Glutathione depletion and rabbit renal medulla 6-ketoPGF1 alpha and TxB2: levels in vivo and following homogenate incubation in vitro.
1. The effect of glutathione (GSH) depletion on rabbit renal medullary homogenate 6-ketoPGF1 alpha and TxB2 synthesizing capability was investigated. 2. GSH depletion in vivo with diethyl maleate (DEM) produced higher (P less than 0.05) 6-ketoPGF1 alpha and TxB2 renal medullary levels compared to controls. Homogenization and incubation lowered (P less than 0.05) GSH such that there were no differences in GSH between treatments after 5 min of incubation. By 30 min, GSH was lower (P less than 0.05) and 6-ketoPGF1 alpha higher (P less than 0.05) in homogenates from controls in comparison to those from DEM-treated rabbits. 3. The results indicate GSH depletion increased 6-ketoPGF1 alpha levels in rabbit renal medulla in vivo but subsequent GSH catabolism prevented assessing the effect of this GSH depletion on prostanoid synthesizing capability.
Nejad
H H
HH
Department of Animal and Poultry Sciences, University of Arkansas, Fayetteville 72701.
Bottje
W G
WG
D015121:6-Ketoprostaglandin F1 alpha / Q000378:metabolism*; D000818:Animals; D005978:Glutathione / Q000378:metabolism*; D066298:In Vitro Techniques; D007679:Kidney Medulla / Q000378:metabolism*; D008297:Male; D008298:Maleates; D010084:Oxidation-Reduction; D011817:Rabbits; D011863:Radioimmunoassay; D013929:Thromboxane B2 / Q000378:metabolism*
D008298:Maleates; D013929:Thromboxane B2; D015121:6-Ketoprostaglandin F1 alpha; C014476:diethyl maleate; D005978:Glutathione