title: The measurement of mitochondrial beta-oxidation by release of 3H2O from [9,10-3H]hexadecanoate: application to skeletal muscle and the use of inhibitors as models of metabolic disease.
abstract: We describe the use of a simple assay for beta-oxidation which depends on the release of 3H2O from [9,10-3H]hexadecanoate. This was compared with the use of [1-14C]hexadecanoate which gave comparable results when all the products of beta-oxidation were measured. The prediction that 75% of the tritium is released as 3H2O and 25% as [2-3H]acetyl units was confirmed. The assay was used successfully to demonstrate impaired beta-oxidation in tissue preparations from rats treated with etomoxir and methylenecyclopropylpyruvate which are known inhibitors of beta-oxidation. Abnormalities of beta-oxidation were also detected in skeletal muscle from patients with defects of mitochondrial oxidation.
authors.affiliation: Division of Clinical Neuroscience Medical School, University of Newcastle upon Tyne, United Kingdom.
authors.forename: R S
authors.initials: RS
authors.lastname: Kler
authors.forename: H S
authors.initials: HS
authors.lastname: Sherratt
authors.forename: D M
authors.initials: DM
authors.lastname: Turnbull
mesh_terms: D000818:Animals; D001792:Blood Platelets / Q000378:metabolism*; D002952:Citric Acid Cycle; D004852:Epoxy Compounds / Q000494:pharmacology*; D006801:Humans; D007004:Hypoglycemic Agents / Q000494:pharmacology*; D007005:Hypoglycins / Q000494:pharmacology*; D007700:Kinetics; D008297:Male; D008659:Metabolic Diseases / Q000378:metabolism*; D008930:Mitochondria, Liver / Q000187:drug effects / Q000378:metabolism*; D008931:Mitochondria, Muscle / Q000187:drug effects / Q000378:metabolism*; D008954:Models, Biological; D010084:Oxidation-Reduction; D010169:Palmitic Acids / Q000378:metabolism*; D011865:Radioisotope Dilution Technique; D051381:Rats; D011919:Rats, Inbred Strains; D014316:Tritium; D014867:Water
chemical_list: D004852:Epoxy Compounds; D007004:Hypoglycemic Agents; D007005:Hypoglycins; D010169:Palmitic Acids; D014867:Water; D014316:Tritium; C022149:ketohypoglycin; C054207:etomoxir