[Does ionizing radiation induce an adaptive response in mouse L5178Y lymphoma cells?].
Growth kinetics of LY-S and LY-R cells (radiosensitive and radioresistant sublines of murine lymphoma L5178Y) has been investigated after beta-irradiation at cumulative doses of 1.5 to 20 cGy and dose rates of 0.8-10 mGy/h. It has been found that after 48 h culture in a complete medium the number of cells differed 5 times, whereas after X- and gamma-irradiation, Do values differed 1.62 times. Using the growth rate as the end point in evaluating the combined effect of beta-irradiation (10 cGy) and subsequent X-irradiation with lethal doses, we observed an increased relative cell number, in comparison to that after X-irradiation alone (an "adaptive response", using this criterion), in LY-S cells irradiated with a dose of 2 Gy. In contrast, when reproductive death of LY-S and LY-R cells the end-point analyzed, the lethal effect of consecutive beta- and X-irradiation in LY-S cells was higher than that expected for X-radiation alone (the synergistic effect).
Afanas'ev
G G
GG
Zagurski
S
S
Burachevska
I
I
Shumel'
I
I
D000818:Animals; D001610:Beta Particles; D002452:Cell Count; D016923:Cell Death; D003470:Culture Media; D006801:Humans; D066298:In Vitro Techniques; D007940:Leukemia L5178 / Q000473:pathology / Q000532:radiotherapy*; D051379:Mice; D011839:Radiation, Ionizing; D011879:Radiotherapy Dosage; D013997:Time Factors
D003470:Culture Media
Indutsiruet li ioniziruiushchee izluchenie adaptivnyĭ otvet v kletkakh limfomy L5178Y mysheĭ?