Species-differences in the pH-activity pattern of acid phosphatase in the family Enterobacteriaceae.
Nonspecific phosphatase activities were surveyed comparing major species of the family Enterobacteriaceae. The strains were subjected to a whole cell assay system with paranitrophenyl phosphate as substrate over a wide pH range and with a standardized number of bacterial cells. The overall results suggest that the general shape of the pH activity curve and the location of peaks (pH optimum) can be employed as a supplementary criterion to characterize species of Enterobacteriaceae.
Dejsirilert
S
S
National Institute of Health, Department of Medical Sciences, Nonthaburi, Thailand.
Kondo
E
E
Kanai
K
K
D000135:Acid Phosphatase / Q000502:physiology*; D015373:Bacterial Typing Techniques / Q000592:standards; D001681:Biological Assay; D004755:Enterobacteriaceae / Q000145:classification / Q000201:enzymology*; D005069:Evaluation Studies as Topic; D006801:Humans; D006863:Hydrogen-Ion Concentration; D009596:Nitrophenols; D009943:Organophosphorus Compounds; D013045:Species Specificity; D013997:Time Factors
D009596:Nitrophenols; D009943:Organophosphorus Compounds; C008644:nitrophenylphosphate; D000135:Acid Phosphatase