title: [Malondialdehyde production and erythrocyte membrane resistance to free radicals, in function of adequate or inadequate protein intake, associated with different oils (sunflower, soybean, coconut, salmon)].
abstract: Requirements in polyunsaturated fatty acids (PUFA) of series n-3 and n-6 may be amplified and their metabolism, transport, and utilization may be impaired in the long term, by protein depletion. The aim of this study was to evaluate, in young rats, malondialdehyde (MDA) production and erythrocyte membrane antioxidative defense, when they were fed balanced (20% casein) or depleted (2% casein) protein diet associated with various oils (sunflower, soybean, coconut or salmon). Over a short period (28 days), eight groups of 10 male Wistar rats were fed eight different diets: TOC (20% casein + 5% sunflower oil), TOd (2% casein + 5% soybean oil), SOC (20% casein + 5% soybean oil), SOd (2% casein + 5% soybean oil), COC (20% casein + 5% coconut oil), COd (2% casein + 5% coconut oil), SAC (20% casein + 5% salmon oil), SAd (2% casein + 5% salmon oil). Blood was removed, MDA was assessed in plasma (reaction with thiobarbituric acid). Washed erythrocytes were subjected to organic free radical generator (Kit KGRL 400 Spiral R.D., Couternon, 21560 France). The haemoglobin released was analysed by spectrophotometry. The total anti-radical defense status was expressed as the length of time to reach 50% hemolysis (T 50% in min). Plasma of deficient groups (2% casein) exhibited low concentrations of protein, particularly with coconut and salmon oils; phospholipid and total cholesterol, excepted with diet containing coconut oil; triacylglycerol; and VLDL. Malondialdehyde. In groups fed balanced protein diets, the lowest values were obtained with salmon and coconut oils. MDA contents of groups TOd, COd and SAd were higher than those of their respective control groups, but significantly only in group COd. Antiradical defense status. Total anti-radical defence status in erythrocytes was not modified in the short term by balanced or depleted protein diets which ever oil was used, despite deep changes in fatty acid composition of membrane phospholipids. In particular, phospholipid contents in eicosapentaenoic, docosahexaenoic acids were greatly enhanced by the consumption of salmon oil compared to the values obtained with coconut oil.
authors.affiliation: Université de Bourgogne, Faculté des Sciences Mirande, Dijon.
authors.forename: J C
authors.initials: JC
authors.lastname: M'Fouara
authors.forename: M N
authors.initials: MN
authors.lastname: Bouziane
authors.forename: J
authors.initials: J
authors.lastname: Prost
authors.forename: J
authors.initials: J
authors.lastname: Belleville
mesh_terms: D000818:Animals; D000074263:Coconut Oil; D003059:Cocos; D004042:Dietary Fats, Unsaturated / Q000008:administration & dosage*; D004044:Dietary Proteins / Q000008:administration & dosage*; D004351:Drug Resistance; D004910:Erythrocyte Membrane / Q000187:drug effects*; D005395:Fish Oils / Q000008:administration & dosage; D005609:Free Radicals / Q000494:pharmacology*; D006368:Helianthus; D008297:Male; D008315:Malondialdehyde / Q000097:blood*; D010938:Plant Oils / Q000008:administration & dosage; D051381:Rats; D017208:Rats, Wistar; D012474:Salmon; D013024:Soybean Oil / Q000008:administration & dosage; D000074242:Sunflower Oil
chemical_list: D004042:Dietary Fats, Unsaturated; D004044:Dietary Proteins; D005395:Fish Oils; D005609:Free Radicals; D010938:Plant Oils; D000074242:Sunflower Oil; D008315:Malondialdehyde; D013024:Soybean Oil; D000074263:Coconut Oil
vernacular_title: Production de malondialdéhyde et résistance des membranes érythrocytaires aux radicaux libres, en fonction d'un apport alimentaire suffisant ou non en protéines, associé à différentes huiles (tournesol, soja, coprah, Saumon).