title: Differential localization of G proteins, Gi and Go, in the olfactory epithelium and the main olfactory bulb of the rat.
abstract: The immunohistochemical localization of proteins Gi1 (plus Gi3). Gi2 and Go was studied in the olfactory epithelium and the main olfactory bulb of rats, using purified antibodies to the respective alpha subunits and beta gamma subunits of these G proteins. In the olfactory epithelium, only a restricted population of olfactory cells was immunopositive for Gi2 alpha, but others were not. The immunoreactivity for Gi1 alpha/Gi3 alpha was not observed. The olfactory epithelium was immunopositive for both Go alpha and beta gamma, but its apical surface was immunopositive only for beta gamma. In the main olfactory bulb, all layers were intensely immunopositive for Go alpha and beta gamma but weakly for Gi2 alpha. In contrast to the negative or weak immunostainings in the olfactory nerve fiber layer and glomeruli, the molecular and the internal granular layers were intensely immunopositive for Gi1 alpha/Gi3 alpha. These findings suggest the functional difference among Gi1/Gi3, Gi2 and Go in the signal transduction in the olfactory system.
authors.affiliation: Department of Biochemistry, Institute for Developmental Research, Aichi Prefectural Colony, Kasugai, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Shinohara
authors.forename: K
authors.initials: K
authors.lastname: Kato
authors.forename: T
authors.initials: T
authors.lastname: Asano
mesh_terms: D000818:Animals; D000918:Antibody Specificity; D004847:Epithelial Cells; D019204:GTP-Binding Proteins / Q000032:analysis*; D007150:Immunohistochemistry; D009297:Nasal Mucosa / Q000166:cytology*; D009412:Nerve Fibers / Q000648:ultrastructure; D009830:Olfactory Bulb / Q000166:cytology*; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D019204:GTP-Binding Proteins