Arachidonic acid metabolites: effects on inflammation of fetal rabbit excisional wounds.
Uncovered fetal rabbit excisional wounds do not exhibit any classic signs of healing; wounds covered with an impermeable cover do contract, reepithelialize, and exhibit inflammation. Prostaglandin E2 (PGE2) is elevated in amniotic fluid, acting as an immunosuppressant at the maternal-fetal interface. Full-thickness excisional wounds were made on 25-day gestational age rabbit fetuses. Half the wounds were covered with an impermeable cover. Tissue from covered, uncovered, and nonwounded fetuses was examined 72 h after wounding for arachidonic acid metabolites. Uncovered wounds had significantly (P less than or equal to 0.05) elevated levels of PGE2, PGF2 alpha, and 12-HETE versus covered wounds and control tissue. Covered wounds had significantly elevated levels of 15-HETE compared to uncovered and control tissue. The elevated PGE2 in uncovered wounds may act as a fetal immunosuppressant; covered wounds (lower PGE2) developed cellular inflammation. Further investigations of these interactions may permit modulation of adult inflammation.
Morykwas
M J
MJ
Department of Plastic and Reconstructive Surgery, Bowman Gray School of Medicine, Winston-Salem, North Carolina 27157-1075.
Duell
E A
EA
Jennings
D A
DA
Ledbetter
M S
MS
White
W L
WL
Argenta
L C
LC
D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; D000653:Amniotic Fluid / Q000737:chemistry / Q000502:physiology; D000818:Animals; D001095:Arachidonic Acids / Q000378:metabolism*; D015237:Dinoprost / Q000032:analysis; D015232:Dinoprostone / Q000032:analysis; D005260:Female; D005333:Fetus / Q000378:metabolism / Q000473:pathology; D005865:Gestational Age; D006893:Hydroxyeicosatetraenoic Acids / Q000032:analysis; D007249:Inflammation; D015289:Leukotrienes / Q000032:analysis; D009779:Occlusive Dressings; D011247:Pregnancy; D049188:Prenatal Injuries; D011817:Rabbits / Q000196:embryology; D012828:Silicones; D013537:Sutures; D014945:Wound Healing; D014950:Wounds, Penetrating / Q000196:embryology* / Q000378:metabolism / Q000473:pathology / Q000628:therapy
D001095:Arachidonic Acids; D006893:Hydroxyeicosatetraenoic Acids; D015289:Leukotrienes; D012828:Silicones; D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; C025984:15-hydroxy-5,8,11,13-eicosatetraenoic acid; D015237:Dinoprost; D015232:Dinoprostone