First isolation of gamma-L-glutamyl-gamma-aminobutyric acid from bovine brains.
We had obtained some immunohistochemical indications which suggested that peptide(s) containing GABA at the C-terminal could exist in specific neurons in brains. We therefore sought such GABA conjugate(s) by isolating an acidic GABA-dipeptide from bovine brains and determined its structure as gamma-L-glutamyl-gamma-aminobutyric acid (gamma-L-Glu-gamma Abu:GluGABA). Its gamma-linkage was determined by a mass-spectrometric (B/E linked scan SIMS) analysis; its chirality was established by a chiral column HPLC technique. This peptide is the first acidic GABA-peptide recognized in brain and the first brain peptide containing GABA at the C-terminal.
Yamamoto
A
A
Institute of Bio-Active Science (IBAS), Nippon Zoki Pharmaceutical Co., Ltd., Hyogo, Japan.
Ienaga
K
K
Nakamura
K
K
Nishimura
N
N
Higashiura
K
K
Kurimoto
Y
Y
Inoue
A
A
Kimurat
H
H
D000818:Animals; D001923:Brain Chemistry; D002417:Cattle; D002852:Chromatography, Ion Exchange; D004151:Dipeptides / Q000737:chemistry / Q000302:isolation & purification*; D013058:Mass Spectrometry; D015394:Molecular Structure
D004151:Dipeptides; C076487:gamma glutamyl GABA