Demonstration of artifactual coupling between spinal neurons and glial cells during intracellular recording with micropipette electrodes.
Intracellular recordings with electrophysiological properties that are characteristic of both neurons and glial cells were obtained in the isolated spinal dorsal horn. Intracellular staining supports an idea that these elements may become artifactually coupled via the recording electrode. This coupling can be a gradual process, occurring over periods up to 30 min, and may have implications for the interpretation of slow, afferent-evoked potentials in some dorsal horn neurons.
Schneider
S P
SP
Department of Physiology, University of North Carolina, Chapel Hill 27599-7545.
D000818:Animals; D016477:Artifacts; D002450:Cell Communication; D004566:Electrodes; D004594:Electrophysiology; D006735:Horseradish Peroxidase; D007425:Intracellular Membranes / Q000502:physiology; D009457:Neuroglia / Q000502:physiology*; D009474:Neurons / Q000502:physiology*; D013116:Spinal Cord / Q000166:cytology*
D006735:Horseradish Peroxidase