title: Two variants of transferrable extended-spectrum TEM-beta-lactamase successively isolated from a clinical Escherichia coli isolate.
abstract: In a leukaemic patient presenting a septicaemia treated with ceftazidime and amikacin, two clinical Escherichia coli isolates distinguished by their level of resistance to oxyimino-beta-lactams were isolated at an interval of 24 h. The isolates were identified by biotyping and esterase electrophoretic typing and the two host strains were shown to be identical. However, each of these strains exhibited a different transferrable extended-spectrum beta-lactamase. These enzymes had different pI values (5.25 and 5.58), but were both blaTEM-1 mutants. The enzyme with pI 5.25 was identical to TEM-101 (TEM-12) (serine 162 substitution). The enzyme with pI 5.58 showed an additional amino acid substitution (lysine residue instead of an arginine at position 237) and was denominated TEM-23. These data indicate that point-mutations can be successively cumulated in vivo by blaTEM mutants, leading to expression of beta-lactamases with increased hydrolysis rates.
authors.affiliation: CHU Cochin, Laboratoire de Bactériologie, Paris, France.
authors.forename: G
authors.initials: G
authors.lastname: Vedel
authors.forename: C
authors.initials: C
authors.lastname: Mabilat
authors.forename: S
authors.initials: S
authors.lastname: Goussard
authors.forename: B
authors.initials: B
authors.lastname: Picard
authors.forename: G
authors.initials: G
authors.lastname: Fournier
authors.forename: L
authors.initials: L
authors.lastname: Gilly
authors.forename: G
authors.initials: G
authors.lastname: Paul
authors.forename: A
authors.initials: A
authors.lastname: Philippon
mesh_terms: D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000302:isolation & purification; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000145:classification / Q000187:drug effects / Q000201:enzymology*; D006801:Humans; D007527:Isoenzymes / Q000235:genetics* / Q000302:isolation & purification; D007938:Leukemia / Q000382:microbiology; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D013379:Substrate Specificity; D001618:beta-Lactamases / Q000096:biosynthesis / Q000145:classification / Q000302:isolation & purification*
chemical_list: D004269:DNA, Bacterial; D007527:Isoenzymes; D001618:beta-Lactamases