Quinone dependent NADH dehydrogenation in mitochondria-like particles from Setaria digitata, a filarial parasite.
In the cattle filarial parasite, Setaria digitata, the mitochondria-like particles have been shown to possess site I associated oxidative phosphorylation and rotenone sensitive and insensitive pathways for the dehydrogenation of NADH. Quinone depleted mitochondria-like particles show a loss of activity of these NADH dehydrogenases and also a complete loss of fumarate reductase activity. Reconstitution with quinone restores both NADH linked oxygen uptake and fumarate reductase activity. Thus activities of complex I and fumarate reductase are linked to quinone. Hence an inhibitor at the level of quinone can simultaneously block both aerobic and anaerobic pathways which drive ATP production and may prove useful in the effective control of filariasis.
Sivan
V M
VM
Department of Biochemistry, University of Kerala Kariavattom, Thiruvananthapuram, India.
Raj
R K
RK
D000818:Animals; D000968:Antimycin A / Q000494:pharmacology; D002417:Cattle; D005370:Filarioidea / Q000302:isolation & purification / Q000378:metabolism*; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism*; D008954:Models, Biological; D009243:NAD / Q000378:metabolism*; D016660:NAD(P)H Dehydrogenase (Quinone) / Q000378:metabolism*; D009245:NADH Dehydrogenase / Q000378:metabolism*; D010084:Oxidation-Reduction; D010101:Oxygen Consumption / Q000187:drug effects; D010529:Peritoneal Cavity / Q000469:parasitology; D011809:Quinones / Q000378:metabolism*; D012402:Rotenone / Q000494:pharmacology; D014451:Ubiquinone / Q000378:metabolism
D011809:Quinones; D012402:Rotenone; D009243:NAD; D014451:Ubiquinone; D000968:Antimycin A; D016660:NAD(P)H Dehydrogenase (Quinone); D009245:NADH Dehydrogenase