title: Transcription of glpT of Escherichia coli K12 is regulated by anaerobiosis and fnr.
abstract: The transcriptional expression of the sn-glycerol 3-phosphate transport gene, glpT, was studied with a glpT-lac fusion contained on a low-copy-number plasmid pFZY1. The fusion was isolated during a 'shot gun' cloning of promoters expressed under anaerobic growth conditions. It was shown that glpT was induced six-fold by anaerobiosis. Furthermore, aerobic expression of glpT was induced by the substrate glycerol 3-phosphate, while the anaerobic expression of glpT was decreased by nitrate, glucose and a fnr mutation. However, nitrate repression on glpT expression was relieved if the upstream region from -290 of the transcription site was removed.
authors.affiliation: Department of Biology, Chinese University of Hong Kong, Shatin.
authors.forename: K K
authors.initials: KK
authors.lastname: Wong
authors.forename: H S
authors.initials: HS
authors.lastname: Kwan
mesh_terms: D000693:Anaerobiosis; D001426:Bacterial Proteins / Q000235:genetics*; D001693:Biological Transport, Active; D002352:Carrier Proteins / Q000096:biosynthesis*; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial / Q000502:physiology*; D005994:Glycerophosphates / Q000235:genetics*; D007506:Iron-Sulfur Proteins; D010957:Plasmids; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000235:genetics; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins; D002352:Carrier Proteins; D029968:Escherichia coli Proteins; C060704:FNR protein, E coli; D005994:Glycerophosphates; D007506:Iron-Sulfur Proteins; D011993:Recombinant Fusion Proteins; C021688:UgpB protein, E coli; C029620:alpha-glycerophosphoric acid