title: [An improved tetrazolium agar medium for testing sugar fermentation in lactobacilli].
abstract: An improved tetrazolium agar medium for testing sugar fermentation in lactobacilli is described. Basal medium 86 was essentially a modified MRS broth with the omission of glucose. The standard formula was 30 micrograms/ml of 2,3,5-triphenyltetrazolium chloride, 2% sugar to be tested, and 2% agar in medium 86. Plates were incubated anaerobically for 2 days at 37C or 5 days at 30C, depending on the strain. With three strains each of group II and III lactobacilli, colorless, fermentation-positive colonies were clearly differentiated from red, fermentation-negative colonies. For three strains of group I lactobacilli, this medium was not satisfactory because they grew poorly on it unless supplemented with a sugar.
authors.affiliation: Department of Microbiology, Kochi Medical School, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Nagasaki
authors.forename: K
authors.initials: K
authors.lastname: Ito
authors.forename: S
authors.initials: S
authors.lastname: Matsuzaki
authors.forename: S
authors.initials: S
authors.lastname: Tanaka
mesh_terms: D000362:Agar; D050260:Carbohydrate Metabolism; D003470:Culture Media; D005285:Fermentation; D007778:Lactobacillus / Q000254:growth & development / Q000378:metabolism*; D013778:Tetrazolium Salts
chemical_list: D003470:Culture Media; D013778:Tetrazolium Salts; C009591:triphenyltetrazolium; D000362:Agar