title: Cloning and sequence of a functionally active cDNA encoding the mouse ubiquitin-activating enzyme E1.
abstract: A cDNA encoding the ubiquitin-activating enzyme, E1, was isolated from the mouse mammary carcinoma cell line, FM3A, and shown to complement mutant mouse cells deficient in the enzyme. The 3495-bp cDNA encodes 1058 amino acids (aa), and shares extensive homology with the human E1 enzyme at both the nucleotide and aa sequence levels.
authors.affiliation: National Institute of Genetics, Mishima, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Imai
authors.forename: S
authors.initials: S
authors.lastname: Kaneda
authors.forename: Y
authors.initials: Y
authors.lastname: Nagai
authors.forename: T
authors.initials: T
authors.lastname: Seno
authors.forename: D
authors.initials: D
authors.lastname: Ayusawa
authors.forename: F
authors.initials: F
authors.lastname: Hanaoka
authors.forename: F
authors.initials: F
authors.lastname: Yamao
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D008025:Ligases / Q000737:chemistry / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D044764:Ubiquitin-Activating Enzymes; D044767:Ubiquitin-Protein Ligases
chemical_list: D004247:DNA; D044767:Ubiquitin-Protein Ligases; D008025:Ligases; D044764:Ubiquitin-Activating Enzymes