Transcription of the chicken anemia virus (CAV) genome and synthesis of its 52-kDa protein.
This paper describes the expression of the chicken anemia virus (CAV) genome, a recently characterized single-stranded circular-DNA virus of a new type [Noteborn et al., J. Virol. 65 (1991) 3131-3139]. The major transcript from the CAV genome is an unspliced mRNA of about 2100 nucleotides (nt). Its transcription start point and poly(A)-addition site are located at nt 354 and 2317 of the CAV sequence, respectively. In vitro translation experiments provide evidence that the major CAV open reading frame encodes a 52-kDa protein by using the fifth AUG as a start codon of the unspliced CAV mRNA.
Noteborn
M H
MH
Laboratory for Molecular Carcinogenesis, Sylvius Laboratory, University of Leiden, The Netherlands.
Kranenburg
O
O
Zantema
A
A
Koch
G
G
de Boer
G F
GF
van der Eb
A J
AJ
D015152:Blotting, Northern; D004267:DNA Viruses / Q000235:genetics*; D004279:DNA, Viral / Q000235:genetics; D005807:Genes, Overlapping / Q000235:genetics; D016679:Genome, Viral; D012333:RNA, Messenger / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics; D015183:Restriction Mapping; D014158:Transcription, Genetic / Q000235:genetics*; D014764:Viral Proteins / Q000096:biosynthesis* / Q000235:genetics
D004279:DNA, Viral; D012333:RNA, Messenger; D012367:RNA, Viral; D014764:Viral Proteins