title: Murine monoclonal antibodies against staphylococcal enterotoxin B: production and characterization.
abstract: A group of 14 monoclonal antibodies (mAbs) to staphylococcal enterotoxin B (SEB) were obtained by fusion of Sp2/O myeloma cells with spleen cells from female BALB/c mice immunized with commercial SEB. The antibodies belonged to IgG1 and IgG2b subclasses. We evaluated the anti-SEB titres, competition assays and sensitivity of detection by indirect ELISA. Reactivity and cross-reactivity were also studied by indirect ELISA and confirmed by immunoblotting. All the mAbs reacted with SEB and with a second band which had a different electrophoretic mobility and probably represents an aggregate of SEB or SEB bound to membranes. Three mAbs reacted only with SEB and the rest showed cross-reactions with SEC1. No reactions were observed against any other serovar (SEA, SED and SEE) or other proteins.
authors.affiliation: Departamento de Patología Animal I, Facultad de Veterinaria, Universidad Complutense, Madrid, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Goyache
authors.forename: J A
authors.initials: JA
authors.lastname: Orden
authors.forename: J L
authors.initials: JL
authors.lastname: Blanco
authors.forename: J
authors.initials: J
authors.lastname: Hernández
authors.forename: A
authors.initials: A
authors.lastname: Doménech
authors.forename: G
authors.initials: G
authors.lastname: Suárez
authors.forename: E
authors.initials: E
authors.lastname: Gómez-Lucía
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology* / Q000302:isolation & purification; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000302:isolation & purification; D000918:Antibody Specificity; D000942:Antigens, Bacterial / Q000276:immunology; D015153:Blotting, Western; D003429:Cross Reactions; D004768:Enterotoxins / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006825:Hybridomas / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology / Q000302:isolation & purification; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology; D013211:Staphylococcus aureus / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D004768:Enterotoxins; D007074:Immunoglobulin G; C031818:enterotoxin B, staphylococcal