title: Interaction of ubiquinone and vitamin K3 with mitochondrial succinate-ubiquinone oxidoreductase.
abstract: Two new methods have been devised for measuring fumarate reduction by beef heart succinate-ubiquinone oxidoreductase with quinols as electron donors. In one assay the quinone is maintained in the reduced state by coupling fumarate reduction with the DT-diaphorase reaction, in the other assay by the presence of excess dithionite. The advantages of these methods are discussed, along with preliminary characterization of the quinol-protein interaction.
authors.affiliation: Dept. of Biochemistry, Tel Aviv University, Ramat Aviv, Israel.
authors.forename: A B
authors.initials: AB
authors.lastname: Kotlyar
authors.forename: M
authors.initials: M
authors.lastname: Gutman
authors.forename: B A
authors.initials: BA
authors.lastname: Ackrell
mesh_terms: D000818:Animals; D002417:Cattle; D004227:Dithionite / Q000494:pharmacology; D042963:Electron Transport Complex II; D007700:Kinetics; D008929:Mitochondria, Heart / Q000201:enzymology*; D009097:Multienzyme Complexes / Q000378:metabolism*; D016660:NAD(P)H Dehydrogenase (Quinone) / Q000378:metabolism; D010088:Oxidoreductases / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet; D013385:Succinate Dehydrogenase / Q000378:metabolism*; D014451:Ubiquinone / Q000378:metabolism*; D014812:Vitamin K / Q000378:metabolism*
chemical_list: D009097:Multienzyme Complexes; D014812:Vitamin K; D014451:Ubiquinone; D004227:Dithionite; D010088:Oxidoreductases; D042963:Electron Transport Complex II; D013385:Succinate Dehydrogenase; D016660:NAD(P)H Dehydrogenase (Quinone)