title: Sensitive determination of josamycin and rokitamycin in plasma by high-performance liquid chromatography with fluorescence detection.
abstract: Rokitamycin and josamycin were successfully derivatized with dansylhydrazine in 20 min at 60 degrees C. Rokitamycin and josamycin levels were determined in plasma after ion-pair extraction into hexane-isoamyl alcohol with lauryl sulphate and precolumn derivatization. Resolution was obtained by liquid chromatography with fluorescence detection (352/537 nm) in 12 min. The limit of detection was 20 ng/ml macrolide starting from 1 ml of plasma, and linearity was demonstrated between 50 and 400 ng/ml. Inter-run coefficients of variation were 10.2% at 100 ng/ml and 9.1% at 300 ng/ml. The system was reliably used for pharmacokinetic studies in plasma.
authors.affiliation: Department of Pharmacotoxicology, Avicenne Hospital, Bobigny, France.
authors.forename: M
authors.initials: M
authors.lastname: Tod
authors.forename: O
authors.initials: O
authors.lastname: Biarez
authors.forename: P
authors.initials: P
authors.lastname: Nicolas
authors.forename: O
authors.initials: O
authors.lastname: Petitjean
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005453:Fluorescence; D006801:Humans; D015570:Josamycin / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D015644:Miocamycin / Q000031:analogs & derivatives* / Q000097:blood / Q000737:chemistry / Q000493:pharmacokinetics
chemical_list: D015644:Miocamycin; D015570:Josamycin; C033383:rokitamycin