Simultaneous determination of cortisol and cortisone in human plasma by stable-isotope dilution mass spectrometry.
A method for the simultaneous determination of cortisol and cortisone in human plasma was developed using capillary gas chromatography-mass spectrometry-selected ion monitoring. [2H5]Cortisol and [2H5]cortisone were used as internal standards. Cortisol and cortisone in plasma were determined from the peak-height ratios of the [M-31] fragment ions of the methoxime-trimethylsilyl derivatives of cortisol and [2H5]cortisol (m/z 605 and 610) and of cortisone and [2H5]cortisone (m/z 531 and 536). Sensitivity, specificity, precision, accuracy and reproducibility of the method were demonstrated to be satisfactory for measuring the circulating concentrations of cortisol and cortisone.
Shibasaki
H
H
Department of Medicinal Chemistry and Clinical Pharmacy, Tokyo College of Pharmacy, Japan.
Arai
I
I
Furuta
T
T
Kasuya
Y
Y
D003348:Cortisone / Q000097:blood* / Q000302:isolation & purification; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D006854:Hydrocortisone / Q000097:blood* / Q000302:isolation & purification; D007202:Indicators and Reagents; D013058:Mass Spectrometry
D007202:Indicators and Reagents; D003348:Cortisone; D006854:Hydrocortisone