Radioprotection of spleen by a combination of 5-hydroxy-L-tryptophan and 2-aminoethylisothiuronium bromide hydrobromide in lethally irradiated rats.
Radioprotective efficiency of the combination of 5-hydroxy-L-tryptophan (5-HTP, 100 mg/kg) with 2-aminoethylisothiuronium bromide hydrobromide (AET, 20 mg/kg) in protecting splenic tissue following 8 Gy whole-body gamma radiation (WBGR) has been studied in adult male Sprague Dawley rats. Loss in splenic weight, splenic cells and DNA was observed following & Gy WBGR, which was modified significantly in 5-HTP+AET (ip) pretreated group. The study further showed that pre-treatment with this formulation led to an increased superoxide dismutase (SOD) activity and a decrease in lactate/pyruvate ratio in the cytosol fraction of the spleen.
Basu
S K
SK
Radiation Biology Division, Institute of Nuclear Medicine and Allied Sciences, Delhi, India.
Srinivasan
M N
MN
Chuttani
K
K
George
S
S
D006916:5-Hydroxytryptophan / Q000008:administration & dosage*; D000818:Animals; D004247:DNA / Q000187:drug effects / Q000528:radiation effects; D004353:Drug Evaluation, Preclinical; D004359:Drug Therapy, Combination; D008297:Male; D011833:Radiation Injuries, Experimental / Q000378:metabolism / Q000517:prevention & control*; D011837:Radiation-Protective Agents / Q000008:administration & dosage*; D051381:Rats; D011919:Rats, Inbred Strains; D013154:Spleen / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects; D013482:Superoxide Dismutase / Q000187:drug effects / Q000528:radiation effects; D013997:Time Factors; D000338:beta-Aminoethyl Isothiourea / Q000008:administration & dosage*
D011837:Radiation-Protective Agents; D000338:beta-Aminoethyl Isothiourea; D004247:DNA; D006916:5-Hydroxytryptophan; D013482:Superoxide Dismutase