Cleavage of type VIII collagen by human neutrophil elastase.
In this report, the susceptibility of type VIII collagen to human neutrophil elastase is compared to other extracellular matrix components. Type X collagen is degraded to specific fragments at a substrate to enzyme ratio of 5:1 after 20 h at room temperature, but type VIII collagen is almost completely degraded after only 4 h incubation at a substrate to enzyme ratio of 50:1 and partly degraded after only 15 min. Laminin, merosin and types I, III, IV and V collagen exhibit no susceptibility to neutrophil elastase under the latter conditions, while fibronectin is degraded.
Kittelberger
R
R
Wallaceville Animal Research Centre, Upper Hutt, New Zealand.
Neale
T J
TJ
Francky
K T
KT
Greenhill
N S
NS
Gibson
G J
GJ
D003094:Collagen / Q000737:chemistry / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D005353:Fibronectins / Q000378:metabolism; D006801:Humans; D007797:Laminin / Q000378:metabolism; D019272:Leukocyte Elastase; D008565:Membrane Proteins / Q000378:metabolism; D010196:Pancreatic Elastase / Q000378:metabolism*; D013379:Substrate Specificity; D013696:Temperature
D005353:Fibronectins; D007797:Laminin; D008565:Membrane Proteins; D003094:Collagen; D010196:Pancreatic Elastase; D019272:Leukocyte Elastase