Use of [3H]-GBR12935 to measure dopaminergic nerve terminal growth into the developing rat striatum.
In this study we determined the temporal association between the appearance of the dopamine transporter, measured by 1-[2-(diphenyl-methoxy)ethyl]-4-(3- phenylpropyl)-piperazine ([3H]-GBR12935), a potent and selective inhibitor of dopamine uptake, and other biochemical markers of dopaminergic nerve terminal growth into the developing striatum. [3H]-GBR12935 binding was minimally detected in the rudimentary striatum of embryonic day 14 rat brains, increased to 23% of the adult level by birth, and reached the adult level during the fifth postnatal week. This finding contrasts with a slower developmental increase in [3H]-dopamine uptake, a functional measure of the transporter. Tyrosine hydroxylase activity levels followed a developmental curve similar to that of [3H]-GBR12935 binding but did not reach adult levels until the 7th postnatal week. Dopamine content increased at a slower rate, being only 10% and 92% of the adult level at birth and postnatal week 8, respectively. These results indicate that the appearance of a structural, but not optimally functional, dopamine transporter may be the earliest detectable biochemical index of dopaminergic nerve terminal growth into the striatum during development.
Le
W D
WD
Department of Neurology, Baylor College of Medicine, Houston, TX 77030.
Bostwick
J R
JR
Appel
S H
SH
D000375:Aging / Q000502:physiology*; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D003342:Corpus Striatum / Q000196:embryology* / Q000254:growth & development* / Q000378:metabolism; D004298:Dopamine / Q000378:metabolism*; D005314:Embryonic and Fetal Development; D005865:Gestational Age; D007980:Levodopa / Q000378:metabolism; D008297:Male; D009411:Nerve Endings / Q000378:metabolism / Q000502:physiology*; D010879:Piperazines / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D014316:Tritium
D010879:Piperazines; D014316:Tritium; D007980:Levodopa; C044630:1-(2 (diphenylmethoxy)ethyl)-4-(3-phenylpropyl)piperazine; D004298:Dopamine