title: A general, PCR-based method for single or combinatorial oligonucleotide-directed mutagenesis on pUC/M13 vectors.
authors.affiliation: Instituto de Biotecnología, UNAM, Cuernavaca, Morelos, Mexico.
authors.forename: E
authors.initials: E
authors.lastname: Merino
authors.forename: J
authors.initials: J
authors.lastname: Osuna
authors.forename: F
authors.initials: F
authors.lastname: Bolívar
authors.forename: X
authors.initials: X
authors.lastname: Soberón
mesh_terms: D001483:Base Sequence; D004926:Escherichia coli; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009841:Oligonucleotides / Q000235:genetics*; D010957:Plasmids; D016133:Polymerase Chain Reaction / Q000379:methods*; D014170:Transformation, Genetic
chemical_list: D009841:Oligonucleotides