[Pharmacological effects of Gosha-jinki-gan-ryo extract: effects on experimental diabetes].
Pharmacological effects of Gosha-jinki-gan-ryo extract (KJE) on experimental diabetes induced by cyproheptadine (CPH), aldose reductase activity, and experimental peripheral neuropathy were studied. The effects of KJE were compared with those of Hachimi-jio-gan-ryo extract (HJE). KJE at 417 mg/kg/day (5 times the daily dose in humans) and HJE at 367 mg/kg/day (5 times the daily dose in humans) significantly inhibited the decrease in glucose tolerance by CPH. KJE and HJE inhibited aldose reductase activity, when DL-glyceraldehyde was used as substrate, with IC50 values of 2.68 x 10(-5) g/ml and 4.45 x 10(-5) g/ml, respectively and when D-glucose was used as substrate, with IC50 values of 1.04 x 10(-4) g/ml and 1.55 x 10(-4) g/ml, respectively. KJE at 209 mg/kg/day (2.5 times the daily dose in humans) and HJE at 367 mg/kg/day significantly reduced peripheral neuropathy induced by crushing the sciatic nerve in rats. The potency of these effects of KJE was stronger than that of HJE, when a comparison was made on the basis of the daily dose.
Shoji
M
M
Research Laboratories, Kyushin Pharmaceutical Co., Ltd., Tokyo, Japan.
Sato
H
H
Hirai
Y
Y
Oguni
Y
Y
Sugimoto
C
C
Morishita
S
S
Ito
C
C
D000449:Aldehyde Reductase / Q000032:analysis; D000818:Animals; D001786:Blood Glucose / Q000187:drug effects; D003533:Cyproheptadine / Q000008:administration & dosage; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy*; D003929:Diabetic Neuropathies / Q000188:drug therapy; D004365:Drugs, Chinese Herbal / Q000494:pharmacology* / Q000627:therapeutic use; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
D001786:Blood Glucose; D004365:Drugs, Chinese Herbal; C076165:gosha-jinki-gan-ryo extract; C054591:hachimijiogan; D003533:Cyproheptadine; D000449:Aldehyde Reductase