title: Structure of the human DNA ligase I gene.
abstract: The gene encoding DNA ligase I, the major DNA ligase activity in proliferating mammalian cells, maps to human chromosome 19q13.2-13.3. We have determined the complete structure of the gene, which is composed of 28 exons spanning 53kb on this chromosome. The first exon is untranslated, and utilises a GC dinucleotide instead of the canonical GT splice donor. The 5' flanking region lacks a TATA box and is highly GC-rich, as is characteristic of a 'housekeeping' gene. In common with the promoters of genes encoding other DNA replication enzymes, such as DNA polymerase alpha, the 5' flanking region of the DNA ligase I gene contains recognition elements for several transcription factors which may mediate increased expression in quiescent cells in response to growth factors.
authors.affiliation: Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, Hertfordshire, UK.
authors.forename: P
authors.initials: P
authors.lastname: Noguiez
authors.forename: D E
authors.initials: DE
authors.lastname: Barnes
authors.forename: H W
authors.initials: HW
authors.lastname: Mohrenweiser
authors.forename: T
authors.initials: T
authors.lastname: Lindahl
mesh_terms: D001483:Base Sequence; D015139:Blotting, Southern; D002888:Chromosomes, Human, Pair 19; D003001:Cloning, Molecular; D003360:Cosmids / Q000235:genetics; D000072481:DNA Ligase ATP; D011088:DNA Ligases / Q000235:genetics*; D005091:Exons / Q000235:genetics; D015723:Gene Library; D006801:Humans; D007438:Introns / Q000235:genetics; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D014407:Tumor Cells, Cultured
chemical_list: C000607892:LIG1 protein, human; D011088:DNA Ligases; D000072481:DNA Ligase ATP