UV-C irradiation-induced peroxidative degradation of microsomal fatty acids and proteins: protection by an extract of Ginkgo biloba (EGb 761).
After exposure of rat liver microsomes to UV-C irradiation, analysis of membrane fatty acids by gas chromatography confirmed that EGb 761, a drug containing a dosed and standardized extract of Ginkgo biloba, provides effective protection against free radical attack in vitro. This analysis, coupled with thiobarbituric acid (TBA) reaction, permitted qualitative and overall quantitative evaluation of radical-induced damage to polyunsaturated fatty acids (PUFA), as well as evidence of the antioxidant properties of the Ginkgo biloba extract. Assay of thiobarbituric acid reactive substances (TBARS) showed a correlation between TBARS concentration and the state of degradation of the polyunsaturated fatty acids. Mannitol (5.5 mM) did not prevent degradation of microsomal PUFA or malondialdehyde (MDA) production, nor did it prevent polymerization of membrane proteins. Low doses of EGb 761 were found to provide efficient protection of membrane PUFA regardless of individual susceptibility to peroxidation. This protection was accompanied by a decrease in the production of TBARS. EGb 761 also protected membrane proteins from the irreversible polymerization induced by these degradation products, but did not appear to prevent thiols oxidation into disulfide bonds.
Dumont
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Laboratoire de Biochimie, CNRS SDI 6129, Université de Caen, France.
Petit
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Tarrade
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Nouvelot
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A
D000818:Animals; D005227:Fatty Acids / Q000378:metabolism*; D005231:Fatty Acids, Unsaturated / Q000378:metabolism; D005609:Free Radicals; D020441:Ginkgo biloba; D007700:Kinetics; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism; D008862:Microsomes, Liver / Q000737:chemistry*; D010545:Peroxides / Q000378:metabolism*; D010936:Plant Extracts / Q000494:pharmacology*; D011506:Proteins / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013858:Thiobarbiturates; D014466:Ultraviolet Rays; D000096983:Ginkgo Extract
D005227:Fatty Acids; D005231:Fatty Acids, Unsaturated; D005609:Free Radicals; D008315:Malondialdehyde; D008565:Membrane Proteins; D010545:Peroxides; D010936:Plant Extracts; D011506:Proteins; D013858:Thiobarbiturates; C063170:Ginkgo biloba extract; D000096983:Ginkgo Extract; C029684:thiobarbituric acid