title: Determination of procaine in equine plasma and urine by high-performance liquid chromatography.
abstract: The variability in plasma and urine equine procaine measurement between three independent laboratories using current methods led to the development of a sensitive, reliable, and reproducible high-performance liquid chromatographic method. Standardbred mares were administered either a penicillin G procaine preparation intramuscularly or procaine hydrochloride subcutaneously, and blood and urine were collected at defined time intervals. By HPLC the detection limits for procaine in plasma and urine were 1 and 10 ng/mL, respectively. In contrast procaine in plasma could not be detected by GC-NPD, while the urinary detection limit was 50 ng/mL. The concentration of fluoride in the collection tubes and repetitive freeze-thawing modified plasma procaine measurement. Urinary pH was a factor in estimation of urine procaine levels with greater recovery and reproducibility of results at pH 5 as compared to pH 7. This HPLC method provides a simple, sensitive, and reliable quantitation of procaine in equine plasma and urine.
authors.affiliation: Race Track Division, Agriculture Canada, Ottawa and Jerseyville, Ontario.
authors.forename: A J
authors.initials: AJ
authors.lastname: Stevenson
authors.forename: M P
authors.initials: MP
authors.lastname: Weber
authors.forename: F
authors.initials: F
authors.lastname: Todi
authors.forename: M
authors.initials: M
authors.lastname: Mendonca
authors.forename: J D
authors.initials: JD
authors.lastname: Fenwick
authors.forename: L
authors.initials: L
authors.lastname: Young
authors.forename: E
authors.initials: E
authors.lastname: Kwong
authors.forename: F
authors.initials: F
authors.lastname: Chen
authors.forename: P
authors.initials: P
authors.lastname: Beaumier
authors.forename: S
authors.initials: S
authors.lastname: Timmings
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000662:veterinary*; D004300:Doping in Sports; D006736:Horses / Q000378:metabolism*; D011343:Procaine / Q000032:analysis* / Q000097:blood / Q000652:urine
chemical_list: D011343:Procaine