Near-infrared FT-Raman spectra of the rat brain tissues.
Near-infrared Fourier transform (FT) Raman spectroscopy was applied to brain tissues in situ. The spectra were obtained from the cerebral cortex, white matter of the cerebrum, caudate-putamen, thalamus, synaptosomal fraction, and myelin fraction. High-quality Raman spectra in the 400 to 2940 cm-1 range were measured without interference of autofluorescence. Common spectral bands were assigned. The ratios of the intensity at 1664 (amide I), 1442 (CH2 deformation), 2885 (CH2 asymmetric stretching), 2938 cm-1 (CH3 symmetric stretching) could be used for differentiation between the gray and white matters.
Mizuno
A
A
Department of Biochemistry, Jikei University School of Medicine, Tokyo, Japan.
Hayashi
T
T
Tashibu
K
K
Maraishi
S
S
Kawauchi
K
K
Ozaki
Y
Y
D000818:Animals; D001923:Brain Chemistry; D005227:Fatty Acids / Q000032:analysis; D005583:Fourier Analysis; D008297:Male; D009186:Myelin Sheath / Q000737:chemistry; D009419:Nerve Tissue Proteins / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013059:Spectrum Analysis, Raman; D013574:Synaptosomes / Q000737:chemistry
D005227:Fatty Acids; D009419:Nerve Tissue Proteins