Cytoplasmic localization of the DNA virus frog erythrocytic virus.
In situ hybridization, using a biotinylated clone of frog erythrocytic virus (FEV), was conducted to determine the location of viral sequences in bullfrog erythrocytes. FEV-specific hybridization signals were found to correspond to mature cytoplasmic viral particles and assembly sites. These data are consistent with electron microscopic observations of viral assembly in the erythrocyte cytoplasm. Although FEV has morphological and biochemical properties similar to frog virus 3, our data suggest that the site of DNA replication and assembly of FEV is more similar to that of the poxviruses.
Gruia-Gray
J
J
Laboratory of Cellular and Developmental Biology, NIDDK, National Institutes of Health, Bethesda, MD 20892.
Ringuette
M
M
Desser
S S
SS
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003593:Cytoplasm / Q000382:microbiology*; D004261:DNA Replication / Q000235:genetics / Q000502:physiology; D004267:DNA Viruses / Q000235:genetics* / Q000502:physiology; D004279:DNA, Viral / Q000235:genetics*; D004912:Erythrocytes / Q000382:microbiology*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D011898:Ranidae / Q000382:microbiology*; D014779:Virus Replication / Q000235:genetics / Q000502:physiology
D004279:DNA, Viral