title: A study of the incidence of different fluorescent Pseudomonas species and biovars in the microflora of fresh and spoiled meat and fish, raw milk, cheese, soil and water.
abstract: Of 182 various foodstuffs and environmental samples examined, 86% had microflora containing fluorescent Pseudomonas in differing proportions. A computer-aided technique was used to identify most of the 445 fluorescent strains. Pseudomonas fluorescens biovar V-1 was most frequently isolated (24%); it either predominated or was present in all types of samples. Other strains, belonging to the other subgroups of biovar V (V-2, V-4, V-5, V-6 and V-7), together represented 14.3%. We also identified Ps. fluorescens biovars I-1 and I-2 (13.9%), II-1 and II-3 (3.6%), III-1 and III-2 (8.7%), IV-2 (0.7%); Ps. putida A and B (11%); Ps. lundensis (10.3%); group B3 (2%) and Ps. aeruginosa (0.7%). Unidentified strains accounted for 10.6% of the flora, many resembling Ps. fluorescens biovar V. Although the presence of Ps. fluorescens V-1 was often marked, other taxa predominated or were present in large quantities in some particular samples, such as Ps. fluorescens I-1 in raw milk and cheese, Ps. lundensis in spoiled meat and Ps. fluorescens III-1 in spoiled fish. Pseudomonas putida A and B were evident in environmental rather than in food samples.
authors.affiliation: Istituto di Ispezione degli Alimenti di Origine Animale, Facoltà di Medicina Veterinaria, Università degli Studi di Milano, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Gennari
authors.forename: F
authors.initials: F
authors.lastname: Dragotto
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques; D002611:Cheese / Q000382:microbiology; D005399:Fishes / Q000382:microbiology; D005516:Food Microbiology; D008460:Meat / Q000382:microbiology; D008892:Milk / Q000382:microbiology; D011549:Pseudomonas / Q000145:classification / Q000302:isolation & purification*; D011550:Pseudomonas aeruginosa / Q000302:isolation & purification; D011551:Pseudomonas fluorescens / Q000302:isolation & purification; D012988:Soil Microbiology; D014871:Water Microbiology