title: Association of titin and myosin heavy chain in developing skeletal muscle.
abstract: To understand molecular interactions that organize developing myofibrils, we examined the biosynthesis and interaction of titin and myosin heavy chain in cultures of developing muscle. Use of pulse-labeling, immunoprecipitation, and a reversible cross-linking procedure demonstrates that within minutes of synthesis, titin and myosin heavy chain can be chemically cross-linked into very large, detergent-resistant complexes retaining many features of intact myotubes. These complexes, predominantly of titin and myosin, occur very early in myofibrillogenesis as well as later. These data suggest that synthesis and assembly of titin and myosin are temporally and spatially coordinated in nascent myofibrils and support the hypothesis that titin molecules help to organize sarcomere formation.
authors.affiliation: Department of Biochemistry, University of Iowa, Iowa City 52242.
authors.forename: W B
authors.initials: WB
authors.lastname: Isaacs
authors.forename: I S
authors.initials: IS
authors.lastname: Kim
authors.forename: A
authors.initials: A
authors.lastname: Struve
authors.forename: A B
authors.initials: AB
authors.lastname: Fulton
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D002352:Carrier Proteins / Q000378:metabolism; D002478:Cells, Cultured; D002642:Chick Embryo; D064211:Connectin; D003432:Cross-Linking Reagents / Q000494:pharmacology; D003599:Cytoskeleton / Q000187:drug effects / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005455:Fluorescent Antibody Technique; D007074:Immunoglobulin G; D008715:Methionine / Q000378:metabolism; D009124:Muscle Proteins / Q000302:isolation & purification / Q000378:metabolism*; D009132:Muscles / Q000166:cytology / Q000196:embryology / Q000378:metabolism*; D009218:Myosins / Q000302:isolation & purification / Q000378:metabolism*; D011494:Protein Kinases; D013388:Succinimides / Q000494:pharmacology; D013462:Sulfur Radioisotopes
chemical_list: D000911:Antibodies, Monoclonal; D002352:Carrier Proteins; D064211:Connectin; D003432:Cross-Linking Reagents; D007074:Immunoglobulin G; D009124:Muscle Proteins; D013388:Succinimides; D013462:Sulfur Radioisotopes; C028722:ethylene glycolylbis(succinimidyl succinate); D008715:Methionine; D011494:Protein Kinases; D009218:Myosins