title: Monoclonal antibodies against metallothioneins from the human liver.
abstract: Six hybridomas secreting monoclonal antibodies directed against human fetal liver metallothioneins (MTs) have been generated and characterized. One antibody, K5A6, was specific for MT-1, the others recognized all isoMTs present in the human fetal liver. Each of these antibodies showed a unique cross-reactivity pattern when tested with MTs from the livers of different mammals. A double-antibody sandwich enzyme-linked-immunosorbent-assay (ELISA) was developed with the antibodies L2E3 and L5G2. This assay allows the detection of 60 pg human fetal liver MT and exhibits a metal dependent response for Zn-, Cd- and Hg-MT.
authors.affiliation: Katholieke Universiteit Leuven, Laboratorium voor Biochemie, Belgium.
authors.forename: K
authors.initials: K
authors.lastname: Van Houdt
authors.forename: I
authors.initials: I
authors.lastname: Nicasi
authors.forename: E
authors.initials: E
authors.lastname: Van Mechelen
authors.forename: B
authors.initials: B
authors.lastname: Veulemans
authors.forename: M
authors.initials: M
authors.lastname: De Ley
mesh_terms: D000328:Adult; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D003429:Cross Reactions; D004285:Dogs / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007668:Kidney / Q000737:chemistry; D008099:Liver / Q000737:chemistry* / Q000196:embryology; D008668:Metallothionein / Q000145:classification / Q000276:immunology* / Q000302:isolation & purification; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology; D010215:Papio / Q000276:immunology; D011817:Rabbits / Q000276:immunology; D051381:Rats / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D008668:Metallothionein