title: Dose dependence of acetylcholinesterase activity in neuroblastoma cells exposed to modulated radio-frequency electromagnetic radiation.
abstract: Radio-frequency electromagnetic radiation (RFR) at 915 and 147 MHz, when sinusoidally amplitude modulated (AM) at 16 Hz, has been shown to enhance release of calcium ions from neuroblastoma cells in culture. The dose-response relation is unusual, consisting of two power-density "windows" in which enhanced efflux occurs, separated by power-density regions in which no effect is observed. To explore the physiological importance of these findings, we have examined the impact of RFR exposure on a membrane-bound enzyme, acetylcholinesterase (AChE), which is intimately involved with the acetylcholine (ACh) neurotransmitter system. Neuroblastoma cells (NG108), exposed for 30 min to 147-MHz radiation, AM at 16 Hz, demonstrated enhanced AChE activity, as assayed by a procedure using 14C-labeled ACh. Enhanced activity was observed within a time window between 7.0 and 7.5 h after the cells were plated and only when the exposure occurred at power densities identified in a previous report as being effective for altering the release of calcium ions. Thus RFR affects both calcium-ion release and AChE activity in nervous system-derived cells in culture in a common dose-dependent manner.
authors.affiliation: Department of Botany, Howard University, Washington, DC 20059.
authors.forename: S K
authors.initials: SK
authors.lastname: Dutta
authors.forename: K
authors.initials: K
authors.lastname: Das
authors.forename: B
authors.initials: B
authors.lastname: Ghosh
authors.forename: C F
authors.initials: CF
authors.lastname: Blackman
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D004307:Dose-Response Relationship, Radiation; D051379:Mice; D009447:Neuroblastoma / Q000201:enzymology* / Q000473:pathology; D011846:Radio Waves; D051381:Rats; D014407:Tumor Cells, Cultured
chemical_list: D000110:Acetylcholinesterase