title: Elevation of platelet-associated IgG in aplastic anemia.
abstract: We determined platelet-associated IgG (PAIgG) levels in patients with aplastic anemia, idiopathic thrombocytopenic purpura (ITP), iron deficiency anemia, and systemic lupus erythematosus (SLE), as well as in normal healthy adults as a control group. To determine PAIgG levels, we used a competitive micro enzyme-linked immunosorbent assay, which had excellent reproducibility, recovery, and dilution. We confirmed its reliability by comparing it to the immunoradiometric assay. Both the aplastic anemia group (n = 27, mean +/- SD = 218.6 +/- 244.6 ng/10(7) platelets) and the ITP group (n = 82, mean +/- SD = 212.5 +/- 327.8 ng/10(7) platelets) had higher PAIgG levels than the SLE group (n = 4, mean +/- SD = 38.4 +/- 22.4 ng/10(7) platelets), iron deficiency anemia group (n = 10, mean +/- SD = 16.1 +/- 3.6 ng/10(7) platelets), and normal control group (n = 69, mean +/- SD = 16.1 +/- 3.6 ng/10(7) platelets. The higher platelet-associated IgG levels in aplastic anemia suggest that autoimmune mechanisms are involved.
authors.affiliation: Genetic Research Laboratory, SRL, Inc., Tokyo, Japan.
authors.forename: R
authors.initials: R
authors.lastname: Kawaguchi
authors.forename: S
authors.initials: S
authors.lastname: Haruna
authors.forename: K
authors.initials: K
authors.lastname: Hikiji
authors.forename: Y
authors.initials: Y
authors.lastname: Higashi
authors.forename: Y
authors.initials: Y
authors.lastname: Tsukada
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000741:Anemia, Aplastic / Q000276:immunology*; D000747:Anemia, Hypochromic / Q000276:immunology; D015551:Autoimmunity; D001792:Blood Platelets / Q000276:immunology*; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000592:standards; D005069:Evaluation Studies as Topic; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood*; D015592:Immunoradiometric Assay; D007223:Infant; D008180:Lupus Erythematosus, Systemic / Q000276:immunology; D008297:Male; D008875:Middle Aged; D016553:Purpura, Thrombocytopenic, Idiopathic / Q000276:immunology; D012015:Reference Standards
chemical_list: D007074:Immunoglobulin G