Molecular analysis of a cruciferin storage protein gene family of Brassica napus.
We have isolated a five-member gene subfamily which encodes cruciferin, a legumin-like 12S storage protein of Brassica napus L., and have analyzed the structure and expression of the family members in developing embryos. Sequence analysis has shown that the coding regions of all five genes are highly similar, with the two most divergent members of the family retaining 89% sequence identity. The analysis of this cruciferin gene family's expression indicates that the developmental pattern of expression of each gene is similar, and the steady-state mRNA levels of each gene are approximately equivalent to each other at all developmental stages.
Breen
J P
JP
Biology Department, Indiana University, Bloomington 47405.
Crouch
M L
ML
D000485:Allergens; D052179:Antigens, Plant; D001483:Base Sequence; D001937:Brassica / Q000235:genetics*; D004247:DNA; D015870:Gene Expression; D008969:Molecular Sequence Data; D005810:Multigene Family; D010940:Plant Proteins / Q000235:genetics*; D015183:Restriction Mapping; D055314:Seed Storage Proteins; D012689:Sequence Homology, Nucleic Acid
D000485:Allergens; D052179:Antigens, Plant; D010940:Plant Proteins; D055314:Seed Storage Proteins; C059777:cruciferin protein, Brassica napus; D004247:DNA