Purification and characterization of two forms of soluble NADH cytochrome b5 reductases from human erythrocytes.
1. Two forms of soluble NADH cytochrome b5 reductase were purified from human erythrocytes. Two distinct fractions both having the NADH cytochrome b5 reductase activity eluted from the second DEAE-cellulose column were further purified by ultrafiltration and 5'-ADP-agarose affinity chromatography. 2. The final preparations were purified 9070- and 4808-fold, respectively, over hemolysate. Both reductases exhibited identical electrophoretic patterns when subjected to SDS-PAGE and apparent monomer Mr of each reductase was determined to be 32,000 +/- 1300. 3. Vmax values of reductase II for the various electron acceptors, namely, 2,6-dichlorophenolindophenol, ferricyanide and cytochrome c through cytochrome b5 were found to be 1.9, 1.8 and 2 times higher than those of reductase I. 4. Some differences were noted for reductase I and reductase II fractions. Their elution profiles from a second DEAE-cellulose column were quite different and that suggested that reductase II is more acidic than reductase I. Reductase II was found to be more sensitive to heat treatment than reductase I.
Arinç
E
E
Department of Biology, Middle East Technical University, Ankara, Turkey.
Güray
T
T
Saplakoğlu
U
U
Adali
O
O
D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D003579:Cytochrome Reductases / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D042966:Cytochrome-B(5) Reductase; D004795:Enzyme Stability; D004912:Erythrocytes / Q000201:enzymology*; D006358:Hot Temperature; D006801:Humans; D007700:Kinetics; D012995:Solubility
D003579:Cytochrome Reductases; D042966:Cytochrome-B(5) Reductase