title: The effect of corticotropin-releasing factor (CRF) on autonomic and behavioral responses during shock-prod burying test in rats.
abstract: When administered intracerebroventricularly (ICV) in rats, corticotropin-releasing factor (CRF) possesses arousing and anxiogenic properties, which may be found reflected in autonomic and behavioral activation. As these responses are dependent on dose and situation, ICV-injected CRF may affect behavioral responses to a defined stimulus in a different fashion than autonomic concomitants. Two experiments were conducted in order to test this hypothesis. In both experiments, rats were treated ICV with CRF or an artificial cerebrospinal fluid (aCSF) 5 min prior to a 15-min exposure to an electrified prod (shock-prod burying test, SPB test) in their home cages. In the first experiment, 0.3 ng CRF injected ICV in unhandled rats significantly reduced the prod-burying response to electric shock, in favor of immobility, whereas following 300 ng CRF ICV, the predominant behavioral response was grooming behavior. In contrast, habituated rats, implanted with telemetric devices to measure heart rate, core temperature, and gross activity in the second experiment, showed a significant increase of burying behavior after 0.3 ng CRF ICV, in comparison to vehicle-treated controls. However, simultaneous cardiac acceleration was of the same magnitude and duration in both groups. In addition, whereas similar rises in CT were observed in both groups during the SPB test, CRF-treated rats showed more marked rise in core temperature during the first 15 min of the posttest period. At the 24-h retention test, rats belonging to the CRF group showed burying behavior and HR responses, in onset, magnitude, and duration similar to day 1, whereas extinction of the burying response and tachycardia was found in controls. Changes in CT, although less marked, showed the same pattern as on day 1 in both groups. These results show a differential effect of central CRF on behavioral and autonomic activation induced by a well-defined stressful stimulus. The response to CRF seems to be not only situation related, but also dependent on the pretest experience of the animal.
authors.affiliation: Rudolf Magnus Institute, Medical Faculty, University of Utrecht, The Netherlands.
authors.forename: M
authors.initials: M
authors.lastname: Diamant
authors.forename: G
authors.initials: G
authors.lastname: Croiset
authors.forename: D
authors.initials: D
authors.lastname: de Wied
mesh_terms: D000818:Animals; D001341:Autonomic Nervous System / Q000187:drug effects*; D001362:Avoidance Learning / Q000187:drug effects*; D001831:Body Temperature / Q000187:drug effects; D002404:Catheterization; D003346:Corticotropin-Releasing Hormone / Q000494:pharmacology*; D004597:Electroshock; D006234:Handling, Psychological; D006339:Heart Rate / Q000187:drug effects; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012153:Retention, Psychology / Q000187:drug effects; D013312:Stress, Physiological / Q000188:drug therapy* / Q000503:physiopathology
chemical_list: D003346:Corticotropin-Releasing Hormone