Characterization of a glial associated antigen GA-1 by monoclonal antibody.
A glial antigen (GA-1) was identified by monoclonal antibodies (MAb) raised against C6 rat glioma cells. MAb-7D3 (IgG2a kappa) revealed GA-1 as a single protein band with a Rf value of 0.09 by the use of basic-PAGE Western blot. SDS-PAGE Western blot and radioimmunoprecipitation (RIP) further resolved GA-1 into two subunits with a molecular weight of 200 and 78 kDa respectively. Subcellular localization by immunocytochemical staining revealed its cytosolic presence with a punctate pattern perinuclearly. Significant expression of GA-1 may be detected in 4 glioma or glial cell lines derived from rat brain. However, no expression may be detected in the rest of the 18 mammalian cell lines or primary neural cell cultures examined. All of the above data thereby suggest that GA-1 may be glial specific whereas the epitope of GA-1 defined by MAb-7D3 is species (rat) specific.
Chang
A C
AC
Institute of Neuroscience, National Yang-Ming Medical College, Taipei, Taiwan ROC.
Lin
Y H
YH
Chou
R L
RL
Liao
G Y
GY
Chang
N C
NC
D000818:Animals; D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification*; D015153:Blotting, Western; D001932:Brain Neoplasms / Q000276:immunology*; D002460:Cell Line; D005910:Glioma / Q000276:immunology*; D006801:Humans; D007150:Immunohistochemistry; D051379:Mice; D009457:Neuroglia / Q000276:immunology*; D011817:Rabbits; D051381:Rats; D013045:Species Specificity; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm