[Inhibitory effect of ulinastatin on the alpha-thrombin activation of factors V and VIII].
Ulinastatin is a remedy of urine serinprotease inhibitors and also it inhibits enzymatic activities of several blood coagulation factors. The action of ulinastatin on alpha-thrombin shows noncompetitive inhibition with the formation of enzyme-inhibitor complex. In this study, the effects of ulinastatin on the alpha-thrombin activation of factors V and VIII are observed by 5-20% gradient gel SDS PAGE with or without Western blotting method. The addition of ulinastatin to the mixture of factor V and alpha-thrombin inhibits the production of active peptides proceeded in the alpha-thrombin activation process of factor V. Furthermore, using anti-factor VIII 43KDa peptide monoclonal antibody with Western blotting method, the addition of ulinastatin to the mixture of alpha-thrombin and factor VIII indicates to inhibit the release of 43KDa peptide from factor VIII in the process of factor VIII activation induced by alpha-thrombin.
Mimura
K
K
Toyo Public Health College, Tokyo.
Shinozawa
K
K
Kobayashi
T
T
Fukutake
K
K
Yatsuhashi
T
T
Fukutake
K
K
D005165:Factor V / Q000378:metabolism*; D005169:Factor VIII / Q000378:metabolism*; D006023:Glycoproteins / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D013917:Thrombin / Q000037:antagonists & inhibitors*
D006023:Glycoproteins; D005165:Factor V; D005169:Factor VIII; D013917:Thrombin; C028665:urinastatin