Detection of HIV-1 genome in leukocytes of human colostrum from anti-HIV-1 seropositive mothers.
In order to obtain more information about the presence of HIV-1 in mononuclear cells of colostrum, research was carried out on both the HIV-1 genome in the cellular fraction of colostrum and the viral antibody in cell-free colostrum of eight anti-HIV-1 seropositive asymptomatic mothers. In five cases cell fractions of the colostrum harbored HIV-1 genome by DNA-DNA and DNA-RNA in situ hybridization, whereas viral antibody were detected in all cell-free colostrum specimens. The data confirms the colostrum as a possible route of HIV-1 infection.
Vonesch
N
N
Institute of Infectious Diseases, University of Rome La Sapienza, Italy.
Sturchio
E
E
Humani
A C
AC
Cordiali Fei
P
P
Cosenza
D
D
Caprilli
F
F
Pezzella
M
M
D000328:Adult; D003126:Colostrum / Q000382:microbiology*; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D016679:Genome, Viral; D015483:HIV Antibodies / Q000032:analysis; D006679:HIV Seropositivity / Q000276:immunology / Q000382:microbiology*; D015497:HIV-1 / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D007231:Infant, Newborn; D007962:Leukocytes / Q000382:microbiology*; D009035:Mothers; D009693:Nucleic Acid Hybridization; D012367:RNA, Viral / Q000032:analysis
D004279:DNA, Viral; D015483:HIV Antibodies; D012367:RNA, Viral