[The role of transaminases in realizing the protective effect of L-aspartate in hypoxia].
Activation of aspartate aminotransferase and alanine aminotransferase of mitochondria introduced to the incubation medium of pyridoxal-5'-phosphate (40 microM) is approximately 2 times higher than that of the corresponding cytoplasmic forms. At hypoxia aspartate aminotransferase activity in mitochondria and postmitochondrial supernatant tends to an increase while that of alanine aminotransferase decreases (above 2 times). The protection from hypoxic damage when using L-aspartate (100 mg/kg subcutaneously 3-5 min before hypoxia) intensifies an adaptive increase of aspartate aminotransferase activity and removes a decrease of alanine aminotransferase activity. Under these conditions stimulating effect of pyridoxal-5'-phosphate on transaminases activity in vitro weakens. A simultaneous administration of vitamin-coenzyme complex (thiamine pyrophosphate, lipoate, sodium 4-phospho-pantothenate, flavin-mononucleotide, nicotinate) intensifies these metabolic shifts and protective action of L-aspartate.
Abu Asali
I I
II
Rozanov
V A
VA
Rozanov
A Ia
AIa
D000410:Alanine Transaminase / Q000378:metabolism*; D000818:Animals; D001219:Aspartate Aminotransferases / Q000378:metabolism*; D001224:Aspartic Acid / Q000494:pharmacology*; D000860:Hypoxia / Q000517:prevention & control*; D051379:Mice; D008928:Mitochondria / Q000187:drug effects / Q000201:enzymology; D009417:Nerve Tissue / Q000187:drug effects / Q000201:enzymology; D011732:Pyridoxal Phosphate / Q000494:pharmacology
D001224:Aspartic Acid; D011732:Pyridoxal Phosphate; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase
Rol' transaminaz v realizatsii zashchitnogo éffekta L-aspartata pri gipoksii.