v-Src induces elevated levels of diglyceride by stimulation of phosphatidylcholine hydrolysis.
When Rat-1 cells bearing the ts LA29 mutant of Rous sarcoma virus (Rat1 LA29) are shifted from restrictive to permissive temperature, the pp60v-Src tyrosine kinase is activated and there is an increase in the cellular level of sn1,2-diacylglycerol (DRG) within 30 min which is not accompanied by increased inositol phospholipid hydrolysis. Temperature shift also increases the hydrolysis of phosphatidylcholine (PC), as determined by an increase in the generation of water soluble choline metabolites. Transphosphatidylation studies have shown that this occurs at least in part via a phospholipase D (PLD) catalysed pathway.
Wyke
A W
AW
Beatson Institute For Cancer Research, Cancer Research Campaign Laboratories, Bearsden, Glasgow, Scotland, U.K.
Cook
S J
SJ
MacNulty
E E
EE
Wakelam
M J
MJ
D000818:Animals; D002460:Cell Line; D004075:Diglycerides / Q000032:analysis / Q000096:biosynthesis*; D004789:Enzyme Activation; D016391:Genes, src / Q000502:physiology*; D010712:Phosphatidic Acids / Q000502:physiology; D010713:Phosphatidylcholines / Q000378:metabolism*; D010739:Phospholipase D / Q000502:physiology; D011505:Protein-Tyrosine Kinases / Q000502:physiology*; D051381:Rats; D013696:Temperature
C011439:1,2-diacylglycerol; D004075:Diglycerides; D010712:Phosphatidic Acids; D010713:Phosphatidylcholines; D011505:Protein-Tyrosine Kinases; D010739:Phospholipase D