title: Cleavage of type VIII collagen by human neutrophil elastase.
abstract: In this report, the susceptibility of type VIII collagen to human neutrophil elastase is compared to other extracellular matrix components. Type X collagen is degraded to specific fragments at a substrate to enzyme ratio of 5:1 after 20 h at room temperature, but type VIII collagen is almost completely degraded after only 4 h incubation at a substrate to enzyme ratio of 50:1 and partly degraded after only 15 min. Laminin, merosin and types I, III, IV and V collagen exhibit no susceptibility to neutrophil elastase under the latter conditions, while fibronectin is degraded.
authors.affiliation: Wallaceville Animal Research Centre, Upper Hutt, New Zealand.
authors.forename: R
authors.initials: R
authors.lastname: Kittelberger
authors.forename: T J
authors.initials: TJ
authors.lastname: Neale
authors.forename: K T
authors.initials: KT
authors.lastname: Francky
authors.forename: N S
authors.initials: NS
authors.lastname: Greenhill
authors.forename: G J
authors.initials: GJ
authors.lastname: Gibson
mesh_terms: D003094:Collagen / Q000737:chemistry / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D005353:Fibronectins / Q000378:metabolism; D006801:Humans; D007797:Laminin / Q000378:metabolism; D019272:Leukocyte Elastase; D008565:Membrane Proteins / Q000378:metabolism; D010196:Pancreatic Elastase / Q000378:metabolism*; D013379:Substrate Specificity; D013696:Temperature
chemical_list: D005353:Fibronectins; D007797:Laminin; D008565:Membrane Proteins; D003094:Collagen; D010196:Pancreatic Elastase; D019272:Leukocyte Elastase