title: Synthesis of the proline analogue [2,3-3H]azetidine-2-carboxylic acid. Uptake and incorporation in Arabidopsis thaliana and Escherichia coli.
abstract: Azetidine-2-carboxylic acid, the 4-membered ring noranalogue of proline, is regularly used in the study of proline metabolism as well as the study of protein conformation. We prepared D,L-[2,3-3H]azetidine-2-carboxylic acid with an optimized 10% yield from commercially available 4-amino-[2,3-3H]butyric acid. Purification was performed by fast-protein liquid chromatography. The biological activity was checked in both Arabidopsis thaliana and Escherichia coli. The obtained specific activity of 10 mCi/mmol was sufficient for most uptake and incorporation studies.
authors.affiliation: Laboratorium voor Genetica, Universiteit Gent, Belgium.
authors.forename: N
authors.initials: N
authors.lastname: Verbruggen
authors.forename: M
authors.initials: M
authors.lastname: van Montagu
authors.forename: E
authors.initials: E
authors.lastname: Messens
mesh_terms: D001383:Azetidinecarboxylic Acid / Q000138:chemical synthesis* / Q000378:metabolism*; D004926:Escherichia coli / Q000378:metabolism*; D007553:Isotope Labeling; D010944:Plants / Q000378:metabolism*; D014316:Tritium
chemical_list: D014316:Tritium; D001383:Azetidinecarboxylic Acid