A note on the use of a plasmid as a DNA probe in the detection of a Lactobacillus fermentum strain in porcine stomach contents.
A plasmid (about 50 kb) was used as a DNA probe to enumerate, by colony hybridization, a strain of Lactobacillus fermentum in the stomach contents of eight piglets. The population sizes obtained by colony hybridization were in agreement with estimated levels calculated on the basis of plasmid profiling of colonies isolated at random from the total lactobacillus population.
Tannock
G W
GW
Department of Microbiology, University of Otago, Dunedin, New Zealand.
McConnell
M A
MA
Fuller
R
R
D000818:Animals; D015342:DNA Probes; D005766:Gastrointestinal Contents / Q000382:microbiology*; D014644:Genetic Variation; D007778:Lactobacillus / Q000302:isolation & purification*; D009693:Nucleic Acid Hybridization; D010957:Plasmids / Q000235:genetics*; D013552:Swine / Q000382:microbiology*
D015342:DNA Probes