Screening and identification of a gene, PSE-1, that affects protein secretion in Saccharomyces cerevisiae.
Utilizing a screening method designed for the identification of genes involved with enhanced protein secretion in Saccharomyces cerevisiae we identified a gene, which we named PSE-1 (Protein Secretion Enhancer). Overexpression of PSE-1 in a multi-copy plasmid, as shown by Northern hybridization, gave a fourfold enhancement in total protein secretion. The repertoire of proteins that are found to be secreted in greater quantities include three known biologically active proteins: k1 killer toxin, alpha-factor, and acid phosphatase. The PSE-1 gene is located on chromosome XII of the yeast genome and codes for a hydrophobic protein containing 1089 amino acids. Haploid yeast cells that contained a LEU2 insertion mutation in PSE-1 grow very poorly, a phenotype similar to other conditional SEC mutants at restrictive temperature.
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Genetic Engineering Section, NRCC-BRI, Montreal, Quebec, Canada.
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Dignard
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Thomas
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D000135:Acid Phosphatase / Q000378:metabolism; D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D004271:DNA, Fungal; D004591:Electrophoresis, Polyacrylamide Gel; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D005800:Genes, Fungal; D000072235:Mating Factor; D026901:Membrane Transport Proteins; D008969:Molecular Sequence Data; D010455:Peptides / Q000378:metabolism; D010641:Phenotype; D010957:Plasmids; D018160:Receptors, Cytoplasmic and Nuclear / Q000235:genetics*; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins; D016415:Sequence Alignment
D004271:DNA, Fungal; D005656:Fungal Proteins; D026901:Membrane Transport Proteins; C483822:PSE1 protein, S cerevisiae; D010455:Peptides; D018160:Receptors, Cytoplasmic and Nuclear; D029701:Saccharomyces cerevisiae Proteins; D000072235:Mating Factor; D000135:Acid Phosphatase