Proacrosin binding protein: immunocomparative studies in boar, bovine, hamster, human and ram.
The cross reactivities of acidic extracts from boar, bovine, hamster, human and ram spermatozoa to a polyclonal antibody of the boar proacrosin binding protein has been investigated. The pH 3.0 extracts of the washed spermatozoa from each species were subjected to Western blot analysis using a polyclonal antibody developed against the 28 kDa boar proacrosin binding protein. The boar sperm extracts had a major 28 kD and a minor 29 kDa proacrosin binding protein. A similar protein of 29 kDa was present in the bovine and ram samples, while the hamster had bands at 22 and 25 kDa. Extracts of human sperm yielded a diffuse but distinct area of cross reactivity of about 25-30 kDa. These results demonstrate that proacrosin binding proteins are present in sperm of different mammalian species and they have similar molecular weights.
Yi
L S
LS
Department of Obstetrics and Gynecology, Washington University School of Medicine, St. Louis, Missouri 63110.
Polakoski
K L
KL
D000818:Animals; D000906:Antibodies / Q000276:immunology; D002352:Carrier Proteins / Q000276:immunology* / Q000302:isolation & purification; D002417:Cattle / Q000276:immunology*; D006224:Cricetinae / Q000276:immunology*; D006801:Humans; D008297:Male; D008970:Molecular Weight; D010802:Phylogeny; D012756:Sheep / Q000276:immunology*; D013045:Species Specificity; D013094:Spermatozoa / Q000737:chemistry* / Q000276:immunology; D013552:Swine / Q000276:immunology*
D000906:Antibodies; D002352:Carrier Proteins