Effect of herpes simplex virus type 1 infection on cytokine gene expression in activated murine peritoneal macrophages.
The intrinsic resistance to herpes simplex virus type 1 (HSV-1) of murine peritoneal macrophages (PM phi) obtained after in vivo infection of different stimuli has been investigated and shown to vary depending on the state of M phi activation. Activation of M phi by C. parvum (CP-M phi) or by an avirulent strain of S. typhimurium (Sal-M phi) increased the permissiveness of M phi to HSV-1 infection as evidenced by increased HSV-1 immediate early (IE) gene expression, synthesis of IE proteins, and the degree of cytopathic effect. HSV-1 infection was also found to sharply reduce the level of IL-1-beta mRNA in CP-M phi) and Sal-M phi, and the level of IL-3 mRNA in infected Sal-M phi, as measured by northern blot hybridization. Barely detectable levels of IL-beta mRNA were found in Sal-M phi after infection with HSV-1 when the polymerase chain reaction (PCR) assay was used to confirm the reduction of IL-1-beta mRNA. These data suggest that HSV-1 infection can modulate gene expression of some cytokines in the activated M phi.
Wu
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Department of Microbiology and Immunology, Medical College of Pennsylvania, Philadelphia 19129.
Eisenstein
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Morahan
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D000818:Animals; D005260:Female; D015870:Gene Expression / Q000187:drug effects*; D006561:Herpes Simplex / Q000235:genetics* / Q000276:immunology; D007375:Interleukin-1 / Q000235:genetics; D007377:Interleukin-3 / Q000235:genetics; D007378:Interleukins / Q000235:genetics*; D008262:Macrophage Activation / Q000187:drug effects*; D008264:Macrophages / Q000187:drug effects*; D051379:Mice; D014709:Vero Cells
D007375:Interleukin-1; D007377:Interleukin-3; D007378:Interleukins