[Immunophenotypic analysis in acute leukemia].
Acute leukemias have been classified on French-American-British (FAB) criteria depending on the morphocytochemical features of blasts. Immunophenotyping and clonal rearrangement analysis of lineage-associated genes can decide a frozen stage of the hematopoietic differentiation process in blasts from acute leukemias. B-lineage acute lymphoblastic leukemia (ALL) and T-lineage ALL are systematically classified according to the sequential expression of differentiation-associated antigens. In acute myelogenous leukemia (AML), several new entities are proposed: AML-M0 is an AML without cytologic maturation, in which the myeloid commitment should be demonstrated by myeloperoxidase-positive microgranule on immunohistochemical staining or electron-microscopy, or by positive reaction for CD13 or CD33 antigens. CD7-positive AML is considered to be one of immature subtypes of AML, rather than hybrid leukemia. Thus, immunological studies on blasts enable us to discriminate a subgroup of leukemias, which will perhaps contribute to the improvement of treatment approach.
Akashi
K
K
First Department of Internal Medicine, Faculty of Medicine, Kyushu University.
Harada
M
M
D000208:Acute Disease; D015703:Antigens, CD / Q000032:analysis*; D000954:Antigens, Surface / Q000032:analysis*; D006801:Humans; D016130:Immunophenotyping; D007938:Leukemia / Q000145:classification* / Q000276:immunology / Q000473:pathology; D008214:Lymphocytes / Q000473:pathology
D015703:Antigens, CD; D000954:Antigens, Surface