Mapping of intracellular halogenous molecules by low and high resolution SIMS microscopy.
The subcellular distribution of halogenous molecules has been studied by SIMS microscopy in cultured cells of a human breast carcinoma (MCF-7 cell line). Two instruments of microanalysis were used. A low lateral resolution ion microscope (SMI 300 CAMECA) and a prototype scanning ion microscope equipped with a cesium gun that gives high lateral resolution images. This apparatus has been developed by G Slodzian, in Onera Laboratories (Office National d'Etudes et de Recherches Aérospatiales). Molecules studied by low lateral resolution ion microscope were halogenous steroids: fluorometholone, triamcinolone, bromocriptine and bromoandrosterone. Analytical images show that the first two compounds are mainly localized in the nuclear structure of MCF-7 cells whereas the last two molecules are localized in cytoplasm of these cells. Images were obtained with a resolution of 1 micron. With the scanning ion microscope, it is now possible to obtain images at the ultrastructural level. Four analytical images can be simultaneously obtained by a single scan of the imaged area, corresponding to a depth of erosion of the section of ten nm. The intranuclear distributions of three pyrimidine analogs, 5-bromo-2'-deoxyuridine, 5-iodo-2'-deoxyuridine and 5-fluorouracil have been studied in phase S and M of MCF-7 cells and these images have been compared to the distribution of sulfur, nitrogen and phosphorus. All these images have been obtained with a lateral resolution better than 100 nm.
Berry
J P
JP
SC27 de l'Inserm, Faculté de Médecine, Créteil, France.
Galle
P
P
Chassoux
D
D
Escaig
F
F
Linarez-Cruz
L G
LG
Lespinats
G
G
D000305:Adrenal Cortex Hormones / Q000032:analysis; D000728:Androgens / Q000032:analysis; D001943:Breast Neoplasms / Q000378:metabolism; D001971:Bromocriptine / Q000032:analysis; D001973:Bromodeoxyuridine; D002478:Cells, Cultured; D005469:Fluorometholone / Q000032:analysis; D006219:Halogens / Q000032:analysis*; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D008853:Microscopy / Q000379:methods*; D008938:Mitosis; D009711:Nucleotides / Q000032:analysis; D014221:Triamcinolone / Q000032:analysis; D014407:Tumor Cells, Cultured
D000305:Adrenal Cortex Hormones; D000728:Androgens; D006219:Halogens; D009711:Nucleotides; D014221:Triamcinolone; D001971:Bromocriptine; D001973:Bromodeoxyuridine; D005469:Fluorometholone