Specific erythroid differentiation of mouse erythroleukemia cells by activins and its enhancement by retinoic acids.
Activin A has been shown to induce hemoglobin production in various hematopoietic cells. Such activities of three structurally distinct activins (activin A, activin AB, and activin B) were compared using F5-5 mouse erythroleukemia cells. Activin A and AB had similarly potent inducing activities whereas that of activin B was much lower. The erythroid inducing activity of activins was suppressed by follistatin, an activin-binding protein but not by inhibin A and inhibin B. Retinoic acids (both all-trans and 13-cis) had weak erythroid differentiation activity. In addition, clear synergistic erythroid induction occurred when retinoic acid and activin A were mixed together. These results indicate that retinoic acid may modulate activin-induced erythropoiesis in vivo.
Uchiyama
H
H
Department of Biology, Yokohama City University, Japan.
Asashima
M
M
D028341:Activins; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D004357:Drug Synergism; D004912:Erythrocytes / Q000473:pathology*; D038681:Follistatin; D006023:Glycoproteins / Q000494:pharmacology; D006801:Humans; D007265:Inhibins / Q000494:pharmacology*; D004915:Leukemia, Erythroblastic, Acute / Q000473:pathology*; D051379:Mice; D009842:Oligopeptides; D010455:Peptides / Q000494:pharmacology; D014212:Tretinoin / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D038681:Follistatin; D006023:Glycoproteins; D009842:Oligopeptides; D010455:Peptides; C044147:activin B; D028341:Activins; C048617:myelopeptides; D014212:Tretinoin; D007265:Inhibins