Regulation of nuclear transport of a plant potyvirus protein by autoproteolysis.
The NIa proteinase encoded by tobacco etch potyvirus catalyzes six processing events, three of which occur by an autoproteolytic mechanism. Autoproteolysis is necessary to cleave the boundaries of both NIa and the 6-kDa protein, which is located adjacent to the N terminus of NIa in the viral polyprotein. As a consequence, NIa may exist in a free form or in a transient polyprotein form containing the 6-kDa protein. While the majority of NIa molecules localize to the nuclei of infected cells, a fraction of the NIa pool is attached covalently to the 5' terminus of genomic RNA in the cytoplasm. To determine whether the presence of the 6-kDa protein affects the nuclear transport properties of NIa, we have generated transgenic plants that express genes encoding a reporter enzyme, beta-glucuronidase (GUS), fused to NIa or NIa-containing polyproteins. The NIa/GUS fusion protein was detected by histochemical analysis in the nucleus. Similarly, an NIa/GUS fusion protein that arose by autoproteolysis of a 6-kDa/NIa/GUS polyprotein was found in the nucleus. In contrast, fusion protein consisting of 6-kDa/NIa/GUS, which failed to undergo proteolysis because of the presence of a Cys-to-Ala substitution in the proteolytic domain of NIa, was detected in the cytoplasm. The inhibition of NIa-mediated nuclear transport was not due to the Cys-to-Ala substitution, since this alteration had no effect on translocation in the absence of the 6-kDa protein. These results indicate that the 6-kDa protein impedes nuclear localization of NIa and suggest that subcellular transport of NIa may be regulated by autoproteolysis.
Restrepo-Hartwig
M A
MA
Department of Biology, Texas A&M University, College Station 77843-3258.
Carrington
J C
JC
D001692:Biological Transport; D002467:Cell Nucleus / Q000378:metabolism*; D010450:Endopeptidases / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D005966:Glucuronidase / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification; D006651:Histocytochemistry; D010942:Plant Viruses / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D010947:Plants, Toxic; D011499:Protein Processing, Post-Translational; D011506:Proteins / Q000378:metabolism; D012328:RNA Viruses / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification; D014026:Nicotiana / Q000382:microbiology; D014764:Viral Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*
D011506:Proteins; D011993:Recombinant Fusion Proteins; D014764:Viral Proteins; D005966:Glucuronidase; D010450:Endopeptidases