[Identification of the gene for the immunodominant p35 protein from vaccinia virus].
A major immunodominant envelope protein p35 of vaccina virus was purified by means of extraction from virions with detergent NP-40. The protein was cleaved with CNBr, four homogenous peptides were isolated and their N-terminal amino acid sequences were determined. Computer search in a protein sequences data bank revealed that the immunodominant protein p35 of vaccinia virus is encoded by H3 gene in HindIII-H fragment of vaccinia virus genome.
Zinov'ev
V V
VV
Ovechkina
L G
LG
Matskova
L V
LV
Balakhnin
S M
SM
Malygin
E G
EG
Chertov
O Iu
OIu
Telezhinskaia
I N
IN
Zaĭtseva
E V
EV
Golubeva
T B
TB
D000595:Amino Acid Sequence; D015153:Blotting, Western; D002850:Chromatography, Gel; D003488:Cyanogen Bromide; D004591:Electrophoresis, Polyacrylamide Gel; D005814:Genes, Viral; D007106:Immune Sera; D016056:Immunodominant Epitopes / Q000235:genetics*; D008969:Molecular Sequence Data; D011863:Radioimmunoassay; D014616:Vaccinia virus / Q000235:genetics* / Q000276:immunology; D014759:Viral Envelope Proteins / Q000235:genetics*
D007106:Immune Sera; D016056:Immunodominant Epitopes; D014759:Viral Envelope Proteins; C072495:immunodominant protein p35, vaccinia virus; D003488:Cyanogen Bromide
Identifikatsiia gena immunodominantnogo belka p35 virusa ospovaktsiny.