Simultaneous determination of a new gastrointestinal prokinetic agent (HSR-803) and its metabolites in human serum and urine by high-performance liquid chromatography using automated column-switching.
A method based on high-performance liquid chromatography using column-switching is described for the simultaneous determination of HSR-803 and its metabolites in human serum and urine. The system uses a six-port valve with a Nucleosil CN pre-column for on-line sample clean-up, and direct injection of samples. The limits of quantitation in serum and urine were 5 and 20 ng/ml for HSR-803 and 50 and 200 ng/ml for the metabolites, respectively. The coefficients of variation for the intra- and inter-day accuracies were between 0.8 and 7.1% for each compound. This method was applied to the pharmacokinetic studies in humans after oral administration of HSR-803.
Takahara
E
E
Central Research Laboratories, Hokuriku Seiyaku Co., Ltd., Fukui, Japan.
Fukuoka
H
H
Takagi
T
T
Nagata
O
O
Kato
H
H
D001549:Benzamides / Q000032:analysis* / Q000493:pharmacokinetics; D001593:Benzyl Compounds / Q000032:analysis* / Q000493:pharmacokinetics; D002800:Cholinesterase Inhibitors / Q000032:analysis* / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid; D018492:Dopamine Antagonists; D006801:Humans; D007202:Indicators and Reagents; D008297:Male
D001549:Benzamides; D001593:Benzyl Compounds; D002800:Cholinesterase Inhibitors; D018492:Dopamine Antagonists; D007202:Indicators and Reagents; C064878:N-(4-(2-(dimethylamino)ethoxy)benzyl)-3,4-dimethoxybenzmide