title: Prefabrication of free flaps using the omentum in rats.
abstract: The omentum used as a vascular carrier is a new experimental model for prefabrication of free flaps in the rat. A 2.5 x 4 cm patch of omentum with gastroepiploic vessels and its rich vascular arcades was transferred under a bipedicled 2.5 x 6 cm right abdominal panniculocutaneous flap. At subsequent time intervals (postoperative days 0, 3, 5, 7, and 14), the skin pedicles were divided and the skin flap was raised as a composite island flap vascularized only by the underlying omental patch. The composite flap was then sutured back in place. Prefabricated flaps examined postoperatively demonstrated a dye fluorescence index (DFI) of 4.60% +/- 2.82% and 1.67% +/- 4.08% flap survival (FS) in the day 0 group; in the day 3 group, 9.83% +/- 1.47% of DFI and 15.00% +/- 10.35% of FS; in the day 5 group, 28.39% +/- 5.25% of DFI and 86.0% +/- 12.74% of FS (P less than 0.05); in the day 7 group, 38.19% +/- 7.52% of DFI and 98.13% +/- 3.72% of FS (P less than 0.05); and, in the day 14 group, 49.55% +/- 6.70% of DFI and 100% of FS (P less than 0.05). India-ink injection and histologic examination confirmed revascularization of the overlying skin by day 5.
authors.affiliation: Microsurgical Research Laboratory, New York University Medical Center, New York.
authors.forename: L
authors.initials: L
authors.lastname: Zhang
authors.forename: R E
authors.initials: RE
authors.lastname: Tuchler
authors.forename: B
authors.initials: B
authors.lastname: Chang
authors.forename: N
authors.initials: N
authors.lastname: Bakshandeh
authors.forename: W W
authors.initials: WW
authors.lastname: Shaw
authors.forename: J W
authors.initials: JW
authors.lastname: Siebert
mesh_terms: D000818:Animals; D002244:Carbon; D004396:Coloring Agents; D005452:Fluoresceins; D006085:Graft Survival; D008297:Male; D009336:Necrosis; D009852:Omentum / Q000098:blood supply / Q000473:pathology / Q000637:transplantation*; D051381:Rats; D011919:Rats, Inbred Strains; D012867:Skin / Q000098:blood supply / Q000473:pathology; D016038:Skin Transplantation / Q000379:methods* / Q000473:pathology; D013524:Surgical Flaps / Q000379:methods* / Q000473:pathology
chemical_list: D004396:Coloring Agents; D005452:Fluoresceins; C028433:chinese ink; D002244:Carbon