(ADP-ribosyl)ation pattern of chromosomal proteins during ageing.
(ADP-ribosyl)ation of chromosomal proteins was studied by incubating the nuclei of brain and liver of young and old rats with 14C-NAD+. In brain as well as in liver histone proteins show approximately 2-3 fold higher (ADP-ribosyl)ation than that of non-histone chromosomal (NHC) proteins of both the age groups. H1 seems to be the major target for (ADP-ribosyl)ation. Amongst nucleosomal histones H2B is the main acceptor of 14C-labelled ADP-ribose moieties. A sharp age related decline of (ADP-ribosyl)ation of chromosomal proteins was observed in both the tissues.
Mishra
S K
SK
Molecular Biology and Genetic Engineering Unit, Institute of Life Sciences, Sahid Nagar, Bhubaneswar, India.
Das
B R
BR
D000375:Aging / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000378:metabolism; D006657:Histones / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D009687:Nuclear Proteins / Q000378:metabolism*; D011064:Poly Adenosine Diphosphate Ribose / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D051381:Rats; D011919:Rats, Inbred Strains
D006657:Histones; D009687:Nuclear Proteins; D011064:Poly Adenosine Diphosphate Ribose