Inexpensive manual synthesis of antithrombotic peptides.
We describe a simple, improved method of manual synthesis of biologically active, antithrombotic peptides. The prototype peptide KYGRGDS is an effective inhibitor of platelet aggregation in vivo. This Fmoc-based method can be implemented by the research lab that would otherwise not have access to this technology because of safety or financial concerns. This method facilitates the synthesis of large numbers of peptides in quantities of 25 to 50 mg. Fmoc manual synthesis produces high quality peptides in fewer working days than current automated approaches.
Primm
J G
JG
Blood Research Institute, Blood Center of Southeastern Wisconsin, Milwaukee 53233.
Kunicki
T J
TJ
D000595:Amino Acid Sequence; D003362:Cost-Benefit Analysis; D005343:Fibrinolytic Agents / Q000138:chemical synthesis* / Q000191:economics / Q000494:pharmacology; D006801:Humans; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000138:chemical synthesis* / Q000494:pharmacology; D010974:Platelet Aggregation / Q000187:drug effects; D010975:Platelet Aggregation Inhibitors / Q000138:chemical synthesis* / Q000494:pharmacology; D012449:Safety
D005343:Fibrinolytic Agents; D010446:Peptide Fragments; D010975:Platelet Aggregation Inhibitors