High-level resistance to ethidium bromide and antiseptics in Staphylococcus aureus.
A gene encoding high-level resistance to ethidium bromide (EB) and antiseptics was isolated from a transferable plasmid, pTZ22, in Staphylococcus aureus. DNA sequencing revealed that the plasmid has two copies of the ebr gene that normally mediates low-level resistance to EB and antiseptics. The efflux rate for EB of strains with duplicated ebr genes was twice the rate of strains with a single ebr gene. It was concluded that the duplication of ebr is responsible for the high-level resistance to EB and antiseptics.
Sasatsu
M
M
Department of Microbiology, Tokyo College of Pharmacy, Japan.
Shibata
Y
Y
Noguchi
N
N
Kono
M
M
D000595:Amino Acid Sequence; D000891:Anti-Infective Agents, Local / Q000494:pharmacology*; D001483:Base Sequence; D002872:Chromosome Deletion; D002876:Chromosomes, Bacterial; D004352:Drug Resistance, Microbial; D004996:Ethidium / Q000378:metabolism / Q000494:pharmacology*; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics; D013211:Staphylococcus aureus / Q000187:drug effects / Q000235:genetics*
D000891:Anti-Infective Agents, Local; D004996:Ethidium