title: Comparison of cyclosporine measurement in whole blood by high-performance liquid chromatography, monoclonal fluorescence polarization immunoassay, and monoclonal enzyme-multiplied immunoassay.
abstract: Monitoring of cyclosporine concentrations in whole blood is used routinely as a guide to adjusting dose so as to achieve optimal therapeutic benefit with minimal adverse effects. In the present study, we have compared a specific high-performance liquid chromatography (HPLC) assay with a fluorescence polarization immunoassay (TDx) and an enzyme-multiplied immunoassay (Emit). Both Emit and TDx assays employ a monoclonal antibody to cyclosporin A and therefore have the potential for a high degree of specificity. Blood specimens (EDTA as anticoagulant) were obtained from 113 patients (71 renal transplants, 17 liver transplants, and 25 other categories) taking cyclosporine and analysed by all three methods. There were significant correlations between results for HPLC and Emit (Emit = 10.54 + 1.07 x HPLC; r2 = 0.82, p less than 0.001) and between results for HPLC and TDx (TDx = 9.16 + 1.42 x HPLC; r2 = 0.82, p less than 0.001). Compared to HPLC analysis, 74% and 96%, respectively, of Emit and TDx results were to the left of the line of identity. The TDx monoclonal antibody appears to have a lesser degree of specificity than that used in the Emit assay. Mean concentrations of cyclosporine measured by Emit and TDx were 17% and 51% higher, respectively, than those measured by HPLC. Because of this overestimation, we suggest that both Emit and TDx methods may find their most appropriate use in routine therapeutic monitoring of renal transplant patients in whom metabolite concentrations are less variable over time.
authors.affiliation: Combined Unit in Clinical Pharmacology and Toxicology, University of Western Australia, Nedlands.
authors.forename: L J
authors.initials: LJ
authors.lastname: Dusci
authors.forename: L P
authors.initials: LP
authors.lastname: Hackett
authors.forename: G M
authors.initials: GM
authors.lastname: Chiswell
authors.forename: K F
authors.initials: KF
authors.lastname: Ilett
mesh_terms: D000911:Antibodies, Monoclonal; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D016572:Cyclosporine / Q000097:blood*; D005069:Evaluation Studies as Topic; D016231:Fluorescence Polarization Immunoassay / Q000379:methods; D006801:Humans; D007124:Immunoenzyme Techniques
chemical_list: D000911:Antibodies, Monoclonal; D016572:Cyclosporine