title: Selective inhibition of glial cell metabolism in vivo by fluorocitrate.
abstract: The effect of fluorocitrate on glial and neuronal amino acid metabolism was studied. One nmol of fluorocitrate administered intrastriatally in the rat caused a 95% reduction of glutamine formation from [14C]acetate, a substrate which enters the glial cells selectively. The metabolism of [14C]glucose which enters neurons, was unaffected by fluorocitrate treatment except for the glutamine formation. This is evidence that fluorocitrate is a selective inhibitor of the glial Krebs' cycle. [14C]Citrate and 2-oxoglutarate labelled amino acids in a manner similar to [14C]acetate, which shows that these substrates are taken up and metabolized by glial cells. Differences in the labelling of gamma-aminobutyric acid (GABA) from [14C]acetate and citrate suggest that astrocytes associated with GABAergic and glutamatergic nerve terminals may differ in their preference for amino acid precursors.
authors.affiliation: Norwegian Defence Research Establishment, Division for Environmental Toxicology, Kjeller.
authors.forename: B
authors.initials: B
authors.lastname: Hassel
authors.forename: R E
authors.initials: RE
authors.lastname: Paulsen
authors.forename: A
authors.initials: A
authors.lastname: Johnsen
authors.forename: F
authors.initials: F
authors.lastname: Fonnum
mesh_terms: D000085:Acetates / Q000378:metabolism; D000818:Animals; D001224:Aspartic Acid / Q000378:metabolism; D002250:Carbon Radioisotopes; D002951:Citrates / Q000008:administration & dosage / Q000378:metabolism / Q000494:pharmacology*; D003342:Corpus Striatum / Q000187:drug effects / Q000378:metabolism*; D005971:Glutamates / Q000378:metabolism; D005973:Glutamine / Q000378:metabolism; D007656:Ketoglutaric Acids / Q000378:metabolism; D008297:Male; D009457:Neuroglia / Q000187:drug effects / Q000378:metabolism*; D011865:Radioisotope Dilution Technique; D051381:Rats; D011919:Rats, Inbred Strains; D012016:Reference Values; D013238:Stereotaxic Techniques; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
chemical_list: D000085:Acetates; D002250:Carbon Radioisotopes; D002951:Citrates; D005971:Glutamates; D007656:Ketoglutaric Acids; D005973:Glutamine; D001224:Aspartic Acid; C007744:fluorocitrate; D005680:gamma-Aminobutyric Acid