Gas chromatographic determination of 2,5-hexanedione in urine as an indicator of exposure to n-hexane.
A sensitive and specific method for determination of 2,5-hexanedione in urine is described. Treatment of the urine specimen directly with n-butylamine yields n-butyl 2,5-dimethyl pyrrole. The latter is extracted into diisopropylether and analyzed by gas chromatography (GC) with flame ionization detection (FID), thermionic specific detection (TSD) (N mode), or mass spectrometric detection (MS). The minimum detectable quantities are 1 mg/L urine when employing FID with a coefficient of variation of less than 6%. Recovery of 2,5-hexanedione added to the urine at the level of 10 mg/L was 78.9%.
Vohra
K
K
Occupational Health Laboratory, Ontario Ministry of Labour, Weston, Canada.
Gaind
V S
VS
D002849:Chromatography, Gas; D006586:Hexanes / Q000652:urine*; D006588:Hexanones / Q000652:urine*; D006801:Humans; D007202:Indicators and Reagents; D013058:Mass Spectrometry; D016273:Occupational Exposure
D006586:Hexanes; D006588:Hexanones; D007202:Indicators and Reagents; C026385:n-hexane; C011269:2,5-hexanedione