title: Dictyostelium giganteum plasmid Dgp1 is a member of the Ddp2 plasmid family.
abstract: Dgp1, a circular 4.4-kb plasmid found in the nuclei of Dictyostelium giganteum strain DG61, is a member of the same plasmid family as plasmids Ddp2 and pDG1. Dgp1 has sequence similarity to a conserved region of the Ddp2 and pDG1 open reading frames. As with Ddp2 and pDG1, a single large RNA is transcribed from Dgp1. This 3.3-kb transcript is present at about 350 copies per vegetative cell. The transcript abundance decreased about 10-fold in early aggregation and continued at this lower level until late culmination when it returned to the level seen in vegetative cells. Dgp1 has a repeat of several hundred base pairs in a location, relative to the transcribed region, similar to the inverted repeats found in Ddp2 and pDG1. Dgp1 cannot be maintained as a plasmid in Dictyostelium discoideum AX4 cells, suggesting that Dgp1 carries species-specific maintenance elements.
authors.affiliation: Department of Biology, Utah State University, Logan 84322-5500.
authors.forename: Y
authors.initials: Y
authors.lastname: Yin
authors.forename: D L
authors.initials: DL
authors.lastname: Welker
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002467:Cell Nucleus / Q000648:ultrastructure; D003001:Cloning, Molecular; D004270:DNA, Circular / Q000235:genetics; D004023:Dictyostelium / Q000235:genetics*; D014644:Genetic Variation; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic
chemical_list: D004270:DNA, Circular