title: Reversed-phase ion-pair high-performance liquid chromatographic determination of triclabendazole metabolites in serum and urine.
abstract: An ion-pair high-performance liquid chromatographic method was developed for measuring the concentrations of triclabendazole metabolites (sulphoxide and sulphone) in plasma and urine samples. The diluted biological fluids are ultrafiltered before chromatography through a 30,000 relative molecular mass cut-off filter and then injected into a C18 column. They are then isocratically eluted with a mobile phase consisting of 0.05 M phosphate buffer (pH 7.0)-acetonitrile (55:45, v/v) with addition of 1.0 mmol/l sodium decanesulphonate and monitored by ultraviolet-visible spectrophotometry at 312 nm. Recoveries over the range 0.01-9.0 micrograms/ml for triclabendazole sulphoxide and sulphone are, respectively, 91.7% and 91.6% in serum and 90.3% and 90.2% in urine. For both metabolites, the limit of detection is 10 ng/ml in both urine and serum.
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Universidad de León, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Negro
authors.forename: M L
authors.initials: ML
authors.lastname: Alvarez-Bujidos
authors.forename: A I
authors.initials: AI
authors.lastname: Ortiz
authors.forename: J C
authors.initials: JC
authors.lastname: Cubría
authors.forename: R
authors.initials: R
authors.lastname: Méndez
authors.forename: D
authors.initials: D
authors.lastname: Ordóñez
mesh_terms: D000818:Animals; D001562:Benzimidazoles / Q000032:analysis* / Q000097:blood / Q000652:urine; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002851:Chromatography, High Pressure Liquid; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D011817:Rabbits; D000077682:Triclabendazole; D014462:Ultrafiltration
chemical_list: D001562:Benzimidazoles; D007202:Indicators and Reagents; D000077682:Triclabendazole