Modification of the volumetric growth responses and steady-state hypoxic fractions of xenografted DLD-2 human colon carcinomas by administration of basic fibroblast growth factor or suramin.
We studied the growth characteristics and hypoxic fractions of DLD-2 human colon tumours xenografted into male nude mice either in the unperturbed state or after i.p. injection (q.i.d. x 7) of basic fibroblast growth factor (0.25 mg kg-1) or suramin (50 mg kg-1). Hypoxic fractions were measured by clonogenic excision assay 1 day after administration b FGF or suramin was stopped. As compared to controls, the growth of tumours in b FGF treated mice was increased by a factor of 1.5 as indicated by the relative volumes of tumours on the day of excision. Similarly, suramin decreased the growth of DLD-2 tumours by a factor of 1.6. The percentage of hypoxic cells in control neoplasms was 42.9% (95% confidence limits 34.2-52.1%). In mice that received basic fibroblast growth factor injections, hypoxic fractions decreased to 19.1% (95% confidence limits 13.5-26.9%). In contrast, in mice treated with suramin, the percentage of hypoxic cells increased to 74.0% (95% confidence limits 65.3-83.9%). These data indicate that the biology of solid tumours can be significantly modified by alteration of growth factor status.
Leith
J T
JT
Department of Radiation Medicine, Brown University School of Medicine, Providence, RI 02912.
Papa
G
G
Quaranto
L
L
Michelson
S
S
D000230:Adenocarcinoma / Q000473:pathology* / Q000503:physiopathology; D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D003110:Colonic Neoplasms / Q000473:pathology* / Q000503:physiopathology; D004305:Dose-Response Relationship, Drug; D004307:Dose-Response Relationship, Radiation; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D006801:Humans; D000860:Hypoxia; D008297:Male; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D013498:Suramin / Q000494:pharmacology*; D014183:Transplantation, Heterologous; D014965:X-Rays
D016222:Fibroblast Growth Factor 2; D013498:Suramin