[Evaluation of enzyme-linked immunosorbent assay for serodiagnosis of Bordetella bronchiseptica infection in guinea pigs].
An enzyme-linked immunosorbent assay (ELISA) for serodiagnosis of Bordetella bronchiseptica (B. bronchiseptica) infection in guinea pigs was evaluated. An isolate of B. bronchiseptica from lung lesion of a guinea pig was used as antigen after ultrasonication. In the experimental infection, specific pathogen-free guinea pigs inoculated with the bacterium intranasally were examined every 5 or 10 days. The organism was recovered from all animals between 5 and 30 days post-inoculation (p.i.). Only one animal was sero-positive by agglutination test 30 and 50 days p.i.; whereas, by ELISA, one animal was positive 5 days p.i., and all the animals showed strong reaction 20 to 50 (end of experiment) days p.i. Field samples obtained from 1983 to 1989 were tested by ELISA. The results corresponded to those of macroscopic observations and bacterial isolation. The ELISA proved to be useful method for detection of B. bronchiseptica infection in guinea pigs.
Mikazuki
K
K
Aburahi Laboratories, Shionogi Pharmaceutical Co., Ltd., Shiga, Japan.
Hirasawa
T
T
Sakai
Y
Y
Ohhara
S
S
Nenui
H
H
Takahashi
K
K
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis; D001885:Bordetella Infections / Q000175:diagnosis* / Q000276:immunology; D016950:Bordetella bronchiseptica / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D006168:Guinea Pigs; D008297:Male; D011237:Predictive Value of Tests; D012698:Serologic Tests
D000907:Antibodies, Bacterial