Spectrophotometric method of quantifying adult cat flea (Siphonaptera: Pulicidae) feces.
Cat flea, Ctenocephalides felis felis (Bouché), feces are an essential part of larval nutrition under natural conditions. The mass values of adult flea feces can be measured by dissolving samples of flea feces in Drabkin's reagent, filtering, centrifuging, and measuring absorbance spectrophotometrically at 540 nm. Either flea feces or air-dried host blood can be used to generate the standard curve used to convert absorbance (optical density) values into mass values. Debris collected from flea-infested house cats averaged 23.02 mg debris per cat per h with an average of 10.41 mg flea feces per cat per h. Flea feces deposited in the environment serve as potential larval food. Adult flea feces comprised an average of 44.28% of the debris deposited from infested domestic house cats in this study.
Kern
W H
WH
Department of Entomology and Nematology, University of Florida, Gainesville 32611.
Koehler
P G
PG
Patterson
R S
RS
Wadleigh
R W
RW
D000818:Animals; D002415:Cats; D003672:Defecation; D005243:Feces / Q000737:chemistry*; D006454:Hemoglobins / Q000032:analysis*; D005423:Siphonaptera / Q000502:physiology*; D013053:Spectrophotometry
D006454:Hemoglobins