Localization of cytochrome P-450 gene expression in normal and diseased human liver by in situ hybridization of wax-embedded archival material.
The localization of the expression of several cytochrome P-450 genes in normal and diseased human liver was investigated by in situ hybridization of formalin-fixed, paraffin wax-embedded archival tissue samples with 35S-labeled antisense RNA probes. The results demonstrated that genes coding for members of the cytochrome P-450 3A subfamily (CYP3A) were preferentially expressed in hepatocytes in acinar zone 3 (the centrilobular region), whereas genes coding for CYP1A2, CYP2A, 2B and 2C were expressed uniformly throughout the liver acinus. In cirrhotic livers, CYP2A and 2B genes (and to a lesser extent, CYP3A genes) were highly expressed in isolated hepatocytes located at the junction of parenchyma with fibrous septa. The cause and significance of the position-dependent expression of specific cytochrome P-450 genes in normal and diseased human liver are discussed.
Palmer
C N
CN
Department of Biochemistry, Queen Mary and Westfield College, University of London, United Kingdom.
Coates
P J
PJ
Davies
S E
SE
Shephard
E A
EA
Phillips
I R
IR
D003577:Cytochrome P-450 Enzyme System / Q000145:classification / Q000235:genetics*; D019286:Endopeptidase K; D015870:Gene Expression; D006801:Humans; D008099:Liver / Q000737:chemistry* / Q000473:pathology; D008103:Liver Cirrhosis / Q000235:genetics* / Q000473:pathology; D009693:Nucleic Acid Hybridization; D015347:RNA Probes; D012333:RNA, Messenger / Q000032:analysis*; D012697:Serine Endopeptidases; D016610:Tissue Embedding
D015347:RNA Probes; D012333:RNA, Messenger; D003577:Cytochrome P-450 Enzyme System; D012697:Serine Endopeptidases; D019286:Endopeptidase K