Chromosome studies in stimulated lymphocytes of 50 patients with B-cell chronic lymphocytic leukemia.
The chromosome constitutions of stimulated lymphocytes from 50 B-cell chronic lymphocytic leukemia patients were studied using different stimulation systems, i.e., TPA alone or associated with different cytokines. Adequate metaphases were obtained in 44 subjects (88%). Among 20 patients with abnormal karyotypes (45.5%), 7 had trisomy 12. The most frequent structural abnormality was a 14q+ resulting from translocations including one t(11;14) and two t(14;17), while deletions on the long arms of chromosomes 6 and 13 constituted a second common alteration. The most important finding in this series was the recurrence of a t(18;22) observed in two cases.
Delhomme-Bachy
M
M
Département d'Hématologie, Hôpital Nord, St Priest en Jarez, France.
Bertheas
M F
MF
Vasselon
C
C
Calmard-Oriol
P
P
Jaubert
J
J
Rimokh
R
R
Brizard
C P
CP
Guyotat
D
D
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002478:Cells, Cultured; D002869:Chromosome Aberrations / Q000175:diagnosis; D025063:Chromosome Disorders; D005260:Female; D006801:Humans; D007621:Karyotyping; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000235:genetics*; D008213:Lymphocyte Activation / Q000235:genetics*; D008297:Male; D008875:Middle Aged