title: Purification of an enterotoxin produced by Bacillus cereus by immunoaffinity chromatography using a monoclonal antibody.
abstract: A murine monoclonal antibody (MAb) with high reactivity to an enterotoxin produced by Bacillus cereus was used to prepare an immunoadsorbent for purification of the enterotoxin. By immunoaffinity chromatography using the immunoadsorbent, approximately 25% of crude enterotoxin applied was recovered in the eluate. The purified enterotoxin was found to be electrophoretically and antigenically homogeneous. It also showed vascular permeability activity and mouse lethality, and caused fluid accumulation in mouse ligated intestinal loops, whereas it did not show any hemolytic and lecithinase activities. Thus, immunoaffinity chromatography proved useful in the purification of enterotoxin produced by B. cereus in terms of recovery, purity, and relative ease of performing the purification.
authors.affiliation: Department of Veterinary Medicine, Faculty of Agriculture, Iwate University, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Shinagawa
authors.forename: T
authors.initials: T
authors.lastname: Takechi
authors.forename: N
authors.initials: N
authors.lastname: Matsusaka
authors.forename: S
authors.initials: S
authors.lastname: Sugii
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D001409:Bacillus cereus / Q000737:chemistry*; D002846:Chromatography, Affinity; D004768:Enterotoxins / Q000302:isolation & purification* / Q000633:toxicity; D051379:Mice
chemical_list: D000911:Antibodies, Monoclonal; D004768:Enterotoxins