Primary isolation of human T-cell leukemia-lymphoma virus types I and II: use for confirming infection in seroindeterminate blood donors.
We describe the use of an immunofluorescence assay and coculture to confirm human T-cell leukemia-lymphoma virus (HTLV) infection. Peripheral blood mononuclear cells from 32 of 32 seropositive donors were positive in the immunofluorescence assay, and 63% of their cocultures produced p24 antigen. Specific antibodies distinguished HTLV type I (HTLV-I) from HTLV-II. HTLV-I or HTLV-II was isolated from donors with indeterminate serologic test results.
Hjelle
B
B
Department of Pathology, University of New Mexico School of Medicine, Albuquerque 87131.
Mills
R
R
Goldsmith
C
C
Swenson
S G
SG
Cyrus
S
S
D000911:Antibodies, Monoclonal; D001782:Blood Donors; D004797:Enzyme-Linked Immunosorbent Assay; D005069:Evaluation Studies as Topic; D005455:Fluorescent Antibody Technique / Q000706:statistics & numerical data; D015490:HTLV-I Infections / Q000175:diagnosis; D015491:HTLV-II Infections / Q000175:diagnosis; D015368:Human T-lymphotropic virus 1 / Q000276:immunology / Q000302:isolation & purification*; D015367:Human T-lymphotropic virus 2 / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans; D012680:Sensitivity and Specificity; D014773:Virology / Q000379:methods
D000911:Antibodies, Monoclonal