Cholesterol immobilization via ether-linked sepharose gels.
Cholesterol has been immobilized on Sepharose-6B via oxyether linkages to the 3- or 25-position. The 3- or 25-hydroxysterol methanesulfonates were coupled with epoxy-Sepharose-6B at 80 degrees C for 24 h. Approximately 2% of the ligand was incorporated into the gel. These types of affinity columns may be useful in purifying proteins that specifically bind or metabolize cholesterol.
Satsangi
R K
RK
Department of Pathology, University of Texas Health Science Center, San Antonio 78284.
Mott
G E
GE
D002784:Cholesterol / Q000737:chemistry / Q000302:isolation & purification*; D004987:Ethers; D005782:Gels; D012685:Sepharose
D004987:Ethers; D005782:Gels; D012685:Sepharose; D002784:Cholesterol