A novel galactose- and arabinose-specific lectin from the sponge Pellina semitubulosa: isolation, characterization and immunobiological properties.
A new lectin from the sponge Pellina semitubulosa is derived which was extracted and purified to homogeneity. The purified lectin is probably a hexamer of polypeptide chains (each M(r) 34,000) which are covalently linked via disulfide linkages; the isoelectric point is 6.1. The lectin displays the following specificities: D-galactose (50% inhibition of hemagglutination at 0.2 mM) = L-arabinose (0.2 mM) greater than D-fucose (1.5 mM) greater than D-glucose (3.0 mM). It precipitates human erythrocytes (A1, A2, A1B, B, and O) with a titer between 2(8) and 2(11) and erythrocytes from sheep and rabbits with a titer between 2(5) and 2(10). The Pellina lectin displays a strong mitogenic effect on spleen lymphocytes from mice. Immunochemical analyses revealed that both murine T- and B-lymphocytes display a capping of the lectin receptors on their cell surfaces after lectin treatment. Murine macrophages were found to endocytose the lectin. Pellina lectin at concentrations between 0.3 and 10.0 micrograms/ml potently enhances interleukin 1 (IL-1) release from mouse peritoneal macrophages and interleukin 2 (IL-2) production in mixed murine lymphocyte cultures.
Engel
M
M
Institut für Physiologische Chemie, Universität, Mainz, Germany.
Bachmann
M
M
Schröder
H C
HC
Rinkevich
B
B
Kljajic
Z
Z
Uhlenbruck
G
G
Müller
W E
WE
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001089:Arabinose / Q000378:metabolism*; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D005690:Galactose / Q000378:metabolism*; D006386:Hemagglutination Tests; D007375:Interleukin-1 / Q000378:metabolism; D007376:Interleukin-2 / Q000378:metabolism; D037102:Lectins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D008213:Lymphocyte Activation / Q000187:drug effects; D008214:Lymphocytes / Q000737:chemistry; D008264:Macrophages / Q000737:chemistry; D011161:Porifera / Q000737:chemistry*; D013379:Substrate Specificity
D000596:Amino Acids; D007375:Interleukin-1; D007376:Interleukin-2; D037102:Lectins; C076641:Pellina semitubulosa lectin; D001089:Arabinose; D005690:Galactose