Preservation of the diffusible cations for secondary ion mass spectrometry. II. Artefacts in material embedded in araldite or melamine.
Flotation on hot water (about 60 degrees C) which is frequently employed to stretch semithin sections on substrates for SIMS (secondary ion mass spectrometry) microscopy, is the cause of numerous artefacts. In the case of epoxy resin-embedded tissue, one observes loss of potassium and sodium and accumulation of calcium. The relative contrast of cell nuclei in the ionic images, is rapidly affected by these ion migrations. After prolonged contact with hot water, tissue becomes uniformly emissive. In the case of hydrosoluble resin-embedded tissue, potassium and sodium do not appear to be affected by the action of water, which suggests that they are covalently bound with chelating sites buried beneath the layer of water bound to the surface of the macromolecules. Calcium accumulates, probably on widely exposed anionic sites. Moreover, the domains observed in hydrosoluble resin-embedded tissue shrink differently according to the proportion of water removed by melamine; this can provide interesting information on the initial equilibrium between water, ion sand macromolecules. Our results seem to support the assumption that bound water should play an important role in the preservation of both macromolecular architecture and ion distributions.
Mentré
P
P
Centre de Biologie Cellulaire, CNRS, Ivry-sur-Seine, France.
Escaig
F
F
D000818:Animals; D000965:Antimony; D016477:Artifacts; D002412:Cations / Q000032:analysis; D004058:Diffusion; D004853:Epoxy Resins; D007668:Kidney / Q000737:chemistry / Q000648:ultrastructure; D013058:Mass Spectrometry / Q000379:methods*; D051379:Mice; D008853:Microscopy / Q000379:methods*; D010796:Phthalic Anhydrides; D012117:Resins, Synthetic; D016610:Tissue Embedding / Q000379:methods*; D016707:Tissue Fixation; D014227:Triazines; D014867:Water
D002412:Cations; D004853:Epoxy Resins; D010796:Phthalic Anhydrides; D012117:Resins, Synthetic; D014227:Triazines; D014867:Water; C009133:pyroantimonate; C005752:araldite; D000965:Antimony; C011907:melamine