Molecular analysis of a gene from Bacteroides fragilis involved in metronidazole resistance in Escherichia coli.
The region of Bacteroides fragilis DNA on the recombinant plasmid pMT100 responsible for conferring metronidazole resistance in Escherichia coli strains was characterized. An open reading frame (ORF1) of 195 bp encoded a protein of 64 amino acids with a predicted M(r) of 7.3 kDa. Deletion analysis indicated that ORF1 conferred the metronidazole resistance phenotype and encoded a protein with an apparent M(r) of approximately 8-10 kDa.
Wehnert
G U
GU
Department of Microbiology, University of Cape Town, Rondebosch, South Africa.
Abratt
V R
VR
Woods
D R
DR
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001441:Bacteroides fragilis / Q000187:drug effects / Q000235:genetics*; D001483:Base Sequence; D004352:Drug Resistance, Microbial / Q000235:genetics; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics; D005798:Genes, Bacterial; D008795:Metronidazole / Q000494:pharmacology*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D011993:Recombinant Fusion Proteins / Q000378:metabolism
D001426:Bacterial Proteins; D011993:Recombinant Fusion Proteins; D008795:Metronidazole