Transforming growth factor-beta-like activity is secreted by rabbit renal cortical tubular cells in primary culture.
The activity of an autocrine growth factor in a medium conditioned by cultured rabbit renal cortical tubular cells was investigated. Little stimulatory growth activity for tubular cells was observed in the conditioned medium, and inhibitory activity was seen only in acidified conditioned medium. This factor stimulated the colony formation of NRK 49F cells in soft agar only with epidermal growth factor and inhibited the DNA synthesis of primary cultured rat hepatocytes, and its molecular weight was about 25 kDa. The factor was neutralized by the specific antibody to transforming growth factor (TGF)-beta 1. These results indicate that renal tubular epithelial cells can produce latent TGF-beta in primary culture.
Kanda
S
S
Department of Urology, Nagasaki University School of Medicine, Japan.
Igawa
T
T
Taide
M
M
Nomata
K
K
Kanetake
H
H
Saito
Y
Y
D000818:Animals; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D004815:Epidermal Growth Factor / Q000494:pharmacology; D007328:Insulin / Q000494:pharmacology; D007672:Kidney Cortex / Q000187:drug effects / Q000378:metabolism; D007684:Kidney Tubules / Q000187:drug effects / Q000378:metabolism*; D011817:Rabbits; D016212:Transforming Growth Factor beta / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000378:metabolism*
D007328:Insulin; D016212:Transforming Growth Factor beta; D004815:Epidermal Growth Factor; D004247:DNA