In vitro magnesium absorption and the role of intestinal motility.
Magnesium (Mg) is known as an inhibitor of spontaneously contracting muscular tissues. To increase extracellular Mg in vivo, high doses of Mg must be given orally. Therefore, we investigated the effect of different doses of Mg given from the mucosal side of the small intestine of rats. According to the model of Trendelenburg, a system for the perfusion of isolated small intestine was developed, which allows the simultaneous recording of absorption and muscle contractions. Increasing doses of Mg were applied serosally or intraluminally. Intramulinal Mg did not affect intestinal motility. In contrast, increasing concentrations of serosal Mg resulted in a 50% inhibition of motility at 2.9 mmol/l Mg. This indicates no influence on intestinal motility of high doses of Mg acting from the mucosal side. In further studies, the addition of citric acid or taurocholic acid did not alter Mg absorption. Serosally applied amiloride (1 mmol/l) inhibited absorption, but also resulted in complete loss of motility. Since in this model passive diffusion is the most important mechanism of Mg transport, a direct influence of amiloride on Mg absorption can be excluded. From these data, we conclude that intestinal motility influences absorption--also of ions in aqueous solution--and should therefore be taken into account in absorption studies.
Grimm
P
P
Department of Pharmacology and Toxicology of Nutrition, University of Hohenheim, Germany.
Nowitzki-Grimm
S
S
Doerr-Kremer
G
G
Classen
H G
HG
D000584:Amiloride / Q000494:pharmacology; D000818:Animals; D005260:Female; D005769:Gastrointestinal Motility; D007408:Intestinal Absorption / Q000187:drug effects; D008274:Magnesium / Q000493:pharmacokinetics*; D051381:Rats; D011919:Rats, Inbred Strains
D000584:Amiloride; D008274:Magnesium