A comparison of a solid phase IRC assay and the PSIFT for detection of antibodies to platelets.
A rapid solid phase indicator red cells assay (IRCA) for detection of platelet antibodies was developed and its sensitivity compared with PSIFT. Platelets were attached to the surface of polystyrene microtitre plate wells by means of a sodium carbonate buffer and centrifugation. Uncovered areas were blocked by a gelatin blocking buffer. After serum incubation bound platelet-specific antibodies were made visible by anti-IgG-coated indicator red cells and a brief centrifugation. A positive result, meaning the presence of an anti-platelet antibody was indicated by red cell adherence over the reaction surface. In the absence of serum antibodies to platelets the indicator red cells formed a pellet. The IRCA showed a high sensitivity; the anti-platelet antibody Thrombocyte was detectable until a dilution of 1:1,600 whereas the same antibody in the PSIFT could only be detected until a dilution of 1:400.
Häcker-Shahin
B
B
Blutspendezentrale des Städtischen Klinikums Karlsruhe, Universität Freiburg, Germany.
Giannitsis
D J
DJ
D001792:Blood Platelets / Q000276:immunology*; D004912:Erythrocytes / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D006801:Humans; D007118:Immunoassay; D007201:Indicator Dilution Techniques