Rate laws for the exchange of guanine nucleotides bound to EF-Tu catalysed by EF-Ts.
The rate laws governing the exchange of guanine nucleotides bound to EF-Tu catalysed by EF-Ts are set out and the information that is available from various studies is analysed in terms of the laws. In particular the consequences of k-1 being equal to k-4 in the reaction sequence as set out below are examined in relation to results obtained by Hwang and Miller (J. Biol. Chem. 260, 11498-11502) and the contradiction these pose in relation to other published results. Measurement of on and off rate constants for binding of GTP to EF-Tu.EF-Ts and of the affinity of EF-Ts for EF-Tu.GTP should prove of importance in resolving discrepancies.
Manchester
K L
KL
Department of Biochemistry, University of the Witwatersrand, Johannesburg, South Africa.
D001665:Binding Sites; D002384:Catalysis; D006150:Guanine Nucleotides / Q000378:metabolism*; D006153:Guanosine Diphosphate / Q000378:metabolism; D006160:Guanosine Triphosphate / Q000378:metabolism; D007700:Kinetics; D008956:Models, Chemical; D010444:Peptide Elongation Factor Tu / Q000378:metabolism*; D010445:Peptide Elongation Factors / Q000378:metabolism*
D006150:Guanine Nucleotides; D010445:Peptide Elongation Factors; C023512:elongation factor Ts; D006153:Guanosine Diphosphate; D006160:Guanosine Triphosphate; D010444:Peptide Elongation Factor Tu