Evidence that endogenous interleukin-1 is involved in leukocyte migration in acute experimental inflammation in rats and mice.
As a putative mediator of inflammation interleukin-1 has been implicated in the recruitment of leukocytes during the early stages of the inflammatory reaction. In the present report we have investigated the release of endogenous IL-1 in the rat zymosan pleurisy and in the mouse zymosan peritonitis. In both cases the release of the cytokine was maximal 4 hours after zymosan injection and appeared to be time-related to neutrophil migration into the inflammatory site. The effect of in vivo treatment with dexamethasone in rat pleurisy and with polyclonal anti-murine IL-1 beta antibody in mouse peritonitis was also assessed. The steroid reduced both cell migration and the release of IL-1-like activity as well as the formation of exudate and the release of eicosanoids. The anti-IL-1 beta serum inhibited selectively the number of neutrophil that migrated to the inflamed site (approximately 40%) and the IL-1 activity recovered in (approximately 70%) the exudate. In vitro incubation of the inflammatory exudate with polyclonal anti-murine IL-1 alpha or anti-murine IL-1 beta sera allowed the identification of the IL-1 species present. In the rat pleurisy IL-1 biological activity was mainly due to the alpha species, whereas IL-1 beta was the only species apparently present in the mouse peritoneal exudate.
Perretti
M
M
Sclavo Research Centre, Laboratory of Pharmacology, Siena, Italy.
Solito
E
E
Parente
L
L
D000818:Animals; D001202:Ascitic Fluid / Q000378:metabolism; D002634:Chemotaxis, Leukocyte / Q000502:physiology*; D007249:Inflammation / Q000473:pathology*; D007375:Interleukin-1 / Q000502:physiology*; D007700:Kinetics; D008297:Male; D051379:Mice; D010538:Peritonitis / Q000139:chemically induced / Q000473:pathology; D010996:Pleural Effusion / Q000378:metabolism; D010998:Pleurisy / Q000139:chemically induced / Q000473:pathology; D051381:Rats; D011919:Rats, Inbred Strains; D015054:Zymosan
D007375:Interleukin-1; D015054:Zymosan