Reactive oxygen-reducing and protein-refolding activities of adult T cell leukemia-derived factor/human thioredoxin.
Reducing and protein-refolding activities of adult T cell leukemia-derived factor (ADF)/human thioredoxin were studied. Recombinant ADF/human thioredoxin produced by E. coli, which has an insulin-reducing activity as efficient as that of E. coli thioredoxin, also reduced some reactive oxygen species, such as hydrogen peroxide. Furthermore, recombinant ADF/human thioredoxin was found to have protein-refolding activity for scrambled (mispaired disulfide-containing) RNase A. Cys-31 at the active site of ADF/human thioredoxin proved essential for reducing activity, and loss of Cys-31 in ADF/human thioredoxin attenuated the protein-refolding activity. These data suggest a physiological role of ADF/human thioredoxin in protecting living cells from proteotoxicity caused by reactive oxygens in vivo.
Mitsui
A
A
Basic Research Laboratories, Ajinomoto Co., Inc., Kawasaki, Japan.
Hirakawa
T
T
Yodoi
J
J
D000595:Amino Acid Sequence; D000818:Animals; D001555:Benzene Derivatives / Q000378:metabolism; D016207:Cytokines; D004926:Escherichia coli / Q000235:genetics; D016166:Free Radical Scavengers; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D007328:Insulin / Q000378:metabolism; D007700:Kinetics; D008099:Liver / Q000201:enzymology; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*; D010084:Oxidation-Reduction; D010957:Plasmids; D051381:Rats; D011994:Recombinant Proteins / Q000378:metabolism; D015183:Restriction Mapping; D012259:Ribonuclease, Pancreatic / Q000378:metabolism; D013379:Substrate Specificity; D013880:Thioredoxin-Disulfide Reductase / Q000378:metabolism; D013879:Thioredoxins / Q000235:genetics / Q000378:metabolism*
D001555:Benzene Derivatives; D016207:Cytokines; D016166:Free Radical Scavengers; D007328:Insulin; D009363:Neoplasm Proteins; D011994:Recombinant Proteins; C055533:adult T cell leukemia-derived factor; D013879:Thioredoxins; D006861:Hydrogen Peroxide; D013880:Thioredoxin-Disulfide Reductase; D012259:Ribonuclease, Pancreatic; C007164:cumene hydroperoxide