title: Sequences from a family of bovine Y-chromosomal repeats.
abstract: A 307-bp Sau3AI fragment previously cloned by deletion enrichment from the bovine Y chromosome was used to isolate a larger lambda EMBL3A genomic cattle clone. The whole 13-kb insert did not give a sex-specific pattern of hybridization to Southern blots of cattle DNA. Subclones from this phage, however, did show that this fragment had a Y-chromosomal origin. It was estimated that at least 40% of the cattle Y chromosome is composed of repeated sequences related to those within these subcloned fragments. Sequences within these subclones are male-specific or male-enriched also in sheep, goats, and deer. Comparison of cattle and sheep homologues of these sequences reveals that much greater amplification and rearrangement have occurred on the cattle Y chromosome than on the sheep Y. The apparent insertion of sequences into cattle Y-specific sequences relative to the sheep homologues suggests possible mechanisms for the evolution of the artiodactyl Y chromosome.
authors.affiliation: Victorian Institute of Animal Science, Department of Agriculture, Australia.
authors.forename: M E
authors.initials: ME
authors.lastname: Matthews
authors.forename: K C
authors.initials: KC
authors.lastname: Reed
mesh_terms: D000818:Animals; D015139:Blotting, Southern; D002417:Cattle / Q000235:genetics*; D003001:Cloning, Molecular; D004247:DNA; D003670:Deer / Q000235:genetics; D005260:Female; D006041:Goats / Q000235:genetics; D008297:Male; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D012756:Sheep / Q000235:genetics; D013045:Species Specificity; D014998:Y Chromosome
chemical_list: D004247:DNA