Big game species identification by deoxyribonucleic acid (DNA) probes.
Species identification is important in many big game forensic science cases but cannot always be accomplished because of the lack of adequate techniques. The authors have developed deoxyribonucleic acid (DNA) probes for elk, deer, and antelope by isolating highly repeated satellite sequences. These DNA probes distinguish among deer, elk, and antelope, although not between different species of deer. Because of the high number of sequence copies per genome, these probes are extremely sensitive, requiring less than 10 ng of total genomic DNA. The developmental protocol for these probes is relatively simple and is applicable to many other species.
Blackett
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RS
Northern Regional Crime Laboratory, Arizona Department of Public Safety, Flagstaff.
Keim
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P
D000818:Animals; D000835:Animals, Wild / Q000145:classification / Q000235:genetics*; D000866:Antelopes / Q000145:classification / Q000235:genetics*; D015342:DNA Probes; D004276:DNA, Satellite / Q000032:analysis* / Q000737:chemistry; D003670:Deer / Q000145:classification / Q000235:genetics*; D004587:Electrophoresis, Agar Gel; D005554:Forensic Medicine / Q000379:methods; D009693:Nucleic Acid Hybridization; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D012680:Sensitivity and Specificity; D013045:Species Specificity
D015342:DNA Probes; D004276:DNA, Satellite