title: A cleavable biotinylating agent and its use in protein electroblots.
abstract: A direct method for the synthesis of N-biotinyl penicillamine is described. It has been shown to be a convenient biotinylating agent for antibodies which have been previously coupled with SPDP. The biotinylated antibodies can be used to detect antigens on protein electroblots using 125I-labelled streptavidin and radioautography. The biotin and its attached streptavidin and radiolabel can be removed under mild conditions and the blot reprobed with a different antibody using an identical protocol.
authors.affiliation: Molecular Immunopathology Unit, M.R.C. Centre, Cambridge, UK.
authors.forename: M J
authors.initials: MJ
authors.lastname: Hobart
authors.forename: B A
authors.initials: BA
authors.lastname: Fernie
authors.forename: P J
authors.initials: PJ
authors.lastname: Lachmann
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology; D001345:Autoradiography; D001710:Biotin; D010396:Penicillamine; D011506:Proteins / Q000032:analysis*
chemical_list: D000911:Antibodies, Monoclonal; D011506:Proteins; D001710:Biotin; D010396:Penicillamine