Molecular monitoring of the transcriptional activation of the yeast Saccharomyces kluyveri mating pheromone signal transduction by using FUS1-lacZ fusion gene.
To analyse the molecular mechanism by which a mating pheromone signal is transmitted in Saccharomyces kluyveri, the S. cerevisiae FUS1-lacZ fusion gene was introduced into S. kluyveri cells and its transcriptional activation was investigated. The expression of FUS1-lacZ fusion product was cell-type-specifically induced by pheromone treatment in S. kluyveri. The result suggests that S. kluyveri pheromone signal may be transduced by a mechanism similar to that in S. cerevisiae. This system may provide a model for analysing the molecular mechanism of the mating reaction in Saccharomyces kluyveri.
Fujimura
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Laboratory for Molecular Biology, Hoechst Japan Limited, Kawagoe, Japan.
D003227:Conjugation, Genetic; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D012440:Saccharomyces / Q000235:genetics*; D012724:Sex Attractants / Q000235:genetics*; D015398:Signal Transduction / Q000235:genetics*; D014158:Transcription, Genetic; D001616:beta-Galactosidase / Q000235:genetics*
D011993:Recombinant Fusion Proteins; D012724:Sex Attractants; D001616:beta-Galactosidase