In vivo sister chromatid differentiation and baseline sister chromatid exchanges in a mouse ascites tumour model.
Induction of differentially stained sister chromatids at G2/M and determination of baseline sister chromatid exchanges (SCEs) in ascites form of mouse sarcoma 180 cell line have been done by in vivo incorporation of 5-bromodeoxyuridine (BrdU) for two consecutive DNA replication cycles. The baseline SCE frequency is 6.24 at log phase of tumour growth.
Chakrabarti
A
A
Department of Zoology, Vivekananda College, Burdwan, India.
Acharya
S
S
Pal
A K
AK
Huda
R
R
Chakrabarti
S
S
D000818:Animals; D001973:Bromodeoxyuridine; D004305:Dose-Response Relationship, Drug; D051379:Mice; D012513:Sarcoma, Experimental / Q000235:genetics*; D012854:Sister Chromatid Exchange / Q000187:drug effects*
D001973:Bromodeoxyuridine