[Results of multicenter evaluation of reagents for determination of sodium, potassium and chloride ions using enzyme activation].
We present the results of a multicentre evaluation with Boehringer Mannheim/Hitachi instruments of new "enzymatic" methods for the determination of Na+, K+, and Cl- in serum or plasma. The between-day coefficient of variation was less than 1.4% (Na+), less than 2.6% (K+) and less than 1.7% (Cl-). The linear range of the assays were at least 80 to 200 mmol/l (Na+), 1.5 to 17 mmol/l (K+) and about 30 to at least 200 mmol/l (Cl-). The comparisons with routine flame atomic emission spectrometry and coulometry showed a satisfactory agreement of the test results. The "enzymatic" assays are insensitive to even grossly elevated levels of bilirubin and lipids (sodium, potassium, and chloride assays), NH4+ (potassium assay) and amylase (chloride assay). Interference by various drugs was not detected. Since the new methods can easily be adapted to photometric clinical chemistry instruments, they represent a valuable alternative to the use of ion-selective electrodes, flame atomic emission spectrometry and coulometry.
Ehrhardt
V
V
Boehringer Mannheim GmbH, Bundesrepublik Deutschland.
Baadenhuijsen
H
H
Brenna
S
S
Browne
M
M
Garcia-Beltrán
L
L
Hellsing
K
K
Külpmann
W R
WR
Paschen
K
K
Pascual-Mostaza
C
C
Pei
P
P
D002138:Calibration; D002712:Chlorides / Q000097:blood*; D004789:Enzyme Activation / Q000502:physiology*; D005069:Evaluation Studies as Topic; D006801:Humans; D011188:Potassium / Q000097:blood*; D011786:Quality Control; D012964:Sodium / Q000097:blood*; D013053:Spectrophotometry / Q000295:instrumentation*
D002712:Chlorides; D012964:Sodium; D011188:Potassium
Ergebnisse der multizentrischen Evaluierung von Reagenzien zur Bestimmung von Natrium-, Kalium- und Chloridionen mittels Enzymaktivierung.