Platelet-derived growth factor in combination with collagen promotes the migration of human skin fibroblasts into a denuded area of a cell monolayer.
Since we have found previously that adult donor skin fibroblasts (TIG-114) migrated more slowly in serum-depleted medium than in medium supplemented with 10% FBS, we tried to identify a factor(s) which promotes fibroblast migration from the edge of a denuded area in a monolayer. In medium supplemented with 10% FBS, the effects of both suramin, a competitor of growth factors at the receptor level, and monensin, an inhibitor of the secretion of extracellular matrix, were examined. Both substances suppressed cell migration, suggesting that growth factors and matrix substances are important for cell migration. Then, we examined the effects of growth factors and extracellular matrix on fibroblast migration in serum-free medium. Platelet-derived growth factor (PDGF), basic fibroblast growth factor, acidic fibroblast growth factor, and transforming growth factor-beta did not stimulate cell migration. Type I collagen, plasma fibronectin, and heparin also did not promote cell migration. However, the combination of PDGF and type I collagen did promote cell migration. Addition of anti-PDGF antibody reduced the stimulatory effect induced by the combination of PDGF and type I collagen. These results suggest that the copresence of growth factors and extracellular matrix regulates fibroblast migration into a denuded area in a monolayer.
Kondo
H
H
Department of Experimental Biology, Tokyo Metropolitan Institute of Gerontology, Japan.
Matsuda
R
R
Yonezawa
Y
Y
D000328:Adult; D000906:Antibodies; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects*; D003094:Collagen / Q000494:pharmacology*; D004347:Drug Interactions; D005109:Extracellular Matrix / Q000502:physiology; D005347:Fibroblasts; D005353:Fibronectins / Q000494:pharmacology; D006133:Growth Substances / Q000494:pharmacology*; D006801:Humans; D007700:Kinetics; D008985:Monensin / Q000494:pharmacology; D010982:Platelet-Derived Growth Factor / Q000276:immunology / Q000494:pharmacology*; D012867:Skin; D013498:Suramin / Q000494:pharmacology
D000906:Antibodies; D005353:Fibronectins; D006133:Growth Substances; D010982:Platelet-Derived Growth Factor; D013498:Suramin; D003094:Collagen; D008985:Monensin