Sequential inactivation of ammonium and glucose transport in Saccharomyces cerevisiae during fermentation.
Ethanol at concentrations above 12% (v/v) in mineral medium with glucose and with ammonium as the only nitrogen source induced rapid inactivation of the ammonium transport system in the strain IGC 3507 of Saccharomyces cerevisiae terminating protein synthesis. Subsequently, when glucose was present, the glucose transport system was irreversibly inactivated. This two-step mechanism may play a decisive role when ethanol stops fermentation by S. cerevisiae, before all the fermentable sugar has been consumed.
Cardoso
H
H
Laboratory of Biology, University of Minho, Braga, Portugal.
Leão
C
C
D001692:Biological Transport / Q000187:drug effects; D003470:Culture Media; D000431:Ethanol / Q000494:pharmacology; D005285:Fermentation / Q000187:drug effects; D005947:Glucose / Q000378:metabolism*; D000644:Quaternary Ammonium Compounds / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000254:growth & development / Q000378:metabolism*
D003470:Culture Media; D000644:Quaternary Ammonium Compounds; D000431:Ethanol; D005947:Glucose