Isolation and characterization of new peptide antibiotics, plusbacins A1-A4 and B1-B4.
New antibiotics, plusbacins A1-A4 and B1-B4, were isolated from the culture broth of a strain of Pseudomonas sp. These antibiotics were isolated as a complex by column chromatographies on Diaion HP-20 and silica gel, and then separated by HPLC. They are amphoteric in nature. The hydrochlorides are obtained as colorless powders, soluble in methanol and alkaline water. From their physico-chemical properties, these antibiotics are presumed to be acyloctapeptides containing a lactone linkage, and their differences occur in amino acid and fatty acid residues. The antibiotics are active against Gram-positive bacteria in vitro and in vivo.
Shoji
J
J
Shionogi Research Laboratories, Shionogi & Co., Ltd., Osaka, Japan.
Hinoo
H
H
Katayama
T
T
Matsumoto
K
K
Tanimoto
T
T
Hattori
T
T
Higashiyama
I
I
Miwa
H
H
Motokawa
K
K
Yoshida
T
T
D000596:Amino Acids / Q000032:analysis; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D005227:Fatty Acids / Q000032:analysis; D005285:Fermentation; D010456:Peptides, Cyclic / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D011549:Pseudomonas / Q000145:classification / Q000378:metabolism*
D000596:Amino Acids; D000900:Anti-Bacterial Agents; D005227:Fatty Acids; D010456:Peptides, Cyclic