title: Differences in the onset of the inflammatory response to cutaneous leishmaniasis in resistant and susceptible mice.
abstract: Sites of cutaneous infection with Leishmania major in genetically susceptible (BALB/c) and resistant (C57B1/6) mice were investigated for the early inflammatory response (6 h to 12 days) by electron microscopy combined with enzyme-histochemical methods. Susceptible BALB/c mice spontaneously recruited only polymorphonuclear leukocytes (PMNs) at the site of infection. Infiltrating mononuclear phagocytes (and eosinophils) were first observed at day 1 in a ratio equal to the influx of PMNs (about 40%). This pattern persisted during the following 11 days of infection. In the resistant C57/B16 mice, the first cellular infiltrate at the infected site contained mononuclear phagocytes (25%) and eosinophils (15%) besides PMNs (60%). Within 3 days after infection, mononuclear phagocytes became the dominant population of cells in cutaneous lesions (up to 80%). It was found in situ that L. major accumulated and replicated in immature macrophages, that is, intermediate stages between monocytes and resident macrophages, which were found in lesions of both strains. The burden of parasites was, however, degraded more rapidly by the infiltrating cells of the resistant mice than by those of the susceptible ones. Within the first 4 days of infection, the parasites were found in PMNs, mononuclear phagocytes, and extracellular spaces in both strains. In susceptible mice this distribution pattern persisted up to 12 days after infection; in resistant C57B1/6 mice parasites accumulated inside mononuclear phagocytes within this period. It is concluded that the features of acute inflammation during leishmaniasis in BALB/c mice are sustained over a prolonged period that is ineffective in the elimination of L. major.
authors.affiliation: Institute of Experimental Dermatology, University of Münster, Germany.
authors.forename: W J
authors.initials: WJ
authors.lastname: Beil
authors.forename: G
authors.initials: G
authors.lastname: Meinardus-Hager
authors.forename: D C
authors.initials: DC
authors.lastname: Neugebauer
authors.forename: C
authors.initials: C
authors.lastname: Sorg
mesh_terms: D000818:Animals; D007249:Inflammation / Q000473:pathology*; D016773:Leishmaniasis, Cutaneous / Q000276:immunology* / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009504:Neutrophils / Q000473:pathology; D010586:Phagocytes / Q000473:pathology; D013045:Species Specificity