Regulation of heme pathway in regenerating mouse liver.
1. delta-Aminolevulinic acid synthetase (ALA-S), rhodanese and microsomal heme oxygenase (MHO), were quantitated in Cl4C induced regenerating mouse liver. 2. Maximal hepatomegalia was observed at 48 hr after i.p. injection of a single dose of the toxin. 3. ALA-S activity decreased on day 2, and then significantly increased (50%) between days 3 and 7, returning afterwards to control values. 4. Cytoplasmic rhodanese, as well as MHO activities, exhibited a clear correlation as compared with the ALA-S activity profile. 5. Porphyrin biosynthesis from precursor delta-aminolevulinic acid (ALA) was significantly increased even after 15 days of intoxication. 6. Present results would indicate that Cl4C is acting in a dual fashion.
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Centro de Investigaciones sobre Porfirinas y Porfirias (CIPYP), CONICET y FCEN, UBA, Ciudad Universitaria, Buenos Aires, Republica Argentina.
Vazquez
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D000624:5-Aminolevulinate Synthetase / Q000378:metabolism; D000622:Aminolevulinic Acid / Q000378:metabolism; D000818:Animals; D002251:Carbon Tetrachloride / Q000494:pharmacology; D003577:Cytochrome P-450 Enzyme System / Q000172:deficiency; D006418:Heme / Q000378:metabolism*; D006419:Heme Oxygenase (Decyclizing) / Q000378:metabolism; D007700:Kinetics; D008099:Liver / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D008115:Liver Regeneration / Q000187:drug effects / Q000502:physiology*; D051379:Mice; D008862:Microsomes, Liver / Q000187:drug effects / Q000201:enzymology; D011166:Porphyrins / Q000096:biosynthesis; D013884:Thiosulfate Sulfurtransferase / Q000378:metabolism
D011166:Porphyrins; D006418:Heme; D000622:Aminolevulinic Acid; D003577:Cytochrome P-450 Enzyme System; D002251:Carbon Tetrachloride; D006419:Heme Oxygenase (Decyclizing); D000624:5-Aminolevulinate Synthetase; D013884:Thiosulfate Sulfurtransferase