[Synthesis of a bovine adrenodoxin precursor in vitro and its import into yeast mitochondria].
The bovine adrenal cortex adrenodoxin gene was inserted into pTZ19 under T7 promoter control. The adrenodoxin mRNA was synthesized with T7 RNA polymerase and then translated in the reticulocyte cell-free translation system. The protein product was identified as the adrenodoxin precursor with molecular weight 22000. The import of the precursor into isolated yeast mitochondria was carried out. The protein was found to be inserted into the trypsin-insensitive compartment of mitochondria via an energy dependent way. This resulted in the processing of the precursor to the 12000-mature form. Thus, the precursor of mammalian adrenodoxin can be normally imported into yeast mitochondria.
Novikova
L A
LA
Isaeva
L V
LV
Krynetskiĭ
E Iu
EIu
Luzikov
V N
VN
D000325:Adrenodoxin / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D000818:Animals; D002417:Cattle; D002474:Cell-Free System; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D005800:Genes, Fungal; D005814:Genes, Viral; D008928:Mitochondria / Q000378:metabolism*; D010957:Plasmids; D011401:Promoter Regions, Genetic; D014176:Protein Biosynthesis; D011498:Protein Precursors / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D013604:T-Phages / Q000235:genetics; D014162:Transfection; D014764:Viral Proteins
D011498:Protein Precursors; D014764:Viral Proteins; D000325:Adrenodoxin; C062210:bacteriophage T7 RNA polymerase; D012321:DNA-Directed RNA Polymerases
Sintez predshestvennika adrenodoksina byka in vitro i ego import v drozhzhevye mitokhondrii.