Application of a microbial sensor for determination of short-chain fatty acids in raw milk samples.
A microbial sensor system, based on the use of immobilized Arthrobacter nicotiana and an oxygen electrode, was applied to determine free short-chain fatty acids in raw milk samples and the result was compared with gas chromatography (GC) and a titrimetric method. The sensor response was linearly related to the concentration of short-chain fatty acids obtained by GC (n = 10, r = 0.92) and to the total concentration of free fatty acids obtained by titrimetric measurement (n = 10, r = 0.78). This result suggests that the present microbial sensor can selectively determine free short-chain fatty acids in raw milk samples and may be useful as a very fast detection method of rancidity in milk.
Ukeda
H
H
GBF-Gesellschaft für Biotechnische Forschung, Department of Enzymtechnology, Braunschweig, Federal Republic of Germany.
Wagner
G
G
Weis
G
G
Miller
M
M
Klostermeyer
H
H
Schmid
R D
RD
D000818:Animals; D001681:Biological Assay; D002417:Cattle; D002849:Chromatography, Gas; D005230:Fatty Acids, Nonesterified / Q000032:analysis; D005232:Fatty Acids, Volatile / Q000032:analysis; D008892:Milk / Q000032:analysis*
D005230:Fatty Acids, Nonesterified; D005232:Fatty Acids, Volatile