Salicylate-induced cation fluxes across biological membranes. A study of the underlying mechanism.
The effect of salicylate on cation transport by lactating rat mammary tissue has been examined. Consistent with previous results, salicylate increased the unidirectional efflux of K+ (Rb+): this increase was dependent on extracellular Ca2+. Lowering the temperature of the incubation medium from 37 degrees to 4 degrees did not attenuate the effect of salicylate on K+ (Rb+). Ca2+ uptake by mammary tissue was stimulated by salicylate, whereas Ca2+ efflux was not greatly affected. Salicylate reduced the ouabain-insensitive uptake of K+ (Rb+). The net K+ content of mammary tissue was reduced by incubating tissue in a medium containing salicylate, whereas net Na+ content was increased. The results are consistent with the hypothesis that salicylate activated a calcium-dependent K+ efflux channel and/or a calcium-dependent non-selective cation channel.
Shennan
D B
DB
Hannah Research Institute, Ayr, U.K.
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002118:Calcium / Q000378:metabolism; D002412:Cations / Q000378:metabolism*; D002712:Chlorides / Q000494:pharmacology; D066298:In Vitro Techniques; D007774:Lactation; D008321:Mammary Glands, Animal / Q000378:metabolism*; D008566:Membranes / Q000378:metabolism; D011188:Potassium / Q000378:metabolism; D015221:Potassium Channels / Q000187:drug effects; D051381:Rats; D012459:Salicylates / Q000494:pharmacology*; D020156:Salicylic Acid; D012964:Sodium / Q000378:metabolism; D013696:Temperature
D002412:Cations; D002712:Chlorides; D015221:Potassium Channels; D012459:Salicylates; D012964:Sodium; D020156:Salicylic Acid; D011188:Potassium; D002118:Calcium