Localization of a 210-kDa microtubule-interacting protein in the yeast Saccharomyces cerevisiae.
Using the monoclonal antibody MA-01, which recognizes a 210-kDa protein in cell-free extracts, spindle and cytoplasmic microtubules were visualized in budding yeast, Saccharomyces cerevisiae. In additional, a spot-like staining was found beneath the plasma membrane, revealing in part correlation with F-actin distribution. This pattern was common for cells of all cell-cycle stages. The interaction of the protein recognized by MA-01 with microtubules was confirmed in the double labeling with a polyclonal antitubulin antibody and by the sensitivity of intranuclear structures stained by MA-01 to the microtubule disrupting drug nocodazole.
Hasek
J
J
Institute of Microbiology, Czechoslovak Academy of Sciences, Prague.
Jochová
J
J
Dráber
P
P
Viklický
V
V
Streiblová
E
E
D000199:Actins / Q000032:analysis; D000911:Antibodies, Monoclonal; D003593:Cytoplasm / Q000737:chemistry / Q000276:immunology; D005455:Fluorescent Antibody Technique; D008869:Microtubule-Associated Proteins / Q000276:immunology / Q000302:isolation & purification*; D008870:Microtubules / Q000737:chemistry / Q000276:immunology; D012441:Saccharomyces cerevisiae / Q000737:chemistry* / Q000276:immunology; D008941:Spindle Apparatus / Q000737:chemistry / Q000276:immunology
D000199:Actins; D000911:Antibodies, Monoclonal; D008869:Microtubule-Associated Proteins