Influenza virus A infection induces interleukin-8 gene expression in human airway epithelial cells.
To determine the role of the airway epithelial cell in mediating virus-induced inflammation, we infected primary cultures of human airway epithelial cells with human influenza type A/Port Chalmers/72 (H3N2). After two days, the medium was collected for measurement of the chemotactic cytokine interleukin-8 by enzyme-linked immunosorbent assay. The RNA was extracted from the cells for analysis of interleukin-8 mRNA by Northern blot analysis. Interleukin-8 production was more than doubled by viral infection, while interleukin-8 mRNA was increased four-fold. Thus induction of interleukin-8 gene expression in virus-infected airway epithelium may be an important early step leading to virus-induced airway inflammation.
Choi
A M
AM
Division of Pulmonary and Critical Care Medicine, Johns Hopkins Asthma and Allergy Center, Baltimore, MD.
Jacoby
D B
DB
D001483:Base Sequence; D002478:Cells, Cultured; D004247:DNA; D004847:Epithelial Cells; D004848:Epithelium / Q000378:metabolism / Q000382:microbiology; D005786:Gene Expression Regulation; D006801:Humans; D009980:Influenza A virus / Q000502:physiology*; D016209:Interleukin-8 / Q000235:genetics* / Q000378:metabolism; D008168:Lung / Q000166:cytology / Q000378:metabolism / Q000382:microbiology*; D008969:Molecular Sequence Data; D014779:Virus Replication
D016209:Interleukin-8; D004247:DNA