title: Immunohistochemical localization of fibronectin as a tool for the age determination of human skin wounds.
abstract: We analyzed the distribution of fibronectin in routinely embedded tissue specimens from 53 skin wounds and 6 postmortem wounds. In postmortem wounds a faint but focal positive staining was exclusively found at the margin of the specimens which did not extend into the adjacent stroma. Vital wounds were classified into 3 groups. The first comprising lesions with wound ages ranging from a few seconds to 30 min, the second comprising those with wound ages up to 3 weeks, and the third group with lesions more than 3 weeks old. Ten out of 17 lesions with a wound age up to 30 min showed a clear positive reaction within the wound area. Three specimens in this group were completely negative, while in 4 additional cases the result was not significantly different from postmortem lesions. These 7 cases were characterized by acute death with extremely short survival times (only seconds). In wounds up to 3 weeks old fibronectin formed a distinct network containing an increasing number of inflammatory cells corresponding to the wound age. In 2 cases with a survival time of 17 days and in all wounds older than 3 weeks fibronectin was restricted to the surface of fibroblasts and to parallel arranged fibers in the granulation tissue without any network structures. We present evidence that fibronectin is a useful marker for vital wounds with a survival time of more than a few minutes. Fibronectin appears before neutrophilic granulocytes migrate into the wound area.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Legal Medicine, University of Munich, Federal Republic of Germany.
authors.forename: P
authors.initials: P
authors.lastname: Betz
authors.forename: A
authors.initials: A
authors.lastname: Nerlich
authors.forename: J
authors.initials: J
authors.lastname: Wilske
authors.forename: J
authors.initials: J
authors.lastname: Tübel
authors.forename: I
authors.initials: I
authors.lastname: Wiest
authors.forename: R
authors.initials: R
authors.lastname: Penning
authors.forename: W
authors.initials: W
authors.lastname: Eisenmenger
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D005353:Fibronectins / Q000032:analysis*; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D008875:Middle Aged; D011180:Postmortem Changes; D012867:Skin / Q000293:injuries*; D014945:Wound Healing / Q000502:physiology; D014947:Wounds and Injuries / Q000473:pathology*
chemical_list: D005353:Fibronectins