Localization of lysosomal acid phosphatase mRNA in mouse tissues.
We studied the expression of lysosomal acid phosphatase (LAP) in mouse by hybridizing Northern blots and tissue sections with the mouse LAP cDNA. Three mRNA species of 2.3, 3.2 and 5.2 KB were identified, which differ in the length of their 3' untranslated region (UTR). The 3.2 KB mRNA is expressed in equal amounts in all tissues and represents the major species in most tissues, whereas the amounts of the 2.3 and 5.2 KB species differ. In situ hybridization of different tissues of adult mice showed a uniform expression of LAP, as expected for a housekeeping gene, except in testis and brain. In testis we found an increase in the LAP mRNA level in spermatocytes. By Northern blot analysis of young mouse testis, this increase could be attributed to late pachytene primary spermatocytes or secondary spermatocytes. In brain tissue the neurons were predominantly labeled, especially the Purkinje and pyramidal cells, whereas glial cells expressed only low amounts of LAP mRNA. Very high LAP expression was also found in the epithelial cells of the choroid plexus. Analysis of LAP expression during mouse embryonic development between Days 9.5 and 17.5 revealed a prominent expression relative to other tissues in the neural tube from Day 9.5 to Day 13.5.
Geier
C
C
Institut für Biochemie II, Universität Göttingen, Federal Republic of Germany.
Kreysing
J
J
Boettcher
H
H
Pohlmann
R
R
von Figura
K
K
D000135:Acid Phosphatase / Q000235:genetics*; D000818:Animals; D015152:Blotting, Northern; D004247:DNA; D004622:Embryo, Mammalian / Q000378:metabolism; D008247:Lysosomes / Q000201:enzymology*; D008297:Male; D051379:Mice; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000378:metabolism*; D013737:Testis / Q000378:metabolism
D012333:RNA, Messenger; D004247:DNA; D000135:Acid Phosphatase