title: Optimization of a model of full-thickness epidermal burns in the pig and immunohistochemical study of epidermodermal junction regeneration during burn healing.
abstract: In order to obtain a wound model in which healing involved epidermis rebuilding and epidermodermal junction (EDJ) regeneration without involvement of any dermal repair, we optimized a previous model of experimental cutaneous burning with an aluminum bar by testing various conditions of burning associated with different pre- and postburn skin treatments. On the optimized model of full-thickness epidermal burns without any dermal injury, we investigated the kinetics of regeneration of 4 EDJ components, from day 2 to day 23 after burning. The epidermal healing was studied by light microscopy and EDJ regeneration by indirect immunofluorescence with one bullous pemphigoid (BP) serum, antisera to fibronectin and to type IV collagen (Coll IV) and the monoclonal antibody 4C 12-8 to laminin. Histologically, neoepidermis, detected from day 2, appeared as a reepidermization tongue which progressed from the burn edges between the overlying necrotic burned epidermis and the underlying uninjured dermis. Epidermis continuity was found to be restored at day 9. Immunohistochemically, labelling of BP antigen (BPA), Coll IV and laminin extended all along the neo-EDJ, from day 2 to day 23. In contrast, fibronectin labelling was detected only in the proximal and median portions of the neo-EDJ before day 7, then all along the neo-EDJ, from day 7 to day 23. For all the components except Coll IV, the intensity of the labelling beneath the neoepidermis was higher than that of the residual labelling remaining under the necrotic epidermis. Therefore, BPA and laminin regenerated synchronously to neoepidermis whereas fibronectin first regenerated with delay, then synchronously.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Laboratoire d'Anatomie et de Cytologie Pathologiques, CHU Rangueil; Toulouse, France.
authors.forename: C
authors.initials: C
authors.lastname: Rigal
authors.forename: M T
authors.initials: MT
authors.lastname: Pieraggi
authors.forename: G
authors.initials: G
authors.lastname: Serre
authors.forename: H
authors.initials: H
authors.lastname: Bouissou
mesh_terms: D000818:Animals; D001324:Autoantigens / Q000032:analysis; D002056:Burns / Q000503:physiopathology*; D002352:Carrier Proteins; D003094:Collagen / Q000032:analysis; D003598:Cytoskeletal Proteins; D000071821:Dystonin; D004817:Epidermis / Q000737:chemistry / Q000473:pathology / Q000502:physiology*; D005260:Female; D005353:Fibronectins / Q000032:analysis; D007150:Immunohistochemistry; D007797:Laminin / Q000032:analysis; D008854:Microscopy, Electron; D008954:Models, Biological; D009419:Nerve Tissue Proteins; D024041:Non-Fibrillar Collagens; D012038:Regeneration / Q000502:physiology*; D012867:Skin / Q000737:chemistry / Q000473:pathology; D012879:Skin Physiological Phenomena; D013552:Swine; D014945:Wound Healing / Q000502:physiology; D000097805:Collagen Type XVII
chemical_list: D001324:Autoantigens; D002352:Carrier Proteins; D003094:Collagen; D003598:Cytoskeletal Proteins; D000071821:Dystonin; D005353:Fibronectins; D007797:Laminin; D009419:Nerve Tissue Proteins; D024041:Non-Fibrillar Collagens; D000097805:Collagen Type XVII; C095667:Dst protein, mouse