Cytotoxicity of cyanobacterium Microcystis aeruginosa.
Cytotoxic effects of crude extracts and fractions of the purification steps towards Microcystin-LR (MCYST-LR) were investigated in vitro. Cytotoxicity was evaluated by measure of lactate dehydrogenase liberation of Chang liver cells and by hemolysis. Crude extracts of strain PCC 7806 damaged the cells within a few minutes. In contrast, MCYST-LR did not show any detectable cytotoxic effects. The cytotoxic activity could be related to a heat-labile substance with a molecular weight of about 30,000 Da.
Henning
K
K
Institute of Microbiology, Federal Armed Forces, Medical Academy, Munich.
Cremer
J
J
Meyer
H
H
D000818:Animals; D001427:Bacterial Toxins / Q000302:isolation & purification / Q000633:toxicity*; D002460:Cell Line; D003603:Cytotoxins / Q000302:isolation & purification / Q000633:toxicity*; D008387:Marine Toxins; D052998:Microcystins; D046931:Microcystis / Q000502:physiology*; D010456:Peptides, Cyclic / Q000302:isolation & purification / Q000633:toxicity*
D001427:Bacterial Toxins; D003603:Cytotoxins; D008387:Marine Toxins; D052998:Microcystins; D010456:Peptides, Cyclic; C057862:cyanoginosin LR