In vitro evaluation of biocompatibility of surface-modified poly(methyl methacrylate) plate with rabbit lens epithelial cells.
Collagen type I was immobilized onto a poly(methyl methacrylate) (PMMA) plate by covalent bonding following surface modification by two methods. One method introduced amino groups by aminolysis with N-lithioethylenediamine (PMMA-NH2) and the other introduced carboxyl groups by graft copolymerization of acrylic acid (AAc) and acrylamide (AAm) (PMMA-COOH). Lens epithelial rabbit cells were cultured on the PMMA plate which was immobilized with collagen. Polygonal cells with a mosaic appearance were observed on the PMMA-COOH plate immobilized with collagen type I, whereas pleomorphic cells were present on the virgin PMMA and on the PMMA-NH2 plate immobilized with collagen type I. We concluded the PMMA-COOH plate immobilized with collagen type I provided a more comfortable atmosphere for lens epithelial cells, causing no metaplasia, than the other plates used in this cell culture model experiment.
Ichijima
H
H
Research Center for Biomedical Engineering, Kyoto University, Japan.
Kobayashi
H
H
Ikada
Y
Y
D000818:Animals; D002448:Cell Adhesion; D002478:Cells, Cultured; D003094:Collagen / Q000737:chemistry / Q000502:physiology*; D004847:Epithelial Cells; D007908:Lens, Crystalline / Q000166:cytology*; D008297:Male; D008422:Materials Testing; D020366:Methylmethacrylate; D008768:Methylmethacrylates / Q000737:chemistry; D008858:Microscopy, Phase-Contrast; D011817:Rabbits; D013499:Surface Properties
D008768:Methylmethacrylates; D020366:Methylmethacrylate; D003094:Collagen