Synthetic peptides comprising defined sequences of CH-2 and CH-3 domains of human IgG1 induce prostaglandin E2 production from human peripheral blood mononuclear cells.
Synthetic peptides Y48 and Y75 comprising sequences at exposed sites within the CH-2 and CH-3 domains of human IgG1 at a concentration of 10(-5) M, increase PGE2 production by human peripheral blood mononuclear cell (PBMC) cultures. An increase of leukocyte migration inhibitory factor (LMIF) production in PBMC cultures--as a result of synthetic peptide treatment--was also observed. This LMIF activity, to some extent, is attributed to the PGE2 production by the cells; the inhibition of leukocyte migration being abolished by the presence of indomethacine or antibody to PGE2.
Kádár
J
J
Second Department of Medicine, Semmelweis University, Budapest, Hungary.
Kalmár
L
L
Stanworth
D R
DR
Gergely
P
P
D002464:Cell Migration Inhibition; D002478:Cells, Cultured; D015232:Dinoprostone / Q000096:biosynthesis*; D006801:Humans; D007141:Immunoglobulin Fc Fragments; D007074:Immunoglobulin G; D007961:Leukocyte Migration-Inhibitory Factors / Q000096:biosynthesis; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000378:metabolism; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000494:pharmacology*; D013268:Stimulation, Chemical
D007141:Immunoglobulin Fc Fragments; D007074:Immunoglobulin G; D007961:Leukocyte Migration-Inhibitory Factors; D010446:Peptide Fragments; D015232:Dinoprostone