Identification of chicken (Gallus domesticus) adipocyte plasma membrane and differentiation specific proteins using SDS-PAGE and western blotting.
1. Affinity-purified adipocyte membrane proteins were used to raise antisera in two sheep. 2. Using one of the antisera 15 proteins were identified as being adipocyte specific by comparison on Western blots of plasma membrane proteins from various tissues. 3. Of these 15 proteins eight appeared to be present only in mature adipocytes and not in the adipocyte precursor. 4. In the presence of guinea pig complement the two antisera raised were cytotoxic to adipocytes and their precursors. 5. Characterization and further study of these adipocyte differentiation specific proteins will provide valuable information about the process of adipocyte differentiation.
Butterwith
S C
SC
Agricultural and Food Research Council, Department of Cellular and Molecular Biology, Roslin, Midlothian, UK.
Kestin
S
S
Griffin
H D
HD
Beattie
J
J
Flint
D J
DJ
D000273:Adipose Tissue / Q000737:chemistry* / Q000166:cytology / Q000276:immunology; D000818:Animals; D015153:Blotting, Western; D002454:Cell Differentiation; D002478:Cells, Cultured; D002645:Chickens; D004591:Electrophoresis, Polyacrylamide Gel; D007106:Immune Sera; D008565:Membrane Proteins / Q000032:analysis*; D012756:Sheep
D007106:Immune Sera; D008565:Membrane Proteins