title: Enhancement of the antibody response to protein antigens by specific IgG under different experimental conditions.
abstract: Specific stimulation of the antibody response to protein antigens by IgG antibodies was studied in vivo. The response to TNP-coupled keyhole limpet haemocyanin (KLH-TNP) was enhanced by a TNP-specific IgG monoclonal antibody, 7B4, as measured both in ELISA (enzyme-linked immunosorbent assay) and by ELISPOT (enzyme-linked immunospot) method. Enhancement was seen when using both high and low doses of antigen and antibody. The antibody response to TNP-coupled bovine serum albumin (BSA-TNP), but not to TNP-coupled ovalbumin (OA-TNP), tetanus toxoid (TT-TNP) or diphtheria toxoid (DT-TNP), was also efficiently augmented by 7B4. Enhancement was seen when using both high and low coupling ratio of TNP to KLH. The fact that IgG-mediated enhancement is seen under many different experimental conditions suggests that it is a physiologically relevant phenomenon. In order to enhance the response to KLH-TNP and BSA-TNP, 7B4 had to be injected while antigen was circulating in blood. This supports that IgG-mediated stimulation acts by concentrating circulating antigen into lymphoid centres.
authors.affiliation: Department of Immunology, BMC, Uppsala, Sweden.
authors.forename: E J
authors.initials: EJ
authors.lastname: Wiersma
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000917:Antibody Formation; D000941:Antigens / Q000276:immunology*; D006433:Hemocyanins / Q000276:immunology; D007074:Immunoglobulin G / Q000502:physiology*; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D014301:Trinitrobenzenes / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D000941:Antigens; D007074:Immunoglobulin G; D014301:Trinitrobenzenes; D006433:Hemocyanins; C032808:keyhole-limpet hemocyanin