title: Incorporation of the enantiomers of lipoic acid into the pyruvate dehydrogenase complex from Escherichia coli in vivo.
abstract: The uptake of 35S-labelled enantiomers of lipoic acid into cells from Escherichia coli was studied. The R-enantiomer was taken up by a factor of two more efficiently than the S-form. Autoradiography of polyacrylamide gels of partially purified pyruvate dehydrogenase complex from these cells showed that only the R-lipoic acid was covalently incorporated as a cofactor into the dihydrolipoamide acetyltransferase component of the pyruvate dehydrogenase complex.
authors.affiliation: Physiologisch-Chemisches Institut Universität Tübingen, Germany.
authors.forename: R
authors.initials: R
authors.lastname: Oehring
authors.forename: H
authors.initials: H
authors.lastname: Bisswanger
mesh_terms: D001345:Autoradiography; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000201:enzymology*; D011768:Pyruvate Dehydrogenase Complex / Q000737:chemistry / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism; D008063:Thioctic Acid / Q000032:analysis / Q000378:metabolism*
chemical_list: D011768:Pyruvate Dehydrogenase Complex; D008063:Thioctic Acid