The characteristic negative Cotton effect of ganglioside lactones observed by circular dichroism spectrometry.
Circular dichroism spectrometry was applied to gangliosides and their lactones and revealed that the lactones have a characteristic strong negative Cotton effect around 235 nm. Four monolactones and two dilactones, which were formed from GM4, GD3, and GD1b, gave molar ellipticities at the wavelength in magnitude of 10(4), while their parent gangliosides, along with other gangliosides such as GM3, GM1, GD1a, GT1b, and GQ1b, showed no distinct feature. Two ganglioside esters, GM4-methyl ester and O-Ac-GT1b did not show the Cotton effect. The molar ellipticities had an additivity with respect to the number of lactone rings. The Cotton effect was attributed to the carbonyl group on the lactone ring.
Terabayashi
T
T
Department of Chemistry, Kitasato University, Kanagawa, Japan.
Tsuda
M
M
Kawanishi
Y
Y
D000818:Animals; D001923:Brain Chemistry; D002240:Carbohydrate Sequence; D002942:Circular Dichroism; D005732:Gangliosides / Q000032:analysis* / Q000737:chemistry; D007783:Lactones / Q000032:analysis / Q000737:chemistry; D008969:Molecular Sequence Data; D015394:Molecular Structure; D014907:Whales
D005732:Gangliosides; D007783:Lactones