Follow-up of patent and subpatent parasitemias and development of muscular lesions in mice inoculated with very small numbers of Trypanosoma cruzi.
A sequential analysis of patent and subpatent parasitemias, mortality, and histopathology during acute Chagas' disease experimentally produced by inoculation of 10 or 100 bloodstream forms of Trypanosoma cruzi Y strain in susceptible mice was carried out. Parasites were searched for comparatively using three different methods: direct counting, Ficoll-MI density flotation, and hemoculture. Ficoll-MI density flotation promptly discriminated with high reproducibility subpatent parasitemic states not detected in the blood samples analyzed by direct counting. Despite the high proportion of supposedly uninfected animals and depending on the postinfection time, the majority of the mice had bloodstream parasites at the subpatent level detected by Ficoll-MI, and all of them had muscular lesions during the acute phase. All Ficoll-MI-negative blood samples from infected mice were also negative by hemoculture. Normal mouse blood purposely contaminated with parasite quantities ranging from 200 to 2000/ml was tested comparatively by density flotation and hemoculture and showed frequencies of reisolation varying from 25 to 100%. Overall, these data showed that inoculum as low as 10 infective forms of Y strain is able to induce acute Chagas' disease in susceptible mice and that a subpatent parasitemic state of 600-1000 forms/ml is a common finding. The use of Ficoll-MI to detect subpatent parasitemia is discussed.
Mortatti
R C
RC
Department of Immunology, Federal University Rio de Janeiro, Brazil.
Fonseca
L S
LS
Coelho
J
J
Oliveira
A
A
Moreno
M
M
D000208:Acute Disease; D000818:Animals; D001769:Blood / Q000469:parasitology; D014355:Chagas Disease / Q000097:blood / Q000469:parasitology* / Q000473:pathology; D005260:Female; D006321:Heart / Q000469:parasitology; D051379:Mice; D008807:Mice, Inbred BALB C; D009132:Muscles / Q000469:parasitology* / Q000473:pathology; D009206:Myocardium / Q000473:pathology; D010274:Parasitology / Q000379:methods; D014349:Trypanosoma cruzi / Q000302:isolation & purification*