A factor derived from chick embryo retina which inhibits DNA synthesis of retina itself.
Chick embryo retinas contain a peptide factor that inhibits DNA synthesis in explants of chick embryo retina. The inhibitory factor, obtained by acid/ethanol extraction from 15-day-old chick embryo retinas, was partially purified by affinity chromatography on heparin-sepharose CL-6B and gel filtration on Sephadex G-100. The inhibitor reduced DNA synthesis with maximal effects observed in retinal explants from 7 to 8-day-old chick embryos. The inhibitory effect became apparent after 10 h of incubation and reached the maximum levels after 16 h. DNA-inhibiting activity was heat and acid-stable and was destroyed by trypsin and alkaline treatments. The inhibitory effect was observed in retinal explants incubated in a medium free from L-glutamine, and the addition of this compound to the medium reduced the inhibitory effect in a concentration-dependent manner.
Calvaruso
G
G
Institute of Biological Chemistry, University of Palermo, Policlinico, Italy.
Vento
R
R
Taibi
G
G
Giuliano
M
M
Tesoriere
G
G
D000818:Animals; D001685:Biological Factors / Q000302:isolation & purification / Q000502:physiology*; D002455:Cell Division / Q000502:physiology; D002642:Chick Embryo; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D046508:Culture Techniques; D004247:DNA / Q000096:biosynthesis*; D012160:Retina / Q000196:embryology / Q000378:metabolism*; D013936:Thymidine / Q000378:metabolism; D014020:Tissue Extracts / Q000032:analysis
D001685:Biological Factors; D014020:Tissue Extracts; D004247:DNA; D013936:Thymidine