An assessment of cadmium toxicity on cytochrome P-450 and flavin monooxygenase-mediated metabolic pathways of dimethylaniline in male rabbits.
Cadmium is an environmental pollutant and its effect on the in vitro metabolism of N,N-dimethylaniline (DMA) using male rabbits was investigated. Activities of cytochrome P-450 and FMO-dependent monooxygenases were studied using hepatic microsomes. Following CdCl2 (i.p.) administration (6 mg/kg/day for 6 days), both DMA-N-oxidation and DMA-N-demethylation decreased by 86%. The effects of CdCl2 on the phenobarbitone (PB)-induced form of P-450 were also studied. Intraperitoneal pretreatment of rabbits with PB (5 mg/kg/day for 5 days) increased N-demethylation by 82%, while N-oxidation decreased by 49%. Both reactions decreased significantly on additional treatment with CdCl2. Promethazine (5 mg/kg/day for 5 days) did not produce any change in the activities of either enzyme. The enzymes remained unaffected by CdCl2 treatment in promethazine-pretreated animals thus confirming its role as a hepatoprotective agent.
Anjum
F
F
Zoology Department, University of the Punjab, Lahore, Pakistan.
Raman
A
A
Shakoori
A R
AR
Gorrod
J W
JW
D000814:Aniline Compounds / Q000378:metabolism*; D000818:Animals; D002104:Cadmium / Q000633:toxicity*; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D004781:Environmental Exposure; D004789:Enzyme Activation / Q000187:drug effects; D008297:Male; D008745:Methylation; D010084:Oxidation-Reduction; D010105:Oxygenases / Q000378:metabolism*; D010634:Phenobarbital; D011398:Promethazine; D011817:Rabbits
D000814:Aniline Compounds; D002104:Cadmium; C015157:N,N-dimethylaniline; D003577:Cytochrome P-450 Enzyme System; D010105:Oxygenases; C020066:dimethylaniline monooxygenase (N-oxide forming); D011398:Promethazine; D010634:Phenobarbital