Recent developments in quantitative affinity chromatography.
This review surveys developments during the past decade in the use of quantitative affinity chromatography as a means of evaluating equilibrium constants for solute-ligand and solute-matrix interactions. Topics include allowance for multivalency of the partitioning solute, removal of the myth that highly substituted affinity matrices are unsuitable for zonal quantitative affinity chromatography, adaptation of the technique to allow characterization of high-affinity interactions and the application of quantitative affinity chromatography theory to the characterization of biospecific adsorption phenomena in cellular systems.
Winzor
D J
DJ
Department of Biochemistry, University of Queensland, Brisbane, Australia.
D000818:Animals; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002846:Chromatography, Affinity; D005634:Fructose-Bisphosphate Aldolase / Q000378:metabolism; D006801:Humans; D008024:Ligands; D009210:Myofibrils / Q000201:enzymology
D008024:Ligands; D005634:Fructose-Bisphosphate Aldolase