Deficiency in major high molecular-weight seminal proteins in men producing poorly coagulating and alkaline liquid ejaculates.
Using SDS-PAGE three major high molecular-weight coagulum proteins (57, 75 and 79 kD) were identified in unliquefied human ejaculates. Washed coagulum was prepared at pH 4.5 and demonstrated negligible proteolysis as determined by a highly sensitive fluorescamine-based method. Laser densitometric scanning of these high molecular-weight proteins in unliquefied ejaculates in relation to their coagulational failure revealed that the total content of 57, 75 and 79 kD proteins in whole semen represented approximately 44% of levels in the normally coagulated, approximately 24% in poorly coagulated (P less than 0.005) and approximately 3% in the non-coagulated (alkaline liquid, P less than 0.001) samples. The findings imply that these predominant high molecular-weight proteins play a major role in human seminal coagulum formation.
Mandal
A
A
Department of Biochemistry, Calcutta University College of Science, India.
Bhattacharyya
A K
AK
D000328:Adult; D004591:Electrophoresis, Polyacrylamide Gel; D005782:Gels; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D008297:Male; D008970:Molecular Weight; D029584:Prostatic Secretory Proteins; D011506:Proteins / Q000032:analysis* / Q000737:chemistry; D012661:Semen / Q000737:chemistry* / Q000502:physiology; D029589:Seminal Plasma Proteins
D005782:Gels; D029584:Prostatic Secretory Proteins; D011506:Proteins; D029589:Seminal Plasma Proteins; C043076:beta-microseminoprotein