title: Effective method for synthetic peptide immobilization that increases the sensitivity and specificity of ELISA procedures.
abstract: A procedure is described for the immobilization of synthetic peptide antigens on a plastic solid phase for performing ELISA. The use of a streptavidin-biotinylated peptide system for coating microplates with peptide antigen markedly increased both the sensitivity and the specificity compared to a standard ELISA based on synthetic peptides. The procedure was used for the detection of HIV-1-specific antibodies.
authors.affiliation: Shemyakin Institute of Bioorganic Chemistry, Academy of Sciences of Russia, Moscow.
authors.forename: V S
authors.initials: VS
authors.lastname: Ivanov
authors.forename: Z K
authors.initials: ZK
authors.lastname: Suvorova
authors.forename: L D
authors.initials: LD
authors.lastname: Tchikin
authors.forename: A T
authors.initials: AT
authors.lastname: Kozhich
authors.forename: V T
authors.initials: VT
authors.lastname: Ivanov
mesh_terms: D000595:Amino Acid Sequence; D004797:Enzyme-Linked Immunosorbent Assay; D015483:HIV Antibodies / Q000032:analysis; D006679:HIV Seropositivity / Q000276:immunology; D006801:Humans; D008969:Molecular Sequence Data
chemical_list: D015483:HIV Antibodies