Solid-phase synthesis of the nucleopeptide fragment H-Asp-Ser[pAAAGTAAGCC]-Glu-OH from the nucleoprotein of Bacillus subtilis phage phi 29.
The naturally occurring DNA-nucleopeptide H-Asp-Ser[5'-pAAAGTAAGCC-3']-Glu-OH was prepared via a solid-phase phosphite triester approach using N-2-(tert-butyldiphenylsilyloxymethyl)benzoyl protected nucleosides. The oligonucleotide was linked via the extremely base-labile oxalyl ester anchor to the solid support.
Dreef-Tromp
C M
CM
Gorlaeus Laboratories, Leiden, The Netherlands.
van der Maarel
J C
JC
van den Elst
H
H
van der Marel
G A
GA
van Boom
J H
JH
D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000737:chemistry / Q000235:genetics; D001435:Bacteriophages / Q000737:chemistry* / Q000235:genetics; D001483:Base Sequence; D004279:DNA, Viral / Q000138:chemical synthesis*; D008969:Molecular Sequence Data; D009698:Nucleoproteins / Q000138:chemical synthesis*; D010446:Peptide Fragments / Q000138:chemical synthesis*; D014764:Viral Proteins / Q000138:chemical synthesis*
D004279:DNA, Viral; D009698:Nucleoproteins; D010446:Peptide Fragments; D014764:Viral Proteins