title: Enzyme-catalyzed oligosaccharide synthesis.
abstract: Cell-surface carbohydrates and their conjugates are involved in many types of molecular recognition. This review describes recent developments in enzyme-catalyzed oligosaccharide synthesis, with particular focus on glycosyltransferase and glycosidase reactions. With the increasing availability of glycosyltransferases via recombinant DNA technology, glycosyltransferase-catalyzed glycosylation with in situ regeneration of sugar nucleotides appears to be the most effective method for large-scale stereocontrolled oligosaccharide synthesis.
authors.affiliation: Department of Chemistry, Scripps Research Institute, La Jolla, California 92037.
authors.forename: Y
authors.initials: Y
authors.lastname: Ichikawa
authors.forename: G C
authors.initials: GC
authors.lastname: Look
authors.forename: C H
authors.initials: CH
authors.lastname: Wong
mesh_terms: D002240:Carbohydrate Sequence; D006026:Glycoside Hydrolases / Q000378:metabolism*; D016695:Glycosyltransferases / Q000378:metabolism*; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000096:biosynthesis*
chemical_list: D009844:Oligosaccharides; D016695:Glycosyltransferases; D006026:Glycoside Hydrolases