Correlation between the induction of an immediate early gene, zif/268, and long-term potentiation in the dentate gyrus.
Expression of the immediate early gene zif/268 (also termed NGFI-A, Krox 24, TIS8 and Egr-1) was investigated in awake rats following various long-term potentiation (LTP) induction protocols. zif/268 mRNA (Northern blots) and protein (immunohistochemistry) levels sharply increased following LTP, and followed a time course characteristic of other immediate early genes. When measured across 3 tetanization protocols known to produce differing degrees of LTP persistence, zif/268 induction was found to be more highly correlated with LTP duration than with the magnitude of initial LTP. These data support the hypothesis that the immediate early gene zif/268 plays a role as a third messenger in the cascade of cellular and nuclear events that govern the persistence of LTP.
Richardson
C L
CL
Department of Biochemistry, University of Otago, Dunedin, New Zealand.
Tate
W P
WP
Mason
S E
SE
Lawlor
P A
PA
Dragunow
M
M
Abraham
W C
WC
D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D005071:Evoked Potentials / Q000502:physiology; D015870:Gene Expression / Q000502:physiology*; D006624:Hippocampus / Q000502:physiology*; D007150:Immunohistochemistry; D008297:Male; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics*; D015345:Oligonucleotide Probes; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors
D009419:Nerve Tissue Proteins; D015345:Oligonucleotide Probes; D012333:RNA, Messenger