Hydrogen/deuterium isotope effect in the oscillating peroxidase-oxidase reaction.
The oscillatory peroxidase-oxidase reaction has been investigated by using NADH deuterated in the nicotinamide 4-A position. A considerable kinetic hydrogen/deuterium isotope effect on the oscillatory behavior was revealed, which may provide an additional valuable tool for mechanistic studies and for discriminating between various mechanistic models of the peroxidase-oxidase reaction. Particularly, this effect manifests in different oscillation frequencies. A sequence of simple and aperiodic oscillations was found between two stable steady states.
Rys
P
P
Laboratorium für Technische Chemie, Eidgenössische Technische Hochschule (ETH), Zurich, Switzerland.
Wang
J
J
D003903:Deuterium; D006735:Horseradish Peroxidase / Q000378:metabolism*; D006859:Hydrogen; D007553:Isotope Labeling; D007700:Kinetics; D009243:NAD / Q000378:metabolism; D010084:Oxidation-Reduction; D013053:Spectrophotometry / Q000379:methods; D013997:Time Factors
D009243:NAD; D006859:Hydrogen; D003903:Deuterium; D006735:Horseradish Peroxidase