title: Fasciola hepatica: host responders and nonresponders to parasite glutathione S-transferase.
abstract: Fasciola hepatica glutathione S-transferase (FhGST) was isolated from adult worms by glutathione agarose affinity chromatography. SDS-PAGE shows three proteins of M(r) ranging from 29-27.8 kDa. Western immunoblot analyses using SDS-PAGE separated adult worm extracts and probed with a rabbit anti-FhGST antiserum reveal two bands in the same M(r) range. Mice and rabbits immunized with purified FhGST develop copious amounts of anti-FhGST antibodies. Moreover, antisera to F. hepatica adult worms and excretion-secretion products also react with FhGST. Cross-reactivity with schistosomes is evidenced in the reactivity with FhGST of anti-Schistosoma mansoni adult worm antisera and, to a lesser extent, antisera to S. mansoni-soluble egg antigens. The time of appearance of anti-FhGST antibodies in different species of animals infected with F. hepatica was determined. Sheep and a New Zealand white rabbit developed anti-FhGST antibodies detectable by ELISA as early as 2 weeks postexposure with F. hepatica. However, neither mice nor calves infected with F. hepatica developed antibodies to FhGST through the 5-10 weeks of infection tested. But mice infected with S. mansoni developed anti-FhGST cross-reacting antibodies by 6 weeks of infection. Calves immunized with a Fasciola/Schistosoma cross-reactive, cross-protective antigen complex in which a 12,000-kDa protein (Fh12) has been shown to contain immunoprophylactic activity, also developed antibodies to FhGST. Since FhGST is a novel potential vaccine, its protection-inducing capability in a multivalent vaccine combined with Fh12 clearly warrants study. In summary, it appears that hosts with fascioliasis are either responders to FhGST (rabbits, sheep) or nonresponders (mice, cattle), offering interesting models for studying the immune response.
authors.affiliation: Department of Pathology, University of Puerto Rico, School of Medicine, San Juan 00936-5067.
authors.forename: G V
authors.initials: GV
authors.lastname: Hillyer
authors.forename: M
authors.initials: M
authors.lastname: Soler de Galanes
authors.forename: G
authors.initials: G
authors.lastname: Battisti
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000096:biosynthesis* / Q000276:immunology; D000947:Antigens, Helminth / Q000276:immunology; D002417:Cattle; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D005210:Fasciola hepatica / Q000201:enzymology / Q000276:immunology*; D005211:Fascioliasis / Q000276:immunology*; D005982:Glutathione Transferase / Q000737:chemistry / Q000276:immunology*; D007114:Immunization; D051379:Mice; D008807:Mice, Inbred BALB C; D011817:Rabbits; D012550:Schistosoma mansoni / Q000276:immunology; D012756:Sheep; D013997:Time Factors
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D005982:Glutathione Transferase