Controlled release of proteins and peptides from hydrogels synthesized by gamma ray-induced polymerization.
Hydrogels prepared by radiation-induced polymerization at a low temperature have been used as carriers for the controlled release of peptides and proteins. It was found that polymerization of 2-hydroxyethyl methacrylate in the presence of poly (ethylene glycol) methyl ether (MPEG) enabled the more porous and swellable matrics to be obtained, the higher the molecular weight of MPEG. As a consequence, protein release took place at an increasing extent and, provided that MPEG molecular weight was high enough, high molecular weight proteins could also be released. Such a state of affairs was not met in the case of hydrogels based on poly (2-hydroxyethyl acrylate). SEM analysis revealed that even high molecular weight MPEG did not give rise to any porosity, even though the degree of swelling was very high. As a result, no protein release was observed. It was therefore concluded that control of hydrogel porosity for the controlled release of large proteins is of overwhelming importance.
Caliceti
P
P
Dipartimento di Scienze Farmaceutiche (Centro di Studio di Chimica del Farmaco e dei Prodotti Biologicamente Attivi, C.N.R.), Università di Padova, Italy.
Veronese
F
F
Schiavon
O
O
Lora
S
S
Carenza
M
M
D000426:Alcohol Dehydrogenase / Q000737:chemistry; D005720:Gamma Rays; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D008689:Methacrylates / Q000737:chemistry; D008854:Microscopy, Electron; D008970:Molecular Weight; D009113:Muramidase / Q000737:chemistry; D010455:Peptides / Q000008:administration & dosage* / Q000737:chemistry; D011092:Polyethylene Glycols / Q000138:chemical synthesis* / Q000528:radiation effects; D011108:Polymers / Q000138:chemical synthesis / Q000528:radiation effects; D011506:Proteins / Q000008:administration & dosage* / Q000737:chemistry; D012260:Ribonucleases / Q000737:chemistry; D012709:Serum Albumin / Q000737:chemistry
D008689:Methacrylates; D010455:Peptides; D011108:Polymers; D011506:Proteins; D012709:Serum Albumin; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D011092:Polyethylene Glycols; C005044:hydroxyethyl methacrylate; D000426:Alcohol Dehydrogenase; D012260:Ribonucleases; D009113:Muramidase