Cortical norepinephrine release elicited in situ by N-methyl-D-aspartate (NMDA) receptor stimulation: a microdialysis study.
Norepinephrine (NE) release measured by microdialysis from probes positioned in the prefrontal cortex of anesthetized rats was increased when N-methyl-D-aspartate (NMDA) was contained in the microdialysis medium. NE release increased by a factor of 3.6 compared to prior baseline levels when 1 mM NMDA was applied for 30 min. This increase was largely reversible, and when a second stimulation was applied using aconitine (0.3 mM, 30 min), which acts on voltage-sensitive sodium channels, a second evoked release of NE was observed, of a similar magnitude as that evoked by NMDA. Dizocilpine (300 nM), which blocks cation channels associated with NMDA receptors, prevented the NMDA-elicited NE increase but not the aconitine-elicited increase.
Lehmann
J
J
Department of Mental Health Sciences, Hahnemann University, Philadelphia, PA 19102-1192.
Valentino
R
R
Robine
V
V
D000157:Aconitine / Q000494:pharmacology*; D000818:Animals; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D003956:Dialysis / Q000379:methods; D016291:Dizocilpine Maleate / Q000494:pharmacology*; D007700:Kinetics; D008297:Male; D016202:N-Methylaspartate / Q000008:administration & dosage / Q000494:pharmacology*; D009638:Norepinephrine / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D016194:Receptors, N-Methyl-D-Aspartate / Q000187:drug effects / Q000502:physiology*; D013997:Time Factors
D016194:Receptors, N-Methyl-D-Aspartate; D016202:N-Methylaspartate; D016291:Dizocilpine Maleate; D009638:Norepinephrine; D000157:Aconitine