Diagnostic application of monoclonal antibodies to outer membrane protein for rapid detection of Salmonella.
Monoclonal antibodies produced to Salmonella enteritidis outer membrane proteins were screened against 57 Salmonella serovars and several related enterobacteria. Those detecting all Salmonella serovars and none of the related enterobacteria were used in a microtitre plate antigen capture ELISA to screen clinical samples. Sixty-one of 2100 samples yielded salmonellas after incubation for 24 h in selective media by conventional culture. Of these 58 were detected by the ELISA. Sixty-five false positives by ELISA were found to be Enterobacter spp. The results show the potential of this ELISA to eliminate a large proportion of the salmonella-negative cultures at an early stage.
Kerr
S
S
Bacteriology Department, Veterinary Sciences Division, Stormont, Belfast, UK.
Ball
H J
HJ
Mackie
D P
DP
Pollock
D A
DA
Finlay
D A
DA
D000911:Antibodies, Monoclonal; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology*; D003429:Cross Reactions; D004755:Enterobacteriaceae / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005188:False Negative Reactions; D012475:Salmonella / Q000145:classification / Q000276:immunology / Q000302:isolation & purification*; D012477:Salmonella enteritidis / Q000145:classification / Q000276:immunology / Q000302:isolation & purification; D012486:Salmonella typhimurium / Q000145:classification / Q000276:immunology / Q000302:isolation & purification; D012680:Sensitivity and Specificity; D012703:Serotyping
D000911:Antibodies, Monoclonal; D001425:Bacterial Outer Membrane Proteins