title: Collagen type II in Langer-Saldino achondrogenesis: absence of major abnormalities in a less severe case.
abstract: Collagen extracted either from cartilage or synthesized in vitro was analyzed to identify possible molecular defects in the cartilaginous matrix of a male fetus suffering from a mild form of type II achondrogenesis (Langer-Saldino). The tissue architecture of the patient's cartilage was markedly altered and showed numerous fibrous vascular canals which were focally stained by antibodies against collagens I and III. Collagen II was present, although heterogenously distributed throughout the cartilaginous matrix. Upon electrophoretic separation, however, the patient's femoral head cartilage showed the presence of collagens II, IX and XI only, which was similar to an age-matched control. The hydroxyproline/hydroxylysine ratio of collagen II of the patient was not significantly different from that of the control. Likewise, the compositions of collagens synthesized by cultured chondrocytes as well as fibroblasts were similar in the patient and the control. The results provide strong evidence that, in the present mild case of Langer-Saldino achondrogenesis, collagen II is expressed and regularly hydroxylated at its lysyl residues. This may indicate that cartilage components other than collagen II may be responsible for the altered tissue organization observed. Along with previous observations, our data suggest that the degree of biochemical matrix alterations may be related to the severity of the clinical phenotype.
authors.affiliation: Institut für Medizinische Molekularbiologie, Medizinische Universität, Lübeck, Germany.
authors.forename: B
authors.initials: B
authors.lastname: Bätge
authors.forename: A
authors.initials: A
authors.lastname: Nerlich
authors.forename: R
authors.initials: R
authors.lastname: Brenner
authors.forename: C
authors.initials: C
authors.lastname: Yang
authors.forename: P K
authors.initials: PK
authors.lastname: Müller
mesh_terms: D000130:Achondroplasia / Q000175:diagnosis / Q000378:metabolism*; D002356:Cartilage / Q000737:chemistry / Q000378:metabolism*; D003094:Collagen / Q000096:biosynthesis* / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005260:Female; D005270:Femur Head / Q000737:chemistry / Q000378:metabolism*; D005315:Fetal Diseases / Q000175:diagnosis / Q000378:metabolism*; D005333:Fetus / Q000378:metabolism*; D006801:Humans; D015151:Immunoblotting / Q000379:methods; D007150:Immunohistochemistry / Q000379:methods; D066298:In Vitro Techniques; D007231:Infant, Newborn; D008297:Male; D011247:Pregnancy; D012720:Severity of Illness Index
chemical_list: D003094:Collagen