Production and characterization of a monoclonal antibody to human saposin C.
A murine IgG1 monoclonal antibody, termed 68-12, was produced against purified human saposin C. Immunoprecipitation and binding analysis indicated that the antibody reacted only with saposin C. Dot blotting and Western analysis demonstrated that antibody 68-12 also reacted with prosaposin and a higher molecular weight protein(s) in murine spleen and cerebral grey matter. Solid phase competitive radioimmunoassay against 125I labeled saposin C (0.25 micrograms/ml) showed no cross reactivity for saposin A, B and D up to 15 micrograms/ml. At a concentration of 50 micrograms/ml saposin A, B and D cross reacted 21, 1.5, and 49% respectively. Monoclonal antibody 68-12 appears to have potential utility in the purification, detection and quantitation of human saposin C and its precursor.
Stastny
J J
JJ
Cancer Center, University of Illinois, Chicago 60612.
Kishimoto
Y
Y
O'Brien
J S
JS
Beattie
C W
CW
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D000918:Antibody Specificity; D001667:Binding, Competitive; D015153:Blotting, Western; D003429:Cross Reactions; D006023:Glycoproteins / Q000032:analysis / Q000276:immunology*; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007074:Immunoglobulin G / Q000096:biosynthesis; D051379:Mice; D011233:Precipitin Tests; D011498:Protein Precursors / Q000276:immunology; D049231:Saposins
D000911:Antibodies, Monoclonal; D006023:Glycoproteins; D007074:Immunoglobulin G; C483345:PSAP protein, human; D011498:Protein Precursors; C483344:Psap protein, mouse; D049231:Saposins