[Plasma-membrane fluidity studies of murine neuroblastoma and malignant melanoma cells under irradiation].
Murine neuroblastoma (C-1300 NMB) and malignant melanoma (B16) cells were radiated in presence of radiopharmaceutics. Sensibilization was carried out with BSO and protection with TMX. Changes in fluidity of the plasma membrane, in cellular GSH contents and cell cycle were observed. After radiation fluidity of the plasma membrane is increased, whereas intracellular GSH decreased. These changes were intensified by BSO and reduced by TMX. Fluidity of the plasma membrane correlates with intracellular GSH and also with cell cycle. It is suggested that changes in plasma membrane fluidity can be used as an additional parameter for the determination of sensitivity towards radiation.
Busse
E
E
Abteilung für Hämatologie und Onkologie, J.-W.-Goethe-Universität, Frankfurt/Main.
Zimmer
G
G
Kornhuber
B
B
D000818:Animals; D019328:Buthionine Sulfoximine; D002453:Cell Cycle / Q000187:drug effects / Q000528:radiation effects; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D005978:Glutathione / Q000187:drug effects / Q000528:radiation effects; D008546:Melanoma, Experimental / Q000532:radiotherapy* / Q000648:ultrastructure; D008560:Membrane Fluidity / Q000187:drug effects / Q000528:radiation effects*; D008717:Methionine Sulfoximine / Q000031:analogs & derivatives / Q000494:pharmacology; D051379:Mice; D009447:Neuroblastoma / Q000532:radiotherapy* / Q000648:ultrastructure; D011836:Radiation Tolerance / Q000187:drug effects; D011837:Radiation-Protective Agents / Q000494:pharmacology; D011838:Radiation-Sensitizing Agents / Q000494:pharmacology; D013629:Tamoxifen / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000528:radiation effects
D011837:Radiation-Protective Agents; D011838:Radiation-Sensitizing Agents; D013629:Tamoxifen; D008717:Methionine Sulfoximine; D019328:Buthionine Sulfoximine; D005978:Glutathione
Untersuchungen über die Fluidität der Plasmamembran von murinen Neuroblastom- und malignen Melanomzellen unter Bestrahlung.