Microplate and dot immunoassays for the serodiagnosis of tuberculosis.
A simple dot enzyme immunoassay based on the recognition of serum IgG antibody to a 30,000 dalton native antigen purified from culture filtrates of Mycobacterium tuberculosis was developed and compared with a standard plate enzyme-linked immunosorbent assay in the serodiagnosis of tuberculosis. The previously described favorable test characteristics of plate enzyme-linked immunoassay were confirmed; although the dot enzyme immunoassay was promising, it was less satisfactory. Dot enzyme immunoassay may have its most promising use as a screening test for situations of limited technical facilities. Both plate enzyme-linked immunoassay and dot enzyme immunoassay had markedly reduced sensitivities in persons with human immunodeficiency virus infection.
McDonough
J A
JA
Department of Medicine, Case Western Reserve University, Cleveland, OH.
Sada D
E
E
Sippola
A A
AA
Ferguson
L E
LE
Daniel
T M
TM
D000942:Antigens, Bacterial / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D015658:HIV Infections / Q000150:complications*; D006679:HIV Seropositivity; D006801:Humans; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000032:analysis / Q000097:blood*; D009169:Mycobacterium tuberculosis / Q000276:immunology; D015995:Prevalence; D012016:Reference Values; D012698:Serologic Tests / Q000379:methods; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis* / Q000453:epidemiology / Q000209:etiology
D000942:Antigens, Bacterial; D007074:Immunoglobulin G