Evaluation of a urease-based confirmatory enzyme-linked immunosorbent assay for diagnosis of Neisseria gonorrhoeae.
A new urease-based enzyme-linked immunosorbent assay utilizing novel monoclonal antibodies was evaluated for the culture confirmation of Neisseria gonorrhoeae, with 270 isolates of N. gonorrhoeae, 56 isolates of diverse Neisseria spp., and 29 Moraxella isolates. The test was highly specific (100.00%) and sensitive (97.83%). No cross-reactions were observed with any of the Neisseria or Moraxella isolates tested. Fifty percent (3 of 6) of the false-negative results were obtained with isolates of serovar IA-4, a serovar rarely encountered in North America.
Carballo
M
M
National Laboratory for Sexually Transmitted Diseases, Ottawa, Ontario, Canada.
Dillon
J R
JR
Lussier
M
M
Milthorp
P
P
Winston
S
S
Brodeur
B
B
D000911:Antibodies, Monoclonal; D001431:Bacteriological Techniques; D003951:Diagnostic Errors; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods* / Q000706:statistics & numerical data; D005069:Evaluation Studies as Topic; D006069:Gonorrhea / Q000175:diagnosis*; D006801:Humans; D009344:Neisseria gonorrhoeae / Q000276:immunology / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D014510:Urease
D000911:Antibodies, Monoclonal; D014510:Urease