title: Tellurite-induced damage of the erythrocyte membrane. Manifestations and mechanisms.
abstract: Chemical and biophysical mechanisms underlying the thiol-dependent lytic action of tellurite (and selenite) on human erythrocytes were investigated using native and GSH-depleted cells. Exposure of GSH-depleted cells to tellurite alone produces oxidative cross-linking of membrane thiols paralleled by a moderate membrane leakiness comparable in its extent to that induced by other SH-oxidizing agents (diamide, periodate). Exposure to tellurite in presence of endogenous or exogenous GSH produces marked leakiness which stems from the formation of aqueous leaks permeant to ions and nonelectrolytes and sensitive to inhibition by phloretin. Apparent pore radii, derived from exclusion limits for polar non-electrolytes, range from 0.3 to at least 1.3 nm. Leak size increases with increasing exposure time and concentration of the modifier. Leak formation is paralleled by membrane rigidification based on the cross-linking of spectrin. Thiol-dependent leak formation by tellurite in GSH-depleted cells can be sustained not only by exogenous GSH but also by other thiols. Progress of leak formation by tellurite/thiol can not be reliably quenched by procedures such as removal of tellurite from the medium, inhibition of anion transport via band-3 protein, washing of the cells or low temperature. The reaction can, however, be terminated, even in the presence of tellurite, by addition of N-ethylmaleimide, presumably due to the blockage of thiols or thiol-analogous tellurium compounds. N-ethylmaleimide even brings about a partial reversal of leakiness, suggesting the contribution of a reversible and an irreversible component of tellurite damage. Membrane perturbation by tellurite/thiol involves the formation of a membrane permeant tellurium species, possibly HTe-, which is likely to induce progressive damage of membrane proteins by a redox shuttle going along with a formation of elemental tellurium and its reduction by thiols.
authors.affiliation: Institut für Physiologie, Medizinische Fakultät, RWTH Aachen, Germany.
authors.forename: B
authors.initials: B
authors.lastname: Deuticke
authors.forename: P
authors.initials: P
authors.lastname: Lütkemeier
authors.forename: B
authors.initials: B
authors.lastname: Poser
mesh_terms: D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid / Q000031:analogs & derivatives; D002463:Cell Membrane Permeability / Q000187:drug effects*; D004137:Dinitrochlorobenzene; D004305:Dose-Response Relationship, Drug; D004896:Erythritol / Q000032:analysis; D004910:Erythrocyte Membrane / Q000187:drug effects* / Q000473:pathology; D004912:Erythrocytes / Q000187:drug effects*; D005033:Ethylmaleimide; D005978:Glutathione / Q000032:analysis; D006801:Humans; D008560:Membrane Fluidity / Q000187:drug effects; D008563:Membrane Lipids / Q000737:chemistry; D008565:Membrane Proteins / Q000737:chemistry; D010084:Oxidation-Reduction; D013438:Sulfhydryl Compounds / Q000494:pharmacology; D013691:Tellurium / Q000037:antagonists & inhibitors / Q000494:pharmacology*
chemical_list: D004137:Dinitrochlorobenzene; D008563:Membrane Lipids; D008565:Membrane Proteins; D013438:Sulfhydryl Compounds; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid; C026899:potassium tellurate(IV); D005978:Glutathione; D013691:Tellurium; D005033:Ethylmaleimide; D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; D004896:Erythritol