title: [Recent advances in EDRF research].
abstract: We have developed a new bioassay system for endothelium-derived relaxing factor (EDRF), using endothelial cells in culture. Bovine pulmonary arterial endothelial cells (BPAE) were grown on microcarrier beads and superfused in a cell column. The effluent was applied to bioassay tissues and biological and the chemical characters of EDRF were examined and compared with those of nitric oxide (NO). EDRF was vascular selective and negatively charged, whereas NO was a general relaxant and not charged. The hypothesis that EDRF is identical to NO is discussed. We also examined the interaction of some EDRF agonists with endothelial cells and biological characteristics of the agonists were discussed.
authors.affiliation: Division of Cardiovascular Surgery, Toyooka General Hospital.
authors.forename: K
authors.initials: K
authors.lastname: Shikano
mesh_terms: D000818:Animals; D001681:Biological Assay / Q000379:methods*; D000001:Calcimycin / Q000494:pharmacology; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000378:metabolism; D006801:Humans; D009569:Nitric Oxide / Q000032:analysis* / Q000378:metabolism / Q000502:physiology
chemical_list: D009569:Nitric Oxide; D000001:Calcimycin