title: The role of saliva in aggregation and adherence of Pseudomonas aeruginosa in patients with cystic fibrosis.
abstract: The aggregation and adherence activity of P. aeruginosa, mediated by whole saliva from cystic fibrosis (CF) patients and non-CF subjects, was investigated. CF saliva-mediated aggregation of P. aeruginosa was stronger than the activity of non-CF saliva. Likewise, P. aeruginosa adherence to buccal epithelial cells (BEC) of CF patients was stronger than to BEC of non-CF subjects. Adherence of non-mucoid P. aeruginosa to BEC of CF patients was increased by saliva, whereas the mucoid variant was not. CF patients colonized with P. aeruginosa showed higher adherence of the non-mucoid variant than non-colonized CF patients. CF patients with high saliva-mediated adherence of non-mucoid P. aeruginosa also had high salivary aggregation activity. Increased CF saliva-mediated aggregation activity may be linked to the increased non-mucoid P. aeruginosa adherence to BEC of CF patients.
authors.affiliation: Department of Oral Biology, Royal University Hospital, University of Saskatchewan, Saskatoon, Canada.
authors.forename: S K
authors.initials: SK
authors.lastname: Tumber-Saini
authors.forename: B F
authors.initials: BF
authors.lastname: Habbick
authors.forename: A M
authors.initials: AM
authors.lastname: Oles
authors.forename: J P
authors.initials: JP
authors.lastname: Schaefer
authors.forename: K
authors.initials: K
authors.lastname: Komiyama
mesh_terms: D000293:Adolescent; D000704:Analysis of Variance; D001422:Bacterial Adhesion / Q000502:physiology*; D002648:Child; D003550:Cystic Fibrosis / Q000382:microbiology* / Q000503:physiopathology*; D003584:Cytological Techniques; D004198:Disease Susceptibility; D005260:Female; D006801:Humans; D008297:Male; D008828:Microbiological Techniques; D011550:Pseudomonas aeruginosa / Q000145:classification / Q000502:physiology*; D012463:Saliva / Q000502:physiology*