title: Experimental reproduction of ELEM. A study to determine the minimum toxic dose in ponies.
abstract: An experiment to gain insight into the minimum toxic dose of fumonisins was conducted by feeding ponies rations with known fumonisin concentrations. Naturally contaminated corn screenings (CS) were blended with pellets, corn, and molasses to formulate individual daily diets. One group of 4 ponies was fed a ration with fumonisin B1 (FB1) varying from less than 1 ppm to 22 ppm. A second group of 5 ponies was fed a ration at varying rates containing 8 ppm FB1 for 180 days. A panel of clinical chemistry parameters was evaluated twice weekly for both groups. One pony in the first group died of equine leukoencephalomalacia (ELEM) after 225 days of which the final 55 days' diet contained 22 ppm FB1. Approximately 9 days prior to death, this animal experienced elevated liver chemistry values. All 5 ponies in the second group experienced mild, transient, clinical signs; were euthanized at 180 days; and had mild, histopathological brain lesions.
authors.affiliation: Animal and Plant Health Inspection Service, National Veterinary Services Laboratories, USDA, Ames, IA 50010.
authors.forename: T M
authors.initials: TM
authors.lastname: Wilson
authors.forename: P F
authors.initials: PF
authors.lastname: Ross
authors.forename: D L
authors.initials: DL
authors.lastname: Owens
authors.forename: L G
authors.initials: LG
authors.lastname: Rice
authors.forename: S A
authors.initials: SA
authors.lastname: Green
authors.forename: S J
authors.initials: SJ
authors.lastname: Jenkins
authors.forename: H A
authors.initials: HA
authors.lastname: Nelson
mesh_terms: D000821:Animal Feed / Q000032:analysis / Q000633:toxicity*; D000818:Animals; D001921:Brain / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004678:Encephalomalacia / Q000139:chemically induced / Q000662:veterinary*; D005260:Female; D037341:Fumonisins; D006734:Horse Diseases / Q000139:chemically induced*; D006736:Horses; D008297:Male; D009183:Mycotoxins / Q000008:administration & dosage / Q000032:analysis / Q000633:toxicity*
chemical_list: D037341:Fumonisins; D009183:Mycotoxins; C056933:fumonisin B1