The screening for common drugs of abuse in whole blood by means of EMIT-ETS and FPIA-ADx urine immunoassays.
The purpose of the paper was to compare the performance of ETS (EMIT) and ADx (FPIA) analyzers for screening blood samples for drugs of abuse after 2 alternative pretreatment procedures (acetone precipitation and ultrafiltration). Cannabinoids, benzodiazepines and benzoylecgonine were not detectable with both assays after ultrafiltration. The detectability of morphine in blood ultrafiltrates was distinctly lower than after acetone precipitation. The comparison of results obtained with ETS and ADx after acetone precipitation showed that ETS assay is slightly more sensitive but ADx is slightly more reproducible. Both assays may be used for blood examination with similar cut-off values. The ETS analyzer gave much better analytical performance (speed, flexibility) and lower reagent costs than the ADx analyzer.
Maier
R D
RD
Institute of Forensic Medicine, Technical University RWTH, Aachen, Federal Republic of Germany.
Erkens
M
M
Hoenen
H
H
Bogusz
M
M
D000096:Acetone; D011232:Chemical Precipitation; D005069:Evaluation Studies as Topic; D016231:Fluorescence Polarization Immunoassay / Q000295:instrumentation / Q000379:methods / Q000592:standards*; D005554:Forensic Medicine / Q000379:methods; D006801:Humans; D007124:Immunoenzyme Techniques / Q000295:instrumentation / Q000592:standards*; D011180:Postmortem Changes; D012680:Sensitivity and Specificity; D015813:Substance Abuse Detection / Q000295:instrumentation / Q000379:methods / Q000592:standards*; D014462:Ultrafiltration
D000096:Acetone