Limited cell-cycle dependence of the merocyanine 540-sensitized photoinactivation of L1210 leukemia cells.
L1210 leukemia cells were synchronized by a double thymidine block technique and then characterized with regard to their susceptibility to merocyanine 540 (MC540)-sensitized photoinactivation. Cells harvested 5 (G2/M phase) h after release from the second thymidine block were most susceptible to MC540-sensitized photoinactivation followed, in order of decreasing sensitivity, by cells harvested 2 (S phase) h and by cells harvested 7 (G1 phase) h after release from the second block. The expression of dye-binding sites changed very little during the cell cycle.
Qiu
K
K
Department of Pediatrics, Medical College of Wisconsin, Milwaukee 53226.
Traul
D L
DL
Sieber
F
F
D000818:Animals; D002453:Cell Cycle / Q000187:drug effects; D007939:Leukemia L1210 / Q000188:drug therapy*; D010778:Photochemotherapy; D011744:Pyrimidinones / Q000494:pharmacology*; D011838:Radiation-Sensitizing Agents / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000187:drug effects
D011744:Pyrimidinones; D011838:Radiation-Sensitizing Agents; C003954:merocyanine dye