Application of highly polymorphic DNA markers to the identification of HeLa cell sublines.
Polymorphic DNA markers were used to identify eight sublines derived from HeLa. Using five highly polymorphic minisatellite DNA probes, these cell lines were distinguished and classified into six groups by Southern blot analysis. Polymorphic DNA markers, therefore, can provide a useful tool for monitoring genetic changes of a cell line during culture and for distinguishing sublines derived from the same origin.
Kataoka
E
E
Division of Genetics and Mutagenesis, National Institute of Hygienic Sciences, Tokyo, Japan.
Honma
M
M
Ohnishi
K
K
Sofuni
T
T
Mizusawa
H
H
D015139:Blotting, Southern; D004276:DNA, Satellite / Q000032:analysis*; D005819:Genetic Markers; D006367:HeLa Cells / Q000737:chemistry*; D006801:Humans; D010641:Phenotype
D004276:DNA, Satellite; D005819:Genetic Markers