456:Fluorescent Dyes; D010590:Phalloidine