Cloning and analysis of a gene (sms13) encoding sannamycin B-glycyltransferase from Streptomyces sannanensis and its distribution among actinomycetes.
A gene encoding sannamycin B-glycyltransferase (sms13) of Streptomyces sannanensis IFO 14239 was identified by cloning and complementation of S. sannanensis mutant SN13 which is blocked at the interconversion of sannamycins B and A. The cloned DNA fragment also permitted the conversion of fortimicin B to A both in S. sannanensis SN13 and Streptomyces lividans TK23. DNA sequences similar to sms13 were detected in all five producers of the fortimicin-group antibiotics, Micromonospora olivasterospora ATCC 21819 (fortimicin-producer), Micromonospora sp. strain SF-2098 ATCC 31580 (SF-2052), Dactylosporangium matsuzakiense ATCC 31570 (dactimicin), Streptomyces tenjimariensis ATCC 31603 (istamycin), and Saccharopolyspora hirsuta ATCC 20501 (sporaricin). This suggests that these genes of similar function from different genera were derived from a common ancestral gene.
Ohta
T
T
Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., Japan.
Hashimoto
E
E
Hasegawa
M
M
D000192:Actinomycetales / Q000235:genetics*; D000217:Acyltransferases / Q000235:genetics*; D019881:Aminoacyltransferases; D000617:Aminoglycosides; D000900:Anti-Bacterial Agents / Q000378:metabolism; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000032:analysis; D013302:Streptomyces / Q000201:enzymology / Q000235:genetics*; D014170:Transformation, Genetic
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; C025265:sannamycin B; D000217:Acyltransferases; D019881:Aminoacyltransferases; C076480:sannamycin B glycyltransferase