Internal-surface reversed-phase chromatography for plasma metabolite analysis of radiopharmaceuticals.
The use of internal-surface reversed-phase (ISRP) chromatography of unprocessed plasma samples was investigated as an alternative method of quantitation of the arterial plasma metabolite time course of [18F]N-methylspiperone. The ISRP method was directly compared to standard solid phase extraction/HPLC (SPE/HPLC) methods currently in wide use. Results indicate that: (1) the ISRP method is rapid and minimizes sample preparation; (2) recovery of radioactivity from the ISRP column is greater than 90%; (3) no radioactivity remains associated with chromatographically size excluded proteins and (4) the quantitative results are well correlated with conventional SPE/HPLC methods.
Ehrenkaufer
R L
RL
Cerebrovascular Research Center, University of Pennsylvania, Philadelphia 19104-6063.
Klam
S
S
Makoroff
K
K
Giandinoto
S
S
Morton
T
T
Moroney
D
D
Nowak
P
P
D000818:Animals; D001798:Blood Proteins / Q000378:metabolism; D002498:Centrifugation / Q000379:methods; D002845:Chromatography / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005069:Evaluation Studies as Topic; D005462:Fluorine Radioisotopes / Q000097:blood*; D006801:Humans; D010215:Papio; D011485:Protein Binding; D051381:Rats; D011919:Rats, Inbred Strains; D013134:Spiperone / Q000031:analogs & derivatives* / Q000097:blood; D014055:Tomography, Emission-Computed
D001798:Blood Proteins; D005462:Fluorine Radioisotopes; D013134:Spiperone; C039167:3-N-methylspiperone