title: A useful modification of cDNA that enhances purification of recombinant protein.
abstract: With the availability of new and improved DNA vectors for producing large amounts of proteins by recombinant DNA technology, there is a growing need for efficient recovery of the desired protein, often from liters of culture medium. In this report, we describe a relatively simple modification of recombinant cDNA, that, in combination with immobilized metal affinity chromatography, is a rapid, inexpensive and highly effective method for enriching or purifying a desired protein.
authors.affiliation: Molecular Diagnostics, Inc., West Haven, CT 06516.
authors.forename: L
authors.initials: L
authors.lastname: Drake
authors.forename: T
authors.initials: T
authors.lastname: Barnett
mesh_terms: D001483:Base Sequence; D002846:Chromatography, Affinity; D003062:Codon; D004274:DNA, Recombinant / Q000235:genetics*; D006639:Histidine / Q000235:genetics; D007098:Imino Acids; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000302:isolation & purification*; D012685:Sepharose / Q000031:analogs & derivatives
chemical_list: D003062:Codon; D004274:DNA, Recombinant; D007098:Imino Acids; D011994:Recombinant Proteins; C042660:zinc(II)-iminodiacetate agarose; D006639:Histidine; D012685:Sepharose