Methylation inhibits the interaction of DNA binding proteins with a potential c-abl intron regulatory element.
We have identified DNA binding proteins which interact with a sequence found in an intron of the tyrosine kinase coding portion of the murine c-abl gene. Several specific DNA: protein complexes were observed. Those complexes of approximate molecular weights 64 and 66kDa were detected when an Msp I site (CCGG) within the sequence was unmethylated, but were not observed when that site was methylated. Insertion of the intron sequence 5' to the rat somatic cytochrome C promoter and chloramphenicol acetyl transferase (CAT) sequences resulted in at least four-fold stimulation of CAT activity. These data suggest a potential role for the intron sequence in the regulation of gene expression.
Hengst-Zhang
J A
JA
Department of Medicine, Northwestern University Medical School, Chicago, Illinois 60611.
Weitzman
S A
SA
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000302:isolation & purification / Q000378:metabolism* / Q000528:radiation effects; D016313:Genes, abl / Q000528:radiation effects; D007438:Introns; D008745:Methylation; D051379:Mice; D008969:Molecular Sequence Data; D008970:Molecular Weight; D015345:Oligonucleotide Probes; D011505:Protein-Tyrosine Kinases / Q000235:genetics* / Q000378:metabolism; D016315:Proto-Oncogene Proteins c-abl / Q000235:genetics* / Q000378:metabolism; D012045:Regulatory Sequences, Nucleic Acid; D014162:Transfection; D014466:Ultraviolet Rays
D004268:DNA-Binding Proteins; D015345:Oligonucleotide Probes; D015500:Chloramphenicol O-Acetyltransferase; D011505:Protein-Tyrosine Kinases; D016315:Proto-Oncogene Proteins c-abl