Construction and characterization of radiation hybrids for chromosome 9, and their use in mapping cosmid probes on the chromosome.
Radiation hybrids were produced from a monochromosomal microcell hybrid (PK87-9) which contains only human chromosome 9 with an inserted marker on 9p. Doses of radiation ranging from 1000 to 8000 rads were used to produce a series of hybrids with different size fragments of human chromosome 9. The inserted dominant selectable marker was used to select for hybrids that preferentially maintain fragments of 9p. A panel of 53 radiation hybrids were characterized for 17 chromosome 9 markers. In addition, 17 hybrids were analyzed by fluorescent in situ hybridization (FISH). Hybrids were produced with breaks on both 9p and 9q, many of which appear to contain a single fragment of human chromosome 9. These hybrid cell lines were used to regionally localize 31 cosmids isolated from a chromosome 9 cosmid library. Six cosmids were mapped to intervals on 9p, six cosmids mapped to the centromeric region of the chromosome, and 19 mapped to 9q.
Jackson
C L
CL
Department of Pathology, Rhode Island Hospital, Providence 02903.
Britt
D E
DE
Graw
S L
SL
Potts
A
A
Santoro
K
K
Buckler
A J
AJ
Housman
D E
DE
Mark
H F
HF
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002874:Chromosome Mapping; D002899:Chromosomes, Human, Pair 9; D003360:Cosmids; D006224:Cricetinae; D015342:DNA Probes; D005453:Fluorescence; D005819:Genetic Markers / Q000235:genetics; D015698:Genomic Library; D006801:Humans; D006822:Hybrid Cells / Q000528:radiation effects; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction
D015342:DNA Probes; D005819:Genetic Markers