Molecular cloning of two partial serotonin 5-HT1D receptor sequences in mouse and one in guinea pig.
The G protein coupled serotonin (5-HT) receptors, with seven membrane spanning domains, form a multigene family of which several members have been cloned and sequenced. The presence of 5-HT1D binding sites to our knowledge has not yet been reported in mouse. Here we describe the cloning and sequencing by the polymerase chain reaction (PCR) method of two 5-HT1D receptor sequences of the third cytoplasmic loop in mouse, strongly suggesting the existence of two 5-HT1D receptor genes, located on chromosome 4. A homologous sequence to one of them was cloned in guinea pig.
Weydert
A
A
Unité de Pharmacologie neuro-immuno-endocrinienne, Institut Pasteur, Paris.
Cloez-Tayarani
I
I
Fillion
M P
MP
Simon-Chazottes
D
D
Guenet
J L
JL
Fillion
G
G
D000818:Animals; D001483:Base Sequence / Q000235:genetics; D015139:Blotting, Southern; D003001:Cloning, Molecular; D006168:Guinea Pigs; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011985:Receptors, Serotonin / Q000235:genetics*
D011985:Receptors, Serotonin