The differentiation of Chlamydia species by antigen detection in sputum specimens from patients with community-acquired acute respiratory infections.
An amplified enzyme immunoassay (IDEIA III: Dako Diagnostics Ltd) for detecting genus-specific chlamydia antigen was evaluated prospectively on 286 respiratory specimens from 275 patients presenting with community-acquired pneumonia or persistent chest infection. Nineteen patients had evidence of recent chlamydial infection, having two or more positive sputum or serological markers. Sputa from two other patients were ELISA-positive in the absence of other positive criteria and were regarded as false-positive results. When compared with a direct immunofluorescence test for chlamydial elementary bodies (EBs) using a genus-specific monoclonal antibody, the ELISA gave a positive predictive value of 91% and a negative predictive value of 99%. Non-specific problems with a wide variety of other micro-organisms isolated from the sputa were not encountered. Attempts to differentiate between Chlamydia psittaci, Chlamydia pneumoniae and Chlamydia trachomatis using genus-specific lipopolysaccharide reactive--and species-specific major outer membrane protein--monoclonal antibodies were encouraging and results were substantiated, in most patients, by the species-specific serological assays of the whole-cell-inclusion immunofluorescence or micro-immunofluorescence assays. The study demonstrated that antigen detection techniques offer scope for routine laboratories to diagnose chlamydial respiratory infections rapidly and reliably and may enable differentiation to species level. Although immunofluorescence offers marginally greater sensitivity and specificity when compared with ELISA, the latter is less subjective and less demanding. Sixty-eight per cent of these infections would have remained undiagnosed despite the general availability of ELISA tests.
Sillis
M
M
PHLS/Microbiology Department, West Norwich Hospital, UK.
White
P
P
Caul
E O
EO
Paul
I D
ID
Treharne
J D
JD
D000328:Adult; D000907:Antibodies, Bacterial / Q000032:analysis; D000942:Antigens, Bacterial / Q000302:isolation & purification*; D001431:Bacteriological Techniques; D002689:Chlamydia / Q000145:classification* / Q000302:isolation & purification; D002690:Chlamydia Infections / Q000175:diagnosis / Q000382:microbiology*; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D006801:Humans; D008875:Middle Aged; D012141:Respiratory Tract Infections / Q000175:diagnosis / Q000382:microbiology*; D013183:Sputum / Q000382:microbiology*
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial