Bronchial-associated lymphoid tissue (BALT) response to airway challenge with cigarette smoke, bovine antigen and anti-pulmonary serum.
The bronchial-associated lymphoid tissue (BALT) is a lymphoepithelial organ, related to the immune defence of the lung and to alveolar clearance, which changes size in certain states of disease. Changes in the size of BALT were quantified and compared, and Spearman's test was used to test the relation with the bronchial epithelium. A total of 180 rats were used, divided into 6 groups of 30 as follows: 1) untreated controls; 2) exposed to cigarette smoke for two months; 3) treated with anti-pulmonary serum three doses daily over five days; 4) exposed to cigarette smoke and treated with anti-pulmonary serum; 5) sensitized with bovine albumin and exposed to an environment containing this antigen for two months; 6) exposed to cigarette smoke and bovine albumin. The lungs were processed for histological study, and were stained with the PAS-Alcian blue method. The main left bronchi BALT was studied, and the following were quantified: Lymphatic area (LA), as a percentage of the lung surface occupied by BALT; the flat epithelium (FEp), as the length of bronchial epithelium anatomically related to LA, whose cells tend to adopt a flat shape; the Contact epithelium (Cep), as the length of bronchial epithelium which is in direct contact with the LA. A percentage count of bronchial cells was made in the following classifications: globet cells; globet cells stained with the PAS-Alcian blue method; flat cells; lymphoepithelium cells; columnar cells; and bronchial epithelium cells excluding the above two cell types.(ABSTRACT TRUNCATED AT 250 WORDS)
Escolar Castellón
J D
JD
Department of Morphological Science, Faculty of Medicine, Hospital Provincial, Zaragoza, Spain.
Escolar Castellón
A
A
Roche Roche
P A
PA
Miñana Amada
C
C
D000418:Albumins / Q000276:immunology; D000818:Animals; D000906:Antibodies / Q000008:administration & dosage; D000941:Antigens / Q000008:administration & dosage; D001980:Bronchi / Q000276:immunology / Q000473:pathology*; D004848:Epithelium / Q000473:pathology; D005260:Female; D008221:Lymphoid Tissue / Q000276:immunology / Q000473:pathology*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012907:Smoking / Q000473:pathology*
D000418:Albumins; D000906:Antibodies; D000941:Antigens