title: Fourier transform resonance Raman spectroscopy of phytochrome.
abstract: The Pr and Pfr forms of phytochrome in H2O and D2O have been studied by Fourier transform resonance Raman spectroscopy with near-infrared excitation (1064 nm). It is demonstrated that this technique is a powerful method for analyzing the chromophore structures of photosensitive pigments. The high spectral quality allows discussion of vibrational assignments based on an empirical approach using previously published data obtained from model compounds. The reduction in intensity of a high-frequency band assigned to the ring-C/D methine bridge vibration is an indication for the non-coplanarity of the ring D in Pfr. The high intensity of a C-H out-of-plane vibration also supports this hypothesis. In Pr, a broad peak at approximately 1100 cm-1 is assigned to an out-of-plane vibration of a strongly hydrogen-bonded pyrrole C=NH+ group. It is missing in Pfr, suggesting deprotonation of the corresponding ring during the transformation from Pr to Pfr.
authors.affiliation: Max-Planck-Institut für Strahlenchemie, Mülheim a. d. Ruhr, FRG.
authors.forename: P
authors.initials: P
authors.lastname: Hildebrandt
authors.forename: A
authors.initials: A
authors.lastname: Hoffmann
authors.forename: P
authors.initials: P
authors.lastname: Lindemann
authors.forename: G
authors.initials: G
authors.lastname: Heibel
authors.forename: S E
authors.initials: SE
authors.lastname: Braslavsky
authors.forename: K
authors.initials: K
authors.lastname: Schaffner
authors.forename: B
authors.initials: B
authors.lastname: Schrader
mesh_terms: D055598:Chemical Phenomena; D002627:Chemistry, Physical; D005583:Fourier Analysis; D015394:Molecular Structure; D010777:Photochemistry; D010834:Phytochrome / Q000737:chemistry*; D010944:Plants / Q000737:chemistry*; D013059:Spectrum Analysis, Raman
chemical_list: D010834:Phytochrome