title: Preparative labeling of proteins with [35S]methionine.
abstract: The most common technique for preparative labeling of proteins with radioisotopes for experimental purposes utilizes 125I. This isotope has certain limitations, including the emission of gamma- and X-irradiation, the release of gaseous 125I2 from solutions of Na 125I, and the potential for concentration of 125I in thyroid glands. We have discovered a means for labeling proteins rapidly and simply with [35S]methionine. The technique is applicable to a wide variety of proteins. Antibodies labeled by our technique remain functional.
authors.affiliation: Department of Biological Sciences, University of Calgary, Alberta, Canada.
authors.forename: L W
authors.initials: LW
authors.lastname: Browder
authors.forename: J
authors.initials: J
authors.lastname: Wilkes
authors.forename: D I
authors.initials: DI
authors.lastname: Rodenhiser
mesh_terms: D000818:Animals; D004355:Drug Stability; D005612:Freeze Drying; D015151:Immunoblotting; D008715:Methionine; D010449:Peptide Mapping; D011506:Proteins / Q000302:isolation & purification; D011770:Pyruvate Kinase / Q000302:isolation & purification; D011817:Rabbits; D013462:Sulfur Radioisotopes
chemical_list: D011506:Proteins; D013462:Sulfur Radioisotopes; D008715:Methionine; D011770:Pyruvate Kinase