title: Gonadotropin surge increases fluorescent-tagged low-density lipoprotein uptake by macaque granulosa cells from preovulatory follicles.
abstract: In the primate ovary, luteal steroidogenesis is largely dependent upon cholesterol derived from receptor-mediated uptake of circulating low-density lipoprotein (LDL). However, granulosa cells (GC) of preovulatory follicles possess few LDL binding sites compared to those present in developing and mature corpora lutea. We recently reported (Endocrinology 1991; 129:3247-3253) that uptake of LDL tagged with the fluorescent probe 1,1'-dioctadecyl-3,3,3',3'-tetramethyl-indocarbocyanine perchlorate (DiI) can be monitored in macaque luteal cells by fluorescence-activated flow cytometry. This study was designed to determine whether an ovulatory stimulus induced uptake of DiI-LDL in GC aspirated from preovulatory follicles of macaques undergoing ovarian stimulation. Development of multiple large follicles was stimulated in adult rhesus macaques with human gonadotropin treatment for 9 days. On Day 10, monkeys received either no ovulatory stimulus or 1000 IU hCG to initiate ovulatory events. GC were aspirated on Day 10 in monkeys receiving no ovulatory stimulus (nonluteinized GC) or 27 h or 34 h after hCG injection (luteinizing GC). GC were resuspended in Ham's F-10 medium + 0.1% BSA and incubated with several concentrations (0-25 micrograms/ml) of DiI-LDL (Biomedical Technologies, Stoughton, MA) for various time intervals (2-60 min). DiI-LDL uptake by GC was time- and concentration-dependent. Coincubation of cells with DiI-LDL and unlabeled LDL dose-dependently suppressed the percentage of fluorescent cells. In contrast, coincubation with up to a 250-fold excess of acetylated LDL or high-density lipoprotein did not alter the percentage of fluorescent GC.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Division of Reproductive Biology and Behavior, Oregon Regional Primate Research Center, Beaverton 97006.
authors.forename: J D
authors.initials: JD
authors.lastname: Brannian
authors.forename: S M
authors.initials: SM
authors.lastname: Shiigi
authors.forename: R L
authors.initials: RL
authors.lastname: Stouffer
mesh_terms: D000818:Animals; D002232:Carbocyanines; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D004958:Estradiol / Q000097:blood; D005260:Female; D005434:Flow Cytometry; D005456:Fluorescent Dyes; D006107:Granulosa Cells / Q000187:drug effects / Q000378:metabolism*; D008077:Lipoproteins, LDL / Q000378:metabolism*; D007986:Luteinizing Hormone / Q000378:metabolism*; D008253:Macaca mulatta; D010060:Ovulation / Q000502:physiology*; D011374:Progesterone / Q000097:blood
chemical_list: D002232:Carbocyanines; D006063:Chorionic Gonadotropin; D005456:Fluorescent Dyes; D008077:Lipoproteins, LDL; C024286:3,3'-dioctadecylindocarbocyanine; D011374:Progesterone; D004958:Estradiol; D007986:Luteinizing Hormone