Alginate coating of islets of Langerhans: in vitro studies on a new method for microencapsulation for immuno-isolated transplantation.
Immuno-isolated transplantation offers the attractive prospect of being able to transplant xenogeneic islets without immunosuppression. This study introduces a completely new method of coating single islets using a homogeneous alginate membrane approximately 10 microns thick. During glucose challenge (perifusion and static incubation) encapsulated islets show the same pattern and quantity of insulin release as non-encapsulated controls. This encapsulation method markedly reduces the amount of transplanted material by reducing the size of the capsule. It is suggested that encapsulated islets may be transplanted into sites such as the renal capsule or omentum or even by intraportal injection into the liver.
Zekorn
T
T
Medizinische Klinik III und Poliklinik, Justus Liebig-Universität, Giessen, Federal Republic of Germany.
Siebers
U
U
Horcher
A
A
Schnettler
R
R
Zimmermann
U
U
Bretzel
R G
RG
Federlin
K
K
D000464:Alginates; D000818:Animals; D002453:Cell Cycle; D002478:Cells, Cultured; D004339:Drug Compounding / Q000379:methods; D005947:Glucose / Q000494:pharmacology; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D007165:Immunosuppression Therapy; D007328:Insulin / Q000378:metabolism; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D016381:Islets of Langerhans Transplantation / Q000379:methods*; D008297:Male; D008938:Mitosis; D051381:Rats; D011917:Rats, Inbred Lew
D000464:Alginates; D006603:Hexuronic Acids; D007328:Insulin; D020723:Glucuronic Acid; D005947:Glucose