Influence of indomethacin on histamine- and acetylcholine-induced responses in the intact and denuded epithelium of guinea pig tracheal smooth muscle.
We have investigated the effect of indomethacin on histamine- and acetylcholine (ACh)-induced responses in the intact and denuded epithelium of guinea pig isolated tracheal smooth muscle. Epithelium removal resulted in increased responsiveness to ACh and histamine. Indomethacin (2.8 microM) enhanced the sensitivity of both intact and denuded preparations to histamine and ACh. These findings suggest that the tracheal epithelium of guinea pig plays a protective role against bronchoconstrictors, such as ACh and histamine. Furthermore, indomethacin-mediated hyperresponsiveness caused by these agonists in epithelium denuded preparations might be a reflection of removal of prostaglandin (PG) biosynthesis. A similar process of interaction in indomethacin-treated asthmatic patients (with damaged airway epithelium) might take place. The significance of these findings is discussed.
Akbar
A
A
Department of Pharmacology, School of Medical Sciences, Universiti Sains Malaysia, Kelantan.
Sharma
J N
JN
D000109:Acetylcholine / Q000494:pharmacology*; D000818:Animals; D016084:Bronchoconstriction / Q000187:drug effects* / Q000502:physiology; D004848:Epithelium / Q000187:drug effects; D005260:Female; D006168:Guinea Pigs; D006632:Histamine / Q000494:pharmacology*; D007213:Indomethacin / Q000008:administration & dosage / Q000494:pharmacology*; D008297:Male; D009130:Muscle, Smooth / Q000187:drug effects*; D013223:Statistics as Topic; D014132:Trachea / Q000187:drug effects
D006632:Histamine; D000109:Acetylcholine; D007213:Indomethacin