Recovery in hemiparkinsonian rats following intrastriatal implantation of activated leukocytes.
Behavioral improvement has been seen in hemiparkinsonian animals after surgical lesions of the denervated caudate nucleus. This study was designed to investigate the role of inflammatory cells in injury-induced recovery. A hemiparkinsonian syndrome was induced in rats by unilateral injections of 6-hydroxydopamine into the pars compacta of the substantia nigra. Phytohemagglutinin-stimulated rat peritoneal cells, predominantly T cells and macrophages, were stereotactically implanted in the lesioned caudate-putamen, and amphetamine-induced turning was used to assess recovery. Animals receiving implants of activated peritoneal cells showed a 47% decrease in amphetamine-induced turning 8 weeks after implantation, which was not seen in control or sham-operated animals. Immunocytochemistry revealed increased tyrosine hydroxylase reactive fibers in the leukocyte-implanted striatum. We conclude that implantation of activated leukocytes promotes functional recovery in hemiparkinsonian rats.
Ewing
S E
SE
Surgical Neurology Branch, National Institute of Neurological Diseases and Stroke, National Institutes of Health, Bethesda, MD 20892.
Weber
R J
RJ
Zauner
A
A
Plunkett
R J
RJ
D000818:Animals; D002421:Caudate Nucleus / Q000187:drug effects / Q000503:physiopathology*; D003342:Corpus Striatum / Q000187:drug effects / Q000503:physiopathology*; D003714:Denervation; D003913:Dextroamphetamine / Q000494:pharmacology; D005727:Ganglia, Spinal / Q000502:physiology; D037102:Lectins; D017708:Leukocyte Transfusion; D008213:Lymphocyte Activation; D008262:Macrophage Activation; D008264:Macrophages / Q000637:transplantation; D008297:Male; D016627:Oxidopamine / Q000633:toxicity; D010300:Parkinson Disease / Q000503:physiopathology* / Q000601:surgery; D051381:Rats; D011919:Rats, Inbred Strains; D013239:Stereotyped Behavior / Q000187:drug effects; D013378:Substantia Nigra / Q000473:pathology / Q000503:physiopathology*; D013601:T-Lymphocytes / Q000637:transplantation
D037102:Lectins; D016627:Oxidopamine; D003913:Dextroamphetamine