Cell surface modifications induced by calcium ion in the myxobacterium Stigmatella aurantiaca.
Calcium ion induces in the myxobacterium Stigmatella aurantiaca the ability to glide on solid surfaces and to become cohesive (D. F. Gilmore and D. White, J. Bacteriol. 161:113-117, 1985; B. J. Womack, D. F. Gilmore, and D. White, J. Bacteriol. 171:6093-6096, 1989). The addition of calcium ion to the growth medium resulted in the formation of extracellular fibrils, the appearance in the membrane fractions of a 30-kDa protein, and the accumulation in a low-speed centrifugal pellet of 10 polypeptides that cross-reacted with affinity-purified antibody to one of the polypeptides. One of the polypeptides, a 55-kDa protein, was present in the membrane fraction of control cells not incubated with calcium ion and was apparently translocated to the extracellular matrix during incubation in medium containing calcium ion. The 55-kDa protein was immunologically related to a 65-kDa protein located on the fibrils of another myxobacterium, Myxococcus xanthus.
Chang
B Y
BY
Department of Biology, Indiana University, Bloomington 47405.
White
D
D
D001426:Bacterial Proteins / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism; D001692:Biological Transport / Q000187:drug effects; D002118:Calcium / Q000494:pharmacology*; D002462:Cell Membrane / Q000378:metabolism*; D003429:Cross Reactions; D007150:Immunohistochemistry; D007553:Isotope Labeling; D009231:Myxococcales / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D016941:Myxococcus xanthus / Q000737:chemistry / Q000276:immunology; D016624:Negative Staining; D013347:Subcellular Fractions / Q000737:chemistry
D001426:Bacterial Proteins; D002118:Calcium