Prevention of growth of Yersinia enterocolitica in blood by polyester fiber filtration.
The ability of polyester white cell-reduction blood filters to prevent the growth of Yersinia enterocolitica in units of donated blood was studied. Sixteen units of freshly drawn blood were inoculated with 10, 50, 100, or 150 colony-forming units (CFU) per mL of a clinical isolate of Y. enterocolitica (serotype O:3). The units were subsequently fractionated into red cell concentrate and resuspended in AS-1 or AS-3 solution. One-half of the red cell concentrates in each solution were filtered within 15 hours of phlebotomy and stored for 42 days. The remaining units served as unfiltered controls. Bacterial growth was monitored by weekly cultures and, on the last storage day, by the presence of endotoxin and the formation of methemoglobin. One hundred twelve primary cultures (560 plates) were performed. Units collected in AS-1 and filtered remained sterile when initially inoculated with 50 CFU or less. Filtered units spiked with 100 CFU or less and collected in AS-3 remained sterile throughout their shelf life. All unfiltered units supported bacterial growth and the formation of endotoxin and methemoglobin. The filtration of freshly donated blood proves to limit the growth of Y. enterocolitica in red cell components.
Wenz
B
B
Department of Laboratory Medicine, Albert Einstein College of Medicine, New York, New York.
Burns
E R
ER
Freundlich
L F
LF
D001784:Blood Gas Analysis; D002482:Cellulose / Q000031:analogs & derivatives; D004731:Endotoxins / Q000097:blood; D004912:Erythrocytes / Q000382:microbiology; D005374:Filtration / Q000295:instrumentation / Q000379:methods; D006801:Humans; D011091:Polyesters; D015009:Yersinia Infections / Q000517:prevention & control*; D015008:Yersinia enterocolitica / Q000254:growth & development*
D004731:Endotoxins; D011091:Polyesters; C005062:acetylcellulose; D002482:Cellulose