A simple and economical strategy for downstream processing of specific antibodies to human transferrin from egg yolk.
A rapid and economical procedure for extraction of antibodies from egg yolk is described. Laying hens were immunized with human transferrin and extracts of egg-yolk were purified with a procedure based on affinity chromatography. The resulting purified antibodies were evaluated in a nephelometric system for the assay of transferrin in human sera. The results agreed closely with those obtained with a commercially available anti-human transferrin serum from rabbits.
Ntakarutimana
V
V
Laboratory of Medical Biochemistry, Universitaire Instelling Antwerpen (U.I.A.), Wilrijk, Belgium.
Demedts
P
P
van Sande
M
M
Scharpé
S
S
D000906:Antibodies / Q000302:isolation & purification*; D002846:Chromatography, Affinity; D004530:Egg Yolk / Q000276:immunology*; D006801:Humans; D007114:Immunization; D007136:Immunoglobulins / Q000302:isolation & purification; D009391:Nephelometry and Turbidimetry; D014168:Transferrin / Q000276:immunology*
D000906:Antibodies; C016512:IgY; D007136:Immunoglobulins; D014168:Transferrin