The effect of EDTA and serum on endogenous porphyrin accumulation and photodynamic sensitization of human K562 leukemic cells.
The interrelationship between the effect of serum on the induction of porphyrin synthesis, intracellular porphyrin accumulation and photodynamic sensitization of human K562 cells is described. Endogenous porphyrins, synthesized from supplemented 5-amino levulinic acid (5-ALA), were shown to accumulate in the cells, while an addition of serum triggered porphyrin translocation from the cell to the serum. In order to enhance porphyrin accumulation in the cells themselves, they were further stimulated by EDTA, which in combination with 5-ALA reduces Fe++ cellular content. The higher porphyrin cellular content under EDTA and 5-ALA induction was exploited to photoinactivate the human leukemic cells by more then 3 orders of magnitude.
Hanania
J
J
Life Science Department, Bar-Ilan University, Ramat-Gan, Israel.
Malik
Z
Z
D000622:Aminolevulinic Acid / Q000494:pharmacology*; D001769:Blood; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D003470:Culture Media; D004492:Edetic Acid / Q000494:pharmacology*; D006801:Humans; D007700:Kinetics; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive; D008027:Light; D011166:Porphyrins / Q000096:biosynthesis / Q000378:metabolism*; D014466:Ultraviolet Rays
D003470:Culture Media; D011166:Porphyrins; D000622:Aminolevulinic Acid; D004492:Edetic Acid