title: Increased phosphorylation of nuclear substrates for rat brain protein kinase C in regenerating rat liver nuclei.
abstract: Protein phosphorylation catalysed by rat brain protein kinase C (PKC) has been studied in nuclei isolated from normal and regenerating rat liver. Histone H1 and a 40,000 molecular weight protein were hyperphosphorylated at all the explored regeneration times, ranging from 3 to 22 h after partial hepatectomy. Phosphorylation of the two substrates was totally dependent on calcium and lipids and was abolished by low concentration of staurosporine. The observed early change of phosphate content of histone H1 and of the 40,000 molecular weight protein on the time scale of liver regeneration suggests that PKC might be involved in the initial nuclear events leading to cell proliferation.
authors.affiliation: Istituto di Anatomia Umana Normale, Università di Ferrara, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Mazzoni
authors.forename: C
authors.initials: C
authors.lastname: Carini
authors.forename: A
authors.initials: A
authors.lastname: Matteucci
authors.forename: A M
authors.initials: AM
authors.lastname: Martelli
authors.forename: V
authors.initials: V
authors.lastname: Bertagnolo
authors.forename: M
authors.initials: M
authors.lastname: Previati
authors.forename: R
authors.initials: R
authors.lastname: Rana
authors.forename: A
authors.initials: A
authors.lastname: Cataldi
authors.forename: S
authors.initials: S
authors.lastname: Capitani
mesh_terms: D000818:Animals; D001921:Brain / Q000201:enzymology*; D002467:Cell Nucleus / Q000378:metabolism*; D008099:Liver / Q000201:enzymology* / Q000378:metabolism; D008115:Liver Regeneration; D008297:Male; D008970:Molecular Weight; D010766:Phosphorylation; D011493:Protein Kinase C / Q000378:metabolism*; D011506:Proteins / Q000032:analysis / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D011506:Proteins; D011493:Protein Kinase C