title: SV2, a brain synaptic vesicle protein homologous to bacterial transporters.
abstract: Synaptic vesicle protein 2 (SV2) is a membrane glycoprotein specifically localized to secretory vesicles in neurons and endocrine cells. As a first step toward understanding the function of SV2 in neural secretion, a rat brain complementary DNA (cDNA) that encodes SV2 was isolated and characterized. Analyses of this cDNA predict that SV2 contains 12 transmembrane domains. The NH2-terminal half of the protein shows significant amino acid sequence identity to a family of bacterial proteins that transport sugars, citrate, and drugs. Expression of the SV2 cDNA in COS cells yielded a high level of SV2-like immunoreactivity distributed in a reticular and punctate pattern, which suggests localization to intracellular membranes. Its localization to vesicles, predicted membrane topology, and sequence identity to known transporters suggest that SV2 is a synaptic vesicle-specific transporter.
authors.affiliation: Howard Hughes Medical Institute, Stanford University, CA 94305.
authors.forename: S M
authors.initials: SM
authors.lastname: Bajjalieh
authors.forename: K
authors.initials: K
authors.lastname: Peterson
authors.forename: R
authors.initials: R
authors.lastname: Shinghal
authors.forename: R H
authors.initials: RH
authors.lastname: Scheller
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics; D001921:Brain / Q000378:metabolism*; D002352:Carrier Proteins / Q000235:genetics; D004247:DNA / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D005656:Fungal Proteins / Q000235:genetics; D008562:Membrane Glycoproteins / Q000235:genetics*; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics*; D016133:Polymerase Chain Reaction; D051381:Rats; D012689:Sequence Homology, Nucleic Acid; D014162:Transfection
chemical_list: D001426:Bacterial Proteins; D002352:Carrier Proteins; D005656:Fungal Proteins; D008562:Membrane Glycoproteins; D009419:Nerve Tissue Proteins; C489618:Sv2a protein, rat; D004247:DNA