Polymerase chain reaction-based DNA fingerprinting in the spontaneously hypertensive rat: potential source of cDNA library probes.
1. Using the Polymerase Chain Reaction with a primer homologous to a repetitive unit found at irregular distances in the rat genome, we were able to synthesize DNA fragments spanning such units and visualize them as a ladder of discrete bands following electrophoresis. 2. Differences between fingerprint patterns generated using SHR, WKY and SD DNA, in the form of band deletions or duplications, were readily detected. 3. Unlike traditional fingerprinting, PCR fingerprints allow the ready extraction and characterization of polymorphic bands. Such bands could then be used as probes with which to screen a cDNA library that might, in turn, identify expressed genes involved in the development of hypertension.
Di Nicolantonio
R
R
Department of Physiology, University of Melbourne, Parkville, Victoria, Australia.
Imai
T
T
Ikeda
K
K
Sawamura
M
M
Yamori
Y
Y
Murakami
K
K
D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000235:genetics; D016172:DNA Fingerprinting / Q000379:methods*; D015342:DNA Probes; D015723:Gene Library; D016678:Genome; D006973:Hypertension / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D051381:Rats / Q000235:genetics*; D011918:Rats, Inbred SHR / Q000235:genetics*; D011919:Rats, Inbred Strains; D011921:Rats, Inbred WKY; D012689:Sequence Homology, Nucleic Acid
D015342:DNA Probes; D004247:DNA