Characterization of bacteriophage BFK20 from Brevibacterium flavum.
Bacteriophage BFK20 was isolated from a Brevibacterium flavum strain that had become contaminated during industrial fermentation. BFK20 has a polyhedral head 50 nm wide and a non-contractile tail 200 nm long and 10 nm in diameter. The genome of this bacteriophage consists of a linear double stranded DNA molecule of 44-45 kb with cohesive ends. The capsid of phage BFK20 contains nine polypeptides with molecular masses from 22.0-108.0 kDa. BFK20 DNA was used as a donor for fragments carrying promoters and transcription-terminators.
Koptides
M
M
Institute of Molecular Biology, Slovak Academy of Sciences, Bratislava, Czech.
Barák
I
I
Sisová
M
M
Baloghová
E
E
Ugorcaková
J
J
Timko
J
J
D001435:Bacteriophages / Q000235:genetics* / Q000378:metabolism / Q000648:ultrastructure; D001951:Brevibacterium; D004591:Electrophoresis, Polyacrylamide Gel; D016679:Genome, Viral; D008854:Microscopy, Electron; D011401:Promoter Regions, Genetic; D015183:Restriction Mapping; D014158:Transcription, Genetic