title: Specific binding between human neutrophils and heparin.
abstract: Heparin binding on polymorphonuclear leucocytes (PMNL) was characterized. Heparin binding was specific, rapid, saturable and reversible. One single class of heparin binding sites was found with a dissociation constant of 1.22 mumol/l and 7.7 x 10(6) sites per PMNL. The binding was independent of the anticoagulant activity of heparin. Heparin affinity chromatography on radio-iodinated cell lysates followed by polyacrylamide gel electrophoresis in the presence of sodium dodecylsulphate revealed a 130 kD heparin binding protein. Heparin binding was inhibited by disodium salt of ethylenediamine tetraacetic acid. Cell surface bound heparin was functionally inactive and did not affect the inactivation of thrombin by antithrombin III. Our study demonstrates that heparin interacts with PMNL by a cell-surface binding protein. These instructions could be consistent with the modifications of some PMNL functional properties in the presence of heparin.
authors.affiliation: Laboratoire de Biochimie B, Hôpital Edouard Herriot, Lyon, France.
authors.forename: C
authors.initials: C
authors.lastname: Leculier
authors.forename: O
authors.initials: O
authors.lastname: Benzerara
authors.forename: N
authors.initials: N
authors.lastname: Couprie
authors.forename: A
authors.initials: A
authors.lastname: Francina
authors.forename: Y
authors.initials: Y
authors.lastname: Lasne
authors.forename: E
authors.initials: E
authors.lastname: Archimbaud
authors.forename: D
authors.initials: D
authors.lastname: Fiere
mesh_terms: D000990:Antithrombin III / Q000494:pharmacology; D001345:Autoradiography; D002846:Chromatography, Affinity; D004492:Edetic Acid / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D006493:Heparin / Q000378:metabolism*; D006801:Humans; D009504:Neutrophils / Q000378:metabolism*; D013462:Sulfur Radioisotopes; D013917:Thrombin / Q000378:metabolism
chemical_list: D013462:Sulfur Radioisotopes; D000990:Antithrombin III; D006493:Heparin; D004492:Edetic Acid; D013917:Thrombin