Prevention of X-ray-induced late dermal necrosis in the pig by treatment with multi-wavelength light.
Low-level light from a multi-wavelength array of light sources has been used to prevent late X-ray-induced dermal necrosis in the pig. Skin fields, measuring 4 x 4 cm on the flank, were irradiated with a single dose of 23.4 Gy of X-rays. This X-ray dose was associated with the development of a 100% incidence of dermal necrosis, 10-16 weeks after irradiation. These irradiated skin sites were subsequently exposed to light of 660, 820, 880, and 950 nm wavelengths from a gallium aluminium arsenide multiple wavelength multidiode cluster probe (Biotherapy Medical Laser, 3ML), three times a week, from 4 to 16 weeks or 6 to 16 weeks after X-irradiation. The skin fields were exposed to the light pulsating at either 2.5 Hz or 5 kHz. With light pulsating at 5 kHz, energy densities of 0.22, 0.54, 1.08 2.16, 4.32, and 10.8 J/cm2 were used. Treatment with light pulsating at 2.5 Hz, 6-16 weeks after X-irradiation, or treatment with light pulsating at 5 kHz, 4-16 weeks after X-irradiation, did not have a significant effect on the incidence or the latency for the development of ischemic dermal necrosis irrespective of the exposure time to light at each treatment. With light pulsating at 5 kHz, no effect of light dose was observed. However, the overall incidence of dermal necrosis was significantly reduced (P = 0.001) to 52% in the X-irradiated fields receiving treatment with 5 kHz light, 6-16 weeks after X-irradiation.(ABSTRACT TRUNCATED AT 250 WORDS)
Rezvani
M
M
CRC Normal Tissue Radiobiology Research Group, Research Institute (University of Oxford), Churchill Hospital, United Kingdom.
Nissan
M
M
Hopewell
J W
JW
van den Aardweg
G J
GJ
Robbins
M E
ME
Whitehouse
E M
EM
D000818:Animals; D005260:Female; D009336:Necrosis; D010789:Phototherapy; D011833:Radiation Injuries, Experimental / Q000517:prevention & control*; D012867:Skin / Q000473:pathology* / Q000528:radiation effects; D013552:Swine; D013997:Time Factors; D014965:X-Rays