title: Proteolytic activity of isolated lamb calpains on myofibrils under the conditions of pH, Ca2+ concentration and temperature existing in postmortem muscle.
abstract: The two calcium-activated neutral proteinases (calpains I and II) and their specific inhibitor were isolated by ion exchange chromatography in DEAE-Sephacel from lamb skeletal muscle (longissimus dorsi). Their proteolytic activities were then determined using myofibrils as substrate. The Ca2+ requirements were different for each form of the enzyme: calpain I needed only 50 mumol Ca2+ for half-maximal activity, while the other isoenzyme, calpain II, needed 1,000 mumol Ca2+ for reaching 50% of its maximum activity. Both calpains showed a relevant activity in the pH range 5.5-6.5 (over 40% of maximum activity found at pH 7.5). With regard to the effect of temperature, both isoenzymes retained about 25% of their activity at 25 degrees C with a temperature reduction down to 4 degrees C. It is concluded that calpain I is an active protease under conditions similar to that prevalent in lamb meat during postmortem storage.
authors.affiliation: Departamento Producción Animal y Ciencia de los Alimentos, Facultad de Veterinaria, Universidad de Zaragoza, Spain.
authors.forename: P
authors.initials: P
authors.lastname: Ceña
authors.forename: I
authors.initials: I
authors.lastname: Jaime
authors.forename: J A
authors.initials: JA
authors.lastname: Beltrán
authors.forename: P
authors.initials: P
authors.lastname: Roncalés
mesh_terms: D000818:Animals; D002118:Calcium / Q000032:analysis / Q000494:pharmacology; D002154:Calpain / Q000302:isolation & purification / Q000378:metabolism*; D005519:Food Preservation; D006863:Hydrogen-Ion Concentration; D008460:Meat / Q000592:standards*; D009210:Myofibrils / Q000378:metabolism*; D011180:Postmortem Changes; D012756:Sheep; D013696:Temperature
chemical_list: D002154:Calpain; D002118:Calcium