A simple enhancement method for the silver-gold-intensified diaminobenzidine reaction in the light microscopic immunoperoxidase technique.
We describe a simple and sensitive method for enhancement of the silver-gold-intensified 3,3'-diaminobenzidine (DAB) reaction demonstrating peroxidase activity. After completing silver-gold intensification of the preparations immunostained by the avidin-biotin-peroxidase method with DAB as the chromogen, the preparations were immersed in a solution containing uranyl nitrate. This new method appeared to increase the sensitivity by at least one order of magnitude as compared with silver-gold intensification alone.
Goto
S
S
Department of Neurosurgery, Kumamoto University Medical School, Japan.
Nagahiro
S
S
Ushio
Y
Y
Hofer
W
W
D015100:3,3'-Diaminobenzidine / Q000737:chemistry*; D000818:Animals; D001360:Avidin; D001710:Biotin; D006046:Gold; D007124:Immunoenzyme Techniques; D010215:Papio; D016622:Silver Staining
D001360:Avidin; D015100:3,3'-Diaminobenzidine; D001710:Biotin; D006046:Gold