title: Recovery in hemiparkinsonian rats following intrastriatal implantation of activated leukocytes.
abstract: Behavioral improvement has been seen in hemiparkinsonian animals after surgical lesions of the denervated caudate nucleus. This study was designed to investigate the role of inflammatory cells in injury-induced recovery. A hemiparkinsonian syndrome was induced in rats by unilateral injections of 6-hydroxydopamine into the pars compacta of the substantia nigra. Phytohemagglutinin-stimulated rat peritoneal cells, predominantly T cells and macrophages, were stereotactically implanted in the lesioned caudate-putamen, and amphetamine-induced turning was used to assess recovery. Animals receiving implants of activated peritoneal cells showed a 47% decrease in amphetamine-induced turning 8 weeks after implantation, which was not seen in control or sham-operated animals. Immunocytochemistry revealed increased tyrosine hydroxylase reactive fibers in the leukocyte-implanted striatum. We conclude that implantation of activated leukocytes promotes functional recovery in hemiparkinsonian rats.
authors.affiliation: Surgical Neurology Branch, National Institute of Neurological Diseases and Stroke, National Institutes of Health, Bethesda, MD 20892.
authors.forename: S E
authors.initials: SE
authors.lastname: Ewing
authors.forename: R J
authors.initials: RJ
authors.lastname: Weber
authors.forename: A
authors.initials: A
authors.lastname: Zauner
authors.forename: R J
authors.initials: RJ
authors.lastname: Plunkett
mesh_terms: D000818:Animals; D002421:Caudate Nucleus / Q000187:drug effects / Q000503:physiopathology*; D003342:Corpus Striatum / Q000187:drug effects / Q000503:physiopathology*; D003714:Denervation; D003913:Dextroamphetamine / Q000494:pharmacology; D005727:Ganglia, Spinal / Q000502:physiology; D037102:Lectins; D017708:Leukocyte Transfusion; D008213:Lymphocyte Activation; D008262:Macrophage Activation; D008264:Macrophages / Q000637:transplantation; D008297:Male; D016627:Oxidopamine / Q000633:toxicity; D010300:Parkinson Disease / Q000503:physiopathology* / Q000601:surgery; D051381:Rats; D011919:Rats, Inbred Strains; D013239:Stereotyped Behavior / Q000187:drug effects; D013378:Substantia Nigra / Q000473:pathology / Q000503:physiopathology*; D013601:T-Lymphocytes / Q000637:transplantation
chemical_list: D037102:Lectins; D016627:Oxidopamine; D003913:Dextroamphetamine