A double-label study demonstrating that all serotonin-like immunoreactive amacrine cells in the larval tiger salamander retina express GABA-like immunoreactivity.
A previous study localized serotonin-like immunoreactivity to amacrine cell populations in the larval tiger salamander retina. The present double-label immunocytochemical analysis of the tiger salamander retina was performed to determine if gamma-aminobutyric acid (GABA)-like immunoreactivity is expressed by serotonin-immunoreactive amacrine cells. More than 3,000 serotonin-amacrine cells were observed in double-label preparations, and all were found to express GABA-like immunoreactivity. This finding extends previous studies of serotonin-GABA coexistence in the retina by providing the first report of the co-localization of endogenous serotonin and GABA-like compounds in a retinal neuron.
Watt
C B
CB
Alice R. McPherson Laboratory of Retina Research, Center for Biotechnology, Baylor College of Medicine, The Woodlands, TX 77381.
D000818:Animals; D000906:Antibodies; D016650:Fluorescein-5-isothiocyanate; D005455:Fluorescent Antibody Technique; D008856:Microscopy, Fluorescence; D012160:Retina / Q000166:cytology*; D012701:Serotonin / Q000032:analysis*; D014562:Caudata; D005680:gamma-Aminobutyric Acid / Q000032:analysis*
D000906:Antibodies; D012701:Serotonin; D005680:gamma-Aminobutyric Acid; D016650:Fluorescein-5-isothiocyanate