title: Plasma renin activity and aldosterone and vasopressin concentrations during incremental treadmill exercise in horses.
abstract: Six untrained mares were subjected to incremental treadmill exercise to examine exercise-induced changes in plasma renin activity (PRA) and plasma aldosterone (ALDO) and plasma arginine vasopressin (AVP) concentrations. Plasma renin activity, ALDO and AVP concentrations, and heart rate (HR) were measured at each step of an incremental maximal exercise test. Mares ran up a 6 degree slope on a treadmill set at an initial speed of 4 m/s. Speed was increased 1 m/s each minute until HR reached a plateau. Plasma obtained was stored at -80 C and later was thawed, extracted, and assayed for PRA and ALDO and AVP values by use of radioimmunoassay. Exercise caused significant increase in HR from 40 +/- 2 beats/min (mean +/- SEM) at rest to 206 +/- 4 beats/min (HRmax) at speed of 9 m/s. Plasma renin activity increased from 1.9 +/- 1.0 ng/ml/h at rest to a peak of 5.2 +/- 1.0 ng/ml/h at 9 m/s, paralleling changes in HR. Up to treadmill speed of 9 m/s, strong linear correlations were obtained between exercise intensity (and duration) and HR (r = 0.87, P less than 0.05) and PRA (r = 0.93, P less than 0.05). Heart rate and PRA reached a plateau and did not increase when speed was increased from 9 to 10 m/s. Plasma ALDO concentration increased from 48 +/- 16 pg/ml at rest to 191 +/- 72 pg/ml at speed of 10 m/s. Linear relation was found between exercise intensity (and duration) and ALDO concentration (r = 0.97, P less than 0.05).(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Veterinary Clinical Sciences, Ohio State University, Columbus 43210.
authors.forename: K H
authors.initials: KH
authors.lastname: McKeever
authors.forename: K W
authors.initials: KW
authors.lastname: Hinchcliff
authors.forename: L M
authors.initials: LM
authors.lastname: Schmall
authors.forename: S M
authors.initials: SM
authors.lastname: Reed
authors.forename: D R
authors.initials: DR
authors.lastname: Lamb
authors.forename: W W
authors.initials: WW
authors.lastname: Muir
mesh_terms: D000450:Aldosterone / Q000097:blood*; D000818:Animals; D001127:Arginine Vasopressin / Q000097:blood*; D005080:Exercise Test / Q000662:veterinary; D005260:Female; D006339:Heart Rate; D006736:Horses / Q000097:blood*; D005082:Physical Exertion / Q000502:physiology*; D012083:Renin / Q000097:blood*
chemical_list: D001127:Arginine Vasopressin; D000450:Aldosterone; D012083:Renin