title: On the mechanism of the mitochondrial decarboxylation of phosphatidylserine.
abstract: To study intramitochondrial phospholipid flow, radiolabeled phosphatidylserine was introduced into isolated rat liver mitochondria from donor vesicles through the action of a nonspecific lipid transfer protein. Imported phosphatidylserine was rapidly decarboxylated to phosphatidylethanolamine. Both the imported phosphatidylserine and the formed phosphatidylethanolamine were confined to the outer membrane. The enzyme phosphatidylserine decarboxylase was shown to be located exclusively in the inner membrane. It was not enriched in isolated contact site fractions. 1,4-Dinitrophenol caused an inhibition of the decarboxylation of phosphatidylserine. This inhibition was not due to the uncoupling of the oxidative phosphorylation itself, but possibly due to a decrease in the number of contact sites. This suggests that phosphatidylserine flows from the outer membrane to the inner membrane through contact sites between inner and outer membrane to become decarboxylated and that the formed phosphatidylethanolamine flows directly back to the outer membrane, without mixing with inner membrane phosphatidylethanolamine.
authors.affiliation: Department of Membrane Biochemistry, University of Utrecht, The Netherlands.
authors.forename: R
authors.initials: R
authors.lastname: Hovius
authors.forename: B
authors.initials: B
authors.lastname: Faber
authors.forename: B
authors.initials: B
authors.lastname: Brigot
authors.forename: K
authors.initials: K
authors.lastname: Nicolay
authors.forename: B
authors.initials: B
authors.lastname: de Kruijff
mesh_terms: D000818:Animals; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone / Q000494:pharmacology; D002262:Carboxy-Lyases / Q000378:metabolism*; D007425:Intracellular Membranes / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008563:Membrane Lipids / Q000378:metabolism*; D008930:Mitochondria, Liver / Q000378:metabolism*; D009596:Nitrophenols / Q000494:pharmacology; D010085:Oxidative Phosphorylation / Q000187:drug effects; D010718:Phosphatidylserines / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013367:Submitochondrial Particles / Q000187:drug effects / Q000378:metabolism*
chemical_list: D008563:Membrane Lipids; D009596:Nitrophenols; D010718:Phosphatidylserines; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone; D002262:Carboxy-Lyases; C022353:phosphatidylserine decarboxylase