title: Hormonal regulation of the type III isoform of C-kinase in porcine ovarian tissues.
abstract: C-kinase activity is notably increased in corpora lutea (CL) compared to preovulatory follicles of porcine ovaries. Our purpose was to identify the C-kinase isoform(s) involved in this increase and to examine the expression of C-kinase in ovarian tissues at different stages. The major component of C-kinase activity in the CL was isoform III, with a molecular weight (M(r)) of 80,000. Minor activities were attributed to the type II isoform (M(r) = 80,000) and an unidentified C-kinase activity (M(r) = 77,000). C-kinase was not partitioned differently in preovulatory follicles and CL as demonstrated by tissue homogenization in the presence of a detergent and increased chelators. A 3-fold increase in immunoreactive C-kinase was detected in postovulatory follicles relative to preovulatory. A second, nearly 3-fold increase in C-kinase was detected in mature CL relative to postovulatory follicles. These increases are examined in the context of the complex hormonal regulation of the porcine CL.
authors.affiliation: Department of Cell, Molecular and Structural Biology, Northwestern University Medical School, Chicago 60611.
authors.forename: D A
authors.initials: DA
authors.lastname: DeManno
authors.forename: E T
authors.initials: ET
authors.lastname: Maizels
authors.forename: M
authors.initials: M
authors.lastname: Hunzicker-Dunn
mesh_terms: D000818:Animals; D002118:Calcium / Q000502:physiology; D003338:Corpus Luteum / Q000201:enzymology; D005260:Female; D015151:Immunoblotting; D007527:Isoenzymes / Q000378:metabolism; D006080:Ovarian Follicle / Q000201:enzymology; D010053:Ovary / Q000201:enzymology*; D011493:Protein Kinase C / Q000302:isolation & purification / Q000378:metabolism*; D013552:Swine
chemical_list: D007527:Isoenzymes; D011493:Protein Kinase C; D002118:Calcium