Enteropathogenicity of non-toxigenic Vibrio cholerae O1 for adult mice.
The enteropathogenic potential of 32 Vibrio cholerae O1 isolates that do not produce cholera toxin was examined in the orally inoculated, sealed adult mouse model. Live cultures (2 x 10(10) cfu/ml) of 7/16 clinical and 6/16 environmental isolates produced a positive intestinal fluid accumulation (FA) ratio that reached near maximum at approximately 5 h post-inoculation. Colony hybridization did not detect genes for cholera toxin, Escherichia coli heat-labile and heat-stable toxins, or shiga-like toxins. FA activity did not correlate precisely with cytotoxic activities on Chinese hamster ovary (28/32 positive), Vero (29/32) or HeLa (25/32) cells. Certain clinical and environmental isolates of non-toxigenic V. cholerae O1 appear to be enteropathogenic for the mouse, providing evidence that they may have pathogenic potential for humans through an as yet undefined mechanism(s).
Moyenuddin
M
M
Laboratory of Biological Sciences, Georgia State University, Atlanta 30303.
Wachsmuth
K
K
Richardson
S H
SH
Cook
W L
WL
D000818:Animals; D016466:CHO Cells; D002771:Cholera / Q000382:microbiology*; D002772:Cholera Toxin / Q000032:analysis* / Q000235:genetics; D006224:Cricetinae; D004195:Disease Models, Animal; D006367:HeLa Cells; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism* / Q000382:microbiology; D051379:Mice; D014709:Vero Cells; D014734:Vibrio cholerae / Q000472:pathogenicity*
D002772:Cholera Toxin