Synthesis in vitro of very long chain fatty acids in Vibrio sp. strain ABE-1.
The activity of fatty acid synthetase (FAS) from Vibrio sp. strain ABE-1 required the presence of acyl carrier protein and was completely inhibited by thiolactomycin, an inhibitor specific for a type II FAS. These observations indicate that this enzyme is a type II FAS. Analysis by gas-liquid chromatography of the reaction products synthesized in vitro from [2-14C]malonyl-CoA by the partially purified FAS revealed, in addition to 16- and 18-carbon fatty acids which are normal constituents of this bacterium, the presence of fatty acids with very long chains. These fatty acids were identified as saturated and mono-unsaturated fatty acids with 20 up to as many as 30 carbon atoms. The longest fatty acids normally found in this bacterium contain 18-carbon atoms. These results suggest that the FAS from Vibrio sp. strain ABE-1 has potentially the ability to synthesize fatty acids with very long chains.
Morita
N
N
Department of Biology, Faculty of Science, Hokkaido University, Sapporo, Japan.
Okajima
N
N
Gotoh
M
M
Hayashi
H
H
Okuyama
H
H
Sasaki
S
S
D000213:Acyl Carrier Protein / Q000378:metabolism; D000214:Acyl Coenzyme A / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D004952:Esters; D064429:Fatty Acid Synthases / Q000187:drug effects / Q000378:metabolism*; D005227:Fatty Acids / Q000032:analysis; D008316:Malonyl Coenzyme A / Q000378:metabolism; D013876:Thiophenes / Q000494:pharmacology; D014733:Vibrio / Q000737:chemistry / Q000201:enzymology*
D000213:Acyl Carrier Protein; D000214:Acyl Coenzyme A; D000900:Anti-Bacterial Agents; D004952:Esters; D005227:Fatty Acids; D013876:Thiophenes; D008316:Malonyl Coenzyme A; C035068:thiolactomycin; D064429:Fatty Acid Synthases