title: Changes in bioactive lipids, alkylacylglycerol and ceramide, occur in HIV-infected cells.
abstract: The mass levels of bioactive lipids known to modulate signal transduction or to possess other biological activities were measured in HIV-infected CEM cells. The levels of diacylglycerol, an activator of protein kinase C, as well as of alkylacylglycerol were elevated. A more drastic increase was observed in the ceramide levels after HIV-infection, whereas sphingosine levels were hardly influenced. Interestingly, the magnitude of the changes was related to the infection time, being higher at 8 days after infection then at 4 days. The possible role of these lipids in the cytopathic effects of HIV-infection is discussed. In addition, an improved methodology to quantitate simultaneously diacylglycerol and alkylacylglycerol in crude lipid extracts, based upon their phosphorylation by E. coli diacylglycerol kinase, is presented.
authors.affiliation: Katolieke Universiteit Leuven, Faculty of Medicine, Department of Pharmacology, Belgium.
authors.forename: P P
authors.initials: PP
authors.lastname: Van Veldhoven
authors.forename: T J
authors.initials: TJ
authors.lastname: Matthews
authors.forename: D P
authors.initials: DP
authors.lastname: Bolognesi
authors.forename: R M
authors.initials: RM
authors.lastname: Bell
mesh_terms: D002460:Cell Line; D002518:Ceramides / Q000378:metabolism*; D004075:Diglycerides / Q000378:metabolism; D005989:Glycerides / Q000378:metabolism*; D005995:Glyceryl Ethers; D006678:HIV / Q000502:physiology*; D007700:Kinetics; D050356:Lipid Metabolism; D015398:Signal Transduction
chemical_list: C011439:1,2-diacylglycerol; D002518:Ceramides; D004075:Diglycerides; D005989:Glycerides; D005995:Glyceryl Ethers