The astrocyte--extracellular matrix complex in CNS myelinated tracts: a comparative study on the distribution of hyaluronate in rat, goldfish and lamprey.
The localization of hyaluronate was studied in the CNS of rat, goldfish and lamprey. Cryostat sections were incubated with glial hyaluronate-binding protein of human origin and stained by indirect immunofluorescence with glial hyaluronate binding protein antibodies not reaching with rat and fish. As previously reported for glial hyaluronate-binding protein and glial fibrillary acidic protein, hyaluronate and glial fibrillary acidic protein had a similar distribution in rat spinal cord and optic nerve, both substances forming ring-like structures around individual myelinated axons. A similar periaxonal distribution was observed in goldfish spinal cord and medulla, except that the rings were much wider, to accommodate the large goldfish axons. The glial fibrillary acidic protein-positive neuroglial tissue forming distinctive structures in goldfish vagal lobes also stained for hyaluronate. In both rat and goldfish spinal cord, motoneurons were surrounded by a hyaluronate coat. Goldfish optic nerve and lamprey spinal cord were hyaluronate-negative and, as previously reported, they stained for keratin but not for glial fibrillary acidic protein. The findings suggest that hyaluronate in CNS fibre tracts in a product of glial fibrillary acidic protein-positive neuroglia. They also suggest that the appearance of glial fibrillary acidic protein-positive neuroglia and the formation of a hyaluronate-bound extracellular matrix are related phenomena in phylogeny.
Bignami
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A
Department of Pathology, Harvard Medical School, Boston, MA.
Perides
G
G
Asher
R
R
Dahl
D
D
D000818:Animals; D001253:Astrocytes / Q000737:chemistry / Q000648:ultrastructure*; D001369:Axons / Q000648:ultrastructure*; D005109:Extracellular Matrix / Q000648:ultrastructure*; D005455:Fluorescent Antibody Technique; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D006054:Goldfish / Q000033:anatomy & histology*; D006820:Hyaluronic Acid / Q000032:analysis*; D007798:Lampreys / Q000033:anatomy & histology*; D009046:Motor Neurons / Q000737:chemistry / Q000648:ultrastructure; D009186:Myelin Sheath / Q000648:ultrastructure*; D009457:Neuroglia / Q000737:chemistry; D009900:Optic Nerve / Q000737:chemistry; D051381:Rats; D011919:Rats, Inbred Strains; D013045:Species Specificity; D013116:Spinal Cord / Q000737:chemistry
D005904:Glial Fibrillary Acidic Protein; D006820:Hyaluronic Acid