Evidence for in vivo incorporation of D-lactate into peptidoglycan precursors of vancomycin-resistant enterococci.
The VanA ligase encoded by the vancomycin resistance plasmid pIP816 of Enterococcus faecium BM4147 condenses D-alanine with various D-2-hydroxy and D-2-amino acids in vitro. D-Lactate added to the culture medium restored the vancomycin resistance of a strain that does not produce the VanH dehydrogenase and therefore appears to be a substrate of VanA in vivo.
Arthur
M
M
Unité des Agents Antibactériens, Institut Pasteur, Paris, France.
Molinas
C
C
Bugg
T D
TD
Wright
G D
GD
Walsh
C T
CT
Courvalin
P
P
D004352:Drug Resistance, Microbial; D016984:Enterococcus faecium / Q000187:drug effects / Q000378:metabolism*; D007773:Lactates / Q000378:metabolism*; D019344:Lactic Acid; D008826:Microbial Sensitivity Tests; D010457:Peptidoglycan / Q000378:metabolism*; D014640:Vancomycin / Q000378:metabolism / Q000494:pharmacology
D007773:Lactates; D010457:Peptidoglycan; D019344:Lactic Acid; D014640:Vancomycin