title: Molecular cloning of the NK1.1 antigen, a member of the NKR-P1 family of natural killer cell activation molecules.
abstract: In mice, the NK1.1 alloantigen is expressed on all NK cells and it initiates transmembrane signals that activate cytotoxicity. NK1.1 has been mapped to a chromosomal region that encodes two families of receptor-like molecules, the NKR-P1 family and the Ly-49 family. Both of these gene families encode type II integral membrane proteins whose extracellular domains are homologous with known C-type lectins. We have isolated and expressed a member of the NKR-P1 gene family (mNKR-P1.9) and have demonstrated both by immunofluorescence and by immunoprecipitation that this cDNA encodes NK1.1. These findings, which demonstrate the receptor-like structure of NK1.1, will facilitate studies regarding the role of NK1.1 in natural killing and regarding the identification of possible NK1.1 ligands.
authors.affiliation: Department of Medicine, University of California, San Francisco 94143.
authors.forename: J C
authors.initials: JC
authors.lastname: Ryan
authors.forename: J
authors.initials: J
authors.lastname: Turck
authors.forename: E C
authors.initials: EC
authors.lastname: Niemi
authors.forename: W M
authors.initials: WM
authors.lastname: Yokoyama
authors.forename: W E
authors.initials: WE
authors.lastname: Seaman
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000737:chemistry; D007519:Isoantigens / Q000032:analysis / Q000235:genetics* / Q000502:physiology; D007694:Killer Cells, Natural / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data
chemical_list: D007519:Isoantigens; D004247:DNA