Mammalian skeletal muscle fibers distinguished by contents of phosphocreatine, ATP, and Pi.
We tested the proposition that muscle cell types have different contents of phosphocreatine (PCr), ATP, and Pi by 31P NMR spectroscopy and HPLC analyses of adult rat and mouse muscles containing various volume fractions of different fiber types. There was a 2-fold difference in the PCr content between muscles with a high volume fraction of fiber types 1 and 2x versus those with fast-twitch (types 2a and 2b) fiber types. Pi content was low, and PCr and ATP contents were high in muscles with large contents of type 2b and 2a fibers; the reverse was true in muscles with a large volume fraction of type 1 and 2x fibers. There is a large range in the Pi/PCr ratios in normal resting muscles, from less than 0.05 in type 2 to 0.51 in type 1 fibers, depending upon the distribution of their component fiber types. In all muscles, the peak area resulting from the beta phosphate of ATP constituted approximately 13% of the sum of all peak areas observable in the 31P spectrum. Fiber types 2a and 2b were not distinguishable, and the content of type 2x fibers was similar to type 1 fibers. From the profile of these metabolites, we could distinguish only two classes of fibers. For type 2a and 2b fibers, the intracellular concentrations were 8 mM ATP, 39 mM total creatine, 32 mM PCr, 0.8 mM Pi, and 8 microM ADP. For type 1 and 2x fibers, these quantities were 5 mM ATP, 23 mM total creatine, 16 mM PCr, 6 mM Pi, and 11 microM ADP. Thus our results establish an additional criterion upon which to distinguish skeletal muscle cells, one based on the resting content of bioenergetically important metabolites. These results also provide the basis for estimating skeletal muscle fiber-type composition from noninvasive NMR spectroscopic data.
Kushmerick
M J
MJ
Department of Radiology, University of Washington, Seattle 98105.
Moerland
T S
TS
Wiseman
R W
RW
D000255:Adenosine Triphosphate / Q000032:analysis*; D000818:Animals; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008433:Mathematics; D051379:Mice; D009132:Muscles / Q000378:metabolism*; D009928:Organ Specificity; D010710:Phosphates / Q000032:analysis*; D010725:Phosphocreatine / Q000032:analysis*; D010758:Phosphorus; D051381:Rats; D011919:Rats, Inbred Strains; D012044:Regression Analysis
D010710:Phosphates; D010725:Phosphocreatine; D010758:Phosphorus; D000255:Adenosine Triphosphate