Tissue-specific expression of type XIV collagen--a member of the FACIT class of collagens.
The collagens represent a highly diverse superfamily of extracellular matrix proteins that can be divided into several distinct families. One of the families, called FACIT (fibril-associated collagens with interrupted triple-helices) family, contains molecules that appear to be associated with cross-striated fibrils composed of members of the fibrillar collagen family. We have determined a portion of the primary structure of a recently discovered member of the FACIT family, chicken alpha 1(XIV) collagen, based on cloning and sequencing cDNAs. A synthetic oligopeptide from within the carboxy-terminal non-triple-helical domain of the alpha 1(XIV) chain has been used for generating specific polyclonal antibodies. The antiserum, PS1, recognizes a 220 kDa polypeptide in immunoblots of extracts of chicken skin, tendons, and cartilage. Sequencing of a tryptic peptide generated from purified, immunoreactive material, gives a sequence identical to that derived from cDNA sequencing, providing strong support for the type XIV-specificity of PS1. We have examined the expression of type XIV collagen in developing chick embryos by immunostaining of sections from 12-day-old embryos with PS1 and by Northern blot analysis of RNA from several tissues from both 12- and 17-day-old embryos. The results show that type XIV collagen is prevalent within relatively dense connective tissues such as dermis, tendons, perichondrium, perimysium, the stroma of lungs and liver, and blood vessels.
Castagnola
P
P
Istituto Nazionale per la Ricerca sul Cancro, Genova/Italy.
Tavella
S
S
Gerecke
D R
DR
Dublet
B
B
Gordon
M K
MK
Seyer
J
J
Cancedda
R
R
van der Rest
M
M
Olsen
B R
BR
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002645:Chickens; D003094:Collagen / Q000032:analysis* / Q000235:genetics; D003238:Connective Tissue / Q000737:chemistry*; D004247:DNA / Q000302:isolation & purification; D006023:Glycoproteins / Q000032:analysis* / Q000235:genetics; D015151:Immunoblotting; D007124:Immunoenzyme Techniques; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000138:chemical synthesis; D012680:Sensitivity and Specificity; D017386:Sequence Homology, Amino Acid; D012867:Skin / Q000737:chemistry; D014357:Trypsin
C054447:COL14A1 protein, human; D006023:Glycoproteins; D009842:Oligopeptides; D003094:Collagen; D004247:DNA; D014357:Trypsin