In situ mapping of the gene coding for a leucine zipper DNA binding protein (CDR62) to 16p12-16p13.1.
A cDNA clone encoding the major antigen (CDR62) associated with the antibody-induced paraneoplastic cerebellar degeneration has been used to identify the chromosomal location of the corresponding structural gene(s) by screening for its retention in a panel of rodent-human somatic cell hybrids. Having established the synteny of the gene with the autosome 16, we proceeded to its precise subregional mapping by in situ fluorescence hybridization with a recombinant lambda phage containing the genomic region homologous to the cDNA gene. The latter studies pin-pointed the chromosomal localization of the structural CDR62 gene within the interval 16p12-16p13.1.
Gress
T
T
Imperial Cancer Research Fund, London, Great Britain.
Baldini
A
A
Rocchi
M
M
Furneaux
H
H
Posner
J B
JB
Siniscalco
M
M
D002871:Chromosome Banding; D002874:Chromosome Mapping; D002885:Chromosomes, Human, Pair 16; D004247:DNA; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D005453:Fluorescence; D005796:Genes; D006801:Humans; D016350:Leucine Zippers / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D015183:Restriction Mapping
D004268:DNA-Binding Proteins; D004247:DNA