Expression of protein kinase C subspecies in rat retina.
An extract of rat retina was subjected to Mono Q followed by chromatography on hydroxyapatite, and the protein kinase C (PKC) subspecies were identified by immunoblot and biochemical analysis. It was found that, although the relative activities assayed with myelin basic protein as a common phosphate acceptor vary greatly with one another, the alpha-, beta I-, beta II-, gamma-, delta-, epsilon-, zeta-, and another structurally unknown PKC subspecies are expressed in this tissue. Thus, the retina is a unique tissue which expresses most of the PKC subspecies so far identified in mammals.
Fujisawa
N
N
Department of Biochemistry, Kobe University School of Medicine, Japan.
Ogita
K
K
Saito
N
N
Nishizuka
Y
Y
D000818:Animals; D000918:Antibody Specificity; D015151:Immunoblotting; D007700:Kinetics; D008297:Male; D011493:Protein Kinase C / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012160:Retina / Q000201:enzymology*
D011493:Protein Kinase C