Intracerebral transplants of primary muscle cells: a potential 'platform' for transgene expression in the brain.
After the transplantation of rat primary muscle cells into the caudate or cortex of recipient rats, the muscle cells were able to persist for at least 6 months. Muscle cells transfected with expression plasmids prior to transplantation were able to express reporter genes in the brains for at least 2 months. These results suggest that muscle cells might be a useful 'platform' for transgene expression in the brain.
Jiao
S
S
Department of Pediatrics and Medical Genetics, Waisman Center, University of Wisconsin, Madison 53706.
Schultz
E
E
Wolff
J A
JA
D000818:Animals; D002421:Caudate Nucleus; D002470:Cell Survival / Q000235:genetics; D002540:Cerebral Cortex; D015870:Gene Expression / Q000235:genetics*; D009132:Muscles / Q000637:transplantation*; D010957:Plasmids / Q000235:genetics; D051381:Rats; D011917:Rats, Inbred Lew; D033581:Stem Cell Transplantation; D014162:Transfection; D016042:Transplantation, Heterotopic / Q000502:physiology*