Localisation of cis elements in the promoter of a wheat alpha-Amy2 gene.
A functional analysis of the promoter from the wheat alpha-amylase gene alpha-Amy2/54 is described. Mutant alpha-Amy2/54 promoters containing replacements or deletions were constructed and their ability to direct expression of the reporter gene beta-glucuronidase (GUS) in gibberellin-responsive oat aleurone protoplasts analysed. Chimaeric promoters using regions of the cauliflower mosaic virus (CaMV) 35S and alpha-Amy2/54 promoters were also analysed. The results suggest that at least three regions within the alpha-Amy2/54 promoter contain cis elements that are necessary for high-level gibberellin-regulated transcription. Fusion of 1.8 kb of promoter sequence upstream from -117 bp to a minimal (-55 CaMV 35S) promoter gave rise to hormone-independent expression implying that the region 3' to -117 bp contains an element which represses transcription in the absence of gibberellin or presence of abscisic acid.
Huttly
A K
AK
Dept of Agricultural Sciences, University of Bristol, UK.
Phillips
A L
AL
Tregear
J W
JW
D001483:Base Sequence; D002678:Chimera / Q000235:genetics; D003001:Cloning, Molecular; D004247:DNA; D004742:Enhancer Elements, Genetic; D005786:Gene Expression Regulation; D008969:Molecular Sequence Data; D009029:Mosaic Viruses / Q000235:genetics; D016296:Mutagenesis; D011401:Promoter Regions, Genetic; D014908:Triticum / Q000201:enzymology / Q000235:genetics*; D000516:alpha-Amylases / Q000235:genetics* / Q000378:metabolism
D004247:DNA; D000516:alpha-Amylases