Utilization of methoxylated benzoates and formation of intermediates by Desulfotomaculum thermobenzoicum in the presence or absence of sulfate.
Desulfotomaculum thermobenzoicum strain TSB (DSM 6193) was found to utilize some methoxylated benzoates as carbon and energy source with or without sulfate. 3- or 4-Methoxybenzoate, vanillate (4-hydroxy-3-methoxybenzoate), syringate (3,5-dimethoxy-4-hydroxybenzoate) and 3,4,5-trimethoxybenzoate were converted to corresponding hydroxybenzoates. However, neither 2-methoxybenzoate nor 2,6-dimethoxybenzoate was utilized. The organism grew acetogenically on each of the methoxylated benzoates in the absence of sulfate. 3,4-Dihydroxy-5-methoxybenzoate was detected during conversion of syringate, and syringate and 3,4-dihydroxy-5-methoxybenzoate were detected during conversion of 3,4,5-trimethoxybenzoate as intermediates. These findings indicate that 4-methoxyl-group is most readily cleaved, whereas 2-methoxyl-group is not utilized by the organism.
Tasaki
M
M
Institute of Technology, Shimizu Corporation, Tokyo, Japan.
Kamagata
Y
Y
Nakamura
K
K
Mikami
E
E
D000085:Acetates / Q000378:metabolism; D019342:Acetic Acid; D000445:Aldehyde Oxidoreductases / Q000032:analysis; D001406:Bacillaceae / Q000378:metabolism*; D001565:Benzoates / Q000378:metabolism*; D005707:Gallic Acid / Q000031:analogs & derivatives / Q000378:metabolism; D062425:Hydroxybenzoate Ethers; D062385:Hydroxybenzoates / Q000378:metabolism; D009097:Multienzyme Complexes; D014641:Vanillic Acid / Q000031:analogs & derivatives / Q000378:metabolism
D000085:Acetates; D001565:Benzoates; D062425:Hydroxybenzoate Ethers; D062385:Hydroxybenzoates; D009097:Multienzyme Complexes; C004520:4-anisic acid; D005707:Gallic Acid; C056267:3-methoxybenzoic acid; C001945:syringic acid; D000445:Aldehyde Oxidoreductases; C019823:carbon monoxide dehydrogenase; D014641:Vanillic Acid; D019342:Acetic Acid; C005854:3,4,5-trimethoxybenzoic acid