title: Interleukin-1 beta stimulates growth of adrenocortical cells in primary culture.
abstract: Using tritiated-thymidine incorporation as a measure of cell growth, interleukin-1 beta stimulated the growth of bovine zona fasciculata/reticularis adrenocortical cells after 72 h in primary culture. Within the range of 10-1000 pg/ml, interleukin-1 beta produced over 40% of angiotensin II-stimulated [3H]thymidine incorporation (P less than 0.005 compared with basal for 10 pg/ml and 1000 pg/ml; P less than 0.05 for 100 pg/ml; two-tailed unpaired Student's t-test). Interleukin-1 beta did not directly stimulate cortisol secretion. By stimulating adrenocortical growth, the increase in interleukin-1 during fever provides a potential mechanism for chronically raising glucocorticoid output. This study is the first demonstration of a long-term effect involving interleukin-1 beta on the adrenal cortex.
authors.affiliation: Department of Clinical Chemistry, Royal Infirmary, Edinburgh, U.K.
authors.forename: N
authors.initials: N
authors.lastname: Hanley
authors.forename: B C
authors.initials: BC
authors.lastname: Williams
authors.forename: M
authors.initials: M
authors.lastname: Nicol
authors.forename: I M
authors.initials: IM
authors.lastname: Bird
authors.forename: S W
authors.initials: SW
authors.lastname: Walker
mesh_terms: D000818:Animals; D002417:Cattle; D002455:Cell Division / Q000502:physiology; D002478:Cells, Cultured; D006801:Humans; D006854:Hydrocortisone / Q000378:metabolism; D007375:Interleukin-1 / Q000502:physiology*; D011994:Recombinant Proteins; D013936:Thymidine; D015383:Zona Fasciculata / Q000166:cytology*; D015385:Zona Reticularis / Q000166:cytology*
chemical_list: D007375:Interleukin-1; D011994:Recombinant Proteins; D013936:Thymidine; D006854:Hydrocortisone