Effects of acrylamide on germinal vesicle migration and dissolution in oocytes of the frog, Rana pipiens.
We have demonstrated that when Rana oocytes are treated with 10 mM acrylamide, germinal vesicle migration (GVM) is promoted while meiosis reinitiation by progesterone is inhibited. A number of other specific alterations result from the acrylamide treatment: (i) A dense band of fibrillar material appears adjacent to the oolemma in acrylamide-treated oocytes. Furthermore, (ii) the fibrillar material reacts with an intermediate filament antibody using immunogold techniques applied to transmission electron microscopy. Moreover, (iii) acrylamide inhibits progestogen-induced annulate lamellae breakdown. In addition, (iv) the cortical mitochondria-rich layer appears to be thickened by acrylamide, which also (v) affects oocyte microvillar retraction and organization. Finally, (vi) electrophysiological measurement of membrane voltage indicates that acrylamide does not significantly affect cell viability during the incubation period used in this study. In summary, acrylamide exerts profound effects on the physiological event of GVM, and these are consistent with the hypothesis that changes in the cytoskeleton are a contributing factor in meiosis reinitiation.
Lessman
C A
CA
Department of Biology, Memphis State University, Tennessee 38152.
Kessel
R G
RG
D020106:Acrylamide; D000178:Acrylamides / Q000494:pharmacology*; D008841:Actin Cytoskeleton / Q000187:drug effects / Q000648:ultrastructure; D000818:Animals; D002465:Cell Movement / Q000187:drug effects; D002478:Cells, Cultured; D005260:Female; D007382:Intermediate Filaments / Q000187:drug effects / Q000648:ultrastructure*; D007700:Kinetics; D008540:Meiosis / Q000187:drug effects*; D008564:Membrane Potentials / Q000187:drug effects; D008839:Microelectrodes; D016253:Microscopy, Immunoelectron; D008871:Microvilli / Q000187:drug effects / Q000648:ultrastructure; D009865:Oocytes / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D006080:Ovarian Follicle / Q000166:cytology / Q000502:physiology*; D011374:Progesterone / Q000494:pharmacology; D011894:Rana pipiens
D000178:Acrylamides; D020106:Acrylamide; D011374:Progesterone