Isolation of FMRFa-like peptides from the DB-containing connective tissue of Helix aspersa.
The endocrine dorsal bodies (DB) of Helix aspersa are innervated by axons from the central nervous system, which establish synapse-like structures (SLS) with the DB cells. Previous immunocytochemical studies suggested the presence of FMRFa-like substances in nerves of the DB area and in SLS. This paper reports on biochemical attempts undertaken in order to investigate the nature of these substances: the use of HPLC and RIA confirms the presence of three FMRFa-like peptides in the DB-containing connective tissue among which one is probably the FMRFa itself.
Mounzih
K
K
Laboratoire de Zoologie-Embryologie, UA CNRS 687, Faculté des Sciences et des Techniques, Besançon, France.
Griffond
B
B
D000595:Amino Acid Sequence; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003238:Connective Tissue / Q000737:chemistry*; D004702:Endocrine Glands / Q000737:chemistry*; D019835:FMRFamide; D005724:Ganglia / Q000378:metabolism; D006372:Helix, Snails / Q000378:metabolism*; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000737:chemistry / Q000302:isolation & purification*; D011863:Radioimmunoassay
D009479:Neuropeptides; D019835:FMRFamide