Lanthanide ion luminescence as a probe of DNA structure. 2. Non- guanine-containing oligomers and nucleotides.
Oligo(dC)8, oligo(dA)8, and oligo(dT)8 as well as d-CMP, d-AMP, and d-TMP, when complexed to Eu(3+), possess two classes of Eu(3+) binding environment. The binding environments consist of two classes, tight sites which coordinate two H2O molecules, and weaker sites which coordinate six or seven, analogous to the previously studied guanine-containing molecules. It is inferred that the tight class of Eu(3+) ion site observed with these oligomers and nucleotides corresponds to dimeric or polymeric structures. Comparison of the results for the guanine and non-guanine containing oligomers suggests that Eu(3+) possibly coordinates base nitrogen atoms in the former and in an outer sphere mode (hydrogen bonding via the H2O molecules coordinated to Eu(3+)) in the species examined here.
Klakamp
S L
SL
Department of Chemistry, Pennsylvania State University, University Park.
Horrocks
W D
WD
D001665:Binding Sites; D004247:DNA / Q000737:chemistry*; D003838:Deoxyadenine Nucleotides / Q000737:chemistry; D003843:Deoxycytidine Monophosphate / Q000737:chemistry; D005063:Europium / Q000737:chemistry*; D006860:Hydrogen Bonding; D008163:Luminescent Measurements; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D013057:Spectrum Analysis; D013938:Thymidine Monophosphate / Q000737:chemistry; D014867:Water / Q000737:chemistry
D003838:Deoxyadenine Nucleotides; D009838:Oligodeoxyribonucleotides; C027903:oligo (dT); C030570:oligodeoxyadenylic acid; C033539:oligodeoxycytidylic acid; D014867:Water; D003843:Deoxycytidine Monophosphate; D013938:Thymidine Monophosphate; D005063:Europium; C012873:2'-deoxy-5'-adenosine monophosphate; D004247:DNA