title: An artificial blood vessel with an endothelial-cell monolayer.
abstract: An artificial blood vessel with an endothelial-cell monolayer was used as an arterial substitute in rats. Endothelial cells were isolated from the aorta of a Wistar rat by the digestion method. The cell identification was established by the cobblestone appearance of a confluent cell monolayer, by an expression of factor VIII-related antigen, and by the presence of Weibel-Palade bodies. The luminal surface of the thin-walled polytetrafluoroethylene (PTFE) graft (4 mm in diameter and 10 mm in length) was coated with an endothelial-cell monolayer for 7 days in vitro. An interpositional graft was placed using the endothelial cell-coated PTFE prosthesis on the right common carotid artery in seven rats. A total of 10 rats received an interpositional graft with the noncoated PTFE prosthesis as a control. The patency rate at 1 month after implantation was significantly higher in the coated group than in the control group. The vascular prosthesis with an endothelial-cell monolayer is a promising technique to inhibit the development of thrombosis.
authors.affiliation: Department of Neurosurgery, Fukui Medical School, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Kobayashi
authors.forename: M
authors.initials: M
authors.lastname: Kabuto
authors.forename: H
authors.initials: H
authors.lastname: Ide
authors.forename: K
authors.initials: K
authors.lastname: Hosotani
authors.forename: T
authors.initials: T
authors.lastname: Kubota
mesh_terms: D000818:Animals; D001011:Aorta / Q000166:cytology; D001807:Blood Vessel Prosthesis / Q000379:methods*; D002339:Carotid Arteries / Q000601:surgery; D002478:Cells, Cultured; D016009:Chi-Square Distribution; D004730:Endothelium, Vascular / Q000166:cytology*; D008297:Male; D011138:Polytetrafluoroethylene; D051381:Rats; D011919:Rats, Inbred Strains; D014654:Vascular Patency
chemical_list: D011138:Polytetrafluoroethylene