Parenteral administration of trypsin triggers lung emphysema.
Eight weeks after a single intravenous injection of trypsin, more than half of 26 treated rats showed pulmonary emphysema, as demonstrated by a significant increase of the mean linear intercept (MLI = 107 microns) in comparison with 11 controls (69 +/- 15 microns) (mean +/- SD). As observed 56 days after the injection, the intraperitoneal administration of trypsin (24 rats) also leads to lung emphysema (MLI = 101-106 microns), as does endotracheal instillation of elastase (13 rats), (MLI = 108 microns). The intraperitoneal administration of trypsin in animals constitutes a model close to human pathology with which lung alterations in acute pancreatitis may be studied. Having no elastolytic properties, trypsin cannot directly induce emphysema. The observation of a pulmonary leucostasis in eight rats sacrificed early after the trypsin injection suggested that leucocyte trapping and activation are important for the genesis of this trypsin-triggered emphysema.
Reichart
E
E
Unité 14 de l'INSERM, Vandoeuvre-les-Nancy, France.
Boerkmann
P
P
Plenat
F
F
D000208:Acute Disease; D000818:Animals; D007274:Injections, Intraperitoneal; D007275:Injections, Intravenous; D008168:Lung / Q000473:pathology; D008297:Male; D010196:Pancreatic Elastase / Q000009:adverse effects; D010195:Pancreatitis / Q000473:pathology; D011656:Pulmonary Emphysema / Q000139:chemically induced* / Q000473:pathology; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors; D014357:Trypsin / Q000008:administration & dosage / Q000009:adverse effects*
D010196:Pancreatic Elastase; D014357:Trypsin