title: A gene encoding a superoxide dismutase of the facultative intracellular bacterium Listeria monocytogenes.
abstract: A gene (lmsod) encoding superoxide dismutase (SOD; EC 1.15.1.1) of the facultative intracellular pathogen, Listeria monocytogenes, was cloned by functional complementation of an SOD-deficient Escherichia coli mutant. The nucleotide sequence was determined and the deduced amino acid (aa) sequence (202 aa) showed close similarity to manganese-containing SOD's from other organisms. Subunits of the recombinant L. monocytogenes SOD (re-SOD) and of both E. coli SODs formed enzymatically active hybrid enzymes in vivo. DNA/DNA-hybridization experiments showed that this type of recombinant re-sod gene is conserved within the genus Listeria.
authors.affiliation: Theodor-Boveri-Institut für Biowissenschaften, Biozentrum, Universität Würzburg, Germany.
authors.forename: K
authors.initials: K
authors.lastname: Brehm
authors.forename: A
authors.initials: A
authors.lastname: Haas
authors.forename: W
authors.initials: W
authors.lastname: Goebel
authors.forename: J
authors.initials: J
authors.lastname: Kreft
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial / Q000235:genetics*; D005816:Genetic Complementation Test; D008089:Listeria monocytogenes / Q000201:enzymology / Q000235:genetics*; D008345:Manganese; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D012689:Sequence Homology, Nucleic Acid; D013482:Superoxide Dismutase / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial; D008345:Manganese; D013482:Superoxide Dismutase