Functional studies on long-term cryopreserved peripheral blood mononuclear cells from patients with lung cancer and from healthy subjects.
To determine the functional abilities of long-term cryopreserved peripheral blood mononuclear cells (PBMCs) from patients with lung cancer and from healthy subjects, we assayed the proliferative and plaque-forming cell (PFC) responses of cells from these individuals after the cells had been cryopreserved for up to 31 months. The stability of these cells for B and T cell quantitative assays was also determined by using their respective monoclonal antibodies. The patients' results were compared with those in healthy subjects to ascertain whether the conclusions derived from the assay of cryopreserved cells are consistent with our earlier studies on fresh cells from similar patients. The results show that proliferative and PFC responses of the frozen cells were not significantly affected by further storage, despite an initial, irreversible functional loss in some subsets of T lymphocytes and monocytes during the process of freezing. They also demonstrate that cryopreserved PBMCs from both patients and controls can be successfully utilized for B and T cell quantitative assays. The conclusions derived from the assay of cryopreserved cells are also consistent with our earlier observations on fresh cells from patients with lung cancer; those studies indicated a B cell functional abnormality caused in part by increased suppressor T cell and monocyte activity.
Venkataraman
M
M
Department of Medicine, Mount Sinai Hospital Medical Center, Chicago, IL 60608.
Rao
D S
DS
Westerman
M P
MP
D000368:Aged; D002455:Cell Division; D015925:Cryopreservation; D005260:Female; D006462:Hemolytic Plaque Technique; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000502:physiology*; D008175:Lung Neoplasms / Q000097:blood; D016131:Lymphocyte Subsets; D008297:Male; D008875:Middle Aged