The immobilization of all spermatozoa in vitro by bitter lemon drink and the effect of alkaline pH.
This study investigated the effects of increase in temperature and in pH of Coca-Cola, Afri-Cola, Pepsi-Cola and Krest bitter lemon drinks ("soft drinks") produced in Nigeria on the in vitro motility of spermatozoa. Semen was collected from 7 men, average age 28 years, of proven fertility, after 5 days' abstinence from sexual intercourse. The temperature and pH of the drinks were adjusted from 22 degrees C (room) to 37 degrees C, and pH 2.4 (acid) to 7.5 (alkaline), respectively. The mean % motility of spermatozoa in the adjusted and in the unadjusted drinks was compared for significant differences at the 1% level using the student's t-test. The results showed no significant differences in mean % motility in the drinks at 22 degrees C and at 37 degrees C. The mean % motility in all the drinks, except Coca-Cola, was significantly greater at alkaline than at acid pH; for Coca-Cola, motility was significantly greater at acid than alkaline pH. Of the drinks, Krest bitter lemon (unadjusted) immobilized all spermatozoa within 1 minute of addition.


CONCLUSIONS
i) alkalinity decreases the spermicidal action of all drinks except Coca-Cola, and ii) Krest bitter lemon may achieve very high efficacy if used as post-coital douche, especially in the impoverished, densely populated Third World.
Nwoha
P U
PU
Department of Anatomy and Cell Biology, College of Medical Sciences, Obafemi Awolowo University, Ile-Ife, Nigeria.
D000328:Adult; D001628:Beverages / Q000009:adverse effects; D002957:Citrus; D006801:Humans; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D008297:Male; D013081:Sperm Motility / Q000187:drug effects*; D013696:Temperature