Identification and purification of overlapping cosmid clones of the region Xq24-qter of human X chromosome using HPLC.
The assembly of a large physical map of genomes requires simultaneous analysis of many cosmid clones for overlapping regions. The search for overlapping regions may be achieved by various means. High-performance liquid chromatography (HPLC) provides an alternative to gel electrophoresis since microgram amounts of each DNA fragment may be collected into individual test tubes for further analysis. HPLC has been used to identify overlapping cosmid clones from a pool of cosmid DNA containing the terminal portion of the long arm of the human X chromosome (Xq24-qter). Among 400 cosmids analyzed, 3 were shown to overlap.
Ida
B
B
Istituto Tecnologie Biomediche Avanzate, Milan, Italy.
Pierluigi
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M
Lucia
S
S
Piergiorgio
P
P
D002851:Chromatography, High Pressure Liquid; D003001:Cloning, Molecular; D003360:Cosmids; D004587:Electrophoresis, Agar Gel; D006801:Humans; D009693:Nucleic Acid Hybridization; D015347:RNA Probes; D014960:X Chromosome
D015347:RNA Probes