Membrane permeable fluorogenic rhodamine substrates for selective determination of cathepsin L.
The dipeptidyl rhodamine diamide substrates (Z-Phe-Arg)2-R110 and (Z-Arg-Arg)2-R110 are 820- and 360-fold more selective for cathepsin L than for cathepsin B allowing a sensitive determination of cathepsin L activity in the presence of high activity of cathepsin B. The results obtained with cell lysates suggest that the cysteine proteinase activity of vital macrophages detected by flow cytometry with these substrates is mainly due to cathepsin L.
Assfalg-Machleidt
I
I
Institut für Physiologische Chemie, Physikalische Biochemie und Zellbiologie, Universität München.
Rothe
G
G
Klingel
S
S
Banati
R
R
Mangel
W F
WF
Valet
G
G
Machleidt
W
W
D000818:Animals; D002401:Cathepsin B / Q000032:analysis; D056668:Cathepsin L; D002403:Cathepsins / Q000032:analysis*; D003374:Coumarins / Q000378:metabolism; D003546:Cysteine Endopeptidases; D010450:Endopeptidases; D005434:Flow Cytometry; D005456:Fluorescent Dyes; D006801:Humans; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007700:Kinetics; D009000:Monocytes / Q000201:enzymology; D009504:Neutrophils / Q000201:enzymology; D010539:Permeability; D010587:Phagocytosis; D051381:Rats; D011919:Rats, Inbred Strains; D012235:Rhodamines / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013379:Substrate Specificity
D003374:Coumarins; D005456:Fluorescent Dyes; D012235:Rhodamines; D002403:Cathepsins; D010450:Endopeptidases; D003546:Cysteine Endopeptidases; D002401:Cathepsin B; C534281:CTSL protein, human; D056668:Cathepsin L; C534280:Ctsl protein, rat