High-performance liquid chromatography determination of polydeoxyribonucleotides in plasma: its application to the determination of defibrotide's pharmacokinetics in the rabbit.
We describe an HPLC method for the determination of whole polydeoxyribonucleotides in animal plasma. This method was compared to a colorimetric method, which evaluates the sugar moiety of polydeoxyribonucleotides, and to an agarose gel electrophoresis method, which evaluates the whole polydeoxyribonucleotides as does the HPLC method, and was found to give results very close to those obtained with these two other methods. A pharmacokinetic study of the antithrombotic, profibrinolytic, polydeoxyribonucleotidic drug defibrotide was carried out by evaluating the plasma drug levels by these three methods. The pharmacokinetic parameters calculated from the data are very similar.
Porta
R
R
CRINOS Industria Farmacobiologica S.p.A., Villa Guardia, Italy.
Moltrasio
D
D
Pescador
R
R
Lanzarotti
E
E
Mantovani
M
M
Prino
G
G
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003124:Colorimetry; D004587:Electrophoresis, Agar Gel; D005069:Evaluation Studies as Topic; D005343:Fibrinolytic Agents / Q000097:blood / Q000493:pharmacokinetics; D008297:Male; D011089:Polydeoxyribonucleotides / Q000097:blood* / Q000493:pharmacokinetics; D011817:Rabbits
D005343:Fibrinolytic Agents; D011089:Polydeoxyribonucleotides; C036901:defibrotide