Detection of tobacco smoke carcinogen-DNA adducts in cultured rat buccal mucosa cells following exposure to ethanol and total cigarette smoke condensate or chewing tobacco.
Formation of carcinogen-DNA adducts in rat oral epithelial cells after treatment with cigarette smoke condensate (CSC) or chewing tobacco in the presence of ethanol was investigated using the 32P-postlabeling procedure. Concomitant treatment of the cells with ethanol increased the relative adduct level over that found in cells treated with tobacco smoke condensate only. Treatment with chewing tobacco resulted in slightly higher adduct levels than in controls. Treatment of the cells with ethanol did not significantly increase the uptake of a polycyclic aromatic hydrocarbon, benzo[j]fluoranthene, however, high tar CSC alone or in combination with ethanol significantly increased the uptake of radiolabeled benzo[j]fluoranthene, suggesting that increased uptake of the carcinogens may be one of the synergistic mechanisms of alcohol in oral carcinogenesis.
Autrup
J L
JL
Laboratory of Environmental Carcinogenesis, Fibiger Institute, Danish Cancer Society, Copenhagen.
Hansen
C
C
Autrup
H
H
D000818:Animals; D002273:Carcinogens / Q000493:pharmacokinetics / Q000633:toxicity*; D002610:Cheek; D003043:Cocarcinogenesis; D004247:DNA / Q000187:drug effects* / Q000378:metabolism; D004249:DNA Damage; D004357:Drug Synergism; D000431:Ethanol / Q000633:toxicity*; D005449:Fluorenes / Q000493:pharmacokinetics / Q000633:toxicity; D007553:Isotope Labeling; D009061:Mouth Mucosa / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D009062:Mouth Neoplasms / Q000209:etiology; D010761:Phosphorus Radioisotopes; D010947:Plants, Toxic; D051381:Rats; D012906:Smoke / Q000009:adverse effects*; D014030:Tobacco, Smokeless / Q000633:toxicity*
D002273:Carcinogens; D005449:Fluorenes; D010761:Phosphorus Radioisotopes; D012906:Smoke; C027704:benzo(j)fluoranthene; D000431:Ethanol; D004247:DNA