Effect of human IgG1 peptides on the antigen-specific antibody response of mice in vivo.
The effect of synthetic peptides--corresponding to the amino acid sequences 289-301 (Y48) and 293-301 (Y91) within the CH-2 domain in the human IgG1 was studied on the oxazolone-specific primary and secondary antibody response isotype distribution and on the sheep erythrocyte (SRBC)-specific primary IgM response. High responder (Balb/c) and low responder (C57Bl/6) mice to oxazolone hapten were treated intraperitoneally with various doses of peptides simultaneously with the first and second contact sensitization. The relative levels of oxazolone-specific IgM, IgG3, IgG1, IgG2a and IgG2b antibodies were determined by a solid phase radioimmunoassay. Y48 and Y91 peptides in a dose range of 10(-5) - 10(-8) M/animal enhanced the oxazolone-specific antibody response. This effect was more striking under suboptimal conditions: using smaller antigen dose for sensitization, cyclophosphamide pretreatment or using genetically low responder mice. SRBC-specific primary IgM response was enhanced by Y91 peptide, Y48 was ineffective.
Kádár
J
J
Second Department of Medicine, Semmelweis University, Budapest, Hungary.
Sármay
G
G
Rajnavölgyi
E
E
Stanworth
D
D
Gergely
P
P
D000818:Animals; D000917:Antibody Formation / Q000187:drug effects*; D000941:Antigens; D004912:Erythrocytes / Q000276:immunology; D005260:Female; D006801:Humans; D007141:Immunoglobulin Fc Fragments / Q000008:administration & dosage; D007074:Immunoglobulin G / Q000008:administration & dosage*; D007132:Immunoglobulin Isotypes / Q000096:biosynthesis; D007075:Immunoglobulin M / Q000096:biosynthesis; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D010081:Oxazolone / Q000276:immunology; D010446:Peptide Fragments / Q000276:immunology / Q000494:pharmacology*; D012756:Sheep
D000941:Antigens; D007141:Immunoglobulin Fc Fragments; D007074:Immunoglobulin G; D007132:Immunoglobulin Isotypes; D007075:Immunoglobulin M; D010446:Peptide Fragments; D010081:Oxazolone