title: Protein kinase activity and synaptic plasticity in a chick brain slice.
abstract: In an in vitro slice preparation of the chick brain it is possible to record responses to single electrical stimuli from within the intermediate and medial part of the hyperstriatum ventrale (IMHV), a region known to be involved in learning. The amplitude of such responses is significantly increased by superfusion of the slice with 10 microM 4 beta-phorbol 12,13 diacetate (PDAc), a phorbol ester which stimulates protein kinase activity. The ability of PDAc to induce potentiation is greatest in chicks less than 6 days old. Administration of the kinase antagonist H7 prevents the induction of persistent potentiation and in fact produces a long lasting depression of response amplitude. H7 also produces a short term increase in excitability within the IMHV and results in increased expression of N-methyl-D-aspartate receptor activity.
authors.affiliation: Division of Neurobiology, Medical School, University of Newcastle upon Tyne, UK.
authors.forename: P M
authors.initials: PM
authors.lastname: Bradley
authors.forename: B D
authors.initials: BD
authors.lastname: Burns
authors.forename: J
authors.initials: J
authors.lastname: Titmuss
authors.forename: A C
authors.initials: AC
authors.lastname: Webb
mesh_terms: D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D000375:Aging / Q000502:physiology; D000818:Animals; D002645:Chickens; D005071:Evoked Potentials / Q000502:physiology; D066298:In Vitro Techniques; D007546:Isoquinolines / Q000494:pharmacology; D009473:Neuronal Plasticity / Q000502:physiology*; D010703:Phorbol Esters / Q000494:pharmacology; D010879:Piperazines / Q000494:pharmacology; D016548:Prosencephalon / Q000201:enzymology*; D011493:Protein Kinase C / Q000037:antagonists & inhibitors / Q000378:metabolism*; D013569:Synapses / Q000201:enzymology*
chemical_list: D007546:Isoquinolines; D010703:Phorbol Esters; D010879:Piperazines; C008874:phorbol-12,13-diacetate; D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D011493:Protein Kinase C