title: Interleukin-1 from baboon peripheral blood monocytes: altered response to endotoxin (lipopolysaccharide) and Staphylococcus aureus stimulation compared with human monocytes.
abstract: The baboon Papio ursinus does not elicit a febrile response upon injection with endotoxin, but fever is produced when injected with Staphylococcus aureus particles (Zurowsky, Y., H. Laburn, D. Mitchell, Can. J. Physiol. Pharmacol. 65, 1402-1407 (1987)). We address the question whether baboon peripheral blood monocytes produce interleukin-1 (IL-1) when stimulated with endotoxin or S. aureus particles in culture. Results show that little IL-1 biological activity was produced from endotoxin-stimulated baboon peripheral blood monocytes, compared with S. aureus-stimulated cells. Measurements of IL-1 beta by radioimmunoassay supported these data. This is contrary to data from human monocytes, which show greater sensitivity to endotoxin. Examination of IL-1 beta mRNA from endotoxin-stimulated and S. aureus-stimulated baboon monocytes, however, showed that more mRNA for IL-1 beta was present in endotoxin-stimulated monocytes than in cells stimulated with S. aureus. This illustrates the possibility that the production and/or the secretion of IL-1 beta is not as efficient in baboon monocytes as it is in human monocytes.
authors.affiliation: Department of Medical Biochemistry, University of the Witwatersrand Medical School, Parktown, Johannesburg/South Africa.
authors.forename: G U
authors.initials: GU
authors.lastname: von Bülow
authors.forename: A J
authors.initials: AJ
authors.lastname: Puren
authors.forename: N
authors.initials: N
authors.lastname: Savage
mesh_terms: D000818:Animals; D001681:Biological Assay; D015152:Blotting, Northern; D006801:Humans; D007375:Interleukin-1 / Q000096:biosynthesis* / Q000235:genetics; D008070:Lipopolysaccharides / Q000633:toxicity*; D009000:Monocytes / Q000187:drug effects* / Q000378:metabolism; D010215:Papio; D012333:RNA, Messenger / Q000032:analysis; D011863:Radioimmunoassay; D013045:Species Specificity; D013211:Staphylococcus aureus / Q000502:physiology*
chemical_list: D007375:Interleukin-1; D008070:Lipopolysaccharides; D012333:RNA, Messenger