A convenient method for regional monoamine oxidase-A determination by [14C]clorgyline autoradiography.
The availability of clorgyline for regional monoamine oxidase-A (MAO-A) determination was examined using [14C]clorgyline in rat. [14C]Clorgyline was synthesized by the methylation reaction of N-desmethyl-clorgyline and [14C]methyliodide in dimethylformamide with high radiochemical yield. The MAO-A distribution map by autoradiography correlated with that by histochemical technique and its quantity was consistent with the calculated MAO-A amount based on previous reports. The combination of labeled clorgyline and autoradiographic technique will promise the quantitative measurement of regional MAO-A distribution.
Murakami
M
M
Department of Radiology, Research Institute for Brain and Blood Vessels-Akita, Japan.
Kondoh
Y
Y
Weimin
Y
Y
Mizusawa
S
S
Nakamichi
H
H
Takahashi
K
K
Sasaki
H
H
Iida
H
H
Miura
S
S
Kanno
I
I
D000818:Animals; D001345:Autoradiography / Q000379:methods*; D001921:Brain / Q000201:enzymology; D002250:Carbon Radioisotopes; D003010:Clorgyline / Q000138:chemical synthesis; D008995:Monoamine Oxidase / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D014055:Tomography, Emission-Computed
D002250:Carbon Radioisotopes; D008995:Monoamine Oxidase; D003010:Clorgyline