Determination of pentoxifylline and its metabolites in human plasma by high-performance liquid chromatography with solid-phase extraction.
A simple and reliable method for the determination of pentoxifylline and its main metabolites in human plasma has been developed using high-performance liquid chromatography. After selective solid-phase extraction, pentoxifylline, its metabolites and an internal standard, 7-(2'-chloroethyl)theophylline, were separated on a 5-micron LiChrospher 100 RP-18 column using water-dioxan-acetonitrile (87:6.5:6.5, v/v/v) acidified with acetic acid (0.5%, v/v) as the mobile phase. The analytes were detected at 275 nm. The lowest detectable concentration for all analytes was 25 ng/ml; the recovery was 85%. The assay has been successfully applied to analysis of these compounds in human plasma after administration of an oral dose of 400 mg of pentoxifylline to healthy volunteers.
Mancinelli
A
A
Drug Metabolism and Pharmacokinetic Department, Pomezia, Rome, Italy.
Pace
S
S
Marzo
A
A
Martelli
E A
EA
Passetti
G
G
D000284:Administration, Oral; D000704:Analysis of Variance; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D010431:Pentoxifylline / Q000008:administration & dosage / Q000097:blood* / Q000737:chemistry
D010431:Pentoxifylline