Intracellular boron localization and uptake in cell cultures using imaging secondary ion mass spectrometry (ion microscopy) for neutron capture therapy for cancer.
Quantitative ion microscopy of freeze-fractured, freeze-dried cultured cells is a technique for single cell and subcellular elemental analysis. This review describes the technique and its usefulness in determining the uptake and subcellular distribution of the boron from boron neutron capture therapy drugs.
Bennett
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BD
Department of Chemistry, Cornell University, Ithaca, NY 14853.
Zha
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Gay
I
I
Morrison
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GH
D000818:Animals; D001896:Boron Compounds / Q000493:pharmacokinetics* / Q000627:therapeutic use; D015925:Cryopreservation; D013058:Mass Spectrometry / Q000379:methods*; D051379:Mice; D008853:Microscopy / Q000379:methods*; D009369:Neoplasms / Q000532:radiotherapy*; D009502:Neutrons; D010649:Phenylalanine / Q000031:analogs & derivatives* / Q000493:pharmacokinetics / Q000627:therapeutic use; D014407:Tumor Cells, Cultured; D014529:Uridine / Q000031:analogs & derivatives* / Q000493:pharmacokinetics / Q000627:therapeutic use
D001896:Boron Compounds; C076220:2'-O-(carboran-1-ylmethyl)uridine; D010649:Phenylalanine; C033685:4-boronophenylalanine; D014529:Uridine