title: A pitfall in determining the globulin/albumin ratio in amaranth grains.
abstract: In amaranth grains (Amaranthus hypochondriacus L. and Amaranthus cruentus L.), there were two albumin (Alb) fractions, Alb-1 and Alb-2; Alb-1 was extracted with water or 0.5 M NaCl, and Alb-2 was extracted with water after extracting Alb-1 and globulin. The amount of Alb-2 comprised about 30% of the total albumin and globulin content. Little or no measurable protein corresponding to the amaranth Alb-2 was extracted from seeds of buckwheat (Fagopyrum esculentum Moench), quinoa (Chenopodium quinoa L.), or barley (Hordeum vulgare L. cv Sekitorisai-1). That the globulin/albumin ratio (G/A) in amaranth grains has been conflicting among investigators probably accounts for the consideration of Alb-2 during extraction. We here report the revised G/A ratio of 0.3-0.4.
authors.affiliation: Department of Food and Nutrition, Osaka City University, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Konishi
authors.forename: J
authors.initials: J
authors.lastname: Azumaya
authors.forename: K
authors.initials: K
authors.lastname: Horikawa
authors.forename: N
authors.initials: N
authors.lastname: Nakatani
mesh_terms: D000418:Albumins / Q000032:analysis*; D000548:Amaranth Dye / Q000737:chemistry*; D005591:Chemical Fractionation; D005916:Globulins / Q000032:analysis*; D010940:Plant Proteins / Q000032:analysis
chemical_list: D000418:Albumins; D005916:Globulins; D010940:Plant Proteins; D000548:Amaranth Dye