Immunohistochemical localization of hyaluronan synthase in cornea and conjunctive of cynomolgus monkey.
Distribution of hyaluronan synthase was investigated in cornea and conjunctiva of Cynomolgus monkeys (Macaca fascicularis) using polyclonal antibodies against the streptococcal enzyme. Strong immunoreaction was found in the cell membranes of the corneal endothelium, corneal epithelium, and most of the conjunctival epithelium. In the corneal epithelium all cells except the basal ones stained. In the conjunctiva all cylindrical cells stained, whereas among the goblet cells one type showed intense membrane staining, the other remained unstained. In the limbal portion of the conjunctival epithelium, which in many other respects differs morphologically and functionally from the remaining conjunctiva, all membranes of the different layers of the stratified epithelium except the most superficial ones, appeared unstained. Staining was also seen in all stromal fibroblasts and capillary endothelial cells.
Rittig
M
M
Department of Anatomy, University of Erlangen-Nürnberg, Germany.
Lütjen-Drecoll
E
E
Prehm
P
P
D000818:Animals; D003228:Conjunctiva / Q000201:enzymology*; D003315:Cornea / Q000201:enzymology*; D004848:Epithelium / Q000201:enzymology; D014453:Glucuronosyltransferase / Q000032:analysis*; D016695:Glycosyltransferases; D000076002:Hyaluronan Synthases; D007124:Immunoenzyme Techniques; D008252:Macaca fascicularis; D008297:Male; D008565:Membrane Proteins; D014166:Transferases; D029867:Xenopus Proteins
D008565:Membrane Proteins; D029867:Xenopus Proteins; D014166:Transferases; D016695:Glycosyltransferases; D014453:Glucuronosyltransferase; C056944:HAS1 protein, Xenopus; D000076002:Hyaluronan Synthases