Effects of aluminum speciation on murine neuroblastoma cells.
Murine neuroblastoma cells behave differently in the presence of Al(acac)3 [acac = 2,4-pentanedionate; acetylacetonate] or Al(malt)3 [malt = 3-hydroxy, 2-methyl, 4-pyronate; maltolate] with respect to Al(lac)3 [lac = 2-hydroxypropionate; lactate]. Thus, a remarkable cytotoxic effect was observed in the first case; on the contrary, an evident cytostatic and neuritogenic effect was produced by aqueous Al(lac)3. The hydrolytically stable complexes Al(acac)3 and Al(malt)3 were both toxic in the concentration range of 0.10-0.30 and 0.10-0.50 mM, respectively, over 24 h. In contrast with this behavior Al(lac)3 displayed a potent cytostatic activity with induction of neurites at 0.2-10 mM. Al(OH)3 manifested biological effects comparable to those exhibited by Al(lac)3. AlPO4 was also cytostatic and led to a morphological differentiation of the neuroblastoma cells, qualitatively different from that elicited by Al(lac)3. The morphological effects induced by Al(lac)3, Al(OH)3, and AlPO4 were irreversible.
Zatta
P
P
CNR-Centro per lo Studio della Biochimica e della Fisiologia delle Emocianine ed Altre Metalloprotein, Universita' Padova, Italy.
Perazzolo
M
M
Facci
L
L
Skaper
S D
SD
Corain
B
B
Favarato
M
M
D000535:Aluminum / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D000544:Alzheimer Disease / Q000209:etiology; D000818:Animals; D016923:Cell Death / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D007773:Lactates / Q000494:pharmacology*; D019344:Lactic Acid; D051379:Mice; D009447:Neuroblastoma / Q000473:pathology*; D009942:Organometallic Compounds / Q000494:pharmacology*; D010422:Pentanones / Q000494:pharmacology*; D011753:Pyrones / Q000494:pharmacology*; D014407:Tumor Cells, Cultured / Q000187:drug effects
D007773:Lactates; D009942:Organometallic Compounds; D010422:Pentanones; D011753:Pyrones; C067527:aluminum maltolate; D019344:Lactic Acid; C058508:tris(acetylacetonate) aluminum(III); D000535:Aluminum