title: Molecular characterization of a Dirofilaria immitis cDNA encoding a highly immunoreactive antigen.
abstract: Dirofilaria immitis, a filarial nematode, is the causative agent of canine and feline heartworm disease. Previous research has demonstrated that immunity to D. immitis can be induced in dogs by repeated chemical abbreviation of infections while the parasite is a fourth-stage larva. Sera obtained from dogs immunized in this manner has been effective in passively transferring larval killing and stunting. These immune sera, by comparison to nonimmune sera from infected cohorts, recognize a number of unique D. immitis antigens, some of which are larval specific. In this study immune dog sera were used to screen a D. immitis larval cDNA expression library. Three overlapping cDNA clones, Di22, Di18 and Di16, were obtained that encode a portion of a large molecule, greater than 150 kDa, that is composed of multiples of a 399-bp repeat. This protein when immunoblotted with antibody against a recombinant expressed Di22 fusion protein is found in larval as well as adult extracts and excretory-secretory products, and is seen as a series of ascending subunits, each approximately 15 kDa larger than the previous one. This antigen is highly immunogenic, as evidenced by the strong reactivity of the recombinant expressed Di22 fusion protein with sera from immune dogs, microfilaremic dogs and infected amicrofilaremic dogs. While the function of this antigen is unknown it has significant sequence similarity with an allergen found in Ascaris.
authors.affiliation: Paravax, Inc., Mountain View, CA.
authors.forename: J
authors.initials: J
authors.lastname: Culpepper
authors.forename: R B
authors.initials: RB
authors.lastname: Grieve
authors.forename: L
authors.initials: L
authors.lastname: Friedman
authors.forename: M
authors.initials: M
authors.lastname: Mika-Grieve
authors.forename: G R
authors.initials: GR
authors.lastname: Frank
authors.forename: B
authors.initials: B
authors.lastname: Dale
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000947:Antigens, Helminth / Q000235:genetics* / Q000276:immunology; D001483:Base Sequence; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D004183:Dirofilaria immitis / Q000235:genetics* / Q000276:immunology; D004285:Dogs; D015801:Helminth Proteins / Q000276:immunology; D008969:Molecular Sequence Data; D012689:Sequence Homology, Nucleic Acid
chemical_list: D000947:Antigens, Helminth; D015801:Helminth Proteins; D004247:DNA