title: alpha-Tocopherol concentrations in serum and tissues of sheep fed different sources of vitamin E.
abstract: Thirty-five crossbred wethers were used to determine the concentrations of alpha-tocopherol in serum and tissues after oral supplementation of six different vitamin E product forms. Five wethers were assigned to each of the following treatments: 1) control, no supplemental vitamin E (C), 2) emulsifiable DL-alpha-tocopheryl acetate-dry (Rovimix E-50% SD), 3) nonemulsifiable DL-alpha-tocopheryl acetate-dry (Rovimix E-50% Ads), 4) emulsifiable DL-alpha-tocopheryl acetate-liquid (Rovimix E-40% Dispersible Liquid Concentrate [DLC]); 5) emulsifiable DL-alpha-tocopherol-liquid (Hoffmann-La Roche, E-40% DLC alcohol), 6) micellized DL-alpha-tocopheryl acetate-liquid (Bioglan, Inc., E-20%); and 7) micellized DL-alpha-tocopherol-liquid (Bioglan, Inc., E-20%). Animals were supplemented daily with 1,000 IU of their respective vitamin E sources for 56 d. Blood samples were collected daily from d 0 to 7 and weekly until d 56. Animals were subsequently killed by exsanguination after stunning and eight different tissues were collected for alpha-tocopherol analysis. There were effects of day, treatment, and day x treatment interaction on serum alpha-tocopherol. All supplemented groups were higher in serum alpha-tocopherol concentration than were the C wethers. The emulsifiable vitamin E alcohol liquid product form (Treatment 5) yielded higher (P less than .01) serum alpha-tocopherol concentration than the emulsifiable acetate liquid product (Treatment 4). Sheep on Treatment 5 reached maximum concentration on d 1, sheep on Treatment 6 on d 2, and the sheep on the remaining Treatments by d 3. Blood sera alpha-tocopherol concentrations stabilized by d 6 in all supplemented groups.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Animal Science, University of Florida, Gainesville 32611.
authors.forename: L
authors.initials: L
authors.lastname: Ochoa
authors.forename: L R
authors.initials: LR
authors.lastname: McDowell
authors.forename: S N
authors.initials: SN
authors.lastname: Williams
authors.forename: N
authors.initials: N
authors.lastname: Wilkinson
authors.forename: J
authors.initials: J
authors.lastname: Boucher
authors.forename: E L
authors.initials: EL
authors.lastname: Lentz
mesh_terms: D000284:Administration, Oral; D000821:Animal Feed; D000818:Animals; D001682:Biological Availability; D004655:Emulsions; D005527:Food, Fortified; D008099:Liver / Q000378:metabolism; D008297:Male; D008823:Micelles; D009206:Myocardium / Q000378:metabolism; D010179:Pancreas / Q000378:metabolism; D012756:Sheep / Q000378:metabolism*; D024505:Tocopherols; D014810:Vitamin E / Q000008:administration & dosage / Q000031:analogs & derivatives / Q000097:blood / Q000493:pharmacokinetics*; D024502:alpha-Tocopherol / Q000031:analogs & derivatives*
chemical_list: D004655:Emulsions; D008823:Micelles; D014810:Vitamin E; D024502:alpha-Tocopherol; D024505:Tocopherols