Double-stranded dideoxy sequencing from "dirty" DNA--done in a day.
A rapid method for preparing and directly sequencing plasmid and phagemid miniprep DNA is described. This protocol is a novel combination of two fairly standard procedures, resulting in quick and easy generation of sequence data. The lack of extensive manipulations in the purification process allows the production of DNA sequence data in a single day.
Disotell
T R
TR
Department of Anthropology, Harvard University, Cambridge, MA 02138.
Zehr
S M
SM
Ruvolo
M
M
D000818:Animals; D001483:Base Sequence; D002520:Cercopithecidae; D003001:Cloning, Molecular; D004247:DNA; D005821:Genetic Techniques; D002679:Pan troglodytes; D010957:Plasmids / Q000235:genetics*; D016133:Polymerase Chain Reaction; D013997:Time Factors
D004247:DNA