title: Nucleotide sequence and structural relationships of a chloramphenicol acetyltransferase encoded by the plasmid pSCS6 from Staphylococcus aureus.
abstract: The 4.6 kb chloramphenicol resistance (Cm) plasmid, pSCS6, isolated from a naturally occurring Staphylococcus aureus biotype C encoded an inducible chloramphenicol acetyltransferase (CAT). The respective cat gene and its regulatory region were cloned. Sequence analyses revealed two open reading frames: one for a 9-amino acid leader peptide and the other for the 215-amino acid CAT monomer. Comparisons of the predicted CAT amino acid sequences revealed a high degree of similarity between CAT from pSCS6 and the CAT variants encoded by Cm plasmids of the pC221 family. These close structural relationships suggested an intraspecific exchange of Cm-determinants between Staph. aureus of human and bovine biotype.
authors.affiliation: Institut für Bakteriologie und Immunologie, Justus-Liebig-Universität Giessen, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Cardoso
authors.forename: S
authors.initials: S
authors.lastname: Schwarz
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D008969:Molecular Sequence Data; D010957:Plasmids; D013211:Staphylococcus aureus / Q000201:enzymology / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial; D015500:Chloramphenicol O-Acetyltransferase