title: Peptidyl-prolyl cis-trans isomerase from Bacillus subtilis. A prokaryotic enzyme that is highly sensitive to cyclosporin A.
abstract: Cyclophylins are members of a class of proteins with peptidyl-prolyl cis-trans isomerase activity. These enzymes bind the immunosuppressive agent, cyclosporin A (CsA), which acts as a competitive inhibitor. The peptidyl-prolyl cis-trans isomerase from Bacillus subtilis (PPIase) was purified to homogeneity in a 4-step purification procedure, which resulted in a 100-fold protein purification with a yield of 5%. Coomassie blue-stained SDS-PAGE revealed a single band of about 18 kDa. PPIase activity was determined using synthetic peptides as substrates in a 2-step reaction coupled to chymotrypsin. Treatment of Bacillus subtilis PPIase by CsA revealed an inhibition constant of Ki = 175 nM, which differs from cyclophilin of enterobacteria such as E. coli or Salmonella typhimurium and is in the range of human enzymes.
authors.affiliation: Philips-Universität Marburg, Fachbereich Chemie/Biochemie, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Herrler
authors.forename: H
authors.initials: H
authors.lastname: Bang
authors.forename: K
authors.initials: K
authors.lastname: Brune
authors.forename: G
authors.initials: G
authors.lastname: Fischer
authors.forename: M A
authors.initials: MA
authors.lastname: Marahiel
mesh_terms: D000591:Amino Acid Isomerases / Q000187:drug effects / Q000302:isolation & purification / Q000378:metabolism*; D001412:Bacillus subtilis / Q000187:drug effects / Q000201:enzymology*; D002352:Carrier Proteins / Q000187:drug effects / Q000302:isolation & purification / Q000378:metabolism*; D002852:Chromatography, Ion Exchange; D016572:Cyclosporine / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel; D007166:Immunosuppressive Agents / Q000494:pharmacology; D019696:Peptidylprolyl Isomerase
chemical_list: D002352:Carrier Proteins; D007166:Immunosuppressive Agents; D016572:Cyclosporine; D000591:Amino Acid Isomerases; D019696:Peptidylprolyl Isomerase