[Gene amplification with PCR and sequence-specific HLA oligotyping].
Genetic polymorphism in the HLA-class II region has been identified by the analysis of the polymerase-chain reaction (PCR) products using sequence-specific oligonucleotide (SSO). The PCR-SSO method permits precise and direct analysis of allelic variations with as little as 1 microgram of genomic DNA. The standardized, uniform hybridization and critical wash protocol of the Eurotransplant-typing kit [2] enables HLA-typing independent of gene expression and lymphocytes quality. One of the advantages of this technique is that the definition of subtypes is much better than is typing by serology.
Holzberger
G
G
Institut f. Immunhämatologie und BSD, Hessen, Frankfurt/Main BRD.
Frank
R
R
Boehm
B O
BO
Seidl
S
S
D000595:Amino Acid Sequence / Q000235:genetics; D001483:Base Sequence / Q000235:genetics; D015342:DNA Probes; D005784:Gene Amplification / Q000235:genetics*; D006684:HLA-DR Antigens / Q000235:genetics; D059811:HLA-DRB1 Chains; D000949:Histocompatibility Antigens Class II / Q000235:genetics*; D006801:Humans; D008838:Microcomputers; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D010641:Phenotype; D016133:Polymerase Chain Reaction / Q000295:instrumentation*; D012815:Signal Processing, Computer-Assisted / Q000295:instrumentation*
D015342:DNA Probes; D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains; D000949:Histocompatibility Antigens Class II; D015345:Oligonucleotide Probes
Gen-Amplifikation mit der PCR und sequenzspezifische HLA-Oligotypisierung (SSO).