title: [Expression of human urokinase in inoculated rodent cells. Loss of introns during gene transfer using a retroviral vector].
abstract: Expression of urokinase in murine and rat cells was performed by two recombinant constructs, one containing cDNA and the other--hybrid (cDNA/genome) variant of human urokinase gene conserving 7 introns of 10, in the eukaryotic retrovirus vector pPS-3-neo. DNA of both constructs was introduced into packaging cell line psi 2 by a standard Ca-phosphate transfection technique. Infection of mouse and rat fibroblasts BALB/c 3T3 and Rat I with virus particles, produced by transfected psi 2 cells, led to an integration into the host genome of one or two recombinant proviral copies. Stable expression and secretion into the culture medium of glycosylated high molecular weight human urokinase was observed for both cell types. For the hybrid gene construct, precise excision of intervening sequences was shown during transferring of genetic material from packaging to recipient cells.
authors.forename: A Ia
authors.initials: AIa
authors.lastname: Shevelev
authors.forename: R V
authors.initials: RV
authors.lastname: Kondratov
authors.forename: I N
authors.initials: IN
authors.lastname: Rybalkin
authors.forename: V S
authors.initials: VS
authors.lastname: Prasolov
mesh_terms: D016475:3T3 Cells; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D015153:Blotting, Western; D004247:DNA; D005347:Fibroblasts / Q000382:microbiology; D015870:Gene Expression; D005814:Genes, Viral; D006801:Humans; D007438:Introns; D051379:Mice; D008969:Molecular Sequence Data; D010957:Plasmids; D051381:Rats; D011994:Recombinant Proteins / Q000235:genetics; D012190:Retroviridae / Q000235:genetics*; D014162:Transfection; D014568:Urokinase-Type Plasminogen Activator / Q000235:genetics*; D014771:Virion
chemical_list: D011994:Recombinant Proteins; D004247:DNA; D014568:Urokinase-Type Plasminogen Activator
vernacular_title: Ekspressiia urokinazy cheloveka v perevivaemykh kletkakh gryzunov. Utrata intronov v khode perenosa gena s pomoshch'iu retrovirusnogo vektora.