Microdetermination of G6PD isoenzyme activity in human erythrocytes by thin-layer PAG-IEF.
An improved method of microdetermination of G6PD isoenzyme activity in human erythrocytes was developed by modification of previously reported procedures. The volume of blood samples was reduced from 2 ml to 20 microliters. After hemolysis in 2% Triton X-100 and 0.1% beta-mercaptoethanol, the samples were subjected to centrifugation and thin-layer isoelectric focusing in polyacrylamide gel (PAG-IEF). By comparison with the original method, excellent resolution was obtained by this more rapid and simple procedure.
Nie
C
C
Hunan Medical University, Changsha, China.
Zhao
S
S
Lu
Y
Y
D000180:Acrylic Resins; D002855:Chromatography, Thin Layer / Q000379:methods; D004912:Erythrocytes / Q000201:enzymology*; D005954:Glucosephosphate Dehydrogenase / Q000097:blood*; D006801:Humans; D007525:Isoelectric Focusing / Q000379:methods; D007527:Isoenzymes / Q000097:blood*; D008832:Microchemistry / Q000379:methods
D000180:Acrylic Resins; D007527:Isoenzymes; C016680:polyacrylamide gels; D005954:Glucosephosphate Dehydrogenase