Measurement of pH to quantify urease activity.
The rate of change of pH caused by the hydrolysis of urea was measured for urease solutions of 18 different concentrations. Concentrations were converted into an activity, A (measured in IU/cm3), by using a titrimetric method to assay the urease sample. For activities in the range 0.6-38 IU/cm3, A was related to the initial rate of change of pH, (dpH/dt)0, (measured in s-1) by the empirical relationship: A = 549(dpH/dt)0-1423(dpH/dt)2(0). Values of (dpH/dt)0 were sensitive to changes in the urease activity of about 0.6 IU/cm3.
Bibby
J M
JM
Department of Medical Biophysics, University of Manchester, UK.
Hukins
D W
DW
D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007700:Kinetics; D010940:Plant Proteins / Q000378:metabolism*; D014508:Urea / Q000378:metabolism; D014510:Urease / Q000378:metabolism*
D010940:Plant Proteins; D014508:Urea; D014510:Urease