The protein of M(r) 21,000 constituting the prosome-like particle of duck erythroblasts is homologous to apoferritin.
In duck erythroblasts, two major populations of untranslated messenger (m) RNP can be separated by sucrose gradient centrifugation in low ionic strength. One of these contains globin mRNA associated to protein factors, among them the prosomes. The other, sedimenting in the 35S zone, contains non-globin mRNA. From this '35S' mRNP, a new RNP particle called the prosome-like particle was isolated and characterized [Akhayat, O., Infante, A. A., Infante, D., Martins de Sa, C., Grossi de Sa, M.-F. & Scherrer, K. (1987) Eur. J. Biochem. 170, 23-33]. The PLP is a multimer of a protein of M(r) 21,000, and contains small RNA species. The particle is tightly associated with repressed mRNA and inhibits in vitro protein synthesis. We show here that the protein of M(r) 21,000, constituting the prosome-like particle, is apoferritin. Different approaches confirm the RNP character of this particle and provide evidence that some of its RNA species are tRNA. The hypothesis is discussed as to whether (apo-)ferritin might serve other functions in addition to iron storage.
Coux
O
O
Institut J. MONOD, Paris, France.
Camoin
L
L
Nothwang
H G
HG
Bey
F
F
Silva Pereira
I
I
Keith
G
G
Strosberg
A D
AD
Scherrer
K
K
D000595:Amino Acid Sequence; D000818:Animals; D001052:Apoferritins / Q000235:genetics*; D001483:Base Sequence; D001798:Blood Proteins / Q000235:genetics*; D015152:Blotting, Northern; D015153:Blotting, Western; D002851:Chromatography, High Pressure Liquid; D004372:Ducks; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D005914:Globins / Q000235:genetics; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000378:metabolism; D012261:Ribonucleoproteins / Q000235:genetics
D001798:Blood Proteins; D012333:RNA, Messenger; D012261:Ribonucleoproteins; D005914:Globins; D001052:Apoferritins