title: Specific peptide-bond cleavage by microwave irradiation in weak acid solution.
abstract: A rapid and selective peptide-bond cleavage in weak acid, induced by microwave irradiation, has been developed. The specific cleavage sites of peptide bonds located only at the carboxyl- and amino-terminal ends of aspartyl residues along the peptide chain. A systematic study including the time course for the cleavage of various aspartyl-containing peptides, the effect of the acidity of the reaction solution on the completeness of peptide-bond cleavage, and the relationship between the power of microwave irradiation and the reaction time of cleavage are studied.
authors.affiliation: Institute of Biochemical Sciences, National Taiwan University, Taipei, Taiwan.
authors.forename: C Y
authors.initials: CY
authors.lastname: Wu
authors.forename: S T
authors.initials: ST
authors.lastname: Chen
authors.forename: S H
authors.initials: SH
authors.lastname: Chiou
authors.forename: K T
authors.initials: KT
authors.lastname: Wang
mesh_terms: D000595:Amino Acid Sequence; D004151:Dipeptides / Q000737:chemistry / Q000528:radiation effects; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D008872:Microwaves; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry* / Q000528:radiation effects; D012996:Solutions
chemical_list: D004151:Dipeptides; D010455:Peptides; D012996:Solutions