Obtaining mutants for protoplast fusion of gibberellin-forming Gibberella fujikuroi strains.
Auxotrophic, drug-resistant, nitrate-nonutilizing, and albino mutants have been isolated in Gibberella fujikuroi following UV mutagenesis. Protoplasts of complementing auxotrophic strains, mutants with resistance markers, or mutants blocked in different steps of the nitrate assimilatory pathway have been fused to form heterokaryons, diploids, or recombinant haploids. The properties of fusant strains, including gibberellic acid productivity, have been examined and compared to parent strains.
Torres
K B
KB
Friedrich Schiller University Jena, Institute of Microbiology, Germany.
Brückner
B
B
Meier
B
B
D002569:Cerulenin / Q000494:pharmacology; D004171:Diploidy; D004352:Drug Resistance, Microbial; D005874:Gibberella / Q000235:genetics*; D005875:Gibberellins / Q000096:biosynthesis*; D006238:Haploidy; D016296:Mutagenesis; D009761:Nystatin / Q000494:pharmacology; D011523:Protoplasts; D014466:Ultraviolet Rays
D005875:Gibberellins; D009761:Nystatin; D002569:Cerulenin