[Usefulness of monoclonal antibodies for immunophenotyping in leukemia].
Clinically useful monoclonal antibodies, applied for immunophenotyping of leukemias, are reviewed. With a combination of 15 antibodies, including CD2, CD3, CD4, CD5, CD7, and CD8 for T cell marker analysis, CD10, CD19, CD20, surface immunoglobulins, and cytoplasmic mu chain for B cell marker analysis, CD13 and CD33 for myeloid marker analysis, and HLA-DR and CD25 for other marker analysis, acute lymphoblastic leukemias of T cell type, cALL type, pre-B cell type and B cell type, acute myeloid leukemias, acute unclassified leukemias and adult T cell leukemias could be clearly diagnosed by immunophenotyping of cell membrane molecules. By using additional CD11b, CD14, and CD15 monoclonal antibodies, subclassification of acute myeloid leukemia was partially possible.
Sagawa
K
K
Department of Immunology, Kurume University School of Medicine.
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D006801:Humans; D016130:Immunophenotyping; D007938:Leukemia / Q000145:classification / Q000175:diagnosis*
D000911:Antibodies, Monoclonal; D015703:Antigens, CD