Monoclonal antibodies to Escherichia coli beta-galactosidase and their use for detection and purification of recombinant expression products.
A panel of seven mouse monoclonal antibodies (BG-01-BG-07) was prepared against beta-galactosidase derived from E. coli. The antibodies are beta-galactosidase specific, show no cross-reactivity with other E. coli proteins and can be used for identification and characterization of beta-galactosidase fusion proteins expressed in lambda expression vectors. One of the antibodies allows a simple, one-step isolation of the fusion proteins directly from the crude bacterial lysates using immunoaffinity chromatography.
Dráber
P
P
Institute of Molecular Genetics, Czechoslovak Academy of Sciences, Prague.
Slavícková
A
A
Sládecek
M
M
Viklický
V
V
D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D006825:Hybridomas / Q000276:immunology; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D001616:beta-Galactosidase / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification
D000911:Antibodies, Monoclonal; D011993:Recombinant Fusion Proteins; D001616:beta-Galactosidase