Monoclonal antibody based capture ELISA/ELIFA for the detection of Chlamydia psittaci in veterinary clinical specimens.
A capture ELISA/ELIFA system based on monoclonal capture and biotinylated monoclonal detection antibody with specificity for an epitope on chlamydial lipopolysaccharide (LPS) is described. The assay is fast, specific and detects a minimum dose of approximately 2000 chlamydial particles (C. psittaci). Unlike other commercially available test kits, it comprises a proteinase K treatment to exclude non-specific binding of antibodies to Fc receptors. In contrast to the sophisticated and cumbersome isolation procedures, even non-specialized laboratories may use this assay system for the investigation of clinical samples of different origin for the presence of C. psittaci within a short period of time. Due to the genus specificity of the monoclonal antibody used C. trachomatis and C. pneumoniae can be detected as well.
Thiele
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D
Institut für Hygiene und Infektionskrankheiten der Tiere, Liebig-Universität Giessen, Germany.
Karo
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Krauss
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H
D000818:Animals; D000907:Antibodies, Bacterial / Q000737:chemistry*; D000911:Antibodies, Monoclonal / Q000737:chemistry*; D001666:Binding Sites, Antibody; D002415:Cats; D002417:Cattle; D002691:Chlamydophila psittaci / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006041:Goats; D006801:Humans; D010311:Parrots; D009956:Psittacosis / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary*; D011933:Reagent Kits, Diagnostic / Q000662:veterinary; D012756:Sheep; D013552:Swine
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D011933:Reagent Kits, Diagnostic