Rapid separation of DNA molecules by agarose gel electrophoresis: use of a new agarose matrix and a survey of running buffer effects.
This report describes the use of a new type of agarose (FastLane agarose) for faster separation of DNA by agarose gel electrophoresis. DNA molecules separated in this agarose exhibited electrophoretic mobilities up to 30% higher than similar separations in standard analytical grade agarose. DNA molecules of all sizes examined showed higher mobilities in FastLane agarose. The mobility increase was predominantly due to the low electroendosmosis of FastLane agarose and was most pronounced in pulsed field gel electrophoresis separations. The magnitude of mobility increase varied depending on the conditions used for electrophoresis.
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FMC Bio-Products, Rockland, ME 04841.
D001888:Boric Acids; D002021:Buffers; D004247:DNA / Q000302:isolation & purification*; D004271:DNA, Fungal / Q000302:isolation & purification; D004492:Edetic Acid; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods*; D012441:Saccharomyces cerevisiae; D012685:Sepharose; D014325:Tromethamine
D001888:Boric Acids; D002021:Buffers; D004271:DNA, Fungal; C069591:Tris-borate-EDTA buffer; D014325:Tromethamine; D004247:DNA; D012685:Sepharose; D004492:Edetic Acid