[Microheterogeneity of serum glycoproteins in alcoholic liver disease].
It has been reported that microheterogeneity (M-HT) of serum glycoproteins including transferrin is found in alcoholic liver disease (ALD). In the present study, M-HT of serum glycoproteins in ALD patients was analyzed using the Western blotting technique after isoelectric focusing. M-HT was found in serum alpha 1-antitrypsin, alpha 2-macroglobulin, ceruloplasmin, alpha 1-acid glycoprotein and hemopexin as well as transferrin, but not in serum prealbumin. M-HT disappeared following treatment with sialidase in one group of glycoproteins, but not in another group of glycoproteins. In hemopexin, M-HT was recognized only after treatment with sialidase. These results suggest that mechanisms of the appearance of M-HT of serum glycoproteins in ALD may differ. One mechanism is the interference of glycosylation of glycoproteins in the Golgi apparatus, and another is the decrease of asialo-protein receptors in hepatocytes.
Wang
J S
JS
Department of Internal Medicine, Kanazawa Medical University.
Tsutsumi
M
M
Takada
A
A
D015153:Blotting, Western; D006023:Glycoproteins / Q000097:blood*; D006801:Humans; D007525:Isoelectric Focusing; D008108:Liver Diseases, Alcoholic / Q000097:blood*
D006023:Glycoproteins