On-line dialysis, liquid chromatography and post-column reaction detection of oxytetracycline in salmon muscle extracts.
The development of a sensitive automated method for residue control of oxytetracycline (OTC) in salmon muscle is described. Tissue homogenate is dialysed and the dialysate enriched on a small on-line polystyrene column. OTC and the internal standard (tetracycline) are separated by HPLC on a polystyrene column using an ion-pair eluent system. The column effluent is mixed with sodium hydroxide and irradiated at 366 nm and the resulting derivatives monitored by means of a fluorescence detector (excitation: 358 nm, emission: 460 nm). By the method OTC is detected down to 5 ng g-1. The standard curve was linear (r = 0.9999) over the range 50-1000 ng g-1. Within-day and between-day relative standard deviations (n = 6) at 50 and 200 ng g-1 ranged from 1.0 to 1.7%.
Agasøster
T
T
Institute of Pharmacy, University of Oslo, Norway.
Rasmussen
K E
KE
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003956:Dialysis; D009132:Muscles / Q000737:chemistry*; D010118:Oxytetracycline / Q000032:analysis*; D012474:Salmon / Q000378:metabolism*
D010118:Oxytetracycline