Expression of Bordetella pertussis filamentous hemagglutinin in Escherichia coli using a two cistron system.
Expression of Bordetella pertussis filamentous hemagglutinin (FHA) has been achieved in Escherichia coli K-12. This involved the construction of a two cistron system where the first cistron was provided by the NH2-terminus (first 98 amino acids) of MS2 polymerase. When the FHA gene sequences were fused to the first cistron, higher levels of expression were obtained and the fusion protein aggregated in inclusion bodies. FHA expressed by the two cistron system, however, appeared to be diffusely dispersed in the cytoplasm.
Guzmán
C A
CA
Department of Microbiology, GBF-National Research Centre for Biotechnology, Braunschweig, Germany.
Walker
M J
MJ
Rohde
M
M
Timmis
K N
KN
D018829:Adhesins, Bacterial; D001483:Base Sequence; D001886:Bordetella pertussis / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial / Q000235:genetics*; D006388:Hemagglutinins / Q000096:biosynthesis* / Q000235:genetics; D008969:Molecular Sequence Data; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics*; D010566:Virulence Factors, Bordetella
D018829:Adhesins, Bacterial; D006388:Hemagglutinins; D011993:Recombinant Fusion Proteins; D010566:Virulence Factors, Bordetella; C065273:filamentous hemagglutinin adhesin, Bordetella pertussis