Phosphorylation of FAR1 in response to alpha-factor: a possible requirement for cell-cycle arrest.
Exposure of yeast a cells to alpha-factor causes cells to arrest in the G1 phase of the cell cycle. The FAR1 gene is required for this cell-cycle arrest; its product is necessary for the inhibition of a G1 cyclin, CLN2. Earlier work demonstrated that alpha-factor caused an increase in the transcription of FAR1 severalfold over a measurable basal level. We now show that transcriptional induction of FAR1 from a heterologous promoter is not sufficient to inhibit CLN2 in the absence of alpha-factor. We also show that FAR1 is phosphorylated in response to alpha-factor and propose that this phosphorylation may be required for FAR1 activity.
Chang
F
F
Department of Biochemistry and Biophysics, University of California, San Francisco 94143-0448.
Herskowitz
I
I
D001483:Base Sequence; D002453:Cell Cycle / Q000187:drug effects / Q000502:physiology*; D005656:Fungal Proteins / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D000072235:Mating Factor; D008969:Molecular Sequence Data; D010455:Peptides / Q000494:pharmacology*; D010675:Pheromones / Q000494:pharmacology*; D010766:Phosphorylation; D011401:Promoter Regions, Genetic; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000502:physiology*; D014158:Transcription, Genetic
D005656:Fungal Proteins; D010455:Peptides; D010675:Pheromones; D000072235:Mating Factor