title: A 16 kb small single-copy region separates the plastid DNA inverted repeat of the unicellular red alga Cyanidium caldarium: physical mapping of the IR-flanking regions and nucleotide sequences of the psbD-psbC, rps16, 5S rRNA and rpl21 genes.
abstract: The four inverted repeat (IR) flanking regions of the Cyanidium caldarium plastid DNA were cloned. Southern blotting, transcript and sequence analyses of the border regions revealed the psbD-psbC operon and the rps16 gene within the large single-copy region upstream of the 16S rDNA gene and the rpl21 gene downstream of the 5S rDNA within the 16 kb small single-copy region. The size of the IR is ca. 5 kb. The nucleotide sequences of the psbD-psbC, rps16, rpl21 and 5S rRNA genes with the corresponding alignments and physical maps of the regions are presented. Northern analysis revealed a less complex psbD-psbC transcription pattern than has been found in higher plants. Comparisons to other red algal data point to structural diversity within red algal plastid DNA.
authors.affiliation: Institut für Pflanzenphysiologie, Justus Liebig Universität, Giessen, Germany.
authors.forename: U
authors.initials: U
authors.lastname: Maid
authors.forename: K
authors.initials: K
authors.lastname: Zetsche
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D002736:Chloroplasts / Q000378:metabolism*; D004247:DNA / Q000378:metabolism; D008969:Molecular Sequence Data; D045322:Photosynthetic Reaction Center Complex Proteins / Q000235:genetics*; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D012341:RNA, Ribosomal, 5S / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D000461:Rhodophyta / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
chemical_list: D045322:Photosynthetic Reaction Center Complex Proteins; D012336:RNA, Ribosomal, 16S; D012341:RNA, Ribosomal, 5S; D004247:DNA