Effects of prostaglandin F2 alpha and leukotriene D4 on pupil size, intraocular pressure, and blood-aqueous barrier in dogs.
In each of 5 groups of dogs, 0.05 ml of 1 of the following solutions was injected into the anterior chamber of both eyes: phosphate-buffered saline solution, 0.001 microgram of prostaglandin F2 alpha (PGF2 alpha), 0.01 microgram of PGF2 alpha, 0.1 microgram of leukotriene D4 (LTD4), and 1 microgram of LTD4. A 10% solution of sodium fluorescein was injected IV (14 mg/kg of body weight) at the same time, and pupil size, intraocular pressure, and anterior chamber fluorescence were measured for 1 hour after injections. In a dose-dependent manner, PGF2 alpha was a potent miotic. A significant effect on intraocular pressure was not detected when the groups given PGF2 alpha were compared with the control group. When compared with LTD4, PGF2 alpha significantly (P less than 0.05) increased the breakdown of the blood-aqueous barrier, as evidenced by increased fluorescein leakage into the anterior chamber. Leukotriene D4 caused a decrease in pupil size only at 5 minutes, compared with that of the control group. Intraocular pressure was greater (but not significantly) in the group given 1 microgram of LTD4.
Dziezyc
J
J
Department of Small Animal Medicine and Surgery, College of Veterinary Medicine, Texas A&M University, College Station 77843.
Millichamp
N J
NJ
Keller
C B
CB
Smith
W B
WB
D000818:Animals; D000867:Anterior Chamber / Q000378:metabolism; D002199:Capillary Permeability / Q000187:drug effects; D015237:Dinoprost / Q000494:pharmacology*; D004285:Dogs / Q000502:physiology*; D004305:Dose-Response Relationship, Drug; D019793:Fluorescein; D005452:Fluoresceins / Q000493:pharmacokinetics; D007429:Intraocular Pressure / Q000187:drug effects*; D011680:Pupil / Q000187:drug effects*; D013189:SRS-A / Q000494:pharmacology*
D005452:Fluoresceins; D013189:SRS-A; D015237:Dinoprost; D019793:Fluorescein