Induction of antiphospholipid autoantibodies by immunization with beta 2 glycoprotein I (apolipoprotein H).
A subset of patients with systemic lupus erythematosus has autoantibodies to acidic phospholipids. Since lipids are poor immunogens, the mechanism responsible for the induction of these antibodies is unclear. Immunization of a normal rabbit and normal mice with purified human beta 2-glycoprotein I (apolipoprotein H) resulted in the production of high levels of two non-cross-reactive antibody populations, anti-apolipoprotein H, and antiphospholipid. The antiphospholipid antibodies had binding specificities indistinguishable from autoantibodies obtained from human and murine lupus. These findings suggest a novel mechanism for the induction of antiphospholipid autoantibodies.
Gharavi
A E
AE
Division of Rheumatic Diseases, Hospital for Special Surgery-Cornell University Medical Center, New York 10021.
Sammaritano
L R
LR
Wen
J
J
Elkon
K B
KB
D000818:Animals; D001053:Apolipoproteins / Q000276:immunology*; D001323:Autoantibodies / Q000096:biosynthesis*; D002417:Cattle; D006023:Glycoproteins / Q000276:immunology*; D006801:Humans; D007114:Immunization; D051379:Mice; D010743:Phospholipids / Q000276:immunology*; D011817:Rabbits; D053482:beta 2-Glycoprotein I
D001053:Apolipoproteins; D001323:Autoantibodies; D006023:Glycoproteins; D010743:Phospholipids; D053482:beta 2-Glycoprotein I