[Clinical significance of direct immunobead test to detect anti-sperm antibody].
Direct immunobead test (IBT) (IgG.IgA) was carried out for 290 infertile men to detect antisperm antibodies. The results, compared with semen data analyzed by traditional methods and a computer assisted semen analyzer and with hormone levels, were as follows. 1) In 21(7.2%) out of the 290 men, IgG-immunobead (IB) binding was observed in more than 50% of their motile spermatozoa; in 10(3.4%) of the 290, IgA-IB binding. 2) In all of the cases with proven fertility, the IB binding rate was less than 60% for IgG-IB, and less than 40% for IgA-IB. This suggested that antibodies attached to sperm could be among the factors in male infertility. 3) Antibodies attached to the sperm head with binding rate of less than 40% were not considered to be a causative factor in male infertility. 4) The incidence of positive IgG-IB was higher, and also in each case the IgG-IB binding rate was higher than the IgA-IB. 5) IgG-IB binding parts of each spermatozoon included IgA-IB binding parts. 6) No significant relationship was observed between the results of traditional semen analysis, LH.FSH.PRL levels or PENETRAK and the rates of IB binding.
Takahashi
K
K
Department of Obstetrics and Gynecology, Toranomon Hospital, Tokyo.
Ogawa
K
K
Tobai
H
H
Kojima
T
T
Shioda
K
K
Izuta
M
M
Sato
K
K
D000328:Adult; D000372:Agglutination Tests / Q000379:methods; D000906:Antibodies / Q000032:analysis*; D005640:Follicle Stimulating Hormone / Q000097:blood; D006801:Humans; D007070:Immunoglobulin A / Q000032:analysis; D007074:Immunoglobulin G / Q000032:analysis; D007248:Infertility, Male / Q000276:immunology; D007986:Luteinizing Hormone / Q000097:blood; D008297:Male; D011388:Prolactin / Q000097:blood; D013081:Sperm Motility / Q000502:physiology; D013094:Spermatozoa / Q000276:immunology* / Q000502:physiology
D000906:Antibodies; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D011388:Prolactin; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone