Expression of the brain-type glucose transporter is restricted to brain and neuronal cells in mice.
Northern blot analysis of human tissues has demonstrated the expression of the brain-type glucose transporter isoform (GLUT 3) in liver, muscle and fat, raising the possibility that this transporter isoform may play a role in the regulation of glucose disposal in these tissues in response to insulin. We have raised an anti-peptide antibody against the C-terminal 13 amino acids of the murine homologue of this transporter isoform, and determined its tissue distribution in mouse tissues and murine-derived cell lines. The antibodies recognise a glycoprotein of about 50 kilodaltons, expressed at high levels in murine brain. In contrast to human tissues, the expression of GLUT 3 in mice is restricted to the brain, and no immunoreactivity was observed in either liver, fat or muscle membranes, or in murine 3T3-L1 fibroblasts or adipocytes. In contrast, high levels of expression of this isoform were observed in the NG 108 neuroblastoma x glioma cell line, a hybrid cell derived from rat glioma and mouse neuroblastoma cells. Taken together, these data suggest that the expression of GLUT 3 in rodents is restricted to non-insulin responsive neuronal cells and hence it is likely that the factors regulating the expression of this transporter in rodents differ to those in humans.
Gould
G W
GW
Department of Biochemistry, University of Glasgow, Scotland, United Kingdom.
Brant
A M
AM
Kahn
B B
BB
Shepherd
P R
PR
McCoid
S C
SC
Gibbs
E M
EM
D016475:3T3 Cells; D000273:Adipose Tissue / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000502:physiology*; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D005910:Glioma; D006801:Humans; D006822:Hybrid Cells; D007106:Immune Sera; D051379:Mice; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009004:Monosaccharide Transport Proteins / Q000032:analysis / Q000235:genetics*; D009447:Neuroblastoma; D009474:Neurons / Q000378:metabolism / Q000502:physiology*; D009928:Organ Specificity; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology; D051381:Rats
D007106:Immune Sera; D009004:Monosaccharide Transport Proteins; D010455:Peptides