Microvascular changes of the liver preserved in UW solution. Pathological and immunohistochemical examination.
Rat livers preserved in University of Wisconsin (UW) solution for 24 h were compared with those preserved in Euro-Collins (EC) solution before and after liver transplantation using an immunohistochemical method. Tissue ATP and total tissue adenine nucleotide (TAN) were measured using HPLC. The levels of TAN in the UW group or the EC group were significantly low compared with the control group (no preservation) after 24-h storage. In the EC group, the levels of tissue adenine nucleotides (TAN) decreased 1 h after reperfusion and never reached control levels. In the UW group, the levels of TAN increased a little 1 h after reperfusion and increased more 3 h after reperfusion. After 24-h preservation, the expression of factor VIII-related antigen (FRA) in endothelial cells of central veins was weak in the EC group; in the UW group, FRA was clearly detected in these cells. After reperfusion, although severe endothelial cell damage to the central veins and numerous FRA-positive substances were observed in EC group, endothelial cells of central veins retained their normal structure and FRA-positive substances were rarely noted in the UW group. In both groups, no endothelial changes were detected in portal veins. From these results, it is concluded that UW solution prevents endothelial cell damage and microcirculatory injury in zone III during the preservation period resulting in prevention of initial graft nonfunction. Also, measurement of the TAN level after reperfusion is useful to predict the function of the graft.
Haba
T
T
Department of Surgery II, Nagoya University School of Medicine, Japan.
Hayashi
S
S
Hachisuka
T
T
Ootsuka
S
S
Tanaka
Y
Y
Satou
E
E
Takagi
H
H
D000227:Adenine Nucleotides / Q000378:metabolism; D000241:Adenosine; D000493:Allopurinol; D000818:Animals; D005978:Glutathione; D006982:Hypertonic Solutions; D007150:Immunohistochemistry; D007328:Insulin; D008099:Liver / Q000098:blood supply / Q000378:metabolism / Q000473:pathology; D016031:Liver Transplantation; D008297:Male; D008833:Microcirculation / Q000378:metabolism / Q000473:pathology; D009926:Organ Preservation / Q000379:methods*; D019188:Organ Preservation Solutions; D011887:Raffinose; D051381:Rats; D011919:Rats, Inbred Strains; D012996:Solutions; D014841:von Willebrand Factor / Q000378:metabolism
D000227:Adenine Nucleotides; C037096:Euro-Collins' solution; D006982:Hypertonic Solutions; D007328:Insulin; D019188:Organ Preservation Solutions; D012996:Solutions; C055206:University of Wisconsin-lactobionate solution; D014841:von Willebrand Factor; D000493:Allopurinol; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose