title: Replacement of m-calpain by mu-calpain during maturation of megakaryocytes and possible involvement in platelet formation.
abstract: Localization of calpains in human bone marrow cells was studied immunohistochemically employing monoclonal antibodies against the high-Ca(2+)-requiring form (m-calpain) and the low-Ca(2+)-requiring form (mu-calpain). Most cells were stained with anti-m-calpain more strongly than with anti-mu-calpain, and staining with anti-mu-calpain was prominent only in megakaryocytes. To confirm the result, megakaryoblastic cell line (T-33) cells were subjected to immunoblot analysis. However no immunoreactivity to mu-calpain was seen in T-33 cells. Bone marrow from a patient with idiopathic thrombocytopenic purpura showed immature megakaryocytes (stage II) strongly stained by anti-m-calpain antibody while mature cells (stage III) were strongly stained by anti-mu-calpain antibody. These results suggest that mu-calpain plays a crucial role in mature megakaryocytes, possibly in platelet production.
authors.affiliation: Department of Biochemistry, Tokyo Metropolitan Institute of Gerontology, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Nakamura
authors.forename: M
authors.initials: M
authors.lastname: Mori
authors.forename: S
authors.initials: S
authors.lastname: Nakazawa
authors.forename: T
authors.initials: T
authors.lastname: Tange
authors.forename: M
authors.initials: M
authors.lastname: Hayashi
authors.forename: Y
authors.initials: Y
authors.lastname: Saito
authors.forename: S
authors.initials: S
authors.lastname: Kawashima
mesh_terms: D000911:Antibodies, Monoclonal; D001853:Bone Marrow / Q000737:chemistry*; D002154:Calpain / Q000032:analysis* / Q000737:chemistry; D002460:Cell Line / Q000737:chemistry; D006410:Hematopoiesis; D006801:Humans; D007150:Immunohistochemistry; D008533:Megakaryocytes / Q000737:chemistry* / Q000166:cytology; D016553:Purpura, Thrombocytopenic, Idiopathic / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D002154:Calpain