The stability of 99Tcm directly labelled to an Fab' antibody via stannous ion and mercaptoethanol reduction.
The anti-CEA FO23C5 F(ab')2 antibody was directly radiolabelled with 99mTcm by two methods (stannous ion and mercaptoethanol reduction) and compared in vitro and in vivo for label stability. By both methods, reduction of the F(ab')2 fragment produced primarily Fab' fragments. By both methods, the label was stable to 99Tcm-pertechnetate formation in vitro. Analysis by high performance liquid chromatography (HPLC) of serum, urine, kidney and liver homogenates from mice injected with 99Tcm-antibodies by both methods consistently showed a prominent radiolabelled peak with an estimated molecular weight of about 300 daltons. An identical peak was observed in the analysis of patient samples in a related investigation from this laboratory. Cysteine was radiolabelled with reduced 99Tcm and analysed by HPLC and thin layer chromatography (TLC); one of the 99Tcm-cysteine species so produced showed the same chromatographic behaviour as that of the 300 dalton species. In conclusion, the FO23C5 and other antibodies are stably labelled with 99Tcm via either stannous ion or mercaptoethanol reduction. In mice and in patients, the labelled proteins are either catabolized or, more likely, the 99Tcm label is transchelated such that the label is present on several low molecular weight species, the most prominent of which is postulated to be 99Tcm-cysteine.
Mardirossian
G
G
Department of Nuclear Medicine, University of Massachusetts Medical Center, Worcester 01655.
Wu
C
C
Rusckowski
M
M
Hnatowich
D J
DJ
D000818:Animals; D004355:Drug Stability; D006801:Humans; D007140:Immunoglobulin Fab Fragments; D007477:Ions; D007553:Isotope Labeling / Q000379:methods*; D008297:Male; D008623:Mercaptoethanol; D051379:Mice; D010084:Oxidation-Reduction; D013667:Technetium; D014001:Tin
D007140:Immunoglobulin Fab Fragments; D007477:Ions; D008623:Mercaptoethanol; D013667:Technetium; D014001:Tin