[The detection and characteristics of substances with fibroblast growth factor properties in the cells of loach embryos].
The substances extracted and partially purified from embryos of the loach (Misgurnus fossilis L.) at the late blastula stage are shown to possess properties characteristic of the fibroblast growth factor (FGF): 1) they bind with Heparin-Sepharose; 2) induce DNA synthesis in mouse NIH-3T3 and Swiss-3T3 fibroblast cell lines and in the primary culture of human embryonic fibroblasts; 3) have an apparent molecular weight of 15-16 kDa (according to SDS-electrophoresis); and 4) show positive reaction with antibodies against bovine basic FGF.
Iakimovich
I A
IA
Stoĭka
R S
RS
Kusen'
S I
SI
D016475:3T3 Cells / Q000187:drug effects; D000818:Animals; D002478:Cells, Cultured / Q000187:drug effects; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D003531:Cypriniformes / Q000196:embryology*; D004591:Electrophoresis, Polyacrylamide Gel; D005346:Fibroblast Growth Factors / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification* / Q000494:pharmacology; D005347:Fibroblasts / Q000187:drug effects; D006801:Humans; D015151:Immunoblotting; D051379:Mice; D008970:Molecular Weight
D005346:Fibroblast Growth Factors
Vyiavlenie i kharakteristika veshchestv so svoĭstvami faktora rosta fibroblastov v kletkakh zarodysheĭ v'iuna.