Biosynthesis of pyrrolnitrin. Incorporation of 13C, 15N double-labelled D- and L-tryptophan.
Experiments on the incorporation of D- and L-[alanine-3-13C,2-15N]tryptophan into the antibiotic pyrrolnitrin in Pseudomonas aureofaciens confirmed earlier conclusions about the conversion of L-tryptophan into pyrrolnitrin. They also demonstrated that a fraction of the D isomer is incorporated without breakage of the 15N-carbon bond, consistent with the operation of a second pathway from D-tryptophan to pyrrolnitrin. Cell-free experiments confirmed the conversion of 3-(o-aminophenyl)pyrrole into aminopyrrolnitrin but failed to detect enzymatic oxidation of the latter to pyrrolnitrin.
Zhou
P
P
Department of Chemistry, University of Washington, Seattle 91895.
Mocek
U
U
Siesel
B
B
Floss
H G
HG
D000409:Alanine / Q000378:metabolism; D002247:Carbon Isotopes; D004151:Dipeptides / Q000378:metabolism; D009587:Nitrogen Isotopes; D011549:Pseudomonas / Q000737:chemistry / Q000378:metabolism*; D011764:Pyrrolnitrin / Q000096:biosynthesis*; D013237:Stereoisomerism; D014364:Tryptophan / Q000737:chemistry / Q000378:metabolism*
D002247:Carbon Isotopes; D004151:Dipeptides; D009587:Nitrogen Isotopes; D014364:Tryptophan; D011764:Pyrrolnitrin; D000409:Alanine