Detection of lipopolysaccharide (LPS) and identification of its serotype by an enzyme-linked immunosorbent assay (ELISA) using poly-L-lysine.
A new solid-phase enzyme-linked immunosorbent assay (ELISA) was developed for detection of LPS and identification of its serotype with antisera. Since LPS binds poorly to polystyrene microplates, precoating with poly-L-lysine was used before coating LPS on the surface of microplates. The small amount of LPS in complex mixtures (i.e., less than 1 microgram/ml) could be detectable in ELISA. Use of poly-L-lysine with high molecular weight (MW) provided a higher sensitivity than poly-L-lysine with low MW. Precoating with polymyxin B, or poly-L-histidine was less effective in the sensitivity than precoating with poly-L-lysine, but it was still better than no precoating. The newly developed ELISA technique could be also applied for detection of anti-LPS antibodies in sera or for screening of monoclonal anti-LPS antibody.
Takahashi
K
K
Department of Microbiology, Aichi Medical University School of Medicine, Japan.
Fukada
M
M
Kawai
M
M
Yokochi
T
T
D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay; D008050:Lipid A / Q000032:analysis; D008070:Lipopolysaccharides / Q000032:analysis* / Q000276:immunology; D008970:Molecular Weight; D011107:Polylysine; D011817:Rabbits
D008050:Lipid A; D008070:Lipopolysaccharides; D011107:Polylysine