In-vivo measurements of early and late effects in normal tissue after abdominal X-irradiation--development of a clinically orientated score for the determination of side-effects and the individual radiation response in a mouse model.
Heiligenberger mice received abdominal single X-ray doses of either 10 or 12 Gy. Early (up to one month) and late effects (two to eight months) were measured in vivo with ten parameters (nitrite, albumin, glucose, urobilinogen, bilirubin, haemoglobine, ketone, pH, weight development, general clinical conditions). These parameters permit the assessment of the condition of the essential organ systems. Thus, their use as screening parameters appears meaningful. By creating indices and integrating these in the animal index (range 1 to 3) it is possible to obtain an impression of the health condition of the individual animal. Early and late damage can be determined with certainty despite large individual variances. Radiation-induced chronic but not lethal changes can be measured. A large early damage is also predictive for a large late morbidity; not the individual parameters allow conclusions to be drawn but instead only the total number of the parameters used here are capable of this. The results have tended to show that the systematic listing of early responses also allows for very early indication of late morbidity or chronification events to be expected. The results have shown that the individual analysis of single parameters can give rise to important indications for a decisive diagnosis. This corresponds to the usual clinical procedure and can be applied to animal experiments without restriction.
Steinberg
F
F
Institute of Medical Radiation Biology, University Clinics, Essen.
Streffer
C
C
D000005:Abdomen / Q000528:radiation effects*; D000818:Animals; D004195:Disease Models, Animal; D004307:Dose-Response Relationship, Radiation; D006417:Hematuria / Q000145:classification / Q000652:urine; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009780:Occult Blood; D011379:Prognosis; D011833:Radiation Injuries, Experimental / Q000145:classification* / Q000652:urine; D013997:Time Factors