Effects of caffeine on the DNA and protein synthesis of the protein-energy malnourished neonatal cardiac muscle cells in culture.
1. The growth of cardiac cells derived from newborn rats whose dams were either malnourished or malnourished with caffeine during pregnancy was inhibited in culture over the period of 5 days as compared to that of the normally nourished cells. 2. Cells derived from malnourished rats with caffeine added to their diets showed a greater inhibition than those from the malnourished rats not given caffeine. 3. Both DNA and protein synthesis showed an inhibition due to caffeine in a dose-dependent manner using normally nourished cells. 4. In the presence of exogenous 2 mM caffeine, the degree of percent inhibition of DNA and protein synthesis of cells derived from rats malnourished with caffeine was less than that from the rats with malnutrition alone. 5. The present data indicated that malnutrition combined with caffeine during pregnancy exerted a greater negative effect on the nature of cell growth than malnutrition alone and these cells became less sensitive to exogenous caffeine.
Kanemaru
Y
Y
Department of Physiology, Pediatrics and Anatomy, Louisiana State University Medical Center, New Orleans 70119.
Rossowska
M J
MJ
Yoshino
S
S
Yazdani
M
M
Narayanan
C H
CH
Nakamoto
T
T
D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism*; D002110:Caffeine / Q000494:pharmacology*; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis*; D005260:Female; D006321:Heart / Q000187:drug effects; D007930:Leucine / Q000378:metabolism; D009124:Muscle Proteins / Q000096:biosynthesis*; D009206:Myocardium / Q000378:metabolism*; D011247:Pregnancy; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D011502:Protein-Energy Malnutrition / Q000378:metabolism*; D051381:Rats; D013936:Thymidine / Q000378:metabolism
D009124:Muscle Proteins; D011500:Protein Synthesis Inhibitors; D002110:Caffeine; D004247:DNA; D007930:Leucine; D013936:Thymidine