Investigation of spatial relationships and energy transfer between complexes B800-850 and B890-RC from Chromatium minutissimum reconstituted into liposomes.
Spatial relationships between different pigment-protein complexes in the membranes of the purple photosynthetic bacterium, Chromatium minutissimum, have been studied. The possibility of restoring the function of efficient excitation energy transfer from bacteriochlorophyll molecules to the reaction centers in the system of soybean liposomes, reconstituted with pigment-protein complexes B800-850 and B890-RC from C. minutissimum, has been explored. The chemical cross-linking method, together with stationary and picosecond spectrally resolved fluorescence measurements were employed. It has been shown that after the incorporation of the complexes into the liposome membranes conditions for directed excitation energy transfer from the light-harvesting pigments to the reaction centers are created, which are less optimal, however, than those in the native state. Possible reasons are considered.
Moskalenko
A A
AA
Institute of Soil Sciences and Photosynthesis, Russian Academy of Sciences, Puschino.
Toropygina
O
O
Godik
V I
VI
Timpmann
K
K
Freiberg
A
A
D001426:Bacterial Proteins / Q000378:metabolism; D002844:Chromatium / Q000378:metabolism*; D003432:Cross-Linking Reagents; D004591:Electrophoresis, Polyacrylamide Gel; D008081:Liposomes; D010860:Pigments, Biological / Q000378:metabolism; D013050:Spectrometry, Fluorescence
D001426:Bacterial Proteins; D003432:Cross-Linking Reagents; D008081:Liposomes; D010860:Pigments, Biological