title: Epithelial-mesenchymal interactions in murine organogenesis.
abstract: Reciprocal, sequential interactions between embryonic epithelial and their mesenchymal stroma guide the cytodifferentiation and organization of both components. These morphogenetic interactions and their consequences are examined in two model systems in vitro: the mouse metanephric blastema and the tooth rudiment. Experimental approaches include dissection and recombination of the interacting tissues, localization of molecular changes by immunohistology and in situ hybridization. An early response of the mesenchyme is increased proliferation of cells in the vicinity of the epithelial inductor and their subsequent aggregation (condensation). In the kidney model disruption of this aggregation or prevention of assembly of the programmed cells results in impaired cytodifferentiation. If the cells are allowed to reaggregate, a phenotype is expressed not unlike that seen in normal in vivo conditions. Our present interest is focused on the early metabolic events associated with the condensation phenomenon. The cell surface proteoglycan syndecan and the matrix glycoprotein tenascin are expressed in the condensed mesenchyme and may mediate cell-matrix interactions. The expression patterns of certain growth factors suggest functions in signal transduction.
authors.affiliation: Department of Pathology, University of Helsinki, Finland.
authors.forename: L
authors.initials: L
authors.lastname: Saxén
authors.forename: I
authors.initials: I
authors.lastname: Thesleff
mesh_terms: D000818:Animals; D002449:Cell Aggregation / Q000502:physiology; D002450:Cell Communication / Q000502:physiology*; D005314:Embryonic and Fetal Development / Q000502:physiology*; D004847:Epithelial Cells; D008648:Mesoderm / Q000166:cytology*; D051379:Mice; D015398:Signal Transduction / Q000502:physiology