Conformation ability test of human, rabbit and bovine plasminogens and their specific interaction with streptokinase.
Human, rabbit and bovine plasminogens, having different sensitivity to streptokinase-activating action, differ, according to spectrophotometric titration, tryptophan fluorescence and circular dichroism spectroscopy, in the state of tyrosine and tryptophan residues, and in secondary and tertiary structures. Human plasminogen-streptokinase equimolar complex formation (according to gel chromatography) is accompanied by a differential ultraviolet spectrum. Difference spectroscopy is a convenient and adequate means of studying the formation of the said complexes. Streptokinase-human plasminogen complex formation is not hindered by partial substitution of water (20%) with ethanol or dimethylsulphoxide or by addition of 0.001 M sodium dodecylsulphate. The complex is not formed in 6 M urea, in solution, at pH less than 2.0 or approximately 12.0-13.0, or with bovine plasminogen. Circular dichroism and tryptophan fluorescence spectral pattern changes during streptokinase-plasminogen complex formation enable us to conclude that streptokinase secondary and tertiary structures undergo certain rearrangements in the framework of the complex, while tryptophan-containing sites of the molecule are not drastically changed. The data obtained enable us to presuppose formation of streptokinase-rabbit plasminogen complexes which differ from human plasminogen complexes with streptokinase.
Nikandrov
V N
VN
Laboratory of Biochemistry, Byelorussian Research Institute of Epidemiology and Microbiology, Minsk.
Vorobyova
G V
GV
Yankovskaya
G S
GS
Demidchik
N V
NV
D000818:Animals; D002417:Cattle; D002942:Circular Dichroism; D004789:Enzyme Activation; D006801:Humans; D006863:Hydrogen-Ion Concentration; D010958:Plasminogen / Q000737:chemistry* / Q000378:metabolism; D011487:Protein Conformation; D011817:Rabbits; D013300:Streptokinase / Q000378:metabolism*; D014364:Tryptophan / Q000737:chemistry; D014443:Tyrosine / Q000737:chemistry
D014443:Tyrosine; D014364:Tryptophan; D010958:Plasminogen; D013300:Streptokinase