title: Utilization of methoxylated benzoates and formation of intermediates by Desulfotomaculum thermobenzoicum in the presence or absence of sulfate.
abstract: Desulfotomaculum thermobenzoicum strain TSB (DSM 6193) was found to utilize some methoxylated benzoates as carbon and energy source with or without sulfate. 3- or 4-Methoxybenzoate, vanillate (4-hydroxy-3-methoxybenzoate), syringate (3,5-dimethoxy-4-hydroxybenzoate) and 3,4,5-trimethoxybenzoate were converted to corresponding hydroxybenzoates. However, neither 2-methoxybenzoate nor 2,6-dimethoxybenzoate was utilized. The organism grew acetogenically on each of the methoxylated benzoates in the absence of sulfate. 3,4-Dihydroxy-5-methoxybenzoate was detected during conversion of syringate, and syringate and 3,4-dihydroxy-5-methoxybenzoate were detected during conversion of 3,4,5-trimethoxybenzoate as intermediates. These findings indicate that 4-methoxyl-group is most readily cleaved, whereas 2-methoxyl-group is not utilized by the organism.
authors.affiliation: Institute of Technology, Shimizu Corporation, Tokyo, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Tasaki
authors.forename: Y
authors.initials: Y
authors.lastname: Kamagata
authors.forename: K
authors.initials: K
authors.lastname: Nakamura
authors.forename: E
authors.initials: E
authors.lastname: Mikami
mesh_terms: D000085:Acetates / Q000378:metabolism; D019342:Acetic Acid; D000445:Aldehyde Oxidoreductases / Q000032:analysis; D001406:Bacillaceae / Q000378:metabolism*; D001565:Benzoates / Q000378:metabolism*; D005707:Gallic Acid / Q000031:analogs & derivatives / Q000378:metabolism; D062425:Hydroxybenzoate Ethers; D062385:Hydroxybenzoates / Q000378:metabolism; D009097:Multienzyme Complexes; D014641:Vanillic Acid / Q000031:analogs & derivatives / Q000378:metabolism
chemical_list: D000085:Acetates; D001565:Benzoates; D062425:Hydroxybenzoate Ethers; D062385:Hydroxybenzoates; D009097:Multienzyme Complexes; C004520:4-anisic acid; D005707:Gallic Acid; C056267:3-methoxybenzoic acid; C001945:syringic acid; D000445:Aldehyde Oxidoreductases; C019823:carbon monoxide dehydrogenase; D014641:Vanillic Acid; D019342:Acetic Acid; C005854:3,4,5-trimethoxybenzoic acid