Enzyme-linked immunosorbent assay for the detection of Fusobacterium necrophorum antibody in bovine sera.
An enzyme-linked immunosorbent assay (ELISA) with HCl heat extracted antigen of Fusobacterium necrophorum was developed for the detection of antibody in bovine sera. Optimal conditions for antigen concentration and dilution of bovine serum were established. Pretreatment of positive reference serum with the antigens of different bacteria demonstrated no decrease, whereas the serum pretreated with F. necrophorum antigens revealed a decrease in the ELISA values. The apparent difference in ELISA values was observed between the sera derived from cattle infected and not infected with Fusobacterium necrophorum. These findings indicate that the ELISA detects the antibody to F. necrophorum in bovine sera.
Kameyama
Y
Y
Department of Veterinary Microbiology, Faculty of Agriculture, Yamaguchi University, Japan.
Kanoe
M
M
Kai
K
K
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D000942:Antigens, Bacterial; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005674:Fusobacterium Infections / Q000276:immunology / Q000662:veterinary*; D005675:Fusobacterium necrophorum / Q000276:immunology*
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial