Preprothyrotropin-releasing hormone mRNA and TRH are present in the rat heart.
PreproTRH mRNA has been identified in rat cardiac tissues by Northern analyses and RNase protection assays with a specific rat 32P-TRH cRNA probe. Densitometric analyses revealed that atrial ppTRH mRNA concentrations were approximately five-fold greater than those of the ventricles. TRH concentrations (RIA), by contrast, were two-fold higher in ventricles. These data suggest that TRH and TRH mRNA are present in the rat heart, but their concentrations are dissociated, possibly because of differential post-transcriptional or post-translational processing. TRH is postulated to play an autocrine or paracrine role in cardiac physiology.
Carnell
N E
NE
Division of Endocrinology, University of Maryland School of Medicine, Baltimore 21201.
Feng
P
P
Kim
U J
UJ
Wilber
J F
JF
D000818:Animals; D015152:Blotting, Northern; D006325:Heart Atria / Q000737:chemistry; D006352:Heart Ventricles / Q000737:chemistry; D009206:Myocardium / Q000737:chemistry*; D011498:Protein Precursors / Q000235:genetics*; D015347:RNA Probes; D012333:RNA, Messenger / Q000032:analysis*; D051381:Rats; D012260:Ribonucleases; D013973:Thyrotropin-Releasing Hormone / Q000032:analysis* / Q000235:genetics*
D011498:Protein Precursors; D015347:RNA Probes; D012333:RNA, Messenger; C063596:prepro-thyrotropin-releasing hormone; D013973:Thyrotropin-Releasing Hormone; D012260:Ribonucleases