Chromatographic separation of oligodeoxynucleotides with identical length: application to purification of oligomers containing a modified base.
A general procedure is described for separation and purification of oligodeoxynucleotides of identical length but different base composition, in particular, of oligomers containing modified bases such as 4-substituted thymines and 6-substituted guanines, using an anion-exchange column (either Mono Q or NucleoPac). The modified oligomers can be well separated from the analogous oligomers containing unmodified thymine or guanine under the basic conditions of the chromatography. The effects of oligomer length, base composition, and lipophilicity on the separation are discussed. A general rule which can be used for prediction of the order of elution of different oligomers and for estimation of tautomeric form of a modified base in the oligomer is presented.
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Department of Biochemistry and Molecular Biology, University College London, England.
Swann
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D000837:Anion Exchange Resins; D001482:Base Composition; D001483:Base Sequence; D002852:Chromatography, Ion Exchange / Q000379:methods*; D006147:Guanine / Q000031:analogs & derivatives; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000737:chemistry / Q000302:isolation & purification*; D011089:Polydeoxyribonucleotides / Q000737:chemistry / Q000302:isolation & purification; D012117:Resins, Synthetic; D013941:Thymine / Q000031:analogs & derivatives
D000837:Anion Exchange Resins; D009838:Oligodeoxyribonucleotides; D011089:Polydeoxyribonucleotides; D012117:Resins, Synthetic; D006147:Guanine; C042855:Mono Q; D013941:Thymine