Protein B23 (M.W./pI = 37 kD/5.1) is the only major protein extracted from HeLa nucleoli with 3M urea.
HeLa nucleoli were isolated using the NP-40 method and subsequently extracted with 3M urea. The extract was incubated at 60 degrees C for 30 min, and precipitated proteins were removed by centrifugation. The supernatant was analyzed by one- and two-dimensional SDS polyacrylamide gel electrophoresis (PAGE). Protein B23 was the only major protein extracted from HeLa nucleoli by this procedure. Using this procedure, 1 mg of protein B23 was obtained from 2 g of HeLa cells. The purity of the extracted protein B23 was 98%, as measured by one- and two-dimensional gel electrophoresis.
Yung
B Y
BY
Department of Pharmacology, Chang Gung Medical College, Taiwan, R.O.C.
Hui
E K
EK
Chan
P K
PK
D002466:Cell Nucleolus / Q000737:chemistry*; D002498:Centrifugation; D015180:Electrophoresis, Gel, Two-Dimensional; D006367:HeLa Cells / Q000737:chemistry; D006801:Humans; D007526:Isoelectric Point; D008970:Molecular Weight; D009687:Nuclear Proteins / Q000302:isolation & purification*; D000090243:Nucleophosmin; D010750:Phosphoproteins / Q000302:isolation & purification; D014508:Urea
D009687:Nuclear Proteins; D010750:Phosphoproteins; D000090243:Nucleophosmin; D014508:Urea