title: Stereoselective conjugation of a uricosuric diuretic with glutathione by glutathione transferase 3-3.
abstract: 1. The activity of glutathione (GSH) transferases in rat liver cytosol was inhibited by the (-) enantiomer of a uricosuric diuretic (6,7-dichloro-5[N,N-dimethylsulphamoyl]-2,3-dihydrobenzofuran-2-ca rboxylic acid, DBCA) in a concentration-dependent manner. Although the DBCA (+) enantiomer inhibited the activity of liver cytosol GSH transferases, it was less effective. 2. Among four purified GSH transferase isozymes obtained from rat liver cytosol, isozyme 3-3 showed stereoselective interactions with the enantiomers of DBCA. This isozyme most actively and preferentially catalysed the transfer of GSH to DBCA (-) enantiomer.
authors.affiliation: Department of Pharmacology, Faculty of Pharmaceutical Sciences, Kobe-Gakuin University, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Kariya
authors.forename: H
authors.initials: H
authors.lastname: Okumura
authors.forename: E
authors.initials: E
authors.lastname: Lee
authors.forename: M
authors.initials: M
authors.lastname: Hirata
mesh_terms: D000818:Animals; D003600:Cytosol / Q000201:enzymology; D004232:Diuretics / Q000378:metabolism* / Q000494:pharmacology; D005978:Glutathione / Q000378:metabolism*; D005982:Glutathione Transferase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D066298:In Vitro Techniques; D007527:Isoenzymes / Q000037:antagonists & inhibitors / Q000378:metabolism; D008099:Liver / Q000201:enzymology; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013237:Stereoisomerism; D013449:Sulfonamides / Q000378:metabolism* / Q000494:pharmacology
chemical_list: D004232:Diuretics; D007527:Isoenzymes; D013449:Sulfonamides; C052960:S 8666; D005982:Glutathione Transferase; D005978:Glutathione