Monoclonal antibodies against metallothioneins from the human liver.
Six hybridomas secreting monoclonal antibodies directed against human fetal liver metallothioneins (MTs) have been generated and characterized. One antibody, K5A6, was specific for MT-1, the others recognized all isoMTs present in the human fetal liver. Each of these antibodies showed a unique cross-reactivity pattern when tested with MTs from the livers of different mammals. A double-antibody sandwich enzyme-linked-immunosorbent-assay (ELISA) was developed with the antibodies L2E3 and L5G2. This assay allows the detection of 60 pg human fetal liver MT and exhibits a metal dependent response for Zn-, Cd- and Hg-MT.
Van Houdt
K
K
Katholieke Universiteit Leuven, Laboratorium voor Biochemie, Belgium.
Nicasi
I
I
Van Mechelen
E
E
Veulemans
B
B
De Ley
M
M
D000328:Adult; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D003429:Cross Reactions; D004285:Dogs / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007668:Kidney / Q000737:chemistry; D008099:Liver / Q000737:chemistry* / Q000196:embryology; D008668:Metallothionein / Q000145:classification / Q000276:immunology* / Q000302:isolation & purification; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology; D010215:Papio / Q000276:immunology; D011817:Rabbits / Q000276:immunology; D051381:Rats / Q000276:immunology
D000911:Antibodies, Monoclonal; D008668:Metallothionein