title: Chemical modification of arginyl residues in silk fibroin: 1. Reaction of 1,2-cyclohexanedione in borate buffer.
abstract: Chemical modifications of silk fibroin were attempted in order to add new properties and functions to silk fibroin. The arginyl residue in solubilized silk fibroin was chemically modified with the reaction of 1,2-cyclohexanedione in borate buffer. FT-i.r. and c.d. spectra of the silk fibroin before and after the modification indicated that the fraction of random coil conformation increased with the modification. The chemical stability of the modified silk fibroin membrane was investigated in vitro with phosphate buffer. The modified arginyl residue in the membrane was considerably regenerated with the treatment in phosphate buffer.
authors.affiliation: National Institute of Sericultural and Entomological Science, Ibaraki, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Gotoh
authors.forename: M
authors.initials: M
authors.lastname: Tsukada
authors.forename: N
authors.initials: N
authors.lastname: Minoura
mesh_terms: D000818:Animals; D001120:Arginine / Q000737:chemistry*; D012831:Bombyx; D001881:Borates / Q000737:chemistry*; D002021:Buffers; D003512:Cyclohexanones / Q000737:chemistry*; D005349:Fibroins / Q000737:chemistry*; D005583:Fourier Analysis; D007700:Kinetics
chemical_list: D001881:Borates; D002021:Buffers; D003512:Cyclohexanones; C018031:1,2-cyclohexanedione; D005349:Fibroins; D001120:Arginine