title: Determination of nonderivatized para-hydroxylated metabolites of diazepam in biological fluids with a GC Megabore column system.
abstract: A method was developed using gas chromatography (GC) and a Megabore column system capable of simultaneous detection of diazepam, N-desmethyldiazepam, temazepam, oxazepam, and their para-hydroxylated metabolites. This method does not require derivatization of para-hydroxylated metabolites. Standard curves for pure reference compounds were linear, with the minimum detectable concentration of diazepam and its metabolites as low as 0.13 ng/injection.
authors.affiliation: Department of Pharmacology, Faculty of Medicine, University of Toronto, Canada.
authors.forename: T V
authors.initials: TV
authors.lastname: Beischlag
authors.forename: T
authors.initials: T
authors.lastname: Inaba
mesh_terms: D000818:Animals; D002849:Chromatography, Gas; D003975:Diazepam / Q000378:metabolism / Q000652:urine*; D006900:Hydroxylation; D008297:Male; D003708:Nordazepam / Q000652:urine*; D010076:Oxazepam / Q000652:urine*; D051381:Rats; D011919:Rats, Inbred Strains; D013693:Temazepam / Q000652:urine*
chemical_list: D003708:Nordazepam; D010076:Oxazepam; D013693:Temazepam; D003975:Diazepam