Rapid high resolution western blotting: from gel to image in a single day.
A streamlined protocol is described that allows high sensitivity antigen detection by Western blotting in a single day. The choice of membrane blotting matrix, as well as blocking reagents, has been optimized in order to allow rapid development of the blot with chemiluminescent reagents. The entire process, from gel to blot to a permanent, hard copy image on x-ray film, can be accomplished within six hours.
Klapper
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A
Department of Cell Biology and Genetics, Sloan-Kettering Institute, New York, NY 10021.
MacKay
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B
Resh
M D
MD
D000818:Animals; D003411:Arvicolinae; D015153:Blotting, Western / Q000379:methods*; D002461:Cell Line, Transformed; D015688:Oncogene Protein pp60(v-src) / Q000032:analysis; D013997:Time Factors
D015688:Oncogene Protein pp60(v-src)