Improvement of blood compatibility on cellulose dialysis membrane. 2. Blood compatibility of phospholipid polymer grafted cellulose membrane.
The blood compatibility of a cellulose haemodialysis membrane whose surface was grafted with a methacrylate having a phospholipid polar group, 2-methacryloyloxyethyl phosphorylcholine, was evaluated with attention to platelet adhesion to the membrane surface and complement activation induced by the membrane. When the original cellulose membrane came in contact with platelet-rich plasma for 30 min, numerous platelets adhered to the surface and aggregated. On the other hand, the membrane grafted with 2-methacryloyloxyethyl phosphorylcholine effectively suppressed platelet adhesion and activation. This effect became more pronounced with increasing surface distribution. Especially, the 2-methacryloyloxyethyl phosphorylcholine grafted membranes, whose distribution exceeded 0.27, completely inhibited platelet adhesion, even when the contact time was 180 min. Moreover, the complement activation was also reduced with increased 2-methacryloyloxyethyl phosphorylcholine distribution on the surface of the membrane.
Ishihara
K
K
Institute for Medical and Dental Engineering, Tokyo Medical and Dental University, Japan.
Takayama
R
R
Nakabayashi
N
N
Fukumoto
K
K
Aoki
J
J
D000818:Animals; D001672:Biocompatible Materials; D001798:Blood Proteins / Q000187:drug effects / Q000378:metabolism; D002482:Cellulose / Q000737:chemistry / Q000494:pharmacology*; D003167:Complement Activation / Q000187:drug effects; D008567:Membranes, Artificial; D008689:Methacrylates / Q000737:chemistry / Q000494:pharmacology*; D010767:Phosphorylcholine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D010973:Platelet Adhesiveness / Q000187:drug effects; D011817:Rabbits; D006435:Renal Dialysis / Q000295:instrumentation*
D001672:Biocompatible Materials; D001798:Blood Proteins; D008567:Membranes, Artificial; D008689:Methacrylates; D010767:Phosphorylcholine; C070638:2-methacryloyloxyethyl phosphorylcholine; D002482:Cellulose