The study of properties of immunoreactive estradiol secreted by adrenals and ovaries of immature female rats.
Some properties of immunoreactive estradiol secreted by adrenals and ovaries of immature female rats were studied. It was shown that adrenals and ovaries of 15-day-old animals secrete approximately equal amounts of immunoreactive estradiol, with thin-layer chromatogram of immunoreactive material being identical for both glands. Adrenal- as well as ovarian-derived estradiol can bind to uterine cytosol estradiol receptors with the formation of complexes, which can be activated in the cell-free system. The removal of either adrenals or ovaries causes a decrease of cytosol estradiol receptor levels in the uterus 6 hours after the operation. From these results and previously reported data, nothing suggests that immunoreactive estradiol secreted by the adrenals of immature rats should be regarded as "spurious" hormone.
Tinnikov
A A
AA
Department of Physiological Genetics, Institute of Cytology and Genetics, Novosibirsk, Russia.
Bazhan
N M
NM
Yakovleva
T V
TV
D000311:Adrenal Glands / Q000378:metabolism*; D000818:Animals; D002855:Chromatography, Thin Layer; D003600:Cytosol / Q000737:chemistry / Q000378:metabolism; D004958:Estradiol / Q000737:chemistry / Q000378:metabolism*; D005260:Female; D010053:Ovary / Q000378:metabolism*; D011869:Radioligand Assay; D051381:Rats; D011919:Rats, Inbred Strains; D011959:Receptors, Estradiol / Q000378:metabolism*; D014599:Uterus / Q000378:metabolism
D011959:Receptors, Estradiol; D004958:Estradiol