Molecular characterization of a human anti-Rh(D) antibody with a DH segment encoded by a germ-line sequence.
The lambda-light-chain and lambda-heavy-chain variable-region genes of an anti-Rh(D) (Rh, Rhesus; D, heavy-chain diversity region) human monoclonal antibody secreted by lymphocytes transformed by the Epstein-Barr virus have been cloned and sequenced. Sequence comparison of the anti-Rh(D)mAb lambda-chain variable region with those of the other available human lambda chains revealed that it belonged to the human V lambda I (V lambda, variable region of lambda chain) subgroup. The greatest sequence similarity (80%) was observed with that of another anti-Rh antibody lambda-chain directed against the Rh(c) antigen. For the VH (VH, variable region of heavy chain) sequence, the highest similarity (86%) was observed with the germline VHG3 gene which belongs to the VHI subgroup. The expressed DH sequence of the anti-Rh(D) antibody is also of germline origin and complementarity-determining region 3 is thus produced by VH-DH and DH-JH (J, joining region) joining without recombination of multiple DH gene segments.
Chouchane
L
L
Laboratoire d'Immuno-Pharmacologie Moléculaire, CNRS, Université Paris VII, France.
Van Spronsen
A
A
Breyer
J
J
Guglielmi
P
P
Strosberg
A D
AD
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000235:genetics*; D001483:Base Sequence; D015139:Blotting, Southern; D002461:Cell Line, Transformed; D004247:DNA; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D007147:Immunoglobulin Light Chains / Q000235:genetics; D007135:Immunoglobulin Variable Region / Q000235:genetics; D008214:Lymphocytes / Q000276:immunology; D008969:Molecular Sequence Data; D012204:Rh-Hr Blood-Group System / Q000276:immunology*; D012689:Sequence Homology, Nucleic Acid
D000911:Antibodies, Monoclonal; D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains; D007135:Immunoglobulin Variable Region; D012204:Rh-Hr Blood-Group System; D004247:DNA