Stability of retinoblastoma gene expression determines the tumorigenicity of reconstituted retinoblastoma cells.
Mutational inactivation of the retinoblastoma gene (RB) is an invariant feature of the childhood eye cancer retinoblastoma and of tumor cells derived therefrom. In a previous study, retrovirus-mediated transfer of wild-type RB into cultured retinoblastoma cells resulted in a marked enlargement and reduced growth rate of these cells, as well as loss of their tumorigenic properties in nude mice. It was therefore difficult to separate the proposed growth-suppressing and tumor-suppressing activities of RB protein. Here, we show that clones of RB-reconstituted retinoblastoma cells can be isolated that stably express apparently normal RB protein for at least 20 months of continuous culture. These clones were indistinguishable from nonreconstituted cells by multiple parameters including morphology, growth rate, and cell cycle distribution. Despite similar phenotypes in culture, clones with stable RB expression were uniformly nontumorigenic in nude mice, whereas those that lost such expression regained their tumorigenic properties. These results indicate that the tumorigenicity of these cells is entirely determined by the presence or absence of exogenous RB protein expression and that suppression of tumorigenicity is distinct from inhibition of cellular growth in culture.
Chen
P L
PL
Center for Molecular Medicine, University of Texas Health Science Center, San Antonio 78245.
Chen
Y
Y
Shan
B
B
Bookstein
R
R
Lee
W H
WH
D000818:Animals; D002453:Cell Cycle; D002455:Cell Division; D002999:Clone Cells; D005134:Eye Neoplasms / Q000235:genetics* / Q000473:pathology; D015870:Gene Expression; D051379:Mice; D008819:Mice, Nude; D012175:Retinoblastoma / Q000235:genetics* / Q000473:pathology; D014162:Transfection