Ribosomal ribonucleic acid (rRNA) gene typing for species identification.
Deoxyribonucleic acid (DNA) typing of ribosomal ribonucleic acid (rRNA) genes was performed with a polymerase chain reaction (PCR) assay for species identification. A variable region of the 28S ribosomal RNA gene was amplified with primers complementary to flanking sequences phylogenetically well conserved. The products of twelve animal DNAs (human, Japanese monkey, dog, cattle, pig, cat, rabbit, mouse, rat, chicken, frog, and fish) were separated by polyacrylamide gel electrophoresis, each revealing a few bands ranging from 150 to 100 base pairs. The band patterns obtained from each DNA sample differed in number and size, which indicates the applicability of the method to species identification. Samples containing either as little as 1 pg of DNA or degraded DNA of 0.2 to 0.5 kb in length were able to give detectable bands. Postmortem human tissue DNAs were tested as an example. They showed a pattern identical to the human control one, which was distinct from those of the other animals examined.
Naito
E
E
Department of Forensic Science, Niigata University School of Medicine, Japan.
Dewa
K
K
Ymanouchi
H
H
Kominami
R
R
D000818:Animals; D001483:Base Sequence; D002645:Chickens; D002871:Chromosome Banding; D004275:DNA, Ribosomal / Q000032:analysis* / Q000737:chemistry; D005399:Fishes; D005838:Genotype; D006801:Humans; D008322:Mammals / Q000145:classification* / Q000235:genetics; D008969:Molecular Sequence Data; D005810:Multigene Family; D016133:Polymerase Chain Reaction; D012339:RNA, Ribosomal, 28S / Q000737:chemistry / Q000235:genetics*; D011898:Ranidae; D013045:Species Specificity; D013698:Templates, Genetic; D014714:Vertebrates / Q000145:classification* / Q000235:genetics
D004275:DNA, Ribosomal; D012339:RNA, Ribosomal, 28S