Tyrosylprotein sulfotransferase in rat submandibular salivary glands.
1. The transfer of sulfate ester group from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to poly-(Glu6, Ala3, Tyr1) (EAY; Mr 47 kDa) in rat submandibular salivary gland has been investigated. The highest tyrosylprotein sulfotransferase activity was obtained in the Golgi-enriched fraction in the presence of 2 mM 5'AMP, 20 mM MnCl2 and 50 mM NaF at pH 6.2. 2. The apparent Km values for EAY and PAPS were 1.6 x 10(-6) and 1.9 x 10(-6) M, respectively. 3. Inclusion of NaCl, EDTA, NEM and DTT was inhibitory for the enzyme activity. The enzyme was 28 times less susceptible to 2,6-dichloro-4-nitrophenol inhibition than to phenol sulfotransferase inhibition. 4. This study is the first report characterizing a sulfotransferase activity specific for tyrosylprotein in rat submandibular salivary glands.
Sundaram
P
P
Research Center, University of Medicine and Dentistry of New Jersey, Newark 07103-2400.
Slomiany
A
A
Slomiany
B L
BL
Kasinathan
C
C
D000818:Animals; D003902:Detergents; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013363:Submandibular Gland / Q000201:enzymology*; D015238:Sulfotransferases / Q000378:metabolism*
D003902:Detergents; D015238:Sulfotransferases; C042860:glycoprotein sulfotransferase; C039079:protein-tyrosine sulfotransferase