Photodynamic therapy of the normal rat stomach: a comparative study between di-sulphonated aluminium phthalocyanine and 5-aminolaevulinic acid.
Dysplasia in the upper gastrointestinal tract carries a risk of invasive malignant change. Surgical excision of the affected organ is the only treatment available. Photodynamic therapy has been shown to be promising in the treatment of early and superficial tumours and may be useful for the ablation of dysplastic mucosa. Because of the diffuse nature of the disease, such treatment would necessarily involve destruction of large areas of mucosa and it is desirable to confine its effect to the mucosa in order that safe healing can take place. By means of photometric fluorescence microscopy, we have studied the pattern of photosensitisation in the normal rat stomach using di-sulphonated aluminium phthalocyanine (AlS2Pc) and 5-aminolaevulinic acid (ALA) as photosensitisizers. AlS2Pc resulted in a panmural photosensitisation of the gastric wall with the highest level encountered in the submucosa. The mucosa and muscularis propria were sensitised to equal extent. Following light exposure, a full thickness damage resulted. ALA is a natural porphyrin precursor and exogenous administration gave rise to accumulation of protoporphyrin IX (PPIX) in the cells. The resultant pattern of photosensitisation was predominantly mucosal and its photodynamic effect was essentially confined to the mucosa. ALA produced a selective photosensitisation of the gastric mucosa for its photodynamic ablation with sparing the underlying tissue layers.
Loh
C S
CS
Gastroenterology Unit, Walton Hospital, Liverpool, UK.
Bedwell
J
J
MacRobert
A J
AJ
Krasner
N
N
Phillips
D
D
Bown
S G
SG
D000622:Aminolevulinic Acid / Q000493:pharmacokinetics / Q000494:pharmacology*; D000818:Animals; D004305:Dose-Response Relationship, Drug; D005753:Gastric Mucosa / Q000378:metabolism; D007211:Indoles / Q000493:pharmacokinetics / Q000494:pharmacology*; D009336:Necrosis; D009942:Organometallic Compounds / Q000493:pharmacokinetics / Q000494:pharmacology*; D010778:Photochemotherapy; D051381:Rats; D011919:Rats, Inbred Strains; D013050:Spectrometry, Fluorescence; D013270:Stomach / Q000187:drug effects* / Q000473:pathology
D007211:Indoles; D009942:Organometallic Compounds; C060570:aluminum phthalocyanine; D000622:Aminolevulinic Acid