Separation of proteins by sodium dodecyl sulfate-polyacrylamide gel electrophoresis in the presence of soluble, aqueous polymers: Ficoll and polyvinylpyrrolidone.
In previous studies we have demonstrated that water soluble polymers of dextran and methylcellulose, when incorporated into sodium dodecyl sulfate (SDS)-polyacrylamide gels, can be used to sharpen the protein bands. We have extended these studies to enhance the separations in the mid-molecular weight range. Enhanced separation of protein molecular weight markers between 30,000-67,000 was best achieved in SDS-gels containing 2.5% polyvinylpyrrolidone (average molecular weight 10,000).
Gersten
D M
DM
Department of Pathology, Georgetown University Medical Center, Washington, DC 20007.
Bijwaard
K E
KE
D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D005362:Ficoll; D008970:Molecular Weight; D011205:Povidone; D011506:Proteins / Q000302:isolation & purification*; D012995:Solubility; D014867:Water
D011506:Proteins; D014867:Water; D005362:Ficoll; D011205:Povidone