Use of a novel agarose gel-digesting enzyme for easy and rapid purification of PCR-amplified DNA for sequencing.
The use of a novel gel-digesting enzyme preparation provides an easy, rapid and convenient method to quantitatively recover PCR-amplified DNA from low melting point agarose gels. The PCR products purified using this method were readily sequenced and yielded good and unambiguous sequence data.
Gold
P
P
Gold Biotechnology, St. Louis, MO 63143.
D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000302:isolation & purification*; D004269:DNA, Bacterial; D006026:Glycoside Hydrolases / Q000378:metabolism*; D051379:Mice; D016133:Polymerase Chain Reaction / Q000379:methods*; D012685:Sepharose / Q000378:metabolism*; D013997:Time Factors
D004269:DNA, Bacterial; D004247:DNA; D012685:Sepharose; D006026:Glycoside Hydrolases; C019624:agarase