title: Measurement of pH to quantify urease activity.
abstract: The rate of change of pH caused by the hydrolysis of urea was measured for urease solutions of 18 different concentrations. Concentrations were converted into an activity, A (measured in IU/cm3), by using a titrimetric method to assay the urease sample. For activities in the range 0.6-38 IU/cm3, A was related to the initial rate of change of pH, (dpH/dt)0, (measured in s-1) by the empirical relationship: A = 549(dpH/dt)0-1423(dpH/dt)2(0). Values of (dpH/dt)0 were sensitive to changes in the urease activity of about 0.6 IU/cm3.
authors.affiliation: Department of Medical Biophysics, University of Manchester, UK.
authors.forename: J M
authors.initials: JM
authors.lastname: Bibby
authors.forename: D W
authors.initials: DW
authors.lastname: Hukins
mesh_terms: D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007700:Kinetics; D010940:Plant Proteins / Q000378:metabolism*; D014508:Urea / Q000378:metabolism; D014510:Urease / Q000378:metabolism*
chemical_list: D010940:Plant Proteins; D014508:Urea; D014510:Urease