Human perforin (PRF1) maps to 10q22, a region that is syntenic with mouse chromosome 10.
Perforin (PRF1) is a cytolytic, channel-forming protein of cytolytic T cells, natural killer cells, and granulated metrial gland cells and plays a crucial role in the killer cell-mediated elimination of virally infected host cells, tumor cells, and allotransplants. Two-thirds of the perforin sequence is homologous to the lytic, channel-forming complement proteins C6, C7, C8 alpha, C8 beta, and C9. Using cosmid DNA containing the PRF1 gene as a probe for fluorescence in situ hybridization, we have reevaluated its chromosomal location. Previously assigned to chromosome 17q11-q21, it has now been mapped to 10q22. The human PRF1 locus lies within a conserved synteny segment present on mouse chromosome 10, consistent with the previous chromosomal assignment of mouse perforin. The perforin locus is not linked to any of the genes of the terminal complement system.
Fink
T M
TM
Deutsches Krebsforschungszentrum, Schwerpunkt Angewandte Tumorvirologie, Heidelberg, Germany.
Zimmer
M
M
Weitz
S
S
Tschopp
J
J
Jenne
D E
DE
Lichter
P
P
D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D002875:Chromosomes; D002879:Chromosomes, Human, Pair 10; D005453:Fluorescence; D006801:Humans; D007621:Karyotyping; D008562:Membrane Glycoproteins; D008565:Membrane Proteins / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D054353:Perforin; D052899:Pore Forming Cytotoxic Proteins; D013602:T-Lymphocytes, Cytotoxic / Q000378:metabolism*
D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D052899:Pore Forming Cytotoxic Proteins; D054353:Perforin