Regulation of ciliary neurotrophic factor expression in myelin-related Schwann cells in vivo.
Adult rat sciatic nerve is known to express high levels of ciliary neurotrophic factor (CNTF) mRNA and protein. Here we examine the cellular localization of CNTF protein and mRNA in peripheral nerve and the regulation of CNTF expression by peripheral axons. In intact nerve, CNTF immunoreactivity is found predominantly in the cytoplasm of myelin-related Schwann cells. After axotomy, CNTF immunoreactivity and mRNA levels fall dramatically and do not recover unless axons regenerate. This behavior is similar to the pattern of myelin gene expression in these nerves. We conclude that the expression of CNTF in Schwann cells depends on axon-Schwann cell interactions.
Friedman
B
B
Regeneron Pharmaceuticals Inc. Tarrytown, New York 10591.
Scherer
S S
SS
Rudge
J S
JS
Helgren
M
M
Morrisey
D
D
McClain
J
J
Wang
D Y
DY
Wiegand
S J
SJ
Furth
M E
ME
Lindsay
R M
RM
D000818:Animals; D001369:Axons / Q000502:physiology; D015152:Blotting, Northern; D015153:Blotting, Western; D020934:Ciliary Neurotrophic Factor; D003593:Cytoplasm / Q000378:metabolism; D003714:Denervation; D005786:Gene Expression Regulation; D007150:Immunohistochemistry; D009186:Myelin Sheath / Q000502:physiology*; D009409:Nerve Crush; D009416:Nerve Regeneration; D009419:Nerve Tissue Proteins / Q000032:analysis / Q000235:genetics* / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D012583:Schwann Cells / Q000378:metabolism*; D012584:Sciatic Nerve / Q000378:metabolism*
D020934:Ciliary Neurotrophic Factor; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger