A novel monoclonal antibody against carbohydrates of L1 cell adhesion molecule causes an influx of calcium in cultured cortical neurons.
We have studied the function of carbohydrates of the L1 molecule, a member of the immunoglobulin superfamily of adhesion molecules, using a novel monoclonal antibody, mAb-L1(2E12), against L1 molecule. This antibody was specific for the 200 kDa component of mouse L1 molecule and its epitope was N-linked for complex-type oligosaccharides. The mAb-L1(2E12) was found to induce a rise in intracellular Ca2+ concentration ([Ca2+]i) in cultured mouse embryonic cortical neurons. The rise in [Ca2+]i was dependent on the concentrations of mAb-L1(2E12). The rise seemed to be due to an influx of extracellular Ca2+ as EGTA treatment abolished it. Both cadmium and nifedipine blocked the effect of mAb-L1(2E12), suggesting the Ca2+ influx was through voltage-operated Ca2+ channels, particularly L-type Ca2+ channels. These results provide an important insight for understanding the mechanisms by which oligosaccharides of the L1 molecule influence various functions of neural cells.
Itoh
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Children's Hospital Research Foundation, Cincinnati, OH 45229-2899.
Kawamura
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Asou
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D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D000918:Antibody Specificity / Q000276:immunology; D000954:Antigens, Surface / Q000276:immunology*; D002118:Calcium / Q000378:metabolism*; D002241:Carbohydrates / Q000276:immunology*; D015816:Cell Adhesion Molecules, Neuronal / Q000737:chemistry*; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology / Q000196:embryology / Q000378:metabolism*; D016257:Fura-2; D039841:Leukocyte L1 Antigen Complex; D051379:Mice; D008813:Mice, Inbred ICR; D009457:Neuroglia / Q000378:metabolism; D011493:Protein Kinase C / Q000378:metabolism; D015290:Second Messenger Systems / Q000502:physiology
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D002241:Carbohydrates; D015816:Cell Adhesion Molecules, Neuronal; D039841:Leukocyte L1 Antigen Complex; D011493:Protein Kinase C; D002118:Calcium; D016257:Fura-2