Effects of several alcohols on glycosidase activities in rat liver and brain.
1. beta-D-Galactosidase, beta-D-glucuronidase and beta-NAc-D-glucosaminidase spec. acts from liver displayed statistically significant differences after treatments with certain alcohols. 2. No statistically significant difference was found for the kinetic parameters (Km and Vmax) of the indicated glycosidase activities. 3. beta-D-Glucuronidase and beta-NAc-D-glucosaminidase spec. acts from brain tissue showed statistically significant differences after treatments with certain alcohols. 4. beta-D-Glucuronidase and beta-NAc-D-glucosaminidase activities from liver showed a statistically significant decrease in the presence of in vitro ethanol. Tolerance effects were not observed.
San-Juan
I
I
Departamento de Bioquímica y Biología Molecular, Universidad de León, Spain.
García-Olalla
C
C
Chinchetru
M A
MA
Calvo
P
P
D000438:Alcohols / Q000494:pharmacology; D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000201:enzymology; D000431:Ethanol / Q000008:administration & dosage / Q000494:pharmacology*; D005260:Female; D006023:Glycoproteins / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000187:drug effects* / Q000378:metabolism; D007700:Kinetics; D008099:Liver / Q000187:drug effects* / Q000201:enzymology; D051381:Rats; D011919:Rats, Inbred Strains
D000438:Alcohols; D006023:Glycoproteins; D000431:Ethanol; D006026:Glycoside Hydrolases