Coregulation of purine and histidine biosynthesis by the transcriptional activators BAS1 and BAS2.
We have found cross-pathway regulation between purine and histidine biosynthesis in yeast. The transcription factors BAS1 and BAS2/PHO2, which are also regulators of the histidine pathway, participate in the regulation of the purine biosynthetic pathway. Analysis of four genes of the purine pathway (ADE1, ADE2, ADE5,7, and ADE8) shows that their expression is repressed by adenine. The maximal basal and induced expression of these purine genes requires the presence of both BAS1 and BAS2. The factor BAS1 has been shown to bind at a site containing the TGACTC hexanucleotide motif in the ADE2 and ADE5,7 promoters. This motif is required for both basal and induced activation of the ADE2 gene by BAS1 and BAS2.
Daignan-Fornier
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Whitehead Institute for Biomedical Research, Nine Cambridge Center, MA 02142-1479.
Fink
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D001483:Base Sequence; D001665:Binding Sites; D004271:DNA, Fungal / Q000235:genetics / Q000378:metabolism; D005656:Fungal Proteins / Q000378:metabolism*; D006639:Histidine / Q000096:biosynthesis*; D018398:Homeodomain Proteins; D007700:Kinetics; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D011401:Promoter Regions, Genetic; D011687:Purines / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D015534:Trans-Activators / Q000378:metabolism*; D014157:Transcription Factors; D001616:beta-Galactosidase / Q000235:genetics* / Q000378:metabolism
C061767:BAS1 protein, S cerevisiae; D004271:DNA, Fungal; D005656:Fungal Proteins; D018398:Homeodomain Proteins; D009838:Oligodeoxyribonucleotides; C051611:PHO2 protein, S cerevisiae; D011687:Purines; D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins; D015534:Trans-Activators; D014157:Transcription Factors; D006639:Histidine; D001616:beta-Galactosidase