title: Molecular cloning and analysis of autonomous replicating sequence of Candida maltosa.
abstract: A Candida maltosa chromosomal DNA fragment which confers high frequency transformation of C. maltosa and autonomous replication of recombinant plasmids was cloned and sequenced. Analysis of the nucleotide sequence of the cloned DNA revealed a sequence homologous for C. maltosa autonomously replicating sequence (ARS) elements. Vector pRJ1 for C. maltosa was constructed, which contained a 1.3 kb ARS sequence, pICEM-19H and the ADE1 gene of C. maltosa. Southern blot analysis suggested that the copy number of pRJ1 in C. maltosa was approximately 20 per genome. The sequence analysis also revealed an open reading frame, encoding a polypeptide with high homology (70%) to the RS15 protein of Brugia pagangi. This open reading frame has an intron with canonical sites for correct splicing in Saccharomyces cerevisiae.
authors.affiliation: Institute of Applied Enzymology, Vilnius, Lithuanian Republic.
authors.forename: K
authors.initials: K
authors.lastname: Sasnauskas
authors.forename: R
authors.initials: R
authors.lastname: Jomantienè
authors.forename: E
authors.initials: E
authors.lastname: Lebedienè
authors.forename: J
authors.initials: J
authors.lastname: Lebedys
authors.forename: A
authors.initials: A
authors.lastname: Januska
authors.forename: A
authors.initials: A
authors.lastname: Janulaitis
mesh_terms: D000818:Animals; D001483:Base Sequence; D002009:Brugia / Q000235:genetics; D002175:Candida / Q000235:genetics*; D003001:Cloning, Molecular; D004261:DNA Replication; D005656:Fungal Proteins; D015801:Helminth Proteins; D007438:Introns; D008969:Molecular Sequence Data; D009154:Mutation; D010957:Plasmids / Q000235:genetics; D011506:Proteins / Q000235:genetics; D012326:RNA Splicing; D016364:Reading Frames; D015183:Restriction Mapping; D012269:Ribosomal Proteins; D012689:Sequence Homology, Nucleic Acid; D014170:Transformation, Genetic
chemical_list: C076363:40S ribosomal protein S13, Brugia pahangi; D005656:Fungal Proteins; D015801:Helminth Proteins; D011506:Proteins; D012269:Ribosomal Proteins; C076364:ARS protein, Candida maltosa