Inter-species variation of schistosome 28-kDa glutathione S-transferases.
The 28-kDa glutathione S-transferase from Schistosoma mansoni (Sm28GST) is a candidate vaccine antigen. To evaluate the antigenic and phylogenetic variations between the 28-kDa GSTs from 4 species of schistosome, we have cloned and sequenced the 28-kDa GSTs from Schistosoma haematobium (Sh28GST) and Schistosoma bovis (Sb28GST). Sb28GST and Sh28GST are more similar to each other (97%) than to Sm28GST (90%) and particularly to the 28-kDa GST from Schistosoma japonicum (Sj28GST, 77%). Antisera directed against the major Sm28GST epitopes revealed differences in the recognition of the 28-kDa GSTs from the other schistosome species suggesting that these regions have been subjected to evolutionary pressure. The consequences of such species-specific epitopes on the development of a multi-species anti-schistosome vaccine are discussed.
Trottein
F
F
Centre d'Immunologie des Maladies Transmissibles et Allergiques, Unité mixte INSERM 167-CNRS 624, Institut Pasteur, Lille, France.
Godin
C
C
Pierce
R J
RJ
Sellin
B
B
Taylor
M G
MG
Gorillot
I
I
Silva
M S
MS
Lecocq
J P
JP
Capron
A
A
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D014644:Genetic Variation / Q000235:genetics / Q000276:immunology; D005982:Glutathione Transferase / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D006801:Humans; D008969:Molecular Sequence Data; D012547:Schistosoma / Q000201:enzymology* / Q000235:genetics / Q000276:immunology; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D014612:Vaccines / Q000737:chemistry / Q000235:genetics / Q000276:immunology
D014612:Vaccines; D005982:Glutathione Transferase