title: Localization of actin in normal human hepatocytes using fluorescent phallotoxins and immunohistochemical amplification.
abstract: Two different methods, fluorescent phallotoxins and immunohistochemical amplification systems were used to visualize actin in normal human hepatocytes. With fluorescent phallotoxins (NBD-phallacidin or rhodamine phalloidin), F-actin was distributed along the plasma membranes and at the bile canaliculi. With immunohistochemical methods (biotin-avidin, biotin-streptavidin, silver enhancement), actin was found at the same level, however a cytoplasmic staining was observed and discussed as G-actin localization.
authors.affiliation: Laboratoire d'Anatomie Pathologique et de Cytochimie, Faculté de Médecine, Marseille, France.
authors.forename: L
authors.initials: L
authors.lastname: Benkoel
authors.forename: J
authors.initials: J
authors.lastname: Brisse
authors.forename: C
authors.initials: C
authors.lastname: Capo
authors.forename: A M
authors.initials: AM
authors.lastname: Benoliel
authors.forename: P
authors.initials: P
authors.lastname: Bongrand
authors.forename: T
authors.initials: T
authors.lastname: Garcia
authors.forename: A
authors.initials: A
authors.lastname: Chamlian
mesh_terms: D000199:Actins / Q000032:analysis*; D000911:Antibodies, Monoclonal; D005456:Fluorescent Dyes; D006801:Humans; D007150:Immunohistochemistry; D008099:Liver / Q000737:chemistry* / Q000166:cytology; D010590:Phalloidine / Q000031:analogs & derivatives*
chemical_list: D000199:Actins; D000911:Antibodies, Monoclonal; D005456:Fluorescent Dyes; D010590:Phalloidine