title: Serum and urinary ceruloplasmin in experimental nephrotic syndrome.
abstract: The levels of ceruloplasmin (Cp) and total protein were measured in serum and urine from rats with experimental nephrotic syndrome (NS) for 20 days after a single injection of puromycin aminonucleoside (PAN). Control values for Cp in serum and urine were: 0.23 +/- 0.01 mg/ml and 0.063 mg/day, and those for total protein were: 7.1 +/- 0.14 g/dl and 2.1 +/- 0.6 mg/day, respectively. It was found that: a) serum Cp decreased on day 6, remained low until day 10 (0.07 +/- 0.01 to 0.08 +/- 0.01 mg/ml), and returned to control levels on day 12; b) total serum protein decreased on day 4, reached the lowest value on day 6 (3.3 +/- 0.3 g/dl) and returned to control levels on day 16; c) Cp in urine increased on day 6, reached a peak value on day 8 (0.62 +/- 0.07 mg/day), then decreased but remained high on day 20; and d) total protein in urine increased on day 5, reached a peak value on day 10 (692 +/- 59 mg/day) and returned to control values on day 20. There was a close association between the levels of Cp and total protein in serum and urine. The decrease of circulating Cp indicates that a typical acute phase response does not occur in the PAN-nephrotic rats. The loss of Cp in the urine of the nephrotic rats may contribute to the decrease in the serum levels of this protein.
authors.affiliation: Departamento de Biología, Facultad de Ciencias, Universidad Nacional Autónoma de México, D.F.
authors.forename: A
authors.initials: A
authors.lastname: Mainero
authors.forename: C
authors.initials: C
authors.lastname: Cruz
authors.forename: J
authors.initials: J
authors.lastname: Pedraza-Chaverrí
mesh_terms: D000818:Animals; D001798:Blood Proteins / Q000378:metabolism; D002570:Ceruloplasmin / Q000378:metabolism* / Q000652:urine; D007700:Kinetics; D008297:Male; D009404:Nephrotic Syndrome / Q000139:chemically induced / Q000378:metabolism*; D011507:Proteinuria / Q000652:urine; D011692:Puromycin Aminonucleoside; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D001798:Blood Proteins; D011692:Puromycin Aminonucleoside; D002570:Ceruloplasmin