Detection of molecular deletions in the Chinese DMD patients using two amplified dystrophin sequences.
This Brief Communication reports the detection of molecular deletions in Chinese DMD patients using two new amplified dystrophin DNAs involving the regions of exon 49 and 50. The results show that over 50% of the DMD deletions can be rapidly detected by PCR amplification of these two dystrophin sequences.
Zeng
Y T
YT
Shanghai Institute of Medical Genetics, Shanghai Children's Hospital, China.
Chen
M J
MJ
Ren
Z R
ZR
Huang
Y
Y
Qiu
X K
XK
Huang
S Z
SZ
D001483:Base Sequence; D002681:China; D002872:Chromosome Deletion; D004247:DNA / Q000097:blood / Q000235:genetics* / Q000302:isolation & purification; D016189:Dystrophin / Q000235:genetics*; D005091:Exons; D006801:Humans; D007962:Leukocytes / Q000502:physiology; D008969:Molecular Sequence Data; D009136:Muscular Dystrophies / Q000097:blood / Q000235:genetics*; D009838:Oligodeoxyribonucleotides; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction / Q000379:methods
D016189:Dystrophin; D009838:Oligodeoxyribonucleotides; D015345:Oligonucleotide Probes; D004247:DNA