Specific release of a 9-kDa extrinsic polypeptide of photosystem I from spinach chloroplasts by salt washing.
The newly reported 9-kDa polypeptide in photosystem I [(1991) FEBS Lett. 280, 332-334] is an extrinsic component located on the lumenal side of the thylakoid membrane. This subunit can be solubilized with high salt buffer and does not bind any cofactors. The photosystem I electron transfer chain remains intact and functional in the absence of this component as characterized by the photoreduction of NADP+.
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Department of Plant Biology, University of California, Berkeley 94720.
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D000595:Amino Acid Sequence; D002736:Chloroplasts / Q000737:chemistry* / Q000378:metabolism; D004579:Electron Transport; D007425:Intracellular Membranes / Q000737:chemistry / Q000378:metabolism; D008565:Membrane Proteins / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D009249:NADP / Q000378:metabolism; D045322:Photosynthetic Reaction Center Complex Proteins / Q000737:chemistry* / Q000378:metabolism; D045331:Photosystem I Protein Complex; D010940:Plant Proteins / Q000302:isolation & purification*; D010944:Plants; D010970:Plastocyanin / Q000378:metabolism
D008565:Membrane Proteins; D045322:Photosynthetic Reaction Center Complex Proteins; D045331:Photosystem I Protein Complex; D010940:Plant Proteins; D009249:NADP; D010970:Plastocyanin