title: The analysis and identification of steroids.
abstract: In October 1987, anabolic steroids were controlled under Schedule IV of Florida State Statute 893. This study was designed to establish a method of analysis and identification of evidentiary cases. Spot tests, Fourier transform infrared spectroscopy (FT-IR), gas chromatography/mass spectroscopy (GC/MS), Fourier transform nuclear magnetic resonance (FT-NMR), thin-layer chromatography (TLC), and extractions were performed on standard and pharmaceutical steroids. Mandelin's accompanied by the sulfuric acid test was found to provide the best indication of a steroid among the following four spot tests performed: sulfuric acid, napthol-sulfuric acid, Liebermann's, and Mandelin's. TLC was successfully performed on the steroid samples using two different eluent systems: TP and TQ. GC/MS was a very useful method of analysis applicable to most steroids, with only a few exceptions. FT-IR spectra were found to match the spectra from the literature for all the standards tested. The combination of the above techniques was used successfully to identify all the standards tested.
authors.affiliation: Metro-Dade Police Department, Crime Laboratory Bureau/Analytical Section, Miami, FL.
authors.forename: D M
authors.initials: DM
authors.lastname: Chiong
authors.forename: E
authors.initials: E
authors.lastname: Consuegra-Rodriguez
authors.forename: J R
authors.initials: JR
authors.lastname: Almirall
mesh_terms: D045930:Anabolic Agents / Q000032:analysis*; D002855:Chromatography, Thin Layer; D008401:Gas Chromatography-Mass Spectrometry; D009682:Magnetic Resonance Spectroscopy; D013055:Spectrophotometry, Infrared; D013256:Steroids / Q000032:analysis*
chemical_list: D045930:Anabolic Agents; D013256:Steroids