Effect of DL-propranolol on nitric oxide production in perfused rat hindquarters.
The effect of DL-propranolol on NO release in perfused rat hindquarters was studied by using oxyhemoglobin as a capture system to allow the quantitative assay of NO production. In some experiments the stable prostacyclin metabolite 6-keto-PGF1 alpha (6-keto) was simultaneously assayed. We observed that: (1) DL-propranolol induced an increase in NO and 6-keto release. The dextro isomer was inactive; (2) DL-propranolol-induced NO release was only slightly reduced by acetylsalicylic acid in a concentration that inhibits prostacyclin synthesis, and was abolished by the chemical removal of the endothelium with 3-3 cholamidopropyl dimethylammonium 1-propane sulphonate (CHAPS) applied before or during stimulation; (3) NG-nitro-L-arginine blocked DL-propranolol-induced NO production, an effect that was antagonized by L-arginine but not by its dextro isomer.
Mota-Filipe
H
H
Laboratório de Farmacologia, Faculdade de Farmácia de Lisboa, Portugal.
Castro
M
M
Gião-T-Rico
J M
JM
D015121:6-Ketoprostaglandin F1 alpha / Q000378:metabolism; D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives / Q000494:pharmacology; D001241:Aspirin / Q000494:pharmacology; D001285:Atropine / Q000494:pharmacology; D002217:Carbachol / Q000494:pharmacology; D002793:Cholic Acids / Q000494:pharmacology; D004730:Endothelium, Vascular / Q000502:physiology*; D011464:Epoprostenol / Q000378:metabolism; D008297:Male; D009569:Nitric Oxide / Q000378:metabolism*; D019335:Nitroarginine; D010108:Oxyhemoglobins / Q000378:metabolism; D011433:Propranolol / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains
D002793:Cholic Acids; D010108:Oxyhemoglobins; D019335:Nitroarginine; D009569:Nitric Oxide; D015121:6-Ketoprostaglandin F1 alpha; D001285:Atropine; D002217:Carbachol; D001120:Arginine; D011433:Propranolol; D011464:Epoprostenol; C028213:3-((3-cholamidopropyl)dimethylammonium)-1-propanesulfonate; D001241:Aspirin