Gram-scale enzymatic synthesis of a peptide bond.
The synthesis reaction of the peptide, N-Cbz-L-tryptophanyl-glycineamide, catalyzed by alpha-chymotrypsin was performed in a 20% water/80%, 1,4-butanediol mixture. The synthesis yield reached 90.9% at the end of the reaction and 72.3% after purification. The effects on the yield of both pH and the ratio between total initial concentrations of glycineamide and N-Cbz-L-tryptophan are examined. The high yield, specificity, simplicity and reproducibility of this method make it complementary of the chemical methods.
Deschrevel
B
B
Laboratoire Polymères, Biopolymères, Membranes, U.R.A. n. 500 C.N.R.S., Université de Rouen, Mont-Saint-Aignan.
Dugast
J Y
JY
Vincent
J C
JC
D002384:Catalysis; D002918:Chymotrypsin; D004798:Enzymes / Q000138:chemical synthesis; D008722:Methods; D010455:Peptides / Q000138:chemical synthesis*
D004798:Enzymes; D010455:Peptides; D002918:Chymotrypsin