Identification of aerobactin genes in clinical isolates of E. coli using a non-radioactive DNA probe.
A digoxigenin-labelled gene probe was used for the identification of aerobactin genes in 21 E. coli strains from clinical isolates by means of a colony hybridization test. The results were compared with those obtained by previous hybridization experiments with radiolabelled DNA probes as well as with a crossfeeding-assay. It could be demonstrated that only after an additional proteinase K treatment of the filters before hybridization and after a repeated blocking during the immunological detection procedure, specific hybridization signals could be observed.
Gräser
Y
Y
Institut für Medizinische Mikrobiologie, Bereiches Medizin (Charité), Humboldt-Universität, Berlin, Germany.
Schönian
G
G
D015342:DNA Probes / Q000737:chemistry*; D004076:Digoxigenin / Q000737:chemistry; D004926:Escherichia coli / Q000737:chemistry / Q000235:genetics*; D004927:Escherichia coli Infections / Q000235:genetics; D005798:Genes, Bacterial / Q000235:genetics*; D006801:Humans; D006877:Hydroxamic Acids / Q000737:chemistry*; D009693:Nucleic Acid Hybridization
D015342:DNA Probes; D006877:Hydroxamic Acids; C031819:aerobactin; D004076:Digoxigenin