Determination of diclazuril in animal feed by liquid chromatography.
A method is described for the determination of diclazuril (Janssen Research Compound R64433; trademark Clinacox) in chicken feed at the mg kg(-1) level. Compound R062646, a structure analogous to diclazuril, was used as the interna standard. The drug was extracted from food with acidified methanol. Diclazuril was then isolated by means of solid-phase extraction with a cartridge containing a C18 phase. The eluate was evaporated and the residue redissolved in dimethylformamide. An aliquot was injected onto a reversed-phase high-performance liquid chromatographic column and the drug substance quantified at 280 nm by an ultraviolet detector. Extraction (absolute) recoveries of 85% for both internal standard and diclazuril were obtained. The method is suitable for diclazuril concentrations ranging from 0.1 to 1.5 mg kg(-1). Method validation data are presented.
De Kock
J
J
Analytical Research Department, Janssen Research Foundation, Beerse, Belgium.
De Smet
M
M
Sneyers
R
R
D000821:Animal Feed / Q000032:analysis*; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D003049:Coccidiostats / Q000032:analysis*; D009570:Nitriles / Q000032:analysis*; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet; D014227:Triazines / Q000032:analysis*
D003049:Coccidiostats; D009570:Nitriles; D014227:Triazines; C057884:diclazuril