Transforming growth factor e: amino acid analysis and partial amino acid sequence.
Our previous studies have demonstrated that transforming growth factor e (TGFe) acts as a mitogen for epithelial and fibroblastic cells in both monolayer and soft agar. We have also identified TGFe in both normal and neoplastic tissues of mostly epithelial origin, and in body fluids. In this study we report on the purification of TGFe to homogeneity from bovine kidney using a multistep purification protocol which utilizes high performance electrophoresis chromatography in the final step. Amino acid analysis of TGFe revealed high content of proline, aspartate and glutamate. Examination of partial amino acid sequence indicated no similarity to other, already characterized, growth factors.
Parnell
P G
PG
Department of Pathology, College of Veterinary Medicine, University of Georgia, Athens 30602.
Wunderlich
J
J
Carter
B
B
Halper
J
J
D000306:Adrenal Cortex Neoplasms; D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002417:Cattle; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D006027:Glycosides; D006801:Humans; D007668:Kidney / Q000737:chemistry / Q000502:physiology; D008969:Molecular Sequence Data; D015291:Transforming Growth Factors / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology
D000596:Amino Acids; D006027:Glycosides; C065911:transforming growth factor type e; D015291:Transforming Growth Factors