Induction of rat hepatic and intestinal glutathione S-transferases by dietary butylated hydroxyanisole.
To obtain insight into the protection mechanism of butylated hydroxyanisole (BHA), a widely used food preservative with anticarcinogenic properties, we investigated the effects of dietary BHA on rat hepatic and intestinal glutathione S-transferase (GST) enzyme activity, and GST isozyme levels. In the proximal small intestine and liver, BHA supplementation significantly increased GST enzyme activity as compared with controls (2.3- and 1.7-fold, respectively, P less than 0.05). GST class alpha and mu contents were significantly higher only in the small intestine (1.6-2.1-fold and 1.3-1.5-fold, respectively, P less than 0.05), whereas GST class pi was significantly induced in liver (4.6-fold, P less than 0.05).
Nijhoff
W A
WA
Division of Gastroenterology, University Hospital, St Radboud, Nijmegen, The Netherlands.
Peters
W H
WH
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002083:Butylated Hydroxyanisole / Q000008:administration & dosage / Q000494:pharmacology*; D003106:Colon / Q000187:drug effects* / Q000201:enzymology; D004032:Diet; D004790:Enzyme Induction / Q000187:drug effects; D005982:Glutathione Transferase / Q000096:biosynthesis*; D007421:Intestine, Small / Q000187:drug effects* / Q000201:enzymology; D007527:Isoenzymes / Q000096:biosynthesis; D008099:Liver / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
D000970:Antineoplastic Agents; D007527:Isoenzymes; D002083:Butylated Hydroxyanisole; D005982:Glutathione Transferase