title: Analysis of saxitoxin in urine by continuous-flow fast-atom bombardment mass spectrometry.
abstract: An improved method of saxitoxin analysis in urine using continuous-flow fast-atom bombardment mass spectrometry was developed. Parameters studied were matrix composition, matrix flow, temperature of probe tip, probe-tip design and sample extraction. Optimal detection was obtained using the following matrix composition: 5% glycerol, 0.5% acetic acid, 0.025% sodium dodecylsulfate, 0.1% polyethylene glycol (PEG) 400 and 0.5% PEG 300; probe-tip temperature: (approximately 55 degrees C); flow rate: 5 or 8 microL per min.; probe tip: Olson-Hogge design. The STX standard was detected at 200 pg with signal-to-noise ratio of 11. The percent recovery of saxitoxin from human urine after clean-up on a weak cation exchange column was 75%.
authors.affiliation: Department of Plant Pathology, University of Minnesota, St Paul 55108.
authors.forename: C J
authors.initials: CJ
authors.lastname: Mirocha
authors.forename: W
authors.initials: W
authors.lastname: Cheong
authors.forename: U
authors.initials: U
authors.lastname: Mirza
authors.forename: Y B
authors.initials: YB
authors.lastname: Kim
mesh_terms: D006801:Humans; D066298:In Vitro Techniques; D012530:Saxitoxin / Q000652:urine*; D016339:Spectrometry, Mass, Fast Atom Bombardment
chemical_list: D012530:Saxitoxin