Expression of c-myc protooncogene in rat lens cells during development, maturation and reversal of galactose cataracts.
It is well established that normal patterns of epithelial cell proliferation and metabolism, and of fiber cell differentiation and maturation are essential for the maintenance of transparency in the ocular lens. Several factors, including exposure to high levels of sugars, have been known to result in the compromise of lens transparency. For example, initiation of lens cell damage by galactose induces lens epithelial cells to proliferate. Elevated levels of c-myc mRNA have usually been correlated with rapid cell growth and increased entry of cells into the S phase. Therefore, changes in c-myc mRNA levels may provide an early indication of the stimulation of lens epithelial cells to proliferate and differentiate, which has been postulated to be an early and important event in response to lens cell injury by galactose. By Northern blot hybridization analysis we quantitated c-myc mRNA levels in the lens capsule epithelia of rats (1) exposed to galactose, and (2) undergoing a partial recovery from the galactose-induced cell damage. At the onset of lens cell damage, we find c-myc mRNA to elevate to 6-fold by 24 hr, and by 48 hr decreases to about 3-fold the normal levels. During recovery, c-myc mRNA continues to be expressed at high levels approaching a 10-fold increase by day 12, then decreasing to levels of about 8-fold the control by day 30. The 24 h transitory elevation in c-myc mRNA in lens epithelial cells is in accord with our previous observations on the 24 h increase in MP26, gamma crystallin and aldose reductase mRNAs following a high influx of galactose. Therefore, the elevation in c-myc mRNA as well suggest that galactose appears to cause lens cells to undergo an early transitory period of gene induction following the exposure of lens cells to galactose.
Wen
Y
Y
Laboratory for Molecular Genetics, Doheny Eye Institute and School of Dentistry, University of Southern California, Los Angeles 90033.
Shu
S
S
Unakar
N J
NJ
Bekhor
I
I
D000818:Animals; D002386:Cataract / Q000139:chemically induced / Q000209:etiology*; D004848:Epithelium / Q000378:metabolism / Q000473:pathology; D005260:Female; D005690:Galactose / Q000494:pharmacology*; D005693:Galactosemias / Q000139:chemically induced / Q000150:complications; D015870:Gene Expression; D016259:Genes, myc; D007908:Lens, Crystalline / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D011919:Rats, Inbred Strains
D012333:RNA, Messenger; D005690:Galactose