Characterization of a synthetic peptide impurity by fast-atom bombardment-tandem mass spectrometry and gas chromatography-mass spectrometry.
Fast-atom bombardment-tandem mass spectrometry (FAB-MS-MS) was used to characterize the structure of atosiban, a synthetic oxytocin antagonist, and one of its synthesis-related impurities. The nature of the structural modification in the impurity of interest, replacement of the proline residue by 5-aminovaleric acid, was determined directly from its product ion MS-MS spectrum. Confirmation of the identity of the impurity was accomplished with GC-MS.
Burinsky
D J
DJ
R. W. Johnson Pharmaceutical Research Institute, Raritan, NJ 08869-0602.
Dunphy
R
R
Oyler
A R
AR
Shaw
C J
CJ
Cotter
M L
ML
D000596:Amino Acids / Q000032:analysis; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004340:Drug Contamination; D008401:Gas Chromatography-Mass Spectrometry; D010455:Peptides / Q000032:analysis*; D016339:Spectrometry, Mass, Fast Atom Bombardment; D014668:Vasotocin / Q000031:analogs & derivatives* / Q000032:analysis
D000596:Amino Acids; D010455:Peptides; C047046:atosiban; D014668:Vasotocin