title: Effects of AFB1 embryonic exposure on chicken mononuclear phagocytic cell functions.
abstract: Effects of embryonic exposure to aflatoxin-B1 (AFB1) on the postnatal development of chicken mononuclear phagocytic system function was examined. Single exposure of 6-d chick embryos to 0.1, 0.5, and 1 micrograms AFB1 in 10 microL acetone was employed. Control embryos received 10 microliters solvent and sham-treated controls included embryos with a hole in the egg shell with no compound added. Aflatoxin B1 exposure caused a dose-related increase in embryonic mortality. After hatch, no differences were observed in body weight gain among treatment groups. The incidence of circulating thrombocytes was reduced in chicks exposed to the highest AFB1 dose with enhancement in monocyte and lymphocyte cell populations. Birds exposed to 1 microgram AFB1 recruited fewer macrophages in the peritoneal cavity after i.p. Sephadex elicitation along with reduced substrate adherence potential of peritoneal exudate cells. Similarly, macrophages from 0.5 and 1 micrograms AFB1-treated birds had depressed phagocytic potential. These results suggest that long-term immune depression of macrophage-mediated functions can occur following embryonic exposure to AFB1.
authors.affiliation: Department of Poultry Science, North Carolina State University, Raleigh 27695-7608.
authors.forename: D L
authors.initials: DL
authors.lastname: Neldon-Ortiz
authors.forename: M A
authors.initials: MA
authors.lastname: Qureshi
mesh_terms: D016604:Aflatoxin B1 / Q000494:pharmacology* / Q000633:toxicity; D000367:Age Factors; D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D007153:Immunologic Deficiency Syndromes / Q000139:chemically induced; D007958:Leukocyte Count / Q000187:drug effects; D008264:Macrophages / Q000187:drug effects*; D015651:Mycotoxicosis / Q000209:etiology / Q000276:immunology*; D010587:Phagocytosis / Q000187:drug effects*
chemical_list: D016604:Aflatoxin B1