Detection of rabies virus mRNA in mouse brain by using in situ hybridization with digoxigenin-labelled RNA probes.
A non-isotopic method of in situ hybridization (ISH) was developed for the detection of rabies virus RNA in paraffin-embedded tissues. Digoxigenin-labelled RNA probes for rabies virus glycoprotein mRNA were used. The method had good sensitivity and low backgrounds, and there was excellent cellular localization of signals. ISH wih digoxigenin-labelled probes was compared with ISH with 3H-labelled probes. This non-isotopic method of ISH is more convenient than the radiolabelled method, and it is quicker because a long autoradiographic exposure is not required.
Jackson
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Department of Medicine, Queen's University, Kingston, Ontario, Canada.
D000818:Animals; D001921:Brain / Q000382:microbiology*; D003856:Deoxyuracil Nucleotides; D004076:Digoxigenin / Q000031:analogs & derivatives; D005260:Female; D006023:Glycoproteins / Q000235:genetics; D051379:Mice; D008813:Mice, Inbred ICR; D015347:RNA Probes; D012333:RNA, Messenger / Q000302:isolation & purification; D012367:RNA, Viral / Q000302:isolation & purification*; D011818:Rabies / Q000382:microbiology; D011820:Rabies virus / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D014316:Tritium
D003856:Deoxyuracil Nucleotides; D006023:Glycoproteins; D015347:RNA Probes; D012333:RNA, Messenger; D012367:RNA, Viral; C070202:digoxigenin-11-deoxyuridine triphosphate; D014316:Tritium; D004076:Digoxigenin