Ionspray mass spectrometry of marine toxins. IV. Determination of diarrhetic shellfish poisoning toxins in mussel tissue by liquid chromatography/mass spectrometry.
An improved liquid chromatographic/mass spectrometric (LC/MS) method utilizing gradient elution and ion-spray ionization is described for the sensitive determination of okadaic acid and dinophysistoxin-1, the principal toxins implicated in cases of diarrhetic shellfish poisoning. The method was used to confirm the presence of both toxins, together with a recently identified isomer of okadaic acid, dinophysistoxin-2, in various samples of cultivated blue mussels (Mytilus edulis) from Canadian and European waters. The method provided a mass detection limit of 0.4 ng for each toxin, thus allowing detection of 40 ng per g of whole mussel tissue (or approximately 10 ng/g if only the digestive glands were used in the assay). Quantitative results obtained by LC/MS were in good agreement with those obtained by derivatization and high-performance liquid chromatography with fluorescence detection.
Pleasance
S
S
Institute for Marine Biosciences, National Research Council of Canada, Halifax, Nova Scotia.
Quilliam
M A
MA
Marr
J C
JC
D000818:Animals; D049872:Bivalvia; D004988:Ethers, Cyclic / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry; D008387:Marine Toxins / Q000032:analysis*; D019319:Okadaic Acid / Q000031:analogs & derivatives; D011714:Pyrans / Q000032:analysis*
D004988:Ethers, Cyclic; D008387:Marine Toxins; D011714:Pyrans; D019319:Okadaic Acid; C051904:dinophysistoxin 1; C076221:dinophysistoxin 2