Pulsed-field gel electrophoresis for epidemiologic studies of Campylobacter hyointestinalis isolates.
Campylobacter hyointestinalis was isolated from five members of the same family who had previously consumed raw milk. Pulsed-field gel electrophoresis of genomic DNAs from the five strains, after digestion with restriction endonuclease SalI, revealed that three strains had identical genome patterns and therefore appeared to be related, whereas the other two had completely different genome patterns and appeared to be unrelated. We report here for the first time the isolation of C. hyointestinalis from family members who had consumed raw milk. Our study also demonstrates the usefulness of pulsed-field gel electrophoresis for epidemiologic studies of this unusual campylobacter.
Salama
S M
SM
Department of Medical Microbiology and Infectious Diseases, University of Alberta, Edmonton, Canada.
Tabor
H
H
Richter
M
M
Taylor
D E
DE
D001431:Bacteriological Techniques; D002167:Campylobacter / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D002169:Campylobacter Infections / Q000453:epidemiology / Q000382:microbiology; D004269:DNA, Bacterial / Q000235:genetics*; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods*; D005069:Evaluation Studies as Topic; D005260:Female; D006801:Humans; D007223:Infant; D013045:Species Specificity
D004269:DNA, Bacterial