Drug residue analysis using immunoaffinity chromatography.
The background and applicability of immunoaffinity chromatographic separations and clean-up to drug residue analysis of agricultural commodities is discussed. The uses of antibody specificity for separation and concentration of drug residues are presented. Examples of immunoaffinity chromatography for the determination of residues of (1) nortestosterone and methyl testosterone in swine muscle, urine and bile; (2) chloramphenicol in swine tissue, eggs and milk; (3) clenbuterol in calf urine; (4) zeranol and beta-zearalanolin in calf urine: (5) diethylstilbestrol, dienestrol and hexestrol in calf urine are presented. Further, examples of the successful coupling of immunoaffinity separations with other chromatographic techniques such as gas chromatography and high-performance liquid chromatography are presented.
Katz
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Department of Biochemistry and Microbiology, Cook College, NJAES Rutgers, State University of New Jersey, New Brunswick 08903-0231.
Siewierski
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D000818:Animals; D002417:Cattle; D002846:Chromatography, Affinity / Q000379:methods*; D004350:Drug Residues / Q000032:analysis*; D005504:Food Analysis; D007118:Immunoassay / Q000379:methods*; D013552:Swine