Fructose-1,6-bisphosphate does not ameliorate hypoxic ischemic injury to the central nervous system in the newborn pig.
METHODS
Fructose-1,6-bisphosphate has been shown to improve the outcome of hypoxic ischemic brain injury in adult rabbits. We wished to see if these results could be extended to a newborn animal. Twenty-four 0- to 3-day-old piglets were randomized to receive 300 mg/kg of fructose-1,6-bisphosphate 5 mins before injury, followed by a continuous infusion of 15 mg/kg/min of fructose-1,6-bisphosphate for the next 90 mins, or the equivalent volume of normal saline. Hypoxic ischemic central nervous system damage was induced by ligating both carotid arteries and reducing their BP to two thirds of the normal value for 30 mins. In the last 15 mins of this 30 mins, the FIO2 was reduced to 0.6. At 30 mins, the piglets were resuscitated with an FIO2 of 1.0, the carotid ligatures were released, and the removed blood was reinfused.


RESULTS
The neurologic examination scores at 1, 2, and 3 days after injury and pathologic examination scores at 3 days after injury were not different in the fructose-1,6-bisphosphate-treated and the control animals.


CONCLUSIONS
Fructose-1,6-bisphosphate does not ameliorate hypoxic ischemic brain injury in the newborn pig.
LeBlanc
M H
MH
Department of Pediatrics, University of Mississippi Medical Center, Jackson 39216-4505.
Parker
C C
CC
Vig
V
V
Smith
E E
EE
Brown
E G
EG
D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000473:pathology; D002545:Brain Ischemia / Q000097:blood / Q000188:drug therapy* / Q000473:pathology; D004195:Disease Models, Animal; D004353:Drug Evaluation, Preclinical; D005635:Fructosediphosphates / Q000008:administration & dosage* / Q000097:blood; D002534:Hypoxia, Brain / Q000097:blood / Q000188:drug therapy* / Q000473:pathology; D013552:Swine; D013553:Swine Diseases / Q000097:blood / Q000188:drug therapy* / Q000473:pathology; D013997:Time Factors
D005635:Fructosediphosphates; C029063:fructose-1,6-diphosphate