title: Construction of a tRNA-embedded-ribozyme trimming plasmid.
abstract: We have combined Cotten and Birnstiel's tRNA-embedded ribozymes and our 5'- and 3'-trimming system. Although the activity of the tRNA-embedded ribozyme was ca. 30% lower than those of naked ribozymes, since the stability of the former in bovine serum was higher than those of naked ribozymes, the tRNA-embedded ribozymes appear useful especially when the 5'- and 3'-trimming units are concatenated in tandem.
authors.affiliation: Fermentation Research Institute, Agency of Industrial Science & Technology, MITI, Tsukuba Science City, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Yuyama
authors.forename: J
authors.initials: J
authors.lastname: Ohkawa
authors.forename: Y
authors.initials: Y
authors.lastname: Inokuchi
authors.forename: M
authors.initials: M
authors.lastname: Shirai
authors.forename: A
authors.initials: A
authors.lastname: Sato
authors.forename: S
authors.initials: S
authors.lastname: Nishikawa
authors.forename: K
authors.initials: K
authors.lastname: Taira
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000235:genetics* / Q000378:metabolism; D005821:Genetic Techniques; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides; D010957:Plasmids; D016337:RNA, Catalytic / Q000378:metabolism*; D012343:RNA, Transfer / Q000235:genetics / Q000378:metabolism*; D015183:Restriction Mapping; D013698:Templates, Genetic
chemical_list: D009838:Oligodeoxyribonucleotides; D016337:RNA, Catalytic; D004247:DNA; D012343:RNA, Transfer