title: Cloning of Candida boidinii DNA fragments promoting autonomous replication of plasmids in Saccharomyces cerevisiae.
abstract: Fragments of Candida boidinii chromosomal DNA were inserted into the integrative vector YIp-kanr and examined for the presence of sequences promoting autonomous replication of plasmids in Saccharomyces cerevisiae. Restriction maps of two plasmids, designated S6/4 and S6/5, originating from the same S. cerevisiae transformant, were constructed. Southern hybridization data confirmed that the plasmids carry sequences from the C. boidinii chromosome. Both plasmids transform S. cerevisiae strains at 4-5-fold higher frequency than cloning vectors based on the replication origin of the 2 microns plasmid. Mitotic stability of the constructed plasmids is similar to that of the 2 mu-based vector pNF2 in S. cerevisiae.
authors.affiliation: Institute of Microbiology, Czechoslovak Academy of Sciences, Prague.
authors.forename: I
authors.initials: I
authors.lastname: Janatová
authors.forename: O
authors.initials: O
authors.lastname: Navrátil
mesh_terms: D015139:Blotting, Southern; D002175:Candida / Q000235:genetics*; D003001:Cloning, Molecular; D004261:DNA Replication; D004271:DNA, Fungal; D010957:Plasmids / Q000235:genetics*; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D014170:Transformation, Genetic
chemical_list: D004271:DNA, Fungal