title: Membrane inserted APP fragments containing the beta A4 sequence of Alzheimer's disease do not aggregate.
abstract: Previously we have shown that the COOH-terminal fragment (A4CT) of the Alzheimer amyloid protein precursor (APP), which at the NH2-terminus carries the sequence of the amyloid beta A4 protein, forms highly insoluble aggregates [EMBO J. (1988) 7, 949-957]. Here we report that aggregation is prevented if A4CT is expressed in vitro with a signal sequence at the NH2-terminus (SPA4CT) under conditions which allow membrane insertion. Aggregates from SPA4CT are obtained after removal of membranes by chloroform/methanol extraction or heating.
authors.affiliation: Center for Molecular Biology, University of Heidelberg, Germany.
authors.forename: T
authors.initials: T
authors.lastname: Dyrks
authors.forename: E
authors.initials: E
authors.lastname: Dyrks
authors.forename: C
authors.initials: C
authors.lastname: Masters
authors.forename: K
authors.initials: K
authors.lastname: Beyreuther
mesh_terms: D000544:Alzheimer Disease / Q000378:metabolism*; D000595:Amino Acid Sequence; D016564:Amyloid beta-Protein Precursor / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D004285:Dogs; D006801:Humans; D007425:Intracellular Membranes / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism; D008861:Microsomes / Q000378:metabolism; D008969:Molecular Sequence Data; D010957:Plasmids; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015183:Restriction Mapping; D014158:Transcription, Genetic
chemical_list: D016564:Amyloid beta-Protein Precursor; D008565:Membrane Proteins; D012333:RNA, Messenger