title: Photodynamic inactivation of retroviruses by phthalocyanines: the effects of sulphonation, metal ligand and fluoride.
abstract: The photodynamic inactivation of retroviruses was investigated using aluminium and zinc phthalocyanine (Pc) derivatives. The N2 retrovirus packaged in either of the two murine cell lines, Psi2 and PA317, was used as a model for enveloped viruses. AlPc derivatives were found to be more effective photodynamically for inactivation of the viruses than the corresponding ZnPc derivatives. Sulphonation of the Pc macrocycle reduced its photodynamic activity progressively for both AlPc and ZnPc. Fluoride at 5 mM during light exposure completely protected viruses against inactivation by AlPc. In the presence of F-, inactivation by the sulphonated derivatives AlPcS1 and AlPcS4 was reduced 2.5- and twofold respectively. In a biological membrane (erythrocyte ghosts), F- had no significant effect on AlPcS4-sensitized lipid peroxidation. Under similar conditions, cross-linking of spectrin monomers in ghosts is drastically inhibited (E. Ben-Hur and A. Orenstein, Int. J. Radiat. Biol., 60 (1991) 293-301). Since Pc derivatives do not inactivate non-enveloped viruses, it is hypothesized that inactivation occurs by photodynamic damage to envelope protein(s). Substitution of sulphonic acid residues reduces the binding of Pc derivatives to the envelope protein(s), thereby diminishing their photodynamic efficacy and the ability of F- to modify it.
authors.affiliation: Sylvius Laboratory, Department of Medical Biochemistry, Leiden, Netherlands.
authors.forename: E
authors.initials: E
authors.lastname: Ben-Hur
authors.forename: R C
authors.initials: RC
authors.lastname: Hoeben
authors.forename: H
authors.initials: H
authors.lastname: Van Ormondt
authors.forename: T M
authors.initials: TM
authors.lastname: Dubbelman
authors.forename: J
authors.initials: J
authors.lastname: Van Steveninck
mesh_terms: D000535:Aluminum / Q000494:pharmacology*; D000818:Animals; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D004307:Dose-Response Relationship, Radiation; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D006801:Humans; D007211:Indoles / Q000494:pharmacology*; D054833:Isoindoles; D008027:Light; D015227:Lipid Peroxidation / Q000187:drug effects; D051379:Mice; D009942:Organometallic Compounds / Q000494:pharmacology*; D012190:Retroviridae / Q000187:drug effects* / Q000528:radiation effects; D015032:Zinc / Q000494:pharmacology*; D017967:Zinc Compounds
chemical_list: D007211:Indoles; D054833:Isoindoles; D009942:Organometallic Compounds; D017967:Zinc Compounds; C052159:Zn(II)-phthalocyanine; C060570:aluminum phthalocyanine; D000535:Aluminum; D015032:Zinc