title: Platelet-derived growth factor B-chain-like immunoreactivity in the developing and adult rat brain.
abstract: Platelet-derived growth factors (PDGFs) are growth-regulatory molecules that stimulate chemotaxis, proliferation and increased metabolism, primarily of connective tissue cells. In our previous paper, we have demonstrated the ubiquitous localization of PDGF B-chain-containing proteins in neurons and expression of transcripts for PDGF A-chain, B-chain and the two forms of the PDGF receptor in the brains of non-human primates. In the present study, the cellular localization of PDGF B-chain in developing and adult rat brains was analyzed using immunocytochemistry with a PDGF B-chain-specific monoclonal antibody. Intense PDGF B-chain immunoreactivity (PDGFB-I) was distributed around the continuously regenerating primary olfactory neurons at all stages of development from embryo to adult. The major part of PDGFB-I associated with neurons appeared some time after birth and increased with age. PDGFB-I appeared in several nerve fiber systems during earlier stages of development and gradually decreased with age. In conjunction with other data showing the existence of functional PDGF receptor beta-subunits in the neurons, these data suggest a possible role for PDGF B-chain as a neurotrophic or neuroregulatory factor in both developing and mature brains.
authors.affiliation: Department of Pathology, University of Washington, Seattle 98195.
authors.forename: A
authors.initials: A
authors.lastname: Sasahara
authors.forename: J N
authors.initials: JN
authors.lastname: Kott
authors.forename: M
authors.initials: M
authors.lastname: Sasahara
authors.forename: E W
authors.initials: EW
authors.lastname: Raines
authors.forename: R
authors.initials: R
authors.lastname: Ross
authors.forename: L E
authors.initials: LE
authors.lastname: Westrum
mesh_terms: D000818:Animals; D001921:Brain / Q000254:growth & development; D001923:Brain Chemistry / Q000502:physiology*; D002531:Cerebellum / Q000737:chemistry / Q000254:growth & development; D006624:Hippocampus / Q000737:chemistry / Q000254:growth & development; D007124:Immunoenzyme Techniques; D009832:Olfactory Nerve / Q000737:chemistry / Q000254:growth & development; D009900:Optic Nerve / Q000737:chemistry / Q000254:growth & development; D010982:Platelet-Derived Growth Factor / Q000032:analysis* / Q000502:physiology; D011518:Proto-Oncogene Proteins / Q000032:analysis* / Q000502:physiology; D020574:Proto-Oncogene Proteins c-sis; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D010982:Platelet-Derived Growth Factor; D011518:Proto-Oncogene Proteins; D020574:Proto-Oncogene Proteins c-sis