title: HLA-DQA1 allele and genotype frequencies in a northern Italian population.
abstract: HLA-DQA1 typing of 227 randomly selected Northern Italian people by the use of polymerase chain reaction are reported. The combined use of commercial Amplitype HLA-DQalpha system and four sequence-specific oligonucleotide probes allows the definition of 8 alleles and 36 genotypes, arranged according to World Health Organisation nomenclature. Seven of these genotypes are not observed among the analyzed samples. Allele frequencies range from 1.5 to 35.7% and genotype observations do not deviate significantly from Hardy-Weinberg equilibrium; observed heterozygosity is 0.8238 with an allelic diversity value of 0.79 and the power of discrimination is 0.925. Our Italian population sample shows differences from other Caucasian samples both for allele and genotype frequencies. This locus typing for the 8 defined alleles provides a rapid and sensitive method in individual identification and paternity investigation.
authors.affiliation: Istituto di Medicina Legale, Universitá di Genova, Italy.
authors.forename: F
authors.initials: F
authors.lastname: De Stefano
authors.forename: L
authors.initials: L
authors.lastname: Casarino
authors.forename: A
authors.initials: A
authors.lastname: Mannucci
authors.forename: L
authors.initials: L
authors.lastname: Delfino
authors.forename: M
authors.initials: M
authors.lastname: Canale
authors.forename: G B
authors.initials: GB
authors.lastname: Ferrara
mesh_terms: D000483:Alleles; D001483:Base Sequence; D005784:Gene Amplification; D005838:Genotype; D006683:HLA-DQ Antigens / Q000032:analysis*; D059848:HLA-DQ alpha-Chains; D006801:Humans; D007558:Italy; D008969:Molecular Sequence Data; D010334:Paternity; D016133:Polymerase Chain Reaction; D009626:Terminology as Topic; D044465:White People / Q000235:genetics*; D014944:World Health Organization
chemical_list: D006683:HLA-DQ Antigens; D059848:HLA-DQ alpha-Chains; C069050:HLA-DQA1 antigen