Biosynthesis of labeled fumonisins in liquid cultures of Fusarium moniliforme.
Fumonisins were readily produced in cultures of Fusarium moniliforme using a defined liquid medium. Addition of 200 mg of d3-methyl L-methionine to 100-ml cultures of F. moniliforme gave increased overall yields and high levels of deuterium (2H) incorporation into fumonisin B1. Approximately 90% of the resulting fumonisin B1 contained 6 deuterium atoms, while 9% of the product contained 3 deuterium atoms. Deuterium was shown to be incorporated exclusively in the methyl groups of the fumonisin backbone. The addition of as little as 5 mg of labeled methionine stimulated fumonisin production, but only about 5% of the fumonisin produced contained 3 deuterium atoms.
Plattner
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Bioactive Constituents Research Unit, USDA/ARS, Peoria, IL 61604.
Shackelford
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D003470:Culture Media; D037341:Fumonisins; D005670:Fusarium / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D015394:Molecular Structure; D009183:Mycotoxins / Q000096:biosynthesis* / Q000737:chemistry; D013110:Sphingosine / Q000737:chemistry
D003470:Culture Media; D037341:Fumonisins; D009183:Mycotoxins; C056933:fumonisin B1; D013110:Sphingosine