title: Isolation and characterization of a 25 kDa antifungal protein from flax seeds.
abstract: We have purified a 25 kDa protein from flax seeds to homogeneity by polyethyleneimine precipitation, ammonium sulfate precipitation, chitin extraction, Mono S cation exchange and C18 reversed phase column chromatographies. The purified protein strongly inhibited the growth of the agronomically important pathogen Alternaria solani, the causative agent of tomato early blight and in synergy with nikkomycin Z strongly inhibited the human pathogen Candida albicans. Amino terminal sequence analysis of the purified protein indicated that it has a high degree of homology to other reported pathogenesis-related antifungal proteins.
authors.affiliation: Department of Protein Biochemistry, Monsanto Company, St. Louis, MO 63198.
authors.forename: J R
authors.initials: JR
authors.lastname: Borgmeyer
authors.forename: C E
authors.initials: CE
authors.lastname: Smith
authors.forename: Q K
authors.initials: QK
authors.lastname: Huynh
mesh_terms: D000528:Alternaria / Q000187:drug effects; D000595:Amino Acid Sequence; D000617:Aminoglycosides; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000935:Antifungal Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D002176:Candida albicans / Q000187:drug effects; D011232:Chemical Precipitation; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D004357:Drug Synergism; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010940:Plant Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D012639:Seeds / Q000737:chemistry*; D012689:Sequence Homology, Nucleic Acid
chemical_list: D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D010940:Plant Proteins; C011952:nikkomycin