title: CEDIA--homogeneous immunoassays for the 1990s and beyond.
abstract: New homogeneous enzyme immunoassays have been developed for cortisol, digoxin, digitoxin, theophylline, phenytoin, and phenobarbital using the cloned enzyme donor immunoassay technology. As applied to Boehringer Mannheim/Hitachi analysis systems these methods provide rapid, accurate and precise quantification of analytes, with minimal interferences from endogenous serum constituents and low cross-reactivities to structurally-related hormonal precursors, drug metabolites and natural compounds. Additional significant features of the new assays are linear standard curves and two-point calibration. The six CEDIA assays join the two currently available CEDIA assays for determination of the thyroid parameters T4 and T Uptake. Additional new therapeutic drug and anemia monitoring assays are under development, demonstrating the versatility of the cloned enzyme donor immunoassay technology. These tests, in concert with Boehringer Mannheim/Hitachi analyzers, provide a high throughput, random access immunoassay system. The menu of available assays should continue to increase during the 1990s, providing efficient automation while allowing consolidation of testing on a limited number of instrument systems.
authors.affiliation: Microgenics Corporation, Conford, California.
authors.forename: W A
authors.initials: WA
authors.lastname: Coty
authors.forename: R
authors.initials: R
authors.lastname: Loor
authors.forename: N
authors.initials: N
authors.lastname: Bellet
authors.forename: P L
authors.initials: PL
authors.lastname: Khanna
authors.forename: P
authors.initials: P
authors.lastname: Kaspar
authors.forename: M
authors.initials: M
authors.lastname: Baier
mesh_terms: D004074:Digitoxin / Q000493:pharmacokinetics; D004077:Digoxin / Q000493:pharmacokinetics; D004305:Dose-Response Relationship, Drug; D016903:Drug Monitoring / Q000379:methods*; D006801:Humans; D006854:Hydrocortisone / Q000097:blood; D007124:Immunoenzyme Techniques; D010634:Phenobarbital / Q000493:pharmacokinetics; D010672:Phenytoin / Q000493:pharmacokinetics; D013806:Theophylline / Q000493:pharmacokinetics
chemical_list: D010672:Phenytoin; D004077:Digoxin; D013806:Theophylline; D004074:Digitoxin; D006854:Hydrocortisone; D010634:Phenobarbital