Low-temperature fluorometric technique for evaluating the viability of rat liver grafts after simple cold storage.
Time-dependent changes in the viability of rat liver graft during cold preservation with Euro-Collins solution were evaluated with NADH fluorometry. Correlation between the fluorometric analysis, 1-week survival rate after liver transplantation, and mitochondrial ATP synthesis activity in the early phase after transplantation was studied. Fluorometric study: Rat livers were preserved at 0 degree-4 degrees C for 0-48 h in Euro-Collins solution and then reperfused for 15 min with oxygenated Krebs-Henseleit solution at 4 degrees C. The amplitude (R x A) between the oxidized and the reduced steady-state NADH fluorometric trace and the velocity (R x V) of the trace were determined to evaluate the mitochondrial respiratory chain. The R x A and R x V remained at levels higher than 90% of control after 6-h preservation, while the R x A of the 9-h preservation group and the R x V of the 12-h preservation group decreased significantly compared with those of the control and the 6-h preservation group. Survival study: a 100% survival rate after transplantation was achieved in the 6-h preservation group, whereas the rates were 18.8% and 0% in the 9- and 12-h preservation groups respectively. These survival rates correlated closely with the time-dependent decrease of the fluorometric parameters. Study of mitochondrial phosphorylative activity and energy charge 3 h after transplantation: With fresh grafts, the decrease in hepatic energy charge after transplantation was reduced to 0.79 from the control value of 0.86 by a 30% increase in mitochondrial ATP synthesis ability.(ABSTRACT TRUNCATED AT 250 WORDS)
Okamura
R
R
Second Department of Surgery, Faculty of Medicine, Kyoto University, Japan.
Tanaka
A
A
Uyama
S
S
Ozawa
K
K
D000255:Adenosine Triphosphate / Q000096:biosynthesis; D000818:Animals; D003080:Cold Temperature; D005069:Evaluation Studies as Topic; D005470:Fluorometry / Q000379:methods*; D006085:Graft Survival / Q000502:physiology; D066298:In Vitro Techniques; D016031:Liver Transplantation / Q000502:physiology*; D008297:Male; D008930:Mitochondria, Liver / Q000378:metabolism; D009926:Organ Preservation / Q000379:methods*; D010085:Oxidative Phosphorylation; D051381:Rats; D011917:Rats, Inbred Lew
D000255:Adenosine Triphosphate