In vitro and in vivo ultraviolet-induced alterations of oxy- and deoxyhemoglobin.
Ultraviolet (UV) radiation was found to convert oxyhemoglobin and deoxyhemoglobin stoichiometrically into methemoglobin and a met-like product, respectively. The peak conversion efficiency for oxyhemoglobin occurred at 285 nm and decreased by a factor of 100 by 315 nm. The peak conversion efficiency for deoxyhemoglobin occurred at 290 nm and decreased by a factor of 30 by 320 nm. The transformation of oxyhemoglobin to methemoglobin was also documented in intact erythrocytes using UV-B radiation. Finally, similar transformations were found to occur in human skin with UV-B exposure but not on all volunteers tested. These results imply that methemoglobin will be formed in vivo on solar exposure and provide evidence that UV-B photons reach the blood vessels.
Kollias
N
N
Wellman Laboratories of Photomedicine, Massachusetts General Hospital, Harvard Medical School, Boston 02114.
Baqer
A
A
Sadiq
I
I
Sayre
R M
RM
D006454:Hemoglobins / Q000737:chemistry / Q000378:metabolism / Q000528:radiation effects*; D006801:Humans; D066298:In Vitro Techniques; D008706:Methemoglobin / Q000378:metabolism; D010108:Oxyhemoglobins / Q000737:chemistry / Q000378:metabolism / Q000528:radiation effects*; D010777:Photochemistry; D012867:Skin / Q000528:radiation effects; D013053:Spectrophotometry; D014466:Ultraviolet Rays
D006454:Hemoglobins; D010108:Oxyhemoglobins; C100003:deoxyhemoglobin; D008706:Methemoglobin