HPLC separation of 32P-postlabelled DNA adducts formed from dibenz[a,h]anthracene in skin.
Mouse skin and human skin have been treated in vivo or in short-term organ culture with dibenz[a,h]anthracene (DB[a,h]A), the related 3,4- or 5,6-diols or the anti- or syn-3,4-diol 1,2-oxides. DNA hydrolysates have been 32P-postlabelled and the adducts present examined by HPLC using a phenyl-modified reverse phase column and, for comparison, by PEI-cellulose TLC and autoradiography. The adducts formed when the diol-epoxides were reacted with salmon sperm DNA were also examined. The results show that in mouse skin treated in vivo, the major adducts formed from DB[a,h]A and the 3,4-diol were the same and that two of them were more polar than those formed in skin or in DNA that had been treated with the related anti- or syn-diol epoxides. Human skin treated with DB[a,h]A in culture yielded an adduct profile that was qualitatively similar to the profiles obtained with mouse skin.
Lecoq
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S
Haddow Laboratories, Institute of Cancer Research, Sutton, Surrey, UK.
Pfau
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W
Grover
P L
PL
Phillips
D H
DH
D000818:Animals; D001551:Benz(a)Anthracenes / Q000302:isolation & purification* / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000302:isolation & purification* / Q000378:metabolism; D006801:Humans; D007553:Isotope Labeling; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009924:Organ Culture Techniques; D010761:Phosphorus Radioisotopes; D012867:Skin / Q000737:chemistry*
D001551:Benz(a)Anthracenes; D010761:Phosphorus Radioisotopes; C057927:dibenz(a,j)anthracene-3,4-diol-1,2-epoxide; C018913:dibenzoanthracene-5,6-dihydrodiol; C079612:dibenz(a,h)anthracene-3,4-diol; C079613:dibenz(a,h)anthracene-3,4-diol 1,2-oxide; D004247:DNA; C026486:1,2,5,6-dibenzanthracene