title: Xenopus laevis sperm proteins, previously identified as surface proteins with egg coat binding capability, are indeed histone H4, histone H3, and sperm specific protein SP2.
abstract: Recently, four Xenopus sperm proteins thought to be involved in binding to the egg envelope were identified (Lindsay and Hedrick, J. Exp. Zool., 245:286-293, '88). We have studied the three more abundant ones of apparent molecular weight of 14, 19, and 25 kd in SDS-PAGE. We have shown that these proteins are indeed nuclear basic proteins: the 14 kd is the histone H4, the 19 kd is the histone H3, and the 25 kd is the sperm-specific protein SP2.
authors.affiliation: Dipartimento di Biologia, Università di Milano, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Bernardini
authors.forename: I D
authors.initials: ID
authors.lastname: Donne
authors.forename: S
authors.initials: S
authors.lastname: Norreri
authors.forename: A
authors.initials: A
authors.lastname: Negri
authors.forename: A
authors.initials: A
authors.lastname: Milzani
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D006657:Histones / Q000032:analysis*; D008297:Male; D008565:Membrane Proteins / Q000032:analysis*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D013094:Spermatozoa / Q000737:chemistry*; D014982:Xenopus laevis
chemical_list: D006657:Histones; D008565:Membrane Proteins