Inositol stimulates DNA and protein synthesis, and expansion by rabbit blastocysts in vitro.
The effect of different concentrations (0, 0.6, 3, 15, 75 and 375 microM) of myo-inositol on the development of rabbit morulae to expanded blastocysts was investigated in terms of blastocyst expansion and synthesis of DNA and protein, as measured by incorporation of [3H]thymidine and [14C]amino acids into acid-precipitable material. A concentration of 15 microM inositol caused a 2.8-fold increase in blastocyst expansion (P less than 0.01), a 9.9-fold increase in thymidine incorporation into DNA (P less than 0.01) and a 3.6-fold increase in amino acid incorporation into protein (P less than 0.01). There were no significant differences in the range from 15 to 375 microM inositol.
Fahy
M M
MM
Department of Physiology, University College, Galway, Ireland.
Kane
M T
MT
D000818:Animals; D001755:Blastocyst / Q000187:drug effects* / Q000378:metabolism*; D004247:DNA / Q000096:biosynthesis*; D004305:Dose-Response Relationship, Drug; D066298:In Vitro Techniques; D007294:Inositol / Q000494:pharmacology*; D014176:Protein Biosynthesis; D011817:Rabbits
D007294:Inositol; D004247:DNA