Polyclonal antibodies against Staphylococcus aureus ATCC 12600 catalase do not recognize any protein in cellular extracts from S. aureus subsp. anaerobius.
Rabbits were immunized with electrophoretically pure catalase from Staphylococcus aureus ATCC 12 600. The antiserum was used to study whether S. aureus subsp. anaerobius was able to synthesize the apoprotein of catalase. Proteins were separated on polyacrylamide gels (SDS-PAGE) and transferred to nitrocellulose membranes and were detected by immunoblotting. No protein reacting with the purified immunoglobulins against S. aureus ATCC 12,600 catalase could be detected in crude and partially purified cellular extracts from S. aureus subsp. anaerobius or its aerotolerant mutants.
Ruiz Santa Quiteria
J A
JA
Departamento Patología Animal I, Facultad de Veterinaria, Universidad Complutense, Madrid, Spain.
Cid
D
D
Bellahsene
R
R
Suárez
G
G
de la Fuente
R
R
D020642:Acatalasia; D000907:Antibodies, Bacterial / Q000737:chemistry*; D000918:Antibody Specificity; D001426:Bacterial Proteins / Q000276:immunology*; D002374:Catalase / Q000276:immunology* / Q000302:isolation & purification; D013045:Species Specificity; D013211:Staphylococcus aureus / Q000145:classification / Q000201:enzymology* / Q000276:immunology; D013347:Subcellular Fractions / Q000201:enzymology
D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D002374:Catalase