[Kinetic characteristics of creatine kinase and hexokinase from rat skeletal muscles during dietary deficit of vitamin K and administration of pelentane].
Kinetic parameters of rat creatine kinase isozymes at different vitamin K supply and treatment with antivitamin K--pelentan have been determined. MM-isozyme (skeletal muscle) has selective sensitivity to the vitamin K deficit, while BB and MB-isozymes (brain, kidney and heart) have not. The value KM for ATP of MM-isozymes increases, while maximal activity decreases. Pelentan treatment does not lead to the change of MM-creatine kinase affinity to ATP. Soluble hexokinase of skeletal muscle in rats with vitamin K deficiency and treated with pelentan has higher affinity to glucose as compared to normal rat enzyme. It has been supposed that skeletal muscle hexokinase exists in a particular molecular form under vitamin K deficiency.
Sokol'nikov
A A
AA
Avramova
L V
LV
Kodentsova
V M
VM
Vrzhesinskaia
O A
OA
Goncharova
N Iu
NIu
Spirichev
V B
VB
D000818:Animals; D003402:Creatine Kinase / Q000378:metabolism*; D004032:Diet; D005017:Ethyl Biscoumacetate / Q000008:administration & dosage*; D006593:Hexokinase / Q000378:metabolism*; D007527:Isoenzymes; D007700:Kinetics; D009132:Muscles / Q000201:enzymology*; D051381:Rats; D011919:Rats, Inbred Strains; D014813:Vitamin K Deficiency / Q000378:metabolism*
D007527:Isoenzymes; D005017:Ethyl Biscoumacetate; D006593:Hexokinase; D003402:Creatine Kinase
Kineticheskie kharakteristiki kreatinkinazy i geksokinazy skeletnykh myshts krysy pri pishchevom defitsite vitamina K i vvedenii pelentana.