title: Determination of diclazuril in animal feed by liquid chromatography.
abstract: A method is described for the determination of diclazuril (Janssen Research Compound R64433; trademark Clinacox) in chicken feed at the mg kg(-1) level. Compound R062646, a structure analogous to diclazuril, was used as the interna standard. The drug was extracted from food with acidified methanol. Diclazuril was then isolated by means of solid-phase extraction with a cartridge containing a C18 phase. The eluate was evaporated and the residue redissolved in dimethylformamide. An aliquot was injected onto a reversed-phase high-performance liquid chromatographic column and the drug substance quantified at 280 nm by an ultraviolet detector. Extraction (absolute) recoveries of 85% for both internal standard and diclazuril were obtained. The method is suitable for diclazuril concentrations ranging from 0.1 to 1.5 mg kg(-1). Method validation data are presented.
authors.affiliation: Analytical Research Department, Janssen Research Foundation, Beerse, Belgium.
authors.forename: J
authors.initials: J
authors.lastname: De Kock
authors.forename: M
authors.initials: M
authors.lastname: De Smet
authors.forename: R
authors.initials: R
authors.lastname: Sneyers
mesh_terms: D000821:Animal Feed / Q000032:analysis*; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D003049:Coccidiostats / Q000032:analysis*; D009570:Nitriles / Q000032:analysis*; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet; D014227:Triazines / Q000032:analysis*
chemical_list: D003049:Coccidiostats; D009570:Nitriles; D014227:Triazines; C057884:diclazuril