The primitive metazoan Hydra expresses antistasin, a serine protease inhibitor of vertebrate blood coagulation: cDNA cloning, cellular localisation and developmental regulation.
We have isolated and characterized cDNAs from Hydra which encode antistasin, a potent inhibitor of factor Xa in the vertebrate blood clotting cascade. Hydra antistasin is expressed in gland cells and represents a major class of transcripts from Hydra's head. Sequence analysis revealed that Hydra antistasin contains 6 internal repeats of a 25-26 amino acid sequence with a highly conserved pattern of 6 cysteine and 2 glycine residues identical to that in leech antistasin. Conservation of antistasin in a lower metazoan provides a potential link between the vertebrate and invertebrate coagulation systems.
Holstein
T W
TW
Zoologisches Institut, Universität München, Germany.
Mala
C
C
Kurz
E
E
Bauer
K
K
Greber
M
M
David
C N
CN
D000595:Amino Acid Sequence; D000818:Animals; D000925:Anticoagulants / Q000378:metabolism*; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D004247:DNA; D005786:Gene Expression Regulation; D006829:Hydra / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D007447:Invertebrate Hormones / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D012689:Sequence Homology, Nucleic Acid
D000925:Anticoagulants; D007447:Invertebrate Hormones; C053155:antistasin; D004247:DNA