Activation of lipoprotein lipase in cardiac myocytes by glycosylation requires trimming of glucose residues in the endoplasmic reticulum.
Incubation of cycloheximide-treated cardiac myocytes results in a time-dependent increase in cellular and heparin-releasable lipoprotein lipase (LPL) activities. N-Methyldeoxynojirimycin (1 mM) and castanospermine (100 micrograms/ml), inhibitors of glucosidases in the endoplasmic reticulum (ER), prevented the increase in cellular LPL activity. The glucosidase inhibitors did not influence the synthesis or turnover of LPL protein. Therefore activation of LPL by glycosylation in cardiac myocytes requires the trimming of glucose residues in oligosaccharide chains by glucosidases of the ER.
Carroll
R
R
MRC Signal Transduction Group, Faculty of Medicine, University of Calgary, Alberta, Canada.
Ben-Zeev
O
O
Doolittle
M H
MH
Severson
D L
DL
D017485:1-Deoxynojirimycin / Q000031:analogs & derivatives*; D000818:Animals; D003513:Cycloheximide / Q000494:pharmacology; D004721:Endoplasmic Reticulum / Q000201:enzymology*; D004789:Enzyme Activation; D005944:Glucosamine / Q000031:analogs & derivatives / Q000494:pharmacology; D005947:Glucose / Q000378:metabolism*; D005959:Glucosidases / Q000037:antagonists & inhibitors / Q000378:metabolism; D006031:Glycosylation; D007212:Indolizines / Q000494:pharmacology; D007700:Kinetics; D008071:Lipoprotein Lipase / Q000378:metabolism*; D008297:Male; D009206:Myocardium / Q000201:enzymology*; D051381:Rats; D011919:Rats, Inbred Strains; D054836:Indolizidines
D017485:1-Deoxynojirimycin; D003513:Cycloheximide; D005944:Glucosamine; D005947:Glucose; D005959:Glucosidases; D007212:Indolizines; D008071:Lipoprotein Lipase; C037806:castanospermine; D054836:Indolizidines; C033424:N-methyldeoxynojirimycin