Cloning of a Bacteroides fragilis chromosomal determinant coding for 5-nitroimidazole resistance.
Strain BF8 is a plasmid-free Bacteroides fragilis, resistant to 5-nitroimidazole (5-Ni) antibiotics (metronidazole, ornidazole and tinidazole). The resistance was transferable by conjugation into Bacteroides fragilis BF638R. The total DNA of a Nir transconjugant was used for the construction of a Sau3A genomic library in a B. fragilis cloning vector pFK707 delta H1 (4.2 kb). By electrotransformation of strain BF638R, a recombinant plasmid containing an insert of 5.4 kb was obtained which conferred to the host strain the resistance to 5-Ni. The physical map of the insert was established. After deletion analysis of the insert, the Nir determinant was localized on a HpaII-HincII fragment of 1.6 kb in size. This Nir determinant has been compared by Southern-blot analysis with other Bacteroides Nir determinants of plasmid origin.
Haggoud
A
A
Unité des Anaérobies, Institut Pasteur, Paris, France.
Reysset
G
G
Sebald
M
M
D001441:Bacteroides fragilis / Q000187:drug effects / Q000235:genetics*; D015139:Blotting, Southern; D003001:Cloning, Molecular; D003227:Conjugation, Genetic; D004352:Drug Resistance, Microbial / Q000235:genetics; D008795:Metronidazole / Q000494:pharmacology; D009593:Nitroimidazoles / Q000494:pharmacology*; D009950:Ornidazole / Q000494:pharmacology; D010957:Plasmids; D014011:Tinidazole / Q000494:pharmacology; D014169:Transformation, Bacterial
D009593:Nitroimidazoles; D014011:Tinidazole; D008795:Metronidazole; D009950:Ornidazole; C052587:4-nitroimidazole