title: Periplasmic location of nitrous oxide reductase and its apoform in denitrifying Pseudomonas stutzeri.
abstract: Immunogold labelling techniques on ultrathin sections of low temperature embedded cells yielded evidence for the periplasmic location of the respiratory enzymes N2O reductase and nitrite reductase (cytochrome cd1) in Pseudomonas stutzeri strain ZoBell. Cell fractionation by spheroplast preparation and two-dimensional electrophoresis showed the absence of a membrane association of these enzymes. Immunocytochemical localization of N2O reductase in a mutant strain deficient in the chromophore of N2O reductase showed the gold label at the cell periphery, indicating that the copper chromophore processing takes place after export of this protein's apoform.
authors.affiliation: Lehrstuhl für Mikrobiologie, Universität Karlsruhe, Federal Republic of Germany.
authors.forename: H
authors.initials: H
authors.lastname: Körner
authors.forename: F
authors.initials: F
authors.lastname: Mayer
mesh_terms: D001426:Bacterial Proteins / Q000302:isolation & purification; D003593:Cytoplasm / Q000201:enzymology* / Q000648:ultrastructure; D015180:Electrophoresis, Gel, Two-Dimensional; D007150:Immunohistochemistry; D016253:Microscopy, Immunoelectron; D009572:Nitrite Reductases / Q000302:isolation & purification*; D010088:Oxidoreductases / Q000302:isolation & purification*; D011549:Pseudomonas / Q000201:enzymology* / Q000648:ultrastructure; D013104:Spheroplasts; D013347:Subcellular Fractions / Q000201:enzymology
chemical_list: D001426:Bacterial Proteins; D010088:Oxidoreductases; D009572:Nitrite Reductases; C022763:nitrous oxide reductase