[Effects of UFT and loxiglumide (CR1505) on liver metastasis of human pancreatic cancer cell line, KP-1 N in nude mice].
Effects of caerulein (CCK), 5-FU and CCK antagonist, loxiglumide (CR1505), were studied on the growth of human pancreatic cancer cell line, KP-1 N, in vitro. And effects of UFT and CR 1505 were also studied on liver metastasis in nude mice. The growth of KP-1 N was stimulated approximately 40% by addition of 10(-10) M of CCK in vitro. CR1505 antagonized the action of CCK, that is, the 40% growth rate increase was suppressed by addition of 25 microM of CR1505. Moreover, the growth rate of the cells dose-dependently decreased by the addition of CR1505. 5-FU also dose-dependently inhibited the growth of KP-1 N in culture. 5-FU additionally decreased the growth rate of KP-1 N in combination with CR1505. A number of metastatic nodules were found in the liver of nude mice a month after injections of KP-1 N cells into the spleen CR1505 suppressed the liver metastasis in nude mice which were administered with UFT. These results suggest that CR1505 would be useful for the treatment of human pancreatic cancer in combination with UFT.
Shimazoe
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T
Chemotherapy Division of Research Institute, National Kyushu Cancer Center, Fukuoka, Japan.
Funakoshi
A
A
Kono
A
A
D000818:Animals; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002766:Cholecystokinin / Q000037:antagonists & inhibitors*; D006801:Humans; D008113:Liver Neoplasms / Q000473:pathology / Q000556:secondary*; D051379:Mice; D008819:Mice, Nude; D010190:Pancreatic Neoplasms / Q000473:pathology*; D011377:Proglumide / Q000031:analogs & derivatives* / Q000494:pharmacology; D005641:Tegafur / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000187:drug effects; D014498:Uracil / Q000494:pharmacology
D005641:Tegafur; D014498:Uracil; C053737:loxiglumide; D002766:Cholecystokinin; D011377:Proglumide