Molecular characterization of the human immunoglobulin V lambda I germline gene repertoire.
To advance our understanding of the human immunoglobulin V lambda germline gene contribution to normal as well as autoimmune responses, we have isolated and sequenced six germline genes of the V lambda I subgroup. These genes can be divided into three sub-subgroups on the basis of greater than or equal to 93% nucleotide sequence homology and greater than or equal to 88% deduced amino acid sequence similarity. Examination of all cDNA and protein sequences available for expressed V lambda I genes supports the assignment of these three sub-subgroups. Sequence comparisons also suggest that germline gene members of two of these sub-subgroups, I-a and I-b, are preferentially utilized in the expressed V lambda I repertoire. This finding may be at least partially attributable to regulatory sequence abnormalities apparent in two of the other V lambda I germline genes (Humlv101 and Humlv104) which may interfere with their expression.
Daley
M D
MD
Department of Anaesthesia, University of Toronto, Ontario, Canada.
Olee
T
T
Peng
H Q
HQ
Soto-Gil
R W
RW
Chen
P P
PP
Siminovitch
K A
KA
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D015342:DNA Probes; D015723:Gene Library; D015894:Genome, Human; D006801:Humans; D007135:Immunoglobulin Variable Region / Q000235:genetics* / Q000302:isolation & purification; D007146:Immunoglobulin lambda-Chains / Q000235:genetics* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
D015342:DNA Probes; D007135:Immunoglobulin Variable Region; D007146:Immunoglobulin lambda-Chains