title: Effect of dialyser composition and reuse on neutrophil count and elastase alpha-1 proteinase inhibitor complex formation.
abstract: To assess the inter-relationship of leucopenia and PMN elastase release we undertook a prospective crossover study of 6 patients dialysed with new and reused cuprophane, cellulose acetate and polysulfone membranes. Serial blood samples were analysed for PMN count, and elastase-alpha 1-proteinase inhibitor complex (E alpha 1PI) concentrations. After 15 min dialysis with new membranes median PMN counts fell by 72.2%, 25.3% and 22.1% with cuprophane, cellulose and polysulfone, respectively. With reuse the decreases were reduced to 6.4%, 8% and 13.6%. All membranes produced a gradual increase of E alpha 1PI. Median E alpha 1PI accumulation rates (ng ml-1 min-1) with new membranes were 175, 169 and 187 for cuprophane, cellulose acetate and polysulfone, respectively. With reuse of cuphrophane and cellulose acetate these rates fell to 99 and 109 (p less than 0.05 and p less than 0.05, respectively), however, with polysulfone it remained unchanged at 180 ng ml-1 min-1. This study highlights differences between two aspects of the neutrophil response to haemodialysis, and demonstrates that extrapolation from individual parameters to conclusions concerning biocompatibility may be inappropriate.
authors.affiliation: Institute of Nephrology, Royal Infirmary, Cardiff, Wales, UK.
authors.forename: K L
authors.initials: KL
authors.lastname: Donovan
authors.forename: S
authors.initials: S
authors.lastname: Pacholok
authors.forename: A
authors.initials: A
authors.lastname: Phillips
authors.forename: J L
authors.initials: JL
authors.lastname: Humes
authors.forename: G A
authors.initials: GA
authors.lastname: Coles
authors.forename: J D
authors.initials: JD
authors.lastname: Williams
mesh_terms: D000328:Adult; D000368:Aged; D001672:Biocompatible Materials; D002482:Cellulose / Q000031:analogs & derivatives; D005260:Female; D005374:Filtration / Q000295:instrumentation; D006801:Humans; D007958:Leukocyte Count; D008297:Male; D008567:Membranes, Artificial; D008875:Middle Aged; D009503:Neutropenia / Q000097:blood / Q000209:etiology; D009504:Neutrophils / Q000201:enzymology*; D010196:Pancreatic Elastase / Q000037:antagonists & inhibitors* / Q000097:blood; D011108:Polymers; D006435:Renal Dialysis / Q000009:adverse effects / Q000295:instrumentation*; D015842:Serine Proteinase Inhibitors / Q000097:blood*; D013450:Sulfones
chemical_list: D001672:Biocompatible Materials; D008567:Membranes, Artificial; D011108:Polymers; D015842:Serine Proteinase Inhibitors; D013450:Sulfones; C017662:polysulfone P 1700; C005062:acetylcellulose; D002482:Cellulose; C009865:cuprammonium cellulose; D010196:Pancreatic Elastase