Novel quantitative methods for the determination of biomaterial cytotoxicity.
Two novel methods for the determination of biomaterial cytotoxicity using cell culture are presented. The methods combine a standardized protocol for producing extracts from medical devices with either the established MTT assay or a new fluorimetric assay. The suitability of both methods for evaluating the toxicity of candidate materials was demonstrated by resolution of the differences in the toxic effects of serial dilutions of a PVC extract on BHK21 and HT1080 cells. The tests yield highly reproducible, quantitative results and can be applied to materials in the usual physical forms applicable to artificial organs.
Smith
M D
MD
Wolfson Centre, University of Strathclyde, Glasgow, U.K.
Barbenel
J C
JC
Courtney
J M
JM
Grant
M H
MH
D000818:Animals; D001672:Biocompatible Materials / Q000633:toxicity*; D002460:Cell Line; D002478:Cells, Cultured / Q000187:drug effects; D003124:Colorimetry / Q000379:methods; D004396:Coloring Agents; D006224:Cricetinae; D005470:Fluorometry / Q000379:methods; D007202:Indicators and Reagents; D008422:Materials Testing / Q000379:methods*; D011095:Polyethylenes / Q000633:toxicity; D011143:Polyvinyl Chloride / Q000633:toxicity; D013778:Tetrazolium Salts; D013844:Thiazoles
D001672:Biocompatible Materials; D004396:Coloring Agents; D007202:Indicators and Reagents; D011095:Polyethylenes; D013778:Tetrazolium Salts; D013844:Thiazoles; D011143:Polyvinyl Chloride; C022616:thiazolyl blue