title: Biosynthesis of pyrrolnitrin. Incorporation of 13C, 15N double-labelled D- and L-tryptophan.
abstract: Experiments on the incorporation of D- and L-[alanine-3-13C,2-15N]tryptophan into the antibiotic pyrrolnitrin in Pseudomonas aureofaciens confirmed earlier conclusions about the conversion of L-tryptophan into pyrrolnitrin. They also demonstrated that a fraction of the D isomer is incorporated without breakage of the 15N-carbon bond, consistent with the operation of a second pathway from D-tryptophan to pyrrolnitrin. Cell-free experiments confirmed the conversion of 3-(o-aminophenyl)pyrrole into aminopyrrolnitrin but failed to detect enzymatic oxidation of the latter to pyrrolnitrin.
authors.affiliation: Department of Chemistry, University of Washington, Seattle 91895.
authors.forename: P
authors.initials: P
authors.lastname: Zhou
authors.forename: U
authors.initials: U
authors.lastname: Mocek
authors.forename: B
authors.initials: B
authors.lastname: Siesel
authors.forename: H G
authors.initials: HG
authors.lastname: Floss
mesh_terms: D000409:Alanine / Q000378:metabolism; D002247:Carbon Isotopes; D004151:Dipeptides / Q000378:metabolism; D009587:Nitrogen Isotopes; D011549:Pseudomonas / Q000737:chemistry / Q000378:metabolism*; D011764:Pyrrolnitrin / Q000096:biosynthesis*; D013237:Stereoisomerism; D014364:Tryptophan / Q000737:chemistry / Q000378:metabolism*
chemical_list: D002247:Carbon Isotopes; D004151:Dipeptides; D009587:Nitrogen Isotopes; D014364:Tryptophan; D011764:Pyrrolnitrin; D000409:Alanine