title: Tests of human sperm function and fertilization in vitro.
abstract: OBJECTIVE
  To review recent studies on the development of new tests of human sperm function and evaluation of which sperm characteristics are most important for fertilization in vitro by logistic regression analysis.
  
  
  METHODS
  Recent studies on the relationship between putative and new tests of human sperm function and fertility in vitro or in vivo are discussed in this review. Some physiological and technical aspects are included.
  
  
  METHODS
  Fertilization rates in vitro and sperm tests including standard semen analysis, improved morphology assessment, objective assessment of sperm motility and movement characteristics, nuclear maturity, hypo-osmotic swelling, the acrosome and the acrosome reaction, acrosin activity, human sperm-hamster oocyte penetration assay, and sperm-zona pellucida (ZP) and sperm-oolemma binding.
  
  
  RESULTS
  The percentages of sperm with normal morphology and a normal intact acrosome, mean linearity, and the number of sperm binding to the ZP were highly significant related to fertilization rates in vitro. Other sperm tests evaluated usually provided no additional information about fertilization rates. The human ZP is highly selective for binding of morphologically normal sperm. Acrosome-reacted human sperm have little or no ability to bind to the ZP.
  
  
  CONCLUSIONS
  Results of in vitro fertilization can be used to evaluate tests of human sperm function. Logistic regression analysis is a powerful method for determining which groups of sperm characteristics are independently related to fertilization rates. Normal morphology, linearity, acrosome status, and sperm-ZP binding are the most important characteristics for fertilization in vitro.
authors.affiliation: Department of Obstetrics and Gynaecology, University of Melbourne, Royal Women's Hospital, Victoria, Australia.
authors.forename: D Y
authors.initials: DY
authors.lastname: Liu
authors.forename: H W
authors.initials: HW
authors.lastname: Baker
mesh_terms: D000177:Acrosome / Q000502:physiology; D000818:Animals; D006224:Cricetinae; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D008297:Male; D013081:Sperm Motility; D013084:Sperm-Ovum Interactions; D013094:Spermatozoa / Q000502:physiology* / Q000648:ultrastructure