title: [Analysis of human growth hormone-binding protein in plasma by high pressure liquid chromatography].
abstract: A technique using high pressure liquid chromatography gel filtration was used to evaluate GH-binding proteins (GH-BPs) in human plasma; eluate was monitored for radioactivity in a gamma-detection system connected to a computer. Plasma (200 microliters) was incubated with 125I-human (h) GH (200,000 cpm) at 4 degrees C for 20 hours. Our GH binding assay offers important gains in terms of rapidity and resolution; it has permitted a clear separation and characterization of the two GH-binding components present in human plasma.
authors.affiliation: INSERM U.30, Hôpital des Enfants-Malades, Paris.
authors.forename: A
authors.initials: A
authors.lastname: Tar
authors.forename: J F
authors.initials: JF
authors.lastname: Hocquette
authors.forename: J C
authors.initials: JC
authors.lastname: Souberbielle
authors.forename: J P
authors.initials: JP
authors.lastname: Clot
authors.forename: R
authors.initials: R
authors.lastname: Brauner
authors.forename: M C
authors.initials: MC
authors.lastname: Postel-Vinay
mesh_terms: D002352:Carrier Proteins / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D013006:Growth Hormone / Q000097:blood / Q000378:metabolism*; D006801:Humans; D011485:Protein Binding
chemical_list: D002352:Carrier Proteins; D013006:Growth Hormone; C051815:somatotropin-binding protein
vernacular_title: A humán növekedési hormon kötöfehérjék vizsgálata plazmában magas nyomású folyadékkromatográfiával.