title: Characterization of low Mr zona pellucida binding proteins from boar spermatozoa and seminal plasma.
abstract: A group of low Mr (16 kDa-23 kDa) glycoproteins on ejaculated boar spermatozoa have been shown to have high affinity for homologous zona pellucida glycoproteins (ZPGPs). These ZPGP binding proteins are derived from seminal plasma as shown by their absence from epididymal spermatozoa and their presence in seminal plasma as identified by N-terminal amino acid sequence analysis. They bind to ZPGPs by a polysulphate recognition mechanism similar to that found for proacrosin-ZPGP interactions. The haemagglutination activity of boar seminal plasma is also associated with these low Mr glycoproteins. It is suggested that they play a role in regulating the rate of sperm capacitation and survival in the female reproductive tract.
authors.affiliation: AFRC Institute of Animal Physiology & Genetics Research, Babraham, Cambridge, England.
authors.forename: R V
authors.initials: RV
authors.lastname: Parry
authors.forename: P J
authors.initials: PJ
authors.lastname: Barker
authors.forename: R
authors.initials: R
authors.lastname: Jones
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D004527:Egg Proteins; D006023:Glycoproteins / Q000378:metabolism*; D006384:Hemagglutination; D008297:Male; D008562:Membrane Glycoproteins; D008969:Molecular Sequence Data; D011485:Protein Binding; D011956:Receptors, Cell Surface; D012661:Semen / Q000378:metabolism*; D013094:Spermatozoa / Q000378:metabolism*; D013552:Swine; D015044:Zona Pellucida / Q000378:metabolism*; D000072216:Zona Pellucida Glycoproteins
chemical_list: D004527:Egg Proteins; D006023:Glycoproteins; D008562:Membrane Glycoproteins; D011956:Receptors, Cell Surface; D000072216:Zona Pellucida Glycoproteins