[The immunosuppressive effects of 0.025% cyclosporin eye drops in alpha cyclodextrin on rabbit corneal allografts].
We reported the ocular penetration of cyclosporin (CYA) and the immunosuppressive effect of rabbit corneal allograft using 0.025% CYA eye drops in alpha-cyclodextrin (alpha-CD). Local application using 0.025% CYA in alpha-CD showed the concentration of 4,133 ng/gr in the cornea, but no detectable levels in aqueous humor and serum. All eyes (10/10) in the CYA eye drop group remained clear for 100 days after corneal allografting. CYA eye drops halted and suppressed the corneal allograft's immune reaction when the treatment was begun early in the initial phase of rejection. These results indicate that 0.025% CYA eye drops in alpha-CD penetrate the cornea 5 to 10 times more than CYA eye drops in lipophilic vehicles. Furthermore, they are extremely effective in suppressing the immune reaction of corneal grafts.
Takano
T
T
Department of Ophthalmology, Juntendo University School of Medicine, Bunkyo-ku, Japan.
Kobayashi
C
C
Alba
R M
RM
Kanai
A
A
D000818:Animals; D003315:Cornea / Q000187:drug effects / Q000378:metabolism; D016039:Corneal Transplantation / Q000276:immunology*; D003505:Cyclodextrins / Q000008:administration & dosage / Q000494:pharmacology*; D016572:Cyclosporine / Q000493:pharmacokinetics / Q000494:pharmacology*; D006084:Graft Rejection / Q000187:drug effects; D007108:Immune Tolerance / Q000187:drug effects; D007165:Immunosuppression Therapy; D009883:Ophthalmic Solutions; D011817:Rabbits; D014183:Transplantation, Heterologous
D003505:Cyclodextrins; D009883:Ophthalmic Solutions; D016572:Cyclosporine