Prolonged bleeding time of Chediak-Higashi cats corrected by platelet transfusion.
Cats with the Chediak-Higashi syndrome (CHS) have a platelet storage pool deficiency (SPD). Ten CHS cats were transfused with a concentrate of 51Cr-labeled platelets prepared from normal donor cats. One hour after transfusion, the donor platelet count in CHS recipient cats was 40,000-60,000/microliters. Bleeding time before transfusion was 9.1 +/- 3.0 min. When donor platelet count in CHS cats was 50,000/microliters, bleeding time was 1.7 +/- 0.2 min. Bleeding time of normal cats was 1.4 +/- 0.3 min. Bleeding time increased to 3.3 +/- 0.2 min and to 5.3 +/- 0.2 min when the platelet count was 30,000/microliters, and 15,000/microliters, respectively. The close inverse relationship between bleeding time and number of donor platelets in CHS cats (r = -0.92), suggests that prolonged bleeding time is due to a platelet abnormality, that platelet transfusion can effectively correct prolonged bleeding time in an animal model of platelet SPD and that CHS cats may be an appropriate animal model to evaluate hemostatic capabilities of transfused platelets.
Cowles
B E
BE
Department of Veterinary and Comparative Anatomy, Pharmacology and Physiology, Washington State University, Pullman 99164-6520.
Meyers
K M
KM
Wardrop
K J
KJ
Menard
M
M
Sylvester
D
D
D000818:Animals; D001760:Bleeding Time; D016913:Blood Component Transfusion; D002415:Cats; D002609:Chediak-Higashi Syndrome / Q000503:physiopathology / Q000628:therapy*; D002860:Chromium Radioisotopes; D004195:Disease Models, Animal; D009061:Mouth Mucosa / Q000503:physiopathology; D010974:Platelet Aggregation / Q000502:physiology; D010981:Platelet Storage Pool Deficiency / Q000503:physiopathology / Q000628:therapy*
D002860:Chromium Radioisotopes