[An improved tetrazolium agar medium for testing sugar fermentation in lactobacilli].
An improved tetrazolium agar medium for testing sugar fermentation in lactobacilli is described. Basal medium 86 was essentially a modified MRS broth with the omission of glucose. The standard formula was 30 micrograms/ml of 2,3,5-triphenyltetrazolium chloride, 2% sugar to be tested, and 2% agar in medium 86. Plates were incubated anaerobically for 2 days at 37C or 5 days at 30C, depending on the strain. With three strains each of group II and III lactobacilli, colorless, fermentation-positive colonies were clearly differentiated from red, fermentation-negative colonies. For three strains of group I lactobacilli, this medium was not satisfactory because they grew poorly on it unless supplemented with a sugar.
Nagasaki
H
H
Department of Microbiology, Kochi Medical School, Japan.
Ito
K
K
Matsuzaki
S
S
Tanaka
S
S
D000362:Agar; D050260:Carbohydrate Metabolism; D003470:Culture Media; D005285:Fermentation; D007778:Lactobacillus / Q000254:growth & development / Q000378:metabolism*; D013778:Tetrazolium Salts
D003470:Culture Media; D013778:Tetrazolium Salts; C009591:triphenyltetrazolium; D000362:Agar