title: Amphibian oocytes and sphere organelles: are the U snRNA genes amplified?
abstract: The sphere organelles (spheres) of Xenopus and other amphibian oocytes are known to contain small nuclear ribonucleoprotein particles (snRNPs) and have been suggested to play a role in snRNP complex assembly. Coupled with the similarities that exist between spheres and nucleoli and the quantitative and kinetic aspects of snRNA synthesis in the Xenopus oocyte, we have investigated whether or not the U snRNA encoding genes are amplified in Xenopus oogenesis, the spheres being possible sites for the location of such extrachromosomal gene copies. By applying a number of quantitative nucleic acid hybridization procedures to both total and fractionated oocyte and somatic DNA, employing both homologous and heterologous U snRNA gene probes and suitable amplification and non-amplification control probes, we show that the U snRNA genes do not undergo any major amplification in Xenopus oogenesis. Therefore, the analogy between the sphere organelles and nucleoli appears to be limited. The role of the spheres and their relationship to other snRNP containing structures, specifically B snurposomes, and the sphere organizer loci remains obscure.
authors.affiliation: Research Institute of Molecular Pathology, Vienna, Austria.
authors.forename: S
authors.initials: S
authors.lastname: Phillips
authors.forename: M
authors.initials: M
authors.lastname: Cotten
authors.forename: F
authors.initials: F
authors.lastname: Laengle-Rouault
authors.forename: G
authors.initials: G
authors.lastname: Schaffner
authors.forename: M L
authors.initials: ML
authors.lastname: Birnstiel
mesh_terms: D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002499:Centrifugation, Density Gradient; D004247:DNA; D005784:Gene Amplification; D006801:Humans; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D009865:Oocytes / Q000378:metabolism* / Q000648:ultrastructure; D015388:Organelles / Q000378:metabolism*; D012342:RNA, Small Nuclear / Q000235:genetics*; D014981:Xenopus
chemical_list: D015345:Oligonucleotide Probes; D012342:RNA, Small Nuclear; D004247:DNA