title: A fast method for processing biologic material for electron microscopic diagnosis in infectious disease.
abstract: A fast method for processing biologic material for electron microscopy for precise and specific diagnosis of infectious agents is an increasing necessity. After different, reportedly fast methods were tested, a useful and quick technique was developed that provides well-preserved cellular structures, enabling the etiologic diagnosis of infectious agents even in necrotic tissue or other biologic material such as sputum, bronchoalveolar lavage, and the like. This procedure takes less than 3 hours.
authors.affiliation: Department of Pathology, University of São Paulo Medical School, Brazil.
authors.forename: M I
authors.initials: MI
authors.lastname: Duarte
authors.forename: O N
authors.initials: ON
authors.lastname: Mariano
authors.forename: C F
authors.initials: CF
authors.lastname: Takakura
authors.forename: D
authors.initials: D
authors.lastname: Everson
authors.forename: C E
authors.initials: CE
authors.lastname: Corbett
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000150:complications; D001992:Bronchoalveolar Lavage Fluid / Q000382:microbiology / Q000469:parasitology; D016591:Histocytological Preparation Techniques; D006801:Humans; D007239:Infections / Q000175:diagnosis*; D007420:Intestine, Large / Q000382:microbiology / Q000469:parasitology / Q000648:ultrastructure; D008099:Liver / Q000382:microbiology / Q000469:parasitology / Q000648:ultrastructure; D008168:Lung / Q000382:microbiology / Q000469:parasitology / Q000648:ultrastructure; D008198:Lymph Nodes / Q000382:microbiology / Q000469:parasitology / Q000648:ultrastructure; D008854:Microscopy, Electron; D011020:Pneumonia, Pneumocystis / Q000150:complications / Q000175:diagnosis; D013183:Sputum / Q000382:microbiology / Q000469:parasitology; D014123:Toxoplasmosis / Q000150:complications / Q000175:diagnosis