title: Comparison of cysticercus extract, cyst fluid and Taenia saginata extract for use in ELISA for serodiagnosis of neurocysticercosis.
abstract: Cysticercus cellulosae extract (CS), cyst fluid (CF), and an extract of Taenia saginata adult worm (TS) were evaluated for use in an enzyme-linked immunosorbent assay (ELISA) for serodiagnosis of human neurocysticercosis in Thai patients. ELISA sensitivity was found to be 78.13%, 81.25% and 62.50%, respectively. False positivity was 6.66% with CS and 0% with other antigens. CF gave positivity with a pooled visceral gnathostomiasis serum and 3 of 10 (30%) of angiostrongyliasis sera. CS produced weakly positive ELISA with pooled opisthorchiasis and visceral gnathostomiasis sera. TS gave weak positive ELISA with a pooled opisthorchiasis serum. It was concluded that CF was the best antigen for use in ELISA for serodiagnosis of human neurocysticercosis.
authors.affiliation: Department of Parasitology, Faculty of Medicine, Chiang Mai University, Thailand.
authors.forename: N
authors.initials: N
authors.lastname: Morakote
authors.forename: W
authors.initials: W
authors.lastname: Nawacharoen
authors.forename: K
authors.initials: K
authors.lastname: Sukonthasun
authors.forename: W
authors.initials: W
authors.lastname: Thammasonthi
authors.forename: C
authors.initials: C
authors.lastname: Khamboonruang
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D000947:Antigens, Helminth / Q000097:blood; D001927:Brain Diseases / Q000097:blood / Q000175:diagnosis* / Q000453:epidemiology; D003551:Cysticercosis / Q000097:blood / Q000175:diagnosis* / Q000453:epidemiology; D003552:Cysticercus / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods* / Q000592:standards; D005069:Evaluation Studies as Topic; D005260:Female; D006785:Hospitals, University; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D008297:Male; D008875:Middle Aged; D015995:Prevalence; D012680:Sensitivity and Specificity; D013621:Taenia / Q000737:chemistry*; D013785:Thailand / Q000453:epidemiology; D014020:Tissue Extracts
chemical_list: D000947:Antigens, Helminth; D007074:Immunoglobulin G; D014020:Tissue Extracts