title: Ceruloplasmin stimulates NADH oxidation of pig liver plasma membrane.
abstract: NADH oxidation by pig liver plasma membranes is stimulated by ceruloplasmin (CUP) reaching a maximal value at 50 U/ml of CUP. NADH oxidation activated by CUP is proportional to the amount of protein. Concanavalin A (Con A) which recognizes the glucidic residues of the CUP required for binding to the receptor inhibits the NADH oxidation in a dose-responsive manner. Both adriamycin and bathophenantroline disulfonate (BPS), previously reported as transplasma membrane electron transport inhibitors, also inhibit the CUP-stimulated NADH oxidation of pig liver plasma membranes. Our results show a clear interaction between CUP and the NADH oxidase of plasma membrane, which supports an oxidative role for CUP in its growth effect.
authors.affiliation: Departamento de Biología Celular, Universidad de Córdoba, Spain.
authors.forename: F J
authors.initials: FJ
authors.lastname: Alcaín
authors.forename: J M
authors.initials: JM
authors.lastname: Villalba
authors.forename: H
authors.initials: H
authors.lastname: Löw
authors.forename: F L
authors.initials: FL
authors.lastname: Crane
authors.forename: P
authors.initials: P
authors.lastname: Navas
mesh_terms: D000818:Animals; D000968:Antimycin A / Q000494:pharmacology; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D002570:Ceruloplasmin / Q000494:pharmacology*; D003208:Concanavalin A / Q000494:pharmacology; D004317:Doxorubicin / Q000494:pharmacology; D007700:Kinetics; D008099:Liver / Q000378:metabolism*; D009243:NAD / Q000378:metabolism*; D010084:Oxidation-Reduction; D010618:Phenanthrolines / Q000494:pharmacology; D013552:Swine
chemical_list: D010618:Phenanthrolines; D009243:NAD; D003208:Concanavalin A; C017049:bathophenanthroline disulfonic acid; D000968:Antimycin A; D004317:Doxorubicin; D002570:Ceruloplasmin