title: [Results of multicenter evaluation of reagents for determination of sodium, potassium and chloride ions using enzyme activation].
abstract: We present the results of a multicentre evaluation with Boehringer Mannheim/Hitachi instruments of new "enzymatic" methods for the determination of Na+, K+, and Cl- in serum or plasma. The between-day coefficient of variation was less than 1.4% (Na+), less than 2.6% (K+) and less than 1.7% (Cl-). The linear range of the assays were at least 80 to 200 mmol/l (Na+), 1.5 to 17 mmol/l (K+) and about 30 to at least 200 mmol/l (Cl-). The comparisons with routine flame atomic emission spectrometry and coulometry showed a satisfactory agreement of the test results. The "enzymatic" assays are insensitive to even grossly elevated levels of bilirubin and lipids (sodium, potassium, and chloride assays), NH4+ (potassium assay) and amylase (chloride assay). Interference by various drugs was not detected. Since the new methods can easily be adapted to photometric clinical chemistry instruments, they represent a valuable alternative to the use of ion-selective electrodes, flame atomic emission spectrometry and coulometry.
authors.affiliation: Boehringer Mannheim GmbH, Bundesrepublik Deutschland.
authors.forename: V
authors.initials: V
authors.lastname: Ehrhardt
authors.forename: H
authors.initials: H
authors.lastname: Baadenhuijsen
authors.forename: S
authors.initials: S
authors.lastname: Brenna
authors.forename: M
authors.initials: M
authors.lastname: Browne
authors.forename: L
authors.initials: L
authors.lastname: Garcia-Beltrán
authors.forename: K
authors.initials: K
authors.lastname: Hellsing
authors.forename: W R
authors.initials: WR
authors.lastname: Külpmann
authors.forename: K
authors.initials: K
authors.lastname: Paschen
authors.forename: C
authors.initials: C
authors.lastname: Pascual-Mostaza
authors.forename: P
authors.initials: P
authors.lastname: Pei
mesh_terms: D002138:Calibration; D002712:Chlorides / Q000097:blood*; D004789:Enzyme Activation / Q000502:physiology*; D005069:Evaluation Studies as Topic; D006801:Humans; D011188:Potassium / Q000097:blood*; D011786:Quality Control; D012964:Sodium / Q000097:blood*; D013053:Spectrophotometry / Q000295:instrumentation*
chemical_list: D002712:Chlorides; D012964:Sodium; D011188:Potassium
vernacular_title: Ergebnisse der multizentrischen Evaluierung von Reagenzien zur Bestimmung von Natrium-, Kalium- und Chloridionen mittels Enzymaktivierung.