Simple, rapid and sensitive determination of plasma taurine by high-performance liquid chromatography using pre-column derivative formation with fluorescamine.
A simple, rapid and sensitive method for the determination of plasma taurine by high-performance liquid chromatography in the isocratic mode has been developed. The deproteinized plasma was treated with fluorescamine. These derivatives were separated on a LiChrospher 100 RP-8 column within 15 min. The detection limit for taurine was 0.2 microM. The plasma taurine contents of yellowtail fish, Seriola quinqueradiata, beef cattle, dairy cows and chickens were determined to be 125 +/- 54, 5.6 +/- 1.4, 2.2 +/- 0.7 and 20.0 +/- 9.6 micrograms/ml, respectively.
Sakai
T
T
Applied Biochemistry Division, Faculty of Agriculture, Miyazaki University, Japan.
Nagasawa
T
T
D000818:Animals; D002417:Cattle / Q000097:blood; D002645:Chickens / Q000097:blood; D002851:Chromatography, High Pressure Liquid; D005399:Fishes / Q000097:blood; D005450:Fluorescamine; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D013654:Taurine / Q000097:blood*
D007202:Indicators and Reagents; D013654:Taurine; D005450:Fluorescamine