Polyoxyethylene 9-lauryl ether-solubilized alkaline phosphatase: synergistic stimulation by zinc and magnesium ions.
1. Polidocanol-solubilized apoalkaline phosphatase could be stimulated either by zinc ions (Kd = 8.5 nM) or by magnesium ions alone (Kd = 3.8 microM). 2. Zinc and magnesium ions had synergistic effects on Polidocanol-solubilized apoalkaline phosphatase, leading to a fully active enzyme (700-800 U/mg). 3. Zinc ions inhibited non-competitively the Polidocanol-solubilized apoenzyme (Ki = 7.1 microM) by displacing magnesium ions from their binding sites. 4. A model for the action of zinc and magnesium ions on the modulation of the enzyme activity is proposed.
Ciancaglini
P
P
Departamento de Química, Faculdade de Filosofia, Ciências e Letras, Ribeirão Preto, Brazil.
Pizauro
J M
JM
Leone
F A
FA
D000469:Alkaline Phosphatase / Q000737:chemistry / Q000187:drug effects*; D000818:Animals; D003902:Detergents; D007700:Kinetics; D008274:Magnesium / Q000494:pharmacology*; D000077423:Polidocanol; D011092:Polyethylene Glycols; D051381:Rats; D012995:Solubility; D015032:Zinc / Q000494:pharmacology*
D003902:Detergents; D000077423:Polidocanol; D011092:Polyethylene Glycols; D000469:Alkaline Phosphatase; D008274:Magnesium; D015032:Zinc