title: Carbohydrates in mammalian tryptophanyl-tRNA synthetase.
abstract: Homogeneous preparations of bovine tryptophanyl-tRNA synthetase (EC 6.1.1.2) contain monosaccharides (mannose, fucose, galactose, N-acetylglucosamine) as revealed by liquid chromatography. Their content comprises 2.5-3.0% (w/w) of the enzyme composed of two subunits (60 kDa x 2). The same set of sugars was detected in elastase and CNBr-generated fragments (with molecular masses of approx. 40 kDa and 30 kDa, respectively). It is concluded that bovine tryptophanyl-tRNA synthetase, in addition to being a metallo- and phosphoprotein, is also a glycoprotein.
authors.affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow.
authors.forename: G K
authors.initials: GK
authors.lastname: Kovaleva
authors.forename: A O
authors.initials: AO
authors.lastname: Zheltova
authors.forename: T V
authors.initials: TV
authors.lastname: Nikitushkina
authors.forename: T A
authors.initials: TA
authors.lastname: Egorov
authors.forename: A Ch
authors.initials: ACh
authors.lastname: Musoljamov
authors.forename: L L
authors.initials: LL
authors.lastname: Kisselev
mesh_terms: D000818:Animals; D050260:Carbohydrate Metabolism; D002417:Cattle; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D014369:Tryptophan-tRNA Ligase / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D014369:Tryptophan-tRNA Ligase