title: Electron-microscopic study of the effect of various extractants on the morphology of Coxiella burnetii.
abstract: This paper presents the results of the effect of trichloroacetic acid (TCA) at two different temperatures and the effect of a mixture of detergents (D) on Coxiella burnetii. Both TCA and D caused a large destruction of C. burnetii cells. In both cases complexes of high-molar-mass components from the outer membrane were extracted. In the D case not only proteins but a mixture with other high-molar-mass structures were released from the destroyed cells. By extraction of TCA, the antigenic complex composed of lipopolysaccharides (LPS), proteins and phospholipids was released. The effect of the D mixture on C. burnetii causes their complete destruction. The TCA causes a surface destruction of cells but it does not cause complete disintegration. The small-cell variants (SCV) in both cases were shown to be more stable compared to the large-cell variants (LCV).
authors.affiliation: Institute of Virology, Slovak Academy of Sciences, Bratislava, Czechoslovakia.
authors.forename: M
authors.initials: M
authors.lastname: Vavreková
authors.forename: F
authors.initials: F
authors.lastname: Ciampor
authors.forename: M
authors.initials: M
authors.lastname: Lukácová
mesh_terms: D000942:Antigens, Bacterial / Q000302:isolation & purification; D016997:Coxiella burnetii / Q000187:drug effects / Q000276:immunology / Q000648:ultrastructure*; D003902:Detergents / Q000494:pharmacology*; D008854:Microscopy, Electron; D012995:Solubility; D013696:Temperature; D014238:Trichloroacetic Acid / Q000494:pharmacology*
chemical_list: D000942:Antigens, Bacterial; D003902:Detergents; D014238:Trichloroacetic Acid