title: Plasmid pLTRpoly: a versatile high-efficiency mammalian expression vector.
abstract: Plasmid pLTRpoly is an expression vector enabling high-level expression of introduced genes in a variety of cell types. A large multiple cloning site (MCS) and the availability of the full-length nucleotide sequence facilitate the generation of constructs using this vector. Here, constructs made with pLTRpoly have been tested in NIH3T3 mouse fibroblasts.
authors.affiliation: Department of Virology and Pathology, University of Helsinki, Finland.
authors.forename: T P
authors.initials: TP
authors.lastname: Mäkelä
authors.forename: J
authors.initials: J
authors.lastname: Partanen
authors.forename: M
authors.initials: M
authors.lastname: Schwab
authors.forename: K
authors.initials: K
authors.lastname: Alitalo
mesh_terms: D016475:3T3 Cells; D000818:Animals; D015152:Blotting, Northern; D003001:Cloning, Molecular; D005822:Genetic Vectors / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D008979:Moloney murine leukemia virus / Q000235:genetics*; D010957:Plasmids / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*; D015183:Restriction Mapping