title: Neutrophil and lymphocyte response to supplementation with vitamins C and E in young calves.
abstract: Calves, beginning at 3 d of age, were bottle-fed milk replacer unsupplemented, or supplemented with 10 g of vitamin C, or supplemented with 10 g of vitamin C plus 57 IU of vitamin E/kg of dry milk replacer (10 calves per treatment). Neutrophils from wk 2 and 4 blood samples were assayed for Staphylococcus aureus phagocytosis and neutrophil-mediated, antibody-dependent cellular cytotoxicity, and lymphocytes from those samples were assayed for concanavalin A-induced, phytohemagglutinin-induced, or pokeweed mitogen-induced proliferation. Ocular and nasal discharges of calves supplemented with vitamins C or C and E were less than those of controls for wk 1 to 8. Calves receiving supplementation with vitamin C and E tended to have higher mitogen-induced lymphocyte proliferative responses at wk 2. Neutrophil-mediated phagocytosis and antibody-dependent cellular cytotoxicity were lower at wk 2 and 4 for calves supplemented with vitamin C than for controls. Neutrophil function of calves supplemented with vitamins C and E together was near, or slightly higher than, that of unsupplemented calves for wk 2 and 4, suggesting that the addition of vitamin E negated the adverse effects that vitamin C alone had on neutrophil functions.
authors.affiliation: Department of Animal Sciences and Industry, Kansas State University, Manhattan 66506.
authors.forename: S D
authors.initials: SD
authors.lastname: Eicher-Pruiett
authors.forename: J L
authors.initials: JL
authors.lastname: Morrill
authors.forename: F
authors.initials: F
authors.lastname: Blecha
authors.forename: J J
authors.initials: JJ
authors.lastname: Higgins
authors.forename: N V
authors.initials: NV
authors.lastname: Anderson
authors.forename: P G
authors.initials: PG
authors.lastname: Reddy
mesh_terms: D000818:Animals; D000920:Antibody-Dependent Cell Cytotoxicity / Q000187:drug effects; D001205:Ascorbic Acid / Q000097:blood / Q000494:pharmacology*; D002417:Cattle / Q000254:growth & development / Q000276:immunology*; D007111:Immunity, Cellular / Q000187:drug effects; D008213:Lymphocyte Activation / Q000187:drug effects; D008214:Lymphocytes / Q000187:drug effects* / Q000276:immunology; D008297:Male; D009504:Neutrophils / Q000187:drug effects* / Q000276:immunology; D014810:Vitamin E / Q000097:blood / Q000494:pharmacology*
chemical_list: D014810:Vitamin E; D001205:Ascorbic Acid