[Analysis of human growth hormone-binding protein in plasma by high pressure liquid chromatography].
A technique using high pressure liquid chromatography gel filtration was used to evaluate GH-binding proteins (GH-BPs) in human plasma; eluate was monitored for radioactivity in a gamma-detection system connected to a computer. Plasma (200 microliters) was incubated with 125I-human (h) GH (200,000 cpm) at 4 degrees C for 20 hours. Our GH binding assay offers important gains in terms of rapidity and resolution; it has permitted a clear separation and characterization of the two GH-binding components present in human plasma.
Tar
A
A
INSERM U.30, Hôpital des Enfants-Malades, Paris.
Hocquette
J F
JF
Souberbielle
J C
JC
Clot
J P
JP
Brauner
R
R
Postel-Vinay
M C
MC
D002352:Carrier Proteins / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D013006:Growth Hormone / Q000097:blood / Q000378:metabolism*; D006801:Humans; D011485:Protein Binding
D002352:Carrier Proteins; D013006:Growth Hormone; C051815:somatotropin-binding protein
A humán növekedési hormon kötöfehérjék vizsgálata plazmában magas nyomású folyadékkromatográfiával.