title: Effect of growth rate on ethanol tolerance of Saccharomyces cerevisiae.
abstract: delta 5,7-Sterol-accumulating Saccharomyces cerevisiae cells growing in chemostat at a specific growth rate of 0.075/h exhibited higher ethanol tolerance measured as ethanol-induced death and anaerobic growth inhibition than the cells growing at 0.2/h, the difference being dependent on the carbon-to-nitrogen molar proportion in the medium. The observed difference in sensitivity to ethanol of anaerobic growth between the slowly and rapidly-growing cells was completely reversed as a result of a block in sterol synthesis causing a negligible synthesis of delta 5,7-sterols. Two physiological parameters, budding frequency and membrane composition, evidently affected ethanol tolerance. Differences between the delta 5,7-sterol-synthesizing and deficient strains documented a profound effect of the quality of the sterol present on the physiological state of the cell.
authors.affiliation: Institute of Microbiology, Czechoslovak Academy of Sciences, Prague.
authors.forename: C
authors.initials: C
authors.lastname: Novotný
authors.forename: M
authors.initials: M
authors.lastname: Flieger
authors.forename: J
authors.initials: J
authors.lastname: Panos
authors.forename: L
authors.initials: L
authors.lastname: Dolezalová
mesh_terms: D000693:Anaerobiosis / Q000502:physiology; D000431:Ethanol / Q000494:pharmacology*; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000254:growth & development* / Q000378:metabolism; D013261:Sterols / Q000378:metabolism
chemical_list: D013261:Sterols; D000431:Ethanol