Immunosuppression induced by non-reducing acylated monosaccharide subunits of lipid A.
Synthetic acylated glucosamine monosaccharides, representative of the non-reducing subunit of lipid A, were compared for their ability to induce non-specific suppression of antibody forming cells. Five of nine analogs were found to be functional in this respect, indicating that these compounds, carrying a phosphate at C4 and acyl substituents at C2 and C3 are the smallest synthetic analogs of lipid A capable of eliciting non-specific immunosuppression. A comparison of the analogs inducing suppression with those testing negative revealed that (i) a single 3-hydroxymyristoyl group at C2 is common to 4/5 analogs inducing suppression; (ii) addition of an oxytetradecanoyl group to the 3-hyroxymyristoyl group at either C2 or C3 negated suppression; and (iii) extreme specificity was exhibited in suppression induction such that substitution of either lauric (C12) or palmitic (C16) for myristic acid (C14) at C2 voided transmission of the suppressive signal.
Odean
M J
MJ
Department of Microbiology/Immunology, University of Minnesota, Duluth 55812-2487.
Johnson
A G
AG
Mohrman
M
M
Hasegawa
A
A
Kiso
M
M
D000818:Animals; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D008050:Lipid A / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D013329:Structure-Activity Relationship
D007166:Immunosuppressive Agents; D008050:Lipid A