title: Determination of the coumarin derivative cloricromene acid in rabbit plasma and platelets.
abstract: Two methods for the determination of cloricromene acid in biological samples are described. Cloricromene acid is a catabolite of cloricromene, a coumarin derivative which is active in the cardiovascular system. After oral administration of cloricromene to a rabbit, plasma and platelets were taken at different times and cloricromene acid was then isolated by solid-phase extraction with Sep-Pak C18 cartridges using acetonitrile-tetrahydrofuran-20% aqueous acetic acid (15:11:74, v/v/v) as eluent. The analyses were performed by reversed-phase high-performance liquid chromatography (RP-HPLC) combined with fluorescence detection with excitation at 310 nm and emission at 390 nm. The limit of quantification by RP-HPLC was about 50 pg. The catabolite in the plasma was identified by continuous-flow fast atom bombardment mass spectrometry (CF-FAB-MS), also used as a complementary means of RP-HPLC determination. The results obtained by RP-HPLC and CF-FAB-MS showed good agreement.
authors.affiliation: Department of Pharmacokinetics, Fidia Research Laboratories, Abano Terme, Italy.
authors.forename: R
authors.initials: R
authors.lastname: Mariot
authors.forename: L
authors.initials: L
authors.lastname: Zangirolami
authors.forename: P
authors.initials: P
authors.lastname: Bellato
authors.forename: S
authors.initials: S
authors.lastname: Chen
authors.forename: G
authors.initials: G
authors.lastname: Pieraccini
authors.forename: G
authors.initials: G
authors.lastname: Moneti
mesh_terms: D000818:Animals; D001792:Blood Platelets / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D002866:Chromonar / Q000031:analogs & derivatives* / Q000097:blood; D007202:Indicators and Reagents; D008297:Male; D011817:Rabbits; D016339:Spectrometry, Mass, Fast Atom Bombardment
chemical_list: D007202:Indicators and Reagents; C025945:cloricromen; D002866:Chromonar