Interaction of DNA with cationic liposomes: ability of transfecting lentil protoplasts.
The vesicle made of dipalmitoylphosphatidylcholine and stearylamine (9:1) were multilamellar and rather homogeneous in shape as seen by transmission electron microscopy. Upon addition of circular DNA plasmids of different lengths to the liposomes, the formation of vesicle clusters around the DNA filament was observed, with dimensions dictated by the ratio DNA/lipid. These liposomes were able to transfect lentil (Lens culinaris) protoplasts inside the cells two different reporter genes, chloramphenicol-acetyltransferase and beta-glucuronidase. The activity of these two enzymes could be found in the cell lysates after 24 h from the incubation of protoplasts with the lipid-DNA complexes.
Maccarrone
M
M
Dipartimenti di Medicina Sperimentale e Scienze Biochimiche e, Università di Roma Tor Vergata, Italy.
Dini
L
L
Di Marzio
L
L
Di Giulio
A
A
Rossi
A
A
Mossa
G
G
Finazzi-Agrò
A
A
D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics* / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics*; D004337:Drug Carriers; D004926:Escherichia coli / Q000235:genetics; D007887:Fabaceae / Q000235:genetics*; D005966:Glucuronidase / Q000235:genetics* / Q000378:metabolism; D008081:Liposomes; D008854:Microscopy, Electron; D010946:Plants, Medicinal; D010957:Plasmids; D011523:Protoplasts / Q000502:physiology; D014162:Transfection
D004269:DNA, Bacterial; D004337:Drug Carriers; D008081:Liposomes; D015500:Chloramphenicol O-Acetyltransferase; D005966:Glucuronidase