Suppression of audiogenic epileptic seizures by intracerebral injection of a CCK gene vector.
P77PMC rat is a breed of rat with congenital audiogenic seizure (AS). AS attacks could be suppressed by cholecystokinin octapeptide (CCK-8) injected intracerebroventricularly (i.c.v.). In the present study we made i.c.v. injection of plasmid pSV2-beta Gal or pSV2-CCK encapsulated with lipofectin. Expression of pSV2-beta Gal in brain occurred from d 1 to d 14, with maximal expression at d 3 and d 4. After i.c.v. injection of pSV2-CCK plasmid, the AS of the P77PMC rats was markedly reduced, which was most obvious at d 3 and d 4. The time course of the AS repression was almost identical with that of pSV2-beta Gal expression. The results suggest that the repression of the AS in P77PMC rats is accounted for by the expression of foreign CCK gene in brain tissue.
Zhang
L X
LX
Neuroscience Research Centre, Beijing Medical University, China.
Wu
M
M
Han
J S
JS
D000161:Acoustic Stimulation; D000818:Animals; D002552:Cerebral Ventricles / Q000502:physiology / Q000503:physiopathology*; D002766:Cholecystokinin / Q000235:genetics / Q000502:physiology*; D004247:DNA / Q000008:administration & dosage / Q000235:genetics; D004827:Epilepsy / Q000503:physiopathology* / Q000517:prevention & control; D015316:Genetic Therapy; D005822:Genetic Vectors; D010957:Plasmids; D051381:Rats; D011922:Rats, Mutant Strains; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012640:Seizures / Q000503:physiopathology* / Q000517:prevention & control; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
D011993:Recombinant Fusion Proteins; D004247:DNA; D002766:Cholecystokinin; D001616:beta-Galactosidase