title: [Anti-C100-3 antibody titration using 4-parameter logistic curve (results in its application to hepatitis C cases treated with interferon].
abstract: We evaluated titers of C100-3 antibody by ELISA using a 4-parameter logistic fitting curve and examined the transition of the values in the cases of interferon treatment. The 4-parameter logistic fitting curve produced a standard curve in a wider range using a micro plate reader than the logarithmic curve. The values of C100-3 antibody during and after the treatments of interferon were changed in parallel with the alterations of ALT. The anti-C100-3 antibody titers assayed in the complete or partial responders were significantly reduced at the end of interferon therapy. These findings suggest the clinical usefulness of the evaluation of anti C100-3 antibody titers.
authors.affiliation: Clinical Laboratory, National Osaka Hospital.
authors.forename: T
authors.initials: T
authors.lastname: Nakagawa
authors.forename: K
authors.initials: K
authors.lastname: Enoki
authors.forename: S
authors.initials: S
authors.lastname: Shimamoto
authors.forename: T
authors.initials: T
authors.lastname: Hishimoto
authors.forename: A
authors.initials: A
authors.lastname: Kurata
mesh_terms: D000956:Antigens, Viral; D004797:Enzyme-Linked Immunosorbent Assay; D006508:Hepatitis Antibodies / Q000032:analysis*; D006526:Hepatitis C / Q000175:diagnosis / Q000628:therapy*; D006801:Humans; D016898:Interferon-alpha / Q000627:therapeutic use*; D017361:Viral Nonstructural Proteins; D014764:Viral Proteins / Q000276:immunology*
chemical_list: D000956:Antigens, Viral; C064544:C100 protein, hepatitis C virus; D006508:Hepatitis Antibodies; D016898:Interferon-alpha; D017361:Viral Nonstructural Proteins; D014764:Viral Proteins