Effect of fermented oatmeal soup on the cholesterol level and the Lactobacillus colonization of rat intestinal mucosa.
Rats were fed with freeze-dried oatmeal soup fermented by six different Lactobacillus strains from rat and man; the formula is intended for enteral feeding. The serum cholesterol levels after 10 d were lower for rats eating oatmeal as compared to a commercial product, Biosorb Sond. Colonizing ability of the administered strains were evaluated in vivo. Only Lactobacillus reuteri R21c were able to, effectively, colonizing the mucosa; it represented about 30% of the Lactobacillus population 24 d after termination of the administration. L. reuteri R21c was easily recognized by the ability to produce a yellow pigment on agar plates. The identity was confirmed by carbohydrate fermentations (API 50CH), plasmid pattern and endonuclease restriction analysis of the chromosomal DNA.
Molin
G
G
Food- and Environmental Microbiology, Chemical Center, Lund, Sweden.
Andersson
R
R
Ahrné
S
S
Lönner
C
C
Marklinder
I
I
Johansson
M L
ML
Jeppsson
B
B
Bengmark
S
S
D000818:Animals; D002784:Cholesterol / Q000097:blood*; D004269:DNA, Bacterial / Q000032:analysis; D004040:Dietary Carbohydrates / Q000008:administration & dosage*; D002523:Edible Grain; D005285:Fermentation; D007413:Intestinal Mucosa / Q000382:microbiology*; D007778:Lactobacillus / Q000254:growth & development*; D008297:Male; D010957:Plasmids / Q000235:genetics; D051381:Rats; D011919:Rats, Inbred Strains
D004269:DNA, Bacterial; D004040:Dietary Carbohydrates; D002784:Cholesterol