NF1-related locus on chromosome 15.
A neurofibromatosis type I (NF1)-related locus has been identified on chromosome 15. It contains a partial copy of the NF1 GAP-related domain, which is known to interact with the ras protooncogenes. However, the chromosome 15 sequence contains multiple deletions resulting in frameshift mutations and stop codons in several highly conserved sequence blocks. The locus on chromosome 15 therefore represents an NF1 pseudogene. This nonprocessed NF1 pseudogene may produce additional fragments in Southern blotting, pulsed-field gel, and PCR experiments with some NF1 cDNA probes or oligonucleotides. In addition, certain regions of the NF1 gene also cross-hybridize with a locus on chromosome 14. These loci must be considered in mutation analysis of patients with NF1 since aberrant findings may not always reflect changes in the NF1 gene.
Legius
E
E
Department of Pediatrics, University of Michigan, Ann Arbor 48109.
Marchuk
D A
DA
Hall
B K
BK
Andersen
L B
LB
Wallace
M R
MR
Collins
F S
FS
Glover
T W
TW
D001483:Base Sequence; D015139:Blotting, Southern; D002872:Chromosome Deletion; D002874:Chromosome Mapping; D002884:Chromosomes, Human, Pair 15; D004247:DNA; D004591:Electrophoresis, Polyacrylamide Gel; D016368:Frameshift Mutation; D016514:Genes, Neurofibromatosis 1 / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D011544:Pseudogenes; D012689:Sequence Homology, Nucleic Acid
D004247:DNA