Nucleotide sequence analysis of 95 kb near the 3' end of the murine T-cell receptor alpha/delta chain locus: strategy and methodology.
The nucleotide sequence of a region at the 3' terminus of the murine T-cell receptor alpha/delta chain locus is presented. This region, which encodes the constant region genes for alpha and delta chain polypeptides and all 50 joining gene segments for the alpha chain polypeptide, spans 94,647 bp and includes more than 50 noncoding sequence elements important for T-cell receptor gene rearrangement and expression. DNA sequencing of this region included complete analysis of two cosmid clones and five additional restriction fragments using a random subcloning approach with various manual and automated sequencing strategies. The automated sequencing strategies hold considerable promise for future large-scale DNA sequencing efforts.
Wilson
R K
RK
Division of Biology, California Institute of Technology, Pasadena 91125.
Koop
B F
BF
Chen
C
C
Halloran
N
N
Sciammis
R
R
Hood
L
L
D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D003360:Cosmids; D004247:DNA; D051379:Mice; D008969:Molecular Sequence Data; D010957:Plasmids; D011948:Receptors, Antigen, T-Cell / Q000235:genetics*; D012045:Regulatory Sequences, Nucleic Acid
D011948:Receptors, Antigen, T-Cell; D004247:DNA