Stage-specific tyrosine phosphorylation of actin in Dictyostelium discoideum cells.
A 45 kDa protein in Dictyostelium discoideum cells that was recognized by a phosphotyrosine-specific antibody was identified by its binding activity to DNase I and its 2D-electrophoretic behavior as actin. The reactivity of actin with the antibody was transiently enhanced for about 30 minutes shortly after starving cells were reintroduced into nutrient medium. This effect indicates a modification of actin that is regulated under physiological conditions. A similar effect was obtained when growing cells were treated with phenylarsine oxide (PAO), an inhibitor of phosphotyrosine phosphatases. This effect was reversed and the cells fully recovered upon addition of the PAO antagonist 2,3-dimercaptopropanol. Starved cells did not show this enhancement of antibody labelling, which indicates that the response to PAO depends on the developmental stage. Phosphorylated amino acid residues were identified after in vivo labelling with [32P]phosphate in the presence of PAO. Part of the radioactivity in the actin band was recovered as phosphotyrosine, another part as phosphoserine. PAO caused the cells to form elongated blebs, to round up and finally to become immobilized. Fluorescence labelling with phalloidin of cells that were fixed at different times of PAO treatment revealed a progressive decrease in the staining for actin filaments and showed that these alterations in cytoskeleton organization were readily reversible, in accordance with the reversal of tyrosine phosphorylation at actin.
Schweiger
A
A
Max-Planck-Institut für Biochemie, Martinsried bei München, Germany.
Mihalache
O
O
Ecke
M
M
Gerisch
G
G
D000199:Actins / Q000378:metabolism*; D000818:Animals; D000906:Antibodies; D001152:Arsenicals / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects; D003599:Cytoskeleton / Q000187:drug effects; D004023:Dictyostelium / Q000254:growth & development* / Q000378:metabolism; D010766:Phosphorylation / Q000187:drug effects; D014443:Tyrosine / Q000378:metabolism*; D015854:Up-Regulation
D000199:Actins; D000906:Antibodies; D001152:Arsenicals; C029341:oxophenylarsine; D014443:Tyrosine